DYSF cDNA ORF clone, Canis lupus familiaris(dog)

The following DYSF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DYSF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe294454 XM_022404845.1
Latest version!
Canis lupus familiaris dysferlin (DYSF), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OCe294455 XM_022404846.1
Latest version!
Canis lupus familiaris dysferlin (DYSF), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OCe294456 XM_022404847.1
Latest version!
Canis lupus familiaris dysferlin (DYSF), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OCe294457 XM_022404848.1
Latest version!
Canis lupus familiaris dysferlin (DYSF), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OCe294458 XM_022404849.1
Latest version!
Canis lupus familiaris dysferlin (DYSF), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OCe294459 XM_022404850.1
Latest version!
Canis lupus familiaris dysferlin (DYSF), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OCe294460 XM_022404851.1
Latest version!
Canis lupus familiaris dysferlin (DYSF), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OCe294461 XM_022404852.1
Latest version!
Canis lupus familiaris dysferlin (DYSF), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OCe294462 XM_022404853.1
Latest version!
Canis lupus familiaris dysferlin (DYSF), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OCe294463 XM_022404854.1
Latest version!
Canis lupus familiaris dysferlin (DYSF), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OCe19206 XM_003432234.4
Latest version!
Canis lupus familiaris dysferlin (DYSF), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe19207 XM_003432229.4
Latest version!
Canis lupus familiaris dysferlin (DYSF), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe19208 XM_003432230.4
Latest version!
Canis lupus familiaris dysferlin (DYSF), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe19209 XM_003432225.4
Latest version!
Canis lupus familiaris dysferlin (DYSF), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe19210 XM_003432226.4
Latest version!
Canis lupus familiaris dysferlin (DYSF), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe19211 XM_003432222.4
Latest version!
Canis lupus familiaris dysferlin (DYSF), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe19212 XM_003432233.4
Latest version!
Canis lupus familiaris dysferlin (DYSF), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe19213 XM_003432228.4
Latest version!
Canis lupus familiaris dysferlin (DYSF), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe19214 XM_003432231.4
Latest version!
Canis lupus familiaris dysferlin (DYSF), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe19215 XM_003432224.4
Latest version!
Canis lupus familiaris dysferlin (DYSF), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe19216 XM_003432227.4
Latest version!
Canis lupus familiaris dysferlin (DYSF), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe19217 XM_540237.6
Latest version!
Canis lupus familiaris dysferlin (DYSF), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe19218 XM_003432232.4
Latest version!
Canis lupus familiaris dysferlin (DYSF), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe19219 XM_003432223.4
Latest version!
Canis lupus familiaris dysferlin (DYSF), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
View Old Accession Versions >>
OCe19219 XM_003432223.3 Canis lupus familiaris dysferlin (DYSF), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe19206 XM_003432234.3 Canis lupus familiaris dysferlin (DYSF), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe19209 XM_003432225.3 Canis lupus familiaris dysferlin (DYSF), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe19208 XM_003432230.3 Canis lupus familiaris dysferlin (DYSF), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe19213 XM_003432228.3 Canis lupus familiaris dysferlin (DYSF), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe19216 XM_003432227.3 Canis lupus familiaris dysferlin (DYSF), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe19217 XM_540237.5 Canis lupus familiaris dysferlin (DYSF), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe19210 XM_003432226.3 Canis lupus familiaris dysferlin (DYSF), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe19214 XM_003432231.3 Canis lupus familiaris dysferlin (DYSF), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe19215 XM_003432224.3 Canis lupus familiaris dysferlin (DYSF), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe19218 XM_003432232.3 Canis lupus familiaris dysferlin (DYSF), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe19207 XM_003432229.3 Canis lupus familiaris dysferlin (DYSF), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe19211 XM_003432222.3 Canis lupus familiaris dysferlin (DYSF), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe19212 XM_003432233.3 Canis lupus familiaris dysferlin (DYSF), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe294454
    Clone ID Related Accession (Same CDS sequence) XM_022404845.1
    Accession Version XM_022404845.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6465bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product dysferlin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG
    CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC
    GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG
    AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT
    CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG
    ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT
    GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT
    TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC
    GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT
    ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT
    GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC
    GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC
    AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC
    AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC
    ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG
    GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT
    GCCCCTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTGG TCAAGCCTTC TCCAGCCACG
    GACCTGGATG ACCATCTGGG CTTCCTTCCC ACCTTCGGGC CCTGCTTCGT CAATCTCTAT
    GGCAGCCCCA GGGAATTCAC TGGCTTCCCG GACCCCTACG CGGAACTCAA CATGGGCAAG
    GGGGAAGGCG TGGCCTACCG AGGTCGGCTT CTGCTCTCCC TGGAGACCAA GCTGGTGGAG
    CACAGCGGCG AGCAAAGGGT GGAGGACCTC CCTGCTGATG ACATCCTTCG GGTGGAGAAA
    TACCTCCGGA GGCGCAAGTA CTCTCTCTTT GCCGCCTTCT ACTCGGCCAC CATGCTCCAG
    GATGTCGACG ATGCCATCCA GTTTGAGGTC AGCATTGGGA ACTATGGCAA CAAGTTCGAC
    ACGACCTGTC TGCCACTGGC CTCCACCACC CAGTACAGCC GGGCAGTCTT TGATGGGTGC
    CACTACTACT ACTTGCCCTG GGGCAATGTG AAGCCTGTGG TGGTGCTGTC ATCCTACTGG
    GAAGACATCA GCCACAGAGT CGAGGCTCAG AACCAGCTGC TCAGGATCGC TGACCATCTG
    GAAGCTGGCC TGGAGCAAGT CCACTTGGCT TTAAAGGCGC AGTTCAACAC AGAGGATGTG
    GACTCCCTGT TGGCCCAGCT GATGGATGAG CTCATCTCAG ACTGCAGCCA GCCCCTGGGC
    AATGTCCAAG AGATACCCTC TGCCACCCAC CTGGACCGGC ACCTGTACAG GCTGCGCACC
    CGTCACCTAG GCCAGATTAC TGAGGCGGCC CTGGCTCTGA AACTCGGGCA TGGCAAGCTC
    CCTGCCGCCC TGGAGCAGGC TGAAGACTGG CTCCTGCGTC TCCGTGCCCT GGCTGATGAG
    CCCCAGAACA GCCTGCCCGA CATCATCATC TGGATGCTGC AGGGAAACAA GCGAGTGGCG
    TACCAGCGGG TGCCTGCGCA CGAGGTCCTC TTCTCCCGGC GGGGCGCCAG CTACTGTGGC
    AAGAACTGTG GGAAGCTGCA GACGATATTT CTGAAATATC CCATGGAGGG GGTGCCCGGA
    GCCCGGATGC CAGTACAAAT ACGGGTGAAA CTCTGGTTTG GGCTTTCTGT GGATGAGAAG
    GAGTTCAACC AGTTTGCTGA GGGAAAGCTG TCCGTCTTTG CTGAAACTTA TGAGAACCAG
    ACCAAACTGG CCCTGGTTGG GAACTGGGGC ACAACAGGCC TCACCTACCC CAAGTTTTCT
    GATGTCATGG GCAAGATCAA GCTACCCAAG GATAGCTTCC GCCCCTCAGC TGGCTGGACC
    TGGGCCGGAG ACTGGTTCGT GTGTCCAGAG AAGACCCTAC TCCATGACAT GGACGCGGGC
    CACCTGAGTT TTGTGGAGGA GGTGTTTGAG AACCAGACCC GGCTCCCTGG AGGCCAGTGG
    ATCTACATGA GTGACAACTA CACAGATGTG AATGGGGATA AGGTGCTTCC TAAGGATGAC
    ATTGAGTGCC CACTGGGCTG GAAGTGGGAA GATGAAGAGT GGTCCACGGA CCTCAATCGG
    GCCGTCGATG AGCAAGGCTG GGAGTACAGT ATCACCATCC CCCCGGATCG GAAGCCAAAG
    CACTGGGTTC CTGCTGAGAA GATGTATTAC ACGCACCGTC GGCGGCGCTG GGTCCGCCTG
    CGCAGAAGGG ACCTCAGCCA GATGGAGGCA TTGAAGAGGC ACAGACAGGC AGAGGCAGAG
    GGTGAGGGCT GGGAGTACGC CTCGCTCTTG GGCTGGAAGT TCCACCTGGA GTACCGCAAG
    ACTGATGCTT TCCGCCGCCG CCGCTGGCGC CGCCGCATGG AACCGCTGGA GAAGACGGGG
    CCTGCAGCCG TGTTTGCCCT TGAGGGGGCT CTGGGTGGTG TGGTAGATGA CAGGAGTGAA
    GATTCCGTGT CCGTGTCCAC CTTGAGCTTT GGAGTGAACA GACCCACGAT TTCCTGCATC
    TTTGATTATG GGAACCGCTA CCACCTACGC TGCTACATGT ACCAGGCTCG GGACCTGCCC
    TCGATGGACA AGGACTCTTT TTCTGATCCC TACGCCATCG TCTCCTTCCT GCACCAGAGC
    CAGAAGACAG TGGTGGCGAA GAACACTCTG AACCCCACCT GGGACCAGAC GCTTATCTTC
    TATGAGATTG AGATCTTTGG TGAGCCAGCC AGTATCGCTG AGCAGCCGCC CAGCATTGTG
    GTGGAGCTGT ATGACCACGA CACCTACGGT GCAGATGAGT TTATGGGACG CTGCATTTGT
    CAGCCCAGCC TGGAGCGGAC ACCCCGGCTG GCCTGGTTCC CACTGATTAG GGGCAGCCAG
    TCGGCGGGGG AACTGCTGGC CTCTTTTGAG CTCATCCAGA GAGAAAAGCC GGCCATTCAC
    CATATTCCTG GTTTTGAGGT ACAGGATATG TCAGGAATCC TGGAAGAGCT GTGTGCACCT
    GCCTTCTTCC TCCAGGGCCT CCTCCAAACG TCAGAGGACA TAGACCTGCC CTACCCGCCA
    CCTCAGAGGG AGGCCAACAT CTTCATGGTC CCCCAGAACA TCAAGCCTGT TCTCCAGCGT
    ACTGCCATAG AGATCCTGGC GTGGGGCCTG CGGAACATGA AGAGCTACCA GCTAGCCAGC
    ATCTCCTCCC CTAGCCTTGT GGTAGAGTGC GGGGGCCAGA CAGTGCAGTC CTGTGTCATC
    AAGAACCTCC GGAAGAACCC CAACTTCGAC ATCTGTACCC TTTTCATGGA AGTGATGCTG
    CCCAAGGAGG AGGTCTACTG TCCCTCCATC GTGATCAAGG TCATTGATAA CCGCCAGTTT
    GGCCGCCGGC CTGTGGTGGG CCAGTGTACC ATCCGTTCCC TGGAGAGCTT CATGTGTGAC
    CTGTACTCCG AGGAGAATCC GTCCCCACAG GGCGGCCCAG ATGATGTGAG TTTACTCAGT
    CCTGGGGAAG ACGTGCTCAT TGACATCGAT GACAAGGAGC CCCTCATCCC CATGCAGCTT
    GCAGATGGTC TGTCGGGCTC TGCCCCCACT AACATGGCAT CTTCTCCATC CAGTCCTCAT
    AAGGCCCTCC TGGAGGAAGA GTTCATCGAC TGGTGGAGCA AATTCTTTGC CTCCACAGGG
    GAGAGGGAAA AGTGCGGCTC ATACTTGGAG AAGGGCTTTG ACACTCTAAA GGTCTATGAC
    ACACAACTGG AGAACGTGGA GGCCTTTGAG GGCCTGTCTG ACTTTTGTAA CACCTTCAAG
    CTCTACCGGG GCAAGACTCA GGAGGAGACA GAGGACCCAT CTGTTATTGG GGAGTTTAAG
    GGCCTCTTCA AAATCTATCC CCTCCCAGAA GACCCGGCTG TTCCCATGCC CCCGAGACAA
    TTCCACCAGC TGGCTGCCCA AGGGCCTCAG GAGTGCCTGG TCCGCATCTA TATCATCCGA
    GCATTTGGCC TTCAGCCCAA GGACCCCAAT GGGAAGTGTG ATCCTTACAT CAAGATCTCC
    ATAGGGAAGA AATCAGTGAG TGACCAGGAT AACTACATTC CCTGTACTCT GGAGCCTGTG
    TTTGGAAAGA TGTTCGAGCT GACCTGCACT CTGCCTCTGG AGAAGGACCT GAAGATCACA
    CTCTATGACT ATGACCTCCT CTCCAAAGAT GAAAAGATTG GGGAGACAGT CATTGACCTG
    GAGGATAGGC TGCTGTCCAA GTTTGGGGCT CGCTGCGGAC TCCCACAGAC GTACTGTGTC
    TCTGGACCCA ACCAGTGGAG GGACCAGCTC CGCCCCTCCC AGCTTCTCCA CCTCTTCTGC
    CAGCAGCGCA GACTCAAGGC TCCTGTGTAC CTCACGGACC ACGTGGTGTT CCAGGATAAA
    GAATACAGCA TTGAAGAAAT AGAGGCTGGC CGAGTCCCGA ACCCACACCT GGGCCCAGTG
    GAAGAGCGTC TGGCCTTGCA TGTCCTCCAG CAGCAGGGCC TGGTCCCTGA GCATGTGGAG
    TCCCGGCCTC TCTACAGCCC CCTGCAGCCA GACATTGAAC AGGGGAAGCT GCAGATGTGG
    ATCGACCTAT TTCCCAAGGC CCTGGGGCGT CCCGGACCTC CCTTCAACAT CACCCCGCGG
    AGAGCCAGAA GGTTTTTCCT GCGTTGTATC ATCTGGAACA CAAAGGACGT GATCCTGGAC
    GACCTCAGCA TCACAGGGGA GAAGATGAGT GATATTTATG TGAAGGGTTG GATGGTTGGC
    TTTGAAGAGC ACAAGCAAAA GACAGATGTG CATTACCGGT CCCTGGGAGG TGAAGGCAAC
    TTTAACTGGA GGTTCATTTT CCCCTTTGAC TACCTGCCAG CCGAGCAAGT CTGCACCGTC
    TCTAAGAAGG ATGCTTTCTG GAGGCTGGAC AAGACTGAGA GCAAAATCCC AGCACGAGTG
    GTATTTCAGA TCTGGGACAA TGACAAGTTC TCCTTTGACG ACTTTCTGGG CTCCTTGCAG
    TTGGATCTCA ACCACATGCC CAAGCCTGCC AAGACAGCTG AGAAGTGCTC CTTGGACCAG
    CTGGATGACA CTTTCCACCC GGAGTGGTTT GTGTCCCTTT TTGAACAGAA AACAGTTAAG
    GGCTGGTGGC CCTGCGTAGC AGAAGAGGGC GAGAAGAAGA TCCTGGCAGG CAAGCTGGAA
    ATGACCTTGG AGATCGTAGC AGAGAGTGAG CATGAGGAGC GGCCTGCTGG CCAGGGCCGG
    GATGAACCCA ACATGAACCC CAAGCTCGAG GACCCAAGGC GCCCTGACAC CTCCTTCCTC
    TGGTTCACCT CTCCATACAA GACTATGAAG TTCATCCTGT GGCGGCGGTT CCGATGTGCC
    ATTATCCTCT TCATCATTCT CTTCATCCTG CTACTATTCT TAGGCATCTT CATCTATGCT
    TTCCCGAACT ACGCTGCCAT GAAGCTGGTG AAGCCCTTCA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022260553.1
    CDS23..6487
    Translation

    Target ORF information:

    RefSeq Version XM_022404845.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris dysferlin (DYSF), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022404845.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG
    CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC
    GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG
    AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT
    CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG
    ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT
    GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT
    TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC
    GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT
    ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT
    GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC
    GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC
    AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC
    AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC
    ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG
    GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT
    GCCCCTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTGG TCAAGCCTTC TCCAGCCACG
    GACCTGGATG ACCATCTGGG CTTCCTTCCC ACCTTCGGGC CCTGCTTCGT CAATCTCTAT
    GGCAGCCCCA GGGAATTCAC TGGCTTCCCG GACCCCTACG CGGAACTCAA CATGGGCAAG
    GGGGAAGGCG TGGCCTACCG AGGTCGGCTT CTGCTCTCCC TGGAGACCAA GCTGGTGGAG
    CACAGCGGCG AGCAAAGGGT GGAGGACCTC CCTGCTGATG ACATCCTTCG GGTGGAGAAA
    TACCTCCGGA GGCGCAAGTA CTCTCTCTTT GCCGCCTTCT ACTCGGCCAC CATGCTCCAG
    GATGTCGACG ATGCCATCCA GTTTGAGGTC AGCATTGGGA ACTATGGCAA CAAGTTCGAC
    ACGACCTGTC TGCCACTGGC CTCCACCACC CAGTACAGCC GGGCAGTCTT TGATGGGTGC
    CACTACTACT ACTTGCCCTG GGGCAATGTG AAGCCTGTGG TGGTGCTGTC ATCCTACTGG
    GAAGACATCA GCCACAGAGT CGAGGCTCAG AACCAGCTGC TCAGGATCGC TGACCATCTG
    GAAGCTGGCC TGGAGCAAGT CCACTTGGCT TTAAAGGCGC AGTTCAACAC AGAGGATGTG
    GACTCCCTGT TGGCCCAGCT GATGGATGAG CTCATCTCAG ACTGCAGCCA GCCCCTGGGC
    AATGTCCAAG AGATACCCTC TGCCACCCAC CTGGACCGGC ACCTGTACAG GCTGCGCACC
    CGTCACCTAG GCCAGATTAC TGAGGCGGCC CTGGCTCTGA AACTCGGGCA TGGCAAGCTC
    CCTGCCGCCC TGGAGCAGGC TGAAGACTGG CTCCTGCGTC TCCGTGCCCT GGCTGATGAG
    CCCCAGAACA GCCTGCCCGA CATCATCATC TGGATGCTGC AGGGAAACAA GCGAGTGGCG
    TACCAGCGGG TGCCTGCGCA CGAGGTCCTC TTCTCCCGGC GGGGCGCCAG CTACTGTGGC
    AAGAACTGTG GGAAGCTGCA GACGATATTT CTGAAATATC CCATGGAGGG GGTGCCCGGA
    GCCCGGATGC CAGTACAAAT ACGGGTGAAA CTCTGGTTTG GGCTTTCTGT GGATGAGAAG
    GAGTTCAACC AGTTTGCTGA GGGAAAGCTG TCCGTCTTTG CTGAAACTTA TGAGAACCAG
    ACCAAACTGG CCCTGGTTGG GAACTGGGGC ACAACAGGCC TCACCTACCC CAAGTTTTCT
    GATGTCATGG GCAAGATCAA GCTACCCAAG GATAGCTTCC GCCCCTCAGC TGGCTGGACC
    TGGGCCGGAG ACTGGTTCGT GTGTCCAGAG AAGACCCTAC TCCATGACAT GGACGCGGGC
    CACCTGAGTT TTGTGGAGGA GGTGTTTGAG AACCAGACCC GGCTCCCTGG AGGCCAGTGG
    ATCTACATGA GTGACAACTA CACAGATGTG AATGGGGATA AGGTGCTTCC TAAGGATGAC
    ATTGAGTGCC CACTGGGCTG GAAGTGGGAA GATGAAGAGT GGTCCACGGA CCTCAATCGG
    GCCGTCGATG AGCAAGGCTG GGAGTACAGT ATCACCATCC CCCCGGATCG GAAGCCAAAG
    CACTGGGTTC CTGCTGAGAA GATGTATTAC ACGCACCGTC GGCGGCGCTG GGTCCGCCTG
    CGCAGAAGGG ACCTCAGCCA GATGGAGGCA TTGAAGAGGC ACAGACAGGC AGAGGCAGAG
    GGTGAGGGCT GGGAGTACGC CTCGCTCTTG GGCTGGAAGT TCCACCTGGA GTACCGCAAG
    ACTGATGCTT TCCGCCGCCG CCGCTGGCGC CGCCGCATGG AACCGCTGGA GAAGACGGGG
    CCTGCAGCCG TGTTTGCCCT TGAGGGGGCT CTGGGTGGTG TGGTAGATGA CAGGAGTGAA
    GATTCCGTGT CCGTGTCCAC CTTGAGCTTT GGAGTGAACA GACCCACGAT TTCCTGCATC
    TTTGATTATG GGAACCGCTA CCACCTACGC TGCTACATGT ACCAGGCTCG GGACCTGCCC
    TCGATGGACA AGGACTCTTT TTCTGATCCC TACGCCATCG TCTCCTTCCT GCACCAGAGC
    CAGAAGACAG TGGTGGCGAA GAACACTCTG AACCCCACCT GGGACCAGAC GCTTATCTTC
    TATGAGATTG AGATCTTTGG TGAGCCAGCC AGTATCGCTG AGCAGCCGCC CAGCATTGTG
    GTGGAGCTGT ATGACCACGA CACCTACGGT GCAGATGAGT TTATGGGACG CTGCATTTGT
    CAGCCCAGCC TGGAGCGGAC ACCCCGGCTG GCCTGGTTCC CACTGATTAG GGGCAGCCAG
    TCGGCGGGGG AACTGCTGGC CTCTTTTGAG CTCATCCAGA GAGAAAAGCC GGCCATTCAC
    CATATTCCTG GTTTTGAGGT ACAGGATATG TCAGGAATCC TGGAAGAGCT GTGTGCACCT
    GCCTTCTTCC TCCAGGGCCT CCTCCAAACG TCAGAGGACA TAGACCTGCC CTACCCGCCA
    CCTCAGAGGG AGGCCAACAT CTTCATGGTC CCCCAGAACA TCAAGCCTGT TCTCCAGCGT
    ACTGCCATAG AGATCCTGGC GTGGGGCCTG CGGAACATGA AGAGCTACCA GCTAGCCAGC
    ATCTCCTCCC CTAGCCTTGT GGTAGAGTGC GGGGGCCAGA CAGTGCAGTC CTGTGTCATC
    AAGAACCTCC GGAAGAACCC CAACTTCGAC ATCTGTACCC TTTTCATGGA AGTGATGCTG
    CCCAAGGAGG AGGTCTACTG TCCCTCCATC GTGATCAAGG TCATTGATAA CCGCCAGTTT
    GGCCGCCGGC CTGTGGTGGG CCAGTGTACC ATCCGTTCCC TGGAGAGCTT CATGTGTGAC
    CTGTACTCCG AGGAGAATCC GTCCCCACAG GGCGGCCCAG ATGATGTGAG TTTACTCAGT
    CCTGGGGAAG ACGTGCTCAT TGACATCGAT GACAAGGAGC CCCTCATCCC CATGCAGCTT
    GCAGATGGTC TGTCGGGCTC TGCCCCCACT AACATGGCAT CTTCTCCATC CAGTCCTCAT
    AAGGCCCTCC TGGAGGAAGA GTTCATCGAC TGGTGGAGCA AATTCTTTGC CTCCACAGGG
    GAGAGGGAAA AGTGCGGCTC ATACTTGGAG AAGGGCTTTG ACACTCTAAA GGTCTATGAC
    ACACAACTGG AGAACGTGGA GGCCTTTGAG GGCCTGTCTG ACTTTTGTAA CACCTTCAAG
    CTCTACCGGG GCAAGACTCA GGAGGAGACA GAGGACCCAT CTGTTATTGG GGAGTTTAAG
    GGCCTCTTCA AAATCTATCC CCTCCCAGAA GACCCGGCTG TTCCCATGCC CCCGAGACAA
    TTCCACCAGC TGGCTGCCCA AGGGCCTCAG GAGTGCCTGG TCCGCATCTA TATCATCCGA
    GCATTTGGCC TTCAGCCCAA GGACCCCAAT GGGAAGTGTG ATCCTTACAT CAAGATCTCC
    ATAGGGAAGA AATCAGTGAG TGACCAGGAT AACTACATTC CCTGTACTCT GGAGCCTGTG
    TTTGGAAAGA TGTTCGAGCT GACCTGCACT CTGCCTCTGG AGAAGGACCT GAAGATCACA
    CTCTATGACT ATGACCTCCT CTCCAAAGAT GAAAAGATTG GGGAGACAGT CATTGACCTG
    GAGGATAGGC TGCTGTCCAA GTTTGGGGCT CGCTGCGGAC TCCCACAGAC GTACTGTGTC
    TCTGGACCCA ACCAGTGGAG GGACCAGCTC CGCCCCTCCC AGCTTCTCCA CCTCTTCTGC
    CAGCAGCGCA GACTCAAGGC TCCTGTGTAC CTCACGGACC ACGTGGTGTT CCAGGATAAA
    GAATACAGCA TTGAAGAAAT AGAGGCTGGC CGAGTCCCGA ACCCACACCT GGGCCCAGTG
    GAAGAGCGTC TGGCCTTGCA TGTCCTCCAG CAGCAGGGCC TGGTCCCTGA GCATGTGGAG
    TCCCGGCCTC TCTACAGCCC CCTGCAGCCA GACATTGAAC AGGGGAAGCT GCAGATGTGG
    ATCGACCTAT TTCCCAAGGC CCTGGGGCGT CCCGGACCTC CCTTCAACAT CACCCCGCGG
    AGAGCCAGAA GGTTTTTCCT GCGTTGTATC ATCTGGAACA CAAAGGACGT GATCCTGGAC
    GACCTCAGCA TCACAGGGGA GAAGATGAGT GATATTTATG TGAAGGGTTG GATGGTTGGC
    TTTGAAGAGC ACAAGCAAAA GACAGATGTG CATTACCGGT CCCTGGGAGG TGAAGGCAAC
    TTTAACTGGA GGTTCATTTT CCCCTTTGAC TACCTGCCAG CCGAGCAAGT CTGCACCGTC
    TCTAAGAAGG ATGCTTTCTG GAGGCTGGAC AAGACTGAGA GCAAAATCCC AGCACGAGTG
    GTATTTCAGA TCTGGGACAA TGACAAGTTC TCCTTTGACG ACTTTCTGGG CTCCTTGCAG
    TTGGATCTCA ACCACATGCC CAAGCCTGCC AAGACAGCTG AGAAGTGCTC CTTGGACCAG
    CTGGATGACA CTTTCCACCC GGAGTGGTTT GTGTCCCTTT TTGAACAGAA AACAGTTAAG
    GGCTGGTGGC CCTGCGTAGC AGAAGAGGGC GAGAAGAAGA TCCTGGCAGG CAAGCTGGAA
    ATGACCTTGG AGATCGTAGC AGAGAGTGAG CATGAGGAGC GGCCTGCTGG CCAGGGCCGG
    GATGAACCCA ACATGAACCC CAAGCTCGAG GACCCAAGGC GCCCTGACAC CTCCTTCCTC
    TGGTTCACCT CTCCATACAA GACTATGAAG TTCATCCTGT GGCGGCGGTT CCGATGTGCC
    ATTATCCTCT TCATCATTCT CTTCATCCTG CTACTATTCT TAGGCATCTT CATCTATGCT
    TTCCCGAACT ACGCTGCCAT GAAGCTGGTG AAGCCCTTCA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe294455
    Clone ID Related Accession (Same CDS sequence) XM_022404846.1
    Accession Version XM_022404846.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6462bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product dysferlin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC
    CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC
    TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA
    CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGGTG GGGGACAGAG CCGGGCTGAG
    ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GATACAAGAT ACTCTGGAAA GAAGTGGCTG
    GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC TGGAGACGAG
    GGAGAGCCAT TACTGGATCA GAGTGGCGTC CCAGGCCCTG GGGCTCCCAC CACCCCGAGG
    AAGCCACCTT CCCATCTGCC CCCCTACCAC CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC
    GCAGCCAGAA AGCTGCTGTC CGACAAACCG CAGGACTTCC AGATCAGAGT GCAAGTGATC
    GAGGGCCGCC AGCTGCCAGG GGTGAATATC AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG
    CAGACCAAGA GGACACGGAT CCACAAGGGA AACAGCCCAC TATTTAACGA GACTCTTTTC
    TTCAATGTGT TTGACTCTCC TTCGGAGCTG TTTGATGAGC CCATCTTTAT CACGGTCGTA
    GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC
    ATCTACAGAG AGCCCCGACA TGCTTATCTG AGGAAGTGGC TGCTACTCTC CAACCCTGAT
    GACTTCTCTG TTGGGGCCAG AGGTTACCTG AAAGCAAGCC TTTATGTGCT GGGGCCCGGA
    GATGAAGCGC CTCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT TGAAAACAAT
    CTGCTCAGGC CAACTGGCGT GGCCCTGCGA GGAGCCCACT TCTGCCTGAA GATCTTCAGA
    GCGGAGGACT TACCACAAAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC
    TTTGACAGCA ACAAGAAGAA TTTGGTAGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA
    ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACT
    CTGCCTGCCA TGTTTCCCTC CATGTGTGAA AAAATGAAGA TTCGCGTCAT GGACTGGGAC
    CGCCTTACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC
    CCTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTGGTCA AGCCTTCTCC AGCCACGGAC
    CTGGATGACC ATCTGGGCTT CCTTCCCACC TTCGGGCCCT GCTTCGTCAA TCTCTATGGC
    AGCCCCAGGG AATTCACTGG CTTCCCGGAC CCCTACGCGG AACTCAACAT GGGCAAGGGG
    GAAGGCGTGG CCTACCGAGG TCGGCTTCTG CTCTCCCTGG AGACCAAGCT GGTGGAGCAC
    AGCGGCGAGC AAAGGGTGGA GGACCTCCCT GCTGATGACA TCCTTCGGGT GGAGAAATAC
    CTCCGGAGGC GCAAGTACTC TCTCTTTGCC GCCTTCTACT CGGCCACCAT GCTCCAGGAT
    GTCGACGATG CCATCCAGTT TGAGGTCAGC ATTGGGAACT ATGGCAACAA GTTCGACACG
    ACCTGTCTGC CACTGGCCTC CACCACCCAG TACAGCCGGG CAGTCTTTGA TGGGTGCCAC
    TACTACTACT TGCCCTGGGG CAATGTGAAG CCTGTGGTGG TGCTGTCATC CTACTGGGAA
    GACATCAGCC ACAGAGTCGA GGCTCAGAAC CAGCTGCTCA GGATCGCTGA CCATCTGGAA
    GCTGGCCTGG AGCAAGTCCA CTTGGCTTTA AAGGCGCAGT TCAACACAGA GGATGTGGAC
    TCCCTGTTGG CCCAGCTGAT GGATGAGCTC ATCTCAGACT GCAGCCAGCC CCTGGGCAAT
    GTCCAAGAGA TACCCTCTGC CACCCACCTG GACCGGCACC TGTACAGGCT GCGCACCCGT
    CACCTAGGCC AGATTACTGA GGCGGCCCTG GCTCTGAAAC TCGGGCATGG CAAGCTCCCT
    GCCGCCCTGG AGCAGGCTGA AGACTGGCTC CTGCGTCTCC GTGCCCTGGC TGATGAGCCC
    CAGAACAGCC TGCCCGACAT CATCATCTGG ATGCTGCAGG GAAACAAGCG AGTGGCGTAC
    CAGCGGGTGC CTGCGCACGA GGTCCTCTTC TCCCGGCGGG GCGCCAGCTA CTGTGGCAAG
    AACTGTGGGA AGCTGCAGAC GATATTTCTG AAATATCCCA TGGAGGGGGT GCCCGGAGCC
    CGGATGCCAG TACAAATACG GGTGAAACTC TGGTTTGGGC TTTCTGTGGA TGAGAAGGAG
    TTCAACCAGT TTGCTGAGGG AAAGCTGTCC GTCTTTGCTG AAACTTATGA GAACCAGACC
    AAACTGGCCC TGGTTGGGAA CTGGGGCACA ACAGGCCTCA CCTACCCCAA GTTTTCTGAT
    GTCATGGGCA AGATCAAGCT ACCCAAGGAT AGCTTCCGCC CCTCAGCTGG CTGGACCTGG
    GCCGGAGACT GGTTCGTGTG TCCAGAGAAG ACCCTACTCC ATGACATGGA CGCGGGCCAC
    CTGAGTTTTG TGGAGGAGGT GTTTGAGAAC CAGACCCGGC TCCCTGGAGG CCAGTGGATC
    TACATGAGTG ACAACTACAC AGATGTGAAT GGGGATAAGG TGCTTCCTAA GGATGACATT
    GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT GAAGAGTGGT CCACGGACCT CAATCGGGCC
    GTCGATGAGC AAGGCTGGGA GTACAGTATC ACCATCCCCC CGGATCGGAA GCCAAAGCAC
    TGGGTTCCTG CTGAGAAGAT GTATTACACG CACCGTCGGC GGCGCTGGGT CCGCCTGCGC
    AGAAGGGACC TCAGCCAGAT GGAGGCATTG AAGAGGCACA GACAGGCAGA GGCAGAGGGT
    GAGGGCTGGG AGTACGCCTC GCTCTTGGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACT
    GATGCTTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAAC CGCTGGAGAA GACGGGGCCT
    GCAGCCGTGT TTGCCCTTGA GGGGGCTCTG GGTGGTGTGG TAGATGACAG GAGTGAAGAT
    TCCGTGTCCG TGTCCACCTT GAGCTTTGGA GTGAACAGAC CCACGATTTC CTGCATCTTT
    GATTATGGGA ACCGCTACCA CCTACGCTGC TACATGTACC AGGCTCGGGA CCTGCCCTCG
    ATGGACAAGG ACTCTTTTTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA CCAGAGCCAG
    AAGACAGTGG TGGCGAAGAA CACTCTGAAC CCCACCTGGG ACCAGACGCT TATCTTCTAT
    GAGATTGAGA TCTTTGGTGA GCCAGCCAGT ATCGCTGAGC AGCCGCCCAG CATTGTGGTG
    GAGCTGTATG ACCACGACAC CTACGGTGCA GATGAGTTTA TGGGACGCTG CATTTGTCAG
    CCCAGCCTGG AGCGGACACC CCGGCTGGCC TGGTTCCCAC TGATTAGGGG CAGCCAGTCG
    GCGGGGGAAC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AAAAGCCGGC CATTCACCAT
    ATTCCTGGTT TTGAGGTACA GGATATGTCA GGAATCCTGG AAGAGCTGTG TGCACCTGCC
    TTCTTCCTCC AGGGCCTCCT CCAAACGTCA GAGGACATAG ACCTGCCCTA CCCGCCACCT
    CAGAGGGAGG CCAACATCTT CATGGTCCCC CAGAACATCA AGCCTGTTCT CCAGCGTACT
    GCCATAGAGA TCCTGGCGTG GGGCCTGCGG AACATGAAGA GCTACCAGCT AGCCAGCATC
    TCCTCCCCTA GCCTTGTGGT AGAGTGCGGG GGCCAGACAG TGCAGTCCTG TGTCATCAAG
    AACCTCCGGA AGAACCCCAA CTTCGACATC TGTACCCTTT TCATGGAAGT GATGCTGCCC
    AAGGAGGAGG TCTACTGTCC CTCCATCGTG ATCAAGGTCA TTGATAACCG CCAGTTTGGC
    CGCCGGCCTG TGGTGGGCCA GTGTACCATC CGTTCCCTGG AGAGCTTCAT GTGTGACCTG
    TACTCCGAGG AGAATCCGTC CCCACAGGGC GGCCCAGATG ATGTGAGTTT ACTCAGTCCT
    GGGGAAGACG TGCTCATTGA CATCGATGAC AAGGAGCCCC TCATCCCCAT GCAGCTTGCA
    GATGGTCTGT CGGGCTCTGC CCCCACTAAC ATGGCATCTT CTCCATCCAG TCCTCATAAG
    GCCCTCCTGG AGGAAGAGTT CATCGACTGG TGGAGCAAAT TCTTTGCCTC CACAGGGGAG
    AGGGAAAAGT GCGGCTCATA CTTGGAGAAG GGCTTTGACA CTCTAAAGGT CTATGACACA
    CAACTGGAGA ACGTGGAGGC CTTTGAGGGC CTGTCTGACT TTTGTAACAC CTTCAAGCTC
    TACCGGGGCA AGACTCAGGA GGAGACAGAG GACCCATCTG TTATTGGGGA GTTTAAGGGC
    CTCTTCAAAA TCTATCCCCT CCCAGAAGAC CCGGCTGTTC CCATGCCCCC GAGACAATTC
    CACCAGCTGG CTGCCCAAGG GCCTCAGGAG TGCCTGGTCC GCATCTATAT CATCCGAGCA
    TTTGGCCTTC AGCCCAAGGA CCCCAATGGG AAGTGTGATC CTTACATCAA GATCTCCATA
    GGGAAGAAAT CAGTGAGTGA CCAGGATAAC TACATTCCCT GTACTCTGGA GCCTGTGTTT
    GGAAAGATGT TCGAGCTGAC CTGCACTCTG CCTCTGGAGA AGGACCTGAA GATCACACTC
    TATGACTATG ACCTCCTCTC CAAAGATGAA AAGATTGGGG AGACAGTCAT TGACCTGGAG
    GATAGGCTGC TGTCCAAGTT TGGGGCTCGC TGCGGACTCC CACAGACGTA CTGTGTCTCT
    GGACCCAACC AGTGGAGGGA CCAGCTCCGC CCCTCCCAGC TTCTCCACCT CTTCTGCCAG
    CAGCGCAGAC TCAAGGCTCC TGTGTACCTC ACGGACCACG TGGTGTTCCA GGATAAAGAA
    TACAGCATTG AAGAAATAGA GGCTGGCCGA GTCCCGAACC CACACCTGGG CCCAGTGGAA
    GAGCGTCTGG CCTTGCATGT CCTCCAGCAG CAGGGCCTGG TCCCTGAGCA TGTGGAGTCC
    CGGCCTCTCT ACAGCCCCCT GCAGCCAGAC ATTGAACAGG GGAAGCTGCA GATGTGGATC
    GACCTATTTC CCAAGGCCCT GGGGCGTCCC GGACCTCCCT TCAACATCAC CCCGCGGAGA
    GCCAGAAGGT TTTTCCTGCG TTGTATCATC TGGAACACAA AGGACGTGAT CCTGGACGAC
    CTCAGCATCA CAGGGGAGAA GATGAGTGAT ATTTATGTGA AGGGTTGGAT GGTTGGCTTT
    GAAGAGCACA AGCAAAAGAC AGATGTGCAT TACCGGTCCC TGGGAGGTGA AGGCAACTTT
    AACTGGAGGT TCATTTTCCC CTTTGACTAC CTGCCAGCCG AGCAAGTCTG CACCGTCTCT
    AAGAAGGATG CTTTCTGGAG GCTGGACAAG ACTGAGAGCA AAATCCCAGC ACGAGTGGTA
    TTTCAGATCT GGGACAATGA CAAGTTCTCC TTTGACGACT TTCTGGGCTC CTTGCAGTTG
    GATCTCAACC ACATGCCCAA GCCTGCCAAG ACAGCTGAGA AGTGCTCCTT GGACCAGCTG
    GATGACACTT TCCACCCGGA GTGGTTTGTG TCCCTTTTTG AACAGAAAAC AGTTAAGGGC
    TGGTGGCCCT GCGTAGCAGA AGAGGGCGAG AAGAAGATCC TGGCAGGCAA GCTGGAAATG
    ACCTTGGAGA TCGTAGCAGA GAGTGAGCAT GAGGAGCGGC CTGCTGGCCA GGGCCGGGAT
    GAACCCAACA TGAACCCCAA GCTCGAGGAC CCAAGGCGCC CTGACACCTC CTTCCTCTGG
    TTCACCTCTC CATACAAGAC TATGAAGTTC ATCCTGTGGC GGCGGTTCCG ATGTGCCATT
    ATCCTCTTCA TCATTCTCTT CATCCTGCTA CTATTCTTAG GCATCTTCAT CTATGCTTTC
    CCGAACTACG CTGCCATGAA GCTGGTGAAG CCCTTCAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022260554.1
    CDS406..6867
    Translation

    Target ORF information:

    RefSeq Version XM_022404846.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris dysferlin (DYSF), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022404846.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC
    CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC
    TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA
    CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGGTG GGGGACAGAG CCGGGCTGAG
    ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GATACAAGAT ACTCTGGAAA GAAGTGGCTG
    GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC TGGAGACGAG
    GGAGAGCCAT TACTGGATCA GAGTGGCGTC CCAGGCCCTG GGGCTCCCAC CACCCCGAGG
    AAGCCACCTT CCCATCTGCC CCCCTACCAC CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC
    GCAGCCAGAA AGCTGCTGTC CGACAAACCG CAGGACTTCC AGATCAGAGT GCAAGTGATC
    GAGGGCCGCC AGCTGCCAGG GGTGAATATC AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG
    CAGACCAAGA GGACACGGAT CCACAAGGGA AACAGCCCAC TATTTAACGA GACTCTTTTC
    TTCAATGTGT TTGACTCTCC TTCGGAGCTG TTTGATGAGC CCATCTTTAT CACGGTCGTA
    GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC
    ATCTACAGAG AGCCCCGACA TGCTTATCTG AGGAAGTGGC TGCTACTCTC CAACCCTGAT
    GACTTCTCTG TTGGGGCCAG AGGTTACCTG AAAGCAAGCC TTTATGTGCT GGGGCCCGGA
    GATGAAGCGC CTCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT TGAAAACAAT
    CTGCTCAGGC CAACTGGCGT GGCCCTGCGA GGAGCCCACT TCTGCCTGAA GATCTTCAGA
    GCGGAGGACT TACCACAAAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC
    TTTGACAGCA ACAAGAAGAA TTTGGTAGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA
    ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACT
    CTGCCTGCCA TGTTTCCCTC CATGTGTGAA AAAATGAAGA TTCGCGTCAT GGACTGGGAC
    CGCCTTACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC
    CCTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTGGTCA AGCCTTCTCC AGCCACGGAC
    CTGGATGACC ATCTGGGCTT CCTTCCCACC TTCGGGCCCT GCTTCGTCAA TCTCTATGGC
    AGCCCCAGGG AATTCACTGG CTTCCCGGAC CCCTACGCGG AACTCAACAT GGGCAAGGGG
    GAAGGCGTGG CCTACCGAGG TCGGCTTCTG CTCTCCCTGG AGACCAAGCT GGTGGAGCAC
    AGCGGCGAGC AAAGGGTGGA GGACCTCCCT GCTGATGACA TCCTTCGGGT GGAGAAATAC
    CTCCGGAGGC GCAAGTACTC TCTCTTTGCC GCCTTCTACT CGGCCACCAT GCTCCAGGAT
    GTCGACGATG CCATCCAGTT TGAGGTCAGC ATTGGGAACT ATGGCAACAA GTTCGACACG
    ACCTGTCTGC CACTGGCCTC CACCACCCAG TACAGCCGGG CAGTCTTTGA TGGGTGCCAC
    TACTACTACT TGCCCTGGGG CAATGTGAAG CCTGTGGTGG TGCTGTCATC CTACTGGGAA
    GACATCAGCC ACAGAGTCGA GGCTCAGAAC CAGCTGCTCA GGATCGCTGA CCATCTGGAA
    GCTGGCCTGG AGCAAGTCCA CTTGGCTTTA AAGGCGCAGT TCAACACAGA GGATGTGGAC
    TCCCTGTTGG CCCAGCTGAT GGATGAGCTC ATCTCAGACT GCAGCCAGCC CCTGGGCAAT
    GTCCAAGAGA TACCCTCTGC CACCCACCTG GACCGGCACC TGTACAGGCT GCGCACCCGT
    CACCTAGGCC AGATTACTGA GGCGGCCCTG GCTCTGAAAC TCGGGCATGG CAAGCTCCCT
    GCCGCCCTGG AGCAGGCTGA AGACTGGCTC CTGCGTCTCC GTGCCCTGGC TGATGAGCCC
    CAGAACAGCC TGCCCGACAT CATCATCTGG ATGCTGCAGG GAAACAAGCG AGTGGCGTAC
    CAGCGGGTGC CTGCGCACGA GGTCCTCTTC TCCCGGCGGG GCGCCAGCTA CTGTGGCAAG
    AACTGTGGGA AGCTGCAGAC GATATTTCTG AAATATCCCA TGGAGGGGGT GCCCGGAGCC
    CGGATGCCAG TACAAATACG GGTGAAACTC TGGTTTGGGC TTTCTGTGGA TGAGAAGGAG
    TTCAACCAGT TTGCTGAGGG AAAGCTGTCC GTCTTTGCTG AAACTTATGA GAACCAGACC
    AAACTGGCCC TGGTTGGGAA CTGGGGCACA ACAGGCCTCA CCTACCCCAA GTTTTCTGAT
    GTCATGGGCA AGATCAAGCT ACCCAAGGAT AGCTTCCGCC CCTCAGCTGG CTGGACCTGG
    GCCGGAGACT GGTTCGTGTG TCCAGAGAAG ACCCTACTCC ATGACATGGA CGCGGGCCAC
    CTGAGTTTTG TGGAGGAGGT GTTTGAGAAC CAGACCCGGC TCCCTGGAGG CCAGTGGATC
    TACATGAGTG ACAACTACAC AGATGTGAAT GGGGATAAGG TGCTTCCTAA GGATGACATT
    GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT GAAGAGTGGT CCACGGACCT CAATCGGGCC
    GTCGATGAGC AAGGCTGGGA GTACAGTATC ACCATCCCCC CGGATCGGAA GCCAAAGCAC
    TGGGTTCCTG CTGAGAAGAT GTATTACACG CACCGTCGGC GGCGCTGGGT CCGCCTGCGC
    AGAAGGGACC TCAGCCAGAT GGAGGCATTG AAGAGGCACA GACAGGCAGA GGCAGAGGGT
    GAGGGCTGGG AGTACGCCTC GCTCTTGGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACT
    GATGCTTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAAC CGCTGGAGAA GACGGGGCCT
    GCAGCCGTGT TTGCCCTTGA GGGGGCTCTG GGTGGTGTGG TAGATGACAG GAGTGAAGAT
    TCCGTGTCCG TGTCCACCTT GAGCTTTGGA GTGAACAGAC CCACGATTTC CTGCATCTTT
    GATTATGGGA ACCGCTACCA CCTACGCTGC TACATGTACC AGGCTCGGGA CCTGCCCTCG
    ATGGACAAGG ACTCTTTTTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA CCAGAGCCAG
    AAGACAGTGG TGGCGAAGAA CACTCTGAAC CCCACCTGGG ACCAGACGCT TATCTTCTAT
    GAGATTGAGA TCTTTGGTGA GCCAGCCAGT ATCGCTGAGC AGCCGCCCAG CATTGTGGTG
    GAGCTGTATG ACCACGACAC CTACGGTGCA GATGAGTTTA TGGGACGCTG CATTTGTCAG
    CCCAGCCTGG AGCGGACACC CCGGCTGGCC TGGTTCCCAC TGATTAGGGG CAGCCAGTCG
    GCGGGGGAAC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AAAAGCCGGC CATTCACCAT
    ATTCCTGGTT TTGAGGTACA GGATATGTCA GGAATCCTGG AAGAGCTGTG TGCACCTGCC
    TTCTTCCTCC AGGGCCTCCT CCAAACGTCA GAGGACATAG ACCTGCCCTA CCCGCCACCT
    CAGAGGGAGG CCAACATCTT CATGGTCCCC CAGAACATCA AGCCTGTTCT CCAGCGTACT
    GCCATAGAGA TCCTGGCGTG GGGCCTGCGG AACATGAAGA GCTACCAGCT AGCCAGCATC
    TCCTCCCCTA GCCTTGTGGT AGAGTGCGGG GGCCAGACAG TGCAGTCCTG TGTCATCAAG
    AACCTCCGGA AGAACCCCAA CTTCGACATC TGTACCCTTT TCATGGAAGT GATGCTGCCC
    AAGGAGGAGG TCTACTGTCC CTCCATCGTG ATCAAGGTCA TTGATAACCG CCAGTTTGGC
    CGCCGGCCTG TGGTGGGCCA GTGTACCATC CGTTCCCTGG AGAGCTTCAT GTGTGACCTG
    TACTCCGAGG AGAATCCGTC CCCACAGGGC GGCCCAGATG ATGTGAGTTT ACTCAGTCCT
    GGGGAAGACG TGCTCATTGA CATCGATGAC AAGGAGCCCC TCATCCCCAT GCAGCTTGCA
    GATGGTCTGT CGGGCTCTGC CCCCACTAAC ATGGCATCTT CTCCATCCAG TCCTCATAAG
    GCCCTCCTGG AGGAAGAGTT CATCGACTGG TGGAGCAAAT TCTTTGCCTC CACAGGGGAG
    AGGGAAAAGT GCGGCTCATA CTTGGAGAAG GGCTTTGACA CTCTAAAGGT CTATGACACA
    CAACTGGAGA ACGTGGAGGC CTTTGAGGGC CTGTCTGACT TTTGTAACAC CTTCAAGCTC
    TACCGGGGCA AGACTCAGGA GGAGACAGAG GACCCATCTG TTATTGGGGA GTTTAAGGGC
    CTCTTCAAAA TCTATCCCCT CCCAGAAGAC CCGGCTGTTC CCATGCCCCC GAGACAATTC
    CACCAGCTGG CTGCCCAAGG GCCTCAGGAG TGCCTGGTCC GCATCTATAT CATCCGAGCA
    TTTGGCCTTC AGCCCAAGGA CCCCAATGGG AAGTGTGATC CTTACATCAA GATCTCCATA
    GGGAAGAAAT CAGTGAGTGA CCAGGATAAC TACATTCCCT GTACTCTGGA GCCTGTGTTT
    GGAAAGATGT TCGAGCTGAC CTGCACTCTG CCTCTGGAGA AGGACCTGAA GATCACACTC
    TATGACTATG ACCTCCTCTC CAAAGATGAA AAGATTGGGG AGACAGTCAT TGACCTGGAG
    GATAGGCTGC TGTCCAAGTT TGGGGCTCGC TGCGGACTCC CACAGACGTA CTGTGTCTCT
    GGACCCAACC AGTGGAGGGA CCAGCTCCGC CCCTCCCAGC TTCTCCACCT CTTCTGCCAG
    CAGCGCAGAC TCAAGGCTCC TGTGTACCTC ACGGACCACG TGGTGTTCCA GGATAAAGAA
    TACAGCATTG AAGAAATAGA GGCTGGCCGA GTCCCGAACC CACACCTGGG CCCAGTGGAA
    GAGCGTCTGG CCTTGCATGT CCTCCAGCAG CAGGGCCTGG TCCCTGAGCA TGTGGAGTCC
    CGGCCTCTCT ACAGCCCCCT GCAGCCAGAC ATTGAACAGG GGAAGCTGCA GATGTGGATC
    GACCTATTTC CCAAGGCCCT GGGGCGTCCC GGACCTCCCT TCAACATCAC CCCGCGGAGA
    GCCAGAAGGT TTTTCCTGCG TTGTATCATC TGGAACACAA AGGACGTGAT CCTGGACGAC
    CTCAGCATCA CAGGGGAGAA GATGAGTGAT ATTTATGTGA AGGGTTGGAT GGTTGGCTTT
    GAAGAGCACA AGCAAAAGAC AGATGTGCAT TACCGGTCCC TGGGAGGTGA AGGCAACTTT
    AACTGGAGGT TCATTTTCCC CTTTGACTAC CTGCCAGCCG AGCAAGTCTG CACCGTCTCT
    AAGAAGGATG CTTTCTGGAG GCTGGACAAG ACTGAGAGCA AAATCCCAGC ACGAGTGGTA
    TTTCAGATCT GGGACAATGA CAAGTTCTCC TTTGACGACT TTCTGGGCTC CTTGCAGTTG
    GATCTCAACC ACATGCCCAA GCCTGCCAAG ACAGCTGAGA AGTGCTCCTT GGACCAGCTG
    GATGACACTT TCCACCCGGA GTGGTTTGTG TCCCTTTTTG AACAGAAAAC AGTTAAGGGC
    TGGTGGCCCT GCGTAGCAGA AGAGGGCGAG AAGAAGATCC TGGCAGGCAA GCTGGAAATG
    ACCTTGGAGA TCGTAGCAGA GAGTGAGCAT GAGGAGCGGC CTGCTGGCCA GGGCCGGGAT
    GAACCCAACA TGAACCCCAA GCTCGAGGAC CCAAGGCGCC CTGACACCTC CTTCCTCTGG
    TTCACCTCTC CATACAAGAC TATGAAGTTC ATCCTGTGGC GGCGGTTCCG ATGTGCCATT
    ATCCTCTTCA TCATTCTCTT CATCCTGCTA CTATTCTTAG GCATCTTCAT CTATGCTTTC
    CCGAACTACG CTGCCATGAA GCTGGTGAAG CCCTTCAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe294456
    Clone ID Related Accession (Same CDS sequence) XM_022404847.1
    Accession Version XM_022404847.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6459bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product dysferlin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG
    CTGGCCCCCA CGGGAGGAGA GGAGGACACT GAGGACCAGG GGCTCACTGG AGACGAGGGA
    GAGCCATTAC TGGATCAGAG TGGCGTCCCA GGCCCTGGGG CTCCCACCAC CCCGAGGAAG
    CCACCTTCCC ATCTGCCCCC CTACCACCCT GGGAGCAAGA GGAAAAGAAG TGCTCCCGCA
    GCCAGAAAGC TGCTGTCCGA CAAACCGCAG GACTTCCAGA TCAGAGTGCA AGTGATCGAG
    GGCCGCCAGC TGCCAGGGGT GAATATCAAG CCTGTGATCA AGGTCACTGC TGCTGGGCAG
    ACCAAGAGGA CACGGATCCA CAAGGGAAAC AGCCCACTAT TTAACGAGAC TCTTTTCTTC
    AATGTGTTTG ACTCTCCTTC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTCGTAGAC
    TCCCGTTCTC TCAGGACAGA TGCTCTCATT GGGGAATTCC GGATGGATGT GGGTACCATC
    TACAGAGAGC CCCGACATGC TTATCTGAGG AAGTGGCTGC TACTCTCCAA CCCTGATGAC
    TTCTCTGTTG GGGCCAGAGG TTACCTGAAA GCAAGCCTTT ATGTGCTGGG GCCCGGAGAT
    GAAGCGCCTC TGGAGAGAAA GGACCCCTCT GAAGACAAGG AGGACATTGA AAACAATCTG
    CTCAGGCCAA CTGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGAT CTTCAGAGCG
    GAGGACTTAC CACAAATGGA TGATGCCGTG ATGGACAATG TGAAGCAGAT CTTTGGCTTT
    GACAGCAACA AGAAGAATTT GGTAGATCCC TTCATGGAAG TCAGCTTTGC GGGGAAAATG
    CTCTGCAGCA AGATCCTGGA GAAGACAGCC AACCCTCAGT GGAACCAGAG CATCACTCTG
    CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAAGATTC GCGTCATGGA CTGGGACCGC
    CTTACCCACA ATGACATCGT GGCCACCACC TACCTGAGTA TGTCGAAAAT CTCTGCCCCT
    GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GTGGTCAAGC CTTCTCCAGC CACGGACCTG
    GATGACCATC TGGGCTTCCT TCCCACCTTC GGGCCCTGCT TCGTCAATCT CTATGGCAGC
    CCCAGGGAAT TCACTGGCTT CCCGGACCCC TACGCGGAAC TCAACATGGG CAAGGGGGAA
    GGCGTGGCCT ACCGAGGTCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGC
    GGCGAGCAAA GGGTGGAGGA CCTCCCTGCT GATGACATCC TTCGGGTGGA GAAATACCTC
    CGGAGGCGCA AGTACTCTCT CTTTGCCGCC TTCTACTCGG CCACCATGCT CCAGGATGTC
    GACGATGCCA TCCAGTTTGA GGTCAGCATT GGGAACTATG GCAACAAGTT CGACACGACC
    TGTCTGCCAC TGGCCTCCAC CACCCAGTAC AGCCGGGCAG TCTTTGATGG GTGCCACTAC
    TACTACTTGC CCTGGGGCAA TGTGAAGCCT GTGGTGGTGC TGTCATCCTA CTGGGAAGAC
    ATCAGCCACA GAGTCGAGGC TCAGAACCAG CTGCTCAGGA TCGCTGACCA TCTGGAAGCT
    GGCCTGGAGC AAGTCCACTT GGCTTTAAAG GCGCAGTTCA ACACAGAGGA TGTGGACTCC
    CTGTTGGCCC AGCTGATGGA TGAGCTCATC TCAGACTGCA GCCAGCCCCT GGGCAATGTC
    CAAGAGATAC CCTCTGCCAC CCACCTGGAC CGGCACCTGT ACAGGCTGCG CACCCGTCAC
    CTAGGCCAGA TTACTGAGGC GGCCCTGGCT CTGAAACTCG GGCATGGCAA GCTCCCTGCC
    GCCCTGGAGC AGGCTGAAGA CTGGCTCCTG CGTCTCCGTG CCCTGGCTGA TGAGCCCCAG
    AACAGCCTGC CCGACATCAT CATCTGGATG CTGCAGGGAA ACAAGCGAGT GGCGTACCAG
    CGGGTGCCTG CGCACGAGGT CCTCTTCTCC CGGCGGGGCG CCAGCTACTG TGGCAAGAAC
    TGTGGGAAGC TGCAGACGAT ATTTCTGAAA TATCCCATGG AGGGGGTGCC CGGAGCCCGG
    ATGCCAGTAC AAATACGGGT GAAACTCTGG TTTGGGCTTT CTGTGGATGA GAAGGAGTTC
    AACCAGTTTG CTGAGGGAAA GCTGTCCGTC TTTGCTGAAA CTTATGAGAA CCAGACCAAA
    CTGGCCCTGG TTGGGAACTG GGGCACAACA GGCCTCACCT ACCCCAAGTT TTCTGATGTC
    ATGGGCAAGA TCAAGCTACC CAAGGATAGC TTCCGCCCCT CAGCTGGCTG GACCTGGGCC
    GGAGACTGGT TCGTGTGTCC AGAGAAGACC CTACTCCATG ACATGGACGC GGGCCACCTG
    AGTTTTGTGG AGGAGGTGTT TGAGAACCAG ACCCGGCTCC CTGGAGGCCA GTGGATCTAC
    ATGAGTGACA ACTACACAGA TGTGAATGGG GATAAGGTGC TTCCTAAGGA TGACATTGAG
    TGCCCACTGG GCTGGAAGTG GGAAGATGAA GAGTGGTCCA CGGACCTCAA TCGGGCCGTC
    GATGAGCAAG GCTGGGAGTA CAGTATCACC ATCCCCCCGG ATCGGAAGCC AAAGCACTGG
    GTTCCTGCTG AGAAGATGTA TTACACGCAC CGTCGGCGGC GCTGGGTCCG CCTGCGCAGA
    AGGGACCTCA GCCAGATGGA GGCATTGAAG AGGCACAGAC AGGCAGAGGC AGAGGGTGAG
    GGCTGGGAGT ACGCCTCGCT CTTGGGCTGG AAGTTCCACC TGGAGTACCG CAAGACTGAT
    GCTTTCCGCC GCCGCCGCTG GCGCCGCCGC ATGGAACCGC TGGAGAAGAC GGGGCCTGCA
    GCCGTGTTTG CCCTTGAGGG GGCTCTGGGT GGTGTGGTAG ATGACAGGAG TGAAGATTCC
    GTGTCCGTGT CCACCTTGAG CTTTGGAGTG AACAGACCCA CGATTTCCTG CATCTTTGAT
    TATGGGAACC GCTACCACCT ACGCTGCTAC ATGTACCAGG CTCGGGACCT GCCCTCGATG
    GACAAGGACT CTTTTTCTGA TCCCTACGCC ATCGTCTCCT TCCTGCACCA GAGCCAGAAG
    ACAGTGGTGG CGAAGAACAC TCTGAACCCC ACCTGGGACC AGACGCTTAT CTTCTATGAG
    ATTGAGATCT TTGGTGAGCC AGCCAGTATC GCTGAGCAGC CGCCCAGCAT TGTGGTGGAG
    CTGTATGACC ACGACACCTA CGGTGCAGAT GAGTTTATGG GACGCTGCAT TTGTCAGCCC
    AGCCTGGAGC GGACACCCCG GCTGGCCTGG TTCCCACTGA TTAGGGGCAG CCAGTCGGCG
    GGGGAACTGC TGGCCTCTTT TGAGCTCATC CAGAGAGAAA AGCCGGCCAT TCACCATATT
    CCTGGTTTTG AGGTACAGGA TATGTCAGGA ATCCTGGAAG AGCTGTGTGC ACCTGCCTTC
    TTCCTCCAGG GCCTCCTCCA AACGTCAGAG GACATAGACC TGCCCTACCC GCCACCTCAG
    AGGGAGGCCA ACATCTTCAT GGTCCCCCAG AACATCAAGC CTGTTCTCCA GCGTACTGCC
    ATAGAGATCC TGGCGTGGGG CCTGCGGAAC ATGAAGAGCT ACCAGCTAGC CAGCATCTCC
    TCCCCTAGCC TTGTGGTAGA GTGCGGGGGC CAGACAGTGC AGTCCTGTGT CATCAAGAAC
    CTCCGGAAGA ACCCCAACTT CGACATCTGT ACCCTTTTCA TGGAAGTGAT GCTGCCCAAG
    GAGGAGGTCT ACTGTCCCTC CATCGTGATC AAGGTCATTG ATAACCGCCA GTTTGGCCGC
    CGGCCTGTGG TGGGCCAGTG TACCATCCGT TCCCTGGAGA GCTTCATGTG TGACCTGTAC
    TCCGAGGAGA ATCCGTCCCC ACAGGGCGGC CCAGATGATG TGAGTTTACT CAGTCCTGGG
    GAAGACGTGC TCATTGACAT CGATGACAAG GAGCCCCTCA TCCCCATGCA GCTTGCAGAT
    GGTCTGTCGG GCTCTGCCCC CACTAACATG GCATCTTCTC CATCCAGTCC TCATAAGGCC
    CTCCTGGAGG AAGAGTTCAT CGACTGGTGG AGCAAATTCT TTGCCTCCAC AGGGGAGAGG
    GAAAAGTGCG GCTCATACTT GGAGAAGGGC TTTGACACTC TAAAGGTCTA TGACACACAA
    CTGGAGAACG TGGAGGCCTT TGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTCTAC
    CGGGGCAAGA CTCAGGAGGA GACAGAGGAC CCATCTGTTA TTGGGGAGTT TAAGGGCCTC
    TTCAAAATCT ATCCCCTCCC AGAAGACCCG GCTGTTCCCA TGCCCCCGAG ACAATTCCAC
    CAGCTGGCTG CCCAAGGGCC TCAGGAGTGC CTGGTCCGCA TCTATATCAT CCGAGCATTT
    GGCCTTCAGC CCAAGGACCC CAATGGGAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG
    AAGAAATCAG TGAGTGACCA GGATAACTAC ATTCCCTGTA CTCTGGAGCC TGTGTTTGGA
    AAGATGTTCG AGCTGACCTG CACTCTGCCT CTGGAGAAGG ACCTGAAGAT CACACTCTAT
    GACTATGACC TCCTCTCCAA AGATGAAAAG ATTGGGGAGA CAGTCATTGA CCTGGAGGAT
    AGGCTGCTGT CCAAGTTTGG GGCTCGCTGC GGACTCCCAC AGACGTACTG TGTCTCTGGA
    CCCAACCAGT GGAGGGACCA GCTCCGCCCC TCCCAGCTTC TCCACCTCTT CTGCCAGCAG
    CGCAGACTCA AGGCTCCTGT GTACCTCACG GACCACGTGG TGTTCCAGGA TAAAGAATAC
    AGCATTGAAG AAATAGAGGC TGGCCGAGTC CCGAACCCAC ACCTGGGCCC AGTGGAAGAG
    CGTCTGGCCT TGCATGTCCT CCAGCAGCAG GGCCTGGTCC CTGAGCATGT GGAGTCCCGG
    CCTCTCTACA GCCCCCTGCA GCCAGACATT GAACAGGGGA AGCTGCAGAT GTGGATCGAC
    CTATTTCCCA AGGCCCTGGG GCGTCCCGGA CCTCCCTTCA ACATCACCCC GCGGAGAGCC
    AGAAGGTTTT TCCTGCGTTG TATCATCTGG AACACAAAGG ACGTGATCCT GGACGACCTC
    AGCATCACAG GGGAGAAGAT GAGTGATATT TATGTGAAGG GTTGGATGGT TGGCTTTGAA
    GAGCACAAGC AAAAGACAGA TGTGCATTAC CGGTCCCTGG GAGGTGAAGG CAACTTTAAC
    TGGAGGTTCA TTTTCCCCTT TGACTACCTG CCAGCCGAGC AAGTCTGCAC CGTCTCTAAG
    AAGGATGCTT TCTGGAGGCT GGACAAGACT GAGAGCAAAA TCCCAGCACG AGTGGTATTT
    CAGATCTGGG ACAATGACAA GTTCTCCTTT GACGACTTTC TGGGCTCCTT GCAGTTGGAT
    CTCAACCACA TGCCCAAGCC TGCCAAGACA GCTGAGAAGT GCTCCTTGGA CCAGCTGGAT
    GACACTTTCC ACCCGGAGTG GTTTGTGTCC CTTTTTGAAC AGAAAACAGT TAAGGGCTGG
    TGGCCCTGCG TAGCAGAAGA GGGCGAGAAG AAGATCCTGG CAGGCAAGCT GGAAATGACC
    TTGGAGATCG TAGCAGAGAG TGAGCATGAG GAGCGGCCTG CTGGCCAGGG CCGGGATGAA
    CCCAACATGA ACCCCAAGCT CGAGGACCCA AGGCGCCCTG ACACCTCCTT CCTCTGGTTC
    ACCTCTCCAT ACAAGACTAT GAAGTTCATC CTGTGGCGGC GGTTCCGATG TGCCATTATC
    CTCTTCATCA TTCTCTTCAT CCTGCTACTA TTCTTAGGCA TCTTCATCTA TGCTTTCCCG
    AACTACGCTG CCATGAAGCT GGTGAAGCCC TTCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022260555.1
    CDS23..6481
    Translation

    Target ORF information:

    RefSeq Version XM_022404847.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris dysferlin (DYSF), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022404847.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG
    CTGGCCCCCA CGGGAGGAGA GGAGGACACT GAGGACCAGG GGCTCACTGG AGACGAGGGA
    GAGCCATTAC TGGATCAGAG TGGCGTCCCA GGCCCTGGGG CTCCCACCAC CCCGAGGAAG
    CCACCTTCCC ATCTGCCCCC CTACCACCCT GGGAGCAAGA GGAAAAGAAG TGCTCCCGCA
    GCCAGAAAGC TGCTGTCCGA CAAACCGCAG GACTTCCAGA TCAGAGTGCA AGTGATCGAG
    GGCCGCCAGC TGCCAGGGGT GAATATCAAG CCTGTGATCA AGGTCACTGC TGCTGGGCAG
    ACCAAGAGGA CACGGATCCA CAAGGGAAAC AGCCCACTAT TTAACGAGAC TCTTTTCTTC
    AATGTGTTTG ACTCTCCTTC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTCGTAGAC
    TCCCGTTCTC TCAGGACAGA TGCTCTCATT GGGGAATTCC GGATGGATGT GGGTACCATC
    TACAGAGAGC CCCGACATGC TTATCTGAGG AAGTGGCTGC TACTCTCCAA CCCTGATGAC
    TTCTCTGTTG GGGCCAGAGG TTACCTGAAA GCAAGCCTTT ATGTGCTGGG GCCCGGAGAT
    GAAGCGCCTC TGGAGAGAAA GGACCCCTCT GAAGACAAGG AGGACATTGA AAACAATCTG
    CTCAGGCCAA CTGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGAT CTTCAGAGCG
    GAGGACTTAC CACAAATGGA TGATGCCGTG ATGGACAATG TGAAGCAGAT CTTTGGCTTT
    GACAGCAACA AGAAGAATTT GGTAGATCCC TTCATGGAAG TCAGCTTTGC GGGGAAAATG
    CTCTGCAGCA AGATCCTGGA GAAGACAGCC AACCCTCAGT GGAACCAGAG CATCACTCTG
    CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAAGATTC GCGTCATGGA CTGGGACCGC
    CTTACCCACA ATGACATCGT GGCCACCACC TACCTGAGTA TGTCGAAAAT CTCTGCCCCT
    GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GTGGTCAAGC CTTCTCCAGC CACGGACCTG
    GATGACCATC TGGGCTTCCT TCCCACCTTC GGGCCCTGCT TCGTCAATCT CTATGGCAGC
    CCCAGGGAAT TCACTGGCTT CCCGGACCCC TACGCGGAAC TCAACATGGG CAAGGGGGAA
    GGCGTGGCCT ACCGAGGTCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGC
    GGCGAGCAAA GGGTGGAGGA CCTCCCTGCT GATGACATCC TTCGGGTGGA GAAATACCTC
    CGGAGGCGCA AGTACTCTCT CTTTGCCGCC TTCTACTCGG CCACCATGCT CCAGGATGTC
    GACGATGCCA TCCAGTTTGA GGTCAGCATT GGGAACTATG GCAACAAGTT CGACACGACC
    TGTCTGCCAC TGGCCTCCAC CACCCAGTAC AGCCGGGCAG TCTTTGATGG GTGCCACTAC
    TACTACTTGC CCTGGGGCAA TGTGAAGCCT GTGGTGGTGC TGTCATCCTA CTGGGAAGAC
    ATCAGCCACA GAGTCGAGGC TCAGAACCAG CTGCTCAGGA TCGCTGACCA TCTGGAAGCT
    GGCCTGGAGC AAGTCCACTT GGCTTTAAAG GCGCAGTTCA ACACAGAGGA TGTGGACTCC
    CTGTTGGCCC AGCTGATGGA TGAGCTCATC TCAGACTGCA GCCAGCCCCT GGGCAATGTC
    CAAGAGATAC CCTCTGCCAC CCACCTGGAC CGGCACCTGT ACAGGCTGCG CACCCGTCAC
    CTAGGCCAGA TTACTGAGGC GGCCCTGGCT CTGAAACTCG GGCATGGCAA GCTCCCTGCC
    GCCCTGGAGC AGGCTGAAGA CTGGCTCCTG CGTCTCCGTG CCCTGGCTGA TGAGCCCCAG
    AACAGCCTGC CCGACATCAT CATCTGGATG CTGCAGGGAA ACAAGCGAGT GGCGTACCAG
    CGGGTGCCTG CGCACGAGGT CCTCTTCTCC CGGCGGGGCG CCAGCTACTG TGGCAAGAAC
    TGTGGGAAGC TGCAGACGAT ATTTCTGAAA TATCCCATGG AGGGGGTGCC CGGAGCCCGG
    ATGCCAGTAC AAATACGGGT GAAACTCTGG TTTGGGCTTT CTGTGGATGA GAAGGAGTTC
    AACCAGTTTG CTGAGGGAAA GCTGTCCGTC TTTGCTGAAA CTTATGAGAA CCAGACCAAA
    CTGGCCCTGG TTGGGAACTG GGGCACAACA GGCCTCACCT ACCCCAAGTT TTCTGATGTC
    ATGGGCAAGA TCAAGCTACC CAAGGATAGC TTCCGCCCCT CAGCTGGCTG GACCTGGGCC
    GGAGACTGGT TCGTGTGTCC AGAGAAGACC CTACTCCATG ACATGGACGC GGGCCACCTG
    AGTTTTGTGG AGGAGGTGTT TGAGAACCAG ACCCGGCTCC CTGGAGGCCA GTGGATCTAC
    ATGAGTGACA ACTACACAGA TGTGAATGGG GATAAGGTGC TTCCTAAGGA TGACATTGAG
    TGCCCACTGG GCTGGAAGTG GGAAGATGAA GAGTGGTCCA CGGACCTCAA TCGGGCCGTC
    GATGAGCAAG GCTGGGAGTA CAGTATCACC ATCCCCCCGG ATCGGAAGCC AAAGCACTGG
    GTTCCTGCTG AGAAGATGTA TTACACGCAC CGTCGGCGGC GCTGGGTCCG CCTGCGCAGA
    AGGGACCTCA GCCAGATGGA GGCATTGAAG AGGCACAGAC AGGCAGAGGC AGAGGGTGAG
    GGCTGGGAGT ACGCCTCGCT CTTGGGCTGG AAGTTCCACC TGGAGTACCG CAAGACTGAT
    GCTTTCCGCC GCCGCCGCTG GCGCCGCCGC ATGGAACCGC TGGAGAAGAC GGGGCCTGCA
    GCCGTGTTTG CCCTTGAGGG GGCTCTGGGT GGTGTGGTAG ATGACAGGAG TGAAGATTCC
    GTGTCCGTGT CCACCTTGAG CTTTGGAGTG AACAGACCCA CGATTTCCTG CATCTTTGAT
    TATGGGAACC GCTACCACCT ACGCTGCTAC ATGTACCAGG CTCGGGACCT GCCCTCGATG
    GACAAGGACT CTTTTTCTGA TCCCTACGCC ATCGTCTCCT TCCTGCACCA GAGCCAGAAG
    ACAGTGGTGG CGAAGAACAC TCTGAACCCC ACCTGGGACC AGACGCTTAT CTTCTATGAG
    ATTGAGATCT TTGGTGAGCC AGCCAGTATC GCTGAGCAGC CGCCCAGCAT TGTGGTGGAG
    CTGTATGACC ACGACACCTA CGGTGCAGAT GAGTTTATGG GACGCTGCAT TTGTCAGCCC
    AGCCTGGAGC GGACACCCCG GCTGGCCTGG TTCCCACTGA TTAGGGGCAG CCAGTCGGCG
    GGGGAACTGC TGGCCTCTTT TGAGCTCATC CAGAGAGAAA AGCCGGCCAT TCACCATATT
    CCTGGTTTTG AGGTACAGGA TATGTCAGGA ATCCTGGAAG AGCTGTGTGC ACCTGCCTTC
    TTCCTCCAGG GCCTCCTCCA AACGTCAGAG GACATAGACC TGCCCTACCC GCCACCTCAG
    AGGGAGGCCA ACATCTTCAT GGTCCCCCAG AACATCAAGC CTGTTCTCCA GCGTACTGCC
    ATAGAGATCC TGGCGTGGGG CCTGCGGAAC ATGAAGAGCT ACCAGCTAGC CAGCATCTCC
    TCCCCTAGCC TTGTGGTAGA GTGCGGGGGC CAGACAGTGC AGTCCTGTGT CATCAAGAAC
    CTCCGGAAGA ACCCCAACTT CGACATCTGT ACCCTTTTCA TGGAAGTGAT GCTGCCCAAG
    GAGGAGGTCT ACTGTCCCTC CATCGTGATC AAGGTCATTG ATAACCGCCA GTTTGGCCGC
    CGGCCTGTGG TGGGCCAGTG TACCATCCGT TCCCTGGAGA GCTTCATGTG TGACCTGTAC
    TCCGAGGAGA ATCCGTCCCC ACAGGGCGGC CCAGATGATG TGAGTTTACT CAGTCCTGGG
    GAAGACGTGC TCATTGACAT CGATGACAAG GAGCCCCTCA TCCCCATGCA GCTTGCAGAT
    GGTCTGTCGG GCTCTGCCCC CACTAACATG GCATCTTCTC CATCCAGTCC TCATAAGGCC
    CTCCTGGAGG AAGAGTTCAT CGACTGGTGG AGCAAATTCT TTGCCTCCAC AGGGGAGAGG
    GAAAAGTGCG GCTCATACTT GGAGAAGGGC TTTGACACTC TAAAGGTCTA TGACACACAA
    CTGGAGAACG TGGAGGCCTT TGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTCTAC
    CGGGGCAAGA CTCAGGAGGA GACAGAGGAC CCATCTGTTA TTGGGGAGTT TAAGGGCCTC
    TTCAAAATCT ATCCCCTCCC AGAAGACCCG GCTGTTCCCA TGCCCCCGAG ACAATTCCAC
    CAGCTGGCTG CCCAAGGGCC TCAGGAGTGC CTGGTCCGCA TCTATATCAT CCGAGCATTT
    GGCCTTCAGC CCAAGGACCC CAATGGGAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG
    AAGAAATCAG TGAGTGACCA GGATAACTAC ATTCCCTGTA CTCTGGAGCC TGTGTTTGGA
    AAGATGTTCG AGCTGACCTG CACTCTGCCT CTGGAGAAGG ACCTGAAGAT CACACTCTAT
    GACTATGACC TCCTCTCCAA AGATGAAAAG ATTGGGGAGA CAGTCATTGA CCTGGAGGAT
    AGGCTGCTGT CCAAGTTTGG GGCTCGCTGC GGACTCCCAC AGACGTACTG TGTCTCTGGA
    CCCAACCAGT GGAGGGACCA GCTCCGCCCC TCCCAGCTTC TCCACCTCTT CTGCCAGCAG
    CGCAGACTCA AGGCTCCTGT GTACCTCACG GACCACGTGG TGTTCCAGGA TAAAGAATAC
    AGCATTGAAG AAATAGAGGC TGGCCGAGTC CCGAACCCAC ACCTGGGCCC AGTGGAAGAG
    CGTCTGGCCT TGCATGTCCT CCAGCAGCAG GGCCTGGTCC CTGAGCATGT GGAGTCCCGG
    CCTCTCTACA GCCCCCTGCA GCCAGACATT GAACAGGGGA AGCTGCAGAT GTGGATCGAC
    CTATTTCCCA AGGCCCTGGG GCGTCCCGGA CCTCCCTTCA ACATCACCCC GCGGAGAGCC
    AGAAGGTTTT TCCTGCGTTG TATCATCTGG AACACAAAGG ACGTGATCCT GGACGACCTC
    AGCATCACAG GGGAGAAGAT GAGTGATATT TATGTGAAGG GTTGGATGGT TGGCTTTGAA
    GAGCACAAGC AAAAGACAGA TGTGCATTAC CGGTCCCTGG GAGGTGAAGG CAACTTTAAC
    TGGAGGTTCA TTTTCCCCTT TGACTACCTG CCAGCCGAGC AAGTCTGCAC CGTCTCTAAG
    AAGGATGCTT TCTGGAGGCT GGACAAGACT GAGAGCAAAA TCCCAGCACG AGTGGTATTT
    CAGATCTGGG ACAATGACAA GTTCTCCTTT GACGACTTTC TGGGCTCCTT GCAGTTGGAT
    CTCAACCACA TGCCCAAGCC TGCCAAGACA GCTGAGAAGT GCTCCTTGGA CCAGCTGGAT
    GACACTTTCC ACCCGGAGTG GTTTGTGTCC CTTTTTGAAC AGAAAACAGT TAAGGGCTGG
    TGGCCCTGCG TAGCAGAAGA GGGCGAGAAG AAGATCCTGG CAGGCAAGCT GGAAATGACC
    TTGGAGATCG TAGCAGAGAG TGAGCATGAG GAGCGGCCTG CTGGCCAGGG CCGGGATGAA
    CCCAACATGA ACCCCAAGCT CGAGGACCCA AGGCGCCCTG ACACCTCCTT CCTCTGGTTC
    ACCTCTCCAT ACAAGACTAT GAAGTTCATC CTGTGGCGGC GGTTCCGATG TGCCATTATC
    CTCTTCATCA TTCTCTTCAT CCTGCTACTA TTCTTAGGCA TCTTCATCTA TGCTTTCCCG
    AACTACGCTG CCATGAAGCT GGTGAAGCCC TTCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe294457
    Clone ID Related Accession (Same CDS sequence) XM_022404848.1
    Accession Version XM_022404848.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6453bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product dysferlin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG
    CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC
    GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG
    AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT
    CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG
    ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT
    GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT
    TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC
    GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT
    ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT
    GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC
    GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC
    AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC
    AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC
    ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG
    GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT
    GCCCCTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTGG TCAAGCCTTC TCCAGCCACG
    GACCTGGATG ACCATCTGGG CTTCCTTCCC ACCTTCGGGC CCTGCTTCGT CAATCTCTAT
    GGCAGCCCCA GGGAATTCAC TGGCTTCCCG GACCCCTACG CGGAACTCAA CATGGGCAAG
    GGGGAAGGCG TGGCCTACCG AGGTCGGCTT CTGCTCTCCC TGGAGACCAA GCTGGTGGAG
    CACAGCGGCG AGCAAAGGGT GGAGGACCTC CCTGCTGATG ACATCCTTCG GGTGGAGAAA
    TACCTCCGGA GGCGCAAGTA CTCTCTCTTT GCCGCCTTCT ACTCGGCCAC CATGCTCCAG
    GATGTCGACG ATGCCATCCA GTTTGAGGTC AGCATTGGGA ACTATGGCAA CAAGTTCGAC
    ACGACCTGTC TGCCACTGGC CTCCACCACC CAGTACAGCC GGGCAGTCTT TGATGGGTGC
    CACTACTACT ACTTGCCCTG GGGCAATGTG AAGCCTGTGG TGGTGCTGTC ATCCTACTGG
    GAAGACATCA GCCACAGAGT CGAGGCTCAG AACCAGCTGC TCAGGATCGC TGACCATCTG
    GAAGCTGGCC TGGAGCAAGT CCACTTGGCT TTAAAGGCGC AGTTCAACAC AGAGGATGTG
    GACTCCCTGT TGGCCCAGCT GATGGATGAG CTCATCTCAG ACTGCAGCCA GCCCCTGGGC
    AATGTCCAAG AGATACCCTC TGCCACCCAC CTGGACCGGC ACCTGTACAG GCTGCGCACC
    CGTCACCTAG GCCAGATTAC TGAGGCGGCC CTGGCTCTGA AACTCGGGCA TGGCAAGCTC
    CCTGCCGCCC TGGAGCAGGC TGAAGACTGG CTCCTGCGTC TCCGTGCCCT GGCTGATGAG
    CCCCAGAACA GCCTGCCCGA CATCATCATC TGGATGCTGC AGGGAAACAA GCGAGTGGCG
    TACCAGCGGG TGCCTGCGCA CGAGGTCCTC TTCTCCCGGC GGGGCGCCAG CTACTGTGGC
    AAGAACTGTG GGAAGCTGCA GACGATATTT CTGAAATATC CCATGGAGGG GGTGCCCGGA
    GCCCGGATGC CAGTACAAAT ACGGGTGAAA CTCTGGTTTG GGCTTTCTGT GGATGAGAAG
    GAGTTCAACC AGTTTGCTGA GGGAAAGCTG TCCGTCTTTG CTGAAACTTA TGAGAACCAG
    ACCAAACTGG CCCTGGTTGG GAACTGGGGC ACAACAGGCC TCACCTACCC CAAGTTTTCT
    GATGTCATGG GCAAGATCAA GCTACCCAAG GATAGCTTCC GCCCCTCAGC TGGCTGGACC
    TGGGCCGGAG ACTGGTTCGT GTGTCCAGAG AAGACCCTAC TCCATGACAT GGACGCGGGC
    CACCTGAGTT TTGTGGAGGA GGTGTTTGAG AACCAGACCC GGCTCCCTGG AGGCCAGTGG
    ATCTACATGA GTGACAACTA CACAGATGTG AATGGGGATA AGGTGCTTCC TAAGGATGAC
    ATTGAGTGCC CACTGGGCTG GAAGTGGGAA GATGAAGAGT GGTCCACGGA CCTCAATCGG
    GCCGTCGATG AGCAAGGCTG GGAGTACAGT ATCACCATCC CCCCGGATCG GAAGCCAAAG
    CACTGGGTTC CTGCTGAGAA GATGTATTAC ACGCACCGTC GGCGGCGCTG GGTCCGCCTG
    CGCAGAAGGG ACCTCAGCCA GATGGAGGCA TTGAAGAGGC ACAGACAGGC AGAGGCAGAG
    GGTGAGGGCT GGGAGTACGC CTCGCTCTTG GGCTGGAAGT TCCACCTGGA GTACCGCAAG
    ACTGATGCTT TCCGCCGCCG CCGCTGGCGC CGCCGCATGG AACCGCTGGA GAAGACGGGG
    CCTGCAGCCG TGTTTGCCCT TGAGGGGGCT CTGGGTGGTG TGGTAGATGA CAGGAGTGAA
    GATTCCGTGT CCGTGTCCAC CTTGAGCTTT GGAGTGAACA GACCCACGAT TTCCTGCATC
    TTTGATTATG GGAACCGCTA CCACCTACGC TGCTACATGT ACCAGGCTCG GGACCTGCCC
    TCGATGGACA AGGACTCTTT TTCTGATCCC TACGCCATCG TCTCCTTCCT GCACCAGAGC
    CAGAAGACAG TGGTGGCGAA GAACACTCTG AACCCCACCT GGGACCAGAC GCTTATCTTC
    TATGAGATTG AGATCTTTGG TGAGCCAGCC AGTATCGCTG AGCAGCCGCC CAGCATTGTG
    GTGGAGCTGT ATGACCACGA CACCTACGGT GCAGATGAGT TTATGGGACG CTGCATTTGT
    CAGCCCAGCC TGGAGCGGAC ACCCCGGCTG GCCTGGTTCC CACTGATTAG GGGCAGCCAG
    TCGGCGGGGG AACTGCTGGC CTCTTTTGAG CTCATCCAGA GAGAAAAGCC GGCCATTCAC
    CATATTCCTG GTTTTGAGGT ACAGGATATG TCAGGAATCC TGGAAGAGCT GTGTGCACCT
    GCCTTCTTCC TCCAGGGCCT CCTCCAAACG TCAGAGGACA TAGACCTGCC CTACCCGCCA
    CCTCAGAGGG AGGCCAACAT CTTCATGGTC CCCCAGAACA TCAAGCCTGT TCTCCAGCGT
    ACTGCCATAG AGATCCTGGC GTGGGGCCTG CGGAACATGA AGAGCTACCA GCTAGCCAGC
    ATCTCCTCCC CTAGCCTTGT GGTAGAGTGC GGGGGCCAGA CAGTGCAGTC CTGTGTCATC
    AAGAACCTCC GGAAGAACCC CAACTTCGAC ATCTGTACCC TTTTCATGGA AGTGATGCTG
    CCCAAGGAGG AGGTCTACTG TCCCTCCATC GTGATCAAGG TCATTGATAA CCGCCAGTTT
    GGCCGCCGGC CTGTGGTGGG CCAGTGTACC ATCCGTTCCC TGGAGAGCTT CATGTGTGAC
    CTGTACTCCG AGGAGAATCC GTCCCCACAG GGCGGCCCAG ATGATGTGAG TTTACTCAGT
    CCTGGGGAAG ACGTGCTCAT TGACATCGAT GACAAGGAGC CCCTCATCCC CATGCAGCTT
    GCAGATGGTC TGTCGGGCTC TGCCCCCACT AACATGGCAT CTTCTCCATC CAGTCCTCAT
    GAGGAAGAGT TCATCGACTG GTGGAGCAAA TTCTTTGCCT CCACAGGGGA GAGGGAAAAG
    TGCGGCTCAT ACTTGGAGAA GGGCTTTGAC ACTCTAAAGG TCTATGACAC ACAACTGGAG
    AACGTGGAGG CCTTTGAGGG CCTGTCTGAC TTTTGTAACA CCTTCAAGCT CTACCGGGGC
    AAGACTCAGG AGGAGACAGA GGACCCATCT GTTATTGGGG AGTTTAAGGG CCTCTTCAAA
    ATCTATCCCC TCCCAGAAGA CCCGGCTGTT CCCATGCCCC CGAGACAATT CCACCAGCTG
    GCTGCCCAAG GGCCTCAGGA GTGCCTGGTC CGCATCTATA TCATCCGAGC ATTTGGCCTT
    CAGCCCAAGG ACCCCAATGG GAAGTGTGAT CCTTACATCA AGATCTCCAT AGGGAAGAAA
    TCAGTGAGTG ACCAGGATAA CTACATTCCC TGTACTCTGG AGCCTGTGTT TGGAAAGATG
    TTCGAGCTGA CCTGCACTCT GCCTCTGGAG AAGGACCTGA AGATCACACT CTATGACTAT
    GACCTCCTCT CCAAAGATGA AAAGATTGGG GAGACAGTCA TTGACCTGGA GGATAGGCTG
    CTGTCCAAGT TTGGGGCTCG CTGCGGACTC CCACAGACGT ACTGTGTCTC TGGACCCAAC
    CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG CTTCTCCACC TCTTCTGCCA GCAGCGCAGA
    CTCAAGGCTC CTGTGTACCT CACGGACCAC GTGGTGTTCC AGGATAAAGA ATACAGCATT
    GAAGAAATAG AGGCTGGCCG AGTCCCGAAC CCACACCTGG GCCCAGTGGA AGAGCGTCTG
    GCCTTGCATG TCCTCCAGCA GCAGGGCCTG GTCCCTGAGC ATGTGGAGTC CCGGCCTCTC
    TACAGCCCCC TGCAGCCAGA CATTGAACAG GGGAAGCTGC AGATGTGGAT CGACCTATTT
    CCCAAGGCCC TGGGGCGTCC CGGACCTCCC TTCAACATCA CCCCGCGGAG AGCCAGAAGG
    TTTTTCCTGC GTTGTATCAT CTGGAACACA AAGGACGTGA TCCTGGACGA CCTCAGCATC
    ACAGGGGAGA AGATGAGTGA TATTTATGTG AAGGGTTGGA TGGTTGGCTT TGAAGAGCAC
    AAGCAAAAGA CAGATGTGCA TTACCGGTCC CTGGGAGGTG AAGGCAACTT TAACTGGAGG
    TTCATTTTCC CCTTTGACTA CCTGCCAGCC GAGCAAGTCT GCACCGTCTC TAAGAAGGAT
    GCTTTCTGGA GGCTGGACAA GACTGAGAGC AAAATCCCAG CACGAGTGGT ATTTCAGATC
    TGGGACAATG ACAAGTTCTC CTTTGACGAC TTTCTGGGCT CCTTGCAGTT GGATCTCAAC
    CACATGCCCA AGCCTGCCAA GACAGCTGAG AAGTGCTCCT TGGACCAGCT GGATGACACT
    TTCCACCCGG AGTGGTTTGT GTCCCTTTTT GAACAGAAAA CAGTTAAGGG CTGGTGGCCC
    TGCGTAGCAG AAGAGGGCGA GAAGAAGATC CTGGCAGGCA AGCTGGAAAT GACCTTGGAG
    ATCGTAGCAG AGAGTGAGCA TGAGGAGCGG CCTGCTGGCC AGGGCCGGGA TGAACCCAAC
    ATGAACCCCA AGCTCGAGGA CCCAAGGCGC CCTGACACCT CCTTCCTCTG GTTCACCTCT
    CCATACAAGA CTATGAAGTT CATCCTGTGG CGGCGGTTCC GATGTGCCAT TATCCTCTTC
    ATCATTCTCT TCATCCTGCT ACTATTCTTA GGCATCTTCA TCTATGCTTT CCCGAACTAC
    GCTGCCATGA AGCTGGTGAA GCCCTTCAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022260556.1
    CDS23..6475
    Translation

    Target ORF information:

    RefSeq Version XM_022404848.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris dysferlin (DYSF), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022404848.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG
    CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC
    GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG
    AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT
    CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG
    ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT
    GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT
    TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC
    GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT
    ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT
    GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC
    GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC
    AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC
    AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC
    ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG
    GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT
    GCCCCTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTGG TCAAGCCTTC TCCAGCCACG
    GACCTGGATG ACCATCTGGG CTTCCTTCCC ACCTTCGGGC CCTGCTTCGT CAATCTCTAT
    GGCAGCCCCA GGGAATTCAC TGGCTTCCCG GACCCCTACG CGGAACTCAA CATGGGCAAG
    GGGGAAGGCG TGGCCTACCG AGGTCGGCTT CTGCTCTCCC TGGAGACCAA GCTGGTGGAG
    CACAGCGGCG AGCAAAGGGT GGAGGACCTC CCTGCTGATG ACATCCTTCG GGTGGAGAAA
    TACCTCCGGA GGCGCAAGTA CTCTCTCTTT GCCGCCTTCT ACTCGGCCAC CATGCTCCAG
    GATGTCGACG ATGCCATCCA GTTTGAGGTC AGCATTGGGA ACTATGGCAA CAAGTTCGAC
    ACGACCTGTC TGCCACTGGC CTCCACCACC CAGTACAGCC GGGCAGTCTT TGATGGGTGC
    CACTACTACT ACTTGCCCTG GGGCAATGTG AAGCCTGTGG TGGTGCTGTC ATCCTACTGG
    GAAGACATCA GCCACAGAGT CGAGGCTCAG AACCAGCTGC TCAGGATCGC TGACCATCTG
    GAAGCTGGCC TGGAGCAAGT CCACTTGGCT TTAAAGGCGC AGTTCAACAC AGAGGATGTG
    GACTCCCTGT TGGCCCAGCT GATGGATGAG CTCATCTCAG ACTGCAGCCA GCCCCTGGGC
    AATGTCCAAG AGATACCCTC TGCCACCCAC CTGGACCGGC ACCTGTACAG GCTGCGCACC
    CGTCACCTAG GCCAGATTAC TGAGGCGGCC CTGGCTCTGA AACTCGGGCA TGGCAAGCTC
    CCTGCCGCCC TGGAGCAGGC TGAAGACTGG CTCCTGCGTC TCCGTGCCCT GGCTGATGAG
    CCCCAGAACA GCCTGCCCGA CATCATCATC TGGATGCTGC AGGGAAACAA GCGAGTGGCG
    TACCAGCGGG TGCCTGCGCA CGAGGTCCTC TTCTCCCGGC GGGGCGCCAG CTACTGTGGC
    AAGAACTGTG GGAAGCTGCA GACGATATTT CTGAAATATC CCATGGAGGG GGTGCCCGGA
    GCCCGGATGC CAGTACAAAT ACGGGTGAAA CTCTGGTTTG GGCTTTCTGT GGATGAGAAG
    GAGTTCAACC AGTTTGCTGA GGGAAAGCTG TCCGTCTTTG CTGAAACTTA TGAGAACCAG
    ACCAAACTGG CCCTGGTTGG GAACTGGGGC ACAACAGGCC TCACCTACCC CAAGTTTTCT
    GATGTCATGG GCAAGATCAA GCTACCCAAG GATAGCTTCC GCCCCTCAGC TGGCTGGACC
    TGGGCCGGAG ACTGGTTCGT GTGTCCAGAG AAGACCCTAC TCCATGACAT GGACGCGGGC
    CACCTGAGTT TTGTGGAGGA GGTGTTTGAG AACCAGACCC GGCTCCCTGG AGGCCAGTGG
    ATCTACATGA GTGACAACTA CACAGATGTG AATGGGGATA AGGTGCTTCC TAAGGATGAC
    ATTGAGTGCC CACTGGGCTG GAAGTGGGAA GATGAAGAGT GGTCCACGGA CCTCAATCGG
    GCCGTCGATG AGCAAGGCTG GGAGTACAGT ATCACCATCC CCCCGGATCG GAAGCCAAAG
    CACTGGGTTC CTGCTGAGAA GATGTATTAC ACGCACCGTC GGCGGCGCTG GGTCCGCCTG
    CGCAGAAGGG ACCTCAGCCA GATGGAGGCA TTGAAGAGGC ACAGACAGGC AGAGGCAGAG
    GGTGAGGGCT GGGAGTACGC CTCGCTCTTG GGCTGGAAGT TCCACCTGGA GTACCGCAAG
    ACTGATGCTT TCCGCCGCCG CCGCTGGCGC CGCCGCATGG AACCGCTGGA GAAGACGGGG
    CCTGCAGCCG TGTTTGCCCT TGAGGGGGCT CTGGGTGGTG TGGTAGATGA CAGGAGTGAA
    GATTCCGTGT CCGTGTCCAC CTTGAGCTTT GGAGTGAACA GACCCACGAT TTCCTGCATC
    TTTGATTATG GGAACCGCTA CCACCTACGC TGCTACATGT ACCAGGCTCG GGACCTGCCC
    TCGATGGACA AGGACTCTTT TTCTGATCCC TACGCCATCG TCTCCTTCCT GCACCAGAGC
    CAGAAGACAG TGGTGGCGAA GAACACTCTG AACCCCACCT GGGACCAGAC GCTTATCTTC
    TATGAGATTG AGATCTTTGG TGAGCCAGCC AGTATCGCTG AGCAGCCGCC CAGCATTGTG
    GTGGAGCTGT ATGACCACGA CACCTACGGT GCAGATGAGT TTATGGGACG CTGCATTTGT
    CAGCCCAGCC TGGAGCGGAC ACCCCGGCTG GCCTGGTTCC CACTGATTAG GGGCAGCCAG
    TCGGCGGGGG AACTGCTGGC CTCTTTTGAG CTCATCCAGA GAGAAAAGCC GGCCATTCAC
    CATATTCCTG GTTTTGAGGT ACAGGATATG TCAGGAATCC TGGAAGAGCT GTGTGCACCT
    GCCTTCTTCC TCCAGGGCCT CCTCCAAACG TCAGAGGACA TAGACCTGCC CTACCCGCCA
    CCTCAGAGGG AGGCCAACAT CTTCATGGTC CCCCAGAACA TCAAGCCTGT TCTCCAGCGT
    ACTGCCATAG AGATCCTGGC GTGGGGCCTG CGGAACATGA AGAGCTACCA GCTAGCCAGC
    ATCTCCTCCC CTAGCCTTGT GGTAGAGTGC GGGGGCCAGA CAGTGCAGTC CTGTGTCATC
    AAGAACCTCC GGAAGAACCC CAACTTCGAC ATCTGTACCC TTTTCATGGA AGTGATGCTG
    CCCAAGGAGG AGGTCTACTG TCCCTCCATC GTGATCAAGG TCATTGATAA CCGCCAGTTT
    GGCCGCCGGC CTGTGGTGGG CCAGTGTACC ATCCGTTCCC TGGAGAGCTT CATGTGTGAC
    CTGTACTCCG AGGAGAATCC GTCCCCACAG GGCGGCCCAG ATGATGTGAG TTTACTCAGT
    CCTGGGGAAG ACGTGCTCAT TGACATCGAT GACAAGGAGC CCCTCATCCC CATGCAGCTT
    GCAGATGGTC TGTCGGGCTC TGCCCCCACT AACATGGCAT CTTCTCCATC CAGTCCTCAT
    GAGGAAGAGT TCATCGACTG GTGGAGCAAA TTCTTTGCCT CCACAGGGGA GAGGGAAAAG
    TGCGGCTCAT ACTTGGAGAA GGGCTTTGAC ACTCTAAAGG TCTATGACAC ACAACTGGAG
    AACGTGGAGG CCTTTGAGGG CCTGTCTGAC TTTTGTAACA CCTTCAAGCT CTACCGGGGC
    AAGACTCAGG AGGAGACAGA GGACCCATCT GTTATTGGGG AGTTTAAGGG CCTCTTCAAA
    ATCTATCCCC TCCCAGAAGA CCCGGCTGTT CCCATGCCCC CGAGACAATT CCACCAGCTG
    GCTGCCCAAG GGCCTCAGGA GTGCCTGGTC CGCATCTATA TCATCCGAGC ATTTGGCCTT
    CAGCCCAAGG ACCCCAATGG GAAGTGTGAT CCTTACATCA AGATCTCCAT AGGGAAGAAA
    TCAGTGAGTG ACCAGGATAA CTACATTCCC TGTACTCTGG AGCCTGTGTT TGGAAAGATG
    TTCGAGCTGA CCTGCACTCT GCCTCTGGAG AAGGACCTGA AGATCACACT CTATGACTAT
    GACCTCCTCT CCAAAGATGA AAAGATTGGG GAGACAGTCA TTGACCTGGA GGATAGGCTG
    CTGTCCAAGT TTGGGGCTCG CTGCGGACTC CCACAGACGT ACTGTGTCTC TGGACCCAAC
    CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG CTTCTCCACC TCTTCTGCCA GCAGCGCAGA
    CTCAAGGCTC CTGTGTACCT CACGGACCAC GTGGTGTTCC AGGATAAAGA ATACAGCATT
    GAAGAAATAG AGGCTGGCCG AGTCCCGAAC CCACACCTGG GCCCAGTGGA AGAGCGTCTG
    GCCTTGCATG TCCTCCAGCA GCAGGGCCTG GTCCCTGAGC ATGTGGAGTC CCGGCCTCTC
    TACAGCCCCC TGCAGCCAGA CATTGAACAG GGGAAGCTGC AGATGTGGAT CGACCTATTT
    CCCAAGGCCC TGGGGCGTCC CGGACCTCCC TTCAACATCA CCCCGCGGAG AGCCAGAAGG
    TTTTTCCTGC GTTGTATCAT CTGGAACACA AAGGACGTGA TCCTGGACGA CCTCAGCATC
    ACAGGGGAGA AGATGAGTGA TATTTATGTG AAGGGTTGGA TGGTTGGCTT TGAAGAGCAC
    AAGCAAAAGA CAGATGTGCA TTACCGGTCC CTGGGAGGTG AAGGCAACTT TAACTGGAGG
    TTCATTTTCC CCTTTGACTA CCTGCCAGCC GAGCAAGTCT GCACCGTCTC TAAGAAGGAT
    GCTTTCTGGA GGCTGGACAA GACTGAGAGC AAAATCCCAG CACGAGTGGT ATTTCAGATC
    TGGGACAATG ACAAGTTCTC CTTTGACGAC TTTCTGGGCT CCTTGCAGTT GGATCTCAAC
    CACATGCCCA AGCCTGCCAA GACAGCTGAG AAGTGCTCCT TGGACCAGCT GGATGACACT
    TTCCACCCGG AGTGGTTTGT GTCCCTTTTT GAACAGAAAA CAGTTAAGGG CTGGTGGCCC
    TGCGTAGCAG AAGAGGGCGA GAAGAAGATC CTGGCAGGCA AGCTGGAAAT GACCTTGGAG
    ATCGTAGCAG AGAGTGAGCA TGAGGAGCGG CCTGCTGGCC AGGGCCGGGA TGAACCCAAC
    ATGAACCCCA AGCTCGAGGA CCCAAGGCGC CCTGACACCT CCTTCCTCTG GTTCACCTCT
    CCATACAAGA CTATGAAGTT CATCCTGTGG CGGCGGTTCC GATGTGCCAT TATCCTCTTC
    ATCATTCTCT TCATCCTGCT ACTATTCTTA GGCATCTTCA TCTATGCTTT CCCGAACTAC
    GCTGCCATGA AGCTGGTGAA GCCCTTCAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe294458
    Clone ID Related Accession (Same CDS sequence) XM_022404849.1
    Accession Version XM_022404849.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6423bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product dysferlin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG
    CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC
    GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG
    AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT
    CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG
    ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT
    GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT
    TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC
    GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT
    ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT
    GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC
    GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC
    AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC
    AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC
    ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG
    GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT
    GCCCCTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTGG TCAAGCCTTC TCCAGCCACG
    GACCTGGATG ACCATCTGGG CTTCCTTCCC ACCTTCGGGC CCTGCTTCGT CAATCTCTAT
    GGCAGCCCCA GGGAATTCAC TGGCTTCCCG GACCCCTACG CGGAACTCAA CATGGGCAAG
    GGGGAAGGCG TGGCCTACCG AGGTCGGCTT CTGCTCTCCC TGGAGACCAA GCTGGTGGAG
    CACAGCGGCG AGCAAAGGGT GGAGGACCTC CCTGCTGATG ACATCCTTCG GGTGGAGAAA
    TACCTCCGGA GGCGCAAGTA CTCTCTCTTT GCCGCCTTCT ACTCGGCCAC CATGCTCCAG
    GATGTCGACG ATGCCATCCA GTTTGAGGTC AGCATTGGGA ACTATGGCAA CAAGTTCGAC
    ACGACCTGTC TGCCACTGGC CTCCACCACC CAGTACAGCC GGGCAGTCTT TGATGGGTGC
    CACTACTACT ACTTGCCCTG GGGCAATGTG AAGCCTGTGG TGGTGCTGTC ATCCTACTGG
    GAAGACATCA GCCACAGAGT CGAGGCTCAG AACCAGCTGC TCAGGATCGC TGACCATCTG
    GAAGCTGGCC TGGAGCAAGT CCACTTGGCT TTAAAGGCGC AGTTCAACAC AGAGGATGTG
    GACTCCCTGT TGGCCCAGCT GATGGATGAG CTCATCTCAG ACTGCAGCCA GCCCCTGGGC
    AATGTCCAAG AGATACCCTC TGCCACCCAC CTGGACCGGC ACCTGTACAG GCTGCGCACC
    CGTCACCTAG GCCAGATTAC TGAGGCGGCC CTGGCTCTGA AACTCGGGCA TGGCAAGCTC
    CCTGCCGCCC TGGAGCAGGC TGAAGACTGG CTCCTGCGTC TCCGTGCCCT GGCTGATGAG
    CCCCAGAACA GCCTGCCCGA CATCATCATC TGGATGCTGC AGGGAAACAA GCGAGTGGCG
    TACCAGCGGG TGCCTGCGCA CGAGGTCCTC TTCTCCCGGC GGGGCGCCAG CTACTGTGGC
    AAGAACTGTG GGAAGCTGCA GACGATATTT CTGAAATATC CCATGGAGGG GGTGCCCGGA
    GCCCGGATGC CAGTACAAAT ACGGGTGAAA CTCTGGTTTG GGCTTTCTGT GGATGAGAAG
    GAGTTCAACC AGTTTGCTGA GGGAAAGCTG TCCGTCTTTG CTGAAACTTA TGAGAACCAG
    ACCAAACTGG CCCTGGTTGG GAACTGGGGC ACAACAGGCC TCACCTACCC CAAGTTTTCT
    GATGTCATGG GCAAGATCAA GCTACCCAAG GATAGCTTCC GCCCCTCAGC TGGCTGGACC
    TGGGCCGGAG ACTGGTTCGT GTGTCCAGAG AAGACCCTAC TCCATGACAT GGACGCGGGC
    CACCTGAGTT TTGTGGAGGA GGTGTTTGAG AACCAGACCC GGCTCCCTGG AGGCCAGTGG
    ATCTACATGA GTGACAACTA CACAGATGTG AATGGGGATA AGGTGCTTCC TAAGGATGAC
    ATTGAGTGCC CACTGGGCTG GAAGTGGGAA GATGAAGAGT GGTCCACGGA CCTCAATCGG
    GCCGTCGATG AGCAAGGCTG GGAGTACAGT ATCACCATCC CCCCGGATCG GAAGCCAAAG
    CACTGGGTTC CTGCTGAGAA GATGTATTAC ACGCACCGTC GGCGGCGCTG GGTCCGCCTG
    CGCAGAAGGG ACCTCAGCCA GATGGAGGCA TTGAAGAGGC ACAGACAGGC AGAGGCAGAG
    GGTGAGGGCT GGGAGTACGC CTCGCTCTTG GGCTGGAAGT TCCACCTGGA GTACCGCAAG
    ACTGATGCTT TCCGCCGCCG CCGCTGGCGC CGCCGCATGG AACCGCTGGA GAAGACGGGG
    CCTGCAGCCG TGTTTGCCCT TGAGGGGGCT CTGGGTGGTG TGGTAGATGA CAGGAGTGAA
    GATTCCGTGT CCGTGTCCAC CTTGAGCTTT GGAGTGAACA GACCCACGAT TTCCTGCATC
    TTTGATTATG GGAACCGCTA CCACCTACGC TGCTACATGT ACCAGGCTCG GGACCTGCCC
    TCGATGGACA AGGACTCTTT TTCTGATCCC TACGCCATCG TCTCCTTCCT GCACCAGAGC
    CAGAAGACAG TGGTGGCGAA GAACACTCTG AACCCCACCT GGGACCAGAC GCTTATCTTC
    TATGAGATTG AGATCTTTGG TGAGCCAGCC AGTATCGCTG AGCAGCCGCC CAGCATTGTG
    GTGGAGCTGT ATGACCACGA CACCTACGGT GCAGATGAGT TTATGGGACG CTGCATTTGT
    CAGCCCAGCC TGGAGCGGAC ACCCCGGCTG GCCTGGTTCC CACTGATTAG GGGCAGCCAG
    TCGGCGGGGG AACTGCTGGC CTCTTTTGAG CTCATCCAGA GAGAAAAGCC GGCCATTCAC
    CATATTCCTG GTTTTGAGGT ACAGGATATG TCAGGAATCC TGGAAGAGTC AGAGGACATA
    GACCTGCCCT ACCCGCCACC TCAGAGGGAG GCCAACATCT TCATGGTCCC CCAGAACATC
    AAGCCTGTTC TCCAGCGTAC TGCCATAGAG ATCCTGGCGT GGGGCCTGCG GAACATGAAG
    AGCTACCAGC TAGCCAGCAT CTCCTCCCCT AGCCTTGTGG TAGAGTGCGG GGGCCAGACA
    GTGCAGTCCT GTGTCATCAA GAACCTCCGG AAGAACCCCA ACTTCGACAT CTGTACCCTT
    TTCATGGAAG TGATGCTGCC CAAGGAGGAG GTCTACTGTC CCTCCATCGT GATCAAGGTC
    ATTGATAACC GCCAGTTTGG CCGCCGGCCT GTGGTGGGCC AGTGTACCAT CCGTTCCCTG
    GAGAGCTTCA TGTGTGACCT GTACTCCGAG GAGAATCCGT CCCCACAGGG CGGCCCAGAT
    GATGTGAGTT TACTCAGTCC TGGGGAAGAC GTGCTCATTG ACATCGATGA CAAGGAGCCC
    CTCATCCCCA TGCAGCTTGC AGATGGTCTG TCGGGCTCTG CCCCCACTAA CATGGCATCT
    TCTCCATCCA GTCCTCATAA GGCCCTCCTG GAGGAAGAGT TCATCGACTG GTGGAGCAAA
    TTCTTTGCCT CCACAGGGGA GAGGGAAAAG TGCGGCTCAT ACTTGGAGAA GGGCTTTGAC
    ACTCTAAAGG TCTATGACAC ACAACTGGAG AACGTGGAGG CCTTTGAGGG CCTGTCTGAC
    TTTTGTAACA CCTTCAAGCT CTACCGGGGC AAGACTCAGG AGGAGACAGA GGACCCATCT
    GTTATTGGGG AGTTTAAGGG CCTCTTCAAA ATCTATCCCC TCCCAGAAGA CCCGGCTGTT
    CCCATGCCCC CGAGACAATT CCACCAGCTG GCTGCCCAAG GGCCTCAGGA GTGCCTGGTC
    CGCATCTATA TCATCCGAGC ATTTGGCCTT CAGCCCAAGG ACCCCAATGG GAAGTGTGAT
    CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATTCCC
    TGTACTCTGG AGCCTGTGTT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG
    AAGGACCTGA AGATCACACT CTATGACTAT GACCTCCTCT CCAAAGATGA AAAGATTGGG
    GAGACAGTCA TTGACCTGGA GGATAGGCTG CTGTCCAAGT TTGGGGCTCG CTGCGGACTC
    CCACAGACGT ACTGTGTCTC TGGACCCAAC CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG
    CTTCTCCACC TCTTCTGCCA GCAGCGCAGA CTCAAGGCTC CTGTGTACCT CACGGACCAC
    GTGGTGTTCC AGGATAAAGA ATACAGCATT GAAGAAATAG AGGCTGGCCG AGTCCCGAAC
    CCACACCTGG GCCCAGTGGA AGAGCGTCTG GCCTTGCATG TCCTCCAGCA GCAGGGCCTG
    GTCCCTGAGC ATGTGGAGTC CCGGCCTCTC TACAGCCCCC TGCAGCCAGA CATTGAACAG
    GGGAAGCTGC AGATGTGGAT CGACCTATTT CCCAAGGCCC TGGGGCGTCC CGGACCTCCC
    TTCAACATCA CCCCGCGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATCAT CTGGAACACA
    AAGGACGTGA TCCTGGACGA CCTCAGCATC ACAGGGGAGA AGATGAGTGA TATTTATGTG
    AAGGGTTGGA TGGTTGGCTT TGAAGAGCAC AAGCAAAAGA CAGATGTGCA TTACCGGTCC
    CTGGGAGGTG AAGGCAACTT TAACTGGAGG TTCATTTTCC CCTTTGACTA CCTGCCAGCC
    GAGCAAGTCT GCACCGTCTC TAAGAAGGAT GCTTTCTGGA GGCTGGACAA GACTGAGAGC
    AAAATCCCAG CACGAGTGGT ATTTCAGATC TGGGACAATG ACAAGTTCTC CTTTGACGAC
    TTTCTGGGCT CCTTGCAGTT GGATCTCAAC CACATGCCCA AGCCTGCCAA GACAGCTGAG
    AAGTGCTCCT TGGACCAGCT GGATGACACT TTCCACCCGG AGTGGTTTGT GTCCCTTTTT
    GAACAGAAAA CAGTTAAGGG CTGGTGGCCC TGCGTAGCAG AAGAGGGCGA GAAGAAGATC
    CTGGCAGGCA AGCTGGAAAT GACCTTGGAG ATCGTAGCAG AGAGTGAGCA TGAGGAGCGG
    CCTGCTGGCC AGGGCCGGGA TGAACCCAAC ATGAACCCCA AGCTCGAGGA CCCAAGGCGC
    CCTGACACCT CCTTCCTCTG GTTCACCTCT CCATACAAGA CTATGAAGTT CATCCTGTGG
    CGGCGGTTCC GATGTGCCAT TATCCTCTTC ATCATTCTCT TCATCCTGCT ACTATTCTTA
    GGCATCTTCA TCTATGCTTT CCCGAACTAC GCTGCCATGA AGCTGGTGAA GCCCTTCAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022260557.1
    CDS23..6445
    Translation

    Target ORF information:

    RefSeq Version XM_022404849.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris dysferlin (DYSF), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022404849.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG
    CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC
    GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG
    AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT
    CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG
    ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT
    GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT
    TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC
    GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT
    ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT
    GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC
    GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC
    AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC
    AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC
    ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG
    GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT
    GCCCCTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTGG TCAAGCCTTC TCCAGCCACG
    GACCTGGATG ACCATCTGGG CTTCCTTCCC ACCTTCGGGC CCTGCTTCGT CAATCTCTAT
    GGCAGCCCCA GGGAATTCAC TGGCTTCCCG GACCCCTACG CGGAACTCAA CATGGGCAAG
    GGGGAAGGCG TGGCCTACCG AGGTCGGCTT CTGCTCTCCC TGGAGACCAA GCTGGTGGAG
    CACAGCGGCG AGCAAAGGGT GGAGGACCTC CCTGCTGATG ACATCCTTCG GGTGGAGAAA
    TACCTCCGGA GGCGCAAGTA CTCTCTCTTT GCCGCCTTCT ACTCGGCCAC CATGCTCCAG
    GATGTCGACG ATGCCATCCA GTTTGAGGTC AGCATTGGGA ACTATGGCAA CAAGTTCGAC
    ACGACCTGTC TGCCACTGGC CTCCACCACC CAGTACAGCC GGGCAGTCTT TGATGGGTGC
    CACTACTACT ACTTGCCCTG GGGCAATGTG AAGCCTGTGG TGGTGCTGTC ATCCTACTGG
    GAAGACATCA GCCACAGAGT CGAGGCTCAG AACCAGCTGC TCAGGATCGC TGACCATCTG
    GAAGCTGGCC TGGAGCAAGT CCACTTGGCT TTAAAGGCGC AGTTCAACAC AGAGGATGTG
    GACTCCCTGT TGGCCCAGCT GATGGATGAG CTCATCTCAG ACTGCAGCCA GCCCCTGGGC
    AATGTCCAAG AGATACCCTC TGCCACCCAC CTGGACCGGC ACCTGTACAG GCTGCGCACC
    CGTCACCTAG GCCAGATTAC TGAGGCGGCC CTGGCTCTGA AACTCGGGCA TGGCAAGCTC
    CCTGCCGCCC TGGAGCAGGC TGAAGACTGG CTCCTGCGTC TCCGTGCCCT GGCTGATGAG
    CCCCAGAACA GCCTGCCCGA CATCATCATC TGGATGCTGC AGGGAAACAA GCGAGTGGCG
    TACCAGCGGG TGCCTGCGCA CGAGGTCCTC TTCTCCCGGC GGGGCGCCAG CTACTGTGGC
    AAGAACTGTG GGAAGCTGCA GACGATATTT CTGAAATATC CCATGGAGGG GGTGCCCGGA
    GCCCGGATGC CAGTACAAAT ACGGGTGAAA CTCTGGTTTG GGCTTTCTGT GGATGAGAAG
    GAGTTCAACC AGTTTGCTGA GGGAAAGCTG TCCGTCTTTG CTGAAACTTA TGAGAACCAG
    ACCAAACTGG CCCTGGTTGG GAACTGGGGC ACAACAGGCC TCACCTACCC CAAGTTTTCT
    GATGTCATGG GCAAGATCAA GCTACCCAAG GATAGCTTCC GCCCCTCAGC TGGCTGGACC
    TGGGCCGGAG ACTGGTTCGT GTGTCCAGAG AAGACCCTAC TCCATGACAT GGACGCGGGC
    CACCTGAGTT TTGTGGAGGA GGTGTTTGAG AACCAGACCC GGCTCCCTGG AGGCCAGTGG
    ATCTACATGA GTGACAACTA CACAGATGTG AATGGGGATA AGGTGCTTCC TAAGGATGAC
    ATTGAGTGCC CACTGGGCTG GAAGTGGGAA GATGAAGAGT GGTCCACGGA CCTCAATCGG
    GCCGTCGATG AGCAAGGCTG GGAGTACAGT ATCACCATCC CCCCGGATCG GAAGCCAAAG
    CACTGGGTTC CTGCTGAGAA GATGTATTAC ACGCACCGTC GGCGGCGCTG GGTCCGCCTG
    CGCAGAAGGG ACCTCAGCCA GATGGAGGCA TTGAAGAGGC ACAGACAGGC AGAGGCAGAG
    GGTGAGGGCT GGGAGTACGC CTCGCTCTTG GGCTGGAAGT TCCACCTGGA GTACCGCAAG
    ACTGATGCTT TCCGCCGCCG CCGCTGGCGC CGCCGCATGG AACCGCTGGA GAAGACGGGG
    CCTGCAGCCG TGTTTGCCCT TGAGGGGGCT CTGGGTGGTG TGGTAGATGA CAGGAGTGAA
    GATTCCGTGT CCGTGTCCAC CTTGAGCTTT GGAGTGAACA GACCCACGAT TTCCTGCATC
    TTTGATTATG GGAACCGCTA CCACCTACGC TGCTACATGT ACCAGGCTCG GGACCTGCCC
    TCGATGGACA AGGACTCTTT TTCTGATCCC TACGCCATCG TCTCCTTCCT GCACCAGAGC
    CAGAAGACAG TGGTGGCGAA GAACACTCTG AACCCCACCT GGGACCAGAC GCTTATCTTC
    TATGAGATTG AGATCTTTGG TGAGCCAGCC AGTATCGCTG AGCAGCCGCC CAGCATTGTG
    GTGGAGCTGT ATGACCACGA CACCTACGGT GCAGATGAGT TTATGGGACG CTGCATTTGT
    CAGCCCAGCC TGGAGCGGAC ACCCCGGCTG GCCTGGTTCC CACTGATTAG GGGCAGCCAG
    TCGGCGGGGG AACTGCTGGC CTCTTTTGAG CTCATCCAGA GAGAAAAGCC GGCCATTCAC
    CATATTCCTG GTTTTGAGGT ACAGGATATG TCAGGAATCC TGGAAGAGTC AGAGGACATA
    GACCTGCCCT ACCCGCCACC TCAGAGGGAG GCCAACATCT TCATGGTCCC CCAGAACATC
    AAGCCTGTTC TCCAGCGTAC TGCCATAGAG ATCCTGGCGT GGGGCCTGCG GAACATGAAG
    AGCTACCAGC TAGCCAGCAT CTCCTCCCCT AGCCTTGTGG TAGAGTGCGG GGGCCAGACA
    GTGCAGTCCT GTGTCATCAA GAACCTCCGG AAGAACCCCA ACTTCGACAT CTGTACCCTT
    TTCATGGAAG TGATGCTGCC CAAGGAGGAG GTCTACTGTC CCTCCATCGT GATCAAGGTC
    ATTGATAACC GCCAGTTTGG CCGCCGGCCT GTGGTGGGCC AGTGTACCAT CCGTTCCCTG
    GAGAGCTTCA TGTGTGACCT GTACTCCGAG GAGAATCCGT CCCCACAGGG CGGCCCAGAT
    GATGTGAGTT TACTCAGTCC TGGGGAAGAC GTGCTCATTG ACATCGATGA CAAGGAGCCC
    CTCATCCCCA TGCAGCTTGC AGATGGTCTG TCGGGCTCTG CCCCCACTAA CATGGCATCT
    TCTCCATCCA GTCCTCATAA GGCCCTCCTG GAGGAAGAGT TCATCGACTG GTGGAGCAAA
    TTCTTTGCCT CCACAGGGGA GAGGGAAAAG TGCGGCTCAT ACTTGGAGAA GGGCTTTGAC
    ACTCTAAAGG TCTATGACAC ACAACTGGAG AACGTGGAGG CCTTTGAGGG CCTGTCTGAC
    TTTTGTAACA CCTTCAAGCT CTACCGGGGC AAGACTCAGG AGGAGACAGA GGACCCATCT
    GTTATTGGGG AGTTTAAGGG CCTCTTCAAA ATCTATCCCC TCCCAGAAGA CCCGGCTGTT
    CCCATGCCCC CGAGACAATT CCACCAGCTG GCTGCCCAAG GGCCTCAGGA GTGCCTGGTC
    CGCATCTATA TCATCCGAGC ATTTGGCCTT CAGCCCAAGG ACCCCAATGG GAAGTGTGAT
    CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATTCCC
    TGTACTCTGG AGCCTGTGTT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG
    AAGGACCTGA AGATCACACT CTATGACTAT GACCTCCTCT CCAAAGATGA AAAGATTGGG
    GAGACAGTCA TTGACCTGGA GGATAGGCTG CTGTCCAAGT TTGGGGCTCG CTGCGGACTC
    CCACAGACGT ACTGTGTCTC TGGACCCAAC CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG
    CTTCTCCACC TCTTCTGCCA GCAGCGCAGA CTCAAGGCTC CTGTGTACCT CACGGACCAC
    GTGGTGTTCC AGGATAAAGA ATACAGCATT GAAGAAATAG AGGCTGGCCG AGTCCCGAAC
    CCACACCTGG GCCCAGTGGA AGAGCGTCTG GCCTTGCATG TCCTCCAGCA GCAGGGCCTG
    GTCCCTGAGC ATGTGGAGTC CCGGCCTCTC TACAGCCCCC TGCAGCCAGA CATTGAACAG
    GGGAAGCTGC AGATGTGGAT CGACCTATTT CCCAAGGCCC TGGGGCGTCC CGGACCTCCC
    TTCAACATCA CCCCGCGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATCAT CTGGAACACA
    AAGGACGTGA TCCTGGACGA CCTCAGCATC ACAGGGGAGA AGATGAGTGA TATTTATGTG
    AAGGGTTGGA TGGTTGGCTT TGAAGAGCAC AAGCAAAAGA CAGATGTGCA TTACCGGTCC
    CTGGGAGGTG AAGGCAACTT TAACTGGAGG TTCATTTTCC CCTTTGACTA CCTGCCAGCC
    GAGCAAGTCT GCACCGTCTC TAAGAAGGAT GCTTTCTGGA GGCTGGACAA GACTGAGAGC
    AAAATCCCAG CACGAGTGGT ATTTCAGATC TGGGACAATG ACAAGTTCTC CTTTGACGAC
    TTTCTGGGCT CCTTGCAGTT GGATCTCAAC CACATGCCCA AGCCTGCCAA GACAGCTGAG
    AAGTGCTCCT TGGACCAGCT GGATGACACT TTCCACCCGG AGTGGTTTGT GTCCCTTTTT
    GAACAGAAAA CAGTTAAGGG CTGGTGGCCC TGCGTAGCAG AAGAGGGCGA GAAGAAGATC
    CTGGCAGGCA AGCTGGAAAT GACCTTGGAG ATCGTAGCAG AGAGTGAGCA TGAGGAGCGG
    CCTGCTGGCC AGGGCCGGGA TGAACCCAAC ATGAACCCCA AGCTCGAGGA CCCAAGGCGC
    CCTGACACCT CCTTCCTCTG GTTCACCTCT CCATACAAGA CTATGAAGTT CATCCTGTGG
    CGGCGGTTCC GATGTGCCAT TATCCTCTTC ATCATTCTCT TCATCCTGCT ACTATTCTTA
    GGCATCTTCA TCTATGCTTT CCCGAACTAC GCTGCCATGA AGCTGGTGAA GCCCTTCAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe294459
    Clone ID Related Accession (Same CDS sequence) XM_022404850.1
    Accession Version XM_022404850.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6423bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product dysferlin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG
    CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC
    GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG
    AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT
    CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG
    ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT
    GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT
    TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC
    GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT
    ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT
    GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC
    GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC
    AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC
    AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC
    ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG
    GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT
    GCCCCTGGAG GAGAAATAGA AGTGGATGAC CATCTGGGCT TCCTTCCCAC CTTCGGGCCC
    TGCTTCGTCA ATCTCTATGG CAGCCCCAGG GAATTCACTG GCTTCCCGGA CCCCTACGCG
    GAACTCAACA TGGGCAAGGG GGAAGGCGTG GCCTACCGAG GTCGGCTTCT GCTCTCCCTG
    GAGACCAAGC TGGTGGAGCA CAGCGGCGAG CAAAGGGTGG AGGACCTCCC TGCTGATGAC
    ATCCTTCGGG TGGAGAAATA CCTCCGGAGG CGCAAGTACT CTCTCTTTGC CGCCTTCTAC
    TCGGCCACCA TGCTCCAGGA TGTCGACGAT GCCATCCAGT TTGAGGTCAG CATTGGGAAC
    TATGGCAACA AGTTCGACAC GACCTGTCTG CCACTGGCCT CCACCACCCA GTACAGCCGG
    GCAGTCTTTG ATGGGTGCCA CTACTACTAC TTGCCCTGGG GCAATGTGAA GCCTGTGGTG
    GTGCTGTCAT CCTACTGGGA AGACATCAGC CACAGAGTCG AGGCTCAGAA CCAGCTGCTC
    AGGATCGCTG ACCATCTGGA AGCTGGCCTG GAGCAAGTCC ACTTGGCTTT AAAGGCGCAG
    TTCAACACAG AGGATGTGGA CTCCCTGTTG GCCCAGCTGA TGGATGAGCT CATCTCAGAC
    TGCAGCCAGC CCCTGGGCAA TGTCCAAGAG ATACCCTCTG CCACCCACCT GGACCGGCAC
    CTGTACAGGC TGCGCACCCG TCACCTAGGC CAGATTACTG AGGCGGCCCT GGCTCTGAAA
    CTCGGGCATG GCAAGCTCCC TGCCGCCCTG GAGCAGGCTG AAGACTGGCT CCTGCGTCTC
    CGTGCCCTGG CTGATGAGCC CCAGAACAGC CTGCCCGACA TCATCATCTG GATGCTGCAG
    GGAAACAAGC GAGTGGCGTA CCAGCGGGTG CCTGCGCACG AGGTCCTCTT CTCCCGGCGG
    GGCGCCAGCT ACTGTGGCAA GAACTGTGGG AAGCTGCAGA CGATATTTCT GAAATATCCC
    ATGGAGGGGG TGCCCGGAGC CCGGATGCCA GTACAAATAC GGGTGAAACT CTGGTTTGGG
    CTTTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GAAAGCTGTC CGTCTTTGCT
    GAAACTTATG AGAACCAGAC CAAACTGGCC CTGGTTGGGA ACTGGGGCAC AACAGGCCTC
    ACCTACCCCA AGTTTTCTGA TGTCATGGGC AAGATCAAGC TACCCAAGGA TAGCTTCCGC
    CCCTCAGCTG GCTGGACCTG GGCCGGAGAC TGGTTCGTGT GTCCAGAGAA GACCCTACTC
    CATGACATGG ACGCGGGCCA CCTGAGTTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG
    CTCCCTGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CAGATGTGAA TGGGGATAAG
    GTGCTTCCTA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAAGAGTGG
    TCCACGGACC TCAATCGGGC CGTCGATGAG CAAGGCTGGG AGTACAGTAT CACCATCCCC
    CCGGATCGGA AGCCAAAGCA CTGGGTTCCT GCTGAGAAGA TGTATTACAC GCACCGTCGG
    CGGCGCTGGG TCCGCCTGCG CAGAAGGGAC CTCAGCCAGA TGGAGGCATT GAAGAGGCAC
    AGACAGGCAG AGGCAGAGGG TGAGGGCTGG GAGTACGCCT CGCTCTTGGG CTGGAAGTTC
    CACCTGGAGT ACCGCAAGAC TGATGCTTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAA
    CCGCTGGAGA AGACGGGGCC TGCAGCCGTG TTTGCCCTTG AGGGGGCTCT GGGTGGTGTG
    GTAGATGACA GGAGTGAAGA TTCCGTGTCC GTGTCCACCT TGAGCTTTGG AGTGAACAGA
    CCCACGATTT CCTGCATCTT TGATTATGGG AACCGCTACC ACCTACGCTG CTACATGTAC
    CAGGCTCGGG ACCTGCCCTC GATGGACAAG GACTCTTTTT CTGATCCCTA CGCCATCGTC
    TCCTTCCTGC ACCAGAGCCA GAAGACAGTG GTGGCGAAGA ACACTCTGAA CCCCACCTGG
    GACCAGACGC TTATCTTCTA TGAGATTGAG ATCTTTGGTG AGCCAGCCAG TATCGCTGAG
    CAGCCGCCCA GCATTGTGGT GGAGCTGTAT GACCACGACA CCTACGGTGC AGATGAGTTT
    ATGGGACGCT GCATTTGTCA GCCCAGCCTG GAGCGGACAC CCCGGCTGGC CTGGTTCCCA
    CTGATTAGGG GCAGCCAGTC GGCGGGGGAA CTGCTGGCCT CTTTTGAGCT CATCCAGAGA
    GAAAAGCCGG CCATTCACCA TATTCCTGGT TTTGAGGTAC AGGATATGTC AGGAATCCTG
    GAAGAGCTGT GTGCACCTGC CTTCTTCCTC CAGGGCCTCC TCCAAACGTC AGAGGACATA
    GACCTGCCCT ACCCGCCACC TCAGAGGGAG GCCAACATCT TCATGGTCCC CCAGAACATC
    AAGCCTGTTC TCCAGCGTAC TGCCATAGAG ATCCTGGCGT GGGGCCTGCG GAACATGAAG
    AGCTACCAGC TAGCCAGCAT CTCCTCCCCT AGCCTTGTGG TAGAGTGCGG GGGCCAGACA
    GTGCAGTCCT GTGTCATCAA GAACCTCCGG AAGAACCCCA ACTTCGACAT CTGTACCCTT
    TTCATGGAAG TGATGCTGCC CAAGGAGGAG GTCTACTGTC CCTCCATCGT GATCAAGGTC
    ATTGATAACC GCCAGTTTGG CCGCCGGCCT GTGGTGGGCC AGTGTACCAT CCGTTCCCTG
    GAGAGCTTCA TGTGTGACCT GTACTCCGAG GAGAATCCGT CCCCACAGGG CGGCCCAGAT
    GATGTGAGTT TACTCAGTCC TGGGGAAGAC GTGCTCATTG ACATCGATGA CAAGGAGCCC
    CTCATCCCCA TGCAGCTTGC AGATGGTCTG TCGGGCTCTG CCCCCACTAA CATGGCATCT
    TCTCCATCCA GTCCTCATAA GGCCCTCCTG GAGGAAGAGT TCATCGACTG GTGGAGCAAA
    TTCTTTGCCT CCACAGGGGA GAGGGAAAAG TGCGGCTCAT ACTTGGAGAA GGGCTTTGAC
    ACTCTAAAGG TCTATGACAC ACAACTGGAG AACGTGGAGG CCTTTGAGGG CCTGTCTGAC
    TTTTGTAACA CCTTCAAGCT CTACCGGGGC AAGACTCAGG AGGAGACAGA GGACCCATCT
    GTTATTGGGG AGTTTAAGGG CCTCTTCAAA ATCTATCCCC TCCCAGAAGA CCCGGCTGTT
    CCCATGCCCC CGAGACAATT CCACCAGCTG GCTGCCCAAG GGCCTCAGGA GTGCCTGGTC
    CGCATCTATA TCATCCGAGC ATTTGGCCTT CAGCCCAAGG ACCCCAATGG GAAGTGTGAT
    CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATTCCC
    TGTACTCTGG AGCCTGTGTT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG
    AAGGACCTGA AGATCACACT CTATGACTAT GACCTCCTCT CCAAAGATGA AAAGATTGGG
    GAGACAGTCA TTGACCTGGA GGATAGGCTG CTGTCCAAGT TTGGGGCTCG CTGCGGACTC
    CCACAGACGT ACTGTGTCTC TGGACCCAAC CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG
    CTTCTCCACC TCTTCTGCCA GCAGCGCAGA CTCAAGGCTC CTGTGTACCT CACGGACCAC
    GTGGTGTTCC AGGATAAAGA ATACAGCATT GAAGAAATAG AGGCTGGCCG AGTCCCGAAC
    CCACACCTGG GCCCAGTGGA AGAGCGTCTG GCCTTGCATG TCCTCCAGCA GCAGGGCCTG
    GTCCCTGAGC ATGTGGAGTC CCGGCCTCTC TACAGCCCCC TGCAGCCAGA CATTGAACAG
    GGGAAGCTGC AGATGTGGAT CGACCTATTT CCCAAGGCCC TGGGGCGTCC CGGACCTCCC
    TTCAACATCA CCCCGCGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATCAT CTGGAACACA
    AAGGACGTGA TCCTGGACGA CCTCAGCATC ACAGGGGAGA AGATGAGTGA TATTTATGTG
    AAGGGTTGGA TGGTTGGCTT TGAAGAGCAC AAGCAAAAGA CAGATGTGCA TTACCGGTCC
    CTGGGAGGTG AAGGCAACTT TAACTGGAGG TTCATTTTCC CCTTTGACTA CCTGCCAGCC
    GAGCAAGTCT GCACCGTCTC TAAGAAGGAT GCTTTCTGGA GGCTGGACAA GACTGAGAGC
    AAAATCCCAG CACGAGTGGT ATTTCAGATC TGGGACAATG ACAAGTTCTC CTTTGACGAC
    TTTCTGGGCT CCTTGCAGTT GGATCTCAAC CACATGCCCA AGCCTGCCAA GACAGCTGAG
    AAGTGCTCCT TGGACCAGCT GGATGACACT TTCCACCCGG AGTGGTTTGT GTCCCTTTTT
    GAACAGAAAA CAGTTAAGGG CTGGTGGCCC TGCGTAGCAG AAGAGGGCGA GAAGAAGATC
    CTGGCAGGCA AGCTGGAAAT GACCTTGGAG ATCGTAGCAG AGAGTGAGCA TGAGGAGCGG
    CCTGCTGGCC AGGGCCGGGA TGAACCCAAC ATGAACCCCA AGCTCGAGGA CCCAAGGCGC
    CCTGACACCT CCTTCCTCTG GTTCACCTCT CCATACAAGA CTATGAAGTT CATCCTGTGG
    CGGCGGTTCC GATGTGCCAT TATCCTCTTC ATCATTCTCT TCATCCTGCT ACTATTCTTA
    GGCATCTTCA TCTATGCTTT CCCGAACTAC GCTGCCATGA AGCTGGTGAA GCCCTTCAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022260558.1
    CDS23..6445
    Translation

    Target ORF information:

    RefSeq Version XM_022404850.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris dysferlin (DYSF), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022404850.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG
    CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC
    GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG
    AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT
    CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG
    ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT
    GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT
    TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC
    GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT
    ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT
    GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC
    GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC
    AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC
    AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC
    ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG
    GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT
    GCCCCTGGAG GAGAAATAGA AGTGGATGAC CATCTGGGCT TCCTTCCCAC CTTCGGGCCC
    TGCTTCGTCA ATCTCTATGG CAGCCCCAGG GAATTCACTG GCTTCCCGGA CCCCTACGCG
    GAACTCAACA TGGGCAAGGG GGAAGGCGTG GCCTACCGAG GTCGGCTTCT GCTCTCCCTG
    GAGACCAAGC TGGTGGAGCA CAGCGGCGAG CAAAGGGTGG AGGACCTCCC TGCTGATGAC
    ATCCTTCGGG TGGAGAAATA CCTCCGGAGG CGCAAGTACT CTCTCTTTGC CGCCTTCTAC
    TCGGCCACCA TGCTCCAGGA TGTCGACGAT GCCATCCAGT TTGAGGTCAG CATTGGGAAC
    TATGGCAACA AGTTCGACAC GACCTGTCTG CCACTGGCCT CCACCACCCA GTACAGCCGG
    GCAGTCTTTG ATGGGTGCCA CTACTACTAC TTGCCCTGGG GCAATGTGAA GCCTGTGGTG
    GTGCTGTCAT CCTACTGGGA AGACATCAGC CACAGAGTCG AGGCTCAGAA CCAGCTGCTC
    AGGATCGCTG ACCATCTGGA AGCTGGCCTG GAGCAAGTCC ACTTGGCTTT AAAGGCGCAG
    TTCAACACAG AGGATGTGGA CTCCCTGTTG GCCCAGCTGA TGGATGAGCT CATCTCAGAC
    TGCAGCCAGC CCCTGGGCAA TGTCCAAGAG ATACCCTCTG CCACCCACCT GGACCGGCAC
    CTGTACAGGC TGCGCACCCG TCACCTAGGC CAGATTACTG AGGCGGCCCT GGCTCTGAAA
    CTCGGGCATG GCAAGCTCCC TGCCGCCCTG GAGCAGGCTG AAGACTGGCT CCTGCGTCTC
    CGTGCCCTGG CTGATGAGCC CCAGAACAGC CTGCCCGACA TCATCATCTG GATGCTGCAG
    GGAAACAAGC GAGTGGCGTA CCAGCGGGTG CCTGCGCACG AGGTCCTCTT CTCCCGGCGG
    GGCGCCAGCT ACTGTGGCAA GAACTGTGGG AAGCTGCAGA CGATATTTCT GAAATATCCC
    ATGGAGGGGG TGCCCGGAGC CCGGATGCCA GTACAAATAC GGGTGAAACT CTGGTTTGGG
    CTTTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GAAAGCTGTC CGTCTTTGCT
    GAAACTTATG AGAACCAGAC CAAACTGGCC CTGGTTGGGA ACTGGGGCAC AACAGGCCTC
    ACCTACCCCA AGTTTTCTGA TGTCATGGGC AAGATCAAGC TACCCAAGGA TAGCTTCCGC
    CCCTCAGCTG GCTGGACCTG GGCCGGAGAC TGGTTCGTGT GTCCAGAGAA GACCCTACTC
    CATGACATGG ACGCGGGCCA CCTGAGTTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG
    CTCCCTGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CAGATGTGAA TGGGGATAAG
    GTGCTTCCTA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAAGAGTGG
    TCCACGGACC TCAATCGGGC CGTCGATGAG CAAGGCTGGG AGTACAGTAT CACCATCCCC
    CCGGATCGGA AGCCAAAGCA CTGGGTTCCT GCTGAGAAGA TGTATTACAC GCACCGTCGG
    CGGCGCTGGG TCCGCCTGCG CAGAAGGGAC CTCAGCCAGA TGGAGGCATT GAAGAGGCAC
    AGACAGGCAG AGGCAGAGGG TGAGGGCTGG GAGTACGCCT CGCTCTTGGG CTGGAAGTTC
    CACCTGGAGT ACCGCAAGAC TGATGCTTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAA
    CCGCTGGAGA AGACGGGGCC TGCAGCCGTG TTTGCCCTTG AGGGGGCTCT GGGTGGTGTG
    GTAGATGACA GGAGTGAAGA TTCCGTGTCC GTGTCCACCT TGAGCTTTGG AGTGAACAGA
    CCCACGATTT CCTGCATCTT TGATTATGGG AACCGCTACC ACCTACGCTG CTACATGTAC
    CAGGCTCGGG ACCTGCCCTC GATGGACAAG GACTCTTTTT CTGATCCCTA CGCCATCGTC
    TCCTTCCTGC ACCAGAGCCA GAAGACAGTG GTGGCGAAGA ACACTCTGAA CCCCACCTGG
    GACCAGACGC TTATCTTCTA TGAGATTGAG ATCTTTGGTG AGCCAGCCAG TATCGCTGAG
    CAGCCGCCCA GCATTGTGGT GGAGCTGTAT GACCACGACA CCTACGGTGC AGATGAGTTT
    ATGGGACGCT GCATTTGTCA GCCCAGCCTG GAGCGGACAC CCCGGCTGGC CTGGTTCCCA
    CTGATTAGGG GCAGCCAGTC GGCGGGGGAA CTGCTGGCCT CTTTTGAGCT CATCCAGAGA
    GAAAAGCCGG CCATTCACCA TATTCCTGGT TTTGAGGTAC AGGATATGTC AGGAATCCTG
    GAAGAGCTGT GTGCACCTGC CTTCTTCCTC CAGGGCCTCC TCCAAACGTC AGAGGACATA
    GACCTGCCCT ACCCGCCACC TCAGAGGGAG GCCAACATCT TCATGGTCCC CCAGAACATC
    AAGCCTGTTC TCCAGCGTAC TGCCATAGAG ATCCTGGCGT GGGGCCTGCG GAACATGAAG
    AGCTACCAGC TAGCCAGCAT CTCCTCCCCT AGCCTTGTGG TAGAGTGCGG GGGCCAGACA
    GTGCAGTCCT GTGTCATCAA GAACCTCCGG AAGAACCCCA ACTTCGACAT CTGTACCCTT
    TTCATGGAAG TGATGCTGCC CAAGGAGGAG GTCTACTGTC CCTCCATCGT GATCAAGGTC
    ATTGATAACC GCCAGTTTGG CCGCCGGCCT GTGGTGGGCC AGTGTACCAT CCGTTCCCTG
    GAGAGCTTCA TGTGTGACCT GTACTCCGAG GAGAATCCGT CCCCACAGGG CGGCCCAGAT
    GATGTGAGTT TACTCAGTCC TGGGGAAGAC GTGCTCATTG ACATCGATGA CAAGGAGCCC
    CTCATCCCCA TGCAGCTTGC AGATGGTCTG TCGGGCTCTG CCCCCACTAA CATGGCATCT
    TCTCCATCCA GTCCTCATAA GGCCCTCCTG GAGGAAGAGT TCATCGACTG GTGGAGCAAA
    TTCTTTGCCT CCACAGGGGA GAGGGAAAAG TGCGGCTCAT ACTTGGAGAA GGGCTTTGAC
    ACTCTAAAGG TCTATGACAC ACAACTGGAG AACGTGGAGG CCTTTGAGGG CCTGTCTGAC
    TTTTGTAACA CCTTCAAGCT CTACCGGGGC AAGACTCAGG AGGAGACAGA GGACCCATCT
    GTTATTGGGG AGTTTAAGGG CCTCTTCAAA ATCTATCCCC TCCCAGAAGA CCCGGCTGTT
    CCCATGCCCC CGAGACAATT CCACCAGCTG GCTGCCCAAG GGCCTCAGGA GTGCCTGGTC
    CGCATCTATA TCATCCGAGC ATTTGGCCTT CAGCCCAAGG ACCCCAATGG GAAGTGTGAT
    CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATTCCC
    TGTACTCTGG AGCCTGTGTT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG
    AAGGACCTGA AGATCACACT CTATGACTAT GACCTCCTCT CCAAAGATGA AAAGATTGGG
    GAGACAGTCA TTGACCTGGA GGATAGGCTG CTGTCCAAGT TTGGGGCTCG CTGCGGACTC
    CCACAGACGT ACTGTGTCTC TGGACCCAAC CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG
    CTTCTCCACC TCTTCTGCCA GCAGCGCAGA CTCAAGGCTC CTGTGTACCT CACGGACCAC
    GTGGTGTTCC AGGATAAAGA ATACAGCATT GAAGAAATAG AGGCTGGCCG AGTCCCGAAC
    CCACACCTGG GCCCAGTGGA AGAGCGTCTG GCCTTGCATG TCCTCCAGCA GCAGGGCCTG
    GTCCCTGAGC ATGTGGAGTC CCGGCCTCTC TACAGCCCCC TGCAGCCAGA CATTGAACAG
    GGGAAGCTGC AGATGTGGAT CGACCTATTT CCCAAGGCCC TGGGGCGTCC CGGACCTCCC
    TTCAACATCA CCCCGCGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATCAT CTGGAACACA
    AAGGACGTGA TCCTGGACGA CCTCAGCATC ACAGGGGAGA AGATGAGTGA TATTTATGTG
    AAGGGTTGGA TGGTTGGCTT TGAAGAGCAC AAGCAAAAGA CAGATGTGCA TTACCGGTCC
    CTGGGAGGTG AAGGCAACTT TAACTGGAGG TTCATTTTCC CCTTTGACTA CCTGCCAGCC
    GAGCAAGTCT GCACCGTCTC TAAGAAGGAT GCTTTCTGGA GGCTGGACAA GACTGAGAGC
    AAAATCCCAG CACGAGTGGT ATTTCAGATC TGGGACAATG ACAAGTTCTC CTTTGACGAC
    TTTCTGGGCT CCTTGCAGTT GGATCTCAAC CACATGCCCA AGCCTGCCAA GACAGCTGAG
    AAGTGCTCCT TGGACCAGCT GGATGACACT TTCCACCCGG AGTGGTTTGT GTCCCTTTTT
    GAACAGAAAA CAGTTAAGGG CTGGTGGCCC TGCGTAGCAG AAGAGGGCGA GAAGAAGATC
    CTGGCAGGCA AGCTGGAAAT GACCTTGGAG ATCGTAGCAG AGAGTGAGCA TGAGGAGCGG
    CCTGCTGGCC AGGGCCGGGA TGAACCCAAC ATGAACCCCA AGCTCGAGGA CCCAAGGCGC
    CCTGACACCT CCTTCCTCTG GTTCACCTCT CCATACAAGA CTATGAAGTT CATCCTGTGG
    CGGCGGTTCC GATGTGCCAT TATCCTCTTC ATCATTCTCT TCATCCTGCT ACTATTCTTA
    GGCATCTTCA TCTATGCTTT CCCGAACTAC GCTGCCATGA AGCTGGTGAA GCCCTTCAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe294460
    Clone ID Related Accession (Same CDS sequence) XM_022404851.1
    Accession Version XM_022404851.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6390bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product dysferlin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG
    CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC
    GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG
    AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT
    CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG
    ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT
    GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT
    TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC
    GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT
    ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT
    GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC
    GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC
    AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC
    AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC
    ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG
    GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT
    GCCCCTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTGG TCAAGCCTTC TCCAGCCACG
    GACCTGGATG ACCATCTGGG CTTCCTTCCC ACCTTCGGGC CCTGCTTCGT CAATCTCTAT
    GGCAGCCCCA GGGAATTCAC TGGCTTCCCG GACCCCTACG CGGAACTCAA CATGGGCAAG
    GGGGAAGGCG TGGCCTACCG AGGTCGGCTT CTGCTCTCCC TGGAGACCAA GCTGGTGGAG
    CACAGCGGCG AGCAAAGGGT GGAGGACCTC CCTGCTGATG ACATCCTTCG GGTGGAGAAA
    TACCTCCGGA GGCGCAAGTA CTCTCTCTTT GCCGCCTTCT ACTCGGCCAC CATGCTCCAG
    GATGTCGACG ATGCCATCCA GTTTGAGGTC AGCATTGGGA ACTATGGCAA CAAGTTCGAC
    ACGACCTGTC TGCCACTGGC CTCCACCACC CAGTACAGCC GGGCAGTCTT TGATGGGTGC
    CACTACTACT ACTTGCCCTG GGGCAATGTG AAGCCTGTGG TGGTGCTGTC ATCCTACTGG
    GAAGACATCA GCCACAGAGT CGAGGCTCAG AACCAGCTGC TCAGGATCGC TGACCATCTG
    GAAGCTGGCC TGGAGCAAGT CCACTTGGCT TTAAAGGCGC AGTTCAACAC AGAGGATGTG
    GACTCCCTGT TGGCCCAGCT GATGGATGAG CTCATCTCAG ACTGCAGCCA GCCCCTGGGC
    AATGTCCAAG AGATACCCTC TGCCACCCAC CTGGACCGGC ACCTGTACAG GCTGCGCACC
    CGTCACCTAG GCCAGATTAC TGAGGCGGCC CTGGCTCTGA AACTCGGGCA TGGCAAGCTC
    CCTGCCGCCC TGGAGCAGGC TGAAGACTGG CTCCTGCGTC TCCGTGCCCT GGCTGATGAG
    CCCCAGAACA GCCTGCCCGA CATCATCATC TGGATGCTGC AGGGAAACAA GCGAGTGGCG
    TACCAGCGGG TGCCTGCGCA CGAGGTCCTC TTCTCCCGGC GGGGCGCCAG CTACTGTGGC
    AAGAACTGTG GGAAGCTGCA GACGATATTT CTGAAATATC CCATGGAGGG GGTGCCCGGA
    GCCCGGATGC CAGTACAAAT ACGGGTGAAA CTCTGGTTTG GGCTTTCTGT GGATGAGAAG
    GAGTTCAACC AGTTTGCTGA GGGAAAGCTG TCCGTCTTTG CTGAAACTTA TGAGAACCAG
    ACCAAACTGG CCCTGGTTGG GAACTGGGGC ACAACAGGCC TCACCTACCC CAAGTTTTCT
    GATGTCATGG GCAAGATCAA GCTACCCAAG GATAGCTTCC GCCCCTCAGC TGGCTGGACC
    TGGGCCGGAG ACTGGTTCGT GTGTCCAGAG AAGACCCTAC TCCATGACAT GGACGCGGGC
    CACCTGAGTT TTGTGGAGGA GGTGTTTGAG AACCAGACCC GGCTCCCTGG AGGCCAGTGG
    ATCTACATGA GTGACAACTA CACAGATGTG AATGGGGATA AGGTGCTTCC TAAGGATGAC
    ATTGAGTGCC CACTGGGCTG GAAGTGGGAA GATGAAGAGT GGTCCACGGA CCTCAATCGG
    GCCGTCGATG AGCAAGGCTG GGAGTACAGT ATCACCATCC CCCCGGATCG GAAGCCAAAG
    CACTGGGTTC CTGCTGAGAA GATGTATTAC ACGCACCGTC GGCGGCGCTG GGTCCGCCTG
    CGCAGAAGGG ACCTCAGCCA GATGGAGGCA TTGAAGAGGC ACAGACAGGC AGAGGCAGAG
    GGTGAGGGCT GGGAGTACGC CTCGCTCTTG GGCTGGAAGT TCCACCTGGA GTACCGCAAG
    ACTGATGCTT TCCGCCGCCG CCGCTGGCGC CGCCGCATGG AACCGCTGGA GAAGACGGGG
    CCTGCAGCCG TGTTTGCCCT TGAGGGGGCT CTGGGTGGTG TGGTAGATGA CAGGAGTGAA
    GATTCCGTGT CCGTGTCCAC CTTGAGCTTT GGAGTGAACA GACCCACGAT TTCCTGCATC
    TTTGATTATG GGAACCGCTA CCACCTACGC TGCTACATGT ACCAGGCTCG GGACCTGCCC
    TCGATGGACA AGGACTCTTT TTCTGATCCC TACGCCATCG TCTCCTTCCT GCACCAGAGC
    CAGAAGACAG TGGTGGCGAA GAACACTCTG AACCCCACCT GGGACCAGAC GCTTATCTTC
    TATGAGATTG AGATCTTTGG TGAGCCAGCC AGTATCGCTG AGCAGCCGCC CAGCATTGTG
    GTGGAGCTGT ATGACCACGA CACCTACGGT GCAGATGAGT TTATGGGACG CTGCATTTGT
    CAGCCCAGCC TGGAGCGGAC ACCCCGGCTG GCCTGGTTCC CACTGATTAG GGGCAGCCAG
    TCGGCGGGGG AACTGCTGGC CTCTTTTGAG CTCATCCAGA GAGAAAAGCC GGCCATTCAC
    CATATTCCTG GTTTTGAGGT ACAGGATATG TCAGGAATCC TGGAAGAGCT GTGTGCACCT
    GCCTTCTTCC TCCAGGGCCT CCTCCAAACG TCAGAGGACA TAGACCTGCC CTACCCGCCA
    CCTCAGAGGG AGGCCAACAT CTTCATGGTC CCCCAGAACA TCAAGCCTGT TCTCCAGCGT
    ACTGCCATAG AGATCCTGGC GTGGGGCCTG CGGAACATGA AGAGCTACCA GCTAGCCAGC
    ATCTCCTCCC CTAGCCTTGT GGTAGAGTGC GGGGGCCAGA CAGTGCAGTC CTGTGTCATC
    AAGAACCTCC GGAAGAACCC CAACTTCGAC ATCTGTACCC TTTTCATGGA AGTGATGCTG
    CCCAAGGAGG AGGTCTACTG TCCCTCCATC GTGATCAAGG TCATTGATAA CCGCCAGTTT
    GGCCGCCGGC CTGTGGTGGG CCAGTGTACC ATCCGTTCCC TGGAGAGCTT CATGTGTGAC
    CTGTACTCCG AGGAGAATCC GTCCCCACAG GGCGGCCCAG ATGATGTGAG TTTACTCAGT
    CCTGGGGAAG ACGTGCTCAT TGACATCGAT GACAAGGAGC CCCTCATCCC CATGCAGGAG
    GAAGAGTTCA TCGACTGGTG GAGCAAATTC TTTGCCTCCA CAGGGGAGAG GGAAAAGTGC
    GGCTCATACT TGGAGAAGGG CTTTGACACT CTAAAGGTCT ATGACACACA ACTGGAGAAC
    GTGGAGGCCT TTGAGGGCCT GTCTGACTTT TGTAACACCT TCAAGCTCTA CCGGGGCAAG
    ACTCAGGAGG AGACAGAGGA CCCATCTGTT ATTGGGGAGT TTAAGGGCCT CTTCAAAATC
    TATCCCCTCC CAGAAGACCC GGCTGTTCCC ATGCCCCCGA GACAATTCCA CCAGCTGGCT
    GCCCAAGGGC CTCAGGAGTG CCTGGTCCGC ATCTATATCA TCCGAGCATT TGGCCTTCAG
    CCCAAGGACC CCAATGGGAA GTGTGATCCT TACATCAAGA TCTCCATAGG GAAGAAATCA
    GTGAGTGACC AGGATAACTA CATTCCCTGT ACTCTGGAGC CTGTGTTTGG AAAGATGTTC
    GAGCTGACCT GCACTCTGCC TCTGGAGAAG GACCTGAAGA TCACACTCTA TGACTATGAC
    CTCCTCTCCA AAGATGAAAA GATTGGGGAG ACAGTCATTG ACCTGGAGGA TAGGCTGCTG
    TCCAAGTTTG GGGCTCGCTG CGGACTCCCA CAGACGTACT GTGTCTCTGG ACCCAACCAG
    TGGAGGGACC AGCTCCGCCC CTCCCAGCTT CTCCACCTCT TCTGCCAGCA GCGCAGACTC
    AAGGCTCCTG TGTACCTCAC GGACCACGTG GTGTTCCAGG ATAAAGAATA CAGCATTGAA
    GAAATAGAGG CTGGCCGAGT CCCGAACCCA CACCTGGGCC CAGTGGAAGA GCGTCTGGCC
    TTGCATGTCC TCCAGCAGCA GGGCCTGGTC CCTGAGCATG TGGAGTCCCG GCCTCTCTAC
    AGCCCCCTGC AGCCAGACAT TGAACAGGGG AAGCTGCAGA TGTGGATCGA CCTATTTCCC
    AAGGCCCTGG GGCGTCCCGG ACCTCCCTTC AACATCACCC CGCGGAGAGC CAGAAGGTTT
    TTCCTGCGTT GTATCATCTG GAACACAAAG GACGTGATCC TGGACGACCT CAGCATCACA
    GGGGAGAAGA TGAGTGATAT TTATGTGAAG GGTTGGATGG TTGGCTTTGA AGAGCACAAG
    CAAAAGACAG ATGTGCATTA CCGGTCCCTG GGAGGTGAAG GCAACTTTAA CTGGAGGTTC
    ATTTTCCCCT TTGACTACCT GCCAGCCGAG CAAGTCTGCA CCGTCTCTAA GAAGGATGCT
    TTCTGGAGGC TGGACAAGAC TGAGAGCAAA ATCCCAGCAC GAGTGGTATT TCAGATCTGG
    GACAATGACA AGTTCTCCTT TGACGACTTT CTGGGCTCCT TGCAGTTGGA TCTCAACCAC
    ATGCCCAAGC CTGCCAAGAC AGCTGAGAAG TGCTCCTTGG ACCAGCTGGA TGACACTTTC
    CACCCGGAGT GGTTTGTGTC CCTTTTTGAA CAGAAAACAG TTAAGGGCTG GTGGCCCTGC
    GTAGCAGAAG AGGGCGAGAA GAAGATCCTG GCAGGCAAGC TGGAAATGAC CTTGGAGATC
    GTAGCAGAGA GTGAGCATGA GGAGCGGCCT GCTGGCCAGG GCCGGGATGA ACCCAACATG
    AACCCCAAGC TCGAGGACCC AAGGCGCCCT GACACCTCCT TCCTCTGGTT CACCTCTCCA
    TACAAGACTA TGAAGTTCAT CCTGTGGCGG CGGTTCCGAT GTGCCATTAT CCTCTTCATC
    ATTCTCTTCA TCCTGCTACT ATTCTTAGGC ATCTTCATCT ATGCTTTCCC GAACTACGCT
    GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022260559.1
    CDS23..6412
    Translation

    Target ORF information:

    RefSeq Version XM_022404851.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris dysferlin (DYSF), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022404851.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG
    CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC
    GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG
    AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT
    CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG
    ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT
    GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT
    TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC
    GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT
    ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT
    GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC
    GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC
    AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC
    AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC
    ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG
    GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT
    GCCCCTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTGG TCAAGCCTTC TCCAGCCACG
    GACCTGGATG ACCATCTGGG CTTCCTTCCC ACCTTCGGGC CCTGCTTCGT CAATCTCTAT
    GGCAGCCCCA GGGAATTCAC TGGCTTCCCG GACCCCTACG CGGAACTCAA CATGGGCAAG
    GGGGAAGGCG TGGCCTACCG AGGTCGGCTT CTGCTCTCCC TGGAGACCAA GCTGGTGGAG
    CACAGCGGCG AGCAAAGGGT GGAGGACCTC CCTGCTGATG ACATCCTTCG GGTGGAGAAA
    TACCTCCGGA GGCGCAAGTA CTCTCTCTTT GCCGCCTTCT ACTCGGCCAC CATGCTCCAG
    GATGTCGACG ATGCCATCCA GTTTGAGGTC AGCATTGGGA ACTATGGCAA CAAGTTCGAC
    ACGACCTGTC TGCCACTGGC CTCCACCACC CAGTACAGCC GGGCAGTCTT TGATGGGTGC
    CACTACTACT ACTTGCCCTG GGGCAATGTG AAGCCTGTGG TGGTGCTGTC ATCCTACTGG
    GAAGACATCA GCCACAGAGT CGAGGCTCAG AACCAGCTGC TCAGGATCGC TGACCATCTG
    GAAGCTGGCC TGGAGCAAGT CCACTTGGCT TTAAAGGCGC AGTTCAACAC AGAGGATGTG
    GACTCCCTGT TGGCCCAGCT GATGGATGAG CTCATCTCAG ACTGCAGCCA GCCCCTGGGC
    AATGTCCAAG AGATACCCTC TGCCACCCAC CTGGACCGGC ACCTGTACAG GCTGCGCACC
    CGTCACCTAG GCCAGATTAC TGAGGCGGCC CTGGCTCTGA AACTCGGGCA TGGCAAGCTC
    CCTGCCGCCC TGGAGCAGGC TGAAGACTGG CTCCTGCGTC TCCGTGCCCT GGCTGATGAG
    CCCCAGAACA GCCTGCCCGA CATCATCATC TGGATGCTGC AGGGAAACAA GCGAGTGGCG
    TACCAGCGGG TGCCTGCGCA CGAGGTCCTC TTCTCCCGGC GGGGCGCCAG CTACTGTGGC
    AAGAACTGTG GGAAGCTGCA GACGATATTT CTGAAATATC CCATGGAGGG GGTGCCCGGA
    GCCCGGATGC CAGTACAAAT ACGGGTGAAA CTCTGGTTTG GGCTTTCTGT GGATGAGAAG
    GAGTTCAACC AGTTTGCTGA GGGAAAGCTG TCCGTCTTTG CTGAAACTTA TGAGAACCAG
    ACCAAACTGG CCCTGGTTGG GAACTGGGGC ACAACAGGCC TCACCTACCC CAAGTTTTCT
    GATGTCATGG GCAAGATCAA GCTACCCAAG GATAGCTTCC GCCCCTCAGC TGGCTGGACC
    TGGGCCGGAG ACTGGTTCGT GTGTCCAGAG AAGACCCTAC TCCATGACAT GGACGCGGGC
    CACCTGAGTT TTGTGGAGGA GGTGTTTGAG AACCAGACCC GGCTCCCTGG AGGCCAGTGG
    ATCTACATGA GTGACAACTA CACAGATGTG AATGGGGATA AGGTGCTTCC TAAGGATGAC
    ATTGAGTGCC CACTGGGCTG GAAGTGGGAA GATGAAGAGT GGTCCACGGA CCTCAATCGG
    GCCGTCGATG AGCAAGGCTG GGAGTACAGT ATCACCATCC CCCCGGATCG GAAGCCAAAG
    CACTGGGTTC CTGCTGAGAA GATGTATTAC ACGCACCGTC GGCGGCGCTG GGTCCGCCTG
    CGCAGAAGGG ACCTCAGCCA GATGGAGGCA TTGAAGAGGC ACAGACAGGC AGAGGCAGAG
    GGTGAGGGCT GGGAGTACGC CTCGCTCTTG GGCTGGAAGT TCCACCTGGA GTACCGCAAG
    ACTGATGCTT TCCGCCGCCG CCGCTGGCGC CGCCGCATGG AACCGCTGGA GAAGACGGGG
    CCTGCAGCCG TGTTTGCCCT TGAGGGGGCT CTGGGTGGTG TGGTAGATGA CAGGAGTGAA
    GATTCCGTGT CCGTGTCCAC CTTGAGCTTT GGAGTGAACA GACCCACGAT TTCCTGCATC
    TTTGATTATG GGAACCGCTA CCACCTACGC TGCTACATGT ACCAGGCTCG GGACCTGCCC
    TCGATGGACA AGGACTCTTT TTCTGATCCC TACGCCATCG TCTCCTTCCT GCACCAGAGC
    CAGAAGACAG TGGTGGCGAA GAACACTCTG AACCCCACCT GGGACCAGAC GCTTATCTTC
    TATGAGATTG AGATCTTTGG TGAGCCAGCC AGTATCGCTG AGCAGCCGCC CAGCATTGTG
    GTGGAGCTGT ATGACCACGA CACCTACGGT GCAGATGAGT TTATGGGACG CTGCATTTGT
    CAGCCCAGCC TGGAGCGGAC ACCCCGGCTG GCCTGGTTCC CACTGATTAG GGGCAGCCAG
    TCGGCGGGGG AACTGCTGGC CTCTTTTGAG CTCATCCAGA GAGAAAAGCC GGCCATTCAC
    CATATTCCTG GTTTTGAGGT ACAGGATATG TCAGGAATCC TGGAAGAGCT GTGTGCACCT
    GCCTTCTTCC TCCAGGGCCT CCTCCAAACG TCAGAGGACA TAGACCTGCC CTACCCGCCA
    CCTCAGAGGG AGGCCAACAT CTTCATGGTC CCCCAGAACA TCAAGCCTGT TCTCCAGCGT
    ACTGCCATAG AGATCCTGGC GTGGGGCCTG CGGAACATGA AGAGCTACCA GCTAGCCAGC
    ATCTCCTCCC CTAGCCTTGT GGTAGAGTGC GGGGGCCAGA CAGTGCAGTC CTGTGTCATC
    AAGAACCTCC GGAAGAACCC CAACTTCGAC ATCTGTACCC TTTTCATGGA AGTGATGCTG
    CCCAAGGAGG AGGTCTACTG TCCCTCCATC GTGATCAAGG TCATTGATAA CCGCCAGTTT
    GGCCGCCGGC CTGTGGTGGG CCAGTGTACC ATCCGTTCCC TGGAGAGCTT CATGTGTGAC
    CTGTACTCCG AGGAGAATCC GTCCCCACAG GGCGGCCCAG ATGATGTGAG TTTACTCAGT
    CCTGGGGAAG ACGTGCTCAT TGACATCGAT GACAAGGAGC CCCTCATCCC CATGCAGGAG
    GAAGAGTTCA TCGACTGGTG GAGCAAATTC TTTGCCTCCA CAGGGGAGAG GGAAAAGTGC
    GGCTCATACT TGGAGAAGGG CTTTGACACT CTAAAGGTCT ATGACACACA ACTGGAGAAC
    GTGGAGGCCT TTGAGGGCCT GTCTGACTTT TGTAACACCT TCAAGCTCTA CCGGGGCAAG
    ACTCAGGAGG AGACAGAGGA CCCATCTGTT ATTGGGGAGT TTAAGGGCCT CTTCAAAATC
    TATCCCCTCC CAGAAGACCC GGCTGTTCCC ATGCCCCCGA GACAATTCCA CCAGCTGGCT
    GCCCAAGGGC CTCAGGAGTG CCTGGTCCGC ATCTATATCA TCCGAGCATT TGGCCTTCAG
    CCCAAGGACC CCAATGGGAA GTGTGATCCT TACATCAAGA TCTCCATAGG GAAGAAATCA
    GTGAGTGACC AGGATAACTA CATTCCCTGT ACTCTGGAGC CTGTGTTTGG AAAGATGTTC
    GAGCTGACCT GCACTCTGCC TCTGGAGAAG GACCTGAAGA TCACACTCTA TGACTATGAC
    CTCCTCTCCA AAGATGAAAA GATTGGGGAG ACAGTCATTG ACCTGGAGGA TAGGCTGCTG
    TCCAAGTTTG GGGCTCGCTG CGGACTCCCA CAGACGTACT GTGTCTCTGG ACCCAACCAG
    TGGAGGGACC AGCTCCGCCC CTCCCAGCTT CTCCACCTCT TCTGCCAGCA GCGCAGACTC
    AAGGCTCCTG TGTACCTCAC GGACCACGTG GTGTTCCAGG ATAAAGAATA CAGCATTGAA
    GAAATAGAGG CTGGCCGAGT CCCGAACCCA CACCTGGGCC CAGTGGAAGA GCGTCTGGCC
    TTGCATGTCC TCCAGCAGCA GGGCCTGGTC CCTGAGCATG TGGAGTCCCG GCCTCTCTAC
    AGCCCCCTGC AGCCAGACAT TGAACAGGGG AAGCTGCAGA TGTGGATCGA CCTATTTCCC
    AAGGCCCTGG GGCGTCCCGG ACCTCCCTTC AACATCACCC CGCGGAGAGC CAGAAGGTTT
    TTCCTGCGTT GTATCATCTG GAACACAAAG GACGTGATCC TGGACGACCT CAGCATCACA
    GGGGAGAAGA TGAGTGATAT TTATGTGAAG GGTTGGATGG TTGGCTTTGA AGAGCACAAG
    CAAAAGACAG ATGTGCATTA CCGGTCCCTG GGAGGTGAAG GCAACTTTAA CTGGAGGTTC
    ATTTTCCCCT TTGACTACCT GCCAGCCGAG CAAGTCTGCA CCGTCTCTAA GAAGGATGCT
    TTCTGGAGGC TGGACAAGAC TGAGAGCAAA ATCCCAGCAC GAGTGGTATT TCAGATCTGG
    GACAATGACA AGTTCTCCTT TGACGACTTT CTGGGCTCCT TGCAGTTGGA TCTCAACCAC
    ATGCCCAAGC CTGCCAAGAC AGCTGAGAAG TGCTCCTTGG ACCAGCTGGA TGACACTTTC
    CACCCGGAGT GGTTTGTGTC CCTTTTTGAA CAGAAAACAG TTAAGGGCTG GTGGCCCTGC
    GTAGCAGAAG AGGGCGAGAA GAAGATCCTG GCAGGCAAGC TGGAAATGAC CTTGGAGATC
    GTAGCAGAGA GTGAGCATGA GGAGCGGCCT GCTGGCCAGG GCCGGGATGA ACCCAACATG
    AACCCCAAGC TCGAGGACCC AAGGCGCCCT GACACCTCCT TCCTCTGGTT CACCTCTCCA
    TACAAGACTA TGAAGTTCAT CCTGTGGCGG CGGTTCCGAT GTGCCATTAT CCTCTTCATC
    ATTCTCTTCA TCCTGCTACT ATTCTTAGGC ATCTTCATCT ATGCTTTCCC GAACTACGCT
    GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe294461
    Clone ID Related Accession (Same CDS sequence) XM_022404852.1
    Accession Version XM_022404852.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6372bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product dysferlin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG ACACAGGAGG AGAGGAGGAC
    ACTGAGGACC AGGGGCTCAC TGGAGACGAG GGAGAGCCAT TACTGGATCA GAGTGGCGTC
    CCAGGCCCTG GGGCTCCCAC CACCCCGAGG AAGCCACCTT CCCATCTGCC CCCCTACCAC
    CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC GCAGCCAGAA AGCTGCTGTC CGACAAACCG
    CAGGACTTCC AGATCAGAGT GCAAGTGATC GAGGGCCGCC AGCTGCCAGG GGTGAATATC
    AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG CAGACCAAGA GGACACGGAT CCACAAGGGA
    AACAGCCCAC TATTTAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TTCGGAGCTG
    TTTGATGAGC CCATCTTTAT CACGGTCGTA GACTCCCGTT CTCTCAGGAC AGATGCTCTC
    ATTGGGGAAT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA TGCTTATCTG
    AGGAAGTGGC TGCTACTCTC CAACCCTGAT GACTTCTCTG TTGGGGCCAG AGGTTACCTG
    AAAGCAAGCC TTTATGTGCT GGGGCCCGGA GATGAAGCGC CTCTGGAGAG AAAGGACCCC
    TCTGAAGACA AGGAGGACAT TGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTGCGA
    GGAGCCCACT TCTGCCTGAA GATCTTCAGA GCGGAGGACT TACCACAAAT GGATGATGCC
    GTGATGGACA ATGTGAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA TTTGGTAGAT
    CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA
    GCCAACCCTC AGTGGAACCA GAGCATCACT CTGCCTGCCA TGTTTCCCTC CATGTGTGAA
    AAAATGAAGA TTCGCGTCAT GGACTGGGAC CGCCTTACCC ACAATGACAT CGTGGCCACC
    ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGAAGA GGAGCCTGCA
    GGTGTGGTCA AGCCTTCTCC AGCCACGGAC CTGGATGACC ATCTGGGCTT CCTTCCCACC
    TTCGGGCCCT GCTTCGTCAA TCTCTATGGC AGCCCCAGGG AATTCACTGG CTTCCCGGAC
    CCCTACGCGG AACTCAACAT GGGCAAGGGG GAAGGCGTGG CCTACCGAGG TCGGCTTCTG
    CTCTCCCTGG AGACCAAGCT GGTGGAGCAC AGCGGCGAGC AAAGGGTGGA GGACCTCCCT
    GCTGATGACA TCCTTCGGGT GGAGAAATAC CTCCGGAGGC GCAAGTACTC TCTCTTTGCC
    GCCTTCTACT CGGCCACCAT GCTCCAGGAT GTCGACGATG CCATCCAGTT TGAGGTCAGC
    ATTGGGAACT ATGGCAACAA GTTCGACACG ACCTGTCTGC CACTGGCCTC CACCACCCAG
    TACAGCCGGG CAGTCTTTGA TGGGTGCCAC TACTACTACT TGCCCTGGGG CAATGTGAAG
    CCTGTGGTGG TGCTGTCATC CTACTGGGAA GACATCAGCC ACAGAGTCGA GGCTCAGAAC
    CAGCTGCTCA GGATCGCTGA CCATCTGGAA GCTGGCCTGG AGCAAGTCCA CTTGGCTTTA
    AAGGCGCAGT TCAACACAGA GGATGTGGAC TCCCTGTTGG CCCAGCTGAT GGATGAGCTC
    ATCTCAGACT GCAGCCAGCC CCTGGGCAAT GTCCAAGAGA TACCCTCTGC CACCCACCTG
    GACCGGCACC TGTACAGGCT GCGCACCCGT CACCTAGGCC AGATTACTGA GGCGGCCCTG
    GCTCTGAAAC TCGGGCATGG CAAGCTCCCT GCCGCCCTGG AGCAGGCTGA AGACTGGCTC
    CTGCGTCTCC GTGCCCTGGC TGATGAGCCC CAGAACAGCC TGCCCGACAT CATCATCTGG
    ATGCTGCAGG GAAACAAGCG AGTGGCGTAC CAGCGGGTGC CTGCGCACGA GGTCCTCTTC
    TCCCGGCGGG GCGCCAGCTA CTGTGGCAAG AACTGTGGGA AGCTGCAGAC GATATTTCTG
    AAATATCCCA TGGAGGGGGT GCCCGGAGCC CGGATGCCAG TACAAATACG GGTGAAACTC
    TGGTTTGGGC TTTCTGTGGA TGAGAAGGAG TTCAACCAGT TTGCTGAGGG AAAGCTGTCC
    GTCTTTGCTG AAACTTATGA GAACCAGACC AAACTGGCCC TGGTTGGGAA CTGGGGCACA
    ACAGGCCTCA CCTACCCCAA GTTTTCTGAT GTCATGGGCA AGATCAAGCT ACCCAAGGAT
    AGCTTCCGCC CCTCAGCTGG CTGGACCTGG GCCGGAGACT GGTTCGTGTG TCCAGAGAAG
    ACCCTACTCC ATGACATGGA CGCGGGCCAC CTGAGTTTTG TGGAGGAGGT GTTTGAGAAC
    CAGACCCGGC TCCCTGGAGG CCAGTGGATC TACATGAGTG ACAACTACAC AGATGTGAAT
    GGGGATAAGG TGCTTCCTAA GGATGACATT GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT
    GAAGAGTGGT CCACGGACCT CAATCGGGCC GTCGATGAGC AAGGCTGGGA GTACAGTATC
    ACCATCCCCC CGGATCGGAA GCCAAAGCAC TGGGTTCCTG CTGAGAAGAT GTATTACACG
    CACCGTCGGC GGCGCTGGGT CCGCCTGCGC AGAAGGGACC TCAGCCAGAT GGAGGCATTG
    AAGAGGCACA GACAGGCAGA GGCAGAGGGT GAGGGCTGGG AGTACGCCTC GCTCTTGGGC
    TGGAAGTTCC ACCTGGAGTA CCGCAAGACT GATGCTTTCC GCCGCCGCCG CTGGCGCCGC
    CGCATGGAAC CGCTGGAGAA GACGGGGCCT GCAGCCGTGT TTGCCCTTGA GGGGGCTCTG
    GGTGGTGTGG TAGATGACAG GAGTGAAGAT TCCGTGTCCG TGTCCACCTT GAGCTTTGGA
    GTGAACAGAC CCACGATTTC CTGCATCTTT GATTATGGGA ACCGCTACCA CCTACGCTGC
    TACATGTACC AGGCTCGGGA CCTGCCCTCG ATGGACAAGG ACTCTTTTTC TGATCCCTAC
    GCCATCGTCT CCTTCCTGCA CCAGAGCCAG AAGACAGTGG TGGCGAAGAA CACTCTGAAC
    CCCACCTGGG ACCAGACGCT TATCTTCTAT GAGATTGAGA TCTTTGGTGA GCCAGCCAGT
    ATCGCTGAGC AGCCGCCCAG CATTGTGGTG GAGCTGTATG ACCACGACAC CTACGGTGCA
    GATGAGTTTA TGGGACGCTG CATTTGTCAG CCCAGCCTGG AGCGGACACC CCGGCTGGCC
    TGGTTCCCAC TGATTAGGGG CAGCCAGTCG GCGGGGGAAC TGCTGGCCTC TTTTGAGCTC
    ATCCAGAGAG AAAAGCCGGC CATTCACCAT ATTCCTGGTT TTGAGGTACA GGATATGTCA
    GGAATCCTGG AAGAGCTGTG TGCACCTGCC TTCTTCCTCC AGGGCCTCCT CCAAACGTCA
    GAGGACATAG ACCTGCCCTA CCCGCCACCT CAGAGGGAGG CCAACATCTT CATGGTCCCC
    CAGAACATCA AGCCTGTTCT CCAGCGTACT GCCATAGAGA TCCTGGCGTG GGGCCTGCGG
    AACATGAAGA GCTACCAGCT AGCCAGCATC TCCTCCCCTA GCCTTGTGGT AGAGTGCGGG
    GGCCAGACAG TGCAGTCCTG TGTCATCAAG AACCTCCGGA AGAACCCCAA CTTCGACATC
    TGTACCCTTT TCATGGAAGT GATGCTGCCC AAGGAGGAGG TCTACTGTCC CTCCATCGTG
    ATCAAGGTCA TTGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC
    CGTTCCCTGG AGAGCTTCAT GTGTGACCTG TACTCCGAGG AGAATCCGTC CCCACAGGGC
    GGCCCAGATG ATGTGAGTTT ACTCAGTCCT GGGGAAGACG TGCTCATTGA CATCGATGAC
    AAGGAGCCCC TCATCCCCAT GCAGCTTGCA GATGGTCTGT CGGGCTCTGC CCCCACTAAC
    ATGGCATCTT CTCCATCCAG TCCTCATAAG GCCCTCCTGG AGGAAGAGTT CATCGACTGG
    TGGAGCAAAT TCTTTGCCTC CACAGGGGAG AGGGAAAAGT GCGGCTCATA CTTGGAGAAG
    GGCTTTGACA CTCTAAAGGT CTATGACACA CAACTGGAGA ACGTGGAGGC CTTTGAGGGC
    CTGTCTGACT TTTGTAACAC CTTCAAGCTC TACCGGGGCA AGACTCAGGA GGAGACAGAG
    GACCCATCTG TTATTGGGGA GTTTAAGGGC CTCTTCAAAA TCTATCCCCT CCCAGAAGAC
    CCGGCTGTTC CCATGCCCCC GAGACAATTC CACCAGCTGG CTGCCCAAGG GCCTCAGGAG
    TGCCTGGTCC GCATCTATAT CATCCGAGCA TTTGGCCTTC AGCCCAAGGA CCCCAATGGG
    AAGTGTGATC CTTACATCAA GATCTCCATA GGGAAGAAAT CAGTGAGTGA CCAGGATAAC
    TACATTCCCT GTACTCTGGA GCCTGTGTTT GGAAAGATGT TCGAGCTGAC CTGCACTCTG
    CCTCTGGAGA AGGACCTGAA GATCACACTC TATGACTATG ACCTCCTCTC CAAAGATGAA
    AAGATTGGGG AGACAGTCAT TGACCTGGAG GATAGGCTGC TGTCCAAGTT TGGGGCTCGC
    TGCGGACTCC CACAGACGTA CTGTGTCTCT GGACCCAACC AGTGGAGGGA CCAGCTCCGC
    CCCTCCCAGC TTCTCCACCT CTTCTGCCAG CAGCGCAGAC TCAAGGCTCC TGTGTACCTC
    ACGGACCACG TGGTGTTCCA GGATAAAGAA TACAGCATTG AAGAAATAGA GGCTGGCCGA
    GTCCCGAACC CACACCTGGG CCCAGTGGAA GAGCGTCTGG CCTTGCATGT CCTCCAGCAG
    CAGGGCCTGG TCCCTGAGCA TGTGGAGTCC CGGCCTCTCT ACAGCCCCCT GCAGCCAGAC
    ATTGAACAGG GGAAGCTGCA GATGTGGATC GACCTATTTC CCAAGGCCCT GGGGCGTCCC
    GGACCTCCCT TCAACATCAC CCCGCGGAGA GCCAGAAGGT TTTTCCTGCG TTGTATCATC
    TGGAACACAA AGGACGTGAT CCTGGACGAC CTCAGCATCA CAGGGGAGAA GATGAGTGAT
    ATTTATGTGA AGGGTTGGAT GGTTGGCTTT GAAGAGCACA AGCAAAAGAC AGATGTGCAT
    TACCGGTCCC TGGGAGGTGA AGGCAACTTT AACTGGAGGT TCATTTTCCC CTTTGACTAC
    CTGCCAGCCG AGCAAGTCTG CACCGTCTCT AAGAAGGATG CTTTCTGGAG GCTGGACAAG
    ACTGAGAGCA AAATCCCAGC ACGAGTGGTA TTTCAGATCT GGGACAATGA CAAGTTCTCC
    TTTGACGACT TTCTGGGCTC CTTGCAGTTG GATCTCAACC ACATGCCCAA GCCTGCCAAG
    ACAGCTGAGA AGTGCTCCTT GGACCAGCTG GATGACACTT TCCACCCGGA GTGGTTTGTG
    TCCCTTTTTG AACAGAAAAC AGTTAAGGGC TGGTGGCCCT GCGTAGCAGA AGAGGGCGAG
    AAGAAGATCC TGGCAGGCAA GCTGGAAATG ACCTTGGAGA TCGTAGCAGA GAGTGAGCAT
    GAGGAGCGGC CTGCTGGCCA GGGCCGGGAT GAACCCAACA TGAACCCCAA GCTCGAGGAC
    CCAAGGCGCC CTGACACCTC CTTCCTCTGG TTCACCTCTC CATACAAGAC TATGAAGTTC
    ATCCTGTGGC GGCGGTTCCG ATGTGCCATT ATCCTCTTCA TCATTCTCTT CATCCTGCTA
    CTATTCTTAG GCATCTTCAT CTATGCTTTC CCGAACTACG CTGCCATGAA GCTGGTGAAG
    CCCTTCAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022260560.1
    CDS23..6394
    Translation

    Target ORF information:

    RefSeq Version XM_022404852.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris dysferlin (DYSF), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022404852.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG ACACAGGAGG AGAGGAGGAC
    ACTGAGGACC AGGGGCTCAC TGGAGACGAG GGAGAGCCAT TACTGGATCA GAGTGGCGTC
    CCAGGCCCTG GGGCTCCCAC CACCCCGAGG AAGCCACCTT CCCATCTGCC CCCCTACCAC
    CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC GCAGCCAGAA AGCTGCTGTC CGACAAACCG
    CAGGACTTCC AGATCAGAGT GCAAGTGATC GAGGGCCGCC AGCTGCCAGG GGTGAATATC
    AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG CAGACCAAGA GGACACGGAT CCACAAGGGA
    AACAGCCCAC TATTTAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TTCGGAGCTG
    TTTGATGAGC CCATCTTTAT CACGGTCGTA GACTCCCGTT CTCTCAGGAC AGATGCTCTC
    ATTGGGGAAT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA TGCTTATCTG
    AGGAAGTGGC TGCTACTCTC CAACCCTGAT GACTTCTCTG TTGGGGCCAG AGGTTACCTG
    AAAGCAAGCC TTTATGTGCT GGGGCCCGGA GATGAAGCGC CTCTGGAGAG AAAGGACCCC
    TCTGAAGACA AGGAGGACAT TGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTGCGA
    GGAGCCCACT TCTGCCTGAA GATCTTCAGA GCGGAGGACT TACCACAAAT GGATGATGCC
    GTGATGGACA ATGTGAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA TTTGGTAGAT
    CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA
    GCCAACCCTC AGTGGAACCA GAGCATCACT CTGCCTGCCA TGTTTCCCTC CATGTGTGAA
    AAAATGAAGA TTCGCGTCAT GGACTGGGAC CGCCTTACCC ACAATGACAT CGTGGCCACC
    ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGAAGA GGAGCCTGCA
    GGTGTGGTCA AGCCTTCTCC AGCCACGGAC CTGGATGACC ATCTGGGCTT CCTTCCCACC
    TTCGGGCCCT GCTTCGTCAA TCTCTATGGC AGCCCCAGGG AATTCACTGG CTTCCCGGAC
    CCCTACGCGG AACTCAACAT GGGCAAGGGG GAAGGCGTGG CCTACCGAGG TCGGCTTCTG
    CTCTCCCTGG AGACCAAGCT GGTGGAGCAC AGCGGCGAGC AAAGGGTGGA GGACCTCCCT
    GCTGATGACA TCCTTCGGGT GGAGAAATAC CTCCGGAGGC GCAAGTACTC TCTCTTTGCC
    GCCTTCTACT CGGCCACCAT GCTCCAGGAT GTCGACGATG CCATCCAGTT TGAGGTCAGC
    ATTGGGAACT ATGGCAACAA GTTCGACACG ACCTGTCTGC CACTGGCCTC CACCACCCAG
    TACAGCCGGG CAGTCTTTGA TGGGTGCCAC TACTACTACT TGCCCTGGGG CAATGTGAAG
    CCTGTGGTGG TGCTGTCATC CTACTGGGAA GACATCAGCC ACAGAGTCGA GGCTCAGAAC
    CAGCTGCTCA GGATCGCTGA CCATCTGGAA GCTGGCCTGG AGCAAGTCCA CTTGGCTTTA
    AAGGCGCAGT TCAACACAGA GGATGTGGAC TCCCTGTTGG CCCAGCTGAT GGATGAGCTC
    ATCTCAGACT GCAGCCAGCC CCTGGGCAAT GTCCAAGAGA TACCCTCTGC CACCCACCTG
    GACCGGCACC TGTACAGGCT GCGCACCCGT CACCTAGGCC AGATTACTGA GGCGGCCCTG
    GCTCTGAAAC TCGGGCATGG CAAGCTCCCT GCCGCCCTGG AGCAGGCTGA AGACTGGCTC
    CTGCGTCTCC GTGCCCTGGC TGATGAGCCC CAGAACAGCC TGCCCGACAT CATCATCTGG
    ATGCTGCAGG GAAACAAGCG AGTGGCGTAC CAGCGGGTGC CTGCGCACGA GGTCCTCTTC
    TCCCGGCGGG GCGCCAGCTA CTGTGGCAAG AACTGTGGGA AGCTGCAGAC GATATTTCTG
    AAATATCCCA TGGAGGGGGT GCCCGGAGCC CGGATGCCAG TACAAATACG GGTGAAACTC
    TGGTTTGGGC TTTCTGTGGA TGAGAAGGAG TTCAACCAGT TTGCTGAGGG AAAGCTGTCC
    GTCTTTGCTG AAACTTATGA GAACCAGACC AAACTGGCCC TGGTTGGGAA CTGGGGCACA
    ACAGGCCTCA CCTACCCCAA GTTTTCTGAT GTCATGGGCA AGATCAAGCT ACCCAAGGAT
    AGCTTCCGCC CCTCAGCTGG CTGGACCTGG GCCGGAGACT GGTTCGTGTG TCCAGAGAAG
    ACCCTACTCC ATGACATGGA CGCGGGCCAC CTGAGTTTTG TGGAGGAGGT GTTTGAGAAC
    CAGACCCGGC TCCCTGGAGG CCAGTGGATC TACATGAGTG ACAACTACAC AGATGTGAAT
    GGGGATAAGG TGCTTCCTAA GGATGACATT GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT
    GAAGAGTGGT CCACGGACCT CAATCGGGCC GTCGATGAGC AAGGCTGGGA GTACAGTATC
    ACCATCCCCC CGGATCGGAA GCCAAAGCAC TGGGTTCCTG CTGAGAAGAT GTATTACACG
    CACCGTCGGC GGCGCTGGGT CCGCCTGCGC AGAAGGGACC TCAGCCAGAT GGAGGCATTG
    AAGAGGCACA GACAGGCAGA GGCAGAGGGT GAGGGCTGGG AGTACGCCTC GCTCTTGGGC
    TGGAAGTTCC ACCTGGAGTA CCGCAAGACT GATGCTTTCC GCCGCCGCCG CTGGCGCCGC
    CGCATGGAAC CGCTGGAGAA GACGGGGCCT GCAGCCGTGT TTGCCCTTGA GGGGGCTCTG
    GGTGGTGTGG TAGATGACAG GAGTGAAGAT TCCGTGTCCG TGTCCACCTT GAGCTTTGGA
    GTGAACAGAC CCACGATTTC CTGCATCTTT GATTATGGGA ACCGCTACCA CCTACGCTGC
    TACATGTACC AGGCTCGGGA CCTGCCCTCG ATGGACAAGG ACTCTTTTTC TGATCCCTAC
    GCCATCGTCT CCTTCCTGCA CCAGAGCCAG AAGACAGTGG TGGCGAAGAA CACTCTGAAC
    CCCACCTGGG ACCAGACGCT TATCTTCTAT GAGATTGAGA TCTTTGGTGA GCCAGCCAGT
    ATCGCTGAGC AGCCGCCCAG CATTGTGGTG GAGCTGTATG ACCACGACAC CTACGGTGCA
    GATGAGTTTA TGGGACGCTG CATTTGTCAG CCCAGCCTGG AGCGGACACC CCGGCTGGCC
    TGGTTCCCAC TGATTAGGGG CAGCCAGTCG GCGGGGGAAC TGCTGGCCTC TTTTGAGCTC
    ATCCAGAGAG AAAAGCCGGC CATTCACCAT ATTCCTGGTT TTGAGGTACA GGATATGTCA
    GGAATCCTGG AAGAGCTGTG TGCACCTGCC TTCTTCCTCC AGGGCCTCCT CCAAACGTCA
    GAGGACATAG ACCTGCCCTA CCCGCCACCT CAGAGGGAGG CCAACATCTT CATGGTCCCC
    CAGAACATCA AGCCTGTTCT CCAGCGTACT GCCATAGAGA TCCTGGCGTG GGGCCTGCGG
    AACATGAAGA GCTACCAGCT AGCCAGCATC TCCTCCCCTA GCCTTGTGGT AGAGTGCGGG
    GGCCAGACAG TGCAGTCCTG TGTCATCAAG AACCTCCGGA AGAACCCCAA CTTCGACATC
    TGTACCCTTT TCATGGAAGT GATGCTGCCC AAGGAGGAGG TCTACTGTCC CTCCATCGTG
    ATCAAGGTCA TTGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC
    CGTTCCCTGG AGAGCTTCAT GTGTGACCTG TACTCCGAGG AGAATCCGTC CCCACAGGGC
    GGCCCAGATG ATGTGAGTTT ACTCAGTCCT GGGGAAGACG TGCTCATTGA CATCGATGAC
    AAGGAGCCCC TCATCCCCAT GCAGCTTGCA GATGGTCTGT CGGGCTCTGC CCCCACTAAC
    ATGGCATCTT CTCCATCCAG TCCTCATAAG GCCCTCCTGG AGGAAGAGTT CATCGACTGG
    TGGAGCAAAT TCTTTGCCTC CACAGGGGAG AGGGAAAAGT GCGGCTCATA CTTGGAGAAG
    GGCTTTGACA CTCTAAAGGT CTATGACACA CAACTGGAGA ACGTGGAGGC CTTTGAGGGC
    CTGTCTGACT TTTGTAACAC CTTCAAGCTC TACCGGGGCA AGACTCAGGA GGAGACAGAG
    GACCCATCTG TTATTGGGGA GTTTAAGGGC CTCTTCAAAA TCTATCCCCT CCCAGAAGAC
    CCGGCTGTTC CCATGCCCCC GAGACAATTC CACCAGCTGG CTGCCCAAGG GCCTCAGGAG
    TGCCTGGTCC GCATCTATAT CATCCGAGCA TTTGGCCTTC AGCCCAAGGA CCCCAATGGG
    AAGTGTGATC CTTACATCAA GATCTCCATA GGGAAGAAAT CAGTGAGTGA CCAGGATAAC
    TACATTCCCT GTACTCTGGA GCCTGTGTTT GGAAAGATGT TCGAGCTGAC CTGCACTCTG
    CCTCTGGAGA AGGACCTGAA GATCACACTC TATGACTATG ACCTCCTCTC CAAAGATGAA
    AAGATTGGGG AGACAGTCAT TGACCTGGAG GATAGGCTGC TGTCCAAGTT TGGGGCTCGC
    TGCGGACTCC CACAGACGTA CTGTGTCTCT GGACCCAACC AGTGGAGGGA CCAGCTCCGC
    CCCTCCCAGC TTCTCCACCT CTTCTGCCAG CAGCGCAGAC TCAAGGCTCC TGTGTACCTC
    ACGGACCACG TGGTGTTCCA GGATAAAGAA TACAGCATTG AAGAAATAGA GGCTGGCCGA
    GTCCCGAACC CACACCTGGG CCCAGTGGAA GAGCGTCTGG CCTTGCATGT CCTCCAGCAG
    CAGGGCCTGG TCCCTGAGCA TGTGGAGTCC CGGCCTCTCT ACAGCCCCCT GCAGCCAGAC
    ATTGAACAGG GGAAGCTGCA GATGTGGATC GACCTATTTC CCAAGGCCCT GGGGCGTCCC
    GGACCTCCCT TCAACATCAC CCCGCGGAGA GCCAGAAGGT TTTTCCTGCG TTGTATCATC
    TGGAACACAA AGGACGTGAT CCTGGACGAC CTCAGCATCA CAGGGGAGAA GATGAGTGAT
    ATTTATGTGA AGGGTTGGAT GGTTGGCTTT GAAGAGCACA AGCAAAAGAC AGATGTGCAT
    TACCGGTCCC TGGGAGGTGA AGGCAACTTT AACTGGAGGT TCATTTTCCC CTTTGACTAC
    CTGCCAGCCG AGCAAGTCTG CACCGTCTCT AAGAAGGATG CTTTCTGGAG GCTGGACAAG
    ACTGAGAGCA AAATCCCAGC ACGAGTGGTA TTTCAGATCT GGGACAATGA CAAGTTCTCC
    TTTGACGACT TTCTGGGCTC CTTGCAGTTG GATCTCAACC ACATGCCCAA GCCTGCCAAG
    ACAGCTGAGA AGTGCTCCTT GGACCAGCTG GATGACACTT TCCACCCGGA GTGGTTTGTG
    TCCCTTTTTG AACAGAAAAC AGTTAAGGGC TGGTGGCCCT GCGTAGCAGA AGAGGGCGAG
    AAGAAGATCC TGGCAGGCAA GCTGGAAATG ACCTTGGAGA TCGTAGCAGA GAGTGAGCAT
    GAGGAGCGGC CTGCTGGCCA GGGCCGGGAT GAACCCAACA TGAACCCCAA GCTCGAGGAC
    CCAAGGCGCC CTGACACCTC CTTCCTCTGG TTCACCTCTC CATACAAGAC TATGAAGTTC
    ATCCTGTGGC GGCGGTTCCG ATGTGCCATT ATCCTCTTCA TCATTCTCTT CATCCTGCTA
    CTATTCTTAG GCATCTTCAT CTATGCTTTC CCGAACTACG CTGCCATGAA GCTGGTGAAG
    CCCTTCAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe294462
    Clone ID Related Accession (Same CDS sequence) XM_022404853.1
    Accession Version XM_022404853.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6369bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product dysferlin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC
    CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC
    TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA
    CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGACA CAGGAGGAGA GGAGGACACT
    GAGGACCAGG GGCTCACTGG AGACGAGGGA GAGCCATTAC TGGATCAGAG TGGCGTCCCA
    GGCCCTGGGG CTCCCACCAC CCCGAGGAAG CCACCTTCCC ATCTGCCCCC CTACCACCCT
    GGGAGCAAGA GGAAAAGAAG TGCTCCCGCA GCCAGAAAGC TGCTGTCCGA CAAACCGCAG
    GACTTCCAGA TCAGAGTGCA AGTGATCGAG GGCCGCCAGC TGCCAGGGGT GAATATCAAG
    CCTGTGATCA AGGTCACTGC TGCTGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC
    AGCCCACTAT TTAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTTC GGAGCTGTTT
    GATGAGCCCA TCTTTATCAC GGTCGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT
    GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACATGC TTATCTGAGG
    AAGTGGCTGC TACTCTCCAA CCCTGATGAC TTCTCTGTTG GGGCCAGAGG TTACCTGAAA
    GCAAGCCTTT ATGTGCTGGG GCCCGGAGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT
    GAAGACAAGG AGGACATTGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTGCGAGGA
    GCCCACTTCT GCCTGAAGAT CTTCAGAGCG GAGGACTTAC CACAAATGGA TGATGCCGTG
    ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAATTT GGTAGATCCC
    TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC
    AACCCTCAGT GGAACCAGAG CATCACTCTG CCTGCCATGT TTCCCTCCAT GTGTGAAAAA
    ATGAAGATTC GCGTCATGGA CTGGGACCGC CTTACCCACA ATGACATCGT GGCCACCACC
    TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT
    GTGGTCAAGC CTTCTCCAGC CACGGACCTG GATGACCATC TGGGCTTCCT TCCCACCTTC
    GGGCCCTGCT TCGTCAATCT CTATGGCAGC CCCAGGGAAT TCACTGGCTT CCCGGACCCC
    TACGCGGAAC TCAACATGGG CAAGGGGGAA GGCGTGGCCT ACCGAGGTCG GCTTCTGCTC
    TCCCTGGAGA CCAAGCTGGT GGAGCACAGC GGCGAGCAAA GGGTGGAGGA CCTCCCTGCT
    GATGACATCC TTCGGGTGGA GAAATACCTC CGGAGGCGCA AGTACTCTCT CTTTGCCGCC
    TTCTACTCGG CCACCATGCT CCAGGATGTC GACGATGCCA TCCAGTTTGA GGTCAGCATT
    GGGAACTATG GCAACAAGTT CGACACGACC TGTCTGCCAC TGGCCTCCAC CACCCAGTAC
    AGCCGGGCAG TCTTTGATGG GTGCCACTAC TACTACTTGC CCTGGGGCAA TGTGAAGCCT
    GTGGTGGTGC TGTCATCCTA CTGGGAAGAC ATCAGCCACA GAGTCGAGGC TCAGAACCAG
    CTGCTCAGGA TCGCTGACCA TCTGGAAGCT GGCCTGGAGC AAGTCCACTT GGCTTTAAAG
    GCGCAGTTCA ACACAGAGGA TGTGGACTCC CTGTTGGCCC AGCTGATGGA TGAGCTCATC
    TCAGACTGCA GCCAGCCCCT GGGCAATGTC CAAGAGATAC CCTCTGCCAC CCACCTGGAC
    CGGCACCTGT ACAGGCTGCG CACCCGTCAC CTAGGCCAGA TTACTGAGGC GGCCCTGGCT
    CTGAAACTCG GGCATGGCAA GCTCCCTGCC GCCCTGGAGC AGGCTGAAGA CTGGCTCCTG
    CGTCTCCGTG CCCTGGCTGA TGAGCCCCAG AACAGCCTGC CCGACATCAT CATCTGGATG
    CTGCAGGGAA ACAAGCGAGT GGCGTACCAG CGGGTGCCTG CGCACGAGGT CCTCTTCTCC
    CGGCGGGGCG CCAGCTACTG TGGCAAGAAC TGTGGGAAGC TGCAGACGAT ATTTCTGAAA
    TATCCCATGG AGGGGGTGCC CGGAGCCCGG ATGCCAGTAC AAATACGGGT GAAACTCTGG
    TTTGGGCTTT CTGTGGATGA GAAGGAGTTC AACCAGTTTG CTGAGGGAAA GCTGTCCGTC
    TTTGCTGAAA CTTATGAGAA CCAGACCAAA CTGGCCCTGG TTGGGAACTG GGGCACAACA
    GGCCTCACCT ACCCCAAGTT TTCTGATGTC ATGGGCAAGA TCAAGCTACC CAAGGATAGC
    TTCCGCCCCT CAGCTGGCTG GACCTGGGCC GGAGACTGGT TCGTGTGTCC AGAGAAGACC
    CTACTCCATG ACATGGACGC GGGCCACCTG AGTTTTGTGG AGGAGGTGTT TGAGAACCAG
    ACCCGGCTCC CTGGAGGCCA GTGGATCTAC ATGAGTGACA ACTACACAGA TGTGAATGGG
    GATAAGGTGC TTCCTAAGGA TGACATTGAG TGCCCACTGG GCTGGAAGTG GGAAGATGAA
    GAGTGGTCCA CGGACCTCAA TCGGGCCGTC GATGAGCAAG GCTGGGAGTA CAGTATCACC
    ATCCCCCCGG ATCGGAAGCC AAAGCACTGG GTTCCTGCTG AGAAGATGTA TTACACGCAC
    CGTCGGCGGC GCTGGGTCCG CCTGCGCAGA AGGGACCTCA GCCAGATGGA GGCATTGAAG
    AGGCACAGAC AGGCAGAGGC AGAGGGTGAG GGCTGGGAGT ACGCCTCGCT CTTGGGCTGG
    AAGTTCCACC TGGAGTACCG CAAGACTGAT GCTTTCCGCC GCCGCCGCTG GCGCCGCCGC
    ATGGAACCGC TGGAGAAGAC GGGGCCTGCA GCCGTGTTTG CCCTTGAGGG GGCTCTGGGT
    GGTGTGGTAG ATGACAGGAG TGAAGATTCC GTGTCCGTGT CCACCTTGAG CTTTGGAGTG
    AACAGACCCA CGATTTCCTG CATCTTTGAT TATGGGAACC GCTACCACCT ACGCTGCTAC
    ATGTACCAGG CTCGGGACCT GCCCTCGATG GACAAGGACT CTTTTTCTGA TCCCTACGCC
    ATCGTCTCCT TCCTGCACCA GAGCCAGAAG ACAGTGGTGG CGAAGAACAC TCTGAACCCC
    ACCTGGGACC AGACGCTTAT CTTCTATGAG ATTGAGATCT TTGGTGAGCC AGCCAGTATC
    GCTGAGCAGC CGCCCAGCAT TGTGGTGGAG CTGTATGACC ACGACACCTA CGGTGCAGAT
    GAGTTTATGG GACGCTGCAT TTGTCAGCCC AGCCTGGAGC GGACACCCCG GCTGGCCTGG
    TTCCCACTGA TTAGGGGCAG CCAGTCGGCG GGGGAACTGC TGGCCTCTTT TGAGCTCATC
    CAGAGAGAAA AGCCGGCCAT TCACCATATT CCTGGTTTTG AGGTACAGGA TATGTCAGGA
    ATCCTGGAAG AGCTGTGTGC ACCTGCCTTC TTCCTCCAGG GCCTCCTCCA AACGTCAGAG
    GACATAGACC TGCCCTACCC GCCACCTCAG AGGGAGGCCA ACATCTTCAT GGTCCCCCAG
    AACATCAAGC CTGTTCTCCA GCGTACTGCC ATAGAGATCC TGGCGTGGGG CCTGCGGAAC
    ATGAAGAGCT ACCAGCTAGC CAGCATCTCC TCCCCTAGCC TTGTGGTAGA GTGCGGGGGC
    CAGACAGTGC AGTCCTGTGT CATCAAGAAC CTCCGGAAGA ACCCCAACTT CGACATCTGT
    ACCCTTTTCA TGGAAGTGAT GCTGCCCAAG GAGGAGGTCT ACTGTCCCTC CATCGTGATC
    AAGGTCATTG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGT
    TCCCTGGAGA GCTTCATGTG TGACCTGTAC TCCGAGGAGA ATCCGTCCCC ACAGGGCGGC
    CCAGATGATG TGAGTTTACT CAGTCCTGGG GAAGACGTGC TCATTGACAT CGATGACAAG
    GAGCCCCTCA TCCCCATGCA GCTTGCAGAT GGTCTGTCGG GCTCTGCCCC CACTAACATG
    GCATCTTCTC CATCCAGTCC TCATAAGGCC CTCCTGGAGG AAGAGTTCAT CGACTGGTGG
    AGCAAATTCT TTGCCTCCAC AGGGGAGAGG GAAAAGTGCG GCTCATACTT GGAGAAGGGC
    TTTGACACTC TAAAGGTCTA TGACACACAA CTGGAGAACG TGGAGGCCTT TGAGGGCCTG
    TCTGACTTTT GTAACACCTT CAAGCTCTAC CGGGGCAAGA CTCAGGAGGA GACAGAGGAC
    CCATCTGTTA TTGGGGAGTT TAAGGGCCTC TTCAAAATCT ATCCCCTCCC AGAAGACCCG
    GCTGTTCCCA TGCCCCCGAG ACAATTCCAC CAGCTGGCTG CCCAAGGGCC TCAGGAGTGC
    CTGGTCCGCA TCTATATCAT CCGAGCATTT GGCCTTCAGC CCAAGGACCC CAATGGGAAG
    TGTGATCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC
    ATTCCCTGTA CTCTGGAGCC TGTGTTTGGA AAGATGTTCG AGCTGACCTG CACTCTGCCT
    CTGGAGAAGG ACCTGAAGAT CACACTCTAT GACTATGACC TCCTCTCCAA AGATGAAAAG
    ATTGGGGAGA CAGTCATTGA CCTGGAGGAT AGGCTGCTGT CCAAGTTTGG GGCTCGCTGC
    GGACTCCCAC AGACGTACTG TGTCTCTGGA CCCAACCAGT GGAGGGACCA GCTCCGCCCC
    TCCCAGCTTC TCCACCTCTT CTGCCAGCAG CGCAGACTCA AGGCTCCTGT GTACCTCACG
    GACCACGTGG TGTTCCAGGA TAAAGAATAC AGCATTGAAG AAATAGAGGC TGGCCGAGTC
    CCGAACCCAC ACCTGGGCCC AGTGGAAGAG CGTCTGGCCT TGCATGTCCT CCAGCAGCAG
    GGCCTGGTCC CTGAGCATGT GGAGTCCCGG CCTCTCTACA GCCCCCTGCA GCCAGACATT
    GAACAGGGGA AGCTGCAGAT GTGGATCGAC CTATTTCCCA AGGCCCTGGG GCGTCCCGGA
    CCTCCCTTCA ACATCACCCC GCGGAGAGCC AGAAGGTTTT TCCTGCGTTG TATCATCTGG
    AACACAAAGG ACGTGATCCT GGACGACCTC AGCATCACAG GGGAGAAGAT GAGTGATATT
    TATGTGAAGG GTTGGATGGT TGGCTTTGAA GAGCACAAGC AAAAGACAGA TGTGCATTAC
    CGGTCCCTGG GAGGTGAAGG CAACTTTAAC TGGAGGTTCA TTTTCCCCTT TGACTACCTG
    CCAGCCGAGC AAGTCTGCAC CGTCTCTAAG AAGGATGCTT TCTGGAGGCT GGACAAGACT
    GAGAGCAAAA TCCCAGCACG AGTGGTATTT CAGATCTGGG ACAATGACAA GTTCTCCTTT
    GACGACTTTC TGGGCTCCTT GCAGTTGGAT CTCAACCACA TGCCCAAGCC TGCCAAGACA
    GCTGAGAAGT GCTCCTTGGA CCAGCTGGAT GACACTTTCC ACCCGGAGTG GTTTGTGTCC
    CTTTTTGAAC AGAAAACAGT TAAGGGCTGG TGGCCCTGCG TAGCAGAAGA GGGCGAGAAG
    AAGATCCTGG CAGGCAAGCT GGAAATGACC TTGGAGATCG TAGCAGAGAG TGAGCATGAG
    GAGCGGCCTG CTGGCCAGGG CCGGGATGAA CCCAACATGA ACCCCAAGCT CGAGGACCCA
    AGGCGCCCTG ACACCTCCTT CCTCTGGTTC ACCTCTCCAT ACAAGACTAT GAAGTTCATC
    CTGTGGCGGC GGTTCCGATG TGCCATTATC CTCTTCATCA TTCTCTTCAT CCTGCTACTA
    TTCTTAGGCA TCTTCATCTA TGCTTTCCCG AACTACGCTG CCATGAAGCT GGTGAAGCCC
    TTCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022260561.1
    CDS406..6774
    Translation

    Target ORF information:

    RefSeq Version XM_022404853.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris dysferlin (DYSF), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022404853.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC
    CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC
    TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA
    CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGACA CAGGAGGAGA GGAGGACACT
    GAGGACCAGG GGCTCACTGG AGACGAGGGA GAGCCATTAC TGGATCAGAG TGGCGTCCCA
    GGCCCTGGGG CTCCCACCAC CCCGAGGAAG CCACCTTCCC ATCTGCCCCC CTACCACCCT
    GGGAGCAAGA GGAAAAGAAG TGCTCCCGCA GCCAGAAAGC TGCTGTCCGA CAAACCGCAG
    GACTTCCAGA TCAGAGTGCA AGTGATCGAG GGCCGCCAGC TGCCAGGGGT GAATATCAAG
    CCTGTGATCA AGGTCACTGC TGCTGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC
    AGCCCACTAT TTAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTTC GGAGCTGTTT
    GATGAGCCCA TCTTTATCAC GGTCGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT
    GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACATGC TTATCTGAGG
    AAGTGGCTGC TACTCTCCAA CCCTGATGAC TTCTCTGTTG GGGCCAGAGG TTACCTGAAA
    GCAAGCCTTT ATGTGCTGGG GCCCGGAGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT
    GAAGACAAGG AGGACATTGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTGCGAGGA
    GCCCACTTCT GCCTGAAGAT CTTCAGAGCG GAGGACTTAC CACAAATGGA TGATGCCGTG
    ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAATTT GGTAGATCCC
    TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC
    AACCCTCAGT GGAACCAGAG CATCACTCTG CCTGCCATGT TTCCCTCCAT GTGTGAAAAA
    ATGAAGATTC GCGTCATGGA CTGGGACCGC CTTACCCACA ATGACATCGT GGCCACCACC
    TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT
    GTGGTCAAGC CTTCTCCAGC CACGGACCTG GATGACCATC TGGGCTTCCT TCCCACCTTC
    GGGCCCTGCT TCGTCAATCT CTATGGCAGC CCCAGGGAAT TCACTGGCTT CCCGGACCCC
    TACGCGGAAC TCAACATGGG CAAGGGGGAA GGCGTGGCCT ACCGAGGTCG GCTTCTGCTC
    TCCCTGGAGA CCAAGCTGGT GGAGCACAGC GGCGAGCAAA GGGTGGAGGA CCTCCCTGCT
    GATGACATCC TTCGGGTGGA GAAATACCTC CGGAGGCGCA AGTACTCTCT CTTTGCCGCC
    TTCTACTCGG CCACCATGCT CCAGGATGTC GACGATGCCA TCCAGTTTGA GGTCAGCATT
    GGGAACTATG GCAACAAGTT CGACACGACC TGTCTGCCAC TGGCCTCCAC CACCCAGTAC
    AGCCGGGCAG TCTTTGATGG GTGCCACTAC TACTACTTGC CCTGGGGCAA TGTGAAGCCT
    GTGGTGGTGC TGTCATCCTA CTGGGAAGAC ATCAGCCACA GAGTCGAGGC TCAGAACCAG
    CTGCTCAGGA TCGCTGACCA TCTGGAAGCT GGCCTGGAGC AAGTCCACTT GGCTTTAAAG
    GCGCAGTTCA ACACAGAGGA TGTGGACTCC CTGTTGGCCC AGCTGATGGA TGAGCTCATC
    TCAGACTGCA GCCAGCCCCT GGGCAATGTC CAAGAGATAC CCTCTGCCAC CCACCTGGAC
    CGGCACCTGT ACAGGCTGCG CACCCGTCAC CTAGGCCAGA TTACTGAGGC GGCCCTGGCT
    CTGAAACTCG GGCATGGCAA GCTCCCTGCC GCCCTGGAGC AGGCTGAAGA CTGGCTCCTG
    CGTCTCCGTG CCCTGGCTGA TGAGCCCCAG AACAGCCTGC CCGACATCAT CATCTGGATG
    CTGCAGGGAA ACAAGCGAGT GGCGTACCAG CGGGTGCCTG CGCACGAGGT CCTCTTCTCC
    CGGCGGGGCG CCAGCTACTG TGGCAAGAAC TGTGGGAAGC TGCAGACGAT ATTTCTGAAA
    TATCCCATGG AGGGGGTGCC CGGAGCCCGG ATGCCAGTAC AAATACGGGT GAAACTCTGG
    TTTGGGCTTT CTGTGGATGA GAAGGAGTTC AACCAGTTTG CTGAGGGAAA GCTGTCCGTC
    TTTGCTGAAA CTTATGAGAA CCAGACCAAA CTGGCCCTGG TTGGGAACTG GGGCACAACA
    GGCCTCACCT ACCCCAAGTT TTCTGATGTC ATGGGCAAGA TCAAGCTACC CAAGGATAGC
    TTCCGCCCCT CAGCTGGCTG GACCTGGGCC GGAGACTGGT TCGTGTGTCC AGAGAAGACC
    CTACTCCATG ACATGGACGC GGGCCACCTG AGTTTTGTGG AGGAGGTGTT TGAGAACCAG
    ACCCGGCTCC CTGGAGGCCA GTGGATCTAC ATGAGTGACA ACTACACAGA TGTGAATGGG
    GATAAGGTGC TTCCTAAGGA TGACATTGAG TGCCCACTGG GCTGGAAGTG GGAAGATGAA
    GAGTGGTCCA CGGACCTCAA TCGGGCCGTC GATGAGCAAG GCTGGGAGTA CAGTATCACC
    ATCCCCCCGG ATCGGAAGCC AAAGCACTGG GTTCCTGCTG AGAAGATGTA TTACACGCAC
    CGTCGGCGGC GCTGGGTCCG CCTGCGCAGA AGGGACCTCA GCCAGATGGA GGCATTGAAG
    AGGCACAGAC AGGCAGAGGC AGAGGGTGAG GGCTGGGAGT ACGCCTCGCT CTTGGGCTGG
    AAGTTCCACC TGGAGTACCG CAAGACTGAT GCTTTCCGCC GCCGCCGCTG GCGCCGCCGC
    ATGGAACCGC TGGAGAAGAC GGGGCCTGCA GCCGTGTTTG CCCTTGAGGG GGCTCTGGGT
    GGTGTGGTAG ATGACAGGAG TGAAGATTCC GTGTCCGTGT CCACCTTGAG CTTTGGAGTG
    AACAGACCCA CGATTTCCTG CATCTTTGAT TATGGGAACC GCTACCACCT ACGCTGCTAC
    ATGTACCAGG CTCGGGACCT GCCCTCGATG GACAAGGACT CTTTTTCTGA TCCCTACGCC
    ATCGTCTCCT TCCTGCACCA GAGCCAGAAG ACAGTGGTGG CGAAGAACAC TCTGAACCCC
    ACCTGGGACC AGACGCTTAT CTTCTATGAG ATTGAGATCT TTGGTGAGCC AGCCAGTATC
    GCTGAGCAGC CGCCCAGCAT TGTGGTGGAG CTGTATGACC ACGACACCTA CGGTGCAGAT
    GAGTTTATGG GACGCTGCAT TTGTCAGCCC AGCCTGGAGC GGACACCCCG GCTGGCCTGG
    TTCCCACTGA TTAGGGGCAG CCAGTCGGCG GGGGAACTGC TGGCCTCTTT TGAGCTCATC
    CAGAGAGAAA AGCCGGCCAT TCACCATATT CCTGGTTTTG AGGTACAGGA TATGTCAGGA
    ATCCTGGAAG AGCTGTGTGC ACCTGCCTTC TTCCTCCAGG GCCTCCTCCA AACGTCAGAG
    GACATAGACC TGCCCTACCC GCCACCTCAG AGGGAGGCCA ACATCTTCAT GGTCCCCCAG
    AACATCAAGC CTGTTCTCCA GCGTACTGCC ATAGAGATCC TGGCGTGGGG CCTGCGGAAC
    ATGAAGAGCT ACCAGCTAGC CAGCATCTCC TCCCCTAGCC TTGTGGTAGA GTGCGGGGGC
    CAGACAGTGC AGTCCTGTGT CATCAAGAAC CTCCGGAAGA ACCCCAACTT CGACATCTGT
    ACCCTTTTCA TGGAAGTGAT GCTGCCCAAG GAGGAGGTCT ACTGTCCCTC CATCGTGATC
    AAGGTCATTG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGT
    TCCCTGGAGA GCTTCATGTG TGACCTGTAC TCCGAGGAGA ATCCGTCCCC ACAGGGCGGC
    CCAGATGATG TGAGTTTACT CAGTCCTGGG GAAGACGTGC TCATTGACAT CGATGACAAG
    GAGCCCCTCA TCCCCATGCA GCTTGCAGAT GGTCTGTCGG GCTCTGCCCC CACTAACATG
    GCATCTTCTC CATCCAGTCC TCATAAGGCC CTCCTGGAGG AAGAGTTCAT CGACTGGTGG
    AGCAAATTCT TTGCCTCCAC AGGGGAGAGG GAAAAGTGCG GCTCATACTT GGAGAAGGGC
    TTTGACACTC TAAAGGTCTA TGACACACAA CTGGAGAACG TGGAGGCCTT TGAGGGCCTG
    TCTGACTTTT GTAACACCTT CAAGCTCTAC CGGGGCAAGA CTCAGGAGGA GACAGAGGAC
    CCATCTGTTA TTGGGGAGTT TAAGGGCCTC TTCAAAATCT ATCCCCTCCC AGAAGACCCG
    GCTGTTCCCA TGCCCCCGAG ACAATTCCAC CAGCTGGCTG CCCAAGGGCC TCAGGAGTGC
    CTGGTCCGCA TCTATATCAT CCGAGCATTT GGCCTTCAGC CCAAGGACCC CAATGGGAAG
    TGTGATCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC
    ATTCCCTGTA CTCTGGAGCC TGTGTTTGGA AAGATGTTCG AGCTGACCTG CACTCTGCCT
    CTGGAGAAGG ACCTGAAGAT CACACTCTAT GACTATGACC TCCTCTCCAA AGATGAAAAG
    ATTGGGGAGA CAGTCATTGA CCTGGAGGAT AGGCTGCTGT CCAAGTTTGG GGCTCGCTGC
    GGACTCCCAC AGACGTACTG TGTCTCTGGA CCCAACCAGT GGAGGGACCA GCTCCGCCCC
    TCCCAGCTTC TCCACCTCTT CTGCCAGCAG CGCAGACTCA AGGCTCCTGT GTACCTCACG
    GACCACGTGG TGTTCCAGGA TAAAGAATAC AGCATTGAAG AAATAGAGGC TGGCCGAGTC
    CCGAACCCAC ACCTGGGCCC AGTGGAAGAG CGTCTGGCCT TGCATGTCCT CCAGCAGCAG
    GGCCTGGTCC CTGAGCATGT GGAGTCCCGG CCTCTCTACA GCCCCCTGCA GCCAGACATT
    GAACAGGGGA AGCTGCAGAT GTGGATCGAC CTATTTCCCA AGGCCCTGGG GCGTCCCGGA
    CCTCCCTTCA ACATCACCCC GCGGAGAGCC AGAAGGTTTT TCCTGCGTTG TATCATCTGG
    AACACAAAGG ACGTGATCCT GGACGACCTC AGCATCACAG GGGAGAAGAT GAGTGATATT
    TATGTGAAGG GTTGGATGGT TGGCTTTGAA GAGCACAAGC AAAAGACAGA TGTGCATTAC
    CGGTCCCTGG GAGGTGAAGG CAACTTTAAC TGGAGGTTCA TTTTCCCCTT TGACTACCTG
    CCAGCCGAGC AAGTCTGCAC CGTCTCTAAG AAGGATGCTT TCTGGAGGCT GGACAAGACT
    GAGAGCAAAA TCCCAGCACG AGTGGTATTT CAGATCTGGG ACAATGACAA GTTCTCCTTT
    GACGACTTTC TGGGCTCCTT GCAGTTGGAT CTCAACCACA TGCCCAAGCC TGCCAAGACA
    GCTGAGAAGT GCTCCTTGGA CCAGCTGGAT GACACTTTCC ACCCGGAGTG GTTTGTGTCC
    CTTTTTGAAC AGAAAACAGT TAAGGGCTGG TGGCCCTGCG TAGCAGAAGA GGGCGAGAAG
    AAGATCCTGG CAGGCAAGCT GGAAATGACC TTGGAGATCG TAGCAGAGAG TGAGCATGAG
    GAGCGGCCTG CTGGCCAGGG CCGGGATGAA CCCAACATGA ACCCCAAGCT CGAGGACCCA
    AGGCGCCCTG ACACCTCCTT CCTCTGGTTC ACCTCTCCAT ACAAGACTAT GAAGTTCATC
    CTGTGGCGGC GGTTCCGATG TGCCATTATC CTCTTCATCA TTCTCTTCAT CCTGCTACTA
    TTCTTAGGCA TCTTCATCTA TGCTTTCCCG AACTACGCTG CCATGAAGCT GGTGAAGCCC
    TTCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe294463
    Clone ID Related Accession (Same CDS sequence) XM_022404854.1
    Accession Version XM_022404854.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6240bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product dysferlin isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGGGAGGA ACAGGTTCTT AGGGGAAGCC CAAGTCCCAC TCCAGGAGGT CCTCGCCACT 
    CCTAGTCTGT CTGCCAGCTT CAATGCCCCC CTCCTGGACG CCAAGAAGCA GCCCACGGGG
    GCCTCGCTGG TCCTGCAGGT GTCCTACACT CCACTCCCTG GAGCCGTGCC CCTGTTCCCA
    CCTGCTGCAT CTCTGGAACC CTCCCCGACT CTGCCTGACA TGGATATGGG GGCCGGTGGG
    GGACAGAGCC GGGCTGAGAC TTGGTCCCTG CTCAGTGACA GCACCGTGGA TACAAGATAC
    TCTGGAAAGA AGTGGCTGGC CCCCACGGAC ACAGGAGGAG AGGAGGACAC TGAGGACCAG
    GGGCTCACTG GAGACGAGGG AGAGCCATTA CTGGATCAGA GTGGCGTCCC AGGCCCTGGG
    GCTCCCACCA CCCCGAGGAA GCCACCTTCC CATCTGCCCC CCTACCACCC TGGGAGCAAG
    AGGAAAAGAA GTGCTCCCGC AGCCAGAAAG CTGCTGTCCG ACAAACCGCA GGACTTCCAG
    ATCAGAGTGC AAGTGATCGA GGGCCGCCAG CTGCCAGGGG TGAATATCAA GCCTGTGATC
    AAGGTCACTG CTGCTGGGCA GACCAAGAGG ACACGGATCC ACAAGGGAAA CAGCCCACTA
    TTTAACGAGA CTCTTTTCTT CAATGTGTTT GACTCTCCTT CGGAGCTGTT TGATGAGCCC
    ATCTTTATCA CGGTCGTAGA CTCCCGTTCT CTCAGGACAG ATGCTCTCAT TGGGGAATTC
    CGGATGGATG TGGGTACCAT CTACAGAGAG CCCCGACATG CTTATCTGAG GAAGTGGCTG
    CTACTCTCCA ACCCTGATGA CTTCTCTGTT GGGGCCAGAG GTTACCTGAA AGCAAGCCTT
    TATGTGCTGG GGCCCGGAGA TGAAGCGCCT CTGGAGAGAA AGGACCCCTC TGAAGACAAG
    GAGGACATTG AAAACAATCT GCTCAGGCCA ACTGGCGTGG CCCTGCGAGG AGCCCACTTC
    TGCCTGAAGA TCTTCAGAGC GGAGGACTTA CCACAAATGG ATGATGCCGT GATGGACAAT
    GTGAAGCAGA TCTTTGGCTT TGACAGCAAC AAGAAGAATT TGGTAGATCC CTTCATGGAA
    GTCAGCTTTG CGGGGAAAAT GCTCTGCAGC AAGATCCTGG AGAAGACAGC CAACCCTCAG
    TGGAACCAGA GCATCACTCT GCCTGCCATG TTTCCCTCCA TGTGTGAAAA AATGAAGATT
    CGCGTCATGG ACTGGGACCG CCTTACCCAC AATGACATCG TGGCCACCAC CTACCTGAGT
    ATGTCGAAAA TCTCTGCCCC TGGAGGAGAA ATAGAAGAGG AGCCTGCAGG TGTGGTCAAG
    CCTTCTCCAG CCACGGACCT GGATGACCAT CTGGGCTTCC TTCCCACCTT CGGGCCCTGC
    TTCGTCAATC TCTATGGCAG CCCCAGGGAA TTCACTGGCT TCCCGGACCC CTACGCGGAA
    CTCAACATGG GCAAGGGGGA AGGCGTGGCC TACCGAGGTC GGCTTCTGCT CTCCCTGGAG
    ACCAAGCTGG TGGAGCACAG CGGCGAGCAA AGGGTGGAGG ACCTCCCTGC TGATGACATC
    CTTCGGGTGG AGAAATACCT CCGGAGGCGC AAGTACTCTC TCTTTGCCGC CTTCTACTCG
    GCCACCATGC TCCAGGATGT CGACGATGCC ATCCAGTTTG AGGTCAGCAT TGGGAACTAT
    GGCAACAAGT TCGACACGAC CTGTCTGCCA CTGGCCTCCA CCACCCAGTA CAGCCGGGCA
    GTCTTTGATG GGTGCCACTA CTACTACTTG CCCTGGGGCA ATGTGAAGCC TGTGGTGGTG
    CTGTCATCCT ACTGGGAAGA CATCAGCCAC AGAGTCGAGG CTCAGAACCA GCTGCTCAGG
    ATCGCTGACC ATCTGGAAGC TGGCCTGGAG CAAGTCCACT TGGCTTTAAA GGCGCAGTTC
    AACACAGAGG ATGTGGACTC CCTGTTGGCC CAGCTGATGG ATGAGCTCAT CTCAGACTGC
    AGCCAGCCCC TGGGCAATGT CCAAGAGATA CCCTCTGCCA CCCACCTGGA CCGGCACCTG
    TACAGGCTGC GCACCCGTCA CCTAGGCCAG ATTACTGAGG CGGCCCTGGC TCTGAAACTC
    GGGCATGGCA AGCTCCCTGC CGCCCTGGAG CAGGCTGAAG ACTGGCTCCT GCGTCTCCGT
    GCCCTGGCTG ATGAGCCCCA GAACAGCCTG CCCGACATCA TCATCTGGAT GCTGCAGGGA
    AACAAGCGAG TGGCGTACCA GCGGGTGCCT GCGCACGAGG TCCTCTTCTC CCGGCGGGGC
    GCCAGCTACT GTGGCAAGAA CTGTGGGAAG CTGCAGACGA TATTTCTGAA ATATCCCATG
    GAGGGGGTGC CCGGAGCCCG GATGCCAGTA CAAATACGGG TGAAACTCTG GTTTGGGCTT
    TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGAA AGCTGTCCGT CTTTGCTGAA
    ACTTATGAGA ACCAGACCAA ACTGGCCCTG GTTGGGAACT GGGGCACAAC AGGCCTCACC
    TACCCCAAGT TTTCTGATGT CATGGGCAAG ATCAAGCTAC CCAAGGATAG CTTCCGCCCC
    TCAGCTGGCT GGACCTGGGC CGGAGACTGG TTCGTGTGTC CAGAGAAGAC CCTACTCCAT
    GACATGGACG CGGGCCACCT GAGTTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTC
    CCTGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACAG ATGTGAATGG GGATAAGGTG
    CTTCCTAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA AGAGTGGTCC
    ACGGACCTCA ATCGGGCCGT CGATGAGCAA GGCTGGGAGT ACAGTATCAC CATCCCCCCG
    GATCGGAAGC CAAAGCACTG GGTTCCTGCT GAGAAGATGT ATTACACGCA CCGTCGGCGG
    CGCTGGGTCC GCCTGCGCAG AAGGGACCTC AGCCAGATGG AGGCATTGAA GAGGCACAGA
    CAGGCAGAGG CAGAGGGTGA GGGCTGGGAG TACGCCTCGC TCTTGGGCTG GAAGTTCCAC
    CTGGAGTACC GCAAGACTGA TGCTTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAACCG
    CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT GCCCTTGAGG GGGCTCTGGG TGGTGTGGTA
    GATGACAGGA GTGAAGATTC CGTGTCCGTG TCCACCTTGA GCTTTGGAGT GAACAGACCC
    ACGATTTCCT GCATCTTTGA TTATGGGAAC CGCTACCACC TACGCTGCTA CATGTACCAG
    GCTCGGGACC TGCCCTCGAT GGACAAGGAC TCTTTTTCTG ATCCCTACGC CATCGTCTCC
    TTCCTGCACC AGAGCCAGAA GACAGTGGTG GCGAAGAACA CTCTGAACCC CACCTGGGAC
    CAGACGCTTA TCTTCTATGA GATTGAGATC TTTGGTGAGC CAGCCAGTAT CGCTGAGCAG
    CCGCCCAGCA TTGTGGTGGA GCTGTATGAC CACGACACCT ACGGTGCAGA TGAGTTTATG
    GGACGCTGCA TTTGTCAGCC CAGCCTGGAG CGGACACCCC GGCTGGCCTG GTTCCCACTG
    ATTAGGGGCA GCCAGTCGGC GGGGGAACTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAA
    AAGCCGGCCA TTCACCATAT TCCTGGTTTT GAGGTACAGG ATATGTCAGG AATCCTGGAA
    GAGCTGTGTG CACCTGCCTT CTTCCTCCAG GGCCTCCTCC AAACGTCAGA GGACATAGAC
    CTGCCCTACC CGCCACCTCA GAGGGAGGCC AACATCTTCA TGGTCCCCCA GAACATCAAG
    CCTGTTCTCC AGCGTACTGC CATAGAGATC CTGGCGTGGG GCCTGCGGAA CATGAAGAGC
    TACCAGCTAG CCAGCATCTC CTCCCCTAGC CTTGTGGTAG AGTGCGGGGG CCAGACAGTG
    CAGTCCTGTG TCATCAAGAA CCTCCGGAAG AACCCCAACT TCGACATCTG TACCCTTTTC
    ATGGAAGTGA TGCTGCCCAA GGAGGAGGTC TACTGTCCCT CCATCGTGAT CAAGGTCATT
    GATAACCGCC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATCCG TTCCCTGGAG
    AGCTTCATGT GTGACCTGTA CTCCGAGGAG AATCCGTCCC CACAGGGCGG CCCAGATGAT
    GTGAGTTTAC TCAGTCCTGG GGAAGACGTG CTCATTGACA TCGATGACAA GGAGCCCCTC
    ATCCCCATGC AGCTTGCAGA TGGTCTGTCG GGCTCTGCCC CCACTAACAT GGCATCTTCT
    CCATCCAGTC CTCATAAGGC CCTCCTGGAG GAAGAGTTCA TCGACTGGTG GAGCAAATTC
    TTTGCCTCCA CAGGGGAGAG GGAAAAGTGC GGCTCATACT TGGAGAAGGG CTTTGACACT
    CTAAAGGTCT ATGACACACA ACTGGAGAAC GTGGAGGCCT TTGAGGGCCT GTCTGACTTT
    TGTAACACCT TCAAGCTCTA CCGGGGCAAG ACTCAGGAGG AGACAGAGGA CCCATCTGTT
    ATTGGGGAGT TTAAGGGCCT CTTCAAAATC TATCCCCTCC CAGAAGACCC GGCTGTTCCC
    ATGCCCCCGA GACAATTCCA CCAGCTGGCT GCCCAAGGGC CTCAGGAGTG CCTGGTCCGC
    ATCTATATCA TCCGAGCATT TGGCCTTCAG CCCAAGGACC CCAATGGGAA GTGTGATCCT
    TACATCAAGA TCTCCATAGG GAAGAAATCA GTGAGTGACC AGGATAACTA CATTCCCTGT
    ACTCTGGAGC CTGTGTTTGG AAAGATGTTC GAGCTGACCT GCACTCTGCC TCTGGAGAAG
    GACCTGAAGA TCACACTCTA TGACTATGAC CTCCTCTCCA AAGATGAAAA GATTGGGGAG
    ACAGTCATTG ACCTGGAGGA TAGGCTGCTG TCCAAGTTTG GGGCTCGCTG CGGACTCCCA
    CAGACGTACT GTGTCTCTGG ACCCAACCAG TGGAGGGACC AGCTCCGCCC CTCCCAGCTT
    CTCCACCTCT TCTGCCAGCA GCGCAGACTC AAGGCTCCTG TGTACCTCAC GGACCACGTG
    GTGTTCCAGG ATAAAGAATA CAGCATTGAA GAAATAGAGG CTGGCCGAGT CCCGAACCCA
    CACCTGGGCC CAGTGGAAGA GCGTCTGGCC TTGCATGTCC TCCAGCAGCA GGGCCTGGTC
    CCTGAGCATG TGGAGTCCCG GCCTCTCTAC AGCCCCCTGC AGCCAGACAT TGAACAGGGG
    AAGCTGCAGA TGTGGATCGA CCTATTTCCC AAGGCCCTGG GGCGTCCCGG ACCTCCCTTC
    AACATCACCC CGCGGAGAGC CAGAAGGTTT TTCCTGCGTT GTATCATCTG GAACACAAAG
    GACGTGATCC TGGACGACCT CAGCATCACA GGGGAGAAGA TGAGTGATAT TTATGTGAAG
    GGTTGGATGG TTGGCTTTGA AGAGCACAAG CAAAAGACAG ATGTGCATTA CCGGTCCCTG
    GGAGGTGAAG GCAACTTTAA CTGGAGGTTC ATTTTCCCCT TTGACTACCT GCCAGCCGAG
    CAAGTCTGCA CCGTCTCTAA GAAGGATGCT TTCTGGAGGC TGGACAAGAC TGAGAGCAAA
    ATCCCAGCAC GAGTGGTATT TCAGATCTGG GACAATGACA AGTTCTCCTT TGACGACTTT
    CTGGGCTCCT TGCAGTTGGA TCTCAACCAC ATGCCCAAGC CTGCCAAGAC AGCTGAGAAG
    TGCTCCTTGG ACCAGCTGGA TGACACTTTC CACCCGGAGT GGTTTGTGTC CCTTTTTGAA
    CAGAAAACAG TTAAGGGCTG GTGGCCCTGC GTAGCAGAAG AGGGCGAGAA GAAGATCCTG
    GCAGGCAAGC TGGAAATGAC CTTGGAGATC GTAGCAGAGA GTGAGCATGA GGAGCGGCCT
    GCTGGCCAGG GCCGGGATGA ACCCAACATG AACCCCAAGC TCGAGGACCC AAGGCGCCCT
    GACACCTCCT TCCTCTGGTT CACCTCTCCA TACAAGACTA TGAAGTTCAT CCTGTGGCGG
    CGGTTCCGAT GTGCCATTAT CCTCTTCATC ATTCTCTTCA TCCTGCTACT ATTCTTAGGC
    ATCTTCATCT ATGCTTTCCC GAACTACGCT GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022260562.1
    CDS225..6464
    Translation

    Target ORF information:

    RefSeq Version XM_022404854.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris dysferlin (DYSF), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022404854.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGGGAGGA ACAGGTTCTT AGGGGAAGCC CAAGTCCCAC TCCAGGAGGT CCTCGCCACT 
    CCTAGTCTGT CTGCCAGCTT CAATGCCCCC CTCCTGGACG CCAAGAAGCA GCCCACGGGG
    GCCTCGCTGG TCCTGCAGGT GTCCTACACT CCACTCCCTG GAGCCGTGCC CCTGTTCCCA
    CCTGCTGCAT CTCTGGAACC CTCCCCGACT CTGCCTGACA TGGATATGGG GGCCGGTGGG
    GGACAGAGCC GGGCTGAGAC TTGGTCCCTG CTCAGTGACA GCACCGTGGA TACAAGATAC
    TCTGGAAAGA AGTGGCTGGC CCCCACGGAC ACAGGAGGAG AGGAGGACAC TGAGGACCAG
    GGGCTCACTG GAGACGAGGG AGAGCCATTA CTGGATCAGA GTGGCGTCCC AGGCCCTGGG
    GCTCCCACCA CCCCGAGGAA GCCACCTTCC CATCTGCCCC CCTACCACCC TGGGAGCAAG
    AGGAAAAGAA GTGCTCCCGC AGCCAGAAAG CTGCTGTCCG ACAAACCGCA GGACTTCCAG
    ATCAGAGTGC AAGTGATCGA GGGCCGCCAG CTGCCAGGGG TGAATATCAA GCCTGTGATC
    AAGGTCACTG CTGCTGGGCA GACCAAGAGG ACACGGATCC ACAAGGGAAA CAGCCCACTA
    TTTAACGAGA CTCTTTTCTT CAATGTGTTT GACTCTCCTT CGGAGCTGTT TGATGAGCCC
    ATCTTTATCA CGGTCGTAGA CTCCCGTTCT CTCAGGACAG ATGCTCTCAT TGGGGAATTC
    CGGATGGATG TGGGTACCAT CTACAGAGAG CCCCGACATG CTTATCTGAG GAAGTGGCTG
    CTACTCTCCA ACCCTGATGA CTTCTCTGTT GGGGCCAGAG GTTACCTGAA AGCAAGCCTT
    TATGTGCTGG GGCCCGGAGA TGAAGCGCCT CTGGAGAGAA AGGACCCCTC TGAAGACAAG
    GAGGACATTG AAAACAATCT GCTCAGGCCA ACTGGCGTGG CCCTGCGAGG AGCCCACTTC
    TGCCTGAAGA TCTTCAGAGC GGAGGACTTA CCACAAATGG ATGATGCCGT GATGGACAAT
    GTGAAGCAGA TCTTTGGCTT TGACAGCAAC AAGAAGAATT TGGTAGATCC CTTCATGGAA
    GTCAGCTTTG CGGGGAAAAT GCTCTGCAGC AAGATCCTGG AGAAGACAGC CAACCCTCAG
    TGGAACCAGA GCATCACTCT GCCTGCCATG TTTCCCTCCA TGTGTGAAAA AATGAAGATT
    CGCGTCATGG ACTGGGACCG CCTTACCCAC AATGACATCG TGGCCACCAC CTACCTGAGT
    ATGTCGAAAA TCTCTGCCCC TGGAGGAGAA ATAGAAGAGG AGCCTGCAGG TGTGGTCAAG
    CCTTCTCCAG CCACGGACCT GGATGACCAT CTGGGCTTCC TTCCCACCTT CGGGCCCTGC
    TTCGTCAATC TCTATGGCAG CCCCAGGGAA TTCACTGGCT TCCCGGACCC CTACGCGGAA
    CTCAACATGG GCAAGGGGGA AGGCGTGGCC TACCGAGGTC GGCTTCTGCT CTCCCTGGAG
    ACCAAGCTGG TGGAGCACAG CGGCGAGCAA AGGGTGGAGG ACCTCCCTGC TGATGACATC
    CTTCGGGTGG AGAAATACCT CCGGAGGCGC AAGTACTCTC TCTTTGCCGC CTTCTACTCG
    GCCACCATGC TCCAGGATGT CGACGATGCC ATCCAGTTTG AGGTCAGCAT TGGGAACTAT
    GGCAACAAGT TCGACACGAC CTGTCTGCCA CTGGCCTCCA CCACCCAGTA CAGCCGGGCA
    GTCTTTGATG GGTGCCACTA CTACTACTTG CCCTGGGGCA ATGTGAAGCC TGTGGTGGTG
    CTGTCATCCT ACTGGGAAGA CATCAGCCAC AGAGTCGAGG CTCAGAACCA GCTGCTCAGG
    ATCGCTGACC ATCTGGAAGC TGGCCTGGAG CAAGTCCACT TGGCTTTAAA GGCGCAGTTC
    AACACAGAGG ATGTGGACTC CCTGTTGGCC CAGCTGATGG ATGAGCTCAT CTCAGACTGC
    AGCCAGCCCC TGGGCAATGT CCAAGAGATA CCCTCTGCCA CCCACCTGGA CCGGCACCTG
    TACAGGCTGC GCACCCGTCA CCTAGGCCAG ATTACTGAGG CGGCCCTGGC TCTGAAACTC
    GGGCATGGCA AGCTCCCTGC CGCCCTGGAG CAGGCTGAAG ACTGGCTCCT GCGTCTCCGT
    GCCCTGGCTG ATGAGCCCCA GAACAGCCTG CCCGACATCA TCATCTGGAT GCTGCAGGGA
    AACAAGCGAG TGGCGTACCA GCGGGTGCCT GCGCACGAGG TCCTCTTCTC CCGGCGGGGC
    GCCAGCTACT GTGGCAAGAA CTGTGGGAAG CTGCAGACGA TATTTCTGAA ATATCCCATG
    GAGGGGGTGC CCGGAGCCCG GATGCCAGTA CAAATACGGG TGAAACTCTG GTTTGGGCTT
    TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGAA AGCTGTCCGT CTTTGCTGAA
    ACTTATGAGA ACCAGACCAA ACTGGCCCTG GTTGGGAACT GGGGCACAAC AGGCCTCACC
    TACCCCAAGT TTTCTGATGT CATGGGCAAG ATCAAGCTAC CCAAGGATAG CTTCCGCCCC
    TCAGCTGGCT GGACCTGGGC CGGAGACTGG TTCGTGTGTC CAGAGAAGAC CCTACTCCAT
    GACATGGACG CGGGCCACCT GAGTTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTC
    CCTGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACAG ATGTGAATGG GGATAAGGTG
    CTTCCTAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA AGAGTGGTCC
    ACGGACCTCA ATCGGGCCGT CGATGAGCAA GGCTGGGAGT ACAGTATCAC CATCCCCCCG
    GATCGGAAGC CAAAGCACTG GGTTCCTGCT GAGAAGATGT ATTACACGCA CCGTCGGCGG
    CGCTGGGTCC GCCTGCGCAG AAGGGACCTC AGCCAGATGG AGGCATTGAA GAGGCACAGA
    CAGGCAGAGG CAGAGGGTGA GGGCTGGGAG TACGCCTCGC TCTTGGGCTG GAAGTTCCAC
    CTGGAGTACC GCAAGACTGA TGCTTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAACCG
    CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT GCCCTTGAGG GGGCTCTGGG TGGTGTGGTA
    GATGACAGGA GTGAAGATTC CGTGTCCGTG TCCACCTTGA GCTTTGGAGT GAACAGACCC
    ACGATTTCCT GCATCTTTGA TTATGGGAAC CGCTACCACC TACGCTGCTA CATGTACCAG
    GCTCGGGACC TGCCCTCGAT GGACAAGGAC TCTTTTTCTG ATCCCTACGC CATCGTCTCC
    TTCCTGCACC AGAGCCAGAA GACAGTGGTG GCGAAGAACA CTCTGAACCC CACCTGGGAC
    CAGACGCTTA TCTTCTATGA GATTGAGATC TTTGGTGAGC CAGCCAGTAT CGCTGAGCAG
    CCGCCCAGCA TTGTGGTGGA GCTGTATGAC CACGACACCT ACGGTGCAGA TGAGTTTATG
    GGACGCTGCA TTTGTCAGCC CAGCCTGGAG CGGACACCCC GGCTGGCCTG GTTCCCACTG
    ATTAGGGGCA GCCAGTCGGC GGGGGAACTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAA
    AAGCCGGCCA TTCACCATAT TCCTGGTTTT GAGGTACAGG ATATGTCAGG AATCCTGGAA
    GAGCTGTGTG CACCTGCCTT CTTCCTCCAG GGCCTCCTCC AAACGTCAGA GGACATAGAC
    CTGCCCTACC CGCCACCTCA GAGGGAGGCC AACATCTTCA TGGTCCCCCA GAACATCAAG
    CCTGTTCTCC AGCGTACTGC CATAGAGATC CTGGCGTGGG GCCTGCGGAA CATGAAGAGC
    TACCAGCTAG CCAGCATCTC CTCCCCTAGC CTTGTGGTAG AGTGCGGGGG CCAGACAGTG
    CAGTCCTGTG TCATCAAGAA CCTCCGGAAG AACCCCAACT TCGACATCTG TACCCTTTTC
    ATGGAAGTGA TGCTGCCCAA GGAGGAGGTC TACTGTCCCT CCATCGTGAT CAAGGTCATT
    GATAACCGCC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATCCG TTCCCTGGAG
    AGCTTCATGT GTGACCTGTA CTCCGAGGAG AATCCGTCCC CACAGGGCGG CCCAGATGAT
    GTGAGTTTAC TCAGTCCTGG GGAAGACGTG CTCATTGACA TCGATGACAA GGAGCCCCTC
    ATCCCCATGC AGCTTGCAGA TGGTCTGTCG GGCTCTGCCC CCACTAACAT GGCATCTTCT
    CCATCCAGTC CTCATAAGGC CCTCCTGGAG GAAGAGTTCA TCGACTGGTG GAGCAAATTC
    TTTGCCTCCA CAGGGGAGAG GGAAAAGTGC GGCTCATACT TGGAGAAGGG CTTTGACACT
    CTAAAGGTCT ATGACACACA ACTGGAGAAC GTGGAGGCCT TTGAGGGCCT GTCTGACTTT
    TGTAACACCT TCAAGCTCTA CCGGGGCAAG ACTCAGGAGG AGACAGAGGA CCCATCTGTT
    ATTGGGGAGT TTAAGGGCCT CTTCAAAATC TATCCCCTCC CAGAAGACCC GGCTGTTCCC
    ATGCCCCCGA GACAATTCCA CCAGCTGGCT GCCCAAGGGC CTCAGGAGTG CCTGGTCCGC
    ATCTATATCA TCCGAGCATT TGGCCTTCAG CCCAAGGACC CCAATGGGAA GTGTGATCCT
    TACATCAAGA TCTCCATAGG GAAGAAATCA GTGAGTGACC AGGATAACTA CATTCCCTGT
    ACTCTGGAGC CTGTGTTTGG AAAGATGTTC GAGCTGACCT GCACTCTGCC TCTGGAGAAG
    GACCTGAAGA TCACACTCTA TGACTATGAC CTCCTCTCCA AAGATGAAAA GATTGGGGAG
    ACAGTCATTG ACCTGGAGGA TAGGCTGCTG TCCAAGTTTG GGGCTCGCTG CGGACTCCCA
    CAGACGTACT GTGTCTCTGG ACCCAACCAG TGGAGGGACC AGCTCCGCCC CTCCCAGCTT
    CTCCACCTCT TCTGCCAGCA GCGCAGACTC AAGGCTCCTG TGTACCTCAC GGACCACGTG
    GTGTTCCAGG ATAAAGAATA CAGCATTGAA GAAATAGAGG CTGGCCGAGT CCCGAACCCA
    CACCTGGGCC CAGTGGAAGA GCGTCTGGCC TTGCATGTCC TCCAGCAGCA GGGCCTGGTC
    CCTGAGCATG TGGAGTCCCG GCCTCTCTAC AGCCCCCTGC AGCCAGACAT TGAACAGGGG
    AAGCTGCAGA TGTGGATCGA CCTATTTCCC AAGGCCCTGG GGCGTCCCGG ACCTCCCTTC
    AACATCACCC CGCGGAGAGC CAGAAGGTTT TTCCTGCGTT GTATCATCTG GAACACAAAG
    GACGTGATCC TGGACGACCT CAGCATCACA GGGGAGAAGA TGAGTGATAT TTATGTGAAG
    GGTTGGATGG TTGGCTTTGA AGAGCACAAG CAAAAGACAG ATGTGCATTA CCGGTCCCTG
    GGAGGTGAAG GCAACTTTAA CTGGAGGTTC ATTTTCCCCT TTGACTACCT GCCAGCCGAG
    CAAGTCTGCA CCGTCTCTAA GAAGGATGCT TTCTGGAGGC TGGACAAGAC TGAGAGCAAA
    ATCCCAGCAC GAGTGGTATT TCAGATCTGG GACAATGACA AGTTCTCCTT TGACGACTTT
    CTGGGCTCCT TGCAGTTGGA TCTCAACCAC ATGCCCAAGC CTGCCAAGAC AGCTGAGAAG
    TGCTCCTTGG ACCAGCTGGA TGACACTTTC CACCCGGAGT GGTTTGTGTC CCTTTTTGAA
    CAGAAAACAG TTAAGGGCTG GTGGCCCTGC GTAGCAGAAG AGGGCGAGAA GAAGATCCTG
    GCAGGCAAGC TGGAAATGAC CTTGGAGATC GTAGCAGAGA GTGAGCATGA GGAGCGGCCT
    GCTGGCCAGG GCCGGGATGA ACCCAACATG AACCCCAAGC TCGAGGACCC AAGGCGCCCT
    GACACCTCCT TCCTCTGGTT CACCTCTCCA TACAAGACTA TGAAGTTCAT CCTGTGGCGG
    CGGTTCCGAT GTGCCATTAT CCTCTTCATC ATTCTCTTCA TCCTGCTACT ATTCTTAGGC
    ATCTTCATCT ATGCTTTCCC GAACTACGCT GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe19206
    Clone ID Related Accession (Same CDS sequence) XM_003432234.4 , XM_003432234.3
    Accession Version XM_003432234.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6369bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product dysferlin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003432234.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG
    CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC
    GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG
    AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT
    CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG
    ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT
    GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT
    TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC
    GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT
    ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT
    GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC
    GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC
    AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC
    AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC
    ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG
    GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT
    GCCCCTGGAG GAGAAATAGA AGTGGATGAC CATCTGGGCT TCCTTCCCAC CTTCGGGCCC
    TGCTTCGTCA ATCTCTATGG CAGCCCCAGG GAATTCACTG GCTTCCCGGA CCCCTACGCG
    GAACTCAACA TGGGCAAGGG GGAAGGCGTG GCCTACCGAG GTCGGCTTCT GCTCTCCCTG
    GAGACCAAGC TGGTGGAGCA CAGCGGCGAG CAAAGGGTGG AGGACCTCCC TGCTGATGAC
    ATCCTTCGGG TGGAGAAATA CCTCCGGAGG CGCAAGTACT CTCTCTTTGC CGCCTTCTAC
    TCGGCCACCA TGCTCCAGGA TGTCGACGAT GCCATCCAGT TTGAGGTCAG CATTGGGAAC
    TATGGCAACA AGTTCGACAC GACCTGTCTG CCACTGGCCT CCACCACCCA GTACAGCCGG
    GCAGTCTTTG ATGGGTGCCA CTACTACTAC TTGCCCTGGG GCAATGTGAA GCCTGTGGTG
    GTGCTGTCAT CCTACTGGGA AGACATCAGC CACAGAGTCG AGGCTCAGAA CCAGCTGCTC
    AGGATCGCTG ACCATCTGGA AGCTGGCCTG GAGCAAGTCC ACTTGGCTTT AAAGGCGCAG
    TTCAACACAG AGGATGTGGA CTCCCTGTTG GCCCAGCTGA TGGATGAGCT CATCTCAGAC
    TGCAGCCAGC CCCTGGGCAA TGTCCAAGAG ATACCCTCTG CCACCCACCT GGACCGGCAC
    CTGTACAGGC TGCGCACCCG TCACCTAGGC CAGATTACTG AGGCGGCCCT GGCTCTGAAA
    CTCGGGCATG GCAAGCTCCC TGCCGCCCTG GAGCAGGCTG AAGACTGGCT CCTGCGTCTC
    CGTGCCCTGG CTGATGAGCC CCAGAACAGC CTGCCCGACA TCATCATCTG GATGCTGCAG
    GGAAACAAGC GAGTGGCGTA CCAGCGGGTG CCTGCGCACG AGGTCCTCTT CTCCCGGCGG
    GGCGCCAGCT ACTGTGGCAA GAACTGTGGG AAGCTGCAGA CGATATTTCT GAAATATCCC
    ATGGAGGGGG TGCCCGGAGC CCGGATGCCA GTACAAATAC GGGTGAAACT CTGGTTTGGG
    CTTTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GAAAGCTGTC CGTCTTTGCT
    GAAACTTATG AGAACCAGAC CAAACTGGCC CTGGTTGGGA ACTGGGGCAC AACAGGCCTC
    ACCTACCCCA AGTTTTCTGA TGTCATGGGC AAGATCAAGC TACCCAAGGA TAGCTTCCGC
    CCCTCAGCTG GCTGGACCTG GGCCGGAGAC TGGTTCGTGT GTCCAGAGAA GACCCTACTC
    CATGACATGG ACGCGGGCCA CCTGAGTTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG
    CTCCCTGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CAGATGTGAA TGGGGATAAG
    GTGCTTCCTA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAAGAGTGG
    TCCACGGACC TCAATCGGGC CGTCGATGAG CAAGGCTGGG AGTACAGTAT CACCATCCCC
    CCGGATCGGA AGCCAAAGCA CTGGGTTCCT GCTGAGAAGA TGTATTACAC GCACCGTCGG
    CGGCGCTGGG TCCGCCTGCG CAGAAGGGAC CTCAGCCAGA TGGAGGCATT GAAGAGGCAC
    AGACAGGCAG AGGCAGAGGG TGAGGGCTGG GAGTACGCCT CGCTCTTGGG CTGGAAGTTC
    CACCTGGAGT ACCGCAAGAC TGATGCTTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAA
    CCGCTGGAGA AGACGGGGCC TGCAGCCGTG TTTGCCCTTG AGGGGGCTCT GGGTGGTGTG
    GTAGATGACA GGAGTGAAGA TTCCGTGTCC GTGTCCACCT TGAGCTTTGG AGTGAACAGA
    CCCACGATTT CCTGCATCTT TGATTATGGG AACCGCTACC ACCTACGCTG CTACATGTAC
    CAGGCTCGGG ACCTGCCCTC GATGGACAAG GACTCTTTTT CTGATCCCTA CGCCATCGTC
    TCCTTCCTGC ACCAGAGCCA GAAGACAGTG GTGGCGAAGA ACACTCTGAA CCCCACCTGG
    GACCAGACGC TTATCTTCTA TGAGATTGAG ATCTTTGGTG AGCCAGCCAG TATCGCTGAG
    CAGCCGCCCA GCATTGTGGT GGAGCTGTAT GACCACGACA CCTACGGTGC AGATGAGTTT
    ATGGGACGCT GCATTTGTCA GCCCAGCCTG GAGCGGACAC CCCGGCTGGC CTGGTTCCCA
    CTGATTAGGG GCAGCCAGTC GGCGGGGGAA CTGCTGGCCT CTTTTGAGCT CATCCAGAGA
    GAAAAGCCGG CCATTCACCA TATTCCTGGT TTTGAGGTAC AGGATATGTC AGGAATCCTG
    GAAGAGTCAG AGGACATAGA CCTGCCCTAC CCGCCACCTC AGAGGGAGGC CAACATCTTC
    ATGGTCCCCC AGAACATCAA GCCTGTTCTC CAGCGTACTG CCATAGAGAT CCTGGCGTGG
    GGCCTGCGGA ACATGAAGAG CTACCAGCTA GCCAGCATCT CCTCCCCTAG CCTTGTGGTA
    GAGTGCGGGG GCCAGACAGT GCAGTCCTGT GTCATCAAGA ACCTCCGGAA GAACCCCAAC
    TTCGACATCT GTACCCTTTT CATGGAAGTG ATGCTGCCCA AGGAGGAGGT CTACTGTCCC
    TCCATCGTGA TCAAGGTCAT TGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG
    TGTACCATCC GTTCCCTGGA GAGCTTCATG TGTGACCTGT ACTCCGAGGA GAATCCGTCC
    CCACAGGGCG GCCCAGATGA TGTGAGTTTA CTCAGTCCTG GGGAAGACGT GCTCATTGAC
    ATCGATGACA AGGAGCCCCT CATCCCCATG CAGCTTGCAG ATGGTCTGTC GGGCTCTGCC
    CCCACTAACA TGGCATCTTC TCCATCCAGT CCTCATGAGG AAGAGTTCAT CGACTGGTGG
    AGCAAATTCT TTGCCTCCAC AGGGGAGAGG GAAAAGTGCG GCTCATACTT GGAGAAGGGC
    TTTGACACTC TAAAGGTCTA TGACACACAA CTGGAGAACG TGGAGGCCTT TGAGGGCCTG
    TCTGACTTTT GTAACACCTT CAAGCTCTAC CGGGGCAAGA CTCAGGAGGA GACAGAGGAC
    CCATCTGTTA TTGGGGAGTT TAAGGGCCTC TTCAAAATCT ATCCCCTCCC AGAAGACCCG
    GCTGTTCCCA TGCCCCCGAG ACAATTCCAC CAGCTGGCTG CCCAAGGGCC TCAGGAGTGC
    CTGGTCCGCA TCTATATCAT CCGAGCATTT GGCCTTCAGC CCAAGGACCC CAATGGGAAG
    TGTGATCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC
    ATTCCCTGTA CTCTGGAGCC TGTGTTTGGA AAGATGTTCG AGCTGACCTG CACTCTGCCT
    CTGGAGAAGG ACCTGAAGAT CACACTCTAT GACTATGACC TCCTCTCCAA AGATGAAAAG
    ATTGGGGAGA CAGTCATTGA CCTGGAGGAT AGGCTGCTGT CCAAGTTTGG GGCTCGCTGC
    GGACTCCCAC AGACGTACTG TGTCTCTGGA CCCAACCAGT GGAGGGACCA GCTCCGCCCC
    TCCCAGCTTC TCCACCTCTT CTGCCAGCAG CGCAGACTCA AGGCTCCTGT GTACCTCACG
    GACCACGTGG TGTTCCAGGA TAAAGAATAC AGCATTGAAG AAATAGAGGC TGGCCGAGTC
    CCGAACCCAC ACCTGGGCCC AGTGGAAGAG CGTCTGGCCT TGCATGTCCT CCAGCAGCAG
    GGCCTGGTCC CTGAGCATGT GGAGTCCCGG CCTCTCTACA GCCCCCTGCA GCCAGACATT
    GAACAGGGGA AGCTGCAGAT GTGGATCGAC CTATTTCCCA AGGCCCTGGG GCGTCCCGGA
    CCTCCCTTCA ACATCACCCC GCGGAGAGCC AGAAGGTTTT TCCTGCGTTG TATCATCTGG
    AACACAAAGG ACGTGATCCT GGACGACCTC AGCATCACAG GGGAGAAGAT GAGTGATATT
    TATGTGAAGG GTTGGATGGT TGGCTTTGAA GAGCACAAGC AAAAGACAGA TGTGCATTAC
    CGGTCCCTGG GAGGTGAAGG CAACTTTAAC TGGAGGTTCA TTTTCCCCTT TGACTACCTG
    CCAGCCGAGC AAGTCTGCAC CGTCTCTAAG AAGGATGCTT TCTGGAGGCT GGACAAGACT
    GAGAGCAAAA TCCCAGCACG AGTGGTATTT CAGATCTGGG ACAATGACAA GTTCTCCTTT
    GACGACTTTC TGGGCTCCTT GCAGTTGGAT CTCAACCACA TGCCCAAGCC TGCCAAGACA
    GCTGAGAAGT GCTCCTTGGA CCAGCTGGAT GACACTTTCC ACCCGGAGTG GTTTGTGTCC
    CTTTTTGAAC AGAAAACAGT TAAGGGCTGG TGGCCCTGCG TAGCAGAAGA GGGCGAGAAG
    AAGATCCTGG CAGGCAAGCT GGAAATGACC TTGGAGATCG TAGCAGAGAG TGAGCATGAG
    GAGCGGCCTG CTGGCCAGGG CCGGGATGAA CCCAACATGA ACCCCAAGCT CGAGGACCCA
    AGGCGCCCTG ACACCTCCTT CCTCTGGTTC ACCTCTCCAT ACAAGACTAT GAAGTTCATC
    CTGTGGCGGC GGTTCCGATG TGCCATTATC CTCTTCATCA TTCTCTTCAT CCTGCTACTA
    TTCTTAGGCA TCTTCATCTA TGCTTTCCCG AACTACGCTG CCATGAAGCT GGTGAAGCCC
    TTCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003432282.1
    CDS23..6391
    Translation

    Target ORF information:

    RefSeq Version XM_003432234.4
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris dysferlin (DYSF), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003432234.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG
    CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC
    GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG
    AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT
    CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG
    ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT
    GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT
    TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC
    GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT
    ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT
    GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC
    GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC
    AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC
    AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC
    ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG
    GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT
    GCCCCTGGAG GAGAAATAGA AGTGGATGAC CATCTGGGCT TCCTTCCCAC CTTCGGGCCC
    TGCTTCGTCA ATCTCTATGG CAGCCCCAGG GAATTCACTG GCTTCCCGGA CCCCTACGCG
    GAACTCAACA TGGGCAAGGG GGAAGGCGTG GCCTACCGAG GTCGGCTTCT GCTCTCCCTG
    GAGACCAAGC TGGTGGAGCA CAGCGGCGAG CAAAGGGTGG AGGACCTCCC TGCTGATGAC
    ATCCTTCGGG TGGAGAAATA CCTCCGGAGG CGCAAGTACT CTCTCTTTGC CGCCTTCTAC
    TCGGCCACCA TGCTCCAGGA TGTCGACGAT GCCATCCAGT TTGAGGTCAG CATTGGGAAC
    TATGGCAACA AGTTCGACAC GACCTGTCTG CCACTGGCCT CCACCACCCA GTACAGCCGG
    GCAGTCTTTG ATGGGTGCCA CTACTACTAC TTGCCCTGGG GCAATGTGAA GCCTGTGGTG
    GTGCTGTCAT CCTACTGGGA AGACATCAGC CACAGAGTCG AGGCTCAGAA CCAGCTGCTC
    AGGATCGCTG ACCATCTGGA AGCTGGCCTG GAGCAAGTCC ACTTGGCTTT AAAGGCGCAG
    TTCAACACAG AGGATGTGGA CTCCCTGTTG GCCCAGCTGA TGGATGAGCT CATCTCAGAC
    TGCAGCCAGC CCCTGGGCAA TGTCCAAGAG ATACCCTCTG CCACCCACCT GGACCGGCAC
    CTGTACAGGC TGCGCACCCG TCACCTAGGC CAGATTACTG AGGCGGCCCT GGCTCTGAAA
    CTCGGGCATG GCAAGCTCCC TGCCGCCCTG GAGCAGGCTG AAGACTGGCT CCTGCGTCTC
    CGTGCCCTGG CTGATGAGCC CCAGAACAGC CTGCCCGACA TCATCATCTG GATGCTGCAG
    GGAAACAAGC GAGTGGCGTA CCAGCGGGTG CCTGCGCACG AGGTCCTCTT CTCCCGGCGG
    GGCGCCAGCT ACTGTGGCAA GAACTGTGGG AAGCTGCAGA CGATATTTCT GAAATATCCC
    ATGGAGGGGG TGCCCGGAGC CCGGATGCCA GTACAAATAC GGGTGAAACT CTGGTTTGGG
    CTTTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GAAAGCTGTC CGTCTTTGCT
    GAAACTTATG AGAACCAGAC CAAACTGGCC CTGGTTGGGA ACTGGGGCAC AACAGGCCTC
    ACCTACCCCA AGTTTTCTGA TGTCATGGGC AAGATCAAGC TACCCAAGGA TAGCTTCCGC
    CCCTCAGCTG GCTGGACCTG GGCCGGAGAC TGGTTCGTGT GTCCAGAGAA GACCCTACTC
    CATGACATGG ACGCGGGCCA CCTGAGTTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG
    CTCCCTGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CAGATGTGAA TGGGGATAAG
    GTGCTTCCTA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAAGAGTGG
    TCCACGGACC TCAATCGGGC CGTCGATGAG CAAGGCTGGG AGTACAGTAT CACCATCCCC
    CCGGATCGGA AGCCAAAGCA CTGGGTTCCT GCTGAGAAGA TGTATTACAC GCACCGTCGG
    CGGCGCTGGG TCCGCCTGCG CAGAAGGGAC CTCAGCCAGA TGGAGGCATT GAAGAGGCAC
    AGACAGGCAG AGGCAGAGGG TGAGGGCTGG GAGTACGCCT CGCTCTTGGG CTGGAAGTTC
    CACCTGGAGT ACCGCAAGAC TGATGCTTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAA
    CCGCTGGAGA AGACGGGGCC TGCAGCCGTG TTTGCCCTTG AGGGGGCTCT GGGTGGTGTG
    GTAGATGACA GGAGTGAAGA TTCCGTGTCC GTGTCCACCT TGAGCTTTGG AGTGAACAGA
    CCCACGATTT CCTGCATCTT TGATTATGGG AACCGCTACC ACCTACGCTG CTACATGTAC
    CAGGCTCGGG ACCTGCCCTC GATGGACAAG GACTCTTTTT CTGATCCCTA CGCCATCGTC
    TCCTTCCTGC ACCAGAGCCA GAAGACAGTG GTGGCGAAGA ACACTCTGAA CCCCACCTGG
    GACCAGACGC TTATCTTCTA TGAGATTGAG ATCTTTGGTG AGCCAGCCAG TATCGCTGAG
    CAGCCGCCCA GCATTGTGGT GGAGCTGTAT GACCACGACA CCTACGGTGC AGATGAGTTT
    ATGGGACGCT GCATTTGTCA GCCCAGCCTG GAGCGGACAC CCCGGCTGGC CTGGTTCCCA
    CTGATTAGGG GCAGCCAGTC GGCGGGGGAA CTGCTGGCCT CTTTTGAGCT CATCCAGAGA
    GAAAAGCCGG CCATTCACCA TATTCCTGGT TTTGAGGTAC AGGATATGTC AGGAATCCTG
    GAAGAGTCAG AGGACATAGA CCTGCCCTAC CCGCCACCTC AGAGGGAGGC CAACATCTTC
    ATGGTCCCCC AGAACATCAA GCCTGTTCTC CAGCGTACTG CCATAGAGAT CCTGGCGTGG
    GGCCTGCGGA ACATGAAGAG CTACCAGCTA GCCAGCATCT CCTCCCCTAG CCTTGTGGTA
    GAGTGCGGGG GCCAGACAGT GCAGTCCTGT GTCATCAAGA ACCTCCGGAA GAACCCCAAC
    TTCGACATCT GTACCCTTTT CATGGAAGTG ATGCTGCCCA AGGAGGAGGT CTACTGTCCC
    TCCATCGTGA TCAAGGTCAT TGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG
    TGTACCATCC GTTCCCTGGA GAGCTTCATG TGTGACCTGT ACTCCGAGGA GAATCCGTCC
    CCACAGGGCG GCCCAGATGA TGTGAGTTTA CTCAGTCCTG GGGAAGACGT GCTCATTGAC
    ATCGATGACA AGGAGCCCCT CATCCCCATG CAGCTTGCAG ATGGTCTGTC GGGCTCTGCC
    CCCACTAACA TGGCATCTTC TCCATCCAGT CCTCATGAGG AAGAGTTCAT CGACTGGTGG
    AGCAAATTCT TTGCCTCCAC AGGGGAGAGG GAAAAGTGCG GCTCATACTT GGAGAAGGGC
    TTTGACACTC TAAAGGTCTA TGACACACAA CTGGAGAACG TGGAGGCCTT TGAGGGCCTG
    TCTGACTTTT GTAACACCTT CAAGCTCTAC CGGGGCAAGA CTCAGGAGGA GACAGAGGAC
    CCATCTGTTA TTGGGGAGTT TAAGGGCCTC TTCAAAATCT ATCCCCTCCC AGAAGACCCG
    GCTGTTCCCA TGCCCCCGAG ACAATTCCAC CAGCTGGCTG CCCAAGGGCC TCAGGAGTGC
    CTGGTCCGCA TCTATATCAT CCGAGCATTT GGCCTTCAGC CCAAGGACCC CAATGGGAAG
    TGTGATCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC
    ATTCCCTGTA CTCTGGAGCC TGTGTTTGGA AAGATGTTCG AGCTGACCTG CACTCTGCCT
    CTGGAGAAGG ACCTGAAGAT CACACTCTAT GACTATGACC TCCTCTCCAA AGATGAAAAG
    ATTGGGGAGA CAGTCATTGA CCTGGAGGAT AGGCTGCTGT CCAAGTTTGG GGCTCGCTGC
    GGACTCCCAC AGACGTACTG TGTCTCTGGA CCCAACCAGT GGAGGGACCA GCTCCGCCCC
    TCCCAGCTTC TCCACCTCTT CTGCCAGCAG CGCAGACTCA AGGCTCCTGT GTACCTCACG
    GACCACGTGG TGTTCCAGGA TAAAGAATAC AGCATTGAAG AAATAGAGGC TGGCCGAGTC
    CCGAACCCAC ACCTGGGCCC AGTGGAAGAG CGTCTGGCCT TGCATGTCCT CCAGCAGCAG
    GGCCTGGTCC CTGAGCATGT GGAGTCCCGG CCTCTCTACA GCCCCCTGCA GCCAGACATT
    GAACAGGGGA AGCTGCAGAT GTGGATCGAC CTATTTCCCA AGGCCCTGGG GCGTCCCGGA
    CCTCCCTTCA ACATCACCCC GCGGAGAGCC AGAAGGTTTT TCCTGCGTTG TATCATCTGG
    AACACAAAGG ACGTGATCCT GGACGACCTC AGCATCACAG GGGAGAAGAT GAGTGATATT
    TATGTGAAGG GTTGGATGGT TGGCTTTGAA GAGCACAAGC AAAAGACAGA TGTGCATTAC
    CGGTCCCTGG GAGGTGAAGG CAACTTTAAC TGGAGGTTCA TTTTCCCCTT TGACTACCTG
    CCAGCCGAGC AAGTCTGCAC CGTCTCTAAG AAGGATGCTT TCTGGAGGCT GGACAAGACT
    GAGAGCAAAA TCCCAGCACG AGTGGTATTT CAGATCTGGG ACAATGACAA GTTCTCCTTT
    GACGACTTTC TGGGCTCCTT GCAGTTGGAT CTCAACCACA TGCCCAAGCC TGCCAAGACA
    GCTGAGAAGT GCTCCTTGGA CCAGCTGGAT GACACTTTCC ACCCGGAGTG GTTTGTGTCC
    CTTTTTGAAC AGAAAACAGT TAAGGGCTGG TGGCCCTGCG TAGCAGAAGA GGGCGAGAAG
    AAGATCCTGG CAGGCAAGCT GGAAATGACC TTGGAGATCG TAGCAGAGAG TGAGCATGAG
    GAGCGGCCTG CTGGCCAGGG CCGGGATGAA CCCAACATGA ACCCCAAGCT CGAGGACCCA
    AGGCGCCCTG ACACCTCCTT CCTCTGGTTC ACCTCTCCAT ACAAGACTAT GAAGTTCATC
    CTGTGGCGGC GGTTCCGATG TGCCATTATC CTCTTCATCA TTCTCTTCAT CCTGCTACTA
    TTCTTAGGCA TCTTCATCTA TGCTTTCCCG AACTACGCTG CCATGAAGCT GGTGAAGCCC
    TTCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe19206
    Clone ID Related Accession (Same CDS sequence) XM_003432234.4 , XM_003432234.3
    Accession Version XM_003432234.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6369bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product dysferlin isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003432234.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG
    CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC
    GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG
    AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT
    CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG
    ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT
    GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT
    TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC
    GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT
    ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT
    GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC
    GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC
    AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC
    AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC
    ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG
    GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT
    GCCCCTGGAG GAGAAATAGA AGTGGATGAC CATCTGGGCT TCCTTCCCAC CTTCGGGCCC
    TGCTTCGTCA ATCTCTATGG CAGCCCCAGG GAATTCACTG GCTTCCCGGA CCCCTACGCG
    GAACTCAACA TGGGCAAGGG GGAAGGCGTG GCCTACCGAG GTCGGCTTCT GCTCTCCCTG
    GAGACCAAGC TGGTGGAGCA CAGCGGCGAG CAAAGGGTGG AGGACCTCCC TGCTGATGAC
    ATCCTTCGGG TGGAGAAATA CCTCCGGAGG CGCAAGTACT CTCTCTTTGC CGCCTTCTAC
    TCGGCCACCA TGCTCCAGGA TGTCGACGAT GCCATCCAGT TTGAGGTCAG CATTGGGAAC
    TATGGCAACA AGTTCGACAC GACCTGTCTG CCACTGGCCT CCACCACCCA GTACAGCCGG
    GCAGTCTTTG ATGGGTGCCA CTACTACTAC TTGCCCTGGG GCAATGTGAA GCCTGTGGTG
    GTGCTGTCAT CCTACTGGGA AGACATCAGC CACAGAGTCG AGGCTCAGAA CCAGCTGCTC
    AGGATCGCTG ACCATCTGGA AGCTGGCCTG GAGCAAGTCC ACTTGGCTTT AAAGGCGCAG
    TTCAACACAG AGGATGTGGA CTCCCTGTTG GCCCAGCTGA TGGATGAGCT CATCTCAGAC
    TGCAGCCAGC CCCTGGGCAA TGTCCAAGAG ATACCCTCTG CCACCCACCT GGACCGGCAC
    CTGTACAGGC TGCGCACCCG TCACCTAGGC CAGATTACTG AGGCGGCCCT GGCTCTGAAA
    CTCGGGCATG GCAAGCTCCC TGCCGCCCTG GAGCAGGCTG AAGACTGGCT CCTGCGTCTC
    CGTGCCCTGG CTGATGAGCC CCAGAACAGC CTGCCCGACA TCATCATCTG GATGCTGCAG
    GGAAACAAGC GAGTGGCGTA CCAGCGGGTG CCTGCGCACG AGGTCCTCTT CTCCCGGCGG
    GGCGCCAGCT ACTGTGGCAA GAACTGTGGG AAGCTGCAGA CGATATTTCT GAAATATCCC
    ATGGAGGGGG TGCCCGGAGC CCGGATGCCA GTACAAATAC GGGTGAAACT CTGGTTTGGG
    CTTTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GAAAGCTGTC CGTCTTTGCT
    GAAACTTATG AGAACCAGAC CAAACTGGCC CTGGTTGGGA ACTGGGGCAC AACAGGCCTC
    ACCTACCCCA AGTTTTCTGA TGTCATGGGC AAGATCAAGC TACCCAAGGA TAGCTTCCGC
    CCCTCAGCTG GCTGGACCTG GGCCGGAGAC TGGTTCGTGT GTCCAGAGAA GACCCTACTC
    CATGACATGG ACGCGGGCCA CCTGAGTTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG
    CTCCCTGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CAGATGTGAA TGGGGATAAG
    GTGCTTCCTA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAAGAGTGG
    TCCACGGACC TCAATCGGGC CGTCGATGAG CAAGGCTGGG AGTACAGTAT CACCATCCCC
    CCGGATCGGA AGCCAAAGCA CTGGGTTCCT GCTGAGAAGA TGTATTACAC GCACCGTCGG
    CGGCGCTGGG TCCGCCTGCG CAGAAGGGAC CTCAGCCAGA TGGAGGCATT GAAGAGGCAC
    AGACAGGCAG AGGCAGAGGG TGAGGGCTGG GAGTACGCCT CGCTCTTGGG CTGGAAGTTC
    CACCTGGAGT ACCGCAAGAC TGATGCTTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAA
    CCGCTGGAGA AGACGGGGCC TGCAGCCGTG TTTGCCCTTG AGGGGGCTCT GGGTGGTGTG
    GTAGATGACA GGAGTGAAGA TTCCGTGTCC GTGTCCACCT TGAGCTTTGG AGTGAACAGA
    CCCACGATTT CCTGCATCTT TGATTATGGG AACCGCTACC ACCTACGCTG CTACATGTAC
    CAGGCTCGGG ACCTGCCCTC GATGGACAAG GACTCTTTTT CTGATCCCTA CGCCATCGTC
    TCCTTCCTGC ACCAGAGCCA GAAGACAGTG GTGGCGAAGA ACACTCTGAA CCCCACCTGG
    GACCAGACGC TTATCTTCTA TGAGATTGAG ATCTTTGGTG AGCCAGCCAG TATCGCTGAG
    CAGCCGCCCA GCATTGTGGT GGAGCTGTAT GACCACGACA CCTACGGTGC AGATGAGTTT
    ATGGGACGCT GCATTTGTCA GCCCAGCCTG GAGCGGACAC CCCGGCTGGC CTGGTTCCCA
    CTGATTAGGG GCAGCCAGTC GGCGGGGGAA CTGCTGGCCT CTTTTGAGCT CATCCAGAGA
    GAAAAGCCGG CCATTCACCA TATTCCTGGT TTTGAGGTAC AGGATATGTC AGGAATCCTG
    GAAGAGTCAG AGGACATAGA CCTGCCCTAC CCGCCACCTC AGAGGGAGGC CAACATCTTC
    ATGGTCCCCC AGAACATCAA GCCTGTTCTC CAGCGTACTG CCATAGAGAT CCTGGCGTGG
    GGCCTGCGGA ACATGAAGAG CTACCAGCTA GCCAGCATCT CCTCCCCTAG CCTTGTGGTA
    GAGTGCGGGG GCCAGACAGT GCAGTCCTGT GTCATCAAGA ACCTCCGGAA GAACCCCAAC
    TTCGACATCT GTACCCTTTT CATGGAAGTG ATGCTGCCCA AGGAGGAGGT CTACTGTCCC
    TCCATCGTGA TCAAGGTCAT TGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG
    TGTACCATCC GTTCCCTGGA GAGCTTCATG TGTGACCTGT ACTCCGAGGA GAATCCGTCC
    CCACAGGGCG GCCCAGATGA TGTGAGTTTA CTCAGTCCTG GGGAAGACGT GCTCATTGAC
    ATCGATGACA AGGAGCCCCT CATCCCCATG CAGCTTGCAG ATGGTCTGTC GGGCTCTGCC
    CCCACTAACA TGGCATCTTC TCCATCCAGT CCTCATGAGG AAGAGTTCAT CGACTGGTGG
    AGCAAATTCT TTGCCTCCAC AGGGGAGAGG GAAAAGTGCG GCTCATACTT GGAGAAGGGC
    TTTGACACTC TAAAGGTCTA TGACACACAA CTGGAGAACG TGGAGGCCTT TGAGGGCCTG
    TCTGACTTTT GTAACACCTT CAAGCTCTAC CGGGGCAAGA CTCAGGAGGA GACAGAGGAC
    CCATCTGTTA TTGGGGAGTT TAAGGGCCTC TTCAAAATCT ATCCCCTCCC AGAAGACCCG
    GCTGTTCCCA TGCCCCCGAG ACAATTCCAC CAGCTGGCTG CCCAAGGGCC TCAGGAGTGC
    CTGGTCCGCA TCTATATCAT CCGAGCATTT GGCCTTCAGC CCAAGGACCC CAATGGGAAG
    TGTGATCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC
    ATTCCCTGTA CTCTGGAGCC TGTGTTTGGA AAGATGTTCG AGCTGACCTG CACTCTGCCT
    CTGGAGAAGG ACCTGAAGAT CACACTCTAT GACTATGACC TCCTCTCCAA AGATGAAAAG
    ATTGGGGAGA CAGTCATTGA CCTGGAGGAT AGGCTGCTGT CCAAGTTTGG GGCTCGCTGC
    GGACTCCCAC AGACGTACTG TGTCTCTGGA CCCAACCAGT GGAGGGACCA GCTCCGCCCC
    TCCCAGCTTC TCCACCTCTT CTGCCAGCAG CGCAGACTCA AGGCTCCTGT GTACCTCACG
    GACCACGTGG TGTTCCAGGA TAAAGAATAC AGCATTGAAG AAATAGAGGC TGGCCGAGTC
    CCGAACCCAC ACCTGGGCCC AGTGGAAGAG CGTCTGGCCT TGCATGTCCT CCAGCAGCAG
    GGCCTGGTCC CTGAGCATGT GGAGTCCCGG CCTCTCTACA GCCCCCTGCA GCCAGACATT
    GAACAGGGGA AGCTGCAGAT GTGGATCGAC CTATTTCCCA AGGCCCTGGG GCGTCCCGGA
    CCTCCCTTCA ACATCACCCC GCGGAGAGCC AGAAGGTTTT TCCTGCGTTG TATCATCTGG
    AACACAAAGG ACGTGATCCT GGACGACCTC AGCATCACAG GGGAGAAGAT GAGTGATATT
    TATGTGAAGG GTTGGATGGT TGGCTTTGAA GAGCACAAGC AAAAGACAGA TGTGCATTAC
    CGGTCCCTGG GAGGTGAAGG CAACTTTAAC TGGAGGTTCA TTTTCCCCTT TGACTACCTG
    CCAGCCGAGC AAGTCTGCAC CGTCTCTAAG AAGGATGCTT TCTGGAGGCT GGACAAGACT
    GAGAGCAAAA TCCCAGCACG AGTGGTATTT CAGATCTGGG ACAATGACAA GTTCTCCTTT
    GACGACTTTC TGGGCTCCTT GCAGTTGGAT CTCAACCACA TGCCCAAGCC TGCCAAGACA
    GCTGAGAAGT GCTCCTTGGA CCAGCTGGAT GACACTTTCC ACCCGGAGTG GTTTGTGTCC
    CTTTTTGAAC AGAAAACAGT TAAGGGCTGG TGGCCCTGCG TAGCAGAAGA GGGCGAGAAG
    AAGATCCTGG CAGGCAAGCT GGAAATGACC TTGGAGATCG TAGCAGAGAG TGAGCATGAG
    GAGCGGCCTG CTGGCCAGGG CCGGGATGAA CCCAACATGA ACCCCAAGCT CGAGGACCCA
    AGGCGCCCTG ACACCTCCTT CCTCTGGTTC ACCTCTCCAT ACAAGACTAT GAAGTTCATC
    CTGTGGCGGC GGTTCCGATG TGCCATTATC CTCTTCATCA TTCTCTTCAT CCTGCTACTA
    TTCTTAGGCA TCTTCATCTA TGCTTTCCCG AACTACGCTG CCATGAAGCT GGTGAAGCCC
    TTCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003432282.1
    CDS51..6419
    Translation

    Target ORF information:

    RefSeq Version XM_003432234.3
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris dysferlin (DYSF), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003432234.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG
    CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC
    GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG
    AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT
    CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG
    ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT
    GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT
    TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC
    GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT
    ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT
    GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC
    GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC
    AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC
    AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC
    ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG
    GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT
    GCCCCTGGAG GAGAAATAGA AGTGGATGAC CATCTGGGCT TCCTTCCCAC CTTCGGGCCC
    TGCTTCGTCA ATCTCTATGG CAGCCCCAGG GAATTCACTG GCTTCCCGGA CCCCTACGCG
    GAACTCAACA TGGGCAAGGG GGAAGGCGTG GCCTACCGAG GTCGGCTTCT GCTCTCCCTG
    GAGACCAAGC TGGTGGAGCA CAGCGGCGAG CAAAGGGTGG AGGACCTCCC TGCTGATGAC
    ATCCTTCGGG TGGAGAAATA CCTCCGGAGG CGCAAGTACT CTCTCTTTGC CGCCTTCTAC
    TCGGCCACCA TGCTCCAGGA TGTCGACGAT GCCATCCAGT TTGAGGTCAG CATTGGGAAC
    TATGGCAACA AGTTCGACAC GACCTGTCTG CCACTGGCCT CCACCACCCA GTACAGCCGG
    GCAGTCTTTG ATGGGTGCCA CTACTACTAC TTGCCCTGGG GCAATGTGAA GCCTGTGGTG
    GTGCTGTCAT CCTACTGGGA AGACATCAGC CACAGAGTCG AGGCTCAGAA CCAGCTGCTC
    AGGATCGCTG ACCATCTGGA AGCTGGCCTG GAGCAAGTCC ACTTGGCTTT AAAGGCGCAG
    TTCAACACAG AGGATGTGGA CTCCCTGTTG GCCCAGCTGA TGGATGAGCT CATCTCAGAC
    TGCAGCCAGC CCCTGGGCAA TGTCCAAGAG ATACCCTCTG CCACCCACCT GGACCGGCAC
    CTGTACAGGC TGCGCACCCG TCACCTAGGC CAGATTACTG AGGCGGCCCT GGCTCTGAAA
    CTCGGGCATG GCAAGCTCCC TGCCGCCCTG GAGCAGGCTG AAGACTGGCT CCTGCGTCTC
    CGTGCCCTGG CTGATGAGCC CCAGAACAGC CTGCCCGACA TCATCATCTG GATGCTGCAG
    GGAAACAAGC GAGTGGCGTA CCAGCGGGTG CCTGCGCACG AGGTCCTCTT CTCCCGGCGG
    GGCGCCAGCT ACTGTGGCAA GAACTGTGGG AAGCTGCAGA CGATATTTCT GAAATATCCC
    ATGGAGGGGG TGCCCGGAGC CCGGATGCCA GTACAAATAC GGGTGAAACT CTGGTTTGGG
    CTTTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GAAAGCTGTC CGTCTTTGCT
    GAAACTTATG AGAACCAGAC CAAACTGGCC CTGGTTGGGA ACTGGGGCAC AACAGGCCTC
    ACCTACCCCA AGTTTTCTGA TGTCATGGGC AAGATCAAGC TACCCAAGGA TAGCTTCCGC
    CCCTCAGCTG GCTGGACCTG GGCCGGAGAC TGGTTCGTGT GTCCAGAGAA GACCCTACTC
    CATGACATGG ACGCGGGCCA CCTGAGTTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG
    CTCCCTGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CAGATGTGAA TGGGGATAAG
    GTGCTTCCTA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAAGAGTGG
    TCCACGGACC TCAATCGGGC CGTCGATGAG CAAGGCTGGG AGTACAGTAT CACCATCCCC
    CCGGATCGGA AGCCAAAGCA CTGGGTTCCT GCTGAGAAGA TGTATTACAC GCACCGTCGG
    CGGCGCTGGG TCCGCCTGCG CAGAAGGGAC CTCAGCCAGA TGGAGGCATT GAAGAGGCAC
    AGACAGGCAG AGGCAGAGGG TGAGGGCTGG GAGTACGCCT CGCTCTTGGG CTGGAAGTTC
    CACCTGGAGT ACCGCAAGAC TGATGCTTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAA
    CCGCTGGAGA AGACGGGGCC TGCAGCCGTG TTTGCCCTTG AGGGGGCTCT GGGTGGTGTG
    GTAGATGACA GGAGTGAAGA TTCCGTGTCC GTGTCCACCT TGAGCTTTGG AGTGAACAGA
    CCCACGATTT CCTGCATCTT TGATTATGGG AACCGCTACC ACCTACGCTG CTACATGTAC
    CAGGCTCGGG ACCTGCCCTC GATGGACAAG GACTCTTTTT CTGATCCCTA CGCCATCGTC
    TCCTTCCTGC ACCAGAGCCA GAAGACAGTG GTGGCGAAGA ACACTCTGAA CCCCACCTGG
    GACCAGACGC TTATCTTCTA TGAGATTGAG ATCTTTGGTG AGCCAGCCAG TATCGCTGAG
    CAGCCGCCCA GCATTGTGGT GGAGCTGTAT GACCACGACA CCTACGGTGC AGATGAGTTT
    ATGGGACGCT GCATTTGTCA GCCCAGCCTG GAGCGGACAC CCCGGCTGGC CTGGTTCCCA
    CTGATTAGGG GCAGCCAGTC GGCGGGGGAA CTGCTGGCCT CTTTTGAGCT CATCCAGAGA
    GAAAAGCCGG CCATTCACCA TATTCCTGGT TTTGAGGTAC AGGATATGTC AGGAATCCTG
    GAAGAGTCAG AGGACATAGA CCTGCCCTAC CCGCCACCTC AGAGGGAGGC CAACATCTTC
    ATGGTCCCCC AGAACATCAA GCCTGTTCTC CAGCGTACTG CCATAGAGAT CCTGGCGTGG
    GGCCTGCGGA ACATGAAGAG CTACCAGCTA GCCAGCATCT CCTCCCCTAG CCTTGTGGTA
    GAGTGCGGGG GCCAGACAGT GCAGTCCTGT GTCATCAAGA ACCTCCGGAA GAACCCCAAC
    TTCGACATCT GTACCCTTTT CATGGAAGTG ATGCTGCCCA AGGAGGAGGT CTACTGTCCC
    TCCATCGTGA TCAAGGTCAT TGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG
    TGTACCATCC GTTCCCTGGA GAGCTTCATG TGTGACCTGT ACTCCGAGGA GAATCCGTCC
    CCACAGGGCG GCCCAGATGA TGTGAGTTTA CTCAGTCCTG GGGAAGACGT GCTCATTGAC
    ATCGATGACA AGGAGCCCCT CATCCCCATG CAGCTTGCAG ATGGTCTGTC GGGCTCTGCC
    CCCACTAACA TGGCATCTTC TCCATCCAGT CCTCATGAGG AAGAGTTCAT CGACTGGTGG
    AGCAAATTCT TTGCCTCCAC AGGGGAGAGG GAAAAGTGCG GCTCATACTT GGAGAAGGGC
    TTTGACACTC TAAAGGTCTA TGACACACAA CTGGAGAACG TGGAGGCCTT TGAGGGCCTG
    TCTGACTTTT GTAACACCTT CAAGCTCTAC CGGGGCAAGA CTCAGGAGGA GACAGAGGAC
    CCATCTGTTA TTGGGGAGTT TAAGGGCCTC TTCAAAATCT ATCCCCTCCC AGAAGACCCG
    GCTGTTCCCA TGCCCCCGAG ACAATTCCAC CAGCTGGCTG CCCAAGGGCC TCAGGAGTGC
    CTGGTCCGCA TCTATATCAT CCGAGCATTT GGCCTTCAGC CCAAGGACCC CAATGGGAAG
    TGTGATCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC
    ATTCCCTGTA CTCTGGAGCC TGTGTTTGGA AAGATGTTCG AGCTGACCTG CACTCTGCCT
    CTGGAGAAGG ACCTGAAGAT CACACTCTAT GACTATGACC TCCTCTCCAA AGATGAAAAG
    ATTGGGGAGA CAGTCATTGA CCTGGAGGAT AGGCTGCTGT CCAAGTTTGG GGCTCGCTGC
    GGACTCCCAC AGACGTACTG TGTCTCTGGA CCCAACCAGT GGAGGGACCA GCTCCGCCCC
    TCCCAGCTTC TCCACCTCTT CTGCCAGCAG CGCAGACTCA AGGCTCCTGT GTACCTCACG
    GACCACGTGG TGTTCCAGGA TAAAGAATAC AGCATTGAAG AAATAGAGGC TGGCCGAGTC
    CCGAACCCAC ACCTGGGCCC AGTGGAAGAG CGTCTGGCCT TGCATGTCCT CCAGCAGCAG
    GGCCTGGTCC CTGAGCATGT GGAGTCCCGG CCTCTCTACA GCCCCCTGCA GCCAGACATT
    GAACAGGGGA AGCTGCAGAT GTGGATCGAC CTATTTCCCA AGGCCCTGGG GCGTCCCGGA
    CCTCCCTTCA ACATCACCCC GCGGAGAGCC AGAAGGTTTT TCCTGCGTTG TATCATCTGG
    AACACAAAGG ACGTGATCCT GGACGACCTC AGCATCACAG GGGAGAAGAT GAGTGATATT
    TATGTGAAGG GTTGGATGGT TGGCTTTGAA GAGCACAAGC AAAAGACAGA TGTGCATTAC
    CGGTCCCTGG GAGGTGAAGG CAACTTTAAC TGGAGGTTCA TTTTCCCCTT TGACTACCTG
    CCAGCCGAGC AAGTCTGCAC CGTCTCTAAG AAGGATGCTT TCTGGAGGCT GGACAAGACT
    GAGAGCAAAA TCCCAGCACG AGTGGTATTT CAGATCTGGG ACAATGACAA GTTCTCCTTT
    GACGACTTTC TGGGCTCCTT GCAGTTGGAT CTCAACCACA TGCCCAAGCC TGCCAAGACA
    GCTGAGAAGT GCTCCTTGGA CCAGCTGGAT GACACTTTCC ACCCGGAGTG GTTTGTGTCC
    CTTTTTGAAC AGAAAACAGT TAAGGGCTGG TGGCCCTGCG TAGCAGAAGA GGGCGAGAAG
    AAGATCCTGG CAGGCAAGCT GGAAATGACC TTGGAGATCG TAGCAGAGAG TGAGCATGAG
    GAGCGGCCTG CTGGCCAGGG CCGGGATGAA CCCAACATGA ACCCCAAGCT CGAGGACCCA
    AGGCGCCCTG ACACCTCCTT CCTCTGGTTC ACCTCTCCAT ACAAGACTAT GAAGTTCATC
    CTGTGGCGGC GGTTCCGATG TGCCATTATC CTCTTCATCA TTCTCTTCAT CCTGCTACTA
    TTCTTAGGCA TCTTCATCTA TGCTTTCCCG AACTACGCTG CCATGAAGCT GGTGAAGCCC
    TTCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe19207
    Clone ID Related Accession (Same CDS sequence) XM_003432229.3 , XM_003432229.4
    Accession Version XM_003432229.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6366bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product dysferlin isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003432229.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC
    CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC
    TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA
    CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGGTG GGGGACAGAG CCGGGCTGAG
    ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GATACAAGAT ACTCTGGAAA GAAGTGGCTG
    GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC TGGAGACGAG
    GGAGAGCCAT TACTGGATCA GAGTGGCGTC CCAGGCCCTG GGGCTCCCAC CACCCCGAGG
    AAGCCACCTT CCCATCTGCC CCCCTACCAC CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC
    GCAGCCAGAA AGCTGCTGTC CGACAAACCG CAGGACTTCC AGATCAGAGT GCAAGTGATC
    GAGGGCCGCC AGCTGCCAGG GGTGAATATC AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG
    CAGACCAAGA GGACACGGAT CCACAAGGGA AACAGCCCAC TATTTAACGA GACTCTTTTC
    TTCAATGTGT TTGACTCTCC TTCGGAGCTG TTTGATGAGC CCATCTTTAT CACGGTCGTA
    GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC
    ATCTACAGAG AGCCCCGACA TGCTTATCTG AGGAAGTGGC TGCTACTCTC CAACCCTGAT
    GACTTCTCTG TTGGGGCCAG AGGTTACCTG AAAGCAAGCC TTTATGTGCT GGGGCCCGGA
    GATGAAGCGC CTCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT TGAAAACAAT
    CTGCTCAGGC CAACTGGCGT GGCCCTGCGA GGAGCCCACT TCTGCCTGAA GATCTTCAGA
    GCGGAGGACT TACCACAAAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC
    TTTGACAGCA ACAAGAAGAA TTTGGTAGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA
    ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACT
    CTGCCTGCCA TGTTTCCCTC CATGTGTGAA AAAATGAAGA TTCGCGTCAT GGACTGGGAC
    CGCCTTACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC
    CCTGGAGGAG AAATAGAAGT GGATGACCAT CTGGGCTTCC TTCCCACCTT CGGGCCCTGC
    TTCGTCAATC TCTATGGCAG CCCCAGGGAA TTCACTGGCT TCCCGGACCC CTACGCGGAA
    CTCAACATGG GCAAGGGGGA AGGCGTGGCC TACCGAGGTC GGCTTCTGCT CTCCCTGGAG
    ACCAAGCTGG TGGAGCACAG CGGCGAGCAA AGGGTGGAGG ACCTCCCTGC TGATGACATC
    CTTCGGGTGG AGAAATACCT CCGGAGGCGC AAGTACTCTC TCTTTGCCGC CTTCTACTCG
    GCCACCATGC TCCAGGATGT CGACGATGCC ATCCAGTTTG AGGTCAGCAT TGGGAACTAT
    GGCAACAAGT TCGACACGAC CTGTCTGCCA CTGGCCTCCA CCACCCAGTA CAGCCGGGCA
    GTCTTTGATG GGTGCCACTA CTACTACTTG CCCTGGGGCA ATGTGAAGCC TGTGGTGGTG
    CTGTCATCCT ACTGGGAAGA CATCAGCCAC AGAGTCGAGG CTCAGAACCA GCTGCTCAGG
    ATCGCTGACC ATCTGGAAGC TGGCCTGGAG CAAGTCCACT TGGCTTTAAA GGCGCAGTTC
    AACACAGAGG ATGTGGACTC CCTGTTGGCC CAGCTGATGG ATGAGCTCAT CTCAGACTGC
    AGCCAGCCCC TGGGCAATGT CCAAGAGATA CCCTCTGCCA CCCACCTGGA CCGGCACCTG
    TACAGGCTGC GCACCCGTCA CCTAGGCCAG ATTACTGAGG CGGCCCTGGC TCTGAAACTC
    GGGCATGGCA AGCTCCCTGC CGCCCTGGAG CAGGCTGAAG ACTGGCTCCT GCGTCTCCGT
    GCCCTGGCTG ATGAGCCCCA GAACAGCCTG CCCGACATCA TCATCTGGAT GCTGCAGGGA
    AACAAGCGAG TGGCGTACCA GCGGGTGCCT GCGCACGAGG TCCTCTTCTC CCGGCGGGGC
    GCCAGCTACT GTGGCAAGAA CTGTGGGAAG CTGCAGACGA TATTTCTGAA ATATCCCATG
    GAGGGGGTGC CCGGAGCCCG GATGCCAGTA CAAATACGGG TGAAACTCTG GTTTGGGCTT
    TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGAA AGCTGTCCGT CTTTGCTGAA
    ACTTATGAGA ACCAGACCAA ACTGGCCCTG GTTGGGAACT GGGGCACAAC AGGCCTCACC
    TACCCCAAGT TTTCTGATGT CATGGGCAAG ATCAAGCTAC CCAAGGATAG CTTCCGCCCC
    TCAGCTGGCT GGACCTGGGC CGGAGACTGG TTCGTGTGTC CAGAGAAGAC CCTACTCCAT
    GACATGGACG CGGGCCACCT GAGTTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTC
    CCTGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACAG ATGTGAATGG GGATAAGGTG
    CTTCCTAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA AGAGTGGTCC
    ACGGACCTCA ATCGGGCCGT CGATGAGCAA GGCTGGGAGT ACAGTATCAC CATCCCCCCG
    GATCGGAAGC CAAAGCACTG GGTTCCTGCT GAGAAGATGT ATTACACGCA CCGTCGGCGG
    CGCTGGGTCC GCCTGCGCAG AAGGGACCTC AGCCAGATGG AGGCATTGAA GAGGCACAGA
    CAGGCAGAGG CAGAGGGTGA GGGCTGGGAG TACGCCTCGC TCTTGGGCTG GAAGTTCCAC
    CTGGAGTACC GCAAGACTGA TGCTTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAACCG
    CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT GCCCTTGAGG GGGCTCTGGG TGGTGTGGTA
    GATGACAGGA GTGAAGATTC CGTGTCCGTG TCCACCTTGA GCTTTGGAGT GAACAGACCC
    ACGATTTCCT GCATCTTTGA TTATGGGAAC CGCTACCACC TACGCTGCTA CATGTACCAG
    GCTCGGGACC TGCCCTCGAT GGACAAGGAC TCTTTTTCTG ATCCCTACGC CATCGTCTCC
    TTCCTGCACC AGAGCCAGAA GACAGTGGTG GCGAAGAACA CTCTGAACCC CACCTGGGAC
    CAGACGCTTA TCTTCTATGA GATTGAGATC TTTGGTGAGC CAGCCAGTAT CGCTGAGCAG
    CCGCCCAGCA TTGTGGTGGA GCTGTATGAC CACGACACCT ACGGTGCAGA TGAGTTTATG
    GGACGCTGCA TTTGTCAGCC CAGCCTGGAG CGGACACCCC GGCTGGCCTG GTTCCCACTG
    ATTAGGGGCA GCCAGTCGGC GGGGGAACTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAA
    AAGCCGGCCA TTCACCATAT TCCTGGTTTT GAGGTACAGG ATATGTCAGG AATCCTGGAA
    GAGTCAGAGG ACATAGACCT GCCCTACCCG CCACCTCAGA GGGAGGCCAA CATCTTCATG
    GTCCCCCAGA ACATCAAGCC TGTTCTCCAG CGTACTGCCA TAGAGATCCT GGCGTGGGGC
    CTGCGGAACA TGAAGAGCTA CCAGCTAGCC AGCATCTCCT CCCCTAGCCT TGTGGTAGAG
    TGCGGGGGCC AGACAGTGCA GTCCTGTGTC ATCAAGAACC TCCGGAAGAA CCCCAACTTC
    GACATCTGTA CCCTTTTCAT GGAAGTGATG CTGCCCAAGG AGGAGGTCTA CTGTCCCTCC
    ATCGTGATCA AGGTCATTGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT
    ACCATCCGTT CCCTGGAGAG CTTCATGTGT GACCTGTACT CCGAGGAGAA TCCGTCCCCA
    CAGGGCGGCC CAGATGATGT GAGTTTACTC AGTCCTGGGG AAGACGTGCT CATTGACATC
    GATGACAAGG AGCCCCTCAT CCCCATGCAG CTTGCAGATG GTCTGTCGGG CTCTGCCCCC
    ACTAACATGG CATCTTCTCC ATCCAGTCCT CATGAGGAAG AGTTCATCGA CTGGTGGAGC
    AAATTCTTTG CCTCCACAGG GGAGAGGGAA AAGTGCGGCT CATACTTGGA GAAGGGCTTT
    GACACTCTAA AGGTCTATGA CACACAACTG GAGAACGTGG AGGCCTTTGA GGGCCTGTCT
    GACTTTTGTA ACACCTTCAA GCTCTACCGG GGCAAGACTC AGGAGGAGAC AGAGGACCCA
    TCTGTTATTG GGGAGTTTAA GGGCCTCTTC AAAATCTATC CCCTCCCAGA AGACCCGGCT
    GTTCCCATGC CCCCGAGACA ATTCCACCAG CTGGCTGCCC AAGGGCCTCA GGAGTGCCTG
    GTCCGCATCT ATATCATCCG AGCATTTGGC CTTCAGCCCA AGGACCCCAA TGGGAAGTGT
    GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATT
    CCCTGTACTC TGGAGCCTGT GTTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG
    GAGAAGGACC TGAAGATCAC ACTCTATGAC TATGACCTCC TCTCCAAAGA TGAAAAGATT
    GGGGAGACAG TCATTGACCT GGAGGATAGG CTGCTGTCCA AGTTTGGGGC TCGCTGCGGA
    CTCCCACAGA CGTACTGTGT CTCTGGACCC AACCAGTGGA GGGACCAGCT CCGCCCCTCC
    CAGCTTCTCC ACCTCTTCTG CCAGCAGCGC AGACTCAAGG CTCCTGTGTA CCTCACGGAC
    CACGTGGTGT TCCAGGATAA AGAATACAGC ATTGAAGAAA TAGAGGCTGG CCGAGTCCCG
    AACCCACACC TGGGCCCAGT GGAAGAGCGT CTGGCCTTGC ATGTCCTCCA GCAGCAGGGC
    CTGGTCCCTG AGCATGTGGA GTCCCGGCCT CTCTACAGCC CCCTGCAGCC AGACATTGAA
    CAGGGGAAGC TGCAGATGTG GATCGACCTA TTTCCCAAGG CCCTGGGGCG TCCCGGACCT
    CCCTTCAACA TCACCCCGCG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT CATCTGGAAC
    ACAAAGGACG TGATCCTGGA CGACCTCAGC ATCACAGGGG AGAAGATGAG TGATATTTAT
    GTGAAGGGTT GGATGGTTGG CTTTGAAGAG CACAAGCAAA AGACAGATGT GCATTACCGG
    TCCCTGGGAG GTGAAGGCAA CTTTAACTGG AGGTTCATTT TCCCCTTTGA CTACCTGCCA
    GCCGAGCAAG TCTGCACCGT CTCTAAGAAG GATGCTTTCT GGAGGCTGGA CAAGACTGAG
    AGCAAAATCC CAGCACGAGT GGTATTTCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAC
    GACTTTCTGG GCTCCTTGCA GTTGGATCTC AACCACATGC CCAAGCCTGC CAAGACAGCT
    GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACTTTCCACC CGGAGTGGTT TGTGTCCCTT
    TTTGAACAGA AAACAGTTAA GGGCTGGTGG CCCTGCGTAG CAGAAGAGGG CGAGAAGAAG
    ATCCTGGCAG GCAAGCTGGA AATGACCTTG GAGATCGTAG CAGAGAGTGA GCATGAGGAG
    CGGCCTGCTG GCCAGGGCCG GGATGAACCC AACATGAACC CCAAGCTCGA GGACCCAAGG
    CGCCCTGACA CCTCCTTCCT CTGGTTCACC TCTCCATACA AGACTATGAA GTTCATCCTG
    TGGCGGCGGT TCCGATGTGC CATTATCCTC TTCATCATTC TCTTCATCCT GCTACTATTC
    TTAGGCATCT TCATCTATGC TTTCCCGAAC TACGCTGCCA TGAAGCTGGT GAAGCCCTTC
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003432277.1
    CDS439..6804
    Translation

    Target ORF information:

    RefSeq Version XM_003432229.3
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris dysferlin (DYSF), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003432229.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC
    CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC
    TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA
    CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGGTG GGGGACAGAG CCGGGCTGAG
    ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GATACAAGAT ACTCTGGAAA GAAGTGGCTG
    GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC TGGAGACGAG
    GGAGAGCCAT TACTGGATCA GAGTGGCGTC CCAGGCCCTG GGGCTCCCAC CACCCCGAGG
    AAGCCACCTT CCCATCTGCC CCCCTACCAC CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC
    GCAGCCAGAA AGCTGCTGTC CGACAAACCG CAGGACTTCC AGATCAGAGT GCAAGTGATC
    GAGGGCCGCC AGCTGCCAGG GGTGAATATC AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG
    CAGACCAAGA GGACACGGAT CCACAAGGGA AACAGCCCAC TATTTAACGA GACTCTTTTC
    TTCAATGTGT TTGACTCTCC TTCGGAGCTG TTTGATGAGC CCATCTTTAT CACGGTCGTA
    GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC
    ATCTACAGAG AGCCCCGACA TGCTTATCTG AGGAAGTGGC TGCTACTCTC CAACCCTGAT
    GACTTCTCTG TTGGGGCCAG AGGTTACCTG AAAGCAAGCC TTTATGTGCT GGGGCCCGGA
    GATGAAGCGC CTCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT TGAAAACAAT
    CTGCTCAGGC CAACTGGCGT GGCCCTGCGA GGAGCCCACT TCTGCCTGAA GATCTTCAGA
    GCGGAGGACT TACCACAAAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC
    TTTGACAGCA ACAAGAAGAA TTTGGTAGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA
    ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACT
    CTGCCTGCCA TGTTTCCCTC CATGTGTGAA AAAATGAAGA TTCGCGTCAT GGACTGGGAC
    CGCCTTACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC
    CCTGGAGGAG AAATAGAAGT GGATGACCAT CTGGGCTTCC TTCCCACCTT CGGGCCCTGC
    TTCGTCAATC TCTATGGCAG CCCCAGGGAA TTCACTGGCT TCCCGGACCC CTACGCGGAA
    CTCAACATGG GCAAGGGGGA AGGCGTGGCC TACCGAGGTC GGCTTCTGCT CTCCCTGGAG
    ACCAAGCTGG TGGAGCACAG CGGCGAGCAA AGGGTGGAGG ACCTCCCTGC TGATGACATC
    CTTCGGGTGG AGAAATACCT CCGGAGGCGC AAGTACTCTC TCTTTGCCGC CTTCTACTCG
    GCCACCATGC TCCAGGATGT CGACGATGCC ATCCAGTTTG AGGTCAGCAT TGGGAACTAT
    GGCAACAAGT TCGACACGAC CTGTCTGCCA CTGGCCTCCA CCACCCAGTA CAGCCGGGCA
    GTCTTTGATG GGTGCCACTA CTACTACTTG CCCTGGGGCA ATGTGAAGCC TGTGGTGGTG
    CTGTCATCCT ACTGGGAAGA CATCAGCCAC AGAGTCGAGG CTCAGAACCA GCTGCTCAGG
    ATCGCTGACC ATCTGGAAGC TGGCCTGGAG CAAGTCCACT TGGCTTTAAA GGCGCAGTTC
    AACACAGAGG ATGTGGACTC CCTGTTGGCC CAGCTGATGG ATGAGCTCAT CTCAGACTGC
    AGCCAGCCCC TGGGCAATGT CCAAGAGATA CCCTCTGCCA CCCACCTGGA CCGGCACCTG
    TACAGGCTGC GCACCCGTCA CCTAGGCCAG ATTACTGAGG CGGCCCTGGC TCTGAAACTC
    GGGCATGGCA AGCTCCCTGC CGCCCTGGAG CAGGCTGAAG ACTGGCTCCT GCGTCTCCGT
    GCCCTGGCTG ATGAGCCCCA GAACAGCCTG CCCGACATCA TCATCTGGAT GCTGCAGGGA
    AACAAGCGAG TGGCGTACCA GCGGGTGCCT GCGCACGAGG TCCTCTTCTC CCGGCGGGGC
    GCCAGCTACT GTGGCAAGAA CTGTGGGAAG CTGCAGACGA TATTTCTGAA ATATCCCATG
    GAGGGGGTGC CCGGAGCCCG GATGCCAGTA CAAATACGGG TGAAACTCTG GTTTGGGCTT
    TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGAA AGCTGTCCGT CTTTGCTGAA
    ACTTATGAGA ACCAGACCAA ACTGGCCCTG GTTGGGAACT GGGGCACAAC AGGCCTCACC
    TACCCCAAGT TTTCTGATGT CATGGGCAAG ATCAAGCTAC CCAAGGATAG CTTCCGCCCC
    TCAGCTGGCT GGACCTGGGC CGGAGACTGG TTCGTGTGTC CAGAGAAGAC CCTACTCCAT
    GACATGGACG CGGGCCACCT GAGTTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTC
    CCTGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACAG ATGTGAATGG GGATAAGGTG
    CTTCCTAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA AGAGTGGTCC
    ACGGACCTCA ATCGGGCCGT CGATGAGCAA GGCTGGGAGT ACAGTATCAC CATCCCCCCG
    GATCGGAAGC CAAAGCACTG GGTTCCTGCT GAGAAGATGT ATTACACGCA CCGTCGGCGG
    CGCTGGGTCC GCCTGCGCAG AAGGGACCTC AGCCAGATGG AGGCATTGAA GAGGCACAGA
    CAGGCAGAGG CAGAGGGTGA GGGCTGGGAG TACGCCTCGC TCTTGGGCTG GAAGTTCCAC
    CTGGAGTACC GCAAGACTGA TGCTTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAACCG
    CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT GCCCTTGAGG GGGCTCTGGG TGGTGTGGTA
    GATGACAGGA GTGAAGATTC CGTGTCCGTG TCCACCTTGA GCTTTGGAGT GAACAGACCC
    ACGATTTCCT GCATCTTTGA TTATGGGAAC CGCTACCACC TACGCTGCTA CATGTACCAG
    GCTCGGGACC TGCCCTCGAT GGACAAGGAC TCTTTTTCTG ATCCCTACGC CATCGTCTCC
    TTCCTGCACC AGAGCCAGAA GACAGTGGTG GCGAAGAACA CTCTGAACCC CACCTGGGAC
    CAGACGCTTA TCTTCTATGA GATTGAGATC TTTGGTGAGC CAGCCAGTAT CGCTGAGCAG
    CCGCCCAGCA TTGTGGTGGA GCTGTATGAC CACGACACCT ACGGTGCAGA TGAGTTTATG
    GGACGCTGCA TTTGTCAGCC CAGCCTGGAG CGGACACCCC GGCTGGCCTG GTTCCCACTG
    ATTAGGGGCA GCCAGTCGGC GGGGGAACTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAA
    AAGCCGGCCA TTCACCATAT TCCTGGTTTT GAGGTACAGG ATATGTCAGG AATCCTGGAA
    GAGTCAGAGG ACATAGACCT GCCCTACCCG CCACCTCAGA GGGAGGCCAA CATCTTCATG
    GTCCCCCAGA ACATCAAGCC TGTTCTCCAG CGTACTGCCA TAGAGATCCT GGCGTGGGGC
    CTGCGGAACA TGAAGAGCTA CCAGCTAGCC AGCATCTCCT CCCCTAGCCT TGTGGTAGAG
    TGCGGGGGCC AGACAGTGCA GTCCTGTGTC ATCAAGAACC TCCGGAAGAA CCCCAACTTC
    GACATCTGTA CCCTTTTCAT GGAAGTGATG CTGCCCAAGG AGGAGGTCTA CTGTCCCTCC
    ATCGTGATCA AGGTCATTGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT
    ACCATCCGTT CCCTGGAGAG CTTCATGTGT GACCTGTACT CCGAGGAGAA TCCGTCCCCA
    CAGGGCGGCC CAGATGATGT GAGTTTACTC AGTCCTGGGG AAGACGTGCT CATTGACATC
    GATGACAAGG AGCCCCTCAT CCCCATGCAG CTTGCAGATG GTCTGTCGGG CTCTGCCCCC
    ACTAACATGG CATCTTCTCC ATCCAGTCCT CATGAGGAAG AGTTCATCGA CTGGTGGAGC
    AAATTCTTTG CCTCCACAGG GGAGAGGGAA AAGTGCGGCT CATACTTGGA GAAGGGCTTT
    GACACTCTAA AGGTCTATGA CACACAACTG GAGAACGTGG AGGCCTTTGA GGGCCTGTCT
    GACTTTTGTA ACACCTTCAA GCTCTACCGG GGCAAGACTC AGGAGGAGAC AGAGGACCCA
    TCTGTTATTG GGGAGTTTAA GGGCCTCTTC AAAATCTATC CCCTCCCAGA AGACCCGGCT
    GTTCCCATGC CCCCGAGACA ATTCCACCAG CTGGCTGCCC AAGGGCCTCA GGAGTGCCTG
    GTCCGCATCT ATATCATCCG AGCATTTGGC CTTCAGCCCA AGGACCCCAA TGGGAAGTGT
    GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATT
    CCCTGTACTC TGGAGCCTGT GTTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG
    GAGAAGGACC TGAAGATCAC ACTCTATGAC TATGACCTCC TCTCCAAAGA TGAAAAGATT
    GGGGAGACAG TCATTGACCT GGAGGATAGG CTGCTGTCCA AGTTTGGGGC TCGCTGCGGA
    CTCCCACAGA CGTACTGTGT CTCTGGACCC AACCAGTGGA GGGACCAGCT CCGCCCCTCC
    CAGCTTCTCC ACCTCTTCTG CCAGCAGCGC AGACTCAAGG CTCCTGTGTA CCTCACGGAC
    CACGTGGTGT TCCAGGATAA AGAATACAGC ATTGAAGAAA TAGAGGCTGG CCGAGTCCCG
    AACCCACACC TGGGCCCAGT GGAAGAGCGT CTGGCCTTGC ATGTCCTCCA GCAGCAGGGC
    CTGGTCCCTG AGCATGTGGA GTCCCGGCCT CTCTACAGCC CCCTGCAGCC AGACATTGAA
    CAGGGGAAGC TGCAGATGTG GATCGACCTA TTTCCCAAGG CCCTGGGGCG TCCCGGACCT
    CCCTTCAACA TCACCCCGCG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT CATCTGGAAC
    ACAAAGGACG TGATCCTGGA CGACCTCAGC ATCACAGGGG AGAAGATGAG TGATATTTAT
    GTGAAGGGTT GGATGGTTGG CTTTGAAGAG CACAAGCAAA AGACAGATGT GCATTACCGG
    TCCCTGGGAG GTGAAGGCAA CTTTAACTGG AGGTTCATTT TCCCCTTTGA CTACCTGCCA
    GCCGAGCAAG TCTGCACCGT CTCTAAGAAG GATGCTTTCT GGAGGCTGGA CAAGACTGAG
    AGCAAAATCC CAGCACGAGT GGTATTTCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAC
    GACTTTCTGG GCTCCTTGCA GTTGGATCTC AACCACATGC CCAAGCCTGC CAAGACAGCT
    GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACTTTCCACC CGGAGTGGTT TGTGTCCCTT
    TTTGAACAGA AAACAGTTAA GGGCTGGTGG CCCTGCGTAG CAGAAGAGGG CGAGAAGAAG
    ATCCTGGCAG GCAAGCTGGA AATGACCTTG GAGATCGTAG CAGAGAGTGA GCATGAGGAG
    CGGCCTGCTG GCCAGGGCCG GGATGAACCC AACATGAACC CCAAGCTCGA GGACCCAAGG
    CGCCCTGACA CCTCCTTCCT CTGGTTCACC TCTCCATACA AGACTATGAA GTTCATCCTG
    TGGCGGCGGT TCCGATGTGC CATTATCCTC TTCATCATTC TCTTCATCCT GCTACTATTC
    TTAGGCATCT TCATCTATGC TTTCCCGAAC TACGCTGCCA TGAAGCTGGT GAAGCCCTTC
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe19207
    Clone ID Related Accession (Same CDS sequence) XM_003432229.3 , XM_003432229.4
    Accession Version XM_003432229.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6366bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product dysferlin isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003432229.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC
    CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC
    TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA
    CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGGTG GGGGACAGAG CCGGGCTGAG
    ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GATACAAGAT ACTCTGGAAA GAAGTGGCTG
    GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC TGGAGACGAG
    GGAGAGCCAT TACTGGATCA GAGTGGCGTC CCAGGCCCTG GGGCTCCCAC CACCCCGAGG
    AAGCCACCTT CCCATCTGCC CCCCTACCAC CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC
    GCAGCCAGAA AGCTGCTGTC CGACAAACCG CAGGACTTCC AGATCAGAGT GCAAGTGATC
    GAGGGCCGCC AGCTGCCAGG GGTGAATATC AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG
    CAGACCAAGA GGACACGGAT CCACAAGGGA AACAGCCCAC TATTTAACGA GACTCTTTTC
    TTCAATGTGT TTGACTCTCC TTCGGAGCTG TTTGATGAGC CCATCTTTAT CACGGTCGTA
    GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC
    ATCTACAGAG AGCCCCGACA TGCTTATCTG AGGAAGTGGC TGCTACTCTC CAACCCTGAT
    GACTTCTCTG TTGGGGCCAG AGGTTACCTG AAAGCAAGCC TTTATGTGCT GGGGCCCGGA
    GATGAAGCGC CTCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT TGAAAACAAT
    CTGCTCAGGC CAACTGGCGT GGCCCTGCGA GGAGCCCACT TCTGCCTGAA GATCTTCAGA
    GCGGAGGACT TACCACAAAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC
    TTTGACAGCA ACAAGAAGAA TTTGGTAGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA
    ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACT
    CTGCCTGCCA TGTTTCCCTC CATGTGTGAA AAAATGAAGA TTCGCGTCAT GGACTGGGAC
    CGCCTTACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC
    CCTGGAGGAG AAATAGAAGT GGATGACCAT CTGGGCTTCC TTCCCACCTT CGGGCCCTGC
    TTCGTCAATC TCTATGGCAG CCCCAGGGAA TTCACTGGCT TCCCGGACCC CTACGCGGAA
    CTCAACATGG GCAAGGGGGA AGGCGTGGCC TACCGAGGTC GGCTTCTGCT CTCCCTGGAG
    ACCAAGCTGG TGGAGCACAG CGGCGAGCAA AGGGTGGAGG ACCTCCCTGC TGATGACATC
    CTTCGGGTGG AGAAATACCT CCGGAGGCGC AAGTACTCTC TCTTTGCCGC CTTCTACTCG
    GCCACCATGC TCCAGGATGT CGACGATGCC ATCCAGTTTG AGGTCAGCAT TGGGAACTAT
    GGCAACAAGT TCGACACGAC CTGTCTGCCA CTGGCCTCCA CCACCCAGTA CAGCCGGGCA
    GTCTTTGATG GGTGCCACTA CTACTACTTG CCCTGGGGCA ATGTGAAGCC TGTGGTGGTG
    CTGTCATCCT ACTGGGAAGA CATCAGCCAC AGAGTCGAGG CTCAGAACCA GCTGCTCAGG
    ATCGCTGACC ATCTGGAAGC TGGCCTGGAG CAAGTCCACT TGGCTTTAAA GGCGCAGTTC
    AACACAGAGG ATGTGGACTC CCTGTTGGCC CAGCTGATGG ATGAGCTCAT CTCAGACTGC
    AGCCAGCCCC TGGGCAATGT CCAAGAGATA CCCTCTGCCA CCCACCTGGA CCGGCACCTG
    TACAGGCTGC GCACCCGTCA CCTAGGCCAG ATTACTGAGG CGGCCCTGGC TCTGAAACTC
    GGGCATGGCA AGCTCCCTGC CGCCCTGGAG CAGGCTGAAG ACTGGCTCCT GCGTCTCCGT
    GCCCTGGCTG ATGAGCCCCA GAACAGCCTG CCCGACATCA TCATCTGGAT GCTGCAGGGA
    AACAAGCGAG TGGCGTACCA GCGGGTGCCT GCGCACGAGG TCCTCTTCTC CCGGCGGGGC
    GCCAGCTACT GTGGCAAGAA CTGTGGGAAG CTGCAGACGA TATTTCTGAA ATATCCCATG
    GAGGGGGTGC CCGGAGCCCG GATGCCAGTA CAAATACGGG TGAAACTCTG GTTTGGGCTT
    TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGAA AGCTGTCCGT CTTTGCTGAA
    ACTTATGAGA ACCAGACCAA ACTGGCCCTG GTTGGGAACT GGGGCACAAC AGGCCTCACC
    TACCCCAAGT TTTCTGATGT CATGGGCAAG ATCAAGCTAC CCAAGGATAG CTTCCGCCCC
    TCAGCTGGCT GGACCTGGGC CGGAGACTGG TTCGTGTGTC CAGAGAAGAC CCTACTCCAT
    GACATGGACG CGGGCCACCT GAGTTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTC
    CCTGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACAG ATGTGAATGG GGATAAGGTG
    CTTCCTAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA AGAGTGGTCC
    ACGGACCTCA ATCGGGCCGT CGATGAGCAA GGCTGGGAGT ACAGTATCAC CATCCCCCCG
    GATCGGAAGC CAAAGCACTG GGTTCCTGCT GAGAAGATGT ATTACACGCA CCGTCGGCGG
    CGCTGGGTCC GCCTGCGCAG AAGGGACCTC AGCCAGATGG AGGCATTGAA GAGGCACAGA
    CAGGCAGAGG CAGAGGGTGA GGGCTGGGAG TACGCCTCGC TCTTGGGCTG GAAGTTCCAC
    CTGGAGTACC GCAAGACTGA TGCTTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAACCG
    CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT GCCCTTGAGG GGGCTCTGGG TGGTGTGGTA
    GATGACAGGA GTGAAGATTC CGTGTCCGTG TCCACCTTGA GCTTTGGAGT GAACAGACCC
    ACGATTTCCT GCATCTTTGA TTATGGGAAC CGCTACCACC TACGCTGCTA CATGTACCAG
    GCTCGGGACC TGCCCTCGAT GGACAAGGAC TCTTTTTCTG ATCCCTACGC CATCGTCTCC
    TTCCTGCACC AGAGCCAGAA GACAGTGGTG GCGAAGAACA CTCTGAACCC CACCTGGGAC
    CAGACGCTTA TCTTCTATGA GATTGAGATC TTTGGTGAGC CAGCCAGTAT CGCTGAGCAG
    CCGCCCAGCA TTGTGGTGGA GCTGTATGAC CACGACACCT ACGGTGCAGA TGAGTTTATG
    GGACGCTGCA TTTGTCAGCC CAGCCTGGAG CGGACACCCC GGCTGGCCTG GTTCCCACTG
    ATTAGGGGCA GCCAGTCGGC GGGGGAACTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAA
    AAGCCGGCCA TTCACCATAT TCCTGGTTTT GAGGTACAGG ATATGTCAGG AATCCTGGAA
    GAGTCAGAGG ACATAGACCT GCCCTACCCG CCACCTCAGA GGGAGGCCAA CATCTTCATG
    GTCCCCCAGA ACATCAAGCC TGTTCTCCAG CGTACTGCCA TAGAGATCCT GGCGTGGGGC
    CTGCGGAACA TGAAGAGCTA CCAGCTAGCC AGCATCTCCT CCCCTAGCCT TGTGGTAGAG
    TGCGGGGGCC AGACAGTGCA GTCCTGTGTC ATCAAGAACC TCCGGAAGAA CCCCAACTTC
    GACATCTGTA CCCTTTTCAT GGAAGTGATG CTGCCCAAGG AGGAGGTCTA CTGTCCCTCC
    ATCGTGATCA AGGTCATTGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT
    ACCATCCGTT CCCTGGAGAG CTTCATGTGT GACCTGTACT CCGAGGAGAA TCCGTCCCCA
    CAGGGCGGCC CAGATGATGT GAGTTTACTC AGTCCTGGGG AAGACGTGCT CATTGACATC
    GATGACAAGG AGCCCCTCAT CCCCATGCAG CTTGCAGATG GTCTGTCGGG CTCTGCCCCC
    ACTAACATGG CATCTTCTCC ATCCAGTCCT CATGAGGAAG AGTTCATCGA CTGGTGGAGC
    AAATTCTTTG CCTCCACAGG GGAGAGGGAA AAGTGCGGCT CATACTTGGA GAAGGGCTTT
    GACACTCTAA AGGTCTATGA CACACAACTG GAGAACGTGG AGGCCTTTGA GGGCCTGTCT
    GACTTTTGTA ACACCTTCAA GCTCTACCGG GGCAAGACTC AGGAGGAGAC AGAGGACCCA
    TCTGTTATTG GGGAGTTTAA GGGCCTCTTC AAAATCTATC CCCTCCCAGA AGACCCGGCT
    GTTCCCATGC CCCCGAGACA ATTCCACCAG CTGGCTGCCC AAGGGCCTCA GGAGTGCCTG
    GTCCGCATCT ATATCATCCG AGCATTTGGC CTTCAGCCCA AGGACCCCAA TGGGAAGTGT
    GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATT
    CCCTGTACTC TGGAGCCTGT GTTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG
    GAGAAGGACC TGAAGATCAC ACTCTATGAC TATGACCTCC TCTCCAAAGA TGAAAAGATT
    GGGGAGACAG TCATTGACCT GGAGGATAGG CTGCTGTCCA AGTTTGGGGC TCGCTGCGGA
    CTCCCACAGA CGTACTGTGT CTCTGGACCC AACCAGTGGA GGGACCAGCT CCGCCCCTCC
    CAGCTTCTCC ACCTCTTCTG CCAGCAGCGC AGACTCAAGG CTCCTGTGTA CCTCACGGAC
    CACGTGGTGT TCCAGGATAA AGAATACAGC ATTGAAGAAA TAGAGGCTGG CCGAGTCCCG
    AACCCACACC TGGGCCCAGT GGAAGAGCGT CTGGCCTTGC ATGTCCTCCA GCAGCAGGGC
    CTGGTCCCTG AGCATGTGGA GTCCCGGCCT CTCTACAGCC CCCTGCAGCC AGACATTGAA
    CAGGGGAAGC TGCAGATGTG GATCGACCTA TTTCCCAAGG CCCTGGGGCG TCCCGGACCT
    CCCTTCAACA TCACCCCGCG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT CATCTGGAAC
    ACAAAGGACG TGATCCTGGA CGACCTCAGC ATCACAGGGG AGAAGATGAG TGATATTTAT
    GTGAAGGGTT GGATGGTTGG CTTTGAAGAG CACAAGCAAA AGACAGATGT GCATTACCGG
    TCCCTGGGAG GTGAAGGCAA CTTTAACTGG AGGTTCATTT TCCCCTTTGA CTACCTGCCA
    GCCGAGCAAG TCTGCACCGT CTCTAAGAAG GATGCTTTCT GGAGGCTGGA CAAGACTGAG
    AGCAAAATCC CAGCACGAGT GGTATTTCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAC
    GACTTTCTGG GCTCCTTGCA GTTGGATCTC AACCACATGC CCAAGCCTGC CAAGACAGCT
    GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACTTTCCACC CGGAGTGGTT TGTGTCCCTT
    TTTGAACAGA AAACAGTTAA GGGCTGGTGG CCCTGCGTAG CAGAAGAGGG CGAGAAGAAG
    ATCCTGGCAG GCAAGCTGGA AATGACCTTG GAGATCGTAG CAGAGAGTGA GCATGAGGAG
    CGGCCTGCTG GCCAGGGCCG GGATGAACCC AACATGAACC CCAAGCTCGA GGACCCAAGG
    CGCCCTGACA CCTCCTTCCT CTGGTTCACC TCTCCATACA AGACTATGAA GTTCATCCTG
    TGGCGGCGGT TCCGATGTGC CATTATCCTC TTCATCATTC TCTTCATCCT GCTACTATTC
    TTAGGCATCT TCATCTATGC TTTCCCGAAC TACGCTGCCA TGAAGCTGGT GAAGCCCTTC
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003432277.1
    CDS405..6770
    Translation

    Target ORF information:

    RefSeq Version XM_003432229.4
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris dysferlin (DYSF), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003432229.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC
    CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC
    TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA
    CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGGTG GGGGACAGAG CCGGGCTGAG
    ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GATACAAGAT ACTCTGGAAA GAAGTGGCTG
    GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC TGGAGACGAG
    GGAGAGCCAT TACTGGATCA GAGTGGCGTC CCAGGCCCTG GGGCTCCCAC CACCCCGAGG
    AAGCCACCTT CCCATCTGCC CCCCTACCAC CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC
    GCAGCCAGAA AGCTGCTGTC CGACAAACCG CAGGACTTCC AGATCAGAGT GCAAGTGATC
    GAGGGCCGCC AGCTGCCAGG GGTGAATATC AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG
    CAGACCAAGA GGACACGGAT CCACAAGGGA AACAGCCCAC TATTTAACGA GACTCTTTTC
    TTCAATGTGT TTGACTCTCC TTCGGAGCTG TTTGATGAGC CCATCTTTAT CACGGTCGTA
    GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC
    ATCTACAGAG AGCCCCGACA TGCTTATCTG AGGAAGTGGC TGCTACTCTC CAACCCTGAT
    GACTTCTCTG TTGGGGCCAG AGGTTACCTG AAAGCAAGCC TTTATGTGCT GGGGCCCGGA
    GATGAAGCGC CTCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT TGAAAACAAT
    CTGCTCAGGC CAACTGGCGT GGCCCTGCGA GGAGCCCACT TCTGCCTGAA GATCTTCAGA
    GCGGAGGACT TACCACAAAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC
    TTTGACAGCA ACAAGAAGAA TTTGGTAGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA
    ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACT
    CTGCCTGCCA TGTTTCCCTC CATGTGTGAA AAAATGAAGA TTCGCGTCAT GGACTGGGAC
    CGCCTTACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC
    CCTGGAGGAG AAATAGAAGT GGATGACCAT CTGGGCTTCC TTCCCACCTT CGGGCCCTGC
    TTCGTCAATC TCTATGGCAG CCCCAGGGAA TTCACTGGCT TCCCGGACCC CTACGCGGAA
    CTCAACATGG GCAAGGGGGA AGGCGTGGCC TACCGAGGTC GGCTTCTGCT CTCCCTGGAG
    ACCAAGCTGG TGGAGCACAG CGGCGAGCAA AGGGTGGAGG ACCTCCCTGC TGATGACATC
    CTTCGGGTGG AGAAATACCT CCGGAGGCGC AAGTACTCTC TCTTTGCCGC CTTCTACTCG
    GCCACCATGC TCCAGGATGT CGACGATGCC ATCCAGTTTG AGGTCAGCAT TGGGAACTAT
    GGCAACAAGT TCGACACGAC CTGTCTGCCA CTGGCCTCCA CCACCCAGTA CAGCCGGGCA
    GTCTTTGATG GGTGCCACTA CTACTACTTG CCCTGGGGCA ATGTGAAGCC TGTGGTGGTG
    CTGTCATCCT ACTGGGAAGA CATCAGCCAC AGAGTCGAGG CTCAGAACCA GCTGCTCAGG
    ATCGCTGACC ATCTGGAAGC TGGCCTGGAG CAAGTCCACT TGGCTTTAAA GGCGCAGTTC
    AACACAGAGG ATGTGGACTC CCTGTTGGCC CAGCTGATGG ATGAGCTCAT CTCAGACTGC
    AGCCAGCCCC TGGGCAATGT CCAAGAGATA CCCTCTGCCA CCCACCTGGA CCGGCACCTG
    TACAGGCTGC GCACCCGTCA CCTAGGCCAG ATTACTGAGG CGGCCCTGGC TCTGAAACTC
    GGGCATGGCA AGCTCCCTGC CGCCCTGGAG CAGGCTGAAG ACTGGCTCCT GCGTCTCCGT
    GCCCTGGCTG ATGAGCCCCA GAACAGCCTG CCCGACATCA TCATCTGGAT GCTGCAGGGA
    AACAAGCGAG TGGCGTACCA GCGGGTGCCT GCGCACGAGG TCCTCTTCTC CCGGCGGGGC
    GCCAGCTACT GTGGCAAGAA CTGTGGGAAG CTGCAGACGA TATTTCTGAA ATATCCCATG
    GAGGGGGTGC CCGGAGCCCG GATGCCAGTA CAAATACGGG TGAAACTCTG GTTTGGGCTT
    TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGAA AGCTGTCCGT CTTTGCTGAA
    ACTTATGAGA ACCAGACCAA ACTGGCCCTG GTTGGGAACT GGGGCACAAC AGGCCTCACC
    TACCCCAAGT TTTCTGATGT CATGGGCAAG ATCAAGCTAC CCAAGGATAG CTTCCGCCCC
    TCAGCTGGCT GGACCTGGGC CGGAGACTGG TTCGTGTGTC CAGAGAAGAC CCTACTCCAT
    GACATGGACG CGGGCCACCT GAGTTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTC
    CCTGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACAG ATGTGAATGG GGATAAGGTG
    CTTCCTAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA AGAGTGGTCC
    ACGGACCTCA ATCGGGCCGT CGATGAGCAA GGCTGGGAGT ACAGTATCAC CATCCCCCCG
    GATCGGAAGC CAAAGCACTG GGTTCCTGCT GAGAAGATGT ATTACACGCA CCGTCGGCGG
    CGCTGGGTCC GCCTGCGCAG AAGGGACCTC AGCCAGATGG AGGCATTGAA GAGGCACAGA
    CAGGCAGAGG CAGAGGGTGA GGGCTGGGAG TACGCCTCGC TCTTGGGCTG GAAGTTCCAC
    CTGGAGTACC GCAAGACTGA TGCTTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAACCG
    CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT GCCCTTGAGG GGGCTCTGGG TGGTGTGGTA
    GATGACAGGA GTGAAGATTC CGTGTCCGTG TCCACCTTGA GCTTTGGAGT GAACAGACCC
    ACGATTTCCT GCATCTTTGA TTATGGGAAC CGCTACCACC TACGCTGCTA CATGTACCAG
    GCTCGGGACC TGCCCTCGAT GGACAAGGAC TCTTTTTCTG ATCCCTACGC CATCGTCTCC
    TTCCTGCACC AGAGCCAGAA GACAGTGGTG GCGAAGAACA CTCTGAACCC CACCTGGGAC
    CAGACGCTTA TCTTCTATGA GATTGAGATC TTTGGTGAGC CAGCCAGTAT CGCTGAGCAG
    CCGCCCAGCA TTGTGGTGGA GCTGTATGAC CACGACACCT ACGGTGCAGA TGAGTTTATG
    GGACGCTGCA TTTGTCAGCC CAGCCTGGAG CGGACACCCC GGCTGGCCTG GTTCCCACTG
    ATTAGGGGCA GCCAGTCGGC GGGGGAACTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAA
    AAGCCGGCCA TTCACCATAT TCCTGGTTTT GAGGTACAGG ATATGTCAGG AATCCTGGAA
    GAGTCAGAGG ACATAGACCT GCCCTACCCG CCACCTCAGA GGGAGGCCAA CATCTTCATG
    GTCCCCCAGA ACATCAAGCC TGTTCTCCAG CGTACTGCCA TAGAGATCCT GGCGTGGGGC
    CTGCGGAACA TGAAGAGCTA CCAGCTAGCC AGCATCTCCT CCCCTAGCCT TGTGGTAGAG
    TGCGGGGGCC AGACAGTGCA GTCCTGTGTC ATCAAGAACC TCCGGAAGAA CCCCAACTTC
    GACATCTGTA CCCTTTTCAT GGAAGTGATG CTGCCCAAGG AGGAGGTCTA CTGTCCCTCC
    ATCGTGATCA AGGTCATTGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT
    ACCATCCGTT CCCTGGAGAG CTTCATGTGT GACCTGTACT CCGAGGAGAA TCCGTCCCCA
    CAGGGCGGCC CAGATGATGT GAGTTTACTC AGTCCTGGGG AAGACGTGCT CATTGACATC
    GATGACAAGG AGCCCCTCAT CCCCATGCAG CTTGCAGATG GTCTGTCGGG CTCTGCCCCC
    ACTAACATGG CATCTTCTCC ATCCAGTCCT CATGAGGAAG AGTTCATCGA CTGGTGGAGC
    AAATTCTTTG CCTCCACAGG GGAGAGGGAA AAGTGCGGCT CATACTTGGA GAAGGGCTTT
    GACACTCTAA AGGTCTATGA CACACAACTG GAGAACGTGG AGGCCTTTGA GGGCCTGTCT
    GACTTTTGTA ACACCTTCAA GCTCTACCGG GGCAAGACTC AGGAGGAGAC AGAGGACCCA
    TCTGTTATTG GGGAGTTTAA GGGCCTCTTC AAAATCTATC CCCTCCCAGA AGACCCGGCT
    GTTCCCATGC CCCCGAGACA ATTCCACCAG CTGGCTGCCC AAGGGCCTCA GGAGTGCCTG
    GTCCGCATCT ATATCATCCG AGCATTTGGC CTTCAGCCCA AGGACCCCAA TGGGAAGTGT
    GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATT
    CCCTGTACTC TGGAGCCTGT GTTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG
    GAGAAGGACC TGAAGATCAC ACTCTATGAC TATGACCTCC TCTCCAAAGA TGAAAAGATT
    GGGGAGACAG TCATTGACCT GGAGGATAGG CTGCTGTCCA AGTTTGGGGC TCGCTGCGGA
    CTCCCACAGA CGTACTGTGT CTCTGGACCC AACCAGTGGA GGGACCAGCT CCGCCCCTCC
    CAGCTTCTCC ACCTCTTCTG CCAGCAGCGC AGACTCAAGG CTCCTGTGTA CCTCACGGAC
    CACGTGGTGT TCCAGGATAA AGAATACAGC ATTGAAGAAA TAGAGGCTGG CCGAGTCCCG
    AACCCACACC TGGGCCCAGT GGAAGAGCGT CTGGCCTTGC ATGTCCTCCA GCAGCAGGGC
    CTGGTCCCTG AGCATGTGGA GTCCCGGCCT CTCTACAGCC CCCTGCAGCC AGACATTGAA
    CAGGGGAAGC TGCAGATGTG GATCGACCTA TTTCCCAAGG CCCTGGGGCG TCCCGGACCT
    CCCTTCAACA TCACCCCGCG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT CATCTGGAAC
    ACAAAGGACG TGATCCTGGA CGACCTCAGC ATCACAGGGG AGAAGATGAG TGATATTTAT
    GTGAAGGGTT GGATGGTTGG CTTTGAAGAG CACAAGCAAA AGACAGATGT GCATTACCGG
    TCCCTGGGAG GTGAAGGCAA CTTTAACTGG AGGTTCATTT TCCCCTTTGA CTACCTGCCA
    GCCGAGCAAG TCTGCACCGT CTCTAAGAAG GATGCTTTCT GGAGGCTGGA CAAGACTGAG
    AGCAAAATCC CAGCACGAGT GGTATTTCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAC
    GACTTTCTGG GCTCCTTGCA GTTGGATCTC AACCACATGC CCAAGCCTGC CAAGACAGCT
    GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACTTTCCACC CGGAGTGGTT TGTGTCCCTT
    TTTGAACAGA AAACAGTTAA GGGCTGGTGG CCCTGCGTAG CAGAAGAGGG CGAGAAGAAG
    ATCCTGGCAG GCAAGCTGGA AATGACCTTG GAGATCGTAG CAGAGAGTGA GCATGAGGAG
    CGGCCTGCTG GCCAGGGCCG GGATGAACCC AACATGAACC CCAAGCTCGA GGACCCAAGG
    CGCCCTGACA CCTCCTTCCT CTGGTTCACC TCTCCATACA AGACTATGAA GTTCATCCTG
    TGGCGGCGGT TCCGATGTGC CATTATCCTC TTCATCATTC TCTTCATCCT GCTACTATTC
    TTAGGCATCT TCATCTATGC TTTCCCGAAC TACGCTGCCA TGAAGCTGGT GAAGCCCTTC
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe19208
    Clone ID Related Accession (Same CDS sequence) XM_003432230.3 , XM_003432230.4
    Accession Version XM_003432230.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6348bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product dysferlin isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003432230.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG
    CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC
    GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG
    AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT
    CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG
    ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT
    GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT
    TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC
    GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT
    ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT
    GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC
    GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC
    AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC
    AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC
    ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG
    GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT
    GCCCCTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTGG TCAAGCCTTC TCCAGCCACG
    GACCTGGATG ACCATCTGGG CTTCCTTCCC ACCTTCGGGC CCTGCTTCGT CAATCTCTAT
    GGCAGCCCCA GGGAATTCAC TGGCTTCCCG GACCCCTACG CGGAACTCAA CATGGGCAAG
    GGGGAAGGCG TGGCCTACCG AGGTCGGCTT CTGCTCTCCC TGGAGACCAA GCTGGTGGAG
    CACAGCGGCG AGCAAAGGGT GGAGGACCTC CCTGCTGATG ACATCCTTCG GGTGGAGAAA
    TACCTCCGGA GGCGCAAGTA CTCTCTCTTT GCCGCCTTCT ACTCGGCCAC CATGCTCCAG
    GATGTCGACG ATGCCATCCA GTTTGAGGTC AGCATTGGGA ACTATGGCAA CAAGTTCGAC
    ACGACCTGTC TGCCACTGGC CTCCACCACC CAGTACAGCC GGGCAGTCTT TGATGGGTGC
    CACTACTACT ACTTGCCCTG GGGCAATGTG AAGCCTGTGG TGGTGCTGTC ATCCTACTGG
    GAAGACATCA GCCACAGAGT CGAGGCTCAG AACCAGCTGC TCAGGATCGC TGACCATCTG
    GAAGCTGGCC TGGAGCAAGT CCACTTGGCT TTAAAGGCGC AGTTCAACAC AGAGGATGTG
    GACTCCCTGT TGGCCCAGCT GATGGATGAG CTCATCTCAG ACTGCAGCCA GCCCCTGGGC
    AATGTCCAAG AGATACCCTC TGCCACCCAC CTGGACCGGC ACCTGTACAG GCTGCGCACC
    CGTCACCTAG GCCAGATTAC TGAGGCGGCC CTGGCTCTGA AACTCGGGCA TGGCAAGCTC
    CCTGCCGCCC TGGAGCAGGC TGAAGACTGG CTCCTGCGTC TCCGTGCCCT GGCTGATGAG
    CCCCAGAACA GCCTGCCCGA CATCATCATC TGGATGCTGC AGGGAAACAA GCGAGTGGCG
    TACCAGCGGG TGCCTGCGCA CGAGGTCCTC TTCTCCCGGC GGGGCGCCAG CTACTGTGGC
    AAGAACTGTG GGAAGCTGCA GACGATATTT CTGAAATATC CCATGGAGGG GGTGCCCGGA
    GCCCGGATGC CAGTACAAAT ACGGGTGAAA CTCTGGTTTG GGCTTTCTGT GGATGAGAAG
    GAGTTCAACC AGTTTGCTGA GGGAAAGCTG TCCGTCTTTG CTGAAACTTA TGAGAACCAG
    ACCAAACTGG CCCTGGTTGG GAACTGGGGC ACAACAGGCC TCACCTACCC CAAGTTTTCT
    GATGTCATGG GCAAGATCAA GCTACCCAAG GATAGCTTCC GCCCCTCAGC TGGCTGGACC
    TGGGCCGGAG ACTGGTTCGT GTGTCCAGAG AAGACCCTAC TCCATGACAT GGACGCGGGC
    CACCTGAGTT TTGTGGAGGA GGTGTTTGAG AACCAGACCC GGCTCCCTGG AGGCCAGTGG
    ATCTACATGA GTGACAACTA CACAGATGTG AATGGGGATA AGGTGCTTCC TAAGGATGAC
    ATTGAGTGCC CACTGGGCTG GAAGTGGGAA GATGAAGAGT GGTCCACGGA CCTCAATCGG
    GCCGTCGATG AGCAAGGCTG GGAGTACAGT ATCACCATCC CCCCGGATCG GAAGCCAAAG
    CACTGGGTTC CTGCTGAGAA GATGTATTAC ACGCACCGTC GGCGGCGCTG GGTCCGCCTG
    CGCAGAAGGG ACCTCAGCCA GATGGAGGCA TTGAAGAGGC ACAGACAGGC AGAGGCAGAG
    GGTGAGGGCT GGGAGTACGC CTCGCTCTTG GGCTGGAAGT TCCACCTGGA GTACCGCAAG
    ACTGATGCTT TCCGCCGCCG CCGCTGGCGC CGCCGCATGG AACCGCTGGA GAAGACGGGG
    CCTGCAGCCG TGTTTGCCCT TGAGGGGGCT CTGGGTGGTG TGGTAGATGA CAGGAGTGAA
    GATTCCGTGT CCGTGTCCAC CTTGAGCTTT GGAGTGAACA GACCCACGAT TTCCTGCATC
    TTTGATTATG GGAACCGCTA CCACCTACGC TGCTACATGT ACCAGGCTCG GGACCTGCCC
    TCGATGGACA AGGACTCTTT TTCTGATCCC TACGCCATCG TCTCCTTCCT GCACCAGAGC
    CAGAAGACAG TGGTGGCGAA GAACACTCTG AACCCCACCT GGGACCAGAC GCTTATCTTC
    TATGAGATTG AGATCTTTGG TGAGCCAGCC AGTATCGCTG AGCAGCCGCC CAGCATTGTG
    GTGGAGCTGT ATGACCACGA CACCTACGGT GCAGATGAGT TTATGGGACG CTGCATTTGT
    CAGCCCAGCC TGGAGCGGAC ACCCCGGCTG GCCTGGTTCC CACTGATTAG GGGCAGCCAG
    TCGGCGGGGG AACTGCTGGC CTCTTTTGAG CTCATCCAGA GAGAAAAGCC GGCCATTCAC
    CATATTCCTG GTTTTGAGGT ACAGGATATG TCAGGAATCC TGGAAGAGTC AGAGGACATA
    GACCTGCCCT ACCCGCCACC TCAGAGGGAG GCCAACATCT TCATGGTCCC CCAGAACATC
    AAGCCTGTTC TCCAGCGTAC TGCCATAGAG ATCCTGGCGT GGGGCCTGCG GAACATGAAG
    AGCTACCAGC TAGCCAGCAT CTCCTCCCCT AGCCTTGTGG TAGAGTGCGG GGGCCAGACA
    GTGCAGTCCT GTGTCATCAA GAACCTCCGG AAGAACCCCA ACTTCGACAT CTGTACCCTT
    TTCATGGAAG TGATGCTGCC CAAGGAGGAG GTCTACTGTC CCTCCATCGT GATCAAGGTC
    ATTGATAACC GCCAGTTTGG CCGCCGGCCT GTGGTGGGCC AGTGTACCAT CCGTTCCCTG
    GAGAGCTTCA TGTGTGACCT GTACTCCGAG GAGAATCCGT CCCCACAGGG CGGCCCAGAT
    GATGTGAGTT TACTCAGTCC TGGGGAAGAC GTGCTCATTG ACATCGATGA CAAGGAGCCC
    CTCATCCCCA TGCAGGAGGA AGAGTTCATC GACTGGTGGA GCAAATTCTT TGCCTCCACA
    GGGGAGAGGG AAAAGTGCGG CTCATACTTG GAGAAGGGCT TTGACACTCT AAAGGTCTAT
    GACACACAAC TGGAGAACGT GGAGGCCTTT GAGGGCCTGT CTGACTTTTG TAACACCTTC
    AAGCTCTACC GGGGCAAGAC TCAGGAGGAG ACAGAGGACC CATCTGTTAT TGGGGAGTTT
    AAGGGCCTCT TCAAAATCTA TCCCCTCCCA GAAGACCCGG CTGTTCCCAT GCCCCCGAGA
    CAATTCCACC AGCTGGCTGC CCAAGGGCCT CAGGAGTGCC TGGTCCGCAT CTATATCATC
    CGAGCATTTG GCCTTCAGCC CAAGGACCCC AATGGGAAGT GTGATCCTTA CATCAAGATC
    TCCATAGGGA AGAAATCAGT GAGTGACCAG GATAACTACA TTCCCTGTAC TCTGGAGCCT
    GTGTTTGGAA AGATGTTCGA GCTGACCTGC ACTCTGCCTC TGGAGAAGGA CCTGAAGATC
    ACACTCTATG ACTATGACCT CCTCTCCAAA GATGAAAAGA TTGGGGAGAC AGTCATTGAC
    CTGGAGGATA GGCTGCTGTC CAAGTTTGGG GCTCGCTGCG GACTCCCACA GACGTACTGT
    GTCTCTGGAC CCAACCAGTG GAGGGACCAG CTCCGCCCCT CCCAGCTTCT CCACCTCTTC
    TGCCAGCAGC GCAGACTCAA GGCTCCTGTG TACCTCACGG ACCACGTGGT GTTCCAGGAT
    AAAGAATACA GCATTGAAGA AATAGAGGCT GGCCGAGTCC CGAACCCACA CCTGGGCCCA
    GTGGAAGAGC GTCTGGCCTT GCATGTCCTC CAGCAGCAGG GCCTGGTCCC TGAGCATGTG
    GAGTCCCGGC CTCTCTACAG CCCCCTGCAG CCAGACATTG AACAGGGGAA GCTGCAGATG
    TGGATCGACC TATTTCCCAA GGCCCTGGGG CGTCCCGGAC CTCCCTTCAA CATCACCCCG
    CGGAGAGCCA GAAGGTTTTT CCTGCGTTGT ATCATCTGGA ACACAAAGGA CGTGATCCTG
    GACGACCTCA GCATCACAGG GGAGAAGATG AGTGATATTT ATGTGAAGGG TTGGATGGTT
    GGCTTTGAAG AGCACAAGCA AAAGACAGAT GTGCATTACC GGTCCCTGGG AGGTGAAGGC
    AACTTTAACT GGAGGTTCAT TTTCCCCTTT GACTACCTGC CAGCCGAGCA AGTCTGCACC
    GTCTCTAAGA AGGATGCTTT CTGGAGGCTG GACAAGACTG AGAGCAAAAT CCCAGCACGA
    GTGGTATTTC AGATCTGGGA CAATGACAAG TTCTCCTTTG ACGACTTTCT GGGCTCCTTG
    CAGTTGGATC TCAACCACAT GCCCAAGCCT GCCAAGACAG CTGAGAAGTG CTCCTTGGAC
    CAGCTGGATG ACACTTTCCA CCCGGAGTGG TTTGTGTCCC TTTTTGAACA GAAAACAGTT
    AAGGGCTGGT GGCCCTGCGT AGCAGAAGAG GGCGAGAAGA AGATCCTGGC AGGCAAGCTG
    GAAATGACCT TGGAGATCGT AGCAGAGAGT GAGCATGAGG AGCGGCCTGC TGGCCAGGGC
    CGGGATGAAC CCAACATGAA CCCCAAGCTC GAGGACCCAA GGCGCCCTGA CACCTCCTTC
    CTCTGGTTCA CCTCTCCATA CAAGACTATG AAGTTCATCC TGTGGCGGCG GTTCCGATGT
    GCCATTATCC TCTTCATCAT TCTCTTCATC CTGCTACTAT TCTTAGGCAT CTTCATCTAT
    GCTTTCCCGA ACTACGCTGC CATGAAGCTG GTGAAGCCCT TCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003432278.1
    CDS51..6398
    Translation

    Target ORF information:

    RefSeq Version XM_003432230.3
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris dysferlin (DYSF), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003432230.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG
    CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC
    GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG
    AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT
    CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG
    ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT
    GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT
    TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC
    GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT
    ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT
    GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC
    GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC
    AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC
    AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC
    ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG
    GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT
    GCCCCTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTGG TCAAGCCTTC TCCAGCCACG
    GACCTGGATG ACCATCTGGG CTTCCTTCCC ACCTTCGGGC CCTGCTTCGT CAATCTCTAT
    GGCAGCCCCA GGGAATTCAC TGGCTTCCCG GACCCCTACG CGGAACTCAA CATGGGCAAG
    GGGGAAGGCG TGGCCTACCG AGGTCGGCTT CTGCTCTCCC TGGAGACCAA GCTGGTGGAG
    CACAGCGGCG AGCAAAGGGT GGAGGACCTC CCTGCTGATG ACATCCTTCG GGTGGAGAAA
    TACCTCCGGA GGCGCAAGTA CTCTCTCTTT GCCGCCTTCT ACTCGGCCAC CATGCTCCAG
    GATGTCGACG ATGCCATCCA GTTTGAGGTC AGCATTGGGA ACTATGGCAA CAAGTTCGAC
    ACGACCTGTC TGCCACTGGC CTCCACCACC CAGTACAGCC GGGCAGTCTT TGATGGGTGC
    CACTACTACT ACTTGCCCTG GGGCAATGTG AAGCCTGTGG TGGTGCTGTC ATCCTACTGG
    GAAGACATCA GCCACAGAGT CGAGGCTCAG AACCAGCTGC TCAGGATCGC TGACCATCTG
    GAAGCTGGCC TGGAGCAAGT CCACTTGGCT TTAAAGGCGC AGTTCAACAC AGAGGATGTG
    GACTCCCTGT TGGCCCAGCT GATGGATGAG CTCATCTCAG ACTGCAGCCA GCCCCTGGGC
    AATGTCCAAG AGATACCCTC TGCCACCCAC CTGGACCGGC ACCTGTACAG GCTGCGCACC
    CGTCACCTAG GCCAGATTAC TGAGGCGGCC CTGGCTCTGA AACTCGGGCA TGGCAAGCTC
    CCTGCCGCCC TGGAGCAGGC TGAAGACTGG CTCCTGCGTC TCCGTGCCCT GGCTGATGAG
    CCCCAGAACA GCCTGCCCGA CATCATCATC TGGATGCTGC AGGGAAACAA GCGAGTGGCG
    TACCAGCGGG TGCCTGCGCA CGAGGTCCTC TTCTCCCGGC GGGGCGCCAG CTACTGTGGC
    AAGAACTGTG GGAAGCTGCA GACGATATTT CTGAAATATC CCATGGAGGG GGTGCCCGGA
    GCCCGGATGC CAGTACAAAT ACGGGTGAAA CTCTGGTTTG GGCTTTCTGT GGATGAGAAG
    GAGTTCAACC AGTTTGCTGA GGGAAAGCTG TCCGTCTTTG CTGAAACTTA TGAGAACCAG
    ACCAAACTGG CCCTGGTTGG GAACTGGGGC ACAACAGGCC TCACCTACCC CAAGTTTTCT
    GATGTCATGG GCAAGATCAA GCTACCCAAG GATAGCTTCC GCCCCTCAGC TGGCTGGACC
    TGGGCCGGAG ACTGGTTCGT GTGTCCAGAG AAGACCCTAC TCCATGACAT GGACGCGGGC
    CACCTGAGTT TTGTGGAGGA GGTGTTTGAG AACCAGACCC GGCTCCCTGG AGGCCAGTGG
    ATCTACATGA GTGACAACTA CACAGATGTG AATGGGGATA AGGTGCTTCC TAAGGATGAC
    ATTGAGTGCC CACTGGGCTG GAAGTGGGAA GATGAAGAGT GGTCCACGGA CCTCAATCGG
    GCCGTCGATG AGCAAGGCTG GGAGTACAGT ATCACCATCC CCCCGGATCG GAAGCCAAAG
    CACTGGGTTC CTGCTGAGAA GATGTATTAC ACGCACCGTC GGCGGCGCTG GGTCCGCCTG
    CGCAGAAGGG ACCTCAGCCA GATGGAGGCA TTGAAGAGGC ACAGACAGGC AGAGGCAGAG
    GGTGAGGGCT GGGAGTACGC CTCGCTCTTG GGCTGGAAGT TCCACCTGGA GTACCGCAAG
    ACTGATGCTT TCCGCCGCCG CCGCTGGCGC CGCCGCATGG AACCGCTGGA GAAGACGGGG
    CCTGCAGCCG TGTTTGCCCT TGAGGGGGCT CTGGGTGGTG TGGTAGATGA CAGGAGTGAA
    GATTCCGTGT CCGTGTCCAC CTTGAGCTTT GGAGTGAACA GACCCACGAT TTCCTGCATC
    TTTGATTATG GGAACCGCTA CCACCTACGC TGCTACATGT ACCAGGCTCG GGACCTGCCC
    TCGATGGACA AGGACTCTTT TTCTGATCCC TACGCCATCG TCTCCTTCCT GCACCAGAGC
    CAGAAGACAG TGGTGGCGAA GAACACTCTG AACCCCACCT GGGACCAGAC GCTTATCTTC
    TATGAGATTG AGATCTTTGG TGAGCCAGCC AGTATCGCTG AGCAGCCGCC CAGCATTGTG
    GTGGAGCTGT ATGACCACGA CACCTACGGT GCAGATGAGT TTATGGGACG CTGCATTTGT
    CAGCCCAGCC TGGAGCGGAC ACCCCGGCTG GCCTGGTTCC CACTGATTAG GGGCAGCCAG
    TCGGCGGGGG AACTGCTGGC CTCTTTTGAG CTCATCCAGA GAGAAAAGCC GGCCATTCAC
    CATATTCCTG GTTTTGAGGT ACAGGATATG TCAGGAATCC TGGAAGAGTC AGAGGACATA
    GACCTGCCCT ACCCGCCACC TCAGAGGGAG GCCAACATCT TCATGGTCCC CCAGAACATC
    AAGCCTGTTC TCCAGCGTAC TGCCATAGAG ATCCTGGCGT GGGGCCTGCG GAACATGAAG
    AGCTACCAGC TAGCCAGCAT CTCCTCCCCT AGCCTTGTGG TAGAGTGCGG GGGCCAGACA
    GTGCAGTCCT GTGTCATCAA GAACCTCCGG AAGAACCCCA ACTTCGACAT CTGTACCCTT
    TTCATGGAAG TGATGCTGCC CAAGGAGGAG GTCTACTGTC CCTCCATCGT GATCAAGGTC
    ATTGATAACC GCCAGTTTGG CCGCCGGCCT GTGGTGGGCC AGTGTACCAT CCGTTCCCTG
    GAGAGCTTCA TGTGTGACCT GTACTCCGAG GAGAATCCGT CCCCACAGGG CGGCCCAGAT
    GATGTGAGTT TACTCAGTCC TGGGGAAGAC GTGCTCATTG ACATCGATGA CAAGGAGCCC
    CTCATCCCCA TGCAGGAGGA AGAGTTCATC GACTGGTGGA GCAAATTCTT TGCCTCCACA
    GGGGAGAGGG AAAAGTGCGG CTCATACTTG GAGAAGGGCT TTGACACTCT AAAGGTCTAT
    GACACACAAC TGGAGAACGT GGAGGCCTTT GAGGGCCTGT CTGACTTTTG TAACACCTTC
    AAGCTCTACC GGGGCAAGAC TCAGGAGGAG ACAGAGGACC CATCTGTTAT TGGGGAGTTT
    AAGGGCCTCT TCAAAATCTA TCCCCTCCCA GAAGACCCGG CTGTTCCCAT GCCCCCGAGA
    CAATTCCACC AGCTGGCTGC CCAAGGGCCT CAGGAGTGCC TGGTCCGCAT CTATATCATC
    CGAGCATTTG GCCTTCAGCC CAAGGACCCC AATGGGAAGT GTGATCCTTA CATCAAGATC
    TCCATAGGGA AGAAATCAGT GAGTGACCAG GATAACTACA TTCCCTGTAC TCTGGAGCCT
    GTGTTTGGAA AGATGTTCGA GCTGACCTGC ACTCTGCCTC TGGAGAAGGA CCTGAAGATC
    ACACTCTATG ACTATGACCT CCTCTCCAAA GATGAAAAGA TTGGGGAGAC AGTCATTGAC
    CTGGAGGATA GGCTGCTGTC CAAGTTTGGG GCTCGCTGCG GACTCCCACA GACGTACTGT
    GTCTCTGGAC CCAACCAGTG GAGGGACCAG CTCCGCCCCT CCCAGCTTCT CCACCTCTTC
    TGCCAGCAGC GCAGACTCAA GGCTCCTGTG TACCTCACGG ACCACGTGGT GTTCCAGGAT
    AAAGAATACA GCATTGAAGA AATAGAGGCT GGCCGAGTCC CGAACCCACA CCTGGGCCCA
    GTGGAAGAGC GTCTGGCCTT GCATGTCCTC CAGCAGCAGG GCCTGGTCCC TGAGCATGTG
    GAGTCCCGGC CTCTCTACAG CCCCCTGCAG CCAGACATTG AACAGGGGAA GCTGCAGATG
    TGGATCGACC TATTTCCCAA GGCCCTGGGG CGTCCCGGAC CTCCCTTCAA CATCACCCCG
    CGGAGAGCCA GAAGGTTTTT CCTGCGTTGT ATCATCTGGA ACACAAAGGA CGTGATCCTG
    GACGACCTCA GCATCACAGG GGAGAAGATG AGTGATATTT ATGTGAAGGG TTGGATGGTT
    GGCTTTGAAG AGCACAAGCA AAAGACAGAT GTGCATTACC GGTCCCTGGG AGGTGAAGGC
    AACTTTAACT GGAGGTTCAT TTTCCCCTTT GACTACCTGC CAGCCGAGCA AGTCTGCACC
    GTCTCTAAGA AGGATGCTTT CTGGAGGCTG GACAAGACTG AGAGCAAAAT CCCAGCACGA
    GTGGTATTTC AGATCTGGGA CAATGACAAG TTCTCCTTTG ACGACTTTCT GGGCTCCTTG
    CAGTTGGATC TCAACCACAT GCCCAAGCCT GCCAAGACAG CTGAGAAGTG CTCCTTGGAC
    CAGCTGGATG ACACTTTCCA CCCGGAGTGG TTTGTGTCCC TTTTTGAACA GAAAACAGTT
    AAGGGCTGGT GGCCCTGCGT AGCAGAAGAG GGCGAGAAGA AGATCCTGGC AGGCAAGCTG
    GAAATGACCT TGGAGATCGT AGCAGAGAGT GAGCATGAGG AGCGGCCTGC TGGCCAGGGC
    CGGGATGAAC CCAACATGAA CCCCAAGCTC GAGGACCCAA GGCGCCCTGA CACCTCCTTC
    CTCTGGTTCA CCTCTCCATA CAAGACTATG AAGTTCATCC TGTGGCGGCG GTTCCGATGT
    GCCATTATCC TCTTCATCAT TCTCTTCATC CTGCTACTAT TCTTAGGCAT CTTCATCTAT
    GCTTTCCCGA ACTACGCTGC CATGAAGCTG GTGAAGCCCT TCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe19208
    Clone ID Related Accession (Same CDS sequence) XM_003432230.3 , XM_003432230.4
    Accession Version XM_003432230.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6348bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product dysferlin isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003432230.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG
    CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC
    GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG
    AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT
    CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG
    ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT
    GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT
    TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC
    GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT
    ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT
    GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC
    GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC
    AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC
    AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC
    ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG
    GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT
    GCCCCTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTGG TCAAGCCTTC TCCAGCCACG
    GACCTGGATG ACCATCTGGG CTTCCTTCCC ACCTTCGGGC CCTGCTTCGT CAATCTCTAT
    GGCAGCCCCA GGGAATTCAC TGGCTTCCCG GACCCCTACG CGGAACTCAA CATGGGCAAG
    GGGGAAGGCG TGGCCTACCG AGGTCGGCTT CTGCTCTCCC TGGAGACCAA GCTGGTGGAG
    CACAGCGGCG AGCAAAGGGT GGAGGACCTC CCTGCTGATG ACATCCTTCG GGTGGAGAAA
    TACCTCCGGA GGCGCAAGTA CTCTCTCTTT GCCGCCTTCT ACTCGGCCAC CATGCTCCAG
    GATGTCGACG ATGCCATCCA GTTTGAGGTC AGCATTGGGA ACTATGGCAA CAAGTTCGAC
    ACGACCTGTC TGCCACTGGC CTCCACCACC CAGTACAGCC GGGCAGTCTT TGATGGGTGC
    CACTACTACT ACTTGCCCTG GGGCAATGTG AAGCCTGTGG TGGTGCTGTC ATCCTACTGG
    GAAGACATCA GCCACAGAGT CGAGGCTCAG AACCAGCTGC TCAGGATCGC TGACCATCTG
    GAAGCTGGCC TGGAGCAAGT CCACTTGGCT TTAAAGGCGC AGTTCAACAC AGAGGATGTG
    GACTCCCTGT TGGCCCAGCT GATGGATGAG CTCATCTCAG ACTGCAGCCA GCCCCTGGGC
    AATGTCCAAG AGATACCCTC TGCCACCCAC CTGGACCGGC ACCTGTACAG GCTGCGCACC
    CGTCACCTAG GCCAGATTAC TGAGGCGGCC CTGGCTCTGA AACTCGGGCA TGGCAAGCTC
    CCTGCCGCCC TGGAGCAGGC TGAAGACTGG CTCCTGCGTC TCCGTGCCCT GGCTGATGAG
    CCCCAGAACA GCCTGCCCGA CATCATCATC TGGATGCTGC AGGGAAACAA GCGAGTGGCG
    TACCAGCGGG TGCCTGCGCA CGAGGTCCTC TTCTCCCGGC GGGGCGCCAG CTACTGTGGC
    AAGAACTGTG GGAAGCTGCA GACGATATTT CTGAAATATC CCATGGAGGG GGTGCCCGGA
    GCCCGGATGC CAGTACAAAT ACGGGTGAAA CTCTGGTTTG GGCTTTCTGT GGATGAGAAG
    GAGTTCAACC AGTTTGCTGA GGGAAAGCTG TCCGTCTTTG CTGAAACTTA TGAGAACCAG
    ACCAAACTGG CCCTGGTTGG GAACTGGGGC ACAACAGGCC TCACCTACCC CAAGTTTTCT
    GATGTCATGG GCAAGATCAA GCTACCCAAG GATAGCTTCC GCCCCTCAGC TGGCTGGACC
    TGGGCCGGAG ACTGGTTCGT GTGTCCAGAG AAGACCCTAC TCCATGACAT GGACGCGGGC
    CACCTGAGTT TTGTGGAGGA GGTGTTTGAG AACCAGACCC GGCTCCCTGG AGGCCAGTGG
    ATCTACATGA GTGACAACTA CACAGATGTG AATGGGGATA AGGTGCTTCC TAAGGATGAC
    ATTGAGTGCC CACTGGGCTG GAAGTGGGAA GATGAAGAGT GGTCCACGGA CCTCAATCGG
    GCCGTCGATG AGCAAGGCTG GGAGTACAGT ATCACCATCC CCCCGGATCG GAAGCCAAAG
    CACTGGGTTC CTGCTGAGAA GATGTATTAC ACGCACCGTC GGCGGCGCTG GGTCCGCCTG
    CGCAGAAGGG ACCTCAGCCA GATGGAGGCA TTGAAGAGGC ACAGACAGGC AGAGGCAGAG
    GGTGAGGGCT GGGAGTACGC CTCGCTCTTG GGCTGGAAGT TCCACCTGGA GTACCGCAAG
    ACTGATGCTT TCCGCCGCCG CCGCTGGCGC CGCCGCATGG AACCGCTGGA GAAGACGGGG
    CCTGCAGCCG TGTTTGCCCT TGAGGGGGCT CTGGGTGGTG TGGTAGATGA CAGGAGTGAA
    GATTCCGTGT CCGTGTCCAC CTTGAGCTTT GGAGTGAACA GACCCACGAT TTCCTGCATC
    TTTGATTATG GGAACCGCTA CCACCTACGC TGCTACATGT ACCAGGCTCG GGACCTGCCC
    TCGATGGACA AGGACTCTTT TTCTGATCCC TACGCCATCG TCTCCTTCCT GCACCAGAGC
    CAGAAGACAG TGGTGGCGAA GAACACTCTG AACCCCACCT GGGACCAGAC GCTTATCTTC
    TATGAGATTG AGATCTTTGG TGAGCCAGCC AGTATCGCTG AGCAGCCGCC CAGCATTGTG
    GTGGAGCTGT ATGACCACGA CACCTACGGT GCAGATGAGT TTATGGGACG CTGCATTTGT
    CAGCCCAGCC TGGAGCGGAC ACCCCGGCTG GCCTGGTTCC CACTGATTAG GGGCAGCCAG
    TCGGCGGGGG AACTGCTGGC CTCTTTTGAG CTCATCCAGA GAGAAAAGCC GGCCATTCAC
    CATATTCCTG GTTTTGAGGT ACAGGATATG TCAGGAATCC TGGAAGAGTC AGAGGACATA
    GACCTGCCCT ACCCGCCACC TCAGAGGGAG GCCAACATCT TCATGGTCCC CCAGAACATC
    AAGCCTGTTC TCCAGCGTAC TGCCATAGAG ATCCTGGCGT GGGGCCTGCG GAACATGAAG
    AGCTACCAGC TAGCCAGCAT CTCCTCCCCT AGCCTTGTGG TAGAGTGCGG GGGCCAGACA
    GTGCAGTCCT GTGTCATCAA GAACCTCCGG AAGAACCCCA ACTTCGACAT CTGTACCCTT
    TTCATGGAAG TGATGCTGCC CAAGGAGGAG GTCTACTGTC CCTCCATCGT GATCAAGGTC
    ATTGATAACC GCCAGTTTGG CCGCCGGCCT GTGGTGGGCC AGTGTACCAT CCGTTCCCTG
    GAGAGCTTCA TGTGTGACCT GTACTCCGAG GAGAATCCGT CCCCACAGGG CGGCCCAGAT
    GATGTGAGTT TACTCAGTCC TGGGGAAGAC GTGCTCATTG ACATCGATGA CAAGGAGCCC
    CTCATCCCCA TGCAGGAGGA AGAGTTCATC GACTGGTGGA GCAAATTCTT TGCCTCCACA
    GGGGAGAGGG AAAAGTGCGG CTCATACTTG GAGAAGGGCT TTGACACTCT AAAGGTCTAT
    GACACACAAC TGGAGAACGT GGAGGCCTTT GAGGGCCTGT CTGACTTTTG TAACACCTTC
    AAGCTCTACC GGGGCAAGAC TCAGGAGGAG ACAGAGGACC CATCTGTTAT TGGGGAGTTT
    AAGGGCCTCT TCAAAATCTA TCCCCTCCCA GAAGACCCGG CTGTTCCCAT GCCCCCGAGA
    CAATTCCACC AGCTGGCTGC CCAAGGGCCT CAGGAGTGCC TGGTCCGCAT CTATATCATC
    CGAGCATTTG GCCTTCAGCC CAAGGACCCC AATGGGAAGT GTGATCCTTA CATCAAGATC
    TCCATAGGGA AGAAATCAGT GAGTGACCAG GATAACTACA TTCCCTGTAC TCTGGAGCCT
    GTGTTTGGAA AGATGTTCGA GCTGACCTGC ACTCTGCCTC TGGAGAAGGA CCTGAAGATC
    ACACTCTATG ACTATGACCT CCTCTCCAAA GATGAAAAGA TTGGGGAGAC AGTCATTGAC
    CTGGAGGATA GGCTGCTGTC CAAGTTTGGG GCTCGCTGCG GACTCCCACA GACGTACTGT
    GTCTCTGGAC CCAACCAGTG GAGGGACCAG CTCCGCCCCT CCCAGCTTCT CCACCTCTTC
    TGCCAGCAGC GCAGACTCAA GGCTCCTGTG TACCTCACGG ACCACGTGGT GTTCCAGGAT
    AAAGAATACA GCATTGAAGA AATAGAGGCT GGCCGAGTCC CGAACCCACA CCTGGGCCCA
    GTGGAAGAGC GTCTGGCCTT GCATGTCCTC CAGCAGCAGG GCCTGGTCCC TGAGCATGTG
    GAGTCCCGGC CTCTCTACAG CCCCCTGCAG CCAGACATTG AACAGGGGAA GCTGCAGATG
    TGGATCGACC TATTTCCCAA GGCCCTGGGG CGTCCCGGAC CTCCCTTCAA CATCACCCCG
    CGGAGAGCCA GAAGGTTTTT CCTGCGTTGT ATCATCTGGA ACACAAAGGA CGTGATCCTG
    GACGACCTCA GCATCACAGG GGAGAAGATG AGTGATATTT ATGTGAAGGG TTGGATGGTT
    GGCTTTGAAG AGCACAAGCA AAAGACAGAT GTGCATTACC GGTCCCTGGG AGGTGAAGGC
    AACTTTAACT GGAGGTTCAT TTTCCCCTTT GACTACCTGC CAGCCGAGCA AGTCTGCACC
    GTCTCTAAGA AGGATGCTTT CTGGAGGCTG GACAAGACTG AGAGCAAAAT CCCAGCACGA
    GTGGTATTTC AGATCTGGGA CAATGACAAG TTCTCCTTTG ACGACTTTCT GGGCTCCTTG
    CAGTTGGATC TCAACCACAT GCCCAAGCCT GCCAAGACAG CTGAGAAGTG CTCCTTGGAC
    CAGCTGGATG ACACTTTCCA CCCGGAGTGG TTTGTGTCCC TTTTTGAACA GAAAACAGTT
    AAGGGCTGGT GGCCCTGCGT AGCAGAAGAG GGCGAGAAGA AGATCCTGGC AGGCAAGCTG
    GAAATGACCT TGGAGATCGT AGCAGAGAGT GAGCATGAGG AGCGGCCTGC TGGCCAGGGC
    CGGGATGAAC CCAACATGAA CCCCAAGCTC GAGGACCCAA GGCGCCCTGA CACCTCCTTC
    CTCTGGTTCA CCTCTCCATA CAAGACTATG AAGTTCATCC TGTGGCGGCG GTTCCGATGT
    GCCATTATCC TCTTCATCAT TCTCTTCATC CTGCTACTAT TCTTAGGCAT CTTCATCTAT
    GCTTTCCCGA ACTACGCTGC CATGAAGCTG GTGAAGCCCT TCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003432278.1
    CDS23..6370
    Translation

    Target ORF information:

    RefSeq Version XM_003432230.4
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris dysferlin (DYSF), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003432230.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG
    CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC
    GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG
    AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT
    CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG
    ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT
    GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT
    TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC
    GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT
    ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT
    GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC
    GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC
    AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC
    AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC
    ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG
    GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT
    GCCCCTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTGG TCAAGCCTTC TCCAGCCACG
    GACCTGGATG ACCATCTGGG CTTCCTTCCC ACCTTCGGGC CCTGCTTCGT CAATCTCTAT
    GGCAGCCCCA GGGAATTCAC TGGCTTCCCG GACCCCTACG CGGAACTCAA CATGGGCAAG
    GGGGAAGGCG TGGCCTACCG AGGTCGGCTT CTGCTCTCCC TGGAGACCAA GCTGGTGGAG
    CACAGCGGCG AGCAAAGGGT GGAGGACCTC CCTGCTGATG ACATCCTTCG GGTGGAGAAA
    TACCTCCGGA GGCGCAAGTA CTCTCTCTTT GCCGCCTTCT ACTCGGCCAC CATGCTCCAG
    GATGTCGACG ATGCCATCCA GTTTGAGGTC AGCATTGGGA ACTATGGCAA CAAGTTCGAC
    ACGACCTGTC TGCCACTGGC CTCCACCACC CAGTACAGCC GGGCAGTCTT TGATGGGTGC
    CACTACTACT ACTTGCCCTG GGGCAATGTG AAGCCTGTGG TGGTGCTGTC ATCCTACTGG
    GAAGACATCA GCCACAGAGT CGAGGCTCAG AACCAGCTGC TCAGGATCGC TGACCATCTG
    GAAGCTGGCC TGGAGCAAGT CCACTTGGCT TTAAAGGCGC AGTTCAACAC AGAGGATGTG
    GACTCCCTGT TGGCCCAGCT GATGGATGAG CTCATCTCAG ACTGCAGCCA GCCCCTGGGC
    AATGTCCAAG AGATACCCTC TGCCACCCAC CTGGACCGGC ACCTGTACAG GCTGCGCACC
    CGTCACCTAG GCCAGATTAC TGAGGCGGCC CTGGCTCTGA AACTCGGGCA TGGCAAGCTC
    CCTGCCGCCC TGGAGCAGGC TGAAGACTGG CTCCTGCGTC TCCGTGCCCT GGCTGATGAG
    CCCCAGAACA GCCTGCCCGA CATCATCATC TGGATGCTGC AGGGAAACAA GCGAGTGGCG
    TACCAGCGGG TGCCTGCGCA CGAGGTCCTC TTCTCCCGGC GGGGCGCCAG CTACTGTGGC
    AAGAACTGTG GGAAGCTGCA GACGATATTT CTGAAATATC CCATGGAGGG GGTGCCCGGA
    GCCCGGATGC CAGTACAAAT ACGGGTGAAA CTCTGGTTTG GGCTTTCTGT GGATGAGAAG
    GAGTTCAACC AGTTTGCTGA GGGAAAGCTG TCCGTCTTTG CTGAAACTTA TGAGAACCAG
    ACCAAACTGG CCCTGGTTGG GAACTGGGGC ACAACAGGCC TCACCTACCC CAAGTTTTCT
    GATGTCATGG GCAAGATCAA GCTACCCAAG GATAGCTTCC GCCCCTCAGC TGGCTGGACC
    TGGGCCGGAG ACTGGTTCGT GTGTCCAGAG AAGACCCTAC TCCATGACAT GGACGCGGGC
    CACCTGAGTT TTGTGGAGGA GGTGTTTGAG AACCAGACCC GGCTCCCTGG AGGCCAGTGG
    ATCTACATGA GTGACAACTA CACAGATGTG AATGGGGATA AGGTGCTTCC TAAGGATGAC
    ATTGAGTGCC CACTGGGCTG GAAGTGGGAA GATGAAGAGT GGTCCACGGA CCTCAATCGG
    GCCGTCGATG AGCAAGGCTG GGAGTACAGT ATCACCATCC CCCCGGATCG GAAGCCAAAG
    CACTGGGTTC CTGCTGAGAA GATGTATTAC ACGCACCGTC GGCGGCGCTG GGTCCGCCTG
    CGCAGAAGGG ACCTCAGCCA GATGGAGGCA TTGAAGAGGC ACAGACAGGC AGAGGCAGAG
    GGTGAGGGCT GGGAGTACGC CTCGCTCTTG GGCTGGAAGT TCCACCTGGA GTACCGCAAG
    ACTGATGCTT TCCGCCGCCG CCGCTGGCGC CGCCGCATGG AACCGCTGGA GAAGACGGGG
    CCTGCAGCCG TGTTTGCCCT TGAGGGGGCT CTGGGTGGTG TGGTAGATGA CAGGAGTGAA
    GATTCCGTGT CCGTGTCCAC CTTGAGCTTT GGAGTGAACA GACCCACGAT TTCCTGCATC
    TTTGATTATG GGAACCGCTA CCACCTACGC TGCTACATGT ACCAGGCTCG GGACCTGCCC
    TCGATGGACA AGGACTCTTT TTCTGATCCC TACGCCATCG TCTCCTTCCT GCACCAGAGC
    CAGAAGACAG TGGTGGCGAA GAACACTCTG AACCCCACCT GGGACCAGAC GCTTATCTTC
    TATGAGATTG AGATCTTTGG TGAGCCAGCC AGTATCGCTG AGCAGCCGCC CAGCATTGTG
    GTGGAGCTGT ATGACCACGA CACCTACGGT GCAGATGAGT TTATGGGACG CTGCATTTGT
    CAGCCCAGCC TGGAGCGGAC ACCCCGGCTG GCCTGGTTCC CACTGATTAG GGGCAGCCAG
    TCGGCGGGGG AACTGCTGGC CTCTTTTGAG CTCATCCAGA GAGAAAAGCC GGCCATTCAC
    CATATTCCTG GTTTTGAGGT ACAGGATATG TCAGGAATCC TGGAAGAGTC AGAGGACATA
    GACCTGCCCT ACCCGCCACC TCAGAGGGAG GCCAACATCT TCATGGTCCC CCAGAACATC
    AAGCCTGTTC TCCAGCGTAC TGCCATAGAG ATCCTGGCGT GGGGCCTGCG GAACATGAAG
    AGCTACCAGC TAGCCAGCAT CTCCTCCCCT AGCCTTGTGG TAGAGTGCGG GGGCCAGACA
    GTGCAGTCCT GTGTCATCAA GAACCTCCGG AAGAACCCCA ACTTCGACAT CTGTACCCTT
    TTCATGGAAG TGATGCTGCC CAAGGAGGAG GTCTACTGTC CCTCCATCGT GATCAAGGTC
    ATTGATAACC GCCAGTTTGG CCGCCGGCCT GTGGTGGGCC AGTGTACCAT CCGTTCCCTG
    GAGAGCTTCA TGTGTGACCT GTACTCCGAG GAGAATCCGT CCCCACAGGG CGGCCCAGAT
    GATGTGAGTT TACTCAGTCC TGGGGAAGAC GTGCTCATTG ACATCGATGA CAAGGAGCCC
    CTCATCCCCA TGCAGGAGGA AGAGTTCATC GACTGGTGGA GCAAATTCTT TGCCTCCACA
    GGGGAGAGGG AAAAGTGCGG CTCATACTTG GAGAAGGGCT TTGACACTCT AAAGGTCTAT
    GACACACAAC TGGAGAACGT GGAGGCCTTT GAGGGCCTGT CTGACTTTTG TAACACCTTC
    AAGCTCTACC GGGGCAAGAC TCAGGAGGAG ACAGAGGACC CATCTGTTAT TGGGGAGTTT
    AAGGGCCTCT TCAAAATCTA TCCCCTCCCA GAAGACCCGG CTGTTCCCAT GCCCCCGAGA
    CAATTCCACC AGCTGGCTGC CCAAGGGCCT CAGGAGTGCC TGGTCCGCAT CTATATCATC
    CGAGCATTTG GCCTTCAGCC CAAGGACCCC AATGGGAAGT GTGATCCTTA CATCAAGATC
    TCCATAGGGA AGAAATCAGT GAGTGACCAG GATAACTACA TTCCCTGTAC TCTGGAGCCT
    GTGTTTGGAA AGATGTTCGA GCTGACCTGC ACTCTGCCTC TGGAGAAGGA CCTGAAGATC
    ACACTCTATG ACTATGACCT CCTCTCCAAA GATGAAAAGA TTGGGGAGAC AGTCATTGAC
    CTGGAGGATA GGCTGCTGTC CAAGTTTGGG GCTCGCTGCG GACTCCCACA GACGTACTGT
    GTCTCTGGAC CCAACCAGTG GAGGGACCAG CTCCGCCCCT CCCAGCTTCT CCACCTCTTC
    TGCCAGCAGC GCAGACTCAA GGCTCCTGTG TACCTCACGG ACCACGTGGT GTTCCAGGAT
    AAAGAATACA GCATTGAAGA AATAGAGGCT GGCCGAGTCC CGAACCCACA CCTGGGCCCA
    GTGGAAGAGC GTCTGGCCTT GCATGTCCTC CAGCAGCAGG GCCTGGTCCC TGAGCATGTG
    GAGTCCCGGC CTCTCTACAG CCCCCTGCAG CCAGACATTG AACAGGGGAA GCTGCAGATG
    TGGATCGACC TATTTCCCAA GGCCCTGGGG CGTCCCGGAC CTCCCTTCAA CATCACCCCG
    CGGAGAGCCA GAAGGTTTTT CCTGCGTTGT ATCATCTGGA ACACAAAGGA CGTGATCCTG
    GACGACCTCA GCATCACAGG GGAGAAGATG AGTGATATTT ATGTGAAGGG TTGGATGGTT
    GGCTTTGAAG AGCACAAGCA AAAGACAGAT GTGCATTACC GGTCCCTGGG AGGTGAAGGC
    AACTTTAACT GGAGGTTCAT TTTCCCCTTT GACTACCTGC CAGCCGAGCA AGTCTGCACC
    GTCTCTAAGA AGGATGCTTT CTGGAGGCTG GACAAGACTG AGAGCAAAAT CCCAGCACGA
    GTGGTATTTC AGATCTGGGA CAATGACAAG TTCTCCTTTG ACGACTTTCT GGGCTCCTTG
    CAGTTGGATC TCAACCACAT GCCCAAGCCT GCCAAGACAG CTGAGAAGTG CTCCTTGGAC
    CAGCTGGATG ACACTTTCCA CCCGGAGTGG TTTGTGTCCC TTTTTGAACA GAAAACAGTT
    AAGGGCTGGT GGCCCTGCGT AGCAGAAGAG GGCGAGAAGA AGATCCTGGC AGGCAAGCTG
    GAAATGACCT TGGAGATCGT AGCAGAGAGT GAGCATGAGG AGCGGCCTGC TGGCCAGGGC
    CGGGATGAAC CCAACATGAA CCCCAAGCTC GAGGACCCAA GGCGCCCTGA CACCTCCTTC
    CTCTGGTTCA CCTCTCCATA CAAGACTATG AAGTTCATCC TGTGGCGGCG GTTCCGATGT
    GCCATTATCC TCTTCATCAT TCTCTTCATC CTGCTACTAT TCTTAGGCAT CTTCATCTAT
    GCTTTCCCGA ACTACGCTGC CATGAAGCTG GTGAAGCCCT TCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe19209
    Clone ID Related Accession (Same CDS sequence) XM_003432225.4 , XM_003432225.3
    Accession Version XM_003432225.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6345bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product dysferlin isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003432225.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC
    CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC
    TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA
    CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGGTG GGGGACAGAG CCGGGCTGAG
    ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GATACAAGAT ACTCTGGAAA GAAGTGGCTG
    GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC TGGAGACGAG
    GGAGAGCCAT TACTGGATCA GAGTGGCGTC CCAGGCCCTG GGGCTCCCAC CACCCCGAGG
    AAGCCACCTT CCCATCTGCC CCCCTACCAC CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC
    GCAGCCAGAA AGCTGCTGTC CGACAAACCG CAGGACTTCC AGATCAGAGT GCAAGTGATC
    GAGGGCCGCC AGCTGCCAGG GGTGAATATC AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG
    CAGACCAAGA GGACACGGAT CCACAAGGGA AACAGCCCAC TATTTAACGA GACTCTTTTC
    TTCAATGTGT TTGACTCTCC TTCGGAGCTG TTTGATGAGC CCATCTTTAT CACGGTCGTA
    GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC
    ATCTACAGAG AGCCCCGACA TGCTTATCTG AGGAAGTGGC TGCTACTCTC CAACCCTGAT
    GACTTCTCTG TTGGGGCCAG AGGTTACCTG AAAGCAAGCC TTTATGTGCT GGGGCCCGGA
    GATGAAGCGC CTCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT TGAAAACAAT
    CTGCTCAGGC CAACTGGCGT GGCCCTGCGA GGAGCCCACT TCTGCCTGAA GATCTTCAGA
    GCGGAGGACT TACCACAAAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC
    TTTGACAGCA ACAAGAAGAA TTTGGTAGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA
    ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACT
    CTGCCTGCCA TGTTTCCCTC CATGTGTGAA AAAATGAAGA TTCGCGTCAT GGACTGGGAC
    CGCCTTACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC
    CCTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTGGTCA AGCCTTCTCC AGCCACGGAC
    CTGGATGACC ATCTGGGCTT CCTTCCCACC TTCGGGCCCT GCTTCGTCAA TCTCTATGGC
    AGCCCCAGGG AATTCACTGG CTTCCCGGAC CCCTACGCGG AACTCAACAT GGGCAAGGGG
    GAAGGCGTGG CCTACCGAGG TCGGCTTCTG CTCTCCCTGG AGACCAAGCT GGTGGAGCAC
    AGCGGCGAGC AAAGGGTGGA GGACCTCCCT GCTGATGACA TCCTTCGGGT GGAGAAATAC
    CTCCGGAGGC GCAAGTACTC TCTCTTTGCC GCCTTCTACT CGGCCACCAT GCTCCAGGAT
    GTCGACGATG CCATCCAGTT TGAGGTCAGC ATTGGGAACT ATGGCAACAA GTTCGACACG
    ACCTGTCTGC CACTGGCCTC CACCACCCAG TACAGCCGGG CAGTCTTTGA TGGGTGCCAC
    TACTACTACT TGCCCTGGGG CAATGTGAAG CCTGTGGTGG TGCTGTCATC CTACTGGGAA
    GACATCAGCC ACAGAGTCGA GGCTCAGAAC CAGCTGCTCA GGATCGCTGA CCATCTGGAA
    GCTGGCCTGG AGCAAGTCCA CTTGGCTTTA AAGGCGCAGT TCAACACAGA GGATGTGGAC
    TCCCTGTTGG CCCAGCTGAT GGATGAGCTC ATCTCAGACT GCAGCCAGCC CCTGGGCAAT
    GTCCAAGAGA TACCCTCTGC CACCCACCTG GACCGGCACC TGTACAGGCT GCGCACCCGT
    CACCTAGGCC AGATTACTGA GGCGGCCCTG GCTCTGAAAC TCGGGCATGG CAAGCTCCCT
    GCCGCCCTGG AGCAGGCTGA AGACTGGCTC CTGCGTCTCC GTGCCCTGGC TGATGAGCCC
    CAGAACAGCC TGCCCGACAT CATCATCTGG ATGCTGCAGG GAAACAAGCG AGTGGCGTAC
    CAGCGGGTGC CTGCGCACGA GGTCCTCTTC TCCCGGCGGG GCGCCAGCTA CTGTGGCAAG
    AACTGTGGGA AGCTGCAGAC GATATTTCTG AAATATCCCA TGGAGGGGGT GCCCGGAGCC
    CGGATGCCAG TACAAATACG GGTGAAACTC TGGTTTGGGC TTTCTGTGGA TGAGAAGGAG
    TTCAACCAGT TTGCTGAGGG AAAGCTGTCC GTCTTTGCTG AAACTTATGA GAACCAGACC
    AAACTGGCCC TGGTTGGGAA CTGGGGCACA ACAGGCCTCA CCTACCCCAA GTTTTCTGAT
    GTCATGGGCA AGATCAAGCT ACCCAAGGAT AGCTTCCGCC CCTCAGCTGG CTGGACCTGG
    GCCGGAGACT GGTTCGTGTG TCCAGAGAAG ACCCTACTCC ATGACATGGA CGCGGGCCAC
    CTGAGTTTTG TGGAGGAGGT GTTTGAGAAC CAGACCCGGC TCCCTGGAGG CCAGTGGATC
    TACATGAGTG ACAACTACAC AGATGTGAAT GGGGATAAGG TGCTTCCTAA GGATGACATT
    GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT GAAGAGTGGT CCACGGACCT CAATCGGGCC
    GTCGATGAGC AAGGCTGGGA GTACAGTATC ACCATCCCCC CGGATCGGAA GCCAAAGCAC
    TGGGTTCCTG CTGAGAAGAT GTATTACACG CACCGTCGGC GGCGCTGGGT CCGCCTGCGC
    AGAAGGGACC TCAGCCAGAT GGAGGCATTG AAGAGGCACA GACAGGCAGA GGCAGAGGGT
    GAGGGCTGGG AGTACGCCTC GCTCTTGGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACT
    GATGCTTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAAC CGCTGGAGAA GACGGGGCCT
    GCAGCCGTGT TTGCCCTTGA GGGGGCTCTG GGTGGTGTGG TAGATGACAG GAGTGAAGAT
    TCCGTGTCCG TGTCCACCTT GAGCTTTGGA GTGAACAGAC CCACGATTTC CTGCATCTTT
    GATTATGGGA ACCGCTACCA CCTACGCTGC TACATGTACC AGGCTCGGGA CCTGCCCTCG
    ATGGACAAGG ACTCTTTTTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA CCAGAGCCAG
    AAGACAGTGG TGGCGAAGAA CACTCTGAAC CCCACCTGGG ACCAGACGCT TATCTTCTAT
    GAGATTGAGA TCTTTGGTGA GCCAGCCAGT ATCGCTGAGC AGCCGCCCAG CATTGTGGTG
    GAGCTGTATG ACCACGACAC CTACGGTGCA GATGAGTTTA TGGGACGCTG CATTTGTCAG
    CCCAGCCTGG AGCGGACACC CCGGCTGGCC TGGTTCCCAC TGATTAGGGG CAGCCAGTCG
    GCGGGGGAAC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AAAAGCCGGC CATTCACCAT
    ATTCCTGGTT TTGAGGTACA GGATATGTCA GGAATCCTGG AAGAGTCAGA GGACATAGAC
    CTGCCCTACC CGCCACCTCA GAGGGAGGCC AACATCTTCA TGGTCCCCCA GAACATCAAG
    CCTGTTCTCC AGCGTACTGC CATAGAGATC CTGGCGTGGG GCCTGCGGAA CATGAAGAGC
    TACCAGCTAG CCAGCATCTC CTCCCCTAGC CTTGTGGTAG AGTGCGGGGG CCAGACAGTG
    CAGTCCTGTG TCATCAAGAA CCTCCGGAAG AACCCCAACT TCGACATCTG TACCCTTTTC
    ATGGAAGTGA TGCTGCCCAA GGAGGAGGTC TACTGTCCCT CCATCGTGAT CAAGGTCATT
    GATAACCGCC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATCCG TTCCCTGGAG
    AGCTTCATGT GTGACCTGTA CTCCGAGGAG AATCCGTCCC CACAGGGCGG CCCAGATGAT
    GTGAGTTTAC TCAGTCCTGG GGAAGACGTG CTCATTGACA TCGATGACAA GGAGCCCCTC
    ATCCCCATGC AGGAGGAAGA GTTCATCGAC TGGTGGAGCA AATTCTTTGC CTCCACAGGG
    GAGAGGGAAA AGTGCGGCTC ATACTTGGAG AAGGGCTTTG ACACTCTAAA GGTCTATGAC
    ACACAACTGG AGAACGTGGA GGCCTTTGAG GGCCTGTCTG ACTTTTGTAA CACCTTCAAG
    CTCTACCGGG GCAAGACTCA GGAGGAGACA GAGGACCCAT CTGTTATTGG GGAGTTTAAG
    GGCCTCTTCA AAATCTATCC CCTCCCAGAA GACCCGGCTG TTCCCATGCC CCCGAGACAA
    TTCCACCAGC TGGCTGCCCA AGGGCCTCAG GAGTGCCTGG TCCGCATCTA TATCATCCGA
    GCATTTGGCC TTCAGCCCAA GGACCCCAAT GGGAAGTGTG ATCCTTACAT CAAGATCTCC
    ATAGGGAAGA AATCAGTGAG TGACCAGGAT AACTACATTC CCTGTACTCT GGAGCCTGTG
    TTTGGAAAGA TGTTCGAGCT GACCTGCACT CTGCCTCTGG AGAAGGACCT GAAGATCACA
    CTCTATGACT ATGACCTCCT CTCCAAAGAT GAAAAGATTG GGGAGACAGT CATTGACCTG
    GAGGATAGGC TGCTGTCCAA GTTTGGGGCT CGCTGCGGAC TCCCACAGAC GTACTGTGTC
    TCTGGACCCA ACCAGTGGAG GGACCAGCTC CGCCCCTCCC AGCTTCTCCA CCTCTTCTGC
    CAGCAGCGCA GACTCAAGGC TCCTGTGTAC CTCACGGACC ACGTGGTGTT CCAGGATAAA
    GAATACAGCA TTGAAGAAAT AGAGGCTGGC CGAGTCCCGA ACCCACACCT GGGCCCAGTG
    GAAGAGCGTC TGGCCTTGCA TGTCCTCCAG CAGCAGGGCC TGGTCCCTGA GCATGTGGAG
    TCCCGGCCTC TCTACAGCCC CCTGCAGCCA GACATTGAAC AGGGGAAGCT GCAGATGTGG
    ATCGACCTAT TTCCCAAGGC CCTGGGGCGT CCCGGACCTC CCTTCAACAT CACCCCGCGG
    AGAGCCAGAA GGTTTTTCCT GCGTTGTATC ATCTGGAACA CAAAGGACGT GATCCTGGAC
    GACCTCAGCA TCACAGGGGA GAAGATGAGT GATATTTATG TGAAGGGTTG GATGGTTGGC
    TTTGAAGAGC ACAAGCAAAA GACAGATGTG CATTACCGGT CCCTGGGAGG TGAAGGCAAC
    TTTAACTGGA GGTTCATTTT CCCCTTTGAC TACCTGCCAG CCGAGCAAGT CTGCACCGTC
    TCTAAGAAGG ATGCTTTCTG GAGGCTGGAC AAGACTGAGA GCAAAATCCC AGCACGAGTG
    GTATTTCAGA TCTGGGACAA TGACAAGTTC TCCTTTGACG ACTTTCTGGG CTCCTTGCAG
    TTGGATCTCA ACCACATGCC CAAGCCTGCC AAGACAGCTG AGAAGTGCTC CTTGGACCAG
    CTGGATGACA CTTTCCACCC GGAGTGGTTT GTGTCCCTTT TTGAACAGAA AACAGTTAAG
    GGCTGGTGGC CCTGCGTAGC AGAAGAGGGC GAGAAGAAGA TCCTGGCAGG CAAGCTGGAA
    ATGACCTTGG AGATCGTAGC AGAGAGTGAG CATGAGGAGC GGCCTGCTGG CCAGGGCCGG
    GATGAACCCA ACATGAACCC CAAGCTCGAG GACCCAAGGC GCCCTGACAC CTCCTTCCTC
    TGGTTCACCT CTCCATACAA GACTATGAAG TTCATCCTGT GGCGGCGGTT CCGATGTGCC
    ATTATCCTCT TCATCATTCT CTTCATCCTG CTACTATTCT TAGGCATCTT CATCTATGCT
    TTCCCGAACT ACGCTGCCAT GAAGCTGGTG AAGCCCTTCA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003432273.1
    CDS405..6749
    Translation

    Target ORF information:

    RefSeq Version XM_003432225.4
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris dysferlin (DYSF), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003432225.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC
    CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC
    TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA
    CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGGTG GGGGACAGAG CCGGGCTGAG
    ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GATACAAGAT ACTCTGGAAA GAAGTGGCTG
    GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC TGGAGACGAG
    GGAGAGCCAT TACTGGATCA GAGTGGCGTC CCAGGCCCTG GGGCTCCCAC CACCCCGAGG
    AAGCCACCTT CCCATCTGCC CCCCTACCAC CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC
    GCAGCCAGAA AGCTGCTGTC CGACAAACCG CAGGACTTCC AGATCAGAGT GCAAGTGATC
    GAGGGCCGCC AGCTGCCAGG GGTGAATATC AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG
    CAGACCAAGA GGACACGGAT CCACAAGGGA AACAGCCCAC TATTTAACGA GACTCTTTTC
    TTCAATGTGT TTGACTCTCC TTCGGAGCTG TTTGATGAGC CCATCTTTAT CACGGTCGTA
    GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC
    ATCTACAGAG AGCCCCGACA TGCTTATCTG AGGAAGTGGC TGCTACTCTC CAACCCTGAT
    GACTTCTCTG TTGGGGCCAG AGGTTACCTG AAAGCAAGCC TTTATGTGCT GGGGCCCGGA
    GATGAAGCGC CTCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT TGAAAACAAT
    CTGCTCAGGC CAACTGGCGT GGCCCTGCGA GGAGCCCACT TCTGCCTGAA GATCTTCAGA
    GCGGAGGACT TACCACAAAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC
    TTTGACAGCA ACAAGAAGAA TTTGGTAGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA
    ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACT
    CTGCCTGCCA TGTTTCCCTC CATGTGTGAA AAAATGAAGA TTCGCGTCAT GGACTGGGAC
    CGCCTTACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC
    CCTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTGGTCA AGCCTTCTCC AGCCACGGAC
    CTGGATGACC ATCTGGGCTT CCTTCCCACC TTCGGGCCCT GCTTCGTCAA TCTCTATGGC
    AGCCCCAGGG AATTCACTGG CTTCCCGGAC CCCTACGCGG AACTCAACAT GGGCAAGGGG
    GAAGGCGTGG CCTACCGAGG TCGGCTTCTG CTCTCCCTGG AGACCAAGCT GGTGGAGCAC
    AGCGGCGAGC AAAGGGTGGA GGACCTCCCT GCTGATGACA TCCTTCGGGT GGAGAAATAC
    CTCCGGAGGC GCAAGTACTC TCTCTTTGCC GCCTTCTACT CGGCCACCAT GCTCCAGGAT
    GTCGACGATG CCATCCAGTT TGAGGTCAGC ATTGGGAACT ATGGCAACAA GTTCGACACG
    ACCTGTCTGC CACTGGCCTC CACCACCCAG TACAGCCGGG CAGTCTTTGA TGGGTGCCAC
    TACTACTACT TGCCCTGGGG CAATGTGAAG CCTGTGGTGG TGCTGTCATC CTACTGGGAA
    GACATCAGCC ACAGAGTCGA GGCTCAGAAC CAGCTGCTCA GGATCGCTGA CCATCTGGAA
    GCTGGCCTGG AGCAAGTCCA CTTGGCTTTA AAGGCGCAGT TCAACACAGA GGATGTGGAC
    TCCCTGTTGG CCCAGCTGAT GGATGAGCTC ATCTCAGACT GCAGCCAGCC CCTGGGCAAT
    GTCCAAGAGA TACCCTCTGC CACCCACCTG GACCGGCACC TGTACAGGCT GCGCACCCGT
    CACCTAGGCC AGATTACTGA GGCGGCCCTG GCTCTGAAAC TCGGGCATGG CAAGCTCCCT
    GCCGCCCTGG AGCAGGCTGA AGACTGGCTC CTGCGTCTCC GTGCCCTGGC TGATGAGCCC
    CAGAACAGCC TGCCCGACAT CATCATCTGG ATGCTGCAGG GAAACAAGCG AGTGGCGTAC
    CAGCGGGTGC CTGCGCACGA GGTCCTCTTC TCCCGGCGGG GCGCCAGCTA CTGTGGCAAG
    AACTGTGGGA AGCTGCAGAC GATATTTCTG AAATATCCCA TGGAGGGGGT GCCCGGAGCC
    CGGATGCCAG TACAAATACG GGTGAAACTC TGGTTTGGGC TTTCTGTGGA TGAGAAGGAG
    TTCAACCAGT TTGCTGAGGG AAAGCTGTCC GTCTTTGCTG AAACTTATGA GAACCAGACC
    AAACTGGCCC TGGTTGGGAA CTGGGGCACA ACAGGCCTCA CCTACCCCAA GTTTTCTGAT
    GTCATGGGCA AGATCAAGCT ACCCAAGGAT AGCTTCCGCC CCTCAGCTGG CTGGACCTGG
    GCCGGAGACT GGTTCGTGTG TCCAGAGAAG ACCCTACTCC ATGACATGGA CGCGGGCCAC
    CTGAGTTTTG TGGAGGAGGT GTTTGAGAAC CAGACCCGGC TCCCTGGAGG CCAGTGGATC
    TACATGAGTG ACAACTACAC AGATGTGAAT GGGGATAAGG TGCTTCCTAA GGATGACATT
    GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT GAAGAGTGGT CCACGGACCT CAATCGGGCC
    GTCGATGAGC AAGGCTGGGA GTACAGTATC ACCATCCCCC CGGATCGGAA GCCAAAGCAC
    TGGGTTCCTG CTGAGAAGAT GTATTACACG CACCGTCGGC GGCGCTGGGT CCGCCTGCGC
    AGAAGGGACC TCAGCCAGAT GGAGGCATTG AAGAGGCACA GACAGGCAGA GGCAGAGGGT
    GAGGGCTGGG AGTACGCCTC GCTCTTGGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACT
    GATGCTTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAAC CGCTGGAGAA GACGGGGCCT
    GCAGCCGTGT TTGCCCTTGA GGGGGCTCTG GGTGGTGTGG TAGATGACAG GAGTGAAGAT
    TCCGTGTCCG TGTCCACCTT GAGCTTTGGA GTGAACAGAC CCACGATTTC CTGCATCTTT
    GATTATGGGA ACCGCTACCA CCTACGCTGC TACATGTACC AGGCTCGGGA CCTGCCCTCG
    ATGGACAAGG ACTCTTTTTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA CCAGAGCCAG
    AAGACAGTGG TGGCGAAGAA CACTCTGAAC CCCACCTGGG ACCAGACGCT TATCTTCTAT
    GAGATTGAGA TCTTTGGTGA GCCAGCCAGT ATCGCTGAGC AGCCGCCCAG CATTGTGGTG
    GAGCTGTATG ACCACGACAC CTACGGTGCA GATGAGTTTA TGGGACGCTG CATTTGTCAG
    CCCAGCCTGG AGCGGACACC CCGGCTGGCC TGGTTCCCAC TGATTAGGGG CAGCCAGTCG
    GCGGGGGAAC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AAAAGCCGGC CATTCACCAT
    ATTCCTGGTT TTGAGGTACA GGATATGTCA GGAATCCTGG AAGAGTCAGA GGACATAGAC
    CTGCCCTACC CGCCACCTCA GAGGGAGGCC AACATCTTCA TGGTCCCCCA GAACATCAAG
    CCTGTTCTCC AGCGTACTGC CATAGAGATC CTGGCGTGGG GCCTGCGGAA CATGAAGAGC
    TACCAGCTAG CCAGCATCTC CTCCCCTAGC CTTGTGGTAG AGTGCGGGGG CCAGACAGTG
    CAGTCCTGTG TCATCAAGAA CCTCCGGAAG AACCCCAACT TCGACATCTG TACCCTTTTC
    ATGGAAGTGA TGCTGCCCAA GGAGGAGGTC TACTGTCCCT CCATCGTGAT CAAGGTCATT
    GATAACCGCC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATCCG TTCCCTGGAG
    AGCTTCATGT GTGACCTGTA CTCCGAGGAG AATCCGTCCC CACAGGGCGG CCCAGATGAT
    GTGAGTTTAC TCAGTCCTGG GGAAGACGTG CTCATTGACA TCGATGACAA GGAGCCCCTC
    ATCCCCATGC AGGAGGAAGA GTTCATCGAC TGGTGGAGCA AATTCTTTGC CTCCACAGGG
    GAGAGGGAAA AGTGCGGCTC ATACTTGGAG AAGGGCTTTG ACACTCTAAA GGTCTATGAC
    ACACAACTGG AGAACGTGGA GGCCTTTGAG GGCCTGTCTG ACTTTTGTAA CACCTTCAAG
    CTCTACCGGG GCAAGACTCA GGAGGAGACA GAGGACCCAT CTGTTATTGG GGAGTTTAAG
    GGCCTCTTCA AAATCTATCC CCTCCCAGAA GACCCGGCTG TTCCCATGCC CCCGAGACAA
    TTCCACCAGC TGGCTGCCCA AGGGCCTCAG GAGTGCCTGG TCCGCATCTA TATCATCCGA
    GCATTTGGCC TTCAGCCCAA GGACCCCAAT GGGAAGTGTG ATCCTTACAT CAAGATCTCC
    ATAGGGAAGA AATCAGTGAG TGACCAGGAT AACTACATTC CCTGTACTCT GGAGCCTGTG
    TTTGGAAAGA TGTTCGAGCT GACCTGCACT CTGCCTCTGG AGAAGGACCT GAAGATCACA
    CTCTATGACT ATGACCTCCT CTCCAAAGAT GAAAAGATTG GGGAGACAGT CATTGACCTG
    GAGGATAGGC TGCTGTCCAA GTTTGGGGCT CGCTGCGGAC TCCCACAGAC GTACTGTGTC
    TCTGGACCCA ACCAGTGGAG GGACCAGCTC CGCCCCTCCC AGCTTCTCCA CCTCTTCTGC
    CAGCAGCGCA GACTCAAGGC TCCTGTGTAC CTCACGGACC ACGTGGTGTT CCAGGATAAA
    GAATACAGCA TTGAAGAAAT AGAGGCTGGC CGAGTCCCGA ACCCACACCT GGGCCCAGTG
    GAAGAGCGTC TGGCCTTGCA TGTCCTCCAG CAGCAGGGCC TGGTCCCTGA GCATGTGGAG
    TCCCGGCCTC TCTACAGCCC CCTGCAGCCA GACATTGAAC AGGGGAAGCT GCAGATGTGG
    ATCGACCTAT TTCCCAAGGC CCTGGGGCGT CCCGGACCTC CCTTCAACAT CACCCCGCGG
    AGAGCCAGAA GGTTTTTCCT GCGTTGTATC ATCTGGAACA CAAAGGACGT GATCCTGGAC
    GACCTCAGCA TCACAGGGGA GAAGATGAGT GATATTTATG TGAAGGGTTG GATGGTTGGC
    TTTGAAGAGC ACAAGCAAAA GACAGATGTG CATTACCGGT CCCTGGGAGG TGAAGGCAAC
    TTTAACTGGA GGTTCATTTT CCCCTTTGAC TACCTGCCAG CCGAGCAAGT CTGCACCGTC
    TCTAAGAAGG ATGCTTTCTG GAGGCTGGAC AAGACTGAGA GCAAAATCCC AGCACGAGTG
    GTATTTCAGA TCTGGGACAA TGACAAGTTC TCCTTTGACG ACTTTCTGGG CTCCTTGCAG
    TTGGATCTCA ACCACATGCC CAAGCCTGCC AAGACAGCTG AGAAGTGCTC CTTGGACCAG
    CTGGATGACA CTTTCCACCC GGAGTGGTTT GTGTCCCTTT TTGAACAGAA AACAGTTAAG
    GGCTGGTGGC CCTGCGTAGC AGAAGAGGGC GAGAAGAAGA TCCTGGCAGG CAAGCTGGAA
    ATGACCTTGG AGATCGTAGC AGAGAGTGAG CATGAGGAGC GGCCTGCTGG CCAGGGCCGG
    GATGAACCCA ACATGAACCC CAAGCTCGAG GACCCAAGGC GCCCTGACAC CTCCTTCCTC
    TGGTTCACCT CTCCATACAA GACTATGAAG TTCATCCTGT GGCGGCGGTT CCGATGTGCC
    ATTATCCTCT TCATCATTCT CTTCATCCTG CTACTATTCT TAGGCATCTT CATCTATGCT
    TTCCCGAACT ACGCTGCCAT GAAGCTGGTG AAGCCCTTCA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe19209
    Clone ID Related Accession (Same CDS sequence) XM_003432225.4 , XM_003432225.3
    Accession Version XM_003432225.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6345bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product dysferlin isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003432225.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC
    CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC
    TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA
    CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGGTG GGGGACAGAG CCGGGCTGAG
    ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GATACAAGAT ACTCTGGAAA GAAGTGGCTG
    GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC TGGAGACGAG
    GGAGAGCCAT TACTGGATCA GAGTGGCGTC CCAGGCCCTG GGGCTCCCAC CACCCCGAGG
    AAGCCACCTT CCCATCTGCC CCCCTACCAC CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC
    GCAGCCAGAA AGCTGCTGTC CGACAAACCG CAGGACTTCC AGATCAGAGT GCAAGTGATC
    GAGGGCCGCC AGCTGCCAGG GGTGAATATC AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG
    CAGACCAAGA GGACACGGAT CCACAAGGGA AACAGCCCAC TATTTAACGA GACTCTTTTC
    TTCAATGTGT TTGACTCTCC TTCGGAGCTG TTTGATGAGC CCATCTTTAT CACGGTCGTA
    GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC
    ATCTACAGAG AGCCCCGACA TGCTTATCTG AGGAAGTGGC TGCTACTCTC CAACCCTGAT
    GACTTCTCTG TTGGGGCCAG AGGTTACCTG AAAGCAAGCC TTTATGTGCT GGGGCCCGGA
    GATGAAGCGC CTCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT TGAAAACAAT
    CTGCTCAGGC CAACTGGCGT GGCCCTGCGA GGAGCCCACT TCTGCCTGAA GATCTTCAGA
    GCGGAGGACT TACCACAAAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC
    TTTGACAGCA ACAAGAAGAA TTTGGTAGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA
    ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACT
    CTGCCTGCCA TGTTTCCCTC CATGTGTGAA AAAATGAAGA TTCGCGTCAT GGACTGGGAC
    CGCCTTACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC
    CCTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTGGTCA AGCCTTCTCC AGCCACGGAC
    CTGGATGACC ATCTGGGCTT CCTTCCCACC TTCGGGCCCT GCTTCGTCAA TCTCTATGGC
    AGCCCCAGGG AATTCACTGG CTTCCCGGAC CCCTACGCGG AACTCAACAT GGGCAAGGGG
    GAAGGCGTGG CCTACCGAGG TCGGCTTCTG CTCTCCCTGG AGACCAAGCT GGTGGAGCAC
    AGCGGCGAGC AAAGGGTGGA GGACCTCCCT GCTGATGACA TCCTTCGGGT GGAGAAATAC
    CTCCGGAGGC GCAAGTACTC TCTCTTTGCC GCCTTCTACT CGGCCACCAT GCTCCAGGAT
    GTCGACGATG CCATCCAGTT TGAGGTCAGC ATTGGGAACT ATGGCAACAA GTTCGACACG
    ACCTGTCTGC CACTGGCCTC CACCACCCAG TACAGCCGGG CAGTCTTTGA TGGGTGCCAC
    TACTACTACT TGCCCTGGGG CAATGTGAAG CCTGTGGTGG TGCTGTCATC CTACTGGGAA
    GACATCAGCC ACAGAGTCGA GGCTCAGAAC CAGCTGCTCA GGATCGCTGA CCATCTGGAA
    GCTGGCCTGG AGCAAGTCCA CTTGGCTTTA AAGGCGCAGT TCAACACAGA GGATGTGGAC
    TCCCTGTTGG CCCAGCTGAT GGATGAGCTC ATCTCAGACT GCAGCCAGCC CCTGGGCAAT
    GTCCAAGAGA TACCCTCTGC CACCCACCTG GACCGGCACC TGTACAGGCT GCGCACCCGT
    CACCTAGGCC AGATTACTGA GGCGGCCCTG GCTCTGAAAC TCGGGCATGG CAAGCTCCCT
    GCCGCCCTGG AGCAGGCTGA AGACTGGCTC CTGCGTCTCC GTGCCCTGGC TGATGAGCCC
    CAGAACAGCC TGCCCGACAT CATCATCTGG ATGCTGCAGG GAAACAAGCG AGTGGCGTAC
    CAGCGGGTGC CTGCGCACGA GGTCCTCTTC TCCCGGCGGG GCGCCAGCTA CTGTGGCAAG
    AACTGTGGGA AGCTGCAGAC GATATTTCTG AAATATCCCA TGGAGGGGGT GCCCGGAGCC
    CGGATGCCAG TACAAATACG GGTGAAACTC TGGTTTGGGC TTTCTGTGGA TGAGAAGGAG
    TTCAACCAGT TTGCTGAGGG AAAGCTGTCC GTCTTTGCTG AAACTTATGA GAACCAGACC
    AAACTGGCCC TGGTTGGGAA CTGGGGCACA ACAGGCCTCA CCTACCCCAA GTTTTCTGAT
    GTCATGGGCA AGATCAAGCT ACCCAAGGAT AGCTTCCGCC CCTCAGCTGG CTGGACCTGG
    GCCGGAGACT GGTTCGTGTG TCCAGAGAAG ACCCTACTCC ATGACATGGA CGCGGGCCAC
    CTGAGTTTTG TGGAGGAGGT GTTTGAGAAC CAGACCCGGC TCCCTGGAGG CCAGTGGATC
    TACATGAGTG ACAACTACAC AGATGTGAAT GGGGATAAGG TGCTTCCTAA GGATGACATT
    GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT GAAGAGTGGT CCACGGACCT CAATCGGGCC
    GTCGATGAGC AAGGCTGGGA GTACAGTATC ACCATCCCCC CGGATCGGAA GCCAAAGCAC
    TGGGTTCCTG CTGAGAAGAT GTATTACACG CACCGTCGGC GGCGCTGGGT CCGCCTGCGC
    AGAAGGGACC TCAGCCAGAT GGAGGCATTG AAGAGGCACA GACAGGCAGA GGCAGAGGGT
    GAGGGCTGGG AGTACGCCTC GCTCTTGGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACT
    GATGCTTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAAC CGCTGGAGAA GACGGGGCCT
    GCAGCCGTGT TTGCCCTTGA GGGGGCTCTG GGTGGTGTGG TAGATGACAG GAGTGAAGAT
    TCCGTGTCCG TGTCCACCTT GAGCTTTGGA GTGAACAGAC CCACGATTTC CTGCATCTTT
    GATTATGGGA ACCGCTACCA CCTACGCTGC TACATGTACC AGGCTCGGGA CCTGCCCTCG
    ATGGACAAGG ACTCTTTTTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA CCAGAGCCAG
    AAGACAGTGG TGGCGAAGAA CACTCTGAAC CCCACCTGGG ACCAGACGCT TATCTTCTAT
    GAGATTGAGA TCTTTGGTGA GCCAGCCAGT ATCGCTGAGC AGCCGCCCAG CATTGTGGTG
    GAGCTGTATG ACCACGACAC CTACGGTGCA GATGAGTTTA TGGGACGCTG CATTTGTCAG
    CCCAGCCTGG AGCGGACACC CCGGCTGGCC TGGTTCCCAC TGATTAGGGG CAGCCAGTCG
    GCGGGGGAAC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AAAAGCCGGC CATTCACCAT
    ATTCCTGGTT TTGAGGTACA GGATATGTCA GGAATCCTGG AAGAGTCAGA GGACATAGAC
    CTGCCCTACC CGCCACCTCA GAGGGAGGCC AACATCTTCA TGGTCCCCCA GAACATCAAG
    CCTGTTCTCC AGCGTACTGC CATAGAGATC CTGGCGTGGG GCCTGCGGAA CATGAAGAGC
    TACCAGCTAG CCAGCATCTC CTCCCCTAGC CTTGTGGTAG AGTGCGGGGG CCAGACAGTG
    CAGTCCTGTG TCATCAAGAA CCTCCGGAAG AACCCCAACT TCGACATCTG TACCCTTTTC
    ATGGAAGTGA TGCTGCCCAA GGAGGAGGTC TACTGTCCCT CCATCGTGAT CAAGGTCATT
    GATAACCGCC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATCCG TTCCCTGGAG
    AGCTTCATGT GTGACCTGTA CTCCGAGGAG AATCCGTCCC CACAGGGCGG CCCAGATGAT
    GTGAGTTTAC TCAGTCCTGG GGAAGACGTG CTCATTGACA TCGATGACAA GGAGCCCCTC
    ATCCCCATGC AGGAGGAAGA GTTCATCGAC TGGTGGAGCA AATTCTTTGC CTCCACAGGG
    GAGAGGGAAA AGTGCGGCTC ATACTTGGAG AAGGGCTTTG ACACTCTAAA GGTCTATGAC
    ACACAACTGG AGAACGTGGA GGCCTTTGAG GGCCTGTCTG ACTTTTGTAA CACCTTCAAG
    CTCTACCGGG GCAAGACTCA GGAGGAGACA GAGGACCCAT CTGTTATTGG GGAGTTTAAG
    GGCCTCTTCA AAATCTATCC CCTCCCAGAA GACCCGGCTG TTCCCATGCC CCCGAGACAA
    TTCCACCAGC TGGCTGCCCA AGGGCCTCAG GAGTGCCTGG TCCGCATCTA TATCATCCGA
    GCATTTGGCC TTCAGCCCAA GGACCCCAAT GGGAAGTGTG ATCCTTACAT CAAGATCTCC
    ATAGGGAAGA AATCAGTGAG TGACCAGGAT AACTACATTC CCTGTACTCT GGAGCCTGTG
    TTTGGAAAGA TGTTCGAGCT GACCTGCACT CTGCCTCTGG AGAAGGACCT GAAGATCACA
    CTCTATGACT ATGACCTCCT CTCCAAAGAT GAAAAGATTG GGGAGACAGT CATTGACCTG
    GAGGATAGGC TGCTGTCCAA GTTTGGGGCT CGCTGCGGAC TCCCACAGAC GTACTGTGTC
    TCTGGACCCA ACCAGTGGAG GGACCAGCTC CGCCCCTCCC AGCTTCTCCA CCTCTTCTGC
    CAGCAGCGCA GACTCAAGGC TCCTGTGTAC CTCACGGACC ACGTGGTGTT CCAGGATAAA
    GAATACAGCA TTGAAGAAAT AGAGGCTGGC CGAGTCCCGA ACCCACACCT GGGCCCAGTG
    GAAGAGCGTC TGGCCTTGCA TGTCCTCCAG CAGCAGGGCC TGGTCCCTGA GCATGTGGAG
    TCCCGGCCTC TCTACAGCCC CCTGCAGCCA GACATTGAAC AGGGGAAGCT GCAGATGTGG
    ATCGACCTAT TTCCCAAGGC CCTGGGGCGT CCCGGACCTC CCTTCAACAT CACCCCGCGG
    AGAGCCAGAA GGTTTTTCCT GCGTTGTATC ATCTGGAACA CAAAGGACGT GATCCTGGAC
    GACCTCAGCA TCACAGGGGA GAAGATGAGT GATATTTATG TGAAGGGTTG GATGGTTGGC
    TTTGAAGAGC ACAAGCAAAA GACAGATGTG CATTACCGGT CCCTGGGAGG TGAAGGCAAC
    TTTAACTGGA GGTTCATTTT CCCCTTTGAC TACCTGCCAG CCGAGCAAGT CTGCACCGTC
    TCTAAGAAGG ATGCTTTCTG GAGGCTGGAC AAGACTGAGA GCAAAATCCC AGCACGAGTG
    GTATTTCAGA TCTGGGACAA TGACAAGTTC TCCTTTGACG ACTTTCTGGG CTCCTTGCAG
    TTGGATCTCA ACCACATGCC CAAGCCTGCC AAGACAGCTG AGAAGTGCTC CTTGGACCAG
    CTGGATGACA CTTTCCACCC GGAGTGGTTT GTGTCCCTTT TTGAACAGAA AACAGTTAAG
    GGCTGGTGGC CCTGCGTAGC AGAAGAGGGC GAGAAGAAGA TCCTGGCAGG CAAGCTGGAA
    ATGACCTTGG AGATCGTAGC AGAGAGTGAG CATGAGGAGC GGCCTGCTGG CCAGGGCCGG
    GATGAACCCA ACATGAACCC CAAGCTCGAG GACCCAAGGC GCCCTGACAC CTCCTTCCTC
    TGGTTCACCT CTCCATACAA GACTATGAAG TTCATCCTGT GGCGGCGGTT CCGATGTGCC
    ATTATCCTCT TCATCATTCT CTTCATCCTG CTACTATTCT TAGGCATCTT CATCTATGCT
    TTCCCGAACT ACGCTGCCAT GAAGCTGGTG AAGCCCTTCA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003432273.1
    CDS439..6783
    Translation

    Target ORF information:

    RefSeq Version XM_003432225.3
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris dysferlin (DYSF), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003432225.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC
    CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC
    TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA
    CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGGTG GGGGACAGAG CCGGGCTGAG
    ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GATACAAGAT ACTCTGGAAA GAAGTGGCTG
    GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC TGGAGACGAG
    GGAGAGCCAT TACTGGATCA GAGTGGCGTC CCAGGCCCTG GGGCTCCCAC CACCCCGAGG
    AAGCCACCTT CCCATCTGCC CCCCTACCAC CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC
    GCAGCCAGAA AGCTGCTGTC CGACAAACCG CAGGACTTCC AGATCAGAGT GCAAGTGATC
    GAGGGCCGCC AGCTGCCAGG GGTGAATATC AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG
    CAGACCAAGA GGACACGGAT CCACAAGGGA AACAGCCCAC TATTTAACGA GACTCTTTTC
    TTCAATGTGT TTGACTCTCC TTCGGAGCTG TTTGATGAGC CCATCTTTAT CACGGTCGTA
    GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC
    ATCTACAGAG AGCCCCGACA TGCTTATCTG AGGAAGTGGC TGCTACTCTC CAACCCTGAT
    GACTTCTCTG TTGGGGCCAG AGGTTACCTG AAAGCAAGCC TTTATGTGCT GGGGCCCGGA
    GATGAAGCGC CTCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT TGAAAACAAT
    CTGCTCAGGC CAACTGGCGT GGCCCTGCGA GGAGCCCACT TCTGCCTGAA GATCTTCAGA
    GCGGAGGACT TACCACAAAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC
    TTTGACAGCA ACAAGAAGAA TTTGGTAGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA
    ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACT
    CTGCCTGCCA TGTTTCCCTC CATGTGTGAA AAAATGAAGA TTCGCGTCAT GGACTGGGAC
    CGCCTTACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC
    CCTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTGGTCA AGCCTTCTCC AGCCACGGAC
    CTGGATGACC ATCTGGGCTT CCTTCCCACC TTCGGGCCCT GCTTCGTCAA TCTCTATGGC
    AGCCCCAGGG AATTCACTGG CTTCCCGGAC CCCTACGCGG AACTCAACAT GGGCAAGGGG
    GAAGGCGTGG CCTACCGAGG TCGGCTTCTG CTCTCCCTGG AGACCAAGCT GGTGGAGCAC
    AGCGGCGAGC AAAGGGTGGA GGACCTCCCT GCTGATGACA TCCTTCGGGT GGAGAAATAC
    CTCCGGAGGC GCAAGTACTC TCTCTTTGCC GCCTTCTACT CGGCCACCAT GCTCCAGGAT
    GTCGACGATG CCATCCAGTT TGAGGTCAGC ATTGGGAACT ATGGCAACAA GTTCGACACG
    ACCTGTCTGC CACTGGCCTC CACCACCCAG TACAGCCGGG CAGTCTTTGA TGGGTGCCAC
    TACTACTACT TGCCCTGGGG CAATGTGAAG CCTGTGGTGG TGCTGTCATC CTACTGGGAA
    GACATCAGCC ACAGAGTCGA GGCTCAGAAC CAGCTGCTCA GGATCGCTGA CCATCTGGAA
    GCTGGCCTGG AGCAAGTCCA CTTGGCTTTA AAGGCGCAGT TCAACACAGA GGATGTGGAC
    TCCCTGTTGG CCCAGCTGAT GGATGAGCTC ATCTCAGACT GCAGCCAGCC CCTGGGCAAT
    GTCCAAGAGA TACCCTCTGC CACCCACCTG GACCGGCACC TGTACAGGCT GCGCACCCGT
    CACCTAGGCC AGATTACTGA GGCGGCCCTG GCTCTGAAAC TCGGGCATGG CAAGCTCCCT
    GCCGCCCTGG AGCAGGCTGA AGACTGGCTC CTGCGTCTCC GTGCCCTGGC TGATGAGCCC
    CAGAACAGCC TGCCCGACAT CATCATCTGG ATGCTGCAGG GAAACAAGCG AGTGGCGTAC
    CAGCGGGTGC CTGCGCACGA GGTCCTCTTC TCCCGGCGGG GCGCCAGCTA CTGTGGCAAG
    AACTGTGGGA AGCTGCAGAC GATATTTCTG AAATATCCCA TGGAGGGGGT GCCCGGAGCC
    CGGATGCCAG TACAAATACG GGTGAAACTC TGGTTTGGGC TTTCTGTGGA TGAGAAGGAG
    TTCAACCAGT TTGCTGAGGG AAAGCTGTCC GTCTTTGCTG AAACTTATGA GAACCAGACC
    AAACTGGCCC TGGTTGGGAA CTGGGGCACA ACAGGCCTCA CCTACCCCAA GTTTTCTGAT
    GTCATGGGCA AGATCAAGCT ACCCAAGGAT AGCTTCCGCC CCTCAGCTGG CTGGACCTGG
    GCCGGAGACT GGTTCGTGTG TCCAGAGAAG ACCCTACTCC ATGACATGGA CGCGGGCCAC
    CTGAGTTTTG TGGAGGAGGT GTTTGAGAAC CAGACCCGGC TCCCTGGAGG CCAGTGGATC
    TACATGAGTG ACAACTACAC AGATGTGAAT GGGGATAAGG TGCTTCCTAA GGATGACATT
    GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT GAAGAGTGGT CCACGGACCT CAATCGGGCC
    GTCGATGAGC AAGGCTGGGA GTACAGTATC ACCATCCCCC CGGATCGGAA GCCAAAGCAC
    TGGGTTCCTG CTGAGAAGAT GTATTACACG CACCGTCGGC GGCGCTGGGT CCGCCTGCGC
    AGAAGGGACC TCAGCCAGAT GGAGGCATTG AAGAGGCACA GACAGGCAGA GGCAGAGGGT
    GAGGGCTGGG AGTACGCCTC GCTCTTGGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACT
    GATGCTTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAAC CGCTGGAGAA GACGGGGCCT
    GCAGCCGTGT TTGCCCTTGA GGGGGCTCTG GGTGGTGTGG TAGATGACAG GAGTGAAGAT
    TCCGTGTCCG TGTCCACCTT GAGCTTTGGA GTGAACAGAC CCACGATTTC CTGCATCTTT
    GATTATGGGA ACCGCTACCA CCTACGCTGC TACATGTACC AGGCTCGGGA CCTGCCCTCG
    ATGGACAAGG ACTCTTTTTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA CCAGAGCCAG
    AAGACAGTGG TGGCGAAGAA CACTCTGAAC CCCACCTGGG ACCAGACGCT TATCTTCTAT
    GAGATTGAGA TCTTTGGTGA GCCAGCCAGT ATCGCTGAGC AGCCGCCCAG CATTGTGGTG
    GAGCTGTATG ACCACGACAC CTACGGTGCA GATGAGTTTA TGGGACGCTG CATTTGTCAG
    CCCAGCCTGG AGCGGACACC CCGGCTGGCC TGGTTCCCAC TGATTAGGGG CAGCCAGTCG
    GCGGGGGAAC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AAAAGCCGGC CATTCACCAT
    ATTCCTGGTT TTGAGGTACA GGATATGTCA GGAATCCTGG AAGAGTCAGA GGACATAGAC
    CTGCCCTACC CGCCACCTCA GAGGGAGGCC AACATCTTCA TGGTCCCCCA GAACATCAAG
    CCTGTTCTCC AGCGTACTGC CATAGAGATC CTGGCGTGGG GCCTGCGGAA CATGAAGAGC
    TACCAGCTAG CCAGCATCTC CTCCCCTAGC CTTGTGGTAG AGTGCGGGGG CCAGACAGTG
    CAGTCCTGTG TCATCAAGAA CCTCCGGAAG AACCCCAACT TCGACATCTG TACCCTTTTC
    ATGGAAGTGA TGCTGCCCAA GGAGGAGGTC TACTGTCCCT CCATCGTGAT CAAGGTCATT
    GATAACCGCC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATCCG TTCCCTGGAG
    AGCTTCATGT GTGACCTGTA CTCCGAGGAG AATCCGTCCC CACAGGGCGG CCCAGATGAT
    GTGAGTTTAC TCAGTCCTGG GGAAGACGTG CTCATTGACA TCGATGACAA GGAGCCCCTC
    ATCCCCATGC AGGAGGAAGA GTTCATCGAC TGGTGGAGCA AATTCTTTGC CTCCACAGGG
    GAGAGGGAAA AGTGCGGCTC ATACTTGGAG AAGGGCTTTG ACACTCTAAA GGTCTATGAC
    ACACAACTGG AGAACGTGGA GGCCTTTGAG GGCCTGTCTG ACTTTTGTAA CACCTTCAAG
    CTCTACCGGG GCAAGACTCA GGAGGAGACA GAGGACCCAT CTGTTATTGG GGAGTTTAAG
    GGCCTCTTCA AAATCTATCC CCTCCCAGAA GACCCGGCTG TTCCCATGCC CCCGAGACAA
    TTCCACCAGC TGGCTGCCCA AGGGCCTCAG GAGTGCCTGG TCCGCATCTA TATCATCCGA
    GCATTTGGCC TTCAGCCCAA GGACCCCAAT GGGAAGTGTG ATCCTTACAT CAAGATCTCC
    ATAGGGAAGA AATCAGTGAG TGACCAGGAT AACTACATTC CCTGTACTCT GGAGCCTGTG
    TTTGGAAAGA TGTTCGAGCT GACCTGCACT CTGCCTCTGG AGAAGGACCT GAAGATCACA
    CTCTATGACT ATGACCTCCT CTCCAAAGAT GAAAAGATTG GGGAGACAGT CATTGACCTG
    GAGGATAGGC TGCTGTCCAA GTTTGGGGCT CGCTGCGGAC TCCCACAGAC GTACTGTGTC
    TCTGGACCCA ACCAGTGGAG GGACCAGCTC CGCCCCTCCC AGCTTCTCCA CCTCTTCTGC
    CAGCAGCGCA GACTCAAGGC TCCTGTGTAC CTCACGGACC ACGTGGTGTT CCAGGATAAA
    GAATACAGCA TTGAAGAAAT AGAGGCTGGC CGAGTCCCGA ACCCACACCT GGGCCCAGTG
    GAAGAGCGTC TGGCCTTGCA TGTCCTCCAG CAGCAGGGCC TGGTCCCTGA GCATGTGGAG
    TCCCGGCCTC TCTACAGCCC CCTGCAGCCA GACATTGAAC AGGGGAAGCT GCAGATGTGG
    ATCGACCTAT TTCCCAAGGC CCTGGGGCGT CCCGGACCTC CCTTCAACAT CACCCCGCGG
    AGAGCCAGAA GGTTTTTCCT GCGTTGTATC ATCTGGAACA CAAAGGACGT GATCCTGGAC
    GACCTCAGCA TCACAGGGGA GAAGATGAGT GATATTTATG TGAAGGGTTG GATGGTTGGC
    TTTGAAGAGC ACAAGCAAAA GACAGATGTG CATTACCGGT CCCTGGGAGG TGAAGGCAAC
    TTTAACTGGA GGTTCATTTT CCCCTTTGAC TACCTGCCAG CCGAGCAAGT CTGCACCGTC
    TCTAAGAAGG ATGCTTTCTG GAGGCTGGAC AAGACTGAGA GCAAAATCCC AGCACGAGTG
    GTATTTCAGA TCTGGGACAA TGACAAGTTC TCCTTTGACG ACTTTCTGGG CTCCTTGCAG
    TTGGATCTCA ACCACATGCC CAAGCCTGCC AAGACAGCTG AGAAGTGCTC CTTGGACCAG
    CTGGATGACA CTTTCCACCC GGAGTGGTTT GTGTCCCTTT TTGAACAGAA AACAGTTAAG
    GGCTGGTGGC CCTGCGTAGC AGAAGAGGGC GAGAAGAAGA TCCTGGCAGG CAAGCTGGAA
    ATGACCTTGG AGATCGTAGC AGAGAGTGAG CATGAGGAGC GGCCTGCTGG CCAGGGCCGG
    GATGAACCCA ACATGAACCC CAAGCTCGAG GACCCAAGGC GCCCTGACAC CTCCTTCCTC
    TGGTTCACCT CTCCATACAA GACTATGAAG TTCATCCTGT GGCGGCGGTT CCGATGTGCC
    ATTATCCTCT TCATCATTCT CTTCATCCTG CTACTATTCT TAGGCATCTT CATCTATGCT
    TTCCCGAACT ACGCTGCCAT GAAGCTGGTG AAGCCCTTCA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe19210
    Clone ID Related Accession (Same CDS sequence) XM_003432226.4 , XM_003432226.3
    Accession Version XM_003432226.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6318bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product dysferlin isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003432226.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG ACACAGGAGG AGAGGAGGAC
    ACTGAGGACC AGGGGCTCAC TGGAGACGAG GGAGAGCCAT TACTGGATCA GAGTGGCGTC
    CCAGGCCCTG GGGCTCCCAC CACCCCGAGG AAGCCACCTT CCCATCTGCC CCCCTACCAC
    CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC GCAGCCAGAA AGCTGCTGTC CGACAAACCG
    CAGGACTTCC AGATCAGAGT GCAAGTGATC GAGGGCCGCC AGCTGCCAGG GGTGAATATC
    AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG CAGACCAAGA GGACACGGAT CCACAAGGGA
    AACAGCCCAC TATTTAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TTCGGAGCTG
    TTTGATGAGC CCATCTTTAT CACGGTCGTA GACTCCCGTT CTCTCAGGAC AGATGCTCTC
    ATTGGGGAAT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA TGCTTATCTG
    AGGAAGTGGC TGCTACTCTC CAACCCTGAT GACTTCTCTG TTGGGGCCAG AGGTTACCTG
    AAAGCAAGCC TTTATGTGCT GGGGCCCGGA GATGAAGCGC CTCTGGAGAG AAAGGACCCC
    TCTGAAGACA AGGAGGACAT TGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTGCGA
    GGAGCCCACT TCTGCCTGAA GATCTTCAGA GCGGAGGACT TACCACAAAT GGATGATGCC
    GTGATGGACA ATGTGAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA TTTGGTAGAT
    CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA
    GCCAACCCTC AGTGGAACCA GAGCATCACT CTGCCTGCCA TGTTTCCCTC CATGTGTGAA
    AAAATGAAGA TTCGCGTCAT GGACTGGGAC CGCCTTACCC ACAATGACAT CGTGGCCACC
    ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGAAGA GGAGCCTGCA
    GGTGTGGTCA AGCCTTCTCC AGCCACGGAC CTGGATGACC ATCTGGGCTT CCTTCCCACC
    TTCGGGCCCT GCTTCGTCAA TCTCTATGGC AGCCCCAGGG AATTCACTGG CTTCCCGGAC
    CCCTACGCGG AACTCAACAT GGGCAAGGGG GAAGGCGTGG CCTACCGAGG TCGGCTTCTG
    CTCTCCCTGG AGACCAAGCT GGTGGAGCAC AGCGGCGAGC AAAGGGTGGA GGACCTCCCT
    GCTGATGACA TCCTTCGGGT GGAGAAATAC CTCCGGAGGC GCAAGTACTC TCTCTTTGCC
    GCCTTCTACT CGGCCACCAT GCTCCAGGAT GTCGACGATG CCATCCAGTT TGAGGTCAGC
    ATTGGGAACT ATGGCAACAA GTTCGACACG ACCTGTCTGC CACTGGCCTC CACCACCCAG
    TACAGCCGGG CAGTCTTTGA TGGGTGCCAC TACTACTACT TGCCCTGGGG CAATGTGAAG
    CCTGTGGTGG TGCTGTCATC CTACTGGGAA GACATCAGCC ACAGAGTCGA GGCTCAGAAC
    CAGCTGCTCA GGATCGCTGA CCATCTGGAA GCTGGCCTGG AGCAAGTCCA CTTGGCTTTA
    AAGGCGCAGT TCAACACAGA GGATGTGGAC TCCCTGTTGG CCCAGCTGAT GGATGAGCTC
    ATCTCAGACT GCAGCCAGCC CCTGGGCAAT GTCCAAGAGA TACCCTCTGC CACCCACCTG
    GACCGGCACC TGTACAGGCT GCGCACCCGT CACCTAGGCC AGATTACTGA GGCGGCCCTG
    GCTCTGAAAC TCGGGCATGG CAAGCTCCCT GCCGCCCTGG AGCAGGCTGA AGACTGGCTC
    CTGCGTCTCC GTGCCCTGGC TGATGAGCCC CAGAACAGCC TGCCCGACAT CATCATCTGG
    ATGCTGCAGG GAAACAAGCG AGTGGCGTAC CAGCGGGTGC CTGCGCACGA GGTCCTCTTC
    TCCCGGCGGG GCGCCAGCTA CTGTGGCAAG AACTGTGGGA AGCTGCAGAC GATATTTCTG
    AAATATCCCA TGGAGGGGGT GCCCGGAGCC CGGATGCCAG TACAAATACG GGTGAAACTC
    TGGTTTGGGC TTTCTGTGGA TGAGAAGGAG TTCAACCAGT TTGCTGAGGG AAAGCTGTCC
    GTCTTTGCTG AAACTTATGA GAACCAGACC AAACTGGCCC TGGTTGGGAA CTGGGGCACA
    ACAGGCCTCA CCTACCCCAA GTTTTCTGAT GTCATGGGCA AGATCAAGCT ACCCAAGGAT
    AGCTTCCGCC CCTCAGCTGG CTGGACCTGG GCCGGAGACT GGTTCGTGTG TCCAGAGAAG
    ACCCTACTCC ATGACATGGA CGCGGGCCAC CTGAGTTTTG TGGAGGAGGT GTTTGAGAAC
    CAGACCCGGC TCCCTGGAGG CCAGTGGATC TACATGAGTG ACAACTACAC AGATGTGAAT
    GGGGATAAGG TGCTTCCTAA GGATGACATT GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT
    GAAGAGTGGT CCACGGACCT CAATCGGGCC GTCGATGAGC AAGGCTGGGA GTACAGTATC
    ACCATCCCCC CGGATCGGAA GCCAAAGCAC TGGGTTCCTG CTGAGAAGAT GTATTACACG
    CACCGTCGGC GGCGCTGGGT CCGCCTGCGC AGAAGGGACC TCAGCCAGAT GGAGGCATTG
    AAGAGGCACA GACAGGCAGA GGCAGAGGGT GAGGGCTGGG AGTACGCCTC GCTCTTGGGC
    TGGAAGTTCC ACCTGGAGTA CCGCAAGACT GATGCTTTCC GCCGCCGCCG CTGGCGCCGC
    CGCATGGAAC CGCTGGAGAA GACGGGGCCT GCAGCCGTGT TTGCCCTTGA GGGGGCTCTG
    GGTGGTGTGG TAGATGACAG GAGTGAAGAT TCCGTGTCCG TGTCCACCTT GAGCTTTGGA
    GTGAACAGAC CCACGATTTC CTGCATCTTT GATTATGGGA ACCGCTACCA CCTACGCTGC
    TACATGTACC AGGCTCGGGA CCTGCCCTCG ATGGACAAGG ACTCTTTTTC TGATCCCTAC
    GCCATCGTCT CCTTCCTGCA CCAGAGCCAG AAGACAGTGG TGGCGAAGAA CACTCTGAAC
    CCCACCTGGG ACCAGACGCT TATCTTCTAT GAGATTGAGA TCTTTGGTGA GCCAGCCAGT
    ATCGCTGAGC AGCCGCCCAG CATTGTGGTG GAGCTGTATG ACCACGACAC CTACGGTGCA
    GATGAGTTTA TGGGACGCTG CATTTGTCAG CCCAGCCTGG AGCGGACACC CCGGCTGGCC
    TGGTTCCCAC TGATTAGGGG CAGCCAGTCG GCGGGGGAAC TGCTGGCCTC TTTTGAGCTC
    ATCCAGAGAG AAAAGCCGGC CATTCACCAT ATTCCTGGTT TTGAGGTACA GGATATGTCA
    GGAATCCTGG AAGAGTCAGA GGACATAGAC CTGCCCTACC CGCCACCTCA GAGGGAGGCC
    AACATCTTCA TGGTCCCCCA GAACATCAAG CCTGTTCTCC AGCGTACTGC CATAGAGATC
    CTGGCGTGGG GCCTGCGGAA CATGAAGAGC TACCAGCTAG CCAGCATCTC CTCCCCTAGC
    CTTGTGGTAG AGTGCGGGGG CCAGACAGTG CAGTCCTGTG TCATCAAGAA CCTCCGGAAG
    AACCCCAACT TCGACATCTG TACCCTTTTC ATGGAAGTGA TGCTGCCCAA GGAGGAGGTC
    TACTGTCCCT CCATCGTGAT CAAGGTCATT GATAACCGCC AGTTTGGCCG CCGGCCTGTG
    GTGGGCCAGT GTACCATCCG TTCCCTGGAG AGCTTCATGT GTGACCTGTA CTCCGAGGAG
    AATCCGTCCC CACAGGGCGG CCCAGATGAT GTGAGTTTAC TCAGTCCTGG GGAAGACGTG
    CTCATTGACA TCGATGACAA GGAGCCCCTC ATCCCCATGC AGCTTGCAGA TGGTCTGTCG
    GGCTCTGCCC CCACTAACAT GGCATCTTCT CCATCCAGTC CTCATGAGGA AGAGTTCATC
    GACTGGTGGA GCAAATTCTT TGCCTCCACA GGGGAGAGGG AAAAGTGCGG CTCATACTTG
    GAGAAGGGCT TTGACACTCT AAAGGTCTAT GACACACAAC TGGAGAACGT GGAGGCCTTT
    GAGGGCCTGT CTGACTTTTG TAACACCTTC AAGCTCTACC GGGGCAAGAC TCAGGAGGAG
    ACAGAGGACC CATCTGTTAT TGGGGAGTTT AAGGGCCTCT TCAAAATCTA TCCCCTCCCA
    GAAGACCCGG CTGTTCCCAT GCCCCCGAGA CAATTCCACC AGCTGGCTGC CCAAGGGCCT
    CAGGAGTGCC TGGTCCGCAT CTATATCATC CGAGCATTTG GCCTTCAGCC CAAGGACCCC
    AATGGGAAGT GTGATCCTTA CATCAAGATC TCCATAGGGA AGAAATCAGT GAGTGACCAG
    GATAACTACA TTCCCTGTAC TCTGGAGCCT GTGTTTGGAA AGATGTTCGA GCTGACCTGC
    ACTCTGCCTC TGGAGAAGGA CCTGAAGATC ACACTCTATG ACTATGACCT CCTCTCCAAA
    GATGAAAAGA TTGGGGAGAC AGTCATTGAC CTGGAGGATA GGCTGCTGTC CAAGTTTGGG
    GCTCGCTGCG GACTCCCACA GACGTACTGT GTCTCTGGAC CCAACCAGTG GAGGGACCAG
    CTCCGCCCCT CCCAGCTTCT CCACCTCTTC TGCCAGCAGC GCAGACTCAA GGCTCCTGTG
    TACCTCACGG ACCACGTGGT GTTCCAGGAT AAAGAATACA GCATTGAAGA AATAGAGGCT
    GGCCGAGTCC CGAACCCACA CCTGGGCCCA GTGGAAGAGC GTCTGGCCTT GCATGTCCTC
    CAGCAGCAGG GCCTGGTCCC TGAGCATGTG GAGTCCCGGC CTCTCTACAG CCCCCTGCAG
    CCAGACATTG AACAGGGGAA GCTGCAGATG TGGATCGACC TATTTCCCAA GGCCCTGGGG
    CGTCCCGGAC CTCCCTTCAA CATCACCCCG CGGAGAGCCA GAAGGTTTTT CCTGCGTTGT
    ATCATCTGGA ACACAAAGGA CGTGATCCTG GACGACCTCA GCATCACAGG GGAGAAGATG
    AGTGATATTT ATGTGAAGGG TTGGATGGTT GGCTTTGAAG AGCACAAGCA AAAGACAGAT
    GTGCATTACC GGTCCCTGGG AGGTGAAGGC AACTTTAACT GGAGGTTCAT TTTCCCCTTT
    GACTACCTGC CAGCCGAGCA AGTCTGCACC GTCTCTAAGA AGGATGCTTT CTGGAGGCTG
    GACAAGACTG AGAGCAAAAT CCCAGCACGA GTGGTATTTC AGATCTGGGA CAATGACAAG
    TTCTCCTTTG ACGACTTTCT GGGCTCCTTG CAGTTGGATC TCAACCACAT GCCCAAGCCT
    GCCAAGACAG CTGAGAAGTG CTCCTTGGAC CAGCTGGATG ACACTTTCCA CCCGGAGTGG
    TTTGTGTCCC TTTTTGAACA GAAAACAGTT AAGGGCTGGT GGCCCTGCGT AGCAGAAGAG
    GGCGAGAAGA AGATCCTGGC AGGCAAGCTG GAAATGACCT TGGAGATCGT AGCAGAGAGT
    GAGCATGAGG AGCGGCCTGC TGGCCAGGGC CGGGATGAAC CCAACATGAA CCCCAAGCTC
    GAGGACCCAA GGCGCCCTGA CACCTCCTTC CTCTGGTTCA CCTCTCCATA CAAGACTATG
    AAGTTCATCC TGTGGCGGCG GTTCCGATGT GCCATTATCC TCTTCATCAT TCTCTTCATC
    CTGCTACTAT TCTTAGGCAT CTTCATCTAT GCTTTCCCGA ACTACGCTGC CATGAAGCTG
    GTGAAGCCCT TCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003432274.1
    CDS23..6340
    Translation

    Target ORF information:

    RefSeq Version XM_003432226.4
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris dysferlin (DYSF), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003432226.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG ACACAGGAGG AGAGGAGGAC
    ACTGAGGACC AGGGGCTCAC TGGAGACGAG GGAGAGCCAT TACTGGATCA GAGTGGCGTC
    CCAGGCCCTG GGGCTCCCAC CACCCCGAGG AAGCCACCTT CCCATCTGCC CCCCTACCAC
    CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC GCAGCCAGAA AGCTGCTGTC CGACAAACCG
    CAGGACTTCC AGATCAGAGT GCAAGTGATC GAGGGCCGCC AGCTGCCAGG GGTGAATATC
    AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG CAGACCAAGA GGACACGGAT CCACAAGGGA
    AACAGCCCAC TATTTAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TTCGGAGCTG
    TTTGATGAGC CCATCTTTAT CACGGTCGTA GACTCCCGTT CTCTCAGGAC AGATGCTCTC
    ATTGGGGAAT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA TGCTTATCTG
    AGGAAGTGGC TGCTACTCTC CAACCCTGAT GACTTCTCTG TTGGGGCCAG AGGTTACCTG
    AAAGCAAGCC TTTATGTGCT GGGGCCCGGA GATGAAGCGC CTCTGGAGAG AAAGGACCCC
    TCTGAAGACA AGGAGGACAT TGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTGCGA
    GGAGCCCACT TCTGCCTGAA GATCTTCAGA GCGGAGGACT TACCACAAAT GGATGATGCC
    GTGATGGACA ATGTGAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA TTTGGTAGAT
    CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA
    GCCAACCCTC AGTGGAACCA GAGCATCACT CTGCCTGCCA TGTTTCCCTC CATGTGTGAA
    AAAATGAAGA TTCGCGTCAT GGACTGGGAC CGCCTTACCC ACAATGACAT CGTGGCCACC
    ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGAAGA GGAGCCTGCA
    GGTGTGGTCA AGCCTTCTCC AGCCACGGAC CTGGATGACC ATCTGGGCTT CCTTCCCACC
    TTCGGGCCCT GCTTCGTCAA TCTCTATGGC AGCCCCAGGG AATTCACTGG CTTCCCGGAC
    CCCTACGCGG AACTCAACAT GGGCAAGGGG GAAGGCGTGG CCTACCGAGG TCGGCTTCTG
    CTCTCCCTGG AGACCAAGCT GGTGGAGCAC AGCGGCGAGC AAAGGGTGGA GGACCTCCCT
    GCTGATGACA TCCTTCGGGT GGAGAAATAC CTCCGGAGGC GCAAGTACTC TCTCTTTGCC
    GCCTTCTACT CGGCCACCAT GCTCCAGGAT GTCGACGATG CCATCCAGTT TGAGGTCAGC
    ATTGGGAACT ATGGCAACAA GTTCGACACG ACCTGTCTGC CACTGGCCTC CACCACCCAG
    TACAGCCGGG CAGTCTTTGA TGGGTGCCAC TACTACTACT TGCCCTGGGG CAATGTGAAG
    CCTGTGGTGG TGCTGTCATC CTACTGGGAA GACATCAGCC ACAGAGTCGA GGCTCAGAAC
    CAGCTGCTCA GGATCGCTGA CCATCTGGAA GCTGGCCTGG AGCAAGTCCA CTTGGCTTTA
    AAGGCGCAGT TCAACACAGA GGATGTGGAC TCCCTGTTGG CCCAGCTGAT GGATGAGCTC
    ATCTCAGACT GCAGCCAGCC CCTGGGCAAT GTCCAAGAGA TACCCTCTGC CACCCACCTG
    GACCGGCACC TGTACAGGCT GCGCACCCGT CACCTAGGCC AGATTACTGA GGCGGCCCTG
    GCTCTGAAAC TCGGGCATGG CAAGCTCCCT GCCGCCCTGG AGCAGGCTGA AGACTGGCTC
    CTGCGTCTCC GTGCCCTGGC TGATGAGCCC CAGAACAGCC TGCCCGACAT CATCATCTGG
    ATGCTGCAGG GAAACAAGCG AGTGGCGTAC CAGCGGGTGC CTGCGCACGA GGTCCTCTTC
    TCCCGGCGGG GCGCCAGCTA CTGTGGCAAG AACTGTGGGA AGCTGCAGAC GATATTTCTG
    AAATATCCCA TGGAGGGGGT GCCCGGAGCC CGGATGCCAG TACAAATACG GGTGAAACTC
    TGGTTTGGGC TTTCTGTGGA TGAGAAGGAG TTCAACCAGT TTGCTGAGGG AAAGCTGTCC
    GTCTTTGCTG AAACTTATGA GAACCAGACC AAACTGGCCC TGGTTGGGAA CTGGGGCACA
    ACAGGCCTCA CCTACCCCAA GTTTTCTGAT GTCATGGGCA AGATCAAGCT ACCCAAGGAT
    AGCTTCCGCC CCTCAGCTGG CTGGACCTGG GCCGGAGACT GGTTCGTGTG TCCAGAGAAG
    ACCCTACTCC ATGACATGGA CGCGGGCCAC CTGAGTTTTG TGGAGGAGGT GTTTGAGAAC
    CAGACCCGGC TCCCTGGAGG CCAGTGGATC TACATGAGTG ACAACTACAC AGATGTGAAT
    GGGGATAAGG TGCTTCCTAA GGATGACATT GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT
    GAAGAGTGGT CCACGGACCT CAATCGGGCC GTCGATGAGC AAGGCTGGGA GTACAGTATC
    ACCATCCCCC CGGATCGGAA GCCAAAGCAC TGGGTTCCTG CTGAGAAGAT GTATTACACG
    CACCGTCGGC GGCGCTGGGT CCGCCTGCGC AGAAGGGACC TCAGCCAGAT GGAGGCATTG
    AAGAGGCACA GACAGGCAGA GGCAGAGGGT GAGGGCTGGG AGTACGCCTC GCTCTTGGGC
    TGGAAGTTCC ACCTGGAGTA CCGCAAGACT GATGCTTTCC GCCGCCGCCG CTGGCGCCGC
    CGCATGGAAC CGCTGGAGAA GACGGGGCCT GCAGCCGTGT TTGCCCTTGA GGGGGCTCTG
    GGTGGTGTGG TAGATGACAG GAGTGAAGAT TCCGTGTCCG TGTCCACCTT GAGCTTTGGA
    GTGAACAGAC CCACGATTTC CTGCATCTTT GATTATGGGA ACCGCTACCA CCTACGCTGC
    TACATGTACC AGGCTCGGGA CCTGCCCTCG ATGGACAAGG ACTCTTTTTC TGATCCCTAC
    GCCATCGTCT CCTTCCTGCA CCAGAGCCAG AAGACAGTGG TGGCGAAGAA CACTCTGAAC
    CCCACCTGGG ACCAGACGCT TATCTTCTAT GAGATTGAGA TCTTTGGTGA GCCAGCCAGT
    ATCGCTGAGC AGCCGCCCAG CATTGTGGTG GAGCTGTATG ACCACGACAC CTACGGTGCA
    GATGAGTTTA TGGGACGCTG CATTTGTCAG CCCAGCCTGG AGCGGACACC CCGGCTGGCC
    TGGTTCCCAC TGATTAGGGG CAGCCAGTCG GCGGGGGAAC TGCTGGCCTC TTTTGAGCTC
    ATCCAGAGAG AAAAGCCGGC CATTCACCAT ATTCCTGGTT TTGAGGTACA GGATATGTCA
    GGAATCCTGG AAGAGTCAGA GGACATAGAC CTGCCCTACC CGCCACCTCA GAGGGAGGCC
    AACATCTTCA TGGTCCCCCA GAACATCAAG CCTGTTCTCC AGCGTACTGC CATAGAGATC
    CTGGCGTGGG GCCTGCGGAA CATGAAGAGC TACCAGCTAG CCAGCATCTC CTCCCCTAGC
    CTTGTGGTAG AGTGCGGGGG CCAGACAGTG CAGTCCTGTG TCATCAAGAA CCTCCGGAAG
    AACCCCAACT TCGACATCTG TACCCTTTTC ATGGAAGTGA TGCTGCCCAA GGAGGAGGTC
    TACTGTCCCT CCATCGTGAT CAAGGTCATT GATAACCGCC AGTTTGGCCG CCGGCCTGTG
    GTGGGCCAGT GTACCATCCG TTCCCTGGAG AGCTTCATGT GTGACCTGTA CTCCGAGGAG
    AATCCGTCCC CACAGGGCGG CCCAGATGAT GTGAGTTTAC TCAGTCCTGG GGAAGACGTG
    CTCATTGACA TCGATGACAA GGAGCCCCTC ATCCCCATGC AGCTTGCAGA TGGTCTGTCG
    GGCTCTGCCC CCACTAACAT GGCATCTTCT CCATCCAGTC CTCATGAGGA AGAGTTCATC
    GACTGGTGGA GCAAATTCTT TGCCTCCACA GGGGAGAGGG AAAAGTGCGG CTCATACTTG
    GAGAAGGGCT TTGACACTCT AAAGGTCTAT GACACACAAC TGGAGAACGT GGAGGCCTTT
    GAGGGCCTGT CTGACTTTTG TAACACCTTC AAGCTCTACC GGGGCAAGAC TCAGGAGGAG
    ACAGAGGACC CATCTGTTAT TGGGGAGTTT AAGGGCCTCT TCAAAATCTA TCCCCTCCCA
    GAAGACCCGG CTGTTCCCAT GCCCCCGAGA CAATTCCACC AGCTGGCTGC CCAAGGGCCT
    CAGGAGTGCC TGGTCCGCAT CTATATCATC CGAGCATTTG GCCTTCAGCC CAAGGACCCC
    AATGGGAAGT GTGATCCTTA CATCAAGATC TCCATAGGGA AGAAATCAGT GAGTGACCAG
    GATAACTACA TTCCCTGTAC TCTGGAGCCT GTGTTTGGAA AGATGTTCGA GCTGACCTGC
    ACTCTGCCTC TGGAGAAGGA CCTGAAGATC ACACTCTATG ACTATGACCT CCTCTCCAAA
    GATGAAAAGA TTGGGGAGAC AGTCATTGAC CTGGAGGATA GGCTGCTGTC CAAGTTTGGG
    GCTCGCTGCG GACTCCCACA GACGTACTGT GTCTCTGGAC CCAACCAGTG GAGGGACCAG
    CTCCGCCCCT CCCAGCTTCT CCACCTCTTC TGCCAGCAGC GCAGACTCAA GGCTCCTGTG
    TACCTCACGG ACCACGTGGT GTTCCAGGAT AAAGAATACA GCATTGAAGA AATAGAGGCT
    GGCCGAGTCC CGAACCCACA CCTGGGCCCA GTGGAAGAGC GTCTGGCCTT GCATGTCCTC
    CAGCAGCAGG GCCTGGTCCC TGAGCATGTG GAGTCCCGGC CTCTCTACAG CCCCCTGCAG
    CCAGACATTG AACAGGGGAA GCTGCAGATG TGGATCGACC TATTTCCCAA GGCCCTGGGG
    CGTCCCGGAC CTCCCTTCAA CATCACCCCG CGGAGAGCCA GAAGGTTTTT CCTGCGTTGT
    ATCATCTGGA ACACAAAGGA CGTGATCCTG GACGACCTCA GCATCACAGG GGAGAAGATG
    AGTGATATTT ATGTGAAGGG TTGGATGGTT GGCTTTGAAG AGCACAAGCA AAAGACAGAT
    GTGCATTACC GGTCCCTGGG AGGTGAAGGC AACTTTAACT GGAGGTTCAT TTTCCCCTTT
    GACTACCTGC CAGCCGAGCA AGTCTGCACC GTCTCTAAGA AGGATGCTTT CTGGAGGCTG
    GACAAGACTG AGAGCAAAAT CCCAGCACGA GTGGTATTTC AGATCTGGGA CAATGACAAG
    TTCTCCTTTG ACGACTTTCT GGGCTCCTTG CAGTTGGATC TCAACCACAT GCCCAAGCCT
    GCCAAGACAG CTGAGAAGTG CTCCTTGGAC CAGCTGGATG ACACTTTCCA CCCGGAGTGG
    TTTGTGTCCC TTTTTGAACA GAAAACAGTT AAGGGCTGGT GGCCCTGCGT AGCAGAAGAG
    GGCGAGAAGA AGATCCTGGC AGGCAAGCTG GAAATGACCT TGGAGATCGT AGCAGAGAGT
    GAGCATGAGG AGCGGCCTGC TGGCCAGGGC CGGGATGAAC CCAACATGAA CCCCAAGCTC
    GAGGACCCAA GGCGCCCTGA CACCTCCTTC CTCTGGTTCA CCTCTCCATA CAAGACTATG
    AAGTTCATCC TGTGGCGGCG GTTCCGATGT GCCATTATCC TCTTCATCAT TCTCTTCATC
    CTGCTACTAT TCTTAGGCAT CTTCATCTAT GCTTTCCCGA ACTACGCTGC CATGAAGCTG
    GTGAAGCCCT TCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe19210
    Clone ID Related Accession (Same CDS sequence) XM_003432226.4 , XM_003432226.3
    Accession Version XM_003432226.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6318bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product dysferlin isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003432226.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG ACACAGGAGG AGAGGAGGAC
    ACTGAGGACC AGGGGCTCAC TGGAGACGAG GGAGAGCCAT TACTGGATCA GAGTGGCGTC
    CCAGGCCCTG GGGCTCCCAC CACCCCGAGG AAGCCACCTT CCCATCTGCC CCCCTACCAC
    CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC GCAGCCAGAA AGCTGCTGTC CGACAAACCG
    CAGGACTTCC AGATCAGAGT GCAAGTGATC GAGGGCCGCC AGCTGCCAGG GGTGAATATC
    AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG CAGACCAAGA GGACACGGAT CCACAAGGGA
    AACAGCCCAC TATTTAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TTCGGAGCTG
    TTTGATGAGC CCATCTTTAT CACGGTCGTA GACTCCCGTT CTCTCAGGAC AGATGCTCTC
    ATTGGGGAAT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA TGCTTATCTG
    AGGAAGTGGC TGCTACTCTC CAACCCTGAT GACTTCTCTG TTGGGGCCAG AGGTTACCTG
    AAAGCAAGCC TTTATGTGCT GGGGCCCGGA GATGAAGCGC CTCTGGAGAG AAAGGACCCC
    TCTGAAGACA AGGAGGACAT TGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTGCGA
    GGAGCCCACT TCTGCCTGAA GATCTTCAGA GCGGAGGACT TACCACAAAT GGATGATGCC
    GTGATGGACA ATGTGAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA TTTGGTAGAT
    CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA
    GCCAACCCTC AGTGGAACCA GAGCATCACT CTGCCTGCCA TGTTTCCCTC CATGTGTGAA
    AAAATGAAGA TTCGCGTCAT GGACTGGGAC CGCCTTACCC ACAATGACAT CGTGGCCACC
    ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGAAGA GGAGCCTGCA
    GGTGTGGTCA AGCCTTCTCC AGCCACGGAC CTGGATGACC ATCTGGGCTT CCTTCCCACC
    TTCGGGCCCT GCTTCGTCAA TCTCTATGGC AGCCCCAGGG AATTCACTGG CTTCCCGGAC
    CCCTACGCGG AACTCAACAT GGGCAAGGGG GAAGGCGTGG CCTACCGAGG TCGGCTTCTG
    CTCTCCCTGG AGACCAAGCT GGTGGAGCAC AGCGGCGAGC AAAGGGTGGA GGACCTCCCT
    GCTGATGACA TCCTTCGGGT GGAGAAATAC CTCCGGAGGC GCAAGTACTC TCTCTTTGCC
    GCCTTCTACT CGGCCACCAT GCTCCAGGAT GTCGACGATG CCATCCAGTT TGAGGTCAGC
    ATTGGGAACT ATGGCAACAA GTTCGACACG ACCTGTCTGC CACTGGCCTC CACCACCCAG
    TACAGCCGGG CAGTCTTTGA TGGGTGCCAC TACTACTACT TGCCCTGGGG CAATGTGAAG
    CCTGTGGTGG TGCTGTCATC CTACTGGGAA GACATCAGCC ACAGAGTCGA GGCTCAGAAC
    CAGCTGCTCA GGATCGCTGA CCATCTGGAA GCTGGCCTGG AGCAAGTCCA CTTGGCTTTA
    AAGGCGCAGT TCAACACAGA GGATGTGGAC TCCCTGTTGG CCCAGCTGAT GGATGAGCTC
    ATCTCAGACT GCAGCCAGCC CCTGGGCAAT GTCCAAGAGA TACCCTCTGC CACCCACCTG
    GACCGGCACC TGTACAGGCT GCGCACCCGT CACCTAGGCC AGATTACTGA GGCGGCCCTG
    GCTCTGAAAC TCGGGCATGG CAAGCTCCCT GCCGCCCTGG AGCAGGCTGA AGACTGGCTC
    CTGCGTCTCC GTGCCCTGGC TGATGAGCCC CAGAACAGCC TGCCCGACAT CATCATCTGG
    ATGCTGCAGG GAAACAAGCG AGTGGCGTAC CAGCGGGTGC CTGCGCACGA GGTCCTCTTC
    TCCCGGCGGG GCGCCAGCTA CTGTGGCAAG AACTGTGGGA AGCTGCAGAC GATATTTCTG
    AAATATCCCA TGGAGGGGGT GCCCGGAGCC CGGATGCCAG TACAAATACG GGTGAAACTC
    TGGTTTGGGC TTTCTGTGGA TGAGAAGGAG TTCAACCAGT TTGCTGAGGG AAAGCTGTCC
    GTCTTTGCTG AAACTTATGA GAACCAGACC AAACTGGCCC TGGTTGGGAA CTGGGGCACA
    ACAGGCCTCA CCTACCCCAA GTTTTCTGAT GTCATGGGCA AGATCAAGCT ACCCAAGGAT
    AGCTTCCGCC CCTCAGCTGG CTGGACCTGG GCCGGAGACT GGTTCGTGTG TCCAGAGAAG
    ACCCTACTCC ATGACATGGA CGCGGGCCAC CTGAGTTTTG TGGAGGAGGT GTTTGAGAAC
    CAGACCCGGC TCCCTGGAGG CCAGTGGATC TACATGAGTG ACAACTACAC AGATGTGAAT
    GGGGATAAGG TGCTTCCTAA GGATGACATT GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT
    GAAGAGTGGT CCACGGACCT CAATCGGGCC GTCGATGAGC AAGGCTGGGA GTACAGTATC
    ACCATCCCCC CGGATCGGAA GCCAAAGCAC TGGGTTCCTG CTGAGAAGAT GTATTACACG
    CACCGTCGGC GGCGCTGGGT CCGCCTGCGC AGAAGGGACC TCAGCCAGAT GGAGGCATTG
    AAGAGGCACA GACAGGCAGA GGCAGAGGGT GAGGGCTGGG AGTACGCCTC GCTCTTGGGC
    TGGAAGTTCC ACCTGGAGTA CCGCAAGACT GATGCTTTCC GCCGCCGCCG CTGGCGCCGC
    CGCATGGAAC CGCTGGAGAA GACGGGGCCT GCAGCCGTGT TTGCCCTTGA GGGGGCTCTG
    GGTGGTGTGG TAGATGACAG GAGTGAAGAT TCCGTGTCCG TGTCCACCTT GAGCTTTGGA
    GTGAACAGAC CCACGATTTC CTGCATCTTT GATTATGGGA ACCGCTACCA CCTACGCTGC
    TACATGTACC AGGCTCGGGA CCTGCCCTCG ATGGACAAGG ACTCTTTTTC TGATCCCTAC
    GCCATCGTCT CCTTCCTGCA CCAGAGCCAG AAGACAGTGG TGGCGAAGAA CACTCTGAAC
    CCCACCTGGG ACCAGACGCT TATCTTCTAT GAGATTGAGA TCTTTGGTGA GCCAGCCAGT
    ATCGCTGAGC AGCCGCCCAG CATTGTGGTG GAGCTGTATG ACCACGACAC CTACGGTGCA
    GATGAGTTTA TGGGACGCTG CATTTGTCAG CCCAGCCTGG AGCGGACACC CCGGCTGGCC
    TGGTTCCCAC TGATTAGGGG CAGCCAGTCG GCGGGGGAAC TGCTGGCCTC TTTTGAGCTC
    ATCCAGAGAG AAAAGCCGGC CATTCACCAT ATTCCTGGTT TTGAGGTACA GGATATGTCA
    GGAATCCTGG AAGAGTCAGA GGACATAGAC CTGCCCTACC CGCCACCTCA GAGGGAGGCC
    AACATCTTCA TGGTCCCCCA GAACATCAAG CCTGTTCTCC AGCGTACTGC CATAGAGATC
    CTGGCGTGGG GCCTGCGGAA CATGAAGAGC TACCAGCTAG CCAGCATCTC CTCCCCTAGC
    CTTGTGGTAG AGTGCGGGGG CCAGACAGTG CAGTCCTGTG TCATCAAGAA CCTCCGGAAG
    AACCCCAACT TCGACATCTG TACCCTTTTC ATGGAAGTGA TGCTGCCCAA GGAGGAGGTC
    TACTGTCCCT CCATCGTGAT CAAGGTCATT GATAACCGCC AGTTTGGCCG CCGGCCTGTG
    GTGGGCCAGT GTACCATCCG TTCCCTGGAG AGCTTCATGT GTGACCTGTA CTCCGAGGAG
    AATCCGTCCC CACAGGGCGG CCCAGATGAT GTGAGTTTAC TCAGTCCTGG GGAAGACGTG
    CTCATTGACA TCGATGACAA GGAGCCCCTC ATCCCCATGC AGCTTGCAGA TGGTCTGTCG
    GGCTCTGCCC CCACTAACAT GGCATCTTCT CCATCCAGTC CTCATGAGGA AGAGTTCATC
    GACTGGTGGA GCAAATTCTT TGCCTCCACA GGGGAGAGGG AAAAGTGCGG CTCATACTTG
    GAGAAGGGCT TTGACACTCT AAAGGTCTAT GACACACAAC TGGAGAACGT GGAGGCCTTT
    GAGGGCCTGT CTGACTTTTG TAACACCTTC AAGCTCTACC GGGGCAAGAC TCAGGAGGAG
    ACAGAGGACC CATCTGTTAT TGGGGAGTTT AAGGGCCTCT TCAAAATCTA TCCCCTCCCA
    GAAGACCCGG CTGTTCCCAT GCCCCCGAGA CAATTCCACC AGCTGGCTGC CCAAGGGCCT
    CAGGAGTGCC TGGTCCGCAT CTATATCATC CGAGCATTTG GCCTTCAGCC CAAGGACCCC
    AATGGGAAGT GTGATCCTTA CATCAAGATC TCCATAGGGA AGAAATCAGT GAGTGACCAG
    GATAACTACA TTCCCTGTAC TCTGGAGCCT GTGTTTGGAA AGATGTTCGA GCTGACCTGC
    ACTCTGCCTC TGGAGAAGGA CCTGAAGATC ACACTCTATG ACTATGACCT CCTCTCCAAA
    GATGAAAAGA TTGGGGAGAC AGTCATTGAC CTGGAGGATA GGCTGCTGTC CAAGTTTGGG
    GCTCGCTGCG GACTCCCACA GACGTACTGT GTCTCTGGAC CCAACCAGTG GAGGGACCAG
    CTCCGCCCCT CCCAGCTTCT CCACCTCTTC TGCCAGCAGC GCAGACTCAA GGCTCCTGTG
    TACCTCACGG ACCACGTGGT GTTCCAGGAT AAAGAATACA GCATTGAAGA AATAGAGGCT
    GGCCGAGTCC CGAACCCACA CCTGGGCCCA GTGGAAGAGC GTCTGGCCTT GCATGTCCTC
    CAGCAGCAGG GCCTGGTCCC TGAGCATGTG GAGTCCCGGC CTCTCTACAG CCCCCTGCAG
    CCAGACATTG AACAGGGGAA GCTGCAGATG TGGATCGACC TATTTCCCAA GGCCCTGGGG
    CGTCCCGGAC CTCCCTTCAA CATCACCCCG CGGAGAGCCA GAAGGTTTTT CCTGCGTTGT
    ATCATCTGGA ACACAAAGGA CGTGATCCTG GACGACCTCA GCATCACAGG GGAGAAGATG
    AGTGATATTT ATGTGAAGGG TTGGATGGTT GGCTTTGAAG AGCACAAGCA AAAGACAGAT
    GTGCATTACC GGTCCCTGGG AGGTGAAGGC AACTTTAACT GGAGGTTCAT TTTCCCCTTT
    GACTACCTGC CAGCCGAGCA AGTCTGCACC GTCTCTAAGA AGGATGCTTT CTGGAGGCTG
    GACAAGACTG AGAGCAAAAT CCCAGCACGA GTGGTATTTC AGATCTGGGA CAATGACAAG
    TTCTCCTTTG ACGACTTTCT GGGCTCCTTG CAGTTGGATC TCAACCACAT GCCCAAGCCT
    GCCAAGACAG CTGAGAAGTG CTCCTTGGAC CAGCTGGATG ACACTTTCCA CCCGGAGTGG
    TTTGTGTCCC TTTTTGAACA GAAAACAGTT AAGGGCTGGT GGCCCTGCGT AGCAGAAGAG
    GGCGAGAAGA AGATCCTGGC AGGCAAGCTG GAAATGACCT TGGAGATCGT AGCAGAGAGT
    GAGCATGAGG AGCGGCCTGC TGGCCAGGGC CGGGATGAAC CCAACATGAA CCCCAAGCTC
    GAGGACCCAA GGCGCCCTGA CACCTCCTTC CTCTGGTTCA CCTCTCCATA CAAGACTATG
    AAGTTCATCC TGTGGCGGCG GTTCCGATGT GCCATTATCC TCTTCATCAT TCTCTTCATC
    CTGCTACTAT TCTTAGGCAT CTTCATCTAT GCTTTCCCGA ACTACGCTGC CATGAAGCTG
    GTGAAGCCCT TCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003432274.1
    CDS51..6368
    Translation

    Target ORF information:

    RefSeq Version XM_003432226.3
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris dysferlin (DYSF), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003432226.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG ACACAGGAGG AGAGGAGGAC
    ACTGAGGACC AGGGGCTCAC TGGAGACGAG GGAGAGCCAT TACTGGATCA GAGTGGCGTC
    CCAGGCCCTG GGGCTCCCAC CACCCCGAGG AAGCCACCTT CCCATCTGCC CCCCTACCAC
    CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC GCAGCCAGAA AGCTGCTGTC CGACAAACCG
    CAGGACTTCC AGATCAGAGT GCAAGTGATC GAGGGCCGCC AGCTGCCAGG GGTGAATATC
    AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG CAGACCAAGA GGACACGGAT CCACAAGGGA
    AACAGCCCAC TATTTAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TTCGGAGCTG
    TTTGATGAGC CCATCTTTAT CACGGTCGTA GACTCCCGTT CTCTCAGGAC AGATGCTCTC
    ATTGGGGAAT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA TGCTTATCTG
    AGGAAGTGGC TGCTACTCTC CAACCCTGAT GACTTCTCTG TTGGGGCCAG AGGTTACCTG
    AAAGCAAGCC TTTATGTGCT GGGGCCCGGA GATGAAGCGC CTCTGGAGAG AAAGGACCCC
    TCTGAAGACA AGGAGGACAT TGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTGCGA
    GGAGCCCACT TCTGCCTGAA GATCTTCAGA GCGGAGGACT TACCACAAAT GGATGATGCC
    GTGATGGACA ATGTGAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA TTTGGTAGAT
    CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA
    GCCAACCCTC AGTGGAACCA GAGCATCACT CTGCCTGCCA TGTTTCCCTC CATGTGTGAA
    AAAATGAAGA TTCGCGTCAT GGACTGGGAC CGCCTTACCC ACAATGACAT CGTGGCCACC
    ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGAAGA GGAGCCTGCA
    GGTGTGGTCA AGCCTTCTCC AGCCACGGAC CTGGATGACC ATCTGGGCTT CCTTCCCACC
    TTCGGGCCCT GCTTCGTCAA TCTCTATGGC AGCCCCAGGG AATTCACTGG CTTCCCGGAC
    CCCTACGCGG AACTCAACAT GGGCAAGGGG GAAGGCGTGG CCTACCGAGG TCGGCTTCTG
    CTCTCCCTGG AGACCAAGCT GGTGGAGCAC AGCGGCGAGC AAAGGGTGGA GGACCTCCCT
    GCTGATGACA TCCTTCGGGT GGAGAAATAC CTCCGGAGGC GCAAGTACTC TCTCTTTGCC
    GCCTTCTACT CGGCCACCAT GCTCCAGGAT GTCGACGATG CCATCCAGTT TGAGGTCAGC
    ATTGGGAACT ATGGCAACAA GTTCGACACG ACCTGTCTGC CACTGGCCTC CACCACCCAG
    TACAGCCGGG CAGTCTTTGA TGGGTGCCAC TACTACTACT TGCCCTGGGG CAATGTGAAG
    CCTGTGGTGG TGCTGTCATC CTACTGGGAA GACATCAGCC ACAGAGTCGA GGCTCAGAAC
    CAGCTGCTCA GGATCGCTGA CCATCTGGAA GCTGGCCTGG AGCAAGTCCA CTTGGCTTTA
    AAGGCGCAGT TCAACACAGA GGATGTGGAC TCCCTGTTGG CCCAGCTGAT GGATGAGCTC
    ATCTCAGACT GCAGCCAGCC CCTGGGCAAT GTCCAAGAGA TACCCTCTGC CACCCACCTG
    GACCGGCACC TGTACAGGCT GCGCACCCGT CACCTAGGCC AGATTACTGA GGCGGCCCTG
    GCTCTGAAAC TCGGGCATGG CAAGCTCCCT GCCGCCCTGG AGCAGGCTGA AGACTGGCTC
    CTGCGTCTCC GTGCCCTGGC TGATGAGCCC CAGAACAGCC TGCCCGACAT CATCATCTGG
    ATGCTGCAGG GAAACAAGCG AGTGGCGTAC CAGCGGGTGC CTGCGCACGA GGTCCTCTTC
    TCCCGGCGGG GCGCCAGCTA CTGTGGCAAG AACTGTGGGA AGCTGCAGAC GATATTTCTG
    AAATATCCCA TGGAGGGGGT GCCCGGAGCC CGGATGCCAG TACAAATACG GGTGAAACTC
    TGGTTTGGGC TTTCTGTGGA TGAGAAGGAG TTCAACCAGT TTGCTGAGGG AAAGCTGTCC
    GTCTTTGCTG AAACTTATGA GAACCAGACC AAACTGGCCC TGGTTGGGAA CTGGGGCACA
    ACAGGCCTCA CCTACCCCAA GTTTTCTGAT GTCATGGGCA AGATCAAGCT ACCCAAGGAT
    AGCTTCCGCC CCTCAGCTGG CTGGACCTGG GCCGGAGACT GGTTCGTGTG TCCAGAGAAG
    ACCCTACTCC ATGACATGGA CGCGGGCCAC CTGAGTTTTG TGGAGGAGGT GTTTGAGAAC
    CAGACCCGGC TCCCTGGAGG CCAGTGGATC TACATGAGTG ACAACTACAC AGATGTGAAT
    GGGGATAAGG TGCTTCCTAA GGATGACATT GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT
    GAAGAGTGGT CCACGGACCT CAATCGGGCC GTCGATGAGC AAGGCTGGGA GTACAGTATC
    ACCATCCCCC CGGATCGGAA GCCAAAGCAC TGGGTTCCTG CTGAGAAGAT GTATTACACG
    CACCGTCGGC GGCGCTGGGT CCGCCTGCGC AGAAGGGACC TCAGCCAGAT GGAGGCATTG
    AAGAGGCACA GACAGGCAGA GGCAGAGGGT GAGGGCTGGG AGTACGCCTC GCTCTTGGGC
    TGGAAGTTCC ACCTGGAGTA CCGCAAGACT GATGCTTTCC GCCGCCGCCG CTGGCGCCGC
    CGCATGGAAC CGCTGGAGAA GACGGGGCCT GCAGCCGTGT TTGCCCTTGA GGGGGCTCTG
    GGTGGTGTGG TAGATGACAG GAGTGAAGAT TCCGTGTCCG TGTCCACCTT GAGCTTTGGA
    GTGAACAGAC CCACGATTTC CTGCATCTTT GATTATGGGA ACCGCTACCA CCTACGCTGC
    TACATGTACC AGGCTCGGGA CCTGCCCTCG ATGGACAAGG ACTCTTTTTC TGATCCCTAC
    GCCATCGTCT CCTTCCTGCA CCAGAGCCAG AAGACAGTGG TGGCGAAGAA CACTCTGAAC
    CCCACCTGGG ACCAGACGCT TATCTTCTAT GAGATTGAGA TCTTTGGTGA GCCAGCCAGT
    ATCGCTGAGC AGCCGCCCAG CATTGTGGTG GAGCTGTATG ACCACGACAC CTACGGTGCA
    GATGAGTTTA TGGGACGCTG CATTTGTCAG CCCAGCCTGG AGCGGACACC CCGGCTGGCC
    TGGTTCCCAC TGATTAGGGG CAGCCAGTCG GCGGGGGAAC TGCTGGCCTC TTTTGAGCTC
    ATCCAGAGAG AAAAGCCGGC CATTCACCAT ATTCCTGGTT TTGAGGTACA GGATATGTCA
    GGAATCCTGG AAGAGTCAGA GGACATAGAC CTGCCCTACC CGCCACCTCA GAGGGAGGCC
    AACATCTTCA TGGTCCCCCA GAACATCAAG CCTGTTCTCC AGCGTACTGC CATAGAGATC
    CTGGCGTGGG GCCTGCGGAA CATGAAGAGC TACCAGCTAG CCAGCATCTC CTCCCCTAGC
    CTTGTGGTAG AGTGCGGGGG CCAGACAGTG CAGTCCTGTG TCATCAAGAA CCTCCGGAAG
    AACCCCAACT TCGACATCTG TACCCTTTTC ATGGAAGTGA TGCTGCCCAA GGAGGAGGTC
    TACTGTCCCT CCATCGTGAT CAAGGTCATT GATAACCGCC AGTTTGGCCG CCGGCCTGTG
    GTGGGCCAGT GTACCATCCG TTCCCTGGAG AGCTTCATGT GTGACCTGTA CTCCGAGGAG
    AATCCGTCCC CACAGGGCGG CCCAGATGAT GTGAGTTTAC TCAGTCCTGG GGAAGACGTG
    CTCATTGACA TCGATGACAA GGAGCCCCTC ATCCCCATGC AGCTTGCAGA TGGTCTGTCG
    GGCTCTGCCC CCACTAACAT GGCATCTTCT CCATCCAGTC CTCATGAGGA AGAGTTCATC
    GACTGGTGGA GCAAATTCTT TGCCTCCACA GGGGAGAGGG AAAAGTGCGG CTCATACTTG
    GAGAAGGGCT TTGACACTCT AAAGGTCTAT GACACACAAC TGGAGAACGT GGAGGCCTTT
    GAGGGCCTGT CTGACTTTTG TAACACCTTC AAGCTCTACC GGGGCAAGAC TCAGGAGGAG
    ACAGAGGACC CATCTGTTAT TGGGGAGTTT AAGGGCCTCT TCAAAATCTA TCCCCTCCCA
    GAAGACCCGG CTGTTCCCAT GCCCCCGAGA CAATTCCACC AGCTGGCTGC CCAAGGGCCT
    CAGGAGTGCC TGGTCCGCAT CTATATCATC CGAGCATTTG GCCTTCAGCC CAAGGACCCC
    AATGGGAAGT GTGATCCTTA CATCAAGATC TCCATAGGGA AGAAATCAGT GAGTGACCAG
    GATAACTACA TTCCCTGTAC TCTGGAGCCT GTGTTTGGAA AGATGTTCGA GCTGACCTGC
    ACTCTGCCTC TGGAGAAGGA CCTGAAGATC ACACTCTATG ACTATGACCT CCTCTCCAAA
    GATGAAAAGA TTGGGGAGAC AGTCATTGAC CTGGAGGATA GGCTGCTGTC CAAGTTTGGG
    GCTCGCTGCG GACTCCCACA GACGTACTGT GTCTCTGGAC CCAACCAGTG GAGGGACCAG
    CTCCGCCCCT CCCAGCTTCT CCACCTCTTC TGCCAGCAGC GCAGACTCAA GGCTCCTGTG
    TACCTCACGG ACCACGTGGT GTTCCAGGAT AAAGAATACA GCATTGAAGA AATAGAGGCT
    GGCCGAGTCC CGAACCCACA CCTGGGCCCA GTGGAAGAGC GTCTGGCCTT GCATGTCCTC
    CAGCAGCAGG GCCTGGTCCC TGAGCATGTG GAGTCCCGGC CTCTCTACAG CCCCCTGCAG
    CCAGACATTG AACAGGGGAA GCTGCAGATG TGGATCGACC TATTTCCCAA GGCCCTGGGG
    CGTCCCGGAC CTCCCTTCAA CATCACCCCG CGGAGAGCCA GAAGGTTTTT CCTGCGTTGT
    ATCATCTGGA ACACAAAGGA CGTGATCCTG GACGACCTCA GCATCACAGG GGAGAAGATG
    AGTGATATTT ATGTGAAGGG TTGGATGGTT GGCTTTGAAG AGCACAAGCA AAAGACAGAT
    GTGCATTACC GGTCCCTGGG AGGTGAAGGC AACTTTAACT GGAGGTTCAT TTTCCCCTTT
    GACTACCTGC CAGCCGAGCA AGTCTGCACC GTCTCTAAGA AGGATGCTTT CTGGAGGCTG
    GACAAGACTG AGAGCAAAAT CCCAGCACGA GTGGTATTTC AGATCTGGGA CAATGACAAG
    TTCTCCTTTG ACGACTTTCT GGGCTCCTTG CAGTTGGATC TCAACCACAT GCCCAAGCCT
    GCCAAGACAG CTGAGAAGTG CTCCTTGGAC CAGCTGGATG ACACTTTCCA CCCGGAGTGG
    TTTGTGTCCC TTTTTGAACA GAAAACAGTT AAGGGCTGGT GGCCCTGCGT AGCAGAAGAG
    GGCGAGAAGA AGATCCTGGC AGGCAAGCTG GAAATGACCT TGGAGATCGT AGCAGAGAGT
    GAGCATGAGG AGCGGCCTGC TGGCCAGGGC CGGGATGAAC CCAACATGAA CCCCAAGCTC
    GAGGACCCAA GGCGCCCTGA CACCTCCTTC CTCTGGTTCA CCTCTCCATA CAAGACTATG
    AAGTTCATCC TGTGGCGGCG GTTCCGATGT GCCATTATCC TCTTCATCAT TCTCTTCATC
    CTGCTACTAT TCTTAGGCAT CTTCATCTAT GCTTTCCCGA ACTACGCTGC CATGAAGCTG
    GTGAAGCCCT TCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe19211
    Clone ID Related Accession (Same CDS sequence) XM_003432222.3 , XM_003432222.4
    Accession Version XM_003432222.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6315bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product dysferlin isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003432222.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC
    CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC
    TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA
    CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGACA CAGGAGGAGA GGAGGACACT
    GAGGACCAGG GGCTCACTGG AGACGAGGGA GAGCCATTAC TGGATCAGAG TGGCGTCCCA
    GGCCCTGGGG CTCCCACCAC CCCGAGGAAG CCACCTTCCC ATCTGCCCCC CTACCACCCT
    GGGAGCAAGA GGAAAAGAAG TGCTCCCGCA GCCAGAAAGC TGCTGTCCGA CAAACCGCAG
    GACTTCCAGA TCAGAGTGCA AGTGATCGAG GGCCGCCAGC TGCCAGGGGT GAATATCAAG
    CCTGTGATCA AGGTCACTGC TGCTGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC
    AGCCCACTAT TTAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTTC GGAGCTGTTT
    GATGAGCCCA TCTTTATCAC GGTCGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT
    GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACATGC TTATCTGAGG
    AAGTGGCTGC TACTCTCCAA CCCTGATGAC TTCTCTGTTG GGGCCAGAGG TTACCTGAAA
    GCAAGCCTTT ATGTGCTGGG GCCCGGAGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT
    GAAGACAAGG AGGACATTGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTGCGAGGA
    GCCCACTTCT GCCTGAAGAT CTTCAGAGCG GAGGACTTAC CACAAATGGA TGATGCCGTG
    ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAATTT GGTAGATCCC
    TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC
    AACCCTCAGT GGAACCAGAG CATCACTCTG CCTGCCATGT TTCCCTCCAT GTGTGAAAAA
    ATGAAGATTC GCGTCATGGA CTGGGACCGC CTTACCCACA ATGACATCGT GGCCACCACC
    TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT
    GTGGTCAAGC CTTCTCCAGC CACGGACCTG GATGACCATC TGGGCTTCCT TCCCACCTTC
    GGGCCCTGCT TCGTCAATCT CTATGGCAGC CCCAGGGAAT TCACTGGCTT CCCGGACCCC
    TACGCGGAAC TCAACATGGG CAAGGGGGAA GGCGTGGCCT ACCGAGGTCG GCTTCTGCTC
    TCCCTGGAGA CCAAGCTGGT GGAGCACAGC GGCGAGCAAA GGGTGGAGGA CCTCCCTGCT
    GATGACATCC TTCGGGTGGA GAAATACCTC CGGAGGCGCA AGTACTCTCT CTTTGCCGCC
    TTCTACTCGG CCACCATGCT CCAGGATGTC GACGATGCCA TCCAGTTTGA GGTCAGCATT
    GGGAACTATG GCAACAAGTT CGACACGACC TGTCTGCCAC TGGCCTCCAC CACCCAGTAC
    AGCCGGGCAG TCTTTGATGG GTGCCACTAC TACTACTTGC CCTGGGGCAA TGTGAAGCCT
    GTGGTGGTGC TGTCATCCTA CTGGGAAGAC ATCAGCCACA GAGTCGAGGC TCAGAACCAG
    CTGCTCAGGA TCGCTGACCA TCTGGAAGCT GGCCTGGAGC AAGTCCACTT GGCTTTAAAG
    GCGCAGTTCA ACACAGAGGA TGTGGACTCC CTGTTGGCCC AGCTGATGGA TGAGCTCATC
    TCAGACTGCA GCCAGCCCCT GGGCAATGTC CAAGAGATAC CCTCTGCCAC CCACCTGGAC
    CGGCACCTGT ACAGGCTGCG CACCCGTCAC CTAGGCCAGA TTACTGAGGC GGCCCTGGCT
    CTGAAACTCG GGCATGGCAA GCTCCCTGCC GCCCTGGAGC AGGCTGAAGA CTGGCTCCTG
    CGTCTCCGTG CCCTGGCTGA TGAGCCCCAG AACAGCCTGC CCGACATCAT CATCTGGATG
    CTGCAGGGAA ACAAGCGAGT GGCGTACCAG CGGGTGCCTG CGCACGAGGT CCTCTTCTCC
    CGGCGGGGCG CCAGCTACTG TGGCAAGAAC TGTGGGAAGC TGCAGACGAT ATTTCTGAAA
    TATCCCATGG AGGGGGTGCC CGGAGCCCGG ATGCCAGTAC AAATACGGGT GAAACTCTGG
    TTTGGGCTTT CTGTGGATGA GAAGGAGTTC AACCAGTTTG CTGAGGGAAA GCTGTCCGTC
    TTTGCTGAAA CTTATGAGAA CCAGACCAAA CTGGCCCTGG TTGGGAACTG GGGCACAACA
    GGCCTCACCT ACCCCAAGTT TTCTGATGTC ATGGGCAAGA TCAAGCTACC CAAGGATAGC
    TTCCGCCCCT CAGCTGGCTG GACCTGGGCC GGAGACTGGT TCGTGTGTCC AGAGAAGACC
    CTACTCCATG ACATGGACGC GGGCCACCTG AGTTTTGTGG AGGAGGTGTT TGAGAACCAG
    ACCCGGCTCC CTGGAGGCCA GTGGATCTAC ATGAGTGACA ACTACACAGA TGTGAATGGG
    GATAAGGTGC TTCCTAAGGA TGACATTGAG TGCCCACTGG GCTGGAAGTG GGAAGATGAA
    GAGTGGTCCA CGGACCTCAA TCGGGCCGTC GATGAGCAAG GCTGGGAGTA CAGTATCACC
    ATCCCCCCGG ATCGGAAGCC AAAGCACTGG GTTCCTGCTG AGAAGATGTA TTACACGCAC
    CGTCGGCGGC GCTGGGTCCG CCTGCGCAGA AGGGACCTCA GCCAGATGGA GGCATTGAAG
    AGGCACAGAC AGGCAGAGGC AGAGGGTGAG GGCTGGGAGT ACGCCTCGCT CTTGGGCTGG
    AAGTTCCACC TGGAGTACCG CAAGACTGAT GCTTTCCGCC GCCGCCGCTG GCGCCGCCGC
    ATGGAACCGC TGGAGAAGAC GGGGCCTGCA GCCGTGTTTG CCCTTGAGGG GGCTCTGGGT
    GGTGTGGTAG ATGACAGGAG TGAAGATTCC GTGTCCGTGT CCACCTTGAG CTTTGGAGTG
    AACAGACCCA CGATTTCCTG CATCTTTGAT TATGGGAACC GCTACCACCT ACGCTGCTAC
    ATGTACCAGG CTCGGGACCT GCCCTCGATG GACAAGGACT CTTTTTCTGA TCCCTACGCC
    ATCGTCTCCT TCCTGCACCA GAGCCAGAAG ACAGTGGTGG CGAAGAACAC TCTGAACCCC
    ACCTGGGACC AGACGCTTAT CTTCTATGAG ATTGAGATCT TTGGTGAGCC AGCCAGTATC
    GCTGAGCAGC CGCCCAGCAT TGTGGTGGAG CTGTATGACC ACGACACCTA CGGTGCAGAT
    GAGTTTATGG GACGCTGCAT TTGTCAGCCC AGCCTGGAGC GGACACCCCG GCTGGCCTGG
    TTCCCACTGA TTAGGGGCAG CCAGTCGGCG GGGGAACTGC TGGCCTCTTT TGAGCTCATC
    CAGAGAGAAA AGCCGGCCAT TCACCATATT CCTGGTTTTG AGGTACAGGA TATGTCAGGA
    ATCCTGGAAG AGTCAGAGGA CATAGACCTG CCCTACCCGC CACCTCAGAG GGAGGCCAAC
    ATCTTCATGG TCCCCCAGAA CATCAAGCCT GTTCTCCAGC GTACTGCCAT AGAGATCCTG
    GCGTGGGGCC TGCGGAACAT GAAGAGCTAC CAGCTAGCCA GCATCTCCTC CCCTAGCCTT
    GTGGTAGAGT GCGGGGGCCA GACAGTGCAG TCCTGTGTCA TCAAGAACCT CCGGAAGAAC
    CCCAACTTCG ACATCTGTAC CCTTTTCATG GAAGTGATGC TGCCCAAGGA GGAGGTCTAC
    TGTCCCTCCA TCGTGATCAA GGTCATTGAT AACCGCCAGT TTGGCCGCCG GCCTGTGGTG
    GGCCAGTGTA CCATCCGTTC CCTGGAGAGC TTCATGTGTG ACCTGTACTC CGAGGAGAAT
    CCGTCCCCAC AGGGCGGCCC AGATGATGTG AGTTTACTCA GTCCTGGGGA AGACGTGCTC
    ATTGACATCG ATGACAAGGA GCCCCTCATC CCCATGCAGC TTGCAGATGG TCTGTCGGGC
    TCTGCCCCCA CTAACATGGC ATCTTCTCCA TCCAGTCCTC ATGAGGAAGA GTTCATCGAC
    TGGTGGAGCA AATTCTTTGC CTCCACAGGG GAGAGGGAAA AGTGCGGCTC ATACTTGGAG
    AAGGGCTTTG ACACTCTAAA GGTCTATGAC ACACAACTGG AGAACGTGGA GGCCTTTGAG
    GGCCTGTCTG ACTTTTGTAA CACCTTCAAG CTCTACCGGG GCAAGACTCA GGAGGAGACA
    GAGGACCCAT CTGTTATTGG GGAGTTTAAG GGCCTCTTCA AAATCTATCC CCTCCCAGAA
    GACCCGGCTG TTCCCATGCC CCCGAGACAA TTCCACCAGC TGGCTGCCCA AGGGCCTCAG
    GAGTGCCTGG TCCGCATCTA TATCATCCGA GCATTTGGCC TTCAGCCCAA GGACCCCAAT
    GGGAAGTGTG ATCCTTACAT CAAGATCTCC ATAGGGAAGA AATCAGTGAG TGACCAGGAT
    AACTACATTC CCTGTACTCT GGAGCCTGTG TTTGGAAAGA TGTTCGAGCT GACCTGCACT
    CTGCCTCTGG AGAAGGACCT GAAGATCACA CTCTATGACT ATGACCTCCT CTCCAAAGAT
    GAAAAGATTG GGGAGACAGT CATTGACCTG GAGGATAGGC TGCTGTCCAA GTTTGGGGCT
    CGCTGCGGAC TCCCACAGAC GTACTGTGTC TCTGGACCCA ACCAGTGGAG GGACCAGCTC
    CGCCCCTCCC AGCTTCTCCA CCTCTTCTGC CAGCAGCGCA GACTCAAGGC TCCTGTGTAC
    CTCACGGACC ACGTGGTGTT CCAGGATAAA GAATACAGCA TTGAAGAAAT AGAGGCTGGC
    CGAGTCCCGA ACCCACACCT GGGCCCAGTG GAAGAGCGTC TGGCCTTGCA TGTCCTCCAG
    CAGCAGGGCC TGGTCCCTGA GCATGTGGAG TCCCGGCCTC TCTACAGCCC CCTGCAGCCA
    GACATTGAAC AGGGGAAGCT GCAGATGTGG ATCGACCTAT TTCCCAAGGC CCTGGGGCGT
    CCCGGACCTC CCTTCAACAT CACCCCGCGG AGAGCCAGAA GGTTTTTCCT GCGTTGTATC
    ATCTGGAACA CAAAGGACGT GATCCTGGAC GACCTCAGCA TCACAGGGGA GAAGATGAGT
    GATATTTATG TGAAGGGTTG GATGGTTGGC TTTGAAGAGC ACAAGCAAAA GACAGATGTG
    CATTACCGGT CCCTGGGAGG TGAAGGCAAC TTTAACTGGA GGTTCATTTT CCCCTTTGAC
    TACCTGCCAG CCGAGCAAGT CTGCACCGTC TCTAAGAAGG ATGCTTTCTG GAGGCTGGAC
    AAGACTGAGA GCAAAATCCC AGCACGAGTG GTATTTCAGA TCTGGGACAA TGACAAGTTC
    TCCTTTGACG ACTTTCTGGG CTCCTTGCAG TTGGATCTCA ACCACATGCC CAAGCCTGCC
    AAGACAGCTG AGAAGTGCTC CTTGGACCAG CTGGATGACA CTTTCCACCC GGAGTGGTTT
    GTGTCCCTTT TTGAACAGAA AACAGTTAAG GGCTGGTGGC CCTGCGTAGC AGAAGAGGGC
    GAGAAGAAGA TCCTGGCAGG CAAGCTGGAA ATGACCTTGG AGATCGTAGC AGAGAGTGAG
    CATGAGGAGC GGCCTGCTGG CCAGGGCCGG GATGAACCCA ACATGAACCC CAAGCTCGAG
    GACCCAAGGC GCCCTGACAC CTCCTTCCTC TGGTTCACCT CTCCATACAA GACTATGAAG
    TTCATCCTGT GGCGGCGGTT CCGATGTGCC ATTATCCTCT TCATCATTCT CTTCATCCTG
    CTACTATTCT TAGGCATCTT CATCTATGCT TTCCCGAACT ACGCTGCCAT GAAGCTGGTG
    AAGCCCTTCA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003432270.1
    CDS439..6753
    Translation

    Target ORF information:

    RefSeq Version XM_003432222.3
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris dysferlin (DYSF), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003432222.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC
    CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC
    TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA
    CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGACA CAGGAGGAGA GGAGGACACT
    GAGGACCAGG GGCTCACTGG AGACGAGGGA GAGCCATTAC TGGATCAGAG TGGCGTCCCA
    GGCCCTGGGG CTCCCACCAC CCCGAGGAAG CCACCTTCCC ATCTGCCCCC CTACCACCCT
    GGGAGCAAGA GGAAAAGAAG TGCTCCCGCA GCCAGAAAGC TGCTGTCCGA CAAACCGCAG
    GACTTCCAGA TCAGAGTGCA AGTGATCGAG GGCCGCCAGC TGCCAGGGGT GAATATCAAG
    CCTGTGATCA AGGTCACTGC TGCTGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC
    AGCCCACTAT TTAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTTC GGAGCTGTTT
    GATGAGCCCA TCTTTATCAC GGTCGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT
    GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACATGC TTATCTGAGG
    AAGTGGCTGC TACTCTCCAA CCCTGATGAC TTCTCTGTTG GGGCCAGAGG TTACCTGAAA
    GCAAGCCTTT ATGTGCTGGG GCCCGGAGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT
    GAAGACAAGG AGGACATTGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTGCGAGGA
    GCCCACTTCT GCCTGAAGAT CTTCAGAGCG GAGGACTTAC CACAAATGGA TGATGCCGTG
    ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAATTT GGTAGATCCC
    TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC
    AACCCTCAGT GGAACCAGAG CATCACTCTG CCTGCCATGT TTCCCTCCAT GTGTGAAAAA
    ATGAAGATTC GCGTCATGGA CTGGGACCGC CTTACCCACA ATGACATCGT GGCCACCACC
    TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT
    GTGGTCAAGC CTTCTCCAGC CACGGACCTG GATGACCATC TGGGCTTCCT TCCCACCTTC
    GGGCCCTGCT TCGTCAATCT CTATGGCAGC CCCAGGGAAT TCACTGGCTT CCCGGACCCC
    TACGCGGAAC TCAACATGGG CAAGGGGGAA GGCGTGGCCT ACCGAGGTCG GCTTCTGCTC
    TCCCTGGAGA CCAAGCTGGT GGAGCACAGC GGCGAGCAAA GGGTGGAGGA CCTCCCTGCT
    GATGACATCC TTCGGGTGGA GAAATACCTC CGGAGGCGCA AGTACTCTCT CTTTGCCGCC
    TTCTACTCGG CCACCATGCT CCAGGATGTC GACGATGCCA TCCAGTTTGA GGTCAGCATT
    GGGAACTATG GCAACAAGTT CGACACGACC TGTCTGCCAC TGGCCTCCAC CACCCAGTAC
    AGCCGGGCAG TCTTTGATGG GTGCCACTAC TACTACTTGC CCTGGGGCAA TGTGAAGCCT
    GTGGTGGTGC TGTCATCCTA CTGGGAAGAC ATCAGCCACA GAGTCGAGGC TCAGAACCAG
    CTGCTCAGGA TCGCTGACCA TCTGGAAGCT GGCCTGGAGC AAGTCCACTT GGCTTTAAAG
    GCGCAGTTCA ACACAGAGGA TGTGGACTCC CTGTTGGCCC AGCTGATGGA TGAGCTCATC
    TCAGACTGCA GCCAGCCCCT GGGCAATGTC CAAGAGATAC CCTCTGCCAC CCACCTGGAC
    CGGCACCTGT ACAGGCTGCG CACCCGTCAC CTAGGCCAGA TTACTGAGGC GGCCCTGGCT
    CTGAAACTCG GGCATGGCAA GCTCCCTGCC GCCCTGGAGC AGGCTGAAGA CTGGCTCCTG
    CGTCTCCGTG CCCTGGCTGA TGAGCCCCAG AACAGCCTGC CCGACATCAT CATCTGGATG
    CTGCAGGGAA ACAAGCGAGT GGCGTACCAG CGGGTGCCTG CGCACGAGGT CCTCTTCTCC
    CGGCGGGGCG CCAGCTACTG TGGCAAGAAC TGTGGGAAGC TGCAGACGAT ATTTCTGAAA
    TATCCCATGG AGGGGGTGCC CGGAGCCCGG ATGCCAGTAC AAATACGGGT GAAACTCTGG
    TTTGGGCTTT CTGTGGATGA GAAGGAGTTC AACCAGTTTG CTGAGGGAAA GCTGTCCGTC
    TTTGCTGAAA CTTATGAGAA CCAGACCAAA CTGGCCCTGG TTGGGAACTG GGGCACAACA
    GGCCTCACCT ACCCCAAGTT TTCTGATGTC ATGGGCAAGA TCAAGCTACC CAAGGATAGC
    TTCCGCCCCT CAGCTGGCTG GACCTGGGCC GGAGACTGGT TCGTGTGTCC AGAGAAGACC
    CTACTCCATG ACATGGACGC GGGCCACCTG AGTTTTGTGG AGGAGGTGTT TGAGAACCAG
    ACCCGGCTCC CTGGAGGCCA GTGGATCTAC ATGAGTGACA ACTACACAGA TGTGAATGGG
    GATAAGGTGC TTCCTAAGGA TGACATTGAG TGCCCACTGG GCTGGAAGTG GGAAGATGAA
    GAGTGGTCCA CGGACCTCAA TCGGGCCGTC GATGAGCAAG GCTGGGAGTA CAGTATCACC
    ATCCCCCCGG ATCGGAAGCC AAAGCACTGG GTTCCTGCTG AGAAGATGTA TTACACGCAC
    CGTCGGCGGC GCTGGGTCCG CCTGCGCAGA AGGGACCTCA GCCAGATGGA GGCATTGAAG
    AGGCACAGAC AGGCAGAGGC AGAGGGTGAG GGCTGGGAGT ACGCCTCGCT CTTGGGCTGG
    AAGTTCCACC TGGAGTACCG CAAGACTGAT GCTTTCCGCC GCCGCCGCTG GCGCCGCCGC
    ATGGAACCGC TGGAGAAGAC GGGGCCTGCA GCCGTGTTTG CCCTTGAGGG GGCTCTGGGT
    GGTGTGGTAG ATGACAGGAG TGAAGATTCC GTGTCCGTGT CCACCTTGAG CTTTGGAGTG
    AACAGACCCA CGATTTCCTG CATCTTTGAT TATGGGAACC GCTACCACCT ACGCTGCTAC
    ATGTACCAGG CTCGGGACCT GCCCTCGATG GACAAGGACT CTTTTTCTGA TCCCTACGCC
    ATCGTCTCCT TCCTGCACCA GAGCCAGAAG ACAGTGGTGG CGAAGAACAC TCTGAACCCC
    ACCTGGGACC AGACGCTTAT CTTCTATGAG ATTGAGATCT TTGGTGAGCC AGCCAGTATC
    GCTGAGCAGC CGCCCAGCAT TGTGGTGGAG CTGTATGACC ACGACACCTA CGGTGCAGAT
    GAGTTTATGG GACGCTGCAT TTGTCAGCCC AGCCTGGAGC GGACACCCCG GCTGGCCTGG
    TTCCCACTGA TTAGGGGCAG CCAGTCGGCG GGGGAACTGC TGGCCTCTTT TGAGCTCATC
    CAGAGAGAAA AGCCGGCCAT TCACCATATT CCTGGTTTTG AGGTACAGGA TATGTCAGGA
    ATCCTGGAAG AGTCAGAGGA CATAGACCTG CCCTACCCGC CACCTCAGAG GGAGGCCAAC
    ATCTTCATGG TCCCCCAGAA CATCAAGCCT GTTCTCCAGC GTACTGCCAT AGAGATCCTG
    GCGTGGGGCC TGCGGAACAT GAAGAGCTAC CAGCTAGCCA GCATCTCCTC CCCTAGCCTT
    GTGGTAGAGT GCGGGGGCCA GACAGTGCAG TCCTGTGTCA TCAAGAACCT CCGGAAGAAC
    CCCAACTTCG ACATCTGTAC CCTTTTCATG GAAGTGATGC TGCCCAAGGA GGAGGTCTAC
    TGTCCCTCCA TCGTGATCAA GGTCATTGAT AACCGCCAGT TTGGCCGCCG GCCTGTGGTG
    GGCCAGTGTA CCATCCGTTC CCTGGAGAGC TTCATGTGTG ACCTGTACTC CGAGGAGAAT
    CCGTCCCCAC AGGGCGGCCC AGATGATGTG AGTTTACTCA GTCCTGGGGA AGACGTGCTC
    ATTGACATCG ATGACAAGGA GCCCCTCATC CCCATGCAGC TTGCAGATGG TCTGTCGGGC
    TCTGCCCCCA CTAACATGGC ATCTTCTCCA TCCAGTCCTC ATGAGGAAGA GTTCATCGAC
    TGGTGGAGCA AATTCTTTGC CTCCACAGGG GAGAGGGAAA AGTGCGGCTC ATACTTGGAG
    AAGGGCTTTG ACACTCTAAA GGTCTATGAC ACACAACTGG AGAACGTGGA GGCCTTTGAG
    GGCCTGTCTG ACTTTTGTAA CACCTTCAAG CTCTACCGGG GCAAGACTCA GGAGGAGACA
    GAGGACCCAT CTGTTATTGG GGAGTTTAAG GGCCTCTTCA AAATCTATCC CCTCCCAGAA
    GACCCGGCTG TTCCCATGCC CCCGAGACAA TTCCACCAGC TGGCTGCCCA AGGGCCTCAG
    GAGTGCCTGG TCCGCATCTA TATCATCCGA GCATTTGGCC TTCAGCCCAA GGACCCCAAT
    GGGAAGTGTG ATCCTTACAT CAAGATCTCC ATAGGGAAGA AATCAGTGAG TGACCAGGAT
    AACTACATTC CCTGTACTCT GGAGCCTGTG TTTGGAAAGA TGTTCGAGCT GACCTGCACT
    CTGCCTCTGG AGAAGGACCT GAAGATCACA CTCTATGACT ATGACCTCCT CTCCAAAGAT
    GAAAAGATTG GGGAGACAGT CATTGACCTG GAGGATAGGC TGCTGTCCAA GTTTGGGGCT
    CGCTGCGGAC TCCCACAGAC GTACTGTGTC TCTGGACCCA ACCAGTGGAG GGACCAGCTC
    CGCCCCTCCC AGCTTCTCCA CCTCTTCTGC CAGCAGCGCA GACTCAAGGC TCCTGTGTAC
    CTCACGGACC ACGTGGTGTT CCAGGATAAA GAATACAGCA TTGAAGAAAT AGAGGCTGGC
    CGAGTCCCGA ACCCACACCT GGGCCCAGTG GAAGAGCGTC TGGCCTTGCA TGTCCTCCAG
    CAGCAGGGCC TGGTCCCTGA GCATGTGGAG TCCCGGCCTC TCTACAGCCC CCTGCAGCCA
    GACATTGAAC AGGGGAAGCT GCAGATGTGG ATCGACCTAT TTCCCAAGGC CCTGGGGCGT
    CCCGGACCTC CCTTCAACAT CACCCCGCGG AGAGCCAGAA GGTTTTTCCT GCGTTGTATC
    ATCTGGAACA CAAAGGACGT GATCCTGGAC GACCTCAGCA TCACAGGGGA GAAGATGAGT
    GATATTTATG TGAAGGGTTG GATGGTTGGC TTTGAAGAGC ACAAGCAAAA GACAGATGTG
    CATTACCGGT CCCTGGGAGG TGAAGGCAAC TTTAACTGGA GGTTCATTTT CCCCTTTGAC
    TACCTGCCAG CCGAGCAAGT CTGCACCGTC TCTAAGAAGG ATGCTTTCTG GAGGCTGGAC
    AAGACTGAGA GCAAAATCCC AGCACGAGTG GTATTTCAGA TCTGGGACAA TGACAAGTTC
    TCCTTTGACG ACTTTCTGGG CTCCTTGCAG TTGGATCTCA ACCACATGCC CAAGCCTGCC
    AAGACAGCTG AGAAGTGCTC CTTGGACCAG CTGGATGACA CTTTCCACCC GGAGTGGTTT
    GTGTCCCTTT TTGAACAGAA AACAGTTAAG GGCTGGTGGC CCTGCGTAGC AGAAGAGGGC
    GAGAAGAAGA TCCTGGCAGG CAAGCTGGAA ATGACCTTGG AGATCGTAGC AGAGAGTGAG
    CATGAGGAGC GGCCTGCTGG CCAGGGCCGG GATGAACCCA ACATGAACCC CAAGCTCGAG
    GACCCAAGGC GCCCTGACAC CTCCTTCCTC TGGTTCACCT CTCCATACAA GACTATGAAG
    TTCATCCTGT GGCGGCGGTT CCGATGTGCC ATTATCCTCT TCATCATTCT CTTCATCCTG
    CTACTATTCT TAGGCATCTT CATCTATGCT TTCCCGAACT ACGCTGCCAT GAAGCTGGTG
    AAGCCCTTCA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe19211
    Clone ID Related Accession (Same CDS sequence) XM_003432222.3 , XM_003432222.4
    Accession Version XM_003432222.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6315bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product dysferlin isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003432222.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC
    CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC
    TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA
    CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGACA CAGGAGGAGA GGAGGACACT
    GAGGACCAGG GGCTCACTGG AGACGAGGGA GAGCCATTAC TGGATCAGAG TGGCGTCCCA
    GGCCCTGGGG CTCCCACCAC CCCGAGGAAG CCACCTTCCC ATCTGCCCCC CTACCACCCT
    GGGAGCAAGA GGAAAAGAAG TGCTCCCGCA GCCAGAAAGC TGCTGTCCGA CAAACCGCAG
    GACTTCCAGA TCAGAGTGCA AGTGATCGAG GGCCGCCAGC TGCCAGGGGT GAATATCAAG
    CCTGTGATCA AGGTCACTGC TGCTGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC
    AGCCCACTAT TTAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTTC GGAGCTGTTT
    GATGAGCCCA TCTTTATCAC GGTCGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT
    GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACATGC TTATCTGAGG
    AAGTGGCTGC TACTCTCCAA CCCTGATGAC TTCTCTGTTG GGGCCAGAGG TTACCTGAAA
    GCAAGCCTTT ATGTGCTGGG GCCCGGAGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT
    GAAGACAAGG AGGACATTGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTGCGAGGA
    GCCCACTTCT GCCTGAAGAT CTTCAGAGCG GAGGACTTAC CACAAATGGA TGATGCCGTG
    ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAATTT GGTAGATCCC
    TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC
    AACCCTCAGT GGAACCAGAG CATCACTCTG CCTGCCATGT TTCCCTCCAT GTGTGAAAAA
    ATGAAGATTC GCGTCATGGA CTGGGACCGC CTTACCCACA ATGACATCGT GGCCACCACC
    TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT
    GTGGTCAAGC CTTCTCCAGC CACGGACCTG GATGACCATC TGGGCTTCCT TCCCACCTTC
    GGGCCCTGCT TCGTCAATCT CTATGGCAGC CCCAGGGAAT TCACTGGCTT CCCGGACCCC
    TACGCGGAAC TCAACATGGG CAAGGGGGAA GGCGTGGCCT ACCGAGGTCG GCTTCTGCTC
    TCCCTGGAGA CCAAGCTGGT GGAGCACAGC GGCGAGCAAA GGGTGGAGGA CCTCCCTGCT
    GATGACATCC TTCGGGTGGA GAAATACCTC CGGAGGCGCA AGTACTCTCT CTTTGCCGCC
    TTCTACTCGG CCACCATGCT CCAGGATGTC GACGATGCCA TCCAGTTTGA GGTCAGCATT
    GGGAACTATG GCAACAAGTT CGACACGACC TGTCTGCCAC TGGCCTCCAC CACCCAGTAC
    AGCCGGGCAG TCTTTGATGG GTGCCACTAC TACTACTTGC CCTGGGGCAA TGTGAAGCCT
    GTGGTGGTGC TGTCATCCTA CTGGGAAGAC ATCAGCCACA GAGTCGAGGC TCAGAACCAG
    CTGCTCAGGA TCGCTGACCA TCTGGAAGCT GGCCTGGAGC AAGTCCACTT GGCTTTAAAG
    GCGCAGTTCA ACACAGAGGA TGTGGACTCC CTGTTGGCCC AGCTGATGGA TGAGCTCATC
    TCAGACTGCA GCCAGCCCCT GGGCAATGTC CAAGAGATAC CCTCTGCCAC CCACCTGGAC
    CGGCACCTGT ACAGGCTGCG CACCCGTCAC CTAGGCCAGA TTACTGAGGC GGCCCTGGCT
    CTGAAACTCG GGCATGGCAA GCTCCCTGCC GCCCTGGAGC AGGCTGAAGA CTGGCTCCTG
    CGTCTCCGTG CCCTGGCTGA TGAGCCCCAG AACAGCCTGC CCGACATCAT CATCTGGATG
    CTGCAGGGAA ACAAGCGAGT GGCGTACCAG CGGGTGCCTG CGCACGAGGT CCTCTTCTCC
    CGGCGGGGCG CCAGCTACTG TGGCAAGAAC TGTGGGAAGC TGCAGACGAT ATTTCTGAAA
    TATCCCATGG AGGGGGTGCC CGGAGCCCGG ATGCCAGTAC AAATACGGGT GAAACTCTGG
    TTTGGGCTTT CTGTGGATGA GAAGGAGTTC AACCAGTTTG CTGAGGGAAA GCTGTCCGTC
    TTTGCTGAAA CTTATGAGAA CCAGACCAAA CTGGCCCTGG TTGGGAACTG GGGCACAACA
    GGCCTCACCT ACCCCAAGTT TTCTGATGTC ATGGGCAAGA TCAAGCTACC CAAGGATAGC
    TTCCGCCCCT CAGCTGGCTG GACCTGGGCC GGAGACTGGT TCGTGTGTCC AGAGAAGACC
    CTACTCCATG ACATGGACGC GGGCCACCTG AGTTTTGTGG AGGAGGTGTT TGAGAACCAG
    ACCCGGCTCC CTGGAGGCCA GTGGATCTAC ATGAGTGACA ACTACACAGA TGTGAATGGG
    GATAAGGTGC TTCCTAAGGA TGACATTGAG TGCCCACTGG GCTGGAAGTG GGAAGATGAA
    GAGTGGTCCA CGGACCTCAA TCGGGCCGTC GATGAGCAAG GCTGGGAGTA CAGTATCACC
    ATCCCCCCGG ATCGGAAGCC AAAGCACTGG GTTCCTGCTG AGAAGATGTA TTACACGCAC
    CGTCGGCGGC GCTGGGTCCG CCTGCGCAGA AGGGACCTCA GCCAGATGGA GGCATTGAAG
    AGGCACAGAC AGGCAGAGGC AGAGGGTGAG GGCTGGGAGT ACGCCTCGCT CTTGGGCTGG
    AAGTTCCACC TGGAGTACCG CAAGACTGAT GCTTTCCGCC GCCGCCGCTG GCGCCGCCGC
    ATGGAACCGC TGGAGAAGAC GGGGCCTGCA GCCGTGTTTG CCCTTGAGGG GGCTCTGGGT
    GGTGTGGTAG ATGACAGGAG TGAAGATTCC GTGTCCGTGT CCACCTTGAG CTTTGGAGTG
    AACAGACCCA CGATTTCCTG CATCTTTGAT TATGGGAACC GCTACCACCT ACGCTGCTAC
    ATGTACCAGG CTCGGGACCT GCCCTCGATG GACAAGGACT CTTTTTCTGA TCCCTACGCC
    ATCGTCTCCT TCCTGCACCA GAGCCAGAAG ACAGTGGTGG CGAAGAACAC TCTGAACCCC
    ACCTGGGACC AGACGCTTAT CTTCTATGAG ATTGAGATCT TTGGTGAGCC AGCCAGTATC
    GCTGAGCAGC CGCCCAGCAT TGTGGTGGAG CTGTATGACC ACGACACCTA CGGTGCAGAT
    GAGTTTATGG GACGCTGCAT TTGTCAGCCC AGCCTGGAGC GGACACCCCG GCTGGCCTGG
    TTCCCACTGA TTAGGGGCAG CCAGTCGGCG GGGGAACTGC TGGCCTCTTT TGAGCTCATC
    CAGAGAGAAA AGCCGGCCAT TCACCATATT CCTGGTTTTG AGGTACAGGA TATGTCAGGA
    ATCCTGGAAG AGTCAGAGGA CATAGACCTG CCCTACCCGC CACCTCAGAG GGAGGCCAAC
    ATCTTCATGG TCCCCCAGAA CATCAAGCCT GTTCTCCAGC GTACTGCCAT AGAGATCCTG
    GCGTGGGGCC TGCGGAACAT GAAGAGCTAC CAGCTAGCCA GCATCTCCTC CCCTAGCCTT
    GTGGTAGAGT GCGGGGGCCA GACAGTGCAG TCCTGTGTCA TCAAGAACCT CCGGAAGAAC
    CCCAACTTCG ACATCTGTAC CCTTTTCATG GAAGTGATGC TGCCCAAGGA GGAGGTCTAC
    TGTCCCTCCA TCGTGATCAA GGTCATTGAT AACCGCCAGT TTGGCCGCCG GCCTGTGGTG
    GGCCAGTGTA CCATCCGTTC CCTGGAGAGC TTCATGTGTG ACCTGTACTC CGAGGAGAAT
    CCGTCCCCAC AGGGCGGCCC AGATGATGTG AGTTTACTCA GTCCTGGGGA AGACGTGCTC
    ATTGACATCG ATGACAAGGA GCCCCTCATC CCCATGCAGC TTGCAGATGG TCTGTCGGGC
    TCTGCCCCCA CTAACATGGC ATCTTCTCCA TCCAGTCCTC ATGAGGAAGA GTTCATCGAC
    TGGTGGAGCA AATTCTTTGC CTCCACAGGG GAGAGGGAAA AGTGCGGCTC ATACTTGGAG
    AAGGGCTTTG ACACTCTAAA GGTCTATGAC ACACAACTGG AGAACGTGGA GGCCTTTGAG
    GGCCTGTCTG ACTTTTGTAA CACCTTCAAG CTCTACCGGG GCAAGACTCA GGAGGAGACA
    GAGGACCCAT CTGTTATTGG GGAGTTTAAG GGCCTCTTCA AAATCTATCC CCTCCCAGAA
    GACCCGGCTG TTCCCATGCC CCCGAGACAA TTCCACCAGC TGGCTGCCCA AGGGCCTCAG
    GAGTGCCTGG TCCGCATCTA TATCATCCGA GCATTTGGCC TTCAGCCCAA GGACCCCAAT
    GGGAAGTGTG ATCCTTACAT CAAGATCTCC ATAGGGAAGA AATCAGTGAG TGACCAGGAT
    AACTACATTC CCTGTACTCT GGAGCCTGTG TTTGGAAAGA TGTTCGAGCT GACCTGCACT
    CTGCCTCTGG AGAAGGACCT GAAGATCACA CTCTATGACT ATGACCTCCT CTCCAAAGAT
    GAAAAGATTG GGGAGACAGT CATTGACCTG GAGGATAGGC TGCTGTCCAA GTTTGGGGCT
    CGCTGCGGAC TCCCACAGAC GTACTGTGTC TCTGGACCCA ACCAGTGGAG GGACCAGCTC
    CGCCCCTCCC AGCTTCTCCA CCTCTTCTGC CAGCAGCGCA GACTCAAGGC TCCTGTGTAC
    CTCACGGACC ACGTGGTGTT CCAGGATAAA GAATACAGCA TTGAAGAAAT AGAGGCTGGC
    CGAGTCCCGA ACCCACACCT GGGCCCAGTG GAAGAGCGTC TGGCCTTGCA TGTCCTCCAG
    CAGCAGGGCC TGGTCCCTGA GCATGTGGAG TCCCGGCCTC TCTACAGCCC CCTGCAGCCA
    GACATTGAAC AGGGGAAGCT GCAGATGTGG ATCGACCTAT TTCCCAAGGC CCTGGGGCGT
    CCCGGACCTC CCTTCAACAT CACCCCGCGG AGAGCCAGAA GGTTTTTCCT GCGTTGTATC
    ATCTGGAACA CAAAGGACGT GATCCTGGAC GACCTCAGCA TCACAGGGGA GAAGATGAGT
    GATATTTATG TGAAGGGTTG GATGGTTGGC TTTGAAGAGC ACAAGCAAAA GACAGATGTG
    CATTACCGGT CCCTGGGAGG TGAAGGCAAC TTTAACTGGA GGTTCATTTT CCCCTTTGAC
    TACCTGCCAG CCGAGCAAGT CTGCACCGTC TCTAAGAAGG ATGCTTTCTG GAGGCTGGAC
    AAGACTGAGA GCAAAATCCC AGCACGAGTG GTATTTCAGA TCTGGGACAA TGACAAGTTC
    TCCTTTGACG ACTTTCTGGG CTCCTTGCAG TTGGATCTCA ACCACATGCC CAAGCCTGCC
    AAGACAGCTG AGAAGTGCTC CTTGGACCAG CTGGATGACA CTTTCCACCC GGAGTGGTTT
    GTGTCCCTTT TTGAACAGAA AACAGTTAAG GGCTGGTGGC CCTGCGTAGC AGAAGAGGGC
    GAGAAGAAGA TCCTGGCAGG CAAGCTGGAA ATGACCTTGG AGATCGTAGC AGAGAGTGAG
    CATGAGGAGC GGCCTGCTGG CCAGGGCCGG GATGAACCCA ACATGAACCC CAAGCTCGAG
    GACCCAAGGC GCCCTGACAC CTCCTTCCTC TGGTTCACCT CTCCATACAA GACTATGAAG
    TTCATCCTGT GGCGGCGGTT CCGATGTGCC ATTATCCTCT TCATCATTCT CTTCATCCTG
    CTACTATTCT TAGGCATCTT CATCTATGCT TTCCCGAACT ACGCTGCCAT GAAGCTGGTG
    AAGCCCTTCA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003432270.1
    CDS405..6719
    Translation

    Target ORF information:

    RefSeq Version XM_003432222.4
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris dysferlin (DYSF), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003432222.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC
    CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC
    TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA
    CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGACA CAGGAGGAGA GGAGGACACT
    GAGGACCAGG GGCTCACTGG AGACGAGGGA GAGCCATTAC TGGATCAGAG TGGCGTCCCA
    GGCCCTGGGG CTCCCACCAC CCCGAGGAAG CCACCTTCCC ATCTGCCCCC CTACCACCCT
    GGGAGCAAGA GGAAAAGAAG TGCTCCCGCA GCCAGAAAGC TGCTGTCCGA CAAACCGCAG
    GACTTCCAGA TCAGAGTGCA AGTGATCGAG GGCCGCCAGC TGCCAGGGGT GAATATCAAG
    CCTGTGATCA AGGTCACTGC TGCTGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC
    AGCCCACTAT TTAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTTC GGAGCTGTTT
    GATGAGCCCA TCTTTATCAC GGTCGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT
    GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACATGC TTATCTGAGG
    AAGTGGCTGC TACTCTCCAA CCCTGATGAC TTCTCTGTTG GGGCCAGAGG TTACCTGAAA
    GCAAGCCTTT ATGTGCTGGG GCCCGGAGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT
    GAAGACAAGG AGGACATTGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTGCGAGGA
    GCCCACTTCT GCCTGAAGAT CTTCAGAGCG GAGGACTTAC CACAAATGGA TGATGCCGTG
    ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAATTT GGTAGATCCC
    TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC
    AACCCTCAGT GGAACCAGAG CATCACTCTG CCTGCCATGT TTCCCTCCAT GTGTGAAAAA
    ATGAAGATTC GCGTCATGGA CTGGGACCGC CTTACCCACA ATGACATCGT GGCCACCACC
    TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT
    GTGGTCAAGC CTTCTCCAGC CACGGACCTG GATGACCATC TGGGCTTCCT TCCCACCTTC
    GGGCCCTGCT TCGTCAATCT CTATGGCAGC CCCAGGGAAT TCACTGGCTT CCCGGACCCC
    TACGCGGAAC TCAACATGGG CAAGGGGGAA GGCGTGGCCT ACCGAGGTCG GCTTCTGCTC
    TCCCTGGAGA CCAAGCTGGT GGAGCACAGC GGCGAGCAAA GGGTGGAGGA CCTCCCTGCT
    GATGACATCC TTCGGGTGGA GAAATACCTC CGGAGGCGCA AGTACTCTCT CTTTGCCGCC
    TTCTACTCGG CCACCATGCT CCAGGATGTC GACGATGCCA TCCAGTTTGA GGTCAGCATT
    GGGAACTATG GCAACAAGTT CGACACGACC TGTCTGCCAC TGGCCTCCAC CACCCAGTAC
    AGCCGGGCAG TCTTTGATGG GTGCCACTAC TACTACTTGC CCTGGGGCAA TGTGAAGCCT
    GTGGTGGTGC TGTCATCCTA CTGGGAAGAC ATCAGCCACA GAGTCGAGGC TCAGAACCAG
    CTGCTCAGGA TCGCTGACCA TCTGGAAGCT GGCCTGGAGC AAGTCCACTT GGCTTTAAAG
    GCGCAGTTCA ACACAGAGGA TGTGGACTCC CTGTTGGCCC AGCTGATGGA TGAGCTCATC
    TCAGACTGCA GCCAGCCCCT GGGCAATGTC CAAGAGATAC CCTCTGCCAC CCACCTGGAC
    CGGCACCTGT ACAGGCTGCG CACCCGTCAC CTAGGCCAGA TTACTGAGGC GGCCCTGGCT
    CTGAAACTCG GGCATGGCAA GCTCCCTGCC GCCCTGGAGC AGGCTGAAGA CTGGCTCCTG
    CGTCTCCGTG CCCTGGCTGA TGAGCCCCAG AACAGCCTGC CCGACATCAT CATCTGGATG
    CTGCAGGGAA ACAAGCGAGT GGCGTACCAG CGGGTGCCTG CGCACGAGGT CCTCTTCTCC
    CGGCGGGGCG CCAGCTACTG TGGCAAGAAC TGTGGGAAGC TGCAGACGAT ATTTCTGAAA
    TATCCCATGG AGGGGGTGCC CGGAGCCCGG ATGCCAGTAC AAATACGGGT GAAACTCTGG
    TTTGGGCTTT CTGTGGATGA GAAGGAGTTC AACCAGTTTG CTGAGGGAAA GCTGTCCGTC
    TTTGCTGAAA CTTATGAGAA CCAGACCAAA CTGGCCCTGG TTGGGAACTG GGGCACAACA
    GGCCTCACCT ACCCCAAGTT TTCTGATGTC ATGGGCAAGA TCAAGCTACC CAAGGATAGC
    TTCCGCCCCT CAGCTGGCTG GACCTGGGCC GGAGACTGGT TCGTGTGTCC AGAGAAGACC
    CTACTCCATG ACATGGACGC GGGCCACCTG AGTTTTGTGG AGGAGGTGTT TGAGAACCAG
    ACCCGGCTCC CTGGAGGCCA GTGGATCTAC ATGAGTGACA ACTACACAGA TGTGAATGGG
    GATAAGGTGC TTCCTAAGGA TGACATTGAG TGCCCACTGG GCTGGAAGTG GGAAGATGAA
    GAGTGGTCCA CGGACCTCAA TCGGGCCGTC GATGAGCAAG GCTGGGAGTA CAGTATCACC
    ATCCCCCCGG ATCGGAAGCC AAAGCACTGG GTTCCTGCTG AGAAGATGTA TTACACGCAC
    CGTCGGCGGC GCTGGGTCCG CCTGCGCAGA AGGGACCTCA GCCAGATGGA GGCATTGAAG
    AGGCACAGAC AGGCAGAGGC AGAGGGTGAG GGCTGGGAGT ACGCCTCGCT CTTGGGCTGG
    AAGTTCCACC TGGAGTACCG CAAGACTGAT GCTTTCCGCC GCCGCCGCTG GCGCCGCCGC
    ATGGAACCGC TGGAGAAGAC GGGGCCTGCA GCCGTGTTTG CCCTTGAGGG GGCTCTGGGT
    GGTGTGGTAG ATGACAGGAG TGAAGATTCC GTGTCCGTGT CCACCTTGAG CTTTGGAGTG
    AACAGACCCA CGATTTCCTG CATCTTTGAT TATGGGAACC GCTACCACCT ACGCTGCTAC
    ATGTACCAGG CTCGGGACCT GCCCTCGATG GACAAGGACT CTTTTTCTGA TCCCTACGCC
    ATCGTCTCCT TCCTGCACCA GAGCCAGAAG ACAGTGGTGG CGAAGAACAC TCTGAACCCC
    ACCTGGGACC AGACGCTTAT CTTCTATGAG ATTGAGATCT TTGGTGAGCC AGCCAGTATC
    GCTGAGCAGC CGCCCAGCAT TGTGGTGGAG CTGTATGACC ACGACACCTA CGGTGCAGAT
    GAGTTTATGG GACGCTGCAT TTGTCAGCCC AGCCTGGAGC GGACACCCCG GCTGGCCTGG
    TTCCCACTGA TTAGGGGCAG CCAGTCGGCG GGGGAACTGC TGGCCTCTTT TGAGCTCATC
    CAGAGAGAAA AGCCGGCCAT TCACCATATT CCTGGTTTTG AGGTACAGGA TATGTCAGGA
    ATCCTGGAAG AGTCAGAGGA CATAGACCTG CCCTACCCGC CACCTCAGAG GGAGGCCAAC
    ATCTTCATGG TCCCCCAGAA CATCAAGCCT GTTCTCCAGC GTACTGCCAT AGAGATCCTG
    GCGTGGGGCC TGCGGAACAT GAAGAGCTAC CAGCTAGCCA GCATCTCCTC CCCTAGCCTT
    GTGGTAGAGT GCGGGGGCCA GACAGTGCAG TCCTGTGTCA TCAAGAACCT CCGGAAGAAC
    CCCAACTTCG ACATCTGTAC CCTTTTCATG GAAGTGATGC TGCCCAAGGA GGAGGTCTAC
    TGTCCCTCCA TCGTGATCAA GGTCATTGAT AACCGCCAGT TTGGCCGCCG GCCTGTGGTG
    GGCCAGTGTA CCATCCGTTC CCTGGAGAGC TTCATGTGTG ACCTGTACTC CGAGGAGAAT
    CCGTCCCCAC AGGGCGGCCC AGATGATGTG AGTTTACTCA GTCCTGGGGA AGACGTGCTC
    ATTGACATCG ATGACAAGGA GCCCCTCATC CCCATGCAGC TTGCAGATGG TCTGTCGGGC
    TCTGCCCCCA CTAACATGGC ATCTTCTCCA TCCAGTCCTC ATGAGGAAGA GTTCATCGAC
    TGGTGGAGCA AATTCTTTGC CTCCACAGGG GAGAGGGAAA AGTGCGGCTC ATACTTGGAG
    AAGGGCTTTG ACACTCTAAA GGTCTATGAC ACACAACTGG AGAACGTGGA GGCCTTTGAG
    GGCCTGTCTG ACTTTTGTAA CACCTTCAAG CTCTACCGGG GCAAGACTCA GGAGGAGACA
    GAGGACCCAT CTGTTATTGG GGAGTTTAAG GGCCTCTTCA AAATCTATCC CCTCCCAGAA
    GACCCGGCTG TTCCCATGCC CCCGAGACAA TTCCACCAGC TGGCTGCCCA AGGGCCTCAG
    GAGTGCCTGG TCCGCATCTA TATCATCCGA GCATTTGGCC TTCAGCCCAA GGACCCCAAT
    GGGAAGTGTG ATCCTTACAT CAAGATCTCC ATAGGGAAGA AATCAGTGAG TGACCAGGAT
    AACTACATTC CCTGTACTCT GGAGCCTGTG TTTGGAAAGA TGTTCGAGCT GACCTGCACT
    CTGCCTCTGG AGAAGGACCT GAAGATCACA CTCTATGACT ATGACCTCCT CTCCAAAGAT
    GAAAAGATTG GGGAGACAGT CATTGACCTG GAGGATAGGC TGCTGTCCAA GTTTGGGGCT
    CGCTGCGGAC TCCCACAGAC GTACTGTGTC TCTGGACCCA ACCAGTGGAG GGACCAGCTC
    CGCCCCTCCC AGCTTCTCCA CCTCTTCTGC CAGCAGCGCA GACTCAAGGC TCCTGTGTAC
    CTCACGGACC ACGTGGTGTT CCAGGATAAA GAATACAGCA TTGAAGAAAT AGAGGCTGGC
    CGAGTCCCGA ACCCACACCT GGGCCCAGTG GAAGAGCGTC TGGCCTTGCA TGTCCTCCAG
    CAGCAGGGCC TGGTCCCTGA GCATGTGGAG TCCCGGCCTC TCTACAGCCC CCTGCAGCCA
    GACATTGAAC AGGGGAAGCT GCAGATGTGG ATCGACCTAT TTCCCAAGGC CCTGGGGCGT
    CCCGGACCTC CCTTCAACAT CACCCCGCGG AGAGCCAGAA GGTTTTTCCT GCGTTGTATC
    ATCTGGAACA CAAAGGACGT GATCCTGGAC GACCTCAGCA TCACAGGGGA GAAGATGAGT
    GATATTTATG TGAAGGGTTG GATGGTTGGC TTTGAAGAGC ACAAGCAAAA GACAGATGTG
    CATTACCGGT CCCTGGGAGG TGAAGGCAAC TTTAACTGGA GGTTCATTTT CCCCTTTGAC
    TACCTGCCAG CCGAGCAAGT CTGCACCGTC TCTAAGAAGG ATGCTTTCTG GAGGCTGGAC
    AAGACTGAGA GCAAAATCCC AGCACGAGTG GTATTTCAGA TCTGGGACAA TGACAAGTTC
    TCCTTTGACG ACTTTCTGGG CTCCTTGCAG TTGGATCTCA ACCACATGCC CAAGCCTGCC
    AAGACAGCTG AGAAGTGCTC CTTGGACCAG CTGGATGACA CTTTCCACCC GGAGTGGTTT
    GTGTCCCTTT TTGAACAGAA AACAGTTAAG GGCTGGTGGC CCTGCGTAGC AGAAGAGGGC
    GAGAAGAAGA TCCTGGCAGG CAAGCTGGAA ATGACCTTGG AGATCGTAGC AGAGAGTGAG
    CATGAGGAGC GGCCTGCTGG CCAGGGCCGG GATGAACCCA ACATGAACCC CAAGCTCGAG
    GACCCAAGGC GCCCTGACAC CTCCTTCCTC TGGTTCACCT CTCCATACAA GACTATGAAG
    TTCATCCTGT GGCGGCGGTT CCGATGTGCC ATTATCCTCT TCATCATTCT CTTCATCCTG
    CTACTATTCT TAGGCATCTT CATCTATGCT TTCCCGAACT ACGCTGCCAT GAAGCTGGTG
    AAGCCCTTCA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe19212
    Clone ID Related Accession (Same CDS sequence) XM_003432233.3 , XM_003432233.4
    Accession Version XM_003432233.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6306bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product dysferlin isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003432233.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG
    CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC
    GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG
    AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT
    CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG
    ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT
    GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT
    TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC
    GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT
    ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT
    GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC
    GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC
    AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC
    AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC
    ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG
    GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT
    GCCCCTGGAG GAGAAATAGA AGTGGATGAC CATCTGGGCT TCCTTCCCAC CTTCGGGCCC
    TGCTTCGTCA ATCTCTATGG CAGCCCCAGG GAATTCACTG GCTTCCCGGA CCCCTACGCG
    GAACTCAACA TGGGCAAGGG GGAAGGCGTG GCCTACCGAG GTCGGCTTCT GCTCTCCCTG
    GAGACCAAGC TGGTGGAGCA CAGCGGCGAG CAAAGGGTGG AGGACCTCCC TGCTGATGAC
    ATCCTTCGGG TGGAGAAATA CCTCCGGAGG CGCAAGTACT CTCTCTTTGC CGCCTTCTAC
    TCGGCCACCA TGCTCCAGGA TGTCGACGAT GCCATCCAGT TTGAGGTCAG CATTGGGAAC
    TATGGCAACA AGTTCGACAC GACCTGTCTG CCACTGGCCT CCACCACCCA GTACAGCCGG
    GCAGTCTTTG ATGGGTGCCA CTACTACTAC TTGCCCTGGG GCAATGTGAA GCCTGTGGTG
    GTGCTGTCAT CCTACTGGGA AGACATCAGC CACAGAGTCG AGGCTCAGAA CCAGCTGCTC
    AGGATCGCTG ACCATCTGGA AGCTGGCCTG GAGCAAGTCC ACTTGGCTTT AAAGGCGCAG
    TTCAACACAG AGGATGTGGA CTCCCTGTTG GCCCAGCTGA TGGATGAGCT CATCTCAGAC
    TGCAGCCAGC CCCTGGGCAA TGTCCAAGAG ATACCCTCTG CCACCCACCT GGACCGGCAC
    CTGTACAGGC TGCGCACCCG TCACCTAGGC CAGATTACTG AGGCGGCCCT GGCTCTGAAA
    CTCGGGCATG GCAAGCTCCC TGCCGCCCTG GAGCAGGCTG AAGACTGGCT CCTGCGTCTC
    CGTGCCCTGG CTGATGAGCC CCAGAACAGC CTGCCCGACA TCATCATCTG GATGCTGCAG
    GGAAACAAGC GAGTGGCGTA CCAGCGGGTG CCTGCGCACG AGGTCCTCTT CTCCCGGCGG
    GGCGCCAGCT ACTGTGGCAA GAACTGTGGG AAGCTGCAGA CGATATTTCT GAAATATCCC
    ATGGAGGGGG TGCCCGGAGC CCGGATGCCA GTACAAATAC GGGTGAAACT CTGGTTTGGG
    CTTTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GAAAGCTGTC CGTCTTTGCT
    GAAACTTATG AGAACCAGAC CAAACTGGCC CTGGTTGGGA ACTGGGGCAC AACAGGCCTC
    ACCTACCCCA AGTTTTCTGA TGTCATGGGC AAGATCAAGC TACCCAAGGA TAGCTTCCGC
    CCCTCAGCTG GCTGGACCTG GGCCGGAGAC TGGTTCGTGT GTCCAGAGAA GACCCTACTC
    CATGACATGG ACGCGGGCCA CCTGAGTTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG
    CTCCCTGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CAGATGTGAA TGGGGATAAG
    GTGCTTCCTA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAAGAGTGG
    TCCACGGACC TCAATCGGGC CGTCGATGAG CAAGGCTGGG AGTACAGTAT CACCATCCCC
    CCGGATCGGA AGCCAAAGCA CTGGGTTCCT GCTGAGAAGA TGTATTACAC GCACCGTCGG
    CGGCGCTGGG TCCGCCTGCG CAGAAGGGAC CTCAGCCAGA TGGAGGCATT GAAGAGGCAC
    AGACAGGCAG AGGCAGAGGG TGAGGGCTGG GAGTACGCCT CGCTCTTGGG CTGGAAGTTC
    CACCTGGAGT ACCGCAAGAC TGATGCTTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAA
    CCGCTGGAGA AGACGGGGCC TGCAGCCGTG TTTGCCCTTG AGGGGGCTCT GGGTGGTGTG
    GTAGATGACA GGAGTGAAGA TTCCGTGTCC GTGTCCACCT TGAGCTTTGG AGTGAACAGA
    CCCACGATTT CCTGCATCTT TGATTATGGG AACCGCTACC ACCTACGCTG CTACATGTAC
    CAGGCTCGGG ACCTGCCCTC GATGGACAAG GACTCTTTTT CTGATCCCTA CGCCATCGTC
    TCCTTCCTGC ACCAGAGCCA GAAGACAGTG GTGGCGAAGA ACACTCTGAA CCCCACCTGG
    GACCAGACGC TTATCTTCTA TGAGATTGAG ATCTTTGGTG AGCCAGCCAG TATCGCTGAG
    CAGCCGCCCA GCATTGTGGT GGAGCTGTAT GACCACGACA CCTACGGTGC AGATGAGTTT
    ATGGGACGCT GCATTTGTCA GCCCAGCCTG GAGCGGACAC CCCGGCTGGC CTGGTTCCCA
    CTGATTAGGG GCAGCCAGTC GGCGGGGGAA CTGCTGGCCT CTTTTGAGCT CATCCAGAGA
    GAAAAGCCGG CCATTCACCA TATTCCTGGT TTTGAGGTAC AGGATATGTC AGGAATCCTG
    GAAGAGTCAG AGGACATAGA CCTGCCCTAC CCGCCACCTC AGAGGGAGGC CAACATCTTC
    ATGGTCCCCC AGAACATCAA GCCTGTTCTC CAGCGTACTG CCATAGAGAT CCTGGCGTGG
    GGCCTGCGGA ACATGAAGAG CTACCAGCTA GCCAGCATCT CCTCCCCTAG CCTTGTGGTA
    GAGTGCGGGG GCCAGACAGT GCAGTCCTGT GTCATCAAGA ACCTCCGGAA GAACCCCAAC
    TTCGACATCT GTACCCTTTT CATGGAAGTG ATGCTGCCCA AGGAGGAGGT CTACTGTCCC
    TCCATCGTGA TCAAGGTCAT TGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG
    TGTACCATCC GTTCCCTGGA GAGCTTCATG TGTGACCTGT ACTCCGAGGA GAATCCGTCC
    CCACAGGGCG GCCCAGATGA TGTGAGTTTA CTCAGTCCTG GGGAAGACGT GCTCATTGAC
    ATCGATGACA AGGAGCCCCT CATCCCCATG CAGGAGGAAG AGTTCATCGA CTGGTGGAGC
    AAATTCTTTG CCTCCACAGG GGAGAGGGAA AAGTGCGGCT CATACTTGGA GAAGGGCTTT
    GACACTCTAA AGGTCTATGA CACACAACTG GAGAACGTGG AGGCCTTTGA GGGCCTGTCT
    GACTTTTGTA ACACCTTCAA GCTCTACCGG GGCAAGACTC AGGAGGAGAC AGAGGACCCA
    TCTGTTATTG GGGAGTTTAA GGGCCTCTTC AAAATCTATC CCCTCCCAGA AGACCCGGCT
    GTTCCCATGC CCCCGAGACA ATTCCACCAG CTGGCTGCCC AAGGGCCTCA GGAGTGCCTG
    GTCCGCATCT ATATCATCCG AGCATTTGGC CTTCAGCCCA AGGACCCCAA TGGGAAGTGT
    GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATT
    CCCTGTACTC TGGAGCCTGT GTTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG
    GAGAAGGACC TGAAGATCAC ACTCTATGAC TATGACCTCC TCTCCAAAGA TGAAAAGATT
    GGGGAGACAG TCATTGACCT GGAGGATAGG CTGCTGTCCA AGTTTGGGGC TCGCTGCGGA
    CTCCCACAGA CGTACTGTGT CTCTGGACCC AACCAGTGGA GGGACCAGCT CCGCCCCTCC
    CAGCTTCTCC ACCTCTTCTG CCAGCAGCGC AGACTCAAGG CTCCTGTGTA CCTCACGGAC
    CACGTGGTGT TCCAGGATAA AGAATACAGC ATTGAAGAAA TAGAGGCTGG CCGAGTCCCG
    AACCCACACC TGGGCCCAGT GGAAGAGCGT CTGGCCTTGC ATGTCCTCCA GCAGCAGGGC
    CTGGTCCCTG AGCATGTGGA GTCCCGGCCT CTCTACAGCC CCCTGCAGCC AGACATTGAA
    CAGGGGAAGC TGCAGATGTG GATCGACCTA TTTCCCAAGG CCCTGGGGCG TCCCGGACCT
    CCCTTCAACA TCACCCCGCG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT CATCTGGAAC
    ACAAAGGACG TGATCCTGGA CGACCTCAGC ATCACAGGGG AGAAGATGAG TGATATTTAT
    GTGAAGGGTT GGATGGTTGG CTTTGAAGAG CACAAGCAAA AGACAGATGT GCATTACCGG
    TCCCTGGGAG GTGAAGGCAA CTTTAACTGG AGGTTCATTT TCCCCTTTGA CTACCTGCCA
    GCCGAGCAAG TCTGCACCGT CTCTAAGAAG GATGCTTTCT GGAGGCTGGA CAAGACTGAG
    AGCAAAATCC CAGCACGAGT GGTATTTCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAC
    GACTTTCTGG GCTCCTTGCA GTTGGATCTC AACCACATGC CCAAGCCTGC CAAGACAGCT
    GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACTTTCCACC CGGAGTGGTT TGTGTCCCTT
    TTTGAACAGA AAACAGTTAA GGGCTGGTGG CCCTGCGTAG CAGAAGAGGG CGAGAAGAAG
    ATCCTGGCAG GCAAGCTGGA AATGACCTTG GAGATCGTAG CAGAGAGTGA GCATGAGGAG
    CGGCCTGCTG GCCAGGGCCG GGATGAACCC AACATGAACC CCAAGCTCGA GGACCCAAGG
    CGCCCTGACA CCTCCTTCCT CTGGTTCACC TCTCCATACA AGACTATGAA GTTCATCCTG
    TGGCGGCGGT TCCGATGTGC CATTATCCTC TTCATCATTC TCTTCATCCT GCTACTATTC
    TTAGGCATCT TCATCTATGC TTTCCCGAAC TACGCTGCCA TGAAGCTGGT GAAGCCCTTC
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003432281.1
    CDS51..6356
    Translation

    Target ORF information:

    RefSeq Version XM_003432233.3
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris dysferlin (DYSF), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003432233.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG
    CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC
    GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG
    AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT
    CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG
    ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT
    GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT
    TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC
    GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT
    ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT
    GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC
    GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC
    AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC
    AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC
    ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG
    GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT
    GCCCCTGGAG GAGAAATAGA AGTGGATGAC CATCTGGGCT TCCTTCCCAC CTTCGGGCCC
    TGCTTCGTCA ATCTCTATGG CAGCCCCAGG GAATTCACTG GCTTCCCGGA CCCCTACGCG
    GAACTCAACA TGGGCAAGGG GGAAGGCGTG GCCTACCGAG GTCGGCTTCT GCTCTCCCTG
    GAGACCAAGC TGGTGGAGCA CAGCGGCGAG CAAAGGGTGG AGGACCTCCC TGCTGATGAC
    ATCCTTCGGG TGGAGAAATA CCTCCGGAGG CGCAAGTACT CTCTCTTTGC CGCCTTCTAC
    TCGGCCACCA TGCTCCAGGA TGTCGACGAT GCCATCCAGT TTGAGGTCAG CATTGGGAAC
    TATGGCAACA AGTTCGACAC GACCTGTCTG CCACTGGCCT CCACCACCCA GTACAGCCGG
    GCAGTCTTTG ATGGGTGCCA CTACTACTAC TTGCCCTGGG GCAATGTGAA GCCTGTGGTG
    GTGCTGTCAT CCTACTGGGA AGACATCAGC CACAGAGTCG AGGCTCAGAA CCAGCTGCTC
    AGGATCGCTG ACCATCTGGA AGCTGGCCTG GAGCAAGTCC ACTTGGCTTT AAAGGCGCAG
    TTCAACACAG AGGATGTGGA CTCCCTGTTG GCCCAGCTGA TGGATGAGCT CATCTCAGAC
    TGCAGCCAGC CCCTGGGCAA TGTCCAAGAG ATACCCTCTG CCACCCACCT GGACCGGCAC
    CTGTACAGGC TGCGCACCCG TCACCTAGGC CAGATTACTG AGGCGGCCCT GGCTCTGAAA
    CTCGGGCATG GCAAGCTCCC TGCCGCCCTG GAGCAGGCTG AAGACTGGCT CCTGCGTCTC
    CGTGCCCTGG CTGATGAGCC CCAGAACAGC CTGCCCGACA TCATCATCTG GATGCTGCAG
    GGAAACAAGC GAGTGGCGTA CCAGCGGGTG CCTGCGCACG AGGTCCTCTT CTCCCGGCGG
    GGCGCCAGCT ACTGTGGCAA GAACTGTGGG AAGCTGCAGA CGATATTTCT GAAATATCCC
    ATGGAGGGGG TGCCCGGAGC CCGGATGCCA GTACAAATAC GGGTGAAACT CTGGTTTGGG
    CTTTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GAAAGCTGTC CGTCTTTGCT
    GAAACTTATG AGAACCAGAC CAAACTGGCC CTGGTTGGGA ACTGGGGCAC AACAGGCCTC
    ACCTACCCCA AGTTTTCTGA TGTCATGGGC AAGATCAAGC TACCCAAGGA TAGCTTCCGC
    CCCTCAGCTG GCTGGACCTG GGCCGGAGAC TGGTTCGTGT GTCCAGAGAA GACCCTACTC
    CATGACATGG ACGCGGGCCA CCTGAGTTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG
    CTCCCTGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CAGATGTGAA TGGGGATAAG
    GTGCTTCCTA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAAGAGTGG
    TCCACGGACC TCAATCGGGC CGTCGATGAG CAAGGCTGGG AGTACAGTAT CACCATCCCC
    CCGGATCGGA AGCCAAAGCA CTGGGTTCCT GCTGAGAAGA TGTATTACAC GCACCGTCGG
    CGGCGCTGGG TCCGCCTGCG CAGAAGGGAC CTCAGCCAGA TGGAGGCATT GAAGAGGCAC
    AGACAGGCAG AGGCAGAGGG TGAGGGCTGG GAGTACGCCT CGCTCTTGGG CTGGAAGTTC
    CACCTGGAGT ACCGCAAGAC TGATGCTTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAA
    CCGCTGGAGA AGACGGGGCC TGCAGCCGTG TTTGCCCTTG AGGGGGCTCT GGGTGGTGTG
    GTAGATGACA GGAGTGAAGA TTCCGTGTCC GTGTCCACCT TGAGCTTTGG AGTGAACAGA
    CCCACGATTT CCTGCATCTT TGATTATGGG AACCGCTACC ACCTACGCTG CTACATGTAC
    CAGGCTCGGG ACCTGCCCTC GATGGACAAG GACTCTTTTT CTGATCCCTA CGCCATCGTC
    TCCTTCCTGC ACCAGAGCCA GAAGACAGTG GTGGCGAAGA ACACTCTGAA CCCCACCTGG
    GACCAGACGC TTATCTTCTA TGAGATTGAG ATCTTTGGTG AGCCAGCCAG TATCGCTGAG
    CAGCCGCCCA GCATTGTGGT GGAGCTGTAT GACCACGACA CCTACGGTGC AGATGAGTTT
    ATGGGACGCT GCATTTGTCA GCCCAGCCTG GAGCGGACAC CCCGGCTGGC CTGGTTCCCA
    CTGATTAGGG GCAGCCAGTC GGCGGGGGAA CTGCTGGCCT CTTTTGAGCT CATCCAGAGA
    GAAAAGCCGG CCATTCACCA TATTCCTGGT TTTGAGGTAC AGGATATGTC AGGAATCCTG
    GAAGAGTCAG AGGACATAGA CCTGCCCTAC CCGCCACCTC AGAGGGAGGC CAACATCTTC
    ATGGTCCCCC AGAACATCAA GCCTGTTCTC CAGCGTACTG CCATAGAGAT CCTGGCGTGG
    GGCCTGCGGA ACATGAAGAG CTACCAGCTA GCCAGCATCT CCTCCCCTAG CCTTGTGGTA
    GAGTGCGGGG GCCAGACAGT GCAGTCCTGT GTCATCAAGA ACCTCCGGAA GAACCCCAAC
    TTCGACATCT GTACCCTTTT CATGGAAGTG ATGCTGCCCA AGGAGGAGGT CTACTGTCCC
    TCCATCGTGA TCAAGGTCAT TGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG
    TGTACCATCC GTTCCCTGGA GAGCTTCATG TGTGACCTGT ACTCCGAGGA GAATCCGTCC
    CCACAGGGCG GCCCAGATGA TGTGAGTTTA CTCAGTCCTG GGGAAGACGT GCTCATTGAC
    ATCGATGACA AGGAGCCCCT CATCCCCATG CAGGAGGAAG AGTTCATCGA CTGGTGGAGC
    AAATTCTTTG CCTCCACAGG GGAGAGGGAA AAGTGCGGCT CATACTTGGA GAAGGGCTTT
    GACACTCTAA AGGTCTATGA CACACAACTG GAGAACGTGG AGGCCTTTGA GGGCCTGTCT
    GACTTTTGTA ACACCTTCAA GCTCTACCGG GGCAAGACTC AGGAGGAGAC AGAGGACCCA
    TCTGTTATTG GGGAGTTTAA GGGCCTCTTC AAAATCTATC CCCTCCCAGA AGACCCGGCT
    GTTCCCATGC CCCCGAGACA ATTCCACCAG CTGGCTGCCC AAGGGCCTCA GGAGTGCCTG
    GTCCGCATCT ATATCATCCG AGCATTTGGC CTTCAGCCCA AGGACCCCAA TGGGAAGTGT
    GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATT
    CCCTGTACTC TGGAGCCTGT GTTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG
    GAGAAGGACC TGAAGATCAC ACTCTATGAC TATGACCTCC TCTCCAAAGA TGAAAAGATT
    GGGGAGACAG TCATTGACCT GGAGGATAGG CTGCTGTCCA AGTTTGGGGC TCGCTGCGGA
    CTCCCACAGA CGTACTGTGT CTCTGGACCC AACCAGTGGA GGGACCAGCT CCGCCCCTCC
    CAGCTTCTCC ACCTCTTCTG CCAGCAGCGC AGACTCAAGG CTCCTGTGTA CCTCACGGAC
    CACGTGGTGT TCCAGGATAA AGAATACAGC ATTGAAGAAA TAGAGGCTGG CCGAGTCCCG
    AACCCACACC TGGGCCCAGT GGAAGAGCGT CTGGCCTTGC ATGTCCTCCA GCAGCAGGGC
    CTGGTCCCTG AGCATGTGGA GTCCCGGCCT CTCTACAGCC CCCTGCAGCC AGACATTGAA
    CAGGGGAAGC TGCAGATGTG GATCGACCTA TTTCCCAAGG CCCTGGGGCG TCCCGGACCT
    CCCTTCAACA TCACCCCGCG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT CATCTGGAAC
    ACAAAGGACG TGATCCTGGA CGACCTCAGC ATCACAGGGG AGAAGATGAG TGATATTTAT
    GTGAAGGGTT GGATGGTTGG CTTTGAAGAG CACAAGCAAA AGACAGATGT GCATTACCGG
    TCCCTGGGAG GTGAAGGCAA CTTTAACTGG AGGTTCATTT TCCCCTTTGA CTACCTGCCA
    GCCGAGCAAG TCTGCACCGT CTCTAAGAAG GATGCTTTCT GGAGGCTGGA CAAGACTGAG
    AGCAAAATCC CAGCACGAGT GGTATTTCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAC
    GACTTTCTGG GCTCCTTGCA GTTGGATCTC AACCACATGC CCAAGCCTGC CAAGACAGCT
    GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACTTTCCACC CGGAGTGGTT TGTGTCCCTT
    TTTGAACAGA AAACAGTTAA GGGCTGGTGG CCCTGCGTAG CAGAAGAGGG CGAGAAGAAG
    ATCCTGGCAG GCAAGCTGGA AATGACCTTG GAGATCGTAG CAGAGAGTGA GCATGAGGAG
    CGGCCTGCTG GCCAGGGCCG GGATGAACCC AACATGAACC CCAAGCTCGA GGACCCAAGG
    CGCCCTGACA CCTCCTTCCT CTGGTTCACC TCTCCATACA AGACTATGAA GTTCATCCTG
    TGGCGGCGGT TCCGATGTGC CATTATCCTC TTCATCATTC TCTTCATCCT GCTACTATTC
    TTAGGCATCT TCATCTATGC TTTCCCGAAC TACGCTGCCA TGAAGCTGGT GAAGCCCTTC
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe19212
    Clone ID Related Accession (Same CDS sequence) XM_003432233.3 , XM_003432233.4
    Accession Version XM_003432233.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6306bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product dysferlin isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003432233.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG
    CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC
    GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG
    AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT
    CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG
    ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT
    GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT
    TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC
    GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT
    ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT
    GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC
    GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC
    AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC
    AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC
    ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG
    GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT
    GCCCCTGGAG GAGAAATAGA AGTGGATGAC CATCTGGGCT TCCTTCCCAC CTTCGGGCCC
    TGCTTCGTCA ATCTCTATGG CAGCCCCAGG GAATTCACTG GCTTCCCGGA CCCCTACGCG
    GAACTCAACA TGGGCAAGGG GGAAGGCGTG GCCTACCGAG GTCGGCTTCT GCTCTCCCTG
    GAGACCAAGC TGGTGGAGCA CAGCGGCGAG CAAAGGGTGG AGGACCTCCC TGCTGATGAC
    ATCCTTCGGG TGGAGAAATA CCTCCGGAGG CGCAAGTACT CTCTCTTTGC CGCCTTCTAC
    TCGGCCACCA TGCTCCAGGA TGTCGACGAT GCCATCCAGT TTGAGGTCAG CATTGGGAAC
    TATGGCAACA AGTTCGACAC GACCTGTCTG CCACTGGCCT CCACCACCCA GTACAGCCGG
    GCAGTCTTTG ATGGGTGCCA CTACTACTAC TTGCCCTGGG GCAATGTGAA GCCTGTGGTG
    GTGCTGTCAT CCTACTGGGA AGACATCAGC CACAGAGTCG AGGCTCAGAA CCAGCTGCTC
    AGGATCGCTG ACCATCTGGA AGCTGGCCTG GAGCAAGTCC ACTTGGCTTT AAAGGCGCAG
    TTCAACACAG AGGATGTGGA CTCCCTGTTG GCCCAGCTGA TGGATGAGCT CATCTCAGAC
    TGCAGCCAGC CCCTGGGCAA TGTCCAAGAG ATACCCTCTG CCACCCACCT GGACCGGCAC
    CTGTACAGGC TGCGCACCCG TCACCTAGGC CAGATTACTG AGGCGGCCCT GGCTCTGAAA
    CTCGGGCATG GCAAGCTCCC TGCCGCCCTG GAGCAGGCTG AAGACTGGCT CCTGCGTCTC
    CGTGCCCTGG CTGATGAGCC CCAGAACAGC CTGCCCGACA TCATCATCTG GATGCTGCAG
    GGAAACAAGC GAGTGGCGTA CCAGCGGGTG CCTGCGCACG AGGTCCTCTT CTCCCGGCGG
    GGCGCCAGCT ACTGTGGCAA GAACTGTGGG AAGCTGCAGA CGATATTTCT GAAATATCCC
    ATGGAGGGGG TGCCCGGAGC CCGGATGCCA GTACAAATAC GGGTGAAACT CTGGTTTGGG
    CTTTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GAAAGCTGTC CGTCTTTGCT
    GAAACTTATG AGAACCAGAC CAAACTGGCC CTGGTTGGGA ACTGGGGCAC AACAGGCCTC
    ACCTACCCCA AGTTTTCTGA TGTCATGGGC AAGATCAAGC TACCCAAGGA TAGCTTCCGC
    CCCTCAGCTG GCTGGACCTG GGCCGGAGAC TGGTTCGTGT GTCCAGAGAA GACCCTACTC
    CATGACATGG ACGCGGGCCA CCTGAGTTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG
    CTCCCTGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CAGATGTGAA TGGGGATAAG
    GTGCTTCCTA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAAGAGTGG
    TCCACGGACC TCAATCGGGC CGTCGATGAG CAAGGCTGGG AGTACAGTAT CACCATCCCC
    CCGGATCGGA AGCCAAAGCA CTGGGTTCCT GCTGAGAAGA TGTATTACAC GCACCGTCGG
    CGGCGCTGGG TCCGCCTGCG CAGAAGGGAC CTCAGCCAGA TGGAGGCATT GAAGAGGCAC
    AGACAGGCAG AGGCAGAGGG TGAGGGCTGG GAGTACGCCT CGCTCTTGGG CTGGAAGTTC
    CACCTGGAGT ACCGCAAGAC TGATGCTTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAA
    CCGCTGGAGA AGACGGGGCC TGCAGCCGTG TTTGCCCTTG AGGGGGCTCT GGGTGGTGTG
    GTAGATGACA GGAGTGAAGA TTCCGTGTCC GTGTCCACCT TGAGCTTTGG AGTGAACAGA
    CCCACGATTT CCTGCATCTT TGATTATGGG AACCGCTACC ACCTACGCTG CTACATGTAC
    CAGGCTCGGG ACCTGCCCTC GATGGACAAG GACTCTTTTT CTGATCCCTA CGCCATCGTC
    TCCTTCCTGC ACCAGAGCCA GAAGACAGTG GTGGCGAAGA ACACTCTGAA CCCCACCTGG
    GACCAGACGC TTATCTTCTA TGAGATTGAG ATCTTTGGTG AGCCAGCCAG TATCGCTGAG
    CAGCCGCCCA GCATTGTGGT GGAGCTGTAT GACCACGACA CCTACGGTGC AGATGAGTTT
    ATGGGACGCT GCATTTGTCA GCCCAGCCTG GAGCGGACAC CCCGGCTGGC CTGGTTCCCA
    CTGATTAGGG GCAGCCAGTC GGCGGGGGAA CTGCTGGCCT CTTTTGAGCT CATCCAGAGA
    GAAAAGCCGG CCATTCACCA TATTCCTGGT TTTGAGGTAC AGGATATGTC AGGAATCCTG
    GAAGAGTCAG AGGACATAGA CCTGCCCTAC CCGCCACCTC AGAGGGAGGC CAACATCTTC
    ATGGTCCCCC AGAACATCAA GCCTGTTCTC CAGCGTACTG CCATAGAGAT CCTGGCGTGG
    GGCCTGCGGA ACATGAAGAG CTACCAGCTA GCCAGCATCT CCTCCCCTAG CCTTGTGGTA
    GAGTGCGGGG GCCAGACAGT GCAGTCCTGT GTCATCAAGA ACCTCCGGAA GAACCCCAAC
    TTCGACATCT GTACCCTTTT CATGGAAGTG ATGCTGCCCA AGGAGGAGGT CTACTGTCCC
    TCCATCGTGA TCAAGGTCAT TGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG
    TGTACCATCC GTTCCCTGGA GAGCTTCATG TGTGACCTGT ACTCCGAGGA GAATCCGTCC
    CCACAGGGCG GCCCAGATGA TGTGAGTTTA CTCAGTCCTG GGGAAGACGT GCTCATTGAC
    ATCGATGACA AGGAGCCCCT CATCCCCATG CAGGAGGAAG AGTTCATCGA CTGGTGGAGC
    AAATTCTTTG CCTCCACAGG GGAGAGGGAA AAGTGCGGCT CATACTTGGA GAAGGGCTTT
    GACACTCTAA AGGTCTATGA CACACAACTG GAGAACGTGG AGGCCTTTGA GGGCCTGTCT
    GACTTTTGTA ACACCTTCAA GCTCTACCGG GGCAAGACTC AGGAGGAGAC AGAGGACCCA
    TCTGTTATTG GGGAGTTTAA GGGCCTCTTC AAAATCTATC CCCTCCCAGA AGACCCGGCT
    GTTCCCATGC CCCCGAGACA ATTCCACCAG CTGGCTGCCC AAGGGCCTCA GGAGTGCCTG
    GTCCGCATCT ATATCATCCG AGCATTTGGC CTTCAGCCCA AGGACCCCAA TGGGAAGTGT
    GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATT
    CCCTGTACTC TGGAGCCTGT GTTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG
    GAGAAGGACC TGAAGATCAC ACTCTATGAC TATGACCTCC TCTCCAAAGA TGAAAAGATT
    GGGGAGACAG TCATTGACCT GGAGGATAGG CTGCTGTCCA AGTTTGGGGC TCGCTGCGGA
    CTCCCACAGA CGTACTGTGT CTCTGGACCC AACCAGTGGA GGGACCAGCT CCGCCCCTCC
    CAGCTTCTCC ACCTCTTCTG CCAGCAGCGC AGACTCAAGG CTCCTGTGTA CCTCACGGAC
    CACGTGGTGT TCCAGGATAA AGAATACAGC ATTGAAGAAA TAGAGGCTGG CCGAGTCCCG
    AACCCACACC TGGGCCCAGT GGAAGAGCGT CTGGCCTTGC ATGTCCTCCA GCAGCAGGGC
    CTGGTCCCTG AGCATGTGGA GTCCCGGCCT CTCTACAGCC CCCTGCAGCC AGACATTGAA
    CAGGGGAAGC TGCAGATGTG GATCGACCTA TTTCCCAAGG CCCTGGGGCG TCCCGGACCT
    CCCTTCAACA TCACCCCGCG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT CATCTGGAAC
    ACAAAGGACG TGATCCTGGA CGACCTCAGC ATCACAGGGG AGAAGATGAG TGATATTTAT
    GTGAAGGGTT GGATGGTTGG CTTTGAAGAG CACAAGCAAA AGACAGATGT GCATTACCGG
    TCCCTGGGAG GTGAAGGCAA CTTTAACTGG AGGTTCATTT TCCCCTTTGA CTACCTGCCA
    GCCGAGCAAG TCTGCACCGT CTCTAAGAAG GATGCTTTCT GGAGGCTGGA CAAGACTGAG
    AGCAAAATCC CAGCACGAGT GGTATTTCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAC
    GACTTTCTGG GCTCCTTGCA GTTGGATCTC AACCACATGC CCAAGCCTGC CAAGACAGCT
    GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACTTTCCACC CGGAGTGGTT TGTGTCCCTT
    TTTGAACAGA AAACAGTTAA GGGCTGGTGG CCCTGCGTAG CAGAAGAGGG CGAGAAGAAG
    ATCCTGGCAG GCAAGCTGGA AATGACCTTG GAGATCGTAG CAGAGAGTGA GCATGAGGAG
    CGGCCTGCTG GCCAGGGCCG GGATGAACCC AACATGAACC CCAAGCTCGA GGACCCAAGG
    CGCCCTGACA CCTCCTTCCT CTGGTTCACC TCTCCATACA AGACTATGAA GTTCATCCTG
    TGGCGGCGGT TCCGATGTGC CATTATCCTC TTCATCATTC TCTTCATCCT GCTACTATTC
    TTAGGCATCT TCATCTATGC TTTCCCGAAC TACGCTGCCA TGAAGCTGGT GAAGCCCTTC
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003432281.1
    CDS23..6328
    Translation

    Target ORF information:

    RefSeq Version XM_003432233.4
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris dysferlin (DYSF), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003432233.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT
    GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG
    CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC
    GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG
    AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT
    CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG
    ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT
    GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT
    TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC
    GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT
    ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT
    GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC
    GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC
    AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC
    AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT
    GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG
    AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC
    ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG
    GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT
    GCCCCTGGAG GAGAAATAGA AGTGGATGAC CATCTGGGCT TCCTTCCCAC CTTCGGGCCC
    TGCTTCGTCA ATCTCTATGG CAGCCCCAGG GAATTCACTG GCTTCCCGGA CCCCTACGCG
    GAACTCAACA TGGGCAAGGG GGAAGGCGTG GCCTACCGAG GTCGGCTTCT GCTCTCCCTG
    GAGACCAAGC TGGTGGAGCA CAGCGGCGAG CAAAGGGTGG AGGACCTCCC TGCTGATGAC
    ATCCTTCGGG TGGAGAAATA CCTCCGGAGG CGCAAGTACT CTCTCTTTGC CGCCTTCTAC
    TCGGCCACCA TGCTCCAGGA TGTCGACGAT GCCATCCAGT TTGAGGTCAG CATTGGGAAC
    TATGGCAACA AGTTCGACAC GACCTGTCTG CCACTGGCCT CCACCACCCA GTACAGCCGG
    GCAGTCTTTG ATGGGTGCCA CTACTACTAC TTGCCCTGGG GCAATGTGAA GCCTGTGGTG
    GTGCTGTCAT CCTACTGGGA AGACATCAGC CACAGAGTCG AGGCTCAGAA CCAGCTGCTC
    AGGATCGCTG ACCATCTGGA AGCTGGCCTG GAGCAAGTCC ACTTGGCTTT AAAGGCGCAG
    TTCAACACAG AGGATGTGGA CTCCCTGTTG GCCCAGCTGA TGGATGAGCT CATCTCAGAC
    TGCAGCCAGC CCCTGGGCAA TGTCCAAGAG ATACCCTCTG CCACCCACCT GGACCGGCAC
    CTGTACAGGC TGCGCACCCG TCACCTAGGC CAGATTACTG AGGCGGCCCT GGCTCTGAAA
    CTCGGGCATG GCAAGCTCCC TGCCGCCCTG GAGCAGGCTG AAGACTGGCT CCTGCGTCTC
    CGTGCCCTGG CTGATGAGCC CCAGAACAGC CTGCCCGACA TCATCATCTG GATGCTGCAG
    GGAAACAAGC GAGTGGCGTA CCAGCGGGTG CCTGCGCACG AGGTCCTCTT CTCCCGGCGG
    GGCGCCAGCT ACTGTGGCAA GAACTGTGGG AAGCTGCAGA CGATATTTCT GAAATATCCC
    ATGGAGGGGG TGCCCGGAGC CCGGATGCCA GTACAAATAC GGGTGAAACT CTGGTTTGGG
    CTTTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GAAAGCTGTC CGTCTTTGCT
    GAAACTTATG AGAACCAGAC CAAACTGGCC CTGGTTGGGA ACTGGGGCAC AACAGGCCTC
    ACCTACCCCA AGTTTTCTGA TGTCATGGGC AAGATCAAGC TACCCAAGGA TAGCTTCCGC
    CCCTCAGCTG GCTGGACCTG GGCCGGAGAC TGGTTCGTGT GTCCAGAGAA GACCCTACTC
    CATGACATGG ACGCGGGCCA CCTGAGTTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG
    CTCCCTGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CAGATGTGAA TGGGGATAAG
    GTGCTTCCTA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAAGAGTGG
    TCCACGGACC TCAATCGGGC CGTCGATGAG CAAGGCTGGG AGTACAGTAT CACCATCCCC
    CCGGATCGGA AGCCAAAGCA CTGGGTTCCT GCTGAGAAGA TGTATTACAC GCACCGTCGG
    CGGCGCTGGG TCCGCCTGCG CAGAAGGGAC CTCAGCCAGA TGGAGGCATT GAAGAGGCAC
    AGACAGGCAG AGGCAGAGGG TGAGGGCTGG GAGTACGCCT CGCTCTTGGG CTGGAAGTTC
    CACCTGGAGT ACCGCAAGAC TGATGCTTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAA
    CCGCTGGAGA AGACGGGGCC TGCAGCCGTG TTTGCCCTTG AGGGGGCTCT GGGTGGTGTG
    GTAGATGACA GGAGTGAAGA TTCCGTGTCC GTGTCCACCT TGAGCTTTGG AGTGAACAGA
    CCCACGATTT CCTGCATCTT TGATTATGGG AACCGCTACC ACCTACGCTG CTACATGTAC
    CAGGCTCGGG ACCTGCCCTC GATGGACAAG GACTCTTTTT CTGATCCCTA CGCCATCGTC
    TCCTTCCTGC ACCAGAGCCA GAAGACAGTG GTGGCGAAGA ACACTCTGAA CCCCACCTGG
    GACCAGACGC TTATCTTCTA TGAGATTGAG ATCTTTGGTG AGCCAGCCAG TATCGCTGAG
    CAGCCGCCCA GCATTGTGGT GGAGCTGTAT GACCACGACA CCTACGGTGC AGATGAGTTT
    ATGGGACGCT GCATTTGTCA GCCCAGCCTG GAGCGGACAC CCCGGCTGGC CTGGTTCCCA
    CTGATTAGGG GCAGCCAGTC GGCGGGGGAA CTGCTGGCCT CTTTTGAGCT CATCCAGAGA
    GAAAAGCCGG CCATTCACCA TATTCCTGGT TTTGAGGTAC AGGATATGTC AGGAATCCTG
    GAAGAGTCAG AGGACATAGA CCTGCCCTAC CCGCCACCTC AGAGGGAGGC CAACATCTTC
    ATGGTCCCCC AGAACATCAA GCCTGTTCTC CAGCGTACTG CCATAGAGAT CCTGGCGTGG
    GGCCTGCGGA ACATGAAGAG CTACCAGCTA GCCAGCATCT CCTCCCCTAG CCTTGTGGTA
    GAGTGCGGGG GCCAGACAGT GCAGTCCTGT GTCATCAAGA ACCTCCGGAA GAACCCCAAC
    TTCGACATCT GTACCCTTTT CATGGAAGTG ATGCTGCCCA AGGAGGAGGT CTACTGTCCC
    TCCATCGTGA TCAAGGTCAT TGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG
    TGTACCATCC GTTCCCTGGA GAGCTTCATG TGTGACCTGT ACTCCGAGGA GAATCCGTCC
    CCACAGGGCG GCCCAGATGA TGTGAGTTTA CTCAGTCCTG GGGAAGACGT GCTCATTGAC
    ATCGATGACA AGGAGCCCCT CATCCCCATG CAGGAGGAAG AGTTCATCGA CTGGTGGAGC
    AAATTCTTTG CCTCCACAGG GGAGAGGGAA AAGTGCGGCT CATACTTGGA GAAGGGCTTT
    GACACTCTAA AGGTCTATGA CACACAACTG GAGAACGTGG AGGCCTTTGA GGGCCTGTCT
    GACTTTTGTA ACACCTTCAA GCTCTACCGG GGCAAGACTC AGGAGGAGAC AGAGGACCCA
    TCTGTTATTG GGGAGTTTAA GGGCCTCTTC AAAATCTATC CCCTCCCAGA AGACCCGGCT
    GTTCCCATGC CCCCGAGACA ATTCCACCAG CTGGCTGCCC AAGGGCCTCA GGAGTGCCTG
    GTCCGCATCT ATATCATCCG AGCATTTGGC CTTCAGCCCA AGGACCCCAA TGGGAAGTGT
    GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATT
    CCCTGTACTC TGGAGCCTGT GTTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG
    GAGAAGGACC TGAAGATCAC ACTCTATGAC TATGACCTCC TCTCCAAAGA TGAAAAGATT
    GGGGAGACAG TCATTGACCT GGAGGATAGG CTGCTGTCCA AGTTTGGGGC TCGCTGCGGA
    CTCCCACAGA CGTACTGTGT CTCTGGACCC AACCAGTGGA GGGACCAGCT CCGCCCCTCC
    CAGCTTCTCC ACCTCTTCTG CCAGCAGCGC AGACTCAAGG CTCCTGTGTA CCTCACGGAC
    CACGTGGTGT TCCAGGATAA AGAATACAGC ATTGAAGAAA TAGAGGCTGG CCGAGTCCCG
    AACCCACACC TGGGCCCAGT GGAAGAGCGT CTGGCCTTGC ATGTCCTCCA GCAGCAGGGC
    CTGGTCCCTG AGCATGTGGA GTCCCGGCCT CTCTACAGCC CCCTGCAGCC AGACATTGAA
    CAGGGGAAGC TGCAGATGTG GATCGACCTA TTTCCCAAGG CCCTGGGGCG TCCCGGACCT
    CCCTTCAACA TCACCCCGCG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT CATCTGGAAC
    ACAAAGGACG TGATCCTGGA CGACCTCAGC ATCACAGGGG AGAAGATGAG TGATATTTAT
    GTGAAGGGTT GGATGGTTGG CTTTGAAGAG CACAAGCAAA AGACAGATGT GCATTACCGG
    TCCCTGGGAG GTGAAGGCAA CTTTAACTGG AGGTTCATTT TCCCCTTTGA CTACCTGCCA
    GCCGAGCAAG TCTGCACCGT CTCTAAGAAG GATGCTTTCT GGAGGCTGGA CAAGACTGAG
    AGCAAAATCC CAGCACGAGT GGTATTTCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAC
    GACTTTCTGG GCTCCTTGCA GTTGGATCTC AACCACATGC CCAAGCCTGC CAAGACAGCT
    GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACTTTCCACC CGGAGTGGTT TGTGTCCCTT
    TTTGAACAGA AAACAGTTAA GGGCTGGTGG CCCTGCGTAG CAGAAGAGGG CGAGAAGAAG
    ATCCTGGCAG GCAAGCTGGA AATGACCTTG GAGATCGTAG CAGAGAGTGA GCATGAGGAG
    CGGCCTGCTG GCCAGGGCCG GGATGAACCC AACATGAACC CCAAGCTCGA GGACCCAAGG
    CGCCCTGACA CCTCCTTCCT CTGGTTCACC TCTCCATACA AGACTATGAA GTTCATCCTG
    TGGCGGCGGT TCCGATGTGC CATTATCCTC TTCATCATTC TCTTCATCCT GCTACTATTC
    TTAGGCATCT TCATCTATGC TTTCCCGAAC TACGCTGCCA TGAAGCTGGT GAAGCCCTTC
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe19213
    Clone ID Related Accession (Same CDS sequence) XM_003432228.3 , XM_003432228.4
    Accession Version XM_003432228.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6303bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product dysferlin isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003432228.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC
    CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC
    TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA
    CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGGTG GGGGACAGAG CCGGGCTGAG
    ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GATACAAGAT ACTCTGGAAA GAAGTGGCTG
    GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC TGGAGACGAG
    GGAGAGCCAT TACTGGATCA GAGTGGCGTC CCAGGCCCTG GGGCTCCCAC CACCCCGAGG
    AAGCCACCTT CCCATCTGCC CCCCTACCAC CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC
    GCAGCCAGAA AGCTGCTGTC CGACAAACCG CAGGACTTCC AGATCAGAGT GCAAGTGATC
    GAGGGCCGCC AGCTGCCAGG GGTGAATATC AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG
    CAGACCAAGA GGACACGGAT CCACAAGGGA AACAGCCCAC TATTTAACGA GACTCTTTTC
    TTCAATGTGT TTGACTCTCC TTCGGAGCTG TTTGATGAGC CCATCTTTAT CACGGTCGTA
    GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC
    ATCTACAGAG AGCCCCGACA TGCTTATCTG AGGAAGTGGC TGCTACTCTC CAACCCTGAT
    GACTTCTCTG TTGGGGCCAG AGGTTACCTG AAAGCAAGCC TTTATGTGCT GGGGCCCGGA
    GATGAAGCGC CTCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT TGAAAACAAT
    CTGCTCAGGC CAACTGGCGT GGCCCTGCGA GGAGCCCACT TCTGCCTGAA GATCTTCAGA
    GCGGAGGACT TACCACAAAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC
    TTTGACAGCA ACAAGAAGAA TTTGGTAGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA
    ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACT
    CTGCCTGCCA TGTTTCCCTC CATGTGTGAA AAAATGAAGA TTCGCGTCAT GGACTGGGAC
    CGCCTTACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC
    CCTGGAGGAG AAATAGAAGT GGATGACCAT CTGGGCTTCC TTCCCACCTT CGGGCCCTGC
    TTCGTCAATC TCTATGGCAG CCCCAGGGAA TTCACTGGCT TCCCGGACCC CTACGCGGAA
    CTCAACATGG GCAAGGGGGA AGGCGTGGCC TACCGAGGTC GGCTTCTGCT CTCCCTGGAG
    ACCAAGCTGG TGGAGCACAG CGGCGAGCAA AGGGTGGAGG ACCTCCCTGC TGATGACATC
    CTTCGGGTGG AGAAATACCT CCGGAGGCGC AAGTACTCTC TCTTTGCCGC CTTCTACTCG
    GCCACCATGC TCCAGGATGT CGACGATGCC ATCCAGTTTG AGGTCAGCAT TGGGAACTAT
    GGCAACAAGT TCGACACGAC CTGTCTGCCA CTGGCCTCCA CCACCCAGTA CAGCCGGGCA
    GTCTTTGATG GGTGCCACTA CTACTACTTG CCCTGGGGCA ATGTGAAGCC TGTGGTGGTG
    CTGTCATCCT ACTGGGAAGA CATCAGCCAC AGAGTCGAGG CTCAGAACCA GCTGCTCAGG
    ATCGCTGACC ATCTGGAAGC TGGCCTGGAG CAAGTCCACT TGGCTTTAAA GGCGCAGTTC
    AACACAGAGG ATGTGGACTC CCTGTTGGCC CAGCTGATGG ATGAGCTCAT CTCAGACTGC
    AGCCAGCCCC TGGGCAATGT CCAAGAGATA CCCTCTGCCA CCCACCTGGA CCGGCACCTG
    TACAGGCTGC GCACCCGTCA CCTAGGCCAG ATTACTGAGG CGGCCCTGGC TCTGAAACTC
    GGGCATGGCA AGCTCCCTGC CGCCCTGGAG CAGGCTGAAG ACTGGCTCCT GCGTCTCCGT
    GCCCTGGCTG ATGAGCCCCA GAACAGCCTG CCCGACATCA TCATCTGGAT GCTGCAGGGA
    AACAAGCGAG TGGCGTACCA GCGGGTGCCT GCGCACGAGG TCCTCTTCTC CCGGCGGGGC
    GCCAGCTACT GTGGCAAGAA CTGTGGGAAG CTGCAGACGA TATTTCTGAA ATATCCCATG
    GAGGGGGTGC CCGGAGCCCG GATGCCAGTA CAAATACGGG TGAAACTCTG GTTTGGGCTT
    TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGAA AGCTGTCCGT CTTTGCTGAA
    ACTTATGAGA ACCAGACCAA ACTGGCCCTG GTTGGGAACT GGGGCACAAC AGGCCTCACC
    TACCCCAAGT TTTCTGATGT CATGGGCAAG ATCAAGCTAC CCAAGGATAG CTTCCGCCCC
    TCAGCTGGCT GGACCTGGGC CGGAGACTGG TTCGTGTGTC CAGAGAAGAC CCTACTCCAT
    GACATGGACG CGGGCCACCT GAGTTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTC
    CCTGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACAG ATGTGAATGG GGATAAGGTG
    CTTCCTAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA AGAGTGGTCC
    ACGGACCTCA ATCGGGCCGT CGATGAGCAA GGCTGGGAGT ACAGTATCAC CATCCCCCCG
    GATCGGAAGC CAAAGCACTG GGTTCCTGCT GAGAAGATGT ATTACACGCA CCGTCGGCGG
    CGCTGGGTCC GCCTGCGCAG AAGGGACCTC AGCCAGATGG AGGCATTGAA GAGGCACAGA
    CAGGCAGAGG CAGAGGGTGA GGGCTGGGAG TACGCCTCGC TCTTGGGCTG GAAGTTCCAC
    CTGGAGTACC GCAAGACTGA TGCTTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAACCG
    CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT GCCCTTGAGG GGGCTCTGGG TGGTGTGGTA
    GATGACAGGA GTGAAGATTC CGTGTCCGTG TCCACCTTGA GCTTTGGAGT GAACAGACCC
    ACGATTTCCT GCATCTTTGA TTATGGGAAC CGCTACCACC TACGCTGCTA CATGTACCAG
    GCTCGGGACC TGCCCTCGAT GGACAAGGAC TCTTTTTCTG ATCCCTACGC CATCGTCTCC
    TTCCTGCACC AGAGCCAGAA GACAGTGGTG GCGAAGAACA CTCTGAACCC CACCTGGGAC
    CAGACGCTTA TCTTCTATGA GATTGAGATC TTTGGTGAGC CAGCCAGTAT CGCTGAGCAG
    CCGCCCAGCA TTGTGGTGGA GCTGTATGAC CACGACACCT ACGGTGCAGA TGAGTTTATG
    GGACGCTGCA TTTGTCAGCC CAGCCTGGAG CGGACACCCC GGCTGGCCTG GTTCCCACTG
    ATTAGGGGCA GCCAGTCGGC GGGGGAACTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAA
    AAGCCGGCCA TTCACCATAT TCCTGGTTTT GAGGTACAGG ATATGTCAGG AATCCTGGAA
    GAGTCAGAGG ACATAGACCT GCCCTACCCG CCACCTCAGA GGGAGGCCAA CATCTTCATG
    GTCCCCCAGA ACATCAAGCC TGTTCTCCAG CGTACTGCCA TAGAGATCCT GGCGTGGGGC
    CTGCGGAACA TGAAGAGCTA CCAGCTAGCC AGCATCTCCT CCCCTAGCCT TGTGGTAGAG
    TGCGGGGGCC AGACAGTGCA GTCCTGTGTC ATCAAGAACC TCCGGAAGAA CCCCAACTTC
    GACATCTGTA CCCTTTTCAT GGAAGTGATG CTGCCCAAGG AGGAGGTCTA CTGTCCCTCC
    ATCGTGATCA AGGTCATTGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT
    ACCATCCGTT CCCTGGAGAG CTTCATGTGT GACCTGTACT CCGAGGAGAA TCCGTCCCCA
    CAGGGCGGCC CAGATGATGT GAGTTTACTC AGTCCTGGGG AAGACGTGCT CATTGACATC
    GATGACAAGG AGCCCCTCAT CCCCATGCAG GAGGAAGAGT TCATCGACTG GTGGAGCAAA
    TTCTTTGCCT CCACAGGGGA GAGGGAAAAG TGCGGCTCAT ACTTGGAGAA GGGCTTTGAC
    ACTCTAAAGG TCTATGACAC ACAACTGGAG AACGTGGAGG CCTTTGAGGG CCTGTCTGAC
    TTTTGTAACA CCTTCAAGCT CTACCGGGGC AAGACTCAGG AGGAGACAGA GGACCCATCT
    GTTATTGGGG AGTTTAAGGG CCTCTTCAAA ATCTATCCCC TCCCAGAAGA CCCGGCTGTT
    CCCATGCCCC CGAGACAATT CCACCAGCTG GCTGCCCAAG GGCCTCAGGA GTGCCTGGTC
    CGCATCTATA TCATCCGAGC ATTTGGCCTT CAGCCCAAGG ACCCCAATGG GAAGTGTGAT
    CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATTCCC
    TGTACTCTGG AGCCTGTGTT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG
    AAGGACCTGA AGATCACACT CTATGACTAT GACCTCCTCT CCAAAGATGA AAAGATTGGG
    GAGACAGTCA TTGACCTGGA GGATAGGCTG CTGTCCAAGT TTGGGGCTCG CTGCGGACTC
    CCACAGACGT ACTGTGTCTC TGGACCCAAC CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG
    CTTCTCCACC TCTTCTGCCA GCAGCGCAGA CTCAAGGCTC CTGTGTACCT CACGGACCAC
    GTGGTGTTCC AGGATAAAGA ATACAGCATT GAAGAAATAG AGGCTGGCCG AGTCCCGAAC
    CCACACCTGG GCCCAGTGGA AGAGCGTCTG GCCTTGCATG TCCTCCAGCA GCAGGGCCTG
    GTCCCTGAGC ATGTGGAGTC CCGGCCTCTC TACAGCCCCC TGCAGCCAGA CATTGAACAG
    GGGAAGCTGC AGATGTGGAT CGACCTATTT CCCAAGGCCC TGGGGCGTCC CGGACCTCCC
    TTCAACATCA CCCCGCGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATCAT CTGGAACACA
    AAGGACGTGA TCCTGGACGA CCTCAGCATC ACAGGGGAGA AGATGAGTGA TATTTATGTG
    AAGGGTTGGA TGGTTGGCTT TGAAGAGCAC AAGCAAAAGA CAGATGTGCA TTACCGGTCC
    CTGGGAGGTG AAGGCAACTT TAACTGGAGG TTCATTTTCC CCTTTGACTA CCTGCCAGCC
    GAGCAAGTCT GCACCGTCTC TAAGAAGGAT GCTTTCTGGA GGCTGGACAA GACTGAGAGC
    AAAATCCCAG CACGAGTGGT ATTTCAGATC TGGGACAATG ACAAGTTCTC CTTTGACGAC
    TTTCTGGGCT CCTTGCAGTT GGATCTCAAC CACATGCCCA AGCCTGCCAA GACAGCTGAG
    AAGTGCTCCT TGGACCAGCT GGATGACACT TTCCACCCGG AGTGGTTTGT GTCCCTTTTT
    GAACAGAAAA CAGTTAAGGG CTGGTGGCCC TGCGTAGCAG AAGAGGGCGA GAAGAAGATC
    CTGGCAGGCA AGCTGGAAAT GACCTTGGAG ATCGTAGCAG AGAGTGAGCA TGAGGAGCGG
    CCTGCTGGCC AGGGCCGGGA TGAACCCAAC ATGAACCCCA AGCTCGAGGA CCCAAGGCGC
    CCTGACACCT CCTTCCTCTG GTTCACCTCT CCATACAAGA CTATGAAGTT CATCCTGTGG
    CGGCGGTTCC GATGTGCCAT TATCCTCTTC ATCATTCTCT TCATCCTGCT ACTATTCTTA
    GGCATCTTCA TCTATGCTTT CCCGAACTAC GCTGCCATGA AGCTGGTGAA GCCCTTCAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003432276.1
    CDS439..6741
    Translation

    Target ORF information:

    RefSeq Version XM_003432228.3
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris dysferlin (DYSF), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003432228.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC
    CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC
    TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA
    CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGGTG GGGGACAGAG CCGGGCTGAG
    ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GATACAAGAT ACTCTGGAAA GAAGTGGCTG
    GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC TGGAGACGAG
    GGAGAGCCAT TACTGGATCA GAGTGGCGTC CCAGGCCCTG GGGCTCCCAC CACCCCGAGG
    AAGCCACCTT CCCATCTGCC CCCCTACCAC CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC
    GCAGCCAGAA AGCTGCTGTC CGACAAACCG CAGGACTTCC AGATCAGAGT GCAAGTGATC
    GAGGGCCGCC AGCTGCCAGG GGTGAATATC AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG
    CAGACCAAGA GGACACGGAT CCACAAGGGA AACAGCCCAC TATTTAACGA GACTCTTTTC
    TTCAATGTGT TTGACTCTCC TTCGGAGCTG TTTGATGAGC CCATCTTTAT CACGGTCGTA
    GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC
    ATCTACAGAG AGCCCCGACA TGCTTATCTG AGGAAGTGGC TGCTACTCTC CAACCCTGAT
    GACTTCTCTG TTGGGGCCAG AGGTTACCTG AAAGCAAGCC TTTATGTGCT GGGGCCCGGA
    GATGAAGCGC CTCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT TGAAAACAAT
    CTGCTCAGGC CAACTGGCGT GGCCCTGCGA GGAGCCCACT TCTGCCTGAA GATCTTCAGA
    GCGGAGGACT TACCACAAAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC
    TTTGACAGCA ACAAGAAGAA TTTGGTAGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA
    ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACT
    CTGCCTGCCA TGTTTCCCTC CATGTGTGAA AAAATGAAGA TTCGCGTCAT GGACTGGGAC
    CGCCTTACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC
    CCTGGAGGAG AAATAGAAGT GGATGACCAT CTGGGCTTCC TTCCCACCTT CGGGCCCTGC
    TTCGTCAATC TCTATGGCAG CCCCAGGGAA TTCACTGGCT TCCCGGACCC CTACGCGGAA
    CTCAACATGG GCAAGGGGGA AGGCGTGGCC TACCGAGGTC GGCTTCTGCT CTCCCTGGAG
    ACCAAGCTGG TGGAGCACAG CGGCGAGCAA AGGGTGGAGG ACCTCCCTGC TGATGACATC
    CTTCGGGTGG AGAAATACCT CCGGAGGCGC AAGTACTCTC TCTTTGCCGC CTTCTACTCG
    GCCACCATGC TCCAGGATGT CGACGATGCC ATCCAGTTTG AGGTCAGCAT TGGGAACTAT
    GGCAACAAGT TCGACACGAC CTGTCTGCCA CTGGCCTCCA CCACCCAGTA CAGCCGGGCA
    GTCTTTGATG GGTGCCACTA CTACTACTTG CCCTGGGGCA ATGTGAAGCC TGTGGTGGTG
    CTGTCATCCT ACTGGGAAGA CATCAGCCAC AGAGTCGAGG CTCAGAACCA GCTGCTCAGG
    ATCGCTGACC ATCTGGAAGC TGGCCTGGAG CAAGTCCACT TGGCTTTAAA GGCGCAGTTC
    AACACAGAGG ATGTGGACTC CCTGTTGGCC CAGCTGATGG ATGAGCTCAT CTCAGACTGC
    AGCCAGCCCC TGGGCAATGT CCAAGAGATA CCCTCTGCCA CCCACCTGGA CCGGCACCTG
    TACAGGCTGC GCACCCGTCA CCTAGGCCAG ATTACTGAGG CGGCCCTGGC TCTGAAACTC
    GGGCATGGCA AGCTCCCTGC CGCCCTGGAG CAGGCTGAAG ACTGGCTCCT GCGTCTCCGT
    GCCCTGGCTG ATGAGCCCCA GAACAGCCTG CCCGACATCA TCATCTGGAT GCTGCAGGGA
    AACAAGCGAG TGGCGTACCA GCGGGTGCCT GCGCACGAGG TCCTCTTCTC CCGGCGGGGC
    GCCAGCTACT GTGGCAAGAA CTGTGGGAAG CTGCAGACGA TATTTCTGAA ATATCCCATG
    GAGGGGGTGC CCGGAGCCCG GATGCCAGTA CAAATACGGG TGAAACTCTG GTTTGGGCTT
    TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGAA AGCTGTCCGT CTTTGCTGAA
    ACTTATGAGA ACCAGACCAA ACTGGCCCTG GTTGGGAACT GGGGCACAAC AGGCCTCACC
    TACCCCAAGT TTTCTGATGT CATGGGCAAG ATCAAGCTAC CCAAGGATAG CTTCCGCCCC
    TCAGCTGGCT GGACCTGGGC CGGAGACTGG TTCGTGTGTC CAGAGAAGAC CCTACTCCAT
    GACATGGACG CGGGCCACCT GAGTTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTC
    CCTGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACAG ATGTGAATGG GGATAAGGTG
    CTTCCTAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA AGAGTGGTCC
    ACGGACCTCA ATCGGGCCGT CGATGAGCAA GGCTGGGAGT ACAGTATCAC CATCCCCCCG
    GATCGGAAGC CAAAGCACTG GGTTCCTGCT GAGAAGATGT ATTACACGCA CCGTCGGCGG
    CGCTGGGTCC GCCTGCGCAG AAGGGACCTC AGCCAGATGG AGGCATTGAA GAGGCACAGA
    CAGGCAGAGG CAGAGGGTGA GGGCTGGGAG TACGCCTCGC TCTTGGGCTG GAAGTTCCAC
    CTGGAGTACC GCAAGACTGA TGCTTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAACCG
    CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT GCCCTTGAGG GGGCTCTGGG TGGTGTGGTA
    GATGACAGGA GTGAAGATTC CGTGTCCGTG TCCACCTTGA GCTTTGGAGT GAACAGACCC
    ACGATTTCCT GCATCTTTGA TTATGGGAAC CGCTACCACC TACGCTGCTA CATGTACCAG
    GCTCGGGACC TGCCCTCGAT GGACAAGGAC TCTTTTTCTG ATCCCTACGC CATCGTCTCC
    TTCCTGCACC AGAGCCAGAA GACAGTGGTG GCGAAGAACA CTCTGAACCC CACCTGGGAC
    CAGACGCTTA TCTTCTATGA GATTGAGATC TTTGGTGAGC CAGCCAGTAT CGCTGAGCAG
    CCGCCCAGCA TTGTGGTGGA GCTGTATGAC CACGACACCT ACGGTGCAGA TGAGTTTATG
    GGACGCTGCA TTTGTCAGCC CAGCCTGGAG CGGACACCCC GGCTGGCCTG GTTCCCACTG
    ATTAGGGGCA GCCAGTCGGC GGGGGAACTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAA
    AAGCCGGCCA TTCACCATAT TCCTGGTTTT GAGGTACAGG ATATGTCAGG AATCCTGGAA
    GAGTCAGAGG ACATAGACCT GCCCTACCCG CCACCTCAGA GGGAGGCCAA CATCTTCATG
    GTCCCCCAGA ACATCAAGCC TGTTCTCCAG CGTACTGCCA TAGAGATCCT GGCGTGGGGC
    CTGCGGAACA TGAAGAGCTA CCAGCTAGCC AGCATCTCCT CCCCTAGCCT TGTGGTAGAG
    TGCGGGGGCC AGACAGTGCA GTCCTGTGTC ATCAAGAACC TCCGGAAGAA CCCCAACTTC
    GACATCTGTA CCCTTTTCAT GGAAGTGATG CTGCCCAAGG AGGAGGTCTA CTGTCCCTCC
    ATCGTGATCA AGGTCATTGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT
    ACCATCCGTT CCCTGGAGAG CTTCATGTGT GACCTGTACT CCGAGGAGAA TCCGTCCCCA
    CAGGGCGGCC CAGATGATGT GAGTTTACTC AGTCCTGGGG AAGACGTGCT CATTGACATC
    GATGACAAGG AGCCCCTCAT CCCCATGCAG GAGGAAGAGT TCATCGACTG GTGGAGCAAA
    TTCTTTGCCT CCACAGGGGA GAGGGAAAAG TGCGGCTCAT ACTTGGAGAA GGGCTTTGAC
    ACTCTAAAGG TCTATGACAC ACAACTGGAG AACGTGGAGG CCTTTGAGGG CCTGTCTGAC
    TTTTGTAACA CCTTCAAGCT CTACCGGGGC AAGACTCAGG AGGAGACAGA GGACCCATCT
    GTTATTGGGG AGTTTAAGGG CCTCTTCAAA ATCTATCCCC TCCCAGAAGA CCCGGCTGTT
    CCCATGCCCC CGAGACAATT CCACCAGCTG GCTGCCCAAG GGCCTCAGGA GTGCCTGGTC
    CGCATCTATA TCATCCGAGC ATTTGGCCTT CAGCCCAAGG ACCCCAATGG GAAGTGTGAT
    CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATTCCC
    TGTACTCTGG AGCCTGTGTT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG
    AAGGACCTGA AGATCACACT CTATGACTAT GACCTCCTCT CCAAAGATGA AAAGATTGGG
    GAGACAGTCA TTGACCTGGA GGATAGGCTG CTGTCCAAGT TTGGGGCTCG CTGCGGACTC
    CCACAGACGT ACTGTGTCTC TGGACCCAAC CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG
    CTTCTCCACC TCTTCTGCCA GCAGCGCAGA CTCAAGGCTC CTGTGTACCT CACGGACCAC
    GTGGTGTTCC AGGATAAAGA ATACAGCATT GAAGAAATAG AGGCTGGCCG AGTCCCGAAC
    CCACACCTGG GCCCAGTGGA AGAGCGTCTG GCCTTGCATG TCCTCCAGCA GCAGGGCCTG
    GTCCCTGAGC ATGTGGAGTC CCGGCCTCTC TACAGCCCCC TGCAGCCAGA CATTGAACAG
    GGGAAGCTGC AGATGTGGAT CGACCTATTT CCCAAGGCCC TGGGGCGTCC CGGACCTCCC
    TTCAACATCA CCCCGCGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATCAT CTGGAACACA
    AAGGACGTGA TCCTGGACGA CCTCAGCATC ACAGGGGAGA AGATGAGTGA TATTTATGTG
    AAGGGTTGGA TGGTTGGCTT TGAAGAGCAC AAGCAAAAGA CAGATGTGCA TTACCGGTCC
    CTGGGAGGTG AAGGCAACTT TAACTGGAGG TTCATTTTCC CCTTTGACTA CCTGCCAGCC
    GAGCAAGTCT GCACCGTCTC TAAGAAGGAT GCTTTCTGGA GGCTGGACAA GACTGAGAGC
    AAAATCCCAG CACGAGTGGT ATTTCAGATC TGGGACAATG ACAAGTTCTC CTTTGACGAC
    TTTCTGGGCT CCTTGCAGTT GGATCTCAAC CACATGCCCA AGCCTGCCAA GACAGCTGAG
    AAGTGCTCCT TGGACCAGCT GGATGACACT TTCCACCCGG AGTGGTTTGT GTCCCTTTTT
    GAACAGAAAA CAGTTAAGGG CTGGTGGCCC TGCGTAGCAG AAGAGGGCGA GAAGAAGATC
    CTGGCAGGCA AGCTGGAAAT GACCTTGGAG ATCGTAGCAG AGAGTGAGCA TGAGGAGCGG
    CCTGCTGGCC AGGGCCGGGA TGAACCCAAC ATGAACCCCA AGCTCGAGGA CCCAAGGCGC
    CCTGACACCT CCTTCCTCTG GTTCACCTCT CCATACAAGA CTATGAAGTT CATCCTGTGG
    CGGCGGTTCC GATGTGCCAT TATCCTCTTC ATCATTCTCT TCATCCTGCT ACTATTCTTA
    GGCATCTTCA TCTATGCTTT CCCGAACTAC GCTGCCATGA AGCTGGTGAA GCCCTTCAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe19213
    Clone ID Related Accession (Same CDS sequence) XM_003432228.3 , XM_003432228.4
    Accession Version XM_003432228.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6303bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product dysferlin isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003432228.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC
    CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC
    TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA
    CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGGTG GGGGACAGAG CCGGGCTGAG
    ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GATACAAGAT ACTCTGGAAA GAAGTGGCTG
    GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC TGGAGACGAG
    GGAGAGCCAT TACTGGATCA GAGTGGCGTC CCAGGCCCTG GGGCTCCCAC CACCCCGAGG
    AAGCCACCTT CCCATCTGCC CCCCTACCAC CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC
    GCAGCCAGAA AGCTGCTGTC CGACAAACCG CAGGACTTCC AGATCAGAGT GCAAGTGATC
    GAGGGCCGCC AGCTGCCAGG GGTGAATATC AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG
    CAGACCAAGA GGACACGGAT CCACAAGGGA AACAGCCCAC TATTTAACGA GACTCTTTTC
    TTCAATGTGT TTGACTCTCC TTCGGAGCTG TTTGATGAGC CCATCTTTAT CACGGTCGTA
    GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC
    ATCTACAGAG AGCCCCGACA TGCTTATCTG AGGAAGTGGC TGCTACTCTC CAACCCTGAT
    GACTTCTCTG TTGGGGCCAG AGGTTACCTG AAAGCAAGCC TTTATGTGCT GGGGCCCGGA
    GATGAAGCGC CTCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT TGAAAACAAT
    CTGCTCAGGC CAACTGGCGT GGCCCTGCGA GGAGCCCACT TCTGCCTGAA GATCTTCAGA
    GCGGAGGACT TACCACAAAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC
    TTTGACAGCA ACAAGAAGAA TTTGGTAGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA
    ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACT
    CTGCCTGCCA TGTTTCCCTC CATGTGTGAA AAAATGAAGA TTCGCGTCAT GGACTGGGAC
    CGCCTTACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC
    CCTGGAGGAG AAATAGAAGT GGATGACCAT CTGGGCTTCC TTCCCACCTT CGGGCCCTGC
    TTCGTCAATC TCTATGGCAG CCCCAGGGAA TTCACTGGCT TCCCGGACCC CTACGCGGAA
    CTCAACATGG GCAAGGGGGA AGGCGTGGCC TACCGAGGTC GGCTTCTGCT CTCCCTGGAG
    ACCAAGCTGG TGGAGCACAG CGGCGAGCAA AGGGTGGAGG ACCTCCCTGC TGATGACATC
    CTTCGGGTGG AGAAATACCT CCGGAGGCGC AAGTACTCTC TCTTTGCCGC CTTCTACTCG
    GCCACCATGC TCCAGGATGT CGACGATGCC ATCCAGTTTG AGGTCAGCAT TGGGAACTAT
    GGCAACAAGT TCGACACGAC CTGTCTGCCA CTGGCCTCCA CCACCCAGTA CAGCCGGGCA
    GTCTTTGATG GGTGCCACTA CTACTACTTG CCCTGGGGCA ATGTGAAGCC TGTGGTGGTG
    CTGTCATCCT ACTGGGAAGA CATCAGCCAC AGAGTCGAGG CTCAGAACCA GCTGCTCAGG
    ATCGCTGACC ATCTGGAAGC TGGCCTGGAG CAAGTCCACT TGGCTTTAAA GGCGCAGTTC
    AACACAGAGG ATGTGGACTC CCTGTTGGCC CAGCTGATGG ATGAGCTCAT CTCAGACTGC
    AGCCAGCCCC TGGGCAATGT CCAAGAGATA CCCTCTGCCA CCCACCTGGA CCGGCACCTG
    TACAGGCTGC GCACCCGTCA CCTAGGCCAG ATTACTGAGG CGGCCCTGGC TCTGAAACTC
    GGGCATGGCA AGCTCCCTGC CGCCCTGGAG CAGGCTGAAG ACTGGCTCCT GCGTCTCCGT
    GCCCTGGCTG ATGAGCCCCA GAACAGCCTG CCCGACATCA TCATCTGGAT GCTGCAGGGA
    AACAAGCGAG TGGCGTACCA GCGGGTGCCT GCGCACGAGG TCCTCTTCTC CCGGCGGGGC
    GCCAGCTACT GTGGCAAGAA CTGTGGGAAG CTGCAGACGA TATTTCTGAA ATATCCCATG
    GAGGGGGTGC CCGGAGCCCG GATGCCAGTA CAAATACGGG TGAAACTCTG GTTTGGGCTT
    TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGAA AGCTGTCCGT CTTTGCTGAA
    ACTTATGAGA ACCAGACCAA ACTGGCCCTG GTTGGGAACT GGGGCACAAC AGGCCTCACC
    TACCCCAAGT TTTCTGATGT CATGGGCAAG ATCAAGCTAC CCAAGGATAG CTTCCGCCCC
    TCAGCTGGCT GGACCTGGGC CGGAGACTGG TTCGTGTGTC CAGAGAAGAC CCTACTCCAT
    GACATGGACG CGGGCCACCT GAGTTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTC
    CCTGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACAG ATGTGAATGG GGATAAGGTG
    CTTCCTAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA AGAGTGGTCC
    ACGGACCTCA ATCGGGCCGT CGATGAGCAA GGCTGGGAGT ACAGTATCAC CATCCCCCCG
    GATCGGAAGC CAAAGCACTG GGTTCCTGCT GAGAAGATGT ATTACACGCA CCGTCGGCGG
    CGCTGGGTCC GCCTGCGCAG AAGGGACCTC AGCCAGATGG AGGCATTGAA GAGGCACAGA
    CAGGCAGAGG CAGAGGGTGA GGGCTGGGAG TACGCCTCGC TCTTGGGCTG GAAGTTCCAC
    CTGGAGTACC GCAAGACTGA TGCTTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAACCG
    CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT GCCCTTGAGG GGGCTCTGGG TGGTGTGGTA
    GATGACAGGA GTGAAGATTC CGTGTCCGTG TCCACCTTGA GCTTTGGAGT GAACAGACCC
    ACGATTTCCT GCATCTTTGA TTATGGGAAC CGCTACCACC TACGCTGCTA CATGTACCAG
    GCTCGGGACC TGCCCTCGAT GGACAAGGAC TCTTTTTCTG ATCCCTACGC CATCGTCTCC
    TTCCTGCACC AGAGCCAGAA GACAGTGGTG GCGAAGAACA CTCTGAACCC CACCTGGGAC
    CAGACGCTTA TCTTCTATGA GATTGAGATC TTTGGTGAGC CAGCCAGTAT CGCTGAGCAG
    CCGCCCAGCA TTGTGGTGGA GCTGTATGAC CACGACACCT ACGGTGCAGA TGAGTTTATG
    GGACGCTGCA TTTGTCAGCC CAGCCTGGAG CGGACACCCC GGCTGGCCTG GTTCCCACTG
    ATTAGGGGCA GCCAGTCGGC GGGGGAACTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAA
    AAGCCGGCCA TTCACCATAT TCCTGGTTTT GAGGTACAGG ATATGTCAGG AATCCTGGAA
    GAGTCAGAGG ACATAGACCT GCCCTACCCG CCACCTCAGA GGGAGGCCAA CATCTTCATG
    GTCCCCCAGA ACATCAAGCC TGTTCTCCAG CGTACTGCCA TAGAGATCCT GGCGTGGGGC
    CTGCGGAACA TGAAGAGCTA CCAGCTAGCC AGCATCTCCT CCCCTAGCCT TGTGGTAGAG
    TGCGGGGGCC AGACAGTGCA GTCCTGTGTC ATCAAGAACC TCCGGAAGAA CCCCAACTTC
    GACATCTGTA CCCTTTTCAT GGAAGTGATG CTGCCCAAGG AGGAGGTCTA CTGTCCCTCC
    ATCGTGATCA AGGTCATTGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT
    ACCATCCGTT CCCTGGAGAG CTTCATGTGT GACCTGTACT CCGAGGAGAA TCCGTCCCCA
    CAGGGCGGCC CAGATGATGT GAGTTTACTC AGTCCTGGGG AAGACGTGCT CATTGACATC
    GATGACAAGG AGCCCCTCAT CCCCATGCAG GAGGAAGAGT TCATCGACTG GTGGAGCAAA
    TTCTTTGCCT CCACAGGGGA GAGGGAAAAG TGCGGCTCAT ACTTGGAGAA GGGCTTTGAC
    ACTCTAAAGG TCTATGACAC ACAACTGGAG AACGTGGAGG CCTTTGAGGG CCTGTCTGAC
    TTTTGTAACA CCTTCAAGCT CTACCGGGGC AAGACTCAGG AGGAGACAGA GGACCCATCT
    GTTATTGGGG AGTTTAAGGG CCTCTTCAAA ATCTATCCCC TCCCAGAAGA CCCGGCTGTT
    CCCATGCCCC CGAGACAATT CCACCAGCTG GCTGCCCAAG GGCCTCAGGA GTGCCTGGTC
    CGCATCTATA TCATCCGAGC ATTTGGCCTT CAGCCCAAGG ACCCCAATGG GAAGTGTGAT
    CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATTCCC
    TGTACTCTGG AGCCTGTGTT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG
    AAGGACCTGA AGATCACACT CTATGACTAT GACCTCCTCT CCAAAGATGA AAAGATTGGG
    GAGACAGTCA TTGACCTGGA GGATAGGCTG CTGTCCAAGT TTGGGGCTCG CTGCGGACTC
    CCACAGACGT ACTGTGTCTC TGGACCCAAC CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG
    CTTCTCCACC TCTTCTGCCA GCAGCGCAGA CTCAAGGCTC CTGTGTACCT CACGGACCAC
    GTGGTGTTCC AGGATAAAGA ATACAGCATT GAAGAAATAG AGGCTGGCCG AGTCCCGAAC
    CCACACCTGG GCCCAGTGGA AGAGCGTCTG GCCTTGCATG TCCTCCAGCA GCAGGGCCTG
    GTCCCTGAGC ATGTGGAGTC CCGGCCTCTC TACAGCCCCC TGCAGCCAGA CATTGAACAG
    GGGAAGCTGC AGATGTGGAT CGACCTATTT CCCAAGGCCC TGGGGCGTCC CGGACCTCCC
    TTCAACATCA CCCCGCGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATCAT CTGGAACACA
    AAGGACGTGA TCCTGGACGA CCTCAGCATC ACAGGGGAGA AGATGAGTGA TATTTATGTG
    AAGGGTTGGA TGGTTGGCTT TGAAGAGCAC AAGCAAAAGA CAGATGTGCA TTACCGGTCC
    CTGGGAGGTG AAGGCAACTT TAACTGGAGG TTCATTTTCC CCTTTGACTA CCTGCCAGCC
    GAGCAAGTCT GCACCGTCTC TAAGAAGGAT GCTTTCTGGA GGCTGGACAA GACTGAGAGC
    AAAATCCCAG CACGAGTGGT ATTTCAGATC TGGGACAATG ACAAGTTCTC CTTTGACGAC
    TTTCTGGGCT CCTTGCAGTT GGATCTCAAC CACATGCCCA AGCCTGCCAA GACAGCTGAG
    AAGTGCTCCT TGGACCAGCT GGATGACACT TTCCACCCGG AGTGGTTTGT GTCCCTTTTT
    GAACAGAAAA CAGTTAAGGG CTGGTGGCCC TGCGTAGCAG AAGAGGGCGA GAAGAAGATC
    CTGGCAGGCA AGCTGGAAAT GACCTTGGAG ATCGTAGCAG AGAGTGAGCA TGAGGAGCGG
    CCTGCTGGCC AGGGCCGGGA TGAACCCAAC ATGAACCCCA AGCTCGAGGA CCCAAGGCGC
    CCTGACACCT CCTTCCTCTG GTTCACCTCT CCATACAAGA CTATGAAGTT CATCCTGTGG
    CGGCGGTTCC GATGTGCCAT TATCCTCTTC ATCATTCTCT TCATCCTGCT ACTATTCTTA
    GGCATCTTCA TCTATGCTTT CCCGAACTAC GCTGCCATGA AGCTGGTGAA GCCCTTCAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003432276.1
    CDS405..6707
    Translation

    Target ORF information:

    RefSeq Version XM_003432228.4
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris dysferlin (DYSF), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003432228.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC
    CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC
    TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA
    CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGGTG GGGGACAGAG CCGGGCTGAG
    ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GATACAAGAT ACTCTGGAAA GAAGTGGCTG
    GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC TGGAGACGAG
    GGAGAGCCAT TACTGGATCA GAGTGGCGTC CCAGGCCCTG GGGCTCCCAC CACCCCGAGG
    AAGCCACCTT CCCATCTGCC CCCCTACCAC CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC
    GCAGCCAGAA AGCTGCTGTC CGACAAACCG CAGGACTTCC AGATCAGAGT GCAAGTGATC
    GAGGGCCGCC AGCTGCCAGG GGTGAATATC AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG
    CAGACCAAGA GGACACGGAT CCACAAGGGA AACAGCCCAC TATTTAACGA GACTCTTTTC
    TTCAATGTGT TTGACTCTCC TTCGGAGCTG TTTGATGAGC CCATCTTTAT CACGGTCGTA
    GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC
    ATCTACAGAG AGCCCCGACA TGCTTATCTG AGGAAGTGGC TGCTACTCTC CAACCCTGAT
    GACTTCTCTG TTGGGGCCAG AGGTTACCTG AAAGCAAGCC TTTATGTGCT GGGGCCCGGA
    GATGAAGCGC CTCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT TGAAAACAAT
    CTGCTCAGGC CAACTGGCGT GGCCCTGCGA GGAGCCCACT TCTGCCTGAA GATCTTCAGA
    GCGGAGGACT TACCACAAAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC
    TTTGACAGCA ACAAGAAGAA TTTGGTAGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA
    ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACT
    CTGCCTGCCA TGTTTCCCTC CATGTGTGAA AAAATGAAGA TTCGCGTCAT GGACTGGGAC
    CGCCTTACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC
    CCTGGAGGAG AAATAGAAGT GGATGACCAT CTGGGCTTCC TTCCCACCTT CGGGCCCTGC
    TTCGTCAATC TCTATGGCAG CCCCAGGGAA TTCACTGGCT TCCCGGACCC CTACGCGGAA
    CTCAACATGG GCAAGGGGGA AGGCGTGGCC TACCGAGGTC GGCTTCTGCT CTCCCTGGAG
    ACCAAGCTGG TGGAGCACAG CGGCGAGCAA AGGGTGGAGG ACCTCCCTGC TGATGACATC
    CTTCGGGTGG AGAAATACCT CCGGAGGCGC AAGTACTCTC TCTTTGCCGC CTTCTACTCG
    GCCACCATGC TCCAGGATGT CGACGATGCC ATCCAGTTTG AGGTCAGCAT TGGGAACTAT
    GGCAACAAGT TCGACACGAC CTGTCTGCCA CTGGCCTCCA CCACCCAGTA CAGCCGGGCA
    GTCTTTGATG GGTGCCACTA CTACTACTTG CCCTGGGGCA ATGTGAAGCC TGTGGTGGTG
    CTGTCATCCT ACTGGGAAGA CATCAGCCAC AGAGTCGAGG CTCAGAACCA GCTGCTCAGG
    ATCGCTGACC ATCTGGAAGC TGGCCTGGAG CAAGTCCACT TGGCTTTAAA GGCGCAGTTC
    AACACAGAGG ATGTGGACTC CCTGTTGGCC CAGCTGATGG ATGAGCTCAT CTCAGACTGC
    AGCCAGCCCC TGGGCAATGT CCAAGAGATA CCCTCTGCCA CCCACCTGGA CCGGCACCTG
    TACAGGCTGC GCACCCGTCA CCTAGGCCAG ATTACTGAGG CGGCCCTGGC TCTGAAACTC
    GGGCATGGCA AGCTCCCTGC CGCCCTGGAG CAGGCTGAAG ACTGGCTCCT GCGTCTCCGT
    GCCCTGGCTG ATGAGCCCCA GAACAGCCTG CCCGACATCA TCATCTGGAT GCTGCAGGGA
    AACAAGCGAG TGGCGTACCA GCGGGTGCCT GCGCACGAGG TCCTCTTCTC CCGGCGGGGC
    GCCAGCTACT GTGGCAAGAA CTGTGGGAAG CTGCAGACGA TATTTCTGAA ATATCCCATG
    GAGGGGGTGC CCGGAGCCCG GATGCCAGTA CAAATACGGG TGAAACTCTG GTTTGGGCTT
    TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGAA AGCTGTCCGT CTTTGCTGAA
    ACTTATGAGA ACCAGACCAA ACTGGCCCTG GTTGGGAACT GGGGCACAAC AGGCCTCACC
    TACCCCAAGT TTTCTGATGT CATGGGCAAG ATCAAGCTAC CCAAGGATAG CTTCCGCCCC
    TCAGCTGGCT GGACCTGGGC CGGAGACTGG TTCGTGTGTC CAGAGAAGAC CCTACTCCAT
    GACATGGACG CGGGCCACCT GAGTTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTC
    CCTGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACAG ATGTGAATGG GGATAAGGTG
    CTTCCTAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA AGAGTGGTCC
    ACGGACCTCA ATCGGGCCGT CGATGAGCAA GGCTGGGAGT ACAGTATCAC CATCCCCCCG
    GATCGGAAGC CAAAGCACTG GGTTCCTGCT GAGAAGATGT ATTACACGCA CCGTCGGCGG
    CGCTGGGTCC GCCTGCGCAG AAGGGACCTC AGCCAGATGG AGGCATTGAA GAGGCACAGA
    CAGGCAGAGG CAGAGGGTGA GGGCTGGGAG TACGCCTCGC TCTTGGGCTG GAAGTTCCAC
    CTGGAGTACC GCAAGACTGA TGCTTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAACCG
    CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT GCCCTTGAGG GGGCTCTGGG TGGTGTGGTA
    GATGACAGGA GTGAAGATTC CGTGTCCGTG TCCACCTTGA GCTTTGGAGT GAACAGACCC
    ACGATTTCCT GCATCTTTGA TTATGGGAAC CGCTACCACC TACGCTGCTA CATGTACCAG
    GCTCGGGACC TGCCCTCGAT GGACAAGGAC TCTTTTTCTG ATCCCTACGC CATCGTCTCC
    TTCCTGCACC AGAGCCAGAA GACAGTGGTG GCGAAGAACA CTCTGAACCC CACCTGGGAC
    CAGACGCTTA TCTTCTATGA GATTGAGATC TTTGGTGAGC CAGCCAGTAT CGCTGAGCAG
    CCGCCCAGCA TTGTGGTGGA GCTGTATGAC CACGACACCT ACGGTGCAGA TGAGTTTATG
    GGACGCTGCA TTTGTCAGCC CAGCCTGGAG CGGACACCCC GGCTGGCCTG GTTCCCACTG
    ATTAGGGGCA GCCAGTCGGC GGGGGAACTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAA
    AAGCCGGCCA TTCACCATAT TCCTGGTTTT GAGGTACAGG ATATGTCAGG AATCCTGGAA
    GAGTCAGAGG ACATAGACCT GCCCTACCCG CCACCTCAGA GGGAGGCCAA CATCTTCATG
    GTCCCCCAGA ACATCAAGCC TGTTCTCCAG CGTACTGCCA TAGAGATCCT GGCGTGGGGC
    CTGCGGAACA TGAAGAGCTA CCAGCTAGCC AGCATCTCCT CCCCTAGCCT TGTGGTAGAG
    TGCGGGGGCC AGACAGTGCA GTCCTGTGTC ATCAAGAACC TCCGGAAGAA CCCCAACTTC
    GACATCTGTA CCCTTTTCAT GGAAGTGATG CTGCCCAAGG AGGAGGTCTA CTGTCCCTCC
    ATCGTGATCA AGGTCATTGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT
    ACCATCCGTT CCCTGGAGAG CTTCATGTGT GACCTGTACT CCGAGGAGAA TCCGTCCCCA
    CAGGGCGGCC CAGATGATGT GAGTTTACTC AGTCCTGGGG AAGACGTGCT CATTGACATC
    GATGACAAGG AGCCCCTCAT CCCCATGCAG GAGGAAGAGT TCATCGACTG GTGGAGCAAA
    TTCTTTGCCT CCACAGGGGA GAGGGAAAAG TGCGGCTCAT ACTTGGAGAA GGGCTTTGAC
    ACTCTAAAGG TCTATGACAC ACAACTGGAG AACGTGGAGG CCTTTGAGGG CCTGTCTGAC
    TTTTGTAACA CCTTCAAGCT CTACCGGGGC AAGACTCAGG AGGAGACAGA GGACCCATCT
    GTTATTGGGG AGTTTAAGGG CCTCTTCAAA ATCTATCCCC TCCCAGAAGA CCCGGCTGTT
    CCCATGCCCC CGAGACAATT CCACCAGCTG GCTGCCCAAG GGCCTCAGGA GTGCCTGGTC
    CGCATCTATA TCATCCGAGC ATTTGGCCTT CAGCCCAAGG ACCCCAATGG GAAGTGTGAT
    CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATTCCC
    TGTACTCTGG AGCCTGTGTT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG
    AAGGACCTGA AGATCACACT CTATGACTAT GACCTCCTCT CCAAAGATGA AAAGATTGGG
    GAGACAGTCA TTGACCTGGA GGATAGGCTG CTGTCCAAGT TTGGGGCTCG CTGCGGACTC
    CCACAGACGT ACTGTGTCTC TGGACCCAAC CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG
    CTTCTCCACC TCTTCTGCCA GCAGCGCAGA CTCAAGGCTC CTGTGTACCT CACGGACCAC
    GTGGTGTTCC AGGATAAAGA ATACAGCATT GAAGAAATAG AGGCTGGCCG AGTCCCGAAC
    CCACACCTGG GCCCAGTGGA AGAGCGTCTG GCCTTGCATG TCCTCCAGCA GCAGGGCCTG
    GTCCCTGAGC ATGTGGAGTC CCGGCCTCTC TACAGCCCCC TGCAGCCAGA CATTGAACAG
    GGGAAGCTGC AGATGTGGAT CGACCTATTT CCCAAGGCCC TGGGGCGTCC CGGACCTCCC
    TTCAACATCA CCCCGCGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATCAT CTGGAACACA
    AAGGACGTGA TCCTGGACGA CCTCAGCATC ACAGGGGAGA AGATGAGTGA TATTTATGTG
    AAGGGTTGGA TGGTTGGCTT TGAAGAGCAC AAGCAAAAGA CAGATGTGCA TTACCGGTCC
    CTGGGAGGTG AAGGCAACTT TAACTGGAGG TTCATTTTCC CCTTTGACTA CCTGCCAGCC
    GAGCAAGTCT GCACCGTCTC TAAGAAGGAT GCTTTCTGGA GGCTGGACAA GACTGAGAGC
    AAAATCCCAG CACGAGTGGT ATTTCAGATC TGGGACAATG ACAAGTTCTC CTTTGACGAC
    TTTCTGGGCT CCTTGCAGTT GGATCTCAAC CACATGCCCA AGCCTGCCAA GACAGCTGAG
    AAGTGCTCCT TGGACCAGCT GGATGACACT TTCCACCCGG AGTGGTTTGT GTCCCTTTTT
    GAACAGAAAA CAGTTAAGGG CTGGTGGCCC TGCGTAGCAG AAGAGGGCGA GAAGAAGATC
    CTGGCAGGCA AGCTGGAAAT GACCTTGGAG ATCGTAGCAG AGAGTGAGCA TGAGGAGCGG
    CCTGCTGGCC AGGGCCGGGA TGAACCCAAC ATGAACCCCA AGCTCGAGGA CCCAAGGCGC
    CCTGACACCT CCTTCCTCTG GTTCACCTCT CCATACAAGA CTATGAAGTT CATCCTGTGG
    CGGCGGTTCC GATGTGCCAT TATCCTCTTC ATCATTCTCT TCATCCTGCT ACTATTCTTA
    GGCATCTTCA TCTATGCTTT CCCGAACTAC GCTGCCATGA AGCTGGTGAA GCCCTTCAGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe19214
    Clone ID Related Accession (Same CDS sequence) XM_003432231.4 , XM_003432231.3
    Accession Version XM_003432231.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6276bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product dysferlin isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003432231.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG ACACAGGAGG AGAGGAGGAC
    ACTGAGGACC AGGGGCTCAC TGGAGACGAG GGAGAGCCAT TACTGGATCA GAGTGGCGTC
    CCAGGCCCTG GGGCTCCCAC CACCCCGAGG AAGCCACCTT CCCATCTGCC CCCCTACCAC
    CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC GCAGCCAGAA AGCTGCTGTC CGACAAACCG
    CAGGACTTCC AGATCAGAGT GCAAGTGATC GAGGGCCGCC AGCTGCCAGG GGTGAATATC
    AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG CAGACCAAGA GGACACGGAT CCACAAGGGA
    AACAGCCCAC TATTTAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TTCGGAGCTG
    TTTGATGAGC CCATCTTTAT CACGGTCGTA GACTCCCGTT CTCTCAGGAC AGATGCTCTC
    ATTGGGGAAT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA TGCTTATCTG
    AGGAAGTGGC TGCTACTCTC CAACCCTGAT GACTTCTCTG TTGGGGCCAG AGGTTACCTG
    AAAGCAAGCC TTTATGTGCT GGGGCCCGGA GATGAAGCGC CTCTGGAGAG AAAGGACCCC
    TCTGAAGACA AGGAGGACAT TGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTGCGA
    GGAGCCCACT TCTGCCTGAA GATCTTCAGA GCGGAGGACT TACCACAAAT GGATGATGCC
    GTGATGGACA ATGTGAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA TTTGGTAGAT
    CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA
    GCCAACCCTC AGTGGAACCA GAGCATCACT CTGCCTGCCA TGTTTCCCTC CATGTGTGAA
    AAAATGAAGA TTCGCGTCAT GGACTGGGAC CGCCTTACCC ACAATGACAT CGTGGCCACC
    ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGAAGT GGATGACCAT
    CTGGGCTTCC TTCCCACCTT CGGGCCCTGC TTCGTCAATC TCTATGGCAG CCCCAGGGAA
    TTCACTGGCT TCCCGGACCC CTACGCGGAA CTCAACATGG GCAAGGGGGA AGGCGTGGCC
    TACCGAGGTC GGCTTCTGCT CTCCCTGGAG ACCAAGCTGG TGGAGCACAG CGGCGAGCAA
    AGGGTGGAGG ACCTCCCTGC TGATGACATC CTTCGGGTGG AGAAATACCT CCGGAGGCGC
    AAGTACTCTC TCTTTGCCGC CTTCTACTCG GCCACCATGC TCCAGGATGT CGACGATGCC
    ATCCAGTTTG AGGTCAGCAT TGGGAACTAT GGCAACAAGT TCGACACGAC CTGTCTGCCA
    CTGGCCTCCA CCACCCAGTA CAGCCGGGCA GTCTTTGATG GGTGCCACTA CTACTACTTG
    CCCTGGGGCA ATGTGAAGCC TGTGGTGGTG CTGTCATCCT ACTGGGAAGA CATCAGCCAC
    AGAGTCGAGG CTCAGAACCA GCTGCTCAGG ATCGCTGACC ATCTGGAAGC TGGCCTGGAG
    CAAGTCCACT TGGCTTTAAA GGCGCAGTTC AACACAGAGG ATGTGGACTC CCTGTTGGCC
    CAGCTGATGG ATGAGCTCAT CTCAGACTGC AGCCAGCCCC TGGGCAATGT CCAAGAGATA
    CCCTCTGCCA CCCACCTGGA CCGGCACCTG TACAGGCTGC GCACCCGTCA CCTAGGCCAG
    ATTACTGAGG CGGCCCTGGC TCTGAAACTC GGGCATGGCA AGCTCCCTGC CGCCCTGGAG
    CAGGCTGAAG ACTGGCTCCT GCGTCTCCGT GCCCTGGCTG ATGAGCCCCA GAACAGCCTG
    CCCGACATCA TCATCTGGAT GCTGCAGGGA AACAAGCGAG TGGCGTACCA GCGGGTGCCT
    GCGCACGAGG TCCTCTTCTC CCGGCGGGGC GCCAGCTACT GTGGCAAGAA CTGTGGGAAG
    CTGCAGACGA TATTTCTGAA ATATCCCATG GAGGGGGTGC CCGGAGCCCG GATGCCAGTA
    CAAATACGGG TGAAACTCTG GTTTGGGCTT TCTGTGGATG AGAAGGAGTT CAACCAGTTT
    GCTGAGGGAA AGCTGTCCGT CTTTGCTGAA ACTTATGAGA ACCAGACCAA ACTGGCCCTG
    GTTGGGAACT GGGGCACAAC AGGCCTCACC TACCCCAAGT TTTCTGATGT CATGGGCAAG
    ATCAAGCTAC CCAAGGATAG CTTCCGCCCC TCAGCTGGCT GGACCTGGGC CGGAGACTGG
    TTCGTGTGTC CAGAGAAGAC CCTACTCCAT GACATGGACG CGGGCCACCT GAGTTTTGTG
    GAGGAGGTGT TTGAGAACCA GACCCGGCTC CCTGGAGGCC AGTGGATCTA CATGAGTGAC
    AACTACACAG ATGTGAATGG GGATAAGGTG CTTCCTAAGG ATGACATTGA GTGCCCACTG
    GGCTGGAAGT GGGAAGATGA AGAGTGGTCC ACGGACCTCA ATCGGGCCGT CGATGAGCAA
    GGCTGGGAGT ACAGTATCAC CATCCCCCCG GATCGGAAGC CAAAGCACTG GGTTCCTGCT
    GAGAAGATGT ATTACACGCA CCGTCGGCGG CGCTGGGTCC GCCTGCGCAG AAGGGACCTC
    AGCCAGATGG AGGCATTGAA GAGGCACAGA CAGGCAGAGG CAGAGGGTGA GGGCTGGGAG
    TACGCCTCGC TCTTGGGCTG GAAGTTCCAC CTGGAGTACC GCAAGACTGA TGCTTTCCGC
    CGCCGCCGCT GGCGCCGCCG CATGGAACCG CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT
    GCCCTTGAGG GGGCTCTGGG TGGTGTGGTA GATGACAGGA GTGAAGATTC CGTGTCCGTG
    TCCACCTTGA GCTTTGGAGT GAACAGACCC ACGATTTCCT GCATCTTTGA TTATGGGAAC
    CGCTACCACC TACGCTGCTA CATGTACCAG GCTCGGGACC TGCCCTCGAT GGACAAGGAC
    TCTTTTTCTG ATCCCTACGC CATCGTCTCC TTCCTGCACC AGAGCCAGAA GACAGTGGTG
    GCGAAGAACA CTCTGAACCC CACCTGGGAC CAGACGCTTA TCTTCTATGA GATTGAGATC
    TTTGGTGAGC CAGCCAGTAT CGCTGAGCAG CCGCCCAGCA TTGTGGTGGA GCTGTATGAC
    CACGACACCT ACGGTGCAGA TGAGTTTATG GGACGCTGCA TTTGTCAGCC CAGCCTGGAG
    CGGACACCCC GGCTGGCCTG GTTCCCACTG ATTAGGGGCA GCCAGTCGGC GGGGGAACTG
    CTGGCCTCTT TTGAGCTCAT CCAGAGAGAA AAGCCGGCCA TTCACCATAT TCCTGGTTTT
    GAGGTACAGG ATATGTCAGG AATCCTGGAA GAGTCAGAGG ACATAGACCT GCCCTACCCG
    CCACCTCAGA GGGAGGCCAA CATCTTCATG GTCCCCCAGA ACATCAAGCC TGTTCTCCAG
    CGTACTGCCA TAGAGATCCT GGCGTGGGGC CTGCGGAACA TGAAGAGCTA CCAGCTAGCC
    AGCATCTCCT CCCCTAGCCT TGTGGTAGAG TGCGGGGGCC AGACAGTGCA GTCCTGTGTC
    ATCAAGAACC TCCGGAAGAA CCCCAACTTC GACATCTGTA CCCTTTTCAT GGAAGTGATG
    CTGCCCAAGG AGGAGGTCTA CTGTCCCTCC ATCGTGATCA AGGTCATTGA TAACCGCCAG
    TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT ACCATCCGTT CCCTGGAGAG CTTCATGTGT
    GACCTGTACT CCGAGGAGAA TCCGTCCCCA CAGGGCGGCC CAGATGATGT GAGTTTACTC
    AGTCCTGGGG AAGACGTGCT CATTGACATC GATGACAAGG AGCCCCTCAT CCCCATGCAG
    CTTGCAGATG GTCTGTCGGG CTCTGCCCCC ACTAACATGG CATCTTCTCC ATCCAGTCCT
    CATGAGGAAG AGTTCATCGA CTGGTGGAGC AAATTCTTTG CCTCCACAGG GGAGAGGGAA
    AAGTGCGGCT CATACTTGGA GAAGGGCTTT GACACTCTAA AGGTCTATGA CACACAACTG
    GAGAACGTGG AGGCCTTTGA GGGCCTGTCT GACTTTTGTA ACACCTTCAA GCTCTACCGG
    GGCAAGACTC AGGAGGAGAC AGAGGACCCA TCTGTTATTG GGGAGTTTAA GGGCCTCTTC
    AAAATCTATC CCCTCCCAGA AGACCCGGCT GTTCCCATGC CCCCGAGACA ATTCCACCAG
    CTGGCTGCCC AAGGGCCTCA GGAGTGCCTG GTCCGCATCT ATATCATCCG AGCATTTGGC
    CTTCAGCCCA AGGACCCCAA TGGGAAGTGT GATCCTTACA TCAAGATCTC CATAGGGAAG
    AAATCAGTGA GTGACCAGGA TAACTACATT CCCTGTACTC TGGAGCCTGT GTTTGGAAAG
    ATGTTCGAGC TGACCTGCAC TCTGCCTCTG GAGAAGGACC TGAAGATCAC ACTCTATGAC
    TATGACCTCC TCTCCAAAGA TGAAAAGATT GGGGAGACAG TCATTGACCT GGAGGATAGG
    CTGCTGTCCA AGTTTGGGGC TCGCTGCGGA CTCCCACAGA CGTACTGTGT CTCTGGACCC
    AACCAGTGGA GGGACCAGCT CCGCCCCTCC CAGCTTCTCC ACCTCTTCTG CCAGCAGCGC
    AGACTCAAGG CTCCTGTGTA CCTCACGGAC CACGTGGTGT TCCAGGATAA AGAATACAGC
    ATTGAAGAAA TAGAGGCTGG CCGAGTCCCG AACCCACACC TGGGCCCAGT GGAAGAGCGT
    CTGGCCTTGC ATGTCCTCCA GCAGCAGGGC CTGGTCCCTG AGCATGTGGA GTCCCGGCCT
    CTCTACAGCC CCCTGCAGCC AGACATTGAA CAGGGGAAGC TGCAGATGTG GATCGACCTA
    TTTCCCAAGG CCCTGGGGCG TCCCGGACCT CCCTTCAACA TCACCCCGCG GAGAGCCAGA
    AGGTTTTTCC TGCGTTGTAT CATCTGGAAC ACAAAGGACG TGATCCTGGA CGACCTCAGC
    ATCACAGGGG AGAAGATGAG TGATATTTAT GTGAAGGGTT GGATGGTTGG CTTTGAAGAG
    CACAAGCAAA AGACAGATGT GCATTACCGG TCCCTGGGAG GTGAAGGCAA CTTTAACTGG
    AGGTTCATTT TCCCCTTTGA CTACCTGCCA GCCGAGCAAG TCTGCACCGT CTCTAAGAAG
    GATGCTTTCT GGAGGCTGGA CAAGACTGAG AGCAAAATCC CAGCACGAGT GGTATTTCAG
    ATCTGGGACA ATGACAAGTT CTCCTTTGAC GACTTTCTGG GCTCCTTGCA GTTGGATCTC
    AACCACATGC CCAAGCCTGC CAAGACAGCT GAGAAGTGCT CCTTGGACCA GCTGGATGAC
    ACTTTCCACC CGGAGTGGTT TGTGTCCCTT TTTGAACAGA AAACAGTTAA GGGCTGGTGG
    CCCTGCGTAG CAGAAGAGGG CGAGAAGAAG ATCCTGGCAG GCAAGCTGGA AATGACCTTG
    GAGATCGTAG CAGAGAGTGA GCATGAGGAG CGGCCTGCTG GCCAGGGCCG GGATGAACCC
    AACATGAACC CCAAGCTCGA GGACCCAAGG CGCCCTGACA CCTCCTTCCT CTGGTTCACC
    TCTCCATACA AGACTATGAA GTTCATCCTG TGGCGGCGGT TCCGATGTGC CATTATCCTC
    TTCATCATTC TCTTCATCCT GCTACTATTC TTAGGCATCT TCATCTATGC TTTCCCGAAC
    TACGCTGCCA TGAAGCTGGT GAAGCCCTTC AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003432279.1
    CDS23..6298
    Translation

    Target ORF information:

    RefSeq Version XM_003432231.4
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris dysferlin (DYSF), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003432231.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG ACACAGGAGG AGAGGAGGAC
    ACTGAGGACC AGGGGCTCAC TGGAGACGAG GGAGAGCCAT TACTGGATCA GAGTGGCGTC
    CCAGGCCCTG GGGCTCCCAC CACCCCGAGG AAGCCACCTT CCCATCTGCC CCCCTACCAC
    CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC GCAGCCAGAA AGCTGCTGTC CGACAAACCG
    CAGGACTTCC AGATCAGAGT GCAAGTGATC GAGGGCCGCC AGCTGCCAGG GGTGAATATC
    AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG CAGACCAAGA GGACACGGAT CCACAAGGGA
    AACAGCCCAC TATTTAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TTCGGAGCTG
    TTTGATGAGC CCATCTTTAT CACGGTCGTA GACTCCCGTT CTCTCAGGAC AGATGCTCTC
    ATTGGGGAAT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA TGCTTATCTG
    AGGAAGTGGC TGCTACTCTC CAACCCTGAT GACTTCTCTG TTGGGGCCAG AGGTTACCTG
    AAAGCAAGCC TTTATGTGCT GGGGCCCGGA GATGAAGCGC CTCTGGAGAG AAAGGACCCC
    TCTGAAGACA AGGAGGACAT TGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTGCGA
    GGAGCCCACT TCTGCCTGAA GATCTTCAGA GCGGAGGACT TACCACAAAT GGATGATGCC
    GTGATGGACA ATGTGAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA TTTGGTAGAT
    CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA
    GCCAACCCTC AGTGGAACCA GAGCATCACT CTGCCTGCCA TGTTTCCCTC CATGTGTGAA
    AAAATGAAGA TTCGCGTCAT GGACTGGGAC CGCCTTACCC ACAATGACAT CGTGGCCACC
    ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGAAGT GGATGACCAT
    CTGGGCTTCC TTCCCACCTT CGGGCCCTGC TTCGTCAATC TCTATGGCAG CCCCAGGGAA
    TTCACTGGCT TCCCGGACCC CTACGCGGAA CTCAACATGG GCAAGGGGGA AGGCGTGGCC
    TACCGAGGTC GGCTTCTGCT CTCCCTGGAG ACCAAGCTGG TGGAGCACAG CGGCGAGCAA
    AGGGTGGAGG ACCTCCCTGC TGATGACATC CTTCGGGTGG AGAAATACCT CCGGAGGCGC
    AAGTACTCTC TCTTTGCCGC CTTCTACTCG GCCACCATGC TCCAGGATGT CGACGATGCC
    ATCCAGTTTG AGGTCAGCAT TGGGAACTAT GGCAACAAGT TCGACACGAC CTGTCTGCCA
    CTGGCCTCCA CCACCCAGTA CAGCCGGGCA GTCTTTGATG GGTGCCACTA CTACTACTTG
    CCCTGGGGCA ATGTGAAGCC TGTGGTGGTG CTGTCATCCT ACTGGGAAGA CATCAGCCAC
    AGAGTCGAGG CTCAGAACCA GCTGCTCAGG ATCGCTGACC ATCTGGAAGC TGGCCTGGAG
    CAAGTCCACT TGGCTTTAAA GGCGCAGTTC AACACAGAGG ATGTGGACTC CCTGTTGGCC
    CAGCTGATGG ATGAGCTCAT CTCAGACTGC AGCCAGCCCC TGGGCAATGT CCAAGAGATA
    CCCTCTGCCA CCCACCTGGA CCGGCACCTG TACAGGCTGC GCACCCGTCA CCTAGGCCAG
    ATTACTGAGG CGGCCCTGGC TCTGAAACTC GGGCATGGCA AGCTCCCTGC CGCCCTGGAG
    CAGGCTGAAG ACTGGCTCCT GCGTCTCCGT GCCCTGGCTG ATGAGCCCCA GAACAGCCTG
    CCCGACATCA TCATCTGGAT GCTGCAGGGA AACAAGCGAG TGGCGTACCA GCGGGTGCCT
    GCGCACGAGG TCCTCTTCTC CCGGCGGGGC GCCAGCTACT GTGGCAAGAA CTGTGGGAAG
    CTGCAGACGA TATTTCTGAA ATATCCCATG GAGGGGGTGC CCGGAGCCCG GATGCCAGTA
    CAAATACGGG TGAAACTCTG GTTTGGGCTT TCTGTGGATG AGAAGGAGTT CAACCAGTTT
    GCTGAGGGAA AGCTGTCCGT CTTTGCTGAA ACTTATGAGA ACCAGACCAA ACTGGCCCTG
    GTTGGGAACT GGGGCACAAC AGGCCTCACC TACCCCAAGT TTTCTGATGT CATGGGCAAG
    ATCAAGCTAC CCAAGGATAG CTTCCGCCCC TCAGCTGGCT GGACCTGGGC CGGAGACTGG
    TTCGTGTGTC CAGAGAAGAC CCTACTCCAT GACATGGACG CGGGCCACCT GAGTTTTGTG
    GAGGAGGTGT TTGAGAACCA GACCCGGCTC CCTGGAGGCC AGTGGATCTA CATGAGTGAC
    AACTACACAG ATGTGAATGG GGATAAGGTG CTTCCTAAGG ATGACATTGA GTGCCCACTG
    GGCTGGAAGT GGGAAGATGA AGAGTGGTCC ACGGACCTCA ATCGGGCCGT CGATGAGCAA
    GGCTGGGAGT ACAGTATCAC CATCCCCCCG GATCGGAAGC CAAAGCACTG GGTTCCTGCT
    GAGAAGATGT ATTACACGCA CCGTCGGCGG CGCTGGGTCC GCCTGCGCAG AAGGGACCTC
    AGCCAGATGG AGGCATTGAA GAGGCACAGA CAGGCAGAGG CAGAGGGTGA GGGCTGGGAG
    TACGCCTCGC TCTTGGGCTG GAAGTTCCAC CTGGAGTACC GCAAGACTGA TGCTTTCCGC
    CGCCGCCGCT GGCGCCGCCG CATGGAACCG CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT
    GCCCTTGAGG GGGCTCTGGG TGGTGTGGTA GATGACAGGA GTGAAGATTC CGTGTCCGTG
    TCCACCTTGA GCTTTGGAGT GAACAGACCC ACGATTTCCT GCATCTTTGA TTATGGGAAC
    CGCTACCACC TACGCTGCTA CATGTACCAG GCTCGGGACC TGCCCTCGAT GGACAAGGAC
    TCTTTTTCTG ATCCCTACGC CATCGTCTCC TTCCTGCACC AGAGCCAGAA GACAGTGGTG
    GCGAAGAACA CTCTGAACCC CACCTGGGAC CAGACGCTTA TCTTCTATGA GATTGAGATC
    TTTGGTGAGC CAGCCAGTAT CGCTGAGCAG CCGCCCAGCA TTGTGGTGGA GCTGTATGAC
    CACGACACCT ACGGTGCAGA TGAGTTTATG GGACGCTGCA TTTGTCAGCC CAGCCTGGAG
    CGGACACCCC GGCTGGCCTG GTTCCCACTG ATTAGGGGCA GCCAGTCGGC GGGGGAACTG
    CTGGCCTCTT TTGAGCTCAT CCAGAGAGAA AAGCCGGCCA TTCACCATAT TCCTGGTTTT
    GAGGTACAGG ATATGTCAGG AATCCTGGAA GAGTCAGAGG ACATAGACCT GCCCTACCCG
    CCACCTCAGA GGGAGGCCAA CATCTTCATG GTCCCCCAGA ACATCAAGCC TGTTCTCCAG
    CGTACTGCCA TAGAGATCCT GGCGTGGGGC CTGCGGAACA TGAAGAGCTA CCAGCTAGCC
    AGCATCTCCT CCCCTAGCCT TGTGGTAGAG TGCGGGGGCC AGACAGTGCA GTCCTGTGTC
    ATCAAGAACC TCCGGAAGAA CCCCAACTTC GACATCTGTA CCCTTTTCAT GGAAGTGATG
    CTGCCCAAGG AGGAGGTCTA CTGTCCCTCC ATCGTGATCA AGGTCATTGA TAACCGCCAG
    TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT ACCATCCGTT CCCTGGAGAG CTTCATGTGT
    GACCTGTACT CCGAGGAGAA TCCGTCCCCA CAGGGCGGCC CAGATGATGT GAGTTTACTC
    AGTCCTGGGG AAGACGTGCT CATTGACATC GATGACAAGG AGCCCCTCAT CCCCATGCAG
    CTTGCAGATG GTCTGTCGGG CTCTGCCCCC ACTAACATGG CATCTTCTCC ATCCAGTCCT
    CATGAGGAAG AGTTCATCGA CTGGTGGAGC AAATTCTTTG CCTCCACAGG GGAGAGGGAA
    AAGTGCGGCT CATACTTGGA GAAGGGCTTT GACACTCTAA AGGTCTATGA CACACAACTG
    GAGAACGTGG AGGCCTTTGA GGGCCTGTCT GACTTTTGTA ACACCTTCAA GCTCTACCGG
    GGCAAGACTC AGGAGGAGAC AGAGGACCCA TCTGTTATTG GGGAGTTTAA GGGCCTCTTC
    AAAATCTATC CCCTCCCAGA AGACCCGGCT GTTCCCATGC CCCCGAGACA ATTCCACCAG
    CTGGCTGCCC AAGGGCCTCA GGAGTGCCTG GTCCGCATCT ATATCATCCG AGCATTTGGC
    CTTCAGCCCA AGGACCCCAA TGGGAAGTGT GATCCTTACA TCAAGATCTC CATAGGGAAG
    AAATCAGTGA GTGACCAGGA TAACTACATT CCCTGTACTC TGGAGCCTGT GTTTGGAAAG
    ATGTTCGAGC TGACCTGCAC TCTGCCTCTG GAGAAGGACC TGAAGATCAC ACTCTATGAC
    TATGACCTCC TCTCCAAAGA TGAAAAGATT GGGGAGACAG TCATTGACCT GGAGGATAGG
    CTGCTGTCCA AGTTTGGGGC TCGCTGCGGA CTCCCACAGA CGTACTGTGT CTCTGGACCC
    AACCAGTGGA GGGACCAGCT CCGCCCCTCC CAGCTTCTCC ACCTCTTCTG CCAGCAGCGC
    AGACTCAAGG CTCCTGTGTA CCTCACGGAC CACGTGGTGT TCCAGGATAA AGAATACAGC
    ATTGAAGAAA TAGAGGCTGG CCGAGTCCCG AACCCACACC TGGGCCCAGT GGAAGAGCGT
    CTGGCCTTGC ATGTCCTCCA GCAGCAGGGC CTGGTCCCTG AGCATGTGGA GTCCCGGCCT
    CTCTACAGCC CCCTGCAGCC AGACATTGAA CAGGGGAAGC TGCAGATGTG GATCGACCTA
    TTTCCCAAGG CCCTGGGGCG TCCCGGACCT CCCTTCAACA TCACCCCGCG GAGAGCCAGA
    AGGTTTTTCC TGCGTTGTAT CATCTGGAAC ACAAAGGACG TGATCCTGGA CGACCTCAGC
    ATCACAGGGG AGAAGATGAG TGATATTTAT GTGAAGGGTT GGATGGTTGG CTTTGAAGAG
    CACAAGCAAA AGACAGATGT GCATTACCGG TCCCTGGGAG GTGAAGGCAA CTTTAACTGG
    AGGTTCATTT TCCCCTTTGA CTACCTGCCA GCCGAGCAAG TCTGCACCGT CTCTAAGAAG
    GATGCTTTCT GGAGGCTGGA CAAGACTGAG AGCAAAATCC CAGCACGAGT GGTATTTCAG
    ATCTGGGACA ATGACAAGTT CTCCTTTGAC GACTTTCTGG GCTCCTTGCA GTTGGATCTC
    AACCACATGC CCAAGCCTGC CAAGACAGCT GAGAAGTGCT CCTTGGACCA GCTGGATGAC
    ACTTTCCACC CGGAGTGGTT TGTGTCCCTT TTTGAACAGA AAACAGTTAA GGGCTGGTGG
    CCCTGCGTAG CAGAAGAGGG CGAGAAGAAG ATCCTGGCAG GCAAGCTGGA AATGACCTTG
    GAGATCGTAG CAGAGAGTGA GCATGAGGAG CGGCCTGCTG GCCAGGGCCG GGATGAACCC
    AACATGAACC CCAAGCTCGA GGACCCAAGG CGCCCTGACA CCTCCTTCCT CTGGTTCACC
    TCTCCATACA AGACTATGAA GTTCATCCTG TGGCGGCGGT TCCGATGTGC CATTATCCTC
    TTCATCATTC TCTTCATCCT GCTACTATTC TTAGGCATCT TCATCTATGC TTTCCCGAAC
    TACGCTGCCA TGAAGCTGGT GAAGCCCTTC AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe19214
    Clone ID Related Accession (Same CDS sequence) XM_003432231.4 , XM_003432231.3
    Accession Version XM_003432231.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6276bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product dysferlin isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003432231.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG ACACAGGAGG AGAGGAGGAC
    ACTGAGGACC AGGGGCTCAC TGGAGACGAG GGAGAGCCAT TACTGGATCA GAGTGGCGTC
    CCAGGCCCTG GGGCTCCCAC CACCCCGAGG AAGCCACCTT CCCATCTGCC CCCCTACCAC
    CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC GCAGCCAGAA AGCTGCTGTC CGACAAACCG
    CAGGACTTCC AGATCAGAGT GCAAGTGATC GAGGGCCGCC AGCTGCCAGG GGTGAATATC
    AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG CAGACCAAGA GGACACGGAT CCACAAGGGA
    AACAGCCCAC TATTTAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TTCGGAGCTG
    TTTGATGAGC CCATCTTTAT CACGGTCGTA GACTCCCGTT CTCTCAGGAC AGATGCTCTC
    ATTGGGGAAT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA TGCTTATCTG
    AGGAAGTGGC TGCTACTCTC CAACCCTGAT GACTTCTCTG TTGGGGCCAG AGGTTACCTG
    AAAGCAAGCC TTTATGTGCT GGGGCCCGGA GATGAAGCGC CTCTGGAGAG AAAGGACCCC
    TCTGAAGACA AGGAGGACAT TGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTGCGA
    GGAGCCCACT TCTGCCTGAA GATCTTCAGA GCGGAGGACT TACCACAAAT GGATGATGCC
    GTGATGGACA ATGTGAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA TTTGGTAGAT
    CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA
    GCCAACCCTC AGTGGAACCA GAGCATCACT CTGCCTGCCA TGTTTCCCTC CATGTGTGAA
    AAAATGAAGA TTCGCGTCAT GGACTGGGAC CGCCTTACCC ACAATGACAT CGTGGCCACC
    ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGAAGT GGATGACCAT
    CTGGGCTTCC TTCCCACCTT CGGGCCCTGC TTCGTCAATC TCTATGGCAG CCCCAGGGAA
    TTCACTGGCT TCCCGGACCC CTACGCGGAA CTCAACATGG GCAAGGGGGA AGGCGTGGCC
    TACCGAGGTC GGCTTCTGCT CTCCCTGGAG ACCAAGCTGG TGGAGCACAG CGGCGAGCAA
    AGGGTGGAGG ACCTCCCTGC TGATGACATC CTTCGGGTGG AGAAATACCT CCGGAGGCGC
    AAGTACTCTC TCTTTGCCGC CTTCTACTCG GCCACCATGC TCCAGGATGT CGACGATGCC
    ATCCAGTTTG AGGTCAGCAT TGGGAACTAT GGCAACAAGT TCGACACGAC CTGTCTGCCA
    CTGGCCTCCA CCACCCAGTA CAGCCGGGCA GTCTTTGATG GGTGCCACTA CTACTACTTG
    CCCTGGGGCA ATGTGAAGCC TGTGGTGGTG CTGTCATCCT ACTGGGAAGA CATCAGCCAC
    AGAGTCGAGG CTCAGAACCA GCTGCTCAGG ATCGCTGACC ATCTGGAAGC TGGCCTGGAG
    CAAGTCCACT TGGCTTTAAA GGCGCAGTTC AACACAGAGG ATGTGGACTC CCTGTTGGCC
    CAGCTGATGG ATGAGCTCAT CTCAGACTGC AGCCAGCCCC TGGGCAATGT CCAAGAGATA
    CCCTCTGCCA CCCACCTGGA CCGGCACCTG TACAGGCTGC GCACCCGTCA CCTAGGCCAG
    ATTACTGAGG CGGCCCTGGC TCTGAAACTC GGGCATGGCA AGCTCCCTGC CGCCCTGGAG
    CAGGCTGAAG ACTGGCTCCT GCGTCTCCGT GCCCTGGCTG ATGAGCCCCA GAACAGCCTG
    CCCGACATCA TCATCTGGAT GCTGCAGGGA AACAAGCGAG TGGCGTACCA GCGGGTGCCT
    GCGCACGAGG TCCTCTTCTC CCGGCGGGGC GCCAGCTACT GTGGCAAGAA CTGTGGGAAG
    CTGCAGACGA TATTTCTGAA ATATCCCATG GAGGGGGTGC CCGGAGCCCG GATGCCAGTA
    CAAATACGGG TGAAACTCTG GTTTGGGCTT TCTGTGGATG AGAAGGAGTT CAACCAGTTT
    GCTGAGGGAA AGCTGTCCGT CTTTGCTGAA ACTTATGAGA ACCAGACCAA ACTGGCCCTG
    GTTGGGAACT GGGGCACAAC AGGCCTCACC TACCCCAAGT TTTCTGATGT CATGGGCAAG
    ATCAAGCTAC CCAAGGATAG CTTCCGCCCC TCAGCTGGCT GGACCTGGGC CGGAGACTGG
    TTCGTGTGTC CAGAGAAGAC CCTACTCCAT GACATGGACG CGGGCCACCT GAGTTTTGTG
    GAGGAGGTGT TTGAGAACCA GACCCGGCTC CCTGGAGGCC AGTGGATCTA CATGAGTGAC
    AACTACACAG ATGTGAATGG GGATAAGGTG CTTCCTAAGG ATGACATTGA GTGCCCACTG
    GGCTGGAAGT GGGAAGATGA AGAGTGGTCC ACGGACCTCA ATCGGGCCGT CGATGAGCAA
    GGCTGGGAGT ACAGTATCAC CATCCCCCCG GATCGGAAGC CAAAGCACTG GGTTCCTGCT
    GAGAAGATGT ATTACACGCA CCGTCGGCGG CGCTGGGTCC GCCTGCGCAG AAGGGACCTC
    AGCCAGATGG AGGCATTGAA GAGGCACAGA CAGGCAGAGG CAGAGGGTGA GGGCTGGGAG
    TACGCCTCGC TCTTGGGCTG GAAGTTCCAC CTGGAGTACC GCAAGACTGA TGCTTTCCGC
    CGCCGCCGCT GGCGCCGCCG CATGGAACCG CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT
    GCCCTTGAGG GGGCTCTGGG TGGTGTGGTA GATGACAGGA GTGAAGATTC CGTGTCCGTG
    TCCACCTTGA GCTTTGGAGT GAACAGACCC ACGATTTCCT GCATCTTTGA TTATGGGAAC
    CGCTACCACC TACGCTGCTA CATGTACCAG GCTCGGGACC TGCCCTCGAT GGACAAGGAC
    TCTTTTTCTG ATCCCTACGC CATCGTCTCC TTCCTGCACC AGAGCCAGAA GACAGTGGTG
    GCGAAGAACA CTCTGAACCC CACCTGGGAC CAGACGCTTA TCTTCTATGA GATTGAGATC
    TTTGGTGAGC CAGCCAGTAT CGCTGAGCAG CCGCCCAGCA TTGTGGTGGA GCTGTATGAC
    CACGACACCT ACGGTGCAGA TGAGTTTATG GGACGCTGCA TTTGTCAGCC CAGCCTGGAG
    CGGACACCCC GGCTGGCCTG GTTCCCACTG ATTAGGGGCA GCCAGTCGGC GGGGGAACTG
    CTGGCCTCTT TTGAGCTCAT CCAGAGAGAA AAGCCGGCCA TTCACCATAT TCCTGGTTTT
    GAGGTACAGG ATATGTCAGG AATCCTGGAA GAGTCAGAGG ACATAGACCT GCCCTACCCG
    CCACCTCAGA GGGAGGCCAA CATCTTCATG GTCCCCCAGA ACATCAAGCC TGTTCTCCAG
    CGTACTGCCA TAGAGATCCT GGCGTGGGGC CTGCGGAACA TGAAGAGCTA CCAGCTAGCC
    AGCATCTCCT CCCCTAGCCT TGTGGTAGAG TGCGGGGGCC AGACAGTGCA GTCCTGTGTC
    ATCAAGAACC TCCGGAAGAA CCCCAACTTC GACATCTGTA CCCTTTTCAT GGAAGTGATG
    CTGCCCAAGG AGGAGGTCTA CTGTCCCTCC ATCGTGATCA AGGTCATTGA TAACCGCCAG
    TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT ACCATCCGTT CCCTGGAGAG CTTCATGTGT
    GACCTGTACT CCGAGGAGAA TCCGTCCCCA CAGGGCGGCC CAGATGATGT GAGTTTACTC
    AGTCCTGGGG AAGACGTGCT CATTGACATC GATGACAAGG AGCCCCTCAT CCCCATGCAG
    CTTGCAGATG GTCTGTCGGG CTCTGCCCCC ACTAACATGG CATCTTCTCC ATCCAGTCCT
    CATGAGGAAG AGTTCATCGA CTGGTGGAGC AAATTCTTTG CCTCCACAGG GGAGAGGGAA
    AAGTGCGGCT CATACTTGGA GAAGGGCTTT GACACTCTAA AGGTCTATGA CACACAACTG
    GAGAACGTGG AGGCCTTTGA GGGCCTGTCT GACTTTTGTA ACACCTTCAA GCTCTACCGG
    GGCAAGACTC AGGAGGAGAC AGAGGACCCA TCTGTTATTG GGGAGTTTAA GGGCCTCTTC
    AAAATCTATC CCCTCCCAGA AGACCCGGCT GTTCCCATGC CCCCGAGACA ATTCCACCAG
    CTGGCTGCCC AAGGGCCTCA GGAGTGCCTG GTCCGCATCT ATATCATCCG AGCATTTGGC
    CTTCAGCCCA AGGACCCCAA TGGGAAGTGT GATCCTTACA TCAAGATCTC CATAGGGAAG
    AAATCAGTGA GTGACCAGGA TAACTACATT CCCTGTACTC TGGAGCCTGT GTTTGGAAAG
    ATGTTCGAGC TGACCTGCAC TCTGCCTCTG GAGAAGGACC TGAAGATCAC ACTCTATGAC
    TATGACCTCC TCTCCAAAGA TGAAAAGATT GGGGAGACAG TCATTGACCT GGAGGATAGG
    CTGCTGTCCA AGTTTGGGGC TCGCTGCGGA CTCCCACAGA CGTACTGTGT CTCTGGACCC
    AACCAGTGGA GGGACCAGCT CCGCCCCTCC CAGCTTCTCC ACCTCTTCTG CCAGCAGCGC
    AGACTCAAGG CTCCTGTGTA CCTCACGGAC CACGTGGTGT TCCAGGATAA AGAATACAGC
    ATTGAAGAAA TAGAGGCTGG CCGAGTCCCG AACCCACACC TGGGCCCAGT GGAAGAGCGT
    CTGGCCTTGC ATGTCCTCCA GCAGCAGGGC CTGGTCCCTG AGCATGTGGA GTCCCGGCCT
    CTCTACAGCC CCCTGCAGCC AGACATTGAA CAGGGGAAGC TGCAGATGTG GATCGACCTA
    TTTCCCAAGG CCCTGGGGCG TCCCGGACCT CCCTTCAACA TCACCCCGCG GAGAGCCAGA
    AGGTTTTTCC TGCGTTGTAT CATCTGGAAC ACAAAGGACG TGATCCTGGA CGACCTCAGC
    ATCACAGGGG AGAAGATGAG TGATATTTAT GTGAAGGGTT GGATGGTTGG CTTTGAAGAG
    CACAAGCAAA AGACAGATGT GCATTACCGG TCCCTGGGAG GTGAAGGCAA CTTTAACTGG
    AGGTTCATTT TCCCCTTTGA CTACCTGCCA GCCGAGCAAG TCTGCACCGT CTCTAAGAAG
    GATGCTTTCT GGAGGCTGGA CAAGACTGAG AGCAAAATCC CAGCACGAGT GGTATTTCAG
    ATCTGGGACA ATGACAAGTT CTCCTTTGAC GACTTTCTGG GCTCCTTGCA GTTGGATCTC
    AACCACATGC CCAAGCCTGC CAAGACAGCT GAGAAGTGCT CCTTGGACCA GCTGGATGAC
    ACTTTCCACC CGGAGTGGTT TGTGTCCCTT TTTGAACAGA AAACAGTTAA GGGCTGGTGG
    CCCTGCGTAG CAGAAGAGGG CGAGAAGAAG ATCCTGGCAG GCAAGCTGGA AATGACCTTG
    GAGATCGTAG CAGAGAGTGA GCATGAGGAG CGGCCTGCTG GCCAGGGCCG GGATGAACCC
    AACATGAACC CCAAGCTCGA GGACCCAAGG CGCCCTGACA CCTCCTTCCT CTGGTTCACC
    TCTCCATACA AGACTATGAA GTTCATCCTG TGGCGGCGGT TCCGATGTGC CATTATCCTC
    TTCATCATTC TCTTCATCCT GCTACTATTC TTAGGCATCT TCATCTATGC TTTCCCGAAC
    TACGCTGCCA TGAAGCTGGT GAAGCCCTTC AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003432279.1
    CDS51..6326
    Translation

    Target ORF information:

    RefSeq Version XM_003432231.3
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris dysferlin (DYSF), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003432231.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG ACACAGGAGG AGAGGAGGAC
    ACTGAGGACC AGGGGCTCAC TGGAGACGAG GGAGAGCCAT TACTGGATCA GAGTGGCGTC
    CCAGGCCCTG GGGCTCCCAC CACCCCGAGG AAGCCACCTT CCCATCTGCC CCCCTACCAC
    CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC GCAGCCAGAA AGCTGCTGTC CGACAAACCG
    CAGGACTTCC AGATCAGAGT GCAAGTGATC GAGGGCCGCC AGCTGCCAGG GGTGAATATC
    AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG CAGACCAAGA GGACACGGAT CCACAAGGGA
    AACAGCCCAC TATTTAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TTCGGAGCTG
    TTTGATGAGC CCATCTTTAT CACGGTCGTA GACTCCCGTT CTCTCAGGAC AGATGCTCTC
    ATTGGGGAAT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA TGCTTATCTG
    AGGAAGTGGC TGCTACTCTC CAACCCTGAT GACTTCTCTG TTGGGGCCAG AGGTTACCTG
    AAAGCAAGCC TTTATGTGCT GGGGCCCGGA GATGAAGCGC CTCTGGAGAG AAAGGACCCC
    TCTGAAGACA AGGAGGACAT TGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTGCGA
    GGAGCCCACT TCTGCCTGAA GATCTTCAGA GCGGAGGACT TACCACAAAT GGATGATGCC
    GTGATGGACA ATGTGAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA TTTGGTAGAT
    CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA
    GCCAACCCTC AGTGGAACCA GAGCATCACT CTGCCTGCCA TGTTTCCCTC CATGTGTGAA
    AAAATGAAGA TTCGCGTCAT GGACTGGGAC CGCCTTACCC ACAATGACAT CGTGGCCACC
    ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGAAGT GGATGACCAT
    CTGGGCTTCC TTCCCACCTT CGGGCCCTGC TTCGTCAATC TCTATGGCAG CCCCAGGGAA
    TTCACTGGCT TCCCGGACCC CTACGCGGAA CTCAACATGG GCAAGGGGGA AGGCGTGGCC
    TACCGAGGTC GGCTTCTGCT CTCCCTGGAG ACCAAGCTGG TGGAGCACAG CGGCGAGCAA
    AGGGTGGAGG ACCTCCCTGC TGATGACATC CTTCGGGTGG AGAAATACCT CCGGAGGCGC
    AAGTACTCTC TCTTTGCCGC CTTCTACTCG GCCACCATGC TCCAGGATGT CGACGATGCC
    ATCCAGTTTG AGGTCAGCAT TGGGAACTAT GGCAACAAGT TCGACACGAC CTGTCTGCCA
    CTGGCCTCCA CCACCCAGTA CAGCCGGGCA GTCTTTGATG GGTGCCACTA CTACTACTTG
    CCCTGGGGCA ATGTGAAGCC TGTGGTGGTG CTGTCATCCT ACTGGGAAGA CATCAGCCAC
    AGAGTCGAGG CTCAGAACCA GCTGCTCAGG ATCGCTGACC ATCTGGAAGC TGGCCTGGAG
    CAAGTCCACT TGGCTTTAAA GGCGCAGTTC AACACAGAGG ATGTGGACTC CCTGTTGGCC
    CAGCTGATGG ATGAGCTCAT CTCAGACTGC AGCCAGCCCC TGGGCAATGT CCAAGAGATA
    CCCTCTGCCA CCCACCTGGA CCGGCACCTG TACAGGCTGC GCACCCGTCA CCTAGGCCAG
    ATTACTGAGG CGGCCCTGGC TCTGAAACTC GGGCATGGCA AGCTCCCTGC CGCCCTGGAG
    CAGGCTGAAG ACTGGCTCCT GCGTCTCCGT GCCCTGGCTG ATGAGCCCCA GAACAGCCTG
    CCCGACATCA TCATCTGGAT GCTGCAGGGA AACAAGCGAG TGGCGTACCA GCGGGTGCCT
    GCGCACGAGG TCCTCTTCTC CCGGCGGGGC GCCAGCTACT GTGGCAAGAA CTGTGGGAAG
    CTGCAGACGA TATTTCTGAA ATATCCCATG GAGGGGGTGC CCGGAGCCCG GATGCCAGTA
    CAAATACGGG TGAAACTCTG GTTTGGGCTT TCTGTGGATG AGAAGGAGTT CAACCAGTTT
    GCTGAGGGAA AGCTGTCCGT CTTTGCTGAA ACTTATGAGA ACCAGACCAA ACTGGCCCTG
    GTTGGGAACT GGGGCACAAC AGGCCTCACC TACCCCAAGT TTTCTGATGT CATGGGCAAG
    ATCAAGCTAC CCAAGGATAG CTTCCGCCCC TCAGCTGGCT GGACCTGGGC CGGAGACTGG
    TTCGTGTGTC CAGAGAAGAC CCTACTCCAT GACATGGACG CGGGCCACCT GAGTTTTGTG
    GAGGAGGTGT TTGAGAACCA GACCCGGCTC CCTGGAGGCC AGTGGATCTA CATGAGTGAC
    AACTACACAG ATGTGAATGG GGATAAGGTG CTTCCTAAGG ATGACATTGA GTGCCCACTG
    GGCTGGAAGT GGGAAGATGA AGAGTGGTCC ACGGACCTCA ATCGGGCCGT CGATGAGCAA
    GGCTGGGAGT ACAGTATCAC CATCCCCCCG GATCGGAAGC CAAAGCACTG GGTTCCTGCT
    GAGAAGATGT ATTACACGCA CCGTCGGCGG CGCTGGGTCC GCCTGCGCAG AAGGGACCTC
    AGCCAGATGG AGGCATTGAA GAGGCACAGA CAGGCAGAGG CAGAGGGTGA GGGCTGGGAG
    TACGCCTCGC TCTTGGGCTG GAAGTTCCAC CTGGAGTACC GCAAGACTGA TGCTTTCCGC
    CGCCGCCGCT GGCGCCGCCG CATGGAACCG CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT
    GCCCTTGAGG GGGCTCTGGG TGGTGTGGTA GATGACAGGA GTGAAGATTC CGTGTCCGTG
    TCCACCTTGA GCTTTGGAGT GAACAGACCC ACGATTTCCT GCATCTTTGA TTATGGGAAC
    CGCTACCACC TACGCTGCTA CATGTACCAG GCTCGGGACC TGCCCTCGAT GGACAAGGAC
    TCTTTTTCTG ATCCCTACGC CATCGTCTCC TTCCTGCACC AGAGCCAGAA GACAGTGGTG
    GCGAAGAACA CTCTGAACCC CACCTGGGAC CAGACGCTTA TCTTCTATGA GATTGAGATC
    TTTGGTGAGC CAGCCAGTAT CGCTGAGCAG CCGCCCAGCA TTGTGGTGGA GCTGTATGAC
    CACGACACCT ACGGTGCAGA TGAGTTTATG GGACGCTGCA TTTGTCAGCC CAGCCTGGAG
    CGGACACCCC GGCTGGCCTG GTTCCCACTG ATTAGGGGCA GCCAGTCGGC GGGGGAACTG
    CTGGCCTCTT TTGAGCTCAT CCAGAGAGAA AAGCCGGCCA TTCACCATAT TCCTGGTTTT
    GAGGTACAGG ATATGTCAGG AATCCTGGAA GAGTCAGAGG ACATAGACCT GCCCTACCCG
    CCACCTCAGA GGGAGGCCAA CATCTTCATG GTCCCCCAGA ACATCAAGCC TGTTCTCCAG
    CGTACTGCCA TAGAGATCCT GGCGTGGGGC CTGCGGAACA TGAAGAGCTA CCAGCTAGCC
    AGCATCTCCT CCCCTAGCCT TGTGGTAGAG TGCGGGGGCC AGACAGTGCA GTCCTGTGTC
    ATCAAGAACC TCCGGAAGAA CCCCAACTTC GACATCTGTA CCCTTTTCAT GGAAGTGATG
    CTGCCCAAGG AGGAGGTCTA CTGTCCCTCC ATCGTGATCA AGGTCATTGA TAACCGCCAG
    TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT ACCATCCGTT CCCTGGAGAG CTTCATGTGT
    GACCTGTACT CCGAGGAGAA TCCGTCCCCA CAGGGCGGCC CAGATGATGT GAGTTTACTC
    AGTCCTGGGG AAGACGTGCT CATTGACATC GATGACAAGG AGCCCCTCAT CCCCATGCAG
    CTTGCAGATG GTCTGTCGGG CTCTGCCCCC ACTAACATGG CATCTTCTCC ATCCAGTCCT
    CATGAGGAAG AGTTCATCGA CTGGTGGAGC AAATTCTTTG CCTCCACAGG GGAGAGGGAA
    AAGTGCGGCT CATACTTGGA GAAGGGCTTT GACACTCTAA AGGTCTATGA CACACAACTG
    GAGAACGTGG AGGCCTTTGA GGGCCTGTCT GACTTTTGTA ACACCTTCAA GCTCTACCGG
    GGCAAGACTC AGGAGGAGAC AGAGGACCCA TCTGTTATTG GGGAGTTTAA GGGCCTCTTC
    AAAATCTATC CCCTCCCAGA AGACCCGGCT GTTCCCATGC CCCCGAGACA ATTCCACCAG
    CTGGCTGCCC AAGGGCCTCA GGAGTGCCTG GTCCGCATCT ATATCATCCG AGCATTTGGC
    CTTCAGCCCA AGGACCCCAA TGGGAAGTGT GATCCTTACA TCAAGATCTC CATAGGGAAG
    AAATCAGTGA GTGACCAGGA TAACTACATT CCCTGTACTC TGGAGCCTGT GTTTGGAAAG
    ATGTTCGAGC TGACCTGCAC TCTGCCTCTG GAGAAGGACC TGAAGATCAC ACTCTATGAC
    TATGACCTCC TCTCCAAAGA TGAAAAGATT GGGGAGACAG TCATTGACCT GGAGGATAGG
    CTGCTGTCCA AGTTTGGGGC TCGCTGCGGA CTCCCACAGA CGTACTGTGT CTCTGGACCC
    AACCAGTGGA GGGACCAGCT CCGCCCCTCC CAGCTTCTCC ACCTCTTCTG CCAGCAGCGC
    AGACTCAAGG CTCCTGTGTA CCTCACGGAC CACGTGGTGT TCCAGGATAA AGAATACAGC
    ATTGAAGAAA TAGAGGCTGG CCGAGTCCCG AACCCACACC TGGGCCCAGT GGAAGAGCGT
    CTGGCCTTGC ATGTCCTCCA GCAGCAGGGC CTGGTCCCTG AGCATGTGGA GTCCCGGCCT
    CTCTACAGCC CCCTGCAGCC AGACATTGAA CAGGGGAAGC TGCAGATGTG GATCGACCTA
    TTTCCCAAGG CCCTGGGGCG TCCCGGACCT CCCTTCAACA TCACCCCGCG GAGAGCCAGA
    AGGTTTTTCC TGCGTTGTAT CATCTGGAAC ACAAAGGACG TGATCCTGGA CGACCTCAGC
    ATCACAGGGG AGAAGATGAG TGATATTTAT GTGAAGGGTT GGATGGTTGG CTTTGAAGAG
    CACAAGCAAA AGACAGATGT GCATTACCGG TCCCTGGGAG GTGAAGGCAA CTTTAACTGG
    AGGTTCATTT TCCCCTTTGA CTACCTGCCA GCCGAGCAAG TCTGCACCGT CTCTAAGAAG
    GATGCTTTCT GGAGGCTGGA CAAGACTGAG AGCAAAATCC CAGCACGAGT GGTATTTCAG
    ATCTGGGACA ATGACAAGTT CTCCTTTGAC GACTTTCTGG GCTCCTTGCA GTTGGATCTC
    AACCACATGC CCAAGCCTGC CAAGACAGCT GAGAAGTGCT CCTTGGACCA GCTGGATGAC
    ACTTTCCACC CGGAGTGGTT TGTGTCCCTT TTTGAACAGA AAACAGTTAA GGGCTGGTGG
    CCCTGCGTAG CAGAAGAGGG CGAGAAGAAG ATCCTGGCAG GCAAGCTGGA AATGACCTTG
    GAGATCGTAG CAGAGAGTGA GCATGAGGAG CGGCCTGCTG GCCAGGGCCG GGATGAACCC
    AACATGAACC CCAAGCTCGA GGACCCAAGG CGCCCTGACA CCTCCTTCCT CTGGTTCACC
    TCTCCATACA AGACTATGAA GTTCATCCTG TGGCGGCGGT TCCGATGTGC CATTATCCTC
    TTCATCATTC TCTTCATCCT GCTACTATTC TTAGGCATCT TCATCTATGC TTTCCCGAAC
    TACGCTGCCA TGAAGCTGGT GAAGCCCTTC AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe19215
    Clone ID Related Accession (Same CDS sequence) XM_003432224.4 , XM_003432224.3
    Accession Version XM_003432224.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6273bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product dysferlin isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003432224.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC
    CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC
    TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA
    CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGACA CAGGAGGAGA GGAGGACACT
    GAGGACCAGG GGCTCACTGG AGACGAGGGA GAGCCATTAC TGGATCAGAG TGGCGTCCCA
    GGCCCTGGGG CTCCCACCAC CCCGAGGAAG CCACCTTCCC ATCTGCCCCC CTACCACCCT
    GGGAGCAAGA GGAAAAGAAG TGCTCCCGCA GCCAGAAAGC TGCTGTCCGA CAAACCGCAG
    GACTTCCAGA TCAGAGTGCA AGTGATCGAG GGCCGCCAGC TGCCAGGGGT GAATATCAAG
    CCTGTGATCA AGGTCACTGC TGCTGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC
    AGCCCACTAT TTAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTTC GGAGCTGTTT
    GATGAGCCCA TCTTTATCAC GGTCGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT
    GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACATGC TTATCTGAGG
    AAGTGGCTGC TACTCTCCAA CCCTGATGAC TTCTCTGTTG GGGCCAGAGG TTACCTGAAA
    GCAAGCCTTT ATGTGCTGGG GCCCGGAGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT
    GAAGACAAGG AGGACATTGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTGCGAGGA
    GCCCACTTCT GCCTGAAGAT CTTCAGAGCG GAGGACTTAC CACAAATGGA TGATGCCGTG
    ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAATTT GGTAGATCCC
    TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC
    AACCCTCAGT GGAACCAGAG CATCACTCTG CCTGCCATGT TTCCCTCCAT GTGTGAAAAA
    ATGAAGATTC GCGTCATGGA CTGGGACCGC CTTACCCACA ATGACATCGT GGCCACCACC
    TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGTGGA TGACCATCTG
    GGCTTCCTTC CCACCTTCGG GCCCTGCTTC GTCAATCTCT ATGGCAGCCC CAGGGAATTC
    ACTGGCTTCC CGGACCCCTA CGCGGAACTC AACATGGGCA AGGGGGAAGG CGTGGCCTAC
    CGAGGTCGGC TTCTGCTCTC CCTGGAGACC AAGCTGGTGG AGCACAGCGG CGAGCAAAGG
    GTGGAGGACC TCCCTGCTGA TGACATCCTT CGGGTGGAGA AATACCTCCG GAGGCGCAAG
    TACTCTCTCT TTGCCGCCTT CTACTCGGCC ACCATGCTCC AGGATGTCGA CGATGCCATC
    CAGTTTGAGG TCAGCATTGG GAACTATGGC AACAAGTTCG ACACGACCTG TCTGCCACTG
    GCCTCCACCA CCCAGTACAG CCGGGCAGTC TTTGATGGGT GCCACTACTA CTACTTGCCC
    TGGGGCAATG TGAAGCCTGT GGTGGTGCTG TCATCCTACT GGGAAGACAT CAGCCACAGA
    GTCGAGGCTC AGAACCAGCT GCTCAGGATC GCTGACCATC TGGAAGCTGG CCTGGAGCAA
    GTCCACTTGG CTTTAAAGGC GCAGTTCAAC ACAGAGGATG TGGACTCCCT GTTGGCCCAG
    CTGATGGATG AGCTCATCTC AGACTGCAGC CAGCCCCTGG GCAATGTCCA AGAGATACCC
    TCTGCCACCC ACCTGGACCG GCACCTGTAC AGGCTGCGCA CCCGTCACCT AGGCCAGATT
    ACTGAGGCGG CCCTGGCTCT GAAACTCGGG CATGGCAAGC TCCCTGCCGC CCTGGAGCAG
    GCTGAAGACT GGCTCCTGCG TCTCCGTGCC CTGGCTGATG AGCCCCAGAA CAGCCTGCCC
    GACATCATCA TCTGGATGCT GCAGGGAAAC AAGCGAGTGG CGTACCAGCG GGTGCCTGCG
    CACGAGGTCC TCTTCTCCCG GCGGGGCGCC AGCTACTGTG GCAAGAACTG TGGGAAGCTG
    CAGACGATAT TTCTGAAATA TCCCATGGAG GGGGTGCCCG GAGCCCGGAT GCCAGTACAA
    ATACGGGTGA AACTCTGGTT TGGGCTTTCT GTGGATGAGA AGGAGTTCAA CCAGTTTGCT
    GAGGGAAAGC TGTCCGTCTT TGCTGAAACT TATGAGAACC AGACCAAACT GGCCCTGGTT
    GGGAACTGGG GCACAACAGG CCTCACCTAC CCCAAGTTTT CTGATGTCAT GGGCAAGATC
    AAGCTACCCA AGGATAGCTT CCGCCCCTCA GCTGGCTGGA CCTGGGCCGG AGACTGGTTC
    GTGTGTCCAG AGAAGACCCT ACTCCATGAC ATGGACGCGG GCCACCTGAG TTTTGTGGAG
    GAGGTGTTTG AGAACCAGAC CCGGCTCCCT GGAGGCCAGT GGATCTACAT GAGTGACAAC
    TACACAGATG TGAATGGGGA TAAGGTGCTT CCTAAGGATG ACATTGAGTG CCCACTGGGC
    TGGAAGTGGG AAGATGAAGA GTGGTCCACG GACCTCAATC GGGCCGTCGA TGAGCAAGGC
    TGGGAGTACA GTATCACCAT CCCCCCGGAT CGGAAGCCAA AGCACTGGGT TCCTGCTGAG
    AAGATGTATT ACACGCACCG TCGGCGGCGC TGGGTCCGCC TGCGCAGAAG GGACCTCAGC
    CAGATGGAGG CATTGAAGAG GCACAGACAG GCAGAGGCAG AGGGTGAGGG CTGGGAGTAC
    GCCTCGCTCT TGGGCTGGAA GTTCCACCTG GAGTACCGCA AGACTGATGC TTTCCGCCGC
    CGCCGCTGGC GCCGCCGCAT GGAACCGCTG GAGAAGACGG GGCCTGCAGC CGTGTTTGCC
    CTTGAGGGGG CTCTGGGTGG TGTGGTAGAT GACAGGAGTG AAGATTCCGT GTCCGTGTCC
    ACCTTGAGCT TTGGAGTGAA CAGACCCACG ATTTCCTGCA TCTTTGATTA TGGGAACCGC
    TACCACCTAC GCTGCTACAT GTACCAGGCT CGGGACCTGC CCTCGATGGA CAAGGACTCT
    TTTTCTGATC CCTACGCCAT CGTCTCCTTC CTGCACCAGA GCCAGAAGAC AGTGGTGGCG
    AAGAACACTC TGAACCCCAC CTGGGACCAG ACGCTTATCT TCTATGAGAT TGAGATCTTT
    GGTGAGCCAG CCAGTATCGC TGAGCAGCCG CCCAGCATTG TGGTGGAGCT GTATGACCAC
    GACACCTACG GTGCAGATGA GTTTATGGGA CGCTGCATTT GTCAGCCCAG CCTGGAGCGG
    ACACCCCGGC TGGCCTGGTT CCCACTGATT AGGGGCAGCC AGTCGGCGGG GGAACTGCTG
    GCCTCTTTTG AGCTCATCCA GAGAGAAAAG CCGGCCATTC ACCATATTCC TGGTTTTGAG
    GTACAGGATA TGTCAGGAAT CCTGGAAGAG TCAGAGGACA TAGACCTGCC CTACCCGCCA
    CCTCAGAGGG AGGCCAACAT CTTCATGGTC CCCCAGAACA TCAAGCCTGT TCTCCAGCGT
    ACTGCCATAG AGATCCTGGC GTGGGGCCTG CGGAACATGA AGAGCTACCA GCTAGCCAGC
    ATCTCCTCCC CTAGCCTTGT GGTAGAGTGC GGGGGCCAGA CAGTGCAGTC CTGTGTCATC
    AAGAACCTCC GGAAGAACCC CAACTTCGAC ATCTGTACCC TTTTCATGGA AGTGATGCTG
    CCCAAGGAGG AGGTCTACTG TCCCTCCATC GTGATCAAGG TCATTGATAA CCGCCAGTTT
    GGCCGCCGGC CTGTGGTGGG CCAGTGTACC ATCCGTTCCC TGGAGAGCTT CATGTGTGAC
    CTGTACTCCG AGGAGAATCC GTCCCCACAG GGCGGCCCAG ATGATGTGAG TTTACTCAGT
    CCTGGGGAAG ACGTGCTCAT TGACATCGAT GACAAGGAGC CCCTCATCCC CATGCAGCTT
    GCAGATGGTC TGTCGGGCTC TGCCCCCACT AACATGGCAT CTTCTCCATC CAGTCCTCAT
    GAGGAAGAGT TCATCGACTG GTGGAGCAAA TTCTTTGCCT CCACAGGGGA GAGGGAAAAG
    TGCGGCTCAT ACTTGGAGAA GGGCTTTGAC ACTCTAAAGG TCTATGACAC ACAACTGGAG
    AACGTGGAGG CCTTTGAGGG CCTGTCTGAC TTTTGTAACA CCTTCAAGCT CTACCGGGGC
    AAGACTCAGG AGGAGACAGA GGACCCATCT GTTATTGGGG AGTTTAAGGG CCTCTTCAAA
    ATCTATCCCC TCCCAGAAGA CCCGGCTGTT CCCATGCCCC CGAGACAATT CCACCAGCTG
    GCTGCCCAAG GGCCTCAGGA GTGCCTGGTC CGCATCTATA TCATCCGAGC ATTTGGCCTT
    CAGCCCAAGG ACCCCAATGG GAAGTGTGAT CCTTACATCA AGATCTCCAT AGGGAAGAAA
    TCAGTGAGTG ACCAGGATAA CTACATTCCC TGTACTCTGG AGCCTGTGTT TGGAAAGATG
    TTCGAGCTGA CCTGCACTCT GCCTCTGGAG AAGGACCTGA AGATCACACT CTATGACTAT
    GACCTCCTCT CCAAAGATGA AAAGATTGGG GAGACAGTCA TTGACCTGGA GGATAGGCTG
    CTGTCCAAGT TTGGGGCTCG CTGCGGACTC CCACAGACGT ACTGTGTCTC TGGACCCAAC
    CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG CTTCTCCACC TCTTCTGCCA GCAGCGCAGA
    CTCAAGGCTC CTGTGTACCT CACGGACCAC GTGGTGTTCC AGGATAAAGA ATACAGCATT
    GAAGAAATAG AGGCTGGCCG AGTCCCGAAC CCACACCTGG GCCCAGTGGA AGAGCGTCTG
    GCCTTGCATG TCCTCCAGCA GCAGGGCCTG GTCCCTGAGC ATGTGGAGTC CCGGCCTCTC
    TACAGCCCCC TGCAGCCAGA CATTGAACAG GGGAAGCTGC AGATGTGGAT CGACCTATTT
    CCCAAGGCCC TGGGGCGTCC CGGACCTCCC TTCAACATCA CCCCGCGGAG AGCCAGAAGG
    TTTTTCCTGC GTTGTATCAT CTGGAACACA AAGGACGTGA TCCTGGACGA CCTCAGCATC
    ACAGGGGAGA AGATGAGTGA TATTTATGTG AAGGGTTGGA TGGTTGGCTT TGAAGAGCAC
    AAGCAAAAGA CAGATGTGCA TTACCGGTCC CTGGGAGGTG AAGGCAACTT TAACTGGAGG
    TTCATTTTCC CCTTTGACTA CCTGCCAGCC GAGCAAGTCT GCACCGTCTC TAAGAAGGAT
    GCTTTCTGGA GGCTGGACAA GACTGAGAGC AAAATCCCAG CACGAGTGGT ATTTCAGATC
    TGGGACAATG ACAAGTTCTC CTTTGACGAC TTTCTGGGCT CCTTGCAGTT GGATCTCAAC
    CACATGCCCA AGCCTGCCAA GACAGCTGAG AAGTGCTCCT TGGACCAGCT GGATGACACT
    TTCCACCCGG AGTGGTTTGT GTCCCTTTTT GAACAGAAAA CAGTTAAGGG CTGGTGGCCC
    TGCGTAGCAG AAGAGGGCGA GAAGAAGATC CTGGCAGGCA AGCTGGAAAT GACCTTGGAG
    ATCGTAGCAG AGAGTGAGCA TGAGGAGCGG CCTGCTGGCC AGGGCCGGGA TGAACCCAAC
    ATGAACCCCA AGCTCGAGGA CCCAAGGCGC CCTGACACCT CCTTCCTCTG GTTCACCTCT
    CCATACAAGA CTATGAAGTT CATCCTGTGG CGGCGGTTCC GATGTGCCAT TATCCTCTTC
    ATCATTCTCT TCATCCTGCT ACTATTCTTA GGCATCTTCA TCTATGCTTT CCCGAACTAC
    GCTGCCATGA AGCTGGTGAA GCCCTTCAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003432272.1
    CDS405..6677
    Translation

    Target ORF information:

    RefSeq Version XM_003432224.4
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris dysferlin (DYSF), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003432224.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC
    CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC
    TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA
    CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGACA CAGGAGGAGA GGAGGACACT
    GAGGACCAGG GGCTCACTGG AGACGAGGGA GAGCCATTAC TGGATCAGAG TGGCGTCCCA
    GGCCCTGGGG CTCCCACCAC CCCGAGGAAG CCACCTTCCC ATCTGCCCCC CTACCACCCT
    GGGAGCAAGA GGAAAAGAAG TGCTCCCGCA GCCAGAAAGC TGCTGTCCGA CAAACCGCAG
    GACTTCCAGA TCAGAGTGCA AGTGATCGAG GGCCGCCAGC TGCCAGGGGT GAATATCAAG
    CCTGTGATCA AGGTCACTGC TGCTGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC
    AGCCCACTAT TTAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTTC GGAGCTGTTT
    GATGAGCCCA TCTTTATCAC GGTCGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT
    GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACATGC TTATCTGAGG
    AAGTGGCTGC TACTCTCCAA CCCTGATGAC TTCTCTGTTG GGGCCAGAGG TTACCTGAAA
    GCAAGCCTTT ATGTGCTGGG GCCCGGAGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT
    GAAGACAAGG AGGACATTGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTGCGAGGA
    GCCCACTTCT GCCTGAAGAT CTTCAGAGCG GAGGACTTAC CACAAATGGA TGATGCCGTG
    ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAATTT GGTAGATCCC
    TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC
    AACCCTCAGT GGAACCAGAG CATCACTCTG CCTGCCATGT TTCCCTCCAT GTGTGAAAAA
    ATGAAGATTC GCGTCATGGA CTGGGACCGC CTTACCCACA ATGACATCGT GGCCACCACC
    TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGTGGA TGACCATCTG
    GGCTTCCTTC CCACCTTCGG GCCCTGCTTC GTCAATCTCT ATGGCAGCCC CAGGGAATTC
    ACTGGCTTCC CGGACCCCTA CGCGGAACTC AACATGGGCA AGGGGGAAGG CGTGGCCTAC
    CGAGGTCGGC TTCTGCTCTC CCTGGAGACC AAGCTGGTGG AGCACAGCGG CGAGCAAAGG
    GTGGAGGACC TCCCTGCTGA TGACATCCTT CGGGTGGAGA AATACCTCCG GAGGCGCAAG
    TACTCTCTCT TTGCCGCCTT CTACTCGGCC ACCATGCTCC AGGATGTCGA CGATGCCATC
    CAGTTTGAGG TCAGCATTGG GAACTATGGC AACAAGTTCG ACACGACCTG TCTGCCACTG
    GCCTCCACCA CCCAGTACAG CCGGGCAGTC TTTGATGGGT GCCACTACTA CTACTTGCCC
    TGGGGCAATG TGAAGCCTGT GGTGGTGCTG TCATCCTACT GGGAAGACAT CAGCCACAGA
    GTCGAGGCTC AGAACCAGCT GCTCAGGATC GCTGACCATC TGGAAGCTGG CCTGGAGCAA
    GTCCACTTGG CTTTAAAGGC GCAGTTCAAC ACAGAGGATG TGGACTCCCT GTTGGCCCAG
    CTGATGGATG AGCTCATCTC AGACTGCAGC CAGCCCCTGG GCAATGTCCA AGAGATACCC
    TCTGCCACCC ACCTGGACCG GCACCTGTAC AGGCTGCGCA CCCGTCACCT AGGCCAGATT
    ACTGAGGCGG CCCTGGCTCT GAAACTCGGG CATGGCAAGC TCCCTGCCGC CCTGGAGCAG
    GCTGAAGACT GGCTCCTGCG TCTCCGTGCC CTGGCTGATG AGCCCCAGAA CAGCCTGCCC
    GACATCATCA TCTGGATGCT GCAGGGAAAC AAGCGAGTGG CGTACCAGCG GGTGCCTGCG
    CACGAGGTCC TCTTCTCCCG GCGGGGCGCC AGCTACTGTG GCAAGAACTG TGGGAAGCTG
    CAGACGATAT TTCTGAAATA TCCCATGGAG GGGGTGCCCG GAGCCCGGAT GCCAGTACAA
    ATACGGGTGA AACTCTGGTT TGGGCTTTCT GTGGATGAGA AGGAGTTCAA CCAGTTTGCT
    GAGGGAAAGC TGTCCGTCTT TGCTGAAACT TATGAGAACC AGACCAAACT GGCCCTGGTT
    GGGAACTGGG GCACAACAGG CCTCACCTAC CCCAAGTTTT CTGATGTCAT GGGCAAGATC
    AAGCTACCCA AGGATAGCTT CCGCCCCTCA GCTGGCTGGA CCTGGGCCGG AGACTGGTTC
    GTGTGTCCAG AGAAGACCCT ACTCCATGAC ATGGACGCGG GCCACCTGAG TTTTGTGGAG
    GAGGTGTTTG AGAACCAGAC CCGGCTCCCT GGAGGCCAGT GGATCTACAT GAGTGACAAC
    TACACAGATG TGAATGGGGA TAAGGTGCTT CCTAAGGATG ACATTGAGTG CCCACTGGGC
    TGGAAGTGGG AAGATGAAGA GTGGTCCACG GACCTCAATC GGGCCGTCGA TGAGCAAGGC
    TGGGAGTACA GTATCACCAT CCCCCCGGAT CGGAAGCCAA AGCACTGGGT TCCTGCTGAG
    AAGATGTATT ACACGCACCG TCGGCGGCGC TGGGTCCGCC TGCGCAGAAG GGACCTCAGC
    CAGATGGAGG CATTGAAGAG GCACAGACAG GCAGAGGCAG AGGGTGAGGG CTGGGAGTAC
    GCCTCGCTCT TGGGCTGGAA GTTCCACCTG GAGTACCGCA AGACTGATGC TTTCCGCCGC
    CGCCGCTGGC GCCGCCGCAT GGAACCGCTG GAGAAGACGG GGCCTGCAGC CGTGTTTGCC
    CTTGAGGGGG CTCTGGGTGG TGTGGTAGAT GACAGGAGTG AAGATTCCGT GTCCGTGTCC
    ACCTTGAGCT TTGGAGTGAA CAGACCCACG ATTTCCTGCA TCTTTGATTA TGGGAACCGC
    TACCACCTAC GCTGCTACAT GTACCAGGCT CGGGACCTGC CCTCGATGGA CAAGGACTCT
    TTTTCTGATC CCTACGCCAT CGTCTCCTTC CTGCACCAGA GCCAGAAGAC AGTGGTGGCG
    AAGAACACTC TGAACCCCAC CTGGGACCAG ACGCTTATCT TCTATGAGAT TGAGATCTTT
    GGTGAGCCAG CCAGTATCGC TGAGCAGCCG CCCAGCATTG TGGTGGAGCT GTATGACCAC
    GACACCTACG GTGCAGATGA GTTTATGGGA CGCTGCATTT GTCAGCCCAG CCTGGAGCGG
    ACACCCCGGC TGGCCTGGTT CCCACTGATT AGGGGCAGCC AGTCGGCGGG GGAACTGCTG
    GCCTCTTTTG AGCTCATCCA GAGAGAAAAG CCGGCCATTC ACCATATTCC TGGTTTTGAG
    GTACAGGATA TGTCAGGAAT CCTGGAAGAG TCAGAGGACA TAGACCTGCC CTACCCGCCA
    CCTCAGAGGG AGGCCAACAT CTTCATGGTC CCCCAGAACA TCAAGCCTGT TCTCCAGCGT
    ACTGCCATAG AGATCCTGGC GTGGGGCCTG CGGAACATGA AGAGCTACCA GCTAGCCAGC
    ATCTCCTCCC CTAGCCTTGT GGTAGAGTGC GGGGGCCAGA CAGTGCAGTC CTGTGTCATC
    AAGAACCTCC GGAAGAACCC CAACTTCGAC ATCTGTACCC TTTTCATGGA AGTGATGCTG
    CCCAAGGAGG AGGTCTACTG TCCCTCCATC GTGATCAAGG TCATTGATAA CCGCCAGTTT
    GGCCGCCGGC CTGTGGTGGG CCAGTGTACC ATCCGTTCCC TGGAGAGCTT CATGTGTGAC
    CTGTACTCCG AGGAGAATCC GTCCCCACAG GGCGGCCCAG ATGATGTGAG TTTACTCAGT
    CCTGGGGAAG ACGTGCTCAT TGACATCGAT GACAAGGAGC CCCTCATCCC CATGCAGCTT
    GCAGATGGTC TGTCGGGCTC TGCCCCCACT AACATGGCAT CTTCTCCATC CAGTCCTCAT
    GAGGAAGAGT TCATCGACTG GTGGAGCAAA TTCTTTGCCT CCACAGGGGA GAGGGAAAAG
    TGCGGCTCAT ACTTGGAGAA GGGCTTTGAC ACTCTAAAGG TCTATGACAC ACAACTGGAG
    AACGTGGAGG CCTTTGAGGG CCTGTCTGAC TTTTGTAACA CCTTCAAGCT CTACCGGGGC
    AAGACTCAGG AGGAGACAGA GGACCCATCT GTTATTGGGG AGTTTAAGGG CCTCTTCAAA
    ATCTATCCCC TCCCAGAAGA CCCGGCTGTT CCCATGCCCC CGAGACAATT CCACCAGCTG
    GCTGCCCAAG GGCCTCAGGA GTGCCTGGTC CGCATCTATA TCATCCGAGC ATTTGGCCTT
    CAGCCCAAGG ACCCCAATGG GAAGTGTGAT CCTTACATCA AGATCTCCAT AGGGAAGAAA
    TCAGTGAGTG ACCAGGATAA CTACATTCCC TGTACTCTGG AGCCTGTGTT TGGAAAGATG
    TTCGAGCTGA CCTGCACTCT GCCTCTGGAG AAGGACCTGA AGATCACACT CTATGACTAT
    GACCTCCTCT CCAAAGATGA AAAGATTGGG GAGACAGTCA TTGACCTGGA GGATAGGCTG
    CTGTCCAAGT TTGGGGCTCG CTGCGGACTC CCACAGACGT ACTGTGTCTC TGGACCCAAC
    CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG CTTCTCCACC TCTTCTGCCA GCAGCGCAGA
    CTCAAGGCTC CTGTGTACCT CACGGACCAC GTGGTGTTCC AGGATAAAGA ATACAGCATT
    GAAGAAATAG AGGCTGGCCG AGTCCCGAAC CCACACCTGG GCCCAGTGGA AGAGCGTCTG
    GCCTTGCATG TCCTCCAGCA GCAGGGCCTG GTCCCTGAGC ATGTGGAGTC CCGGCCTCTC
    TACAGCCCCC TGCAGCCAGA CATTGAACAG GGGAAGCTGC AGATGTGGAT CGACCTATTT
    CCCAAGGCCC TGGGGCGTCC CGGACCTCCC TTCAACATCA CCCCGCGGAG AGCCAGAAGG
    TTTTTCCTGC GTTGTATCAT CTGGAACACA AAGGACGTGA TCCTGGACGA CCTCAGCATC
    ACAGGGGAGA AGATGAGTGA TATTTATGTG AAGGGTTGGA TGGTTGGCTT TGAAGAGCAC
    AAGCAAAAGA CAGATGTGCA TTACCGGTCC CTGGGAGGTG AAGGCAACTT TAACTGGAGG
    TTCATTTTCC CCTTTGACTA CCTGCCAGCC GAGCAAGTCT GCACCGTCTC TAAGAAGGAT
    GCTTTCTGGA GGCTGGACAA GACTGAGAGC AAAATCCCAG CACGAGTGGT ATTTCAGATC
    TGGGACAATG ACAAGTTCTC CTTTGACGAC TTTCTGGGCT CCTTGCAGTT GGATCTCAAC
    CACATGCCCA AGCCTGCCAA GACAGCTGAG AAGTGCTCCT TGGACCAGCT GGATGACACT
    TTCCACCCGG AGTGGTTTGT GTCCCTTTTT GAACAGAAAA CAGTTAAGGG CTGGTGGCCC
    TGCGTAGCAG AAGAGGGCGA GAAGAAGATC CTGGCAGGCA AGCTGGAAAT GACCTTGGAG
    ATCGTAGCAG AGAGTGAGCA TGAGGAGCGG CCTGCTGGCC AGGGCCGGGA TGAACCCAAC
    ATGAACCCCA AGCTCGAGGA CCCAAGGCGC CCTGACACCT CCTTCCTCTG GTTCACCTCT
    CCATACAAGA CTATGAAGTT CATCCTGTGG CGGCGGTTCC GATGTGCCAT TATCCTCTTC
    ATCATTCTCT TCATCCTGCT ACTATTCTTA GGCATCTTCA TCTATGCTTT CCCGAACTAC
    GCTGCCATGA AGCTGGTGAA GCCCTTCAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe19215
    Clone ID Related Accession (Same CDS sequence) XM_003432224.4 , XM_003432224.3
    Accession Version XM_003432224.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6273bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product dysferlin isoform X10
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003432224.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC
    CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC
    TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA
    CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGACA CAGGAGGAGA GGAGGACACT
    GAGGACCAGG GGCTCACTGG AGACGAGGGA GAGCCATTAC TGGATCAGAG TGGCGTCCCA
    GGCCCTGGGG CTCCCACCAC CCCGAGGAAG CCACCTTCCC ATCTGCCCCC CTACCACCCT
    GGGAGCAAGA GGAAAAGAAG TGCTCCCGCA GCCAGAAAGC TGCTGTCCGA CAAACCGCAG
    GACTTCCAGA TCAGAGTGCA AGTGATCGAG GGCCGCCAGC TGCCAGGGGT GAATATCAAG
    CCTGTGATCA AGGTCACTGC TGCTGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC
    AGCCCACTAT TTAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTTC GGAGCTGTTT
    GATGAGCCCA TCTTTATCAC GGTCGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT
    GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACATGC TTATCTGAGG
    AAGTGGCTGC TACTCTCCAA CCCTGATGAC TTCTCTGTTG GGGCCAGAGG TTACCTGAAA
    GCAAGCCTTT ATGTGCTGGG GCCCGGAGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT
    GAAGACAAGG AGGACATTGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTGCGAGGA
    GCCCACTTCT GCCTGAAGAT CTTCAGAGCG GAGGACTTAC CACAAATGGA TGATGCCGTG
    ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAATTT GGTAGATCCC
    TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC
    AACCCTCAGT GGAACCAGAG CATCACTCTG CCTGCCATGT TTCCCTCCAT GTGTGAAAAA
    ATGAAGATTC GCGTCATGGA CTGGGACCGC CTTACCCACA ATGACATCGT GGCCACCACC
    TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGTGGA TGACCATCTG
    GGCTTCCTTC CCACCTTCGG GCCCTGCTTC GTCAATCTCT ATGGCAGCCC CAGGGAATTC
    ACTGGCTTCC CGGACCCCTA CGCGGAACTC AACATGGGCA AGGGGGAAGG CGTGGCCTAC
    CGAGGTCGGC TTCTGCTCTC CCTGGAGACC AAGCTGGTGG AGCACAGCGG CGAGCAAAGG
    GTGGAGGACC TCCCTGCTGA TGACATCCTT CGGGTGGAGA AATACCTCCG GAGGCGCAAG
    TACTCTCTCT TTGCCGCCTT CTACTCGGCC ACCATGCTCC AGGATGTCGA CGATGCCATC
    CAGTTTGAGG TCAGCATTGG GAACTATGGC AACAAGTTCG ACACGACCTG TCTGCCACTG
    GCCTCCACCA CCCAGTACAG CCGGGCAGTC TTTGATGGGT GCCACTACTA CTACTTGCCC
    TGGGGCAATG TGAAGCCTGT GGTGGTGCTG TCATCCTACT GGGAAGACAT CAGCCACAGA
    GTCGAGGCTC AGAACCAGCT GCTCAGGATC GCTGACCATC TGGAAGCTGG CCTGGAGCAA
    GTCCACTTGG CTTTAAAGGC GCAGTTCAAC ACAGAGGATG TGGACTCCCT GTTGGCCCAG
    CTGATGGATG AGCTCATCTC AGACTGCAGC CAGCCCCTGG GCAATGTCCA AGAGATACCC
    TCTGCCACCC ACCTGGACCG GCACCTGTAC AGGCTGCGCA CCCGTCACCT AGGCCAGATT
    ACTGAGGCGG CCCTGGCTCT GAAACTCGGG CATGGCAAGC TCCCTGCCGC CCTGGAGCAG
    GCTGAAGACT GGCTCCTGCG TCTCCGTGCC CTGGCTGATG AGCCCCAGAA CAGCCTGCCC
    GACATCATCA TCTGGATGCT GCAGGGAAAC AAGCGAGTGG CGTACCAGCG GGTGCCTGCG
    CACGAGGTCC TCTTCTCCCG GCGGGGCGCC AGCTACTGTG GCAAGAACTG TGGGAAGCTG
    CAGACGATAT TTCTGAAATA TCCCATGGAG GGGGTGCCCG GAGCCCGGAT GCCAGTACAA
    ATACGGGTGA AACTCTGGTT TGGGCTTTCT GTGGATGAGA AGGAGTTCAA CCAGTTTGCT
    GAGGGAAAGC TGTCCGTCTT TGCTGAAACT TATGAGAACC AGACCAAACT GGCCCTGGTT
    GGGAACTGGG GCACAACAGG CCTCACCTAC CCCAAGTTTT CTGATGTCAT GGGCAAGATC
    AAGCTACCCA AGGATAGCTT CCGCCCCTCA GCTGGCTGGA CCTGGGCCGG AGACTGGTTC
    GTGTGTCCAG AGAAGACCCT ACTCCATGAC ATGGACGCGG GCCACCTGAG TTTTGTGGAG
    GAGGTGTTTG AGAACCAGAC CCGGCTCCCT GGAGGCCAGT GGATCTACAT GAGTGACAAC
    TACACAGATG TGAATGGGGA TAAGGTGCTT CCTAAGGATG ACATTGAGTG CCCACTGGGC
    TGGAAGTGGG AAGATGAAGA GTGGTCCACG GACCTCAATC GGGCCGTCGA TGAGCAAGGC
    TGGGAGTACA GTATCACCAT CCCCCCGGAT CGGAAGCCAA AGCACTGGGT TCCTGCTGAG
    AAGATGTATT ACACGCACCG TCGGCGGCGC TGGGTCCGCC TGCGCAGAAG GGACCTCAGC
    CAGATGGAGG CATTGAAGAG GCACAGACAG GCAGAGGCAG AGGGTGAGGG CTGGGAGTAC
    GCCTCGCTCT TGGGCTGGAA GTTCCACCTG GAGTACCGCA AGACTGATGC TTTCCGCCGC
    CGCCGCTGGC GCCGCCGCAT GGAACCGCTG GAGAAGACGG GGCCTGCAGC CGTGTTTGCC
    CTTGAGGGGG CTCTGGGTGG TGTGGTAGAT GACAGGAGTG AAGATTCCGT GTCCGTGTCC
    ACCTTGAGCT TTGGAGTGAA CAGACCCACG ATTTCCTGCA TCTTTGATTA TGGGAACCGC
    TACCACCTAC GCTGCTACAT GTACCAGGCT CGGGACCTGC CCTCGATGGA CAAGGACTCT
    TTTTCTGATC CCTACGCCAT CGTCTCCTTC CTGCACCAGA GCCAGAAGAC AGTGGTGGCG
    AAGAACACTC TGAACCCCAC CTGGGACCAG ACGCTTATCT TCTATGAGAT TGAGATCTTT
    GGTGAGCCAG CCAGTATCGC TGAGCAGCCG CCCAGCATTG TGGTGGAGCT GTATGACCAC
    GACACCTACG GTGCAGATGA GTTTATGGGA CGCTGCATTT GTCAGCCCAG CCTGGAGCGG
    ACACCCCGGC TGGCCTGGTT CCCACTGATT AGGGGCAGCC AGTCGGCGGG GGAACTGCTG
    GCCTCTTTTG AGCTCATCCA GAGAGAAAAG CCGGCCATTC ACCATATTCC TGGTTTTGAG
    GTACAGGATA TGTCAGGAAT CCTGGAAGAG TCAGAGGACA TAGACCTGCC CTACCCGCCA
    CCTCAGAGGG AGGCCAACAT CTTCATGGTC CCCCAGAACA TCAAGCCTGT TCTCCAGCGT
    ACTGCCATAG AGATCCTGGC GTGGGGCCTG CGGAACATGA AGAGCTACCA GCTAGCCAGC
    ATCTCCTCCC CTAGCCTTGT GGTAGAGTGC GGGGGCCAGA CAGTGCAGTC CTGTGTCATC
    AAGAACCTCC GGAAGAACCC CAACTTCGAC ATCTGTACCC TTTTCATGGA AGTGATGCTG
    CCCAAGGAGG AGGTCTACTG TCCCTCCATC GTGATCAAGG TCATTGATAA CCGCCAGTTT
    GGCCGCCGGC CTGTGGTGGG CCAGTGTACC ATCCGTTCCC TGGAGAGCTT CATGTGTGAC
    CTGTACTCCG AGGAGAATCC GTCCCCACAG GGCGGCCCAG ATGATGTGAG TTTACTCAGT
    CCTGGGGAAG ACGTGCTCAT TGACATCGAT GACAAGGAGC CCCTCATCCC CATGCAGCTT
    GCAGATGGTC TGTCGGGCTC TGCCCCCACT AACATGGCAT CTTCTCCATC CAGTCCTCAT
    GAGGAAGAGT TCATCGACTG GTGGAGCAAA TTCTTTGCCT CCACAGGGGA GAGGGAAAAG
    TGCGGCTCAT ACTTGGAGAA GGGCTTTGAC ACTCTAAAGG TCTATGACAC ACAACTGGAG
    AACGTGGAGG CCTTTGAGGG CCTGTCTGAC TTTTGTAACA CCTTCAAGCT CTACCGGGGC
    AAGACTCAGG AGGAGACAGA GGACCCATCT GTTATTGGGG AGTTTAAGGG CCTCTTCAAA
    ATCTATCCCC TCCCAGAAGA CCCGGCTGTT CCCATGCCCC CGAGACAATT CCACCAGCTG
    GCTGCCCAAG GGCCTCAGGA GTGCCTGGTC CGCATCTATA TCATCCGAGC ATTTGGCCTT
    CAGCCCAAGG ACCCCAATGG GAAGTGTGAT CCTTACATCA AGATCTCCAT AGGGAAGAAA
    TCAGTGAGTG ACCAGGATAA CTACATTCCC TGTACTCTGG AGCCTGTGTT TGGAAAGATG
    TTCGAGCTGA CCTGCACTCT GCCTCTGGAG AAGGACCTGA AGATCACACT CTATGACTAT
    GACCTCCTCT CCAAAGATGA AAAGATTGGG GAGACAGTCA TTGACCTGGA GGATAGGCTG
    CTGTCCAAGT TTGGGGCTCG CTGCGGACTC CCACAGACGT ACTGTGTCTC TGGACCCAAC
    CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG CTTCTCCACC TCTTCTGCCA GCAGCGCAGA
    CTCAAGGCTC CTGTGTACCT CACGGACCAC GTGGTGTTCC AGGATAAAGA ATACAGCATT
    GAAGAAATAG AGGCTGGCCG AGTCCCGAAC CCACACCTGG GCCCAGTGGA AGAGCGTCTG
    GCCTTGCATG TCCTCCAGCA GCAGGGCCTG GTCCCTGAGC ATGTGGAGTC CCGGCCTCTC
    TACAGCCCCC TGCAGCCAGA CATTGAACAG GGGAAGCTGC AGATGTGGAT CGACCTATTT
    CCCAAGGCCC TGGGGCGTCC CGGACCTCCC TTCAACATCA CCCCGCGGAG AGCCAGAAGG
    TTTTTCCTGC GTTGTATCAT CTGGAACACA AAGGACGTGA TCCTGGACGA CCTCAGCATC
    ACAGGGGAGA AGATGAGTGA TATTTATGTG AAGGGTTGGA TGGTTGGCTT TGAAGAGCAC
    AAGCAAAAGA CAGATGTGCA TTACCGGTCC CTGGGAGGTG AAGGCAACTT TAACTGGAGG
    TTCATTTTCC CCTTTGACTA CCTGCCAGCC GAGCAAGTCT GCACCGTCTC TAAGAAGGAT
    GCTTTCTGGA GGCTGGACAA GACTGAGAGC AAAATCCCAG CACGAGTGGT ATTTCAGATC
    TGGGACAATG ACAAGTTCTC CTTTGACGAC TTTCTGGGCT CCTTGCAGTT GGATCTCAAC
    CACATGCCCA AGCCTGCCAA GACAGCTGAG AAGTGCTCCT TGGACCAGCT GGATGACACT
    TTCCACCCGG AGTGGTTTGT GTCCCTTTTT GAACAGAAAA CAGTTAAGGG CTGGTGGCCC
    TGCGTAGCAG AAGAGGGCGA GAAGAAGATC CTGGCAGGCA AGCTGGAAAT GACCTTGGAG
    ATCGTAGCAG AGAGTGAGCA TGAGGAGCGG CCTGCTGGCC AGGGCCGGGA TGAACCCAAC
    ATGAACCCCA AGCTCGAGGA CCCAAGGCGC CCTGACACCT CCTTCCTCTG GTTCACCTCT
    CCATACAAGA CTATGAAGTT CATCCTGTGG CGGCGGTTCC GATGTGCCAT TATCCTCTTC
    ATCATTCTCT TCATCCTGCT ACTATTCTTA GGCATCTTCA TCTATGCTTT CCCGAACTAC
    GCTGCCATGA AGCTGGTGAA GCCCTTCAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003432272.1
    CDS439..6711
    Translation

    Target ORF information:

    RefSeq Version XM_003432224.3
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris dysferlin (DYSF), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003432224.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC
    CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC
    TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA
    CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGACA CAGGAGGAGA GGAGGACACT
    GAGGACCAGG GGCTCACTGG AGACGAGGGA GAGCCATTAC TGGATCAGAG TGGCGTCCCA
    GGCCCTGGGG CTCCCACCAC CCCGAGGAAG CCACCTTCCC ATCTGCCCCC CTACCACCCT
    GGGAGCAAGA GGAAAAGAAG TGCTCCCGCA GCCAGAAAGC TGCTGTCCGA CAAACCGCAG
    GACTTCCAGA TCAGAGTGCA AGTGATCGAG GGCCGCCAGC TGCCAGGGGT GAATATCAAG
    CCTGTGATCA AGGTCACTGC TGCTGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC
    AGCCCACTAT TTAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTTC GGAGCTGTTT
    GATGAGCCCA TCTTTATCAC GGTCGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT
    GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACATGC TTATCTGAGG
    AAGTGGCTGC TACTCTCCAA CCCTGATGAC TTCTCTGTTG GGGCCAGAGG TTACCTGAAA
    GCAAGCCTTT ATGTGCTGGG GCCCGGAGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT
    GAAGACAAGG AGGACATTGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTGCGAGGA
    GCCCACTTCT GCCTGAAGAT CTTCAGAGCG GAGGACTTAC CACAAATGGA TGATGCCGTG
    ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAATTT GGTAGATCCC
    TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC
    AACCCTCAGT GGAACCAGAG CATCACTCTG CCTGCCATGT TTCCCTCCAT GTGTGAAAAA
    ATGAAGATTC GCGTCATGGA CTGGGACCGC CTTACCCACA ATGACATCGT GGCCACCACC
    TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGTGGA TGACCATCTG
    GGCTTCCTTC CCACCTTCGG GCCCTGCTTC GTCAATCTCT ATGGCAGCCC CAGGGAATTC
    ACTGGCTTCC CGGACCCCTA CGCGGAACTC AACATGGGCA AGGGGGAAGG CGTGGCCTAC
    CGAGGTCGGC TTCTGCTCTC CCTGGAGACC AAGCTGGTGG AGCACAGCGG CGAGCAAAGG
    GTGGAGGACC TCCCTGCTGA TGACATCCTT CGGGTGGAGA AATACCTCCG GAGGCGCAAG
    TACTCTCTCT TTGCCGCCTT CTACTCGGCC ACCATGCTCC AGGATGTCGA CGATGCCATC
    CAGTTTGAGG TCAGCATTGG GAACTATGGC AACAAGTTCG ACACGACCTG TCTGCCACTG
    GCCTCCACCA CCCAGTACAG CCGGGCAGTC TTTGATGGGT GCCACTACTA CTACTTGCCC
    TGGGGCAATG TGAAGCCTGT GGTGGTGCTG TCATCCTACT GGGAAGACAT CAGCCACAGA
    GTCGAGGCTC AGAACCAGCT GCTCAGGATC GCTGACCATC TGGAAGCTGG CCTGGAGCAA
    GTCCACTTGG CTTTAAAGGC GCAGTTCAAC ACAGAGGATG TGGACTCCCT GTTGGCCCAG
    CTGATGGATG AGCTCATCTC AGACTGCAGC CAGCCCCTGG GCAATGTCCA AGAGATACCC
    TCTGCCACCC ACCTGGACCG GCACCTGTAC AGGCTGCGCA CCCGTCACCT AGGCCAGATT
    ACTGAGGCGG CCCTGGCTCT GAAACTCGGG CATGGCAAGC TCCCTGCCGC CCTGGAGCAG
    GCTGAAGACT GGCTCCTGCG TCTCCGTGCC CTGGCTGATG AGCCCCAGAA CAGCCTGCCC
    GACATCATCA TCTGGATGCT GCAGGGAAAC AAGCGAGTGG CGTACCAGCG GGTGCCTGCG
    CACGAGGTCC TCTTCTCCCG GCGGGGCGCC AGCTACTGTG GCAAGAACTG TGGGAAGCTG
    CAGACGATAT TTCTGAAATA TCCCATGGAG GGGGTGCCCG GAGCCCGGAT GCCAGTACAA
    ATACGGGTGA AACTCTGGTT TGGGCTTTCT GTGGATGAGA AGGAGTTCAA CCAGTTTGCT
    GAGGGAAAGC TGTCCGTCTT TGCTGAAACT TATGAGAACC AGACCAAACT GGCCCTGGTT
    GGGAACTGGG GCACAACAGG CCTCACCTAC CCCAAGTTTT CTGATGTCAT GGGCAAGATC
    AAGCTACCCA AGGATAGCTT CCGCCCCTCA GCTGGCTGGA CCTGGGCCGG AGACTGGTTC
    GTGTGTCCAG AGAAGACCCT ACTCCATGAC ATGGACGCGG GCCACCTGAG TTTTGTGGAG
    GAGGTGTTTG AGAACCAGAC CCGGCTCCCT GGAGGCCAGT GGATCTACAT GAGTGACAAC
    TACACAGATG TGAATGGGGA TAAGGTGCTT CCTAAGGATG ACATTGAGTG CCCACTGGGC
    TGGAAGTGGG AAGATGAAGA GTGGTCCACG GACCTCAATC GGGCCGTCGA TGAGCAAGGC
    TGGGAGTACA GTATCACCAT CCCCCCGGAT CGGAAGCCAA AGCACTGGGT TCCTGCTGAG
    AAGATGTATT ACACGCACCG TCGGCGGCGC TGGGTCCGCC TGCGCAGAAG GGACCTCAGC
    CAGATGGAGG CATTGAAGAG GCACAGACAG GCAGAGGCAG AGGGTGAGGG CTGGGAGTAC
    GCCTCGCTCT TGGGCTGGAA GTTCCACCTG GAGTACCGCA AGACTGATGC TTTCCGCCGC
    CGCCGCTGGC GCCGCCGCAT GGAACCGCTG GAGAAGACGG GGCCTGCAGC CGTGTTTGCC
    CTTGAGGGGG CTCTGGGTGG TGTGGTAGAT GACAGGAGTG AAGATTCCGT GTCCGTGTCC
    ACCTTGAGCT TTGGAGTGAA CAGACCCACG ATTTCCTGCA TCTTTGATTA TGGGAACCGC
    TACCACCTAC GCTGCTACAT GTACCAGGCT CGGGACCTGC CCTCGATGGA CAAGGACTCT
    TTTTCTGATC CCTACGCCAT CGTCTCCTTC CTGCACCAGA GCCAGAAGAC AGTGGTGGCG
    AAGAACACTC TGAACCCCAC CTGGGACCAG ACGCTTATCT TCTATGAGAT TGAGATCTTT
    GGTGAGCCAG CCAGTATCGC TGAGCAGCCG CCCAGCATTG TGGTGGAGCT GTATGACCAC
    GACACCTACG GTGCAGATGA GTTTATGGGA CGCTGCATTT GTCAGCCCAG CCTGGAGCGG
    ACACCCCGGC TGGCCTGGTT CCCACTGATT AGGGGCAGCC AGTCGGCGGG GGAACTGCTG
    GCCTCTTTTG AGCTCATCCA GAGAGAAAAG CCGGCCATTC ACCATATTCC TGGTTTTGAG
    GTACAGGATA TGTCAGGAAT CCTGGAAGAG TCAGAGGACA TAGACCTGCC CTACCCGCCA
    CCTCAGAGGG AGGCCAACAT CTTCATGGTC CCCCAGAACA TCAAGCCTGT TCTCCAGCGT
    ACTGCCATAG AGATCCTGGC GTGGGGCCTG CGGAACATGA AGAGCTACCA GCTAGCCAGC
    ATCTCCTCCC CTAGCCTTGT GGTAGAGTGC GGGGGCCAGA CAGTGCAGTC CTGTGTCATC
    AAGAACCTCC GGAAGAACCC CAACTTCGAC ATCTGTACCC TTTTCATGGA AGTGATGCTG
    CCCAAGGAGG AGGTCTACTG TCCCTCCATC GTGATCAAGG TCATTGATAA CCGCCAGTTT
    GGCCGCCGGC CTGTGGTGGG CCAGTGTACC ATCCGTTCCC TGGAGAGCTT CATGTGTGAC
    CTGTACTCCG AGGAGAATCC GTCCCCACAG GGCGGCCCAG ATGATGTGAG TTTACTCAGT
    CCTGGGGAAG ACGTGCTCAT TGACATCGAT GACAAGGAGC CCCTCATCCC CATGCAGCTT
    GCAGATGGTC TGTCGGGCTC TGCCCCCACT AACATGGCAT CTTCTCCATC CAGTCCTCAT
    GAGGAAGAGT TCATCGACTG GTGGAGCAAA TTCTTTGCCT CCACAGGGGA GAGGGAAAAG
    TGCGGCTCAT ACTTGGAGAA GGGCTTTGAC ACTCTAAAGG TCTATGACAC ACAACTGGAG
    AACGTGGAGG CCTTTGAGGG CCTGTCTGAC TTTTGTAACA CCTTCAAGCT CTACCGGGGC
    AAGACTCAGG AGGAGACAGA GGACCCATCT GTTATTGGGG AGTTTAAGGG CCTCTTCAAA
    ATCTATCCCC TCCCAGAAGA CCCGGCTGTT CCCATGCCCC CGAGACAATT CCACCAGCTG
    GCTGCCCAAG GGCCTCAGGA GTGCCTGGTC CGCATCTATA TCATCCGAGC ATTTGGCCTT
    CAGCCCAAGG ACCCCAATGG GAAGTGTGAT CCTTACATCA AGATCTCCAT AGGGAAGAAA
    TCAGTGAGTG ACCAGGATAA CTACATTCCC TGTACTCTGG AGCCTGTGTT TGGAAAGATG
    TTCGAGCTGA CCTGCACTCT GCCTCTGGAG AAGGACCTGA AGATCACACT CTATGACTAT
    GACCTCCTCT CCAAAGATGA AAAGATTGGG GAGACAGTCA TTGACCTGGA GGATAGGCTG
    CTGTCCAAGT TTGGGGCTCG CTGCGGACTC CCACAGACGT ACTGTGTCTC TGGACCCAAC
    CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG CTTCTCCACC TCTTCTGCCA GCAGCGCAGA
    CTCAAGGCTC CTGTGTACCT CACGGACCAC GTGGTGTTCC AGGATAAAGA ATACAGCATT
    GAAGAAATAG AGGCTGGCCG AGTCCCGAAC CCACACCTGG GCCCAGTGGA AGAGCGTCTG
    GCCTTGCATG TCCTCCAGCA GCAGGGCCTG GTCCCTGAGC ATGTGGAGTC CCGGCCTCTC
    TACAGCCCCC TGCAGCCAGA CATTGAACAG GGGAAGCTGC AGATGTGGAT CGACCTATTT
    CCCAAGGCCC TGGGGCGTCC CGGACCTCCC TTCAACATCA CCCCGCGGAG AGCCAGAAGG
    TTTTTCCTGC GTTGTATCAT CTGGAACACA AAGGACGTGA TCCTGGACGA CCTCAGCATC
    ACAGGGGAGA AGATGAGTGA TATTTATGTG AAGGGTTGGA TGGTTGGCTT TGAAGAGCAC
    AAGCAAAAGA CAGATGTGCA TTACCGGTCC CTGGGAGGTG AAGGCAACTT TAACTGGAGG
    TTCATTTTCC CCTTTGACTA CCTGCCAGCC GAGCAAGTCT GCACCGTCTC TAAGAAGGAT
    GCTTTCTGGA GGCTGGACAA GACTGAGAGC AAAATCCCAG CACGAGTGGT ATTTCAGATC
    TGGGACAATG ACAAGTTCTC CTTTGACGAC TTTCTGGGCT CCTTGCAGTT GGATCTCAAC
    CACATGCCCA AGCCTGCCAA GACAGCTGAG AAGTGCTCCT TGGACCAGCT GGATGACACT
    TTCCACCCGG AGTGGTTTGT GTCCCTTTTT GAACAGAAAA CAGTTAAGGG CTGGTGGCCC
    TGCGTAGCAG AAGAGGGCGA GAAGAAGATC CTGGCAGGCA AGCTGGAAAT GACCTTGGAG
    ATCGTAGCAG AGAGTGAGCA TGAGGAGCGG CCTGCTGGCC AGGGCCGGGA TGAACCCAAC
    ATGAACCCCA AGCTCGAGGA CCCAAGGCGC CCTGACACCT CCTTCCTCTG GTTCACCTCT
    CCATACAAGA CTATGAAGTT CATCCTGTGG CGGCGGTTCC GATGTGCCAT TATCCTCTTC
    ATCATTCTCT TCATCCTGCT ACTATTCTTA GGCATCTTCA TCTATGCTTT CCCGAACTAC
    GCTGCCATGA AGCTGGTGAA GCCCTTCAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe19216
    Clone ID Related Accession (Same CDS sequence) XM_003432227.3 , XM_003432227.4
    Accession Version XM_003432227.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6255bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product dysferlin isoform X11
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003432227.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG ACACAGGAGG AGAGGAGGAC
    ACTGAGGACC AGGGGCTCAC TGGAGACGAG GGAGAGCCAT TACTGGATCA GAGTGGCGTC
    CCAGGCCCTG GGGCTCCCAC CACCCCGAGG AAGCCACCTT CCCATCTGCC CCCCTACCAC
    CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC GCAGCCAGAA AGCTGCTGTC CGACAAACCG
    CAGGACTTCC AGATCAGAGT GCAAGTGATC GAGGGCCGCC AGCTGCCAGG GGTGAATATC
    AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG CAGACCAAGA GGACACGGAT CCACAAGGGA
    AACAGCCCAC TATTTAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TTCGGAGCTG
    TTTGATGAGC CCATCTTTAT CACGGTCGTA GACTCCCGTT CTCTCAGGAC AGATGCTCTC
    ATTGGGGAAT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA TGCTTATCTG
    AGGAAGTGGC TGCTACTCTC CAACCCTGAT GACTTCTCTG TTGGGGCCAG AGGTTACCTG
    AAAGCAAGCC TTTATGTGCT GGGGCCCGGA GATGAAGCGC CTCTGGAGAG AAAGGACCCC
    TCTGAAGACA AGGAGGACAT TGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTGCGA
    GGAGCCCACT TCTGCCTGAA GATCTTCAGA GCGGAGGACT TACCACAAAT GGATGATGCC
    GTGATGGACA ATGTGAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA TTTGGTAGAT
    CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA
    GCCAACCCTC AGTGGAACCA GAGCATCACT CTGCCTGCCA TGTTTCCCTC CATGTGTGAA
    AAAATGAAGA TTCGCGTCAT GGACTGGGAC CGCCTTACCC ACAATGACAT CGTGGCCACC
    ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGAAGA GGAGCCTGCA
    GGTGTGGTCA AGCCTTCTCC AGCCACGGAC CTGGATGACC ATCTGGGCTT CCTTCCCACC
    TTCGGGCCCT GCTTCGTCAA TCTCTATGGC AGCCCCAGGG AATTCACTGG CTTCCCGGAC
    CCCTACGCGG AACTCAACAT GGGCAAGGGG GAAGGCGTGG CCTACCGAGG TCGGCTTCTG
    CTCTCCCTGG AGACCAAGCT GGTGGAGCAC AGCGGCGAGC AAAGGGTGGA GGACCTCCCT
    GCTGATGACA TCCTTCGGGT GGAGAAATAC CTCCGGAGGC GCAAGTACTC TCTCTTTGCC
    GCCTTCTACT CGGCCACCAT GCTCCAGGAT GTCGACGATG CCATCCAGTT TGAGGTCAGC
    ATTGGGAACT ATGGCAACAA GTTCGACACG ACCTGTCTGC CACTGGCCTC CACCACCCAG
    TACAGCCGGG CAGTCTTTGA TGGGTGCCAC TACTACTACT TGCCCTGGGG CAATGTGAAG
    CCTGTGGTGG TGCTGTCATC CTACTGGGAA GACATCAGCC ACAGAGTCGA GGCTCAGAAC
    CAGCTGCTCA GGATCGCTGA CCATCTGGAA GCTGGCCTGG AGCAAGTCCA CTTGGCTTTA
    AAGGCGCAGT TCAACACAGA GGATGTGGAC TCCCTGTTGG CCCAGCTGAT GGATGAGCTC
    ATCTCAGACT GCAGCCAGCC CCTGGGCAAT GTCCAAGAGA TACCCTCTGC CACCCACCTG
    GACCGGCACC TGTACAGGCT GCGCACCCGT CACCTAGGCC AGATTACTGA GGCGGCCCTG
    GCTCTGAAAC TCGGGCATGG CAAGCTCCCT GCCGCCCTGG AGCAGGCTGA AGACTGGCTC
    CTGCGTCTCC GTGCCCTGGC TGATGAGCCC CAGAACAGCC TGCCCGACAT CATCATCTGG
    ATGCTGCAGG GAAACAAGCG AGTGGCGTAC CAGCGGGTGC CTGCGCACGA GGTCCTCTTC
    TCCCGGCGGG GCGCCAGCTA CTGTGGCAAG AACTGTGGGA AGCTGCAGAC GATATTTCTG
    AAATATCCCA TGGAGGGGGT GCCCGGAGCC CGGATGCCAG TACAAATACG GGTGAAACTC
    TGGTTTGGGC TTTCTGTGGA TGAGAAGGAG TTCAACCAGT TTGCTGAGGG AAAGCTGTCC
    GTCTTTGCTG AAACTTATGA GAACCAGACC AAACTGGCCC TGGTTGGGAA CTGGGGCACA
    ACAGGCCTCA CCTACCCCAA GTTTTCTGAT GTCATGGGCA AGATCAAGCT ACCCAAGGAT
    AGCTTCCGCC CCTCAGCTGG CTGGACCTGG GCCGGAGACT GGTTCGTGTG TCCAGAGAAG
    ACCCTACTCC ATGACATGGA CGCGGGCCAC CTGAGTTTTG TGGAGGAGGT GTTTGAGAAC
    CAGACCCGGC TCCCTGGAGG CCAGTGGATC TACATGAGTG ACAACTACAC AGATGTGAAT
    GGGGATAAGG TGCTTCCTAA GGATGACATT GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT
    GAAGAGTGGT CCACGGACCT CAATCGGGCC GTCGATGAGC AAGGCTGGGA GTACAGTATC
    ACCATCCCCC CGGATCGGAA GCCAAAGCAC TGGGTTCCTG CTGAGAAGAT GTATTACACG
    CACCGTCGGC GGCGCTGGGT CCGCCTGCGC AGAAGGGACC TCAGCCAGAT GGAGGCATTG
    AAGAGGCACA GACAGGCAGA GGCAGAGGGT GAGGGCTGGG AGTACGCCTC GCTCTTGGGC
    TGGAAGTTCC ACCTGGAGTA CCGCAAGACT GATGCTTTCC GCCGCCGCCG CTGGCGCCGC
    CGCATGGAAC CGCTGGAGAA GACGGGGCCT GCAGCCGTGT TTGCCCTTGA GGGGGCTCTG
    GGTGGTGTGG TAGATGACAG GAGTGAAGAT TCCGTGTCCG TGTCCACCTT GAGCTTTGGA
    GTGAACAGAC CCACGATTTC CTGCATCTTT GATTATGGGA ACCGCTACCA CCTACGCTGC
    TACATGTACC AGGCTCGGGA CCTGCCCTCG ATGGACAAGG ACTCTTTTTC TGATCCCTAC
    GCCATCGTCT CCTTCCTGCA CCAGAGCCAG AAGACAGTGG TGGCGAAGAA CACTCTGAAC
    CCCACCTGGG ACCAGACGCT TATCTTCTAT GAGATTGAGA TCTTTGGTGA GCCAGCCAGT
    ATCGCTGAGC AGCCGCCCAG CATTGTGGTG GAGCTGTATG ACCACGACAC CTACGGTGCA
    GATGAGTTTA TGGGACGCTG CATTTGTCAG CCCAGCCTGG AGCGGACACC CCGGCTGGCC
    TGGTTCCCAC TGATTAGGGG CAGCCAGTCG GCGGGGGAAC TGCTGGCCTC TTTTGAGCTC
    ATCCAGAGAG AAAAGCCGGC CATTCACCAT ATTCCTGGTT TTGAGGTACA GGATATGTCA
    GGAATCCTGG AAGAGTCAGA GGACATAGAC CTGCCCTACC CGCCACCTCA GAGGGAGGCC
    AACATCTTCA TGGTCCCCCA GAACATCAAG CCTGTTCTCC AGCGTACTGC CATAGAGATC
    CTGGCGTGGG GCCTGCGGAA CATGAAGAGC TACCAGCTAG CCAGCATCTC CTCCCCTAGC
    CTTGTGGTAG AGTGCGGGGG CCAGACAGTG CAGTCCTGTG TCATCAAGAA CCTCCGGAAG
    AACCCCAACT TCGACATCTG TACCCTTTTC ATGGAAGTGA TGCTGCCCAA GGAGGAGGTC
    TACTGTCCCT CCATCGTGAT CAAGGTCATT GATAACCGCC AGTTTGGCCG CCGGCCTGTG
    GTGGGCCAGT GTACCATCCG TTCCCTGGAG AGCTTCATGT GTGACCTGTA CTCCGAGGAG
    AATCCGTCCC CACAGGGCGG CCCAGATGAT GTGAGTTTAC TCAGTCCTGG GGAAGACGTG
    CTCATTGACA TCGATGACAA GGAGCCCCTC ATCCCCATGC AGGAGGAAGA GTTCATCGAC
    TGGTGGAGCA AATTCTTTGC CTCCACAGGG GAGAGGGAAA AGTGCGGCTC ATACTTGGAG
    AAGGGCTTTG ACACTCTAAA GGTCTATGAC ACACAACTGG AGAACGTGGA GGCCTTTGAG
    GGCCTGTCTG ACTTTTGTAA CACCTTCAAG CTCTACCGGG GCAAGACTCA GGAGGAGACA
    GAGGACCCAT CTGTTATTGG GGAGTTTAAG GGCCTCTTCA AAATCTATCC CCTCCCAGAA
    GACCCGGCTG TTCCCATGCC CCCGAGACAA TTCCACCAGC TGGCTGCCCA AGGGCCTCAG
    GAGTGCCTGG TCCGCATCTA TATCATCCGA GCATTTGGCC TTCAGCCCAA GGACCCCAAT
    GGGAAGTGTG ATCCTTACAT CAAGATCTCC ATAGGGAAGA AATCAGTGAG TGACCAGGAT
    AACTACATTC CCTGTACTCT GGAGCCTGTG TTTGGAAAGA TGTTCGAGCT GACCTGCACT
    CTGCCTCTGG AGAAGGACCT GAAGATCACA CTCTATGACT ATGACCTCCT CTCCAAAGAT
    GAAAAGATTG GGGAGACAGT CATTGACCTG GAGGATAGGC TGCTGTCCAA GTTTGGGGCT
    CGCTGCGGAC TCCCACAGAC GTACTGTGTC TCTGGACCCA ACCAGTGGAG GGACCAGCTC
    CGCCCCTCCC AGCTTCTCCA CCTCTTCTGC CAGCAGCGCA GACTCAAGGC TCCTGTGTAC
    CTCACGGACC ACGTGGTGTT CCAGGATAAA GAATACAGCA TTGAAGAAAT AGAGGCTGGC
    CGAGTCCCGA ACCCACACCT GGGCCCAGTG GAAGAGCGTC TGGCCTTGCA TGTCCTCCAG
    CAGCAGGGCC TGGTCCCTGA GCATGTGGAG TCCCGGCCTC TCTACAGCCC CCTGCAGCCA
    GACATTGAAC AGGGGAAGCT GCAGATGTGG ATCGACCTAT TTCCCAAGGC CCTGGGGCGT
    CCCGGACCTC CCTTCAACAT CACCCCGCGG AGAGCCAGAA GGTTTTTCCT GCGTTGTATC
    ATCTGGAACA CAAAGGACGT GATCCTGGAC GACCTCAGCA TCACAGGGGA GAAGATGAGT
    GATATTTATG TGAAGGGTTG GATGGTTGGC TTTGAAGAGC ACAAGCAAAA GACAGATGTG
    CATTACCGGT CCCTGGGAGG TGAAGGCAAC TTTAACTGGA GGTTCATTTT CCCCTTTGAC
    TACCTGCCAG CCGAGCAAGT CTGCACCGTC TCTAAGAAGG ATGCTTTCTG GAGGCTGGAC
    AAGACTGAGA GCAAAATCCC AGCACGAGTG GTATTTCAGA TCTGGGACAA TGACAAGTTC
    TCCTTTGACG ACTTTCTGGG CTCCTTGCAG TTGGATCTCA ACCACATGCC CAAGCCTGCC
    AAGACAGCTG AGAAGTGCTC CTTGGACCAG CTGGATGACA CTTTCCACCC GGAGTGGTTT
    GTGTCCCTTT TTGAACAGAA AACAGTTAAG GGCTGGTGGC CCTGCGTAGC AGAAGAGGGC
    GAGAAGAAGA TCCTGGCAGG CAAGCTGGAA ATGACCTTGG AGATCGTAGC AGAGAGTGAG
    CATGAGGAGC GGCCTGCTGG CCAGGGCCGG GATGAACCCA ACATGAACCC CAAGCTCGAG
    GACCCAAGGC GCCCTGACAC CTCCTTCCTC TGGTTCACCT CTCCATACAA GACTATGAAG
    TTCATCCTGT GGCGGCGGTT CCGATGTGCC ATTATCCTCT TCATCATTCT CTTCATCCTG
    CTACTATTCT TAGGCATCTT CATCTATGCT TTCCCGAACT ACGCTGCCAT GAAGCTGGTG
    AAGCCCTTCA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003432275.1
    CDS51..6305
    Translation

    Target ORF information:

    RefSeq Version XM_003432227.3
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris dysferlin (DYSF), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003432227.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG ACACAGGAGG AGAGGAGGAC
    ACTGAGGACC AGGGGCTCAC TGGAGACGAG GGAGAGCCAT TACTGGATCA GAGTGGCGTC
    CCAGGCCCTG GGGCTCCCAC CACCCCGAGG AAGCCACCTT CCCATCTGCC CCCCTACCAC
    CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC GCAGCCAGAA AGCTGCTGTC CGACAAACCG
    CAGGACTTCC AGATCAGAGT GCAAGTGATC GAGGGCCGCC AGCTGCCAGG GGTGAATATC
    AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG CAGACCAAGA GGACACGGAT CCACAAGGGA
    AACAGCCCAC TATTTAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TTCGGAGCTG
    TTTGATGAGC CCATCTTTAT CACGGTCGTA GACTCCCGTT CTCTCAGGAC AGATGCTCTC
    ATTGGGGAAT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA TGCTTATCTG
    AGGAAGTGGC TGCTACTCTC CAACCCTGAT GACTTCTCTG TTGGGGCCAG AGGTTACCTG
    AAAGCAAGCC TTTATGTGCT GGGGCCCGGA GATGAAGCGC CTCTGGAGAG AAAGGACCCC
    TCTGAAGACA AGGAGGACAT TGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTGCGA
    GGAGCCCACT TCTGCCTGAA GATCTTCAGA GCGGAGGACT TACCACAAAT GGATGATGCC
    GTGATGGACA ATGTGAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA TTTGGTAGAT
    CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA
    GCCAACCCTC AGTGGAACCA GAGCATCACT CTGCCTGCCA TGTTTCCCTC CATGTGTGAA
    AAAATGAAGA TTCGCGTCAT GGACTGGGAC CGCCTTACCC ACAATGACAT CGTGGCCACC
    ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGAAGA GGAGCCTGCA
    GGTGTGGTCA AGCCTTCTCC AGCCACGGAC CTGGATGACC ATCTGGGCTT CCTTCCCACC
    TTCGGGCCCT GCTTCGTCAA TCTCTATGGC AGCCCCAGGG AATTCACTGG CTTCCCGGAC
    CCCTACGCGG AACTCAACAT GGGCAAGGGG GAAGGCGTGG CCTACCGAGG TCGGCTTCTG
    CTCTCCCTGG AGACCAAGCT GGTGGAGCAC AGCGGCGAGC AAAGGGTGGA GGACCTCCCT
    GCTGATGACA TCCTTCGGGT GGAGAAATAC CTCCGGAGGC GCAAGTACTC TCTCTTTGCC
    GCCTTCTACT CGGCCACCAT GCTCCAGGAT GTCGACGATG CCATCCAGTT TGAGGTCAGC
    ATTGGGAACT ATGGCAACAA GTTCGACACG ACCTGTCTGC CACTGGCCTC CACCACCCAG
    TACAGCCGGG CAGTCTTTGA TGGGTGCCAC TACTACTACT TGCCCTGGGG CAATGTGAAG
    CCTGTGGTGG TGCTGTCATC CTACTGGGAA GACATCAGCC ACAGAGTCGA GGCTCAGAAC
    CAGCTGCTCA GGATCGCTGA CCATCTGGAA GCTGGCCTGG AGCAAGTCCA CTTGGCTTTA
    AAGGCGCAGT TCAACACAGA GGATGTGGAC TCCCTGTTGG CCCAGCTGAT GGATGAGCTC
    ATCTCAGACT GCAGCCAGCC CCTGGGCAAT GTCCAAGAGA TACCCTCTGC CACCCACCTG
    GACCGGCACC TGTACAGGCT GCGCACCCGT CACCTAGGCC AGATTACTGA GGCGGCCCTG
    GCTCTGAAAC TCGGGCATGG CAAGCTCCCT GCCGCCCTGG AGCAGGCTGA AGACTGGCTC
    CTGCGTCTCC GTGCCCTGGC TGATGAGCCC CAGAACAGCC TGCCCGACAT CATCATCTGG
    ATGCTGCAGG GAAACAAGCG AGTGGCGTAC CAGCGGGTGC CTGCGCACGA GGTCCTCTTC
    TCCCGGCGGG GCGCCAGCTA CTGTGGCAAG AACTGTGGGA AGCTGCAGAC GATATTTCTG
    AAATATCCCA TGGAGGGGGT GCCCGGAGCC CGGATGCCAG TACAAATACG GGTGAAACTC
    TGGTTTGGGC TTTCTGTGGA TGAGAAGGAG TTCAACCAGT TTGCTGAGGG AAAGCTGTCC
    GTCTTTGCTG AAACTTATGA GAACCAGACC AAACTGGCCC TGGTTGGGAA CTGGGGCACA
    ACAGGCCTCA CCTACCCCAA GTTTTCTGAT GTCATGGGCA AGATCAAGCT ACCCAAGGAT
    AGCTTCCGCC CCTCAGCTGG CTGGACCTGG GCCGGAGACT GGTTCGTGTG TCCAGAGAAG
    ACCCTACTCC ATGACATGGA CGCGGGCCAC CTGAGTTTTG TGGAGGAGGT GTTTGAGAAC
    CAGACCCGGC TCCCTGGAGG CCAGTGGATC TACATGAGTG ACAACTACAC AGATGTGAAT
    GGGGATAAGG TGCTTCCTAA GGATGACATT GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT
    GAAGAGTGGT CCACGGACCT CAATCGGGCC GTCGATGAGC AAGGCTGGGA GTACAGTATC
    ACCATCCCCC CGGATCGGAA GCCAAAGCAC TGGGTTCCTG CTGAGAAGAT GTATTACACG
    CACCGTCGGC GGCGCTGGGT CCGCCTGCGC AGAAGGGACC TCAGCCAGAT GGAGGCATTG
    AAGAGGCACA GACAGGCAGA GGCAGAGGGT GAGGGCTGGG AGTACGCCTC GCTCTTGGGC
    TGGAAGTTCC ACCTGGAGTA CCGCAAGACT GATGCTTTCC GCCGCCGCCG CTGGCGCCGC
    CGCATGGAAC CGCTGGAGAA GACGGGGCCT GCAGCCGTGT TTGCCCTTGA GGGGGCTCTG
    GGTGGTGTGG TAGATGACAG GAGTGAAGAT TCCGTGTCCG TGTCCACCTT GAGCTTTGGA
    GTGAACAGAC CCACGATTTC CTGCATCTTT GATTATGGGA ACCGCTACCA CCTACGCTGC
    TACATGTACC AGGCTCGGGA CCTGCCCTCG ATGGACAAGG ACTCTTTTTC TGATCCCTAC
    GCCATCGTCT CCTTCCTGCA CCAGAGCCAG AAGACAGTGG TGGCGAAGAA CACTCTGAAC
    CCCACCTGGG ACCAGACGCT TATCTTCTAT GAGATTGAGA TCTTTGGTGA GCCAGCCAGT
    ATCGCTGAGC AGCCGCCCAG CATTGTGGTG GAGCTGTATG ACCACGACAC CTACGGTGCA
    GATGAGTTTA TGGGACGCTG CATTTGTCAG CCCAGCCTGG AGCGGACACC CCGGCTGGCC
    TGGTTCCCAC TGATTAGGGG CAGCCAGTCG GCGGGGGAAC TGCTGGCCTC TTTTGAGCTC
    ATCCAGAGAG AAAAGCCGGC CATTCACCAT ATTCCTGGTT TTGAGGTACA GGATATGTCA
    GGAATCCTGG AAGAGTCAGA GGACATAGAC CTGCCCTACC CGCCACCTCA GAGGGAGGCC
    AACATCTTCA TGGTCCCCCA GAACATCAAG CCTGTTCTCC AGCGTACTGC CATAGAGATC
    CTGGCGTGGG GCCTGCGGAA CATGAAGAGC TACCAGCTAG CCAGCATCTC CTCCCCTAGC
    CTTGTGGTAG AGTGCGGGGG CCAGACAGTG CAGTCCTGTG TCATCAAGAA CCTCCGGAAG
    AACCCCAACT TCGACATCTG TACCCTTTTC ATGGAAGTGA TGCTGCCCAA GGAGGAGGTC
    TACTGTCCCT CCATCGTGAT CAAGGTCATT GATAACCGCC AGTTTGGCCG CCGGCCTGTG
    GTGGGCCAGT GTACCATCCG TTCCCTGGAG AGCTTCATGT GTGACCTGTA CTCCGAGGAG
    AATCCGTCCC CACAGGGCGG CCCAGATGAT GTGAGTTTAC TCAGTCCTGG GGAAGACGTG
    CTCATTGACA TCGATGACAA GGAGCCCCTC ATCCCCATGC AGGAGGAAGA GTTCATCGAC
    TGGTGGAGCA AATTCTTTGC CTCCACAGGG GAGAGGGAAA AGTGCGGCTC ATACTTGGAG
    AAGGGCTTTG ACACTCTAAA GGTCTATGAC ACACAACTGG AGAACGTGGA GGCCTTTGAG
    GGCCTGTCTG ACTTTTGTAA CACCTTCAAG CTCTACCGGG GCAAGACTCA GGAGGAGACA
    GAGGACCCAT CTGTTATTGG GGAGTTTAAG GGCCTCTTCA AAATCTATCC CCTCCCAGAA
    GACCCGGCTG TTCCCATGCC CCCGAGACAA TTCCACCAGC TGGCTGCCCA AGGGCCTCAG
    GAGTGCCTGG TCCGCATCTA TATCATCCGA GCATTTGGCC TTCAGCCCAA GGACCCCAAT
    GGGAAGTGTG ATCCTTACAT CAAGATCTCC ATAGGGAAGA AATCAGTGAG TGACCAGGAT
    AACTACATTC CCTGTACTCT GGAGCCTGTG TTTGGAAAGA TGTTCGAGCT GACCTGCACT
    CTGCCTCTGG AGAAGGACCT GAAGATCACA CTCTATGACT ATGACCTCCT CTCCAAAGAT
    GAAAAGATTG GGGAGACAGT CATTGACCTG GAGGATAGGC TGCTGTCCAA GTTTGGGGCT
    CGCTGCGGAC TCCCACAGAC GTACTGTGTC TCTGGACCCA ACCAGTGGAG GGACCAGCTC
    CGCCCCTCCC AGCTTCTCCA CCTCTTCTGC CAGCAGCGCA GACTCAAGGC TCCTGTGTAC
    CTCACGGACC ACGTGGTGTT CCAGGATAAA GAATACAGCA TTGAAGAAAT AGAGGCTGGC
    CGAGTCCCGA ACCCACACCT GGGCCCAGTG GAAGAGCGTC TGGCCTTGCA TGTCCTCCAG
    CAGCAGGGCC TGGTCCCTGA GCATGTGGAG TCCCGGCCTC TCTACAGCCC CCTGCAGCCA
    GACATTGAAC AGGGGAAGCT GCAGATGTGG ATCGACCTAT TTCCCAAGGC CCTGGGGCGT
    CCCGGACCTC CCTTCAACAT CACCCCGCGG AGAGCCAGAA GGTTTTTCCT GCGTTGTATC
    ATCTGGAACA CAAAGGACGT GATCCTGGAC GACCTCAGCA TCACAGGGGA GAAGATGAGT
    GATATTTATG TGAAGGGTTG GATGGTTGGC TTTGAAGAGC ACAAGCAAAA GACAGATGTG
    CATTACCGGT CCCTGGGAGG TGAAGGCAAC TTTAACTGGA GGTTCATTTT CCCCTTTGAC
    TACCTGCCAG CCGAGCAAGT CTGCACCGTC TCTAAGAAGG ATGCTTTCTG GAGGCTGGAC
    AAGACTGAGA GCAAAATCCC AGCACGAGTG GTATTTCAGA TCTGGGACAA TGACAAGTTC
    TCCTTTGACG ACTTTCTGGG CTCCTTGCAG TTGGATCTCA ACCACATGCC CAAGCCTGCC
    AAGACAGCTG AGAAGTGCTC CTTGGACCAG CTGGATGACA CTTTCCACCC GGAGTGGTTT
    GTGTCCCTTT TTGAACAGAA AACAGTTAAG GGCTGGTGGC CCTGCGTAGC AGAAGAGGGC
    GAGAAGAAGA TCCTGGCAGG CAAGCTGGAA ATGACCTTGG AGATCGTAGC AGAGAGTGAG
    CATGAGGAGC GGCCTGCTGG CCAGGGCCGG GATGAACCCA ACATGAACCC CAAGCTCGAG
    GACCCAAGGC GCCCTGACAC CTCCTTCCTC TGGTTCACCT CTCCATACAA GACTATGAAG
    TTCATCCTGT GGCGGCGGTT CCGATGTGCC ATTATCCTCT TCATCATTCT CTTCATCCTG
    CTACTATTCT TAGGCATCTT CATCTATGCT TTCCCGAACT ACGCTGCCAT GAAGCTGGTG
    AAGCCCTTCA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe19216
    Clone ID Related Accession (Same CDS sequence) XM_003432227.3 , XM_003432227.4
    Accession Version XM_003432227.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6255bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product dysferlin isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003432227.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG ACACAGGAGG AGAGGAGGAC
    ACTGAGGACC AGGGGCTCAC TGGAGACGAG GGAGAGCCAT TACTGGATCA GAGTGGCGTC
    CCAGGCCCTG GGGCTCCCAC CACCCCGAGG AAGCCACCTT CCCATCTGCC CCCCTACCAC
    CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC GCAGCCAGAA AGCTGCTGTC CGACAAACCG
    CAGGACTTCC AGATCAGAGT GCAAGTGATC GAGGGCCGCC AGCTGCCAGG GGTGAATATC
    AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG CAGACCAAGA GGACACGGAT CCACAAGGGA
    AACAGCCCAC TATTTAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TTCGGAGCTG
    TTTGATGAGC CCATCTTTAT CACGGTCGTA GACTCCCGTT CTCTCAGGAC AGATGCTCTC
    ATTGGGGAAT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA TGCTTATCTG
    AGGAAGTGGC TGCTACTCTC CAACCCTGAT GACTTCTCTG TTGGGGCCAG AGGTTACCTG
    AAAGCAAGCC TTTATGTGCT GGGGCCCGGA GATGAAGCGC CTCTGGAGAG AAAGGACCCC
    TCTGAAGACA AGGAGGACAT TGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTGCGA
    GGAGCCCACT TCTGCCTGAA GATCTTCAGA GCGGAGGACT TACCACAAAT GGATGATGCC
    GTGATGGACA ATGTGAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA TTTGGTAGAT
    CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA
    GCCAACCCTC AGTGGAACCA GAGCATCACT CTGCCTGCCA TGTTTCCCTC CATGTGTGAA
    AAAATGAAGA TTCGCGTCAT GGACTGGGAC CGCCTTACCC ACAATGACAT CGTGGCCACC
    ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGAAGA GGAGCCTGCA
    GGTGTGGTCA AGCCTTCTCC AGCCACGGAC CTGGATGACC ATCTGGGCTT CCTTCCCACC
    TTCGGGCCCT GCTTCGTCAA TCTCTATGGC AGCCCCAGGG AATTCACTGG CTTCCCGGAC
    CCCTACGCGG AACTCAACAT GGGCAAGGGG GAAGGCGTGG CCTACCGAGG TCGGCTTCTG
    CTCTCCCTGG AGACCAAGCT GGTGGAGCAC AGCGGCGAGC AAAGGGTGGA GGACCTCCCT
    GCTGATGACA TCCTTCGGGT GGAGAAATAC CTCCGGAGGC GCAAGTACTC TCTCTTTGCC
    GCCTTCTACT CGGCCACCAT GCTCCAGGAT GTCGACGATG CCATCCAGTT TGAGGTCAGC
    ATTGGGAACT ATGGCAACAA GTTCGACACG ACCTGTCTGC CACTGGCCTC CACCACCCAG
    TACAGCCGGG CAGTCTTTGA TGGGTGCCAC TACTACTACT TGCCCTGGGG CAATGTGAAG
    CCTGTGGTGG TGCTGTCATC CTACTGGGAA GACATCAGCC ACAGAGTCGA GGCTCAGAAC
    CAGCTGCTCA GGATCGCTGA CCATCTGGAA GCTGGCCTGG AGCAAGTCCA CTTGGCTTTA
    AAGGCGCAGT TCAACACAGA GGATGTGGAC TCCCTGTTGG CCCAGCTGAT GGATGAGCTC
    ATCTCAGACT GCAGCCAGCC CCTGGGCAAT GTCCAAGAGA TACCCTCTGC CACCCACCTG
    GACCGGCACC TGTACAGGCT GCGCACCCGT CACCTAGGCC AGATTACTGA GGCGGCCCTG
    GCTCTGAAAC TCGGGCATGG CAAGCTCCCT GCCGCCCTGG AGCAGGCTGA AGACTGGCTC
    CTGCGTCTCC GTGCCCTGGC TGATGAGCCC CAGAACAGCC TGCCCGACAT CATCATCTGG
    ATGCTGCAGG GAAACAAGCG AGTGGCGTAC CAGCGGGTGC CTGCGCACGA GGTCCTCTTC
    TCCCGGCGGG GCGCCAGCTA CTGTGGCAAG AACTGTGGGA AGCTGCAGAC GATATTTCTG
    AAATATCCCA TGGAGGGGGT GCCCGGAGCC CGGATGCCAG TACAAATACG GGTGAAACTC
    TGGTTTGGGC TTTCTGTGGA TGAGAAGGAG TTCAACCAGT TTGCTGAGGG AAAGCTGTCC
    GTCTTTGCTG AAACTTATGA GAACCAGACC AAACTGGCCC TGGTTGGGAA CTGGGGCACA
    ACAGGCCTCA CCTACCCCAA GTTTTCTGAT GTCATGGGCA AGATCAAGCT ACCCAAGGAT
    AGCTTCCGCC CCTCAGCTGG CTGGACCTGG GCCGGAGACT GGTTCGTGTG TCCAGAGAAG
    ACCCTACTCC ATGACATGGA CGCGGGCCAC CTGAGTTTTG TGGAGGAGGT GTTTGAGAAC
    CAGACCCGGC TCCCTGGAGG CCAGTGGATC TACATGAGTG ACAACTACAC AGATGTGAAT
    GGGGATAAGG TGCTTCCTAA GGATGACATT GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT
    GAAGAGTGGT CCACGGACCT CAATCGGGCC GTCGATGAGC AAGGCTGGGA GTACAGTATC
    ACCATCCCCC CGGATCGGAA GCCAAAGCAC TGGGTTCCTG CTGAGAAGAT GTATTACACG
    CACCGTCGGC GGCGCTGGGT CCGCCTGCGC AGAAGGGACC TCAGCCAGAT GGAGGCATTG
    AAGAGGCACA GACAGGCAGA GGCAGAGGGT GAGGGCTGGG AGTACGCCTC GCTCTTGGGC
    TGGAAGTTCC ACCTGGAGTA CCGCAAGACT GATGCTTTCC GCCGCCGCCG CTGGCGCCGC
    CGCATGGAAC CGCTGGAGAA GACGGGGCCT GCAGCCGTGT TTGCCCTTGA GGGGGCTCTG
    GGTGGTGTGG TAGATGACAG GAGTGAAGAT TCCGTGTCCG TGTCCACCTT GAGCTTTGGA
    GTGAACAGAC CCACGATTTC CTGCATCTTT GATTATGGGA ACCGCTACCA CCTACGCTGC
    TACATGTACC AGGCTCGGGA CCTGCCCTCG ATGGACAAGG ACTCTTTTTC TGATCCCTAC
    GCCATCGTCT CCTTCCTGCA CCAGAGCCAG AAGACAGTGG TGGCGAAGAA CACTCTGAAC
    CCCACCTGGG ACCAGACGCT TATCTTCTAT GAGATTGAGA TCTTTGGTGA GCCAGCCAGT
    ATCGCTGAGC AGCCGCCCAG CATTGTGGTG GAGCTGTATG ACCACGACAC CTACGGTGCA
    GATGAGTTTA TGGGACGCTG CATTTGTCAG CCCAGCCTGG AGCGGACACC CCGGCTGGCC
    TGGTTCCCAC TGATTAGGGG CAGCCAGTCG GCGGGGGAAC TGCTGGCCTC TTTTGAGCTC
    ATCCAGAGAG AAAAGCCGGC CATTCACCAT ATTCCTGGTT TTGAGGTACA GGATATGTCA
    GGAATCCTGG AAGAGTCAGA GGACATAGAC CTGCCCTACC CGCCACCTCA GAGGGAGGCC
    AACATCTTCA TGGTCCCCCA GAACATCAAG CCTGTTCTCC AGCGTACTGC CATAGAGATC
    CTGGCGTGGG GCCTGCGGAA CATGAAGAGC TACCAGCTAG CCAGCATCTC CTCCCCTAGC
    CTTGTGGTAG AGTGCGGGGG CCAGACAGTG CAGTCCTGTG TCATCAAGAA CCTCCGGAAG
    AACCCCAACT TCGACATCTG TACCCTTTTC ATGGAAGTGA TGCTGCCCAA GGAGGAGGTC
    TACTGTCCCT CCATCGTGAT CAAGGTCATT GATAACCGCC AGTTTGGCCG CCGGCCTGTG
    GTGGGCCAGT GTACCATCCG TTCCCTGGAG AGCTTCATGT GTGACCTGTA CTCCGAGGAG
    AATCCGTCCC CACAGGGCGG CCCAGATGAT GTGAGTTTAC TCAGTCCTGG GGAAGACGTG
    CTCATTGACA TCGATGACAA GGAGCCCCTC ATCCCCATGC AGGAGGAAGA GTTCATCGAC
    TGGTGGAGCA AATTCTTTGC CTCCACAGGG GAGAGGGAAA AGTGCGGCTC ATACTTGGAG
    AAGGGCTTTG ACACTCTAAA GGTCTATGAC ACACAACTGG AGAACGTGGA GGCCTTTGAG
    GGCCTGTCTG ACTTTTGTAA CACCTTCAAG CTCTACCGGG GCAAGACTCA GGAGGAGACA
    GAGGACCCAT CTGTTATTGG GGAGTTTAAG GGCCTCTTCA AAATCTATCC CCTCCCAGAA
    GACCCGGCTG TTCCCATGCC CCCGAGACAA TTCCACCAGC TGGCTGCCCA AGGGCCTCAG
    GAGTGCCTGG TCCGCATCTA TATCATCCGA GCATTTGGCC TTCAGCCCAA GGACCCCAAT
    GGGAAGTGTG ATCCTTACAT CAAGATCTCC ATAGGGAAGA AATCAGTGAG TGACCAGGAT
    AACTACATTC CCTGTACTCT GGAGCCTGTG TTTGGAAAGA TGTTCGAGCT GACCTGCACT
    CTGCCTCTGG AGAAGGACCT GAAGATCACA CTCTATGACT ATGACCTCCT CTCCAAAGAT
    GAAAAGATTG GGGAGACAGT CATTGACCTG GAGGATAGGC TGCTGTCCAA GTTTGGGGCT
    CGCTGCGGAC TCCCACAGAC GTACTGTGTC TCTGGACCCA ACCAGTGGAG GGACCAGCTC
    CGCCCCTCCC AGCTTCTCCA CCTCTTCTGC CAGCAGCGCA GACTCAAGGC TCCTGTGTAC
    CTCACGGACC ACGTGGTGTT CCAGGATAAA GAATACAGCA TTGAAGAAAT AGAGGCTGGC
    CGAGTCCCGA ACCCACACCT GGGCCCAGTG GAAGAGCGTC TGGCCTTGCA TGTCCTCCAG
    CAGCAGGGCC TGGTCCCTGA GCATGTGGAG TCCCGGCCTC TCTACAGCCC CCTGCAGCCA
    GACATTGAAC AGGGGAAGCT GCAGATGTGG ATCGACCTAT TTCCCAAGGC CCTGGGGCGT
    CCCGGACCTC CCTTCAACAT CACCCCGCGG AGAGCCAGAA GGTTTTTCCT GCGTTGTATC
    ATCTGGAACA CAAAGGACGT GATCCTGGAC GACCTCAGCA TCACAGGGGA GAAGATGAGT
    GATATTTATG TGAAGGGTTG GATGGTTGGC TTTGAAGAGC ACAAGCAAAA GACAGATGTG
    CATTACCGGT CCCTGGGAGG TGAAGGCAAC TTTAACTGGA GGTTCATTTT CCCCTTTGAC
    TACCTGCCAG CCGAGCAAGT CTGCACCGTC TCTAAGAAGG ATGCTTTCTG GAGGCTGGAC
    AAGACTGAGA GCAAAATCCC AGCACGAGTG GTATTTCAGA TCTGGGACAA TGACAAGTTC
    TCCTTTGACG ACTTTCTGGG CTCCTTGCAG TTGGATCTCA ACCACATGCC CAAGCCTGCC
    AAGACAGCTG AGAAGTGCTC CTTGGACCAG CTGGATGACA CTTTCCACCC GGAGTGGTTT
    GTGTCCCTTT TTGAACAGAA AACAGTTAAG GGCTGGTGGC CCTGCGTAGC AGAAGAGGGC
    GAGAAGAAGA TCCTGGCAGG CAAGCTGGAA ATGACCTTGG AGATCGTAGC AGAGAGTGAG
    CATGAGGAGC GGCCTGCTGG CCAGGGCCGG GATGAACCCA ACATGAACCC CAAGCTCGAG
    GACCCAAGGC GCCCTGACAC CTCCTTCCTC TGGTTCACCT CTCCATACAA GACTATGAAG
    TTCATCCTGT GGCGGCGGTT CCGATGTGCC ATTATCCTCT TCATCATTCT CTTCATCCTG
    CTACTATTCT TAGGCATCTT CATCTATGCT TTCCCGAACT ACGCTGCCAT GAAGCTGGTG
    AAGCCCTTCA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003432275.1
    CDS23..6277
    Translation

    Target ORF information:

    RefSeq Version XM_003432227.4
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris dysferlin (DYSF), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003432227.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG ACACAGGAGG AGAGGAGGAC
    ACTGAGGACC AGGGGCTCAC TGGAGACGAG GGAGAGCCAT TACTGGATCA GAGTGGCGTC
    CCAGGCCCTG GGGCTCCCAC CACCCCGAGG AAGCCACCTT CCCATCTGCC CCCCTACCAC
    CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC GCAGCCAGAA AGCTGCTGTC CGACAAACCG
    CAGGACTTCC AGATCAGAGT GCAAGTGATC GAGGGCCGCC AGCTGCCAGG GGTGAATATC
    AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG CAGACCAAGA GGACACGGAT CCACAAGGGA
    AACAGCCCAC TATTTAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TTCGGAGCTG
    TTTGATGAGC CCATCTTTAT CACGGTCGTA GACTCCCGTT CTCTCAGGAC AGATGCTCTC
    ATTGGGGAAT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA TGCTTATCTG
    AGGAAGTGGC TGCTACTCTC CAACCCTGAT GACTTCTCTG TTGGGGCCAG AGGTTACCTG
    AAAGCAAGCC TTTATGTGCT GGGGCCCGGA GATGAAGCGC CTCTGGAGAG AAAGGACCCC
    TCTGAAGACA AGGAGGACAT TGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTGCGA
    GGAGCCCACT TCTGCCTGAA GATCTTCAGA GCGGAGGACT TACCACAAAT GGATGATGCC
    GTGATGGACA ATGTGAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA TTTGGTAGAT
    CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA
    GCCAACCCTC AGTGGAACCA GAGCATCACT CTGCCTGCCA TGTTTCCCTC CATGTGTGAA
    AAAATGAAGA TTCGCGTCAT GGACTGGGAC CGCCTTACCC ACAATGACAT CGTGGCCACC
    ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGAAGA GGAGCCTGCA
    GGTGTGGTCA AGCCTTCTCC AGCCACGGAC CTGGATGACC ATCTGGGCTT CCTTCCCACC
    TTCGGGCCCT GCTTCGTCAA TCTCTATGGC AGCCCCAGGG AATTCACTGG CTTCCCGGAC
    CCCTACGCGG AACTCAACAT GGGCAAGGGG GAAGGCGTGG CCTACCGAGG TCGGCTTCTG
    CTCTCCCTGG AGACCAAGCT GGTGGAGCAC AGCGGCGAGC AAAGGGTGGA GGACCTCCCT
    GCTGATGACA TCCTTCGGGT GGAGAAATAC CTCCGGAGGC GCAAGTACTC TCTCTTTGCC
    GCCTTCTACT CGGCCACCAT GCTCCAGGAT GTCGACGATG CCATCCAGTT TGAGGTCAGC
    ATTGGGAACT ATGGCAACAA GTTCGACACG ACCTGTCTGC CACTGGCCTC CACCACCCAG
    TACAGCCGGG CAGTCTTTGA TGGGTGCCAC TACTACTACT TGCCCTGGGG CAATGTGAAG
    CCTGTGGTGG TGCTGTCATC CTACTGGGAA GACATCAGCC ACAGAGTCGA GGCTCAGAAC
    CAGCTGCTCA GGATCGCTGA CCATCTGGAA GCTGGCCTGG AGCAAGTCCA CTTGGCTTTA
    AAGGCGCAGT TCAACACAGA GGATGTGGAC TCCCTGTTGG CCCAGCTGAT GGATGAGCTC
    ATCTCAGACT GCAGCCAGCC CCTGGGCAAT GTCCAAGAGA TACCCTCTGC CACCCACCTG
    GACCGGCACC TGTACAGGCT GCGCACCCGT CACCTAGGCC AGATTACTGA GGCGGCCCTG
    GCTCTGAAAC TCGGGCATGG CAAGCTCCCT GCCGCCCTGG AGCAGGCTGA AGACTGGCTC
    CTGCGTCTCC GTGCCCTGGC TGATGAGCCC CAGAACAGCC TGCCCGACAT CATCATCTGG
    ATGCTGCAGG GAAACAAGCG AGTGGCGTAC CAGCGGGTGC CTGCGCACGA GGTCCTCTTC
    TCCCGGCGGG GCGCCAGCTA CTGTGGCAAG AACTGTGGGA AGCTGCAGAC GATATTTCTG
    AAATATCCCA TGGAGGGGGT GCCCGGAGCC CGGATGCCAG TACAAATACG GGTGAAACTC
    TGGTTTGGGC TTTCTGTGGA TGAGAAGGAG TTCAACCAGT TTGCTGAGGG AAAGCTGTCC
    GTCTTTGCTG AAACTTATGA GAACCAGACC AAACTGGCCC TGGTTGGGAA CTGGGGCACA
    ACAGGCCTCA CCTACCCCAA GTTTTCTGAT GTCATGGGCA AGATCAAGCT ACCCAAGGAT
    AGCTTCCGCC CCTCAGCTGG CTGGACCTGG GCCGGAGACT GGTTCGTGTG TCCAGAGAAG
    ACCCTACTCC ATGACATGGA CGCGGGCCAC CTGAGTTTTG TGGAGGAGGT GTTTGAGAAC
    CAGACCCGGC TCCCTGGAGG CCAGTGGATC TACATGAGTG ACAACTACAC AGATGTGAAT
    GGGGATAAGG TGCTTCCTAA GGATGACATT GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT
    GAAGAGTGGT CCACGGACCT CAATCGGGCC GTCGATGAGC AAGGCTGGGA GTACAGTATC
    ACCATCCCCC CGGATCGGAA GCCAAAGCAC TGGGTTCCTG CTGAGAAGAT GTATTACACG
    CACCGTCGGC GGCGCTGGGT CCGCCTGCGC AGAAGGGACC TCAGCCAGAT GGAGGCATTG
    AAGAGGCACA GACAGGCAGA GGCAGAGGGT GAGGGCTGGG AGTACGCCTC GCTCTTGGGC
    TGGAAGTTCC ACCTGGAGTA CCGCAAGACT GATGCTTTCC GCCGCCGCCG CTGGCGCCGC
    CGCATGGAAC CGCTGGAGAA GACGGGGCCT GCAGCCGTGT TTGCCCTTGA GGGGGCTCTG
    GGTGGTGTGG TAGATGACAG GAGTGAAGAT TCCGTGTCCG TGTCCACCTT GAGCTTTGGA
    GTGAACAGAC CCACGATTTC CTGCATCTTT GATTATGGGA ACCGCTACCA CCTACGCTGC
    TACATGTACC AGGCTCGGGA CCTGCCCTCG ATGGACAAGG ACTCTTTTTC TGATCCCTAC
    GCCATCGTCT CCTTCCTGCA CCAGAGCCAG AAGACAGTGG TGGCGAAGAA CACTCTGAAC
    CCCACCTGGG ACCAGACGCT TATCTTCTAT GAGATTGAGA TCTTTGGTGA GCCAGCCAGT
    ATCGCTGAGC AGCCGCCCAG CATTGTGGTG GAGCTGTATG ACCACGACAC CTACGGTGCA
    GATGAGTTTA TGGGACGCTG CATTTGTCAG CCCAGCCTGG AGCGGACACC CCGGCTGGCC
    TGGTTCCCAC TGATTAGGGG CAGCCAGTCG GCGGGGGAAC TGCTGGCCTC TTTTGAGCTC
    ATCCAGAGAG AAAAGCCGGC CATTCACCAT ATTCCTGGTT TTGAGGTACA GGATATGTCA
    GGAATCCTGG AAGAGTCAGA GGACATAGAC CTGCCCTACC CGCCACCTCA GAGGGAGGCC
    AACATCTTCA TGGTCCCCCA GAACATCAAG CCTGTTCTCC AGCGTACTGC CATAGAGATC
    CTGGCGTGGG GCCTGCGGAA CATGAAGAGC TACCAGCTAG CCAGCATCTC CTCCCCTAGC
    CTTGTGGTAG AGTGCGGGGG CCAGACAGTG CAGTCCTGTG TCATCAAGAA CCTCCGGAAG
    AACCCCAACT TCGACATCTG TACCCTTTTC ATGGAAGTGA TGCTGCCCAA GGAGGAGGTC
    TACTGTCCCT CCATCGTGAT CAAGGTCATT GATAACCGCC AGTTTGGCCG CCGGCCTGTG
    GTGGGCCAGT GTACCATCCG TTCCCTGGAG AGCTTCATGT GTGACCTGTA CTCCGAGGAG
    AATCCGTCCC CACAGGGCGG CCCAGATGAT GTGAGTTTAC TCAGTCCTGG GGAAGACGTG
    CTCATTGACA TCGATGACAA GGAGCCCCTC ATCCCCATGC AGGAGGAAGA GTTCATCGAC
    TGGTGGAGCA AATTCTTTGC CTCCACAGGG GAGAGGGAAA AGTGCGGCTC ATACTTGGAG
    AAGGGCTTTG ACACTCTAAA GGTCTATGAC ACACAACTGG AGAACGTGGA GGCCTTTGAG
    GGCCTGTCTG ACTTTTGTAA CACCTTCAAG CTCTACCGGG GCAAGACTCA GGAGGAGACA
    GAGGACCCAT CTGTTATTGG GGAGTTTAAG GGCCTCTTCA AAATCTATCC CCTCCCAGAA
    GACCCGGCTG TTCCCATGCC CCCGAGACAA TTCCACCAGC TGGCTGCCCA AGGGCCTCAG
    GAGTGCCTGG TCCGCATCTA TATCATCCGA GCATTTGGCC TTCAGCCCAA GGACCCCAAT
    GGGAAGTGTG ATCCTTACAT CAAGATCTCC ATAGGGAAGA AATCAGTGAG TGACCAGGAT
    AACTACATTC CCTGTACTCT GGAGCCTGTG TTTGGAAAGA TGTTCGAGCT GACCTGCACT
    CTGCCTCTGG AGAAGGACCT GAAGATCACA CTCTATGACT ATGACCTCCT CTCCAAAGAT
    GAAAAGATTG GGGAGACAGT CATTGACCTG GAGGATAGGC TGCTGTCCAA GTTTGGGGCT
    CGCTGCGGAC TCCCACAGAC GTACTGTGTC TCTGGACCCA ACCAGTGGAG GGACCAGCTC
    CGCCCCTCCC AGCTTCTCCA CCTCTTCTGC CAGCAGCGCA GACTCAAGGC TCCTGTGTAC
    CTCACGGACC ACGTGGTGTT CCAGGATAAA GAATACAGCA TTGAAGAAAT AGAGGCTGGC
    CGAGTCCCGA ACCCACACCT GGGCCCAGTG GAAGAGCGTC TGGCCTTGCA TGTCCTCCAG
    CAGCAGGGCC TGGTCCCTGA GCATGTGGAG TCCCGGCCTC TCTACAGCCC CCTGCAGCCA
    GACATTGAAC AGGGGAAGCT GCAGATGTGG ATCGACCTAT TTCCCAAGGC CCTGGGGCGT
    CCCGGACCTC CCTTCAACAT CACCCCGCGG AGAGCCAGAA GGTTTTTCCT GCGTTGTATC
    ATCTGGAACA CAAAGGACGT GATCCTGGAC GACCTCAGCA TCACAGGGGA GAAGATGAGT
    GATATTTATG TGAAGGGTTG GATGGTTGGC TTTGAAGAGC ACAAGCAAAA GACAGATGTG
    CATTACCGGT CCCTGGGAGG TGAAGGCAAC TTTAACTGGA GGTTCATTTT CCCCTTTGAC
    TACCTGCCAG CCGAGCAAGT CTGCACCGTC TCTAAGAAGG ATGCTTTCTG GAGGCTGGAC
    AAGACTGAGA GCAAAATCCC AGCACGAGTG GTATTTCAGA TCTGGGACAA TGACAAGTTC
    TCCTTTGACG ACTTTCTGGG CTCCTTGCAG TTGGATCTCA ACCACATGCC CAAGCCTGCC
    AAGACAGCTG AGAAGTGCTC CTTGGACCAG CTGGATGACA CTTTCCACCC GGAGTGGTTT
    GTGTCCCTTT TTGAACAGAA AACAGTTAAG GGCTGGTGGC CCTGCGTAGC AGAAGAGGGC
    GAGAAGAAGA TCCTGGCAGG CAAGCTGGAA ATGACCTTGG AGATCGTAGC AGAGAGTGAG
    CATGAGGAGC GGCCTGCTGG CCAGGGCCGG GATGAACCCA ACATGAACCC CAAGCTCGAG
    GACCCAAGGC GCCCTGACAC CTCCTTCCTC TGGTTCACCT CTCCATACAA GACTATGAAG
    TTCATCCTGT GGCGGCGGTT CCGATGTGCC ATTATCCTCT TCATCATTCT CTTCATCCTG
    CTACTATTCT TAGGCATCTT CATCTATGCT TTCCCGAACT ACGCTGCCAT GAAGCTGGTG
    AAGCCCTTCA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe19217
    Clone ID Related Accession (Same CDS sequence) XM_540237.5 , XM_540237.6
    Accession Version XM_540237.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6252bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product dysferlin isoform X12
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_540237.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC
    CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC
    TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA
    CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGACA CAGGAGGAGA GGAGGACACT
    GAGGACCAGG GGCTCACTGG AGACGAGGGA GAGCCATTAC TGGATCAGAG TGGCGTCCCA
    GGCCCTGGGG CTCCCACCAC CCCGAGGAAG CCACCTTCCC ATCTGCCCCC CTACCACCCT
    GGGAGCAAGA GGAAAAGAAG TGCTCCCGCA GCCAGAAAGC TGCTGTCCGA CAAACCGCAG
    GACTTCCAGA TCAGAGTGCA AGTGATCGAG GGCCGCCAGC TGCCAGGGGT GAATATCAAG
    CCTGTGATCA AGGTCACTGC TGCTGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC
    AGCCCACTAT TTAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTTC GGAGCTGTTT
    GATGAGCCCA TCTTTATCAC GGTCGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT
    GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACATGC TTATCTGAGG
    AAGTGGCTGC TACTCTCCAA CCCTGATGAC TTCTCTGTTG GGGCCAGAGG TTACCTGAAA
    GCAAGCCTTT ATGTGCTGGG GCCCGGAGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT
    GAAGACAAGG AGGACATTGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTGCGAGGA
    GCCCACTTCT GCCTGAAGAT CTTCAGAGCG GAGGACTTAC CACAAATGGA TGATGCCGTG
    ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAATTT GGTAGATCCC
    TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC
    AACCCTCAGT GGAACCAGAG CATCACTCTG CCTGCCATGT TTCCCTCCAT GTGTGAAAAA
    ATGAAGATTC GCGTCATGGA CTGGGACCGC CTTACCCACA ATGACATCGT GGCCACCACC
    TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT
    GTGGTCAAGC CTTCTCCAGC CACGGACCTG GATGACCATC TGGGCTTCCT TCCCACCTTC
    GGGCCCTGCT TCGTCAATCT CTATGGCAGC CCCAGGGAAT TCACTGGCTT CCCGGACCCC
    TACGCGGAAC TCAACATGGG CAAGGGGGAA GGCGTGGCCT ACCGAGGTCG GCTTCTGCTC
    TCCCTGGAGA CCAAGCTGGT GGAGCACAGC GGCGAGCAAA GGGTGGAGGA CCTCCCTGCT
    GATGACATCC TTCGGGTGGA GAAATACCTC CGGAGGCGCA AGTACTCTCT CTTTGCCGCC
    TTCTACTCGG CCACCATGCT CCAGGATGTC GACGATGCCA TCCAGTTTGA GGTCAGCATT
    GGGAACTATG GCAACAAGTT CGACACGACC TGTCTGCCAC TGGCCTCCAC CACCCAGTAC
    AGCCGGGCAG TCTTTGATGG GTGCCACTAC TACTACTTGC CCTGGGGCAA TGTGAAGCCT
    GTGGTGGTGC TGTCATCCTA CTGGGAAGAC ATCAGCCACA GAGTCGAGGC TCAGAACCAG
    CTGCTCAGGA TCGCTGACCA TCTGGAAGCT GGCCTGGAGC AAGTCCACTT GGCTTTAAAG
    GCGCAGTTCA ACACAGAGGA TGTGGACTCC CTGTTGGCCC AGCTGATGGA TGAGCTCATC
    TCAGACTGCA GCCAGCCCCT GGGCAATGTC CAAGAGATAC CCTCTGCCAC CCACCTGGAC
    CGGCACCTGT ACAGGCTGCG CACCCGTCAC CTAGGCCAGA TTACTGAGGC GGCCCTGGCT
    CTGAAACTCG GGCATGGCAA GCTCCCTGCC GCCCTGGAGC AGGCTGAAGA CTGGCTCCTG
    CGTCTCCGTG CCCTGGCTGA TGAGCCCCAG AACAGCCTGC CCGACATCAT CATCTGGATG
    CTGCAGGGAA ACAAGCGAGT GGCGTACCAG CGGGTGCCTG CGCACGAGGT CCTCTTCTCC
    CGGCGGGGCG CCAGCTACTG TGGCAAGAAC TGTGGGAAGC TGCAGACGAT ATTTCTGAAA
    TATCCCATGG AGGGGGTGCC CGGAGCCCGG ATGCCAGTAC AAATACGGGT GAAACTCTGG
    TTTGGGCTTT CTGTGGATGA GAAGGAGTTC AACCAGTTTG CTGAGGGAAA GCTGTCCGTC
    TTTGCTGAAA CTTATGAGAA CCAGACCAAA CTGGCCCTGG TTGGGAACTG GGGCACAACA
    GGCCTCACCT ACCCCAAGTT TTCTGATGTC ATGGGCAAGA TCAAGCTACC CAAGGATAGC
    TTCCGCCCCT CAGCTGGCTG GACCTGGGCC GGAGACTGGT TCGTGTGTCC AGAGAAGACC
    CTACTCCATG ACATGGACGC GGGCCACCTG AGTTTTGTGG AGGAGGTGTT TGAGAACCAG
    ACCCGGCTCC CTGGAGGCCA GTGGATCTAC ATGAGTGACA ACTACACAGA TGTGAATGGG
    GATAAGGTGC TTCCTAAGGA TGACATTGAG TGCCCACTGG GCTGGAAGTG GGAAGATGAA
    GAGTGGTCCA CGGACCTCAA TCGGGCCGTC GATGAGCAAG GCTGGGAGTA CAGTATCACC
    ATCCCCCCGG ATCGGAAGCC AAAGCACTGG GTTCCTGCTG AGAAGATGTA TTACACGCAC
    CGTCGGCGGC GCTGGGTCCG CCTGCGCAGA AGGGACCTCA GCCAGATGGA GGCATTGAAG
    AGGCACAGAC AGGCAGAGGC AGAGGGTGAG GGCTGGGAGT ACGCCTCGCT CTTGGGCTGG
    AAGTTCCACC TGGAGTACCG CAAGACTGAT GCTTTCCGCC GCCGCCGCTG GCGCCGCCGC
    ATGGAACCGC TGGAGAAGAC GGGGCCTGCA GCCGTGTTTG CCCTTGAGGG GGCTCTGGGT
    GGTGTGGTAG ATGACAGGAG TGAAGATTCC GTGTCCGTGT CCACCTTGAG CTTTGGAGTG
    AACAGACCCA CGATTTCCTG CATCTTTGAT TATGGGAACC GCTACCACCT ACGCTGCTAC
    ATGTACCAGG CTCGGGACCT GCCCTCGATG GACAAGGACT CTTTTTCTGA TCCCTACGCC
    ATCGTCTCCT TCCTGCACCA GAGCCAGAAG ACAGTGGTGG CGAAGAACAC TCTGAACCCC
    ACCTGGGACC AGACGCTTAT CTTCTATGAG ATTGAGATCT TTGGTGAGCC AGCCAGTATC
    GCTGAGCAGC CGCCCAGCAT TGTGGTGGAG CTGTATGACC ACGACACCTA CGGTGCAGAT
    GAGTTTATGG GACGCTGCAT TTGTCAGCCC AGCCTGGAGC GGACACCCCG GCTGGCCTGG
    TTCCCACTGA TTAGGGGCAG CCAGTCGGCG GGGGAACTGC TGGCCTCTTT TGAGCTCATC
    CAGAGAGAAA AGCCGGCCAT TCACCATATT CCTGGTTTTG AGGTACAGGA TATGTCAGGA
    ATCCTGGAAG AGTCAGAGGA CATAGACCTG CCCTACCCGC CACCTCAGAG GGAGGCCAAC
    ATCTTCATGG TCCCCCAGAA CATCAAGCCT GTTCTCCAGC GTACTGCCAT AGAGATCCTG
    GCGTGGGGCC TGCGGAACAT GAAGAGCTAC CAGCTAGCCA GCATCTCCTC CCCTAGCCTT
    GTGGTAGAGT GCGGGGGCCA GACAGTGCAG TCCTGTGTCA TCAAGAACCT CCGGAAGAAC
    CCCAACTTCG ACATCTGTAC CCTTTTCATG GAAGTGATGC TGCCCAAGGA GGAGGTCTAC
    TGTCCCTCCA TCGTGATCAA GGTCATTGAT AACCGCCAGT TTGGCCGCCG GCCTGTGGTG
    GGCCAGTGTA CCATCCGTTC CCTGGAGAGC TTCATGTGTG ACCTGTACTC CGAGGAGAAT
    CCGTCCCCAC AGGGCGGCCC AGATGATGTG AGTTTACTCA GTCCTGGGGA AGACGTGCTC
    ATTGACATCG ATGACAAGGA GCCCCTCATC CCCATGCAGG AGGAAGAGTT CATCGACTGG
    TGGAGCAAAT TCTTTGCCTC CACAGGGGAG AGGGAAAAGT GCGGCTCATA CTTGGAGAAG
    GGCTTTGACA CTCTAAAGGT CTATGACACA CAACTGGAGA ACGTGGAGGC CTTTGAGGGC
    CTGTCTGACT TTTGTAACAC CTTCAAGCTC TACCGGGGCA AGACTCAGGA GGAGACAGAG
    GACCCATCTG TTATTGGGGA GTTTAAGGGC CTCTTCAAAA TCTATCCCCT CCCAGAAGAC
    CCGGCTGTTC CCATGCCCCC GAGACAATTC CACCAGCTGG CTGCCCAAGG GCCTCAGGAG
    TGCCTGGTCC GCATCTATAT CATCCGAGCA TTTGGCCTTC AGCCCAAGGA CCCCAATGGG
    AAGTGTGATC CTTACATCAA GATCTCCATA GGGAAGAAAT CAGTGAGTGA CCAGGATAAC
    TACATTCCCT GTACTCTGGA GCCTGTGTTT GGAAAGATGT TCGAGCTGAC CTGCACTCTG
    CCTCTGGAGA AGGACCTGAA GATCACACTC TATGACTATG ACCTCCTCTC CAAAGATGAA
    AAGATTGGGG AGACAGTCAT TGACCTGGAG GATAGGCTGC TGTCCAAGTT TGGGGCTCGC
    TGCGGACTCC CACAGACGTA CTGTGTCTCT GGACCCAACC AGTGGAGGGA CCAGCTCCGC
    CCCTCCCAGC TTCTCCACCT CTTCTGCCAG CAGCGCAGAC TCAAGGCTCC TGTGTACCTC
    ACGGACCACG TGGTGTTCCA GGATAAAGAA TACAGCATTG AAGAAATAGA GGCTGGCCGA
    GTCCCGAACC CACACCTGGG CCCAGTGGAA GAGCGTCTGG CCTTGCATGT CCTCCAGCAG
    CAGGGCCTGG TCCCTGAGCA TGTGGAGTCC CGGCCTCTCT ACAGCCCCCT GCAGCCAGAC
    ATTGAACAGG GGAAGCTGCA GATGTGGATC GACCTATTTC CCAAGGCCCT GGGGCGTCCC
    GGACCTCCCT TCAACATCAC CCCGCGGAGA GCCAGAAGGT TTTTCCTGCG TTGTATCATC
    TGGAACACAA AGGACGTGAT CCTGGACGAC CTCAGCATCA CAGGGGAGAA GATGAGTGAT
    ATTTATGTGA AGGGTTGGAT GGTTGGCTTT GAAGAGCACA AGCAAAAGAC AGATGTGCAT
    TACCGGTCCC TGGGAGGTGA AGGCAACTTT AACTGGAGGT TCATTTTCCC CTTTGACTAC
    CTGCCAGCCG AGCAAGTCTG CACCGTCTCT AAGAAGGATG CTTTCTGGAG GCTGGACAAG
    ACTGAGAGCA AAATCCCAGC ACGAGTGGTA TTTCAGATCT GGGACAATGA CAAGTTCTCC
    TTTGACGACT TTCTGGGCTC CTTGCAGTTG GATCTCAACC ACATGCCCAA GCCTGCCAAG
    ACAGCTGAGA AGTGCTCCTT GGACCAGCTG GATGACACTT TCCACCCGGA GTGGTTTGTG
    TCCCTTTTTG AACAGAAAAC AGTTAAGGGC TGGTGGCCCT GCGTAGCAGA AGAGGGCGAG
    AAGAAGATCC TGGCAGGCAA GCTGGAAATG ACCTTGGAGA TCGTAGCAGA GAGTGAGCAT
    GAGGAGCGGC CTGCTGGCCA GGGCCGGGAT GAACCCAACA TGAACCCCAA GCTCGAGGAC
    CCAAGGCGCC CTGACACCTC CTTCCTCTGG TTCACCTCTC CATACAAGAC TATGAAGTTC
    ATCCTGTGGC GGCGGTTCCG ATGTGCCATT ATCCTCTTCA TCATTCTCTT CATCCTGCTA
    CTATTCTTAG GCATCTTCAT CTATGCTTTC CCGAACTACG CTGCCATGAA GCTGGTGAAG
    CCCTTCAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_540237.2
    CDS438..6689
    Translation

    Target ORF information:

    RefSeq Version XM_540237.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris dysferlin (DYSF), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_540237.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC
    CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC
    TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA
    CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGACA CAGGAGGAGA GGAGGACACT
    GAGGACCAGG GGCTCACTGG AGACGAGGGA GAGCCATTAC TGGATCAGAG TGGCGTCCCA
    GGCCCTGGGG CTCCCACCAC CCCGAGGAAG CCACCTTCCC ATCTGCCCCC CTACCACCCT
    GGGAGCAAGA GGAAAAGAAG TGCTCCCGCA GCCAGAAAGC TGCTGTCCGA CAAACCGCAG
    GACTTCCAGA TCAGAGTGCA AGTGATCGAG GGCCGCCAGC TGCCAGGGGT GAATATCAAG
    CCTGTGATCA AGGTCACTGC TGCTGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC
    AGCCCACTAT TTAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTTC GGAGCTGTTT
    GATGAGCCCA TCTTTATCAC GGTCGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT
    GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACATGC TTATCTGAGG
    AAGTGGCTGC TACTCTCCAA CCCTGATGAC TTCTCTGTTG GGGCCAGAGG TTACCTGAAA
    GCAAGCCTTT ATGTGCTGGG GCCCGGAGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT
    GAAGACAAGG AGGACATTGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTGCGAGGA
    GCCCACTTCT GCCTGAAGAT CTTCAGAGCG GAGGACTTAC CACAAATGGA TGATGCCGTG
    ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAATTT GGTAGATCCC
    TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC
    AACCCTCAGT GGAACCAGAG CATCACTCTG CCTGCCATGT TTCCCTCCAT GTGTGAAAAA
    ATGAAGATTC GCGTCATGGA CTGGGACCGC CTTACCCACA ATGACATCGT GGCCACCACC
    TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT
    GTGGTCAAGC CTTCTCCAGC CACGGACCTG GATGACCATC TGGGCTTCCT TCCCACCTTC
    GGGCCCTGCT TCGTCAATCT CTATGGCAGC CCCAGGGAAT TCACTGGCTT CCCGGACCCC
    TACGCGGAAC TCAACATGGG CAAGGGGGAA GGCGTGGCCT ACCGAGGTCG GCTTCTGCTC
    TCCCTGGAGA CCAAGCTGGT GGAGCACAGC GGCGAGCAAA GGGTGGAGGA CCTCCCTGCT
    GATGACATCC TTCGGGTGGA GAAATACCTC CGGAGGCGCA AGTACTCTCT CTTTGCCGCC
    TTCTACTCGG CCACCATGCT CCAGGATGTC GACGATGCCA TCCAGTTTGA GGTCAGCATT
    GGGAACTATG GCAACAAGTT CGACACGACC TGTCTGCCAC TGGCCTCCAC CACCCAGTAC
    AGCCGGGCAG TCTTTGATGG GTGCCACTAC TACTACTTGC CCTGGGGCAA TGTGAAGCCT
    GTGGTGGTGC TGTCATCCTA CTGGGAAGAC ATCAGCCACA GAGTCGAGGC TCAGAACCAG
    CTGCTCAGGA TCGCTGACCA TCTGGAAGCT GGCCTGGAGC AAGTCCACTT GGCTTTAAAG
    GCGCAGTTCA ACACAGAGGA TGTGGACTCC CTGTTGGCCC AGCTGATGGA TGAGCTCATC
    TCAGACTGCA GCCAGCCCCT GGGCAATGTC CAAGAGATAC CCTCTGCCAC CCACCTGGAC
    CGGCACCTGT ACAGGCTGCG CACCCGTCAC CTAGGCCAGA TTACTGAGGC GGCCCTGGCT
    CTGAAACTCG GGCATGGCAA GCTCCCTGCC GCCCTGGAGC AGGCTGAAGA CTGGCTCCTG
    CGTCTCCGTG CCCTGGCTGA TGAGCCCCAG AACAGCCTGC CCGACATCAT CATCTGGATG
    CTGCAGGGAA ACAAGCGAGT GGCGTACCAG CGGGTGCCTG CGCACGAGGT CCTCTTCTCC
    CGGCGGGGCG CCAGCTACTG TGGCAAGAAC TGTGGGAAGC TGCAGACGAT ATTTCTGAAA
    TATCCCATGG AGGGGGTGCC CGGAGCCCGG ATGCCAGTAC AAATACGGGT GAAACTCTGG
    TTTGGGCTTT CTGTGGATGA GAAGGAGTTC AACCAGTTTG CTGAGGGAAA GCTGTCCGTC
    TTTGCTGAAA CTTATGAGAA CCAGACCAAA CTGGCCCTGG TTGGGAACTG GGGCACAACA
    GGCCTCACCT ACCCCAAGTT TTCTGATGTC ATGGGCAAGA TCAAGCTACC CAAGGATAGC
    TTCCGCCCCT CAGCTGGCTG GACCTGGGCC GGAGACTGGT TCGTGTGTCC AGAGAAGACC
    CTACTCCATG ACATGGACGC GGGCCACCTG AGTTTTGTGG AGGAGGTGTT TGAGAACCAG
    ACCCGGCTCC CTGGAGGCCA GTGGATCTAC ATGAGTGACA ACTACACAGA TGTGAATGGG
    GATAAGGTGC TTCCTAAGGA TGACATTGAG TGCCCACTGG GCTGGAAGTG GGAAGATGAA
    GAGTGGTCCA CGGACCTCAA TCGGGCCGTC GATGAGCAAG GCTGGGAGTA CAGTATCACC
    ATCCCCCCGG ATCGGAAGCC AAAGCACTGG GTTCCTGCTG AGAAGATGTA TTACACGCAC
    CGTCGGCGGC GCTGGGTCCG CCTGCGCAGA AGGGACCTCA GCCAGATGGA GGCATTGAAG
    AGGCACAGAC AGGCAGAGGC AGAGGGTGAG GGCTGGGAGT ACGCCTCGCT CTTGGGCTGG
    AAGTTCCACC TGGAGTACCG CAAGACTGAT GCTTTCCGCC GCCGCCGCTG GCGCCGCCGC
    ATGGAACCGC TGGAGAAGAC GGGGCCTGCA GCCGTGTTTG CCCTTGAGGG GGCTCTGGGT
    GGTGTGGTAG ATGACAGGAG TGAAGATTCC GTGTCCGTGT CCACCTTGAG CTTTGGAGTG
    AACAGACCCA CGATTTCCTG CATCTTTGAT TATGGGAACC GCTACCACCT ACGCTGCTAC
    ATGTACCAGG CTCGGGACCT GCCCTCGATG GACAAGGACT CTTTTTCTGA TCCCTACGCC
    ATCGTCTCCT TCCTGCACCA GAGCCAGAAG ACAGTGGTGG CGAAGAACAC TCTGAACCCC
    ACCTGGGACC AGACGCTTAT CTTCTATGAG ATTGAGATCT TTGGTGAGCC AGCCAGTATC
    GCTGAGCAGC CGCCCAGCAT TGTGGTGGAG CTGTATGACC ACGACACCTA CGGTGCAGAT
    GAGTTTATGG GACGCTGCAT TTGTCAGCCC AGCCTGGAGC GGACACCCCG GCTGGCCTGG
    TTCCCACTGA TTAGGGGCAG CCAGTCGGCG GGGGAACTGC TGGCCTCTTT TGAGCTCATC
    CAGAGAGAAA AGCCGGCCAT TCACCATATT CCTGGTTTTG AGGTACAGGA TATGTCAGGA
    ATCCTGGAAG AGTCAGAGGA CATAGACCTG CCCTACCCGC CACCTCAGAG GGAGGCCAAC
    ATCTTCATGG TCCCCCAGAA CATCAAGCCT GTTCTCCAGC GTACTGCCAT AGAGATCCTG
    GCGTGGGGCC TGCGGAACAT GAAGAGCTAC CAGCTAGCCA GCATCTCCTC CCCTAGCCTT
    GTGGTAGAGT GCGGGGGCCA GACAGTGCAG TCCTGTGTCA TCAAGAACCT CCGGAAGAAC
    CCCAACTTCG ACATCTGTAC CCTTTTCATG GAAGTGATGC TGCCCAAGGA GGAGGTCTAC
    TGTCCCTCCA TCGTGATCAA GGTCATTGAT AACCGCCAGT TTGGCCGCCG GCCTGTGGTG
    GGCCAGTGTA CCATCCGTTC CCTGGAGAGC TTCATGTGTG ACCTGTACTC CGAGGAGAAT
    CCGTCCCCAC AGGGCGGCCC AGATGATGTG AGTTTACTCA GTCCTGGGGA AGACGTGCTC
    ATTGACATCG ATGACAAGGA GCCCCTCATC CCCATGCAGG AGGAAGAGTT CATCGACTGG
    TGGAGCAAAT TCTTTGCCTC CACAGGGGAG AGGGAAAAGT GCGGCTCATA CTTGGAGAAG
    GGCTTTGACA CTCTAAAGGT CTATGACACA CAACTGGAGA ACGTGGAGGC CTTTGAGGGC
    CTGTCTGACT TTTGTAACAC CTTCAAGCTC TACCGGGGCA AGACTCAGGA GGAGACAGAG
    GACCCATCTG TTATTGGGGA GTTTAAGGGC CTCTTCAAAA TCTATCCCCT CCCAGAAGAC
    CCGGCTGTTC CCATGCCCCC GAGACAATTC CACCAGCTGG CTGCCCAAGG GCCTCAGGAG
    TGCCTGGTCC GCATCTATAT CATCCGAGCA TTTGGCCTTC AGCCCAAGGA CCCCAATGGG
    AAGTGTGATC CTTACATCAA GATCTCCATA GGGAAGAAAT CAGTGAGTGA CCAGGATAAC
    TACATTCCCT GTACTCTGGA GCCTGTGTTT GGAAAGATGT TCGAGCTGAC CTGCACTCTG
    CCTCTGGAGA AGGACCTGAA GATCACACTC TATGACTATG ACCTCCTCTC CAAAGATGAA
    AAGATTGGGG AGACAGTCAT TGACCTGGAG GATAGGCTGC TGTCCAAGTT TGGGGCTCGC
    TGCGGACTCC CACAGACGTA CTGTGTCTCT GGACCCAACC AGTGGAGGGA CCAGCTCCGC
    CCCTCCCAGC TTCTCCACCT CTTCTGCCAG CAGCGCAGAC TCAAGGCTCC TGTGTACCTC
    ACGGACCACG TGGTGTTCCA GGATAAAGAA TACAGCATTG AAGAAATAGA GGCTGGCCGA
    GTCCCGAACC CACACCTGGG CCCAGTGGAA GAGCGTCTGG CCTTGCATGT CCTCCAGCAG
    CAGGGCCTGG TCCCTGAGCA TGTGGAGTCC CGGCCTCTCT ACAGCCCCCT GCAGCCAGAC
    ATTGAACAGG GGAAGCTGCA GATGTGGATC GACCTATTTC CCAAGGCCCT GGGGCGTCCC
    GGACCTCCCT TCAACATCAC CCCGCGGAGA GCCAGAAGGT TTTTCCTGCG TTGTATCATC
    TGGAACACAA AGGACGTGAT CCTGGACGAC CTCAGCATCA CAGGGGAGAA GATGAGTGAT
    ATTTATGTGA AGGGTTGGAT GGTTGGCTTT GAAGAGCACA AGCAAAAGAC AGATGTGCAT
    TACCGGTCCC TGGGAGGTGA AGGCAACTTT AACTGGAGGT TCATTTTCCC CTTTGACTAC
    CTGCCAGCCG AGCAAGTCTG CACCGTCTCT AAGAAGGATG CTTTCTGGAG GCTGGACAAG
    ACTGAGAGCA AAATCCCAGC ACGAGTGGTA TTTCAGATCT GGGACAATGA CAAGTTCTCC
    TTTGACGACT TTCTGGGCTC CTTGCAGTTG GATCTCAACC ACATGCCCAA GCCTGCCAAG
    ACAGCTGAGA AGTGCTCCTT GGACCAGCTG GATGACACTT TCCACCCGGA GTGGTTTGTG
    TCCCTTTTTG AACAGAAAAC AGTTAAGGGC TGGTGGCCCT GCGTAGCAGA AGAGGGCGAG
    AAGAAGATCC TGGCAGGCAA GCTGGAAATG ACCTTGGAGA TCGTAGCAGA GAGTGAGCAT
    GAGGAGCGGC CTGCTGGCCA GGGCCGGGAT GAACCCAACA TGAACCCCAA GCTCGAGGAC
    CCAAGGCGCC CTGACACCTC CTTCCTCTGG TTCACCTCTC CATACAAGAC TATGAAGTTC
    ATCCTGTGGC GGCGGTTCCG ATGTGCCATT ATCCTCTTCA TCATTCTCTT CATCCTGCTA
    CTATTCTTAG GCATCTTCAT CTATGCTTTC CCGAACTACG CTGCCATGAA GCTGGTGAAG
    CCCTTCAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe19217
    Clone ID Related Accession (Same CDS sequence) XM_540237.5 , XM_540237.6
    Accession Version XM_540237.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6252bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product dysferlin isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_540237.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC
    CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC
    TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA
    CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGACA CAGGAGGAGA GGAGGACACT
    GAGGACCAGG GGCTCACTGG AGACGAGGGA GAGCCATTAC TGGATCAGAG TGGCGTCCCA
    GGCCCTGGGG CTCCCACCAC CCCGAGGAAG CCACCTTCCC ATCTGCCCCC CTACCACCCT
    GGGAGCAAGA GGAAAAGAAG TGCTCCCGCA GCCAGAAAGC TGCTGTCCGA CAAACCGCAG
    GACTTCCAGA TCAGAGTGCA AGTGATCGAG GGCCGCCAGC TGCCAGGGGT GAATATCAAG
    CCTGTGATCA AGGTCACTGC TGCTGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC
    AGCCCACTAT TTAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTTC GGAGCTGTTT
    GATGAGCCCA TCTTTATCAC GGTCGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT
    GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACATGC TTATCTGAGG
    AAGTGGCTGC TACTCTCCAA CCCTGATGAC TTCTCTGTTG GGGCCAGAGG TTACCTGAAA
    GCAAGCCTTT ATGTGCTGGG GCCCGGAGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT
    GAAGACAAGG AGGACATTGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTGCGAGGA
    GCCCACTTCT GCCTGAAGAT CTTCAGAGCG GAGGACTTAC CACAAATGGA TGATGCCGTG
    ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAATTT GGTAGATCCC
    TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC
    AACCCTCAGT GGAACCAGAG CATCACTCTG CCTGCCATGT TTCCCTCCAT GTGTGAAAAA
    ATGAAGATTC GCGTCATGGA CTGGGACCGC CTTACCCACA ATGACATCGT GGCCACCACC
    TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT
    GTGGTCAAGC CTTCTCCAGC CACGGACCTG GATGACCATC TGGGCTTCCT TCCCACCTTC
    GGGCCCTGCT TCGTCAATCT CTATGGCAGC CCCAGGGAAT TCACTGGCTT CCCGGACCCC
    TACGCGGAAC TCAACATGGG CAAGGGGGAA GGCGTGGCCT ACCGAGGTCG GCTTCTGCTC
    TCCCTGGAGA CCAAGCTGGT GGAGCACAGC GGCGAGCAAA GGGTGGAGGA CCTCCCTGCT
    GATGACATCC TTCGGGTGGA GAAATACCTC CGGAGGCGCA AGTACTCTCT CTTTGCCGCC
    TTCTACTCGG CCACCATGCT CCAGGATGTC GACGATGCCA TCCAGTTTGA GGTCAGCATT
    GGGAACTATG GCAACAAGTT CGACACGACC TGTCTGCCAC TGGCCTCCAC CACCCAGTAC
    AGCCGGGCAG TCTTTGATGG GTGCCACTAC TACTACTTGC CCTGGGGCAA TGTGAAGCCT
    GTGGTGGTGC TGTCATCCTA CTGGGAAGAC ATCAGCCACA GAGTCGAGGC TCAGAACCAG
    CTGCTCAGGA TCGCTGACCA TCTGGAAGCT GGCCTGGAGC AAGTCCACTT GGCTTTAAAG
    GCGCAGTTCA ACACAGAGGA TGTGGACTCC CTGTTGGCCC AGCTGATGGA TGAGCTCATC
    TCAGACTGCA GCCAGCCCCT GGGCAATGTC CAAGAGATAC CCTCTGCCAC CCACCTGGAC
    CGGCACCTGT ACAGGCTGCG CACCCGTCAC CTAGGCCAGA TTACTGAGGC GGCCCTGGCT
    CTGAAACTCG GGCATGGCAA GCTCCCTGCC GCCCTGGAGC AGGCTGAAGA CTGGCTCCTG
    CGTCTCCGTG CCCTGGCTGA TGAGCCCCAG AACAGCCTGC CCGACATCAT CATCTGGATG
    CTGCAGGGAA ACAAGCGAGT GGCGTACCAG CGGGTGCCTG CGCACGAGGT CCTCTTCTCC
    CGGCGGGGCG CCAGCTACTG TGGCAAGAAC TGTGGGAAGC TGCAGACGAT ATTTCTGAAA
    TATCCCATGG AGGGGGTGCC CGGAGCCCGG ATGCCAGTAC AAATACGGGT GAAACTCTGG
    TTTGGGCTTT CTGTGGATGA GAAGGAGTTC AACCAGTTTG CTGAGGGAAA GCTGTCCGTC
    TTTGCTGAAA CTTATGAGAA CCAGACCAAA CTGGCCCTGG TTGGGAACTG GGGCACAACA
    GGCCTCACCT ACCCCAAGTT TTCTGATGTC ATGGGCAAGA TCAAGCTACC CAAGGATAGC
    TTCCGCCCCT CAGCTGGCTG GACCTGGGCC GGAGACTGGT TCGTGTGTCC AGAGAAGACC
    CTACTCCATG ACATGGACGC GGGCCACCTG AGTTTTGTGG AGGAGGTGTT TGAGAACCAG
    ACCCGGCTCC CTGGAGGCCA GTGGATCTAC ATGAGTGACA ACTACACAGA TGTGAATGGG
    GATAAGGTGC TTCCTAAGGA TGACATTGAG TGCCCACTGG GCTGGAAGTG GGAAGATGAA
    GAGTGGTCCA CGGACCTCAA TCGGGCCGTC GATGAGCAAG GCTGGGAGTA CAGTATCACC
    ATCCCCCCGG ATCGGAAGCC AAAGCACTGG GTTCCTGCTG AGAAGATGTA TTACACGCAC
    CGTCGGCGGC GCTGGGTCCG CCTGCGCAGA AGGGACCTCA GCCAGATGGA GGCATTGAAG
    AGGCACAGAC AGGCAGAGGC AGAGGGTGAG GGCTGGGAGT ACGCCTCGCT CTTGGGCTGG
    AAGTTCCACC TGGAGTACCG CAAGACTGAT GCTTTCCGCC GCCGCCGCTG GCGCCGCCGC
    ATGGAACCGC TGGAGAAGAC GGGGCCTGCA GCCGTGTTTG CCCTTGAGGG GGCTCTGGGT
    GGTGTGGTAG ATGACAGGAG TGAAGATTCC GTGTCCGTGT CCACCTTGAG CTTTGGAGTG
    AACAGACCCA CGATTTCCTG CATCTTTGAT TATGGGAACC GCTACCACCT ACGCTGCTAC
    ATGTACCAGG CTCGGGACCT GCCCTCGATG GACAAGGACT CTTTTTCTGA TCCCTACGCC
    ATCGTCTCCT TCCTGCACCA GAGCCAGAAG ACAGTGGTGG CGAAGAACAC TCTGAACCCC
    ACCTGGGACC AGACGCTTAT CTTCTATGAG ATTGAGATCT TTGGTGAGCC AGCCAGTATC
    GCTGAGCAGC CGCCCAGCAT TGTGGTGGAG CTGTATGACC ACGACACCTA CGGTGCAGAT
    GAGTTTATGG GACGCTGCAT TTGTCAGCCC AGCCTGGAGC GGACACCCCG GCTGGCCTGG
    TTCCCACTGA TTAGGGGCAG CCAGTCGGCG GGGGAACTGC TGGCCTCTTT TGAGCTCATC
    CAGAGAGAAA AGCCGGCCAT TCACCATATT CCTGGTTTTG AGGTACAGGA TATGTCAGGA
    ATCCTGGAAG AGTCAGAGGA CATAGACCTG CCCTACCCGC CACCTCAGAG GGAGGCCAAC
    ATCTTCATGG TCCCCCAGAA CATCAAGCCT GTTCTCCAGC GTACTGCCAT AGAGATCCTG
    GCGTGGGGCC TGCGGAACAT GAAGAGCTAC CAGCTAGCCA GCATCTCCTC CCCTAGCCTT
    GTGGTAGAGT GCGGGGGCCA GACAGTGCAG TCCTGTGTCA TCAAGAACCT CCGGAAGAAC
    CCCAACTTCG ACATCTGTAC CCTTTTCATG GAAGTGATGC TGCCCAAGGA GGAGGTCTAC
    TGTCCCTCCA TCGTGATCAA GGTCATTGAT AACCGCCAGT TTGGCCGCCG GCCTGTGGTG
    GGCCAGTGTA CCATCCGTTC CCTGGAGAGC TTCATGTGTG ACCTGTACTC CGAGGAGAAT
    CCGTCCCCAC AGGGCGGCCC AGATGATGTG AGTTTACTCA GTCCTGGGGA AGACGTGCTC
    ATTGACATCG ATGACAAGGA GCCCCTCATC CCCATGCAGG AGGAAGAGTT CATCGACTGG
    TGGAGCAAAT TCTTTGCCTC CACAGGGGAG AGGGAAAAGT GCGGCTCATA CTTGGAGAAG
    GGCTTTGACA CTCTAAAGGT CTATGACACA CAACTGGAGA ACGTGGAGGC CTTTGAGGGC
    CTGTCTGACT TTTGTAACAC CTTCAAGCTC TACCGGGGCA AGACTCAGGA GGAGACAGAG
    GACCCATCTG TTATTGGGGA GTTTAAGGGC CTCTTCAAAA TCTATCCCCT CCCAGAAGAC
    CCGGCTGTTC CCATGCCCCC GAGACAATTC CACCAGCTGG CTGCCCAAGG GCCTCAGGAG
    TGCCTGGTCC GCATCTATAT CATCCGAGCA TTTGGCCTTC AGCCCAAGGA CCCCAATGGG
    AAGTGTGATC CTTACATCAA GATCTCCATA GGGAAGAAAT CAGTGAGTGA CCAGGATAAC
    TACATTCCCT GTACTCTGGA GCCTGTGTTT GGAAAGATGT TCGAGCTGAC CTGCACTCTG
    CCTCTGGAGA AGGACCTGAA GATCACACTC TATGACTATG ACCTCCTCTC CAAAGATGAA
    AAGATTGGGG AGACAGTCAT TGACCTGGAG GATAGGCTGC TGTCCAAGTT TGGGGCTCGC
    TGCGGACTCC CACAGACGTA CTGTGTCTCT GGACCCAACC AGTGGAGGGA CCAGCTCCGC
    CCCTCCCAGC TTCTCCACCT CTTCTGCCAG CAGCGCAGAC TCAAGGCTCC TGTGTACCTC
    ACGGACCACG TGGTGTTCCA GGATAAAGAA TACAGCATTG AAGAAATAGA GGCTGGCCGA
    GTCCCGAACC CACACCTGGG CCCAGTGGAA GAGCGTCTGG CCTTGCATGT CCTCCAGCAG
    CAGGGCCTGG TCCCTGAGCA TGTGGAGTCC CGGCCTCTCT ACAGCCCCCT GCAGCCAGAC
    ATTGAACAGG GGAAGCTGCA GATGTGGATC GACCTATTTC CCAAGGCCCT GGGGCGTCCC
    GGACCTCCCT TCAACATCAC CCCGCGGAGA GCCAGAAGGT TTTTCCTGCG TTGTATCATC
    TGGAACACAA AGGACGTGAT CCTGGACGAC CTCAGCATCA CAGGGGAGAA GATGAGTGAT
    ATTTATGTGA AGGGTTGGAT GGTTGGCTTT GAAGAGCACA AGCAAAAGAC AGATGTGCAT
    TACCGGTCCC TGGGAGGTGA AGGCAACTTT AACTGGAGGT TCATTTTCCC CTTTGACTAC
    CTGCCAGCCG AGCAAGTCTG CACCGTCTCT AAGAAGGATG CTTTCTGGAG GCTGGACAAG
    ACTGAGAGCA AAATCCCAGC ACGAGTGGTA TTTCAGATCT GGGACAATGA CAAGTTCTCC
    TTTGACGACT TTCTGGGCTC CTTGCAGTTG GATCTCAACC ACATGCCCAA GCCTGCCAAG
    ACAGCTGAGA AGTGCTCCTT GGACCAGCTG GATGACACTT TCCACCCGGA GTGGTTTGTG
    TCCCTTTTTG AACAGAAAAC AGTTAAGGGC TGGTGGCCCT GCGTAGCAGA AGAGGGCGAG
    AAGAAGATCC TGGCAGGCAA GCTGGAAATG ACCTTGGAGA TCGTAGCAGA GAGTGAGCAT
    GAGGAGCGGC CTGCTGGCCA GGGCCGGGAT GAACCCAACA TGAACCCCAA GCTCGAGGAC
    CCAAGGCGCC CTGACACCTC CTTCCTCTGG TTCACCTCTC CATACAAGAC TATGAAGTTC
    ATCCTGTGGC GGCGGTTCCG ATGTGCCATT ATCCTCTTCA TCATTCTCTT CATCCTGCTA
    CTATTCTTAG GCATCTTCAT CTATGCTTTC CCGAACTACG CTGCCATGAA GCTGGTGAAG
    CCCTTCAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_540237.2
    CDS405..6656
    Translation

    Target ORF information:

    RefSeq Version XM_540237.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris dysferlin (DYSF), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_540237.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC
    CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC
    TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA
    CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGACA CAGGAGGAGA GGAGGACACT
    GAGGACCAGG GGCTCACTGG AGACGAGGGA GAGCCATTAC TGGATCAGAG TGGCGTCCCA
    GGCCCTGGGG CTCCCACCAC CCCGAGGAAG CCACCTTCCC ATCTGCCCCC CTACCACCCT
    GGGAGCAAGA GGAAAAGAAG TGCTCCCGCA GCCAGAAAGC TGCTGTCCGA CAAACCGCAG
    GACTTCCAGA TCAGAGTGCA AGTGATCGAG GGCCGCCAGC TGCCAGGGGT GAATATCAAG
    CCTGTGATCA AGGTCACTGC TGCTGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC
    AGCCCACTAT TTAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTTC GGAGCTGTTT
    GATGAGCCCA TCTTTATCAC GGTCGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT
    GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACATGC TTATCTGAGG
    AAGTGGCTGC TACTCTCCAA CCCTGATGAC TTCTCTGTTG GGGCCAGAGG TTACCTGAAA
    GCAAGCCTTT ATGTGCTGGG GCCCGGAGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT
    GAAGACAAGG AGGACATTGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTGCGAGGA
    GCCCACTTCT GCCTGAAGAT CTTCAGAGCG GAGGACTTAC CACAAATGGA TGATGCCGTG
    ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAATTT GGTAGATCCC
    TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC
    AACCCTCAGT GGAACCAGAG CATCACTCTG CCTGCCATGT TTCCCTCCAT GTGTGAAAAA
    ATGAAGATTC GCGTCATGGA CTGGGACCGC CTTACCCACA ATGACATCGT GGCCACCACC
    TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT
    GTGGTCAAGC CTTCTCCAGC CACGGACCTG GATGACCATC TGGGCTTCCT TCCCACCTTC
    GGGCCCTGCT TCGTCAATCT CTATGGCAGC CCCAGGGAAT TCACTGGCTT CCCGGACCCC
    TACGCGGAAC TCAACATGGG CAAGGGGGAA GGCGTGGCCT ACCGAGGTCG GCTTCTGCTC
    TCCCTGGAGA CCAAGCTGGT GGAGCACAGC GGCGAGCAAA GGGTGGAGGA CCTCCCTGCT
    GATGACATCC TTCGGGTGGA GAAATACCTC CGGAGGCGCA AGTACTCTCT CTTTGCCGCC
    TTCTACTCGG CCACCATGCT CCAGGATGTC GACGATGCCA TCCAGTTTGA GGTCAGCATT
    GGGAACTATG GCAACAAGTT CGACACGACC TGTCTGCCAC TGGCCTCCAC CACCCAGTAC
    AGCCGGGCAG TCTTTGATGG GTGCCACTAC TACTACTTGC CCTGGGGCAA TGTGAAGCCT
    GTGGTGGTGC TGTCATCCTA CTGGGAAGAC ATCAGCCACA GAGTCGAGGC TCAGAACCAG
    CTGCTCAGGA TCGCTGACCA TCTGGAAGCT GGCCTGGAGC AAGTCCACTT GGCTTTAAAG
    GCGCAGTTCA ACACAGAGGA TGTGGACTCC CTGTTGGCCC AGCTGATGGA TGAGCTCATC
    TCAGACTGCA GCCAGCCCCT GGGCAATGTC CAAGAGATAC CCTCTGCCAC CCACCTGGAC
    CGGCACCTGT ACAGGCTGCG CACCCGTCAC CTAGGCCAGA TTACTGAGGC GGCCCTGGCT
    CTGAAACTCG GGCATGGCAA GCTCCCTGCC GCCCTGGAGC AGGCTGAAGA CTGGCTCCTG
    CGTCTCCGTG CCCTGGCTGA TGAGCCCCAG AACAGCCTGC CCGACATCAT CATCTGGATG
    CTGCAGGGAA ACAAGCGAGT GGCGTACCAG CGGGTGCCTG CGCACGAGGT CCTCTTCTCC
    CGGCGGGGCG CCAGCTACTG TGGCAAGAAC TGTGGGAAGC TGCAGACGAT ATTTCTGAAA
    TATCCCATGG AGGGGGTGCC CGGAGCCCGG ATGCCAGTAC AAATACGGGT GAAACTCTGG
    TTTGGGCTTT CTGTGGATGA GAAGGAGTTC AACCAGTTTG CTGAGGGAAA GCTGTCCGTC
    TTTGCTGAAA CTTATGAGAA CCAGACCAAA CTGGCCCTGG TTGGGAACTG GGGCACAACA
    GGCCTCACCT ACCCCAAGTT TTCTGATGTC ATGGGCAAGA TCAAGCTACC CAAGGATAGC
    TTCCGCCCCT CAGCTGGCTG GACCTGGGCC GGAGACTGGT TCGTGTGTCC AGAGAAGACC
    CTACTCCATG ACATGGACGC GGGCCACCTG AGTTTTGTGG AGGAGGTGTT TGAGAACCAG
    ACCCGGCTCC CTGGAGGCCA GTGGATCTAC ATGAGTGACA ACTACACAGA TGTGAATGGG
    GATAAGGTGC TTCCTAAGGA TGACATTGAG TGCCCACTGG GCTGGAAGTG GGAAGATGAA
    GAGTGGTCCA CGGACCTCAA TCGGGCCGTC GATGAGCAAG GCTGGGAGTA CAGTATCACC
    ATCCCCCCGG ATCGGAAGCC AAAGCACTGG GTTCCTGCTG AGAAGATGTA TTACACGCAC
    CGTCGGCGGC GCTGGGTCCG CCTGCGCAGA AGGGACCTCA GCCAGATGGA GGCATTGAAG
    AGGCACAGAC AGGCAGAGGC AGAGGGTGAG GGCTGGGAGT ACGCCTCGCT CTTGGGCTGG
    AAGTTCCACC TGGAGTACCG CAAGACTGAT GCTTTCCGCC GCCGCCGCTG GCGCCGCCGC
    ATGGAACCGC TGGAGAAGAC GGGGCCTGCA GCCGTGTTTG CCCTTGAGGG GGCTCTGGGT
    GGTGTGGTAG ATGACAGGAG TGAAGATTCC GTGTCCGTGT CCACCTTGAG CTTTGGAGTG
    AACAGACCCA CGATTTCCTG CATCTTTGAT TATGGGAACC GCTACCACCT ACGCTGCTAC
    ATGTACCAGG CTCGGGACCT GCCCTCGATG GACAAGGACT CTTTTTCTGA TCCCTACGCC
    ATCGTCTCCT TCCTGCACCA GAGCCAGAAG ACAGTGGTGG CGAAGAACAC TCTGAACCCC
    ACCTGGGACC AGACGCTTAT CTTCTATGAG ATTGAGATCT TTGGTGAGCC AGCCAGTATC
    GCTGAGCAGC CGCCCAGCAT TGTGGTGGAG CTGTATGACC ACGACACCTA CGGTGCAGAT
    GAGTTTATGG GACGCTGCAT TTGTCAGCCC AGCCTGGAGC GGACACCCCG GCTGGCCTGG
    TTCCCACTGA TTAGGGGCAG CCAGTCGGCG GGGGAACTGC TGGCCTCTTT TGAGCTCATC
    CAGAGAGAAA AGCCGGCCAT TCACCATATT CCTGGTTTTG AGGTACAGGA TATGTCAGGA
    ATCCTGGAAG AGTCAGAGGA CATAGACCTG CCCTACCCGC CACCTCAGAG GGAGGCCAAC
    ATCTTCATGG TCCCCCAGAA CATCAAGCCT GTTCTCCAGC GTACTGCCAT AGAGATCCTG
    GCGTGGGGCC TGCGGAACAT GAAGAGCTAC CAGCTAGCCA GCATCTCCTC CCCTAGCCTT
    GTGGTAGAGT GCGGGGGCCA GACAGTGCAG TCCTGTGTCA TCAAGAACCT CCGGAAGAAC
    CCCAACTTCG ACATCTGTAC CCTTTTCATG GAAGTGATGC TGCCCAAGGA GGAGGTCTAC
    TGTCCCTCCA TCGTGATCAA GGTCATTGAT AACCGCCAGT TTGGCCGCCG GCCTGTGGTG
    GGCCAGTGTA CCATCCGTTC CCTGGAGAGC TTCATGTGTG ACCTGTACTC CGAGGAGAAT
    CCGTCCCCAC AGGGCGGCCC AGATGATGTG AGTTTACTCA GTCCTGGGGA AGACGTGCTC
    ATTGACATCG ATGACAAGGA GCCCCTCATC CCCATGCAGG AGGAAGAGTT CATCGACTGG
    TGGAGCAAAT TCTTTGCCTC CACAGGGGAG AGGGAAAAGT GCGGCTCATA CTTGGAGAAG
    GGCTTTGACA CTCTAAAGGT CTATGACACA CAACTGGAGA ACGTGGAGGC CTTTGAGGGC
    CTGTCTGACT TTTGTAACAC CTTCAAGCTC TACCGGGGCA AGACTCAGGA GGAGACAGAG
    GACCCATCTG TTATTGGGGA GTTTAAGGGC CTCTTCAAAA TCTATCCCCT CCCAGAAGAC
    CCGGCTGTTC CCATGCCCCC GAGACAATTC CACCAGCTGG CTGCCCAAGG GCCTCAGGAG
    TGCCTGGTCC GCATCTATAT CATCCGAGCA TTTGGCCTTC AGCCCAAGGA CCCCAATGGG
    AAGTGTGATC CTTACATCAA GATCTCCATA GGGAAGAAAT CAGTGAGTGA CCAGGATAAC
    TACATTCCCT GTACTCTGGA GCCTGTGTTT GGAAAGATGT TCGAGCTGAC CTGCACTCTG
    CCTCTGGAGA AGGACCTGAA GATCACACTC TATGACTATG ACCTCCTCTC CAAAGATGAA
    AAGATTGGGG AGACAGTCAT TGACCTGGAG GATAGGCTGC TGTCCAAGTT TGGGGCTCGC
    TGCGGACTCC CACAGACGTA CTGTGTCTCT GGACCCAACC AGTGGAGGGA CCAGCTCCGC
    CCCTCCCAGC TTCTCCACCT CTTCTGCCAG CAGCGCAGAC TCAAGGCTCC TGTGTACCTC
    ACGGACCACG TGGTGTTCCA GGATAAAGAA TACAGCATTG AAGAAATAGA GGCTGGCCGA
    GTCCCGAACC CACACCTGGG CCCAGTGGAA GAGCGTCTGG CCTTGCATGT CCTCCAGCAG
    CAGGGCCTGG TCCCTGAGCA TGTGGAGTCC CGGCCTCTCT ACAGCCCCCT GCAGCCAGAC
    ATTGAACAGG GGAAGCTGCA GATGTGGATC GACCTATTTC CCAAGGCCCT GGGGCGTCCC
    GGACCTCCCT TCAACATCAC CCCGCGGAGA GCCAGAAGGT TTTTCCTGCG TTGTATCATC
    TGGAACACAA AGGACGTGAT CCTGGACGAC CTCAGCATCA CAGGGGAGAA GATGAGTGAT
    ATTTATGTGA AGGGTTGGAT GGTTGGCTTT GAAGAGCACA AGCAAAAGAC AGATGTGCAT
    TACCGGTCCC TGGGAGGTGA AGGCAACTTT AACTGGAGGT TCATTTTCCC CTTTGACTAC
    CTGCCAGCCG AGCAAGTCTG CACCGTCTCT AAGAAGGATG CTTTCTGGAG GCTGGACAAG
    ACTGAGAGCA AAATCCCAGC ACGAGTGGTA TTTCAGATCT GGGACAATGA CAAGTTCTCC
    TTTGACGACT TTCTGGGCTC CTTGCAGTTG GATCTCAACC ACATGCCCAA GCCTGCCAAG
    ACAGCTGAGA AGTGCTCCTT GGACCAGCTG GATGACACTT TCCACCCGGA GTGGTTTGTG
    TCCCTTTTTG AACAGAAAAC AGTTAAGGGC TGGTGGCCCT GCGTAGCAGA AGAGGGCGAG
    AAGAAGATCC TGGCAGGCAA GCTGGAAATG ACCTTGGAGA TCGTAGCAGA GAGTGAGCAT
    GAGGAGCGGC CTGCTGGCCA GGGCCGGGAT GAACCCAACA TGAACCCCAA GCTCGAGGAC
    CCAAGGCGCC CTGACACCTC CTTCCTCTGG TTCACCTCTC CATACAAGAC TATGAAGTTC
    ATCCTGTGGC GGCGGTTCCG ATGTGCCATT ATCCTCTTCA TCATTCTCTT CATCCTGCTA
    CTATTCTTAG GCATCTTCAT CTATGCTTTC CCGAACTACG CTGCCATGAA GCTGGTGAAG
    CCCTTCAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe19218
    Clone ID Related Accession (Same CDS sequence) XM_003432232.4 , XM_003432232.3
    Accession Version XM_003432232.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6213bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product dysferlin isoform X23
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003432232.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG ACACAGGAGG AGAGGAGGAC
    ACTGAGGACC AGGGGCTCAC TGGAGACGAG GGAGAGCCAT TACTGGATCA GAGTGGCGTC
    CCAGGCCCTG GGGCTCCCAC CACCCCGAGG AAGCCACCTT CCCATCTGCC CCCCTACCAC
    CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC GCAGCCAGAA AGCTGCTGTC CGACAAACCG
    CAGGACTTCC AGATCAGAGT GCAAGTGATC GAGGGCCGCC AGCTGCCAGG GGTGAATATC
    AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG CAGACCAAGA GGACACGGAT CCACAAGGGA
    AACAGCCCAC TATTTAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TTCGGAGCTG
    TTTGATGAGC CCATCTTTAT CACGGTCGTA GACTCCCGTT CTCTCAGGAC AGATGCTCTC
    ATTGGGGAAT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA TGCTTATCTG
    AGGAAGTGGC TGCTACTCTC CAACCCTGAT GACTTCTCTG TTGGGGCCAG AGGTTACCTG
    AAAGCAAGCC TTTATGTGCT GGGGCCCGGA GATGAAGCGC CTCTGGAGAG AAAGGACCCC
    TCTGAAGACA AGGAGGACAT TGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTGCGA
    GGAGCCCACT TCTGCCTGAA GATCTTCAGA GCGGAGGACT TACCACAAAT GGATGATGCC
    GTGATGGACA ATGTGAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA TTTGGTAGAT
    CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA
    GCCAACCCTC AGTGGAACCA GAGCATCACT CTGCCTGCCA TGTTTCCCTC CATGTGTGAA
    AAAATGAAGA TTCGCGTCAT GGACTGGGAC CGCCTTACCC ACAATGACAT CGTGGCCACC
    ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGAAGT GGATGACCAT
    CTGGGCTTCC TTCCCACCTT CGGGCCCTGC TTCGTCAATC TCTATGGCAG CCCCAGGGAA
    TTCACTGGCT TCCCGGACCC CTACGCGGAA CTCAACATGG GCAAGGGGGA AGGCGTGGCC
    TACCGAGGTC GGCTTCTGCT CTCCCTGGAG ACCAAGCTGG TGGAGCACAG CGGCGAGCAA
    AGGGTGGAGG ACCTCCCTGC TGATGACATC CTTCGGGTGG AGAAATACCT CCGGAGGCGC
    AAGTACTCTC TCTTTGCCGC CTTCTACTCG GCCACCATGC TCCAGGATGT CGACGATGCC
    ATCCAGTTTG AGGTCAGCAT TGGGAACTAT GGCAACAAGT TCGACACGAC CTGTCTGCCA
    CTGGCCTCCA CCACCCAGTA CAGCCGGGCA GTCTTTGATG GGTGCCACTA CTACTACTTG
    CCCTGGGGCA ATGTGAAGCC TGTGGTGGTG CTGTCATCCT ACTGGGAAGA CATCAGCCAC
    AGAGTCGAGG CTCAGAACCA GCTGCTCAGG ATCGCTGACC ATCTGGAAGC TGGCCTGGAG
    CAAGTCCACT TGGCTTTAAA GGCGCAGTTC AACACAGAGG ATGTGGACTC CCTGTTGGCC
    CAGCTGATGG ATGAGCTCAT CTCAGACTGC AGCCAGCCCC TGGGCAATGT CCAAGAGATA
    CCCTCTGCCA CCCACCTGGA CCGGCACCTG TACAGGCTGC GCACCCGTCA CCTAGGCCAG
    ATTACTGAGG CGGCCCTGGC TCTGAAACTC GGGCATGGCA AGCTCCCTGC CGCCCTGGAG
    CAGGCTGAAG ACTGGCTCCT GCGTCTCCGT GCCCTGGCTG ATGAGCCCCA GAACAGCCTG
    CCCGACATCA TCATCTGGAT GCTGCAGGGA AACAAGCGAG TGGCGTACCA GCGGGTGCCT
    GCGCACGAGG TCCTCTTCTC CCGGCGGGGC GCCAGCTACT GTGGCAAGAA CTGTGGGAAG
    CTGCAGACGA TATTTCTGAA ATATCCCATG GAGGGGGTGC CCGGAGCCCG GATGCCAGTA
    CAAATACGGG TGAAACTCTG GTTTGGGCTT TCTGTGGATG AGAAGGAGTT CAACCAGTTT
    GCTGAGGGAA AGCTGTCCGT CTTTGCTGAA ACTTATGAGA ACCAGACCAA ACTGGCCCTG
    GTTGGGAACT GGGGCACAAC AGGCCTCACC TACCCCAAGT TTTCTGATGT CATGGGCAAG
    ATCAAGCTAC CCAAGGATAG CTTCCGCCCC TCAGCTGGCT GGACCTGGGC CGGAGACTGG
    TTCGTGTGTC CAGAGAAGAC CCTACTCCAT GACATGGACG CGGGCCACCT GAGTTTTGTG
    GAGGAGGTGT TTGAGAACCA GACCCGGCTC CCTGGAGGCC AGTGGATCTA CATGAGTGAC
    AACTACACAG ATGTGAATGG GGATAAGGTG CTTCCTAAGG ATGACATTGA GTGCCCACTG
    GGCTGGAAGT GGGAAGATGA AGAGTGGTCC ACGGACCTCA ATCGGGCCGT CGATGAGCAA
    GGCTGGGAGT ACAGTATCAC CATCCCCCCG GATCGGAAGC CAAAGCACTG GGTTCCTGCT
    GAGAAGATGT ATTACACGCA CCGTCGGCGG CGCTGGGTCC GCCTGCGCAG AAGGGACCTC
    AGCCAGATGG AGGCATTGAA GAGGCACAGA CAGGCAGAGG CAGAGGGTGA GGGCTGGGAG
    TACGCCTCGC TCTTGGGCTG GAAGTTCCAC CTGGAGTACC GCAAGACTGA TGCTTTCCGC
    CGCCGCCGCT GGCGCCGCCG CATGGAACCG CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT
    GCCCTTGAGG GGGCTCTGGG TGGTGTGGTA GATGACAGGA GTGAAGATTC CGTGTCCGTG
    TCCACCTTGA GCTTTGGAGT GAACAGACCC ACGATTTCCT GCATCTTTGA TTATGGGAAC
    CGCTACCACC TACGCTGCTA CATGTACCAG GCTCGGGACC TGCCCTCGAT GGACAAGGAC
    TCTTTTTCTG ATCCCTACGC CATCGTCTCC TTCCTGCACC AGAGCCAGAA GACAGTGGTG
    GCGAAGAACA CTCTGAACCC CACCTGGGAC CAGACGCTTA TCTTCTATGA GATTGAGATC
    TTTGGTGAGC CAGCCAGTAT CGCTGAGCAG CCGCCCAGCA TTGTGGTGGA GCTGTATGAC
    CACGACACCT ACGGTGCAGA TGAGTTTATG GGACGCTGCA TTTGTCAGCC CAGCCTGGAG
    CGGACACCCC GGCTGGCCTG GTTCCCACTG ATTAGGGGCA GCCAGTCGGC GGGGGAACTG
    CTGGCCTCTT TTGAGCTCAT CCAGAGAGAA AAGCCGGCCA TTCACCATAT TCCTGGTTTT
    GAGGTACAGG ATATGTCAGG AATCCTGGAA GAGTCAGAGG ACATAGACCT GCCCTACCCG
    CCACCTCAGA GGGAGGCCAA CATCTTCATG GTCCCCCAGA ACATCAAGCC TGTTCTCCAG
    CGTACTGCCA TAGAGATCCT GGCGTGGGGC CTGCGGAACA TGAAGAGCTA CCAGCTAGCC
    AGCATCTCCT CCCCTAGCCT TGTGGTAGAG TGCGGGGGCC AGACAGTGCA GTCCTGTGTC
    ATCAAGAACC TCCGGAAGAA CCCCAACTTC GACATCTGTA CCCTTTTCAT GGAAGTGATG
    CTGCCCAAGG AGGAGGTCTA CTGTCCCTCC ATCGTGATCA AGGTCATTGA TAACCGCCAG
    TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT ACCATCCGTT CCCTGGAGAG CTTCATGTGT
    GACCTGTACT CCGAGGAGAA TCCGTCCCCA CAGGGCGGCC CAGATGATGT GAGTTTACTC
    AGTCCTGGGG AAGACGTGCT CATTGACATC GATGACAAGG AGCCCCTCAT CCCCATGCAG
    GAGGAAGAGT TCATCGACTG GTGGAGCAAA TTCTTTGCCT CCACAGGGGA GAGGGAAAAG
    TGCGGCTCAT ACTTGGAGAA GGGCTTTGAC ACTCTAAAGG TCTATGACAC ACAACTGGAG
    AACGTGGAGG CCTTTGAGGG CCTGTCTGAC TTTTGTAACA CCTTCAAGCT CTACCGGGGC
    AAGACTCAGG AGGAGACAGA GGACCCATCT GTTATTGGGG AGTTTAAGGG CCTCTTCAAA
    ATCTATCCCC TCCCAGAAGA CCCGGCTGTT CCCATGCCCC CGAGACAATT CCACCAGCTG
    GCTGCCCAAG GGCCTCAGGA GTGCCTGGTC CGCATCTATA TCATCCGAGC ATTTGGCCTT
    CAGCCCAAGG ACCCCAATGG GAAGTGTGAT CCTTACATCA AGATCTCCAT AGGGAAGAAA
    TCAGTGAGTG ACCAGGATAA CTACATTCCC TGTACTCTGG AGCCTGTGTT TGGAAAGATG
    TTCGAGCTGA CCTGCACTCT GCCTCTGGAG AAGGACCTGA AGATCACACT CTATGACTAT
    GACCTCCTCT CCAAAGATGA AAAGATTGGG GAGACAGTCA TTGACCTGGA GGATAGGCTG
    CTGTCCAAGT TTGGGGCTCG CTGCGGACTC CCACAGACGT ACTGTGTCTC TGGACCCAAC
    CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG CTTCTCCACC TCTTCTGCCA GCAGCGCAGA
    CTCAAGGCTC CTGTGTACCT CACGGACCAC GTGGTGTTCC AGGATAAAGA ATACAGCATT
    GAAGAAATAG AGGCTGGCCG AGTCCCGAAC CCACACCTGG GCCCAGTGGA AGAGCGTCTG
    GCCTTGCATG TCCTCCAGCA GCAGGGCCTG GTCCCTGAGC ATGTGGAGTC CCGGCCTCTC
    TACAGCCCCC TGCAGCCAGA CATTGAACAG GGGAAGCTGC AGATGTGGAT CGACCTATTT
    CCCAAGGCCC TGGGGCGTCC CGGACCTCCC TTCAACATCA CCCCGCGGAG AGCCAGAAGG
    TTTTTCCTGC GTTGTATCAT CTGGAACACA AAGGACGTGA TCCTGGACGA CCTCAGCATC
    ACAGGGGAGA AGATGAGTGA TATTTATGTG AAGGGTTGGA TGGTTGGCTT TGAAGAGCAC
    AAGCAAAAGA CAGATGTGCA TTACCGGTCC CTGGGAGGTG AAGGCAACTT TAACTGGAGG
    TTCATTTTCC CCTTTGACTA CCTGCCAGCC GAGCAAGTCT GCACCGTCTC TAAGAAGGAT
    GCTTTCTGGA GGCTGGACAA GACTGAGAGC AAAATCCCAG CACGAGTGGT ATTTCAGATC
    TGGGACAATG ACAAGTTCTC CTTTGACGAC TTTCTGGGCT CCTTGCAGTT GGATCTCAAC
    CACATGCCCA AGCCTGCCAA GACAGCTGAG AAGTGCTCCT TGGACCAGCT GGATGACACT
    TTCCACCCGG AGTGGTTTGT GTCCCTTTTT GAACAGAAAA CAGTTAAGGG CTGGTGGCCC
    TGCGTAGCAG AAGAGGGCGA GAAGAAGATC CTGGCAGGCA AGCTGGAAAT GACCTTGGAG
    ATCGTAGCAG AGAGTGAGCA TGAGGAGCGG CCTGCTGGCC AGGGCCGGGA TGAACCCAAC
    ATGAACCCCA AGCTCGAGGA CCCAAGGCGC CCTGACACCT CCTTCCTCTG GTTCACCTCT
    CCATACAAGA CTATGAAGTT CATCCTGTGG CGGCGGTTCC GATGTGCCAT TATCCTCTTC
    ATCATTCTCT TCATCCTGCT ACTATTCTTA GGCATCTTCA TCTATGCTTT CCCGAACTAC
    GCTGCCATGA AGCTGGTGAA GCCCTTCAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003432280.1
    CDS22..6234
    Translation

    Target ORF information:

    RefSeq Version XM_003432232.4
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris dysferlin (DYSF), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003432232.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG ACACAGGAGG AGAGGAGGAC
    ACTGAGGACC AGGGGCTCAC TGGAGACGAG GGAGAGCCAT TACTGGATCA GAGTGGCGTC
    CCAGGCCCTG GGGCTCCCAC CACCCCGAGG AAGCCACCTT CCCATCTGCC CCCCTACCAC
    CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC GCAGCCAGAA AGCTGCTGTC CGACAAACCG
    CAGGACTTCC AGATCAGAGT GCAAGTGATC GAGGGCCGCC AGCTGCCAGG GGTGAATATC
    AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG CAGACCAAGA GGACACGGAT CCACAAGGGA
    AACAGCCCAC TATTTAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TTCGGAGCTG
    TTTGATGAGC CCATCTTTAT CACGGTCGTA GACTCCCGTT CTCTCAGGAC AGATGCTCTC
    ATTGGGGAAT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA TGCTTATCTG
    AGGAAGTGGC TGCTACTCTC CAACCCTGAT GACTTCTCTG TTGGGGCCAG AGGTTACCTG
    AAAGCAAGCC TTTATGTGCT GGGGCCCGGA GATGAAGCGC CTCTGGAGAG AAAGGACCCC
    TCTGAAGACA AGGAGGACAT TGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTGCGA
    GGAGCCCACT TCTGCCTGAA GATCTTCAGA GCGGAGGACT TACCACAAAT GGATGATGCC
    GTGATGGACA ATGTGAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA TTTGGTAGAT
    CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA
    GCCAACCCTC AGTGGAACCA GAGCATCACT CTGCCTGCCA TGTTTCCCTC CATGTGTGAA
    AAAATGAAGA TTCGCGTCAT GGACTGGGAC CGCCTTACCC ACAATGACAT CGTGGCCACC
    ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGAAGT GGATGACCAT
    CTGGGCTTCC TTCCCACCTT CGGGCCCTGC TTCGTCAATC TCTATGGCAG CCCCAGGGAA
    TTCACTGGCT TCCCGGACCC CTACGCGGAA CTCAACATGG GCAAGGGGGA AGGCGTGGCC
    TACCGAGGTC GGCTTCTGCT CTCCCTGGAG ACCAAGCTGG TGGAGCACAG CGGCGAGCAA
    AGGGTGGAGG ACCTCCCTGC TGATGACATC CTTCGGGTGG AGAAATACCT CCGGAGGCGC
    AAGTACTCTC TCTTTGCCGC CTTCTACTCG GCCACCATGC TCCAGGATGT CGACGATGCC
    ATCCAGTTTG AGGTCAGCAT TGGGAACTAT GGCAACAAGT TCGACACGAC CTGTCTGCCA
    CTGGCCTCCA CCACCCAGTA CAGCCGGGCA GTCTTTGATG GGTGCCACTA CTACTACTTG
    CCCTGGGGCA ATGTGAAGCC TGTGGTGGTG CTGTCATCCT ACTGGGAAGA CATCAGCCAC
    AGAGTCGAGG CTCAGAACCA GCTGCTCAGG ATCGCTGACC ATCTGGAAGC TGGCCTGGAG
    CAAGTCCACT TGGCTTTAAA GGCGCAGTTC AACACAGAGG ATGTGGACTC CCTGTTGGCC
    CAGCTGATGG ATGAGCTCAT CTCAGACTGC AGCCAGCCCC TGGGCAATGT CCAAGAGATA
    CCCTCTGCCA CCCACCTGGA CCGGCACCTG TACAGGCTGC GCACCCGTCA CCTAGGCCAG
    ATTACTGAGG CGGCCCTGGC TCTGAAACTC GGGCATGGCA AGCTCCCTGC CGCCCTGGAG
    CAGGCTGAAG ACTGGCTCCT GCGTCTCCGT GCCCTGGCTG ATGAGCCCCA GAACAGCCTG
    CCCGACATCA TCATCTGGAT GCTGCAGGGA AACAAGCGAG TGGCGTACCA GCGGGTGCCT
    GCGCACGAGG TCCTCTTCTC CCGGCGGGGC GCCAGCTACT GTGGCAAGAA CTGTGGGAAG
    CTGCAGACGA TATTTCTGAA ATATCCCATG GAGGGGGTGC CCGGAGCCCG GATGCCAGTA
    CAAATACGGG TGAAACTCTG GTTTGGGCTT TCTGTGGATG AGAAGGAGTT CAACCAGTTT
    GCTGAGGGAA AGCTGTCCGT CTTTGCTGAA ACTTATGAGA ACCAGACCAA ACTGGCCCTG
    GTTGGGAACT GGGGCACAAC AGGCCTCACC TACCCCAAGT TTTCTGATGT CATGGGCAAG
    ATCAAGCTAC CCAAGGATAG CTTCCGCCCC TCAGCTGGCT GGACCTGGGC CGGAGACTGG
    TTCGTGTGTC CAGAGAAGAC CCTACTCCAT GACATGGACG CGGGCCACCT GAGTTTTGTG
    GAGGAGGTGT TTGAGAACCA GACCCGGCTC CCTGGAGGCC AGTGGATCTA CATGAGTGAC
    AACTACACAG ATGTGAATGG GGATAAGGTG CTTCCTAAGG ATGACATTGA GTGCCCACTG
    GGCTGGAAGT GGGAAGATGA AGAGTGGTCC ACGGACCTCA ATCGGGCCGT CGATGAGCAA
    GGCTGGGAGT ACAGTATCAC CATCCCCCCG GATCGGAAGC CAAAGCACTG GGTTCCTGCT
    GAGAAGATGT ATTACACGCA CCGTCGGCGG CGCTGGGTCC GCCTGCGCAG AAGGGACCTC
    AGCCAGATGG AGGCATTGAA GAGGCACAGA CAGGCAGAGG CAGAGGGTGA GGGCTGGGAG
    TACGCCTCGC TCTTGGGCTG GAAGTTCCAC CTGGAGTACC GCAAGACTGA TGCTTTCCGC
    CGCCGCCGCT GGCGCCGCCG CATGGAACCG CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT
    GCCCTTGAGG GGGCTCTGGG TGGTGTGGTA GATGACAGGA GTGAAGATTC CGTGTCCGTG
    TCCACCTTGA GCTTTGGAGT GAACAGACCC ACGATTTCCT GCATCTTTGA TTATGGGAAC
    CGCTACCACC TACGCTGCTA CATGTACCAG GCTCGGGACC TGCCCTCGAT GGACAAGGAC
    TCTTTTTCTG ATCCCTACGC CATCGTCTCC TTCCTGCACC AGAGCCAGAA GACAGTGGTG
    GCGAAGAACA CTCTGAACCC CACCTGGGAC CAGACGCTTA TCTTCTATGA GATTGAGATC
    TTTGGTGAGC CAGCCAGTAT CGCTGAGCAG CCGCCCAGCA TTGTGGTGGA GCTGTATGAC
    CACGACACCT ACGGTGCAGA TGAGTTTATG GGACGCTGCA TTTGTCAGCC CAGCCTGGAG
    CGGACACCCC GGCTGGCCTG GTTCCCACTG ATTAGGGGCA GCCAGTCGGC GGGGGAACTG
    CTGGCCTCTT TTGAGCTCAT CCAGAGAGAA AAGCCGGCCA TTCACCATAT TCCTGGTTTT
    GAGGTACAGG ATATGTCAGG AATCCTGGAA GAGTCAGAGG ACATAGACCT GCCCTACCCG
    CCACCTCAGA GGGAGGCCAA CATCTTCATG GTCCCCCAGA ACATCAAGCC TGTTCTCCAG
    CGTACTGCCA TAGAGATCCT GGCGTGGGGC CTGCGGAACA TGAAGAGCTA CCAGCTAGCC
    AGCATCTCCT CCCCTAGCCT TGTGGTAGAG TGCGGGGGCC AGACAGTGCA GTCCTGTGTC
    ATCAAGAACC TCCGGAAGAA CCCCAACTTC GACATCTGTA CCCTTTTCAT GGAAGTGATG
    CTGCCCAAGG AGGAGGTCTA CTGTCCCTCC ATCGTGATCA AGGTCATTGA TAACCGCCAG
    TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT ACCATCCGTT CCCTGGAGAG CTTCATGTGT
    GACCTGTACT CCGAGGAGAA TCCGTCCCCA CAGGGCGGCC CAGATGATGT GAGTTTACTC
    AGTCCTGGGG AAGACGTGCT CATTGACATC GATGACAAGG AGCCCCTCAT CCCCATGCAG
    GAGGAAGAGT TCATCGACTG GTGGAGCAAA TTCTTTGCCT CCACAGGGGA GAGGGAAAAG
    TGCGGCTCAT ACTTGGAGAA GGGCTTTGAC ACTCTAAAGG TCTATGACAC ACAACTGGAG
    AACGTGGAGG CCTTTGAGGG CCTGTCTGAC TTTTGTAACA CCTTCAAGCT CTACCGGGGC
    AAGACTCAGG AGGAGACAGA GGACCCATCT GTTATTGGGG AGTTTAAGGG CCTCTTCAAA
    ATCTATCCCC TCCCAGAAGA CCCGGCTGTT CCCATGCCCC CGAGACAATT CCACCAGCTG
    GCTGCCCAAG GGCCTCAGGA GTGCCTGGTC CGCATCTATA TCATCCGAGC ATTTGGCCTT
    CAGCCCAAGG ACCCCAATGG GAAGTGTGAT CCTTACATCA AGATCTCCAT AGGGAAGAAA
    TCAGTGAGTG ACCAGGATAA CTACATTCCC TGTACTCTGG AGCCTGTGTT TGGAAAGATG
    TTCGAGCTGA CCTGCACTCT GCCTCTGGAG AAGGACCTGA AGATCACACT CTATGACTAT
    GACCTCCTCT CCAAAGATGA AAAGATTGGG GAGACAGTCA TTGACCTGGA GGATAGGCTG
    CTGTCCAAGT TTGGGGCTCG CTGCGGACTC CCACAGACGT ACTGTGTCTC TGGACCCAAC
    CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG CTTCTCCACC TCTTCTGCCA GCAGCGCAGA
    CTCAAGGCTC CTGTGTACCT CACGGACCAC GTGGTGTTCC AGGATAAAGA ATACAGCATT
    GAAGAAATAG AGGCTGGCCG AGTCCCGAAC CCACACCTGG GCCCAGTGGA AGAGCGTCTG
    GCCTTGCATG TCCTCCAGCA GCAGGGCCTG GTCCCTGAGC ATGTGGAGTC CCGGCCTCTC
    TACAGCCCCC TGCAGCCAGA CATTGAACAG GGGAAGCTGC AGATGTGGAT CGACCTATTT
    CCCAAGGCCC TGGGGCGTCC CGGACCTCCC TTCAACATCA CCCCGCGGAG AGCCAGAAGG
    TTTTTCCTGC GTTGTATCAT CTGGAACACA AAGGACGTGA TCCTGGACGA CCTCAGCATC
    ACAGGGGAGA AGATGAGTGA TATTTATGTG AAGGGTTGGA TGGTTGGCTT TGAAGAGCAC
    AAGCAAAAGA CAGATGTGCA TTACCGGTCC CTGGGAGGTG AAGGCAACTT TAACTGGAGG
    TTCATTTTCC CCTTTGACTA CCTGCCAGCC GAGCAAGTCT GCACCGTCTC TAAGAAGGAT
    GCTTTCTGGA GGCTGGACAA GACTGAGAGC AAAATCCCAG CACGAGTGGT ATTTCAGATC
    TGGGACAATG ACAAGTTCTC CTTTGACGAC TTTCTGGGCT CCTTGCAGTT GGATCTCAAC
    CACATGCCCA AGCCTGCCAA GACAGCTGAG AAGTGCTCCT TGGACCAGCT GGATGACACT
    TTCCACCCGG AGTGGTTTGT GTCCCTTTTT GAACAGAAAA CAGTTAAGGG CTGGTGGCCC
    TGCGTAGCAG AAGAGGGCGA GAAGAAGATC CTGGCAGGCA AGCTGGAAAT GACCTTGGAG
    ATCGTAGCAG AGAGTGAGCA TGAGGAGCGG CCTGCTGGCC AGGGCCGGGA TGAACCCAAC
    ATGAACCCCA AGCTCGAGGA CCCAAGGCGC CCTGACACCT CCTTCCTCTG GTTCACCTCT
    CCATACAAGA CTATGAAGTT CATCCTGTGG CGGCGGTTCC GATGTGCCAT TATCCTCTTC
    ATCATTCTCT TCATCCTGCT ACTATTCTTA GGCATCTTCA TCTATGCTTT CCCGAACTAC
    GCTGCCATGA AGCTGGTGAA GCCCTTCAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe19218
    Clone ID Related Accession (Same CDS sequence) XM_003432232.4 , XM_003432232.3
    Accession Version XM_003432232.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6213bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product dysferlin isoform X13
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003432232.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG ACACAGGAGG AGAGGAGGAC
    ACTGAGGACC AGGGGCTCAC TGGAGACGAG GGAGAGCCAT TACTGGATCA GAGTGGCGTC
    CCAGGCCCTG GGGCTCCCAC CACCCCGAGG AAGCCACCTT CCCATCTGCC CCCCTACCAC
    CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC GCAGCCAGAA AGCTGCTGTC CGACAAACCG
    CAGGACTTCC AGATCAGAGT GCAAGTGATC GAGGGCCGCC AGCTGCCAGG GGTGAATATC
    AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG CAGACCAAGA GGACACGGAT CCACAAGGGA
    AACAGCCCAC TATTTAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TTCGGAGCTG
    TTTGATGAGC CCATCTTTAT CACGGTCGTA GACTCCCGTT CTCTCAGGAC AGATGCTCTC
    ATTGGGGAAT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA TGCTTATCTG
    AGGAAGTGGC TGCTACTCTC CAACCCTGAT GACTTCTCTG TTGGGGCCAG AGGTTACCTG
    AAAGCAAGCC TTTATGTGCT GGGGCCCGGA GATGAAGCGC CTCTGGAGAG AAAGGACCCC
    TCTGAAGACA AGGAGGACAT TGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTGCGA
    GGAGCCCACT TCTGCCTGAA GATCTTCAGA GCGGAGGACT TACCACAAAT GGATGATGCC
    GTGATGGACA ATGTGAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA TTTGGTAGAT
    CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA
    GCCAACCCTC AGTGGAACCA GAGCATCACT CTGCCTGCCA TGTTTCCCTC CATGTGTGAA
    AAAATGAAGA TTCGCGTCAT GGACTGGGAC CGCCTTACCC ACAATGACAT CGTGGCCACC
    ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGAAGT GGATGACCAT
    CTGGGCTTCC TTCCCACCTT CGGGCCCTGC TTCGTCAATC TCTATGGCAG CCCCAGGGAA
    TTCACTGGCT TCCCGGACCC CTACGCGGAA CTCAACATGG GCAAGGGGGA AGGCGTGGCC
    TACCGAGGTC GGCTTCTGCT CTCCCTGGAG ACCAAGCTGG TGGAGCACAG CGGCGAGCAA
    AGGGTGGAGG ACCTCCCTGC TGATGACATC CTTCGGGTGG AGAAATACCT CCGGAGGCGC
    AAGTACTCTC TCTTTGCCGC CTTCTACTCG GCCACCATGC TCCAGGATGT CGACGATGCC
    ATCCAGTTTG AGGTCAGCAT TGGGAACTAT GGCAACAAGT TCGACACGAC CTGTCTGCCA
    CTGGCCTCCA CCACCCAGTA CAGCCGGGCA GTCTTTGATG GGTGCCACTA CTACTACTTG
    CCCTGGGGCA ATGTGAAGCC TGTGGTGGTG CTGTCATCCT ACTGGGAAGA CATCAGCCAC
    AGAGTCGAGG CTCAGAACCA GCTGCTCAGG ATCGCTGACC ATCTGGAAGC TGGCCTGGAG
    CAAGTCCACT TGGCTTTAAA GGCGCAGTTC AACACAGAGG ATGTGGACTC CCTGTTGGCC
    CAGCTGATGG ATGAGCTCAT CTCAGACTGC AGCCAGCCCC TGGGCAATGT CCAAGAGATA
    CCCTCTGCCA CCCACCTGGA CCGGCACCTG TACAGGCTGC GCACCCGTCA CCTAGGCCAG
    ATTACTGAGG CGGCCCTGGC TCTGAAACTC GGGCATGGCA AGCTCCCTGC CGCCCTGGAG
    CAGGCTGAAG ACTGGCTCCT GCGTCTCCGT GCCCTGGCTG ATGAGCCCCA GAACAGCCTG
    CCCGACATCA TCATCTGGAT GCTGCAGGGA AACAAGCGAG TGGCGTACCA GCGGGTGCCT
    GCGCACGAGG TCCTCTTCTC CCGGCGGGGC GCCAGCTACT GTGGCAAGAA CTGTGGGAAG
    CTGCAGACGA TATTTCTGAA ATATCCCATG GAGGGGGTGC CCGGAGCCCG GATGCCAGTA
    CAAATACGGG TGAAACTCTG GTTTGGGCTT TCTGTGGATG AGAAGGAGTT CAACCAGTTT
    GCTGAGGGAA AGCTGTCCGT CTTTGCTGAA ACTTATGAGA ACCAGACCAA ACTGGCCCTG
    GTTGGGAACT GGGGCACAAC AGGCCTCACC TACCCCAAGT TTTCTGATGT CATGGGCAAG
    ATCAAGCTAC CCAAGGATAG CTTCCGCCCC TCAGCTGGCT GGACCTGGGC CGGAGACTGG
    TTCGTGTGTC CAGAGAAGAC CCTACTCCAT GACATGGACG CGGGCCACCT GAGTTTTGTG
    GAGGAGGTGT TTGAGAACCA GACCCGGCTC CCTGGAGGCC AGTGGATCTA CATGAGTGAC
    AACTACACAG ATGTGAATGG GGATAAGGTG CTTCCTAAGG ATGACATTGA GTGCCCACTG
    GGCTGGAAGT GGGAAGATGA AGAGTGGTCC ACGGACCTCA ATCGGGCCGT CGATGAGCAA
    GGCTGGGAGT ACAGTATCAC CATCCCCCCG GATCGGAAGC CAAAGCACTG GGTTCCTGCT
    GAGAAGATGT ATTACACGCA CCGTCGGCGG CGCTGGGTCC GCCTGCGCAG AAGGGACCTC
    AGCCAGATGG AGGCATTGAA GAGGCACAGA CAGGCAGAGG CAGAGGGTGA GGGCTGGGAG
    TACGCCTCGC TCTTGGGCTG GAAGTTCCAC CTGGAGTACC GCAAGACTGA TGCTTTCCGC
    CGCCGCCGCT GGCGCCGCCG CATGGAACCG CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT
    GCCCTTGAGG GGGCTCTGGG TGGTGTGGTA GATGACAGGA GTGAAGATTC CGTGTCCGTG
    TCCACCTTGA GCTTTGGAGT GAACAGACCC ACGATTTCCT GCATCTTTGA TTATGGGAAC
    CGCTACCACC TACGCTGCTA CATGTACCAG GCTCGGGACC TGCCCTCGAT GGACAAGGAC
    TCTTTTTCTG ATCCCTACGC CATCGTCTCC TTCCTGCACC AGAGCCAGAA GACAGTGGTG
    GCGAAGAACA CTCTGAACCC CACCTGGGAC CAGACGCTTA TCTTCTATGA GATTGAGATC
    TTTGGTGAGC CAGCCAGTAT CGCTGAGCAG CCGCCCAGCA TTGTGGTGGA GCTGTATGAC
    CACGACACCT ACGGTGCAGA TGAGTTTATG GGACGCTGCA TTTGTCAGCC CAGCCTGGAG
    CGGACACCCC GGCTGGCCTG GTTCCCACTG ATTAGGGGCA GCCAGTCGGC GGGGGAACTG
    CTGGCCTCTT TTGAGCTCAT CCAGAGAGAA AAGCCGGCCA TTCACCATAT TCCTGGTTTT
    GAGGTACAGG ATATGTCAGG AATCCTGGAA GAGTCAGAGG ACATAGACCT GCCCTACCCG
    CCACCTCAGA GGGAGGCCAA CATCTTCATG GTCCCCCAGA ACATCAAGCC TGTTCTCCAG
    CGTACTGCCA TAGAGATCCT GGCGTGGGGC CTGCGGAACA TGAAGAGCTA CCAGCTAGCC
    AGCATCTCCT CCCCTAGCCT TGTGGTAGAG TGCGGGGGCC AGACAGTGCA GTCCTGTGTC
    ATCAAGAACC TCCGGAAGAA CCCCAACTTC GACATCTGTA CCCTTTTCAT GGAAGTGATG
    CTGCCCAAGG AGGAGGTCTA CTGTCCCTCC ATCGTGATCA AGGTCATTGA TAACCGCCAG
    TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT ACCATCCGTT CCCTGGAGAG CTTCATGTGT
    GACCTGTACT CCGAGGAGAA TCCGTCCCCA CAGGGCGGCC CAGATGATGT GAGTTTACTC
    AGTCCTGGGG AAGACGTGCT CATTGACATC GATGACAAGG AGCCCCTCAT CCCCATGCAG
    GAGGAAGAGT TCATCGACTG GTGGAGCAAA TTCTTTGCCT CCACAGGGGA GAGGGAAAAG
    TGCGGCTCAT ACTTGGAGAA GGGCTTTGAC ACTCTAAAGG TCTATGACAC ACAACTGGAG
    AACGTGGAGG CCTTTGAGGG CCTGTCTGAC TTTTGTAACA CCTTCAAGCT CTACCGGGGC
    AAGACTCAGG AGGAGACAGA GGACCCATCT GTTATTGGGG AGTTTAAGGG CCTCTTCAAA
    ATCTATCCCC TCCCAGAAGA CCCGGCTGTT CCCATGCCCC CGAGACAATT CCACCAGCTG
    GCTGCCCAAG GGCCTCAGGA GTGCCTGGTC CGCATCTATA TCATCCGAGC ATTTGGCCTT
    CAGCCCAAGG ACCCCAATGG GAAGTGTGAT CCTTACATCA AGATCTCCAT AGGGAAGAAA
    TCAGTGAGTG ACCAGGATAA CTACATTCCC TGTACTCTGG AGCCTGTGTT TGGAAAGATG
    TTCGAGCTGA CCTGCACTCT GCCTCTGGAG AAGGACCTGA AGATCACACT CTATGACTAT
    GACCTCCTCT CCAAAGATGA AAAGATTGGG GAGACAGTCA TTGACCTGGA GGATAGGCTG
    CTGTCCAAGT TTGGGGCTCG CTGCGGACTC CCACAGACGT ACTGTGTCTC TGGACCCAAC
    CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG CTTCTCCACC TCTTCTGCCA GCAGCGCAGA
    CTCAAGGCTC CTGTGTACCT CACGGACCAC GTGGTGTTCC AGGATAAAGA ATACAGCATT
    GAAGAAATAG AGGCTGGCCG AGTCCCGAAC CCACACCTGG GCCCAGTGGA AGAGCGTCTG
    GCCTTGCATG TCCTCCAGCA GCAGGGCCTG GTCCCTGAGC ATGTGGAGTC CCGGCCTCTC
    TACAGCCCCC TGCAGCCAGA CATTGAACAG GGGAAGCTGC AGATGTGGAT CGACCTATTT
    CCCAAGGCCC TGGGGCGTCC CGGACCTCCC TTCAACATCA CCCCGCGGAG AGCCAGAAGG
    TTTTTCCTGC GTTGTATCAT CTGGAACACA AAGGACGTGA TCCTGGACGA CCTCAGCATC
    ACAGGGGAGA AGATGAGTGA TATTTATGTG AAGGGTTGGA TGGTTGGCTT TGAAGAGCAC
    AAGCAAAAGA CAGATGTGCA TTACCGGTCC CTGGGAGGTG AAGGCAACTT TAACTGGAGG
    TTCATTTTCC CCTTTGACTA CCTGCCAGCC GAGCAAGTCT GCACCGTCTC TAAGAAGGAT
    GCTTTCTGGA GGCTGGACAA GACTGAGAGC AAAATCCCAG CACGAGTGGT ATTTCAGATC
    TGGGACAATG ACAAGTTCTC CTTTGACGAC TTTCTGGGCT CCTTGCAGTT GGATCTCAAC
    CACATGCCCA AGCCTGCCAA GACAGCTGAG AAGTGCTCCT TGGACCAGCT GGATGACACT
    TTCCACCCGG AGTGGTTTGT GTCCCTTTTT GAACAGAAAA CAGTTAAGGG CTGGTGGCCC
    TGCGTAGCAG AAGAGGGCGA GAAGAAGATC CTGGCAGGCA AGCTGGAAAT GACCTTGGAG
    ATCGTAGCAG AGAGTGAGCA TGAGGAGCGG CCTGCTGGCC AGGGCCGGGA TGAACCCAAC
    ATGAACCCCA AGCTCGAGGA CCCAAGGCGC CCTGACACCT CCTTCCTCTG GTTCACCTCT
    CCATACAAGA CTATGAAGTT CATCCTGTGG CGGCGGTTCC GATGTGCCAT TATCCTCTTC
    ATCATTCTCT TCATCCTGCT ACTATTCTTA GGCATCTTCA TCTATGCTTT CCCGAACTAC
    GCTGCCATGA AGCTGGTGAA GCCCTTCAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003432280.1
    CDS50..6262
    Translation

    Target ORF information:

    RefSeq Version XM_003432232.3
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris dysferlin (DYSF), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003432232.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
    AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG
    AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG
    GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG
    TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC
    AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG
    CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG
    GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG ACACAGGAGG AGAGGAGGAC
    ACTGAGGACC AGGGGCTCAC TGGAGACGAG GGAGAGCCAT TACTGGATCA GAGTGGCGTC
    CCAGGCCCTG GGGCTCCCAC CACCCCGAGG AAGCCACCTT CCCATCTGCC CCCCTACCAC
    CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC GCAGCCAGAA AGCTGCTGTC CGACAAACCG
    CAGGACTTCC AGATCAGAGT GCAAGTGATC GAGGGCCGCC AGCTGCCAGG GGTGAATATC
    AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG CAGACCAAGA GGACACGGAT CCACAAGGGA
    AACAGCCCAC TATTTAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TTCGGAGCTG
    TTTGATGAGC CCATCTTTAT CACGGTCGTA GACTCCCGTT CTCTCAGGAC AGATGCTCTC
    ATTGGGGAAT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA TGCTTATCTG
    AGGAAGTGGC TGCTACTCTC CAACCCTGAT GACTTCTCTG TTGGGGCCAG AGGTTACCTG
    AAAGCAAGCC TTTATGTGCT GGGGCCCGGA GATGAAGCGC CTCTGGAGAG AAAGGACCCC
    TCTGAAGACA AGGAGGACAT TGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTGCGA
    GGAGCCCACT TCTGCCTGAA GATCTTCAGA GCGGAGGACT TACCACAAAT GGATGATGCC
    GTGATGGACA ATGTGAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA TTTGGTAGAT
    CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA
    GCCAACCCTC AGTGGAACCA GAGCATCACT CTGCCTGCCA TGTTTCCCTC CATGTGTGAA
    AAAATGAAGA TTCGCGTCAT GGACTGGGAC CGCCTTACCC ACAATGACAT CGTGGCCACC
    ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGAAGT GGATGACCAT
    CTGGGCTTCC TTCCCACCTT CGGGCCCTGC TTCGTCAATC TCTATGGCAG CCCCAGGGAA
    TTCACTGGCT TCCCGGACCC CTACGCGGAA CTCAACATGG GCAAGGGGGA AGGCGTGGCC
    TACCGAGGTC GGCTTCTGCT CTCCCTGGAG ACCAAGCTGG TGGAGCACAG CGGCGAGCAA
    AGGGTGGAGG ACCTCCCTGC TGATGACATC CTTCGGGTGG AGAAATACCT CCGGAGGCGC
    AAGTACTCTC TCTTTGCCGC CTTCTACTCG GCCACCATGC TCCAGGATGT CGACGATGCC
    ATCCAGTTTG AGGTCAGCAT TGGGAACTAT GGCAACAAGT TCGACACGAC CTGTCTGCCA
    CTGGCCTCCA CCACCCAGTA CAGCCGGGCA GTCTTTGATG GGTGCCACTA CTACTACTTG
    CCCTGGGGCA ATGTGAAGCC TGTGGTGGTG CTGTCATCCT ACTGGGAAGA CATCAGCCAC
    AGAGTCGAGG CTCAGAACCA GCTGCTCAGG ATCGCTGACC ATCTGGAAGC TGGCCTGGAG
    CAAGTCCACT TGGCTTTAAA GGCGCAGTTC AACACAGAGG ATGTGGACTC CCTGTTGGCC
    CAGCTGATGG ATGAGCTCAT CTCAGACTGC AGCCAGCCCC TGGGCAATGT CCAAGAGATA
    CCCTCTGCCA CCCACCTGGA CCGGCACCTG TACAGGCTGC GCACCCGTCA CCTAGGCCAG
    ATTACTGAGG CGGCCCTGGC TCTGAAACTC GGGCATGGCA AGCTCCCTGC CGCCCTGGAG
    CAGGCTGAAG ACTGGCTCCT GCGTCTCCGT GCCCTGGCTG ATGAGCCCCA GAACAGCCTG
    CCCGACATCA TCATCTGGAT GCTGCAGGGA AACAAGCGAG TGGCGTACCA GCGGGTGCCT
    GCGCACGAGG TCCTCTTCTC CCGGCGGGGC GCCAGCTACT GTGGCAAGAA CTGTGGGAAG
    CTGCAGACGA TATTTCTGAA ATATCCCATG GAGGGGGTGC CCGGAGCCCG GATGCCAGTA
    CAAATACGGG TGAAACTCTG GTTTGGGCTT TCTGTGGATG AGAAGGAGTT CAACCAGTTT
    GCTGAGGGAA AGCTGTCCGT CTTTGCTGAA ACTTATGAGA ACCAGACCAA ACTGGCCCTG
    GTTGGGAACT GGGGCACAAC AGGCCTCACC TACCCCAAGT TTTCTGATGT CATGGGCAAG
    ATCAAGCTAC CCAAGGATAG CTTCCGCCCC TCAGCTGGCT GGACCTGGGC CGGAGACTGG
    TTCGTGTGTC CAGAGAAGAC CCTACTCCAT GACATGGACG CGGGCCACCT GAGTTTTGTG
    GAGGAGGTGT TTGAGAACCA GACCCGGCTC CCTGGAGGCC AGTGGATCTA CATGAGTGAC
    AACTACACAG ATGTGAATGG GGATAAGGTG CTTCCTAAGG ATGACATTGA GTGCCCACTG
    GGCTGGAAGT GGGAAGATGA AGAGTGGTCC ACGGACCTCA ATCGGGCCGT CGATGAGCAA
    GGCTGGGAGT ACAGTATCAC CATCCCCCCG GATCGGAAGC CAAAGCACTG GGTTCCTGCT
    GAGAAGATGT ATTACACGCA CCGTCGGCGG CGCTGGGTCC GCCTGCGCAG AAGGGACCTC
    AGCCAGATGG AGGCATTGAA GAGGCACAGA CAGGCAGAGG CAGAGGGTGA GGGCTGGGAG
    TACGCCTCGC TCTTGGGCTG GAAGTTCCAC CTGGAGTACC GCAAGACTGA TGCTTTCCGC
    CGCCGCCGCT GGCGCCGCCG CATGGAACCG CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT
    GCCCTTGAGG GGGCTCTGGG TGGTGTGGTA GATGACAGGA GTGAAGATTC CGTGTCCGTG
    TCCACCTTGA GCTTTGGAGT GAACAGACCC ACGATTTCCT GCATCTTTGA TTATGGGAAC
    CGCTACCACC TACGCTGCTA CATGTACCAG GCTCGGGACC TGCCCTCGAT GGACAAGGAC
    TCTTTTTCTG ATCCCTACGC CATCGTCTCC TTCCTGCACC AGAGCCAGAA GACAGTGGTG
    GCGAAGAACA CTCTGAACCC CACCTGGGAC CAGACGCTTA TCTTCTATGA GATTGAGATC
    TTTGGTGAGC CAGCCAGTAT CGCTGAGCAG CCGCCCAGCA TTGTGGTGGA GCTGTATGAC
    CACGACACCT ACGGTGCAGA TGAGTTTATG GGACGCTGCA TTTGTCAGCC CAGCCTGGAG
    CGGACACCCC GGCTGGCCTG GTTCCCACTG ATTAGGGGCA GCCAGTCGGC GGGGGAACTG
    CTGGCCTCTT TTGAGCTCAT CCAGAGAGAA AAGCCGGCCA TTCACCATAT TCCTGGTTTT
    GAGGTACAGG ATATGTCAGG AATCCTGGAA GAGTCAGAGG ACATAGACCT GCCCTACCCG
    CCACCTCAGA GGGAGGCCAA CATCTTCATG GTCCCCCAGA ACATCAAGCC TGTTCTCCAG
    CGTACTGCCA TAGAGATCCT GGCGTGGGGC CTGCGGAACA TGAAGAGCTA CCAGCTAGCC
    AGCATCTCCT CCCCTAGCCT TGTGGTAGAG TGCGGGGGCC AGACAGTGCA GTCCTGTGTC
    ATCAAGAACC TCCGGAAGAA CCCCAACTTC GACATCTGTA CCCTTTTCAT GGAAGTGATG
    CTGCCCAAGG AGGAGGTCTA CTGTCCCTCC ATCGTGATCA AGGTCATTGA TAACCGCCAG
    TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT ACCATCCGTT CCCTGGAGAG CTTCATGTGT
    GACCTGTACT CCGAGGAGAA TCCGTCCCCA CAGGGCGGCC CAGATGATGT GAGTTTACTC
    AGTCCTGGGG AAGACGTGCT CATTGACATC GATGACAAGG AGCCCCTCAT CCCCATGCAG
    GAGGAAGAGT TCATCGACTG GTGGAGCAAA TTCTTTGCCT CCACAGGGGA GAGGGAAAAG
    TGCGGCTCAT ACTTGGAGAA GGGCTTTGAC ACTCTAAAGG TCTATGACAC ACAACTGGAG
    AACGTGGAGG CCTTTGAGGG CCTGTCTGAC TTTTGTAACA CCTTCAAGCT CTACCGGGGC
    AAGACTCAGG AGGAGACAGA GGACCCATCT GTTATTGGGG AGTTTAAGGG CCTCTTCAAA
    ATCTATCCCC TCCCAGAAGA CCCGGCTGTT CCCATGCCCC CGAGACAATT CCACCAGCTG
    GCTGCCCAAG GGCCTCAGGA GTGCCTGGTC CGCATCTATA TCATCCGAGC ATTTGGCCTT
    CAGCCCAAGG ACCCCAATGG GAAGTGTGAT CCTTACATCA AGATCTCCAT AGGGAAGAAA
    TCAGTGAGTG ACCAGGATAA CTACATTCCC TGTACTCTGG AGCCTGTGTT TGGAAAGATG
    TTCGAGCTGA CCTGCACTCT GCCTCTGGAG AAGGACCTGA AGATCACACT CTATGACTAT
    GACCTCCTCT CCAAAGATGA AAAGATTGGG GAGACAGTCA TTGACCTGGA GGATAGGCTG
    CTGTCCAAGT TTGGGGCTCG CTGCGGACTC CCACAGACGT ACTGTGTCTC TGGACCCAAC
    CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG CTTCTCCACC TCTTCTGCCA GCAGCGCAGA
    CTCAAGGCTC CTGTGTACCT CACGGACCAC GTGGTGTTCC AGGATAAAGA ATACAGCATT
    GAAGAAATAG AGGCTGGCCG AGTCCCGAAC CCACACCTGG GCCCAGTGGA AGAGCGTCTG
    GCCTTGCATG TCCTCCAGCA GCAGGGCCTG GTCCCTGAGC ATGTGGAGTC CCGGCCTCTC
    TACAGCCCCC TGCAGCCAGA CATTGAACAG GGGAAGCTGC AGATGTGGAT CGACCTATTT
    CCCAAGGCCC TGGGGCGTCC CGGACCTCCC TTCAACATCA CCCCGCGGAG AGCCAGAAGG
    TTTTTCCTGC GTTGTATCAT CTGGAACACA AAGGACGTGA TCCTGGACGA CCTCAGCATC
    ACAGGGGAGA AGATGAGTGA TATTTATGTG AAGGGTTGGA TGGTTGGCTT TGAAGAGCAC
    AAGCAAAAGA CAGATGTGCA TTACCGGTCC CTGGGAGGTG AAGGCAACTT TAACTGGAGG
    TTCATTTTCC CCTTTGACTA CCTGCCAGCC GAGCAAGTCT GCACCGTCTC TAAGAAGGAT
    GCTTTCTGGA GGCTGGACAA GACTGAGAGC AAAATCCCAG CACGAGTGGT ATTTCAGATC
    TGGGACAATG ACAAGTTCTC CTTTGACGAC TTTCTGGGCT CCTTGCAGTT GGATCTCAAC
    CACATGCCCA AGCCTGCCAA GACAGCTGAG AAGTGCTCCT TGGACCAGCT GGATGACACT
    TTCCACCCGG AGTGGTTTGT GTCCCTTTTT GAACAGAAAA CAGTTAAGGG CTGGTGGCCC
    TGCGTAGCAG AAGAGGGCGA GAAGAAGATC CTGGCAGGCA AGCTGGAAAT GACCTTGGAG
    ATCGTAGCAG AGAGTGAGCA TGAGGAGCGG CCTGCTGGCC AGGGCCGGGA TGAACCCAAC
    ATGAACCCCA AGCTCGAGGA CCCAAGGCGC CCTGACACCT CCTTCCTCTG GTTCACCTCT
    CCATACAAGA CTATGAAGTT CATCCTGTGG CGGCGGTTCC GATGTGCCAT TATCCTCTTC
    ATCATTCTCT TCATCCTGCT ACTATTCTTA GGCATCTTCA TCTATGCTTT CCCGAACTAC
    GCTGCCATGA AGCTGGTGAA GCCCTTCAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe19219
    Clone ID Related Accession (Same CDS sequence) XM_003432223.3 , XM_003432223.4
    Accession Version XM_003432223.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6210bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product dysferlin isoform X14
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003432223.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC
    CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC
    TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA
    CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGACA CAGGAGGAGA GGAGGACACT
    GAGGACCAGG GGCTCACTGG AGACGAGGGA GAGCCATTAC TGGATCAGAG TGGCGTCCCA
    GGCCCTGGGG CTCCCACCAC CCCGAGGAAG CCACCTTCCC ATCTGCCCCC CTACCACCCT
    GGGAGCAAGA GGAAAAGAAG TGCTCCCGCA GCCAGAAAGC TGCTGTCCGA CAAACCGCAG
    GACTTCCAGA TCAGAGTGCA AGTGATCGAG GGCCGCCAGC TGCCAGGGGT GAATATCAAG
    CCTGTGATCA AGGTCACTGC TGCTGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC
    AGCCCACTAT TTAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTTC GGAGCTGTTT
    GATGAGCCCA TCTTTATCAC GGTCGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT
    GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACATGC TTATCTGAGG
    AAGTGGCTGC TACTCTCCAA CCCTGATGAC TTCTCTGTTG GGGCCAGAGG TTACCTGAAA
    GCAAGCCTTT ATGTGCTGGG GCCCGGAGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT
    GAAGACAAGG AGGACATTGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTGCGAGGA
    GCCCACTTCT GCCTGAAGAT CTTCAGAGCG GAGGACTTAC CACAAATGGA TGATGCCGTG
    ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAATTT GGTAGATCCC
    TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC
    AACCCTCAGT GGAACCAGAG CATCACTCTG CCTGCCATGT TTCCCTCCAT GTGTGAAAAA
    ATGAAGATTC GCGTCATGGA CTGGGACCGC CTTACCCACA ATGACATCGT GGCCACCACC
    TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGTGGA TGACCATCTG
    GGCTTCCTTC CCACCTTCGG GCCCTGCTTC GTCAATCTCT ATGGCAGCCC CAGGGAATTC
    ACTGGCTTCC CGGACCCCTA CGCGGAACTC AACATGGGCA AGGGGGAAGG CGTGGCCTAC
    CGAGGTCGGC TTCTGCTCTC CCTGGAGACC AAGCTGGTGG AGCACAGCGG CGAGCAAAGG
    GTGGAGGACC TCCCTGCTGA TGACATCCTT CGGGTGGAGA AATACCTCCG GAGGCGCAAG
    TACTCTCTCT TTGCCGCCTT CTACTCGGCC ACCATGCTCC AGGATGTCGA CGATGCCATC
    CAGTTTGAGG TCAGCATTGG GAACTATGGC AACAAGTTCG ACACGACCTG TCTGCCACTG
    GCCTCCACCA CCCAGTACAG CCGGGCAGTC TTTGATGGGT GCCACTACTA CTACTTGCCC
    TGGGGCAATG TGAAGCCTGT GGTGGTGCTG TCATCCTACT GGGAAGACAT CAGCCACAGA
    GTCGAGGCTC AGAACCAGCT GCTCAGGATC GCTGACCATC TGGAAGCTGG CCTGGAGCAA
    GTCCACTTGG CTTTAAAGGC GCAGTTCAAC ACAGAGGATG TGGACTCCCT GTTGGCCCAG
    CTGATGGATG AGCTCATCTC AGACTGCAGC CAGCCCCTGG GCAATGTCCA AGAGATACCC
    TCTGCCACCC ACCTGGACCG GCACCTGTAC AGGCTGCGCA CCCGTCACCT AGGCCAGATT
    ACTGAGGCGG CCCTGGCTCT GAAACTCGGG CATGGCAAGC TCCCTGCCGC CCTGGAGCAG
    GCTGAAGACT GGCTCCTGCG TCTCCGTGCC CTGGCTGATG AGCCCCAGAA CAGCCTGCCC
    GACATCATCA TCTGGATGCT GCAGGGAAAC AAGCGAGTGG CGTACCAGCG GGTGCCTGCG
    CACGAGGTCC TCTTCTCCCG GCGGGGCGCC AGCTACTGTG GCAAGAACTG TGGGAAGCTG
    CAGACGATAT TTCTGAAATA TCCCATGGAG GGGGTGCCCG GAGCCCGGAT GCCAGTACAA
    ATACGGGTGA AACTCTGGTT TGGGCTTTCT GTGGATGAGA AGGAGTTCAA CCAGTTTGCT
    GAGGGAAAGC TGTCCGTCTT TGCTGAAACT TATGAGAACC AGACCAAACT GGCCCTGGTT
    GGGAACTGGG GCACAACAGG CCTCACCTAC CCCAAGTTTT CTGATGTCAT GGGCAAGATC
    AAGCTACCCA AGGATAGCTT CCGCCCCTCA GCTGGCTGGA CCTGGGCCGG AGACTGGTTC
    GTGTGTCCAG AGAAGACCCT ACTCCATGAC ATGGACGCGG GCCACCTGAG TTTTGTGGAG
    GAGGTGTTTG AGAACCAGAC CCGGCTCCCT GGAGGCCAGT GGATCTACAT GAGTGACAAC
    TACACAGATG TGAATGGGGA TAAGGTGCTT CCTAAGGATG ACATTGAGTG CCCACTGGGC
    TGGAAGTGGG AAGATGAAGA GTGGTCCACG GACCTCAATC GGGCCGTCGA TGAGCAAGGC
    TGGGAGTACA GTATCACCAT CCCCCCGGAT CGGAAGCCAA AGCACTGGGT TCCTGCTGAG
    AAGATGTATT ACACGCACCG TCGGCGGCGC TGGGTCCGCC TGCGCAGAAG GGACCTCAGC
    CAGATGGAGG CATTGAAGAG GCACAGACAG GCAGAGGCAG AGGGTGAGGG CTGGGAGTAC
    GCCTCGCTCT TGGGCTGGAA GTTCCACCTG GAGTACCGCA AGACTGATGC TTTCCGCCGC
    CGCCGCTGGC GCCGCCGCAT GGAACCGCTG GAGAAGACGG GGCCTGCAGC CGTGTTTGCC
    CTTGAGGGGG CTCTGGGTGG TGTGGTAGAT GACAGGAGTG AAGATTCCGT GTCCGTGTCC
    ACCTTGAGCT TTGGAGTGAA CAGACCCACG ATTTCCTGCA TCTTTGATTA TGGGAACCGC
    TACCACCTAC GCTGCTACAT GTACCAGGCT CGGGACCTGC CCTCGATGGA CAAGGACTCT
    TTTTCTGATC CCTACGCCAT CGTCTCCTTC CTGCACCAGA GCCAGAAGAC AGTGGTGGCG
    AAGAACACTC TGAACCCCAC CTGGGACCAG ACGCTTATCT TCTATGAGAT TGAGATCTTT
    GGTGAGCCAG CCAGTATCGC TGAGCAGCCG CCCAGCATTG TGGTGGAGCT GTATGACCAC
    GACACCTACG GTGCAGATGA GTTTATGGGA CGCTGCATTT GTCAGCCCAG CCTGGAGCGG
    ACACCCCGGC TGGCCTGGTT CCCACTGATT AGGGGCAGCC AGTCGGCGGG GGAACTGCTG
    GCCTCTTTTG AGCTCATCCA GAGAGAAAAG CCGGCCATTC ACCATATTCC TGGTTTTGAG
    GTACAGGATA TGTCAGGAAT CCTGGAAGAG TCAGAGGACA TAGACCTGCC CTACCCGCCA
    CCTCAGAGGG AGGCCAACAT CTTCATGGTC CCCCAGAACA TCAAGCCTGT TCTCCAGCGT
    ACTGCCATAG AGATCCTGGC GTGGGGCCTG CGGAACATGA AGAGCTACCA GCTAGCCAGC
    ATCTCCTCCC CTAGCCTTGT GGTAGAGTGC GGGGGCCAGA CAGTGCAGTC CTGTGTCATC
    AAGAACCTCC GGAAGAACCC CAACTTCGAC ATCTGTACCC TTTTCATGGA AGTGATGCTG
    CCCAAGGAGG AGGTCTACTG TCCCTCCATC GTGATCAAGG TCATTGATAA CCGCCAGTTT
    GGCCGCCGGC CTGTGGTGGG CCAGTGTACC ATCCGTTCCC TGGAGAGCTT CATGTGTGAC
    CTGTACTCCG AGGAGAATCC GTCCCCACAG GGCGGCCCAG ATGATGTGAG TTTACTCAGT
    CCTGGGGAAG ACGTGCTCAT TGACATCGAT GACAAGGAGC CCCTCATCCC CATGCAGGAG
    GAAGAGTTCA TCGACTGGTG GAGCAAATTC TTTGCCTCCA CAGGGGAGAG GGAAAAGTGC
    GGCTCATACT TGGAGAAGGG CTTTGACACT CTAAAGGTCT ATGACACACA ACTGGAGAAC
    GTGGAGGCCT TTGAGGGCCT GTCTGACTTT TGTAACACCT TCAAGCTCTA CCGGGGCAAG
    ACTCAGGAGG AGACAGAGGA CCCATCTGTT ATTGGGGAGT TTAAGGGCCT CTTCAAAATC
    TATCCCCTCC CAGAAGACCC GGCTGTTCCC ATGCCCCCGA GACAATTCCA CCAGCTGGCT
    GCCCAAGGGC CTCAGGAGTG CCTGGTCCGC ATCTATATCA TCCGAGCATT TGGCCTTCAG
    CCCAAGGACC CCAATGGGAA GTGTGATCCT TACATCAAGA TCTCCATAGG GAAGAAATCA
    GTGAGTGACC AGGATAACTA CATTCCCTGT ACTCTGGAGC CTGTGTTTGG AAAGATGTTC
    GAGCTGACCT GCACTCTGCC TCTGGAGAAG GACCTGAAGA TCACACTCTA TGACTATGAC
    CTCCTCTCCA AAGATGAAAA GATTGGGGAG ACAGTCATTG ACCTGGAGGA TAGGCTGCTG
    TCCAAGTTTG GGGCTCGCTG CGGACTCCCA CAGACGTACT GTGTCTCTGG ACCCAACCAG
    TGGAGGGACC AGCTCCGCCC CTCCCAGCTT CTCCACCTCT TCTGCCAGCA GCGCAGACTC
    AAGGCTCCTG TGTACCTCAC GGACCACGTG GTGTTCCAGG ATAAAGAATA CAGCATTGAA
    GAAATAGAGG CTGGCCGAGT CCCGAACCCA CACCTGGGCC CAGTGGAAGA GCGTCTGGCC
    TTGCATGTCC TCCAGCAGCA GGGCCTGGTC CCTGAGCATG TGGAGTCCCG GCCTCTCTAC
    AGCCCCCTGC AGCCAGACAT TGAACAGGGG AAGCTGCAGA TGTGGATCGA CCTATTTCCC
    AAGGCCCTGG GGCGTCCCGG ACCTCCCTTC AACATCACCC CGCGGAGAGC CAGAAGGTTT
    TTCCTGCGTT GTATCATCTG GAACACAAAG GACGTGATCC TGGACGACCT CAGCATCACA
    GGGGAGAAGA TGAGTGATAT TTATGTGAAG GGTTGGATGG TTGGCTTTGA AGAGCACAAG
    CAAAAGACAG ATGTGCATTA CCGGTCCCTG GGAGGTGAAG GCAACTTTAA CTGGAGGTTC
    ATTTTCCCCT TTGACTACCT GCCAGCCGAG CAAGTCTGCA CCGTCTCTAA GAAGGATGCT
    TTCTGGAGGC TGGACAAGAC TGAGAGCAAA ATCCCAGCAC GAGTGGTATT TCAGATCTGG
    GACAATGACA AGTTCTCCTT TGACGACTTT CTGGGCTCCT TGCAGTTGGA TCTCAACCAC
    ATGCCCAAGC CTGCCAAGAC AGCTGAGAAG TGCTCCTTGG ACCAGCTGGA TGACACTTTC
    CACCCGGAGT GGTTTGTGTC CCTTTTTGAA CAGAAAACAG TTAAGGGCTG GTGGCCCTGC
    GTAGCAGAAG AGGGCGAGAA GAAGATCCTG GCAGGCAAGC TGGAAATGAC CTTGGAGATC
    GTAGCAGAGA GTGAGCATGA GGAGCGGCCT GCTGGCCAGG GCCGGGATGA ACCCAACATG
    AACCCCAAGC TCGAGGACCC AAGGCGCCCT GACACCTCCT TCCTCTGGTT CACCTCTCCA
    TACAAGACTA TGAAGTTCAT CCTGTGGCGG CGGTTCCGAT GTGCCATTAT CCTCTTCATC
    ATTCTCTTCA TCCTGCTACT ATTCTTAGGC ATCTTCATCT ATGCTTTCCC GAACTACGCT
    GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003432271.1
    CDS438..6647
    Translation

    Target ORF information:

    RefSeq Version XM_003432223.3
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris dysferlin (DYSF), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003432223.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC
    CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC
    TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA
    CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGACA CAGGAGGAGA GGAGGACACT
    GAGGACCAGG GGCTCACTGG AGACGAGGGA GAGCCATTAC TGGATCAGAG TGGCGTCCCA
    GGCCCTGGGG CTCCCACCAC CCCGAGGAAG CCACCTTCCC ATCTGCCCCC CTACCACCCT
    GGGAGCAAGA GGAAAAGAAG TGCTCCCGCA GCCAGAAAGC TGCTGTCCGA CAAACCGCAG
    GACTTCCAGA TCAGAGTGCA AGTGATCGAG GGCCGCCAGC TGCCAGGGGT GAATATCAAG
    CCTGTGATCA AGGTCACTGC TGCTGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC
    AGCCCACTAT TTAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTTC GGAGCTGTTT
    GATGAGCCCA TCTTTATCAC GGTCGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT
    GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACATGC TTATCTGAGG
    AAGTGGCTGC TACTCTCCAA CCCTGATGAC TTCTCTGTTG GGGCCAGAGG TTACCTGAAA
    GCAAGCCTTT ATGTGCTGGG GCCCGGAGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT
    GAAGACAAGG AGGACATTGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTGCGAGGA
    GCCCACTTCT GCCTGAAGAT CTTCAGAGCG GAGGACTTAC CACAAATGGA TGATGCCGTG
    ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAATTT GGTAGATCCC
    TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC
    AACCCTCAGT GGAACCAGAG CATCACTCTG CCTGCCATGT TTCCCTCCAT GTGTGAAAAA
    ATGAAGATTC GCGTCATGGA CTGGGACCGC CTTACCCACA ATGACATCGT GGCCACCACC
    TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGTGGA TGACCATCTG
    GGCTTCCTTC CCACCTTCGG GCCCTGCTTC GTCAATCTCT ATGGCAGCCC CAGGGAATTC
    ACTGGCTTCC CGGACCCCTA CGCGGAACTC AACATGGGCA AGGGGGAAGG CGTGGCCTAC
    CGAGGTCGGC TTCTGCTCTC CCTGGAGACC AAGCTGGTGG AGCACAGCGG CGAGCAAAGG
    GTGGAGGACC TCCCTGCTGA TGACATCCTT CGGGTGGAGA AATACCTCCG GAGGCGCAAG
    TACTCTCTCT TTGCCGCCTT CTACTCGGCC ACCATGCTCC AGGATGTCGA CGATGCCATC
    CAGTTTGAGG TCAGCATTGG GAACTATGGC AACAAGTTCG ACACGACCTG TCTGCCACTG
    GCCTCCACCA CCCAGTACAG CCGGGCAGTC TTTGATGGGT GCCACTACTA CTACTTGCCC
    TGGGGCAATG TGAAGCCTGT GGTGGTGCTG TCATCCTACT GGGAAGACAT CAGCCACAGA
    GTCGAGGCTC AGAACCAGCT GCTCAGGATC GCTGACCATC TGGAAGCTGG CCTGGAGCAA
    GTCCACTTGG CTTTAAAGGC GCAGTTCAAC ACAGAGGATG TGGACTCCCT GTTGGCCCAG
    CTGATGGATG AGCTCATCTC AGACTGCAGC CAGCCCCTGG GCAATGTCCA AGAGATACCC
    TCTGCCACCC ACCTGGACCG GCACCTGTAC AGGCTGCGCA CCCGTCACCT AGGCCAGATT
    ACTGAGGCGG CCCTGGCTCT GAAACTCGGG CATGGCAAGC TCCCTGCCGC CCTGGAGCAG
    GCTGAAGACT GGCTCCTGCG TCTCCGTGCC CTGGCTGATG AGCCCCAGAA CAGCCTGCCC
    GACATCATCA TCTGGATGCT GCAGGGAAAC AAGCGAGTGG CGTACCAGCG GGTGCCTGCG
    CACGAGGTCC TCTTCTCCCG GCGGGGCGCC AGCTACTGTG GCAAGAACTG TGGGAAGCTG
    CAGACGATAT TTCTGAAATA TCCCATGGAG GGGGTGCCCG GAGCCCGGAT GCCAGTACAA
    ATACGGGTGA AACTCTGGTT TGGGCTTTCT GTGGATGAGA AGGAGTTCAA CCAGTTTGCT
    GAGGGAAAGC TGTCCGTCTT TGCTGAAACT TATGAGAACC AGACCAAACT GGCCCTGGTT
    GGGAACTGGG GCACAACAGG CCTCACCTAC CCCAAGTTTT CTGATGTCAT GGGCAAGATC
    AAGCTACCCA AGGATAGCTT CCGCCCCTCA GCTGGCTGGA CCTGGGCCGG AGACTGGTTC
    GTGTGTCCAG AGAAGACCCT ACTCCATGAC ATGGACGCGG GCCACCTGAG TTTTGTGGAG
    GAGGTGTTTG AGAACCAGAC CCGGCTCCCT GGAGGCCAGT GGATCTACAT GAGTGACAAC
    TACACAGATG TGAATGGGGA TAAGGTGCTT CCTAAGGATG ACATTGAGTG CCCACTGGGC
    TGGAAGTGGG AAGATGAAGA GTGGTCCACG GACCTCAATC GGGCCGTCGA TGAGCAAGGC
    TGGGAGTACA GTATCACCAT CCCCCCGGAT CGGAAGCCAA AGCACTGGGT TCCTGCTGAG
    AAGATGTATT ACACGCACCG TCGGCGGCGC TGGGTCCGCC TGCGCAGAAG GGACCTCAGC
    CAGATGGAGG CATTGAAGAG GCACAGACAG GCAGAGGCAG AGGGTGAGGG CTGGGAGTAC
    GCCTCGCTCT TGGGCTGGAA GTTCCACCTG GAGTACCGCA AGACTGATGC TTTCCGCCGC
    CGCCGCTGGC GCCGCCGCAT GGAACCGCTG GAGAAGACGG GGCCTGCAGC CGTGTTTGCC
    CTTGAGGGGG CTCTGGGTGG TGTGGTAGAT GACAGGAGTG AAGATTCCGT GTCCGTGTCC
    ACCTTGAGCT TTGGAGTGAA CAGACCCACG ATTTCCTGCA TCTTTGATTA TGGGAACCGC
    TACCACCTAC GCTGCTACAT GTACCAGGCT CGGGACCTGC CCTCGATGGA CAAGGACTCT
    TTTTCTGATC CCTACGCCAT CGTCTCCTTC CTGCACCAGA GCCAGAAGAC AGTGGTGGCG
    AAGAACACTC TGAACCCCAC CTGGGACCAG ACGCTTATCT TCTATGAGAT TGAGATCTTT
    GGTGAGCCAG CCAGTATCGC TGAGCAGCCG CCCAGCATTG TGGTGGAGCT GTATGACCAC
    GACACCTACG GTGCAGATGA GTTTATGGGA CGCTGCATTT GTCAGCCCAG CCTGGAGCGG
    ACACCCCGGC TGGCCTGGTT CCCACTGATT AGGGGCAGCC AGTCGGCGGG GGAACTGCTG
    GCCTCTTTTG AGCTCATCCA GAGAGAAAAG CCGGCCATTC ACCATATTCC TGGTTTTGAG
    GTACAGGATA TGTCAGGAAT CCTGGAAGAG TCAGAGGACA TAGACCTGCC CTACCCGCCA
    CCTCAGAGGG AGGCCAACAT CTTCATGGTC CCCCAGAACA TCAAGCCTGT TCTCCAGCGT
    ACTGCCATAG AGATCCTGGC GTGGGGCCTG CGGAACATGA AGAGCTACCA GCTAGCCAGC
    ATCTCCTCCC CTAGCCTTGT GGTAGAGTGC GGGGGCCAGA CAGTGCAGTC CTGTGTCATC
    AAGAACCTCC GGAAGAACCC CAACTTCGAC ATCTGTACCC TTTTCATGGA AGTGATGCTG
    CCCAAGGAGG AGGTCTACTG TCCCTCCATC GTGATCAAGG TCATTGATAA CCGCCAGTTT
    GGCCGCCGGC CTGTGGTGGG CCAGTGTACC ATCCGTTCCC TGGAGAGCTT CATGTGTGAC
    CTGTACTCCG AGGAGAATCC GTCCCCACAG GGCGGCCCAG ATGATGTGAG TTTACTCAGT
    CCTGGGGAAG ACGTGCTCAT TGACATCGAT GACAAGGAGC CCCTCATCCC CATGCAGGAG
    GAAGAGTTCA TCGACTGGTG GAGCAAATTC TTTGCCTCCA CAGGGGAGAG GGAAAAGTGC
    GGCTCATACT TGGAGAAGGG CTTTGACACT CTAAAGGTCT ATGACACACA ACTGGAGAAC
    GTGGAGGCCT TTGAGGGCCT GTCTGACTTT TGTAACACCT TCAAGCTCTA CCGGGGCAAG
    ACTCAGGAGG AGACAGAGGA CCCATCTGTT ATTGGGGAGT TTAAGGGCCT CTTCAAAATC
    TATCCCCTCC CAGAAGACCC GGCTGTTCCC ATGCCCCCGA GACAATTCCA CCAGCTGGCT
    GCCCAAGGGC CTCAGGAGTG CCTGGTCCGC ATCTATATCA TCCGAGCATT TGGCCTTCAG
    CCCAAGGACC CCAATGGGAA GTGTGATCCT TACATCAAGA TCTCCATAGG GAAGAAATCA
    GTGAGTGACC AGGATAACTA CATTCCCTGT ACTCTGGAGC CTGTGTTTGG AAAGATGTTC
    GAGCTGACCT GCACTCTGCC TCTGGAGAAG GACCTGAAGA TCACACTCTA TGACTATGAC
    CTCCTCTCCA AAGATGAAAA GATTGGGGAG ACAGTCATTG ACCTGGAGGA TAGGCTGCTG
    TCCAAGTTTG GGGCTCGCTG CGGACTCCCA CAGACGTACT GTGTCTCTGG ACCCAACCAG
    TGGAGGGACC AGCTCCGCCC CTCCCAGCTT CTCCACCTCT TCTGCCAGCA GCGCAGACTC
    AAGGCTCCTG TGTACCTCAC GGACCACGTG GTGTTCCAGG ATAAAGAATA CAGCATTGAA
    GAAATAGAGG CTGGCCGAGT CCCGAACCCA CACCTGGGCC CAGTGGAAGA GCGTCTGGCC
    TTGCATGTCC TCCAGCAGCA GGGCCTGGTC CCTGAGCATG TGGAGTCCCG GCCTCTCTAC
    AGCCCCCTGC AGCCAGACAT TGAACAGGGG AAGCTGCAGA TGTGGATCGA CCTATTTCCC
    AAGGCCCTGG GGCGTCCCGG ACCTCCCTTC AACATCACCC CGCGGAGAGC CAGAAGGTTT
    TTCCTGCGTT GTATCATCTG GAACACAAAG GACGTGATCC TGGACGACCT CAGCATCACA
    GGGGAGAAGA TGAGTGATAT TTATGTGAAG GGTTGGATGG TTGGCTTTGA AGAGCACAAG
    CAAAAGACAG ATGTGCATTA CCGGTCCCTG GGAGGTGAAG GCAACTTTAA CTGGAGGTTC
    ATTTTCCCCT TTGACTACCT GCCAGCCGAG CAAGTCTGCA CCGTCTCTAA GAAGGATGCT
    TTCTGGAGGC TGGACAAGAC TGAGAGCAAA ATCCCAGCAC GAGTGGTATT TCAGATCTGG
    GACAATGACA AGTTCTCCTT TGACGACTTT CTGGGCTCCT TGCAGTTGGA TCTCAACCAC
    ATGCCCAAGC CTGCCAAGAC AGCTGAGAAG TGCTCCTTGG ACCAGCTGGA TGACACTTTC
    CACCCGGAGT GGTTTGTGTC CCTTTTTGAA CAGAAAACAG TTAAGGGCTG GTGGCCCTGC
    GTAGCAGAAG AGGGCGAGAA GAAGATCCTG GCAGGCAAGC TGGAAATGAC CTTGGAGATC
    GTAGCAGAGA GTGAGCATGA GGAGCGGCCT GCTGGCCAGG GCCGGGATGA ACCCAACATG
    AACCCCAAGC TCGAGGACCC AAGGCGCCCT GACACCTCCT TCCTCTGGTT CACCTCTCCA
    TACAAGACTA TGAAGTTCAT CCTGTGGCGG CGGTTCCGAT GTGCCATTAT CCTCTTCATC
    ATTCTCTTCA TCCTGCTACT ATTCTTAGGC ATCTTCATCT ATGCTTTCCC GAACTACGCT
    GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe19219
    Clone ID Related Accession (Same CDS sequence) XM_003432223.3 , XM_003432223.4
    Accession Version XM_003432223.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6210bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product dysferlin isoform X24
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003432223.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC
    CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC
    TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA
    CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGACA CAGGAGGAGA GGAGGACACT
    GAGGACCAGG GGCTCACTGG AGACGAGGGA GAGCCATTAC TGGATCAGAG TGGCGTCCCA
    GGCCCTGGGG CTCCCACCAC CCCGAGGAAG CCACCTTCCC ATCTGCCCCC CTACCACCCT
    GGGAGCAAGA GGAAAAGAAG TGCTCCCGCA GCCAGAAAGC TGCTGTCCGA CAAACCGCAG
    GACTTCCAGA TCAGAGTGCA AGTGATCGAG GGCCGCCAGC TGCCAGGGGT GAATATCAAG
    CCTGTGATCA AGGTCACTGC TGCTGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC
    AGCCCACTAT TTAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTTC GGAGCTGTTT
    GATGAGCCCA TCTTTATCAC GGTCGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT
    GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACATGC TTATCTGAGG
    AAGTGGCTGC TACTCTCCAA CCCTGATGAC TTCTCTGTTG GGGCCAGAGG TTACCTGAAA
    GCAAGCCTTT ATGTGCTGGG GCCCGGAGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT
    GAAGACAAGG AGGACATTGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTGCGAGGA
    GCCCACTTCT GCCTGAAGAT CTTCAGAGCG GAGGACTTAC CACAAATGGA TGATGCCGTG
    ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAATTT GGTAGATCCC
    TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC
    AACCCTCAGT GGAACCAGAG CATCACTCTG CCTGCCATGT TTCCCTCCAT GTGTGAAAAA
    ATGAAGATTC GCGTCATGGA CTGGGACCGC CTTACCCACA ATGACATCGT GGCCACCACC
    TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGTGGA TGACCATCTG
    GGCTTCCTTC CCACCTTCGG GCCCTGCTTC GTCAATCTCT ATGGCAGCCC CAGGGAATTC
    ACTGGCTTCC CGGACCCCTA CGCGGAACTC AACATGGGCA AGGGGGAAGG CGTGGCCTAC
    CGAGGTCGGC TTCTGCTCTC CCTGGAGACC AAGCTGGTGG AGCACAGCGG CGAGCAAAGG
    GTGGAGGACC TCCCTGCTGA TGACATCCTT CGGGTGGAGA AATACCTCCG GAGGCGCAAG
    TACTCTCTCT TTGCCGCCTT CTACTCGGCC ACCATGCTCC AGGATGTCGA CGATGCCATC
    CAGTTTGAGG TCAGCATTGG GAACTATGGC AACAAGTTCG ACACGACCTG TCTGCCACTG
    GCCTCCACCA CCCAGTACAG CCGGGCAGTC TTTGATGGGT GCCACTACTA CTACTTGCCC
    TGGGGCAATG TGAAGCCTGT GGTGGTGCTG TCATCCTACT GGGAAGACAT CAGCCACAGA
    GTCGAGGCTC AGAACCAGCT GCTCAGGATC GCTGACCATC TGGAAGCTGG CCTGGAGCAA
    GTCCACTTGG CTTTAAAGGC GCAGTTCAAC ACAGAGGATG TGGACTCCCT GTTGGCCCAG
    CTGATGGATG AGCTCATCTC AGACTGCAGC CAGCCCCTGG GCAATGTCCA AGAGATACCC
    TCTGCCACCC ACCTGGACCG GCACCTGTAC AGGCTGCGCA CCCGTCACCT AGGCCAGATT
    ACTGAGGCGG CCCTGGCTCT GAAACTCGGG CATGGCAAGC TCCCTGCCGC CCTGGAGCAG
    GCTGAAGACT GGCTCCTGCG TCTCCGTGCC CTGGCTGATG AGCCCCAGAA CAGCCTGCCC
    GACATCATCA TCTGGATGCT GCAGGGAAAC AAGCGAGTGG CGTACCAGCG GGTGCCTGCG
    CACGAGGTCC TCTTCTCCCG GCGGGGCGCC AGCTACTGTG GCAAGAACTG TGGGAAGCTG
    CAGACGATAT TTCTGAAATA TCCCATGGAG GGGGTGCCCG GAGCCCGGAT GCCAGTACAA
    ATACGGGTGA AACTCTGGTT TGGGCTTTCT GTGGATGAGA AGGAGTTCAA CCAGTTTGCT
    GAGGGAAAGC TGTCCGTCTT TGCTGAAACT TATGAGAACC AGACCAAACT GGCCCTGGTT
    GGGAACTGGG GCACAACAGG CCTCACCTAC CCCAAGTTTT CTGATGTCAT GGGCAAGATC
    AAGCTACCCA AGGATAGCTT CCGCCCCTCA GCTGGCTGGA CCTGGGCCGG AGACTGGTTC
    GTGTGTCCAG AGAAGACCCT ACTCCATGAC ATGGACGCGG GCCACCTGAG TTTTGTGGAG
    GAGGTGTTTG AGAACCAGAC CCGGCTCCCT GGAGGCCAGT GGATCTACAT GAGTGACAAC
    TACACAGATG TGAATGGGGA TAAGGTGCTT CCTAAGGATG ACATTGAGTG CCCACTGGGC
    TGGAAGTGGG AAGATGAAGA GTGGTCCACG GACCTCAATC GGGCCGTCGA TGAGCAAGGC
    TGGGAGTACA GTATCACCAT CCCCCCGGAT CGGAAGCCAA AGCACTGGGT TCCTGCTGAG
    AAGATGTATT ACACGCACCG TCGGCGGCGC TGGGTCCGCC TGCGCAGAAG GGACCTCAGC
    CAGATGGAGG CATTGAAGAG GCACAGACAG GCAGAGGCAG AGGGTGAGGG CTGGGAGTAC
    GCCTCGCTCT TGGGCTGGAA GTTCCACCTG GAGTACCGCA AGACTGATGC TTTCCGCCGC
    CGCCGCTGGC GCCGCCGCAT GGAACCGCTG GAGAAGACGG GGCCTGCAGC CGTGTTTGCC
    CTTGAGGGGG CTCTGGGTGG TGTGGTAGAT GACAGGAGTG AAGATTCCGT GTCCGTGTCC
    ACCTTGAGCT TTGGAGTGAA CAGACCCACG ATTTCCTGCA TCTTTGATTA TGGGAACCGC
    TACCACCTAC GCTGCTACAT GTACCAGGCT CGGGACCTGC CCTCGATGGA CAAGGACTCT
    TTTTCTGATC CCTACGCCAT CGTCTCCTTC CTGCACCAGA GCCAGAAGAC AGTGGTGGCG
    AAGAACACTC TGAACCCCAC CTGGGACCAG ACGCTTATCT TCTATGAGAT TGAGATCTTT
    GGTGAGCCAG CCAGTATCGC TGAGCAGCCG CCCAGCATTG TGGTGGAGCT GTATGACCAC
    GACACCTACG GTGCAGATGA GTTTATGGGA CGCTGCATTT GTCAGCCCAG CCTGGAGCGG
    ACACCCCGGC TGGCCTGGTT CCCACTGATT AGGGGCAGCC AGTCGGCGGG GGAACTGCTG
    GCCTCTTTTG AGCTCATCCA GAGAGAAAAG CCGGCCATTC ACCATATTCC TGGTTTTGAG
    GTACAGGATA TGTCAGGAAT CCTGGAAGAG TCAGAGGACA TAGACCTGCC CTACCCGCCA
    CCTCAGAGGG AGGCCAACAT CTTCATGGTC CCCCAGAACA TCAAGCCTGT TCTCCAGCGT
    ACTGCCATAG AGATCCTGGC GTGGGGCCTG CGGAACATGA AGAGCTACCA GCTAGCCAGC
    ATCTCCTCCC CTAGCCTTGT GGTAGAGTGC GGGGGCCAGA CAGTGCAGTC CTGTGTCATC
    AAGAACCTCC GGAAGAACCC CAACTTCGAC ATCTGTACCC TTTTCATGGA AGTGATGCTG
    CCCAAGGAGG AGGTCTACTG TCCCTCCATC GTGATCAAGG TCATTGATAA CCGCCAGTTT
    GGCCGCCGGC CTGTGGTGGG CCAGTGTACC ATCCGTTCCC TGGAGAGCTT CATGTGTGAC
    CTGTACTCCG AGGAGAATCC GTCCCCACAG GGCGGCCCAG ATGATGTGAG TTTACTCAGT
    CCTGGGGAAG ACGTGCTCAT TGACATCGAT GACAAGGAGC CCCTCATCCC CATGCAGGAG
    GAAGAGTTCA TCGACTGGTG GAGCAAATTC TTTGCCTCCA CAGGGGAGAG GGAAAAGTGC
    GGCTCATACT TGGAGAAGGG CTTTGACACT CTAAAGGTCT ATGACACACA ACTGGAGAAC
    GTGGAGGCCT TTGAGGGCCT GTCTGACTTT TGTAACACCT TCAAGCTCTA CCGGGGCAAG
    ACTCAGGAGG AGACAGAGGA CCCATCTGTT ATTGGGGAGT TTAAGGGCCT CTTCAAAATC
    TATCCCCTCC CAGAAGACCC GGCTGTTCCC ATGCCCCCGA GACAATTCCA CCAGCTGGCT
    GCCCAAGGGC CTCAGGAGTG CCTGGTCCGC ATCTATATCA TCCGAGCATT TGGCCTTCAG
    CCCAAGGACC CCAATGGGAA GTGTGATCCT TACATCAAGA TCTCCATAGG GAAGAAATCA
    GTGAGTGACC AGGATAACTA CATTCCCTGT ACTCTGGAGC CTGTGTTTGG AAAGATGTTC
    GAGCTGACCT GCACTCTGCC TCTGGAGAAG GACCTGAAGA TCACACTCTA TGACTATGAC
    CTCCTCTCCA AAGATGAAAA GATTGGGGAG ACAGTCATTG ACCTGGAGGA TAGGCTGCTG
    TCCAAGTTTG GGGCTCGCTG CGGACTCCCA CAGACGTACT GTGTCTCTGG ACCCAACCAG
    TGGAGGGACC AGCTCCGCCC CTCCCAGCTT CTCCACCTCT TCTGCCAGCA GCGCAGACTC
    AAGGCTCCTG TGTACCTCAC GGACCACGTG GTGTTCCAGG ATAAAGAATA CAGCATTGAA
    GAAATAGAGG CTGGCCGAGT CCCGAACCCA CACCTGGGCC CAGTGGAAGA GCGTCTGGCC
    TTGCATGTCC TCCAGCAGCA GGGCCTGGTC CCTGAGCATG TGGAGTCCCG GCCTCTCTAC
    AGCCCCCTGC AGCCAGACAT TGAACAGGGG AAGCTGCAGA TGTGGATCGA CCTATTTCCC
    AAGGCCCTGG GGCGTCCCGG ACCTCCCTTC AACATCACCC CGCGGAGAGC CAGAAGGTTT
    TTCCTGCGTT GTATCATCTG GAACACAAAG GACGTGATCC TGGACGACCT CAGCATCACA
    GGGGAGAAGA TGAGTGATAT TTATGTGAAG GGTTGGATGG TTGGCTTTGA AGAGCACAAG
    CAAAAGACAG ATGTGCATTA CCGGTCCCTG GGAGGTGAAG GCAACTTTAA CTGGAGGTTC
    ATTTTCCCCT TTGACTACCT GCCAGCCGAG CAAGTCTGCA CCGTCTCTAA GAAGGATGCT
    TTCTGGAGGC TGGACAAGAC TGAGAGCAAA ATCCCAGCAC GAGTGGTATT TCAGATCTGG
    GACAATGACA AGTTCTCCTT TGACGACTTT CTGGGCTCCT TGCAGTTGGA TCTCAACCAC
    ATGCCCAAGC CTGCCAAGAC AGCTGAGAAG TGCTCCTTGG ACCAGCTGGA TGACACTTTC
    CACCCGGAGT GGTTTGTGTC CCTTTTTGAA CAGAAAACAG TTAAGGGCTG GTGGCCCTGC
    GTAGCAGAAG AGGGCGAGAA GAAGATCCTG GCAGGCAAGC TGGAAATGAC CTTGGAGATC
    GTAGCAGAGA GTGAGCATGA GGAGCGGCCT GCTGGCCAGG GCCGGGATGA ACCCAACATG
    AACCCCAAGC TCGAGGACCC AAGGCGCCCT GACACCTCCT TCCTCTGGTT CACCTCTCCA
    TACAAGACTA TGAAGTTCAT CCTGTGGCGG CGGTTCCGAT GTGCCATTAT CCTCTTCATC
    ATTCTCTTCA TCCTGCTACT ATTCTTAGGC ATCTTCATCT ATGCTTTCCC GAACTACGCT
    GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003432271.1
    CDS405..6614
    Translation

    Target ORF information:

    RefSeq Version XM_003432223.4
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris dysferlin (DYSF), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003432223.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
    GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC
    AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC
    CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC
    TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC
    TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG
    GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA
    CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGACA CAGGAGGAGA GGAGGACACT
    GAGGACCAGG GGCTCACTGG AGACGAGGGA GAGCCATTAC TGGATCAGAG TGGCGTCCCA
    GGCCCTGGGG CTCCCACCAC CCCGAGGAAG CCACCTTCCC ATCTGCCCCC CTACCACCCT
    GGGAGCAAGA GGAAAAGAAG TGCTCCCGCA GCCAGAAAGC TGCTGTCCGA CAAACCGCAG
    GACTTCCAGA TCAGAGTGCA AGTGATCGAG GGCCGCCAGC TGCCAGGGGT GAATATCAAG
    CCTGTGATCA AGGTCACTGC TGCTGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC
    AGCCCACTAT TTAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTTC GGAGCTGTTT
    GATGAGCCCA TCTTTATCAC GGTCGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT
    GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACATGC TTATCTGAGG
    AAGTGGCTGC TACTCTCCAA CCCTGATGAC TTCTCTGTTG GGGCCAGAGG TTACCTGAAA
    GCAAGCCTTT ATGTGCTGGG GCCCGGAGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT
    GAAGACAAGG AGGACATTGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTGCGAGGA
    GCCCACTTCT GCCTGAAGAT CTTCAGAGCG GAGGACTTAC CACAAATGGA TGATGCCGTG
    ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAATTT GGTAGATCCC
    TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC
    AACCCTCAGT GGAACCAGAG CATCACTCTG CCTGCCATGT TTCCCTCCAT GTGTGAAAAA
    ATGAAGATTC GCGTCATGGA CTGGGACCGC CTTACCCACA ATGACATCGT GGCCACCACC
    TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGTGGA TGACCATCTG
    GGCTTCCTTC CCACCTTCGG GCCCTGCTTC GTCAATCTCT ATGGCAGCCC CAGGGAATTC
    ACTGGCTTCC CGGACCCCTA CGCGGAACTC AACATGGGCA AGGGGGAAGG CGTGGCCTAC
    CGAGGTCGGC TTCTGCTCTC CCTGGAGACC AAGCTGGTGG AGCACAGCGG CGAGCAAAGG
    GTGGAGGACC TCCCTGCTGA TGACATCCTT CGGGTGGAGA AATACCTCCG GAGGCGCAAG
    TACTCTCTCT TTGCCGCCTT CTACTCGGCC ACCATGCTCC AGGATGTCGA CGATGCCATC
    CAGTTTGAGG TCAGCATTGG GAACTATGGC AACAAGTTCG ACACGACCTG TCTGCCACTG
    GCCTCCACCA CCCAGTACAG CCGGGCAGTC TTTGATGGGT GCCACTACTA CTACTTGCCC
    TGGGGCAATG TGAAGCCTGT GGTGGTGCTG TCATCCTACT GGGAAGACAT CAGCCACAGA
    GTCGAGGCTC AGAACCAGCT GCTCAGGATC GCTGACCATC TGGAAGCTGG CCTGGAGCAA
    GTCCACTTGG CTTTAAAGGC GCAGTTCAAC ACAGAGGATG TGGACTCCCT GTTGGCCCAG
    CTGATGGATG AGCTCATCTC AGACTGCAGC CAGCCCCTGG GCAATGTCCA AGAGATACCC
    TCTGCCACCC ACCTGGACCG GCACCTGTAC AGGCTGCGCA CCCGTCACCT AGGCCAGATT
    ACTGAGGCGG CCCTGGCTCT GAAACTCGGG CATGGCAAGC TCCCTGCCGC CCTGGAGCAG
    GCTGAAGACT GGCTCCTGCG TCTCCGTGCC CTGGCTGATG AGCCCCAGAA CAGCCTGCCC
    GACATCATCA TCTGGATGCT GCAGGGAAAC AAGCGAGTGG CGTACCAGCG GGTGCCTGCG
    CACGAGGTCC TCTTCTCCCG GCGGGGCGCC AGCTACTGTG GCAAGAACTG TGGGAAGCTG
    CAGACGATAT TTCTGAAATA TCCCATGGAG GGGGTGCCCG GAGCCCGGAT GCCAGTACAA
    ATACGGGTGA AACTCTGGTT TGGGCTTTCT GTGGATGAGA AGGAGTTCAA CCAGTTTGCT
    GAGGGAAAGC TGTCCGTCTT TGCTGAAACT TATGAGAACC AGACCAAACT GGCCCTGGTT
    GGGAACTGGG GCACAACAGG CCTCACCTAC CCCAAGTTTT CTGATGTCAT GGGCAAGATC
    AAGCTACCCA AGGATAGCTT CCGCCCCTCA GCTGGCTGGA CCTGGGCCGG AGACTGGTTC
    GTGTGTCCAG AGAAGACCCT ACTCCATGAC ATGGACGCGG GCCACCTGAG TTTTGTGGAG
    GAGGTGTTTG AGAACCAGAC CCGGCTCCCT GGAGGCCAGT GGATCTACAT GAGTGACAAC
    TACACAGATG TGAATGGGGA TAAGGTGCTT CCTAAGGATG ACATTGAGTG CCCACTGGGC
    TGGAAGTGGG AAGATGAAGA GTGGTCCACG GACCTCAATC GGGCCGTCGA TGAGCAAGGC
    TGGGAGTACA GTATCACCAT CCCCCCGGAT CGGAAGCCAA AGCACTGGGT TCCTGCTGAG
    AAGATGTATT ACACGCACCG TCGGCGGCGC TGGGTCCGCC TGCGCAGAAG GGACCTCAGC
    CAGATGGAGG CATTGAAGAG GCACAGACAG GCAGAGGCAG AGGGTGAGGG CTGGGAGTAC
    GCCTCGCTCT TGGGCTGGAA GTTCCACCTG GAGTACCGCA AGACTGATGC TTTCCGCCGC
    CGCCGCTGGC GCCGCCGCAT GGAACCGCTG GAGAAGACGG GGCCTGCAGC CGTGTTTGCC
    CTTGAGGGGG CTCTGGGTGG TGTGGTAGAT GACAGGAGTG AAGATTCCGT GTCCGTGTCC
    ACCTTGAGCT TTGGAGTGAA CAGACCCACG ATTTCCTGCA TCTTTGATTA TGGGAACCGC
    TACCACCTAC GCTGCTACAT GTACCAGGCT CGGGACCTGC CCTCGATGGA CAAGGACTCT
    TTTTCTGATC CCTACGCCAT CGTCTCCTTC CTGCACCAGA GCCAGAAGAC AGTGGTGGCG
    AAGAACACTC TGAACCCCAC CTGGGACCAG ACGCTTATCT TCTATGAGAT TGAGATCTTT
    GGTGAGCCAG CCAGTATCGC TGAGCAGCCG CCCAGCATTG TGGTGGAGCT GTATGACCAC
    GACACCTACG GTGCAGATGA GTTTATGGGA CGCTGCATTT GTCAGCCCAG CCTGGAGCGG
    ACACCCCGGC TGGCCTGGTT CCCACTGATT AGGGGCAGCC AGTCGGCGGG GGAACTGCTG
    GCCTCTTTTG AGCTCATCCA GAGAGAAAAG CCGGCCATTC ACCATATTCC TGGTTTTGAG
    GTACAGGATA TGTCAGGAAT CCTGGAAGAG TCAGAGGACA TAGACCTGCC CTACCCGCCA
    CCTCAGAGGG AGGCCAACAT CTTCATGGTC CCCCAGAACA TCAAGCCTGT TCTCCAGCGT
    ACTGCCATAG AGATCCTGGC GTGGGGCCTG CGGAACATGA AGAGCTACCA GCTAGCCAGC
    ATCTCCTCCC CTAGCCTTGT GGTAGAGTGC GGGGGCCAGA CAGTGCAGTC CTGTGTCATC
    AAGAACCTCC GGAAGAACCC CAACTTCGAC ATCTGTACCC TTTTCATGGA AGTGATGCTG
    CCCAAGGAGG AGGTCTACTG TCCCTCCATC GTGATCAAGG TCATTGATAA CCGCCAGTTT
    GGCCGCCGGC CTGTGGTGGG CCAGTGTACC ATCCGTTCCC TGGAGAGCTT CATGTGTGAC
    CTGTACTCCG AGGAGAATCC GTCCCCACAG GGCGGCCCAG ATGATGTGAG TTTACTCAGT
    CCTGGGGAAG ACGTGCTCAT TGACATCGAT GACAAGGAGC CCCTCATCCC CATGCAGGAG
    GAAGAGTTCA TCGACTGGTG GAGCAAATTC TTTGCCTCCA CAGGGGAGAG GGAAAAGTGC
    GGCTCATACT TGGAGAAGGG CTTTGACACT CTAAAGGTCT ATGACACACA ACTGGAGAAC
    GTGGAGGCCT TTGAGGGCCT GTCTGACTTT TGTAACACCT TCAAGCTCTA CCGGGGCAAG
    ACTCAGGAGG AGACAGAGGA CCCATCTGTT ATTGGGGAGT TTAAGGGCCT CTTCAAAATC
    TATCCCCTCC CAGAAGACCC GGCTGTTCCC ATGCCCCCGA GACAATTCCA CCAGCTGGCT
    GCCCAAGGGC CTCAGGAGTG CCTGGTCCGC ATCTATATCA TCCGAGCATT TGGCCTTCAG
    CCCAAGGACC CCAATGGGAA GTGTGATCCT TACATCAAGA TCTCCATAGG GAAGAAATCA
    GTGAGTGACC AGGATAACTA CATTCCCTGT ACTCTGGAGC CTGTGTTTGG AAAGATGTTC
    GAGCTGACCT GCACTCTGCC TCTGGAGAAG GACCTGAAGA TCACACTCTA TGACTATGAC
    CTCCTCTCCA AAGATGAAAA GATTGGGGAG ACAGTCATTG ACCTGGAGGA TAGGCTGCTG
    TCCAAGTTTG GGGCTCGCTG CGGACTCCCA CAGACGTACT GTGTCTCTGG ACCCAACCAG
    TGGAGGGACC AGCTCCGCCC CTCCCAGCTT CTCCACCTCT TCTGCCAGCA GCGCAGACTC
    AAGGCTCCTG TGTACCTCAC GGACCACGTG GTGTTCCAGG ATAAAGAATA CAGCATTGAA
    GAAATAGAGG CTGGCCGAGT CCCGAACCCA CACCTGGGCC CAGTGGAAGA GCGTCTGGCC
    TTGCATGTCC TCCAGCAGCA GGGCCTGGTC CCTGAGCATG TGGAGTCCCG GCCTCTCTAC
    AGCCCCCTGC AGCCAGACAT TGAACAGGGG AAGCTGCAGA TGTGGATCGA CCTATTTCCC
    AAGGCCCTGG GGCGTCCCGG ACCTCCCTTC AACATCACCC CGCGGAGAGC CAGAAGGTTT
    TTCCTGCGTT GTATCATCTG GAACACAAAG GACGTGATCC TGGACGACCT CAGCATCACA
    GGGGAGAAGA TGAGTGATAT TTATGTGAAG GGTTGGATGG TTGGCTTTGA AGAGCACAAG
    CAAAAGACAG ATGTGCATTA CCGGTCCCTG GGAGGTGAAG GCAACTTTAA CTGGAGGTTC
    ATTTTCCCCT TTGACTACCT GCCAGCCGAG CAAGTCTGCA CCGTCTCTAA GAAGGATGCT
    TTCTGGAGGC TGGACAAGAC TGAGAGCAAA ATCCCAGCAC GAGTGGTATT TCAGATCTGG
    GACAATGACA AGTTCTCCTT TGACGACTTT CTGGGCTCCT TGCAGTTGGA TCTCAACCAC
    ATGCCCAAGC CTGCCAAGAC AGCTGAGAAG TGCTCCTTGG ACCAGCTGGA TGACACTTTC
    CACCCGGAGT GGTTTGTGTC CCTTTTTGAA CAGAAAACAG TTAAGGGCTG GTGGCCCTGC
    GTAGCAGAAG AGGGCGAGAA GAAGATCCTG GCAGGCAAGC TGGAAATGAC CTTGGAGATC
    GTAGCAGAGA GTGAGCATGA GGAGCGGCCT GCTGGCCAGG GCCGGGATGA ACCCAACATG
    AACCCCAAGC TCGAGGACCC AAGGCGCCCT GACACCTCCT TCCTCTGGTT CACCTCTCCA
    TACAAGACTA TGAAGTTCAT CCTGTGGCGG CGGTTCCGAT GTGCCATTAT CCTCTTCATC
    ATTCTCTTCA TCCTGCTACT ATTCTTAGGC ATCTTCATCT ATGCTTTCCC GAACTACGCT
    GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.