NALCN cDNA ORF clone, Canis lupus familiaris(dog)

The following NALCN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NALCN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe296220 XM_022408131.1
Latest version!
Canis lupus familiaris sodium leak channel, non-selective (NALCN), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe296219 XM_022408130.1
Latest version!
Canis lupus familiaris sodium leak channel, non-selective (NALCN), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe296221 XM_022408132.1
Latest version!
Canis lupus familiaris sodium leak channel, non-selective (NALCN), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe23713 XM_542654.6
Latest version!
Canis lupus familiaris sodium leak channel, non-selective (NALCN), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe23714 XM_005634060.2
Latest version!
Canis lupus familiaris sodium leak channel, non selective (NALCN), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
View Old Accession Versions >>
OCe23713 XM_542654.5 Canis lupus familiaris sodium leak channel, non selective (NALCN), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe296220
    Clone ID Related Accession (Same CDS sequence) XM_022408131.1
    Accession Version XM_022408131.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4362bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product sodium leak channel non-selective protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006604.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTACAGAG CTATTGACAG CTTTCCCCGC TGGCGCTCCT ACTTCTACTT CATCACTCTT 
    ATCTTCTTCC TGGCCTGGCT CGTTAAGAAT GTATTTATCG CTGTCATCAT TGAAACATTT
    GCAGAAATCA GGGTGCAGTT TCAACAAATG TGGGGATCGA GAAGCAATAC TACGTCAACG
    GCCACGACGC AGATGTTTCA TGAAGACGCT GCCGGCGGAT GGCAGCTGGT AGCTGTGGAT
    GTCAACAAGC CCCAGGGACG CGCCCCAGCC TGCCTCCAGC AAATGATGCG GTCATCAGTT
    TTCCACATGT TCATTCTGAG CATGGTGACT GTGGATGTGA TAGTGGCTGC AAGCAACTAC
    CACAAAGGAG AAAGCTTCAG AAGGCAGTAC GATGAGTTCT ATCTTGCAGA GGTGGCTTTC
    ACAGTACTTT TTGATTTGGA AGCACTTCTG AAGATATGGT GTTTAGGATT TACTGGATAT
    ATCAGCTCTT CTCTCCACAA ATTTGAACTA CTACTCGTAA TTGGAACTAC TCTTCATGTA
    TATCCAGATC TTTATCATTC TCAGTTCACA TACTTTCAGG TTCTTCGGGT AGTTCGACTT
    ATTAAGATCT CACCTGCACT AGAAGACTTT GTGTACAAGA TATTTGGTCC TGGAAAAAAG
    CTTGGGAGTT TGGTTGTATT TACTGCCAGC CTCTTGATTG TTATGTCAGC AATTAGTCTG
    CAGATGTTCT GCTTTGTCGA AGAACTGGAC AGGTTTACAA CGTTTCCAAG GGCATTTATG
    TCCATGTTCC AGATCCTCAC CCAGGAAGGA TGGGTAGACG TGATGGACCA AACCTTGAAT
    GCTGTGGGAC ACATGTGGGC ACCTGTGGTG GCCATCTATT TCATTTTGTA CCATCTCTTT
    GCCACTCTGA TCCTCCTGAG TTTGTTTGTT GCGGTTATTT TGGACAACTT AGAACTTGAT
    GAAGATCTAA AGAAGCTTAA ACAATTAAAA CAAAGTGAAG CGAATGCGGA CACCAAAGAA
    AAGCTCCCCT TGCGACTTCG AATCTTTGAA AAATTTCCAA ACAGACCGCA AATGGTGAAA
    ATCTCAAAGC TTCCTTCAGA TTTTACAGTT CCTAAAATCA GGGAAAGTTT CATGAAGCAG
    TTTATTGATC GCCAGCAACA GGACACCTGC TGCCTCTTAC GAAGCCTTCC GTCCACCTCT
    TCTTCATCGT GTGATCACTC CAAACGGTCT GCAATTGAAG ACAACAAATA CATTGACCAA
    AAACTTCGCA AGTCTGTTTT CAGCATCAGG GCAAGGAATC TTCTAGAGAA GGAGACTGCA
    GTCACTAAAA TCTTAAGAGC TTGCACTCGA CAGCGCATGC TGAGCGGATC ATTTGAGGGG
    CAGCCAGCAA AGGAAAGGTC AATTCTCAGT GTGCAGCATC ATATCCGCCA GGAGCGCAGG
    TCACTGAGAC ATGGATCCAA CAGCCAGAGG ATCAGCAGGG GCAAATCTCT TGAAACCCTG
    ACTCAAGACC ACTCCAATAC AGTGAGATAC AGAAATGCAC AGAGAGAAGA CAGTGAAATC
    AAGATGATCC AGGAAAAAAA GGAGCAAGCA GAAATGAAAA GGAAAGTGCA GGAAGAGGAG
    CTGAGAGAGA ATCACCCATA CTTCGACAAG CCACTGTTCA TAGTTGGACG GGAGCACAGG
    TTCAGAAACT TCTGCCGGGT GGTGGTCCGG GCACGCTTCA ATGCATCTAA AACAGATCCC
    GTCACAGGAG CTGTGAAAAA TACAAAGTAC CATCAACTTT ATGATTTGCT GGGCTTGGTG
    ACTTACCTGG ACTGGGTCAT GATCATCGTA ACCATCTGTT CCTGCATTTC CATGATGTTT
    GAGTCCCCCT TTCGAAGAGT CATGCACGCC CCAACTTTGC AGATTGCCGA GTACGTGTTT
    GTGATATTCA TGAGCATCGA GCTTAATCTG AAGATTATGG CAGATGGCTT ATTTTTCACT
    CCAACTGCTG TCATCAGAGA CTTTGGTGGA GTCATGGACA TATTTATATA TCTTGTAAGC
    TTGATATTTC TTTGTTGGAT GCCTCAAAAT GTGCCTGCTG AATCTGGAGC TCAGCTTTTA
    ATGGTTCTGC GGTGCCTGAG ACCTCTGCGA ATATTCAAAC TGGTGCCCCA GATGAGGAAA
    GTTGTTCGAG AACTTTTCAG TGGCTTCAAG GAAATTTTCT TGGTCTCCAT TCTTTTGTTG
    ACATTAATGC TTGTATTTGC AAGCTTTGGA GTTCAGCTTT TTGCGGGGAA ACTGGCAAAG
    TGCAACGATC CCAACATTAT TAGAAGGGAA GATTGTAACG GCATATTCAG AATTAATGTA
    AGTGTGTCCA AGAACTTAAA TTTAAAATTA AGACCTGGAG AGAAAAAGCC TGGATTTTGG
    GTGCCCCGTG TTTGGGCAAA TCCTAGGAAT TTTAATTTTG ACAATGTGGG AAATGCTATG
    CTGGCATTAT TTGAAGTGCT GTCCCTGAAA GGCTGGGTGG AAGTGAGGGA TGTTATCATT
    CATCGTGTGG GGCCGATGCA TGGGATCTAT ATTCATGTTT TTGTATTCCT GGGTTGCATG
    ATTGGACTGA CCCTTTTTGT TGGAGTAGTT ATTGCTAATT TCAATGAAAA CAAGGGGACA
    GCTCTGCTGA CTGTAGATCA GAGAAGATGG GAAGATCTGA AGAGCCGATT GAAGATAGCC
    CAGCCTCTTC ATCTCCCACC TCGCCCGGAT AATGATGGTT TTAGAGCTAA GATGTATGAC
    ATCACCCAGC ATCCGTTCTT TAAGAGGACA ATCGCATTGC TTGTTTTGGC CCAGTCGGTG
    CTGCTGTCCG TCAAGTGGGA CGTGGAGGAC CCAGTGACTG TCCCTTTGGC AACCATGTCA
    GTTGTTTTCA CCTTCATCTT TGTTCTAGAG GTTACAATGA AGATCATAGC CATGTCGCCC
    GCCGGCTTCT GGCAAAGCAG AAGAAACCGA TACGACCTGC TGGTGACGTC ACTTGGTGTG
    GTCTGGGTGG TACTTCACTT TGCCCTCCTG AACGCATATA CCTACATGAT GGGTGCCTGC
    GTGATTGTCT TCCGGTTTTT CTCTATCTGT GGAAAGCATG TAACGCTAAA GATGCTCCTC
    CTGACAGTGG TTGTCAGCAT GTATAAAAGC TTCTTCATCA TAGTAGGGAT GTTCCTTTTG
    CTCTTGTGTT ATGCTTTTGC TGGAGTTGTT TTATTTGGTA CTGTGAAATA TGGAGAGAAC
    ATTAACAGGC ATGCCAACTT TTCTTCAGCT GGCAAAGCCA TCACCGTACT GTTCCGAATT
    GTCACAGGCG AAGACTGGAA TAAGATTATG CATGACTGTA TGGTCCAGCC TCCCTTTTGC
    ACACCAGATG AATTCACATA CTGGGCAACA GACTGTGGAA ATTATGCCGG GGCGCTTATG
    TATTTCTGTT CATTTTATGT CATCATTGCC TACATCATGC TAAATCTGCT TGTAGCCATC
    ATTGTGGAGA ATTTCTCCTT GTTTTATTCC ACGGAGGAGG ACCAGCTCCT AAGTTACAAT
    GATCTCCGCC ACTTTCAAAT CATATGGAAT ATGGTGGATG ATAAACGAGA GGGGGTGATT
    CCAACATTCC GGGTAAAGTT CCTGCTGAGG CTGCTGCGGG GGAGGCTGGA GGTGGATCTA
    GACAAAGACA AGCTCCTGTT TAAGCACATG TGCTATGAAA TGGAGAGGCT GCATAATGGC
    GGCGATGTCA CTTTCCATGA CGTGCTCAGC ATGCTCTCTT ACCGGTCAGT TGACATCCGG
    AAGAGTTTGC AGTTGGAGGA GCTGCTGGCC AGGGAGCAGC TGGAGTACAC CATCGAGGAG
    GAGGTGGCCA AGCAGACCAT CCGCATGTGG CTGAAGAAGT GCTTAAAGCG CATTAGAGCT
    AAACAGCAGC AGTCCTGCAG CATCATCCAC AGCCTGAGGG AGAGCCAACA GCAGGAGCTG
    AGCCGCTTCC TGAATCCTCC CAGTATCGAG ACCACCCAGC CCAGTGAGGA CATGAACGCT
    AACAGTCAGG ATAATAATAT GCAACCTGAG ACCAGCAGCC AGCAGCAGCT TCTCAGCCCC
    ACGCTGTCGG ACAGGGGGGG CGGCCGGCAG GACTCCGCGG ACCCCGGGAA GCCCCAGAGG
    AAGATCGGAC AGTGGCGGCT GCCCTCAGCA CCAAAACCAA TAAGCCACTC AGTGTCTTCG
    GTCAACTTAC GATTTGCAGG GCGCACAACA ATGAAATCCG TAGTGTGCAA AATGAACCCC
    ATGACCGACA CGGCGTCGTG TGGTTCAGAG GTTAAGAAGT GGTGGACGCG GCAGCTGACA
    GTGGAAAGCG ACGAAAGTGG CGATGACCTC CTGGATATTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022263839.1
    CDS79..4440
    Translation

    Target ORF information:

    RefSeq Version XM_022408131.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris sodium leak channel, non-selective (NALCN), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022408131.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTACAGAG CTATTGACAG CTTTCCCCGC TGGCGCTCCT ACTTCTACTT CATCACTCTT 
    ATCTTCTTCC TGGCCTGGCT CGTTAAGAAT GTATTTATCG CTGTCATCAT TGAAACATTT
    GCAGAAATCA GGGTGCAGTT TCAACAAATG TGGGGATCGA GAAGCAATAC TACGTCAACG
    GCCACGACGC AGATGTTTCA TGAAGACGCT GCCGGCGGAT GGCAGCTGGT AGCTGTGGAT
    GTCAACAAGC CCCAGGGACG CGCCCCAGCC TGCCTCCAGC AAATGATGCG GTCATCAGTT
    TTCCACATGT TCATTCTGAG CATGGTGACT GTGGATGTGA TAGTGGCTGC AAGCAACTAC
    CACAAAGGAG AAAGCTTCAG AAGGCAGTAC GATGAGTTCT ATCTTGCAGA GGTGGCTTTC
    ACAGTACTTT TTGATTTGGA AGCACTTCTG AAGATATGGT GTTTAGGATT TACTGGATAT
    ATCAGCTCTT CTCTCCACAA ATTTGAACTA CTACTCGTAA TTGGAACTAC TCTTCATGTA
    TATCCAGATC TTTATCATTC TCAGTTCACA TACTTTCAGG TTCTTCGGGT AGTTCGACTT
    ATTAAGATCT CACCTGCACT AGAAGACTTT GTGTACAAGA TATTTGGTCC TGGAAAAAAG
    CTTGGGAGTT TGGTTGTATT TACTGCCAGC CTCTTGATTG TTATGTCAGC AATTAGTCTG
    CAGATGTTCT GCTTTGTCGA AGAACTGGAC AGGTTTACAA CGTTTCCAAG GGCATTTATG
    TCCATGTTCC AGATCCTCAC CCAGGAAGGA TGGGTAGACG TGATGGACCA AACCTTGAAT
    GCTGTGGGAC ACATGTGGGC ACCTGTGGTG GCCATCTATT TCATTTTGTA CCATCTCTTT
    GCCACTCTGA TCCTCCTGAG TTTGTTTGTT GCGGTTATTT TGGACAACTT AGAACTTGAT
    GAAGATCTAA AGAAGCTTAA ACAATTAAAA CAAAGTGAAG CGAATGCGGA CACCAAAGAA
    AAGCTCCCCT TGCGACTTCG AATCTTTGAA AAATTTCCAA ACAGACCGCA AATGGTGAAA
    ATCTCAAAGC TTCCTTCAGA TTTTACAGTT CCTAAAATCA GGGAAAGTTT CATGAAGCAG
    TTTATTGATC GCCAGCAACA GGACACCTGC TGCCTCTTAC GAAGCCTTCC GTCCACCTCT
    TCTTCATCGT GTGATCACTC CAAACGGTCT GCAATTGAAG ACAACAAATA CATTGACCAA
    AAACTTCGCA AGTCTGTTTT CAGCATCAGG GCAAGGAATC TTCTAGAGAA GGAGACTGCA
    GTCACTAAAA TCTTAAGAGC TTGCACTCGA CAGCGCATGC TGAGCGGATC ATTTGAGGGG
    CAGCCAGCAA AGGAAAGGTC AATTCTCAGT GTGCAGCATC ATATCCGCCA GGAGCGCAGG
    TCACTGAGAC ATGGATCCAA CAGCCAGAGG ATCAGCAGGG GCAAATCTCT TGAAACCCTG
    ACTCAAGACC ACTCCAATAC AGTGAGATAC AGAAATGCAC AGAGAGAAGA CAGTGAAATC
    AAGATGATCC AGGAAAAAAA GGAGCAAGCA GAAATGAAAA GGAAAGTGCA GGAAGAGGAG
    CTGAGAGAGA ATCACCCATA CTTCGACAAG CCACTGTTCA TAGTTGGACG GGAGCACAGG
    TTCAGAAACT TCTGCCGGGT GGTGGTCCGG GCACGCTTCA ATGCATCTAA AACAGATCCC
    GTCACAGGAG CTGTGAAAAA TACAAAGTAC CATCAACTTT ATGATTTGCT GGGCTTGGTG
    ACTTACCTGG ACTGGGTCAT GATCATCGTA ACCATCTGTT CCTGCATTTC CATGATGTTT
    GAGTCCCCCT TTCGAAGAGT CATGCACGCC CCAACTTTGC AGATTGCCGA GTACGTGTTT
    GTGATATTCA TGAGCATCGA GCTTAATCTG AAGATTATGG CAGATGGCTT ATTTTTCACT
    CCAACTGCTG TCATCAGAGA CTTTGGTGGA GTCATGGACA TATTTATATA TCTTGTAAGC
    TTGATATTTC TTTGTTGGAT GCCTCAAAAT GTGCCTGCTG AATCTGGAGC TCAGCTTTTA
    ATGGTTCTGC GGTGCCTGAG ACCTCTGCGA ATATTCAAAC TGGTGCCCCA GATGAGGAAA
    GTTGTTCGAG AACTTTTCAG TGGCTTCAAG GAAATTTTCT TGGTCTCCAT TCTTTTGTTG
    ACATTAATGC TTGTATTTGC AAGCTTTGGA GTTCAGCTTT TTGCGGGGAA ACTGGCAAAG
    TGCAACGATC CCAACATTAT TAGAAGGGAA GATTGTAACG GCATATTCAG AATTAATGTA
    AGTGTGTCCA AGAACTTAAA TTTAAAATTA AGACCTGGAG AGAAAAAGCC TGGATTTTGG
    GTGCCCCGTG TTTGGGCAAA TCCTAGGAAT TTTAATTTTG ACAATGTGGG AAATGCTATG
    CTGGCATTAT TTGAAGTGCT GTCCCTGAAA GGCTGGGTGG AAGTGAGGGA TGTTATCATT
    CATCGTGTGG GGCCGATGCA TGGGATCTAT ATTCATGTTT TTGTATTCCT GGGTTGCATG
    ATTGGACTGA CCCTTTTTGT TGGAGTAGTT ATTGCTAATT TCAATGAAAA CAAGGGGACA
    GCTCTGCTGA CTGTAGATCA GAGAAGATGG GAAGATCTGA AGAGCCGATT GAAGATAGCC
    CAGCCTCTTC ATCTCCCACC TCGCCCGGAT AATGATGGTT TTAGAGCTAA GATGTATGAC
    ATCACCCAGC ATCCGTTCTT TAAGAGGACA ATCGCATTGC TTGTTTTGGC CCAGTCGGTG
    CTGCTGTCCG TCAAGTGGGA CGTGGAGGAC CCAGTGACTG TCCCTTTGGC AACCATGTCA
    GTTGTTTTCA CCTTCATCTT TGTTCTAGAG GTTACAATGA AGATCATAGC CATGTCGCCC
    GCCGGCTTCT GGCAAAGCAG AAGAAACCGA TACGACCTGC TGGTGACGTC ACTTGGTGTG
    GTCTGGGTGG TACTTCACTT TGCCCTCCTG AACGCATATA CCTACATGAT GGGTGCCTGC
    GTGATTGTCT TCCGGTTTTT CTCTATCTGT GGAAAGCATG TAACGCTAAA GATGCTCCTC
    CTGACAGTGG TTGTCAGCAT GTATAAAAGC TTCTTCATCA TAGTAGGGAT GTTCCTTTTG
    CTCTTGTGTT ATGCTTTTGC TGGAGTTGTT TTATTTGGTA CTGTGAAATA TGGAGAGAAC
    ATTAACAGGC ATGCCAACTT TTCTTCAGCT GGCAAAGCCA TCACCGTACT GTTCCGAATT
    GTCACAGGCG AAGACTGGAA TAAGATTATG CATGACTGTA TGGTCCAGCC TCCCTTTTGC
    ACACCAGATG AATTCACATA CTGGGCAACA GACTGTGGAA ATTATGCCGG GGCGCTTATG
    TATTTCTGTT CATTTTATGT CATCATTGCC TACATCATGC TAAATCTGCT TGTAGCCATC
    ATTGTGGAGA ATTTCTCCTT GTTTTATTCC ACGGAGGAGG ACCAGCTCCT AAGTTACAAT
    GATCTCCGCC ACTTTCAAAT CATATGGAAT ATGGTGGATG ATAAACGAGA GGGGGTGATT
    CCAACATTCC GGGTAAAGTT CCTGCTGAGG CTGCTGCGGG GGAGGCTGGA GGTGGATCTA
    GACAAAGACA AGCTCCTGTT TAAGCACATG TGCTATGAAA TGGAGAGGCT GCATAATGGC
    GGCGATGTCA CTTTCCATGA CGTGCTCAGC ATGCTCTCTT ACCGGTCAGT TGACATCCGG
    AAGAGTTTGC AGTTGGAGGA GCTGCTGGCC AGGGAGCAGC TGGAGTACAC CATCGAGGAG
    GAGGTGGCCA AGCAGACCAT CCGCATGTGG CTGAAGAAGT GCTTAAAGCG CATTAGAGCT
    AAACAGCAGC AGTCCTGCAG CATCATCCAC AGCCTGAGGG AGAGCCAACA GCAGGAGCTG
    AGCCGCTTCC TGAATCCTCC CAGTATCGAG ACCACCCAGC CCAGTGAGGA CATGAACGCT
    AACAGTCAGG ATAATAATAT GCAACCTGAG ACCAGCAGCC AGCAGCAGCT TCTCAGCCCC
    ACGCTGTCGG ACAGGGGGGG CGGCCGGCAG GACTCCGCGG ACCCCGGGAA GCCCCAGAGG
    AAGATCGGAC AGTGGCGGCT GCCCTCAGCA CCAAAACCAA TAAGCCACTC AGTGTCTTCG
    GTCAACTTAC GATTTGCAGG GCGCACAACA ATGAAATCCG TAGTGTGCAA AATGAACCCC
    ATGACCGACA CGGCGTCGTG TGGTTCAGAG GTTAAGAAGT GGTGGACGCG GCAGCTGACA
    GTGGAAAGCG ACGAAAGTGG CGATGACCTC CTGGATATTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe296219
    Clone ID Related Accession (Same CDS sequence) XM_022408130.1
    Accession Version XM_022408130.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5280bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product sodium leak channel non-selective protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006604.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAGGAGAC AAGACGACCC CCCAGAGACA CTCAATATCA TGCTTCCTGC TGGCACATCT 
    AGGAACCACA CTGCGACTGC AGTTTTGTAC TTGCTCACCA AAGCTAACTT CAGCATGCTC
    AAAAGGAAGC AGAGCTCCAG GGTGGAGGCC CAGCCAGTTA CTGACTTTGG TCCCGACGAA
    TCTCTGTCCG ACAACGCTGA CATACTCTGG ATTAATAAGC CATGGGTTCA CTCTTTGCTG
    CGCATCTGTG CCATCATCAG TGTCATTTCT GTCTGTATGA ACACACCCAT GACGTTCGAA
    CACTACCCCC CTCTTCAGTA TGTGACTTTT ACTCTGGATA CTTTACTGAT GTTTCTCTAC
    ACAGCAGAGA TGATAGCAAA AATGCACATC CGGGGTATTG TCAAGGGGGA TAGTTCCTAT
    GTGAAAGATC GCTGGTGTGT TTTTGATGGA TTTATGGTCT TCTGCCTTTG GGTTTCATTG
    GTGTTACAGG TATTTGAAAT TGCTGATATA GTTGACCAGA TGTCACCTTG GGGCATGTTG
    CGGATCCCAC GGCCACTAAT TATGATTCGG GCATTCCGGA TTTATTTCCG ATTTGAGCTA
    CCAAGGACCA GAATTACAAA CATTTTAAAG CGATCAGGAG AACAAATATG GAGCGTTTCA
    ATTTTCCTAC TTTTCTTTCT ACTTCTTTAT GGAATTTTAG GAGTTCAGAT GTTTGGAACA
    TTTACCTATC ACTGTGTAGT AAATGACACG AAACCAGGAA ACGTAACCTG GAATAGCTTA
    GCTATCCCAG ATACGCACTG CTCACCAGAG CTGGAGGAGG GCTACCAGTG CCCCCCAGGA
    TTTAAATGCA TGGACCTGGA AGACCTGGGA CTTAGCAGGC AAGAGCTGGG CTACAGTGGC
    TTTAATGAGA TAGGCACCAG CATCTTCACG GTCTATGAGG CCGCGTCCCA GGAAGGCTGG
    GTGTTCCTCA TGTACAGAGC TATTGACAGC TTTCCCCGCT GGCGCTCCTA CTTCTACTTC
    ATCACTCTTA TCTTCTTCCT GGCCTGGCTC GTTAAGAATG TATTTATCGC TGTCATCATT
    GAAACATTTG CAGAAATCAG GGTGCAGTTT CAACAAATGT GGGGATCGAG AAGCAATACT
    ACGTCAACGG CCACGACGCA GATGTTTCAT GAAGACGCTG CCGGCGGATG GCAGCTGGTA
    GCTGTGGATG TCAACAAGCC CCAGGGACGC GCCCCAGCCT GCCTCCAGCA AATGATGCGG
    TCATCAGTTT TCCACATGTT CATTCTGAGC ATGGTGACTG TGGATGTGAT AGTGGCTGCA
    AGCAACTACC ACAAAGGAGA AAGCTTCAGA AGGCAGTACG ATGAGTTCTA TCTTGCAGAG
    GTGGCTTTCA CAGTACTTTT TGATTTGGAA GCACTTCTGA AGATATGGTG TTTAGGATTT
    ACTGGATATA TCAGCTCTTC TCTCCACAAA TTTGAACTAC TACTCGTAAT TGGAACTACT
    CTTCATGTAT ATCCAGATCT TTATCATTCT CAGTTCACAT ACTTTCAGGT TCTTCGGGTA
    GTTCGACTTA TTAAGATCTC ACCTGCACTA GAAGACTTTG TGTACAAGAT ATTTGGTCCT
    GGAAAAAAGC TTGGGAGTTT GGTTGTATTT ACTGCCAGCC TCTTGATTGT TATGTCAGCA
    ATTAGTCTGC AGATGTTCTG CTTTGTCGAA GAACTGGACA GGTTTACAAC GTTTCCAAGG
    GCATTTATGT CCATGTTCCA GATCCTCACC CAGGAAGGAT GGGTAGACGT GATGGACCAA
    ACCTTGAATG CTGTGGGACA CATGTGGGCA CCTGTGGTGG CCATCTATTT CATTTTGTAC
    CATCTCTTTG CCACTCTGAT CCTCCTGAGT TTGTTTGTTG CGGTTATTTT GGACAACTTA
    GAACTTGATG AAGATCTAAA GAAGCTTAAA CAATTAAAAC AAAGTGAAGC GAATGCGGAC
    ACCAAAGAAA AGCTCCCCTT GCGACTTCGA ATCTTTGAAA AATTTCCAAA CAGACCGCAA
    ATGGTGAAAA TCTCAAAGCT TCCTTCAGAT TTTACAGTTC CTAAAATCAG GGAAAGTTTC
    ATGAAGCAGT TTATTGATCG CCAGCAACAG GACACCTGCT GCCTCTTACG AAGCCTTCCG
    TCCACCTCTT CTTCATCGTG TGATCACTCC AAACGGTCTG CAATTGAAGA CAACAAATAC
    ATTGACCAAA AACTTCGCAA GTCTGTTTTC AGCATCAGGG CAAGGAATCT TCTAGAGAAG
    GAGACTGCAG TCACTAAAAT CTTAAGAGCT TGCACTCGAC AGCGCATGCT GAGCGGATCA
    TTTGAGGGGC AGCCAGCAAA GGAAAGGTCA ATTCTCAGTG TGCAGCATCA TATCCGCCAG
    GAGCGCAGGT CACTGAGACA TGGATCCAAC AGCCAGAGGA TCAGCAGGGG CAAATCTCTT
    GAAACCCTGA CTCAAGACCA CTCCAATACA GTGAGATACA GAAATGCACA GAGAGAAGAC
    AGTGAAATCA AGATGATCCA GGAAAAAAAG GAGCAAGCAG AAATGAAAAG GAAAGTGCAG
    GAAGAGGAGC TGAGAGAGAA TCACCCATAC TTCGACAAGC CACTGTTCAT AGTTGGACGG
    GAGCACAGGT TCAGAAACTT CTGCCGGGTG GTGGTCCGGG CACGCTTCAA TGCATCTAAA
    ACAGATCCCG TCACAGGAGC TGTGAAAAAT ACAAAGTACC ATCAACTTTA TGATTTGCTG
    GGCTTGGTGA CTTACCTGGA CTGGGTCATG ATCATCGTAA CCATCTGTTC CTGCATTTCC
    ATGATGTTTG AGTCCCCCTT TCGAAGAGTC ATGCACGCCC CAACTTTGCA GATTGCCGAG
    TACGTGTTTG TGATATTCAT GAGCATCGAG CTTAATCTGA AGATTATGGC AGATGGCTTA
    TTTTTCACTC CAACTGCTGT CATCAGAGAC TTTGGTGGAG TCATGGACAT ATTTATATAT
    CTTGTAAGCT TGATATTTCT TTGTTGGATG CCTCAAAATG TGCCTGCTGA ATCTGGAGCT
    CAGCTTTTAA TGGTTCTGCG GTGCCTGAGA CCTCTGCGAA TATTCAAACT GGTGCCCCAG
    ATGAGGAAAG TTGTTCGAGA ACTTTTCAGT GGCTTCAAGG AAATTTTCTT GGTCTCCATT
    CTTTTGTTGA CATTAATGCT TGTATTTGCA AGCTTTGGAG TTCAGCTTTT TGCGGGGAAA
    CTGGCAAAGT GCAACGATCC CAACATTATT AGAAGGGAAG ATTGTAACGG CATATTCAGA
    ATTAATGTAA GTGTGTCCAA GAACTTAAAT TTAAAATTAA GACCTGGAGA GAAAAAGCCT
    GGATTTTGGG TGCCCCGTGT TTGGGCAAAT CCTAGGAATT TTAATTTTGA CAATGTGGGA
    AATGCTATGC TGGCATTATT TGAAGTGCTG TCCCTGAAAG GCTGGGTGGA AGTGAGGGAT
    GTTATCATTC ATCGTGTGGG GCCGATGCAT GGGATCTATA TTCATGTTTT TGTATTCCTG
    GGTTGCATGA TTGGACTGAC CCTTTTTGTT GGAGTAGTTA TTGCTAATTT CAATGAAAAC
    AAGGGGACAG CTCTGCTGAC TGTAGATCAG AGAAGATGGG AAGATCTGAA GAGCCGATTG
    AAGATAGCCC AGCCTCTTCA TCTCCCACCT CGCCCGGATA ATGATGGTTT TAGAGCTAAG
    ATGTATGACA TCACCCAGCA TCCGTTCTTT AAGAGGACAA TCGCATTGCT TGTTTTGGCC
    CAGTCGGTGC TGCTGTCCGT CAAGTGGGAC GTGGAGGACC CAGTGACTGT CCCTTTGGCA
    ACCATGTCAG TTGTTTTCAC CTTCATCTTT GTTCTAGAGG TTACAATGAA GATCATAGCC
    ATGTCGCCCG CCGGCTTCTG GCAAAGCAGA AGAAACCGAT ACGACCTGCT GGTGACGTCA
    CTTGGTGTGG TCTGGGTGGT ACTTCACTTT GCCCTCCTGA ACGCATATAC CTACATGATG
    GGTGCCTGCG TGATTGTCTT CCGGTTTTTC TCTATCTGTG GAAAGCATGT AACGCTAAAG
    ATGCTCCTCC TGACAGTGGT TGTCAGCATG TATAAAAGCT TCTTCATCAT AGTAGGGATG
    TTCCTTTTGC TCTTGTGTTA TGCTTTTGCT GGAGTTGTTT TATTTGGTAC TGTGAAATAT
    GGAGAGAACA TTAACAGGCA TGCCAACTTT TCTTCAGCTG GCAAAGCCAT CACCGTACTG
    TTCCGAATTG TCACAGGCGA AGACTGGAAT AAGATTATGC ATGACTGTAT GGTCCAGCCT
    CCCTTTTGCA CACCAGATGA ATTCACATAC TGGGCAACAG ACTGTGGAAA TTATGCCGGG
    GCGCTTATGT ATTTCTGTTC ATTTTATGTC ATCATTGCCT ACATCATGCT AAATCTGCTT
    GTAGCCATCA TTGTGGAGAA TTTCTCCTTG TTTTATTCCA CGGAGGAGGA CCAGCTCCTA
    AGTTACAATG ATCTCCGCCA CTTTCAAATC ATATGGAATA TGGTGGATGA TAAACGAGAG
    GGGGTGATTC CAACATTCCG GGTAAAGTTC CTGCTGAGGC TGCTGCGGGG GAGGCTGGAG
    GTGGATCTAG ACAAAGACAA GCTCCTGTTT AAGCACATGT GCTATGAAAT GGAGAGGCTG
    CATAATGGCG GCGATGTCAC TTTCCATGAC GTGCTCAGCA TGCTCTCTTA CCGGTCAGTT
    GACATCCGGA AGAGTTTGCA GTTGGAGGAG CTGCTGGCCA GGGAGCAGCT GGAGTACACC
    ATCGAGGAGG AGGTGGCCAA GCAGACCATC CGCATGTGGC TGAAGAAGTG CTTAAAGCGC
    ATTAGAGCTA AACAGCAGCA GTCCTGCAGC ATCATCCACA GCCTGAGGGA GAGCCAACAG
    CAGGAGCTGA GCCGCTTCCT GAATCCTCCC AGTATCGAGA CCACCCAGCC CAGTGAGGAC
    ATGAACGCTA ACAGTCAGGA TAATAATATG CAACCTGAGA CCAGCAGCCA GCAGCAGCTT
    CTCAGCCCCA CGCTGTCGGA CAGGGGGGGC GGCCGGCAGG ACTCCGCGGA CCCCGGGAAG
    CCCCAGAGGA AGATCGGACA GTGGCGGCTG CCCTCAGGGC GCACAACAAT GAAATCCGTA
    GTGTGCAAAA TGAACCCCAT GACCGACACG GCGTCGTGTG GTTCAGAGGT TAAGAAGTGG
    TGGACGCGGC AGCTGACAGT GGAAAGCGAC GAAAGTGGCG ATGACCTCCT GGATATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022263838.1
    CDS357..5636
    Translation

    Target ORF information:

    RefSeq Version XM_022408130.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris sodium leak channel, non-selective (NALCN), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022408130.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAGGAGAC AAGACGACCC CCCAGAGACA CTCAATATCA TGCTTCCTGC TGGCACATCT 
    AGGAACCACA CTGCGACTGC AGTTTTGTAC TTGCTCACCA AAGCTAACTT CAGCATGCTC
    AAAAGGAAGC AGAGCTCCAG GGTGGAGGCC CAGCCAGTTA CTGACTTTGG TCCCGACGAA
    TCTCTGTCCG ACAACGCTGA CATACTCTGG ATTAATAAGC CATGGGTTCA CTCTTTGCTG
    CGCATCTGTG CCATCATCAG TGTCATTTCT GTCTGTATGA ACACACCCAT GACGTTCGAA
    CACTACCCCC CTCTTCAGTA TGTGACTTTT ACTCTGGATA CTTTACTGAT GTTTCTCTAC
    ACAGCAGAGA TGATAGCAAA AATGCACATC CGGGGTATTG TCAAGGGGGA TAGTTCCTAT
    GTGAAAGATC GCTGGTGTGT TTTTGATGGA TTTATGGTCT TCTGCCTTTG GGTTTCATTG
    GTGTTACAGG TATTTGAAAT TGCTGATATA GTTGACCAGA TGTCACCTTG GGGCATGTTG
    CGGATCCCAC GGCCACTAAT TATGATTCGG GCATTCCGGA TTTATTTCCG ATTTGAGCTA
    CCAAGGACCA GAATTACAAA CATTTTAAAG CGATCAGGAG AACAAATATG GAGCGTTTCA
    ATTTTCCTAC TTTTCTTTCT ACTTCTTTAT GGAATTTTAG GAGTTCAGAT GTTTGGAACA
    TTTACCTATC ACTGTGTAGT AAATGACACG AAACCAGGAA ACGTAACCTG GAATAGCTTA
    GCTATCCCAG ATACGCACTG CTCACCAGAG CTGGAGGAGG GCTACCAGTG CCCCCCAGGA
    TTTAAATGCA TGGACCTGGA AGACCTGGGA CTTAGCAGGC AAGAGCTGGG CTACAGTGGC
    TTTAATGAGA TAGGCACCAG CATCTTCACG GTCTATGAGG CCGCGTCCCA GGAAGGCTGG
    GTGTTCCTCA TGTACAGAGC TATTGACAGC TTTCCCCGCT GGCGCTCCTA CTTCTACTTC
    ATCACTCTTA TCTTCTTCCT GGCCTGGCTC GTTAAGAATG TATTTATCGC TGTCATCATT
    GAAACATTTG CAGAAATCAG GGTGCAGTTT CAACAAATGT GGGGATCGAG AAGCAATACT
    ACGTCAACGG CCACGACGCA GATGTTTCAT GAAGACGCTG CCGGCGGATG GCAGCTGGTA
    GCTGTGGATG TCAACAAGCC CCAGGGACGC GCCCCAGCCT GCCTCCAGCA AATGATGCGG
    TCATCAGTTT TCCACATGTT CATTCTGAGC ATGGTGACTG TGGATGTGAT AGTGGCTGCA
    AGCAACTACC ACAAAGGAGA AAGCTTCAGA AGGCAGTACG ATGAGTTCTA TCTTGCAGAG
    GTGGCTTTCA CAGTACTTTT TGATTTGGAA GCACTTCTGA AGATATGGTG TTTAGGATTT
    ACTGGATATA TCAGCTCTTC TCTCCACAAA TTTGAACTAC TACTCGTAAT TGGAACTACT
    CTTCATGTAT ATCCAGATCT TTATCATTCT CAGTTCACAT ACTTTCAGGT TCTTCGGGTA
    GTTCGACTTA TTAAGATCTC ACCTGCACTA GAAGACTTTG TGTACAAGAT ATTTGGTCCT
    GGAAAAAAGC TTGGGAGTTT GGTTGTATTT ACTGCCAGCC TCTTGATTGT TATGTCAGCA
    ATTAGTCTGC AGATGTTCTG CTTTGTCGAA GAACTGGACA GGTTTACAAC GTTTCCAAGG
    GCATTTATGT CCATGTTCCA GATCCTCACC CAGGAAGGAT GGGTAGACGT GATGGACCAA
    ACCTTGAATG CTGTGGGACA CATGTGGGCA CCTGTGGTGG CCATCTATTT CATTTTGTAC
    CATCTCTTTG CCACTCTGAT CCTCCTGAGT TTGTTTGTTG CGGTTATTTT GGACAACTTA
    GAACTTGATG AAGATCTAAA GAAGCTTAAA CAATTAAAAC AAAGTGAAGC GAATGCGGAC
    ACCAAAGAAA AGCTCCCCTT GCGACTTCGA ATCTTTGAAA AATTTCCAAA CAGACCGCAA
    ATGGTGAAAA TCTCAAAGCT TCCTTCAGAT TTTACAGTTC CTAAAATCAG GGAAAGTTTC
    ATGAAGCAGT TTATTGATCG CCAGCAACAG GACACCTGCT GCCTCTTACG AAGCCTTCCG
    TCCACCTCTT CTTCATCGTG TGATCACTCC AAACGGTCTG CAATTGAAGA CAACAAATAC
    ATTGACCAAA AACTTCGCAA GTCTGTTTTC AGCATCAGGG CAAGGAATCT TCTAGAGAAG
    GAGACTGCAG TCACTAAAAT CTTAAGAGCT TGCACTCGAC AGCGCATGCT GAGCGGATCA
    TTTGAGGGGC AGCCAGCAAA GGAAAGGTCA ATTCTCAGTG TGCAGCATCA TATCCGCCAG
    GAGCGCAGGT CACTGAGACA TGGATCCAAC AGCCAGAGGA TCAGCAGGGG CAAATCTCTT
    GAAACCCTGA CTCAAGACCA CTCCAATACA GTGAGATACA GAAATGCACA GAGAGAAGAC
    AGTGAAATCA AGATGATCCA GGAAAAAAAG GAGCAAGCAG AAATGAAAAG GAAAGTGCAG
    GAAGAGGAGC TGAGAGAGAA TCACCCATAC TTCGACAAGC CACTGTTCAT AGTTGGACGG
    GAGCACAGGT TCAGAAACTT CTGCCGGGTG GTGGTCCGGG CACGCTTCAA TGCATCTAAA
    ACAGATCCCG TCACAGGAGC TGTGAAAAAT ACAAAGTACC ATCAACTTTA TGATTTGCTG
    GGCTTGGTGA CTTACCTGGA CTGGGTCATG ATCATCGTAA CCATCTGTTC CTGCATTTCC
    ATGATGTTTG AGTCCCCCTT TCGAAGAGTC ATGCACGCCC CAACTTTGCA GATTGCCGAG
    TACGTGTTTG TGATATTCAT GAGCATCGAG CTTAATCTGA AGATTATGGC AGATGGCTTA
    TTTTTCACTC CAACTGCTGT CATCAGAGAC TTTGGTGGAG TCATGGACAT ATTTATATAT
    CTTGTAAGCT TGATATTTCT TTGTTGGATG CCTCAAAATG TGCCTGCTGA ATCTGGAGCT
    CAGCTTTTAA TGGTTCTGCG GTGCCTGAGA CCTCTGCGAA TATTCAAACT GGTGCCCCAG
    ATGAGGAAAG TTGTTCGAGA ACTTTTCAGT GGCTTCAAGG AAATTTTCTT GGTCTCCATT
    CTTTTGTTGA CATTAATGCT TGTATTTGCA AGCTTTGGAG TTCAGCTTTT TGCGGGGAAA
    CTGGCAAAGT GCAACGATCC CAACATTATT AGAAGGGAAG ATTGTAACGG CATATTCAGA
    ATTAATGTAA GTGTGTCCAA GAACTTAAAT TTAAAATTAA GACCTGGAGA GAAAAAGCCT
    GGATTTTGGG TGCCCCGTGT TTGGGCAAAT CCTAGGAATT TTAATTTTGA CAATGTGGGA
    AATGCTATGC TGGCATTATT TGAAGTGCTG TCCCTGAAAG GCTGGGTGGA AGTGAGGGAT
    GTTATCATTC ATCGTGTGGG GCCGATGCAT GGGATCTATA TTCATGTTTT TGTATTCCTG
    GGTTGCATGA TTGGACTGAC CCTTTTTGTT GGAGTAGTTA TTGCTAATTT CAATGAAAAC
    AAGGGGACAG CTCTGCTGAC TGTAGATCAG AGAAGATGGG AAGATCTGAA GAGCCGATTG
    AAGATAGCCC AGCCTCTTCA TCTCCCACCT CGCCCGGATA ATGATGGTTT TAGAGCTAAG
    ATGTATGACA TCACCCAGCA TCCGTTCTTT AAGAGGACAA TCGCATTGCT TGTTTTGGCC
    CAGTCGGTGC TGCTGTCCGT CAAGTGGGAC GTGGAGGACC CAGTGACTGT CCCTTTGGCA
    ACCATGTCAG TTGTTTTCAC CTTCATCTTT GTTCTAGAGG TTACAATGAA GATCATAGCC
    ATGTCGCCCG CCGGCTTCTG GCAAAGCAGA AGAAACCGAT ACGACCTGCT GGTGACGTCA
    CTTGGTGTGG TCTGGGTGGT ACTTCACTTT GCCCTCCTGA ACGCATATAC CTACATGATG
    GGTGCCTGCG TGATTGTCTT CCGGTTTTTC TCTATCTGTG GAAAGCATGT AACGCTAAAG
    ATGCTCCTCC TGACAGTGGT TGTCAGCATG TATAAAAGCT TCTTCATCAT AGTAGGGATG
    TTCCTTTTGC TCTTGTGTTA TGCTTTTGCT GGAGTTGTTT TATTTGGTAC TGTGAAATAT
    GGAGAGAACA TTAACAGGCA TGCCAACTTT TCTTCAGCTG GCAAAGCCAT CACCGTACTG
    TTCCGAATTG TCACAGGCGA AGACTGGAAT AAGATTATGC ATGACTGTAT GGTCCAGCCT
    CCCTTTTGCA CACCAGATGA ATTCACATAC TGGGCAACAG ACTGTGGAAA TTATGCCGGG
    GCGCTTATGT ATTTCTGTTC ATTTTATGTC ATCATTGCCT ACATCATGCT AAATCTGCTT
    GTAGCCATCA TTGTGGAGAA TTTCTCCTTG TTTTATTCCA CGGAGGAGGA CCAGCTCCTA
    AGTTACAATG ATCTCCGCCA CTTTCAAATC ATATGGAATA TGGTGGATGA TAAACGAGAG
    GGGGTGATTC CAACATTCCG GGTAAAGTTC CTGCTGAGGC TGCTGCGGGG GAGGCTGGAG
    GTGGATCTAG ACAAAGACAA GCTCCTGTTT AAGCACATGT GCTATGAAAT GGAGAGGCTG
    CATAATGGCG GCGATGTCAC TTTCCATGAC GTGCTCAGCA TGCTCTCTTA CCGGTCAGTT
    GACATCCGGA AGAGTTTGCA GTTGGAGGAG CTGCTGGCCA GGGAGCAGCT GGAGTACACC
    ATCGAGGAGG AGGTGGCCAA GCAGACCATC CGCATGTGGC TGAAGAAGTG CTTAAAGCGC
    ATTAGAGCTA AACAGCAGCA GTCCTGCAGC ATCATCCACA GCCTGAGGGA GAGCCAACAG
    CAGGAGCTGA GCCGCTTCCT GAATCCTCCC AGTATCGAGA CCACCCAGCC CAGTGAGGAC
    ATGAACGCTA ACAGTCAGGA TAATAATATG CAACCTGAGA CCAGCAGCCA GCAGCAGCTT
    CTCAGCCCCA CGCTGTCGGA CAGGGGGGGC GGCCGGCAGG ACTCCGCGGA CCCCGGGAAG
    CCCCAGAGGA AGATCGGACA GTGGCGGCTG CCCTCAGGGC GCACAACAAT GAAATCCGTA
    GTGTGCAAAA TGAACCCCAT GACCGACACG GCGTCGTGTG GTTCAGAGGT TAAGAAGTGG
    TGGACGCGGC AGCTGACAGT GGAAAGCGAC GAAAGTGGCG ATGACCTCCT GGATATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe296221
    Clone ID Related Accession (Same CDS sequence) XM_022408132.1
    Accession Version XM_022408132.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5418bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product sodium leak channel non-selective protein isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006604.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAGGAGAC AAGACGACCC CCCAGAGACA CTCAATATCA TGCTTCCTGC TGGCACATCT 
    AGGAACCACA CTGCGACTGC AGTTTTGTAC TTGCTCACCA AAGCTAACTT CAGCATGCTC
    AAAAGGAAGC AGAGCTCCAG GGTGGAGGCC CAGCCAGTTA CTGACTTTGG TCCCGACGAA
    TCTCTGTCCG ACAACGCTGA CATACTCTGG ATTAATAAGC CATGGGTTCA CTCTTTGCTG
    CGCATCTGTG CCATCATCAG TGTCATTTCT GTCTGTATGA ACACACCCAT GACGTTCGAA
    CACTACCCCC CTCTTCAGTA TGTGACTTTT ACTCTGGATA CTTTACTGAT GTTTCTCTAC
    ACAGCAGAGA TGATAGCAAA AATGCACATC CGGGGTATTG TCAAGGGGGA TAGTTCCTAT
    GTGAAAGATC GCTGGTGTGT TTTTGATGGA TTTATGGTCT TCTGCCTTTG GGTTTCATTG
    GTGTTACAGG TATTTGAAAT TGCTGATATA GTTGACCAGA TGTCACCTTG GGGCATGTTG
    CGGATCCCAC GGCCACTAAT TATGATTCGG GCATTCCGGA TTTATTTCCG ATTTGAGCTA
    CCAAGGACCA GAATTACAAA CATTTTAAAG CGATCAGGAG AACAAATATG GAGCGTTTCA
    ATTTTCCTAC TTTTCTTTCT ACTTCTTTAT GGAATTTTAG GAGTTCAGAT GTTTGGAACA
    TTTACCTATC ACTGTGTAGT AAATGACACG AAACCAGGAA ACGTAACCTG GAATAGCTTA
    GCTATCCCAG ATACGCACTG CTCACCAGAG CTGGAGGAGG GCTACCAGTG CCCCCCAGGA
    TTTAAATGCA TGGACCTGGA AGACCTGGGA CTTAGCAGGC AAGAGCTGGG CTACAGTGGC
    TTTAATGAGA TAGGCACCAG CATCTTCACG GTCTATGAGG CCGCGTCCCA GGAAGGCTGG
    GTGTTCCTCA TGTACAGAGC TATTGACAGC TTTCCCCGCT GGCGCTCCTA CTTCTACTTC
    ATCACTCTTA TCTTCTTCCT GGCCTGGCTC GTTAAGAATG TATTTATCGC TGTCATCATT
    GAAACATTTG CAGAAATCAG GGTGCAGTTT CAACAAATGT GGGGATCGAG AAGCAATACT
    ACGTCAACGG CCACGACGCA GATGTTTCAT GAAGACGCTG CCGGCGGATG GCAGCTGGTA
    GCTGTGGATG TCAACAAGCC CCAGGGACGC GCCCCAGCCT GCCTCCAGCA AATGATGCGG
    TCATCAGTTT TCCACATGTT CATTCTGAGC ATGGTGACTG TGGATGTGAT AGTGGCTGCA
    AGCAACTACC ACAAAGGAGA AAGCTTCAGA AGGCAGTACG ATGAGTTCTA TCTTGCAGAG
    GTGGCTTTCA CAGTACTTTT TGATTTGGAA GCACTTCTGA AGATATGGTG TTTAGGATTT
    ACTGGATATA TCAGCTCTTC TCTCCACAAA TTTGAACTAC TACTCGTAAT TGGAACTACT
    CTTCATGTAT ATCCAGATCT TTATCATTCT CAGTTCACAT ACTTTCAGGT TCTTCGGGTA
    GTTCGACTTA TTAAGATCTC ACCTGCACTA GAAGACTTTG TGTACAAGAT ATTTGGTCCT
    GGAAAAAAGC TTGGGAGTTT GGTTGTATTT ACTGCCAGCC TCTTGATTGT TATGTCAGCA
    ATTAGTCTGC AGATGTTCTG CTTTGTCGAA GAACTGGACA GGTTTACAAC GTTTCCAAGG
    GCATTTATGT CCATGTTCCA GATCCTCACC CAGGAAGGAT GGGTAGACGT GATGGACCAA
    ACCTTGAATG CTGTGGGACA CATGTGGGCA CCTGTGGTGG CCATCTATTT CATTTTGTAC
    CATCTCTTTG CCACTCTGAT CCTCCTGAGT TTGTTTGTTG CGGTTATTTT GGACAACTTA
    GAACTTGATG AAGATCTAAA GAAGCTTAAA CAATTAAAAC AAAGTGAAGC GAATGCGGAC
    ACCAAAGAAA AGCTCCCCTT GCGACTTCGA ATCTTTGAAA AATTTCCAAA CAGACCGCAA
    ATGGTGAAAA TCTCAAAGCT TCCTTCAGAT TTTACAGTTC CTAAAATCAG GGAAAGTTTC
    ATGAAGCAGT TTATTGATCG CCAGCAACAG GACACCTGCT GCCTCTTACG AAGCCTTCCG
    TCCACCTCTT CTTCATCGTG TGATCACTCC AAACGGTCTG CAATTGAAGA CAACAAATAC
    ATTGACCAAA AACTTCGCAA GTCTGTTTTC AGCATCAGGG CAAGGAATCT TCTAGAGAAG
    GAGACTGCAG TCACTAAAAT CTTAAGCAAG AGGCCATTAC AGAGGAGCTC ACCTATTAGC
    ATCTGCTGTT GTCTCCCCTT TGGAGGATCT CATTGTCACT TTCCTGGAGT CAGAGCTTGC
    ACTCGACAGC GCATGCTGAG CGGATCATTT GAGGGGCAGC CAGCAAAGGA AAGGTCAATT
    CTCAGTGTGC AGCATCATAT CCGCCAGGAG CGCAGGTCAC TGAGACATGG ATCCAACAGC
    CAGAGGATCA GCAGGGGCAA ATCTCTTGAA ACCCTGACTC AAGACCACTC CAATACAGTG
    AGATACAGAA ATGCACAGAG AGAAGACAGT GAAATCAAGA TGATCCAGGA AAAAAAGGAG
    CAAGCAGAAA TGAAAAGGAA AGTGCAGGAA GAGGAGCTGA GAGAGAATCA CCCATACTTC
    GACAAGCCAC TGTTCATAGT TGGACGGGAG CACAGGTTCA GAAACTTCTG CCGGGTGGTG
    GTCCGGGCAC GCTTCAATGC ATCTAAAACA GATCCCGTCA CAGGAGCTGT GAAAAATACA
    AAGTACCATC AACTTTATGA TTTGCTGGGC TTGGTGACTT ACCTGGACTG GGTCATGATC
    ATCGTAACCA TCTGTTCCTG CATTTCCATG ATGTTTGAGT CCCCCTTTCG AAGAGTCATG
    CACGCCCCAA CTTTGCAGAT TGCCGAGTAC GTGTTTGTGA TATTCATGAG CATCGAGCTT
    AATCTGAAGA TTATGGCAGA TGGCTTATTT TTCACTCCAA CTGCTGTCAT CAGAGACTTT
    GGTGGAGTCA TGGACATATT TATATATCTT GTAAGCTTGA TATTTCTTTG TTGGATGCCT
    CAAAATGTGC CTGCTGAATC TGGAGCTCAG CTTTTAATGG TTCTGCGGTG CCTGAGACCT
    CTGCGAATAT TCAAACTGGT GCCCCAGATG AGGAAAGTTG TTCGAGAACT TTTCAGTGGC
    TTCAAGGAAA TTTTCTTGGT CTCCATTCTT TTGTTGACAT TAATGCTTGT ATTTGCAAGC
    TTTGGAGTTC AGCTTTTTGC GGGGAAACTG GCAAAGTGCA ACGATCCCAA CATTATTAGA
    AGGGAAGATT GTAACGGCAT ATTCAGAATT AATGTAAGTG TGTCCAAGAA CTTAAATTTA
    AAATTAAGAC CTGGAGAGAA AAAGCCTGGA TTTTGGGTGC CCCGTGTTTG GGCAAATCCT
    AGGAATTTTA ATTTTGACAA TGTGGGAAAT GCTATGCTGG CATTATTTGA AGTGCTGTCC
    CTGAAAGGCT GGGTGGAAGT GAGGGATGTT ATCATTCATC GTGTGGGGCC GATGCATGGG
    ATCTATATTC ATGTTTTTGT ATTCCTGGGT TGCATGATTG GACTGACCCT TTTTGTTGGA
    GTAGTTATTG CTAATTTCAA TGAAAACAAG GGGACAGCTC TGCTGACTGT AGATCAGAGA
    AGATGGGAAG ATCTGAAGAG CCGATTGAAG ATAGCCCAGC CTCTTCATCT CCCACCTCGC
    CCGGATAATG ATGGTTTTAG AGCTAAGATG TATGACATCA CCCAGCATCC GTTCTTTAAG
    AGGACAATCG CATTGCTTGT TTTGGCCCAG TCGGTGCTGC TGTCCGTCAA GTGGGACGTG
    GAGGACCCAG TGACTGTCCC TTTGGCAACC ATGTCAGTTG TTTTCACCTT CATCTTTGTT
    CTAGAGGTTA CAATGAAGAT CATAGCCATG TCGCCCGCCG GCTTCTGGCA AAGCAGAAGA
    AACCGATACG ACCTGCTGGT GACGTCACTT GGTGTGGTCT GGGTGGTACT TCACTTTGCC
    CTCCTGAACG CATATACCTA CATGATGGGT GCCTGCGTGA TTGTCTTCCG GTTTTTCTCT
    ATCTGTGGAA AGCATGTAAC GCTAAAGATG CTCCTCCTGA CAGTGGTTGT CAGCATGTAT
    AAAAGCTTCT TCATCATAGT AGGGATGTTC CTTTTGCTCT TGTGTTATGC TTTTGCTGGA
    GTTGTTTTAT TTGGTACTGT GAAATATGGA GAGAACATTA ACAGGCATGC CAACTTTTCT
    TCAGCTGGCA AAGCCATCAC CGTACTGTTC CGAATTGTCA CAGGCGAAGA CTGGAATAAG
    ATTATGCATG ACTGTATGGT CCAGCCTCCC TTTTGCACAC CAGATGAATT CACATACTGG
    GCAACAGACT GTGGAAATTA TGCCGGGGCG CTTATGTATT TCTGTTCATT TTATGTCATC
    ATTGCCTACA TCATGCTAAA TCTGCTTGTA GCCATCATTG TGGAGAATTT CTCCTTGTTT
    TATTCCACGG AGGAGGACCA GCTCCTAAGT TACAATGATC TCCGCCACTT TCAAATCATA
    TGGAATATGG TGGATGATAA ACGAGAGGGG GTGATTCCAA CATTCCGGGT AAAGTTCCTG
    CTGAGGCTGC TGCGGGGGAG GCTGGAGGTG GATCTAGACA AAGACAAGCT CCTGTTTAAG
    CACATGTGCT ATGAAATGGA GAGGCTGCAT AATGGCGGCG ATGTCACTTT CCATGACGTG
    CTCAGCATGC TCTCTTACCG GTCAGTTGAC ATCCGGAAGA GTTTGCAGTT GGAGGAGCTG
    CTGGCCAGGG AGCAGCTGGA GTACACCATC GAGGAGGAGG TGGCCAAGCA GACCATCCGC
    ATGTGGCTGA AGAAGTGCTT AAAGCGCATT AGAGCTAAAC AGCAGCAGTC CTGCAGCATC
    ATCCACAGCC TGAGGGAGAG CCAACAGCAG GAGCTGAGCC GCTTCCTGAA TCCTCCCAGT
    ATCGAGACCA CCCAGCCCAG TGAGGACATG AACGCTAACA GTCAGGATAA TAATATGCAA
    CCTGAGACCA GCAGCCAGCA GCAGCTTCTC AGCCCCACGC TGTCGGACAG GGGGGGCGGC
    CGGCAGGACT CCGCGGACCC CGGGAAGCCC CAGAGGAAGA TCGGACAGTG GCGGCTGCCC
    TCAGCACCAA AACCAATAAG CCACTCAGTG TCTTCGGTCA ACTTACGATT TGCAGGGCGC
    ACAACAATGA AATCCGTAGT GTGCAAAATG AACCCCATGA CCGACACGGC GTCGTGTGGT
    TCAGAGGTTA AGAAGTGGTG GACGCGGCAG CTGACAGTGG AAAGCGACGA AAGTGGCGAT
    GACCTCCTGG ATATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022263840.1
    CDS357..5774
    Translation

    Target ORF information:

    RefSeq Version XM_022408132.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris sodium leak channel, non-selective (NALCN), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022408132.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAGGAGAC AAGACGACCC CCCAGAGACA CTCAATATCA TGCTTCCTGC TGGCACATCT 
    AGGAACCACA CTGCGACTGC AGTTTTGTAC TTGCTCACCA AAGCTAACTT CAGCATGCTC
    AAAAGGAAGC AGAGCTCCAG GGTGGAGGCC CAGCCAGTTA CTGACTTTGG TCCCGACGAA
    TCTCTGTCCG ACAACGCTGA CATACTCTGG ATTAATAAGC CATGGGTTCA CTCTTTGCTG
    CGCATCTGTG CCATCATCAG TGTCATTTCT GTCTGTATGA ACACACCCAT GACGTTCGAA
    CACTACCCCC CTCTTCAGTA TGTGACTTTT ACTCTGGATA CTTTACTGAT GTTTCTCTAC
    ACAGCAGAGA TGATAGCAAA AATGCACATC CGGGGTATTG TCAAGGGGGA TAGTTCCTAT
    GTGAAAGATC GCTGGTGTGT TTTTGATGGA TTTATGGTCT TCTGCCTTTG GGTTTCATTG
    GTGTTACAGG TATTTGAAAT TGCTGATATA GTTGACCAGA TGTCACCTTG GGGCATGTTG
    CGGATCCCAC GGCCACTAAT TATGATTCGG GCATTCCGGA TTTATTTCCG ATTTGAGCTA
    CCAAGGACCA GAATTACAAA CATTTTAAAG CGATCAGGAG AACAAATATG GAGCGTTTCA
    ATTTTCCTAC TTTTCTTTCT ACTTCTTTAT GGAATTTTAG GAGTTCAGAT GTTTGGAACA
    TTTACCTATC ACTGTGTAGT AAATGACACG AAACCAGGAA ACGTAACCTG GAATAGCTTA
    GCTATCCCAG ATACGCACTG CTCACCAGAG CTGGAGGAGG GCTACCAGTG CCCCCCAGGA
    TTTAAATGCA TGGACCTGGA AGACCTGGGA CTTAGCAGGC AAGAGCTGGG CTACAGTGGC
    TTTAATGAGA TAGGCACCAG CATCTTCACG GTCTATGAGG CCGCGTCCCA GGAAGGCTGG
    GTGTTCCTCA TGTACAGAGC TATTGACAGC TTTCCCCGCT GGCGCTCCTA CTTCTACTTC
    ATCACTCTTA TCTTCTTCCT GGCCTGGCTC GTTAAGAATG TATTTATCGC TGTCATCATT
    GAAACATTTG CAGAAATCAG GGTGCAGTTT CAACAAATGT GGGGATCGAG AAGCAATACT
    ACGTCAACGG CCACGACGCA GATGTTTCAT GAAGACGCTG CCGGCGGATG GCAGCTGGTA
    GCTGTGGATG TCAACAAGCC CCAGGGACGC GCCCCAGCCT GCCTCCAGCA AATGATGCGG
    TCATCAGTTT TCCACATGTT CATTCTGAGC ATGGTGACTG TGGATGTGAT AGTGGCTGCA
    AGCAACTACC ACAAAGGAGA AAGCTTCAGA AGGCAGTACG ATGAGTTCTA TCTTGCAGAG
    GTGGCTTTCA CAGTACTTTT TGATTTGGAA GCACTTCTGA AGATATGGTG TTTAGGATTT
    ACTGGATATA TCAGCTCTTC TCTCCACAAA TTTGAACTAC TACTCGTAAT TGGAACTACT
    CTTCATGTAT ATCCAGATCT TTATCATTCT CAGTTCACAT ACTTTCAGGT TCTTCGGGTA
    GTTCGACTTA TTAAGATCTC ACCTGCACTA GAAGACTTTG TGTACAAGAT ATTTGGTCCT
    GGAAAAAAGC TTGGGAGTTT GGTTGTATTT ACTGCCAGCC TCTTGATTGT TATGTCAGCA
    ATTAGTCTGC AGATGTTCTG CTTTGTCGAA GAACTGGACA GGTTTACAAC GTTTCCAAGG
    GCATTTATGT CCATGTTCCA GATCCTCACC CAGGAAGGAT GGGTAGACGT GATGGACCAA
    ACCTTGAATG CTGTGGGACA CATGTGGGCA CCTGTGGTGG CCATCTATTT CATTTTGTAC
    CATCTCTTTG CCACTCTGAT CCTCCTGAGT TTGTTTGTTG CGGTTATTTT GGACAACTTA
    GAACTTGATG AAGATCTAAA GAAGCTTAAA CAATTAAAAC AAAGTGAAGC GAATGCGGAC
    ACCAAAGAAA AGCTCCCCTT GCGACTTCGA ATCTTTGAAA AATTTCCAAA CAGACCGCAA
    ATGGTGAAAA TCTCAAAGCT TCCTTCAGAT TTTACAGTTC CTAAAATCAG GGAAAGTTTC
    ATGAAGCAGT TTATTGATCG CCAGCAACAG GACACCTGCT GCCTCTTACG AAGCCTTCCG
    TCCACCTCTT CTTCATCGTG TGATCACTCC AAACGGTCTG CAATTGAAGA CAACAAATAC
    ATTGACCAAA AACTTCGCAA GTCTGTTTTC AGCATCAGGG CAAGGAATCT TCTAGAGAAG
    GAGACTGCAG TCACTAAAAT CTTAAGCAAG AGGCCATTAC AGAGGAGCTC ACCTATTAGC
    ATCTGCTGTT GTCTCCCCTT TGGAGGATCT CATTGTCACT TTCCTGGAGT CAGAGCTTGC
    ACTCGACAGC GCATGCTGAG CGGATCATTT GAGGGGCAGC CAGCAAAGGA AAGGTCAATT
    CTCAGTGTGC AGCATCATAT CCGCCAGGAG CGCAGGTCAC TGAGACATGG ATCCAACAGC
    CAGAGGATCA GCAGGGGCAA ATCTCTTGAA ACCCTGACTC AAGACCACTC CAATACAGTG
    AGATACAGAA ATGCACAGAG AGAAGACAGT GAAATCAAGA TGATCCAGGA AAAAAAGGAG
    CAAGCAGAAA TGAAAAGGAA AGTGCAGGAA GAGGAGCTGA GAGAGAATCA CCCATACTTC
    GACAAGCCAC TGTTCATAGT TGGACGGGAG CACAGGTTCA GAAACTTCTG CCGGGTGGTG
    GTCCGGGCAC GCTTCAATGC ATCTAAAACA GATCCCGTCA CAGGAGCTGT GAAAAATACA
    AAGTACCATC AACTTTATGA TTTGCTGGGC TTGGTGACTT ACCTGGACTG GGTCATGATC
    ATCGTAACCA TCTGTTCCTG CATTTCCATG ATGTTTGAGT CCCCCTTTCG AAGAGTCATG
    CACGCCCCAA CTTTGCAGAT TGCCGAGTAC GTGTTTGTGA TATTCATGAG CATCGAGCTT
    AATCTGAAGA TTATGGCAGA TGGCTTATTT TTCACTCCAA CTGCTGTCAT CAGAGACTTT
    GGTGGAGTCA TGGACATATT TATATATCTT GTAAGCTTGA TATTTCTTTG TTGGATGCCT
    CAAAATGTGC CTGCTGAATC TGGAGCTCAG CTTTTAATGG TTCTGCGGTG CCTGAGACCT
    CTGCGAATAT TCAAACTGGT GCCCCAGATG AGGAAAGTTG TTCGAGAACT TTTCAGTGGC
    TTCAAGGAAA TTTTCTTGGT CTCCATTCTT TTGTTGACAT TAATGCTTGT ATTTGCAAGC
    TTTGGAGTTC AGCTTTTTGC GGGGAAACTG GCAAAGTGCA ACGATCCCAA CATTATTAGA
    AGGGAAGATT GTAACGGCAT ATTCAGAATT AATGTAAGTG TGTCCAAGAA CTTAAATTTA
    AAATTAAGAC CTGGAGAGAA AAAGCCTGGA TTTTGGGTGC CCCGTGTTTG GGCAAATCCT
    AGGAATTTTA ATTTTGACAA TGTGGGAAAT GCTATGCTGG CATTATTTGA AGTGCTGTCC
    CTGAAAGGCT GGGTGGAAGT GAGGGATGTT ATCATTCATC GTGTGGGGCC GATGCATGGG
    ATCTATATTC ATGTTTTTGT ATTCCTGGGT TGCATGATTG GACTGACCCT TTTTGTTGGA
    GTAGTTATTG CTAATTTCAA TGAAAACAAG GGGACAGCTC TGCTGACTGT AGATCAGAGA
    AGATGGGAAG ATCTGAAGAG CCGATTGAAG ATAGCCCAGC CTCTTCATCT CCCACCTCGC
    CCGGATAATG ATGGTTTTAG AGCTAAGATG TATGACATCA CCCAGCATCC GTTCTTTAAG
    AGGACAATCG CATTGCTTGT TTTGGCCCAG TCGGTGCTGC TGTCCGTCAA GTGGGACGTG
    GAGGACCCAG TGACTGTCCC TTTGGCAACC ATGTCAGTTG TTTTCACCTT CATCTTTGTT
    CTAGAGGTTA CAATGAAGAT CATAGCCATG TCGCCCGCCG GCTTCTGGCA AAGCAGAAGA
    AACCGATACG ACCTGCTGGT GACGTCACTT GGTGTGGTCT GGGTGGTACT TCACTTTGCC
    CTCCTGAACG CATATACCTA CATGATGGGT GCCTGCGTGA TTGTCTTCCG GTTTTTCTCT
    ATCTGTGGAA AGCATGTAAC GCTAAAGATG CTCCTCCTGA CAGTGGTTGT CAGCATGTAT
    AAAAGCTTCT TCATCATAGT AGGGATGTTC CTTTTGCTCT TGTGTTATGC TTTTGCTGGA
    GTTGTTTTAT TTGGTACTGT GAAATATGGA GAGAACATTA ACAGGCATGC CAACTTTTCT
    TCAGCTGGCA AAGCCATCAC CGTACTGTTC CGAATTGTCA CAGGCGAAGA CTGGAATAAG
    ATTATGCATG ACTGTATGGT CCAGCCTCCC TTTTGCACAC CAGATGAATT CACATACTGG
    GCAACAGACT GTGGAAATTA TGCCGGGGCG CTTATGTATT TCTGTTCATT TTATGTCATC
    ATTGCCTACA TCATGCTAAA TCTGCTTGTA GCCATCATTG TGGAGAATTT CTCCTTGTTT
    TATTCCACGG AGGAGGACCA GCTCCTAAGT TACAATGATC TCCGCCACTT TCAAATCATA
    TGGAATATGG TGGATGATAA ACGAGAGGGG GTGATTCCAA CATTCCGGGT AAAGTTCCTG
    CTGAGGCTGC TGCGGGGGAG GCTGGAGGTG GATCTAGACA AAGACAAGCT CCTGTTTAAG
    CACATGTGCT ATGAAATGGA GAGGCTGCAT AATGGCGGCG ATGTCACTTT CCATGACGTG
    CTCAGCATGC TCTCTTACCG GTCAGTTGAC ATCCGGAAGA GTTTGCAGTT GGAGGAGCTG
    CTGGCCAGGG AGCAGCTGGA GTACACCATC GAGGAGGAGG TGGCCAAGCA GACCATCCGC
    ATGTGGCTGA AGAAGTGCTT AAAGCGCATT AGAGCTAAAC AGCAGCAGTC CTGCAGCATC
    ATCCACAGCC TGAGGGAGAG CCAACAGCAG GAGCTGAGCC GCTTCCTGAA TCCTCCCAGT
    ATCGAGACCA CCCAGCCCAG TGAGGACATG AACGCTAACA GTCAGGATAA TAATATGCAA
    CCTGAGACCA GCAGCCAGCA GCAGCTTCTC AGCCCCACGC TGTCGGACAG GGGGGGCGGC
    CGGCAGGACT CCGCGGACCC CGGGAAGCCC CAGAGGAAGA TCGGACAGTG GCGGCTGCCC
    TCAGCACCAA AACCAATAAG CCACTCAGTG TCTTCGGTCA ACTTACGATT TGCAGGGCGC
    ACAACAATGA AATCCGTAGT GTGCAAAATG AACCCCATGA CCGACACGGC GTCGTGTGGT
    TCAGAGGTTA AGAAGTGGTG GACGCGGCAG CTGACAGTGG AAAGCGACGA AAGTGGCGAT
    GACCTCCTGG ATATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe23713
    Clone ID Related Accession (Same CDS sequence) XM_542654.6 , XM_542654.5
    Accession Version XM_542654.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5331bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product sodium leak channel non-selective protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006604.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_542654.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAGGAGAC AAGACGACCC CCCAGAGACA CTCAATATCA TGCTTCCTGC TGGCACATCT 
    AGGAACCACA CTGCGACTGC AGTTTTGTAC TTGCTCACCA AAGCTAACTT CAGCATGCTC
    AAAAGGAAGC AGAGCTCCAG GGTGGAGGCC CAGCCAGTTA CTGACTTTGG TCCCGACGAA
    TCTCTGTCCG ACAACGCTGA CATACTCTGG ATTAATAAGC CATGGGTTCA CTCTTTGCTG
    CGCATCTGTG CCATCATCAG TGTCATTTCT GTCTGTATGA ACACACCCAT GACGTTCGAA
    CACTACCCCC CTCTTCAGTA TGTGACTTTT ACTCTGGATA CTTTACTGAT GTTTCTCTAC
    ACAGCAGAGA TGATAGCAAA AATGCACATC CGGGGTATTG TCAAGGGGGA TAGTTCCTAT
    GTGAAAGATC GCTGGTGTGT TTTTGATGGA TTTATGGTCT TCTGCCTTTG GGTTTCATTG
    GTGTTACAGG TATTTGAAAT TGCTGATATA GTTGACCAGA TGTCACCTTG GGGCATGTTG
    CGGATCCCAC GGCCACTAAT TATGATTCGG GCATTCCGGA TTTATTTCCG ATTTGAGCTA
    CCAAGGACCA GAATTACAAA CATTTTAAAG CGATCAGGAG AACAAATATG GAGCGTTTCA
    ATTTTCCTAC TTTTCTTTCT ACTTCTTTAT GGAATTTTAG GAGTTCAGAT GTTTGGAACA
    TTTACCTATC ACTGTGTAGT AAATGACACG AAACCAGGAA ACGTAACCTG GAATAGCTTA
    GCTATCCCAG ATACGCACTG CTCACCAGAG CTGGAGGAGG GCTACCAGTG CCCCCCAGGA
    TTTAAATGCA TGGACCTGGA AGACCTGGGA CTTAGCAGGC AAGAGCTGGG CTACAGTGGC
    TTTAATGAGA TAGGCACCAG CATCTTCACG GTCTATGAGG CCGCGTCCCA GGAAGGCTGG
    GTGTTCCTCA TGTACAGAGC TATTGACAGC TTTCCCCGCT GGCGCTCCTA CTTCTACTTC
    ATCACTCTTA TCTTCTTCCT GGCCTGGCTC GTTAAGAATG TATTTATCGC TGTCATCATT
    GAAACATTTG CAGAAATCAG GGTGCAGTTT CAACAAATGT GGGGATCGAG AAGCAATACT
    ACGTCAACGG CCACGACGCA GATGTTTCAT GAAGACGCTG CCGGCGGATG GCAGCTGGTA
    GCTGTGGATG TCAACAAGCC CCAGGGACGC GCCCCAGCCT GCCTCCAGCA AATGATGCGG
    TCATCAGTTT TCCACATGTT CATTCTGAGC ATGGTGACTG TGGATGTGAT AGTGGCTGCA
    AGCAACTACC ACAAAGGAGA AAGCTTCAGA AGGCAGTACG ATGAGTTCTA TCTTGCAGAG
    GTGGCTTTCA CAGTACTTTT TGATTTGGAA GCACTTCTGA AGATATGGTG TTTAGGATTT
    ACTGGATATA TCAGCTCTTC TCTCCACAAA TTTGAACTAC TACTCGTAAT TGGAACTACT
    CTTCATGTAT ATCCAGATCT TTATCATTCT CAGTTCACAT ACTTTCAGGT TCTTCGGGTA
    GTTCGACTTA TTAAGATCTC ACCTGCACTA GAAGACTTTG TGTACAAGAT ATTTGGTCCT
    GGAAAAAAGC TTGGGAGTTT GGTTGTATTT ACTGCCAGCC TCTTGATTGT TATGTCAGCA
    ATTAGTCTGC AGATGTTCTG CTTTGTCGAA GAACTGGACA GGTTTACAAC GTTTCCAAGG
    GCATTTATGT CCATGTTCCA GATCCTCACC CAGGAAGGAT GGGTAGACGT GATGGACCAA
    ACCTTGAATG CTGTGGGACA CATGTGGGCA CCTGTGGTGG CCATCTATTT CATTTTGTAC
    CATCTCTTTG CCACTCTGAT CCTCCTGAGT TTGTTTGTTG CGGTTATTTT GGACAACTTA
    GAACTTGATG AAGATCTAAA GAAGCTTAAA CAATTAAAAC AAAGTGAAGC GAATGCGGAC
    ACCAAAGAAA AGCTCCCCTT GCGACTTCGA ATCTTTGAAA AATTTCCAAA CAGACCGCAA
    ATGGTGAAAA TCTCAAAGCT TCCTTCAGAT TTTACAGTTC CTAAAATCAG GGAAAGTTTC
    ATGAAGCAGT TTATTGATCG CCAGCAACAG GACACCTGCT GCCTCTTACG AAGCCTTCCG
    TCCACCTCTT CTTCATCGTG TGATCACTCC AAACGGTCTG CAATTGAAGA CAACAAATAC
    ATTGACCAAA AACTTCGCAA GTCTGTTTTC AGCATCAGGG CAAGGAATCT TCTAGAGAAG
    GAGACTGCAG TCACTAAAAT CTTAAGAGCT TGCACTCGAC AGCGCATGCT GAGCGGATCA
    TTTGAGGGGC AGCCAGCAAA GGAAAGGTCA ATTCTCAGTG TGCAGCATCA TATCCGCCAG
    GAGCGCAGGT CACTGAGACA TGGATCCAAC AGCCAGAGGA TCAGCAGGGG CAAATCTCTT
    GAAACCCTGA CTCAAGACCA CTCCAATACA GTGAGATACA GAAATGCACA GAGAGAAGAC
    AGTGAAATCA AGATGATCCA GGAAAAAAAG GAGCAAGCAG AAATGAAAAG GAAAGTGCAG
    GAAGAGGAGC TGAGAGAGAA TCACCCATAC TTCGACAAGC CACTGTTCAT AGTTGGACGG
    GAGCACAGGT TCAGAAACTT CTGCCGGGTG GTGGTCCGGG CACGCTTCAA TGCATCTAAA
    ACAGATCCCG TCACAGGAGC TGTGAAAAAT ACAAAGTACC ATCAACTTTA TGATTTGCTG
    GGCTTGGTGA CTTACCTGGA CTGGGTCATG ATCATCGTAA CCATCTGTTC CTGCATTTCC
    ATGATGTTTG AGTCCCCCTT TCGAAGAGTC ATGCACGCCC CAACTTTGCA GATTGCCGAG
    TACGTGTTTG TGATATTCAT GAGCATCGAG CTTAATCTGA AGATTATGGC AGATGGCTTA
    TTTTTCACTC CAACTGCTGT CATCAGAGAC TTTGGTGGAG TCATGGACAT ATTTATATAT
    CTTGTAAGCT TGATATTTCT TTGTTGGATG CCTCAAAATG TGCCTGCTGA ATCTGGAGCT
    CAGCTTTTAA TGGTTCTGCG GTGCCTGAGA CCTCTGCGAA TATTCAAACT GGTGCCCCAG
    ATGAGGAAAG TTGTTCGAGA ACTTTTCAGT GGCTTCAAGG AAATTTTCTT GGTCTCCATT
    CTTTTGTTGA CATTAATGCT TGTATTTGCA AGCTTTGGAG TTCAGCTTTT TGCGGGGAAA
    CTGGCAAAGT GCAACGATCC CAACATTATT AGAAGGGAAG ATTGTAACGG CATATTCAGA
    ATTAATGTAA GTGTGTCCAA GAACTTAAAT TTAAAATTAA GACCTGGAGA GAAAAAGCCT
    GGATTTTGGG TGCCCCGTGT TTGGGCAAAT CCTAGGAATT TTAATTTTGA CAATGTGGGA
    AATGCTATGC TGGCATTATT TGAAGTGCTG TCCCTGAAAG GCTGGGTGGA AGTGAGGGAT
    GTTATCATTC ATCGTGTGGG GCCGATGCAT GGGATCTATA TTCATGTTTT TGTATTCCTG
    GGTTGCATGA TTGGACTGAC CCTTTTTGTT GGAGTAGTTA TTGCTAATTT CAATGAAAAC
    AAGGGGACAG CTCTGCTGAC TGTAGATCAG AGAAGATGGG AAGATCTGAA GAGCCGATTG
    AAGATAGCCC AGCCTCTTCA TCTCCCACCT CGCCCGGATA ATGATGGTTT TAGAGCTAAG
    ATGTATGACA TCACCCAGCA TCCGTTCTTT AAGAGGACAA TCGCATTGCT TGTTTTGGCC
    CAGTCGGTGC TGCTGTCCGT CAAGTGGGAC GTGGAGGACC CAGTGACTGT CCCTTTGGCA
    ACCATGTCAG TTGTTTTCAC CTTCATCTTT GTTCTAGAGG TTACAATGAA GATCATAGCC
    ATGTCGCCCG CCGGCTTCTG GCAAAGCAGA AGAAACCGAT ACGACCTGCT GGTGACGTCA
    CTTGGTGTGG TCTGGGTGGT ACTTCACTTT GCCCTCCTGA ACGCATATAC CTACATGATG
    GGTGCCTGCG TGATTGTCTT CCGGTTTTTC TCTATCTGTG GAAAGCATGT AACGCTAAAG
    ATGCTCCTCC TGACAGTGGT TGTCAGCATG TATAAAAGCT TCTTCATCAT AGTAGGGATG
    TTCCTTTTGC TCTTGTGTTA TGCTTTTGCT GGAGTTGTTT TATTTGGTAC TGTGAAATAT
    GGAGAGAACA TTAACAGGCA TGCCAACTTT TCTTCAGCTG GCAAAGCCAT CACCGTACTG
    TTCCGAATTG TCACAGGCGA AGACTGGAAT AAGATTATGC ATGACTGTAT GGTCCAGCCT
    CCCTTTTGCA CACCAGATGA ATTCACATAC TGGGCAACAG ACTGTGGAAA TTATGCCGGG
    GCGCTTATGT ATTTCTGTTC ATTTTATGTC ATCATTGCCT ACATCATGCT AAATCTGCTT
    GTAGCCATCA TTGTGGAGAA TTTCTCCTTG TTTTATTCCA CGGAGGAGGA CCAGCTCCTA
    AGTTACAATG ATCTCCGCCA CTTTCAAATC ATATGGAATA TGGTGGATGA TAAACGAGAG
    GGGGTGATTC CAACATTCCG GGTAAAGTTC CTGCTGAGGC TGCTGCGGGG GAGGCTGGAG
    GTGGATCTAG ACAAAGACAA GCTCCTGTTT AAGCACATGT GCTATGAAAT GGAGAGGCTG
    CATAATGGCG GCGATGTCAC TTTCCATGAC GTGCTCAGCA TGCTCTCTTA CCGGTCAGTT
    GACATCCGGA AGAGTTTGCA GTTGGAGGAG CTGCTGGCCA GGGAGCAGCT GGAGTACACC
    ATCGAGGAGG AGGTGGCCAA GCAGACCATC CGCATGTGGC TGAAGAAGTG CTTAAAGCGC
    ATTAGAGCTA AACAGCAGCA GTCCTGCAGC ATCATCCACA GCCTGAGGGA GAGCCAACAG
    CAGGAGCTGA GCCGCTTCCT GAATCCTCCC AGTATCGAGA CCACCCAGCC CAGTGAGGAC
    ATGAACGCTA ACAGTCAGGA TAATAATATG CAACCTGAGA CCAGCAGCCA GCAGCAGCTT
    CTCAGCCCCA CGCTGTCGGA CAGGGGGGGC GGCCGGCAGG ACTCCGCGGA CCCCGGGAAG
    CCCCAGAGGA AGATCGGACA GTGGCGGCTG CCCTCAGCAC CAAAACCAAT AAGCCACTCA
    GTGTCTTCGG TCAACTTACG ATTTGCAGGG CGCACAACAA TGAAATCCGT AGTGTGCAAA
    ATGAACCCCA TGACCGACAC GGCGTCGTGT GGTTCAGAGG TTAAGAAGTG GTGGACGCGG
    CAGCTGACAG TGGAAAGCGA CGAAAGTGGC GATGACCTCC TGGATATTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_542654.4
    CDS357..5687
    Translation

    Target ORF information:

    RefSeq Version XM_542654.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris sodium leak channel, non-selective (NALCN), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_542654.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAGGAGAC AAGACGACCC CCCAGAGACA CTCAATATCA TGCTTCCTGC TGGCACATCT 
    AGGAACCACA CTGCGACTGC AGTTTTGTAC TTGCTCACCA AAGCTAACTT CAGCATGCTC
    AAAAGGAAGC AGAGCTCCAG GGTGGAGGCC CAGCCAGTTA CTGACTTTGG TCCCGACGAA
    TCTCTGTCCG ACAACGCTGA CATACTCTGG ATTAATAAGC CATGGGTTCA CTCTTTGCTG
    CGCATCTGTG CCATCATCAG TGTCATTTCT GTCTGTATGA ACACACCCAT GACGTTCGAA
    CACTACCCCC CTCTTCAGTA TGTGACTTTT ACTCTGGATA CTTTACTGAT GTTTCTCTAC
    ACAGCAGAGA TGATAGCAAA AATGCACATC CGGGGTATTG TCAAGGGGGA TAGTTCCTAT
    GTGAAAGATC GCTGGTGTGT TTTTGATGGA TTTATGGTCT TCTGCCTTTG GGTTTCATTG
    GTGTTACAGG TATTTGAAAT TGCTGATATA GTTGACCAGA TGTCACCTTG GGGCATGTTG
    CGGATCCCAC GGCCACTAAT TATGATTCGG GCATTCCGGA TTTATTTCCG ATTTGAGCTA
    CCAAGGACCA GAATTACAAA CATTTTAAAG CGATCAGGAG AACAAATATG GAGCGTTTCA
    ATTTTCCTAC TTTTCTTTCT ACTTCTTTAT GGAATTTTAG GAGTTCAGAT GTTTGGAACA
    TTTACCTATC ACTGTGTAGT AAATGACACG AAACCAGGAA ACGTAACCTG GAATAGCTTA
    GCTATCCCAG ATACGCACTG CTCACCAGAG CTGGAGGAGG GCTACCAGTG CCCCCCAGGA
    TTTAAATGCA TGGACCTGGA AGACCTGGGA CTTAGCAGGC AAGAGCTGGG CTACAGTGGC
    TTTAATGAGA TAGGCACCAG CATCTTCACG GTCTATGAGG CCGCGTCCCA GGAAGGCTGG
    GTGTTCCTCA TGTACAGAGC TATTGACAGC TTTCCCCGCT GGCGCTCCTA CTTCTACTTC
    ATCACTCTTA TCTTCTTCCT GGCCTGGCTC GTTAAGAATG TATTTATCGC TGTCATCATT
    GAAACATTTG CAGAAATCAG GGTGCAGTTT CAACAAATGT GGGGATCGAG AAGCAATACT
    ACGTCAACGG CCACGACGCA GATGTTTCAT GAAGACGCTG CCGGCGGATG GCAGCTGGTA
    GCTGTGGATG TCAACAAGCC CCAGGGACGC GCCCCAGCCT GCCTCCAGCA AATGATGCGG
    TCATCAGTTT TCCACATGTT CATTCTGAGC ATGGTGACTG TGGATGTGAT AGTGGCTGCA
    AGCAACTACC ACAAAGGAGA AAGCTTCAGA AGGCAGTACG ATGAGTTCTA TCTTGCAGAG
    GTGGCTTTCA CAGTACTTTT TGATTTGGAA GCACTTCTGA AGATATGGTG TTTAGGATTT
    ACTGGATATA TCAGCTCTTC TCTCCACAAA TTTGAACTAC TACTCGTAAT TGGAACTACT
    CTTCATGTAT ATCCAGATCT TTATCATTCT CAGTTCACAT ACTTTCAGGT TCTTCGGGTA
    GTTCGACTTA TTAAGATCTC ACCTGCACTA GAAGACTTTG TGTACAAGAT ATTTGGTCCT
    GGAAAAAAGC TTGGGAGTTT GGTTGTATTT ACTGCCAGCC TCTTGATTGT TATGTCAGCA
    ATTAGTCTGC AGATGTTCTG CTTTGTCGAA GAACTGGACA GGTTTACAAC GTTTCCAAGG
    GCATTTATGT CCATGTTCCA GATCCTCACC CAGGAAGGAT GGGTAGACGT GATGGACCAA
    ACCTTGAATG CTGTGGGACA CATGTGGGCA CCTGTGGTGG CCATCTATTT CATTTTGTAC
    CATCTCTTTG CCACTCTGAT CCTCCTGAGT TTGTTTGTTG CGGTTATTTT GGACAACTTA
    GAACTTGATG AAGATCTAAA GAAGCTTAAA CAATTAAAAC AAAGTGAAGC GAATGCGGAC
    ACCAAAGAAA AGCTCCCCTT GCGACTTCGA ATCTTTGAAA AATTTCCAAA CAGACCGCAA
    ATGGTGAAAA TCTCAAAGCT TCCTTCAGAT TTTACAGTTC CTAAAATCAG GGAAAGTTTC
    ATGAAGCAGT TTATTGATCG CCAGCAACAG GACACCTGCT GCCTCTTACG AAGCCTTCCG
    TCCACCTCTT CTTCATCGTG TGATCACTCC AAACGGTCTG CAATTGAAGA CAACAAATAC
    ATTGACCAAA AACTTCGCAA GTCTGTTTTC AGCATCAGGG CAAGGAATCT TCTAGAGAAG
    GAGACTGCAG TCACTAAAAT CTTAAGAGCT TGCACTCGAC AGCGCATGCT GAGCGGATCA
    TTTGAGGGGC AGCCAGCAAA GGAAAGGTCA ATTCTCAGTG TGCAGCATCA TATCCGCCAG
    GAGCGCAGGT CACTGAGACA TGGATCCAAC AGCCAGAGGA TCAGCAGGGG CAAATCTCTT
    GAAACCCTGA CTCAAGACCA CTCCAATACA GTGAGATACA GAAATGCACA GAGAGAAGAC
    AGTGAAATCA AGATGATCCA GGAAAAAAAG GAGCAAGCAG AAATGAAAAG GAAAGTGCAG
    GAAGAGGAGC TGAGAGAGAA TCACCCATAC TTCGACAAGC CACTGTTCAT AGTTGGACGG
    GAGCACAGGT TCAGAAACTT CTGCCGGGTG GTGGTCCGGG CACGCTTCAA TGCATCTAAA
    ACAGATCCCG TCACAGGAGC TGTGAAAAAT ACAAAGTACC ATCAACTTTA TGATTTGCTG
    GGCTTGGTGA CTTACCTGGA CTGGGTCATG ATCATCGTAA CCATCTGTTC CTGCATTTCC
    ATGATGTTTG AGTCCCCCTT TCGAAGAGTC ATGCACGCCC CAACTTTGCA GATTGCCGAG
    TACGTGTTTG TGATATTCAT GAGCATCGAG CTTAATCTGA AGATTATGGC AGATGGCTTA
    TTTTTCACTC CAACTGCTGT CATCAGAGAC TTTGGTGGAG TCATGGACAT ATTTATATAT
    CTTGTAAGCT TGATATTTCT TTGTTGGATG CCTCAAAATG TGCCTGCTGA ATCTGGAGCT
    CAGCTTTTAA TGGTTCTGCG GTGCCTGAGA CCTCTGCGAA TATTCAAACT GGTGCCCCAG
    ATGAGGAAAG TTGTTCGAGA ACTTTTCAGT GGCTTCAAGG AAATTTTCTT GGTCTCCATT
    CTTTTGTTGA CATTAATGCT TGTATTTGCA AGCTTTGGAG TTCAGCTTTT TGCGGGGAAA
    CTGGCAAAGT GCAACGATCC CAACATTATT AGAAGGGAAG ATTGTAACGG CATATTCAGA
    ATTAATGTAA GTGTGTCCAA GAACTTAAAT TTAAAATTAA GACCTGGAGA GAAAAAGCCT
    GGATTTTGGG TGCCCCGTGT TTGGGCAAAT CCTAGGAATT TTAATTTTGA CAATGTGGGA
    AATGCTATGC TGGCATTATT TGAAGTGCTG TCCCTGAAAG GCTGGGTGGA AGTGAGGGAT
    GTTATCATTC ATCGTGTGGG GCCGATGCAT GGGATCTATA TTCATGTTTT TGTATTCCTG
    GGTTGCATGA TTGGACTGAC CCTTTTTGTT GGAGTAGTTA TTGCTAATTT CAATGAAAAC
    AAGGGGACAG CTCTGCTGAC TGTAGATCAG AGAAGATGGG AAGATCTGAA GAGCCGATTG
    AAGATAGCCC AGCCTCTTCA TCTCCCACCT CGCCCGGATA ATGATGGTTT TAGAGCTAAG
    ATGTATGACA TCACCCAGCA TCCGTTCTTT AAGAGGACAA TCGCATTGCT TGTTTTGGCC
    CAGTCGGTGC TGCTGTCCGT CAAGTGGGAC GTGGAGGACC CAGTGACTGT CCCTTTGGCA
    ACCATGTCAG TTGTTTTCAC CTTCATCTTT GTTCTAGAGG TTACAATGAA GATCATAGCC
    ATGTCGCCCG CCGGCTTCTG GCAAAGCAGA AGAAACCGAT ACGACCTGCT GGTGACGTCA
    CTTGGTGTGG TCTGGGTGGT ACTTCACTTT GCCCTCCTGA ACGCATATAC CTACATGATG
    GGTGCCTGCG TGATTGTCTT CCGGTTTTTC TCTATCTGTG GAAAGCATGT AACGCTAAAG
    ATGCTCCTCC TGACAGTGGT TGTCAGCATG TATAAAAGCT TCTTCATCAT AGTAGGGATG
    TTCCTTTTGC TCTTGTGTTA TGCTTTTGCT GGAGTTGTTT TATTTGGTAC TGTGAAATAT
    GGAGAGAACA TTAACAGGCA TGCCAACTTT TCTTCAGCTG GCAAAGCCAT CACCGTACTG
    TTCCGAATTG TCACAGGCGA AGACTGGAAT AAGATTATGC ATGACTGTAT GGTCCAGCCT
    CCCTTTTGCA CACCAGATGA ATTCACATAC TGGGCAACAG ACTGTGGAAA TTATGCCGGG
    GCGCTTATGT ATTTCTGTTC ATTTTATGTC ATCATTGCCT ACATCATGCT AAATCTGCTT
    GTAGCCATCA TTGTGGAGAA TTTCTCCTTG TTTTATTCCA CGGAGGAGGA CCAGCTCCTA
    AGTTACAATG ATCTCCGCCA CTTTCAAATC ATATGGAATA TGGTGGATGA TAAACGAGAG
    GGGGTGATTC CAACATTCCG GGTAAAGTTC CTGCTGAGGC TGCTGCGGGG GAGGCTGGAG
    GTGGATCTAG ACAAAGACAA GCTCCTGTTT AAGCACATGT GCTATGAAAT GGAGAGGCTG
    CATAATGGCG GCGATGTCAC TTTCCATGAC GTGCTCAGCA TGCTCTCTTA CCGGTCAGTT
    GACATCCGGA AGAGTTTGCA GTTGGAGGAG CTGCTGGCCA GGGAGCAGCT GGAGTACACC
    ATCGAGGAGG AGGTGGCCAA GCAGACCATC CGCATGTGGC TGAAGAAGTG CTTAAAGCGC
    ATTAGAGCTA AACAGCAGCA GTCCTGCAGC ATCATCCACA GCCTGAGGGA GAGCCAACAG
    CAGGAGCTGA GCCGCTTCCT GAATCCTCCC AGTATCGAGA CCACCCAGCC CAGTGAGGAC
    ATGAACGCTA ACAGTCAGGA TAATAATATG CAACCTGAGA CCAGCAGCCA GCAGCAGCTT
    CTCAGCCCCA CGCTGTCGGA CAGGGGGGGC GGCCGGCAGG ACTCCGCGGA CCCCGGGAAG
    CCCCAGAGGA AGATCGGACA GTGGCGGCTG CCCTCAGCAC CAAAACCAAT AAGCCACTCA
    GTGTCTTCGG TCAACTTACG ATTTGCAGGG CGCACAACAA TGAAATCCGT AGTGTGCAAA
    ATGAACCCCA TGACCGACAC GGCGTCGTGT GGTTCAGAGG TTAAGAAGTG GTGGACGCGG
    CAGCTGACAG TGGAAAGCGA CGAAAGTGGC GATGACCTCC TGGATATTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe23713
    Clone ID Related Accession (Same CDS sequence) XM_542654.6 , XM_542654.5
    Accession Version XM_542654.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5331bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product sodium leak channel non-selective protein isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726098.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_542654.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAGGAGAC AAGACGACCC CCCAGAGACA CTCAATATCA TGCTTCCTGC TGGCACATCT 
    AGGAACCACA CTGCGACTGC AGTTTTGTAC TTGCTCACCA AAGCTAACTT CAGCATGCTC
    AAAAGGAAGC AGAGCTCCAG GGTGGAGGCC CAGCCAGTTA CTGACTTTGG TCCCGACGAA
    TCTCTGTCCG ACAACGCTGA CATACTCTGG ATTAATAAGC CATGGGTTCA CTCTTTGCTG
    CGCATCTGTG CCATCATCAG TGTCATTTCT GTCTGTATGA ACACACCCAT GACGTTCGAA
    CACTACCCCC CTCTTCAGTA TGTGACTTTT ACTCTGGATA CTTTACTGAT GTTTCTCTAC
    ACAGCAGAGA TGATAGCAAA AATGCACATC CGGGGTATTG TCAAGGGGGA TAGTTCCTAT
    GTGAAAGATC GCTGGTGTGT TTTTGATGGA TTTATGGTCT TCTGCCTTTG GGTTTCATTG
    GTGTTACAGG TATTTGAAAT TGCTGATATA GTTGACCAGA TGTCACCTTG GGGCATGTTG
    CGGATCCCAC GGCCACTAAT TATGATTCGG GCATTCCGGA TTTATTTCCG ATTTGAGCTA
    CCAAGGACCA GAATTACAAA CATTTTAAAG CGATCAGGAG AACAAATATG GAGCGTTTCA
    ATTTTCCTAC TTTTCTTTCT ACTTCTTTAT GGAATTTTAG GAGTTCAGAT GTTTGGAACA
    TTTACCTATC ACTGTGTAGT AAATGACACG AAACCAGGAA ACGTAACCTG GAATAGCTTA
    GCTATCCCAG ATACGCACTG CTCACCAGAG CTGGAGGAGG GCTACCAGTG CCCCCCAGGA
    TTTAAATGCA TGGACCTGGA AGACCTGGGA CTTAGCAGGC AAGAGCTGGG CTACAGTGGC
    TTTAATGAGA TAGGCACCAG CATCTTCACG GTCTATGAGG CCGCGTCCCA GGAAGGCTGG
    GTGTTCCTCA TGTACAGAGC TATTGACAGC TTTCCCCGCT GGCGCTCCTA CTTCTACTTC
    ATCACTCTTA TCTTCTTCCT GGCCTGGCTC GTTAAGAATG TATTTATCGC TGTCATCATT
    GAAACATTTG CAGAAATCAG GGTGCAGTTT CAACAAATGT GGGGATCGAG AAGCAATACT
    ACGTCAACGG CCACGACGCA GATGTTTCAT GAAGACGCTG CCGGCGGATG GCAGCTGGTA
    GCTGTGGATG TCAACAAGCC CCAGGGACGC GCCCCAGCCT GCCTCCAGCA AATGATGCGG
    TCATCAGTTT TCCACATGTT CATTCTGAGC ATGGTGACTG TGGATGTGAT AGTGGCTGCA
    AGCAACTACC ACAAAGGAGA AAGCTTCAGA AGGCAGTACG ATGAGTTCTA TCTTGCAGAG
    GTGGCTTTCA CAGTACTTTT TGATTTGGAA GCACTTCTGA AGATATGGTG TTTAGGATTT
    ACTGGATATA TCAGCTCTTC TCTCCACAAA TTTGAACTAC TACTCGTAAT TGGAACTACT
    CTTCATGTAT ATCCAGATCT TTATCATTCT CAGTTCACAT ACTTTCAGGT TCTTCGGGTA
    GTTCGACTTA TTAAGATCTC ACCTGCACTA GAAGACTTTG TGTACAAGAT ATTTGGTCCT
    GGAAAAAAGC TTGGGAGTTT GGTTGTATTT ACTGCCAGCC TCTTGATTGT TATGTCAGCA
    ATTAGTCTGC AGATGTTCTG CTTTGTCGAA GAACTGGACA GGTTTACAAC GTTTCCAAGG
    GCATTTATGT CCATGTTCCA GATCCTCACC CAGGAAGGAT GGGTAGACGT GATGGACCAA
    ACCTTGAATG CTGTGGGACA CATGTGGGCA CCTGTGGTGG CCATCTATTT CATTTTGTAC
    CATCTCTTTG CCACTCTGAT CCTCCTGAGT TTGTTTGTTG CGGTTATTTT GGACAACTTA
    GAACTTGATG AAGATCTAAA GAAGCTTAAA CAATTAAAAC AAAGTGAAGC GAATGCGGAC
    ACCAAAGAAA AGCTCCCCTT GCGACTTCGA ATCTTTGAAA AATTTCCAAA CAGACCGCAA
    ATGGTGAAAA TCTCAAAGCT TCCTTCAGAT TTTACAGTTC CTAAAATCAG GGAAAGTTTC
    ATGAAGCAGT TTATTGATCG CCAGCAACAG GACACCTGCT GCCTCTTACG AAGCCTTCCG
    TCCACCTCTT CTTCATCGTG TGATCACTCC AAACGGTCTG CAATTGAAGA CAACAAATAC
    ATTGACCAAA AACTTCGCAA GTCTGTTTTC AGCATCAGGG CAAGGAATCT TCTAGAGAAG
    GAGACTGCAG TCACTAAAAT CTTAAGAGCT TGCACTCGAC AGCGCATGCT GAGCGGATCA
    TTTGAGGGGC AGCCAGCAAA GGAAAGGTCA ATTCTCAGTG TGCAGCATCA TATCCGCCAG
    GAGCGCAGGT CACTGAGACA TGGATCCAAC AGCCAGAGGA TCAGCAGGGG CAAATCTCTT
    GAAACCCTGA CTCAAGACCA CTCCAATACA GTGAGATACA GAAATGCACA GAGAGAAGAC
    AGTGAAATCA AGATGATCCA GGAAAAAAAG GAGCAAGCAG AAATGAAAAG GAAAGTGCAG
    GAAGAGGAGC TGAGAGAGAA TCACCCATAC TTCGACAAGC CACTGTTCAT AGTTGGACGG
    GAGCACAGGT TCAGAAACTT CTGCCGGGTG GTGGTCCGGG CACGCTTCAA TGCATCTAAA
    ACAGATCCCG TCACAGGAGC TGTGAAAAAT ACAAAGTACC ATCAACTTTA TGATTTGCTG
    GGCTTGGTGA CTTACCTGGA CTGGGTCATG ATCATCGTAA CCATCTGTTC CTGCATTTCC
    ATGATGTTTG AGTCCCCCTT TCGAAGAGTC ATGCACGCCC CAACTTTGCA GATTGCCGAG
    TACGTGTTTG TGATATTCAT GAGCATCGAG CTTAATCTGA AGATTATGGC AGATGGCTTA
    TTTTTCACTC CAACTGCTGT CATCAGAGAC TTTGGTGGAG TCATGGACAT ATTTATATAT
    CTTGTAAGCT TGATATTTCT TTGTTGGATG CCTCAAAATG TGCCTGCTGA ATCTGGAGCT
    CAGCTTTTAA TGGTTCTGCG GTGCCTGAGA CCTCTGCGAA TATTCAAACT GGTGCCCCAG
    ATGAGGAAAG TTGTTCGAGA ACTTTTCAGT GGCTTCAAGG AAATTTTCTT GGTCTCCATT
    CTTTTGTTGA CATTAATGCT TGTATTTGCA AGCTTTGGAG TTCAGCTTTT TGCGGGGAAA
    CTGGCAAAGT GCAACGATCC CAACATTATT AGAAGGGAAG ATTGTAACGG CATATTCAGA
    ATTAATGTAA GTGTGTCCAA GAACTTAAAT TTAAAATTAA GACCTGGAGA GAAAAAGCCT
    GGATTTTGGG TGCCCCGTGT TTGGGCAAAT CCTAGGAATT TTAATTTTGA CAATGTGGGA
    AATGCTATGC TGGCATTATT TGAAGTGCTG TCCCTGAAAG GCTGGGTGGA AGTGAGGGAT
    GTTATCATTC ATCGTGTGGG GCCGATGCAT GGGATCTATA TTCATGTTTT TGTATTCCTG
    GGTTGCATGA TTGGACTGAC CCTTTTTGTT GGAGTAGTTA TTGCTAATTT CAATGAAAAC
    AAGGGGACAG CTCTGCTGAC TGTAGATCAG AGAAGATGGG AAGATCTGAA GAGCCGATTG
    AAGATAGCCC AGCCTCTTCA TCTCCCACCT CGCCCGGATA ATGATGGTTT TAGAGCTAAG
    ATGTATGACA TCACCCAGCA TCCGTTCTTT AAGAGGACAA TCGCATTGCT TGTTTTGGCC
    CAGTCGGTGC TGCTGTCCGT CAAGTGGGAC GTGGAGGACC CAGTGACTGT CCCTTTGGCA
    ACCATGTCAG TTGTTTTCAC CTTCATCTTT GTTCTAGAGG TTACAATGAA GATCATAGCC
    ATGTCGCCCG CCGGCTTCTG GCAAAGCAGA AGAAACCGAT ACGACCTGCT GGTGACGTCA
    CTTGGTGTGG TCTGGGTGGT ACTTCACTTT GCCCTCCTGA ACGCATATAC CTACATGATG
    GGTGCCTGCG TGATTGTCTT CCGGTTTTTC TCTATCTGTG GAAAGCATGT AACGCTAAAG
    ATGCTCCTCC TGACAGTGGT TGTCAGCATG TATAAAAGCT TCTTCATCAT AGTAGGGATG
    TTCCTTTTGC TCTTGTGTTA TGCTTTTGCT GGAGTTGTTT TATTTGGTAC TGTGAAATAT
    GGAGAGAACA TTAACAGGCA TGCCAACTTT TCTTCAGCTG GCAAAGCCAT CACCGTACTG
    TTCCGAATTG TCACAGGCGA AGACTGGAAT AAGATTATGC ATGACTGTAT GGTCCAGCCT
    CCCTTTTGCA CACCAGATGA ATTCACATAC TGGGCAACAG ACTGTGGAAA TTATGCCGGG
    GCGCTTATGT ATTTCTGTTC ATTTTATGTC ATCATTGCCT ACATCATGCT AAATCTGCTT
    GTAGCCATCA TTGTGGAGAA TTTCTCCTTG TTTTATTCCA CGGAGGAGGA CCAGCTCCTA
    AGTTACAATG ATCTCCGCCA CTTTCAAATC ATATGGAATA TGGTGGATGA TAAACGAGAG
    GGGGTGATTC CAACATTCCG GGTAAAGTTC CTGCTGAGGC TGCTGCGGGG GAGGCTGGAG
    GTGGATCTAG ACAAAGACAA GCTCCTGTTT AAGCACATGT GCTATGAAAT GGAGAGGCTG
    CATAATGGCG GCGATGTCAC TTTCCATGAC GTGCTCAGCA TGCTCTCTTA CCGGTCAGTT
    GACATCCGGA AGAGTTTGCA GTTGGAGGAG CTGCTGGCCA GGGAGCAGCT GGAGTACACC
    ATCGAGGAGG AGGTGGCCAA GCAGACCATC CGCATGTGGC TGAAGAAGTG CTTAAAGCGC
    ATTAGAGCTA AACAGCAGCA GTCCTGCAGC ATCATCCACA GCCTGAGGGA GAGCCAACAG
    CAGGAGCTGA GCCGCTTCCT GAATCCTCCC AGTATCGAGA CCACCCAGCC CAGTGAGGAC
    ATGAACGCTA ACAGTCAGGA TAATAATATG CAACCTGAGA CCAGCAGCCA GCAGCAGCTT
    CTCAGCCCCA CGCTGTCGGA CAGGGGGGGC GGCCGGCAGG ACTCCGCGGA CCCCGGGAAG
    CCCCAGAGGA AGATCGGACA GTGGCGGCTG CCCTCAGCAC CAAAACCAAT AAGCCACTCA
    GTGTCTTCGG TCAACTTACG ATTTGCAGGG CGCACAACAA TGAAATCCGT AGTGTGCAAA
    ATGAACCCCA TGACCGACAC GGCGTCGTGT GGTTCAGAGG TTAAGAAGTG GTGGACGCGG
    CAGCTGACAG TGGAAAGCGA CGAAAGTGGC GATGACCTCC TGGATATTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_542654.4
    CDS293..5623
    Translation

    Target ORF information:

    RefSeq Version XM_542654.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris sodium leak channel, non selective (NALCN), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_542654.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAGGAGAC AAGACGACCC CCCAGAGACA CTCAATATCA TGCTTCCTGC TGGCACATCT 
    AGGAACCACA CTGCGACTGC AGTTTTGTAC TTGCTCACCA AAGCTAACTT CAGCATGCTC
    AAAAGGAAGC AGAGCTCCAG GGTGGAGGCC CAGCCAGTTA CTGACTTTGG TCCCGACGAA
    TCTCTGTCCG ACAACGCTGA CATACTCTGG ATTAATAAGC CATGGGTTCA CTCTTTGCTG
    CGCATCTGTG CCATCATCAG TGTCATTTCT GTCTGTATGA ACACACCCAT GACGTTCGAA
    CACTACCCCC CTCTTCAGTA TGTGACTTTT ACTCTGGATA CTTTACTGAT GTTTCTCTAC
    ACAGCAGAGA TGATAGCAAA AATGCACATC CGGGGTATTG TCAAGGGGGA TAGTTCCTAT
    GTGAAAGATC GCTGGTGTGT TTTTGATGGA TTTATGGTCT TCTGCCTTTG GGTTTCATTG
    GTGTTACAGG TATTTGAAAT TGCTGATATA GTTGACCAGA TGTCACCTTG GGGCATGTTG
    CGGATCCCAC GGCCACTAAT TATGATTCGG GCATTCCGGA TTTATTTCCG ATTTGAGCTA
    CCAAGGACCA GAATTACAAA CATTTTAAAG CGATCAGGAG AACAAATATG GAGCGTTTCA
    ATTTTCCTAC TTTTCTTTCT ACTTCTTTAT GGAATTTTAG GAGTTCAGAT GTTTGGAACA
    TTTACCTATC ACTGTGTAGT AAATGACACG AAACCAGGAA ACGTAACCTG GAATAGCTTA
    GCTATCCCAG ATACGCACTG CTCACCAGAG CTGGAGGAGG GCTACCAGTG CCCCCCAGGA
    TTTAAATGCA TGGACCTGGA AGACCTGGGA CTTAGCAGGC AAGAGCTGGG CTACAGTGGC
    TTTAATGAGA TAGGCACCAG CATCTTCACG GTCTATGAGG CCGCGTCCCA GGAAGGCTGG
    GTGTTCCTCA TGTACAGAGC TATTGACAGC TTTCCCCGCT GGCGCTCCTA CTTCTACTTC
    ATCACTCTTA TCTTCTTCCT GGCCTGGCTC GTTAAGAATG TATTTATCGC TGTCATCATT
    GAAACATTTG CAGAAATCAG GGTGCAGTTT CAACAAATGT GGGGATCGAG AAGCAATACT
    ACGTCAACGG CCACGACGCA GATGTTTCAT GAAGACGCTG CCGGCGGATG GCAGCTGGTA
    GCTGTGGATG TCAACAAGCC CCAGGGACGC GCCCCAGCCT GCCTCCAGCA AATGATGCGG
    TCATCAGTTT TCCACATGTT CATTCTGAGC ATGGTGACTG TGGATGTGAT AGTGGCTGCA
    AGCAACTACC ACAAAGGAGA AAGCTTCAGA AGGCAGTACG ATGAGTTCTA TCTTGCAGAG
    GTGGCTTTCA CAGTACTTTT TGATTTGGAA GCACTTCTGA AGATATGGTG TTTAGGATTT
    ACTGGATATA TCAGCTCTTC TCTCCACAAA TTTGAACTAC TACTCGTAAT TGGAACTACT
    CTTCATGTAT ATCCAGATCT TTATCATTCT CAGTTCACAT ACTTTCAGGT TCTTCGGGTA
    GTTCGACTTA TTAAGATCTC ACCTGCACTA GAAGACTTTG TGTACAAGAT ATTTGGTCCT
    GGAAAAAAGC TTGGGAGTTT GGTTGTATTT ACTGCCAGCC TCTTGATTGT TATGTCAGCA
    ATTAGTCTGC AGATGTTCTG CTTTGTCGAA GAACTGGACA GGTTTACAAC GTTTCCAAGG
    GCATTTATGT CCATGTTCCA GATCCTCACC CAGGAAGGAT GGGTAGACGT GATGGACCAA
    ACCTTGAATG CTGTGGGACA CATGTGGGCA CCTGTGGTGG CCATCTATTT CATTTTGTAC
    CATCTCTTTG CCACTCTGAT CCTCCTGAGT TTGTTTGTTG CGGTTATTTT GGACAACTTA
    GAACTTGATG AAGATCTAAA GAAGCTTAAA CAATTAAAAC AAAGTGAAGC GAATGCGGAC
    ACCAAAGAAA AGCTCCCCTT GCGACTTCGA ATCTTTGAAA AATTTCCAAA CAGACCGCAA
    ATGGTGAAAA TCTCAAAGCT TCCTTCAGAT TTTACAGTTC CTAAAATCAG GGAAAGTTTC
    ATGAAGCAGT TTATTGATCG CCAGCAACAG GACACCTGCT GCCTCTTACG AAGCCTTCCG
    TCCACCTCTT CTTCATCGTG TGATCACTCC AAACGGTCTG CAATTGAAGA CAACAAATAC
    ATTGACCAAA AACTTCGCAA GTCTGTTTTC AGCATCAGGG CAAGGAATCT TCTAGAGAAG
    GAGACTGCAG TCACTAAAAT CTTAAGAGCT TGCACTCGAC AGCGCATGCT GAGCGGATCA
    TTTGAGGGGC AGCCAGCAAA GGAAAGGTCA ATTCTCAGTG TGCAGCATCA TATCCGCCAG
    GAGCGCAGGT CACTGAGACA TGGATCCAAC AGCCAGAGGA TCAGCAGGGG CAAATCTCTT
    GAAACCCTGA CTCAAGACCA CTCCAATACA GTGAGATACA GAAATGCACA GAGAGAAGAC
    AGTGAAATCA AGATGATCCA GGAAAAAAAG GAGCAAGCAG AAATGAAAAG GAAAGTGCAG
    GAAGAGGAGC TGAGAGAGAA TCACCCATAC TTCGACAAGC CACTGTTCAT AGTTGGACGG
    GAGCACAGGT TCAGAAACTT CTGCCGGGTG GTGGTCCGGG CACGCTTCAA TGCATCTAAA
    ACAGATCCCG TCACAGGAGC TGTGAAAAAT ACAAAGTACC ATCAACTTTA TGATTTGCTG
    GGCTTGGTGA CTTACCTGGA CTGGGTCATG ATCATCGTAA CCATCTGTTC CTGCATTTCC
    ATGATGTTTG AGTCCCCCTT TCGAAGAGTC ATGCACGCCC CAACTTTGCA GATTGCCGAG
    TACGTGTTTG TGATATTCAT GAGCATCGAG CTTAATCTGA AGATTATGGC AGATGGCTTA
    TTTTTCACTC CAACTGCTGT CATCAGAGAC TTTGGTGGAG TCATGGACAT ATTTATATAT
    CTTGTAAGCT TGATATTTCT TTGTTGGATG CCTCAAAATG TGCCTGCTGA ATCTGGAGCT
    CAGCTTTTAA TGGTTCTGCG GTGCCTGAGA CCTCTGCGAA TATTCAAACT GGTGCCCCAG
    ATGAGGAAAG TTGTTCGAGA ACTTTTCAGT GGCTTCAAGG AAATTTTCTT GGTCTCCATT
    CTTTTGTTGA CATTAATGCT TGTATTTGCA AGCTTTGGAG TTCAGCTTTT TGCGGGGAAA
    CTGGCAAAGT GCAACGATCC CAACATTATT AGAAGGGAAG ATTGTAACGG CATATTCAGA
    ATTAATGTAA GTGTGTCCAA GAACTTAAAT TTAAAATTAA GACCTGGAGA GAAAAAGCCT
    GGATTTTGGG TGCCCCGTGT TTGGGCAAAT CCTAGGAATT TTAATTTTGA CAATGTGGGA
    AATGCTATGC TGGCATTATT TGAAGTGCTG TCCCTGAAAG GCTGGGTGGA AGTGAGGGAT
    GTTATCATTC ATCGTGTGGG GCCGATGCAT GGGATCTATA TTCATGTTTT TGTATTCCTG
    GGTTGCATGA TTGGACTGAC CCTTTTTGTT GGAGTAGTTA TTGCTAATTT CAATGAAAAC
    AAGGGGACAG CTCTGCTGAC TGTAGATCAG AGAAGATGGG AAGATCTGAA GAGCCGATTG
    AAGATAGCCC AGCCTCTTCA TCTCCCACCT CGCCCGGATA ATGATGGTTT TAGAGCTAAG
    ATGTATGACA TCACCCAGCA TCCGTTCTTT AAGAGGACAA TCGCATTGCT TGTTTTGGCC
    CAGTCGGTGC TGCTGTCCGT CAAGTGGGAC GTGGAGGACC CAGTGACTGT CCCTTTGGCA
    ACCATGTCAG TTGTTTTCAC CTTCATCTTT GTTCTAGAGG TTACAATGAA GATCATAGCC
    ATGTCGCCCG CCGGCTTCTG GCAAAGCAGA AGAAACCGAT ACGACCTGCT GGTGACGTCA
    CTTGGTGTGG TCTGGGTGGT ACTTCACTTT GCCCTCCTGA ACGCATATAC CTACATGATG
    GGTGCCTGCG TGATTGTCTT CCGGTTTTTC TCTATCTGTG GAAAGCATGT AACGCTAAAG
    ATGCTCCTCC TGACAGTGGT TGTCAGCATG TATAAAAGCT TCTTCATCAT AGTAGGGATG
    TTCCTTTTGC TCTTGTGTTA TGCTTTTGCT GGAGTTGTTT TATTTGGTAC TGTGAAATAT
    GGAGAGAACA TTAACAGGCA TGCCAACTTT TCTTCAGCTG GCAAAGCCAT CACCGTACTG
    TTCCGAATTG TCACAGGCGA AGACTGGAAT AAGATTATGC ATGACTGTAT GGTCCAGCCT
    CCCTTTTGCA CACCAGATGA ATTCACATAC TGGGCAACAG ACTGTGGAAA TTATGCCGGG
    GCGCTTATGT ATTTCTGTTC ATTTTATGTC ATCATTGCCT ACATCATGCT AAATCTGCTT
    GTAGCCATCA TTGTGGAGAA TTTCTCCTTG TTTTATTCCA CGGAGGAGGA CCAGCTCCTA
    AGTTACAATG ATCTCCGCCA CTTTCAAATC ATATGGAATA TGGTGGATGA TAAACGAGAG
    GGGGTGATTC CAACATTCCG GGTAAAGTTC CTGCTGAGGC TGCTGCGGGG GAGGCTGGAG
    GTGGATCTAG ACAAAGACAA GCTCCTGTTT AAGCACATGT GCTATGAAAT GGAGAGGCTG
    CATAATGGCG GCGATGTCAC TTTCCATGAC GTGCTCAGCA TGCTCTCTTA CCGGTCAGTT
    GACATCCGGA AGAGTTTGCA GTTGGAGGAG CTGCTGGCCA GGGAGCAGCT GGAGTACACC
    ATCGAGGAGG AGGTGGCCAA GCAGACCATC CGCATGTGGC TGAAGAAGTG CTTAAAGCGC
    ATTAGAGCTA AACAGCAGCA GTCCTGCAGC ATCATCCACA GCCTGAGGGA GAGCCAACAG
    CAGGAGCTGA GCCGCTTCCT GAATCCTCCC AGTATCGAGA CCACCCAGCC CAGTGAGGAC
    ATGAACGCTA ACAGTCAGGA TAATAATATG CAACCTGAGA CCAGCAGCCA GCAGCAGCTT
    CTCAGCCCCA CGCTGTCGGA CAGGGGGGGC GGCCGGCAGG ACTCCGCGGA CCCCGGGAAG
    CCCCAGAGGA AGATCGGACA GTGGCGGCTG CCCTCAGCAC CAAAACCAAT AAGCCACTCA
    GTGTCTTCGG TCAACTTACG ATTTGCAGGG CGCACAACAA TGAAATCCGT AGTGTGCAAA
    ATGAACCCCA TGACCGACAC GGCGTCGTGT GGTTCAGAGG TTAAGAAGTG GTGGACGCGG
    CAGCTGACAG TGGAAAGCGA CGAAAGTGGC GATGACCTCC TGGATATTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe23714
    Clone ID Related Accession (Same CDS sequence) XM_005634060.2
    Accession Version XM_005634060.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5217bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product sodium leak channel non-selective protein isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726098.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005634060.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTCAAAA GGAAGCAGAG CTCCAGGGTG GAGGCCCAGC CAGTTACTGA CTTTGGTCCC 
    GACGAATCTC TGTCCGACAA CGCTGACATA CTCTGGATTA ATAAGCCATG GGTTCACTCT
    TTGCTGCGCA TCTGTGCCAT CATCAGTGTC ATTTCTGTCT GTATGAACAC ACCCATGACG
    TTCGAACACT ACCCCCCTCT TCAGTATGTG ACTTTTACTC TGGATACTTT ACTGATGTTT
    CTCTACACAG CAGAGATGAT AGCAAAAATG CACATCCGGG GTATTGTCAA GGGGGATAGT
    TCCTATGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTCTG CCTTTGGGTT
    TCATTGGTGT TACAGGTATT TGAAATTGCT GATATAGTTG ACCAGATGTC ACCTTGGGGC
    ATGTTGCGGA TCCCACGGCC ACTAATTATG ATTCGGGCAT TCCGGATTTA TTTCCGATTT
    GAGCTACCAA GGACCAGAAT TACAAACATT TTAAAGCGAT CAGGAGAACA AATATGGAGC
    GTTTCAATTT TCCTACTTTT CTTTCTACTT CTTTATGGAA TTTTAGGAGT TCAGATGTTT
    GGAACATTTA CCTATCACTG TGTAGTAAAT GACACGAAAC CAGGAAACGT AACCTGGAAT
    AGCTTAGCTA TCCCAGATAC GCACTGCTCA CCAGAGCTGG AGGAGGGCTA CCAGTGCCCC
    CCAGGATTTA AATGCATGGA CCTGGAAGAC CTGGGACTTA GCAGGCAAGA GCTGGGCTAC
    AGTGGCTTTA ATGAGATAGG CACCAGCATC TTCACGGTCT ATGAGGCCGC GTCCCAGGAA
    GGCTGGGTGT TCCTCATGTA CAGAGCTATT GACAGCTTTC CCCGCTGGCG CTCCTACTTC
    TACTTCATCA CTCTTATCTT CTTCCTGGCC TGGCTCGTTA AGAATGTATT TATCGCTGTC
    ATCATTGAAA CATTTGCAGA AATCAGGGTG CAGTTTCAAC AAATGTGGGG ATCGAGAAGC
    AATACTACGT CAACGGCCAC GACGCAGATG TTTCATGAAG ACGCTGCCGG CGGATGGCAG
    CTGGTAGCTG TGGATGTCAA CAAGCCCCAG GGACGCGCCC CAGCCTGCCT CCAGCAAATG
    ATGCGGTCAT CAGTTTTCCA CATGTTCATT CTGAGCATGG TGACTGTGGA TGTGATAGTG
    GCTGCAAGCA ACTACCACAA AGGAGAAAGC TTCAGAAGGC AGTACGATGA GTTCTATCTT
    GCAGAGGTGG CTTTCACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTA
    GGATTTACTG GATATATCAG CTCTTCTCTC CACAAATTTG AACTACTACT CGTAATTGGA
    ACTACTCTTC ATGTATATCC AGATCTTTAT CATTCTCAGT TCACATACTT TCAGGTTCTT
    CGGGTAGTTC GACTTATTAA GATCTCACCT GCACTAGAAG ACTTTGTGTA CAAGATATTT
    GGTCCTGGAA AAAAGCTTGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG
    TCAGCAATTA GTCTGCAGAT GTTCTGCTTT GTCGAAGAAC TGGACAGGTT TACAACGTTT
    CCAAGGGCAT TTATGTCCAT GTTCCAGATC CTCACCCAGG AAGGATGGGT AGACGTGATG
    GACCAAACCT TGAATGCTGT GGGACACATG TGGGCACCTG TGGTGGCCAT CTATTTCATT
    TTGTACCATC TCTTTGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCGGT TATTTTGGAC
    AACTTAGAAC TTGATGAAGA TCTAAAGAAG CTTAAACAAT TAAAACAAAG TGAAGCGAAT
    GCGGACACCA AAGAAAAGCT CCCCTTGCGA CTTCGAATCT TTGAAAAATT TCCAAACAGA
    CCGCAAATGG TGAAAATCTC AAAGCTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAA
    AGTTTCATGA AGCAGTTTAT TGATCGCCAG CAACAGGACA CCTGCTGCCT CTTACGAAGC
    CTTCCGTCCA CCTCTTCTTC ATCGTGTGAT CACTCCAAAC GGTCTGCAAT TGAAGACAAC
    AAATACATTG ACCAAAAACT TCGCAAGTCT GTTTTCAGCA TCAGGGCAAG GAATCTTCTA
    GAGAAGGAGA CTGCAGTCAC TAAAATCTTA AGAGCTTGCA CTCGACAGCG CATGCTGAGC
    GGATCATTTG AGGGGCAGCC AGCAAAGGAA AGGTCAATTC TCAGTGTGCA GCATCATATC
    CGCCAGGAGC GCAGGTCACT GAGACATGGA TCCAACAGCC AGAGGATCAG CAGGGGCAAA
    TCTCTTGAAA CCCTGACTCA AGACCACTCC AATACAGTGA GATACAGAAA TGCACAGAGA
    GAAGACAGTG AAATCAAGAT GATCCAGGAA AAAAAGGAGC AAGCAGAAAT GAAAAGGAAA
    GTGCAGGAAG AGGAGCTGAG AGAGAATCAC CCATACTTCG ACAAGCCACT GTTCATAGTT
    GGACGGGAGC ACAGGTTCAG AAACTTCTGC CGGGTGGTGG TCCGGGCACG CTTCAATGCA
    TCTAAAACAG ATCCCGTCAC AGGAGCTGTG AAAAATACAA AGTACCATCA ACTTTATGAT
    TTGCTGGGCT TGGTGACTTA CCTGGACTGG GTCATGATCA TCGTAACCAT CTGTTCCTGC
    ATTTCCATGA TGTTTGAGTC CCCCTTTCGA AGAGTCATGC ACGCCCCAAC TTTGCAGATT
    GCCGAGTACG TGTTTGTGAT ATTCATGAGC ATCGAGCTTA ATCTGAAGAT TATGGCAGAT
    GGCTTATTTT TCACTCCAAC TGCTGTCATC AGAGACTTTG GTGGAGTCAT GGACATATTT
    ATATATCTTG TAAGCTTGAT ATTTCTTTGT TGGATGCCTC AAAATGTGCC TGCTGAATCT
    GGAGCTCAGC TTTTAATGGT TCTGCGGTGC CTGAGACCTC TGCGAATATT CAAACTGGTG
    CCCCAGATGA GGAAAGTTGT TCGAGAACTT TTCAGTGGCT TCAAGGAAAT TTTCTTGGTC
    TCCATTCTTT TGTTGACATT AATGCTTGTA TTTGCAAGCT TTGGAGTTCA GCTTTTTGCG
    GGGAAACTGG CAAAGTGCAA CGATCCCAAC ATTATTAGAA GGGAAGATTG TAACGGCATA
    TTCAGAATTA ATGTAAGTGT GTCCAAGAAC TTAAATTTAA AATTAAGACC TGGAGAGAAA
    AAGCCTGGAT TTTGGGTGCC CCGTGTTTGG GCAAATCCTA GGAATTTTAA TTTTGACAAT
    GTGGGAAATG CTATGCTGGC ATTATTTGAA GTGCTGTCCC TGAAAGGCTG GGTGGAAGTG
    AGGGATGTTA TCATTCATCG TGTGGGGCCG ATGCATGGGA TCTATATTCA TGTTTTTGTA
    TTCCTGGGTT GCATGATTGG ACTGACCCTT TTTGTTGGAG TAGTTATTGC TAATTTCAAT
    GAAAACAAGG GGACAGCTCT GCTGACTGTA GATCAGAGAA GATGGGAAGA TCTGAAGAGC
    CGATTGAAGA TAGCCCAGCC TCTTCATCTC CCACCTCGCC CGGATAATGA TGGTTTTAGA
    GCTAAGATGT ATGACATCAC CCAGCATCCG TTCTTTAAGA GGACAATCGC ATTGCTTGTT
    TTGGCCCAGT CGGTGCTGCT GTCCGTCAAG TGGGACGTGG AGGACCCAGT GACTGTCCCT
    TTGGCAACCA TGTCAGTTGT TTTCACCTTC ATCTTTGTTC TAGAGGTTAC AATGAAGATC
    ATAGCCATGT CGCCCGCCGG CTTCTGGCAA AGCAGAAGAA ACCGATACGA CCTGCTGGTG
    ACGTCACTTG GTGTGGTCTG GGTGGTACTT CACTTTGCCC TCCTGAACGC ATATACCTAC
    ATGATGGGTG CCTGCGTGAT TGTCTTCCGG TTTTTCTCTA TCTGTGGAAA GCATGTAACG
    CTAAAGATGC TCCTCCTGAC AGTGGTTGTC AGCATGTATA AAAGCTTCTT CATCATAGTA
    GGGATGTTCC TTTTGCTCTT GTGTTATGCT TTTGCTGGAG TTGTTTTATT TGGTACTGTG
    AAATATGGAG AGAACATTAA CAGGCATGCC AACTTTTCTT CAGCTGGCAA AGCCATCACC
    GTACTGTTCC GAATTGTCAC AGGCGAAGAC TGGAATAAGA TTATGCATGA CTGTATGGTC
    CAGCCTCCCT TTTGCACACC AGATGAATTC ACATACTGGG CAACAGACTG TGGAAATTAT
    GCCGGGGCGC TTATGTATTT CTGTTCATTT TATGTCATCA TTGCCTACAT CATGCTAAAT
    CTGCTTGTAG CCATCATTGT GGAGAATTTC TCCTTGTTTT ATTCCACGGA GGAGGACCAG
    CTCCTAAGTT ACAATGATCT CCGCCACTTT CAAATCATAT GGAATATGGT GGATGATAAA
    CGAGAGGGGG TGATTCCAAC ATTCCGGGTA AAGTTCCTGC TGAGGCTGCT GCGGGGGAGG
    CTGGAGGTGG ATCTAGACAA AGACAAGCTC CTGTTTAAGC ACATGTGCTA TGAAATGGAG
    AGGCTGCATA ATGGCGGCGA TGTCACTTTC CATGACGTGC TCAGCATGCT CTCTTACCGG
    TCAGTTGACA TCCGGAAGAG TTTGCAGTTG GAGGAGCTGC TGGCCAGGGA GCAGCTGGAG
    TACACCATCG AGGAGGAGGT GGCCAAGCAG ACCATCCGCA TGTGGCTGAA GAAGTGCTTA
    AAGCGCATTA GAGCTAAACA GCAGCAGTCC TGCAGCATCA TCCACAGCCT GAGGGAGAGC
    CAACAGCAGG AGCTGAGCCG CTTCCTGAAT CCTCCCAGTA TCGAGACCAC CCAGCCCAGT
    GAGGACATGA ACGCTAACAG TCAGGATAAT AATATGCAAC CTGAGACCAG CAGCCAGCAG
    CAGCTTCTCA GCCCCACGCT GTCGGACAGG GGGGGCGGCC GGCAGGACTC CGCGGACCCC
    GGGAAGCCCC AGAGGAAGAT CGGACAGTGG CGGCTGCCCT CAGCACCAAA ACCAATAAGC
    CACTCAGTGT CTTCGGTCAA CTTACGATTT GCAGGGCGCA CAACAATGAA ATCCGTAGTG
    TGCAAAATGA ACCCCATGAC CGACACGGCG TCGTGTGGTT CAGAGGTTAA GAAGTGGTGG
    ACGCGGCAGC TGACAGTGGA AAGCGACGAA AGTGGCGATG ACCTCCTGGA TATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005634117.1
    CDS61..5277
    Translation

    Target ORF information:

    RefSeq Version XM_005634060.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris sodium leak channel, non selective (NALCN), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005634060.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTCAAAA GGAAGCAGAG CTCCAGGGTG GAGGCCCAGC CAGTTACTGA CTTTGGTCCC 
    GACGAATCTC TGTCCGACAA CGCTGACATA CTCTGGATTA ATAAGCCATG GGTTCACTCT
    TTGCTGCGCA TCTGTGCCAT CATCAGTGTC ATTTCTGTCT GTATGAACAC ACCCATGACG
    TTCGAACACT ACCCCCCTCT TCAGTATGTG ACTTTTACTC TGGATACTTT ACTGATGTTT
    CTCTACACAG CAGAGATGAT AGCAAAAATG CACATCCGGG GTATTGTCAA GGGGGATAGT
    TCCTATGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTCTG CCTTTGGGTT
    TCATTGGTGT TACAGGTATT TGAAATTGCT GATATAGTTG ACCAGATGTC ACCTTGGGGC
    ATGTTGCGGA TCCCACGGCC ACTAATTATG ATTCGGGCAT TCCGGATTTA TTTCCGATTT
    GAGCTACCAA GGACCAGAAT TACAAACATT TTAAAGCGAT CAGGAGAACA AATATGGAGC
    GTTTCAATTT TCCTACTTTT CTTTCTACTT CTTTATGGAA TTTTAGGAGT TCAGATGTTT
    GGAACATTTA CCTATCACTG TGTAGTAAAT GACACGAAAC CAGGAAACGT AACCTGGAAT
    AGCTTAGCTA TCCCAGATAC GCACTGCTCA CCAGAGCTGG AGGAGGGCTA CCAGTGCCCC
    CCAGGATTTA AATGCATGGA CCTGGAAGAC CTGGGACTTA GCAGGCAAGA GCTGGGCTAC
    AGTGGCTTTA ATGAGATAGG CACCAGCATC TTCACGGTCT ATGAGGCCGC GTCCCAGGAA
    GGCTGGGTGT TCCTCATGTA CAGAGCTATT GACAGCTTTC CCCGCTGGCG CTCCTACTTC
    TACTTCATCA CTCTTATCTT CTTCCTGGCC TGGCTCGTTA AGAATGTATT TATCGCTGTC
    ATCATTGAAA CATTTGCAGA AATCAGGGTG CAGTTTCAAC AAATGTGGGG ATCGAGAAGC
    AATACTACGT CAACGGCCAC GACGCAGATG TTTCATGAAG ACGCTGCCGG CGGATGGCAG
    CTGGTAGCTG TGGATGTCAA CAAGCCCCAG GGACGCGCCC CAGCCTGCCT CCAGCAAATG
    ATGCGGTCAT CAGTTTTCCA CATGTTCATT CTGAGCATGG TGACTGTGGA TGTGATAGTG
    GCTGCAAGCA ACTACCACAA AGGAGAAAGC TTCAGAAGGC AGTACGATGA GTTCTATCTT
    GCAGAGGTGG CTTTCACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTA
    GGATTTACTG GATATATCAG CTCTTCTCTC CACAAATTTG AACTACTACT CGTAATTGGA
    ACTACTCTTC ATGTATATCC AGATCTTTAT CATTCTCAGT TCACATACTT TCAGGTTCTT
    CGGGTAGTTC GACTTATTAA GATCTCACCT GCACTAGAAG ACTTTGTGTA CAAGATATTT
    GGTCCTGGAA AAAAGCTTGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG
    TCAGCAATTA GTCTGCAGAT GTTCTGCTTT GTCGAAGAAC TGGACAGGTT TACAACGTTT
    CCAAGGGCAT TTATGTCCAT GTTCCAGATC CTCACCCAGG AAGGATGGGT AGACGTGATG
    GACCAAACCT TGAATGCTGT GGGACACATG TGGGCACCTG TGGTGGCCAT CTATTTCATT
    TTGTACCATC TCTTTGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCGGT TATTTTGGAC
    AACTTAGAAC TTGATGAAGA TCTAAAGAAG CTTAAACAAT TAAAACAAAG TGAAGCGAAT
    GCGGACACCA AAGAAAAGCT CCCCTTGCGA CTTCGAATCT TTGAAAAATT TCCAAACAGA
    CCGCAAATGG TGAAAATCTC AAAGCTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAA
    AGTTTCATGA AGCAGTTTAT TGATCGCCAG CAACAGGACA CCTGCTGCCT CTTACGAAGC
    CTTCCGTCCA CCTCTTCTTC ATCGTGTGAT CACTCCAAAC GGTCTGCAAT TGAAGACAAC
    AAATACATTG ACCAAAAACT TCGCAAGTCT GTTTTCAGCA TCAGGGCAAG GAATCTTCTA
    GAGAAGGAGA CTGCAGTCAC TAAAATCTTA AGAGCTTGCA CTCGACAGCG CATGCTGAGC
    GGATCATTTG AGGGGCAGCC AGCAAAGGAA AGGTCAATTC TCAGTGTGCA GCATCATATC
    CGCCAGGAGC GCAGGTCACT GAGACATGGA TCCAACAGCC AGAGGATCAG CAGGGGCAAA
    TCTCTTGAAA CCCTGACTCA AGACCACTCC AATACAGTGA GATACAGAAA TGCACAGAGA
    GAAGACAGTG AAATCAAGAT GATCCAGGAA AAAAAGGAGC AAGCAGAAAT GAAAAGGAAA
    GTGCAGGAAG AGGAGCTGAG AGAGAATCAC CCATACTTCG ACAAGCCACT GTTCATAGTT
    GGACGGGAGC ACAGGTTCAG AAACTTCTGC CGGGTGGTGG TCCGGGCACG CTTCAATGCA
    TCTAAAACAG ATCCCGTCAC AGGAGCTGTG AAAAATACAA AGTACCATCA ACTTTATGAT
    TTGCTGGGCT TGGTGACTTA CCTGGACTGG GTCATGATCA TCGTAACCAT CTGTTCCTGC
    ATTTCCATGA TGTTTGAGTC CCCCTTTCGA AGAGTCATGC ACGCCCCAAC TTTGCAGATT
    GCCGAGTACG TGTTTGTGAT ATTCATGAGC ATCGAGCTTA ATCTGAAGAT TATGGCAGAT
    GGCTTATTTT TCACTCCAAC TGCTGTCATC AGAGACTTTG GTGGAGTCAT GGACATATTT
    ATATATCTTG TAAGCTTGAT ATTTCTTTGT TGGATGCCTC AAAATGTGCC TGCTGAATCT
    GGAGCTCAGC TTTTAATGGT TCTGCGGTGC CTGAGACCTC TGCGAATATT CAAACTGGTG
    CCCCAGATGA GGAAAGTTGT TCGAGAACTT TTCAGTGGCT TCAAGGAAAT TTTCTTGGTC
    TCCATTCTTT TGTTGACATT AATGCTTGTA TTTGCAAGCT TTGGAGTTCA GCTTTTTGCG
    GGGAAACTGG CAAAGTGCAA CGATCCCAAC ATTATTAGAA GGGAAGATTG TAACGGCATA
    TTCAGAATTA ATGTAAGTGT GTCCAAGAAC TTAAATTTAA AATTAAGACC TGGAGAGAAA
    AAGCCTGGAT TTTGGGTGCC CCGTGTTTGG GCAAATCCTA GGAATTTTAA TTTTGACAAT
    GTGGGAAATG CTATGCTGGC ATTATTTGAA GTGCTGTCCC TGAAAGGCTG GGTGGAAGTG
    AGGGATGTTA TCATTCATCG TGTGGGGCCG ATGCATGGGA TCTATATTCA TGTTTTTGTA
    TTCCTGGGTT GCATGATTGG ACTGACCCTT TTTGTTGGAG TAGTTATTGC TAATTTCAAT
    GAAAACAAGG GGACAGCTCT GCTGACTGTA GATCAGAGAA GATGGGAAGA TCTGAAGAGC
    CGATTGAAGA TAGCCCAGCC TCTTCATCTC CCACCTCGCC CGGATAATGA TGGTTTTAGA
    GCTAAGATGT ATGACATCAC CCAGCATCCG TTCTTTAAGA GGACAATCGC ATTGCTTGTT
    TTGGCCCAGT CGGTGCTGCT GTCCGTCAAG TGGGACGTGG AGGACCCAGT GACTGTCCCT
    TTGGCAACCA TGTCAGTTGT TTTCACCTTC ATCTTTGTTC TAGAGGTTAC AATGAAGATC
    ATAGCCATGT CGCCCGCCGG CTTCTGGCAA AGCAGAAGAA ACCGATACGA CCTGCTGGTG
    ACGTCACTTG GTGTGGTCTG GGTGGTACTT CACTTTGCCC TCCTGAACGC ATATACCTAC
    ATGATGGGTG CCTGCGTGAT TGTCTTCCGG TTTTTCTCTA TCTGTGGAAA GCATGTAACG
    CTAAAGATGC TCCTCCTGAC AGTGGTTGTC AGCATGTATA AAAGCTTCTT CATCATAGTA
    GGGATGTTCC TTTTGCTCTT GTGTTATGCT TTTGCTGGAG TTGTTTTATT TGGTACTGTG
    AAATATGGAG AGAACATTAA CAGGCATGCC AACTTTTCTT CAGCTGGCAA AGCCATCACC
    GTACTGTTCC GAATTGTCAC AGGCGAAGAC TGGAATAAGA TTATGCATGA CTGTATGGTC
    CAGCCTCCCT TTTGCACACC AGATGAATTC ACATACTGGG CAACAGACTG TGGAAATTAT
    GCCGGGGCGC TTATGTATTT CTGTTCATTT TATGTCATCA TTGCCTACAT CATGCTAAAT
    CTGCTTGTAG CCATCATTGT GGAGAATTTC TCCTTGTTTT ATTCCACGGA GGAGGACCAG
    CTCCTAAGTT ACAATGATCT CCGCCACTTT CAAATCATAT GGAATATGGT GGATGATAAA
    CGAGAGGGGG TGATTCCAAC ATTCCGGGTA AAGTTCCTGC TGAGGCTGCT GCGGGGGAGG
    CTGGAGGTGG ATCTAGACAA AGACAAGCTC CTGTTTAAGC ACATGTGCTA TGAAATGGAG
    AGGCTGCATA ATGGCGGCGA TGTCACTTTC CATGACGTGC TCAGCATGCT CTCTTACCGG
    TCAGTTGACA TCCGGAAGAG TTTGCAGTTG GAGGAGCTGC TGGCCAGGGA GCAGCTGGAG
    TACACCATCG AGGAGGAGGT GGCCAAGCAG ACCATCCGCA TGTGGCTGAA GAAGTGCTTA
    AAGCGCATTA GAGCTAAACA GCAGCAGTCC TGCAGCATCA TCCACAGCCT GAGGGAGAGC
    CAACAGCAGG AGCTGAGCCG CTTCCTGAAT CCTCCCAGTA TCGAGACCAC CCAGCCCAGT
    GAGGACATGA ACGCTAACAG TCAGGATAAT AATATGCAAC CTGAGACCAG CAGCCAGCAG
    CAGCTTCTCA GCCCCACGCT GTCGGACAGG GGGGGCGGCC GGCAGGACTC CGCGGACCCC
    GGGAAGCCCC AGAGGAAGAT CGGACAGTGG CGGCTGCCCT CAGCACCAAA ACCAATAAGC
    CACTCAGTGT CTTCGGTCAA CTTACGATTT GCAGGGCGCA CAACAATGAA ATCCGTAGTG
    TGCAAAATGA ACCCCATGAC CGACACGGCG TCGTGTGGTT CAGAGGTTAA GAAGTGGTGG
    ACGCGGCAGC TGACAGTGGA AAGCGACGAA AGTGGCGATG ACCTCCTGGA TATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.