APPL1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	APPL1
Entrez Gene ID	484716
Full Name	adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1
General protein information	Preferred Names adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 Names DCC-interacting protein 13-alpha
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_843982.4	XP_849075.1	DCC-interacting protein 13-alpha isoform X1
XM_843982.5	XP_849075.1	DCC-interacting protein 13-alpha isoform X1
XM_005632403.2	XP_005632460.1	DCC-interacting protein 13-alpha isoform X2
XM_022406558.1	XP_022262266.1	DCC-interacting protein 13-alpha isoform X4
XM_022406556.1	XP_022262264.1	DCC-interacting protein 13-alpha isoform X1
XM_005632403.3	XP_005632460.1	DCC-interacting protein 13-alpha isoform X2
XM_022406559.1	XP_022262267.1	DCC-interacting protein 13-alpha isoform X5
XM_022406557.1	XP_022262265.1	DCC-interacting protein 13-alpha isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	APPL1	XP_001173707.1
Canis lupus familiaris (dog)	APPL1	XP_849075.1
Gallus gallus (chicken)	APPL1	XP_414344.4
Bos taurus (cattle)	APPL1	NP_001092473.1
Mus musculus (house mouse)	Appl1	NP_660256.1
Homo sapiens (human)	APPL1	NP_036228.1
Rattus norvegicus (Norway rat)	Appl1	XP_006252792.1
Danio rerio (zebrafish)	appl1	NP_001121546.1
Xenopus tropicalis (tropical clawed frog)	appl1	XP_004914231.1

Gene Ontology

The following APPL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the APPL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe21520	XM_843982.5 Latest version!	Canis lupus familiaris adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe21520	XM_022406556.1 Latest version!	Canis lupus familiaris adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe21521	XM_005632403.3 Latest version!	Canis lupus familiaris adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe21522	XM_022406559.1 Latest version!	Canis lupus familiaris adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OCe295355	XM_022406557.1 Latest version!	Canis lupus familiaris adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OCe295356	XM_022406558.1 Latest version!	Canis lupus familiaris adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
OCe21520	XM_843982.4	Canis lupus familiaris adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe21521	XM_005632403.2	Canis lupus familiaris adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

APPL1 ( XM_843982.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_849075.1 Canis lupus familiaris adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe21520
Clone ID Related Accession (Same CDS sequence)	XM_843982.4 , XM_843982.5 , XM_022406556.1
Accession Version	XM_843982.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	DCC-interacting protein 13-alpha isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726096.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_843982.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGAA TCGACAAGCT GCCCATCGAG GAGACGCTGG AGGACAGCCC GCAGACAAGG 
TCTTTACTGG GTGTATTTGA AGAAGATGCT ACAGCTATTT CCAACTATAT GAACCAGTTG 
TATCAAGCTA TGCGTCGGAT TTATGATGCA CAGAATGAAT TAAGTGCAGC AACACATCTG 
ACTTCAAAAC TTTTAAAAGA ATATGAAAAA CAGCGGTTTC CATTGGGGGG TGATGATGAA 
GTTATGAGCT CTACTTTACA GCAGTTTTCA AAAGTTATAG ATGAGCTTAG CTCTTGTCAT 
GCAGTACTTT CAACGCAGCT TGCTGATGCC ATGATGTTCC CCATTACCCA ATTTAAAGAA 
AGAGATCTGA AAGAAATACT GACATTAAAG GAAGTGTTTC AGATTGCTAG TAATGACCAT 
GATGCTGCAA TTAACAGATA TAGCCGGTTA TCAAAAAAAA GAGAAAATGA CAAGGTAAAG 
TATGAAGTAA CAGAAGATGT CTACACTTCC AGAAAAAAAC AACACCAGAC CATGATGCAT 
TATTTTTGTG CATTAAACAC TCTTCAATAC AAAAAGAAAA TAGCATTGTT AGAACCTCTA 
CTTGGGTACA TGCAAGCTCA GATAAGTTTC TTTAAGATGG GTTCTGAAAA TCTTAATGAA 
CAGCTGGAAG AATTCTTGAC CAATATTGGA ACAAGTGTAC AGAATGTTCG CAGGGAAATG 
GACAGTGATG TAGAGACCAT GCAGCAGACA ATAGAAGATT TGGAAGTAGC CAGTGACCCC 
TTATATGTGC CTGACCCAGA TCCCACAAAA TTTCCTGTTA ATCGAAACTT AACCCGGAAG 
GCTGGATACC TTAATGCTAG GAATAAAACA GGTTTGGTGT CATCTACCTG GGACAGACAA 
TTTTACTTCA CGCAGGGTGG AAACTTAATG AGTCAGGCAC GTGGGGATGT AGCAGGCGGC 
CTGGCCATGG ACATTGACAA CTGTTCAGTA ATGGCTGTGG ACTGTGAAGA CAGGCGGTAC 
TGTTTTCAGA TCACTTCCTT TGATGGAAAA AAGTCTTCAA TTTTACAAGC AGAAAGTAAA 
AAAGACCATG AGGAGTGGAT CTGTACAATA AACAACATAT CCAAACAAAT ATATTTAAGT 
GAAAACCCAG AGGAAATTGC TGCACGAGTA AATCAGTCAG CTTTGGAAGC GGTCACTCCC 
TCTCCATCTT TCCAGCAGAG GCACGAGAGT TTGCGGCCAG CAGCACAATC TCGGCCACCA 
ACAGCTCGAA CCAGCAGCTC AGGATCCTTA GGATCTGAGT CCACAAACTT GGCTGCCCTT 
TCTCTAGATT CTCTCGTTGC TCCAGACACT CCAATACAAT TTGACATCAT TTCTCCCGTG 
TGTGAAGATC AGCCTGGCCA GGCAAAAGCC TTTGGCCAGG GCGGCAGGCG CACAAATCCA 
TTTGGAGAAT CTGGAGGAGG TACAAAATCT GAAACTGAAG ATTCTATTCT TCATCAGTTA 
TTTATCGTGC GATTCCTTGG TTCTATGGAG GTGAAATCAG ACGACAGTCC AGATGTTGTT 
TATGAAACAA TGCGGCAAAT CTTAGCTGCC CGGGCCATCC ATAACATCTT TCGTATGACA 
GAATCACATT TATTAGTCAC TTGTGATTGT TTAAAGTTAA TTGATCCACA GACACAACTT 
ACAAGGCTCA CATTTCCATT ACCCTGTGTA GTTTTGTACG CTACACACCA GGAAAATAAG 
AGGCTTTTTG GATTTGTTCT TCGGACATCA GGAGGGAGAA GTGAAAGTAA TCTGTCATCA 
GTCTGCTATA TATTTGAATC AAATAATGAA GGGGAAAAGA TTTGTGATTC TGTTGGACTG 
GCAAAACAGA TAGCCTTGCA TGCAGAACTG GATCGTAGGG CATCAGAAAA ACAAAAAGAA 
ATGGAGAGGG TAAAAGAGAA GCAACAGAAA GAACTCAGTA AACAAAAACA AATTGAAAAG 
GACTTAGAAG AACAGAGTCG CTTGATAGCT GCTTCCAGTA GACCGAACCA AGCCAGTAGT 
GAGGGGCAGT TTGTTGTCCT TAGCAGTAGC CAGTCAGAAG AGAGTGATTT GGGAGAAGAA 
GGAAAGAAAA GAGAGTCAGA AGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_849075.1
CDS	235..2361
Translation	MPGIDKLPIEETLEDSPQTRSLLGVFEEDATAISNYMNQLYQAMRRIYDAQNELSAATHLTSKLLKEYEKQRFPLGGDDEVMSSTLQQFSKVIDELSSCHAVLSTQLADAMMFPITQFKERDLKEILTLKEVFQIASNDHDAAINRYSRLSKKRENDKVKYEVTEDVYTSRKKQHQTMMHYFCALNTLQYKKKIALLEPLLGYMQAQISFFKMGSENLNEQLEEFLTNIGTSVQNVRREMDSDVETMQQTIEDLEVASDPLYVPDPDPTKFPVNRNLTRKAGYLNARNKTGLVSSTWDRQFYFTQGGNLMSQARGDVAGGLAMDIDNCSVMAVDCEDRRYCFQITSFDGKKSSILQAESKKDHEEWICTINNISKQIYLSENPEEIAARVNQSALEAVTPSPSFQQRHESLRPAAQSRPPTARTSSSGSLGSESTNLAALSLDSLVAPDTPIQFDIISPVCEDQPGQAKAFGQGGRRTNPFGESGGGTKSETEDSILHQLFIVRFLGSMEVKSDDSPDVVYETMRQILAARAIHNIFRMTESHLLVTCDCLKLIDPQTQLTRLTFPLPCVVLYATHQENKRLFGFVLRTSGGRSESNLSSVCYIFESNNEGEKICDSVGLAKQIALHAELDRRASEKQKEMERVKEKQQKELSKQKQIEKDLEEQSRLIAASSRPNQASSEGQFVVLSSSQSEESDLGEEGKKRESEA

Target ORF information:

RefSeq Version	XM_843982.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_843982.4

ORF Insert Sequence:

ATGCCGGGAA TCGACAAGCT GCCCATCGAG GAGACGCTGG AGGACAGCCC GCAGACAAGG 
TCTTTACTGG GTGTATTTGA AGAAGATGCT ACAGCTATTT CCAACTATAT GAACCAGTTG 
TATCAAGCTA TGCGTCGGAT TTATGATGCA CAGAATGAAT TAAGTGCAGC AACACATCTG 
ACTTCAAAAC TTTTAAAAGA ATATGAAAAA CAGCGGTTTC CATTGGGGGG TGATGATGAA 
GTTATGAGCT CTACTTTACA GCAGTTTTCA AAAGTTATAG ATGAGCTTAG CTCTTGTCAT 
GCAGTACTTT CAACGCAGCT TGCTGATGCC ATGATGTTCC CCATTACCCA ATTTAAAGAA 
AGAGATCTGA AAGAAATACT GACATTAAAG GAAGTGTTTC AGATTGCTAG TAATGACCAT 
GATGCTGCAA TTAACAGATA TAGCCGGTTA TCAAAAAAAA GAGAAAATGA CAAGGTAAAG 
TATGAAGTAA CAGAAGATGT CTACACTTCC AGAAAAAAAC AACACCAGAC CATGATGCAT 
TATTTTTGTG CATTAAACAC TCTTCAATAC AAAAAGAAAA TAGCATTGTT AGAACCTCTA 
CTTGGGTACA TGCAAGCTCA GATAAGTTTC TTTAAGATGG GTTCTGAAAA TCTTAATGAA 
CAGCTGGAAG AATTCTTGAC CAATATTGGA ACAAGTGTAC AGAATGTTCG CAGGGAAATG 
GACAGTGATG TAGAGACCAT GCAGCAGACA ATAGAAGATT TGGAAGTAGC CAGTGACCCC 
TTATATGTGC CTGACCCAGA TCCCACAAAA TTTCCTGTTA ATCGAAACTT AACCCGGAAG 
GCTGGATACC TTAATGCTAG GAATAAAACA GGTTTGGTGT CATCTACCTG GGACAGACAA 
TTTTACTTCA CGCAGGGTGG AAACTTAATG AGTCAGGCAC GTGGGGATGT AGCAGGCGGC 
CTGGCCATGG ACATTGACAA CTGTTCAGTA ATGGCTGTGG ACTGTGAAGA CAGGCGGTAC 
TGTTTTCAGA TCACTTCCTT TGATGGAAAA AAGTCTTCAA TTTTACAAGC AGAAAGTAAA 
AAAGACCATG AGGAGTGGAT CTGTACAATA AACAACATAT CCAAACAAAT ATATTTAAGT 
GAAAACCCAG AGGAAATTGC TGCACGAGTA AATCAGTCAG CTTTGGAAGC GGTCACTCCC 
TCTCCATCTT TCCAGCAGAG GCACGAGAGT TTGCGGCCAG CAGCACAATC TCGGCCACCA 
ACAGCTCGAA CCAGCAGCTC AGGATCCTTA GGATCTGAGT CCACAAACTT GGCTGCCCTT 
TCTCTAGATT CTCTCGTTGC TCCAGACACT CCAATACAAT TTGACATCAT TTCTCCCGTG 
TGTGAAGATC AGCCTGGCCA GGCAAAAGCC TTTGGCCAGG GCGGCAGGCG CACAAATCCA 
TTTGGAGAAT CTGGAGGAGG TACAAAATCT GAAACTGAAG ATTCTATTCT TCATCAGTTA 
TTTATCGTGC GATTCCTTGG TTCTATGGAG GTGAAATCAG ACGACAGTCC AGATGTTGTT 
TATGAAACAA TGCGGCAAAT CTTAGCTGCC CGGGCCATCC ATAACATCTT TCGTATGACA 
GAATCACATT TATTAGTCAC TTGTGATTGT TTAAAGTTAA TTGATCCACA GACACAACTT 
ACAAGGCTCA CATTTCCATT ACCCTGTGTA GTTTTGTACG CTACACACCA GGAAAATAAG 
AGGCTTTTTG GATTTGTTCT TCGGACATCA GGAGGGAGAA GTGAAAGTAA TCTGTCATCA 
GTCTGCTATA TATTTGAATC AAATAATGAA GGGGAAAAGA TTTGTGATTC TGTTGGACTG 
GCAAAACAGA TAGCCTTGCA TGCAGAACTG GATCGTAGGG CATCAGAAAA ACAAAAAGAA 
ATGGAGAGGG TAAAAGAGAA GCAACAGAAA GAACTCAGTA AACAAAAACA AATTGAAAAG 
GACTTAGAAG AACAGAGTCG CTTGATAGCT GCTTCCAGTA GACCGAACCA AGCCAGTAGT 
GAGGGGCAGT TTGTTGTCCT TAGCAGTAGC CAGTCAGAAG AGAGTGATTT GGGAGAAGAA 
GGAAAGAAAA GAGAGTCAGA AGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APPL1 ( XM_843982.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_849075.1 Canis lupus familiaris adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe21520
Clone ID Related Accession (Same CDS sequence)	XM_843982.4 , XM_843982.5 , XM_022406556.1
Accession Version	XM_843982.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	DCC-interacting protein 13-alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_843982.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGAA TCGACAAGCT GCCCATCGAG GAGACGCTGG AGGACAGCCC GCAGACAAGG 
TCTTTACTGG GTGTATTTGA AGAAGATGCT ACAGCTATTT CCAACTATAT GAACCAGTTG 
TATCAAGCTA TGCGTCGGAT TTATGATGCA CAGAATGAAT TAAGTGCAGC AACACATCTG 
ACTTCAAAAC TTTTAAAAGA ATATGAAAAA CAGCGGTTTC CATTGGGGGG TGATGATGAA 
GTTATGAGCT CTACTTTACA GCAGTTTTCA AAAGTTATAG ATGAGCTTAG CTCTTGTCAT 
GCAGTACTTT CAACGCAGCT TGCTGATGCC ATGATGTTCC CCATTACCCA ATTTAAAGAA 
AGAGATCTGA AAGAAATACT GACATTAAAG GAAGTGTTTC AGATTGCTAG TAATGACCAT 
GATGCTGCAA TTAACAGATA TAGCCGGTTA TCAAAAAAAA GAGAAAATGA CAAGGTAAAG 
TATGAAGTAA CAGAAGATGT CTACACTTCC AGAAAAAAAC AACACCAGAC CATGATGCAT 
TATTTTTGTG CATTAAACAC TCTTCAATAC AAAAAGAAAA TAGCATTGTT AGAACCTCTA 
CTTGGGTACA TGCAAGCTCA GATAAGTTTC TTTAAGATGG GTTCTGAAAA TCTTAATGAA 
CAGCTGGAAG AATTCTTGAC CAATATTGGA ACAAGTGTAC AGAATGTTCG CAGGGAAATG 
GACAGTGATG TAGAGACCAT GCAGCAGACA ATAGAAGATT TGGAAGTAGC CAGTGACCCC 
TTATATGTGC CTGACCCAGA TCCCACAAAA TTTCCTGTTA ATCGAAACTT AACCCGGAAG 
GCTGGATACC TTAATGCTAG GAATAAAACA GGTTTGGTGT CATCTACCTG GGACAGACAA 
TTTTACTTCA CGCAGGGTGG AAACTTAATG AGTCAGGCAC GTGGGGATGT AGCAGGCGGC 
CTGGCCATGG ACATTGACAA CTGTTCAGTA ATGGCTGTGG ACTGTGAAGA CAGGCGGTAC 
TGTTTTCAGA TCACTTCCTT TGATGGAAAA AAGTCTTCAA TTTTACAAGC AGAAAGTAAA 
AAAGACCATG AGGAGTGGAT CTGTACAATA AACAACATAT CCAAACAAAT ATATTTAAGT 
GAAAACCCAG AGGAAATTGC TGCACGAGTA AATCAGTCAG CTTTGGAAGC GGTCACTCCC 
TCTCCATCTT TCCAGCAGAG GCACGAGAGT TTGCGGCCAG CAGCACAATC TCGGCCACCA 
ACAGCTCGAA CCAGCAGCTC AGGATCCTTA GGATCTGAGT CCACAAACTT GGCTGCCCTT 
TCTCTAGATT CTCTCGTTGC TCCAGACACT CCAATACAAT TTGACATCAT TTCTCCCGTG 
TGTGAAGATC AGCCTGGCCA GGCAAAAGCC TTTGGCCAGG GCGGCAGGCG CACAAATCCA 
TTTGGAGAAT CTGGAGGAGG TACAAAATCT GAAACTGAAG ATTCTATTCT TCATCAGTTA 
TTTATCGTGC GATTCCTTGG TTCTATGGAG GTGAAATCAG ACGACAGTCC AGATGTTGTT 
TATGAAACAA TGCGGCAAAT CTTAGCTGCC CGGGCCATCC ATAACATCTT TCGTATGACA 
GAATCACATT TATTAGTCAC TTGTGATTGT TTAAAGTTAA TTGATCCACA GACACAACTT 
ACAAGGCTCA CATTTCCATT ACCCTGTGTA GTTTTGTACG CTACACACCA GGAAAATAAG 
AGGCTTTTTG GATTTGTTCT TCGGACATCA GGAGGGAGAA GTGAAAGTAA TCTGTCATCA 
GTCTGCTATA TATTTGAATC AAATAATGAA GGGGAAAAGA TTTGTGATTC TGTTGGACTG 
GCAAAACAGA TAGCCTTGCA TGCAGAACTG GATCGTAGGG CATCAGAAAA ACAAAAAGAA 
ATGGAGAGGG TAAAAGAGAA GCAACAGAAA GAACTCAGTA AACAAAAACA AATTGAAAAG 
GACTTAGAAG AACAGAGTCG CTTGATAGCT GCTTCCAGTA GACCGAACCA AGCCAGTAGT 
GAGGGGCAGT TTGTTGTCCT TAGCAGTAGC CAGTCAGAAG AGAGTGATTT GGGAGAAGAA 
GGAAAGAAAA GAGAGTCAGA AGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_849075.1
CDS	121..2247
Translation	MPGIDKLPIEETLEDSPQTRSLLGVFEEDATAISNYMNQLYQAMRRIYDAQNELSAATHLTSKLLKEYEKQRFPLGGDDEVMSSTLQQFSKVIDELSSCHAVLSTQLADAMMFPITQFKERDLKEILTLKEVFQIASNDHDAAINRYSRLSKKRENDKVKYEVTEDVYTSRKKQHQTMMHYFCALNTLQYKKKIALLEPLLGYMQAQISFFKMGSENLNEQLEEFLTNIGTSVQNVRREMDSDVETMQQTIEDLEVASDPLYVPDPDPTKFPVNRNLTRKAGYLNARNKTGLVSSTWDRQFYFTQGGNLMSQARGDVAGGLAMDIDNCSVMAVDCEDRRYCFQITSFDGKKSSILQAESKKDHEEWICTINNISKQIYLSENPEEIAARVNQSALEAVTPSPSFQQRHESLRPAAQSRPPTARTSSSGSLGSESTNLAALSLDSLVAPDTPIQFDIISPVCEDQPGQAKAFGQGGRRTNPFGESGGGTKSETEDSILHQLFIVRFLGSMEVKSDDSPDVVYETMRQILAARAIHNIFRMTESHLLVTCDCLKLIDPQTQLTRLTFPLPCVVLYATHQENKRLFGFVLRTSGGRSESNLSSVCYIFESNNEGEKICDSVGLAKQIALHAELDRRASEKQKEMERVKEKQQKELSKQKQIEKDLEEQSRLIAASSRPNQASSEGQFVVLSSSQSEESDLGEEGKKRESEA

Target ORF information:

RefSeq Version	XM_843982.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_843982.5

ORF Insert Sequence:

ATGCCGGGAA TCGACAAGCT GCCCATCGAG GAGACGCTGG AGGACAGCCC GCAGACAAGG 
TCTTTACTGG GTGTATTTGA AGAAGATGCT ACAGCTATTT CCAACTATAT GAACCAGTTG 
TATCAAGCTA TGCGTCGGAT TTATGATGCA CAGAATGAAT TAAGTGCAGC AACACATCTG 
ACTTCAAAAC TTTTAAAAGA ATATGAAAAA CAGCGGTTTC CATTGGGGGG TGATGATGAA 
GTTATGAGCT CTACTTTACA GCAGTTTTCA AAAGTTATAG ATGAGCTTAG CTCTTGTCAT 
GCAGTACTTT CAACGCAGCT TGCTGATGCC ATGATGTTCC CCATTACCCA ATTTAAAGAA 
AGAGATCTGA AAGAAATACT GACATTAAAG GAAGTGTTTC AGATTGCTAG TAATGACCAT 
GATGCTGCAA TTAACAGATA TAGCCGGTTA TCAAAAAAAA GAGAAAATGA CAAGGTAAAG 
TATGAAGTAA CAGAAGATGT CTACACTTCC AGAAAAAAAC AACACCAGAC CATGATGCAT 
TATTTTTGTG CATTAAACAC TCTTCAATAC AAAAAGAAAA TAGCATTGTT AGAACCTCTA 
CTTGGGTACA TGCAAGCTCA GATAAGTTTC TTTAAGATGG GTTCTGAAAA TCTTAATGAA 
CAGCTGGAAG AATTCTTGAC CAATATTGGA ACAAGTGTAC AGAATGTTCG CAGGGAAATG 
GACAGTGATG TAGAGACCAT GCAGCAGACA ATAGAAGATT TGGAAGTAGC CAGTGACCCC 
TTATATGTGC CTGACCCAGA TCCCACAAAA TTTCCTGTTA ATCGAAACTT AACCCGGAAG 
GCTGGATACC TTAATGCTAG GAATAAAACA GGTTTGGTGT CATCTACCTG GGACAGACAA 
TTTTACTTCA CGCAGGGTGG AAACTTAATG AGTCAGGCAC GTGGGGATGT AGCAGGCGGC 
CTGGCCATGG ACATTGACAA CTGTTCAGTA ATGGCTGTGG ACTGTGAAGA CAGGCGGTAC 
TGTTTTCAGA TCACTTCCTT TGATGGAAAA AAGTCTTCAA TTTTACAAGC AGAAAGTAAA 
AAAGACCATG AGGAGTGGAT CTGTACAATA AACAACATAT CCAAACAAAT ATATTTAAGT 
GAAAACCCAG AGGAAATTGC TGCACGAGTA AATCAGTCAG CTTTGGAAGC GGTCACTCCC 
TCTCCATCTT TCCAGCAGAG GCACGAGAGT TTGCGGCCAG CAGCACAATC TCGGCCACCA 
ACAGCTCGAA CCAGCAGCTC AGGATCCTTA GGATCTGAGT CCACAAACTT GGCTGCCCTT 
TCTCTAGATT CTCTCGTTGC TCCAGACACT CCAATACAAT TTGACATCAT TTCTCCCGTG 
TGTGAAGATC AGCCTGGCCA GGCAAAAGCC TTTGGCCAGG GCGGCAGGCG CACAAATCCA 
TTTGGAGAAT CTGGAGGAGG TACAAAATCT GAAACTGAAG ATTCTATTCT TCATCAGTTA 
TTTATCGTGC GATTCCTTGG TTCTATGGAG GTGAAATCAG ACGACAGTCC AGATGTTGTT 
TATGAAACAA TGCGGCAAAT CTTAGCTGCC CGGGCCATCC ATAACATCTT TCGTATGACA 
GAATCACATT TATTAGTCAC TTGTGATTGT TTAAAGTTAA TTGATCCACA GACACAACTT 
ACAAGGCTCA CATTTCCATT ACCCTGTGTA GTTTTGTACG CTACACACCA GGAAAATAAG 
AGGCTTTTTG GATTTGTTCT TCGGACATCA GGAGGGAGAA GTGAAAGTAA TCTGTCATCA 
GTCTGCTATA TATTTGAATC AAATAATGAA GGGGAAAAGA TTTGTGATTC TGTTGGACTG 
GCAAAACAGA TAGCCTTGCA TGCAGAACTG GATCGTAGGG CATCAGAAAA ACAAAAAGAA 
ATGGAGAGGG TAAAAGAGAA GCAACAGAAA GAACTCAGTA AACAAAAACA AATTGAAAAG 
GACTTAGAAG AACAGAGTCG CTTGATAGCT GCTTCCAGTA GACCGAACCA AGCCAGTAGT 
GAGGGGCAGT TTGTTGTCCT TAGCAGTAGC CAGTCAGAAG AGAGTGATTT GGGAGAAGAA 
GGAAAGAAAA GAGAGTCAGA AGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APPL1 ( XM_022406556.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022262264.1 Canis lupus familiaris adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe21520
Clone ID Related Accession (Same CDS sequence)	XM_843982.4 , XM_843982.5 , XM_022406556.1
Accession Version	XM_022406556.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	DCC-interacting protein 13-alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGAA TCGACAAGCT GCCCATCGAG GAGACGCTGG AGGACAGCCC GCAGACAAGG 
TCTTTACTGG GTGTATTTGA AGAAGATGCT ACAGCTATTT CCAACTATAT GAACCAGTTG 
TATCAAGCTA TGCGTCGGAT TTATGATGCA CAGAATGAAT TAAGTGCAGC AACACATCTG 
ACTTCAAAAC TTTTAAAAGA ATATGAAAAA CAGCGGTTTC CATTGGGGGG TGATGATGAA 
GTTATGAGCT CTACTTTACA GCAGTTTTCA AAAGTTATAG ATGAGCTTAG CTCTTGTCAT 
GCAGTACTTT CAACGCAGCT TGCTGATGCC ATGATGTTCC CCATTACCCA ATTTAAAGAA 
AGAGATCTGA AAGAAATACT GACATTAAAG GAAGTGTTTC AGATTGCTAG TAATGACCAT 
GATGCTGCAA TTAACAGATA TAGCCGGTTA TCAAAAAAAA GAGAAAATGA CAAGGTAAAG 
TATGAAGTAA CAGAAGATGT CTACACTTCC AGAAAAAAAC AACACCAGAC CATGATGCAT 
TATTTTTGTG CATTAAACAC TCTTCAATAC AAAAAGAAAA TAGCATTGTT AGAACCTCTA 
CTTGGGTACA TGCAAGCTCA GATAAGTTTC TTTAAGATGG GTTCTGAAAA TCTTAATGAA 
CAGCTGGAAG AATTCTTGAC CAATATTGGA ACAAGTGTAC AGAATGTTCG CAGGGAAATG 
GACAGTGATG TAGAGACCAT GCAGCAGACA ATAGAAGATT TGGAAGTAGC CAGTGACCCC 
TTATATGTGC CTGACCCAGA TCCCACAAAA TTTCCTGTTA ATCGAAACTT AACCCGGAAG 
GCTGGATACC TTAATGCTAG GAATAAAACA GGTTTGGTGT CATCTACCTG GGACAGACAA 
TTTTACTTCA CGCAGGGTGG AAACTTAATG AGTCAGGCAC GTGGGGATGT AGCAGGCGGC 
CTGGCCATGG ACATTGACAA CTGTTCAGTA ATGGCTGTGG ACTGTGAAGA CAGGCGGTAC 
TGTTTTCAGA TCACTTCCTT TGATGGAAAA AAGTCTTCAA TTTTACAAGC AGAAAGTAAA 
AAAGACCATG AGGAGTGGAT CTGTACAATA AACAACATAT CCAAACAAAT ATATTTAAGT 
GAAAACCCAG AGGAAATTGC TGCACGAGTA AATCAGTCAG CTTTGGAAGC GGTCACTCCC 
TCTCCATCTT TCCAGCAGAG GCACGAGAGT TTGCGGCCAG CAGCACAATC TCGGCCACCA 
ACAGCTCGAA CCAGCAGCTC AGGATCCTTA GGATCTGAGT CCACAAACTT GGCTGCCCTT 
TCTCTAGATT CTCTCGTTGC TCCAGACACT CCAATACAAT TTGACATCAT TTCTCCCGTG 
TGTGAAGATC AGCCTGGCCA GGCAAAAGCC TTTGGCCAGG GCGGCAGGCG CACAAATCCA 
TTTGGAGAAT CTGGAGGAGG TACAAAATCT GAAACTGAAG ATTCTATTCT TCATCAGTTA 
TTTATCGTGC GATTCCTTGG TTCTATGGAG GTGAAATCAG ACGACAGTCC AGATGTTGTT 
TATGAAACAA TGCGGCAAAT CTTAGCTGCC CGGGCCATCC ATAACATCTT TCGTATGACA 
GAATCACATT TATTAGTCAC TTGTGATTGT TTAAAGTTAA TTGATCCACA GACACAACTT 
ACAAGGCTCA CATTTCCATT ACCCTGTGTA GTTTTGTACG CTACACACCA GGAAAATAAG 
AGGCTTTTTG GATTTGTTCT TCGGACATCA GGAGGGAGAA GTGAAAGTAA TCTGTCATCA 
GTCTGCTATA TATTTGAATC AAATAATGAA GGGGAAAAGA TTTGTGATTC TGTTGGACTG 
GCAAAACAGA TAGCCTTGCA TGCAGAACTG GATCGTAGGG CATCAGAAAA ACAAAAAGAA 
ATGGAGAGGG TAAAAGAGAA GCAACAGAAA GAACTCAGTA AACAAAAACA AATTGAAAAG 
GACTTAGAAG AACAGAGTCG CTTGATAGCT GCTTCCAGTA GACCGAACCA AGCCAGTAGT 
GAGGGGCAGT TTGTTGTCCT TAGCAGTAGC CAGTCAGAAG AGAGTGATTT GGGAGAAGAA 
GGAAAGAAAA GAGAGTCAGA AGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022262264.1
CDS	122..2248
Translation	MPGIDKLPIEETLEDSPQTRSLLGVFEEDATAISNYMNQLYQAMRRIYDAQNELSAATHLTSKLLKEYEKQRFPLGGDDEVMSSTLQQFSKVIDELSSCHAVLSTQLADAMMFPITQFKERDLKEILTLKEVFQIASNDHDAAINRYSRLSKKRENDKVKYEVTEDVYTSRKKQHQTMMHYFCALNTLQYKKKIALLEPLLGYMQAQISFFKMGSENLNEQLEEFLTNIGTSVQNVRREMDSDVETMQQTIEDLEVASDPLYVPDPDPTKFPVNRNLTRKAGYLNARNKTGLVSSTWDRQFYFTQGGNLMSQARGDVAGGLAMDIDNCSVMAVDCEDRRYCFQITSFDGKKSSILQAESKKDHEEWICTINNISKQIYLSENPEEIAARVNQSALEAVTPSPSFQQRHESLRPAAQSRPPTARTSSSGSLGSESTNLAALSLDSLVAPDTPIQFDIISPVCEDQPGQAKAFGQGGRRTNPFGESGGGTKSETEDSILHQLFIVRFLGSMEVKSDDSPDVVYETMRQILAARAIHNIFRMTESHLLVTCDCLKLIDPQTQLTRLTFPLPCVVLYATHQENKRLFGFVLRTSGGRSESNLSSVCYIFESNNEGEKICDSVGLAKQIALHAELDRRASEKQKEMERVKEKQQKELSKQKQIEKDLEEQSRLIAASSRPNQASSEGQFVVLSSSQSEESDLGEEGKKRESEA

Target ORF information:

RefSeq Version	XM_022406556.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022406556.1

ORF Insert Sequence:

ATGCCGGGAA TCGACAAGCT GCCCATCGAG GAGACGCTGG AGGACAGCCC GCAGACAAGG 
TCTTTACTGG GTGTATTTGA AGAAGATGCT ACAGCTATTT CCAACTATAT GAACCAGTTG 
TATCAAGCTA TGCGTCGGAT TTATGATGCA CAGAATGAAT TAAGTGCAGC AACACATCTG 
ACTTCAAAAC TTTTAAAAGA ATATGAAAAA CAGCGGTTTC CATTGGGGGG TGATGATGAA 
GTTATGAGCT CTACTTTACA GCAGTTTTCA AAAGTTATAG ATGAGCTTAG CTCTTGTCAT 
GCAGTACTTT CAACGCAGCT TGCTGATGCC ATGATGTTCC CCATTACCCA ATTTAAAGAA 
AGAGATCTGA AAGAAATACT GACATTAAAG GAAGTGTTTC AGATTGCTAG TAATGACCAT 
GATGCTGCAA TTAACAGATA TAGCCGGTTA TCAAAAAAAA GAGAAAATGA CAAGGTAAAG 
TATGAAGTAA CAGAAGATGT CTACACTTCC AGAAAAAAAC AACACCAGAC CATGATGCAT 
TATTTTTGTG CATTAAACAC TCTTCAATAC AAAAAGAAAA TAGCATTGTT AGAACCTCTA 
CTTGGGTACA TGCAAGCTCA GATAAGTTTC TTTAAGATGG GTTCTGAAAA TCTTAATGAA 
CAGCTGGAAG AATTCTTGAC CAATATTGGA ACAAGTGTAC AGAATGTTCG CAGGGAAATG 
GACAGTGATG TAGAGACCAT GCAGCAGACA ATAGAAGATT TGGAAGTAGC CAGTGACCCC 
TTATATGTGC CTGACCCAGA TCCCACAAAA TTTCCTGTTA ATCGAAACTT AACCCGGAAG 
GCTGGATACC TTAATGCTAG GAATAAAACA GGTTTGGTGT CATCTACCTG GGACAGACAA 
TTTTACTTCA CGCAGGGTGG AAACTTAATG AGTCAGGCAC GTGGGGATGT AGCAGGCGGC 
CTGGCCATGG ACATTGACAA CTGTTCAGTA ATGGCTGTGG ACTGTGAAGA CAGGCGGTAC 
TGTTTTCAGA TCACTTCCTT TGATGGAAAA AAGTCTTCAA TTTTACAAGC AGAAAGTAAA 
AAAGACCATG AGGAGTGGAT CTGTACAATA AACAACATAT CCAAACAAAT ATATTTAAGT 
GAAAACCCAG AGGAAATTGC TGCACGAGTA AATCAGTCAG CTTTGGAAGC GGTCACTCCC 
TCTCCATCTT TCCAGCAGAG GCACGAGAGT TTGCGGCCAG CAGCACAATC TCGGCCACCA 
ACAGCTCGAA CCAGCAGCTC AGGATCCTTA GGATCTGAGT CCACAAACTT GGCTGCCCTT 
TCTCTAGATT CTCTCGTTGC TCCAGACACT CCAATACAAT TTGACATCAT TTCTCCCGTG 
TGTGAAGATC AGCCTGGCCA GGCAAAAGCC TTTGGCCAGG GCGGCAGGCG CACAAATCCA 
TTTGGAGAAT CTGGAGGAGG TACAAAATCT GAAACTGAAG ATTCTATTCT TCATCAGTTA 
TTTATCGTGC GATTCCTTGG TTCTATGGAG GTGAAATCAG ACGACAGTCC AGATGTTGTT 
TATGAAACAA TGCGGCAAAT CTTAGCTGCC CGGGCCATCC ATAACATCTT TCGTATGACA 
GAATCACATT TATTAGTCAC TTGTGATTGT TTAAAGTTAA TTGATCCACA GACACAACTT 
ACAAGGCTCA CATTTCCATT ACCCTGTGTA GTTTTGTACG CTACACACCA GGAAAATAAG 
AGGCTTTTTG GATTTGTTCT TCGGACATCA GGAGGGAGAA GTGAAAGTAA TCTGTCATCA 
GTCTGCTATA TATTTGAATC AAATAATGAA GGGGAAAAGA TTTGTGATTC TGTTGGACTG 
GCAAAACAGA TAGCCTTGCA TGCAGAACTG GATCGTAGGG CATCAGAAAA ACAAAAAGAA 
ATGGAGAGGG TAAAAGAGAA GCAACAGAAA GAACTCAGTA AACAAAAACA AATTGAAAAG 
GACTTAGAAG AACAGAGTCG CTTGATAGCT GCTTCCAGTA GACCGAACCA AGCCAGTAGT 
GAGGGGCAGT TTGTTGTCCT TAGCAGTAGC CAGTCAGAAG AGAGTGATTT GGGAGAAGAA 
GGAAAGAAAA GAGAGTCAGA AGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APPL1 ( XM_005632403.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005632460.1 Canis lupus familiaris adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe21521
Clone ID Related Accession (Same CDS sequence)	XM_005632403.2 , XM_005632403.3
Accession Version	XM_005632403.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	DCC-interacting protein 13-alpha isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726096.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005632403.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGAA TCGACAAGCT GCCCATCGAG GAGACGCTGG AGGACAGCCC GCAGACAAGG 
TCTTTACTGG GTGTATTTGA AGAAGATGCT ACAGCTATTT CCAACTATAT GAACCAGTTG 
TATCAAGCTA TGCGTCGGAT TTATGATGCA CAGCGGTTTC CATTGGGGGG TGATGATGAA 
GTTATGAGCT CTACTTTACA GCAGTTTTCA AAAGTTATAG ATGAGCTTAG CTCTTGTCAT 
GCAGTACTTT CAACGCAGCT TGCTGATGCC ATGATGTTCC CCATTACCCA ATTTAAAGAA 
AGAGATCTGA AAGAAATACT GACATTAAAG GAAGTGTTTC AGATTGCTAG TAATGACCAT 
GATGCTGCAA TTAACAGATA TAGCCGGTTA TCAAAAAAAA GAGAAAATGA CAAGGTAAAG 
TATGAAGTAA CAGAAGATGT CTACACTTCC AGAAAAAAAC AACACCAGAC CATGATGCAT 
TATTTTTGTG CATTAAACAC TCTTCAATAC AAAAAGAAAA TAGCATTGTT AGAACCTCTA 
CTTGGGTACA TGCAAGCTCA GATAAGTTTC TTTAAGATGG GTTCTGAAAA TCTTAATGAA 
CAGCTGGAAG AATTCTTGAC CAATATTGGA ACAAGTGTAC AGAATGTTCG CAGGGAAATG 
GACAGTGATG TAGAGACCAT GCAGCAGACA ATAGAAGATT TGGAAGTAGC CAGTGACCCC 
TTATATGTGC CTGACCCAGA TCCCACAAAA TTTCCTGTTA ATCGAAACTT AACCCGGAAG 
GCTGGATACC TTAATGCTAG GAATAAAACA GGTTTGGTGT CATCTACCTG GGACAGACAA 
TTTTACTTCA CGCAGGGTGG AAACTTAATG AGTCAGGCAC GTGGGGATGT AGCAGGCGGC 
CTGGCCATGG ACATTGACAA CTGTTCAGTA ATGGCTGTGG ACTGTGAAGA CAGGCGGTAC 
TGTTTTCAGA TCACTTCCTT TGATGGAAAA AAGTCTTCAA TTTTACAAGC AGAAAGTAAA 
AAAGACCATG AGGAGTGGAT CTGTACAATA AACAACATAT CCAAACAAAT ATATTTAAGT 
GAAAACCCAG AGGAAATTGC TGCACGAGTA AATCAGTCAG CTTTGGAAGC GGTCACTCCC 
TCTCCATCTT TCCAGCAGAG GCACGAGAGT TTGCGGCCAG CAGCACAATC TCGGCCACCA 
ACAGCTCGAA CCAGCAGCTC AGGATCCTTA GGATCTGAGT CCACAAACTT GGCTGCCCTT 
TCTCTAGATT CTCTCGTTGC TCCAGACACT CCAATACAAT TTGACATCAT TTCTCCCGTG 
TGTGAAGATC AGCCTGGCCA GGCAAAAGCC TTTGGCCAGG GCGGCAGGCG CACAAATCCA 
TTTGGAGAAT CTGGAGGAGG TACAAAATCT GAAACTGAAG ATTCTATTCT TCATCAGTTA 
TTTATCGTGC GATTCCTTGG TTCTATGGAG GTGAAATCAG ACGACAGTCC AGATGTTGTT 
TATGAAACAA TGCGGCAAAT CTTAGCTGCC CGGGCCATCC ATAACATCTT TCGTATGACA 
GAATCACATT TATTAGTCAC TTGTGATTGT TTAAAGTTAA TTGATCCACA GACACAACTT 
ACAAGGCTCA CATTTCCATT ACCCTGTGTA GTTTTGTACG CTACACACCA GGAAAATAAG 
AGGCTTTTTG GATTTGTTCT TCGGACATCA GGAGGGAGAA GTGAAAGTAA TCTGTCATCA 
GTCTGCTATA TATTTGAATC AAATAATGAA GGGGAAAAGA TTTGTGATTC TGTTGGACTG 
GCAAAACAGA TAGCCTTGCA TGCAGAACTG GATCGTAGGG CATCAGAAAA ACAAAAAGAA 
ATGGAGAGGG TAAAAGAGAA GCAACAGAAA GAACTCAGTA AACAAAAACA AATTGAAAAG 
GACTTAGAAG AACAGAGTCG CTTGATAGCT GCTTCCAGTA GACCGAACCA AGCCAGTAGT 
GAGGGGCAGT TTGTTGTCCT TAGCAGTAGC CAGTCAGAAG AGAGTGATTT GGGAGAAGAA 
GGAAAGAAAA GAGAGTCAGA AGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005632460.1
CDS	235..2301
Translation	MPGIDKLPIEETLEDSPQTRSLLGVFEEDATAISNYMNQLYQAMRRIYDAQRFPLGGDDEVMSSTLQQFSKVIDELSSCHAVLSTQLADAMMFPITQFKERDLKEILTLKEVFQIASNDHDAAINRYSRLSKKRENDKVKYEVTEDVYTSRKKQHQTMMHYFCALNTLQYKKKIALLEPLLGYMQAQISFFKMGSENLNEQLEEFLTNIGTSVQNVRREMDSDVETMQQTIEDLEVASDPLYVPDPDPTKFPVNRNLTRKAGYLNARNKTGLVSSTWDRQFYFTQGGNLMSQARGDVAGGLAMDIDNCSVMAVDCEDRRYCFQITSFDGKKSSILQAESKKDHEEWICTINNISKQIYLSENPEEIAARVNQSALEAVTPSPSFQQRHESLRPAAQSRPPTARTSSSGSLGSESTNLAALSLDSLVAPDTPIQFDIISPVCEDQPGQAKAFGQGGRRTNPFGESGGGTKSETEDSILHQLFIVRFLGSMEVKSDDSPDVVYETMRQILAARAIHNIFRMTESHLLVTCDCLKLIDPQTQLTRLTFPLPCVVLYATHQENKRLFGFVLRTSGGRSESNLSSVCYIFESNNEGEKICDSVGLAKQIALHAELDRRASEKQKEMERVKEKQQKELSKQKQIEKDLEEQSRLIAASSRPNQASSEGQFVVLSSSQSEESDLGEEGKKRESEA

Target ORF information:

RefSeq Version	XM_005632403.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005632403.2

ORF Insert Sequence:

ATGCCGGGAA TCGACAAGCT GCCCATCGAG GAGACGCTGG AGGACAGCCC GCAGACAAGG 
TCTTTACTGG GTGTATTTGA AGAAGATGCT ACAGCTATTT CCAACTATAT GAACCAGTTG 
TATCAAGCTA TGCGTCGGAT TTATGATGCA CAGCGGTTTC CATTGGGGGG TGATGATGAA 
GTTATGAGCT CTACTTTACA GCAGTTTTCA AAAGTTATAG ATGAGCTTAG CTCTTGTCAT 
GCAGTACTTT CAACGCAGCT TGCTGATGCC ATGATGTTCC CCATTACCCA ATTTAAAGAA 
AGAGATCTGA AAGAAATACT GACATTAAAG GAAGTGTTTC AGATTGCTAG TAATGACCAT 
GATGCTGCAA TTAACAGATA TAGCCGGTTA TCAAAAAAAA GAGAAAATGA CAAGGTAAAG 
TATGAAGTAA CAGAAGATGT CTACACTTCC AGAAAAAAAC AACACCAGAC CATGATGCAT 
TATTTTTGTG CATTAAACAC TCTTCAATAC AAAAAGAAAA TAGCATTGTT AGAACCTCTA 
CTTGGGTACA TGCAAGCTCA GATAAGTTTC TTTAAGATGG GTTCTGAAAA TCTTAATGAA 
CAGCTGGAAG AATTCTTGAC CAATATTGGA ACAAGTGTAC AGAATGTTCG CAGGGAAATG 
GACAGTGATG TAGAGACCAT GCAGCAGACA ATAGAAGATT TGGAAGTAGC CAGTGACCCC 
TTATATGTGC CTGACCCAGA TCCCACAAAA TTTCCTGTTA ATCGAAACTT AACCCGGAAG 
GCTGGATACC TTAATGCTAG GAATAAAACA GGTTTGGTGT CATCTACCTG GGACAGACAA 
TTTTACTTCA CGCAGGGTGG AAACTTAATG AGTCAGGCAC GTGGGGATGT AGCAGGCGGC 
CTGGCCATGG ACATTGACAA CTGTTCAGTA ATGGCTGTGG ACTGTGAAGA CAGGCGGTAC 
TGTTTTCAGA TCACTTCCTT TGATGGAAAA AAGTCTTCAA TTTTACAAGC AGAAAGTAAA 
AAAGACCATG AGGAGTGGAT CTGTACAATA AACAACATAT CCAAACAAAT ATATTTAAGT 
GAAAACCCAG AGGAAATTGC TGCACGAGTA AATCAGTCAG CTTTGGAAGC GGTCACTCCC 
TCTCCATCTT TCCAGCAGAG GCACGAGAGT TTGCGGCCAG CAGCACAATC TCGGCCACCA 
ACAGCTCGAA CCAGCAGCTC AGGATCCTTA GGATCTGAGT CCACAAACTT GGCTGCCCTT 
TCTCTAGATT CTCTCGTTGC TCCAGACACT CCAATACAAT TTGACATCAT TTCTCCCGTG 
TGTGAAGATC AGCCTGGCCA GGCAAAAGCC TTTGGCCAGG GCGGCAGGCG CACAAATCCA 
TTTGGAGAAT CTGGAGGAGG TACAAAATCT GAAACTGAAG ATTCTATTCT TCATCAGTTA 
TTTATCGTGC GATTCCTTGG TTCTATGGAG GTGAAATCAG ACGACAGTCC AGATGTTGTT 
TATGAAACAA TGCGGCAAAT CTTAGCTGCC CGGGCCATCC ATAACATCTT TCGTATGACA 
GAATCACATT TATTAGTCAC TTGTGATTGT TTAAAGTTAA TTGATCCACA GACACAACTT 
ACAAGGCTCA CATTTCCATT ACCCTGTGTA GTTTTGTACG CTACACACCA GGAAAATAAG 
AGGCTTTTTG GATTTGTTCT TCGGACATCA GGAGGGAGAA GTGAAAGTAA TCTGTCATCA 
GTCTGCTATA TATTTGAATC AAATAATGAA GGGGAAAAGA TTTGTGATTC TGTTGGACTG 
GCAAAACAGA TAGCCTTGCA TGCAGAACTG GATCGTAGGG CATCAGAAAA ACAAAAAGAA 
ATGGAGAGGG TAAAAGAGAA GCAACAGAAA GAACTCAGTA AACAAAAACA AATTGAAAAG 
GACTTAGAAG AACAGAGTCG CTTGATAGCT GCTTCCAGTA GACCGAACCA AGCCAGTAGT 
GAGGGGCAGT TTGTTGTCCT TAGCAGTAGC CAGTCAGAAG AGAGTGATTT GGGAGAAGAA 
GGAAAGAAAA GAGAGTCAGA AGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APPL1 ( XM_005632403.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005632460.1 Canis lupus familiaris adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe21521
Clone ID Related Accession (Same CDS sequence)	XM_005632403.2 , XM_005632403.3
Accession Version	XM_005632403.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	DCC-interacting protein 13-alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005632403.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGAA TCGACAAGCT GCCCATCGAG GAGACGCTGG AGGACAGCCC GCAGACAAGG 
TCTTTACTGG GTGTATTTGA AGAAGATGCT ACAGCTATTT CCAACTATAT GAACCAGTTG 
TATCAAGCTA TGCGTCGGAT TTATGATGCA CAGCGGTTTC CATTGGGGGG TGATGATGAA 
GTTATGAGCT CTACTTTACA GCAGTTTTCA AAAGTTATAG ATGAGCTTAG CTCTTGTCAT 
GCAGTACTTT CAACGCAGCT TGCTGATGCC ATGATGTTCC CCATTACCCA ATTTAAAGAA 
AGAGATCTGA AAGAAATACT GACATTAAAG GAAGTGTTTC AGATTGCTAG TAATGACCAT 
GATGCTGCAA TTAACAGATA TAGCCGGTTA TCAAAAAAAA GAGAAAATGA CAAGGTAAAG 
TATGAAGTAA CAGAAGATGT CTACACTTCC AGAAAAAAAC AACACCAGAC CATGATGCAT 
TATTTTTGTG CATTAAACAC TCTTCAATAC AAAAAGAAAA TAGCATTGTT AGAACCTCTA 
CTTGGGTACA TGCAAGCTCA GATAAGTTTC TTTAAGATGG GTTCTGAAAA TCTTAATGAA 
CAGCTGGAAG AATTCTTGAC CAATATTGGA ACAAGTGTAC AGAATGTTCG CAGGGAAATG 
GACAGTGATG TAGAGACCAT GCAGCAGACA ATAGAAGATT TGGAAGTAGC CAGTGACCCC 
TTATATGTGC CTGACCCAGA TCCCACAAAA TTTCCTGTTA ATCGAAACTT AACCCGGAAG 
GCTGGATACC TTAATGCTAG GAATAAAACA GGTTTGGTGT CATCTACCTG GGACAGACAA 
TTTTACTTCA CGCAGGGTGG AAACTTAATG AGTCAGGCAC GTGGGGATGT AGCAGGCGGC 
CTGGCCATGG ACATTGACAA CTGTTCAGTA ATGGCTGTGG ACTGTGAAGA CAGGCGGTAC 
TGTTTTCAGA TCACTTCCTT TGATGGAAAA AAGTCTTCAA TTTTACAAGC AGAAAGTAAA 
AAAGACCATG AGGAGTGGAT CTGTACAATA AACAACATAT CCAAACAAAT ATATTTAAGT 
GAAAACCCAG AGGAAATTGC TGCACGAGTA AATCAGTCAG CTTTGGAAGC GGTCACTCCC 
TCTCCATCTT TCCAGCAGAG GCACGAGAGT TTGCGGCCAG CAGCACAATC TCGGCCACCA 
ACAGCTCGAA CCAGCAGCTC AGGATCCTTA GGATCTGAGT CCACAAACTT GGCTGCCCTT 
TCTCTAGATT CTCTCGTTGC TCCAGACACT CCAATACAAT TTGACATCAT TTCTCCCGTG 
TGTGAAGATC AGCCTGGCCA GGCAAAAGCC TTTGGCCAGG GCGGCAGGCG CACAAATCCA 
TTTGGAGAAT CTGGAGGAGG TACAAAATCT GAAACTGAAG ATTCTATTCT TCATCAGTTA 
TTTATCGTGC GATTCCTTGG TTCTATGGAG GTGAAATCAG ACGACAGTCC AGATGTTGTT 
TATGAAACAA TGCGGCAAAT CTTAGCTGCC CGGGCCATCC ATAACATCTT TCGTATGACA 
GAATCACATT TATTAGTCAC TTGTGATTGT TTAAAGTTAA TTGATCCACA GACACAACTT 
ACAAGGCTCA CATTTCCATT ACCCTGTGTA GTTTTGTACG CTACACACCA GGAAAATAAG 
AGGCTTTTTG GATTTGTTCT TCGGACATCA GGAGGGAGAA GTGAAAGTAA TCTGTCATCA 
GTCTGCTATA TATTTGAATC AAATAATGAA GGGGAAAAGA TTTGTGATTC TGTTGGACTG 
GCAAAACAGA TAGCCTTGCA TGCAGAACTG GATCGTAGGG CATCAGAAAA ACAAAAAGAA 
ATGGAGAGGG TAAAAGAGAA GCAACAGAAA GAACTCAGTA AACAAAAACA AATTGAAAAG 
GACTTAGAAG AACAGAGTCG CTTGATAGCT GCTTCCAGTA GACCGAACCA AGCCAGTAGT 
GAGGGGCAGT TTGTTGTCCT TAGCAGTAGC CAGTCAGAAG AGAGTGATTT GGGAGAAGAA 
GGAAAGAAAA GAGAGTCAGA AGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005632460.1
CDS	121..2187
Translation	MPGIDKLPIEETLEDSPQTRSLLGVFEEDATAISNYMNQLYQAMRRIYDAQRFPLGGDDEVMSSTLQQFSKVIDELSSCHAVLSTQLADAMMFPITQFKERDLKEILTLKEVFQIASNDHDAAINRYSRLSKKRENDKVKYEVTEDVYTSRKKQHQTMMHYFCALNTLQYKKKIALLEPLLGYMQAQISFFKMGSENLNEQLEEFLTNIGTSVQNVRREMDSDVETMQQTIEDLEVASDPLYVPDPDPTKFPVNRNLTRKAGYLNARNKTGLVSSTWDRQFYFTQGGNLMSQARGDVAGGLAMDIDNCSVMAVDCEDRRYCFQITSFDGKKSSILQAESKKDHEEWICTINNISKQIYLSENPEEIAARVNQSALEAVTPSPSFQQRHESLRPAAQSRPPTARTSSSGSLGSESTNLAALSLDSLVAPDTPIQFDIISPVCEDQPGQAKAFGQGGRRTNPFGESGGGTKSETEDSILHQLFIVRFLGSMEVKSDDSPDVVYETMRQILAARAIHNIFRMTESHLLVTCDCLKLIDPQTQLTRLTFPLPCVVLYATHQENKRLFGFVLRTSGGRSESNLSSVCYIFESNNEGEKICDSVGLAKQIALHAELDRRASEKQKEMERVKEKQQKELSKQKQIEKDLEEQSRLIAASSRPNQASSEGQFVVLSSSQSEESDLGEEGKKRESEA

Target ORF information:

RefSeq Version	XM_005632403.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005632403.3

ORF Insert Sequence:

ATGCCGGGAA TCGACAAGCT GCCCATCGAG GAGACGCTGG AGGACAGCCC GCAGACAAGG 
TCTTTACTGG GTGTATTTGA AGAAGATGCT ACAGCTATTT CCAACTATAT GAACCAGTTG 
TATCAAGCTA TGCGTCGGAT TTATGATGCA CAGCGGTTTC CATTGGGGGG TGATGATGAA 
GTTATGAGCT CTACTTTACA GCAGTTTTCA AAAGTTATAG ATGAGCTTAG CTCTTGTCAT 
GCAGTACTTT CAACGCAGCT TGCTGATGCC ATGATGTTCC CCATTACCCA ATTTAAAGAA 
AGAGATCTGA AAGAAATACT GACATTAAAG GAAGTGTTTC AGATTGCTAG TAATGACCAT 
GATGCTGCAA TTAACAGATA TAGCCGGTTA TCAAAAAAAA GAGAAAATGA CAAGGTAAAG 
TATGAAGTAA CAGAAGATGT CTACACTTCC AGAAAAAAAC AACACCAGAC CATGATGCAT 
TATTTTTGTG CATTAAACAC TCTTCAATAC AAAAAGAAAA TAGCATTGTT AGAACCTCTA 
CTTGGGTACA TGCAAGCTCA GATAAGTTTC TTTAAGATGG GTTCTGAAAA TCTTAATGAA 
CAGCTGGAAG AATTCTTGAC CAATATTGGA ACAAGTGTAC AGAATGTTCG CAGGGAAATG 
GACAGTGATG TAGAGACCAT GCAGCAGACA ATAGAAGATT TGGAAGTAGC CAGTGACCCC 
TTATATGTGC CTGACCCAGA TCCCACAAAA TTTCCTGTTA ATCGAAACTT AACCCGGAAG 
GCTGGATACC TTAATGCTAG GAATAAAACA GGTTTGGTGT CATCTACCTG GGACAGACAA 
TTTTACTTCA CGCAGGGTGG AAACTTAATG AGTCAGGCAC GTGGGGATGT AGCAGGCGGC 
CTGGCCATGG ACATTGACAA CTGTTCAGTA ATGGCTGTGG ACTGTGAAGA CAGGCGGTAC 
TGTTTTCAGA TCACTTCCTT TGATGGAAAA AAGTCTTCAA TTTTACAAGC AGAAAGTAAA 
AAAGACCATG AGGAGTGGAT CTGTACAATA AACAACATAT CCAAACAAAT ATATTTAAGT 
GAAAACCCAG AGGAAATTGC TGCACGAGTA AATCAGTCAG CTTTGGAAGC GGTCACTCCC 
TCTCCATCTT TCCAGCAGAG GCACGAGAGT TTGCGGCCAG CAGCACAATC TCGGCCACCA 
ACAGCTCGAA CCAGCAGCTC AGGATCCTTA GGATCTGAGT CCACAAACTT GGCTGCCCTT 
TCTCTAGATT CTCTCGTTGC TCCAGACACT CCAATACAAT TTGACATCAT TTCTCCCGTG 
TGTGAAGATC AGCCTGGCCA GGCAAAAGCC TTTGGCCAGG GCGGCAGGCG CACAAATCCA 
TTTGGAGAAT CTGGAGGAGG TACAAAATCT GAAACTGAAG ATTCTATTCT TCATCAGTTA 
TTTATCGTGC GATTCCTTGG TTCTATGGAG GTGAAATCAG ACGACAGTCC AGATGTTGTT 
TATGAAACAA TGCGGCAAAT CTTAGCTGCC CGGGCCATCC ATAACATCTT TCGTATGACA 
GAATCACATT TATTAGTCAC TTGTGATTGT TTAAAGTTAA TTGATCCACA GACACAACTT 
ACAAGGCTCA CATTTCCATT ACCCTGTGTA GTTTTGTACG CTACACACCA GGAAAATAAG 
AGGCTTTTTG GATTTGTTCT TCGGACATCA GGAGGGAGAA GTGAAAGTAA TCTGTCATCA 
GTCTGCTATA TATTTGAATC AAATAATGAA GGGGAAAAGA TTTGTGATTC TGTTGGACTG 
GCAAAACAGA TAGCCTTGCA TGCAGAACTG GATCGTAGGG CATCAGAAAA ACAAAAAGAA 
ATGGAGAGGG TAAAAGAGAA GCAACAGAAA GAACTCAGTA AACAAAAACA AATTGAAAAG 
GACTTAGAAG AACAGAGTCG CTTGATAGCT GCTTCCAGTA GACCGAACCA AGCCAGTAGT 
GAGGGGCAGT TTGTTGTCCT TAGCAGTAGC CAGTCAGAAG AGAGTGATTT GGGAGAAGAA 
GGAAAGAAAA GAGAGTCAGA AGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APPL1 ( XM_022406559.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022262267.1 Canis lupus familiaris adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe21522
Clone ID Related Accession (Same CDS sequence)	XM_022406559.1
Accession Version	XM_022406559.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1797bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	DCC-interacting protein 13-alpha isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTTCC CCATTACCCA ATTTAAAGAA AGAGATCTGA AAGAAATACT GACATTAAAG 
GAAGTGTTTC AGATTGCTAG TAATGACCAT GATGCTGCAA TTAACAGATA TAGCCGGTTA 
TCAAAAAAAA GAGAAAATGA CAAGGTAAAG TATGAAGTAA CAGAAGATGT CTACACTTCC 
AGAAAAAAAC AACACCAGAC CATGATGCAT TATTTTTGTG CATTAAACAC TCTTCAATAC 
AAAAAGAAAA TAGCATTGTT AGAACCTCTA CTTGGGTACA TGCAAGCTCA GATAAGTTTC 
TTTAAGATGG GTTCTGAAAA TCTTAATGAA CAGCTGGAAG AATTCTTGAC CAATATTGGA 
ACAAGTGTAC AGAATGTTCG CAGGGAAATG GACAGTGATG TAGAGACCAT GCAGCAGACA 
ATAGAAGATT TGGAAGTAGC CAGTGACCCC TTATATGTGC CTGACCCAGA TCCCACAAAA 
TTTCCTGTTA ATCGAAACTT AACCCGGAAG GCTGGATACC TTAATGCTAG GAATAAAACA 
GGTTTGGTGT CATCTACCTG GGACAGACAA TTTTACTTCA CGCAGGGTGG AAACTTAATG 
AGTCAGGCAC GTGGGGATGT AGCAGGCGGC CTGGCCATGG ACATTGACAA CTGTTCAGTA 
ATGGCTGTGG ACTGTGAAGA CAGGCGGTAC TGTTTTCAGA TCACTTCCTT TGATGGAAAA 
AAGTCTTCAA TTTTACAAGC AGAAAGTAAA AAAGACCATG AGGAGTGGAT CTGTACAATA 
AACAACATAT CCAAACAAAT ATATTTAAGT GAAAACCCAG AGGAAATTGC TGCACGAGTA 
AATCAGTCAG CTTTGGAAGC GGTCACTCCC TCTCCATCTT TCCAGCAGAG GCACGAGAGT 
TTGCGGCCAG CAGCACAATC TCGGCCACCA ACAGCTCGAA CCAGCAGCTC AGGATCCTTA 
GGATCTGAGT CCACAAACTT GGCTGCCCTT TCTCTAGATT CTCTCGTTGC TCCAGACACT 
CCAATACAAT TTGACATCAT TTCTCCCGTG TGTGAAGATC AGCCTGGCCA GGCAAAAGCC 
TTTGGCCAGG GCGGCAGGCG CACAAATCCA TTTGGAGAAT CTGGAGGAGG TACAAAATCT 
GAAACTGAAG ATTCTATTCT TCATCAGTTA TTTATCGTGC GATTCCTTGG TTCTATGGAG 
GTGAAATCAG ACGACAGTCC AGATGTTGTT TATGAAACAA TGCGGCAAAT CTTAGCTGCC 
CGGGCCATCC ATAACATCTT TCGTATGACA GAATCACATT TATTAGTCAC TTGTGATTGT 
TTAAAGTTAA TTGATCCACA GACACAACTT ACAAGGCTCA CATTTCCATT ACCCTGTGTA 
GTTTTGTACG CTACACACCA GGAAAATAAG AGGCTTTTTG GATTTGTTCT TCGGACATCA 
GGAGGGAGAA GTGAAAGTAA TCTGTCATCA GTCTGCTATA TATTTGAATC AAATAATGAA 
GGGGAAAAGA TTTGTGATTC TGTTGGACTG GCAAAACAGA TAGCCTTGCA TGCAGAACTG 
GATCGTAGGG CATCAGAAAA ACAAAAAGAA ATGGAGAGGG TAAAAGAGAA GCAACAGAAA 
GAACTCAGTA AACAAAAACA AATTGAAAAG GACTTAGAAG AACAGAGTCG CTTGATAGCT 
GCTTCCAGTA GACCGAACCA AGCCAGTAGT GAGGGGCAGT TTGTTGTCCT TAGCAGTAGC 
CAGTCAGAAG AGAGTGATTT GGGAGAAGAA GGAAAGAAAA GAGAGTCAGA AGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022262267.1
CDS	185..1981
Translation	MMFPITQFKERDLKEILTLKEVFQIASNDHDAAINRYSRLSKKRENDKVKYEVTEDVYTSRKKQHQTMMHYFCALNTLQYKKKIALLEPLLGYMQAQISFFKMGSENLNEQLEEFLTNIGTSVQNVRREMDSDVETMQQTIEDLEVASDPLYVPDPDPTKFPVNRNLTRKAGYLNARNKTGLVSSTWDRQFYFTQGGNLMSQARGDVAGGLAMDIDNCSVMAVDCEDRRYCFQITSFDGKKSSILQAESKKDHEEWICTINNISKQIYLSENPEEIAARVNQSALEAVTPSPSFQQRHESLRPAAQSRPPTARTSSSGSLGSESTNLAALSLDSLVAPDTPIQFDIISPVCEDQPGQAKAFGQGGRRTNPFGESGGGTKSETEDSILHQLFIVRFLGSMEVKSDDSPDVVYETMRQILAARAIHNIFRMTESHLLVTCDCLKLIDPQTQLTRLTFPLPCVVLYATHQENKRLFGFVLRTSGGRSESNLSSVCYIFESNNEGEKICDSVGLAKQIALHAELDRRASEKQKEMERVKEKQQKELSKQKQIEKDLEEQSRLIAASSRPNQASSEGQFVVLSSSQSEESDLGEEGKKRESEA

Target ORF information:

RefSeq Version	XM_022406559.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022406559.1

ORF Insert Sequence:

ATGATGTTCC CCATTACCCA ATTTAAAGAA AGAGATCTGA AAGAAATACT GACATTAAAG 
GAAGTGTTTC AGATTGCTAG TAATGACCAT GATGCTGCAA TTAACAGATA TAGCCGGTTA 
TCAAAAAAAA GAGAAAATGA CAAGGTAAAG TATGAAGTAA CAGAAGATGT CTACACTTCC 
AGAAAAAAAC AACACCAGAC CATGATGCAT TATTTTTGTG CATTAAACAC TCTTCAATAC 
AAAAAGAAAA TAGCATTGTT AGAACCTCTA CTTGGGTACA TGCAAGCTCA GATAAGTTTC 
TTTAAGATGG GTTCTGAAAA TCTTAATGAA CAGCTGGAAG AATTCTTGAC CAATATTGGA 
ACAAGTGTAC AGAATGTTCG CAGGGAAATG GACAGTGATG TAGAGACCAT GCAGCAGACA 
ATAGAAGATT TGGAAGTAGC CAGTGACCCC TTATATGTGC CTGACCCAGA TCCCACAAAA 
TTTCCTGTTA ATCGAAACTT AACCCGGAAG GCTGGATACC TTAATGCTAG GAATAAAACA 
GGTTTGGTGT CATCTACCTG GGACAGACAA TTTTACTTCA CGCAGGGTGG AAACTTAATG 
AGTCAGGCAC GTGGGGATGT AGCAGGCGGC CTGGCCATGG ACATTGACAA CTGTTCAGTA 
ATGGCTGTGG ACTGTGAAGA CAGGCGGTAC TGTTTTCAGA TCACTTCCTT TGATGGAAAA 
AAGTCTTCAA TTTTACAAGC AGAAAGTAAA AAAGACCATG AGGAGTGGAT CTGTACAATA 
AACAACATAT CCAAACAAAT ATATTTAAGT GAAAACCCAG AGGAAATTGC TGCACGAGTA 
AATCAGTCAG CTTTGGAAGC GGTCACTCCC TCTCCATCTT TCCAGCAGAG GCACGAGAGT 
TTGCGGCCAG CAGCACAATC TCGGCCACCA ACAGCTCGAA CCAGCAGCTC AGGATCCTTA 
GGATCTGAGT CCACAAACTT GGCTGCCCTT TCTCTAGATT CTCTCGTTGC TCCAGACACT 
CCAATACAAT TTGACATCAT TTCTCCCGTG TGTGAAGATC AGCCTGGCCA GGCAAAAGCC 
TTTGGCCAGG GCGGCAGGCG CACAAATCCA TTTGGAGAAT CTGGAGGAGG TACAAAATCT 
GAAACTGAAG ATTCTATTCT TCATCAGTTA TTTATCGTGC GATTCCTTGG TTCTATGGAG 
GTGAAATCAG ACGACAGTCC AGATGTTGTT TATGAAACAA TGCGGCAAAT CTTAGCTGCC 
CGGGCCATCC ATAACATCTT TCGTATGACA GAATCACATT TATTAGTCAC TTGTGATTGT 
TTAAAGTTAA TTGATCCACA GACACAACTT ACAAGGCTCA CATTTCCATT ACCCTGTGTA 
GTTTTGTACG CTACACACCA GGAAAATAAG AGGCTTTTTG GATTTGTTCT TCGGACATCA 
GGAGGGAGAA GTGAAAGTAA TCTGTCATCA GTCTGCTATA TATTTGAATC AAATAATGAA 
GGGGAAAAGA TTTGTGATTC TGTTGGACTG GCAAAACAGA TAGCCTTGCA TGCAGAACTG 
GATCGTAGGG CATCAGAAAA ACAAAAAGAA ATGGAGAGGG TAAAAGAGAA GCAACAGAAA 
GAACTCAGTA AACAAAAACA AATTGAAAAG GACTTAGAAG AACAGAGTCG CTTGATAGCT 
GCTTCCAGTA GACCGAACCA AGCCAGTAGT GAGGGGCAGT TTGTTGTCCT TAGCAGTAGC 
CAGTCAGAAG AGAGTGATTT GGGAGAAGAA GGAAAGAAAA GAGAGTCAGA AGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APPL1 ( XM_022406557.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022262265.1 Canis lupus familiaris adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe295355
Clone ID Related Accession (Same CDS sequence)	XM_022406557.1
Accession Version	XM_022406557.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	DCC-interacting protein 13-alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGAA TCGACAAGCT GCCCATCGAG GAGACGCTGG AGGACAGCCC GCAGACAAGG 
TCTTTACTGG GTGTATTTGA AGAAGATGCT ACAGCTATTT CCAACTATAT GAACCAGTTG 
TATCAAGCTA TGCGTCGGAT TTATGATGCA CAGAATGAAT TAAGTGCAGC AACACATCTG 
ACTTCAAAAC TTTTAAAAGA ATATGAAAAA CAGCGGTTTC CATTGGGGGG TGATGATGAA 
GTTATGAGCT CTACTTTACA GCAGTTTTCA AAAGTTATAG ATGAGCTTAG CTCTTGTCAT 
GCAGTACTTT CAACGCAGCT TGCTGATGCC ATGATGTTCC CCATTACCCA ATTTAAAGAA 
AGAGATCTGA AAGAAATACT GACATTAAAG GAAGTGTTTC AGATTGCTAG TAATGACCAT 
GATGCTGCAA TTAACAGATA TAGCCGGTTA TCAAAAAAAA GAGAAAATGA CAAGGTAAAG 
TATGAAGTAA CAGAAGATGT CTACACTTCC AGAAAAAAAC AACACCAGAC CATGATGCAT 
TATTTTTGTG CATTAAACAC TCTTCAATAC AAAAAGAAAA TAGCATTGTT AGAACCTCTA 
CTTGGGTACA TGCAAGCTCA GATAAGTTTC TTTAAGATGG GTTCTGAAAA TCTTAATGAA 
CAGCTGGAAG AATTCTTGAC CAATATTGGA ACAAGTGTAC AGAATGTTCG CAGGGAAATG 
GACAGTGATG TAGAGACCAT GCAGCAGACA ATAGAAGATT TGGAAGTAGC CAGTGACCCC 
TTATATGTGC CTGACCCAGA TCCCACAAAA TTTCCTGTTA ATCGAAACTT AACCCGGAAG 
GCTGGATACC TTAATGCTAG GAAGTCTTCA ATTTTACAAG CAGAAAGTAA AAAAGACCAT 
GAGGAGTGGA TCTGTACAAT AAACAACATA TCCAAACAAA TATATTTAAG TGAAAACCCA 
GAGGAAATTG CTGCACGAGT AAATCAGTCA GCTTTGGAAG CGGTCACTCC CTCTCCATCT 
TTCCAGCAGA GGCACGAGAG TTTGCGGCCA GCAGCACAAT CTCGGCCACC AACAGCTCGA 
ACCAGCAGCT CAGGATCCTT AGGATCTGAG TCCACAAACT TGGCTGCCCT TTCTCTAGAT 
TCTCTCGTTG CTCCAGACAC TCCAATACAA TTTGACATCA TTTCTCCCGT GTGTGAAGAT 
CAGCCTGGCC AGGCAAAAGC CTTTGGCCAG GGCGGCAGGC GCACAAATCC ATTTGGAGAA 
TCTGGAGGAG GTACAAAATC TGAAACTGAA GATTCTATTC TTCATCAGTT ATTTATCGTG 
CGATTCCTTG GTTCTATGGA GGTGAAATCA GACGACAGTC CAGATGTTGT TTATGAAACA 
ATGCGGCAAA TCTTAGCTGC CCGGGCCATC CATAACATCT TTCGTATGAC AGAATCACAT 
TTATTAGTCA CTTGTGATTG TTTAAAGTTA ATTGATCCAC AGACACAACT TACAAGGCTC 
ACATTTCCAT TACCCTGTGT AGTTTTGTAC GCTACACACC AGGAAAATAA GAGGCTTTTT 
GGATTTGTTC TTCGGACATC AGGAGGGAGA AGTGAAAGTA ATCTGTCATC AGTCTGCTAT 
ATATTTGAAT CAAATAATGA AGGGGAAAAG ATTTGTGATT CTGTTGGACT GGCAAAACAG 
ATAGCCTTGC ATGCAGAACT GGATCGTAGG GCATCAGAAA AACAAAAAGA AATGGAGAGG 
GTAAAAGAGA AGCAACAGAA AGAACTCAGT AAACAAAAAC AAATTGAAAA GGACTTAGAA 
GAACAGAGTC GCTTGATAGC TGCTTCCAGT AGACCGAACC AAGCCAGTAG TGAGGGGCAG 
TTTGTTGTCC TTAGCAGTAG CCAGTCAGAA GAGAGTGATT TGGGAGAAGA AGGAAAGAAA 
AGAGAGTCAG AAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022262265.1
CDS	122..2059
Translation	MPGIDKLPIEETLEDSPQTRSLLGVFEEDATAISNYMNQLYQAMRRIYDAQNELSAATHLTSKLLKEYEKQRFPLGGDDEVMSSTLQQFSKVIDELSSCHAVLSTQLADAMMFPITQFKERDLKEILTLKEVFQIASNDHDAAINRYSRLSKKRENDKVKYEVTEDVYTSRKKQHQTMMHYFCALNTLQYKKKIALLEPLLGYMQAQISFFKMGSENLNEQLEEFLTNIGTSVQNVRREMDSDVETMQQTIEDLEVASDPLYVPDPDPTKFPVNRNLTRKAGYLNARKSSILQAESKKDHEEWICTINNISKQIYLSENPEEIAARVNQSALEAVTPSPSFQQRHESLRPAAQSRPPTARTSSSGSLGSESTNLAALSLDSLVAPDTPIQFDIISPVCEDQPGQAKAFGQGGRRTNPFGESGGGTKSETEDSILHQLFIVRFLGSMEVKSDDSPDVVYETMRQILAARAIHNIFRMTESHLLVTCDCLKLIDPQTQLTRLTFPLPCVVLYATHQENKRLFGFVLRTSGGRSESNLSSVCYIFESNNEGEKICDSVGLAKQIALHAELDRRASEKQKEMERVKEKQQKELSKQKQIEKDLEEQSRLIAASSRPNQASSEGQFVVLSSSQSEESDLGEEGKKRESEA

Target ORF information:

RefSeq Version	XM_022406557.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022406557.1

ORF Insert Sequence:

ATGCCGGGAA TCGACAAGCT GCCCATCGAG GAGACGCTGG AGGACAGCCC GCAGACAAGG 
TCTTTACTGG GTGTATTTGA AGAAGATGCT ACAGCTATTT CCAACTATAT GAACCAGTTG 
TATCAAGCTA TGCGTCGGAT TTATGATGCA CAGAATGAAT TAAGTGCAGC AACACATCTG 
ACTTCAAAAC TTTTAAAAGA ATATGAAAAA CAGCGGTTTC CATTGGGGGG TGATGATGAA 
GTTATGAGCT CTACTTTACA GCAGTTTTCA AAAGTTATAG ATGAGCTTAG CTCTTGTCAT 
GCAGTACTTT CAACGCAGCT TGCTGATGCC ATGATGTTCC CCATTACCCA ATTTAAAGAA 
AGAGATCTGA AAGAAATACT GACATTAAAG GAAGTGTTTC AGATTGCTAG TAATGACCAT 
GATGCTGCAA TTAACAGATA TAGCCGGTTA TCAAAAAAAA GAGAAAATGA CAAGGTAAAG 
TATGAAGTAA CAGAAGATGT CTACACTTCC AGAAAAAAAC AACACCAGAC CATGATGCAT 
TATTTTTGTG CATTAAACAC TCTTCAATAC AAAAAGAAAA TAGCATTGTT AGAACCTCTA 
CTTGGGTACA TGCAAGCTCA GATAAGTTTC TTTAAGATGG GTTCTGAAAA TCTTAATGAA 
CAGCTGGAAG AATTCTTGAC CAATATTGGA ACAAGTGTAC AGAATGTTCG CAGGGAAATG 
GACAGTGATG TAGAGACCAT GCAGCAGACA ATAGAAGATT TGGAAGTAGC CAGTGACCCC 
TTATATGTGC CTGACCCAGA TCCCACAAAA TTTCCTGTTA ATCGAAACTT AACCCGGAAG 
GCTGGATACC TTAATGCTAG GAAGTCTTCA ATTTTACAAG CAGAAAGTAA AAAAGACCAT 
GAGGAGTGGA TCTGTACAAT AAACAACATA TCCAAACAAA TATATTTAAG TGAAAACCCA 
GAGGAAATTG CTGCACGAGT AAATCAGTCA GCTTTGGAAG CGGTCACTCC CTCTCCATCT 
TTCCAGCAGA GGCACGAGAG TTTGCGGCCA GCAGCACAAT CTCGGCCACC AACAGCTCGA 
ACCAGCAGCT CAGGATCCTT AGGATCTGAG TCCACAAACT TGGCTGCCCT TTCTCTAGAT 
TCTCTCGTTG CTCCAGACAC TCCAATACAA TTTGACATCA TTTCTCCCGT GTGTGAAGAT 
CAGCCTGGCC AGGCAAAAGC CTTTGGCCAG GGCGGCAGGC GCACAAATCC ATTTGGAGAA 
TCTGGAGGAG GTACAAAATC TGAAACTGAA GATTCTATTC TTCATCAGTT ATTTATCGTG 
CGATTCCTTG GTTCTATGGA GGTGAAATCA GACGACAGTC CAGATGTTGT TTATGAAACA 
ATGCGGCAAA TCTTAGCTGC CCGGGCCATC CATAACATCT TTCGTATGAC AGAATCACAT 
TTATTAGTCA CTTGTGATTG TTTAAAGTTA ATTGATCCAC AGACACAACT TACAAGGCTC 
ACATTTCCAT TACCCTGTGT AGTTTTGTAC GCTACACACC AGGAAAATAA GAGGCTTTTT 
GGATTTGTTC TTCGGACATC AGGAGGGAGA AGTGAAAGTA ATCTGTCATC AGTCTGCTAT 
ATATTTGAAT CAAATAATGA AGGGGAAAAG ATTTGTGATT CTGTTGGACT GGCAAAACAG 
ATAGCCTTGC ATGCAGAACT GGATCGTAGG GCATCAGAAA AACAAAAAGA AATGGAGAGG 
GTAAAAGAGA AGCAACAGAA AGAACTCAGT AAACAAAAAC AAATTGAAAA GGACTTAGAA 
GAACAGAGTC GCTTGATAGC TGCTTCCAGT AGACCGAACC AAGCCAGTAG TGAGGGGCAG 
TTTGTTGTCC TTAGCAGTAG CCAGTCAGAA GAGAGTGATT TGGGAGAAGA AGGAAAGAAA 
AGAGAGTCAG AAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APPL1 ( XM_022406558.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022262266.1 Canis lupus familiaris adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe295356
Clone ID Related Accession (Same CDS sequence)	XM_022406558.1
Accession Version	XM_022406558.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	DCC-interacting protein 13-alpha isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTGC ATGTTTTGGA TATTTCATAT AAATGGACTT ATCTTAGCTC TTGTCATGCA 
GTACTTTCAA CGCAGCTTGC TGATGCCATG ATGTTCCCCA TTACCCAATT TAAAGAAAGA 
GATCTGAAAG AAATACTGAC ATTAAAGGAA GTGTTTCAGA TTGCTAGTAA TGACCATGAT 
GCTGCAATTA ACAGATATAG CCGGTTATCA AAAAAAAGAG AAAATGACAA GGTAAAGTAT 
GAAGTAACAG AAGATGTCTA CACTTCCAGA AAAAAACAAC ACCAGACCAT GATGCATTAT 
TTTTGTGCAT TAAACACTCT TCAATACAAA AAGAAAATAG CATTGTTAGA ACCTCTACTT 
GGGTACATGC AAGCTCAGAT AAGTTTCTTT AAGATGGGTT CTGAAAATCT TAATGAACAG 
CTGGAAGAAT TCTTGACCAA TATTGGAACA AGTGTACAGA ATGTTCGCAG GGAAATGGAC 
AGTGATGTAG AGACCATGCA GCAGACAATA GAAGATTTGG AAGTAGCCAG TGACCCCTTA 
TATGTGCCTG ACCCAGATCC CACAAAATTT CCTGTTAATC GAAACTTAAC CCGGAAGGCT 
GGATACCTTA ATGCTAGGAA TAAAACAGGT TTGGTGTCAT CTACCTGGGA CAGACAATTT 
TACTTCACGC AGGGTGGAAA CTTAATGAGT CAGGCACGTG GGGATGTAGC AGGCGGCCTG 
GCCATGGACA TTGACAACTG TTCAGTAATG GCTGTGGACT GTGAAGACAG GCGGTACTGT 
TTTCAGATCA CTTCCTTTGA TGGAAAAAAG TCTTCAATTT TACAAGCAGA AAGTAAAAAA 
GACCATGAGG AGTGGATCTG TACAATAAAC AACATATCCA AACAAATATA TTTAAGTGAA 
AACCCAGAGG AAATTGCTGC ACGAGTAAAT CAGTCAGCTT TGGAAGCGGT CACTCCCTCT 
CCATCTTTCC AGCAGAGGCA CGAGAGTTTG CGGCCAGCAG CACAATCTCG GCCACCAACA 
GCTCGAACCA GCAGCTCAGG ATCCTTAGGA TCTGAGTCCA CAAACTTGGC TGCCCTTTCT 
CTAGATTCTC TCGTTGCTCC AGACACTCCA ATACAATTTG ACATCATTTC TCCCGTGTGT 
GAAGATCAGC CTGGCCAGGC AAAAGCCTTT GGCCAGGGCG GCAGGCGCAC AAATCCATTT 
GGAGAATCTG GAGGAGGTAC AAAATCTGAA ACTGAAGATT CTATTCTTCA TCAGTTATTT 
ATCGTGCGAT TCCTTGGTTC TATGGAGGTG AAATCAGACG ACAGTCCAGA TGTTGTTTAT 
GAAACAATGC GGCAAATCTT AGCTGCCCGG GCCATCCATA ACATCTTTCG TATGACAGAA 
TCACATTTAT TAGTCACTTG TGATTGTTTA AAGTTAATTG ATCCACAGAC ACAACTTACA 
AGGCTCACAT TTCCATTACC CTGTGTAGTT TTGTACGCTA CACACCAGGA AAATAAGAGG 
CTTTTTGGAT TTGTTCTTCG GACATCAGGA GGGAGAAGTG AAAGTAATCT GTCATCAGTC 
TGCTATATAT TTGAATCAAA TAATGAAGGG GAAAAGATTT GTGATTCTGT TGGACTGGCA 
AAACAGATAG CCTTGCATGC AGAACTGGAT CGTAGGGCAT CAGAAAAACA AAAAGAAATG 
GAGAGGGTAA AAGAGAAGCA ACAGAAAGAA CTCAGTAAAC AAAAACAAAT TGAAAAGGAC 
TTAGAAGAAC AGAGTCGCTT GATAGCTGCT TCCAGTAGAC CGAACCAAGC CAGTAGTGAG 
GGGCAGTTTG TTGTCCTTAG CAGTAGCCAG TCAGAAGAGA GTGATTTGGG AGAAGAAGGA 
AAGAAAAGAG AGTCAGAAGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022262266.1
CDS	16..1899
Translation	MDLHVLDISYKWTYLSSCHAVLSTQLADAMMFPITQFKERDLKEILTLKEVFQIASNDHDAAINRYSRLSKKRENDKVKYEVTEDVYTSRKKQHQTMMHYFCALNTLQYKKKIALLEPLLGYMQAQISFFKMGSENLNEQLEEFLTNIGTSVQNVRREMDSDVETMQQTIEDLEVASDPLYVPDPDPTKFPVNRNLTRKAGYLNARNKTGLVSSTWDRQFYFTQGGNLMSQARGDVAGGLAMDIDNCSVMAVDCEDRRYCFQITSFDGKKSSILQAESKKDHEEWICTINNISKQIYLSENPEEIAARVNQSALEAVTPSPSFQQRHESLRPAAQSRPPTARTSSSGSLGSESTNLAALSLDSLVAPDTPIQFDIISPVCEDQPGQAKAFGQGGRRTNPFGESGGGTKSETEDSILHQLFIVRFLGSMEVKSDDSPDVVYETMRQILAARAIHNIFRMTESHLLVTCDCLKLIDPQTQLTRLTFPLPCVVLYATHQENKRLFGFVLRTSGGRSESNLSSVCYIFESNNEGEKICDSVGLAKQIALHAELDRRASEKQKEMERVKEKQQKELSKQKQIEKDLEEQSRLIAASSRPNQASSEGQFVVLSSSQSEESDLGEEGKKRESEA

Target ORF information:

RefSeq Version	XM_022406558.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022406558.1

ORF Insert Sequence:

ATGGATTTGC ATGTTTTGGA TATTTCATAT AAATGGACTT ATCTTAGCTC TTGTCATGCA 
GTACTTTCAA CGCAGCTTGC TGATGCCATG ATGTTCCCCA TTACCCAATT TAAAGAAAGA 
GATCTGAAAG AAATACTGAC ATTAAAGGAA GTGTTTCAGA TTGCTAGTAA TGACCATGAT 
GCTGCAATTA ACAGATATAG CCGGTTATCA AAAAAAAGAG AAAATGACAA GGTAAAGTAT 
GAAGTAACAG AAGATGTCTA CACTTCCAGA AAAAAACAAC ACCAGACCAT GATGCATTAT 
TTTTGTGCAT TAAACACTCT TCAATACAAA AAGAAAATAG CATTGTTAGA ACCTCTACTT 
GGGTACATGC AAGCTCAGAT AAGTTTCTTT AAGATGGGTT CTGAAAATCT TAATGAACAG 
CTGGAAGAAT TCTTGACCAA TATTGGAACA AGTGTACAGA ATGTTCGCAG GGAAATGGAC 
AGTGATGTAG AGACCATGCA GCAGACAATA GAAGATTTGG AAGTAGCCAG TGACCCCTTA 
TATGTGCCTG ACCCAGATCC CACAAAATTT CCTGTTAATC GAAACTTAAC CCGGAAGGCT 
GGATACCTTA ATGCTAGGAA TAAAACAGGT TTGGTGTCAT CTACCTGGGA CAGACAATTT 
TACTTCACGC AGGGTGGAAA CTTAATGAGT CAGGCACGTG GGGATGTAGC AGGCGGCCTG 
GCCATGGACA TTGACAACTG TTCAGTAATG GCTGTGGACT GTGAAGACAG GCGGTACTGT 
TTTCAGATCA CTTCCTTTGA TGGAAAAAAG TCTTCAATTT TACAAGCAGA AAGTAAAAAA 
GACCATGAGG AGTGGATCTG TACAATAAAC AACATATCCA AACAAATATA TTTAAGTGAA 
AACCCAGAGG AAATTGCTGC ACGAGTAAAT CAGTCAGCTT TGGAAGCGGT CACTCCCTCT 
CCATCTTTCC AGCAGAGGCA CGAGAGTTTG CGGCCAGCAG CACAATCTCG GCCACCAACA 
GCTCGAACCA GCAGCTCAGG ATCCTTAGGA TCTGAGTCCA CAAACTTGGC TGCCCTTTCT 
CTAGATTCTC TCGTTGCTCC AGACACTCCA ATACAATTTG ACATCATTTC TCCCGTGTGT 
GAAGATCAGC CTGGCCAGGC AAAAGCCTTT GGCCAGGGCG GCAGGCGCAC AAATCCATTT 
GGAGAATCTG GAGGAGGTAC AAAATCTGAA ACTGAAGATT CTATTCTTCA TCAGTTATTT 
ATCGTGCGAT TCCTTGGTTC TATGGAGGTG AAATCAGACG ACAGTCCAGA TGTTGTTTAT 
GAAACAATGC GGCAAATCTT AGCTGCCCGG GCCATCCATA ACATCTTTCG TATGACAGAA 
TCACATTTAT TAGTCACTTG TGATTGTTTA AAGTTAATTG ATCCACAGAC ACAACTTACA 
AGGCTCACAT TTCCATTACC CTGTGTAGTT TTGTACGCTA CACACCAGGA AAATAAGAGG 
CTTTTTGGAT TTGTTCTTCG GACATCAGGA GGGAGAAGTG AAAGTAATCT GTCATCAGTC 
TGCTATATAT TTGAATCAAA TAATGAAGGG GAAAAGATTT GTGATTCTGT TGGACTGGCA 
AAACAGATAG CCTTGCATGC AGAACTGGAT CGTAGGGCAT CAGAAAAACA AAAAGAAATG 
GAGAGGGTAA AAGAGAAGCA ACAGAAAGAA CTCAGTAAAC AAAAACAAAT TGAAAAGGAC 
TTAGAAGAAC AGAGTCGCTT GATAGCTGCT TCCAGTAGAC CGAACCAAGC CAGTAGTGAG 
GGGCAGTTTG TTGTCCTTAG CAGTAGCCAG TCAGAAGAGA GTGATTTGGG AGAAGAAGGA 
AAGAAAAGAG AGTCAGAAGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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APPL1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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