GJA5 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	GJA5
Entrez Gene ID	483155
Full Name	gap junction protein alpha 5
Synonyms	Cx40
General protein information	Preferred Names gap junction protein alpha 5 Names gap junction alpha-5 protein connexin 40
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001017442.2	NP_001017442.2	gap junction alpha-5 protein

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Gja5	NP_001258557.1
Pan troglodytes (chimpanzee)	GJA5	XP_513754.2
Bos taurus (cattle)	GJA5	NP_001071490.1
Rattus norvegicus (Norway rat)	Gja5	NP_062153.1
Gallus gallus (chicken)	GJA5	NP_990835.1
Homo sapiens (human)	GJA5	NP_859054.1
Canis lupus familiaris (dog)	GJA5	NP_001017442.2
Xenopus tropicalis (tropical clawed frog)	gja5	XP_004918273.1
	GJA5	XP_001094966.1

Gene Ontology
Bibliography

Related articles in PubMed

[Association between myocardial connexin 40 and 45 expression and myocardial fibrosis in the rapid atrial pacing canine model].
Xing XQ, Xu J, Su H, Lu YW
Zhonghua xin xue guan bing za zhi39(2)176-80(2011 Feb)

Changes in connexin expression and the atrial fibrillation substrate in congestive heart failure.
Burstein B, Comtois P, Michael G, Nishida K, Villeneuve L, Yeh YH, Nattel S
Circulation research105(12)1213-22(2009 Dec)

Role of the calpain system in pulmonary vein connexin remodeling in dogs with atrial fibrillation.
Zhang W, Ma X, Zhong M, Zheng Z, Li L, Wang Z, Zhang Y
Cardiology112(1)22-30(2009)

Genetic screening of the canine connexin 40 gene in dogs with inherited cardiac conduction defects.
Lee SA, Lee JS, Lee SG, Lavulo L, Cho KO, Hyun C
Biochemical genetics46(1-2)8-17(2008 Feb)

Effects of sterile pericarditis on connexins 40 and 43 in the atria: correlation with abnormal conduction and atrial arrhythmias.
Ryu K, Li L, Khrestian CM, Matsumoto N, Sahadevan J, Ruehr ML, Van Wagoner DR, Efimov IR, Waldo AL
American journal of physiology. Heart and circulatory physiology293(2)H1231-41(2007 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Increased myocardial fibrosis is positively correlated with upregulation of myocardial Cx 40 protein expression in left atrium in rapid atrial paced canines.
Title: [Association between myocardial connexin 40 and 45 expression and myocardial fibrosis in the rapid atrial pacing canine model].

Report changes in connexin expression and the atrial fibrillation substrate in congestive heart failure.
Title: Changes in connexin expression and the atrial fibrillation substrate in congestive heart failure.

During atrial fibrillation, the calpain system of the myocardial sleeve tissue is activated and may hydrolyze Cx40 protein
Title: Role of the calpain system in pulmonary vein connexin remodeling in dogs with atrial fibrillation.

three heterozygous base changes (C384G, C402T, C837T) in the dogs screened and determined them to be synonymous mutations. These mutations, however, have recently been found in an unrelated group of normal dogs.
Title: Genetic screening of the canine connexin 40 gene in dogs with inherited cardiac conduction defects.

Altered transmural gradient of Cx40/43 contributes to vulnerability of sterile pericarditis model to induction/maintenance of atrial arrhythmias.
Title: Effects of sterile pericarditis on connexins 40 and 43 in the atria: correlation with abnormal conduction and atrial arrhythmias.

The following GJA5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GJA5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe00266	NM_001017442.2 Latest version!	Canis lupus familiaris gap junction protein alpha 5 (GJA5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GJA5 ( NM_001017442.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001017442.2 Canis lupus familiaris gap junction protein alpha 5 (GJA5), mRNA.

Price & Availability↑

CloneID	OCe00266
Clone ID Related Accession (Same CDS sequence)	NM_001017442.2
Accession Version	NM_001017442.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Canis lupus familiaris(dog)
Product	gap junction alpha-5 protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DN376037.1 and AAEX03011037.1. On Aug 10, 2010 this sequence version replaced NM_001017442.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: EF589114.1, DN388154.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN07419797 [ECO:0000348] ##Evidence-Data-END##

ATGGGTGACT GGAGCTTCCT GGGCGAGTTC CTGGAGGAAG TACACAAGCA CTCCACAGTG 
ATAGGCAAGG TCTGGCTCAC TGTCCTTTTC ATATTCCGCA TGCTGGTGCT GGGCACAGCT 
GCCGAGTCAT CCTGGGGGGA TGAGCAGGCA GATTTCCAGT GTGATACCAT GCAGCCTGGC 
TGTGGGAATG TCTGCTACGA CCAAGCCTTC CCCATCTCTC ACATCCGCTA CTGGGTGCTG 
CAGATCATCT TCGTGTCCAC ACCATCTTTG GTGTACATGG GCCACGCCAT GCACACGGTG 
CGCATGCAGG AGAAGCGGAA GCTGCGGGAG GCTGAGAGGG CCAAAGAGGC CCGGGGCGCT 
GGCTCTTACG AGTACCCAGT GGCCGAGAAG GCAGAGCTGT CCTGCTGGGA AGAAGTGAAT 
GGAAGGATTG TCCTCCAGGG CACTCTGCTC AACACCTATG TCTGCAGTAT CCTGATCCGC 
ACTACCATGG AGGTGGCCTT CATTGTGGGC CAGTACCTTC TCTATGGGAT CTTCCTGGAC 
ACACTGCATG TCTGCCGCAG GAGTCCCTGC CCCCACCCCG TCAACTGTTA TGTATCCCGG 
CCCACAGAGA AGAATGTCTT CATTGTCTTC ATGCTGGCTG TGGCTGCACT ATCCCTCTTC 
CTCAGCCTGG CTGAACTCTA CCACCTGGGC TGGAAGAAGC TCAGACAGCG CTTTGTCAAG 
TCTGGGCAGG GCATGGCTGA GTGTCAGCTT CCTGGACCCT CGGCTGGCAT AGTCCAGAAC 
TGCACACCAC CCCCTGACTT CAATCAGTGT CTGGAAAATG GCCCTGGGGG GAAATTCTTC 
AATCCCTTCA GTAACAAGAT GGCTTCCCAG CAGAACACAG ACAATCTGGC CACTGAGCAG 
GTGCAAGGCC AGGAGCCAAT TCCTGGGGAG GGTTTCATTC ATATCCGTTA TGCCCAGAAG 
CCTGAGGTAC CTAATGGAGC CTCCCCAGGT CACCGCCTTC CCCATGGCTA TCAGAGCGAC 
AAGCGCCGTC TCAGCAAGGC CAGCAGCAAG GCCAGGTCAG ATGATCTATC AGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001017442.2
CDS	97..1173
Translation	MGDWSFLGEFLEEVHKHSTVIGKVWLTVLFIFRMLVLGTAAESSWGDEQADFQCDTMQPGCGNVCYDQAFPISHIRYWVLQIIFVSTPSLVYMGHAMHTVRMQEKRKLREAERAKEARGAGSYEYPVAEKAELSCWEEVNGRIVLQGTLLNTYVCSILIRTTMEVAFIVGQYLLYGIFLDTLHVCRRSPCPHPVNCYVSRPTEKNVFIVFMLAVAALSLFLSLAELYHLGWKKLRQRFVKSGQGMAECQLPGPSAGIVQNCTPPPDFNQCLENGPGGKFFNPFSNKMASQQNTDNLATEQVQGQEPIPGEGFIHIRYAQKPEVPNGASPGHRLPHGYQSDKRRLSKASSKARSDDLSV

Target ORF information:

RefSeq Version	NM_001017442.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris gap junction protein alpha 5 (GJA5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001017442.2

ORF Insert Sequence:

ATGGGTGACT GGAGCTTCCT GGGCGAGTTC CTGGAGGAAG TACACAAGCA CTCCACAGTG 
ATAGGCAAGG TCTGGCTCAC TGTCCTTTTC ATATTCCGCA TGCTGGTGCT GGGCACAGCT 
GCCGAGTCAT CCTGGGGGGA TGAGCAGGCA GATTTCCAGT GTGATACCAT GCAGCCTGGC 
TGTGGGAATG TCTGCTACGA CCAAGCCTTC CCCATCTCTC ACATCCGCTA CTGGGTGCTG 
CAGATCATCT TCGTGTCCAC ACCATCTTTG GTGTACATGG GCCACGCCAT GCACACGGTG 
CGCATGCAGG AGAAGCGGAA GCTGCGGGAG GCTGAGAGGG CCAAAGAGGC CCGGGGCGCT 
GGCTCTTACG AGTACCCAGT GGCCGAGAAG GCAGAGCTGT CCTGCTGGGA AGAAGTGAAT 
GGAAGGATTG TCCTCCAGGG CACTCTGCTC AACACCTATG TCTGCAGTAT CCTGATCCGC 
ACTACCATGG AGGTGGCCTT CATTGTGGGC CAGTACCTTC TCTATGGGAT CTTCCTGGAC 
ACACTGCATG TCTGCCGCAG GAGTCCCTGC CCCCACCCCG TCAACTGTTA TGTATCCCGG 
CCCACAGAGA AGAATGTCTT CATTGTCTTC ATGCTGGCTG TGGCTGCACT ATCCCTCTTC 
CTCAGCCTGG CTGAACTCTA CCACCTGGGC TGGAAGAAGC TCAGACAGCG CTTTGTCAAG 
TCTGGGCAGG GCATGGCTGA GTGTCAGCTT CCTGGACCCT CGGCTGGCAT AGTCCAGAAC 
TGCACACCAC CCCCTGACTT CAATCAGTGT CTGGAAAATG GCCCTGGGGG GAAATTCTTC 
AATCCCTTCA GTAACAAGAT GGCTTCCCAG CAGAACACAG ACAATCTGGC CACTGAGCAG 
GTGCAAGGCC AGGAGCCAAT TCCTGGGGAG GGTTTCATTC ATATCCGTTA TGCCCAGAAG 
CCTGAGGTAC CTAATGGAGC CTCCCCAGGT CACCGCCTTC CCCATGGCTA TCAGAGCGAC 
AAGCGCCGTC TCAGCAAGGC CAGCAGCAAG GCCAGGTCAG ATGATCTATC AGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

[Association between myocardial connexin 40 and 45 expression and myocardial fibrosis in the rapid atrial pacing canine model].
Zhonghua xin xue guan bing za zhi39(2)176-80(2011 Feb)
Xing XQ,Xu J,Su H,Lu YW

Changes in connexin expression and the atrial fibrillation substrate in congestive heart failure.
Circulation research105(12)1213-22(2009 Dec)
Burstein B,Comtois P,Michael G,Nishida K,Villeneuve L,Yeh YH,Nattel S

Role of the calpain system in pulmonary vein connexin remodeling in dogs with atrial fibrillation.
Cardiology112(1)22-30(2009)
Zhang W,Ma X,Zhong M,Zheng Z,Li L,Wang Z,Zhang Y

Genetic screening of the canine connexin 40 gene in dogs with inherited cardiac conduction defects.
Biochemical genetics46(1-2)8-17(2008 Feb)
Lee SA,Lee JS,Lee SG,Lavulo L,Cho KO,Hyun C

Effects of sterile pericarditis on connexins 40 and 43 in the atria: correlation with abnormal conduction and atrial arrhythmias.
American journal of physiology. Heart and circulatory physiology293(2)H1231-41(2007 Aug)
Ryu K,Li L,Khrestian CM,Matsumoto N,Sahadevan J,Ruehr ML,Van Wagoner DR,Efimov IR,Waldo AL

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GJA5 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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