EFHB cDNA ORF clone, Canis lupus familiaris(Dog)

The following EFHB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EFHB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe24087 XM_542768.6
Latest version!
Canis lupus familiaris EF-hand domain family member B (EFHB), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe24088 XM_005634369.3
Latest version!
Canis lupus familiaris EF-hand domain family member B (EFHB), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe24089 XM_005634370.2
Latest version!
Canis lupus familiaris EF-hand domain family member B (EFHB), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe42486 XM_014106569.2
Latest version!
Canis lupus familiaris EF-hand domain family member B (EFHB), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
View Old Accession Versions >>
OCe42486 XM_014106569.1 Canis lupus familiaris EF-hand domain family, member B (EFHB), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe24089 XM_005634370.1 Canis lupus familiaris EF-hand domain family, member B (EFHB), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe24087 XM_542768.5 Canis lupus familiaris EF-hand domain family, member B (EFHB), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe24088 XM_005634369.2 Canis lupus familiaris EF-hand domain family, member B (EFHB), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe24087
    Clone ID Related Accession (Same CDS sequence) XM_542768.6 , XM_542768.5
    Accession Version XM_542768.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2646bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product EF-hand domain-containing family member B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006605.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_542768.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGTGTTCTT TTGTGAGACT GGGGTCCCCT AAAGGGTTGA AGCACCACAA GATATTAGGA 
    ACTTCCCTTG AGCCAGTTAC AGCTTTTCCT TTAGAGATGA GTGACGTTAA AGGAATGAGG
    GTGTCAGAGA GGAAATCTTC TCGGAGCATG GAGGTTGGGC TCACCCACGA AGGAAAGGAT
    GACTTAGGAG AGACGGGGGT CCTTATGGGA GCAAGATCTC CCATAAGGGA GTTAGGGTTC
    AGAGTGGGAT TAGGAAAAGA AGATTCCTCA TGCAGGGAGA GCCTCCTGGT TGGTAACAAA
    TTTGAAGAAA GGATAACATC ACCAGAAACA AAATTTCCAT TGGGGATGGA GCTTGAAATG
    AAATTAAAAA GACAGAATCT TTCTGGAACC CTCCCGGAGA GGAGTAATTT AGGAGTCTAT
    GGGGTCTCAG CCTCCCAGGA AACAAAATCT TCTCCAGGTA TGTTGCCAGG AAGAAAGGGT
    GTAGAGAATG AGTATCTTAT GGCAGGATGT TCTGGAAGGA TAATACAGCC TCACTTGGGC
    ATGGTATGTG AAAATCCACA GACTCTAAGG AGCAGGAAAG GTTCTGTGGC TGGTTCCTCT
    GAAGGGTTAA GGGCTTCACT GGAGATGCAG CCTACTTTGG ATATGGAACC TAGACAGGAA
    CTGGAAAAAG AGACTTGTGT GGTGGTGAAG CCCAGTGCAG AGATGAAGCC TCCTGTGGAT
    GTGGAGGTGG ACACTGGTCT ACCCAAACTA GAAGAGCCAG ATGAAACAGG GCCCCAAATA
    GGTTTGGTGA TAGAACCTAC TGAGAGCCAG TTTGCCCAAC AACCTGAGGA GAGAAAGGAG
    GCTGAAAACA CTGAACCAGG AGTGGAGCCT CCAGATCGCA TCAGACCGGT ATACTCTGGG
    AAATTTTTTG ATCGGATGCC TTGCTGGCCA AGTGCAGGGA AAGTTATTCC TATTGGATAC
    AGAGTTGCAA CCTGTTTGAC TGAAAAACTT CCCAGGCTAA TTACTCCACC TGAGGCAAAA
    AAATTTTTCA ACTATAGAAA CCCACCAGCT GGAGCCGAAA GAGTATTTTA TGGCAGAGCA
    AATGATCCTC AGATTGCTCC TTATTTGACA CATGGAATAA GATCTAGAAT TTCAATACCG
    GCAAAGGTAC TGATAAACCC ACAGCCTGTA ACGACATTTC AACAGAAAAT TAAAGATAAA
    AAGGAATCAA TATATTTTAG TAATCAACGA GCACCACTAG GGAAATCTCA TGATCAAACG
    CCAGGATTAC CTAAAGGTCT GGATGTCCTC AATATGACAT TTGGGACAGC AGTCATCAGA
    GAATTATCTG CTAGAGATGT AGTGAATCCA CCAAAACCCT ATGAAGAAGT AGTTAAAGAA
    GGAAAAGAAG GACATGATCT GTATATTGTT TCTCACAATG ATTATTATGC AGGAGAAGCA
    AAGAACCGAA AGTATAACCC ATCAAGTTTC CATAGGTTTA ACTTGTATGG TGTCCCAACA
    CCACATTTTA ATGATGGAAG AGCCATGGCA AAAACTTTAT ATTGGCTCCA TGACCTACAA
    ATGAAAAGAG GAGCTAAGTT TGTATCCAAG AGAGTTGATG ATTTCAAAGA AAAGTTTCAA
    CATGAACTTG GAAGAGTTTT AGATCCCATT GCAGAAACAA TGAATATTCC CCCAGACCAC
    ACATTTGGAG CTTGTCTCCA TGCTGAAGAG TATGGAGCTG GTGATCTCAT CCATAACAGA
    CTTCCAAGTG AATATCTTCA AGGCAAGGAT AGACAGAGAG CCCTGATTGC AGCAGTGCGA
    CATCACTTGA AGAAAGTTAA CTACCAAAAC TTTGACACTT TGCTGGCAGC CTTCAGGCAT
    TATGATAAGA ATGGAGATGG GGTGATAGAT AAAGCCGAGC TTCGGGAGGC TTGTGAACAG
    GTCAGCTTGC ACTTAGATGA GAAGCTCCTG GACCAGCTAT TTGACTACTG TGATGTGGAT
    AATGATGGGC TGATTAACTA CCTAGAATTT GCAAATTTTC TTAACTGGAA GGATAAAATG
    CCTCTTAAAG AGTATGAAGA GAGGGTCATA ATTAAAGGTA GAAAACCAGA TTGTACAAAC
    CGTACTGAGG CTAATGTGGA AGAATCTGAA CCAACTCTCC TAATAAAACC AGAAGATATA
    GTCTTAAAAG AACCAGGGAG CTCAGAAAAG ACTCTCCGGA CGCTTCTGAG GCCAAGTGAT
    AAAGTTTCTA ATTACTATAA GACAACTTCT TCTGAGATCA GTGCGGTTGT AGGAGCTATT
    CCTTCTACTT GTTATCCCAT CTATGGTGTT CCAACCATTC GGTCTGATAT TCCTGCCCCC
    CGAATTCGTC GCATCAGTGA TAGAACTAAT TATGGTGAAG AGGGCAATGC CTATTCACTA
    CTACATCCTA CTGTCTTTGG CCAGAAAGGA GTGTTTGAAA GAGACTTCTT CAAGACCAGA
    TCAAAAAAAG AGATTGCAGA GATATTATGT AACATTGGTG TTAAGCTTTC TGATGAAGAA
    TTTGAAAATG TATGGAATCT TGCATCAAAA AAACACCACA GAGGAGAAGT TTGTGTTGAA
    ACCATCAGAA ATGTTCTGGA TGAGCTACAA CATGCAGACA GAATCAAGTG TAAAACAAGC
    ATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_542768.4
    CDS216..2861
    Translation

    Target ORF information:

    RefSeq Version XM_542768.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris EF-hand domain family member B (EFHB), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_542768.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGTGTTCTT TTGTGAGACT GGGGTCCCCT AAAGGGTTGA AGCACCACAA GATATTAGGA 
    ACTTCCCTTG AGCCAGTTAC AGCTTTTCCT TTAGAGATGA GTGACGTTAA AGGAATGAGG
    GTGTCAGAGA GGAAATCTTC TCGGAGCATG GAGGTTGGGC TCACCCACGA AGGAAAGGAT
    GACTTAGGAG AGACGGGGGT CCTTATGGGA GCAAGATCTC CCATAAGGGA GTTAGGGTTC
    AGAGTGGGAT TAGGAAAAGA AGATTCCTCA TGCAGGGAGA GCCTCCTGGT TGGTAACAAA
    TTTGAAGAAA GGATAACATC ACCAGAAACA AAATTTCCAT TGGGGATGGA GCTTGAAATG
    AAATTAAAAA GACAGAATCT TTCTGGAACC CTCCCGGAGA GGAGTAATTT AGGAGTCTAT
    GGGGTCTCAG CCTCCCAGGA AACAAAATCT TCTCCAGGTA TGTTGCCAGG AAGAAAGGGT
    GTAGAGAATG AGTATCTTAT GGCAGGATGT TCTGGAAGGA TAATACAGCC TCACTTGGGC
    ATGGTATGTG AAAATCCACA GACTCTAAGG AGCAGGAAAG GTTCTGTGGC TGGTTCCTCT
    GAAGGGTTAA GGGCTTCACT GGAGATGCAG CCTACTTTGG ATATGGAACC TAGACAGGAA
    CTGGAAAAAG AGACTTGTGT GGTGGTGAAG CCCAGTGCAG AGATGAAGCC TCCTGTGGAT
    GTGGAGGTGG ACACTGGTCT ACCCAAACTA GAAGAGCCAG ATGAAACAGG GCCCCAAATA
    GGTTTGGTGA TAGAACCTAC TGAGAGCCAG TTTGCCCAAC AACCTGAGGA GAGAAAGGAG
    GCTGAAAACA CTGAACCAGG AGTGGAGCCT CCAGATCGCA TCAGACCGGT ATACTCTGGG
    AAATTTTTTG ATCGGATGCC TTGCTGGCCA AGTGCAGGGA AAGTTATTCC TATTGGATAC
    AGAGTTGCAA CCTGTTTGAC TGAAAAACTT CCCAGGCTAA TTACTCCACC TGAGGCAAAA
    AAATTTTTCA ACTATAGAAA CCCACCAGCT GGAGCCGAAA GAGTATTTTA TGGCAGAGCA
    AATGATCCTC AGATTGCTCC TTATTTGACA CATGGAATAA GATCTAGAAT TTCAATACCG
    GCAAAGGTAC TGATAAACCC ACAGCCTGTA ACGACATTTC AACAGAAAAT TAAAGATAAA
    AAGGAATCAA TATATTTTAG TAATCAACGA GCACCACTAG GGAAATCTCA TGATCAAACG
    CCAGGATTAC CTAAAGGTCT GGATGTCCTC AATATGACAT TTGGGACAGC AGTCATCAGA
    GAATTATCTG CTAGAGATGT AGTGAATCCA CCAAAACCCT ATGAAGAAGT AGTTAAAGAA
    GGAAAAGAAG GACATGATCT GTATATTGTT TCTCACAATG ATTATTATGC AGGAGAAGCA
    AAGAACCGAA AGTATAACCC ATCAAGTTTC CATAGGTTTA ACTTGTATGG TGTCCCAACA
    CCACATTTTA ATGATGGAAG AGCCATGGCA AAAACTTTAT ATTGGCTCCA TGACCTACAA
    ATGAAAAGAG GAGCTAAGTT TGTATCCAAG AGAGTTGATG ATTTCAAAGA AAAGTTTCAA
    CATGAACTTG GAAGAGTTTT AGATCCCATT GCAGAAACAA TGAATATTCC CCCAGACCAC
    ACATTTGGAG CTTGTCTCCA TGCTGAAGAG TATGGAGCTG GTGATCTCAT CCATAACAGA
    CTTCCAAGTG AATATCTTCA AGGCAAGGAT AGACAGAGAG CCCTGATTGC AGCAGTGCGA
    CATCACTTGA AGAAAGTTAA CTACCAAAAC TTTGACACTT TGCTGGCAGC CTTCAGGCAT
    TATGATAAGA ATGGAGATGG GGTGATAGAT AAAGCCGAGC TTCGGGAGGC TTGTGAACAG
    GTCAGCTTGC ACTTAGATGA GAAGCTCCTG GACCAGCTAT TTGACTACTG TGATGTGGAT
    AATGATGGGC TGATTAACTA CCTAGAATTT GCAAATTTTC TTAACTGGAA GGATAAAATG
    CCTCTTAAAG AGTATGAAGA GAGGGTCATA ATTAAAGGTA GAAAACCAGA TTGTACAAAC
    CGTACTGAGG CTAATGTGGA AGAATCTGAA CCAACTCTCC TAATAAAACC AGAAGATATA
    GTCTTAAAAG AACCAGGGAG CTCAGAAAAG ACTCTCCGGA CGCTTCTGAG GCCAAGTGAT
    AAAGTTTCTA ATTACTATAA GACAACTTCT TCTGAGATCA GTGCGGTTGT AGGAGCTATT
    CCTTCTACTT GTTATCCCAT CTATGGTGTT CCAACCATTC GGTCTGATAT TCCTGCCCCC
    CGAATTCGTC GCATCAGTGA TAGAACTAAT TATGGTGAAG AGGGCAATGC CTATTCACTA
    CTACATCCTA CTGTCTTTGG CCAGAAAGGA GTGTTTGAAA GAGACTTCTT CAAGACCAGA
    TCAAAAAAAG AGATTGCAGA GATATTATGT AACATTGGTG TTAAGCTTTC TGATGAAGAA
    TTTGAAAATG TATGGAATCT TGCATCAAAA AAACACCACA GAGGAGAAGT TTGTGTTGAA
    ACCATCAGAA ATGTTCTGGA TGAGCTACAA CATGCAGACA GAATCAAGTG TAAAACAAGC
    ATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe24087
    Clone ID Related Accession (Same CDS sequence) XM_542768.6 , XM_542768.5
    Accession Version XM_542768.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2646bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product EF-hand domain-containing family member B isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726100.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_542768.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGTGTTCTT TTGTGAGACT GGGGTCCCCT AAAGGGTTGA AGCACCACAA GATATTAGGA 
    ACTTCCCTTG AGCCAGTTAC AGCTTTTCCT TTAGAGATGA GTGACGTTAA AGGAATGAGG
    GTGTCAGAGA GGAAATCTTC TCGGAGCATG GAGGTTGGGC TCACCCACGA AGGAAAGGAT
    GACTTAGGAG AGACGGGGGT CCTTATGGGA GCAAGATCTC CCATAAGGGA GTTAGGGTTC
    AGAGTGGGAT TAGGAAAAGA AGATTCCTCA TGCAGGGAGA GCCTCCTGGT TGGTAACAAA
    TTTGAAGAAA GGATAACATC ACCAGAAACA AAATTTCCAT TGGGGATGGA GCTTGAAATG
    AAATTAAAAA GACAGAATCT TTCTGGAACC CTCCCGGAGA GGAGTAATTT AGGAGTCTAT
    GGGGTCTCAG CCTCCCAGGA AACAAAATCT TCTCCAGGTA TGTTGCCAGG AAGAAAGGGT
    GTAGAGAATG AGTATCTTAT GGCAGGATGT TCTGGAAGGA TAATACAGCC TCACTTGGGC
    ATGGTATGTG AAAATCCACA GACTCTAAGG AGCAGGAAAG GTTCTGTGGC TGGTTCCTCT
    GAAGGGTTAA GGGCTTCACT GGAGATGCAG CCTACTTTGG ATATGGAACC TAGACAGGAA
    CTGGAAAAAG AGACTTGTGT GGTGGTGAAG CCCAGTGCAG AGATGAAGCC TCCTGTGGAT
    GTGGAGGTGG ACACTGGTCT ACCCAAACTA GAAGAGCCAG ATGAAACAGG GCCCCAAATA
    GGTTTGGTGA TAGAACCTAC TGAGAGCCAG TTTGCCCAAC AACCTGAGGA GAGAAAGGAG
    GCTGAAAACA CTGAACCAGG AGTGGAGCCT CCAGATCGCA TCAGACCGGT ATACTCTGGG
    AAATTTTTTG ATCGGATGCC TTGCTGGCCA AGTGCAGGGA AAGTTATTCC TATTGGATAC
    AGAGTTGCAA CCTGTTTGAC TGAAAAACTT CCCAGGCTAA TTACTCCACC TGAGGCAAAA
    AAATTTTTCA ACTATAGAAA CCCACCAGCT GGAGCCGAAA GAGTATTTTA TGGCAGAGCA
    AATGATCCTC AGATTGCTCC TTATTTGACA CATGGAATAA GATCTAGAAT TTCAATACCG
    GCAAAGGTAC TGATAAACCC ACAGCCTGTA ACGACATTTC AACAGAAAAT TAAAGATAAA
    AAGGAATCAA TATATTTTAG TAATCAACGA GCACCACTAG GGAAATCTCA TGATCAAACG
    CCAGGATTAC CTAAAGGTCT GGATGTCCTC AATATGACAT TTGGGACAGC AGTCATCAGA
    GAATTATCTG CTAGAGATGT AGTGAATCCA CCAAAACCCT ATGAAGAAGT AGTTAAAGAA
    GGAAAAGAAG GACATGATCT GTATATTGTT TCTCACAATG ATTATTATGC AGGAGAAGCA
    AAGAACCGAA AGTATAACCC ATCAAGTTTC CATAGGTTTA ACTTGTATGG TGTCCCAACA
    CCACATTTTA ATGATGGAAG AGCCATGGCA AAAACTTTAT ATTGGCTCCA TGACCTACAA
    ATGAAAAGAG GAGCTAAGTT TGTATCCAAG AGAGTTGATG ATTTCAAAGA AAAGTTTCAA
    CATGAACTTG GAAGAGTTTT AGATCCCATT GCAGAAACAA TGAATATTCC CCCAGACCAC
    ACATTTGGAG CTTGTCTCCA TGCTGAAGAG TATGGAGCTG GTGATCTCAT CCATAACAGA
    CTTCCAAGTG AATATCTTCA AGGCAAGGAT AGACAGAGAG CCCTGATTGC AGCAGTGCGA
    CATCACTTGA AGAAAGTTAA CTACCAAAAC TTTGACACTT TGCTGGCAGC CTTCAGGCAT
    TATGATAAGA ATGGAGATGG GGTGATAGAT AAAGCCGAGC TTCGGGAGGC TTGTGAACAG
    GTCAGCTTGC ACTTAGATGA GAAGCTCCTG GACCAGCTAT TTGACTACTG TGATGTGGAT
    AATGATGGGC TGATTAACTA CCTAGAATTT GCAAATTTTC TTAACTGGAA GGATAAAATG
    CCTCTTAAAG AGTATGAAGA GAGGGTCATA ATTAAAGGTA GAAAACCAGA TTGTACAAAC
    CGTACTGAGG CTAATGTGGA AGAATCTGAA CCAACTCTCC TAATAAAACC AGAAGATATA
    GTCTTAAAAG AACCAGGGAG CTCAGAAAAG ACTCTCCGGA CGCTTCTGAG GCCAAGTGAT
    AAAGTTTCTA ATTACTATAA GACAACTTCT TCTGAGATCA GTGCGGTTGT AGGAGCTATT
    CCTTCTACTT GTTATCCCAT CTATGGTGTT CCAACCATTC GGTCTGATAT TCCTGCCCCC
    CGAATTCGTC GCATCAGTGA TAGAACTAAT TATGGTGAAG AGGGCAATGC CTATTCACTA
    CTACATCCTA CTGTCTTTGG CCAGAAAGGA GTGTTTGAAA GAGACTTCTT CAAGACCAGA
    TCAAAAAAAG AGATTGCAGA GATATTATGT AACATTGGTG TTAAGCTTTC TGATGAAGAA
    TTTGAAAATG TATGGAATCT TGCATCAAAA AAACACCACA GAGGAGAAGT TTGTGTTGAA
    ACCATCAGAA ATGTTCTGGA TGAGCTACAA CATGCAGACA GAATCAAGTG TAAAACAAGC
    ATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_542768.4
    CDS79..2724
    Translation

    Target ORF information:

    RefSeq Version XM_542768.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris EF-hand domain family, member B (EFHB), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_542768.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGTGTTCTT TTGTGAGACT GGGGTCCCCT AAAGGGTTGA AGCACCACAA GATATTAGGA 
    ACTTCCCTTG AGCCAGTTAC AGCTTTTCCT TTAGAGATGA GTGACGTTAA AGGAATGAGG
    GTGTCAGAGA GGAAATCTTC TCGGAGCATG GAGGTTGGGC TCACCCACGA AGGAAAGGAT
    GACTTAGGAG AGACGGGGGT CCTTATGGGA GCAAGATCTC CCATAAGGGA GTTAGGGTTC
    AGAGTGGGAT TAGGAAAAGA AGATTCCTCA TGCAGGGAGA GCCTCCTGGT TGGTAACAAA
    TTTGAAGAAA GGATAACATC ACCAGAAACA AAATTTCCAT TGGGGATGGA GCTTGAAATG
    AAATTAAAAA GACAGAATCT TTCTGGAACC CTCCCGGAGA GGAGTAATTT AGGAGTCTAT
    GGGGTCTCAG CCTCCCAGGA AACAAAATCT TCTCCAGGTA TGTTGCCAGG AAGAAAGGGT
    GTAGAGAATG AGTATCTTAT GGCAGGATGT TCTGGAAGGA TAATACAGCC TCACTTGGGC
    ATGGTATGTG AAAATCCACA GACTCTAAGG AGCAGGAAAG GTTCTGTGGC TGGTTCCTCT
    GAAGGGTTAA GGGCTTCACT GGAGATGCAG CCTACTTTGG ATATGGAACC TAGACAGGAA
    CTGGAAAAAG AGACTTGTGT GGTGGTGAAG CCCAGTGCAG AGATGAAGCC TCCTGTGGAT
    GTGGAGGTGG ACACTGGTCT ACCCAAACTA GAAGAGCCAG ATGAAACAGG GCCCCAAATA
    GGTTTGGTGA TAGAACCTAC TGAGAGCCAG TTTGCCCAAC AACCTGAGGA GAGAAAGGAG
    GCTGAAAACA CTGAACCAGG AGTGGAGCCT CCAGATCGCA TCAGACCGGT ATACTCTGGG
    AAATTTTTTG ATCGGATGCC TTGCTGGCCA AGTGCAGGGA AAGTTATTCC TATTGGATAC
    AGAGTTGCAA CCTGTTTGAC TGAAAAACTT CCCAGGCTAA TTACTCCACC TGAGGCAAAA
    AAATTTTTCA ACTATAGAAA CCCACCAGCT GGAGCCGAAA GAGTATTTTA TGGCAGAGCA
    AATGATCCTC AGATTGCTCC TTATTTGACA CATGGAATAA GATCTAGAAT TTCAATACCG
    GCAAAGGTAC TGATAAACCC ACAGCCTGTA ACGACATTTC AACAGAAAAT TAAAGATAAA
    AAGGAATCAA TATATTTTAG TAATCAACGA GCACCACTAG GGAAATCTCA TGATCAAACG
    CCAGGATTAC CTAAAGGTCT GGATGTCCTC AATATGACAT TTGGGACAGC AGTCATCAGA
    GAATTATCTG CTAGAGATGT AGTGAATCCA CCAAAACCCT ATGAAGAAGT AGTTAAAGAA
    GGAAAAGAAG GACATGATCT GTATATTGTT TCTCACAATG ATTATTATGC AGGAGAAGCA
    AAGAACCGAA AGTATAACCC ATCAAGTTTC CATAGGTTTA ACTTGTATGG TGTCCCAACA
    CCACATTTTA ATGATGGAAG AGCCATGGCA AAAACTTTAT ATTGGCTCCA TGACCTACAA
    ATGAAAAGAG GAGCTAAGTT TGTATCCAAG AGAGTTGATG ATTTCAAAGA AAAGTTTCAA
    CATGAACTTG GAAGAGTTTT AGATCCCATT GCAGAAACAA TGAATATTCC CCCAGACCAC
    ACATTTGGAG CTTGTCTCCA TGCTGAAGAG TATGGAGCTG GTGATCTCAT CCATAACAGA
    CTTCCAAGTG AATATCTTCA AGGCAAGGAT AGACAGAGAG CCCTGATTGC AGCAGTGCGA
    CATCACTTGA AGAAAGTTAA CTACCAAAAC TTTGACACTT TGCTGGCAGC CTTCAGGCAT
    TATGATAAGA ATGGAGATGG GGTGATAGAT AAAGCCGAGC TTCGGGAGGC TTGTGAACAG
    GTCAGCTTGC ACTTAGATGA GAAGCTCCTG GACCAGCTAT TTGACTACTG TGATGTGGAT
    AATGATGGGC TGATTAACTA CCTAGAATTT GCAAATTTTC TTAACTGGAA GGATAAAATG
    CCTCTTAAAG AGTATGAAGA GAGGGTCATA ATTAAAGGTA GAAAACCAGA TTGTACAAAC
    CGTACTGAGG CTAATGTGGA AGAATCTGAA CCAACTCTCC TAATAAAACC AGAAGATATA
    GTCTTAAAAG AACCAGGGAG CTCAGAAAAG ACTCTCCGGA CGCTTCTGAG GCCAAGTGAT
    AAAGTTTCTA ATTACTATAA GACAACTTCT TCTGAGATCA GTGCGGTTGT AGGAGCTATT
    CCTTCTACTT GTTATCCCAT CTATGGTGTT CCAACCATTC GGTCTGATAT TCCTGCCCCC
    CGAATTCGTC GCATCAGTGA TAGAACTAAT TATGGTGAAG AGGGCAATGC CTATTCACTA
    CTACATCCTA CTGTCTTTGG CCAGAAAGGA GTGTTTGAAA GAGACTTCTT CAAGACCAGA
    TCAAAAAAAG AGATTGCAGA GATATTATGT AACATTGGTG TTAAGCTTTC TGATGAAGAA
    TTTGAAAATG TATGGAATCT TGCATCAAAA AAACACCACA GAGGAGAAGT TTGTGTTGAA
    ACCATCAGAA ATGTTCTGGA TGAGCTACAA CATGCAGACA GAATCAAGTG TAAAACAAGC
    ATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe24088
    Clone ID Related Accession (Same CDS sequence) XM_005634369.3 , XM_005634369.2
    Accession Version XM_005634369.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2433bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product EF-hand domain-containing family member B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006605.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005634369.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGTGTTCTT TTGTGAGACT GGGGTCCCCT AAAGGGTTGA AGCACCACAA GATATTAGGA 
    ACTTCCCTTG AGCCAGTTAC AGCTTTTCCT TTAGAGATGA GTGACGTTAA AGGAATGAGG
    GTGTCAGAGA GGAAATCTTC TCGGAGCATG GAGGTTGGGC TCACCCACGA AGGAAAGGAT
    GACTTAGGAG AGACGGGGGT CCTTATGGGA GCAAGATCTC CCATAAGGGA GTTAGGGTTC
    AGAGTGGGAT TAGGAAAAGA AGATTCCTCA TGCAGGGAGA GCCTCCTGGT TGGTAACAAA
    TTTGAAGAAA GGATAACATC ACCAGAAACA AAATTTCCAT TGGGGATGGA GCTTGAAATG
    AAATTAAAAA GACAGAATCT TTCTGGAACC CTCCCGGAGA GGAGTAATTT AGGAGTCTAT
    GGGGTCTCAG CCTCCCAGGA AACAAAATCT TCTCCAGGTA TGTTGCCAGG AAGAAAGGGT
    GTAGAGAATG AGTATCTTAT GGCAGGATGT TCTGGAAGGA TAATACAGCC TCACTTGGGC
    ATGGTATGTG AAAATCCACA GACTCTAAGG AGCAGGAAAG GTTCTGTGGC TGGTTCCTCT
    GAAGGGTTAA GGGCTTCACT GGAGATGCAG CCTACTTTGG ATATGGAACC TAGACAGGAA
    CTGGAAAAAG AGACTTGTGT GGTGGTGAAG CCCAGTGCAG AGATGAAGCC TCCTGTGGAT
    GTGGAGGTGG ACACTGGTCT ACCCAAACTA GAAGAGCCAG ATGAAACAGG GCCCCAAATA
    GGTTTGGTGA TAGAACCTAC TGAGAGCCAG TTTGCCCAAC AACCTGAGGA GAGAAAGGAG
    GCTGAAAACA CTGAACCAGG AGTGGAGCCT CCAGATCGCA TCAGACCGGT ATACTCTGGG
    AAATTTTTTG ATCGGATGCC TTGCTGGCCA AGTGCAGGGA AAGTTATTCC TATTGGATAC
    AGAGTTGCAA CCTGTTTGAC TGAAAAACTT CCCAGGCTAA TTACTCCACC TGAGGCAAAA
    AAATTTTTCA ACTATAGAAA CCCACCAGCT GGAGCCGAAA GAGTATTTTA TGGCAGAGCA
    AATGATCCTC AGATTGCTCC TTATTTGACA CATGGAATAA GATCTAGAAT TTCAATACCG
    GCAAAGGTAC TGATAAACCC ACAGCCTGTA ACGACATTTC AACAGAAAAT TAAAGATAAA
    AAGGAATCAA TATATTTTAG TAATCAACGA GCACCACTAG GGAAATCTCA TGATCAAACG
    CCAGGATTAC CTAAAGGTCT GGATGTCCTC AATATGACAT TTGGGACAGC AGTCATCAGA
    GAATTATCTG CTAGAGATGT AGTGAATCCA CCAAAACCCT ATGAAGAAGT AGTTAAAGAA
    GGAAAAGAAG GACATGATCT GTATATTGTT TCTCACAATG ATTATTATGC AGGAGAAGCA
    AAGAACCGAA AGTATAACCC ATCAAGTTTC CATAGGTTTA ACTTGTATGG TGTCCCAACA
    CCACATTTTA ATGATGGAAG AGCCATGGCA AAAACTTTAT ATTGGCTCCA TGACCTACAA
    ATGAAAAGAG GAGCTAAGTT TGTATCCAAG AGAGTTGATG ATTTCAAAGA AAAGTTTCAA
    CATGAACTTG GAAGAGTTTT AGATCCCATT GCAGAAACAA TGAATATTCC CCCAGACCAC
    ACATTTGGAG CTTGTCTCCA TGCTGAAGAG TATGGAGCTG GTGATCTCAT CCATAACAGA
    CTTCCAAGTG AATATCTTCA AGGCAAGGAT AGACAGAGAG CCCTGATTGC AGCAGTGCGA
    CATCACTTGA AGAAAGTTAA CTACCAAAAC TTTGACACTT TGCTGGCAGC CTTCAGGCAT
    TATGATAAGA ATGGAGATGG GGTGATAGAT AAAGCCGAGC TTCGGGAGGC TTGTGAACAG
    GTCAGCTTGC ACTTAGATGA GAAGCTCCTG GACCAGCTAT TTGACTACTG TGATGTGGAT
    AATGATGGGC TGATTAACTA CCTAGAATTT GCAAATTTTC TTAACTGGAA GGATAAAATG
    CCTCTTAAAG AGTATGAAGA GAGGGTCATA ATTAAAGGTT ATCCCATCTA TGGTGTTCCA
    ACCATTCGGT CTGATATTCC TGCCCCCCGA ATTCGTCGCA TCAGTGATAG AACTAATTAT
    GGTGAAGAGG GCAATGCCTA TTCACTACTA CATCCTACTG TCTTTGGCCA GAAAGGAGTG
    TTTGAAAGAG ACTTCTTCAA GACCAGATCA AAAAAAGAGA TTGCAGAGAT ATTATGTAAC
    ATTGGTGTTA AGCTTTCTGA TGAAGAATTT GAAAATGTAT GGAATCTTGC ATCAAAAAAA
    CACCACAGAG GAGAAGTTTG TGTTGAAACC ATCAGAAATG TTCTGGATGA GCTACAACAT
    GCAGACAGAA TCAAGTGTAA AACAAGCATG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005634426.1
    CDS224..2656
    Translation

    Target ORF information:

    RefSeq Version XM_005634369.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris EF-hand domain family member B (EFHB), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005634369.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGTGTTCTT TTGTGAGACT GGGGTCCCCT AAAGGGTTGA AGCACCACAA GATATTAGGA 
    ACTTCCCTTG AGCCAGTTAC AGCTTTTCCT TTAGAGATGA GTGACGTTAA AGGAATGAGG
    GTGTCAGAGA GGAAATCTTC TCGGAGCATG GAGGTTGGGC TCACCCACGA AGGAAAGGAT
    GACTTAGGAG AGACGGGGGT CCTTATGGGA GCAAGATCTC CCATAAGGGA GTTAGGGTTC
    AGAGTGGGAT TAGGAAAAGA AGATTCCTCA TGCAGGGAGA GCCTCCTGGT TGGTAACAAA
    TTTGAAGAAA GGATAACATC ACCAGAAACA AAATTTCCAT TGGGGATGGA GCTTGAAATG
    AAATTAAAAA GACAGAATCT TTCTGGAACC CTCCCGGAGA GGAGTAATTT AGGAGTCTAT
    GGGGTCTCAG CCTCCCAGGA AACAAAATCT TCTCCAGGTA TGTTGCCAGG AAGAAAGGGT
    GTAGAGAATG AGTATCTTAT GGCAGGATGT TCTGGAAGGA TAATACAGCC TCACTTGGGC
    ATGGTATGTG AAAATCCACA GACTCTAAGG AGCAGGAAAG GTTCTGTGGC TGGTTCCTCT
    GAAGGGTTAA GGGCTTCACT GGAGATGCAG CCTACTTTGG ATATGGAACC TAGACAGGAA
    CTGGAAAAAG AGACTTGTGT GGTGGTGAAG CCCAGTGCAG AGATGAAGCC TCCTGTGGAT
    GTGGAGGTGG ACACTGGTCT ACCCAAACTA GAAGAGCCAG ATGAAACAGG GCCCCAAATA
    GGTTTGGTGA TAGAACCTAC TGAGAGCCAG TTTGCCCAAC AACCTGAGGA GAGAAAGGAG
    GCTGAAAACA CTGAACCAGG AGTGGAGCCT CCAGATCGCA TCAGACCGGT ATACTCTGGG
    AAATTTTTTG ATCGGATGCC TTGCTGGCCA AGTGCAGGGA AAGTTATTCC TATTGGATAC
    AGAGTTGCAA CCTGTTTGAC TGAAAAACTT CCCAGGCTAA TTACTCCACC TGAGGCAAAA
    AAATTTTTCA ACTATAGAAA CCCACCAGCT GGAGCCGAAA GAGTATTTTA TGGCAGAGCA
    AATGATCCTC AGATTGCTCC TTATTTGACA CATGGAATAA GATCTAGAAT TTCAATACCG
    GCAAAGGTAC TGATAAACCC ACAGCCTGTA ACGACATTTC AACAGAAAAT TAAAGATAAA
    AAGGAATCAA TATATTTTAG TAATCAACGA GCACCACTAG GGAAATCTCA TGATCAAACG
    CCAGGATTAC CTAAAGGTCT GGATGTCCTC AATATGACAT TTGGGACAGC AGTCATCAGA
    GAATTATCTG CTAGAGATGT AGTGAATCCA CCAAAACCCT ATGAAGAAGT AGTTAAAGAA
    GGAAAAGAAG GACATGATCT GTATATTGTT TCTCACAATG ATTATTATGC AGGAGAAGCA
    AAGAACCGAA AGTATAACCC ATCAAGTTTC CATAGGTTTA ACTTGTATGG TGTCCCAACA
    CCACATTTTA ATGATGGAAG AGCCATGGCA AAAACTTTAT ATTGGCTCCA TGACCTACAA
    ATGAAAAGAG GAGCTAAGTT TGTATCCAAG AGAGTTGATG ATTTCAAAGA AAAGTTTCAA
    CATGAACTTG GAAGAGTTTT AGATCCCATT GCAGAAACAA TGAATATTCC CCCAGACCAC
    ACATTTGGAG CTTGTCTCCA TGCTGAAGAG TATGGAGCTG GTGATCTCAT CCATAACAGA
    CTTCCAAGTG AATATCTTCA AGGCAAGGAT AGACAGAGAG CCCTGATTGC AGCAGTGCGA
    CATCACTTGA AGAAAGTTAA CTACCAAAAC TTTGACACTT TGCTGGCAGC CTTCAGGCAT
    TATGATAAGA ATGGAGATGG GGTGATAGAT AAAGCCGAGC TTCGGGAGGC TTGTGAACAG
    GTCAGCTTGC ACTTAGATGA GAAGCTCCTG GACCAGCTAT TTGACTACTG TGATGTGGAT
    AATGATGGGC TGATTAACTA CCTAGAATTT GCAAATTTTC TTAACTGGAA GGATAAAATG
    CCTCTTAAAG AGTATGAAGA GAGGGTCATA ATTAAAGGTT ATCCCATCTA TGGTGTTCCA
    ACCATTCGGT CTGATATTCC TGCCCCCCGA ATTCGTCGCA TCAGTGATAG AACTAATTAT
    GGTGAAGAGG GCAATGCCTA TTCACTACTA CATCCTACTG TCTTTGGCCA GAAAGGAGTG
    TTTGAAAGAG ACTTCTTCAA GACCAGATCA AAAAAAGAGA TTGCAGAGAT ATTATGTAAC
    ATTGGTGTTA AGCTTTCTGA TGAAGAATTT GAAAATGTAT GGAATCTTGC ATCAAAAAAA
    CACCACAGAG GAGAAGTTTG TGTTGAAACC ATCAGAAATG TTCTGGATGA GCTACAACAT
    GCAGACAGAA TCAAGTGTAA AACAAGCATG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe24088
    Clone ID Related Accession (Same CDS sequence) XM_005634369.3 , XM_005634369.2
    Accession Version XM_005634369.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2433bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product EF-hand domain-containing family member B isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726100.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005634369.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGTGTTCTT TTGTGAGACT GGGGTCCCCT AAAGGGTTGA AGCACCACAA GATATTAGGA 
    ACTTCCCTTG AGCCAGTTAC AGCTTTTCCT TTAGAGATGA GTGACGTTAA AGGAATGAGG
    GTGTCAGAGA GGAAATCTTC TCGGAGCATG GAGGTTGGGC TCACCCACGA AGGAAAGGAT
    GACTTAGGAG AGACGGGGGT CCTTATGGGA GCAAGATCTC CCATAAGGGA GTTAGGGTTC
    AGAGTGGGAT TAGGAAAAGA AGATTCCTCA TGCAGGGAGA GCCTCCTGGT TGGTAACAAA
    TTTGAAGAAA GGATAACATC ACCAGAAACA AAATTTCCAT TGGGGATGGA GCTTGAAATG
    AAATTAAAAA GACAGAATCT TTCTGGAACC CTCCCGGAGA GGAGTAATTT AGGAGTCTAT
    GGGGTCTCAG CCTCCCAGGA AACAAAATCT TCTCCAGGTA TGTTGCCAGG AAGAAAGGGT
    GTAGAGAATG AGTATCTTAT GGCAGGATGT TCTGGAAGGA TAATACAGCC TCACTTGGGC
    ATGGTATGTG AAAATCCACA GACTCTAAGG AGCAGGAAAG GTTCTGTGGC TGGTTCCTCT
    GAAGGGTTAA GGGCTTCACT GGAGATGCAG CCTACTTTGG ATATGGAACC TAGACAGGAA
    CTGGAAAAAG AGACTTGTGT GGTGGTGAAG CCCAGTGCAG AGATGAAGCC TCCTGTGGAT
    GTGGAGGTGG ACACTGGTCT ACCCAAACTA GAAGAGCCAG ATGAAACAGG GCCCCAAATA
    GGTTTGGTGA TAGAACCTAC TGAGAGCCAG TTTGCCCAAC AACCTGAGGA GAGAAAGGAG
    GCTGAAAACA CTGAACCAGG AGTGGAGCCT CCAGATCGCA TCAGACCGGT ATACTCTGGG
    AAATTTTTTG ATCGGATGCC TTGCTGGCCA AGTGCAGGGA AAGTTATTCC TATTGGATAC
    AGAGTTGCAA CCTGTTTGAC TGAAAAACTT CCCAGGCTAA TTACTCCACC TGAGGCAAAA
    AAATTTTTCA ACTATAGAAA CCCACCAGCT GGAGCCGAAA GAGTATTTTA TGGCAGAGCA
    AATGATCCTC AGATTGCTCC TTATTTGACA CATGGAATAA GATCTAGAAT TTCAATACCG
    GCAAAGGTAC TGATAAACCC ACAGCCTGTA ACGACATTTC AACAGAAAAT TAAAGATAAA
    AAGGAATCAA TATATTTTAG TAATCAACGA GCACCACTAG GGAAATCTCA TGATCAAACG
    CCAGGATTAC CTAAAGGTCT GGATGTCCTC AATATGACAT TTGGGACAGC AGTCATCAGA
    GAATTATCTG CTAGAGATGT AGTGAATCCA CCAAAACCCT ATGAAGAAGT AGTTAAAGAA
    GGAAAAGAAG GACATGATCT GTATATTGTT TCTCACAATG ATTATTATGC AGGAGAAGCA
    AAGAACCGAA AGTATAACCC ATCAAGTTTC CATAGGTTTA ACTTGTATGG TGTCCCAACA
    CCACATTTTA ATGATGGAAG AGCCATGGCA AAAACTTTAT ATTGGCTCCA TGACCTACAA
    ATGAAAAGAG GAGCTAAGTT TGTATCCAAG AGAGTTGATG ATTTCAAAGA AAAGTTTCAA
    CATGAACTTG GAAGAGTTTT AGATCCCATT GCAGAAACAA TGAATATTCC CCCAGACCAC
    ACATTTGGAG CTTGTCTCCA TGCTGAAGAG TATGGAGCTG GTGATCTCAT CCATAACAGA
    CTTCCAAGTG AATATCTTCA AGGCAAGGAT AGACAGAGAG CCCTGATTGC AGCAGTGCGA
    CATCACTTGA AGAAAGTTAA CTACCAAAAC TTTGACACTT TGCTGGCAGC CTTCAGGCAT
    TATGATAAGA ATGGAGATGG GGTGATAGAT AAAGCCGAGC TTCGGGAGGC TTGTGAACAG
    GTCAGCTTGC ACTTAGATGA GAAGCTCCTG GACCAGCTAT TTGACTACTG TGATGTGGAT
    AATGATGGGC TGATTAACTA CCTAGAATTT GCAAATTTTC TTAACTGGAA GGATAAAATG
    CCTCTTAAAG AGTATGAAGA GAGGGTCATA ATTAAAGGTT ATCCCATCTA TGGTGTTCCA
    ACCATTCGGT CTGATATTCC TGCCCCCCGA ATTCGTCGCA TCAGTGATAG AACTAATTAT
    GGTGAAGAGG GCAATGCCTA TTCACTACTA CATCCTACTG TCTTTGGCCA GAAAGGAGTG
    TTTGAAAGAG ACTTCTTCAA GACCAGATCA AAAAAAGAGA TTGCAGAGAT ATTATGTAAC
    ATTGGTGTTA AGCTTTCTGA TGAAGAATTT GAAAATGTAT GGAATCTTGC ATCAAAAAAA
    CACCACAGAG GAGAAGTTTG TGTTGAAACC ATCAGAAATG TTCTGGATGA GCTACAACAT
    GCAGACAGAA TCAAGTGTAA AACAAGCATG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005634426.1
    CDS79..2511
    Translation

    Target ORF information:

    RefSeq Version XM_005634369.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris EF-hand domain family, member B (EFHB), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005634369.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGTGTTCTT TTGTGAGACT GGGGTCCCCT AAAGGGTTGA AGCACCACAA GATATTAGGA 
    ACTTCCCTTG AGCCAGTTAC AGCTTTTCCT TTAGAGATGA GTGACGTTAA AGGAATGAGG
    GTGTCAGAGA GGAAATCTTC TCGGAGCATG GAGGTTGGGC TCACCCACGA AGGAAAGGAT
    GACTTAGGAG AGACGGGGGT CCTTATGGGA GCAAGATCTC CCATAAGGGA GTTAGGGTTC
    AGAGTGGGAT TAGGAAAAGA AGATTCCTCA TGCAGGGAGA GCCTCCTGGT TGGTAACAAA
    TTTGAAGAAA GGATAACATC ACCAGAAACA AAATTTCCAT TGGGGATGGA GCTTGAAATG
    AAATTAAAAA GACAGAATCT TTCTGGAACC CTCCCGGAGA GGAGTAATTT AGGAGTCTAT
    GGGGTCTCAG CCTCCCAGGA AACAAAATCT TCTCCAGGTA TGTTGCCAGG AAGAAAGGGT
    GTAGAGAATG AGTATCTTAT GGCAGGATGT TCTGGAAGGA TAATACAGCC TCACTTGGGC
    ATGGTATGTG AAAATCCACA GACTCTAAGG AGCAGGAAAG GTTCTGTGGC TGGTTCCTCT
    GAAGGGTTAA GGGCTTCACT GGAGATGCAG CCTACTTTGG ATATGGAACC TAGACAGGAA
    CTGGAAAAAG AGACTTGTGT GGTGGTGAAG CCCAGTGCAG AGATGAAGCC TCCTGTGGAT
    GTGGAGGTGG ACACTGGTCT ACCCAAACTA GAAGAGCCAG ATGAAACAGG GCCCCAAATA
    GGTTTGGTGA TAGAACCTAC TGAGAGCCAG TTTGCCCAAC AACCTGAGGA GAGAAAGGAG
    GCTGAAAACA CTGAACCAGG AGTGGAGCCT CCAGATCGCA TCAGACCGGT ATACTCTGGG
    AAATTTTTTG ATCGGATGCC TTGCTGGCCA AGTGCAGGGA AAGTTATTCC TATTGGATAC
    AGAGTTGCAA CCTGTTTGAC TGAAAAACTT CCCAGGCTAA TTACTCCACC TGAGGCAAAA
    AAATTTTTCA ACTATAGAAA CCCACCAGCT GGAGCCGAAA GAGTATTTTA TGGCAGAGCA
    AATGATCCTC AGATTGCTCC TTATTTGACA CATGGAATAA GATCTAGAAT TTCAATACCG
    GCAAAGGTAC TGATAAACCC ACAGCCTGTA ACGACATTTC AACAGAAAAT TAAAGATAAA
    AAGGAATCAA TATATTTTAG TAATCAACGA GCACCACTAG GGAAATCTCA TGATCAAACG
    CCAGGATTAC CTAAAGGTCT GGATGTCCTC AATATGACAT TTGGGACAGC AGTCATCAGA
    GAATTATCTG CTAGAGATGT AGTGAATCCA CCAAAACCCT ATGAAGAAGT AGTTAAAGAA
    GGAAAAGAAG GACATGATCT GTATATTGTT TCTCACAATG ATTATTATGC AGGAGAAGCA
    AAGAACCGAA AGTATAACCC ATCAAGTTTC CATAGGTTTA ACTTGTATGG TGTCCCAACA
    CCACATTTTA ATGATGGAAG AGCCATGGCA AAAACTTTAT ATTGGCTCCA TGACCTACAA
    ATGAAAAGAG GAGCTAAGTT TGTATCCAAG AGAGTTGATG ATTTCAAAGA AAAGTTTCAA
    CATGAACTTG GAAGAGTTTT AGATCCCATT GCAGAAACAA TGAATATTCC CCCAGACCAC
    ACATTTGGAG CTTGTCTCCA TGCTGAAGAG TATGGAGCTG GTGATCTCAT CCATAACAGA
    CTTCCAAGTG AATATCTTCA AGGCAAGGAT AGACAGAGAG CCCTGATTGC AGCAGTGCGA
    CATCACTTGA AGAAAGTTAA CTACCAAAAC TTTGACACTT TGCTGGCAGC CTTCAGGCAT
    TATGATAAGA ATGGAGATGG GGTGATAGAT AAAGCCGAGC TTCGGGAGGC TTGTGAACAG
    GTCAGCTTGC ACTTAGATGA GAAGCTCCTG GACCAGCTAT TTGACTACTG TGATGTGGAT
    AATGATGGGC TGATTAACTA CCTAGAATTT GCAAATTTTC TTAACTGGAA GGATAAAATG
    CCTCTTAAAG AGTATGAAGA GAGGGTCATA ATTAAAGGTT ATCCCATCTA TGGTGTTCCA
    ACCATTCGGT CTGATATTCC TGCCCCCCGA ATTCGTCGCA TCAGTGATAG AACTAATTAT
    GGTGAAGAGG GCAATGCCTA TTCACTACTA CATCCTACTG TCTTTGGCCA GAAAGGAGTG
    TTTGAAAGAG ACTTCTTCAA GACCAGATCA AAAAAAGAGA TTGCAGAGAT ATTATGTAAC
    ATTGGTGTTA AGCTTTCTGA TGAAGAATTT GAAAATGTAT GGAATCTTGC ATCAAAAAAA
    CACCACAGAG GAGAAGTTTG TGTTGAAACC ATCAGAAATG TTCTGGATGA GCTACAACAT
    GCAGACAGAA TCAAGTGTAA AACAAGCATG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe24089
    Clone ID Related Accession (Same CDS sequence) XM_005634370.1 , XM_005634370.2
    Accession Version XM_005634370.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2148bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product EF-hand domain-containing family member B isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726100.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTGTTCTT TTGTGAGACT GGGGTCCCCT AAAGGGTTGA AGCACCACAA GATATTAGGA 
    ACTTCCCTTG AGCCAGTTAC AGCTTTTCCT TTAGAGATGA GTGACGTTAA AGGAATGAGG
    GTGTCAGAGA GGAAATCTTC TCGGAGCATG GAGGTTGGGC TCACCCACGA AGGAAAGGAT
    GACTTAGGAG AGACGGGGGT CCTTATGGGA GCAAGATCTC CCATAAGGGA GTTAGGGTTC
    AGAGTGGGAT TAGGAAAAGA AGATTCCTCA TGCAGGGAGA GCCTCCTGGT TGGTAACAAA
    TTTGAAGAAA GGATAACATC ACCAGAAACA AAATTTCCAT TGGGGATGGA GCTTGAAATG
    AAATTAAAAA GACAGAATCT TTCTGGAACC CTCCCGGAGA GGAGTAATTT AGGAGTCTAT
    GGGGTCTCAG CCTCCCAGGA AACAAAATCT TCTCCAGGTA TGTTGCCAGG AAGAAAGGGT
    GTAGAGAATG AGTATCTTAT GGCAGGATGT TCTGGAAGGA TAATACAGCC TCACTTGGGC
    ATGGTATGTG AAAATCCACA GACTCTAAGG AGCAGGAAAG GTTCTGTGGC TGGTTCCTCT
    GAAGGGTTAA GGGCTTCACT GGAGATGCAG CCTACTTTGG ATATGGAACC TAGACAGGAA
    CTGGAAAAAG AGACTTGTGT GGTGGTGAAG CCCAGTGCAG AGATGAAGCC TCCTGTGGAT
    GTGGAGGTGG ACACTGGTCT ACCCAAACTA GAAGAGCCAG ATGAAACAGG GCCCCAAATA
    GGTTTGGTGA TAGAACCTAC TGAGAGCCAG TTTGCCCAAC AACCTGAGGA GAGAAAGGAG
    GCTGAAAACA CTGAACCAGG AGTGGAGCCT CCAGATCGCA TCAGACCGGT ATACTCTGGG
    AAATTTTTTG ATCGGATGCC TTGCTGGCCA AGTGCAGGGA AAGTTATTCC TATTGGATAC
    AGAGTTGCAA CCTGTTTGAC TGAAAAACTT CCCAGGCTAA TTACTCCACC TGAGGCAAAA
    AAATTTTTCA ACTATAGAAA CCCACCAGCT GGAGCCGAAA GAGTATTTTA TGGCAGAGCA
    AATGATCCTC AGATTGCTCC TTATTTGACA CATGGAATAA GATCTAGAAT TTCAATACCG
    GCAAAGGTAC TGATAAACCC ACAGCCTGTA ACGACATTTC AACAGAAAAT TAAAGATAAA
    AAGGAATCAA TATATTTTAG TAATCAACGA GCACCACTAG GGAAATCTCA TGATCAAACG
    CCAGGATTAC CTAAAGGTCT GGATGTCCTC AATATGACAT TTGGGACAGC AGTCATCAGA
    GAATTATCTG CTAGAGATGT AGTGAATCCA CCAAAACCCT ATGAAGAAGT AGTTAAAGAA
    GGAAAAGAAG GACATGATCT GTATATTGTT TCTCACAATG ATTATTATGC AGGAGAAGCA
    AAGAACCGAA AGTATAACCC ATCAAGTTTC CATAGGTTTA ACTTGTATGG TGTCCCAACA
    CCACATTTTA ATGATGGAAG AGCCATGGCA AAAACTTTAT ATTGGCTCCA TGACCTACAA
    ATGAAAAGAG GAGCTAAGTT TGTATCCAAG AGAGTTGATG ATTTCAAAGA AAAGTTTCAA
    CATGAACTTG GAAGAGTTTT AGATCCCATT GCAGAAACAA TGAATATTCC CCCAGACCAC
    ACATTTGGAG CTTGTCTCCA TGCTGAAGAG TATGGAGCTG GTGATCTCAT CCATAACAGA
    CTTCCAAGTG AATATCTTCA AGGCAAGGAT AGACAGAGAG CCCTGATTGC AGCAGTGCGA
    CATCACTTGA AGAAAGTTAA CTACCAAAAC TTTGACACTT TGCTGGCAGC CTTCAGGCAT
    TATGATAAGA ATGGAGATGG GGTGATAGAT AAAGCCGAGC TTCGGGAGGC TTGTGAACAG
    GTCAGCTTGC ACTTAGATGA GAAGCTCCTG GACCAGCTAT TTGACTACTG TGATGTGGAT
    AATGATGGGC TGATTAACTA CCTAGAATTT GCAAATTTTC TTAACTGGAA GGATAAAATG
    CCTCTTAAAG AGTATGAAGA GAGGGTCATA ATTAAAGTGG GGTGCTGGCA TCGGTGGAGG
    GCTTTCACCA GCCCAGTCTC AGTCTATACC AGCAAGGAGA GAGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005634427.1
    CDS79..2226
    Translation

    Target ORF information:

    RefSeq Version XM_005634370.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris EF-hand domain family, member B (EFHB), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005634370.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTGTTCTT TTGTGAGACT GGGGTCCCCT AAAGGGTTGA AGCACCACAA GATATTAGGA 
    ACTTCCCTTG AGCCAGTTAC AGCTTTTCCT TTAGAGATGA GTGACGTTAA AGGAATGAGG
    GTGTCAGAGA GGAAATCTTC TCGGAGCATG GAGGTTGGGC TCACCCACGA AGGAAAGGAT
    GACTTAGGAG AGACGGGGGT CCTTATGGGA GCAAGATCTC CCATAAGGGA GTTAGGGTTC
    AGAGTGGGAT TAGGAAAAGA AGATTCCTCA TGCAGGGAGA GCCTCCTGGT TGGTAACAAA
    TTTGAAGAAA GGATAACATC ACCAGAAACA AAATTTCCAT TGGGGATGGA GCTTGAAATG
    AAATTAAAAA GACAGAATCT TTCTGGAACC CTCCCGGAGA GGAGTAATTT AGGAGTCTAT
    GGGGTCTCAG CCTCCCAGGA AACAAAATCT TCTCCAGGTA TGTTGCCAGG AAGAAAGGGT
    GTAGAGAATG AGTATCTTAT GGCAGGATGT TCTGGAAGGA TAATACAGCC TCACTTGGGC
    ATGGTATGTG AAAATCCACA GACTCTAAGG AGCAGGAAAG GTTCTGTGGC TGGTTCCTCT
    GAAGGGTTAA GGGCTTCACT GGAGATGCAG CCTACTTTGG ATATGGAACC TAGACAGGAA
    CTGGAAAAAG AGACTTGTGT GGTGGTGAAG CCCAGTGCAG AGATGAAGCC TCCTGTGGAT
    GTGGAGGTGG ACACTGGTCT ACCCAAACTA GAAGAGCCAG ATGAAACAGG GCCCCAAATA
    GGTTTGGTGA TAGAACCTAC TGAGAGCCAG TTTGCCCAAC AACCTGAGGA GAGAAAGGAG
    GCTGAAAACA CTGAACCAGG AGTGGAGCCT CCAGATCGCA TCAGACCGGT ATACTCTGGG
    AAATTTTTTG ATCGGATGCC TTGCTGGCCA AGTGCAGGGA AAGTTATTCC TATTGGATAC
    AGAGTTGCAA CCTGTTTGAC TGAAAAACTT CCCAGGCTAA TTACTCCACC TGAGGCAAAA
    AAATTTTTCA ACTATAGAAA CCCACCAGCT GGAGCCGAAA GAGTATTTTA TGGCAGAGCA
    AATGATCCTC AGATTGCTCC TTATTTGACA CATGGAATAA GATCTAGAAT TTCAATACCG
    GCAAAGGTAC TGATAAACCC ACAGCCTGTA ACGACATTTC AACAGAAAAT TAAAGATAAA
    AAGGAATCAA TATATTTTAG TAATCAACGA GCACCACTAG GGAAATCTCA TGATCAAACG
    CCAGGATTAC CTAAAGGTCT GGATGTCCTC AATATGACAT TTGGGACAGC AGTCATCAGA
    GAATTATCTG CTAGAGATGT AGTGAATCCA CCAAAACCCT ATGAAGAAGT AGTTAAAGAA
    GGAAAAGAAG GACATGATCT GTATATTGTT TCTCACAATG ATTATTATGC AGGAGAAGCA
    AAGAACCGAA AGTATAACCC ATCAAGTTTC CATAGGTTTA ACTTGTATGG TGTCCCAACA
    CCACATTTTA ATGATGGAAG AGCCATGGCA AAAACTTTAT ATTGGCTCCA TGACCTACAA
    ATGAAAAGAG GAGCTAAGTT TGTATCCAAG AGAGTTGATG ATTTCAAAGA AAAGTTTCAA
    CATGAACTTG GAAGAGTTTT AGATCCCATT GCAGAAACAA TGAATATTCC CCCAGACCAC
    ACATTTGGAG CTTGTCTCCA TGCTGAAGAG TATGGAGCTG GTGATCTCAT CCATAACAGA
    CTTCCAAGTG AATATCTTCA AGGCAAGGAT AGACAGAGAG CCCTGATTGC AGCAGTGCGA
    CATCACTTGA AGAAAGTTAA CTACCAAAAC TTTGACACTT TGCTGGCAGC CTTCAGGCAT
    TATGATAAGA ATGGAGATGG GGTGATAGAT AAAGCCGAGC TTCGGGAGGC TTGTGAACAG
    GTCAGCTTGC ACTTAGATGA GAAGCTCCTG GACCAGCTAT TTGACTACTG TGATGTGGAT
    AATGATGGGC TGATTAACTA CCTAGAATTT GCAAATTTTC TTAACTGGAA GGATAAAATG
    CCTCTTAAAG AGTATGAAGA GAGGGTCATA ATTAAAGTGG GGTGCTGGCA TCGGTGGAGG
    GCTTTCACCA GCCCAGTCTC AGTCTATACC AGCAAGGAGA GAGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe24089
    Clone ID Related Accession (Same CDS sequence) XM_005634370.1 , XM_005634370.2
    Accession Version XM_005634370.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2148bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product EF-hand domain-containing family member B isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006605.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005634370.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTGTTCTT TTGTGAGACT GGGGTCCCCT AAAGGGTTGA AGCACCACAA GATATTAGGA 
    ACTTCCCTTG AGCCAGTTAC AGCTTTTCCT TTAGAGATGA GTGACGTTAA AGGAATGAGG
    GTGTCAGAGA GGAAATCTTC TCGGAGCATG GAGGTTGGGC TCACCCACGA AGGAAAGGAT
    GACTTAGGAG AGACGGGGGT CCTTATGGGA GCAAGATCTC CCATAAGGGA GTTAGGGTTC
    AGAGTGGGAT TAGGAAAAGA AGATTCCTCA TGCAGGGAGA GCCTCCTGGT TGGTAACAAA
    TTTGAAGAAA GGATAACATC ACCAGAAACA AAATTTCCAT TGGGGATGGA GCTTGAAATG
    AAATTAAAAA GACAGAATCT TTCTGGAACC CTCCCGGAGA GGAGTAATTT AGGAGTCTAT
    GGGGTCTCAG CCTCCCAGGA AACAAAATCT TCTCCAGGTA TGTTGCCAGG AAGAAAGGGT
    GTAGAGAATG AGTATCTTAT GGCAGGATGT TCTGGAAGGA TAATACAGCC TCACTTGGGC
    ATGGTATGTG AAAATCCACA GACTCTAAGG AGCAGGAAAG GTTCTGTGGC TGGTTCCTCT
    GAAGGGTTAA GGGCTTCACT GGAGATGCAG CCTACTTTGG ATATGGAACC TAGACAGGAA
    CTGGAAAAAG AGACTTGTGT GGTGGTGAAG CCCAGTGCAG AGATGAAGCC TCCTGTGGAT
    GTGGAGGTGG ACACTGGTCT ACCCAAACTA GAAGAGCCAG ATGAAACAGG GCCCCAAATA
    GGTTTGGTGA TAGAACCTAC TGAGAGCCAG TTTGCCCAAC AACCTGAGGA GAGAAAGGAG
    GCTGAAAACA CTGAACCAGG AGTGGAGCCT CCAGATCGCA TCAGACCGGT ATACTCTGGG
    AAATTTTTTG ATCGGATGCC TTGCTGGCCA AGTGCAGGGA AAGTTATTCC TATTGGATAC
    AGAGTTGCAA CCTGTTTGAC TGAAAAACTT CCCAGGCTAA TTACTCCACC TGAGGCAAAA
    AAATTTTTCA ACTATAGAAA CCCACCAGCT GGAGCCGAAA GAGTATTTTA TGGCAGAGCA
    AATGATCCTC AGATTGCTCC TTATTTGACA CATGGAATAA GATCTAGAAT TTCAATACCG
    GCAAAGGTAC TGATAAACCC ACAGCCTGTA ACGACATTTC AACAGAAAAT TAAAGATAAA
    AAGGAATCAA TATATTTTAG TAATCAACGA GCACCACTAG GGAAATCTCA TGATCAAACG
    CCAGGATTAC CTAAAGGTCT GGATGTCCTC AATATGACAT TTGGGACAGC AGTCATCAGA
    GAATTATCTG CTAGAGATGT AGTGAATCCA CCAAAACCCT ATGAAGAAGT AGTTAAAGAA
    GGAAAAGAAG GACATGATCT GTATATTGTT TCTCACAATG ATTATTATGC AGGAGAAGCA
    AAGAACCGAA AGTATAACCC ATCAAGTTTC CATAGGTTTA ACTTGTATGG TGTCCCAACA
    CCACATTTTA ATGATGGAAG AGCCATGGCA AAAACTTTAT ATTGGCTCCA TGACCTACAA
    ATGAAAAGAG GAGCTAAGTT TGTATCCAAG AGAGTTGATG ATTTCAAAGA AAAGTTTCAA
    CATGAACTTG GAAGAGTTTT AGATCCCATT GCAGAAACAA TGAATATTCC CCCAGACCAC
    ACATTTGGAG CTTGTCTCCA TGCTGAAGAG TATGGAGCTG GTGATCTCAT CCATAACAGA
    CTTCCAAGTG AATATCTTCA AGGCAAGGAT AGACAGAGAG CCCTGATTGC AGCAGTGCGA
    CATCACTTGA AGAAAGTTAA CTACCAAAAC TTTGACACTT TGCTGGCAGC CTTCAGGCAT
    TATGATAAGA ATGGAGATGG GGTGATAGAT AAAGCCGAGC TTCGGGAGGC TTGTGAACAG
    GTCAGCTTGC ACTTAGATGA GAAGCTCCTG GACCAGCTAT TTGACTACTG TGATGTGGAT
    AATGATGGGC TGATTAACTA CCTAGAATTT GCAAATTTTC TTAACTGGAA GGATAAAATG
    CCTCTTAAAG AGTATGAAGA GAGGGTCATA ATTAAAGTGG GGTGCTGGCA TCGGTGGAGG
    GCTTTCACCA GCCCAGTCTC AGTCTATACC AGCAAGGAGA GAGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005634427.1
    CDS230..2377
    Translation

    Target ORF information:

    RefSeq Version XM_005634370.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris EF-hand domain family member B (EFHB), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005634370.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTGTTCTT TTGTGAGACT GGGGTCCCCT AAAGGGTTGA AGCACCACAA GATATTAGGA 
    ACTTCCCTTG AGCCAGTTAC AGCTTTTCCT TTAGAGATGA GTGACGTTAA AGGAATGAGG
    GTGTCAGAGA GGAAATCTTC TCGGAGCATG GAGGTTGGGC TCACCCACGA AGGAAAGGAT
    GACTTAGGAG AGACGGGGGT CCTTATGGGA GCAAGATCTC CCATAAGGGA GTTAGGGTTC
    AGAGTGGGAT TAGGAAAAGA AGATTCCTCA TGCAGGGAGA GCCTCCTGGT TGGTAACAAA
    TTTGAAGAAA GGATAACATC ACCAGAAACA AAATTTCCAT TGGGGATGGA GCTTGAAATG
    AAATTAAAAA GACAGAATCT TTCTGGAACC CTCCCGGAGA GGAGTAATTT AGGAGTCTAT
    GGGGTCTCAG CCTCCCAGGA AACAAAATCT TCTCCAGGTA TGTTGCCAGG AAGAAAGGGT
    GTAGAGAATG AGTATCTTAT GGCAGGATGT TCTGGAAGGA TAATACAGCC TCACTTGGGC
    ATGGTATGTG AAAATCCACA GACTCTAAGG AGCAGGAAAG GTTCTGTGGC TGGTTCCTCT
    GAAGGGTTAA GGGCTTCACT GGAGATGCAG CCTACTTTGG ATATGGAACC TAGACAGGAA
    CTGGAAAAAG AGACTTGTGT GGTGGTGAAG CCCAGTGCAG AGATGAAGCC TCCTGTGGAT
    GTGGAGGTGG ACACTGGTCT ACCCAAACTA GAAGAGCCAG ATGAAACAGG GCCCCAAATA
    GGTTTGGTGA TAGAACCTAC TGAGAGCCAG TTTGCCCAAC AACCTGAGGA GAGAAAGGAG
    GCTGAAAACA CTGAACCAGG AGTGGAGCCT CCAGATCGCA TCAGACCGGT ATACTCTGGG
    AAATTTTTTG ATCGGATGCC TTGCTGGCCA AGTGCAGGGA AAGTTATTCC TATTGGATAC
    AGAGTTGCAA CCTGTTTGAC TGAAAAACTT CCCAGGCTAA TTACTCCACC TGAGGCAAAA
    AAATTTTTCA ACTATAGAAA CCCACCAGCT GGAGCCGAAA GAGTATTTTA TGGCAGAGCA
    AATGATCCTC AGATTGCTCC TTATTTGACA CATGGAATAA GATCTAGAAT TTCAATACCG
    GCAAAGGTAC TGATAAACCC ACAGCCTGTA ACGACATTTC AACAGAAAAT TAAAGATAAA
    AAGGAATCAA TATATTTTAG TAATCAACGA GCACCACTAG GGAAATCTCA TGATCAAACG
    CCAGGATTAC CTAAAGGTCT GGATGTCCTC AATATGACAT TTGGGACAGC AGTCATCAGA
    GAATTATCTG CTAGAGATGT AGTGAATCCA CCAAAACCCT ATGAAGAAGT AGTTAAAGAA
    GGAAAAGAAG GACATGATCT GTATATTGTT TCTCACAATG ATTATTATGC AGGAGAAGCA
    AAGAACCGAA AGTATAACCC ATCAAGTTTC CATAGGTTTA ACTTGTATGG TGTCCCAACA
    CCACATTTTA ATGATGGAAG AGCCATGGCA AAAACTTTAT ATTGGCTCCA TGACCTACAA
    ATGAAAAGAG GAGCTAAGTT TGTATCCAAG AGAGTTGATG ATTTCAAAGA AAAGTTTCAA
    CATGAACTTG GAAGAGTTTT AGATCCCATT GCAGAAACAA TGAATATTCC CCCAGACCAC
    ACATTTGGAG CTTGTCTCCA TGCTGAAGAG TATGGAGCTG GTGATCTCAT CCATAACAGA
    CTTCCAAGTG AATATCTTCA AGGCAAGGAT AGACAGAGAG CCCTGATTGC AGCAGTGCGA
    CATCACTTGA AGAAAGTTAA CTACCAAAAC TTTGACACTT TGCTGGCAGC CTTCAGGCAT
    TATGATAAGA ATGGAGATGG GGTGATAGAT AAAGCCGAGC TTCGGGAGGC TTGTGAACAG
    GTCAGCTTGC ACTTAGATGA GAAGCTCCTG GACCAGCTAT TTGACTACTG TGATGTGGAT
    AATGATGGGC TGATTAACTA CCTAGAATTT GCAAATTTTC TTAACTGGAA GGATAAAATG
    CCTCTTAAAG AGTATGAAGA GAGGGTCATA ATTAAAGTGG GGTGCTGGCA TCGGTGGAGG
    GCTTTCACCA GCCCAGTCTC AGTCTATACC AGCAAGGAGA GAGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe42486
    Clone ID Related Accession (Same CDS sequence) XM_014106569.1 , XM_014106569.2
    Accession Version XM_014106569.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2541bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product EF-hand domain-containing family member B isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726100.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGTGTTCTT TTGTGAGACT GGGGTCCCCT AAAGGGTTGA AGCACCACAA GATATTAGGA 
    ACTTCCCTTG AGCCAGTTAC AGCTTTTCCT TTAGAGATGA GTGACGTTAA AGGAATGAGG
    GTGTCAGAGA GGAAATCTTC TCGGAGCATG GAGGTTGGGC TCACCCACGA AGGAAAGGAT
    GACTTAGGAG AGACGGGGGT CCTTATGGGA GCAAGATCTC CCATAAGGGA GTTAGGGTTC
    AGAGTGGGAT TAGGAAAAGA AGATTCCTCA TGCAGGGAGA GCCTCCTGGT TGGTAACAAA
    TTTGAAGAAA GGATAACATC ACCAGAAACA AAATTTCCAT TGGGGATGGA GCTTGAAATG
    AAATTAAAAA GACAGAATCT TTCTGGAACC CTCCCGGAGA GGAGTAATTT AGGAGTCTAT
    GGGGTCTCAG CCTCCCAGGA AACAAAATCT TCTCCAGGTA TGTTGCCAGG AAGAAAGGGT
    GTAGAGAATG AGTATCTTAT GGCAGGATGT TCTGGAAGGA TAATACAGCC TCACTTGGGC
    ATGGTATGTG AAAATCCACA GACTCTAAGG AGCAGGAAAG GTTCTGTGGC TGGTTCCTCT
    GAAGGGTTAA GGGCTTCACT GGAGATGCAG CCTACTTTGG ATATGGAACC TAGACAGGAA
    CTGGAAAAAG AGACTTGTGT GGTGGTGAAG CCCAGTGCAG AGATGAAGCC TCCTGTGGAT
    GTGGAGGTGG ACACTGGTCT ACCCAAACTA GAAGAGCCAG ATGAAACAGG GCCCCAAATA
    GGTTTGGTGA TAGAACCTAC TGAGAGCCAG TTTGCCCAAC AACCTGAGGA GAGAAAGGAG
    GCTGAAAACA CTGAACCAGG AGTGGAGCCT CCAGATCGCA TCAGACCGGT ATACTCTGGG
    AAATTTTTTG ATCGGATGCC TTGCTGGCCA AGTGCAGGGA AAGTTATTCC TATTGGATAC
    AGAGTTGCAA CCTGTTTGAC TGAAAAACTT CCCAGGCTAA TTACTCCACC TGAGGCAAAA
    AAATTTTTCA ACTATAGAAA CCCACCAGCT GGAGCCGAAA GAGTATTTTA TGGCAGAGCA
    AATGATCCTC AGATTGCTCC TTATTTGACA CATGGAATAA GATCTAGAAT TTCAATACCG
    GCAAAGGTAC TGATAAACCC ACAGCCTGTA ACGACATTTC AACAGAAAAT TAAAGATAAA
    AAGGAATCAA TATATTTTAG TAATCAACGA GCACCACTAG GGAAATCTCA TGATCAAACG
    CCAGGATTAC CTAAAGGTCT GGATGTCCTC AATATGACAT TTGGGACAGC AGTCATCAGA
    GAATTATCTG CTAGAGATGT AGTGAATCCA CCAAAACCCT ATGAAGAAGT AGTTAAAGAA
    GGAAAAGAAG GACATGATCT GTATATTGTT TCTCACAATG ATTATTATGC AGGGGACAAT
    AATATTGTGA GGATTAGGAA AAGAGGAGCT AAGTTTGTAT CCAAGAGAGT TGATGATTTC
    AAAGAAAAGT TTCAACATGA ACTTGGAAGA GTTTTAGATC CCATTGCAGA AACAATGAAT
    ATTCCCCCAG ACCACACATT TGGAGCTTGT CTCCATGCTG AAGAGTATGG AGCTGGTGAT
    CTCATCCATA ACAGACTTCC AAGTGAATAT CTTCAAGGCA AGGATAGACA GAGAGCCCTG
    ATTGCAGCAG TGCGACATCA CTTGAAGAAA GTTAACTACC AAAACTTTGA CACTTTGCTG
    GCAGCCTTCA GGCATTATGA TAAGAATGGA GATGGGGTGA TAGATAAAGC CGAGCTTCGG
    GAGGCTTGTG AACAGGTCAG CTTGCACTTA GATGAGAAGC TCCTGGACCA GCTATTTGAC
    TACTGTGATG TGGATAATGA TGGGCTGATT AACTACCTAG AATTTGCAAA TTTTCTTAAC
    TGGAAGGATA AAATGCCTCT TAAAGAGTAT GAAGAGAGGG TCATAATTAA AGGTAGAAAA
    CCAGATTGTA CAAACCGTAC TGAGGCTAAT GTGGAAGAAT CTGAACCAAC TCTCCTAATA
    AAACCAGAAG ATATAGTCTT AAAAGAACCA GGGAGCTCAG AAAAGACTCT CCGGACGCTT
    CTGAGGCCAA GTGATAAAGT TTCTAATTAC TATAAGACAA CTTCTTCTGA GATCAGTGCG
    GTTGTAGGAG CTATTCCTTC TACTTGTTAT CCCATCTATG GTGTTCCAAC CATTCGGTCT
    GATATTCCTG CCCCCCGAAT TCGTCGCATC AGTGATAGAA CTAATTATGG TGAAGAGGGC
    AATGCCTATT CACTACTACA TCCTACTGTC TTTGGCCAGA AAGGAGTGTT TGAAAGAGAC
    TTCTTCAAGA CCAGATCAAA AAAAGAGATT GCAGAGATAT TATGTAACAT TGGTGTTAAG
    CTTTCTGATG AAGAATTTGA AAATGTATGG AATCTTGCAT CAAAAAAACA CCACAGAGGA
    GAAGTTTGTG TTGAAACCAT CAGAAATGTT CTGGATGAGC TACAACATGC AGACAGAATC
    AAGTGTAAAA CAAGCATGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013962044.1
    CDS79..2619
    Translation

    Target ORF information:

    RefSeq Version XM_014106569.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris EF-hand domain family, member B (EFHB), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014106569.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGTGTTCTT TTGTGAGACT GGGGTCCCCT AAAGGGTTGA AGCACCACAA GATATTAGGA 
    ACTTCCCTTG AGCCAGTTAC AGCTTTTCCT TTAGAGATGA GTGACGTTAA AGGAATGAGG
    GTGTCAGAGA GGAAATCTTC TCGGAGCATG GAGGTTGGGC TCACCCACGA AGGAAAGGAT
    GACTTAGGAG AGACGGGGGT CCTTATGGGA GCAAGATCTC CCATAAGGGA GTTAGGGTTC
    AGAGTGGGAT TAGGAAAAGA AGATTCCTCA TGCAGGGAGA GCCTCCTGGT TGGTAACAAA
    TTTGAAGAAA GGATAACATC ACCAGAAACA AAATTTCCAT TGGGGATGGA GCTTGAAATG
    AAATTAAAAA GACAGAATCT TTCTGGAACC CTCCCGGAGA GGAGTAATTT AGGAGTCTAT
    GGGGTCTCAG CCTCCCAGGA AACAAAATCT TCTCCAGGTA TGTTGCCAGG AAGAAAGGGT
    GTAGAGAATG AGTATCTTAT GGCAGGATGT TCTGGAAGGA TAATACAGCC TCACTTGGGC
    ATGGTATGTG AAAATCCACA GACTCTAAGG AGCAGGAAAG GTTCTGTGGC TGGTTCCTCT
    GAAGGGTTAA GGGCTTCACT GGAGATGCAG CCTACTTTGG ATATGGAACC TAGACAGGAA
    CTGGAAAAAG AGACTTGTGT GGTGGTGAAG CCCAGTGCAG AGATGAAGCC TCCTGTGGAT
    GTGGAGGTGG ACACTGGTCT ACCCAAACTA GAAGAGCCAG ATGAAACAGG GCCCCAAATA
    GGTTTGGTGA TAGAACCTAC TGAGAGCCAG TTTGCCCAAC AACCTGAGGA GAGAAAGGAG
    GCTGAAAACA CTGAACCAGG AGTGGAGCCT CCAGATCGCA TCAGACCGGT ATACTCTGGG
    AAATTTTTTG ATCGGATGCC TTGCTGGCCA AGTGCAGGGA AAGTTATTCC TATTGGATAC
    AGAGTTGCAA CCTGTTTGAC TGAAAAACTT CCCAGGCTAA TTACTCCACC TGAGGCAAAA
    AAATTTTTCA ACTATAGAAA CCCACCAGCT GGAGCCGAAA GAGTATTTTA TGGCAGAGCA
    AATGATCCTC AGATTGCTCC TTATTTGACA CATGGAATAA GATCTAGAAT TTCAATACCG
    GCAAAGGTAC TGATAAACCC ACAGCCTGTA ACGACATTTC AACAGAAAAT TAAAGATAAA
    AAGGAATCAA TATATTTTAG TAATCAACGA GCACCACTAG GGAAATCTCA TGATCAAACG
    CCAGGATTAC CTAAAGGTCT GGATGTCCTC AATATGACAT TTGGGACAGC AGTCATCAGA
    GAATTATCTG CTAGAGATGT AGTGAATCCA CCAAAACCCT ATGAAGAAGT AGTTAAAGAA
    GGAAAAGAAG GACATGATCT GTATATTGTT TCTCACAATG ATTATTATGC AGGGGACAAT
    AATATTGTGA GGATTAGGAA AAGAGGAGCT AAGTTTGTAT CCAAGAGAGT TGATGATTTC
    AAAGAAAAGT TTCAACATGA ACTTGGAAGA GTTTTAGATC CCATTGCAGA AACAATGAAT
    ATTCCCCCAG ACCACACATT TGGAGCTTGT CTCCATGCTG AAGAGTATGG AGCTGGTGAT
    CTCATCCATA ACAGACTTCC AAGTGAATAT CTTCAAGGCA AGGATAGACA GAGAGCCCTG
    ATTGCAGCAG TGCGACATCA CTTGAAGAAA GTTAACTACC AAAACTTTGA CACTTTGCTG
    GCAGCCTTCA GGCATTATGA TAAGAATGGA GATGGGGTGA TAGATAAAGC CGAGCTTCGG
    GAGGCTTGTG AACAGGTCAG CTTGCACTTA GATGAGAAGC TCCTGGACCA GCTATTTGAC
    TACTGTGATG TGGATAATGA TGGGCTGATT AACTACCTAG AATTTGCAAA TTTTCTTAAC
    TGGAAGGATA AAATGCCTCT TAAAGAGTAT GAAGAGAGGG TCATAATTAA AGGTAGAAAA
    CCAGATTGTA CAAACCGTAC TGAGGCTAAT GTGGAAGAAT CTGAACCAAC TCTCCTAATA
    AAACCAGAAG ATATAGTCTT AAAAGAACCA GGGAGCTCAG AAAAGACTCT CCGGACGCTT
    CTGAGGCCAA GTGATAAAGT TTCTAATTAC TATAAGACAA CTTCTTCTGA GATCAGTGCG
    GTTGTAGGAG CTATTCCTTC TACTTGTTAT CCCATCTATG GTGTTCCAAC CATTCGGTCT
    GATATTCCTG CCCCCCGAAT TCGTCGCATC AGTGATAGAA CTAATTATGG TGAAGAGGGC
    AATGCCTATT CACTACTACA TCCTACTGTC TTTGGCCAGA AAGGAGTGTT TGAAAGAGAC
    TTCTTCAAGA CCAGATCAAA AAAAGAGATT GCAGAGATAT TATGTAACAT TGGTGTTAAG
    CTTTCTGATG AAGAATTTGA AAATGTATGG AATCTTGCAT CAAAAAAACA CCACAGAGGA
    GAAGTTTGTG TTGAAACCAT CAGAAATGTT CTGGATGAGC TACAACATGC AGACAGAATC
    AAGTGTAAAA CAAGCATGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe42486
    Clone ID Related Accession (Same CDS sequence) XM_014106569.1 , XM_014106569.2
    Accession Version XM_014106569.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2541bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product EF-hand domain-containing family member B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006605.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014106569.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGTGTTCTT TTGTGAGACT GGGGTCCCCT AAAGGGTTGA AGCACCACAA GATATTAGGA 
    ACTTCCCTTG AGCCAGTTAC AGCTTTTCCT TTAGAGATGA GTGACGTTAA AGGAATGAGG
    GTGTCAGAGA GGAAATCTTC TCGGAGCATG GAGGTTGGGC TCACCCACGA AGGAAAGGAT
    GACTTAGGAG AGACGGGGGT CCTTATGGGA GCAAGATCTC CCATAAGGGA GTTAGGGTTC
    AGAGTGGGAT TAGGAAAAGA AGATTCCTCA TGCAGGGAGA GCCTCCTGGT TGGTAACAAA
    TTTGAAGAAA GGATAACATC ACCAGAAACA AAATTTCCAT TGGGGATGGA GCTTGAAATG
    AAATTAAAAA GACAGAATCT TTCTGGAACC CTCCCGGAGA GGAGTAATTT AGGAGTCTAT
    GGGGTCTCAG CCTCCCAGGA AACAAAATCT TCTCCAGGTA TGTTGCCAGG AAGAAAGGGT
    GTAGAGAATG AGTATCTTAT GGCAGGATGT TCTGGAAGGA TAATACAGCC TCACTTGGGC
    ATGGTATGTG AAAATCCACA GACTCTAAGG AGCAGGAAAG GTTCTGTGGC TGGTTCCTCT
    GAAGGGTTAA GGGCTTCACT GGAGATGCAG CCTACTTTGG ATATGGAACC TAGACAGGAA
    CTGGAAAAAG AGACTTGTGT GGTGGTGAAG CCCAGTGCAG AGATGAAGCC TCCTGTGGAT
    GTGGAGGTGG ACACTGGTCT ACCCAAACTA GAAGAGCCAG ATGAAACAGG GCCCCAAATA
    GGTTTGGTGA TAGAACCTAC TGAGAGCCAG TTTGCCCAAC AACCTGAGGA GAGAAAGGAG
    GCTGAAAACA CTGAACCAGG AGTGGAGCCT CCAGATCGCA TCAGACCGGT ATACTCTGGG
    AAATTTTTTG ATCGGATGCC TTGCTGGCCA AGTGCAGGGA AAGTTATTCC TATTGGATAC
    AGAGTTGCAA CCTGTTTGAC TGAAAAACTT CCCAGGCTAA TTACTCCACC TGAGGCAAAA
    AAATTTTTCA ACTATAGAAA CCCACCAGCT GGAGCCGAAA GAGTATTTTA TGGCAGAGCA
    AATGATCCTC AGATTGCTCC TTATTTGACA CATGGAATAA GATCTAGAAT TTCAATACCG
    GCAAAGGTAC TGATAAACCC ACAGCCTGTA ACGACATTTC AACAGAAAAT TAAAGATAAA
    AAGGAATCAA TATATTTTAG TAATCAACGA GCACCACTAG GGAAATCTCA TGATCAAACG
    CCAGGATTAC CTAAAGGTCT GGATGTCCTC AATATGACAT TTGGGACAGC AGTCATCAGA
    GAATTATCTG CTAGAGATGT AGTGAATCCA CCAAAACCCT ATGAAGAAGT AGTTAAAGAA
    GGAAAAGAAG GACATGATCT GTATATTGTT TCTCACAATG ATTATTATGC AGGGGACAAT
    AATATTGTGA GGATTAGGAA AAGAGGAGCT AAGTTTGTAT CCAAGAGAGT TGATGATTTC
    AAAGAAAAGT TTCAACATGA ACTTGGAAGA GTTTTAGATC CCATTGCAGA AACAATGAAT
    ATTCCCCCAG ACCACACATT TGGAGCTTGT CTCCATGCTG AAGAGTATGG AGCTGGTGAT
    CTCATCCATA ACAGACTTCC AAGTGAATAT CTTCAAGGCA AGGATAGACA GAGAGCCCTG
    ATTGCAGCAG TGCGACATCA CTTGAAGAAA GTTAACTACC AAAACTTTGA CACTTTGCTG
    GCAGCCTTCA GGCATTATGA TAAGAATGGA GATGGGGTGA TAGATAAAGC CGAGCTTCGG
    GAGGCTTGTG AACAGGTCAG CTTGCACTTA GATGAGAAGC TCCTGGACCA GCTATTTGAC
    TACTGTGATG TGGATAATGA TGGGCTGATT AACTACCTAG AATTTGCAAA TTTTCTTAAC
    TGGAAGGATA AAATGCCTCT TAAAGAGTAT GAAGAGAGGG TCATAATTAA AGGTAGAAAA
    CCAGATTGTA CAAACCGTAC TGAGGCTAAT GTGGAAGAAT CTGAACCAAC TCTCCTAATA
    AAACCAGAAG ATATAGTCTT AAAAGAACCA GGGAGCTCAG AAAAGACTCT CCGGACGCTT
    CTGAGGCCAA GTGATAAAGT TTCTAATTAC TATAAGACAA CTTCTTCTGA GATCAGTGCG
    GTTGTAGGAG CTATTCCTTC TACTTGTTAT CCCATCTATG GTGTTCCAAC CATTCGGTCT
    GATATTCCTG CCCCCCGAAT TCGTCGCATC AGTGATAGAA CTAATTATGG TGAAGAGGGC
    AATGCCTATT CACTACTACA TCCTACTGTC TTTGGCCAGA AAGGAGTGTT TGAAAGAGAC
    TTCTTCAAGA CCAGATCAAA AAAAGAGATT GCAGAGATAT TATGTAACAT TGGTGTTAAG
    CTTTCTGATG AAGAATTTGA AAATGTATGG AATCTTGCAT CAAAAAAACA CCACAGAGGA
    GAAGTTTGTG TTGAAACCAT CAGAAATGTT CTGGATGAGC TACAACATGC AGACAGAATC
    AAGTGTAAAA CAAGCATGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013962044.1
    CDS220..2760
    Translation

    Target ORF information:

    RefSeq Version XM_014106569.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris EF-hand domain family member B (EFHB), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014106569.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGTGTTCTT TTGTGAGACT GGGGTCCCCT AAAGGGTTGA AGCACCACAA GATATTAGGA 
    ACTTCCCTTG AGCCAGTTAC AGCTTTTCCT TTAGAGATGA GTGACGTTAA AGGAATGAGG
    GTGTCAGAGA GGAAATCTTC TCGGAGCATG GAGGTTGGGC TCACCCACGA AGGAAAGGAT
    GACTTAGGAG AGACGGGGGT CCTTATGGGA GCAAGATCTC CCATAAGGGA GTTAGGGTTC
    AGAGTGGGAT TAGGAAAAGA AGATTCCTCA TGCAGGGAGA GCCTCCTGGT TGGTAACAAA
    TTTGAAGAAA GGATAACATC ACCAGAAACA AAATTTCCAT TGGGGATGGA GCTTGAAATG
    AAATTAAAAA GACAGAATCT TTCTGGAACC CTCCCGGAGA GGAGTAATTT AGGAGTCTAT
    GGGGTCTCAG CCTCCCAGGA AACAAAATCT TCTCCAGGTA TGTTGCCAGG AAGAAAGGGT
    GTAGAGAATG AGTATCTTAT GGCAGGATGT TCTGGAAGGA TAATACAGCC TCACTTGGGC
    ATGGTATGTG AAAATCCACA GACTCTAAGG AGCAGGAAAG GTTCTGTGGC TGGTTCCTCT
    GAAGGGTTAA GGGCTTCACT GGAGATGCAG CCTACTTTGG ATATGGAACC TAGACAGGAA
    CTGGAAAAAG AGACTTGTGT GGTGGTGAAG CCCAGTGCAG AGATGAAGCC TCCTGTGGAT
    GTGGAGGTGG ACACTGGTCT ACCCAAACTA GAAGAGCCAG ATGAAACAGG GCCCCAAATA
    GGTTTGGTGA TAGAACCTAC TGAGAGCCAG TTTGCCCAAC AACCTGAGGA GAGAAAGGAG
    GCTGAAAACA CTGAACCAGG AGTGGAGCCT CCAGATCGCA TCAGACCGGT ATACTCTGGG
    AAATTTTTTG ATCGGATGCC TTGCTGGCCA AGTGCAGGGA AAGTTATTCC TATTGGATAC
    AGAGTTGCAA CCTGTTTGAC TGAAAAACTT CCCAGGCTAA TTACTCCACC TGAGGCAAAA
    AAATTTTTCA ACTATAGAAA CCCACCAGCT GGAGCCGAAA GAGTATTTTA TGGCAGAGCA
    AATGATCCTC AGATTGCTCC TTATTTGACA CATGGAATAA GATCTAGAAT TTCAATACCG
    GCAAAGGTAC TGATAAACCC ACAGCCTGTA ACGACATTTC AACAGAAAAT TAAAGATAAA
    AAGGAATCAA TATATTTTAG TAATCAACGA GCACCACTAG GGAAATCTCA TGATCAAACG
    CCAGGATTAC CTAAAGGTCT GGATGTCCTC AATATGACAT TTGGGACAGC AGTCATCAGA
    GAATTATCTG CTAGAGATGT AGTGAATCCA CCAAAACCCT ATGAAGAAGT AGTTAAAGAA
    GGAAAAGAAG GACATGATCT GTATATTGTT TCTCACAATG ATTATTATGC AGGGGACAAT
    AATATTGTGA GGATTAGGAA AAGAGGAGCT AAGTTTGTAT CCAAGAGAGT TGATGATTTC
    AAAGAAAAGT TTCAACATGA ACTTGGAAGA GTTTTAGATC CCATTGCAGA AACAATGAAT
    ATTCCCCCAG ACCACACATT TGGAGCTTGT CTCCATGCTG AAGAGTATGG AGCTGGTGAT
    CTCATCCATA ACAGACTTCC AAGTGAATAT CTTCAAGGCA AGGATAGACA GAGAGCCCTG
    ATTGCAGCAG TGCGACATCA CTTGAAGAAA GTTAACTACC AAAACTTTGA CACTTTGCTG
    GCAGCCTTCA GGCATTATGA TAAGAATGGA GATGGGGTGA TAGATAAAGC CGAGCTTCGG
    GAGGCTTGTG AACAGGTCAG CTTGCACTTA GATGAGAAGC TCCTGGACCA GCTATTTGAC
    TACTGTGATG TGGATAATGA TGGGCTGATT AACTACCTAG AATTTGCAAA TTTTCTTAAC
    TGGAAGGATA AAATGCCTCT TAAAGAGTAT GAAGAGAGGG TCATAATTAA AGGTAGAAAA
    CCAGATTGTA CAAACCGTAC TGAGGCTAAT GTGGAAGAAT CTGAACCAAC TCTCCTAATA
    AAACCAGAAG ATATAGTCTT AAAAGAACCA GGGAGCTCAG AAAAGACTCT CCGGACGCTT
    CTGAGGCCAA GTGATAAAGT TTCTAATTAC TATAAGACAA CTTCTTCTGA GATCAGTGCG
    GTTGTAGGAG CTATTCCTTC TACTTGTTAT CCCATCTATG GTGTTCCAAC CATTCGGTCT
    GATATTCCTG CCCCCCGAAT TCGTCGCATC AGTGATAGAA CTAATTATGG TGAAGAGGGC
    AATGCCTATT CACTACTACA TCCTACTGTC TTTGGCCAGA AAGGAGTGTT TGAAAGAGAC
    TTCTTCAAGA CCAGATCAAA AAAAGAGATT GCAGAGATAT TATGTAACAT TGGTGTTAAG
    CTTTCTGATG AAGAATTTGA AAATGTATGG AATCTTGCAT CAAAAAAACA CCACAGAGGA
    GAAGTTTGTG TTGAAACCAT CAGAAATGTT CTGGATGAGC TACAACATGC AGACAGAATC
    AAGTGTAAAA CAAGCATGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.