A2ML1 cDNA ORF clone, Canis lupus familiaris(dog)

The following A2ML1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the A2ML1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe282940 XM_022411340.1
Latest version!
Canis lupus familiaris alpha-2-macroglobulin like 1 (A2ML1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe282940 XM_022411341.1
Latest version!
Canis lupus familiaris alpha-2-macroglobulin like 1 (A2ML1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe282941 XM_022411342.1
Latest version!
Canis lupus familiaris alpha-2-macroglobulin like 1 (A2ML1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe43565 XM_014108319.1
Latest version!
Canis lupus familiaris alpha-2-macroglobulin-like 1 (A2ML1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe282940
    Clone ID Related Accession (Same CDS sequence) XM_022411340.1 , XM_022411341.1
    Accession Version XM_022411340.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product alpha-2-macroglobulin-like protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGTGGAAC TGCAGGATCC AAATAGCAAC AGGATTGCAC AGTGGCTGGA AGTGGTACCT 
    CAGCAAGGCA TTGCAGACCT ATCCTTCCAA CTGGCACCAG AGGCAATGCT GGGAACCTAT
    ATGGTAGCAG TGGCTGGGGG CATGGCCTTT GGCACCTTCA GTGTGGAAGA ATATGTGTTG
    CCTAAGTTTA AGGTGGATGT GGTGGAGCCC AAGCAGTTAT CAACAGTGGA AGAATCCTTC
    TTAGTCAAGA TTTGTTGTAG GTACACCTAC GGAAAGCCCA TGCTAGGGAC AGCACAGGTA
    TCAGTGTGTC AAAAGCCATA TAGCTACCAT TTTCCAGAGC CAGAACAGCA ACAGCTACCT
    GACAGATGCA CAAACCTTTC TGGACAGACT GACAAAGCAG GATGCTTCTC AGCTTCTGTG
    GACATGTCTA CCTTCAACCT CACTGGGTAT ATGTACAGCC ACAGCATCAA TATTGTGGCT
    ATTGTGGTGG AAGAAGGAAC AGGAGTAGAG GCCAATACTA CTCGGGATAT CTACATTTCT
    TCACAAATGG GATCAATAAC CTTTGAAGAC ACCAAAAATT TTTATTACCC CAATTTTCCC
    TTCAGTGGGA AGGTAAGAGT TAGGGGCCAT GATGGCTCCC TTCTCAAGAA TCATCCAGTA
    TTTTTGGTGA TTAATGGCAT AAATGGAACC ATCAACCAGA CCCTAACAAC TGACAATGAT
    GGCCTTGCTC CCTTCAAGCT GGATACAGTA AATTGGAATG GGAGAGATAT TTCTCTGGAG
    GCCAGATTCC AAATGGAAGA TTTGGTATAT AATCCAGGAG AGAGGCCTCA TTACTACCAA
    AATGATTATC TGCACCTGCA GGCCTTCTAC AACACAACTC AGAGCTTCCT TGGCATCCAC
    CCGCTAAGTG GACTCTTGGC ATGTAGCCAG CCCCAGGAAG TGCTGGTGGA CTATTACATT
    GATCCAGCTG ATGCAAACCC TGACCAGGAA ATCATCTTCT CCTACTATTT AATAGGGAAA
    GGGCACTTGG AGATGGAGGG ACAGAAACAC CTGAACTCTG AGAAGGAAGG AATGAAAGGC
    TCCTTCTCCC TCTCACTGAC CTTCAATTCC AGACTAGCCC CTGATCCTTC CCTAGTGGTC
    TATGCCATTT TTCCCAGTGG AGGCATTATA GCTGACAAAA TTCAGTTCTC AGTAGAGATG
    TGTTTTGACA ATCAGGTTTC CCTTGGCTTC TCACCTTCCA AGCAGCTTCC AGGAGCAGAT
    GTGGAACTAC AGCTACAGGC AGCTCCTGGA TCGCTGTGTG CAGTCCGGGC TGTGGATGAG
    AGTGTCTCAC TGCTTAGGCC GGAGAGAGAG CTAAGCAACA ACTCTGTCTA TAGGATGTTC
    TCATTCTGGT ATGGCCACTA CCCCTATCAG GTGGCTGAAT ATGATGAGTG TCCAGTGTCT
    GGCCCCTGGT ATGCTCCTCA GCCCCTCTCA GCTATGTGGA GGCCTTGGTT CTCTGAAGGC
    ATGGACCTTT TCCATCTTTT CCAGGGCATG GGCCTGAAAA TACTGTCCAA TGCCAAAATT
    AAGAAGCCAG TAGATTGCAT TCACCAGCCT CTGGAATACA GCTTTTCAAT GGGTGAAGCA
    AATTATAATA TTGGTACAGT GAATTCTGAG TCATCATCTT CTTCATCACT TCAGTCAGAG
    GACTCTCAGG TCCGCCAGTA CTTCCCGGAG ACCTGGCTCT GGGATCTGTT TCCTATTGGT
    GACTCAGGGA AGGAGGCTGT CCATCTTACA GTTCCTGATA CCATCACTGA GTGGAAGGCC
    ATGACTTTTT GTACCTCCCA GTCCAGCGGC TTTGGTCTTT CACCTACTGT TGGACTGACT
    GCTTTCAAGC CATTCTTTGT TGACCTGACT CTCCCTTACT CAGTAGTTCG TGGGGAATCC
    TTTCGCCTTA CTGCCACCAT CTTCAATTAT CTAAAGGACT GCATCAGGGT TCAGACTAAT
    CTGGCTACAT CAGATAAATA CCAGGTGGAA TCGTGGACAG ATTCTCAGGG ATCCAGCTGT
    CTCTGTGCTG ATGAAGCAAA AACGTATCAC TGGAATATCA CAGCTATCAA ATTGGGTCAT
    GTAAACTTTA CTATCACTAC TAAGATTCTG GACAGCAGTG AACTCTGTAG GGGGGAAAAG
    GGGTTCATTC CAGCAAAGGG CCAGAGTGAC ACACTTATCA AACCAGTTCT GGTCAAGCCT
    GAGGGAGTCC TTGTGGAGAA GACATACAGC TCATTGCTAT GCCCAAAAGG ACAAGTGGCT
    TCAGAATCTA TCTCCCTGGA GCTCCCTGTG GATGTTGTTC CTGATTCAAC CAAGGCTTAT
    GTTACAGTTC TGGGAGACAT TATGGGCACG GCCCTACAGA ACCTAGACAA TCTGGTGCAG
    ATGCCAAGGG GCTGTGGGGA GCAGAACATG GTCTTGTTTG CTCCCATAAT CTACGTCTTG
    CAGTATCTGG AGAGGGCAAG GCTGCTGACT GAAGAAATCA AGTCTCAGGC AGTGGGTTTC
    CTGAAAATAG GGTACCAGAA GGAGCTAATG TACAAACACA GCAACGGCTC ATACAGTGCC
    TTTGGGGAGC AGGATGGAGA TGGAAACACA TGGCTGACAG CGTTTGTCAC CAAATGCTTT
    GGCCAAGCTC AAGAATTTAT CTTCATTGAT GACAAGAATA TCCAGGATGC TCTCAAGTGG
    ATGGCGGGAA ACCAGCTCCC CAACGGTTGC TATGCTAATG TGGGAAACCT CATTCACACA
    GCTATGAAGG GCGGTGTTGA AGATGAGATT TCCCTGACTG CATACATCAC AGCTGCATTG
    CTGGAAATGG GAATGACCGC ACATGACCCA ATGGTGAGTC AGGGTCTGAA GTGCCTCCGA
    GACTCTGTTT CCTCTACTAC CAACCTCTAC ACACAGGGCC TCCTTGCTTA TACTTTCTCT
    CTGGCTGGGG AGATGGACAT CAGAAACGTT CTTCTTGAAA AGTTAGACCA GCAGGCTATC
    ATCTCAGGAG GGTCTATTCA CTGGAGCCAA AAGCCTATTC CGTCATTGGA TGACAGACCT
    TGGTCTCAGC CTGAGGCTGT AGATGTGGAA CTTACAGCAT ATATATTACT GGCCCAACTT
    AGCAAAGCCA GCCTGACTCA AAAGGAGATA GCCAAGGCCA CTGCCATAGT GGCTTGGTTA
    ACCAAGCAAC GTAATGCATA TGGGGGCTTC TCTTCTACTC AGGATACTGT AGTTGCTCTC
    CAAGCTCTTG CCAAATATGC CACTGTAGCC TATGTAACAT CTGAAAAAGT CAACCTGGCT
    GTAAAATCCA ATAAGAATTT CCAGCACACT TTCAGCATTC AGGCTGCCAA CCGGTTGGTG
    CTTCAGCAGG AAACTCTGCC CAGTATCCCT GGAGTGTACA CCTTGGAAGC CTCAGGCCAG
    GGCTGTGTCT ATGTGCAGAT GGTGCTGATA TACCATATTC CCCCTCCTAA GTTTGTGGAC
    ACCTTTAGTC TTAGTGTGGA AATGGGAAAA GCTAGATGTG AGCAAGCTAC TTCACCTCGA
    TCCTTGATGC TCACCATACA CACCAGTTAC GTGGGGAGTC GCAGTTCTTC CAATATGGCC
    ATTGTAGAAG TGAAGATGCT TTCTGGGTTC AGCCCCAAGG AGGGTACCAA TCAGTTACTT
    CTCCAACAAC CTCTGGTGAA GAAGGTGGAA TCTGGAACTG ATATCCTGAA CATTTATTTG
    GAAGAGCTGA GCAAGAAGAC TCAGACCTAT TCCTTTACCA TCAGCCAAAG TGTGTTGGTC
    ACTAACCTGA AACCAGCAAC CATCACAGTC TATGACTACT ACCTCCCAGA TGAACGGGCA
    ACAATTCAGT ACTCTGACCC CTGTGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022267048.1
    CDS129..3998
    Translation

    Target ORF information:

    RefSeq Version XM_022411340.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris alpha-2-macroglobulin like 1 (A2ML1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022411340.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGTGGAAC TGCAGGATCC AAATAGCAAC AGGATTGCAC AGTGGCTGGA AGTGGTACCT 
    CAGCAAGGCA TTGCAGACCT ATCCTTCCAA CTGGCACCAG AGGCAATGCT GGGAACCTAT
    ATGGTAGCAG TGGCTGGGGG CATGGCCTTT GGCACCTTCA GTGTGGAAGA ATATGTGTTG
    CCTAAGTTTA AGGTGGATGT GGTGGAGCCC AAGCAGTTAT CAACAGTGGA AGAATCCTTC
    TTAGTCAAGA TTTGTTGTAG GTACACCTAC GGAAAGCCCA TGCTAGGGAC AGCACAGGTA
    TCAGTGTGTC AAAAGCCATA TAGCTACCAT TTTCCAGAGC CAGAACAGCA ACAGCTACCT
    GACAGATGCA CAAACCTTTC TGGACAGACT GACAAAGCAG GATGCTTCTC AGCTTCTGTG
    GACATGTCTA CCTTCAACCT CACTGGGTAT ATGTACAGCC ACAGCATCAA TATTGTGGCT
    ATTGTGGTGG AAGAAGGAAC AGGAGTAGAG GCCAATACTA CTCGGGATAT CTACATTTCT
    TCACAAATGG GATCAATAAC CTTTGAAGAC ACCAAAAATT TTTATTACCC CAATTTTCCC
    TTCAGTGGGA AGGTAAGAGT TAGGGGCCAT GATGGCTCCC TTCTCAAGAA TCATCCAGTA
    TTTTTGGTGA TTAATGGCAT AAATGGAACC ATCAACCAGA CCCTAACAAC TGACAATGAT
    GGCCTTGCTC CCTTCAAGCT GGATACAGTA AATTGGAATG GGAGAGATAT TTCTCTGGAG
    GCCAGATTCC AAATGGAAGA TTTGGTATAT AATCCAGGAG AGAGGCCTCA TTACTACCAA
    AATGATTATC TGCACCTGCA GGCCTTCTAC AACACAACTC AGAGCTTCCT TGGCATCCAC
    CCGCTAAGTG GACTCTTGGC ATGTAGCCAG CCCCAGGAAG TGCTGGTGGA CTATTACATT
    GATCCAGCTG ATGCAAACCC TGACCAGGAA ATCATCTTCT CCTACTATTT AATAGGGAAA
    GGGCACTTGG AGATGGAGGG ACAGAAACAC CTGAACTCTG AGAAGGAAGG AATGAAAGGC
    TCCTTCTCCC TCTCACTGAC CTTCAATTCC AGACTAGCCC CTGATCCTTC CCTAGTGGTC
    TATGCCATTT TTCCCAGTGG AGGCATTATA GCTGACAAAA TTCAGTTCTC AGTAGAGATG
    TGTTTTGACA ATCAGGTTTC CCTTGGCTTC TCACCTTCCA AGCAGCTTCC AGGAGCAGAT
    GTGGAACTAC AGCTACAGGC AGCTCCTGGA TCGCTGTGTG CAGTCCGGGC TGTGGATGAG
    AGTGTCTCAC TGCTTAGGCC GGAGAGAGAG CTAAGCAACA ACTCTGTCTA TAGGATGTTC
    TCATTCTGGT ATGGCCACTA CCCCTATCAG GTGGCTGAAT ATGATGAGTG TCCAGTGTCT
    GGCCCCTGGT ATGCTCCTCA GCCCCTCTCA GCTATGTGGA GGCCTTGGTT CTCTGAAGGC
    ATGGACCTTT TCCATCTTTT CCAGGGCATG GGCCTGAAAA TACTGTCCAA TGCCAAAATT
    AAGAAGCCAG TAGATTGCAT TCACCAGCCT CTGGAATACA GCTTTTCAAT GGGTGAAGCA
    AATTATAATA TTGGTACAGT GAATTCTGAG TCATCATCTT CTTCATCACT TCAGTCAGAG
    GACTCTCAGG TCCGCCAGTA CTTCCCGGAG ACCTGGCTCT GGGATCTGTT TCCTATTGGT
    GACTCAGGGA AGGAGGCTGT CCATCTTACA GTTCCTGATA CCATCACTGA GTGGAAGGCC
    ATGACTTTTT GTACCTCCCA GTCCAGCGGC TTTGGTCTTT CACCTACTGT TGGACTGACT
    GCTTTCAAGC CATTCTTTGT TGACCTGACT CTCCCTTACT CAGTAGTTCG TGGGGAATCC
    TTTCGCCTTA CTGCCACCAT CTTCAATTAT CTAAAGGACT GCATCAGGGT TCAGACTAAT
    CTGGCTACAT CAGATAAATA CCAGGTGGAA TCGTGGACAG ATTCTCAGGG ATCCAGCTGT
    CTCTGTGCTG ATGAAGCAAA AACGTATCAC TGGAATATCA CAGCTATCAA ATTGGGTCAT
    GTAAACTTTA CTATCACTAC TAAGATTCTG GACAGCAGTG AACTCTGTAG GGGGGAAAAG
    GGGTTCATTC CAGCAAAGGG CCAGAGTGAC ACACTTATCA AACCAGTTCT GGTCAAGCCT
    GAGGGAGTCC TTGTGGAGAA GACATACAGC TCATTGCTAT GCCCAAAAGG ACAAGTGGCT
    TCAGAATCTA TCTCCCTGGA GCTCCCTGTG GATGTTGTTC CTGATTCAAC CAAGGCTTAT
    GTTACAGTTC TGGGAGACAT TATGGGCACG GCCCTACAGA ACCTAGACAA TCTGGTGCAG
    ATGCCAAGGG GCTGTGGGGA GCAGAACATG GTCTTGTTTG CTCCCATAAT CTACGTCTTG
    CAGTATCTGG AGAGGGCAAG GCTGCTGACT GAAGAAATCA AGTCTCAGGC AGTGGGTTTC
    CTGAAAATAG GGTACCAGAA GGAGCTAATG TACAAACACA GCAACGGCTC ATACAGTGCC
    TTTGGGGAGC AGGATGGAGA TGGAAACACA TGGCTGACAG CGTTTGTCAC CAAATGCTTT
    GGCCAAGCTC AAGAATTTAT CTTCATTGAT GACAAGAATA TCCAGGATGC TCTCAAGTGG
    ATGGCGGGAA ACCAGCTCCC CAACGGTTGC TATGCTAATG TGGGAAACCT CATTCACACA
    GCTATGAAGG GCGGTGTTGA AGATGAGATT TCCCTGACTG CATACATCAC AGCTGCATTG
    CTGGAAATGG GAATGACCGC ACATGACCCA ATGGTGAGTC AGGGTCTGAA GTGCCTCCGA
    GACTCTGTTT CCTCTACTAC CAACCTCTAC ACACAGGGCC TCCTTGCTTA TACTTTCTCT
    CTGGCTGGGG AGATGGACAT CAGAAACGTT CTTCTTGAAA AGTTAGACCA GCAGGCTATC
    ATCTCAGGAG GGTCTATTCA CTGGAGCCAA AAGCCTATTC CGTCATTGGA TGACAGACCT
    TGGTCTCAGC CTGAGGCTGT AGATGTGGAA CTTACAGCAT ATATATTACT GGCCCAACTT
    AGCAAAGCCA GCCTGACTCA AAAGGAGATA GCCAAGGCCA CTGCCATAGT GGCTTGGTTA
    ACCAAGCAAC GTAATGCATA TGGGGGCTTC TCTTCTACTC AGGATACTGT AGTTGCTCTC
    CAAGCTCTTG CCAAATATGC CACTGTAGCC TATGTAACAT CTGAAAAAGT CAACCTGGCT
    GTAAAATCCA ATAAGAATTT CCAGCACACT TTCAGCATTC AGGCTGCCAA CCGGTTGGTG
    CTTCAGCAGG AAACTCTGCC CAGTATCCCT GGAGTGTACA CCTTGGAAGC CTCAGGCCAG
    GGCTGTGTCT ATGTGCAGAT GGTGCTGATA TACCATATTC CCCCTCCTAA GTTTGTGGAC
    ACCTTTAGTC TTAGTGTGGA AATGGGAAAA GCTAGATGTG AGCAAGCTAC TTCACCTCGA
    TCCTTGATGC TCACCATACA CACCAGTTAC GTGGGGAGTC GCAGTTCTTC CAATATGGCC
    ATTGTAGAAG TGAAGATGCT TTCTGGGTTC AGCCCCAAGG AGGGTACCAA TCAGTTACTT
    CTCCAACAAC CTCTGGTGAA GAAGGTGGAA TCTGGAACTG ATATCCTGAA CATTTATTTG
    GAAGAGCTGA GCAAGAAGAC TCAGACCTAT TCCTTTACCA TCAGCCAAAG TGTGTTGGTC
    ACTAACCTGA AACCAGCAAC CATCACAGTC TATGACTACT ACCTCCCAGA TGAACGGGCA
    ACAATTCAGT ACTCTGACCC CTGTGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe282940
    Clone ID Related Accession (Same CDS sequence) XM_022411340.1 , XM_022411341.1
    Accession Version XM_022411341.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product alpha-2-macroglobulin-like protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGTGGAAC TGCAGGATCC AAATAGCAAC AGGATTGCAC AGTGGCTGGA AGTGGTACCT 
    CAGCAAGGCA TTGCAGACCT ATCCTTCCAA CTGGCACCAG AGGCAATGCT GGGAACCTAT
    ATGGTAGCAG TGGCTGGGGG CATGGCCTTT GGCACCTTCA GTGTGGAAGA ATATGTGTTG
    CCTAAGTTTA AGGTGGATGT GGTGGAGCCC AAGCAGTTAT CAACAGTGGA AGAATCCTTC
    TTAGTCAAGA TTTGTTGTAG GTACACCTAC GGAAAGCCCA TGCTAGGGAC AGCACAGGTA
    TCAGTGTGTC AAAAGCCATA TAGCTACCAT TTTCCAGAGC CAGAACAGCA ACAGCTACCT
    GACAGATGCA CAAACCTTTC TGGACAGACT GACAAAGCAG GATGCTTCTC AGCTTCTGTG
    GACATGTCTA CCTTCAACCT CACTGGGTAT ATGTACAGCC ACAGCATCAA TATTGTGGCT
    ATTGTGGTGG AAGAAGGAAC AGGAGTAGAG GCCAATACTA CTCGGGATAT CTACATTTCT
    TCACAAATGG GATCAATAAC CTTTGAAGAC ACCAAAAATT TTTATTACCC CAATTTTCCC
    TTCAGTGGGA AGGTAAGAGT TAGGGGCCAT GATGGCTCCC TTCTCAAGAA TCATCCAGTA
    TTTTTGGTGA TTAATGGCAT AAATGGAACC ATCAACCAGA CCCTAACAAC TGACAATGAT
    GGCCTTGCTC CCTTCAAGCT GGATACAGTA AATTGGAATG GGAGAGATAT TTCTCTGGAG
    GCCAGATTCC AAATGGAAGA TTTGGTATAT AATCCAGGAG AGAGGCCTCA TTACTACCAA
    AATGATTATC TGCACCTGCA GGCCTTCTAC AACACAACTC AGAGCTTCCT TGGCATCCAC
    CCGCTAAGTG GACTCTTGGC ATGTAGCCAG CCCCAGGAAG TGCTGGTGGA CTATTACATT
    GATCCAGCTG ATGCAAACCC TGACCAGGAA ATCATCTTCT CCTACTATTT AATAGGGAAA
    GGGCACTTGG AGATGGAGGG ACAGAAACAC CTGAACTCTG AGAAGGAAGG AATGAAAGGC
    TCCTTCTCCC TCTCACTGAC CTTCAATTCC AGACTAGCCC CTGATCCTTC CCTAGTGGTC
    TATGCCATTT TTCCCAGTGG AGGCATTATA GCTGACAAAA TTCAGTTCTC AGTAGAGATG
    TGTTTTGACA ATCAGGTTTC CCTTGGCTTC TCACCTTCCA AGCAGCTTCC AGGAGCAGAT
    GTGGAACTAC AGCTACAGGC AGCTCCTGGA TCGCTGTGTG CAGTCCGGGC TGTGGATGAG
    AGTGTCTCAC TGCTTAGGCC GGAGAGAGAG CTAAGCAACA ACTCTGTCTA TAGGATGTTC
    TCATTCTGGT ATGGCCACTA CCCCTATCAG GTGGCTGAAT ATGATGAGTG TCCAGTGTCT
    GGCCCCTGGT ATGCTCCTCA GCCCCTCTCA GCTATGTGGA GGCCTTGGTT CTCTGAAGGC
    ATGGACCTTT TCCATCTTTT CCAGGGCATG GGCCTGAAAA TACTGTCCAA TGCCAAAATT
    AAGAAGCCAG TAGATTGCAT TCACCAGCCT CTGGAATACA GCTTTTCAAT GGGTGAAGCA
    AATTATAATA TTGGTACAGT GAATTCTGAG TCATCATCTT CTTCATCACT TCAGTCAGAG
    GACTCTCAGG TCCGCCAGTA CTTCCCGGAG ACCTGGCTCT GGGATCTGTT TCCTATTGGT
    GACTCAGGGA AGGAGGCTGT CCATCTTACA GTTCCTGATA CCATCACTGA GTGGAAGGCC
    ATGACTTTTT GTACCTCCCA GTCCAGCGGC TTTGGTCTTT CACCTACTGT TGGACTGACT
    GCTTTCAAGC CATTCTTTGT TGACCTGACT CTCCCTTACT CAGTAGTTCG TGGGGAATCC
    TTTCGCCTTA CTGCCACCAT CTTCAATTAT CTAAAGGACT GCATCAGGGT TCAGACTAAT
    CTGGCTACAT CAGATAAATA CCAGGTGGAA TCGTGGACAG ATTCTCAGGG ATCCAGCTGT
    CTCTGTGCTG ATGAAGCAAA AACGTATCAC TGGAATATCA CAGCTATCAA ATTGGGTCAT
    GTAAACTTTA CTATCACTAC TAAGATTCTG GACAGCAGTG AACTCTGTAG GGGGGAAAAG
    GGGTTCATTC CAGCAAAGGG CCAGAGTGAC ACACTTATCA AACCAGTTCT GGTCAAGCCT
    GAGGGAGTCC TTGTGGAGAA GACATACAGC TCATTGCTAT GCCCAAAAGG ACAAGTGGCT
    TCAGAATCTA TCTCCCTGGA GCTCCCTGTG GATGTTGTTC CTGATTCAAC CAAGGCTTAT
    GTTACAGTTC TGGGAGACAT TATGGGCACG GCCCTACAGA ACCTAGACAA TCTGGTGCAG
    ATGCCAAGGG GCTGTGGGGA GCAGAACATG GTCTTGTTTG CTCCCATAAT CTACGTCTTG
    CAGTATCTGG AGAGGGCAAG GCTGCTGACT GAAGAAATCA AGTCTCAGGC AGTGGGTTTC
    CTGAAAATAG GGTACCAGAA GGAGCTAATG TACAAACACA GCAACGGCTC ATACAGTGCC
    TTTGGGGAGC AGGATGGAGA TGGAAACACA TGGCTGACAG CGTTTGTCAC CAAATGCTTT
    GGCCAAGCTC AAGAATTTAT CTTCATTGAT GACAAGAATA TCCAGGATGC TCTCAAGTGG
    ATGGCGGGAA ACCAGCTCCC CAACGGTTGC TATGCTAATG TGGGAAACCT CATTCACACA
    GCTATGAAGG GCGGTGTTGA AGATGAGATT TCCCTGACTG CATACATCAC AGCTGCATTG
    CTGGAAATGG GAATGACCGC ACATGACCCA ATGGTGAGTC AGGGTCTGAA GTGCCTCCGA
    GACTCTGTTT CCTCTACTAC CAACCTCTAC ACACAGGGCC TCCTTGCTTA TACTTTCTCT
    CTGGCTGGGG AGATGGACAT CAGAAACGTT CTTCTTGAAA AGTTAGACCA GCAGGCTATC
    ATCTCAGGAG GGTCTATTCA CTGGAGCCAA AAGCCTATTC CGTCATTGGA TGACAGACCT
    TGGTCTCAGC CTGAGGCTGT AGATGTGGAA CTTACAGCAT ATATATTACT GGCCCAACTT
    AGCAAAGCCA GCCTGACTCA AAAGGAGATA GCCAAGGCCA CTGCCATAGT GGCTTGGTTA
    ACCAAGCAAC GTAATGCATA TGGGGGCTTC TCTTCTACTC AGGATACTGT AGTTGCTCTC
    CAAGCTCTTG CCAAATATGC CACTGTAGCC TATGTAACAT CTGAAAAAGT CAACCTGGCT
    GTAAAATCCA ATAAGAATTT CCAGCACACT TTCAGCATTC AGGCTGCCAA CCGGTTGGTG
    CTTCAGCAGG AAACTCTGCC CAGTATCCCT GGAGTGTACA CCTTGGAAGC CTCAGGCCAG
    GGCTGTGTCT ATGTGCAGAT GGTGCTGATA TACCATATTC CCCCTCCTAA GTTTGTGGAC
    ACCTTTAGTC TTAGTGTGGA AATGGGAAAA GCTAGATGTG AGCAAGCTAC TTCACCTCGA
    TCCTTGATGC TCACCATACA CACCAGTTAC GTGGGGAGTC GCAGTTCTTC CAATATGGCC
    ATTGTAGAAG TGAAGATGCT TTCTGGGTTC AGCCCCAAGG AGGGTACCAA TCAGTTACTT
    CTCCAACAAC CTCTGGTGAA GAAGGTGGAA TCTGGAACTG ATATCCTGAA CATTTATTTG
    GAAGAGCTGA GCAAGAAGAC TCAGACCTAT TCCTTTACCA TCAGCCAAAG TGTGTTGGTC
    ACTAACCTGA AACCAGCAAC CATCACAGTC TATGACTACT ACCTCCCAGA TGAACGGGCA
    ACAATTCAGT ACTCTGACCC CTGTGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022267049.1
    CDS140..4009
    Translation

    Target ORF information:

    RefSeq Version XM_022411341.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris alpha-2-macroglobulin like 1 (A2ML1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022411341.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGTGGAAC TGCAGGATCC AAATAGCAAC AGGATTGCAC AGTGGCTGGA AGTGGTACCT 
    CAGCAAGGCA TTGCAGACCT ATCCTTCCAA CTGGCACCAG AGGCAATGCT GGGAACCTAT
    ATGGTAGCAG TGGCTGGGGG CATGGCCTTT GGCACCTTCA GTGTGGAAGA ATATGTGTTG
    CCTAAGTTTA AGGTGGATGT GGTGGAGCCC AAGCAGTTAT CAACAGTGGA AGAATCCTTC
    TTAGTCAAGA TTTGTTGTAG GTACACCTAC GGAAAGCCCA TGCTAGGGAC AGCACAGGTA
    TCAGTGTGTC AAAAGCCATA TAGCTACCAT TTTCCAGAGC CAGAACAGCA ACAGCTACCT
    GACAGATGCA CAAACCTTTC TGGACAGACT GACAAAGCAG GATGCTTCTC AGCTTCTGTG
    GACATGTCTA CCTTCAACCT CACTGGGTAT ATGTACAGCC ACAGCATCAA TATTGTGGCT
    ATTGTGGTGG AAGAAGGAAC AGGAGTAGAG GCCAATACTA CTCGGGATAT CTACATTTCT
    TCACAAATGG GATCAATAAC CTTTGAAGAC ACCAAAAATT TTTATTACCC CAATTTTCCC
    TTCAGTGGGA AGGTAAGAGT TAGGGGCCAT GATGGCTCCC TTCTCAAGAA TCATCCAGTA
    TTTTTGGTGA TTAATGGCAT AAATGGAACC ATCAACCAGA CCCTAACAAC TGACAATGAT
    GGCCTTGCTC CCTTCAAGCT GGATACAGTA AATTGGAATG GGAGAGATAT TTCTCTGGAG
    GCCAGATTCC AAATGGAAGA TTTGGTATAT AATCCAGGAG AGAGGCCTCA TTACTACCAA
    AATGATTATC TGCACCTGCA GGCCTTCTAC AACACAACTC AGAGCTTCCT TGGCATCCAC
    CCGCTAAGTG GACTCTTGGC ATGTAGCCAG CCCCAGGAAG TGCTGGTGGA CTATTACATT
    GATCCAGCTG ATGCAAACCC TGACCAGGAA ATCATCTTCT CCTACTATTT AATAGGGAAA
    GGGCACTTGG AGATGGAGGG ACAGAAACAC CTGAACTCTG AGAAGGAAGG AATGAAAGGC
    TCCTTCTCCC TCTCACTGAC CTTCAATTCC AGACTAGCCC CTGATCCTTC CCTAGTGGTC
    TATGCCATTT TTCCCAGTGG AGGCATTATA GCTGACAAAA TTCAGTTCTC AGTAGAGATG
    TGTTTTGACA ATCAGGTTTC CCTTGGCTTC TCACCTTCCA AGCAGCTTCC AGGAGCAGAT
    GTGGAACTAC AGCTACAGGC AGCTCCTGGA TCGCTGTGTG CAGTCCGGGC TGTGGATGAG
    AGTGTCTCAC TGCTTAGGCC GGAGAGAGAG CTAAGCAACA ACTCTGTCTA TAGGATGTTC
    TCATTCTGGT ATGGCCACTA CCCCTATCAG GTGGCTGAAT ATGATGAGTG TCCAGTGTCT
    GGCCCCTGGT ATGCTCCTCA GCCCCTCTCA GCTATGTGGA GGCCTTGGTT CTCTGAAGGC
    ATGGACCTTT TCCATCTTTT CCAGGGCATG GGCCTGAAAA TACTGTCCAA TGCCAAAATT
    AAGAAGCCAG TAGATTGCAT TCACCAGCCT CTGGAATACA GCTTTTCAAT GGGTGAAGCA
    AATTATAATA TTGGTACAGT GAATTCTGAG TCATCATCTT CTTCATCACT TCAGTCAGAG
    GACTCTCAGG TCCGCCAGTA CTTCCCGGAG ACCTGGCTCT GGGATCTGTT TCCTATTGGT
    GACTCAGGGA AGGAGGCTGT CCATCTTACA GTTCCTGATA CCATCACTGA GTGGAAGGCC
    ATGACTTTTT GTACCTCCCA GTCCAGCGGC TTTGGTCTTT CACCTACTGT TGGACTGACT
    GCTTTCAAGC CATTCTTTGT TGACCTGACT CTCCCTTACT CAGTAGTTCG TGGGGAATCC
    TTTCGCCTTA CTGCCACCAT CTTCAATTAT CTAAAGGACT GCATCAGGGT TCAGACTAAT
    CTGGCTACAT CAGATAAATA CCAGGTGGAA TCGTGGACAG ATTCTCAGGG ATCCAGCTGT
    CTCTGTGCTG ATGAAGCAAA AACGTATCAC TGGAATATCA CAGCTATCAA ATTGGGTCAT
    GTAAACTTTA CTATCACTAC TAAGATTCTG GACAGCAGTG AACTCTGTAG GGGGGAAAAG
    GGGTTCATTC CAGCAAAGGG CCAGAGTGAC ACACTTATCA AACCAGTTCT GGTCAAGCCT
    GAGGGAGTCC TTGTGGAGAA GACATACAGC TCATTGCTAT GCCCAAAAGG ACAAGTGGCT
    TCAGAATCTA TCTCCCTGGA GCTCCCTGTG GATGTTGTTC CTGATTCAAC CAAGGCTTAT
    GTTACAGTTC TGGGAGACAT TATGGGCACG GCCCTACAGA ACCTAGACAA TCTGGTGCAG
    ATGCCAAGGG GCTGTGGGGA GCAGAACATG GTCTTGTTTG CTCCCATAAT CTACGTCTTG
    CAGTATCTGG AGAGGGCAAG GCTGCTGACT GAAGAAATCA AGTCTCAGGC AGTGGGTTTC
    CTGAAAATAG GGTACCAGAA GGAGCTAATG TACAAACACA GCAACGGCTC ATACAGTGCC
    TTTGGGGAGC AGGATGGAGA TGGAAACACA TGGCTGACAG CGTTTGTCAC CAAATGCTTT
    GGCCAAGCTC AAGAATTTAT CTTCATTGAT GACAAGAATA TCCAGGATGC TCTCAAGTGG
    ATGGCGGGAA ACCAGCTCCC CAACGGTTGC TATGCTAATG TGGGAAACCT CATTCACACA
    GCTATGAAGG GCGGTGTTGA AGATGAGATT TCCCTGACTG CATACATCAC AGCTGCATTG
    CTGGAAATGG GAATGACCGC ACATGACCCA ATGGTGAGTC AGGGTCTGAA GTGCCTCCGA
    GACTCTGTTT CCTCTACTAC CAACCTCTAC ACACAGGGCC TCCTTGCTTA TACTTTCTCT
    CTGGCTGGGG AGATGGACAT CAGAAACGTT CTTCTTGAAA AGTTAGACCA GCAGGCTATC
    ATCTCAGGAG GGTCTATTCA CTGGAGCCAA AAGCCTATTC CGTCATTGGA TGACAGACCT
    TGGTCTCAGC CTGAGGCTGT AGATGTGGAA CTTACAGCAT ATATATTACT GGCCCAACTT
    AGCAAAGCCA GCCTGACTCA AAAGGAGATA GCCAAGGCCA CTGCCATAGT GGCTTGGTTA
    ACCAAGCAAC GTAATGCATA TGGGGGCTTC TCTTCTACTC AGGATACTGT AGTTGCTCTC
    CAAGCTCTTG CCAAATATGC CACTGTAGCC TATGTAACAT CTGAAAAAGT CAACCTGGCT
    GTAAAATCCA ATAAGAATTT CCAGCACACT TTCAGCATTC AGGCTGCCAA CCGGTTGGTG
    CTTCAGCAGG AAACTCTGCC CAGTATCCCT GGAGTGTACA CCTTGGAAGC CTCAGGCCAG
    GGCTGTGTCT ATGTGCAGAT GGTGCTGATA TACCATATTC CCCCTCCTAA GTTTGTGGAC
    ACCTTTAGTC TTAGTGTGGA AATGGGAAAA GCTAGATGTG AGCAAGCTAC TTCACCTCGA
    TCCTTGATGC TCACCATACA CACCAGTTAC GTGGGGAGTC GCAGTTCTTC CAATATGGCC
    ATTGTAGAAG TGAAGATGCT TTCTGGGTTC AGCCCCAAGG AGGGTACCAA TCAGTTACTT
    CTCCAACAAC CTCTGGTGAA GAAGGTGGAA TCTGGAACTG ATATCCTGAA CATTTATTTG
    GAAGAGCTGA GCAAGAAGAC TCAGACCTAT TCCTTTACCA TCAGCCAAAG TGTGTTGGTC
    ACTAACCTGA AACCAGCAAC CATCACAGTC TATGACTACT ACCTCCCAGA TGAACGGGCA
    ACAATTCAGT ACTCTGACCC CTGTGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe282941
    Clone ID Related Accession (Same CDS sequence) XM_022411342.1
    Accession Version XM_022411342.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3447bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product alpha-2-macroglobulin-like protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTCTACCT TCAACCTCAC TGGGTATATG TACAGCCACA GCATCAATAT TGTGGCTATT 
    GTGGTGGAAG AAGGAACAGG AGTAGAGGCC AATACTACTC GGGATATCTA CATTTCTTCA
    CAAATGGGAT CAATAACCTT TGAAGACACC AAAAATTTTT ATTACCCCAA TTTTCCCTTC
    AGTGGGAAGG TAAGAGTTAG GGGCCATGAT GGCTCCCTTC TCAAGAATCA TCCAGTATTT
    TTGGTGATTA ATGGCATAAA TGGAACCATC AACCAGACCC TAACAACTGA CAATGATGGC
    CTTGCTCCCT TCAAGCTGGA TACAGTAAAT TGGAATGGGA GAGATATTTC TCTGGAGGCC
    AGATTCCAAA TGGAAGATTT GGTATATAAT CCAGGAGAGA GGCCTCATTA CTACCAAAAT
    GATTATCTGC ACCTGCAGGC CTTCTACAAC ACAACTCAGA GCTTCCTTGG CATCCACCCG
    CTAAGTGGAC TCTTGGCATG TAGCCAGCCC CAGGAAGTGC TGGTGGACTA TTACATTGAT
    CCAGCTGATG CAAACCCTGA CCAGGAAATC ATCTTCTCCT ACTATTTAAT AGGGAAAGGG
    CACTTGGAGA TGGAGGGACA GAAACACCTG AACTCTGAGA AGGAAGGAAT GAAAGGCTCC
    TTCTCCCTCT CACTGACCTT CAATTCCAGA CTAGCCCCTG ATCCTTCCCT AGTGGTCTAT
    GCCATTTTTC CCAGTGGAGG CATTATAGCT GACAAAATTC AGTTCTCAGT AGAGATGTGT
    TTTGACAATC AGGTTTCCCT TGGCTTCTCA CCTTCCAAGC AGCTTCCAGG AGCAGATGTG
    GAACTACAGC TACAGGCAGC TCCTGGATCG CTGTGTGCAG TCCGGGCTGT GGATGAGAGT
    GTCTCACTGC TTAGGCCGGA GAGAGAGCTA AGCAACAACT CTGTCTATAG GATGTTCTCA
    TTCTGGTATG GCCACTACCC CTATCAGGTG GCTGAATATG ATGAGTGTCC AGTGTCTGGC
    CCCTGGTATG CTCCTCAGCC CCTCTCAGCT ATGTGGAGGC CTTGGTTCTC TGAAGGCATG
    GACCTTTTCC ATCTTTTCCA GGGCATGGGC CTGAAAATAC TGTCCAATGC CAAAATTAAG
    AAGCCAGTAG ATTGCATTCA CCAGCCTCTG GAATACAGCT TTTCAATGGG TGAAGCAAAT
    TATAATATTG GTACAGTGAA TTCTGAGTCA TCATCTTCTT CATCACTTCA GTCAGAGGAC
    TCTCAGGTCC GCCAGTACTT CCCGGAGACC TGGCTCTGGG ATCTGTTTCC TATTGGTGAC
    TCAGGGAAGG AGGCTGTCCA TCTTACAGTT CCTGATACCA TCACTGAGTG GAAGGCCATG
    ACTTTTTGTA CCTCCCAGTC CAGCGGCTTT GGTCTTTCAC CTACTGTTGG ACTGACTGCT
    TTCAAGCCAT TCTTTGTTGA CCTGACTCTC CCTTACTCAG TAGTTCGTGG GGAATCCTTT
    CGCCTTACTG CCACCATCTT CAATTATCTA AAGGACTGCA TCAGGGTTCA GACTAATCTG
    GCTACATCAG ATAAATACCA GGTGGAATCG TGGACAGATT CTCAGGGATC CAGCTGTCTC
    TGTGCTGATG AAGCAAAAAC GTATCACTGG AATATCACAG CTATCAAATT GGGTCATGTA
    AACTTTACTA TCACTACTAA GATTCTGGAC AGCAGTGAAC TCTGTAGGGG GGAAAAGGGG
    TTCATTCCAG CAAAGGGCCA GAGTGACACA CTTATCAAAC CAGTTCTGGT CAAGCCTGAG
    GGAGTCCTTG TGGAGAAGAC ATACAGCTCA TTGCTATGCC CAAAAGGACA AGTGGCTTCA
    GAATCTATCT CCCTGGAGCT CCCTGTGGAT GTTGTTCCTG ATTCAACCAA GGCTTATGTT
    ACAGTTCTGG GAGACATTAT GGGCACGGCC CTACAGAACC TAGACAATCT GGTGCAGATG
    CCAAGGGGCT GTGGGGAGCA GAACATGGTC TTGTTTGCTC CCATAATCTA CGTCTTGCAG
    TATCTGGAGA GGGCAAGGCT GCTGACTGAA GAAATCAAGT CTCAGGCAGT GGGTTTCCTG
    AAAATAGGGT ACCAGAAGGA GCTAATGTAC AAACACAGCA ACGGCTCATA CAGTGCCTTT
    GGGGAGCAGG ATGGAGATGG AAACACATGG CTGACAGCGT TTGTCACCAA ATGCTTTGGC
    CAAGCTCAAG AATTTATCTT CATTGATGAC AAGAATATCC AGGATGCTCT CAAGTGGATG
    GCGGGAAACC AGCTCCCCAA CGGTTGCTAT GCTAATGTGG GAAACCTCAT TCACACAGCT
    ATGAAGGGCG GTGTTGAAGA TGAGATTTCC CTGACTGCAT ACATCACAGC TGCATTGCTG
    GAAATGGGAA TGACCGCACA TGACCCAATG GTGAGTCAGG GTCTGAAGTG CCTCCGAGAC
    TCTGTTTCCT CTACTACCAA CCTCTACACA CAGGGCCTCC TTGCTTATAC TTTCTCTCTG
    GCTGGGGAGA TGGACATCAG AAACGTTCTT CTTGAAAAGT TAGACCAGCA GGCTATCATC
    TCAGGAGGGT CTATTCACTG GAGCCAAAAG CCTATTCCGT CATTGGATGA CAGACCTTGG
    TCTCAGCCTG AGGCTGTAGA TGTGGAACTT ACAGCATATA TATTACTGGC CCAACTTAGC
    AAAGCCAGCC TGACTCAAAA GGAGATAGCC AAGGCCACTG CCATAGTGGC TTGGTTAACC
    AAGCAACGTA ATGCATATGG GGGCTTCTCT TCTACTCAGG ATACTGTAGT TGCTCTCCAA
    GCTCTTGCCA AATATGCCAC TGTAGCCTAT GTAACATCTG AAAAAGTCAA CCTGGCTGTA
    AAATCCAATA AGAATTTCCA GCACACTTTC AGCATTCAGG CTGCCAACCG GTTGGTGCTT
    CAGCAGGAAA CTCTGCCCAG TATCCCTGGA GTGTACACCT TGGAAGCCTC AGGCCAGGGC
    TGTGTCTATG TGCAGATGGT GCTGATATAC CATATTCCCC CTCCTAAGTT TGTGGACACC
    TTTAGTCTTA GTGTGGAAAT GGGAAAAGCT AGATGTGAGC AAGCTACTTC ACCTCGATCC
    TTGATGCTCA CCATACACAC CAGTTACGTG GGGAGTCGCA GTTCTTCCAA TATGGCCATT
    GTAGAAGTGA AGATGCTTTC TGGGTTCAGC CCCAAGGAGG GTACCAATCA GTTACTTCTC
    CAACAACCTC TGGTGAAGAA GGTGGAATCT GGAACTGATA TCCTGAACAT TTATTTGGAA
    GAGCTGAGCA AGAAGACTCA GACCTATTCC TTTACCATCA GCCAAAGTGT GTTGGTCACT
    AACCTGAAAC CAGCAACCAT CACAGTCTAT GACTACTACC TCCCAGATGA ACGGGCAACA
    ATTCAGTACT CTGACCCCTG TGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022267050.1
    CDS84..3530
    Translation

    Target ORF information:

    RefSeq Version XM_022411342.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris alpha-2-macroglobulin like 1 (A2ML1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022411342.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTCTACCT TCAACCTCAC TGGGTATATG TACAGCCACA GCATCAATAT TGTGGCTATT 
    GTGGTGGAAG AAGGAACAGG AGTAGAGGCC AATACTACTC GGGATATCTA CATTTCTTCA
    CAAATGGGAT CAATAACCTT TGAAGACACC AAAAATTTTT ATTACCCCAA TTTTCCCTTC
    AGTGGGAAGG TAAGAGTTAG GGGCCATGAT GGCTCCCTTC TCAAGAATCA TCCAGTATTT
    TTGGTGATTA ATGGCATAAA TGGAACCATC AACCAGACCC TAACAACTGA CAATGATGGC
    CTTGCTCCCT TCAAGCTGGA TACAGTAAAT TGGAATGGGA GAGATATTTC TCTGGAGGCC
    AGATTCCAAA TGGAAGATTT GGTATATAAT CCAGGAGAGA GGCCTCATTA CTACCAAAAT
    GATTATCTGC ACCTGCAGGC CTTCTACAAC ACAACTCAGA GCTTCCTTGG CATCCACCCG
    CTAAGTGGAC TCTTGGCATG TAGCCAGCCC CAGGAAGTGC TGGTGGACTA TTACATTGAT
    CCAGCTGATG CAAACCCTGA CCAGGAAATC ATCTTCTCCT ACTATTTAAT AGGGAAAGGG
    CACTTGGAGA TGGAGGGACA GAAACACCTG AACTCTGAGA AGGAAGGAAT GAAAGGCTCC
    TTCTCCCTCT CACTGACCTT CAATTCCAGA CTAGCCCCTG ATCCTTCCCT AGTGGTCTAT
    GCCATTTTTC CCAGTGGAGG CATTATAGCT GACAAAATTC AGTTCTCAGT AGAGATGTGT
    TTTGACAATC AGGTTTCCCT TGGCTTCTCA CCTTCCAAGC AGCTTCCAGG AGCAGATGTG
    GAACTACAGC TACAGGCAGC TCCTGGATCG CTGTGTGCAG TCCGGGCTGT GGATGAGAGT
    GTCTCACTGC TTAGGCCGGA GAGAGAGCTA AGCAACAACT CTGTCTATAG GATGTTCTCA
    TTCTGGTATG GCCACTACCC CTATCAGGTG GCTGAATATG ATGAGTGTCC AGTGTCTGGC
    CCCTGGTATG CTCCTCAGCC CCTCTCAGCT ATGTGGAGGC CTTGGTTCTC TGAAGGCATG
    GACCTTTTCC ATCTTTTCCA GGGCATGGGC CTGAAAATAC TGTCCAATGC CAAAATTAAG
    AAGCCAGTAG ATTGCATTCA CCAGCCTCTG GAATACAGCT TTTCAATGGG TGAAGCAAAT
    TATAATATTG GTACAGTGAA TTCTGAGTCA TCATCTTCTT CATCACTTCA GTCAGAGGAC
    TCTCAGGTCC GCCAGTACTT CCCGGAGACC TGGCTCTGGG ATCTGTTTCC TATTGGTGAC
    TCAGGGAAGG AGGCTGTCCA TCTTACAGTT CCTGATACCA TCACTGAGTG GAAGGCCATG
    ACTTTTTGTA CCTCCCAGTC CAGCGGCTTT GGTCTTTCAC CTACTGTTGG ACTGACTGCT
    TTCAAGCCAT TCTTTGTTGA CCTGACTCTC CCTTACTCAG TAGTTCGTGG GGAATCCTTT
    CGCCTTACTG CCACCATCTT CAATTATCTA AAGGACTGCA TCAGGGTTCA GACTAATCTG
    GCTACATCAG ATAAATACCA GGTGGAATCG TGGACAGATT CTCAGGGATC CAGCTGTCTC
    TGTGCTGATG AAGCAAAAAC GTATCACTGG AATATCACAG CTATCAAATT GGGTCATGTA
    AACTTTACTA TCACTACTAA GATTCTGGAC AGCAGTGAAC TCTGTAGGGG GGAAAAGGGG
    TTCATTCCAG CAAAGGGCCA GAGTGACACA CTTATCAAAC CAGTTCTGGT CAAGCCTGAG
    GGAGTCCTTG TGGAGAAGAC ATACAGCTCA TTGCTATGCC CAAAAGGACA AGTGGCTTCA
    GAATCTATCT CCCTGGAGCT CCCTGTGGAT GTTGTTCCTG ATTCAACCAA GGCTTATGTT
    ACAGTTCTGG GAGACATTAT GGGCACGGCC CTACAGAACC TAGACAATCT GGTGCAGATG
    CCAAGGGGCT GTGGGGAGCA GAACATGGTC TTGTTTGCTC CCATAATCTA CGTCTTGCAG
    TATCTGGAGA GGGCAAGGCT GCTGACTGAA GAAATCAAGT CTCAGGCAGT GGGTTTCCTG
    AAAATAGGGT ACCAGAAGGA GCTAATGTAC AAACACAGCA ACGGCTCATA CAGTGCCTTT
    GGGGAGCAGG ATGGAGATGG AAACACATGG CTGACAGCGT TTGTCACCAA ATGCTTTGGC
    CAAGCTCAAG AATTTATCTT CATTGATGAC AAGAATATCC AGGATGCTCT CAAGTGGATG
    GCGGGAAACC AGCTCCCCAA CGGTTGCTAT GCTAATGTGG GAAACCTCAT TCACACAGCT
    ATGAAGGGCG GTGTTGAAGA TGAGATTTCC CTGACTGCAT ACATCACAGC TGCATTGCTG
    GAAATGGGAA TGACCGCACA TGACCCAATG GTGAGTCAGG GTCTGAAGTG CCTCCGAGAC
    TCTGTTTCCT CTACTACCAA CCTCTACACA CAGGGCCTCC TTGCTTATAC TTTCTCTCTG
    GCTGGGGAGA TGGACATCAG AAACGTTCTT CTTGAAAAGT TAGACCAGCA GGCTATCATC
    TCAGGAGGGT CTATTCACTG GAGCCAAAAG CCTATTCCGT CATTGGATGA CAGACCTTGG
    TCTCAGCCTG AGGCTGTAGA TGTGGAACTT ACAGCATATA TATTACTGGC CCAACTTAGC
    AAAGCCAGCC TGACTCAAAA GGAGATAGCC AAGGCCACTG CCATAGTGGC TTGGTTAACC
    AAGCAACGTA ATGCATATGG GGGCTTCTCT TCTACTCAGG ATACTGTAGT TGCTCTCCAA
    GCTCTTGCCA AATATGCCAC TGTAGCCTAT GTAACATCTG AAAAAGTCAA CCTGGCTGTA
    AAATCCAATA AGAATTTCCA GCACACTTTC AGCATTCAGG CTGCCAACCG GTTGGTGCTT
    CAGCAGGAAA CTCTGCCCAG TATCCCTGGA GTGTACACCT TGGAAGCCTC AGGCCAGGGC
    TGTGTCTATG TGCAGATGGT GCTGATATAC CATATTCCCC CTCCTAAGTT TGTGGACACC
    TTTAGTCTTA GTGTGGAAAT GGGAAAAGCT AGATGTGAGC AAGCTACTTC ACCTCGATCC
    TTGATGCTCA CCATACACAC CAGTTACGTG GGGAGTCGCA GTTCTTCCAA TATGGCCATT
    GTAGAAGTGA AGATGCTTTC TGGGTTCAGC CCCAAGGAGG GTACCAATCA GTTACTTCTC
    CAACAACCTC TGGTGAAGAA GGTGGAATCT GGAACTGATA TCCTGAACAT TTATTTGGAA
    GAGCTGAGCA AGAAGACTCA GACCTATTCC TTTACCATCA GCCAAAGTGT GTTGGTCACT
    AACCTGAAAC CAGCAACCAT CACAGTCTAT GACTACTACC TCCCAGATGA ACGGGCAACA
    ATTCAGTACT CTGACCCCTG TGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe43565
    Clone ID Related Accession (Same CDS sequence) XM_014108319.1
    Accession Version XM_014108319.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4371bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product alpha-2-macroglobulin-like protein 1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTTGGTG CTATGGTCAA CCTCACTAGG TACCCAGGTT TGGAGGCACA GGAAAAAGAG 
    AAGTTAGTTC TATCTGGAGC AGTGGAAACT ATGAGAAACT ACCTGGTGAC ATTACCAGCC
    AGGCTTAACT TCCCCTCTAC CCAGAAAGTT TGTTTGGATC TGAGCCCTGG GTCCCATGAT
    TTAAAATTCA CTATTACCCT GGAGACCAAG GATAAGACTC AGAAGTTGCT AGAAACCTCT
    GGATTGAAGA AGAGGCTCTT ACGCTGCACT TCCTTTCCGG TACCACCTCC TGCTGGTGGC
    ACAGAAGAAG TTGCTACAGT CTGGGTGTCC GGGACTGGAA ATAATATCAG CTTTGAGGAG
    AAGAAAACAG TTCTAATTGA GAGGGAGGGG AATGGCATCT TTATACAAAC GGACAAACCT
    ATCTACAGCC CAGGGCAGGA AGTGCACTTC CGCATTGTCA CCCTGAACAG CAGCTTTGTT
    CCAGTGAATG ACAAGTACTC CATGGTGGAA CTGCAGGATC CAAATAGCAA CAGGATTGCA
    CAGTGGCTGG AAGTGGTACC TCAGCAAGGC ATTGCAGACC TATCCTTCCA ACTGGCACCA
    GAGGCAATGC TGGGAACCTA TATGGTAGCA GTGGCTGGGG GCATGGCCTT TGGCACCTTC
    AGTGTGGAAG AATATGTGTT GCCTAAGTTT AAGGTGGATG TGGTGGAGCC CAAGCAGTTA
    TCAACAGTGG AAGAATCCTT CTTAGTCAAG ATTTGTTGTA GGTACACCTA CGGAAAGCCC
    ATGCTAGGGA CAGCACAGGT ATCAGTGTGT CAAAAGCCAT ATAGCTACCA TTTTCCAGAG
    CCAGAACAGC AACAGCTACC TGACAGATGC ACAAACCTTT CTGGACAGAC TGACAAAGCA
    GGATGCTTCT CAGCTTCTGT GGACATGTCT ACCTTCAACC TCACTGGGTA TATGTACAGC
    CACAGCATCA ATATTGTGGC TATTGTGGTG GAAGAAGGAA CAGGAGTAGA GGCCAATACT
    ACTCGGGATA TCTACATTTC TTCACAAATG GGATCAATAA CCTTTGAAGA CACCAAAAAT
    TTTTATTACC CCAATTTTCC CTTCAGTGGG AAGGTAAGAG TTAGGGGCCA TGATGGCTCC
    CTTCTCAAGA ATCATCCAGT ATTTTTGGTG ATTAATGGCA TAAATGGAAC CATCAACCAG
    ACCCTAACAA CTGACAATGA TGGCCTTGCT CCCTTCAAGC TGGATACAGT AAATTGGAAT
    GGGAGAGATA TTTCTCTGGA GGCCAGATTC CAAATGGAAG ATTTGGTATA TAATCCAGGA
    GAGAGGCCTC ATTACTACCA AAATGATTAT CTGCACCTGC AGGCCTTCTA CAACACAACT
    CAGAGCTTCC TTGGCATCCA CCCGCTAAGT GGACTCTTGG CATGTAGCCA GCCCCAGGAA
    GTGCTGGTGG ACTATTACAT TGATCCAGCT GATGCAAACC CTGACCAGGA AATCATCTTC
    TCCTACTATT TAATAGGGAA AGGGCACTTG GAGATGGAGG GACAGAAACA CCTGAACTCT
    GAGAAGGAAG GAATGAAAGG CTCCTTCTCC CTCTCACTGA CCTTCAATTC CAGACTAGCC
    CCTGATCCTT CCCTAGTGGT CTATGCCATT TTTCCCAGTG GAGGCATTAT AGCTGACAAA
    ATTCAGTTCT CAGTAGAGAT GTGTTTTGAC AATCAGGTTT CCCTTGGCTT CTCACCTTCC
    AAGCAGCTTC CAGGAGCAGA TGTGGAACTA CAGCTACAGG CAGCTCCTGG ATCGCTGTGT
    GCAGTCCGGG CTGTGGATGA GAGTGTCTCA CTGCTTAGGC CGGAGAGAGA GCTAAGCAAC
    AACTCTGTCT ATAGGATGTT CTCATTCTGG TATGGCCACT ACCCCTATCA GGTGGCTGAA
    TATGATGAGT GTCCAGTGTC TGGCCCCTGG TATGCTCCTC AGCCCCTCTC AGCTATGTGG
    AGGCCTTGGT TCTCTGAAGG CATGGACCTT TTCCATCTTT TCCAGGGCAT GGGCCTGAAA
    ATACTGTCCA ATGCCAAAAT TAAGAAGCCA GTAGATTGCA TTCACCAGCC TCTGGAATAC
    AGCTTTTCAA TGGGTGAAGC AAATTATAAT ATTGGTACAG TGAATTCTGA GTCATCATCT
    TCTTCATCAC TTCAGTCAGA GGACTCTCAG GTCCGCCAGT ACTTCCCGGA GACCTGGCTC
    TGGGATCTGT TTCCTATTGG TGACTCAGGG AAGGAGGCTG TCCATCTTAC AGTTCCTGAT
    ACCATCACTG AGTGGAAGGC CATGACTTTT TGTACCTCCC AGTCCAGCGG CTTTGGTCTT
    TCACCTACTG TTGGACTGAC TGCTTTCAAG CCATTCTTTG TTGACCTGAC TCTCCCTTAC
    TCAGTAGTTC GTGGGGAATC CTTTCGCCTT ACTGCCACCA TCTTCAATTA TCTAAAGGAC
    TGCATCAGGG TTCAGACTAA TCTGGCTACA TCAGATAAAT ACCAGGTGGA ATCGTGGACA
    GATTCTCAGG GATCCAGCTG TCTCTGTGCT GATGAAGCAA AAACGTATCA CTGGAATATC
    ACAGCTATCA AATTGGGTCA TGTAAACTTT ACTATCACTA CTAAGATTCT GGACAGCAGT
    GAACTCTGTA GGGGGGAAAA GGGGTTCATT CCAGCAAAGG GCCAGAGTGA CACACTTATC
    AAACCAGTTC TGGTCAAGCC TGAGGGAGTC CTTGTGGAGA AGACATACAG CTCATTGCTA
    TGCCCAAAAG GACAAGTGGC TTCAGAATCT ATCTCCCTGG AGCTCCCTGT GGATGTTGTT
    CCTGATTCAA CCAAGGCTTA TGTTACAGTT CTGGGAGACA TTATGGGCAC GGCCCTACAG
    AACCTAGACA ATCTGGTGCA GATGCCAAGG GGCTGTGGGG AGCAGAACAT GGTCTTGTTT
    GCTCCCATAA TCTACGTCTT GCAGTATCTG GAGAGGGCAA GGCTGCTGAC TGAAGAAATC
    AAGTCTCAGG CAGTGGGTTT CCTGAAAATA GGGTACCAGA AGGAGCTAAT GTACAAACAC
    AGCAACGGCT CATACAGTGC CTTTGGGGAG CAGGATGGAG ATGGAAACAC ATGGCTGACA
    GCGTTTGTCA CCAAATGCTT TGGCCAAGCT CAAGAATTTA TCTTCATTGA TGACAAGAAT
    ATCCAGGATG CTCTCAAGTG GATGGCGGGA AACCAGCTCC CCAACGGTTG CTATGCTAAT
    GTGGGAAACC TCATTCACAC AGCTATGAAG GGCGGTGTTG AAGATGAGAT TTCCCTGACT
    GCATACATCA CAGCTGCATT GCTGGAAATG GGAATGACCG CACATGACCC AATGGTGAGT
    CAGGGTCTGA AGTGCCTCCG AGACTCTGTT TCCTCTACTA CCAACCTCTA CACACAGGGC
    CTCCTTGCTT ATACTTTCTC TCTGGCTGGG GAGATGGACA TCAGAAACGT TCTTCTTGAA
    AAGTTAGACC AGCAGGCTAT CATCTCAGGA GGGTCTATTC ACTGGAGCCA AAAGCCTATT
    CCGTCATTGG ATGACAGACC TTGGTCTCAG CCTGAGGCTG TAGATGTGGA ACTTACAGCA
    TATATATTAC TGGCCCAACT TAGCAAAGCC AGCCTGACTC AAAAGGAGAT AGCCAAGGCC
    ACTGCCATAG TGGCTTGGTT AACCAAGCAA CGTAATGCAT ATGGGGGCTT CTCTTCTACT
    CAGGATACTG TAGTTGCTCT CCAAGCTCTT GCCAAATATG CCACTGTAGC CTATGTAACA
    TCTGAAAAAG TCAACCTGGC TGTAAAATCC AATAAGAATT TCCAGCACAC TTTCAGCATT
    CAGGCTGCCA ACCGGTTGGT GCTTCAGCAG GAAACTCTGC CCAGTATCCC TGGAGTGTAC
    ACCTTGGAAG CCTCAGGCCA GGGCTGTGTC TATGTGCAGA TGGTGCTGAT ATACCATATT
    CCCCCTCCTA AGTTTGTGGA CACCTTTAGT CTTAGTGTGG AAATGGGAAA AGCTAGATGT
    GAGCAAGCTA CTTCACCTCG ATCCTTGATG CTCACCATAC ACACCAGTTA CGTGGGGAGT
    CGCAGTTCTT CCAATATGGC CATTGTAGAA GTGAAGATGC TTTCTGGGTT CAGCCCCAAG
    GAGGGTACCA ATCAGTTACT TCTCCAACAA CCTCTGGTGA AGAAGGTGGA ATCTGGAACT
    GATATCCTGA ACATTTATTT GGAAGAGCTG AGCAAGAAGA CTCAGACCTA TTCCTTTACC
    ATCAGCCAAA GTGTGTTGGT CACTAACCTG AAACCAGCAA CCATCACAGT CTATGACTAC
    TACCTCCCAG ATGAACGGGC AACAATTCAG TACTCTGACC CCTGTGAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963794.1
    CDS1..4371
    Translation

    Target ORF information:

    RefSeq Version XM_014108319.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris alpha-2-macroglobulin-like 1 (A2ML1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108319.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGTTGGTG CTATGGTCAA CCTCACTAGG TACCCAGGTT TGGAGGCACA GGAAAAAGAG 
    AAGTTAGTTC TATCTGGAGC AGTGGAAACT ATGAGAAACT ACCTGGTGAC ATTACCAGCC
    AGGCTTAACT TCCCCTCTAC CCAGAAAGTT TGTTTGGATC TGAGCCCTGG GTCCCATGAT
    TTAAAATTCA CTATTACCCT GGAGACCAAG GATAAGACTC AGAAGTTGCT AGAAACCTCT
    GGATTGAAGA AGAGGCTCTT ACGCTGCACT TCCTTTCCGG TACCACCTCC TGCTGGTGGC
    ACAGAAGAAG TTGCTACAGT CTGGGTGTCC GGGACTGGAA ATAATATCAG CTTTGAGGAG
    AAGAAAACAG TTCTAATTGA GAGGGAGGGG AATGGCATCT TTATACAAAC GGACAAACCT
    ATCTACAGCC CAGGGCAGGA AGTGCACTTC CGCATTGTCA CCCTGAACAG CAGCTTTGTT
    CCAGTGAATG ACAAGTACTC CATGGTGGAA CTGCAGGATC CAAATAGCAA CAGGATTGCA
    CAGTGGCTGG AAGTGGTACC TCAGCAAGGC ATTGCAGACC TATCCTTCCA ACTGGCACCA
    GAGGCAATGC TGGGAACCTA TATGGTAGCA GTGGCTGGGG GCATGGCCTT TGGCACCTTC
    AGTGTGGAAG AATATGTGTT GCCTAAGTTT AAGGTGGATG TGGTGGAGCC CAAGCAGTTA
    TCAACAGTGG AAGAATCCTT CTTAGTCAAG ATTTGTTGTA GGTACACCTA CGGAAAGCCC
    ATGCTAGGGA CAGCACAGGT ATCAGTGTGT CAAAAGCCAT ATAGCTACCA TTTTCCAGAG
    CCAGAACAGC AACAGCTACC TGACAGATGC ACAAACCTTT CTGGACAGAC TGACAAAGCA
    GGATGCTTCT CAGCTTCTGT GGACATGTCT ACCTTCAACC TCACTGGGTA TATGTACAGC
    CACAGCATCA ATATTGTGGC TATTGTGGTG GAAGAAGGAA CAGGAGTAGA GGCCAATACT
    ACTCGGGATA TCTACATTTC TTCACAAATG GGATCAATAA CCTTTGAAGA CACCAAAAAT
    TTTTATTACC CCAATTTTCC CTTCAGTGGG AAGGTAAGAG TTAGGGGCCA TGATGGCTCC
    CTTCTCAAGA ATCATCCAGT ATTTTTGGTG ATTAATGGCA TAAATGGAAC CATCAACCAG
    ACCCTAACAA CTGACAATGA TGGCCTTGCT CCCTTCAAGC TGGATACAGT AAATTGGAAT
    GGGAGAGATA TTTCTCTGGA GGCCAGATTC CAAATGGAAG ATTTGGTATA TAATCCAGGA
    GAGAGGCCTC ATTACTACCA AAATGATTAT CTGCACCTGC AGGCCTTCTA CAACACAACT
    CAGAGCTTCC TTGGCATCCA CCCGCTAAGT GGACTCTTGG CATGTAGCCA GCCCCAGGAA
    GTGCTGGTGG ACTATTACAT TGATCCAGCT GATGCAAACC CTGACCAGGA AATCATCTTC
    TCCTACTATT TAATAGGGAA AGGGCACTTG GAGATGGAGG GACAGAAACA CCTGAACTCT
    GAGAAGGAAG GAATGAAAGG CTCCTTCTCC CTCTCACTGA CCTTCAATTC CAGACTAGCC
    CCTGATCCTT CCCTAGTGGT CTATGCCATT TTTCCCAGTG GAGGCATTAT AGCTGACAAA
    ATTCAGTTCT CAGTAGAGAT GTGTTTTGAC AATCAGGTTT CCCTTGGCTT CTCACCTTCC
    AAGCAGCTTC CAGGAGCAGA TGTGGAACTA CAGCTACAGG CAGCTCCTGG ATCGCTGTGT
    GCAGTCCGGG CTGTGGATGA GAGTGTCTCA CTGCTTAGGC CGGAGAGAGA GCTAAGCAAC
    AACTCTGTCT ATAGGATGTT CTCATTCTGG TATGGCCACT ACCCCTATCA GGTGGCTGAA
    TATGATGAGT GTCCAGTGTC TGGCCCCTGG TATGCTCCTC AGCCCCTCTC AGCTATGTGG
    AGGCCTTGGT TCTCTGAAGG CATGGACCTT TTCCATCTTT TCCAGGGCAT GGGCCTGAAA
    ATACTGTCCA ATGCCAAAAT TAAGAAGCCA GTAGATTGCA TTCACCAGCC TCTGGAATAC
    AGCTTTTCAA TGGGTGAAGC AAATTATAAT ATTGGTACAG TGAATTCTGA GTCATCATCT
    TCTTCATCAC TTCAGTCAGA GGACTCTCAG GTCCGCCAGT ACTTCCCGGA GACCTGGCTC
    TGGGATCTGT TTCCTATTGG TGACTCAGGG AAGGAGGCTG TCCATCTTAC AGTTCCTGAT
    ACCATCACTG AGTGGAAGGC CATGACTTTT TGTACCTCCC AGTCCAGCGG CTTTGGTCTT
    TCACCTACTG TTGGACTGAC TGCTTTCAAG CCATTCTTTG TTGACCTGAC TCTCCCTTAC
    TCAGTAGTTC GTGGGGAATC CTTTCGCCTT ACTGCCACCA TCTTCAATTA TCTAAAGGAC
    TGCATCAGGG TTCAGACTAA TCTGGCTACA TCAGATAAAT ACCAGGTGGA ATCGTGGACA
    GATTCTCAGG GATCCAGCTG TCTCTGTGCT GATGAAGCAA AAACGTATCA CTGGAATATC
    ACAGCTATCA AATTGGGTCA TGTAAACTTT ACTATCACTA CTAAGATTCT GGACAGCAGT
    GAACTCTGTA GGGGGGAAAA GGGGTTCATT CCAGCAAAGG GCCAGAGTGA CACACTTATC
    AAACCAGTTC TGGTCAAGCC TGAGGGAGTC CTTGTGGAGA AGACATACAG CTCATTGCTA
    TGCCCAAAAG GACAAGTGGC TTCAGAATCT ATCTCCCTGG AGCTCCCTGT GGATGTTGTT
    CCTGATTCAA CCAAGGCTTA TGTTACAGTT CTGGGAGACA TTATGGGCAC GGCCCTACAG
    AACCTAGACA ATCTGGTGCA GATGCCAAGG GGCTGTGGGG AGCAGAACAT GGTCTTGTTT
    GCTCCCATAA TCTACGTCTT GCAGTATCTG GAGAGGGCAA GGCTGCTGAC TGAAGAAATC
    AAGTCTCAGG CAGTGGGTTT CCTGAAAATA GGGTACCAGA AGGAGCTAAT GTACAAACAC
    AGCAACGGCT CATACAGTGC CTTTGGGGAG CAGGATGGAG ATGGAAACAC ATGGCTGACA
    GCGTTTGTCA CCAAATGCTT TGGCCAAGCT CAAGAATTTA TCTTCATTGA TGACAAGAAT
    ATCCAGGATG CTCTCAAGTG GATGGCGGGA AACCAGCTCC CCAACGGTTG CTATGCTAAT
    GTGGGAAACC TCATTCACAC AGCTATGAAG GGCGGTGTTG AAGATGAGAT TTCCCTGACT
    GCATACATCA CAGCTGCATT GCTGGAAATG GGAATGACCG CACATGACCC AATGGTGAGT
    CAGGGTCTGA AGTGCCTCCG AGACTCTGTT TCCTCTACTA CCAACCTCTA CACACAGGGC
    CTCCTTGCTT ATACTTTCTC TCTGGCTGGG GAGATGGACA TCAGAAACGT TCTTCTTGAA
    AAGTTAGACC AGCAGGCTAT CATCTCAGGA GGGTCTATTC ACTGGAGCCA AAAGCCTATT
    CCGTCATTGG ATGACAGACC TTGGTCTCAG CCTGAGGCTG TAGATGTGGA ACTTACAGCA
    TATATATTAC TGGCCCAACT TAGCAAAGCC AGCCTGACTC AAAAGGAGAT AGCCAAGGCC
    ACTGCCATAG TGGCTTGGTT AACCAAGCAA CGTAATGCAT ATGGGGGCTT CTCTTCTACT
    CAGGATACTG TAGTTGCTCT CCAAGCTCTT GCCAAATATG CCACTGTAGC CTATGTAACA
    TCTGAAAAAG TCAACCTGGC TGTAAAATCC AATAAGAATT TCCAGCACAC TTTCAGCATT
    CAGGCTGCCA ACCGGTTGGT GCTTCAGCAG GAAACTCTGC CCAGTATCCC TGGAGTGTAC
    ACCTTGGAAG CCTCAGGCCA GGGCTGTGTC TATGTGCAGA TGGTGCTGAT ATACCATATT
    CCCCCTCCTA AGTTTGTGGA CACCTTTAGT CTTAGTGTGG AAATGGGAAA AGCTAGATGT
    GAGCAAGCTA CTTCACCTCG ATCCTTGATG CTCACCATAC ACACCAGTTA CGTGGGGAGT
    CGCAGTTCTT CCAATATGGC CATTGTAGAA GTGAAGATGC TTTCTGGGTT CAGCCCCAAG
    GAGGGTACCA ATCAGTTACT TCTCCAACAA CCTCTGGTGA AGAAGGTGGA ATCTGGAACT
    GATATCCTGA ACATTTATTT GGAAGAGCTG AGCAAGAAGA CTCAGACCTA TTCCTTTACC
    ATCAGCCAAA GTGTGTTGGT CACTAACCTG AAACCAGCAA CCATCACAGT CTATGACTAC
    TACCTCCCAG ATGAACGGGC AACAATTCAG TACTCTGACC CCTGTGAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.