ARHGEF28 cDNA ORF clone, Canis lupus familiaris(dog)

The following ARHGEF28 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARHGEF28 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe03223 XM_022410512.1
Latest version!
Canis lupus familiaris Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe35385 XM_014109078.2
Latest version!
Canis lupus familiaris Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe03220 XM_005617471.3
Latest version!
Canis lupus familiaris Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe03225 XM_005617476.3
Latest version!
Canis lupus familiaris Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCe286782 XM_022410517.1
Latest version!
Canis lupus familiaris Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCe35379 XM_014109055.2
Latest version!
Canis lupus familiaris Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe35380 XM_014109057.2
Latest version!
Canis lupus familiaris Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe35381 XM_014109060.2
Latest version!
Canis lupus familiaris Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe35382 XM_014109061.2
Latest version!
Canis lupus familiaris Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe35383 XM_014109065.2
Latest version!
Canis lupus familiaris Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe35384 XM_014109073.2
Latest version!
Canis lupus familiaris Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe35386 XM_014109079.1
Latest version!
Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 28 (ARHGEF28), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCe35387 XM_022410519.1
Latest version!
Canis lupus familiaris Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCe35387 XM_014109081.2
Latest version!
Canis lupus familiaris Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCe35388 XM_014109082.2
Latest version!
Canis lupus familiaris Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
View Old Accession Versions >>
OCe35380 XM_014109057.1 Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 28 (ARHGEF28), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe03225 XM_005617476.2 Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 28 (ARHGEF28), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCe35379 XM_014109055.1 Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 28 (ARHGEF28), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe35381 XM_014109060.1 Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 28 (ARHGEF28), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe35382 XM_014109061.1 Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 28 (ARHGEF28), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe35384 XM_014109073.1 Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 28 (ARHGEF28), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe03220 XM_005617471.2 Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 28 (ARHGEF28), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe35388 XM_014109082.1 Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 28 (ARHGEF28), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCe35387 XM_014109081.1 Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 28 (ARHGEF28), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCe35385 XM_014109078.1 Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 28 (ARHGEF28), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe35383 XM_014109065.1 Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 28 (ARHGEF28), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe03223
    Clone ID Related Accession (Same CDS sequence) XM_022410512.1
    Accession Version XM_022410512.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4734bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rho guanine nucleotide exchange factor 28 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGTTGA GCTGCAGCGA AGTACCTCTT TATGGGCAGA TGACCGTCTA TGCAAAGTTT 
    GGGAAAAATG TTTATCTTCC TAAAGATGCC GAGTTTTACT TTATTTATAA TGGATCTCAT
    CAGAGACACG TCGTGATTGC AAAACGCACT GAGGATAATG TTCTCCAGTC CAGCATTCCA
    GGGCATGGGC TTCAGGAGAC GGTGACGGTG TCTGTATGCC TCTGCTCAGA AGGTTACTCC
    CCAGTGACCA TGGGCTCCGG CTCAGTGACC TACGTGGACA ACATGGCTTG CAGGCTGGCT
    CGTTTGCTGG TCACCCAGGC TGATCGCCTC ACAGCCAGTA GTCATCAGAC CTTTCTGACT
    CCATTTGCCC TGACGGTGGG AGCATTGCCT GCTTTGGACG AGGAGCTGGT GCTGGCTCTG
    ACCCATCTGG AGTTGCCTCT TGAGTGGAGT GTCTTGGGAA ATTCTTCACT TGAAGTTTCT
    TTATGCAGAG AGTCTCTTCT ACACCTGGCT ATGAGATGGG GCCTGCCTAA GCTCTCTCAT
    TTCCTCTTGT GCCTCCCTGG GGGAGTTCAG GCTTTGTCTT TACCCAATGA AGAAGGTGCC
    ACACCATTAG ACTTGGCTTT ACGTGCCGGA CACTCCAAGC TGGTTGAAGA CATCACAAAT
    TTCCAGGGCA GACGTTCCCC CGACCTCTCC CGAGTGCACC TCGGCGGACG TGCCTTCCTG
    CAGTATGTTC ACCCGTCGGG GACACTGACG CTGACGCTGA ACCACACAGC TGAGCATTTG
    TTGGAGGCAG ATATTAAACT CTTCAGGAAA TACTTTTTGG ACAGAGCCTT TCTCCTCAAG
    GCTCTTGACG ACCGAGAAGC TGGGCCAGAG GAGAGCCGGG GCATGCCCTC CCGGGCTGCC
    GCCACTGAGG AGGGTACGCG AGCTGCCTCC CCCGCGCCCA GGAACCCAGA AGAGATTAGG
    AATTTGGTGA CCGGCAGGCC ATCACCTGAA AAGGAGGATG TAAAGCGTGT GGACAGCCCG
    GACGTTGACC CCCATGAACA CGACGGCCGG GACAGTCTTG ATTTGGATCA CTCTTTCGAT
    GTCTTCAAAA AACCTAAGCA TCCCCCAGCG TTCTTTGCTG CAGGCGGGCT CTCTGATGTG
    CTGAATGGCG GCGATGAAGT TTACGCCAAC TGTATGGTGA TTGACCAGGT TGGTGATTTG
    GATATTAACT ACATCAATTT AGAAGGAATC TCTACAGACG CCTGTGCTGA ATCCATGGGT
    TCTACTCTGG GGCCTCAGAA CTGCGAGCAT ATCCTTGCTG CTGAAACCAG CCATGGGAAA
    TACCCACTAA GTGAGAACAA GGAAGTGGTC TCAGATACCG AAGCTCAGCA GTCCTTCCCA
    TCACCGTCTA GTTCATATGC TTCCAATTTT GATCTTTCCT TTGGTCTTCA TGGATTTGAG
    AAGGAACAAA GTCTCCTGAA GAAAAGAAGC TCCAGCCTGG ATGCCTTGGA TGCCGACAGC
    GAAGGGGAAG GGCATTCTGA GCGGTCACAC ATCTGCTACA CGCCAGCATC TCAGAGTTCC
    TCGAGGACTG GGATTCCCAG TGGGGATGAG CTGGACTCCT TCGAGACCGC CACCAAACCT
    GATTTTAGTA TCTCCAGGAC AGAGTCGCTT TCTTTATCAA GTAGCCTGCA GTTGAAGGAA
    TCATTGCTTC CTGGAGTCCG CTCACGTTCT TATTCCTGCT CATCACCCAA AATTTCTTTA
    GGAAAAACTC GATTGGTGCG TGATTTCACA GTGTGCAATT CTGGTGAAGA GCAAAGAGCC
    TTCGGCTTCC CGGAGCCCGC CAGGGAGAGG AGGATTCAAG AGGAAGAATG GGATAAATAC
    ATCATACCTG CCAAGTCAGA GTCTGAGAAA TACAAAGTGA GCCGGACCTT CAGCTTCCTC
    ATGAACAGGA TGACAAGCCC TCGGAACAAA TCTAAGACGA AAAGCAAGGA TGCCAAAGAC
    AAAGAGAAAC CGAGCCGACA TCAGTTTGTC CCCGGAACCT TCTCTGGGGT TCTCCAGTGT
    TCCGTTTGTG ATAAAACGCT CCTGGGGAAG GAGTCATCCC AGTGTTCCAA CTGTAATGCA
    AACGTCCACA AGGGTTGTAA GGACGCTGCA CCTCCGTGTA CCAAGAAATT CCAGGAGAAA
    TATAACAAGA ACAAACCGCA GACCATCCTT GGAAACTCTT CATTTCGAGA CATCCCGCAA
    CCCAGTCTCT CCTTGCACCC TTCTTCCTCC ATGCCTATCG GATTGCCCAC TGGGAGAAAA
    GAAGCTTCTG CCCAGGCCCA TCCCTTGTCC AGAAGTGTTC CAGGCACTGC CTTGGAAAGC
    TTCAGAAGAT CGGGGCTATC CCTGGAGTCT GAGAGTGACG GGAACAGCTG GAGAAGCAGG
    TCTCATTCCG ATGAGCTGTT ACAGTCCATG GGCTCTTCTC CCTCCACGGA ATCCTTCTTA
    ATGGAAGATG TCGTGGACTC TTCTCTATGG AGTGACCTCA GCAGTGACGC CCAGGAGTTG
    GAAGCAGAAT CCTGGAGTCT GGTGGTGGAC CCCTCCTTTT GCAATAAGCA GGAGAAAGAT
    GTCATCAAAA GACAGGATGT CATTTTCGAG CTTATGCAAA CAGAGATGCA TCATATCCAG
    ACCCTGCTCA TCATGTCTGA GATCTTCAGG AAAGGAATGA AGGAGGAGCT GCAGCTTGAT
    CACAGCACCG TGGATAAAAT CTTTCCCTGC TTAGATGAGT TACTTGAAAT CCACAGGCAT
    TTCTTTTATA GTATGAAGGA GCGAAGGCAG GAGTCCTGTG CTGGTGGTGA CAGGAATTTT
    GTCATCAGCC GAATTGGAGA TATTCTAGTA CAGCAGTTTT CAGAAGAAAA TGCAAATCAA
    ATGAAGAAAA TATATGGAGA ATTCTGTAGC CATCACAAAG AAGCTGTTAG CCTCTATAAA
    GAACTCCAAC AGAATAAGAA GTTTCAGAAT TTTATTAAGC TTCGAAATAG TAATCTTTTG
    GCTCGACGCC GAGGAATCCC AGAATGCATT CTGCTAGTCA CTCAGCGCAT CACAAAATAC
    CCTGTCCTGG TGGAACGGAT TTTGCAGTAC ACAAAGGAAA GAAGTGAAGA ACATCAAGAC
    TTGTGCAAAG CTCTGTGCTT AATTAAAGAC ATGATTGCAG CAGTGGATTT AAAAGTCAGC
    GAGTATGAGA AAAACCAAAA ATGGCTTGAG ATCCTAAATA AGATCGAAAA CAAAACCTAC
    ACAAAGCTCA AAAATGGCCA CGTGTTTAGG AAGCAAGCAC TGATAAGTAA AGAAAGGACT
    CTCTTATATG ATGGCCTTGT TTATTGGAAA ACTGCTACGG GTCGTTTCAA AGATATCCTG
    GCTCTACTTC TAACTGATGT GCTGCTCTTT TTACAAGAAA AAGACCAGAA ATACATCTTT
    GCAGCTGTTG ATCAGAAGCC ATCGGTTATT TCCCTTCAGA AGCTCATTGC TAGAGAAGTC
    GCCAACGAGG AAAGAGGAAT GTTCCTGATC AGTGCATCCT CTGCTGGTCC TGAGATGTAC
    GAAATTCACA CCAACTCCAA GGAGGAACGA AATAACTGGA TGAGACGAAT CCAACAGGCC
    GTGGAAAGTT GCCCAGAAGA AGAAGGGGGA AGGACAAGTG AATCTGATGA AGAGAGGCGG
    AAAGCTGAAG CAAGAGTGGC CAAAATCCAG CAATGTCAAG AAATACTCAG TAACCAAGAC
    CAACAGATTT GCGCATATTT GGAGGAGAAG CTGCATATCT ACGCTGAACT TGGAGAACTG
    AGTGGATTCA AGGATGTCCA TCTAGAGCCC CACCTCCTCA TCAAACCTGA CCCAGGGGAG
    CCTCCCCAGG CAGCCTCATT ACTAGCAGCA GCCCTGAGAG AAGCGGAGAG CCTGCAGGTT
    GCCGTGAAGG CCTCACAAGC GGGTGATGCT CGCCCATCAC CTGAGGAGGG TTCTGCAGAA
    CCTGCGGTTG CAGATGCACA GGGTCCTCCC GAGGAGCCAG GATCATCTCC AGCCTCACTA
    GTCACAGAAG GGACAGGAGG AAGAGGCTGT TGGGATGTGG ATCCTGGCCC CCAGGGTGTG
    GGAACCGACT TGGCGGTCTC TGATGCAGGG GAGAAGGTGG AATATAGAAG TTTCACGGTT
    TCTTCACAGT CAGAGATTAT ACAAGCGATC CAGAATTTAA CCCGACTCCT CTATAGCCTT
    CAGGTGATGG AACTTAATCG ATCCGAGAGC TTGTGTCATG AAAATTCATT CTTCATTAAT
    GAAGCTTTAG TACAAATGTC ACTTAACACT TACAACAAAT CAACTCCATC AGGTGTCCAC
    CAGGAAGCCG CTTATCCCCA GAATATAGCT AATTCGGACT TGGTAAGGAC TAGTGAAAGT
    CCCGTAGACC TCCAGTTGGA CATTTCTCAG CCCTTGGATG TCAACCACGA ACTGTGGACA
    GCCGCTGGGT CCTGTCATCA GATACCCCCT CTCCACGAAA GCAGCAAGGA TTCTTGTAAA
    AATGGAAGAA TTTACATCAG TCAGCTATGT TTCCAGAGCC ATTCGTCAGA AATGTTTTGG
    CGACCAGACT TGACCATCTC AGCTTGGGAT AGAGAGCTTC CGAATCTGCA GAACTTCCAA
    CCGCTGTTGG TTGCATTATG TAGATGGTTG CGTTACGTCC GTACATCCCC GGGAGATCTT
    GCCGTCATCC TCGAGTCCCC TGAGCAGGTT TTTCAGCTAG AAAGAAAATG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022266220.1
    CDS85..4818
    Translation

    Target ORF information:

    RefSeq Version XM_022410512.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022410512.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGTTGA GCTGCAGCGA AGTACCTCTT TATGGGCAGA TGACCGTCTA TGCAAAGTTT 
    GGGAAAAATG TTTATCTTCC TAAAGATGCC GAGTTTTACT TTATTTATAA TGGATCTCAT
    CAGAGACACG TCGTGATTGC AAAACGCACT GAGGATAATG TTCTCCAGTC CAGCATTCCA
    GGGCATGGGC TTCAGGAGAC GGTGACGGTG TCTGTATGCC TCTGCTCAGA AGGTTACTCC
    CCAGTGACCA TGGGCTCCGG CTCAGTGACC TACGTGGACA ACATGGCTTG CAGGCTGGCT
    CGTTTGCTGG TCACCCAGGC TGATCGCCTC ACAGCCAGTA GTCATCAGAC CTTTCTGACT
    CCATTTGCCC TGACGGTGGG AGCATTGCCT GCTTTGGACG AGGAGCTGGT GCTGGCTCTG
    ACCCATCTGG AGTTGCCTCT TGAGTGGAGT GTCTTGGGAA ATTCTTCACT TGAAGTTTCT
    TTATGCAGAG AGTCTCTTCT ACACCTGGCT ATGAGATGGG GCCTGCCTAA GCTCTCTCAT
    TTCCTCTTGT GCCTCCCTGG GGGAGTTCAG GCTTTGTCTT TACCCAATGA AGAAGGTGCC
    ACACCATTAG ACTTGGCTTT ACGTGCCGGA CACTCCAAGC TGGTTGAAGA CATCACAAAT
    TTCCAGGGCA GACGTTCCCC CGACCTCTCC CGAGTGCACC TCGGCGGACG TGCCTTCCTG
    CAGTATGTTC ACCCGTCGGG GACACTGACG CTGACGCTGA ACCACACAGC TGAGCATTTG
    TTGGAGGCAG ATATTAAACT CTTCAGGAAA TACTTTTTGG ACAGAGCCTT TCTCCTCAAG
    GCTCTTGACG ACCGAGAAGC TGGGCCAGAG GAGAGCCGGG GCATGCCCTC CCGGGCTGCC
    GCCACTGAGG AGGGTACGCG AGCTGCCTCC CCCGCGCCCA GGAACCCAGA AGAGATTAGG
    AATTTGGTGA CCGGCAGGCC ATCACCTGAA AAGGAGGATG TAAAGCGTGT GGACAGCCCG
    GACGTTGACC CCCATGAACA CGACGGCCGG GACAGTCTTG ATTTGGATCA CTCTTTCGAT
    GTCTTCAAAA AACCTAAGCA TCCCCCAGCG TTCTTTGCTG CAGGCGGGCT CTCTGATGTG
    CTGAATGGCG GCGATGAAGT TTACGCCAAC TGTATGGTGA TTGACCAGGT TGGTGATTTG
    GATATTAACT ACATCAATTT AGAAGGAATC TCTACAGACG CCTGTGCTGA ATCCATGGGT
    TCTACTCTGG GGCCTCAGAA CTGCGAGCAT ATCCTTGCTG CTGAAACCAG CCATGGGAAA
    TACCCACTAA GTGAGAACAA GGAAGTGGTC TCAGATACCG AAGCTCAGCA GTCCTTCCCA
    TCACCGTCTA GTTCATATGC TTCCAATTTT GATCTTTCCT TTGGTCTTCA TGGATTTGAG
    AAGGAACAAA GTCTCCTGAA GAAAAGAAGC TCCAGCCTGG ATGCCTTGGA TGCCGACAGC
    GAAGGGGAAG GGCATTCTGA GCGGTCACAC ATCTGCTACA CGCCAGCATC TCAGAGTTCC
    TCGAGGACTG GGATTCCCAG TGGGGATGAG CTGGACTCCT TCGAGACCGC CACCAAACCT
    GATTTTAGTA TCTCCAGGAC AGAGTCGCTT TCTTTATCAA GTAGCCTGCA GTTGAAGGAA
    TCATTGCTTC CTGGAGTCCG CTCACGTTCT TATTCCTGCT CATCACCCAA AATTTCTTTA
    GGAAAAACTC GATTGGTGCG TGATTTCACA GTGTGCAATT CTGGTGAAGA GCAAAGAGCC
    TTCGGCTTCC CGGAGCCCGC CAGGGAGAGG AGGATTCAAG AGGAAGAATG GGATAAATAC
    ATCATACCTG CCAAGTCAGA GTCTGAGAAA TACAAAGTGA GCCGGACCTT CAGCTTCCTC
    ATGAACAGGA TGACAAGCCC TCGGAACAAA TCTAAGACGA AAAGCAAGGA TGCCAAAGAC
    AAAGAGAAAC CGAGCCGACA TCAGTTTGTC CCCGGAACCT TCTCTGGGGT TCTCCAGTGT
    TCCGTTTGTG ATAAAACGCT CCTGGGGAAG GAGTCATCCC AGTGTTCCAA CTGTAATGCA
    AACGTCCACA AGGGTTGTAA GGACGCTGCA CCTCCGTGTA CCAAGAAATT CCAGGAGAAA
    TATAACAAGA ACAAACCGCA GACCATCCTT GGAAACTCTT CATTTCGAGA CATCCCGCAA
    CCCAGTCTCT CCTTGCACCC TTCTTCCTCC ATGCCTATCG GATTGCCCAC TGGGAGAAAA
    GAAGCTTCTG CCCAGGCCCA TCCCTTGTCC AGAAGTGTTC CAGGCACTGC CTTGGAAAGC
    TTCAGAAGAT CGGGGCTATC CCTGGAGTCT GAGAGTGACG GGAACAGCTG GAGAAGCAGG
    TCTCATTCCG ATGAGCTGTT ACAGTCCATG GGCTCTTCTC CCTCCACGGA ATCCTTCTTA
    ATGGAAGATG TCGTGGACTC TTCTCTATGG AGTGACCTCA GCAGTGACGC CCAGGAGTTG
    GAAGCAGAAT CCTGGAGTCT GGTGGTGGAC CCCTCCTTTT GCAATAAGCA GGAGAAAGAT
    GTCATCAAAA GACAGGATGT CATTTTCGAG CTTATGCAAA CAGAGATGCA TCATATCCAG
    ACCCTGCTCA TCATGTCTGA GATCTTCAGG AAAGGAATGA AGGAGGAGCT GCAGCTTGAT
    CACAGCACCG TGGATAAAAT CTTTCCCTGC TTAGATGAGT TACTTGAAAT CCACAGGCAT
    TTCTTTTATA GTATGAAGGA GCGAAGGCAG GAGTCCTGTG CTGGTGGTGA CAGGAATTTT
    GTCATCAGCC GAATTGGAGA TATTCTAGTA CAGCAGTTTT CAGAAGAAAA TGCAAATCAA
    ATGAAGAAAA TATATGGAGA ATTCTGTAGC CATCACAAAG AAGCTGTTAG CCTCTATAAA
    GAACTCCAAC AGAATAAGAA GTTTCAGAAT TTTATTAAGC TTCGAAATAG TAATCTTTTG
    GCTCGACGCC GAGGAATCCC AGAATGCATT CTGCTAGTCA CTCAGCGCAT CACAAAATAC
    CCTGTCCTGG TGGAACGGAT TTTGCAGTAC ACAAAGGAAA GAAGTGAAGA ACATCAAGAC
    TTGTGCAAAG CTCTGTGCTT AATTAAAGAC ATGATTGCAG CAGTGGATTT AAAAGTCAGC
    GAGTATGAGA AAAACCAAAA ATGGCTTGAG ATCCTAAATA AGATCGAAAA CAAAACCTAC
    ACAAAGCTCA AAAATGGCCA CGTGTTTAGG AAGCAAGCAC TGATAAGTAA AGAAAGGACT
    CTCTTATATG ATGGCCTTGT TTATTGGAAA ACTGCTACGG GTCGTTTCAA AGATATCCTG
    GCTCTACTTC TAACTGATGT GCTGCTCTTT TTACAAGAAA AAGACCAGAA ATACATCTTT
    GCAGCTGTTG ATCAGAAGCC ATCGGTTATT TCCCTTCAGA AGCTCATTGC TAGAGAAGTC
    GCCAACGAGG AAAGAGGAAT GTTCCTGATC AGTGCATCCT CTGCTGGTCC TGAGATGTAC
    GAAATTCACA CCAACTCCAA GGAGGAACGA AATAACTGGA TGAGACGAAT CCAACAGGCC
    GTGGAAAGTT GCCCAGAAGA AGAAGGGGGA AGGACAAGTG AATCTGATGA AGAGAGGCGG
    AAAGCTGAAG CAAGAGTGGC CAAAATCCAG CAATGTCAAG AAATACTCAG TAACCAAGAC
    CAACAGATTT GCGCATATTT GGAGGAGAAG CTGCATATCT ACGCTGAACT TGGAGAACTG
    AGTGGATTCA AGGATGTCCA TCTAGAGCCC CACCTCCTCA TCAAACCTGA CCCAGGGGAG
    CCTCCCCAGG CAGCCTCATT ACTAGCAGCA GCCCTGAGAG AAGCGGAGAG CCTGCAGGTT
    GCCGTGAAGG CCTCACAAGC GGGTGATGCT CGCCCATCAC CTGAGGAGGG TTCTGCAGAA
    CCTGCGGTTG CAGATGCACA GGGTCCTCCC GAGGAGCCAG GATCATCTCC AGCCTCACTA
    GTCACAGAAG GGACAGGAGG AAGAGGCTGT TGGGATGTGG ATCCTGGCCC CCAGGGTGTG
    GGAACCGACT TGGCGGTCTC TGATGCAGGG GAGAAGGTGG AATATAGAAG TTTCACGGTT
    TCTTCACAGT CAGAGATTAT ACAAGCGATC CAGAATTTAA CCCGACTCCT CTATAGCCTT
    CAGGTGATGG AACTTAATCG ATCCGAGAGC TTGTGTCATG AAAATTCATT CTTCATTAAT
    GAAGCTTTAG TACAAATGTC ACTTAACACT TACAACAAAT CAACTCCATC AGGTGTCCAC
    CAGGAAGCCG CTTATCCCCA GAATATAGCT AATTCGGACT TGGTAAGGAC TAGTGAAAGT
    CCCGTAGACC TCCAGTTGGA CATTTCTCAG CCCTTGGATG TCAACCACGA ACTGTGGACA
    GCCGCTGGGT CCTGTCATCA GATACCCCCT CTCCACGAAA GCAGCAAGGA TTCTTGTAAA
    AATGGAAGAA TTTACATCAG TCAGCTATGT TTCCAGAGCC ATTCGTCAGA AATGTTTTGG
    CGACCAGACT TGACCATCTC AGCTTGGGAT AGAGAGCTTC CGAATCTGCA GAACTTCCAA
    CCGCTGTTGG TTGCATTATG TAGATGGTTG CGTTACGTCC GTACATCCCC GGGAGATCTT
    GCCGTCATCC TCGAGTCCCC TGAGCAGGTT TTTCAGCTAG AAAGAAAATG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35385
    Clone ID Related Accession (Same CDS sequence) XM_014109078.2 , XM_014109078.1
    Accession Version XM_014109078.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4893bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rho guanine nucleotide exchange factor 28 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109078.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGTTGA GCTGCAGCGA AGTACCTCTT TATGGGCAGA TGACCGTCTA TGCAAAGTTT 
    GGGAAAAATG TTTATCTTCC TAAAGATGCC GAGTTTTACT TTATTTATAA TGGATCTCAT
    CAGAGACACG TCGTGATTGC AAAACGCACT GAGGATAATG TTCTCCAGTC CAGCATTCCA
    GGGCATGGGC TTCAGGAGAC GGTGACGGTG TCTGTATGCC TCTGCTCAGA AGGTTACTCC
    CCAGTGACCA TGGGCTCCGG CTCAGTGACC TACGTGGACA ACATGGCTTG CAGGCTGGCT
    CGTTTGCTGG TCACCCAGGC TGATCGCCTC ACAGCCAGTA GTCATCAGAC CTTTCTGACT
    CCATTTGCCC TGACGGTGGG AGCATTGCCT GCTTTGGACG AGGAGCTGGT GCTGGCTCTG
    ACCCATCTGG AGTTGCCTCT TGAGTGGAGT GTCTTGGGAA ATTCTTCACT TGAAGTTTCT
    TTATGCAGAG AGTCTCTTCT ACACCTGGCT ATGAGATGGG GCCTGCCTAA GCTCTCTCAT
    TTCCTCTTGT GCCTCCCTGG GGGAGTTCAG GCTTTGTCTT TACCCAATGA AGAAGGTGCC
    ACACCATTAG ACTTGGCTTT ACGTGCCGGA CACTCCAAGC TGGTTGAAGA CATCACAAAT
    TTCCAGGGCA GACGTTCCCC CGACCTCTCC CGAGTGCACC TCGGCGGACG TGCCTTCCTG
    CAGTATGTTC ACCCGTCGGG GACACTGACG CTGACGCTGA ACCACACAGC TGAGCATTTG
    TTGGAGGCAG ATATTAAACT CTTCAGGAAA TACTTTTTGG ACAGAGCCTT TCTCCTCAAG
    GCTCTTGACG ACCGAGAAGC TGGGCCAGAG GAGAGCCGGG GCATGCCCTC CCGGGCTGCC
    GCCACTGAGG AGGGTACGCG AGCTGCCTCC CCCGCGCCCA GGAACCCAGA AGAGATTAGG
    AATTTGGTGA CCGGCAGGCC ATCACCTGAA AAGGAGGATG TAAAGCGTGT GGACAGCCCG
    GACGTTGACC CCCATGAACA CGACGGCCGG GACAGTCTTG ATTTGGATCA CTCTTTCGAT
    GTCTTCAAAA AACCTAAGCA TCCCCCAGCG TTCTTTGCTG CAGGCGGGCT CTCTGATGTG
    CTGAATGGCG GCGATGAAGT TTACGCCAAC TGTATGGTGA TTGACCAGGT TGGTGATTTG
    GATATTAACT ACATCAATTT AGAAGGAATC TCTACAGACG CCTGTGCTGA ATCCATGGGT
    TCTACTCTGG GGCCTCAGAA CTGCGAGCAT ATCCTTGCTG CTGAAACCAG CCATGGGAAA
    TACCCACTAA GTGAGAACAA GGAAGTGGTC TCAGATACCG AAGCTCAGCA GTCCTTCCCA
    TCACCGTCTA GTTCATATGC TTCCAATTTT GATCTTTCCT TTGGTCTTCA TGGATTTGAG
    AAGGAACAAA GTCTCCTGAA GAAAAGAAGC TCCAGCCTGG ATGCCTTGGA TGCCGACAGC
    GAAGGGGAAG GGCATTCTGA GCGGTCACAC ATCTGCTACA CGCCAGCATC TCAGAGTTCC
    TCGAGGACTG GGATTCCCAG TGGGGATGAG CTGGACTCCT TCGAGACCGC CACCAAACCT
    GATTTTAGTA TCTCCAGGAC AGAGTCGCTT TCTTTATCAA GTAGCCTGCA GTTGAAGGAA
    TCATTGCTTC CTGGAGTCCG CTCACGTTCT TATTCCTGCT CATCACCCAA AATTTCTTTA
    GGAAAAACTC GATTGGTGCG TGATTTCACA GTGTGCAATT CTGGTGAAGA GCAAAGAGCC
    TTCGGCTTCC CGGAGCCCGC CAGGGAGAGG AGGATTCAAG AGGAAGAATG GGATAAATAC
    ATCATACCTG CCAAGTCAGA GTCTGAGAAA TACAAAGTGA GCCGGACCTT CAGCTTCCTC
    ATGAACAGGA TGACAAGCCC TCGGAACAAA TCTAAGACGA AAAGCAAGGA TGCCAAAGAC
    AAAGAGAAAC CGAGCCGACA TCAGTTTGTC CCCGGAACCT TCTCTGGGGT TCTCCAGTGT
    TCCGTTTGTG ATAAAACGCT CCTGGGGAAG GAGTCATCCC AGTGTTCCAA CTGTAATGCA
    AACGTCCACA AGGGTTGTAA GGACGCTGCA CCTCCGTGTA CCAAGAAATT CCAGGAGAAA
    TATAACAAGA ACAAACCGCA GACCATCCTT GGAAACTCTT CATTTCGAGA CATCCCGCAA
    CCCAGTCTCT CCTTGCACCC TTCTTCCTCC ATGCCTATCG GATTGCCCAC TGGGAGAAAA
    GAAGCTTCTG CCCAGGCCCA TCCCTTGTCC AGAAGTGTTC CAGGCACTGC CTTGGAAAGC
    TTCAGAAGAT CGGGGCTATC CCTGGAGTCT GAGAGTGACG GGAACAGCTG GAGAAGCAGG
    TCTCATTCCG ATGAGCTGTT ACAGTCCATG GGCTCTTCTC CCTCCACGGA ATCCTTCTTA
    ATGGAAGATG TCGTGGACTC TTCTCTATGG AGTGACCTCA GCAGTGACGC CCAGGAGTTG
    GAAGCAGAAT CCTGGAGTCT GGTGGTGGAC CCCTCCTTTT GCAATAAGCA GGAGAAAGAT
    GTCATCAAAA GACAGGATGT CATTTTCGAG CTTATGCAAA CAGAGATGCA TCATATCCAG
    ACCCTGCTCA TCATGTCTGA GATCTTCAGG AAAGGAATGA AGGAGGAGCT GCAGCTTGAT
    CACAGCACCG TGGATAAAAT CTTTCCCTGC TTAGATGAGT TACTTGAAAT CCACAGGCAT
    TTCTTTTATA GTATGAAGGA GCGAAGGCAG GAGTCCTGTG CTGGTGGTGA CAGGAATTTT
    GTCATCAGCC GAATTGGAGA TATTCTAGTA CAGCAGTTTT CAGAAGAAAA TGCAAATCAA
    ATGAAGAAAA TATATGGAGA ATTCTGTAGC CATCACAAAG AAGCTGTTAG CCTCTATAAA
    GAACTCCAAC AGAATAAGAA GTTTCAGAAT TTTATTAAGC TTCGAAATAG TAATCTTTTG
    GCTCGACGCC GAGGAATCCC AGAATGCATT CTGCTAGTCA CTCAGCGCAT CACAAAATAC
    CCTGTCCTGG TGGAACGGAT TTTGCAGTAC ACAAAGGAAA GAAGTGAAGA ACATCAAGAC
    TTGTGCAAAG CTCTGTGCTT AATTAAAGAC ATGATTGCAG CAGTGGATTT AAAAGTCAGC
    GAGTATGAGA AAAACCAAAA ATGGCTTGAG ATCCTAAATA AGATCGAAAA CAAAACCTAC
    ACAAAGCTCA AAAATGGCCA CGTGTTTAGG AAGCAAGCAC TGATAAGTAA AGAAAGGACT
    CTCTTATATG ATGGCCTTGT TTATTGGAAA ACTGCTACGG GTCGTTTCAA AGATATCCTG
    GCTCTACTTC TAACTGATGT GCTGCTCTTT TTACAAGAAA AAGACCAGAA ATACATCTTT
    GCAGCTGTTG ATCAGAAGCC ATCGGTTATT TCCCTTCAGA AGCTCATTGC TAGAGAAGTC
    GCCAACGAGG AAAGAGGAAT GTTCCTGATC AGTGCATCCT CTGCTGGTCC TGAGATGTAC
    GAAATTCACA CCAACTCCAA GGAGGAACGA AATAACTGGA TGAGACGAAT CCAACAGGCC
    GTGGAAAGTT GCCCAGAAGA AGAAGGGGGA AGGACAAGTG AATCTGATGA AGAGAGGCGG
    AAAGCTGAAG CAAGAGTGGC CAAAATCCAG CAATGTCAAG AAATACTCAG TAACCAAGAC
    CAACAGATTT GCGCATATTT GGAGGAGAAG CTGCATATCT ACGCTGAACT TGGAGAACTG
    AGTGGATTCA AGGATGTCCA TCTAGAGCCC CACCTCCTCA TCAAACCTGA CCCAGGGGAG
    CCTCCCCAGG CAGCCTCATT ACTAGCAGCA GCCCTGAGAG AAGCGGAGAG CCTGCAGGTT
    GCCGTGAAGG CCTCACAAGC GGGTGATGCT CGCCCATCAC CTGAGGAGGG TTCTGCAGAA
    CCTGCGGTTG CAGATGCACA GGGTCCTCCC GAGGAGCCAG GATCATCTCC AGCCTCACTA
    GTCACAGAAG GGACAGGAGG AAGAGGCTGT TGGGATGTGG ATCCTGGCCC CCAGGGTGTG
    GGAACCGACT TGGCGGTCTC TGATGCAGGG GAGAAGAGTC TTAAAAATCT GTGCTGCAGC
    CGGCAAAGTC CCTGGGGTGT CAGTCACCAT CACCAGCTGC AGCCCTGCAC AGGGACCACA
    AGGGGGAGGG CCAGCTCCAC GAAAAGTAGG ACACTACACA GCTCAGGCTT GTATCAGGCT
    TCGTGGTTCT TGGGAGTGGA ATATAGAAGT TTCACGGTTT CTTCACAGTC AGAGATTATA
    CAAGCGATCC AGAATTTAAC CCGACTCCTC TATAGCCTTC AGGTGATGGA ACTTAATCGA
    TCCGAGAGCT TGTGTCATGA AAATTCATTC TTCATTAATG AAGCTTTAGT ACAAATGTCA
    CTTAACACTT ACAACAAATC AACTCCATCA GGTGTCCACC AGGAAGCCGC TTATCCCCAG
    AATATAGCTA ATTCGGACTT GGTAAGGACT AGTGAAAGTC CCGTAGACCT CCAGTTGGAC
    ATTTCTCAGC CCTTGGATGT CAACCACGAA CTGTGGACAG CCGCTGGGTC CTGTCATCAG
    ATACCCCCTC TCCACGAAAG CAGCAAGGAT TCTTGTAAAA ATGGAAGAAT TTACATCAGT
    CAGCTATGTT TCCAGAGCCA TTCGTCAGAA ATGTTTTGGC GACCAGACTT GACCATCTCA
    GCTTGGGATA GAGAGCTTCC GAATCTGCAG AACTTCCAAC CGCTGTTGGT TGCATTATGT
    AGATGGTTGC GTTACGTCCG TACATCCCCG GGAGATCTTG CCGTCATCCT CGAGTCCCCT
    GAGCAGGTTT TTCAGCTAGA AAGAAAATGT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013964553.1
    CDS92..4984
    Translation

    Target ORF information:

    RefSeq Version XM_014109078.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109078.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGTTGA GCTGCAGCGA AGTACCTCTT TATGGGCAGA TGACCGTCTA TGCAAAGTTT 
    GGGAAAAATG TTTATCTTCC TAAAGATGCC GAGTTTTACT TTATTTATAA TGGATCTCAT
    CAGAGACACG TCGTGATTGC AAAACGCACT GAGGATAATG TTCTCCAGTC CAGCATTCCA
    GGGCATGGGC TTCAGGAGAC GGTGACGGTG TCTGTATGCC TCTGCTCAGA AGGTTACTCC
    CCAGTGACCA TGGGCTCCGG CTCAGTGACC TACGTGGACA ACATGGCTTG CAGGCTGGCT
    CGTTTGCTGG TCACCCAGGC TGATCGCCTC ACAGCCAGTA GTCATCAGAC CTTTCTGACT
    CCATTTGCCC TGACGGTGGG AGCATTGCCT GCTTTGGACG AGGAGCTGGT GCTGGCTCTG
    ACCCATCTGG AGTTGCCTCT TGAGTGGAGT GTCTTGGGAA ATTCTTCACT TGAAGTTTCT
    TTATGCAGAG AGTCTCTTCT ACACCTGGCT ATGAGATGGG GCCTGCCTAA GCTCTCTCAT
    TTCCTCTTGT GCCTCCCTGG GGGAGTTCAG GCTTTGTCTT TACCCAATGA AGAAGGTGCC
    ACACCATTAG ACTTGGCTTT ACGTGCCGGA CACTCCAAGC TGGTTGAAGA CATCACAAAT
    TTCCAGGGCA GACGTTCCCC CGACCTCTCC CGAGTGCACC TCGGCGGACG TGCCTTCCTG
    CAGTATGTTC ACCCGTCGGG GACACTGACG CTGACGCTGA ACCACACAGC TGAGCATTTG
    TTGGAGGCAG ATATTAAACT CTTCAGGAAA TACTTTTTGG ACAGAGCCTT TCTCCTCAAG
    GCTCTTGACG ACCGAGAAGC TGGGCCAGAG GAGAGCCGGG GCATGCCCTC CCGGGCTGCC
    GCCACTGAGG AGGGTACGCG AGCTGCCTCC CCCGCGCCCA GGAACCCAGA AGAGATTAGG
    AATTTGGTGA CCGGCAGGCC ATCACCTGAA AAGGAGGATG TAAAGCGTGT GGACAGCCCG
    GACGTTGACC CCCATGAACA CGACGGCCGG GACAGTCTTG ATTTGGATCA CTCTTTCGAT
    GTCTTCAAAA AACCTAAGCA TCCCCCAGCG TTCTTTGCTG CAGGCGGGCT CTCTGATGTG
    CTGAATGGCG GCGATGAAGT TTACGCCAAC TGTATGGTGA TTGACCAGGT TGGTGATTTG
    GATATTAACT ACATCAATTT AGAAGGAATC TCTACAGACG CCTGTGCTGA ATCCATGGGT
    TCTACTCTGG GGCCTCAGAA CTGCGAGCAT ATCCTTGCTG CTGAAACCAG CCATGGGAAA
    TACCCACTAA GTGAGAACAA GGAAGTGGTC TCAGATACCG AAGCTCAGCA GTCCTTCCCA
    TCACCGTCTA GTTCATATGC TTCCAATTTT GATCTTTCCT TTGGTCTTCA TGGATTTGAG
    AAGGAACAAA GTCTCCTGAA GAAAAGAAGC TCCAGCCTGG ATGCCTTGGA TGCCGACAGC
    GAAGGGGAAG GGCATTCTGA GCGGTCACAC ATCTGCTACA CGCCAGCATC TCAGAGTTCC
    TCGAGGACTG GGATTCCCAG TGGGGATGAG CTGGACTCCT TCGAGACCGC CACCAAACCT
    GATTTTAGTA TCTCCAGGAC AGAGTCGCTT TCTTTATCAA GTAGCCTGCA GTTGAAGGAA
    TCATTGCTTC CTGGAGTCCG CTCACGTTCT TATTCCTGCT CATCACCCAA AATTTCTTTA
    GGAAAAACTC GATTGGTGCG TGATTTCACA GTGTGCAATT CTGGTGAAGA GCAAAGAGCC
    TTCGGCTTCC CGGAGCCCGC CAGGGAGAGG AGGATTCAAG AGGAAGAATG GGATAAATAC
    ATCATACCTG CCAAGTCAGA GTCTGAGAAA TACAAAGTGA GCCGGACCTT CAGCTTCCTC
    ATGAACAGGA TGACAAGCCC TCGGAACAAA TCTAAGACGA AAAGCAAGGA TGCCAAAGAC
    AAAGAGAAAC CGAGCCGACA TCAGTTTGTC CCCGGAACCT TCTCTGGGGT TCTCCAGTGT
    TCCGTTTGTG ATAAAACGCT CCTGGGGAAG GAGTCATCCC AGTGTTCCAA CTGTAATGCA
    AACGTCCACA AGGGTTGTAA GGACGCTGCA CCTCCGTGTA CCAAGAAATT CCAGGAGAAA
    TATAACAAGA ACAAACCGCA GACCATCCTT GGAAACTCTT CATTTCGAGA CATCCCGCAA
    CCCAGTCTCT CCTTGCACCC TTCTTCCTCC ATGCCTATCG GATTGCCCAC TGGGAGAAAA
    GAAGCTTCTG CCCAGGCCCA TCCCTTGTCC AGAAGTGTTC CAGGCACTGC CTTGGAAAGC
    TTCAGAAGAT CGGGGCTATC CCTGGAGTCT GAGAGTGACG GGAACAGCTG GAGAAGCAGG
    TCTCATTCCG ATGAGCTGTT ACAGTCCATG GGCTCTTCTC CCTCCACGGA ATCCTTCTTA
    ATGGAAGATG TCGTGGACTC TTCTCTATGG AGTGACCTCA GCAGTGACGC CCAGGAGTTG
    GAAGCAGAAT CCTGGAGTCT GGTGGTGGAC CCCTCCTTTT GCAATAAGCA GGAGAAAGAT
    GTCATCAAAA GACAGGATGT CATTTTCGAG CTTATGCAAA CAGAGATGCA TCATATCCAG
    ACCCTGCTCA TCATGTCTGA GATCTTCAGG AAAGGAATGA AGGAGGAGCT GCAGCTTGAT
    CACAGCACCG TGGATAAAAT CTTTCCCTGC TTAGATGAGT TACTTGAAAT CCACAGGCAT
    TTCTTTTATA GTATGAAGGA GCGAAGGCAG GAGTCCTGTG CTGGTGGTGA CAGGAATTTT
    GTCATCAGCC GAATTGGAGA TATTCTAGTA CAGCAGTTTT CAGAAGAAAA TGCAAATCAA
    ATGAAGAAAA TATATGGAGA ATTCTGTAGC CATCACAAAG AAGCTGTTAG CCTCTATAAA
    GAACTCCAAC AGAATAAGAA GTTTCAGAAT TTTATTAAGC TTCGAAATAG TAATCTTTTG
    GCTCGACGCC GAGGAATCCC AGAATGCATT CTGCTAGTCA CTCAGCGCAT CACAAAATAC
    CCTGTCCTGG TGGAACGGAT TTTGCAGTAC ACAAAGGAAA GAAGTGAAGA ACATCAAGAC
    TTGTGCAAAG CTCTGTGCTT AATTAAAGAC ATGATTGCAG CAGTGGATTT AAAAGTCAGC
    GAGTATGAGA AAAACCAAAA ATGGCTTGAG ATCCTAAATA AGATCGAAAA CAAAACCTAC
    ACAAAGCTCA AAAATGGCCA CGTGTTTAGG AAGCAAGCAC TGATAAGTAA AGAAAGGACT
    CTCTTATATG ATGGCCTTGT TTATTGGAAA ACTGCTACGG GTCGTTTCAA AGATATCCTG
    GCTCTACTTC TAACTGATGT GCTGCTCTTT TTACAAGAAA AAGACCAGAA ATACATCTTT
    GCAGCTGTTG ATCAGAAGCC ATCGGTTATT TCCCTTCAGA AGCTCATTGC TAGAGAAGTC
    GCCAACGAGG AAAGAGGAAT GTTCCTGATC AGTGCATCCT CTGCTGGTCC TGAGATGTAC
    GAAATTCACA CCAACTCCAA GGAGGAACGA AATAACTGGA TGAGACGAAT CCAACAGGCC
    GTGGAAAGTT GCCCAGAAGA AGAAGGGGGA AGGACAAGTG AATCTGATGA AGAGAGGCGG
    AAAGCTGAAG CAAGAGTGGC CAAAATCCAG CAATGTCAAG AAATACTCAG TAACCAAGAC
    CAACAGATTT GCGCATATTT GGAGGAGAAG CTGCATATCT ACGCTGAACT TGGAGAACTG
    AGTGGATTCA AGGATGTCCA TCTAGAGCCC CACCTCCTCA TCAAACCTGA CCCAGGGGAG
    CCTCCCCAGG CAGCCTCATT ACTAGCAGCA GCCCTGAGAG AAGCGGAGAG CCTGCAGGTT
    GCCGTGAAGG CCTCACAAGC GGGTGATGCT CGCCCATCAC CTGAGGAGGG TTCTGCAGAA
    CCTGCGGTTG CAGATGCACA GGGTCCTCCC GAGGAGCCAG GATCATCTCC AGCCTCACTA
    GTCACAGAAG GGACAGGAGG AAGAGGCTGT TGGGATGTGG ATCCTGGCCC CCAGGGTGTG
    GGAACCGACT TGGCGGTCTC TGATGCAGGG GAGAAGAGTC TTAAAAATCT GTGCTGCAGC
    CGGCAAAGTC CCTGGGGTGT CAGTCACCAT CACCAGCTGC AGCCCTGCAC AGGGACCACA
    AGGGGGAGGG CCAGCTCCAC GAAAAGTAGG ACACTACACA GCTCAGGCTT GTATCAGGCT
    TCGTGGTTCT TGGGAGTGGA ATATAGAAGT TTCACGGTTT CTTCACAGTC AGAGATTATA
    CAAGCGATCC AGAATTTAAC CCGACTCCTC TATAGCCTTC AGGTGATGGA ACTTAATCGA
    TCCGAGAGCT TGTGTCATGA AAATTCATTC TTCATTAATG AAGCTTTAGT ACAAATGTCA
    CTTAACACTT ACAACAAATC AACTCCATCA GGTGTCCACC AGGAAGCCGC TTATCCCCAG
    AATATAGCTA ATTCGGACTT GGTAAGGACT AGTGAAAGTC CCGTAGACCT CCAGTTGGAC
    ATTTCTCAGC CCTTGGATGT CAACCACGAA CTGTGGACAG CCGCTGGGTC CTGTCATCAG
    ATACCCCCTC TCCACGAAAG CAGCAAGGAT TCTTGTAAAA ATGGAAGAAT TTACATCAGT
    CAGCTATGTT TCCAGAGCCA TTCGTCAGAA ATGTTTTGGC GACCAGACTT GACCATCTCA
    GCTTGGGATA GAGAGCTTCC GAATCTGCAG AACTTCCAAC CGCTGTTGGT TGCATTATGT
    AGATGGTTGC GTTACGTCCG TACATCCCCG GGAGATCTTG CCGTCATCCT CGAGTCCCCT
    GAGCAGGTTT TTCAGCTAGA AAGAAAATGT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35385
    Clone ID Related Accession (Same CDS sequence) XM_014109078.2 , XM_014109078.1
    Accession Version XM_014109078.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4893bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product rho guanine nucleotide exchange factor 28 isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGTTGA GCTGCAGCGA AGTACCTCTT TATGGGCAGA TGACCGTCTA TGCAAAGTTT 
    GGGAAAAATG TTTATCTTCC TAAAGATGCC GAGTTTTACT TTATTTATAA TGGATCTCAT
    CAGAGACACG TCGTGATTGC AAAACGCACT GAGGATAATG TTCTCCAGTC CAGCATTCCA
    GGGCATGGGC TTCAGGAGAC GGTGACGGTG TCTGTATGCC TCTGCTCAGA AGGTTACTCC
    CCAGTGACCA TGGGCTCCGG CTCAGTGACC TACGTGGACA ACATGGCTTG CAGGCTGGCT
    CGTTTGCTGG TCACCCAGGC TGATCGCCTC ACAGCCAGTA GTCATCAGAC CTTTCTGACT
    CCATTTGCCC TGACGGTGGG AGCATTGCCT GCTTTGGACG AGGAGCTGGT GCTGGCTCTG
    ACCCATCTGG AGTTGCCTCT TGAGTGGAGT GTCTTGGGAA ATTCTTCACT TGAAGTTTCT
    TTATGCAGAG AGTCTCTTCT ACACCTGGCT ATGAGATGGG GCCTGCCTAA GCTCTCTCAT
    TTCCTCTTGT GCCTCCCTGG GGGAGTTCAG GCTTTGTCTT TACCCAATGA AGAAGGTGCC
    ACACCATTAG ACTTGGCTTT ACGTGCCGGA CACTCCAAGC TGGTTGAAGA CATCACAAAT
    TTCCAGGGCA GACGTTCCCC CGACCTCTCC CGAGTGCACC TCGGCGGACG TGCCTTCCTG
    CAGTATGTTC ACCCGTCGGG GACACTGACG CTGACGCTGA ACCACACAGC TGAGCATTTG
    TTGGAGGCAG ATATTAAACT CTTCAGGAAA TACTTTTTGG ACAGAGCCTT TCTCCTCAAG
    GCTCTTGACG ACCGAGAAGC TGGGCCAGAG GAGAGCCGGG GCATGCCCTC CCGGGCTGCC
    GCCACTGAGG AGGGTACGCG AGCTGCCTCC CCCGCGCCCA GGAACCCAGA AGAGATTAGG
    AATTTGGTGA CCGGCAGGCC ATCACCTGAA AAGGAGGATG TAAAGCGTGT GGACAGCCCG
    GACGTTGACC CCCATGAACA CGACGGCCGG GACAGTCTTG ATTTGGATCA CTCTTTCGAT
    GTCTTCAAAA AACCTAAGCA TCCCCCAGCG TTCTTTGCTG CAGGCGGGCT CTCTGATGTG
    CTGAATGGCG GCGATGAAGT TTACGCCAAC TGTATGGTGA TTGACCAGGT TGGTGATTTG
    GATATTAACT ACATCAATTT AGAAGGAATC TCTACAGACG CCTGTGCTGA ATCCATGGGT
    TCTACTCTGG GGCCTCAGAA CTGCGAGCAT ATCCTTGCTG CTGAAACCAG CCATGGGAAA
    TACCCACTAA GTGAGAACAA GGAAGTGGTC TCAGATACCG AAGCTCAGCA GTCCTTCCCA
    TCACCGTCTA GTTCATATGC TTCCAATTTT GATCTTTCCT TTGGTCTTCA TGGATTTGAG
    AAGGAACAAA GTCTCCTGAA GAAAAGAAGC TCCAGCCTGG ATGCCTTGGA TGCCGACAGC
    GAAGGGGAAG GGCATTCTGA GCGGTCACAC ATCTGCTACA CGCCAGCATC TCAGAGTTCC
    TCGAGGACTG GGATTCCCAG TGGGGATGAG CTGGACTCCT TCGAGACCGC CACCAAACCT
    GATTTTAGTA TCTCCAGGAC AGAGTCGCTT TCTTTATCAA GTAGCCTGCA GTTGAAGGAA
    TCATTGCTTC CTGGAGTCCG CTCACGTTCT TATTCCTGCT CATCACCCAA AATTTCTTTA
    GGAAAAACTC GATTGGTGCG TGATTTCACA GTGTGCAATT CTGGTGAAGA GCAAAGAGCC
    TTCGGCTTCC CGGAGCCCGC CAGGGAGAGG AGGATTCAAG AGGAAGAATG GGATAAATAC
    ATCATACCTG CCAAGTCAGA GTCTGAGAAA TACAAAGTGA GCCGGACCTT CAGCTTCCTC
    ATGAACAGGA TGACAAGCCC TCGGAACAAA TCTAAGACGA AAAGCAAGGA TGCCAAAGAC
    AAAGAGAAAC CGAGCCGACA TCAGTTTGTC CCCGGAACCT TCTCTGGGGT TCTCCAGTGT
    TCCGTTTGTG ATAAAACGCT CCTGGGGAAG GAGTCATCCC AGTGTTCCAA CTGTAATGCA
    AACGTCCACA AGGGTTGTAA GGACGCTGCA CCTCCGTGTA CCAAGAAATT CCAGGAGAAA
    TATAACAAGA ACAAACCGCA GACCATCCTT GGAAACTCTT CATTTCGAGA CATCCCGCAA
    CCCAGTCTCT CCTTGCACCC TTCTTCCTCC ATGCCTATCG GATTGCCCAC TGGGAGAAAA
    GAAGCTTCTG CCCAGGCCCA TCCCTTGTCC AGAAGTGTTC CAGGCACTGC CTTGGAAAGC
    TTCAGAAGAT CGGGGCTATC CCTGGAGTCT GAGAGTGACG GGAACAGCTG GAGAAGCAGG
    TCTCATTCCG ATGAGCTGTT ACAGTCCATG GGCTCTTCTC CCTCCACGGA ATCCTTCTTA
    ATGGAAGATG TCGTGGACTC TTCTCTATGG AGTGACCTCA GCAGTGACGC CCAGGAGTTG
    GAAGCAGAAT CCTGGAGTCT GGTGGTGGAC CCCTCCTTTT GCAATAAGCA GGAGAAAGAT
    GTCATCAAAA GACAGGATGT CATTTTCGAG CTTATGCAAA CAGAGATGCA TCATATCCAG
    ACCCTGCTCA TCATGTCTGA GATCTTCAGG AAAGGAATGA AGGAGGAGCT GCAGCTTGAT
    CACAGCACCG TGGATAAAAT CTTTCCCTGC TTAGATGAGT TACTTGAAAT CCACAGGCAT
    TTCTTTTATA GTATGAAGGA GCGAAGGCAG GAGTCCTGTG CTGGTGGTGA CAGGAATTTT
    GTCATCAGCC GAATTGGAGA TATTCTAGTA CAGCAGTTTT CAGAAGAAAA TGCAAATCAA
    ATGAAGAAAA TATATGGAGA ATTCTGTAGC CATCACAAAG AAGCTGTTAG CCTCTATAAA
    GAACTCCAAC AGAATAAGAA GTTTCAGAAT TTTATTAAGC TTCGAAATAG TAATCTTTTG
    GCTCGACGCC GAGGAATCCC AGAATGCATT CTGCTAGTCA CTCAGCGCAT CACAAAATAC
    CCTGTCCTGG TGGAACGGAT TTTGCAGTAC ACAAAGGAAA GAAGTGAAGA ACATCAAGAC
    TTGTGCAAAG CTCTGTGCTT AATTAAAGAC ATGATTGCAG CAGTGGATTT AAAAGTCAGC
    GAGTATGAGA AAAACCAAAA ATGGCTTGAG ATCCTAAATA AGATCGAAAA CAAAACCTAC
    ACAAAGCTCA AAAATGGCCA CGTGTTTAGG AAGCAAGCAC TGATAAGTAA AGAAAGGACT
    CTCTTATATG ATGGCCTTGT TTATTGGAAA ACTGCTACGG GTCGTTTCAA AGATATCCTG
    GCTCTACTTC TAACTGATGT GCTGCTCTTT TTACAAGAAA AAGACCAGAA ATACATCTTT
    GCAGCTGTTG ATCAGAAGCC ATCGGTTATT TCCCTTCAGA AGCTCATTGC TAGAGAAGTC
    GCCAACGAGG AAAGAGGAAT GTTCCTGATC AGTGCATCCT CTGCTGGTCC TGAGATGTAC
    GAAATTCACA CCAACTCCAA GGAGGAACGA AATAACTGGA TGAGACGAAT CCAACAGGCC
    GTGGAAAGTT GCCCAGAAGA AGAAGGGGGA AGGACAAGTG AATCTGATGA AGAGAGGCGG
    AAAGCTGAAG CAAGAGTGGC CAAAATCCAG CAATGTCAAG AAATACTCAG TAACCAAGAC
    CAACAGATTT GCGCATATTT GGAGGAGAAG CTGCATATCT ACGCTGAACT TGGAGAACTG
    AGTGGATTCA AGGATGTCCA TCTAGAGCCC CACCTCCTCA TCAAACCTGA CCCAGGGGAG
    CCTCCCCAGG CAGCCTCATT ACTAGCAGCA GCCCTGAGAG AAGCGGAGAG CCTGCAGGTT
    GCCGTGAAGG CCTCACAAGC GGGTGATGCT CGCCCATCAC CTGAGGAGGG TTCTGCAGAA
    CCTGCGGTTG CAGATGCACA GGGTCCTCCC GAGGAGCCAG GATCATCTCC AGCCTCACTA
    GTCACAGAAG GGACAGGAGG AAGAGGCTGT TGGGATGTGG ATCCTGGCCC CCAGGGTGTG
    GGAACCGACT TGGCGGTCTC TGATGCAGGG GAGAAGAGTC TTAAAAATCT GTGCTGCAGC
    CGGCAAAGTC CCTGGGGTGT CAGTCACCAT CACCAGCTGC AGCCCTGCAC AGGGACCACA
    AGGGGGAGGG CCAGCTCCAC GAAAAGTAGG ACACTACACA GCTCAGGCTT GTATCAGGCT
    TCGTGGTTCT TGGGAGTGGA ATATAGAAGT TTCACGGTTT CTTCACAGTC AGAGATTATA
    CAAGCGATCC AGAATTTAAC CCGACTCCTC TATAGCCTTC AGGTGATGGA ACTTAATCGA
    TCCGAGAGCT TGTGTCATGA AAATTCATTC TTCATTAATG AAGCTTTAGT ACAAATGTCA
    CTTAACACTT ACAACAAATC AACTCCATCA GGTGTCCACC AGGAAGCCGC TTATCCCCAG
    AATATAGCTA ATTCGGACTT GGTAAGGACT AGTGAAAGTC CCGTAGACCT CCAGTTGGAC
    ATTTCTCAGC CCTTGGATGT CAACCACGAA CTGTGGACAG CCGCTGGGTC CTGTCATCAG
    ATACCCCCTC TCCACGAAAG CAGCAAGGAT TCTTGTAAAA ATGGAAGAAT TTACATCAGT
    CAGCTATGTT TCCAGAGCCA TTCGTCAGAA ATGTTTTGGC GACCAGACTT GACCATCTCA
    GCTTGGGATA GAGAGCTTCC GAATCTGCAG AACTTCCAAC CGCTGTTGGT TGCATTATGT
    AGATGGTTGC GTTACGTCCG TACATCCCCG GGAGATCTTG CCGTCATCCT CGAGTCCCCT
    GAGCAGGTTT TTCAGCTAGA AAGAAAATGT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013964553.1
    CDS12..4904
    Translation

    Target ORF information:

    RefSeq Version XM_014109078.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 28 (ARHGEF28), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109078.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGTTGA GCTGCAGCGA AGTACCTCTT TATGGGCAGA TGACCGTCTA TGCAAAGTTT 
    GGGAAAAATG TTTATCTTCC TAAAGATGCC GAGTTTTACT TTATTTATAA TGGATCTCAT
    CAGAGACACG TCGTGATTGC AAAACGCACT GAGGATAATG TTCTCCAGTC CAGCATTCCA
    GGGCATGGGC TTCAGGAGAC GGTGACGGTG TCTGTATGCC TCTGCTCAGA AGGTTACTCC
    CCAGTGACCA TGGGCTCCGG CTCAGTGACC TACGTGGACA ACATGGCTTG CAGGCTGGCT
    CGTTTGCTGG TCACCCAGGC TGATCGCCTC ACAGCCAGTA GTCATCAGAC CTTTCTGACT
    CCATTTGCCC TGACGGTGGG AGCATTGCCT GCTTTGGACG AGGAGCTGGT GCTGGCTCTG
    ACCCATCTGG AGTTGCCTCT TGAGTGGAGT GTCTTGGGAA ATTCTTCACT TGAAGTTTCT
    TTATGCAGAG AGTCTCTTCT ACACCTGGCT ATGAGATGGG GCCTGCCTAA GCTCTCTCAT
    TTCCTCTTGT GCCTCCCTGG GGGAGTTCAG GCTTTGTCTT TACCCAATGA AGAAGGTGCC
    ACACCATTAG ACTTGGCTTT ACGTGCCGGA CACTCCAAGC TGGTTGAAGA CATCACAAAT
    TTCCAGGGCA GACGTTCCCC CGACCTCTCC CGAGTGCACC TCGGCGGACG TGCCTTCCTG
    CAGTATGTTC ACCCGTCGGG GACACTGACG CTGACGCTGA ACCACACAGC TGAGCATTTG
    TTGGAGGCAG ATATTAAACT CTTCAGGAAA TACTTTTTGG ACAGAGCCTT TCTCCTCAAG
    GCTCTTGACG ACCGAGAAGC TGGGCCAGAG GAGAGCCGGG GCATGCCCTC CCGGGCTGCC
    GCCACTGAGG AGGGTACGCG AGCTGCCTCC CCCGCGCCCA GGAACCCAGA AGAGATTAGG
    AATTTGGTGA CCGGCAGGCC ATCACCTGAA AAGGAGGATG TAAAGCGTGT GGACAGCCCG
    GACGTTGACC CCCATGAACA CGACGGCCGG GACAGTCTTG ATTTGGATCA CTCTTTCGAT
    GTCTTCAAAA AACCTAAGCA TCCCCCAGCG TTCTTTGCTG CAGGCGGGCT CTCTGATGTG
    CTGAATGGCG GCGATGAAGT TTACGCCAAC TGTATGGTGA TTGACCAGGT TGGTGATTTG
    GATATTAACT ACATCAATTT AGAAGGAATC TCTACAGACG CCTGTGCTGA ATCCATGGGT
    TCTACTCTGG GGCCTCAGAA CTGCGAGCAT ATCCTTGCTG CTGAAACCAG CCATGGGAAA
    TACCCACTAA GTGAGAACAA GGAAGTGGTC TCAGATACCG AAGCTCAGCA GTCCTTCCCA
    TCACCGTCTA GTTCATATGC TTCCAATTTT GATCTTTCCT TTGGTCTTCA TGGATTTGAG
    AAGGAACAAA GTCTCCTGAA GAAAAGAAGC TCCAGCCTGG ATGCCTTGGA TGCCGACAGC
    GAAGGGGAAG GGCATTCTGA GCGGTCACAC ATCTGCTACA CGCCAGCATC TCAGAGTTCC
    TCGAGGACTG GGATTCCCAG TGGGGATGAG CTGGACTCCT TCGAGACCGC CACCAAACCT
    GATTTTAGTA TCTCCAGGAC AGAGTCGCTT TCTTTATCAA GTAGCCTGCA GTTGAAGGAA
    TCATTGCTTC CTGGAGTCCG CTCACGTTCT TATTCCTGCT CATCACCCAA AATTTCTTTA
    GGAAAAACTC GATTGGTGCG TGATTTCACA GTGTGCAATT CTGGTGAAGA GCAAAGAGCC
    TTCGGCTTCC CGGAGCCCGC CAGGGAGAGG AGGATTCAAG AGGAAGAATG GGATAAATAC
    ATCATACCTG CCAAGTCAGA GTCTGAGAAA TACAAAGTGA GCCGGACCTT CAGCTTCCTC
    ATGAACAGGA TGACAAGCCC TCGGAACAAA TCTAAGACGA AAAGCAAGGA TGCCAAAGAC
    AAAGAGAAAC CGAGCCGACA TCAGTTTGTC CCCGGAACCT TCTCTGGGGT TCTCCAGTGT
    TCCGTTTGTG ATAAAACGCT CCTGGGGAAG GAGTCATCCC AGTGTTCCAA CTGTAATGCA
    AACGTCCACA AGGGTTGTAA GGACGCTGCA CCTCCGTGTA CCAAGAAATT CCAGGAGAAA
    TATAACAAGA ACAAACCGCA GACCATCCTT GGAAACTCTT CATTTCGAGA CATCCCGCAA
    CCCAGTCTCT CCTTGCACCC TTCTTCCTCC ATGCCTATCG GATTGCCCAC TGGGAGAAAA
    GAAGCTTCTG CCCAGGCCCA TCCCTTGTCC AGAAGTGTTC CAGGCACTGC CTTGGAAAGC
    TTCAGAAGAT CGGGGCTATC CCTGGAGTCT GAGAGTGACG GGAACAGCTG GAGAAGCAGG
    TCTCATTCCG ATGAGCTGTT ACAGTCCATG GGCTCTTCTC CCTCCACGGA ATCCTTCTTA
    ATGGAAGATG TCGTGGACTC TTCTCTATGG AGTGACCTCA GCAGTGACGC CCAGGAGTTG
    GAAGCAGAAT CCTGGAGTCT GGTGGTGGAC CCCTCCTTTT GCAATAAGCA GGAGAAAGAT
    GTCATCAAAA GACAGGATGT CATTTTCGAG CTTATGCAAA CAGAGATGCA TCATATCCAG
    ACCCTGCTCA TCATGTCTGA GATCTTCAGG AAAGGAATGA AGGAGGAGCT GCAGCTTGAT
    CACAGCACCG TGGATAAAAT CTTTCCCTGC TTAGATGAGT TACTTGAAAT CCACAGGCAT
    TTCTTTTATA GTATGAAGGA GCGAAGGCAG GAGTCCTGTG CTGGTGGTGA CAGGAATTTT
    GTCATCAGCC GAATTGGAGA TATTCTAGTA CAGCAGTTTT CAGAAGAAAA TGCAAATCAA
    ATGAAGAAAA TATATGGAGA ATTCTGTAGC CATCACAAAG AAGCTGTTAG CCTCTATAAA
    GAACTCCAAC AGAATAAGAA GTTTCAGAAT TTTATTAAGC TTCGAAATAG TAATCTTTTG
    GCTCGACGCC GAGGAATCCC AGAATGCATT CTGCTAGTCA CTCAGCGCAT CACAAAATAC
    CCTGTCCTGG TGGAACGGAT TTTGCAGTAC ACAAAGGAAA GAAGTGAAGA ACATCAAGAC
    TTGTGCAAAG CTCTGTGCTT AATTAAAGAC ATGATTGCAG CAGTGGATTT AAAAGTCAGC
    GAGTATGAGA AAAACCAAAA ATGGCTTGAG ATCCTAAATA AGATCGAAAA CAAAACCTAC
    ACAAAGCTCA AAAATGGCCA CGTGTTTAGG AAGCAAGCAC TGATAAGTAA AGAAAGGACT
    CTCTTATATG ATGGCCTTGT TTATTGGAAA ACTGCTACGG GTCGTTTCAA AGATATCCTG
    GCTCTACTTC TAACTGATGT GCTGCTCTTT TTACAAGAAA AAGACCAGAA ATACATCTTT
    GCAGCTGTTG ATCAGAAGCC ATCGGTTATT TCCCTTCAGA AGCTCATTGC TAGAGAAGTC
    GCCAACGAGG AAAGAGGAAT GTTCCTGATC AGTGCATCCT CTGCTGGTCC TGAGATGTAC
    GAAATTCACA CCAACTCCAA GGAGGAACGA AATAACTGGA TGAGACGAAT CCAACAGGCC
    GTGGAAAGTT GCCCAGAAGA AGAAGGGGGA AGGACAAGTG AATCTGATGA AGAGAGGCGG
    AAAGCTGAAG CAAGAGTGGC CAAAATCCAG CAATGTCAAG AAATACTCAG TAACCAAGAC
    CAACAGATTT GCGCATATTT GGAGGAGAAG CTGCATATCT ACGCTGAACT TGGAGAACTG
    AGTGGATTCA AGGATGTCCA TCTAGAGCCC CACCTCCTCA TCAAACCTGA CCCAGGGGAG
    CCTCCCCAGG CAGCCTCATT ACTAGCAGCA GCCCTGAGAG AAGCGGAGAG CCTGCAGGTT
    GCCGTGAAGG CCTCACAAGC GGGTGATGCT CGCCCATCAC CTGAGGAGGG TTCTGCAGAA
    CCTGCGGTTG CAGATGCACA GGGTCCTCCC GAGGAGCCAG GATCATCTCC AGCCTCACTA
    GTCACAGAAG GGACAGGAGG AAGAGGCTGT TGGGATGTGG ATCCTGGCCC CCAGGGTGTG
    GGAACCGACT TGGCGGTCTC TGATGCAGGG GAGAAGAGTC TTAAAAATCT GTGCTGCAGC
    CGGCAAAGTC CCTGGGGTGT CAGTCACCAT CACCAGCTGC AGCCCTGCAC AGGGACCACA
    AGGGGGAGGG CCAGCTCCAC GAAAAGTAGG ACACTACACA GCTCAGGCTT GTATCAGGCT
    TCGTGGTTCT TGGGAGTGGA ATATAGAAGT TTCACGGTTT CTTCACAGTC AGAGATTATA
    CAAGCGATCC AGAATTTAAC CCGACTCCTC TATAGCCTTC AGGTGATGGA ACTTAATCGA
    TCCGAGAGCT TGTGTCATGA AAATTCATTC TTCATTAATG AAGCTTTAGT ACAAATGTCA
    CTTAACACTT ACAACAAATC AACTCCATCA GGTGTCCACC AGGAAGCCGC TTATCCCCAG
    AATATAGCTA ATTCGGACTT GGTAAGGACT AGTGAAAGTC CCGTAGACCT CCAGTTGGAC
    ATTTCTCAGC CCTTGGATGT CAACCACGAA CTGTGGACAG CCGCTGGGTC CTGTCATCAG
    ATACCCCCTC TCCACGAAAG CAGCAAGGAT TCTTGTAAAA ATGGAAGAAT TTACATCAGT
    CAGCTATGTT TCCAGAGCCA TTCGTCAGAA ATGTTTTGGC GACCAGACTT GACCATCTCA
    GCTTGGGATA GAGAGCTTCC GAATCTGCAG AACTTCCAAC CGCTGTTGGT TGCATTATGT
    AGATGGTTGC GTTACGTCCG TACATCCCCG GGAGATCTTG CCGTCATCCT CGAGTCCCCT
    GAGCAGGTTT TTCAGCTAGA AAGAAAATGT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03220
    Clone ID Related Accession (Same CDS sequence) XM_005617471.2 , XM_005617471.3
    Accession Version XM_005617471.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5148bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product rho guanine nucleotide exchange factor 28 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005617471.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGTTGA GCTGCAGCGA AGTACCTCTT TATGGGCAGA TGACCGTCTA TGCAAAGTTT 
    GGGAAAAATG TTTATCTTCC TAAAGATGCC GAGTTTTACT TTATTTATAA TGGATCTCAT
    CAGAGACACG TCGTGATTGC AAAACGCACT GAGGATAATG TTCTCCAGTC CAGCATTCCA
    GGGCATGGGC TTCAGGAGAC GGTGACGGTG TCTGTATGCC TCTGCTCAGA AGGTTACTCC
    CCAGTGACCA TGGGCTCCGG CTCAGTGACC TACGTGGACA ACATGGCTTG CAGGCTGGCT
    CGTTTGCTGG TCACCCAGGC TGATCGCCTC ACAGCCAGTA GTCATCAGAC CTTTCTGACT
    CCATTTGCCC TGACGGTGGG AGCATTGCCT GCTTTGGACG AGGAGCTGGT GCTGGCTCTG
    ACCCATCTGG AGTTGCCTCT TGAGTGGAGT GTCTTGGGAA ATTCTTCACT TGAAGTTTCT
    TTATGCAGAG AGTCTCTTCT ACACCTGGCT ATGAGATGGG GCCTGCCTAA GCTCTCTCAT
    TTCCTCTTGT GCCTCCCTGG GGGAGTTCAG GCTTTGTCTT TACCCAATGA AGAAGGTGCC
    ACACCATTAG ACTTGGCTTT ACGTGCCGGA CACTCCAAGC TGGTTGAAGA CATCACAAAT
    TTCCAGGGCA GACGTTCCCC CGACCTCTCC CGAGTGCACC TCGGCGGACG TGCCTTCCTG
    CAGTATGTTC ACCCGTCGGG GACACTGACG CTGACGCTGA ACCACACAGC TGAGCATTTG
    TTGGAGGCAG ATATTAAACT CTTCAGGAAA TACTTTTTGG ACAGAGCCTT TCTCCTCAAG
    GCTCTTGACG ACCGAGAAGC TGGGCCAGAG GAGAGCCGGG GCATGCCCTC CCGGGCTGCC
    GCCACTGAGG AGGAGATTAG GAATTTGGTG ACCGGCAGGC CATCACCTGA AAAGGAGGAT
    GTAAAGCGTG TGGACAGCCC GGACGTTGAC CCCCATGAAC ACGACGGCCG GGACAGTCTT
    GATTTGGATC ACTCTTTCGA TGTCTTCAAA AAACCTAAGC ATCCCCCAGC GTTCTTTGCT
    GCAGGCGGGC TCTCTGATGT GCTGAATGGC GGCGATGAAG TTTACGCCAA CTGTATGGTG
    ATTGACCAGG TTGGTGATTT GGATATTAAC TACATCAATT TAGAAGGAAT CTCTACAGAC
    GCCTGTGCTG AATCCATGGG TTCTACTCTG GGGCCTCAGA ACTGCGAGCA TATCCTTGCT
    GCTGAAACCA GCCATGGGAA ATACCCACTA AGTGAGAACA AGGAAGTGGT CTCAGATACC
    GAAGCTCAGC AGTCCTTCCC ATCACCGTCT AGTTCATATG CTTCCAATTT TGATCTTTCC
    TTTGGTCTTC ATGGATTTGA GAAGGAACAA AGTCTCCTGA AGAAAAGAAG CTCCAGCCTG
    GATGCCTTGG ATGCCGACAG CGAAGGGGAA GGGCATTCTG AGCGGTCACA CATCTGCTAC
    ACGCCAGCAT CTCAGAGTTC CTCGAGGACT GGGATTCCCA GTGGGGATGA GCTGGACTCC
    TTCGAGACCG CCACCAAACC TGATTTTAGT ATCTCCAGGA CAGAGTCGCT TTCTTTATCA
    AGTAGCCTGC AGTTGAAGGA ATCATTGCTT CCTGGAGTCC GCTCACGTTC TTATTCCTGC
    TCATCACCCA AAATTTCTTT AGGAAAAACT CGATTGGTGC GTGATTTCAC AGTGTGCAAT
    TCTGGTGAAG AGCAAAGAGC CTTCGGCTTC CCGGAGCCCG CCAGGGAGAG GAGGATTCAA
    GAGGAAGAAT GGGATAAATA CATCATACCT GCCAAGTCAG AGTCTGAGAA ATACAAAGTG
    AGCCGGACCT TCAGCTTCCT CATGAACAGG ATGACAAGCC CTCGGAACAA ATCTAAGACG
    AAAAGCAAGG ATGCCAAAGA CAAAGAGAAA CCGAGCCGAC ATCAGTTTGT CCCCGGAACC
    TTCTCTGGGG TTCTCCAGTG TTCCGTTTGT GATAAAACGC TCCTGGGGAA GGAGTCATCC
    CAGTGTTCCA ACTGTAATGC AAACGTCCAC AAGGGTTGTA AGGACGCTGC ACCTCCGTGT
    ACCAAGAAAT TCCAGGAGAA ATATAACAAG AACAAACCGC AGACCATCCT TGGAAACTCT
    TCATTTCGAG ACATCCCGCA ACCCAGTCTC TCCTTGCACC CTTCTTCCTC CATGCCTATC
    GGATTGCCCA CTGGGAGAAA AGAAGCTTCT GCCCAGGCCC ATCCCTTGTC CAGAAGTGTT
    CCAGGCACTG CCTTGGAAAG CTTCAGAAGA TCGGGGCTAT CCCTGGAGTC TGAGAGTGAC
    GGGAACAGCT GGAGAAGCAG GTCTCATTCC GATGAGCTGT TACAGTCCAT GGGCTCTTCT
    CCCTCCACGG AATCCTTCTT AATGGAAGAT GTCGTGGACT CTTCTCTATG GAGTGACCTC
    AGCAGTGACG CCCAGGAGTT GGAAGCAGAA TCCTGGAGTC TGGTGGTGGA CCCCTCCTTT
    TGCAATAAGC AGGAGAAAGA TGTCATCAAA AGACAGGATG TCATTTTCGA GCTTATGCAA
    ACAGAGATGC ATCATATCCA GACCCTGCTC ATCATGTCTG AGATCTTCAG GAAAGGAATG
    AAGGAGGAGC TGCAGCTTGA TCACAGCACC GTGGATAAAA TCTTTCCCTG CTTAGATGAG
    TTACTTGAAA TCCACAGGCA TTTCTTTTAT AGTATGAAGG AGCGAAGGCA GGAGTCCTGT
    GCTGGTGGTG ACAGGAATTT TGTCATCAGC CGAATTGGAG ATATTCTAGT ACAGCAGTTT
    TCAGAAGAAA ATGCAAATCA AATGAAGAAA ATATATGGAG AATTCTGTAG CCATCACAAA
    GAAGCTGTTA GCCTCTATAA AGAACTCCAA CAGAATAAGA AGTTTCAGAA TTTTATTAAG
    CTTCGAAATA GTAATCTTTT GGCTCGACGC CGAGGAATCC CAGAATGCAT TCTGCTAGTC
    ACTCAGCGCA TCACAAAATA CCCTGTCCTG GTGGAACGGA TTTTGCAGTA CACAAAGGAA
    AGAAGTGAAG AACATCAAGA CTTGTGCAAA GCTCTGTGCT TAATTAAAGA CATGATTGCA
    GCAGTGGATT TAAAAGTCAG CGAGTATGAG AAAAACCAAA AATGGCTTGA GATCCTAAAT
    AAGATCGAAA ACAAAACCTA CACAAAGCTC AAAAATGGCC ACGTGTTTAG GAAGCAAGCA
    CTGATAAGTA AAGAAAGGAC TCTCTTATAT GATGGCCTTG TTTATTGGAA AACTGCTACG
    GGTCGTTTCA AAGATATCCT GGCTCTACTT CTAACTGATG TGCTGCTCTT TTTACAAGAA
    AAAGACCAGA AATACATCTT TGCAGCTGTT GATCAGAAGC CATCGGTTAT TTCCCTTCAG
    AAGCTCATTG CTAGAGAAGT CGCCAACGAG GAAAGAGGAA TGTTCCTGAT CAGTGCATCC
    TCTGCTGGTC CTGAGATGTA CGAAATTCAC ACCAACTCCA AGGAGGAACG AAATAACTGG
    ATGAGACGAA TCCAACAGGC CGTGGAAAGT TGCCCAGAAG AAGAAGGGGG AAGGACAAGT
    GAATCTGATG AAGAGAGGCG GAAAGCTGAA GCAAGAGTGG CCAAAATCCA GCAATGTCAA
    GAAATACTCA GTAACCAAGA CCAACAGATT TGCGCATATT TGGAGGAGAA GCTGCATATC
    TACGCTGAAC TTGGAGAACT GAGTGGATTC AAGGATGTCC ATCTAGAGCC CCACCTCCTC
    ATCAAACCTG ACCCAGGGGA GCCTCCCCAG GCAGCCTCAT TACTAGCAGC AGCCCTGAGA
    GAAGCGGAGA GCCTGCAGGT TGCCGTGAAG GCCTCACAAG CGGGTGATGC TCGCCCATCA
    CCTGAGGAGG GTTCTGCAGA ACCTGCGGTT GCAGATGCAC AGGGTCCTCC CGAGGAGCCA
    GGATCATCTC CAGCCTCACT AGTCACAGAA GGGACAGGAG GAAGAGGCTG TTGGGATGTG
    GATCCTGGCC CCCAGGGTGT GGGAACCGAC TTGGCGGTCT CTGATGCAGG GGAGAAGGTG
    GAATATAGAA GTTTCACGGT TTCTTCACAG TCAGAGATTA TACAAGCGAT CCAGAATTTA
    ACCCGACTCC TCTATAGCCT TCAGGCCGCG CTGACCATCC AGGACAGCCA CATCCAGGTG
    CACAGGCTGC TGCTGCAGCA GGCTGGCCTG CCGGCGTCCC AAGAGGAGCA CGGCGAGCTG
    GCGAGGCAGC GGGAGGAGCT GGCCGCCCTG CCCGGGCTCC AGTGGCAGCT GCAGCAGGAG
    CGGCGGCGCG GCCAGCGCAC CTGGGAGCGG CAGCAGCGCG AGCAGGAGGC CAGGGCCTGC
    CGGCTGCGGG AGCGCGAGCA GGGCTGCCGC GGCCGCGAGG AGCTGCTGCT CAGGGCCCGC
    GGGGAGCTGG ACCGGCAGCT GCGGGAGCAC CGGCAGGACC TGGAGCGGCT GCGGGAGGGC
    CAGCGCCTGG TGGAGAGTGC GAGGGCCCGC ACGCGCGCCC AGCACAGCCT GCTGCGTGGC
    CCGAGGCCCG GGCGCCAGAG CAGCCTGCCC GCGGCCTTGC CTCCGGGCAG CGGCGAGGTG
    ATGGAACTTA ATCGATCCGA GAGCTTGTGT CATGAAAATT CATTCTTCAT TAATGAAGCT
    TTAGTACAAA TGTCACTTAA CACTTACAAC AAATCAACTC CATCAGGTGT CCACCAGGAA
    GCCGCTTATC CCCAGAATAT AGCTAATTCG GACTTGGTAA GGACTAGTGA AAGTCCCGTA
    GACCTCCAGT TGGACATTTC TCAGCCCTTG GATGTCAACC ACGAACTGTG GACAGCCGCT
    GGGTCCTGTC ATCAGATACC CCCTCTCCAC GAAAGCAGCA AGGATTCTTG TAAAAATGGA
    AGAATTTACA TCAGTCAGCT ATGTTTCCAG AGCCATTCGT CAGAAATGTT TTGGCGACCA
    GACTTGACCA TCTCAGCTTG GGATAGAGAG CTTCCGAATC TGCAGAACTT CCAACCGCTG
    TTGGTTGCAT TATGTAGATG GTTGCGTTAC GTCCGTACAT CCCCGGGAGA TCTTGCCGTC
    ATCCTCGAGT CCCCTGAGCA GGTTTTTCAG CTAGAAAGAA AATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005617528.1
    CDS12..5159
    Translation

    Target ORF information:

    RefSeq Version XM_005617471.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 28 (ARHGEF28), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005617471.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGTTGA GCTGCAGCGA AGTACCTCTT TATGGGCAGA TGACCGTCTA TGCAAAGTTT 
    GGGAAAAATG TTTATCTTCC TAAAGATGCC GAGTTTTACT TTATTTATAA TGGATCTCAT
    CAGAGACACG TCGTGATTGC AAAACGCACT GAGGATAATG TTCTCCAGTC CAGCATTCCA
    GGGCATGGGC TTCAGGAGAC GGTGACGGTG TCTGTATGCC TCTGCTCAGA AGGTTACTCC
    CCAGTGACCA TGGGCTCCGG CTCAGTGACC TACGTGGACA ACATGGCTTG CAGGCTGGCT
    CGTTTGCTGG TCACCCAGGC TGATCGCCTC ACAGCCAGTA GTCATCAGAC CTTTCTGACT
    CCATTTGCCC TGACGGTGGG AGCATTGCCT GCTTTGGACG AGGAGCTGGT GCTGGCTCTG
    ACCCATCTGG AGTTGCCTCT TGAGTGGAGT GTCTTGGGAA ATTCTTCACT TGAAGTTTCT
    TTATGCAGAG AGTCTCTTCT ACACCTGGCT ATGAGATGGG GCCTGCCTAA GCTCTCTCAT
    TTCCTCTTGT GCCTCCCTGG GGGAGTTCAG GCTTTGTCTT TACCCAATGA AGAAGGTGCC
    ACACCATTAG ACTTGGCTTT ACGTGCCGGA CACTCCAAGC TGGTTGAAGA CATCACAAAT
    TTCCAGGGCA GACGTTCCCC CGACCTCTCC CGAGTGCACC TCGGCGGACG TGCCTTCCTG
    CAGTATGTTC ACCCGTCGGG GACACTGACG CTGACGCTGA ACCACACAGC TGAGCATTTG
    TTGGAGGCAG ATATTAAACT CTTCAGGAAA TACTTTTTGG ACAGAGCCTT TCTCCTCAAG
    GCTCTTGACG ACCGAGAAGC TGGGCCAGAG GAGAGCCGGG GCATGCCCTC CCGGGCTGCC
    GCCACTGAGG AGGAGATTAG GAATTTGGTG ACCGGCAGGC CATCACCTGA AAAGGAGGAT
    GTAAAGCGTG TGGACAGCCC GGACGTTGAC CCCCATGAAC ACGACGGCCG GGACAGTCTT
    GATTTGGATC ACTCTTTCGA TGTCTTCAAA AAACCTAAGC ATCCCCCAGC GTTCTTTGCT
    GCAGGCGGGC TCTCTGATGT GCTGAATGGC GGCGATGAAG TTTACGCCAA CTGTATGGTG
    ATTGACCAGG TTGGTGATTT GGATATTAAC TACATCAATT TAGAAGGAAT CTCTACAGAC
    GCCTGTGCTG AATCCATGGG TTCTACTCTG GGGCCTCAGA ACTGCGAGCA TATCCTTGCT
    GCTGAAACCA GCCATGGGAA ATACCCACTA AGTGAGAACA AGGAAGTGGT CTCAGATACC
    GAAGCTCAGC AGTCCTTCCC ATCACCGTCT AGTTCATATG CTTCCAATTT TGATCTTTCC
    TTTGGTCTTC ATGGATTTGA GAAGGAACAA AGTCTCCTGA AGAAAAGAAG CTCCAGCCTG
    GATGCCTTGG ATGCCGACAG CGAAGGGGAA GGGCATTCTG AGCGGTCACA CATCTGCTAC
    ACGCCAGCAT CTCAGAGTTC CTCGAGGACT GGGATTCCCA GTGGGGATGA GCTGGACTCC
    TTCGAGACCG CCACCAAACC TGATTTTAGT ATCTCCAGGA CAGAGTCGCT TTCTTTATCA
    AGTAGCCTGC AGTTGAAGGA ATCATTGCTT CCTGGAGTCC GCTCACGTTC TTATTCCTGC
    TCATCACCCA AAATTTCTTT AGGAAAAACT CGATTGGTGC GTGATTTCAC AGTGTGCAAT
    TCTGGTGAAG AGCAAAGAGC CTTCGGCTTC CCGGAGCCCG CCAGGGAGAG GAGGATTCAA
    GAGGAAGAAT GGGATAAATA CATCATACCT GCCAAGTCAG AGTCTGAGAA ATACAAAGTG
    AGCCGGACCT TCAGCTTCCT CATGAACAGG ATGACAAGCC CTCGGAACAA ATCTAAGACG
    AAAAGCAAGG ATGCCAAAGA CAAAGAGAAA CCGAGCCGAC ATCAGTTTGT CCCCGGAACC
    TTCTCTGGGG TTCTCCAGTG TTCCGTTTGT GATAAAACGC TCCTGGGGAA GGAGTCATCC
    CAGTGTTCCA ACTGTAATGC AAACGTCCAC AAGGGTTGTA AGGACGCTGC ACCTCCGTGT
    ACCAAGAAAT TCCAGGAGAA ATATAACAAG AACAAACCGC AGACCATCCT TGGAAACTCT
    TCATTTCGAG ACATCCCGCA ACCCAGTCTC TCCTTGCACC CTTCTTCCTC CATGCCTATC
    GGATTGCCCA CTGGGAGAAA AGAAGCTTCT GCCCAGGCCC ATCCCTTGTC CAGAAGTGTT
    CCAGGCACTG CCTTGGAAAG CTTCAGAAGA TCGGGGCTAT CCCTGGAGTC TGAGAGTGAC
    GGGAACAGCT GGAGAAGCAG GTCTCATTCC GATGAGCTGT TACAGTCCAT GGGCTCTTCT
    CCCTCCACGG AATCCTTCTT AATGGAAGAT GTCGTGGACT CTTCTCTATG GAGTGACCTC
    AGCAGTGACG CCCAGGAGTT GGAAGCAGAA TCCTGGAGTC TGGTGGTGGA CCCCTCCTTT
    TGCAATAAGC AGGAGAAAGA TGTCATCAAA AGACAGGATG TCATTTTCGA GCTTATGCAA
    ACAGAGATGC ATCATATCCA GACCCTGCTC ATCATGTCTG AGATCTTCAG GAAAGGAATG
    AAGGAGGAGC TGCAGCTTGA TCACAGCACC GTGGATAAAA TCTTTCCCTG CTTAGATGAG
    TTACTTGAAA TCCACAGGCA TTTCTTTTAT AGTATGAAGG AGCGAAGGCA GGAGTCCTGT
    GCTGGTGGTG ACAGGAATTT TGTCATCAGC CGAATTGGAG ATATTCTAGT ACAGCAGTTT
    TCAGAAGAAA ATGCAAATCA AATGAAGAAA ATATATGGAG AATTCTGTAG CCATCACAAA
    GAAGCTGTTA GCCTCTATAA AGAACTCCAA CAGAATAAGA AGTTTCAGAA TTTTATTAAG
    CTTCGAAATA GTAATCTTTT GGCTCGACGC CGAGGAATCC CAGAATGCAT TCTGCTAGTC
    ACTCAGCGCA TCACAAAATA CCCTGTCCTG GTGGAACGGA TTTTGCAGTA CACAAAGGAA
    AGAAGTGAAG AACATCAAGA CTTGTGCAAA GCTCTGTGCT TAATTAAAGA CATGATTGCA
    GCAGTGGATT TAAAAGTCAG CGAGTATGAG AAAAACCAAA AATGGCTTGA GATCCTAAAT
    AAGATCGAAA ACAAAACCTA CACAAAGCTC AAAAATGGCC ACGTGTTTAG GAAGCAAGCA
    CTGATAAGTA AAGAAAGGAC TCTCTTATAT GATGGCCTTG TTTATTGGAA AACTGCTACG
    GGTCGTTTCA AAGATATCCT GGCTCTACTT CTAACTGATG TGCTGCTCTT TTTACAAGAA
    AAAGACCAGA AATACATCTT TGCAGCTGTT GATCAGAAGC CATCGGTTAT TTCCCTTCAG
    AAGCTCATTG CTAGAGAAGT CGCCAACGAG GAAAGAGGAA TGTTCCTGAT CAGTGCATCC
    TCTGCTGGTC CTGAGATGTA CGAAATTCAC ACCAACTCCA AGGAGGAACG AAATAACTGG
    ATGAGACGAA TCCAACAGGC CGTGGAAAGT TGCCCAGAAG AAGAAGGGGG AAGGACAAGT
    GAATCTGATG AAGAGAGGCG GAAAGCTGAA GCAAGAGTGG CCAAAATCCA GCAATGTCAA
    GAAATACTCA GTAACCAAGA CCAACAGATT TGCGCATATT TGGAGGAGAA GCTGCATATC
    TACGCTGAAC TTGGAGAACT GAGTGGATTC AAGGATGTCC ATCTAGAGCC CCACCTCCTC
    ATCAAACCTG ACCCAGGGGA GCCTCCCCAG GCAGCCTCAT TACTAGCAGC AGCCCTGAGA
    GAAGCGGAGA GCCTGCAGGT TGCCGTGAAG GCCTCACAAG CGGGTGATGC TCGCCCATCA
    CCTGAGGAGG GTTCTGCAGA ACCTGCGGTT GCAGATGCAC AGGGTCCTCC CGAGGAGCCA
    GGATCATCTC CAGCCTCACT AGTCACAGAA GGGACAGGAG GAAGAGGCTG TTGGGATGTG
    GATCCTGGCC CCCAGGGTGT GGGAACCGAC TTGGCGGTCT CTGATGCAGG GGAGAAGGTG
    GAATATAGAA GTTTCACGGT TTCTTCACAG TCAGAGATTA TACAAGCGAT CCAGAATTTA
    ACCCGACTCC TCTATAGCCT TCAGGCCGCG CTGACCATCC AGGACAGCCA CATCCAGGTG
    CACAGGCTGC TGCTGCAGCA GGCTGGCCTG CCGGCGTCCC AAGAGGAGCA CGGCGAGCTG
    GCGAGGCAGC GGGAGGAGCT GGCCGCCCTG CCCGGGCTCC AGTGGCAGCT GCAGCAGGAG
    CGGCGGCGCG GCCAGCGCAC CTGGGAGCGG CAGCAGCGCG AGCAGGAGGC CAGGGCCTGC
    CGGCTGCGGG AGCGCGAGCA GGGCTGCCGC GGCCGCGAGG AGCTGCTGCT CAGGGCCCGC
    GGGGAGCTGG ACCGGCAGCT GCGGGAGCAC CGGCAGGACC TGGAGCGGCT GCGGGAGGGC
    CAGCGCCTGG TGGAGAGTGC GAGGGCCCGC ACGCGCGCCC AGCACAGCCT GCTGCGTGGC
    CCGAGGCCCG GGCGCCAGAG CAGCCTGCCC GCGGCCTTGC CTCCGGGCAG CGGCGAGGTG
    ATGGAACTTA ATCGATCCGA GAGCTTGTGT CATGAAAATT CATTCTTCAT TAATGAAGCT
    TTAGTACAAA TGTCACTTAA CACTTACAAC AAATCAACTC CATCAGGTGT CCACCAGGAA
    GCCGCTTATC CCCAGAATAT AGCTAATTCG GACTTGGTAA GGACTAGTGA AAGTCCCGTA
    GACCTCCAGT TGGACATTTC TCAGCCCTTG GATGTCAACC ACGAACTGTG GACAGCCGCT
    GGGTCCTGTC ATCAGATACC CCCTCTCCAC GAAAGCAGCA AGGATTCTTG TAAAAATGGA
    AGAATTTACA TCAGTCAGCT ATGTTTCCAG AGCCATTCGT CAGAAATGTT TTGGCGACCA
    GACTTGACCA TCTCAGCTTG GGATAGAGAG CTTCCGAATC TGCAGAACTT CCAACCGCTG
    TTGGTTGCAT TATGTAGATG GTTGCGTTAC GTCCGTACAT CCCCGGGAGA TCTTGCCGTC
    ATCCTCGAGT CCCCTGAGCA GGTTTTTCAG CTAGAAAGAA AATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03220
    Clone ID Related Accession (Same CDS sequence) XM_005617471.2 , XM_005617471.3
    Accession Version XM_005617471.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5148bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rho guanine nucleotide exchange factor 28 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005617471.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGTTGA GCTGCAGCGA AGTACCTCTT TATGGGCAGA TGACCGTCTA TGCAAAGTTT 
    GGGAAAAATG TTTATCTTCC TAAAGATGCC GAGTTTTACT TTATTTATAA TGGATCTCAT
    CAGAGACACG TCGTGATTGC AAAACGCACT GAGGATAATG TTCTCCAGTC CAGCATTCCA
    GGGCATGGGC TTCAGGAGAC GGTGACGGTG TCTGTATGCC TCTGCTCAGA AGGTTACTCC
    CCAGTGACCA TGGGCTCCGG CTCAGTGACC TACGTGGACA ACATGGCTTG CAGGCTGGCT
    CGTTTGCTGG TCACCCAGGC TGATCGCCTC ACAGCCAGTA GTCATCAGAC CTTTCTGACT
    CCATTTGCCC TGACGGTGGG AGCATTGCCT GCTTTGGACG AGGAGCTGGT GCTGGCTCTG
    ACCCATCTGG AGTTGCCTCT TGAGTGGAGT GTCTTGGGAA ATTCTTCACT TGAAGTTTCT
    TTATGCAGAG AGTCTCTTCT ACACCTGGCT ATGAGATGGG GCCTGCCTAA GCTCTCTCAT
    TTCCTCTTGT GCCTCCCTGG GGGAGTTCAG GCTTTGTCTT TACCCAATGA AGAAGGTGCC
    ACACCATTAG ACTTGGCTTT ACGTGCCGGA CACTCCAAGC TGGTTGAAGA CATCACAAAT
    TTCCAGGGCA GACGTTCCCC CGACCTCTCC CGAGTGCACC TCGGCGGACG TGCCTTCCTG
    CAGTATGTTC ACCCGTCGGG GACACTGACG CTGACGCTGA ACCACACAGC TGAGCATTTG
    TTGGAGGCAG ATATTAAACT CTTCAGGAAA TACTTTTTGG ACAGAGCCTT TCTCCTCAAG
    GCTCTTGACG ACCGAGAAGC TGGGCCAGAG GAGAGCCGGG GCATGCCCTC CCGGGCTGCC
    GCCACTGAGG AGGAGATTAG GAATTTGGTG ACCGGCAGGC CATCACCTGA AAAGGAGGAT
    GTAAAGCGTG TGGACAGCCC GGACGTTGAC CCCCATGAAC ACGACGGCCG GGACAGTCTT
    GATTTGGATC ACTCTTTCGA TGTCTTCAAA AAACCTAAGC ATCCCCCAGC GTTCTTTGCT
    GCAGGCGGGC TCTCTGATGT GCTGAATGGC GGCGATGAAG TTTACGCCAA CTGTATGGTG
    ATTGACCAGG TTGGTGATTT GGATATTAAC TACATCAATT TAGAAGGAAT CTCTACAGAC
    GCCTGTGCTG AATCCATGGG TTCTACTCTG GGGCCTCAGA ACTGCGAGCA TATCCTTGCT
    GCTGAAACCA GCCATGGGAA ATACCCACTA AGTGAGAACA AGGAAGTGGT CTCAGATACC
    GAAGCTCAGC AGTCCTTCCC ATCACCGTCT AGTTCATATG CTTCCAATTT TGATCTTTCC
    TTTGGTCTTC ATGGATTTGA GAAGGAACAA AGTCTCCTGA AGAAAAGAAG CTCCAGCCTG
    GATGCCTTGG ATGCCGACAG CGAAGGGGAA GGGCATTCTG AGCGGTCACA CATCTGCTAC
    ACGCCAGCAT CTCAGAGTTC CTCGAGGACT GGGATTCCCA GTGGGGATGA GCTGGACTCC
    TTCGAGACCG CCACCAAACC TGATTTTAGT ATCTCCAGGA CAGAGTCGCT TTCTTTATCA
    AGTAGCCTGC AGTTGAAGGA ATCATTGCTT CCTGGAGTCC GCTCACGTTC TTATTCCTGC
    TCATCACCCA AAATTTCTTT AGGAAAAACT CGATTGGTGC GTGATTTCAC AGTGTGCAAT
    TCTGGTGAAG AGCAAAGAGC CTTCGGCTTC CCGGAGCCCG CCAGGGAGAG GAGGATTCAA
    GAGGAAGAAT GGGATAAATA CATCATACCT GCCAAGTCAG AGTCTGAGAA ATACAAAGTG
    AGCCGGACCT TCAGCTTCCT CATGAACAGG ATGACAAGCC CTCGGAACAA ATCTAAGACG
    AAAAGCAAGG ATGCCAAAGA CAAAGAGAAA CCGAGCCGAC ATCAGTTTGT CCCCGGAACC
    TTCTCTGGGG TTCTCCAGTG TTCCGTTTGT GATAAAACGC TCCTGGGGAA GGAGTCATCC
    CAGTGTTCCA ACTGTAATGC AAACGTCCAC AAGGGTTGTA AGGACGCTGC ACCTCCGTGT
    ACCAAGAAAT TCCAGGAGAA ATATAACAAG AACAAACCGC AGACCATCCT TGGAAACTCT
    TCATTTCGAG ACATCCCGCA ACCCAGTCTC TCCTTGCACC CTTCTTCCTC CATGCCTATC
    GGATTGCCCA CTGGGAGAAA AGAAGCTTCT GCCCAGGCCC ATCCCTTGTC CAGAAGTGTT
    CCAGGCACTG CCTTGGAAAG CTTCAGAAGA TCGGGGCTAT CCCTGGAGTC TGAGAGTGAC
    GGGAACAGCT GGAGAAGCAG GTCTCATTCC GATGAGCTGT TACAGTCCAT GGGCTCTTCT
    CCCTCCACGG AATCCTTCTT AATGGAAGAT GTCGTGGACT CTTCTCTATG GAGTGACCTC
    AGCAGTGACG CCCAGGAGTT GGAAGCAGAA TCCTGGAGTC TGGTGGTGGA CCCCTCCTTT
    TGCAATAAGC AGGAGAAAGA TGTCATCAAA AGACAGGATG TCATTTTCGA GCTTATGCAA
    ACAGAGATGC ATCATATCCA GACCCTGCTC ATCATGTCTG AGATCTTCAG GAAAGGAATG
    AAGGAGGAGC TGCAGCTTGA TCACAGCACC GTGGATAAAA TCTTTCCCTG CTTAGATGAG
    TTACTTGAAA TCCACAGGCA TTTCTTTTAT AGTATGAAGG AGCGAAGGCA GGAGTCCTGT
    GCTGGTGGTG ACAGGAATTT TGTCATCAGC CGAATTGGAG ATATTCTAGT ACAGCAGTTT
    TCAGAAGAAA ATGCAAATCA AATGAAGAAA ATATATGGAG AATTCTGTAG CCATCACAAA
    GAAGCTGTTA GCCTCTATAA AGAACTCCAA CAGAATAAGA AGTTTCAGAA TTTTATTAAG
    CTTCGAAATA GTAATCTTTT GGCTCGACGC CGAGGAATCC CAGAATGCAT TCTGCTAGTC
    ACTCAGCGCA TCACAAAATA CCCTGTCCTG GTGGAACGGA TTTTGCAGTA CACAAAGGAA
    AGAAGTGAAG AACATCAAGA CTTGTGCAAA GCTCTGTGCT TAATTAAAGA CATGATTGCA
    GCAGTGGATT TAAAAGTCAG CGAGTATGAG AAAAACCAAA AATGGCTTGA GATCCTAAAT
    AAGATCGAAA ACAAAACCTA CACAAAGCTC AAAAATGGCC ACGTGTTTAG GAAGCAAGCA
    CTGATAAGTA AAGAAAGGAC TCTCTTATAT GATGGCCTTG TTTATTGGAA AACTGCTACG
    GGTCGTTTCA AAGATATCCT GGCTCTACTT CTAACTGATG TGCTGCTCTT TTTACAAGAA
    AAAGACCAGA AATACATCTT TGCAGCTGTT GATCAGAAGC CATCGGTTAT TTCCCTTCAG
    AAGCTCATTG CTAGAGAAGT CGCCAACGAG GAAAGAGGAA TGTTCCTGAT CAGTGCATCC
    TCTGCTGGTC CTGAGATGTA CGAAATTCAC ACCAACTCCA AGGAGGAACG AAATAACTGG
    ATGAGACGAA TCCAACAGGC CGTGGAAAGT TGCCCAGAAG AAGAAGGGGG AAGGACAAGT
    GAATCTGATG AAGAGAGGCG GAAAGCTGAA GCAAGAGTGG CCAAAATCCA GCAATGTCAA
    GAAATACTCA GTAACCAAGA CCAACAGATT TGCGCATATT TGGAGGAGAA GCTGCATATC
    TACGCTGAAC TTGGAGAACT GAGTGGATTC AAGGATGTCC ATCTAGAGCC CCACCTCCTC
    ATCAAACCTG ACCCAGGGGA GCCTCCCCAG GCAGCCTCAT TACTAGCAGC AGCCCTGAGA
    GAAGCGGAGA GCCTGCAGGT TGCCGTGAAG GCCTCACAAG CGGGTGATGC TCGCCCATCA
    CCTGAGGAGG GTTCTGCAGA ACCTGCGGTT GCAGATGCAC AGGGTCCTCC CGAGGAGCCA
    GGATCATCTC CAGCCTCACT AGTCACAGAA GGGACAGGAG GAAGAGGCTG TTGGGATGTG
    GATCCTGGCC CCCAGGGTGT GGGAACCGAC TTGGCGGTCT CTGATGCAGG GGAGAAGGTG
    GAATATAGAA GTTTCACGGT TTCTTCACAG TCAGAGATTA TACAAGCGAT CCAGAATTTA
    ACCCGACTCC TCTATAGCCT TCAGGCCGCG CTGACCATCC AGGACAGCCA CATCCAGGTG
    CACAGGCTGC TGCTGCAGCA GGCTGGCCTG CCGGCGTCCC AAGAGGAGCA CGGCGAGCTG
    GCGAGGCAGC GGGAGGAGCT GGCCGCCCTG CCCGGGCTCC AGTGGCAGCT GCAGCAGGAG
    CGGCGGCGCG GCCAGCGCAC CTGGGAGCGG CAGCAGCGCG AGCAGGAGGC CAGGGCCTGC
    CGGCTGCGGG AGCGCGAGCA GGGCTGCCGC GGCCGCGAGG AGCTGCTGCT CAGGGCCCGC
    GGGGAGCTGG ACCGGCAGCT GCGGGAGCAC CGGCAGGACC TGGAGCGGCT GCGGGAGGGC
    CAGCGCCTGG TGGAGAGTGC GAGGGCCCGC ACGCGCGCCC AGCACAGCCT GCTGCGTGGC
    CCGAGGCCCG GGCGCCAGAG CAGCCTGCCC GCGGCCTTGC CTCCGGGCAG CGGCGAGGTG
    ATGGAACTTA ATCGATCCGA GAGCTTGTGT CATGAAAATT CATTCTTCAT TAATGAAGCT
    TTAGTACAAA TGTCACTTAA CACTTACAAC AAATCAACTC CATCAGGTGT CCACCAGGAA
    GCCGCTTATC CCCAGAATAT AGCTAATTCG GACTTGGTAA GGACTAGTGA AAGTCCCGTA
    GACCTCCAGT TGGACATTTC TCAGCCCTTG GATGTCAACC ACGAACTGTG GACAGCCGCT
    GGGTCCTGTC ATCAGATACC CCCTCTCCAC GAAAGCAGCA AGGATTCTTG TAAAAATGGA
    AGAATTTACA TCAGTCAGCT ATGTTTCCAG AGCCATTCGT CAGAAATGTT TTGGCGACCA
    GACTTGACCA TCTCAGCTTG GGATAGAGAG CTTCCGAATC TGCAGAACTT CCAACCGCTG
    TTGGTTGCAT TATGTAGATG GTTGCGTTAC GTCCGTACAT CCCCGGGAGA TCTTGCCGTC
    ATCCTCGAGT CCCCTGAGCA GGTTTTTCAG CTAGAAAGAA AATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005617528.1
    CDS92..5239
    Translation

    Target ORF information:

    RefSeq Version XM_005617471.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005617471.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGTTGA GCTGCAGCGA AGTACCTCTT TATGGGCAGA TGACCGTCTA TGCAAAGTTT 
    GGGAAAAATG TTTATCTTCC TAAAGATGCC GAGTTTTACT TTATTTATAA TGGATCTCAT
    CAGAGACACG TCGTGATTGC AAAACGCACT GAGGATAATG TTCTCCAGTC CAGCATTCCA
    GGGCATGGGC TTCAGGAGAC GGTGACGGTG TCTGTATGCC TCTGCTCAGA AGGTTACTCC
    CCAGTGACCA TGGGCTCCGG CTCAGTGACC TACGTGGACA ACATGGCTTG CAGGCTGGCT
    CGTTTGCTGG TCACCCAGGC TGATCGCCTC ACAGCCAGTA GTCATCAGAC CTTTCTGACT
    CCATTTGCCC TGACGGTGGG AGCATTGCCT GCTTTGGACG AGGAGCTGGT GCTGGCTCTG
    ACCCATCTGG AGTTGCCTCT TGAGTGGAGT GTCTTGGGAA ATTCTTCACT TGAAGTTTCT
    TTATGCAGAG AGTCTCTTCT ACACCTGGCT ATGAGATGGG GCCTGCCTAA GCTCTCTCAT
    TTCCTCTTGT GCCTCCCTGG GGGAGTTCAG GCTTTGTCTT TACCCAATGA AGAAGGTGCC
    ACACCATTAG ACTTGGCTTT ACGTGCCGGA CACTCCAAGC TGGTTGAAGA CATCACAAAT
    TTCCAGGGCA GACGTTCCCC CGACCTCTCC CGAGTGCACC TCGGCGGACG TGCCTTCCTG
    CAGTATGTTC ACCCGTCGGG GACACTGACG CTGACGCTGA ACCACACAGC TGAGCATTTG
    TTGGAGGCAG ATATTAAACT CTTCAGGAAA TACTTTTTGG ACAGAGCCTT TCTCCTCAAG
    GCTCTTGACG ACCGAGAAGC TGGGCCAGAG GAGAGCCGGG GCATGCCCTC CCGGGCTGCC
    GCCACTGAGG AGGAGATTAG GAATTTGGTG ACCGGCAGGC CATCACCTGA AAAGGAGGAT
    GTAAAGCGTG TGGACAGCCC GGACGTTGAC CCCCATGAAC ACGACGGCCG GGACAGTCTT
    GATTTGGATC ACTCTTTCGA TGTCTTCAAA AAACCTAAGC ATCCCCCAGC GTTCTTTGCT
    GCAGGCGGGC TCTCTGATGT GCTGAATGGC GGCGATGAAG TTTACGCCAA CTGTATGGTG
    ATTGACCAGG TTGGTGATTT GGATATTAAC TACATCAATT TAGAAGGAAT CTCTACAGAC
    GCCTGTGCTG AATCCATGGG TTCTACTCTG GGGCCTCAGA ACTGCGAGCA TATCCTTGCT
    GCTGAAACCA GCCATGGGAA ATACCCACTA AGTGAGAACA AGGAAGTGGT CTCAGATACC
    GAAGCTCAGC AGTCCTTCCC ATCACCGTCT AGTTCATATG CTTCCAATTT TGATCTTTCC
    TTTGGTCTTC ATGGATTTGA GAAGGAACAA AGTCTCCTGA AGAAAAGAAG CTCCAGCCTG
    GATGCCTTGG ATGCCGACAG CGAAGGGGAA GGGCATTCTG AGCGGTCACA CATCTGCTAC
    ACGCCAGCAT CTCAGAGTTC CTCGAGGACT GGGATTCCCA GTGGGGATGA GCTGGACTCC
    TTCGAGACCG CCACCAAACC TGATTTTAGT ATCTCCAGGA CAGAGTCGCT TTCTTTATCA
    AGTAGCCTGC AGTTGAAGGA ATCATTGCTT CCTGGAGTCC GCTCACGTTC TTATTCCTGC
    TCATCACCCA AAATTTCTTT AGGAAAAACT CGATTGGTGC GTGATTTCAC AGTGTGCAAT
    TCTGGTGAAG AGCAAAGAGC CTTCGGCTTC CCGGAGCCCG CCAGGGAGAG GAGGATTCAA
    GAGGAAGAAT GGGATAAATA CATCATACCT GCCAAGTCAG AGTCTGAGAA ATACAAAGTG
    AGCCGGACCT TCAGCTTCCT CATGAACAGG ATGACAAGCC CTCGGAACAA ATCTAAGACG
    AAAAGCAAGG ATGCCAAAGA CAAAGAGAAA CCGAGCCGAC ATCAGTTTGT CCCCGGAACC
    TTCTCTGGGG TTCTCCAGTG TTCCGTTTGT GATAAAACGC TCCTGGGGAA GGAGTCATCC
    CAGTGTTCCA ACTGTAATGC AAACGTCCAC AAGGGTTGTA AGGACGCTGC ACCTCCGTGT
    ACCAAGAAAT TCCAGGAGAA ATATAACAAG AACAAACCGC AGACCATCCT TGGAAACTCT
    TCATTTCGAG ACATCCCGCA ACCCAGTCTC TCCTTGCACC CTTCTTCCTC CATGCCTATC
    GGATTGCCCA CTGGGAGAAA AGAAGCTTCT GCCCAGGCCC ATCCCTTGTC CAGAAGTGTT
    CCAGGCACTG CCTTGGAAAG CTTCAGAAGA TCGGGGCTAT CCCTGGAGTC TGAGAGTGAC
    GGGAACAGCT GGAGAAGCAG GTCTCATTCC GATGAGCTGT TACAGTCCAT GGGCTCTTCT
    CCCTCCACGG AATCCTTCTT AATGGAAGAT GTCGTGGACT CTTCTCTATG GAGTGACCTC
    AGCAGTGACG CCCAGGAGTT GGAAGCAGAA TCCTGGAGTC TGGTGGTGGA CCCCTCCTTT
    TGCAATAAGC AGGAGAAAGA TGTCATCAAA AGACAGGATG TCATTTTCGA GCTTATGCAA
    ACAGAGATGC ATCATATCCA GACCCTGCTC ATCATGTCTG AGATCTTCAG GAAAGGAATG
    AAGGAGGAGC TGCAGCTTGA TCACAGCACC GTGGATAAAA TCTTTCCCTG CTTAGATGAG
    TTACTTGAAA TCCACAGGCA TTTCTTTTAT AGTATGAAGG AGCGAAGGCA GGAGTCCTGT
    GCTGGTGGTG ACAGGAATTT TGTCATCAGC CGAATTGGAG ATATTCTAGT ACAGCAGTTT
    TCAGAAGAAA ATGCAAATCA AATGAAGAAA ATATATGGAG AATTCTGTAG CCATCACAAA
    GAAGCTGTTA GCCTCTATAA AGAACTCCAA CAGAATAAGA AGTTTCAGAA TTTTATTAAG
    CTTCGAAATA GTAATCTTTT GGCTCGACGC CGAGGAATCC CAGAATGCAT TCTGCTAGTC
    ACTCAGCGCA TCACAAAATA CCCTGTCCTG GTGGAACGGA TTTTGCAGTA CACAAAGGAA
    AGAAGTGAAG AACATCAAGA CTTGTGCAAA GCTCTGTGCT TAATTAAAGA CATGATTGCA
    GCAGTGGATT TAAAAGTCAG CGAGTATGAG AAAAACCAAA AATGGCTTGA GATCCTAAAT
    AAGATCGAAA ACAAAACCTA CACAAAGCTC AAAAATGGCC ACGTGTTTAG GAAGCAAGCA
    CTGATAAGTA AAGAAAGGAC TCTCTTATAT GATGGCCTTG TTTATTGGAA AACTGCTACG
    GGTCGTTTCA AAGATATCCT GGCTCTACTT CTAACTGATG TGCTGCTCTT TTTACAAGAA
    AAAGACCAGA AATACATCTT TGCAGCTGTT GATCAGAAGC CATCGGTTAT TTCCCTTCAG
    AAGCTCATTG CTAGAGAAGT CGCCAACGAG GAAAGAGGAA TGTTCCTGAT CAGTGCATCC
    TCTGCTGGTC CTGAGATGTA CGAAATTCAC ACCAACTCCA AGGAGGAACG AAATAACTGG
    ATGAGACGAA TCCAACAGGC CGTGGAAAGT TGCCCAGAAG AAGAAGGGGG AAGGACAAGT
    GAATCTGATG AAGAGAGGCG GAAAGCTGAA GCAAGAGTGG CCAAAATCCA GCAATGTCAA
    GAAATACTCA GTAACCAAGA CCAACAGATT TGCGCATATT TGGAGGAGAA GCTGCATATC
    TACGCTGAAC TTGGAGAACT GAGTGGATTC AAGGATGTCC ATCTAGAGCC CCACCTCCTC
    ATCAAACCTG ACCCAGGGGA GCCTCCCCAG GCAGCCTCAT TACTAGCAGC AGCCCTGAGA
    GAAGCGGAGA GCCTGCAGGT TGCCGTGAAG GCCTCACAAG CGGGTGATGC TCGCCCATCA
    CCTGAGGAGG GTTCTGCAGA ACCTGCGGTT GCAGATGCAC AGGGTCCTCC CGAGGAGCCA
    GGATCATCTC CAGCCTCACT AGTCACAGAA GGGACAGGAG GAAGAGGCTG TTGGGATGTG
    GATCCTGGCC CCCAGGGTGT GGGAACCGAC TTGGCGGTCT CTGATGCAGG GGAGAAGGTG
    GAATATAGAA GTTTCACGGT TTCTTCACAG TCAGAGATTA TACAAGCGAT CCAGAATTTA
    ACCCGACTCC TCTATAGCCT TCAGGCCGCG CTGACCATCC AGGACAGCCA CATCCAGGTG
    CACAGGCTGC TGCTGCAGCA GGCTGGCCTG CCGGCGTCCC AAGAGGAGCA CGGCGAGCTG
    GCGAGGCAGC GGGAGGAGCT GGCCGCCCTG CCCGGGCTCC AGTGGCAGCT GCAGCAGGAG
    CGGCGGCGCG GCCAGCGCAC CTGGGAGCGG CAGCAGCGCG AGCAGGAGGC CAGGGCCTGC
    CGGCTGCGGG AGCGCGAGCA GGGCTGCCGC GGCCGCGAGG AGCTGCTGCT CAGGGCCCGC
    GGGGAGCTGG ACCGGCAGCT GCGGGAGCAC CGGCAGGACC TGGAGCGGCT GCGGGAGGGC
    CAGCGCCTGG TGGAGAGTGC GAGGGCCCGC ACGCGCGCCC AGCACAGCCT GCTGCGTGGC
    CCGAGGCCCG GGCGCCAGAG CAGCCTGCCC GCGGCCTTGC CTCCGGGCAG CGGCGAGGTG
    ATGGAACTTA ATCGATCCGA GAGCTTGTGT CATGAAAATT CATTCTTCAT TAATGAAGCT
    TTAGTACAAA TGTCACTTAA CACTTACAAC AAATCAACTC CATCAGGTGT CCACCAGGAA
    GCCGCTTATC CCCAGAATAT AGCTAATTCG GACTTGGTAA GGACTAGTGA AAGTCCCGTA
    GACCTCCAGT TGGACATTTC TCAGCCCTTG GATGTCAACC ACGAACTGTG GACAGCCGCT
    GGGTCCTGTC ATCAGATACC CCCTCTCCAC GAAAGCAGCA AGGATTCTTG TAAAAATGGA
    AGAATTTACA TCAGTCAGCT ATGTTTCCAG AGCCATTCGT CAGAAATGTT TTGGCGACCA
    GACTTGACCA TCTCAGCTTG GGATAGAGAG CTTCCGAATC TGCAGAACTT CCAACCGCTG
    TTGGTTGCAT TATGTAGATG GTTGCGTTAC GTCCGTACAT CCCCGGGAGA TCTTGCCGTC
    ATCCTCGAGT CCCCTGAGCA GGTTTTTCAG CTAGAAAGAA AATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03225
    Clone ID Related Accession (Same CDS sequence) XM_005617476.2 , XM_005617476.3
    Accession Version XM_005617476.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4206bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product rho guanine nucleotide exchange factor 28 isoform X10
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005617476.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGACTCGG ACTCGGACTC CCCTTTGAAC TACTCGTGGC CGTCCTTCCC CAAGATGAAG 
    ACGCGCCGGA GGGCGGCCAA GCCAGATCAC TCTTTCGATG TCTTCAAAAA ACCTAAGCAT
    CCCCCAGCGT TCTTTGCTGC AGGCGGGCTC TCTGATGTGC TGAATGGCGG CGATGAAGTT
    TACGCCAACT GTATGGTGAT TGACCAGGTT GGTGATTTGG ATATTAACTA CATCAATTTA
    GAAGGAATCT CTACAGACGC CTGTGCTGAA TCCATGGGTT CTACTCTGGG GCCTCAGAAC
    TGCGAGCATA TCCTTGCTGC TGAAACCAGC CATGGGAAAT ACCCACTAAG TGAGAACAAG
    GAAGTGGTCT CAGATACCGA AGCTCAGCAG TCCTTCCCAT CACCGTCTAG TTCATATGCT
    TCCAATTTTG ATCTTTCCTT TGGTCTTCAT GGATTTGAGA AGGAACAAAG TCTCCTGAAG
    AAAAGAAGCT CCAGCCTGGA TGCCTTGGAT GCCGACAGCG AAGGGGAAGG GCATTCTGAG
    CGGTCACACA TCTGCTACAC GCCAGCATCT CAGAGTTCCT CGAGGACTGG GATTCCCAGT
    GGGGATGAGC TGGACTCCTT CGAGACCGCC ACCAAACCTG ATTTTAGTAT CTCCAGGACA
    GAGTCGCTTT CTTTATCAAG TAGCCTGCAG TTGAAGGAAT CATTGCTTCC TGGAGTCCGC
    TCACGTTCTT ATTCCTGCTC ATCACCCAAA ATTTCTTTAG GAAAAACTCG ATTGGTGCGT
    GATTTCACAG TGTGCAATTC TGGTGAAGAG CAAAGAGCCT TCGGCTTCCC GGAGCCCGCC
    AGGGAGAGGA GGATTCAAGA GGAAGAATGG GATAAATACA TCATACCTGC CAAGTCAGAG
    TCTGAGAAAT ACAAAGTGAG CCGGACCTTC AGCTTCCTCA TGAACAGGAT GACAAGCCCT
    CGGAACAAAT CTAAGACGAA AAGCAAGGAT GCCAAAGACA AAGAGAAACC GAGCCGACAT
    CAGTTTGTCC CCGGAACCTT CTCTGGGGTT CTCCAGTGTT CCGTTTGTGA TAAAACGCTC
    CTGGGGAAGG AGTCATCCCA GTGTTCCAAC TGTAATGCAA ACGTCCACAA GGGTTGTAAG
    GACGCTGCAC CTCCGTGTAC CAAGAAATTC CAGGAGAAAT ATAACAAGAA CAAACCGCAG
    ACCATCCTTG GAAACTCTTC ATTTCGAGAC ATCCCGCAAC CCAGTCTCTC CTTGCACCCT
    TCTTCCTCCA TGCCTATCGG ATTGCCCACT GGGAGAAAAG AAGCTTCTGC CCAGGCCCAT
    CCCTTGTCCA GAAGTGTTCC AGGCACTGCC TTGGAAAGCT TCAGAAGATC GGGGCTATCC
    CTGGAGTCTG AGAGTGACGG GAACAGCTGG AGAAGCAGGT CTCATTCCGA TGAGCTGTTA
    CAGTCCATGG GCTCTTCTCC CTCCACGGAA TCCTTCTTAA TGGAAGATGT CGTGGACTCT
    TCTCTATGGA GTGACCTCAG CAGTGACGCC CAGGAGTTGG AAGCAGAATC CTGGAGTCTG
    GTGGTGGACC CCTCCTTTTG CAATAAGCAG GAGAAAGATG TCATCAAAAG ACAGGATGTC
    ATTTTCGAGC TTATGCAAAC AGAGATGCAT CATATCCAGA CCCTGCTCAT CATGTCTGAG
    ATCTTCAGGA AAGGAATGAA GGAGGAGCTG CAGCTTGATC ACAGCACCGT GGATAAAATC
    TTTCCCTGCT TAGATGAGTT ACTTGAAATC CACAGGCATT TCTTTTATAG TATGAAGGAG
    CGAAGGCAGG AGTCCTGTGC TGGTGGTGAC AGGAATTTTG TCATCAGCCG AATTGGAGAT
    ATTCTAGTAC AGCAGTTTTC AGAAGAAAAT GCAAATCAAA TGAAGAAAAT ATATGGAGAA
    TTCTGTAGCC ATCACAAAGA AGCTGTTAGC CTCTATAAAG AACTCCAACA GAATAAGAAG
    TTTCAGAATT TTATTAAGCT TCGAAATAGT AATCTTTTGG CTCGACGCCG AGGAATCCCA
    GAATGCATTC TGCTAGTCAC TCAGCGCATC ACAAAATACC CTGTCCTGGT GGAACGGATT
    TTGCAGTACA CAAAGGAAAG AAGTGAAGAA CATCAAGACT TGTGCAAAGC TCTGTGCTTA
    ATTAAAGACA TGATTGCAGC AGTGGATTTA AAAGTCAGCG AGTATGAGAA AAACCAAAAA
    TGGCTTGAGA TCCTAAATAA GATCGAAAAC AAAACCTACA CAAAGCTCAA AAATGGCCAC
    GTGTTTAGGA AGCAAGCACT GATAAGTAAA GAAAGGACTC TCTTATATGA TGGCCTTGTT
    TATTGGAAAA CTGCTACGGG TCGTTTCAAA GATATCCTGG CTCTACTTCT AACTGATGTG
    CTGCTCTTTT TACAAGAAAA AGACCAGAAA TACATCTTTG CAGCTGTTGA TCAGAAGCCA
    TCGGTTATTT CCCTTCAGAA GCTCATTGCT AGAGAAGTCG CCAACGAGGA AAGAGGAATG
    TTCCTGATCA GTGCATCCTC TGCTGGTCCT GAGATGTACG AAATTCACAC CAACTCCAAG
    GAGGAACGAA ATAACTGGAT GAGACGAATC CAACAGGCCG TGGAAAGTTG CCCAGAAGAA
    GAAGGGGGAA GGACAAGTGA ATCTGATGAA GAGAGGCGGA AAGCTGAAGC AAGAGTGGCC
    AAAATCCAGC AATGTCAAGA AATACTCAGT AACCAAGACC AACAGATTTG CGCATATTTG
    GAGGAGAAGC TGCATATCTA CGCTGAACTT GGAGAACTGA GTGGATTCAA GGATGTCCAT
    CTAGAGCCCC ACCTCCTCAT CAAACCTGAC CCAGGGGAGC CTCCCCAGGC AGCCTCATTA
    CTAGCAGCAG CCCTGAGAGA AGCGGAGAGC CTGCAGGTTG CCGTGAAGGC CTCACAAGCG
    GGTGATGCTC GCCCATCACC TGAGGAGGGT TCTGCAGAAC CTGCGGTTGC AGATGCACAG
    GGTCCTCCCG AGGAGCCAGG ATCATCTCCA GCCTCACTAG TCACAGAAGG GACAGGAGGA
    AGAGGCTGTT GGGATGTGGA TCCTGGCCCC CAGGGTGTGG GAACCGACTT GGCGGTCTCT
    GATGCAGGGG AGAAGGTGGA ATATAGAAGT TTCACGGTTT CTTCACAGTC AGAGATTATA
    CAAGCGATCC AGAATTTAAC CCGACTCCTC TATAGCCTTC AGGCCGCGCT GACCATCCAG
    GACAGCCACA TCCAGGTGCA CAGGCTGCTG CTGCAGCAGG CTGGCCTGCC GGCGTCCCAA
    GAGGAGCACG GCGAGCTGGC GAGGCAGCGG GAGGAGCTGG CCGCCCTGCC CGGGCTCCAG
    TGGCAGCTGC AGCAGGAGCG GCGGCGCGGC CAGCGCACCT GGGAGCGGCA GCAGCGCGAG
    CAGGAGGCCA GGGCCTGCCG GCTGCGGGAG CGCGAGCAGG GCTGCCGCGG CCGCGAGGAG
    CTGCTGCTCA GGGCCCGCGG GGAGCTGGAC CGGCAGCTGC GGGAGCACCG GCAGGACCTG
    GAGCGGCTGC GGGAGGGCCA GCGCCTGGTG GAGAGTGCGA GGGCCCGCAC GCGCGCCCAG
    CACAGCCTGC TGCGTGGCCC GAGGCCCGGG CGCCAGAGCA GCCTGCCCGC GGCCTTGCCT
    CCGGGCAGCG GCGAGGTGAT GGAACTTAAT CGATCCGAGA GCTTGTGTCA TGAAAATTCA
    TTCTTCATTA ATGAAGCTTT AGTACAAATG TCACTTAACA CTTACAACAA ATCAACTCCA
    TCAGGTGTCC ACCAGGAAGC CGCTTATCCC CAGAATATAG CTAATTCGGA CTTGGTAAGG
    ACTAGTGAAA GTCCCGTAGA CCTCCAGTTG GACATTTCTC AGCCCTTGGA TGTCAACCAC
    GAACTGTGGA CAGCCGCTGG GTCCTGTCAT CAGATACCCC CTCTCCACGA AAGCAGCAAG
    GATTCTTGTA AAAATGGAAG AATTTACATC AGTCAGCTAT GTTTCCAGAG CCATTCGTCA
    GAAATGTTTT GGCGACCAGA CTTGACCATC TCAGCTTGGG ATAGAGAGCT TCCGAATCTG
    CAGAACTTCC AACCGCTGTT GGTTGCATTA TGTAGATGGT TGCGTTACGT CCGTACATCC
    CCGGGAGATC TTGCCGTCAT CCTCGAGTCC CCTGAGCAGG TTTTTCAGCT AGAAAGAAAA
    TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005617533.1
    CDS98..4303
    Translation

    Target ORF information:

    RefSeq Version XM_005617476.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 28 (ARHGEF28), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005617476.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGACTCGG ACTCGGACTC CCCTTTGAAC TACTCGTGGC CGTCCTTCCC CAAGATGAAG 
    ACGCGCCGGA GGGCGGCCAA GCCAGATCAC TCTTTCGATG TCTTCAAAAA ACCTAAGCAT
    CCCCCAGCGT TCTTTGCTGC AGGCGGGCTC TCTGATGTGC TGAATGGCGG CGATGAAGTT
    TACGCCAACT GTATGGTGAT TGACCAGGTT GGTGATTTGG ATATTAACTA CATCAATTTA
    GAAGGAATCT CTACAGACGC CTGTGCTGAA TCCATGGGTT CTACTCTGGG GCCTCAGAAC
    TGCGAGCATA TCCTTGCTGC TGAAACCAGC CATGGGAAAT ACCCACTAAG TGAGAACAAG
    GAAGTGGTCT CAGATACCGA AGCTCAGCAG TCCTTCCCAT CACCGTCTAG TTCATATGCT
    TCCAATTTTG ATCTTTCCTT TGGTCTTCAT GGATTTGAGA AGGAACAAAG TCTCCTGAAG
    AAAAGAAGCT CCAGCCTGGA TGCCTTGGAT GCCGACAGCG AAGGGGAAGG GCATTCTGAG
    CGGTCACACA TCTGCTACAC GCCAGCATCT CAGAGTTCCT CGAGGACTGG GATTCCCAGT
    GGGGATGAGC TGGACTCCTT CGAGACCGCC ACCAAACCTG ATTTTAGTAT CTCCAGGACA
    GAGTCGCTTT CTTTATCAAG TAGCCTGCAG TTGAAGGAAT CATTGCTTCC TGGAGTCCGC
    TCACGTTCTT ATTCCTGCTC ATCACCCAAA ATTTCTTTAG GAAAAACTCG ATTGGTGCGT
    GATTTCACAG TGTGCAATTC TGGTGAAGAG CAAAGAGCCT TCGGCTTCCC GGAGCCCGCC
    AGGGAGAGGA GGATTCAAGA GGAAGAATGG GATAAATACA TCATACCTGC CAAGTCAGAG
    TCTGAGAAAT ACAAAGTGAG CCGGACCTTC AGCTTCCTCA TGAACAGGAT GACAAGCCCT
    CGGAACAAAT CTAAGACGAA AAGCAAGGAT GCCAAAGACA AAGAGAAACC GAGCCGACAT
    CAGTTTGTCC CCGGAACCTT CTCTGGGGTT CTCCAGTGTT CCGTTTGTGA TAAAACGCTC
    CTGGGGAAGG AGTCATCCCA GTGTTCCAAC TGTAATGCAA ACGTCCACAA GGGTTGTAAG
    GACGCTGCAC CTCCGTGTAC CAAGAAATTC CAGGAGAAAT ATAACAAGAA CAAACCGCAG
    ACCATCCTTG GAAACTCTTC ATTTCGAGAC ATCCCGCAAC CCAGTCTCTC CTTGCACCCT
    TCTTCCTCCA TGCCTATCGG ATTGCCCACT GGGAGAAAAG AAGCTTCTGC CCAGGCCCAT
    CCCTTGTCCA GAAGTGTTCC AGGCACTGCC TTGGAAAGCT TCAGAAGATC GGGGCTATCC
    CTGGAGTCTG AGAGTGACGG GAACAGCTGG AGAAGCAGGT CTCATTCCGA TGAGCTGTTA
    CAGTCCATGG GCTCTTCTCC CTCCACGGAA TCCTTCTTAA TGGAAGATGT CGTGGACTCT
    TCTCTATGGA GTGACCTCAG CAGTGACGCC CAGGAGTTGG AAGCAGAATC CTGGAGTCTG
    GTGGTGGACC CCTCCTTTTG CAATAAGCAG GAGAAAGATG TCATCAAAAG ACAGGATGTC
    ATTTTCGAGC TTATGCAAAC AGAGATGCAT CATATCCAGA CCCTGCTCAT CATGTCTGAG
    ATCTTCAGGA AAGGAATGAA GGAGGAGCTG CAGCTTGATC ACAGCACCGT GGATAAAATC
    TTTCCCTGCT TAGATGAGTT ACTTGAAATC CACAGGCATT TCTTTTATAG TATGAAGGAG
    CGAAGGCAGG AGTCCTGTGC TGGTGGTGAC AGGAATTTTG TCATCAGCCG AATTGGAGAT
    ATTCTAGTAC AGCAGTTTTC AGAAGAAAAT GCAAATCAAA TGAAGAAAAT ATATGGAGAA
    TTCTGTAGCC ATCACAAAGA AGCTGTTAGC CTCTATAAAG AACTCCAACA GAATAAGAAG
    TTTCAGAATT TTATTAAGCT TCGAAATAGT AATCTTTTGG CTCGACGCCG AGGAATCCCA
    GAATGCATTC TGCTAGTCAC TCAGCGCATC ACAAAATACC CTGTCCTGGT GGAACGGATT
    TTGCAGTACA CAAAGGAAAG AAGTGAAGAA CATCAAGACT TGTGCAAAGC TCTGTGCTTA
    ATTAAAGACA TGATTGCAGC AGTGGATTTA AAAGTCAGCG AGTATGAGAA AAACCAAAAA
    TGGCTTGAGA TCCTAAATAA GATCGAAAAC AAAACCTACA CAAAGCTCAA AAATGGCCAC
    GTGTTTAGGA AGCAAGCACT GATAAGTAAA GAAAGGACTC TCTTATATGA TGGCCTTGTT
    TATTGGAAAA CTGCTACGGG TCGTTTCAAA GATATCCTGG CTCTACTTCT AACTGATGTG
    CTGCTCTTTT TACAAGAAAA AGACCAGAAA TACATCTTTG CAGCTGTTGA TCAGAAGCCA
    TCGGTTATTT CCCTTCAGAA GCTCATTGCT AGAGAAGTCG CCAACGAGGA AAGAGGAATG
    TTCCTGATCA GTGCATCCTC TGCTGGTCCT GAGATGTACG AAATTCACAC CAACTCCAAG
    GAGGAACGAA ATAACTGGAT GAGACGAATC CAACAGGCCG TGGAAAGTTG CCCAGAAGAA
    GAAGGGGGAA GGACAAGTGA ATCTGATGAA GAGAGGCGGA AAGCTGAAGC AAGAGTGGCC
    AAAATCCAGC AATGTCAAGA AATACTCAGT AACCAAGACC AACAGATTTG CGCATATTTG
    GAGGAGAAGC TGCATATCTA CGCTGAACTT GGAGAACTGA GTGGATTCAA GGATGTCCAT
    CTAGAGCCCC ACCTCCTCAT CAAACCTGAC CCAGGGGAGC CTCCCCAGGC AGCCTCATTA
    CTAGCAGCAG CCCTGAGAGA AGCGGAGAGC CTGCAGGTTG CCGTGAAGGC CTCACAAGCG
    GGTGATGCTC GCCCATCACC TGAGGAGGGT TCTGCAGAAC CTGCGGTTGC AGATGCACAG
    GGTCCTCCCG AGGAGCCAGG ATCATCTCCA GCCTCACTAG TCACAGAAGG GACAGGAGGA
    AGAGGCTGTT GGGATGTGGA TCCTGGCCCC CAGGGTGTGG GAACCGACTT GGCGGTCTCT
    GATGCAGGGG AGAAGGTGGA ATATAGAAGT TTCACGGTTT CTTCACAGTC AGAGATTATA
    CAAGCGATCC AGAATTTAAC CCGACTCCTC TATAGCCTTC AGGCCGCGCT GACCATCCAG
    GACAGCCACA TCCAGGTGCA CAGGCTGCTG CTGCAGCAGG CTGGCCTGCC GGCGTCCCAA
    GAGGAGCACG GCGAGCTGGC GAGGCAGCGG GAGGAGCTGG CCGCCCTGCC CGGGCTCCAG
    TGGCAGCTGC AGCAGGAGCG GCGGCGCGGC CAGCGCACCT GGGAGCGGCA GCAGCGCGAG
    CAGGAGGCCA GGGCCTGCCG GCTGCGGGAG CGCGAGCAGG GCTGCCGCGG CCGCGAGGAG
    CTGCTGCTCA GGGCCCGCGG GGAGCTGGAC CGGCAGCTGC GGGAGCACCG GCAGGACCTG
    GAGCGGCTGC GGGAGGGCCA GCGCCTGGTG GAGAGTGCGA GGGCCCGCAC GCGCGCCCAG
    CACAGCCTGC TGCGTGGCCC GAGGCCCGGG CGCCAGAGCA GCCTGCCCGC GGCCTTGCCT
    CCGGGCAGCG GCGAGGTGAT GGAACTTAAT CGATCCGAGA GCTTGTGTCA TGAAAATTCA
    TTCTTCATTA ATGAAGCTTT AGTACAAATG TCACTTAACA CTTACAACAA ATCAACTCCA
    TCAGGTGTCC ACCAGGAAGC CGCTTATCCC CAGAATATAG CTAATTCGGA CTTGGTAAGG
    ACTAGTGAAA GTCCCGTAGA CCTCCAGTTG GACATTTCTC AGCCCTTGGA TGTCAACCAC
    GAACTGTGGA CAGCCGCTGG GTCCTGTCAT CAGATACCCC CTCTCCACGA AAGCAGCAAG
    GATTCTTGTA AAAATGGAAG AATTTACATC AGTCAGCTAT GTTTCCAGAG CCATTCGTCA
    GAAATGTTTT GGCGACCAGA CTTGACCATC TCAGCTTGGG ATAGAGAGCT TCCGAATCTG
    CAGAACTTCC AACCGCTGTT GGTTGCATTA TGTAGATGGT TGCGTTACGT CCGTACATCC
    CCGGGAGATC TTGCCGTCAT CCTCGAGTCC CCTGAGCAGG TTTTTCAGCT AGAAAGAAAA
    TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03225
    Clone ID Related Accession (Same CDS sequence) XM_005617476.2 , XM_005617476.3
    Accession Version XM_005617476.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4206bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rho guanine nucleotide exchange factor 28 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005617476.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGACTCGG ACTCGGACTC CCCTTTGAAC TACTCGTGGC CGTCCTTCCC CAAGATGAAG 
    ACGCGCCGGA GGGCGGCCAA GCCAGATCAC TCTTTCGATG TCTTCAAAAA ACCTAAGCAT
    CCCCCAGCGT TCTTTGCTGC AGGCGGGCTC TCTGATGTGC TGAATGGCGG CGATGAAGTT
    TACGCCAACT GTATGGTGAT TGACCAGGTT GGTGATTTGG ATATTAACTA CATCAATTTA
    GAAGGAATCT CTACAGACGC CTGTGCTGAA TCCATGGGTT CTACTCTGGG GCCTCAGAAC
    TGCGAGCATA TCCTTGCTGC TGAAACCAGC CATGGGAAAT ACCCACTAAG TGAGAACAAG
    GAAGTGGTCT CAGATACCGA AGCTCAGCAG TCCTTCCCAT CACCGTCTAG TTCATATGCT
    TCCAATTTTG ATCTTTCCTT TGGTCTTCAT GGATTTGAGA AGGAACAAAG TCTCCTGAAG
    AAAAGAAGCT CCAGCCTGGA TGCCTTGGAT GCCGACAGCG AAGGGGAAGG GCATTCTGAG
    CGGTCACACA TCTGCTACAC GCCAGCATCT CAGAGTTCCT CGAGGACTGG GATTCCCAGT
    GGGGATGAGC TGGACTCCTT CGAGACCGCC ACCAAACCTG ATTTTAGTAT CTCCAGGACA
    GAGTCGCTTT CTTTATCAAG TAGCCTGCAG TTGAAGGAAT CATTGCTTCC TGGAGTCCGC
    TCACGTTCTT ATTCCTGCTC ATCACCCAAA ATTTCTTTAG GAAAAACTCG ATTGGTGCGT
    GATTTCACAG TGTGCAATTC TGGTGAAGAG CAAAGAGCCT TCGGCTTCCC GGAGCCCGCC
    AGGGAGAGGA GGATTCAAGA GGAAGAATGG GATAAATACA TCATACCTGC CAAGTCAGAG
    TCTGAGAAAT ACAAAGTGAG CCGGACCTTC AGCTTCCTCA TGAACAGGAT GACAAGCCCT
    CGGAACAAAT CTAAGACGAA AAGCAAGGAT GCCAAAGACA AAGAGAAACC GAGCCGACAT
    CAGTTTGTCC CCGGAACCTT CTCTGGGGTT CTCCAGTGTT CCGTTTGTGA TAAAACGCTC
    CTGGGGAAGG AGTCATCCCA GTGTTCCAAC TGTAATGCAA ACGTCCACAA GGGTTGTAAG
    GACGCTGCAC CTCCGTGTAC CAAGAAATTC CAGGAGAAAT ATAACAAGAA CAAACCGCAG
    ACCATCCTTG GAAACTCTTC ATTTCGAGAC ATCCCGCAAC CCAGTCTCTC CTTGCACCCT
    TCTTCCTCCA TGCCTATCGG ATTGCCCACT GGGAGAAAAG AAGCTTCTGC CCAGGCCCAT
    CCCTTGTCCA GAAGTGTTCC AGGCACTGCC TTGGAAAGCT TCAGAAGATC GGGGCTATCC
    CTGGAGTCTG AGAGTGACGG GAACAGCTGG AGAAGCAGGT CTCATTCCGA TGAGCTGTTA
    CAGTCCATGG GCTCTTCTCC CTCCACGGAA TCCTTCTTAA TGGAAGATGT CGTGGACTCT
    TCTCTATGGA GTGACCTCAG CAGTGACGCC CAGGAGTTGG AAGCAGAATC CTGGAGTCTG
    GTGGTGGACC CCTCCTTTTG CAATAAGCAG GAGAAAGATG TCATCAAAAG ACAGGATGTC
    ATTTTCGAGC TTATGCAAAC AGAGATGCAT CATATCCAGA CCCTGCTCAT CATGTCTGAG
    ATCTTCAGGA AAGGAATGAA GGAGGAGCTG CAGCTTGATC ACAGCACCGT GGATAAAATC
    TTTCCCTGCT TAGATGAGTT ACTTGAAATC CACAGGCATT TCTTTTATAG TATGAAGGAG
    CGAAGGCAGG AGTCCTGTGC TGGTGGTGAC AGGAATTTTG TCATCAGCCG AATTGGAGAT
    ATTCTAGTAC AGCAGTTTTC AGAAGAAAAT GCAAATCAAA TGAAGAAAAT ATATGGAGAA
    TTCTGTAGCC ATCACAAAGA AGCTGTTAGC CTCTATAAAG AACTCCAACA GAATAAGAAG
    TTTCAGAATT TTATTAAGCT TCGAAATAGT AATCTTTTGG CTCGACGCCG AGGAATCCCA
    GAATGCATTC TGCTAGTCAC TCAGCGCATC ACAAAATACC CTGTCCTGGT GGAACGGATT
    TTGCAGTACA CAAAGGAAAG AAGTGAAGAA CATCAAGACT TGTGCAAAGC TCTGTGCTTA
    ATTAAAGACA TGATTGCAGC AGTGGATTTA AAAGTCAGCG AGTATGAGAA AAACCAAAAA
    TGGCTTGAGA TCCTAAATAA GATCGAAAAC AAAACCTACA CAAAGCTCAA AAATGGCCAC
    GTGTTTAGGA AGCAAGCACT GATAAGTAAA GAAAGGACTC TCTTATATGA TGGCCTTGTT
    TATTGGAAAA CTGCTACGGG TCGTTTCAAA GATATCCTGG CTCTACTTCT AACTGATGTG
    CTGCTCTTTT TACAAGAAAA AGACCAGAAA TACATCTTTG CAGCTGTTGA TCAGAAGCCA
    TCGGTTATTT CCCTTCAGAA GCTCATTGCT AGAGAAGTCG CCAACGAGGA AAGAGGAATG
    TTCCTGATCA GTGCATCCTC TGCTGGTCCT GAGATGTACG AAATTCACAC CAACTCCAAG
    GAGGAACGAA ATAACTGGAT GAGACGAATC CAACAGGCCG TGGAAAGTTG CCCAGAAGAA
    GAAGGGGGAA GGACAAGTGA ATCTGATGAA GAGAGGCGGA AAGCTGAAGC AAGAGTGGCC
    AAAATCCAGC AATGTCAAGA AATACTCAGT AACCAAGACC AACAGATTTG CGCATATTTG
    GAGGAGAAGC TGCATATCTA CGCTGAACTT GGAGAACTGA GTGGATTCAA GGATGTCCAT
    CTAGAGCCCC ACCTCCTCAT CAAACCTGAC CCAGGGGAGC CTCCCCAGGC AGCCTCATTA
    CTAGCAGCAG CCCTGAGAGA AGCGGAGAGC CTGCAGGTTG CCGTGAAGGC CTCACAAGCG
    GGTGATGCTC GCCCATCACC TGAGGAGGGT TCTGCAGAAC CTGCGGTTGC AGATGCACAG
    GGTCCTCCCG AGGAGCCAGG ATCATCTCCA GCCTCACTAG TCACAGAAGG GACAGGAGGA
    AGAGGCTGTT GGGATGTGGA TCCTGGCCCC CAGGGTGTGG GAACCGACTT GGCGGTCTCT
    GATGCAGGGG AGAAGGTGGA ATATAGAAGT TTCACGGTTT CTTCACAGTC AGAGATTATA
    CAAGCGATCC AGAATTTAAC CCGACTCCTC TATAGCCTTC AGGCCGCGCT GACCATCCAG
    GACAGCCACA TCCAGGTGCA CAGGCTGCTG CTGCAGCAGG CTGGCCTGCC GGCGTCCCAA
    GAGGAGCACG GCGAGCTGGC GAGGCAGCGG GAGGAGCTGG CCGCCCTGCC CGGGCTCCAG
    TGGCAGCTGC AGCAGGAGCG GCGGCGCGGC CAGCGCACCT GGGAGCGGCA GCAGCGCGAG
    CAGGAGGCCA GGGCCTGCCG GCTGCGGGAG CGCGAGCAGG GCTGCCGCGG CCGCGAGGAG
    CTGCTGCTCA GGGCCCGCGG GGAGCTGGAC CGGCAGCTGC GGGAGCACCG GCAGGACCTG
    GAGCGGCTGC GGGAGGGCCA GCGCCTGGTG GAGAGTGCGA GGGCCCGCAC GCGCGCCCAG
    CACAGCCTGC TGCGTGGCCC GAGGCCCGGG CGCCAGAGCA GCCTGCCCGC GGCCTTGCCT
    CCGGGCAGCG GCGAGGTGAT GGAACTTAAT CGATCCGAGA GCTTGTGTCA TGAAAATTCA
    TTCTTCATTA ATGAAGCTTT AGTACAAATG TCACTTAACA CTTACAACAA ATCAACTCCA
    TCAGGTGTCC ACCAGGAAGC CGCTTATCCC CAGAATATAG CTAATTCGGA CTTGGTAAGG
    ACTAGTGAAA GTCCCGTAGA CCTCCAGTTG GACATTTCTC AGCCCTTGGA TGTCAACCAC
    GAACTGTGGA CAGCCGCTGG GTCCTGTCAT CAGATACCCC CTCTCCACGA AAGCAGCAAG
    GATTCTTGTA AAAATGGAAG AATTTACATC AGTCAGCTAT GTTTCCAGAG CCATTCGTCA
    GAAATGTTTT GGCGACCAGA CTTGACCATC TCAGCTTGGG ATAGAGAGCT TCCGAATCTG
    CAGAACTTCC AACCGCTGTT GGTTGCATTA TGTAGATGGT TGCGTTACGT CCGTACATCC
    CCGGGAGATC TTGCCGTCAT CCTCGAGTCC CCTGAGCAGG TTTTTCAGCT AGAAAGAAAA
    TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005617533.1
    CDS107..4312
    Translation

    Target ORF information:

    RefSeq Version XM_005617476.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005617476.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGACTCGG ACTCGGACTC CCCTTTGAAC TACTCGTGGC CGTCCTTCCC CAAGATGAAG 
    ACGCGCCGGA GGGCGGCCAA GCCAGATCAC TCTTTCGATG TCTTCAAAAA ACCTAAGCAT
    CCCCCAGCGT TCTTTGCTGC AGGCGGGCTC TCTGATGTGC TGAATGGCGG CGATGAAGTT
    TACGCCAACT GTATGGTGAT TGACCAGGTT GGTGATTTGG ATATTAACTA CATCAATTTA
    GAAGGAATCT CTACAGACGC CTGTGCTGAA TCCATGGGTT CTACTCTGGG GCCTCAGAAC
    TGCGAGCATA TCCTTGCTGC TGAAACCAGC CATGGGAAAT ACCCACTAAG TGAGAACAAG
    GAAGTGGTCT CAGATACCGA AGCTCAGCAG TCCTTCCCAT CACCGTCTAG TTCATATGCT
    TCCAATTTTG ATCTTTCCTT TGGTCTTCAT GGATTTGAGA AGGAACAAAG TCTCCTGAAG
    AAAAGAAGCT CCAGCCTGGA TGCCTTGGAT GCCGACAGCG AAGGGGAAGG GCATTCTGAG
    CGGTCACACA TCTGCTACAC GCCAGCATCT CAGAGTTCCT CGAGGACTGG GATTCCCAGT
    GGGGATGAGC TGGACTCCTT CGAGACCGCC ACCAAACCTG ATTTTAGTAT CTCCAGGACA
    GAGTCGCTTT CTTTATCAAG TAGCCTGCAG TTGAAGGAAT CATTGCTTCC TGGAGTCCGC
    TCACGTTCTT ATTCCTGCTC ATCACCCAAA ATTTCTTTAG GAAAAACTCG ATTGGTGCGT
    GATTTCACAG TGTGCAATTC TGGTGAAGAG CAAAGAGCCT TCGGCTTCCC GGAGCCCGCC
    AGGGAGAGGA GGATTCAAGA GGAAGAATGG GATAAATACA TCATACCTGC CAAGTCAGAG
    TCTGAGAAAT ACAAAGTGAG CCGGACCTTC AGCTTCCTCA TGAACAGGAT GACAAGCCCT
    CGGAACAAAT CTAAGACGAA AAGCAAGGAT GCCAAAGACA AAGAGAAACC GAGCCGACAT
    CAGTTTGTCC CCGGAACCTT CTCTGGGGTT CTCCAGTGTT CCGTTTGTGA TAAAACGCTC
    CTGGGGAAGG AGTCATCCCA GTGTTCCAAC TGTAATGCAA ACGTCCACAA GGGTTGTAAG
    GACGCTGCAC CTCCGTGTAC CAAGAAATTC CAGGAGAAAT ATAACAAGAA CAAACCGCAG
    ACCATCCTTG GAAACTCTTC ATTTCGAGAC ATCCCGCAAC CCAGTCTCTC CTTGCACCCT
    TCTTCCTCCA TGCCTATCGG ATTGCCCACT GGGAGAAAAG AAGCTTCTGC CCAGGCCCAT
    CCCTTGTCCA GAAGTGTTCC AGGCACTGCC TTGGAAAGCT TCAGAAGATC GGGGCTATCC
    CTGGAGTCTG AGAGTGACGG GAACAGCTGG AGAAGCAGGT CTCATTCCGA TGAGCTGTTA
    CAGTCCATGG GCTCTTCTCC CTCCACGGAA TCCTTCTTAA TGGAAGATGT CGTGGACTCT
    TCTCTATGGA GTGACCTCAG CAGTGACGCC CAGGAGTTGG AAGCAGAATC CTGGAGTCTG
    GTGGTGGACC CCTCCTTTTG CAATAAGCAG GAGAAAGATG TCATCAAAAG ACAGGATGTC
    ATTTTCGAGC TTATGCAAAC AGAGATGCAT CATATCCAGA CCCTGCTCAT CATGTCTGAG
    ATCTTCAGGA AAGGAATGAA GGAGGAGCTG CAGCTTGATC ACAGCACCGT GGATAAAATC
    TTTCCCTGCT TAGATGAGTT ACTTGAAATC CACAGGCATT TCTTTTATAG TATGAAGGAG
    CGAAGGCAGG AGTCCTGTGC TGGTGGTGAC AGGAATTTTG TCATCAGCCG AATTGGAGAT
    ATTCTAGTAC AGCAGTTTTC AGAAGAAAAT GCAAATCAAA TGAAGAAAAT ATATGGAGAA
    TTCTGTAGCC ATCACAAAGA AGCTGTTAGC CTCTATAAAG AACTCCAACA GAATAAGAAG
    TTTCAGAATT TTATTAAGCT TCGAAATAGT AATCTTTTGG CTCGACGCCG AGGAATCCCA
    GAATGCATTC TGCTAGTCAC TCAGCGCATC ACAAAATACC CTGTCCTGGT GGAACGGATT
    TTGCAGTACA CAAAGGAAAG AAGTGAAGAA CATCAAGACT TGTGCAAAGC TCTGTGCTTA
    ATTAAAGACA TGATTGCAGC AGTGGATTTA AAAGTCAGCG AGTATGAGAA AAACCAAAAA
    TGGCTTGAGA TCCTAAATAA GATCGAAAAC AAAACCTACA CAAAGCTCAA AAATGGCCAC
    GTGTTTAGGA AGCAAGCACT GATAAGTAAA GAAAGGACTC TCTTATATGA TGGCCTTGTT
    TATTGGAAAA CTGCTACGGG TCGTTTCAAA GATATCCTGG CTCTACTTCT AACTGATGTG
    CTGCTCTTTT TACAAGAAAA AGACCAGAAA TACATCTTTG CAGCTGTTGA TCAGAAGCCA
    TCGGTTATTT CCCTTCAGAA GCTCATTGCT AGAGAAGTCG CCAACGAGGA AAGAGGAATG
    TTCCTGATCA GTGCATCCTC TGCTGGTCCT GAGATGTACG AAATTCACAC CAACTCCAAG
    GAGGAACGAA ATAACTGGAT GAGACGAATC CAACAGGCCG TGGAAAGTTG CCCAGAAGAA
    GAAGGGGGAA GGACAAGTGA ATCTGATGAA GAGAGGCGGA AAGCTGAAGC AAGAGTGGCC
    AAAATCCAGC AATGTCAAGA AATACTCAGT AACCAAGACC AACAGATTTG CGCATATTTG
    GAGGAGAAGC TGCATATCTA CGCTGAACTT GGAGAACTGA GTGGATTCAA GGATGTCCAT
    CTAGAGCCCC ACCTCCTCAT CAAACCTGAC CCAGGGGAGC CTCCCCAGGC AGCCTCATTA
    CTAGCAGCAG CCCTGAGAGA AGCGGAGAGC CTGCAGGTTG CCGTGAAGGC CTCACAAGCG
    GGTGATGCTC GCCCATCACC TGAGGAGGGT TCTGCAGAAC CTGCGGTTGC AGATGCACAG
    GGTCCTCCCG AGGAGCCAGG ATCATCTCCA GCCTCACTAG TCACAGAAGG GACAGGAGGA
    AGAGGCTGTT GGGATGTGGA TCCTGGCCCC CAGGGTGTGG GAACCGACTT GGCGGTCTCT
    GATGCAGGGG AGAAGGTGGA ATATAGAAGT TTCACGGTTT CTTCACAGTC AGAGATTATA
    CAAGCGATCC AGAATTTAAC CCGACTCCTC TATAGCCTTC AGGCCGCGCT GACCATCCAG
    GACAGCCACA TCCAGGTGCA CAGGCTGCTG CTGCAGCAGG CTGGCCTGCC GGCGTCCCAA
    GAGGAGCACG GCGAGCTGGC GAGGCAGCGG GAGGAGCTGG CCGCCCTGCC CGGGCTCCAG
    TGGCAGCTGC AGCAGGAGCG GCGGCGCGGC CAGCGCACCT GGGAGCGGCA GCAGCGCGAG
    CAGGAGGCCA GGGCCTGCCG GCTGCGGGAG CGCGAGCAGG GCTGCCGCGG CCGCGAGGAG
    CTGCTGCTCA GGGCCCGCGG GGAGCTGGAC CGGCAGCTGC GGGAGCACCG GCAGGACCTG
    GAGCGGCTGC GGGAGGGCCA GCGCCTGGTG GAGAGTGCGA GGGCCCGCAC GCGCGCCCAG
    CACAGCCTGC TGCGTGGCCC GAGGCCCGGG CGCCAGAGCA GCCTGCCCGC GGCCTTGCCT
    CCGGGCAGCG GCGAGGTGAT GGAACTTAAT CGATCCGAGA GCTTGTGTCA TGAAAATTCA
    TTCTTCATTA ATGAAGCTTT AGTACAAATG TCACTTAACA CTTACAACAA ATCAACTCCA
    TCAGGTGTCC ACCAGGAAGC CGCTTATCCC CAGAATATAG CTAATTCGGA CTTGGTAAGG
    ACTAGTGAAA GTCCCGTAGA CCTCCAGTTG GACATTTCTC AGCCCTTGGA TGTCAACCAC
    GAACTGTGGA CAGCCGCTGG GTCCTGTCAT CAGATACCCC CTCTCCACGA AAGCAGCAAG
    GATTCTTGTA AAAATGGAAG AATTTACATC AGTCAGCTAT GTTTCCAGAG CCATTCGTCA
    GAAATGTTTT GGCGACCAGA CTTGACCATC TCAGCTTGGG ATAGAGAGCT TCCGAATCTG
    CAGAACTTCC AACCGCTGTT GGTTGCATTA TGTAGATGGT TGCGTTACGT CCGTACATCC
    CCGGGAGATC TTGCCGTCAT CCTCGAGTCC CCTGAGCAGG TTTTTCAGCT AGAAAGAAAA
    TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe286782
    Clone ID Related Accession (Same CDS sequence) XM_022410517.1
    Accession Version XM_022410517.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4374bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rho guanine nucleotide exchange factor 28 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTTTCTGT GCAGCTCCCC GGCTGGGGCC CCCCAGCTGG GGCCAGGAGG TAAAGCTGCC 
    TGCTCCTCGT CCGGCCCTCG GACTCGGACT CGGCATCACT CTTTCGATGT CTTCAAAAAA
    CCTAAGCATC CCCCAGCGTT CTTTGCTGCA GGCGGGCTCT CTGATGTGCT GAATGGCGGC
    GATGAAGTTT ACGCCAACTG TATGGTGATT GACCAGGTTG GTGATTTGGA TATTAACTAC
    ATCAATTTAG AAGGAATCTC TACAGACGCC TGTGCTGAAT CCATGGGTTC TACTCTGGGG
    CCTCAGAACT GCGAGCATAT CCTTGCTGCT GAAACCAGCC ATGGGAAATA CCCACTAAGT
    GAGAACAAGG AAGTGGTCTC AGATACCGAA GCTCAGCAGT CCTTCCCATC ACCGTCTAGT
    TCATATGCTT CCAATTTTGA TCTTTCCTTT GGTCTTCATG GATTTGAGAA GGAACAAAGT
    CTCCTGAAGA AAAGAAGCTC CAGCCTGGAT GCCTTGGATG CCGACAGCGA AGGGGAAGGG
    CATTCTGAGC GGTCACACAT CTGCTACACG CCAGCATCTC AGAGTTCCTC GAGGACTGGG
    ATTCCCAGTG GGGATGAGCT GGACTCCTTC GAGACCGCCA CCAAACCTGA TTTTAGTATC
    TCCAGGACAG AGTCGCTTTC TTTATCAAGT AGCCTGCAGT TGAAGGAATC ATTGCTTCCT
    GGAGTCCGCT CACGTTCTTA TTCCTGCTCA TCACCCAAAA TTTCTTTAGG AAAAACTCGA
    TTGGTGCGTG ATTTCACAGT GTGCAATTCT GGTGAAGAGC AAAGAGCCTT CGGCTTCCCG
    GAGCCCGCCA GGGAGAGGAG GATTCAAGAG GAAGAATGGG ATAAATACAT CATACCTGCC
    AAGTCAGAGT CTGAGAAATA CAAAGTGAGC CGGACCTTCA GCTTCCTCAT GAACAGGATG
    ACAAGCCCTC GGAACAAATC TAAGACGAAA AGCAAGGATG CCAAAGACAA AGAGAAACCG
    AGCCGACATC AGTTTGTCCC CGGAACCTTC TCTGGGGTTC TCCAGTGTTC CGTTTGTGAT
    AAAACGCTCC TGGGGAAGGA GTCATCCCAG TGTTCCAACT GTAATGCAAA CGTCCACAAG
    GGTTGTAAGG ACGCTGCACC TCCGTGTACC AAGAAATTCC AGGAGAAATA TAACAAGAAC
    AAACCGCAGA CCATCCTTGG AAACTCTTCA TTTCGAGACA TCCCGCAACC CAGTCTCTCC
    TTGCACCCTT CTTCCTCCAT GCCTATCGGA TTGCCCACTG GGAGAAAAGA AGCTTCTGCC
    CAGGCCCATC CCTTGTCCAG AAGTGTTCCA GGCACTGCCT TGGAAAGCTT CAGAAGATCG
    GGGCTATCCC TGGAGTCTGA GAGTGACGGG AACAGCTGGA GAAGCAGGTC TCATTCCGAT
    GAGCTGTTAC AGTCCATGGG CTCTTCTCCC TCCACGGAAT CCTTCTTAAT GGAAGATGTC
    GTGGACTCTT CTCTATGGAG TGACCTCAGC AGTGACGCCC AGGAGTTGGA AGCAGAATCC
    TGGAGTCTGG TGGTGGACCC CTCCTTTTGC AATAAGCAGG AGAAAGATGT CATCAAAAGA
    CAGGATGTCA TTTTCGAGCT TATGCAAACA GAGATGCATC ATATCCAGAC CCTGCTCATC
    ATGTCTGAGA TCTTCAGGAA AGGAATGAAG GAGGAGCTGC AGCTTGATCA CAGCACCGTG
    GATAAAATCT TTCCCTGCTT AGATGAGTTA CTTGAAATCC ACAGGCATTT CTTTTATAGT
    ATGAAGGAGC GAAGGCAGGA GTCCTGTGCT GGTGGTGACA GGAATTTTGT CATCAGCCGA
    ATTGGAGATA TTCTAGTACA GCAGTTTTCA GAAGAAAATG CAAATCAAAT GAAGAAAATA
    TATGGAGAAT TCTGTAGCCA TCACAAAGAA GCTGTTAGCC TCTATAAAGA ACTCCAACAG
    AATAAGAAGT TTCAGAATTT TATTAAGCTT CGAAATAGTA ATCTTTTGGC TCGACGCCGA
    GGAATCCCAG AATGCATTCT GCTAGTCACT CAGCGCATCA CAAAATACCC TGTCCTGGTG
    GAACGGATTT TGCAGTACAC AAAGGAAAGA AGTGAAGAAC ATCAAGACTT GTGCAAAGCT
    CTGTGCTTAA TTAAAGACAT GATTGCAGCA GTGGATTTAA AAGTCAGCGA GTATGAGAAA
    AACCAAAAAT GGCTTGAGAT CCTAAATAAG ATCGAAAACA AAACCTACAC AAAGCTCAAA
    AATGGCCACG TGTTTAGGAA GCAAGCACTG ATAAGTAAAG AAAGGACTCT CTTATATGAT
    GGCCTTGTTT ATTGGAAAAC TGCTACGGGT CGTTTCAAAG ATATCCTGGC TCTACTTCTA
    ACTGATGTGC TGCTCTTTTT ACAAGAAAAA GACCAGAAAT ACATCTTTGC AGCTGTTGAT
    CAGAAGCCAT CGGTTATTTC CCTTCAGAAG CTCATTGCTA GAGAAGTCGC CAACGAGGAA
    AGAGGAATGT TCCTGATCAG TGCATCCTCT GCTGGTCCTG AGATGTACGA AATTCACACC
    AACTCCAAGG AGGAACGAAA TAACTGGATG AGACGAATCC AACAGGCCGT GGAAAGTTGC
    CCAGAAGAAG AAGGGGGAAG GACAAGTGAA TCTGATGAAG AGAGGCGGAA AGCTGAAGCA
    AGAGTGGCCA AAATCCAGCA ATGTCAAGAA ATACTCAGTA ACCAAGACCA ACAGATTTGC
    GCATATTTGG AGGAGAAGCT GCATATCTAC GCTGAACTTG GAGAACTGAG TGGATTCAAG
    GATGTCCATC TAGAGCCCCA CCTCCTCATC AAACCTGACC CAGGGGAGCC TCCCCAGGCA
    GCCTCATTAC TAGCAGCAGC CCTGAGAGAA GCGGAGAGCC TGCAGGTTGC CGTGAAGGCC
    TCACAAGCGG GTGATGCTCG CCCATCACCT GAGGAGGGTT CTGCAGAACC TGCGGTTGCA
    GATGCACAGG GTCCTCCCGA GGAGCCAGGA TCATCTCCAG CCTCACTAGT CACAGAAGGG
    ACAGGAGGAA GAGGCTGTTG GGATGTGGAT CCTGGCCCCC AGGGTGTGGG AACCGACTTG
    GCGGTCTCTG ATGCAGGGGA GAAGAGTCTT AAAAATCTGT GCTGCAGCCG GCAAAGTCCC
    TGGGGTGTCA GTCACCATCA CCAGCTGCAG CCCTGCACAG GGACCACAAG GGGGAGGGCC
    AGCTCCACGA AAAGTAGGAC ACTACACAGC TCAGGCTTGT ATCAGGCTTC GTGGTTCTTG
    GGAGTGGAAT ATAGAAGTTT CACGGTTTCT TCACAGTCAG AGATTATACA AGCGATCCAG
    AATTTAACCC GACTCCTCTA TAGCCTTCAG GCCGCGCTGA CCATCCAGGA CAGCCACATC
    CAGGTGCACA GGCTGCTGCT GCAGCAGGCT GGCCTGCCGG CGTCCCAAGA GGAGCACGGC
    GAGCTGGCGA GGCAGCGGGA GGAGCTGGCC GCCCTGCCCG GGCTCCAGTG GCAGCTGCAG
    CAGGAGCGGC GGCGCGGCCA GCGCACCTGG GAGCGGCAGC AGCGCGAGCA GGAGGCCAGG
    GCCTGCCGGC TGCGGGAGCG CGAGCAGGGC TGCCGCGGCC GCGAGGAGCT GCTGCTCAGG
    GCCCGCGGGG AGCTGGACCG GCAGCTGCGG GAGCACCGGC AGGACCTGGA GCGGCTGCGG
    GAGGGCCAGC GCCTGGTGGA GAGTGCGAGG GCCCGCACGC GCGCCCAGCA CAGCCTGCTG
    CGTGGCCCGA GGCCCGGGCG CCAGAGCAGC CTGCCCGCGG CCTTGCCTCC GGGCAGCGGC
    GAGGTGATGG AACTTAATCG ATCCGAGAGC TTGTGTCATG AAAATTCATT CTTCATTAAT
    GAAGCTTTAG TACAAATGTC ACTTAACACT TACAACAAAT CAACTCCATC AGGTGTCCAC
    CAGGAAGCCG CTTATCCCCA GAATATAGCT AATTCGGACT TGGTAAGGAC TAGTGAAAGT
    CCCGTAGACC TCCAGTTGGA CATTTCTCAG CCCTTGGATG TCAACCACGA ACTGTGGACA
    GCCGCTGGGT CCTGTCATCA GATACCCCCT CTCCACGAAA GCAGCAAGGA TTCTTGTAAA
    AATGGAAGAA TTTACATCAG TCAGCTATGT TTCCAGAGCC ATTCGTCAGA AATGTTTTGG
    CGACCAGACT TGACCATCTC AGCTTGGGAT AGAGAGCTTC CGAATCTGCA GAACTTCCAA
    CCGCTGTTGG TTGCATTATG TAGATGGTTG CGTTACGTCC GTACATCCCC GGGAGATCTT
    GCCGTCATCC TCGAGTCCCC TGAGCAGGTT TTTCAGCTAG AAAGAAAATG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022266225.1
    CDS16..4389
    Translation

    Target ORF information:

    RefSeq Version XM_022410517.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022410517.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTTTCTGT GCAGCTCCCC GGCTGGGGCC CCCCAGCTGG GGCCAGGAGG TAAAGCTGCC 
    TGCTCCTCGT CCGGCCCTCG GACTCGGACT CGGCATCACT CTTTCGATGT CTTCAAAAAA
    CCTAAGCATC CCCCAGCGTT CTTTGCTGCA GGCGGGCTCT CTGATGTGCT GAATGGCGGC
    GATGAAGTTT ACGCCAACTG TATGGTGATT GACCAGGTTG GTGATTTGGA TATTAACTAC
    ATCAATTTAG AAGGAATCTC TACAGACGCC TGTGCTGAAT CCATGGGTTC TACTCTGGGG
    CCTCAGAACT GCGAGCATAT CCTTGCTGCT GAAACCAGCC ATGGGAAATA CCCACTAAGT
    GAGAACAAGG AAGTGGTCTC AGATACCGAA GCTCAGCAGT CCTTCCCATC ACCGTCTAGT
    TCATATGCTT CCAATTTTGA TCTTTCCTTT GGTCTTCATG GATTTGAGAA GGAACAAAGT
    CTCCTGAAGA AAAGAAGCTC CAGCCTGGAT GCCTTGGATG CCGACAGCGA AGGGGAAGGG
    CATTCTGAGC GGTCACACAT CTGCTACACG CCAGCATCTC AGAGTTCCTC GAGGACTGGG
    ATTCCCAGTG GGGATGAGCT GGACTCCTTC GAGACCGCCA CCAAACCTGA TTTTAGTATC
    TCCAGGACAG AGTCGCTTTC TTTATCAAGT AGCCTGCAGT TGAAGGAATC ATTGCTTCCT
    GGAGTCCGCT CACGTTCTTA TTCCTGCTCA TCACCCAAAA TTTCTTTAGG AAAAACTCGA
    TTGGTGCGTG ATTTCACAGT GTGCAATTCT GGTGAAGAGC AAAGAGCCTT CGGCTTCCCG
    GAGCCCGCCA GGGAGAGGAG GATTCAAGAG GAAGAATGGG ATAAATACAT CATACCTGCC
    AAGTCAGAGT CTGAGAAATA CAAAGTGAGC CGGACCTTCA GCTTCCTCAT GAACAGGATG
    ACAAGCCCTC GGAACAAATC TAAGACGAAA AGCAAGGATG CCAAAGACAA AGAGAAACCG
    AGCCGACATC AGTTTGTCCC CGGAACCTTC TCTGGGGTTC TCCAGTGTTC CGTTTGTGAT
    AAAACGCTCC TGGGGAAGGA GTCATCCCAG TGTTCCAACT GTAATGCAAA CGTCCACAAG
    GGTTGTAAGG ACGCTGCACC TCCGTGTACC AAGAAATTCC AGGAGAAATA TAACAAGAAC
    AAACCGCAGA CCATCCTTGG AAACTCTTCA TTTCGAGACA TCCCGCAACC CAGTCTCTCC
    TTGCACCCTT CTTCCTCCAT GCCTATCGGA TTGCCCACTG GGAGAAAAGA AGCTTCTGCC
    CAGGCCCATC CCTTGTCCAG AAGTGTTCCA GGCACTGCCT TGGAAAGCTT CAGAAGATCG
    GGGCTATCCC TGGAGTCTGA GAGTGACGGG AACAGCTGGA GAAGCAGGTC TCATTCCGAT
    GAGCTGTTAC AGTCCATGGG CTCTTCTCCC TCCACGGAAT CCTTCTTAAT GGAAGATGTC
    GTGGACTCTT CTCTATGGAG TGACCTCAGC AGTGACGCCC AGGAGTTGGA AGCAGAATCC
    TGGAGTCTGG TGGTGGACCC CTCCTTTTGC AATAAGCAGG AGAAAGATGT CATCAAAAGA
    CAGGATGTCA TTTTCGAGCT TATGCAAACA GAGATGCATC ATATCCAGAC CCTGCTCATC
    ATGTCTGAGA TCTTCAGGAA AGGAATGAAG GAGGAGCTGC AGCTTGATCA CAGCACCGTG
    GATAAAATCT TTCCCTGCTT AGATGAGTTA CTTGAAATCC ACAGGCATTT CTTTTATAGT
    ATGAAGGAGC GAAGGCAGGA GTCCTGTGCT GGTGGTGACA GGAATTTTGT CATCAGCCGA
    ATTGGAGATA TTCTAGTACA GCAGTTTTCA GAAGAAAATG CAAATCAAAT GAAGAAAATA
    TATGGAGAAT TCTGTAGCCA TCACAAAGAA GCTGTTAGCC TCTATAAAGA ACTCCAACAG
    AATAAGAAGT TTCAGAATTT TATTAAGCTT CGAAATAGTA ATCTTTTGGC TCGACGCCGA
    GGAATCCCAG AATGCATTCT GCTAGTCACT CAGCGCATCA CAAAATACCC TGTCCTGGTG
    GAACGGATTT TGCAGTACAC AAAGGAAAGA AGTGAAGAAC ATCAAGACTT GTGCAAAGCT
    CTGTGCTTAA TTAAAGACAT GATTGCAGCA GTGGATTTAA AAGTCAGCGA GTATGAGAAA
    AACCAAAAAT GGCTTGAGAT CCTAAATAAG ATCGAAAACA AAACCTACAC AAAGCTCAAA
    AATGGCCACG TGTTTAGGAA GCAAGCACTG ATAAGTAAAG AAAGGACTCT CTTATATGAT
    GGCCTTGTTT ATTGGAAAAC TGCTACGGGT CGTTTCAAAG ATATCCTGGC TCTACTTCTA
    ACTGATGTGC TGCTCTTTTT ACAAGAAAAA GACCAGAAAT ACATCTTTGC AGCTGTTGAT
    CAGAAGCCAT CGGTTATTTC CCTTCAGAAG CTCATTGCTA GAGAAGTCGC CAACGAGGAA
    AGAGGAATGT TCCTGATCAG TGCATCCTCT GCTGGTCCTG AGATGTACGA AATTCACACC
    AACTCCAAGG AGGAACGAAA TAACTGGATG AGACGAATCC AACAGGCCGT GGAAAGTTGC
    CCAGAAGAAG AAGGGGGAAG GACAAGTGAA TCTGATGAAG AGAGGCGGAA AGCTGAAGCA
    AGAGTGGCCA AAATCCAGCA ATGTCAAGAA ATACTCAGTA ACCAAGACCA ACAGATTTGC
    GCATATTTGG AGGAGAAGCT GCATATCTAC GCTGAACTTG GAGAACTGAG TGGATTCAAG
    GATGTCCATC TAGAGCCCCA CCTCCTCATC AAACCTGACC CAGGGGAGCC TCCCCAGGCA
    GCCTCATTAC TAGCAGCAGC CCTGAGAGAA GCGGAGAGCC TGCAGGTTGC CGTGAAGGCC
    TCACAAGCGG GTGATGCTCG CCCATCACCT GAGGAGGGTT CTGCAGAACC TGCGGTTGCA
    GATGCACAGG GTCCTCCCGA GGAGCCAGGA TCATCTCCAG CCTCACTAGT CACAGAAGGG
    ACAGGAGGAA GAGGCTGTTG GGATGTGGAT CCTGGCCCCC AGGGTGTGGG AACCGACTTG
    GCGGTCTCTG ATGCAGGGGA GAAGAGTCTT AAAAATCTGT GCTGCAGCCG GCAAAGTCCC
    TGGGGTGTCA GTCACCATCA CCAGCTGCAG CCCTGCACAG GGACCACAAG GGGGAGGGCC
    AGCTCCACGA AAAGTAGGAC ACTACACAGC TCAGGCTTGT ATCAGGCTTC GTGGTTCTTG
    GGAGTGGAAT ATAGAAGTTT CACGGTTTCT TCACAGTCAG AGATTATACA AGCGATCCAG
    AATTTAACCC GACTCCTCTA TAGCCTTCAG GCCGCGCTGA CCATCCAGGA CAGCCACATC
    CAGGTGCACA GGCTGCTGCT GCAGCAGGCT GGCCTGCCGG CGTCCCAAGA GGAGCACGGC
    GAGCTGGCGA GGCAGCGGGA GGAGCTGGCC GCCCTGCCCG GGCTCCAGTG GCAGCTGCAG
    CAGGAGCGGC GGCGCGGCCA GCGCACCTGG GAGCGGCAGC AGCGCGAGCA GGAGGCCAGG
    GCCTGCCGGC TGCGGGAGCG CGAGCAGGGC TGCCGCGGCC GCGAGGAGCT GCTGCTCAGG
    GCCCGCGGGG AGCTGGACCG GCAGCTGCGG GAGCACCGGC AGGACCTGGA GCGGCTGCGG
    GAGGGCCAGC GCCTGGTGGA GAGTGCGAGG GCCCGCACGC GCGCCCAGCA CAGCCTGCTG
    CGTGGCCCGA GGCCCGGGCG CCAGAGCAGC CTGCCCGCGG CCTTGCCTCC GGGCAGCGGC
    GAGGTGATGG AACTTAATCG ATCCGAGAGC TTGTGTCATG AAAATTCATT CTTCATTAAT
    GAAGCTTTAG TACAAATGTC ACTTAACACT TACAACAAAT CAACTCCATC AGGTGTCCAC
    CAGGAAGCCG CTTATCCCCA GAATATAGCT AATTCGGACT TGGTAAGGAC TAGTGAAAGT
    CCCGTAGACC TCCAGTTGGA CATTTCTCAG CCCTTGGATG TCAACCACGA ACTGTGGACA
    GCCGCTGGGT CCTGTCATCA GATACCCCCT CTCCACGAAA GCAGCAAGGA TTCTTGTAAA
    AATGGAAGAA TTTACATCAG TCAGCTATGT TTCCAGAGCC ATTCGTCAGA AATGTTTTGG
    CGACCAGACT TGACCATCTC AGCTTGGGAT AGAGAGCTTC CGAATCTGCA GAACTTCCAA
    CCGCTGTTGG TTGCATTATG TAGATGGTTG CGTTACGTCC GTACATCCCC GGGAGATCTT
    GCCGTCATCC TCGAGTCCCC TGAGCAGGTT TTTCAGCTAG AAAGAAAATG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35379
    Clone ID Related Accession (Same CDS sequence) XM_014109055.1 , XM_014109055.2
    Accession Version XM_014109055.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5346bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product rho guanine nucleotide exchange factor 28 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGAGTTGA GCTGCAGCGA AGTACCTCTT TATGGGCAGA TGACCGTCTA TGCAAAGTTT 
    GGGAAAAATG TTTATCTTCC TAAAGATGCC GAGTTTTACT TTATTTATAA TGGATCTCAT
    CAGAGACACG TCGTGATTGC AAAACGCACT GAGGATAATG TTCTCCAGTC CAGCATTCCA
    GGGCATGGGC TTCAGGAGAC GGTGACGGTG TCTGTATGCC TCTGCTCAGA AGGTTACTCC
    CCAGTGACCA TGGGCTCCGG CTCAGTGACC TACGTGGACA ACATGGCTTG CAGGCTGGCT
    CGTTTGCTGG TCACCCAGGC TGATCGCCTC ACAGCCAGTA GTCATCAGAC CTTTCTGACT
    CCATTTGCCC TGACGGTGGG AGCATTGCCT GCTTTGGACG AGGAGCTGGT GCTGGCTCTG
    ACCCATCTGG AGTTGCCTCT TGAGTGGAGT GTCTTGGGAA ATTCTTCACT TGAAGTTTCT
    TTATGCAGAG AGTCTCTTCT ACACCTGGCT ATGAGATGGG GCCTGCCTAA GCTCTCTCAT
    TTCCTCTTGT GCCTCCCTGG GGGAGTTCAG GCTTTGTCTT TACCCAATGA AGAAGGTGCC
    ACACCATTAG ACTTGGCTTT ACGTGCCGGA CACTCCAAGC TGGTTGAAGA CATCACAAAT
    TTCCAGGGCA GACGTTCCCC CGACCTCTCC CGAGTGCACC TCGGCGGACG TGCCTTCCTG
    CAGTATGTTC ACCCGTCGGG GACACTGACG CTGACGCTGA ACCACACAGC TGAGCATTTG
    TTGGAGGCAG ATATTAAACT CTTCAGGAAA TACTTTTTGG ACAGAGCCTT TCTCCTCAAG
    GCTCTTGACG ACCGAGAAGC TGGGCCAGAG GAGAGCCGGG GCATGCCCTC CCGGGCTGCC
    GCCACTGAGG AGGGTACGCG AGCTGCCTCC CCCGCGCCCA GGAACCCAGA AGAGATTAGG
    AATTTGGTGA CCGGCAGGCC ATCACCTGAA AAGGAGGATG TAAAGCGTGT GGACAGCCCG
    GACGTTGACC CCCATGAACA CGACGGCCGG GACAGTCTTG ATTTGGATCA CTCTTTCGAT
    GTCTTCAAAA AACCTAAGCA TCCCCCAGCG TTCTTTGCTG CAGGCGGGCT CTCTGATGTG
    CTGAATGGCG GCGATGAAGT TTACGCCAAC TGTATGGTGA TTGACCAGGT TGGTGATTTG
    GATATTAACT ACATCAATTT AGAAGGAATC TCTACAGACG CCTGTGCTGA ATCCATGGGT
    TCTACTCTGG GGCCTCAGAA CTGCGAGCAT ATCCTTGCTG CTGAAACCAG CCATGGGAAA
    TACCCACTAA GTGAGAACAA GGAAGTGGTC TCAGATACCG AAGCTCAGCA GTCCTTCCCA
    TCACCGTCTA GTTCATATGC TTCCAATTTT GATCTTTCCT TTGGTCTTCA TGGATTTGAG
    AAGGAACAAA GTCTCCTGAA GAAAAGAAGC TCCAGCCTGG ATGCCTTGGA TGCCGACAGC
    GAAGGGGAAG GGCATTCTGA GCGGTCACAC ATCTGCTACA CGCCAGCATC TCAGAGTTCC
    TCGAGGACTG GGATTCCCAG TGGGGATGAG CTGGACTCCT TCGAGACCGC CACCAAACCT
    GATTTTAGTA TCTCCAGGAC AGAGTCGCTT TCTTTATCAA GTAGCCTGCA GTTGAAGGAA
    TCATTGCTTC CTGGAGTCCG CTCACGTTCT TATTCCTGCT CATCACCCAA AATTTCTTTA
    GGAAAAACTC GATTGGTGCG TGATTTCACA GTGTGCAATT CTGGTGAAGA GCAAAGAGCC
    TTCGGCTTCC CGGAGCCCGC CAGGGAGAGG AGGATTCAAG AGGAAGAATG GGATAAATAC
    ATCATACCTG CCAAGTCAGA GTCTGAGAAA TACAAAGTGA GCCGGACCTT CAGCTTCCTC
    ATGAACAGGA TGACAAGCCC TCGGAACAAA TCTAAGACGA AAAGCAAGGA TGCCAAAGAC
    AAAGAGAAAC CGAGCCGACA TCAGTTTGTC CCCGGAACCT TCTCTGGGGT TCTCCAGTGT
    TCCGTTTGTG ATAAAACGCT CCTGGGGAAG GAGTCATCCC AGTGTTCCAA CTGTAATGCA
    AACGTCCACA AGGGTTGTAA GGACGCTGCA CCTCCGTGTA CCAAGAAATT CCAGGAGAAA
    TATAACAAGA ACAAACCGCA GACCATCCTT GGAAACTCTT CATTTCGAGA CATCCCGCAA
    CCCAGTCTCT CCTTGCACCC TTCTTCCTCC ATGCCTATCG GATTGCCCAC TGGGAGAAAA
    GAAGCTTCTG CCCAGGCCCA TCCCTTGTCC AGAAGTGTTC CAGGCACTGC CTTGGAAAGC
    TTCAGAAGAT CGGGGCTATC CCTGGAGTCT GAGAGTGACG GGAACAGCTG GAGAAGCAGG
    TCTCATTCCG ATGAGCTGTT ACAGTCCATG GGCTCTTCTC CCTCCACGGA ATCCTTCTTA
    ATGGAAGATG TCGTGGACTC TTCTCTATGG AGTGACCTCA GCAGTGACGC CCAGGAGTTG
    GAAGCAGAAT CCTGGAGTCT GGTGGTGGAC CCCTCCTTTT GCAATAAGCA GGAGAAAGAT
    GTCATCAAAA GACAGGATGT CATTTTCGAG CTTATGCAAA CAGAGATGCA TCATATCCAG
    ACCCTGCTCA TCATGTCTGA GATCTTCAGG AAAGGAATGA AGGAGGAGCT GCAGCTTGAT
    CACAGCACCG TGGATAAAAT CTTTCCCTGC TTAGATGAGT TACTTGAAAT CCACAGGCAT
    TTCTTTTATA GTATGAAGGA GCGAAGGCAG GAGTCCTGTG CTGGTGGTGA CAGGAATTTT
    GTCATCAGCC GAATTGGAGA TATTCTAGTA CAGCAGTTTT CAGAAGAAAA TGCAAATCAA
    ATGAAGAAAA TATATGGAGA ATTCTGTAGC CATCACAAAG AAGCTGTTAG CCTCTATAAA
    GAACTCCAAC AGAATAAGAA GTTTCAGAAT TTTATTAAGC TTCGAAATAG TAATCTTTTG
    GCTCGACGCC GAGGAATCCC AGAATGCATT CTGCTAGTCA CTCAGCGCAT CACAAAATAC
    CCTGTCCTGG TGGAACGGAT TTTGCAGTAC ACAAAGGAAA GAAGTGAAGA ACATCAAGAC
    TTGTGCAAAG CTCTGTGCTT AATTAAAGAC ATGATTGCAG CAGTGGATTT AAAAGTCAGC
    GAGTATGAGA AAAACCAAAA ATGGCTTGAG ATCCTAAATA AGATCGAAAA CAAAACCTAC
    ACAAAGCTCA AAAATGGCCA CGTGTTTAGG AAGCAAGCAC TGATAAGTAA AGAAAGGACT
    CTCTTATATG ATGGCCTTGT TTATTGGAAA ACTGCTACGG GTCGTTTCAA AGATATCCTG
    GCTCTACTTC TAACTGATGT GCTGCTCTTT TTACAAGAAA AAGACCAGAA ATACATCTTT
    GCAGCTGTTG ATCAGAAGCC ATCGGTTATT TCCCTTCAGA AGCTCATTGC TAGAGAAGTC
    GCCAACGAGG AAAGAGGAAT GTTCCTGATC AGTGCATCCT CTGCTGGTCC TGAGATGTAC
    GAAATTCACA CCAACTCCAA GGAGGAACGA AATAACTGGA TGAGACGAAT CCAACAGGCC
    GTGGAAAGTT GCCCAGAAGA AGAAGGGGGA AGGACAAGTG AATCTGATGA AGAGAGGCGG
    AAAGCTGAAG CAAGAGTGGC CAAAATCCAG CAATGTCAAG AAATACTCAG TAACCAAGAC
    CAACAGATTT GCGCATATTT GGAGGAGAAG CTGCATATCT ACGCTGAACT TGGAGAACTG
    AGTGGATTCA AGGATGTCCA TCTAGAGCCC CACCTCCTCA TCAAACCTGA CCCAGGGGAG
    CCTCCCCAGG CAGCCTCATT ACTAGCAGCA GCCCTGAGAG AAGCGGAGAG CCTGCAGGTT
    GCCGTGAAGG CCTCACAAGC GGGTGATGCT CGCCCATCAC CTGAGGAGGG TTCTGCAGAA
    CCTGCGGTTG CAGATGCACA GGGTCCTCCC GAGGAGCCAG GATCATCTCC AGCCTCACTA
    GTCACAGAAG GGACAGGAGG AAGAGGCTGT TGGGATGTGG ATCCTGGCCC CCAGGGTGTG
    GGAACCGACT TGGCGGTCTC TGATGCAGGG GAGAAGAGTC TTAAAAATCT GTGCTGCAGC
    CGGCAAAGTC CCTGGGGTGT CAGTCACCAT CACCAGCTGC AGCCCTGCAC AGGGACCACA
    AGGGGGAGGG CCAGCTCCAC GAAAAGTAGG ACACTACACA GCTCAGGCTT GTATCAGGCT
    TCGTGGTTCT TGGGAGTGGA ATATAGAAGT TTCACGGTTT CTTCACAGTC AGAGATTATA
    CAAGCGATCC AGAATTTAAC CCGACTCCTC TATAGCCTTC AGGCCGCGCT GACCATCCAG
    GACAGCCACA TCCAGGTGCA CAGGCTGCTG CTGCAGCAGG CTGGCCTGCC GGCGTCCCAA
    GAGGAGCACG GCGAGCTGGC GAGGCAGCGG GAGGAGCTGG CCGCCCTGCC CGGGCTCCAG
    TGGCAGCTGC AGCAGGAGCG GCGGCGCGGC CAGCGCACCT GGGAGCGGCA GCAGCGCGAG
    CAGGAGGCCA GGGCCTGCCG GCTGCGGGAG CGCGAGCAGG GCTGCCGCGG CCGCGAGGAG
    CTGCTGCTCA GGGCCCGCGG GGAGCTGGAC CGGCAGCTGC GGGAGCACCG GCAGGACCTG
    GAGCGGCTGC GGGAGGGCCA GCGCCTGGTG GAGAGTGCGA GGGCCCGCAC GCGCGCCCAG
    CACAGCCTGC TGCGTGGCCC GAGGCCCGGG CGCCAGAGCA GCCTGCCCGC GGCCTTGCCT
    CCGGGCAGCG GCGAGGTGAT GGAACTTAAT CGATCCGAGA GCTTGTGTCA TGAAAATTCA
    TTCTTCATTA ATGAAGCTTT AGTACAAATG TCACTTAACA CTTACAACAA ATCAACTCCA
    TCAGGTGTCC ACCAGGAAGC CGCTTATCCC CAGAATATAG CTAATTCGGA CTTGGTAAGG
    ACTAGTGAAA GTCCCGTAGA CCTCCAGTTG GACATTTCTC AGCCCTTGGA TGTCAACCAC
    GAACTGTGGA CAGCCGCTGG GTCCTGTCAT CAGATACCCC CTCTCCACGA AAGCAGCAAG
    GATTCTTGTA AAAATGGAAG AATTTACATC AGTCAGCTAT GTTTCCAGAG CCATTCGTCA
    GAAATGTTTT GGCGACCAGA CTTGACCATC TCAGCTTGGG ATAGAGAGCT TCCGAATCTG
    CAGAACTTCC AACCGCTGTT GGTTGCATTA TGTAGATGGT TGCGTTACGT CCGTACATCC
    CCGGGAGATC TTGCCGTCAT CCTCGAGTCC CCTGAGCAGG TTTTTCAGCT AGAAAGAAAA
    TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013964530.1
    CDS12..5357
    Translation

    Target ORF information:

    RefSeq Version XM_014109055.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 28 (ARHGEF28), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109055.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGAGTTGA GCTGCAGCGA AGTACCTCTT TATGGGCAGA TGACCGTCTA TGCAAAGTTT 
    GGGAAAAATG TTTATCTTCC TAAAGATGCC GAGTTTTACT TTATTTATAA TGGATCTCAT
    CAGAGACACG TCGTGATTGC AAAACGCACT GAGGATAATG TTCTCCAGTC CAGCATTCCA
    GGGCATGGGC TTCAGGAGAC GGTGACGGTG TCTGTATGCC TCTGCTCAGA AGGTTACTCC
    CCAGTGACCA TGGGCTCCGG CTCAGTGACC TACGTGGACA ACATGGCTTG CAGGCTGGCT
    CGTTTGCTGG TCACCCAGGC TGATCGCCTC ACAGCCAGTA GTCATCAGAC CTTTCTGACT
    CCATTTGCCC TGACGGTGGG AGCATTGCCT GCTTTGGACG AGGAGCTGGT GCTGGCTCTG
    ACCCATCTGG AGTTGCCTCT TGAGTGGAGT GTCTTGGGAA ATTCTTCACT TGAAGTTTCT
    TTATGCAGAG AGTCTCTTCT ACACCTGGCT ATGAGATGGG GCCTGCCTAA GCTCTCTCAT
    TTCCTCTTGT GCCTCCCTGG GGGAGTTCAG GCTTTGTCTT TACCCAATGA AGAAGGTGCC
    ACACCATTAG ACTTGGCTTT ACGTGCCGGA CACTCCAAGC TGGTTGAAGA CATCACAAAT
    TTCCAGGGCA GACGTTCCCC CGACCTCTCC CGAGTGCACC TCGGCGGACG TGCCTTCCTG
    CAGTATGTTC ACCCGTCGGG GACACTGACG CTGACGCTGA ACCACACAGC TGAGCATTTG
    TTGGAGGCAG ATATTAAACT CTTCAGGAAA TACTTTTTGG ACAGAGCCTT TCTCCTCAAG
    GCTCTTGACG ACCGAGAAGC TGGGCCAGAG GAGAGCCGGG GCATGCCCTC CCGGGCTGCC
    GCCACTGAGG AGGGTACGCG AGCTGCCTCC CCCGCGCCCA GGAACCCAGA AGAGATTAGG
    AATTTGGTGA CCGGCAGGCC ATCACCTGAA AAGGAGGATG TAAAGCGTGT GGACAGCCCG
    GACGTTGACC CCCATGAACA CGACGGCCGG GACAGTCTTG ATTTGGATCA CTCTTTCGAT
    GTCTTCAAAA AACCTAAGCA TCCCCCAGCG TTCTTTGCTG CAGGCGGGCT CTCTGATGTG
    CTGAATGGCG GCGATGAAGT TTACGCCAAC TGTATGGTGA TTGACCAGGT TGGTGATTTG
    GATATTAACT ACATCAATTT AGAAGGAATC TCTACAGACG CCTGTGCTGA ATCCATGGGT
    TCTACTCTGG GGCCTCAGAA CTGCGAGCAT ATCCTTGCTG CTGAAACCAG CCATGGGAAA
    TACCCACTAA GTGAGAACAA GGAAGTGGTC TCAGATACCG AAGCTCAGCA GTCCTTCCCA
    TCACCGTCTA GTTCATATGC TTCCAATTTT GATCTTTCCT TTGGTCTTCA TGGATTTGAG
    AAGGAACAAA GTCTCCTGAA GAAAAGAAGC TCCAGCCTGG ATGCCTTGGA TGCCGACAGC
    GAAGGGGAAG GGCATTCTGA GCGGTCACAC ATCTGCTACA CGCCAGCATC TCAGAGTTCC
    TCGAGGACTG GGATTCCCAG TGGGGATGAG CTGGACTCCT TCGAGACCGC CACCAAACCT
    GATTTTAGTA TCTCCAGGAC AGAGTCGCTT TCTTTATCAA GTAGCCTGCA GTTGAAGGAA
    TCATTGCTTC CTGGAGTCCG CTCACGTTCT TATTCCTGCT CATCACCCAA AATTTCTTTA
    GGAAAAACTC GATTGGTGCG TGATTTCACA GTGTGCAATT CTGGTGAAGA GCAAAGAGCC
    TTCGGCTTCC CGGAGCCCGC CAGGGAGAGG AGGATTCAAG AGGAAGAATG GGATAAATAC
    ATCATACCTG CCAAGTCAGA GTCTGAGAAA TACAAAGTGA GCCGGACCTT CAGCTTCCTC
    ATGAACAGGA TGACAAGCCC TCGGAACAAA TCTAAGACGA AAAGCAAGGA TGCCAAAGAC
    AAAGAGAAAC CGAGCCGACA TCAGTTTGTC CCCGGAACCT TCTCTGGGGT TCTCCAGTGT
    TCCGTTTGTG ATAAAACGCT CCTGGGGAAG GAGTCATCCC AGTGTTCCAA CTGTAATGCA
    AACGTCCACA AGGGTTGTAA GGACGCTGCA CCTCCGTGTA CCAAGAAATT CCAGGAGAAA
    TATAACAAGA ACAAACCGCA GACCATCCTT GGAAACTCTT CATTTCGAGA CATCCCGCAA
    CCCAGTCTCT CCTTGCACCC TTCTTCCTCC ATGCCTATCG GATTGCCCAC TGGGAGAAAA
    GAAGCTTCTG CCCAGGCCCA TCCCTTGTCC AGAAGTGTTC CAGGCACTGC CTTGGAAAGC
    TTCAGAAGAT CGGGGCTATC CCTGGAGTCT GAGAGTGACG GGAACAGCTG GAGAAGCAGG
    TCTCATTCCG ATGAGCTGTT ACAGTCCATG GGCTCTTCTC CCTCCACGGA ATCCTTCTTA
    ATGGAAGATG TCGTGGACTC TTCTCTATGG AGTGACCTCA GCAGTGACGC CCAGGAGTTG
    GAAGCAGAAT CCTGGAGTCT GGTGGTGGAC CCCTCCTTTT GCAATAAGCA GGAGAAAGAT
    GTCATCAAAA GACAGGATGT CATTTTCGAG CTTATGCAAA CAGAGATGCA TCATATCCAG
    ACCCTGCTCA TCATGTCTGA GATCTTCAGG AAAGGAATGA AGGAGGAGCT GCAGCTTGAT
    CACAGCACCG TGGATAAAAT CTTTCCCTGC TTAGATGAGT TACTTGAAAT CCACAGGCAT
    TTCTTTTATA GTATGAAGGA GCGAAGGCAG GAGTCCTGTG CTGGTGGTGA CAGGAATTTT
    GTCATCAGCC GAATTGGAGA TATTCTAGTA CAGCAGTTTT CAGAAGAAAA TGCAAATCAA
    ATGAAGAAAA TATATGGAGA ATTCTGTAGC CATCACAAAG AAGCTGTTAG CCTCTATAAA
    GAACTCCAAC AGAATAAGAA GTTTCAGAAT TTTATTAAGC TTCGAAATAG TAATCTTTTG
    GCTCGACGCC GAGGAATCCC AGAATGCATT CTGCTAGTCA CTCAGCGCAT CACAAAATAC
    CCTGTCCTGG TGGAACGGAT TTTGCAGTAC ACAAAGGAAA GAAGTGAAGA ACATCAAGAC
    TTGTGCAAAG CTCTGTGCTT AATTAAAGAC ATGATTGCAG CAGTGGATTT AAAAGTCAGC
    GAGTATGAGA AAAACCAAAA ATGGCTTGAG ATCCTAAATA AGATCGAAAA CAAAACCTAC
    ACAAAGCTCA AAAATGGCCA CGTGTTTAGG AAGCAAGCAC TGATAAGTAA AGAAAGGACT
    CTCTTATATG ATGGCCTTGT TTATTGGAAA ACTGCTACGG GTCGTTTCAA AGATATCCTG
    GCTCTACTTC TAACTGATGT GCTGCTCTTT TTACAAGAAA AAGACCAGAA ATACATCTTT
    GCAGCTGTTG ATCAGAAGCC ATCGGTTATT TCCCTTCAGA AGCTCATTGC TAGAGAAGTC
    GCCAACGAGG AAAGAGGAAT GTTCCTGATC AGTGCATCCT CTGCTGGTCC TGAGATGTAC
    GAAATTCACA CCAACTCCAA GGAGGAACGA AATAACTGGA TGAGACGAAT CCAACAGGCC
    GTGGAAAGTT GCCCAGAAGA AGAAGGGGGA AGGACAAGTG AATCTGATGA AGAGAGGCGG
    AAAGCTGAAG CAAGAGTGGC CAAAATCCAG CAATGTCAAG AAATACTCAG TAACCAAGAC
    CAACAGATTT GCGCATATTT GGAGGAGAAG CTGCATATCT ACGCTGAACT TGGAGAACTG
    AGTGGATTCA AGGATGTCCA TCTAGAGCCC CACCTCCTCA TCAAACCTGA CCCAGGGGAG
    CCTCCCCAGG CAGCCTCATT ACTAGCAGCA GCCCTGAGAG AAGCGGAGAG CCTGCAGGTT
    GCCGTGAAGG CCTCACAAGC GGGTGATGCT CGCCCATCAC CTGAGGAGGG TTCTGCAGAA
    CCTGCGGTTG CAGATGCACA GGGTCCTCCC GAGGAGCCAG GATCATCTCC AGCCTCACTA
    GTCACAGAAG GGACAGGAGG AAGAGGCTGT TGGGATGTGG ATCCTGGCCC CCAGGGTGTG
    GGAACCGACT TGGCGGTCTC TGATGCAGGG GAGAAGAGTC TTAAAAATCT GTGCTGCAGC
    CGGCAAAGTC CCTGGGGTGT CAGTCACCAT CACCAGCTGC AGCCCTGCAC AGGGACCACA
    AGGGGGAGGG CCAGCTCCAC GAAAAGTAGG ACACTACACA GCTCAGGCTT GTATCAGGCT
    TCGTGGTTCT TGGGAGTGGA ATATAGAAGT TTCACGGTTT CTTCACAGTC AGAGATTATA
    CAAGCGATCC AGAATTTAAC CCGACTCCTC TATAGCCTTC AGGCCGCGCT GACCATCCAG
    GACAGCCACA TCCAGGTGCA CAGGCTGCTG CTGCAGCAGG CTGGCCTGCC GGCGTCCCAA
    GAGGAGCACG GCGAGCTGGC GAGGCAGCGG GAGGAGCTGG CCGCCCTGCC CGGGCTCCAG
    TGGCAGCTGC AGCAGGAGCG GCGGCGCGGC CAGCGCACCT GGGAGCGGCA GCAGCGCGAG
    CAGGAGGCCA GGGCCTGCCG GCTGCGGGAG CGCGAGCAGG GCTGCCGCGG CCGCGAGGAG
    CTGCTGCTCA GGGCCCGCGG GGAGCTGGAC CGGCAGCTGC GGGAGCACCG GCAGGACCTG
    GAGCGGCTGC GGGAGGGCCA GCGCCTGGTG GAGAGTGCGA GGGCCCGCAC GCGCGCCCAG
    CACAGCCTGC TGCGTGGCCC GAGGCCCGGG CGCCAGAGCA GCCTGCCCGC GGCCTTGCCT
    CCGGGCAGCG GCGAGGTGAT GGAACTTAAT CGATCCGAGA GCTTGTGTCA TGAAAATTCA
    TTCTTCATTA ATGAAGCTTT AGTACAAATG TCACTTAACA CTTACAACAA ATCAACTCCA
    TCAGGTGTCC ACCAGGAAGC CGCTTATCCC CAGAATATAG CTAATTCGGA CTTGGTAAGG
    ACTAGTGAAA GTCCCGTAGA CCTCCAGTTG GACATTTCTC AGCCCTTGGA TGTCAACCAC
    GAACTGTGGA CAGCCGCTGG GTCCTGTCAT CAGATACCCC CTCTCCACGA AAGCAGCAAG
    GATTCTTGTA AAAATGGAAG AATTTACATC AGTCAGCTAT GTTTCCAGAG CCATTCGTCA
    GAAATGTTTT GGCGACCAGA CTTGACCATC TCAGCTTGGG ATAGAGAGCT TCCGAATCTG
    CAGAACTTCC AACCGCTGTT GGTTGCATTA TGTAGATGGT TGCGTTACGT CCGTACATCC
    CCGGGAGATC TTGCCGTCAT CCTCGAGTCC CCTGAGCAGG TTTTTCAGCT AGAAAGAAAA
    TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35379
    Clone ID Related Accession (Same CDS sequence) XM_014109055.1 , XM_014109055.2
    Accession Version XM_014109055.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5346bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rho guanine nucleotide exchange factor 28 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109055.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGAGTTGA GCTGCAGCGA AGTACCTCTT TATGGGCAGA TGACCGTCTA TGCAAAGTTT 
    GGGAAAAATG TTTATCTTCC TAAAGATGCC GAGTTTTACT TTATTTATAA TGGATCTCAT
    CAGAGACACG TCGTGATTGC AAAACGCACT GAGGATAATG TTCTCCAGTC CAGCATTCCA
    GGGCATGGGC TTCAGGAGAC GGTGACGGTG TCTGTATGCC TCTGCTCAGA AGGTTACTCC
    CCAGTGACCA TGGGCTCCGG CTCAGTGACC TACGTGGACA ACATGGCTTG CAGGCTGGCT
    CGTTTGCTGG TCACCCAGGC TGATCGCCTC ACAGCCAGTA GTCATCAGAC CTTTCTGACT
    CCATTTGCCC TGACGGTGGG AGCATTGCCT GCTTTGGACG AGGAGCTGGT GCTGGCTCTG
    ACCCATCTGG AGTTGCCTCT TGAGTGGAGT GTCTTGGGAA ATTCTTCACT TGAAGTTTCT
    TTATGCAGAG AGTCTCTTCT ACACCTGGCT ATGAGATGGG GCCTGCCTAA GCTCTCTCAT
    TTCCTCTTGT GCCTCCCTGG GGGAGTTCAG GCTTTGTCTT TACCCAATGA AGAAGGTGCC
    ACACCATTAG ACTTGGCTTT ACGTGCCGGA CACTCCAAGC TGGTTGAAGA CATCACAAAT
    TTCCAGGGCA GACGTTCCCC CGACCTCTCC CGAGTGCACC TCGGCGGACG TGCCTTCCTG
    CAGTATGTTC ACCCGTCGGG GACACTGACG CTGACGCTGA ACCACACAGC TGAGCATTTG
    TTGGAGGCAG ATATTAAACT CTTCAGGAAA TACTTTTTGG ACAGAGCCTT TCTCCTCAAG
    GCTCTTGACG ACCGAGAAGC TGGGCCAGAG GAGAGCCGGG GCATGCCCTC CCGGGCTGCC
    GCCACTGAGG AGGGTACGCG AGCTGCCTCC CCCGCGCCCA GGAACCCAGA AGAGATTAGG
    AATTTGGTGA CCGGCAGGCC ATCACCTGAA AAGGAGGATG TAAAGCGTGT GGACAGCCCG
    GACGTTGACC CCCATGAACA CGACGGCCGG GACAGTCTTG ATTTGGATCA CTCTTTCGAT
    GTCTTCAAAA AACCTAAGCA TCCCCCAGCG TTCTTTGCTG CAGGCGGGCT CTCTGATGTG
    CTGAATGGCG GCGATGAAGT TTACGCCAAC TGTATGGTGA TTGACCAGGT TGGTGATTTG
    GATATTAACT ACATCAATTT AGAAGGAATC TCTACAGACG CCTGTGCTGA ATCCATGGGT
    TCTACTCTGG GGCCTCAGAA CTGCGAGCAT ATCCTTGCTG CTGAAACCAG CCATGGGAAA
    TACCCACTAA GTGAGAACAA GGAAGTGGTC TCAGATACCG AAGCTCAGCA GTCCTTCCCA
    TCACCGTCTA GTTCATATGC TTCCAATTTT GATCTTTCCT TTGGTCTTCA TGGATTTGAG
    AAGGAACAAA GTCTCCTGAA GAAAAGAAGC TCCAGCCTGG ATGCCTTGGA TGCCGACAGC
    GAAGGGGAAG GGCATTCTGA GCGGTCACAC ATCTGCTACA CGCCAGCATC TCAGAGTTCC
    TCGAGGACTG GGATTCCCAG TGGGGATGAG CTGGACTCCT TCGAGACCGC CACCAAACCT
    GATTTTAGTA TCTCCAGGAC AGAGTCGCTT TCTTTATCAA GTAGCCTGCA GTTGAAGGAA
    TCATTGCTTC CTGGAGTCCG CTCACGTTCT TATTCCTGCT CATCACCCAA AATTTCTTTA
    GGAAAAACTC GATTGGTGCG TGATTTCACA GTGTGCAATT CTGGTGAAGA GCAAAGAGCC
    TTCGGCTTCC CGGAGCCCGC CAGGGAGAGG AGGATTCAAG AGGAAGAATG GGATAAATAC
    ATCATACCTG CCAAGTCAGA GTCTGAGAAA TACAAAGTGA GCCGGACCTT CAGCTTCCTC
    ATGAACAGGA TGACAAGCCC TCGGAACAAA TCTAAGACGA AAAGCAAGGA TGCCAAAGAC
    AAAGAGAAAC CGAGCCGACA TCAGTTTGTC CCCGGAACCT TCTCTGGGGT TCTCCAGTGT
    TCCGTTTGTG ATAAAACGCT CCTGGGGAAG GAGTCATCCC AGTGTTCCAA CTGTAATGCA
    AACGTCCACA AGGGTTGTAA GGACGCTGCA CCTCCGTGTA CCAAGAAATT CCAGGAGAAA
    TATAACAAGA ACAAACCGCA GACCATCCTT GGAAACTCTT CATTTCGAGA CATCCCGCAA
    CCCAGTCTCT CCTTGCACCC TTCTTCCTCC ATGCCTATCG GATTGCCCAC TGGGAGAAAA
    GAAGCTTCTG CCCAGGCCCA TCCCTTGTCC AGAAGTGTTC CAGGCACTGC CTTGGAAAGC
    TTCAGAAGAT CGGGGCTATC CCTGGAGTCT GAGAGTGACG GGAACAGCTG GAGAAGCAGG
    TCTCATTCCG ATGAGCTGTT ACAGTCCATG GGCTCTTCTC CCTCCACGGA ATCCTTCTTA
    ATGGAAGATG TCGTGGACTC TTCTCTATGG AGTGACCTCA GCAGTGACGC CCAGGAGTTG
    GAAGCAGAAT CCTGGAGTCT GGTGGTGGAC CCCTCCTTTT GCAATAAGCA GGAGAAAGAT
    GTCATCAAAA GACAGGATGT CATTTTCGAG CTTATGCAAA CAGAGATGCA TCATATCCAG
    ACCCTGCTCA TCATGTCTGA GATCTTCAGG AAAGGAATGA AGGAGGAGCT GCAGCTTGAT
    CACAGCACCG TGGATAAAAT CTTTCCCTGC TTAGATGAGT TACTTGAAAT CCACAGGCAT
    TTCTTTTATA GTATGAAGGA GCGAAGGCAG GAGTCCTGTG CTGGTGGTGA CAGGAATTTT
    GTCATCAGCC GAATTGGAGA TATTCTAGTA CAGCAGTTTT CAGAAGAAAA TGCAAATCAA
    ATGAAGAAAA TATATGGAGA ATTCTGTAGC CATCACAAAG AAGCTGTTAG CCTCTATAAA
    GAACTCCAAC AGAATAAGAA GTTTCAGAAT TTTATTAAGC TTCGAAATAG TAATCTTTTG
    GCTCGACGCC GAGGAATCCC AGAATGCATT CTGCTAGTCA CTCAGCGCAT CACAAAATAC
    CCTGTCCTGG TGGAACGGAT TTTGCAGTAC ACAAAGGAAA GAAGTGAAGA ACATCAAGAC
    TTGTGCAAAG CTCTGTGCTT AATTAAAGAC ATGATTGCAG CAGTGGATTT AAAAGTCAGC
    GAGTATGAGA AAAACCAAAA ATGGCTTGAG ATCCTAAATA AGATCGAAAA CAAAACCTAC
    ACAAAGCTCA AAAATGGCCA CGTGTTTAGG AAGCAAGCAC TGATAAGTAA AGAAAGGACT
    CTCTTATATG ATGGCCTTGT TTATTGGAAA ACTGCTACGG GTCGTTTCAA AGATATCCTG
    GCTCTACTTC TAACTGATGT GCTGCTCTTT TTACAAGAAA AAGACCAGAA ATACATCTTT
    GCAGCTGTTG ATCAGAAGCC ATCGGTTATT TCCCTTCAGA AGCTCATTGC TAGAGAAGTC
    GCCAACGAGG AAAGAGGAAT GTTCCTGATC AGTGCATCCT CTGCTGGTCC TGAGATGTAC
    GAAATTCACA CCAACTCCAA GGAGGAACGA AATAACTGGA TGAGACGAAT CCAACAGGCC
    GTGGAAAGTT GCCCAGAAGA AGAAGGGGGA AGGACAAGTG AATCTGATGA AGAGAGGCGG
    AAAGCTGAAG CAAGAGTGGC CAAAATCCAG CAATGTCAAG AAATACTCAG TAACCAAGAC
    CAACAGATTT GCGCATATTT GGAGGAGAAG CTGCATATCT ACGCTGAACT TGGAGAACTG
    AGTGGATTCA AGGATGTCCA TCTAGAGCCC CACCTCCTCA TCAAACCTGA CCCAGGGGAG
    CCTCCCCAGG CAGCCTCATT ACTAGCAGCA GCCCTGAGAG AAGCGGAGAG CCTGCAGGTT
    GCCGTGAAGG CCTCACAAGC GGGTGATGCT CGCCCATCAC CTGAGGAGGG TTCTGCAGAA
    CCTGCGGTTG CAGATGCACA GGGTCCTCCC GAGGAGCCAG GATCATCTCC AGCCTCACTA
    GTCACAGAAG GGACAGGAGG AAGAGGCTGT TGGGATGTGG ATCCTGGCCC CCAGGGTGTG
    GGAACCGACT TGGCGGTCTC TGATGCAGGG GAGAAGAGTC TTAAAAATCT GTGCTGCAGC
    CGGCAAAGTC CCTGGGGTGT CAGTCACCAT CACCAGCTGC AGCCCTGCAC AGGGACCACA
    AGGGGGAGGG CCAGCTCCAC GAAAAGTAGG ACACTACACA GCTCAGGCTT GTATCAGGCT
    TCGTGGTTCT TGGGAGTGGA ATATAGAAGT TTCACGGTTT CTTCACAGTC AGAGATTATA
    CAAGCGATCC AGAATTTAAC CCGACTCCTC TATAGCCTTC AGGCCGCGCT GACCATCCAG
    GACAGCCACA TCCAGGTGCA CAGGCTGCTG CTGCAGCAGG CTGGCCTGCC GGCGTCCCAA
    GAGGAGCACG GCGAGCTGGC GAGGCAGCGG GAGGAGCTGG CCGCCCTGCC CGGGCTCCAG
    TGGCAGCTGC AGCAGGAGCG GCGGCGCGGC CAGCGCACCT GGGAGCGGCA GCAGCGCGAG
    CAGGAGGCCA GGGCCTGCCG GCTGCGGGAG CGCGAGCAGG GCTGCCGCGG CCGCGAGGAG
    CTGCTGCTCA GGGCCCGCGG GGAGCTGGAC CGGCAGCTGC GGGAGCACCG GCAGGACCTG
    GAGCGGCTGC GGGAGGGCCA GCGCCTGGTG GAGAGTGCGA GGGCCCGCAC GCGCGCCCAG
    CACAGCCTGC TGCGTGGCCC GAGGCCCGGG CGCCAGAGCA GCCTGCCCGC GGCCTTGCCT
    CCGGGCAGCG GCGAGGTGAT GGAACTTAAT CGATCCGAGA GCTTGTGTCA TGAAAATTCA
    TTCTTCATTA ATGAAGCTTT AGTACAAATG TCACTTAACA CTTACAACAA ATCAACTCCA
    TCAGGTGTCC ACCAGGAAGC CGCTTATCCC CAGAATATAG CTAATTCGGA CTTGGTAAGG
    ACTAGTGAAA GTCCCGTAGA CCTCCAGTTG GACATTTCTC AGCCCTTGGA TGTCAACCAC
    GAACTGTGGA CAGCCGCTGG GTCCTGTCAT CAGATACCCC CTCTCCACGA AAGCAGCAAG
    GATTCTTGTA AAAATGGAAG AATTTACATC AGTCAGCTAT GTTTCCAGAG CCATTCGTCA
    GAAATGTTTT GGCGACCAGA CTTGACCATC TCAGCTTGGG ATAGAGAGCT TCCGAATCTG
    CAGAACTTCC AACCGCTGTT GGTTGCATTA TGTAGATGGT TGCGTTACGT CCGTACATCC
    CCGGGAGATC TTGCCGTCAT CCTCGAGTCC CCTGAGCAGG TTTTTCAGCT AGAAAGAAAA
    TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013964530.1
    CDS87..5432
    Translation

    Target ORF information:

    RefSeq Version XM_014109055.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109055.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGAGTTGA GCTGCAGCGA AGTACCTCTT TATGGGCAGA TGACCGTCTA TGCAAAGTTT 
    GGGAAAAATG TTTATCTTCC TAAAGATGCC GAGTTTTACT TTATTTATAA TGGATCTCAT
    CAGAGACACG TCGTGATTGC AAAACGCACT GAGGATAATG TTCTCCAGTC CAGCATTCCA
    GGGCATGGGC TTCAGGAGAC GGTGACGGTG TCTGTATGCC TCTGCTCAGA AGGTTACTCC
    CCAGTGACCA TGGGCTCCGG CTCAGTGACC TACGTGGACA ACATGGCTTG CAGGCTGGCT
    CGTTTGCTGG TCACCCAGGC TGATCGCCTC ACAGCCAGTA GTCATCAGAC CTTTCTGACT
    CCATTTGCCC TGACGGTGGG AGCATTGCCT GCTTTGGACG AGGAGCTGGT GCTGGCTCTG
    ACCCATCTGG AGTTGCCTCT TGAGTGGAGT GTCTTGGGAA ATTCTTCACT TGAAGTTTCT
    TTATGCAGAG AGTCTCTTCT ACACCTGGCT ATGAGATGGG GCCTGCCTAA GCTCTCTCAT
    TTCCTCTTGT GCCTCCCTGG GGGAGTTCAG GCTTTGTCTT TACCCAATGA AGAAGGTGCC
    ACACCATTAG ACTTGGCTTT ACGTGCCGGA CACTCCAAGC TGGTTGAAGA CATCACAAAT
    TTCCAGGGCA GACGTTCCCC CGACCTCTCC CGAGTGCACC TCGGCGGACG TGCCTTCCTG
    CAGTATGTTC ACCCGTCGGG GACACTGACG CTGACGCTGA ACCACACAGC TGAGCATTTG
    TTGGAGGCAG ATATTAAACT CTTCAGGAAA TACTTTTTGG ACAGAGCCTT TCTCCTCAAG
    GCTCTTGACG ACCGAGAAGC TGGGCCAGAG GAGAGCCGGG GCATGCCCTC CCGGGCTGCC
    GCCACTGAGG AGGGTACGCG AGCTGCCTCC CCCGCGCCCA GGAACCCAGA AGAGATTAGG
    AATTTGGTGA CCGGCAGGCC ATCACCTGAA AAGGAGGATG TAAAGCGTGT GGACAGCCCG
    GACGTTGACC CCCATGAACA CGACGGCCGG GACAGTCTTG ATTTGGATCA CTCTTTCGAT
    GTCTTCAAAA AACCTAAGCA TCCCCCAGCG TTCTTTGCTG CAGGCGGGCT CTCTGATGTG
    CTGAATGGCG GCGATGAAGT TTACGCCAAC TGTATGGTGA TTGACCAGGT TGGTGATTTG
    GATATTAACT ACATCAATTT AGAAGGAATC TCTACAGACG CCTGTGCTGA ATCCATGGGT
    TCTACTCTGG GGCCTCAGAA CTGCGAGCAT ATCCTTGCTG CTGAAACCAG CCATGGGAAA
    TACCCACTAA GTGAGAACAA GGAAGTGGTC TCAGATACCG AAGCTCAGCA GTCCTTCCCA
    TCACCGTCTA GTTCATATGC TTCCAATTTT GATCTTTCCT TTGGTCTTCA TGGATTTGAG
    AAGGAACAAA GTCTCCTGAA GAAAAGAAGC TCCAGCCTGG ATGCCTTGGA TGCCGACAGC
    GAAGGGGAAG GGCATTCTGA GCGGTCACAC ATCTGCTACA CGCCAGCATC TCAGAGTTCC
    TCGAGGACTG GGATTCCCAG TGGGGATGAG CTGGACTCCT TCGAGACCGC CACCAAACCT
    GATTTTAGTA TCTCCAGGAC AGAGTCGCTT TCTTTATCAA GTAGCCTGCA GTTGAAGGAA
    TCATTGCTTC CTGGAGTCCG CTCACGTTCT TATTCCTGCT CATCACCCAA AATTTCTTTA
    GGAAAAACTC GATTGGTGCG TGATTTCACA GTGTGCAATT CTGGTGAAGA GCAAAGAGCC
    TTCGGCTTCC CGGAGCCCGC CAGGGAGAGG AGGATTCAAG AGGAAGAATG GGATAAATAC
    ATCATACCTG CCAAGTCAGA GTCTGAGAAA TACAAAGTGA GCCGGACCTT CAGCTTCCTC
    ATGAACAGGA TGACAAGCCC TCGGAACAAA TCTAAGACGA AAAGCAAGGA TGCCAAAGAC
    AAAGAGAAAC CGAGCCGACA TCAGTTTGTC CCCGGAACCT TCTCTGGGGT TCTCCAGTGT
    TCCGTTTGTG ATAAAACGCT CCTGGGGAAG GAGTCATCCC AGTGTTCCAA CTGTAATGCA
    AACGTCCACA AGGGTTGTAA GGACGCTGCA CCTCCGTGTA CCAAGAAATT CCAGGAGAAA
    TATAACAAGA ACAAACCGCA GACCATCCTT GGAAACTCTT CATTTCGAGA CATCCCGCAA
    CCCAGTCTCT CCTTGCACCC TTCTTCCTCC ATGCCTATCG GATTGCCCAC TGGGAGAAAA
    GAAGCTTCTG CCCAGGCCCA TCCCTTGTCC AGAAGTGTTC CAGGCACTGC CTTGGAAAGC
    TTCAGAAGAT CGGGGCTATC CCTGGAGTCT GAGAGTGACG GGAACAGCTG GAGAAGCAGG
    TCTCATTCCG ATGAGCTGTT ACAGTCCATG GGCTCTTCTC CCTCCACGGA ATCCTTCTTA
    ATGGAAGATG TCGTGGACTC TTCTCTATGG AGTGACCTCA GCAGTGACGC CCAGGAGTTG
    GAAGCAGAAT CCTGGAGTCT GGTGGTGGAC CCCTCCTTTT GCAATAAGCA GGAGAAAGAT
    GTCATCAAAA GACAGGATGT CATTTTCGAG CTTATGCAAA CAGAGATGCA TCATATCCAG
    ACCCTGCTCA TCATGTCTGA GATCTTCAGG AAAGGAATGA AGGAGGAGCT GCAGCTTGAT
    CACAGCACCG TGGATAAAAT CTTTCCCTGC TTAGATGAGT TACTTGAAAT CCACAGGCAT
    TTCTTTTATA GTATGAAGGA GCGAAGGCAG GAGTCCTGTG CTGGTGGTGA CAGGAATTTT
    GTCATCAGCC GAATTGGAGA TATTCTAGTA CAGCAGTTTT CAGAAGAAAA TGCAAATCAA
    ATGAAGAAAA TATATGGAGA ATTCTGTAGC CATCACAAAG AAGCTGTTAG CCTCTATAAA
    GAACTCCAAC AGAATAAGAA GTTTCAGAAT TTTATTAAGC TTCGAAATAG TAATCTTTTG
    GCTCGACGCC GAGGAATCCC AGAATGCATT CTGCTAGTCA CTCAGCGCAT CACAAAATAC
    CCTGTCCTGG TGGAACGGAT TTTGCAGTAC ACAAAGGAAA GAAGTGAAGA ACATCAAGAC
    TTGTGCAAAG CTCTGTGCTT AATTAAAGAC ATGATTGCAG CAGTGGATTT AAAAGTCAGC
    GAGTATGAGA AAAACCAAAA ATGGCTTGAG ATCCTAAATA AGATCGAAAA CAAAACCTAC
    ACAAAGCTCA AAAATGGCCA CGTGTTTAGG AAGCAAGCAC TGATAAGTAA AGAAAGGACT
    CTCTTATATG ATGGCCTTGT TTATTGGAAA ACTGCTACGG GTCGTTTCAA AGATATCCTG
    GCTCTACTTC TAACTGATGT GCTGCTCTTT TTACAAGAAA AAGACCAGAA ATACATCTTT
    GCAGCTGTTG ATCAGAAGCC ATCGGTTATT TCCCTTCAGA AGCTCATTGC TAGAGAAGTC
    GCCAACGAGG AAAGAGGAAT GTTCCTGATC AGTGCATCCT CTGCTGGTCC TGAGATGTAC
    GAAATTCACA CCAACTCCAA GGAGGAACGA AATAACTGGA TGAGACGAAT CCAACAGGCC
    GTGGAAAGTT GCCCAGAAGA AGAAGGGGGA AGGACAAGTG AATCTGATGA AGAGAGGCGG
    AAAGCTGAAG CAAGAGTGGC CAAAATCCAG CAATGTCAAG AAATACTCAG TAACCAAGAC
    CAACAGATTT GCGCATATTT GGAGGAGAAG CTGCATATCT ACGCTGAACT TGGAGAACTG
    AGTGGATTCA AGGATGTCCA TCTAGAGCCC CACCTCCTCA TCAAACCTGA CCCAGGGGAG
    CCTCCCCAGG CAGCCTCATT ACTAGCAGCA GCCCTGAGAG AAGCGGAGAG CCTGCAGGTT
    GCCGTGAAGG CCTCACAAGC GGGTGATGCT CGCCCATCAC CTGAGGAGGG TTCTGCAGAA
    CCTGCGGTTG CAGATGCACA GGGTCCTCCC GAGGAGCCAG GATCATCTCC AGCCTCACTA
    GTCACAGAAG GGACAGGAGG AAGAGGCTGT TGGGATGTGG ATCCTGGCCC CCAGGGTGTG
    GGAACCGACT TGGCGGTCTC TGATGCAGGG GAGAAGAGTC TTAAAAATCT GTGCTGCAGC
    CGGCAAAGTC CCTGGGGTGT CAGTCACCAT CACCAGCTGC AGCCCTGCAC AGGGACCACA
    AGGGGGAGGG CCAGCTCCAC GAAAAGTAGG ACACTACACA GCTCAGGCTT GTATCAGGCT
    TCGTGGTTCT TGGGAGTGGA ATATAGAAGT TTCACGGTTT CTTCACAGTC AGAGATTATA
    CAAGCGATCC AGAATTTAAC CCGACTCCTC TATAGCCTTC AGGCCGCGCT GACCATCCAG
    GACAGCCACA TCCAGGTGCA CAGGCTGCTG CTGCAGCAGG CTGGCCTGCC GGCGTCCCAA
    GAGGAGCACG GCGAGCTGGC GAGGCAGCGG GAGGAGCTGG CCGCCCTGCC CGGGCTCCAG
    TGGCAGCTGC AGCAGGAGCG GCGGCGCGGC CAGCGCACCT GGGAGCGGCA GCAGCGCGAG
    CAGGAGGCCA GGGCCTGCCG GCTGCGGGAG CGCGAGCAGG GCTGCCGCGG CCGCGAGGAG
    CTGCTGCTCA GGGCCCGCGG GGAGCTGGAC CGGCAGCTGC GGGAGCACCG GCAGGACCTG
    GAGCGGCTGC GGGAGGGCCA GCGCCTGGTG GAGAGTGCGA GGGCCCGCAC GCGCGCCCAG
    CACAGCCTGC TGCGTGGCCC GAGGCCCGGG CGCCAGAGCA GCCTGCCCGC GGCCTTGCCT
    CCGGGCAGCG GCGAGGTGAT GGAACTTAAT CGATCCGAGA GCTTGTGTCA TGAAAATTCA
    TTCTTCATTA ATGAAGCTTT AGTACAAATG TCACTTAACA CTTACAACAA ATCAACTCCA
    TCAGGTGTCC ACCAGGAAGC CGCTTATCCC CAGAATATAG CTAATTCGGA CTTGGTAAGG
    ACTAGTGAAA GTCCCGTAGA CCTCCAGTTG GACATTTCTC AGCCCTTGGA TGTCAACCAC
    GAACTGTGGA CAGCCGCTGG GTCCTGTCAT CAGATACCCC CTCTCCACGA AAGCAGCAAG
    GATTCTTGTA AAAATGGAAG AATTTACATC AGTCAGCTAT GTTTCCAGAG CCATTCGTCA
    GAAATGTTTT GGCGACCAGA CTTGACCATC TCAGCTTGGG ATAGAGAGCT TCCGAATCTG
    CAGAACTTCC AACCGCTGTT GGTTGCATTA TGTAGATGGT TGCGTTACGT CCGTACATCC
    CCGGGAGATC TTGCCGTCAT CCTCGAGTCC CCTGAGCAGG TTTTTCAGCT AGAAAGAAAA
    TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35380
    Clone ID Related Accession (Same CDS sequence) XM_014109057.2 , XM_014109057.1
    Accession Version XM_014109057.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5307bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rho guanine nucleotide exchange factor 28 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109057.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGAGTTGA GCTGCAGCGA AGTACCTCTT TATGGGCAGA TGACCGTCTA TGCAAAGTTT 
    GGGAAAAATG TTTATCTTCC TAAAGATGCC GAGTTTTACT TTATTTATAA TGGATCTCAT
    CAGAGACACG TCGTGATTGC AAAACGCACT GAGGATAATG TTCTCCAGTC CAGCATTCCA
    GGGCATGGGC TTCAGGAGAC GGTGACGGTG TCTGTATGCC TCTGCTCAGA AGGTTACTCC
    CCAGTGACCA TGGGCTCCGG CTCAGTGACC TACGTGGACA ACATGGCTTG CAGGCTGGCT
    CGTTTGCTGG TCACCCAGGC TGATCGCCTC ACAGCCAGTA GTCATCAGAC CTTTCTGACT
    CCATTTGCCC TGACGGTGGG AGCATTGCCT GCTTTGGACG AGGAGCTGGT GCTGGCTCTG
    ACCCATCTGG AGTTGCCTCT TGAGTGGAGT GTCTTGGGAA ATTCTTCACT TGAAGTTTCT
    TTATGCAGAG AGTCTCTTCT ACACCTGGCT ATGAGATGGG GCCTGCCTAA GCTCTCTCAT
    TTCCTCTTGT GCCTCCCTGG GGGAGTTCAG GCTTTGTCTT TACCCAATGA AGAAGGTGCC
    ACACCATTAG ACTTGGCTTT ACGTGCCGGA CACTCCAAGC TGGTTGAAGA CATCACAAAT
    TTCCAGGGCA GACGTTCCCC CGACCTCTCC CGAGTGCACC TCGGCGGACG TGCCTTCCTG
    CAGTATGTTC ACCCGTCGGG GACACTGACG CTGACGCTGA ACCACACAGC TGAGCATTTG
    TTGGAGGCAG ATATTAAACT CTTCAGGAAA TACTTTTTGG ACAGAGCCTT TCTCCTCAAG
    GCTCTTGACG ACCGAGAAGC TGGGCCAGAG GAGAGCCGGG GCATGCCCTC CCGGGCTGCC
    GCCACTGAGG AGGAGATTAG GAATTTGGTG ACCGGCAGGC CATCACCTGA AAAGGAGGAT
    GTAAAGCGTG TGGACAGCCC GGACGTTGAC CCCCATGAAC ACGACGGCCG GGACAGTCTT
    GATTTGGATC ACTCTTTCGA TGTCTTCAAA AAACCTAAGC ATCCCCCAGC GTTCTTTGCT
    GCAGGCGGGC TCTCTGATGT GCTGAATGGC GGCGATGAAG TTTACGCCAA CTGTATGGTG
    ATTGACCAGG TTGGTGATTT GGATATTAAC TACATCAATT TAGAAGGAAT CTCTACAGAC
    GCCTGTGCTG AATCCATGGG TTCTACTCTG GGGCCTCAGA ACTGCGAGCA TATCCTTGCT
    GCTGAAACCA GCCATGGGAA ATACCCACTA AGTGAGAACA AGGAAGTGGT CTCAGATACC
    GAAGCTCAGC AGTCCTTCCC ATCACCGTCT AGTTCATATG CTTCCAATTT TGATCTTTCC
    TTTGGTCTTC ATGGATTTGA GAAGGAACAA AGTCTCCTGA AGAAAAGAAG CTCCAGCCTG
    GATGCCTTGG ATGCCGACAG CGAAGGGGAA GGGCATTCTG AGCGGTCACA CATCTGCTAC
    ACGCCAGCAT CTCAGAGTTC CTCGAGGACT GGGATTCCCA GTGGGGATGA GCTGGACTCC
    TTCGAGACCG CCACCAAACC TGATTTTAGT ATCTCCAGGA CAGAGTCGCT TTCTTTATCA
    AGTAGCCTGC AGTTGAAGGA ATCATTGCTT CCTGGAGTCC GCTCACGTTC TTATTCCTGC
    TCATCACCCA AAATTTCTTT AGGAAAAACT CGATTGGTGC GTGATTTCAC AGTGTGCAAT
    TCTGGTGAAG AGCAAAGAGC CTTCGGCTTC CCGGAGCCCG CCAGGGAGAG GAGGATTCAA
    GAGGAAGAAT GGGATAAATA CATCATACCT GCCAAGTCAG AGTCTGAGAA ATACAAAGTG
    AGCCGGACCT TCAGCTTCCT CATGAACAGG ATGACAAGCC CTCGGAACAA ATCTAAGACG
    AAAAGCAAGG ATGCCAAAGA CAAAGAGAAA CCGAGCCGAC ATCAGTTTGT CCCCGGAACC
    TTCTCTGGGG TTCTCCAGTG TTCCGTTTGT GATAAAACGC TCCTGGGGAA GGAGTCATCC
    CAGTGTTCCA ACTGTAATGC AAACGTCCAC AAGGGTTGTA AGGACGCTGC ACCTCCGTGT
    ACCAAGAAAT TCCAGGAGAA ATATAACAAG AACAAACCGC AGACCATCCT TGGAAACTCT
    TCATTTCGAG ACATCCCGCA ACCCAGTCTC TCCTTGCACC CTTCTTCCTC CATGCCTATC
    GGATTGCCCA CTGGGAGAAA AGAAGCTTCT GCCCAGGCCC ATCCCTTGTC CAGAAGTGTT
    CCAGGCACTG CCTTGGAAAG CTTCAGAAGA TCGGGGCTAT CCCTGGAGTC TGAGAGTGAC
    GGGAACAGCT GGAGAAGCAG GTCTCATTCC GATGAGCTGT TACAGTCCAT GGGCTCTTCT
    CCCTCCACGG AATCCTTCTT AATGGAAGAT GTCGTGGACT CTTCTCTATG GAGTGACCTC
    AGCAGTGACG CCCAGGAGTT GGAAGCAGAA TCCTGGAGTC TGGTGGTGGA CCCCTCCTTT
    TGCAATAAGC AGGAGAAAGA TGTCATCAAA AGACAGGATG TCATTTTCGA GCTTATGCAA
    ACAGAGATGC ATCATATCCA GACCCTGCTC ATCATGTCTG AGATCTTCAG GAAAGGAATG
    AAGGAGGAGC TGCAGCTTGA TCACAGCACC GTGGATAAAA TCTTTCCCTG CTTAGATGAG
    TTACTTGAAA TCCACAGGCA TTTCTTTTAT AGTATGAAGG AGCGAAGGCA GGAGTCCTGT
    GCTGGTGGTG ACAGGAATTT TGTCATCAGC CGAATTGGAG ATATTCTAGT ACAGCAGTTT
    TCAGAAGAAA ATGCAAATCA AATGAAGAAA ATATATGGAG AATTCTGTAG CCATCACAAA
    GAAGCTGTTA GCCTCTATAA AGAACTCCAA CAGAATAAGA AGTTTCAGAA TTTTATTAAG
    CTTCGAAATA GTAATCTTTT GGCTCGACGC CGAGGAATCC CAGAATGCAT TCTGCTAGTC
    ACTCAGCGCA TCACAAAATA CCCTGTCCTG GTGGAACGGA TTTTGCAGTA CACAAAGGAA
    AGAAGTGAAG AACATCAAGA CTTGTGCAAA GCTCTGTGCT TAATTAAAGA CATGATTGCA
    GCAGTGGATT TAAAAGTCAG CGAGTATGAG AAAAACCAAA AATGGCTTGA GATCCTAAAT
    AAGATCGAAA ACAAAACCTA CACAAAGCTC AAAAATGGCC ACGTGTTTAG GAAGCAAGCA
    CTGATAAGTA AAGAAAGGAC TCTCTTATAT GATGGCCTTG TTTATTGGAA AACTGCTACG
    GGTCGTTTCA AAGATATCCT GGCTCTACTT CTAACTGATG TGCTGCTCTT TTTACAAGAA
    AAAGACCAGA AATACATCTT TGCAGCTGTT GATCAGAAGC CATCGGTTAT TTCCCTTCAG
    AAGCTCATTG CTAGAGAAGT CGCCAACGAG GAAAGAGGAA TGTTCCTGAT CAGTGCATCC
    TCTGCTGGTC CTGAGATGTA CGAAATTCAC ACCAACTCCA AGGAGGAACG AAATAACTGG
    ATGAGACGAA TCCAACAGGC CGTGGAAAGT TGCCCAGAAG AAGAAGGGGG AAGGACAAGT
    GAATCTGATG AAGAGAGGCG GAAAGCTGAA GCAAGAGTGG CCAAAATCCA GCAATGTCAA
    GAAATACTCA GTAACCAAGA CCAACAGATT TGCGCATATT TGGAGGAGAA GCTGCATATC
    TACGCTGAAC TTGGAGAACT GAGTGGATTC AAGGATGTCC ATCTAGAGCC CCACCTCCTC
    ATCAAACCTG ACCCAGGGGA GCCTCCCCAG GCAGCCTCAT TACTAGCAGC AGCCCTGAGA
    GAAGCGGAGA GCCTGCAGGT TGCCGTGAAG GCCTCACAAG CGGGTGATGC TCGCCCATCA
    CCTGAGGAGG GTTCTGCAGA ACCTGCGGTT GCAGATGCAC AGGGTCCTCC CGAGGAGCCA
    GGATCATCTC CAGCCTCACT AGTCACAGAA GGGACAGGAG GAAGAGGCTG TTGGGATGTG
    GATCCTGGCC CCCAGGGTGT GGGAACCGAC TTGGCGGTCT CTGATGCAGG GGAGAAGAGT
    CTTAAAAATC TGTGCTGCAG CCGGCAAAGT CCCTGGGGTG TCAGTCACCA TCACCAGCTG
    CAGCCCTGCA CAGGGACCAC AAGGGGGAGG GCCAGCTCCA CGAAAAGTAG GACACTACAC
    AGCTCAGGCT TGTATCAGGC TTCGTGGTTC TTGGGAGTGG AATATAGAAG TTTCACGGTT
    TCTTCACAGT CAGAGATTAT ACAAGCGATC CAGAATTTAA CCCGACTCCT CTATAGCCTT
    CAGGCCGCGC TGACCATCCA GGACAGCCAC ATCCAGGTGC ACAGGCTGCT GCTGCAGCAG
    GCTGGCCTGC CGGCGTCCCA AGAGGAGCAC GGCGAGCTGG CGAGGCAGCG GGAGGAGCTG
    GCCGCCCTGC CCGGGCTCCA GTGGCAGCTG CAGCAGGAGC GGCGGCGCGG CCAGCGCACC
    TGGGAGCGGC AGCAGCGCGA GCAGGAGGCC AGGGCCTGCC GGCTGCGGGA GCGCGAGCAG
    GGCTGCCGCG GCCGCGAGGA GCTGCTGCTC AGGGCCCGCG GGGAGCTGGA CCGGCAGCTG
    CGGGAGCACC GGCAGGACCT GGAGCGGCTG CGGGAGGGCC AGCGCCTGGT GGAGAGTGCG
    AGGGCCCGCA CGCGCGCCCA GCACAGCCTG CTGCGTGGCC CGAGGCCCGG GCGCCAGAGC
    AGCCTGCCCG CGGCCTTGCC TCCGGGCAGC GGCGAGGTGA TGGAACTTAA TCGATCCGAG
    AGCTTGTGTC ATGAAAATTC ATTCTTCATT AATGAAGCTT TAGTACAAAT GTCACTTAAC
    ACTTACAACA AATCAACTCC ATCAGGTGTC CACCAGGAAG CCGCTTATCC CCAGAATATA
    GCTAATTCGG ACTTGGTAAG GACTAGTGAA AGTCCCGTAG ACCTCCAGTT GGACATTTCT
    CAGCCCTTGG ATGTCAACCA CGAACTGTGG ACAGCCGCTG GGTCCTGTCA TCAGATACCC
    CCTCTCCACG AAAGCAGCAA GGATTCTTGT AAAAATGGAA GAATTTACAT CAGTCAGCTA
    TGTTTCCAGA GCCATTCGTC AGAAATGTTT TGGCGACCAG ACTTGACCAT CTCAGCTTGG
    GATAGAGAGC TTCCGAATCT GCAGAACTTC CAACCGCTGT TGGTTGCATT ATGTAGATGG
    TTGCGTTACG TCCGTACATC CCCGGGAGAT CTTGCCGTCA TCCTCGAGTC CCCTGAGCAG
    GTTTTTCAGC TAGAAAGAAA ATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013964532.1
    CDS87..5393
    Translation

    Target ORF information:

    RefSeq Version XM_014109057.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109057.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGAGTTGA GCTGCAGCGA AGTACCTCTT TATGGGCAGA TGACCGTCTA TGCAAAGTTT 
    GGGAAAAATG TTTATCTTCC TAAAGATGCC GAGTTTTACT TTATTTATAA TGGATCTCAT
    CAGAGACACG TCGTGATTGC AAAACGCACT GAGGATAATG TTCTCCAGTC CAGCATTCCA
    GGGCATGGGC TTCAGGAGAC GGTGACGGTG TCTGTATGCC TCTGCTCAGA AGGTTACTCC
    CCAGTGACCA TGGGCTCCGG CTCAGTGACC TACGTGGACA ACATGGCTTG CAGGCTGGCT
    CGTTTGCTGG TCACCCAGGC TGATCGCCTC ACAGCCAGTA GTCATCAGAC CTTTCTGACT
    CCATTTGCCC TGACGGTGGG AGCATTGCCT GCTTTGGACG AGGAGCTGGT GCTGGCTCTG
    ACCCATCTGG AGTTGCCTCT TGAGTGGAGT GTCTTGGGAA ATTCTTCACT TGAAGTTTCT
    TTATGCAGAG AGTCTCTTCT ACACCTGGCT ATGAGATGGG GCCTGCCTAA GCTCTCTCAT
    TTCCTCTTGT GCCTCCCTGG GGGAGTTCAG GCTTTGTCTT TACCCAATGA AGAAGGTGCC
    ACACCATTAG ACTTGGCTTT ACGTGCCGGA CACTCCAAGC TGGTTGAAGA CATCACAAAT
    TTCCAGGGCA GACGTTCCCC CGACCTCTCC CGAGTGCACC TCGGCGGACG TGCCTTCCTG
    CAGTATGTTC ACCCGTCGGG GACACTGACG CTGACGCTGA ACCACACAGC TGAGCATTTG
    TTGGAGGCAG ATATTAAACT CTTCAGGAAA TACTTTTTGG ACAGAGCCTT TCTCCTCAAG
    GCTCTTGACG ACCGAGAAGC TGGGCCAGAG GAGAGCCGGG GCATGCCCTC CCGGGCTGCC
    GCCACTGAGG AGGAGATTAG GAATTTGGTG ACCGGCAGGC CATCACCTGA AAAGGAGGAT
    GTAAAGCGTG TGGACAGCCC GGACGTTGAC CCCCATGAAC ACGACGGCCG GGACAGTCTT
    GATTTGGATC ACTCTTTCGA TGTCTTCAAA AAACCTAAGC ATCCCCCAGC GTTCTTTGCT
    GCAGGCGGGC TCTCTGATGT GCTGAATGGC GGCGATGAAG TTTACGCCAA CTGTATGGTG
    ATTGACCAGG TTGGTGATTT GGATATTAAC TACATCAATT TAGAAGGAAT CTCTACAGAC
    GCCTGTGCTG AATCCATGGG TTCTACTCTG GGGCCTCAGA ACTGCGAGCA TATCCTTGCT
    GCTGAAACCA GCCATGGGAA ATACCCACTA AGTGAGAACA AGGAAGTGGT CTCAGATACC
    GAAGCTCAGC AGTCCTTCCC ATCACCGTCT AGTTCATATG CTTCCAATTT TGATCTTTCC
    TTTGGTCTTC ATGGATTTGA GAAGGAACAA AGTCTCCTGA AGAAAAGAAG CTCCAGCCTG
    GATGCCTTGG ATGCCGACAG CGAAGGGGAA GGGCATTCTG AGCGGTCACA CATCTGCTAC
    ACGCCAGCAT CTCAGAGTTC CTCGAGGACT GGGATTCCCA GTGGGGATGA GCTGGACTCC
    TTCGAGACCG CCACCAAACC TGATTTTAGT ATCTCCAGGA CAGAGTCGCT TTCTTTATCA
    AGTAGCCTGC AGTTGAAGGA ATCATTGCTT CCTGGAGTCC GCTCACGTTC TTATTCCTGC
    TCATCACCCA AAATTTCTTT AGGAAAAACT CGATTGGTGC GTGATTTCAC AGTGTGCAAT
    TCTGGTGAAG AGCAAAGAGC CTTCGGCTTC CCGGAGCCCG CCAGGGAGAG GAGGATTCAA
    GAGGAAGAAT GGGATAAATA CATCATACCT GCCAAGTCAG AGTCTGAGAA ATACAAAGTG
    AGCCGGACCT TCAGCTTCCT CATGAACAGG ATGACAAGCC CTCGGAACAA ATCTAAGACG
    AAAAGCAAGG ATGCCAAAGA CAAAGAGAAA CCGAGCCGAC ATCAGTTTGT CCCCGGAACC
    TTCTCTGGGG TTCTCCAGTG TTCCGTTTGT GATAAAACGC TCCTGGGGAA GGAGTCATCC
    CAGTGTTCCA ACTGTAATGC AAACGTCCAC AAGGGTTGTA AGGACGCTGC ACCTCCGTGT
    ACCAAGAAAT TCCAGGAGAA ATATAACAAG AACAAACCGC AGACCATCCT TGGAAACTCT
    TCATTTCGAG ACATCCCGCA ACCCAGTCTC TCCTTGCACC CTTCTTCCTC CATGCCTATC
    GGATTGCCCA CTGGGAGAAA AGAAGCTTCT GCCCAGGCCC ATCCCTTGTC CAGAAGTGTT
    CCAGGCACTG CCTTGGAAAG CTTCAGAAGA TCGGGGCTAT CCCTGGAGTC TGAGAGTGAC
    GGGAACAGCT GGAGAAGCAG GTCTCATTCC GATGAGCTGT TACAGTCCAT GGGCTCTTCT
    CCCTCCACGG AATCCTTCTT AATGGAAGAT GTCGTGGACT CTTCTCTATG GAGTGACCTC
    AGCAGTGACG CCCAGGAGTT GGAAGCAGAA TCCTGGAGTC TGGTGGTGGA CCCCTCCTTT
    TGCAATAAGC AGGAGAAAGA TGTCATCAAA AGACAGGATG TCATTTTCGA GCTTATGCAA
    ACAGAGATGC ATCATATCCA GACCCTGCTC ATCATGTCTG AGATCTTCAG GAAAGGAATG
    AAGGAGGAGC TGCAGCTTGA TCACAGCACC GTGGATAAAA TCTTTCCCTG CTTAGATGAG
    TTACTTGAAA TCCACAGGCA TTTCTTTTAT AGTATGAAGG AGCGAAGGCA GGAGTCCTGT
    GCTGGTGGTG ACAGGAATTT TGTCATCAGC CGAATTGGAG ATATTCTAGT ACAGCAGTTT
    TCAGAAGAAA ATGCAAATCA AATGAAGAAA ATATATGGAG AATTCTGTAG CCATCACAAA
    GAAGCTGTTA GCCTCTATAA AGAACTCCAA CAGAATAAGA AGTTTCAGAA TTTTATTAAG
    CTTCGAAATA GTAATCTTTT GGCTCGACGC CGAGGAATCC CAGAATGCAT TCTGCTAGTC
    ACTCAGCGCA TCACAAAATA CCCTGTCCTG GTGGAACGGA TTTTGCAGTA CACAAAGGAA
    AGAAGTGAAG AACATCAAGA CTTGTGCAAA GCTCTGTGCT TAATTAAAGA CATGATTGCA
    GCAGTGGATT TAAAAGTCAG CGAGTATGAG AAAAACCAAA AATGGCTTGA GATCCTAAAT
    AAGATCGAAA ACAAAACCTA CACAAAGCTC AAAAATGGCC ACGTGTTTAG GAAGCAAGCA
    CTGATAAGTA AAGAAAGGAC TCTCTTATAT GATGGCCTTG TTTATTGGAA AACTGCTACG
    GGTCGTTTCA AAGATATCCT GGCTCTACTT CTAACTGATG TGCTGCTCTT TTTACAAGAA
    AAAGACCAGA AATACATCTT TGCAGCTGTT GATCAGAAGC CATCGGTTAT TTCCCTTCAG
    AAGCTCATTG CTAGAGAAGT CGCCAACGAG GAAAGAGGAA TGTTCCTGAT CAGTGCATCC
    TCTGCTGGTC CTGAGATGTA CGAAATTCAC ACCAACTCCA AGGAGGAACG AAATAACTGG
    ATGAGACGAA TCCAACAGGC CGTGGAAAGT TGCCCAGAAG AAGAAGGGGG AAGGACAAGT
    GAATCTGATG AAGAGAGGCG GAAAGCTGAA GCAAGAGTGG CCAAAATCCA GCAATGTCAA
    GAAATACTCA GTAACCAAGA CCAACAGATT TGCGCATATT TGGAGGAGAA GCTGCATATC
    TACGCTGAAC TTGGAGAACT GAGTGGATTC AAGGATGTCC ATCTAGAGCC CCACCTCCTC
    ATCAAACCTG ACCCAGGGGA GCCTCCCCAG GCAGCCTCAT TACTAGCAGC AGCCCTGAGA
    GAAGCGGAGA GCCTGCAGGT TGCCGTGAAG GCCTCACAAG CGGGTGATGC TCGCCCATCA
    CCTGAGGAGG GTTCTGCAGA ACCTGCGGTT GCAGATGCAC AGGGTCCTCC CGAGGAGCCA
    GGATCATCTC CAGCCTCACT AGTCACAGAA GGGACAGGAG GAAGAGGCTG TTGGGATGTG
    GATCCTGGCC CCCAGGGTGT GGGAACCGAC TTGGCGGTCT CTGATGCAGG GGAGAAGAGT
    CTTAAAAATC TGTGCTGCAG CCGGCAAAGT CCCTGGGGTG TCAGTCACCA TCACCAGCTG
    CAGCCCTGCA CAGGGACCAC AAGGGGGAGG GCCAGCTCCA CGAAAAGTAG GACACTACAC
    AGCTCAGGCT TGTATCAGGC TTCGTGGTTC TTGGGAGTGG AATATAGAAG TTTCACGGTT
    TCTTCACAGT CAGAGATTAT ACAAGCGATC CAGAATTTAA CCCGACTCCT CTATAGCCTT
    CAGGCCGCGC TGACCATCCA GGACAGCCAC ATCCAGGTGC ACAGGCTGCT GCTGCAGCAG
    GCTGGCCTGC CGGCGTCCCA AGAGGAGCAC GGCGAGCTGG CGAGGCAGCG GGAGGAGCTG
    GCCGCCCTGC CCGGGCTCCA GTGGCAGCTG CAGCAGGAGC GGCGGCGCGG CCAGCGCACC
    TGGGAGCGGC AGCAGCGCGA GCAGGAGGCC AGGGCCTGCC GGCTGCGGGA GCGCGAGCAG
    GGCTGCCGCG GCCGCGAGGA GCTGCTGCTC AGGGCCCGCG GGGAGCTGGA CCGGCAGCTG
    CGGGAGCACC GGCAGGACCT GGAGCGGCTG CGGGAGGGCC AGCGCCTGGT GGAGAGTGCG
    AGGGCCCGCA CGCGCGCCCA GCACAGCCTG CTGCGTGGCC CGAGGCCCGG GCGCCAGAGC
    AGCCTGCCCG CGGCCTTGCC TCCGGGCAGC GGCGAGGTGA TGGAACTTAA TCGATCCGAG
    AGCTTGTGTC ATGAAAATTC ATTCTTCATT AATGAAGCTT TAGTACAAAT GTCACTTAAC
    ACTTACAACA AATCAACTCC ATCAGGTGTC CACCAGGAAG CCGCTTATCC CCAGAATATA
    GCTAATTCGG ACTTGGTAAG GACTAGTGAA AGTCCCGTAG ACCTCCAGTT GGACATTTCT
    CAGCCCTTGG ATGTCAACCA CGAACTGTGG ACAGCCGCTG GGTCCTGTCA TCAGATACCC
    CCTCTCCACG AAAGCAGCAA GGATTCTTGT AAAAATGGAA GAATTTACAT CAGTCAGCTA
    TGTTTCCAGA GCCATTCGTC AGAAATGTTT TGGCGACCAG ACTTGACCAT CTCAGCTTGG
    GATAGAGAGC TTCCGAATCT GCAGAACTTC CAACCGCTGT TGGTTGCATT ATGTAGATGG
    TTGCGTTACG TCCGTACATC CCCGGGAGAT CTTGCCGTCA TCCTCGAGTC CCCTGAGCAG
    GTTTTTCAGC TAGAAAGAAA ATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35380
    Clone ID Related Accession (Same CDS sequence) XM_014109057.2 , XM_014109057.1
    Accession Version XM_014109057.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5307bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product rho guanine nucleotide exchange factor 28 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGAGTTGA GCTGCAGCGA AGTACCTCTT TATGGGCAGA TGACCGTCTA TGCAAAGTTT 
    GGGAAAAATG TTTATCTTCC TAAAGATGCC GAGTTTTACT TTATTTATAA TGGATCTCAT
    CAGAGACACG TCGTGATTGC AAAACGCACT GAGGATAATG TTCTCCAGTC CAGCATTCCA
    GGGCATGGGC TTCAGGAGAC GGTGACGGTG TCTGTATGCC TCTGCTCAGA AGGTTACTCC
    CCAGTGACCA TGGGCTCCGG CTCAGTGACC TACGTGGACA ACATGGCTTG CAGGCTGGCT
    CGTTTGCTGG TCACCCAGGC TGATCGCCTC ACAGCCAGTA GTCATCAGAC CTTTCTGACT
    CCATTTGCCC TGACGGTGGG AGCATTGCCT GCTTTGGACG AGGAGCTGGT GCTGGCTCTG
    ACCCATCTGG AGTTGCCTCT TGAGTGGAGT GTCTTGGGAA ATTCTTCACT TGAAGTTTCT
    TTATGCAGAG AGTCTCTTCT ACACCTGGCT ATGAGATGGG GCCTGCCTAA GCTCTCTCAT
    TTCCTCTTGT GCCTCCCTGG GGGAGTTCAG GCTTTGTCTT TACCCAATGA AGAAGGTGCC
    ACACCATTAG ACTTGGCTTT ACGTGCCGGA CACTCCAAGC TGGTTGAAGA CATCACAAAT
    TTCCAGGGCA GACGTTCCCC CGACCTCTCC CGAGTGCACC TCGGCGGACG TGCCTTCCTG
    CAGTATGTTC ACCCGTCGGG GACACTGACG CTGACGCTGA ACCACACAGC TGAGCATTTG
    TTGGAGGCAG ATATTAAACT CTTCAGGAAA TACTTTTTGG ACAGAGCCTT TCTCCTCAAG
    GCTCTTGACG ACCGAGAAGC TGGGCCAGAG GAGAGCCGGG GCATGCCCTC CCGGGCTGCC
    GCCACTGAGG AGGAGATTAG GAATTTGGTG ACCGGCAGGC CATCACCTGA AAAGGAGGAT
    GTAAAGCGTG TGGACAGCCC GGACGTTGAC CCCCATGAAC ACGACGGCCG GGACAGTCTT
    GATTTGGATC ACTCTTTCGA TGTCTTCAAA AAACCTAAGC ATCCCCCAGC GTTCTTTGCT
    GCAGGCGGGC TCTCTGATGT GCTGAATGGC GGCGATGAAG TTTACGCCAA CTGTATGGTG
    ATTGACCAGG TTGGTGATTT GGATATTAAC TACATCAATT TAGAAGGAAT CTCTACAGAC
    GCCTGTGCTG AATCCATGGG TTCTACTCTG GGGCCTCAGA ACTGCGAGCA TATCCTTGCT
    GCTGAAACCA GCCATGGGAA ATACCCACTA AGTGAGAACA AGGAAGTGGT CTCAGATACC
    GAAGCTCAGC AGTCCTTCCC ATCACCGTCT AGTTCATATG CTTCCAATTT TGATCTTTCC
    TTTGGTCTTC ATGGATTTGA GAAGGAACAA AGTCTCCTGA AGAAAAGAAG CTCCAGCCTG
    GATGCCTTGG ATGCCGACAG CGAAGGGGAA GGGCATTCTG AGCGGTCACA CATCTGCTAC
    ACGCCAGCAT CTCAGAGTTC CTCGAGGACT GGGATTCCCA GTGGGGATGA GCTGGACTCC
    TTCGAGACCG CCACCAAACC TGATTTTAGT ATCTCCAGGA CAGAGTCGCT TTCTTTATCA
    AGTAGCCTGC AGTTGAAGGA ATCATTGCTT CCTGGAGTCC GCTCACGTTC TTATTCCTGC
    TCATCACCCA AAATTTCTTT AGGAAAAACT CGATTGGTGC GTGATTTCAC AGTGTGCAAT
    TCTGGTGAAG AGCAAAGAGC CTTCGGCTTC CCGGAGCCCG CCAGGGAGAG GAGGATTCAA
    GAGGAAGAAT GGGATAAATA CATCATACCT GCCAAGTCAG AGTCTGAGAA ATACAAAGTG
    AGCCGGACCT TCAGCTTCCT CATGAACAGG ATGACAAGCC CTCGGAACAA ATCTAAGACG
    AAAAGCAAGG ATGCCAAAGA CAAAGAGAAA CCGAGCCGAC ATCAGTTTGT CCCCGGAACC
    TTCTCTGGGG TTCTCCAGTG TTCCGTTTGT GATAAAACGC TCCTGGGGAA GGAGTCATCC
    CAGTGTTCCA ACTGTAATGC AAACGTCCAC AAGGGTTGTA AGGACGCTGC ACCTCCGTGT
    ACCAAGAAAT TCCAGGAGAA ATATAACAAG AACAAACCGC AGACCATCCT TGGAAACTCT
    TCATTTCGAG ACATCCCGCA ACCCAGTCTC TCCTTGCACC CTTCTTCCTC CATGCCTATC
    GGATTGCCCA CTGGGAGAAA AGAAGCTTCT GCCCAGGCCC ATCCCTTGTC CAGAAGTGTT
    CCAGGCACTG CCTTGGAAAG CTTCAGAAGA TCGGGGCTAT CCCTGGAGTC TGAGAGTGAC
    GGGAACAGCT GGAGAAGCAG GTCTCATTCC GATGAGCTGT TACAGTCCAT GGGCTCTTCT
    CCCTCCACGG AATCCTTCTT AATGGAAGAT GTCGTGGACT CTTCTCTATG GAGTGACCTC
    AGCAGTGACG CCCAGGAGTT GGAAGCAGAA TCCTGGAGTC TGGTGGTGGA CCCCTCCTTT
    TGCAATAAGC AGGAGAAAGA TGTCATCAAA AGACAGGATG TCATTTTCGA GCTTATGCAA
    ACAGAGATGC ATCATATCCA GACCCTGCTC ATCATGTCTG AGATCTTCAG GAAAGGAATG
    AAGGAGGAGC TGCAGCTTGA TCACAGCACC GTGGATAAAA TCTTTCCCTG CTTAGATGAG
    TTACTTGAAA TCCACAGGCA TTTCTTTTAT AGTATGAAGG AGCGAAGGCA GGAGTCCTGT
    GCTGGTGGTG ACAGGAATTT TGTCATCAGC CGAATTGGAG ATATTCTAGT ACAGCAGTTT
    TCAGAAGAAA ATGCAAATCA AATGAAGAAA ATATATGGAG AATTCTGTAG CCATCACAAA
    GAAGCTGTTA GCCTCTATAA AGAACTCCAA CAGAATAAGA AGTTTCAGAA TTTTATTAAG
    CTTCGAAATA GTAATCTTTT GGCTCGACGC CGAGGAATCC CAGAATGCAT TCTGCTAGTC
    ACTCAGCGCA TCACAAAATA CCCTGTCCTG GTGGAACGGA TTTTGCAGTA CACAAAGGAA
    AGAAGTGAAG AACATCAAGA CTTGTGCAAA GCTCTGTGCT TAATTAAAGA CATGATTGCA
    GCAGTGGATT TAAAAGTCAG CGAGTATGAG AAAAACCAAA AATGGCTTGA GATCCTAAAT
    AAGATCGAAA ACAAAACCTA CACAAAGCTC AAAAATGGCC ACGTGTTTAG GAAGCAAGCA
    CTGATAAGTA AAGAAAGGAC TCTCTTATAT GATGGCCTTG TTTATTGGAA AACTGCTACG
    GGTCGTTTCA AAGATATCCT GGCTCTACTT CTAACTGATG TGCTGCTCTT TTTACAAGAA
    AAAGACCAGA AATACATCTT TGCAGCTGTT GATCAGAAGC CATCGGTTAT TTCCCTTCAG
    AAGCTCATTG CTAGAGAAGT CGCCAACGAG GAAAGAGGAA TGTTCCTGAT CAGTGCATCC
    TCTGCTGGTC CTGAGATGTA CGAAATTCAC ACCAACTCCA AGGAGGAACG AAATAACTGG
    ATGAGACGAA TCCAACAGGC CGTGGAAAGT TGCCCAGAAG AAGAAGGGGG AAGGACAAGT
    GAATCTGATG AAGAGAGGCG GAAAGCTGAA GCAAGAGTGG CCAAAATCCA GCAATGTCAA
    GAAATACTCA GTAACCAAGA CCAACAGATT TGCGCATATT TGGAGGAGAA GCTGCATATC
    TACGCTGAAC TTGGAGAACT GAGTGGATTC AAGGATGTCC ATCTAGAGCC CCACCTCCTC
    ATCAAACCTG ACCCAGGGGA GCCTCCCCAG GCAGCCTCAT TACTAGCAGC AGCCCTGAGA
    GAAGCGGAGA GCCTGCAGGT TGCCGTGAAG GCCTCACAAG CGGGTGATGC TCGCCCATCA
    CCTGAGGAGG GTTCTGCAGA ACCTGCGGTT GCAGATGCAC AGGGTCCTCC CGAGGAGCCA
    GGATCATCTC CAGCCTCACT AGTCACAGAA GGGACAGGAG GAAGAGGCTG TTGGGATGTG
    GATCCTGGCC CCCAGGGTGT GGGAACCGAC TTGGCGGTCT CTGATGCAGG GGAGAAGAGT
    CTTAAAAATC TGTGCTGCAG CCGGCAAAGT CCCTGGGGTG TCAGTCACCA TCACCAGCTG
    CAGCCCTGCA CAGGGACCAC AAGGGGGAGG GCCAGCTCCA CGAAAAGTAG GACACTACAC
    AGCTCAGGCT TGTATCAGGC TTCGTGGTTC TTGGGAGTGG AATATAGAAG TTTCACGGTT
    TCTTCACAGT CAGAGATTAT ACAAGCGATC CAGAATTTAA CCCGACTCCT CTATAGCCTT
    CAGGCCGCGC TGACCATCCA GGACAGCCAC ATCCAGGTGC ACAGGCTGCT GCTGCAGCAG
    GCTGGCCTGC CGGCGTCCCA AGAGGAGCAC GGCGAGCTGG CGAGGCAGCG GGAGGAGCTG
    GCCGCCCTGC CCGGGCTCCA GTGGCAGCTG CAGCAGGAGC GGCGGCGCGG CCAGCGCACC
    TGGGAGCGGC AGCAGCGCGA GCAGGAGGCC AGGGCCTGCC GGCTGCGGGA GCGCGAGCAG
    GGCTGCCGCG GCCGCGAGGA GCTGCTGCTC AGGGCCCGCG GGGAGCTGGA CCGGCAGCTG
    CGGGAGCACC GGCAGGACCT GGAGCGGCTG CGGGAGGGCC AGCGCCTGGT GGAGAGTGCG
    AGGGCCCGCA CGCGCGCCCA GCACAGCCTG CTGCGTGGCC CGAGGCCCGG GCGCCAGAGC
    AGCCTGCCCG CGGCCTTGCC TCCGGGCAGC GGCGAGGTGA TGGAACTTAA TCGATCCGAG
    AGCTTGTGTC ATGAAAATTC ATTCTTCATT AATGAAGCTT TAGTACAAAT GTCACTTAAC
    ACTTACAACA AATCAACTCC ATCAGGTGTC CACCAGGAAG CCGCTTATCC CCAGAATATA
    GCTAATTCGG ACTTGGTAAG GACTAGTGAA AGTCCCGTAG ACCTCCAGTT GGACATTTCT
    CAGCCCTTGG ATGTCAACCA CGAACTGTGG ACAGCCGCTG GGTCCTGTCA TCAGATACCC
    CCTCTCCACG AAAGCAGCAA GGATTCTTGT AAAAATGGAA GAATTTACAT CAGTCAGCTA
    TGTTTCCAGA GCCATTCGTC AGAAATGTTT TGGCGACCAG ACTTGACCAT CTCAGCTTGG
    GATAGAGAGC TTCCGAATCT GCAGAACTTC CAACCGCTGT TGGTTGCATT ATGTAGATGG
    TTGCGTTACG TCCGTACATC CCCGGGAGAT CTTGCCGTCA TCCTCGAGTC CCCTGAGCAG
    GTTTTTCAGC TAGAAAGAAA ATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013964532.1
    CDS12..5318
    Translation

    Target ORF information:

    RefSeq Version XM_014109057.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 28 (ARHGEF28), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109057.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGAGTTGA GCTGCAGCGA AGTACCTCTT TATGGGCAGA TGACCGTCTA TGCAAAGTTT 
    GGGAAAAATG TTTATCTTCC TAAAGATGCC GAGTTTTACT TTATTTATAA TGGATCTCAT
    CAGAGACACG TCGTGATTGC AAAACGCACT GAGGATAATG TTCTCCAGTC CAGCATTCCA
    GGGCATGGGC TTCAGGAGAC GGTGACGGTG TCTGTATGCC TCTGCTCAGA AGGTTACTCC
    CCAGTGACCA TGGGCTCCGG CTCAGTGACC TACGTGGACA ACATGGCTTG CAGGCTGGCT
    CGTTTGCTGG TCACCCAGGC TGATCGCCTC ACAGCCAGTA GTCATCAGAC CTTTCTGACT
    CCATTTGCCC TGACGGTGGG AGCATTGCCT GCTTTGGACG AGGAGCTGGT GCTGGCTCTG
    ACCCATCTGG AGTTGCCTCT TGAGTGGAGT GTCTTGGGAA ATTCTTCACT TGAAGTTTCT
    TTATGCAGAG AGTCTCTTCT ACACCTGGCT ATGAGATGGG GCCTGCCTAA GCTCTCTCAT
    TTCCTCTTGT GCCTCCCTGG GGGAGTTCAG GCTTTGTCTT TACCCAATGA AGAAGGTGCC
    ACACCATTAG ACTTGGCTTT ACGTGCCGGA CACTCCAAGC TGGTTGAAGA CATCACAAAT
    TTCCAGGGCA GACGTTCCCC CGACCTCTCC CGAGTGCACC TCGGCGGACG TGCCTTCCTG
    CAGTATGTTC ACCCGTCGGG GACACTGACG CTGACGCTGA ACCACACAGC TGAGCATTTG
    TTGGAGGCAG ATATTAAACT CTTCAGGAAA TACTTTTTGG ACAGAGCCTT TCTCCTCAAG
    GCTCTTGACG ACCGAGAAGC TGGGCCAGAG GAGAGCCGGG GCATGCCCTC CCGGGCTGCC
    GCCACTGAGG AGGAGATTAG GAATTTGGTG ACCGGCAGGC CATCACCTGA AAAGGAGGAT
    GTAAAGCGTG TGGACAGCCC GGACGTTGAC CCCCATGAAC ACGACGGCCG GGACAGTCTT
    GATTTGGATC ACTCTTTCGA TGTCTTCAAA AAACCTAAGC ATCCCCCAGC GTTCTTTGCT
    GCAGGCGGGC TCTCTGATGT GCTGAATGGC GGCGATGAAG TTTACGCCAA CTGTATGGTG
    ATTGACCAGG TTGGTGATTT GGATATTAAC TACATCAATT TAGAAGGAAT CTCTACAGAC
    GCCTGTGCTG AATCCATGGG TTCTACTCTG GGGCCTCAGA ACTGCGAGCA TATCCTTGCT
    GCTGAAACCA GCCATGGGAA ATACCCACTA AGTGAGAACA AGGAAGTGGT CTCAGATACC
    GAAGCTCAGC AGTCCTTCCC ATCACCGTCT AGTTCATATG CTTCCAATTT TGATCTTTCC
    TTTGGTCTTC ATGGATTTGA GAAGGAACAA AGTCTCCTGA AGAAAAGAAG CTCCAGCCTG
    GATGCCTTGG ATGCCGACAG CGAAGGGGAA GGGCATTCTG AGCGGTCACA CATCTGCTAC
    ACGCCAGCAT CTCAGAGTTC CTCGAGGACT GGGATTCCCA GTGGGGATGA GCTGGACTCC
    TTCGAGACCG CCACCAAACC TGATTTTAGT ATCTCCAGGA CAGAGTCGCT TTCTTTATCA
    AGTAGCCTGC AGTTGAAGGA ATCATTGCTT CCTGGAGTCC GCTCACGTTC TTATTCCTGC
    TCATCACCCA AAATTTCTTT AGGAAAAACT CGATTGGTGC GTGATTTCAC AGTGTGCAAT
    TCTGGTGAAG AGCAAAGAGC CTTCGGCTTC CCGGAGCCCG CCAGGGAGAG GAGGATTCAA
    GAGGAAGAAT GGGATAAATA CATCATACCT GCCAAGTCAG AGTCTGAGAA ATACAAAGTG
    AGCCGGACCT TCAGCTTCCT CATGAACAGG ATGACAAGCC CTCGGAACAA ATCTAAGACG
    AAAAGCAAGG ATGCCAAAGA CAAAGAGAAA CCGAGCCGAC ATCAGTTTGT CCCCGGAACC
    TTCTCTGGGG TTCTCCAGTG TTCCGTTTGT GATAAAACGC TCCTGGGGAA GGAGTCATCC
    CAGTGTTCCA ACTGTAATGC AAACGTCCAC AAGGGTTGTA AGGACGCTGC ACCTCCGTGT
    ACCAAGAAAT TCCAGGAGAA ATATAACAAG AACAAACCGC AGACCATCCT TGGAAACTCT
    TCATTTCGAG ACATCCCGCA ACCCAGTCTC TCCTTGCACC CTTCTTCCTC CATGCCTATC
    GGATTGCCCA CTGGGAGAAA AGAAGCTTCT GCCCAGGCCC ATCCCTTGTC CAGAAGTGTT
    CCAGGCACTG CCTTGGAAAG CTTCAGAAGA TCGGGGCTAT CCCTGGAGTC TGAGAGTGAC
    GGGAACAGCT GGAGAAGCAG GTCTCATTCC GATGAGCTGT TACAGTCCAT GGGCTCTTCT
    CCCTCCACGG AATCCTTCTT AATGGAAGAT GTCGTGGACT CTTCTCTATG GAGTGACCTC
    AGCAGTGACG CCCAGGAGTT GGAAGCAGAA TCCTGGAGTC TGGTGGTGGA CCCCTCCTTT
    TGCAATAAGC AGGAGAAAGA TGTCATCAAA AGACAGGATG TCATTTTCGA GCTTATGCAA
    ACAGAGATGC ATCATATCCA GACCCTGCTC ATCATGTCTG AGATCTTCAG GAAAGGAATG
    AAGGAGGAGC TGCAGCTTGA TCACAGCACC GTGGATAAAA TCTTTCCCTG CTTAGATGAG
    TTACTTGAAA TCCACAGGCA TTTCTTTTAT AGTATGAAGG AGCGAAGGCA GGAGTCCTGT
    GCTGGTGGTG ACAGGAATTT TGTCATCAGC CGAATTGGAG ATATTCTAGT ACAGCAGTTT
    TCAGAAGAAA ATGCAAATCA AATGAAGAAA ATATATGGAG AATTCTGTAG CCATCACAAA
    GAAGCTGTTA GCCTCTATAA AGAACTCCAA CAGAATAAGA AGTTTCAGAA TTTTATTAAG
    CTTCGAAATA GTAATCTTTT GGCTCGACGC CGAGGAATCC CAGAATGCAT TCTGCTAGTC
    ACTCAGCGCA TCACAAAATA CCCTGTCCTG GTGGAACGGA TTTTGCAGTA CACAAAGGAA
    AGAAGTGAAG AACATCAAGA CTTGTGCAAA GCTCTGTGCT TAATTAAAGA CATGATTGCA
    GCAGTGGATT TAAAAGTCAG CGAGTATGAG AAAAACCAAA AATGGCTTGA GATCCTAAAT
    AAGATCGAAA ACAAAACCTA CACAAAGCTC AAAAATGGCC ACGTGTTTAG GAAGCAAGCA
    CTGATAAGTA AAGAAAGGAC TCTCTTATAT GATGGCCTTG TTTATTGGAA AACTGCTACG
    GGTCGTTTCA AAGATATCCT GGCTCTACTT CTAACTGATG TGCTGCTCTT TTTACAAGAA
    AAAGACCAGA AATACATCTT TGCAGCTGTT GATCAGAAGC CATCGGTTAT TTCCCTTCAG
    AAGCTCATTG CTAGAGAAGT CGCCAACGAG GAAAGAGGAA TGTTCCTGAT CAGTGCATCC
    TCTGCTGGTC CTGAGATGTA CGAAATTCAC ACCAACTCCA AGGAGGAACG AAATAACTGG
    ATGAGACGAA TCCAACAGGC CGTGGAAAGT TGCCCAGAAG AAGAAGGGGG AAGGACAAGT
    GAATCTGATG AAGAGAGGCG GAAAGCTGAA GCAAGAGTGG CCAAAATCCA GCAATGTCAA
    GAAATACTCA GTAACCAAGA CCAACAGATT TGCGCATATT TGGAGGAGAA GCTGCATATC
    TACGCTGAAC TTGGAGAACT GAGTGGATTC AAGGATGTCC ATCTAGAGCC CCACCTCCTC
    ATCAAACCTG ACCCAGGGGA GCCTCCCCAG GCAGCCTCAT TACTAGCAGC AGCCCTGAGA
    GAAGCGGAGA GCCTGCAGGT TGCCGTGAAG GCCTCACAAG CGGGTGATGC TCGCCCATCA
    CCTGAGGAGG GTTCTGCAGA ACCTGCGGTT GCAGATGCAC AGGGTCCTCC CGAGGAGCCA
    GGATCATCTC CAGCCTCACT AGTCACAGAA GGGACAGGAG GAAGAGGCTG TTGGGATGTG
    GATCCTGGCC CCCAGGGTGT GGGAACCGAC TTGGCGGTCT CTGATGCAGG GGAGAAGAGT
    CTTAAAAATC TGTGCTGCAG CCGGCAAAGT CCCTGGGGTG TCAGTCACCA TCACCAGCTG
    CAGCCCTGCA CAGGGACCAC AAGGGGGAGG GCCAGCTCCA CGAAAAGTAG GACACTACAC
    AGCTCAGGCT TGTATCAGGC TTCGTGGTTC TTGGGAGTGG AATATAGAAG TTTCACGGTT
    TCTTCACAGT CAGAGATTAT ACAAGCGATC CAGAATTTAA CCCGACTCCT CTATAGCCTT
    CAGGCCGCGC TGACCATCCA GGACAGCCAC ATCCAGGTGC ACAGGCTGCT GCTGCAGCAG
    GCTGGCCTGC CGGCGTCCCA AGAGGAGCAC GGCGAGCTGG CGAGGCAGCG GGAGGAGCTG
    GCCGCCCTGC CCGGGCTCCA GTGGCAGCTG CAGCAGGAGC GGCGGCGCGG CCAGCGCACC
    TGGGAGCGGC AGCAGCGCGA GCAGGAGGCC AGGGCCTGCC GGCTGCGGGA GCGCGAGCAG
    GGCTGCCGCG GCCGCGAGGA GCTGCTGCTC AGGGCCCGCG GGGAGCTGGA CCGGCAGCTG
    CGGGAGCACC GGCAGGACCT GGAGCGGCTG CGGGAGGGCC AGCGCCTGGT GGAGAGTGCG
    AGGGCCCGCA CGCGCGCCCA GCACAGCCTG CTGCGTGGCC CGAGGCCCGG GCGCCAGAGC
    AGCCTGCCCG CGGCCTTGCC TCCGGGCAGC GGCGAGGTGA TGGAACTTAA TCGATCCGAG
    AGCTTGTGTC ATGAAAATTC ATTCTTCATT AATGAAGCTT TAGTACAAAT GTCACTTAAC
    ACTTACAACA AATCAACTCC ATCAGGTGTC CACCAGGAAG CCGCTTATCC CCAGAATATA
    GCTAATTCGG ACTTGGTAAG GACTAGTGAA AGTCCCGTAG ACCTCCAGTT GGACATTTCT
    CAGCCCTTGG ATGTCAACCA CGAACTGTGG ACAGCCGCTG GGTCCTGTCA TCAGATACCC
    CCTCTCCACG AAAGCAGCAA GGATTCTTGT AAAAATGGAA GAATTTACAT CAGTCAGCTA
    TGTTTCCAGA GCCATTCGTC AGAAATGTTT TGGCGACCAG ACTTGACCAT CTCAGCTTGG
    GATAGAGAGC TTCCGAATCT GCAGAACTTC CAACCGCTGT TGGTTGCATT ATGTAGATGG
    TTGCGTTACG TCCGTACATC CCCGGGAGAT CTTGCCGTCA TCCTCGAGTC CCCTGAGCAG
    GTTTTTCAGC TAGAAAGAAA ATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35381
    Clone ID Related Accession (Same CDS sequence) XM_014109060.1 , XM_014109060.2
    Accession Version XM_014109060.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5280bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product rho guanine nucleotide exchange factor 28 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGAGTTGA GCTGCAGCGA AGTACCTCTT TATGGGCAGA TGACCGTCTA TGCAAAGTTT 
    GGGAAAAATG TTTATCTTCC TAAAGATGCC GAGTTTTACT TTATTTATAA TGGATCTCAT
    CAGAGACACG TCGTGATTGC AAAACGCACT GAGGATAATG TTCTCCAGTC CAGCATTCCA
    GGGCATGGGC TTCAGGAGAC GGTGACGGTG TCTGTATGCC TCTGCTCAGA AGGTTACTCC
    CCAGTGACCA TGGGCTCCGG CTCAGTGACC TACGTGGACA ACATGGCTTG CAGGCTGGCT
    CGTTTGCTGG TCACCCAGGC TGATCGCCTC ACAGCCAGTA GTCATCAGAC CTTTCTGACT
    CCATTTGCCC TGACGGTGGG AGCATTGCCT GCTTTGGACG AGGAGCTGGT GCTGGCTCTG
    ACCCATCTGG AGTTGCCTCT TGAGTGGAGT GTCTTGGGAA ATTCTTCACT TGAAGTTTCT
    TTATGCAGAG AGTCTCTTCT ACACCTGGCT ATGAGATGGG GCCTGCCTAA GCTCTCTCAT
    TTCCTCTTGT GCCTCCCTGG GGGAGTTCAG GCTTTGTCTT TACCCAATGA AGAAGGTGCC
    ACACCATTAG ACTTGGCTTT ACGTGCCGGA CACTCCAAGC TGGTTGAAGA CATCACAAAT
    TTCCAGGGCA GACGTTCCCC CGACCTCTCC CGAGTGCACC TCGGCGGACG TGCCTTCCTG
    CAGTATGTTC ACCCGTCGGG GACACTGACG CTGACGCTGA ACCACACAGC TGAGCATTTG
    TTGGAGGCAG ATATTAAACT CTTCAGGAAA TACTTTTTGG ACAGAGCCTT TCTCCTCAAG
    GCTCTTGACG ACCGAGAAGC TGGGCCAGAG GAGAGCCGGG GCATGCCCTC CCGGGCTGCC
    GCCACTGAGG AGGGTACGCG AGCTGCCTCC CCCGCGCCCA GGAACCCAGA AGAGATTAGG
    AATTTGGTGA CCGGCAGGCC ATCACCTGAA AAGGAGGATG TAAAGCGTGT GGACAGCCCG
    GACGTTGACC CCCATGAACA CGACGGCCGG GACAGTCTTG ATTTGGATCA CTCTTTCGAT
    GTCTTCAAAA AACCTAAGCA TCCCCCAGCG TTCTTTGCTG CAGGCGGGCT CTCTGATGTG
    CTGAATGGCG GCGATGAAGT TTACGCCAAC TGTATGGTGA TTGACCAGGT TGGTGATTTG
    GATATTAACT ACATCAATTT AGAAGGAATC TCTACAGACG CCTGTGCTGA ATCCATGGGT
    TCTACTCTGG GGCCTCAGAA CTGCGAGCAT ATCCTTGCTG CTGAAACCAG CCATGGGAAA
    TACCCACTAA GTGAGAACAA GGAAGTGGTC TCAGATACCG AAGCTCAGCA GTCCTTCCCA
    TCACCGTCTA GTTCATATGC TTCCAATTTT GATCTTTCCT TTGGTCTTCA TGGATTTGAG
    AAGGAACAAA GTCTCCTGAA GAAAAGAAGC TCCAGCCTGG ATGCCTTGGA TGCCGACAGC
    GAAGGGGAAG GGCATTCTGA GCGGTCACAC ATCTGCTACA CGCCAGCATC TCAGAGTTCC
    TCGAGGACTG GGATTCCCAG TGGGGATGAG CTGGACTCCT TCGAGACCGC CACCAAACCT
    GATTTTAGTA TCTCCAGGAC AGAGTCGCTT TCTTTATCAA GTAGCCTGCA GTTGAAGGAA
    TCATTGCTTC CTGGAGTCCG CTCACGTTCT TATTCCTGCT CATCACCCAA AATTTCTTTA
    GGAAAAACTC GATTGGTGCG TGATTTCACA GTGTGCAATT CTGGTGAAGA GCAAAGAGCC
    TTCGGCTTCC CGGAGCCCGC CAGGGAGAGG AGGATTCAAG AGGAAGAATG GGATAAATAC
    ATCATACCTG CCAAGTCAGA GTCTGAGAAA TACAAAGTGA GCCGGACCTT CAGCTTCCTC
    ATGAACAGGA TGACAAGCCC TCGGAACAAA TCTAAGACGA AAAGCAAGGA TGCCAAAGAC
    AAAGAGAAAC CGAGCCGACA TCAGTTTGTC CCCGGAACCT TCTCTGGGGT TCTCCAGTGT
    TCCGTTTGTG ATAAAACGCT CCTGGGGAAG GAGTCATCCC AGTGTTCCAA CTGTAATGCA
    AACGTCCACA AGGGTTGTAA GGACGCTGCA CCTCCGTGTA CCAAGAAATT CCAGGAGAAA
    TATAACAAGA ACAAACCGCA GACCATCCTT GGAAACTCTT CATTTCGAGA CATCCCGCAA
    CCCAGTCTCT CCTTGCACCC TTCTTCCTCC ATGCCTATCG GATTGCCCAC TGGGAGAAAA
    GAAGCTTCTG CCCAGGCCCA TCCCTTGTCC AGAAGTGTTC CAGGCACTGC CTTGGAAAGC
    TTCAGAAGAT CGGGGCTATC CCTGGAGTCT GAGAGTGACG GGAACAGCTG GAGAAGCAGG
    TCTCATTCCG ATGAGCTGTT ACAGTCCATG GGCTCTTCTC CCTCCACGGA ATCCTTCTTA
    ATGGAAGATG TCGTGGACTC TTCTCTATGG AGTGACCTCA GCAGTGACGC CCAGGAGTTG
    GAAGCAGAAT CCTGGAGTCT GGTGGTGGAC CCCTCCTTTT GCAATAAGCA GGAGAAAGAT
    GTCATCAAAA GACAGGATGT CATTTTCGAG CTTATGCAAA CAGAGATGCA TCATATCCAG
    ACCCTGCTCA TCATGTCTGA GATCTTCAGG AAAGGAATGA AGGAGGAGCT GCAGCTTGAT
    CACAGCACCG TGGATAAAAT CTTTCCCTGC TTAGATGAGT TACTTGAAAT CCACAGGCAT
    TTCTTTTATA GTATGAAGGA GCGAAGGCAG GAGTCCTGTG CTGGTGGTGA CAGGAATTTT
    GTCATCAGCC GAATTGGAGA TATTCTAGTA CAGCAGTTTT CAGAAGAAAA TGCAAATCAA
    ATGAAGAAAA TATATGGAGA ATTCTGTAGC CATCACAAAG AAGCTGTTAG CCTCTATAAA
    GAACTCCAAC AGAATAAGAA GTTTCAGAAT TTTATTAAGC TTCGAAATAG TAATCTTTTG
    GCTCGACGCC GAGGAATCCC AGAATGCATT CTGCTAGTCA CTCAGCGCAT CACAAAATAC
    CCTGTCCTGG TGGAACGGAT TTTGCAGTAC ACAAAGGAAA GAAGTGAAGA ACATCAAGAC
    TTGTGCAAAG CTCTGTGCTT AATTAAAGAC ATGATTGCAG CAGTGGATTT AAAAGTCAGC
    GAGTATGAGA AAAACCAAAA ATGGCTTGAG ATCCTAAATA AGATCGAAAA CAAAACCTAC
    ACAAAGCTCA AAAATGGCCA CGTGTTTAGG AAGCAAGCAC TGATAAGTAA AGAAAGGACT
    CTCTTATATG ATGGCCTTGT TTATTGGAAA ACTGCTACGG GTCGTTTCAA AGATATCCTG
    GCTCTACTTC TAACTGATGT GCTGCTCTTT TTACAAGAAA AAGACCAGAA ATACATCTTT
    GCAGCTGTTG ATCAGAAGCC ATCGGTTATT TCCCTTCAGA AGCTCATTGC TAGAGAAGTC
    GCCAACGAGG AAAGAGGAAT GTTCCTGATC AGTGCATCCT CTGCTGGTCC TGAGATGTAC
    GAAATTCACA CCAACTCCAA GGAGGAACGA AATAACTGGA TGAGACGAAT CCAACAGGCC
    GTGGAAAGTT GCCCAGAAGA AGAAGGGGGA AGGACAAGTG AATCTGATGA AGAGAGGCGG
    AAAGCTGAAG CAAGAGTGGC CAAAATCCAG CAATGTCAAG AAATACTCAG TAACCAAGAC
    CAACAGATTT GCGCATATTT GGAGGAGAAG CTGCATATCT ACGCTGAACT TGGAGAACTG
    AGTGGATTCA AGGATGTCCA TCTAGAGCCC CACCTCCTCA TCAAACCTGA CCCAGGGGAG
    CCTCCCCAGG CAGCCTCATT ACTAGCAGCA GCCCTGAGAG AAGCGGAGAG CCTGCAGGTT
    GCCGTGAAGG CCTCACAAGC GGGTGATGCT CGCCCATCAC CTGAGGAGGG TTCTGCAGAA
    CCTGCGGTTG CAGATGCACA GGGTCCTCCC GAGGAGCCAG GATCATCTCC AGCCTCACTA
    GTCACAGAAG GGACAGGAGG AAGAGGCTGT TGGGATGTGG ATCCTGGCCC CCAGGGTGTG
    GGAACCGACT TGGCGGTCTC TGATGCAGGG GAGAAGAGTC TTAAAAATCT GTGCTGCAGC
    CGGCAAAGTC CCTGGGGTGT CAGTCACCAT CACCAGCTGC AGCCCTGCAC AGGGACCACA
    AGGGGGAGGG CCAGCTCCAC GAAAAGTAGG ACACTACACA GCTCAGGCTT GTATCAGGCT
    TCGTGGTTCT TGGGAGTGGA ATATAGAAGT TTCACGGTTT CTTCACAGTC AGAGATTATA
    CAAGCGATCC AGAATTTAAC CCGACTCCTC TATAGCCTTC AGGCCGCGCT GACCATCCAG
    GACAGCCACA TCCAGGTGCA CAGGCTGCTG CTGCAGCAGG CTGGCCTGCC GGCGTCCCAA
    GAGGAGCACG GCGAGCTGGC GAGGCAGCGG GAGGAGCTGG CCGCCCTGCC CGGGCTCCAG
    TGGCAGCTGC AGCAGGAGCG GCGGCGCGGC CAGCGCACCT GGGAGCGGCA GCAGCGCGAG
    CAGGAGGCCA GGGCCTGCCG GCTGCGGGAG CGCGAGCAGG GCTGCCGCGG CCGCGAGGAG
    CTGCTGCTCA GGGCCCGCGG GGAGCTGGAC CGGCAGCTGC GGGAGCACCG GCAGGACCTG
    GAGCGGCTGC GGGAGGGCCA GCGCCTGGTG GAGAGTGCGA GGGCCCGCAC GCGCGCCCAG
    CACAGCCTGC TGCGTGGCCC GAGGCCCGGG CGCCAGAGCA GCCTGCCCGC GGCCTTGCCT
    CCGGGCAGCG GCGAGGTGAT GGAACTTAAT CGATCCGAGA GCTTGTGTCA TGAAAATTCA
    TTCTTCATTA ATGAAGCTTT AGTACAAATG TCACTTAACA CTTACAACAA ATCAACTCCA
    TCAGGTGTCC ACCAGGAAGC CGCTTATCCC CAGAATATAG CTAATTCGGA CTTGGTAAGG
    ACTAGTGAAA GTCCCGTAGA CCTCCAGTTG GACATTTCTC AGCCCTTGGA TGTCAACCAC
    GAACTGTGGA CAGCCGCTGG GTCCTGTCAT CAGATACCCC CTCTCCACGA AAGCAGCAAG
    GATTCTTGTA AAAATGGCCT GCGCACCCCT CACCCGGGGC CCTCGGCCTC CCCGGAGCCC
    AGTGCACGCA GCTCCCGGCT GCAGACGGGG GCGGCAGAAG CAGCGCACAA CCTACAGACG
    CGGACCGGAC AAGATGCAGA GACCGGAGAC GCGGCTGAAG AAAATATCGT TTACCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013964535.1
    CDS12..5291
    Translation

    Target ORF information:

    RefSeq Version XM_014109060.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 28 (ARHGEF28), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109060.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGAGTTGA GCTGCAGCGA AGTACCTCTT TATGGGCAGA TGACCGTCTA TGCAAAGTTT 
    GGGAAAAATG TTTATCTTCC TAAAGATGCC GAGTTTTACT TTATTTATAA TGGATCTCAT
    CAGAGACACG TCGTGATTGC AAAACGCACT GAGGATAATG TTCTCCAGTC CAGCATTCCA
    GGGCATGGGC TTCAGGAGAC GGTGACGGTG TCTGTATGCC TCTGCTCAGA AGGTTACTCC
    CCAGTGACCA TGGGCTCCGG CTCAGTGACC TACGTGGACA ACATGGCTTG CAGGCTGGCT
    CGTTTGCTGG TCACCCAGGC TGATCGCCTC ACAGCCAGTA GTCATCAGAC CTTTCTGACT
    CCATTTGCCC TGACGGTGGG AGCATTGCCT GCTTTGGACG AGGAGCTGGT GCTGGCTCTG
    ACCCATCTGG AGTTGCCTCT TGAGTGGAGT GTCTTGGGAA ATTCTTCACT TGAAGTTTCT
    TTATGCAGAG AGTCTCTTCT ACACCTGGCT ATGAGATGGG GCCTGCCTAA GCTCTCTCAT
    TTCCTCTTGT GCCTCCCTGG GGGAGTTCAG GCTTTGTCTT TACCCAATGA AGAAGGTGCC
    ACACCATTAG ACTTGGCTTT ACGTGCCGGA CACTCCAAGC TGGTTGAAGA CATCACAAAT
    TTCCAGGGCA GACGTTCCCC CGACCTCTCC CGAGTGCACC TCGGCGGACG TGCCTTCCTG
    CAGTATGTTC ACCCGTCGGG GACACTGACG CTGACGCTGA ACCACACAGC TGAGCATTTG
    TTGGAGGCAG ATATTAAACT CTTCAGGAAA TACTTTTTGG ACAGAGCCTT TCTCCTCAAG
    GCTCTTGACG ACCGAGAAGC TGGGCCAGAG GAGAGCCGGG GCATGCCCTC CCGGGCTGCC
    GCCACTGAGG AGGGTACGCG AGCTGCCTCC CCCGCGCCCA GGAACCCAGA AGAGATTAGG
    AATTTGGTGA CCGGCAGGCC ATCACCTGAA AAGGAGGATG TAAAGCGTGT GGACAGCCCG
    GACGTTGACC CCCATGAACA CGACGGCCGG GACAGTCTTG ATTTGGATCA CTCTTTCGAT
    GTCTTCAAAA AACCTAAGCA TCCCCCAGCG TTCTTTGCTG CAGGCGGGCT CTCTGATGTG
    CTGAATGGCG GCGATGAAGT TTACGCCAAC TGTATGGTGA TTGACCAGGT TGGTGATTTG
    GATATTAACT ACATCAATTT AGAAGGAATC TCTACAGACG CCTGTGCTGA ATCCATGGGT
    TCTACTCTGG GGCCTCAGAA CTGCGAGCAT ATCCTTGCTG CTGAAACCAG CCATGGGAAA
    TACCCACTAA GTGAGAACAA GGAAGTGGTC TCAGATACCG AAGCTCAGCA GTCCTTCCCA
    TCACCGTCTA GTTCATATGC TTCCAATTTT GATCTTTCCT TTGGTCTTCA TGGATTTGAG
    AAGGAACAAA GTCTCCTGAA GAAAAGAAGC TCCAGCCTGG ATGCCTTGGA TGCCGACAGC
    GAAGGGGAAG GGCATTCTGA GCGGTCACAC ATCTGCTACA CGCCAGCATC TCAGAGTTCC
    TCGAGGACTG GGATTCCCAG TGGGGATGAG CTGGACTCCT TCGAGACCGC CACCAAACCT
    GATTTTAGTA TCTCCAGGAC AGAGTCGCTT TCTTTATCAA GTAGCCTGCA GTTGAAGGAA
    TCATTGCTTC CTGGAGTCCG CTCACGTTCT TATTCCTGCT CATCACCCAA AATTTCTTTA
    GGAAAAACTC GATTGGTGCG TGATTTCACA GTGTGCAATT CTGGTGAAGA GCAAAGAGCC
    TTCGGCTTCC CGGAGCCCGC CAGGGAGAGG AGGATTCAAG AGGAAGAATG GGATAAATAC
    ATCATACCTG CCAAGTCAGA GTCTGAGAAA TACAAAGTGA GCCGGACCTT CAGCTTCCTC
    ATGAACAGGA TGACAAGCCC TCGGAACAAA TCTAAGACGA AAAGCAAGGA TGCCAAAGAC
    AAAGAGAAAC CGAGCCGACA TCAGTTTGTC CCCGGAACCT TCTCTGGGGT TCTCCAGTGT
    TCCGTTTGTG ATAAAACGCT CCTGGGGAAG GAGTCATCCC AGTGTTCCAA CTGTAATGCA
    AACGTCCACA AGGGTTGTAA GGACGCTGCA CCTCCGTGTA CCAAGAAATT CCAGGAGAAA
    TATAACAAGA ACAAACCGCA GACCATCCTT GGAAACTCTT CATTTCGAGA CATCCCGCAA
    CCCAGTCTCT CCTTGCACCC TTCTTCCTCC ATGCCTATCG GATTGCCCAC TGGGAGAAAA
    GAAGCTTCTG CCCAGGCCCA TCCCTTGTCC AGAAGTGTTC CAGGCACTGC CTTGGAAAGC
    TTCAGAAGAT CGGGGCTATC CCTGGAGTCT GAGAGTGACG GGAACAGCTG GAGAAGCAGG
    TCTCATTCCG ATGAGCTGTT ACAGTCCATG GGCTCTTCTC CCTCCACGGA ATCCTTCTTA
    ATGGAAGATG TCGTGGACTC TTCTCTATGG AGTGACCTCA GCAGTGACGC CCAGGAGTTG
    GAAGCAGAAT CCTGGAGTCT GGTGGTGGAC CCCTCCTTTT GCAATAAGCA GGAGAAAGAT
    GTCATCAAAA GACAGGATGT CATTTTCGAG CTTATGCAAA CAGAGATGCA TCATATCCAG
    ACCCTGCTCA TCATGTCTGA GATCTTCAGG AAAGGAATGA AGGAGGAGCT GCAGCTTGAT
    CACAGCACCG TGGATAAAAT CTTTCCCTGC TTAGATGAGT TACTTGAAAT CCACAGGCAT
    TTCTTTTATA GTATGAAGGA GCGAAGGCAG GAGTCCTGTG CTGGTGGTGA CAGGAATTTT
    GTCATCAGCC GAATTGGAGA TATTCTAGTA CAGCAGTTTT CAGAAGAAAA TGCAAATCAA
    ATGAAGAAAA TATATGGAGA ATTCTGTAGC CATCACAAAG AAGCTGTTAG CCTCTATAAA
    GAACTCCAAC AGAATAAGAA GTTTCAGAAT TTTATTAAGC TTCGAAATAG TAATCTTTTG
    GCTCGACGCC GAGGAATCCC AGAATGCATT CTGCTAGTCA CTCAGCGCAT CACAAAATAC
    CCTGTCCTGG TGGAACGGAT TTTGCAGTAC ACAAAGGAAA GAAGTGAAGA ACATCAAGAC
    TTGTGCAAAG CTCTGTGCTT AATTAAAGAC ATGATTGCAG CAGTGGATTT AAAAGTCAGC
    GAGTATGAGA AAAACCAAAA ATGGCTTGAG ATCCTAAATA AGATCGAAAA CAAAACCTAC
    ACAAAGCTCA AAAATGGCCA CGTGTTTAGG AAGCAAGCAC TGATAAGTAA AGAAAGGACT
    CTCTTATATG ATGGCCTTGT TTATTGGAAA ACTGCTACGG GTCGTTTCAA AGATATCCTG
    GCTCTACTTC TAACTGATGT GCTGCTCTTT TTACAAGAAA AAGACCAGAA ATACATCTTT
    GCAGCTGTTG ATCAGAAGCC ATCGGTTATT TCCCTTCAGA AGCTCATTGC TAGAGAAGTC
    GCCAACGAGG AAAGAGGAAT GTTCCTGATC AGTGCATCCT CTGCTGGTCC TGAGATGTAC
    GAAATTCACA CCAACTCCAA GGAGGAACGA AATAACTGGA TGAGACGAAT CCAACAGGCC
    GTGGAAAGTT GCCCAGAAGA AGAAGGGGGA AGGACAAGTG AATCTGATGA AGAGAGGCGG
    AAAGCTGAAG CAAGAGTGGC CAAAATCCAG CAATGTCAAG AAATACTCAG TAACCAAGAC
    CAACAGATTT GCGCATATTT GGAGGAGAAG CTGCATATCT ACGCTGAACT TGGAGAACTG
    AGTGGATTCA AGGATGTCCA TCTAGAGCCC CACCTCCTCA TCAAACCTGA CCCAGGGGAG
    CCTCCCCAGG CAGCCTCATT ACTAGCAGCA GCCCTGAGAG AAGCGGAGAG CCTGCAGGTT
    GCCGTGAAGG CCTCACAAGC GGGTGATGCT CGCCCATCAC CTGAGGAGGG TTCTGCAGAA
    CCTGCGGTTG CAGATGCACA GGGTCCTCCC GAGGAGCCAG GATCATCTCC AGCCTCACTA
    GTCACAGAAG GGACAGGAGG AAGAGGCTGT TGGGATGTGG ATCCTGGCCC CCAGGGTGTG
    GGAACCGACT TGGCGGTCTC TGATGCAGGG GAGAAGAGTC TTAAAAATCT GTGCTGCAGC
    CGGCAAAGTC CCTGGGGTGT CAGTCACCAT CACCAGCTGC AGCCCTGCAC AGGGACCACA
    AGGGGGAGGG CCAGCTCCAC GAAAAGTAGG ACACTACACA GCTCAGGCTT GTATCAGGCT
    TCGTGGTTCT TGGGAGTGGA ATATAGAAGT TTCACGGTTT CTTCACAGTC AGAGATTATA
    CAAGCGATCC AGAATTTAAC CCGACTCCTC TATAGCCTTC AGGCCGCGCT GACCATCCAG
    GACAGCCACA TCCAGGTGCA CAGGCTGCTG CTGCAGCAGG CTGGCCTGCC GGCGTCCCAA
    GAGGAGCACG GCGAGCTGGC GAGGCAGCGG GAGGAGCTGG CCGCCCTGCC CGGGCTCCAG
    TGGCAGCTGC AGCAGGAGCG GCGGCGCGGC CAGCGCACCT GGGAGCGGCA GCAGCGCGAG
    CAGGAGGCCA GGGCCTGCCG GCTGCGGGAG CGCGAGCAGG GCTGCCGCGG CCGCGAGGAG
    CTGCTGCTCA GGGCCCGCGG GGAGCTGGAC CGGCAGCTGC GGGAGCACCG GCAGGACCTG
    GAGCGGCTGC GGGAGGGCCA GCGCCTGGTG GAGAGTGCGA GGGCCCGCAC GCGCGCCCAG
    CACAGCCTGC TGCGTGGCCC GAGGCCCGGG CGCCAGAGCA GCCTGCCCGC GGCCTTGCCT
    CCGGGCAGCG GCGAGGTGAT GGAACTTAAT CGATCCGAGA GCTTGTGTCA TGAAAATTCA
    TTCTTCATTA ATGAAGCTTT AGTACAAATG TCACTTAACA CTTACAACAA ATCAACTCCA
    TCAGGTGTCC ACCAGGAAGC CGCTTATCCC CAGAATATAG CTAATTCGGA CTTGGTAAGG
    ACTAGTGAAA GTCCCGTAGA CCTCCAGTTG GACATTTCTC AGCCCTTGGA TGTCAACCAC
    GAACTGTGGA CAGCCGCTGG GTCCTGTCAT CAGATACCCC CTCTCCACGA AAGCAGCAAG
    GATTCTTGTA AAAATGGCCT GCGCACCCCT CACCCGGGGC CCTCGGCCTC CCCGGAGCCC
    AGTGCACGCA GCTCCCGGCT GCAGACGGGG GCGGCAGAAG CAGCGCACAA CCTACAGACG
    CGGACCGGAC AAGATGCAGA GACCGGAGAC GCGGCTGAAG AAAATATCGT TTACCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35381
    Clone ID Related Accession (Same CDS sequence) XM_014109060.1 , XM_014109060.2
    Accession Version XM_014109060.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5280bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rho guanine nucleotide exchange factor 28 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109060.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGAGTTGA GCTGCAGCGA AGTACCTCTT TATGGGCAGA TGACCGTCTA TGCAAAGTTT 
    GGGAAAAATG TTTATCTTCC TAAAGATGCC GAGTTTTACT TTATTTATAA TGGATCTCAT
    CAGAGACACG TCGTGATTGC AAAACGCACT GAGGATAATG TTCTCCAGTC CAGCATTCCA
    GGGCATGGGC TTCAGGAGAC GGTGACGGTG TCTGTATGCC TCTGCTCAGA AGGTTACTCC
    CCAGTGACCA TGGGCTCCGG CTCAGTGACC TACGTGGACA ACATGGCTTG CAGGCTGGCT
    CGTTTGCTGG TCACCCAGGC TGATCGCCTC ACAGCCAGTA GTCATCAGAC CTTTCTGACT
    CCATTTGCCC TGACGGTGGG AGCATTGCCT GCTTTGGACG AGGAGCTGGT GCTGGCTCTG
    ACCCATCTGG AGTTGCCTCT TGAGTGGAGT GTCTTGGGAA ATTCTTCACT TGAAGTTTCT
    TTATGCAGAG AGTCTCTTCT ACACCTGGCT ATGAGATGGG GCCTGCCTAA GCTCTCTCAT
    TTCCTCTTGT GCCTCCCTGG GGGAGTTCAG GCTTTGTCTT TACCCAATGA AGAAGGTGCC
    ACACCATTAG ACTTGGCTTT ACGTGCCGGA CACTCCAAGC TGGTTGAAGA CATCACAAAT
    TTCCAGGGCA GACGTTCCCC CGACCTCTCC CGAGTGCACC TCGGCGGACG TGCCTTCCTG
    CAGTATGTTC ACCCGTCGGG GACACTGACG CTGACGCTGA ACCACACAGC TGAGCATTTG
    TTGGAGGCAG ATATTAAACT CTTCAGGAAA TACTTTTTGG ACAGAGCCTT TCTCCTCAAG
    GCTCTTGACG ACCGAGAAGC TGGGCCAGAG GAGAGCCGGG GCATGCCCTC CCGGGCTGCC
    GCCACTGAGG AGGGTACGCG AGCTGCCTCC CCCGCGCCCA GGAACCCAGA AGAGATTAGG
    AATTTGGTGA CCGGCAGGCC ATCACCTGAA AAGGAGGATG TAAAGCGTGT GGACAGCCCG
    GACGTTGACC CCCATGAACA CGACGGCCGG GACAGTCTTG ATTTGGATCA CTCTTTCGAT
    GTCTTCAAAA AACCTAAGCA TCCCCCAGCG TTCTTTGCTG CAGGCGGGCT CTCTGATGTG
    CTGAATGGCG GCGATGAAGT TTACGCCAAC TGTATGGTGA TTGACCAGGT TGGTGATTTG
    GATATTAACT ACATCAATTT AGAAGGAATC TCTACAGACG CCTGTGCTGA ATCCATGGGT
    TCTACTCTGG GGCCTCAGAA CTGCGAGCAT ATCCTTGCTG CTGAAACCAG CCATGGGAAA
    TACCCACTAA GTGAGAACAA GGAAGTGGTC TCAGATACCG AAGCTCAGCA GTCCTTCCCA
    TCACCGTCTA GTTCATATGC TTCCAATTTT GATCTTTCCT TTGGTCTTCA TGGATTTGAG
    AAGGAACAAA GTCTCCTGAA GAAAAGAAGC TCCAGCCTGG ATGCCTTGGA TGCCGACAGC
    GAAGGGGAAG GGCATTCTGA GCGGTCACAC ATCTGCTACA CGCCAGCATC TCAGAGTTCC
    TCGAGGACTG GGATTCCCAG TGGGGATGAG CTGGACTCCT TCGAGACCGC CACCAAACCT
    GATTTTAGTA TCTCCAGGAC AGAGTCGCTT TCTTTATCAA GTAGCCTGCA GTTGAAGGAA
    TCATTGCTTC CTGGAGTCCG CTCACGTTCT TATTCCTGCT CATCACCCAA AATTTCTTTA
    GGAAAAACTC GATTGGTGCG TGATTTCACA GTGTGCAATT CTGGTGAAGA GCAAAGAGCC
    TTCGGCTTCC CGGAGCCCGC CAGGGAGAGG AGGATTCAAG AGGAAGAATG GGATAAATAC
    ATCATACCTG CCAAGTCAGA GTCTGAGAAA TACAAAGTGA GCCGGACCTT CAGCTTCCTC
    ATGAACAGGA TGACAAGCCC TCGGAACAAA TCTAAGACGA AAAGCAAGGA TGCCAAAGAC
    AAAGAGAAAC CGAGCCGACA TCAGTTTGTC CCCGGAACCT TCTCTGGGGT TCTCCAGTGT
    TCCGTTTGTG ATAAAACGCT CCTGGGGAAG GAGTCATCCC AGTGTTCCAA CTGTAATGCA
    AACGTCCACA AGGGTTGTAA GGACGCTGCA CCTCCGTGTA CCAAGAAATT CCAGGAGAAA
    TATAACAAGA ACAAACCGCA GACCATCCTT GGAAACTCTT CATTTCGAGA CATCCCGCAA
    CCCAGTCTCT CCTTGCACCC TTCTTCCTCC ATGCCTATCG GATTGCCCAC TGGGAGAAAA
    GAAGCTTCTG CCCAGGCCCA TCCCTTGTCC AGAAGTGTTC CAGGCACTGC CTTGGAAAGC
    TTCAGAAGAT CGGGGCTATC CCTGGAGTCT GAGAGTGACG GGAACAGCTG GAGAAGCAGG
    TCTCATTCCG ATGAGCTGTT ACAGTCCATG GGCTCTTCTC CCTCCACGGA ATCCTTCTTA
    ATGGAAGATG TCGTGGACTC TTCTCTATGG AGTGACCTCA GCAGTGACGC CCAGGAGTTG
    GAAGCAGAAT CCTGGAGTCT GGTGGTGGAC CCCTCCTTTT GCAATAAGCA GGAGAAAGAT
    GTCATCAAAA GACAGGATGT CATTTTCGAG CTTATGCAAA CAGAGATGCA TCATATCCAG
    ACCCTGCTCA TCATGTCTGA GATCTTCAGG AAAGGAATGA AGGAGGAGCT GCAGCTTGAT
    CACAGCACCG TGGATAAAAT CTTTCCCTGC TTAGATGAGT TACTTGAAAT CCACAGGCAT
    TTCTTTTATA GTATGAAGGA GCGAAGGCAG GAGTCCTGTG CTGGTGGTGA CAGGAATTTT
    GTCATCAGCC GAATTGGAGA TATTCTAGTA CAGCAGTTTT CAGAAGAAAA TGCAAATCAA
    ATGAAGAAAA TATATGGAGA ATTCTGTAGC CATCACAAAG AAGCTGTTAG CCTCTATAAA
    GAACTCCAAC AGAATAAGAA GTTTCAGAAT TTTATTAAGC TTCGAAATAG TAATCTTTTG
    GCTCGACGCC GAGGAATCCC AGAATGCATT CTGCTAGTCA CTCAGCGCAT CACAAAATAC
    CCTGTCCTGG TGGAACGGAT TTTGCAGTAC ACAAAGGAAA GAAGTGAAGA ACATCAAGAC
    TTGTGCAAAG CTCTGTGCTT AATTAAAGAC ATGATTGCAG CAGTGGATTT AAAAGTCAGC
    GAGTATGAGA AAAACCAAAA ATGGCTTGAG ATCCTAAATA AGATCGAAAA CAAAACCTAC
    ACAAAGCTCA AAAATGGCCA CGTGTTTAGG AAGCAAGCAC TGATAAGTAA AGAAAGGACT
    CTCTTATATG ATGGCCTTGT TTATTGGAAA ACTGCTACGG GTCGTTTCAA AGATATCCTG
    GCTCTACTTC TAACTGATGT GCTGCTCTTT TTACAAGAAA AAGACCAGAA ATACATCTTT
    GCAGCTGTTG ATCAGAAGCC ATCGGTTATT TCCCTTCAGA AGCTCATTGC TAGAGAAGTC
    GCCAACGAGG AAAGAGGAAT GTTCCTGATC AGTGCATCCT CTGCTGGTCC TGAGATGTAC
    GAAATTCACA CCAACTCCAA GGAGGAACGA AATAACTGGA TGAGACGAAT CCAACAGGCC
    GTGGAAAGTT GCCCAGAAGA AGAAGGGGGA AGGACAAGTG AATCTGATGA AGAGAGGCGG
    AAAGCTGAAG CAAGAGTGGC CAAAATCCAG CAATGTCAAG AAATACTCAG TAACCAAGAC
    CAACAGATTT GCGCATATTT GGAGGAGAAG CTGCATATCT ACGCTGAACT TGGAGAACTG
    AGTGGATTCA AGGATGTCCA TCTAGAGCCC CACCTCCTCA TCAAACCTGA CCCAGGGGAG
    CCTCCCCAGG CAGCCTCATT ACTAGCAGCA GCCCTGAGAG AAGCGGAGAG CCTGCAGGTT
    GCCGTGAAGG CCTCACAAGC GGGTGATGCT CGCCCATCAC CTGAGGAGGG TTCTGCAGAA
    CCTGCGGTTG CAGATGCACA GGGTCCTCCC GAGGAGCCAG GATCATCTCC AGCCTCACTA
    GTCACAGAAG GGACAGGAGG AAGAGGCTGT TGGGATGTGG ATCCTGGCCC CCAGGGTGTG
    GGAACCGACT TGGCGGTCTC TGATGCAGGG GAGAAGAGTC TTAAAAATCT GTGCTGCAGC
    CGGCAAAGTC CCTGGGGTGT CAGTCACCAT CACCAGCTGC AGCCCTGCAC AGGGACCACA
    AGGGGGAGGG CCAGCTCCAC GAAAAGTAGG ACACTACACA GCTCAGGCTT GTATCAGGCT
    TCGTGGTTCT TGGGAGTGGA ATATAGAAGT TTCACGGTTT CTTCACAGTC AGAGATTATA
    CAAGCGATCC AGAATTTAAC CCGACTCCTC TATAGCCTTC AGGCCGCGCT GACCATCCAG
    GACAGCCACA TCCAGGTGCA CAGGCTGCTG CTGCAGCAGG CTGGCCTGCC GGCGTCCCAA
    GAGGAGCACG GCGAGCTGGC GAGGCAGCGG GAGGAGCTGG CCGCCCTGCC CGGGCTCCAG
    TGGCAGCTGC AGCAGGAGCG GCGGCGCGGC CAGCGCACCT GGGAGCGGCA GCAGCGCGAG
    CAGGAGGCCA GGGCCTGCCG GCTGCGGGAG CGCGAGCAGG GCTGCCGCGG CCGCGAGGAG
    CTGCTGCTCA GGGCCCGCGG GGAGCTGGAC CGGCAGCTGC GGGAGCACCG GCAGGACCTG
    GAGCGGCTGC GGGAGGGCCA GCGCCTGGTG GAGAGTGCGA GGGCCCGCAC GCGCGCCCAG
    CACAGCCTGC TGCGTGGCCC GAGGCCCGGG CGCCAGAGCA GCCTGCCCGC GGCCTTGCCT
    CCGGGCAGCG GCGAGGTGAT GGAACTTAAT CGATCCGAGA GCTTGTGTCA TGAAAATTCA
    TTCTTCATTA ATGAAGCTTT AGTACAAATG TCACTTAACA CTTACAACAA ATCAACTCCA
    TCAGGTGTCC ACCAGGAAGC CGCTTATCCC CAGAATATAG CTAATTCGGA CTTGGTAAGG
    ACTAGTGAAA GTCCCGTAGA CCTCCAGTTG GACATTTCTC AGCCCTTGGA TGTCAACCAC
    GAACTGTGGA CAGCCGCTGG GTCCTGTCAT CAGATACCCC CTCTCCACGA AAGCAGCAAG
    GATTCTTGTA AAAATGGCCT GCGCACCCCT CACCCGGGGC CCTCGGCCTC CCCGGAGCCC
    AGTGCACGCA GCTCCCGGCT GCAGACGGGG GCGGCAGAAG CAGCGCACAA CCTACAGACG
    CGGACCGGAC AAGATGCAGA GACCGGAGAC GCGGCTGAAG AAAATATCGT TTACCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013964535.1
    CDS92..5371
    Translation

    Target ORF information:

    RefSeq Version XM_014109060.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109060.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGAGTTGA GCTGCAGCGA AGTACCTCTT TATGGGCAGA TGACCGTCTA TGCAAAGTTT 
    GGGAAAAATG TTTATCTTCC TAAAGATGCC GAGTTTTACT TTATTTATAA TGGATCTCAT
    CAGAGACACG TCGTGATTGC AAAACGCACT GAGGATAATG TTCTCCAGTC CAGCATTCCA
    GGGCATGGGC TTCAGGAGAC GGTGACGGTG TCTGTATGCC TCTGCTCAGA AGGTTACTCC
    CCAGTGACCA TGGGCTCCGG CTCAGTGACC TACGTGGACA ACATGGCTTG CAGGCTGGCT
    CGTTTGCTGG TCACCCAGGC TGATCGCCTC ACAGCCAGTA GTCATCAGAC CTTTCTGACT
    CCATTTGCCC TGACGGTGGG AGCATTGCCT GCTTTGGACG AGGAGCTGGT GCTGGCTCTG
    ACCCATCTGG AGTTGCCTCT TGAGTGGAGT GTCTTGGGAA ATTCTTCACT TGAAGTTTCT
    TTATGCAGAG AGTCTCTTCT ACACCTGGCT ATGAGATGGG GCCTGCCTAA GCTCTCTCAT
    TTCCTCTTGT GCCTCCCTGG GGGAGTTCAG GCTTTGTCTT TACCCAATGA AGAAGGTGCC
    ACACCATTAG ACTTGGCTTT ACGTGCCGGA CACTCCAAGC TGGTTGAAGA CATCACAAAT
    TTCCAGGGCA GACGTTCCCC CGACCTCTCC CGAGTGCACC TCGGCGGACG TGCCTTCCTG
    CAGTATGTTC ACCCGTCGGG GACACTGACG CTGACGCTGA ACCACACAGC TGAGCATTTG
    TTGGAGGCAG ATATTAAACT CTTCAGGAAA TACTTTTTGG ACAGAGCCTT TCTCCTCAAG
    GCTCTTGACG ACCGAGAAGC TGGGCCAGAG GAGAGCCGGG GCATGCCCTC CCGGGCTGCC
    GCCACTGAGG AGGGTACGCG AGCTGCCTCC CCCGCGCCCA GGAACCCAGA AGAGATTAGG
    AATTTGGTGA CCGGCAGGCC ATCACCTGAA AAGGAGGATG TAAAGCGTGT GGACAGCCCG
    GACGTTGACC CCCATGAACA CGACGGCCGG GACAGTCTTG ATTTGGATCA CTCTTTCGAT
    GTCTTCAAAA AACCTAAGCA TCCCCCAGCG TTCTTTGCTG CAGGCGGGCT CTCTGATGTG
    CTGAATGGCG GCGATGAAGT TTACGCCAAC TGTATGGTGA TTGACCAGGT TGGTGATTTG
    GATATTAACT ACATCAATTT AGAAGGAATC TCTACAGACG CCTGTGCTGA ATCCATGGGT
    TCTACTCTGG GGCCTCAGAA CTGCGAGCAT ATCCTTGCTG CTGAAACCAG CCATGGGAAA
    TACCCACTAA GTGAGAACAA GGAAGTGGTC TCAGATACCG AAGCTCAGCA GTCCTTCCCA
    TCACCGTCTA GTTCATATGC TTCCAATTTT GATCTTTCCT TTGGTCTTCA TGGATTTGAG
    AAGGAACAAA GTCTCCTGAA GAAAAGAAGC TCCAGCCTGG ATGCCTTGGA TGCCGACAGC
    GAAGGGGAAG GGCATTCTGA GCGGTCACAC ATCTGCTACA CGCCAGCATC TCAGAGTTCC
    TCGAGGACTG GGATTCCCAG TGGGGATGAG CTGGACTCCT TCGAGACCGC CACCAAACCT
    GATTTTAGTA TCTCCAGGAC AGAGTCGCTT TCTTTATCAA GTAGCCTGCA GTTGAAGGAA
    TCATTGCTTC CTGGAGTCCG CTCACGTTCT TATTCCTGCT CATCACCCAA AATTTCTTTA
    GGAAAAACTC GATTGGTGCG TGATTTCACA GTGTGCAATT CTGGTGAAGA GCAAAGAGCC
    TTCGGCTTCC CGGAGCCCGC CAGGGAGAGG AGGATTCAAG AGGAAGAATG GGATAAATAC
    ATCATACCTG CCAAGTCAGA GTCTGAGAAA TACAAAGTGA GCCGGACCTT CAGCTTCCTC
    ATGAACAGGA TGACAAGCCC TCGGAACAAA TCTAAGACGA AAAGCAAGGA TGCCAAAGAC
    AAAGAGAAAC CGAGCCGACA TCAGTTTGTC CCCGGAACCT TCTCTGGGGT TCTCCAGTGT
    TCCGTTTGTG ATAAAACGCT CCTGGGGAAG GAGTCATCCC AGTGTTCCAA CTGTAATGCA
    AACGTCCACA AGGGTTGTAA GGACGCTGCA CCTCCGTGTA CCAAGAAATT CCAGGAGAAA
    TATAACAAGA ACAAACCGCA GACCATCCTT GGAAACTCTT CATTTCGAGA CATCCCGCAA
    CCCAGTCTCT CCTTGCACCC TTCTTCCTCC ATGCCTATCG GATTGCCCAC TGGGAGAAAA
    GAAGCTTCTG CCCAGGCCCA TCCCTTGTCC AGAAGTGTTC CAGGCACTGC CTTGGAAAGC
    TTCAGAAGAT CGGGGCTATC CCTGGAGTCT GAGAGTGACG GGAACAGCTG GAGAAGCAGG
    TCTCATTCCG ATGAGCTGTT ACAGTCCATG GGCTCTTCTC CCTCCACGGA ATCCTTCTTA
    ATGGAAGATG TCGTGGACTC TTCTCTATGG AGTGACCTCA GCAGTGACGC CCAGGAGTTG
    GAAGCAGAAT CCTGGAGTCT GGTGGTGGAC CCCTCCTTTT GCAATAAGCA GGAGAAAGAT
    GTCATCAAAA GACAGGATGT CATTTTCGAG CTTATGCAAA CAGAGATGCA TCATATCCAG
    ACCCTGCTCA TCATGTCTGA GATCTTCAGG AAAGGAATGA AGGAGGAGCT GCAGCTTGAT
    CACAGCACCG TGGATAAAAT CTTTCCCTGC TTAGATGAGT TACTTGAAAT CCACAGGCAT
    TTCTTTTATA GTATGAAGGA GCGAAGGCAG GAGTCCTGTG CTGGTGGTGA CAGGAATTTT
    GTCATCAGCC GAATTGGAGA TATTCTAGTA CAGCAGTTTT CAGAAGAAAA TGCAAATCAA
    ATGAAGAAAA TATATGGAGA ATTCTGTAGC CATCACAAAG AAGCTGTTAG CCTCTATAAA
    GAACTCCAAC AGAATAAGAA GTTTCAGAAT TTTATTAAGC TTCGAAATAG TAATCTTTTG
    GCTCGACGCC GAGGAATCCC AGAATGCATT CTGCTAGTCA CTCAGCGCAT CACAAAATAC
    CCTGTCCTGG TGGAACGGAT TTTGCAGTAC ACAAAGGAAA GAAGTGAAGA ACATCAAGAC
    TTGTGCAAAG CTCTGTGCTT AATTAAAGAC ATGATTGCAG CAGTGGATTT AAAAGTCAGC
    GAGTATGAGA AAAACCAAAA ATGGCTTGAG ATCCTAAATA AGATCGAAAA CAAAACCTAC
    ACAAAGCTCA AAAATGGCCA CGTGTTTAGG AAGCAAGCAC TGATAAGTAA AGAAAGGACT
    CTCTTATATG ATGGCCTTGT TTATTGGAAA ACTGCTACGG GTCGTTTCAA AGATATCCTG
    GCTCTACTTC TAACTGATGT GCTGCTCTTT TTACAAGAAA AAGACCAGAA ATACATCTTT
    GCAGCTGTTG ATCAGAAGCC ATCGGTTATT TCCCTTCAGA AGCTCATTGC TAGAGAAGTC
    GCCAACGAGG AAAGAGGAAT GTTCCTGATC AGTGCATCCT CTGCTGGTCC TGAGATGTAC
    GAAATTCACA CCAACTCCAA GGAGGAACGA AATAACTGGA TGAGACGAAT CCAACAGGCC
    GTGGAAAGTT GCCCAGAAGA AGAAGGGGGA AGGACAAGTG AATCTGATGA AGAGAGGCGG
    AAAGCTGAAG CAAGAGTGGC CAAAATCCAG CAATGTCAAG AAATACTCAG TAACCAAGAC
    CAACAGATTT GCGCATATTT GGAGGAGAAG CTGCATATCT ACGCTGAACT TGGAGAACTG
    AGTGGATTCA AGGATGTCCA TCTAGAGCCC CACCTCCTCA TCAAACCTGA CCCAGGGGAG
    CCTCCCCAGG CAGCCTCATT ACTAGCAGCA GCCCTGAGAG AAGCGGAGAG CCTGCAGGTT
    GCCGTGAAGG CCTCACAAGC GGGTGATGCT CGCCCATCAC CTGAGGAGGG TTCTGCAGAA
    CCTGCGGTTG CAGATGCACA GGGTCCTCCC GAGGAGCCAG GATCATCTCC AGCCTCACTA
    GTCACAGAAG GGACAGGAGG AAGAGGCTGT TGGGATGTGG ATCCTGGCCC CCAGGGTGTG
    GGAACCGACT TGGCGGTCTC TGATGCAGGG GAGAAGAGTC TTAAAAATCT GTGCTGCAGC
    CGGCAAAGTC CCTGGGGTGT CAGTCACCAT CACCAGCTGC AGCCCTGCAC AGGGACCACA
    AGGGGGAGGG CCAGCTCCAC GAAAAGTAGG ACACTACACA GCTCAGGCTT GTATCAGGCT
    TCGTGGTTCT TGGGAGTGGA ATATAGAAGT TTCACGGTTT CTTCACAGTC AGAGATTATA
    CAAGCGATCC AGAATTTAAC CCGACTCCTC TATAGCCTTC AGGCCGCGCT GACCATCCAG
    GACAGCCACA TCCAGGTGCA CAGGCTGCTG CTGCAGCAGG CTGGCCTGCC GGCGTCCCAA
    GAGGAGCACG GCGAGCTGGC GAGGCAGCGG GAGGAGCTGG CCGCCCTGCC CGGGCTCCAG
    TGGCAGCTGC AGCAGGAGCG GCGGCGCGGC CAGCGCACCT GGGAGCGGCA GCAGCGCGAG
    CAGGAGGCCA GGGCCTGCCG GCTGCGGGAG CGCGAGCAGG GCTGCCGCGG CCGCGAGGAG
    CTGCTGCTCA GGGCCCGCGG GGAGCTGGAC CGGCAGCTGC GGGAGCACCG GCAGGACCTG
    GAGCGGCTGC GGGAGGGCCA GCGCCTGGTG GAGAGTGCGA GGGCCCGCAC GCGCGCCCAG
    CACAGCCTGC TGCGTGGCCC GAGGCCCGGG CGCCAGAGCA GCCTGCCCGC GGCCTTGCCT
    CCGGGCAGCG GCGAGGTGAT GGAACTTAAT CGATCCGAGA GCTTGTGTCA TGAAAATTCA
    TTCTTCATTA ATGAAGCTTT AGTACAAATG TCACTTAACA CTTACAACAA ATCAACTCCA
    TCAGGTGTCC ACCAGGAAGC CGCTTATCCC CAGAATATAG CTAATTCGGA CTTGGTAAGG
    ACTAGTGAAA GTCCCGTAGA CCTCCAGTTG GACATTTCTC AGCCCTTGGA TGTCAACCAC
    GAACTGTGGA CAGCCGCTGG GTCCTGTCAT CAGATACCCC CTCTCCACGA AAGCAGCAAG
    GATTCTTGTA AAAATGGCCT GCGCACCCCT CACCCGGGGC CCTCGGCCTC CCCGGAGCCC
    AGTGCACGCA GCTCCCGGCT GCAGACGGGG GCGGCAGAAG CAGCGCACAA CCTACAGACG
    CGGACCGGAC AAGATGCAGA GACCGGAGAC GCGGCTGAAG AAAATATCGT TTACCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35382
    Clone ID Related Accession (Same CDS sequence) XM_014109061.1 , XM_014109061.2
    Accession Version XM_014109061.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5232bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product rho guanine nucleotide exchange factor 28 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGAGTTGA GCTGCAGCGA AGTACCTCTT TATGGGCAGA TGACCGTCTA TGCAAAGTTT 
    GGGAAAAATG TTTATCTTCC TAAAGATGCC GAGTTTTACT TTATTTATAA TGGATCTCAT
    CAGAGACACG TCGTGATTGC AAAACGCACT GAGGATAATG TTCTCCAGTC CAGCATTCCA
    GGGCATGGGC TTCAGGAGAC GGTGACGGTG TCTGTATGCC TCTGCTCAGA AGGTTACTCC
    CCAGTGACCA TGGGCTCCGG CTCAGTGACC TACGTGGACA ACATGGCTTG CAGGCTGGCT
    CGTTTGCTGG TCACCCAGGC TGATCGCCTC ACAGCCAGTA GTCATCAGAC CTTTCTGACT
    CCATTTGCCC TGACGGTGGG AGCATTGCCT GCTTTGGACG AGGAGCTGGT GCTGGCTCTG
    ACCCATCTGG AGTTGCCTCT TGAGTGGAGT GTCTTGGGAA ATTCTTCACT TGAAGTTTCT
    TTATGCAGAG AGTCTCTTCT ACACCTGGCT ATGAGATGGG GCCTGCCTAA GCTCTCTCAT
    TTCCTCTTGT GCCTCCCTGG GGGAGTTCAG GCTTTGTCTT TACCCAATGA AGAAGGTGCC
    ACACCATTAG ACTTGGCTTT ACGTGCCGGA CACTCCAAGC TGGTTGAAGA CATCACAAAT
    TTCCAGGGCA GACGTTCCCC CGACCTCTCC CGAGTGCACC TCGGCGGACG TGCCTTCCTG
    CAGTATGTTC ACCCGTCGGG GACACTGACG CTGACGCTGA ACCACACAGC TGAGCATTTG
    TTGGAGGCAG ATATTAAACT CTTCAGGAAA TACTTTTTGG ACAGAGCCTT TCTCCTCAAG
    GCTCTTGACG ACCGAGAAGC TGGGCCAGAG GAGAGCCGGG GCATGCCCTC CCGGGCTGCC
    GCCACTGAGG AGGGTACGCG AGCTGCCTCC CCCGCGCCCA GGAACCCAGA AGATCACTCT
    TTCGATGTCT TCAAAAAACC TAAGCATCCC CCAGCGTTCT TTGCTGCAGG CGGGCTCTCT
    GATGTGCTGA ATGGCGGCGA TGAAGTTTAC GCCAACTGTA TGGTGATTGA CCAGGTTGGT
    GATTTGGATA TTAACTACAT CAATTTAGAA GGAATCTCTA CAGACGCCTG TGCTGAATCC
    ATGGGTTCTA CTCTGGGGCC TCAGAACTGC GAGCATATCC TTGCTGCTGA AACCAGCCAT
    GGGAAATACC CACTAAGTGA GAACAAGGAA GTGGTCTCAG ATACCGAAGC TCAGCAGTCC
    TTCCCATCAC CGTCTAGTTC ATATGCTTCC AATTTTGATC TTTCCTTTGG TCTTCATGGA
    TTTGAGAAGG AACAAAGTCT CCTGAAGAAA AGAAGCTCCA GCCTGGATGC CTTGGATGCC
    GACAGCGAAG GGGAAGGGCA TTCTGAGCGG TCACACATCT GCTACACGCC AGCATCTCAG
    AGTTCCTCGA GGACTGGGAT TCCCAGTGGG GATGAGCTGG ACTCCTTCGA GACCGCCACC
    AAACCTGATT TTAGTATCTC CAGGACAGAG TCGCTTTCTT TATCAAGTAG CCTGCAGTTG
    AAGGAATCAT TGCTTCCTGG AGTCCGCTCA CGTTCTTATT CCTGCTCATC ACCCAAAATT
    TCTTTAGGAA AAACTCGATT GGTGCGTGAT TTCACAGTGT GCAATTCTGG TGAAGAGCAA
    AGAGCCTTCG GCTTCCCGGA GCCCGCCAGG GAGAGGAGGA TTCAAGAGGA AGAATGGGAT
    AAATACATCA TACCTGCCAA GTCAGAGTCT GAGAAATACA AAGTGAGCCG GACCTTCAGC
    TTCCTCATGA ACAGGATGAC AAGCCCTCGG AACAAATCTA AGACGAAAAG CAAGGATGCC
    AAAGACAAAG AGAAACCGAG CCGACATCAG TTTGTCCCCG GAACCTTCTC TGGGGTTCTC
    CAGTGTTCCG TTTGTGATAA AACGCTCCTG GGGAAGGAGT CATCCCAGTG TTCCAACTGT
    AATGCAAACG TCCACAAGGG TTGTAAGGAC GCTGCACCTC CGTGTACCAA GAAATTCCAG
    GAGAAATATA ACAAGAACAA ACCGCAGACC ATCCTTGGAA ACTCTTCATT TCGAGACATC
    CCGCAACCCA GTCTCTCCTT GCACCCTTCT TCCTCCATGC CTATCGGATT GCCCACTGGG
    AGAAAAGAAG CTTCTGCCCA GGCCCATCCC TTGTCCAGAA GTGTTCCAGG CACTGCCTTG
    GAAAGCTTCA GAAGATCGGG GCTATCCCTG GAGTCTGAGA GTGACGGGAA CAGCTGGAGA
    AGCAGGTCTC ATTCCGATGA GCTGTTACAG TCCATGGGCT CTTCTCCCTC CACGGAATCC
    TTCTTAATGG AAGATGTCGT GGACTCTTCT CTATGGAGTG ACCTCAGCAG TGACGCCCAG
    GAGTTGGAAG CAGAATCCTG GAGTCTGGTG GTGGACCCCT CCTTTTGCAA TAAGCAGGAG
    AAAGATGTCA TCAAAAGACA GGATGTCATT TTCGAGCTTA TGCAAACAGA GATGCATCAT
    ATCCAGACCC TGCTCATCAT GTCTGAGATC TTCAGGAAAG GAATGAAGGA GGAGCTGCAG
    CTTGATCACA GCACCGTGGA TAAAATCTTT CCCTGCTTAG ATGAGTTACT TGAAATCCAC
    AGGCATTTCT TTTATAGTAT GAAGGAGCGA AGGCAGGAGT CCTGTGCTGG TGGTGACAGG
    AATTTTGTCA TCAGCCGAAT TGGAGATATT CTAGTACAGC AGTTTTCAGA AGAAAATGCA
    AATCAAATGA AGAAAATATA TGGAGAATTC TGTAGCCATC ACAAAGAAGC TGTTAGCCTC
    TATAAAGAAC TCCAACAGAA TAAGAAGTTT CAGAATTTTA TTAAGCTTCG AAATAGTAAT
    CTTTTGGCTC GACGCCGAGG AATCCCAGAA TGCATTCTGC TAGTCACTCA GCGCATCACA
    AAATACCCTG TCCTGGTGGA ACGGATTTTG CAGTACACAA AGGAAAGAAG TGAAGAACAT
    CAAGACTTGT GCAAAGCTCT GTGCTTAATT AAAGACATGA TTGCAGCAGT GGATTTAAAA
    GTCAGCGAGT ATGAGAAAAA CCAAAAATGG CTTGAGATCC TAAATAAGAT CGAAAACAAA
    ACCTACACAA AGCTCAAAAA TGGCCACGTG TTTAGGAAGC AAGCACTGAT AAGTAAAGAA
    AGGACTCTCT TATATGATGG CCTTGTTTAT TGGAAAACTG CTACGGGTCG TTTCAAAGAT
    ATCCTGGCTC TACTTCTAAC TGATGTGCTG CTCTTTTTAC AAGAAAAAGA CCAGAAATAC
    ATCTTTGCAG CTGTTGATCA GAAGCCATCG GTTATTTCCC TTCAGAAGCT CATTGCTAGA
    GAAGTCGCCA ACGAGGAAAG AGGAATGTTC CTGATCAGTG CATCCTCTGC TGGTCCTGAG
    ATGTACGAAA TTCACACCAA CTCCAAGGAG GAACGAAATA ACTGGATGAG ACGAATCCAA
    CAGGCCGTGG AAAGTTGCCC AGAAGAAGAA GGGGGAAGGA CAAGTGAATC TGATGAAGAG
    AGGCGGAAAG CTGAAGCAAG AGTGGCCAAA ATCCAGCAAT GTCAAGAAAT ACTCAGTAAC
    CAAGACCAAC AGATTTGCGC ATATTTGGAG GAGAAGCTGC ATATCTACGC TGAACTTGGA
    GAACTGAGTG GATTCAAGGA TGTCCATCTA GAGCCCCACC TCCTCATCAA ACCTGACCCA
    GGGGAGCCTC CCCAGGCAGC CTCATTACTA GCAGCAGCCC TGAGAGAAGC GGAGAGCCTG
    CAGGTTGCCG TGAAGGCCTC ACAAGCGGGT GATGCTCGCC CATCACCTGA GGAGGGTTCT
    GCAGAACCTG CGGTTGCAGA TGCACAGGGT CCTCCCGAGG AGCCAGGATC ATCTCCAGCC
    TCACTAGTCA CAGAAGGGAC AGGAGGAAGA GGCTGTTGGG ATGTGGATCC TGGCCCCCAG
    GGTGTGGGAA CCGACTTGGC GGTCTCTGAT GCAGGGGAGA AGAGTCTTAA AAATCTGTGC
    TGCAGCCGGC AAAGTCCCTG GGGTGTCAGT CACCATCACC AGCTGCAGCC CTGCACAGGG
    ACCACAAGGG GGAGGGCCAG CTCCACGAAA AGTAGGACAC TACACAGCTC AGGCTTGTAT
    CAGGCTTCGT GGTTCTTGGG AGTGGAATAT AGAAGTTTCA CGGTTTCTTC ACAGTCAGAG
    ATTATACAAG CGATCCAGAA TTTAACCCGA CTCCTCTATA GCCTTCAGGC CGCGCTGACC
    ATCCAGGACA GCCACATCCA GGTGCACAGG CTGCTGCTGC AGCAGGCTGG CCTGCCGGCG
    TCCCAAGAGG AGCACGGCGA GCTGGCGAGG CAGCGGGAGG AGCTGGCCGC CCTGCCCGGG
    CTCCAGTGGC AGCTGCAGCA GGAGCGGCGG CGCGGCCAGC GCACCTGGGA GCGGCAGCAG
    CGCGAGCAGG AGGCCAGGGC CTGCCGGCTG CGGGAGCGCG AGCAGGGCTG CCGCGGCCGC
    GAGGAGCTGC TGCTCAGGGC CCGCGGGGAG CTGGACCGGC AGCTGCGGGA GCACCGGCAG
    GACCTGGAGC GGCTGCGGGA GGGCCAGCGC CTGGTGGAGA GTGCGAGGGC CCGCACGCGC
    GCCCAGCACA GCCTGCTGCG TGGCCCGAGG CCCGGGCGCC AGAGCAGCCT GCCCGCGGCC
    TTGCCTCCGG GCAGCGGCGA GGTGATGGAA CTTAATCGAT CCGAGAGCTT GTGTCATGAA
    AATTCATTCT TCATTAATGA AGCTTTAGTA CAAATGTCAC TTAACACTTA CAACAAATCA
    ACTCCATCAG GTGTCCACCA GGAAGCCGCT TATCCCCAGA ATATAGCTAA TTCGGACTTG
    GTAAGGACTA GTGAAAGTCC CGTAGACCTC CAGTTGGACA TTTCTCAGCC CTTGGATGTC
    AACCACGAAC TGTGGACAGC CGCTGGGTCC TGTCATCAGA TACCCCCTCT CCACGAAAGC
    AGCAAGGATT CTTGTAAAAA TGGAAGAATT TACATCAGTC AGCTATGTTT CCAGAGCCAT
    TCGTCAGAAA TGTTTTGGCG ACCAGACTTG ACCATCTCAG CTTGGGATAG AGAGCTTCCG
    AATCTGCAGA ACTTCCAACC GCTGTTGGTT GCATTATGTA GATGGTTGCG TTACGTCCGT
    ACATCCCCGG GAGATCTTGC CGTCATCCTC GAGTCCCCTG AGCAGGTTTT TCAGCTAGAA
    AGAAAATGTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013964536.1
    CDS12..5243
    Translation

    Target ORF information:

    RefSeq Version XM_014109061.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 28 (ARHGEF28), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109061.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGAGTTGA GCTGCAGCGA AGTACCTCTT TATGGGCAGA TGACCGTCTA TGCAAAGTTT 
    GGGAAAAATG TTTATCTTCC TAAAGATGCC GAGTTTTACT TTATTTATAA TGGATCTCAT
    CAGAGACACG TCGTGATTGC AAAACGCACT GAGGATAATG TTCTCCAGTC CAGCATTCCA
    GGGCATGGGC TTCAGGAGAC GGTGACGGTG TCTGTATGCC TCTGCTCAGA AGGTTACTCC
    CCAGTGACCA TGGGCTCCGG CTCAGTGACC TACGTGGACA ACATGGCTTG CAGGCTGGCT
    CGTTTGCTGG TCACCCAGGC TGATCGCCTC ACAGCCAGTA GTCATCAGAC CTTTCTGACT
    CCATTTGCCC TGACGGTGGG AGCATTGCCT GCTTTGGACG AGGAGCTGGT GCTGGCTCTG
    ACCCATCTGG AGTTGCCTCT TGAGTGGAGT GTCTTGGGAA ATTCTTCACT TGAAGTTTCT
    TTATGCAGAG AGTCTCTTCT ACACCTGGCT ATGAGATGGG GCCTGCCTAA GCTCTCTCAT
    TTCCTCTTGT GCCTCCCTGG GGGAGTTCAG GCTTTGTCTT TACCCAATGA AGAAGGTGCC
    ACACCATTAG ACTTGGCTTT ACGTGCCGGA CACTCCAAGC TGGTTGAAGA CATCACAAAT
    TTCCAGGGCA GACGTTCCCC CGACCTCTCC CGAGTGCACC TCGGCGGACG TGCCTTCCTG
    CAGTATGTTC ACCCGTCGGG GACACTGACG CTGACGCTGA ACCACACAGC TGAGCATTTG
    TTGGAGGCAG ATATTAAACT CTTCAGGAAA TACTTTTTGG ACAGAGCCTT TCTCCTCAAG
    GCTCTTGACG ACCGAGAAGC TGGGCCAGAG GAGAGCCGGG GCATGCCCTC CCGGGCTGCC
    GCCACTGAGG AGGGTACGCG AGCTGCCTCC CCCGCGCCCA GGAACCCAGA AGATCACTCT
    TTCGATGTCT TCAAAAAACC TAAGCATCCC CCAGCGTTCT TTGCTGCAGG CGGGCTCTCT
    GATGTGCTGA ATGGCGGCGA TGAAGTTTAC GCCAACTGTA TGGTGATTGA CCAGGTTGGT
    GATTTGGATA TTAACTACAT CAATTTAGAA GGAATCTCTA CAGACGCCTG TGCTGAATCC
    ATGGGTTCTA CTCTGGGGCC TCAGAACTGC GAGCATATCC TTGCTGCTGA AACCAGCCAT
    GGGAAATACC CACTAAGTGA GAACAAGGAA GTGGTCTCAG ATACCGAAGC TCAGCAGTCC
    TTCCCATCAC CGTCTAGTTC ATATGCTTCC AATTTTGATC TTTCCTTTGG TCTTCATGGA
    TTTGAGAAGG AACAAAGTCT CCTGAAGAAA AGAAGCTCCA GCCTGGATGC CTTGGATGCC
    GACAGCGAAG GGGAAGGGCA TTCTGAGCGG TCACACATCT GCTACACGCC AGCATCTCAG
    AGTTCCTCGA GGACTGGGAT TCCCAGTGGG GATGAGCTGG ACTCCTTCGA GACCGCCACC
    AAACCTGATT TTAGTATCTC CAGGACAGAG TCGCTTTCTT TATCAAGTAG CCTGCAGTTG
    AAGGAATCAT TGCTTCCTGG AGTCCGCTCA CGTTCTTATT CCTGCTCATC ACCCAAAATT
    TCTTTAGGAA AAACTCGATT GGTGCGTGAT TTCACAGTGT GCAATTCTGG TGAAGAGCAA
    AGAGCCTTCG GCTTCCCGGA GCCCGCCAGG GAGAGGAGGA TTCAAGAGGA AGAATGGGAT
    AAATACATCA TACCTGCCAA GTCAGAGTCT GAGAAATACA AAGTGAGCCG GACCTTCAGC
    TTCCTCATGA ACAGGATGAC AAGCCCTCGG AACAAATCTA AGACGAAAAG CAAGGATGCC
    AAAGACAAAG AGAAACCGAG CCGACATCAG TTTGTCCCCG GAACCTTCTC TGGGGTTCTC
    CAGTGTTCCG TTTGTGATAA AACGCTCCTG GGGAAGGAGT CATCCCAGTG TTCCAACTGT
    AATGCAAACG TCCACAAGGG TTGTAAGGAC GCTGCACCTC CGTGTACCAA GAAATTCCAG
    GAGAAATATA ACAAGAACAA ACCGCAGACC ATCCTTGGAA ACTCTTCATT TCGAGACATC
    CCGCAACCCA GTCTCTCCTT GCACCCTTCT TCCTCCATGC CTATCGGATT GCCCACTGGG
    AGAAAAGAAG CTTCTGCCCA GGCCCATCCC TTGTCCAGAA GTGTTCCAGG CACTGCCTTG
    GAAAGCTTCA GAAGATCGGG GCTATCCCTG GAGTCTGAGA GTGACGGGAA CAGCTGGAGA
    AGCAGGTCTC ATTCCGATGA GCTGTTACAG TCCATGGGCT CTTCTCCCTC CACGGAATCC
    TTCTTAATGG AAGATGTCGT GGACTCTTCT CTATGGAGTG ACCTCAGCAG TGACGCCCAG
    GAGTTGGAAG CAGAATCCTG GAGTCTGGTG GTGGACCCCT CCTTTTGCAA TAAGCAGGAG
    AAAGATGTCA TCAAAAGACA GGATGTCATT TTCGAGCTTA TGCAAACAGA GATGCATCAT
    ATCCAGACCC TGCTCATCAT GTCTGAGATC TTCAGGAAAG GAATGAAGGA GGAGCTGCAG
    CTTGATCACA GCACCGTGGA TAAAATCTTT CCCTGCTTAG ATGAGTTACT TGAAATCCAC
    AGGCATTTCT TTTATAGTAT GAAGGAGCGA AGGCAGGAGT CCTGTGCTGG TGGTGACAGG
    AATTTTGTCA TCAGCCGAAT TGGAGATATT CTAGTACAGC AGTTTTCAGA AGAAAATGCA
    AATCAAATGA AGAAAATATA TGGAGAATTC TGTAGCCATC ACAAAGAAGC TGTTAGCCTC
    TATAAAGAAC TCCAACAGAA TAAGAAGTTT CAGAATTTTA TTAAGCTTCG AAATAGTAAT
    CTTTTGGCTC GACGCCGAGG AATCCCAGAA TGCATTCTGC TAGTCACTCA GCGCATCACA
    AAATACCCTG TCCTGGTGGA ACGGATTTTG CAGTACACAA AGGAAAGAAG TGAAGAACAT
    CAAGACTTGT GCAAAGCTCT GTGCTTAATT AAAGACATGA TTGCAGCAGT GGATTTAAAA
    GTCAGCGAGT ATGAGAAAAA CCAAAAATGG CTTGAGATCC TAAATAAGAT CGAAAACAAA
    ACCTACACAA AGCTCAAAAA TGGCCACGTG TTTAGGAAGC AAGCACTGAT AAGTAAAGAA
    AGGACTCTCT TATATGATGG CCTTGTTTAT TGGAAAACTG CTACGGGTCG TTTCAAAGAT
    ATCCTGGCTC TACTTCTAAC TGATGTGCTG CTCTTTTTAC AAGAAAAAGA CCAGAAATAC
    ATCTTTGCAG CTGTTGATCA GAAGCCATCG GTTATTTCCC TTCAGAAGCT CATTGCTAGA
    GAAGTCGCCA ACGAGGAAAG AGGAATGTTC CTGATCAGTG CATCCTCTGC TGGTCCTGAG
    ATGTACGAAA TTCACACCAA CTCCAAGGAG GAACGAAATA ACTGGATGAG ACGAATCCAA
    CAGGCCGTGG AAAGTTGCCC AGAAGAAGAA GGGGGAAGGA CAAGTGAATC TGATGAAGAG
    AGGCGGAAAG CTGAAGCAAG AGTGGCCAAA ATCCAGCAAT GTCAAGAAAT ACTCAGTAAC
    CAAGACCAAC AGATTTGCGC ATATTTGGAG GAGAAGCTGC ATATCTACGC TGAACTTGGA
    GAACTGAGTG GATTCAAGGA TGTCCATCTA GAGCCCCACC TCCTCATCAA ACCTGACCCA
    GGGGAGCCTC CCCAGGCAGC CTCATTACTA GCAGCAGCCC TGAGAGAAGC GGAGAGCCTG
    CAGGTTGCCG TGAAGGCCTC ACAAGCGGGT GATGCTCGCC CATCACCTGA GGAGGGTTCT
    GCAGAACCTG CGGTTGCAGA TGCACAGGGT CCTCCCGAGG AGCCAGGATC ATCTCCAGCC
    TCACTAGTCA CAGAAGGGAC AGGAGGAAGA GGCTGTTGGG ATGTGGATCC TGGCCCCCAG
    GGTGTGGGAA CCGACTTGGC GGTCTCTGAT GCAGGGGAGA AGAGTCTTAA AAATCTGTGC
    TGCAGCCGGC AAAGTCCCTG GGGTGTCAGT CACCATCACC AGCTGCAGCC CTGCACAGGG
    ACCACAAGGG GGAGGGCCAG CTCCACGAAA AGTAGGACAC TACACAGCTC AGGCTTGTAT
    CAGGCTTCGT GGTTCTTGGG AGTGGAATAT AGAAGTTTCA CGGTTTCTTC ACAGTCAGAG
    ATTATACAAG CGATCCAGAA TTTAACCCGA CTCCTCTATA GCCTTCAGGC CGCGCTGACC
    ATCCAGGACA GCCACATCCA GGTGCACAGG CTGCTGCTGC AGCAGGCTGG CCTGCCGGCG
    TCCCAAGAGG AGCACGGCGA GCTGGCGAGG CAGCGGGAGG AGCTGGCCGC CCTGCCCGGG
    CTCCAGTGGC AGCTGCAGCA GGAGCGGCGG CGCGGCCAGC GCACCTGGGA GCGGCAGCAG
    CGCGAGCAGG AGGCCAGGGC CTGCCGGCTG CGGGAGCGCG AGCAGGGCTG CCGCGGCCGC
    GAGGAGCTGC TGCTCAGGGC CCGCGGGGAG CTGGACCGGC AGCTGCGGGA GCACCGGCAG
    GACCTGGAGC GGCTGCGGGA GGGCCAGCGC CTGGTGGAGA GTGCGAGGGC CCGCACGCGC
    GCCCAGCACA GCCTGCTGCG TGGCCCGAGG CCCGGGCGCC AGAGCAGCCT GCCCGCGGCC
    TTGCCTCCGG GCAGCGGCGA GGTGATGGAA CTTAATCGAT CCGAGAGCTT GTGTCATGAA
    AATTCATTCT TCATTAATGA AGCTTTAGTA CAAATGTCAC TTAACACTTA CAACAAATCA
    ACTCCATCAG GTGTCCACCA GGAAGCCGCT TATCCCCAGA ATATAGCTAA TTCGGACTTG
    GTAAGGACTA GTGAAAGTCC CGTAGACCTC CAGTTGGACA TTTCTCAGCC CTTGGATGTC
    AACCACGAAC TGTGGACAGC CGCTGGGTCC TGTCATCAGA TACCCCCTCT CCACGAAAGC
    AGCAAGGATT CTTGTAAAAA TGGAAGAATT TACATCAGTC AGCTATGTTT CCAGAGCCAT
    TCGTCAGAAA TGTTTTGGCG ACCAGACTTG ACCATCTCAG CTTGGGATAG AGAGCTTCCG
    AATCTGCAGA ACTTCCAACC GCTGTTGGTT GCATTATGTA GATGGTTGCG TTACGTCCGT
    ACATCCCCGG GAGATCTTGC CGTCATCCTC GAGTCCCCTG AGCAGGTTTT TCAGCTAGAA
    AGAAAATGTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35382
    Clone ID Related Accession (Same CDS sequence) XM_014109061.1 , XM_014109061.2
    Accession Version XM_014109061.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5232bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rho guanine nucleotide exchange factor 28 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109061.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGAGTTGA GCTGCAGCGA AGTACCTCTT TATGGGCAGA TGACCGTCTA TGCAAAGTTT 
    GGGAAAAATG TTTATCTTCC TAAAGATGCC GAGTTTTACT TTATTTATAA TGGATCTCAT
    CAGAGACACG TCGTGATTGC AAAACGCACT GAGGATAATG TTCTCCAGTC CAGCATTCCA
    GGGCATGGGC TTCAGGAGAC GGTGACGGTG TCTGTATGCC TCTGCTCAGA AGGTTACTCC
    CCAGTGACCA TGGGCTCCGG CTCAGTGACC TACGTGGACA ACATGGCTTG CAGGCTGGCT
    CGTTTGCTGG TCACCCAGGC TGATCGCCTC ACAGCCAGTA GTCATCAGAC CTTTCTGACT
    CCATTTGCCC TGACGGTGGG AGCATTGCCT GCTTTGGACG AGGAGCTGGT GCTGGCTCTG
    ACCCATCTGG AGTTGCCTCT TGAGTGGAGT GTCTTGGGAA ATTCTTCACT TGAAGTTTCT
    TTATGCAGAG AGTCTCTTCT ACACCTGGCT ATGAGATGGG GCCTGCCTAA GCTCTCTCAT
    TTCCTCTTGT GCCTCCCTGG GGGAGTTCAG GCTTTGTCTT TACCCAATGA AGAAGGTGCC
    ACACCATTAG ACTTGGCTTT ACGTGCCGGA CACTCCAAGC TGGTTGAAGA CATCACAAAT
    TTCCAGGGCA GACGTTCCCC CGACCTCTCC CGAGTGCACC TCGGCGGACG TGCCTTCCTG
    CAGTATGTTC ACCCGTCGGG GACACTGACG CTGACGCTGA ACCACACAGC TGAGCATTTG
    TTGGAGGCAG ATATTAAACT CTTCAGGAAA TACTTTTTGG ACAGAGCCTT TCTCCTCAAG
    GCTCTTGACG ACCGAGAAGC TGGGCCAGAG GAGAGCCGGG GCATGCCCTC CCGGGCTGCC
    GCCACTGAGG AGGGTACGCG AGCTGCCTCC CCCGCGCCCA GGAACCCAGA AGATCACTCT
    TTCGATGTCT TCAAAAAACC TAAGCATCCC CCAGCGTTCT TTGCTGCAGG CGGGCTCTCT
    GATGTGCTGA ATGGCGGCGA TGAAGTTTAC GCCAACTGTA TGGTGATTGA CCAGGTTGGT
    GATTTGGATA TTAACTACAT CAATTTAGAA GGAATCTCTA CAGACGCCTG TGCTGAATCC
    ATGGGTTCTA CTCTGGGGCC TCAGAACTGC GAGCATATCC TTGCTGCTGA AACCAGCCAT
    GGGAAATACC CACTAAGTGA GAACAAGGAA GTGGTCTCAG ATACCGAAGC TCAGCAGTCC
    TTCCCATCAC CGTCTAGTTC ATATGCTTCC AATTTTGATC TTTCCTTTGG TCTTCATGGA
    TTTGAGAAGG AACAAAGTCT CCTGAAGAAA AGAAGCTCCA GCCTGGATGC CTTGGATGCC
    GACAGCGAAG GGGAAGGGCA TTCTGAGCGG TCACACATCT GCTACACGCC AGCATCTCAG
    AGTTCCTCGA GGACTGGGAT TCCCAGTGGG GATGAGCTGG ACTCCTTCGA GACCGCCACC
    AAACCTGATT TTAGTATCTC CAGGACAGAG TCGCTTTCTT TATCAAGTAG CCTGCAGTTG
    AAGGAATCAT TGCTTCCTGG AGTCCGCTCA CGTTCTTATT CCTGCTCATC ACCCAAAATT
    TCTTTAGGAA AAACTCGATT GGTGCGTGAT TTCACAGTGT GCAATTCTGG TGAAGAGCAA
    AGAGCCTTCG GCTTCCCGGA GCCCGCCAGG GAGAGGAGGA TTCAAGAGGA AGAATGGGAT
    AAATACATCA TACCTGCCAA GTCAGAGTCT GAGAAATACA AAGTGAGCCG GACCTTCAGC
    TTCCTCATGA ACAGGATGAC AAGCCCTCGG AACAAATCTA AGACGAAAAG CAAGGATGCC
    AAAGACAAAG AGAAACCGAG CCGACATCAG TTTGTCCCCG GAACCTTCTC TGGGGTTCTC
    CAGTGTTCCG TTTGTGATAA AACGCTCCTG GGGAAGGAGT CATCCCAGTG TTCCAACTGT
    AATGCAAACG TCCACAAGGG TTGTAAGGAC GCTGCACCTC CGTGTACCAA GAAATTCCAG
    GAGAAATATA ACAAGAACAA ACCGCAGACC ATCCTTGGAA ACTCTTCATT TCGAGACATC
    CCGCAACCCA GTCTCTCCTT GCACCCTTCT TCCTCCATGC CTATCGGATT GCCCACTGGG
    AGAAAAGAAG CTTCTGCCCA GGCCCATCCC TTGTCCAGAA GTGTTCCAGG CACTGCCTTG
    GAAAGCTTCA GAAGATCGGG GCTATCCCTG GAGTCTGAGA GTGACGGGAA CAGCTGGAGA
    AGCAGGTCTC ATTCCGATGA GCTGTTACAG TCCATGGGCT CTTCTCCCTC CACGGAATCC
    TTCTTAATGG AAGATGTCGT GGACTCTTCT CTATGGAGTG ACCTCAGCAG TGACGCCCAG
    GAGTTGGAAG CAGAATCCTG GAGTCTGGTG GTGGACCCCT CCTTTTGCAA TAAGCAGGAG
    AAAGATGTCA TCAAAAGACA GGATGTCATT TTCGAGCTTA TGCAAACAGA GATGCATCAT
    ATCCAGACCC TGCTCATCAT GTCTGAGATC TTCAGGAAAG GAATGAAGGA GGAGCTGCAG
    CTTGATCACA GCACCGTGGA TAAAATCTTT CCCTGCTTAG ATGAGTTACT TGAAATCCAC
    AGGCATTTCT TTTATAGTAT GAAGGAGCGA AGGCAGGAGT CCTGTGCTGG TGGTGACAGG
    AATTTTGTCA TCAGCCGAAT TGGAGATATT CTAGTACAGC AGTTTTCAGA AGAAAATGCA
    AATCAAATGA AGAAAATATA TGGAGAATTC TGTAGCCATC ACAAAGAAGC TGTTAGCCTC
    TATAAAGAAC TCCAACAGAA TAAGAAGTTT CAGAATTTTA TTAAGCTTCG AAATAGTAAT
    CTTTTGGCTC GACGCCGAGG AATCCCAGAA TGCATTCTGC TAGTCACTCA GCGCATCACA
    AAATACCCTG TCCTGGTGGA ACGGATTTTG CAGTACACAA AGGAAAGAAG TGAAGAACAT
    CAAGACTTGT GCAAAGCTCT GTGCTTAATT AAAGACATGA TTGCAGCAGT GGATTTAAAA
    GTCAGCGAGT ATGAGAAAAA CCAAAAATGG CTTGAGATCC TAAATAAGAT CGAAAACAAA
    ACCTACACAA AGCTCAAAAA TGGCCACGTG TTTAGGAAGC AAGCACTGAT AAGTAAAGAA
    AGGACTCTCT TATATGATGG CCTTGTTTAT TGGAAAACTG CTACGGGTCG TTTCAAAGAT
    ATCCTGGCTC TACTTCTAAC TGATGTGCTG CTCTTTTTAC AAGAAAAAGA CCAGAAATAC
    ATCTTTGCAG CTGTTGATCA GAAGCCATCG GTTATTTCCC TTCAGAAGCT CATTGCTAGA
    GAAGTCGCCA ACGAGGAAAG AGGAATGTTC CTGATCAGTG CATCCTCTGC TGGTCCTGAG
    ATGTACGAAA TTCACACCAA CTCCAAGGAG GAACGAAATA ACTGGATGAG ACGAATCCAA
    CAGGCCGTGG AAAGTTGCCC AGAAGAAGAA GGGGGAAGGA CAAGTGAATC TGATGAAGAG
    AGGCGGAAAG CTGAAGCAAG AGTGGCCAAA ATCCAGCAAT GTCAAGAAAT ACTCAGTAAC
    CAAGACCAAC AGATTTGCGC ATATTTGGAG GAGAAGCTGC ATATCTACGC TGAACTTGGA
    GAACTGAGTG GATTCAAGGA TGTCCATCTA GAGCCCCACC TCCTCATCAA ACCTGACCCA
    GGGGAGCCTC CCCAGGCAGC CTCATTACTA GCAGCAGCCC TGAGAGAAGC GGAGAGCCTG
    CAGGTTGCCG TGAAGGCCTC ACAAGCGGGT GATGCTCGCC CATCACCTGA GGAGGGTTCT
    GCAGAACCTG CGGTTGCAGA TGCACAGGGT CCTCCCGAGG AGCCAGGATC ATCTCCAGCC
    TCACTAGTCA CAGAAGGGAC AGGAGGAAGA GGCTGTTGGG ATGTGGATCC TGGCCCCCAG
    GGTGTGGGAA CCGACTTGGC GGTCTCTGAT GCAGGGGAGA AGAGTCTTAA AAATCTGTGC
    TGCAGCCGGC AAAGTCCCTG GGGTGTCAGT CACCATCACC AGCTGCAGCC CTGCACAGGG
    ACCACAAGGG GGAGGGCCAG CTCCACGAAA AGTAGGACAC TACACAGCTC AGGCTTGTAT
    CAGGCTTCGT GGTTCTTGGG AGTGGAATAT AGAAGTTTCA CGGTTTCTTC ACAGTCAGAG
    ATTATACAAG CGATCCAGAA TTTAACCCGA CTCCTCTATA GCCTTCAGGC CGCGCTGACC
    ATCCAGGACA GCCACATCCA GGTGCACAGG CTGCTGCTGC AGCAGGCTGG CCTGCCGGCG
    TCCCAAGAGG AGCACGGCGA GCTGGCGAGG CAGCGGGAGG AGCTGGCCGC CCTGCCCGGG
    CTCCAGTGGC AGCTGCAGCA GGAGCGGCGG CGCGGCCAGC GCACCTGGGA GCGGCAGCAG
    CGCGAGCAGG AGGCCAGGGC CTGCCGGCTG CGGGAGCGCG AGCAGGGCTG CCGCGGCCGC
    GAGGAGCTGC TGCTCAGGGC CCGCGGGGAG CTGGACCGGC AGCTGCGGGA GCACCGGCAG
    GACCTGGAGC GGCTGCGGGA GGGCCAGCGC CTGGTGGAGA GTGCGAGGGC CCGCACGCGC
    GCCCAGCACA GCCTGCTGCG TGGCCCGAGG CCCGGGCGCC AGAGCAGCCT GCCCGCGGCC
    TTGCCTCCGG GCAGCGGCGA GGTGATGGAA CTTAATCGAT CCGAGAGCTT GTGTCATGAA
    AATTCATTCT TCATTAATGA AGCTTTAGTA CAAATGTCAC TTAACACTTA CAACAAATCA
    ACTCCATCAG GTGTCCACCA GGAAGCCGCT TATCCCCAGA ATATAGCTAA TTCGGACTTG
    GTAAGGACTA GTGAAAGTCC CGTAGACCTC CAGTTGGACA TTTCTCAGCC CTTGGATGTC
    AACCACGAAC TGTGGACAGC CGCTGGGTCC TGTCATCAGA TACCCCCTCT CCACGAAAGC
    AGCAAGGATT CTTGTAAAAA TGGAAGAATT TACATCAGTC AGCTATGTTT CCAGAGCCAT
    TCGTCAGAAA TGTTTTGGCG ACCAGACTTG ACCATCTCAG CTTGGGATAG AGAGCTTCCG
    AATCTGCAGA ACTTCCAACC GCTGTTGGTT GCATTATGTA GATGGTTGCG TTACGTCCGT
    ACATCCCCGG GAGATCTTGC CGTCATCCTC GAGTCCCCTG AGCAGGTTTT TCAGCTAGAA
    AGAAAATGTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013964536.1
    CDS87..5318
    Translation

    Target ORF information:

    RefSeq Version XM_014109061.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109061.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGAGTTGA GCTGCAGCGA AGTACCTCTT TATGGGCAGA TGACCGTCTA TGCAAAGTTT 
    GGGAAAAATG TTTATCTTCC TAAAGATGCC GAGTTTTACT TTATTTATAA TGGATCTCAT
    CAGAGACACG TCGTGATTGC AAAACGCACT GAGGATAATG TTCTCCAGTC CAGCATTCCA
    GGGCATGGGC TTCAGGAGAC GGTGACGGTG TCTGTATGCC TCTGCTCAGA AGGTTACTCC
    CCAGTGACCA TGGGCTCCGG CTCAGTGACC TACGTGGACA ACATGGCTTG CAGGCTGGCT
    CGTTTGCTGG TCACCCAGGC TGATCGCCTC ACAGCCAGTA GTCATCAGAC CTTTCTGACT
    CCATTTGCCC TGACGGTGGG AGCATTGCCT GCTTTGGACG AGGAGCTGGT GCTGGCTCTG
    ACCCATCTGG AGTTGCCTCT TGAGTGGAGT GTCTTGGGAA ATTCTTCACT TGAAGTTTCT
    TTATGCAGAG AGTCTCTTCT ACACCTGGCT ATGAGATGGG GCCTGCCTAA GCTCTCTCAT
    TTCCTCTTGT GCCTCCCTGG GGGAGTTCAG GCTTTGTCTT TACCCAATGA AGAAGGTGCC
    ACACCATTAG ACTTGGCTTT ACGTGCCGGA CACTCCAAGC TGGTTGAAGA CATCACAAAT
    TTCCAGGGCA GACGTTCCCC CGACCTCTCC CGAGTGCACC TCGGCGGACG TGCCTTCCTG
    CAGTATGTTC ACCCGTCGGG GACACTGACG CTGACGCTGA ACCACACAGC TGAGCATTTG
    TTGGAGGCAG ATATTAAACT CTTCAGGAAA TACTTTTTGG ACAGAGCCTT TCTCCTCAAG
    GCTCTTGACG ACCGAGAAGC TGGGCCAGAG GAGAGCCGGG GCATGCCCTC CCGGGCTGCC
    GCCACTGAGG AGGGTACGCG AGCTGCCTCC CCCGCGCCCA GGAACCCAGA AGATCACTCT
    TTCGATGTCT TCAAAAAACC TAAGCATCCC CCAGCGTTCT TTGCTGCAGG CGGGCTCTCT
    GATGTGCTGA ATGGCGGCGA TGAAGTTTAC GCCAACTGTA TGGTGATTGA CCAGGTTGGT
    GATTTGGATA TTAACTACAT CAATTTAGAA GGAATCTCTA CAGACGCCTG TGCTGAATCC
    ATGGGTTCTA CTCTGGGGCC TCAGAACTGC GAGCATATCC TTGCTGCTGA AACCAGCCAT
    GGGAAATACC CACTAAGTGA GAACAAGGAA GTGGTCTCAG ATACCGAAGC TCAGCAGTCC
    TTCCCATCAC CGTCTAGTTC ATATGCTTCC AATTTTGATC TTTCCTTTGG TCTTCATGGA
    TTTGAGAAGG AACAAAGTCT CCTGAAGAAA AGAAGCTCCA GCCTGGATGC CTTGGATGCC
    GACAGCGAAG GGGAAGGGCA TTCTGAGCGG TCACACATCT GCTACACGCC AGCATCTCAG
    AGTTCCTCGA GGACTGGGAT TCCCAGTGGG GATGAGCTGG ACTCCTTCGA GACCGCCACC
    AAACCTGATT TTAGTATCTC CAGGACAGAG TCGCTTTCTT TATCAAGTAG CCTGCAGTTG
    AAGGAATCAT TGCTTCCTGG AGTCCGCTCA CGTTCTTATT CCTGCTCATC ACCCAAAATT
    TCTTTAGGAA AAACTCGATT GGTGCGTGAT TTCACAGTGT GCAATTCTGG TGAAGAGCAA
    AGAGCCTTCG GCTTCCCGGA GCCCGCCAGG GAGAGGAGGA TTCAAGAGGA AGAATGGGAT
    AAATACATCA TACCTGCCAA GTCAGAGTCT GAGAAATACA AAGTGAGCCG GACCTTCAGC
    TTCCTCATGA ACAGGATGAC AAGCCCTCGG AACAAATCTA AGACGAAAAG CAAGGATGCC
    AAAGACAAAG AGAAACCGAG CCGACATCAG TTTGTCCCCG GAACCTTCTC TGGGGTTCTC
    CAGTGTTCCG TTTGTGATAA AACGCTCCTG GGGAAGGAGT CATCCCAGTG TTCCAACTGT
    AATGCAAACG TCCACAAGGG TTGTAAGGAC GCTGCACCTC CGTGTACCAA GAAATTCCAG
    GAGAAATATA ACAAGAACAA ACCGCAGACC ATCCTTGGAA ACTCTTCATT TCGAGACATC
    CCGCAACCCA GTCTCTCCTT GCACCCTTCT TCCTCCATGC CTATCGGATT GCCCACTGGG
    AGAAAAGAAG CTTCTGCCCA GGCCCATCCC TTGTCCAGAA GTGTTCCAGG CACTGCCTTG
    GAAAGCTTCA GAAGATCGGG GCTATCCCTG GAGTCTGAGA GTGACGGGAA CAGCTGGAGA
    AGCAGGTCTC ATTCCGATGA GCTGTTACAG TCCATGGGCT CTTCTCCCTC CACGGAATCC
    TTCTTAATGG AAGATGTCGT GGACTCTTCT CTATGGAGTG ACCTCAGCAG TGACGCCCAG
    GAGTTGGAAG CAGAATCCTG GAGTCTGGTG GTGGACCCCT CCTTTTGCAA TAAGCAGGAG
    AAAGATGTCA TCAAAAGACA GGATGTCATT TTCGAGCTTA TGCAAACAGA GATGCATCAT
    ATCCAGACCC TGCTCATCAT GTCTGAGATC TTCAGGAAAG GAATGAAGGA GGAGCTGCAG
    CTTGATCACA GCACCGTGGA TAAAATCTTT CCCTGCTTAG ATGAGTTACT TGAAATCCAC
    AGGCATTTCT TTTATAGTAT GAAGGAGCGA AGGCAGGAGT CCTGTGCTGG TGGTGACAGG
    AATTTTGTCA TCAGCCGAAT TGGAGATATT CTAGTACAGC AGTTTTCAGA AGAAAATGCA
    AATCAAATGA AGAAAATATA TGGAGAATTC TGTAGCCATC ACAAAGAAGC TGTTAGCCTC
    TATAAAGAAC TCCAACAGAA TAAGAAGTTT CAGAATTTTA TTAAGCTTCG AAATAGTAAT
    CTTTTGGCTC GACGCCGAGG AATCCCAGAA TGCATTCTGC TAGTCACTCA GCGCATCACA
    AAATACCCTG TCCTGGTGGA ACGGATTTTG CAGTACACAA AGGAAAGAAG TGAAGAACAT
    CAAGACTTGT GCAAAGCTCT GTGCTTAATT AAAGACATGA TTGCAGCAGT GGATTTAAAA
    GTCAGCGAGT ATGAGAAAAA CCAAAAATGG CTTGAGATCC TAAATAAGAT CGAAAACAAA
    ACCTACACAA AGCTCAAAAA TGGCCACGTG TTTAGGAAGC AAGCACTGAT AAGTAAAGAA
    AGGACTCTCT TATATGATGG CCTTGTTTAT TGGAAAACTG CTACGGGTCG TTTCAAAGAT
    ATCCTGGCTC TACTTCTAAC TGATGTGCTG CTCTTTTTAC AAGAAAAAGA CCAGAAATAC
    ATCTTTGCAG CTGTTGATCA GAAGCCATCG GTTATTTCCC TTCAGAAGCT CATTGCTAGA
    GAAGTCGCCA ACGAGGAAAG AGGAATGTTC CTGATCAGTG CATCCTCTGC TGGTCCTGAG
    ATGTACGAAA TTCACACCAA CTCCAAGGAG GAACGAAATA ACTGGATGAG ACGAATCCAA
    CAGGCCGTGG AAAGTTGCCC AGAAGAAGAA GGGGGAAGGA CAAGTGAATC TGATGAAGAG
    AGGCGGAAAG CTGAAGCAAG AGTGGCCAAA ATCCAGCAAT GTCAAGAAAT ACTCAGTAAC
    CAAGACCAAC AGATTTGCGC ATATTTGGAG GAGAAGCTGC ATATCTACGC TGAACTTGGA
    GAACTGAGTG GATTCAAGGA TGTCCATCTA GAGCCCCACC TCCTCATCAA ACCTGACCCA
    GGGGAGCCTC CCCAGGCAGC CTCATTACTA GCAGCAGCCC TGAGAGAAGC GGAGAGCCTG
    CAGGTTGCCG TGAAGGCCTC ACAAGCGGGT GATGCTCGCC CATCACCTGA GGAGGGTTCT
    GCAGAACCTG CGGTTGCAGA TGCACAGGGT CCTCCCGAGG AGCCAGGATC ATCTCCAGCC
    TCACTAGTCA CAGAAGGGAC AGGAGGAAGA GGCTGTTGGG ATGTGGATCC TGGCCCCCAG
    GGTGTGGGAA CCGACTTGGC GGTCTCTGAT GCAGGGGAGA AGAGTCTTAA AAATCTGTGC
    TGCAGCCGGC AAAGTCCCTG GGGTGTCAGT CACCATCACC AGCTGCAGCC CTGCACAGGG
    ACCACAAGGG GGAGGGCCAG CTCCACGAAA AGTAGGACAC TACACAGCTC AGGCTTGTAT
    CAGGCTTCGT GGTTCTTGGG AGTGGAATAT AGAAGTTTCA CGGTTTCTTC ACAGTCAGAG
    ATTATACAAG CGATCCAGAA TTTAACCCGA CTCCTCTATA GCCTTCAGGC CGCGCTGACC
    ATCCAGGACA GCCACATCCA GGTGCACAGG CTGCTGCTGC AGCAGGCTGG CCTGCCGGCG
    TCCCAAGAGG AGCACGGCGA GCTGGCGAGG CAGCGGGAGG AGCTGGCCGC CCTGCCCGGG
    CTCCAGTGGC AGCTGCAGCA GGAGCGGCGG CGCGGCCAGC GCACCTGGGA GCGGCAGCAG
    CGCGAGCAGG AGGCCAGGGC CTGCCGGCTG CGGGAGCGCG AGCAGGGCTG CCGCGGCCGC
    GAGGAGCTGC TGCTCAGGGC CCGCGGGGAG CTGGACCGGC AGCTGCGGGA GCACCGGCAG
    GACCTGGAGC GGCTGCGGGA GGGCCAGCGC CTGGTGGAGA GTGCGAGGGC CCGCACGCGC
    GCCCAGCACA GCCTGCTGCG TGGCCCGAGG CCCGGGCGCC AGAGCAGCCT GCCCGCGGCC
    TTGCCTCCGG GCAGCGGCGA GGTGATGGAA CTTAATCGAT CCGAGAGCTT GTGTCATGAA
    AATTCATTCT TCATTAATGA AGCTTTAGTA CAAATGTCAC TTAACACTTA CAACAAATCA
    ACTCCATCAG GTGTCCACCA GGAAGCCGCT TATCCCCAGA ATATAGCTAA TTCGGACTTG
    GTAAGGACTA GTGAAAGTCC CGTAGACCTC CAGTTGGACA TTTCTCAGCC CTTGGATGTC
    AACCACGAAC TGTGGACAGC CGCTGGGTCC TGTCATCAGA TACCCCCTCT CCACGAAAGC
    AGCAAGGATT CTTGTAAAAA TGGAAGAATT TACATCAGTC AGCTATGTTT CCAGAGCCAT
    TCGTCAGAAA TGTTTTGGCG ACCAGACTTG ACCATCTCAG CTTGGGATAG AGAGCTTCCG
    AATCTGCAGA ACTTCCAACC GCTGTTGGTT GCATTATGTA GATGGTTGCG TTACGTCCGT
    ACATCCCCGG GAGATCTTGC CGTCATCCTC GAGTCCCCTG AGCAGGTTTT TCAGCTAGAA
    AGAAAATGTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35383
    Clone ID Related Accession (Same CDS sequence) XM_014109065.1 , XM_014109065.2
    Accession Version XM_014109065.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5187bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product rho guanine nucleotide exchange factor 28 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGAGTTGA GCTGCAGCGA AGTACCTCTT TATGGGCAGA TGACCGTCTA TGCAAAGTTT 
    GGGAAAAATG TTTATCTTCC TAAAGATGCC GAGTTTTACT TTATTTATAA TGGATCTCAT
    CAGAGACACG TCGTGATTGC AAAACGCACT GAGGATAATG TTCTCCAGTC CAGCATTCCA
    GGGCATGGGC TTCAGGAGAC GGTGACGGTG TCTGTATGCC TCTGCTCAGA AGGTTACTCC
    CCAGTGACCA TGGGCTCCGG CTCAGTGACC TACGTGGACA ACATGGCTTG CAGGCTGGCT
    CGTTTGCTGG TCACCCAGGC TGATCGCCTC ACAGCCAGTA GTCATCAGAC CTTTCTGACT
    CCATTTGCCC TGACGGTGGG AGCATTGCCT GCTTTGGACG AGGAGCTGGT GCTGGCTCTG
    ACCCATCTGG AGTTGCCTCT TGAGTGGAGT GTCTTGGGAA ATTCTTCACT TGAAGTTTCT
    TTATGCAGAG AGTCTCTTCT ACACCTGGCT ATGAGATGGG GCCTGCCTAA GCTCTCTCAT
    TTCCTCTTGT GCCTCCCTGG GGGAGTTCAG GCTTTGTCTT TACCCAATGA AGAAGGTGCC
    ACACCATTAG ACTTGGCTTT ACGTGCCGGA CACTCCAAGC TGGTTGAAGA CATCACAAAT
    TTCCAGGGCA GACGTTCCCC CGACCTCTCC CGAGTGCACC TCGGCGGACG TGCCTTCCTG
    CAGTATGTTC ACCCGTCGGG GACACTGACG CTGACGCTGA ACCACACAGC TGAGCATTTG
    TTGGAGGCAG ATATTAAACT CTTCAGGAAA TACTTTTTGG ACAGAGCCTT TCTCCTCAAG
    GCTCTTGACG ACCGAGAAGC TGGGCCAGAG GAGAGCCGGG GCATGCCCTC CCGGGCTGCC
    GCCACTGAGG AGGGTACGCG AGCTGCCTCC CCCGCGCCCA GGAACCCAGA AGAGATTAGG
    AATTTGGTGA CCGGCAGGCC ATCACCTGAA AAGGAGGATG TAAAGCGTGT GGACAGCCCG
    GACGTTGACC CCCATGAACA CGACGGCCGG GACAGTCTTG ATTTGGATCA CTCTTTCGAT
    GTCTTCAAAA AACCTAAGCA TCCCCCAGCG TTCTTTGCTG CAGGCGGGCT CTCTGATGTG
    CTGAATGGCG GCGATGAAGT TTACGCCAAC TGTATGGTGA TTGACCAGGT TGGTGATTTG
    GATATTAACT ACATCAATTT AGAAGGAATC TCTACAGACG CCTGTGCTGA ATCCATGGGT
    TCTACTCTGG GGCCTCAGAA CTGCGAGCAT ATCCTTGCTG CTGAAACCAG CCATGGGAAA
    TACCCACTAA GTGAGAACAA GGAAGTGGTC TCAGATACCG AAGCTCAGCA GTCCTTCCCA
    TCACCGTCTA GTTCATATGC TTCCAATTTT GATCTTTCCT TTGGTCTTCA TGGATTTGAG
    AAGGAACAAA GTCTCCTGAA GAAAAGAAGC TCCAGCCTGG ATGCCTTGGA TGCCGACAGC
    GAAGGGGAAG GGCATTCTGA GCGGTCACAC ATCTGCTACA CGCCAGCATC TCAGAGTTCC
    TCGAGGACTG GGATTCCCAG TGGGGATGAG CTGGACTCCT TCGAGACCGC CACCAAACCT
    GATTTTAGTA TCTCCAGGAC AGAGTCGCTT TCTTTATCAA GTAGCCTGCA GTTGAAGGAA
    TCATTGCTTC CTGGAGTCCG CTCACGTTCT TATTCCTGCT CATCACCCAA AATTTCTTTA
    GGAAAAACTC GATTGGTGCG TGATTTCACA GTGTGCAATT CTGGTGAAGA GCAAAGAGCC
    TTCGGCTTCC CGGAGCCCGC CAGGGAGAGG AGGATTCAAG AGGAAGAATG GGATAAATAC
    ATCATACCTG CCAAGTCAGA GTCTGAGAAA TACAAAGTGA GCCGGACCTT CAGCTTCCTC
    ATGAACAGGA TGACAAGCCC TCGGAACAAA TCTAAGACGA AAAGCAAGGA TGCCAAAGAC
    AAAGAGAAAC CGAGCCGACA TCAGTTTGTC CCCGGAACCT TCTCTGGGGT TCTCCAGTGT
    TCCGTTTGTG ATAAAACGCT CCTGGGGAAG GAGTCATCCC AGTGTTCCAA CTGTAATGCA
    AACGTCCACA AGGGTTGTAA GGACGCTGCA CCTCCGTGTA CCAAGAAATT CCAGGAGAAA
    TATAACAAGA ACAAACCGCA GACCATCCTT GGAAACTCTT CATTTCGAGA CATCCCGCAA
    CCCAGTCTCT CCTTGCACCC TTCTTCCTCC ATGCCTATCG GATTGCCCAC TGGGAGAAAA
    GAAGCTTCTG CCCAGGCCCA TCCCTTGTCC AGAAGTGTTC CAGGCACTGC CTTGGAAAGC
    TTCAGAAGAT CGGGGCTATC CCTGGAGTCT GAGAGTGACG GGAACAGCTG GAGAAGCAGG
    TCTCATTCCG ATGAGCTGTT ACAGTCCATG GGCTCTTCTC CCTCCACGGA ATCCTTCTTA
    ATGGAAGATG TCGTGGACTC TTCTCTATGG AGTGACCTCA GCAGTGACGC CCAGGAGTTG
    GAAGCAGAAT CCTGGAGTCT GGTGGTGGAC CCCTCCTTTT GCAATAAGCA GGAGAAAGAT
    GTCATCAAAA GACAGGATGT CATTTTCGAG CTTATGCAAA CAGAGATGCA TCATATCCAG
    ACCCTGCTCA TCATGTCTGA GATCTTCAGG AAAGGAATGA AGGAGGAGCT GCAGCTTGAT
    CACAGCACCG TGGATAAAAT CTTTCCCTGC TTAGATGAGT TACTTGAAAT CCACAGGCAT
    TTCTTTTATA GTATGAAGGA GCGAAGGCAG GAGTCCTGTG CTGGTGGTGA CAGGAATTTT
    GTCATCAGCC GAATTGGAGA TATTCTAGTA CAGCAGTTTT CAGAAGAAAA TGCAAATCAA
    ATGAAGAAAA TATATGGAGA ATTCTGTAGC CATCACAAAG AAGCTGTTAG CCTCTATAAA
    GAACTCCAAC AGAATAAGAA GTTTCAGAAT TTTATTAAGC TTCGAAATAG TAATCTTTTG
    GCTCGACGCC GAGGAATCCC AGAATGCATT CTGCTAGTCA CTCAGCGCAT CACAAAATAC
    CCTGTCCTGG TGGAACGGAT TTTGCAGTAC ACAAAGGAAA GAAGTGAAGA ACATCAAGAC
    TTGTGCAAAG CTCTGTGCTT AATTAAAGAC ATGATTGCAG CAGTGGATTT AAAAGTCAGC
    GAGTATGAGA AAAACCAAAA ATGGCTTGAG ATCCTAAATA AGATCGAAAA CAAAACCTAC
    ACAAAGCTCA AAAATGGCCA CGTGTTTAGG AAGCAAGCAC TGATAAGTAA AGAAAGGACT
    CTCTTATATG ATGGCCTTGT TTATTGGAAA ACTGCTACGG GTCGTTTCAA AGATATCCTG
    GCTCTACTTC TAACTGATGT GCTGCTCTTT TTACAAGAAA AAGACCAGAA ATACATCTTT
    GCAGCTGTTG ATCAGAAGCC ATCGGTTATT TCCCTTCAGA AGCTCATTGC TAGAGAAGTC
    GCCAACGAGG AAAGAGGAAT GTTCCTGATC AGTGCATCCT CTGCTGGTCC TGAGATGTAC
    GAAATTCACA CCAACTCCAA GGAGGAACGA AATAACTGGA TGAGACGAAT CCAACAGGCC
    GTGGAAAGTT GCCCAGAAGA AGAAGGGGGA AGGACAAGTG AATCTGATGA AGAGAGGCGG
    AAAGCTGAAG CAAGAGTGGC CAAAATCCAG CAATGTCAAG AAATACTCAG TAACCAAGAC
    CAACAGATTT GCGCATATTT GGAGGAGAAG CTGCATATCT ACGCTGAACT TGGAGAACTG
    AGTGGATTCA AGGATGTCCA TCTAGAGCCC CACCTCCTCA TCAAACCTGA CCCAGGGGAG
    CCTCCCCAGG CAGCCTCATT ACTAGCAGCA GCCCTGAGAG AAGCGGAGAG CCTGCAGGTT
    GCCGTGAAGG CCTCACAAGC GGGTGATGCT CGCCCATCAC CTGAGGAGGG TTCTGCAGAA
    CCTGCGGTTG CAGATGCACA GGGTCCTCCC GAGGAGCCAG GATCATCTCC AGCCTCACTA
    GTCACAGAAG GGACAGGAGG AAGAGGCTGT TGGGATGTGG ATCCTGGCCC CCAGGGTGTG
    GGAACCGACT TGGCGGTCTC TGATGCAGGG GAGAAGGTGG AATATAGAAG TTTCACGGTT
    TCTTCACAGT CAGAGATTAT ACAAGCGATC CAGAATTTAA CCCGACTCCT CTATAGCCTT
    CAGGCCGCGC TGACCATCCA GGACAGCCAC ATCCAGGTGC ACAGGCTGCT GCTGCAGCAG
    GCTGGCCTGC CGGCGTCCCA AGAGGAGCAC GGCGAGCTGG CGAGGCAGCG GGAGGAGCTG
    GCCGCCCTGC CCGGGCTCCA GTGGCAGCTG CAGCAGGAGC GGCGGCGCGG CCAGCGCACC
    TGGGAGCGGC AGCAGCGCGA GCAGGAGGCC AGGGCCTGCC GGCTGCGGGA GCGCGAGCAG
    GGCTGCCGCG GCCGCGAGGA GCTGCTGCTC AGGGCCCGCG GGGAGCTGGA CCGGCAGCTG
    CGGGAGCACC GGCAGGACCT GGAGCGGCTG CGGGAGGGCC AGCGCCTGGT GGAGAGTGCG
    AGGGCCCGCA CGCGCGCCCA GCACAGCCTG CTGCGTGGCC CGAGGCCCGG GCGCCAGAGC
    AGCCTGCCCG CGGCCTTGCC TCCGGGCAGC GGCGAGGTGA TGGAACTTAA TCGATCCGAG
    AGCTTGTGTC ATGAAAATTC ATTCTTCATT AATGAAGCTT TAGTACAAAT GTCACTTAAC
    ACTTACAACA AATCAACTCC ATCAGGTGTC CACCAGGAAG CCGCTTATCC CCAGAATATA
    GCTAATTCGG ACTTGGTAAG GACTAGTGAA AGTCCCGTAG ACCTCCAGTT GGACATTTCT
    CAGCCCTTGG ATGTCAACCA CGAACTGTGG ACAGCCGCTG GGTCCTGTCA TCAGATACCC
    CCTCTCCACG AAAGCAGCAA GGATTCTTGT AAAAATGGAA GAATTTACAT CAGTCAGCTA
    TGTTTCCAGA GCCATTCGTC AGAAATGTTT TGGCGACCAG ACTTGACCAT CTCAGCTTGG
    GATAGAGAGC TTCCGAATCT GCAGAACTTC CAACCGCTGT TGGTTGCATT ATGTAGATGG
    TTGCGTTACG TCCGTACATC CCCGGGAGAT CTTGCCGTCA TCCTCGAGTC CCCTGAGCAG
    GTTTTTCAGC TAGAAAGAAA ATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013964540.1
    CDS12..5198
    Translation

    Target ORF information:

    RefSeq Version XM_014109065.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 28 (ARHGEF28), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109065.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGAGTTGA GCTGCAGCGA AGTACCTCTT TATGGGCAGA TGACCGTCTA TGCAAAGTTT 
    GGGAAAAATG TTTATCTTCC TAAAGATGCC GAGTTTTACT TTATTTATAA TGGATCTCAT
    CAGAGACACG TCGTGATTGC AAAACGCACT GAGGATAATG TTCTCCAGTC CAGCATTCCA
    GGGCATGGGC TTCAGGAGAC GGTGACGGTG TCTGTATGCC TCTGCTCAGA AGGTTACTCC
    CCAGTGACCA TGGGCTCCGG CTCAGTGACC TACGTGGACA ACATGGCTTG CAGGCTGGCT
    CGTTTGCTGG TCACCCAGGC TGATCGCCTC ACAGCCAGTA GTCATCAGAC CTTTCTGACT
    CCATTTGCCC TGACGGTGGG AGCATTGCCT GCTTTGGACG AGGAGCTGGT GCTGGCTCTG
    ACCCATCTGG AGTTGCCTCT TGAGTGGAGT GTCTTGGGAA ATTCTTCACT TGAAGTTTCT
    TTATGCAGAG AGTCTCTTCT ACACCTGGCT ATGAGATGGG GCCTGCCTAA GCTCTCTCAT
    TTCCTCTTGT GCCTCCCTGG GGGAGTTCAG GCTTTGTCTT TACCCAATGA AGAAGGTGCC
    ACACCATTAG ACTTGGCTTT ACGTGCCGGA CACTCCAAGC TGGTTGAAGA CATCACAAAT
    TTCCAGGGCA GACGTTCCCC CGACCTCTCC CGAGTGCACC TCGGCGGACG TGCCTTCCTG
    CAGTATGTTC ACCCGTCGGG GACACTGACG CTGACGCTGA ACCACACAGC TGAGCATTTG
    TTGGAGGCAG ATATTAAACT CTTCAGGAAA TACTTTTTGG ACAGAGCCTT TCTCCTCAAG
    GCTCTTGACG ACCGAGAAGC TGGGCCAGAG GAGAGCCGGG GCATGCCCTC CCGGGCTGCC
    GCCACTGAGG AGGGTACGCG AGCTGCCTCC CCCGCGCCCA GGAACCCAGA AGAGATTAGG
    AATTTGGTGA CCGGCAGGCC ATCACCTGAA AAGGAGGATG TAAAGCGTGT GGACAGCCCG
    GACGTTGACC CCCATGAACA CGACGGCCGG GACAGTCTTG ATTTGGATCA CTCTTTCGAT
    GTCTTCAAAA AACCTAAGCA TCCCCCAGCG TTCTTTGCTG CAGGCGGGCT CTCTGATGTG
    CTGAATGGCG GCGATGAAGT TTACGCCAAC TGTATGGTGA TTGACCAGGT TGGTGATTTG
    GATATTAACT ACATCAATTT AGAAGGAATC TCTACAGACG CCTGTGCTGA ATCCATGGGT
    TCTACTCTGG GGCCTCAGAA CTGCGAGCAT ATCCTTGCTG CTGAAACCAG CCATGGGAAA
    TACCCACTAA GTGAGAACAA GGAAGTGGTC TCAGATACCG AAGCTCAGCA GTCCTTCCCA
    TCACCGTCTA GTTCATATGC TTCCAATTTT GATCTTTCCT TTGGTCTTCA TGGATTTGAG
    AAGGAACAAA GTCTCCTGAA GAAAAGAAGC TCCAGCCTGG ATGCCTTGGA TGCCGACAGC
    GAAGGGGAAG GGCATTCTGA GCGGTCACAC ATCTGCTACA CGCCAGCATC TCAGAGTTCC
    TCGAGGACTG GGATTCCCAG TGGGGATGAG CTGGACTCCT TCGAGACCGC CACCAAACCT
    GATTTTAGTA TCTCCAGGAC AGAGTCGCTT TCTTTATCAA GTAGCCTGCA GTTGAAGGAA
    TCATTGCTTC CTGGAGTCCG CTCACGTTCT TATTCCTGCT CATCACCCAA AATTTCTTTA
    GGAAAAACTC GATTGGTGCG TGATTTCACA GTGTGCAATT CTGGTGAAGA GCAAAGAGCC
    TTCGGCTTCC CGGAGCCCGC CAGGGAGAGG AGGATTCAAG AGGAAGAATG GGATAAATAC
    ATCATACCTG CCAAGTCAGA GTCTGAGAAA TACAAAGTGA GCCGGACCTT CAGCTTCCTC
    ATGAACAGGA TGACAAGCCC TCGGAACAAA TCTAAGACGA AAAGCAAGGA TGCCAAAGAC
    AAAGAGAAAC CGAGCCGACA TCAGTTTGTC CCCGGAACCT TCTCTGGGGT TCTCCAGTGT
    TCCGTTTGTG ATAAAACGCT CCTGGGGAAG GAGTCATCCC AGTGTTCCAA CTGTAATGCA
    AACGTCCACA AGGGTTGTAA GGACGCTGCA CCTCCGTGTA CCAAGAAATT CCAGGAGAAA
    TATAACAAGA ACAAACCGCA GACCATCCTT GGAAACTCTT CATTTCGAGA CATCCCGCAA
    CCCAGTCTCT CCTTGCACCC TTCTTCCTCC ATGCCTATCG GATTGCCCAC TGGGAGAAAA
    GAAGCTTCTG CCCAGGCCCA TCCCTTGTCC AGAAGTGTTC CAGGCACTGC CTTGGAAAGC
    TTCAGAAGAT CGGGGCTATC CCTGGAGTCT GAGAGTGACG GGAACAGCTG GAGAAGCAGG
    TCTCATTCCG ATGAGCTGTT ACAGTCCATG GGCTCTTCTC CCTCCACGGA ATCCTTCTTA
    ATGGAAGATG TCGTGGACTC TTCTCTATGG AGTGACCTCA GCAGTGACGC CCAGGAGTTG
    GAAGCAGAAT CCTGGAGTCT GGTGGTGGAC CCCTCCTTTT GCAATAAGCA GGAGAAAGAT
    GTCATCAAAA GACAGGATGT CATTTTCGAG CTTATGCAAA CAGAGATGCA TCATATCCAG
    ACCCTGCTCA TCATGTCTGA GATCTTCAGG AAAGGAATGA AGGAGGAGCT GCAGCTTGAT
    CACAGCACCG TGGATAAAAT CTTTCCCTGC TTAGATGAGT TACTTGAAAT CCACAGGCAT
    TTCTTTTATA GTATGAAGGA GCGAAGGCAG GAGTCCTGTG CTGGTGGTGA CAGGAATTTT
    GTCATCAGCC GAATTGGAGA TATTCTAGTA CAGCAGTTTT CAGAAGAAAA TGCAAATCAA
    ATGAAGAAAA TATATGGAGA ATTCTGTAGC CATCACAAAG AAGCTGTTAG CCTCTATAAA
    GAACTCCAAC AGAATAAGAA GTTTCAGAAT TTTATTAAGC TTCGAAATAG TAATCTTTTG
    GCTCGACGCC GAGGAATCCC AGAATGCATT CTGCTAGTCA CTCAGCGCAT CACAAAATAC
    CCTGTCCTGG TGGAACGGAT TTTGCAGTAC ACAAAGGAAA GAAGTGAAGA ACATCAAGAC
    TTGTGCAAAG CTCTGTGCTT AATTAAAGAC ATGATTGCAG CAGTGGATTT AAAAGTCAGC
    GAGTATGAGA AAAACCAAAA ATGGCTTGAG ATCCTAAATA AGATCGAAAA CAAAACCTAC
    ACAAAGCTCA AAAATGGCCA CGTGTTTAGG AAGCAAGCAC TGATAAGTAA AGAAAGGACT
    CTCTTATATG ATGGCCTTGT TTATTGGAAA ACTGCTACGG GTCGTTTCAA AGATATCCTG
    GCTCTACTTC TAACTGATGT GCTGCTCTTT TTACAAGAAA AAGACCAGAA ATACATCTTT
    GCAGCTGTTG ATCAGAAGCC ATCGGTTATT TCCCTTCAGA AGCTCATTGC TAGAGAAGTC
    GCCAACGAGG AAAGAGGAAT GTTCCTGATC AGTGCATCCT CTGCTGGTCC TGAGATGTAC
    GAAATTCACA CCAACTCCAA GGAGGAACGA AATAACTGGA TGAGACGAAT CCAACAGGCC
    GTGGAAAGTT GCCCAGAAGA AGAAGGGGGA AGGACAAGTG AATCTGATGA AGAGAGGCGG
    AAAGCTGAAG CAAGAGTGGC CAAAATCCAG CAATGTCAAG AAATACTCAG TAACCAAGAC
    CAACAGATTT GCGCATATTT GGAGGAGAAG CTGCATATCT ACGCTGAACT TGGAGAACTG
    AGTGGATTCA AGGATGTCCA TCTAGAGCCC CACCTCCTCA TCAAACCTGA CCCAGGGGAG
    CCTCCCCAGG CAGCCTCATT ACTAGCAGCA GCCCTGAGAG AAGCGGAGAG CCTGCAGGTT
    GCCGTGAAGG CCTCACAAGC GGGTGATGCT CGCCCATCAC CTGAGGAGGG TTCTGCAGAA
    CCTGCGGTTG CAGATGCACA GGGTCCTCCC GAGGAGCCAG GATCATCTCC AGCCTCACTA
    GTCACAGAAG GGACAGGAGG AAGAGGCTGT TGGGATGTGG ATCCTGGCCC CCAGGGTGTG
    GGAACCGACT TGGCGGTCTC TGATGCAGGG GAGAAGGTGG AATATAGAAG TTTCACGGTT
    TCTTCACAGT CAGAGATTAT ACAAGCGATC CAGAATTTAA CCCGACTCCT CTATAGCCTT
    CAGGCCGCGC TGACCATCCA GGACAGCCAC ATCCAGGTGC ACAGGCTGCT GCTGCAGCAG
    GCTGGCCTGC CGGCGTCCCA AGAGGAGCAC GGCGAGCTGG CGAGGCAGCG GGAGGAGCTG
    GCCGCCCTGC CCGGGCTCCA GTGGCAGCTG CAGCAGGAGC GGCGGCGCGG CCAGCGCACC
    TGGGAGCGGC AGCAGCGCGA GCAGGAGGCC AGGGCCTGCC GGCTGCGGGA GCGCGAGCAG
    GGCTGCCGCG GCCGCGAGGA GCTGCTGCTC AGGGCCCGCG GGGAGCTGGA CCGGCAGCTG
    CGGGAGCACC GGCAGGACCT GGAGCGGCTG CGGGAGGGCC AGCGCCTGGT GGAGAGTGCG
    AGGGCCCGCA CGCGCGCCCA GCACAGCCTG CTGCGTGGCC CGAGGCCCGG GCGCCAGAGC
    AGCCTGCCCG CGGCCTTGCC TCCGGGCAGC GGCGAGGTGA TGGAACTTAA TCGATCCGAG
    AGCTTGTGTC ATGAAAATTC ATTCTTCATT AATGAAGCTT TAGTACAAAT GTCACTTAAC
    ACTTACAACA AATCAACTCC ATCAGGTGTC CACCAGGAAG CCGCTTATCC CCAGAATATA
    GCTAATTCGG ACTTGGTAAG GACTAGTGAA AGTCCCGTAG ACCTCCAGTT GGACATTTCT
    CAGCCCTTGG ATGTCAACCA CGAACTGTGG ACAGCCGCTG GGTCCTGTCA TCAGATACCC
    CCTCTCCACG AAAGCAGCAA GGATTCTTGT AAAAATGGAA GAATTTACAT CAGTCAGCTA
    TGTTTCCAGA GCCATTCGTC AGAAATGTTT TGGCGACCAG ACTTGACCAT CTCAGCTTGG
    GATAGAGAGC TTCCGAATCT GCAGAACTTC CAACCGCTGT TGGTTGCATT ATGTAGATGG
    TTGCGTTACG TCCGTACATC CCCGGGAGAT CTTGCCGTCA TCCTCGAGTC CCCTGAGCAG
    GTTTTTCAGC TAGAAAGAAA ATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35383
    Clone ID Related Accession (Same CDS sequence) XM_014109065.1 , XM_014109065.2
    Accession Version XM_014109065.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5187bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rho guanine nucleotide exchange factor 28 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109065.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGAGTTGA GCTGCAGCGA AGTACCTCTT TATGGGCAGA TGACCGTCTA TGCAAAGTTT 
    GGGAAAAATG TTTATCTTCC TAAAGATGCC GAGTTTTACT TTATTTATAA TGGATCTCAT
    CAGAGACACG TCGTGATTGC AAAACGCACT GAGGATAATG TTCTCCAGTC CAGCATTCCA
    GGGCATGGGC TTCAGGAGAC GGTGACGGTG TCTGTATGCC TCTGCTCAGA AGGTTACTCC
    CCAGTGACCA TGGGCTCCGG CTCAGTGACC TACGTGGACA ACATGGCTTG CAGGCTGGCT
    CGTTTGCTGG TCACCCAGGC TGATCGCCTC ACAGCCAGTA GTCATCAGAC CTTTCTGACT
    CCATTTGCCC TGACGGTGGG AGCATTGCCT GCTTTGGACG AGGAGCTGGT GCTGGCTCTG
    ACCCATCTGG AGTTGCCTCT TGAGTGGAGT GTCTTGGGAA ATTCTTCACT TGAAGTTTCT
    TTATGCAGAG AGTCTCTTCT ACACCTGGCT ATGAGATGGG GCCTGCCTAA GCTCTCTCAT
    TTCCTCTTGT GCCTCCCTGG GGGAGTTCAG GCTTTGTCTT TACCCAATGA AGAAGGTGCC
    ACACCATTAG ACTTGGCTTT ACGTGCCGGA CACTCCAAGC TGGTTGAAGA CATCACAAAT
    TTCCAGGGCA GACGTTCCCC CGACCTCTCC CGAGTGCACC TCGGCGGACG TGCCTTCCTG
    CAGTATGTTC ACCCGTCGGG GACACTGACG CTGACGCTGA ACCACACAGC TGAGCATTTG
    TTGGAGGCAG ATATTAAACT CTTCAGGAAA TACTTTTTGG ACAGAGCCTT TCTCCTCAAG
    GCTCTTGACG ACCGAGAAGC TGGGCCAGAG GAGAGCCGGG GCATGCCCTC CCGGGCTGCC
    GCCACTGAGG AGGGTACGCG AGCTGCCTCC CCCGCGCCCA GGAACCCAGA AGAGATTAGG
    AATTTGGTGA CCGGCAGGCC ATCACCTGAA AAGGAGGATG TAAAGCGTGT GGACAGCCCG
    GACGTTGACC CCCATGAACA CGACGGCCGG GACAGTCTTG ATTTGGATCA CTCTTTCGAT
    GTCTTCAAAA AACCTAAGCA TCCCCCAGCG TTCTTTGCTG CAGGCGGGCT CTCTGATGTG
    CTGAATGGCG GCGATGAAGT TTACGCCAAC TGTATGGTGA TTGACCAGGT TGGTGATTTG
    GATATTAACT ACATCAATTT AGAAGGAATC TCTACAGACG CCTGTGCTGA ATCCATGGGT
    TCTACTCTGG GGCCTCAGAA CTGCGAGCAT ATCCTTGCTG CTGAAACCAG CCATGGGAAA
    TACCCACTAA GTGAGAACAA GGAAGTGGTC TCAGATACCG AAGCTCAGCA GTCCTTCCCA
    TCACCGTCTA GTTCATATGC TTCCAATTTT GATCTTTCCT TTGGTCTTCA TGGATTTGAG
    AAGGAACAAA GTCTCCTGAA GAAAAGAAGC TCCAGCCTGG ATGCCTTGGA TGCCGACAGC
    GAAGGGGAAG GGCATTCTGA GCGGTCACAC ATCTGCTACA CGCCAGCATC TCAGAGTTCC
    TCGAGGACTG GGATTCCCAG TGGGGATGAG CTGGACTCCT TCGAGACCGC CACCAAACCT
    GATTTTAGTA TCTCCAGGAC AGAGTCGCTT TCTTTATCAA GTAGCCTGCA GTTGAAGGAA
    TCATTGCTTC CTGGAGTCCG CTCACGTTCT TATTCCTGCT CATCACCCAA AATTTCTTTA
    GGAAAAACTC GATTGGTGCG TGATTTCACA GTGTGCAATT CTGGTGAAGA GCAAAGAGCC
    TTCGGCTTCC CGGAGCCCGC CAGGGAGAGG AGGATTCAAG AGGAAGAATG GGATAAATAC
    ATCATACCTG CCAAGTCAGA GTCTGAGAAA TACAAAGTGA GCCGGACCTT CAGCTTCCTC
    ATGAACAGGA TGACAAGCCC TCGGAACAAA TCTAAGACGA AAAGCAAGGA TGCCAAAGAC
    AAAGAGAAAC CGAGCCGACA TCAGTTTGTC CCCGGAACCT TCTCTGGGGT TCTCCAGTGT
    TCCGTTTGTG ATAAAACGCT CCTGGGGAAG GAGTCATCCC AGTGTTCCAA CTGTAATGCA
    AACGTCCACA AGGGTTGTAA GGACGCTGCA CCTCCGTGTA CCAAGAAATT CCAGGAGAAA
    TATAACAAGA ACAAACCGCA GACCATCCTT GGAAACTCTT CATTTCGAGA CATCCCGCAA
    CCCAGTCTCT CCTTGCACCC TTCTTCCTCC ATGCCTATCG GATTGCCCAC TGGGAGAAAA
    GAAGCTTCTG CCCAGGCCCA TCCCTTGTCC AGAAGTGTTC CAGGCACTGC CTTGGAAAGC
    TTCAGAAGAT CGGGGCTATC CCTGGAGTCT GAGAGTGACG GGAACAGCTG GAGAAGCAGG
    TCTCATTCCG ATGAGCTGTT ACAGTCCATG GGCTCTTCTC CCTCCACGGA ATCCTTCTTA
    ATGGAAGATG TCGTGGACTC TTCTCTATGG AGTGACCTCA GCAGTGACGC CCAGGAGTTG
    GAAGCAGAAT CCTGGAGTCT GGTGGTGGAC CCCTCCTTTT GCAATAAGCA GGAGAAAGAT
    GTCATCAAAA GACAGGATGT CATTTTCGAG CTTATGCAAA CAGAGATGCA TCATATCCAG
    ACCCTGCTCA TCATGTCTGA GATCTTCAGG AAAGGAATGA AGGAGGAGCT GCAGCTTGAT
    CACAGCACCG TGGATAAAAT CTTTCCCTGC TTAGATGAGT TACTTGAAAT CCACAGGCAT
    TTCTTTTATA GTATGAAGGA GCGAAGGCAG GAGTCCTGTG CTGGTGGTGA CAGGAATTTT
    GTCATCAGCC GAATTGGAGA TATTCTAGTA CAGCAGTTTT CAGAAGAAAA TGCAAATCAA
    ATGAAGAAAA TATATGGAGA ATTCTGTAGC CATCACAAAG AAGCTGTTAG CCTCTATAAA
    GAACTCCAAC AGAATAAGAA GTTTCAGAAT TTTATTAAGC TTCGAAATAG TAATCTTTTG
    GCTCGACGCC GAGGAATCCC AGAATGCATT CTGCTAGTCA CTCAGCGCAT CACAAAATAC
    CCTGTCCTGG TGGAACGGAT TTTGCAGTAC ACAAAGGAAA GAAGTGAAGA ACATCAAGAC
    TTGTGCAAAG CTCTGTGCTT AATTAAAGAC ATGATTGCAG CAGTGGATTT AAAAGTCAGC
    GAGTATGAGA AAAACCAAAA ATGGCTTGAG ATCCTAAATA AGATCGAAAA CAAAACCTAC
    ACAAAGCTCA AAAATGGCCA CGTGTTTAGG AAGCAAGCAC TGATAAGTAA AGAAAGGACT
    CTCTTATATG ATGGCCTTGT TTATTGGAAA ACTGCTACGG GTCGTTTCAA AGATATCCTG
    GCTCTACTTC TAACTGATGT GCTGCTCTTT TTACAAGAAA AAGACCAGAA ATACATCTTT
    GCAGCTGTTG ATCAGAAGCC ATCGGTTATT TCCCTTCAGA AGCTCATTGC TAGAGAAGTC
    GCCAACGAGG AAAGAGGAAT GTTCCTGATC AGTGCATCCT CTGCTGGTCC TGAGATGTAC
    GAAATTCACA CCAACTCCAA GGAGGAACGA AATAACTGGA TGAGACGAAT CCAACAGGCC
    GTGGAAAGTT GCCCAGAAGA AGAAGGGGGA AGGACAAGTG AATCTGATGA AGAGAGGCGG
    AAAGCTGAAG CAAGAGTGGC CAAAATCCAG CAATGTCAAG AAATACTCAG TAACCAAGAC
    CAACAGATTT GCGCATATTT GGAGGAGAAG CTGCATATCT ACGCTGAACT TGGAGAACTG
    AGTGGATTCA AGGATGTCCA TCTAGAGCCC CACCTCCTCA TCAAACCTGA CCCAGGGGAG
    CCTCCCCAGG CAGCCTCATT ACTAGCAGCA GCCCTGAGAG AAGCGGAGAG CCTGCAGGTT
    GCCGTGAAGG CCTCACAAGC GGGTGATGCT CGCCCATCAC CTGAGGAGGG TTCTGCAGAA
    CCTGCGGTTG CAGATGCACA GGGTCCTCCC GAGGAGCCAG GATCATCTCC AGCCTCACTA
    GTCACAGAAG GGACAGGAGG AAGAGGCTGT TGGGATGTGG ATCCTGGCCC CCAGGGTGTG
    GGAACCGACT TGGCGGTCTC TGATGCAGGG GAGAAGGTGG AATATAGAAG TTTCACGGTT
    TCTTCACAGT CAGAGATTAT ACAAGCGATC CAGAATTTAA CCCGACTCCT CTATAGCCTT
    CAGGCCGCGC TGACCATCCA GGACAGCCAC ATCCAGGTGC ACAGGCTGCT GCTGCAGCAG
    GCTGGCCTGC CGGCGTCCCA AGAGGAGCAC GGCGAGCTGG CGAGGCAGCG GGAGGAGCTG
    GCCGCCCTGC CCGGGCTCCA GTGGCAGCTG CAGCAGGAGC GGCGGCGCGG CCAGCGCACC
    TGGGAGCGGC AGCAGCGCGA GCAGGAGGCC AGGGCCTGCC GGCTGCGGGA GCGCGAGCAG
    GGCTGCCGCG GCCGCGAGGA GCTGCTGCTC AGGGCCCGCG GGGAGCTGGA CCGGCAGCTG
    CGGGAGCACC GGCAGGACCT GGAGCGGCTG CGGGAGGGCC AGCGCCTGGT GGAGAGTGCG
    AGGGCCCGCA CGCGCGCCCA GCACAGCCTG CTGCGTGGCC CGAGGCCCGG GCGCCAGAGC
    AGCCTGCCCG CGGCCTTGCC TCCGGGCAGC GGCGAGGTGA TGGAACTTAA TCGATCCGAG
    AGCTTGTGTC ATGAAAATTC ATTCTTCATT AATGAAGCTT TAGTACAAAT GTCACTTAAC
    ACTTACAACA AATCAACTCC ATCAGGTGTC CACCAGGAAG CCGCTTATCC CCAGAATATA
    GCTAATTCGG ACTTGGTAAG GACTAGTGAA AGTCCCGTAG ACCTCCAGTT GGACATTTCT
    CAGCCCTTGG ATGTCAACCA CGAACTGTGG ACAGCCGCTG GGTCCTGTCA TCAGATACCC
    CCTCTCCACG AAAGCAGCAA GGATTCTTGT AAAAATGGAA GAATTTACAT CAGTCAGCTA
    TGTTTCCAGA GCCATTCGTC AGAAATGTTT TGGCGACCAG ACTTGACCAT CTCAGCTTGG
    GATAGAGAGC TTCCGAATCT GCAGAACTTC CAACCGCTGT TGGTTGCATT ATGTAGATGG
    TTGCGTTACG TCCGTACATC CCCGGGAGAT CTTGCCGTCA TCCTCGAGTC CCCTGAGCAG
    GTTTTTCAGC TAGAAAGAAA ATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013964540.1
    CDS92..5278
    Translation

    Target ORF information:

    RefSeq Version XM_014109065.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109065.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGAGTTGA GCTGCAGCGA AGTACCTCTT TATGGGCAGA TGACCGTCTA TGCAAAGTTT 
    GGGAAAAATG TTTATCTTCC TAAAGATGCC GAGTTTTACT TTATTTATAA TGGATCTCAT
    CAGAGACACG TCGTGATTGC AAAACGCACT GAGGATAATG TTCTCCAGTC CAGCATTCCA
    GGGCATGGGC TTCAGGAGAC GGTGACGGTG TCTGTATGCC TCTGCTCAGA AGGTTACTCC
    CCAGTGACCA TGGGCTCCGG CTCAGTGACC TACGTGGACA ACATGGCTTG CAGGCTGGCT
    CGTTTGCTGG TCACCCAGGC TGATCGCCTC ACAGCCAGTA GTCATCAGAC CTTTCTGACT
    CCATTTGCCC TGACGGTGGG AGCATTGCCT GCTTTGGACG AGGAGCTGGT GCTGGCTCTG
    ACCCATCTGG AGTTGCCTCT TGAGTGGAGT GTCTTGGGAA ATTCTTCACT TGAAGTTTCT
    TTATGCAGAG AGTCTCTTCT ACACCTGGCT ATGAGATGGG GCCTGCCTAA GCTCTCTCAT
    TTCCTCTTGT GCCTCCCTGG GGGAGTTCAG GCTTTGTCTT TACCCAATGA AGAAGGTGCC
    ACACCATTAG ACTTGGCTTT ACGTGCCGGA CACTCCAAGC TGGTTGAAGA CATCACAAAT
    TTCCAGGGCA GACGTTCCCC CGACCTCTCC CGAGTGCACC TCGGCGGACG TGCCTTCCTG
    CAGTATGTTC ACCCGTCGGG GACACTGACG CTGACGCTGA ACCACACAGC TGAGCATTTG
    TTGGAGGCAG ATATTAAACT CTTCAGGAAA TACTTTTTGG ACAGAGCCTT TCTCCTCAAG
    GCTCTTGACG ACCGAGAAGC TGGGCCAGAG GAGAGCCGGG GCATGCCCTC CCGGGCTGCC
    GCCACTGAGG AGGGTACGCG AGCTGCCTCC CCCGCGCCCA GGAACCCAGA AGAGATTAGG
    AATTTGGTGA CCGGCAGGCC ATCACCTGAA AAGGAGGATG TAAAGCGTGT GGACAGCCCG
    GACGTTGACC CCCATGAACA CGACGGCCGG GACAGTCTTG ATTTGGATCA CTCTTTCGAT
    GTCTTCAAAA AACCTAAGCA TCCCCCAGCG TTCTTTGCTG CAGGCGGGCT CTCTGATGTG
    CTGAATGGCG GCGATGAAGT TTACGCCAAC TGTATGGTGA TTGACCAGGT TGGTGATTTG
    GATATTAACT ACATCAATTT AGAAGGAATC TCTACAGACG CCTGTGCTGA ATCCATGGGT
    TCTACTCTGG GGCCTCAGAA CTGCGAGCAT ATCCTTGCTG CTGAAACCAG CCATGGGAAA
    TACCCACTAA GTGAGAACAA GGAAGTGGTC TCAGATACCG AAGCTCAGCA GTCCTTCCCA
    TCACCGTCTA GTTCATATGC TTCCAATTTT GATCTTTCCT TTGGTCTTCA TGGATTTGAG
    AAGGAACAAA GTCTCCTGAA GAAAAGAAGC TCCAGCCTGG ATGCCTTGGA TGCCGACAGC
    GAAGGGGAAG GGCATTCTGA GCGGTCACAC ATCTGCTACA CGCCAGCATC TCAGAGTTCC
    TCGAGGACTG GGATTCCCAG TGGGGATGAG CTGGACTCCT TCGAGACCGC CACCAAACCT
    GATTTTAGTA TCTCCAGGAC AGAGTCGCTT TCTTTATCAA GTAGCCTGCA GTTGAAGGAA
    TCATTGCTTC CTGGAGTCCG CTCACGTTCT TATTCCTGCT CATCACCCAA AATTTCTTTA
    GGAAAAACTC GATTGGTGCG TGATTTCACA GTGTGCAATT CTGGTGAAGA GCAAAGAGCC
    TTCGGCTTCC CGGAGCCCGC CAGGGAGAGG AGGATTCAAG AGGAAGAATG GGATAAATAC
    ATCATACCTG CCAAGTCAGA GTCTGAGAAA TACAAAGTGA GCCGGACCTT CAGCTTCCTC
    ATGAACAGGA TGACAAGCCC TCGGAACAAA TCTAAGACGA AAAGCAAGGA TGCCAAAGAC
    AAAGAGAAAC CGAGCCGACA TCAGTTTGTC CCCGGAACCT TCTCTGGGGT TCTCCAGTGT
    TCCGTTTGTG ATAAAACGCT CCTGGGGAAG GAGTCATCCC AGTGTTCCAA CTGTAATGCA
    AACGTCCACA AGGGTTGTAA GGACGCTGCA CCTCCGTGTA CCAAGAAATT CCAGGAGAAA
    TATAACAAGA ACAAACCGCA GACCATCCTT GGAAACTCTT CATTTCGAGA CATCCCGCAA
    CCCAGTCTCT CCTTGCACCC TTCTTCCTCC ATGCCTATCG GATTGCCCAC TGGGAGAAAA
    GAAGCTTCTG CCCAGGCCCA TCCCTTGTCC AGAAGTGTTC CAGGCACTGC CTTGGAAAGC
    TTCAGAAGAT CGGGGCTATC CCTGGAGTCT GAGAGTGACG GGAACAGCTG GAGAAGCAGG
    TCTCATTCCG ATGAGCTGTT ACAGTCCATG GGCTCTTCTC CCTCCACGGA ATCCTTCTTA
    ATGGAAGATG TCGTGGACTC TTCTCTATGG AGTGACCTCA GCAGTGACGC CCAGGAGTTG
    GAAGCAGAAT CCTGGAGTCT GGTGGTGGAC CCCTCCTTTT GCAATAAGCA GGAGAAAGAT
    GTCATCAAAA GACAGGATGT CATTTTCGAG CTTATGCAAA CAGAGATGCA TCATATCCAG
    ACCCTGCTCA TCATGTCTGA GATCTTCAGG AAAGGAATGA AGGAGGAGCT GCAGCTTGAT
    CACAGCACCG TGGATAAAAT CTTTCCCTGC TTAGATGAGT TACTTGAAAT CCACAGGCAT
    TTCTTTTATA GTATGAAGGA GCGAAGGCAG GAGTCCTGTG CTGGTGGTGA CAGGAATTTT
    GTCATCAGCC GAATTGGAGA TATTCTAGTA CAGCAGTTTT CAGAAGAAAA TGCAAATCAA
    ATGAAGAAAA TATATGGAGA ATTCTGTAGC CATCACAAAG AAGCTGTTAG CCTCTATAAA
    GAACTCCAAC AGAATAAGAA GTTTCAGAAT TTTATTAAGC TTCGAAATAG TAATCTTTTG
    GCTCGACGCC GAGGAATCCC AGAATGCATT CTGCTAGTCA CTCAGCGCAT CACAAAATAC
    CCTGTCCTGG TGGAACGGAT TTTGCAGTAC ACAAAGGAAA GAAGTGAAGA ACATCAAGAC
    TTGTGCAAAG CTCTGTGCTT AATTAAAGAC ATGATTGCAG CAGTGGATTT AAAAGTCAGC
    GAGTATGAGA AAAACCAAAA ATGGCTTGAG ATCCTAAATA AGATCGAAAA CAAAACCTAC
    ACAAAGCTCA AAAATGGCCA CGTGTTTAGG AAGCAAGCAC TGATAAGTAA AGAAAGGACT
    CTCTTATATG ATGGCCTTGT TTATTGGAAA ACTGCTACGG GTCGTTTCAA AGATATCCTG
    GCTCTACTTC TAACTGATGT GCTGCTCTTT TTACAAGAAA AAGACCAGAA ATACATCTTT
    GCAGCTGTTG ATCAGAAGCC ATCGGTTATT TCCCTTCAGA AGCTCATTGC TAGAGAAGTC
    GCCAACGAGG AAAGAGGAAT GTTCCTGATC AGTGCATCCT CTGCTGGTCC TGAGATGTAC
    GAAATTCACA CCAACTCCAA GGAGGAACGA AATAACTGGA TGAGACGAAT CCAACAGGCC
    GTGGAAAGTT GCCCAGAAGA AGAAGGGGGA AGGACAAGTG AATCTGATGA AGAGAGGCGG
    AAAGCTGAAG CAAGAGTGGC CAAAATCCAG CAATGTCAAG AAATACTCAG TAACCAAGAC
    CAACAGATTT GCGCATATTT GGAGGAGAAG CTGCATATCT ACGCTGAACT TGGAGAACTG
    AGTGGATTCA AGGATGTCCA TCTAGAGCCC CACCTCCTCA TCAAACCTGA CCCAGGGGAG
    CCTCCCCAGG CAGCCTCATT ACTAGCAGCA GCCCTGAGAG AAGCGGAGAG CCTGCAGGTT
    GCCGTGAAGG CCTCACAAGC GGGTGATGCT CGCCCATCAC CTGAGGAGGG TTCTGCAGAA
    CCTGCGGTTG CAGATGCACA GGGTCCTCCC GAGGAGCCAG GATCATCTCC AGCCTCACTA
    GTCACAGAAG GGACAGGAGG AAGAGGCTGT TGGGATGTGG ATCCTGGCCC CCAGGGTGTG
    GGAACCGACT TGGCGGTCTC TGATGCAGGG GAGAAGGTGG AATATAGAAG TTTCACGGTT
    TCTTCACAGT CAGAGATTAT ACAAGCGATC CAGAATTTAA CCCGACTCCT CTATAGCCTT
    CAGGCCGCGC TGACCATCCA GGACAGCCAC ATCCAGGTGC ACAGGCTGCT GCTGCAGCAG
    GCTGGCCTGC CGGCGTCCCA AGAGGAGCAC GGCGAGCTGG CGAGGCAGCG GGAGGAGCTG
    GCCGCCCTGC CCGGGCTCCA GTGGCAGCTG CAGCAGGAGC GGCGGCGCGG CCAGCGCACC
    TGGGAGCGGC AGCAGCGCGA GCAGGAGGCC AGGGCCTGCC GGCTGCGGGA GCGCGAGCAG
    GGCTGCCGCG GCCGCGAGGA GCTGCTGCTC AGGGCCCGCG GGGAGCTGGA CCGGCAGCTG
    CGGGAGCACC GGCAGGACCT GGAGCGGCTG CGGGAGGGCC AGCGCCTGGT GGAGAGTGCG
    AGGGCCCGCA CGCGCGCCCA GCACAGCCTG CTGCGTGGCC CGAGGCCCGG GCGCCAGAGC
    AGCCTGCCCG CGGCCTTGCC TCCGGGCAGC GGCGAGGTGA TGGAACTTAA TCGATCCGAG
    AGCTTGTGTC ATGAAAATTC ATTCTTCATT AATGAAGCTT TAGTACAAAT GTCACTTAAC
    ACTTACAACA AATCAACTCC ATCAGGTGTC CACCAGGAAG CCGCTTATCC CCAGAATATA
    GCTAATTCGG ACTTGGTAAG GACTAGTGAA AGTCCCGTAG ACCTCCAGTT GGACATTTCT
    CAGCCCTTGG ATGTCAACCA CGAACTGTGG ACAGCCGCTG GGTCCTGTCA TCAGATACCC
    CCTCTCCACG AAAGCAGCAA GGATTCTTGT AAAAATGGAA GAATTTACAT CAGTCAGCTA
    TGTTTCCAGA GCCATTCGTC AGAAATGTTT TGGCGACCAG ACTTGACCAT CTCAGCTTGG
    GATAGAGAGC TTCCGAATCT GCAGAACTTC CAACCGCTGT TGGTTGCATT ATGTAGATGG
    TTGCGTTACG TCCGTACATC CCCGGGAGAT CTTGCCGTCA TCCTCGAGTC CCCTGAGCAG
    GTTTTTCAGC TAGAAAGAAA ATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35384
    Clone ID Related Accession (Same CDS sequence) XM_014109073.1 , XM_014109073.2
    Accession Version XM_014109073.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5178bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product rho guanine nucleotide exchange factor 28 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAGCAACG TCTGGCATGG GCTTCAGGAG ACGGTGACGG TGTCTGTATG CCTCTGCTCA 
    GAAGGTTACT CCCCAGTGAC CATGGGCTCC GGCTCAGTGA CCTACGTGGA CAACATGGCT
    TGCAGGCTGG CTCGTTTGCT GGTCACCCAG GCTGATCGCC TCACAGCCAG TAGTCATCAG
    ACCTTTCTGA CTCCATTTGC CCTGACGGTG GGAGCATTGC CTGCTTTGGA CGAGGAGCTG
    GTGCTGGCTC TGACCCATCT GGAGTTGCCT CTTGAGTGGA GTGTCTTGGG AAATTCTTCA
    CTTGAAGTTT CTTTATGCAG AGAGTCTCTT CTACACCTGG CTATGAGATG GGGCCTGCCT
    AAGCTCTCTC ATTTCCTCTT GTGCCTCCCT GGGGGAGTTC AGGCTTTGTC TTTACCCAAT
    GAAGAAGGTG CCACACCATT AGACTTGGCT TTACGTGCCG GACACTCCAA GCTGGTTGAA
    GACATCACAA ATTTCCAGGG CAGACGTTCC CCCGACCTCT CCCGAGTGCA CCTCGGCGGA
    CGTGCCTTCC TGCAGTATGT TCACCCGTCG GGGACACTGA CGCTGACGCT GAACCACACA
    GCTGAGCATT TGTTGGAGGC AGATATTAAA CTCTTCAGGA AATACTTTTT GGACAGAGCC
    TTTCTCCTCA AGGCTCTTGA CGACCGAGAA GCTGGGCCAG AGGAGAGCCG GGGCATGCCC
    TCCCGGGCTG CCGCCACTGA GGAGGGTACG CGAGCTGCCT CCCCCGCGCC CAGGAACCCA
    GAAGAGATTA GGAATTTGGT GACCGGCAGG CCATCACCTG AAAAGGAGGA TGTAAAGCGT
    GTGGACAGCC CGGACGTTGA CCCCCATGAA CACGACGGCC GGGACAGTCT TGATTTGGAT
    CACTCTTTCG ATGTCTTCAA AAAACCTAAG CATCCCCCAG CGTTCTTTGC TGCAGGCGGG
    CTCTCTGATG TGCTGAATGG CGGCGATGAA GTTTACGCCA ACTGTATGGT GATTGACCAG
    GTTGGTGATT TGGATATTAA CTACATCAAT TTAGAAGGAA TCTCTACAGA CGCCTGTGCT
    GAATCCATGG GTTCTACTCT GGGGCCTCAG AACTGCGAGC ATATCCTTGC TGCTGAAACC
    AGCCATGGGA AATACCCACT AAGTGAGAAC AAGGAAGTGG TCTCAGATAC CGAAGCTCAG
    CAGTCCTTCC CATCACCGTC TAGTTCATAT GCTTCCAATT TTGATCTTTC CTTTGGTCTT
    CATGGATTTG AGAAGGAACA AAGTCTCCTG AAGAAAAGAA GCTCCAGCCT GGATGCCTTG
    GATGCCGACA GCGAAGGGGA AGGGCATTCT GAGCGGTCAC ACATCTGCTA CACGCCAGCA
    TCTCAGAGTT CCTCGAGGAC TGGGATTCCC AGTGGGGATG AGCTGGACTC CTTCGAGACC
    GCCACCAAAC CTGATTTTAG TATCTCCAGG ACAGAGTCGC TTTCTTTATC AAGTAGCCTG
    CAGTTGAAGG AATCATTGCT TCCTGGAGTC CGCTCACGTT CTTATTCCTG CTCATCACCC
    AAAATTTCTT TAGGAAAAAC TCGATTGGTG CGTGATTTCA CAGTGTGCAA TTCTGGTGAA
    GAGCAAAGAG CCTTCGGCTT CCCGGAGCCC GCCAGGGAGA GGAGGATTCA AGAGGAAGAA
    TGGGATAAAT ACATCATACC TGCCAAGTCA GAGTCTGAGA AATACAAAGT GAGCCGGACC
    TTCAGCTTCC TCATGAACAG GATGACAAGC CCTCGGAACA AATCTAAGAC GAAAAGCAAG
    GATGCCAAAG ACAAAGAGAA ACCGAGCCGA CATCAGTTTG TCCCCGGAAC CTTCTCTGGG
    GTTCTCCAGT GTTCCGTTTG TGATAAAACG CTCCTGGGGA AGGAGTCATC CCAGTGTTCC
    AACTGTAATG CAAACGTCCA CAAGGGTTGT AAGGACGCTG CACCTCCGTG TACCAAGAAA
    TTCCAGGAGA AATATAACAA GAACAAACCG CAGACCATCC TTGGAAACTC TTCATTTCGA
    GACATCCCGC AACCCAGTCT CTCCTTGCAC CCTTCTTCCT CCATGCCTAT CGGATTGCCC
    ACTGGGAGAA AAGAAGCTTC TGCCCAGGCC CATCCCTTGT CCAGAAGTGT TCCAGGCACT
    GCCTTGGAAA GCTTCAGAAG ATCGGGGCTA TCCCTGGAGT CTGAGAGTGA CGGGAACAGC
    TGGAGAAGCA GGTCTCATTC CGATGAGCTG TTACAGTCCA TGGGCTCTTC TCCCTCCACG
    GAATCCTTCT TAATGGAAGA TGTCGTGGAC TCTTCTCTAT GGAGTGACCT CAGCAGTGAC
    GCCCAGGAGT TGGAAGCAGA ATCCTGGAGT CTGGTGGTGG ACCCCTCCTT TTGCAATAAG
    CAGGAGAAAG ATGTCATCAA AAGACAGGAT GTCATTTTCG AGCTTATGCA AACAGAGATG
    CATCATATCC AGACCCTGCT CATCATGTCT GAGATCTTCA GGAAAGGAAT GAAGGAGGAG
    CTGCAGCTTG ATCACAGCAC CGTGGATAAA ATCTTTCCCT GCTTAGATGA GTTACTTGAA
    ATCCACAGGC ATTTCTTTTA TAGTATGAAG GAGCGAAGGC AGGAGTCCTG TGCTGGTGGT
    GACAGGAATT TTGTCATCAG CCGAATTGGA GATATTCTAG TACAGCAGTT TTCAGAAGAA
    AATGCAAATC AAATGAAGAA AATATATGGA GAATTCTGTA GCCATCACAA AGAAGCTGTT
    AGCCTCTATA AAGAACTCCA ACAGAATAAG AAGTTTCAGA ATTTTATTAA GCTTCGAAAT
    AGTAATCTTT TGGCTCGACG CCGAGGAATC CCAGAATGCA TTCTGCTAGT CACTCAGCGC
    ATCACAAAAT ACCCTGTCCT GGTGGAACGG ATTTTGCAGT ACACAAAGGA AAGAAGTGAA
    GAACATCAAG ACTTGTGCAA AGCTCTGTGC TTAATTAAAG ACATGATTGC AGCAGTGGAT
    TTAAAAGTCA GCGAGTATGA GAAAAACCAA AAATGGCTTG AGATCCTAAA TAAGATCGAA
    AACAAAACCT ACACAAAGCT CAAAAATGGC CACGTGTTTA GGAAGCAAGC ACTGATAAGT
    AAAGAAAGGA CTCTCTTATA TGATGGCCTT GTTTATTGGA AAACTGCTAC GGGTCGTTTC
    AAAGATATCC TGGCTCTACT TCTAACTGAT GTGCTGCTCT TTTTACAAGA AAAAGACCAG
    AAATACATCT TTGCAGCTGT TGATCAGAAG CCATCGGTTA TTTCCCTTCA GAAGCTCATT
    GCTAGAGAAG TCGCCAACGA GGAAAGAGGA ATGTTCCTGA TCAGTGCATC CTCTGCTGGT
    CCTGAGATGT ACGAAATTCA CACCAACTCC AAGGAGGAAC GAAATAACTG GATGAGACGA
    ATCCAACAGG CCGTGGAAAG TTGCCCAGAA GAAGAAGGGG GAAGGACAAG TGAATCTGAT
    GAAGAGAGGC GGAAAGCTGA AGCAAGAGTG GCCAAAATCC AGCAATGTCA AGAAATACTC
    AGTAACCAAG ACCAACAGAT TTGCGCATAT TTGGAGGAGA AGCTGCATAT CTACGCTGAA
    CTTGGAGAAC TGAGTGGATT CAAGGATGTC CATCTAGAGC CCCACCTCCT CATCAAACCT
    GACCCAGGGG AGCCTCCCCA GGCAGCCTCA TTACTAGCAG CAGCCCTGAG AGAAGCGGAG
    AGCCTGCAGG TTGCCGTGAA GGCCTCACAA GCGGGTGATG CTCGCCCATC ACCTGAGGAG
    GGTTCTGCAG AACCTGCGGT TGCAGATGCA CAGGGTCCTC CCGAGGAGCC AGGATCATCT
    CCAGCCTCAC TAGTCACAGA AGGGACAGGA GGAAGAGGCT GTTGGGATGT GGATCCTGGC
    CCCCAGGGTG TGGGAACCGA CTTGGCGGTC TCTGATGCAG GGGAGAAGAG TCTTAAAAAT
    CTGTGCTGCA GCCGGCAAAG TCCCTGGGGT GTCAGTCACC ATCACCAGCT GCAGCCCTGC
    ACAGGGACCA CAAGGGGGAG GGCCAGCTCC ACGAAAAGTA GGACACTACA CAGCTCAGGC
    TTGTATCAGG CTTCGTGGTT CTTGGGAGTG GAATATAGAA GTTTCACGGT TTCTTCACAG
    TCAGAGATTA TACAAGCGAT CCAGAATTTA ACCCGACTCC TCTATAGCCT TCAGGCCGCG
    CTGACCATCC AGGACAGCCA CATCCAGGTG CACAGGCTGC TGCTGCAGCA GGCTGGCCTG
    CCGGCGTCCC AAGAGGAGCA CGGCGAGCTG GCGAGGCAGC GGGAGGAGCT GGCCGCCCTG
    CCCGGGCTCC AGTGGCAGCT GCAGCAGGAG CGGCGGCGCG GCCAGCGCAC CTGGGAGCGG
    CAGCAGCGCG AGCAGGAGGC CAGGGCCTGC CGGCTGCGGG AGCGCGAGCA GGGCTGCCGC
    GGCCGCGAGG AGCTGCTGCT CAGGGCCCGC GGGGAGCTGG ACCGGCAGCT GCGGGAGCAC
    CGGCAGGACC TGGAGCGGCT GCGGGAGGGC CAGCGCCTGG TGGAGAGTGC GAGGGCCCGC
    ACGCGCGCCC AGCACAGCCT GCTGCGTGGC CCGAGGCCCG GGCGCCAGAG CAGCCTGCCC
    GCGGCCTTGC CTCCGGGCAG CGGCGAGGTG ATGGAACTTA ATCGATCCGA GAGCTTGTGT
    CATGAAAATT CATTCTTCAT TAATGAAGCT TTAGTACAAA TGTCACTTAA CACTTACAAC
    AAATCAACTC CATCAGGTGT CCACCAGGAA GCCGCTTATC CCCAGAATAT AGCTAATTCG
    GACTTGGTAA GGACTAGTGA AAGTCCCGTA GACCTCCAGT TGGACATTTC TCAGCCCTTG
    GATGTCAACC ACGAACTGTG GACAGCCGCT GGGTCCTGTC ATCAGATACC CCCTCTCCAC
    GAAAGCAGCA AGGATTCTTG TAAAAATGGA AGAATTTACA TCAGTCAGCT ATGTTTCCAG
    AGCCATTCGT CAGAAATGTT TTGGCGACCA GACTTGACCA TCTCAGCTTG GGATAGAGAG
    CTTCCGAATC TGCAGAACTT CCAACCGCTG TTGGTTGCAT TATGTAGATG GTTGCGTTAC
    GTCCGTACAT CCCCGGGAGA TCTTGCCGTC ATCCTCGAGT CCCCTGAGCA GGTTTTTCAG
    CTAGAAAGAA AATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013964548.1
    CDS31..5208
    Translation

    Target ORF information:

    RefSeq Version XM_014109073.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 28 (ARHGEF28), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109073.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAGCAACG TCTGGCATGG GCTTCAGGAG ACGGTGACGG TGTCTGTATG CCTCTGCTCA 
    GAAGGTTACT CCCCAGTGAC CATGGGCTCC GGCTCAGTGA CCTACGTGGA CAACATGGCT
    TGCAGGCTGG CTCGTTTGCT GGTCACCCAG GCTGATCGCC TCACAGCCAG TAGTCATCAG
    ACCTTTCTGA CTCCATTTGC CCTGACGGTG GGAGCATTGC CTGCTTTGGA CGAGGAGCTG
    GTGCTGGCTC TGACCCATCT GGAGTTGCCT CTTGAGTGGA GTGTCTTGGG AAATTCTTCA
    CTTGAAGTTT CTTTATGCAG AGAGTCTCTT CTACACCTGG CTATGAGATG GGGCCTGCCT
    AAGCTCTCTC ATTTCCTCTT GTGCCTCCCT GGGGGAGTTC AGGCTTTGTC TTTACCCAAT
    GAAGAAGGTG CCACACCATT AGACTTGGCT TTACGTGCCG GACACTCCAA GCTGGTTGAA
    GACATCACAA ATTTCCAGGG CAGACGTTCC CCCGACCTCT CCCGAGTGCA CCTCGGCGGA
    CGTGCCTTCC TGCAGTATGT TCACCCGTCG GGGACACTGA CGCTGACGCT GAACCACACA
    GCTGAGCATT TGTTGGAGGC AGATATTAAA CTCTTCAGGA AATACTTTTT GGACAGAGCC
    TTTCTCCTCA AGGCTCTTGA CGACCGAGAA GCTGGGCCAG AGGAGAGCCG GGGCATGCCC
    TCCCGGGCTG CCGCCACTGA GGAGGGTACG CGAGCTGCCT CCCCCGCGCC CAGGAACCCA
    GAAGAGATTA GGAATTTGGT GACCGGCAGG CCATCACCTG AAAAGGAGGA TGTAAAGCGT
    GTGGACAGCC CGGACGTTGA CCCCCATGAA CACGACGGCC GGGACAGTCT TGATTTGGAT
    CACTCTTTCG ATGTCTTCAA AAAACCTAAG CATCCCCCAG CGTTCTTTGC TGCAGGCGGG
    CTCTCTGATG TGCTGAATGG CGGCGATGAA GTTTACGCCA ACTGTATGGT GATTGACCAG
    GTTGGTGATT TGGATATTAA CTACATCAAT TTAGAAGGAA TCTCTACAGA CGCCTGTGCT
    GAATCCATGG GTTCTACTCT GGGGCCTCAG AACTGCGAGC ATATCCTTGC TGCTGAAACC
    AGCCATGGGA AATACCCACT AAGTGAGAAC AAGGAAGTGG TCTCAGATAC CGAAGCTCAG
    CAGTCCTTCC CATCACCGTC TAGTTCATAT GCTTCCAATT TTGATCTTTC CTTTGGTCTT
    CATGGATTTG AGAAGGAACA AAGTCTCCTG AAGAAAAGAA GCTCCAGCCT GGATGCCTTG
    GATGCCGACA GCGAAGGGGA AGGGCATTCT GAGCGGTCAC ACATCTGCTA CACGCCAGCA
    TCTCAGAGTT CCTCGAGGAC TGGGATTCCC AGTGGGGATG AGCTGGACTC CTTCGAGACC
    GCCACCAAAC CTGATTTTAG TATCTCCAGG ACAGAGTCGC TTTCTTTATC AAGTAGCCTG
    CAGTTGAAGG AATCATTGCT TCCTGGAGTC CGCTCACGTT CTTATTCCTG CTCATCACCC
    AAAATTTCTT TAGGAAAAAC TCGATTGGTG CGTGATTTCA CAGTGTGCAA TTCTGGTGAA
    GAGCAAAGAG CCTTCGGCTT CCCGGAGCCC GCCAGGGAGA GGAGGATTCA AGAGGAAGAA
    TGGGATAAAT ACATCATACC TGCCAAGTCA GAGTCTGAGA AATACAAAGT GAGCCGGACC
    TTCAGCTTCC TCATGAACAG GATGACAAGC CCTCGGAACA AATCTAAGAC GAAAAGCAAG
    GATGCCAAAG ACAAAGAGAA ACCGAGCCGA CATCAGTTTG TCCCCGGAAC CTTCTCTGGG
    GTTCTCCAGT GTTCCGTTTG TGATAAAACG CTCCTGGGGA AGGAGTCATC CCAGTGTTCC
    AACTGTAATG CAAACGTCCA CAAGGGTTGT AAGGACGCTG CACCTCCGTG TACCAAGAAA
    TTCCAGGAGA AATATAACAA GAACAAACCG CAGACCATCC TTGGAAACTC TTCATTTCGA
    GACATCCCGC AACCCAGTCT CTCCTTGCAC CCTTCTTCCT CCATGCCTAT CGGATTGCCC
    ACTGGGAGAA AAGAAGCTTC TGCCCAGGCC CATCCCTTGT CCAGAAGTGT TCCAGGCACT
    GCCTTGGAAA GCTTCAGAAG ATCGGGGCTA TCCCTGGAGT CTGAGAGTGA CGGGAACAGC
    TGGAGAAGCA GGTCTCATTC CGATGAGCTG TTACAGTCCA TGGGCTCTTC TCCCTCCACG
    GAATCCTTCT TAATGGAAGA TGTCGTGGAC TCTTCTCTAT GGAGTGACCT CAGCAGTGAC
    GCCCAGGAGT TGGAAGCAGA ATCCTGGAGT CTGGTGGTGG ACCCCTCCTT TTGCAATAAG
    CAGGAGAAAG ATGTCATCAA AAGACAGGAT GTCATTTTCG AGCTTATGCA AACAGAGATG
    CATCATATCC AGACCCTGCT CATCATGTCT GAGATCTTCA GGAAAGGAAT GAAGGAGGAG
    CTGCAGCTTG ATCACAGCAC CGTGGATAAA ATCTTTCCCT GCTTAGATGA GTTACTTGAA
    ATCCACAGGC ATTTCTTTTA TAGTATGAAG GAGCGAAGGC AGGAGTCCTG TGCTGGTGGT
    GACAGGAATT TTGTCATCAG CCGAATTGGA GATATTCTAG TACAGCAGTT TTCAGAAGAA
    AATGCAAATC AAATGAAGAA AATATATGGA GAATTCTGTA GCCATCACAA AGAAGCTGTT
    AGCCTCTATA AAGAACTCCA ACAGAATAAG AAGTTTCAGA ATTTTATTAA GCTTCGAAAT
    AGTAATCTTT TGGCTCGACG CCGAGGAATC CCAGAATGCA TTCTGCTAGT CACTCAGCGC
    ATCACAAAAT ACCCTGTCCT GGTGGAACGG ATTTTGCAGT ACACAAAGGA AAGAAGTGAA
    GAACATCAAG ACTTGTGCAA AGCTCTGTGC TTAATTAAAG ACATGATTGC AGCAGTGGAT
    TTAAAAGTCA GCGAGTATGA GAAAAACCAA AAATGGCTTG AGATCCTAAA TAAGATCGAA
    AACAAAACCT ACACAAAGCT CAAAAATGGC CACGTGTTTA GGAAGCAAGC ACTGATAAGT
    AAAGAAAGGA CTCTCTTATA TGATGGCCTT GTTTATTGGA AAACTGCTAC GGGTCGTTTC
    AAAGATATCC TGGCTCTACT TCTAACTGAT GTGCTGCTCT TTTTACAAGA AAAAGACCAG
    AAATACATCT TTGCAGCTGT TGATCAGAAG CCATCGGTTA TTTCCCTTCA GAAGCTCATT
    GCTAGAGAAG TCGCCAACGA GGAAAGAGGA ATGTTCCTGA TCAGTGCATC CTCTGCTGGT
    CCTGAGATGT ACGAAATTCA CACCAACTCC AAGGAGGAAC GAAATAACTG GATGAGACGA
    ATCCAACAGG CCGTGGAAAG TTGCCCAGAA GAAGAAGGGG GAAGGACAAG TGAATCTGAT
    GAAGAGAGGC GGAAAGCTGA AGCAAGAGTG GCCAAAATCC AGCAATGTCA AGAAATACTC
    AGTAACCAAG ACCAACAGAT TTGCGCATAT TTGGAGGAGA AGCTGCATAT CTACGCTGAA
    CTTGGAGAAC TGAGTGGATT CAAGGATGTC CATCTAGAGC CCCACCTCCT CATCAAACCT
    GACCCAGGGG AGCCTCCCCA GGCAGCCTCA TTACTAGCAG CAGCCCTGAG AGAAGCGGAG
    AGCCTGCAGG TTGCCGTGAA GGCCTCACAA GCGGGTGATG CTCGCCCATC ACCTGAGGAG
    GGTTCTGCAG AACCTGCGGT TGCAGATGCA CAGGGTCCTC CCGAGGAGCC AGGATCATCT
    CCAGCCTCAC TAGTCACAGA AGGGACAGGA GGAAGAGGCT GTTGGGATGT GGATCCTGGC
    CCCCAGGGTG TGGGAACCGA CTTGGCGGTC TCTGATGCAG GGGAGAAGAG TCTTAAAAAT
    CTGTGCTGCA GCCGGCAAAG TCCCTGGGGT GTCAGTCACC ATCACCAGCT GCAGCCCTGC
    ACAGGGACCA CAAGGGGGAG GGCCAGCTCC ACGAAAAGTA GGACACTACA CAGCTCAGGC
    TTGTATCAGG CTTCGTGGTT CTTGGGAGTG GAATATAGAA GTTTCACGGT TTCTTCACAG
    TCAGAGATTA TACAAGCGAT CCAGAATTTA ACCCGACTCC TCTATAGCCT TCAGGCCGCG
    CTGACCATCC AGGACAGCCA CATCCAGGTG CACAGGCTGC TGCTGCAGCA GGCTGGCCTG
    CCGGCGTCCC AAGAGGAGCA CGGCGAGCTG GCGAGGCAGC GGGAGGAGCT GGCCGCCCTG
    CCCGGGCTCC AGTGGCAGCT GCAGCAGGAG CGGCGGCGCG GCCAGCGCAC CTGGGAGCGG
    CAGCAGCGCG AGCAGGAGGC CAGGGCCTGC CGGCTGCGGG AGCGCGAGCA GGGCTGCCGC
    GGCCGCGAGG AGCTGCTGCT CAGGGCCCGC GGGGAGCTGG ACCGGCAGCT GCGGGAGCAC
    CGGCAGGACC TGGAGCGGCT GCGGGAGGGC CAGCGCCTGG TGGAGAGTGC GAGGGCCCGC
    ACGCGCGCCC AGCACAGCCT GCTGCGTGGC CCGAGGCCCG GGCGCCAGAG CAGCCTGCCC
    GCGGCCTTGC CTCCGGGCAG CGGCGAGGTG ATGGAACTTA ATCGATCCGA GAGCTTGTGT
    CATGAAAATT CATTCTTCAT TAATGAAGCT TTAGTACAAA TGTCACTTAA CACTTACAAC
    AAATCAACTC CATCAGGTGT CCACCAGGAA GCCGCTTATC CCCAGAATAT AGCTAATTCG
    GACTTGGTAA GGACTAGTGA AAGTCCCGTA GACCTCCAGT TGGACATTTC TCAGCCCTTG
    GATGTCAACC ACGAACTGTG GACAGCCGCT GGGTCCTGTC ATCAGATACC CCCTCTCCAC
    GAAAGCAGCA AGGATTCTTG TAAAAATGGA AGAATTTACA TCAGTCAGCT ATGTTTCCAG
    AGCCATTCGT CAGAAATGTT TTGGCGACCA GACTTGACCA TCTCAGCTTG GGATAGAGAG
    CTTCCGAATC TGCAGAACTT CCAACCGCTG TTGGTTGCAT TATGTAGATG GTTGCGTTAC
    GTCCGTACAT CCCCGGGAGA TCTTGCCGTC ATCCTCGAGT CCCCTGAGCA GGTTTTTCAG
    CTAGAAAGAA AATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35384
    Clone ID Related Accession (Same CDS sequence) XM_014109073.1 , XM_014109073.2
    Accession Version XM_014109073.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5178bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rho guanine nucleotide exchange factor 28 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109073.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAGCAACG TCTGGCATGG GCTTCAGGAG ACGGTGACGG TGTCTGTATG CCTCTGCTCA 
    GAAGGTTACT CCCCAGTGAC CATGGGCTCC GGCTCAGTGA CCTACGTGGA CAACATGGCT
    TGCAGGCTGG CTCGTTTGCT GGTCACCCAG GCTGATCGCC TCACAGCCAG TAGTCATCAG
    ACCTTTCTGA CTCCATTTGC CCTGACGGTG GGAGCATTGC CTGCTTTGGA CGAGGAGCTG
    GTGCTGGCTC TGACCCATCT GGAGTTGCCT CTTGAGTGGA GTGTCTTGGG AAATTCTTCA
    CTTGAAGTTT CTTTATGCAG AGAGTCTCTT CTACACCTGG CTATGAGATG GGGCCTGCCT
    AAGCTCTCTC ATTTCCTCTT GTGCCTCCCT GGGGGAGTTC AGGCTTTGTC TTTACCCAAT
    GAAGAAGGTG CCACACCATT AGACTTGGCT TTACGTGCCG GACACTCCAA GCTGGTTGAA
    GACATCACAA ATTTCCAGGG CAGACGTTCC CCCGACCTCT CCCGAGTGCA CCTCGGCGGA
    CGTGCCTTCC TGCAGTATGT TCACCCGTCG GGGACACTGA CGCTGACGCT GAACCACACA
    GCTGAGCATT TGTTGGAGGC AGATATTAAA CTCTTCAGGA AATACTTTTT GGACAGAGCC
    TTTCTCCTCA AGGCTCTTGA CGACCGAGAA GCTGGGCCAG AGGAGAGCCG GGGCATGCCC
    TCCCGGGCTG CCGCCACTGA GGAGGGTACG CGAGCTGCCT CCCCCGCGCC CAGGAACCCA
    GAAGAGATTA GGAATTTGGT GACCGGCAGG CCATCACCTG AAAAGGAGGA TGTAAAGCGT
    GTGGACAGCC CGGACGTTGA CCCCCATGAA CACGACGGCC GGGACAGTCT TGATTTGGAT
    CACTCTTTCG ATGTCTTCAA AAAACCTAAG CATCCCCCAG CGTTCTTTGC TGCAGGCGGG
    CTCTCTGATG TGCTGAATGG CGGCGATGAA GTTTACGCCA ACTGTATGGT GATTGACCAG
    GTTGGTGATT TGGATATTAA CTACATCAAT TTAGAAGGAA TCTCTACAGA CGCCTGTGCT
    GAATCCATGG GTTCTACTCT GGGGCCTCAG AACTGCGAGC ATATCCTTGC TGCTGAAACC
    AGCCATGGGA AATACCCACT AAGTGAGAAC AAGGAAGTGG TCTCAGATAC CGAAGCTCAG
    CAGTCCTTCC CATCACCGTC TAGTTCATAT GCTTCCAATT TTGATCTTTC CTTTGGTCTT
    CATGGATTTG AGAAGGAACA AAGTCTCCTG AAGAAAAGAA GCTCCAGCCT GGATGCCTTG
    GATGCCGACA GCGAAGGGGA AGGGCATTCT GAGCGGTCAC ACATCTGCTA CACGCCAGCA
    TCTCAGAGTT CCTCGAGGAC TGGGATTCCC AGTGGGGATG AGCTGGACTC CTTCGAGACC
    GCCACCAAAC CTGATTTTAG TATCTCCAGG ACAGAGTCGC TTTCTTTATC AAGTAGCCTG
    CAGTTGAAGG AATCATTGCT TCCTGGAGTC CGCTCACGTT CTTATTCCTG CTCATCACCC
    AAAATTTCTT TAGGAAAAAC TCGATTGGTG CGTGATTTCA CAGTGTGCAA TTCTGGTGAA
    GAGCAAAGAG CCTTCGGCTT CCCGGAGCCC GCCAGGGAGA GGAGGATTCA AGAGGAAGAA
    TGGGATAAAT ACATCATACC TGCCAAGTCA GAGTCTGAGA AATACAAAGT GAGCCGGACC
    TTCAGCTTCC TCATGAACAG GATGACAAGC CCTCGGAACA AATCTAAGAC GAAAAGCAAG
    GATGCCAAAG ACAAAGAGAA ACCGAGCCGA CATCAGTTTG TCCCCGGAAC CTTCTCTGGG
    GTTCTCCAGT GTTCCGTTTG TGATAAAACG CTCCTGGGGA AGGAGTCATC CCAGTGTTCC
    AACTGTAATG CAAACGTCCA CAAGGGTTGT AAGGACGCTG CACCTCCGTG TACCAAGAAA
    TTCCAGGAGA AATATAACAA GAACAAACCG CAGACCATCC TTGGAAACTC TTCATTTCGA
    GACATCCCGC AACCCAGTCT CTCCTTGCAC CCTTCTTCCT CCATGCCTAT CGGATTGCCC
    ACTGGGAGAA AAGAAGCTTC TGCCCAGGCC CATCCCTTGT CCAGAAGTGT TCCAGGCACT
    GCCTTGGAAA GCTTCAGAAG ATCGGGGCTA TCCCTGGAGT CTGAGAGTGA CGGGAACAGC
    TGGAGAAGCA GGTCTCATTC CGATGAGCTG TTACAGTCCA TGGGCTCTTC TCCCTCCACG
    GAATCCTTCT TAATGGAAGA TGTCGTGGAC TCTTCTCTAT GGAGTGACCT CAGCAGTGAC
    GCCCAGGAGT TGGAAGCAGA ATCCTGGAGT CTGGTGGTGG ACCCCTCCTT TTGCAATAAG
    CAGGAGAAAG ATGTCATCAA AAGACAGGAT GTCATTTTCG AGCTTATGCA AACAGAGATG
    CATCATATCC AGACCCTGCT CATCATGTCT GAGATCTTCA GGAAAGGAAT GAAGGAGGAG
    CTGCAGCTTG ATCACAGCAC CGTGGATAAA ATCTTTCCCT GCTTAGATGA GTTACTTGAA
    ATCCACAGGC ATTTCTTTTA TAGTATGAAG GAGCGAAGGC AGGAGTCCTG TGCTGGTGGT
    GACAGGAATT TTGTCATCAG CCGAATTGGA GATATTCTAG TACAGCAGTT TTCAGAAGAA
    AATGCAAATC AAATGAAGAA AATATATGGA GAATTCTGTA GCCATCACAA AGAAGCTGTT
    AGCCTCTATA AAGAACTCCA ACAGAATAAG AAGTTTCAGA ATTTTATTAA GCTTCGAAAT
    AGTAATCTTT TGGCTCGACG CCGAGGAATC CCAGAATGCA TTCTGCTAGT CACTCAGCGC
    ATCACAAAAT ACCCTGTCCT GGTGGAACGG ATTTTGCAGT ACACAAAGGA AAGAAGTGAA
    GAACATCAAG ACTTGTGCAA AGCTCTGTGC TTAATTAAAG ACATGATTGC AGCAGTGGAT
    TTAAAAGTCA GCGAGTATGA GAAAAACCAA AAATGGCTTG AGATCCTAAA TAAGATCGAA
    AACAAAACCT ACACAAAGCT CAAAAATGGC CACGTGTTTA GGAAGCAAGC ACTGATAAGT
    AAAGAAAGGA CTCTCTTATA TGATGGCCTT GTTTATTGGA AAACTGCTAC GGGTCGTTTC
    AAAGATATCC TGGCTCTACT TCTAACTGAT GTGCTGCTCT TTTTACAAGA AAAAGACCAG
    AAATACATCT TTGCAGCTGT TGATCAGAAG CCATCGGTTA TTTCCCTTCA GAAGCTCATT
    GCTAGAGAAG TCGCCAACGA GGAAAGAGGA ATGTTCCTGA TCAGTGCATC CTCTGCTGGT
    CCTGAGATGT ACGAAATTCA CACCAACTCC AAGGAGGAAC GAAATAACTG GATGAGACGA
    ATCCAACAGG CCGTGGAAAG TTGCCCAGAA GAAGAAGGGG GAAGGACAAG TGAATCTGAT
    GAAGAGAGGC GGAAAGCTGA AGCAAGAGTG GCCAAAATCC AGCAATGTCA AGAAATACTC
    AGTAACCAAG ACCAACAGAT TTGCGCATAT TTGGAGGAGA AGCTGCATAT CTACGCTGAA
    CTTGGAGAAC TGAGTGGATT CAAGGATGTC CATCTAGAGC CCCACCTCCT CATCAAACCT
    GACCCAGGGG AGCCTCCCCA GGCAGCCTCA TTACTAGCAG CAGCCCTGAG AGAAGCGGAG
    AGCCTGCAGG TTGCCGTGAA GGCCTCACAA GCGGGTGATG CTCGCCCATC ACCTGAGGAG
    GGTTCTGCAG AACCTGCGGT TGCAGATGCA CAGGGTCCTC CCGAGGAGCC AGGATCATCT
    CCAGCCTCAC TAGTCACAGA AGGGACAGGA GGAAGAGGCT GTTGGGATGT GGATCCTGGC
    CCCCAGGGTG TGGGAACCGA CTTGGCGGTC TCTGATGCAG GGGAGAAGAG TCTTAAAAAT
    CTGTGCTGCA GCCGGCAAAG TCCCTGGGGT GTCAGTCACC ATCACCAGCT GCAGCCCTGC
    ACAGGGACCA CAAGGGGGAG GGCCAGCTCC ACGAAAAGTA GGACACTACA CAGCTCAGGC
    TTGTATCAGG CTTCGTGGTT CTTGGGAGTG GAATATAGAA GTTTCACGGT TTCTTCACAG
    TCAGAGATTA TACAAGCGAT CCAGAATTTA ACCCGACTCC TCTATAGCCT TCAGGCCGCG
    CTGACCATCC AGGACAGCCA CATCCAGGTG CACAGGCTGC TGCTGCAGCA GGCTGGCCTG
    CCGGCGTCCC AAGAGGAGCA CGGCGAGCTG GCGAGGCAGC GGGAGGAGCT GGCCGCCCTG
    CCCGGGCTCC AGTGGCAGCT GCAGCAGGAG CGGCGGCGCG GCCAGCGCAC CTGGGAGCGG
    CAGCAGCGCG AGCAGGAGGC CAGGGCCTGC CGGCTGCGGG AGCGCGAGCA GGGCTGCCGC
    GGCCGCGAGG AGCTGCTGCT CAGGGCCCGC GGGGAGCTGG ACCGGCAGCT GCGGGAGCAC
    CGGCAGGACC TGGAGCGGCT GCGGGAGGGC CAGCGCCTGG TGGAGAGTGC GAGGGCCCGC
    ACGCGCGCCC AGCACAGCCT GCTGCGTGGC CCGAGGCCCG GGCGCCAGAG CAGCCTGCCC
    GCGGCCTTGC CTCCGGGCAG CGGCGAGGTG ATGGAACTTA ATCGATCCGA GAGCTTGTGT
    CATGAAAATT CATTCTTCAT TAATGAAGCT TTAGTACAAA TGTCACTTAA CACTTACAAC
    AAATCAACTC CATCAGGTGT CCACCAGGAA GCCGCTTATC CCCAGAATAT AGCTAATTCG
    GACTTGGTAA GGACTAGTGA AAGTCCCGTA GACCTCCAGT TGGACATTTC TCAGCCCTTG
    GATGTCAACC ACGAACTGTG GACAGCCGCT GGGTCCTGTC ATCAGATACC CCCTCTCCAC
    GAAAGCAGCA AGGATTCTTG TAAAAATGGA AGAATTTACA TCAGTCAGCT ATGTTTCCAG
    AGCCATTCGT CAGAAATGTT TTGGCGACCA GACTTGACCA TCTCAGCTTG GGATAGAGAG
    CTTCCGAATC TGCAGAACTT CCAACCGCTG TTGGTTGCAT TATGTAGATG GTTGCGTTAC
    GTCCGTACAT CCCCGGGAGA TCTTGCCGTC ATCCTCGAGT CCCCTGAGCA GGTTTTTCAG
    CTAGAAAGAA AATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013964548.1
    CDS72..5249
    Translation

    Target ORF information:

    RefSeq Version XM_014109073.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109073.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAGCAACG TCTGGCATGG GCTTCAGGAG ACGGTGACGG TGTCTGTATG CCTCTGCTCA 
    GAAGGTTACT CCCCAGTGAC CATGGGCTCC GGCTCAGTGA CCTACGTGGA CAACATGGCT
    TGCAGGCTGG CTCGTTTGCT GGTCACCCAG GCTGATCGCC TCACAGCCAG TAGTCATCAG
    ACCTTTCTGA CTCCATTTGC CCTGACGGTG GGAGCATTGC CTGCTTTGGA CGAGGAGCTG
    GTGCTGGCTC TGACCCATCT GGAGTTGCCT CTTGAGTGGA GTGTCTTGGG AAATTCTTCA
    CTTGAAGTTT CTTTATGCAG AGAGTCTCTT CTACACCTGG CTATGAGATG GGGCCTGCCT
    AAGCTCTCTC ATTTCCTCTT GTGCCTCCCT GGGGGAGTTC AGGCTTTGTC TTTACCCAAT
    GAAGAAGGTG CCACACCATT AGACTTGGCT TTACGTGCCG GACACTCCAA GCTGGTTGAA
    GACATCACAA ATTTCCAGGG CAGACGTTCC CCCGACCTCT CCCGAGTGCA CCTCGGCGGA
    CGTGCCTTCC TGCAGTATGT TCACCCGTCG GGGACACTGA CGCTGACGCT GAACCACACA
    GCTGAGCATT TGTTGGAGGC AGATATTAAA CTCTTCAGGA AATACTTTTT GGACAGAGCC
    TTTCTCCTCA AGGCTCTTGA CGACCGAGAA GCTGGGCCAG AGGAGAGCCG GGGCATGCCC
    TCCCGGGCTG CCGCCACTGA GGAGGGTACG CGAGCTGCCT CCCCCGCGCC CAGGAACCCA
    GAAGAGATTA GGAATTTGGT GACCGGCAGG CCATCACCTG AAAAGGAGGA TGTAAAGCGT
    GTGGACAGCC CGGACGTTGA CCCCCATGAA CACGACGGCC GGGACAGTCT TGATTTGGAT
    CACTCTTTCG ATGTCTTCAA AAAACCTAAG CATCCCCCAG CGTTCTTTGC TGCAGGCGGG
    CTCTCTGATG TGCTGAATGG CGGCGATGAA GTTTACGCCA ACTGTATGGT GATTGACCAG
    GTTGGTGATT TGGATATTAA CTACATCAAT TTAGAAGGAA TCTCTACAGA CGCCTGTGCT
    GAATCCATGG GTTCTACTCT GGGGCCTCAG AACTGCGAGC ATATCCTTGC TGCTGAAACC
    AGCCATGGGA AATACCCACT AAGTGAGAAC AAGGAAGTGG TCTCAGATAC CGAAGCTCAG
    CAGTCCTTCC CATCACCGTC TAGTTCATAT GCTTCCAATT TTGATCTTTC CTTTGGTCTT
    CATGGATTTG AGAAGGAACA AAGTCTCCTG AAGAAAAGAA GCTCCAGCCT GGATGCCTTG
    GATGCCGACA GCGAAGGGGA AGGGCATTCT GAGCGGTCAC ACATCTGCTA CACGCCAGCA
    TCTCAGAGTT CCTCGAGGAC TGGGATTCCC AGTGGGGATG AGCTGGACTC CTTCGAGACC
    GCCACCAAAC CTGATTTTAG TATCTCCAGG ACAGAGTCGC TTTCTTTATC AAGTAGCCTG
    CAGTTGAAGG AATCATTGCT TCCTGGAGTC CGCTCACGTT CTTATTCCTG CTCATCACCC
    AAAATTTCTT TAGGAAAAAC TCGATTGGTG CGTGATTTCA CAGTGTGCAA TTCTGGTGAA
    GAGCAAAGAG CCTTCGGCTT CCCGGAGCCC GCCAGGGAGA GGAGGATTCA AGAGGAAGAA
    TGGGATAAAT ACATCATACC TGCCAAGTCA GAGTCTGAGA AATACAAAGT GAGCCGGACC
    TTCAGCTTCC TCATGAACAG GATGACAAGC CCTCGGAACA AATCTAAGAC GAAAAGCAAG
    GATGCCAAAG ACAAAGAGAA ACCGAGCCGA CATCAGTTTG TCCCCGGAAC CTTCTCTGGG
    GTTCTCCAGT GTTCCGTTTG TGATAAAACG CTCCTGGGGA AGGAGTCATC CCAGTGTTCC
    AACTGTAATG CAAACGTCCA CAAGGGTTGT AAGGACGCTG CACCTCCGTG TACCAAGAAA
    TTCCAGGAGA AATATAACAA GAACAAACCG CAGACCATCC TTGGAAACTC TTCATTTCGA
    GACATCCCGC AACCCAGTCT CTCCTTGCAC CCTTCTTCCT CCATGCCTAT CGGATTGCCC
    ACTGGGAGAA AAGAAGCTTC TGCCCAGGCC CATCCCTTGT CCAGAAGTGT TCCAGGCACT
    GCCTTGGAAA GCTTCAGAAG ATCGGGGCTA TCCCTGGAGT CTGAGAGTGA CGGGAACAGC
    TGGAGAAGCA GGTCTCATTC CGATGAGCTG TTACAGTCCA TGGGCTCTTC TCCCTCCACG
    GAATCCTTCT TAATGGAAGA TGTCGTGGAC TCTTCTCTAT GGAGTGACCT CAGCAGTGAC
    GCCCAGGAGT TGGAAGCAGA ATCCTGGAGT CTGGTGGTGG ACCCCTCCTT TTGCAATAAG
    CAGGAGAAAG ATGTCATCAA AAGACAGGAT GTCATTTTCG AGCTTATGCA AACAGAGATG
    CATCATATCC AGACCCTGCT CATCATGTCT GAGATCTTCA GGAAAGGAAT GAAGGAGGAG
    CTGCAGCTTG ATCACAGCAC CGTGGATAAA ATCTTTCCCT GCTTAGATGA GTTACTTGAA
    ATCCACAGGC ATTTCTTTTA TAGTATGAAG GAGCGAAGGC AGGAGTCCTG TGCTGGTGGT
    GACAGGAATT TTGTCATCAG CCGAATTGGA GATATTCTAG TACAGCAGTT TTCAGAAGAA
    AATGCAAATC AAATGAAGAA AATATATGGA GAATTCTGTA GCCATCACAA AGAAGCTGTT
    AGCCTCTATA AAGAACTCCA ACAGAATAAG AAGTTTCAGA ATTTTATTAA GCTTCGAAAT
    AGTAATCTTT TGGCTCGACG CCGAGGAATC CCAGAATGCA TTCTGCTAGT CACTCAGCGC
    ATCACAAAAT ACCCTGTCCT GGTGGAACGG ATTTTGCAGT ACACAAAGGA AAGAAGTGAA
    GAACATCAAG ACTTGTGCAA AGCTCTGTGC TTAATTAAAG ACATGATTGC AGCAGTGGAT
    TTAAAAGTCA GCGAGTATGA GAAAAACCAA AAATGGCTTG AGATCCTAAA TAAGATCGAA
    AACAAAACCT ACACAAAGCT CAAAAATGGC CACGTGTTTA GGAAGCAAGC ACTGATAAGT
    AAAGAAAGGA CTCTCTTATA TGATGGCCTT GTTTATTGGA AAACTGCTAC GGGTCGTTTC
    AAAGATATCC TGGCTCTACT TCTAACTGAT GTGCTGCTCT TTTTACAAGA AAAAGACCAG
    AAATACATCT TTGCAGCTGT TGATCAGAAG CCATCGGTTA TTTCCCTTCA GAAGCTCATT
    GCTAGAGAAG TCGCCAACGA GGAAAGAGGA ATGTTCCTGA TCAGTGCATC CTCTGCTGGT
    CCTGAGATGT ACGAAATTCA CACCAACTCC AAGGAGGAAC GAAATAACTG GATGAGACGA
    ATCCAACAGG CCGTGGAAAG TTGCCCAGAA GAAGAAGGGG GAAGGACAAG TGAATCTGAT
    GAAGAGAGGC GGAAAGCTGA AGCAAGAGTG GCCAAAATCC AGCAATGTCA AGAAATACTC
    AGTAACCAAG ACCAACAGAT TTGCGCATAT TTGGAGGAGA AGCTGCATAT CTACGCTGAA
    CTTGGAGAAC TGAGTGGATT CAAGGATGTC CATCTAGAGC CCCACCTCCT CATCAAACCT
    GACCCAGGGG AGCCTCCCCA GGCAGCCTCA TTACTAGCAG CAGCCCTGAG AGAAGCGGAG
    AGCCTGCAGG TTGCCGTGAA GGCCTCACAA GCGGGTGATG CTCGCCCATC ACCTGAGGAG
    GGTTCTGCAG AACCTGCGGT TGCAGATGCA CAGGGTCCTC CCGAGGAGCC AGGATCATCT
    CCAGCCTCAC TAGTCACAGA AGGGACAGGA GGAAGAGGCT GTTGGGATGT GGATCCTGGC
    CCCCAGGGTG TGGGAACCGA CTTGGCGGTC TCTGATGCAG GGGAGAAGAG TCTTAAAAAT
    CTGTGCTGCA GCCGGCAAAG TCCCTGGGGT GTCAGTCACC ATCACCAGCT GCAGCCCTGC
    ACAGGGACCA CAAGGGGGAG GGCCAGCTCC ACGAAAAGTA GGACACTACA CAGCTCAGGC
    TTGTATCAGG CTTCGTGGTT CTTGGGAGTG GAATATAGAA GTTTCACGGT TTCTTCACAG
    TCAGAGATTA TACAAGCGAT CCAGAATTTA ACCCGACTCC TCTATAGCCT TCAGGCCGCG
    CTGACCATCC AGGACAGCCA CATCCAGGTG CACAGGCTGC TGCTGCAGCA GGCTGGCCTG
    CCGGCGTCCC AAGAGGAGCA CGGCGAGCTG GCGAGGCAGC GGGAGGAGCT GGCCGCCCTG
    CCCGGGCTCC AGTGGCAGCT GCAGCAGGAG CGGCGGCGCG GCCAGCGCAC CTGGGAGCGG
    CAGCAGCGCG AGCAGGAGGC CAGGGCCTGC CGGCTGCGGG AGCGCGAGCA GGGCTGCCGC
    GGCCGCGAGG AGCTGCTGCT CAGGGCCCGC GGGGAGCTGG ACCGGCAGCT GCGGGAGCAC
    CGGCAGGACC TGGAGCGGCT GCGGGAGGGC CAGCGCCTGG TGGAGAGTGC GAGGGCCCGC
    ACGCGCGCCC AGCACAGCCT GCTGCGTGGC CCGAGGCCCG GGCGCCAGAG CAGCCTGCCC
    GCGGCCTTGC CTCCGGGCAG CGGCGAGGTG ATGGAACTTA ATCGATCCGA GAGCTTGTGT
    CATGAAAATT CATTCTTCAT TAATGAAGCT TTAGTACAAA TGTCACTTAA CACTTACAAC
    AAATCAACTC CATCAGGTGT CCACCAGGAA GCCGCTTATC CCCAGAATAT AGCTAATTCG
    GACTTGGTAA GGACTAGTGA AAGTCCCGTA GACCTCCAGT TGGACATTTC TCAGCCCTTG
    GATGTCAACC ACGAACTGTG GACAGCCGCT GGGTCCTGTC ATCAGATACC CCCTCTCCAC
    GAAAGCAGCA AGGATTCTTG TAAAAATGGA AGAATTTACA TCAGTCAGCT ATGTTTCCAG
    AGCCATTCGT CAGAAATGTT TTGGCGACCA GACTTGACCA TCTCAGCTTG GGATAGAGAG
    CTTCCGAATC TGCAGAACTT CCAACCGCTG TTGGTTGCAT TATGTAGATG GTTGCGTTAC
    GTCCGTACAT CCCCGGGAGA TCTTGCCGTC ATCCTCGAGT CCCCTGAGCA GGTTTTTCAG
    CTAGAAAGAA AATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35386
    Clone ID Related Accession (Same CDS sequence) XM_014109079.1
    Accession Version XM_014109079.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4314bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product rho guanine nucleotide exchange factor 28 isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCTTAAAT CAAACTCCCT TCAAGTGAAA GCAGATCACT CTTTCGATGT CTTCAAAAAA 
    CCTAAGCATC CCCCAGCGTT CTTTGCTGCA GGCGGGCTCT CTGATGTGCT GAATGGCGGC
    GATGAAGTTT ACGCCAACTG TATGGTGATT GACCAGGTTG GTGATTTGGA TATTAACTAC
    ATCAATTTAG AAGGAATCTC TACAGACGCC TGTGCTGAAT CCATGGGTTC TACTCTGGGG
    CCTCAGAACT GCGAGCATAT CCTTGCTGCT GAAACCAGCC ATGGGAAATA CCCACTAAGT
    GAGAACAAGG AAGTGGTCTC AGATACCGAA GCTCAGCAGT CCTTCCCATC ACCGTCTAGT
    TCATATGCTT CCAATTTTGA TCTTTCCTTT GGTCTTCATG GATTTGAGAA GGAACAAAGT
    CTCCTGAAGA AAAGAAGCTC CAGCCTGGAT GCCTTGGATG CCGACAGCGA AGGGGAAGGG
    CATTCTGAGC GGTCACACAT CTGCTACACG CCAGCATCTC AGAGTTCCTC GAGGACTGGG
    ATTCCCAGTG GGGATGAGCT GGACTCCTTC GAGACCGCCA CCAAACCTGA TTTTAGTATC
    TCCAGGACAG AGTCGCTTTC TTTATCAAGT AGCCTGCAGT TGAAGGAATC ATTGCTTCCT
    GGAGTCCGCT CACGTTCTTA TTCCTGCTCA TCACCCAAAA TTTCTTTAGG AAAAACTCGA
    TTGGTGCGTG ATTTCACAGT GTGCAATTCT GGTGAAGAGC AAAGAGCCTT CGGCTTCCCG
    GAGCCCGCCA GGGAGAGGAG GATTCAAGAG GAAGAATGGG ATAAATACAT CATACCTGCC
    AAGTCAGAGT CTGAGAAATA CAAAGTGAGC CGGACCTTCA GCTTCCTCAT GAACAGGATG
    ACAAGCCCTC GGAACAAATC TAAGACGAAA AGCAAGGATG CCAAAGACAA AGAGAAACCG
    AGCCGACATC AGTTTGTCCC CGGAACCTTC TCTGGGGTTC TCCAGTGTTC CGTTTGTGAT
    AAAACGCTCC TGGGGAAGGA GTCATCCCAG TGTTCCAACT GTAATGCAAA CGTCCACAAG
    GGTTGTAAGG ACGCTGCACC TCCGTGTACC AAGAAATTCC AGGAGAAATA TAACAAGAAC
    AAACCGCAGA CCATCCTTGG AAACTCTTCA TTTCGAGACA TCCCGCAACC CAGTCTCTCC
    TTGCACCCTT CTTCCTCCAT GCCTATCGGA TTGCCCACTG GGAGAAAAGA AGCTTCTGCC
    CAGGCCCATC CCTTGTCCAG AAGTGTTCCA GGCACTGCCT TGGAAAGCTT CAGAAGATCG
    GGGCTATCCC TGGAGTCTGA GAGTGACGGG AACAGCTGGA GAAGCAGGTC TCATTCCGAT
    GAGCTGTTAC AGTCCATGGG CTCTTCTCCC TCCACGGAAT CCTTCTTAAT GGAAGATGTC
    GTGGACTCTT CTCTATGGAG TGACCTCAGC AGTGACGCCC AGGAGTTGGA AGCAGAATCC
    TGGAGTCTGG TGGTGGACCC CTCCTTTTGC AATAAGCAGG AGAAAGATGT CATCAAAAGA
    CAGGATGTCA TTTTCGAGCT TATGCAAACA GAGATGCATC ATATCCAGAC CCTGCTCATC
    ATGTCTGAGA TCTTCAGGAA AGGAATGAAG GAGGAGCTGC AGCTTGATCA CAGCACCGTG
    GATAAAATCT TTCCCTGCTT AGATGAGTTA CTTGAAATCC ACAGGCATTT CTTTTATAGT
    ATGAAGGAGC GAAGGCAGGA GTCCTGTGCT GGTGGTGACA GGAATTTTGT CATCAGCCGA
    ATTGGAGATA TTCTAGTACA GCAGTTTTCA GAAGAAAATG CAAATCAAAT GAAGAAAATA
    TATGGAGAAT TCTGTAGCCA TCACAAAGAA GCTGTTAGCC TCTATAAAGA ACTCCAACAG
    AATAAGAAGT TTCAGAATTT TATTAAGCTT CGAAATAGTA ATCTTTTGGC TCGACGCCGA
    GGAATCCCAG AATGCATTCT GCTAGTCACT CAGCGCATCA CAAAATACCC TGTCCTGGTG
    GAACGGATTT TGCAGTACAC AAAGGAAAGA AGTGAAGAAC ATCAAGACTT GTGCAAAGCT
    CTGTGCTTAA TTAAAGACAT GATTGCAGCA GTGGATTTAA AAGTCAGCGA GTATGAGAAA
    AACCAAAAAT GGCTTGAGAT CCTAAATAAG ATCGAAAACA AAACCTACAC AAAGCTCAAA
    AATGGCCACG TGTTTAGGAA GCAAGCACTG ATAAGTAAAG AAAGGACTCT CTTATATGAT
    GGCCTTGTTT ATTGGAAAAC TGCTACGGGT CGTTTCAAAG ATATCCTGGC TCTACTTCTA
    ACTGATGTGC TGCTCTTTTT ACAAGAAAAA GACCAGAAAT ACATCTTTGC AGCTGTTGAT
    CAGAAGCCAT CGGTTATTTC CCTTCAGAAG CTCATTGCTA GAGAAGTCGC CAACGAGGAA
    AGAGGAATGT TCCTGATCAG TGCATCCTCT GCTGGTCCTG AGATGTACGA AATTCACACC
    AACTCCAAGG AGGAACGAAA TAACTGGATG AGACGAATCC AACAGGCCGT GGAAAGTTGC
    CCAGAAGAAG AAGGGGGAAG GACAAGTGAA TCTGATGAAG AGAGGCGGAA AGCTGAAGCA
    AGAGTGGCCA AAATCCAGCA ATGTCAAGAA ATACTCAGTA ACCAAGACCA ACAGATTTGC
    GCATATTTGG AGGAGAAGCT GCATATCTAC GCTGAACTTG GAGAACTGAG TGGATTCAAG
    GATGTCCATC TAGAGCCCCA CCTCCTCATC AAACCTGACC CAGGGGAGCC TCCCCAGGCA
    GCCTCATTAC TAGCAGCAGC CCTGAGAGAA GCGGAGAGCC TGCAGGTTGC CGTGAAGGCC
    TCACAAGCGG GTGATGCTCG CCCATCACCT GAGGAGGGTT CTGCAGAACC TGCGGTTGCA
    GATGCACAGG GTCCTCCCGA GGAGCCAGGA TCATCTCCAG CCTCACTAGT CACAGAAGGG
    ACAGGAGGAA GAGGCTGTTG GGATGTGGAT CCTGGCCCCC AGGGTGTGGG AACCGACTTG
    GCGGTCTCTG ATGCAGGGGA GAAGAGTCTT AAAAATCTGT GCTGCAGCCG GCAAAGTCCC
    TGGGGTGTCA GTCACCATCA CCAGCTGCAG CCCTGCACAG GGACCACAAG GGGGAGGGCC
    AGCTCCACGA AAAGTAGGAC ACTACACAGC TCAGGCTTGT ATCAGGCTTC GTGGTTCTTG
    GGAGTGGAAT ATAGAAGTTT CACGGTTTCT TCACAGTCAG AGATTATACA AGCGATCCAG
    AATTTAACCC GACTCCTCTA TAGCCTTCAG GCCGCGCTGA CCATCCAGGA CAGCCACATC
    CAGGTGCACA GGCTGCTGCT GCAGCAGGCT GGCCTGCCGG CGTCCCAAGA GGAGCACGGC
    GAGCTGGCGA GGCAGCGGGA GGAGCTGGCC GCCCTGCCCG GGCTCCAGTG GCAGCTGCAG
    CAGGAGCGGC GGCGCGGCCA GCGCACCTGG GAGCGGCAGC AGCGCGAGCA GGAGGCCAGG
    GCCTGCCGGC TGCGGGAGCG CGAGCAGGGC TGCCGCGGCC GCGAGGAGCT GCTGCTCAGG
    GCCCGCGGGG AGCTGGACCG GCAGCTGCGG GAGCACCGGC AGGACCTGGA GCGGCTGCGG
    GAGGGCCAGC GCCTGGTGGA GAGTGCGAGG GCCCGCACGC GCGCCCAGCA CAGCCTGCTG
    CGTGGCCCGA GGCCCGGGCG CCAGAGCAGC CTGCCCGCGG CCTTGCCTCC GGGCAGCGGC
    GAGGTGATGG AACTTAATCG ATCCGAGAGC TTGTGTCATG AAAATTCATT CTTCATTAAT
    GAAGCTTTAG TACAAATGTC ACTTAACACT TACAACAAAT CAACTCCATC AGGTGTCCAC
    CAGGAAGCCG CTTATCCCCA GAATATAGCT AATTCGGACT TGGTAAGGAC TAGTGAAAGT
    CCCGTAGACC TCCAGTTGGA CATTTCTCAG CCCTTGGATG TCAACCACGA ACTGTGGACA
    GCCGCTGGGT CCTGTCATCA GATACCCCCT CTCCACGAAA GCAGCAAGGA TTCTTGTAAA
    AATGGAAGAA TTTACATCAG TCAGCTATGT TTCCAGAGCC ATTCGTCAGA AATGTTTTGG
    CGACCAGACT TGACCATCTC AGCTTGGGAT AGAGAGCTTC CGAATCTGCA GAACTTCCAA
    CCGCTGTTGG TTGCATTATG TAGATGGTTG CGTTACGTCC GTACATCCCC GGGAGATCTT
    GCCGTCATCC TCGAGTCCCC TGAGCAGGTT TTTCAGCTAG AAAGAAAATG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013964554.1
    CDS16..4329
    Translation

    Target ORF information:

    RefSeq Version XM_014109079.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 28 (ARHGEF28), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109079.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCTTAAAT CAAACTCCCT TCAAGTGAAA GCAGATCACT CTTTCGATGT CTTCAAAAAA 
    CCTAAGCATC CCCCAGCGTT CTTTGCTGCA GGCGGGCTCT CTGATGTGCT GAATGGCGGC
    GATGAAGTTT ACGCCAACTG TATGGTGATT GACCAGGTTG GTGATTTGGA TATTAACTAC
    ATCAATTTAG AAGGAATCTC TACAGACGCC TGTGCTGAAT CCATGGGTTC TACTCTGGGG
    CCTCAGAACT GCGAGCATAT CCTTGCTGCT GAAACCAGCC ATGGGAAATA CCCACTAAGT
    GAGAACAAGG AAGTGGTCTC AGATACCGAA GCTCAGCAGT CCTTCCCATC ACCGTCTAGT
    TCATATGCTT CCAATTTTGA TCTTTCCTTT GGTCTTCATG GATTTGAGAA GGAACAAAGT
    CTCCTGAAGA AAAGAAGCTC CAGCCTGGAT GCCTTGGATG CCGACAGCGA AGGGGAAGGG
    CATTCTGAGC GGTCACACAT CTGCTACACG CCAGCATCTC AGAGTTCCTC GAGGACTGGG
    ATTCCCAGTG GGGATGAGCT GGACTCCTTC GAGACCGCCA CCAAACCTGA TTTTAGTATC
    TCCAGGACAG AGTCGCTTTC TTTATCAAGT AGCCTGCAGT TGAAGGAATC ATTGCTTCCT
    GGAGTCCGCT CACGTTCTTA TTCCTGCTCA TCACCCAAAA TTTCTTTAGG AAAAACTCGA
    TTGGTGCGTG ATTTCACAGT GTGCAATTCT GGTGAAGAGC AAAGAGCCTT CGGCTTCCCG
    GAGCCCGCCA GGGAGAGGAG GATTCAAGAG GAAGAATGGG ATAAATACAT CATACCTGCC
    AAGTCAGAGT CTGAGAAATA CAAAGTGAGC CGGACCTTCA GCTTCCTCAT GAACAGGATG
    ACAAGCCCTC GGAACAAATC TAAGACGAAA AGCAAGGATG CCAAAGACAA AGAGAAACCG
    AGCCGACATC AGTTTGTCCC CGGAACCTTC TCTGGGGTTC TCCAGTGTTC CGTTTGTGAT
    AAAACGCTCC TGGGGAAGGA GTCATCCCAG TGTTCCAACT GTAATGCAAA CGTCCACAAG
    GGTTGTAAGG ACGCTGCACC TCCGTGTACC AAGAAATTCC AGGAGAAATA TAACAAGAAC
    AAACCGCAGA CCATCCTTGG AAACTCTTCA TTTCGAGACA TCCCGCAACC CAGTCTCTCC
    TTGCACCCTT CTTCCTCCAT GCCTATCGGA TTGCCCACTG GGAGAAAAGA AGCTTCTGCC
    CAGGCCCATC CCTTGTCCAG AAGTGTTCCA GGCACTGCCT TGGAAAGCTT CAGAAGATCG
    GGGCTATCCC TGGAGTCTGA GAGTGACGGG AACAGCTGGA GAAGCAGGTC TCATTCCGAT
    GAGCTGTTAC AGTCCATGGG CTCTTCTCCC TCCACGGAAT CCTTCTTAAT GGAAGATGTC
    GTGGACTCTT CTCTATGGAG TGACCTCAGC AGTGACGCCC AGGAGTTGGA AGCAGAATCC
    TGGAGTCTGG TGGTGGACCC CTCCTTTTGC AATAAGCAGG AGAAAGATGT CATCAAAAGA
    CAGGATGTCA TTTTCGAGCT TATGCAAACA GAGATGCATC ATATCCAGAC CCTGCTCATC
    ATGTCTGAGA TCTTCAGGAA AGGAATGAAG GAGGAGCTGC AGCTTGATCA CAGCACCGTG
    GATAAAATCT TTCCCTGCTT AGATGAGTTA CTTGAAATCC ACAGGCATTT CTTTTATAGT
    ATGAAGGAGC GAAGGCAGGA GTCCTGTGCT GGTGGTGACA GGAATTTTGT CATCAGCCGA
    ATTGGAGATA TTCTAGTACA GCAGTTTTCA GAAGAAAATG CAAATCAAAT GAAGAAAATA
    TATGGAGAAT TCTGTAGCCA TCACAAAGAA GCTGTTAGCC TCTATAAAGA ACTCCAACAG
    AATAAGAAGT TTCAGAATTT TATTAAGCTT CGAAATAGTA ATCTTTTGGC TCGACGCCGA
    GGAATCCCAG AATGCATTCT GCTAGTCACT CAGCGCATCA CAAAATACCC TGTCCTGGTG
    GAACGGATTT TGCAGTACAC AAAGGAAAGA AGTGAAGAAC ATCAAGACTT GTGCAAAGCT
    CTGTGCTTAA TTAAAGACAT GATTGCAGCA GTGGATTTAA AAGTCAGCGA GTATGAGAAA
    AACCAAAAAT GGCTTGAGAT CCTAAATAAG ATCGAAAACA AAACCTACAC AAAGCTCAAA
    AATGGCCACG TGTTTAGGAA GCAAGCACTG ATAAGTAAAG AAAGGACTCT CTTATATGAT
    GGCCTTGTTT ATTGGAAAAC TGCTACGGGT CGTTTCAAAG ATATCCTGGC TCTACTTCTA
    ACTGATGTGC TGCTCTTTTT ACAAGAAAAA GACCAGAAAT ACATCTTTGC AGCTGTTGAT
    CAGAAGCCAT CGGTTATTTC CCTTCAGAAG CTCATTGCTA GAGAAGTCGC CAACGAGGAA
    AGAGGAATGT TCCTGATCAG TGCATCCTCT GCTGGTCCTG AGATGTACGA AATTCACACC
    AACTCCAAGG AGGAACGAAA TAACTGGATG AGACGAATCC AACAGGCCGT GGAAAGTTGC
    CCAGAAGAAG AAGGGGGAAG GACAAGTGAA TCTGATGAAG AGAGGCGGAA AGCTGAAGCA
    AGAGTGGCCA AAATCCAGCA ATGTCAAGAA ATACTCAGTA ACCAAGACCA ACAGATTTGC
    GCATATTTGG AGGAGAAGCT GCATATCTAC GCTGAACTTG GAGAACTGAG TGGATTCAAG
    GATGTCCATC TAGAGCCCCA CCTCCTCATC AAACCTGACC CAGGGGAGCC TCCCCAGGCA
    GCCTCATTAC TAGCAGCAGC CCTGAGAGAA GCGGAGAGCC TGCAGGTTGC CGTGAAGGCC
    TCACAAGCGG GTGATGCTCG CCCATCACCT GAGGAGGGTT CTGCAGAACC TGCGGTTGCA
    GATGCACAGG GTCCTCCCGA GGAGCCAGGA TCATCTCCAG CCTCACTAGT CACAGAAGGG
    ACAGGAGGAA GAGGCTGTTG GGATGTGGAT CCTGGCCCCC AGGGTGTGGG AACCGACTTG
    GCGGTCTCTG ATGCAGGGGA GAAGAGTCTT AAAAATCTGT GCTGCAGCCG GCAAAGTCCC
    TGGGGTGTCA GTCACCATCA CCAGCTGCAG CCCTGCACAG GGACCACAAG GGGGAGGGCC
    AGCTCCACGA AAAGTAGGAC ACTACACAGC TCAGGCTTGT ATCAGGCTTC GTGGTTCTTG
    GGAGTGGAAT ATAGAAGTTT CACGGTTTCT TCACAGTCAG AGATTATACA AGCGATCCAG
    AATTTAACCC GACTCCTCTA TAGCCTTCAG GCCGCGCTGA CCATCCAGGA CAGCCACATC
    CAGGTGCACA GGCTGCTGCT GCAGCAGGCT GGCCTGCCGG CGTCCCAAGA GGAGCACGGC
    GAGCTGGCGA GGCAGCGGGA GGAGCTGGCC GCCCTGCCCG GGCTCCAGTG GCAGCTGCAG
    CAGGAGCGGC GGCGCGGCCA GCGCACCTGG GAGCGGCAGC AGCGCGAGCA GGAGGCCAGG
    GCCTGCCGGC TGCGGGAGCG CGAGCAGGGC TGCCGCGGCC GCGAGGAGCT GCTGCTCAGG
    GCCCGCGGGG AGCTGGACCG GCAGCTGCGG GAGCACCGGC AGGACCTGGA GCGGCTGCGG
    GAGGGCCAGC GCCTGGTGGA GAGTGCGAGG GCCCGCACGC GCGCCCAGCA CAGCCTGCTG
    CGTGGCCCGA GGCCCGGGCG CCAGAGCAGC CTGCCCGCGG CCTTGCCTCC GGGCAGCGGC
    GAGGTGATGG AACTTAATCG ATCCGAGAGC TTGTGTCATG AAAATTCATT CTTCATTAAT
    GAAGCTTTAG TACAAATGTC ACTTAACACT TACAACAAAT CAACTCCATC AGGTGTCCAC
    CAGGAAGCCG CTTATCCCCA GAATATAGCT AATTCGGACT TGGTAAGGAC TAGTGAAAGT
    CCCGTAGACC TCCAGTTGGA CATTTCTCAG CCCTTGGATG TCAACCACGA ACTGTGGACA
    GCCGCTGGGT CCTGTCATCA GATACCCCCT CTCCACGAAA GCAGCAAGGA TTCTTGTAAA
    AATGGAAGAA TTTACATCAG TCAGCTATGT TTCCAGAGCC ATTCGTCAGA AATGTTTTGG
    CGACCAGACT TGACCATCTC AGCTTGGGAT AGAGAGCTTC CGAATCTGCA GAACTTCCAA
    CCGCTGTTGG TTGCATTATG TAGATGGTTG CGTTACGTCC GTACATCCCC GGGAGATCTT
    GCCGTCATCC TCGAGTCCCC TGAGCAGGTT TTTCAGCTAG AAAGAAAATG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35387
    Clone ID Related Accession (Same CDS sequence) XM_014109081.2 , XM_022410519.1 , XM_014109081.1
    Accession Version XM_014109081.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4173bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rho guanine nucleotide exchange factor 28 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109081.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGTGATTG ACCAGGTTGG TGATTTGGAT ATTAACTACA TCAATTTAGA AGGAATCTCT 
    ACAGACGCCT GTGCTGAATC CATGGGTTCT ACTCTGGGGC CTCAGAACTG CGAGCATATC
    CTTGCTGCTG AAACCAGCCA TGGGAAATAC CCACTAAGTG AGAACAAGGA AGTGGTCTCA
    GATACCGAAG CTCAGCAGTC CTTCCCATCA CCGTCTAGTT CATATGCTTC CAATTTTGAT
    CTTTCCTTTG GTCTTCATGG ATTTGAGAAG GAACAAAGTC TCCTGAAGAA AAGAAGCTCC
    AGCCTGGATG CCTTGGATGC CGACAGCGAA GGGGAAGGGC ATTCTGAGCG GTCACACATC
    TGCTACACGC CAGCATCTCA GAGTTCCTCG AGGACTGGGA TTCCCAGTGG GGATGAGCTG
    GACTCCTTCG AGACCGCCAC CAAACCTGAT TTTAGTATCT CCAGGACAGA GTCGCTTTCT
    TTATCAAGTA GCCTGCAGTT GAAGGAATCA TTGCTTCCTG GAGTCCGCTC ACGTTCTTAT
    TCCTGCTCAT CACCCAAAAT TTCTTTAGGA AAAACTCGAT TGGTGCGTGA TTTCACAGTG
    TGCAATTCTG GTGAAGAGCA AAGAGCCTTC GGCTTCCCGG AGCCCGCCAG GGAGAGGAGG
    ATTCAAGAGG AAGAATGGGA TAAATACATC ATACCTGCCA AGTCAGAGTC TGAGAAATAC
    AAAGTGAGCC GGACCTTCAG CTTCCTCATG AACAGGATGA CAAGCCCTCG GAACAAATCT
    AAGACGAAAA GCAAGGATGC CAAAGACAAA GAGAAACCGA GCCGACATCA GTTTGTCCCC
    GGAACCTTCT CTGGGGTTCT CCAGTGTTCC GTTTGTGATA AAACGCTCCT GGGGAAGGAG
    TCATCCCAGT GTTCCAACTG TAATGCAAAC GTCCACAAGG GTTGTAAGGA CGCTGCACCT
    CCGTGTACCA AGAAATTCCA GGAGAAATAT AACAAGAACA AACCGCAGAC CATCCTTGGA
    AACTCTTCAT TTCGAGACAT CCCGCAACCC AGTCTCTCCT TGCACCCTTC TTCCTCCATG
    CCTATCGGAT TGCCCACTGG GAGAAAAGAA GCTTCTGCCC AGGCCCATCC CTTGTCCAGA
    AGTGTTCCAG GCACTGCCTT GGAAAGCTTC AGAAGATCGG GGCTATCCCT GGAGTCTGAG
    AGTGACGGGA ACAGCTGGAG AAGCAGGTCT CATTCCGATG AGCTGTTACA GTCCATGGGC
    TCTTCTCCCT CCACGGAATC CTTCTTAATG GAAGATGTCG TGGACTCTTC TCTATGGAGT
    GACCTCAGCA GTGACGCCCA GGAGTTGGAA GCAGAATCCT GGAGTCTGGT GGTGGACCCC
    TCCTTTTGCA ATAAGCAGGA GAAAGATGTC ATCAAAAGAC AGGATGTCAT TTTCGAGCTT
    ATGCAAACAG AGATGCATCA TATCCAGACC CTGCTCATCA TGTCTGAGAT CTTCAGGAAA
    GGAATGAAGG AGGAGCTGCA GCTTGATCAC AGCACCGTGG ATAAAATCTT TCCCTGCTTA
    GATGAGTTAC TTGAAATCCA CAGGCATTTC TTTTATAGTA TGAAGGAGCG AAGGCAGGAG
    TCCTGTGCTG GTGGTGACAG GAATTTTGTC ATCAGCCGAA TTGGAGATAT TCTAGTACAG
    CAGTTTTCAG AAGAAAATGC AAATCAAATG AAGAAAATAT ATGGAGAATT CTGTAGCCAT
    CACAAAGAAG CTGTTAGCCT CTATAAAGAA CTCCAACAGA ATAAGAAGTT TCAGAATTTT
    ATTAAGCTTC GAAATAGTAA TCTTTTGGCT CGACGCCGAG GAATCCCAGA ATGCATTCTG
    CTAGTCACTC AGCGCATCAC AAAATACCCT GTCCTGGTGG AACGGATTTT GCAGTACACA
    AAGGAAAGAA GTGAAGAACA TCAAGACTTG TGCAAAGCTC TGTGCTTAAT TAAAGACATG
    ATTGCAGCAG TGGATTTAAA AGTCAGCGAG TATGAGAAAA ACCAAAAATG GCTTGAGATC
    CTAAATAAGA TCGAAAACAA AACCTACACA AAGCTCAAAA ATGGCCACGT GTTTAGGAAG
    CAAGCACTGA TAAGTAAAGA AAGGACTCTC TTATATGATG GCCTTGTTTA TTGGAAAACT
    GCTACGGGTC GTTTCAAAGA TATCCTGGCT CTACTTCTAA CTGATGTGCT GCTCTTTTTA
    CAAGAAAAAG ACCAGAAATA CATCTTTGCA GCTGTTGATC AGAAGCCATC GGTTATTTCC
    CTTCAGAAGC TCATTGCTAG AGAAGTCGCC AACGAGGAAA GAGGAATGTT CCTGATCAGT
    GCATCCTCTG CTGGTCCTGA GATGTACGAA ATTCACACCA ACTCCAAGGA GGAACGAAAT
    AACTGGATGA GACGAATCCA ACAGGCCGTG GAAAGTTGCC CAGAAGAAGA AGGGGGAAGG
    ACAAGTGAAT CTGATGAAGA GAGGCGGAAA GCTGAAGCAA GAGTGGCCAA AATCCAGCAA
    TGTCAAGAAA TACTCAGTAA CCAAGACCAA CAGATTTGCG CATATTTGGA GGAGAAGCTG
    CATATCTACG CTGAACTTGG AGAACTGAGT GGATTCAAGG ATGTCCATCT AGAGCCCCAC
    CTCCTCATCA AACCTGACCC AGGGGAGCCT CCCCAGGCAG CCTCATTACT AGCAGCAGCC
    CTGAGAGAAG CGGAGAGCCT GCAGGTTGCC GTGAAGGCCT CACAAGCGGG TGATGCTCGC
    CCATCACCTG AGGAGGGTTC TGCAGAACCT GCGGTTGCAG ATGCACAGGG TCCTCCCGAG
    GAGCCAGGAT CATCTCCAGC CTCACTAGTC ACAGAAGGGA CAGGAGGAAG AGGCTGTTGG
    GATGTGGATC CTGGCCCCCA GGGTGTGGGA ACCGACTTGG CGGTCTCTGA TGCAGGGGAG
    AAGAGTCTTA AAAATCTGTG CTGCAGCCGG CAAAGTCCCT GGGGTGTCAG TCACCATCAC
    CAGCTGCAGC CCTGCACAGG GACCACAAGG GGGAGGGCCA GCTCCACGAA AAGTAGGACA
    CTACACAGCT CAGGCTTGTA TCAGGCTTCG TGGTTCTTGG GAGTGGAATA TAGAAGTTTC
    ACGGTTTCTT CACAGTCAGA GATTATACAA GCGATCCAGA ATTTAACCCG ACTCCTCTAT
    AGCCTTCAGG CCGCGCTGAC CATCCAGGAC AGCCACATCC AGGTGCACAG GCTGCTGCTG
    CAGCAGGCTG GCCTGCCGGC GTCCCAAGAG GAGCACGGCG AGCTGGCGAG GCAGCGGGAG
    GAGCTGGCCG CCCTGCCCGG GCTCCAGTGG CAGCTGCAGC AGGAGCGGCG GCGCGGCCAG
    CGCACCTGGG AGCGGCAGCA GCGCGAGCAG GAGGCCAGGG CCTGCCGGCT GCGGGAGCGC
    GAGCAGGGCT GCCGCGGCCG CGAGGAGCTG CTGCTCAGGG CCCGCGGGGA GCTGGACCGG
    CAGCTGCGGG AGCACCGGCA GGACCTGGAG CGGCTGCGGG AGGGCCAGCG CCTGGTGGAG
    AGTGCGAGGG CCCGCACGCG CGCCCAGCAC AGCCTGCTGC GTGGCCCGAG GCCCGGGCGC
    CAGAGCAGCC TGCCCGCGGC CTTGCCTCCG GGCAGCGGCG AGGTGATGGA ACTTAATCGA
    TCCGAGAGCT TGTGTCATGA AAATTCATTC TTCATTAATG AAGCTTTAGT ACAAATGTCA
    CTTAACACTT ACAACAAATC AACTCCATCA GGTGTCCACC AGGAAGCCGC TTATCCCCAG
    AATATAGCTA ATTCGGACTT GGTAAGGACT AGTGAAAGTC CCGTAGACCT CCAGTTGGAC
    ATTTCTCAGC CCTTGGATGT CAACCACGAA CTGTGGACAG CCGCTGGGTC CTGTCATCAG
    ATACCCCCTC TCCACGAAAG CAGCAAGGAT TCTTGTAAAA ATGGAAGAAT TTACATCAGT
    CAGCTATGTT TCCAGAGCCA TTCGTCAGAA ATGTTTTGGC GACCAGACTT GACCATCTCA
    GCTTGGGATA GAGAGCTTCC GAATCTGCAG AACTTCCAAC CGCTGTTGGT TGCATTATGT
    AGATGGTTGC GTTACGTCCG TACATCCCCG GGAGATCTTG CCGTCATCCT CGAGTCCCCT
    GAGCAGGTTT TTCAGCTAGA AAGAAAATGT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013964556.1
    CDS599..4771
    Translation

    Target ORF information:

    RefSeq Version XM_014109081.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109081.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGTGATTG ACCAGGTTGG TGATTTGGAT ATTAACTACA TCAATTTAGA AGGAATCTCT 
    ACAGACGCCT GTGCTGAATC CATGGGTTCT ACTCTGGGGC CTCAGAACTG CGAGCATATC
    CTTGCTGCTG AAACCAGCCA TGGGAAATAC CCACTAAGTG AGAACAAGGA AGTGGTCTCA
    GATACCGAAG CTCAGCAGTC CTTCCCATCA CCGTCTAGTT CATATGCTTC CAATTTTGAT
    CTTTCCTTTG GTCTTCATGG ATTTGAGAAG GAACAAAGTC TCCTGAAGAA AAGAAGCTCC
    AGCCTGGATG CCTTGGATGC CGACAGCGAA GGGGAAGGGC ATTCTGAGCG GTCACACATC
    TGCTACACGC CAGCATCTCA GAGTTCCTCG AGGACTGGGA TTCCCAGTGG GGATGAGCTG
    GACTCCTTCG AGACCGCCAC CAAACCTGAT TTTAGTATCT CCAGGACAGA GTCGCTTTCT
    TTATCAAGTA GCCTGCAGTT GAAGGAATCA TTGCTTCCTG GAGTCCGCTC ACGTTCTTAT
    TCCTGCTCAT CACCCAAAAT TTCTTTAGGA AAAACTCGAT TGGTGCGTGA TTTCACAGTG
    TGCAATTCTG GTGAAGAGCA AAGAGCCTTC GGCTTCCCGG AGCCCGCCAG GGAGAGGAGG
    ATTCAAGAGG AAGAATGGGA TAAATACATC ATACCTGCCA AGTCAGAGTC TGAGAAATAC
    AAAGTGAGCC GGACCTTCAG CTTCCTCATG AACAGGATGA CAAGCCCTCG GAACAAATCT
    AAGACGAAAA GCAAGGATGC CAAAGACAAA GAGAAACCGA GCCGACATCA GTTTGTCCCC
    GGAACCTTCT CTGGGGTTCT CCAGTGTTCC GTTTGTGATA AAACGCTCCT GGGGAAGGAG
    TCATCCCAGT GTTCCAACTG TAATGCAAAC GTCCACAAGG GTTGTAAGGA CGCTGCACCT
    CCGTGTACCA AGAAATTCCA GGAGAAATAT AACAAGAACA AACCGCAGAC CATCCTTGGA
    AACTCTTCAT TTCGAGACAT CCCGCAACCC AGTCTCTCCT TGCACCCTTC TTCCTCCATG
    CCTATCGGAT TGCCCACTGG GAGAAAAGAA GCTTCTGCCC AGGCCCATCC CTTGTCCAGA
    AGTGTTCCAG GCACTGCCTT GGAAAGCTTC AGAAGATCGG GGCTATCCCT GGAGTCTGAG
    AGTGACGGGA ACAGCTGGAG AAGCAGGTCT CATTCCGATG AGCTGTTACA GTCCATGGGC
    TCTTCTCCCT CCACGGAATC CTTCTTAATG GAAGATGTCG TGGACTCTTC TCTATGGAGT
    GACCTCAGCA GTGACGCCCA GGAGTTGGAA GCAGAATCCT GGAGTCTGGT GGTGGACCCC
    TCCTTTTGCA ATAAGCAGGA GAAAGATGTC ATCAAAAGAC AGGATGTCAT TTTCGAGCTT
    ATGCAAACAG AGATGCATCA TATCCAGACC CTGCTCATCA TGTCTGAGAT CTTCAGGAAA
    GGAATGAAGG AGGAGCTGCA GCTTGATCAC AGCACCGTGG ATAAAATCTT TCCCTGCTTA
    GATGAGTTAC TTGAAATCCA CAGGCATTTC TTTTATAGTA TGAAGGAGCG AAGGCAGGAG
    TCCTGTGCTG GTGGTGACAG GAATTTTGTC ATCAGCCGAA TTGGAGATAT TCTAGTACAG
    CAGTTTTCAG AAGAAAATGC AAATCAAATG AAGAAAATAT ATGGAGAATT CTGTAGCCAT
    CACAAAGAAG CTGTTAGCCT CTATAAAGAA CTCCAACAGA ATAAGAAGTT TCAGAATTTT
    ATTAAGCTTC GAAATAGTAA TCTTTTGGCT CGACGCCGAG GAATCCCAGA ATGCATTCTG
    CTAGTCACTC AGCGCATCAC AAAATACCCT GTCCTGGTGG AACGGATTTT GCAGTACACA
    AAGGAAAGAA GTGAAGAACA TCAAGACTTG TGCAAAGCTC TGTGCTTAAT TAAAGACATG
    ATTGCAGCAG TGGATTTAAA AGTCAGCGAG TATGAGAAAA ACCAAAAATG GCTTGAGATC
    CTAAATAAGA TCGAAAACAA AACCTACACA AAGCTCAAAA ATGGCCACGT GTTTAGGAAG
    CAAGCACTGA TAAGTAAAGA AAGGACTCTC TTATATGATG GCCTTGTTTA TTGGAAAACT
    GCTACGGGTC GTTTCAAAGA TATCCTGGCT CTACTTCTAA CTGATGTGCT GCTCTTTTTA
    CAAGAAAAAG ACCAGAAATA CATCTTTGCA GCTGTTGATC AGAAGCCATC GGTTATTTCC
    CTTCAGAAGC TCATTGCTAG AGAAGTCGCC AACGAGGAAA GAGGAATGTT CCTGATCAGT
    GCATCCTCTG CTGGTCCTGA GATGTACGAA ATTCACACCA ACTCCAAGGA GGAACGAAAT
    AACTGGATGA GACGAATCCA ACAGGCCGTG GAAAGTTGCC CAGAAGAAGA AGGGGGAAGG
    ACAAGTGAAT CTGATGAAGA GAGGCGGAAA GCTGAAGCAA GAGTGGCCAA AATCCAGCAA
    TGTCAAGAAA TACTCAGTAA CCAAGACCAA CAGATTTGCG CATATTTGGA GGAGAAGCTG
    CATATCTACG CTGAACTTGG AGAACTGAGT GGATTCAAGG ATGTCCATCT AGAGCCCCAC
    CTCCTCATCA AACCTGACCC AGGGGAGCCT CCCCAGGCAG CCTCATTACT AGCAGCAGCC
    CTGAGAGAAG CGGAGAGCCT GCAGGTTGCC GTGAAGGCCT CACAAGCGGG TGATGCTCGC
    CCATCACCTG AGGAGGGTTC TGCAGAACCT GCGGTTGCAG ATGCACAGGG TCCTCCCGAG
    GAGCCAGGAT CATCTCCAGC CTCACTAGTC ACAGAAGGGA CAGGAGGAAG AGGCTGTTGG
    GATGTGGATC CTGGCCCCCA GGGTGTGGGA ACCGACTTGG CGGTCTCTGA TGCAGGGGAG
    AAGAGTCTTA AAAATCTGTG CTGCAGCCGG CAAAGTCCCT GGGGTGTCAG TCACCATCAC
    CAGCTGCAGC CCTGCACAGG GACCACAAGG GGGAGGGCCA GCTCCACGAA AAGTAGGACA
    CTACACAGCT CAGGCTTGTA TCAGGCTTCG TGGTTCTTGG GAGTGGAATA TAGAAGTTTC
    ACGGTTTCTT CACAGTCAGA GATTATACAA GCGATCCAGA ATTTAACCCG ACTCCTCTAT
    AGCCTTCAGG CCGCGCTGAC CATCCAGGAC AGCCACATCC AGGTGCACAG GCTGCTGCTG
    CAGCAGGCTG GCCTGCCGGC GTCCCAAGAG GAGCACGGCG AGCTGGCGAG GCAGCGGGAG
    GAGCTGGCCG CCCTGCCCGG GCTCCAGTGG CAGCTGCAGC AGGAGCGGCG GCGCGGCCAG
    CGCACCTGGG AGCGGCAGCA GCGCGAGCAG GAGGCCAGGG CCTGCCGGCT GCGGGAGCGC
    GAGCAGGGCT GCCGCGGCCG CGAGGAGCTG CTGCTCAGGG CCCGCGGGGA GCTGGACCGG
    CAGCTGCGGG AGCACCGGCA GGACCTGGAG CGGCTGCGGG AGGGCCAGCG CCTGGTGGAG
    AGTGCGAGGG CCCGCACGCG CGCCCAGCAC AGCCTGCTGC GTGGCCCGAG GCCCGGGCGC
    CAGAGCAGCC TGCCCGCGGC CTTGCCTCCG GGCAGCGGCG AGGTGATGGA ACTTAATCGA
    TCCGAGAGCT TGTGTCATGA AAATTCATTC TTCATTAATG AAGCTTTAGT ACAAATGTCA
    CTTAACACTT ACAACAAATC AACTCCATCA GGTGTCCACC AGGAAGCCGC TTATCCCCAG
    AATATAGCTA ATTCGGACTT GGTAAGGACT AGTGAAAGTC CCGTAGACCT CCAGTTGGAC
    ATTTCTCAGC CCTTGGATGT CAACCACGAA CTGTGGACAG CCGCTGGGTC CTGTCATCAG
    ATACCCCCTC TCCACGAAAG CAGCAAGGAT TCTTGTAAAA ATGGAAGAAT TTACATCAGT
    CAGCTATGTT TCCAGAGCCA TTCGTCAGAA ATGTTTTGGC GACCAGACTT GACCATCTCA
    GCTTGGGATA GAGAGCTTCC GAATCTGCAG AACTTCCAAC CGCTGTTGGT TGCATTATGT
    AGATGGTTGC GTTACGTCCG TACATCCCCG GGAGATCTTG CCGTCATCCT CGAGTCCCCT
    GAGCAGGTTT TTCAGCTAGA AAGAAAATGT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35387
    Clone ID Related Accession (Same CDS sequence) XM_014109081.2 , XM_022410519.1 , XM_014109081.1
    Accession Version XM_022410519.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4173bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rho guanine nucleotide exchange factor 28 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGTGATTG ACCAGGTTGG TGATTTGGAT ATTAACTACA TCAATTTAGA AGGAATCTCT 
    ACAGACGCCT GTGCTGAATC CATGGGTTCT ACTCTGGGGC CTCAGAACTG CGAGCATATC
    CTTGCTGCTG AAACCAGCCA TGGGAAATAC CCACTAAGTG AGAACAAGGA AGTGGTCTCA
    GATACCGAAG CTCAGCAGTC CTTCCCATCA CCGTCTAGTT CATATGCTTC CAATTTTGAT
    CTTTCCTTTG GTCTTCATGG ATTTGAGAAG GAACAAAGTC TCCTGAAGAA AAGAAGCTCC
    AGCCTGGATG CCTTGGATGC CGACAGCGAA GGGGAAGGGC ATTCTGAGCG GTCACACATC
    TGCTACACGC CAGCATCTCA GAGTTCCTCG AGGACTGGGA TTCCCAGTGG GGATGAGCTG
    GACTCCTTCG AGACCGCCAC CAAACCTGAT TTTAGTATCT CCAGGACAGA GTCGCTTTCT
    TTATCAAGTA GCCTGCAGTT GAAGGAATCA TTGCTTCCTG GAGTCCGCTC ACGTTCTTAT
    TCCTGCTCAT CACCCAAAAT TTCTTTAGGA AAAACTCGAT TGGTGCGTGA TTTCACAGTG
    TGCAATTCTG GTGAAGAGCA AAGAGCCTTC GGCTTCCCGG AGCCCGCCAG GGAGAGGAGG
    ATTCAAGAGG AAGAATGGGA TAAATACATC ATACCTGCCA AGTCAGAGTC TGAGAAATAC
    AAAGTGAGCC GGACCTTCAG CTTCCTCATG AACAGGATGA CAAGCCCTCG GAACAAATCT
    AAGACGAAAA GCAAGGATGC CAAAGACAAA GAGAAACCGA GCCGACATCA GTTTGTCCCC
    GGAACCTTCT CTGGGGTTCT CCAGTGTTCC GTTTGTGATA AAACGCTCCT GGGGAAGGAG
    TCATCCCAGT GTTCCAACTG TAATGCAAAC GTCCACAAGG GTTGTAAGGA CGCTGCACCT
    CCGTGTACCA AGAAATTCCA GGAGAAATAT AACAAGAACA AACCGCAGAC CATCCTTGGA
    AACTCTTCAT TTCGAGACAT CCCGCAACCC AGTCTCTCCT TGCACCCTTC TTCCTCCATG
    CCTATCGGAT TGCCCACTGG GAGAAAAGAA GCTTCTGCCC AGGCCCATCC CTTGTCCAGA
    AGTGTTCCAG GCACTGCCTT GGAAAGCTTC AGAAGATCGG GGCTATCCCT GGAGTCTGAG
    AGTGACGGGA ACAGCTGGAG AAGCAGGTCT CATTCCGATG AGCTGTTACA GTCCATGGGC
    TCTTCTCCCT CCACGGAATC CTTCTTAATG GAAGATGTCG TGGACTCTTC TCTATGGAGT
    GACCTCAGCA GTGACGCCCA GGAGTTGGAA GCAGAATCCT GGAGTCTGGT GGTGGACCCC
    TCCTTTTGCA ATAAGCAGGA GAAAGATGTC ATCAAAAGAC AGGATGTCAT TTTCGAGCTT
    ATGCAAACAG AGATGCATCA TATCCAGACC CTGCTCATCA TGTCTGAGAT CTTCAGGAAA
    GGAATGAAGG AGGAGCTGCA GCTTGATCAC AGCACCGTGG ATAAAATCTT TCCCTGCTTA
    GATGAGTTAC TTGAAATCCA CAGGCATTTC TTTTATAGTA TGAAGGAGCG AAGGCAGGAG
    TCCTGTGCTG GTGGTGACAG GAATTTTGTC ATCAGCCGAA TTGGAGATAT TCTAGTACAG
    CAGTTTTCAG AAGAAAATGC AAATCAAATG AAGAAAATAT ATGGAGAATT CTGTAGCCAT
    CACAAAGAAG CTGTTAGCCT CTATAAAGAA CTCCAACAGA ATAAGAAGTT TCAGAATTTT
    ATTAAGCTTC GAAATAGTAA TCTTTTGGCT CGACGCCGAG GAATCCCAGA ATGCATTCTG
    CTAGTCACTC AGCGCATCAC AAAATACCCT GTCCTGGTGG AACGGATTTT GCAGTACACA
    AAGGAAAGAA GTGAAGAACA TCAAGACTTG TGCAAAGCTC TGTGCTTAAT TAAAGACATG
    ATTGCAGCAG TGGATTTAAA AGTCAGCGAG TATGAGAAAA ACCAAAAATG GCTTGAGATC
    CTAAATAAGA TCGAAAACAA AACCTACACA AAGCTCAAAA ATGGCCACGT GTTTAGGAAG
    CAAGCACTGA TAAGTAAAGA AAGGACTCTC TTATATGATG GCCTTGTTTA TTGGAAAACT
    GCTACGGGTC GTTTCAAAGA TATCCTGGCT CTACTTCTAA CTGATGTGCT GCTCTTTTTA
    CAAGAAAAAG ACCAGAAATA CATCTTTGCA GCTGTTGATC AGAAGCCATC GGTTATTTCC
    CTTCAGAAGC TCATTGCTAG AGAAGTCGCC AACGAGGAAA GAGGAATGTT CCTGATCAGT
    GCATCCTCTG CTGGTCCTGA GATGTACGAA ATTCACACCA ACTCCAAGGA GGAACGAAAT
    AACTGGATGA GACGAATCCA ACAGGCCGTG GAAAGTTGCC CAGAAGAAGA AGGGGGAAGG
    ACAAGTGAAT CTGATGAAGA GAGGCGGAAA GCTGAAGCAA GAGTGGCCAA AATCCAGCAA
    TGTCAAGAAA TACTCAGTAA CCAAGACCAA CAGATTTGCG CATATTTGGA GGAGAAGCTG
    CATATCTACG CTGAACTTGG AGAACTGAGT GGATTCAAGG ATGTCCATCT AGAGCCCCAC
    CTCCTCATCA AACCTGACCC AGGGGAGCCT CCCCAGGCAG CCTCATTACT AGCAGCAGCC
    CTGAGAGAAG CGGAGAGCCT GCAGGTTGCC GTGAAGGCCT CACAAGCGGG TGATGCTCGC
    CCATCACCTG AGGAGGGTTC TGCAGAACCT GCGGTTGCAG ATGCACAGGG TCCTCCCGAG
    GAGCCAGGAT CATCTCCAGC CTCACTAGTC ACAGAAGGGA CAGGAGGAAG AGGCTGTTGG
    GATGTGGATC CTGGCCCCCA GGGTGTGGGA ACCGACTTGG CGGTCTCTGA TGCAGGGGAG
    AAGAGTCTTA AAAATCTGTG CTGCAGCCGG CAAAGTCCCT GGGGTGTCAG TCACCATCAC
    CAGCTGCAGC CCTGCACAGG GACCACAAGG GGGAGGGCCA GCTCCACGAA AAGTAGGACA
    CTACACAGCT CAGGCTTGTA TCAGGCTTCG TGGTTCTTGG GAGTGGAATA TAGAAGTTTC
    ACGGTTTCTT CACAGTCAGA GATTATACAA GCGATCCAGA ATTTAACCCG ACTCCTCTAT
    AGCCTTCAGG CCGCGCTGAC CATCCAGGAC AGCCACATCC AGGTGCACAG GCTGCTGCTG
    CAGCAGGCTG GCCTGCCGGC GTCCCAAGAG GAGCACGGCG AGCTGGCGAG GCAGCGGGAG
    GAGCTGGCCG CCCTGCCCGG GCTCCAGTGG CAGCTGCAGC AGGAGCGGCG GCGCGGCCAG
    CGCACCTGGG AGCGGCAGCA GCGCGAGCAG GAGGCCAGGG CCTGCCGGCT GCGGGAGCGC
    GAGCAGGGCT GCCGCGGCCG CGAGGAGCTG CTGCTCAGGG CCCGCGGGGA GCTGGACCGG
    CAGCTGCGGG AGCACCGGCA GGACCTGGAG CGGCTGCGGG AGGGCCAGCG CCTGGTGGAG
    AGTGCGAGGG CCCGCACGCG CGCCCAGCAC AGCCTGCTGC GTGGCCCGAG GCCCGGGCGC
    CAGAGCAGCC TGCCCGCGGC CTTGCCTCCG GGCAGCGGCG AGGTGATGGA ACTTAATCGA
    TCCGAGAGCT TGTGTCATGA AAATTCATTC TTCATTAATG AAGCTTTAGT ACAAATGTCA
    CTTAACACTT ACAACAAATC AACTCCATCA GGTGTCCACC AGGAAGCCGC TTATCCCCAG
    AATATAGCTA ATTCGGACTT GGTAAGGACT AGTGAAAGTC CCGTAGACCT CCAGTTGGAC
    ATTTCTCAGC CCTTGGATGT CAACCACGAA CTGTGGACAG CCGCTGGGTC CTGTCATCAG
    ATACCCCCTC TCCACGAAAG CAGCAAGGAT TCTTGTAAAA ATGGAAGAAT TTACATCAGT
    CAGCTATGTT TCCAGAGCCA TTCGTCAGAA ATGTTTTGGC GACCAGACTT GACCATCTCA
    GCTTGGGATA GAGAGCTTCC GAATCTGCAG AACTTCCAAC CGCTGTTGGT TGCATTATGT
    AGATGGTTGC GTTACGTCCG TACATCCCCG GGAGATCTTG CCGTCATCCT CGAGTCCCCT
    GAGCAGGTTT TTCAGCTAGA AAGAAAATGT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022266227.1
    CDS240..4412
    Translation

    Target ORF information:

    RefSeq Version XM_022410519.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022410519.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGTGATTG ACCAGGTTGG TGATTTGGAT ATTAACTACA TCAATTTAGA AGGAATCTCT 
    ACAGACGCCT GTGCTGAATC CATGGGTTCT ACTCTGGGGC CTCAGAACTG CGAGCATATC
    CTTGCTGCTG AAACCAGCCA TGGGAAATAC CCACTAAGTG AGAACAAGGA AGTGGTCTCA
    GATACCGAAG CTCAGCAGTC CTTCCCATCA CCGTCTAGTT CATATGCTTC CAATTTTGAT
    CTTTCCTTTG GTCTTCATGG ATTTGAGAAG GAACAAAGTC TCCTGAAGAA AAGAAGCTCC
    AGCCTGGATG CCTTGGATGC CGACAGCGAA GGGGAAGGGC ATTCTGAGCG GTCACACATC
    TGCTACACGC CAGCATCTCA GAGTTCCTCG AGGACTGGGA TTCCCAGTGG GGATGAGCTG
    GACTCCTTCG AGACCGCCAC CAAACCTGAT TTTAGTATCT CCAGGACAGA GTCGCTTTCT
    TTATCAAGTA GCCTGCAGTT GAAGGAATCA TTGCTTCCTG GAGTCCGCTC ACGTTCTTAT
    TCCTGCTCAT CACCCAAAAT TTCTTTAGGA AAAACTCGAT TGGTGCGTGA TTTCACAGTG
    TGCAATTCTG GTGAAGAGCA AAGAGCCTTC GGCTTCCCGG AGCCCGCCAG GGAGAGGAGG
    ATTCAAGAGG AAGAATGGGA TAAATACATC ATACCTGCCA AGTCAGAGTC TGAGAAATAC
    AAAGTGAGCC GGACCTTCAG CTTCCTCATG AACAGGATGA CAAGCCCTCG GAACAAATCT
    AAGACGAAAA GCAAGGATGC CAAAGACAAA GAGAAACCGA GCCGACATCA GTTTGTCCCC
    GGAACCTTCT CTGGGGTTCT CCAGTGTTCC GTTTGTGATA AAACGCTCCT GGGGAAGGAG
    TCATCCCAGT GTTCCAACTG TAATGCAAAC GTCCACAAGG GTTGTAAGGA CGCTGCACCT
    CCGTGTACCA AGAAATTCCA GGAGAAATAT AACAAGAACA AACCGCAGAC CATCCTTGGA
    AACTCTTCAT TTCGAGACAT CCCGCAACCC AGTCTCTCCT TGCACCCTTC TTCCTCCATG
    CCTATCGGAT TGCCCACTGG GAGAAAAGAA GCTTCTGCCC AGGCCCATCC CTTGTCCAGA
    AGTGTTCCAG GCACTGCCTT GGAAAGCTTC AGAAGATCGG GGCTATCCCT GGAGTCTGAG
    AGTGACGGGA ACAGCTGGAG AAGCAGGTCT CATTCCGATG AGCTGTTACA GTCCATGGGC
    TCTTCTCCCT CCACGGAATC CTTCTTAATG GAAGATGTCG TGGACTCTTC TCTATGGAGT
    GACCTCAGCA GTGACGCCCA GGAGTTGGAA GCAGAATCCT GGAGTCTGGT GGTGGACCCC
    TCCTTTTGCA ATAAGCAGGA GAAAGATGTC ATCAAAAGAC AGGATGTCAT TTTCGAGCTT
    ATGCAAACAG AGATGCATCA TATCCAGACC CTGCTCATCA TGTCTGAGAT CTTCAGGAAA
    GGAATGAAGG AGGAGCTGCA GCTTGATCAC AGCACCGTGG ATAAAATCTT TCCCTGCTTA
    GATGAGTTAC TTGAAATCCA CAGGCATTTC TTTTATAGTA TGAAGGAGCG AAGGCAGGAG
    TCCTGTGCTG GTGGTGACAG GAATTTTGTC ATCAGCCGAA TTGGAGATAT TCTAGTACAG
    CAGTTTTCAG AAGAAAATGC AAATCAAATG AAGAAAATAT ATGGAGAATT CTGTAGCCAT
    CACAAAGAAG CTGTTAGCCT CTATAAAGAA CTCCAACAGA ATAAGAAGTT TCAGAATTTT
    ATTAAGCTTC GAAATAGTAA TCTTTTGGCT CGACGCCGAG GAATCCCAGA ATGCATTCTG
    CTAGTCACTC AGCGCATCAC AAAATACCCT GTCCTGGTGG AACGGATTTT GCAGTACACA
    AAGGAAAGAA GTGAAGAACA TCAAGACTTG TGCAAAGCTC TGTGCTTAAT TAAAGACATG
    ATTGCAGCAG TGGATTTAAA AGTCAGCGAG TATGAGAAAA ACCAAAAATG GCTTGAGATC
    CTAAATAAGA TCGAAAACAA AACCTACACA AAGCTCAAAA ATGGCCACGT GTTTAGGAAG
    CAAGCACTGA TAAGTAAAGA AAGGACTCTC TTATATGATG GCCTTGTTTA TTGGAAAACT
    GCTACGGGTC GTTTCAAAGA TATCCTGGCT CTACTTCTAA CTGATGTGCT GCTCTTTTTA
    CAAGAAAAAG ACCAGAAATA CATCTTTGCA GCTGTTGATC AGAAGCCATC GGTTATTTCC
    CTTCAGAAGC TCATTGCTAG AGAAGTCGCC AACGAGGAAA GAGGAATGTT CCTGATCAGT
    GCATCCTCTG CTGGTCCTGA GATGTACGAA ATTCACACCA ACTCCAAGGA GGAACGAAAT
    AACTGGATGA GACGAATCCA ACAGGCCGTG GAAAGTTGCC CAGAAGAAGA AGGGGGAAGG
    ACAAGTGAAT CTGATGAAGA GAGGCGGAAA GCTGAAGCAA GAGTGGCCAA AATCCAGCAA
    TGTCAAGAAA TACTCAGTAA CCAAGACCAA CAGATTTGCG CATATTTGGA GGAGAAGCTG
    CATATCTACG CTGAACTTGG AGAACTGAGT GGATTCAAGG ATGTCCATCT AGAGCCCCAC
    CTCCTCATCA AACCTGACCC AGGGGAGCCT CCCCAGGCAG CCTCATTACT AGCAGCAGCC
    CTGAGAGAAG CGGAGAGCCT GCAGGTTGCC GTGAAGGCCT CACAAGCGGG TGATGCTCGC
    CCATCACCTG AGGAGGGTTC TGCAGAACCT GCGGTTGCAG ATGCACAGGG TCCTCCCGAG
    GAGCCAGGAT CATCTCCAGC CTCACTAGTC ACAGAAGGGA CAGGAGGAAG AGGCTGTTGG
    GATGTGGATC CTGGCCCCCA GGGTGTGGGA ACCGACTTGG CGGTCTCTGA TGCAGGGGAG
    AAGAGTCTTA AAAATCTGTG CTGCAGCCGG CAAAGTCCCT GGGGTGTCAG TCACCATCAC
    CAGCTGCAGC CCTGCACAGG GACCACAAGG GGGAGGGCCA GCTCCACGAA AAGTAGGACA
    CTACACAGCT CAGGCTTGTA TCAGGCTTCG TGGTTCTTGG GAGTGGAATA TAGAAGTTTC
    ACGGTTTCTT CACAGTCAGA GATTATACAA GCGATCCAGA ATTTAACCCG ACTCCTCTAT
    AGCCTTCAGG CCGCGCTGAC CATCCAGGAC AGCCACATCC AGGTGCACAG GCTGCTGCTG
    CAGCAGGCTG GCCTGCCGGC GTCCCAAGAG GAGCACGGCG AGCTGGCGAG GCAGCGGGAG
    GAGCTGGCCG CCCTGCCCGG GCTCCAGTGG CAGCTGCAGC AGGAGCGGCG GCGCGGCCAG
    CGCACCTGGG AGCGGCAGCA GCGCGAGCAG GAGGCCAGGG CCTGCCGGCT GCGGGAGCGC
    GAGCAGGGCT GCCGCGGCCG CGAGGAGCTG CTGCTCAGGG CCCGCGGGGA GCTGGACCGG
    CAGCTGCGGG AGCACCGGCA GGACCTGGAG CGGCTGCGGG AGGGCCAGCG CCTGGTGGAG
    AGTGCGAGGG CCCGCACGCG CGCCCAGCAC AGCCTGCTGC GTGGCCCGAG GCCCGGGCGC
    CAGAGCAGCC TGCCCGCGGC CTTGCCTCCG GGCAGCGGCG AGGTGATGGA ACTTAATCGA
    TCCGAGAGCT TGTGTCATGA AAATTCATTC TTCATTAATG AAGCTTTAGT ACAAATGTCA
    CTTAACACTT ACAACAAATC AACTCCATCA GGTGTCCACC AGGAAGCCGC TTATCCCCAG
    AATATAGCTA ATTCGGACTT GGTAAGGACT AGTGAAAGTC CCGTAGACCT CCAGTTGGAC
    ATTTCTCAGC CCTTGGATGT CAACCACGAA CTGTGGACAG CCGCTGGGTC CTGTCATCAG
    ATACCCCCTC TCCACGAAAG CAGCAAGGAT TCTTGTAAAA ATGGAAGAAT TTACATCAGT
    CAGCTATGTT TCCAGAGCCA TTCGTCAGAA ATGTTTTGGC GACCAGACTT GACCATCTCA
    GCTTGGGATA GAGAGCTTCC GAATCTGCAG AACTTCCAAC CGCTGTTGGT TGCATTATGT
    AGATGGTTGC GTTACGTCCG TACATCCCCG GGAGATCTTG CCGTCATCCT CGAGTCCCCT
    GAGCAGGTTT TTCAGCTAGA AAGAAAATGT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35387
    Clone ID Related Accession (Same CDS sequence) XM_014109081.2 , XM_022410519.1 , XM_014109081.1
    Accession Version XM_014109081.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4173bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product rho guanine nucleotide exchange factor 28 isoform X11
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGTGATTG ACCAGGTTGG TGATTTGGAT ATTAACTACA TCAATTTAGA AGGAATCTCT 
    ACAGACGCCT GTGCTGAATC CATGGGTTCT ACTCTGGGGC CTCAGAACTG CGAGCATATC
    CTTGCTGCTG AAACCAGCCA TGGGAAATAC CCACTAAGTG AGAACAAGGA AGTGGTCTCA
    GATACCGAAG CTCAGCAGTC CTTCCCATCA CCGTCTAGTT CATATGCTTC CAATTTTGAT
    CTTTCCTTTG GTCTTCATGG ATTTGAGAAG GAACAAAGTC TCCTGAAGAA AAGAAGCTCC
    AGCCTGGATG CCTTGGATGC CGACAGCGAA GGGGAAGGGC ATTCTGAGCG GTCACACATC
    TGCTACACGC CAGCATCTCA GAGTTCCTCG AGGACTGGGA TTCCCAGTGG GGATGAGCTG
    GACTCCTTCG AGACCGCCAC CAAACCTGAT TTTAGTATCT CCAGGACAGA GTCGCTTTCT
    TTATCAAGTA GCCTGCAGTT GAAGGAATCA TTGCTTCCTG GAGTCCGCTC ACGTTCTTAT
    TCCTGCTCAT CACCCAAAAT TTCTTTAGGA AAAACTCGAT TGGTGCGTGA TTTCACAGTG
    TGCAATTCTG GTGAAGAGCA AAGAGCCTTC GGCTTCCCGG AGCCCGCCAG GGAGAGGAGG
    ATTCAAGAGG AAGAATGGGA TAAATACATC ATACCTGCCA AGTCAGAGTC TGAGAAATAC
    AAAGTGAGCC GGACCTTCAG CTTCCTCATG AACAGGATGA CAAGCCCTCG GAACAAATCT
    AAGACGAAAA GCAAGGATGC CAAAGACAAA GAGAAACCGA GCCGACATCA GTTTGTCCCC
    GGAACCTTCT CTGGGGTTCT CCAGTGTTCC GTTTGTGATA AAACGCTCCT GGGGAAGGAG
    TCATCCCAGT GTTCCAACTG TAATGCAAAC GTCCACAAGG GTTGTAAGGA CGCTGCACCT
    CCGTGTACCA AGAAATTCCA GGAGAAATAT AACAAGAACA AACCGCAGAC CATCCTTGGA
    AACTCTTCAT TTCGAGACAT CCCGCAACCC AGTCTCTCCT TGCACCCTTC TTCCTCCATG
    CCTATCGGAT TGCCCACTGG GAGAAAAGAA GCTTCTGCCC AGGCCCATCC CTTGTCCAGA
    AGTGTTCCAG GCACTGCCTT GGAAAGCTTC AGAAGATCGG GGCTATCCCT GGAGTCTGAG
    AGTGACGGGA ACAGCTGGAG AAGCAGGTCT CATTCCGATG AGCTGTTACA GTCCATGGGC
    TCTTCTCCCT CCACGGAATC CTTCTTAATG GAAGATGTCG TGGACTCTTC TCTATGGAGT
    GACCTCAGCA GTGACGCCCA GGAGTTGGAA GCAGAATCCT GGAGTCTGGT GGTGGACCCC
    TCCTTTTGCA ATAAGCAGGA GAAAGATGTC ATCAAAAGAC AGGATGTCAT TTTCGAGCTT
    ATGCAAACAG AGATGCATCA TATCCAGACC CTGCTCATCA TGTCTGAGAT CTTCAGGAAA
    GGAATGAAGG AGGAGCTGCA GCTTGATCAC AGCACCGTGG ATAAAATCTT TCCCTGCTTA
    GATGAGTTAC TTGAAATCCA CAGGCATTTC TTTTATAGTA TGAAGGAGCG AAGGCAGGAG
    TCCTGTGCTG GTGGTGACAG GAATTTTGTC ATCAGCCGAA TTGGAGATAT TCTAGTACAG
    CAGTTTTCAG AAGAAAATGC AAATCAAATG AAGAAAATAT ATGGAGAATT CTGTAGCCAT
    CACAAAGAAG CTGTTAGCCT CTATAAAGAA CTCCAACAGA ATAAGAAGTT TCAGAATTTT
    ATTAAGCTTC GAAATAGTAA TCTTTTGGCT CGACGCCGAG GAATCCCAGA ATGCATTCTG
    CTAGTCACTC AGCGCATCAC AAAATACCCT GTCCTGGTGG AACGGATTTT GCAGTACACA
    AAGGAAAGAA GTGAAGAACA TCAAGACTTG TGCAAAGCTC TGTGCTTAAT TAAAGACATG
    ATTGCAGCAG TGGATTTAAA AGTCAGCGAG TATGAGAAAA ACCAAAAATG GCTTGAGATC
    CTAAATAAGA TCGAAAACAA AACCTACACA AAGCTCAAAA ATGGCCACGT GTTTAGGAAG
    CAAGCACTGA TAAGTAAAGA AAGGACTCTC TTATATGATG GCCTTGTTTA TTGGAAAACT
    GCTACGGGTC GTTTCAAAGA TATCCTGGCT CTACTTCTAA CTGATGTGCT GCTCTTTTTA
    CAAGAAAAAG ACCAGAAATA CATCTTTGCA GCTGTTGATC AGAAGCCATC GGTTATTTCC
    CTTCAGAAGC TCATTGCTAG AGAAGTCGCC AACGAGGAAA GAGGAATGTT CCTGATCAGT
    GCATCCTCTG CTGGTCCTGA GATGTACGAA ATTCACACCA ACTCCAAGGA GGAACGAAAT
    AACTGGATGA GACGAATCCA ACAGGCCGTG GAAAGTTGCC CAGAAGAAGA AGGGGGAAGG
    ACAAGTGAAT CTGATGAAGA GAGGCGGAAA GCTGAAGCAA GAGTGGCCAA AATCCAGCAA
    TGTCAAGAAA TACTCAGTAA CCAAGACCAA CAGATTTGCG CATATTTGGA GGAGAAGCTG
    CATATCTACG CTGAACTTGG AGAACTGAGT GGATTCAAGG ATGTCCATCT AGAGCCCCAC
    CTCCTCATCA AACCTGACCC AGGGGAGCCT CCCCAGGCAG CCTCATTACT AGCAGCAGCC
    CTGAGAGAAG CGGAGAGCCT GCAGGTTGCC GTGAAGGCCT CACAAGCGGG TGATGCTCGC
    CCATCACCTG AGGAGGGTTC TGCAGAACCT GCGGTTGCAG ATGCACAGGG TCCTCCCGAG
    GAGCCAGGAT CATCTCCAGC CTCACTAGTC ACAGAAGGGA CAGGAGGAAG AGGCTGTTGG
    GATGTGGATC CTGGCCCCCA GGGTGTGGGA ACCGACTTGG CGGTCTCTGA TGCAGGGGAG
    AAGAGTCTTA AAAATCTGTG CTGCAGCCGG CAAAGTCCCT GGGGTGTCAG TCACCATCAC
    CAGCTGCAGC CCTGCACAGG GACCACAAGG GGGAGGGCCA GCTCCACGAA AAGTAGGACA
    CTACACAGCT CAGGCTTGTA TCAGGCTTCG TGGTTCTTGG GAGTGGAATA TAGAAGTTTC
    ACGGTTTCTT CACAGTCAGA GATTATACAA GCGATCCAGA ATTTAACCCG ACTCCTCTAT
    AGCCTTCAGG CCGCGCTGAC CATCCAGGAC AGCCACATCC AGGTGCACAG GCTGCTGCTG
    CAGCAGGCTG GCCTGCCGGC GTCCCAAGAG GAGCACGGCG AGCTGGCGAG GCAGCGGGAG
    GAGCTGGCCG CCCTGCCCGG GCTCCAGTGG CAGCTGCAGC AGGAGCGGCG GCGCGGCCAG
    CGCACCTGGG AGCGGCAGCA GCGCGAGCAG GAGGCCAGGG CCTGCCGGCT GCGGGAGCGC
    GAGCAGGGCT GCCGCGGCCG CGAGGAGCTG CTGCTCAGGG CCCGCGGGGA GCTGGACCGG
    CAGCTGCGGG AGCACCGGCA GGACCTGGAG CGGCTGCGGG AGGGCCAGCG CCTGGTGGAG
    AGTGCGAGGG CCCGCACGCG CGCCCAGCAC AGCCTGCTGC GTGGCCCGAG GCCCGGGCGC
    CAGAGCAGCC TGCCCGCGGC CTTGCCTCCG GGCAGCGGCG AGGTGATGGA ACTTAATCGA
    TCCGAGAGCT TGTGTCATGA AAATTCATTC TTCATTAATG AAGCTTTAGT ACAAATGTCA
    CTTAACACTT ACAACAAATC AACTCCATCA GGTGTCCACC AGGAAGCCGC TTATCCCCAG
    AATATAGCTA ATTCGGACTT GGTAAGGACT AGTGAAAGTC CCGTAGACCT CCAGTTGGAC
    ATTTCTCAGC CCTTGGATGT CAACCACGAA CTGTGGACAG CCGCTGGGTC CTGTCATCAG
    ATACCCCCTC TCCACGAAAG CAGCAAGGAT TCTTGTAAAA ATGGAAGAAT TTACATCAGT
    CAGCTATGTT TCCAGAGCCA TTCGTCAGAA ATGTTTTGGC GACCAGACTT GACCATCTCA
    GCTTGGGATA GAGAGCTTCC GAATCTGCAG AACTTCCAAC CGCTGTTGGT TGCATTATGT
    AGATGGTTGC GTTACGTCCG TACATCCCCG GGAGATCTTG CCGTCATCCT CGAGTCCCCT
    GAGCAGGTTT TTCAGCTAGA AAGAAAATGT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013964556.1
    CDS611..4783
    Translation

    Target ORF information:

    RefSeq Version XM_014109081.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 28 (ARHGEF28), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109081.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGTGATTG ACCAGGTTGG TGATTTGGAT ATTAACTACA TCAATTTAGA AGGAATCTCT 
    ACAGACGCCT GTGCTGAATC CATGGGTTCT ACTCTGGGGC CTCAGAACTG CGAGCATATC
    CTTGCTGCTG AAACCAGCCA TGGGAAATAC CCACTAAGTG AGAACAAGGA AGTGGTCTCA
    GATACCGAAG CTCAGCAGTC CTTCCCATCA CCGTCTAGTT CATATGCTTC CAATTTTGAT
    CTTTCCTTTG GTCTTCATGG ATTTGAGAAG GAACAAAGTC TCCTGAAGAA AAGAAGCTCC
    AGCCTGGATG CCTTGGATGC CGACAGCGAA GGGGAAGGGC ATTCTGAGCG GTCACACATC
    TGCTACACGC CAGCATCTCA GAGTTCCTCG AGGACTGGGA TTCCCAGTGG GGATGAGCTG
    GACTCCTTCG AGACCGCCAC CAAACCTGAT TTTAGTATCT CCAGGACAGA GTCGCTTTCT
    TTATCAAGTA GCCTGCAGTT GAAGGAATCA TTGCTTCCTG GAGTCCGCTC ACGTTCTTAT
    TCCTGCTCAT CACCCAAAAT TTCTTTAGGA AAAACTCGAT TGGTGCGTGA TTTCACAGTG
    TGCAATTCTG GTGAAGAGCA AAGAGCCTTC GGCTTCCCGG AGCCCGCCAG GGAGAGGAGG
    ATTCAAGAGG AAGAATGGGA TAAATACATC ATACCTGCCA AGTCAGAGTC TGAGAAATAC
    AAAGTGAGCC GGACCTTCAG CTTCCTCATG AACAGGATGA CAAGCCCTCG GAACAAATCT
    AAGACGAAAA GCAAGGATGC CAAAGACAAA GAGAAACCGA GCCGACATCA GTTTGTCCCC
    GGAACCTTCT CTGGGGTTCT CCAGTGTTCC GTTTGTGATA AAACGCTCCT GGGGAAGGAG
    TCATCCCAGT GTTCCAACTG TAATGCAAAC GTCCACAAGG GTTGTAAGGA CGCTGCACCT
    CCGTGTACCA AGAAATTCCA GGAGAAATAT AACAAGAACA AACCGCAGAC CATCCTTGGA
    AACTCTTCAT TTCGAGACAT CCCGCAACCC AGTCTCTCCT TGCACCCTTC TTCCTCCATG
    CCTATCGGAT TGCCCACTGG GAGAAAAGAA GCTTCTGCCC AGGCCCATCC CTTGTCCAGA
    AGTGTTCCAG GCACTGCCTT GGAAAGCTTC AGAAGATCGG GGCTATCCCT GGAGTCTGAG
    AGTGACGGGA ACAGCTGGAG AAGCAGGTCT CATTCCGATG AGCTGTTACA GTCCATGGGC
    TCTTCTCCCT CCACGGAATC CTTCTTAATG GAAGATGTCG TGGACTCTTC TCTATGGAGT
    GACCTCAGCA GTGACGCCCA GGAGTTGGAA GCAGAATCCT GGAGTCTGGT GGTGGACCCC
    TCCTTTTGCA ATAAGCAGGA GAAAGATGTC ATCAAAAGAC AGGATGTCAT TTTCGAGCTT
    ATGCAAACAG AGATGCATCA TATCCAGACC CTGCTCATCA TGTCTGAGAT CTTCAGGAAA
    GGAATGAAGG AGGAGCTGCA GCTTGATCAC AGCACCGTGG ATAAAATCTT TCCCTGCTTA
    GATGAGTTAC TTGAAATCCA CAGGCATTTC TTTTATAGTA TGAAGGAGCG AAGGCAGGAG
    TCCTGTGCTG GTGGTGACAG GAATTTTGTC ATCAGCCGAA TTGGAGATAT TCTAGTACAG
    CAGTTTTCAG AAGAAAATGC AAATCAAATG AAGAAAATAT ATGGAGAATT CTGTAGCCAT
    CACAAAGAAG CTGTTAGCCT CTATAAAGAA CTCCAACAGA ATAAGAAGTT TCAGAATTTT
    ATTAAGCTTC GAAATAGTAA TCTTTTGGCT CGACGCCGAG GAATCCCAGA ATGCATTCTG
    CTAGTCACTC AGCGCATCAC AAAATACCCT GTCCTGGTGG AACGGATTTT GCAGTACACA
    AAGGAAAGAA GTGAAGAACA TCAAGACTTG TGCAAAGCTC TGTGCTTAAT TAAAGACATG
    ATTGCAGCAG TGGATTTAAA AGTCAGCGAG TATGAGAAAA ACCAAAAATG GCTTGAGATC
    CTAAATAAGA TCGAAAACAA AACCTACACA AAGCTCAAAA ATGGCCACGT GTTTAGGAAG
    CAAGCACTGA TAAGTAAAGA AAGGACTCTC TTATATGATG GCCTTGTTTA TTGGAAAACT
    GCTACGGGTC GTTTCAAAGA TATCCTGGCT CTACTTCTAA CTGATGTGCT GCTCTTTTTA
    CAAGAAAAAG ACCAGAAATA CATCTTTGCA GCTGTTGATC AGAAGCCATC GGTTATTTCC
    CTTCAGAAGC TCATTGCTAG AGAAGTCGCC AACGAGGAAA GAGGAATGTT CCTGATCAGT
    GCATCCTCTG CTGGTCCTGA GATGTACGAA ATTCACACCA ACTCCAAGGA GGAACGAAAT
    AACTGGATGA GACGAATCCA ACAGGCCGTG GAAAGTTGCC CAGAAGAAGA AGGGGGAAGG
    ACAAGTGAAT CTGATGAAGA GAGGCGGAAA GCTGAAGCAA GAGTGGCCAA AATCCAGCAA
    TGTCAAGAAA TACTCAGTAA CCAAGACCAA CAGATTTGCG CATATTTGGA GGAGAAGCTG
    CATATCTACG CTGAACTTGG AGAACTGAGT GGATTCAAGG ATGTCCATCT AGAGCCCCAC
    CTCCTCATCA AACCTGACCC AGGGGAGCCT CCCCAGGCAG CCTCATTACT AGCAGCAGCC
    CTGAGAGAAG CGGAGAGCCT GCAGGTTGCC GTGAAGGCCT CACAAGCGGG TGATGCTCGC
    CCATCACCTG AGGAGGGTTC TGCAGAACCT GCGGTTGCAG ATGCACAGGG TCCTCCCGAG
    GAGCCAGGAT CATCTCCAGC CTCACTAGTC ACAGAAGGGA CAGGAGGAAG AGGCTGTTGG
    GATGTGGATC CTGGCCCCCA GGGTGTGGGA ACCGACTTGG CGGTCTCTGA TGCAGGGGAG
    AAGAGTCTTA AAAATCTGTG CTGCAGCCGG CAAAGTCCCT GGGGTGTCAG TCACCATCAC
    CAGCTGCAGC CCTGCACAGG GACCACAAGG GGGAGGGCCA GCTCCACGAA AAGTAGGACA
    CTACACAGCT CAGGCTTGTA TCAGGCTTCG TGGTTCTTGG GAGTGGAATA TAGAAGTTTC
    ACGGTTTCTT CACAGTCAGA GATTATACAA GCGATCCAGA ATTTAACCCG ACTCCTCTAT
    AGCCTTCAGG CCGCGCTGAC CATCCAGGAC AGCCACATCC AGGTGCACAG GCTGCTGCTG
    CAGCAGGCTG GCCTGCCGGC GTCCCAAGAG GAGCACGGCG AGCTGGCGAG GCAGCGGGAG
    GAGCTGGCCG CCCTGCCCGG GCTCCAGTGG CAGCTGCAGC AGGAGCGGCG GCGCGGCCAG
    CGCACCTGGG AGCGGCAGCA GCGCGAGCAG GAGGCCAGGG CCTGCCGGCT GCGGGAGCGC
    GAGCAGGGCT GCCGCGGCCG CGAGGAGCTG CTGCTCAGGG CCCGCGGGGA GCTGGACCGG
    CAGCTGCGGG AGCACCGGCA GGACCTGGAG CGGCTGCGGG AGGGCCAGCG CCTGGTGGAG
    AGTGCGAGGG CCCGCACGCG CGCCCAGCAC AGCCTGCTGC GTGGCCCGAG GCCCGGGCGC
    CAGAGCAGCC TGCCCGCGGC CTTGCCTCCG GGCAGCGGCG AGGTGATGGA ACTTAATCGA
    TCCGAGAGCT TGTGTCATGA AAATTCATTC TTCATTAATG AAGCTTTAGT ACAAATGTCA
    CTTAACACTT ACAACAAATC AACTCCATCA GGTGTCCACC AGGAAGCCGC TTATCCCCAG
    AATATAGCTA ATTCGGACTT GGTAAGGACT AGTGAAAGTC CCGTAGACCT CCAGTTGGAC
    ATTTCTCAGC CCTTGGATGT CAACCACGAA CTGTGGACAG CCGCTGGGTC CTGTCATCAG
    ATACCCCCTC TCCACGAAAG CAGCAAGGAT TCTTGTAAAA ATGGAAGAAT TTACATCAGT
    CAGCTATGTT TCCAGAGCCA TTCGTCAGAA ATGTTTTGGC GACCAGACTT GACCATCTCA
    GCTTGGGATA GAGAGCTTCC GAATCTGCAG AACTTCCAAC CGCTGTTGGT TGCATTATGT
    AGATGGTTGC GTTACGTCCG TACATCCCCG GGAGATCTTG CCGTCATCCT CGAGTCCCCT
    GAGCAGGTTT TTCAGCTAGA AAGAAAATGT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35388
    Clone ID Related Accession (Same CDS sequence) XM_014109082.1 , XM_014109082.2
    Accession Version XM_014109082.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4365bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product rho guanine nucleotide exchange factor 28 isoform X12
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGACTCGG ACTCGGACTC CCCTTTGAAC TACTCGTGGC CGTCCTTCCC CAAGATGAAG 
    ACGCGCCGGA GGGCGGCCAA GCCAGATCAC TCTTTCGATG TCTTCAAAAA ACCTAAGCAT
    CCCCCAGCGT TCTTTGCTGC AGGCGGGCTC TCTGATGTGC TGAATGGCGG CGATGAAGTT
    TACGCCAACT GTATGGTGAT TGACCAGGTT GGTGATTTGG ATATTAACTA CATCAATTTA
    GAAGGAATCT CTACAGACGC CTGTGCTGAA TCCATGGGTT CTACTCTGGG GCCTCAGAAC
    TGCGAGCATA TCCTTGCTGC TGAAACCAGC CATGGGAAAT ACCCACTAAG TGAGAACAAG
    GAAGTGGTCT CAGATACCGA AGCTCAGCAG TCCTTCCCAT CACCGTCTAG TTCATATGCT
    TCCAATTTTG ATCTTTCCTT TGGTCTTCAT GGATTTGAGA AGGAACAAAG TCTCCTGAAG
    AAAAGAAGCT CCAGCCTGGA TGCCTTGGAT GCCGACAGCG AAGGGGAAGG GCATTCTGAG
    CGGTCACACA TCTGCTACAC GCCAGCATCT CAGAGTTCCT CGAGGACTGG GATTCCCAGT
    GGGGATGAGC TGGACTCCTT CGAGACCGCC ACCAAACCTG ATTTTAGTAT CTCCAGGACA
    GAGTCGCTTT CTTTATCAAG TAGCCTGCAG TTGAAGGAAT CATTGCTTCC TGGAGTCCGC
    TCACGTTCTT ATTCCTGCTC ATCACCCAAA ATTTCTTTAG GAAAAACTCG ATTGGTGCGT
    GATTTCACAG TGTGCAATTC TGGTGAAGAG CAAAGAGCCT TCGGCTTCCC GGAGCCCGCC
    AGGGAGAGGA GGATTCAAGA GGAAGAATGG GATAAATACA TCATACCTGC CAAGTCAGAG
    TCTGAGAAAT ACAAAGTGAG CCGGACCTTC AGCTTCCTCA TGAACAGGAT GACAAGCCCT
    CGGAACAAAT CTAAGACGAA AAGCAAGGAT GCCAAAGACA AAGAGAAACC GAGCCGACAT
    CAGTTTGTCC CCGGAACCTT CTCTGGGGTT CTCCAGTGTT CCGTTTGTGA TAAAACGCTC
    CTGGGGAAGG AGTCATCCCA GTGTTCCAAC TGTAATGCAA ACGTCCACAA GGGTTGTAAG
    GACGCTGCAC CTCCGTGTAC CAAGAAATTC CAGGAGAAAT ATAACAAGAA CAAACCGCAG
    ACCATCCTTG GAAACTCTTC ATTTCGAGAC ATCCCGCAAC CCAGTCTCTC CTTGCACCCT
    TCTTCCTCCA TGCCTATCGG ATTGCCCACT GGGAGAAAAG AAGCTTCTGC CCAGGCCCAT
    CCCTTGTCCA GAAGTGTTCC AGGCACTGCC TTGGAAAGCT TCAGAAGATC GGGGCTATCC
    CTGGAGTCTG AGAGTGACGG GAACAGCTGG AGAAGCAGGT CTCATTCCGA TGAGCTGTTA
    CAGTCCATGG GCTCTTCTCC CTCCACGGAA TCCTTCTTAA TGGAAGATGT CGTGGACTCT
    TCTCTATGGA GTGACCTCAG CAGTGACGCC CAGGAGTTGG AAGCAGAATC CTGGAGTCTG
    GTGGTGGACC CCTCCTTTTG CAATAAGCAG GAGAAAGATG TCATCAAAAG ACAGGATGTC
    ATTTTCGAGC TTATGCAAAC AGAGATGCAT CATATCCAGA CCCTGCTCAT CATGTCTGAG
    ATCTTCAGGA AAGGAATGAA GGAGGAGCTG CAGCTTGATC ACAGCACCGT GGATAAAATC
    TTTCCCTGCT TAGATGAGTT ACTTGAAATC CACAGGCATT TCTTTTATAG TATGAAGGAG
    CGAAGGCAGG AGTCCTGTGC TGGTGGTGAC AGGAATTTTG TCATCAGCCG AATTGGAGAT
    ATTCTAGTAC AGCAGTTTTC AGAAGAAAAT GCAAATCAAA TGAAGAAAAT ATATGGAGAA
    TTCTGTAGCC ATCACAAAGA AGCTGTTAGC CTCTATAAAG AACTCCAACA GAATAAGAAG
    TTTCAGAATT TTATTAAGCT TCGAAATAGT AATCTTTTGG CTCGACGCCG AGGAATCCCA
    GAATGCATTC TGCTAGTCAC TCAGCGCATC ACAAAATACC CTGTCCTGGT GGAACGGATT
    TTGCAGTACA CAAAGGAAAG AAGTGAAGAA CATCAAGACT TGTGCAAAGC TCTGTGCTTA
    ATTAAAGACA TGATTGCAGC AGTGGATTTA AAAGTCAGCG AGTATGAGAA AAACCAAAAA
    TGGCTTGAGA TCCTAAATAA GATCGAAAAC AAAACCTACA CAAAGCTCAA AAATGGCCAC
    GTGTTTAGGA AGCAAGCACT GATAAGTAAA GAAAGGACTC TCTTATATGA TGGCCTTGTT
    TATTGGAAAA CTGCTACGGG TCGTTTCAAA GATATCCTGG CTCTACTTCT AACTGATGTG
    CTGCTCTTTT TACAAGAAAA AGACCAGAAA TACATCTTTG CAGCTGTTGA TCAGAAGCCA
    TCGGTTATTT CCCTTCAGAA GCTCATTGCT AGAGAAGTCG CCAACGAGGA AAGAGGAATG
    TTCCTGATCA GTGCATCCTC TGCTGGTCCT GAGATGTACG AAATTCACAC CAACTCCAAG
    GAGGAACGAA ATAACTGGAT GAGACGAATC CAACAGGCCG TGGAAAGTTG CCCAGAAGAA
    GAAGGGGGAA GGACAAGTGA ATCTGATGAA GAGAGGCGGA AAGCTGAAGC AAGAGTGGCC
    AAAATCCAGC AATGTCAAGA AATACTCAGT AACCAAGACC AACAGATTTG CGCATATTTG
    GAGGAGAAGC TGCATATCTA CGCTGAACTT GGAGAACTGA GTGGATTCAA GGATGTCCAT
    CTAGAGCCCC ACCTCCTCAT CAAACCTGAC CCAGGGGAGC CTCCCCAGGC AGCCTCATTA
    CTAGCAGCAG CCCTGAGAGA AGCGGAGAGC CTGCAGGTTG CCGTGAAGGC CTCACAAGCG
    GGTGATGCTC GCCCATCACC TGAGGAGGGT TCTGCAGAAC CTGCGGTTGC AGATGCACAG
    GGTCCTCCCG AGGAGCCAGG ATCATCTCCA GCCTCACTAG TCACAGAAGG GACAGGAGGA
    AGAGGCTGTT GGGATGTGGA TCCTGGCCCC CAGGGTGTGG GAACCGACTT GGCGGTCTCT
    GATGCAGGGG AGAAGAGTCT TAAAAATCTG TGCTGCAGCC GGCAAAGTCC CTGGGGTGTC
    AGTCACCATC ACCAGCTGCA GCCCTGCACA GGGACCACAA GGGGGAGGGC CAGCTCCACG
    AAAAGTAGGA CACTACACAG CTCAGGCTTG TATCAGGCTT CGTGGTTCTT GGGAGTGGAA
    TATAGAAGTT TCACGGTTTC TTCACAGTCA GAGATTATAC AAGCGATCCA GAATTTAACC
    CGACTCCTCT ATAGCCTTCA GGCCGCGCTG ACCATCCAGG ACAGCCACAT CCAGGTGCAC
    AGGCTGCTGC TGCAGCAGGC TGGCCTGCCG GCGTCCCAAG AGGAGCACGG CGAGCTGGCG
    AGGCAGCGGG AGGAGCTGGC CGCCCTGCCC GGGCTCCAGT GGCAGCTGCA GCAGGAGCGG
    CGGCGCGGCC AGCGCACCTG GGAGCGGCAG CAGCGCGAGC AGGAGGCCAG GGCCTGCCGG
    CTGCGGGAGC GCGAGCAGGG CTGCCGCGGC CGCGAGGAGC TGCTGCTCAG GGCCCGCGGG
    GAGCTGGACC GGCAGCTGCG GGAGCACCGG CAGGACCTGG AGCGGCTGCG GGAGGGCCAG
    CGCCTGGTGG AGAGTGCGAG GGCCCGCACG CGCGCCCAGC ACAGCCTGCT GCGTGGCCCG
    AGGCCCGGGC GCCAGAGCAG CCTGCCCGCG GCCTTGCCTC CGGGCAGCGG CGAGGTGATG
    GAACTTAATC GATCCGAGAG CTTGTGTCAT GAAAATTCAT TCTTCATTAA TGAAGCTTTA
    GTACAAATGT CACTTAACAC TTACAACAAA TCAACTCCAT CAGGTGTCCA CCAGGAAGCC
    GCTTATCCCC AGAATATAGC TAATTCGGAC TTGGTAAGGA CTAGTGAAAG TCCCGTAGAC
    CTCCAGTTGG ACATTTCTCA GCCCTTGGAT GTCAACCACG AACTGTGGAC AGCCGCTGGG
    TCCTGTCATC AGATACCCCC TCTCCACGAA AGCAGCAAGG ATTCTTGTAA AAATGGAAGA
    ATTTACATCA GTCAGCTATG TTTCCAGAGC CATTCGTCAG AAATGTTTTG GCGACCAGAC
    TTGACCATCT CAGCTTGGGA TAGAGAGCTT CCGAATCTGC AGAACTTCCA ACCGCTGTTG
    GTTGCATTAT GTAGATGGTT GCGTTACGTC CGTACATCCC CGGGAGATCT TGCCGTCATC
    CTCGAGTCCC CTGAGCAGGT TTTTCAGCTA GAAAGAAAAT GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013964557.1
    CDS195..4559
    Translation

    Target ORF information:

    RefSeq Version XM_014109082.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 28 (ARHGEF28), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109082.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGACTCGG ACTCGGACTC CCCTTTGAAC TACTCGTGGC CGTCCTTCCC CAAGATGAAG 
    ACGCGCCGGA GGGCGGCCAA GCCAGATCAC TCTTTCGATG TCTTCAAAAA ACCTAAGCAT
    CCCCCAGCGT TCTTTGCTGC AGGCGGGCTC TCTGATGTGC TGAATGGCGG CGATGAAGTT
    TACGCCAACT GTATGGTGAT TGACCAGGTT GGTGATTTGG ATATTAACTA CATCAATTTA
    GAAGGAATCT CTACAGACGC CTGTGCTGAA TCCATGGGTT CTACTCTGGG GCCTCAGAAC
    TGCGAGCATA TCCTTGCTGC TGAAACCAGC CATGGGAAAT ACCCACTAAG TGAGAACAAG
    GAAGTGGTCT CAGATACCGA AGCTCAGCAG TCCTTCCCAT CACCGTCTAG TTCATATGCT
    TCCAATTTTG ATCTTTCCTT TGGTCTTCAT GGATTTGAGA AGGAACAAAG TCTCCTGAAG
    AAAAGAAGCT CCAGCCTGGA TGCCTTGGAT GCCGACAGCG AAGGGGAAGG GCATTCTGAG
    CGGTCACACA TCTGCTACAC GCCAGCATCT CAGAGTTCCT CGAGGACTGG GATTCCCAGT
    GGGGATGAGC TGGACTCCTT CGAGACCGCC ACCAAACCTG ATTTTAGTAT CTCCAGGACA
    GAGTCGCTTT CTTTATCAAG TAGCCTGCAG TTGAAGGAAT CATTGCTTCC TGGAGTCCGC
    TCACGTTCTT ATTCCTGCTC ATCACCCAAA ATTTCTTTAG GAAAAACTCG ATTGGTGCGT
    GATTTCACAG TGTGCAATTC TGGTGAAGAG CAAAGAGCCT TCGGCTTCCC GGAGCCCGCC
    AGGGAGAGGA GGATTCAAGA GGAAGAATGG GATAAATACA TCATACCTGC CAAGTCAGAG
    TCTGAGAAAT ACAAAGTGAG CCGGACCTTC AGCTTCCTCA TGAACAGGAT GACAAGCCCT
    CGGAACAAAT CTAAGACGAA AAGCAAGGAT GCCAAAGACA AAGAGAAACC GAGCCGACAT
    CAGTTTGTCC CCGGAACCTT CTCTGGGGTT CTCCAGTGTT CCGTTTGTGA TAAAACGCTC
    CTGGGGAAGG AGTCATCCCA GTGTTCCAAC TGTAATGCAA ACGTCCACAA GGGTTGTAAG
    GACGCTGCAC CTCCGTGTAC CAAGAAATTC CAGGAGAAAT ATAACAAGAA CAAACCGCAG
    ACCATCCTTG GAAACTCTTC ATTTCGAGAC ATCCCGCAAC CCAGTCTCTC CTTGCACCCT
    TCTTCCTCCA TGCCTATCGG ATTGCCCACT GGGAGAAAAG AAGCTTCTGC CCAGGCCCAT
    CCCTTGTCCA GAAGTGTTCC AGGCACTGCC TTGGAAAGCT TCAGAAGATC GGGGCTATCC
    CTGGAGTCTG AGAGTGACGG GAACAGCTGG AGAAGCAGGT CTCATTCCGA TGAGCTGTTA
    CAGTCCATGG GCTCTTCTCC CTCCACGGAA TCCTTCTTAA TGGAAGATGT CGTGGACTCT
    TCTCTATGGA GTGACCTCAG CAGTGACGCC CAGGAGTTGG AAGCAGAATC CTGGAGTCTG
    GTGGTGGACC CCTCCTTTTG CAATAAGCAG GAGAAAGATG TCATCAAAAG ACAGGATGTC
    ATTTTCGAGC TTATGCAAAC AGAGATGCAT CATATCCAGA CCCTGCTCAT CATGTCTGAG
    ATCTTCAGGA AAGGAATGAA GGAGGAGCTG CAGCTTGATC ACAGCACCGT GGATAAAATC
    TTTCCCTGCT TAGATGAGTT ACTTGAAATC CACAGGCATT TCTTTTATAG TATGAAGGAG
    CGAAGGCAGG AGTCCTGTGC TGGTGGTGAC AGGAATTTTG TCATCAGCCG AATTGGAGAT
    ATTCTAGTAC AGCAGTTTTC AGAAGAAAAT GCAAATCAAA TGAAGAAAAT ATATGGAGAA
    TTCTGTAGCC ATCACAAAGA AGCTGTTAGC CTCTATAAAG AACTCCAACA GAATAAGAAG
    TTTCAGAATT TTATTAAGCT TCGAAATAGT AATCTTTTGG CTCGACGCCG AGGAATCCCA
    GAATGCATTC TGCTAGTCAC TCAGCGCATC ACAAAATACC CTGTCCTGGT GGAACGGATT
    TTGCAGTACA CAAAGGAAAG AAGTGAAGAA CATCAAGACT TGTGCAAAGC TCTGTGCTTA
    ATTAAAGACA TGATTGCAGC AGTGGATTTA AAAGTCAGCG AGTATGAGAA AAACCAAAAA
    TGGCTTGAGA TCCTAAATAA GATCGAAAAC AAAACCTACA CAAAGCTCAA AAATGGCCAC
    GTGTTTAGGA AGCAAGCACT GATAAGTAAA GAAAGGACTC TCTTATATGA TGGCCTTGTT
    TATTGGAAAA CTGCTACGGG TCGTTTCAAA GATATCCTGG CTCTACTTCT AACTGATGTG
    CTGCTCTTTT TACAAGAAAA AGACCAGAAA TACATCTTTG CAGCTGTTGA TCAGAAGCCA
    TCGGTTATTT CCCTTCAGAA GCTCATTGCT AGAGAAGTCG CCAACGAGGA AAGAGGAATG
    TTCCTGATCA GTGCATCCTC TGCTGGTCCT GAGATGTACG AAATTCACAC CAACTCCAAG
    GAGGAACGAA ATAACTGGAT GAGACGAATC CAACAGGCCG TGGAAAGTTG CCCAGAAGAA
    GAAGGGGGAA GGACAAGTGA ATCTGATGAA GAGAGGCGGA AAGCTGAAGC AAGAGTGGCC
    AAAATCCAGC AATGTCAAGA AATACTCAGT AACCAAGACC AACAGATTTG CGCATATTTG
    GAGGAGAAGC TGCATATCTA CGCTGAACTT GGAGAACTGA GTGGATTCAA GGATGTCCAT
    CTAGAGCCCC ACCTCCTCAT CAAACCTGAC CCAGGGGAGC CTCCCCAGGC AGCCTCATTA
    CTAGCAGCAG CCCTGAGAGA AGCGGAGAGC CTGCAGGTTG CCGTGAAGGC CTCACAAGCG
    GGTGATGCTC GCCCATCACC TGAGGAGGGT TCTGCAGAAC CTGCGGTTGC AGATGCACAG
    GGTCCTCCCG AGGAGCCAGG ATCATCTCCA GCCTCACTAG TCACAGAAGG GACAGGAGGA
    AGAGGCTGTT GGGATGTGGA TCCTGGCCCC CAGGGTGTGG GAACCGACTT GGCGGTCTCT
    GATGCAGGGG AGAAGAGTCT TAAAAATCTG TGCTGCAGCC GGCAAAGTCC CTGGGGTGTC
    AGTCACCATC ACCAGCTGCA GCCCTGCACA GGGACCACAA GGGGGAGGGC CAGCTCCACG
    AAAAGTAGGA CACTACACAG CTCAGGCTTG TATCAGGCTT CGTGGTTCTT GGGAGTGGAA
    TATAGAAGTT TCACGGTTTC TTCACAGTCA GAGATTATAC AAGCGATCCA GAATTTAACC
    CGACTCCTCT ATAGCCTTCA GGCCGCGCTG ACCATCCAGG ACAGCCACAT CCAGGTGCAC
    AGGCTGCTGC TGCAGCAGGC TGGCCTGCCG GCGTCCCAAG AGGAGCACGG CGAGCTGGCG
    AGGCAGCGGG AGGAGCTGGC CGCCCTGCCC GGGCTCCAGT GGCAGCTGCA GCAGGAGCGG
    CGGCGCGGCC AGCGCACCTG GGAGCGGCAG CAGCGCGAGC AGGAGGCCAG GGCCTGCCGG
    CTGCGGGAGC GCGAGCAGGG CTGCCGCGGC CGCGAGGAGC TGCTGCTCAG GGCCCGCGGG
    GAGCTGGACC GGCAGCTGCG GGAGCACCGG CAGGACCTGG AGCGGCTGCG GGAGGGCCAG
    CGCCTGGTGG AGAGTGCGAG GGCCCGCACG CGCGCCCAGC ACAGCCTGCT GCGTGGCCCG
    AGGCCCGGGC GCCAGAGCAG CCTGCCCGCG GCCTTGCCTC CGGGCAGCGG CGAGGTGATG
    GAACTTAATC GATCCGAGAG CTTGTGTCAT GAAAATTCAT TCTTCATTAA TGAAGCTTTA
    GTACAAATGT CACTTAACAC TTACAACAAA TCAACTCCAT CAGGTGTCCA CCAGGAAGCC
    GCTTATCCCC AGAATATAGC TAATTCGGAC TTGGTAAGGA CTAGTGAAAG TCCCGTAGAC
    CTCCAGTTGG ACATTTCTCA GCCCTTGGAT GTCAACCACG AACTGTGGAC AGCCGCTGGG
    TCCTGTCATC AGATACCCCC TCTCCACGAA AGCAGCAAGG ATTCTTGTAA AAATGGAAGA
    ATTTACATCA GTCAGCTATG TTTCCAGAGC CATTCGTCAG AAATGTTTTG GCGACCAGAC
    TTGACCATCT CAGCTTGGGA TAGAGAGCTT CCGAATCTGC AGAACTTCCA ACCGCTGTTG
    GTTGCATTAT GTAGATGGTT GCGTTACGTC CGTACATCCC CGGGAGATCT TGCCGTCATC
    CTCGAGTCCC CTGAGCAGGT TTTTCAGCTA GAAAGAAAAT GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35388
    Clone ID Related Accession (Same CDS sequence) XM_014109082.1 , XM_014109082.2
    Accession Version XM_014109082.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4365bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rho guanine nucleotide exchange factor 28 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109082.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGACTCGG ACTCGGACTC CCCTTTGAAC TACTCGTGGC CGTCCTTCCC CAAGATGAAG 
    ACGCGCCGGA GGGCGGCCAA GCCAGATCAC TCTTTCGATG TCTTCAAAAA ACCTAAGCAT
    CCCCCAGCGT TCTTTGCTGC AGGCGGGCTC TCTGATGTGC TGAATGGCGG CGATGAAGTT
    TACGCCAACT GTATGGTGAT TGACCAGGTT GGTGATTTGG ATATTAACTA CATCAATTTA
    GAAGGAATCT CTACAGACGC CTGTGCTGAA TCCATGGGTT CTACTCTGGG GCCTCAGAAC
    TGCGAGCATA TCCTTGCTGC TGAAACCAGC CATGGGAAAT ACCCACTAAG TGAGAACAAG
    GAAGTGGTCT CAGATACCGA AGCTCAGCAG TCCTTCCCAT CACCGTCTAG TTCATATGCT
    TCCAATTTTG ATCTTTCCTT TGGTCTTCAT GGATTTGAGA AGGAACAAAG TCTCCTGAAG
    AAAAGAAGCT CCAGCCTGGA TGCCTTGGAT GCCGACAGCG AAGGGGAAGG GCATTCTGAG
    CGGTCACACA TCTGCTACAC GCCAGCATCT CAGAGTTCCT CGAGGACTGG GATTCCCAGT
    GGGGATGAGC TGGACTCCTT CGAGACCGCC ACCAAACCTG ATTTTAGTAT CTCCAGGACA
    GAGTCGCTTT CTTTATCAAG TAGCCTGCAG TTGAAGGAAT CATTGCTTCC TGGAGTCCGC
    TCACGTTCTT ATTCCTGCTC ATCACCCAAA ATTTCTTTAG GAAAAACTCG ATTGGTGCGT
    GATTTCACAG TGTGCAATTC TGGTGAAGAG CAAAGAGCCT TCGGCTTCCC GGAGCCCGCC
    AGGGAGAGGA GGATTCAAGA GGAAGAATGG GATAAATACA TCATACCTGC CAAGTCAGAG
    TCTGAGAAAT ACAAAGTGAG CCGGACCTTC AGCTTCCTCA TGAACAGGAT GACAAGCCCT
    CGGAACAAAT CTAAGACGAA AAGCAAGGAT GCCAAAGACA AAGAGAAACC GAGCCGACAT
    CAGTTTGTCC CCGGAACCTT CTCTGGGGTT CTCCAGTGTT CCGTTTGTGA TAAAACGCTC
    CTGGGGAAGG AGTCATCCCA GTGTTCCAAC TGTAATGCAA ACGTCCACAA GGGTTGTAAG
    GACGCTGCAC CTCCGTGTAC CAAGAAATTC CAGGAGAAAT ATAACAAGAA CAAACCGCAG
    ACCATCCTTG GAAACTCTTC ATTTCGAGAC ATCCCGCAAC CCAGTCTCTC CTTGCACCCT
    TCTTCCTCCA TGCCTATCGG ATTGCCCACT GGGAGAAAAG AAGCTTCTGC CCAGGCCCAT
    CCCTTGTCCA GAAGTGTTCC AGGCACTGCC TTGGAAAGCT TCAGAAGATC GGGGCTATCC
    CTGGAGTCTG AGAGTGACGG GAACAGCTGG AGAAGCAGGT CTCATTCCGA TGAGCTGTTA
    CAGTCCATGG GCTCTTCTCC CTCCACGGAA TCCTTCTTAA TGGAAGATGT CGTGGACTCT
    TCTCTATGGA GTGACCTCAG CAGTGACGCC CAGGAGTTGG AAGCAGAATC CTGGAGTCTG
    GTGGTGGACC CCTCCTTTTG CAATAAGCAG GAGAAAGATG TCATCAAAAG ACAGGATGTC
    ATTTTCGAGC TTATGCAAAC AGAGATGCAT CATATCCAGA CCCTGCTCAT CATGTCTGAG
    ATCTTCAGGA AAGGAATGAA GGAGGAGCTG CAGCTTGATC ACAGCACCGT GGATAAAATC
    TTTCCCTGCT TAGATGAGTT ACTTGAAATC CACAGGCATT TCTTTTATAG TATGAAGGAG
    CGAAGGCAGG AGTCCTGTGC TGGTGGTGAC AGGAATTTTG TCATCAGCCG AATTGGAGAT
    ATTCTAGTAC AGCAGTTTTC AGAAGAAAAT GCAAATCAAA TGAAGAAAAT ATATGGAGAA
    TTCTGTAGCC ATCACAAAGA AGCTGTTAGC CTCTATAAAG AACTCCAACA GAATAAGAAG
    TTTCAGAATT TTATTAAGCT TCGAAATAGT AATCTTTTGG CTCGACGCCG AGGAATCCCA
    GAATGCATTC TGCTAGTCAC TCAGCGCATC ACAAAATACC CTGTCCTGGT GGAACGGATT
    TTGCAGTACA CAAAGGAAAG AAGTGAAGAA CATCAAGACT TGTGCAAAGC TCTGTGCTTA
    ATTAAAGACA TGATTGCAGC AGTGGATTTA AAAGTCAGCG AGTATGAGAA AAACCAAAAA
    TGGCTTGAGA TCCTAAATAA GATCGAAAAC AAAACCTACA CAAAGCTCAA AAATGGCCAC
    GTGTTTAGGA AGCAAGCACT GATAAGTAAA GAAAGGACTC TCTTATATGA TGGCCTTGTT
    TATTGGAAAA CTGCTACGGG TCGTTTCAAA GATATCCTGG CTCTACTTCT AACTGATGTG
    CTGCTCTTTT TACAAGAAAA AGACCAGAAA TACATCTTTG CAGCTGTTGA TCAGAAGCCA
    TCGGTTATTT CCCTTCAGAA GCTCATTGCT AGAGAAGTCG CCAACGAGGA AAGAGGAATG
    TTCCTGATCA GTGCATCCTC TGCTGGTCCT GAGATGTACG AAATTCACAC CAACTCCAAG
    GAGGAACGAA ATAACTGGAT GAGACGAATC CAACAGGCCG TGGAAAGTTG CCCAGAAGAA
    GAAGGGGGAA GGACAAGTGA ATCTGATGAA GAGAGGCGGA AAGCTGAAGC AAGAGTGGCC
    AAAATCCAGC AATGTCAAGA AATACTCAGT AACCAAGACC AACAGATTTG CGCATATTTG
    GAGGAGAAGC TGCATATCTA CGCTGAACTT GGAGAACTGA GTGGATTCAA GGATGTCCAT
    CTAGAGCCCC ACCTCCTCAT CAAACCTGAC CCAGGGGAGC CTCCCCAGGC AGCCTCATTA
    CTAGCAGCAG CCCTGAGAGA AGCGGAGAGC CTGCAGGTTG CCGTGAAGGC CTCACAAGCG
    GGTGATGCTC GCCCATCACC TGAGGAGGGT TCTGCAGAAC CTGCGGTTGC AGATGCACAG
    GGTCCTCCCG AGGAGCCAGG ATCATCTCCA GCCTCACTAG TCACAGAAGG GACAGGAGGA
    AGAGGCTGTT GGGATGTGGA TCCTGGCCCC CAGGGTGTGG GAACCGACTT GGCGGTCTCT
    GATGCAGGGG AGAAGAGTCT TAAAAATCTG TGCTGCAGCC GGCAAAGTCC CTGGGGTGTC
    AGTCACCATC ACCAGCTGCA GCCCTGCACA GGGACCACAA GGGGGAGGGC CAGCTCCACG
    AAAAGTAGGA CACTACACAG CTCAGGCTTG TATCAGGCTT CGTGGTTCTT GGGAGTGGAA
    TATAGAAGTT TCACGGTTTC TTCACAGTCA GAGATTATAC AAGCGATCCA GAATTTAACC
    CGACTCCTCT ATAGCCTTCA GGCCGCGCTG ACCATCCAGG ACAGCCACAT CCAGGTGCAC
    AGGCTGCTGC TGCAGCAGGC TGGCCTGCCG GCGTCCCAAG AGGAGCACGG CGAGCTGGCG
    AGGCAGCGGG AGGAGCTGGC CGCCCTGCCC GGGCTCCAGT GGCAGCTGCA GCAGGAGCGG
    CGGCGCGGCC AGCGCACCTG GGAGCGGCAG CAGCGCGAGC AGGAGGCCAG GGCCTGCCGG
    CTGCGGGAGC GCGAGCAGGG CTGCCGCGGC CGCGAGGAGC TGCTGCTCAG GGCCCGCGGG
    GAGCTGGACC GGCAGCTGCG GGAGCACCGG CAGGACCTGG AGCGGCTGCG GGAGGGCCAG
    CGCCTGGTGG AGAGTGCGAG GGCCCGCACG CGCGCCCAGC ACAGCCTGCT GCGTGGCCCG
    AGGCCCGGGC GCCAGAGCAG CCTGCCCGCG GCCTTGCCTC CGGGCAGCGG CGAGGTGATG
    GAACTTAATC GATCCGAGAG CTTGTGTCAT GAAAATTCAT TCTTCATTAA TGAAGCTTTA
    GTACAAATGT CACTTAACAC TTACAACAAA TCAACTCCAT CAGGTGTCCA CCAGGAAGCC
    GCTTATCCCC AGAATATAGC TAATTCGGAC TTGGTAAGGA CTAGTGAAAG TCCCGTAGAC
    CTCCAGTTGG ACATTTCTCA GCCCTTGGAT GTCAACCACG AACTGTGGAC AGCCGCTGGG
    TCCTGTCATC AGATACCCCC TCTCCACGAA AGCAGCAAGG ATTCTTGTAA AAATGGAAGA
    ATTTACATCA GTCAGCTATG TTTCCAGAGC CATTCGTCAG AAATGTTTTG GCGACCAGAC
    TTGACCATCT CAGCTTGGGA TAGAGAGCTT CCGAATCTGC AGAACTTCCA ACCGCTGTTG
    GTTGCATTAT GTAGATGGTT GCGTTACGTC CGTACATCCC CGGGAGATCT TGCCGTCATC
    CTCGAGTCCC CTGAGCAGGT TTTTCAGCTA GAAAGAAAAT GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013964557.1
    CDS157..4521
    Translation

    Target ORF information:

    RefSeq Version XM_014109082.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109082.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGACTCGG ACTCGGACTC CCCTTTGAAC TACTCGTGGC CGTCCTTCCC CAAGATGAAG 
    ACGCGCCGGA GGGCGGCCAA GCCAGATCAC TCTTTCGATG TCTTCAAAAA ACCTAAGCAT
    CCCCCAGCGT TCTTTGCTGC AGGCGGGCTC TCTGATGTGC TGAATGGCGG CGATGAAGTT
    TACGCCAACT GTATGGTGAT TGACCAGGTT GGTGATTTGG ATATTAACTA CATCAATTTA
    GAAGGAATCT CTACAGACGC CTGTGCTGAA TCCATGGGTT CTACTCTGGG GCCTCAGAAC
    TGCGAGCATA TCCTTGCTGC TGAAACCAGC CATGGGAAAT ACCCACTAAG TGAGAACAAG
    GAAGTGGTCT CAGATACCGA AGCTCAGCAG TCCTTCCCAT CACCGTCTAG TTCATATGCT
    TCCAATTTTG ATCTTTCCTT TGGTCTTCAT GGATTTGAGA AGGAACAAAG TCTCCTGAAG
    AAAAGAAGCT CCAGCCTGGA TGCCTTGGAT GCCGACAGCG AAGGGGAAGG GCATTCTGAG
    CGGTCACACA TCTGCTACAC GCCAGCATCT CAGAGTTCCT CGAGGACTGG GATTCCCAGT
    GGGGATGAGC TGGACTCCTT CGAGACCGCC ACCAAACCTG ATTTTAGTAT CTCCAGGACA
    GAGTCGCTTT CTTTATCAAG TAGCCTGCAG TTGAAGGAAT CATTGCTTCC TGGAGTCCGC
    TCACGTTCTT ATTCCTGCTC ATCACCCAAA ATTTCTTTAG GAAAAACTCG ATTGGTGCGT
    GATTTCACAG TGTGCAATTC TGGTGAAGAG CAAAGAGCCT TCGGCTTCCC GGAGCCCGCC
    AGGGAGAGGA GGATTCAAGA GGAAGAATGG GATAAATACA TCATACCTGC CAAGTCAGAG
    TCTGAGAAAT ACAAAGTGAG CCGGACCTTC AGCTTCCTCA TGAACAGGAT GACAAGCCCT
    CGGAACAAAT CTAAGACGAA AAGCAAGGAT GCCAAAGACA AAGAGAAACC GAGCCGACAT
    CAGTTTGTCC CCGGAACCTT CTCTGGGGTT CTCCAGTGTT CCGTTTGTGA TAAAACGCTC
    CTGGGGAAGG AGTCATCCCA GTGTTCCAAC TGTAATGCAA ACGTCCACAA GGGTTGTAAG
    GACGCTGCAC CTCCGTGTAC CAAGAAATTC CAGGAGAAAT ATAACAAGAA CAAACCGCAG
    ACCATCCTTG GAAACTCTTC ATTTCGAGAC ATCCCGCAAC CCAGTCTCTC CTTGCACCCT
    TCTTCCTCCA TGCCTATCGG ATTGCCCACT GGGAGAAAAG AAGCTTCTGC CCAGGCCCAT
    CCCTTGTCCA GAAGTGTTCC AGGCACTGCC TTGGAAAGCT TCAGAAGATC GGGGCTATCC
    CTGGAGTCTG AGAGTGACGG GAACAGCTGG AGAAGCAGGT CTCATTCCGA TGAGCTGTTA
    CAGTCCATGG GCTCTTCTCC CTCCACGGAA TCCTTCTTAA TGGAAGATGT CGTGGACTCT
    TCTCTATGGA GTGACCTCAG CAGTGACGCC CAGGAGTTGG AAGCAGAATC CTGGAGTCTG
    GTGGTGGACC CCTCCTTTTG CAATAAGCAG GAGAAAGATG TCATCAAAAG ACAGGATGTC
    ATTTTCGAGC TTATGCAAAC AGAGATGCAT CATATCCAGA CCCTGCTCAT CATGTCTGAG
    ATCTTCAGGA AAGGAATGAA GGAGGAGCTG CAGCTTGATC ACAGCACCGT GGATAAAATC
    TTTCCCTGCT TAGATGAGTT ACTTGAAATC CACAGGCATT TCTTTTATAG TATGAAGGAG
    CGAAGGCAGG AGTCCTGTGC TGGTGGTGAC AGGAATTTTG TCATCAGCCG AATTGGAGAT
    ATTCTAGTAC AGCAGTTTTC AGAAGAAAAT GCAAATCAAA TGAAGAAAAT ATATGGAGAA
    TTCTGTAGCC ATCACAAAGA AGCTGTTAGC CTCTATAAAG AACTCCAACA GAATAAGAAG
    TTTCAGAATT TTATTAAGCT TCGAAATAGT AATCTTTTGG CTCGACGCCG AGGAATCCCA
    GAATGCATTC TGCTAGTCAC TCAGCGCATC ACAAAATACC CTGTCCTGGT GGAACGGATT
    TTGCAGTACA CAAAGGAAAG AAGTGAAGAA CATCAAGACT TGTGCAAAGC TCTGTGCTTA
    ATTAAAGACA TGATTGCAGC AGTGGATTTA AAAGTCAGCG AGTATGAGAA AAACCAAAAA
    TGGCTTGAGA TCCTAAATAA GATCGAAAAC AAAACCTACA CAAAGCTCAA AAATGGCCAC
    GTGTTTAGGA AGCAAGCACT GATAAGTAAA GAAAGGACTC TCTTATATGA TGGCCTTGTT
    TATTGGAAAA CTGCTACGGG TCGTTTCAAA GATATCCTGG CTCTACTTCT AACTGATGTG
    CTGCTCTTTT TACAAGAAAA AGACCAGAAA TACATCTTTG CAGCTGTTGA TCAGAAGCCA
    TCGGTTATTT CCCTTCAGAA GCTCATTGCT AGAGAAGTCG CCAACGAGGA AAGAGGAATG
    TTCCTGATCA GTGCATCCTC TGCTGGTCCT GAGATGTACG AAATTCACAC CAACTCCAAG
    GAGGAACGAA ATAACTGGAT GAGACGAATC CAACAGGCCG TGGAAAGTTG CCCAGAAGAA
    GAAGGGGGAA GGACAAGTGA ATCTGATGAA GAGAGGCGGA AAGCTGAAGC AAGAGTGGCC
    AAAATCCAGC AATGTCAAGA AATACTCAGT AACCAAGACC AACAGATTTG CGCATATTTG
    GAGGAGAAGC TGCATATCTA CGCTGAACTT GGAGAACTGA GTGGATTCAA GGATGTCCAT
    CTAGAGCCCC ACCTCCTCAT CAAACCTGAC CCAGGGGAGC CTCCCCAGGC AGCCTCATTA
    CTAGCAGCAG CCCTGAGAGA AGCGGAGAGC CTGCAGGTTG CCGTGAAGGC CTCACAAGCG
    GGTGATGCTC GCCCATCACC TGAGGAGGGT TCTGCAGAAC CTGCGGTTGC AGATGCACAG
    GGTCCTCCCG AGGAGCCAGG ATCATCTCCA GCCTCACTAG TCACAGAAGG GACAGGAGGA
    AGAGGCTGTT GGGATGTGGA TCCTGGCCCC CAGGGTGTGG GAACCGACTT GGCGGTCTCT
    GATGCAGGGG AGAAGAGTCT TAAAAATCTG TGCTGCAGCC GGCAAAGTCC CTGGGGTGTC
    AGTCACCATC ACCAGCTGCA GCCCTGCACA GGGACCACAA GGGGGAGGGC CAGCTCCACG
    AAAAGTAGGA CACTACACAG CTCAGGCTTG TATCAGGCTT CGTGGTTCTT GGGAGTGGAA
    TATAGAAGTT TCACGGTTTC TTCACAGTCA GAGATTATAC AAGCGATCCA GAATTTAACC
    CGACTCCTCT ATAGCCTTCA GGCCGCGCTG ACCATCCAGG ACAGCCACAT CCAGGTGCAC
    AGGCTGCTGC TGCAGCAGGC TGGCCTGCCG GCGTCCCAAG AGGAGCACGG CGAGCTGGCG
    AGGCAGCGGG AGGAGCTGGC CGCCCTGCCC GGGCTCCAGT GGCAGCTGCA GCAGGAGCGG
    CGGCGCGGCC AGCGCACCTG GGAGCGGCAG CAGCGCGAGC AGGAGGCCAG GGCCTGCCGG
    CTGCGGGAGC GCGAGCAGGG CTGCCGCGGC CGCGAGGAGC TGCTGCTCAG GGCCCGCGGG
    GAGCTGGACC GGCAGCTGCG GGAGCACCGG CAGGACCTGG AGCGGCTGCG GGAGGGCCAG
    CGCCTGGTGG AGAGTGCGAG GGCCCGCACG CGCGCCCAGC ACAGCCTGCT GCGTGGCCCG
    AGGCCCGGGC GCCAGAGCAG CCTGCCCGCG GCCTTGCCTC CGGGCAGCGG CGAGGTGATG
    GAACTTAATC GATCCGAGAG CTTGTGTCAT GAAAATTCAT TCTTCATTAA TGAAGCTTTA
    GTACAAATGT CACTTAACAC TTACAACAAA TCAACTCCAT CAGGTGTCCA CCAGGAAGCC
    GCTTATCCCC AGAATATAGC TAATTCGGAC TTGGTAAGGA CTAGTGAAAG TCCCGTAGAC
    CTCCAGTTGG ACATTTCTCA GCCCTTGGAT GTCAACCACG AACTGTGGAC AGCCGCTGGG
    TCCTGTCATC AGATACCCCC TCTCCACGAA AGCAGCAAGG ATTCTTGTAA AAATGGAAGA
    ATTTACATCA GTCAGCTATG TTTCCAGAGC CATTCGTCAG AAATGTTTTG GCGACCAGAC
    TTGACCATCT CAGCTTGGGA TAGAGAGCTT CCGAATCTGC AGAACTTCCA ACCGCTGTTG
    GTTGCATTAT GTAGATGGTT GCGTTACGTC CGTACATCCC CGGGAGATCT TGCCGTCATC
    CTCGAGTCCC CTGAGCAGGT TTTTCAGCTA GAAAGAAAAT GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.