THBS1 cDNA ORF clone, Canis lupus familiaris(dog)

The following THBS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the THBS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe28631 XM_544610.6
Latest version!
Canis lupus familiaris thrombospondin 1 (THBS1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
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OCe28631 XM_544610.5 Canis lupus familiaris thrombospondin 1 (THBS1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe28631
    Clone ID Related Accession (Same CDS sequence) XM_544610.5 , XM_544610.6
    Accession Version XM_544610.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3513bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product thrombospondin-1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_544610.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGGGCTGG CCTGGGCACT CGGCGTCCTG TTCCTGTTGC GTGTGTGCGC CTCCAGCCGC 
    CTTCCAGAGT CTGGGGGAGA CAACAGCGTG TTCGACATCT TTGAACTCAC GGGGGCTGCC
    CGGAGGGGCT CTGGGCGCCG GCTGGTGAAG GGTCCTGACC CCTCCAGCCC AGCTTTCCGC
    ATCGAGGATG CCAACCTGAT CCCCCCCGTG CCTGATGACA AGTTCCAAGA CCTAGTGGAT
    GCTGTGCGCG CAGAGAAAGG CTTCCTTCTC CTGGCCTCCC TGAGGCAGAT GAAGAAGACC
    CGAGGCACGC TGCTGGCCAT AGAACGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGGTC
    TCCAACGGCA GAGCCGGCAC CCTGGACCTG AGCCTGACCG TGCAGGGCAT GCAACACGTG
    GTGTCGGTGG AAGAAGCGCT GCTGGCCACC GGCCAGTGGA AGAGCATCAC CCTGTTCGTG
    CAGGAGGACA GGGCCCAGCT GTACATTGAC TGTGAGAAGA TGGAGAACGC CGAGCTGGAT
    GTCCCCATCC AGAGCATCTT CACCAGGGAC CTGGCCAGCG TGGCCAGACT CCGCATCGCG
    AAAGGAGGGG TCAACGACAA CTTCCAGGGG GTGCTGCAGA ACGTGAGGTT TGTCTTTGGA
    ACCACACCAG AAGCCATTCT CAGAAACAAA GGCTGTTCCA GTTCGACCAA CGTCCTCCTC
    ACCCTCGATA ACAATGTGGT AAATGGCTCC AGCCCCGCCA TCCGCACTAA CTACATCGGC
    CACAAGACTA AGGATCTGCA AGCCATCTGC GGCATCTCTT GTGATGAGCT ATCCAGCATG
    GTTCTGGAGT TGAGGGGCCT GCGCACCATT GTGACCACAC TTCAGGACAG CATCCGCAAA
    GTGACTGAAG AGAACAAAGA ACTGGCCATT GAGCTGAGGC GGCCTCCCCT CTGCTACCAC
    AATGGAGTTC AGTACAGGAA CAATGAGGAG TGGACCGTCG ATAGTTGTAC CGAGTGCCGC
    TGTCAGAACT CCGTTACCAT CTGCAAGAAG GTGTCCTGCC CCATCATGCC CTGCTCCAAC
    GCCACAGTTC CTGACGGAGA ATGCTGCCCA CGGTGTTGGC CCAGCGACTC TGCGGATGAT
    GGCTGGTCCC CATGGTCCGA GTGGACCTCC TGCTCTGCGA CATGTGGCAA TGGAATCCAG
    CAGCGCGGGC GCTCCTGTGA TAGTCTCAAC AACCGATGCG AGGGCTCCTC CGTCCAGACG
    CGGACTTGCC ACATTCAGGA GTGCGACAAG AGATTTAAAC AGGATGGCGG CTGGAGCCAC
    TGGTCCCCGT GGTCGTCTTG TTCCGTGACG TGTGGTGATG GTGTGATCAC TAGAATTCGA
    CTCTGCAATT CTCCTAGCCC CCAGATGAAC GGGAAACCAT GTGAAGGCGA AGCCCGGGAG
    ACCAAGGCCT GCAAGAAAGA TGCCTGCCCC ATCAATGGAG GCTGGGGTCC CTGGTCACTG
    TGGGACATCT GTTCCGTTAC CTGTGGAGGA GGGGTGCAGA AACGTAGCCG GCTCTGCAAC
    AACCCCACAC CCCAGTTTGG AGGCAAGGAC TGTGTCGGTG ATGCGACCGA AAACCAGATT
    TGCAACAAGC AGGACTGTCC AATTGATGGA TGCCTGTCCA ATCCCTGCTT TGCTGGGGTC
    AAGTGTACTA GTTACCGCGA TGGCAGCTGG AAATGTGGAA CTTGCCCCCC GGGCTACAGC
    GGGAATGGCA TCCAGTGCAA AGACGTGGAT GAGTGCAAAG AAGTCCCTGA CGCCTGCTTC
    AACCACAACG GAGAGCACAG GTGCGAGAAC ACGGACCCCG GCTACAACTG CCTGCCCTGC
    CCACCACGCT TCACAGGCCC GCAGCCCTTC GGCCGCGGTG TAGAACATGC CACCGCCCAC
    AAACAGGTGT GTAAGCCCCG GAACCCCTGC ACAGATGGGA CACATGACTG CAACAAGAAT
    GCCAAGTGCA GCTACCTGGG CCACTACAGC GACCCTATGT ATCGCTGCGA GTGTAAGCCC
    GGCTATGCGG GCAACGGCAT CATCTGTGGG GAGGACACAG ACCTGGACGG CTGGCCCAAT
    GAGGACCTGG TGTGCGTGGC CAATGCCACC TACCACTGCA AAAAGGATAA CTGCCCCAAC
    CTTCCCAACT CTGGGCAGGA AGACTATGAC AAGGACGGGA TTGGCGACGC CTGTGATGAC
    GACGATGACA ATGATAAAAT CCCAGACGAC AGGGACAACT GTCCTTTCCA TTACAACCCA
    GCCCAGTATG ACTATGACAG AGATGATGTC GGAGACCGCT GTGACAACTG CCCCTACAAC
    CACAACCCAG ATCAGGCTGA CACGGACAAC AACGGGGAAG GAGATGCCTG CGCCGCAGAC
    ATCGATGGGG ACGGTATCCT CAATGAACGT GACAACTGTC AGTATGTCTA CAATGTGGAC
    CAGAGGGACA CTGATATGGA TGGGGTTGGA GATCAGTGTG ACAACTGCCC CCTGGAACAT
    AATCCAGATC AGCTTGACTC TGACTCAGAC CGCATTGGAG ATACCTGTGA CAACAATCAG
    GATATTGATG AAGACGGCCA CCAAAACAAC CTGGACAACT GTCCCTATGT GCCCAATGCC
    AACCAAGCTG ACCATGACAA AGATGGCAAG GGAGATGCCT GTGACCATGA TGATGACAAT
    GATGGCATTC CTGATGACAG GGACAACTGC AGACTTGTGC CCAATCCTGA CCAGAAGGAT
    TCCGATGGTG ATGGTCGAGG GGATGCTTGC AAAGACGATT TTGACCATGA CAATGTGCCA
    GACATTGATG ACATCTGTCC TGAAAACATT GATATCAGCG AGACTGATTT CCGCAGATTC
    CAGATGATTC CTCTAGATCC CAAAGGGACA TCCCAAAATG ACCCTAACTG GGTGGTACGT
    CATCAGGGTA AAGAACTCGT CCAGACCGTC AACTGTGATC CTGGACTTGC TGTAGGTTAT
    GATGAGTTCA ATGCTGTGGA CTTTAGTGGC ACCTTCTTCA TCAACACAGA GAGGGATGAT
    GACTATGCTG GATTTGTGTT TGGCTACCAG TCTAGCAGCC GCTTCTATGT GGTGATGTGG
    AAGCAAGTCA CACAGTCCTA CTGGGACACC AACCCCACTC GGGCTCAGGG ATACTCTGGC
    CTTTCCGTGA AAGTCGTGAA CTCTACCACC GGGCCAGGCG AGCACCTGCG GAATGCCCTG
    TGGCACACAG GAAACACCCC TGGCCAGGTG CGCACTCTGT GGCATGACCC TCGACACATA
    GGCTGGAAAG ATTTCACTGC CTACAGATGG CGTCTCAGCC ACCGGCCCAA GACGGGTTTC
    ATTCGCGTGG TGATGTATGA AGGGAAGAAA ATCATGGCTG ATTCAGGACC CATCTATGAC
    AAAACTTATG CTGGTGGTAG GCTAGGGTTG TTCGTCTTCT CTCAAGAAAT GGTGTTCTTC
    TCTGACCTGA AATACGAATG CAGAGATTCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_544610.3
    CDS121..3633
    Translation

    Target ORF information:

    RefSeq Version XM_544610.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris thrombospondin 1 (THBS1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_544610.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGGGCTGG CCTGGGCACT CGGCGTCCTG TTCCTGTTGC GTGTGTGCGC CTCCAGCCGC 
    CTTCCAGAGT CTGGGGGAGA CAACAGCGTG TTCGACATCT TTGAACTCAC GGGGGCTGCC
    CGGAGGGGCT CTGGGCGCCG GCTGGTGAAG GGTCCTGACC CCTCCAGCCC AGCTTTCCGC
    ATCGAGGATG CCAACCTGAT CCCCCCCGTG CCTGATGACA AGTTCCAAGA CCTAGTGGAT
    GCTGTGCGCG CAGAGAAAGG CTTCCTTCTC CTGGCCTCCC TGAGGCAGAT GAAGAAGACC
    CGAGGCACGC TGCTGGCCAT AGAACGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGGTC
    TCCAACGGCA GAGCCGGCAC CCTGGACCTG AGCCTGACCG TGCAGGGCAT GCAACACGTG
    GTGTCGGTGG AAGAAGCGCT GCTGGCCACC GGCCAGTGGA AGAGCATCAC CCTGTTCGTG
    CAGGAGGACA GGGCCCAGCT GTACATTGAC TGTGAGAAGA TGGAGAACGC CGAGCTGGAT
    GTCCCCATCC AGAGCATCTT CACCAGGGAC CTGGCCAGCG TGGCCAGACT CCGCATCGCG
    AAAGGAGGGG TCAACGACAA CTTCCAGGGG GTGCTGCAGA ACGTGAGGTT TGTCTTTGGA
    ACCACACCAG AAGCCATTCT CAGAAACAAA GGCTGTTCCA GTTCGACCAA CGTCCTCCTC
    ACCCTCGATA ACAATGTGGT AAATGGCTCC AGCCCCGCCA TCCGCACTAA CTACATCGGC
    CACAAGACTA AGGATCTGCA AGCCATCTGC GGCATCTCTT GTGATGAGCT ATCCAGCATG
    GTTCTGGAGT TGAGGGGCCT GCGCACCATT GTGACCACAC TTCAGGACAG CATCCGCAAA
    GTGACTGAAG AGAACAAAGA ACTGGCCATT GAGCTGAGGC GGCCTCCCCT CTGCTACCAC
    AATGGAGTTC AGTACAGGAA CAATGAGGAG TGGACCGTCG ATAGTTGTAC CGAGTGCCGC
    TGTCAGAACT CCGTTACCAT CTGCAAGAAG GTGTCCTGCC CCATCATGCC CTGCTCCAAC
    GCCACAGTTC CTGACGGAGA ATGCTGCCCA CGGTGTTGGC CCAGCGACTC TGCGGATGAT
    GGCTGGTCCC CATGGTCCGA GTGGACCTCC TGCTCTGCGA CATGTGGCAA TGGAATCCAG
    CAGCGCGGGC GCTCCTGTGA TAGTCTCAAC AACCGATGCG AGGGCTCCTC CGTCCAGACG
    CGGACTTGCC ACATTCAGGA GTGCGACAAG AGATTTAAAC AGGATGGCGG CTGGAGCCAC
    TGGTCCCCGT GGTCGTCTTG TTCCGTGACG TGTGGTGATG GTGTGATCAC TAGAATTCGA
    CTCTGCAATT CTCCTAGCCC CCAGATGAAC GGGAAACCAT GTGAAGGCGA AGCCCGGGAG
    ACCAAGGCCT GCAAGAAAGA TGCCTGCCCC ATCAATGGAG GCTGGGGTCC CTGGTCACTG
    TGGGACATCT GTTCCGTTAC CTGTGGAGGA GGGGTGCAGA AACGTAGCCG GCTCTGCAAC
    AACCCCACAC CCCAGTTTGG AGGCAAGGAC TGTGTCGGTG ATGCGACCGA AAACCAGATT
    TGCAACAAGC AGGACTGTCC AATTGATGGA TGCCTGTCCA ATCCCTGCTT TGCTGGGGTC
    AAGTGTACTA GTTACCGCGA TGGCAGCTGG AAATGTGGAA CTTGCCCCCC GGGCTACAGC
    GGGAATGGCA TCCAGTGCAA AGACGTGGAT GAGTGCAAAG AAGTCCCTGA CGCCTGCTTC
    AACCACAACG GAGAGCACAG GTGCGAGAAC ACGGACCCCG GCTACAACTG CCTGCCCTGC
    CCACCACGCT TCACAGGCCC GCAGCCCTTC GGCCGCGGTG TAGAACATGC CACCGCCCAC
    AAACAGGTGT GTAAGCCCCG GAACCCCTGC ACAGATGGGA CACATGACTG CAACAAGAAT
    GCCAAGTGCA GCTACCTGGG CCACTACAGC GACCCTATGT ATCGCTGCGA GTGTAAGCCC
    GGCTATGCGG GCAACGGCAT CATCTGTGGG GAGGACACAG ACCTGGACGG CTGGCCCAAT
    GAGGACCTGG TGTGCGTGGC CAATGCCACC TACCACTGCA AAAAGGATAA CTGCCCCAAC
    CTTCCCAACT CTGGGCAGGA AGACTATGAC AAGGACGGGA TTGGCGACGC CTGTGATGAC
    GACGATGACA ATGATAAAAT CCCAGACGAC AGGGACAACT GTCCTTTCCA TTACAACCCA
    GCCCAGTATG ACTATGACAG AGATGATGTC GGAGACCGCT GTGACAACTG CCCCTACAAC
    CACAACCCAG ATCAGGCTGA CACGGACAAC AACGGGGAAG GAGATGCCTG CGCCGCAGAC
    ATCGATGGGG ACGGTATCCT CAATGAACGT GACAACTGTC AGTATGTCTA CAATGTGGAC
    CAGAGGGACA CTGATATGGA TGGGGTTGGA GATCAGTGTG ACAACTGCCC CCTGGAACAT
    AATCCAGATC AGCTTGACTC TGACTCAGAC CGCATTGGAG ATACCTGTGA CAACAATCAG
    GATATTGATG AAGACGGCCA CCAAAACAAC CTGGACAACT GTCCCTATGT GCCCAATGCC
    AACCAAGCTG ACCATGACAA AGATGGCAAG GGAGATGCCT GTGACCATGA TGATGACAAT
    GATGGCATTC CTGATGACAG GGACAACTGC AGACTTGTGC CCAATCCTGA CCAGAAGGAT
    TCCGATGGTG ATGGTCGAGG GGATGCTTGC AAAGACGATT TTGACCATGA CAATGTGCCA
    GACATTGATG ACATCTGTCC TGAAAACATT GATATCAGCG AGACTGATTT CCGCAGATTC
    CAGATGATTC CTCTAGATCC CAAAGGGACA TCCCAAAATG ACCCTAACTG GGTGGTACGT
    CATCAGGGTA AAGAACTCGT CCAGACCGTC AACTGTGATC CTGGACTTGC TGTAGGTTAT
    GATGAGTTCA ATGCTGTGGA CTTTAGTGGC ACCTTCTTCA TCAACACAGA GAGGGATGAT
    GACTATGCTG GATTTGTGTT TGGCTACCAG TCTAGCAGCC GCTTCTATGT GGTGATGTGG
    AAGCAAGTCA CACAGTCCTA CTGGGACACC AACCCCACTC GGGCTCAGGG ATACTCTGGC
    CTTTCCGTGA AAGTCGTGAA CTCTACCACC GGGCCAGGCG AGCACCTGCG GAATGCCCTG
    TGGCACACAG GAAACACCCC TGGCCAGGTG CGCACTCTGT GGCATGACCC TCGACACATA
    GGCTGGAAAG ATTTCACTGC CTACAGATGG CGTCTCAGCC ACCGGCCCAA GACGGGTTTC
    ATTCGCGTGG TGATGTATGA AGGGAAGAAA ATCATGGCTG ATTCAGGACC CATCTATGAC
    AAAACTTATG CTGGTGGTAG GCTAGGGTTG TTCGTCTTCT CTCAAGAAAT GGTGTTCTTC
    TCTGACCTGA AATACGAATG CAGAGATTCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe28631
    Clone ID Related Accession (Same CDS sequence) XM_544610.5 , XM_544610.6
    Accession Version XM_544610.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3513bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product thrombospondin-1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_544610.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGGGCTGG CCTGGGCACT CGGCGTCCTG TTCCTGTTGC GTGTGTGCGC CTCCAGCCGC 
    CTTCCAGAGT CTGGGGGAGA CAACAGCGTG TTCGACATCT TTGAACTCAC GGGGGCTGCC
    CGGAGGGGCT CTGGGCGCCG GCTGGTGAAG GGTCCTGACC CCTCCAGCCC AGCTTTCCGC
    ATCGAGGATG CCAACCTGAT CCCCCCCGTG CCTGATGACA AGTTCCAAGA CCTAGTGGAT
    GCTGTGCGCG CAGAGAAAGG CTTCCTTCTC CTGGCCTCCC TGAGGCAGAT GAAGAAGACC
    CGAGGCACGC TGCTGGCCAT AGAACGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGGTC
    TCCAACGGCA GAGCCGGCAC CCTGGACCTG AGCCTGACCG TGCAGGGCAT GCAACACGTG
    GTGTCGGTGG AAGAAGCGCT GCTGGCCACC GGCCAGTGGA AGAGCATCAC CCTGTTCGTG
    CAGGAGGACA GGGCCCAGCT GTACATTGAC TGTGAGAAGA TGGAGAACGC CGAGCTGGAT
    GTCCCCATCC AGAGCATCTT CACCAGGGAC CTGGCCAGCG TGGCCAGACT CCGCATCGCG
    AAAGGAGGGG TCAACGACAA CTTCCAGGGG GTGCTGCAGA ACGTGAGGTT TGTCTTTGGA
    ACCACACCAG AAGCCATTCT CAGAAACAAA GGCTGTTCCA GTTCGACCAA CGTCCTCCTC
    ACCCTCGATA ACAATGTGGT AAATGGCTCC AGCCCCGCCA TCCGCACTAA CTACATCGGC
    CACAAGACTA AGGATCTGCA AGCCATCTGC GGCATCTCTT GTGATGAGCT ATCCAGCATG
    GTTCTGGAGT TGAGGGGCCT GCGCACCATT GTGACCACAC TTCAGGACAG CATCCGCAAA
    GTGACTGAAG AGAACAAAGA ACTGGCCATT GAGCTGAGGC GGCCTCCCCT CTGCTACCAC
    AATGGAGTTC AGTACAGGAA CAATGAGGAG TGGACCGTCG ATAGTTGTAC CGAGTGCCGC
    TGTCAGAACT CCGTTACCAT CTGCAAGAAG GTGTCCTGCC CCATCATGCC CTGCTCCAAC
    GCCACAGTTC CTGACGGAGA ATGCTGCCCA CGGTGTTGGC CCAGCGACTC TGCGGATGAT
    GGCTGGTCCC CATGGTCCGA GTGGACCTCC TGCTCTGCGA CATGTGGCAA TGGAATCCAG
    CAGCGCGGGC GCTCCTGTGA TAGTCTCAAC AACCGATGCG AGGGCTCCTC CGTCCAGACG
    CGGACTTGCC ACATTCAGGA GTGCGACAAG AGATTTAAAC AGGATGGCGG CTGGAGCCAC
    TGGTCCCCGT GGTCGTCTTG TTCCGTGACG TGTGGTGATG GTGTGATCAC TAGAATTCGA
    CTCTGCAATT CTCCTAGCCC CCAGATGAAC GGGAAACCAT GTGAAGGCGA AGCCCGGGAG
    ACCAAGGCCT GCAAGAAAGA TGCCTGCCCC ATCAATGGAG GCTGGGGTCC CTGGTCACTG
    TGGGACATCT GTTCCGTTAC CTGTGGAGGA GGGGTGCAGA AACGTAGCCG GCTCTGCAAC
    AACCCCACAC CCCAGTTTGG AGGCAAGGAC TGTGTCGGTG ATGCGACCGA AAACCAGATT
    TGCAACAAGC AGGACTGTCC AATTGATGGA TGCCTGTCCA ATCCCTGCTT TGCTGGGGTC
    AAGTGTACTA GTTACCGCGA TGGCAGCTGG AAATGTGGAA CTTGCCCCCC GGGCTACAGC
    GGGAATGGCA TCCAGTGCAA AGACGTGGAT GAGTGCAAAG AAGTCCCTGA CGCCTGCTTC
    AACCACAACG GAGAGCACAG GTGCGAGAAC ACGGACCCCG GCTACAACTG CCTGCCCTGC
    CCACCACGCT TCACAGGCCC GCAGCCCTTC GGCCGCGGTG TAGAACATGC CACCGCCCAC
    AAACAGGTGT GTAAGCCCCG GAACCCCTGC ACAGATGGGA CACATGACTG CAACAAGAAT
    GCCAAGTGCA GCTACCTGGG CCACTACAGC GACCCTATGT ATCGCTGCGA GTGTAAGCCC
    GGCTATGCGG GCAACGGCAT CATCTGTGGG GAGGACACAG ACCTGGACGG CTGGCCCAAT
    GAGGACCTGG TGTGCGTGGC CAATGCCACC TACCACTGCA AAAAGGATAA CTGCCCCAAC
    CTTCCCAACT CTGGGCAGGA AGACTATGAC AAGGACGGGA TTGGCGACGC CTGTGATGAC
    GACGATGACA ATGATAAAAT CCCAGACGAC AGGGACAACT GTCCTTTCCA TTACAACCCA
    GCCCAGTATG ACTATGACAG AGATGATGTC GGAGACCGCT GTGACAACTG CCCCTACAAC
    CACAACCCAG ATCAGGCTGA CACGGACAAC AACGGGGAAG GAGATGCCTG CGCCGCAGAC
    ATCGATGGGG ACGGTATCCT CAATGAACGT GACAACTGTC AGTATGTCTA CAATGTGGAC
    CAGAGGGACA CTGATATGGA TGGGGTTGGA GATCAGTGTG ACAACTGCCC CCTGGAACAT
    AATCCAGATC AGCTTGACTC TGACTCAGAC CGCATTGGAG ATACCTGTGA CAACAATCAG
    GATATTGATG AAGACGGCCA CCAAAACAAC CTGGACAACT GTCCCTATGT GCCCAATGCC
    AACCAAGCTG ACCATGACAA AGATGGCAAG GGAGATGCCT GTGACCATGA TGATGACAAT
    GATGGCATTC CTGATGACAG GGACAACTGC AGACTTGTGC CCAATCCTGA CCAGAAGGAT
    TCCGATGGTG ATGGTCGAGG GGATGCTTGC AAAGACGATT TTGACCATGA CAATGTGCCA
    GACATTGATG ACATCTGTCC TGAAAACATT GATATCAGCG AGACTGATTT CCGCAGATTC
    CAGATGATTC CTCTAGATCC CAAAGGGACA TCCCAAAATG ACCCTAACTG GGTGGTACGT
    CATCAGGGTA AAGAACTCGT CCAGACCGTC AACTGTGATC CTGGACTTGC TGTAGGTTAT
    GATGAGTTCA ATGCTGTGGA CTTTAGTGGC ACCTTCTTCA TCAACACAGA GAGGGATGAT
    GACTATGCTG GATTTGTGTT TGGCTACCAG TCTAGCAGCC GCTTCTATGT GGTGATGTGG
    AAGCAAGTCA CACAGTCCTA CTGGGACACC AACCCCACTC GGGCTCAGGG ATACTCTGGC
    CTTTCCGTGA AAGTCGTGAA CTCTACCACC GGGCCAGGCG AGCACCTGCG GAATGCCCTG
    TGGCACACAG GAAACACCCC TGGCCAGGTG CGCACTCTGT GGCATGACCC TCGACACATA
    GGCTGGAAAG ATTTCACTGC CTACAGATGG CGTCTCAGCC ACCGGCCCAA GACGGGTTTC
    ATTCGCGTGG TGATGTATGA AGGGAAGAAA ATCATGGCTG ATTCAGGACC CATCTATGAC
    AAAACTTATG CTGGTGGTAG GCTAGGGTTG TTCGTCTTCT CTCAAGAAAT GGTGTTCTTC
    TCTGACCTGA AATACGAATG CAGAGATTCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_544610.3
    CDS117..3629
    Translation

    Target ORF information:

    RefSeq Version XM_544610.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris thrombospondin 1 (THBS1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_544610.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGGGCTGG CCTGGGCACT CGGCGTCCTG TTCCTGTTGC GTGTGTGCGC CTCCAGCCGC 
    CTTCCAGAGT CTGGGGGAGA CAACAGCGTG TTCGACATCT TTGAACTCAC GGGGGCTGCC
    CGGAGGGGCT CTGGGCGCCG GCTGGTGAAG GGTCCTGACC CCTCCAGCCC AGCTTTCCGC
    ATCGAGGATG CCAACCTGAT CCCCCCCGTG CCTGATGACA AGTTCCAAGA CCTAGTGGAT
    GCTGTGCGCG CAGAGAAAGG CTTCCTTCTC CTGGCCTCCC TGAGGCAGAT GAAGAAGACC
    CGAGGCACGC TGCTGGCCAT AGAACGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGGTC
    TCCAACGGCA GAGCCGGCAC CCTGGACCTG AGCCTGACCG TGCAGGGCAT GCAACACGTG
    GTGTCGGTGG AAGAAGCGCT GCTGGCCACC GGCCAGTGGA AGAGCATCAC CCTGTTCGTG
    CAGGAGGACA GGGCCCAGCT GTACATTGAC TGTGAGAAGA TGGAGAACGC CGAGCTGGAT
    GTCCCCATCC AGAGCATCTT CACCAGGGAC CTGGCCAGCG TGGCCAGACT CCGCATCGCG
    AAAGGAGGGG TCAACGACAA CTTCCAGGGG GTGCTGCAGA ACGTGAGGTT TGTCTTTGGA
    ACCACACCAG AAGCCATTCT CAGAAACAAA GGCTGTTCCA GTTCGACCAA CGTCCTCCTC
    ACCCTCGATA ACAATGTGGT AAATGGCTCC AGCCCCGCCA TCCGCACTAA CTACATCGGC
    CACAAGACTA AGGATCTGCA AGCCATCTGC GGCATCTCTT GTGATGAGCT ATCCAGCATG
    GTTCTGGAGT TGAGGGGCCT GCGCACCATT GTGACCACAC TTCAGGACAG CATCCGCAAA
    GTGACTGAAG AGAACAAAGA ACTGGCCATT GAGCTGAGGC GGCCTCCCCT CTGCTACCAC
    AATGGAGTTC AGTACAGGAA CAATGAGGAG TGGACCGTCG ATAGTTGTAC CGAGTGCCGC
    TGTCAGAACT CCGTTACCAT CTGCAAGAAG GTGTCCTGCC CCATCATGCC CTGCTCCAAC
    GCCACAGTTC CTGACGGAGA ATGCTGCCCA CGGTGTTGGC CCAGCGACTC TGCGGATGAT
    GGCTGGTCCC CATGGTCCGA GTGGACCTCC TGCTCTGCGA CATGTGGCAA TGGAATCCAG
    CAGCGCGGGC GCTCCTGTGA TAGTCTCAAC AACCGATGCG AGGGCTCCTC CGTCCAGACG
    CGGACTTGCC ACATTCAGGA GTGCGACAAG AGATTTAAAC AGGATGGCGG CTGGAGCCAC
    TGGTCCCCGT GGTCGTCTTG TTCCGTGACG TGTGGTGATG GTGTGATCAC TAGAATTCGA
    CTCTGCAATT CTCCTAGCCC CCAGATGAAC GGGAAACCAT GTGAAGGCGA AGCCCGGGAG
    ACCAAGGCCT GCAAGAAAGA TGCCTGCCCC ATCAATGGAG GCTGGGGTCC CTGGTCACTG
    TGGGACATCT GTTCCGTTAC CTGTGGAGGA GGGGTGCAGA AACGTAGCCG GCTCTGCAAC
    AACCCCACAC CCCAGTTTGG AGGCAAGGAC TGTGTCGGTG ATGCGACCGA AAACCAGATT
    TGCAACAAGC AGGACTGTCC AATTGATGGA TGCCTGTCCA ATCCCTGCTT TGCTGGGGTC
    AAGTGTACTA GTTACCGCGA TGGCAGCTGG AAATGTGGAA CTTGCCCCCC GGGCTACAGC
    GGGAATGGCA TCCAGTGCAA AGACGTGGAT GAGTGCAAAG AAGTCCCTGA CGCCTGCTTC
    AACCACAACG GAGAGCACAG GTGCGAGAAC ACGGACCCCG GCTACAACTG CCTGCCCTGC
    CCACCACGCT TCACAGGCCC GCAGCCCTTC GGCCGCGGTG TAGAACATGC CACCGCCCAC
    AAACAGGTGT GTAAGCCCCG GAACCCCTGC ACAGATGGGA CACATGACTG CAACAAGAAT
    GCCAAGTGCA GCTACCTGGG CCACTACAGC GACCCTATGT ATCGCTGCGA GTGTAAGCCC
    GGCTATGCGG GCAACGGCAT CATCTGTGGG GAGGACACAG ACCTGGACGG CTGGCCCAAT
    GAGGACCTGG TGTGCGTGGC CAATGCCACC TACCACTGCA AAAAGGATAA CTGCCCCAAC
    CTTCCCAACT CTGGGCAGGA AGACTATGAC AAGGACGGGA TTGGCGACGC CTGTGATGAC
    GACGATGACA ATGATAAAAT CCCAGACGAC AGGGACAACT GTCCTTTCCA TTACAACCCA
    GCCCAGTATG ACTATGACAG AGATGATGTC GGAGACCGCT GTGACAACTG CCCCTACAAC
    CACAACCCAG ATCAGGCTGA CACGGACAAC AACGGGGAAG GAGATGCCTG CGCCGCAGAC
    ATCGATGGGG ACGGTATCCT CAATGAACGT GACAACTGTC AGTATGTCTA CAATGTGGAC
    CAGAGGGACA CTGATATGGA TGGGGTTGGA GATCAGTGTG ACAACTGCCC CCTGGAACAT
    AATCCAGATC AGCTTGACTC TGACTCAGAC CGCATTGGAG ATACCTGTGA CAACAATCAG
    GATATTGATG AAGACGGCCA CCAAAACAAC CTGGACAACT GTCCCTATGT GCCCAATGCC
    AACCAAGCTG ACCATGACAA AGATGGCAAG GGAGATGCCT GTGACCATGA TGATGACAAT
    GATGGCATTC CTGATGACAG GGACAACTGC AGACTTGTGC CCAATCCTGA CCAGAAGGAT
    TCCGATGGTG ATGGTCGAGG GGATGCTTGC AAAGACGATT TTGACCATGA CAATGTGCCA
    GACATTGATG ACATCTGTCC TGAAAACATT GATATCAGCG AGACTGATTT CCGCAGATTC
    CAGATGATTC CTCTAGATCC CAAAGGGACA TCCCAAAATG ACCCTAACTG GGTGGTACGT
    CATCAGGGTA AAGAACTCGT CCAGACCGTC AACTGTGATC CTGGACTTGC TGTAGGTTAT
    GATGAGTTCA ATGCTGTGGA CTTTAGTGGC ACCTTCTTCA TCAACACAGA GAGGGATGAT
    GACTATGCTG GATTTGTGTT TGGCTACCAG TCTAGCAGCC GCTTCTATGT GGTGATGTGG
    AAGCAAGTCA CACAGTCCTA CTGGGACACC AACCCCACTC GGGCTCAGGG ATACTCTGGC
    CTTTCCGTGA AAGTCGTGAA CTCTACCACC GGGCCAGGCG AGCACCTGCG GAATGCCCTG
    TGGCACACAG GAAACACCCC TGGCCAGGTG CGCACTCTGT GGCATGACCC TCGACACATA
    GGCTGGAAAG ATTTCACTGC CTACAGATGG CGTCTCAGCC ACCGGCCCAA GACGGGTTTC
    ATTCGCGTGG TGATGTATGA AGGGAAGAAA ATCATGGCTG ATTCAGGACC CATCTATGAC
    AAAACTTATG CTGGTGGTAG GCTAGGGTTG TTCGTCTTCT CTCAAGAAAT GGTGTTCTTC
    TCTGACCTGA AATACGAATG CAGAGATTCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.