PC cDNA ORF clone, Canis lupus familiaris(Dog)

The following PC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe20283 XM_005631415.3
Latest version!
Canis lupus familiaris pyruvate carboxylase (PC), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe20284 XM_540825.6
Latest version!
Canis lupus familiaris pyruvate carboxylase (PC), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe20284 XM_005631416.3
Latest version!
Canis lupus familiaris pyruvate carboxylase (PC), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
OCe20283 XM_005631415.2 Canis lupus familiaris pyruvate carboxylase (PC), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe20284 XM_005631416.2 Canis lupus familiaris pyruvate carboxylase (PC), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe20284 XM_540825.5 Canis lupus familiaris pyruvate carboxylase (PC), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe20283
    Clone ID Related Accession (Same CDS sequence) XM_005631415.2 , XM_005631415.3
    Accession Version XM_005631415.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3573bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product pyruvate carboxylase, mitochondrial isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005631415.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCTCTC TGGGACTCTG CAGACCAACT GCCACGATGC TGAAGTTCCA AACCGTCCGT 
    GGGGGCCTGA GGCTCCTGGG TATCCACCGA AGCTCCACTG CCCCTGCTGC CTCCCCAAAT
    GTCCGGCGCC TGGAGTACAA GCCCATCAAG AAAGTTATGG TGGCCAACAG AGGTGAGATT
    GCTATCCGTG TGTTCCGGGC CTGCACAGAG CTGGGCATCC GCACTGTAGC TGTCTACTCG
    GAGCAGGACA CAGGCCAGAT GCACCGGCAG AAAGCAGATG AAGCCTACCT CATCGGCCGC
    GGCCTTGCCC CAGTGCAGGC CTATCTGCAC ATCCCAGACA TCATCAAGGT GGCCAAGGAA
    AACAATGTGG ATGCAGTGCA CCCTGGCTAC GGGTTCCTGT CAGAGCGAGC AGACTTTGCC
    CAAGCCTGCC AGGATGCAGG GGTTCGGTTT ATTGGGCCGA GCCCTGAGGT GGTCCGCAAG
    ATGGGCGATA AGGTGGAGGC CCGGGCCATC GCCATTGCTG CAGGTGTCCC TGTGGTCCCT
    GGCACAGATG CCCCCATCAC TTCCTTGCAC GAGGCCCATG AATTCTCTAA CACCTATGGC
    TTCCCCATTA TCTTCAAGGC CGCCTATGGA GGTGGGGGAC GTGGCATGAG GGTCGTGCAC
    AGTTATGAGG AGCTGGAGGA AAGCTATAAC CGGGCCTACT CGGAGGCCCT GGCCGCCTTT
    GGGAATGGGG CGCTGTTTGT GGAGAAGTTC ATTGAGAAGC CACGCCACAT CGAGGTGCAG
    ATCCTGGGAG ACCAGTACGG GAATATTCTG CACCTCTATG AGCGAGACTG TTCCATCCAA
    CGGCGGCACC AGAAAGTGGT TGAGATTGCT CCTGCAGCCC ACCTGGATCC CCAGCTTCGG
    ACACGGCTCA CCAGTGACTC TGTCAAACTT GCTAAGCAGG TGGGCTACGA GAACGCAGGC
    ACGGTGGAGT TCCTGGTGGA CCGGCATGGC AGGCACTACT TCATCGAGGT CAACTCACGC
    CTGCAGGTGG AGCACACAGT CACCGAGGAG ATCACAGATG TGGATCTGGT TCACGCCCAG
    ATCCATGTGG CCGAGGGCCG GAGCCTGCCT GACCTGGGCC TGCGGCAGGA GAATATCCGT
    ATCAACGGTT GTGCCATTCA GTGCCGGGTC ACCACCGAGG ACCCTGCACG CAGCTTCCAG
    CCAGACACTG GCCGAATCGA GGTGTTCCGG AGCGGAGAGG GCATGGGCAT CCGCCTGGAT
    AATGCATCTG CCTTCCAAGG AGCTGTCATC TCCCCGCACT ATGACTCCCT GCTCGTCAAA
    GTCATTGCTC ATGGCAAAGA CCACCCCACG GCCGCCACCA AGATGAGCAG AGCCCTTGCG
    GAGTTCCGAG TCCGAGGTGT AAAGACCAAC ATCCCCTTCC TGCAGAATGT GCTCAACAAC
    CAGCAGTTCC TGGCAGGCAC CGTGGACACC CAGTTCATCG ATGAGAACCC GGAGCTTTTC
    CAGCTGCGGC CTGCGCAGAA CCGGGCTCAG AAGCTGCTGC ACTACCTCGG TCATGTCATG
    GTGAACGGAC CAACCACCCC AATCCCTGTC AAGGCCAGCC CCAGCCCCAC GGACCCTGTT
    GTCCCTGCGG TGCCCATAGG CCCACCCCCA GCTGGTTTCA GAGACATCCT GCTGCGGGAG
    GGGCCTGAGG GCTTTGCTCG AGCTGTGAGG AACCACCAGG GGCTGCTGCT CATGGACACA
    ACCTTCAGGG ATGCCCACCA GTCGCTGCTG GCCACTCGTG TGCGAACCCA TGATCTCAAA
    AAGATTGCCC CCTACGTTGC CCACAACTTC AGCAAGCTCT TCAGCATAGA GAACTGGGGA
    GGAGCCACGT TTGATGTTGC CATGCGCTTT CTGTACGAGT GCCCCTGGCG GCGGCTGCAG
    GAGCTCCGGG AGCTCATCCC CAACATCCCG TTTCAGATGC TGCTGCGGGG GGCCAATGCT
    GTGGGCTACA CCAACTACCC TGACAATGTG GTGTTCAAGT TCTGTGAGGT AGCCAAGGAG
    AATGGCATGG ACGTCTTCCG GGTTTTTGAC TCCCTCAACT ACATGCCAAA TCTGCTACTG
    GGCATGGAGG CTGCAGGCAA TGCTGGTGGC GTGGTGGAGG CTGCCATCTC CTACACTGGT
    GACGTGGCCG ACCCCAACCG CACCAAATAC TCACTGCAGT ACTACATGGG CTTGGCGGAA
    GAGCTGGTGC GAGCTGGCAC CCATATCCTG TGTATCAAGG ACATGGCAGG GCTGCTAAAG
    CCTACAGCCT GCACCATGCT GGTCAGCTCC CTCAGGGACC GGTTCCCCGA CCTTCCACTG
    CACATCCACA CCCATGACAC ATCAGGGGCA GGTGTGGCAG CTATGTTGGC CTGTGCCCAG
    GCTGGGGCTG ATGTGGTAGA TGTGGCATCT GACTCCATGT CCGGGATGAC TTCACAGCCC
    AGCATGGGGG CCCTGGTGGC CTGCACCCGA GGGACTCCCC TGGACACAGG GGTGCCCCTG
    GAACGTGTGT TTGACTACAG TGAGTACTGG GAGGGGGCCC GGGGATTGTA TGCGGCCTTT
    GACTGCACGG CCACCATGAA GTCTGGCAAT TCGGACGTGT ATGAGAACGA GATCCCAGGC
    GGCCAGTACA CCAATCTGCA CTTCCAGGCA CACAGCATGG GGCTTGGCTC CAAGTTCAAG
    GAAGTCAAGA AGGCCTATGT GGAGGCCAAC CAGATGCTGG GCGATCTCAT CAAGGTGACC
    CCGTCCTCCA AGATCGTGGG CGACCTGGCC CAGTTCATGG TACAGAATGG GCTGAGCCGG
    GCAGAGGCTG AAGCCCAGGC AGAAGAATTG TCCTTCCCCC GTTCCGTGGT GGAGTTCCTG
    CAGGGCTACA TTGGCATTCC CCACGGGGGC TTCCCTGAGC CCCTTCGCTC TAAGGTGCTA
    AAGGACCTGC CAAGGGTAGA AGGGCGGCCA GGAGCCTCCC TCCCCCCACT GGATCTGCAG
    CTGCTGGAGA AGGAGCTGAC TGAACGGCAT GGGGAGGAGG TGACCCCAGA AGATGTGCTC
    TCAGCAGCCA TATATCCAGA CGTCTTTGCC CACTTCAAGG ACTTCACAGC TACCTTTGGC
    CCCCTGGATA GCCTCAATAC CCGCCTCTTC CTGCAGGGAC CCAAAATTGC AGAGGAGTTT
    GAGGTGGAGC TGGAGCGGGG CAAGACACTG CACATCAAGG CTCTGGCCAT AAGTGACCTG
    AACCGGGCTG GCCAGAGGCA GGTCTTCTTT GAGCTCAATG GACAGCTGCG GTCCATTCTG
    GTCAAGGACA CTCAGGCCAT GAAGGAAATG CACTTCCACC CCAAGGCCCT GAAGGATGTG
    AAGGGCCAGA TTGGGGCACC CATGCCTGGG AAGGTGATAG ACATCAAGGT GGCAGCTGGG
    GCCAAGGTGG CCAAGGGCCA GCCCCTCTGT GTGCTCAGTG CCATGAAGAT GGAGACTGTG
    GTGACTTCAC CCATGGAGGG CACTGTCCGC AAGGTCCACG TAACCACAGA CATGACACTG
    GAGGGCGATG ACCTCATCCT GGAGATTGAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005631472.1
    CDS108..3680
    Translation

    Target ORF information:

    RefSeq Version XM_005631415.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris pyruvate carboxylase (PC), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005631415.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCTCTC TGGGACTCTG CAGACCAACT GCCACGATGC TGAAGTTCCA AACCGTCCGT 
    GGGGGCCTGA GGCTCCTGGG TATCCACCGA AGCTCCACTG CCCCTGCTGC CTCCCCAAAT
    GTCCGGCGCC TGGAGTACAA GCCCATCAAG AAAGTTATGG TGGCCAACAG AGGTGAGATT
    GCTATCCGTG TGTTCCGGGC CTGCACAGAG CTGGGCATCC GCACTGTAGC TGTCTACTCG
    GAGCAGGACA CAGGCCAGAT GCACCGGCAG AAAGCAGATG AAGCCTACCT CATCGGCCGC
    GGCCTTGCCC CAGTGCAGGC CTATCTGCAC ATCCCAGACA TCATCAAGGT GGCCAAGGAA
    AACAATGTGG ATGCAGTGCA CCCTGGCTAC GGGTTCCTGT CAGAGCGAGC AGACTTTGCC
    CAAGCCTGCC AGGATGCAGG GGTTCGGTTT ATTGGGCCGA GCCCTGAGGT GGTCCGCAAG
    ATGGGCGATA AGGTGGAGGC CCGGGCCATC GCCATTGCTG CAGGTGTCCC TGTGGTCCCT
    GGCACAGATG CCCCCATCAC TTCCTTGCAC GAGGCCCATG AATTCTCTAA CACCTATGGC
    TTCCCCATTA TCTTCAAGGC CGCCTATGGA GGTGGGGGAC GTGGCATGAG GGTCGTGCAC
    AGTTATGAGG AGCTGGAGGA AAGCTATAAC CGGGCCTACT CGGAGGCCCT GGCCGCCTTT
    GGGAATGGGG CGCTGTTTGT GGAGAAGTTC ATTGAGAAGC CACGCCACAT CGAGGTGCAG
    ATCCTGGGAG ACCAGTACGG GAATATTCTG CACCTCTATG AGCGAGACTG TTCCATCCAA
    CGGCGGCACC AGAAAGTGGT TGAGATTGCT CCTGCAGCCC ACCTGGATCC CCAGCTTCGG
    ACACGGCTCA CCAGTGACTC TGTCAAACTT GCTAAGCAGG TGGGCTACGA GAACGCAGGC
    ACGGTGGAGT TCCTGGTGGA CCGGCATGGC AGGCACTACT TCATCGAGGT CAACTCACGC
    CTGCAGGTGG AGCACACAGT CACCGAGGAG ATCACAGATG TGGATCTGGT TCACGCCCAG
    ATCCATGTGG CCGAGGGCCG GAGCCTGCCT GACCTGGGCC TGCGGCAGGA GAATATCCGT
    ATCAACGGTT GTGCCATTCA GTGCCGGGTC ACCACCGAGG ACCCTGCACG CAGCTTCCAG
    CCAGACACTG GCCGAATCGA GGTGTTCCGG AGCGGAGAGG GCATGGGCAT CCGCCTGGAT
    AATGCATCTG CCTTCCAAGG AGCTGTCATC TCCCCGCACT ATGACTCCCT GCTCGTCAAA
    GTCATTGCTC ATGGCAAAGA CCACCCCACG GCCGCCACCA AGATGAGCAG AGCCCTTGCG
    GAGTTCCGAG TCCGAGGTGT AAAGACCAAC ATCCCCTTCC TGCAGAATGT GCTCAACAAC
    CAGCAGTTCC TGGCAGGCAC CGTGGACACC CAGTTCATCG ATGAGAACCC GGAGCTTTTC
    CAGCTGCGGC CTGCGCAGAA CCGGGCTCAG AAGCTGCTGC ACTACCTCGG TCATGTCATG
    GTGAACGGAC CAACCACCCC AATCCCTGTC AAGGCCAGCC CCAGCCCCAC GGACCCTGTT
    GTCCCTGCGG TGCCCATAGG CCCACCCCCA GCTGGTTTCA GAGACATCCT GCTGCGGGAG
    GGGCCTGAGG GCTTTGCTCG AGCTGTGAGG AACCACCAGG GGCTGCTGCT CATGGACACA
    ACCTTCAGGG ATGCCCACCA GTCGCTGCTG GCCACTCGTG TGCGAACCCA TGATCTCAAA
    AAGATTGCCC CCTACGTTGC CCACAACTTC AGCAAGCTCT TCAGCATAGA GAACTGGGGA
    GGAGCCACGT TTGATGTTGC CATGCGCTTT CTGTACGAGT GCCCCTGGCG GCGGCTGCAG
    GAGCTCCGGG AGCTCATCCC CAACATCCCG TTTCAGATGC TGCTGCGGGG GGCCAATGCT
    GTGGGCTACA CCAACTACCC TGACAATGTG GTGTTCAAGT TCTGTGAGGT AGCCAAGGAG
    AATGGCATGG ACGTCTTCCG GGTTTTTGAC TCCCTCAACT ACATGCCAAA TCTGCTACTG
    GGCATGGAGG CTGCAGGCAA TGCTGGTGGC GTGGTGGAGG CTGCCATCTC CTACACTGGT
    GACGTGGCCG ACCCCAACCG CACCAAATAC TCACTGCAGT ACTACATGGG CTTGGCGGAA
    GAGCTGGTGC GAGCTGGCAC CCATATCCTG TGTATCAAGG ACATGGCAGG GCTGCTAAAG
    CCTACAGCCT GCACCATGCT GGTCAGCTCC CTCAGGGACC GGTTCCCCGA CCTTCCACTG
    CACATCCACA CCCATGACAC ATCAGGGGCA GGTGTGGCAG CTATGTTGGC CTGTGCCCAG
    GCTGGGGCTG ATGTGGTAGA TGTGGCATCT GACTCCATGT CCGGGATGAC TTCACAGCCC
    AGCATGGGGG CCCTGGTGGC CTGCACCCGA GGGACTCCCC TGGACACAGG GGTGCCCCTG
    GAACGTGTGT TTGACTACAG TGAGTACTGG GAGGGGGCCC GGGGATTGTA TGCGGCCTTT
    GACTGCACGG CCACCATGAA GTCTGGCAAT TCGGACGTGT ATGAGAACGA GATCCCAGGC
    GGCCAGTACA CCAATCTGCA CTTCCAGGCA CACAGCATGG GGCTTGGCTC CAAGTTCAAG
    GAAGTCAAGA AGGCCTATGT GGAGGCCAAC CAGATGCTGG GCGATCTCAT CAAGGTGACC
    CCGTCCTCCA AGATCGTGGG CGACCTGGCC CAGTTCATGG TACAGAATGG GCTGAGCCGG
    GCAGAGGCTG AAGCCCAGGC AGAAGAATTG TCCTTCCCCC GTTCCGTGGT GGAGTTCCTG
    CAGGGCTACA TTGGCATTCC CCACGGGGGC TTCCCTGAGC CCCTTCGCTC TAAGGTGCTA
    AAGGACCTGC CAAGGGTAGA AGGGCGGCCA GGAGCCTCCC TCCCCCCACT GGATCTGCAG
    CTGCTGGAGA AGGAGCTGAC TGAACGGCAT GGGGAGGAGG TGACCCCAGA AGATGTGCTC
    TCAGCAGCCA TATATCCAGA CGTCTTTGCC CACTTCAAGG ACTTCACAGC TACCTTTGGC
    CCCCTGGATA GCCTCAATAC CCGCCTCTTC CTGCAGGGAC CCAAAATTGC AGAGGAGTTT
    GAGGTGGAGC TGGAGCGGGG CAAGACACTG CACATCAAGG CTCTGGCCAT AAGTGACCTG
    AACCGGGCTG GCCAGAGGCA GGTCTTCTTT GAGCTCAATG GACAGCTGCG GTCCATTCTG
    GTCAAGGACA CTCAGGCCAT GAAGGAAATG CACTTCCACC CCAAGGCCCT GAAGGATGTG
    AAGGGCCAGA TTGGGGCACC CATGCCTGGG AAGGTGATAG ACATCAAGGT GGCAGCTGGG
    GCCAAGGTGG CCAAGGGCCA GCCCCTCTGT GTGCTCAGTG CCATGAAGAT GGAGACTGTG
    GTGACTTCAC CCATGGAGGG CACTGTCCGC AAGGTCCACG TAACCACAGA CATGACACTG
    GAGGGCGATG ACCTCATCCT GGAGATTGAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20283
    Clone ID Related Accession (Same CDS sequence) XM_005631415.2 , XM_005631415.3
    Accession Version XM_005631415.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3573bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pyruvate carboxylase, mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005631415.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCTCTC TGGGACTCTG CAGACCAACT GCCACGATGC TGAAGTTCCA AACCGTCCGT 
    GGGGGCCTGA GGCTCCTGGG TATCCACCGA AGCTCCACTG CCCCTGCTGC CTCCCCAAAT
    GTCCGGCGCC TGGAGTACAA GCCCATCAAG AAAGTTATGG TGGCCAACAG AGGTGAGATT
    GCTATCCGTG TGTTCCGGGC CTGCACAGAG CTGGGCATCC GCACTGTAGC TGTCTACTCG
    GAGCAGGACA CAGGCCAGAT GCACCGGCAG AAAGCAGATG AAGCCTACCT CATCGGCCGC
    GGCCTTGCCC CAGTGCAGGC CTATCTGCAC ATCCCAGACA TCATCAAGGT GGCCAAGGAA
    AACAATGTGG ATGCAGTGCA CCCTGGCTAC GGGTTCCTGT CAGAGCGAGC AGACTTTGCC
    CAAGCCTGCC AGGATGCAGG GGTTCGGTTT ATTGGGCCGA GCCCTGAGGT GGTCCGCAAG
    ATGGGCGATA AGGTGGAGGC CCGGGCCATC GCCATTGCTG CAGGTGTCCC TGTGGTCCCT
    GGCACAGATG CCCCCATCAC TTCCTTGCAC GAGGCCCATG AATTCTCTAA CACCTATGGC
    TTCCCCATTA TCTTCAAGGC CGCCTATGGA GGTGGGGGAC GTGGCATGAG GGTCGTGCAC
    AGTTATGAGG AGCTGGAGGA AAGCTATAAC CGGGCCTACT CGGAGGCCCT GGCCGCCTTT
    GGGAATGGGG CGCTGTTTGT GGAGAAGTTC ATTGAGAAGC CACGCCACAT CGAGGTGCAG
    ATCCTGGGAG ACCAGTACGG GAATATTCTG CACCTCTATG AGCGAGACTG TTCCATCCAA
    CGGCGGCACC AGAAAGTGGT TGAGATTGCT CCTGCAGCCC ACCTGGATCC CCAGCTTCGG
    ACACGGCTCA CCAGTGACTC TGTCAAACTT GCTAAGCAGG TGGGCTACGA GAACGCAGGC
    ACGGTGGAGT TCCTGGTGGA CCGGCATGGC AGGCACTACT TCATCGAGGT CAACTCACGC
    CTGCAGGTGG AGCACACAGT CACCGAGGAG ATCACAGATG TGGATCTGGT TCACGCCCAG
    ATCCATGTGG CCGAGGGCCG GAGCCTGCCT GACCTGGGCC TGCGGCAGGA GAATATCCGT
    ATCAACGGTT GTGCCATTCA GTGCCGGGTC ACCACCGAGG ACCCTGCACG CAGCTTCCAG
    CCAGACACTG GCCGAATCGA GGTGTTCCGG AGCGGAGAGG GCATGGGCAT CCGCCTGGAT
    AATGCATCTG CCTTCCAAGG AGCTGTCATC TCCCCGCACT ATGACTCCCT GCTCGTCAAA
    GTCATTGCTC ATGGCAAAGA CCACCCCACG GCCGCCACCA AGATGAGCAG AGCCCTTGCG
    GAGTTCCGAG TCCGAGGTGT AAAGACCAAC ATCCCCTTCC TGCAGAATGT GCTCAACAAC
    CAGCAGTTCC TGGCAGGCAC CGTGGACACC CAGTTCATCG ATGAGAACCC GGAGCTTTTC
    CAGCTGCGGC CTGCGCAGAA CCGGGCTCAG AAGCTGCTGC ACTACCTCGG TCATGTCATG
    GTGAACGGAC CAACCACCCC AATCCCTGTC AAGGCCAGCC CCAGCCCCAC GGACCCTGTT
    GTCCCTGCGG TGCCCATAGG CCCACCCCCA GCTGGTTTCA GAGACATCCT GCTGCGGGAG
    GGGCCTGAGG GCTTTGCTCG AGCTGTGAGG AACCACCAGG GGCTGCTGCT CATGGACACA
    ACCTTCAGGG ATGCCCACCA GTCGCTGCTG GCCACTCGTG TGCGAACCCA TGATCTCAAA
    AAGATTGCCC CCTACGTTGC CCACAACTTC AGCAAGCTCT TCAGCATAGA GAACTGGGGA
    GGAGCCACGT TTGATGTTGC CATGCGCTTT CTGTACGAGT GCCCCTGGCG GCGGCTGCAG
    GAGCTCCGGG AGCTCATCCC CAACATCCCG TTTCAGATGC TGCTGCGGGG GGCCAATGCT
    GTGGGCTACA CCAACTACCC TGACAATGTG GTGTTCAAGT TCTGTGAGGT AGCCAAGGAG
    AATGGCATGG ACGTCTTCCG GGTTTTTGAC TCCCTCAACT ACATGCCAAA TCTGCTACTG
    GGCATGGAGG CTGCAGGCAA TGCTGGTGGC GTGGTGGAGG CTGCCATCTC CTACACTGGT
    GACGTGGCCG ACCCCAACCG CACCAAATAC TCACTGCAGT ACTACATGGG CTTGGCGGAA
    GAGCTGGTGC GAGCTGGCAC CCATATCCTG TGTATCAAGG ACATGGCAGG GCTGCTAAAG
    CCTACAGCCT GCACCATGCT GGTCAGCTCC CTCAGGGACC GGTTCCCCGA CCTTCCACTG
    CACATCCACA CCCATGACAC ATCAGGGGCA GGTGTGGCAG CTATGTTGGC CTGTGCCCAG
    GCTGGGGCTG ATGTGGTAGA TGTGGCATCT GACTCCATGT CCGGGATGAC TTCACAGCCC
    AGCATGGGGG CCCTGGTGGC CTGCACCCGA GGGACTCCCC TGGACACAGG GGTGCCCCTG
    GAACGTGTGT TTGACTACAG TGAGTACTGG GAGGGGGCCC GGGGATTGTA TGCGGCCTTT
    GACTGCACGG CCACCATGAA GTCTGGCAAT TCGGACGTGT ATGAGAACGA GATCCCAGGC
    GGCCAGTACA CCAATCTGCA CTTCCAGGCA CACAGCATGG GGCTTGGCTC CAAGTTCAAG
    GAAGTCAAGA AGGCCTATGT GGAGGCCAAC CAGATGCTGG GCGATCTCAT CAAGGTGACC
    CCGTCCTCCA AGATCGTGGG CGACCTGGCC CAGTTCATGG TACAGAATGG GCTGAGCCGG
    GCAGAGGCTG AAGCCCAGGC AGAAGAATTG TCCTTCCCCC GTTCCGTGGT GGAGTTCCTG
    CAGGGCTACA TTGGCATTCC CCACGGGGGC TTCCCTGAGC CCCTTCGCTC TAAGGTGCTA
    AAGGACCTGC CAAGGGTAGA AGGGCGGCCA GGAGCCTCCC TCCCCCCACT GGATCTGCAG
    CTGCTGGAGA AGGAGCTGAC TGAACGGCAT GGGGAGGAGG TGACCCCAGA AGATGTGCTC
    TCAGCAGCCA TATATCCAGA CGTCTTTGCC CACTTCAAGG ACTTCACAGC TACCTTTGGC
    CCCCTGGATA GCCTCAATAC CCGCCTCTTC CTGCAGGGAC CCAAAATTGC AGAGGAGTTT
    GAGGTGGAGC TGGAGCGGGG CAAGACACTG CACATCAAGG CTCTGGCCAT AAGTGACCTG
    AACCGGGCTG GCCAGAGGCA GGTCTTCTTT GAGCTCAATG GACAGCTGCG GTCCATTCTG
    GTCAAGGACA CTCAGGCCAT GAAGGAAATG CACTTCCACC CCAAGGCCCT GAAGGATGTG
    AAGGGCCAGA TTGGGGCACC CATGCCTGGG AAGGTGATAG ACATCAAGGT GGCAGCTGGG
    GCCAAGGTGG CCAAGGGCCA GCCCCTCTGT GTGCTCAGTG CCATGAAGAT GGAGACTGTG
    GTGACTTCAC CCATGGAGGG CACTGTCCGC AAGGTCCACG TAACCACAGA CATGACACTG
    GAGGGCGATG ACCTCATCCT GGAGATTGAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005631472.1
    CDS134..3706
    Translation

    Target ORF information:

    RefSeq Version XM_005631415.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pyruvate carboxylase (PC), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005631415.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCTCTC TGGGACTCTG CAGACCAACT GCCACGATGC TGAAGTTCCA AACCGTCCGT 
    GGGGGCCTGA GGCTCCTGGG TATCCACCGA AGCTCCACTG CCCCTGCTGC CTCCCCAAAT
    GTCCGGCGCC TGGAGTACAA GCCCATCAAG AAAGTTATGG TGGCCAACAG AGGTGAGATT
    GCTATCCGTG TGTTCCGGGC CTGCACAGAG CTGGGCATCC GCACTGTAGC TGTCTACTCG
    GAGCAGGACA CAGGCCAGAT GCACCGGCAG AAAGCAGATG AAGCCTACCT CATCGGCCGC
    GGCCTTGCCC CAGTGCAGGC CTATCTGCAC ATCCCAGACA TCATCAAGGT GGCCAAGGAA
    AACAATGTGG ATGCAGTGCA CCCTGGCTAC GGGTTCCTGT CAGAGCGAGC AGACTTTGCC
    CAAGCCTGCC AGGATGCAGG GGTTCGGTTT ATTGGGCCGA GCCCTGAGGT GGTCCGCAAG
    ATGGGCGATA AGGTGGAGGC CCGGGCCATC GCCATTGCTG CAGGTGTCCC TGTGGTCCCT
    GGCACAGATG CCCCCATCAC TTCCTTGCAC GAGGCCCATG AATTCTCTAA CACCTATGGC
    TTCCCCATTA TCTTCAAGGC CGCCTATGGA GGTGGGGGAC GTGGCATGAG GGTCGTGCAC
    AGTTATGAGG AGCTGGAGGA AAGCTATAAC CGGGCCTACT CGGAGGCCCT GGCCGCCTTT
    GGGAATGGGG CGCTGTTTGT GGAGAAGTTC ATTGAGAAGC CACGCCACAT CGAGGTGCAG
    ATCCTGGGAG ACCAGTACGG GAATATTCTG CACCTCTATG AGCGAGACTG TTCCATCCAA
    CGGCGGCACC AGAAAGTGGT TGAGATTGCT CCTGCAGCCC ACCTGGATCC CCAGCTTCGG
    ACACGGCTCA CCAGTGACTC TGTCAAACTT GCTAAGCAGG TGGGCTACGA GAACGCAGGC
    ACGGTGGAGT TCCTGGTGGA CCGGCATGGC AGGCACTACT TCATCGAGGT CAACTCACGC
    CTGCAGGTGG AGCACACAGT CACCGAGGAG ATCACAGATG TGGATCTGGT TCACGCCCAG
    ATCCATGTGG CCGAGGGCCG GAGCCTGCCT GACCTGGGCC TGCGGCAGGA GAATATCCGT
    ATCAACGGTT GTGCCATTCA GTGCCGGGTC ACCACCGAGG ACCCTGCACG CAGCTTCCAG
    CCAGACACTG GCCGAATCGA GGTGTTCCGG AGCGGAGAGG GCATGGGCAT CCGCCTGGAT
    AATGCATCTG CCTTCCAAGG AGCTGTCATC TCCCCGCACT ATGACTCCCT GCTCGTCAAA
    GTCATTGCTC ATGGCAAAGA CCACCCCACG GCCGCCACCA AGATGAGCAG AGCCCTTGCG
    GAGTTCCGAG TCCGAGGTGT AAAGACCAAC ATCCCCTTCC TGCAGAATGT GCTCAACAAC
    CAGCAGTTCC TGGCAGGCAC CGTGGACACC CAGTTCATCG ATGAGAACCC GGAGCTTTTC
    CAGCTGCGGC CTGCGCAGAA CCGGGCTCAG AAGCTGCTGC ACTACCTCGG TCATGTCATG
    GTGAACGGAC CAACCACCCC AATCCCTGTC AAGGCCAGCC CCAGCCCCAC GGACCCTGTT
    GTCCCTGCGG TGCCCATAGG CCCACCCCCA GCTGGTTTCA GAGACATCCT GCTGCGGGAG
    GGGCCTGAGG GCTTTGCTCG AGCTGTGAGG AACCACCAGG GGCTGCTGCT CATGGACACA
    ACCTTCAGGG ATGCCCACCA GTCGCTGCTG GCCACTCGTG TGCGAACCCA TGATCTCAAA
    AAGATTGCCC CCTACGTTGC CCACAACTTC AGCAAGCTCT TCAGCATAGA GAACTGGGGA
    GGAGCCACGT TTGATGTTGC CATGCGCTTT CTGTACGAGT GCCCCTGGCG GCGGCTGCAG
    GAGCTCCGGG AGCTCATCCC CAACATCCCG TTTCAGATGC TGCTGCGGGG GGCCAATGCT
    GTGGGCTACA CCAACTACCC TGACAATGTG GTGTTCAAGT TCTGTGAGGT AGCCAAGGAG
    AATGGCATGG ACGTCTTCCG GGTTTTTGAC TCCCTCAACT ACATGCCAAA TCTGCTACTG
    GGCATGGAGG CTGCAGGCAA TGCTGGTGGC GTGGTGGAGG CTGCCATCTC CTACACTGGT
    GACGTGGCCG ACCCCAACCG CACCAAATAC TCACTGCAGT ACTACATGGG CTTGGCGGAA
    GAGCTGGTGC GAGCTGGCAC CCATATCCTG TGTATCAAGG ACATGGCAGG GCTGCTAAAG
    CCTACAGCCT GCACCATGCT GGTCAGCTCC CTCAGGGACC GGTTCCCCGA CCTTCCACTG
    CACATCCACA CCCATGACAC ATCAGGGGCA GGTGTGGCAG CTATGTTGGC CTGTGCCCAG
    GCTGGGGCTG ATGTGGTAGA TGTGGCATCT GACTCCATGT CCGGGATGAC TTCACAGCCC
    AGCATGGGGG CCCTGGTGGC CTGCACCCGA GGGACTCCCC TGGACACAGG GGTGCCCCTG
    GAACGTGTGT TTGACTACAG TGAGTACTGG GAGGGGGCCC GGGGATTGTA TGCGGCCTTT
    GACTGCACGG CCACCATGAA GTCTGGCAAT TCGGACGTGT ATGAGAACGA GATCCCAGGC
    GGCCAGTACA CCAATCTGCA CTTCCAGGCA CACAGCATGG GGCTTGGCTC CAAGTTCAAG
    GAAGTCAAGA AGGCCTATGT GGAGGCCAAC CAGATGCTGG GCGATCTCAT CAAGGTGACC
    CCGTCCTCCA AGATCGTGGG CGACCTGGCC CAGTTCATGG TACAGAATGG GCTGAGCCGG
    GCAGAGGCTG AAGCCCAGGC AGAAGAATTG TCCTTCCCCC GTTCCGTGGT GGAGTTCCTG
    CAGGGCTACA TTGGCATTCC CCACGGGGGC TTCCCTGAGC CCCTTCGCTC TAAGGTGCTA
    AAGGACCTGC CAAGGGTAGA AGGGCGGCCA GGAGCCTCCC TCCCCCCACT GGATCTGCAG
    CTGCTGGAGA AGGAGCTGAC TGAACGGCAT GGGGAGGAGG TGACCCCAGA AGATGTGCTC
    TCAGCAGCCA TATATCCAGA CGTCTTTGCC CACTTCAAGG ACTTCACAGC TACCTTTGGC
    CCCCTGGATA GCCTCAATAC CCGCCTCTTC CTGCAGGGAC CCAAAATTGC AGAGGAGTTT
    GAGGTGGAGC TGGAGCGGGG CAAGACACTG CACATCAAGG CTCTGGCCAT AAGTGACCTG
    AACCGGGCTG GCCAGAGGCA GGTCTTCTTT GAGCTCAATG GACAGCTGCG GTCCATTCTG
    GTCAAGGACA CTCAGGCCAT GAAGGAAATG CACTTCCACC CCAAGGCCCT GAAGGATGTG
    AAGGGCCAGA TTGGGGCACC CATGCCTGGG AAGGTGATAG ACATCAAGGT GGCAGCTGGG
    GCCAAGGTGG CCAAGGGCCA GCCCCTCTGT GTGCTCAGTG CCATGAAGAT GGAGACTGTG
    GTGACTTCAC CCATGGAGGG CACTGTCCGC AAGGTCCACG TAACCACAGA CATGACACTG
    GAGGGCGATG ACCTCATCCT GGAGATTGAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20284
    Clone ID Related Accession (Same CDS sequence) XM_540825.6 , XM_005631416.2 , XM_005631416.3 , XM_540825.5
    Accession Version XM_540825.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3537bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pyruvate carboxylase, mitochondrial isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_540825.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCTGAAGT TCCAAACCGT CCGTGGGGGC CTGAGGCTCC TGGGTATCCA CCGAAGCTCC 
    ACTGCCCCTG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAAGTT
    ATGGTGGCCA ACAGAGGTGA GATTGCTATC CGTGTGTTCC GGGCCTGCAC AGAGCTGGGC
    ATCCGCACTG TAGCTGTCTA CTCGGAGCAG GACACAGGCC AGATGCACCG GCAGAAAGCA
    GATGAAGCCT ACCTCATCGG CCGCGGCCTT GCCCCAGTGC AGGCCTATCT GCACATCCCA
    GACATCATCA AGGTGGCCAA GGAAAACAAT GTGGATGCAG TGCACCCTGG CTACGGGTTC
    CTGTCAGAGC GAGCAGACTT TGCCCAAGCC TGCCAGGATG CAGGGGTTCG GTTTATTGGG
    CCGAGCCCTG AGGTGGTCCG CAAGATGGGC GATAAGGTGG AGGCCCGGGC CATCGCCATT
    GCTGCAGGTG TCCCTGTGGT CCCTGGCACA GATGCCCCCA TCACTTCCTT GCACGAGGCC
    CATGAATTCT CTAACACCTA TGGCTTCCCC ATTATCTTCA AGGCCGCCTA TGGAGGTGGG
    GGACGTGGCA TGAGGGTCGT GCACAGTTAT GAGGAGCTGG AGGAAAGCTA TAACCGGGCC
    TACTCGGAGG CCCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATTGAG
    AAGCCACGCC ACATCGAGGT GCAGATCCTG GGAGACCAGT ACGGGAATAT TCTGCACCTC
    TATGAGCGAG ACTGTTCCAT CCAACGGCGG CACCAGAAAG TGGTTGAGAT TGCTCCTGCA
    GCCCACCTGG ATCCCCAGCT TCGGACACGG CTCACCAGTG ACTCTGTCAA ACTTGCTAAG
    CAGGTGGGCT ACGAGAACGC AGGCACGGTG GAGTTCCTGG TGGACCGGCA TGGCAGGCAC
    TACTTCATCG AGGTCAACTC ACGCCTGCAG GTGGAGCACA CAGTCACCGA GGAGATCACA
    GATGTGGATC TGGTTCACGC CCAGATCCAT GTGGCCGAGG GCCGGAGCCT GCCTGACCTG
    GGCCTGCGGC AGGAGAATAT CCGTATCAAC GGTTGTGCCA TTCAGTGCCG GGTCACCACC
    GAGGACCCTG CACGCAGCTT CCAGCCAGAC ACTGGCCGAA TCGAGGTGTT CCGGAGCGGA
    GAGGGCATGG GCATCCGCCT GGATAATGCA TCTGCCTTCC AAGGAGCTGT CATCTCCCCG
    CACTATGACT CCCTGCTCGT CAAAGTCATT GCTCATGGCA AAGACCACCC CACGGCCGCC
    ACCAAGATGA GCAGAGCCCT TGCGGAGTTC CGAGTCCGAG GTGTAAAGAC CAACATCCCC
    TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACCCAGTTC
    ATCGATGAGA ACCCGGAGCT TTTCCAGCTG CGGCCTGCGC AGAACCGGGC TCAGAAGCTG
    CTGCACTACC TCGGTCATGT CATGGTGAAC GGACCAACCA CCCCAATCCC TGTCAAGGCC
    AGCCCCAGCC CCACGGACCC TGTTGTCCCT GCGGTGCCCA TAGGCCCACC CCCAGCTGGT
    TTCAGAGACA TCCTGCTGCG GGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GAGGAACCAC
    CAGGGGCTGC TGCTCATGGA CACAACCTTC AGGGATGCCC ACCAGTCGCT GCTGGCCACT
    CGTGTGCGAA CCCATGATCT CAAAAAGATT GCCCCCTACG TTGCCCACAA CTTCAGCAAG
    CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACGTTTGATG TTGCCATGCG CTTTCTGTAC
    GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCGTTTCAG
    ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCTGACAA TGTGGTGTTC
    AAGTTCTGTG AGGTAGCCAA GGAGAATGGC ATGGACGTCT TCCGGGTTTT TGACTCCCTC
    AACTACATGC CAAATCTGCT ACTGGGCATG GAGGCTGCAG GCAATGCTGG TGGCGTGGTG
    GAGGCTGCCA TCTCCTACAC TGGTGACGTG GCCGACCCCA ACCGCACCAA ATACTCACTG
    CAGTACTACA TGGGCTTGGC GGAAGAGCTG GTGCGAGCTG GCACCCATAT CCTGTGTATC
    AAGGACATGG CAGGGCTGCT AAAGCCTACA GCCTGCACCA TGCTGGTCAG CTCCCTCAGG
    GACCGGTTCC CCGACCTTCC ACTGCACATC CACACCCATG ACACATCAGG GGCAGGTGTG
    GCAGCTATGT TGGCCTGTGC CCAGGCTGGG GCTGATGTGG TAGATGTGGC ATCTGACTCC
    ATGTCCGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGCAC CCGAGGGACT
    CCCCTGGACA CAGGGGTGCC CCTGGAACGT GTGTTTGACT ACAGTGAGTA CTGGGAGGGG
    GCCCGGGGAT TGTATGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAATTCGGAC
    GTGTATGAGA ACGAGATCCC AGGCGGCCAG TACACCAATC TGCACTTCCA GGCACACAGC
    ATGGGGCTTG GCTCCAAGTT CAAGGAAGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG
    CTGGGCGATC TCATCAAGGT GACCCCGTCC TCCAAGATCG TGGGCGACCT GGCCCAGTTC
    ATGGTACAGA ATGGGCTGAG CCGGGCAGAG GCTGAAGCCC AGGCAGAAGA ATTGTCCTTC
    CCCCGTTCCG TGGTGGAGTT CCTGCAGGGC TACATTGGCA TTCCCCACGG GGGCTTCCCT
    GAGCCCCTTC GCTCTAAGGT GCTAAAGGAC CTGCCAAGGG TAGAAGGGCG GCCAGGAGCC
    TCCCTCCCCC CACTGGATCT GCAGCTGCTG GAGAAGGAGC TGACTGAACG GCATGGGGAG
    GAGGTGACCC CAGAAGATGT GCTCTCAGCA GCCATATATC CAGACGTCTT TGCCCACTTC
    AAGGACTTCA CAGCTACCTT TGGCCCCCTG GATAGCCTCA ATACCCGCCT CTTCCTGCAG
    GGACCCAAAA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC ACTGCACATC
    AAGGCTCTGG CCATAAGTGA CCTGAACCGG GCTGGCCAGA GGCAGGTCTT CTTTGAGCTC
    AATGGACAGC TGCGGTCCAT TCTGGTCAAG GACACTCAGG CCATGAAGGA AATGCACTTC
    CACCCCAAGG CCCTGAAGGA TGTGAAGGGC CAGATTGGGG CACCCATGCC TGGGAAGGTG
    ATAGACATCA AGGTGGCAGC TGGGGCCAAG GTGGCCAAGG GCCAGCCCCT CTGTGTGCTC
    AGTGCCATGA AGATGGAGAC TGTGGTGACT TCACCCATGG AGGGCACTGT CCGCAAGGTC
    CACGTAACCA CAGACATGAC ACTGGAGGGC GATGACCTCA TCCTGGAGAT TGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_540825.2
    CDS187..3723
    Translation

    Target ORF information:

    RefSeq Version XM_540825.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pyruvate carboxylase (PC), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_540825.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCTGAAGT TCCAAACCGT CCGTGGGGGC CTGAGGCTCC TGGGTATCCA CCGAAGCTCC 
    ACTGCCCCTG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAAGTT
    ATGGTGGCCA ACAGAGGTGA GATTGCTATC CGTGTGTTCC GGGCCTGCAC AGAGCTGGGC
    ATCCGCACTG TAGCTGTCTA CTCGGAGCAG GACACAGGCC AGATGCACCG GCAGAAAGCA
    GATGAAGCCT ACCTCATCGG CCGCGGCCTT GCCCCAGTGC AGGCCTATCT GCACATCCCA
    GACATCATCA AGGTGGCCAA GGAAAACAAT GTGGATGCAG TGCACCCTGG CTACGGGTTC
    CTGTCAGAGC GAGCAGACTT TGCCCAAGCC TGCCAGGATG CAGGGGTTCG GTTTATTGGG
    CCGAGCCCTG AGGTGGTCCG CAAGATGGGC GATAAGGTGG AGGCCCGGGC CATCGCCATT
    GCTGCAGGTG TCCCTGTGGT CCCTGGCACA GATGCCCCCA TCACTTCCTT GCACGAGGCC
    CATGAATTCT CTAACACCTA TGGCTTCCCC ATTATCTTCA AGGCCGCCTA TGGAGGTGGG
    GGACGTGGCA TGAGGGTCGT GCACAGTTAT GAGGAGCTGG AGGAAAGCTA TAACCGGGCC
    TACTCGGAGG CCCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATTGAG
    AAGCCACGCC ACATCGAGGT GCAGATCCTG GGAGACCAGT ACGGGAATAT TCTGCACCTC
    TATGAGCGAG ACTGTTCCAT CCAACGGCGG CACCAGAAAG TGGTTGAGAT TGCTCCTGCA
    GCCCACCTGG ATCCCCAGCT TCGGACACGG CTCACCAGTG ACTCTGTCAA ACTTGCTAAG
    CAGGTGGGCT ACGAGAACGC AGGCACGGTG GAGTTCCTGG TGGACCGGCA TGGCAGGCAC
    TACTTCATCG AGGTCAACTC ACGCCTGCAG GTGGAGCACA CAGTCACCGA GGAGATCACA
    GATGTGGATC TGGTTCACGC CCAGATCCAT GTGGCCGAGG GCCGGAGCCT GCCTGACCTG
    GGCCTGCGGC AGGAGAATAT CCGTATCAAC GGTTGTGCCA TTCAGTGCCG GGTCACCACC
    GAGGACCCTG CACGCAGCTT CCAGCCAGAC ACTGGCCGAA TCGAGGTGTT CCGGAGCGGA
    GAGGGCATGG GCATCCGCCT GGATAATGCA TCTGCCTTCC AAGGAGCTGT CATCTCCCCG
    CACTATGACT CCCTGCTCGT CAAAGTCATT GCTCATGGCA AAGACCACCC CACGGCCGCC
    ACCAAGATGA GCAGAGCCCT TGCGGAGTTC CGAGTCCGAG GTGTAAAGAC CAACATCCCC
    TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACCCAGTTC
    ATCGATGAGA ACCCGGAGCT TTTCCAGCTG CGGCCTGCGC AGAACCGGGC TCAGAAGCTG
    CTGCACTACC TCGGTCATGT CATGGTGAAC GGACCAACCA CCCCAATCCC TGTCAAGGCC
    AGCCCCAGCC CCACGGACCC TGTTGTCCCT GCGGTGCCCA TAGGCCCACC CCCAGCTGGT
    TTCAGAGACA TCCTGCTGCG GGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GAGGAACCAC
    CAGGGGCTGC TGCTCATGGA CACAACCTTC AGGGATGCCC ACCAGTCGCT GCTGGCCACT
    CGTGTGCGAA CCCATGATCT CAAAAAGATT GCCCCCTACG TTGCCCACAA CTTCAGCAAG
    CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACGTTTGATG TTGCCATGCG CTTTCTGTAC
    GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCGTTTCAG
    ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCTGACAA TGTGGTGTTC
    AAGTTCTGTG AGGTAGCCAA GGAGAATGGC ATGGACGTCT TCCGGGTTTT TGACTCCCTC
    AACTACATGC CAAATCTGCT ACTGGGCATG GAGGCTGCAG GCAATGCTGG TGGCGTGGTG
    GAGGCTGCCA TCTCCTACAC TGGTGACGTG GCCGACCCCA ACCGCACCAA ATACTCACTG
    CAGTACTACA TGGGCTTGGC GGAAGAGCTG GTGCGAGCTG GCACCCATAT CCTGTGTATC
    AAGGACATGG CAGGGCTGCT AAAGCCTACA GCCTGCACCA TGCTGGTCAG CTCCCTCAGG
    GACCGGTTCC CCGACCTTCC ACTGCACATC CACACCCATG ACACATCAGG GGCAGGTGTG
    GCAGCTATGT TGGCCTGTGC CCAGGCTGGG GCTGATGTGG TAGATGTGGC ATCTGACTCC
    ATGTCCGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGCAC CCGAGGGACT
    CCCCTGGACA CAGGGGTGCC CCTGGAACGT GTGTTTGACT ACAGTGAGTA CTGGGAGGGG
    GCCCGGGGAT TGTATGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAATTCGGAC
    GTGTATGAGA ACGAGATCCC AGGCGGCCAG TACACCAATC TGCACTTCCA GGCACACAGC
    ATGGGGCTTG GCTCCAAGTT CAAGGAAGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG
    CTGGGCGATC TCATCAAGGT GACCCCGTCC TCCAAGATCG TGGGCGACCT GGCCCAGTTC
    ATGGTACAGA ATGGGCTGAG CCGGGCAGAG GCTGAAGCCC AGGCAGAAGA ATTGTCCTTC
    CCCCGTTCCG TGGTGGAGTT CCTGCAGGGC TACATTGGCA TTCCCCACGG GGGCTTCCCT
    GAGCCCCTTC GCTCTAAGGT GCTAAAGGAC CTGCCAAGGG TAGAAGGGCG GCCAGGAGCC
    TCCCTCCCCC CACTGGATCT GCAGCTGCTG GAGAAGGAGC TGACTGAACG GCATGGGGAG
    GAGGTGACCC CAGAAGATGT GCTCTCAGCA GCCATATATC CAGACGTCTT TGCCCACTTC
    AAGGACTTCA CAGCTACCTT TGGCCCCCTG GATAGCCTCA ATACCCGCCT CTTCCTGCAG
    GGACCCAAAA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC ACTGCACATC
    AAGGCTCTGG CCATAAGTGA CCTGAACCGG GCTGGCCAGA GGCAGGTCTT CTTTGAGCTC
    AATGGACAGC TGCGGTCCAT TCTGGTCAAG GACACTCAGG CCATGAAGGA AATGCACTTC
    CACCCCAAGG CCCTGAAGGA TGTGAAGGGC CAGATTGGGG CACCCATGCC TGGGAAGGTG
    ATAGACATCA AGGTGGCAGC TGGGGCCAAG GTGGCCAAGG GCCAGCCCCT CTGTGTGCTC
    AGTGCCATGA AGATGGAGAC TGTGGTGACT TCACCCATGG AGGGCACTGT CCGCAAGGTC
    CACGTAACCA CAGACATGAC ACTGGAGGGC GATGACCTCA TCCTGGAGAT TGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20284
    Clone ID Related Accession (Same CDS sequence) XM_540825.6 , XM_005631416.2 , XM_005631416.3 , XM_540825.5
    Accession Version XM_005631416.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3537bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product pyruvate carboxylase, mitochondrial isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005631416.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCTGAAGT TCCAAACCGT CCGTGGGGGC CTGAGGCTCC TGGGTATCCA CCGAAGCTCC 
    ACTGCCCCTG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAAGTT
    ATGGTGGCCA ACAGAGGTGA GATTGCTATC CGTGTGTTCC GGGCCTGCAC AGAGCTGGGC
    ATCCGCACTG TAGCTGTCTA CTCGGAGCAG GACACAGGCC AGATGCACCG GCAGAAAGCA
    GATGAAGCCT ACCTCATCGG CCGCGGCCTT GCCCCAGTGC AGGCCTATCT GCACATCCCA
    GACATCATCA AGGTGGCCAA GGAAAACAAT GTGGATGCAG TGCACCCTGG CTACGGGTTC
    CTGTCAGAGC GAGCAGACTT TGCCCAAGCC TGCCAGGATG CAGGGGTTCG GTTTATTGGG
    CCGAGCCCTG AGGTGGTCCG CAAGATGGGC GATAAGGTGG AGGCCCGGGC CATCGCCATT
    GCTGCAGGTG TCCCTGTGGT CCCTGGCACA GATGCCCCCA TCACTTCCTT GCACGAGGCC
    CATGAATTCT CTAACACCTA TGGCTTCCCC ATTATCTTCA AGGCCGCCTA TGGAGGTGGG
    GGACGTGGCA TGAGGGTCGT GCACAGTTAT GAGGAGCTGG AGGAAAGCTA TAACCGGGCC
    TACTCGGAGG CCCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATTGAG
    AAGCCACGCC ACATCGAGGT GCAGATCCTG GGAGACCAGT ACGGGAATAT TCTGCACCTC
    TATGAGCGAG ACTGTTCCAT CCAACGGCGG CACCAGAAAG TGGTTGAGAT TGCTCCTGCA
    GCCCACCTGG ATCCCCAGCT TCGGACACGG CTCACCAGTG ACTCTGTCAA ACTTGCTAAG
    CAGGTGGGCT ACGAGAACGC AGGCACGGTG GAGTTCCTGG TGGACCGGCA TGGCAGGCAC
    TACTTCATCG AGGTCAACTC ACGCCTGCAG GTGGAGCACA CAGTCACCGA GGAGATCACA
    GATGTGGATC TGGTTCACGC CCAGATCCAT GTGGCCGAGG GCCGGAGCCT GCCTGACCTG
    GGCCTGCGGC AGGAGAATAT CCGTATCAAC GGTTGTGCCA TTCAGTGCCG GGTCACCACC
    GAGGACCCTG CACGCAGCTT CCAGCCAGAC ACTGGCCGAA TCGAGGTGTT CCGGAGCGGA
    GAGGGCATGG GCATCCGCCT GGATAATGCA TCTGCCTTCC AAGGAGCTGT CATCTCCCCG
    CACTATGACT CCCTGCTCGT CAAAGTCATT GCTCATGGCA AAGACCACCC CACGGCCGCC
    ACCAAGATGA GCAGAGCCCT TGCGGAGTTC CGAGTCCGAG GTGTAAAGAC CAACATCCCC
    TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACCCAGTTC
    ATCGATGAGA ACCCGGAGCT TTTCCAGCTG CGGCCTGCGC AGAACCGGGC TCAGAAGCTG
    CTGCACTACC TCGGTCATGT CATGGTGAAC GGACCAACCA CCCCAATCCC TGTCAAGGCC
    AGCCCCAGCC CCACGGACCC TGTTGTCCCT GCGGTGCCCA TAGGCCCACC CCCAGCTGGT
    TTCAGAGACA TCCTGCTGCG GGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GAGGAACCAC
    CAGGGGCTGC TGCTCATGGA CACAACCTTC AGGGATGCCC ACCAGTCGCT GCTGGCCACT
    CGTGTGCGAA CCCATGATCT CAAAAAGATT GCCCCCTACG TTGCCCACAA CTTCAGCAAG
    CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACGTTTGATG TTGCCATGCG CTTTCTGTAC
    GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCGTTTCAG
    ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCTGACAA TGTGGTGTTC
    AAGTTCTGTG AGGTAGCCAA GGAGAATGGC ATGGACGTCT TCCGGGTTTT TGACTCCCTC
    AACTACATGC CAAATCTGCT ACTGGGCATG GAGGCTGCAG GCAATGCTGG TGGCGTGGTG
    GAGGCTGCCA TCTCCTACAC TGGTGACGTG GCCGACCCCA ACCGCACCAA ATACTCACTG
    CAGTACTACA TGGGCTTGGC GGAAGAGCTG GTGCGAGCTG GCACCCATAT CCTGTGTATC
    AAGGACATGG CAGGGCTGCT AAAGCCTACA GCCTGCACCA TGCTGGTCAG CTCCCTCAGG
    GACCGGTTCC CCGACCTTCC ACTGCACATC CACACCCATG ACACATCAGG GGCAGGTGTG
    GCAGCTATGT TGGCCTGTGC CCAGGCTGGG GCTGATGTGG TAGATGTGGC ATCTGACTCC
    ATGTCCGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGCAC CCGAGGGACT
    CCCCTGGACA CAGGGGTGCC CCTGGAACGT GTGTTTGACT ACAGTGAGTA CTGGGAGGGG
    GCCCGGGGAT TGTATGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAATTCGGAC
    GTGTATGAGA ACGAGATCCC AGGCGGCCAG TACACCAATC TGCACTTCCA GGCACACAGC
    ATGGGGCTTG GCTCCAAGTT CAAGGAAGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG
    CTGGGCGATC TCATCAAGGT GACCCCGTCC TCCAAGATCG TGGGCGACCT GGCCCAGTTC
    ATGGTACAGA ATGGGCTGAG CCGGGCAGAG GCTGAAGCCC AGGCAGAAGA ATTGTCCTTC
    CCCCGTTCCG TGGTGGAGTT CCTGCAGGGC TACATTGGCA TTCCCCACGG GGGCTTCCCT
    GAGCCCCTTC GCTCTAAGGT GCTAAAGGAC CTGCCAAGGG TAGAAGGGCG GCCAGGAGCC
    TCCCTCCCCC CACTGGATCT GCAGCTGCTG GAGAAGGAGC TGACTGAACG GCATGGGGAG
    GAGGTGACCC CAGAAGATGT GCTCTCAGCA GCCATATATC CAGACGTCTT TGCCCACTTC
    AAGGACTTCA CAGCTACCTT TGGCCCCCTG GATAGCCTCA ATACCCGCCT CTTCCTGCAG
    GGACCCAAAA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC ACTGCACATC
    AAGGCTCTGG CCATAAGTGA CCTGAACCGG GCTGGCCAGA GGCAGGTCTT CTTTGAGCTC
    AATGGACAGC TGCGGTCCAT TCTGGTCAAG GACACTCAGG CCATGAAGGA AATGCACTTC
    CACCCCAAGG CCCTGAAGGA TGTGAAGGGC CAGATTGGGG CACCCATGCC TGGGAAGGTG
    ATAGACATCA AGGTGGCAGC TGGGGCCAAG GTGGCCAAGG GCCAGCCCCT CTGTGTGCTC
    AGTGCCATGA AGATGGAGAC TGTGGTGACT TCACCCATGG AGGGCACTGT CCGCAAGGTC
    CACGTAACCA CAGACATGAC ACTGGAGGGC GATGACCTCA TCCTGGAGAT TGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005631473.1
    CDS86..3622
    Translation

    Target ORF information:

    RefSeq Version XM_005631416.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris pyruvate carboxylase (PC), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005631416.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCTGAAGT TCCAAACCGT CCGTGGGGGC CTGAGGCTCC TGGGTATCCA CCGAAGCTCC 
    ACTGCCCCTG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAAGTT
    ATGGTGGCCA ACAGAGGTGA GATTGCTATC CGTGTGTTCC GGGCCTGCAC AGAGCTGGGC
    ATCCGCACTG TAGCTGTCTA CTCGGAGCAG GACACAGGCC AGATGCACCG GCAGAAAGCA
    GATGAAGCCT ACCTCATCGG CCGCGGCCTT GCCCCAGTGC AGGCCTATCT GCACATCCCA
    GACATCATCA AGGTGGCCAA GGAAAACAAT GTGGATGCAG TGCACCCTGG CTACGGGTTC
    CTGTCAGAGC GAGCAGACTT TGCCCAAGCC TGCCAGGATG CAGGGGTTCG GTTTATTGGG
    CCGAGCCCTG AGGTGGTCCG CAAGATGGGC GATAAGGTGG AGGCCCGGGC CATCGCCATT
    GCTGCAGGTG TCCCTGTGGT CCCTGGCACA GATGCCCCCA TCACTTCCTT GCACGAGGCC
    CATGAATTCT CTAACACCTA TGGCTTCCCC ATTATCTTCA AGGCCGCCTA TGGAGGTGGG
    GGACGTGGCA TGAGGGTCGT GCACAGTTAT GAGGAGCTGG AGGAAAGCTA TAACCGGGCC
    TACTCGGAGG CCCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATTGAG
    AAGCCACGCC ACATCGAGGT GCAGATCCTG GGAGACCAGT ACGGGAATAT TCTGCACCTC
    TATGAGCGAG ACTGTTCCAT CCAACGGCGG CACCAGAAAG TGGTTGAGAT TGCTCCTGCA
    GCCCACCTGG ATCCCCAGCT TCGGACACGG CTCACCAGTG ACTCTGTCAA ACTTGCTAAG
    CAGGTGGGCT ACGAGAACGC AGGCACGGTG GAGTTCCTGG TGGACCGGCA TGGCAGGCAC
    TACTTCATCG AGGTCAACTC ACGCCTGCAG GTGGAGCACA CAGTCACCGA GGAGATCACA
    GATGTGGATC TGGTTCACGC CCAGATCCAT GTGGCCGAGG GCCGGAGCCT GCCTGACCTG
    GGCCTGCGGC AGGAGAATAT CCGTATCAAC GGTTGTGCCA TTCAGTGCCG GGTCACCACC
    GAGGACCCTG CACGCAGCTT CCAGCCAGAC ACTGGCCGAA TCGAGGTGTT CCGGAGCGGA
    GAGGGCATGG GCATCCGCCT GGATAATGCA TCTGCCTTCC AAGGAGCTGT CATCTCCCCG
    CACTATGACT CCCTGCTCGT CAAAGTCATT GCTCATGGCA AAGACCACCC CACGGCCGCC
    ACCAAGATGA GCAGAGCCCT TGCGGAGTTC CGAGTCCGAG GTGTAAAGAC CAACATCCCC
    TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACCCAGTTC
    ATCGATGAGA ACCCGGAGCT TTTCCAGCTG CGGCCTGCGC AGAACCGGGC TCAGAAGCTG
    CTGCACTACC TCGGTCATGT CATGGTGAAC GGACCAACCA CCCCAATCCC TGTCAAGGCC
    AGCCCCAGCC CCACGGACCC TGTTGTCCCT GCGGTGCCCA TAGGCCCACC CCCAGCTGGT
    TTCAGAGACA TCCTGCTGCG GGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GAGGAACCAC
    CAGGGGCTGC TGCTCATGGA CACAACCTTC AGGGATGCCC ACCAGTCGCT GCTGGCCACT
    CGTGTGCGAA CCCATGATCT CAAAAAGATT GCCCCCTACG TTGCCCACAA CTTCAGCAAG
    CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACGTTTGATG TTGCCATGCG CTTTCTGTAC
    GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCGTTTCAG
    ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCTGACAA TGTGGTGTTC
    AAGTTCTGTG AGGTAGCCAA GGAGAATGGC ATGGACGTCT TCCGGGTTTT TGACTCCCTC
    AACTACATGC CAAATCTGCT ACTGGGCATG GAGGCTGCAG GCAATGCTGG TGGCGTGGTG
    GAGGCTGCCA TCTCCTACAC TGGTGACGTG GCCGACCCCA ACCGCACCAA ATACTCACTG
    CAGTACTACA TGGGCTTGGC GGAAGAGCTG GTGCGAGCTG GCACCCATAT CCTGTGTATC
    AAGGACATGG CAGGGCTGCT AAAGCCTACA GCCTGCACCA TGCTGGTCAG CTCCCTCAGG
    GACCGGTTCC CCGACCTTCC ACTGCACATC CACACCCATG ACACATCAGG GGCAGGTGTG
    GCAGCTATGT TGGCCTGTGC CCAGGCTGGG GCTGATGTGG TAGATGTGGC ATCTGACTCC
    ATGTCCGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGCAC CCGAGGGACT
    CCCCTGGACA CAGGGGTGCC CCTGGAACGT GTGTTTGACT ACAGTGAGTA CTGGGAGGGG
    GCCCGGGGAT TGTATGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAATTCGGAC
    GTGTATGAGA ACGAGATCCC AGGCGGCCAG TACACCAATC TGCACTTCCA GGCACACAGC
    ATGGGGCTTG GCTCCAAGTT CAAGGAAGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG
    CTGGGCGATC TCATCAAGGT GACCCCGTCC TCCAAGATCG TGGGCGACCT GGCCCAGTTC
    ATGGTACAGA ATGGGCTGAG CCGGGCAGAG GCTGAAGCCC AGGCAGAAGA ATTGTCCTTC
    CCCCGTTCCG TGGTGGAGTT CCTGCAGGGC TACATTGGCA TTCCCCACGG GGGCTTCCCT
    GAGCCCCTTC GCTCTAAGGT GCTAAAGGAC CTGCCAAGGG TAGAAGGGCG GCCAGGAGCC
    TCCCTCCCCC CACTGGATCT GCAGCTGCTG GAGAAGGAGC TGACTGAACG GCATGGGGAG
    GAGGTGACCC CAGAAGATGT GCTCTCAGCA GCCATATATC CAGACGTCTT TGCCCACTTC
    AAGGACTTCA CAGCTACCTT TGGCCCCCTG GATAGCCTCA ATACCCGCCT CTTCCTGCAG
    GGACCCAAAA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC ACTGCACATC
    AAGGCTCTGG CCATAAGTGA CCTGAACCGG GCTGGCCAGA GGCAGGTCTT CTTTGAGCTC
    AATGGACAGC TGCGGTCCAT TCTGGTCAAG GACACTCAGG CCATGAAGGA AATGCACTTC
    CACCCCAAGG CCCTGAAGGA TGTGAAGGGC CAGATTGGGG CACCCATGCC TGGGAAGGTG
    ATAGACATCA AGGTGGCAGC TGGGGCCAAG GTGGCCAAGG GCCAGCCCCT CTGTGTGCTC
    AGTGCCATGA AGATGGAGAC TGTGGTGACT TCACCCATGG AGGGCACTGT CCGCAAGGTC
    CACGTAACCA CAGACATGAC ACTGGAGGGC GATGACCTCA TCCTGGAGAT TGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20284
    Clone ID Related Accession (Same CDS sequence) XM_540825.6 , XM_005631416.2 , XM_005631416.3 , XM_540825.5
    Accession Version XM_005631416.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3537bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pyruvate carboxylase, mitochondrial isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005631416.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCTGAAGT TCCAAACCGT CCGTGGGGGC CTGAGGCTCC TGGGTATCCA CCGAAGCTCC 
    ACTGCCCCTG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAAGTT
    ATGGTGGCCA ACAGAGGTGA GATTGCTATC CGTGTGTTCC GGGCCTGCAC AGAGCTGGGC
    ATCCGCACTG TAGCTGTCTA CTCGGAGCAG GACACAGGCC AGATGCACCG GCAGAAAGCA
    GATGAAGCCT ACCTCATCGG CCGCGGCCTT GCCCCAGTGC AGGCCTATCT GCACATCCCA
    GACATCATCA AGGTGGCCAA GGAAAACAAT GTGGATGCAG TGCACCCTGG CTACGGGTTC
    CTGTCAGAGC GAGCAGACTT TGCCCAAGCC TGCCAGGATG CAGGGGTTCG GTTTATTGGG
    CCGAGCCCTG AGGTGGTCCG CAAGATGGGC GATAAGGTGG AGGCCCGGGC CATCGCCATT
    GCTGCAGGTG TCCCTGTGGT CCCTGGCACA GATGCCCCCA TCACTTCCTT GCACGAGGCC
    CATGAATTCT CTAACACCTA TGGCTTCCCC ATTATCTTCA AGGCCGCCTA TGGAGGTGGG
    GGACGTGGCA TGAGGGTCGT GCACAGTTAT GAGGAGCTGG AGGAAAGCTA TAACCGGGCC
    TACTCGGAGG CCCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATTGAG
    AAGCCACGCC ACATCGAGGT GCAGATCCTG GGAGACCAGT ACGGGAATAT TCTGCACCTC
    TATGAGCGAG ACTGTTCCAT CCAACGGCGG CACCAGAAAG TGGTTGAGAT TGCTCCTGCA
    GCCCACCTGG ATCCCCAGCT TCGGACACGG CTCACCAGTG ACTCTGTCAA ACTTGCTAAG
    CAGGTGGGCT ACGAGAACGC AGGCACGGTG GAGTTCCTGG TGGACCGGCA TGGCAGGCAC
    TACTTCATCG AGGTCAACTC ACGCCTGCAG GTGGAGCACA CAGTCACCGA GGAGATCACA
    GATGTGGATC TGGTTCACGC CCAGATCCAT GTGGCCGAGG GCCGGAGCCT GCCTGACCTG
    GGCCTGCGGC AGGAGAATAT CCGTATCAAC GGTTGTGCCA TTCAGTGCCG GGTCACCACC
    GAGGACCCTG CACGCAGCTT CCAGCCAGAC ACTGGCCGAA TCGAGGTGTT CCGGAGCGGA
    GAGGGCATGG GCATCCGCCT GGATAATGCA TCTGCCTTCC AAGGAGCTGT CATCTCCCCG
    CACTATGACT CCCTGCTCGT CAAAGTCATT GCTCATGGCA AAGACCACCC CACGGCCGCC
    ACCAAGATGA GCAGAGCCCT TGCGGAGTTC CGAGTCCGAG GTGTAAAGAC CAACATCCCC
    TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACCCAGTTC
    ATCGATGAGA ACCCGGAGCT TTTCCAGCTG CGGCCTGCGC AGAACCGGGC TCAGAAGCTG
    CTGCACTACC TCGGTCATGT CATGGTGAAC GGACCAACCA CCCCAATCCC TGTCAAGGCC
    AGCCCCAGCC CCACGGACCC TGTTGTCCCT GCGGTGCCCA TAGGCCCACC CCCAGCTGGT
    TTCAGAGACA TCCTGCTGCG GGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GAGGAACCAC
    CAGGGGCTGC TGCTCATGGA CACAACCTTC AGGGATGCCC ACCAGTCGCT GCTGGCCACT
    CGTGTGCGAA CCCATGATCT CAAAAAGATT GCCCCCTACG TTGCCCACAA CTTCAGCAAG
    CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACGTTTGATG TTGCCATGCG CTTTCTGTAC
    GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCGTTTCAG
    ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCTGACAA TGTGGTGTTC
    AAGTTCTGTG AGGTAGCCAA GGAGAATGGC ATGGACGTCT TCCGGGTTTT TGACTCCCTC
    AACTACATGC CAAATCTGCT ACTGGGCATG GAGGCTGCAG GCAATGCTGG TGGCGTGGTG
    GAGGCTGCCA TCTCCTACAC TGGTGACGTG GCCGACCCCA ACCGCACCAA ATACTCACTG
    CAGTACTACA TGGGCTTGGC GGAAGAGCTG GTGCGAGCTG GCACCCATAT CCTGTGTATC
    AAGGACATGG CAGGGCTGCT AAAGCCTACA GCCTGCACCA TGCTGGTCAG CTCCCTCAGG
    GACCGGTTCC CCGACCTTCC ACTGCACATC CACACCCATG ACACATCAGG GGCAGGTGTG
    GCAGCTATGT TGGCCTGTGC CCAGGCTGGG GCTGATGTGG TAGATGTGGC ATCTGACTCC
    ATGTCCGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGCAC CCGAGGGACT
    CCCCTGGACA CAGGGGTGCC CCTGGAACGT GTGTTTGACT ACAGTGAGTA CTGGGAGGGG
    GCCCGGGGAT TGTATGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAATTCGGAC
    GTGTATGAGA ACGAGATCCC AGGCGGCCAG TACACCAATC TGCACTTCCA GGCACACAGC
    ATGGGGCTTG GCTCCAAGTT CAAGGAAGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG
    CTGGGCGATC TCATCAAGGT GACCCCGTCC TCCAAGATCG TGGGCGACCT GGCCCAGTTC
    ATGGTACAGA ATGGGCTGAG CCGGGCAGAG GCTGAAGCCC AGGCAGAAGA ATTGTCCTTC
    CCCCGTTCCG TGGTGGAGTT CCTGCAGGGC TACATTGGCA TTCCCCACGG GGGCTTCCCT
    GAGCCCCTTC GCTCTAAGGT GCTAAAGGAC CTGCCAAGGG TAGAAGGGCG GCCAGGAGCC
    TCCCTCCCCC CACTGGATCT GCAGCTGCTG GAGAAGGAGC TGACTGAACG GCATGGGGAG
    GAGGTGACCC CAGAAGATGT GCTCTCAGCA GCCATATATC CAGACGTCTT TGCCCACTTC
    AAGGACTTCA CAGCTACCTT TGGCCCCCTG GATAGCCTCA ATACCCGCCT CTTCCTGCAG
    GGACCCAAAA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC ACTGCACATC
    AAGGCTCTGG CCATAAGTGA CCTGAACCGG GCTGGCCAGA GGCAGGTCTT CTTTGAGCTC
    AATGGACAGC TGCGGTCCAT TCTGGTCAAG GACACTCAGG CCATGAAGGA AATGCACTTC
    CACCCCAAGG CCCTGAAGGA TGTGAAGGGC CAGATTGGGG CACCCATGCC TGGGAAGGTG
    ATAGACATCA AGGTGGCAGC TGGGGCCAAG GTGGCCAAGG GCCAGCCCCT CTGTGTGCTC
    AGTGCCATGA AGATGGAGAC TGTGGTGACT TCACCCATGG AGGGCACTGT CCGCAAGGTC
    CACGTAACCA CAGACATGAC ACTGGAGGGC GATGACCTCA TCCTGGAGAT TGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005631473.1
    CDS144..3680
    Translation

    Target ORF information:

    RefSeq Version XM_005631416.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pyruvate carboxylase (PC), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005631416.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCTGAAGT TCCAAACCGT CCGTGGGGGC CTGAGGCTCC TGGGTATCCA CCGAAGCTCC 
    ACTGCCCCTG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAAGTT
    ATGGTGGCCA ACAGAGGTGA GATTGCTATC CGTGTGTTCC GGGCCTGCAC AGAGCTGGGC
    ATCCGCACTG TAGCTGTCTA CTCGGAGCAG GACACAGGCC AGATGCACCG GCAGAAAGCA
    GATGAAGCCT ACCTCATCGG CCGCGGCCTT GCCCCAGTGC AGGCCTATCT GCACATCCCA
    GACATCATCA AGGTGGCCAA GGAAAACAAT GTGGATGCAG TGCACCCTGG CTACGGGTTC
    CTGTCAGAGC GAGCAGACTT TGCCCAAGCC TGCCAGGATG CAGGGGTTCG GTTTATTGGG
    CCGAGCCCTG AGGTGGTCCG CAAGATGGGC GATAAGGTGG AGGCCCGGGC CATCGCCATT
    GCTGCAGGTG TCCCTGTGGT CCCTGGCACA GATGCCCCCA TCACTTCCTT GCACGAGGCC
    CATGAATTCT CTAACACCTA TGGCTTCCCC ATTATCTTCA AGGCCGCCTA TGGAGGTGGG
    GGACGTGGCA TGAGGGTCGT GCACAGTTAT GAGGAGCTGG AGGAAAGCTA TAACCGGGCC
    TACTCGGAGG CCCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATTGAG
    AAGCCACGCC ACATCGAGGT GCAGATCCTG GGAGACCAGT ACGGGAATAT TCTGCACCTC
    TATGAGCGAG ACTGTTCCAT CCAACGGCGG CACCAGAAAG TGGTTGAGAT TGCTCCTGCA
    GCCCACCTGG ATCCCCAGCT TCGGACACGG CTCACCAGTG ACTCTGTCAA ACTTGCTAAG
    CAGGTGGGCT ACGAGAACGC AGGCACGGTG GAGTTCCTGG TGGACCGGCA TGGCAGGCAC
    TACTTCATCG AGGTCAACTC ACGCCTGCAG GTGGAGCACA CAGTCACCGA GGAGATCACA
    GATGTGGATC TGGTTCACGC CCAGATCCAT GTGGCCGAGG GCCGGAGCCT GCCTGACCTG
    GGCCTGCGGC AGGAGAATAT CCGTATCAAC GGTTGTGCCA TTCAGTGCCG GGTCACCACC
    GAGGACCCTG CACGCAGCTT CCAGCCAGAC ACTGGCCGAA TCGAGGTGTT CCGGAGCGGA
    GAGGGCATGG GCATCCGCCT GGATAATGCA TCTGCCTTCC AAGGAGCTGT CATCTCCCCG
    CACTATGACT CCCTGCTCGT CAAAGTCATT GCTCATGGCA AAGACCACCC CACGGCCGCC
    ACCAAGATGA GCAGAGCCCT TGCGGAGTTC CGAGTCCGAG GTGTAAAGAC CAACATCCCC
    TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACCCAGTTC
    ATCGATGAGA ACCCGGAGCT TTTCCAGCTG CGGCCTGCGC AGAACCGGGC TCAGAAGCTG
    CTGCACTACC TCGGTCATGT CATGGTGAAC GGACCAACCA CCCCAATCCC TGTCAAGGCC
    AGCCCCAGCC CCACGGACCC TGTTGTCCCT GCGGTGCCCA TAGGCCCACC CCCAGCTGGT
    TTCAGAGACA TCCTGCTGCG GGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GAGGAACCAC
    CAGGGGCTGC TGCTCATGGA CACAACCTTC AGGGATGCCC ACCAGTCGCT GCTGGCCACT
    CGTGTGCGAA CCCATGATCT CAAAAAGATT GCCCCCTACG TTGCCCACAA CTTCAGCAAG
    CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACGTTTGATG TTGCCATGCG CTTTCTGTAC
    GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCGTTTCAG
    ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCTGACAA TGTGGTGTTC
    AAGTTCTGTG AGGTAGCCAA GGAGAATGGC ATGGACGTCT TCCGGGTTTT TGACTCCCTC
    AACTACATGC CAAATCTGCT ACTGGGCATG GAGGCTGCAG GCAATGCTGG TGGCGTGGTG
    GAGGCTGCCA TCTCCTACAC TGGTGACGTG GCCGACCCCA ACCGCACCAA ATACTCACTG
    CAGTACTACA TGGGCTTGGC GGAAGAGCTG GTGCGAGCTG GCACCCATAT CCTGTGTATC
    AAGGACATGG CAGGGCTGCT AAAGCCTACA GCCTGCACCA TGCTGGTCAG CTCCCTCAGG
    GACCGGTTCC CCGACCTTCC ACTGCACATC CACACCCATG ACACATCAGG GGCAGGTGTG
    GCAGCTATGT TGGCCTGTGC CCAGGCTGGG GCTGATGTGG TAGATGTGGC ATCTGACTCC
    ATGTCCGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGCAC CCGAGGGACT
    CCCCTGGACA CAGGGGTGCC CCTGGAACGT GTGTTTGACT ACAGTGAGTA CTGGGAGGGG
    GCCCGGGGAT TGTATGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAATTCGGAC
    GTGTATGAGA ACGAGATCCC AGGCGGCCAG TACACCAATC TGCACTTCCA GGCACACAGC
    ATGGGGCTTG GCTCCAAGTT CAAGGAAGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG
    CTGGGCGATC TCATCAAGGT GACCCCGTCC TCCAAGATCG TGGGCGACCT GGCCCAGTTC
    ATGGTACAGA ATGGGCTGAG CCGGGCAGAG GCTGAAGCCC AGGCAGAAGA ATTGTCCTTC
    CCCCGTTCCG TGGTGGAGTT CCTGCAGGGC TACATTGGCA TTCCCCACGG GGGCTTCCCT
    GAGCCCCTTC GCTCTAAGGT GCTAAAGGAC CTGCCAAGGG TAGAAGGGCG GCCAGGAGCC
    TCCCTCCCCC CACTGGATCT GCAGCTGCTG GAGAAGGAGC TGACTGAACG GCATGGGGAG
    GAGGTGACCC CAGAAGATGT GCTCTCAGCA GCCATATATC CAGACGTCTT TGCCCACTTC
    AAGGACTTCA CAGCTACCTT TGGCCCCCTG GATAGCCTCA ATACCCGCCT CTTCCTGCAG
    GGACCCAAAA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC ACTGCACATC
    AAGGCTCTGG CCATAAGTGA CCTGAACCGG GCTGGCCAGA GGCAGGTCTT CTTTGAGCTC
    AATGGACAGC TGCGGTCCAT TCTGGTCAAG GACACTCAGG CCATGAAGGA AATGCACTTC
    CACCCCAAGG CCCTGAAGGA TGTGAAGGGC CAGATTGGGG CACCCATGCC TGGGAAGGTG
    ATAGACATCA AGGTGGCAGC TGGGGCCAAG GTGGCCAAGG GCCAGCCCCT CTGTGTGCTC
    AGTGCCATGA AGATGGAGAC TGTGGTGACT TCACCCATGG AGGGCACTGT CCGCAAGGTC
    CACGTAACCA CAGACATGAC ACTGGAGGGC GATGACCTCA TCCTGGAGAT TGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20284
    Clone ID Related Accession (Same CDS sequence) XM_540825.6 , XM_005631416.2 , XM_005631416.3 , XM_540825.5
    Accession Version XM_540825.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3537bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product pyruvate carboxylase, mitochondrial isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_540825.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCTGAAGT TCCAAACCGT CCGTGGGGGC CTGAGGCTCC TGGGTATCCA CCGAAGCTCC 
    ACTGCCCCTG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAAGTT
    ATGGTGGCCA ACAGAGGTGA GATTGCTATC CGTGTGTTCC GGGCCTGCAC AGAGCTGGGC
    ATCCGCACTG TAGCTGTCTA CTCGGAGCAG GACACAGGCC AGATGCACCG GCAGAAAGCA
    GATGAAGCCT ACCTCATCGG CCGCGGCCTT GCCCCAGTGC AGGCCTATCT GCACATCCCA
    GACATCATCA AGGTGGCCAA GGAAAACAAT GTGGATGCAG TGCACCCTGG CTACGGGTTC
    CTGTCAGAGC GAGCAGACTT TGCCCAAGCC TGCCAGGATG CAGGGGTTCG GTTTATTGGG
    CCGAGCCCTG AGGTGGTCCG CAAGATGGGC GATAAGGTGG AGGCCCGGGC CATCGCCATT
    GCTGCAGGTG TCCCTGTGGT CCCTGGCACA GATGCCCCCA TCACTTCCTT GCACGAGGCC
    CATGAATTCT CTAACACCTA TGGCTTCCCC ATTATCTTCA AGGCCGCCTA TGGAGGTGGG
    GGACGTGGCA TGAGGGTCGT GCACAGTTAT GAGGAGCTGG AGGAAAGCTA TAACCGGGCC
    TACTCGGAGG CCCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATTGAG
    AAGCCACGCC ACATCGAGGT GCAGATCCTG GGAGACCAGT ACGGGAATAT TCTGCACCTC
    TATGAGCGAG ACTGTTCCAT CCAACGGCGG CACCAGAAAG TGGTTGAGAT TGCTCCTGCA
    GCCCACCTGG ATCCCCAGCT TCGGACACGG CTCACCAGTG ACTCTGTCAA ACTTGCTAAG
    CAGGTGGGCT ACGAGAACGC AGGCACGGTG GAGTTCCTGG TGGACCGGCA TGGCAGGCAC
    TACTTCATCG AGGTCAACTC ACGCCTGCAG GTGGAGCACA CAGTCACCGA GGAGATCACA
    GATGTGGATC TGGTTCACGC CCAGATCCAT GTGGCCGAGG GCCGGAGCCT GCCTGACCTG
    GGCCTGCGGC AGGAGAATAT CCGTATCAAC GGTTGTGCCA TTCAGTGCCG GGTCACCACC
    GAGGACCCTG CACGCAGCTT CCAGCCAGAC ACTGGCCGAA TCGAGGTGTT CCGGAGCGGA
    GAGGGCATGG GCATCCGCCT GGATAATGCA TCTGCCTTCC AAGGAGCTGT CATCTCCCCG
    CACTATGACT CCCTGCTCGT CAAAGTCATT GCTCATGGCA AAGACCACCC CACGGCCGCC
    ACCAAGATGA GCAGAGCCCT TGCGGAGTTC CGAGTCCGAG GTGTAAAGAC CAACATCCCC
    TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACCCAGTTC
    ATCGATGAGA ACCCGGAGCT TTTCCAGCTG CGGCCTGCGC AGAACCGGGC TCAGAAGCTG
    CTGCACTACC TCGGTCATGT CATGGTGAAC GGACCAACCA CCCCAATCCC TGTCAAGGCC
    AGCCCCAGCC CCACGGACCC TGTTGTCCCT GCGGTGCCCA TAGGCCCACC CCCAGCTGGT
    TTCAGAGACA TCCTGCTGCG GGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GAGGAACCAC
    CAGGGGCTGC TGCTCATGGA CACAACCTTC AGGGATGCCC ACCAGTCGCT GCTGGCCACT
    CGTGTGCGAA CCCATGATCT CAAAAAGATT GCCCCCTACG TTGCCCACAA CTTCAGCAAG
    CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACGTTTGATG TTGCCATGCG CTTTCTGTAC
    GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCGTTTCAG
    ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCTGACAA TGTGGTGTTC
    AAGTTCTGTG AGGTAGCCAA GGAGAATGGC ATGGACGTCT TCCGGGTTTT TGACTCCCTC
    AACTACATGC CAAATCTGCT ACTGGGCATG GAGGCTGCAG GCAATGCTGG TGGCGTGGTG
    GAGGCTGCCA TCTCCTACAC TGGTGACGTG GCCGACCCCA ACCGCACCAA ATACTCACTG
    CAGTACTACA TGGGCTTGGC GGAAGAGCTG GTGCGAGCTG GCACCCATAT CCTGTGTATC
    AAGGACATGG CAGGGCTGCT AAAGCCTACA GCCTGCACCA TGCTGGTCAG CTCCCTCAGG
    GACCGGTTCC CCGACCTTCC ACTGCACATC CACACCCATG ACACATCAGG GGCAGGTGTG
    GCAGCTATGT TGGCCTGTGC CCAGGCTGGG GCTGATGTGG TAGATGTGGC ATCTGACTCC
    ATGTCCGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGCAC CCGAGGGACT
    CCCCTGGACA CAGGGGTGCC CCTGGAACGT GTGTTTGACT ACAGTGAGTA CTGGGAGGGG
    GCCCGGGGAT TGTATGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAATTCGGAC
    GTGTATGAGA ACGAGATCCC AGGCGGCCAG TACACCAATC TGCACTTCCA GGCACACAGC
    ATGGGGCTTG GCTCCAAGTT CAAGGAAGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG
    CTGGGCGATC TCATCAAGGT GACCCCGTCC TCCAAGATCG TGGGCGACCT GGCCCAGTTC
    ATGGTACAGA ATGGGCTGAG CCGGGCAGAG GCTGAAGCCC AGGCAGAAGA ATTGTCCTTC
    CCCCGTTCCG TGGTGGAGTT CCTGCAGGGC TACATTGGCA TTCCCCACGG GGGCTTCCCT
    GAGCCCCTTC GCTCTAAGGT GCTAAAGGAC CTGCCAAGGG TAGAAGGGCG GCCAGGAGCC
    TCCCTCCCCC CACTGGATCT GCAGCTGCTG GAGAAGGAGC TGACTGAACG GCATGGGGAG
    GAGGTGACCC CAGAAGATGT GCTCTCAGCA GCCATATATC CAGACGTCTT TGCCCACTTC
    AAGGACTTCA CAGCTACCTT TGGCCCCCTG GATAGCCTCA ATACCCGCCT CTTCCTGCAG
    GGACCCAAAA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC ACTGCACATC
    AAGGCTCTGG CCATAAGTGA CCTGAACCGG GCTGGCCAGA GGCAGGTCTT CTTTGAGCTC
    AATGGACAGC TGCGGTCCAT TCTGGTCAAG GACACTCAGG CCATGAAGGA AATGCACTTC
    CACCCCAAGG CCCTGAAGGA TGTGAAGGGC CAGATTGGGG CACCCATGCC TGGGAAGGTG
    ATAGACATCA AGGTGGCAGC TGGGGCCAAG GTGGCCAAGG GCCAGCCCCT CTGTGTGCTC
    AGTGCCATGA AGATGGAGAC TGTGGTGACT TCACCCATGG AGGGCACTGT CCGCAAGGTC
    CACGTAACCA CAGACATGAC ACTGGAGGGC GATGACCTCA TCCTGGAGAT TGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_540825.2
    CDS161..3697
    Translation

    Target ORF information:

    RefSeq Version XM_540825.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris pyruvate carboxylase (PC), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_540825.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCTGAAGT TCCAAACCGT CCGTGGGGGC CTGAGGCTCC TGGGTATCCA CCGAAGCTCC 
    ACTGCCCCTG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAAGTT
    ATGGTGGCCA ACAGAGGTGA GATTGCTATC CGTGTGTTCC GGGCCTGCAC AGAGCTGGGC
    ATCCGCACTG TAGCTGTCTA CTCGGAGCAG GACACAGGCC AGATGCACCG GCAGAAAGCA
    GATGAAGCCT ACCTCATCGG CCGCGGCCTT GCCCCAGTGC AGGCCTATCT GCACATCCCA
    GACATCATCA AGGTGGCCAA GGAAAACAAT GTGGATGCAG TGCACCCTGG CTACGGGTTC
    CTGTCAGAGC GAGCAGACTT TGCCCAAGCC TGCCAGGATG CAGGGGTTCG GTTTATTGGG
    CCGAGCCCTG AGGTGGTCCG CAAGATGGGC GATAAGGTGG AGGCCCGGGC CATCGCCATT
    GCTGCAGGTG TCCCTGTGGT CCCTGGCACA GATGCCCCCA TCACTTCCTT GCACGAGGCC
    CATGAATTCT CTAACACCTA TGGCTTCCCC ATTATCTTCA AGGCCGCCTA TGGAGGTGGG
    GGACGTGGCA TGAGGGTCGT GCACAGTTAT GAGGAGCTGG AGGAAAGCTA TAACCGGGCC
    TACTCGGAGG CCCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATTGAG
    AAGCCACGCC ACATCGAGGT GCAGATCCTG GGAGACCAGT ACGGGAATAT TCTGCACCTC
    TATGAGCGAG ACTGTTCCAT CCAACGGCGG CACCAGAAAG TGGTTGAGAT TGCTCCTGCA
    GCCCACCTGG ATCCCCAGCT TCGGACACGG CTCACCAGTG ACTCTGTCAA ACTTGCTAAG
    CAGGTGGGCT ACGAGAACGC AGGCACGGTG GAGTTCCTGG TGGACCGGCA TGGCAGGCAC
    TACTTCATCG AGGTCAACTC ACGCCTGCAG GTGGAGCACA CAGTCACCGA GGAGATCACA
    GATGTGGATC TGGTTCACGC CCAGATCCAT GTGGCCGAGG GCCGGAGCCT GCCTGACCTG
    GGCCTGCGGC AGGAGAATAT CCGTATCAAC GGTTGTGCCA TTCAGTGCCG GGTCACCACC
    GAGGACCCTG CACGCAGCTT CCAGCCAGAC ACTGGCCGAA TCGAGGTGTT CCGGAGCGGA
    GAGGGCATGG GCATCCGCCT GGATAATGCA TCTGCCTTCC AAGGAGCTGT CATCTCCCCG
    CACTATGACT CCCTGCTCGT CAAAGTCATT GCTCATGGCA AAGACCACCC CACGGCCGCC
    ACCAAGATGA GCAGAGCCCT TGCGGAGTTC CGAGTCCGAG GTGTAAAGAC CAACATCCCC
    TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACCCAGTTC
    ATCGATGAGA ACCCGGAGCT TTTCCAGCTG CGGCCTGCGC AGAACCGGGC TCAGAAGCTG
    CTGCACTACC TCGGTCATGT CATGGTGAAC GGACCAACCA CCCCAATCCC TGTCAAGGCC
    AGCCCCAGCC CCACGGACCC TGTTGTCCCT GCGGTGCCCA TAGGCCCACC CCCAGCTGGT
    TTCAGAGACA TCCTGCTGCG GGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GAGGAACCAC
    CAGGGGCTGC TGCTCATGGA CACAACCTTC AGGGATGCCC ACCAGTCGCT GCTGGCCACT
    CGTGTGCGAA CCCATGATCT CAAAAAGATT GCCCCCTACG TTGCCCACAA CTTCAGCAAG
    CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACGTTTGATG TTGCCATGCG CTTTCTGTAC
    GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCGTTTCAG
    ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCTGACAA TGTGGTGTTC
    AAGTTCTGTG AGGTAGCCAA GGAGAATGGC ATGGACGTCT TCCGGGTTTT TGACTCCCTC
    AACTACATGC CAAATCTGCT ACTGGGCATG GAGGCTGCAG GCAATGCTGG TGGCGTGGTG
    GAGGCTGCCA TCTCCTACAC TGGTGACGTG GCCGACCCCA ACCGCACCAA ATACTCACTG
    CAGTACTACA TGGGCTTGGC GGAAGAGCTG GTGCGAGCTG GCACCCATAT CCTGTGTATC
    AAGGACATGG CAGGGCTGCT AAAGCCTACA GCCTGCACCA TGCTGGTCAG CTCCCTCAGG
    GACCGGTTCC CCGACCTTCC ACTGCACATC CACACCCATG ACACATCAGG GGCAGGTGTG
    GCAGCTATGT TGGCCTGTGC CCAGGCTGGG GCTGATGTGG TAGATGTGGC ATCTGACTCC
    ATGTCCGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGCAC CCGAGGGACT
    CCCCTGGACA CAGGGGTGCC CCTGGAACGT GTGTTTGACT ACAGTGAGTA CTGGGAGGGG
    GCCCGGGGAT TGTATGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAATTCGGAC
    GTGTATGAGA ACGAGATCCC AGGCGGCCAG TACACCAATC TGCACTTCCA GGCACACAGC
    ATGGGGCTTG GCTCCAAGTT CAAGGAAGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG
    CTGGGCGATC TCATCAAGGT GACCCCGTCC TCCAAGATCG TGGGCGACCT GGCCCAGTTC
    ATGGTACAGA ATGGGCTGAG CCGGGCAGAG GCTGAAGCCC AGGCAGAAGA ATTGTCCTTC
    CCCCGTTCCG TGGTGGAGTT CCTGCAGGGC TACATTGGCA TTCCCCACGG GGGCTTCCCT
    GAGCCCCTTC GCTCTAAGGT GCTAAAGGAC CTGCCAAGGG TAGAAGGGCG GCCAGGAGCC
    TCCCTCCCCC CACTGGATCT GCAGCTGCTG GAGAAGGAGC TGACTGAACG GCATGGGGAG
    GAGGTGACCC CAGAAGATGT GCTCTCAGCA GCCATATATC CAGACGTCTT TGCCCACTTC
    AAGGACTTCA CAGCTACCTT TGGCCCCCTG GATAGCCTCA ATACCCGCCT CTTCCTGCAG
    GGACCCAAAA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC ACTGCACATC
    AAGGCTCTGG CCATAAGTGA CCTGAACCGG GCTGGCCAGA GGCAGGTCTT CTTTGAGCTC
    AATGGACAGC TGCGGTCCAT TCTGGTCAAG GACACTCAGG CCATGAAGGA AATGCACTTC
    CACCCCAAGG CCCTGAAGGA TGTGAAGGGC CAGATTGGGG CACCCATGCC TGGGAAGGTG
    ATAGACATCA AGGTGGCAGC TGGGGCCAAG GTGGCCAAGG GCCAGCCCCT CTGTGTGCTC
    AGTGCCATGA AGATGGAGAC TGTGGTGACT TCACCCATGG AGGGCACTGT CCGCAAGGTC
    CACGTAACCA CAGACATGAC ACTGGAGGGC GATGACCTCA TCCTGGAGAT TGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.