LRRC8E cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LRRC8E
Entrez Gene ID	485003
Full Name	leucine rich repeat containing 8 VRAC subunit E
General protein information	Preferred Names leucine rich repeat containing 8 VRAC subunit E Names volume-regulated anion channel subunit LRRC8E leucine rich repeat containing 8 family member E
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005632945.2	XP_005633002.1	volume-regulated anion channel subunit LRRC8E
XM_005632945.3	XP_005633002.1	volume-regulated anion channel subunit LRRC8E
XM_005632944.2	XP_005633001.1	volume-regulated anion channel subunit LRRC8E
XM_005632944.1	XP_005633001.1	volume-regulated anion channel subunit LRRC8E
XM_542121.5	XP_542121.2	volume-regulated anion channel subunit LRRC8E
XM_542121.6	XP_542121.2	volume-regulated anion channel subunit LRRC8E

Pathways from BioSystems

Homologies

Homo sapiens (human)	LRRC8E	NP_001255213.1
Pan troglodytes (chimpanzee)	LRRC8E	XP_003953389.1
Canis lupus familiaris (dog)	LRRC8E	XP_005633001.1
Bos taurus (cattle)	LRRC8E	NP_001192551.1
Rattus norvegicus (Norway rat)	Lrrc8e	NP_001029311.1
Xenopus tropicalis (tropical clawed frog)	lrrc8e	NP_001007863.1
	LRRC8E	XP_001093244.1
Mus musculus (house mouse)	Lrrc8e	NP_082451.2

Gene Ontology

The following LRRC8E gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LRRC8E cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe22259	XM_005632944.2 Latest version!	Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe22259	XM_005632945.3 Latest version!	Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe22259	XM_542121.6 Latest version!	Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
OCe22259	XM_005632945.2	Canis lupus familiaris leucine rich repeat containing 8 family, member E (LRRC8E), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe22259	XM_005632944.1	Canis lupus familiaris leucine rich repeat containing 8 family, member E (LRRC8E), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe22259	XM_542121.5	Canis lupus familiaris leucine rich repeat containing 8 family, member E (LRRC8E), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LRRC8E ( XM_005632945.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005633002.1 Canis lupus familiaris leucine rich repeat containing 8 family, member E (LRRC8E), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe22259
Clone ID Related Accession (Same CDS sequence)	XM_005632945.2 , XM_005632945.3 , XM_005632944.2 , XM_005632944.1 , XM_542121.5 , XM_542121.6
Accession Version	XM_005632945.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	volume-regulated anion channel subunit LRRC8E
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726096.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005632945.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCCGG TGGCCGAGTT CAAGCAGTTC ACGGAGCAGC AGCCCGCCTT CAAGGTGCTC 
AAACCCTGGT GGGACGTGCT GGCGGAGTAC CTCACCGTGG CTATGCTCAT GATCGGGGTC 
TTCGGCTGCA CCCTGCAGGT GACACAGGAC AAGATCATCT GTCTGCCCAG TCACGAACCC 
CGGGAGAATT TATCAGAGGC CCCATGCCAG CAACTGCTGC CACGGGGGGT CTCCGAGCAG 
ATGGCGGGCC TTCGGGAGGT AAGCGGCCTC AAGAACAACC TGGACCTGCA GCAGTACAGC 
TTCATTAACC AGCTCTGCTA CGAGACAGCC CTCCACTGGT ACGCCAAGTA CTTCCCCTAC 
CTGGTCGTCA TTCACACGCT CATCTTCATG GTCTGCACCA GCTTCTGGTT CAAGTTCCCT 
GGCACCAGCT CCAAGATCGA GCACTTCATC TCCATCCTGG GCAAATGTTT CGACTCGCCA 
TGGACCACAC GGGCCCTGTC TGAGGTCTCT GGGGAGAACC ATAAGGGCCC CGCCGCTGGA 
CGGGTGACGG CCACCGTGGA GGCCTCCGCG GGACCAGGAA AAGCTAATGA GGGTGAGAAG 
GAAAAGGTGC TGGTGGAGCC TGAGAAGGTG GTGACGGAGC CACCAGCCGT CACCCTGTTG 
GATAAGAAGG AGGGTGAACA GGCCAAAGCC CTGTTTGAGA AGGTCAAGAA GTTCCGTGTG 
CATGTGGAGG AGGGGGACAT CTTGTATACC ATGTACATCC GGCAAACGGT GCTCAAGGTC 
TGCAAGTTCT TTGCCATCCT GGTCTACAAC CTTGTCTACG TGGAGAAGAT CAGTTTCCTG 
GTGGCCTGCA GGGTAGAGAC GTCGGAGGTC ACGGGCTACG CCAGCTTCTG CTGCAACCAC 
ACCAAGGCCC ACCTCTTCTC CAAGCTGGCT TTCTGCTACA TCTCCTTCGT GTGCGTCTAT 
GGGCTAACCT GTCTCTACAC GCTCTACTGG CTCTTCCATC GGCCCCTCAA GGAGTACTCC 
TTCCGTTCTG TGCGGGAGGA GACCGGCATG GGTGACATAC CCGACGTCAA GAATGACTTT 
GCTTTCATGC TACATCTCAT CGACCAGTAT GACTCCCTCT ACTCCAAGCG CTTCGCCGTC 
TTCCTCTCCG AAGTCAGCGA GAGCCGCCTG AAGCAGCTCA ACCTGAACCA CGAGTGGACG 
CCTGAGAAGC TGCGGCAGAA GCTGCAGCGC AACGCCCGGG GCCGGCTCGA GCTGGCCCTC 
TGCATGCTCC CGGGGCTGCC GGACACCGTC TTCGAGCTCA GCGAGGTGGA AGCCCTCCGG 
CTGGAGGCCA TCTGTGATAT CACCTTCCCC CCCGGCCTCT CGCAGCTGGT GCACCTGCAG 
GAGCTCAGCC TGCTCCACTC GCCTGCCAGG CTGCCCTTCT CGCTGCAGAT CTTCCTGCGG 
GACCGCCTGA AGGTCATCTG CGTCAAGTGC GAGGAGCTCC GCGACGTGCC CCTGTGGGTG 
TTTGGGCTGC GTGGCCTGGA GGAGCTGCAC CTCGAGGGGC TCTTCCCCCC GGAGCTGGCT 
CGGGCAGCGG CCCTAGAGAG CCTCCGGGAG CTGAAGCAGC TCAAGGCGCT GTCTCTGCGG 
AGCAATGCCA GCAAGGTGCC GGCCAGCGTG ACTGACGTGG CCGGGCACCT GCAGCGGCTT 
AGTCTGCACA ACGACGGGGC CCGCCTGCTG GCCCTGAACA GCCTTAAGAA GCTGGCGGCA 
CTGCGGGAGC TGGAGCTGGT GGCCTGCGGG CTGGAGCGCA TCCCCCATGC CATCTTCAGC 
CTGGGCGCGC TGCAGGAACT TGACCTCAGG GACAACCACC TGCGGTCCAT TGAAGAGATC 
CTCAGCTTCC AGCACTGTCG CAAGCTGGTC ACGCTCAAGC TGTGGCACAA CCAGATCGCT 
TACGTCCCTG AGCACGTGCG CAAGCTCCGG GGCCTGGAGC AGCTCTACCT CAGCCACAAC 
AAGCTGGAGA CCCTGCCCAC CCAGCTCGGC ATGTGCTCCG GCCTCCGCCT GCTGGACATC 
TCCCACAATG GGCTACGCTC CCTGCCGCCC GAGCTGGGCC TCCTCCAGAA CCTGCAGCAC 
CTGGCTCTGT CCTACAATGC CCTGGAGGCC CTGCCTGATG AGCTCTTCTT CTGCCGGAAG 
CTGCGGACGT TGCTTCTGGG CTACAACCAC CTGAGCCAGC TCTCGTCCCA GGTGGGGGCC 
CTCAGAGCCC TCAGCCGCCT GGAGCTCAAG GGCAACCGCT TGGAGGCACT ACCAGAAGAG 
CTTGGCAACT GTGTGGGGCT CAAGAAAGTG GGGATCCTCG TAGAAGACAC CCTTTACGAG 
GGCCTGCCGG CAGAGGTGCG GGAGAAACTG GAGGAGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005633002.1
CDS	99..2480
Translation	MIPVAEFKQFTEQQPAFKVLKPWWDVLAEYLTVAMLMIGVFGCTLQVTQDKIICLPSHEPRENLSEAPCQQLLPRGVSEQMAGLREVSGLKNNLDLQQYSFINQLCYETALHWYAKYFPYLVVIHTLIFMVCTSFWFKFPGTSSKIEHFISILGKCFDSPWTTRALSEVSGENHKGPAAGRVTATVEASAGPGKANEGEKEKVLVEPEKVVTEPPAVTLLDKKEGEQAKALFEKVKKFRVHVEEGDILYTMYIRQTVLKVCKFFAILVYNLVYVEKISFLVACRVETSEVTGYASFCCNHTKAHLFSKLAFCYISFVCVYGLTCLYTLYWLFHRPLKEYSFRSVREETGMGDIPDVKNDFAFMLHLIDQYDSLYSKRFAVFLSEVSESRLKQLNLNHEWTPEKLRQKLQRNARGRLELALCMLPGLPDTVFELSEVEALRLEAICDITFPPGLSQLVHLQELSLLHSPARLPFSLQIFLRDRLKVICVKCEELRDVPLWVFGLRGLEELHLEGLFPPELARAAALESLRELKQLKALSLRSNASKVPASVTDVAGHLQRLSLHNDGARLLALNSLKKLAALRELELVACGLERIPHAIFSLGALQELDLRDNHLRSIEEILSFQHCRKLVTLKLWHNQIAYVPEHVRKLRGLEQLYLSHNKLETLPTQLGMCSGLRLLDISHNGLRSLPPELGLLQNLQHLALSYNALEALPDELFFCRKLRTLLLGYNHLSQLSSQVGALRALSRLELKGNRLEALPEELGNCVGLKKVGILVEDTLYEGLPAEVREKLEEE

Target ORF information:

RefSeq Version	XM_005632945.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris leucine rich repeat containing 8 family, member E (LRRC8E), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005632945.2

ORF Insert Sequence:

ATGATTCCGG TGGCCGAGTT CAAGCAGTTC ACGGAGCAGC AGCCCGCCTT CAAGGTGCTC 
AAACCCTGGT GGGACGTGCT GGCGGAGTAC CTCACCGTGG CTATGCTCAT GATCGGGGTC 
TTCGGCTGCA CCCTGCAGGT GACACAGGAC AAGATCATCT GTCTGCCCAG TCACGAACCC 
CGGGAGAATT TATCAGAGGC CCCATGCCAG CAACTGCTGC CACGGGGGGT CTCCGAGCAG 
ATGGCGGGCC TTCGGGAGGT AAGCGGCCTC AAGAACAACC TGGACCTGCA GCAGTACAGC 
TTCATTAACC AGCTCTGCTA CGAGACAGCC CTCCACTGGT ACGCCAAGTA CTTCCCCTAC 
CTGGTCGTCA TTCACACGCT CATCTTCATG GTCTGCACCA GCTTCTGGTT CAAGTTCCCT 
GGCACCAGCT CCAAGATCGA GCACTTCATC TCCATCCTGG GCAAATGTTT CGACTCGCCA 
TGGACCACAC GGGCCCTGTC TGAGGTCTCT GGGGAGAACC ATAAGGGCCC CGCCGCTGGA 
CGGGTGACGG CCACCGTGGA GGCCTCCGCG GGACCAGGAA AAGCTAATGA GGGTGAGAAG 
GAAAAGGTGC TGGTGGAGCC TGAGAAGGTG GTGACGGAGC CACCAGCCGT CACCCTGTTG 
GATAAGAAGG AGGGTGAACA GGCCAAAGCC CTGTTTGAGA AGGTCAAGAA GTTCCGTGTG 
CATGTGGAGG AGGGGGACAT CTTGTATACC ATGTACATCC GGCAAACGGT GCTCAAGGTC 
TGCAAGTTCT TTGCCATCCT GGTCTACAAC CTTGTCTACG TGGAGAAGAT CAGTTTCCTG 
GTGGCCTGCA GGGTAGAGAC GTCGGAGGTC ACGGGCTACG CCAGCTTCTG CTGCAACCAC 
ACCAAGGCCC ACCTCTTCTC CAAGCTGGCT TTCTGCTACA TCTCCTTCGT GTGCGTCTAT 
GGGCTAACCT GTCTCTACAC GCTCTACTGG CTCTTCCATC GGCCCCTCAA GGAGTACTCC 
TTCCGTTCTG TGCGGGAGGA GACCGGCATG GGTGACATAC CCGACGTCAA GAATGACTTT 
GCTTTCATGC TACATCTCAT CGACCAGTAT GACTCCCTCT ACTCCAAGCG CTTCGCCGTC 
TTCCTCTCCG AAGTCAGCGA GAGCCGCCTG AAGCAGCTCA ACCTGAACCA CGAGTGGACG 
CCTGAGAAGC TGCGGCAGAA GCTGCAGCGC AACGCCCGGG GCCGGCTCGA GCTGGCCCTC 
TGCATGCTCC CGGGGCTGCC GGACACCGTC TTCGAGCTCA GCGAGGTGGA AGCCCTCCGG 
CTGGAGGCCA TCTGTGATAT CACCTTCCCC CCCGGCCTCT CGCAGCTGGT GCACCTGCAG 
GAGCTCAGCC TGCTCCACTC GCCTGCCAGG CTGCCCTTCT CGCTGCAGAT CTTCCTGCGG 
GACCGCCTGA AGGTCATCTG CGTCAAGTGC GAGGAGCTCC GCGACGTGCC CCTGTGGGTG 
TTTGGGCTGC GTGGCCTGGA GGAGCTGCAC CTCGAGGGGC TCTTCCCCCC GGAGCTGGCT 
CGGGCAGCGG CCCTAGAGAG CCTCCGGGAG CTGAAGCAGC TCAAGGCGCT GTCTCTGCGG 
AGCAATGCCA GCAAGGTGCC GGCCAGCGTG ACTGACGTGG CCGGGCACCT GCAGCGGCTT 
AGTCTGCACA ACGACGGGGC CCGCCTGCTG GCCCTGAACA GCCTTAAGAA GCTGGCGGCA 
CTGCGGGAGC TGGAGCTGGT GGCCTGCGGG CTGGAGCGCA TCCCCCATGC CATCTTCAGC 
CTGGGCGCGC TGCAGGAACT TGACCTCAGG GACAACCACC TGCGGTCCAT TGAAGAGATC 
CTCAGCTTCC AGCACTGTCG CAAGCTGGTC ACGCTCAAGC TGTGGCACAA CCAGATCGCT 
TACGTCCCTG AGCACGTGCG CAAGCTCCGG GGCCTGGAGC AGCTCTACCT CAGCCACAAC 
AAGCTGGAGA CCCTGCCCAC CCAGCTCGGC ATGTGCTCCG GCCTCCGCCT GCTGGACATC 
TCCCACAATG GGCTACGCTC CCTGCCGCCC GAGCTGGGCC TCCTCCAGAA CCTGCAGCAC 
CTGGCTCTGT CCTACAATGC CCTGGAGGCC CTGCCTGATG AGCTCTTCTT CTGCCGGAAG 
CTGCGGACGT TGCTTCTGGG CTACAACCAC CTGAGCCAGC TCTCGTCCCA GGTGGGGGCC 
CTCAGAGCCC TCAGCCGCCT GGAGCTCAAG GGCAACCGCT TGGAGGCACT ACCAGAAGAG 
CTTGGCAACT GTGTGGGGCT CAAGAAAGTG GGGATCCTCG TAGAAGACAC CCTTTACGAG 
GGCCTGCCGG CAGAGGTGCG GGAGAAACTG GAGGAGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRC8E ( XM_005632945.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005633002.1 Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe22259
Clone ID Related Accession (Same CDS sequence)	XM_005632945.2 , XM_005632945.3 , XM_005632944.2 , XM_005632944.1 , XM_542121.5 , XM_542121.6
Accession Version	XM_005632945.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	volume-regulated anion channel subunit LRRC8E
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005632945.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCCGG TGGCCGAGTT CAAGCAGTTC ACGGAGCAGC AGCCCGCCTT CAAGGTGCTC 
AAACCCTGGT GGGACGTGCT GGCGGAGTAC CTCACCGTGG CTATGCTCAT GATCGGGGTC 
TTCGGCTGCA CCCTGCAGGT GACACAGGAC AAGATCATCT GTCTGCCCAG TCACGAACCC 
CGGGAGAATT TATCAGAGGC CCCATGCCAG CAACTGCTGC CACGGGGGGT CTCCGAGCAG 
ATGGCGGGCC TTCGGGAGGT AAGCGGCCTC AAGAACAACC TGGACCTGCA GCAGTACAGC 
TTCATTAACC AGCTCTGCTA CGAGACAGCC CTCCACTGGT ACGCCAAGTA CTTCCCCTAC 
CTGGTCGTCA TTCACACGCT CATCTTCATG GTCTGCACCA GCTTCTGGTT CAAGTTCCCT 
GGCACCAGCT CCAAGATCGA GCACTTCATC TCCATCCTGG GCAAATGTTT CGACTCGCCA 
TGGACCACAC GGGCCCTGTC TGAGGTCTCT GGGGAGAACC ATAAGGGCCC CGCCGCTGGA 
CGGGTGACGG CCACCGTGGA GGCCTCCGCG GGACCAGGAA AAGCTAATGA GGGTGAGAAG 
GAAAAGGTGC TGGTGGAGCC TGAGAAGGTG GTGACGGAGC CACCAGCCGT CACCCTGTTG 
GATAAGAAGG AGGGTGAACA GGCCAAAGCC CTGTTTGAGA AGGTCAAGAA GTTCCGTGTG 
CATGTGGAGG AGGGGGACAT CTTGTATACC ATGTACATCC GGCAAACGGT GCTCAAGGTC 
TGCAAGTTCT TTGCCATCCT GGTCTACAAC CTTGTCTACG TGGAGAAGAT CAGTTTCCTG 
GTGGCCTGCA GGGTAGAGAC GTCGGAGGTC ACGGGCTACG CCAGCTTCTG CTGCAACCAC 
ACCAAGGCCC ACCTCTTCTC CAAGCTGGCT TTCTGCTACA TCTCCTTCGT GTGCGTCTAT 
GGGCTAACCT GTCTCTACAC GCTCTACTGG CTCTTCCATC GGCCCCTCAA GGAGTACTCC 
TTCCGTTCTG TGCGGGAGGA GACCGGCATG GGTGACATAC CCGACGTCAA GAATGACTTT 
GCTTTCATGC TACATCTCAT CGACCAGTAT GACTCCCTCT ACTCCAAGCG CTTCGCCGTC 
TTCCTCTCCG AAGTCAGCGA GAGCCGCCTG AAGCAGCTCA ACCTGAACCA CGAGTGGACG 
CCTGAGAAGC TGCGGCAGAA GCTGCAGCGC AACGCCCGGG GCCGGCTCGA GCTGGCCCTC 
TGCATGCTCC CGGGGCTGCC GGACACCGTC TTCGAGCTCA GCGAGGTGGA AGCCCTCCGG 
CTGGAGGCCA TCTGTGATAT CACCTTCCCC CCCGGCCTCT CGCAGCTGGT GCACCTGCAG 
GAGCTCAGCC TGCTCCACTC GCCTGCCAGG CTGCCCTTCT CGCTGCAGAT CTTCCTGCGG 
GACCGCCTGA AGGTCATCTG CGTCAAGTGC GAGGAGCTCC GCGACGTGCC CCTGTGGGTG 
TTTGGGCTGC GTGGCCTGGA GGAGCTGCAC CTCGAGGGGC TCTTCCCCCC GGAGCTGGCT 
CGGGCAGCGG CCCTAGAGAG CCTCCGGGAG CTGAAGCAGC TCAAGGCGCT GTCTCTGCGG 
AGCAATGCCA GCAAGGTGCC GGCCAGCGTG ACTGACGTGG CCGGGCACCT GCAGCGGCTT 
AGTCTGCACA ACGACGGGGC CCGCCTGCTG GCCCTGAACA GCCTTAAGAA GCTGGCGGCA 
CTGCGGGAGC TGGAGCTGGT GGCCTGCGGG CTGGAGCGCA TCCCCCATGC CATCTTCAGC 
CTGGGCGCGC TGCAGGAACT TGACCTCAGG GACAACCACC TGCGGTCCAT TGAAGAGATC 
CTCAGCTTCC AGCACTGTCG CAAGCTGGTC ACGCTCAAGC TGTGGCACAA CCAGATCGCT 
TACGTCCCTG AGCACGTGCG CAAGCTCCGG GGCCTGGAGC AGCTCTACCT CAGCCACAAC 
AAGCTGGAGA CCCTGCCCAC CCAGCTCGGC ATGTGCTCCG GCCTCCGCCT GCTGGACATC 
TCCCACAATG GGCTACGCTC CCTGCCGCCC GAGCTGGGCC TCCTCCAGAA CCTGCAGCAC 
CTGGCTCTGT CCTACAATGC CCTGGAGGCC CTGCCTGATG AGCTCTTCTT CTGCCGGAAG 
CTGCGGACGT TGCTTCTGGG CTACAACCAC CTGAGCCAGC TCTCGTCCCA GGTGGGGGCC 
CTCAGAGCCC TCAGCCGCCT GGAGCTCAAG GGCAACCGCT TGGAGGCACT ACCAGAAGAG 
CTTGGCAACT GTGTGGGGCT CAAGAAAGTG GGGATCCTCG TAGAAGACAC CCTTTACGAG 
GGCCTGCCGG CAGAGGTGCG GGAGAAACTG GAGGAGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005633002.1
CDS	396..2777
Translation	MIPVAEFKQFTEQQPAFKVLKPWWDVLAEYLTVAMLMIGVFGCTLQVTQDKIICLPSHEPRENLSEAPCQQLLPRGVSEQMAGLREVSGLKNNLDLQQYSFINQLCYETALHWYAKYFPYLVVIHTLIFMVCTSFWFKFPGTSSKIEHFISILGKCFDSPWTTRALSEVSGENHKGPAAGRVTATVEASAGPGKANEGEKEKVLVEPEKVVTEPPAVTLLDKKEGEQAKALFEKVKKFRVHVEEGDILYTMYIRQTVLKVCKFFAILVYNLVYVEKISFLVACRVETSEVTGYASFCCNHTKAHLFSKLAFCYISFVCVYGLTCLYTLYWLFHRPLKEYSFRSVREETGMGDIPDVKNDFAFMLHLIDQYDSLYSKRFAVFLSEVSESRLKQLNLNHEWTPEKLRQKLQRNARGRLELALCMLPGLPDTVFELSEVEALRLEAICDITFPPGLSQLVHLQELSLLHSPARLPFSLQIFLRDRLKVICVKCEELRDVPLWVFGLRGLEELHLEGLFPPELARAAALESLRELKQLKALSLRSNASKVPASVTDVAGHLQRLSLHNDGARLLALNSLKKLAALRELELVACGLERIPHAIFSLGALQELDLRDNHLRSIEEILSFQHCRKLVTLKLWHNQIAYVPEHVRKLRGLEQLYLSHNKLETLPTQLGMCSGLRLLDISHNGLRSLPPELGLLQNLQHLALSYNALEALPDELFFCRKLRTLLLGYNHLSQLSSQVGALRALSRLELKGNRLEALPEELGNCVGLKKVGILVEDTLYEGLPAEVREKLEEE

Target ORF information:

RefSeq Version	XM_005632945.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005632945.3

ORF Insert Sequence:

ATGATTCCGG TGGCCGAGTT CAAGCAGTTC ACGGAGCAGC AGCCCGCCTT CAAGGTGCTC 
AAACCCTGGT GGGACGTGCT GGCGGAGTAC CTCACCGTGG CTATGCTCAT GATCGGGGTC 
TTCGGCTGCA CCCTGCAGGT GACACAGGAC AAGATCATCT GTCTGCCCAG TCACGAACCC 
CGGGAGAATT TATCAGAGGC CCCATGCCAG CAACTGCTGC CACGGGGGGT CTCCGAGCAG 
ATGGCGGGCC TTCGGGAGGT AAGCGGCCTC AAGAACAACC TGGACCTGCA GCAGTACAGC 
TTCATTAACC AGCTCTGCTA CGAGACAGCC CTCCACTGGT ACGCCAAGTA CTTCCCCTAC 
CTGGTCGTCA TTCACACGCT CATCTTCATG GTCTGCACCA GCTTCTGGTT CAAGTTCCCT 
GGCACCAGCT CCAAGATCGA GCACTTCATC TCCATCCTGG GCAAATGTTT CGACTCGCCA 
TGGACCACAC GGGCCCTGTC TGAGGTCTCT GGGGAGAACC ATAAGGGCCC CGCCGCTGGA 
CGGGTGACGG CCACCGTGGA GGCCTCCGCG GGACCAGGAA AAGCTAATGA GGGTGAGAAG 
GAAAAGGTGC TGGTGGAGCC TGAGAAGGTG GTGACGGAGC CACCAGCCGT CACCCTGTTG 
GATAAGAAGG AGGGTGAACA GGCCAAAGCC CTGTTTGAGA AGGTCAAGAA GTTCCGTGTG 
CATGTGGAGG AGGGGGACAT CTTGTATACC ATGTACATCC GGCAAACGGT GCTCAAGGTC 
TGCAAGTTCT TTGCCATCCT GGTCTACAAC CTTGTCTACG TGGAGAAGAT CAGTTTCCTG 
GTGGCCTGCA GGGTAGAGAC GTCGGAGGTC ACGGGCTACG CCAGCTTCTG CTGCAACCAC 
ACCAAGGCCC ACCTCTTCTC CAAGCTGGCT TTCTGCTACA TCTCCTTCGT GTGCGTCTAT 
GGGCTAACCT GTCTCTACAC GCTCTACTGG CTCTTCCATC GGCCCCTCAA GGAGTACTCC 
TTCCGTTCTG TGCGGGAGGA GACCGGCATG GGTGACATAC CCGACGTCAA GAATGACTTT 
GCTTTCATGC TACATCTCAT CGACCAGTAT GACTCCCTCT ACTCCAAGCG CTTCGCCGTC 
TTCCTCTCCG AAGTCAGCGA GAGCCGCCTG AAGCAGCTCA ACCTGAACCA CGAGTGGACG 
CCTGAGAAGC TGCGGCAGAA GCTGCAGCGC AACGCCCGGG GCCGGCTCGA GCTGGCCCTC 
TGCATGCTCC CGGGGCTGCC GGACACCGTC TTCGAGCTCA GCGAGGTGGA AGCCCTCCGG 
CTGGAGGCCA TCTGTGATAT CACCTTCCCC CCCGGCCTCT CGCAGCTGGT GCACCTGCAG 
GAGCTCAGCC TGCTCCACTC GCCTGCCAGG CTGCCCTTCT CGCTGCAGAT CTTCCTGCGG 
GACCGCCTGA AGGTCATCTG CGTCAAGTGC GAGGAGCTCC GCGACGTGCC CCTGTGGGTG 
TTTGGGCTGC GTGGCCTGGA GGAGCTGCAC CTCGAGGGGC TCTTCCCCCC GGAGCTGGCT 
CGGGCAGCGG CCCTAGAGAG CCTCCGGGAG CTGAAGCAGC TCAAGGCGCT GTCTCTGCGG 
AGCAATGCCA GCAAGGTGCC GGCCAGCGTG ACTGACGTGG CCGGGCACCT GCAGCGGCTT 
AGTCTGCACA ACGACGGGGC CCGCCTGCTG GCCCTGAACA GCCTTAAGAA GCTGGCGGCA 
CTGCGGGAGC TGGAGCTGGT GGCCTGCGGG CTGGAGCGCA TCCCCCATGC CATCTTCAGC 
CTGGGCGCGC TGCAGGAACT TGACCTCAGG GACAACCACC TGCGGTCCAT TGAAGAGATC 
CTCAGCTTCC AGCACTGTCG CAAGCTGGTC ACGCTCAAGC TGTGGCACAA CCAGATCGCT 
TACGTCCCTG AGCACGTGCG CAAGCTCCGG GGCCTGGAGC AGCTCTACCT CAGCCACAAC 
AAGCTGGAGA CCCTGCCCAC CCAGCTCGGC ATGTGCTCCG GCCTCCGCCT GCTGGACATC 
TCCCACAATG GGCTACGCTC CCTGCCGCCC GAGCTGGGCC TCCTCCAGAA CCTGCAGCAC 
CTGGCTCTGT CCTACAATGC CCTGGAGGCC CTGCCTGATG AGCTCTTCTT CTGCCGGAAG 
CTGCGGACGT TGCTTCTGGG CTACAACCAC CTGAGCCAGC TCTCGTCCCA GGTGGGGGCC 
CTCAGAGCCC TCAGCCGCCT GGAGCTCAAG GGCAACCGCT TGGAGGCACT ACCAGAAGAG 
CTTGGCAACT GTGTGGGGCT CAAGAAAGTG GGGATCCTCG TAGAAGACAC CCTTTACGAG 
GGCCTGCCGG CAGAGGTGCG GGAGAAACTG GAGGAGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRC8E ( XM_005632944.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005633001.1 Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe22259
Clone ID Related Accession (Same CDS sequence)	XM_005632945.2 , XM_005632945.3 , XM_005632944.2 , XM_005632944.1 , XM_542121.5 , XM_542121.6
Accession Version	XM_005632944.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	volume-regulated anion channel subunit LRRC8E
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005632944.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCCGG TGGCCGAGTT CAAGCAGTTC ACGGAGCAGC AGCCCGCCTT CAAGGTGCTC 
AAACCCTGGT GGGACGTGCT GGCGGAGTAC CTCACCGTGG CTATGCTCAT GATCGGGGTC 
TTCGGCTGCA CCCTGCAGGT GACACAGGAC AAGATCATCT GTCTGCCCAG TCACGAACCC 
CGGGAGAATT TATCAGAGGC CCCATGCCAG CAACTGCTGC CACGGGGGGT CTCCGAGCAG 
ATGGCGGGCC TTCGGGAGGT AAGCGGCCTC AAGAACAACC TGGACCTGCA GCAGTACAGC 
TTCATTAACC AGCTCTGCTA CGAGACAGCC CTCCACTGGT ACGCCAAGTA CTTCCCCTAC 
CTGGTCGTCA TTCACACGCT CATCTTCATG GTCTGCACCA GCTTCTGGTT CAAGTTCCCT 
GGCACCAGCT CCAAGATCGA GCACTTCATC TCCATCCTGG GCAAATGTTT CGACTCGCCA 
TGGACCACAC GGGCCCTGTC TGAGGTCTCT GGGGAGAACC ATAAGGGCCC CGCCGCTGGA 
CGGGTGACGG CCACCGTGGA GGCCTCCGCG GGACCAGGAA AAGCTAATGA GGGTGAGAAG 
GAAAAGGTGC TGGTGGAGCC TGAGAAGGTG GTGACGGAGC CACCAGCCGT CACCCTGTTG 
GATAAGAAGG AGGGTGAACA GGCCAAAGCC CTGTTTGAGA AGGTCAAGAA GTTCCGTGTG 
CATGTGGAGG AGGGGGACAT CTTGTATACC ATGTACATCC GGCAAACGGT GCTCAAGGTC 
TGCAAGTTCT TTGCCATCCT GGTCTACAAC CTTGTCTACG TGGAGAAGAT CAGTTTCCTG 
GTGGCCTGCA GGGTAGAGAC GTCGGAGGTC ACGGGCTACG CCAGCTTCTG CTGCAACCAC 
ACCAAGGCCC ACCTCTTCTC CAAGCTGGCT TTCTGCTACA TCTCCTTCGT GTGCGTCTAT 
GGGCTAACCT GTCTCTACAC GCTCTACTGG CTCTTCCATC GGCCCCTCAA GGAGTACTCC 
TTCCGTTCTG TGCGGGAGGA GACCGGCATG GGTGACATAC CCGACGTCAA GAATGACTTT 
GCTTTCATGC TACATCTCAT CGACCAGTAT GACTCCCTCT ACTCCAAGCG CTTCGCCGTC 
TTCCTCTCCG AAGTCAGCGA GAGCCGCCTG AAGCAGCTCA ACCTGAACCA CGAGTGGACG 
CCTGAGAAGC TGCGGCAGAA GCTGCAGCGC AACGCCCGGG GCCGGCTCGA GCTGGCCCTC 
TGCATGCTCC CGGGGCTGCC GGACACCGTC TTCGAGCTCA GCGAGGTGGA AGCCCTCCGG 
CTGGAGGCCA TCTGTGATAT CACCTTCCCC CCCGGCCTCT CGCAGCTGGT GCACCTGCAG 
GAGCTCAGCC TGCTCCACTC GCCTGCCAGG CTGCCCTTCT CGCTGCAGAT CTTCCTGCGG 
GACCGCCTGA AGGTCATCTG CGTCAAGTGC GAGGAGCTCC GCGACGTGCC CCTGTGGGTG 
TTTGGGCTGC GTGGCCTGGA GGAGCTGCAC CTCGAGGGGC TCTTCCCCCC GGAGCTGGCT 
CGGGCAGCGG CCCTAGAGAG CCTCCGGGAG CTGAAGCAGC TCAAGGCGCT GTCTCTGCGG 
AGCAATGCCA GCAAGGTGCC GGCCAGCGTG ACTGACGTGG CCGGGCACCT GCAGCGGCTT 
AGTCTGCACA ACGACGGGGC CCGCCTGCTG GCCCTGAACA GCCTTAAGAA GCTGGCGGCA 
CTGCGGGAGC TGGAGCTGGT GGCCTGCGGG CTGGAGCGCA TCCCCCATGC CATCTTCAGC 
CTGGGCGCGC TGCAGGAACT TGACCTCAGG GACAACCACC TGCGGTCCAT TGAAGAGATC 
CTCAGCTTCC AGCACTGTCG CAAGCTGGTC ACGCTCAAGC TGTGGCACAA CCAGATCGCT 
TACGTCCCTG AGCACGTGCG CAAGCTCCGG GGCCTGGAGC AGCTCTACCT CAGCCACAAC 
AAGCTGGAGA CCCTGCCCAC CCAGCTCGGC ATGTGCTCCG GCCTCCGCCT GCTGGACATC 
TCCCACAATG GGCTACGCTC CCTGCCGCCC GAGCTGGGCC TCCTCCAGAA CCTGCAGCAC 
CTGGCTCTGT CCTACAATGC CCTGGAGGCC CTGCCTGATG AGCTCTTCTT CTGCCGGAAG 
CTGCGGACGT TGCTTCTGGG CTACAACCAC CTGAGCCAGC TCTCGTCCCA GGTGGGGGCC 
CTCAGAGCCC TCAGCCGCCT GGAGCTCAAG GGCAACCGCT TGGAGGCACT ACCAGAAGAG 
CTTGGCAACT GTGTGGGGCT CAAGAAAGTG GGGATCCTCG TAGAAGACAC CCTTTACGAG 
GGCCTGCCGG CAGAGGTGCG GGAGAAACTG GAGGAGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005633001.1
CDS	346..2727
Translation	MIPVAEFKQFTEQQPAFKVLKPWWDVLAEYLTVAMLMIGVFGCTLQVTQDKIICLPSHEPRENLSEAPCQQLLPRGVSEQMAGLREVSGLKNNLDLQQYSFINQLCYETALHWYAKYFPYLVVIHTLIFMVCTSFWFKFPGTSSKIEHFISILGKCFDSPWTTRALSEVSGENHKGPAAGRVTATVEASAGPGKANEGEKEKVLVEPEKVVTEPPAVTLLDKKEGEQAKALFEKVKKFRVHVEEGDILYTMYIRQTVLKVCKFFAILVYNLVYVEKISFLVACRVETSEVTGYASFCCNHTKAHLFSKLAFCYISFVCVYGLTCLYTLYWLFHRPLKEYSFRSVREETGMGDIPDVKNDFAFMLHLIDQYDSLYSKRFAVFLSEVSESRLKQLNLNHEWTPEKLRQKLQRNARGRLELALCMLPGLPDTVFELSEVEALRLEAICDITFPPGLSQLVHLQELSLLHSPARLPFSLQIFLRDRLKVICVKCEELRDVPLWVFGLRGLEELHLEGLFPPELARAAALESLRELKQLKALSLRSNASKVPASVTDVAGHLQRLSLHNDGARLLALNSLKKLAALRELELVACGLERIPHAIFSLGALQELDLRDNHLRSIEEILSFQHCRKLVTLKLWHNQIAYVPEHVRKLRGLEQLYLSHNKLETLPTQLGMCSGLRLLDISHNGLRSLPPELGLLQNLQHLALSYNALEALPDELFFCRKLRTLLLGYNHLSQLSSQVGALRALSRLELKGNRLEALPEELGNCVGLKKVGILVEDTLYEGLPAEVREKLEEE

Target ORF information:

RefSeq Version	XM_005632944.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005632944.2

ORF Insert Sequence:

ATGATTCCGG TGGCCGAGTT CAAGCAGTTC ACGGAGCAGC AGCCCGCCTT CAAGGTGCTC 
AAACCCTGGT GGGACGTGCT GGCGGAGTAC CTCACCGTGG CTATGCTCAT GATCGGGGTC 
TTCGGCTGCA CCCTGCAGGT GACACAGGAC AAGATCATCT GTCTGCCCAG TCACGAACCC 
CGGGAGAATT TATCAGAGGC CCCATGCCAG CAACTGCTGC CACGGGGGGT CTCCGAGCAG 
ATGGCGGGCC TTCGGGAGGT AAGCGGCCTC AAGAACAACC TGGACCTGCA GCAGTACAGC 
TTCATTAACC AGCTCTGCTA CGAGACAGCC CTCCACTGGT ACGCCAAGTA CTTCCCCTAC 
CTGGTCGTCA TTCACACGCT CATCTTCATG GTCTGCACCA GCTTCTGGTT CAAGTTCCCT 
GGCACCAGCT CCAAGATCGA GCACTTCATC TCCATCCTGG GCAAATGTTT CGACTCGCCA 
TGGACCACAC GGGCCCTGTC TGAGGTCTCT GGGGAGAACC ATAAGGGCCC CGCCGCTGGA 
CGGGTGACGG CCACCGTGGA GGCCTCCGCG GGACCAGGAA AAGCTAATGA GGGTGAGAAG 
GAAAAGGTGC TGGTGGAGCC TGAGAAGGTG GTGACGGAGC CACCAGCCGT CACCCTGTTG 
GATAAGAAGG AGGGTGAACA GGCCAAAGCC CTGTTTGAGA AGGTCAAGAA GTTCCGTGTG 
CATGTGGAGG AGGGGGACAT CTTGTATACC ATGTACATCC GGCAAACGGT GCTCAAGGTC 
TGCAAGTTCT TTGCCATCCT GGTCTACAAC CTTGTCTACG TGGAGAAGAT CAGTTTCCTG 
GTGGCCTGCA GGGTAGAGAC GTCGGAGGTC ACGGGCTACG CCAGCTTCTG CTGCAACCAC 
ACCAAGGCCC ACCTCTTCTC CAAGCTGGCT TTCTGCTACA TCTCCTTCGT GTGCGTCTAT 
GGGCTAACCT GTCTCTACAC GCTCTACTGG CTCTTCCATC GGCCCCTCAA GGAGTACTCC 
TTCCGTTCTG TGCGGGAGGA GACCGGCATG GGTGACATAC CCGACGTCAA GAATGACTTT 
GCTTTCATGC TACATCTCAT CGACCAGTAT GACTCCCTCT ACTCCAAGCG CTTCGCCGTC 
TTCCTCTCCG AAGTCAGCGA GAGCCGCCTG AAGCAGCTCA ACCTGAACCA CGAGTGGACG 
CCTGAGAAGC TGCGGCAGAA GCTGCAGCGC AACGCCCGGG GCCGGCTCGA GCTGGCCCTC 
TGCATGCTCC CGGGGCTGCC GGACACCGTC TTCGAGCTCA GCGAGGTGGA AGCCCTCCGG 
CTGGAGGCCA TCTGTGATAT CACCTTCCCC CCCGGCCTCT CGCAGCTGGT GCACCTGCAG 
GAGCTCAGCC TGCTCCACTC GCCTGCCAGG CTGCCCTTCT CGCTGCAGAT CTTCCTGCGG 
GACCGCCTGA AGGTCATCTG CGTCAAGTGC GAGGAGCTCC GCGACGTGCC CCTGTGGGTG 
TTTGGGCTGC GTGGCCTGGA GGAGCTGCAC CTCGAGGGGC TCTTCCCCCC GGAGCTGGCT 
CGGGCAGCGG CCCTAGAGAG CCTCCGGGAG CTGAAGCAGC TCAAGGCGCT GTCTCTGCGG 
AGCAATGCCA GCAAGGTGCC GGCCAGCGTG ACTGACGTGG CCGGGCACCT GCAGCGGCTT 
AGTCTGCACA ACGACGGGGC CCGCCTGCTG GCCCTGAACA GCCTTAAGAA GCTGGCGGCA 
CTGCGGGAGC TGGAGCTGGT GGCCTGCGGG CTGGAGCGCA TCCCCCATGC CATCTTCAGC 
CTGGGCGCGC TGCAGGAACT TGACCTCAGG GACAACCACC TGCGGTCCAT TGAAGAGATC 
CTCAGCTTCC AGCACTGTCG CAAGCTGGTC ACGCTCAAGC TGTGGCACAA CCAGATCGCT 
TACGTCCCTG AGCACGTGCG CAAGCTCCGG GGCCTGGAGC AGCTCTACCT CAGCCACAAC 
AAGCTGGAGA CCCTGCCCAC CCAGCTCGGC ATGTGCTCCG GCCTCCGCCT GCTGGACATC 
TCCCACAATG GGCTACGCTC CCTGCCGCCC GAGCTGGGCC TCCTCCAGAA CCTGCAGCAC 
CTGGCTCTGT CCTACAATGC CCTGGAGGCC CTGCCTGATG AGCTCTTCTT CTGCCGGAAG 
CTGCGGACGT TGCTTCTGGG CTACAACCAC CTGAGCCAGC TCTCGTCCCA GGTGGGGGCC 
CTCAGAGCCC TCAGCCGCCT GGAGCTCAAG GGCAACCGCT TGGAGGCACT ACCAGAAGAG 
CTTGGCAACT GTGTGGGGCT CAAGAAAGTG GGGATCCTCG TAGAAGACAC CCTTTACGAG 
GGCCTGCCGG CAGAGGTGCG GGAGAAACTG GAGGAGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRC8E ( XM_005632944.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005633001.1 Canis lupus familiaris leucine rich repeat containing 8 family, member E (LRRC8E), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe22259
Clone ID Related Accession (Same CDS sequence)	XM_005632945.2 , XM_005632945.3 , XM_005632944.2 , XM_005632944.1 , XM_542121.5 , XM_542121.6
Accession Version	XM_005632944.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	volume-regulated anion channel subunit LRRC8E
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726096.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCCGG TGGCCGAGTT CAAGCAGTTC ACGGAGCAGC AGCCCGCCTT CAAGGTGCTC 
AAACCCTGGT GGGACGTGCT GGCGGAGTAC CTCACCGTGG CTATGCTCAT GATCGGGGTC 
TTCGGCTGCA CCCTGCAGGT GACACAGGAC AAGATCATCT GTCTGCCCAG TCACGAACCC 
CGGGAGAATT TATCAGAGGC CCCATGCCAG CAACTGCTGC CACGGGGGGT CTCCGAGCAG 
ATGGCGGGCC TTCGGGAGGT AAGCGGCCTC AAGAACAACC TGGACCTGCA GCAGTACAGC 
TTCATTAACC AGCTCTGCTA CGAGACAGCC CTCCACTGGT ACGCCAAGTA CTTCCCCTAC 
CTGGTCGTCA TTCACACGCT CATCTTCATG GTCTGCACCA GCTTCTGGTT CAAGTTCCCT 
GGCACCAGCT CCAAGATCGA GCACTTCATC TCCATCCTGG GCAAATGTTT CGACTCGCCA 
TGGACCACAC GGGCCCTGTC TGAGGTCTCT GGGGAGAACC ATAAGGGCCC CGCCGCTGGA 
CGGGTGACGG CCACCGTGGA GGCCTCCGCG GGACCAGGAA AAGCTAATGA GGGTGAGAAG 
GAAAAGGTGC TGGTGGAGCC TGAGAAGGTG GTGACGGAGC CACCAGCCGT CACCCTGTTG 
GATAAGAAGG AGGGTGAACA GGCCAAAGCC CTGTTTGAGA AGGTCAAGAA GTTCCGTGTG 
CATGTGGAGG AGGGGGACAT CTTGTATACC ATGTACATCC GGCAAACGGT GCTCAAGGTC 
TGCAAGTTCT TTGCCATCCT GGTCTACAAC CTTGTCTACG TGGAGAAGAT CAGTTTCCTG 
GTGGCCTGCA GGGTAGAGAC GTCGGAGGTC ACGGGCTACG CCAGCTTCTG CTGCAACCAC 
ACCAAGGCCC ACCTCTTCTC CAAGCTGGCT TTCTGCTACA TCTCCTTCGT GTGCGTCTAT 
GGGCTAACCT GTCTCTACAC GCTCTACTGG CTCTTCCATC GGCCCCTCAA GGAGTACTCC 
TTCCGTTCTG TGCGGGAGGA GACCGGCATG GGTGACATAC CCGACGTCAA GAATGACTTT 
GCTTTCATGC TACATCTCAT CGACCAGTAT GACTCCCTCT ACTCCAAGCG CTTCGCCGTC 
TTCCTCTCCG AAGTCAGCGA GAGCCGCCTG AAGCAGCTCA ACCTGAACCA CGAGTGGACG 
CCTGAGAAGC TGCGGCAGAA GCTGCAGCGC AACGCCCGGG GCCGGCTCGA GCTGGCCCTC 
TGCATGCTCC CGGGGCTGCC GGACACCGTC TTCGAGCTCA GCGAGGTGGA AGCCCTCCGG 
CTGGAGGCCA TCTGTGATAT CACCTTCCCC CCCGGCCTCT CGCAGCTGGT GCACCTGCAG 
GAGCTCAGCC TGCTCCACTC GCCTGCCAGG CTGCCCTTCT CGCTGCAGAT CTTCCTGCGG 
GACCGCCTGA AGGTCATCTG CGTCAAGTGC GAGGAGCTCC GCGACGTGCC CCTGTGGGTG 
TTTGGGCTGC GTGGCCTGGA GGAGCTGCAC CTCGAGGGGC TCTTCCCCCC GGAGCTGGCT 
CGGGCAGCGG CCCTAGAGAG CCTCCGGGAG CTGAAGCAGC TCAAGGCGCT GTCTCTGCGG 
AGCAATGCCA GCAAGGTGCC GGCCAGCGTG ACTGACGTGG CCGGGCACCT GCAGCGGCTT 
AGTCTGCACA ACGACGGGGC CCGCCTGCTG GCCCTGAACA GCCTTAAGAA GCTGGCGGCA 
CTGCGGGAGC TGGAGCTGGT GGCCTGCGGG CTGGAGCGCA TCCCCCATGC CATCTTCAGC 
CTGGGCGCGC TGCAGGAACT TGACCTCAGG GACAACCACC TGCGGTCCAT TGAAGAGATC 
CTCAGCTTCC AGCACTGTCG CAAGCTGGTC ACGCTCAAGC TGTGGCACAA CCAGATCGCT 
TACGTCCCTG AGCACGTGCG CAAGCTCCGG GGCCTGGAGC AGCTCTACCT CAGCCACAAC 
AAGCTGGAGA CCCTGCCCAC CCAGCTCGGC ATGTGCTCCG GCCTCCGCCT GCTGGACATC 
TCCCACAATG GGCTACGCTC CCTGCCGCCC GAGCTGGGCC TCCTCCAGAA CCTGCAGCAC 
CTGGCTCTGT CCTACAATGC CCTGGAGGCC CTGCCTGATG AGCTCTTCTT CTGCCGGAAG 
CTGCGGACGT TGCTTCTGGG CTACAACCAC CTGAGCCAGC TCTCGTCCCA GGTGGGGGCC 
CTCAGAGCCC TCAGCCGCCT GGAGCTCAAG GGCAACCGCT TGGAGGCACT ACCAGAAGAG 
CTTGGCAACT GTGTGGGGCT CAAGAAAGTG GGGATCCTCG TAGAAGACAC CCTTTACGAG 
GGCCTGCCGG CAGAGGTGCG GGAGAAACTG GAGGAGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005633001.1
CDS	51..2432
Translation	MIPVAEFKQFTEQQPAFKVLKPWWDVLAEYLTVAMLMIGVFGCTLQVTQDKIICLPSHEPRENLSEAPCQQLLPRGVSEQMAGLREVSGLKNNLDLQQYSFINQLCYETALHWYAKYFPYLVVIHTLIFMVCTSFWFKFPGTSSKIEHFISILGKCFDSPWTTRALSEVSGENHKGPAAGRVTATVEASAGPGKANEGEKEKVLVEPEKVVTEPPAVTLLDKKEGEQAKALFEKVKKFRVHVEEGDILYTMYIRQTVLKVCKFFAILVYNLVYVEKISFLVACRVETSEVTGYASFCCNHTKAHLFSKLAFCYISFVCVYGLTCLYTLYWLFHRPLKEYSFRSVREETGMGDIPDVKNDFAFMLHLIDQYDSLYSKRFAVFLSEVSESRLKQLNLNHEWTPEKLRQKLQRNARGRLELALCMLPGLPDTVFELSEVEALRLEAICDITFPPGLSQLVHLQELSLLHSPARLPFSLQIFLRDRLKVICVKCEELRDVPLWVFGLRGLEELHLEGLFPPELARAAALESLRELKQLKALSLRSNASKVPASVTDVAGHLQRLSLHNDGARLLALNSLKKLAALRELELVACGLERIPHAIFSLGALQELDLRDNHLRSIEEILSFQHCRKLVTLKLWHNQIAYVPEHVRKLRGLEQLYLSHNKLETLPTQLGMCSGLRLLDISHNGLRSLPPELGLLQNLQHLALSYNALEALPDELFFCRKLRTLLLGYNHLSQLSSQVGALRALSRLELKGNRLEALPEELGNCVGLKKVGILVEDTLYEGLPAEVREKLEEE

Target ORF information:

RefSeq Version	XM_005632944.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris leucine rich repeat containing 8 family, member E (LRRC8E), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005632944.1

ORF Insert Sequence:

ATGATTCCGG TGGCCGAGTT CAAGCAGTTC ACGGAGCAGC AGCCCGCCTT CAAGGTGCTC 
AAACCCTGGT GGGACGTGCT GGCGGAGTAC CTCACCGTGG CTATGCTCAT GATCGGGGTC 
TTCGGCTGCA CCCTGCAGGT GACACAGGAC AAGATCATCT GTCTGCCCAG TCACGAACCC 
CGGGAGAATT TATCAGAGGC CCCATGCCAG CAACTGCTGC CACGGGGGGT CTCCGAGCAG 
ATGGCGGGCC TTCGGGAGGT AAGCGGCCTC AAGAACAACC TGGACCTGCA GCAGTACAGC 
TTCATTAACC AGCTCTGCTA CGAGACAGCC CTCCACTGGT ACGCCAAGTA CTTCCCCTAC 
CTGGTCGTCA TTCACACGCT CATCTTCATG GTCTGCACCA GCTTCTGGTT CAAGTTCCCT 
GGCACCAGCT CCAAGATCGA GCACTTCATC TCCATCCTGG GCAAATGTTT CGACTCGCCA 
TGGACCACAC GGGCCCTGTC TGAGGTCTCT GGGGAGAACC ATAAGGGCCC CGCCGCTGGA 
CGGGTGACGG CCACCGTGGA GGCCTCCGCG GGACCAGGAA AAGCTAATGA GGGTGAGAAG 
GAAAAGGTGC TGGTGGAGCC TGAGAAGGTG GTGACGGAGC CACCAGCCGT CACCCTGTTG 
GATAAGAAGG AGGGTGAACA GGCCAAAGCC CTGTTTGAGA AGGTCAAGAA GTTCCGTGTG 
CATGTGGAGG AGGGGGACAT CTTGTATACC ATGTACATCC GGCAAACGGT GCTCAAGGTC 
TGCAAGTTCT TTGCCATCCT GGTCTACAAC CTTGTCTACG TGGAGAAGAT CAGTTTCCTG 
GTGGCCTGCA GGGTAGAGAC GTCGGAGGTC ACGGGCTACG CCAGCTTCTG CTGCAACCAC 
ACCAAGGCCC ACCTCTTCTC CAAGCTGGCT TTCTGCTACA TCTCCTTCGT GTGCGTCTAT 
GGGCTAACCT GTCTCTACAC GCTCTACTGG CTCTTCCATC GGCCCCTCAA GGAGTACTCC 
TTCCGTTCTG TGCGGGAGGA GACCGGCATG GGTGACATAC CCGACGTCAA GAATGACTTT 
GCTTTCATGC TACATCTCAT CGACCAGTAT GACTCCCTCT ACTCCAAGCG CTTCGCCGTC 
TTCCTCTCCG AAGTCAGCGA GAGCCGCCTG AAGCAGCTCA ACCTGAACCA CGAGTGGACG 
CCTGAGAAGC TGCGGCAGAA GCTGCAGCGC AACGCCCGGG GCCGGCTCGA GCTGGCCCTC 
TGCATGCTCC CGGGGCTGCC GGACACCGTC TTCGAGCTCA GCGAGGTGGA AGCCCTCCGG 
CTGGAGGCCA TCTGTGATAT CACCTTCCCC CCCGGCCTCT CGCAGCTGGT GCACCTGCAG 
GAGCTCAGCC TGCTCCACTC GCCTGCCAGG CTGCCCTTCT CGCTGCAGAT CTTCCTGCGG 
GACCGCCTGA AGGTCATCTG CGTCAAGTGC GAGGAGCTCC GCGACGTGCC CCTGTGGGTG 
TTTGGGCTGC GTGGCCTGGA GGAGCTGCAC CTCGAGGGGC TCTTCCCCCC GGAGCTGGCT 
CGGGCAGCGG CCCTAGAGAG CCTCCGGGAG CTGAAGCAGC TCAAGGCGCT GTCTCTGCGG 
AGCAATGCCA GCAAGGTGCC GGCCAGCGTG ACTGACGTGG CCGGGCACCT GCAGCGGCTT 
AGTCTGCACA ACGACGGGGC CCGCCTGCTG GCCCTGAACA GCCTTAAGAA GCTGGCGGCA 
CTGCGGGAGC TGGAGCTGGT GGCCTGCGGG CTGGAGCGCA TCCCCCATGC CATCTTCAGC 
CTGGGCGCGC TGCAGGAACT TGACCTCAGG GACAACCACC TGCGGTCCAT TGAAGAGATC 
CTCAGCTTCC AGCACTGTCG CAAGCTGGTC ACGCTCAAGC TGTGGCACAA CCAGATCGCT 
TACGTCCCTG AGCACGTGCG CAAGCTCCGG GGCCTGGAGC AGCTCTACCT CAGCCACAAC 
AAGCTGGAGA CCCTGCCCAC CCAGCTCGGC ATGTGCTCCG GCCTCCGCCT GCTGGACATC 
TCCCACAATG GGCTACGCTC CCTGCCGCCC GAGCTGGGCC TCCTCCAGAA CCTGCAGCAC 
CTGGCTCTGT CCTACAATGC CCTGGAGGCC CTGCCTGATG AGCTCTTCTT CTGCCGGAAG 
CTGCGGACGT TGCTTCTGGG CTACAACCAC CTGAGCCAGC TCTCGTCCCA GGTGGGGGCC 
CTCAGAGCCC TCAGCCGCCT GGAGCTCAAG GGCAACCGCT TGGAGGCACT ACCAGAAGAG 
CTTGGCAACT GTGTGGGGCT CAAGAAAGTG GGGATCCTCG TAGAAGACAC CCTTTACGAG 
GGCCTGCCGG CAGAGGTGCG GGAGAAACTG GAGGAGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRC8E ( XM_542121.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_542121.2 Canis lupus familiaris leucine rich repeat containing 8 family, member E (LRRC8E), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe22259
Clone ID Related Accession (Same CDS sequence)	XM_005632945.2 , XM_005632945.3 , XM_005632944.2 , XM_005632944.1 , XM_542121.5 , XM_542121.6
Accession Version	XM_542121.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	volume-regulated anion channel subunit LRRC8E
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726096.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_542121.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCCGG TGGCCGAGTT CAAGCAGTTC ACGGAGCAGC AGCCCGCCTT CAAGGTGCTC 
AAACCCTGGT GGGACGTGCT GGCGGAGTAC CTCACCGTGG CTATGCTCAT GATCGGGGTC 
TTCGGCTGCA CCCTGCAGGT GACACAGGAC AAGATCATCT GTCTGCCCAG TCACGAACCC 
CGGGAGAATT TATCAGAGGC CCCATGCCAG CAACTGCTGC CACGGGGGGT CTCCGAGCAG 
ATGGCGGGCC TTCGGGAGGT AAGCGGCCTC AAGAACAACC TGGACCTGCA GCAGTACAGC 
TTCATTAACC AGCTCTGCTA CGAGACAGCC CTCCACTGGT ACGCCAAGTA CTTCCCCTAC 
CTGGTCGTCA TTCACACGCT CATCTTCATG GTCTGCACCA GCTTCTGGTT CAAGTTCCCT 
GGCACCAGCT CCAAGATCGA GCACTTCATC TCCATCCTGG GCAAATGTTT CGACTCGCCA 
TGGACCACAC GGGCCCTGTC TGAGGTCTCT GGGGAGAACC ATAAGGGCCC CGCCGCTGGA 
CGGGTGACGG CCACCGTGGA GGCCTCCGCG GGACCAGGAA AAGCTAATGA GGGTGAGAAG 
GAAAAGGTGC TGGTGGAGCC TGAGAAGGTG GTGACGGAGC CACCAGCCGT CACCCTGTTG 
GATAAGAAGG AGGGTGAACA GGCCAAAGCC CTGTTTGAGA AGGTCAAGAA GTTCCGTGTG 
CATGTGGAGG AGGGGGACAT CTTGTATACC ATGTACATCC GGCAAACGGT GCTCAAGGTC 
TGCAAGTTCT TTGCCATCCT GGTCTACAAC CTTGTCTACG TGGAGAAGAT CAGTTTCCTG 
GTGGCCTGCA GGGTAGAGAC GTCGGAGGTC ACGGGCTACG CCAGCTTCTG CTGCAACCAC 
ACCAAGGCCC ACCTCTTCTC CAAGCTGGCT TTCTGCTACA TCTCCTTCGT GTGCGTCTAT 
GGGCTAACCT GTCTCTACAC GCTCTACTGG CTCTTCCATC GGCCCCTCAA GGAGTACTCC 
TTCCGTTCTG TGCGGGAGGA GACCGGCATG GGTGACATAC CCGACGTCAA GAATGACTTT 
GCTTTCATGC TACATCTCAT CGACCAGTAT GACTCCCTCT ACTCCAAGCG CTTCGCCGTC 
TTCCTCTCCG AAGTCAGCGA GAGCCGCCTG AAGCAGCTCA ACCTGAACCA CGAGTGGACG 
CCTGAGAAGC TGCGGCAGAA GCTGCAGCGC AACGCCCGGG GCCGGCTCGA GCTGGCCCTC 
TGCATGCTCC CGGGGCTGCC GGACACCGTC TTCGAGCTCA GCGAGGTGGA AGCCCTCCGG 
CTGGAGGCCA TCTGTGATAT CACCTTCCCC CCCGGCCTCT CGCAGCTGGT GCACCTGCAG 
GAGCTCAGCC TGCTCCACTC GCCTGCCAGG CTGCCCTTCT CGCTGCAGAT CTTCCTGCGG 
GACCGCCTGA AGGTCATCTG CGTCAAGTGC GAGGAGCTCC GCGACGTGCC CCTGTGGGTG 
TTTGGGCTGC GTGGCCTGGA GGAGCTGCAC CTCGAGGGGC TCTTCCCCCC GGAGCTGGCT 
CGGGCAGCGG CCCTAGAGAG CCTCCGGGAG CTGAAGCAGC TCAAGGCGCT GTCTCTGCGG 
AGCAATGCCA GCAAGGTGCC GGCCAGCGTG ACTGACGTGG CCGGGCACCT GCAGCGGCTT 
AGTCTGCACA ACGACGGGGC CCGCCTGCTG GCCCTGAACA GCCTTAAGAA GCTGGCGGCA 
CTGCGGGAGC TGGAGCTGGT GGCCTGCGGG CTGGAGCGCA TCCCCCATGC CATCTTCAGC 
CTGGGCGCGC TGCAGGAACT TGACCTCAGG GACAACCACC TGCGGTCCAT TGAAGAGATC 
CTCAGCTTCC AGCACTGTCG CAAGCTGGTC ACGCTCAAGC TGTGGCACAA CCAGATCGCT 
TACGTCCCTG AGCACGTGCG CAAGCTCCGG GGCCTGGAGC AGCTCTACCT CAGCCACAAC 
AAGCTGGAGA CCCTGCCCAC CCAGCTCGGC ATGTGCTCCG GCCTCCGCCT GCTGGACATC 
TCCCACAATG GGCTACGCTC CCTGCCGCCC GAGCTGGGCC TCCTCCAGAA CCTGCAGCAC 
CTGGCTCTGT CCTACAATGC CCTGGAGGCC CTGCCTGATG AGCTCTTCTT CTGCCGGAAG 
CTGCGGACGT TGCTTCTGGG CTACAACCAC CTGAGCCAGC TCTCGTCCCA GGTGGGGGCC 
CTCAGAGCCC TCAGCCGCCT GGAGCTCAAG GGCAACCGCT TGGAGGCACT ACCAGAAGAG 
CTTGGCAACT GTGTGGGGCT CAAGAAAGTG GGGATCCTCG TAGAAGACAC CCTTTACGAG 
GGCCTGCCGG CAGAGGTGCG GGAGAAACTG GAGGAGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_542121.2
CDS	74..2455
Translation	MIPVAEFKQFTEQQPAFKVLKPWWDVLAEYLTVAMLMIGVFGCTLQVTQDKIICLPSHEPRENLSEAPCQQLLPRGVSEQMAGLREVSGLKNNLDLQQYSFINQLCYETALHWYAKYFPYLVVIHTLIFMVCTSFWFKFPGTSSKIEHFISILGKCFDSPWTTRALSEVSGENHKGPAAGRVTATVEASAGPGKANEGEKEKVLVEPEKVVTEPPAVTLLDKKEGEQAKALFEKVKKFRVHVEEGDILYTMYIRQTVLKVCKFFAILVYNLVYVEKISFLVACRVETSEVTGYASFCCNHTKAHLFSKLAFCYISFVCVYGLTCLYTLYWLFHRPLKEYSFRSVREETGMGDIPDVKNDFAFMLHLIDQYDSLYSKRFAVFLSEVSESRLKQLNLNHEWTPEKLRQKLQRNARGRLELALCMLPGLPDTVFELSEVEALRLEAICDITFPPGLSQLVHLQELSLLHSPARLPFSLQIFLRDRLKVICVKCEELRDVPLWVFGLRGLEELHLEGLFPPELARAAALESLRELKQLKALSLRSNASKVPASVTDVAGHLQRLSLHNDGARLLALNSLKKLAALRELELVACGLERIPHAIFSLGALQELDLRDNHLRSIEEILSFQHCRKLVTLKLWHNQIAYVPEHVRKLRGLEQLYLSHNKLETLPTQLGMCSGLRLLDISHNGLRSLPPELGLLQNLQHLALSYNALEALPDELFFCRKLRTLLLGYNHLSQLSSQVGALRALSRLELKGNRLEALPEELGNCVGLKKVGILVEDTLYEGLPAEVREKLEEE

Target ORF information:

RefSeq Version	XM_542121.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris leucine rich repeat containing 8 family, member E (LRRC8E), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_542121.5

ORF Insert Sequence:

ATGATTCCGG TGGCCGAGTT CAAGCAGTTC ACGGAGCAGC AGCCCGCCTT CAAGGTGCTC 
AAACCCTGGT GGGACGTGCT GGCGGAGTAC CTCACCGTGG CTATGCTCAT GATCGGGGTC 
TTCGGCTGCA CCCTGCAGGT GACACAGGAC AAGATCATCT GTCTGCCCAG TCACGAACCC 
CGGGAGAATT TATCAGAGGC CCCATGCCAG CAACTGCTGC CACGGGGGGT CTCCGAGCAG 
ATGGCGGGCC TTCGGGAGGT AAGCGGCCTC AAGAACAACC TGGACCTGCA GCAGTACAGC 
TTCATTAACC AGCTCTGCTA CGAGACAGCC CTCCACTGGT ACGCCAAGTA CTTCCCCTAC 
CTGGTCGTCA TTCACACGCT CATCTTCATG GTCTGCACCA GCTTCTGGTT CAAGTTCCCT 
GGCACCAGCT CCAAGATCGA GCACTTCATC TCCATCCTGG GCAAATGTTT CGACTCGCCA 
TGGACCACAC GGGCCCTGTC TGAGGTCTCT GGGGAGAACC ATAAGGGCCC CGCCGCTGGA 
CGGGTGACGG CCACCGTGGA GGCCTCCGCG GGACCAGGAA AAGCTAATGA GGGTGAGAAG 
GAAAAGGTGC TGGTGGAGCC TGAGAAGGTG GTGACGGAGC CACCAGCCGT CACCCTGTTG 
GATAAGAAGG AGGGTGAACA GGCCAAAGCC CTGTTTGAGA AGGTCAAGAA GTTCCGTGTG 
CATGTGGAGG AGGGGGACAT CTTGTATACC ATGTACATCC GGCAAACGGT GCTCAAGGTC 
TGCAAGTTCT TTGCCATCCT GGTCTACAAC CTTGTCTACG TGGAGAAGAT CAGTTTCCTG 
GTGGCCTGCA GGGTAGAGAC GTCGGAGGTC ACGGGCTACG CCAGCTTCTG CTGCAACCAC 
ACCAAGGCCC ACCTCTTCTC CAAGCTGGCT TTCTGCTACA TCTCCTTCGT GTGCGTCTAT 
GGGCTAACCT GTCTCTACAC GCTCTACTGG CTCTTCCATC GGCCCCTCAA GGAGTACTCC 
TTCCGTTCTG TGCGGGAGGA GACCGGCATG GGTGACATAC CCGACGTCAA GAATGACTTT 
GCTTTCATGC TACATCTCAT CGACCAGTAT GACTCCCTCT ACTCCAAGCG CTTCGCCGTC 
TTCCTCTCCG AAGTCAGCGA GAGCCGCCTG AAGCAGCTCA ACCTGAACCA CGAGTGGACG 
CCTGAGAAGC TGCGGCAGAA GCTGCAGCGC AACGCCCGGG GCCGGCTCGA GCTGGCCCTC 
TGCATGCTCC CGGGGCTGCC GGACACCGTC TTCGAGCTCA GCGAGGTGGA AGCCCTCCGG 
CTGGAGGCCA TCTGTGATAT CACCTTCCCC CCCGGCCTCT CGCAGCTGGT GCACCTGCAG 
GAGCTCAGCC TGCTCCACTC GCCTGCCAGG CTGCCCTTCT CGCTGCAGAT CTTCCTGCGG 
GACCGCCTGA AGGTCATCTG CGTCAAGTGC GAGGAGCTCC GCGACGTGCC CCTGTGGGTG 
TTTGGGCTGC GTGGCCTGGA GGAGCTGCAC CTCGAGGGGC TCTTCCCCCC GGAGCTGGCT 
CGGGCAGCGG CCCTAGAGAG CCTCCGGGAG CTGAAGCAGC TCAAGGCGCT GTCTCTGCGG 
AGCAATGCCA GCAAGGTGCC GGCCAGCGTG ACTGACGTGG CCGGGCACCT GCAGCGGCTT 
AGTCTGCACA ACGACGGGGC CCGCCTGCTG GCCCTGAACA GCCTTAAGAA GCTGGCGGCA 
CTGCGGGAGC TGGAGCTGGT GGCCTGCGGG CTGGAGCGCA TCCCCCATGC CATCTTCAGC 
CTGGGCGCGC TGCAGGAACT TGACCTCAGG GACAACCACC TGCGGTCCAT TGAAGAGATC 
CTCAGCTTCC AGCACTGTCG CAAGCTGGTC ACGCTCAAGC TGTGGCACAA CCAGATCGCT 
TACGTCCCTG AGCACGTGCG CAAGCTCCGG GGCCTGGAGC AGCTCTACCT CAGCCACAAC 
AAGCTGGAGA CCCTGCCCAC CCAGCTCGGC ATGTGCTCCG GCCTCCGCCT GCTGGACATC 
TCCCACAATG GGCTACGCTC CCTGCCGCCC GAGCTGGGCC TCCTCCAGAA CCTGCAGCAC 
CTGGCTCTGT CCTACAATGC CCTGGAGGCC CTGCCTGATG AGCTCTTCTT CTGCCGGAAG 
CTGCGGACGT TGCTTCTGGG CTACAACCAC CTGAGCCAGC TCTCGTCCCA GGTGGGGGCC 
CTCAGAGCCC TCAGCCGCCT GGAGCTCAAG GGCAACCGCT TGGAGGCACT ACCAGAAGAG 
CTTGGCAACT GTGTGGGGCT CAAGAAAGTG GGGATCCTCG TAGAAGACAC CCTTTACGAG 
GGCCTGCCGG CAGAGGTGCG GGAGAAACTG GAGGAGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRC8E ( XM_542121.6 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_542121.2 Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant X1, mRNA.

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CloneID	OCe22259
Clone ID Related Accession (Same CDS sequence)	XM_005632945.2 , XM_005632945.3 , XM_005632944.2 , XM_005632944.1 , XM_542121.5 , XM_542121.6
Accession Version	XM_542121.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	volume-regulated anion channel subunit LRRC8E
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_542121.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCCGG TGGCCGAGTT CAAGCAGTTC ACGGAGCAGC AGCCCGCCTT CAAGGTGCTC 
AAACCCTGGT GGGACGTGCT GGCGGAGTAC CTCACCGTGG CTATGCTCAT GATCGGGGTC 
TTCGGCTGCA CCCTGCAGGT GACACAGGAC AAGATCATCT GTCTGCCCAG TCACGAACCC 
CGGGAGAATT TATCAGAGGC CCCATGCCAG CAACTGCTGC CACGGGGGGT CTCCGAGCAG 
ATGGCGGGCC TTCGGGAGGT AAGCGGCCTC AAGAACAACC TGGACCTGCA GCAGTACAGC 
TTCATTAACC AGCTCTGCTA CGAGACAGCC CTCCACTGGT ACGCCAAGTA CTTCCCCTAC 
CTGGTCGTCA TTCACACGCT CATCTTCATG GTCTGCACCA GCTTCTGGTT CAAGTTCCCT 
GGCACCAGCT CCAAGATCGA GCACTTCATC TCCATCCTGG GCAAATGTTT CGACTCGCCA 
TGGACCACAC GGGCCCTGTC TGAGGTCTCT GGGGAGAACC ATAAGGGCCC CGCCGCTGGA 
CGGGTGACGG CCACCGTGGA GGCCTCCGCG GGACCAGGAA AAGCTAATGA GGGTGAGAAG 
GAAAAGGTGC TGGTGGAGCC TGAGAAGGTG GTGACGGAGC CACCAGCCGT CACCCTGTTG 
GATAAGAAGG AGGGTGAACA GGCCAAAGCC CTGTTTGAGA AGGTCAAGAA GTTCCGTGTG 
CATGTGGAGG AGGGGGACAT CTTGTATACC ATGTACATCC GGCAAACGGT GCTCAAGGTC 
TGCAAGTTCT TTGCCATCCT GGTCTACAAC CTTGTCTACG TGGAGAAGAT CAGTTTCCTG 
GTGGCCTGCA GGGTAGAGAC GTCGGAGGTC ACGGGCTACG CCAGCTTCTG CTGCAACCAC 
ACCAAGGCCC ACCTCTTCTC CAAGCTGGCT TTCTGCTACA TCTCCTTCGT GTGCGTCTAT 
GGGCTAACCT GTCTCTACAC GCTCTACTGG CTCTTCCATC GGCCCCTCAA GGAGTACTCC 
TTCCGTTCTG TGCGGGAGGA GACCGGCATG GGTGACATAC CCGACGTCAA GAATGACTTT 
GCTTTCATGC TACATCTCAT CGACCAGTAT GACTCCCTCT ACTCCAAGCG CTTCGCCGTC 
TTCCTCTCCG AAGTCAGCGA GAGCCGCCTG AAGCAGCTCA ACCTGAACCA CGAGTGGACG 
CCTGAGAAGC TGCGGCAGAA GCTGCAGCGC AACGCCCGGG GCCGGCTCGA GCTGGCCCTC 
TGCATGCTCC CGGGGCTGCC GGACACCGTC TTCGAGCTCA GCGAGGTGGA AGCCCTCCGG 
CTGGAGGCCA TCTGTGATAT CACCTTCCCC CCCGGCCTCT CGCAGCTGGT GCACCTGCAG 
GAGCTCAGCC TGCTCCACTC GCCTGCCAGG CTGCCCTTCT CGCTGCAGAT CTTCCTGCGG 
GACCGCCTGA AGGTCATCTG CGTCAAGTGC GAGGAGCTCC GCGACGTGCC CCTGTGGGTG 
TTTGGGCTGC GTGGCCTGGA GGAGCTGCAC CTCGAGGGGC TCTTCCCCCC GGAGCTGGCT 
CGGGCAGCGG CCCTAGAGAG CCTCCGGGAG CTGAAGCAGC TCAAGGCGCT GTCTCTGCGG 
AGCAATGCCA GCAAGGTGCC GGCCAGCGTG ACTGACGTGG CCGGGCACCT GCAGCGGCTT 
AGTCTGCACA ACGACGGGGC CCGCCTGCTG GCCCTGAACA GCCTTAAGAA GCTGGCGGCA 
CTGCGGGAGC TGGAGCTGGT GGCCTGCGGG CTGGAGCGCA TCCCCCATGC CATCTTCAGC 
CTGGGCGCGC TGCAGGAACT TGACCTCAGG GACAACCACC TGCGGTCCAT TGAAGAGATC 
CTCAGCTTCC AGCACTGTCG CAAGCTGGTC ACGCTCAAGC TGTGGCACAA CCAGATCGCT 
TACGTCCCTG AGCACGTGCG CAAGCTCCGG GGCCTGGAGC AGCTCTACCT CAGCCACAAC 
AAGCTGGAGA CCCTGCCCAC CCAGCTCGGC ATGTGCTCCG GCCTCCGCCT GCTGGACATC 
TCCCACAATG GGCTACGCTC CCTGCCGCCC GAGCTGGGCC TCCTCCAGAA CCTGCAGCAC 
CTGGCTCTGT CCTACAATGC CCTGGAGGCC CTGCCTGATG AGCTCTTCTT CTGCCGGAAG 
CTGCGGACGT TGCTTCTGGG CTACAACCAC CTGAGCCAGC TCTCGTCCCA GGTGGGGGCC 
CTCAGAGCCC TCAGCCGCCT GGAGCTCAAG GGCAACCGCT TGGAGGCACT ACCAGAAGAG 
CTTGGCAACT GTGTGGGGCT CAAGAAAGTG GGGATCCTCG TAGAAGACAC CCTTTACGAG 
GGCCTGCCGG CAGAGGTGCG GGAGAAACTG GAGGAGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_542121.2
CDS	91..2472
Translation	MIPVAEFKQFTEQQPAFKVLKPWWDVLAEYLTVAMLMIGVFGCTLQVTQDKIICLPSHEPRENLSEAPCQQLLPRGVSEQMAGLREVSGLKNNLDLQQYSFINQLCYETALHWYAKYFPYLVVIHTLIFMVCTSFWFKFPGTSSKIEHFISILGKCFDSPWTTRALSEVSGENHKGPAAGRVTATVEASAGPGKANEGEKEKVLVEPEKVVTEPPAVTLLDKKEGEQAKALFEKVKKFRVHVEEGDILYTMYIRQTVLKVCKFFAILVYNLVYVEKISFLVACRVETSEVTGYASFCCNHTKAHLFSKLAFCYISFVCVYGLTCLYTLYWLFHRPLKEYSFRSVREETGMGDIPDVKNDFAFMLHLIDQYDSLYSKRFAVFLSEVSESRLKQLNLNHEWTPEKLRQKLQRNARGRLELALCMLPGLPDTVFELSEVEALRLEAICDITFPPGLSQLVHLQELSLLHSPARLPFSLQIFLRDRLKVICVKCEELRDVPLWVFGLRGLEELHLEGLFPPELARAAALESLRELKQLKALSLRSNASKVPASVTDVAGHLQRLSLHNDGARLLALNSLKKLAALRELELVACGLERIPHAIFSLGALQELDLRDNHLRSIEEILSFQHCRKLVTLKLWHNQIAYVPEHVRKLRGLEQLYLSHNKLETLPTQLGMCSGLRLLDISHNGLRSLPPELGLLQNLQHLALSYNALEALPDELFFCRKLRTLLLGYNHLSQLSSQVGALRALSRLELKGNRLEALPEELGNCVGLKKVGILVEDTLYEGLPAEVREKLEEE

Target ORF information:

RefSeq Version	XM_542121.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit E (LRRC8E), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_542121.6

ORF Insert Sequence:

ATGATTCCGG TGGCCGAGTT CAAGCAGTTC ACGGAGCAGC AGCCCGCCTT CAAGGTGCTC 
AAACCCTGGT GGGACGTGCT GGCGGAGTAC CTCACCGTGG CTATGCTCAT GATCGGGGTC 
TTCGGCTGCA CCCTGCAGGT GACACAGGAC AAGATCATCT GTCTGCCCAG TCACGAACCC 
CGGGAGAATT TATCAGAGGC CCCATGCCAG CAACTGCTGC CACGGGGGGT CTCCGAGCAG 
ATGGCGGGCC TTCGGGAGGT AAGCGGCCTC AAGAACAACC TGGACCTGCA GCAGTACAGC 
TTCATTAACC AGCTCTGCTA CGAGACAGCC CTCCACTGGT ACGCCAAGTA CTTCCCCTAC 
CTGGTCGTCA TTCACACGCT CATCTTCATG GTCTGCACCA GCTTCTGGTT CAAGTTCCCT 
GGCACCAGCT CCAAGATCGA GCACTTCATC TCCATCCTGG GCAAATGTTT CGACTCGCCA 
TGGACCACAC GGGCCCTGTC TGAGGTCTCT GGGGAGAACC ATAAGGGCCC CGCCGCTGGA 
CGGGTGACGG CCACCGTGGA GGCCTCCGCG GGACCAGGAA AAGCTAATGA GGGTGAGAAG 
GAAAAGGTGC TGGTGGAGCC TGAGAAGGTG GTGACGGAGC CACCAGCCGT CACCCTGTTG 
GATAAGAAGG AGGGTGAACA GGCCAAAGCC CTGTTTGAGA AGGTCAAGAA GTTCCGTGTG 
CATGTGGAGG AGGGGGACAT CTTGTATACC ATGTACATCC GGCAAACGGT GCTCAAGGTC 
TGCAAGTTCT TTGCCATCCT GGTCTACAAC CTTGTCTACG TGGAGAAGAT CAGTTTCCTG 
GTGGCCTGCA GGGTAGAGAC GTCGGAGGTC ACGGGCTACG CCAGCTTCTG CTGCAACCAC 
ACCAAGGCCC ACCTCTTCTC CAAGCTGGCT TTCTGCTACA TCTCCTTCGT GTGCGTCTAT 
GGGCTAACCT GTCTCTACAC GCTCTACTGG CTCTTCCATC GGCCCCTCAA GGAGTACTCC 
TTCCGTTCTG TGCGGGAGGA GACCGGCATG GGTGACATAC CCGACGTCAA GAATGACTTT 
GCTTTCATGC TACATCTCAT CGACCAGTAT GACTCCCTCT ACTCCAAGCG CTTCGCCGTC 
TTCCTCTCCG AAGTCAGCGA GAGCCGCCTG AAGCAGCTCA ACCTGAACCA CGAGTGGACG 
CCTGAGAAGC TGCGGCAGAA GCTGCAGCGC AACGCCCGGG GCCGGCTCGA GCTGGCCCTC 
TGCATGCTCC CGGGGCTGCC GGACACCGTC TTCGAGCTCA GCGAGGTGGA AGCCCTCCGG 
CTGGAGGCCA TCTGTGATAT CACCTTCCCC CCCGGCCTCT CGCAGCTGGT GCACCTGCAG 
GAGCTCAGCC TGCTCCACTC GCCTGCCAGG CTGCCCTTCT CGCTGCAGAT CTTCCTGCGG 
GACCGCCTGA AGGTCATCTG CGTCAAGTGC GAGGAGCTCC GCGACGTGCC CCTGTGGGTG 
TTTGGGCTGC GTGGCCTGGA GGAGCTGCAC CTCGAGGGGC TCTTCCCCCC GGAGCTGGCT 
CGGGCAGCGG CCCTAGAGAG CCTCCGGGAG CTGAAGCAGC TCAAGGCGCT GTCTCTGCGG 
AGCAATGCCA GCAAGGTGCC GGCCAGCGTG ACTGACGTGG CCGGGCACCT GCAGCGGCTT 
AGTCTGCACA ACGACGGGGC CCGCCTGCTG GCCCTGAACA GCCTTAAGAA GCTGGCGGCA 
CTGCGGGAGC TGGAGCTGGT GGCCTGCGGG CTGGAGCGCA TCCCCCATGC CATCTTCAGC 
CTGGGCGCGC TGCAGGAACT TGACCTCAGG GACAACCACC TGCGGTCCAT TGAAGAGATC 
CTCAGCTTCC AGCACTGTCG CAAGCTGGTC ACGCTCAAGC TGTGGCACAA CCAGATCGCT 
TACGTCCCTG AGCACGTGCG CAAGCTCCGG GGCCTGGAGC AGCTCTACCT CAGCCACAAC 
AAGCTGGAGA CCCTGCCCAC CCAGCTCGGC ATGTGCTCCG GCCTCCGCCT GCTGGACATC 
TCCCACAATG GGCTACGCTC CCTGCCGCCC GAGCTGGGCC TCCTCCAGAA CCTGCAGCAC 
CTGGCTCTGT CCTACAATGC CCTGGAGGCC CTGCCTGATG AGCTCTTCTT CTGCCGGAAG 
CTGCGGACGT TGCTTCTGGG CTACAACCAC CTGAGCCAGC TCTCGTCCCA GGTGGGGGCC 
CTCAGAGCCC TCAGCCGCCT GGAGCTCAAG GGCAACCGCT TGGAGGCACT ACCAGAAGAG 
CTTGGCAACT GTGTGGGGCT CAAGAAAGTG GGGATCCTCG TAGAAGACAC CCTTTACGAG 
GGCCTGCCGG CAGAGGTGCG GGAGAAACTG GAGGAGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LRRC8E cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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