ACTN3 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ACTN3
Entrez Gene ID	483707
Full Name	actinin alpha 3
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005631429.3	XP_005631486.2	alpha-actinin-3 isoform X1
XM_847243.4	XP_852336.1	alpha-actinin-3 isoform X2
XM_022405619.1	XP_022261327.1	alpha-actinin-3 isoform X3
XM_005631429.2	XP_005631486.1	alpha-actinin-3 isoform X1
XM_847243.5	XP_852336.2	alpha-actinin-3 isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ACTN3	XP_005631486.1
Anopheles gambiae (African malaria mosquito)	ACTN_ANOGA	XP_321625.2
Bos taurus (cattle)	ACTN3	NP_001069625.1
Mus musculus (house mouse)	Actn3	NP_038484.1
Pan troglodytes (chimpanzee)	ACTN3	XP_003313215.1
	ACTN3	XP_001109839.1
Danio rerio (zebrafish)	actn3b	NP_991107.1
Drosophila melanogaster (fruit fly)	Actn	NP_477485.1
Rattus norvegicus (Norway rat)	Actn3	NP_596915.1
Danio rerio (zebrafish)	actn3a	NP_571597.1
Caenorhabditis elegans (roundworm)	atn-1	NP_506128.1
Xenopus tropicalis (tropical clawed frog)	actn3	NP_001135513.1

Gene Ontology

The following ACTN3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACTN3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe20302	XM_005631429.2 Latest version!	Canis lupus familiaris actinin, alpha 3 (ACTN3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe20303	XM_847243.4 Latest version!	Canis lupus familiaris actinin, alpha 3 (ACTN3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe294888	XM_022405619.1 Latest version!	Canis lupus familiaris actinin alpha 3 (ACTN3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OCe294886	XM_005631429.3 Latest version!	Canis lupus familiaris actinin alpha 3 (ACTN3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe294887	XM_847243.5 Latest version!	Canis lupus familiaris actinin alpha 3 (ACTN3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ACTN3 ( XM_005631429.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005631486.1 Canis lupus familiaris actinin, alpha 3 (ACTN3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe20302
Clone ID Related Accession (Same CDS sequence)	XM_005631429.2
Accession Version	XM_005631429.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2793bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	alpha-actinin-3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005631429.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGG TTATGCAGCC CGAGGGCCTG GGGGTCGGGG AGGGGCCCTT CGTGGGCGGC 
AGCGGGGGCG GCGAGTACAT GGAACAGGAG GAAGACTGGG ACCGCGACCT ACTGCTCGAC 
CCGGCCTGGG AGAAGCAGCA GCGGAAAACC TTCACTGCCT GGTGCAACTC ACACCTGCGC 
AAGGCTGGCA CCCAGATTGA AAACATAGAG GAGGATTTCC GCAATGGCCT CAAACTCATG 
TTGCTTTTGG AGGTCATTTC AGGAGAGAGG CTACCTAGGC CAGACAAAGG CAAGATGCGC 
TTCCACAAAA TTGCCAATGT CAACAAGGCC CTGGACTTCA TTGCCAGCAA GGGGGTTAAG 
CTGGTGTCCA TTGGTGCTGA AGAGATTGTT GACGGGAACC TGAAGATGAC CCTGGGCATG 
ATCTGGACCA TCATCCTTCG CTTTGCTATC CAGGACATCT CTGTGGAGGA AACCTCTGCC 
AAGGAAGGCT TGCTCCTGTG GTGCCAGCGG AAGACAGCTC CGTACCGCAA CGTCAACGTG 
CAAAACTTCC ACACCAGCTG GAAGGATGGT TTGGCCCTCT GTGCTCTCAT CCACCGACAC 
CGCCCCGATC TCATCGATTA TGCCAAACTA CGCAAGGATG ACCCCATTGG CAATCTAAAC 
ACGGCCTTCG AGGTTGCAGA GAAATACTTG GACATCCCTA GGATGTTGGA TGCAGAAGAC 
ATCGTGAACA CCCCAAAGCC TGATGAAAAG GCCATCATGA CCTATGTTTC CTGCTTCTAC 
CATGCCTTTG CCGGGGCTGA GCAGGCAGAG ACAGCTGCCA ACAGGATCTG CAAGGTGCTG 
GCTGTGAACC AGGAGAACGA GAAGCTGATG GAGGAGTACG AGAAGCTTGC CAGTGAGCTG 
CTGGAATGGA TCCGTCGCAC CATACCATGG CTGGAGAACC GTGTGGGCGA GCCCAGCATG 
AGTGCCATGC AGCGCAAGCT GGAGGACTTT CGGGACTACC GGCGCCTGCA TAAGCCACCC 
CGAGTGCAGG AGAAGTGCCA GCTGGAGATC AATTTCAATA CACTGCAGAC CAAGCTGCGG 
CTGAGCCACC GGCCCGCCTT CATGCCCTCC GAGGGCAAGC TCGTCTCGGA TATCGCCAAT 
GCATGGCGTG GGCTGGAGCA AGTAGAGAAG GGCTATGAGG ACTGGCTGCT CTCAGAGATC 
CGGCGCCTGC AGCGGCTCCA GCACCTGGCT GAGAAATTCC AGCAGAAAGC CTCCTTGCAT 
GAAGCCTGGA CCCGGGGGAA GGAGGATATG CTGAGCCAGC GCGACTACGA GTCGGCCTCC 
CTGCAGGAGG TGCGGGCGTT GCTGCGGCGC CACGAGGCCT TTGAGAGCGA CCTGGCAGCG 
CATCAGGACC GCGTGGAGCA CATCGCCGCG CTGGCCCAGG AGCTCAATGA GCTGGACTAC 
CATGAGGCAG CCTCGGTGAA TAGCCGCTGC CAGGCCATCT GCGACCAGTG GGACAATCTG 
GGCACGCTGA CCCAGAAGAG GAGGGATGCG CTAGAACGGA TGGAGAAGCT CCTGGAGACC 
ATTGACCAGC TGCAACTGGA GTTTGCTCGT CGGGCAGCGC CCTTCAACAA CTGGCTGGAT 
GGTGCCGTGG AGGACCTGCA GGACGTGTGG CTGGTGCACT CAGTGGAGGA AACCCAGAGC 
CTGCTGACTG CACATGAGCA ATTCAAGGCT ACGTTGCCAG AGGCTGACCG AGAGCGAGGA 
GCAATCCTCG GTATCCAGGG TGAGATCCAG AAGATCTGCC AAACGTATGG ACTGCGGCCC 
AGCTCCACCA ACCCCTACAT CAACCTCACA CCACAGGACA TCAACACCAA GTGGGACATG 
GTCCGAAAGC TGGTGCCCAG CCGTGACCAG ACCCTGCAGG AAGAGCTTGC CCGGCAGCAG 
CTGAATGAGA GGCTCCGGCG ACAGTTCGCA GCTCAAGCCA ATGCTGTTGG GCCCTGGATC 
CAGGGGAAGG TGGAGGAGGT GGGGCGCCTA GCCACAGGGA TGGCTGGCTC TCTGGAGGAA 
CAGATGGCTG GACTGCGGCA ACAGGAGCAG AACATCATCA ACTACAAGAG CAACATTGAC 
CGGCTGGAGG GTGACCACCA GCTGCTGCAG GAGAGCCTAG TGTTTGACAA CAAGCACACC 
GTCTACAGCA TGGAGCACAT CCGCGTAGGC TGGGAGCAGC TGCTCACCTC CATCGCCCGT 
ACCATCAATG AAGTGGAGAA CCAGGTACTG ACCCGAGATG CCAAGGGCCT GAGCCAGGAG 
CAGCTCAACG AGTTCCGGGC ATCCTTCAAC CACTTTGACC GGGTCAGCGA GGGCCCAATT 
CTGTGGGCGG TGGATGTGGG GGCTGGTGGG GCTGGAGGAC CAAGTCTGAT ATGCACTCAT 
GCACTACAGA AGCGGAACGG GATGATGGAG CCTGATGACT TCCGGGCTTG CCTCATCTCC 
ATGGGCTATG ATCTGGGAGA AGTAGAGTTT GCACGAATTA TGACCATGGT GGACCCCAAC 
GCAGCCGGCG TCGTGACCTT CCAGGCCTTC ATCGACTTCA TGACCCGAGA GACAGCTGAG 
ACTGACACAG CTGAGCAAGT TGTGGCCTCC TTCAAGATCC TGGCAGGAGA TAAGAACTAC 
ATCACTCCTG AAGAGCTGCG GCGGGAGCTC CCTGCTGAGC AGGCCGAGTA CTGCATCCGT 
CGTATGGCAC CCTACAAGGG CTCTGGGGCT CCACCTGGAG CCCTGGACTA CGTGGCCTTC 
TCCAGTGCCC TCTATGGGGA GAGTGACCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005631486.1
CDS	78..2870
Translation	MMMVMQPEGLGVGEGPFVGGSGGGEYMEQEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRNGLKLMLLLEVISGERLPRPDKGKMRFHKIANVNKALDFIASKGVKLVSIGAEEIVDGNLKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYRNVNVQNFHTSWKDGLALCALIHRHRPDLIDYAKLRKDDPIGNLNTAFEVAEKYLDIPRMLDAEDIVNTPKPDEKAIMTYVSCFYHAFAGAEQAETAANRICKVLAVNQENEKLMEEYEKLASELLEWIRRTIPWLENRVGEPSMSAMQRKLEDFRDYRRLHKPPRVQEKCQLEINFNTLQTKLRLSHRPAFMPSEGKLVSDIANAWRGLEQVEKGYEDWLLSEIRRLQRLQHLAEKFQQKASLHEAWTRGKEDMLSQRDYESASLQEVRALLRRHEAFESDLAAHQDRVEHIAALAQELNELDYHEAASVNSRCQAICDQWDNLGTLTQKRRDALERMEKLLETIDQLQLEFARRAAPFNNWLDGAVEDLQDVWLVHSVEETQSLLTAHEQFKATLPEADRERGAILGIQGEIQKICQTYGLRPSSTNPYINLTPQDINTKWDMVRKLVPSRDQTLQEELARQQLNERLRRQFAAQANAVGPWIQGKVEEVGRLATGMAGSLEEQMAGLRQQEQNIINYKSNIDRLEGDHQLLQESLVFDNKHTVYSMEHIRVGWEQLLTSIARTINEVENQVLTRDAKGLSQEQLNEFRASFNHFDRVSEGPILWAVDVGAGGAGGPSLICTHALQKRNGMMEPDDFRACLISMGYDLGEVEFARIMTMVDPNAAGVVTFQAFIDFMTRETAETDTAEQVVASFKILAGDKNYITPEELRRELPAEQAEYCIRRMAPYKGSGAPPGALDYVAFSSALYGESDL

Target ORF information:

RefSeq Version	XM_005631429.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris actinin, alpha 3 (ACTN3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005631429.2

ORF Insert Sequence:

ATGATGATGG TTATGCAGCC CGAGGGCCTG GGGGTCGGGG AGGGGCCCTT CGTGGGCGGC 
AGCGGGGGCG GCGAGTACAT GGAACAGGAG GAAGACTGGG ACCGCGACCT ACTGCTCGAC 
CCGGCCTGGG AGAAGCAGCA GCGGAAAACC TTCACTGCCT GGTGCAACTC ACACCTGCGC 
AAGGCTGGCA CCCAGATTGA AAACATAGAG GAGGATTTCC GCAATGGCCT CAAACTCATG 
TTGCTTTTGG AGGTCATTTC AGGAGAGAGG CTACCTAGGC CAGACAAAGG CAAGATGCGC 
TTCCACAAAA TTGCCAATGT CAACAAGGCC CTGGACTTCA TTGCCAGCAA GGGGGTTAAG 
CTGGTGTCCA TTGGTGCTGA AGAGATTGTT GACGGGAACC TGAAGATGAC CCTGGGCATG 
ATCTGGACCA TCATCCTTCG CTTTGCTATC CAGGACATCT CTGTGGAGGA AACCTCTGCC 
AAGGAAGGCT TGCTCCTGTG GTGCCAGCGG AAGACAGCTC CGTACCGCAA CGTCAACGTG 
CAAAACTTCC ACACCAGCTG GAAGGATGGT TTGGCCCTCT GTGCTCTCAT CCACCGACAC 
CGCCCCGATC TCATCGATTA TGCCAAACTA CGCAAGGATG ACCCCATTGG CAATCTAAAC 
ACGGCCTTCG AGGTTGCAGA GAAATACTTG GACATCCCTA GGATGTTGGA TGCAGAAGAC 
ATCGTGAACA CCCCAAAGCC TGATGAAAAG GCCATCATGA CCTATGTTTC CTGCTTCTAC 
CATGCCTTTG CCGGGGCTGA GCAGGCAGAG ACAGCTGCCA ACAGGATCTG CAAGGTGCTG 
GCTGTGAACC AGGAGAACGA GAAGCTGATG GAGGAGTACG AGAAGCTTGC CAGTGAGCTG 
CTGGAATGGA TCCGTCGCAC CATACCATGG CTGGAGAACC GTGTGGGCGA GCCCAGCATG 
AGTGCCATGC AGCGCAAGCT GGAGGACTTT CGGGACTACC GGCGCCTGCA TAAGCCACCC 
CGAGTGCAGG AGAAGTGCCA GCTGGAGATC AATTTCAATA CACTGCAGAC CAAGCTGCGG 
CTGAGCCACC GGCCCGCCTT CATGCCCTCC GAGGGCAAGC TCGTCTCGGA TATCGCCAAT 
GCATGGCGTG GGCTGGAGCA AGTAGAGAAG GGCTATGAGG ACTGGCTGCT CTCAGAGATC 
CGGCGCCTGC AGCGGCTCCA GCACCTGGCT GAGAAATTCC AGCAGAAAGC CTCCTTGCAT 
GAAGCCTGGA CCCGGGGGAA GGAGGATATG CTGAGCCAGC GCGACTACGA GTCGGCCTCC 
CTGCAGGAGG TGCGGGCGTT GCTGCGGCGC CACGAGGCCT TTGAGAGCGA CCTGGCAGCG 
CATCAGGACC GCGTGGAGCA CATCGCCGCG CTGGCCCAGG AGCTCAATGA GCTGGACTAC 
CATGAGGCAG CCTCGGTGAA TAGCCGCTGC CAGGCCATCT GCGACCAGTG GGACAATCTG 
GGCACGCTGA CCCAGAAGAG GAGGGATGCG CTAGAACGGA TGGAGAAGCT CCTGGAGACC 
ATTGACCAGC TGCAACTGGA GTTTGCTCGT CGGGCAGCGC CCTTCAACAA CTGGCTGGAT 
GGTGCCGTGG AGGACCTGCA GGACGTGTGG CTGGTGCACT CAGTGGAGGA AACCCAGAGC 
CTGCTGACTG CACATGAGCA ATTCAAGGCT ACGTTGCCAG AGGCTGACCG AGAGCGAGGA 
GCAATCCTCG GTATCCAGGG TGAGATCCAG AAGATCTGCC AAACGTATGG ACTGCGGCCC 
AGCTCCACCA ACCCCTACAT CAACCTCACA CCACAGGACA TCAACACCAA GTGGGACATG 
GTCCGAAAGC TGGTGCCCAG CCGTGACCAG ACCCTGCAGG AAGAGCTTGC CCGGCAGCAG 
CTGAATGAGA GGCTCCGGCG ACAGTTCGCA GCTCAAGCCA ATGCTGTTGG GCCCTGGATC 
CAGGGGAAGG TGGAGGAGGT GGGGCGCCTA GCCACAGGGA TGGCTGGCTC TCTGGAGGAA 
CAGATGGCTG GACTGCGGCA ACAGGAGCAG AACATCATCA ACTACAAGAG CAACATTGAC 
CGGCTGGAGG GTGACCACCA GCTGCTGCAG GAGAGCCTAG TGTTTGACAA CAAGCACACC 
GTCTACAGCA TGGAGCACAT CCGCGTAGGC TGGGAGCAGC TGCTCACCTC CATCGCCCGT 
ACCATCAATG AAGTGGAGAA CCAGGTACTG ACCCGAGATG CCAAGGGCCT GAGCCAGGAG 
CAGCTCAACG AGTTCCGGGC ATCCTTCAAC CACTTTGACC GGGTCAGCGA GGGCCCAATT 
CTGTGGGCGG TGGATGTGGG GGCTGGTGGG GCTGGAGGAC CAAGTCTGAT ATGCACTCAT 
GCACTACAGA AGCGGAACGG GATGATGGAG CCTGATGACT TCCGGGCTTG CCTCATCTCC 
ATGGGCTATG ATCTGGGAGA AGTAGAGTTT GCACGAATTA TGACCATGGT GGACCCCAAC 
GCAGCCGGCG TCGTGACCTT CCAGGCCTTC ATCGACTTCA TGACCCGAGA GACAGCTGAG 
ACTGACACAG CTGAGCAAGT TGTGGCCTCC TTCAAGATCC TGGCAGGAGA TAAGAACTAC 
ATCACTCCTG AAGAGCTGCG GCGGGAGCTC CCTGCTGAGC AGGCCGAGTA CTGCATCCGT 
CGTATGGCAC CCTACAAGGG CTCTGGGGCT CCACCTGGAG CCCTGGACTA CGTGGCCTTC 
TCCAGTGCCC TCTATGGGGA GAGTGACCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTN3 ( XM_847243.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_852336.1 Canis lupus familiaris actinin, alpha 3 (ACTN3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe20303
Clone ID Related Accession (Same CDS sequence)	XM_847243.4
Accession Version	XM_847243.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	alpha-actinin-3 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_847243.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGG TTATGCAGCC CGAGGGCCTG GGGGTCGGGG AGGGGCCCTT CGTGGGCGGC 
AGCGGGGGCG GCGAGTACAT GGAACAGGAG GAAGACTGGG ACCGCGACCT ACTGCTCGAC 
CCGGCCTGGG AGAAGCAGCA GCGGAAAACC TTCACTGCCT GGTGCAACTC ACACCTGCGC 
AAGGCTGGCA CCCAGATTGA AAACATAGAG GAGGATTTCC GCAATGGCCT CAAACTCATG 
TTGCTTTTGG AGGTCATTTC AGGAGAGAGG CTACCTAGGC CAGACAAAGG CAAGATGCGC 
TTCCACAAAA TTGCCAATGT CAACAAGGCC CTGGACTTCA TTGCCAGCAA GGGGGTTAAG 
CTGGTGTCCA TTGGTGCTGA AGAGATTGTT GACGGGAACC TGAAGATGAC CCTGGGCATG 
ATCTGGACCA TCATCCTTCG CTTTGCTATC CAGGACATCT CTGTGGAGGA AACCTCTGCC 
AAGGAAGGCT TGCTCCTGTG GTGCCAGCGG AAGACAGCTC CGTACCGCAA CGTCAACGTG 
CAAAACTTCC ACACCAGCTG GAAGGATGGT TTGGCCCTCT GTGCTCTCAT CCACCGACAC 
CGCCCCGATC TCATCGATTA TGCCAAACTA CGCAAGGATG ACCCCATTGG CAATCTAAAC 
ACGGCCTTCG AGGTTGCAGA GAAATACTTG GACATCCCTA GGATGTTGGA TGCAGAAGAC 
ATCGTGAACA CCCCAAAGCC TGATGAAAAG GCCATCATGA CCTATGTTTC CTGCTTCTAC 
CATGCCTTTG CCGGGGCTGA GCAGGCAGAG ACAGCTGCCA ACAGGATCTG CAAGGTGCTG 
GCTGTGAACC AGGAGAACGA GAAGCTGATG GAGGAGTACG AGAAGCTTGC CAGTGAGCTG 
CTGGAATGGA TCCGTCGCAC CATACCATGG CTGGAGAACC GTGTGGGCGA GCCCAGCATG 
AGTGCCATGC AGCGCAAGCT GGAGGACTTT CGGGACTACC GGCGCCTGCA TAAGCCACCC 
CGAGTGCAGG AGAAGTGCCA GCTGGAGATC AATTTCAATA CACTGCAGAC CAAGCTGCGG 
CTGAGCCACC GGCCCGCCTT CATGCCCTCC GAGGGCAAGC TCGTCTCGGA TATCGCCAAT 
GCATGGCGTG GGCTGGAGCA AGTAGAGAAG GGCTATGAGG ACTGGCTGCT CTCAGAGATC 
CGGCGCCTGC AGCGGCTCCA GCACCTGGCT GAGAAATTCC AGCAGAAAGC CTCCTTGCAT 
GAAGCCTGGA CCCGGGGGAA GGAGGATATG CTGAGCCAGC GCGACTACGA GTCGGCCTCC 
CTGCAGGAGG TGCGGGCGTT GCTGCGGCGC CACGAGGCCT TTGAGAGCGA CCTGGCAGCG 
CATCAGGACC GCGTGGAGCA CATCGCCGCG CTGGCCCAGG AGCTCAATGA GCTGGACTAC 
CATGAGGCAG CCTCGGTGAA TAGCCGCTGC CAGGCCATCT GCGACCAGTG GGACAATCTG 
GGCACGCTGA CCCAGAAGAG GAGGGATGCG CTAGAACGGA TGGAGAAGCT CCTGGAGACC 
ATTGACCAGC TGCAACTGGA GTTTGCTCGT CGGGCAGCGC CCTTCAACAA CTGGCTGGAT 
GGTGCCGTGG AGGACCTGCA GGACGTGTGG CTGGTGCACT CAGTGGAGGA AACCCAGAGC 
CTGCTGACTG CACATGAGCA ATTCAAGGCT ACGTTGCCAG AGGCTGACCG AGAGCGAGGA 
GCAATCCTCG GTATCCAGGG TGAGATCCAG AAGATCTGCC AAACGTATGG ACTGCGGCCC 
AGCTCCACCA ACCCCTACAT CAACCTCACA CCACAGGACA TCAACACCAA GTGGGACATG 
GTCCGAAAGC TGGTGCCCAG CCGTGACCAG ACCCTGCAGG AAGAGCTTGC CCGGCAGCAG 
CTGAATGAGA GGCTCCGGCG ACAGTTCGCA GCTCAAGCCA ATGCTGTTGG GCCCTGGATC 
CAGGGGAAGG TGGAGGAGGT GGGGCGCCTA GCCACAGGGA TGGCTGGCTC TCTGGAGGAA 
CAGATGGCTG GACTGCGGCA ACAGGAGCAG AACATCATCA ACTACAAGAG CAACATTGAC 
CGGCTGGAGG GTGACCACCA GCTGCTGCAG GAGAGCCTAG TGTTTGACAA CAAGCACACC 
GTCTACAGCA TGGAGCACAT CCGCGTAGGC TGGGAGCAGC TGCTCACCTC CATCGCCCGT 
ACCATCAATG AAGTGGAGAA CCAGGTACTG ACCCGAGATG CCAAGGGCCT GAGCCAGGAG 
CAGCTCAACG AGTTCCGGGC ATCCTTCAAC CACTTTGACC GGAAGCGGAA CGGGATGATG 
GAGCCTGATG ACTTCCGGGC TTGCCTCATC TCCATGGGCT ATGATCTGGG AGAAGTAGAG 
TTTGCACGAA TTATGACCAT GGTGGACCCC AACGCAGCCG GCGTCGTGAC CTTCCAGGCC 
TTCATCGACT TCATGACCCG AGAGACAGCT GAGACTGACA CAGCTGAGCA AGTTGTGGCC 
TCCTTCAAGA TCCTGGCAGG AGATAAGAAC TACATCACTC CTGAAGAGCT GCGGCGGGAG 
CTCCCTGCTG AGCAGGCCGA GTACTGCATC CGTCGTATGG CACCCTACAA GGGCTCTGGG 
GCTCCACCTG GAGCCCTGGA CTACGTGGCC TTCTCCAGTG CCCTCTATGG GGAGAGTGAC 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_852336.1
CDS	77..2782
Translation	MMMVMQPEGLGVGEGPFVGGSGGGEYMEQEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRNGLKLMLLLEVISGERLPRPDKGKMRFHKIANVNKALDFIASKGVKLVSIGAEEIVDGNLKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYRNVNVQNFHTSWKDGLALCALIHRHRPDLIDYAKLRKDDPIGNLNTAFEVAEKYLDIPRMLDAEDIVNTPKPDEKAIMTYVSCFYHAFAGAEQAETAANRICKVLAVNQENEKLMEEYEKLASELLEWIRRTIPWLENRVGEPSMSAMQRKLEDFRDYRRLHKPPRVQEKCQLEINFNTLQTKLRLSHRPAFMPSEGKLVSDIANAWRGLEQVEKGYEDWLLSEIRRLQRLQHLAEKFQQKASLHEAWTRGKEDMLSQRDYESASLQEVRALLRRHEAFESDLAAHQDRVEHIAALAQELNELDYHEAASVNSRCQAICDQWDNLGTLTQKRRDALERMEKLLETIDQLQLEFARRAAPFNNWLDGAVEDLQDVWLVHSVEETQSLLTAHEQFKATLPEADRERGAILGIQGEIQKICQTYGLRPSSTNPYINLTPQDINTKWDMVRKLVPSRDQTLQEELARQQLNERLRRQFAAQANAVGPWIQGKVEEVGRLATGMAGSLEEQMAGLRQQEQNIINYKSNIDRLEGDHQLLQESLVFDNKHTVYSMEHIRVGWEQLLTSIARTINEVENQVLTRDAKGLSQEQLNEFRASFNHFDRKRNGMMEPDDFRACLISMGYDLGEVEFARIMTMVDPNAAGVVTFQAFIDFMTRETAETDTAEQVVASFKILAGDKNYITPEELRRELPAEQAEYCIRRMAPYKGSGAPPGALDYVAFSSALYGESDL

Target ORF information:

RefSeq Version	XM_847243.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris actinin, alpha 3 (ACTN3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_847243.4

ORF Insert Sequence:

ATGATGATGG TTATGCAGCC CGAGGGCCTG GGGGTCGGGG AGGGGCCCTT CGTGGGCGGC 
AGCGGGGGCG GCGAGTACAT GGAACAGGAG GAAGACTGGG ACCGCGACCT ACTGCTCGAC 
CCGGCCTGGG AGAAGCAGCA GCGGAAAACC TTCACTGCCT GGTGCAACTC ACACCTGCGC 
AAGGCTGGCA CCCAGATTGA AAACATAGAG GAGGATTTCC GCAATGGCCT CAAACTCATG 
TTGCTTTTGG AGGTCATTTC AGGAGAGAGG CTACCTAGGC CAGACAAAGG CAAGATGCGC 
TTCCACAAAA TTGCCAATGT CAACAAGGCC CTGGACTTCA TTGCCAGCAA GGGGGTTAAG 
CTGGTGTCCA TTGGTGCTGA AGAGATTGTT GACGGGAACC TGAAGATGAC CCTGGGCATG 
ATCTGGACCA TCATCCTTCG CTTTGCTATC CAGGACATCT CTGTGGAGGA AACCTCTGCC 
AAGGAAGGCT TGCTCCTGTG GTGCCAGCGG AAGACAGCTC CGTACCGCAA CGTCAACGTG 
CAAAACTTCC ACACCAGCTG GAAGGATGGT TTGGCCCTCT GTGCTCTCAT CCACCGACAC 
CGCCCCGATC TCATCGATTA TGCCAAACTA CGCAAGGATG ACCCCATTGG CAATCTAAAC 
ACGGCCTTCG AGGTTGCAGA GAAATACTTG GACATCCCTA GGATGTTGGA TGCAGAAGAC 
ATCGTGAACA CCCCAAAGCC TGATGAAAAG GCCATCATGA CCTATGTTTC CTGCTTCTAC 
CATGCCTTTG CCGGGGCTGA GCAGGCAGAG ACAGCTGCCA ACAGGATCTG CAAGGTGCTG 
GCTGTGAACC AGGAGAACGA GAAGCTGATG GAGGAGTACG AGAAGCTTGC CAGTGAGCTG 
CTGGAATGGA TCCGTCGCAC CATACCATGG CTGGAGAACC GTGTGGGCGA GCCCAGCATG 
AGTGCCATGC AGCGCAAGCT GGAGGACTTT CGGGACTACC GGCGCCTGCA TAAGCCACCC 
CGAGTGCAGG AGAAGTGCCA GCTGGAGATC AATTTCAATA CACTGCAGAC CAAGCTGCGG 
CTGAGCCACC GGCCCGCCTT CATGCCCTCC GAGGGCAAGC TCGTCTCGGA TATCGCCAAT 
GCATGGCGTG GGCTGGAGCA AGTAGAGAAG GGCTATGAGG ACTGGCTGCT CTCAGAGATC 
CGGCGCCTGC AGCGGCTCCA GCACCTGGCT GAGAAATTCC AGCAGAAAGC CTCCTTGCAT 
GAAGCCTGGA CCCGGGGGAA GGAGGATATG CTGAGCCAGC GCGACTACGA GTCGGCCTCC 
CTGCAGGAGG TGCGGGCGTT GCTGCGGCGC CACGAGGCCT TTGAGAGCGA CCTGGCAGCG 
CATCAGGACC GCGTGGAGCA CATCGCCGCG CTGGCCCAGG AGCTCAATGA GCTGGACTAC 
CATGAGGCAG CCTCGGTGAA TAGCCGCTGC CAGGCCATCT GCGACCAGTG GGACAATCTG 
GGCACGCTGA CCCAGAAGAG GAGGGATGCG CTAGAACGGA TGGAGAAGCT CCTGGAGACC 
ATTGACCAGC TGCAACTGGA GTTTGCTCGT CGGGCAGCGC CCTTCAACAA CTGGCTGGAT 
GGTGCCGTGG AGGACCTGCA GGACGTGTGG CTGGTGCACT CAGTGGAGGA AACCCAGAGC 
CTGCTGACTG CACATGAGCA ATTCAAGGCT ACGTTGCCAG AGGCTGACCG AGAGCGAGGA 
GCAATCCTCG GTATCCAGGG TGAGATCCAG AAGATCTGCC AAACGTATGG ACTGCGGCCC 
AGCTCCACCA ACCCCTACAT CAACCTCACA CCACAGGACA TCAACACCAA GTGGGACATG 
GTCCGAAAGC TGGTGCCCAG CCGTGACCAG ACCCTGCAGG AAGAGCTTGC CCGGCAGCAG 
CTGAATGAGA GGCTCCGGCG ACAGTTCGCA GCTCAAGCCA ATGCTGTTGG GCCCTGGATC 
CAGGGGAAGG TGGAGGAGGT GGGGCGCCTA GCCACAGGGA TGGCTGGCTC TCTGGAGGAA 
CAGATGGCTG GACTGCGGCA ACAGGAGCAG AACATCATCA ACTACAAGAG CAACATTGAC 
CGGCTGGAGG GTGACCACCA GCTGCTGCAG GAGAGCCTAG TGTTTGACAA CAAGCACACC 
GTCTACAGCA TGGAGCACAT CCGCGTAGGC TGGGAGCAGC TGCTCACCTC CATCGCCCGT 
ACCATCAATG AAGTGGAGAA CCAGGTACTG ACCCGAGATG CCAAGGGCCT GAGCCAGGAG 
CAGCTCAACG AGTTCCGGGC ATCCTTCAAC CACTTTGACC GGAAGCGGAA CGGGATGATG 
GAGCCTGATG ACTTCCGGGC TTGCCTCATC TCCATGGGCT ATGATCTGGG AGAAGTAGAG 
TTTGCACGAA TTATGACCAT GGTGGACCCC AACGCAGCCG GCGTCGTGAC CTTCCAGGCC 
TTCATCGACT TCATGACCCG AGAGACAGCT GAGACTGACA CAGCTGAGCA AGTTGTGGCC 
TCCTTCAAGA TCCTGGCAGG AGATAAGAAC TACATCACTC CTGAAGAGCT GCGGCGGGAG 
CTCCCTGCTG AGCAGGCCGA GTACTGCATC CGTCGTATGG CACCCTACAA GGGCTCTGGG 
GCTCCACCTG GAGCCCTGGA CTACGTGGCC TTCTCCAGTG CCCTCTATGG GGAGAGTGAC 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTN3 ( XM_022405619.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022261327.1 Canis lupus familiaris actinin alpha 3 (ACTN3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe294888
Clone ID Related Accession (Same CDS sequence)	XM_022405619.1
Accession Version	XM_022405619.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2637bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	alpha-actinin-3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCTG GCCAGTTAAT TGTACAGATG GGGAGACTGA GCACAAGGAA GGGCAAAGGG 
TATGCTCCAG GTCACAGAAC CTTCACTGCC TGGTGCAACT CACACCTGCG CAAGGCTGGC 
ACCCAGATTG AAAACATAGA GGAGGATTTC CGCAATGGCC TCAAACTCAT GTTGCTTTTG 
GAGGTCATTT CAGGAGAGAG GCTACCTAGG CCAGACAAAG GCAAGATGCG CTTCCACAAA 
ATTGCCAATG TCAACAAGGC CCTGGACTTC ATTGCCAGCA AGGGGGTTAA GCTGGTGTCC 
ATTGGTGCTG AAGAGATTGT TGACGGGAAC CTGAAGATGA CCCTGGGCAT GATCTGGACC 
ATCATCCTTC GCTTTGCTAT CCAGGACATC TCTGTGGAGG AAACCTCTGC CAAGGAAGGC 
TTGCTCCTGT GGTGCCAGCG GAAGACAGCT CCGTACCGCA ACGTCAACGT GCAAAACTTC 
CACACCAGCT GGAAGGATGG TTTGGCCCTC TGTGCTCTCA TCCACCGACA CCGCCCCGAT 
CTCATCGATT ATGCCAAACT ACGCAAGGAT GACCCCATTG GCAATCTAAA CACGGCCTTC 
GAGGTTGCAG AGAAATACTT GGACATCCCT AGGATGTTGG ATGCAGAAGA CATCGTGAAC 
ACCCCAAAGC CTGATGAAAA GGCCATCATG ACCTATGTTT CCTGCTTCTA CCATGCCTTT 
GCCGGGGCTG AGCAGGCAGA GACAGCTGCC AACAGGATCT GCAAGGTGCT GGCTGTGAAC 
CAGGAGAACG AGAAGCTGAT GGAGGAGTAC GAGAAGCTTG CCAGTGAGCT GCTGGAATGG 
ATCCGTCGCA CCATACCATG GCTGGAGAAC CGTGTGGGCG AGCCCAGCAT GAGTGCCATG 
CAGCGCAAGC TGGAGGACTT TCGGGACTAC CGGCGCCTGC ATAAGCCACC CCGAGTGCAG 
GAGAAGTGCC AGCTGGAGAT CAATTTCAAT ACACTGCAGA CCAAGCTGCG GCTGAGCCAC 
CGGCCCGCCT TCATGCCCTC CGAGGGCAAG CTCGTCTCGG ATATCGCCAA TGCATGGCGT 
GGGCTGGAGC AAGTAGAGAA GGGCTATGAG GACTGGCTGC TCTCAGAGAT CCGGCGCCTG 
CAGCGGCTCC AGCACCTGGC TGAGAAATTC CAGCAGAAAG CCTCCTTGCA TGAAGCCTGG 
ACCCGGGGGA AGGAGGATAT GCTGAGCCAG CGCGACTACG AGTCGGCCTC CCTGCAGGAG 
GTGCGGGCGT TGCTGCGGCG CCACGAGGCC TTTGAGAGCG ACCTGGCAGC GCATCAGGAC 
CGCGTGGAGC ACATCGCCGC GCTGGCCCAG GAGCTCAATG AGCTGGACTA CCATGAGGCA 
GCCTCGGTGA ATAGCCGCTG CCAGGCCATC TGCGACCAGT GGGACAATCT GGGCACGCTG 
ACCCAGAAGA GGAGGGATGC GCTAGAACGG ATGGAGAAGC TCCTGGAGAC CATTGACCAG 
CTGCAACTGG AGTTTGCTCG TCGGGCAGCG CCCTTCAACA ACTGGCTGGA TGGTGCCGTG 
GAGGACCTGC AGGACGTGTG GCTGGTGCAC TCAGTGGAGG AAACCCAGAG CCTGCTGACT 
GCACATGAGC AATTCAAGGC TACGTTGCCA GAGGCTGACC GAGAGCGAGG AGCAATCCTC 
GGTATCCAGG GTGAGATCCA GAAGATCTGC CAAACGTATG GACTGCGGCC CAGCTCCACC 
AACCCCTACA TCAACCTCAC ACCACAGGAC ATCAACACCA AGTGGGACAT GGTCCGAAAG 
CTGGTGCCCA GCCGTGACCA GACCCTGCAG GAAGAGCTTG CCCGGCAGCA GCTGAATGAG 
AGGCTCCGGC GACAGTTCGC AGCTCAAGCC AATGCTGTTG GGCCCTGGAT CCAGGGGAAG 
GTGGAGGAGG TGGGGCGCCT AGCCACAGGG ATGGCTGGCT CTCTGGAGGA ACAGATGGCT 
GGACTGCGGC AACAGGAGCA GAACATCATC AACTACAAGA GCAACATTGA CCGGCTGGAG 
GGTGACCACC AGCTGCTGCA GGAGAGCCTA GTGTTTGACA ACAAGCACAC CGTCTACAGC 
ATGGAGCACA TCCGCGTAGG CTGGGAGCAG CTGCTCACCT CCATCGCCCG TACCATCAAT 
GAAGTGGAGA ACCAGGTACT GACCCGAGAT GCCAAGGGCC TGAGCCAGGA GCAGCTCAAC 
GAGTTCCGGG CATCCTTCAA CCACTTTGAC CGGAAGCGGA ACGGGATGAT GGAGCCTGAT 
GACTTCCGGG CTTGCCTCAT CTCCATGGGC TATGATCTGG GAGAAGTAGA GTTTGCACGA 
ATTATGACCA TGGTGGACCC CAACGCAGCC GGCGTCGTGA CCTTCCAGGC CTTCATCGAC 
TTCATGACCC GAGAGACAGC TGAGACTGAC ACAGCTGAGC AAGTTGTGGC CTCCTTCAAG 
ATCCTGGCAG GAGATAAGAA CTACATCACT CCTGAAGAGC TGCGGCGGGA GCTCCCTGCT 
GAGCAGGCCG AGTACTGCAT CCGTCGTATG GCACCCTACA AGGGCTCTGG GGCTCCACCT 
GGAGCCCTGG ACTACGTGGC CTTCTCCAGT GCCCTCTATG GGGAGAGTGA CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022261327.1
CDS	16..2652
Translation	MKSGQLIVQMGRLSTRKGKGYAPGHRTFTAWCNSHLRKAGTQIENIEEDFRNGLKLMLLLEVISGERLPRPDKGKMRFHKIANVNKALDFIASKGVKLVSIGAEEIVDGNLKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYRNVNVQNFHTSWKDGLALCALIHRHRPDLIDYAKLRKDDPIGNLNTAFEVAEKYLDIPRMLDAEDIVNTPKPDEKAIMTYVSCFYHAFAGAEQAETAANRICKVLAVNQENEKLMEEYEKLASELLEWIRRTIPWLENRVGEPSMSAMQRKLEDFRDYRRLHKPPRVQEKCQLEINFNTLQTKLRLSHRPAFMPSEGKLVSDIANAWRGLEQVEKGYEDWLLSEIRRLQRLQHLAEKFQQKASLHEAWTRGKEDMLSQRDYESASLQEVRALLRRHEAFESDLAAHQDRVEHIAALAQELNELDYHEAASVNSRCQAICDQWDNLGTLTQKRRDALERMEKLLETIDQLQLEFARRAAPFNNWLDGAVEDLQDVWLVHSVEETQSLLTAHEQFKATLPEADRERGAILGIQGEIQKICQTYGLRPSSTNPYINLTPQDINTKWDMVRKLVPSRDQTLQEELARQQLNERLRRQFAAQANAVGPWIQGKVEEVGRLATGMAGSLEEQMAGLRQQEQNIINYKSNIDRLEGDHQLLQESLVFDNKHTVYSMEHIRVGWEQLLTSIARTINEVENQVLTRDAKGLSQEQLNEFRASFNHFDRKRNGMMEPDDFRACLISMGYDLGEVEFARIMTMVDPNAAGVVTFQAFIDFMTRETAETDTAEQVVASFKILAGDKNYITPEELRRELPAEQAEYCIRRMAPYKGSGAPPGALDYVAFSSALYGESDL

Target ORF information:

RefSeq Version	XM_022405619.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris actinin alpha 3 (ACTN3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022405619.1

ORF Insert Sequence:

ATGAAGTCTG GCCAGTTAAT TGTACAGATG GGGAGACTGA GCACAAGGAA GGGCAAAGGG 
TATGCTCCAG GTCACAGAAC CTTCACTGCC TGGTGCAACT CACACCTGCG CAAGGCTGGC 
ACCCAGATTG AAAACATAGA GGAGGATTTC CGCAATGGCC TCAAACTCAT GTTGCTTTTG 
GAGGTCATTT CAGGAGAGAG GCTACCTAGG CCAGACAAAG GCAAGATGCG CTTCCACAAA 
ATTGCCAATG TCAACAAGGC CCTGGACTTC ATTGCCAGCA AGGGGGTTAA GCTGGTGTCC 
ATTGGTGCTG AAGAGATTGT TGACGGGAAC CTGAAGATGA CCCTGGGCAT GATCTGGACC 
ATCATCCTTC GCTTTGCTAT CCAGGACATC TCTGTGGAGG AAACCTCTGC CAAGGAAGGC 
TTGCTCCTGT GGTGCCAGCG GAAGACAGCT CCGTACCGCA ACGTCAACGT GCAAAACTTC 
CACACCAGCT GGAAGGATGG TTTGGCCCTC TGTGCTCTCA TCCACCGACA CCGCCCCGAT 
CTCATCGATT ATGCCAAACT ACGCAAGGAT GACCCCATTG GCAATCTAAA CACGGCCTTC 
GAGGTTGCAG AGAAATACTT GGACATCCCT AGGATGTTGG ATGCAGAAGA CATCGTGAAC 
ACCCCAAAGC CTGATGAAAA GGCCATCATG ACCTATGTTT CCTGCTTCTA CCATGCCTTT 
GCCGGGGCTG AGCAGGCAGA GACAGCTGCC AACAGGATCT GCAAGGTGCT GGCTGTGAAC 
CAGGAGAACG AGAAGCTGAT GGAGGAGTAC GAGAAGCTTG CCAGTGAGCT GCTGGAATGG 
ATCCGTCGCA CCATACCATG GCTGGAGAAC CGTGTGGGCG AGCCCAGCAT GAGTGCCATG 
CAGCGCAAGC TGGAGGACTT TCGGGACTAC CGGCGCCTGC ATAAGCCACC CCGAGTGCAG 
GAGAAGTGCC AGCTGGAGAT CAATTTCAAT ACACTGCAGA CCAAGCTGCG GCTGAGCCAC 
CGGCCCGCCT TCATGCCCTC CGAGGGCAAG CTCGTCTCGG ATATCGCCAA TGCATGGCGT 
GGGCTGGAGC AAGTAGAGAA GGGCTATGAG GACTGGCTGC TCTCAGAGAT CCGGCGCCTG 
CAGCGGCTCC AGCACCTGGC TGAGAAATTC CAGCAGAAAG CCTCCTTGCA TGAAGCCTGG 
ACCCGGGGGA AGGAGGATAT GCTGAGCCAG CGCGACTACG AGTCGGCCTC CCTGCAGGAG 
GTGCGGGCGT TGCTGCGGCG CCACGAGGCC TTTGAGAGCG ACCTGGCAGC GCATCAGGAC 
CGCGTGGAGC ACATCGCCGC GCTGGCCCAG GAGCTCAATG AGCTGGACTA CCATGAGGCA 
GCCTCGGTGA ATAGCCGCTG CCAGGCCATC TGCGACCAGT GGGACAATCT GGGCACGCTG 
ACCCAGAAGA GGAGGGATGC GCTAGAACGG ATGGAGAAGC TCCTGGAGAC CATTGACCAG 
CTGCAACTGG AGTTTGCTCG TCGGGCAGCG CCCTTCAACA ACTGGCTGGA TGGTGCCGTG 
GAGGACCTGC AGGACGTGTG GCTGGTGCAC TCAGTGGAGG AAACCCAGAG CCTGCTGACT 
GCACATGAGC AATTCAAGGC TACGTTGCCA GAGGCTGACC GAGAGCGAGG AGCAATCCTC 
GGTATCCAGG GTGAGATCCA GAAGATCTGC CAAACGTATG GACTGCGGCC CAGCTCCACC 
AACCCCTACA TCAACCTCAC ACCACAGGAC ATCAACACCA AGTGGGACAT GGTCCGAAAG 
CTGGTGCCCA GCCGTGACCA GACCCTGCAG GAAGAGCTTG CCCGGCAGCA GCTGAATGAG 
AGGCTCCGGC GACAGTTCGC AGCTCAAGCC AATGCTGTTG GGCCCTGGAT CCAGGGGAAG 
GTGGAGGAGG TGGGGCGCCT AGCCACAGGG ATGGCTGGCT CTCTGGAGGA ACAGATGGCT 
GGACTGCGGC AACAGGAGCA GAACATCATC AACTACAAGA GCAACATTGA CCGGCTGGAG 
GGTGACCACC AGCTGCTGCA GGAGAGCCTA GTGTTTGACA ACAAGCACAC CGTCTACAGC 
ATGGAGCACA TCCGCGTAGG CTGGGAGCAG CTGCTCACCT CCATCGCCCG TACCATCAAT 
GAAGTGGAGA ACCAGGTACT GACCCGAGAT GCCAAGGGCC TGAGCCAGGA GCAGCTCAAC 
GAGTTCCGGG CATCCTTCAA CCACTTTGAC CGGAAGCGGA ACGGGATGAT GGAGCCTGAT 
GACTTCCGGG CTTGCCTCAT CTCCATGGGC TATGATCTGG GAGAAGTAGA GTTTGCACGA 
ATTATGACCA TGGTGGACCC CAACGCAGCC GGCGTCGTGA CCTTCCAGGC CTTCATCGAC 
TTCATGACCC GAGAGACAGC TGAGACTGAC ACAGCTGAGC AAGTTGTGGC CTCCTTCAAG 
ATCCTGGCAG GAGATAAGAA CTACATCACT CCTGAAGAGC TGCGGCGGGA GCTCCCTGCT 
GAGCAGGCCG AGTACTGCAT CCGTCGTATG GCACCCTACA AGGGCTCTGG GGCTCCACCT 
GGAGCCCTGG ACTACGTGGC CTTCTCCAGT GCCCTCTATG GGGAGAGTGA CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTN3 ( XM_005631429.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005631486.2 Canis lupus familiaris actinin alpha 3 (ACTN3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe294886
Clone ID Related Accession (Same CDS sequence)	XM_005631429.3
Accession Version	XM_005631429.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	alpha-actinin-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005631429.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGATAG GGCTCTGTTT CCCACCAGCG CGACGCAGGC AGAGGGTCAG GAGGAGGCCT 
GAGAGGCAGC TTGTAGCGGT CAACAGTCAC TATTATTATC GTCATCTCCG TTGTACTCAT 
GAGGAAACAG AGAGGGGCCG TGGCTTTCCC ATGGTCACAC AGCACCCAAG AGGCGGAGCC 
GGAACGGGAA CCCCGAATCT CCGCTATGGG ATCCACCCCG GCGCCCCCAA ATCCTTCAGT 
CCCCTGGTCT CAGGGCAACC TCTTGCCTGG TCCCTACCCA AACGCCCTCG CCCCCATCCG 
GCCCCACCCA CCTCCCCCAT ATTTAGCGCG AATCCGATCC GGGGCTGGGC CGGGCGCTAC 
TTAACGCGGC CCGGGTGTTC TGGAGAGCGC GGAGCGGAGC GAGCCAGGAG CCCCAGCAAG 
ATGATGATGG TTATGCAGCC CGAGGGCCTG GGGGTCGGGG AGGGGCCCTT CGTGGGCGGC 
AGCGGGGGCG GCGAGTACAT GGAACAGGAG GAAGACTGGG ACCGCGACCT ACTGCTCGAC 
CCGGCCTGGG AGAAGCAGCA GCGGAAAACC TTCACTGCCT GGTGCAACTC ACACCTGCGC 
AAGGCTGGCA CCCAGATTGA AAACATAGAG GAGGATTTCC GCAATGGCCT CAAACTCATG 
TTGCTTTTGG AGGTCATTTC AGGAGAGAGG CTACCTAGGC CAGACAAAGG CAAGATGCGC 
TTCCACAAAA TTGCCAATGT CAACAAGGCC CTGGACTTCA TTGCCAGCAA GGGGGTTAAG 
CTGGTGTCCA TTGGTGCTGA AGAGATTGTT GACGGGAACC TGAAGATGAC CCTGGGCATG 
ATCTGGACCA TCATCCTTCG CTTTGCTATC CAGGACATCT CTGTGGAGGA AACCTCTGCC 
AAGGAAGGCT TGCTCCTGTG GTGCCAGCGG AAGACAGCTC CGTACCGCAA CGTCAACGTG 
CAAAACTTCC ACACCAGCTG GAAGGATGGT TTGGCCCTCT GTGCTCTCAT CCACCGACAC 
CGCCCCGATC TCATCGATTA TGCCAAACTA CGCAAGGATG ACCCCATTGG CAATCTAAAC 
ACGGCCTTCG AGGTTGCAGA GAAATACTTG GACATCCCTA GGATGTTGGA TGCAGAAGAC 
ATCGTGAACA CCCCAAAGCC TGATGAAAAG GCCATCATGA CCTATGTTTC CTGCTTCTAC 
CATGCCTTTG CCGGGGCTGA GCAGGCAGAG ACAGCTGCCA ACAGGATCTG CAAGGTGCTG 
GCTGTGAACC AGGAGAACGA GAAGCTGATG GAGGAGTACG AGAAGCTTGC CAGTGAGCTG 
CTGGAATGGA TCCGTCGCAC CATACCATGG CTGGAGAACC GTGTGGGCGA GCCCAGCATG 
AGTGCCATGC AGCGCAAGCT GGAGGACTTT CGGGACTACC GGCGCCTGCA TAAGCCACCC 
CGAGTGCAGG AGAAGTGCCA GCTGGAGATC AATTTCAATA CACTGCAGAC CAAGCTGCGG 
CTGAGCCACC GGCCCGCCTT CATGCCCTCC GAGGGCAAGC TCGTCTCGGA TATCGCCAAT 
GCATGGCGTG GGCTGGAGCA AGTAGAGAAG GGCTATGAGG ACTGGCTGCT CTCAGAGATC 
CGGCGCCTGC AGCGGCTCCA GCACCTGGCT GAGAAATTCC AGCAGAAAGC CTCCTTGCAT 
GAAGCCTGGA CCCGGGGGAA GGAGGATATG CTGAGCCAGC GCGACTACGA GTCGGCCTCC 
CTGCAGGAGG TGCGGGCGTT GCTGCGGCGC CACGAGGCCT TTGAGAGCGA CCTGGCAGCG 
CATCAGGACC GCGTGGAGCA CATCGCCGCG CTGGCCCAGG AGCTCAATGA GCTGGACTAC 
CATGAGGCAG CCTCGGTGAA TAGCCGCTGC CAGGCCATCT GCGACCAGTG GGACAATCTG 
GGCACGCTGA CCCAGAAGAG GAGGGATGCG CTAGAACGGA TGGAGAAGCT CCTGGAGACC 
ATTGACCAGC TGCAACTGGA GTTTGCTCGT CGGGCAGCGC CCTTCAACAA CTGGCTGGAT 
GGTGCCGTGG AGGACCTGCA GGACGTGTGG CTGGTGCACT CAGTGGAGGA AACCCAGAGC 
CTGCTGACTG CACATGAGCA ATTCAAGGCT ACGTTGCCAG AGGCTGACCG AGAGCGAGGA 
GCAATCCTCG GTATCCAGGG TGAGATCCAG AAGATCTGCC AAACGTATGG ACTGCGGCCC 
AGCTCCACCA ACCCCTACAT CAACCTCACA CCACAGGACA TCAACACCAA GTGGGACATG 
GTCCGAAAGC TGGTGCCCAG CCGTGACCAG ACCCTGCAGG AAGAGCTTGC CCGGCAGCAG 
CTGAATGAGA GGCTCCGGCG ACAGTTCGCA GCTCAAGCCA ATGCTGTTGG GCCCTGGATC 
CAGGGGAAGG TGGAGGAGGT GGGGCGCCTA GCCACAGGGA TGGCTGGCTC TCTGGAGGAA 
CAGATGGCTG GACTGCGGCA ACAGGAGCAG AACATCATCA ACTACAAGAG CAACATTGAC 
CGGCTGGAGG GTGACCACCA GCTGCTGCAG GAGAGCCTAG TGTTTGACAA CAAGCACACC 
GTCTACAGCA TGGAGCACAT CCGCGTAGGC TGGGAGCAGC TGCTCACCTC CATCGCCCGT 
ACCATCAATG AAGTGGAGAA CCAGGTACTG ACCCGAGATG CCAAGGGCCT GAGCCAGGAG 
CAGCTCAACG AGTTCCGGGC ATCCTTCAAC CACTTTGACC GGGTCAGCGA GGGCCCAATT 
CTGTGGGCGG TGGATGTGGG GGCTGGTGGG GCTGGAGGAC CAAGTCTGAT ATGCACTCAT 
GCACTACAGA AGCGGAACGG GATGATGGAG CCTGATGACT TCCGGGCTTG CCTCATCTCC 
ATGGGCTATG ATCTGGGAGA AGTAGAGTTT GCACGAATTA TGACCATGGT GGACCCCAAC 
GCAGCCGGCG TCGTGACCTT CCAGGCCTTC ATCGACTTCA TGACCCGAGA GACAGCTGAG 
ACTGACACAG CTGAGCAAGT TGTGGCCTCC TTCAAGATCC TGGCAGGAGA TAAGAACTAC 
ATCACTCCTG AAGAGCTGCG GCGGGAGCTC CCTGCTGAGC AGGCCGAGTA CTGCATCCGT 
CGTATGGCAC CCTACAAGGG CTCTGGGGCT CCACCTGGAG CCCTGGACTA CGTGGCCTTC 
TCCAGTGCCC TCTATGGGGA GAGTGACCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005631486.2
CDS	192..3404
Translation	MGIGLCFPPARRRQRVRRRPERQLVAVNSHYYYRHLRCTHEETERGRGFPMVTQHPRGGAGTGTPNLRYGIHPGAPKSFSPLVSGQPLAWSLPKRPRPHPAPPTSPIFSANPIRGWAGRYLTRPGCSGERGAERARSPSKMMMVMQPEGLGVGEGPFVGGSGGGEYMEQEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRNGLKLMLLLEVISGERLPRPDKGKMRFHKIANVNKALDFIASKGVKLVSIGAEEIVDGNLKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYRNVNVQNFHTSWKDGLALCALIHRHRPDLIDYAKLRKDDPIGNLNTAFEVAEKYLDIPRMLDAEDIVNTPKPDEKAIMTYVSCFYHAFAGAEQAETAANRICKVLAVNQENEKLMEEYEKLASELLEWIRRTIPWLENRVGEPSMSAMQRKLEDFRDYRRLHKPPRVQEKCQLEINFNTLQTKLRLSHRPAFMPSEGKLVSDIANAWRGLEQVEKGYEDWLLSEIRRLQRLQHLAEKFQQKASLHEAWTRGKEDMLSQRDYESASLQEVRALLRRHEAFESDLAAHQDRVEHIAALAQELNELDYHEAASVNSRCQAICDQWDNLGTLTQKRRDALERMEKLLETIDQLQLEFARRAAPFNNWLDGAVEDLQDVWLVHSVEETQSLLTAHEQFKATLPEADRERGAILGIQGEIQKICQTYGLRPSSTNPYINLTPQDINTKWDMVRKLVPSRDQTLQEELARQQLNERLRRQFAAQANAVGPWIQGKVEEVGRLATGMAGSLEEQMAGLRQQEQNIINYKSNIDRLEGDHQLLQESLVFDNKHTVYSMEHIRVGWEQLLTSIARTINEVENQVLTRDAKGLSQEQLNEFRASFNHFDRVSEGPILWAVDVGAGGAGGPSLICTHALQKRNGMMEPDDFRACLISMGYDLGEVEFARIMTMVDPNAAGVVTFQAFIDFMTRETAETDTAEQVVASFKILAGDKNYITPEELRRELPAEQAEYCIRRMAPYKGSGAPPGALDYVAFSSALYGESDL

Target ORF information:

RefSeq Version	XM_005631429.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris actinin alpha 3 (ACTN3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005631429.3

ORF Insert Sequence:

ATGGGGATAG GGCTCTGTTT CCCACCAGCG CGACGCAGGC AGAGGGTCAG GAGGAGGCCT 
GAGAGGCAGC TTGTAGCGGT CAACAGTCAC TATTATTATC GTCATCTCCG TTGTACTCAT 
GAGGAAACAG AGAGGGGCCG TGGCTTTCCC ATGGTCACAC AGCACCCAAG AGGCGGAGCC 
GGAACGGGAA CCCCGAATCT CCGCTATGGG ATCCACCCCG GCGCCCCCAA ATCCTTCAGT 
CCCCTGGTCT CAGGGCAACC TCTTGCCTGG TCCCTACCCA AACGCCCTCG CCCCCATCCG 
GCCCCACCCA CCTCCCCCAT ATTTAGCGCG AATCCGATCC GGGGCTGGGC CGGGCGCTAC 
TTAACGCGGC CCGGGTGTTC TGGAGAGCGC GGAGCGGAGC GAGCCAGGAG CCCCAGCAAG 
ATGATGATGG TTATGCAGCC CGAGGGCCTG GGGGTCGGGG AGGGGCCCTT CGTGGGCGGC 
AGCGGGGGCG GCGAGTACAT GGAACAGGAG GAAGACTGGG ACCGCGACCT ACTGCTCGAC 
CCGGCCTGGG AGAAGCAGCA GCGGAAAACC TTCACTGCCT GGTGCAACTC ACACCTGCGC 
AAGGCTGGCA CCCAGATTGA AAACATAGAG GAGGATTTCC GCAATGGCCT CAAACTCATG 
TTGCTTTTGG AGGTCATTTC AGGAGAGAGG CTACCTAGGC CAGACAAAGG CAAGATGCGC 
TTCCACAAAA TTGCCAATGT CAACAAGGCC CTGGACTTCA TTGCCAGCAA GGGGGTTAAG 
CTGGTGTCCA TTGGTGCTGA AGAGATTGTT GACGGGAACC TGAAGATGAC CCTGGGCATG 
ATCTGGACCA TCATCCTTCG CTTTGCTATC CAGGACATCT CTGTGGAGGA AACCTCTGCC 
AAGGAAGGCT TGCTCCTGTG GTGCCAGCGG AAGACAGCTC CGTACCGCAA CGTCAACGTG 
CAAAACTTCC ACACCAGCTG GAAGGATGGT TTGGCCCTCT GTGCTCTCAT CCACCGACAC 
CGCCCCGATC TCATCGATTA TGCCAAACTA CGCAAGGATG ACCCCATTGG CAATCTAAAC 
ACGGCCTTCG AGGTTGCAGA GAAATACTTG GACATCCCTA GGATGTTGGA TGCAGAAGAC 
ATCGTGAACA CCCCAAAGCC TGATGAAAAG GCCATCATGA CCTATGTTTC CTGCTTCTAC 
CATGCCTTTG CCGGGGCTGA GCAGGCAGAG ACAGCTGCCA ACAGGATCTG CAAGGTGCTG 
GCTGTGAACC AGGAGAACGA GAAGCTGATG GAGGAGTACG AGAAGCTTGC CAGTGAGCTG 
CTGGAATGGA TCCGTCGCAC CATACCATGG CTGGAGAACC GTGTGGGCGA GCCCAGCATG 
AGTGCCATGC AGCGCAAGCT GGAGGACTTT CGGGACTACC GGCGCCTGCA TAAGCCACCC 
CGAGTGCAGG AGAAGTGCCA GCTGGAGATC AATTTCAATA CACTGCAGAC CAAGCTGCGG 
CTGAGCCACC GGCCCGCCTT CATGCCCTCC GAGGGCAAGC TCGTCTCGGA TATCGCCAAT 
GCATGGCGTG GGCTGGAGCA AGTAGAGAAG GGCTATGAGG ACTGGCTGCT CTCAGAGATC 
CGGCGCCTGC AGCGGCTCCA GCACCTGGCT GAGAAATTCC AGCAGAAAGC CTCCTTGCAT 
GAAGCCTGGA CCCGGGGGAA GGAGGATATG CTGAGCCAGC GCGACTACGA GTCGGCCTCC 
CTGCAGGAGG TGCGGGCGTT GCTGCGGCGC CACGAGGCCT TTGAGAGCGA CCTGGCAGCG 
CATCAGGACC GCGTGGAGCA CATCGCCGCG CTGGCCCAGG AGCTCAATGA GCTGGACTAC 
CATGAGGCAG CCTCGGTGAA TAGCCGCTGC CAGGCCATCT GCGACCAGTG GGACAATCTG 
GGCACGCTGA CCCAGAAGAG GAGGGATGCG CTAGAACGGA TGGAGAAGCT CCTGGAGACC 
ATTGACCAGC TGCAACTGGA GTTTGCTCGT CGGGCAGCGC CCTTCAACAA CTGGCTGGAT 
GGTGCCGTGG AGGACCTGCA GGACGTGTGG CTGGTGCACT CAGTGGAGGA AACCCAGAGC 
CTGCTGACTG CACATGAGCA ATTCAAGGCT ACGTTGCCAG AGGCTGACCG AGAGCGAGGA 
GCAATCCTCG GTATCCAGGG TGAGATCCAG AAGATCTGCC AAACGTATGG ACTGCGGCCC 
AGCTCCACCA ACCCCTACAT CAACCTCACA CCACAGGACA TCAACACCAA GTGGGACATG 
GTCCGAAAGC TGGTGCCCAG CCGTGACCAG ACCCTGCAGG AAGAGCTTGC CCGGCAGCAG 
CTGAATGAGA GGCTCCGGCG ACAGTTCGCA GCTCAAGCCA ATGCTGTTGG GCCCTGGATC 
CAGGGGAAGG TGGAGGAGGT GGGGCGCCTA GCCACAGGGA TGGCTGGCTC TCTGGAGGAA 
CAGATGGCTG GACTGCGGCA ACAGGAGCAG AACATCATCA ACTACAAGAG CAACATTGAC 
CGGCTGGAGG GTGACCACCA GCTGCTGCAG GAGAGCCTAG TGTTTGACAA CAAGCACACC 
GTCTACAGCA TGGAGCACAT CCGCGTAGGC TGGGAGCAGC TGCTCACCTC CATCGCCCGT 
ACCATCAATG AAGTGGAGAA CCAGGTACTG ACCCGAGATG CCAAGGGCCT GAGCCAGGAG 
CAGCTCAACG AGTTCCGGGC ATCCTTCAAC CACTTTGACC GGGTCAGCGA GGGCCCAATT 
CTGTGGGCGG TGGATGTGGG GGCTGGTGGG GCTGGAGGAC CAAGTCTGAT ATGCACTCAT 
GCACTACAGA AGCGGAACGG GATGATGGAG CCTGATGACT TCCGGGCTTG CCTCATCTCC 
ATGGGCTATG ATCTGGGAGA AGTAGAGTTT GCACGAATTA TGACCATGGT GGACCCCAAC 
GCAGCCGGCG TCGTGACCTT CCAGGCCTTC ATCGACTTCA TGACCCGAGA GACAGCTGAG 
ACTGACACAG CTGAGCAAGT TGTGGCCTCC TTCAAGATCC TGGCAGGAGA TAAGAACTAC 
ATCACTCCTG AAGAGCTGCG GCGGGAGCTC CCTGCTGAGC AGGCCGAGTA CTGCATCCGT 
CGTATGGCAC CCTACAAGGG CTCTGGGGCT CCACCTGGAG CCCTGGACTA CGTGGCCTTC 
TCCAGTGCCC TCTATGGGGA GAGTGACCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTN3 ( XM_847243.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_852336.2 Canis lupus familiaris actinin alpha 3 (ACTN3), transcript variant X2, mRNA.

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CloneID	OCe294887
Clone ID Related Accession (Same CDS sequence)	XM_847243.5
Accession Version	XM_847243.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	alpha-actinin-3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_847243.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGATAG GGCTCTGTTT CCCACCAGCG CGACGCAGGC AGAGGGTCAG GAGGAGGCCT 
GAGAGGCAGC TTGTAGCGGT CAACAGTCAC TATTATTATC GTCATCTCCG TTGTACTCAT 
GAGGAAACAG AGAGGGGCCG TGGCTTTCCC ATGGTCACAC AGCACCCAAG AGGCGGAGCC 
GGAACGGGAA CCCCGAATCT CCGCTATGGG ATCCACCCCG GCGCCCCCAA ATCCTTCAGT 
CCCCTGGTCT CAGGGCAACC TCTTGCCTGG TCCCTACCCA AACGCCCTCG CCCCCATCCG 
GCCCCACCCA CCTCCCCCAT ATTTAGCGCG AATCCGATCC GGGGCTGGGC CGGGCGCTAC 
TTAACGCGGC CCGGGTGTTC TGGAGAGCGC GGAGCGGAGC GAGCCAGGAG CCCCAGCAAG 
ATGATGATGG TTATGCAGCC CGAGGGCCTG GGGGTCGGGG AGGGGCCCTT CGTGGGCGGC 
AGCGGGGGCG GCGAGTACAT GGAACAGGAG GAAGACTGGG ACCGCGACCT ACTGCTCGAC 
CCGGCCTGGG AGAAGCAGCA GCGGAAAACC TTCACTGCCT GGTGCAACTC ACACCTGCGC 
AAGGCTGGCA CCCAGATTGA AAACATAGAG GAGGATTTCC GCAATGGCCT CAAACTCATG 
TTGCTTTTGG AGGTCATTTC AGGAGAGAGG CTACCTAGGC CAGACAAAGG CAAGATGCGC 
TTCCACAAAA TTGCCAATGT CAACAAGGCC CTGGACTTCA TTGCCAGCAA GGGGGTTAAG 
CTGGTGTCCA TTGGTGCTGA AGAGATTGTT GACGGGAACC TGAAGATGAC CCTGGGCATG 
ATCTGGACCA TCATCCTTCG CTTTGCTATC CAGGACATCT CTGTGGAGGA AACCTCTGCC 
AAGGAAGGCT TGCTCCTGTG GTGCCAGCGG AAGACAGCTC CGTACCGCAA CGTCAACGTG 
CAAAACTTCC ACACCAGCTG GAAGGATGGT TTGGCCCTCT GTGCTCTCAT CCACCGACAC 
CGCCCCGATC TCATCGATTA TGCCAAACTA CGCAAGGATG ACCCCATTGG CAATCTAAAC 
ACGGCCTTCG AGGTTGCAGA GAAATACTTG GACATCCCTA GGATGTTGGA TGCAGAAGAC 
ATCGTGAACA CCCCAAAGCC TGATGAAAAG GCCATCATGA CCTATGTTTC CTGCTTCTAC 
CATGCCTTTG CCGGGGCTGA GCAGGCAGAG ACAGCTGCCA ACAGGATCTG CAAGGTGCTG 
GCTGTGAACC AGGAGAACGA GAAGCTGATG GAGGAGTACG AGAAGCTTGC CAGTGAGCTG 
CTGGAATGGA TCCGTCGCAC CATACCATGG CTGGAGAACC GTGTGGGCGA GCCCAGCATG 
AGTGCCATGC AGCGCAAGCT GGAGGACTTT CGGGACTACC GGCGCCTGCA TAAGCCACCC 
CGAGTGCAGG AGAAGTGCCA GCTGGAGATC AATTTCAATA CACTGCAGAC CAAGCTGCGG 
CTGAGCCACC GGCCCGCCTT CATGCCCTCC GAGGGCAAGC TCGTCTCGGA TATCGCCAAT 
GCATGGCGTG GGCTGGAGCA AGTAGAGAAG GGCTATGAGG ACTGGCTGCT CTCAGAGATC 
CGGCGCCTGC AGCGGCTCCA GCACCTGGCT GAGAAATTCC AGCAGAAAGC CTCCTTGCAT 
GAAGCCTGGA CCCGGGGGAA GGAGGATATG CTGAGCCAGC GCGACTACGA GTCGGCCTCC 
CTGCAGGAGG TGCGGGCGTT GCTGCGGCGC CACGAGGCCT TTGAGAGCGA CCTGGCAGCG 
CATCAGGACC GCGTGGAGCA CATCGCCGCG CTGGCCCAGG AGCTCAATGA GCTGGACTAC 
CATGAGGCAG CCTCGGTGAA TAGCCGCTGC CAGGCCATCT GCGACCAGTG GGACAATCTG 
GGCACGCTGA CCCAGAAGAG GAGGGATGCG CTAGAACGGA TGGAGAAGCT CCTGGAGACC 
ATTGACCAGC TGCAACTGGA GTTTGCTCGT CGGGCAGCGC CCTTCAACAA CTGGCTGGAT 
GGTGCCGTGG AGGACCTGCA GGACGTGTGG CTGGTGCACT CAGTGGAGGA AACCCAGAGC 
CTGCTGACTG CACATGAGCA ATTCAAGGCT ACGTTGCCAG AGGCTGACCG AGAGCGAGGA 
GCAATCCTCG GTATCCAGGG TGAGATCCAG AAGATCTGCC AAACGTATGG ACTGCGGCCC 
AGCTCCACCA ACCCCTACAT CAACCTCACA CCACAGGACA TCAACACCAA GTGGGACATG 
GTCCGAAAGC TGGTGCCCAG CCGTGACCAG ACCCTGCAGG AAGAGCTTGC CCGGCAGCAG 
CTGAATGAGA GGCTCCGGCG ACAGTTCGCA GCTCAAGCCA ATGCTGTTGG GCCCTGGATC 
CAGGGGAAGG TGGAGGAGGT GGGGCGCCTA GCCACAGGGA TGGCTGGCTC TCTGGAGGAA 
CAGATGGCTG GACTGCGGCA ACAGGAGCAG AACATCATCA ACTACAAGAG CAACATTGAC 
CGGCTGGAGG GTGACCACCA GCTGCTGCAG GAGAGCCTAG TGTTTGACAA CAAGCACACC 
GTCTACAGCA TGGAGCACAT CCGCGTAGGC TGGGAGCAGC TGCTCACCTC CATCGCCCGT 
ACCATCAATG AAGTGGAGAA CCAGGTACTG ACCCGAGATG CCAAGGGCCT GAGCCAGGAG 
CAGCTCAACG AGTTCCGGGC ATCCTTCAAC CACTTTGACC GGAAGCGGAA CGGGATGATG 
GAGCCTGATG ACTTCCGGGC TTGCCTCATC TCCATGGGCT ATGATCTGGG AGAAGTAGAG 
TTTGCACGAA TTATGACCAT GGTGGACCCC AACGCAGCCG GCGTCGTGAC CTTCCAGGCC 
TTCATCGACT TCATGACCCG AGAGACAGCT GAGACTGACA CAGCTGAGCA AGTTGTGGCC 
TCCTTCAAGA TCCTGGCAGG AGATAAGAAC TACATCACTC CTGAAGAGCT GCGGCGGGAG 
CTCCCTGCTG AGCAGGCCGA GTACTGCATC CGTCGTATGG CACCCTACAA GGGCTCTGGG 
GCTCCACCTG GAGCCCTGGA CTACGTGGCC TTCTCCAGTG CCCTCTATGG GGAGAGTGAC 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_852336.2
CDS	192..3317
Translation	MGIGLCFPPARRRQRVRRRPERQLVAVNSHYYYRHLRCTHEETERGRGFPMVTQHPRGGAGTGTPNLRYGIHPGAPKSFSPLVSGQPLAWSLPKRPRPHPAPPTSPIFSANPIRGWAGRYLTRPGCSGERGAERARSPSKMMMVMQPEGLGVGEGPFVGGSGGGEYMEQEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRNGLKLMLLLEVISGERLPRPDKGKMRFHKIANVNKALDFIASKGVKLVSIGAEEIVDGNLKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYRNVNVQNFHTSWKDGLALCALIHRHRPDLIDYAKLRKDDPIGNLNTAFEVAEKYLDIPRMLDAEDIVNTPKPDEKAIMTYVSCFYHAFAGAEQAETAANRICKVLAVNQENEKLMEEYEKLASELLEWIRRTIPWLENRVGEPSMSAMQRKLEDFRDYRRLHKPPRVQEKCQLEINFNTLQTKLRLSHRPAFMPSEGKLVSDIANAWRGLEQVEKGYEDWLLSEIRRLQRLQHLAEKFQQKASLHEAWTRGKEDMLSQRDYESASLQEVRALLRRHEAFESDLAAHQDRVEHIAALAQELNELDYHEAASVNSRCQAICDQWDNLGTLTQKRRDALERMEKLLETIDQLQLEFARRAAPFNNWLDGAVEDLQDVWLVHSVEETQSLLTAHEQFKATLPEADRERGAILGIQGEIQKICQTYGLRPSSTNPYINLTPQDINTKWDMVRKLVPSRDQTLQEELARQQLNERLRRQFAAQANAVGPWIQGKVEEVGRLATGMAGSLEEQMAGLRQQEQNIINYKSNIDRLEGDHQLLQESLVFDNKHTVYSMEHIRVGWEQLLTSIARTINEVENQVLTRDAKGLSQEQLNEFRASFNHFDRKRNGMMEPDDFRACLISMGYDLGEVEFARIMTMVDPNAAGVVTFQAFIDFMTRETAETDTAEQVVASFKILAGDKNYITPEELRRELPAEQAEYCIRRMAPYKGSGAPPGALDYVAFSSALYGESDL

Target ORF information:

RefSeq Version	XM_847243.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris actinin alpha 3 (ACTN3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_847243.5

ORF Insert Sequence:

ATGGGGATAG GGCTCTGTTT CCCACCAGCG CGACGCAGGC AGAGGGTCAG GAGGAGGCCT 
GAGAGGCAGC TTGTAGCGGT CAACAGTCAC TATTATTATC GTCATCTCCG TTGTACTCAT 
GAGGAAACAG AGAGGGGCCG TGGCTTTCCC ATGGTCACAC AGCACCCAAG AGGCGGAGCC 
GGAACGGGAA CCCCGAATCT CCGCTATGGG ATCCACCCCG GCGCCCCCAA ATCCTTCAGT 
CCCCTGGTCT CAGGGCAACC TCTTGCCTGG TCCCTACCCA AACGCCCTCG CCCCCATCCG 
GCCCCACCCA CCTCCCCCAT ATTTAGCGCG AATCCGATCC GGGGCTGGGC CGGGCGCTAC 
TTAACGCGGC CCGGGTGTTC TGGAGAGCGC GGAGCGGAGC GAGCCAGGAG CCCCAGCAAG 
ATGATGATGG TTATGCAGCC CGAGGGCCTG GGGGTCGGGG AGGGGCCCTT CGTGGGCGGC 
AGCGGGGGCG GCGAGTACAT GGAACAGGAG GAAGACTGGG ACCGCGACCT ACTGCTCGAC 
CCGGCCTGGG AGAAGCAGCA GCGGAAAACC TTCACTGCCT GGTGCAACTC ACACCTGCGC 
AAGGCTGGCA CCCAGATTGA AAACATAGAG GAGGATTTCC GCAATGGCCT CAAACTCATG 
TTGCTTTTGG AGGTCATTTC AGGAGAGAGG CTACCTAGGC CAGACAAAGG CAAGATGCGC 
TTCCACAAAA TTGCCAATGT CAACAAGGCC CTGGACTTCA TTGCCAGCAA GGGGGTTAAG 
CTGGTGTCCA TTGGTGCTGA AGAGATTGTT GACGGGAACC TGAAGATGAC CCTGGGCATG 
ATCTGGACCA TCATCCTTCG CTTTGCTATC CAGGACATCT CTGTGGAGGA AACCTCTGCC 
AAGGAAGGCT TGCTCCTGTG GTGCCAGCGG AAGACAGCTC CGTACCGCAA CGTCAACGTG 
CAAAACTTCC ACACCAGCTG GAAGGATGGT TTGGCCCTCT GTGCTCTCAT CCACCGACAC 
CGCCCCGATC TCATCGATTA TGCCAAACTA CGCAAGGATG ACCCCATTGG CAATCTAAAC 
ACGGCCTTCG AGGTTGCAGA GAAATACTTG GACATCCCTA GGATGTTGGA TGCAGAAGAC 
ATCGTGAACA CCCCAAAGCC TGATGAAAAG GCCATCATGA CCTATGTTTC CTGCTTCTAC 
CATGCCTTTG CCGGGGCTGA GCAGGCAGAG ACAGCTGCCA ACAGGATCTG CAAGGTGCTG 
GCTGTGAACC AGGAGAACGA GAAGCTGATG GAGGAGTACG AGAAGCTTGC CAGTGAGCTG 
CTGGAATGGA TCCGTCGCAC CATACCATGG CTGGAGAACC GTGTGGGCGA GCCCAGCATG 
AGTGCCATGC AGCGCAAGCT GGAGGACTTT CGGGACTACC GGCGCCTGCA TAAGCCACCC 
CGAGTGCAGG AGAAGTGCCA GCTGGAGATC AATTTCAATA CACTGCAGAC CAAGCTGCGG 
CTGAGCCACC GGCCCGCCTT CATGCCCTCC GAGGGCAAGC TCGTCTCGGA TATCGCCAAT 
GCATGGCGTG GGCTGGAGCA AGTAGAGAAG GGCTATGAGG ACTGGCTGCT CTCAGAGATC 
CGGCGCCTGC AGCGGCTCCA GCACCTGGCT GAGAAATTCC AGCAGAAAGC CTCCTTGCAT 
GAAGCCTGGA CCCGGGGGAA GGAGGATATG CTGAGCCAGC GCGACTACGA GTCGGCCTCC 
CTGCAGGAGG TGCGGGCGTT GCTGCGGCGC CACGAGGCCT TTGAGAGCGA CCTGGCAGCG 
CATCAGGACC GCGTGGAGCA CATCGCCGCG CTGGCCCAGG AGCTCAATGA GCTGGACTAC 
CATGAGGCAG CCTCGGTGAA TAGCCGCTGC CAGGCCATCT GCGACCAGTG GGACAATCTG 
GGCACGCTGA CCCAGAAGAG GAGGGATGCG CTAGAACGGA TGGAGAAGCT CCTGGAGACC 
ATTGACCAGC TGCAACTGGA GTTTGCTCGT CGGGCAGCGC CCTTCAACAA CTGGCTGGAT 
GGTGCCGTGG AGGACCTGCA GGACGTGTGG CTGGTGCACT CAGTGGAGGA AACCCAGAGC 
CTGCTGACTG CACATGAGCA ATTCAAGGCT ACGTTGCCAG AGGCTGACCG AGAGCGAGGA 
GCAATCCTCG GTATCCAGGG TGAGATCCAG AAGATCTGCC AAACGTATGG ACTGCGGCCC 
AGCTCCACCA ACCCCTACAT CAACCTCACA CCACAGGACA TCAACACCAA GTGGGACATG 
GTCCGAAAGC TGGTGCCCAG CCGTGACCAG ACCCTGCAGG AAGAGCTTGC CCGGCAGCAG 
CTGAATGAGA GGCTCCGGCG ACAGTTCGCA GCTCAAGCCA ATGCTGTTGG GCCCTGGATC 
CAGGGGAAGG TGGAGGAGGT GGGGCGCCTA GCCACAGGGA TGGCTGGCTC TCTGGAGGAA 
CAGATGGCTG GACTGCGGCA ACAGGAGCAG AACATCATCA ACTACAAGAG CAACATTGAC 
CGGCTGGAGG GTGACCACCA GCTGCTGCAG GAGAGCCTAG TGTTTGACAA CAAGCACACC 
GTCTACAGCA TGGAGCACAT CCGCGTAGGC TGGGAGCAGC TGCTCACCTC CATCGCCCGT 
ACCATCAATG AAGTGGAGAA CCAGGTACTG ACCCGAGATG CCAAGGGCCT GAGCCAGGAG 
CAGCTCAACG AGTTCCGGGC ATCCTTCAAC CACTTTGACC GGAAGCGGAA CGGGATGATG 
GAGCCTGATG ACTTCCGGGC TTGCCTCATC TCCATGGGCT ATGATCTGGG AGAAGTAGAG 
TTTGCACGAA TTATGACCAT GGTGGACCCC AACGCAGCCG GCGTCGTGAC CTTCCAGGCC 
TTCATCGACT TCATGACCCG AGAGACAGCT GAGACTGACA CAGCTGAGCA AGTTGTGGCC 
TCCTTCAAGA TCCTGGCAGG AGATAAGAAC TACATCACTC CTGAAGAGCT GCGGCGGGAG 
CTCCCTGCTG AGCAGGCCGA GTACTGCATC CGTCGTATGG CACCCTACAA GGGCTCTGGG 
GCTCCACCTG GAGCCCTGGA CTACGTGGCC TTCTCCAGTG CCCTCTATGG GGAGAGTGAC 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ACTN3 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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