GBF1 cDNA ORF clone, Canis lupus familiaris(dog)

The following GBF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GBF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe43951 XM_014108957.2
Latest version!
Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe43952 XM_014108958.2
Latest version!
Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe27947 XM_005637657.3
Latest version!
Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe27948 XM_005637658.3
Latest version!
Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe27949 XM_005637659.3
Latest version!
Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe27950 XM_005637660.3
Latest version!
Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe27951 XM_005637661.3
Latest version!
Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe27952 XM_543987.6
Latest version!
Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
View Old Accession Versions >>
OCe27951 XM_005637661.2 Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe27952 XM_543987.5 Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe27947 XM_005637657.2 Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe27948 XM_005637658.2 Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe27950 XM_005637660.2 Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe43952 XM_014108958.1 Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe27949 XM_005637659.2 Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe43951 XM_014108957.1 Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe43951
    Clone ID Related Accession (Same CDS sequence) XM_014108957.1 , XM_014108957.2
    Accession Version XM_014108957.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5586bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726109.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
    AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA
    CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT
    CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC
    ATGAGTGATT CATCCAAGTG GAAGAAACAG AAGAGATCCC CCCGGCCCCT GCGCCATATG
    ACCAAAGTAA CATCAGGTTC AGAGTTGCCC ACCTCCAATG GAACCACCTT ATCCTCTAAC
    CTCACTGGGG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAA CGGTGGTCAG CCCCTCTACA GACAGTGGCC TGGAGTTCTC CTCTCAGACC
    ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAACCTGGCT CTCCAGGGTA TAGCACAGCT
    ACAGAGCCTG GTAGCAGTGA GCTAGGGGTT CCTGAGCAGC CTGACCCGCA GGAAGGAGTC
    CATGTGGAAA AGGCCCAGTC AGCATCTGTG GAATCCATCC CCGAAGTGTT AGAGGAGTGC
    ACGTCTCCTG CTGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC
    CGGGGTGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGTA CAGCTTTGGT TCCCTATGGT
    CTTCCCTGCA TCCGTGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACATGACCGC
    CACAATTCAG AAGTTATGAT CCACATGGGA CTGCATTTGC TGACAGTGGC TCTTGAATCT
    GCCCCTGTAG CCCAGTGCCA AACCCTTTTG GGCCTCATCA AAGATGAGAT GTGCCGCCAC
    TTACTCCAGC TACTCAGTGT AGAGCGACTC AACCTTTATG CTGCTTCCCT GCGGGTATGC
    TTCCTGCTTT TTGAGAGCAT GAGGGAACAT CTCAAGTTCC AATTGGAGAT GTACATCAAA
    AAGCTCATGG AGATCATCAC TGTGGAGAAC CCCAAAATGC CTTATGAGAT GAAAGAGATG
    GCACTGGAGG CCATTGTGCA GCTCTGGCAC ATCCCCAGTT TTGTCACTGA GCTCTATATC
    AACTATGATT GTGACTACTA CTGTTCAAAC CTCTTTGAAG ACCTCACCAA GCTGCTGTCC
    AAGAATGCCT TCCCTGTGTC TGGTCAGCTC TATACAACAC ACCTCCTGTC TCTTGATGCC
    CTGTTGACAG TGATTGACAG CACTGAGGCC CATTGCCAGG CCAAAGTCCT CAACAGTCTT
    ACCCAGCAAG AGAAGAAAGA AGCAGCCAGA TCTGGCTATG AGGCGGTGGA TGGTACCCGA
    GAATCCAGCA ATAGTGAGAG AGCAGCCAGC GATGGGAAAC CTGTAGGCAT AGCCGCAGAC
    ATCCCAGGTC TACATCTGCC AGGTGGAGGG CGGCTGCCAG CAGAACATGG GAAGCCAGGA
    TGCAGTGATC TGGAGGAAGC TGCTGACTCT GGGGCTGACA AAAAGTTTAC CCGGAAGCCA
    CCTCGATTTT CCTGTCTCCT GCCAGATCCA CGGGAACTGA TTGAAATTAA AAACAAAAAG
    AAGCTGCTAA TCACTGGCAC AGAGCAGTTC AACCAGAAAC CAAAGAAGGG AATCCAGTTT
    CTGCAGGAGA AAGGCCTCCT CACCATCCCA ATGGACAACA CAGAGGTAGC CCAGTGGCTC
    CGAGAGAACC CTCGGCTGGA CAAGAAAATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC
    ATTGACCTGT TGGAGAGCTT TGTGAGCACC TTCAGCTTTC AGGGTCTGCG GCTGGATGAA
    GCTCTTCGTC TCTACCTGGA AGCCTTTCGC TTACCTGGGG AAGCACCAGT CATCCAGAGG
    TTGCTGGAGG CATTCACAGA GCATTGGAGG AATTGTAATG GCTCCCCATT TGCCAATAGC
    GATGCCTGCT TTGCTCTGGC CTATGCTGTC ATCTTGCTTA ATACTGACCA GCACAACCAC
    AACGTTCGCA AACAGAATGC ACCCATGACT CTAGAGGAGT TTCGCAAAAA CCTAAAAGGT
    GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAGG ACATGTACCA TGCCATCAAG
    AATGAGGAAA TTGTGATGCC TGAAGAGCAG ACAGGCTTGG TTCGGGAGAA CTATGTGTGG
    AATGTGCTAC TTCATCGAGG TGCCACCCCA GAGGGCATAT TCCTTCGTGT GCCTGCTGGC
    AGCTATGATC TTGACCTCTT CACCATGACG TGGGGCCCCA CTATTGCTGC CCTTTCTTAT
    GTCTTTGACA AAAGCCTTGA GGAAACAATT ATCCAGAAAG CCATCTCAGG CTTCAGGAAG
    TGCGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATATCT
    CTGTGCAAAT TCACAGCTCT TAGCAGTGAG TCTATTGAGA ATCTGCCCAG TGTGTTTGGA
    AGCAACCCCA AAGCCCACAT TGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT
    GATATCCTGC GGGAGGGCTG GAAGAATATC ATGGAAGCCA TGCTGCAGCT CTTCCGAGCC
    CAACTGCTTC CCAAAGCTAT GGTGGAGGTA GAAGATTTTG TGGATCCCAA TGGCAAGATC
    TCTCTACAGA GGGAGGAGAC ACCATCAAAC CGAGGAGAGT CGACAGTGCT AAGCTTTGTG
    AGCTGGCTAA CACTAAGTGG TACTGAGCAG TCTAGTGTGC GGGGCCCATC CACTGAGAAC
    CAAGAGGCCA AAAGAGCGGC CTTGGACTGC ATCAAGCATT GTGACCCAGA AAAGATGATC
    ACAGAAAGCA AGTTCCTTCA GCTGGAGTCA CTGCAGGAGC TCATGAAGGC TCTGGTCTCA
    GTGACGCCAG ATGAAGAGAC ATATGATGAG GAGGATGCTG CTTTCTGCCT AGAGATGCTG
    CTGAGGATTG TGCTGGAGAA CAGGGACCGT GTGGGTTGTG TGTGGCAGAC TGTCCGAGAC
    CATCTATATC ACCTGTGTGT CCAGGCACAG GATTTCTGCT TCCTTGTGGA GCGGGCAGTG
    GTGGGGCTGC TCCGCTTGGC CATTCGACTA CTCCGAAGAG AAGAGATCAG TGGCCAGGTG
    CTGCTCTCCC TGCGTATTTT GCTACTAATG AAGCCCAGCG TGCTGTCCCG AGTCAGTCAC
    CAGGTAGCCT ATGGGCTCCA TGAACTTCTT AAGACCAACG CAGCCAACAT CCACTCAGGT
    GACGACTGGG CCACTCTCTT CACACTGCTG GAATGCATTG GCTCAGGTGT AAAGCCTCCA
    GCTGCCCTGC AGGCCACAGC CAGGGCTGAA GCACCTGATG CCGGGGCCCA ATCAGACAGC
    GAACTCCCAT CCTACCATCA GAATGATGTG AGCCTGGACC GAGGATACAC TTCTGACTCG
    GAGGTCTATG CTGACCATTG CAGGCCTGGC AAGATCCACC GATCAGCCAC TGATGCTGAT
    GTAGTCAACA GCGGCTGGTT AGTGGTGGGG AAGGATGACA TTGATAACTC CAAGCCAGGT
    CCTGGGCCCA GCCGGCTAGG CCCTTCACCC CTGGTCAATC AGTACAGCCT AACAGTGGGG
    CTGGACCTCG GGCCACACGA CACTAAGTCC CTGCTTAAAT GTGTAGAATC TCTGTCCTTC
    ATTGTGCGTG ATGCTGCCCA CATTACACCT GACAACTTTG AGCTCTGCGT GAAGACTCTC
    CGTATCTTTG TGGAGGCCAG TCTGAATGGC GGGTGCAAGT CCCAGGAGAA ACGTGGCAAG
    AGTCACAAAT ATGACAGCAA AGGAAACCGC TTCAAGAAGA AATCCAAGGA AGGTTCAGTG
    CTTCGGCGGC CTCGAACCTC TGGCCAACAT GCCACTCGGG GTGGGCATAG TGACGATGAT
    GAGGATGAAG GGGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGGTCAG TCAGGACTTG
    TTAGATCTGA TGCACACCCT GCACACGCGA GCAGCCTCTA TCTACAGCTC TTGGGCGGAG
    GAACAGCGCC ACCTAGAGAC AGGTGGCCGG AAGATAGAAG CGGATTCACG TACCCTCTGG
    GCCCACTGCT GGTGTCCCTT ACTGCAGGGC ATTGCCTGCT TGTGCTGTGA TGCCCGGCGC
    CAGGTGCGGA TGCAAGCACT GACTTATCTG CAACGAGCAC TACTGGTACA TGATCTGCAG
    AAGCTAGATG CCCTGGAGTG GGAGTCCTGT TTTAACAAGG TGCTGTTTCC TCTACTGACC
    AAACTCTTGG AGAACATCAG CCCTGCAGAT GTGGGTGGGA TGGAGGAGAC TCGAATGAGG
    GCTTCCACAC TGCTGTCTAA GGTCTTCCTG CAGCACCTGT CTCCACTGCT GTCACTCTCT
    ACCTTTGCGG CCCTCTGGCT GACCATCCTG GACTTCATGG ACAAGTATAT GCATGCAGGA
    TCCAGTGACT TACTGTCAGA GGCCATCCCT GAGTCTCTGA AGAACATGCT CCTGGTGATG
    GACACAGCAG AGATTTTCCA CAATGCAGAT GCCCGAGGAG GCAGCCCCTC AGCCCTCTGG
    GAGATCACCT GGGAGCGCAT TGACTGTTTT CTGCCCCACC TACGAGATGA GCTCTTCAAG
    CAGACTGTCA TCCAGGACCC CATGCCACTG GAGCCACATG CCCAAAAAAC TCTGGCCTCA
    GCCCACATGA CTCCTGCTGC TGGAGACACA AGGACACCTG GCCATCCACC TCCCCCAGAG
    ATGCCTTCTG AGCTGGGGGC CTGTGACTTC GAGAAGCCTG AGGGCCCTCG ACCTGCCAAC
    AGCAGCTCTC CTGGATCACC AGTGGCATCT AGCCCCAACA GACTGAGCCC TACTTCAGAT
    GGGCCTCCTC CTCTGGCTCA GCCTCCACTG ATCCTGCAGC CCTTGGCCTC CCCATTGCAG
    GTGGGCGTAC CACCCATGAC TCTACCAATC ATCCTCAACC CTGCTCTCAT TGAGGCCACT
    TCACCAGTGC CCCTCCTGGC CACACCCCGC CCCACAGACC CAATGCCCAC CTCCGAGGTC
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013964432.1
    CDS251..5836
    Translation

    Target ORF information:

    RefSeq Version XM_014108957.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108957.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
    AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA
    CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT
    CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC
    ATGAGTGATT CATCCAAGTG GAAGAAACAG AAGAGATCCC CCCGGCCCCT GCGCCATATG
    ACCAAAGTAA CATCAGGTTC AGAGTTGCCC ACCTCCAATG GAACCACCTT ATCCTCTAAC
    CTCACTGGGG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAA CGGTGGTCAG CCCCTCTACA GACAGTGGCC TGGAGTTCTC CTCTCAGACC
    ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAACCTGGCT CTCCAGGGTA TAGCACAGCT
    ACAGAGCCTG GTAGCAGTGA GCTAGGGGTT CCTGAGCAGC CTGACCCGCA GGAAGGAGTC
    CATGTGGAAA AGGCCCAGTC AGCATCTGTG GAATCCATCC CCGAAGTGTT AGAGGAGTGC
    ACGTCTCCTG CTGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC
    CGGGGTGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGTA CAGCTTTGGT TCCCTATGGT
    CTTCCCTGCA TCCGTGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACATGACCGC
    CACAATTCAG AAGTTATGAT CCACATGGGA CTGCATTTGC TGACAGTGGC TCTTGAATCT
    GCCCCTGTAG CCCAGTGCCA AACCCTTTTG GGCCTCATCA AAGATGAGAT GTGCCGCCAC
    TTACTCCAGC TACTCAGTGT AGAGCGACTC AACCTTTATG CTGCTTCCCT GCGGGTATGC
    TTCCTGCTTT TTGAGAGCAT GAGGGAACAT CTCAAGTTCC AATTGGAGAT GTACATCAAA
    AAGCTCATGG AGATCATCAC TGTGGAGAAC CCCAAAATGC CTTATGAGAT GAAAGAGATG
    GCACTGGAGG CCATTGTGCA GCTCTGGCAC ATCCCCAGTT TTGTCACTGA GCTCTATATC
    AACTATGATT GTGACTACTA CTGTTCAAAC CTCTTTGAAG ACCTCACCAA GCTGCTGTCC
    AAGAATGCCT TCCCTGTGTC TGGTCAGCTC TATACAACAC ACCTCCTGTC TCTTGATGCC
    CTGTTGACAG TGATTGACAG CACTGAGGCC CATTGCCAGG CCAAAGTCCT CAACAGTCTT
    ACCCAGCAAG AGAAGAAAGA AGCAGCCAGA TCTGGCTATG AGGCGGTGGA TGGTACCCGA
    GAATCCAGCA ATAGTGAGAG AGCAGCCAGC GATGGGAAAC CTGTAGGCAT AGCCGCAGAC
    ATCCCAGGTC TACATCTGCC AGGTGGAGGG CGGCTGCCAG CAGAACATGG GAAGCCAGGA
    TGCAGTGATC TGGAGGAAGC TGCTGACTCT GGGGCTGACA AAAAGTTTAC CCGGAAGCCA
    CCTCGATTTT CCTGTCTCCT GCCAGATCCA CGGGAACTGA TTGAAATTAA AAACAAAAAG
    AAGCTGCTAA TCACTGGCAC AGAGCAGTTC AACCAGAAAC CAAAGAAGGG AATCCAGTTT
    CTGCAGGAGA AAGGCCTCCT CACCATCCCA ATGGACAACA CAGAGGTAGC CCAGTGGCTC
    CGAGAGAACC CTCGGCTGGA CAAGAAAATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC
    ATTGACCTGT TGGAGAGCTT TGTGAGCACC TTCAGCTTTC AGGGTCTGCG GCTGGATGAA
    GCTCTTCGTC TCTACCTGGA AGCCTTTCGC TTACCTGGGG AAGCACCAGT CATCCAGAGG
    TTGCTGGAGG CATTCACAGA GCATTGGAGG AATTGTAATG GCTCCCCATT TGCCAATAGC
    GATGCCTGCT TTGCTCTGGC CTATGCTGTC ATCTTGCTTA ATACTGACCA GCACAACCAC
    AACGTTCGCA AACAGAATGC ACCCATGACT CTAGAGGAGT TTCGCAAAAA CCTAAAAGGT
    GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAGG ACATGTACCA TGCCATCAAG
    AATGAGGAAA TTGTGATGCC TGAAGAGCAG ACAGGCTTGG TTCGGGAGAA CTATGTGTGG
    AATGTGCTAC TTCATCGAGG TGCCACCCCA GAGGGCATAT TCCTTCGTGT GCCTGCTGGC
    AGCTATGATC TTGACCTCTT CACCATGACG TGGGGCCCCA CTATTGCTGC CCTTTCTTAT
    GTCTTTGACA AAAGCCTTGA GGAAACAATT ATCCAGAAAG CCATCTCAGG CTTCAGGAAG
    TGCGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATATCT
    CTGTGCAAAT TCACAGCTCT TAGCAGTGAG TCTATTGAGA ATCTGCCCAG TGTGTTTGGA
    AGCAACCCCA AAGCCCACAT TGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT
    GATATCCTGC GGGAGGGCTG GAAGAATATC ATGGAAGCCA TGCTGCAGCT CTTCCGAGCC
    CAACTGCTTC CCAAAGCTAT GGTGGAGGTA GAAGATTTTG TGGATCCCAA TGGCAAGATC
    TCTCTACAGA GGGAGGAGAC ACCATCAAAC CGAGGAGAGT CGACAGTGCT AAGCTTTGTG
    AGCTGGCTAA CACTAAGTGG TACTGAGCAG TCTAGTGTGC GGGGCCCATC CACTGAGAAC
    CAAGAGGCCA AAAGAGCGGC CTTGGACTGC ATCAAGCATT GTGACCCAGA AAAGATGATC
    ACAGAAAGCA AGTTCCTTCA GCTGGAGTCA CTGCAGGAGC TCATGAAGGC TCTGGTCTCA
    GTGACGCCAG ATGAAGAGAC ATATGATGAG GAGGATGCTG CTTTCTGCCT AGAGATGCTG
    CTGAGGATTG TGCTGGAGAA CAGGGACCGT GTGGGTTGTG TGTGGCAGAC TGTCCGAGAC
    CATCTATATC ACCTGTGTGT CCAGGCACAG GATTTCTGCT TCCTTGTGGA GCGGGCAGTG
    GTGGGGCTGC TCCGCTTGGC CATTCGACTA CTCCGAAGAG AAGAGATCAG TGGCCAGGTG
    CTGCTCTCCC TGCGTATTTT GCTACTAATG AAGCCCAGCG TGCTGTCCCG AGTCAGTCAC
    CAGGTAGCCT ATGGGCTCCA TGAACTTCTT AAGACCAACG CAGCCAACAT CCACTCAGGT
    GACGACTGGG CCACTCTCTT CACACTGCTG GAATGCATTG GCTCAGGTGT AAAGCCTCCA
    GCTGCCCTGC AGGCCACAGC CAGGGCTGAA GCACCTGATG CCGGGGCCCA ATCAGACAGC
    GAACTCCCAT CCTACCATCA GAATGATGTG AGCCTGGACC GAGGATACAC TTCTGACTCG
    GAGGTCTATG CTGACCATTG CAGGCCTGGC AAGATCCACC GATCAGCCAC TGATGCTGAT
    GTAGTCAACA GCGGCTGGTT AGTGGTGGGG AAGGATGACA TTGATAACTC CAAGCCAGGT
    CCTGGGCCCA GCCGGCTAGG CCCTTCACCC CTGGTCAATC AGTACAGCCT AACAGTGGGG
    CTGGACCTCG GGCCACACGA CACTAAGTCC CTGCTTAAAT GTGTAGAATC TCTGTCCTTC
    ATTGTGCGTG ATGCTGCCCA CATTACACCT GACAACTTTG AGCTCTGCGT GAAGACTCTC
    CGTATCTTTG TGGAGGCCAG TCTGAATGGC GGGTGCAAGT CCCAGGAGAA ACGTGGCAAG
    AGTCACAAAT ATGACAGCAA AGGAAACCGC TTCAAGAAGA AATCCAAGGA AGGTTCAGTG
    CTTCGGCGGC CTCGAACCTC TGGCCAACAT GCCACTCGGG GTGGGCATAG TGACGATGAT
    GAGGATGAAG GGGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGGTCAG TCAGGACTTG
    TTAGATCTGA TGCACACCCT GCACACGCGA GCAGCCTCTA TCTACAGCTC TTGGGCGGAG
    GAACAGCGCC ACCTAGAGAC AGGTGGCCGG AAGATAGAAG CGGATTCACG TACCCTCTGG
    GCCCACTGCT GGTGTCCCTT ACTGCAGGGC ATTGCCTGCT TGTGCTGTGA TGCCCGGCGC
    CAGGTGCGGA TGCAAGCACT GACTTATCTG CAACGAGCAC TACTGGTACA TGATCTGCAG
    AAGCTAGATG CCCTGGAGTG GGAGTCCTGT TTTAACAAGG TGCTGTTTCC TCTACTGACC
    AAACTCTTGG AGAACATCAG CCCTGCAGAT GTGGGTGGGA TGGAGGAGAC TCGAATGAGG
    GCTTCCACAC TGCTGTCTAA GGTCTTCCTG CAGCACCTGT CTCCACTGCT GTCACTCTCT
    ACCTTTGCGG CCCTCTGGCT GACCATCCTG GACTTCATGG ACAAGTATAT GCATGCAGGA
    TCCAGTGACT TACTGTCAGA GGCCATCCCT GAGTCTCTGA AGAACATGCT CCTGGTGATG
    GACACAGCAG AGATTTTCCA CAATGCAGAT GCCCGAGGAG GCAGCCCCTC AGCCCTCTGG
    GAGATCACCT GGGAGCGCAT TGACTGTTTT CTGCCCCACC TACGAGATGA GCTCTTCAAG
    CAGACTGTCA TCCAGGACCC CATGCCACTG GAGCCACATG CCCAAAAAAC TCTGGCCTCA
    GCCCACATGA CTCCTGCTGC TGGAGACACA AGGACACCTG GCCATCCACC TCCCCCAGAG
    ATGCCTTCTG AGCTGGGGGC CTGTGACTTC GAGAAGCCTG AGGGCCCTCG ACCTGCCAAC
    AGCAGCTCTC CTGGATCACC AGTGGCATCT AGCCCCAACA GACTGAGCCC TACTTCAGAT
    GGGCCTCCTC CTCTGGCTCA GCCTCCACTG ATCCTGCAGC CCTTGGCCTC CCCATTGCAG
    GTGGGCGTAC CACCCATGAC TCTACCAATC ATCCTCAACC CTGCTCTCAT TGAGGCCACT
    TCACCAGTGC CCCTCCTGGC CACACCCCGC CCCACAGACC CAATGCCCAC CTCCGAGGTC
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe43951
    Clone ID Related Accession (Same CDS sequence) XM_014108957.1 , XM_014108957.2
    Accession Version XM_014108957.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5586bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006610.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014108957.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
    AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA
    CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT
    CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC
    ATGAGTGATT CATCCAAGTG GAAGAAACAG AAGAGATCCC CCCGGCCCCT GCGCCATATG
    ACCAAAGTAA CATCAGGTTC AGAGTTGCCC ACCTCCAATG GAACCACCTT ATCCTCTAAC
    CTCACTGGGG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAA CGGTGGTCAG CCCCTCTACA GACAGTGGCC TGGAGTTCTC CTCTCAGACC
    ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAACCTGGCT CTCCAGGGTA TAGCACAGCT
    ACAGAGCCTG GTAGCAGTGA GCTAGGGGTT CCTGAGCAGC CTGACCCGCA GGAAGGAGTC
    CATGTGGAAA AGGCCCAGTC AGCATCTGTG GAATCCATCC CCGAAGTGTT AGAGGAGTGC
    ACGTCTCCTG CTGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC
    CGGGGTGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGTA CAGCTTTGGT TCCCTATGGT
    CTTCCCTGCA TCCGTGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACATGACCGC
    CACAATTCAG AAGTTATGAT CCACATGGGA CTGCATTTGC TGACAGTGGC TCTTGAATCT
    GCCCCTGTAG CCCAGTGCCA AACCCTTTTG GGCCTCATCA AAGATGAGAT GTGCCGCCAC
    TTACTCCAGC TACTCAGTGT AGAGCGACTC AACCTTTATG CTGCTTCCCT GCGGGTATGC
    TTCCTGCTTT TTGAGAGCAT GAGGGAACAT CTCAAGTTCC AATTGGAGAT GTACATCAAA
    AAGCTCATGG AGATCATCAC TGTGGAGAAC CCCAAAATGC CTTATGAGAT GAAAGAGATG
    GCACTGGAGG CCATTGTGCA GCTCTGGCAC ATCCCCAGTT TTGTCACTGA GCTCTATATC
    AACTATGATT GTGACTACTA CTGTTCAAAC CTCTTTGAAG ACCTCACCAA GCTGCTGTCC
    AAGAATGCCT TCCCTGTGTC TGGTCAGCTC TATACAACAC ACCTCCTGTC TCTTGATGCC
    CTGTTGACAG TGATTGACAG CACTGAGGCC CATTGCCAGG CCAAAGTCCT CAACAGTCTT
    ACCCAGCAAG AGAAGAAAGA AGCAGCCAGA TCTGGCTATG AGGCGGTGGA TGGTACCCGA
    GAATCCAGCA ATAGTGAGAG AGCAGCCAGC GATGGGAAAC CTGTAGGCAT AGCCGCAGAC
    ATCCCAGGTC TACATCTGCC AGGTGGAGGG CGGCTGCCAG CAGAACATGG GAAGCCAGGA
    TGCAGTGATC TGGAGGAAGC TGCTGACTCT GGGGCTGACA AAAAGTTTAC CCGGAAGCCA
    CCTCGATTTT CCTGTCTCCT GCCAGATCCA CGGGAACTGA TTGAAATTAA AAACAAAAAG
    AAGCTGCTAA TCACTGGCAC AGAGCAGTTC AACCAGAAAC CAAAGAAGGG AATCCAGTTT
    CTGCAGGAGA AAGGCCTCCT CACCATCCCA ATGGACAACA CAGAGGTAGC CCAGTGGCTC
    CGAGAGAACC CTCGGCTGGA CAAGAAAATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC
    ATTGACCTGT TGGAGAGCTT TGTGAGCACC TTCAGCTTTC AGGGTCTGCG GCTGGATGAA
    GCTCTTCGTC TCTACCTGGA AGCCTTTCGC TTACCTGGGG AAGCACCAGT CATCCAGAGG
    TTGCTGGAGG CATTCACAGA GCATTGGAGG AATTGTAATG GCTCCCCATT TGCCAATAGC
    GATGCCTGCT TTGCTCTGGC CTATGCTGTC ATCTTGCTTA ATACTGACCA GCACAACCAC
    AACGTTCGCA AACAGAATGC ACCCATGACT CTAGAGGAGT TTCGCAAAAA CCTAAAAGGT
    GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAGG ACATGTACCA TGCCATCAAG
    AATGAGGAAA TTGTGATGCC TGAAGAGCAG ACAGGCTTGG TTCGGGAGAA CTATGTGTGG
    AATGTGCTAC TTCATCGAGG TGCCACCCCA GAGGGCATAT TCCTTCGTGT GCCTGCTGGC
    AGCTATGATC TTGACCTCTT CACCATGACG TGGGGCCCCA CTATTGCTGC CCTTTCTTAT
    GTCTTTGACA AAAGCCTTGA GGAAACAATT ATCCAGAAAG CCATCTCAGG CTTCAGGAAG
    TGCGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATATCT
    CTGTGCAAAT TCACAGCTCT TAGCAGTGAG TCTATTGAGA ATCTGCCCAG TGTGTTTGGA
    AGCAACCCCA AAGCCCACAT TGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT
    GATATCCTGC GGGAGGGCTG GAAGAATATC ATGGAAGCCA TGCTGCAGCT CTTCCGAGCC
    CAACTGCTTC CCAAAGCTAT GGTGGAGGTA GAAGATTTTG TGGATCCCAA TGGCAAGATC
    TCTCTACAGA GGGAGGAGAC ACCATCAAAC CGAGGAGAGT CGACAGTGCT AAGCTTTGTG
    AGCTGGCTAA CACTAAGTGG TACTGAGCAG TCTAGTGTGC GGGGCCCATC CACTGAGAAC
    CAAGAGGCCA AAAGAGCGGC CTTGGACTGC ATCAAGCATT GTGACCCAGA AAAGATGATC
    ACAGAAAGCA AGTTCCTTCA GCTGGAGTCA CTGCAGGAGC TCATGAAGGC TCTGGTCTCA
    GTGACGCCAG ATGAAGAGAC ATATGATGAG GAGGATGCTG CTTTCTGCCT AGAGATGCTG
    CTGAGGATTG TGCTGGAGAA CAGGGACCGT GTGGGTTGTG TGTGGCAGAC TGTCCGAGAC
    CATCTATATC ACCTGTGTGT CCAGGCACAG GATTTCTGCT TCCTTGTGGA GCGGGCAGTG
    GTGGGGCTGC TCCGCTTGGC CATTCGACTA CTCCGAAGAG AAGAGATCAG TGGCCAGGTG
    CTGCTCTCCC TGCGTATTTT GCTACTAATG AAGCCCAGCG TGCTGTCCCG AGTCAGTCAC
    CAGGTAGCCT ATGGGCTCCA TGAACTTCTT AAGACCAACG CAGCCAACAT CCACTCAGGT
    GACGACTGGG CCACTCTCTT CACACTGCTG GAATGCATTG GCTCAGGTGT AAAGCCTCCA
    GCTGCCCTGC AGGCCACAGC CAGGGCTGAA GCACCTGATG CCGGGGCCCA ATCAGACAGC
    GAACTCCCAT CCTACCATCA GAATGATGTG AGCCTGGACC GAGGATACAC TTCTGACTCG
    GAGGTCTATG CTGACCATTG CAGGCCTGGC AAGATCCACC GATCAGCCAC TGATGCTGAT
    GTAGTCAACA GCGGCTGGTT AGTGGTGGGG AAGGATGACA TTGATAACTC CAAGCCAGGT
    CCTGGGCCCA GCCGGCTAGG CCCTTCACCC CTGGTCAATC AGTACAGCCT AACAGTGGGG
    CTGGACCTCG GGCCACACGA CACTAAGTCC CTGCTTAAAT GTGTAGAATC TCTGTCCTTC
    ATTGTGCGTG ATGCTGCCCA CATTACACCT GACAACTTTG AGCTCTGCGT GAAGACTCTC
    CGTATCTTTG TGGAGGCCAG TCTGAATGGC GGGTGCAAGT CCCAGGAGAA ACGTGGCAAG
    AGTCACAAAT ATGACAGCAA AGGAAACCGC TTCAAGAAGA AATCCAAGGA AGGTTCAGTG
    CTTCGGCGGC CTCGAACCTC TGGCCAACAT GCCACTCGGG GTGGGCATAG TGACGATGAT
    GAGGATGAAG GGGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGGTCAG TCAGGACTTG
    TTAGATCTGA TGCACACCCT GCACACGCGA GCAGCCTCTA TCTACAGCTC TTGGGCGGAG
    GAACAGCGCC ACCTAGAGAC AGGTGGCCGG AAGATAGAAG CGGATTCACG TACCCTCTGG
    GCCCACTGCT GGTGTCCCTT ACTGCAGGGC ATTGCCTGCT TGTGCTGTGA TGCCCGGCGC
    CAGGTGCGGA TGCAAGCACT GACTTATCTG CAACGAGCAC TACTGGTACA TGATCTGCAG
    AAGCTAGATG CCCTGGAGTG GGAGTCCTGT TTTAACAAGG TGCTGTTTCC TCTACTGACC
    AAACTCTTGG AGAACATCAG CCCTGCAGAT GTGGGTGGGA TGGAGGAGAC TCGAATGAGG
    GCTTCCACAC TGCTGTCTAA GGTCTTCCTG CAGCACCTGT CTCCACTGCT GTCACTCTCT
    ACCTTTGCGG CCCTCTGGCT GACCATCCTG GACTTCATGG ACAAGTATAT GCATGCAGGA
    TCCAGTGACT TACTGTCAGA GGCCATCCCT GAGTCTCTGA AGAACATGCT CCTGGTGATG
    GACACAGCAG AGATTTTCCA CAATGCAGAT GCCCGAGGAG GCAGCCCCTC AGCCCTCTGG
    GAGATCACCT GGGAGCGCAT TGACTGTTTT CTGCCCCACC TACGAGATGA GCTCTTCAAG
    CAGACTGTCA TCCAGGACCC CATGCCACTG GAGCCACATG CCCAAAAAAC TCTGGCCTCA
    GCCCACATGA CTCCTGCTGC TGGAGACACA AGGACACCTG GCCATCCACC TCCCCCAGAG
    ATGCCTTCTG AGCTGGGGGC CTGTGACTTC GAGAAGCCTG AGGGCCCTCG ACCTGCCAAC
    AGCAGCTCTC CTGGATCACC AGTGGCATCT AGCCCCAACA GACTGAGCCC TACTTCAGAT
    GGGCCTCCTC CTCTGGCTCA GCCTCCACTG ATCCTGCAGC CCTTGGCCTC CCCATTGCAG
    GTGGGCGTAC CACCCATGAC TCTACCAATC ATCCTCAACC CTGCTCTCAT TGAGGCCACT
    TCACCAGTGC CCCTCCTGGC CACACCCCGC CCCACAGACC CAATGCCCAC CTCCGAGGTC
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013964432.1
    CDS269..5854
    Translation

    Target ORF information:

    RefSeq Version XM_014108957.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108957.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
    AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA
    CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT
    CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC
    ATGAGTGATT CATCCAAGTG GAAGAAACAG AAGAGATCCC CCCGGCCCCT GCGCCATATG
    ACCAAAGTAA CATCAGGTTC AGAGTTGCCC ACCTCCAATG GAACCACCTT ATCCTCTAAC
    CTCACTGGGG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAA CGGTGGTCAG CCCCTCTACA GACAGTGGCC TGGAGTTCTC CTCTCAGACC
    ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAACCTGGCT CTCCAGGGTA TAGCACAGCT
    ACAGAGCCTG GTAGCAGTGA GCTAGGGGTT CCTGAGCAGC CTGACCCGCA GGAAGGAGTC
    CATGTGGAAA AGGCCCAGTC AGCATCTGTG GAATCCATCC CCGAAGTGTT AGAGGAGTGC
    ACGTCTCCTG CTGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC
    CGGGGTGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGTA CAGCTTTGGT TCCCTATGGT
    CTTCCCTGCA TCCGTGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACATGACCGC
    CACAATTCAG AAGTTATGAT CCACATGGGA CTGCATTTGC TGACAGTGGC TCTTGAATCT
    GCCCCTGTAG CCCAGTGCCA AACCCTTTTG GGCCTCATCA AAGATGAGAT GTGCCGCCAC
    TTACTCCAGC TACTCAGTGT AGAGCGACTC AACCTTTATG CTGCTTCCCT GCGGGTATGC
    TTCCTGCTTT TTGAGAGCAT GAGGGAACAT CTCAAGTTCC AATTGGAGAT GTACATCAAA
    AAGCTCATGG AGATCATCAC TGTGGAGAAC CCCAAAATGC CTTATGAGAT GAAAGAGATG
    GCACTGGAGG CCATTGTGCA GCTCTGGCAC ATCCCCAGTT TTGTCACTGA GCTCTATATC
    AACTATGATT GTGACTACTA CTGTTCAAAC CTCTTTGAAG ACCTCACCAA GCTGCTGTCC
    AAGAATGCCT TCCCTGTGTC TGGTCAGCTC TATACAACAC ACCTCCTGTC TCTTGATGCC
    CTGTTGACAG TGATTGACAG CACTGAGGCC CATTGCCAGG CCAAAGTCCT CAACAGTCTT
    ACCCAGCAAG AGAAGAAAGA AGCAGCCAGA TCTGGCTATG AGGCGGTGGA TGGTACCCGA
    GAATCCAGCA ATAGTGAGAG AGCAGCCAGC GATGGGAAAC CTGTAGGCAT AGCCGCAGAC
    ATCCCAGGTC TACATCTGCC AGGTGGAGGG CGGCTGCCAG CAGAACATGG GAAGCCAGGA
    TGCAGTGATC TGGAGGAAGC TGCTGACTCT GGGGCTGACA AAAAGTTTAC CCGGAAGCCA
    CCTCGATTTT CCTGTCTCCT GCCAGATCCA CGGGAACTGA TTGAAATTAA AAACAAAAAG
    AAGCTGCTAA TCACTGGCAC AGAGCAGTTC AACCAGAAAC CAAAGAAGGG AATCCAGTTT
    CTGCAGGAGA AAGGCCTCCT CACCATCCCA ATGGACAACA CAGAGGTAGC CCAGTGGCTC
    CGAGAGAACC CTCGGCTGGA CAAGAAAATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC
    ATTGACCTGT TGGAGAGCTT TGTGAGCACC TTCAGCTTTC AGGGTCTGCG GCTGGATGAA
    GCTCTTCGTC TCTACCTGGA AGCCTTTCGC TTACCTGGGG AAGCACCAGT CATCCAGAGG
    TTGCTGGAGG CATTCACAGA GCATTGGAGG AATTGTAATG GCTCCCCATT TGCCAATAGC
    GATGCCTGCT TTGCTCTGGC CTATGCTGTC ATCTTGCTTA ATACTGACCA GCACAACCAC
    AACGTTCGCA AACAGAATGC ACCCATGACT CTAGAGGAGT TTCGCAAAAA CCTAAAAGGT
    GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAGG ACATGTACCA TGCCATCAAG
    AATGAGGAAA TTGTGATGCC TGAAGAGCAG ACAGGCTTGG TTCGGGAGAA CTATGTGTGG
    AATGTGCTAC TTCATCGAGG TGCCACCCCA GAGGGCATAT TCCTTCGTGT GCCTGCTGGC
    AGCTATGATC TTGACCTCTT CACCATGACG TGGGGCCCCA CTATTGCTGC CCTTTCTTAT
    GTCTTTGACA AAAGCCTTGA GGAAACAATT ATCCAGAAAG CCATCTCAGG CTTCAGGAAG
    TGCGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATATCT
    CTGTGCAAAT TCACAGCTCT TAGCAGTGAG TCTATTGAGA ATCTGCCCAG TGTGTTTGGA
    AGCAACCCCA AAGCCCACAT TGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT
    GATATCCTGC GGGAGGGCTG GAAGAATATC ATGGAAGCCA TGCTGCAGCT CTTCCGAGCC
    CAACTGCTTC CCAAAGCTAT GGTGGAGGTA GAAGATTTTG TGGATCCCAA TGGCAAGATC
    TCTCTACAGA GGGAGGAGAC ACCATCAAAC CGAGGAGAGT CGACAGTGCT AAGCTTTGTG
    AGCTGGCTAA CACTAAGTGG TACTGAGCAG TCTAGTGTGC GGGGCCCATC CACTGAGAAC
    CAAGAGGCCA AAAGAGCGGC CTTGGACTGC ATCAAGCATT GTGACCCAGA AAAGATGATC
    ACAGAAAGCA AGTTCCTTCA GCTGGAGTCA CTGCAGGAGC TCATGAAGGC TCTGGTCTCA
    GTGACGCCAG ATGAAGAGAC ATATGATGAG GAGGATGCTG CTTTCTGCCT AGAGATGCTG
    CTGAGGATTG TGCTGGAGAA CAGGGACCGT GTGGGTTGTG TGTGGCAGAC TGTCCGAGAC
    CATCTATATC ACCTGTGTGT CCAGGCACAG GATTTCTGCT TCCTTGTGGA GCGGGCAGTG
    GTGGGGCTGC TCCGCTTGGC CATTCGACTA CTCCGAAGAG AAGAGATCAG TGGCCAGGTG
    CTGCTCTCCC TGCGTATTTT GCTACTAATG AAGCCCAGCG TGCTGTCCCG AGTCAGTCAC
    CAGGTAGCCT ATGGGCTCCA TGAACTTCTT AAGACCAACG CAGCCAACAT CCACTCAGGT
    GACGACTGGG CCACTCTCTT CACACTGCTG GAATGCATTG GCTCAGGTGT AAAGCCTCCA
    GCTGCCCTGC AGGCCACAGC CAGGGCTGAA GCACCTGATG CCGGGGCCCA ATCAGACAGC
    GAACTCCCAT CCTACCATCA GAATGATGTG AGCCTGGACC GAGGATACAC TTCTGACTCG
    GAGGTCTATG CTGACCATTG CAGGCCTGGC AAGATCCACC GATCAGCCAC TGATGCTGAT
    GTAGTCAACA GCGGCTGGTT AGTGGTGGGG AAGGATGACA TTGATAACTC CAAGCCAGGT
    CCTGGGCCCA GCCGGCTAGG CCCTTCACCC CTGGTCAATC AGTACAGCCT AACAGTGGGG
    CTGGACCTCG GGCCACACGA CACTAAGTCC CTGCTTAAAT GTGTAGAATC TCTGTCCTTC
    ATTGTGCGTG ATGCTGCCCA CATTACACCT GACAACTTTG AGCTCTGCGT GAAGACTCTC
    CGTATCTTTG TGGAGGCCAG TCTGAATGGC GGGTGCAAGT CCCAGGAGAA ACGTGGCAAG
    AGTCACAAAT ATGACAGCAA AGGAAACCGC TTCAAGAAGA AATCCAAGGA AGGTTCAGTG
    CTTCGGCGGC CTCGAACCTC TGGCCAACAT GCCACTCGGG GTGGGCATAG TGACGATGAT
    GAGGATGAAG GGGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGGTCAG TCAGGACTTG
    TTAGATCTGA TGCACACCCT GCACACGCGA GCAGCCTCTA TCTACAGCTC TTGGGCGGAG
    GAACAGCGCC ACCTAGAGAC AGGTGGCCGG AAGATAGAAG CGGATTCACG TACCCTCTGG
    GCCCACTGCT GGTGTCCCTT ACTGCAGGGC ATTGCCTGCT TGTGCTGTGA TGCCCGGCGC
    CAGGTGCGGA TGCAAGCACT GACTTATCTG CAACGAGCAC TACTGGTACA TGATCTGCAG
    AAGCTAGATG CCCTGGAGTG GGAGTCCTGT TTTAACAAGG TGCTGTTTCC TCTACTGACC
    AAACTCTTGG AGAACATCAG CCCTGCAGAT GTGGGTGGGA TGGAGGAGAC TCGAATGAGG
    GCTTCCACAC TGCTGTCTAA GGTCTTCCTG CAGCACCTGT CTCCACTGCT GTCACTCTCT
    ACCTTTGCGG CCCTCTGGCT GACCATCCTG GACTTCATGG ACAAGTATAT GCATGCAGGA
    TCCAGTGACT TACTGTCAGA GGCCATCCCT GAGTCTCTGA AGAACATGCT CCTGGTGATG
    GACACAGCAG AGATTTTCCA CAATGCAGAT GCCCGAGGAG GCAGCCCCTC AGCCCTCTGG
    GAGATCACCT GGGAGCGCAT TGACTGTTTT CTGCCCCACC TACGAGATGA GCTCTTCAAG
    CAGACTGTCA TCCAGGACCC CATGCCACTG GAGCCACATG CCCAAAAAAC TCTGGCCTCA
    GCCCACATGA CTCCTGCTGC TGGAGACACA AGGACACCTG GCCATCCACC TCCCCCAGAG
    ATGCCTTCTG AGCTGGGGGC CTGTGACTTC GAGAAGCCTG AGGGCCCTCG ACCTGCCAAC
    AGCAGCTCTC CTGGATCACC AGTGGCATCT AGCCCCAACA GACTGAGCCC TACTTCAGAT
    GGGCCTCCTC CTCTGGCTCA GCCTCCACTG ATCCTGCAGC CCTTGGCCTC CCCATTGCAG
    GTGGGCGTAC CACCCATGAC TCTACCAATC ATCCTCAACC CTGCTCTCAT TGAGGCCACT
    TCACCAGTGC CCCTCCTGGC CACACCCCGC CCCACAGACC CAATGCCCAC CTCCGAGGTC
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe43952
    Clone ID Related Accession (Same CDS sequence) XM_014108958.2 , XM_014108958.1
    Accession Version XM_014108958.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5574bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006610.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014108958.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
    AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA
    CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT
    CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC
    ATGAGTGATT CATCCAAGTG GAAGAAACAG AAGAGATCCC CCCGGCCCCT GCGCCATATG
    ACCAAAGTAA CATCAGGTTC AGAGTTGCCC ACCTCCAATG GAACCACCTT ATCCTCTAAC
    CTCACTGGGG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAA CGGTGGTCAG CCCCTCTACA GACAGTGGCC TGGAGTTCTC CTCTCAGACC
    ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAACCTGGCT CTCCAGGGTA TAGCACAGCT
    ACAGAGCCTG GTAGCAGTGA GCTAGGGGTT CCTGAGCAGC CTGACCCGCA GGAAGGAGTC
    CATGTGGAAA AGGCCCAGTC AGCATCTGTG GAATCCATCC CCGAAGTGTT AGAGGAGTGC
    ACGTCTCCTG CTGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC
    CGGGGTGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGTA CAGCTTTGGT TCCCTATGGT
    CTTCCCTGCA TCCGTGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACATGACCGC
    CACAATTCAG AAGTTATGAT CCACATGGGA CTGCATTTGC TGACAGTGGC TCTTGAATCT
    GCCCCTGTAG CCCAGTGCCA AACCCTTTTG GGCCTCATCA AAGATGAGAT GTGCCGCCAC
    TTACTCCAGC TACTCAGTGT AGAGCGACTC AACCTTTATG CTGCTTCCCT GCGGGTATGC
    TTCCTGCTTT TTGAGAGCAT GAGGGAACAT CTCAAGTTCC AATTGGAGAT GTACATCAAA
    AAGCTCATGG AGATCATCAC TGTGGAGAAC CCCAAAATGC CTTATGAGAT GAAAGAGATG
    GCACTGGAGG CCATTGTGCA GCTCTGGCAC ATCCCCAGTT TTGTCACTGA GCTCTATATC
    AACTATGATT GTGACTACTA CTGTTCAAAC CTCTTTGAAG ACCTCACCAA GCTGCTGTCC
    AAGAATGCCT TCCCTGTGTC TGGTCAGCTC TATACAACAC ACCTCCTGTC TCTTGATGCC
    CTGTTGACAG TGATTGACAG CACTGAGGCC CATTGCCAGG CCAAAGTCCT CAACAGTCTT
    ACCCAGCAAG AGAAGAAAGA AGCAGCCAGA TCTGGCTATG AGGCGGTGGA TGGTACCCGA
    GAATCCAGCA ATAGTGAGAG AGCAGCCAGC GATGGGAAAC CTGTAGGCAT AGCCGCAGAC
    ATCCCAGGTC TACATCTGCC AGGTGGAGGG CGGCTGCCAG CAGAACATGG GAAGCCAGGA
    TGCAGTGATC TGGAGGAAGC TGCTGACTCT GGGGCTGACA AAAAGTTTAC CCGGAAGCCA
    CCTCGATTTT CCTGTCTCCT GCCAGATCCA CGGGAACTGA TTGAAATTAA AAACAAAAAG
    AAGCTGCTAA TCACTGGCAC AGAGCAGTTC AACCAGAAAC CAAAGAAGGG AATCCAGTTT
    CTGCAGGAGA AAGGCCTCCT CACCATCCCA ATGGACAACA CAGAGGTAGC CCAGTGGCTC
    CGAGAGAACC CTCGGCTGGA CAAGAAAATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC
    ATTGACCTGT TGGAGAGCTT TGTGAGCACC TTCAGCTTTC AGGGTCTGCG GCTGGATGAA
    GCTCTTCGTC TCTACCTGGA AGCCTTTCGC TTACCTGGGG AAGCACCAGT CATCCAGAGG
    TTGCTGGAGG CATTCACAGA GCATTGGAGG AATTGTAATG GCTCCCCATT TGCCAATAGC
    GATGCCTGCT TTGCTCTGGC CTATGCTGTC ATCTTGCTTA ATACTGACCA GCACAACCAC
    AACGTTCGCA AACAGAATGC ACCCATGACT CTAGAGGAGT TTCGCAAAAA CCTAAAAGGT
    GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAGG ACATGTACCA TGCCATCAAG
    AATGAGGAAA TTGTGATGCC TGAAGAGCAG ACAGGCTTGG TTCGGGAGAA CTATGTGTGG
    AATGTGCTAC TTCATCGAGG TGCCACCCCA GAGGGCATAT TCCTTCGTGT GCCTGCTGGC
    AGCTATGATC TTGACCTCTT CACCATGACG TGGGGCCCCA CTATTGCTGC CCTTTCTTAT
    GTCTTTGACA AAAGCCTTGA GGAAACAATT ATCCAGAAAG CCATCTCAGG CTTCAGGAAG
    TGCGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATATCT
    CTGTGCAAAT TCACAGCTCT TAGCAGTGAG TCTATTGAGA ATCTGCCCAG TGTGTTTGGA
    AGCAACCCCA AAGCCCACAT TGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT
    GATATCCTGC GGGAGGGCTG GAAGAATATC ATGGAAGCCA TGCTGCAGCT CTTCCGAGCC
    CAACTGCTTC CCAAAGCTAT GGTGGAGGTA GAAGATTTTG TGGATCCCAA TGGCAAGATC
    TCTCTACAGA GGGAGGAGAC ACCATCAAAC CGAGGAGAGT CGACAGTGCT AAGCTTTGTG
    AGCTGGCTAA CACTAAGTGG TACTGAGCAG TCTAGTGTGC GGGGCCCATC CACTGAGAAC
    CAAGAGGCCA AAAGAGCGGC CTTGGACTGC ATCAAGCATT GTGACCCAGA AAAGATGATC
    ACAGAAAGCA AGTTCCTTCA GCTGGAGTCA CTGCAGGAGC TCATGAAGGC TCTGGTCTCA
    GTGACGCCAG ATGAAGAGAC ATATGATGAG GAGGATGCTG CTTTCTGCCT AGAGATGCTG
    CTGAGGATTG TGCTGGAGAA CAGGGACCGT GTGGGTTGTG TGTGGCAGAC TGTCCGAGAC
    CATCTATATC ACCTGTGTGT CCAGGCACAG GATTTCTGCT TCCTTGTGGA GCGGGCAGTG
    GTGGGGCTGC TCCGCTTGGC CATTCGACTA CTCCGAAGAG AAGAGATCAG TGGCCAGGTG
    CTGCTCTCCC TGCGTATTTT GCTACTAATG AAGCCCAGCG TGCTGTCCCG AGTCAGTCAC
    CAGGTAGCCT ATGGGCTCCA TGAACTTCTT AAGACCAACG CAGCCAACAT CCACTCAGGT
    GACGACTGGG CCACTCTCTT CACACTGCTG GAATGCATTG GCTCAGGTGT AAAGCCTCCA
    GCTGCCCTGC AGGCCACAGC CAGGGCTGAA GCACCTGATG CCGGGGCCCA ATCAGACAGC
    GAACTCCCAT CCTACCATCA GAATGATGTG AGCCTGGACC GAGGATACAC TTCTGACTCG
    GAGGTCTATG CTGACCATTG CAGGCCTGGC AAGATCCACC GATCAGCCAC TGATGCTGAT
    GTAGTCAACA GCGGCTGGTT AGTGGTGGGG AAGGATGACA TTGATAACTC CAAGCCAGGT
    CCTGGGCCCA GCCGGCTAGG CCCTTCACCC CTGGTCAATC AGTACAGCCT AACAGTGGGG
    CTGGACCTCG GGCCACACGA CACTAAGTCC CTGCTTAAAT GTGTAGAATC TCTGTCCTTC
    ATTGTGCGTG ATGCTGCCCA CATTACACCT GACAACTTTG AGCTCTGCGT GAAGACTCTC
    CGTATCTTTG TGGAGGCCAG TCTGAATGGC GGGTGCAAGT CCCAGGAGAA ACGTGGCAAG
    AGTCACAAAT ATGACAGCAA AGGAAACCGC TTCAAGAAGA AATCCAAGGA AGGTTCAGTG
    CTTCGGCGGC CTCGAACCTC TGGCCAACAT GCCACTCGGG GTGGGCATAG TGACGATGAT
    GAGGATGAAG GGGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGTTGTT AGATCTGATG
    CACACCCTGC ACACGCGAGC AGCCTCTATC TACAGCTCTT GGGCGGAGGA ACAGCGCCAC
    CTAGAGACAG GTGGCCGGAA GATAGAAGCG GATTCACGTA CCCTCTGGGC CCACTGCTGG
    TGTCCCTTAC TGCAGGGCAT TGCCTGCTTG TGCTGTGATG CCCGGCGCCA GGTGCGGATG
    CAAGCACTGA CTTATCTGCA ACGAGCACTA CTGGTACATG ATCTGCAGAA GCTAGATGCC
    CTGGAGTGGG AGTCCTGTTT TAACAAGGTG CTGTTTCCTC TACTGACCAA ACTCTTGGAG
    AACATCAGCC CTGCAGATGT GGGTGGGATG GAGGAGACTC GAATGAGGGC TTCCACACTG
    CTGTCTAAGG TCTTCCTGCA GCACCTGTCT CCACTGCTGT CACTCTCTAC CTTTGCGGCC
    CTCTGGCTGA CCATCCTGGA CTTCATGGAC AAGTATATGC ATGCAGGATC CAGTGACTTA
    CTGTCAGAGG CCATCCCTGA GTCTCTGAAG AACATGCTCC TGGTGATGGA CACAGCAGAG
    ATTTTCCACA ATGCAGATGC CCGAGGAGGC AGCCCCTCAG CCCTCTGGGA GATCACCTGG
    GAGCGCATTG ACTGTTTTCT GCCCCACCTA CGAGATGAGC TCTTCAAGCA GACTGTCATC
    CAGGACCCCA TGCCACTGGA GCCACATGCC CAAAAAACTC TGGCCTCAGC CCACATGACT
    CCTGCTGCTG GAGACACAAG GACACCTGGC CATCCACCTC CCCCAGAGAT GCCTTCTGAG
    CTGGGGGCCT GTGACTTCGA GAAGCCTGAG GGCCCTCGAC CTGCCAACAG CAGCTCTCCT
    GGATCACCAG TGGCATCTAG CCCCAACAGA CTGAGCCCTA CTTCAGATGG GCCTCCTCCT
    CTGGCTCAGC CTCCACTGAT CCTGCAGCCC TTGGCCTCCC CATTGCAGGT GGGCGTACCA
    CCCATGACTC TACCAATCAT CCTCAACCCT GCTCTCATTG AGGCCACTTC ACCAGTGCCC
    CTCCTGGCCA CACCCCGCCC CACAGACCCA ATGCCCACCT CCGAGGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013964433.1
    CDS269..5842
    Translation

    Target ORF information:

    RefSeq Version XM_014108958.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108958.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
    AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA
    CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT
    CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC
    ATGAGTGATT CATCCAAGTG GAAGAAACAG AAGAGATCCC CCCGGCCCCT GCGCCATATG
    ACCAAAGTAA CATCAGGTTC AGAGTTGCCC ACCTCCAATG GAACCACCTT ATCCTCTAAC
    CTCACTGGGG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAA CGGTGGTCAG CCCCTCTACA GACAGTGGCC TGGAGTTCTC CTCTCAGACC
    ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAACCTGGCT CTCCAGGGTA TAGCACAGCT
    ACAGAGCCTG GTAGCAGTGA GCTAGGGGTT CCTGAGCAGC CTGACCCGCA GGAAGGAGTC
    CATGTGGAAA AGGCCCAGTC AGCATCTGTG GAATCCATCC CCGAAGTGTT AGAGGAGTGC
    ACGTCTCCTG CTGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC
    CGGGGTGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGTA CAGCTTTGGT TCCCTATGGT
    CTTCCCTGCA TCCGTGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACATGACCGC
    CACAATTCAG AAGTTATGAT CCACATGGGA CTGCATTTGC TGACAGTGGC TCTTGAATCT
    GCCCCTGTAG CCCAGTGCCA AACCCTTTTG GGCCTCATCA AAGATGAGAT GTGCCGCCAC
    TTACTCCAGC TACTCAGTGT AGAGCGACTC AACCTTTATG CTGCTTCCCT GCGGGTATGC
    TTCCTGCTTT TTGAGAGCAT GAGGGAACAT CTCAAGTTCC AATTGGAGAT GTACATCAAA
    AAGCTCATGG AGATCATCAC TGTGGAGAAC CCCAAAATGC CTTATGAGAT GAAAGAGATG
    GCACTGGAGG CCATTGTGCA GCTCTGGCAC ATCCCCAGTT TTGTCACTGA GCTCTATATC
    AACTATGATT GTGACTACTA CTGTTCAAAC CTCTTTGAAG ACCTCACCAA GCTGCTGTCC
    AAGAATGCCT TCCCTGTGTC TGGTCAGCTC TATACAACAC ACCTCCTGTC TCTTGATGCC
    CTGTTGACAG TGATTGACAG CACTGAGGCC CATTGCCAGG CCAAAGTCCT CAACAGTCTT
    ACCCAGCAAG AGAAGAAAGA AGCAGCCAGA TCTGGCTATG AGGCGGTGGA TGGTACCCGA
    GAATCCAGCA ATAGTGAGAG AGCAGCCAGC GATGGGAAAC CTGTAGGCAT AGCCGCAGAC
    ATCCCAGGTC TACATCTGCC AGGTGGAGGG CGGCTGCCAG CAGAACATGG GAAGCCAGGA
    TGCAGTGATC TGGAGGAAGC TGCTGACTCT GGGGCTGACA AAAAGTTTAC CCGGAAGCCA
    CCTCGATTTT CCTGTCTCCT GCCAGATCCA CGGGAACTGA TTGAAATTAA AAACAAAAAG
    AAGCTGCTAA TCACTGGCAC AGAGCAGTTC AACCAGAAAC CAAAGAAGGG AATCCAGTTT
    CTGCAGGAGA AAGGCCTCCT CACCATCCCA ATGGACAACA CAGAGGTAGC CCAGTGGCTC
    CGAGAGAACC CTCGGCTGGA CAAGAAAATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC
    ATTGACCTGT TGGAGAGCTT TGTGAGCACC TTCAGCTTTC AGGGTCTGCG GCTGGATGAA
    GCTCTTCGTC TCTACCTGGA AGCCTTTCGC TTACCTGGGG AAGCACCAGT CATCCAGAGG
    TTGCTGGAGG CATTCACAGA GCATTGGAGG AATTGTAATG GCTCCCCATT TGCCAATAGC
    GATGCCTGCT TTGCTCTGGC CTATGCTGTC ATCTTGCTTA ATACTGACCA GCACAACCAC
    AACGTTCGCA AACAGAATGC ACCCATGACT CTAGAGGAGT TTCGCAAAAA CCTAAAAGGT
    GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAGG ACATGTACCA TGCCATCAAG
    AATGAGGAAA TTGTGATGCC TGAAGAGCAG ACAGGCTTGG TTCGGGAGAA CTATGTGTGG
    AATGTGCTAC TTCATCGAGG TGCCACCCCA GAGGGCATAT TCCTTCGTGT GCCTGCTGGC
    AGCTATGATC TTGACCTCTT CACCATGACG TGGGGCCCCA CTATTGCTGC CCTTTCTTAT
    GTCTTTGACA AAAGCCTTGA GGAAACAATT ATCCAGAAAG CCATCTCAGG CTTCAGGAAG
    TGCGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATATCT
    CTGTGCAAAT TCACAGCTCT TAGCAGTGAG TCTATTGAGA ATCTGCCCAG TGTGTTTGGA
    AGCAACCCCA AAGCCCACAT TGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT
    GATATCCTGC GGGAGGGCTG GAAGAATATC ATGGAAGCCA TGCTGCAGCT CTTCCGAGCC
    CAACTGCTTC CCAAAGCTAT GGTGGAGGTA GAAGATTTTG TGGATCCCAA TGGCAAGATC
    TCTCTACAGA GGGAGGAGAC ACCATCAAAC CGAGGAGAGT CGACAGTGCT AAGCTTTGTG
    AGCTGGCTAA CACTAAGTGG TACTGAGCAG TCTAGTGTGC GGGGCCCATC CACTGAGAAC
    CAAGAGGCCA AAAGAGCGGC CTTGGACTGC ATCAAGCATT GTGACCCAGA AAAGATGATC
    ACAGAAAGCA AGTTCCTTCA GCTGGAGTCA CTGCAGGAGC TCATGAAGGC TCTGGTCTCA
    GTGACGCCAG ATGAAGAGAC ATATGATGAG GAGGATGCTG CTTTCTGCCT AGAGATGCTG
    CTGAGGATTG TGCTGGAGAA CAGGGACCGT GTGGGTTGTG TGTGGCAGAC TGTCCGAGAC
    CATCTATATC ACCTGTGTGT CCAGGCACAG GATTTCTGCT TCCTTGTGGA GCGGGCAGTG
    GTGGGGCTGC TCCGCTTGGC CATTCGACTA CTCCGAAGAG AAGAGATCAG TGGCCAGGTG
    CTGCTCTCCC TGCGTATTTT GCTACTAATG AAGCCCAGCG TGCTGTCCCG AGTCAGTCAC
    CAGGTAGCCT ATGGGCTCCA TGAACTTCTT AAGACCAACG CAGCCAACAT CCACTCAGGT
    GACGACTGGG CCACTCTCTT CACACTGCTG GAATGCATTG GCTCAGGTGT AAAGCCTCCA
    GCTGCCCTGC AGGCCACAGC CAGGGCTGAA GCACCTGATG CCGGGGCCCA ATCAGACAGC
    GAACTCCCAT CCTACCATCA GAATGATGTG AGCCTGGACC GAGGATACAC TTCTGACTCG
    GAGGTCTATG CTGACCATTG CAGGCCTGGC AAGATCCACC GATCAGCCAC TGATGCTGAT
    GTAGTCAACA GCGGCTGGTT AGTGGTGGGG AAGGATGACA TTGATAACTC CAAGCCAGGT
    CCTGGGCCCA GCCGGCTAGG CCCTTCACCC CTGGTCAATC AGTACAGCCT AACAGTGGGG
    CTGGACCTCG GGCCACACGA CACTAAGTCC CTGCTTAAAT GTGTAGAATC TCTGTCCTTC
    ATTGTGCGTG ATGCTGCCCA CATTACACCT GACAACTTTG AGCTCTGCGT GAAGACTCTC
    CGTATCTTTG TGGAGGCCAG TCTGAATGGC GGGTGCAAGT CCCAGGAGAA ACGTGGCAAG
    AGTCACAAAT ATGACAGCAA AGGAAACCGC TTCAAGAAGA AATCCAAGGA AGGTTCAGTG
    CTTCGGCGGC CTCGAACCTC TGGCCAACAT GCCACTCGGG GTGGGCATAG TGACGATGAT
    GAGGATGAAG GGGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGTTGTT AGATCTGATG
    CACACCCTGC ACACGCGAGC AGCCTCTATC TACAGCTCTT GGGCGGAGGA ACAGCGCCAC
    CTAGAGACAG GTGGCCGGAA GATAGAAGCG GATTCACGTA CCCTCTGGGC CCACTGCTGG
    TGTCCCTTAC TGCAGGGCAT TGCCTGCTTG TGCTGTGATG CCCGGCGCCA GGTGCGGATG
    CAAGCACTGA CTTATCTGCA ACGAGCACTA CTGGTACATG ATCTGCAGAA GCTAGATGCC
    CTGGAGTGGG AGTCCTGTTT TAACAAGGTG CTGTTTCCTC TACTGACCAA ACTCTTGGAG
    AACATCAGCC CTGCAGATGT GGGTGGGATG GAGGAGACTC GAATGAGGGC TTCCACACTG
    CTGTCTAAGG TCTTCCTGCA GCACCTGTCT CCACTGCTGT CACTCTCTAC CTTTGCGGCC
    CTCTGGCTGA CCATCCTGGA CTTCATGGAC AAGTATATGC ATGCAGGATC CAGTGACTTA
    CTGTCAGAGG CCATCCCTGA GTCTCTGAAG AACATGCTCC TGGTGATGGA CACAGCAGAG
    ATTTTCCACA ATGCAGATGC CCGAGGAGGC AGCCCCTCAG CCCTCTGGGA GATCACCTGG
    GAGCGCATTG ACTGTTTTCT GCCCCACCTA CGAGATGAGC TCTTCAAGCA GACTGTCATC
    CAGGACCCCA TGCCACTGGA GCCACATGCC CAAAAAACTC TGGCCTCAGC CCACATGACT
    CCTGCTGCTG GAGACACAAG GACACCTGGC CATCCACCTC CCCCAGAGAT GCCTTCTGAG
    CTGGGGGCCT GTGACTTCGA GAAGCCTGAG GGCCCTCGAC CTGCCAACAG CAGCTCTCCT
    GGATCACCAG TGGCATCTAG CCCCAACAGA CTGAGCCCTA CTTCAGATGG GCCTCCTCCT
    CTGGCTCAGC CTCCACTGAT CCTGCAGCCC TTGGCCTCCC CATTGCAGGT GGGCGTACCA
    CCCATGACTC TACCAATCAT CCTCAACCCT GCTCTCATTG AGGCCACTTC ACCAGTGCCC
    CTCCTGGCCA CACCCCGCCC CACAGACCCA ATGCCCACCT CCGAGGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe43952
    Clone ID Related Accession (Same CDS sequence) XM_014108958.2 , XM_014108958.1
    Accession Version XM_014108958.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5574bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726109.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
    AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA
    CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT
    CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC
    ATGAGTGATT CATCCAAGTG GAAGAAACAG AAGAGATCCC CCCGGCCCCT GCGCCATATG
    ACCAAAGTAA CATCAGGTTC AGAGTTGCCC ACCTCCAATG GAACCACCTT ATCCTCTAAC
    CTCACTGGGG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAA CGGTGGTCAG CCCCTCTACA GACAGTGGCC TGGAGTTCTC CTCTCAGACC
    ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAACCTGGCT CTCCAGGGTA TAGCACAGCT
    ACAGAGCCTG GTAGCAGTGA GCTAGGGGTT CCTGAGCAGC CTGACCCGCA GGAAGGAGTC
    CATGTGGAAA AGGCCCAGTC AGCATCTGTG GAATCCATCC CCGAAGTGTT AGAGGAGTGC
    ACGTCTCCTG CTGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC
    CGGGGTGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGTA CAGCTTTGGT TCCCTATGGT
    CTTCCCTGCA TCCGTGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACATGACCGC
    CACAATTCAG AAGTTATGAT CCACATGGGA CTGCATTTGC TGACAGTGGC TCTTGAATCT
    GCCCCTGTAG CCCAGTGCCA AACCCTTTTG GGCCTCATCA AAGATGAGAT GTGCCGCCAC
    TTACTCCAGC TACTCAGTGT AGAGCGACTC AACCTTTATG CTGCTTCCCT GCGGGTATGC
    TTCCTGCTTT TTGAGAGCAT GAGGGAACAT CTCAAGTTCC AATTGGAGAT GTACATCAAA
    AAGCTCATGG AGATCATCAC TGTGGAGAAC CCCAAAATGC CTTATGAGAT GAAAGAGATG
    GCACTGGAGG CCATTGTGCA GCTCTGGCAC ATCCCCAGTT TTGTCACTGA GCTCTATATC
    AACTATGATT GTGACTACTA CTGTTCAAAC CTCTTTGAAG ACCTCACCAA GCTGCTGTCC
    AAGAATGCCT TCCCTGTGTC TGGTCAGCTC TATACAACAC ACCTCCTGTC TCTTGATGCC
    CTGTTGACAG TGATTGACAG CACTGAGGCC CATTGCCAGG CCAAAGTCCT CAACAGTCTT
    ACCCAGCAAG AGAAGAAAGA AGCAGCCAGA TCTGGCTATG AGGCGGTGGA TGGTACCCGA
    GAATCCAGCA ATAGTGAGAG AGCAGCCAGC GATGGGAAAC CTGTAGGCAT AGCCGCAGAC
    ATCCCAGGTC TACATCTGCC AGGTGGAGGG CGGCTGCCAG CAGAACATGG GAAGCCAGGA
    TGCAGTGATC TGGAGGAAGC TGCTGACTCT GGGGCTGACA AAAAGTTTAC CCGGAAGCCA
    CCTCGATTTT CCTGTCTCCT GCCAGATCCA CGGGAACTGA TTGAAATTAA AAACAAAAAG
    AAGCTGCTAA TCACTGGCAC AGAGCAGTTC AACCAGAAAC CAAAGAAGGG AATCCAGTTT
    CTGCAGGAGA AAGGCCTCCT CACCATCCCA ATGGACAACA CAGAGGTAGC CCAGTGGCTC
    CGAGAGAACC CTCGGCTGGA CAAGAAAATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC
    ATTGACCTGT TGGAGAGCTT TGTGAGCACC TTCAGCTTTC AGGGTCTGCG GCTGGATGAA
    GCTCTTCGTC TCTACCTGGA AGCCTTTCGC TTACCTGGGG AAGCACCAGT CATCCAGAGG
    TTGCTGGAGG CATTCACAGA GCATTGGAGG AATTGTAATG GCTCCCCATT TGCCAATAGC
    GATGCCTGCT TTGCTCTGGC CTATGCTGTC ATCTTGCTTA ATACTGACCA GCACAACCAC
    AACGTTCGCA AACAGAATGC ACCCATGACT CTAGAGGAGT TTCGCAAAAA CCTAAAAGGT
    GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAGG ACATGTACCA TGCCATCAAG
    AATGAGGAAA TTGTGATGCC TGAAGAGCAG ACAGGCTTGG TTCGGGAGAA CTATGTGTGG
    AATGTGCTAC TTCATCGAGG TGCCACCCCA GAGGGCATAT TCCTTCGTGT GCCTGCTGGC
    AGCTATGATC TTGACCTCTT CACCATGACG TGGGGCCCCA CTATTGCTGC CCTTTCTTAT
    GTCTTTGACA AAAGCCTTGA GGAAACAATT ATCCAGAAAG CCATCTCAGG CTTCAGGAAG
    TGCGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATATCT
    CTGTGCAAAT TCACAGCTCT TAGCAGTGAG TCTATTGAGA ATCTGCCCAG TGTGTTTGGA
    AGCAACCCCA AAGCCCACAT TGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT
    GATATCCTGC GGGAGGGCTG GAAGAATATC ATGGAAGCCA TGCTGCAGCT CTTCCGAGCC
    CAACTGCTTC CCAAAGCTAT GGTGGAGGTA GAAGATTTTG TGGATCCCAA TGGCAAGATC
    TCTCTACAGA GGGAGGAGAC ACCATCAAAC CGAGGAGAGT CGACAGTGCT AAGCTTTGTG
    AGCTGGCTAA CACTAAGTGG TACTGAGCAG TCTAGTGTGC GGGGCCCATC CACTGAGAAC
    CAAGAGGCCA AAAGAGCGGC CTTGGACTGC ATCAAGCATT GTGACCCAGA AAAGATGATC
    ACAGAAAGCA AGTTCCTTCA GCTGGAGTCA CTGCAGGAGC TCATGAAGGC TCTGGTCTCA
    GTGACGCCAG ATGAAGAGAC ATATGATGAG GAGGATGCTG CTTTCTGCCT AGAGATGCTG
    CTGAGGATTG TGCTGGAGAA CAGGGACCGT GTGGGTTGTG TGTGGCAGAC TGTCCGAGAC
    CATCTATATC ACCTGTGTGT CCAGGCACAG GATTTCTGCT TCCTTGTGGA GCGGGCAGTG
    GTGGGGCTGC TCCGCTTGGC CATTCGACTA CTCCGAAGAG AAGAGATCAG TGGCCAGGTG
    CTGCTCTCCC TGCGTATTTT GCTACTAATG AAGCCCAGCG TGCTGTCCCG AGTCAGTCAC
    CAGGTAGCCT ATGGGCTCCA TGAACTTCTT AAGACCAACG CAGCCAACAT CCACTCAGGT
    GACGACTGGG CCACTCTCTT CACACTGCTG GAATGCATTG GCTCAGGTGT AAAGCCTCCA
    GCTGCCCTGC AGGCCACAGC CAGGGCTGAA GCACCTGATG CCGGGGCCCA ATCAGACAGC
    GAACTCCCAT CCTACCATCA GAATGATGTG AGCCTGGACC GAGGATACAC TTCTGACTCG
    GAGGTCTATG CTGACCATTG CAGGCCTGGC AAGATCCACC GATCAGCCAC TGATGCTGAT
    GTAGTCAACA GCGGCTGGTT AGTGGTGGGG AAGGATGACA TTGATAACTC CAAGCCAGGT
    CCTGGGCCCA GCCGGCTAGG CCCTTCACCC CTGGTCAATC AGTACAGCCT AACAGTGGGG
    CTGGACCTCG GGCCACACGA CACTAAGTCC CTGCTTAAAT GTGTAGAATC TCTGTCCTTC
    ATTGTGCGTG ATGCTGCCCA CATTACACCT GACAACTTTG AGCTCTGCGT GAAGACTCTC
    CGTATCTTTG TGGAGGCCAG TCTGAATGGC GGGTGCAAGT CCCAGGAGAA ACGTGGCAAG
    AGTCACAAAT ATGACAGCAA AGGAAACCGC TTCAAGAAGA AATCCAAGGA AGGTTCAGTG
    CTTCGGCGGC CTCGAACCTC TGGCCAACAT GCCACTCGGG GTGGGCATAG TGACGATGAT
    GAGGATGAAG GGGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGTTGTT AGATCTGATG
    CACACCCTGC ACACGCGAGC AGCCTCTATC TACAGCTCTT GGGCGGAGGA ACAGCGCCAC
    CTAGAGACAG GTGGCCGGAA GATAGAAGCG GATTCACGTA CCCTCTGGGC CCACTGCTGG
    TGTCCCTTAC TGCAGGGCAT TGCCTGCTTG TGCTGTGATG CCCGGCGCCA GGTGCGGATG
    CAAGCACTGA CTTATCTGCA ACGAGCACTA CTGGTACATG ATCTGCAGAA GCTAGATGCC
    CTGGAGTGGG AGTCCTGTTT TAACAAGGTG CTGTTTCCTC TACTGACCAA ACTCTTGGAG
    AACATCAGCC CTGCAGATGT GGGTGGGATG GAGGAGACTC GAATGAGGGC TTCCACACTG
    CTGTCTAAGG TCTTCCTGCA GCACCTGTCT CCACTGCTGT CACTCTCTAC CTTTGCGGCC
    CTCTGGCTGA CCATCCTGGA CTTCATGGAC AAGTATATGC ATGCAGGATC CAGTGACTTA
    CTGTCAGAGG CCATCCCTGA GTCTCTGAAG AACATGCTCC TGGTGATGGA CACAGCAGAG
    ATTTTCCACA ATGCAGATGC CCGAGGAGGC AGCCCCTCAG CCCTCTGGGA GATCACCTGG
    GAGCGCATTG ACTGTTTTCT GCCCCACCTA CGAGATGAGC TCTTCAAGCA GACTGTCATC
    CAGGACCCCA TGCCACTGGA GCCACATGCC CAAAAAACTC TGGCCTCAGC CCACATGACT
    CCTGCTGCTG GAGACACAAG GACACCTGGC CATCCACCTC CCCCAGAGAT GCCTTCTGAG
    CTGGGGGCCT GTGACTTCGA GAAGCCTGAG GGCCCTCGAC CTGCCAACAG CAGCTCTCCT
    GGATCACCAG TGGCATCTAG CCCCAACAGA CTGAGCCCTA CTTCAGATGG GCCTCCTCCT
    CTGGCTCAGC CTCCACTGAT CCTGCAGCCC TTGGCCTCCC CATTGCAGGT GGGCGTACCA
    CCCATGACTC TACCAATCAT CCTCAACCCT GCTCTCATTG AGGCCACTTC ACCAGTGCCC
    CTCCTGGCCA CACCCCGCCC CACAGACCCA ATGCCCACCT CCGAGGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013964433.1
    CDS251..5824
    Translation

    Target ORF information:

    RefSeq Version XM_014108958.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108958.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
    AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA
    CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT
    CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC
    ATGAGTGATT CATCCAAGTG GAAGAAACAG AAGAGATCCC CCCGGCCCCT GCGCCATATG
    ACCAAAGTAA CATCAGGTTC AGAGTTGCCC ACCTCCAATG GAACCACCTT ATCCTCTAAC
    CTCACTGGGG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAA CGGTGGTCAG CCCCTCTACA GACAGTGGCC TGGAGTTCTC CTCTCAGACC
    ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAACCTGGCT CTCCAGGGTA TAGCACAGCT
    ACAGAGCCTG GTAGCAGTGA GCTAGGGGTT CCTGAGCAGC CTGACCCGCA GGAAGGAGTC
    CATGTGGAAA AGGCCCAGTC AGCATCTGTG GAATCCATCC CCGAAGTGTT AGAGGAGTGC
    ACGTCTCCTG CTGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC
    CGGGGTGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGTA CAGCTTTGGT TCCCTATGGT
    CTTCCCTGCA TCCGTGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACATGACCGC
    CACAATTCAG AAGTTATGAT CCACATGGGA CTGCATTTGC TGACAGTGGC TCTTGAATCT
    GCCCCTGTAG CCCAGTGCCA AACCCTTTTG GGCCTCATCA AAGATGAGAT GTGCCGCCAC
    TTACTCCAGC TACTCAGTGT AGAGCGACTC AACCTTTATG CTGCTTCCCT GCGGGTATGC
    TTCCTGCTTT TTGAGAGCAT GAGGGAACAT CTCAAGTTCC AATTGGAGAT GTACATCAAA
    AAGCTCATGG AGATCATCAC TGTGGAGAAC CCCAAAATGC CTTATGAGAT GAAAGAGATG
    GCACTGGAGG CCATTGTGCA GCTCTGGCAC ATCCCCAGTT TTGTCACTGA GCTCTATATC
    AACTATGATT GTGACTACTA CTGTTCAAAC CTCTTTGAAG ACCTCACCAA GCTGCTGTCC
    AAGAATGCCT TCCCTGTGTC TGGTCAGCTC TATACAACAC ACCTCCTGTC TCTTGATGCC
    CTGTTGACAG TGATTGACAG CACTGAGGCC CATTGCCAGG CCAAAGTCCT CAACAGTCTT
    ACCCAGCAAG AGAAGAAAGA AGCAGCCAGA TCTGGCTATG AGGCGGTGGA TGGTACCCGA
    GAATCCAGCA ATAGTGAGAG AGCAGCCAGC GATGGGAAAC CTGTAGGCAT AGCCGCAGAC
    ATCCCAGGTC TACATCTGCC AGGTGGAGGG CGGCTGCCAG CAGAACATGG GAAGCCAGGA
    TGCAGTGATC TGGAGGAAGC TGCTGACTCT GGGGCTGACA AAAAGTTTAC CCGGAAGCCA
    CCTCGATTTT CCTGTCTCCT GCCAGATCCA CGGGAACTGA TTGAAATTAA AAACAAAAAG
    AAGCTGCTAA TCACTGGCAC AGAGCAGTTC AACCAGAAAC CAAAGAAGGG AATCCAGTTT
    CTGCAGGAGA AAGGCCTCCT CACCATCCCA ATGGACAACA CAGAGGTAGC CCAGTGGCTC
    CGAGAGAACC CTCGGCTGGA CAAGAAAATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC
    ATTGACCTGT TGGAGAGCTT TGTGAGCACC TTCAGCTTTC AGGGTCTGCG GCTGGATGAA
    GCTCTTCGTC TCTACCTGGA AGCCTTTCGC TTACCTGGGG AAGCACCAGT CATCCAGAGG
    TTGCTGGAGG CATTCACAGA GCATTGGAGG AATTGTAATG GCTCCCCATT TGCCAATAGC
    GATGCCTGCT TTGCTCTGGC CTATGCTGTC ATCTTGCTTA ATACTGACCA GCACAACCAC
    AACGTTCGCA AACAGAATGC ACCCATGACT CTAGAGGAGT TTCGCAAAAA CCTAAAAGGT
    GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAGG ACATGTACCA TGCCATCAAG
    AATGAGGAAA TTGTGATGCC TGAAGAGCAG ACAGGCTTGG TTCGGGAGAA CTATGTGTGG
    AATGTGCTAC TTCATCGAGG TGCCACCCCA GAGGGCATAT TCCTTCGTGT GCCTGCTGGC
    AGCTATGATC TTGACCTCTT CACCATGACG TGGGGCCCCA CTATTGCTGC CCTTTCTTAT
    GTCTTTGACA AAAGCCTTGA GGAAACAATT ATCCAGAAAG CCATCTCAGG CTTCAGGAAG
    TGCGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATATCT
    CTGTGCAAAT TCACAGCTCT TAGCAGTGAG TCTATTGAGA ATCTGCCCAG TGTGTTTGGA
    AGCAACCCCA AAGCCCACAT TGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT
    GATATCCTGC GGGAGGGCTG GAAGAATATC ATGGAAGCCA TGCTGCAGCT CTTCCGAGCC
    CAACTGCTTC CCAAAGCTAT GGTGGAGGTA GAAGATTTTG TGGATCCCAA TGGCAAGATC
    TCTCTACAGA GGGAGGAGAC ACCATCAAAC CGAGGAGAGT CGACAGTGCT AAGCTTTGTG
    AGCTGGCTAA CACTAAGTGG TACTGAGCAG TCTAGTGTGC GGGGCCCATC CACTGAGAAC
    CAAGAGGCCA AAAGAGCGGC CTTGGACTGC ATCAAGCATT GTGACCCAGA AAAGATGATC
    ACAGAAAGCA AGTTCCTTCA GCTGGAGTCA CTGCAGGAGC TCATGAAGGC TCTGGTCTCA
    GTGACGCCAG ATGAAGAGAC ATATGATGAG GAGGATGCTG CTTTCTGCCT AGAGATGCTG
    CTGAGGATTG TGCTGGAGAA CAGGGACCGT GTGGGTTGTG TGTGGCAGAC TGTCCGAGAC
    CATCTATATC ACCTGTGTGT CCAGGCACAG GATTTCTGCT TCCTTGTGGA GCGGGCAGTG
    GTGGGGCTGC TCCGCTTGGC CATTCGACTA CTCCGAAGAG AAGAGATCAG TGGCCAGGTG
    CTGCTCTCCC TGCGTATTTT GCTACTAATG AAGCCCAGCG TGCTGTCCCG AGTCAGTCAC
    CAGGTAGCCT ATGGGCTCCA TGAACTTCTT AAGACCAACG CAGCCAACAT CCACTCAGGT
    GACGACTGGG CCACTCTCTT CACACTGCTG GAATGCATTG GCTCAGGTGT AAAGCCTCCA
    GCTGCCCTGC AGGCCACAGC CAGGGCTGAA GCACCTGATG CCGGGGCCCA ATCAGACAGC
    GAACTCCCAT CCTACCATCA GAATGATGTG AGCCTGGACC GAGGATACAC TTCTGACTCG
    GAGGTCTATG CTGACCATTG CAGGCCTGGC AAGATCCACC GATCAGCCAC TGATGCTGAT
    GTAGTCAACA GCGGCTGGTT AGTGGTGGGG AAGGATGACA TTGATAACTC CAAGCCAGGT
    CCTGGGCCCA GCCGGCTAGG CCCTTCACCC CTGGTCAATC AGTACAGCCT AACAGTGGGG
    CTGGACCTCG GGCCACACGA CACTAAGTCC CTGCTTAAAT GTGTAGAATC TCTGTCCTTC
    ATTGTGCGTG ATGCTGCCCA CATTACACCT GACAACTTTG AGCTCTGCGT GAAGACTCTC
    CGTATCTTTG TGGAGGCCAG TCTGAATGGC GGGTGCAAGT CCCAGGAGAA ACGTGGCAAG
    AGTCACAAAT ATGACAGCAA AGGAAACCGC TTCAAGAAGA AATCCAAGGA AGGTTCAGTG
    CTTCGGCGGC CTCGAACCTC TGGCCAACAT GCCACTCGGG GTGGGCATAG TGACGATGAT
    GAGGATGAAG GGGTGCCTGC CAGCTACCAT ACGGTGTCTT TACAGTTGTT AGATCTGATG
    CACACCCTGC ACACGCGAGC AGCCTCTATC TACAGCTCTT GGGCGGAGGA ACAGCGCCAC
    CTAGAGACAG GTGGCCGGAA GATAGAAGCG GATTCACGTA CCCTCTGGGC CCACTGCTGG
    TGTCCCTTAC TGCAGGGCAT TGCCTGCTTG TGCTGTGATG CCCGGCGCCA GGTGCGGATG
    CAAGCACTGA CTTATCTGCA ACGAGCACTA CTGGTACATG ATCTGCAGAA GCTAGATGCC
    CTGGAGTGGG AGTCCTGTTT TAACAAGGTG CTGTTTCCTC TACTGACCAA ACTCTTGGAG
    AACATCAGCC CTGCAGATGT GGGTGGGATG GAGGAGACTC GAATGAGGGC TTCCACACTG
    CTGTCTAAGG TCTTCCTGCA GCACCTGTCT CCACTGCTGT CACTCTCTAC CTTTGCGGCC
    CTCTGGCTGA CCATCCTGGA CTTCATGGAC AAGTATATGC ATGCAGGATC CAGTGACTTA
    CTGTCAGAGG CCATCCCTGA GTCTCTGAAG AACATGCTCC TGGTGATGGA CACAGCAGAG
    ATTTTCCACA ATGCAGATGC CCGAGGAGGC AGCCCCTCAG CCCTCTGGGA GATCACCTGG
    GAGCGCATTG ACTGTTTTCT GCCCCACCTA CGAGATGAGC TCTTCAAGCA GACTGTCATC
    CAGGACCCCA TGCCACTGGA GCCACATGCC CAAAAAACTC TGGCCTCAGC CCACATGACT
    CCTGCTGCTG GAGACACAAG GACACCTGGC CATCCACCTC CCCCAGAGAT GCCTTCTGAG
    CTGGGGGCCT GTGACTTCGA GAAGCCTGAG GGCCCTCGAC CTGCCAACAG CAGCTCTCCT
    GGATCACCAG TGGCATCTAG CCCCAACAGA CTGAGCCCTA CTTCAGATGG GCCTCCTCCT
    CTGGCTCAGC CTCCACTGAT CCTGCAGCCC TTGGCCTCCC CATTGCAGGT GGGCGTACCA
    CCCATGACTC TACCAATCAT CCTCAACCCT GCTCTCATTG AGGCCACTTC ACCAGTGCCC
    CTCCTGGCCA CACCCCGCCC CACAGACCCA ATGCCCACCT CCGAGGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27947
    Clone ID Related Accession (Same CDS sequence) XM_005637657.3 , XM_005637657.2
    Accession Version XM_005637657.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5685bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006610.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005637657.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
    AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA
    CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT
    CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGA TTTCTCCATG TCTCAACAAA
    CTGGAGCTAA GTAGTGGGGA GCAGACCAAA GCCCTGAACC AGTTAGAGAG GGTACTACTC
    TTTAAGAACC TCAAGCTGAA AATGAGAGCA GGAGGCATGA GTGATTCATC CAAGTGGAAG
    AAACAGAAGA GATCCCCCCG GCCCCTGCGC CATATGACCA AAGTAACATC AGGTTCAGAG
    TTGCCCACCT CCAATGGAAC CACCTTATCC TCTAACCTCA CTGGGGGCAT GCCCTTCATT
    GATGTGCCCA CTCCCATCTC CTCTGCAAGT TCAGAAGCTG CCTCAACGGT GGTCAGCCCC
    TCTACAGACA GTGGCCTGGA GTTCTCCTCT CAGACCACCT CCAAGGAGGA CCTCACTGAC
    CTGGAGCAAC CTGGCTCTCC AGGGTATAGC ACAGCTACAG AGCCTGGTAG CAGTGAGCTA
    GGGGTTCCTG AGCAGCCTGA CCCGCAGCAG GAAGGAGTCC ATGTGGAAAA GGCCCAGTCA
    GCATCTGTGG AATCCATCCC CGAAGTGTTA GAGGAGTGCA CGTCTCCTGC TGACCACTCT
    GACTCTGCCT CTGTCCATGA CATGGATTAC GTCAATCCCC GGGGTGTGCG CTTTACACAG
    TCCTCCCAGA AAGAAGGTAC AGCTTTGGTT CCCTATGGTC TTCCCTGCAT CCGTGAGCTC
    TTCCGCTTCC TCATCTCCCT CACCAATCCA CATGACCGCC ACAATTCAGA AGTTATGATC
    CACATGGGAC TGCATTTGCT GACAGTGGCT CTTGAATCTG CCCCTGTAGC CCAGTGCCAA
    ACCCTTTTGG GCCTCATCAA AGATGAGATG TGCCGCCACT TACTCCAGCT ACTCAGTGTA
    GAGCGACTCA ACCTTTATGC TGCTTCCCTG CGGGTATGCT TCCTGCTTTT TGAGAGCATG
    AGGGAACATC TCAAGTTCCA ATTGGAGATG TACATCAAAA AGCTCATGGA GATCATCACT
    GTGGAGAACC CCAAAATGCC TTATGAGATG AAAGAGATGG CACTGGAGGC CATTGTGCAG
    CTCTGGCACA TCCCCAGTTT TGTCACTGAG CTCTATATCA ACTATGATTG TGACTACTAC
    TGTTCAAACC TCTTTGAAGA CCTCACCAAG CTGCTGTCCA AGAATGCCTT CCCTGTGTCT
    GGTCAGCTCT ATACAACACA CCTCCTGTCT CTTGATGCCC TGTTGACAGT GATTGACAGC
    ACTGAGGCCC ATTGCCAGGC CAAAGTCCTC AACAGTCTTA CCCAGCAAGA GAAGAAAGAA
    GCAGCCAGAT CTGGCTATGA GGCGGTGGAT GGTACCCGAG AATCCAGCAA TAGTGAGAGA
    GCAGCCAGCG ATGGGAAACC TGTAGGCATA GCCGCAGACA TCCCAGGTCT ACATCTGCCA
    GGTGGAGGGC GGCTGCCAGC AGAACATGGG AAGCCAGGAT GCAGTGATCT GGAGGAAGCT
    GCTGACTCTG GGGCTGACAA AAAGTTTACC CGGAAGCCAC CTCGATTTTC CTGTCTCCTG
    CCAGATCCAC GGGAACTGAT TGAAATTAAA AACAAAAAGA AGCTGCTAAT CACTGGCACA
    GAGCAGTTCA ACCAGAAACC AAAGAAGGGA ATCCAGTTTC TGCAGGAGAA AGGCCTCCTC
    ACCATCCCAA TGGACAACAC AGAGGTAGCC CAGTGGCTCC GAGAGAACCC TCGGCTGGAC
    AAGAAAATGA TTGGAGAGTT TGTGAGTGAC CGCAAAAACA TTGACCTGTT GGAGAGCTTT
    GTGAGCACCT TCAGCTTTCA GGGTCTGCGG CTGGATGAAG CTCTTCGTCT CTACCTGGAA
    GCCTTTCGCT TACCTGGGGA AGCACCAGTC ATCCAGAGGT TGCTGGAGGC ATTCACAGAG
    CATTGGAGGA ATTGTAATGG CTCCCCATTT GCCAATAGCG ATGCCTGCTT TGCTCTGGCC
    TATGCTGTCA TCTTGCTTAA TACTGACCAG CACAACCACA ACGTTCGCAA ACAGAATGCA
    CCCATGACTC TAGAGGAGTT TCGCAAAAAC CTAAAAGGTG TGAATGGAGG CAAGGACTTT
    GAGCAAGACA TCCTGGAGGA CATGTACCAT GCCATCAAGA ATGAGGAAAT TGTGATGCCT
    GAAGAGCAGA CAGGCTTGGT TCGGGAGAAC TATGTGTGGA ATGTGCTACT TCATCGAGGT
    GCCACCCCAG AGGGCATATT CCTTCGTGTG CCTGCTGGCA GCTATGATCT TGACCTCTTC
    ACCATGACGT GGGGCCCCAC TATTGCTGCC CTTTCTTATG TCTTTGACAA AAGCCTTGAG
    GAAACAATTA TCCAGAAAGC CATCTCAGGC TTCAGGAAGT GCGCCATGAT CTCCGCCCAC
    TATGGCCTCA GCGATGTGTT TGACAATCTC ATCATATCTC TGTGCAAATT CACAGCTCTT
    AGCAGTGAGT CTATTGAGAA TCTGCCCAGT GTGTTTGGAA GCAACCCCAA AGCCCACATT
    GCAGCCAAGA CAGTATTCCA TTTGGCCCAT CGTCATGGTG ATATCCTGCG GGAGGGCTGG
    AAGAATATCA TGGAAGCCAT GCTGCAGCTC TTCCGAGCCC AACTGCTTCC CAAAGCTATG
    GTGGAGGTAG AAGATTTTGT GGATCCCAAT GGCAAGATCT CTCTACAGAG GGAGGAGACA
    CCATCAAACC GAGGAGAGTC GACAGTGCTA AGCTTTGTGA GCTGGCTAAC ACTAAGTGGT
    ACTGAGCAGT CTAGTGTGCG GGGCCCATCC ACTGAGAACC AAGAGGCCAA AAGAGCGGCC
    TTGGACTGCA TCAAGCATTG TGACCCAGAA AAGATGATCA CAGAAAGCAA GTTCCTTCAG
    CTGGAGTCAC TGCAGGAGCT CATGAAGGCT CTGGTCTCAG TGACGCCAGA TGAAGAGACA
    TATGATGAGG AGGATGCTGC TTTCTGCCTA GAGATGCTGC TGAGGATTGT GCTGGAGAAC
    AGGGACCGTG TGGGTTGTGT GTGGCAGACT GTCCGAGACC ATCTATATCA CCTGTGTGTC
    CAGGCACAGG ATTTCTGCTT CCTTGTGGAG CGGGCAGTGG TGGGGCTGCT CCGCTTGGCC
    ATTCGACTAC TCCGAAGAGA AGAGATCAGT GGCCAGGTGC TGCTCTCCCT GCGTATTTTG
    CTACTAATGA AGCCCAGCGT GCTGTCCCGA GTCAGTCACC AGGTAGCCTA TGGGCTCCAT
    GAACTTCTTA AGACCAACGC AGCCAACATC CACTCAGGTG ACGACTGGGC CACTCTCTTC
    ACACTGCTGG AATGCATTGG CTCAGGTGTA AAGCCTCCAG CTGCCCTGCA GGCCACAGCC
    AGGGCTGAAG CACCTGATGC CGGGGCCCAA TCAGACAGCG AACTCCCATC CTACCATCAG
    AATGATGTGA GCCTGGACCG AGGATACACT TCTGACTCGG AGGTCTATGC TGACCATTGC
    AGGCCTGGCA AGATCCACCG ATCAGCCACT GATGCTGATG TAGTCAACAG CGGCTGGTTA
    GTGGTGGGGA AGGATGACAT TGATAACTCC AAGCCAGGTC CTGGGCCCAG CCGGCTAGGC
    CCTTCACCCC TGGTCAATCA GTACAGCCTA ACAGTGGGGC TGGACCTCGG GCCACACGAC
    ACTAAGTCCC TGCTTAAATG TGTAGAATCT CTGTCCTTCA TTGTGCGTGA TGCTGCCCAC
    ATTACACCTG ACAACTTTGA GCTCTGCGTG AAGACTCTCC GTATCTTTGT GGAGGCCAGT
    CTGAATGGCG GGTGCAAGTC CCAGGAGAAA CGTGGCAAGA GTCACAAATA TGACAGCAAA
    GGAAACCGCT TCAAGAAGAA ATCCAAGGAA GGTTCAGTGC TTCGGCGGCC TCGAACCTCT
    GGCCAACATG CCACTCGGGG TGGGCATAGT GACGATGATG AGGATGAAGG GGTGCCTGCC
    AGCTACCATA CGGTGTCTTT ACAGGTCAGT CAGGACTTGT TAGATCTGAT GCACACCCTG
    CACACGCGAG CAGCCTCTAT CTACAGCTCT TGGGCGGAGG AACAGCGCCA CCTAGAGACA
    GGTGGCCGGA AGATAGAAGC GGATTCACGT ACCCTCTGGG CCCACTGCTG GTGTCCCTTA
    CTGCAGGGCA TTGCCTGCTT GTGCTGTGAT GCCCGGCGCC AGGTGCGGAT GCAAGCACTG
    ACTTATCTGC AACGAGCACT ACTGGTACAT GATCTGCAGA AGCTAGATGC CCTGGAGTGG
    GAGTCCTGTT TTAACAAGGT GCTGTTTCCT CTACTGACCA AACTCTTGGA GAACATCAGC
    CCTGCAGATG TGGGTGGGAT GGAGGAGACT CGAATGAGGG CTTCCACACT GCTGTCTAAG
    GTCTTCCTGC AGCACCTGTC TCCACTGCTG TCACTCTCTA CCTTTGCGGC CCTCTGGCTG
    ACCATCCTGG ACTTCATGGA CAAGTATATG CATGCAGGAT CCAGTGACTT ACTGTCAGAG
    GCCATCCCTG AGTCTCTGAA GAACATGCTC CTGGTGATGG ACACAGCAGA GATTTTCCAC
    AATGCAGATG CCCGAGGAGG CAGCCCCTCA GCCCTCTGGG AGATCACCTG GGAGCGCATT
    GACTGTTTTC TGCCCCACCT ACGAGATGAG CTCTTCAAGC AGACTGTCAT CCAGGACCCC
    ATGCCACTGG AGCCACATGC CCAAAAAACT CTGGCCTCAG CCCACATGAC TCCTGCTGCT
    GGAGACACAA GGACACCTGG CCATCCACCT CCCCCAGAGA TGCCTTCTGA GCTGGGGGCC
    TGTGACTTCG AGAAGCCTGA GGGCCCTCGA CCTGCCAACA GCAGCTCTCC TGGATCACCA
    GTGGCATCTA GCCCCAACAG ACTGAGCCCT ACTTCAGATG GGCCTCCTCC TCTGGCTCAG
    CCTCCACTGA TCCTGCAGCC CTTGGCCTCC CCATTGCAGG TGGGCGTACC ACCCATGACT
    CTACCAATCA TCCTCAACCC TGCTCTCATT GAGGCCACTT CACCAGTGCC CCTCCTGGCC
    ACACCCCGCC CCACAGACCC AATGCCCACC TCCGAGGTCA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005637714.1
    CDS269..5953
    Translation

    Target ORF information:

    RefSeq Version XM_005637657.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005637657.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
    AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA
    CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT
    CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGA TTTCTCCATG TCTCAACAAA
    CTGGAGCTAA GTAGTGGGGA GCAGACCAAA GCCCTGAACC AGTTAGAGAG GGTACTACTC
    TTTAAGAACC TCAAGCTGAA AATGAGAGCA GGAGGCATGA GTGATTCATC CAAGTGGAAG
    AAACAGAAGA GATCCCCCCG GCCCCTGCGC CATATGACCA AAGTAACATC AGGTTCAGAG
    TTGCCCACCT CCAATGGAAC CACCTTATCC TCTAACCTCA CTGGGGGCAT GCCCTTCATT
    GATGTGCCCA CTCCCATCTC CTCTGCAAGT TCAGAAGCTG CCTCAACGGT GGTCAGCCCC
    TCTACAGACA GTGGCCTGGA GTTCTCCTCT CAGACCACCT CCAAGGAGGA CCTCACTGAC
    CTGGAGCAAC CTGGCTCTCC AGGGTATAGC ACAGCTACAG AGCCTGGTAG CAGTGAGCTA
    GGGGTTCCTG AGCAGCCTGA CCCGCAGCAG GAAGGAGTCC ATGTGGAAAA GGCCCAGTCA
    GCATCTGTGG AATCCATCCC CGAAGTGTTA GAGGAGTGCA CGTCTCCTGC TGACCACTCT
    GACTCTGCCT CTGTCCATGA CATGGATTAC GTCAATCCCC GGGGTGTGCG CTTTACACAG
    TCCTCCCAGA AAGAAGGTAC AGCTTTGGTT CCCTATGGTC TTCCCTGCAT CCGTGAGCTC
    TTCCGCTTCC TCATCTCCCT CACCAATCCA CATGACCGCC ACAATTCAGA AGTTATGATC
    CACATGGGAC TGCATTTGCT GACAGTGGCT CTTGAATCTG CCCCTGTAGC CCAGTGCCAA
    ACCCTTTTGG GCCTCATCAA AGATGAGATG TGCCGCCACT TACTCCAGCT ACTCAGTGTA
    GAGCGACTCA ACCTTTATGC TGCTTCCCTG CGGGTATGCT TCCTGCTTTT TGAGAGCATG
    AGGGAACATC TCAAGTTCCA ATTGGAGATG TACATCAAAA AGCTCATGGA GATCATCACT
    GTGGAGAACC CCAAAATGCC TTATGAGATG AAAGAGATGG CACTGGAGGC CATTGTGCAG
    CTCTGGCACA TCCCCAGTTT TGTCACTGAG CTCTATATCA ACTATGATTG TGACTACTAC
    TGTTCAAACC TCTTTGAAGA CCTCACCAAG CTGCTGTCCA AGAATGCCTT CCCTGTGTCT
    GGTCAGCTCT ATACAACACA CCTCCTGTCT CTTGATGCCC TGTTGACAGT GATTGACAGC
    ACTGAGGCCC ATTGCCAGGC CAAAGTCCTC AACAGTCTTA CCCAGCAAGA GAAGAAAGAA
    GCAGCCAGAT CTGGCTATGA GGCGGTGGAT GGTACCCGAG AATCCAGCAA TAGTGAGAGA
    GCAGCCAGCG ATGGGAAACC TGTAGGCATA GCCGCAGACA TCCCAGGTCT ACATCTGCCA
    GGTGGAGGGC GGCTGCCAGC AGAACATGGG AAGCCAGGAT GCAGTGATCT GGAGGAAGCT
    GCTGACTCTG GGGCTGACAA AAAGTTTACC CGGAAGCCAC CTCGATTTTC CTGTCTCCTG
    CCAGATCCAC GGGAACTGAT TGAAATTAAA AACAAAAAGA AGCTGCTAAT CACTGGCACA
    GAGCAGTTCA ACCAGAAACC AAAGAAGGGA ATCCAGTTTC TGCAGGAGAA AGGCCTCCTC
    ACCATCCCAA TGGACAACAC AGAGGTAGCC CAGTGGCTCC GAGAGAACCC TCGGCTGGAC
    AAGAAAATGA TTGGAGAGTT TGTGAGTGAC CGCAAAAACA TTGACCTGTT GGAGAGCTTT
    GTGAGCACCT TCAGCTTTCA GGGTCTGCGG CTGGATGAAG CTCTTCGTCT CTACCTGGAA
    GCCTTTCGCT TACCTGGGGA AGCACCAGTC ATCCAGAGGT TGCTGGAGGC ATTCACAGAG
    CATTGGAGGA ATTGTAATGG CTCCCCATTT GCCAATAGCG ATGCCTGCTT TGCTCTGGCC
    TATGCTGTCA TCTTGCTTAA TACTGACCAG CACAACCACA ACGTTCGCAA ACAGAATGCA
    CCCATGACTC TAGAGGAGTT TCGCAAAAAC CTAAAAGGTG TGAATGGAGG CAAGGACTTT
    GAGCAAGACA TCCTGGAGGA CATGTACCAT GCCATCAAGA ATGAGGAAAT TGTGATGCCT
    GAAGAGCAGA CAGGCTTGGT TCGGGAGAAC TATGTGTGGA ATGTGCTACT TCATCGAGGT
    GCCACCCCAG AGGGCATATT CCTTCGTGTG CCTGCTGGCA GCTATGATCT TGACCTCTTC
    ACCATGACGT GGGGCCCCAC TATTGCTGCC CTTTCTTATG TCTTTGACAA AAGCCTTGAG
    GAAACAATTA TCCAGAAAGC CATCTCAGGC TTCAGGAAGT GCGCCATGAT CTCCGCCCAC
    TATGGCCTCA GCGATGTGTT TGACAATCTC ATCATATCTC TGTGCAAATT CACAGCTCTT
    AGCAGTGAGT CTATTGAGAA TCTGCCCAGT GTGTTTGGAA GCAACCCCAA AGCCCACATT
    GCAGCCAAGA CAGTATTCCA TTTGGCCCAT CGTCATGGTG ATATCCTGCG GGAGGGCTGG
    AAGAATATCA TGGAAGCCAT GCTGCAGCTC TTCCGAGCCC AACTGCTTCC CAAAGCTATG
    GTGGAGGTAG AAGATTTTGT GGATCCCAAT GGCAAGATCT CTCTACAGAG GGAGGAGACA
    CCATCAAACC GAGGAGAGTC GACAGTGCTA AGCTTTGTGA GCTGGCTAAC ACTAAGTGGT
    ACTGAGCAGT CTAGTGTGCG GGGCCCATCC ACTGAGAACC AAGAGGCCAA AAGAGCGGCC
    TTGGACTGCA TCAAGCATTG TGACCCAGAA AAGATGATCA CAGAAAGCAA GTTCCTTCAG
    CTGGAGTCAC TGCAGGAGCT CATGAAGGCT CTGGTCTCAG TGACGCCAGA TGAAGAGACA
    TATGATGAGG AGGATGCTGC TTTCTGCCTA GAGATGCTGC TGAGGATTGT GCTGGAGAAC
    AGGGACCGTG TGGGTTGTGT GTGGCAGACT GTCCGAGACC ATCTATATCA CCTGTGTGTC
    CAGGCACAGG ATTTCTGCTT CCTTGTGGAG CGGGCAGTGG TGGGGCTGCT CCGCTTGGCC
    ATTCGACTAC TCCGAAGAGA AGAGATCAGT GGCCAGGTGC TGCTCTCCCT GCGTATTTTG
    CTACTAATGA AGCCCAGCGT GCTGTCCCGA GTCAGTCACC AGGTAGCCTA TGGGCTCCAT
    GAACTTCTTA AGACCAACGC AGCCAACATC CACTCAGGTG ACGACTGGGC CACTCTCTTC
    ACACTGCTGG AATGCATTGG CTCAGGTGTA AAGCCTCCAG CTGCCCTGCA GGCCACAGCC
    AGGGCTGAAG CACCTGATGC CGGGGCCCAA TCAGACAGCG AACTCCCATC CTACCATCAG
    AATGATGTGA GCCTGGACCG AGGATACACT TCTGACTCGG AGGTCTATGC TGACCATTGC
    AGGCCTGGCA AGATCCACCG ATCAGCCACT GATGCTGATG TAGTCAACAG CGGCTGGTTA
    GTGGTGGGGA AGGATGACAT TGATAACTCC AAGCCAGGTC CTGGGCCCAG CCGGCTAGGC
    CCTTCACCCC TGGTCAATCA GTACAGCCTA ACAGTGGGGC TGGACCTCGG GCCACACGAC
    ACTAAGTCCC TGCTTAAATG TGTAGAATCT CTGTCCTTCA TTGTGCGTGA TGCTGCCCAC
    ATTACACCTG ACAACTTTGA GCTCTGCGTG AAGACTCTCC GTATCTTTGT GGAGGCCAGT
    CTGAATGGCG GGTGCAAGTC CCAGGAGAAA CGTGGCAAGA GTCACAAATA TGACAGCAAA
    GGAAACCGCT TCAAGAAGAA ATCCAAGGAA GGTTCAGTGC TTCGGCGGCC TCGAACCTCT
    GGCCAACATG CCACTCGGGG TGGGCATAGT GACGATGATG AGGATGAAGG GGTGCCTGCC
    AGCTACCATA CGGTGTCTTT ACAGGTCAGT CAGGACTTGT TAGATCTGAT GCACACCCTG
    CACACGCGAG CAGCCTCTAT CTACAGCTCT TGGGCGGAGG AACAGCGCCA CCTAGAGACA
    GGTGGCCGGA AGATAGAAGC GGATTCACGT ACCCTCTGGG CCCACTGCTG GTGTCCCTTA
    CTGCAGGGCA TTGCCTGCTT GTGCTGTGAT GCCCGGCGCC AGGTGCGGAT GCAAGCACTG
    ACTTATCTGC AACGAGCACT ACTGGTACAT GATCTGCAGA AGCTAGATGC CCTGGAGTGG
    GAGTCCTGTT TTAACAAGGT GCTGTTTCCT CTACTGACCA AACTCTTGGA GAACATCAGC
    CCTGCAGATG TGGGTGGGAT GGAGGAGACT CGAATGAGGG CTTCCACACT GCTGTCTAAG
    GTCTTCCTGC AGCACCTGTC TCCACTGCTG TCACTCTCTA CCTTTGCGGC CCTCTGGCTG
    ACCATCCTGG ACTTCATGGA CAAGTATATG CATGCAGGAT CCAGTGACTT ACTGTCAGAG
    GCCATCCCTG AGTCTCTGAA GAACATGCTC CTGGTGATGG ACACAGCAGA GATTTTCCAC
    AATGCAGATG CCCGAGGAGG CAGCCCCTCA GCCCTCTGGG AGATCACCTG GGAGCGCATT
    GACTGTTTTC TGCCCCACCT ACGAGATGAG CTCTTCAAGC AGACTGTCAT CCAGGACCCC
    ATGCCACTGG AGCCACATGC CCAAAAAACT CTGGCCTCAG CCCACATGAC TCCTGCTGCT
    GGAGACACAA GGACACCTGG CCATCCACCT CCCCCAGAGA TGCCTTCTGA GCTGGGGGCC
    TGTGACTTCG AGAAGCCTGA GGGCCCTCGA CCTGCCAACA GCAGCTCTCC TGGATCACCA
    GTGGCATCTA GCCCCAACAG ACTGAGCCCT ACTTCAGATG GGCCTCCTCC TCTGGCTCAG
    CCTCCACTGA TCCTGCAGCC CTTGGCCTCC CCATTGCAGG TGGGCGTACC ACCCATGACT
    CTACCAATCA TCCTCAACCC TGCTCTCATT GAGGCCACTT CACCAGTGCC CCTCCTGGCC
    ACACCCCGCC CCACAGACCC AATGCCCACC TCCGAGGTCA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27947
    Clone ID Related Accession (Same CDS sequence) XM_005637657.3 , XM_005637657.2
    Accession Version XM_005637657.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5685bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726109.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005637657.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
    AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA
    CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT
    CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGA TTTCTCCATG TCTCAACAAA
    CTGGAGCTAA GTAGTGGGGA GCAGACCAAA GCCCTGAACC AGTTAGAGAG GGTACTACTC
    TTTAAGAACC TCAAGCTGAA AATGAGAGCA GGAGGCATGA GTGATTCATC CAAGTGGAAG
    AAACAGAAGA GATCCCCCCG GCCCCTGCGC CATATGACCA AAGTAACATC AGGTTCAGAG
    TTGCCCACCT CCAATGGAAC CACCTTATCC TCTAACCTCA CTGGGGGCAT GCCCTTCATT
    GATGTGCCCA CTCCCATCTC CTCTGCAAGT TCAGAAGCTG CCTCAACGGT GGTCAGCCCC
    TCTACAGACA GTGGCCTGGA GTTCTCCTCT CAGACCACCT CCAAGGAGGA CCTCACTGAC
    CTGGAGCAAC CTGGCTCTCC AGGGTATAGC ACAGCTACAG AGCCTGGTAG CAGTGAGCTA
    GGGGTTCCTG AGCAGCCTGA CCCGCAGCAG GAAGGAGTCC ATGTGGAAAA GGCCCAGTCA
    GCATCTGTGG AATCCATCCC CGAAGTGTTA GAGGAGTGCA CGTCTCCTGC TGACCACTCT
    GACTCTGCCT CTGTCCATGA CATGGATTAC GTCAATCCCC GGGGTGTGCG CTTTACACAG
    TCCTCCCAGA AAGAAGGTAC AGCTTTGGTT CCCTATGGTC TTCCCTGCAT CCGTGAGCTC
    TTCCGCTTCC TCATCTCCCT CACCAATCCA CATGACCGCC ACAATTCAGA AGTTATGATC
    CACATGGGAC TGCATTTGCT GACAGTGGCT CTTGAATCTG CCCCTGTAGC CCAGTGCCAA
    ACCCTTTTGG GCCTCATCAA AGATGAGATG TGCCGCCACT TACTCCAGCT ACTCAGTGTA
    GAGCGACTCA ACCTTTATGC TGCTTCCCTG CGGGTATGCT TCCTGCTTTT TGAGAGCATG
    AGGGAACATC TCAAGTTCCA ATTGGAGATG TACATCAAAA AGCTCATGGA GATCATCACT
    GTGGAGAACC CCAAAATGCC TTATGAGATG AAAGAGATGG CACTGGAGGC CATTGTGCAG
    CTCTGGCACA TCCCCAGTTT TGTCACTGAG CTCTATATCA ACTATGATTG TGACTACTAC
    TGTTCAAACC TCTTTGAAGA CCTCACCAAG CTGCTGTCCA AGAATGCCTT CCCTGTGTCT
    GGTCAGCTCT ATACAACACA CCTCCTGTCT CTTGATGCCC TGTTGACAGT GATTGACAGC
    ACTGAGGCCC ATTGCCAGGC CAAAGTCCTC AACAGTCTTA CCCAGCAAGA GAAGAAAGAA
    GCAGCCAGAT CTGGCTATGA GGCGGTGGAT GGTACCCGAG AATCCAGCAA TAGTGAGAGA
    GCAGCCAGCG ATGGGAAACC TGTAGGCATA GCCGCAGACA TCCCAGGTCT ACATCTGCCA
    GGTGGAGGGC GGCTGCCAGC AGAACATGGG AAGCCAGGAT GCAGTGATCT GGAGGAAGCT
    GCTGACTCTG GGGCTGACAA AAAGTTTACC CGGAAGCCAC CTCGATTTTC CTGTCTCCTG
    CCAGATCCAC GGGAACTGAT TGAAATTAAA AACAAAAAGA AGCTGCTAAT CACTGGCACA
    GAGCAGTTCA ACCAGAAACC AAAGAAGGGA ATCCAGTTTC TGCAGGAGAA AGGCCTCCTC
    ACCATCCCAA TGGACAACAC AGAGGTAGCC CAGTGGCTCC GAGAGAACCC TCGGCTGGAC
    AAGAAAATGA TTGGAGAGTT TGTGAGTGAC CGCAAAAACA TTGACCTGTT GGAGAGCTTT
    GTGAGCACCT TCAGCTTTCA GGGTCTGCGG CTGGATGAAG CTCTTCGTCT CTACCTGGAA
    GCCTTTCGCT TACCTGGGGA AGCACCAGTC ATCCAGAGGT TGCTGGAGGC ATTCACAGAG
    CATTGGAGGA ATTGTAATGG CTCCCCATTT GCCAATAGCG ATGCCTGCTT TGCTCTGGCC
    TATGCTGTCA TCTTGCTTAA TACTGACCAG CACAACCACA ACGTTCGCAA ACAGAATGCA
    CCCATGACTC TAGAGGAGTT TCGCAAAAAC CTAAAAGGTG TGAATGGAGG CAAGGACTTT
    GAGCAAGACA TCCTGGAGGA CATGTACCAT GCCATCAAGA ATGAGGAAAT TGTGATGCCT
    GAAGAGCAGA CAGGCTTGGT TCGGGAGAAC TATGTGTGGA ATGTGCTACT TCATCGAGGT
    GCCACCCCAG AGGGCATATT CCTTCGTGTG CCTGCTGGCA GCTATGATCT TGACCTCTTC
    ACCATGACGT GGGGCCCCAC TATTGCTGCC CTTTCTTATG TCTTTGACAA AAGCCTTGAG
    GAAACAATTA TCCAGAAAGC CATCTCAGGC TTCAGGAAGT GCGCCATGAT CTCCGCCCAC
    TATGGCCTCA GCGATGTGTT TGACAATCTC ATCATATCTC TGTGCAAATT CACAGCTCTT
    AGCAGTGAGT CTATTGAGAA TCTGCCCAGT GTGTTTGGAA GCAACCCCAA AGCCCACATT
    GCAGCCAAGA CAGTATTCCA TTTGGCCCAT CGTCATGGTG ATATCCTGCG GGAGGGCTGG
    AAGAATATCA TGGAAGCCAT GCTGCAGCTC TTCCGAGCCC AACTGCTTCC CAAAGCTATG
    GTGGAGGTAG AAGATTTTGT GGATCCCAAT GGCAAGATCT CTCTACAGAG GGAGGAGACA
    CCATCAAACC GAGGAGAGTC GACAGTGCTA AGCTTTGTGA GCTGGCTAAC ACTAAGTGGT
    ACTGAGCAGT CTAGTGTGCG GGGCCCATCC ACTGAGAACC AAGAGGCCAA AAGAGCGGCC
    TTGGACTGCA TCAAGCATTG TGACCCAGAA AAGATGATCA CAGAAAGCAA GTTCCTTCAG
    CTGGAGTCAC TGCAGGAGCT CATGAAGGCT CTGGTCTCAG TGACGCCAGA TGAAGAGACA
    TATGATGAGG AGGATGCTGC TTTCTGCCTA GAGATGCTGC TGAGGATTGT GCTGGAGAAC
    AGGGACCGTG TGGGTTGTGT GTGGCAGACT GTCCGAGACC ATCTATATCA CCTGTGTGTC
    CAGGCACAGG ATTTCTGCTT CCTTGTGGAG CGGGCAGTGG TGGGGCTGCT CCGCTTGGCC
    ATTCGACTAC TCCGAAGAGA AGAGATCAGT GGCCAGGTGC TGCTCTCCCT GCGTATTTTG
    CTACTAATGA AGCCCAGCGT GCTGTCCCGA GTCAGTCACC AGGTAGCCTA TGGGCTCCAT
    GAACTTCTTA AGACCAACGC AGCCAACATC CACTCAGGTG ACGACTGGGC CACTCTCTTC
    ACACTGCTGG AATGCATTGG CTCAGGTGTA AAGCCTCCAG CTGCCCTGCA GGCCACAGCC
    AGGGCTGAAG CACCTGATGC CGGGGCCCAA TCAGACAGCG AACTCCCATC CTACCATCAG
    AATGATGTGA GCCTGGACCG AGGATACACT TCTGACTCGG AGGTCTATGC TGACCATTGC
    AGGCCTGGCA AGATCCACCG ATCAGCCACT GATGCTGATG TAGTCAACAG CGGCTGGTTA
    GTGGTGGGGA AGGATGACAT TGATAACTCC AAGCCAGGTC CTGGGCCCAG CCGGCTAGGC
    CCTTCACCCC TGGTCAATCA GTACAGCCTA ACAGTGGGGC TGGACCTCGG GCCACACGAC
    ACTAAGTCCC TGCTTAAATG TGTAGAATCT CTGTCCTTCA TTGTGCGTGA TGCTGCCCAC
    ATTACACCTG ACAACTTTGA GCTCTGCGTG AAGACTCTCC GTATCTTTGT GGAGGCCAGT
    CTGAATGGCG GGTGCAAGTC CCAGGAGAAA CGTGGCAAGA GTCACAAATA TGACAGCAAA
    GGAAACCGCT TCAAGAAGAA ATCCAAGGAA GGTTCAGTGC TTCGGCGGCC TCGAACCTCT
    GGCCAACATG CCACTCGGGG TGGGCATAGT GACGATGATG AGGATGAAGG GGTGCCTGCC
    AGCTACCATA CGGTGTCTTT ACAGGTCAGT CAGGACTTGT TAGATCTGAT GCACACCCTG
    CACACGCGAG CAGCCTCTAT CTACAGCTCT TGGGCGGAGG AACAGCGCCA CCTAGAGACA
    GGTGGCCGGA AGATAGAAGC GGATTCACGT ACCCTCTGGG CCCACTGCTG GTGTCCCTTA
    CTGCAGGGCA TTGCCTGCTT GTGCTGTGAT GCCCGGCGCC AGGTGCGGAT GCAAGCACTG
    ACTTATCTGC AACGAGCACT ACTGGTACAT GATCTGCAGA AGCTAGATGC CCTGGAGTGG
    GAGTCCTGTT TTAACAAGGT GCTGTTTCCT CTACTGACCA AACTCTTGGA GAACATCAGC
    CCTGCAGATG TGGGTGGGAT GGAGGAGACT CGAATGAGGG CTTCCACACT GCTGTCTAAG
    GTCTTCCTGC AGCACCTGTC TCCACTGCTG TCACTCTCTA CCTTTGCGGC CCTCTGGCTG
    ACCATCCTGG ACTTCATGGA CAAGTATATG CATGCAGGAT CCAGTGACTT ACTGTCAGAG
    GCCATCCCTG AGTCTCTGAA GAACATGCTC CTGGTGATGG ACACAGCAGA GATTTTCCAC
    AATGCAGATG CCCGAGGAGG CAGCCCCTCA GCCCTCTGGG AGATCACCTG GGAGCGCATT
    GACTGTTTTC TGCCCCACCT ACGAGATGAG CTCTTCAAGC AGACTGTCAT CCAGGACCCC
    ATGCCACTGG AGCCACATGC CCAAAAAACT CTGGCCTCAG CCCACATGAC TCCTGCTGCT
    GGAGACACAA GGACACCTGG CCATCCACCT CCCCCAGAGA TGCCTTCTGA GCTGGGGGCC
    TGTGACTTCG AGAAGCCTGA GGGCCCTCGA CCTGCCAACA GCAGCTCTCC TGGATCACCA
    GTGGCATCTA GCCCCAACAG ACTGAGCCCT ACTTCAGATG GGCCTCCTCC TCTGGCTCAG
    CCTCCACTGA TCCTGCAGCC CTTGGCCTCC CCATTGCAGG TGGGCGTACC ACCCATGACT
    CTACCAATCA TCCTCAACCC TGCTCTCATT GAGGCCACTT CACCAGTGCC CCTCCTGGCC
    ACACCCCGCC CCACAGACCC AATGCCCACC TCCGAGGTCA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005637714.1
    CDS251..5935
    Translation

    Target ORF information:

    RefSeq Version XM_005637657.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005637657.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
    AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA
    CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT
    CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGA TTTCTCCATG TCTCAACAAA
    CTGGAGCTAA GTAGTGGGGA GCAGACCAAA GCCCTGAACC AGTTAGAGAG GGTACTACTC
    TTTAAGAACC TCAAGCTGAA AATGAGAGCA GGAGGCATGA GTGATTCATC CAAGTGGAAG
    AAACAGAAGA GATCCCCCCG GCCCCTGCGC CATATGACCA AAGTAACATC AGGTTCAGAG
    TTGCCCACCT CCAATGGAAC CACCTTATCC TCTAACCTCA CTGGGGGCAT GCCCTTCATT
    GATGTGCCCA CTCCCATCTC CTCTGCAAGT TCAGAAGCTG CCTCAACGGT GGTCAGCCCC
    TCTACAGACA GTGGCCTGGA GTTCTCCTCT CAGACCACCT CCAAGGAGGA CCTCACTGAC
    CTGGAGCAAC CTGGCTCTCC AGGGTATAGC ACAGCTACAG AGCCTGGTAG CAGTGAGCTA
    GGGGTTCCTG AGCAGCCTGA CCCGCAGCAG GAAGGAGTCC ATGTGGAAAA GGCCCAGTCA
    GCATCTGTGG AATCCATCCC CGAAGTGTTA GAGGAGTGCA CGTCTCCTGC TGACCACTCT
    GACTCTGCCT CTGTCCATGA CATGGATTAC GTCAATCCCC GGGGTGTGCG CTTTACACAG
    TCCTCCCAGA AAGAAGGTAC AGCTTTGGTT CCCTATGGTC TTCCCTGCAT CCGTGAGCTC
    TTCCGCTTCC TCATCTCCCT CACCAATCCA CATGACCGCC ACAATTCAGA AGTTATGATC
    CACATGGGAC TGCATTTGCT GACAGTGGCT CTTGAATCTG CCCCTGTAGC CCAGTGCCAA
    ACCCTTTTGG GCCTCATCAA AGATGAGATG TGCCGCCACT TACTCCAGCT ACTCAGTGTA
    GAGCGACTCA ACCTTTATGC TGCTTCCCTG CGGGTATGCT TCCTGCTTTT TGAGAGCATG
    AGGGAACATC TCAAGTTCCA ATTGGAGATG TACATCAAAA AGCTCATGGA GATCATCACT
    GTGGAGAACC CCAAAATGCC TTATGAGATG AAAGAGATGG CACTGGAGGC CATTGTGCAG
    CTCTGGCACA TCCCCAGTTT TGTCACTGAG CTCTATATCA ACTATGATTG TGACTACTAC
    TGTTCAAACC TCTTTGAAGA CCTCACCAAG CTGCTGTCCA AGAATGCCTT CCCTGTGTCT
    GGTCAGCTCT ATACAACACA CCTCCTGTCT CTTGATGCCC TGTTGACAGT GATTGACAGC
    ACTGAGGCCC ATTGCCAGGC CAAAGTCCTC AACAGTCTTA CCCAGCAAGA GAAGAAAGAA
    GCAGCCAGAT CTGGCTATGA GGCGGTGGAT GGTACCCGAG AATCCAGCAA TAGTGAGAGA
    GCAGCCAGCG ATGGGAAACC TGTAGGCATA GCCGCAGACA TCCCAGGTCT ACATCTGCCA
    GGTGGAGGGC GGCTGCCAGC AGAACATGGG AAGCCAGGAT GCAGTGATCT GGAGGAAGCT
    GCTGACTCTG GGGCTGACAA AAAGTTTACC CGGAAGCCAC CTCGATTTTC CTGTCTCCTG
    CCAGATCCAC GGGAACTGAT TGAAATTAAA AACAAAAAGA AGCTGCTAAT CACTGGCACA
    GAGCAGTTCA ACCAGAAACC AAAGAAGGGA ATCCAGTTTC TGCAGGAGAA AGGCCTCCTC
    ACCATCCCAA TGGACAACAC AGAGGTAGCC CAGTGGCTCC GAGAGAACCC TCGGCTGGAC
    AAGAAAATGA TTGGAGAGTT TGTGAGTGAC CGCAAAAACA TTGACCTGTT GGAGAGCTTT
    GTGAGCACCT TCAGCTTTCA GGGTCTGCGG CTGGATGAAG CTCTTCGTCT CTACCTGGAA
    GCCTTTCGCT TACCTGGGGA AGCACCAGTC ATCCAGAGGT TGCTGGAGGC ATTCACAGAG
    CATTGGAGGA ATTGTAATGG CTCCCCATTT GCCAATAGCG ATGCCTGCTT TGCTCTGGCC
    TATGCTGTCA TCTTGCTTAA TACTGACCAG CACAACCACA ACGTTCGCAA ACAGAATGCA
    CCCATGACTC TAGAGGAGTT TCGCAAAAAC CTAAAAGGTG TGAATGGAGG CAAGGACTTT
    GAGCAAGACA TCCTGGAGGA CATGTACCAT GCCATCAAGA ATGAGGAAAT TGTGATGCCT
    GAAGAGCAGA CAGGCTTGGT TCGGGAGAAC TATGTGTGGA ATGTGCTACT TCATCGAGGT
    GCCACCCCAG AGGGCATATT CCTTCGTGTG CCTGCTGGCA GCTATGATCT TGACCTCTTC
    ACCATGACGT GGGGCCCCAC TATTGCTGCC CTTTCTTATG TCTTTGACAA AAGCCTTGAG
    GAAACAATTA TCCAGAAAGC CATCTCAGGC TTCAGGAAGT GCGCCATGAT CTCCGCCCAC
    TATGGCCTCA GCGATGTGTT TGACAATCTC ATCATATCTC TGTGCAAATT CACAGCTCTT
    AGCAGTGAGT CTATTGAGAA TCTGCCCAGT GTGTTTGGAA GCAACCCCAA AGCCCACATT
    GCAGCCAAGA CAGTATTCCA TTTGGCCCAT CGTCATGGTG ATATCCTGCG GGAGGGCTGG
    AAGAATATCA TGGAAGCCAT GCTGCAGCTC TTCCGAGCCC AACTGCTTCC CAAAGCTATG
    GTGGAGGTAG AAGATTTTGT GGATCCCAAT GGCAAGATCT CTCTACAGAG GGAGGAGACA
    CCATCAAACC GAGGAGAGTC GACAGTGCTA AGCTTTGTGA GCTGGCTAAC ACTAAGTGGT
    ACTGAGCAGT CTAGTGTGCG GGGCCCATCC ACTGAGAACC AAGAGGCCAA AAGAGCGGCC
    TTGGACTGCA TCAAGCATTG TGACCCAGAA AAGATGATCA CAGAAAGCAA GTTCCTTCAG
    CTGGAGTCAC TGCAGGAGCT CATGAAGGCT CTGGTCTCAG TGACGCCAGA TGAAGAGACA
    TATGATGAGG AGGATGCTGC TTTCTGCCTA GAGATGCTGC TGAGGATTGT GCTGGAGAAC
    AGGGACCGTG TGGGTTGTGT GTGGCAGACT GTCCGAGACC ATCTATATCA CCTGTGTGTC
    CAGGCACAGG ATTTCTGCTT CCTTGTGGAG CGGGCAGTGG TGGGGCTGCT CCGCTTGGCC
    ATTCGACTAC TCCGAAGAGA AGAGATCAGT GGCCAGGTGC TGCTCTCCCT GCGTATTTTG
    CTACTAATGA AGCCCAGCGT GCTGTCCCGA GTCAGTCACC AGGTAGCCTA TGGGCTCCAT
    GAACTTCTTA AGACCAACGC AGCCAACATC CACTCAGGTG ACGACTGGGC CACTCTCTTC
    ACACTGCTGG AATGCATTGG CTCAGGTGTA AAGCCTCCAG CTGCCCTGCA GGCCACAGCC
    AGGGCTGAAG CACCTGATGC CGGGGCCCAA TCAGACAGCG AACTCCCATC CTACCATCAG
    AATGATGTGA GCCTGGACCG AGGATACACT TCTGACTCGG AGGTCTATGC TGACCATTGC
    AGGCCTGGCA AGATCCACCG ATCAGCCACT GATGCTGATG TAGTCAACAG CGGCTGGTTA
    GTGGTGGGGA AGGATGACAT TGATAACTCC AAGCCAGGTC CTGGGCCCAG CCGGCTAGGC
    CCTTCACCCC TGGTCAATCA GTACAGCCTA ACAGTGGGGC TGGACCTCGG GCCACACGAC
    ACTAAGTCCC TGCTTAAATG TGTAGAATCT CTGTCCTTCA TTGTGCGTGA TGCTGCCCAC
    ATTACACCTG ACAACTTTGA GCTCTGCGTG AAGACTCTCC GTATCTTTGT GGAGGCCAGT
    CTGAATGGCG GGTGCAAGTC CCAGGAGAAA CGTGGCAAGA GTCACAAATA TGACAGCAAA
    GGAAACCGCT TCAAGAAGAA ATCCAAGGAA GGTTCAGTGC TTCGGCGGCC TCGAACCTCT
    GGCCAACATG CCACTCGGGG TGGGCATAGT GACGATGATG AGGATGAAGG GGTGCCTGCC
    AGCTACCATA CGGTGTCTTT ACAGGTCAGT CAGGACTTGT TAGATCTGAT GCACACCCTG
    CACACGCGAG CAGCCTCTAT CTACAGCTCT TGGGCGGAGG AACAGCGCCA CCTAGAGACA
    GGTGGCCGGA AGATAGAAGC GGATTCACGT ACCCTCTGGG CCCACTGCTG GTGTCCCTTA
    CTGCAGGGCA TTGCCTGCTT GTGCTGTGAT GCCCGGCGCC AGGTGCGGAT GCAAGCACTG
    ACTTATCTGC AACGAGCACT ACTGGTACAT GATCTGCAGA AGCTAGATGC CCTGGAGTGG
    GAGTCCTGTT TTAACAAGGT GCTGTTTCCT CTACTGACCA AACTCTTGGA GAACATCAGC
    CCTGCAGATG TGGGTGGGAT GGAGGAGACT CGAATGAGGG CTTCCACACT GCTGTCTAAG
    GTCTTCCTGC AGCACCTGTC TCCACTGCTG TCACTCTCTA CCTTTGCGGC CCTCTGGCTG
    ACCATCCTGG ACTTCATGGA CAAGTATATG CATGCAGGAT CCAGTGACTT ACTGTCAGAG
    GCCATCCCTG AGTCTCTGAA GAACATGCTC CTGGTGATGG ACACAGCAGA GATTTTCCAC
    AATGCAGATG CCCGAGGAGG CAGCCCCTCA GCCCTCTGGG AGATCACCTG GGAGCGCATT
    GACTGTTTTC TGCCCCACCT ACGAGATGAG CTCTTCAAGC AGACTGTCAT CCAGGACCCC
    ATGCCACTGG AGCCACATGC CCAAAAAACT CTGGCCTCAG CCCACATGAC TCCTGCTGCT
    GGAGACACAA GGACACCTGG CCATCCACCT CCCCCAGAGA TGCCTTCTGA GCTGGGGGCC
    TGTGACTTCG AGAAGCCTGA GGGCCCTCGA CCTGCCAACA GCAGCTCTCC TGGATCACCA
    GTGGCATCTA GCCCCAACAG ACTGAGCCCT ACTTCAGATG GGCCTCCTCC TCTGGCTCAG
    CCTCCACTGA TCCTGCAGCC CTTGGCCTCC CCATTGCAGG TGGGCGTACC ACCCATGACT
    CTACCAATCA TCCTCAACCC TGCTCTCATT GAGGCCACTT CACCAGTGCC CCTCCTGGCC
    ACACCCCGCC CCACAGACCC AATGCCCACC TCCGAGGTCA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27948
    Clone ID Related Accession (Same CDS sequence) XM_005637658.3 , XM_005637658.2
    Accession Version XM_005637658.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5682bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006610.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005637658.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
    AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA
    CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT
    CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGA TTTCTCCATG TCTCAACAAA
    CTGGAGCTAA GTAGTGGGGA GCAGACCAAA GCCCTGAACC AGTTAGAGAG GGTACTACTC
    TTTAAGAACC TCAAGCTGAA AATGAGAGCA GGAGGCATGA GTGATTCATC CAAGTGGAAG
    AAACAGAAGA GATCCCCCCG GCCCCTGCGC CATATGACCA AAGTAACATC AGGTTCAGAG
    TTGCCCACCT CCAATGGAAC CACCTTATCC TCTAACCTCA CTGGGGGCAT GCCCTTCATT
    GATGTGCCCA CTCCCATCTC CTCTGCAAGT TCAGAAGCTG CCTCAACGGT GGTCAGCCCC
    TCTACAGACA GTGGCCTGGA GTTCTCCTCT CAGACCACCT CCAAGGAGGA CCTCACTGAC
    CTGGAGCAAC CTGGCTCTCC AGGGTATAGC ACAGCTACAG AGCCTGGTAG CAGTGAGCTA
    GGGGTTCCTG AGCAGCCTGA CCCGCAGGAA GGAGTCCATG TGGAAAAGGC CCAGTCAGCA
    TCTGTGGAAT CCATCCCCGA AGTGTTAGAG GAGTGCACGT CTCCTGCTGA CCACTCTGAC
    TCTGCCTCTG TCCATGACAT GGATTACGTC AATCCCCGGG GTGTGCGCTT TACACAGTCC
    TCCCAGAAAG AAGGTACAGC TTTGGTTCCC TATGGTCTTC CCTGCATCCG TGAGCTCTTC
    CGCTTCCTCA TCTCCCTCAC CAATCCACAT GACCGCCACA ATTCAGAAGT TATGATCCAC
    ATGGGACTGC ATTTGCTGAC AGTGGCTCTT GAATCTGCCC CTGTAGCCCA GTGCCAAACC
    CTTTTGGGCC TCATCAAAGA TGAGATGTGC CGCCACTTAC TCCAGCTACT CAGTGTAGAG
    CGACTCAACC TTTATGCTGC TTCCCTGCGG GTATGCTTCC TGCTTTTTGA GAGCATGAGG
    GAACATCTCA AGTTCCAATT GGAGATGTAC ATCAAAAAGC TCATGGAGAT CATCACTGTG
    GAGAACCCCA AAATGCCTTA TGAGATGAAA GAGATGGCAC TGGAGGCCAT TGTGCAGCTC
    TGGCACATCC CCAGTTTTGT CACTGAGCTC TATATCAACT ATGATTGTGA CTACTACTGT
    TCAAACCTCT TTGAAGACCT CACCAAGCTG CTGTCCAAGA ATGCCTTCCC TGTGTCTGGT
    CAGCTCTATA CAACACACCT CCTGTCTCTT GATGCCCTGT TGACAGTGAT TGACAGCACT
    GAGGCCCATT GCCAGGCCAA AGTCCTCAAC AGTCTTACCC AGCAAGAGAA GAAAGAAGCA
    GCCAGATCTG GCTATGAGGC GGTGGATGGT ACCCGAGAAT CCAGCAATAG TGAGAGAGCA
    GCCAGCGATG GGAAACCTGT AGGCATAGCC GCAGACATCC CAGGTCTACA TCTGCCAGGT
    GGAGGGCGGC TGCCAGCAGA ACATGGGAAG CCAGGATGCA GTGATCTGGA GGAAGCTGCT
    GACTCTGGGG CTGACAAAAA GTTTACCCGG AAGCCACCTC GATTTTCCTG TCTCCTGCCA
    GATCCACGGG AACTGATTGA AATTAAAAAC AAAAAGAAGC TGCTAATCAC TGGCACAGAG
    CAGTTCAACC AGAAACCAAA GAAGGGAATC CAGTTTCTGC AGGAGAAAGG CCTCCTCACC
    ATCCCAATGG ACAACACAGA GGTAGCCCAG TGGCTCCGAG AGAACCCTCG GCTGGACAAG
    AAAATGATTG GAGAGTTTGT GAGTGACCGC AAAAACATTG ACCTGTTGGA GAGCTTTGTG
    AGCACCTTCA GCTTTCAGGG TCTGCGGCTG GATGAAGCTC TTCGTCTCTA CCTGGAAGCC
    TTTCGCTTAC CTGGGGAAGC ACCAGTCATC CAGAGGTTGC TGGAGGCATT CACAGAGCAT
    TGGAGGAATT GTAATGGCTC CCCATTTGCC AATAGCGATG CCTGCTTTGC TCTGGCCTAT
    GCTGTCATCT TGCTTAATAC TGACCAGCAC AACCACAACG TTCGCAAACA GAATGCACCC
    ATGACTCTAG AGGAGTTTCG CAAAAACCTA AAAGGTGTGA ATGGAGGCAA GGACTTTGAG
    CAAGACATCC TGGAGGACAT GTACCATGCC ATCAAGAATG AGGAAATTGT GATGCCTGAA
    GAGCAGACAG GCTTGGTTCG GGAGAACTAT GTGTGGAATG TGCTACTTCA TCGAGGTGCC
    ACCCCAGAGG GCATATTCCT TCGTGTGCCT GCTGGCAGCT ATGATCTTGA CCTCTTCACC
    ATGACGTGGG GCCCCACTAT TGCTGCCCTT TCTTATGTCT TTGACAAAAG CCTTGAGGAA
    ACAATTATCC AGAAAGCCAT CTCAGGCTTC AGGAAGTGCG CCATGATCTC CGCCCACTAT
    GGCCTCAGCG ATGTGTTTGA CAATCTCATC ATATCTCTGT GCAAATTCAC AGCTCTTAGC
    AGTGAGTCTA TTGAGAATCT GCCCAGTGTG TTTGGAAGCA ACCCCAAAGC CCACATTGCA
    GCCAAGACAG TATTCCATTT GGCCCATCGT CATGGTGATA TCCTGCGGGA GGGCTGGAAG
    AATATCATGG AAGCCATGCT GCAGCTCTTC CGAGCCCAAC TGCTTCCCAA AGCTATGGTG
    GAGGTAGAAG ATTTTGTGGA TCCCAATGGC AAGATCTCTC TACAGAGGGA GGAGACACCA
    TCAAACCGAG GAGAGTCGAC AGTGCTAAGC TTTGTGAGCT GGCTAACACT AAGTGGTACT
    GAGCAGTCTA GTGTGCGGGG CCCATCCACT GAGAACCAAG AGGCCAAAAG AGCGGCCTTG
    GACTGCATCA AGCATTGTGA CCCAGAAAAG ATGATCACAG AAAGCAAGTT CCTTCAGCTG
    GAGTCACTGC AGGAGCTCAT GAAGGCTCTG GTCTCAGTGA CGCCAGATGA AGAGACATAT
    GATGAGGAGG ATGCTGCTTT CTGCCTAGAG ATGCTGCTGA GGATTGTGCT GGAGAACAGG
    GACCGTGTGG GTTGTGTGTG GCAGACTGTC CGAGACCATC TATATCACCT GTGTGTCCAG
    GCACAGGATT TCTGCTTCCT TGTGGAGCGG GCAGTGGTGG GGCTGCTCCG CTTGGCCATT
    CGACTACTCC GAAGAGAAGA GATCAGTGGC CAGGTGCTGC TCTCCCTGCG TATTTTGCTA
    CTAATGAAGC CCAGCGTGCT GTCCCGAGTC AGTCACCAGG TAGCCTATGG GCTCCATGAA
    CTTCTTAAGA CCAACGCAGC CAACATCCAC TCAGGTGACG ACTGGGCCAC TCTCTTCACA
    CTGCTGGAAT GCATTGGCTC AGGTGTAAAG CCTCCAGCTG CCCTGCAGGC CACAGCCAGG
    GCTGAAGCAC CTGATGCCGG GGCCCAATCA GACAGCGAAC TCCCATCCTA CCATCAGAAT
    GATGTGAGCC TGGACCGAGG ATACACTTCT GACTCGGAGG TCTATGCTGA CCATTGCAGG
    CCTGGCAAGA TCCACCGATC AGCCACTGAT GCTGATGTAG TCAACAGCGG CTGGTTAGTG
    GTGGGGAAGG ATGACATTGA TAACTCCAAG CCAGGTCCTG GGCCCAGCCG GCTAGGCCCT
    TCACCCCTGG TCAATCAGTA CAGCCTAACA GTGGGGCTGG ACCTCGGGCC ACACGACACT
    AAGTCCCTGC TTAAATGTGT AGAATCTCTG TCCTTCATTG TGCGTGATGC TGCCCACATT
    ACACCTGACA ACTTTGAGCT CTGCGTGAAG ACTCTCCGTA TCTTTGTGGA GGCCAGTCTG
    AATGGCGGGT GCAAGTCCCA GGAGAAACGT GGCAAGAGTC ACAAATATGA CAGCAAAGGA
    AACCGCTTCA AGAAGAAATC CAAGGAAGGT TCAGTGCTTC GGCGGCCTCG AACCTCTGGC
    CAACATGCCA CTCGGGGTGG GCATAGTGAC GATGATGAGG ATGAAGGGGT GCCTGCCAGC
    TACCATACGG TGTCTTTACA GGTCAGTCAG GACTTGTTAG ATCTGATGCA CACCCTGCAC
    ACGCGAGCAG CCTCTATCTA CAGCTCTTGG GCGGAGGAAC AGCGCCACCT AGAGACAGGT
    GGCCGGAAGA TAGAAGCGGA TTCACGTACC CTCTGGGCCC ACTGCTGGTG TCCCTTACTG
    CAGGGCATTG CCTGCTTGTG CTGTGATGCC CGGCGCCAGG TGCGGATGCA AGCACTGACT
    TATCTGCAAC GAGCACTACT GGTACATGAT CTGCAGAAGC TAGATGCCCT GGAGTGGGAG
    TCCTGTTTTA ACAAGGTGCT GTTTCCTCTA CTGACCAAAC TCTTGGAGAA CATCAGCCCT
    GCAGATGTGG GTGGGATGGA GGAGACTCGA ATGAGGGCTT CCACACTGCT GTCTAAGGTC
    TTCCTGCAGC ACCTGTCTCC ACTGCTGTCA CTCTCTACCT TTGCGGCCCT CTGGCTGACC
    ATCCTGGACT TCATGGACAA GTATATGCAT GCAGGATCCA GTGACTTACT GTCAGAGGCC
    ATCCCTGAGT CTCTGAAGAA CATGCTCCTG GTGATGGACA CAGCAGAGAT TTTCCACAAT
    GCAGATGCCC GAGGAGGCAG CCCCTCAGCC CTCTGGGAGA TCACCTGGGA GCGCATTGAC
    TGTTTTCTGC CCCACCTACG AGATGAGCTC TTCAAGCAGA CTGTCATCCA GGACCCCATG
    CCACTGGAGC CACATGCCCA AAAAACTCTG GCCTCAGCCC ACATGACTCC TGCTGCTGGA
    GACACAAGGA CACCTGGCCA TCCACCTCCC CCAGAGATGC CTTCTGAGCT GGGGGCCTGT
    GACTTCGAGA AGCCTGAGGG CCCTCGACCT GCCAACAGCA GCTCTCCTGG ATCACCAGTG
    GCATCTAGCC CCAACAGACT GAGCCCTACT TCAGATGGGC CTCCTCCTCT GGCTCAGCCT
    CCACTGATCC TGCAGCCCTT GGCCTCCCCA TTGCAGGTGG GCGTACCACC CATGACTCTA
    CCAATCATCC TCAACCCTGC TCTCATTGAG GCCACTTCAC CAGTGCCCCT CCTGGCCACA
    CCCCGCCCCA CAGACCCAAT GCCCACCTCC GAGGTCAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005637715.1
    CDS269..5950
    Translation

    Target ORF information:

    RefSeq Version XM_005637658.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005637658.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
    AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA
    CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT
    CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGA TTTCTCCATG TCTCAACAAA
    CTGGAGCTAA GTAGTGGGGA GCAGACCAAA GCCCTGAACC AGTTAGAGAG GGTACTACTC
    TTTAAGAACC TCAAGCTGAA AATGAGAGCA GGAGGCATGA GTGATTCATC CAAGTGGAAG
    AAACAGAAGA GATCCCCCCG GCCCCTGCGC CATATGACCA AAGTAACATC AGGTTCAGAG
    TTGCCCACCT CCAATGGAAC CACCTTATCC TCTAACCTCA CTGGGGGCAT GCCCTTCATT
    GATGTGCCCA CTCCCATCTC CTCTGCAAGT TCAGAAGCTG CCTCAACGGT GGTCAGCCCC
    TCTACAGACA GTGGCCTGGA GTTCTCCTCT CAGACCACCT CCAAGGAGGA CCTCACTGAC
    CTGGAGCAAC CTGGCTCTCC AGGGTATAGC ACAGCTACAG AGCCTGGTAG CAGTGAGCTA
    GGGGTTCCTG AGCAGCCTGA CCCGCAGGAA GGAGTCCATG TGGAAAAGGC CCAGTCAGCA
    TCTGTGGAAT CCATCCCCGA AGTGTTAGAG GAGTGCACGT CTCCTGCTGA CCACTCTGAC
    TCTGCCTCTG TCCATGACAT GGATTACGTC AATCCCCGGG GTGTGCGCTT TACACAGTCC
    TCCCAGAAAG AAGGTACAGC TTTGGTTCCC TATGGTCTTC CCTGCATCCG TGAGCTCTTC
    CGCTTCCTCA TCTCCCTCAC CAATCCACAT GACCGCCACA ATTCAGAAGT TATGATCCAC
    ATGGGACTGC ATTTGCTGAC AGTGGCTCTT GAATCTGCCC CTGTAGCCCA GTGCCAAACC
    CTTTTGGGCC TCATCAAAGA TGAGATGTGC CGCCACTTAC TCCAGCTACT CAGTGTAGAG
    CGACTCAACC TTTATGCTGC TTCCCTGCGG GTATGCTTCC TGCTTTTTGA GAGCATGAGG
    GAACATCTCA AGTTCCAATT GGAGATGTAC ATCAAAAAGC TCATGGAGAT CATCACTGTG
    GAGAACCCCA AAATGCCTTA TGAGATGAAA GAGATGGCAC TGGAGGCCAT TGTGCAGCTC
    TGGCACATCC CCAGTTTTGT CACTGAGCTC TATATCAACT ATGATTGTGA CTACTACTGT
    TCAAACCTCT TTGAAGACCT CACCAAGCTG CTGTCCAAGA ATGCCTTCCC TGTGTCTGGT
    CAGCTCTATA CAACACACCT CCTGTCTCTT GATGCCCTGT TGACAGTGAT TGACAGCACT
    GAGGCCCATT GCCAGGCCAA AGTCCTCAAC AGTCTTACCC AGCAAGAGAA GAAAGAAGCA
    GCCAGATCTG GCTATGAGGC GGTGGATGGT ACCCGAGAAT CCAGCAATAG TGAGAGAGCA
    GCCAGCGATG GGAAACCTGT AGGCATAGCC GCAGACATCC CAGGTCTACA TCTGCCAGGT
    GGAGGGCGGC TGCCAGCAGA ACATGGGAAG CCAGGATGCA GTGATCTGGA GGAAGCTGCT
    GACTCTGGGG CTGACAAAAA GTTTACCCGG AAGCCACCTC GATTTTCCTG TCTCCTGCCA
    GATCCACGGG AACTGATTGA AATTAAAAAC AAAAAGAAGC TGCTAATCAC TGGCACAGAG
    CAGTTCAACC AGAAACCAAA GAAGGGAATC CAGTTTCTGC AGGAGAAAGG CCTCCTCACC
    ATCCCAATGG ACAACACAGA GGTAGCCCAG TGGCTCCGAG AGAACCCTCG GCTGGACAAG
    AAAATGATTG GAGAGTTTGT GAGTGACCGC AAAAACATTG ACCTGTTGGA GAGCTTTGTG
    AGCACCTTCA GCTTTCAGGG TCTGCGGCTG GATGAAGCTC TTCGTCTCTA CCTGGAAGCC
    TTTCGCTTAC CTGGGGAAGC ACCAGTCATC CAGAGGTTGC TGGAGGCATT CACAGAGCAT
    TGGAGGAATT GTAATGGCTC CCCATTTGCC AATAGCGATG CCTGCTTTGC TCTGGCCTAT
    GCTGTCATCT TGCTTAATAC TGACCAGCAC AACCACAACG TTCGCAAACA GAATGCACCC
    ATGACTCTAG AGGAGTTTCG CAAAAACCTA AAAGGTGTGA ATGGAGGCAA GGACTTTGAG
    CAAGACATCC TGGAGGACAT GTACCATGCC ATCAAGAATG AGGAAATTGT GATGCCTGAA
    GAGCAGACAG GCTTGGTTCG GGAGAACTAT GTGTGGAATG TGCTACTTCA TCGAGGTGCC
    ACCCCAGAGG GCATATTCCT TCGTGTGCCT GCTGGCAGCT ATGATCTTGA CCTCTTCACC
    ATGACGTGGG GCCCCACTAT TGCTGCCCTT TCTTATGTCT TTGACAAAAG CCTTGAGGAA
    ACAATTATCC AGAAAGCCAT CTCAGGCTTC AGGAAGTGCG CCATGATCTC CGCCCACTAT
    GGCCTCAGCG ATGTGTTTGA CAATCTCATC ATATCTCTGT GCAAATTCAC AGCTCTTAGC
    AGTGAGTCTA TTGAGAATCT GCCCAGTGTG TTTGGAAGCA ACCCCAAAGC CCACATTGCA
    GCCAAGACAG TATTCCATTT GGCCCATCGT CATGGTGATA TCCTGCGGGA GGGCTGGAAG
    AATATCATGG AAGCCATGCT GCAGCTCTTC CGAGCCCAAC TGCTTCCCAA AGCTATGGTG
    GAGGTAGAAG ATTTTGTGGA TCCCAATGGC AAGATCTCTC TACAGAGGGA GGAGACACCA
    TCAAACCGAG GAGAGTCGAC AGTGCTAAGC TTTGTGAGCT GGCTAACACT AAGTGGTACT
    GAGCAGTCTA GTGTGCGGGG CCCATCCACT GAGAACCAAG AGGCCAAAAG AGCGGCCTTG
    GACTGCATCA AGCATTGTGA CCCAGAAAAG ATGATCACAG AAAGCAAGTT CCTTCAGCTG
    GAGTCACTGC AGGAGCTCAT GAAGGCTCTG GTCTCAGTGA CGCCAGATGA AGAGACATAT
    GATGAGGAGG ATGCTGCTTT CTGCCTAGAG ATGCTGCTGA GGATTGTGCT GGAGAACAGG
    GACCGTGTGG GTTGTGTGTG GCAGACTGTC CGAGACCATC TATATCACCT GTGTGTCCAG
    GCACAGGATT TCTGCTTCCT TGTGGAGCGG GCAGTGGTGG GGCTGCTCCG CTTGGCCATT
    CGACTACTCC GAAGAGAAGA GATCAGTGGC CAGGTGCTGC TCTCCCTGCG TATTTTGCTA
    CTAATGAAGC CCAGCGTGCT GTCCCGAGTC AGTCACCAGG TAGCCTATGG GCTCCATGAA
    CTTCTTAAGA CCAACGCAGC CAACATCCAC TCAGGTGACG ACTGGGCCAC TCTCTTCACA
    CTGCTGGAAT GCATTGGCTC AGGTGTAAAG CCTCCAGCTG CCCTGCAGGC CACAGCCAGG
    GCTGAAGCAC CTGATGCCGG GGCCCAATCA GACAGCGAAC TCCCATCCTA CCATCAGAAT
    GATGTGAGCC TGGACCGAGG ATACACTTCT GACTCGGAGG TCTATGCTGA CCATTGCAGG
    CCTGGCAAGA TCCACCGATC AGCCACTGAT GCTGATGTAG TCAACAGCGG CTGGTTAGTG
    GTGGGGAAGG ATGACATTGA TAACTCCAAG CCAGGTCCTG GGCCCAGCCG GCTAGGCCCT
    TCACCCCTGG TCAATCAGTA CAGCCTAACA GTGGGGCTGG ACCTCGGGCC ACACGACACT
    AAGTCCCTGC TTAAATGTGT AGAATCTCTG TCCTTCATTG TGCGTGATGC TGCCCACATT
    ACACCTGACA ACTTTGAGCT CTGCGTGAAG ACTCTCCGTA TCTTTGTGGA GGCCAGTCTG
    AATGGCGGGT GCAAGTCCCA GGAGAAACGT GGCAAGAGTC ACAAATATGA CAGCAAAGGA
    AACCGCTTCA AGAAGAAATC CAAGGAAGGT TCAGTGCTTC GGCGGCCTCG AACCTCTGGC
    CAACATGCCA CTCGGGGTGG GCATAGTGAC GATGATGAGG ATGAAGGGGT GCCTGCCAGC
    TACCATACGG TGTCTTTACA GGTCAGTCAG GACTTGTTAG ATCTGATGCA CACCCTGCAC
    ACGCGAGCAG CCTCTATCTA CAGCTCTTGG GCGGAGGAAC AGCGCCACCT AGAGACAGGT
    GGCCGGAAGA TAGAAGCGGA TTCACGTACC CTCTGGGCCC ACTGCTGGTG TCCCTTACTG
    CAGGGCATTG CCTGCTTGTG CTGTGATGCC CGGCGCCAGG TGCGGATGCA AGCACTGACT
    TATCTGCAAC GAGCACTACT GGTACATGAT CTGCAGAAGC TAGATGCCCT GGAGTGGGAG
    TCCTGTTTTA ACAAGGTGCT GTTTCCTCTA CTGACCAAAC TCTTGGAGAA CATCAGCCCT
    GCAGATGTGG GTGGGATGGA GGAGACTCGA ATGAGGGCTT CCACACTGCT GTCTAAGGTC
    TTCCTGCAGC ACCTGTCTCC ACTGCTGTCA CTCTCTACCT TTGCGGCCCT CTGGCTGACC
    ATCCTGGACT TCATGGACAA GTATATGCAT GCAGGATCCA GTGACTTACT GTCAGAGGCC
    ATCCCTGAGT CTCTGAAGAA CATGCTCCTG GTGATGGACA CAGCAGAGAT TTTCCACAAT
    GCAGATGCCC GAGGAGGCAG CCCCTCAGCC CTCTGGGAGA TCACCTGGGA GCGCATTGAC
    TGTTTTCTGC CCCACCTACG AGATGAGCTC TTCAAGCAGA CTGTCATCCA GGACCCCATG
    CCACTGGAGC CACATGCCCA AAAAACTCTG GCCTCAGCCC ACATGACTCC TGCTGCTGGA
    GACACAAGGA CACCTGGCCA TCCACCTCCC CCAGAGATGC CTTCTGAGCT GGGGGCCTGT
    GACTTCGAGA AGCCTGAGGG CCCTCGACCT GCCAACAGCA GCTCTCCTGG ATCACCAGTG
    GCATCTAGCC CCAACAGACT GAGCCCTACT TCAGATGGGC CTCCTCCTCT GGCTCAGCCT
    CCACTGATCC TGCAGCCCTT GGCCTCCCCA TTGCAGGTGG GCGTACCACC CATGACTCTA
    CCAATCATCC TCAACCCTGC TCTCATTGAG GCCACTTCAC CAGTGCCCCT CCTGGCCACA
    CCCCGCCCCA CAGACCCAAT GCCCACCTCC GAGGTCAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27948
    Clone ID Related Accession (Same CDS sequence) XM_005637658.3 , XM_005637658.2
    Accession Version XM_005637658.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5682bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726109.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005637658.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
    AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA
    CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT
    CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGA TTTCTCCATG TCTCAACAAA
    CTGGAGCTAA GTAGTGGGGA GCAGACCAAA GCCCTGAACC AGTTAGAGAG GGTACTACTC
    TTTAAGAACC TCAAGCTGAA AATGAGAGCA GGAGGCATGA GTGATTCATC CAAGTGGAAG
    AAACAGAAGA GATCCCCCCG GCCCCTGCGC CATATGACCA AAGTAACATC AGGTTCAGAG
    TTGCCCACCT CCAATGGAAC CACCTTATCC TCTAACCTCA CTGGGGGCAT GCCCTTCATT
    GATGTGCCCA CTCCCATCTC CTCTGCAAGT TCAGAAGCTG CCTCAACGGT GGTCAGCCCC
    TCTACAGACA GTGGCCTGGA GTTCTCCTCT CAGACCACCT CCAAGGAGGA CCTCACTGAC
    CTGGAGCAAC CTGGCTCTCC AGGGTATAGC ACAGCTACAG AGCCTGGTAG CAGTGAGCTA
    GGGGTTCCTG AGCAGCCTGA CCCGCAGGAA GGAGTCCATG TGGAAAAGGC CCAGTCAGCA
    TCTGTGGAAT CCATCCCCGA AGTGTTAGAG GAGTGCACGT CTCCTGCTGA CCACTCTGAC
    TCTGCCTCTG TCCATGACAT GGATTACGTC AATCCCCGGG GTGTGCGCTT TACACAGTCC
    TCCCAGAAAG AAGGTACAGC TTTGGTTCCC TATGGTCTTC CCTGCATCCG TGAGCTCTTC
    CGCTTCCTCA TCTCCCTCAC CAATCCACAT GACCGCCACA ATTCAGAAGT TATGATCCAC
    ATGGGACTGC ATTTGCTGAC AGTGGCTCTT GAATCTGCCC CTGTAGCCCA GTGCCAAACC
    CTTTTGGGCC TCATCAAAGA TGAGATGTGC CGCCACTTAC TCCAGCTACT CAGTGTAGAG
    CGACTCAACC TTTATGCTGC TTCCCTGCGG GTATGCTTCC TGCTTTTTGA GAGCATGAGG
    GAACATCTCA AGTTCCAATT GGAGATGTAC ATCAAAAAGC TCATGGAGAT CATCACTGTG
    GAGAACCCCA AAATGCCTTA TGAGATGAAA GAGATGGCAC TGGAGGCCAT TGTGCAGCTC
    TGGCACATCC CCAGTTTTGT CACTGAGCTC TATATCAACT ATGATTGTGA CTACTACTGT
    TCAAACCTCT TTGAAGACCT CACCAAGCTG CTGTCCAAGA ATGCCTTCCC TGTGTCTGGT
    CAGCTCTATA CAACACACCT CCTGTCTCTT GATGCCCTGT TGACAGTGAT TGACAGCACT
    GAGGCCCATT GCCAGGCCAA AGTCCTCAAC AGTCTTACCC AGCAAGAGAA GAAAGAAGCA
    GCCAGATCTG GCTATGAGGC GGTGGATGGT ACCCGAGAAT CCAGCAATAG TGAGAGAGCA
    GCCAGCGATG GGAAACCTGT AGGCATAGCC GCAGACATCC CAGGTCTACA TCTGCCAGGT
    GGAGGGCGGC TGCCAGCAGA ACATGGGAAG CCAGGATGCA GTGATCTGGA GGAAGCTGCT
    GACTCTGGGG CTGACAAAAA GTTTACCCGG AAGCCACCTC GATTTTCCTG TCTCCTGCCA
    GATCCACGGG AACTGATTGA AATTAAAAAC AAAAAGAAGC TGCTAATCAC TGGCACAGAG
    CAGTTCAACC AGAAACCAAA GAAGGGAATC CAGTTTCTGC AGGAGAAAGG CCTCCTCACC
    ATCCCAATGG ACAACACAGA GGTAGCCCAG TGGCTCCGAG AGAACCCTCG GCTGGACAAG
    AAAATGATTG GAGAGTTTGT GAGTGACCGC AAAAACATTG ACCTGTTGGA GAGCTTTGTG
    AGCACCTTCA GCTTTCAGGG TCTGCGGCTG GATGAAGCTC TTCGTCTCTA CCTGGAAGCC
    TTTCGCTTAC CTGGGGAAGC ACCAGTCATC CAGAGGTTGC TGGAGGCATT CACAGAGCAT
    TGGAGGAATT GTAATGGCTC CCCATTTGCC AATAGCGATG CCTGCTTTGC TCTGGCCTAT
    GCTGTCATCT TGCTTAATAC TGACCAGCAC AACCACAACG TTCGCAAACA GAATGCACCC
    ATGACTCTAG AGGAGTTTCG CAAAAACCTA AAAGGTGTGA ATGGAGGCAA GGACTTTGAG
    CAAGACATCC TGGAGGACAT GTACCATGCC ATCAAGAATG AGGAAATTGT GATGCCTGAA
    GAGCAGACAG GCTTGGTTCG GGAGAACTAT GTGTGGAATG TGCTACTTCA TCGAGGTGCC
    ACCCCAGAGG GCATATTCCT TCGTGTGCCT GCTGGCAGCT ATGATCTTGA CCTCTTCACC
    ATGACGTGGG GCCCCACTAT TGCTGCCCTT TCTTATGTCT TTGACAAAAG CCTTGAGGAA
    ACAATTATCC AGAAAGCCAT CTCAGGCTTC AGGAAGTGCG CCATGATCTC CGCCCACTAT
    GGCCTCAGCG ATGTGTTTGA CAATCTCATC ATATCTCTGT GCAAATTCAC AGCTCTTAGC
    AGTGAGTCTA TTGAGAATCT GCCCAGTGTG TTTGGAAGCA ACCCCAAAGC CCACATTGCA
    GCCAAGACAG TATTCCATTT GGCCCATCGT CATGGTGATA TCCTGCGGGA GGGCTGGAAG
    AATATCATGG AAGCCATGCT GCAGCTCTTC CGAGCCCAAC TGCTTCCCAA AGCTATGGTG
    GAGGTAGAAG ATTTTGTGGA TCCCAATGGC AAGATCTCTC TACAGAGGGA GGAGACACCA
    TCAAACCGAG GAGAGTCGAC AGTGCTAAGC TTTGTGAGCT GGCTAACACT AAGTGGTACT
    GAGCAGTCTA GTGTGCGGGG CCCATCCACT GAGAACCAAG AGGCCAAAAG AGCGGCCTTG
    GACTGCATCA AGCATTGTGA CCCAGAAAAG ATGATCACAG AAAGCAAGTT CCTTCAGCTG
    GAGTCACTGC AGGAGCTCAT GAAGGCTCTG GTCTCAGTGA CGCCAGATGA AGAGACATAT
    GATGAGGAGG ATGCTGCTTT CTGCCTAGAG ATGCTGCTGA GGATTGTGCT GGAGAACAGG
    GACCGTGTGG GTTGTGTGTG GCAGACTGTC CGAGACCATC TATATCACCT GTGTGTCCAG
    GCACAGGATT TCTGCTTCCT TGTGGAGCGG GCAGTGGTGG GGCTGCTCCG CTTGGCCATT
    CGACTACTCC GAAGAGAAGA GATCAGTGGC CAGGTGCTGC TCTCCCTGCG TATTTTGCTA
    CTAATGAAGC CCAGCGTGCT GTCCCGAGTC AGTCACCAGG TAGCCTATGG GCTCCATGAA
    CTTCTTAAGA CCAACGCAGC CAACATCCAC TCAGGTGACG ACTGGGCCAC TCTCTTCACA
    CTGCTGGAAT GCATTGGCTC AGGTGTAAAG CCTCCAGCTG CCCTGCAGGC CACAGCCAGG
    GCTGAAGCAC CTGATGCCGG GGCCCAATCA GACAGCGAAC TCCCATCCTA CCATCAGAAT
    GATGTGAGCC TGGACCGAGG ATACACTTCT GACTCGGAGG TCTATGCTGA CCATTGCAGG
    CCTGGCAAGA TCCACCGATC AGCCACTGAT GCTGATGTAG TCAACAGCGG CTGGTTAGTG
    GTGGGGAAGG ATGACATTGA TAACTCCAAG CCAGGTCCTG GGCCCAGCCG GCTAGGCCCT
    TCACCCCTGG TCAATCAGTA CAGCCTAACA GTGGGGCTGG ACCTCGGGCC ACACGACACT
    AAGTCCCTGC TTAAATGTGT AGAATCTCTG TCCTTCATTG TGCGTGATGC TGCCCACATT
    ACACCTGACA ACTTTGAGCT CTGCGTGAAG ACTCTCCGTA TCTTTGTGGA GGCCAGTCTG
    AATGGCGGGT GCAAGTCCCA GGAGAAACGT GGCAAGAGTC ACAAATATGA CAGCAAAGGA
    AACCGCTTCA AGAAGAAATC CAAGGAAGGT TCAGTGCTTC GGCGGCCTCG AACCTCTGGC
    CAACATGCCA CTCGGGGTGG GCATAGTGAC GATGATGAGG ATGAAGGGGT GCCTGCCAGC
    TACCATACGG TGTCTTTACA GGTCAGTCAG GACTTGTTAG ATCTGATGCA CACCCTGCAC
    ACGCGAGCAG CCTCTATCTA CAGCTCTTGG GCGGAGGAAC AGCGCCACCT AGAGACAGGT
    GGCCGGAAGA TAGAAGCGGA TTCACGTACC CTCTGGGCCC ACTGCTGGTG TCCCTTACTG
    CAGGGCATTG CCTGCTTGTG CTGTGATGCC CGGCGCCAGG TGCGGATGCA AGCACTGACT
    TATCTGCAAC GAGCACTACT GGTACATGAT CTGCAGAAGC TAGATGCCCT GGAGTGGGAG
    TCCTGTTTTA ACAAGGTGCT GTTTCCTCTA CTGACCAAAC TCTTGGAGAA CATCAGCCCT
    GCAGATGTGG GTGGGATGGA GGAGACTCGA ATGAGGGCTT CCACACTGCT GTCTAAGGTC
    TTCCTGCAGC ACCTGTCTCC ACTGCTGTCA CTCTCTACCT TTGCGGCCCT CTGGCTGACC
    ATCCTGGACT TCATGGACAA GTATATGCAT GCAGGATCCA GTGACTTACT GTCAGAGGCC
    ATCCCTGAGT CTCTGAAGAA CATGCTCCTG GTGATGGACA CAGCAGAGAT TTTCCACAAT
    GCAGATGCCC GAGGAGGCAG CCCCTCAGCC CTCTGGGAGA TCACCTGGGA GCGCATTGAC
    TGTTTTCTGC CCCACCTACG AGATGAGCTC TTCAAGCAGA CTGTCATCCA GGACCCCATG
    CCACTGGAGC CACATGCCCA AAAAACTCTG GCCTCAGCCC ACATGACTCC TGCTGCTGGA
    GACACAAGGA CACCTGGCCA TCCACCTCCC CCAGAGATGC CTTCTGAGCT GGGGGCCTGT
    GACTTCGAGA AGCCTGAGGG CCCTCGACCT GCCAACAGCA GCTCTCCTGG ATCACCAGTG
    GCATCTAGCC CCAACAGACT GAGCCCTACT TCAGATGGGC CTCCTCCTCT GGCTCAGCCT
    CCACTGATCC TGCAGCCCTT GGCCTCCCCA TTGCAGGTGG GCGTACCACC CATGACTCTA
    CCAATCATCC TCAACCCTGC TCTCATTGAG GCCACTTCAC CAGTGCCCCT CCTGGCCACA
    CCCCGCCCCA CAGACCCAAT GCCCACCTCC GAGGTCAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005637715.1
    CDS251..5932
    Translation

    Target ORF information:

    RefSeq Version XM_005637658.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005637658.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
    AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA
    CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT
    CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGA TTTCTCCATG TCTCAACAAA
    CTGGAGCTAA GTAGTGGGGA GCAGACCAAA GCCCTGAACC AGTTAGAGAG GGTACTACTC
    TTTAAGAACC TCAAGCTGAA AATGAGAGCA GGAGGCATGA GTGATTCATC CAAGTGGAAG
    AAACAGAAGA GATCCCCCCG GCCCCTGCGC CATATGACCA AAGTAACATC AGGTTCAGAG
    TTGCCCACCT CCAATGGAAC CACCTTATCC TCTAACCTCA CTGGGGGCAT GCCCTTCATT
    GATGTGCCCA CTCCCATCTC CTCTGCAAGT TCAGAAGCTG CCTCAACGGT GGTCAGCCCC
    TCTACAGACA GTGGCCTGGA GTTCTCCTCT CAGACCACCT CCAAGGAGGA CCTCACTGAC
    CTGGAGCAAC CTGGCTCTCC AGGGTATAGC ACAGCTACAG AGCCTGGTAG CAGTGAGCTA
    GGGGTTCCTG AGCAGCCTGA CCCGCAGGAA GGAGTCCATG TGGAAAAGGC CCAGTCAGCA
    TCTGTGGAAT CCATCCCCGA AGTGTTAGAG GAGTGCACGT CTCCTGCTGA CCACTCTGAC
    TCTGCCTCTG TCCATGACAT GGATTACGTC AATCCCCGGG GTGTGCGCTT TACACAGTCC
    TCCCAGAAAG AAGGTACAGC TTTGGTTCCC TATGGTCTTC CCTGCATCCG TGAGCTCTTC
    CGCTTCCTCA TCTCCCTCAC CAATCCACAT GACCGCCACA ATTCAGAAGT TATGATCCAC
    ATGGGACTGC ATTTGCTGAC AGTGGCTCTT GAATCTGCCC CTGTAGCCCA GTGCCAAACC
    CTTTTGGGCC TCATCAAAGA TGAGATGTGC CGCCACTTAC TCCAGCTACT CAGTGTAGAG
    CGACTCAACC TTTATGCTGC TTCCCTGCGG GTATGCTTCC TGCTTTTTGA GAGCATGAGG
    GAACATCTCA AGTTCCAATT GGAGATGTAC ATCAAAAAGC TCATGGAGAT CATCACTGTG
    GAGAACCCCA AAATGCCTTA TGAGATGAAA GAGATGGCAC TGGAGGCCAT TGTGCAGCTC
    TGGCACATCC CCAGTTTTGT CACTGAGCTC TATATCAACT ATGATTGTGA CTACTACTGT
    TCAAACCTCT TTGAAGACCT CACCAAGCTG CTGTCCAAGA ATGCCTTCCC TGTGTCTGGT
    CAGCTCTATA CAACACACCT CCTGTCTCTT GATGCCCTGT TGACAGTGAT TGACAGCACT
    GAGGCCCATT GCCAGGCCAA AGTCCTCAAC AGTCTTACCC AGCAAGAGAA GAAAGAAGCA
    GCCAGATCTG GCTATGAGGC GGTGGATGGT ACCCGAGAAT CCAGCAATAG TGAGAGAGCA
    GCCAGCGATG GGAAACCTGT AGGCATAGCC GCAGACATCC CAGGTCTACA TCTGCCAGGT
    GGAGGGCGGC TGCCAGCAGA ACATGGGAAG CCAGGATGCA GTGATCTGGA GGAAGCTGCT
    GACTCTGGGG CTGACAAAAA GTTTACCCGG AAGCCACCTC GATTTTCCTG TCTCCTGCCA
    GATCCACGGG AACTGATTGA AATTAAAAAC AAAAAGAAGC TGCTAATCAC TGGCACAGAG
    CAGTTCAACC AGAAACCAAA GAAGGGAATC CAGTTTCTGC AGGAGAAAGG CCTCCTCACC
    ATCCCAATGG ACAACACAGA GGTAGCCCAG TGGCTCCGAG AGAACCCTCG GCTGGACAAG
    AAAATGATTG GAGAGTTTGT GAGTGACCGC AAAAACATTG ACCTGTTGGA GAGCTTTGTG
    AGCACCTTCA GCTTTCAGGG TCTGCGGCTG GATGAAGCTC TTCGTCTCTA CCTGGAAGCC
    TTTCGCTTAC CTGGGGAAGC ACCAGTCATC CAGAGGTTGC TGGAGGCATT CACAGAGCAT
    TGGAGGAATT GTAATGGCTC CCCATTTGCC AATAGCGATG CCTGCTTTGC TCTGGCCTAT
    GCTGTCATCT TGCTTAATAC TGACCAGCAC AACCACAACG TTCGCAAACA GAATGCACCC
    ATGACTCTAG AGGAGTTTCG CAAAAACCTA AAAGGTGTGA ATGGAGGCAA GGACTTTGAG
    CAAGACATCC TGGAGGACAT GTACCATGCC ATCAAGAATG AGGAAATTGT GATGCCTGAA
    GAGCAGACAG GCTTGGTTCG GGAGAACTAT GTGTGGAATG TGCTACTTCA TCGAGGTGCC
    ACCCCAGAGG GCATATTCCT TCGTGTGCCT GCTGGCAGCT ATGATCTTGA CCTCTTCACC
    ATGACGTGGG GCCCCACTAT TGCTGCCCTT TCTTATGTCT TTGACAAAAG CCTTGAGGAA
    ACAATTATCC AGAAAGCCAT CTCAGGCTTC AGGAAGTGCG CCATGATCTC CGCCCACTAT
    GGCCTCAGCG ATGTGTTTGA CAATCTCATC ATATCTCTGT GCAAATTCAC AGCTCTTAGC
    AGTGAGTCTA TTGAGAATCT GCCCAGTGTG TTTGGAAGCA ACCCCAAAGC CCACATTGCA
    GCCAAGACAG TATTCCATTT GGCCCATCGT CATGGTGATA TCCTGCGGGA GGGCTGGAAG
    AATATCATGG AAGCCATGCT GCAGCTCTTC CGAGCCCAAC TGCTTCCCAA AGCTATGGTG
    GAGGTAGAAG ATTTTGTGGA TCCCAATGGC AAGATCTCTC TACAGAGGGA GGAGACACCA
    TCAAACCGAG GAGAGTCGAC AGTGCTAAGC TTTGTGAGCT GGCTAACACT AAGTGGTACT
    GAGCAGTCTA GTGTGCGGGG CCCATCCACT GAGAACCAAG AGGCCAAAAG AGCGGCCTTG
    GACTGCATCA AGCATTGTGA CCCAGAAAAG ATGATCACAG AAAGCAAGTT CCTTCAGCTG
    GAGTCACTGC AGGAGCTCAT GAAGGCTCTG GTCTCAGTGA CGCCAGATGA AGAGACATAT
    GATGAGGAGG ATGCTGCTTT CTGCCTAGAG ATGCTGCTGA GGATTGTGCT GGAGAACAGG
    GACCGTGTGG GTTGTGTGTG GCAGACTGTC CGAGACCATC TATATCACCT GTGTGTCCAG
    GCACAGGATT TCTGCTTCCT TGTGGAGCGG GCAGTGGTGG GGCTGCTCCG CTTGGCCATT
    CGACTACTCC GAAGAGAAGA GATCAGTGGC CAGGTGCTGC TCTCCCTGCG TATTTTGCTA
    CTAATGAAGC CCAGCGTGCT GTCCCGAGTC AGTCACCAGG TAGCCTATGG GCTCCATGAA
    CTTCTTAAGA CCAACGCAGC CAACATCCAC TCAGGTGACG ACTGGGCCAC TCTCTTCACA
    CTGCTGGAAT GCATTGGCTC AGGTGTAAAG CCTCCAGCTG CCCTGCAGGC CACAGCCAGG
    GCTGAAGCAC CTGATGCCGG GGCCCAATCA GACAGCGAAC TCCCATCCTA CCATCAGAAT
    GATGTGAGCC TGGACCGAGG ATACACTTCT GACTCGGAGG TCTATGCTGA CCATTGCAGG
    CCTGGCAAGA TCCACCGATC AGCCACTGAT GCTGATGTAG TCAACAGCGG CTGGTTAGTG
    GTGGGGAAGG ATGACATTGA TAACTCCAAG CCAGGTCCTG GGCCCAGCCG GCTAGGCCCT
    TCACCCCTGG TCAATCAGTA CAGCCTAACA GTGGGGCTGG ACCTCGGGCC ACACGACACT
    AAGTCCCTGC TTAAATGTGT AGAATCTCTG TCCTTCATTG TGCGTGATGC TGCCCACATT
    ACACCTGACA ACTTTGAGCT CTGCGTGAAG ACTCTCCGTA TCTTTGTGGA GGCCAGTCTG
    AATGGCGGGT GCAAGTCCCA GGAGAAACGT GGCAAGAGTC ACAAATATGA CAGCAAAGGA
    AACCGCTTCA AGAAGAAATC CAAGGAAGGT TCAGTGCTTC GGCGGCCTCG AACCTCTGGC
    CAACATGCCA CTCGGGGTGG GCATAGTGAC GATGATGAGG ATGAAGGGGT GCCTGCCAGC
    TACCATACGG TGTCTTTACA GGTCAGTCAG GACTTGTTAG ATCTGATGCA CACCCTGCAC
    ACGCGAGCAG CCTCTATCTA CAGCTCTTGG GCGGAGGAAC AGCGCCACCT AGAGACAGGT
    GGCCGGAAGA TAGAAGCGGA TTCACGTACC CTCTGGGCCC ACTGCTGGTG TCCCTTACTG
    CAGGGCATTG CCTGCTTGTG CTGTGATGCC CGGCGCCAGG TGCGGATGCA AGCACTGACT
    TATCTGCAAC GAGCACTACT GGTACATGAT CTGCAGAAGC TAGATGCCCT GGAGTGGGAG
    TCCTGTTTTA ACAAGGTGCT GTTTCCTCTA CTGACCAAAC TCTTGGAGAA CATCAGCCCT
    GCAGATGTGG GTGGGATGGA GGAGACTCGA ATGAGGGCTT CCACACTGCT GTCTAAGGTC
    TTCCTGCAGC ACCTGTCTCC ACTGCTGTCA CTCTCTACCT TTGCGGCCCT CTGGCTGACC
    ATCCTGGACT TCATGGACAA GTATATGCAT GCAGGATCCA GTGACTTACT GTCAGAGGCC
    ATCCCTGAGT CTCTGAAGAA CATGCTCCTG GTGATGGACA CAGCAGAGAT TTTCCACAAT
    GCAGATGCCC GAGGAGGCAG CCCCTCAGCC CTCTGGGAGA TCACCTGGGA GCGCATTGAC
    TGTTTTCTGC CCCACCTACG AGATGAGCTC TTCAAGCAGA CTGTCATCCA GGACCCCATG
    CCACTGGAGC CACATGCCCA AAAAACTCTG GCCTCAGCCC ACATGACTCC TGCTGCTGGA
    GACACAAGGA CACCTGGCCA TCCACCTCCC CCAGAGATGC CTTCTGAGCT GGGGGCCTGT
    GACTTCGAGA AGCCTGAGGG CCCTCGACCT GCCAACAGCA GCTCTCCTGG ATCACCAGTG
    GCATCTAGCC CCAACAGACT GAGCCCTACT TCAGATGGGC CTCCTCCTCT GGCTCAGCCT
    CCACTGATCC TGCAGCCCTT GGCCTCCCCA TTGCAGGTGG GCGTACCACC CATGACTCTA
    CCAATCATCC TCAACCCTGC TCTCATTGAG GCCACTTCAC CAGTGCCCCT CCTGGCCACA
    CCCCGCCCCA CAGACCCAAT GCCCACCTCC GAGGTCAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27949
    Clone ID Related Accession (Same CDS sequence) XM_005637659.2 , XM_005637659.3
    Accession Version XM_005637659.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5673bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726109.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005637659.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
    AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA
    CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT
    CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGA TTTCTCCATG TCTCAACAAA
    CTGGAGCTAA GTAGTGGGGA GCAGACCAAA GCCCTGAACC AGTTAGAGAG GGTACTACTC
    TTTAAGAACC TCAAGCTGAA AATGAGAGCA GGAGGCATGA GTGATTCATC CAAGTGGAAG
    AAACAGAAGA GATCCCCCCG GCCCCTGCGC CATATGACCA AAGTAACATC AGGTTCAGAG
    TTGCCCACCT CCAATGGAAC CACCTTATCC TCTAACCTCA CTGGGGGCAT GCCCTTCATT
    GATGTGCCCA CTCCCATCTC CTCTGCAAGT TCAGAAGCTG CCTCAACGGT GGTCAGCCCC
    TCTACAGACA GTGGCCTGGA GTTCTCCTCT CAGACCACCT CCAAGGAGGA CCTCACTGAC
    CTGGAGCAAC CTGGCTCTCC AGGGTATAGC ACAGCTACAG AGCCTGGTAG CAGTGAGCTA
    GGGGTTCCTG AGCAGCCTGA CCCGCAGCAG GAAGGAGTCC ATGTGGAAAA GGCCCAGTCA
    GCATCTGTGG AATCCATCCC CGAAGTGTTA GAGGAGTGCA CGTCTCCTGC TGACCACTCT
    GACTCTGCCT CTGTCCATGA CATGGATTAC GTCAATCCCC GGGGTGTGCG CTTTACACAG
    TCCTCCCAGA AAGAAGGTAC AGCTTTGGTT CCCTATGGTC TTCCCTGCAT CCGTGAGCTC
    TTCCGCTTCC TCATCTCCCT CACCAATCCA CATGACCGCC ACAATTCAGA AGTTATGATC
    CACATGGGAC TGCATTTGCT GACAGTGGCT CTTGAATCTG CCCCTGTAGC CCAGTGCCAA
    ACCCTTTTGG GCCTCATCAA AGATGAGATG TGCCGCCACT TACTCCAGCT ACTCAGTGTA
    GAGCGACTCA ACCTTTATGC TGCTTCCCTG CGGGTATGCT TCCTGCTTTT TGAGAGCATG
    AGGGAACATC TCAAGTTCCA ATTGGAGATG TACATCAAAA AGCTCATGGA GATCATCACT
    GTGGAGAACC CCAAAATGCC TTATGAGATG AAAGAGATGG CACTGGAGGC CATTGTGCAG
    CTCTGGCACA TCCCCAGTTT TGTCACTGAG CTCTATATCA ACTATGATTG TGACTACTAC
    TGTTCAAACC TCTTTGAAGA CCTCACCAAG CTGCTGTCCA AGAATGCCTT CCCTGTGTCT
    GGTCAGCTCT ATACAACACA CCTCCTGTCT CTTGATGCCC TGTTGACAGT GATTGACAGC
    ACTGAGGCCC ATTGCCAGGC CAAAGTCCTC AACAGTCTTA CCCAGCAAGA GAAGAAAGAA
    GCAGCCAGAT CTGGCTATGA GGCGGTGGAT GGTACCCGAG AATCCAGCAA TAGTGAGAGA
    GCAGCCAGCG ATGGGAAACC TGTAGGCATA GCCGCAGACA TCCCAGGTCT ACATCTGCCA
    GGTGGAGGGC GGCTGCCAGC AGAACATGGG AAGCCAGGAT GCAGTGATCT GGAGGAAGCT
    GCTGACTCTG GGGCTGACAA AAAGTTTACC CGGAAGCCAC CTCGATTTTC CTGTCTCCTG
    CCAGATCCAC GGGAACTGAT TGAAATTAAA AACAAAAAGA AGCTGCTAAT CACTGGCACA
    GAGCAGTTCA ACCAGAAACC AAAGAAGGGA ATCCAGTTTC TGCAGGAGAA AGGCCTCCTC
    ACCATCCCAA TGGACAACAC AGAGGTAGCC CAGTGGCTCC GAGAGAACCC TCGGCTGGAC
    AAGAAAATGA TTGGAGAGTT TGTGAGTGAC CGCAAAAACA TTGACCTGTT GGAGAGCTTT
    GTGAGCACCT TCAGCTTTCA GGGTCTGCGG CTGGATGAAG CTCTTCGTCT CTACCTGGAA
    GCCTTTCGCT TACCTGGGGA AGCACCAGTC ATCCAGAGGT TGCTGGAGGC ATTCACAGAG
    CATTGGAGGA ATTGTAATGG CTCCCCATTT GCCAATAGCG ATGCCTGCTT TGCTCTGGCC
    TATGCTGTCA TCTTGCTTAA TACTGACCAG CACAACCACA ACGTTCGCAA ACAGAATGCA
    CCCATGACTC TAGAGGAGTT TCGCAAAAAC CTAAAAGGTG TGAATGGAGG CAAGGACTTT
    GAGCAAGACA TCCTGGAGGA CATGTACCAT GCCATCAAGA ATGAGGAAAT TGTGATGCCT
    GAAGAGCAGA CAGGCTTGGT TCGGGAGAAC TATGTGTGGA ATGTGCTACT TCATCGAGGT
    GCCACCCCAG AGGGCATATT CCTTCGTGTG CCTGCTGGCA GCTATGATCT TGACCTCTTC
    ACCATGACGT GGGGCCCCAC TATTGCTGCC CTTTCTTATG TCTTTGACAA AAGCCTTGAG
    GAAACAATTA TCCAGAAAGC CATCTCAGGC TTCAGGAAGT GCGCCATGAT CTCCGCCCAC
    TATGGCCTCA GCGATGTGTT TGACAATCTC ATCATATCTC TGTGCAAATT CACAGCTCTT
    AGCAGTGAGT CTATTGAGAA TCTGCCCAGT GTGTTTGGAA GCAACCCCAA AGCCCACATT
    GCAGCCAAGA CAGTATTCCA TTTGGCCCAT CGTCATGGTG ATATCCTGCG GGAGGGCTGG
    AAGAATATCA TGGAAGCCAT GCTGCAGCTC TTCCGAGCCC AACTGCTTCC CAAAGCTATG
    GTGGAGGTAG AAGATTTTGT GGATCCCAAT GGCAAGATCT CTCTACAGAG GGAGGAGACA
    CCATCAAACC GAGGAGAGTC GACAGTGCTA AGCTTTGTGA GCTGGCTAAC ACTAAGTGGT
    ACTGAGCAGT CTAGTGTGCG GGGCCCATCC ACTGAGAACC AAGAGGCCAA AAGAGCGGCC
    TTGGACTGCA TCAAGCATTG TGACCCAGAA AAGATGATCA CAGAAAGCAA GTTCCTTCAG
    CTGGAGTCAC TGCAGGAGCT CATGAAGGCT CTGGTCTCAG TGACGCCAGA TGAAGAGACA
    TATGATGAGG AGGATGCTGC TTTCTGCCTA GAGATGCTGC TGAGGATTGT GCTGGAGAAC
    AGGGACCGTG TGGGTTGTGT GTGGCAGACT GTCCGAGACC ATCTATATCA CCTGTGTGTC
    CAGGCACAGG ATTTCTGCTT CCTTGTGGAG CGGGCAGTGG TGGGGCTGCT CCGCTTGGCC
    ATTCGACTAC TCCGAAGAGA AGAGATCAGT GGCCAGGTGC TGCTCTCCCT GCGTATTTTG
    CTACTAATGA AGCCCAGCGT GCTGTCCCGA GTCAGTCACC AGGTAGCCTA TGGGCTCCAT
    GAACTTCTTA AGACCAACGC AGCCAACATC CACTCAGGTG ACGACTGGGC CACTCTCTTC
    ACACTGCTGG AATGCATTGG CTCAGGTGTA AAGCCTCCAG CTGCCCTGCA GGCCACAGCC
    AGGGCTGAAG CACCTGATGC CGGGGCCCAA TCAGACAGCG AACTCCCATC CTACCATCAG
    AATGATGTGA GCCTGGACCG AGGATACACT TCTGACTCGG AGGTCTATGC TGACCATTGC
    AGGCCTGGCA AGATCCACCG ATCAGCCACT GATGCTGATG TAGTCAACAG CGGCTGGTTA
    GTGGTGGGGA AGGATGACAT TGATAACTCC AAGCCAGGTC CTGGGCCCAG CCGGCTAGGC
    CCTTCACCCC TGGTCAATCA GTACAGCCTA ACAGTGGGGC TGGACCTCGG GCCACACGAC
    ACTAAGTCCC TGCTTAAATG TGTAGAATCT CTGTCCTTCA TTGTGCGTGA TGCTGCCCAC
    ATTACACCTG ACAACTTTGA GCTCTGCGTG AAGACTCTCC GTATCTTTGT GGAGGCCAGT
    CTGAATGGCG GGTGCAAGTC CCAGGAGAAA CGTGGCAAGA GTCACAAATA TGACAGCAAA
    GGAAACCGCT TCAAGAAGAA ATCCAAGGAA GGTTCAGTGC TTCGGCGGCC TCGAACCTCT
    GGCCAACATG CCACTCGGGG TGGGCATAGT GACGATGATG AGGATGAAGG GGTGCCTGCC
    AGCTACCATA CGGTGTCTTT ACAGTTGTTA GATCTGATGC ACACCCTGCA CACGCGAGCA
    GCCTCTATCT ACAGCTCTTG GGCGGAGGAA CAGCGCCACC TAGAGACAGG TGGCCGGAAG
    ATAGAAGCGG ATTCACGTAC CCTCTGGGCC CACTGCTGGT GTCCCTTACT GCAGGGCATT
    GCCTGCTTGT GCTGTGATGC CCGGCGCCAG GTGCGGATGC AAGCACTGAC TTATCTGCAA
    CGAGCACTAC TGGTACATGA TCTGCAGAAG CTAGATGCCC TGGAGTGGGA GTCCTGTTTT
    AACAAGGTGC TGTTTCCTCT ACTGACCAAA CTCTTGGAGA ACATCAGCCC TGCAGATGTG
    GGTGGGATGG AGGAGACTCG AATGAGGGCT TCCACACTGC TGTCTAAGGT CTTCCTGCAG
    CACCTGTCTC CACTGCTGTC ACTCTCTACC TTTGCGGCCC TCTGGCTGAC CATCCTGGAC
    TTCATGGACA AGTATATGCA TGCAGGATCC AGTGACTTAC TGTCAGAGGC CATCCCTGAG
    TCTCTGAAGA ACATGCTCCT GGTGATGGAC ACAGCAGAGA TTTTCCACAA TGCAGATGCC
    CGAGGAGGCA GCCCCTCAGC CCTCTGGGAG ATCACCTGGG AGCGCATTGA CTGTTTTCTG
    CCCCACCTAC GAGATGAGCT CTTCAAGCAG ACTGTCATCC AGGACCCCAT GCCACTGGAG
    CCACATGCCC AAAAAACTCT GGCCTCAGCC CACATGACTC CTGCTGCTGG AGACACAAGG
    ACACCTGGCC ATCCACCTCC CCCAGAGATG CCTTCTGAGC TGGGGGCCTG TGACTTCGAG
    AAGCCTGAGG GCCCTCGACC TGCCAACAGC AGCTCTCCTG GATCACCAGT GGCATCTAGC
    CCCAACAGAC TGAGCCCTAC TTCAGATGGG CCTCCTCCTC TGGCTCAGCC TCCACTGATC
    CTGCAGCCCT TGGCCTCCCC ATTGCAGGTG GGCGTACCAC CCATGACTCT ACCAATCATC
    CTCAACCCTG CTCTCATTGA GGCCACTTCA CCAGTGCCCC TCCTGGCCAC ACCCCGCCCC
    ACAGACCCAA TGCCCACCTC CGAGGTCAAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005637716.1
    CDS251..5923
    Translation

    Target ORF information:

    RefSeq Version XM_005637659.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005637659.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
    AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA
    CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT
    CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGA TTTCTCCATG TCTCAACAAA
    CTGGAGCTAA GTAGTGGGGA GCAGACCAAA GCCCTGAACC AGTTAGAGAG GGTACTACTC
    TTTAAGAACC TCAAGCTGAA AATGAGAGCA GGAGGCATGA GTGATTCATC CAAGTGGAAG
    AAACAGAAGA GATCCCCCCG GCCCCTGCGC CATATGACCA AAGTAACATC AGGTTCAGAG
    TTGCCCACCT CCAATGGAAC CACCTTATCC TCTAACCTCA CTGGGGGCAT GCCCTTCATT
    GATGTGCCCA CTCCCATCTC CTCTGCAAGT TCAGAAGCTG CCTCAACGGT GGTCAGCCCC
    TCTACAGACA GTGGCCTGGA GTTCTCCTCT CAGACCACCT CCAAGGAGGA CCTCACTGAC
    CTGGAGCAAC CTGGCTCTCC AGGGTATAGC ACAGCTACAG AGCCTGGTAG CAGTGAGCTA
    GGGGTTCCTG AGCAGCCTGA CCCGCAGCAG GAAGGAGTCC ATGTGGAAAA GGCCCAGTCA
    GCATCTGTGG AATCCATCCC CGAAGTGTTA GAGGAGTGCA CGTCTCCTGC TGACCACTCT
    GACTCTGCCT CTGTCCATGA CATGGATTAC GTCAATCCCC GGGGTGTGCG CTTTACACAG
    TCCTCCCAGA AAGAAGGTAC AGCTTTGGTT CCCTATGGTC TTCCCTGCAT CCGTGAGCTC
    TTCCGCTTCC TCATCTCCCT CACCAATCCA CATGACCGCC ACAATTCAGA AGTTATGATC
    CACATGGGAC TGCATTTGCT GACAGTGGCT CTTGAATCTG CCCCTGTAGC CCAGTGCCAA
    ACCCTTTTGG GCCTCATCAA AGATGAGATG TGCCGCCACT TACTCCAGCT ACTCAGTGTA
    GAGCGACTCA ACCTTTATGC TGCTTCCCTG CGGGTATGCT TCCTGCTTTT TGAGAGCATG
    AGGGAACATC TCAAGTTCCA ATTGGAGATG TACATCAAAA AGCTCATGGA GATCATCACT
    GTGGAGAACC CCAAAATGCC TTATGAGATG AAAGAGATGG CACTGGAGGC CATTGTGCAG
    CTCTGGCACA TCCCCAGTTT TGTCACTGAG CTCTATATCA ACTATGATTG TGACTACTAC
    TGTTCAAACC TCTTTGAAGA CCTCACCAAG CTGCTGTCCA AGAATGCCTT CCCTGTGTCT
    GGTCAGCTCT ATACAACACA CCTCCTGTCT CTTGATGCCC TGTTGACAGT GATTGACAGC
    ACTGAGGCCC ATTGCCAGGC CAAAGTCCTC AACAGTCTTA CCCAGCAAGA GAAGAAAGAA
    GCAGCCAGAT CTGGCTATGA GGCGGTGGAT GGTACCCGAG AATCCAGCAA TAGTGAGAGA
    GCAGCCAGCG ATGGGAAACC TGTAGGCATA GCCGCAGACA TCCCAGGTCT ACATCTGCCA
    GGTGGAGGGC GGCTGCCAGC AGAACATGGG AAGCCAGGAT GCAGTGATCT GGAGGAAGCT
    GCTGACTCTG GGGCTGACAA AAAGTTTACC CGGAAGCCAC CTCGATTTTC CTGTCTCCTG
    CCAGATCCAC GGGAACTGAT TGAAATTAAA AACAAAAAGA AGCTGCTAAT CACTGGCACA
    GAGCAGTTCA ACCAGAAACC AAAGAAGGGA ATCCAGTTTC TGCAGGAGAA AGGCCTCCTC
    ACCATCCCAA TGGACAACAC AGAGGTAGCC CAGTGGCTCC GAGAGAACCC TCGGCTGGAC
    AAGAAAATGA TTGGAGAGTT TGTGAGTGAC CGCAAAAACA TTGACCTGTT GGAGAGCTTT
    GTGAGCACCT TCAGCTTTCA GGGTCTGCGG CTGGATGAAG CTCTTCGTCT CTACCTGGAA
    GCCTTTCGCT TACCTGGGGA AGCACCAGTC ATCCAGAGGT TGCTGGAGGC ATTCACAGAG
    CATTGGAGGA ATTGTAATGG CTCCCCATTT GCCAATAGCG ATGCCTGCTT TGCTCTGGCC
    TATGCTGTCA TCTTGCTTAA TACTGACCAG CACAACCACA ACGTTCGCAA ACAGAATGCA
    CCCATGACTC TAGAGGAGTT TCGCAAAAAC CTAAAAGGTG TGAATGGAGG CAAGGACTTT
    GAGCAAGACA TCCTGGAGGA CATGTACCAT GCCATCAAGA ATGAGGAAAT TGTGATGCCT
    GAAGAGCAGA CAGGCTTGGT TCGGGAGAAC TATGTGTGGA ATGTGCTACT TCATCGAGGT
    GCCACCCCAG AGGGCATATT CCTTCGTGTG CCTGCTGGCA GCTATGATCT TGACCTCTTC
    ACCATGACGT GGGGCCCCAC TATTGCTGCC CTTTCTTATG TCTTTGACAA AAGCCTTGAG
    GAAACAATTA TCCAGAAAGC CATCTCAGGC TTCAGGAAGT GCGCCATGAT CTCCGCCCAC
    TATGGCCTCA GCGATGTGTT TGACAATCTC ATCATATCTC TGTGCAAATT CACAGCTCTT
    AGCAGTGAGT CTATTGAGAA TCTGCCCAGT GTGTTTGGAA GCAACCCCAA AGCCCACATT
    GCAGCCAAGA CAGTATTCCA TTTGGCCCAT CGTCATGGTG ATATCCTGCG GGAGGGCTGG
    AAGAATATCA TGGAAGCCAT GCTGCAGCTC TTCCGAGCCC AACTGCTTCC CAAAGCTATG
    GTGGAGGTAG AAGATTTTGT GGATCCCAAT GGCAAGATCT CTCTACAGAG GGAGGAGACA
    CCATCAAACC GAGGAGAGTC GACAGTGCTA AGCTTTGTGA GCTGGCTAAC ACTAAGTGGT
    ACTGAGCAGT CTAGTGTGCG GGGCCCATCC ACTGAGAACC AAGAGGCCAA AAGAGCGGCC
    TTGGACTGCA TCAAGCATTG TGACCCAGAA AAGATGATCA CAGAAAGCAA GTTCCTTCAG
    CTGGAGTCAC TGCAGGAGCT CATGAAGGCT CTGGTCTCAG TGACGCCAGA TGAAGAGACA
    TATGATGAGG AGGATGCTGC TTTCTGCCTA GAGATGCTGC TGAGGATTGT GCTGGAGAAC
    AGGGACCGTG TGGGTTGTGT GTGGCAGACT GTCCGAGACC ATCTATATCA CCTGTGTGTC
    CAGGCACAGG ATTTCTGCTT CCTTGTGGAG CGGGCAGTGG TGGGGCTGCT CCGCTTGGCC
    ATTCGACTAC TCCGAAGAGA AGAGATCAGT GGCCAGGTGC TGCTCTCCCT GCGTATTTTG
    CTACTAATGA AGCCCAGCGT GCTGTCCCGA GTCAGTCACC AGGTAGCCTA TGGGCTCCAT
    GAACTTCTTA AGACCAACGC AGCCAACATC CACTCAGGTG ACGACTGGGC CACTCTCTTC
    ACACTGCTGG AATGCATTGG CTCAGGTGTA AAGCCTCCAG CTGCCCTGCA GGCCACAGCC
    AGGGCTGAAG CACCTGATGC CGGGGCCCAA TCAGACAGCG AACTCCCATC CTACCATCAG
    AATGATGTGA GCCTGGACCG AGGATACACT TCTGACTCGG AGGTCTATGC TGACCATTGC
    AGGCCTGGCA AGATCCACCG ATCAGCCACT GATGCTGATG TAGTCAACAG CGGCTGGTTA
    GTGGTGGGGA AGGATGACAT TGATAACTCC AAGCCAGGTC CTGGGCCCAG CCGGCTAGGC
    CCTTCACCCC TGGTCAATCA GTACAGCCTA ACAGTGGGGC TGGACCTCGG GCCACACGAC
    ACTAAGTCCC TGCTTAAATG TGTAGAATCT CTGTCCTTCA TTGTGCGTGA TGCTGCCCAC
    ATTACACCTG ACAACTTTGA GCTCTGCGTG AAGACTCTCC GTATCTTTGT GGAGGCCAGT
    CTGAATGGCG GGTGCAAGTC CCAGGAGAAA CGTGGCAAGA GTCACAAATA TGACAGCAAA
    GGAAACCGCT TCAAGAAGAA ATCCAAGGAA GGTTCAGTGC TTCGGCGGCC TCGAACCTCT
    GGCCAACATG CCACTCGGGG TGGGCATAGT GACGATGATG AGGATGAAGG GGTGCCTGCC
    AGCTACCATA CGGTGTCTTT ACAGTTGTTA GATCTGATGC ACACCCTGCA CACGCGAGCA
    GCCTCTATCT ACAGCTCTTG GGCGGAGGAA CAGCGCCACC TAGAGACAGG TGGCCGGAAG
    ATAGAAGCGG ATTCACGTAC CCTCTGGGCC CACTGCTGGT GTCCCTTACT GCAGGGCATT
    GCCTGCTTGT GCTGTGATGC CCGGCGCCAG GTGCGGATGC AAGCACTGAC TTATCTGCAA
    CGAGCACTAC TGGTACATGA TCTGCAGAAG CTAGATGCCC TGGAGTGGGA GTCCTGTTTT
    AACAAGGTGC TGTTTCCTCT ACTGACCAAA CTCTTGGAGA ACATCAGCCC TGCAGATGTG
    GGTGGGATGG AGGAGACTCG AATGAGGGCT TCCACACTGC TGTCTAAGGT CTTCCTGCAG
    CACCTGTCTC CACTGCTGTC ACTCTCTACC TTTGCGGCCC TCTGGCTGAC CATCCTGGAC
    TTCATGGACA AGTATATGCA TGCAGGATCC AGTGACTTAC TGTCAGAGGC CATCCCTGAG
    TCTCTGAAGA ACATGCTCCT GGTGATGGAC ACAGCAGAGA TTTTCCACAA TGCAGATGCC
    CGAGGAGGCA GCCCCTCAGC CCTCTGGGAG ATCACCTGGG AGCGCATTGA CTGTTTTCTG
    CCCCACCTAC GAGATGAGCT CTTCAAGCAG ACTGTCATCC AGGACCCCAT GCCACTGGAG
    CCACATGCCC AAAAAACTCT GGCCTCAGCC CACATGACTC CTGCTGCTGG AGACACAAGG
    ACACCTGGCC ATCCACCTCC CCCAGAGATG CCTTCTGAGC TGGGGGCCTG TGACTTCGAG
    AAGCCTGAGG GCCCTCGACC TGCCAACAGC AGCTCTCCTG GATCACCAGT GGCATCTAGC
    CCCAACAGAC TGAGCCCTAC TTCAGATGGG CCTCCTCCTC TGGCTCAGCC TCCACTGATC
    CTGCAGCCCT TGGCCTCCCC ATTGCAGGTG GGCGTACCAC CCATGACTCT ACCAATCATC
    CTCAACCCTG CTCTCATTGA GGCCACTTCA CCAGTGCCCC TCCTGGCCAC ACCCCGCCCC
    ACAGACCCAA TGCCCACCTC CGAGGTCAAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27949
    Clone ID Related Accession (Same CDS sequence) XM_005637659.2 , XM_005637659.3
    Accession Version XM_005637659.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5673bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006610.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005637659.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
    AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA
    CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT
    CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGA TTTCTCCATG TCTCAACAAA
    CTGGAGCTAA GTAGTGGGGA GCAGACCAAA GCCCTGAACC AGTTAGAGAG GGTACTACTC
    TTTAAGAACC TCAAGCTGAA AATGAGAGCA GGAGGCATGA GTGATTCATC CAAGTGGAAG
    AAACAGAAGA GATCCCCCCG GCCCCTGCGC CATATGACCA AAGTAACATC AGGTTCAGAG
    TTGCCCACCT CCAATGGAAC CACCTTATCC TCTAACCTCA CTGGGGGCAT GCCCTTCATT
    GATGTGCCCA CTCCCATCTC CTCTGCAAGT TCAGAAGCTG CCTCAACGGT GGTCAGCCCC
    TCTACAGACA GTGGCCTGGA GTTCTCCTCT CAGACCACCT CCAAGGAGGA CCTCACTGAC
    CTGGAGCAAC CTGGCTCTCC AGGGTATAGC ACAGCTACAG AGCCTGGTAG CAGTGAGCTA
    GGGGTTCCTG AGCAGCCTGA CCCGCAGCAG GAAGGAGTCC ATGTGGAAAA GGCCCAGTCA
    GCATCTGTGG AATCCATCCC CGAAGTGTTA GAGGAGTGCA CGTCTCCTGC TGACCACTCT
    GACTCTGCCT CTGTCCATGA CATGGATTAC GTCAATCCCC GGGGTGTGCG CTTTACACAG
    TCCTCCCAGA AAGAAGGTAC AGCTTTGGTT CCCTATGGTC TTCCCTGCAT CCGTGAGCTC
    TTCCGCTTCC TCATCTCCCT CACCAATCCA CATGACCGCC ACAATTCAGA AGTTATGATC
    CACATGGGAC TGCATTTGCT GACAGTGGCT CTTGAATCTG CCCCTGTAGC CCAGTGCCAA
    ACCCTTTTGG GCCTCATCAA AGATGAGATG TGCCGCCACT TACTCCAGCT ACTCAGTGTA
    GAGCGACTCA ACCTTTATGC TGCTTCCCTG CGGGTATGCT TCCTGCTTTT TGAGAGCATG
    AGGGAACATC TCAAGTTCCA ATTGGAGATG TACATCAAAA AGCTCATGGA GATCATCACT
    GTGGAGAACC CCAAAATGCC TTATGAGATG AAAGAGATGG CACTGGAGGC CATTGTGCAG
    CTCTGGCACA TCCCCAGTTT TGTCACTGAG CTCTATATCA ACTATGATTG TGACTACTAC
    TGTTCAAACC TCTTTGAAGA CCTCACCAAG CTGCTGTCCA AGAATGCCTT CCCTGTGTCT
    GGTCAGCTCT ATACAACACA CCTCCTGTCT CTTGATGCCC TGTTGACAGT GATTGACAGC
    ACTGAGGCCC ATTGCCAGGC CAAAGTCCTC AACAGTCTTA CCCAGCAAGA GAAGAAAGAA
    GCAGCCAGAT CTGGCTATGA GGCGGTGGAT GGTACCCGAG AATCCAGCAA TAGTGAGAGA
    GCAGCCAGCG ATGGGAAACC TGTAGGCATA GCCGCAGACA TCCCAGGTCT ACATCTGCCA
    GGTGGAGGGC GGCTGCCAGC AGAACATGGG AAGCCAGGAT GCAGTGATCT GGAGGAAGCT
    GCTGACTCTG GGGCTGACAA AAAGTTTACC CGGAAGCCAC CTCGATTTTC CTGTCTCCTG
    CCAGATCCAC GGGAACTGAT TGAAATTAAA AACAAAAAGA AGCTGCTAAT CACTGGCACA
    GAGCAGTTCA ACCAGAAACC AAAGAAGGGA ATCCAGTTTC TGCAGGAGAA AGGCCTCCTC
    ACCATCCCAA TGGACAACAC AGAGGTAGCC CAGTGGCTCC GAGAGAACCC TCGGCTGGAC
    AAGAAAATGA TTGGAGAGTT TGTGAGTGAC CGCAAAAACA TTGACCTGTT GGAGAGCTTT
    GTGAGCACCT TCAGCTTTCA GGGTCTGCGG CTGGATGAAG CTCTTCGTCT CTACCTGGAA
    GCCTTTCGCT TACCTGGGGA AGCACCAGTC ATCCAGAGGT TGCTGGAGGC ATTCACAGAG
    CATTGGAGGA ATTGTAATGG CTCCCCATTT GCCAATAGCG ATGCCTGCTT TGCTCTGGCC
    TATGCTGTCA TCTTGCTTAA TACTGACCAG CACAACCACA ACGTTCGCAA ACAGAATGCA
    CCCATGACTC TAGAGGAGTT TCGCAAAAAC CTAAAAGGTG TGAATGGAGG CAAGGACTTT
    GAGCAAGACA TCCTGGAGGA CATGTACCAT GCCATCAAGA ATGAGGAAAT TGTGATGCCT
    GAAGAGCAGA CAGGCTTGGT TCGGGAGAAC TATGTGTGGA ATGTGCTACT TCATCGAGGT
    GCCACCCCAG AGGGCATATT CCTTCGTGTG CCTGCTGGCA GCTATGATCT TGACCTCTTC
    ACCATGACGT GGGGCCCCAC TATTGCTGCC CTTTCTTATG TCTTTGACAA AAGCCTTGAG
    GAAACAATTA TCCAGAAAGC CATCTCAGGC TTCAGGAAGT GCGCCATGAT CTCCGCCCAC
    TATGGCCTCA GCGATGTGTT TGACAATCTC ATCATATCTC TGTGCAAATT CACAGCTCTT
    AGCAGTGAGT CTATTGAGAA TCTGCCCAGT GTGTTTGGAA GCAACCCCAA AGCCCACATT
    GCAGCCAAGA CAGTATTCCA TTTGGCCCAT CGTCATGGTG ATATCCTGCG GGAGGGCTGG
    AAGAATATCA TGGAAGCCAT GCTGCAGCTC TTCCGAGCCC AACTGCTTCC CAAAGCTATG
    GTGGAGGTAG AAGATTTTGT GGATCCCAAT GGCAAGATCT CTCTACAGAG GGAGGAGACA
    CCATCAAACC GAGGAGAGTC GACAGTGCTA AGCTTTGTGA GCTGGCTAAC ACTAAGTGGT
    ACTGAGCAGT CTAGTGTGCG GGGCCCATCC ACTGAGAACC AAGAGGCCAA AAGAGCGGCC
    TTGGACTGCA TCAAGCATTG TGACCCAGAA AAGATGATCA CAGAAAGCAA GTTCCTTCAG
    CTGGAGTCAC TGCAGGAGCT CATGAAGGCT CTGGTCTCAG TGACGCCAGA TGAAGAGACA
    TATGATGAGG AGGATGCTGC TTTCTGCCTA GAGATGCTGC TGAGGATTGT GCTGGAGAAC
    AGGGACCGTG TGGGTTGTGT GTGGCAGACT GTCCGAGACC ATCTATATCA CCTGTGTGTC
    CAGGCACAGG ATTTCTGCTT CCTTGTGGAG CGGGCAGTGG TGGGGCTGCT CCGCTTGGCC
    ATTCGACTAC TCCGAAGAGA AGAGATCAGT GGCCAGGTGC TGCTCTCCCT GCGTATTTTG
    CTACTAATGA AGCCCAGCGT GCTGTCCCGA GTCAGTCACC AGGTAGCCTA TGGGCTCCAT
    GAACTTCTTA AGACCAACGC AGCCAACATC CACTCAGGTG ACGACTGGGC CACTCTCTTC
    ACACTGCTGG AATGCATTGG CTCAGGTGTA AAGCCTCCAG CTGCCCTGCA GGCCACAGCC
    AGGGCTGAAG CACCTGATGC CGGGGCCCAA TCAGACAGCG AACTCCCATC CTACCATCAG
    AATGATGTGA GCCTGGACCG AGGATACACT TCTGACTCGG AGGTCTATGC TGACCATTGC
    AGGCCTGGCA AGATCCACCG ATCAGCCACT GATGCTGATG TAGTCAACAG CGGCTGGTTA
    GTGGTGGGGA AGGATGACAT TGATAACTCC AAGCCAGGTC CTGGGCCCAG CCGGCTAGGC
    CCTTCACCCC TGGTCAATCA GTACAGCCTA ACAGTGGGGC TGGACCTCGG GCCACACGAC
    ACTAAGTCCC TGCTTAAATG TGTAGAATCT CTGTCCTTCA TTGTGCGTGA TGCTGCCCAC
    ATTACACCTG ACAACTTTGA GCTCTGCGTG AAGACTCTCC GTATCTTTGT GGAGGCCAGT
    CTGAATGGCG GGTGCAAGTC CCAGGAGAAA CGTGGCAAGA GTCACAAATA TGACAGCAAA
    GGAAACCGCT TCAAGAAGAA ATCCAAGGAA GGTTCAGTGC TTCGGCGGCC TCGAACCTCT
    GGCCAACATG CCACTCGGGG TGGGCATAGT GACGATGATG AGGATGAAGG GGTGCCTGCC
    AGCTACCATA CGGTGTCTTT ACAGTTGTTA GATCTGATGC ACACCCTGCA CACGCGAGCA
    GCCTCTATCT ACAGCTCTTG GGCGGAGGAA CAGCGCCACC TAGAGACAGG TGGCCGGAAG
    ATAGAAGCGG ATTCACGTAC CCTCTGGGCC CACTGCTGGT GTCCCTTACT GCAGGGCATT
    GCCTGCTTGT GCTGTGATGC CCGGCGCCAG GTGCGGATGC AAGCACTGAC TTATCTGCAA
    CGAGCACTAC TGGTACATGA TCTGCAGAAG CTAGATGCCC TGGAGTGGGA GTCCTGTTTT
    AACAAGGTGC TGTTTCCTCT ACTGACCAAA CTCTTGGAGA ACATCAGCCC TGCAGATGTG
    GGTGGGATGG AGGAGACTCG AATGAGGGCT TCCACACTGC TGTCTAAGGT CTTCCTGCAG
    CACCTGTCTC CACTGCTGTC ACTCTCTACC TTTGCGGCCC TCTGGCTGAC CATCCTGGAC
    TTCATGGACA AGTATATGCA TGCAGGATCC AGTGACTTAC TGTCAGAGGC CATCCCTGAG
    TCTCTGAAGA ACATGCTCCT GGTGATGGAC ACAGCAGAGA TTTTCCACAA TGCAGATGCC
    CGAGGAGGCA GCCCCTCAGC CCTCTGGGAG ATCACCTGGG AGCGCATTGA CTGTTTTCTG
    CCCCACCTAC GAGATGAGCT CTTCAAGCAG ACTGTCATCC AGGACCCCAT GCCACTGGAG
    CCACATGCCC AAAAAACTCT GGCCTCAGCC CACATGACTC CTGCTGCTGG AGACACAAGG
    ACACCTGGCC ATCCACCTCC CCCAGAGATG CCTTCTGAGC TGGGGGCCTG TGACTTCGAG
    AAGCCTGAGG GCCCTCGACC TGCCAACAGC AGCTCTCCTG GATCACCAGT GGCATCTAGC
    CCCAACAGAC TGAGCCCTAC TTCAGATGGG CCTCCTCCTC TGGCTCAGCC TCCACTGATC
    CTGCAGCCCT TGGCCTCCCC ATTGCAGGTG GGCGTACCAC CCATGACTCT ACCAATCATC
    CTCAACCCTG CTCTCATTGA GGCCACTTCA CCAGTGCCCC TCCTGGCCAC ACCCCGCCCC
    ACAGACCCAA TGCCCACCTC CGAGGTCAAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005637716.1
    CDS269..5941
    Translation

    Target ORF information:

    RefSeq Version XM_005637659.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005637659.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
    AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA
    CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT
    CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGA TTTCTCCATG TCTCAACAAA
    CTGGAGCTAA GTAGTGGGGA GCAGACCAAA GCCCTGAACC AGTTAGAGAG GGTACTACTC
    TTTAAGAACC TCAAGCTGAA AATGAGAGCA GGAGGCATGA GTGATTCATC CAAGTGGAAG
    AAACAGAAGA GATCCCCCCG GCCCCTGCGC CATATGACCA AAGTAACATC AGGTTCAGAG
    TTGCCCACCT CCAATGGAAC CACCTTATCC TCTAACCTCA CTGGGGGCAT GCCCTTCATT
    GATGTGCCCA CTCCCATCTC CTCTGCAAGT TCAGAAGCTG CCTCAACGGT GGTCAGCCCC
    TCTACAGACA GTGGCCTGGA GTTCTCCTCT CAGACCACCT CCAAGGAGGA CCTCACTGAC
    CTGGAGCAAC CTGGCTCTCC AGGGTATAGC ACAGCTACAG AGCCTGGTAG CAGTGAGCTA
    GGGGTTCCTG AGCAGCCTGA CCCGCAGCAG GAAGGAGTCC ATGTGGAAAA GGCCCAGTCA
    GCATCTGTGG AATCCATCCC CGAAGTGTTA GAGGAGTGCA CGTCTCCTGC TGACCACTCT
    GACTCTGCCT CTGTCCATGA CATGGATTAC GTCAATCCCC GGGGTGTGCG CTTTACACAG
    TCCTCCCAGA AAGAAGGTAC AGCTTTGGTT CCCTATGGTC TTCCCTGCAT CCGTGAGCTC
    TTCCGCTTCC TCATCTCCCT CACCAATCCA CATGACCGCC ACAATTCAGA AGTTATGATC
    CACATGGGAC TGCATTTGCT GACAGTGGCT CTTGAATCTG CCCCTGTAGC CCAGTGCCAA
    ACCCTTTTGG GCCTCATCAA AGATGAGATG TGCCGCCACT TACTCCAGCT ACTCAGTGTA
    GAGCGACTCA ACCTTTATGC TGCTTCCCTG CGGGTATGCT TCCTGCTTTT TGAGAGCATG
    AGGGAACATC TCAAGTTCCA ATTGGAGATG TACATCAAAA AGCTCATGGA GATCATCACT
    GTGGAGAACC CCAAAATGCC TTATGAGATG AAAGAGATGG CACTGGAGGC CATTGTGCAG
    CTCTGGCACA TCCCCAGTTT TGTCACTGAG CTCTATATCA ACTATGATTG TGACTACTAC
    TGTTCAAACC TCTTTGAAGA CCTCACCAAG CTGCTGTCCA AGAATGCCTT CCCTGTGTCT
    GGTCAGCTCT ATACAACACA CCTCCTGTCT CTTGATGCCC TGTTGACAGT GATTGACAGC
    ACTGAGGCCC ATTGCCAGGC CAAAGTCCTC AACAGTCTTA CCCAGCAAGA GAAGAAAGAA
    GCAGCCAGAT CTGGCTATGA GGCGGTGGAT GGTACCCGAG AATCCAGCAA TAGTGAGAGA
    GCAGCCAGCG ATGGGAAACC TGTAGGCATA GCCGCAGACA TCCCAGGTCT ACATCTGCCA
    GGTGGAGGGC GGCTGCCAGC AGAACATGGG AAGCCAGGAT GCAGTGATCT GGAGGAAGCT
    GCTGACTCTG GGGCTGACAA AAAGTTTACC CGGAAGCCAC CTCGATTTTC CTGTCTCCTG
    CCAGATCCAC GGGAACTGAT TGAAATTAAA AACAAAAAGA AGCTGCTAAT CACTGGCACA
    GAGCAGTTCA ACCAGAAACC AAAGAAGGGA ATCCAGTTTC TGCAGGAGAA AGGCCTCCTC
    ACCATCCCAA TGGACAACAC AGAGGTAGCC CAGTGGCTCC GAGAGAACCC TCGGCTGGAC
    AAGAAAATGA TTGGAGAGTT TGTGAGTGAC CGCAAAAACA TTGACCTGTT GGAGAGCTTT
    GTGAGCACCT TCAGCTTTCA GGGTCTGCGG CTGGATGAAG CTCTTCGTCT CTACCTGGAA
    GCCTTTCGCT TACCTGGGGA AGCACCAGTC ATCCAGAGGT TGCTGGAGGC ATTCACAGAG
    CATTGGAGGA ATTGTAATGG CTCCCCATTT GCCAATAGCG ATGCCTGCTT TGCTCTGGCC
    TATGCTGTCA TCTTGCTTAA TACTGACCAG CACAACCACA ACGTTCGCAA ACAGAATGCA
    CCCATGACTC TAGAGGAGTT TCGCAAAAAC CTAAAAGGTG TGAATGGAGG CAAGGACTTT
    GAGCAAGACA TCCTGGAGGA CATGTACCAT GCCATCAAGA ATGAGGAAAT TGTGATGCCT
    GAAGAGCAGA CAGGCTTGGT TCGGGAGAAC TATGTGTGGA ATGTGCTACT TCATCGAGGT
    GCCACCCCAG AGGGCATATT CCTTCGTGTG CCTGCTGGCA GCTATGATCT TGACCTCTTC
    ACCATGACGT GGGGCCCCAC TATTGCTGCC CTTTCTTATG TCTTTGACAA AAGCCTTGAG
    GAAACAATTA TCCAGAAAGC CATCTCAGGC TTCAGGAAGT GCGCCATGAT CTCCGCCCAC
    TATGGCCTCA GCGATGTGTT TGACAATCTC ATCATATCTC TGTGCAAATT CACAGCTCTT
    AGCAGTGAGT CTATTGAGAA TCTGCCCAGT GTGTTTGGAA GCAACCCCAA AGCCCACATT
    GCAGCCAAGA CAGTATTCCA TTTGGCCCAT CGTCATGGTG ATATCCTGCG GGAGGGCTGG
    AAGAATATCA TGGAAGCCAT GCTGCAGCTC TTCCGAGCCC AACTGCTTCC CAAAGCTATG
    GTGGAGGTAG AAGATTTTGT GGATCCCAAT GGCAAGATCT CTCTACAGAG GGAGGAGACA
    CCATCAAACC GAGGAGAGTC GACAGTGCTA AGCTTTGTGA GCTGGCTAAC ACTAAGTGGT
    ACTGAGCAGT CTAGTGTGCG GGGCCCATCC ACTGAGAACC AAGAGGCCAA AAGAGCGGCC
    TTGGACTGCA TCAAGCATTG TGACCCAGAA AAGATGATCA CAGAAAGCAA GTTCCTTCAG
    CTGGAGTCAC TGCAGGAGCT CATGAAGGCT CTGGTCTCAG TGACGCCAGA TGAAGAGACA
    TATGATGAGG AGGATGCTGC TTTCTGCCTA GAGATGCTGC TGAGGATTGT GCTGGAGAAC
    AGGGACCGTG TGGGTTGTGT GTGGCAGACT GTCCGAGACC ATCTATATCA CCTGTGTGTC
    CAGGCACAGG ATTTCTGCTT CCTTGTGGAG CGGGCAGTGG TGGGGCTGCT CCGCTTGGCC
    ATTCGACTAC TCCGAAGAGA AGAGATCAGT GGCCAGGTGC TGCTCTCCCT GCGTATTTTG
    CTACTAATGA AGCCCAGCGT GCTGTCCCGA GTCAGTCACC AGGTAGCCTA TGGGCTCCAT
    GAACTTCTTA AGACCAACGC AGCCAACATC CACTCAGGTG ACGACTGGGC CACTCTCTTC
    ACACTGCTGG AATGCATTGG CTCAGGTGTA AAGCCTCCAG CTGCCCTGCA GGCCACAGCC
    AGGGCTGAAG CACCTGATGC CGGGGCCCAA TCAGACAGCG AACTCCCATC CTACCATCAG
    AATGATGTGA GCCTGGACCG AGGATACACT TCTGACTCGG AGGTCTATGC TGACCATTGC
    AGGCCTGGCA AGATCCACCG ATCAGCCACT GATGCTGATG TAGTCAACAG CGGCTGGTTA
    GTGGTGGGGA AGGATGACAT TGATAACTCC AAGCCAGGTC CTGGGCCCAG CCGGCTAGGC
    CCTTCACCCC TGGTCAATCA GTACAGCCTA ACAGTGGGGC TGGACCTCGG GCCACACGAC
    ACTAAGTCCC TGCTTAAATG TGTAGAATCT CTGTCCTTCA TTGTGCGTGA TGCTGCCCAC
    ATTACACCTG ACAACTTTGA GCTCTGCGTG AAGACTCTCC GTATCTTTGT GGAGGCCAGT
    CTGAATGGCG GGTGCAAGTC CCAGGAGAAA CGTGGCAAGA GTCACAAATA TGACAGCAAA
    GGAAACCGCT TCAAGAAGAA ATCCAAGGAA GGTTCAGTGC TTCGGCGGCC TCGAACCTCT
    GGCCAACATG CCACTCGGGG TGGGCATAGT GACGATGATG AGGATGAAGG GGTGCCTGCC
    AGCTACCATA CGGTGTCTTT ACAGTTGTTA GATCTGATGC ACACCCTGCA CACGCGAGCA
    GCCTCTATCT ACAGCTCTTG GGCGGAGGAA CAGCGCCACC TAGAGACAGG TGGCCGGAAG
    ATAGAAGCGG ATTCACGTAC CCTCTGGGCC CACTGCTGGT GTCCCTTACT GCAGGGCATT
    GCCTGCTTGT GCTGTGATGC CCGGCGCCAG GTGCGGATGC AAGCACTGAC TTATCTGCAA
    CGAGCACTAC TGGTACATGA TCTGCAGAAG CTAGATGCCC TGGAGTGGGA GTCCTGTTTT
    AACAAGGTGC TGTTTCCTCT ACTGACCAAA CTCTTGGAGA ACATCAGCCC TGCAGATGTG
    GGTGGGATGG AGGAGACTCG AATGAGGGCT TCCACACTGC TGTCTAAGGT CTTCCTGCAG
    CACCTGTCTC CACTGCTGTC ACTCTCTACC TTTGCGGCCC TCTGGCTGAC CATCCTGGAC
    TTCATGGACA AGTATATGCA TGCAGGATCC AGTGACTTAC TGTCAGAGGC CATCCCTGAG
    TCTCTGAAGA ACATGCTCCT GGTGATGGAC ACAGCAGAGA TTTTCCACAA TGCAGATGCC
    CGAGGAGGCA GCCCCTCAGC CCTCTGGGAG ATCACCTGGG AGCGCATTGA CTGTTTTCTG
    CCCCACCTAC GAGATGAGCT CTTCAAGCAG ACTGTCATCC AGGACCCCAT GCCACTGGAG
    CCACATGCCC AAAAAACTCT GGCCTCAGCC CACATGACTC CTGCTGCTGG AGACACAAGG
    ACACCTGGCC ATCCACCTCC CCCAGAGATG CCTTCTGAGC TGGGGGCCTG TGACTTCGAG
    AAGCCTGAGG GCCCTCGACC TGCCAACAGC AGCTCTCCTG GATCACCAGT GGCATCTAGC
    CCCAACAGAC TGAGCCCTAC TTCAGATGGG CCTCCTCCTC TGGCTCAGCC TCCACTGATC
    CTGCAGCCCT TGGCCTCCCC ATTGCAGGTG GGCGTACCAC CCATGACTCT ACCAATCATC
    CTCAACCCTG CTCTCATTGA GGCCACTTCA CCAGTGCCCC TCCTGGCCAC ACCCCGCCCC
    ACAGACCCAA TGCCCACCTC CGAGGTCAAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27950
    Clone ID Related Accession (Same CDS sequence) XM_005637660.3 , XM_005637660.2
    Accession Version XM_005637660.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5661bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006610.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005637660.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
    AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA
    CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT
    CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGA TTTCTCCATG TCTCAACAAA
    CTGGAGCTAA GTAGTGGGGA GCAGACCAAA GCCCTGAACC AGTTAGAGAG GCTGAAAATG
    AGAGCAGGAG GCATGAGTGA TTCATCCAAG TGGAAGAAAC AGAAGAGATC CCCCCGGCCC
    CTGCGCCATA TGACCAAAGT AACATCAGGT TCAGAGTTGC CCACCTCCAA TGGAACCACC
    TTATCCTCTA ACCTCACTGG GGGCATGCCC TTCATTGATG TGCCCACTCC CATCTCCTCT
    GCAAGTTCAG AAGCTGCCTC AACGGTGGTC AGCCCCTCTA CAGACAGTGG CCTGGAGTTC
    TCCTCTCAGA CCACCTCCAA GGAGGACCTC ACTGACCTGG AGCAACCTGG CTCTCCAGGG
    TATAGCACAG CTACAGAGCC TGGTAGCAGT GAGCTAGGGG TTCCTGAGCA GCCTGACCCG
    CAGCAGGAAG GAGTCCATGT GGAAAAGGCC CAGTCAGCAT CTGTGGAATC CATCCCCGAA
    GTGTTAGAGG AGTGCACGTC TCCTGCTGAC CACTCTGACT CTGCCTCTGT CCATGACATG
    GATTACGTCA ATCCCCGGGG TGTGCGCTTT ACACAGTCCT CCCAGAAAGA AGGTACAGCT
    TTGGTTCCCT ATGGTCTTCC CTGCATCCGT GAGCTCTTCC GCTTCCTCAT CTCCCTCACC
    AATCCACATG ACCGCCACAA TTCAGAAGTT ATGATCCACA TGGGACTGCA TTTGCTGACA
    GTGGCTCTTG AATCTGCCCC TGTAGCCCAG TGCCAAACCC TTTTGGGCCT CATCAAAGAT
    GAGATGTGCC GCCACTTACT CCAGCTACTC AGTGTAGAGC GACTCAACCT TTATGCTGCT
    TCCCTGCGGG TATGCTTCCT GCTTTTTGAG AGCATGAGGG AACATCTCAA GTTCCAATTG
    GAGATGTACA TCAAAAAGCT CATGGAGATC ATCACTGTGG AGAACCCCAA AATGCCTTAT
    GAGATGAAAG AGATGGCACT GGAGGCCATT GTGCAGCTCT GGCACATCCC CAGTTTTGTC
    ACTGAGCTCT ATATCAACTA TGATTGTGAC TACTACTGTT CAAACCTCTT TGAAGACCTC
    ACCAAGCTGC TGTCCAAGAA TGCCTTCCCT GTGTCTGGTC AGCTCTATAC AACACACCTC
    CTGTCTCTTG ATGCCCTGTT GACAGTGATT GACAGCACTG AGGCCCATTG CCAGGCCAAA
    GTCCTCAACA GTCTTACCCA GCAAGAGAAG AAAGAAGCAG CCAGATCTGG CTATGAGGCG
    GTGGATGGTA CCCGAGAATC CAGCAATAGT GAGAGAGCAG CCAGCGATGG GAAACCTGTA
    GGCATAGCCG CAGACATCCC AGGTCTACAT CTGCCAGGTG GAGGGCGGCT GCCAGCAGAA
    CATGGGAAGC CAGGATGCAG TGATCTGGAG GAAGCTGCTG ACTCTGGGGC TGACAAAAAG
    TTTACCCGGA AGCCACCTCG ATTTTCCTGT CTCCTGCCAG ATCCACGGGA ACTGATTGAA
    ATTAAAAACA AAAAGAAGCT GCTAATCACT GGCACAGAGC AGTTCAACCA GAAACCAAAG
    AAGGGAATCC AGTTTCTGCA GGAGAAAGGC CTCCTCACCA TCCCAATGGA CAACACAGAG
    GTAGCCCAGT GGCTCCGAGA GAACCCTCGG CTGGACAAGA AAATGATTGG AGAGTTTGTG
    AGTGACCGCA AAAACATTGA CCTGTTGGAG AGCTTTGTGA GCACCTTCAG CTTTCAGGGT
    CTGCGGCTGG ATGAAGCTCT TCGTCTCTAC CTGGAAGCCT TTCGCTTACC TGGGGAAGCA
    CCAGTCATCC AGAGGTTGCT GGAGGCATTC ACAGAGCATT GGAGGAATTG TAATGGCTCC
    CCATTTGCCA ATAGCGATGC CTGCTTTGCT CTGGCCTATG CTGTCATCTT GCTTAATACT
    GACCAGCACA ACCACAACGT TCGCAAACAG AATGCACCCA TGACTCTAGA GGAGTTTCGC
    AAAAACCTAA AAGGTGTGAA TGGAGGCAAG GACTTTGAGC AAGACATCCT GGAGGACATG
    TACCATGCCA TCAAGAATGA GGAAATTGTG ATGCCTGAAG AGCAGACAGG CTTGGTTCGG
    GAGAACTATG TGTGGAATGT GCTACTTCAT CGAGGTGCCA CCCCAGAGGG CATATTCCTT
    CGTGTGCCTG CTGGCAGCTA TGATCTTGAC CTCTTCACCA TGACGTGGGG CCCCACTATT
    GCTGCCCTTT CTTATGTCTT TGACAAAAGC CTTGAGGAAA CAATTATCCA GAAAGCCATC
    TCAGGCTTCA GGAAGTGCGC CATGATCTCC GCCCACTATG GCCTCAGCGA TGTGTTTGAC
    AATCTCATCA TATCTCTGTG CAAATTCACA GCTCTTAGCA GTGAGTCTAT TGAGAATCTG
    CCCAGTGTGT TTGGAAGCAA CCCCAAAGCC CACATTGCAG CCAAGACAGT ATTCCATTTG
    GCCCATCGTC ATGGTGATAT CCTGCGGGAG GGCTGGAAGA ATATCATGGA AGCCATGCTG
    CAGCTCTTCC GAGCCCAACT GCTTCCCAAA GCTATGGTGG AGGTAGAAGA TTTTGTGGAT
    CCCAATGGCA AGATCTCTCT ACAGAGGGAG GAGACACCAT CAAACCGAGG AGAGTCGACA
    GTGCTAAGCT TTGTGAGCTG GCTAACACTA AGTGGTACTG AGCAGTCTAG TGTGCGGGGC
    CCATCCACTG AGAACCAAGA GGCCAAAAGA GCGGCCTTGG ACTGCATCAA GCATTGTGAC
    CCAGAAAAGA TGATCACAGA AAGCAAGTTC CTTCAGCTGG AGTCACTGCA GGAGCTCATG
    AAGGCTCTGG TCTCAGTGAC GCCAGATGAA GAGACATATG ATGAGGAGGA TGCTGCTTTC
    TGCCTAGAGA TGCTGCTGAG GATTGTGCTG GAGAACAGGG ACCGTGTGGG TTGTGTGTGG
    CAGACTGTCC GAGACCATCT ATATCACCTG TGTGTCCAGG CACAGGATTT CTGCTTCCTT
    GTGGAGCGGG CAGTGGTGGG GCTGCTCCGC TTGGCCATTC GACTACTCCG AAGAGAAGAG
    ATCAGTGGCC AGGTGCTGCT CTCCCTGCGT ATTTTGCTAC TAATGAAGCC CAGCGTGCTG
    TCCCGAGTCA GTCACCAGGT AGCCTATGGG CTCCATGAAC TTCTTAAGAC CAACGCAGCC
    AACATCCACT CAGGTGACGA CTGGGCCACT CTCTTCACAC TGCTGGAATG CATTGGCTCA
    GGTGTAAAGC CTCCAGCTGC CCTGCAGGCC ACAGCCAGGG CTGAAGCACC TGATGCCGGG
    GCCCAATCAG ACAGCGAACT CCCATCCTAC CATCAGAATG ATGTGAGCCT GGACCGAGGA
    TACACTTCTG ACTCGGAGGT CTATGCTGAC CATTGCAGGC CTGGCAAGAT CCACCGATCA
    GCCACTGATG CTGATGTAGT CAACAGCGGC TGGTTAGTGG TGGGGAAGGA TGACATTGAT
    AACTCCAAGC CAGGTCCTGG GCCCAGCCGG CTAGGCCCTT CACCCCTGGT CAATCAGTAC
    AGCCTAACAG TGGGGCTGGA CCTCGGGCCA CACGACACTA AGTCCCTGCT TAAATGTGTA
    GAATCTCTGT CCTTCATTGT GCGTGATGCT GCCCACATTA CACCTGACAA CTTTGAGCTC
    TGCGTGAAGA CTCTCCGTAT CTTTGTGGAG GCCAGTCTGA ATGGCGGGTG CAAGTCCCAG
    GAGAAACGTG GCAAGAGTCA CAAATATGAC AGCAAAGGAA ACCGCTTCAA GAAGAAATCC
    AAGGAAGGTT CAGTGCTTCG GCGGCCTCGA ACCTCTGGCC AACATGCCAC TCGGGGTGGG
    CATAGTGACG ATGATGAGGA TGAAGGGGTG CCTGCCAGCT ACCATACGGT GTCTTTACAG
    GTCAGTCAGG ACTTGTTAGA TCTGATGCAC ACCCTGCACA CGCGAGCAGC CTCTATCTAC
    AGCTCTTGGG CGGAGGAACA GCGCCACCTA GAGACAGGTG GCCGGAAGAT AGAAGCGGAT
    TCACGTACCC TCTGGGCCCA CTGCTGGTGT CCCTTACTGC AGGGCATTGC CTGCTTGTGC
    TGTGATGCCC GGCGCCAGGT GCGGATGCAA GCACTGACTT ATCTGCAACG AGCACTACTG
    GTACATGATC TGCAGAAGCT AGATGCCCTG GAGTGGGAGT CCTGTTTTAA CAAGGTGCTG
    TTTCCTCTAC TGACCAAACT CTTGGAGAAC ATCAGCCCTG CAGATGTGGG TGGGATGGAG
    GAGACTCGAA TGAGGGCTTC CACACTGCTG TCTAAGGTCT TCCTGCAGCA CCTGTCTCCA
    CTGCTGTCAC TCTCTACCTT TGCGGCCCTC TGGCTGACCA TCCTGGACTT CATGGACAAG
    TATATGCATG CAGGATCCAG TGACTTACTG TCAGAGGCCA TCCCTGAGTC TCTGAAGAAC
    ATGCTCCTGG TGATGGACAC AGCAGAGATT TTCCACAATG CAGATGCCCG AGGAGGCAGC
    CCCTCAGCCC TCTGGGAGAT CACCTGGGAG CGCATTGACT GTTTTCTGCC CCACCTACGA
    GATGAGCTCT TCAAGCAGAC TGTCATCCAG GACCCCATGC CACTGGAGCC ACATGCCCAA
    AAAACTCTGG CCTCAGCCCA CATGACTCCT GCTGCTGGAG ACACAAGGAC ACCTGGCCAT
    CCACCTCCCC CAGAGATGCC TTCTGAGCTG GGGGCCTGTG ACTTCGAGAA GCCTGAGGGC
    CCTCGACCTG CCAACAGCAG CTCTCCTGGA TCACCAGTGG CATCTAGCCC CAACAGACTG
    AGCCCTACTT CAGATGGGCC TCCTCCTCTG GCTCAGCCTC CACTGATCCT GCAGCCCTTG
    GCCTCCCCAT TGCAGGTGGG CGTACCACCC ATGACTCTAC CAATCATCCT CAACCCTGCT
    CTCATTGAGG CCACTTCACC AGTGCCCCTC CTGGCCACAC CCCGCCCCAC AGACCCAATG
    CCCACCTCCG AGGTCAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005637717.1
    CDS269..5929
    Translation

    Target ORF information:

    RefSeq Version XM_005637660.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005637660.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
    AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA
    CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT
    CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGA TTTCTCCATG TCTCAACAAA
    CTGGAGCTAA GTAGTGGGGA GCAGACCAAA GCCCTGAACC AGTTAGAGAG GCTGAAAATG
    AGAGCAGGAG GCATGAGTGA TTCATCCAAG TGGAAGAAAC AGAAGAGATC CCCCCGGCCC
    CTGCGCCATA TGACCAAAGT AACATCAGGT TCAGAGTTGC CCACCTCCAA TGGAACCACC
    TTATCCTCTA ACCTCACTGG GGGCATGCCC TTCATTGATG TGCCCACTCC CATCTCCTCT
    GCAAGTTCAG AAGCTGCCTC AACGGTGGTC AGCCCCTCTA CAGACAGTGG CCTGGAGTTC
    TCCTCTCAGA CCACCTCCAA GGAGGACCTC ACTGACCTGG AGCAACCTGG CTCTCCAGGG
    TATAGCACAG CTACAGAGCC TGGTAGCAGT GAGCTAGGGG TTCCTGAGCA GCCTGACCCG
    CAGCAGGAAG GAGTCCATGT GGAAAAGGCC CAGTCAGCAT CTGTGGAATC CATCCCCGAA
    GTGTTAGAGG AGTGCACGTC TCCTGCTGAC CACTCTGACT CTGCCTCTGT CCATGACATG
    GATTACGTCA ATCCCCGGGG TGTGCGCTTT ACACAGTCCT CCCAGAAAGA AGGTACAGCT
    TTGGTTCCCT ATGGTCTTCC CTGCATCCGT GAGCTCTTCC GCTTCCTCAT CTCCCTCACC
    AATCCACATG ACCGCCACAA TTCAGAAGTT ATGATCCACA TGGGACTGCA TTTGCTGACA
    GTGGCTCTTG AATCTGCCCC TGTAGCCCAG TGCCAAACCC TTTTGGGCCT CATCAAAGAT
    GAGATGTGCC GCCACTTACT CCAGCTACTC AGTGTAGAGC GACTCAACCT TTATGCTGCT
    TCCCTGCGGG TATGCTTCCT GCTTTTTGAG AGCATGAGGG AACATCTCAA GTTCCAATTG
    GAGATGTACA TCAAAAAGCT CATGGAGATC ATCACTGTGG AGAACCCCAA AATGCCTTAT
    GAGATGAAAG AGATGGCACT GGAGGCCATT GTGCAGCTCT GGCACATCCC CAGTTTTGTC
    ACTGAGCTCT ATATCAACTA TGATTGTGAC TACTACTGTT CAAACCTCTT TGAAGACCTC
    ACCAAGCTGC TGTCCAAGAA TGCCTTCCCT GTGTCTGGTC AGCTCTATAC AACACACCTC
    CTGTCTCTTG ATGCCCTGTT GACAGTGATT GACAGCACTG AGGCCCATTG CCAGGCCAAA
    GTCCTCAACA GTCTTACCCA GCAAGAGAAG AAAGAAGCAG CCAGATCTGG CTATGAGGCG
    GTGGATGGTA CCCGAGAATC CAGCAATAGT GAGAGAGCAG CCAGCGATGG GAAACCTGTA
    GGCATAGCCG CAGACATCCC AGGTCTACAT CTGCCAGGTG GAGGGCGGCT GCCAGCAGAA
    CATGGGAAGC CAGGATGCAG TGATCTGGAG GAAGCTGCTG ACTCTGGGGC TGACAAAAAG
    TTTACCCGGA AGCCACCTCG ATTTTCCTGT CTCCTGCCAG ATCCACGGGA ACTGATTGAA
    ATTAAAAACA AAAAGAAGCT GCTAATCACT GGCACAGAGC AGTTCAACCA GAAACCAAAG
    AAGGGAATCC AGTTTCTGCA GGAGAAAGGC CTCCTCACCA TCCCAATGGA CAACACAGAG
    GTAGCCCAGT GGCTCCGAGA GAACCCTCGG CTGGACAAGA AAATGATTGG AGAGTTTGTG
    AGTGACCGCA AAAACATTGA CCTGTTGGAG AGCTTTGTGA GCACCTTCAG CTTTCAGGGT
    CTGCGGCTGG ATGAAGCTCT TCGTCTCTAC CTGGAAGCCT TTCGCTTACC TGGGGAAGCA
    CCAGTCATCC AGAGGTTGCT GGAGGCATTC ACAGAGCATT GGAGGAATTG TAATGGCTCC
    CCATTTGCCA ATAGCGATGC CTGCTTTGCT CTGGCCTATG CTGTCATCTT GCTTAATACT
    GACCAGCACA ACCACAACGT TCGCAAACAG AATGCACCCA TGACTCTAGA GGAGTTTCGC
    AAAAACCTAA AAGGTGTGAA TGGAGGCAAG GACTTTGAGC AAGACATCCT GGAGGACATG
    TACCATGCCA TCAAGAATGA GGAAATTGTG ATGCCTGAAG AGCAGACAGG CTTGGTTCGG
    GAGAACTATG TGTGGAATGT GCTACTTCAT CGAGGTGCCA CCCCAGAGGG CATATTCCTT
    CGTGTGCCTG CTGGCAGCTA TGATCTTGAC CTCTTCACCA TGACGTGGGG CCCCACTATT
    GCTGCCCTTT CTTATGTCTT TGACAAAAGC CTTGAGGAAA CAATTATCCA GAAAGCCATC
    TCAGGCTTCA GGAAGTGCGC CATGATCTCC GCCCACTATG GCCTCAGCGA TGTGTTTGAC
    AATCTCATCA TATCTCTGTG CAAATTCACA GCTCTTAGCA GTGAGTCTAT TGAGAATCTG
    CCCAGTGTGT TTGGAAGCAA CCCCAAAGCC CACATTGCAG CCAAGACAGT ATTCCATTTG
    GCCCATCGTC ATGGTGATAT CCTGCGGGAG GGCTGGAAGA ATATCATGGA AGCCATGCTG
    CAGCTCTTCC GAGCCCAACT GCTTCCCAAA GCTATGGTGG AGGTAGAAGA TTTTGTGGAT
    CCCAATGGCA AGATCTCTCT ACAGAGGGAG GAGACACCAT CAAACCGAGG AGAGTCGACA
    GTGCTAAGCT TTGTGAGCTG GCTAACACTA AGTGGTACTG AGCAGTCTAG TGTGCGGGGC
    CCATCCACTG AGAACCAAGA GGCCAAAAGA GCGGCCTTGG ACTGCATCAA GCATTGTGAC
    CCAGAAAAGA TGATCACAGA AAGCAAGTTC CTTCAGCTGG AGTCACTGCA GGAGCTCATG
    AAGGCTCTGG TCTCAGTGAC GCCAGATGAA GAGACATATG ATGAGGAGGA TGCTGCTTTC
    TGCCTAGAGA TGCTGCTGAG GATTGTGCTG GAGAACAGGG ACCGTGTGGG TTGTGTGTGG
    CAGACTGTCC GAGACCATCT ATATCACCTG TGTGTCCAGG CACAGGATTT CTGCTTCCTT
    GTGGAGCGGG CAGTGGTGGG GCTGCTCCGC TTGGCCATTC GACTACTCCG AAGAGAAGAG
    ATCAGTGGCC AGGTGCTGCT CTCCCTGCGT ATTTTGCTAC TAATGAAGCC CAGCGTGCTG
    TCCCGAGTCA GTCACCAGGT AGCCTATGGG CTCCATGAAC TTCTTAAGAC CAACGCAGCC
    AACATCCACT CAGGTGACGA CTGGGCCACT CTCTTCACAC TGCTGGAATG CATTGGCTCA
    GGTGTAAAGC CTCCAGCTGC CCTGCAGGCC ACAGCCAGGG CTGAAGCACC TGATGCCGGG
    GCCCAATCAG ACAGCGAACT CCCATCCTAC CATCAGAATG ATGTGAGCCT GGACCGAGGA
    TACACTTCTG ACTCGGAGGT CTATGCTGAC CATTGCAGGC CTGGCAAGAT CCACCGATCA
    GCCACTGATG CTGATGTAGT CAACAGCGGC TGGTTAGTGG TGGGGAAGGA TGACATTGAT
    AACTCCAAGC CAGGTCCTGG GCCCAGCCGG CTAGGCCCTT CACCCCTGGT CAATCAGTAC
    AGCCTAACAG TGGGGCTGGA CCTCGGGCCA CACGACACTA AGTCCCTGCT TAAATGTGTA
    GAATCTCTGT CCTTCATTGT GCGTGATGCT GCCCACATTA CACCTGACAA CTTTGAGCTC
    TGCGTGAAGA CTCTCCGTAT CTTTGTGGAG GCCAGTCTGA ATGGCGGGTG CAAGTCCCAG
    GAGAAACGTG GCAAGAGTCA CAAATATGAC AGCAAAGGAA ACCGCTTCAA GAAGAAATCC
    AAGGAAGGTT CAGTGCTTCG GCGGCCTCGA ACCTCTGGCC AACATGCCAC TCGGGGTGGG
    CATAGTGACG ATGATGAGGA TGAAGGGGTG CCTGCCAGCT ACCATACGGT GTCTTTACAG
    GTCAGTCAGG ACTTGTTAGA TCTGATGCAC ACCCTGCACA CGCGAGCAGC CTCTATCTAC
    AGCTCTTGGG CGGAGGAACA GCGCCACCTA GAGACAGGTG GCCGGAAGAT AGAAGCGGAT
    TCACGTACCC TCTGGGCCCA CTGCTGGTGT CCCTTACTGC AGGGCATTGC CTGCTTGTGC
    TGTGATGCCC GGCGCCAGGT GCGGATGCAA GCACTGACTT ATCTGCAACG AGCACTACTG
    GTACATGATC TGCAGAAGCT AGATGCCCTG GAGTGGGAGT CCTGTTTTAA CAAGGTGCTG
    TTTCCTCTAC TGACCAAACT CTTGGAGAAC ATCAGCCCTG CAGATGTGGG TGGGATGGAG
    GAGACTCGAA TGAGGGCTTC CACACTGCTG TCTAAGGTCT TCCTGCAGCA CCTGTCTCCA
    CTGCTGTCAC TCTCTACCTT TGCGGCCCTC TGGCTGACCA TCCTGGACTT CATGGACAAG
    TATATGCATG CAGGATCCAG TGACTTACTG TCAGAGGCCA TCCCTGAGTC TCTGAAGAAC
    ATGCTCCTGG TGATGGACAC AGCAGAGATT TTCCACAATG CAGATGCCCG AGGAGGCAGC
    CCCTCAGCCC TCTGGGAGAT CACCTGGGAG CGCATTGACT GTTTTCTGCC CCACCTACGA
    GATGAGCTCT TCAAGCAGAC TGTCATCCAG GACCCCATGC CACTGGAGCC ACATGCCCAA
    AAAACTCTGG CCTCAGCCCA CATGACTCCT GCTGCTGGAG ACACAAGGAC ACCTGGCCAT
    CCACCTCCCC CAGAGATGCC TTCTGAGCTG GGGGCCTGTG ACTTCGAGAA GCCTGAGGGC
    CCTCGACCTG CCAACAGCAG CTCTCCTGGA TCACCAGTGG CATCTAGCCC CAACAGACTG
    AGCCCTACTT CAGATGGGCC TCCTCCTCTG GCTCAGCCTC CACTGATCCT GCAGCCCTTG
    GCCTCCCCAT TGCAGGTGGG CGTACCACCC ATGACTCTAC CAATCATCCT CAACCCTGCT
    CTCATTGAGG CCACTTCACC AGTGCCCCTC CTGGCCACAC CCCGCCCCAC AGACCCAATG
    CCCACCTCCG AGGTCAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27950
    Clone ID Related Accession (Same CDS sequence) XM_005637660.3 , XM_005637660.2
    Accession Version XM_005637660.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5661bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726109.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005637660.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
    AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA
    CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT
    CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGA TTTCTCCATG TCTCAACAAA
    CTGGAGCTAA GTAGTGGGGA GCAGACCAAA GCCCTGAACC AGTTAGAGAG GCTGAAAATG
    AGAGCAGGAG GCATGAGTGA TTCATCCAAG TGGAAGAAAC AGAAGAGATC CCCCCGGCCC
    CTGCGCCATA TGACCAAAGT AACATCAGGT TCAGAGTTGC CCACCTCCAA TGGAACCACC
    TTATCCTCTA ACCTCACTGG GGGCATGCCC TTCATTGATG TGCCCACTCC CATCTCCTCT
    GCAAGTTCAG AAGCTGCCTC AACGGTGGTC AGCCCCTCTA CAGACAGTGG CCTGGAGTTC
    TCCTCTCAGA CCACCTCCAA GGAGGACCTC ACTGACCTGG AGCAACCTGG CTCTCCAGGG
    TATAGCACAG CTACAGAGCC TGGTAGCAGT GAGCTAGGGG TTCCTGAGCA GCCTGACCCG
    CAGCAGGAAG GAGTCCATGT GGAAAAGGCC CAGTCAGCAT CTGTGGAATC CATCCCCGAA
    GTGTTAGAGG AGTGCACGTC TCCTGCTGAC CACTCTGACT CTGCCTCTGT CCATGACATG
    GATTACGTCA ATCCCCGGGG TGTGCGCTTT ACACAGTCCT CCCAGAAAGA AGGTACAGCT
    TTGGTTCCCT ATGGTCTTCC CTGCATCCGT GAGCTCTTCC GCTTCCTCAT CTCCCTCACC
    AATCCACATG ACCGCCACAA TTCAGAAGTT ATGATCCACA TGGGACTGCA TTTGCTGACA
    GTGGCTCTTG AATCTGCCCC TGTAGCCCAG TGCCAAACCC TTTTGGGCCT CATCAAAGAT
    GAGATGTGCC GCCACTTACT CCAGCTACTC AGTGTAGAGC GACTCAACCT TTATGCTGCT
    TCCCTGCGGG TATGCTTCCT GCTTTTTGAG AGCATGAGGG AACATCTCAA GTTCCAATTG
    GAGATGTACA TCAAAAAGCT CATGGAGATC ATCACTGTGG AGAACCCCAA AATGCCTTAT
    GAGATGAAAG AGATGGCACT GGAGGCCATT GTGCAGCTCT GGCACATCCC CAGTTTTGTC
    ACTGAGCTCT ATATCAACTA TGATTGTGAC TACTACTGTT CAAACCTCTT TGAAGACCTC
    ACCAAGCTGC TGTCCAAGAA TGCCTTCCCT GTGTCTGGTC AGCTCTATAC AACACACCTC
    CTGTCTCTTG ATGCCCTGTT GACAGTGATT GACAGCACTG AGGCCCATTG CCAGGCCAAA
    GTCCTCAACA GTCTTACCCA GCAAGAGAAG AAAGAAGCAG CCAGATCTGG CTATGAGGCG
    GTGGATGGTA CCCGAGAATC CAGCAATAGT GAGAGAGCAG CCAGCGATGG GAAACCTGTA
    GGCATAGCCG CAGACATCCC AGGTCTACAT CTGCCAGGTG GAGGGCGGCT GCCAGCAGAA
    CATGGGAAGC CAGGATGCAG TGATCTGGAG GAAGCTGCTG ACTCTGGGGC TGACAAAAAG
    TTTACCCGGA AGCCACCTCG ATTTTCCTGT CTCCTGCCAG ATCCACGGGA ACTGATTGAA
    ATTAAAAACA AAAAGAAGCT GCTAATCACT GGCACAGAGC AGTTCAACCA GAAACCAAAG
    AAGGGAATCC AGTTTCTGCA GGAGAAAGGC CTCCTCACCA TCCCAATGGA CAACACAGAG
    GTAGCCCAGT GGCTCCGAGA GAACCCTCGG CTGGACAAGA AAATGATTGG AGAGTTTGTG
    AGTGACCGCA AAAACATTGA CCTGTTGGAG AGCTTTGTGA GCACCTTCAG CTTTCAGGGT
    CTGCGGCTGG ATGAAGCTCT TCGTCTCTAC CTGGAAGCCT TTCGCTTACC TGGGGAAGCA
    CCAGTCATCC AGAGGTTGCT GGAGGCATTC ACAGAGCATT GGAGGAATTG TAATGGCTCC
    CCATTTGCCA ATAGCGATGC CTGCTTTGCT CTGGCCTATG CTGTCATCTT GCTTAATACT
    GACCAGCACA ACCACAACGT TCGCAAACAG AATGCACCCA TGACTCTAGA GGAGTTTCGC
    AAAAACCTAA AAGGTGTGAA TGGAGGCAAG GACTTTGAGC AAGACATCCT GGAGGACATG
    TACCATGCCA TCAAGAATGA GGAAATTGTG ATGCCTGAAG AGCAGACAGG CTTGGTTCGG
    GAGAACTATG TGTGGAATGT GCTACTTCAT CGAGGTGCCA CCCCAGAGGG CATATTCCTT
    CGTGTGCCTG CTGGCAGCTA TGATCTTGAC CTCTTCACCA TGACGTGGGG CCCCACTATT
    GCTGCCCTTT CTTATGTCTT TGACAAAAGC CTTGAGGAAA CAATTATCCA GAAAGCCATC
    TCAGGCTTCA GGAAGTGCGC CATGATCTCC GCCCACTATG GCCTCAGCGA TGTGTTTGAC
    AATCTCATCA TATCTCTGTG CAAATTCACA GCTCTTAGCA GTGAGTCTAT TGAGAATCTG
    CCCAGTGTGT TTGGAAGCAA CCCCAAAGCC CACATTGCAG CCAAGACAGT ATTCCATTTG
    GCCCATCGTC ATGGTGATAT CCTGCGGGAG GGCTGGAAGA ATATCATGGA AGCCATGCTG
    CAGCTCTTCC GAGCCCAACT GCTTCCCAAA GCTATGGTGG AGGTAGAAGA TTTTGTGGAT
    CCCAATGGCA AGATCTCTCT ACAGAGGGAG GAGACACCAT CAAACCGAGG AGAGTCGACA
    GTGCTAAGCT TTGTGAGCTG GCTAACACTA AGTGGTACTG AGCAGTCTAG TGTGCGGGGC
    CCATCCACTG AGAACCAAGA GGCCAAAAGA GCGGCCTTGG ACTGCATCAA GCATTGTGAC
    CCAGAAAAGA TGATCACAGA AAGCAAGTTC CTTCAGCTGG AGTCACTGCA GGAGCTCATG
    AAGGCTCTGG TCTCAGTGAC GCCAGATGAA GAGACATATG ATGAGGAGGA TGCTGCTTTC
    TGCCTAGAGA TGCTGCTGAG GATTGTGCTG GAGAACAGGG ACCGTGTGGG TTGTGTGTGG
    CAGACTGTCC GAGACCATCT ATATCACCTG TGTGTCCAGG CACAGGATTT CTGCTTCCTT
    GTGGAGCGGG CAGTGGTGGG GCTGCTCCGC TTGGCCATTC GACTACTCCG AAGAGAAGAG
    ATCAGTGGCC AGGTGCTGCT CTCCCTGCGT ATTTTGCTAC TAATGAAGCC CAGCGTGCTG
    TCCCGAGTCA GTCACCAGGT AGCCTATGGG CTCCATGAAC TTCTTAAGAC CAACGCAGCC
    AACATCCACT CAGGTGACGA CTGGGCCACT CTCTTCACAC TGCTGGAATG CATTGGCTCA
    GGTGTAAAGC CTCCAGCTGC CCTGCAGGCC ACAGCCAGGG CTGAAGCACC TGATGCCGGG
    GCCCAATCAG ACAGCGAACT CCCATCCTAC CATCAGAATG ATGTGAGCCT GGACCGAGGA
    TACACTTCTG ACTCGGAGGT CTATGCTGAC CATTGCAGGC CTGGCAAGAT CCACCGATCA
    GCCACTGATG CTGATGTAGT CAACAGCGGC TGGTTAGTGG TGGGGAAGGA TGACATTGAT
    AACTCCAAGC CAGGTCCTGG GCCCAGCCGG CTAGGCCCTT CACCCCTGGT CAATCAGTAC
    AGCCTAACAG TGGGGCTGGA CCTCGGGCCA CACGACACTA AGTCCCTGCT TAAATGTGTA
    GAATCTCTGT CCTTCATTGT GCGTGATGCT GCCCACATTA CACCTGACAA CTTTGAGCTC
    TGCGTGAAGA CTCTCCGTAT CTTTGTGGAG GCCAGTCTGA ATGGCGGGTG CAAGTCCCAG
    GAGAAACGTG GCAAGAGTCA CAAATATGAC AGCAAAGGAA ACCGCTTCAA GAAGAAATCC
    AAGGAAGGTT CAGTGCTTCG GCGGCCTCGA ACCTCTGGCC AACATGCCAC TCGGGGTGGG
    CATAGTGACG ATGATGAGGA TGAAGGGGTG CCTGCCAGCT ACCATACGGT GTCTTTACAG
    GTCAGTCAGG ACTTGTTAGA TCTGATGCAC ACCCTGCACA CGCGAGCAGC CTCTATCTAC
    AGCTCTTGGG CGGAGGAACA GCGCCACCTA GAGACAGGTG GCCGGAAGAT AGAAGCGGAT
    TCACGTACCC TCTGGGCCCA CTGCTGGTGT CCCTTACTGC AGGGCATTGC CTGCTTGTGC
    TGTGATGCCC GGCGCCAGGT GCGGATGCAA GCACTGACTT ATCTGCAACG AGCACTACTG
    GTACATGATC TGCAGAAGCT AGATGCCCTG GAGTGGGAGT CCTGTTTTAA CAAGGTGCTG
    TTTCCTCTAC TGACCAAACT CTTGGAGAAC ATCAGCCCTG CAGATGTGGG TGGGATGGAG
    GAGACTCGAA TGAGGGCTTC CACACTGCTG TCTAAGGTCT TCCTGCAGCA CCTGTCTCCA
    CTGCTGTCAC TCTCTACCTT TGCGGCCCTC TGGCTGACCA TCCTGGACTT CATGGACAAG
    TATATGCATG CAGGATCCAG TGACTTACTG TCAGAGGCCA TCCCTGAGTC TCTGAAGAAC
    ATGCTCCTGG TGATGGACAC AGCAGAGATT TTCCACAATG CAGATGCCCG AGGAGGCAGC
    CCCTCAGCCC TCTGGGAGAT CACCTGGGAG CGCATTGACT GTTTTCTGCC CCACCTACGA
    GATGAGCTCT TCAAGCAGAC TGTCATCCAG GACCCCATGC CACTGGAGCC ACATGCCCAA
    AAAACTCTGG CCTCAGCCCA CATGACTCCT GCTGCTGGAG ACACAAGGAC ACCTGGCCAT
    CCACCTCCCC CAGAGATGCC TTCTGAGCTG GGGGCCTGTG ACTTCGAGAA GCCTGAGGGC
    CCTCGACCTG CCAACAGCAG CTCTCCTGGA TCACCAGTGG CATCTAGCCC CAACAGACTG
    AGCCCTACTT CAGATGGGCC TCCTCCTCTG GCTCAGCCTC CACTGATCCT GCAGCCCTTG
    GCCTCCCCAT TGCAGGTGGG CGTACCACCC ATGACTCTAC CAATCATCCT CAACCCTGCT
    CTCATTGAGG CCACTTCACC AGTGCCCCTC CTGGCCACAC CCCGCCCCAC AGACCCAATG
    CCCACCTCCG AGGTCAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005637717.1
    CDS251..5911
    Translation

    Target ORF information:

    RefSeq Version XM_005637660.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005637660.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
    AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA
    CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT
    CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGA TTTCTCCATG TCTCAACAAA
    CTGGAGCTAA GTAGTGGGGA GCAGACCAAA GCCCTGAACC AGTTAGAGAG GCTGAAAATG
    AGAGCAGGAG GCATGAGTGA TTCATCCAAG TGGAAGAAAC AGAAGAGATC CCCCCGGCCC
    CTGCGCCATA TGACCAAAGT AACATCAGGT TCAGAGTTGC CCACCTCCAA TGGAACCACC
    TTATCCTCTA ACCTCACTGG GGGCATGCCC TTCATTGATG TGCCCACTCC CATCTCCTCT
    GCAAGTTCAG AAGCTGCCTC AACGGTGGTC AGCCCCTCTA CAGACAGTGG CCTGGAGTTC
    TCCTCTCAGA CCACCTCCAA GGAGGACCTC ACTGACCTGG AGCAACCTGG CTCTCCAGGG
    TATAGCACAG CTACAGAGCC TGGTAGCAGT GAGCTAGGGG TTCCTGAGCA GCCTGACCCG
    CAGCAGGAAG GAGTCCATGT GGAAAAGGCC CAGTCAGCAT CTGTGGAATC CATCCCCGAA
    GTGTTAGAGG AGTGCACGTC TCCTGCTGAC CACTCTGACT CTGCCTCTGT CCATGACATG
    GATTACGTCA ATCCCCGGGG TGTGCGCTTT ACACAGTCCT CCCAGAAAGA AGGTACAGCT
    TTGGTTCCCT ATGGTCTTCC CTGCATCCGT GAGCTCTTCC GCTTCCTCAT CTCCCTCACC
    AATCCACATG ACCGCCACAA TTCAGAAGTT ATGATCCACA TGGGACTGCA TTTGCTGACA
    GTGGCTCTTG AATCTGCCCC TGTAGCCCAG TGCCAAACCC TTTTGGGCCT CATCAAAGAT
    GAGATGTGCC GCCACTTACT CCAGCTACTC AGTGTAGAGC GACTCAACCT TTATGCTGCT
    TCCCTGCGGG TATGCTTCCT GCTTTTTGAG AGCATGAGGG AACATCTCAA GTTCCAATTG
    GAGATGTACA TCAAAAAGCT CATGGAGATC ATCACTGTGG AGAACCCCAA AATGCCTTAT
    GAGATGAAAG AGATGGCACT GGAGGCCATT GTGCAGCTCT GGCACATCCC CAGTTTTGTC
    ACTGAGCTCT ATATCAACTA TGATTGTGAC TACTACTGTT CAAACCTCTT TGAAGACCTC
    ACCAAGCTGC TGTCCAAGAA TGCCTTCCCT GTGTCTGGTC AGCTCTATAC AACACACCTC
    CTGTCTCTTG ATGCCCTGTT GACAGTGATT GACAGCACTG AGGCCCATTG CCAGGCCAAA
    GTCCTCAACA GTCTTACCCA GCAAGAGAAG AAAGAAGCAG CCAGATCTGG CTATGAGGCG
    GTGGATGGTA CCCGAGAATC CAGCAATAGT GAGAGAGCAG CCAGCGATGG GAAACCTGTA
    GGCATAGCCG CAGACATCCC AGGTCTACAT CTGCCAGGTG GAGGGCGGCT GCCAGCAGAA
    CATGGGAAGC CAGGATGCAG TGATCTGGAG GAAGCTGCTG ACTCTGGGGC TGACAAAAAG
    TTTACCCGGA AGCCACCTCG ATTTTCCTGT CTCCTGCCAG ATCCACGGGA ACTGATTGAA
    ATTAAAAACA AAAAGAAGCT GCTAATCACT GGCACAGAGC AGTTCAACCA GAAACCAAAG
    AAGGGAATCC AGTTTCTGCA GGAGAAAGGC CTCCTCACCA TCCCAATGGA CAACACAGAG
    GTAGCCCAGT GGCTCCGAGA GAACCCTCGG CTGGACAAGA AAATGATTGG AGAGTTTGTG
    AGTGACCGCA AAAACATTGA CCTGTTGGAG AGCTTTGTGA GCACCTTCAG CTTTCAGGGT
    CTGCGGCTGG ATGAAGCTCT TCGTCTCTAC CTGGAAGCCT TTCGCTTACC TGGGGAAGCA
    CCAGTCATCC AGAGGTTGCT GGAGGCATTC ACAGAGCATT GGAGGAATTG TAATGGCTCC
    CCATTTGCCA ATAGCGATGC CTGCTTTGCT CTGGCCTATG CTGTCATCTT GCTTAATACT
    GACCAGCACA ACCACAACGT TCGCAAACAG AATGCACCCA TGACTCTAGA GGAGTTTCGC
    AAAAACCTAA AAGGTGTGAA TGGAGGCAAG GACTTTGAGC AAGACATCCT GGAGGACATG
    TACCATGCCA TCAAGAATGA GGAAATTGTG ATGCCTGAAG AGCAGACAGG CTTGGTTCGG
    GAGAACTATG TGTGGAATGT GCTACTTCAT CGAGGTGCCA CCCCAGAGGG CATATTCCTT
    CGTGTGCCTG CTGGCAGCTA TGATCTTGAC CTCTTCACCA TGACGTGGGG CCCCACTATT
    GCTGCCCTTT CTTATGTCTT TGACAAAAGC CTTGAGGAAA CAATTATCCA GAAAGCCATC
    TCAGGCTTCA GGAAGTGCGC CATGATCTCC GCCCACTATG GCCTCAGCGA TGTGTTTGAC
    AATCTCATCA TATCTCTGTG CAAATTCACA GCTCTTAGCA GTGAGTCTAT TGAGAATCTG
    CCCAGTGTGT TTGGAAGCAA CCCCAAAGCC CACATTGCAG CCAAGACAGT ATTCCATTTG
    GCCCATCGTC ATGGTGATAT CCTGCGGGAG GGCTGGAAGA ATATCATGGA AGCCATGCTG
    CAGCTCTTCC GAGCCCAACT GCTTCCCAAA GCTATGGTGG AGGTAGAAGA TTTTGTGGAT
    CCCAATGGCA AGATCTCTCT ACAGAGGGAG GAGACACCAT CAAACCGAGG AGAGTCGACA
    GTGCTAAGCT TTGTGAGCTG GCTAACACTA AGTGGTACTG AGCAGTCTAG TGTGCGGGGC
    CCATCCACTG AGAACCAAGA GGCCAAAAGA GCGGCCTTGG ACTGCATCAA GCATTGTGAC
    CCAGAAAAGA TGATCACAGA AAGCAAGTTC CTTCAGCTGG AGTCACTGCA GGAGCTCATG
    AAGGCTCTGG TCTCAGTGAC GCCAGATGAA GAGACATATG ATGAGGAGGA TGCTGCTTTC
    TGCCTAGAGA TGCTGCTGAG GATTGTGCTG GAGAACAGGG ACCGTGTGGG TTGTGTGTGG
    CAGACTGTCC GAGACCATCT ATATCACCTG TGTGTCCAGG CACAGGATTT CTGCTTCCTT
    GTGGAGCGGG CAGTGGTGGG GCTGCTCCGC TTGGCCATTC GACTACTCCG AAGAGAAGAG
    ATCAGTGGCC AGGTGCTGCT CTCCCTGCGT ATTTTGCTAC TAATGAAGCC CAGCGTGCTG
    TCCCGAGTCA GTCACCAGGT AGCCTATGGG CTCCATGAAC TTCTTAAGAC CAACGCAGCC
    AACATCCACT CAGGTGACGA CTGGGCCACT CTCTTCACAC TGCTGGAATG CATTGGCTCA
    GGTGTAAAGC CTCCAGCTGC CCTGCAGGCC ACAGCCAGGG CTGAAGCACC TGATGCCGGG
    GCCCAATCAG ACAGCGAACT CCCATCCTAC CATCAGAATG ATGTGAGCCT GGACCGAGGA
    TACACTTCTG ACTCGGAGGT CTATGCTGAC CATTGCAGGC CTGGCAAGAT CCACCGATCA
    GCCACTGATG CTGATGTAGT CAACAGCGGC TGGTTAGTGG TGGGGAAGGA TGACATTGAT
    AACTCCAAGC CAGGTCCTGG GCCCAGCCGG CTAGGCCCTT CACCCCTGGT CAATCAGTAC
    AGCCTAACAG TGGGGCTGGA CCTCGGGCCA CACGACACTA AGTCCCTGCT TAAATGTGTA
    GAATCTCTGT CCTTCATTGT GCGTGATGCT GCCCACATTA CACCTGACAA CTTTGAGCTC
    TGCGTGAAGA CTCTCCGTAT CTTTGTGGAG GCCAGTCTGA ATGGCGGGTG CAAGTCCCAG
    GAGAAACGTG GCAAGAGTCA CAAATATGAC AGCAAAGGAA ACCGCTTCAA GAAGAAATCC
    AAGGAAGGTT CAGTGCTTCG GCGGCCTCGA ACCTCTGGCC AACATGCCAC TCGGGGTGGG
    CATAGTGACG ATGATGAGGA TGAAGGGGTG CCTGCCAGCT ACCATACGGT GTCTTTACAG
    GTCAGTCAGG ACTTGTTAGA TCTGATGCAC ACCCTGCACA CGCGAGCAGC CTCTATCTAC
    AGCTCTTGGG CGGAGGAACA GCGCCACCTA GAGACAGGTG GCCGGAAGAT AGAAGCGGAT
    TCACGTACCC TCTGGGCCCA CTGCTGGTGT CCCTTACTGC AGGGCATTGC CTGCTTGTGC
    TGTGATGCCC GGCGCCAGGT GCGGATGCAA GCACTGACTT ATCTGCAACG AGCACTACTG
    GTACATGATC TGCAGAAGCT AGATGCCCTG GAGTGGGAGT CCTGTTTTAA CAAGGTGCTG
    TTTCCTCTAC TGACCAAACT CTTGGAGAAC ATCAGCCCTG CAGATGTGGG TGGGATGGAG
    GAGACTCGAA TGAGGGCTTC CACACTGCTG TCTAAGGTCT TCCTGCAGCA CCTGTCTCCA
    CTGCTGTCAC TCTCTACCTT TGCGGCCCTC TGGCTGACCA TCCTGGACTT CATGGACAAG
    TATATGCATG CAGGATCCAG TGACTTACTG TCAGAGGCCA TCCCTGAGTC TCTGAAGAAC
    ATGCTCCTGG TGATGGACAC AGCAGAGATT TTCCACAATG CAGATGCCCG AGGAGGCAGC
    CCCTCAGCCC TCTGGGAGAT CACCTGGGAG CGCATTGACT GTTTTCTGCC CCACCTACGA
    GATGAGCTCT TCAAGCAGAC TGTCATCCAG GACCCCATGC CACTGGAGCC ACATGCCCAA
    AAAACTCTGG CCTCAGCCCA CATGACTCCT GCTGCTGGAG ACACAAGGAC ACCTGGCCAT
    CCACCTCCCC CAGAGATGCC TTCTGAGCTG GGGGCCTGTG ACTTCGAGAA GCCTGAGGGC
    CCTCGACCTG CCAACAGCAG CTCTCCTGGA TCACCAGTGG CATCTAGCCC CAACAGACTG
    AGCCCTACTT CAGATGGGCC TCCTCCTCTG GCTCAGCCTC CACTGATCCT GCAGCCCTTG
    GCCTCCCCAT TGCAGGTGGG CGTACCACCC ATGACTCTAC CAATCATCCT CAACCCTGCT
    CTCATTGAGG CCACTTCACC AGTGCCCCTC CTGGCCACAC CCCGCCCCAC AGACCCAATG
    CCCACCTCCG AGGTCAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27951
    Clone ID Related Accession (Same CDS sequence) XM_005637661.3 , XM_005637661.2
    Accession Version XM_005637661.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5589bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006610.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005637661.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
    AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA
    CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT
    CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC
    ATGAGTGATT CATCCAAGTG GAAGAAACAG AAGAGATCCC CCCGGCCCCT GCGCCATATG
    ACCAAAGTAA CATCAGGTTC AGAGTTGCCC ACCTCCAATG GAACCACCTT ATCCTCTAAC
    CTCACTGGGG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAA CGGTGGTCAG CCCCTCTACA GACAGTGGCC TGGAGTTCTC CTCTCAGACC
    ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAACCTGGCT CTCCAGGGTA TAGCACAGCT
    ACAGAGCCTG GTAGCAGTGA GCTAGGGGTT CCTGAGCAGC CTGACCCGCA GCAGGAAGGA
    GTCCATGTGG AAAAGGCCCA GTCAGCATCT GTGGAATCCA TCCCCGAAGT GTTAGAGGAG
    TGCACGTCTC CTGCTGACCA CTCTGACTCT GCCTCTGTCC ATGACATGGA TTACGTCAAT
    CCCCGGGGTG TGCGCTTTAC ACAGTCCTCC CAGAAAGAAG GTACAGCTTT GGTTCCCTAT
    GGTCTTCCCT GCATCCGTGA GCTCTTCCGC TTCCTCATCT CCCTCACCAA TCCACATGAC
    CGCCACAATT CAGAAGTTAT GATCCACATG GGACTGCATT TGCTGACAGT GGCTCTTGAA
    TCTGCCCCTG TAGCCCAGTG CCAAACCCTT TTGGGCCTCA TCAAAGATGA GATGTGCCGC
    CACTTACTCC AGCTACTCAG TGTAGAGCGA CTCAACCTTT ATGCTGCTTC CCTGCGGGTA
    TGCTTCCTGC TTTTTGAGAG CATGAGGGAA CATCTCAAGT TCCAATTGGA GATGTACATC
    AAAAAGCTCA TGGAGATCAT CACTGTGGAG AACCCCAAAA TGCCTTATGA GATGAAAGAG
    ATGGCACTGG AGGCCATTGT GCAGCTCTGG CACATCCCCA GTTTTGTCAC TGAGCTCTAT
    ATCAACTATG ATTGTGACTA CTACTGTTCA AACCTCTTTG AAGACCTCAC CAAGCTGCTG
    TCCAAGAATG CCTTCCCTGT GTCTGGTCAG CTCTATACAA CACACCTCCT GTCTCTTGAT
    GCCCTGTTGA CAGTGATTGA CAGCACTGAG GCCCATTGCC AGGCCAAAGT CCTCAACAGT
    CTTACCCAGC AAGAGAAGAA AGAAGCAGCC AGATCTGGCT ATGAGGCGGT GGATGGTACC
    CGAGAATCCA GCAATAGTGA GAGAGCAGCC AGCGATGGGA AACCTGTAGG CATAGCCGCA
    GACATCCCAG GTCTACATCT GCCAGGTGGA GGGCGGCTGC CAGCAGAACA TGGGAAGCCA
    GGATGCAGTG ATCTGGAGGA AGCTGCTGAC TCTGGGGCTG ACAAAAAGTT TACCCGGAAG
    CCACCTCGAT TTTCCTGTCT CCTGCCAGAT CCACGGGAAC TGATTGAAAT TAAAAACAAA
    AAGAAGCTGC TAATCACTGG CACAGAGCAG TTCAACCAGA AACCAAAGAA GGGAATCCAG
    TTTCTGCAGG AGAAAGGCCT CCTCACCATC CCAATGGACA ACACAGAGGT AGCCCAGTGG
    CTCCGAGAGA ACCCTCGGCT GGACAAGAAA ATGATTGGAG AGTTTGTGAG TGACCGCAAA
    AACATTGACC TGTTGGAGAG CTTTGTGAGC ACCTTCAGCT TTCAGGGTCT GCGGCTGGAT
    GAAGCTCTTC GTCTCTACCT GGAAGCCTTT CGCTTACCTG GGGAAGCACC AGTCATCCAG
    AGGTTGCTGG AGGCATTCAC AGAGCATTGG AGGAATTGTA ATGGCTCCCC ATTTGCCAAT
    AGCGATGCCT GCTTTGCTCT GGCCTATGCT GTCATCTTGC TTAATACTGA CCAGCACAAC
    CACAACGTTC GCAAACAGAA TGCACCCATG ACTCTAGAGG AGTTTCGCAA AAACCTAAAA
    GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AGGACATGTA CCATGCCATC
    AAGAATGAGG AAATTGTGAT GCCTGAAGAG CAGACAGGCT TGGTTCGGGA GAACTATGTG
    TGGAATGTGC TACTTCATCG AGGTGCCACC CCAGAGGGCA TATTCCTTCG TGTGCCTGCT
    GGCAGCTATG ATCTTGACCT CTTCACCATG ACGTGGGGCC CCACTATTGC TGCCCTTTCT
    TATGTCTTTG ACAAAAGCCT TGAGGAAACA ATTATCCAGA AAGCCATCTC AGGCTTCAGG
    AAGTGCGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATA
    TCTCTGTGCA AATTCACAGC TCTTAGCAGT GAGTCTATTG AGAATCTGCC CAGTGTGTTT
    GGAAGCAACC CCAAAGCCCA CATTGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT
    GGTGATATCC TGCGGGAGGG CTGGAAGAAT ATCATGGAAG CCATGCTGCA GCTCTTCCGA
    GCCCAACTGC TTCCCAAAGC TATGGTGGAG GTAGAAGATT TTGTGGATCC CAATGGCAAG
    ATCTCTCTAC AGAGGGAGGA GACACCATCA AACCGAGGAG AGTCGACAGT GCTAAGCTTT
    GTGAGCTGGC TAACACTAAG TGGTACTGAG CAGTCTAGTG TGCGGGGCCC ATCCACTGAG
    AACCAAGAGG CCAAAAGAGC GGCCTTGGAC TGCATCAAGC ATTGTGACCC AGAAAAGATG
    ATCACAGAAA GCAAGTTCCT TCAGCTGGAG TCACTGCAGG AGCTCATGAA GGCTCTGGTC
    TCAGTGACGC CAGATGAAGA GACATATGAT GAGGAGGATG CTGCTTTCTG CCTAGAGATG
    CTGCTGAGGA TTGTGCTGGA GAACAGGGAC CGTGTGGGTT GTGTGTGGCA GACTGTCCGA
    GACCATCTAT ATCACCTGTG TGTCCAGGCA CAGGATTTCT GCTTCCTTGT GGAGCGGGCA
    GTGGTGGGGC TGCTCCGCTT GGCCATTCGA CTACTCCGAA GAGAAGAGAT CAGTGGCCAG
    GTGCTGCTCT CCCTGCGTAT TTTGCTACTA ATGAAGCCCA GCGTGCTGTC CCGAGTCAGT
    CACCAGGTAG CCTATGGGCT CCATGAACTT CTTAAGACCA ACGCAGCCAA CATCCACTCA
    GGTGACGACT GGGCCACTCT CTTCACACTG CTGGAATGCA TTGGCTCAGG TGTAAAGCCT
    CCAGCTGCCC TGCAGGCCAC AGCCAGGGCT GAAGCACCTG ATGCCGGGGC CCAATCAGAC
    AGCGAACTCC CATCCTACCA TCAGAATGAT GTGAGCCTGG ACCGAGGATA CACTTCTGAC
    TCGGAGGTCT ATGCTGACCA TTGCAGGCCT GGCAAGATCC ACCGATCAGC CACTGATGCT
    GATGTAGTCA ACAGCGGCTG GTTAGTGGTG GGGAAGGATG ACATTGATAA CTCCAAGCCA
    GGTCCTGGGC CCAGCCGGCT AGGCCCTTCA CCCCTGGTCA ATCAGTACAG CCTAACAGTG
    GGGCTGGACC TCGGGCCACA CGACACTAAG TCCCTGCTTA AATGTGTAGA ATCTCTGTCC
    TTCATTGTGC GTGATGCTGC CCACATTACA CCTGACAACT TTGAGCTCTG CGTGAAGACT
    CTCCGTATCT TTGTGGAGGC CAGTCTGAAT GGCGGGTGCA AGTCCCAGGA GAAACGTGGC
    AAGAGTCACA AATATGACAG CAAAGGAAAC CGCTTCAAGA AGAAATCCAA GGAAGGTTCA
    GTGCTTCGGC GGCCTCGAAC CTCTGGCCAA CATGCCACTC GGGGTGGGCA TAGTGACGAT
    GATGAGGATG AAGGGGTGCC TGCCAGCTAC CATACGGTGT CTTTACAGGT CAGTCAGGAC
    TTGTTAGATC TGATGCACAC CCTGCACACG CGAGCAGCCT CTATCTACAG CTCTTGGGCG
    GAGGAACAGC GCCACCTAGA GACAGGTGGC CGGAAGATAG AAGCGGATTC ACGTACCCTC
    TGGGCCCACT GCTGGTGTCC CTTACTGCAG GGCATTGCCT GCTTGTGCTG TGATGCCCGG
    CGCCAGGTGC GGATGCAAGC ACTGACTTAT CTGCAACGAG CACTACTGGT ACATGATCTG
    CAGAAGCTAG ATGCCCTGGA GTGGGAGTCC TGTTTTAACA AGGTGCTGTT TCCTCTACTG
    ACCAAACTCT TGGAGAACAT CAGCCCTGCA GATGTGGGTG GGATGGAGGA GACTCGAATG
    AGGGCTTCCA CACTGCTGTC TAAGGTCTTC CTGCAGCACC TGTCTCCACT GCTGTCACTC
    TCTACCTTTG CGGCCCTCTG GCTGACCATC CTGGACTTCA TGGACAAGTA TATGCATGCA
    GGATCCAGTG ACTTACTGTC AGAGGCCATC CCTGAGTCTC TGAAGAACAT GCTCCTGGTG
    ATGGACACAG CAGAGATTTT CCACAATGCA GATGCCCGAG GAGGCAGCCC CTCAGCCCTC
    TGGGAGATCA CCTGGGAGCG CATTGACTGT TTTCTGCCCC ACCTACGAGA TGAGCTCTTC
    AAGCAGACTG TCATCCAGGA CCCCATGCCA CTGGAGCCAC ATGCCCAAAA AACTCTGGCC
    TCAGCCCACA TGACTCCTGC TGCTGGAGAC ACAAGGACAC CTGGCCATCC ACCTCCCCCA
    GAGATGCCTT CTGAGCTGGG GGCCTGTGAC TTCGAGAAGC CTGAGGGCCC TCGACCTGCC
    AACAGCAGCT CTCCTGGATC ACCAGTGGCA TCTAGCCCCA ACAGACTGAG CCCTACTTCA
    GATGGGCCTC CTCCTCTGGC TCAGCCTCCA CTGATCCTGC AGCCCTTGGC CTCCCCATTG
    CAGGTGGGCG TACCACCCAT GACTCTACCA ATCATCCTCA ACCCTGCTCT CATTGAGGCC
    ACTTCACCAG TGCCCCTCCT GGCCACACCC CGCCCCACAG ACCCAATGCC CACCTCCGAG
    GTCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005637718.1
    CDS269..5857
    Translation

    Target ORF information:

    RefSeq Version XM_005637661.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005637661.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
    AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA
    CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT
    CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC
    ATGAGTGATT CATCCAAGTG GAAGAAACAG AAGAGATCCC CCCGGCCCCT GCGCCATATG
    ACCAAAGTAA CATCAGGTTC AGAGTTGCCC ACCTCCAATG GAACCACCTT ATCCTCTAAC
    CTCACTGGGG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAA CGGTGGTCAG CCCCTCTACA GACAGTGGCC TGGAGTTCTC CTCTCAGACC
    ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAACCTGGCT CTCCAGGGTA TAGCACAGCT
    ACAGAGCCTG GTAGCAGTGA GCTAGGGGTT CCTGAGCAGC CTGACCCGCA GCAGGAAGGA
    GTCCATGTGG AAAAGGCCCA GTCAGCATCT GTGGAATCCA TCCCCGAAGT GTTAGAGGAG
    TGCACGTCTC CTGCTGACCA CTCTGACTCT GCCTCTGTCC ATGACATGGA TTACGTCAAT
    CCCCGGGGTG TGCGCTTTAC ACAGTCCTCC CAGAAAGAAG GTACAGCTTT GGTTCCCTAT
    GGTCTTCCCT GCATCCGTGA GCTCTTCCGC TTCCTCATCT CCCTCACCAA TCCACATGAC
    CGCCACAATT CAGAAGTTAT GATCCACATG GGACTGCATT TGCTGACAGT GGCTCTTGAA
    TCTGCCCCTG TAGCCCAGTG CCAAACCCTT TTGGGCCTCA TCAAAGATGA GATGTGCCGC
    CACTTACTCC AGCTACTCAG TGTAGAGCGA CTCAACCTTT ATGCTGCTTC CCTGCGGGTA
    TGCTTCCTGC TTTTTGAGAG CATGAGGGAA CATCTCAAGT TCCAATTGGA GATGTACATC
    AAAAAGCTCA TGGAGATCAT CACTGTGGAG AACCCCAAAA TGCCTTATGA GATGAAAGAG
    ATGGCACTGG AGGCCATTGT GCAGCTCTGG CACATCCCCA GTTTTGTCAC TGAGCTCTAT
    ATCAACTATG ATTGTGACTA CTACTGTTCA AACCTCTTTG AAGACCTCAC CAAGCTGCTG
    TCCAAGAATG CCTTCCCTGT GTCTGGTCAG CTCTATACAA CACACCTCCT GTCTCTTGAT
    GCCCTGTTGA CAGTGATTGA CAGCACTGAG GCCCATTGCC AGGCCAAAGT CCTCAACAGT
    CTTACCCAGC AAGAGAAGAA AGAAGCAGCC AGATCTGGCT ATGAGGCGGT GGATGGTACC
    CGAGAATCCA GCAATAGTGA GAGAGCAGCC AGCGATGGGA AACCTGTAGG CATAGCCGCA
    GACATCCCAG GTCTACATCT GCCAGGTGGA GGGCGGCTGC CAGCAGAACA TGGGAAGCCA
    GGATGCAGTG ATCTGGAGGA AGCTGCTGAC TCTGGGGCTG ACAAAAAGTT TACCCGGAAG
    CCACCTCGAT TTTCCTGTCT CCTGCCAGAT CCACGGGAAC TGATTGAAAT TAAAAACAAA
    AAGAAGCTGC TAATCACTGG CACAGAGCAG TTCAACCAGA AACCAAAGAA GGGAATCCAG
    TTTCTGCAGG AGAAAGGCCT CCTCACCATC CCAATGGACA ACACAGAGGT AGCCCAGTGG
    CTCCGAGAGA ACCCTCGGCT GGACAAGAAA ATGATTGGAG AGTTTGTGAG TGACCGCAAA
    AACATTGACC TGTTGGAGAG CTTTGTGAGC ACCTTCAGCT TTCAGGGTCT GCGGCTGGAT
    GAAGCTCTTC GTCTCTACCT GGAAGCCTTT CGCTTACCTG GGGAAGCACC AGTCATCCAG
    AGGTTGCTGG AGGCATTCAC AGAGCATTGG AGGAATTGTA ATGGCTCCCC ATTTGCCAAT
    AGCGATGCCT GCTTTGCTCT GGCCTATGCT GTCATCTTGC TTAATACTGA CCAGCACAAC
    CACAACGTTC GCAAACAGAA TGCACCCATG ACTCTAGAGG AGTTTCGCAA AAACCTAAAA
    GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AGGACATGTA CCATGCCATC
    AAGAATGAGG AAATTGTGAT GCCTGAAGAG CAGACAGGCT TGGTTCGGGA GAACTATGTG
    TGGAATGTGC TACTTCATCG AGGTGCCACC CCAGAGGGCA TATTCCTTCG TGTGCCTGCT
    GGCAGCTATG ATCTTGACCT CTTCACCATG ACGTGGGGCC CCACTATTGC TGCCCTTTCT
    TATGTCTTTG ACAAAAGCCT TGAGGAAACA ATTATCCAGA AAGCCATCTC AGGCTTCAGG
    AAGTGCGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATA
    TCTCTGTGCA AATTCACAGC TCTTAGCAGT GAGTCTATTG AGAATCTGCC CAGTGTGTTT
    GGAAGCAACC CCAAAGCCCA CATTGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT
    GGTGATATCC TGCGGGAGGG CTGGAAGAAT ATCATGGAAG CCATGCTGCA GCTCTTCCGA
    GCCCAACTGC TTCCCAAAGC TATGGTGGAG GTAGAAGATT TTGTGGATCC CAATGGCAAG
    ATCTCTCTAC AGAGGGAGGA GACACCATCA AACCGAGGAG AGTCGACAGT GCTAAGCTTT
    GTGAGCTGGC TAACACTAAG TGGTACTGAG CAGTCTAGTG TGCGGGGCCC ATCCACTGAG
    AACCAAGAGG CCAAAAGAGC GGCCTTGGAC TGCATCAAGC ATTGTGACCC AGAAAAGATG
    ATCACAGAAA GCAAGTTCCT TCAGCTGGAG TCACTGCAGG AGCTCATGAA GGCTCTGGTC
    TCAGTGACGC CAGATGAAGA GACATATGAT GAGGAGGATG CTGCTTTCTG CCTAGAGATG
    CTGCTGAGGA TTGTGCTGGA GAACAGGGAC CGTGTGGGTT GTGTGTGGCA GACTGTCCGA
    GACCATCTAT ATCACCTGTG TGTCCAGGCA CAGGATTTCT GCTTCCTTGT GGAGCGGGCA
    GTGGTGGGGC TGCTCCGCTT GGCCATTCGA CTACTCCGAA GAGAAGAGAT CAGTGGCCAG
    GTGCTGCTCT CCCTGCGTAT TTTGCTACTA ATGAAGCCCA GCGTGCTGTC CCGAGTCAGT
    CACCAGGTAG CCTATGGGCT CCATGAACTT CTTAAGACCA ACGCAGCCAA CATCCACTCA
    GGTGACGACT GGGCCACTCT CTTCACACTG CTGGAATGCA TTGGCTCAGG TGTAAAGCCT
    CCAGCTGCCC TGCAGGCCAC AGCCAGGGCT GAAGCACCTG ATGCCGGGGC CCAATCAGAC
    AGCGAACTCC CATCCTACCA TCAGAATGAT GTGAGCCTGG ACCGAGGATA CACTTCTGAC
    TCGGAGGTCT ATGCTGACCA TTGCAGGCCT GGCAAGATCC ACCGATCAGC CACTGATGCT
    GATGTAGTCA ACAGCGGCTG GTTAGTGGTG GGGAAGGATG ACATTGATAA CTCCAAGCCA
    GGTCCTGGGC CCAGCCGGCT AGGCCCTTCA CCCCTGGTCA ATCAGTACAG CCTAACAGTG
    GGGCTGGACC TCGGGCCACA CGACACTAAG TCCCTGCTTA AATGTGTAGA ATCTCTGTCC
    TTCATTGTGC GTGATGCTGC CCACATTACA CCTGACAACT TTGAGCTCTG CGTGAAGACT
    CTCCGTATCT TTGTGGAGGC CAGTCTGAAT GGCGGGTGCA AGTCCCAGGA GAAACGTGGC
    AAGAGTCACA AATATGACAG CAAAGGAAAC CGCTTCAAGA AGAAATCCAA GGAAGGTTCA
    GTGCTTCGGC GGCCTCGAAC CTCTGGCCAA CATGCCACTC GGGGTGGGCA TAGTGACGAT
    GATGAGGATG AAGGGGTGCC TGCCAGCTAC CATACGGTGT CTTTACAGGT CAGTCAGGAC
    TTGTTAGATC TGATGCACAC CCTGCACACG CGAGCAGCCT CTATCTACAG CTCTTGGGCG
    GAGGAACAGC GCCACCTAGA GACAGGTGGC CGGAAGATAG AAGCGGATTC ACGTACCCTC
    TGGGCCCACT GCTGGTGTCC CTTACTGCAG GGCATTGCCT GCTTGTGCTG TGATGCCCGG
    CGCCAGGTGC GGATGCAAGC ACTGACTTAT CTGCAACGAG CACTACTGGT ACATGATCTG
    CAGAAGCTAG ATGCCCTGGA GTGGGAGTCC TGTTTTAACA AGGTGCTGTT TCCTCTACTG
    ACCAAACTCT TGGAGAACAT CAGCCCTGCA GATGTGGGTG GGATGGAGGA GACTCGAATG
    AGGGCTTCCA CACTGCTGTC TAAGGTCTTC CTGCAGCACC TGTCTCCACT GCTGTCACTC
    TCTACCTTTG CGGCCCTCTG GCTGACCATC CTGGACTTCA TGGACAAGTA TATGCATGCA
    GGATCCAGTG ACTTACTGTC AGAGGCCATC CCTGAGTCTC TGAAGAACAT GCTCCTGGTG
    ATGGACACAG CAGAGATTTT CCACAATGCA GATGCCCGAG GAGGCAGCCC CTCAGCCCTC
    TGGGAGATCA CCTGGGAGCG CATTGACTGT TTTCTGCCCC ACCTACGAGA TGAGCTCTTC
    AAGCAGACTG TCATCCAGGA CCCCATGCCA CTGGAGCCAC ATGCCCAAAA AACTCTGGCC
    TCAGCCCACA TGACTCCTGC TGCTGGAGAC ACAAGGACAC CTGGCCATCC ACCTCCCCCA
    GAGATGCCTT CTGAGCTGGG GGCCTGTGAC TTCGAGAAGC CTGAGGGCCC TCGACCTGCC
    AACAGCAGCT CTCCTGGATC ACCAGTGGCA TCTAGCCCCA ACAGACTGAG CCCTACTTCA
    GATGGGCCTC CTCCTCTGGC TCAGCCTCCA CTGATCCTGC AGCCCTTGGC CTCCCCATTG
    CAGGTGGGCG TACCACCCAT GACTCTACCA ATCATCCTCA ACCCTGCTCT CATTGAGGCC
    ACTTCACCAG TGCCCCTCCT GGCCACACCC CGCCCCACAG ACCCAATGCC CACCTCCGAG
    GTCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27951
    Clone ID Related Accession (Same CDS sequence) XM_005637661.3 , XM_005637661.2
    Accession Version XM_005637661.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5589bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726109.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005637661.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
    AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA
    CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT
    CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC
    ATGAGTGATT CATCCAAGTG GAAGAAACAG AAGAGATCCC CCCGGCCCCT GCGCCATATG
    ACCAAAGTAA CATCAGGTTC AGAGTTGCCC ACCTCCAATG GAACCACCTT ATCCTCTAAC
    CTCACTGGGG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAA CGGTGGTCAG CCCCTCTACA GACAGTGGCC TGGAGTTCTC CTCTCAGACC
    ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAACCTGGCT CTCCAGGGTA TAGCACAGCT
    ACAGAGCCTG GTAGCAGTGA GCTAGGGGTT CCTGAGCAGC CTGACCCGCA GCAGGAAGGA
    GTCCATGTGG AAAAGGCCCA GTCAGCATCT GTGGAATCCA TCCCCGAAGT GTTAGAGGAG
    TGCACGTCTC CTGCTGACCA CTCTGACTCT GCCTCTGTCC ATGACATGGA TTACGTCAAT
    CCCCGGGGTG TGCGCTTTAC ACAGTCCTCC CAGAAAGAAG GTACAGCTTT GGTTCCCTAT
    GGTCTTCCCT GCATCCGTGA GCTCTTCCGC TTCCTCATCT CCCTCACCAA TCCACATGAC
    CGCCACAATT CAGAAGTTAT GATCCACATG GGACTGCATT TGCTGACAGT GGCTCTTGAA
    TCTGCCCCTG TAGCCCAGTG CCAAACCCTT TTGGGCCTCA TCAAAGATGA GATGTGCCGC
    CACTTACTCC AGCTACTCAG TGTAGAGCGA CTCAACCTTT ATGCTGCTTC CCTGCGGGTA
    TGCTTCCTGC TTTTTGAGAG CATGAGGGAA CATCTCAAGT TCCAATTGGA GATGTACATC
    AAAAAGCTCA TGGAGATCAT CACTGTGGAG AACCCCAAAA TGCCTTATGA GATGAAAGAG
    ATGGCACTGG AGGCCATTGT GCAGCTCTGG CACATCCCCA GTTTTGTCAC TGAGCTCTAT
    ATCAACTATG ATTGTGACTA CTACTGTTCA AACCTCTTTG AAGACCTCAC CAAGCTGCTG
    TCCAAGAATG CCTTCCCTGT GTCTGGTCAG CTCTATACAA CACACCTCCT GTCTCTTGAT
    GCCCTGTTGA CAGTGATTGA CAGCACTGAG GCCCATTGCC AGGCCAAAGT CCTCAACAGT
    CTTACCCAGC AAGAGAAGAA AGAAGCAGCC AGATCTGGCT ATGAGGCGGT GGATGGTACC
    CGAGAATCCA GCAATAGTGA GAGAGCAGCC AGCGATGGGA AACCTGTAGG CATAGCCGCA
    GACATCCCAG GTCTACATCT GCCAGGTGGA GGGCGGCTGC CAGCAGAACA TGGGAAGCCA
    GGATGCAGTG ATCTGGAGGA AGCTGCTGAC TCTGGGGCTG ACAAAAAGTT TACCCGGAAG
    CCACCTCGAT TTTCCTGTCT CCTGCCAGAT CCACGGGAAC TGATTGAAAT TAAAAACAAA
    AAGAAGCTGC TAATCACTGG CACAGAGCAG TTCAACCAGA AACCAAAGAA GGGAATCCAG
    TTTCTGCAGG AGAAAGGCCT CCTCACCATC CCAATGGACA ACACAGAGGT AGCCCAGTGG
    CTCCGAGAGA ACCCTCGGCT GGACAAGAAA ATGATTGGAG AGTTTGTGAG TGACCGCAAA
    AACATTGACC TGTTGGAGAG CTTTGTGAGC ACCTTCAGCT TTCAGGGTCT GCGGCTGGAT
    GAAGCTCTTC GTCTCTACCT GGAAGCCTTT CGCTTACCTG GGGAAGCACC AGTCATCCAG
    AGGTTGCTGG AGGCATTCAC AGAGCATTGG AGGAATTGTA ATGGCTCCCC ATTTGCCAAT
    AGCGATGCCT GCTTTGCTCT GGCCTATGCT GTCATCTTGC TTAATACTGA CCAGCACAAC
    CACAACGTTC GCAAACAGAA TGCACCCATG ACTCTAGAGG AGTTTCGCAA AAACCTAAAA
    GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AGGACATGTA CCATGCCATC
    AAGAATGAGG AAATTGTGAT GCCTGAAGAG CAGACAGGCT TGGTTCGGGA GAACTATGTG
    TGGAATGTGC TACTTCATCG AGGTGCCACC CCAGAGGGCA TATTCCTTCG TGTGCCTGCT
    GGCAGCTATG ATCTTGACCT CTTCACCATG ACGTGGGGCC CCACTATTGC TGCCCTTTCT
    TATGTCTTTG ACAAAAGCCT TGAGGAAACA ATTATCCAGA AAGCCATCTC AGGCTTCAGG
    AAGTGCGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATA
    TCTCTGTGCA AATTCACAGC TCTTAGCAGT GAGTCTATTG AGAATCTGCC CAGTGTGTTT
    GGAAGCAACC CCAAAGCCCA CATTGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT
    GGTGATATCC TGCGGGAGGG CTGGAAGAAT ATCATGGAAG CCATGCTGCA GCTCTTCCGA
    GCCCAACTGC TTCCCAAAGC TATGGTGGAG GTAGAAGATT TTGTGGATCC CAATGGCAAG
    ATCTCTCTAC AGAGGGAGGA GACACCATCA AACCGAGGAG AGTCGACAGT GCTAAGCTTT
    GTGAGCTGGC TAACACTAAG TGGTACTGAG CAGTCTAGTG TGCGGGGCCC ATCCACTGAG
    AACCAAGAGG CCAAAAGAGC GGCCTTGGAC TGCATCAAGC ATTGTGACCC AGAAAAGATG
    ATCACAGAAA GCAAGTTCCT TCAGCTGGAG TCACTGCAGG AGCTCATGAA GGCTCTGGTC
    TCAGTGACGC CAGATGAAGA GACATATGAT GAGGAGGATG CTGCTTTCTG CCTAGAGATG
    CTGCTGAGGA TTGTGCTGGA GAACAGGGAC CGTGTGGGTT GTGTGTGGCA GACTGTCCGA
    GACCATCTAT ATCACCTGTG TGTCCAGGCA CAGGATTTCT GCTTCCTTGT GGAGCGGGCA
    GTGGTGGGGC TGCTCCGCTT GGCCATTCGA CTACTCCGAA GAGAAGAGAT CAGTGGCCAG
    GTGCTGCTCT CCCTGCGTAT TTTGCTACTA ATGAAGCCCA GCGTGCTGTC CCGAGTCAGT
    CACCAGGTAG CCTATGGGCT CCATGAACTT CTTAAGACCA ACGCAGCCAA CATCCACTCA
    GGTGACGACT GGGCCACTCT CTTCACACTG CTGGAATGCA TTGGCTCAGG TGTAAAGCCT
    CCAGCTGCCC TGCAGGCCAC AGCCAGGGCT GAAGCACCTG ATGCCGGGGC CCAATCAGAC
    AGCGAACTCC CATCCTACCA TCAGAATGAT GTGAGCCTGG ACCGAGGATA CACTTCTGAC
    TCGGAGGTCT ATGCTGACCA TTGCAGGCCT GGCAAGATCC ACCGATCAGC CACTGATGCT
    GATGTAGTCA ACAGCGGCTG GTTAGTGGTG GGGAAGGATG ACATTGATAA CTCCAAGCCA
    GGTCCTGGGC CCAGCCGGCT AGGCCCTTCA CCCCTGGTCA ATCAGTACAG CCTAACAGTG
    GGGCTGGACC TCGGGCCACA CGACACTAAG TCCCTGCTTA AATGTGTAGA ATCTCTGTCC
    TTCATTGTGC GTGATGCTGC CCACATTACA CCTGACAACT TTGAGCTCTG CGTGAAGACT
    CTCCGTATCT TTGTGGAGGC CAGTCTGAAT GGCGGGTGCA AGTCCCAGGA GAAACGTGGC
    AAGAGTCACA AATATGACAG CAAAGGAAAC CGCTTCAAGA AGAAATCCAA GGAAGGTTCA
    GTGCTTCGGC GGCCTCGAAC CTCTGGCCAA CATGCCACTC GGGGTGGGCA TAGTGACGAT
    GATGAGGATG AAGGGGTGCC TGCCAGCTAC CATACGGTGT CTTTACAGGT CAGTCAGGAC
    TTGTTAGATC TGATGCACAC CCTGCACACG CGAGCAGCCT CTATCTACAG CTCTTGGGCG
    GAGGAACAGC GCCACCTAGA GACAGGTGGC CGGAAGATAG AAGCGGATTC ACGTACCCTC
    TGGGCCCACT GCTGGTGTCC CTTACTGCAG GGCATTGCCT GCTTGTGCTG TGATGCCCGG
    CGCCAGGTGC GGATGCAAGC ACTGACTTAT CTGCAACGAG CACTACTGGT ACATGATCTG
    CAGAAGCTAG ATGCCCTGGA GTGGGAGTCC TGTTTTAACA AGGTGCTGTT TCCTCTACTG
    ACCAAACTCT TGGAGAACAT CAGCCCTGCA GATGTGGGTG GGATGGAGGA GACTCGAATG
    AGGGCTTCCA CACTGCTGTC TAAGGTCTTC CTGCAGCACC TGTCTCCACT GCTGTCACTC
    TCTACCTTTG CGGCCCTCTG GCTGACCATC CTGGACTTCA TGGACAAGTA TATGCATGCA
    GGATCCAGTG ACTTACTGTC AGAGGCCATC CCTGAGTCTC TGAAGAACAT GCTCCTGGTG
    ATGGACACAG CAGAGATTTT CCACAATGCA GATGCCCGAG GAGGCAGCCC CTCAGCCCTC
    TGGGAGATCA CCTGGGAGCG CATTGACTGT TTTCTGCCCC ACCTACGAGA TGAGCTCTTC
    AAGCAGACTG TCATCCAGGA CCCCATGCCA CTGGAGCCAC ATGCCCAAAA AACTCTGGCC
    TCAGCCCACA TGACTCCTGC TGCTGGAGAC ACAAGGACAC CTGGCCATCC ACCTCCCCCA
    GAGATGCCTT CTGAGCTGGG GGCCTGTGAC TTCGAGAAGC CTGAGGGCCC TCGACCTGCC
    AACAGCAGCT CTCCTGGATC ACCAGTGGCA TCTAGCCCCA ACAGACTGAG CCCTACTTCA
    GATGGGCCTC CTCCTCTGGC TCAGCCTCCA CTGATCCTGC AGCCCTTGGC CTCCCCATTG
    CAGGTGGGCG TACCACCCAT GACTCTACCA ATCATCCTCA ACCCTGCTCT CATTGAGGCC
    ACTTCACCAG TGCCCCTCCT GGCCACACCC CGCCCCACAG ACCCAATGCC CACCTCCGAG
    GTCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005637718.1
    CDS251..5839
    Translation

    Target ORF information:

    RefSeq Version XM_005637661.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005637661.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
    AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA
    CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT
    CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC
    ATGAGTGATT CATCCAAGTG GAAGAAACAG AAGAGATCCC CCCGGCCCCT GCGCCATATG
    ACCAAAGTAA CATCAGGTTC AGAGTTGCCC ACCTCCAATG GAACCACCTT ATCCTCTAAC
    CTCACTGGGG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAA CGGTGGTCAG CCCCTCTACA GACAGTGGCC TGGAGTTCTC CTCTCAGACC
    ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAACCTGGCT CTCCAGGGTA TAGCACAGCT
    ACAGAGCCTG GTAGCAGTGA GCTAGGGGTT CCTGAGCAGC CTGACCCGCA GCAGGAAGGA
    GTCCATGTGG AAAAGGCCCA GTCAGCATCT GTGGAATCCA TCCCCGAAGT GTTAGAGGAG
    TGCACGTCTC CTGCTGACCA CTCTGACTCT GCCTCTGTCC ATGACATGGA TTACGTCAAT
    CCCCGGGGTG TGCGCTTTAC ACAGTCCTCC CAGAAAGAAG GTACAGCTTT GGTTCCCTAT
    GGTCTTCCCT GCATCCGTGA GCTCTTCCGC TTCCTCATCT CCCTCACCAA TCCACATGAC
    CGCCACAATT CAGAAGTTAT GATCCACATG GGACTGCATT TGCTGACAGT GGCTCTTGAA
    TCTGCCCCTG TAGCCCAGTG CCAAACCCTT TTGGGCCTCA TCAAAGATGA GATGTGCCGC
    CACTTACTCC AGCTACTCAG TGTAGAGCGA CTCAACCTTT ATGCTGCTTC CCTGCGGGTA
    TGCTTCCTGC TTTTTGAGAG CATGAGGGAA CATCTCAAGT TCCAATTGGA GATGTACATC
    AAAAAGCTCA TGGAGATCAT CACTGTGGAG AACCCCAAAA TGCCTTATGA GATGAAAGAG
    ATGGCACTGG AGGCCATTGT GCAGCTCTGG CACATCCCCA GTTTTGTCAC TGAGCTCTAT
    ATCAACTATG ATTGTGACTA CTACTGTTCA AACCTCTTTG AAGACCTCAC CAAGCTGCTG
    TCCAAGAATG CCTTCCCTGT GTCTGGTCAG CTCTATACAA CACACCTCCT GTCTCTTGAT
    GCCCTGTTGA CAGTGATTGA CAGCACTGAG GCCCATTGCC AGGCCAAAGT CCTCAACAGT
    CTTACCCAGC AAGAGAAGAA AGAAGCAGCC AGATCTGGCT ATGAGGCGGT GGATGGTACC
    CGAGAATCCA GCAATAGTGA GAGAGCAGCC AGCGATGGGA AACCTGTAGG CATAGCCGCA
    GACATCCCAG GTCTACATCT GCCAGGTGGA GGGCGGCTGC CAGCAGAACA TGGGAAGCCA
    GGATGCAGTG ATCTGGAGGA AGCTGCTGAC TCTGGGGCTG ACAAAAAGTT TACCCGGAAG
    CCACCTCGAT TTTCCTGTCT CCTGCCAGAT CCACGGGAAC TGATTGAAAT TAAAAACAAA
    AAGAAGCTGC TAATCACTGG CACAGAGCAG TTCAACCAGA AACCAAAGAA GGGAATCCAG
    TTTCTGCAGG AGAAAGGCCT CCTCACCATC CCAATGGACA ACACAGAGGT AGCCCAGTGG
    CTCCGAGAGA ACCCTCGGCT GGACAAGAAA ATGATTGGAG AGTTTGTGAG TGACCGCAAA
    AACATTGACC TGTTGGAGAG CTTTGTGAGC ACCTTCAGCT TTCAGGGTCT GCGGCTGGAT
    GAAGCTCTTC GTCTCTACCT GGAAGCCTTT CGCTTACCTG GGGAAGCACC AGTCATCCAG
    AGGTTGCTGG AGGCATTCAC AGAGCATTGG AGGAATTGTA ATGGCTCCCC ATTTGCCAAT
    AGCGATGCCT GCTTTGCTCT GGCCTATGCT GTCATCTTGC TTAATACTGA CCAGCACAAC
    CACAACGTTC GCAAACAGAA TGCACCCATG ACTCTAGAGG AGTTTCGCAA AAACCTAAAA
    GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AGGACATGTA CCATGCCATC
    AAGAATGAGG AAATTGTGAT GCCTGAAGAG CAGACAGGCT TGGTTCGGGA GAACTATGTG
    TGGAATGTGC TACTTCATCG AGGTGCCACC CCAGAGGGCA TATTCCTTCG TGTGCCTGCT
    GGCAGCTATG ATCTTGACCT CTTCACCATG ACGTGGGGCC CCACTATTGC TGCCCTTTCT
    TATGTCTTTG ACAAAAGCCT TGAGGAAACA ATTATCCAGA AAGCCATCTC AGGCTTCAGG
    AAGTGCGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATA
    TCTCTGTGCA AATTCACAGC TCTTAGCAGT GAGTCTATTG AGAATCTGCC CAGTGTGTTT
    GGAAGCAACC CCAAAGCCCA CATTGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT
    GGTGATATCC TGCGGGAGGG CTGGAAGAAT ATCATGGAAG CCATGCTGCA GCTCTTCCGA
    GCCCAACTGC TTCCCAAAGC TATGGTGGAG GTAGAAGATT TTGTGGATCC CAATGGCAAG
    ATCTCTCTAC AGAGGGAGGA GACACCATCA AACCGAGGAG AGTCGACAGT GCTAAGCTTT
    GTGAGCTGGC TAACACTAAG TGGTACTGAG CAGTCTAGTG TGCGGGGCCC ATCCACTGAG
    AACCAAGAGG CCAAAAGAGC GGCCTTGGAC TGCATCAAGC ATTGTGACCC AGAAAAGATG
    ATCACAGAAA GCAAGTTCCT TCAGCTGGAG TCACTGCAGG AGCTCATGAA GGCTCTGGTC
    TCAGTGACGC CAGATGAAGA GACATATGAT GAGGAGGATG CTGCTTTCTG CCTAGAGATG
    CTGCTGAGGA TTGTGCTGGA GAACAGGGAC CGTGTGGGTT GTGTGTGGCA GACTGTCCGA
    GACCATCTAT ATCACCTGTG TGTCCAGGCA CAGGATTTCT GCTTCCTTGT GGAGCGGGCA
    GTGGTGGGGC TGCTCCGCTT GGCCATTCGA CTACTCCGAA GAGAAGAGAT CAGTGGCCAG
    GTGCTGCTCT CCCTGCGTAT TTTGCTACTA ATGAAGCCCA GCGTGCTGTC CCGAGTCAGT
    CACCAGGTAG CCTATGGGCT CCATGAACTT CTTAAGACCA ACGCAGCCAA CATCCACTCA
    GGTGACGACT GGGCCACTCT CTTCACACTG CTGGAATGCA TTGGCTCAGG TGTAAAGCCT
    CCAGCTGCCC TGCAGGCCAC AGCCAGGGCT GAAGCACCTG ATGCCGGGGC CCAATCAGAC
    AGCGAACTCC CATCCTACCA TCAGAATGAT GTGAGCCTGG ACCGAGGATA CACTTCTGAC
    TCGGAGGTCT ATGCTGACCA TTGCAGGCCT GGCAAGATCC ACCGATCAGC CACTGATGCT
    GATGTAGTCA ACAGCGGCTG GTTAGTGGTG GGGAAGGATG ACATTGATAA CTCCAAGCCA
    GGTCCTGGGC CCAGCCGGCT AGGCCCTTCA CCCCTGGTCA ATCAGTACAG CCTAACAGTG
    GGGCTGGACC TCGGGCCACA CGACACTAAG TCCCTGCTTA AATGTGTAGA ATCTCTGTCC
    TTCATTGTGC GTGATGCTGC CCACATTACA CCTGACAACT TTGAGCTCTG CGTGAAGACT
    CTCCGTATCT TTGTGGAGGC CAGTCTGAAT GGCGGGTGCA AGTCCCAGGA GAAACGTGGC
    AAGAGTCACA AATATGACAG CAAAGGAAAC CGCTTCAAGA AGAAATCCAA GGAAGGTTCA
    GTGCTTCGGC GGCCTCGAAC CTCTGGCCAA CATGCCACTC GGGGTGGGCA TAGTGACGAT
    GATGAGGATG AAGGGGTGCC TGCCAGCTAC CATACGGTGT CTTTACAGGT CAGTCAGGAC
    TTGTTAGATC TGATGCACAC CCTGCACACG CGAGCAGCCT CTATCTACAG CTCTTGGGCG
    GAGGAACAGC GCCACCTAGA GACAGGTGGC CGGAAGATAG AAGCGGATTC ACGTACCCTC
    TGGGCCCACT GCTGGTGTCC CTTACTGCAG GGCATTGCCT GCTTGTGCTG TGATGCCCGG
    CGCCAGGTGC GGATGCAAGC ACTGACTTAT CTGCAACGAG CACTACTGGT ACATGATCTG
    CAGAAGCTAG ATGCCCTGGA GTGGGAGTCC TGTTTTAACA AGGTGCTGTT TCCTCTACTG
    ACCAAACTCT TGGAGAACAT CAGCCCTGCA GATGTGGGTG GGATGGAGGA GACTCGAATG
    AGGGCTTCCA CACTGCTGTC TAAGGTCTTC CTGCAGCACC TGTCTCCACT GCTGTCACTC
    TCTACCTTTG CGGCCCTCTG GCTGACCATC CTGGACTTCA TGGACAAGTA TATGCATGCA
    GGATCCAGTG ACTTACTGTC AGAGGCCATC CCTGAGTCTC TGAAGAACAT GCTCCTGGTG
    ATGGACACAG CAGAGATTTT CCACAATGCA GATGCCCGAG GAGGCAGCCC CTCAGCCCTC
    TGGGAGATCA CCTGGGAGCG CATTGACTGT TTTCTGCCCC ACCTACGAGA TGAGCTCTTC
    AAGCAGACTG TCATCCAGGA CCCCATGCCA CTGGAGCCAC ATGCCCAAAA AACTCTGGCC
    TCAGCCCACA TGACTCCTGC TGCTGGAGAC ACAAGGACAC CTGGCCATCC ACCTCCCCCA
    GAGATGCCTT CTGAGCTGGG GGCCTGTGAC TTCGAGAAGC CTGAGGGCCC TCGACCTGCC
    AACAGCAGCT CTCCTGGATC ACCAGTGGCA TCTAGCCCCA ACAGACTGAG CCCTACTTCA
    GATGGGCCTC CTCCTCTGGC TCAGCCTCCA CTGATCCTGC AGCCCTTGGC CTCCCCATTG
    CAGGTGGGCG TACCACCCAT GACTCTACCA ATCATCCTCA ACCCTGCTCT CATTGAGGCC
    ACTTCACCAG TGCCCCTCCT GGCCACACCC CGCCCCACAG ACCCAATGCC CACCTCCGAG
    GTCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27952
    Clone ID Related Accession (Same CDS sequence) XM_543987.5 , XM_543987.6
    Accession Version XM_543987.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5577bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726109.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_543987.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
    AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA
    CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT
    CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC
    ATGAGTGATT CATCCAAGTG GAAGAAACAG AAGAGATCCC CCCGGCCCCT GCGCCATATG
    ACCAAAGTAA CATCAGGTTC AGAGTTGCCC ACCTCCAATG GAACCACCTT ATCCTCTAAC
    CTCACTGGGG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAA CGGTGGTCAG CCCCTCTACA GACAGTGGCC TGGAGTTCTC CTCTCAGACC
    ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAACCTGGCT CTCCAGGGTA TAGCACAGCT
    ACAGAGCCTG GTAGCAGTGA GCTAGGGGTT CCTGAGCAGC CTGACCCGCA GCAGGAAGGA
    GTCCATGTGG AAAAGGCCCA GTCAGCATCT GTGGAATCCA TCCCCGAAGT GTTAGAGGAG
    TGCACGTCTC CTGCTGACCA CTCTGACTCT GCCTCTGTCC ATGACATGGA TTACGTCAAT
    CCCCGGGGTG TGCGCTTTAC ACAGTCCTCC CAGAAAGAAG GTACAGCTTT GGTTCCCTAT
    GGTCTTCCCT GCATCCGTGA GCTCTTCCGC TTCCTCATCT CCCTCACCAA TCCACATGAC
    CGCCACAATT CAGAAGTTAT GATCCACATG GGACTGCATT TGCTGACAGT GGCTCTTGAA
    TCTGCCCCTG TAGCCCAGTG CCAAACCCTT TTGGGCCTCA TCAAAGATGA GATGTGCCGC
    CACTTACTCC AGCTACTCAG TGTAGAGCGA CTCAACCTTT ATGCTGCTTC CCTGCGGGTA
    TGCTTCCTGC TTTTTGAGAG CATGAGGGAA CATCTCAAGT TCCAATTGGA GATGTACATC
    AAAAAGCTCA TGGAGATCAT CACTGTGGAG AACCCCAAAA TGCCTTATGA GATGAAAGAG
    ATGGCACTGG AGGCCATTGT GCAGCTCTGG CACATCCCCA GTTTTGTCAC TGAGCTCTAT
    ATCAACTATG ATTGTGACTA CTACTGTTCA AACCTCTTTG AAGACCTCAC CAAGCTGCTG
    TCCAAGAATG CCTTCCCTGT GTCTGGTCAG CTCTATACAA CACACCTCCT GTCTCTTGAT
    GCCCTGTTGA CAGTGATTGA CAGCACTGAG GCCCATTGCC AGGCCAAAGT CCTCAACAGT
    CTTACCCAGC AAGAGAAGAA AGAAGCAGCC AGATCTGGCT ATGAGGCGGT GGATGGTACC
    CGAGAATCCA GCAATAGTGA GAGAGCAGCC AGCGATGGGA AACCTGTAGG CATAGCCGCA
    GACATCCCAG GTCTACATCT GCCAGGTGGA GGGCGGCTGC CAGCAGAACA TGGGAAGCCA
    GGATGCAGTG ATCTGGAGGA AGCTGCTGAC TCTGGGGCTG ACAAAAAGTT TACCCGGAAG
    CCACCTCGAT TTTCCTGTCT CCTGCCAGAT CCACGGGAAC TGATTGAAAT TAAAAACAAA
    AAGAAGCTGC TAATCACTGG CACAGAGCAG TTCAACCAGA AACCAAAGAA GGGAATCCAG
    TTTCTGCAGG AGAAAGGCCT CCTCACCATC CCAATGGACA ACACAGAGGT AGCCCAGTGG
    CTCCGAGAGA ACCCTCGGCT GGACAAGAAA ATGATTGGAG AGTTTGTGAG TGACCGCAAA
    AACATTGACC TGTTGGAGAG CTTTGTGAGC ACCTTCAGCT TTCAGGGTCT GCGGCTGGAT
    GAAGCTCTTC GTCTCTACCT GGAAGCCTTT CGCTTACCTG GGGAAGCACC AGTCATCCAG
    AGGTTGCTGG AGGCATTCAC AGAGCATTGG AGGAATTGTA ATGGCTCCCC ATTTGCCAAT
    AGCGATGCCT GCTTTGCTCT GGCCTATGCT GTCATCTTGC TTAATACTGA CCAGCACAAC
    CACAACGTTC GCAAACAGAA TGCACCCATG ACTCTAGAGG AGTTTCGCAA AAACCTAAAA
    GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AGGACATGTA CCATGCCATC
    AAGAATGAGG AAATTGTGAT GCCTGAAGAG CAGACAGGCT TGGTTCGGGA GAACTATGTG
    TGGAATGTGC TACTTCATCG AGGTGCCACC CCAGAGGGCA TATTCCTTCG TGTGCCTGCT
    GGCAGCTATG ATCTTGACCT CTTCACCATG ACGTGGGGCC CCACTATTGC TGCCCTTTCT
    TATGTCTTTG ACAAAAGCCT TGAGGAAACA ATTATCCAGA AAGCCATCTC AGGCTTCAGG
    AAGTGCGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATA
    TCTCTGTGCA AATTCACAGC TCTTAGCAGT GAGTCTATTG AGAATCTGCC CAGTGTGTTT
    GGAAGCAACC CCAAAGCCCA CATTGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT
    GGTGATATCC TGCGGGAGGG CTGGAAGAAT ATCATGGAAG CCATGCTGCA GCTCTTCCGA
    GCCCAACTGC TTCCCAAAGC TATGGTGGAG GTAGAAGATT TTGTGGATCC CAATGGCAAG
    ATCTCTCTAC AGAGGGAGGA GACACCATCA AACCGAGGAG AGTCGACAGT GCTAAGCTTT
    GTGAGCTGGC TAACACTAAG TGGTACTGAG CAGTCTAGTG TGCGGGGCCC ATCCACTGAG
    AACCAAGAGG CCAAAAGAGC GGCCTTGGAC TGCATCAAGC ATTGTGACCC AGAAAAGATG
    ATCACAGAAA GCAAGTTCCT TCAGCTGGAG TCACTGCAGG AGCTCATGAA GGCTCTGGTC
    TCAGTGACGC CAGATGAAGA GACATATGAT GAGGAGGATG CTGCTTTCTG CCTAGAGATG
    CTGCTGAGGA TTGTGCTGGA GAACAGGGAC CGTGTGGGTT GTGTGTGGCA GACTGTCCGA
    GACCATCTAT ATCACCTGTG TGTCCAGGCA CAGGATTTCT GCTTCCTTGT GGAGCGGGCA
    GTGGTGGGGC TGCTCCGCTT GGCCATTCGA CTACTCCGAA GAGAAGAGAT CAGTGGCCAG
    GTGCTGCTCT CCCTGCGTAT TTTGCTACTA ATGAAGCCCA GCGTGCTGTC CCGAGTCAGT
    CACCAGGTAG CCTATGGGCT CCATGAACTT CTTAAGACCA ACGCAGCCAA CATCCACTCA
    GGTGACGACT GGGCCACTCT CTTCACACTG CTGGAATGCA TTGGCTCAGG TGTAAAGCCT
    CCAGCTGCCC TGCAGGCCAC AGCCAGGGCT GAAGCACCTG ATGCCGGGGC CCAATCAGAC
    AGCGAACTCC CATCCTACCA TCAGAATGAT GTGAGCCTGG ACCGAGGATA CACTTCTGAC
    TCGGAGGTCT ATGCTGACCA TTGCAGGCCT GGCAAGATCC ACCGATCAGC CACTGATGCT
    GATGTAGTCA ACAGCGGCTG GTTAGTGGTG GGGAAGGATG ACATTGATAA CTCCAAGCCA
    GGTCCTGGGC CCAGCCGGCT AGGCCCTTCA CCCCTGGTCA ATCAGTACAG CCTAACAGTG
    GGGCTGGACC TCGGGCCACA CGACACTAAG TCCCTGCTTA AATGTGTAGA ATCTCTGTCC
    TTCATTGTGC GTGATGCTGC CCACATTACA CCTGACAACT TTGAGCTCTG CGTGAAGACT
    CTCCGTATCT TTGTGGAGGC CAGTCTGAAT GGCGGGTGCA AGTCCCAGGA GAAACGTGGC
    AAGAGTCACA AATATGACAG CAAAGGAAAC CGCTTCAAGA AGAAATCCAA GGAAGGTTCA
    GTGCTTCGGC GGCCTCGAAC CTCTGGCCAA CATGCCACTC GGGGTGGGCA TAGTGACGAT
    GATGAGGATG AAGGGGTGCC TGCCAGCTAC CATACGGTGT CTTTACAGTT GTTAGATCTG
    ATGCACACCC TGCACACGCG AGCAGCCTCT ATCTACAGCT CTTGGGCGGA GGAACAGCGC
    CACCTAGAGA CAGGTGGCCG GAAGATAGAA GCGGATTCAC GTACCCTCTG GGCCCACTGC
    TGGTGTCCCT TACTGCAGGG CATTGCCTGC TTGTGCTGTG ATGCCCGGCG CCAGGTGCGG
    ATGCAAGCAC TGACTTATCT GCAACGAGCA CTACTGGTAC ATGATCTGCA GAAGCTAGAT
    GCCCTGGAGT GGGAGTCCTG TTTTAACAAG GTGCTGTTTC CTCTACTGAC CAAACTCTTG
    GAGAACATCA GCCCTGCAGA TGTGGGTGGG ATGGAGGAGA CTCGAATGAG GGCTTCCACA
    CTGCTGTCTA AGGTCTTCCT GCAGCACCTG TCTCCACTGC TGTCACTCTC TACCTTTGCG
    GCCCTCTGGC TGACCATCCT GGACTTCATG GACAAGTATA TGCATGCAGG ATCCAGTGAC
    TTACTGTCAG AGGCCATCCC TGAGTCTCTG AAGAACATGC TCCTGGTGAT GGACACAGCA
    GAGATTTTCC ACAATGCAGA TGCCCGAGGA GGCAGCCCCT CAGCCCTCTG GGAGATCACC
    TGGGAGCGCA TTGACTGTTT TCTGCCCCAC CTACGAGATG AGCTCTTCAA GCAGACTGTC
    ATCCAGGACC CCATGCCACT GGAGCCACAT GCCCAAAAAA CTCTGGCCTC AGCCCACATG
    ACTCCTGCTG CTGGAGACAC AAGGACACCT GGCCATCCAC CTCCCCCAGA GATGCCTTCT
    GAGCTGGGGG CCTGTGACTT CGAGAAGCCT GAGGGCCCTC GACCTGCCAA CAGCAGCTCT
    CCTGGATCAC CAGTGGCATC TAGCCCCAAC AGACTGAGCC CTACTTCAGA TGGGCCTCCT
    CCTCTGGCTC AGCCTCCACT GATCCTGCAG CCCTTGGCCT CCCCATTGCA GGTGGGCGTA
    CCACCCATGA CTCTACCAAT CATCCTCAAC CCTGCTCTCA TTGAGGCCAC TTCACCAGTG
    CCCCTCCTGG CCACACCCCG CCCCACAGAC CCAATGCCCA CCTCCGAGGT CAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_543987.2
    CDS251..5827
    Translation

    Target ORF information:

    RefSeq Version XM_543987.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_543987.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
    AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA
    CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT
    CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC
    ATGAGTGATT CATCCAAGTG GAAGAAACAG AAGAGATCCC CCCGGCCCCT GCGCCATATG
    ACCAAAGTAA CATCAGGTTC AGAGTTGCCC ACCTCCAATG GAACCACCTT ATCCTCTAAC
    CTCACTGGGG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAA CGGTGGTCAG CCCCTCTACA GACAGTGGCC TGGAGTTCTC CTCTCAGACC
    ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAACCTGGCT CTCCAGGGTA TAGCACAGCT
    ACAGAGCCTG GTAGCAGTGA GCTAGGGGTT CCTGAGCAGC CTGACCCGCA GCAGGAAGGA
    GTCCATGTGG AAAAGGCCCA GTCAGCATCT GTGGAATCCA TCCCCGAAGT GTTAGAGGAG
    TGCACGTCTC CTGCTGACCA CTCTGACTCT GCCTCTGTCC ATGACATGGA TTACGTCAAT
    CCCCGGGGTG TGCGCTTTAC ACAGTCCTCC CAGAAAGAAG GTACAGCTTT GGTTCCCTAT
    GGTCTTCCCT GCATCCGTGA GCTCTTCCGC TTCCTCATCT CCCTCACCAA TCCACATGAC
    CGCCACAATT CAGAAGTTAT GATCCACATG GGACTGCATT TGCTGACAGT GGCTCTTGAA
    TCTGCCCCTG TAGCCCAGTG CCAAACCCTT TTGGGCCTCA TCAAAGATGA GATGTGCCGC
    CACTTACTCC AGCTACTCAG TGTAGAGCGA CTCAACCTTT ATGCTGCTTC CCTGCGGGTA
    TGCTTCCTGC TTTTTGAGAG CATGAGGGAA CATCTCAAGT TCCAATTGGA GATGTACATC
    AAAAAGCTCA TGGAGATCAT CACTGTGGAG AACCCCAAAA TGCCTTATGA GATGAAAGAG
    ATGGCACTGG AGGCCATTGT GCAGCTCTGG CACATCCCCA GTTTTGTCAC TGAGCTCTAT
    ATCAACTATG ATTGTGACTA CTACTGTTCA AACCTCTTTG AAGACCTCAC CAAGCTGCTG
    TCCAAGAATG CCTTCCCTGT GTCTGGTCAG CTCTATACAA CACACCTCCT GTCTCTTGAT
    GCCCTGTTGA CAGTGATTGA CAGCACTGAG GCCCATTGCC AGGCCAAAGT CCTCAACAGT
    CTTACCCAGC AAGAGAAGAA AGAAGCAGCC AGATCTGGCT ATGAGGCGGT GGATGGTACC
    CGAGAATCCA GCAATAGTGA GAGAGCAGCC AGCGATGGGA AACCTGTAGG CATAGCCGCA
    GACATCCCAG GTCTACATCT GCCAGGTGGA GGGCGGCTGC CAGCAGAACA TGGGAAGCCA
    GGATGCAGTG ATCTGGAGGA AGCTGCTGAC TCTGGGGCTG ACAAAAAGTT TACCCGGAAG
    CCACCTCGAT TTTCCTGTCT CCTGCCAGAT CCACGGGAAC TGATTGAAAT TAAAAACAAA
    AAGAAGCTGC TAATCACTGG CACAGAGCAG TTCAACCAGA AACCAAAGAA GGGAATCCAG
    TTTCTGCAGG AGAAAGGCCT CCTCACCATC CCAATGGACA ACACAGAGGT AGCCCAGTGG
    CTCCGAGAGA ACCCTCGGCT GGACAAGAAA ATGATTGGAG AGTTTGTGAG TGACCGCAAA
    AACATTGACC TGTTGGAGAG CTTTGTGAGC ACCTTCAGCT TTCAGGGTCT GCGGCTGGAT
    GAAGCTCTTC GTCTCTACCT GGAAGCCTTT CGCTTACCTG GGGAAGCACC AGTCATCCAG
    AGGTTGCTGG AGGCATTCAC AGAGCATTGG AGGAATTGTA ATGGCTCCCC ATTTGCCAAT
    AGCGATGCCT GCTTTGCTCT GGCCTATGCT GTCATCTTGC TTAATACTGA CCAGCACAAC
    CACAACGTTC GCAAACAGAA TGCACCCATG ACTCTAGAGG AGTTTCGCAA AAACCTAAAA
    GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AGGACATGTA CCATGCCATC
    AAGAATGAGG AAATTGTGAT GCCTGAAGAG CAGACAGGCT TGGTTCGGGA GAACTATGTG
    TGGAATGTGC TACTTCATCG AGGTGCCACC CCAGAGGGCA TATTCCTTCG TGTGCCTGCT
    GGCAGCTATG ATCTTGACCT CTTCACCATG ACGTGGGGCC CCACTATTGC TGCCCTTTCT
    TATGTCTTTG ACAAAAGCCT TGAGGAAACA ATTATCCAGA AAGCCATCTC AGGCTTCAGG
    AAGTGCGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATA
    TCTCTGTGCA AATTCACAGC TCTTAGCAGT GAGTCTATTG AGAATCTGCC CAGTGTGTTT
    GGAAGCAACC CCAAAGCCCA CATTGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT
    GGTGATATCC TGCGGGAGGG CTGGAAGAAT ATCATGGAAG CCATGCTGCA GCTCTTCCGA
    GCCCAACTGC TTCCCAAAGC TATGGTGGAG GTAGAAGATT TTGTGGATCC CAATGGCAAG
    ATCTCTCTAC AGAGGGAGGA GACACCATCA AACCGAGGAG AGTCGACAGT GCTAAGCTTT
    GTGAGCTGGC TAACACTAAG TGGTACTGAG CAGTCTAGTG TGCGGGGCCC ATCCACTGAG
    AACCAAGAGG CCAAAAGAGC GGCCTTGGAC TGCATCAAGC ATTGTGACCC AGAAAAGATG
    ATCACAGAAA GCAAGTTCCT TCAGCTGGAG TCACTGCAGG AGCTCATGAA GGCTCTGGTC
    TCAGTGACGC CAGATGAAGA GACATATGAT GAGGAGGATG CTGCTTTCTG CCTAGAGATG
    CTGCTGAGGA TTGTGCTGGA GAACAGGGAC CGTGTGGGTT GTGTGTGGCA GACTGTCCGA
    GACCATCTAT ATCACCTGTG TGTCCAGGCA CAGGATTTCT GCTTCCTTGT GGAGCGGGCA
    GTGGTGGGGC TGCTCCGCTT GGCCATTCGA CTACTCCGAA GAGAAGAGAT CAGTGGCCAG
    GTGCTGCTCT CCCTGCGTAT TTTGCTACTA ATGAAGCCCA GCGTGCTGTC CCGAGTCAGT
    CACCAGGTAG CCTATGGGCT CCATGAACTT CTTAAGACCA ACGCAGCCAA CATCCACTCA
    GGTGACGACT GGGCCACTCT CTTCACACTG CTGGAATGCA TTGGCTCAGG TGTAAAGCCT
    CCAGCTGCCC TGCAGGCCAC AGCCAGGGCT GAAGCACCTG ATGCCGGGGC CCAATCAGAC
    AGCGAACTCC CATCCTACCA TCAGAATGAT GTGAGCCTGG ACCGAGGATA CACTTCTGAC
    TCGGAGGTCT ATGCTGACCA TTGCAGGCCT GGCAAGATCC ACCGATCAGC CACTGATGCT
    GATGTAGTCA ACAGCGGCTG GTTAGTGGTG GGGAAGGATG ACATTGATAA CTCCAAGCCA
    GGTCCTGGGC CCAGCCGGCT AGGCCCTTCA CCCCTGGTCA ATCAGTACAG CCTAACAGTG
    GGGCTGGACC TCGGGCCACA CGACACTAAG TCCCTGCTTA AATGTGTAGA ATCTCTGTCC
    TTCATTGTGC GTGATGCTGC CCACATTACA CCTGACAACT TTGAGCTCTG CGTGAAGACT
    CTCCGTATCT TTGTGGAGGC CAGTCTGAAT GGCGGGTGCA AGTCCCAGGA GAAACGTGGC
    AAGAGTCACA AATATGACAG CAAAGGAAAC CGCTTCAAGA AGAAATCCAA GGAAGGTTCA
    GTGCTTCGGC GGCCTCGAAC CTCTGGCCAA CATGCCACTC GGGGTGGGCA TAGTGACGAT
    GATGAGGATG AAGGGGTGCC TGCCAGCTAC CATACGGTGT CTTTACAGTT GTTAGATCTG
    ATGCACACCC TGCACACGCG AGCAGCCTCT ATCTACAGCT CTTGGGCGGA GGAACAGCGC
    CACCTAGAGA CAGGTGGCCG GAAGATAGAA GCGGATTCAC GTACCCTCTG GGCCCACTGC
    TGGTGTCCCT TACTGCAGGG CATTGCCTGC TTGTGCTGTG ATGCCCGGCG CCAGGTGCGG
    ATGCAAGCAC TGACTTATCT GCAACGAGCA CTACTGGTAC ATGATCTGCA GAAGCTAGAT
    GCCCTGGAGT GGGAGTCCTG TTTTAACAAG GTGCTGTTTC CTCTACTGAC CAAACTCTTG
    GAGAACATCA GCCCTGCAGA TGTGGGTGGG ATGGAGGAGA CTCGAATGAG GGCTTCCACA
    CTGCTGTCTA AGGTCTTCCT GCAGCACCTG TCTCCACTGC TGTCACTCTC TACCTTTGCG
    GCCCTCTGGC TGACCATCCT GGACTTCATG GACAAGTATA TGCATGCAGG ATCCAGTGAC
    TTACTGTCAG AGGCCATCCC TGAGTCTCTG AAGAACATGC TCCTGGTGAT GGACACAGCA
    GAGATTTTCC ACAATGCAGA TGCCCGAGGA GGCAGCCCCT CAGCCCTCTG GGAGATCACC
    TGGGAGCGCA TTGACTGTTT TCTGCCCCAC CTACGAGATG AGCTCTTCAA GCAGACTGTC
    ATCCAGGACC CCATGCCACT GGAGCCACAT GCCCAAAAAA CTCTGGCCTC AGCCCACATG
    ACTCCTGCTG CTGGAGACAC AAGGACACCT GGCCATCCAC CTCCCCCAGA GATGCCTTCT
    GAGCTGGGGG CCTGTGACTT CGAGAAGCCT GAGGGCCCTC GACCTGCCAA CAGCAGCTCT
    CCTGGATCAC CAGTGGCATC TAGCCCCAAC AGACTGAGCC CTACTTCAGA TGGGCCTCCT
    CCTCTGGCTC AGCCTCCACT GATCCTGCAG CCCTTGGCCT CCCCATTGCA GGTGGGCGTA
    CCACCCATGA CTCTACCAAT CATCCTCAAC CCTGCTCTCA TTGAGGCCAC TTCACCAGTG
    CCCCTCCTGG CCACACCCCG CCCCACAGAC CCAATGCCCA CCTCCGAGGT CAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27952
    Clone ID Related Accession (Same CDS sequence) XM_543987.5 , XM_543987.6
    Accession Version XM_543987.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5577bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006610.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_543987.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
    AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA
    CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT
    CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC
    ATGAGTGATT CATCCAAGTG GAAGAAACAG AAGAGATCCC CCCGGCCCCT GCGCCATATG
    ACCAAAGTAA CATCAGGTTC AGAGTTGCCC ACCTCCAATG GAACCACCTT ATCCTCTAAC
    CTCACTGGGG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAA CGGTGGTCAG CCCCTCTACA GACAGTGGCC TGGAGTTCTC CTCTCAGACC
    ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAACCTGGCT CTCCAGGGTA TAGCACAGCT
    ACAGAGCCTG GTAGCAGTGA GCTAGGGGTT CCTGAGCAGC CTGACCCGCA GCAGGAAGGA
    GTCCATGTGG AAAAGGCCCA GTCAGCATCT GTGGAATCCA TCCCCGAAGT GTTAGAGGAG
    TGCACGTCTC CTGCTGACCA CTCTGACTCT GCCTCTGTCC ATGACATGGA TTACGTCAAT
    CCCCGGGGTG TGCGCTTTAC ACAGTCCTCC CAGAAAGAAG GTACAGCTTT GGTTCCCTAT
    GGTCTTCCCT GCATCCGTGA GCTCTTCCGC TTCCTCATCT CCCTCACCAA TCCACATGAC
    CGCCACAATT CAGAAGTTAT GATCCACATG GGACTGCATT TGCTGACAGT GGCTCTTGAA
    TCTGCCCCTG TAGCCCAGTG CCAAACCCTT TTGGGCCTCA TCAAAGATGA GATGTGCCGC
    CACTTACTCC AGCTACTCAG TGTAGAGCGA CTCAACCTTT ATGCTGCTTC CCTGCGGGTA
    TGCTTCCTGC TTTTTGAGAG CATGAGGGAA CATCTCAAGT TCCAATTGGA GATGTACATC
    AAAAAGCTCA TGGAGATCAT CACTGTGGAG AACCCCAAAA TGCCTTATGA GATGAAAGAG
    ATGGCACTGG AGGCCATTGT GCAGCTCTGG CACATCCCCA GTTTTGTCAC TGAGCTCTAT
    ATCAACTATG ATTGTGACTA CTACTGTTCA AACCTCTTTG AAGACCTCAC CAAGCTGCTG
    TCCAAGAATG CCTTCCCTGT GTCTGGTCAG CTCTATACAA CACACCTCCT GTCTCTTGAT
    GCCCTGTTGA CAGTGATTGA CAGCACTGAG GCCCATTGCC AGGCCAAAGT CCTCAACAGT
    CTTACCCAGC AAGAGAAGAA AGAAGCAGCC AGATCTGGCT ATGAGGCGGT GGATGGTACC
    CGAGAATCCA GCAATAGTGA GAGAGCAGCC AGCGATGGGA AACCTGTAGG CATAGCCGCA
    GACATCCCAG GTCTACATCT GCCAGGTGGA GGGCGGCTGC CAGCAGAACA TGGGAAGCCA
    GGATGCAGTG ATCTGGAGGA AGCTGCTGAC TCTGGGGCTG ACAAAAAGTT TACCCGGAAG
    CCACCTCGAT TTTCCTGTCT CCTGCCAGAT CCACGGGAAC TGATTGAAAT TAAAAACAAA
    AAGAAGCTGC TAATCACTGG CACAGAGCAG TTCAACCAGA AACCAAAGAA GGGAATCCAG
    TTTCTGCAGG AGAAAGGCCT CCTCACCATC CCAATGGACA ACACAGAGGT AGCCCAGTGG
    CTCCGAGAGA ACCCTCGGCT GGACAAGAAA ATGATTGGAG AGTTTGTGAG TGACCGCAAA
    AACATTGACC TGTTGGAGAG CTTTGTGAGC ACCTTCAGCT TTCAGGGTCT GCGGCTGGAT
    GAAGCTCTTC GTCTCTACCT GGAAGCCTTT CGCTTACCTG GGGAAGCACC AGTCATCCAG
    AGGTTGCTGG AGGCATTCAC AGAGCATTGG AGGAATTGTA ATGGCTCCCC ATTTGCCAAT
    AGCGATGCCT GCTTTGCTCT GGCCTATGCT GTCATCTTGC TTAATACTGA CCAGCACAAC
    CACAACGTTC GCAAACAGAA TGCACCCATG ACTCTAGAGG AGTTTCGCAA AAACCTAAAA
    GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AGGACATGTA CCATGCCATC
    AAGAATGAGG AAATTGTGAT GCCTGAAGAG CAGACAGGCT TGGTTCGGGA GAACTATGTG
    TGGAATGTGC TACTTCATCG AGGTGCCACC CCAGAGGGCA TATTCCTTCG TGTGCCTGCT
    GGCAGCTATG ATCTTGACCT CTTCACCATG ACGTGGGGCC CCACTATTGC TGCCCTTTCT
    TATGTCTTTG ACAAAAGCCT TGAGGAAACA ATTATCCAGA AAGCCATCTC AGGCTTCAGG
    AAGTGCGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATA
    TCTCTGTGCA AATTCACAGC TCTTAGCAGT GAGTCTATTG AGAATCTGCC CAGTGTGTTT
    GGAAGCAACC CCAAAGCCCA CATTGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT
    GGTGATATCC TGCGGGAGGG CTGGAAGAAT ATCATGGAAG CCATGCTGCA GCTCTTCCGA
    GCCCAACTGC TTCCCAAAGC TATGGTGGAG GTAGAAGATT TTGTGGATCC CAATGGCAAG
    ATCTCTCTAC AGAGGGAGGA GACACCATCA AACCGAGGAG AGTCGACAGT GCTAAGCTTT
    GTGAGCTGGC TAACACTAAG TGGTACTGAG CAGTCTAGTG TGCGGGGCCC ATCCACTGAG
    AACCAAGAGG CCAAAAGAGC GGCCTTGGAC TGCATCAAGC ATTGTGACCC AGAAAAGATG
    ATCACAGAAA GCAAGTTCCT TCAGCTGGAG TCACTGCAGG AGCTCATGAA GGCTCTGGTC
    TCAGTGACGC CAGATGAAGA GACATATGAT GAGGAGGATG CTGCTTTCTG CCTAGAGATG
    CTGCTGAGGA TTGTGCTGGA GAACAGGGAC CGTGTGGGTT GTGTGTGGCA GACTGTCCGA
    GACCATCTAT ATCACCTGTG TGTCCAGGCA CAGGATTTCT GCTTCCTTGT GGAGCGGGCA
    GTGGTGGGGC TGCTCCGCTT GGCCATTCGA CTACTCCGAA GAGAAGAGAT CAGTGGCCAG
    GTGCTGCTCT CCCTGCGTAT TTTGCTACTA ATGAAGCCCA GCGTGCTGTC CCGAGTCAGT
    CACCAGGTAG CCTATGGGCT CCATGAACTT CTTAAGACCA ACGCAGCCAA CATCCACTCA
    GGTGACGACT GGGCCACTCT CTTCACACTG CTGGAATGCA TTGGCTCAGG TGTAAAGCCT
    CCAGCTGCCC TGCAGGCCAC AGCCAGGGCT GAAGCACCTG ATGCCGGGGC CCAATCAGAC
    AGCGAACTCC CATCCTACCA TCAGAATGAT GTGAGCCTGG ACCGAGGATA CACTTCTGAC
    TCGGAGGTCT ATGCTGACCA TTGCAGGCCT GGCAAGATCC ACCGATCAGC CACTGATGCT
    GATGTAGTCA ACAGCGGCTG GTTAGTGGTG GGGAAGGATG ACATTGATAA CTCCAAGCCA
    GGTCCTGGGC CCAGCCGGCT AGGCCCTTCA CCCCTGGTCA ATCAGTACAG CCTAACAGTG
    GGGCTGGACC TCGGGCCACA CGACACTAAG TCCCTGCTTA AATGTGTAGA ATCTCTGTCC
    TTCATTGTGC GTGATGCTGC CCACATTACA CCTGACAACT TTGAGCTCTG CGTGAAGACT
    CTCCGTATCT TTGTGGAGGC CAGTCTGAAT GGCGGGTGCA AGTCCCAGGA GAAACGTGGC
    AAGAGTCACA AATATGACAG CAAAGGAAAC CGCTTCAAGA AGAAATCCAA GGAAGGTTCA
    GTGCTTCGGC GGCCTCGAAC CTCTGGCCAA CATGCCACTC GGGGTGGGCA TAGTGACGAT
    GATGAGGATG AAGGGGTGCC TGCCAGCTAC CATACGGTGT CTTTACAGTT GTTAGATCTG
    ATGCACACCC TGCACACGCG AGCAGCCTCT ATCTACAGCT CTTGGGCGGA GGAACAGCGC
    CACCTAGAGA CAGGTGGCCG GAAGATAGAA GCGGATTCAC GTACCCTCTG GGCCCACTGC
    TGGTGTCCCT TACTGCAGGG CATTGCCTGC TTGTGCTGTG ATGCCCGGCG CCAGGTGCGG
    ATGCAAGCAC TGACTTATCT GCAACGAGCA CTACTGGTAC ATGATCTGCA GAAGCTAGAT
    GCCCTGGAGT GGGAGTCCTG TTTTAACAAG GTGCTGTTTC CTCTACTGAC CAAACTCTTG
    GAGAACATCA GCCCTGCAGA TGTGGGTGGG ATGGAGGAGA CTCGAATGAG GGCTTCCACA
    CTGCTGTCTA AGGTCTTCCT GCAGCACCTG TCTCCACTGC TGTCACTCTC TACCTTTGCG
    GCCCTCTGGC TGACCATCCT GGACTTCATG GACAAGTATA TGCATGCAGG ATCCAGTGAC
    TTACTGTCAG AGGCCATCCC TGAGTCTCTG AAGAACATGC TCCTGGTGAT GGACACAGCA
    GAGATTTTCC ACAATGCAGA TGCCCGAGGA GGCAGCCCCT CAGCCCTCTG GGAGATCACC
    TGGGAGCGCA TTGACTGTTT TCTGCCCCAC CTACGAGATG AGCTCTTCAA GCAGACTGTC
    ATCCAGGACC CCATGCCACT GGAGCCACAT GCCCAAAAAA CTCTGGCCTC AGCCCACATG
    ACTCCTGCTG CTGGAGACAC AAGGACACCT GGCCATCCAC CTCCCCCAGA GATGCCTTCT
    GAGCTGGGGG CCTGTGACTT CGAGAAGCCT GAGGGCCCTC GACCTGCCAA CAGCAGCTCT
    CCTGGATCAC CAGTGGCATC TAGCCCCAAC AGACTGAGCC CTACTTCAGA TGGGCCTCCT
    CCTCTGGCTC AGCCTCCACT GATCCTGCAG CCCTTGGCCT CCCCATTGCA GGTGGGCGTA
    CCACCCATGA CTCTACCAAT CATCCTCAAC CCTGCTCTCA TTGAGGCCAC TTCACCAGTG
    CCCCTCCTGG CCACACCCCG CCCCACAGAC CCAATGCCCA CCTCCGAGGT CAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_543987.2
    CDS269..5845
    Translation

    Target ORF information:

    RefSeq Version XM_543987.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_543987.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGATA AAAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCAATC 
    AAACGAAATG CCCGATGGAG CACTCATACT CCACTGGATG AAGAACGGGA TCCTCTGCTG
    CATAGTTTCA GTAATCTAAA GGAGGTTTTA AACAATATAA CAGAACTCTC CGAAATTGAA
    CCCAATGTGT TCCTCCGTCC ATTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT
    ATCACTGGAC TGGCACTTAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC
    ACTCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCACGCCCGT
    TTTGTGGGCA CAGATCCTGC CAGCGATGAG GTTGTCCTGA TGAAAATCCT TCAGGTGCTT
    CGGACTCTGT TGCTGACCCC AGCGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT
    ATGCAGTCTT GTTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC
    GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA
    GAAGAACCCA AGAACTATAT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCAGGAGGC
    ATGAGTGATT CATCCAAGTG GAAGAAACAG AAGAGATCCC CCCGGCCCCT GCGCCATATG
    ACCAAAGTAA CATCAGGTTC AGAGTTGCCC ACCTCCAATG GAACCACCTT ATCCTCTAAC
    CTCACTGGGG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA
    GCTGCCTCAA CGGTGGTCAG CCCCTCTACA GACAGTGGCC TGGAGTTCTC CTCTCAGACC
    ACCTCCAAGG AGGACCTCAC TGACCTGGAG CAACCTGGCT CTCCAGGGTA TAGCACAGCT
    ACAGAGCCTG GTAGCAGTGA GCTAGGGGTT CCTGAGCAGC CTGACCCGCA GCAGGAAGGA
    GTCCATGTGG AAAAGGCCCA GTCAGCATCT GTGGAATCCA TCCCCGAAGT GTTAGAGGAG
    TGCACGTCTC CTGCTGACCA CTCTGACTCT GCCTCTGTCC ATGACATGGA TTACGTCAAT
    CCCCGGGGTG TGCGCTTTAC ACAGTCCTCC CAGAAAGAAG GTACAGCTTT GGTTCCCTAT
    GGTCTTCCCT GCATCCGTGA GCTCTTCCGC TTCCTCATCT CCCTCACCAA TCCACATGAC
    CGCCACAATT CAGAAGTTAT GATCCACATG GGACTGCATT TGCTGACAGT GGCTCTTGAA
    TCTGCCCCTG TAGCCCAGTG CCAAACCCTT TTGGGCCTCA TCAAAGATGA GATGTGCCGC
    CACTTACTCC AGCTACTCAG TGTAGAGCGA CTCAACCTTT ATGCTGCTTC CCTGCGGGTA
    TGCTTCCTGC TTTTTGAGAG CATGAGGGAA CATCTCAAGT TCCAATTGGA GATGTACATC
    AAAAAGCTCA TGGAGATCAT CACTGTGGAG AACCCCAAAA TGCCTTATGA GATGAAAGAG
    ATGGCACTGG AGGCCATTGT GCAGCTCTGG CACATCCCCA GTTTTGTCAC TGAGCTCTAT
    ATCAACTATG ATTGTGACTA CTACTGTTCA AACCTCTTTG AAGACCTCAC CAAGCTGCTG
    TCCAAGAATG CCTTCCCTGT GTCTGGTCAG CTCTATACAA CACACCTCCT GTCTCTTGAT
    GCCCTGTTGA CAGTGATTGA CAGCACTGAG GCCCATTGCC AGGCCAAAGT CCTCAACAGT
    CTTACCCAGC AAGAGAAGAA AGAAGCAGCC AGATCTGGCT ATGAGGCGGT GGATGGTACC
    CGAGAATCCA GCAATAGTGA GAGAGCAGCC AGCGATGGGA AACCTGTAGG CATAGCCGCA
    GACATCCCAG GTCTACATCT GCCAGGTGGA GGGCGGCTGC CAGCAGAACA TGGGAAGCCA
    GGATGCAGTG ATCTGGAGGA AGCTGCTGAC TCTGGGGCTG ACAAAAAGTT TACCCGGAAG
    CCACCTCGAT TTTCCTGTCT CCTGCCAGAT CCACGGGAAC TGATTGAAAT TAAAAACAAA
    AAGAAGCTGC TAATCACTGG CACAGAGCAG TTCAACCAGA AACCAAAGAA GGGAATCCAG
    TTTCTGCAGG AGAAAGGCCT CCTCACCATC CCAATGGACA ACACAGAGGT AGCCCAGTGG
    CTCCGAGAGA ACCCTCGGCT GGACAAGAAA ATGATTGGAG AGTTTGTGAG TGACCGCAAA
    AACATTGACC TGTTGGAGAG CTTTGTGAGC ACCTTCAGCT TTCAGGGTCT GCGGCTGGAT
    GAAGCTCTTC GTCTCTACCT GGAAGCCTTT CGCTTACCTG GGGAAGCACC AGTCATCCAG
    AGGTTGCTGG AGGCATTCAC AGAGCATTGG AGGAATTGTA ATGGCTCCCC ATTTGCCAAT
    AGCGATGCCT GCTTTGCTCT GGCCTATGCT GTCATCTTGC TTAATACTGA CCAGCACAAC
    CACAACGTTC GCAAACAGAA TGCACCCATG ACTCTAGAGG AGTTTCGCAA AAACCTAAAA
    GGTGTGAATG GAGGCAAGGA CTTTGAGCAA GACATCCTGG AGGACATGTA CCATGCCATC
    AAGAATGAGG AAATTGTGAT GCCTGAAGAG CAGACAGGCT TGGTTCGGGA GAACTATGTG
    TGGAATGTGC TACTTCATCG AGGTGCCACC CCAGAGGGCA TATTCCTTCG TGTGCCTGCT
    GGCAGCTATG ATCTTGACCT CTTCACCATG ACGTGGGGCC CCACTATTGC TGCCCTTTCT
    TATGTCTTTG ACAAAAGCCT TGAGGAAACA ATTATCCAGA AAGCCATCTC AGGCTTCAGG
    AAGTGCGCCA TGATCTCCGC CCACTATGGC CTCAGCGATG TGTTTGACAA TCTCATCATA
    TCTCTGTGCA AATTCACAGC TCTTAGCAGT GAGTCTATTG AGAATCTGCC CAGTGTGTTT
    GGAAGCAACC CCAAAGCCCA CATTGCAGCC AAGACAGTAT TCCATTTGGC CCATCGTCAT
    GGTGATATCC TGCGGGAGGG CTGGAAGAAT ATCATGGAAG CCATGCTGCA GCTCTTCCGA
    GCCCAACTGC TTCCCAAAGC TATGGTGGAG GTAGAAGATT TTGTGGATCC CAATGGCAAG
    ATCTCTCTAC AGAGGGAGGA GACACCATCA AACCGAGGAG AGTCGACAGT GCTAAGCTTT
    GTGAGCTGGC TAACACTAAG TGGTACTGAG CAGTCTAGTG TGCGGGGCCC ATCCACTGAG
    AACCAAGAGG CCAAAAGAGC GGCCTTGGAC TGCATCAAGC ATTGTGACCC AGAAAAGATG
    ATCACAGAAA GCAAGTTCCT TCAGCTGGAG TCACTGCAGG AGCTCATGAA GGCTCTGGTC
    TCAGTGACGC CAGATGAAGA GACATATGAT GAGGAGGATG CTGCTTTCTG CCTAGAGATG
    CTGCTGAGGA TTGTGCTGGA GAACAGGGAC CGTGTGGGTT GTGTGTGGCA GACTGTCCGA
    GACCATCTAT ATCACCTGTG TGTCCAGGCA CAGGATTTCT GCTTCCTTGT GGAGCGGGCA
    GTGGTGGGGC TGCTCCGCTT GGCCATTCGA CTACTCCGAA GAGAAGAGAT CAGTGGCCAG
    GTGCTGCTCT CCCTGCGTAT TTTGCTACTA ATGAAGCCCA GCGTGCTGTC CCGAGTCAGT
    CACCAGGTAG CCTATGGGCT CCATGAACTT CTTAAGACCA ACGCAGCCAA CATCCACTCA
    GGTGACGACT GGGCCACTCT CTTCACACTG CTGGAATGCA TTGGCTCAGG TGTAAAGCCT
    CCAGCTGCCC TGCAGGCCAC AGCCAGGGCT GAAGCACCTG ATGCCGGGGC CCAATCAGAC
    AGCGAACTCC CATCCTACCA TCAGAATGAT GTGAGCCTGG ACCGAGGATA CACTTCTGAC
    TCGGAGGTCT ATGCTGACCA TTGCAGGCCT GGCAAGATCC ACCGATCAGC CACTGATGCT
    GATGTAGTCA ACAGCGGCTG GTTAGTGGTG GGGAAGGATG ACATTGATAA CTCCAAGCCA
    GGTCCTGGGC CCAGCCGGCT AGGCCCTTCA CCCCTGGTCA ATCAGTACAG CCTAACAGTG
    GGGCTGGACC TCGGGCCACA CGACACTAAG TCCCTGCTTA AATGTGTAGA ATCTCTGTCC
    TTCATTGTGC GTGATGCTGC CCACATTACA CCTGACAACT TTGAGCTCTG CGTGAAGACT
    CTCCGTATCT TTGTGGAGGC CAGTCTGAAT GGCGGGTGCA AGTCCCAGGA GAAACGTGGC
    AAGAGTCACA AATATGACAG CAAAGGAAAC CGCTTCAAGA AGAAATCCAA GGAAGGTTCA
    GTGCTTCGGC GGCCTCGAAC CTCTGGCCAA CATGCCACTC GGGGTGGGCA TAGTGACGAT
    GATGAGGATG AAGGGGTGCC TGCCAGCTAC CATACGGTGT CTTTACAGTT GTTAGATCTG
    ATGCACACCC TGCACACGCG AGCAGCCTCT ATCTACAGCT CTTGGGCGGA GGAACAGCGC
    CACCTAGAGA CAGGTGGCCG GAAGATAGAA GCGGATTCAC GTACCCTCTG GGCCCACTGC
    TGGTGTCCCT TACTGCAGGG CATTGCCTGC TTGTGCTGTG ATGCCCGGCG CCAGGTGCGG
    ATGCAAGCAC TGACTTATCT GCAACGAGCA CTACTGGTAC ATGATCTGCA GAAGCTAGAT
    GCCCTGGAGT GGGAGTCCTG TTTTAACAAG GTGCTGTTTC CTCTACTGAC CAAACTCTTG
    GAGAACATCA GCCCTGCAGA TGTGGGTGGG ATGGAGGAGA CTCGAATGAG GGCTTCCACA
    CTGCTGTCTA AGGTCTTCCT GCAGCACCTG TCTCCACTGC TGTCACTCTC TACCTTTGCG
    GCCCTCTGGC TGACCATCCT GGACTTCATG GACAAGTATA TGCATGCAGG ATCCAGTGAC
    TTACTGTCAG AGGCCATCCC TGAGTCTCTG AAGAACATGC TCCTGGTGAT GGACACAGCA
    GAGATTTTCC ACAATGCAGA TGCCCGAGGA GGCAGCCCCT CAGCCCTCTG GGAGATCACC
    TGGGAGCGCA TTGACTGTTT TCTGCCCCAC CTACGAGATG AGCTCTTCAA GCAGACTGTC
    ATCCAGGACC CCATGCCACT GGAGCCACAT GCCCAAAAAA CTCTGGCCTC AGCCCACATG
    ACTCCTGCTG CTGGAGACAC AAGGACACCT GGCCATCCAC CTCCCCCAGA GATGCCTTCT
    GAGCTGGGGG CCTGTGACTT CGAGAAGCCT GAGGGCCCTC GACCTGCCAA CAGCAGCTCT
    CCTGGATCAC CAGTGGCATC TAGCCCCAAC AGACTGAGCC CTACTTCAGA TGGGCCTCCT
    CCTCTGGCTC AGCCTCCACT GATCCTGCAG CCCTTGGCCT CCCCATTGCA GGTGGGCGTA
    CCACCCATGA CTCTACCAAT CATCCTCAAC CCTGCTCTCA TTGAGGCCAC TTCACCAGTG
    CCCCTCCTGG CCACACCCCG CCCCACAGAC CCAATGCCCA CCTCCGAGGT CAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.