HECTD2 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	HECTD2
Entrez Gene ID	486795
Full Name	HECT domain E3 ubiquitin protein ligase 2
General protein information	Preferred Names HECT domain E3 ubiquitin protein ligase 2 Names probable E3 ubiquitin-protein ligase HECTD2 HECT domain containing 2
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014109018.2	XP_013964493.1	probable E3 ubiquitin-protein ligase HECTD2 isoform X1
XM_014109019.1	XP_013964494.1	probable E3 ubiquitin-protein ligase HECTD2 isoform X2
XM_014109020.1	XP_013964495.1	probable E3 ubiquitin-protein ligase HECTD2 isoform X3
XM_014109020.2	XP_013964495.1	probable E3 ubiquitin-protein ligase HECTD2 isoform X3
XM_014109018.1	XP_013964493.1	probable E3 ubiquitin-protein ligase HECTD2 isoform X1
XM_014109019.2	XP_013964494.1	probable E3 ubiquitin-protein ligase HECTD2 isoform X2

Pathways from BioSystems

Homologies

	HECTD2	XP_001088322.1
Gallus gallus (chicken)	HECTD2	XP_004942207.1
Xenopus tropicalis (tropical clawed frog)	hectd2	NP_001106408.1
Pan troglodytes (chimpanzee)	HECTD2	XP_003312725.1
Homo sapiens (human)	HECTD2	NP_877497.3
Canis lupus familiaris (dog)	HECTD2	XP_543924.2
Bos taurus (cattle)	HECTD2	NP_001193701.1
Rattus norvegicus (Norway rat)	Hectd2	NP_001101078.2
Mus musculus (house mouse)	Hectd2	NP_001156943.1
Danio rerio (zebrafish)	hectd2	XP_691455.5

Gene Ontology

The following HECTD2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HECTD2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe43980	XM_014109018.2 Latest version!	Canis lupus familiaris HECT domain E3 ubiquitin protein ligase 2 (HECTD2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCe43981	XM_014109019.2 Latest version!	Canis lupus familiaris HECT domain E3 ubiquitin protein ligase 2 (HECTD2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCe43982	XM_014109020.2 Latest version!	Canis lupus familiaris HECT domain E3 ubiquitin protein ligase 2 (HECTD2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
OCe43982	XM_014109020.1	Canis lupus familiaris HECT domain containing E3 ubiquitin protein ligase 2 (HECTD2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OCe43980	XM_014109018.1	Canis lupus familiaris HECT domain containing E3 ubiquitin protein ligase 2 (HECTD2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCe43981	XM_014109019.1	Canis lupus familiaris HECT domain containing E3 ubiquitin protein ligase 2 (HECTD2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HECTD2 ( XM_014109018.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013964493.1 Canis lupus familiaris HECT domain E3 ubiquitin protein ligase 2 (HECTD2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe43980
Clone ID Related Accession (Same CDS sequence)	XM_014109018.2 , XM_014109018.1
Accession Version	XM_014109018.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	probable E3 ubiquitin-protein ligase HECTD2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006610.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109018.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAAG AGGGCCCGTT GAGGGCGCCC GCCGGGCCGC CCGAGCTGGC GGCGGCCTCT 
TCGGAGGACA GGAAGGGAAG GGAGCCGGAG CGCGAGAAGC TGCCGCCCAT CGTGTCGGCG 
GGCGCCGGAG CGGCTGCGGC GGGTTTGGAC AGAGGATCCA AAGGTCAAAC TTCCACTTTT 
AGCAGTTTTA TTTCAACTGT TAACCAGAAG AAAGAAGCTG CTGAAAACAG AAGTTCACCT 
ACACATCTCA TTTTCCCTAA CATCAAGAAT GTGAGAGACC TACCATCAAT TTGCCTTGAT 
GTTAGACAAA AACAGCGCAT TTCTGTAGAC CCATTATCAT CTGAAATAAA GGCCCCAGTT 
CTTCCAGAAC CTGTCCTTCC TCGCCAGCCC AAAACTATGA AAGACTTTCA GGAAGATGTA 
GAAAAAGCTA AGTCAACAGG AGATTGGAAA GCAGTACATG ATTTTTATCT AACAACATTT 
GATTCTTTCC CAGAACTAAA TGCTGCATTT AAGAAAGATG CCACCGCCTC ATTTAATACC 
ATTGAAGATT CTGGGATTAA TGCTAAATTT GTGAATGCTG TGTATGATGC CTTACTTAAT 
ACTCCTCAAG ACATTCAGAA GACAGTATTA AAGGGAATCA TTAACAGCTT ATTACGAGAA 
TGGAAAGGTC CACGAACAAA AGATGATCTT AGAGCATATT TTATACTTTT ACAGAATCCT 
CAATTTAATA ATACATCTAC GTATGTCATC TATGCTCACT TGCTACGGCA GATAGCTACC 
TTAGTGGAAG CTGATCATCA TTTCCTAGTT CACTGGTTTA AAAAAATATC CCAGAAGAGG 
TTCAAACAAT TGGTAGAGAG ATTGCTGCAG TTTATTTCTT TGCGCCTGTT TCCTGCAAAG 
CCTGAAGAAT TTCCACCTAT CACAAAGTGT TCCTGGTGGA TCCCATCAGC ATCTAAAGTG 
TTGGCTTTAC TTAATACTGC AAACAGTTTG ATTCATCCTT CCCTTATTCC TTATACTGAT 
TTCTATAATT CTACATTGGA TCACATTGAT CTTATGGAAG AATACCACAC CTGGCAGAGC 
TTTGGAAACT CTCACAGGTT TTCCTTCTGT CAGTACCCAT TCGTTATTTC TATAGCTGCA 
AAAAAAATCA TTATTCAAAG AGACTCAGAA CAACAGATGA TAAGCATCGC AAGGCAAAGT 
CTGGTGGATA AAGTATCTCG AAGACAGAGA CCTGATATGA ACATGTTATT TCTAAATATG 
AAAGTAAGAC GGACACATTT GGTTAGTGAC TCGCTTGATG AGTTAACCCG GAAAAGAGCA 
GACTTAAAAA AGAAGTTGAA AGTTACATTT GTAGGGGAAG CTGGTTTGGA CATGGGTGGC 
TTGACTAAAG AATGGTTCCT TCTTCTAATT CGCCAAATTT TTCATCCAGA TTATGGCATG 
TTTACATATC ACAAGGATTC ACACTGCCAT TGGTTTAGCA GTTTTAAATG TGATAACTAT 
TCTGAATTCC GATTGGTTGG AATTCTTATG GGACTAGCTG TGTATAACAG CATCACCTTG 
GATATTCGTT TCCCTCCCTG CTGTTACAAG AAATTATTGA GCCCTCCCAT CATTCCTAGT 
GATCAAAATA TACCAGTAGG CATCTGCAGT GTGACCACTG ACGACTTATG TCAAATTATG 
CCTGAGTTGG CCCATGGGTT AAGTGAACTC TTGTCGTATG AAGGCAATGT TGAAGAAGAT 
TTCTATTCAA CATTCCAGGT TTTTCAAGAA GAATTTGGAA TAATTAAGTC CTACAATTTA 
AAGCCAGGTG GTGATAAAAT TCCAGTTACC AATCAAAATA GGAAAGAATA TGTACAGCTT 
TATATTGACT TTCTTCTCAA CAAATCCATC TATAAGCAGT TTGCTGCATT TTATTATGGA 
TTTCATAGTG TGTGTGCTTC AAATGCCCTG ATGCTGCTTC GTCCAGAAGA GGTTGAAATT 
CTGGTATGTG GAAGCCCTGA ACTGGATATG TATGCTCTAC AAAGAAGTAC TCAGTATGAT 
GGCTATGCCA AAACAGACTT AACTATAAGA TATTTTTGGG ATGTTGTGCT TGGATTTCCT 
CTTGATCTTC AAAAGAAGTT GCTACATTTT ACTACAGGAA GTGACAGAGT ACCGGTAGGA 
GGAATGGCTG ACTTGAACTT TAAAATCTCA AAGAATGAAA CTTCTACTAA CTGGTTGCCT 
GTGGCCCACA CCTGCTTCAA TCAACTTTGC CTTCCCCCCT ACAAGAGCAA AAAGGACCTA 
AAACAGAAAT TGATCATTGG AATTTCAAAT TCAGAAGGTT TTGGACTTGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013964493.1
CDS	1..2334
Translation	MSEEGPLRAPAGPPELAAASSEDRKGREPEREKLPPIVSAGAGAAAAGLDRGSKGQTSTFSSFISTVNQKKEAAENRSSPTHLIFPNIKNVRDLPSICLDVRQKQRISVDPLSSEIKAPVLPEPVLPRQPKTMKDFQEDVEKAKSTGDWKAVHDFYLTTFDSFPELNAAFKKDATASFNTIEDSGINAKFVNAVYDALLNTPQDIQKTVLKGIINSLLREWKGPRTKDDLRAYFILLQNPQFNNTSTYVIYAHLLRQIATLVEADHHFLVHWFKKISQKRFKQLVERLLQFISLRLFPAKPEEFPPITKCSWWIPSASKVLALLNTANSLIHPSLIPYTDFYNSTLDHIDLMEEYHTWQSFGNSHRFSFCQYPFVISIAAKKIIIQRDSEQQMISIARQSLVDKVSRRQRPDMNMLFLNMKVRRTHLVSDSLDELTRKRADLKKKLKVTFVGEAGLDMGGLTKEWFLLLIRQIFHPDYGMFTYHKDSHCHWFSSFKCDNYSEFRLVGILMGLAVYNSITLDIRFPPCCYKKLLSPPIIPSDQNIPVGICSVTTDDLCQIMPELAHGLSELLSYEGNVEEDFYSTFQVFQEEFGIIKSYNLKPGGDKIPVTNQNRKEYVQLYIDFLLNKSIYKQFAAFYYGFHSVCASNALMLLRPEEVEILVCGSPELDMYALQRSTQYDGYAKTDLTIRYFWDVVLGFPLDLQKKLLHFTTGSDRVPVGGMADLNFKISKNETSTNWLPVAHTCFNQLCLPPYKSKKDLKQKLIIGISNSEGFGLE

Target ORF information:

RefSeq Version	XM_014109018.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris HECT domain E3 ubiquitin protein ligase 2 (HECTD2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109018.2

ORF Insert Sequence:

ATGAGTGAAG AGGGCCCGTT GAGGGCGCCC GCCGGGCCGC CCGAGCTGGC GGCGGCCTCT 
TCGGAGGACA GGAAGGGAAG GGAGCCGGAG CGCGAGAAGC TGCCGCCCAT CGTGTCGGCG 
GGCGCCGGAG CGGCTGCGGC GGGTTTGGAC AGAGGATCCA AAGGTCAAAC TTCCACTTTT 
AGCAGTTTTA TTTCAACTGT TAACCAGAAG AAAGAAGCTG CTGAAAACAG AAGTTCACCT 
ACACATCTCA TTTTCCCTAA CATCAAGAAT GTGAGAGACC TACCATCAAT TTGCCTTGAT 
GTTAGACAAA AACAGCGCAT TTCTGTAGAC CCATTATCAT CTGAAATAAA GGCCCCAGTT 
CTTCCAGAAC CTGTCCTTCC TCGCCAGCCC AAAACTATGA AAGACTTTCA GGAAGATGTA 
GAAAAAGCTA AGTCAACAGG AGATTGGAAA GCAGTACATG ATTTTTATCT AACAACATTT 
GATTCTTTCC CAGAACTAAA TGCTGCATTT AAGAAAGATG CCACCGCCTC ATTTAATACC 
ATTGAAGATT CTGGGATTAA TGCTAAATTT GTGAATGCTG TGTATGATGC CTTACTTAAT 
ACTCCTCAAG ACATTCAGAA GACAGTATTA AAGGGAATCA TTAACAGCTT ATTACGAGAA 
TGGAAAGGTC CACGAACAAA AGATGATCTT AGAGCATATT TTATACTTTT ACAGAATCCT 
CAATTTAATA ATACATCTAC GTATGTCATC TATGCTCACT TGCTACGGCA GATAGCTACC 
TTAGTGGAAG CTGATCATCA TTTCCTAGTT CACTGGTTTA AAAAAATATC CCAGAAGAGG 
TTCAAACAAT TGGTAGAGAG ATTGCTGCAG TTTATTTCTT TGCGCCTGTT TCCTGCAAAG 
CCTGAAGAAT TTCCACCTAT CACAAAGTGT TCCTGGTGGA TCCCATCAGC ATCTAAAGTG 
TTGGCTTTAC TTAATACTGC AAACAGTTTG ATTCATCCTT CCCTTATTCC TTATACTGAT 
TTCTATAATT CTACATTGGA TCACATTGAT CTTATGGAAG AATACCACAC CTGGCAGAGC 
TTTGGAAACT CTCACAGGTT TTCCTTCTGT CAGTACCCAT TCGTTATTTC TATAGCTGCA 
AAAAAAATCA TTATTCAAAG AGACTCAGAA CAACAGATGA TAAGCATCGC AAGGCAAAGT 
CTGGTGGATA AAGTATCTCG AAGACAGAGA CCTGATATGA ACATGTTATT TCTAAATATG 
AAAGTAAGAC GGACACATTT GGTTAGTGAC TCGCTTGATG AGTTAACCCG GAAAAGAGCA 
GACTTAAAAA AGAAGTTGAA AGTTACATTT GTAGGGGAAG CTGGTTTGGA CATGGGTGGC 
TTGACTAAAG AATGGTTCCT TCTTCTAATT CGCCAAATTT TTCATCCAGA TTATGGCATG 
TTTACATATC ACAAGGATTC ACACTGCCAT TGGTTTAGCA GTTTTAAATG TGATAACTAT 
TCTGAATTCC GATTGGTTGG AATTCTTATG GGACTAGCTG TGTATAACAG CATCACCTTG 
GATATTCGTT TCCCTCCCTG CTGTTACAAG AAATTATTGA GCCCTCCCAT CATTCCTAGT 
GATCAAAATA TACCAGTAGG CATCTGCAGT GTGACCACTG ACGACTTATG TCAAATTATG 
CCTGAGTTGG CCCATGGGTT AAGTGAACTC TTGTCGTATG AAGGCAATGT TGAAGAAGAT 
TTCTATTCAA CATTCCAGGT TTTTCAAGAA GAATTTGGAA TAATTAAGTC CTACAATTTA 
AAGCCAGGTG GTGATAAAAT TCCAGTTACC AATCAAAATA GGAAAGAATA TGTACAGCTT 
TATATTGACT TTCTTCTCAA CAAATCCATC TATAAGCAGT TTGCTGCATT TTATTATGGA 
TTTCATAGTG TGTGTGCTTC AAATGCCCTG ATGCTGCTTC GTCCAGAAGA GGTTGAAATT 
CTGGTATGTG GAAGCCCTGA ACTGGATATG TATGCTCTAC AAAGAAGTAC TCAGTATGAT 
GGCTATGCCA AAACAGACTT AACTATAAGA TATTTTTGGG ATGTTGTGCT TGGATTTCCT 
CTTGATCTTC AAAAGAAGTT GCTACATTTT ACTACAGGAA GTGACAGAGT ACCGGTAGGA 
GGAATGGCTG ACTTGAACTT TAAAATCTCA AAGAATGAAA CTTCTACTAA CTGGTTGCCT 
GTGGCCCACA CCTGCTTCAA TCAACTTTGC CTTCCCCCCT ACAAGAGCAA AAAGGACCTA 
AAACAGAAAT TGATCATTGG AATTTCAAAT TCAGAAGGTT TTGGACTTGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECTD2 ( XM_014109018.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013964493.1 Canis lupus familiaris HECT domain containing E3 ubiquitin protein ligase 2 (HECTD2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe43980
Clone ID Related Accession (Same CDS sequence)	XM_014109018.2 , XM_014109018.1
Accession Version	XM_014109018.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	probable E3 ubiquitin-protein ligase HECTD2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAAG AGGGCCCGTT GAGGGCGCCC GCCGGGCCGC CCGAGCTGGC GGCGGCCTCT 
TCGGAGGACA GGAAGGGAAG GGAGCCGGAG CGCGAGAAGC TGCCGCCCAT CGTGTCGGCG 
GGCGCCGGAG CGGCTGCGGC GGGTTTGGAC AGAGGATCCA AAGGTCAAAC TTCCACTTTT 
AGCAGTTTTA TTTCAACTGT TAACCAGAAG AAAGAAGCTG CTGAAAACAG AAGTTCACCT 
ACACATCTCA TTTTCCCTAA CATCAAGAAT GTGAGAGACC TACCATCAAT TTGCCTTGAT 
GTTAGACAAA AACAGCGCAT TTCTGTAGAC CCATTATCAT CTGAAATAAA GGCCCCAGTT 
CTTCCAGAAC CTGTCCTTCC TCGCCAGCCC AAAACTATGA AAGACTTTCA GGAAGATGTA 
GAAAAAGCTA AGTCAACAGG AGATTGGAAA GCAGTACATG ATTTTTATCT AACAACATTT 
GATTCTTTCC CAGAACTAAA TGCTGCATTT AAGAAAGATG CCACCGCCTC ATTTAATACC 
ATTGAAGATT CTGGGATTAA TGCTAAATTT GTGAATGCTG TGTATGATGC CTTACTTAAT 
ACTCCTCAAG ACATTCAGAA GACAGTATTA AAGGGAATCA TTAACAGCTT ATTACGAGAA 
TGGAAAGGTC CACGAACAAA AGATGATCTT AGAGCATATT TTATACTTTT ACAGAATCCT 
CAATTTAATA ATACATCTAC GTATGTCATC TATGCTCACT TGCTACGGCA GATAGCTACC 
TTAGTGGAAG CTGATCATCA TTTCCTAGTT CACTGGTTTA AAAAAATATC CCAGAAGAGG 
TTCAAACAAT TGGTAGAGAG ATTGCTGCAG TTTATTTCTT TGCGCCTGTT TCCTGCAAAG 
CCTGAAGAAT TTCCACCTAT CACAAAGTGT TCCTGGTGGA TCCCATCAGC ATCTAAAGTG 
TTGGCTTTAC TTAATACTGC AAACAGTTTG ATTCATCCTT CCCTTATTCC TTATACTGAT 
TTCTATAATT CTACATTGGA TCACATTGAT CTTATGGAAG AATACCACAC CTGGCAGAGC 
TTTGGAAACT CTCACAGGTT TTCCTTCTGT CAGTACCCAT TCGTTATTTC TATAGCTGCA 
AAAAAAATCA TTATTCAAAG AGACTCAGAA CAACAGATGA TAAGCATCGC AAGGCAAAGT 
CTGGTGGATA AAGTATCTCG AAGACAGAGA CCTGATATGA ACATGTTATT TCTAAATATG 
AAAGTAAGAC GGACACATTT GGTTAGTGAC TCGCTTGATG AGTTAACCCG GAAAAGAGCA 
GACTTAAAAA AGAAGTTGAA AGTTACATTT GTAGGGGAAG CTGGTTTGGA CATGGGTGGC 
TTGACTAAAG AATGGTTCCT TCTTCTAATT CGCCAAATTT TTCATCCAGA TTATGGCATG 
TTTACATATC ACAAGGATTC ACACTGCCAT TGGTTTAGCA GTTTTAAATG TGATAACTAT 
TCTGAATTCC GATTGGTTGG AATTCTTATG GGACTAGCTG TGTATAACAG CATCACCTTG 
GATATTCGTT TCCCTCCCTG CTGTTACAAG AAATTATTGA GCCCTCCCAT CATTCCTAGT 
GATCAAAATA TACCAGTAGG CATCTGCAGT GTGACCACTG ACGACTTATG TCAAATTATG 
CCTGAGTTGG CCCATGGGTT AAGTGAACTC TTGTCGTATG AAGGCAATGT TGAAGAAGAT 
TTCTATTCAA CATTCCAGGT TTTTCAAGAA GAATTTGGAA TAATTAAGTC CTACAATTTA 
AAGCCAGGTG GTGATAAAAT TCCAGTTACC AATCAAAATA GGAAAGAATA TGTACAGCTT 
TATATTGACT TTCTTCTCAA CAAATCCATC TATAAGCAGT TTGCTGCATT TTATTATGGA 
TTTCATAGTG TGTGTGCTTC AAATGCCCTG ATGCTGCTTC GTCCAGAAGA GGTTGAAATT 
CTGGTATGTG GAAGCCCTGA ACTGGATATG TATGCTCTAC AAAGAAGTAC TCAGTATGAT 
GGCTATGCCA AAACAGACTT AACTATAAGA TATTTTTGGG ATGTTGTGCT TGGATTTCCT 
CTTGATCTTC AAAAGAAGTT GCTACATTTT ACTACAGGAA GTGACAGAGT ACCGGTAGGA 
GGAATGGCTG ACTTGAACTT TAAAATCTCA AAGAATGAAA CTTCTACTAA CTGGTTGCCT 
GTGGCCCACA CCTGCTTCAA TCAACTTTGC CTTCCCCCCT ACAAGAGCAA AAAGGACCTA 
AAACAGAAAT TGATCATTGG AATTTCAAAT TCAGAAGGTT TTGGACTTGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013964493.1
CDS	1..2334
Translation	MSEEGPLRAPAGPPELAAASSEDRKGREPEREKLPPIVSAGAGAAAAGLDRGSKGQTSTFSSFISTVNQKKEAAENRSSPTHLIFPNIKNVRDLPSICLDVRQKQRISVDPLSSEIKAPVLPEPVLPRQPKTMKDFQEDVEKAKSTGDWKAVHDFYLTTFDSFPELNAAFKKDATASFNTIEDSGINAKFVNAVYDALLNTPQDIQKTVLKGIINSLLREWKGPRTKDDLRAYFILLQNPQFNNTSTYVIYAHLLRQIATLVEADHHFLVHWFKKISQKRFKQLVERLLQFISLRLFPAKPEEFPPITKCSWWIPSASKVLALLNTANSLIHPSLIPYTDFYNSTLDHIDLMEEYHTWQSFGNSHRFSFCQYPFVISIAAKKIIIQRDSEQQMISIARQSLVDKVSRRQRPDMNMLFLNMKVRRTHLVSDSLDELTRKRADLKKKLKVTFVGEAGLDMGGLTKEWFLLLIRQIFHPDYGMFTYHKDSHCHWFSSFKCDNYSEFRLVGILMGLAVYNSITLDIRFPPCCYKKLLSPPIIPSDQNIPVGICSVTTDDLCQIMPELAHGLSELLSYEGNVEEDFYSTFQVFQEEFGIIKSYNLKPGGDKIPVTNQNRKEYVQLYIDFLLNKSIYKQFAAFYYGFHSVCASNALMLLRPEEVEILVCGSPELDMYALQRSTQYDGYAKTDLTIRYFWDVVLGFPLDLQKKLLHFTTGSDRVPVGGMADLNFKISKNETSTNWLPVAHTCFNQLCLPPYKSKKDLKQKLIIGISNSEGFGLE

Target ORF information:

RefSeq Version	XM_014109018.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris HECT domain containing E3 ubiquitin protein ligase 2 (HECTD2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109018.1

ORF Insert Sequence:

ATGAGTGAAG AGGGCCCGTT GAGGGCGCCC GCCGGGCCGC CCGAGCTGGC GGCGGCCTCT 
TCGGAGGACA GGAAGGGAAG GGAGCCGGAG CGCGAGAAGC TGCCGCCCAT CGTGTCGGCG 
GGCGCCGGAG CGGCTGCGGC GGGTTTGGAC AGAGGATCCA AAGGTCAAAC TTCCACTTTT 
AGCAGTTTTA TTTCAACTGT TAACCAGAAG AAAGAAGCTG CTGAAAACAG AAGTTCACCT 
ACACATCTCA TTTTCCCTAA CATCAAGAAT GTGAGAGACC TACCATCAAT TTGCCTTGAT 
GTTAGACAAA AACAGCGCAT TTCTGTAGAC CCATTATCAT CTGAAATAAA GGCCCCAGTT 
CTTCCAGAAC CTGTCCTTCC TCGCCAGCCC AAAACTATGA AAGACTTTCA GGAAGATGTA 
GAAAAAGCTA AGTCAACAGG AGATTGGAAA GCAGTACATG ATTTTTATCT AACAACATTT 
GATTCTTTCC CAGAACTAAA TGCTGCATTT AAGAAAGATG CCACCGCCTC ATTTAATACC 
ATTGAAGATT CTGGGATTAA TGCTAAATTT GTGAATGCTG TGTATGATGC CTTACTTAAT 
ACTCCTCAAG ACATTCAGAA GACAGTATTA AAGGGAATCA TTAACAGCTT ATTACGAGAA 
TGGAAAGGTC CACGAACAAA AGATGATCTT AGAGCATATT TTATACTTTT ACAGAATCCT 
CAATTTAATA ATACATCTAC GTATGTCATC TATGCTCACT TGCTACGGCA GATAGCTACC 
TTAGTGGAAG CTGATCATCA TTTCCTAGTT CACTGGTTTA AAAAAATATC CCAGAAGAGG 
TTCAAACAAT TGGTAGAGAG ATTGCTGCAG TTTATTTCTT TGCGCCTGTT TCCTGCAAAG 
CCTGAAGAAT TTCCACCTAT CACAAAGTGT TCCTGGTGGA TCCCATCAGC ATCTAAAGTG 
TTGGCTTTAC TTAATACTGC AAACAGTTTG ATTCATCCTT CCCTTATTCC TTATACTGAT 
TTCTATAATT CTACATTGGA TCACATTGAT CTTATGGAAG AATACCACAC CTGGCAGAGC 
TTTGGAAACT CTCACAGGTT TTCCTTCTGT CAGTACCCAT TCGTTATTTC TATAGCTGCA 
AAAAAAATCA TTATTCAAAG AGACTCAGAA CAACAGATGA TAAGCATCGC AAGGCAAAGT 
CTGGTGGATA AAGTATCTCG AAGACAGAGA CCTGATATGA ACATGTTATT TCTAAATATG 
AAAGTAAGAC GGACACATTT GGTTAGTGAC TCGCTTGATG AGTTAACCCG GAAAAGAGCA 
GACTTAAAAA AGAAGTTGAA AGTTACATTT GTAGGGGAAG CTGGTTTGGA CATGGGTGGC 
TTGACTAAAG AATGGTTCCT TCTTCTAATT CGCCAAATTT TTCATCCAGA TTATGGCATG 
TTTACATATC ACAAGGATTC ACACTGCCAT TGGTTTAGCA GTTTTAAATG TGATAACTAT 
TCTGAATTCC GATTGGTTGG AATTCTTATG GGACTAGCTG TGTATAACAG CATCACCTTG 
GATATTCGTT TCCCTCCCTG CTGTTACAAG AAATTATTGA GCCCTCCCAT CATTCCTAGT 
GATCAAAATA TACCAGTAGG CATCTGCAGT GTGACCACTG ACGACTTATG TCAAATTATG 
CCTGAGTTGG CCCATGGGTT AAGTGAACTC TTGTCGTATG AAGGCAATGT TGAAGAAGAT 
TTCTATTCAA CATTCCAGGT TTTTCAAGAA GAATTTGGAA TAATTAAGTC CTACAATTTA 
AAGCCAGGTG GTGATAAAAT TCCAGTTACC AATCAAAATA GGAAAGAATA TGTACAGCTT 
TATATTGACT TTCTTCTCAA CAAATCCATC TATAAGCAGT TTGCTGCATT TTATTATGGA 
TTTCATAGTG TGTGTGCTTC AAATGCCCTG ATGCTGCTTC GTCCAGAAGA GGTTGAAATT 
CTGGTATGTG GAAGCCCTGA ACTGGATATG TATGCTCTAC AAAGAAGTAC TCAGTATGAT 
GGCTATGCCA AAACAGACTT AACTATAAGA TATTTTTGGG ATGTTGTGCT TGGATTTCCT 
CTTGATCTTC AAAAGAAGTT GCTACATTTT ACTACAGGAA GTGACAGAGT ACCGGTAGGA 
GGAATGGCTG ACTTGAACTT TAAAATCTCA AAGAATGAAA CTTCTACTAA CTGGTTGCCT 
GTGGCCCACA CCTGCTTCAA TCAACTTTGC CTTCCCCCCT ACAAGAGCAA AAAGGACCTA 
AAACAGAAAT TGATCATTGG AATTTCAAAT TCAGAAGGTT TTGGACTTGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECTD2 ( XM_014109019.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013964494.1 Canis lupus familiaris HECT domain containing E3 ubiquitin protein ligase 2 (HECTD2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe43981
Clone ID Related Accession (Same CDS sequence)	XM_014109019.1 , XM_014109019.2
Accession Version	XM_014109019.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	probable E3 ubiquitin-protein ligase HECTD2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGTG TGGCCTGGCC GTCCAGCCCG GTGGCGGAGA AGGTGGAGCC CACGACGTCT 
CACTTCCCCC CGCCCCCAGA TCCTTCTTCC GGTTTGGACA GAGGATCCAA AGGTCAAACT 
TCCACTTTTA GCAGTTTTAT TTCAACTGTT AACCAGAAGA AAGAAGCTGC TGAAAACAGA 
AGTTCACCTA CACATCTCAT TTTCCCTAAC ATCAAGAATG TGAGAGACCT ACCATCAATT 
TGCCTTGATG TTAGACAAAA ACAGCGCATT TCTGTAGACC CATTATCATC TGAAATAAAG 
GCCCCAGTTC TTCCAGAACC TGTCCTTCCT CGCCAGCCCA AAACTATGAA AGACTTTCAG 
GAAGATGTAG AAAAAGCTAA GTCAACAGGA GATTGGAAAG CAGTACATGA TTTTTATCTA 
ACAACATTTG ATTCTTTCCC AGAACTAAAT GCTGCATTTA AGAAAGATGC CACCGCCTCA 
TTTAATACCA TTGAAGATTC TGGGATTAAT GCTAAATTTG TGAATGCTGT GTATGATGCC 
TTACTTAATA CTCCTCAAGA CATTCAGAAG ACAGTATTAA AGGGAATCAT TAACAGCTTA 
TTACGAGAAT GGAAAGGTCC ACGAACAAAA GATGATCTTA GAGCATATTT TATACTTTTA 
CAGAATCCTC AATTTAATAA TACATCTACG TATGTCATCT ATGCTCACTT GCTACGGCAG 
ATAGCTACCT TAGTGGAAGC TGATCATCAT TTCCTAGTTC ACTGGTTTAA AAAAATATCC 
CAGAAGAGGT TCAAACAATT GGTAGAGAGA TTGCTGCAGT TTATTTCTTT GCGCCTGTTT 
CCTGCAAAGC CTGAAGAATT TCCACCTATC ACAAAGTGTT CCTGGTGGAT CCCATCAGCA 
TCTAAAGTGT TGGCTTTACT TAATACTGCA AACAGTTTGA TTCATCCTTC CCTTATTCCT 
TATACTGATT TCTATAATTC TACATTGGAT CACATTGATC TTATGGAAGA ATACCACACC 
TGGCAGAGCT TTGGAAACTC TCACAGGTTT TCCTTCTGTC AGTACCCATT CGTTATTTCT 
ATAGCTGCAA AAAAAATCAT TATTCAAAGA GACTCAGAAC AACAGATGAT AAGCATCGCA 
AGGCAAAGTC TGGTGGATAA AGTATCTCGA AGACAGAGAC CTGATATGAA CATGTTATTT 
CTAAATATGA AAGTAAGACG GACACATTTG GTTAGTGACT CGCTTGATGA GTTAACCCGG 
AAAAGAGCAG ACTTAAAAAA GAAGTTGAAA GTTACATTTG TAGGGGAAGC TGGTTTGGAC 
ATGGGTGGCT TGACTAAAGA ATGGTTCCTT CTTCTAATTC GCCAAATTTT TCATCCAGAT 
TATGGCATGT TTACATATCA CAAGGATTCA CACTGCCATT GGTTTAGCAG TTTTAAATGT 
GATAACTATT CTGAATTCCG ATTGGTTGGA ATTCTTATGG GACTAGCTGT GTATAACAGC 
ATCACCTTGG ATATTCGTTT CCCTCCCTGC TGTTACAAGA AATTATTGAG CCCTCCCATC 
ATTCCTAGTG ATCAAAATAT ACCAGTAGGC ATCTGCAGTG TGACCACTGA CGACTTATGT 
CAAATTATGC CTGAGTTGGC CCATGGGTTA AGTGAACTCT TGTCGTATGA AGGCAATGTT 
GAAGAAGATT TCTATTCAAC ATTCCAGGTT TTTCAAGAAG AATTTGGAAT AATTAAGTCC 
TACAATTTAA AGCCAGGTGG TGATAAAATT CCAGTTACCA ATCAAAATAG GAAAGAATAT 
GTACAGCTTT ATATTGACTT TCTTCTCAAC AAATCCATCT ATAAGCAGTT TGCTGCATTT 
TATTATGGAT TTCATAGTGT GTGTGCTTCA AATGCCCTGA TGCTGCTTCG TCCAGAAGAG 
GTTGAAATTC TGGTATGTGG AAGCCCTGAA CTGGATATGT ATGCTCTACA AAGAAGTACT 
CAGTATGATG GCTATGCCAA AACAGACTTA ACTATAAGAT ATTTTTGGGA TGTTGTGCTT 
GGATTTCCTC TTGATCTTCA AAAGAAGTTG CTACATTTTA CTACAGGAAG TGACAGAGTA 
CCGGTAGGAG GAATGGCTGA CTTGAACTTT AAAATCTCAA AGAATGAAAC TTCTACTAAC 
TGGTTGCCTG TGGCCCACAC CTGCTTCAAT CAACTTTGCC TTCCCCCCTA CAAGAGCAAA 
AAGGACCTAA AACAGAAATT GATCATTGGA ATTTCAAATT CAGAAGGTTT TGGACTTGAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013964494.1
CDS	56..2338
Translation	MESVAWPSSPVAEKVEPTTSHFPPPPDPSSGLDRGSKGQTSTFSSFISTVNQKKEAAENRSSPTHLIFPNIKNVRDLPSICLDVRQKQRISVDPLSSEIKAPVLPEPVLPRQPKTMKDFQEDVEKAKSTGDWKAVHDFYLTTFDSFPELNAAFKKDATASFNTIEDSGINAKFVNAVYDALLNTPQDIQKTVLKGIINSLLREWKGPRTKDDLRAYFILLQNPQFNNTSTYVIYAHLLRQIATLVEADHHFLVHWFKKISQKRFKQLVERLLQFISLRLFPAKPEEFPPITKCSWWIPSASKVLALLNTANSLIHPSLIPYTDFYNSTLDHIDLMEEYHTWQSFGNSHRFSFCQYPFVISIAAKKIIIQRDSEQQMISIARQSLVDKVSRRQRPDMNMLFLNMKVRRTHLVSDSLDELTRKRADLKKKLKVTFVGEAGLDMGGLTKEWFLLLIRQIFHPDYGMFTYHKDSHCHWFSSFKCDNYSEFRLVGILMGLAVYNSITLDIRFPPCCYKKLLSPPIIPSDQNIPVGICSVTTDDLCQIMPELAHGLSELLSYEGNVEEDFYSTFQVFQEEFGIIKSYNLKPGGDKIPVTNQNRKEYVQLYIDFLLNKSIYKQFAAFYYGFHSVCASNALMLLRPEEVEILVCGSPELDMYALQRSTQYDGYAKTDLTIRYFWDVVLGFPLDLQKKLLHFTTGSDRVPVGGMADLNFKISKNETSTNWLPVAHTCFNQLCLPPYKSKKDLKQKLIIGISNSEGFGLE

Target ORF information:

RefSeq Version	XM_014109019.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris HECT domain containing E3 ubiquitin protein ligase 2 (HECTD2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109019.1

ORF Insert Sequence:

ATGGAAAGTG TGGCCTGGCC GTCCAGCCCG GTGGCGGAGA AGGTGGAGCC CACGACGTCT 
CACTTCCCCC CGCCCCCAGA TCCTTCTTCC GGTTTGGACA GAGGATCCAA AGGTCAAACT 
TCCACTTTTA GCAGTTTTAT TTCAACTGTT AACCAGAAGA AAGAAGCTGC TGAAAACAGA 
AGTTCACCTA CACATCTCAT TTTCCCTAAC ATCAAGAATG TGAGAGACCT ACCATCAATT 
TGCCTTGATG TTAGACAAAA ACAGCGCATT TCTGTAGACC CATTATCATC TGAAATAAAG 
GCCCCAGTTC TTCCAGAACC TGTCCTTCCT CGCCAGCCCA AAACTATGAA AGACTTTCAG 
GAAGATGTAG AAAAAGCTAA GTCAACAGGA GATTGGAAAG CAGTACATGA TTTTTATCTA 
ACAACATTTG ATTCTTTCCC AGAACTAAAT GCTGCATTTA AGAAAGATGC CACCGCCTCA 
TTTAATACCA TTGAAGATTC TGGGATTAAT GCTAAATTTG TGAATGCTGT GTATGATGCC 
TTACTTAATA CTCCTCAAGA CATTCAGAAG ACAGTATTAA AGGGAATCAT TAACAGCTTA 
TTACGAGAAT GGAAAGGTCC ACGAACAAAA GATGATCTTA GAGCATATTT TATACTTTTA 
CAGAATCCTC AATTTAATAA TACATCTACG TATGTCATCT ATGCTCACTT GCTACGGCAG 
ATAGCTACCT TAGTGGAAGC TGATCATCAT TTCCTAGTTC ACTGGTTTAA AAAAATATCC 
CAGAAGAGGT TCAAACAATT GGTAGAGAGA TTGCTGCAGT TTATTTCTTT GCGCCTGTTT 
CCTGCAAAGC CTGAAGAATT TCCACCTATC ACAAAGTGTT CCTGGTGGAT CCCATCAGCA 
TCTAAAGTGT TGGCTTTACT TAATACTGCA AACAGTTTGA TTCATCCTTC CCTTATTCCT 
TATACTGATT TCTATAATTC TACATTGGAT CACATTGATC TTATGGAAGA ATACCACACC 
TGGCAGAGCT TTGGAAACTC TCACAGGTTT TCCTTCTGTC AGTACCCATT CGTTATTTCT 
ATAGCTGCAA AAAAAATCAT TATTCAAAGA GACTCAGAAC AACAGATGAT AAGCATCGCA 
AGGCAAAGTC TGGTGGATAA AGTATCTCGA AGACAGAGAC CTGATATGAA CATGTTATTT 
CTAAATATGA AAGTAAGACG GACACATTTG GTTAGTGACT CGCTTGATGA GTTAACCCGG 
AAAAGAGCAG ACTTAAAAAA GAAGTTGAAA GTTACATTTG TAGGGGAAGC TGGTTTGGAC 
ATGGGTGGCT TGACTAAAGA ATGGTTCCTT CTTCTAATTC GCCAAATTTT TCATCCAGAT 
TATGGCATGT TTACATATCA CAAGGATTCA CACTGCCATT GGTTTAGCAG TTTTAAATGT 
GATAACTATT CTGAATTCCG ATTGGTTGGA ATTCTTATGG GACTAGCTGT GTATAACAGC 
ATCACCTTGG ATATTCGTTT CCCTCCCTGC TGTTACAAGA AATTATTGAG CCCTCCCATC 
ATTCCTAGTG ATCAAAATAT ACCAGTAGGC ATCTGCAGTG TGACCACTGA CGACTTATGT 
CAAATTATGC CTGAGTTGGC CCATGGGTTA AGTGAACTCT TGTCGTATGA AGGCAATGTT 
GAAGAAGATT TCTATTCAAC ATTCCAGGTT TTTCAAGAAG AATTTGGAAT AATTAAGTCC 
TACAATTTAA AGCCAGGTGG TGATAAAATT CCAGTTACCA ATCAAAATAG GAAAGAATAT 
GTACAGCTTT ATATTGACTT TCTTCTCAAC AAATCCATCT ATAAGCAGTT TGCTGCATTT 
TATTATGGAT TTCATAGTGT GTGTGCTTCA AATGCCCTGA TGCTGCTTCG TCCAGAAGAG 
GTTGAAATTC TGGTATGTGG AAGCCCTGAA CTGGATATGT ATGCTCTACA AAGAAGTACT 
CAGTATGATG GCTATGCCAA AACAGACTTA ACTATAAGAT ATTTTTGGGA TGTTGTGCTT 
GGATTTCCTC TTGATCTTCA AAAGAAGTTG CTACATTTTA CTACAGGAAG TGACAGAGTA 
CCGGTAGGAG GAATGGCTGA CTTGAACTTT AAAATCTCAA AGAATGAAAC TTCTACTAAC 
TGGTTGCCTG TGGCCCACAC CTGCTTCAAT CAACTTTGCC TTCCCCCCTA CAAGAGCAAA 
AAGGACCTAA AACAGAAATT GATCATTGGA ATTTCAAATT CAGAAGGTTT TGGACTTGAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECTD2 ( XM_014109019.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013964494.1 Canis lupus familiaris HECT domain E3 ubiquitin protein ligase 2 (HECTD2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe43981
Clone ID Related Accession (Same CDS sequence)	XM_014109019.1 , XM_014109019.2
Accession Version	XM_014109019.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	probable E3 ubiquitin-protein ligase HECTD2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006610.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109019.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGTG TGGCCTGGCC GTCCAGCCCG GTGGCGGAGA AGGTGGAGCC CACGACGTCT 
CACTTCCCCC CGCCCCCAGA TCCTTCTTCC GGTTTGGACA GAGGATCCAA AGGTCAAACT 
TCCACTTTTA GCAGTTTTAT TTCAACTGTT AACCAGAAGA AAGAAGCTGC TGAAAACAGA 
AGTTCACCTA CACATCTCAT TTTCCCTAAC ATCAAGAATG TGAGAGACCT ACCATCAATT 
TGCCTTGATG TTAGACAAAA ACAGCGCATT TCTGTAGACC CATTATCATC TGAAATAAAG 
GCCCCAGTTC TTCCAGAACC TGTCCTTCCT CGCCAGCCCA AAACTATGAA AGACTTTCAG 
GAAGATGTAG AAAAAGCTAA GTCAACAGGA GATTGGAAAG CAGTACATGA TTTTTATCTA 
ACAACATTTG ATTCTTTCCC AGAACTAAAT GCTGCATTTA AGAAAGATGC CACCGCCTCA 
TTTAATACCA TTGAAGATTC TGGGATTAAT GCTAAATTTG TGAATGCTGT GTATGATGCC 
TTACTTAATA CTCCTCAAGA CATTCAGAAG ACAGTATTAA AGGGAATCAT TAACAGCTTA 
TTACGAGAAT GGAAAGGTCC ACGAACAAAA GATGATCTTA GAGCATATTT TATACTTTTA 
CAGAATCCTC AATTTAATAA TACATCTACG TATGTCATCT ATGCTCACTT GCTACGGCAG 
ATAGCTACCT TAGTGGAAGC TGATCATCAT TTCCTAGTTC ACTGGTTTAA AAAAATATCC 
CAGAAGAGGT TCAAACAATT GGTAGAGAGA TTGCTGCAGT TTATTTCTTT GCGCCTGTTT 
CCTGCAAAGC CTGAAGAATT TCCACCTATC ACAAAGTGTT CCTGGTGGAT CCCATCAGCA 
TCTAAAGTGT TGGCTTTACT TAATACTGCA AACAGTTTGA TTCATCCTTC CCTTATTCCT 
TATACTGATT TCTATAATTC TACATTGGAT CACATTGATC TTATGGAAGA ATACCACACC 
TGGCAGAGCT TTGGAAACTC TCACAGGTTT TCCTTCTGTC AGTACCCATT CGTTATTTCT 
ATAGCTGCAA AAAAAATCAT TATTCAAAGA GACTCAGAAC AACAGATGAT AAGCATCGCA 
AGGCAAAGTC TGGTGGATAA AGTATCTCGA AGACAGAGAC CTGATATGAA CATGTTATTT 
CTAAATATGA AAGTAAGACG GACACATTTG GTTAGTGACT CGCTTGATGA GTTAACCCGG 
AAAAGAGCAG ACTTAAAAAA GAAGTTGAAA GTTACATTTG TAGGGGAAGC TGGTTTGGAC 
ATGGGTGGCT TGACTAAAGA ATGGTTCCTT CTTCTAATTC GCCAAATTTT TCATCCAGAT 
TATGGCATGT TTACATATCA CAAGGATTCA CACTGCCATT GGTTTAGCAG TTTTAAATGT 
GATAACTATT CTGAATTCCG ATTGGTTGGA ATTCTTATGG GACTAGCTGT GTATAACAGC 
ATCACCTTGG ATATTCGTTT CCCTCCCTGC TGTTACAAGA AATTATTGAG CCCTCCCATC 
ATTCCTAGTG ATCAAAATAT ACCAGTAGGC ATCTGCAGTG TGACCACTGA CGACTTATGT 
CAAATTATGC CTGAGTTGGC CCATGGGTTA AGTGAACTCT TGTCGTATGA AGGCAATGTT 
GAAGAAGATT TCTATTCAAC ATTCCAGGTT TTTCAAGAAG AATTTGGAAT AATTAAGTCC 
TACAATTTAA AGCCAGGTGG TGATAAAATT CCAGTTACCA ATCAAAATAG GAAAGAATAT 
GTACAGCTTT ATATTGACTT TCTTCTCAAC AAATCCATCT ATAAGCAGTT TGCTGCATTT 
TATTATGGAT TTCATAGTGT GTGTGCTTCA AATGCCCTGA TGCTGCTTCG TCCAGAAGAG 
GTTGAAATTC TGGTATGTGG AAGCCCTGAA CTGGATATGT ATGCTCTACA AAGAAGTACT 
CAGTATGATG GCTATGCCAA AACAGACTTA ACTATAAGAT ATTTTTGGGA TGTTGTGCTT 
GGATTTCCTC TTGATCTTCA AAAGAAGTTG CTACATTTTA CTACAGGAAG TGACAGAGTA 
CCGGTAGGAG GAATGGCTGA CTTGAACTTT AAAATCTCAA AGAATGAAAC TTCTACTAAC 
TGGTTGCCTG TGGCCCACAC CTGCTTCAAT CAACTTTGCC TTCCCCCCTA CAAGAGCAAA 
AAGGACCTAA AACAGAAATT GATCATTGGA ATTTCAAATT CAGAAGGTTT TGGACTTGAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013964494.1
CDS	16..2298
Translation	MESVAWPSSPVAEKVEPTTSHFPPPPDPSSGLDRGSKGQTSTFSSFISTVNQKKEAAENRSSPTHLIFPNIKNVRDLPSICLDVRQKQRISVDPLSSEIKAPVLPEPVLPRQPKTMKDFQEDVEKAKSTGDWKAVHDFYLTTFDSFPELNAAFKKDATASFNTIEDSGINAKFVNAVYDALLNTPQDIQKTVLKGIINSLLREWKGPRTKDDLRAYFILLQNPQFNNTSTYVIYAHLLRQIATLVEADHHFLVHWFKKISQKRFKQLVERLLQFISLRLFPAKPEEFPPITKCSWWIPSASKVLALLNTANSLIHPSLIPYTDFYNSTLDHIDLMEEYHTWQSFGNSHRFSFCQYPFVISIAAKKIIIQRDSEQQMISIARQSLVDKVSRRQRPDMNMLFLNMKVRRTHLVSDSLDELTRKRADLKKKLKVTFVGEAGLDMGGLTKEWFLLLIRQIFHPDYGMFTYHKDSHCHWFSSFKCDNYSEFRLVGILMGLAVYNSITLDIRFPPCCYKKLLSPPIIPSDQNIPVGICSVTTDDLCQIMPELAHGLSELLSYEGNVEEDFYSTFQVFQEEFGIIKSYNLKPGGDKIPVTNQNRKEYVQLYIDFLLNKSIYKQFAAFYYGFHSVCASNALMLLRPEEVEILVCGSPELDMYALQRSTQYDGYAKTDLTIRYFWDVVLGFPLDLQKKLLHFTTGSDRVPVGGMADLNFKISKNETSTNWLPVAHTCFNQLCLPPYKSKKDLKQKLIIGISNSEGFGLE

Target ORF information:

RefSeq Version	XM_014109019.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris HECT domain E3 ubiquitin protein ligase 2 (HECTD2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109019.2

ORF Insert Sequence:

ATGGAAAGTG TGGCCTGGCC GTCCAGCCCG GTGGCGGAGA AGGTGGAGCC CACGACGTCT 
CACTTCCCCC CGCCCCCAGA TCCTTCTTCC GGTTTGGACA GAGGATCCAA AGGTCAAACT 
TCCACTTTTA GCAGTTTTAT TTCAACTGTT AACCAGAAGA AAGAAGCTGC TGAAAACAGA 
AGTTCACCTA CACATCTCAT TTTCCCTAAC ATCAAGAATG TGAGAGACCT ACCATCAATT 
TGCCTTGATG TTAGACAAAA ACAGCGCATT TCTGTAGACC CATTATCATC TGAAATAAAG 
GCCCCAGTTC TTCCAGAACC TGTCCTTCCT CGCCAGCCCA AAACTATGAA AGACTTTCAG 
GAAGATGTAG AAAAAGCTAA GTCAACAGGA GATTGGAAAG CAGTACATGA TTTTTATCTA 
ACAACATTTG ATTCTTTCCC AGAACTAAAT GCTGCATTTA AGAAAGATGC CACCGCCTCA 
TTTAATACCA TTGAAGATTC TGGGATTAAT GCTAAATTTG TGAATGCTGT GTATGATGCC 
TTACTTAATA CTCCTCAAGA CATTCAGAAG ACAGTATTAA AGGGAATCAT TAACAGCTTA 
TTACGAGAAT GGAAAGGTCC ACGAACAAAA GATGATCTTA GAGCATATTT TATACTTTTA 
CAGAATCCTC AATTTAATAA TACATCTACG TATGTCATCT ATGCTCACTT GCTACGGCAG 
ATAGCTACCT TAGTGGAAGC TGATCATCAT TTCCTAGTTC ACTGGTTTAA AAAAATATCC 
CAGAAGAGGT TCAAACAATT GGTAGAGAGA TTGCTGCAGT TTATTTCTTT GCGCCTGTTT 
CCTGCAAAGC CTGAAGAATT TCCACCTATC ACAAAGTGTT CCTGGTGGAT CCCATCAGCA 
TCTAAAGTGT TGGCTTTACT TAATACTGCA AACAGTTTGA TTCATCCTTC CCTTATTCCT 
TATACTGATT TCTATAATTC TACATTGGAT CACATTGATC TTATGGAAGA ATACCACACC 
TGGCAGAGCT TTGGAAACTC TCACAGGTTT TCCTTCTGTC AGTACCCATT CGTTATTTCT 
ATAGCTGCAA AAAAAATCAT TATTCAAAGA GACTCAGAAC AACAGATGAT AAGCATCGCA 
AGGCAAAGTC TGGTGGATAA AGTATCTCGA AGACAGAGAC CTGATATGAA CATGTTATTT 
CTAAATATGA AAGTAAGACG GACACATTTG GTTAGTGACT CGCTTGATGA GTTAACCCGG 
AAAAGAGCAG ACTTAAAAAA GAAGTTGAAA GTTACATTTG TAGGGGAAGC TGGTTTGGAC 
ATGGGTGGCT TGACTAAAGA ATGGTTCCTT CTTCTAATTC GCCAAATTTT TCATCCAGAT 
TATGGCATGT TTACATATCA CAAGGATTCA CACTGCCATT GGTTTAGCAG TTTTAAATGT 
GATAACTATT CTGAATTCCG ATTGGTTGGA ATTCTTATGG GACTAGCTGT GTATAACAGC 
ATCACCTTGG ATATTCGTTT CCCTCCCTGC TGTTACAAGA AATTATTGAG CCCTCCCATC 
ATTCCTAGTG ATCAAAATAT ACCAGTAGGC ATCTGCAGTG TGACCACTGA CGACTTATGT 
CAAATTATGC CTGAGTTGGC CCATGGGTTA AGTGAACTCT TGTCGTATGA AGGCAATGTT 
GAAGAAGATT TCTATTCAAC ATTCCAGGTT TTTCAAGAAG AATTTGGAAT AATTAAGTCC 
TACAATTTAA AGCCAGGTGG TGATAAAATT CCAGTTACCA ATCAAAATAG GAAAGAATAT 
GTACAGCTTT ATATTGACTT TCTTCTCAAC AAATCCATCT ATAAGCAGTT TGCTGCATTT 
TATTATGGAT TTCATAGTGT GTGTGCTTCA AATGCCCTGA TGCTGCTTCG TCCAGAAGAG 
GTTGAAATTC TGGTATGTGG AAGCCCTGAA CTGGATATGT ATGCTCTACA AAGAAGTACT 
CAGTATGATG GCTATGCCAA AACAGACTTA ACTATAAGAT ATTTTTGGGA TGTTGTGCTT 
GGATTTCCTC TTGATCTTCA AAAGAAGTTG CTACATTTTA CTACAGGAAG TGACAGAGTA 
CCGGTAGGAG GAATGGCTGA CTTGAACTTT AAAATCTCAA AGAATGAAAC TTCTACTAAC 
TGGTTGCCTG TGGCCCACAC CTGCTTCAAT CAACTTTGCC TTCCCCCCTA CAAGAGCAAA 
AAGGACCTAA AACAGAAATT GATCATTGGA ATTTCAAATT CAGAAGGTTT TGGACTTGAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECTD2 ( XM_014109020.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013964495.1 Canis lupus familiaris HECT domain containing E3 ubiquitin protein ligase 2 (HECTD2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe43982
Clone ID Related Accession (Same CDS sequence)	XM_014109020.1 , XM_014109020.2
Accession Version	XM_014109020.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1533bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	probable E3 ubiquitin-protein ligase HECTD2 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAAG AGGGCCCGTT GAGGGCGCCC GCCGGGCCGC CCGAGCTGGC GGCGGCCTCT 
TCGGAGGACA GGAAGGGAAG GGAGCCGGAG CGCGAGAAGC TGCCGCCCAT CGTGTCGGCG 
GGCGCCGGAG CGGCTGCGGC GGGTTTGGAC AGAGGATCCA AAGGTCAAAC TTCCACTTTT 
AGCAGTTTTA TTTCAACTGT TAACCAGAAG AAAGAAGCTG CTGAAAACAG AAGTTCACCT 
ACACATCTCA TTTTCCCTAA CATCAAGAAT GTGAGAGACC TACCATCAAT TTGCCTTGAT 
GTTAGACAAA AACAGCGCAT TTCTGTAGAC CCATTATCAT CTGAAATAAA GGCCCCAGTT 
CTTCCAGAAC CTGTCCTTCC TCGCCAGCCC AAAACTATGA AAGACTTTCA GGAAGATGTA 
GAAAAAGCTA AGTCAACAGG AGATTGGAAA GCAGTACATG ATTTTTATCT AACAACATTT 
GATTCTTTCC CAGAACTAAA TGCTGCATTT AAGAAAGATG CCACCGCCTC ATTTAATACC 
ATTGAAGATT CTGGGATTAA TGCTAAATTT GTGAATGCTG TGTATGATGC CTTACTTAAT 
ACTCCTCAAG ACATTCAGAA GACAGTATTA AAGGGAATCA TTAACAGCTT ATTACGAGAA 
TGGAAAGGTC CACGAACAAA AGATGATCTT AGAGCATATT TTATACTTTT ACAGAATCCT 
CAATTTAATA ATACATCTAC GTATGTCATC TATGCTCACT TGCTACGGCA GATAGCTACC 
TTAGTGGAAG CTGATCATCA TTTCCTAGTT CACTGGTTTA AAAAAATATC CCAGAAGAGG 
TTCAAACAAT TGGTAGAGAG ATTGCTGCAG TTTATTTCTT TGCGCCTGTT TCCTGCAAAG 
CCTGAAGAAT TTCCACCTAT CACAAAGTGT TCCTGGTGGA TCCCATCAGC ATCTAAAGTG 
TTGGCTTTAC TTAATACTGC AAACAGTTTG ATTCATCCTT CCCTTATTCC TTATACTGAT 
TTCTATAATT CTACATTGGA TCACATTGAT CTTATGGAAG AATACCACAC CTGGCAGAGC 
TTTGGAAACT CTCACAGGTT TTCCTTCTGT CAGTACCCAT TCGTTATTTC TATAGCTGCA 
AAAAAAATCA TTATTCAAAG AGACTCAGAA CAACAGATGA TAAGCATCGC AAGGCAAAGT 
CTGGTGGATA AAGTATCTCG AAGACAGAGA CCTGATATGA ACATGTTATT TCTAAATATG 
AAAGTAAGAC GGACACATTT GGTTAGTGAC TCGCTTGATG AGTTAACCCG GAAAAGAGCA 
GACTTAAAAA AGAAGTTGAA AGTTACATTT GTAGGGGAAG CTGGTTTGGA CATGGGTGGC 
TTGACTAAAG AATGGTTCCT TCTTCTAATT CGCCAAATTT TTCATCCAGA TTATGGCATG 
TTTACATATC ACAAGGATTC ACACTGCCAT TGGTTTAGCA GTTTTAAATG TGATAACTAT 
TCTGAATTCC GATTGGTTGG AATTTATGCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013964495.1
CDS	1..1533
Translation	MSEEGPLRAPAGPPELAAASSEDRKGREPEREKLPPIVSAGAGAAAAGLDRGSKGQTSTFSSFISTVNQKKEAAENRSSPTHLIFPNIKNVRDLPSICLDVRQKQRISVDPLSSEIKAPVLPEPVLPRQPKTMKDFQEDVEKAKSTGDWKAVHDFYLTTFDSFPELNAAFKKDATASFNTIEDSGINAKFVNAVYDALLNTPQDIQKTVLKGIINSLLREWKGPRTKDDLRAYFILLQNPQFNNTSTYVIYAHLLRQIATLVEADHHFLVHWFKKISQKRFKQLVERLLQFISLRLFPAKPEEFPPITKCSWWIPSASKVLALLNTANSLIHPSLIPYTDFYNSTLDHIDLMEEYHTWQSFGNSHRFSFCQYPFVISIAAKKIIIQRDSEQQMISIARQSLVDKVSRRQRPDMNMLFLNMKVRRTHLVSDSLDELTRKRADLKKKLKVTFVGEAGLDMGGLTKEWFLLLIRQIFHPDYGMFTYHKDSHCHWFSSFKCDNYSEFRLVGIYA

Target ORF information:

RefSeq Version	XM_014109020.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris HECT domain containing E3 ubiquitin protein ligase 2 (HECTD2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109020.1

ORF Insert Sequence:

ATGAGTGAAG AGGGCCCGTT GAGGGCGCCC GCCGGGCCGC CCGAGCTGGC GGCGGCCTCT 
TCGGAGGACA GGAAGGGAAG GGAGCCGGAG CGCGAGAAGC TGCCGCCCAT CGTGTCGGCG 
GGCGCCGGAG CGGCTGCGGC GGGTTTGGAC AGAGGATCCA AAGGTCAAAC TTCCACTTTT 
AGCAGTTTTA TTTCAACTGT TAACCAGAAG AAAGAAGCTG CTGAAAACAG AAGTTCACCT 
ACACATCTCA TTTTCCCTAA CATCAAGAAT GTGAGAGACC TACCATCAAT TTGCCTTGAT 
GTTAGACAAA AACAGCGCAT TTCTGTAGAC CCATTATCAT CTGAAATAAA GGCCCCAGTT 
CTTCCAGAAC CTGTCCTTCC TCGCCAGCCC AAAACTATGA AAGACTTTCA GGAAGATGTA 
GAAAAAGCTA AGTCAACAGG AGATTGGAAA GCAGTACATG ATTTTTATCT AACAACATTT 
GATTCTTTCC CAGAACTAAA TGCTGCATTT AAGAAAGATG CCACCGCCTC ATTTAATACC 
ATTGAAGATT CTGGGATTAA TGCTAAATTT GTGAATGCTG TGTATGATGC CTTACTTAAT 
ACTCCTCAAG ACATTCAGAA GACAGTATTA AAGGGAATCA TTAACAGCTT ATTACGAGAA 
TGGAAAGGTC CACGAACAAA AGATGATCTT AGAGCATATT TTATACTTTT ACAGAATCCT 
CAATTTAATA ATACATCTAC GTATGTCATC TATGCTCACT TGCTACGGCA GATAGCTACC 
TTAGTGGAAG CTGATCATCA TTTCCTAGTT CACTGGTTTA AAAAAATATC CCAGAAGAGG 
TTCAAACAAT TGGTAGAGAG ATTGCTGCAG TTTATTTCTT TGCGCCTGTT TCCTGCAAAG 
CCTGAAGAAT TTCCACCTAT CACAAAGTGT TCCTGGTGGA TCCCATCAGC ATCTAAAGTG 
TTGGCTTTAC TTAATACTGC AAACAGTTTG ATTCATCCTT CCCTTATTCC TTATACTGAT 
TTCTATAATT CTACATTGGA TCACATTGAT CTTATGGAAG AATACCACAC CTGGCAGAGC 
TTTGGAAACT CTCACAGGTT TTCCTTCTGT CAGTACCCAT TCGTTATTTC TATAGCTGCA 
AAAAAAATCA TTATTCAAAG AGACTCAGAA CAACAGATGA TAAGCATCGC AAGGCAAAGT 
CTGGTGGATA AAGTATCTCG AAGACAGAGA CCTGATATGA ACATGTTATT TCTAAATATG 
AAAGTAAGAC GGACACATTT GGTTAGTGAC TCGCTTGATG AGTTAACCCG GAAAAGAGCA 
GACTTAAAAA AGAAGTTGAA AGTTACATTT GTAGGGGAAG CTGGTTTGGA CATGGGTGGC 
TTGACTAAAG AATGGTTCCT TCTTCTAATT CGCCAAATTT TTCATCCAGA TTATGGCATG 
TTTACATATC ACAAGGATTC ACACTGCCAT TGGTTTAGCA GTTTTAAATG TGATAACTAT 
TCTGAATTCC GATTGGTTGG AATTTATGCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECTD2 ( XM_014109020.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013964495.1 Canis lupus familiaris HECT domain E3 ubiquitin protein ligase 2 (HECTD2), transcript variant X4, mRNA.

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CloneID	OCe43982
Clone ID Related Accession (Same CDS sequence)	XM_014109020.1 , XM_014109020.2
Accession Version	XM_014109020.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1533bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	probable E3 ubiquitin-protein ligase HECTD2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006610.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109020.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAAG AGGGCCCGTT GAGGGCGCCC GCCGGGCCGC CCGAGCTGGC GGCGGCCTCT 
TCGGAGGACA GGAAGGGAAG GGAGCCGGAG CGCGAGAAGC TGCCGCCCAT CGTGTCGGCG 
GGCGCCGGAG CGGCTGCGGC GGGTTTGGAC AGAGGATCCA AAGGTCAAAC TTCCACTTTT 
AGCAGTTTTA TTTCAACTGT TAACCAGAAG AAAGAAGCTG CTGAAAACAG AAGTTCACCT 
ACACATCTCA TTTTCCCTAA CATCAAGAAT GTGAGAGACC TACCATCAAT TTGCCTTGAT 
GTTAGACAAA AACAGCGCAT TTCTGTAGAC CCATTATCAT CTGAAATAAA GGCCCCAGTT 
CTTCCAGAAC CTGTCCTTCC TCGCCAGCCC AAAACTATGA AAGACTTTCA GGAAGATGTA 
GAAAAAGCTA AGTCAACAGG AGATTGGAAA GCAGTACATG ATTTTTATCT AACAACATTT 
GATTCTTTCC CAGAACTAAA TGCTGCATTT AAGAAAGATG CCACCGCCTC ATTTAATACC 
ATTGAAGATT CTGGGATTAA TGCTAAATTT GTGAATGCTG TGTATGATGC CTTACTTAAT 
ACTCCTCAAG ACATTCAGAA GACAGTATTA AAGGGAATCA TTAACAGCTT ATTACGAGAA 
TGGAAAGGTC CACGAACAAA AGATGATCTT AGAGCATATT TTATACTTTT ACAGAATCCT 
CAATTTAATA ATACATCTAC GTATGTCATC TATGCTCACT TGCTACGGCA GATAGCTACC 
TTAGTGGAAG CTGATCATCA TTTCCTAGTT CACTGGTTTA AAAAAATATC CCAGAAGAGG 
TTCAAACAAT TGGTAGAGAG ATTGCTGCAG TTTATTTCTT TGCGCCTGTT TCCTGCAAAG 
CCTGAAGAAT TTCCACCTAT CACAAAGTGT TCCTGGTGGA TCCCATCAGC ATCTAAAGTG 
TTGGCTTTAC TTAATACTGC AAACAGTTTG ATTCATCCTT CCCTTATTCC TTATACTGAT 
TTCTATAATT CTACATTGGA TCACATTGAT CTTATGGAAG AATACCACAC CTGGCAGAGC 
TTTGGAAACT CTCACAGGTT TTCCTTCTGT CAGTACCCAT TCGTTATTTC TATAGCTGCA 
AAAAAAATCA TTATTCAAAG AGACTCAGAA CAACAGATGA TAAGCATCGC AAGGCAAAGT 
CTGGTGGATA AAGTATCTCG AAGACAGAGA CCTGATATGA ACATGTTATT TCTAAATATG 
AAAGTAAGAC GGACACATTT GGTTAGTGAC TCGCTTGATG AGTTAACCCG GAAAAGAGCA 
GACTTAAAAA AGAAGTTGAA AGTTACATTT GTAGGGGAAG CTGGTTTGGA CATGGGTGGC 
TTGACTAAAG AATGGTTCCT TCTTCTAATT CGCCAAATTT TTCATCCAGA TTATGGCATG 
TTTACATATC ACAAGGATTC ACACTGCCAT TGGTTTAGCA GTTTTAAATG TGATAACTAT 
TCTGAATTCC GATTGGTTGG AATTTATGCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013964495.1
CDS	1..1533
Translation	MSEEGPLRAPAGPPELAAASSEDRKGREPEREKLPPIVSAGAGAAAAGLDRGSKGQTSTFSSFISTVNQKKEAAENRSSPTHLIFPNIKNVRDLPSICLDVRQKQRISVDPLSSEIKAPVLPEPVLPRQPKTMKDFQEDVEKAKSTGDWKAVHDFYLTTFDSFPELNAAFKKDATASFNTIEDSGINAKFVNAVYDALLNTPQDIQKTVLKGIINSLLREWKGPRTKDDLRAYFILLQNPQFNNTSTYVIYAHLLRQIATLVEADHHFLVHWFKKISQKRFKQLVERLLQFISLRLFPAKPEEFPPITKCSWWIPSASKVLALLNTANSLIHPSLIPYTDFYNSTLDHIDLMEEYHTWQSFGNSHRFSFCQYPFVISIAAKKIIIQRDSEQQMISIARQSLVDKVSRRQRPDMNMLFLNMKVRRTHLVSDSLDELTRKRADLKKKLKVTFVGEAGLDMGGLTKEWFLLLIRQIFHPDYGMFTYHKDSHCHWFSSFKCDNYSEFRLVGIYA

Target ORF information:

RefSeq Version	XM_014109020.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris HECT domain E3 ubiquitin protein ligase 2 (HECTD2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109020.2

ORF Insert Sequence:

ATGAGTGAAG AGGGCCCGTT GAGGGCGCCC GCCGGGCCGC CCGAGCTGGC GGCGGCCTCT 
TCGGAGGACA GGAAGGGAAG GGAGCCGGAG CGCGAGAAGC TGCCGCCCAT CGTGTCGGCG 
GGCGCCGGAG CGGCTGCGGC GGGTTTGGAC AGAGGATCCA AAGGTCAAAC TTCCACTTTT 
AGCAGTTTTA TTTCAACTGT TAACCAGAAG AAAGAAGCTG CTGAAAACAG AAGTTCACCT 
ACACATCTCA TTTTCCCTAA CATCAAGAAT GTGAGAGACC TACCATCAAT TTGCCTTGAT 
GTTAGACAAA AACAGCGCAT TTCTGTAGAC CCATTATCAT CTGAAATAAA GGCCCCAGTT 
CTTCCAGAAC CTGTCCTTCC TCGCCAGCCC AAAACTATGA AAGACTTTCA GGAAGATGTA 
GAAAAAGCTA AGTCAACAGG AGATTGGAAA GCAGTACATG ATTTTTATCT AACAACATTT 
GATTCTTTCC CAGAACTAAA TGCTGCATTT AAGAAAGATG CCACCGCCTC ATTTAATACC 
ATTGAAGATT CTGGGATTAA TGCTAAATTT GTGAATGCTG TGTATGATGC CTTACTTAAT 
ACTCCTCAAG ACATTCAGAA GACAGTATTA AAGGGAATCA TTAACAGCTT ATTACGAGAA 
TGGAAAGGTC CACGAACAAA AGATGATCTT AGAGCATATT TTATACTTTT ACAGAATCCT 
CAATTTAATA ATACATCTAC GTATGTCATC TATGCTCACT TGCTACGGCA GATAGCTACC 
TTAGTGGAAG CTGATCATCA TTTCCTAGTT CACTGGTTTA AAAAAATATC CCAGAAGAGG 
TTCAAACAAT TGGTAGAGAG ATTGCTGCAG TTTATTTCTT TGCGCCTGTT TCCTGCAAAG 
CCTGAAGAAT TTCCACCTAT CACAAAGTGT TCCTGGTGGA TCCCATCAGC ATCTAAAGTG 
TTGGCTTTAC TTAATACTGC AAACAGTTTG ATTCATCCTT CCCTTATTCC TTATACTGAT 
TTCTATAATT CTACATTGGA TCACATTGAT CTTATGGAAG AATACCACAC CTGGCAGAGC 
TTTGGAAACT CTCACAGGTT TTCCTTCTGT CAGTACCCAT TCGTTATTTC TATAGCTGCA 
AAAAAAATCA TTATTCAAAG AGACTCAGAA CAACAGATGA TAAGCATCGC AAGGCAAAGT 
CTGGTGGATA AAGTATCTCG AAGACAGAGA CCTGATATGA ACATGTTATT TCTAAATATG 
AAAGTAAGAC GGACACATTT GGTTAGTGAC TCGCTTGATG AGTTAACCCG GAAAAGAGCA 
GACTTAAAAA AGAAGTTGAA AGTTACATTT GTAGGGGAAG CTGGTTTGGA CATGGGTGGC 
TTGACTAAAG AATGGTTCCT TCTTCTAATT CGCCAAATTT TTCATCCAGA TTATGGCATG 
TTTACATATC ACAAGGATTC ACACTGCCAT TGGTTTAGCA GTTTTAAATG TGATAACTAT 
TCTGAATTCC GATTGGTTGG AATTTATGCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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HECTD2 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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