IBTK cDNA ORF clone, Canis lupus familiaris(dog)

The following IBTK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IBTK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe292612 XM_022426287.1
Latest version!
Canis lupus familiaris inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe39387 XM_022426286.1
Latest version!
Canis lupus familiaris inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe39387 XM_014118123.2
Latest version!
Canis lupus familiaris inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe15526 XM_005627571.3
Latest version!
Canis lupus familiaris inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe15523 XM_539018.6
Latest version!
Canis lupus familiaris inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe15524 XM_005627569.3
Latest version!
Canis lupus familiaris inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe15525 XM_005627570.3
Latest version!
Canis lupus familiaris inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OCe15526 XM_005627571.2 Canis lupus familiaris inhibitor of Bruton agammaglobulinemia tyrosine kinase (IBTK), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe15523 XM_539018.5 Canis lupus familiaris inhibitor of Bruton agammaglobulinemia tyrosine kinase (IBTK), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe15525 XM_005627570.2 Canis lupus familiaris inhibitor of Bruton agammaglobulinemia tyrosine kinase (IBTK), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe15524 XM_005627569.2 Canis lupus familiaris inhibitor of Bruton agammaglobulinemia tyrosine kinase (IBTK), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe39387 XM_014118123.1 Canis lupus familiaris inhibitor of Bruton agammaglobulinemia tyrosine kinase (IBTK), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe292612
    Clone ID Related Accession (Same CDS sequence) XM_022426287.1
    Accession Version XM_022426287.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3972bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product inhibitor of Bruton tyrosine kinase isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
    GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT
    TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC
    TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG
    AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT
    TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG
    TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT
    CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG
    AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG
    CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC
    TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT
    AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT
    ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA
    TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT
    CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT
    AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC
    AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC
    ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT
    ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA
    AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA
    AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG
    AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT
    TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT
    AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGCAGA GATTTTATCA
    AATCTTCATA ATTCCTCATC AGATGTGTCT TGTGTCTCTG ATATAAATAG TGTATACGAA
    AGAATTCGAC TTGAGAAACT TACTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGATCCA
    AGTGGATGCA ACTTTGCAAT CCTGCAGTCA GATCCTAAAA CAAGCCTTTA TGAAATTCCA
    GCCGTATCCT CATCATCCTT TTTTGAAGAG TTTGGCAAAC TCTTAAGGGA AACAGATGAA
    ACGGACAGTA TTCATGATGT GACATTTCAA GTTGGCAATA GAATTTTCCC TGCACATAAA
    TATATTTTGG CAGTGCGTTC TGACTTTTTT CAGAAATTGT TTCTTTCAGA TGGGACTACT
    TTAGATTTTA TAGATGTTTA CCGGAAAGAT GAAGATTCTG CAGGATGCCA TCTCTTTGTC
    ATAGAGAAGG TTCATCCTGA CTTGTTTGAG TACCTTTTAC AATTTATATA CACAGATACT
    TGTGACTTTT TAATTCATGG CTTCACACCA AGAATAAACT TAAACAAAAA GCCAGAAGAA
    TATCAGGGCA CTATGAATTC ACATTCAAAT AAAATGCATT GCCATGAAGA TAATCAGAAG
    TCAGCATTTG AAGTTTACAG AAGTAATCAA GCACACACAG TTAATGAAAA GCAAAAAAGC
    AAATCAAAGG CTTCCAATAA AGGGAAAAGT ATTGGGGAAG ATGATCCTGT AAGAATGTTG
    CAAAATGTTG CAAAGAAATT TGGTTTCAGT AATTTGAGTA GTAGTTCCTT TCTATGTGAT
    GTGACCATGA AATCAGTGGA TGGAAAGGAA TTTCCTTGTC ATAAGTGTGT TCTTTGTGCT
    AGACTTGAAT ATTTTCATAG TATGCTGAGT AGCTCATGGA TTGAGGCTTC TACTTGTACG
    GCTCTGGAAA TGCCAATACA TTCTGACATA CTGGAAGTTA TTTTGGACTA CCTCTATACT
    GATGAAGCTG TGGTGATAAA AGAATCTCAA AATGTGGATT TTGTTTGTAG TGTTCTCGTG
    GTGGCTGATC AGCTTCTTAT AACTCGATTG AAAGGGATTT GTGAAGTAGC ATTAACTGAG
    AAACTTACCC TTAAGAATGC TACTATGGTA CTGGAGTTTG CAGCAATGTA TAATGCTGAA
    CAATTGAAAC TGTCTTGTTT GCAGTTTATA GGACTGAATA TGGCCGCTTT ACTTGAAGCA
    AGGTCTCTTG ATGTTTTAAG TGATGGTGTT TTGAAGGATC TTTCTGTGTT TTATAGAAAA
    ATGATTCCAG CAATGGATAG AAGAGTCATT ACACCATACC AAGATGGACC AGATATTAGC
    TATTTGGAAG TAGAAGATGG AGATGTCTTT TTGAAAGAAG AAATAAATAT GGAACAAAAT
    TATTCGGAAA GTATGTTCAA GAAGGCAAAA ACAAAAGCAA AAAAGAAGCC GCGTAAGCGT
    TCAGATAGTT CTGGAGGTTA TAACCTTTCA GATGTTATTC AGAGTCCACC ATCTACAGGA
    TTATTAAAGT CTGGTAAGAA CAATTCTGTG GAATCTCTTC CAGAACTGTT GACATCAGAC
    TCTGAAGGAA GCTATGCAGG AGTGGGTAGT CCTAGAGATT TACAGTCCCC TGATTTCACA
    GCAGGATTTC AGTCAGATAA AATTGAGGTG AAAATTAAAC CATATGTTAA TGGTACACCT
    CCTGCATATT CTAGGGAAGA TTTAAAACCA TGGGAAAAAT CACCAATACT TAACATATCT
    GCTCCACAGA CCATTCCTAG TAACAGAATT GATACTACAA ACTCTTCTAG TTGGGTTGCT
    GGCTCTTTCA GTCCTGTCAG CCCTCCTGTT ATGGATCTCA GAACCATCAT GCAAATTGAA
    GAAAGTAGAC AAAAATGTGG AGCTACACCA AAGACAAATT TGGGCAAGAT GATTTCTCAT
    GGAGTTAAAC TTTCTCAGAA GCAACGGAAA ATGATTGCAA TGACTACCAA AGAAAACAAT
    TCAGGAACGA ATAGCATGGA AACAGCTTTA ACTGCTCCTT CAAAAACTCC AAAACCAGCA
    AACGCATGGG CATCTTCTGT ACATTCAGTT TCATCCAAGT CATTCCGGGA TTTCTTTGAA
    GAAAAAAAAT CTATTACTGG CCATAGTTCA GGAGATCATG TCAAAAAAGT TTGTTTTAAA
    GGAATAGAAA ATTCTCAGGC ATCAAAAGTT GAAAGATGTT CCCCTCATGG TACGCCAGGA
    CCAGAAGGCA ACCATATTTC AGATTTACCA CTTCTAGACA GTCCCAATCC CTGGCTGTCT
    TCTTCATTGA CTGCTCCTCC TGTGGTAGCT CCAGTCACTT TTGCATCTAT TGTAGAAGAA
    GAATTACAGC AGGAAGCAGC TTTAATCAGA AGTCGAGAAA AACCCTTGGC TTTGATTCAG
    ATTGAAGAGC GTGCAATACA AGATTTATTG GTTTTCTATG AGGCATTTGG CAACCCTGAT
    GAGTTTGTTA TTGTTGAAAG GACACCACAG GGACCACTAG CAGTACCTAT GTGGAATAAG
    CATGGATGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022281995.1
    CDS556..4527
    Translation

    Target ORF information:

    RefSeq Version XM_022426287.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426287.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
    GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT
    TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC
    TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG
    AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT
    TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG
    TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT
    CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG
    AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG
    CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC
    TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT
    AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT
    ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA
    TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT
    CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT
    AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC
    AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC
    ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT
    ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA
    AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA
    AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG
    AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT
    TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT
    AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGCAGA GATTTTATCA
    AATCTTCATA ATTCCTCATC AGATGTGTCT TGTGTCTCTG ATATAAATAG TGTATACGAA
    AGAATTCGAC TTGAGAAACT TACTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGATCCA
    AGTGGATGCA ACTTTGCAAT CCTGCAGTCA GATCCTAAAA CAAGCCTTTA TGAAATTCCA
    GCCGTATCCT CATCATCCTT TTTTGAAGAG TTTGGCAAAC TCTTAAGGGA AACAGATGAA
    ACGGACAGTA TTCATGATGT GACATTTCAA GTTGGCAATA GAATTTTCCC TGCACATAAA
    TATATTTTGG CAGTGCGTTC TGACTTTTTT CAGAAATTGT TTCTTTCAGA TGGGACTACT
    TTAGATTTTA TAGATGTTTA CCGGAAAGAT GAAGATTCTG CAGGATGCCA TCTCTTTGTC
    ATAGAGAAGG TTCATCCTGA CTTGTTTGAG TACCTTTTAC AATTTATATA CACAGATACT
    TGTGACTTTT TAATTCATGG CTTCACACCA AGAATAAACT TAAACAAAAA GCCAGAAGAA
    TATCAGGGCA CTATGAATTC ACATTCAAAT AAAATGCATT GCCATGAAGA TAATCAGAAG
    TCAGCATTTG AAGTTTACAG AAGTAATCAA GCACACACAG TTAATGAAAA GCAAAAAAGC
    AAATCAAAGG CTTCCAATAA AGGGAAAAGT ATTGGGGAAG ATGATCCTGT AAGAATGTTG
    CAAAATGTTG CAAAGAAATT TGGTTTCAGT AATTTGAGTA GTAGTTCCTT TCTATGTGAT
    GTGACCATGA AATCAGTGGA TGGAAAGGAA TTTCCTTGTC ATAAGTGTGT TCTTTGTGCT
    AGACTTGAAT ATTTTCATAG TATGCTGAGT AGCTCATGGA TTGAGGCTTC TACTTGTACG
    GCTCTGGAAA TGCCAATACA TTCTGACATA CTGGAAGTTA TTTTGGACTA CCTCTATACT
    GATGAAGCTG TGGTGATAAA AGAATCTCAA AATGTGGATT TTGTTTGTAG TGTTCTCGTG
    GTGGCTGATC AGCTTCTTAT AACTCGATTG AAAGGGATTT GTGAAGTAGC ATTAACTGAG
    AAACTTACCC TTAAGAATGC TACTATGGTA CTGGAGTTTG CAGCAATGTA TAATGCTGAA
    CAATTGAAAC TGTCTTGTTT GCAGTTTATA GGACTGAATA TGGCCGCTTT ACTTGAAGCA
    AGGTCTCTTG ATGTTTTAAG TGATGGTGTT TTGAAGGATC TTTCTGTGTT TTATAGAAAA
    ATGATTCCAG CAATGGATAG AAGAGTCATT ACACCATACC AAGATGGACC AGATATTAGC
    TATTTGGAAG TAGAAGATGG AGATGTCTTT TTGAAAGAAG AAATAAATAT GGAACAAAAT
    TATTCGGAAA GTATGTTCAA GAAGGCAAAA ACAAAAGCAA AAAAGAAGCC GCGTAAGCGT
    TCAGATAGTT CTGGAGGTTA TAACCTTTCA GATGTTATTC AGAGTCCACC ATCTACAGGA
    TTATTAAAGT CTGGTAAGAA CAATTCTGTG GAATCTCTTC CAGAACTGTT GACATCAGAC
    TCTGAAGGAA GCTATGCAGG AGTGGGTAGT CCTAGAGATT TACAGTCCCC TGATTTCACA
    GCAGGATTTC AGTCAGATAA AATTGAGGTG AAAATTAAAC CATATGTTAA TGGTACACCT
    CCTGCATATT CTAGGGAAGA TTTAAAACCA TGGGAAAAAT CACCAATACT TAACATATCT
    GCTCCACAGA CCATTCCTAG TAACAGAATT GATACTACAA ACTCTTCTAG TTGGGTTGCT
    GGCTCTTTCA GTCCTGTCAG CCCTCCTGTT ATGGATCTCA GAACCATCAT GCAAATTGAA
    GAAAGTAGAC AAAAATGTGG AGCTACACCA AAGACAAATT TGGGCAAGAT GATTTCTCAT
    GGAGTTAAAC TTTCTCAGAA GCAACGGAAA ATGATTGCAA TGACTACCAA AGAAAACAAT
    TCAGGAACGA ATAGCATGGA AACAGCTTTA ACTGCTCCTT CAAAAACTCC AAAACCAGCA
    AACGCATGGG CATCTTCTGT ACATTCAGTT TCATCCAAGT CATTCCGGGA TTTCTTTGAA
    GAAAAAAAAT CTATTACTGG CCATAGTTCA GGAGATCATG TCAAAAAAGT TTGTTTTAAA
    GGAATAGAAA ATTCTCAGGC ATCAAAAGTT GAAAGATGTT CCCCTCATGG TACGCCAGGA
    CCAGAAGGCA ACCATATTTC AGATTTACCA CTTCTAGACA GTCCCAATCC CTGGCTGTCT
    TCTTCATTGA CTGCTCCTCC TGTGGTAGCT CCAGTCACTT TTGCATCTAT TGTAGAAGAA
    GAATTACAGC AGGAAGCAGC TTTAATCAGA AGTCGAGAAA AACCCTTGGC TTTGATTCAG
    ATTGAAGAGC GTGCAATACA AGATTTATTG GTTTTCTATG AGGCATTTGG CAACCCTGAT
    GAGTTTGTTA TTGTTGAAAG GACACCACAG GGACCACTAG CAGTACCTAT GTGGAATAAG
    CATGGATGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39387
    Clone ID Related Accession (Same CDS sequence) XM_022426286.1 , XM_014118123.1 , XM_014118123.2
    Accession Version XM_022426286.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4059bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product inhibitor of Bruton tyrosine kinase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
    GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT
    TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC
    TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG
    AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT
    TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG
    TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT
    CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG
    AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG
    CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC
    TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT
    AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT
    ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA
    TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT
    CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT
    AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC
    AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC
    ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT
    ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA
    AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA
    AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG
    AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT
    TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT
    AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGCAGA GATTTTATCA
    AATCTTCATA ATTCCTCATC AGATGTGTCT TGTGTCTCTG ATATAAATAG TGTATACGAA
    AGAATTCGAC TTGAGAAACT TACTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGATCCA
    AGTGGATGCA ACTTTGCAAT CCTGCAGTCA GATCCTAAAA CAAGCCTTTA TGAAATTCCA
    GCCGTATCCT CATCATCCTT TTTTGAAGAG TTTGGCAAAC TCTTAAGGGA AACAGATGAA
    ACGGACAGTA TTCATGATGT GACATTTCAA GTTGGCAATA GAATTTTCCC TGCACATAAA
    TATATTTTGG CAGTGCGTTC TGACTTTTTT CAGAAATTGT TTCTTTCAGA TGGGACTACT
    TTAGATTTTA TAGATGTTTA CCGGAAAGAT GAAGATTCTG CAGGATGCCA TCTCTTTGTC
    ATAGAGAAGG TTCATCCTGA CTTGTTTGAG TACCTTTTAC AATTTATATA CACAGATACT
    TGTGACTTTT TAATTCATGG CTTCACACCA AGAATAAACT TAAACAAAAA GCCAGAAGAA
    TATCAGGGCA CTATGAATTC ACATTCAAAT AAAATGCATT GCCATGAAGA TAATCAGAAG
    TCAGCATTTG AAGTTTACAG AAGTAATCAA GCACACACAG TTAATGAAAA GCAAAAAAGC
    AAATCAAAGG CTTCCAATAA AGGGAAAAGT ATTGGGGAAG ATGATCCTGT AAGAATGTTG
    CAAAATGTTG CAAAGAAATT TGGTTTCAGT AATTTGAGTA GTAGGTTAGA TGGAGTCAGA
    TTTGAAAATG GAAAAATTAA TGTTATTGAG AAGAAAACTG GTAATAAATC AAAGCTAAAT
    CAGAAAAAAT GTTCCTTTCT ATGTGATGTG ACCATGAAAT CAGTGGATGG AAAGGAATTT
    CCTTGTCATA AGTGTGTTCT TTGTGCTAGA CTTGAATATT TTCATAGTAT GCTGAGTAGC
    TCATGGATTG AGGCTTCTAC TTGTACGGCT CTGGAAATGC CAATACATTC TGACATACTG
    GAAGTTATTT TGGACTACCT CTATACTGAT GAAGCTGTGG TGATAAAAGA ATCTCAAAAT
    GTGGATTTTG TTTGTAGTGT TCTCGTGGTG GCTGATCAGC TTCTTATAAC TCGATTGAAA
    GGGATTTGTG AAGTAGCATT AACTGAGAAA CTTACCCTTA AGAATGCTAC TATGGTACTG
    GAGTTTGCAG CAATGTATAA TGCTGAACAA TTGAAACTGT CTTGTTTGCA GTTTATAGGA
    CTGAATATGG CCGCTTTACT TGAAGCAAGG TCTCTTGATG TTTTAAGTGA TGGTGTTTTG
    AAGGATCTTT CTGTGTTTTA TAGAAAAATG ATTCCAGCAA TGGATAGAAG AGTCATTACA
    CCATACCAAG ATGGACCAGA TATTAGCTAT TTGGAAGTAG AAGATGGAGA TGTCTTTTTG
    AAAGAAGAAA TAAATATGGA ACAAAATTAT TCGGAAAGTA TGTTCAAGAA GGCAAAAACA
    AAAGCAAAAA AGAAGCCGCG TAAGCGTTCA GATAGTTCTG GAGGTTATAA CCTTTCAGAT
    GTTATTCAGA GTCCACCATC TACAGGATTA TTAAAGTCTG GTAAGAACAA TTCTGTGGAA
    TCTCTTCCAG AACTGTTGAC ATCAGACTCT GAAGGAAGCT ATGCAGGAGT GGGTAGTCCT
    AGAGATTTAC AGTCCCCTGA TTTCACAGCA GGATTTCAGT CAGATAAAAT TGAGGTGAAA
    ATTAAACCAT ATGTTAATGG TACACCTCCT GCATATTCTA GGGAAGATTT AAAACCATGG
    GAAAAATCAC CAATACTTAA CATATCTGCT CCACAGACCA TTCCTAGTAA CAGAATTGAT
    ACTACAAACT CTTCTAGTTG GGTTGCTGGC TCTTTCAGTC CTGTCAGCCC TCCTGTTATG
    GATCTCAGAA CCATCATGCA AATTGAAGAA AGTAGACAAA AATGTGGAGC TACACCAAAG
    ACAAATTTGG GCAAGATGAT TTCTCATGGA GTTAAACTTT CTCAGAAGCA ACGGAAAATG
    ATTGCAATGA CTACCAAAGA AAACAATTCA GGAACGAATA GCATGGAAAC AGCTTTAACT
    GCTCCTTCAA AAACTCCAAA ACCAGCAAAC GCATGGGCAT CTTCTGTACA TTCAGTTTCA
    TCCAAGTCAT TCCGGGATTT CTTTGAAGAA AAAAAATCTA TTACTGGCCA TAGTTCAGGA
    GATCATGTCA AAAAAGTTTG TTTTAAAGGA ATAGAAAATT CTCAGGCATC AAAAGTTGAA
    AGATGTTCCC CTCATGGTAC GCCAGGACCA GAAGGCAACC ATATTTCAGA TTTACCACTT
    CTAGACAGTC CCAATCCCTG GCTGTCTTCT TCATTGACTG CTCCTCCTGT GGTAGCTCCA
    GTCACTTTTG CATCTATTGT AGAAGAAGAA TTACAGCAGG AAGCAGCTTT AATCAGAAGT
    CGAGAAAAAC CCTTGGCTTT GATTCAGATT GAAGAGCGTG CAATACAAGA TTTATTGGTT
    TTCTATGAGG CATTTGGCAA CCCTGATGAG TTTGTTATTG TTGAAAGGAC ACCACAGGGA
    CCACTAGCAG TACCTATGTG GAATAAGCAT GGATGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022281994.1
    CDS608..4666
    Translation

    Target ORF information:

    RefSeq Version XM_022426286.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426286.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
    GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT
    TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC
    TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG
    AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT
    TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG
    TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT
    CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG
    AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG
    CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC
    TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT
    AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT
    ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA
    TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT
    CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT
    AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC
    AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC
    ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT
    ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA
    AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA
    AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG
    AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT
    TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT
    AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGCAGA GATTTTATCA
    AATCTTCATA ATTCCTCATC AGATGTGTCT TGTGTCTCTG ATATAAATAG TGTATACGAA
    AGAATTCGAC TTGAGAAACT TACTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGATCCA
    AGTGGATGCA ACTTTGCAAT CCTGCAGTCA GATCCTAAAA CAAGCCTTTA TGAAATTCCA
    GCCGTATCCT CATCATCCTT TTTTGAAGAG TTTGGCAAAC TCTTAAGGGA AACAGATGAA
    ACGGACAGTA TTCATGATGT GACATTTCAA GTTGGCAATA GAATTTTCCC TGCACATAAA
    TATATTTTGG CAGTGCGTTC TGACTTTTTT CAGAAATTGT TTCTTTCAGA TGGGACTACT
    TTAGATTTTA TAGATGTTTA CCGGAAAGAT GAAGATTCTG CAGGATGCCA TCTCTTTGTC
    ATAGAGAAGG TTCATCCTGA CTTGTTTGAG TACCTTTTAC AATTTATATA CACAGATACT
    TGTGACTTTT TAATTCATGG CTTCACACCA AGAATAAACT TAAACAAAAA GCCAGAAGAA
    TATCAGGGCA CTATGAATTC ACATTCAAAT AAAATGCATT GCCATGAAGA TAATCAGAAG
    TCAGCATTTG AAGTTTACAG AAGTAATCAA GCACACACAG TTAATGAAAA GCAAAAAAGC
    AAATCAAAGG CTTCCAATAA AGGGAAAAGT ATTGGGGAAG ATGATCCTGT AAGAATGTTG
    CAAAATGTTG CAAAGAAATT TGGTTTCAGT AATTTGAGTA GTAGGTTAGA TGGAGTCAGA
    TTTGAAAATG GAAAAATTAA TGTTATTGAG AAGAAAACTG GTAATAAATC AAAGCTAAAT
    CAGAAAAAAT GTTCCTTTCT ATGTGATGTG ACCATGAAAT CAGTGGATGG AAAGGAATTT
    CCTTGTCATA AGTGTGTTCT TTGTGCTAGA CTTGAATATT TTCATAGTAT GCTGAGTAGC
    TCATGGATTG AGGCTTCTAC TTGTACGGCT CTGGAAATGC CAATACATTC TGACATACTG
    GAAGTTATTT TGGACTACCT CTATACTGAT GAAGCTGTGG TGATAAAAGA ATCTCAAAAT
    GTGGATTTTG TTTGTAGTGT TCTCGTGGTG GCTGATCAGC TTCTTATAAC TCGATTGAAA
    GGGATTTGTG AAGTAGCATT AACTGAGAAA CTTACCCTTA AGAATGCTAC TATGGTACTG
    GAGTTTGCAG CAATGTATAA TGCTGAACAA TTGAAACTGT CTTGTTTGCA GTTTATAGGA
    CTGAATATGG CCGCTTTACT TGAAGCAAGG TCTCTTGATG TTTTAAGTGA TGGTGTTTTG
    AAGGATCTTT CTGTGTTTTA TAGAAAAATG ATTCCAGCAA TGGATAGAAG AGTCATTACA
    CCATACCAAG ATGGACCAGA TATTAGCTAT TTGGAAGTAG AAGATGGAGA TGTCTTTTTG
    AAAGAAGAAA TAAATATGGA ACAAAATTAT TCGGAAAGTA TGTTCAAGAA GGCAAAAACA
    AAAGCAAAAA AGAAGCCGCG TAAGCGTTCA GATAGTTCTG GAGGTTATAA CCTTTCAGAT
    GTTATTCAGA GTCCACCATC TACAGGATTA TTAAAGTCTG GTAAGAACAA TTCTGTGGAA
    TCTCTTCCAG AACTGTTGAC ATCAGACTCT GAAGGAAGCT ATGCAGGAGT GGGTAGTCCT
    AGAGATTTAC AGTCCCCTGA TTTCACAGCA GGATTTCAGT CAGATAAAAT TGAGGTGAAA
    ATTAAACCAT ATGTTAATGG TACACCTCCT GCATATTCTA GGGAAGATTT AAAACCATGG
    GAAAAATCAC CAATACTTAA CATATCTGCT CCACAGACCA TTCCTAGTAA CAGAATTGAT
    ACTACAAACT CTTCTAGTTG GGTTGCTGGC TCTTTCAGTC CTGTCAGCCC TCCTGTTATG
    GATCTCAGAA CCATCATGCA AATTGAAGAA AGTAGACAAA AATGTGGAGC TACACCAAAG
    ACAAATTTGG GCAAGATGAT TTCTCATGGA GTTAAACTTT CTCAGAAGCA ACGGAAAATG
    ATTGCAATGA CTACCAAAGA AAACAATTCA GGAACGAATA GCATGGAAAC AGCTTTAACT
    GCTCCTTCAA AAACTCCAAA ACCAGCAAAC GCATGGGCAT CTTCTGTACA TTCAGTTTCA
    TCCAAGTCAT TCCGGGATTT CTTTGAAGAA AAAAAATCTA TTACTGGCCA TAGTTCAGGA
    GATCATGTCA AAAAAGTTTG TTTTAAAGGA ATAGAAAATT CTCAGGCATC AAAAGTTGAA
    AGATGTTCCC CTCATGGTAC GCCAGGACCA GAAGGCAACC ATATTTCAGA TTTACCACTT
    CTAGACAGTC CCAATCCCTG GCTGTCTTCT TCATTGACTG CTCCTCCTGT GGTAGCTCCA
    GTCACTTTTG CATCTATTGT AGAAGAAGAA TTACAGCAGG AAGCAGCTTT AATCAGAAGT
    CGAGAAAAAC CCTTGGCTTT GATTCAGATT GAAGAGCGTG CAATACAAGA TTTATTGGTT
    TTCTATGAGG CATTTGGCAA CCCTGATGAG TTTGTTATTG TTGAAAGGAC ACCACAGGGA
    CCACTAGCAG TACCTATGTG GAATAAGCAT GGATGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39387
    Clone ID Related Accession (Same CDS sequence) XM_022426286.1 , XM_014118123.1 , XM_014118123.2
    Accession Version XM_014118123.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4059bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product inhibitor of Bruton tyrosine kinase isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
    GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT
    TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC
    TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG
    AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT
    TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG
    TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT
    CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG
    AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG
    CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC
    TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT
    AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT
    ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA
    TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT
    CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT
    AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC
    AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC
    ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT
    ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA
    AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA
    AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG
    AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT
    TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT
    AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGCAGA GATTTTATCA
    AATCTTCATA ATTCCTCATC AGATGTGTCT TGTGTCTCTG ATATAAATAG TGTATACGAA
    AGAATTCGAC TTGAGAAACT TACTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGATCCA
    AGTGGATGCA ACTTTGCAAT CCTGCAGTCA GATCCTAAAA CAAGCCTTTA TGAAATTCCA
    GCCGTATCCT CATCATCCTT TTTTGAAGAG TTTGGCAAAC TCTTAAGGGA AACAGATGAA
    ACGGACAGTA TTCATGATGT GACATTTCAA GTTGGCAATA GAATTTTCCC TGCACATAAA
    TATATTTTGG CAGTGCGTTC TGACTTTTTT CAGAAATTGT TTCTTTCAGA TGGGACTACT
    TTAGATTTTA TAGATGTTTA CCGGAAAGAT GAAGATTCTG CAGGATGCCA TCTCTTTGTC
    ATAGAGAAGG TTCATCCTGA CTTGTTTGAG TACCTTTTAC AATTTATATA CACAGATACT
    TGTGACTTTT TAATTCATGG CTTCACACCA AGAATAAACT TAAACAAAAA GCCAGAAGAA
    TATCAGGGCA CTATGAATTC ACATTCAAAT AAAATGCATT GCCATGAAGA TAATCAGAAG
    TCAGCATTTG AAGTTTACAG AAGTAATCAA GCACACACAG TTAATGAAAA GCAAAAAAGC
    AAATCAAAGG CTTCCAATAA AGGGAAAAGT ATTGGGGAAG ATGATCCTGT AAGAATGTTG
    CAAAATGTTG CAAAGAAATT TGGTTTCAGT AATTTGAGTA GTAGGTTAGA TGGAGTCAGA
    TTTGAAAATG GAAAAATTAA TGTTATTGAG AAGAAAACTG GTAATAAATC AAAGCTAAAT
    CAGAAAAAAT GTTCCTTTCT ATGTGATGTG ACCATGAAAT CAGTGGATGG AAAGGAATTT
    CCTTGTCATA AGTGTGTTCT TTGTGCTAGA CTTGAATATT TTCATAGTAT GCTGAGTAGC
    TCATGGATTG AGGCTTCTAC TTGTACGGCT CTGGAAATGC CAATACATTC TGACATACTG
    GAAGTTATTT TGGACTACCT CTATACTGAT GAAGCTGTGG TGATAAAAGA ATCTCAAAAT
    GTGGATTTTG TTTGTAGTGT TCTCGTGGTG GCTGATCAGC TTCTTATAAC TCGATTGAAA
    GGGATTTGTG AAGTAGCATT AACTGAGAAA CTTACCCTTA AGAATGCTAC TATGGTACTG
    GAGTTTGCAG CAATGTATAA TGCTGAACAA TTGAAACTGT CTTGTTTGCA GTTTATAGGA
    CTGAATATGG CCGCTTTACT TGAAGCAAGG TCTCTTGATG TTTTAAGTGA TGGTGTTTTG
    AAGGATCTTT CTGTGTTTTA TAGAAAAATG ATTCCAGCAA TGGATAGAAG AGTCATTACA
    CCATACCAAG ATGGACCAGA TATTAGCTAT TTGGAAGTAG AAGATGGAGA TGTCTTTTTG
    AAAGAAGAAA TAAATATGGA ACAAAATTAT TCGGAAAGTA TGTTCAAGAA GGCAAAAACA
    AAAGCAAAAA AGAAGCCGCG TAAGCGTTCA GATAGTTCTG GAGGTTATAA CCTTTCAGAT
    GTTATTCAGA GTCCACCATC TACAGGATTA TTAAAGTCTG GTAAGAACAA TTCTGTGGAA
    TCTCTTCCAG AACTGTTGAC ATCAGACTCT GAAGGAAGCT ATGCAGGAGT GGGTAGTCCT
    AGAGATTTAC AGTCCCCTGA TTTCACAGCA GGATTTCAGT CAGATAAAAT TGAGGTGAAA
    ATTAAACCAT ATGTTAATGG TACACCTCCT GCATATTCTA GGGAAGATTT AAAACCATGG
    GAAAAATCAC CAATACTTAA CATATCTGCT CCACAGACCA TTCCTAGTAA CAGAATTGAT
    ACTACAAACT CTTCTAGTTG GGTTGCTGGC TCTTTCAGTC CTGTCAGCCC TCCTGTTATG
    GATCTCAGAA CCATCATGCA AATTGAAGAA AGTAGACAAA AATGTGGAGC TACACCAAAG
    ACAAATTTGG GCAAGATGAT TTCTCATGGA GTTAAACTTT CTCAGAAGCA ACGGAAAATG
    ATTGCAATGA CTACCAAAGA AAACAATTCA GGAACGAATA GCATGGAAAC AGCTTTAACT
    GCTCCTTCAA AAACTCCAAA ACCAGCAAAC GCATGGGCAT CTTCTGTACA TTCAGTTTCA
    TCCAAGTCAT TCCGGGATTT CTTTGAAGAA AAAAAATCTA TTACTGGCCA TAGTTCAGGA
    GATCATGTCA AAAAAGTTTG TTTTAAAGGA ATAGAAAATT CTCAGGCATC AAAAGTTGAA
    AGATGTTCCC CTCATGGTAC GCCAGGACCA GAAGGCAACC ATATTTCAGA TTTACCACTT
    CTAGACAGTC CCAATCCCTG GCTGTCTTCT TCATTGACTG CTCCTCCTGT GGTAGCTCCA
    GTCACTTTTG CATCTATTGT AGAAGAAGAA TTACAGCAGG AAGCAGCTTT AATCAGAAGT
    CGAGAAAAAC CCTTGGCTTT GATTCAGATT GAAGAGCGTG CAATACAAGA TTTATTGGTT
    TTCTATGAGG CATTTGGCAA CCCTGATGAG TTTGTTATTG TTGAAAGGAC ACCACAGGGA
    CCACTAGCAG TACCTATGTG GAATAAGCAT GGATGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973598.1
    CDS1231..5289
    Translation

    Target ORF information:

    RefSeq Version XM_014118123.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris inhibitor of Bruton agammaglobulinemia tyrosine kinase (IBTK), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118123.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
    GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT
    TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC
    TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG
    AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT
    TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG
    TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT
    CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG
    AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG
    CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC
    TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT
    AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT
    ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA
    TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT
    CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT
    AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC
    AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC
    ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT
    ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA
    AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA
    AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG
    AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT
    TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT
    AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGCAGA GATTTTATCA
    AATCTTCATA ATTCCTCATC AGATGTGTCT TGTGTCTCTG ATATAAATAG TGTATACGAA
    AGAATTCGAC TTGAGAAACT TACTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGATCCA
    AGTGGATGCA ACTTTGCAAT CCTGCAGTCA GATCCTAAAA CAAGCCTTTA TGAAATTCCA
    GCCGTATCCT CATCATCCTT TTTTGAAGAG TTTGGCAAAC TCTTAAGGGA AACAGATGAA
    ACGGACAGTA TTCATGATGT GACATTTCAA GTTGGCAATA GAATTTTCCC TGCACATAAA
    TATATTTTGG CAGTGCGTTC TGACTTTTTT CAGAAATTGT TTCTTTCAGA TGGGACTACT
    TTAGATTTTA TAGATGTTTA CCGGAAAGAT GAAGATTCTG CAGGATGCCA TCTCTTTGTC
    ATAGAGAAGG TTCATCCTGA CTTGTTTGAG TACCTTTTAC AATTTATATA CACAGATACT
    TGTGACTTTT TAATTCATGG CTTCACACCA AGAATAAACT TAAACAAAAA GCCAGAAGAA
    TATCAGGGCA CTATGAATTC ACATTCAAAT AAAATGCATT GCCATGAAGA TAATCAGAAG
    TCAGCATTTG AAGTTTACAG AAGTAATCAA GCACACACAG TTAATGAAAA GCAAAAAAGC
    AAATCAAAGG CTTCCAATAA AGGGAAAAGT ATTGGGGAAG ATGATCCTGT AAGAATGTTG
    CAAAATGTTG CAAAGAAATT TGGTTTCAGT AATTTGAGTA GTAGGTTAGA TGGAGTCAGA
    TTTGAAAATG GAAAAATTAA TGTTATTGAG AAGAAAACTG GTAATAAATC AAAGCTAAAT
    CAGAAAAAAT GTTCCTTTCT ATGTGATGTG ACCATGAAAT CAGTGGATGG AAAGGAATTT
    CCTTGTCATA AGTGTGTTCT TTGTGCTAGA CTTGAATATT TTCATAGTAT GCTGAGTAGC
    TCATGGATTG AGGCTTCTAC TTGTACGGCT CTGGAAATGC CAATACATTC TGACATACTG
    GAAGTTATTT TGGACTACCT CTATACTGAT GAAGCTGTGG TGATAAAAGA ATCTCAAAAT
    GTGGATTTTG TTTGTAGTGT TCTCGTGGTG GCTGATCAGC TTCTTATAAC TCGATTGAAA
    GGGATTTGTG AAGTAGCATT AACTGAGAAA CTTACCCTTA AGAATGCTAC TATGGTACTG
    GAGTTTGCAG CAATGTATAA TGCTGAACAA TTGAAACTGT CTTGTTTGCA GTTTATAGGA
    CTGAATATGG CCGCTTTACT TGAAGCAAGG TCTCTTGATG TTTTAAGTGA TGGTGTTTTG
    AAGGATCTTT CTGTGTTTTA TAGAAAAATG ATTCCAGCAA TGGATAGAAG AGTCATTACA
    CCATACCAAG ATGGACCAGA TATTAGCTAT TTGGAAGTAG AAGATGGAGA TGTCTTTTTG
    AAAGAAGAAA TAAATATGGA ACAAAATTAT TCGGAAAGTA TGTTCAAGAA GGCAAAAACA
    AAAGCAAAAA AGAAGCCGCG TAAGCGTTCA GATAGTTCTG GAGGTTATAA CCTTTCAGAT
    GTTATTCAGA GTCCACCATC TACAGGATTA TTAAAGTCTG GTAAGAACAA TTCTGTGGAA
    TCTCTTCCAG AACTGTTGAC ATCAGACTCT GAAGGAAGCT ATGCAGGAGT GGGTAGTCCT
    AGAGATTTAC AGTCCCCTGA TTTCACAGCA GGATTTCAGT CAGATAAAAT TGAGGTGAAA
    ATTAAACCAT ATGTTAATGG TACACCTCCT GCATATTCTA GGGAAGATTT AAAACCATGG
    GAAAAATCAC CAATACTTAA CATATCTGCT CCACAGACCA TTCCTAGTAA CAGAATTGAT
    ACTACAAACT CTTCTAGTTG GGTTGCTGGC TCTTTCAGTC CTGTCAGCCC TCCTGTTATG
    GATCTCAGAA CCATCATGCA AATTGAAGAA AGTAGACAAA AATGTGGAGC TACACCAAAG
    ACAAATTTGG GCAAGATGAT TTCTCATGGA GTTAAACTTT CTCAGAAGCA ACGGAAAATG
    ATTGCAATGA CTACCAAAGA AAACAATTCA GGAACGAATA GCATGGAAAC AGCTTTAACT
    GCTCCTTCAA AAACTCCAAA ACCAGCAAAC GCATGGGCAT CTTCTGTACA TTCAGTTTCA
    TCCAAGTCAT TCCGGGATTT CTTTGAAGAA AAAAAATCTA TTACTGGCCA TAGTTCAGGA
    GATCATGTCA AAAAAGTTTG TTTTAAAGGA ATAGAAAATT CTCAGGCATC AAAAGTTGAA
    AGATGTTCCC CTCATGGTAC GCCAGGACCA GAAGGCAACC ATATTTCAGA TTTACCACTT
    CTAGACAGTC CCAATCCCTG GCTGTCTTCT TCATTGACTG CTCCTCCTGT GGTAGCTCCA
    GTCACTTTTG CATCTATTGT AGAAGAAGAA TTACAGCAGG AAGCAGCTTT AATCAGAAGT
    CGAGAAAAAC CCTTGGCTTT GATTCAGATT GAAGAGCGTG CAATACAAGA TTTATTGGTT
    TTCTATGAGG CATTTGGCAA CCCTGATGAG TTTGTTATTG TTGAAAGGAC ACCACAGGGA
    CCACTAGCAG TACCTATGTG GAATAAGCAT GGATGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39387
    Clone ID Related Accession (Same CDS sequence) XM_022426286.1 , XM_014118123.1 , XM_014118123.2
    Accession Version XM_014118123.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4059bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product inhibitor of Bruton tyrosine kinase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014118123.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
    GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT
    TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC
    TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG
    AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT
    TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG
    TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT
    CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG
    AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG
    CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC
    TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT
    AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT
    ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA
    TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT
    CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT
    AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC
    AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC
    ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT
    ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA
    AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA
    AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG
    AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT
    TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT
    AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGCAGA GATTTTATCA
    AATCTTCATA ATTCCTCATC AGATGTGTCT TGTGTCTCTG ATATAAATAG TGTATACGAA
    AGAATTCGAC TTGAGAAACT TACTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGATCCA
    AGTGGATGCA ACTTTGCAAT CCTGCAGTCA GATCCTAAAA CAAGCCTTTA TGAAATTCCA
    GCCGTATCCT CATCATCCTT TTTTGAAGAG TTTGGCAAAC TCTTAAGGGA AACAGATGAA
    ACGGACAGTA TTCATGATGT GACATTTCAA GTTGGCAATA GAATTTTCCC TGCACATAAA
    TATATTTTGG CAGTGCGTTC TGACTTTTTT CAGAAATTGT TTCTTTCAGA TGGGACTACT
    TTAGATTTTA TAGATGTTTA CCGGAAAGAT GAAGATTCTG CAGGATGCCA TCTCTTTGTC
    ATAGAGAAGG TTCATCCTGA CTTGTTTGAG TACCTTTTAC AATTTATATA CACAGATACT
    TGTGACTTTT TAATTCATGG CTTCACACCA AGAATAAACT TAAACAAAAA GCCAGAAGAA
    TATCAGGGCA CTATGAATTC ACATTCAAAT AAAATGCATT GCCATGAAGA TAATCAGAAG
    TCAGCATTTG AAGTTTACAG AAGTAATCAA GCACACACAG TTAATGAAAA GCAAAAAAGC
    AAATCAAAGG CTTCCAATAA AGGGAAAAGT ATTGGGGAAG ATGATCCTGT AAGAATGTTG
    CAAAATGTTG CAAAGAAATT TGGTTTCAGT AATTTGAGTA GTAGGTTAGA TGGAGTCAGA
    TTTGAAAATG GAAAAATTAA TGTTATTGAG AAGAAAACTG GTAATAAATC AAAGCTAAAT
    CAGAAAAAAT GTTCCTTTCT ATGTGATGTG ACCATGAAAT CAGTGGATGG AAAGGAATTT
    CCTTGTCATA AGTGTGTTCT TTGTGCTAGA CTTGAATATT TTCATAGTAT GCTGAGTAGC
    TCATGGATTG AGGCTTCTAC TTGTACGGCT CTGGAAATGC CAATACATTC TGACATACTG
    GAAGTTATTT TGGACTACCT CTATACTGAT GAAGCTGTGG TGATAAAAGA ATCTCAAAAT
    GTGGATTTTG TTTGTAGTGT TCTCGTGGTG GCTGATCAGC TTCTTATAAC TCGATTGAAA
    GGGATTTGTG AAGTAGCATT AACTGAGAAA CTTACCCTTA AGAATGCTAC TATGGTACTG
    GAGTTTGCAG CAATGTATAA TGCTGAACAA TTGAAACTGT CTTGTTTGCA GTTTATAGGA
    CTGAATATGG CCGCTTTACT TGAAGCAAGG TCTCTTGATG TTTTAAGTGA TGGTGTTTTG
    AAGGATCTTT CTGTGTTTTA TAGAAAAATG ATTCCAGCAA TGGATAGAAG AGTCATTACA
    CCATACCAAG ATGGACCAGA TATTAGCTAT TTGGAAGTAG AAGATGGAGA TGTCTTTTTG
    AAAGAAGAAA TAAATATGGA ACAAAATTAT TCGGAAAGTA TGTTCAAGAA GGCAAAAACA
    AAAGCAAAAA AGAAGCCGCG TAAGCGTTCA GATAGTTCTG GAGGTTATAA CCTTTCAGAT
    GTTATTCAGA GTCCACCATC TACAGGATTA TTAAAGTCTG GTAAGAACAA TTCTGTGGAA
    TCTCTTCCAG AACTGTTGAC ATCAGACTCT GAAGGAAGCT ATGCAGGAGT GGGTAGTCCT
    AGAGATTTAC AGTCCCCTGA TTTCACAGCA GGATTTCAGT CAGATAAAAT TGAGGTGAAA
    ATTAAACCAT ATGTTAATGG TACACCTCCT GCATATTCTA GGGAAGATTT AAAACCATGG
    GAAAAATCAC CAATACTTAA CATATCTGCT CCACAGACCA TTCCTAGTAA CAGAATTGAT
    ACTACAAACT CTTCTAGTTG GGTTGCTGGC TCTTTCAGTC CTGTCAGCCC TCCTGTTATG
    GATCTCAGAA CCATCATGCA AATTGAAGAA AGTAGACAAA AATGTGGAGC TACACCAAAG
    ACAAATTTGG GCAAGATGAT TTCTCATGGA GTTAAACTTT CTCAGAAGCA ACGGAAAATG
    ATTGCAATGA CTACCAAAGA AAACAATTCA GGAACGAATA GCATGGAAAC AGCTTTAACT
    GCTCCTTCAA AAACTCCAAA ACCAGCAAAC GCATGGGCAT CTTCTGTACA TTCAGTTTCA
    TCCAAGTCAT TCCGGGATTT CTTTGAAGAA AAAAAATCTA TTACTGGCCA TAGTTCAGGA
    GATCATGTCA AAAAAGTTTG TTTTAAAGGA ATAGAAAATT CTCAGGCATC AAAAGTTGAA
    AGATGTTCCC CTCATGGTAC GCCAGGACCA GAAGGCAACC ATATTTCAGA TTTACCACTT
    CTAGACAGTC CCAATCCCTG GCTGTCTTCT TCATTGACTG CTCCTCCTGT GGTAGCTCCA
    GTCACTTTTG CATCTATTGT AGAAGAAGAA TTACAGCAGG AAGCAGCTTT AATCAGAAGT
    CGAGAAAAAC CCTTGGCTTT GATTCAGATT GAAGAGCGTG CAATACAAGA TTTATTGGTT
    TTCTATGAGG CATTTGGCAA CCCTGATGAG TTTGTTATTG TTGAAAGGAC ACCACAGGGA
    CCACTAGCAG TACCTATGTG GAATAAGCAT GGATGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973598.1
    CDS556..4614
    Translation

    Target ORF information:

    RefSeq Version XM_014118123.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118123.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
    GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT
    TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC
    TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG
    AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT
    TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG
    TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT
    CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG
    AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG
    CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC
    TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT
    AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT
    ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA
    TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT
    CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT
    AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC
    AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC
    ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT
    ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA
    AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA
    AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG
    AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT
    TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT
    AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGCAGA GATTTTATCA
    AATCTTCATA ATTCCTCATC AGATGTGTCT TGTGTCTCTG ATATAAATAG TGTATACGAA
    AGAATTCGAC TTGAGAAACT TACTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGATCCA
    AGTGGATGCA ACTTTGCAAT CCTGCAGTCA GATCCTAAAA CAAGCCTTTA TGAAATTCCA
    GCCGTATCCT CATCATCCTT TTTTGAAGAG TTTGGCAAAC TCTTAAGGGA AACAGATGAA
    ACGGACAGTA TTCATGATGT GACATTTCAA GTTGGCAATA GAATTTTCCC TGCACATAAA
    TATATTTTGG CAGTGCGTTC TGACTTTTTT CAGAAATTGT TTCTTTCAGA TGGGACTACT
    TTAGATTTTA TAGATGTTTA CCGGAAAGAT GAAGATTCTG CAGGATGCCA TCTCTTTGTC
    ATAGAGAAGG TTCATCCTGA CTTGTTTGAG TACCTTTTAC AATTTATATA CACAGATACT
    TGTGACTTTT TAATTCATGG CTTCACACCA AGAATAAACT TAAACAAAAA GCCAGAAGAA
    TATCAGGGCA CTATGAATTC ACATTCAAAT AAAATGCATT GCCATGAAGA TAATCAGAAG
    TCAGCATTTG AAGTTTACAG AAGTAATCAA GCACACACAG TTAATGAAAA GCAAAAAAGC
    AAATCAAAGG CTTCCAATAA AGGGAAAAGT ATTGGGGAAG ATGATCCTGT AAGAATGTTG
    CAAAATGTTG CAAAGAAATT TGGTTTCAGT AATTTGAGTA GTAGGTTAGA TGGAGTCAGA
    TTTGAAAATG GAAAAATTAA TGTTATTGAG AAGAAAACTG GTAATAAATC AAAGCTAAAT
    CAGAAAAAAT GTTCCTTTCT ATGTGATGTG ACCATGAAAT CAGTGGATGG AAAGGAATTT
    CCTTGTCATA AGTGTGTTCT TTGTGCTAGA CTTGAATATT TTCATAGTAT GCTGAGTAGC
    TCATGGATTG AGGCTTCTAC TTGTACGGCT CTGGAAATGC CAATACATTC TGACATACTG
    GAAGTTATTT TGGACTACCT CTATACTGAT GAAGCTGTGG TGATAAAAGA ATCTCAAAAT
    GTGGATTTTG TTTGTAGTGT TCTCGTGGTG GCTGATCAGC TTCTTATAAC TCGATTGAAA
    GGGATTTGTG AAGTAGCATT AACTGAGAAA CTTACCCTTA AGAATGCTAC TATGGTACTG
    GAGTTTGCAG CAATGTATAA TGCTGAACAA TTGAAACTGT CTTGTTTGCA GTTTATAGGA
    CTGAATATGG CCGCTTTACT TGAAGCAAGG TCTCTTGATG TTTTAAGTGA TGGTGTTTTG
    AAGGATCTTT CTGTGTTTTA TAGAAAAATG ATTCCAGCAA TGGATAGAAG AGTCATTACA
    CCATACCAAG ATGGACCAGA TATTAGCTAT TTGGAAGTAG AAGATGGAGA TGTCTTTTTG
    AAAGAAGAAA TAAATATGGA ACAAAATTAT TCGGAAAGTA TGTTCAAGAA GGCAAAAACA
    AAAGCAAAAA AGAAGCCGCG TAAGCGTTCA GATAGTTCTG GAGGTTATAA CCTTTCAGAT
    GTTATTCAGA GTCCACCATC TACAGGATTA TTAAAGTCTG GTAAGAACAA TTCTGTGGAA
    TCTCTTCCAG AACTGTTGAC ATCAGACTCT GAAGGAAGCT ATGCAGGAGT GGGTAGTCCT
    AGAGATTTAC AGTCCCCTGA TTTCACAGCA GGATTTCAGT CAGATAAAAT TGAGGTGAAA
    ATTAAACCAT ATGTTAATGG TACACCTCCT GCATATTCTA GGGAAGATTT AAAACCATGG
    GAAAAATCAC CAATACTTAA CATATCTGCT CCACAGACCA TTCCTAGTAA CAGAATTGAT
    ACTACAAACT CTTCTAGTTG GGTTGCTGGC TCTTTCAGTC CTGTCAGCCC TCCTGTTATG
    GATCTCAGAA CCATCATGCA AATTGAAGAA AGTAGACAAA AATGTGGAGC TACACCAAAG
    ACAAATTTGG GCAAGATGAT TTCTCATGGA GTTAAACTTT CTCAGAAGCA ACGGAAAATG
    ATTGCAATGA CTACCAAAGA AAACAATTCA GGAACGAATA GCATGGAAAC AGCTTTAACT
    GCTCCTTCAA AAACTCCAAA ACCAGCAAAC GCATGGGCAT CTTCTGTACA TTCAGTTTCA
    TCCAAGTCAT TCCGGGATTT CTTTGAAGAA AAAAAATCTA TTACTGGCCA TAGTTCAGGA
    GATCATGTCA AAAAAGTTTG TTTTAAAGGA ATAGAAAATT CTCAGGCATC AAAAGTTGAA
    AGATGTTCCC CTCATGGTAC GCCAGGACCA GAAGGCAACC ATATTTCAGA TTTACCACTT
    CTAGACAGTC CCAATCCCTG GCTGTCTTCT TCATTGACTG CTCCTCCTGT GGTAGCTCCA
    GTCACTTTTG CATCTATTGT AGAAGAAGAA TTACAGCAGG AAGCAGCTTT AATCAGAAGT
    CGAGAAAAAC CCTTGGCTTT GATTCAGATT GAAGAGCGTG CAATACAAGA TTTATTGGTT
    TTCTATGAGG CATTTGGCAA CCCTGATGAG TTTGTTATTG TTGAAAGGAC ACCACAGGGA
    CCACTAGCAG TACCTATGTG GAATAAGCAT GGATGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe15526
    Clone ID Related Accession (Same CDS sequence) XM_005627571.2 , XM_005627571.3
    Accession Version XM_005627571.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3987bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product inhibitor of Bruton tyrosine kinase isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005627571.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
    GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT
    TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC
    TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG
    AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT
    TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG
    TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT
    CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG
    AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG
    CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC
    TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT
    AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT
    ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA
    TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT
    CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT
    AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC
    AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC
    ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT
    ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA
    AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA
    AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG
    AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT
    TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT
    AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGCAGA GATTTTATCA
    AATCTTCATA ATTCCTCATC AGATGTGTCT TGTGTCTCTG ATATAAATAG TGTATACGAA
    AGAATTCGAC TTGAGAAACT TACTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGATCCA
    AGTGGATGCA ACTTTGCAAT CCTGCAGTCA GATCCTAAAA CAAGCCTTTA TGAAATTCCA
    GCCGTATCCT CATCATCCTT TTTTGAAGAG TTTGGCAAAC TCTTAAGGGA AACAGATGAA
    ACGGACAGTA TTCATGATGT GACATTTCAA GTTGGCAATA GAATTTTCCC TGCACATAAA
    TATATTTTGG CAGTGCGTTC TGACTTTTTT CAGAAATTGT TTCTTTCAGA TGGGACTACT
    TTAGATTTTA TAGATGTTTA CCGGAAAGAT GAAGATTCTG CAGGATGCCA TCTCTTTGTC
    ATAGAGAAGG TTCATCCTGA CTTGTTTGAG TACCTTTTAC AATTTATATA CACAGATACT
    TGTGACTTTT TAATTCATGG CTTCACACCA AGAATAAACT TAAACAAAAA GCCAGAAGAA
    TATCAGGGCA CTATGAATTC ACATTCAAAT AAAATGCATT GCCATGAAGA TAATCAGAAG
    TCAGCATTTG AAGTTTACAG AAGTAATCAA GCACACACAG TTAATGAAAA GCAAAAAAGC
    AAATCAAAGG CTTCCAATAA AGGGAAAAGT ATTGGGGAAG ATGATCCTGT AAGAATGTTG
    CAAAATGTTG CAAAGAAATT TGGTTTCAGT AATTTGAGTA GTAGGTTAGA TGGAGTCAGA
    TTTGAAAATG GAAAAATTAA TGTTATTGAG AAGAAAACTG GTAATAAATC AAAGCTAAAT
    CAGAAAAAAT GTTCCTTTCT ATGTGATGTG ACCATGAAAT CAGTGGATGG AAAGGAATTT
    CCTTGTCATA AGTGTGTTCT TTGTGCTAGA CTTGAATATT TTCATAGTAT GCTGAGTAGC
    TCATGGATTG AGGCTTCTAC TTGTACGGCT CTGGAAATGC CAATACATTC TGACATACTG
    GAAGTTATTT TGGACTACCT CTATACTGAT GAAGCTGTGG TGATAAAAGA ATCTCAAAAT
    GTGGATTTTG TTTGTAGTGT TCTCGTGGTG GCTGATCAGC TTCTTATAAC TCGATTGAAA
    GGGATTTGTG AAGTAGCATT AACTGAGAAA CTTACCCTTA AGAATGCTAC TATGGTACTG
    GAGTTTGCAG CAATGTATAA TGCTGAACAA TTGAAACTGT CTTGTTTGCA GTTTATAGGA
    CTGAATATGG CCGCTTTACT TGAAGCAAGG TCTCTTGATG TTTTAAGTGA TGGTGTTTTG
    AAGGATCTTT CTGTGTTTTA TAGAAAAATG ATTCCAGCAA TGGATAGAAG AGTCATTACA
    CCATACCAAG ATGGACCAGA TATTAGCTAT TTGGAAGTAG AAGATGGAGA TGTCTTTTTG
    AAAGAAGAAA TAAATATGGA ACAAAATTAT TCGGAAAGTA TGTTCAAGAA GGCAAAAACA
    AAAGCAAAAA AGAAGCCGCG TAAGCGTTCA GATAGTTCTG GAGGTTATAA CCTTTCAGAT
    GTTATTCAGA GTCCACCATC TACAGGATTA TTAAAGTCTG GTAAGAACAA TTCTGTGGAA
    TCTCTTCCAG AACTGTTGAC ATCAGACTCT GAAGGAAGCT ATGCAGGAGT GGGTAGTCCT
    AGAGATTTAC AGTCCCCTGA TTTCACAGCA GGATTTCAGT CAGATAAAAT TGAGGTGAAA
    ATTAAACCAT ATGTTAATGG TACACCTCCT GCATATTCTA GGGAAGATTT AAAACCATGG
    GAAAAATCAC CAATACTTAA CATATCTGCT CCACAGACCA TTCCTAGTAA CAGAATTGAT
    ACTACAAACT CTTCTAGTTG GGTTGCTGGC TCTTTCAGTC CTGTCAGCCC TCCTGTTATG
    GATCTCAGAA CCATCATGCA AATTGAAGAA AGTAGACAAA AATGTGGAGC TACACCAAAG
    ACAAATTTGG GCAAGATGAT TTCTCATGGA GTTAAACTTT CTCAGAAGCA ACGGAAAATG
    ATTGCAATGA CTACCAAAGA AAACAATTCA GGAACGAATA GCATGGAAAC AGCTTTAACT
    GCTCCTTCAA AAACTCCAAA ACCAGCAAAC GCATGGGCAT CTTCTGTACA TTCAGTTTCA
    TCCAAGTCAT TCCGGGATTT CTTTGAAGAA AAAAAATCTA TTACTGGCCA TAGTTCAGGA
    GATCATGTCA AAAAAGTTTG TTTTAAAGGA ATAGAAAATT CTCAGGCATC AAAAGTTGAA
    AGTCCCTGGC TGTCTTCTTC ATTGACTGCT CCTCCTGTGG TAGCTCCAGT CACTTTTGCA
    TCTATTGTAG AAGAAGAATT ACAGCAGGAA GCAGCTTTAA TCAGAAGTCG AGAAAAACCC
    TTGGCTTTGA TTCAGATTGA AGAGCGTGCA ATACAAGATT TATTGGTTTT CTATGAGGCA
    TTTGGCAACC CTGATGAGTT TGTTATTGTT GAAAGGACAC CACAGGGACC ACTAGCAGTA
    CCTATGTGGA ATAAGCATGG ATGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005627628.1
    CDS1231..5217
    Translation

    Target ORF information:

    RefSeq Version XM_005627571.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris inhibitor of Bruton agammaglobulinemia tyrosine kinase (IBTK), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005627571.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
    GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT
    TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC
    TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG
    AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT
    TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG
    TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT
    CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG
    AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG
    CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC
    TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT
    AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT
    ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA
    TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT
    CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT
    AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC
    AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC
    ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT
    ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA
    AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA
    AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG
    AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT
    TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT
    AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGCAGA GATTTTATCA
    AATCTTCATA ATTCCTCATC AGATGTGTCT TGTGTCTCTG ATATAAATAG TGTATACGAA
    AGAATTCGAC TTGAGAAACT TACTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGATCCA
    AGTGGATGCA ACTTTGCAAT CCTGCAGTCA GATCCTAAAA CAAGCCTTTA TGAAATTCCA
    GCCGTATCCT CATCATCCTT TTTTGAAGAG TTTGGCAAAC TCTTAAGGGA AACAGATGAA
    ACGGACAGTA TTCATGATGT GACATTTCAA GTTGGCAATA GAATTTTCCC TGCACATAAA
    TATATTTTGG CAGTGCGTTC TGACTTTTTT CAGAAATTGT TTCTTTCAGA TGGGACTACT
    TTAGATTTTA TAGATGTTTA CCGGAAAGAT GAAGATTCTG CAGGATGCCA TCTCTTTGTC
    ATAGAGAAGG TTCATCCTGA CTTGTTTGAG TACCTTTTAC AATTTATATA CACAGATACT
    TGTGACTTTT TAATTCATGG CTTCACACCA AGAATAAACT TAAACAAAAA GCCAGAAGAA
    TATCAGGGCA CTATGAATTC ACATTCAAAT AAAATGCATT GCCATGAAGA TAATCAGAAG
    TCAGCATTTG AAGTTTACAG AAGTAATCAA GCACACACAG TTAATGAAAA GCAAAAAAGC
    AAATCAAAGG CTTCCAATAA AGGGAAAAGT ATTGGGGAAG ATGATCCTGT AAGAATGTTG
    CAAAATGTTG CAAAGAAATT TGGTTTCAGT AATTTGAGTA GTAGGTTAGA TGGAGTCAGA
    TTTGAAAATG GAAAAATTAA TGTTATTGAG AAGAAAACTG GTAATAAATC AAAGCTAAAT
    CAGAAAAAAT GTTCCTTTCT ATGTGATGTG ACCATGAAAT CAGTGGATGG AAAGGAATTT
    CCTTGTCATA AGTGTGTTCT TTGTGCTAGA CTTGAATATT TTCATAGTAT GCTGAGTAGC
    TCATGGATTG AGGCTTCTAC TTGTACGGCT CTGGAAATGC CAATACATTC TGACATACTG
    GAAGTTATTT TGGACTACCT CTATACTGAT GAAGCTGTGG TGATAAAAGA ATCTCAAAAT
    GTGGATTTTG TTTGTAGTGT TCTCGTGGTG GCTGATCAGC TTCTTATAAC TCGATTGAAA
    GGGATTTGTG AAGTAGCATT AACTGAGAAA CTTACCCTTA AGAATGCTAC TATGGTACTG
    GAGTTTGCAG CAATGTATAA TGCTGAACAA TTGAAACTGT CTTGTTTGCA GTTTATAGGA
    CTGAATATGG CCGCTTTACT TGAAGCAAGG TCTCTTGATG TTTTAAGTGA TGGTGTTTTG
    AAGGATCTTT CTGTGTTTTA TAGAAAAATG ATTCCAGCAA TGGATAGAAG AGTCATTACA
    CCATACCAAG ATGGACCAGA TATTAGCTAT TTGGAAGTAG AAGATGGAGA TGTCTTTTTG
    AAAGAAGAAA TAAATATGGA ACAAAATTAT TCGGAAAGTA TGTTCAAGAA GGCAAAAACA
    AAAGCAAAAA AGAAGCCGCG TAAGCGTTCA GATAGTTCTG GAGGTTATAA CCTTTCAGAT
    GTTATTCAGA GTCCACCATC TACAGGATTA TTAAAGTCTG GTAAGAACAA TTCTGTGGAA
    TCTCTTCCAG AACTGTTGAC ATCAGACTCT GAAGGAAGCT ATGCAGGAGT GGGTAGTCCT
    AGAGATTTAC AGTCCCCTGA TTTCACAGCA GGATTTCAGT CAGATAAAAT TGAGGTGAAA
    ATTAAACCAT ATGTTAATGG TACACCTCCT GCATATTCTA GGGAAGATTT AAAACCATGG
    GAAAAATCAC CAATACTTAA CATATCTGCT CCACAGACCA TTCCTAGTAA CAGAATTGAT
    ACTACAAACT CTTCTAGTTG GGTTGCTGGC TCTTTCAGTC CTGTCAGCCC TCCTGTTATG
    GATCTCAGAA CCATCATGCA AATTGAAGAA AGTAGACAAA AATGTGGAGC TACACCAAAG
    ACAAATTTGG GCAAGATGAT TTCTCATGGA GTTAAACTTT CTCAGAAGCA ACGGAAAATG
    ATTGCAATGA CTACCAAAGA AAACAATTCA GGAACGAATA GCATGGAAAC AGCTTTAACT
    GCTCCTTCAA AAACTCCAAA ACCAGCAAAC GCATGGGCAT CTTCTGTACA TTCAGTTTCA
    TCCAAGTCAT TCCGGGATTT CTTTGAAGAA AAAAAATCTA TTACTGGCCA TAGTTCAGGA
    GATCATGTCA AAAAAGTTTG TTTTAAAGGA ATAGAAAATT CTCAGGCATC AAAAGTTGAA
    AGTCCCTGGC TGTCTTCTTC ATTGACTGCT CCTCCTGTGG TAGCTCCAGT CACTTTTGCA
    TCTATTGTAG AAGAAGAATT ACAGCAGGAA GCAGCTTTAA TCAGAAGTCG AGAAAAACCC
    TTGGCTTTGA TTCAGATTGA AGAGCGTGCA ATACAAGATT TATTGGTTTT CTATGAGGCA
    TTTGGCAACC CTGATGAGTT TGTTATTGTT GAAAGGACAC CACAGGGACC ACTAGCAGTA
    CCTATGTGGA ATAAGCATGG ATGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe15526
    Clone ID Related Accession (Same CDS sequence) XM_005627571.2 , XM_005627571.3
    Accession Version XM_005627571.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3987bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product inhibitor of Bruton tyrosine kinase isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005627571.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
    GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT
    TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC
    TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG
    AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT
    TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG
    TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT
    CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG
    AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG
    CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC
    TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT
    AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT
    ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA
    TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT
    CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT
    AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC
    AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC
    ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT
    ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA
    AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA
    AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG
    AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT
    TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT
    AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGCAGA GATTTTATCA
    AATCTTCATA ATTCCTCATC AGATGTGTCT TGTGTCTCTG ATATAAATAG TGTATACGAA
    AGAATTCGAC TTGAGAAACT TACTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGATCCA
    AGTGGATGCA ACTTTGCAAT CCTGCAGTCA GATCCTAAAA CAAGCCTTTA TGAAATTCCA
    GCCGTATCCT CATCATCCTT TTTTGAAGAG TTTGGCAAAC TCTTAAGGGA AACAGATGAA
    ACGGACAGTA TTCATGATGT GACATTTCAA GTTGGCAATA GAATTTTCCC TGCACATAAA
    TATATTTTGG CAGTGCGTTC TGACTTTTTT CAGAAATTGT TTCTTTCAGA TGGGACTACT
    TTAGATTTTA TAGATGTTTA CCGGAAAGAT GAAGATTCTG CAGGATGCCA TCTCTTTGTC
    ATAGAGAAGG TTCATCCTGA CTTGTTTGAG TACCTTTTAC AATTTATATA CACAGATACT
    TGTGACTTTT TAATTCATGG CTTCACACCA AGAATAAACT TAAACAAAAA GCCAGAAGAA
    TATCAGGGCA CTATGAATTC ACATTCAAAT AAAATGCATT GCCATGAAGA TAATCAGAAG
    TCAGCATTTG AAGTTTACAG AAGTAATCAA GCACACACAG TTAATGAAAA GCAAAAAAGC
    AAATCAAAGG CTTCCAATAA AGGGAAAAGT ATTGGGGAAG ATGATCCTGT AAGAATGTTG
    CAAAATGTTG CAAAGAAATT TGGTTTCAGT AATTTGAGTA GTAGGTTAGA TGGAGTCAGA
    TTTGAAAATG GAAAAATTAA TGTTATTGAG AAGAAAACTG GTAATAAATC AAAGCTAAAT
    CAGAAAAAAT GTTCCTTTCT ATGTGATGTG ACCATGAAAT CAGTGGATGG AAAGGAATTT
    CCTTGTCATA AGTGTGTTCT TTGTGCTAGA CTTGAATATT TTCATAGTAT GCTGAGTAGC
    TCATGGATTG AGGCTTCTAC TTGTACGGCT CTGGAAATGC CAATACATTC TGACATACTG
    GAAGTTATTT TGGACTACCT CTATACTGAT GAAGCTGTGG TGATAAAAGA ATCTCAAAAT
    GTGGATTTTG TTTGTAGTGT TCTCGTGGTG GCTGATCAGC TTCTTATAAC TCGATTGAAA
    GGGATTTGTG AAGTAGCATT AACTGAGAAA CTTACCCTTA AGAATGCTAC TATGGTACTG
    GAGTTTGCAG CAATGTATAA TGCTGAACAA TTGAAACTGT CTTGTTTGCA GTTTATAGGA
    CTGAATATGG CCGCTTTACT TGAAGCAAGG TCTCTTGATG TTTTAAGTGA TGGTGTTTTG
    AAGGATCTTT CTGTGTTTTA TAGAAAAATG ATTCCAGCAA TGGATAGAAG AGTCATTACA
    CCATACCAAG ATGGACCAGA TATTAGCTAT TTGGAAGTAG AAGATGGAGA TGTCTTTTTG
    AAAGAAGAAA TAAATATGGA ACAAAATTAT TCGGAAAGTA TGTTCAAGAA GGCAAAAACA
    AAAGCAAAAA AGAAGCCGCG TAAGCGTTCA GATAGTTCTG GAGGTTATAA CCTTTCAGAT
    GTTATTCAGA GTCCACCATC TACAGGATTA TTAAAGTCTG GTAAGAACAA TTCTGTGGAA
    TCTCTTCCAG AACTGTTGAC ATCAGACTCT GAAGGAAGCT ATGCAGGAGT GGGTAGTCCT
    AGAGATTTAC AGTCCCCTGA TTTCACAGCA GGATTTCAGT CAGATAAAAT TGAGGTGAAA
    ATTAAACCAT ATGTTAATGG TACACCTCCT GCATATTCTA GGGAAGATTT AAAACCATGG
    GAAAAATCAC CAATACTTAA CATATCTGCT CCACAGACCA TTCCTAGTAA CAGAATTGAT
    ACTACAAACT CTTCTAGTTG GGTTGCTGGC TCTTTCAGTC CTGTCAGCCC TCCTGTTATG
    GATCTCAGAA CCATCATGCA AATTGAAGAA AGTAGACAAA AATGTGGAGC TACACCAAAG
    ACAAATTTGG GCAAGATGAT TTCTCATGGA GTTAAACTTT CTCAGAAGCA ACGGAAAATG
    ATTGCAATGA CTACCAAAGA AAACAATTCA GGAACGAATA GCATGGAAAC AGCTTTAACT
    GCTCCTTCAA AAACTCCAAA ACCAGCAAAC GCATGGGCAT CTTCTGTACA TTCAGTTTCA
    TCCAAGTCAT TCCGGGATTT CTTTGAAGAA AAAAAATCTA TTACTGGCCA TAGTTCAGGA
    GATCATGTCA AAAAAGTTTG TTTTAAAGGA ATAGAAAATT CTCAGGCATC AAAAGTTGAA
    AGTCCCTGGC TGTCTTCTTC ATTGACTGCT CCTCCTGTGG TAGCTCCAGT CACTTTTGCA
    TCTATTGTAG AAGAAGAATT ACAGCAGGAA GCAGCTTTAA TCAGAAGTCG AGAAAAACCC
    TTGGCTTTGA TTCAGATTGA AGAGCGTGCA ATACAAGATT TATTGGTTTT CTATGAGGCA
    TTTGGCAACC CTGATGAGTT TGTTATTGTT GAAAGGACAC CACAGGGACC ACTAGCAGTA
    CCTATGTGGA ATAAGCATGG ATGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005627628.1
    CDS556..4542
    Translation

    Target ORF information:

    RefSeq Version XM_005627571.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005627571.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
    GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT
    TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC
    TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG
    AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT
    TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG
    TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT
    CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG
    AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG
    CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC
    TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT
    AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT
    ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA
    TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT
    CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT
    AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC
    AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC
    ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT
    ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA
    AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA
    AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG
    AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT
    TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT
    AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGCAGA GATTTTATCA
    AATCTTCATA ATTCCTCATC AGATGTGTCT TGTGTCTCTG ATATAAATAG TGTATACGAA
    AGAATTCGAC TTGAGAAACT TACTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGATCCA
    AGTGGATGCA ACTTTGCAAT CCTGCAGTCA GATCCTAAAA CAAGCCTTTA TGAAATTCCA
    GCCGTATCCT CATCATCCTT TTTTGAAGAG TTTGGCAAAC TCTTAAGGGA AACAGATGAA
    ACGGACAGTA TTCATGATGT GACATTTCAA GTTGGCAATA GAATTTTCCC TGCACATAAA
    TATATTTTGG CAGTGCGTTC TGACTTTTTT CAGAAATTGT TTCTTTCAGA TGGGACTACT
    TTAGATTTTA TAGATGTTTA CCGGAAAGAT GAAGATTCTG CAGGATGCCA TCTCTTTGTC
    ATAGAGAAGG TTCATCCTGA CTTGTTTGAG TACCTTTTAC AATTTATATA CACAGATACT
    TGTGACTTTT TAATTCATGG CTTCACACCA AGAATAAACT TAAACAAAAA GCCAGAAGAA
    TATCAGGGCA CTATGAATTC ACATTCAAAT AAAATGCATT GCCATGAAGA TAATCAGAAG
    TCAGCATTTG AAGTTTACAG AAGTAATCAA GCACACACAG TTAATGAAAA GCAAAAAAGC
    AAATCAAAGG CTTCCAATAA AGGGAAAAGT ATTGGGGAAG ATGATCCTGT AAGAATGTTG
    CAAAATGTTG CAAAGAAATT TGGTTTCAGT AATTTGAGTA GTAGGTTAGA TGGAGTCAGA
    TTTGAAAATG GAAAAATTAA TGTTATTGAG AAGAAAACTG GTAATAAATC AAAGCTAAAT
    CAGAAAAAAT GTTCCTTTCT ATGTGATGTG ACCATGAAAT CAGTGGATGG AAAGGAATTT
    CCTTGTCATA AGTGTGTTCT TTGTGCTAGA CTTGAATATT TTCATAGTAT GCTGAGTAGC
    TCATGGATTG AGGCTTCTAC TTGTACGGCT CTGGAAATGC CAATACATTC TGACATACTG
    GAAGTTATTT TGGACTACCT CTATACTGAT GAAGCTGTGG TGATAAAAGA ATCTCAAAAT
    GTGGATTTTG TTTGTAGTGT TCTCGTGGTG GCTGATCAGC TTCTTATAAC TCGATTGAAA
    GGGATTTGTG AAGTAGCATT AACTGAGAAA CTTACCCTTA AGAATGCTAC TATGGTACTG
    GAGTTTGCAG CAATGTATAA TGCTGAACAA TTGAAACTGT CTTGTTTGCA GTTTATAGGA
    CTGAATATGG CCGCTTTACT TGAAGCAAGG TCTCTTGATG TTTTAAGTGA TGGTGTTTTG
    AAGGATCTTT CTGTGTTTTA TAGAAAAATG ATTCCAGCAA TGGATAGAAG AGTCATTACA
    CCATACCAAG ATGGACCAGA TATTAGCTAT TTGGAAGTAG AAGATGGAGA TGTCTTTTTG
    AAAGAAGAAA TAAATATGGA ACAAAATTAT TCGGAAAGTA TGTTCAAGAA GGCAAAAACA
    AAAGCAAAAA AGAAGCCGCG TAAGCGTTCA GATAGTTCTG GAGGTTATAA CCTTTCAGAT
    GTTATTCAGA GTCCACCATC TACAGGATTA TTAAAGTCTG GTAAGAACAA TTCTGTGGAA
    TCTCTTCCAG AACTGTTGAC ATCAGACTCT GAAGGAAGCT ATGCAGGAGT GGGTAGTCCT
    AGAGATTTAC AGTCCCCTGA TTTCACAGCA GGATTTCAGT CAGATAAAAT TGAGGTGAAA
    ATTAAACCAT ATGTTAATGG TACACCTCCT GCATATTCTA GGGAAGATTT AAAACCATGG
    GAAAAATCAC CAATACTTAA CATATCTGCT CCACAGACCA TTCCTAGTAA CAGAATTGAT
    ACTACAAACT CTTCTAGTTG GGTTGCTGGC TCTTTCAGTC CTGTCAGCCC TCCTGTTATG
    GATCTCAGAA CCATCATGCA AATTGAAGAA AGTAGACAAA AATGTGGAGC TACACCAAAG
    ACAAATTTGG GCAAGATGAT TTCTCATGGA GTTAAACTTT CTCAGAAGCA ACGGAAAATG
    ATTGCAATGA CTACCAAAGA AAACAATTCA GGAACGAATA GCATGGAAAC AGCTTTAACT
    GCTCCTTCAA AAACTCCAAA ACCAGCAAAC GCATGGGCAT CTTCTGTACA TTCAGTTTCA
    TCCAAGTCAT TCCGGGATTT CTTTGAAGAA AAAAAATCTA TTACTGGCCA TAGTTCAGGA
    GATCATGTCA AAAAAGTTTG TTTTAAAGGA ATAGAAAATT CTCAGGCATC AAAAGTTGAA
    AGTCCCTGGC TGTCTTCTTC ATTGACTGCT CCTCCTGTGG TAGCTCCAGT CACTTTTGCA
    TCTATTGTAG AAGAAGAATT ACAGCAGGAA GCAGCTTTAA TCAGAAGTCG AGAAAAACCC
    TTGGCTTTGA TTCAGATTGA AGAGCGTGCA ATACAAGATT TATTGGTTTT CTATGAGGCA
    TTTGGCAACC CTGATGAGTT TGTTATTGTT GAAAGGACAC CACAGGGACC ACTAGCAGTA
    CCTATGTGGA ATAAGCATGG ATGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe15523
    Clone ID Related Accession (Same CDS sequence) XM_539018.5 , XM_539018.6
    Accession Version XM_539018.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4056bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product inhibitor of Bruton tyrosine kinase isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_539018.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
    GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT
    TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC
    TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG
    AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT
    TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG
    TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT
    CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG
    AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG
    CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC
    TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT
    AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT
    ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA
    TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT
    CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT
    AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC
    AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC
    ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT
    ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA
    AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA
    AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG
    AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT
    TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT
    AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGAGAT TTTATCAAAT
    CTTCATAATT CCTCATCAGA TGTGTCTTGT GTCTCTGATA TAAATAGTGT ATACGAAAGA
    ATTCGACTTG AGAAACTTAC TTTTGCCCAT AGAGCTGTTA GTGTCAGCAC AGATCCAAGT
    GGATGCAACT TTGCAATCCT GCAGTCAGAT CCTAAAACAA GCCTTTATGA AATTCCAGCC
    GTATCCTCAT CATCCTTTTT TGAAGAGTTT GGCAAACTCT TAAGGGAAAC AGATGAAACG
    GACAGTATTC ATGATGTGAC ATTTCAAGTT GGCAATAGAA TTTTCCCTGC ACATAAATAT
    ATTTTGGCAG TGCGTTCTGA CTTTTTTCAG AAATTGTTTC TTTCAGATGG GACTACTTTA
    GATTTTATAG ATGTTTACCG GAAAGATGAA GATTCTGCAG GATGCCATCT CTTTGTCATA
    GAGAAGGTTC ATCCTGACTT GTTTGAGTAC CTTTTACAAT TTATATACAC AGATACTTGT
    GACTTTTTAA TTCATGGCTT CACACCAAGA ATAAACTTAA ACAAAAAGCC AGAAGAATAT
    CAGGGCACTA TGAATTCACA TTCAAATAAA ATGCATTGCC ATGAAGATAA TCAGAAGTCA
    GCATTTGAAG TTTACAGAAG TAATCAAGCA CACACAGTTA ATGAAAAGCA AAAAAGCAAA
    TCAAAGGCTT CCAATAAAGG GAAAAGTATT GGGGAAGATG ATCCTGTAAG AATGTTGCAA
    AATGTTGCAA AGAAATTTGG TTTCAGTAAT TTGAGTAGTA GGTTAGATGG AGTCAGATTT
    GAAAATGGAA AAATTAATGT TATTGAGAAG AAAACTGGTA ATAAATCAAA GCTAAATCAG
    AAAAAATGTT CCTTTCTATG TGATGTGACC ATGAAATCAG TGGATGGAAA GGAATTTCCT
    TGTCATAAGT GTGTTCTTTG TGCTAGACTT GAATATTTTC ATAGTATGCT GAGTAGCTCA
    TGGATTGAGG CTTCTACTTG TACGGCTCTG GAAATGCCAA TACATTCTGA CATACTGGAA
    GTTATTTTGG ACTACCTCTA TACTGATGAA GCTGTGGTGA TAAAAGAATC TCAAAATGTG
    GATTTTGTTT GTAGTGTTCT CGTGGTGGCT GATCAGCTTC TTATAACTCG ATTGAAAGGG
    ATTTGTGAAG TAGCATTAAC TGAGAAACTT ACCCTTAAGA ATGCTACTAT GGTACTGGAG
    TTTGCAGCAA TGTATAATGC TGAACAATTG AAACTGTCTT GTTTGCAGTT TATAGGACTG
    AATATGGCCG CTTTACTTGA AGCAAGGTCT CTTGATGTTT TAAGTGATGG TGTTTTGAAG
    GATCTTTCTG TGTTTTATAG AAAAATGATT CCAGCAATGG ATAGAAGAGT CATTACACCA
    TACCAAGATG GACCAGATAT TAGCTATTTG GAAGTAGAAG ATGGAGATGT CTTTTTGAAA
    GAAGAAATAA ATATGGAACA AAATTATTCG GAAAGTATGT TCAAGAAGGC AAAAACAAAA
    GCAAAAAAGA AGCCGCGTAA GCGTTCAGAT AGTTCTGGAG GTTATAACCT TTCAGATGTT
    ATTCAGAGTC CACCATCTAC AGGATTATTA AAGTCTGGTA AGAACAATTC TGTGGAATCT
    CTTCCAGAAC TGTTGACATC AGACTCTGAA GGAAGCTATG CAGGAGTGGG TAGTCCTAGA
    GATTTACAGT CCCCTGATTT CACAGCAGGA TTTCAGTCAG ATAAAATTGA GGTGAAAATT
    AAACCATATG TTAATGGTAC ACCTCCTGCA TATTCTAGGG AAGATTTAAA ACCATGGGAA
    AAATCACCAA TACTTAACAT ATCTGCTCCA CAGACCATTC CTAGTAACAG AATTGATACT
    ACAAACTCTT CTAGTTGGGT TGCTGGCTCT TTCAGTCCTG TCAGCCCTCC TGTTATGGAT
    CTCAGAACCA TCATGCAAAT TGAAGAAAGT AGACAAAAAT GTGGAGCTAC ACCAAAGACA
    AATTTGGGCA AGATGATTTC TCATGGAGTT AAACTTTCTC AGAAGCAACG GAAAATGATT
    GCAATGACTA CCAAAGAAAA CAATTCAGGA ACGAATAGCA TGGAAACAGC TTTAACTGCT
    CCTTCAAAAA CTCCAAAACC AGCAAACGCA TGGGCATCTT CTGTACATTC AGTTTCATCC
    AAGTCATTCC GGGATTTCTT TGAAGAAAAA AAATCTATTA CTGGCCATAG TTCAGGAGAT
    CATGTCAAAA AAGTTTGTTT TAAAGGAATA GAAAATTCTC AGGCATCAAA AGTTGAAAGA
    TGTTCCCCTC ATGGTACGCC AGGACCAGAA GGCAACCATA TTTCAGATTT ACCACTTCTA
    GACAGTCCCA ATCCCTGGCT GTCTTCTTCA TTGACTGCTC CTCCTGTGGT AGCTCCAGTC
    ACTTTTGCAT CTATTGTAGA AGAAGAATTA CAGCAGGAAG CAGCTTTAAT CAGAAGTCGA
    GAAAAACCCT TGGCTTTGAT TCAGATTGAA GAGCGTGCAA TACAAGATTT ATTGGTTTTC
    TATGAGGCAT TTGGCAACCC TGATGAGTTT GTTATTGTTG AAAGGACACC ACAGGGACCA
    CTAGCAGTAC CTATGTGGAA TAAGCATGGA TGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_539018.2
    CDS1231..5286
    Translation

    Target ORF information:

    RefSeq Version XM_539018.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris inhibitor of Bruton agammaglobulinemia tyrosine kinase (IBTK), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_539018.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
    GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT
    TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC
    TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG
    AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT
    TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG
    TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT
    CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG
    AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG
    CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC
    TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT
    AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT
    ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA
    TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT
    CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT
    AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC
    AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC
    ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT
    ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA
    AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA
    AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG
    AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT
    TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT
    AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGAGAT TTTATCAAAT
    CTTCATAATT CCTCATCAGA TGTGTCTTGT GTCTCTGATA TAAATAGTGT ATACGAAAGA
    ATTCGACTTG AGAAACTTAC TTTTGCCCAT AGAGCTGTTA GTGTCAGCAC AGATCCAAGT
    GGATGCAACT TTGCAATCCT GCAGTCAGAT CCTAAAACAA GCCTTTATGA AATTCCAGCC
    GTATCCTCAT CATCCTTTTT TGAAGAGTTT GGCAAACTCT TAAGGGAAAC AGATGAAACG
    GACAGTATTC ATGATGTGAC ATTTCAAGTT GGCAATAGAA TTTTCCCTGC ACATAAATAT
    ATTTTGGCAG TGCGTTCTGA CTTTTTTCAG AAATTGTTTC TTTCAGATGG GACTACTTTA
    GATTTTATAG ATGTTTACCG GAAAGATGAA GATTCTGCAG GATGCCATCT CTTTGTCATA
    GAGAAGGTTC ATCCTGACTT GTTTGAGTAC CTTTTACAAT TTATATACAC AGATACTTGT
    GACTTTTTAA TTCATGGCTT CACACCAAGA ATAAACTTAA ACAAAAAGCC AGAAGAATAT
    CAGGGCACTA TGAATTCACA TTCAAATAAA ATGCATTGCC ATGAAGATAA TCAGAAGTCA
    GCATTTGAAG TTTACAGAAG TAATCAAGCA CACACAGTTA ATGAAAAGCA AAAAAGCAAA
    TCAAAGGCTT CCAATAAAGG GAAAAGTATT GGGGAAGATG ATCCTGTAAG AATGTTGCAA
    AATGTTGCAA AGAAATTTGG TTTCAGTAAT TTGAGTAGTA GGTTAGATGG AGTCAGATTT
    GAAAATGGAA AAATTAATGT TATTGAGAAG AAAACTGGTA ATAAATCAAA GCTAAATCAG
    AAAAAATGTT CCTTTCTATG TGATGTGACC ATGAAATCAG TGGATGGAAA GGAATTTCCT
    TGTCATAAGT GTGTTCTTTG TGCTAGACTT GAATATTTTC ATAGTATGCT GAGTAGCTCA
    TGGATTGAGG CTTCTACTTG TACGGCTCTG GAAATGCCAA TACATTCTGA CATACTGGAA
    GTTATTTTGG ACTACCTCTA TACTGATGAA GCTGTGGTGA TAAAAGAATC TCAAAATGTG
    GATTTTGTTT GTAGTGTTCT CGTGGTGGCT GATCAGCTTC TTATAACTCG ATTGAAAGGG
    ATTTGTGAAG TAGCATTAAC TGAGAAACTT ACCCTTAAGA ATGCTACTAT GGTACTGGAG
    TTTGCAGCAA TGTATAATGC TGAACAATTG AAACTGTCTT GTTTGCAGTT TATAGGACTG
    AATATGGCCG CTTTACTTGA AGCAAGGTCT CTTGATGTTT TAAGTGATGG TGTTTTGAAG
    GATCTTTCTG TGTTTTATAG AAAAATGATT CCAGCAATGG ATAGAAGAGT CATTACACCA
    TACCAAGATG GACCAGATAT TAGCTATTTG GAAGTAGAAG ATGGAGATGT CTTTTTGAAA
    GAAGAAATAA ATATGGAACA AAATTATTCG GAAAGTATGT TCAAGAAGGC AAAAACAAAA
    GCAAAAAAGA AGCCGCGTAA GCGTTCAGAT AGTTCTGGAG GTTATAACCT TTCAGATGTT
    ATTCAGAGTC CACCATCTAC AGGATTATTA AAGTCTGGTA AGAACAATTC TGTGGAATCT
    CTTCCAGAAC TGTTGACATC AGACTCTGAA GGAAGCTATG CAGGAGTGGG TAGTCCTAGA
    GATTTACAGT CCCCTGATTT CACAGCAGGA TTTCAGTCAG ATAAAATTGA GGTGAAAATT
    AAACCATATG TTAATGGTAC ACCTCCTGCA TATTCTAGGG AAGATTTAAA ACCATGGGAA
    AAATCACCAA TACTTAACAT ATCTGCTCCA CAGACCATTC CTAGTAACAG AATTGATACT
    ACAAACTCTT CTAGTTGGGT TGCTGGCTCT TTCAGTCCTG TCAGCCCTCC TGTTATGGAT
    CTCAGAACCA TCATGCAAAT TGAAGAAAGT AGACAAAAAT GTGGAGCTAC ACCAAAGACA
    AATTTGGGCA AGATGATTTC TCATGGAGTT AAACTTTCTC AGAAGCAACG GAAAATGATT
    GCAATGACTA CCAAAGAAAA CAATTCAGGA ACGAATAGCA TGGAAACAGC TTTAACTGCT
    CCTTCAAAAA CTCCAAAACC AGCAAACGCA TGGGCATCTT CTGTACATTC AGTTTCATCC
    AAGTCATTCC GGGATTTCTT TGAAGAAAAA AAATCTATTA CTGGCCATAG TTCAGGAGAT
    CATGTCAAAA AAGTTTGTTT TAAAGGAATA GAAAATTCTC AGGCATCAAA AGTTGAAAGA
    TGTTCCCCTC ATGGTACGCC AGGACCAGAA GGCAACCATA TTTCAGATTT ACCACTTCTA
    GACAGTCCCA ATCCCTGGCT GTCTTCTTCA TTGACTGCTC CTCCTGTGGT AGCTCCAGTC
    ACTTTTGCAT CTATTGTAGA AGAAGAATTA CAGCAGGAAG CAGCTTTAAT CAGAAGTCGA
    GAAAAACCCT TGGCTTTGAT TCAGATTGAA GAGCGTGCAA TACAAGATTT ATTGGTTTTC
    TATGAGGCAT TTGGCAACCC TGATGAGTTT GTTATTGTTG AAAGGACACC ACAGGGACCA
    CTAGCAGTAC CTATGTGGAA TAAGCATGGA TGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe15523
    Clone ID Related Accession (Same CDS sequence) XM_539018.5 , XM_539018.6
    Accession Version XM_539018.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4056bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product inhibitor of Bruton tyrosine kinase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_539018.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
    GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT
    TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC
    TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG
    AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT
    TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG
    TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT
    CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG
    AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG
    CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC
    TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT
    AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT
    ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA
    TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT
    CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT
    AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC
    AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC
    ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT
    ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA
    AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA
    AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG
    AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT
    TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT
    AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGAGAT TTTATCAAAT
    CTTCATAATT CCTCATCAGA TGTGTCTTGT GTCTCTGATA TAAATAGTGT ATACGAAAGA
    ATTCGACTTG AGAAACTTAC TTTTGCCCAT AGAGCTGTTA GTGTCAGCAC AGATCCAAGT
    GGATGCAACT TTGCAATCCT GCAGTCAGAT CCTAAAACAA GCCTTTATGA AATTCCAGCC
    GTATCCTCAT CATCCTTTTT TGAAGAGTTT GGCAAACTCT TAAGGGAAAC AGATGAAACG
    GACAGTATTC ATGATGTGAC ATTTCAAGTT GGCAATAGAA TTTTCCCTGC ACATAAATAT
    ATTTTGGCAG TGCGTTCTGA CTTTTTTCAG AAATTGTTTC TTTCAGATGG GACTACTTTA
    GATTTTATAG ATGTTTACCG GAAAGATGAA GATTCTGCAG GATGCCATCT CTTTGTCATA
    GAGAAGGTTC ATCCTGACTT GTTTGAGTAC CTTTTACAAT TTATATACAC AGATACTTGT
    GACTTTTTAA TTCATGGCTT CACACCAAGA ATAAACTTAA ACAAAAAGCC AGAAGAATAT
    CAGGGCACTA TGAATTCACA TTCAAATAAA ATGCATTGCC ATGAAGATAA TCAGAAGTCA
    GCATTTGAAG TTTACAGAAG TAATCAAGCA CACACAGTTA ATGAAAAGCA AAAAAGCAAA
    TCAAAGGCTT CCAATAAAGG GAAAAGTATT GGGGAAGATG ATCCTGTAAG AATGTTGCAA
    AATGTTGCAA AGAAATTTGG TTTCAGTAAT TTGAGTAGTA GGTTAGATGG AGTCAGATTT
    GAAAATGGAA AAATTAATGT TATTGAGAAG AAAACTGGTA ATAAATCAAA GCTAAATCAG
    AAAAAATGTT CCTTTCTATG TGATGTGACC ATGAAATCAG TGGATGGAAA GGAATTTCCT
    TGTCATAAGT GTGTTCTTTG TGCTAGACTT GAATATTTTC ATAGTATGCT GAGTAGCTCA
    TGGATTGAGG CTTCTACTTG TACGGCTCTG GAAATGCCAA TACATTCTGA CATACTGGAA
    GTTATTTTGG ACTACCTCTA TACTGATGAA GCTGTGGTGA TAAAAGAATC TCAAAATGTG
    GATTTTGTTT GTAGTGTTCT CGTGGTGGCT GATCAGCTTC TTATAACTCG ATTGAAAGGG
    ATTTGTGAAG TAGCATTAAC TGAGAAACTT ACCCTTAAGA ATGCTACTAT GGTACTGGAG
    TTTGCAGCAA TGTATAATGC TGAACAATTG AAACTGTCTT GTTTGCAGTT TATAGGACTG
    AATATGGCCG CTTTACTTGA AGCAAGGTCT CTTGATGTTT TAAGTGATGG TGTTTTGAAG
    GATCTTTCTG TGTTTTATAG AAAAATGATT CCAGCAATGG ATAGAAGAGT CATTACACCA
    TACCAAGATG GACCAGATAT TAGCTATTTG GAAGTAGAAG ATGGAGATGT CTTTTTGAAA
    GAAGAAATAA ATATGGAACA AAATTATTCG GAAAGTATGT TCAAGAAGGC AAAAACAAAA
    GCAAAAAAGA AGCCGCGTAA GCGTTCAGAT AGTTCTGGAG GTTATAACCT TTCAGATGTT
    ATTCAGAGTC CACCATCTAC AGGATTATTA AAGTCTGGTA AGAACAATTC TGTGGAATCT
    CTTCCAGAAC TGTTGACATC AGACTCTGAA GGAAGCTATG CAGGAGTGGG TAGTCCTAGA
    GATTTACAGT CCCCTGATTT CACAGCAGGA TTTCAGTCAG ATAAAATTGA GGTGAAAATT
    AAACCATATG TTAATGGTAC ACCTCCTGCA TATTCTAGGG AAGATTTAAA ACCATGGGAA
    AAATCACCAA TACTTAACAT ATCTGCTCCA CAGACCATTC CTAGTAACAG AATTGATACT
    ACAAACTCTT CTAGTTGGGT TGCTGGCTCT TTCAGTCCTG TCAGCCCTCC TGTTATGGAT
    CTCAGAACCA TCATGCAAAT TGAAGAAAGT AGACAAAAAT GTGGAGCTAC ACCAAAGACA
    AATTTGGGCA AGATGATTTC TCATGGAGTT AAACTTTCTC AGAAGCAACG GAAAATGATT
    GCAATGACTA CCAAAGAAAA CAATTCAGGA ACGAATAGCA TGGAAACAGC TTTAACTGCT
    CCTTCAAAAA CTCCAAAACC AGCAAACGCA TGGGCATCTT CTGTACATTC AGTTTCATCC
    AAGTCATTCC GGGATTTCTT TGAAGAAAAA AAATCTATTA CTGGCCATAG TTCAGGAGAT
    CATGTCAAAA AAGTTTGTTT TAAAGGAATA GAAAATTCTC AGGCATCAAA AGTTGAAAGA
    TGTTCCCCTC ATGGTACGCC AGGACCAGAA GGCAACCATA TTTCAGATTT ACCACTTCTA
    GACAGTCCCA ATCCCTGGCT GTCTTCTTCA TTGACTGCTC CTCCTGTGGT AGCTCCAGTC
    ACTTTTGCAT CTATTGTAGA AGAAGAATTA CAGCAGGAAG CAGCTTTAAT CAGAAGTCGA
    GAAAAACCCT TGGCTTTGAT TCAGATTGAA GAGCGTGCAA TACAAGATTT ATTGGTTTTC
    TATGAGGCAT TTGGCAACCC TGATGAGTTT GTTATTGTTG AAAGGACACC ACAGGGACCA
    CTAGCAGTAC CTATGTGGAA TAAGCATGGA TGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_539018.2
    CDS556..4611
    Translation

    Target ORF information:

    RefSeq Version XM_539018.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_539018.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
    GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT
    TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC
    TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG
    AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT
    TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG
    TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT
    CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG
    AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG
    CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC
    TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT
    AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT
    ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA
    TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT
    CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT
    AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC
    AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC
    ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT
    ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA
    AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA
    AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG
    AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT
    TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT
    AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGAGAT TTTATCAAAT
    CTTCATAATT CCTCATCAGA TGTGTCTTGT GTCTCTGATA TAAATAGTGT ATACGAAAGA
    ATTCGACTTG AGAAACTTAC TTTTGCCCAT AGAGCTGTTA GTGTCAGCAC AGATCCAAGT
    GGATGCAACT TTGCAATCCT GCAGTCAGAT CCTAAAACAA GCCTTTATGA AATTCCAGCC
    GTATCCTCAT CATCCTTTTT TGAAGAGTTT GGCAAACTCT TAAGGGAAAC AGATGAAACG
    GACAGTATTC ATGATGTGAC ATTTCAAGTT GGCAATAGAA TTTTCCCTGC ACATAAATAT
    ATTTTGGCAG TGCGTTCTGA CTTTTTTCAG AAATTGTTTC TTTCAGATGG GACTACTTTA
    GATTTTATAG ATGTTTACCG GAAAGATGAA GATTCTGCAG GATGCCATCT CTTTGTCATA
    GAGAAGGTTC ATCCTGACTT GTTTGAGTAC CTTTTACAAT TTATATACAC AGATACTTGT
    GACTTTTTAA TTCATGGCTT CACACCAAGA ATAAACTTAA ACAAAAAGCC AGAAGAATAT
    CAGGGCACTA TGAATTCACA TTCAAATAAA ATGCATTGCC ATGAAGATAA TCAGAAGTCA
    GCATTTGAAG TTTACAGAAG TAATCAAGCA CACACAGTTA ATGAAAAGCA AAAAAGCAAA
    TCAAAGGCTT CCAATAAAGG GAAAAGTATT GGGGAAGATG ATCCTGTAAG AATGTTGCAA
    AATGTTGCAA AGAAATTTGG TTTCAGTAAT TTGAGTAGTA GGTTAGATGG AGTCAGATTT
    GAAAATGGAA AAATTAATGT TATTGAGAAG AAAACTGGTA ATAAATCAAA GCTAAATCAG
    AAAAAATGTT CCTTTCTATG TGATGTGACC ATGAAATCAG TGGATGGAAA GGAATTTCCT
    TGTCATAAGT GTGTTCTTTG TGCTAGACTT GAATATTTTC ATAGTATGCT GAGTAGCTCA
    TGGATTGAGG CTTCTACTTG TACGGCTCTG GAAATGCCAA TACATTCTGA CATACTGGAA
    GTTATTTTGG ACTACCTCTA TACTGATGAA GCTGTGGTGA TAAAAGAATC TCAAAATGTG
    GATTTTGTTT GTAGTGTTCT CGTGGTGGCT GATCAGCTTC TTATAACTCG ATTGAAAGGG
    ATTTGTGAAG TAGCATTAAC TGAGAAACTT ACCCTTAAGA ATGCTACTAT GGTACTGGAG
    TTTGCAGCAA TGTATAATGC TGAACAATTG AAACTGTCTT GTTTGCAGTT TATAGGACTG
    AATATGGCCG CTTTACTTGA AGCAAGGTCT CTTGATGTTT TAAGTGATGG TGTTTTGAAG
    GATCTTTCTG TGTTTTATAG AAAAATGATT CCAGCAATGG ATAGAAGAGT CATTACACCA
    TACCAAGATG GACCAGATAT TAGCTATTTG GAAGTAGAAG ATGGAGATGT CTTTTTGAAA
    GAAGAAATAA ATATGGAACA AAATTATTCG GAAAGTATGT TCAAGAAGGC AAAAACAAAA
    GCAAAAAAGA AGCCGCGTAA GCGTTCAGAT AGTTCTGGAG GTTATAACCT TTCAGATGTT
    ATTCAGAGTC CACCATCTAC AGGATTATTA AAGTCTGGTA AGAACAATTC TGTGGAATCT
    CTTCCAGAAC TGTTGACATC AGACTCTGAA GGAAGCTATG CAGGAGTGGG TAGTCCTAGA
    GATTTACAGT CCCCTGATTT CACAGCAGGA TTTCAGTCAG ATAAAATTGA GGTGAAAATT
    AAACCATATG TTAATGGTAC ACCTCCTGCA TATTCTAGGG AAGATTTAAA ACCATGGGAA
    AAATCACCAA TACTTAACAT ATCTGCTCCA CAGACCATTC CTAGTAACAG AATTGATACT
    ACAAACTCTT CTAGTTGGGT TGCTGGCTCT TTCAGTCCTG TCAGCCCTCC TGTTATGGAT
    CTCAGAACCA TCATGCAAAT TGAAGAAAGT AGACAAAAAT GTGGAGCTAC ACCAAAGACA
    AATTTGGGCA AGATGATTTC TCATGGAGTT AAACTTTCTC AGAAGCAACG GAAAATGATT
    GCAATGACTA CCAAAGAAAA CAATTCAGGA ACGAATAGCA TGGAAACAGC TTTAACTGCT
    CCTTCAAAAA CTCCAAAACC AGCAAACGCA TGGGCATCTT CTGTACATTC AGTTTCATCC
    AAGTCATTCC GGGATTTCTT TGAAGAAAAA AAATCTATTA CTGGCCATAG TTCAGGAGAT
    CATGTCAAAA AAGTTTGTTT TAAAGGAATA GAAAATTCTC AGGCATCAAA AGTTGAAAGA
    TGTTCCCCTC ATGGTACGCC AGGACCAGAA GGCAACCATA TTTCAGATTT ACCACTTCTA
    GACAGTCCCA ATCCCTGGCT GTCTTCTTCA TTGACTGCTC CTCCTGTGGT AGCTCCAGTC
    ACTTTTGCAT CTATTGTAGA AGAAGAATTA CAGCAGGAAG CAGCTTTAAT CAGAAGTCGA
    GAAAAACCCT TGGCTTTGAT TCAGATTGAA GAGCGTGCAA TACAAGATTT ATTGGTTTTC
    TATGAGGCAT TTGGCAACCC TGATGAGTTT GTTATTGTTG AAAGGACACC ACAGGGACCA
    CTAGCAGTAC CTATGTGGAA TAAGCATGGA TGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe15524
    Clone ID Related Accession (Same CDS sequence) XM_005627569.3 , XM_005627569.2
    Accession Version XM_005627569.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4014bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product inhibitor of Bruton tyrosine kinase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005627569.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
    GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT
    TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC
    TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG
    AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT
    TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG
    TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT
    CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG
    AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG
    CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC
    TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT
    AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT
    ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA
    TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT
    CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT
    AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC
    AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC
    ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT
    ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA
    AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA
    AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG
    AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT
    TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT
    AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGCAGA GATTTTATCA
    AATCTTCATA ATTCCTCATC AGATGTGTCT TGTGTCTCTG ATATAAATAG TGTATACGAA
    AGAATTCGAC TTGAGAAACT TACTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGATCCA
    AGTGGATGCA ACTTTGCAAT CCTGCAGTCA GATCCTAAAA CAAGCCTTTA TGAAATTCCA
    GCCGTATCCT CATCATCCTT TTTTGAAGAG TTTGGCAAAC TCTTAAGGGA AACAGATGAA
    ACGGACAGTA TTCATGATGT GACATTTCAA GTTGGCAATA GAATTTTCCC TGCACATAAA
    TATATTTTGG CAGTGCGTTC TGACTTTTTT CAGAAATTGT TTCTTTCAGA TGGGACTACT
    TTAGATTTTA TAGATGTTTA CCGGAAAGAT GAAGATTCTG CAGGATGCCA TCTCTTTGTC
    ATAGAGAAGG TTCATCCTGA CTTGTTTGAG TACCTTTTAC AATTTATATA CACAGATACT
    TGTGACTTTT TAATTCATGG CTTCACACCA AGAATAAACT TAAACAAAAA GCCAGAAGAA
    TATCAGGGCA CTATGAATTC ACATTCAAAT AAAATGCATT GCCATGAAGA TAATCAGAAG
    TCAGCATTTG AAGTTTACAG AAGTAATCAA GCACACACAG TTAATGAAAA GCAAAAAAGC
    AAATCAAAGG CTTCCAATAA AGGGAAAAGT ATTGGGGAAG ATGATCCTGT AAGAATGTTG
    CAAAATGTTG CAAAGAAATT TGGTTTCAGT AATTTGAGTA GTAGGTTAGA TGGAGTCAGA
    TTTGAAAATG GAAAAATTAA TGTTATTGAG AAGAAAACTG GTAATAAATC AAAGCTAAAT
    CAGAAAAAAT GTTCCTTTCT ATGTGATGTG ACCATGAAAT CAGTGGATGG AAAGGAATTT
    CCTTGTCATA AGTGTGTTCT TTGTGCTAGA CTTGAATATT TTCATAGTAT GCTGAGTAGC
    TCATGGATTG AGGCTTCTAC TTGTACGGCT CTGGAAATGC CAATACATTC TGACATACTG
    GAAGTTATTT TGGACTACCT CTATACTGAT GAAGCTGTGG TGATAAAAGA ATCTCAAAAT
    GTGGATTTTG TTTGTAGTGT TCTCGTGGTG GCTGATCAGC TTCTTATAAC TCGATTGAAA
    GGGATTTGTG AAGTAGCATT AACTGAGAAA CTTACCCTTA AGAATGCTAC TATGGTACTG
    GAGTTTGCAG CAATGTATAA TGCTGAACAA TTGAAACTGT CTTGTTTGCA GTTTATAGGA
    CTGAATATGG CCGCTTTACT TGAAGCAAGG TCTCTTGATG TTTTAAGTGA TGGTGTTTTG
    AAGGATCTTT CTGTGTTTTA TAGAAAAATG ATTCCAGCAA TGGATAGAAG AGTCATTACA
    CCATACCAAG ATGGACCAGA TATTAGCTAT TTGGAAGTAG AAGATGGAGA TGTCTTTTTG
    AAAGAAGAAA TAAATATGGA ACAAAATTAT TCGGAAAGTA TGTTCAAGAA GGCAAAAACA
    AAAGCAAAAA AGAAGCCGCG TAAGCGTTCA GATAGTTCTG GAGGTTATAA CCTTTCAGAT
    GTTATTCAGA GTCCACCATC TACAGAACTG TTGACATCAG ACTCTGAAGG AAGCTATGCA
    GGAGTGGGTA GTCCTAGAGA TTTACAGTCC CCTGATTTCA CAGCAGGATT TCAGTCAGAT
    AAAATTGAGG TGAAAATTAA ACCATATGTT AATGGTACAC CTCCTGCATA TTCTAGGGAA
    GATTTAAAAC CATGGGAAAA ATCACCAATA CTTAACATAT CTGCTCCACA GACCATTCCT
    AGTAACAGAA TTGATACTAC AAACTCTTCT AGTTGGGTTG CTGGCTCTTT CAGTCCTGTC
    AGCCCTCCTG TTATGGATCT CAGAACCATC ATGCAAATTG AAGAAAGTAG ACAAAAATGT
    GGAGCTACAC CAAAGACAAA TTTGGGCAAG ATGATTTCTC ATGGAGTTAA ACTTTCTCAG
    AAGCAACGGA AAATGATTGC AATGACTACC AAAGAAAACA ATTCAGGAAC GAATAGCATG
    GAAACAGCTT TAACTGCTCC TTCAAAAACT CCAAAACCAG CAAACGCATG GGCATCTTCT
    GTACATTCAG TTTCATCCAA GTCATTCCGG GATTTCTTTG AAGAAAAAAA ATCTATTACT
    GGCCATAGTT CAGGAGATCA TGTCAAAAAA GTTTGTTTTA AAGGAATAGA AAATTCTCAG
    GCATCAAAAG TTGAAAGATG TTCCCCTCAT GGTACGCCAG GACCAGAAGG CAACCATATT
    TCAGATTTAC CACTTCTAGA CAGTCCCAAT CCCTGGCTGT CTTCTTCATT GACTGCTCCT
    CCTGTGGTAG CTCCAGTCAC TTTTGCATCT ATTGTAGAAG AAGAATTACA GCAGGAAGCA
    GCTTTAATCA GAAGTCGAGA AAAACCCTTG GCTTTGATTC AGATTGAAGA GCGTGCAATA
    CAAGATTTAT TGGTTTTCTA TGAGGCATTT GGCAACCCTG ATGAGTTTGT TATTGTTGAA
    AGGACACCAC AGGGACCACT AGCAGTACCT ATGTGGAATA AGCATGGATG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005627626.1
    CDS556..4569
    Translation

    Target ORF information:

    RefSeq Version XM_005627569.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005627569.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
    GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT
    TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC
    TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG
    AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT
    TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG
    TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT
    CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG
    AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG
    CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC
    TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT
    AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT
    ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA
    TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT
    CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT
    AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC
    AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC
    ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT
    ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA
    AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA
    AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG
    AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT
    TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT
    AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGCAGA GATTTTATCA
    AATCTTCATA ATTCCTCATC AGATGTGTCT TGTGTCTCTG ATATAAATAG TGTATACGAA
    AGAATTCGAC TTGAGAAACT TACTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGATCCA
    AGTGGATGCA ACTTTGCAAT CCTGCAGTCA GATCCTAAAA CAAGCCTTTA TGAAATTCCA
    GCCGTATCCT CATCATCCTT TTTTGAAGAG TTTGGCAAAC TCTTAAGGGA AACAGATGAA
    ACGGACAGTA TTCATGATGT GACATTTCAA GTTGGCAATA GAATTTTCCC TGCACATAAA
    TATATTTTGG CAGTGCGTTC TGACTTTTTT CAGAAATTGT TTCTTTCAGA TGGGACTACT
    TTAGATTTTA TAGATGTTTA CCGGAAAGAT GAAGATTCTG CAGGATGCCA TCTCTTTGTC
    ATAGAGAAGG TTCATCCTGA CTTGTTTGAG TACCTTTTAC AATTTATATA CACAGATACT
    TGTGACTTTT TAATTCATGG CTTCACACCA AGAATAAACT TAAACAAAAA GCCAGAAGAA
    TATCAGGGCA CTATGAATTC ACATTCAAAT AAAATGCATT GCCATGAAGA TAATCAGAAG
    TCAGCATTTG AAGTTTACAG AAGTAATCAA GCACACACAG TTAATGAAAA GCAAAAAAGC
    AAATCAAAGG CTTCCAATAA AGGGAAAAGT ATTGGGGAAG ATGATCCTGT AAGAATGTTG
    CAAAATGTTG CAAAGAAATT TGGTTTCAGT AATTTGAGTA GTAGGTTAGA TGGAGTCAGA
    TTTGAAAATG GAAAAATTAA TGTTATTGAG AAGAAAACTG GTAATAAATC AAAGCTAAAT
    CAGAAAAAAT GTTCCTTTCT ATGTGATGTG ACCATGAAAT CAGTGGATGG AAAGGAATTT
    CCTTGTCATA AGTGTGTTCT TTGTGCTAGA CTTGAATATT TTCATAGTAT GCTGAGTAGC
    TCATGGATTG AGGCTTCTAC TTGTACGGCT CTGGAAATGC CAATACATTC TGACATACTG
    GAAGTTATTT TGGACTACCT CTATACTGAT GAAGCTGTGG TGATAAAAGA ATCTCAAAAT
    GTGGATTTTG TTTGTAGTGT TCTCGTGGTG GCTGATCAGC TTCTTATAAC TCGATTGAAA
    GGGATTTGTG AAGTAGCATT AACTGAGAAA CTTACCCTTA AGAATGCTAC TATGGTACTG
    GAGTTTGCAG CAATGTATAA TGCTGAACAA TTGAAACTGT CTTGTTTGCA GTTTATAGGA
    CTGAATATGG CCGCTTTACT TGAAGCAAGG TCTCTTGATG TTTTAAGTGA TGGTGTTTTG
    AAGGATCTTT CTGTGTTTTA TAGAAAAATG ATTCCAGCAA TGGATAGAAG AGTCATTACA
    CCATACCAAG ATGGACCAGA TATTAGCTAT TTGGAAGTAG AAGATGGAGA TGTCTTTTTG
    AAAGAAGAAA TAAATATGGA ACAAAATTAT TCGGAAAGTA TGTTCAAGAA GGCAAAAACA
    AAAGCAAAAA AGAAGCCGCG TAAGCGTTCA GATAGTTCTG GAGGTTATAA CCTTTCAGAT
    GTTATTCAGA GTCCACCATC TACAGAACTG TTGACATCAG ACTCTGAAGG AAGCTATGCA
    GGAGTGGGTA GTCCTAGAGA TTTACAGTCC CCTGATTTCA CAGCAGGATT TCAGTCAGAT
    AAAATTGAGG TGAAAATTAA ACCATATGTT AATGGTACAC CTCCTGCATA TTCTAGGGAA
    GATTTAAAAC CATGGGAAAA ATCACCAATA CTTAACATAT CTGCTCCACA GACCATTCCT
    AGTAACAGAA TTGATACTAC AAACTCTTCT AGTTGGGTTG CTGGCTCTTT CAGTCCTGTC
    AGCCCTCCTG TTATGGATCT CAGAACCATC ATGCAAATTG AAGAAAGTAG ACAAAAATGT
    GGAGCTACAC CAAAGACAAA TTTGGGCAAG ATGATTTCTC ATGGAGTTAA ACTTTCTCAG
    AAGCAACGGA AAATGATTGC AATGACTACC AAAGAAAACA ATTCAGGAAC GAATAGCATG
    GAAACAGCTT TAACTGCTCC TTCAAAAACT CCAAAACCAG CAAACGCATG GGCATCTTCT
    GTACATTCAG TTTCATCCAA GTCATTCCGG GATTTCTTTG AAGAAAAAAA ATCTATTACT
    GGCCATAGTT CAGGAGATCA TGTCAAAAAA GTTTGTTTTA AAGGAATAGA AAATTCTCAG
    GCATCAAAAG TTGAAAGATG TTCCCCTCAT GGTACGCCAG GACCAGAAGG CAACCATATT
    TCAGATTTAC CACTTCTAGA CAGTCCCAAT CCCTGGCTGT CTTCTTCATT GACTGCTCCT
    CCTGTGGTAG CTCCAGTCAC TTTTGCATCT ATTGTAGAAG AAGAATTACA GCAGGAAGCA
    GCTTTAATCA GAAGTCGAGA AAAACCCTTG GCTTTGATTC AGATTGAAGA GCGTGCAATA
    CAAGATTTAT TGGTTTTCTA TGAGGCATTT GGCAACCCTG ATGAGTTTGT TATTGTTGAA
    AGGACACCAC AGGGACCACT AGCAGTACCT ATGTGGAATA AGCATGGATG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe15524
    Clone ID Related Accession (Same CDS sequence) XM_005627569.3 , XM_005627569.2
    Accession Version XM_005627569.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4014bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product inhibitor of Bruton tyrosine kinase isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005627569.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
    GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT
    TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC
    TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG
    AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT
    TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG
    TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT
    CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG
    AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG
    CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC
    TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT
    AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT
    ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA
    TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT
    CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT
    AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC
    AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC
    ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT
    ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA
    AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA
    AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG
    AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT
    TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT
    AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGCAGA GATTTTATCA
    AATCTTCATA ATTCCTCATC AGATGTGTCT TGTGTCTCTG ATATAAATAG TGTATACGAA
    AGAATTCGAC TTGAGAAACT TACTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGATCCA
    AGTGGATGCA ACTTTGCAAT CCTGCAGTCA GATCCTAAAA CAAGCCTTTA TGAAATTCCA
    GCCGTATCCT CATCATCCTT TTTTGAAGAG TTTGGCAAAC TCTTAAGGGA AACAGATGAA
    ACGGACAGTA TTCATGATGT GACATTTCAA GTTGGCAATA GAATTTTCCC TGCACATAAA
    TATATTTTGG CAGTGCGTTC TGACTTTTTT CAGAAATTGT TTCTTTCAGA TGGGACTACT
    TTAGATTTTA TAGATGTTTA CCGGAAAGAT GAAGATTCTG CAGGATGCCA TCTCTTTGTC
    ATAGAGAAGG TTCATCCTGA CTTGTTTGAG TACCTTTTAC AATTTATATA CACAGATACT
    TGTGACTTTT TAATTCATGG CTTCACACCA AGAATAAACT TAAACAAAAA GCCAGAAGAA
    TATCAGGGCA CTATGAATTC ACATTCAAAT AAAATGCATT GCCATGAAGA TAATCAGAAG
    TCAGCATTTG AAGTTTACAG AAGTAATCAA GCACACACAG TTAATGAAAA GCAAAAAAGC
    AAATCAAAGG CTTCCAATAA AGGGAAAAGT ATTGGGGAAG ATGATCCTGT AAGAATGTTG
    CAAAATGTTG CAAAGAAATT TGGTTTCAGT AATTTGAGTA GTAGGTTAGA TGGAGTCAGA
    TTTGAAAATG GAAAAATTAA TGTTATTGAG AAGAAAACTG GTAATAAATC AAAGCTAAAT
    CAGAAAAAAT GTTCCTTTCT ATGTGATGTG ACCATGAAAT CAGTGGATGG AAAGGAATTT
    CCTTGTCATA AGTGTGTTCT TTGTGCTAGA CTTGAATATT TTCATAGTAT GCTGAGTAGC
    TCATGGATTG AGGCTTCTAC TTGTACGGCT CTGGAAATGC CAATACATTC TGACATACTG
    GAAGTTATTT TGGACTACCT CTATACTGAT GAAGCTGTGG TGATAAAAGA ATCTCAAAAT
    GTGGATTTTG TTTGTAGTGT TCTCGTGGTG GCTGATCAGC TTCTTATAAC TCGATTGAAA
    GGGATTTGTG AAGTAGCATT AACTGAGAAA CTTACCCTTA AGAATGCTAC TATGGTACTG
    GAGTTTGCAG CAATGTATAA TGCTGAACAA TTGAAACTGT CTTGTTTGCA GTTTATAGGA
    CTGAATATGG CCGCTTTACT TGAAGCAAGG TCTCTTGATG TTTTAAGTGA TGGTGTTTTG
    AAGGATCTTT CTGTGTTTTA TAGAAAAATG ATTCCAGCAA TGGATAGAAG AGTCATTACA
    CCATACCAAG ATGGACCAGA TATTAGCTAT TTGGAAGTAG AAGATGGAGA TGTCTTTTTG
    AAAGAAGAAA TAAATATGGA ACAAAATTAT TCGGAAAGTA TGTTCAAGAA GGCAAAAACA
    AAAGCAAAAA AGAAGCCGCG TAAGCGTTCA GATAGTTCTG GAGGTTATAA CCTTTCAGAT
    GTTATTCAGA GTCCACCATC TACAGAACTG TTGACATCAG ACTCTGAAGG AAGCTATGCA
    GGAGTGGGTA GTCCTAGAGA TTTACAGTCC CCTGATTTCA CAGCAGGATT TCAGTCAGAT
    AAAATTGAGG TGAAAATTAA ACCATATGTT AATGGTACAC CTCCTGCATA TTCTAGGGAA
    GATTTAAAAC CATGGGAAAA ATCACCAATA CTTAACATAT CTGCTCCACA GACCATTCCT
    AGTAACAGAA TTGATACTAC AAACTCTTCT AGTTGGGTTG CTGGCTCTTT CAGTCCTGTC
    AGCCCTCCTG TTATGGATCT CAGAACCATC ATGCAAATTG AAGAAAGTAG ACAAAAATGT
    GGAGCTACAC CAAAGACAAA TTTGGGCAAG ATGATTTCTC ATGGAGTTAA ACTTTCTCAG
    AAGCAACGGA AAATGATTGC AATGACTACC AAAGAAAACA ATTCAGGAAC GAATAGCATG
    GAAACAGCTT TAACTGCTCC TTCAAAAACT CCAAAACCAG CAAACGCATG GGCATCTTCT
    GTACATTCAG TTTCATCCAA GTCATTCCGG GATTTCTTTG AAGAAAAAAA ATCTATTACT
    GGCCATAGTT CAGGAGATCA TGTCAAAAAA GTTTGTTTTA AAGGAATAGA AAATTCTCAG
    GCATCAAAAG TTGAAAGATG TTCCCCTCAT GGTACGCCAG GACCAGAAGG CAACCATATT
    TCAGATTTAC CACTTCTAGA CAGTCCCAAT CCCTGGCTGT CTTCTTCATT GACTGCTCCT
    CCTGTGGTAG CTCCAGTCAC TTTTGCATCT ATTGTAGAAG AAGAATTACA GCAGGAAGCA
    GCTTTAATCA GAAGTCGAGA AAAACCCTTG GCTTTGATTC AGATTGAAGA GCGTGCAATA
    CAAGATTTAT TGGTTTTCTA TGAGGCATTT GGCAACCCTG ATGAGTTTGT TATTGTTGAA
    AGGACACCAC AGGGACCACT AGCAGTACCT ATGTGGAATA AGCATGGATG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005627626.1
    CDS1231..5244
    Translation

    Target ORF information:

    RefSeq Version XM_005627569.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris inhibitor of Bruton agammaglobulinemia tyrosine kinase (IBTK), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005627569.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
    GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT
    TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC
    TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG
    AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT
    TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG
    TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT
    CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG
    AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG
    CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC
    TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT
    AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT
    ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA
    TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT
    CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT
    AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC
    AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC
    ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT
    ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA
    AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA
    AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG
    AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT
    TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT
    AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGCAGA GATTTTATCA
    AATCTTCATA ATTCCTCATC AGATGTGTCT TGTGTCTCTG ATATAAATAG TGTATACGAA
    AGAATTCGAC TTGAGAAACT TACTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGATCCA
    AGTGGATGCA ACTTTGCAAT CCTGCAGTCA GATCCTAAAA CAAGCCTTTA TGAAATTCCA
    GCCGTATCCT CATCATCCTT TTTTGAAGAG TTTGGCAAAC TCTTAAGGGA AACAGATGAA
    ACGGACAGTA TTCATGATGT GACATTTCAA GTTGGCAATA GAATTTTCCC TGCACATAAA
    TATATTTTGG CAGTGCGTTC TGACTTTTTT CAGAAATTGT TTCTTTCAGA TGGGACTACT
    TTAGATTTTA TAGATGTTTA CCGGAAAGAT GAAGATTCTG CAGGATGCCA TCTCTTTGTC
    ATAGAGAAGG TTCATCCTGA CTTGTTTGAG TACCTTTTAC AATTTATATA CACAGATACT
    TGTGACTTTT TAATTCATGG CTTCACACCA AGAATAAACT TAAACAAAAA GCCAGAAGAA
    TATCAGGGCA CTATGAATTC ACATTCAAAT AAAATGCATT GCCATGAAGA TAATCAGAAG
    TCAGCATTTG AAGTTTACAG AAGTAATCAA GCACACACAG TTAATGAAAA GCAAAAAAGC
    AAATCAAAGG CTTCCAATAA AGGGAAAAGT ATTGGGGAAG ATGATCCTGT AAGAATGTTG
    CAAAATGTTG CAAAGAAATT TGGTTTCAGT AATTTGAGTA GTAGGTTAGA TGGAGTCAGA
    TTTGAAAATG GAAAAATTAA TGTTATTGAG AAGAAAACTG GTAATAAATC AAAGCTAAAT
    CAGAAAAAAT GTTCCTTTCT ATGTGATGTG ACCATGAAAT CAGTGGATGG AAAGGAATTT
    CCTTGTCATA AGTGTGTTCT TTGTGCTAGA CTTGAATATT TTCATAGTAT GCTGAGTAGC
    TCATGGATTG AGGCTTCTAC TTGTACGGCT CTGGAAATGC CAATACATTC TGACATACTG
    GAAGTTATTT TGGACTACCT CTATACTGAT GAAGCTGTGG TGATAAAAGA ATCTCAAAAT
    GTGGATTTTG TTTGTAGTGT TCTCGTGGTG GCTGATCAGC TTCTTATAAC TCGATTGAAA
    GGGATTTGTG AAGTAGCATT AACTGAGAAA CTTACCCTTA AGAATGCTAC TATGGTACTG
    GAGTTTGCAG CAATGTATAA TGCTGAACAA TTGAAACTGT CTTGTTTGCA GTTTATAGGA
    CTGAATATGG CCGCTTTACT TGAAGCAAGG TCTCTTGATG TTTTAAGTGA TGGTGTTTTG
    AAGGATCTTT CTGTGTTTTA TAGAAAAATG ATTCCAGCAA TGGATAGAAG AGTCATTACA
    CCATACCAAG ATGGACCAGA TATTAGCTAT TTGGAAGTAG AAGATGGAGA TGTCTTTTTG
    AAAGAAGAAA TAAATATGGA ACAAAATTAT TCGGAAAGTA TGTTCAAGAA GGCAAAAACA
    AAAGCAAAAA AGAAGCCGCG TAAGCGTTCA GATAGTTCTG GAGGTTATAA CCTTTCAGAT
    GTTATTCAGA GTCCACCATC TACAGAACTG TTGACATCAG ACTCTGAAGG AAGCTATGCA
    GGAGTGGGTA GTCCTAGAGA TTTACAGTCC CCTGATTTCA CAGCAGGATT TCAGTCAGAT
    AAAATTGAGG TGAAAATTAA ACCATATGTT AATGGTACAC CTCCTGCATA TTCTAGGGAA
    GATTTAAAAC CATGGGAAAA ATCACCAATA CTTAACATAT CTGCTCCACA GACCATTCCT
    AGTAACAGAA TTGATACTAC AAACTCTTCT AGTTGGGTTG CTGGCTCTTT CAGTCCTGTC
    AGCCCTCCTG TTATGGATCT CAGAACCATC ATGCAAATTG AAGAAAGTAG ACAAAAATGT
    GGAGCTACAC CAAAGACAAA TTTGGGCAAG ATGATTTCTC ATGGAGTTAA ACTTTCTCAG
    AAGCAACGGA AAATGATTGC AATGACTACC AAAGAAAACA ATTCAGGAAC GAATAGCATG
    GAAACAGCTT TAACTGCTCC TTCAAAAACT CCAAAACCAG CAAACGCATG GGCATCTTCT
    GTACATTCAG TTTCATCCAA GTCATTCCGG GATTTCTTTG AAGAAAAAAA ATCTATTACT
    GGCCATAGTT CAGGAGATCA TGTCAAAAAA GTTTGTTTTA AAGGAATAGA AAATTCTCAG
    GCATCAAAAG TTGAAAGATG TTCCCCTCAT GGTACGCCAG GACCAGAAGG CAACCATATT
    TCAGATTTAC CACTTCTAGA CAGTCCCAAT CCCTGGCTGT CTTCTTCATT GACTGCTCCT
    CCTGTGGTAG CTCCAGTCAC TTTTGCATCT ATTGTAGAAG AAGAATTACA GCAGGAAGCA
    GCTTTAATCA GAAGTCGAGA AAAACCCTTG GCTTTGATTC AGATTGAAGA GCGTGCAATA
    CAAGATTTAT TGGTTTTCTA TGAGGCATTT GGCAACCCTG ATGAGTTTGT TATTGTTGAA
    AGGACACCAC AGGGACCACT AGCAGTACCT ATGTGGAATA AGCATGGATG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe15525
    Clone ID Related Accession (Same CDS sequence) XM_005627570.2 , XM_005627570.3
    Accession Version XM_005627570.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4011bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product inhibitor of Bruton tyrosine kinase isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005627570.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
    GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT
    TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC
    TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG
    AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT
    TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG
    TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT
    CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG
    AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG
    CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC
    TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT
    AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT
    ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA
    TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT
    CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT
    AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC
    AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC
    ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT
    ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA
    AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA
    AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG
    AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT
    TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT
    AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGAGAT TTTATCAAAT
    CTTCATAATT CCTCATCAGA TGTGTCTTGT GTCTCTGATA TAAATAGTGT ATACGAAAGA
    ATTCGACTTG AGAAACTTAC TTTTGCCCAT AGAGCTGTTA GTGTCAGCAC AGATCCAAGT
    GGATGCAACT TTGCAATCCT GCAGTCAGAT CCTAAAACAA GCCTTTATGA AATTCCAGCC
    GTATCCTCAT CATCCTTTTT TGAAGAGTTT GGCAAACTCT TAAGGGAAAC AGATGAAACG
    GACAGTATTC ATGATGTGAC ATTTCAAGTT GGCAATAGAA TTTTCCCTGC ACATAAATAT
    ATTTTGGCAG TGCGTTCTGA CTTTTTTCAG AAATTGTTTC TTTCAGATGG GACTACTTTA
    GATTTTATAG ATGTTTACCG GAAAGATGAA GATTCTGCAG GATGCCATCT CTTTGTCATA
    GAGAAGGTTC ATCCTGACTT GTTTGAGTAC CTTTTACAAT TTATATACAC AGATACTTGT
    GACTTTTTAA TTCATGGCTT CACACCAAGA ATAAACTTAA ACAAAAAGCC AGAAGAATAT
    CAGGGCACTA TGAATTCACA TTCAAATAAA ATGCATTGCC ATGAAGATAA TCAGAAGTCA
    GCATTTGAAG TTTACAGAAG TAATCAAGCA CACACAGTTA ATGAAAAGCA AAAAAGCAAA
    TCAAAGGCTT CCAATAAAGG GAAAAGTATT GGGGAAGATG ATCCTGTAAG AATGTTGCAA
    AATGTTGCAA AGAAATTTGG TTTCAGTAAT TTGAGTAGTA GGTTAGATGG AGTCAGATTT
    GAAAATGGAA AAATTAATGT TATTGAGAAG AAAACTGGTA ATAAATCAAA GCTAAATCAG
    AAAAAATGTT CCTTTCTATG TGATGTGACC ATGAAATCAG TGGATGGAAA GGAATTTCCT
    TGTCATAAGT GTGTTCTTTG TGCTAGACTT GAATATTTTC ATAGTATGCT GAGTAGCTCA
    TGGATTGAGG CTTCTACTTG TACGGCTCTG GAAATGCCAA TACATTCTGA CATACTGGAA
    GTTATTTTGG ACTACCTCTA TACTGATGAA GCTGTGGTGA TAAAAGAATC TCAAAATGTG
    GATTTTGTTT GTAGTGTTCT CGTGGTGGCT GATCAGCTTC TTATAACTCG ATTGAAAGGG
    ATTTGTGAAG TAGCATTAAC TGAGAAACTT ACCCTTAAGA ATGCTACTAT GGTACTGGAG
    TTTGCAGCAA TGTATAATGC TGAACAATTG AAACTGTCTT GTTTGCAGTT TATAGGACTG
    AATATGGCCG CTTTACTTGA AGCAAGGTCT CTTGATGTTT TAAGTGATGG TGTTTTGAAG
    GATCTTTCTG TGTTTTATAG AAAAATGATT CCAGCAATGG ATAGAAGAGT CATTACACCA
    TACCAAGATG GACCAGATAT TAGCTATTTG GAAGTAGAAG ATGGAGATGT CTTTTTGAAA
    GAAGAAATAA ATATGGAACA AAATTATTCG GAAAGTATGT TCAAGAAGGC AAAAACAAAA
    GCAAAAAAGA AGCCGCGTAA GCGTTCAGAT AGTTCTGGAG GTTATAACCT TTCAGATGTT
    ATTCAGAGTC CACCATCTAC AGAACTGTTG ACATCAGACT CTGAAGGAAG CTATGCAGGA
    GTGGGTAGTC CTAGAGATTT ACAGTCCCCT GATTTCACAG CAGGATTTCA GTCAGATAAA
    ATTGAGGTGA AAATTAAACC ATATGTTAAT GGTACACCTC CTGCATATTC TAGGGAAGAT
    TTAAAACCAT GGGAAAAATC ACCAATACTT AACATATCTG CTCCACAGAC CATTCCTAGT
    AACAGAATTG ATACTACAAA CTCTTCTAGT TGGGTTGCTG GCTCTTTCAG TCCTGTCAGC
    CCTCCTGTTA TGGATCTCAG AACCATCATG CAAATTGAAG AAAGTAGACA AAAATGTGGA
    GCTACACCAA AGACAAATTT GGGCAAGATG ATTTCTCATG GAGTTAAACT TTCTCAGAAG
    CAACGGAAAA TGATTGCAAT GACTACCAAA GAAAACAATT CAGGAACGAA TAGCATGGAA
    ACAGCTTTAA CTGCTCCTTC AAAAACTCCA AAACCAGCAA ACGCATGGGC ATCTTCTGTA
    CATTCAGTTT CATCCAAGTC ATTCCGGGAT TTCTTTGAAG AAAAAAAATC TATTACTGGC
    CATAGTTCAG GAGATCATGT CAAAAAAGTT TGTTTTAAAG GAATAGAAAA TTCTCAGGCA
    TCAAAAGTTG AAAGATGTTC CCCTCATGGT ACGCCAGGAC CAGAAGGCAA CCATATTTCA
    GATTTACCAC TTCTAGACAG TCCCAATCCC TGGCTGTCTT CTTCATTGAC TGCTCCTCCT
    GTGGTAGCTC CAGTCACTTT TGCATCTATT GTAGAAGAAG AATTACAGCA GGAAGCAGCT
    TTAATCAGAA GTCGAGAAAA ACCCTTGGCT TTGATTCAGA TTGAAGAGCG TGCAATACAA
    GATTTATTGG TTTTCTATGA GGCATTTGGC AACCCTGATG AGTTTGTTAT TGTTGAAAGG
    ACACCACAGG GACCACTAGC AGTACCTATG TGGAATAAGC ATGGATGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005627627.1
    CDS1231..5241
    Translation

    Target ORF information:

    RefSeq Version XM_005627570.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris inhibitor of Bruton agammaglobulinemia tyrosine kinase (IBTK), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005627570.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
    GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT
    TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC
    TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG
    AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT
    TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG
    TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT
    CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG
    AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG
    CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC
    TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT
    AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT
    ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA
    TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT
    CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT
    AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC
    AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC
    ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT
    ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA
    AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA
    AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG
    AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT
    TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT
    AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGAGAT TTTATCAAAT
    CTTCATAATT CCTCATCAGA TGTGTCTTGT GTCTCTGATA TAAATAGTGT ATACGAAAGA
    ATTCGACTTG AGAAACTTAC TTTTGCCCAT AGAGCTGTTA GTGTCAGCAC AGATCCAAGT
    GGATGCAACT TTGCAATCCT GCAGTCAGAT CCTAAAACAA GCCTTTATGA AATTCCAGCC
    GTATCCTCAT CATCCTTTTT TGAAGAGTTT GGCAAACTCT TAAGGGAAAC AGATGAAACG
    GACAGTATTC ATGATGTGAC ATTTCAAGTT GGCAATAGAA TTTTCCCTGC ACATAAATAT
    ATTTTGGCAG TGCGTTCTGA CTTTTTTCAG AAATTGTTTC TTTCAGATGG GACTACTTTA
    GATTTTATAG ATGTTTACCG GAAAGATGAA GATTCTGCAG GATGCCATCT CTTTGTCATA
    GAGAAGGTTC ATCCTGACTT GTTTGAGTAC CTTTTACAAT TTATATACAC AGATACTTGT
    GACTTTTTAA TTCATGGCTT CACACCAAGA ATAAACTTAA ACAAAAAGCC AGAAGAATAT
    CAGGGCACTA TGAATTCACA TTCAAATAAA ATGCATTGCC ATGAAGATAA TCAGAAGTCA
    GCATTTGAAG TTTACAGAAG TAATCAAGCA CACACAGTTA ATGAAAAGCA AAAAAGCAAA
    TCAAAGGCTT CCAATAAAGG GAAAAGTATT GGGGAAGATG ATCCTGTAAG AATGTTGCAA
    AATGTTGCAA AGAAATTTGG TTTCAGTAAT TTGAGTAGTA GGTTAGATGG AGTCAGATTT
    GAAAATGGAA AAATTAATGT TATTGAGAAG AAAACTGGTA ATAAATCAAA GCTAAATCAG
    AAAAAATGTT CCTTTCTATG TGATGTGACC ATGAAATCAG TGGATGGAAA GGAATTTCCT
    TGTCATAAGT GTGTTCTTTG TGCTAGACTT GAATATTTTC ATAGTATGCT GAGTAGCTCA
    TGGATTGAGG CTTCTACTTG TACGGCTCTG GAAATGCCAA TACATTCTGA CATACTGGAA
    GTTATTTTGG ACTACCTCTA TACTGATGAA GCTGTGGTGA TAAAAGAATC TCAAAATGTG
    GATTTTGTTT GTAGTGTTCT CGTGGTGGCT GATCAGCTTC TTATAACTCG ATTGAAAGGG
    ATTTGTGAAG TAGCATTAAC TGAGAAACTT ACCCTTAAGA ATGCTACTAT GGTACTGGAG
    TTTGCAGCAA TGTATAATGC TGAACAATTG AAACTGTCTT GTTTGCAGTT TATAGGACTG
    AATATGGCCG CTTTACTTGA AGCAAGGTCT CTTGATGTTT TAAGTGATGG TGTTTTGAAG
    GATCTTTCTG TGTTTTATAG AAAAATGATT CCAGCAATGG ATAGAAGAGT CATTACACCA
    TACCAAGATG GACCAGATAT TAGCTATTTG GAAGTAGAAG ATGGAGATGT CTTTTTGAAA
    GAAGAAATAA ATATGGAACA AAATTATTCG GAAAGTATGT TCAAGAAGGC AAAAACAAAA
    GCAAAAAAGA AGCCGCGTAA GCGTTCAGAT AGTTCTGGAG GTTATAACCT TTCAGATGTT
    ATTCAGAGTC CACCATCTAC AGAACTGTTG ACATCAGACT CTGAAGGAAG CTATGCAGGA
    GTGGGTAGTC CTAGAGATTT ACAGTCCCCT GATTTCACAG CAGGATTTCA GTCAGATAAA
    ATTGAGGTGA AAATTAAACC ATATGTTAAT GGTACACCTC CTGCATATTC TAGGGAAGAT
    TTAAAACCAT GGGAAAAATC ACCAATACTT AACATATCTG CTCCACAGAC CATTCCTAGT
    AACAGAATTG ATACTACAAA CTCTTCTAGT TGGGTTGCTG GCTCTTTCAG TCCTGTCAGC
    CCTCCTGTTA TGGATCTCAG AACCATCATG CAAATTGAAG AAAGTAGACA AAAATGTGGA
    GCTACACCAA AGACAAATTT GGGCAAGATG ATTTCTCATG GAGTTAAACT TTCTCAGAAG
    CAACGGAAAA TGATTGCAAT GACTACCAAA GAAAACAATT CAGGAACGAA TAGCATGGAA
    ACAGCTTTAA CTGCTCCTTC AAAAACTCCA AAACCAGCAA ACGCATGGGC ATCTTCTGTA
    CATTCAGTTT CATCCAAGTC ATTCCGGGAT TTCTTTGAAG AAAAAAAATC TATTACTGGC
    CATAGTTCAG GAGATCATGT CAAAAAAGTT TGTTTTAAAG GAATAGAAAA TTCTCAGGCA
    TCAAAAGTTG AAAGATGTTC CCCTCATGGT ACGCCAGGAC CAGAAGGCAA CCATATTTCA
    GATTTACCAC TTCTAGACAG TCCCAATCCC TGGCTGTCTT CTTCATTGAC TGCTCCTCCT
    GTGGTAGCTC CAGTCACTTT TGCATCTATT GTAGAAGAAG AATTACAGCA GGAAGCAGCT
    TTAATCAGAA GTCGAGAAAA ACCCTTGGCT TTGATTCAGA TTGAAGAGCG TGCAATACAA
    GATTTATTGG TTTTCTATGA GGCATTTGGC AACCCTGATG AGTTTGTTAT TGTTGAAAGG
    ACACCACAGG GACCACTAGC AGTACCTATG TGGAATAAGC ATGGATGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe15525
    Clone ID Related Accession (Same CDS sequence) XM_005627570.2 , XM_005627570.3
    Accession Version XM_005627570.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4011bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product inhibitor of Bruton tyrosine kinase isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005627570.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
    GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT
    TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC
    TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG
    AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT
    TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG
    TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT
    CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG
    AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG
    CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC
    TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT
    AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT
    ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA
    TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT
    CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT
    AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC
    AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC
    ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT
    ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA
    AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA
    AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG
    AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT
    TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT
    AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGAGAT TTTATCAAAT
    CTTCATAATT CCTCATCAGA TGTGTCTTGT GTCTCTGATA TAAATAGTGT ATACGAAAGA
    ATTCGACTTG AGAAACTTAC TTTTGCCCAT AGAGCTGTTA GTGTCAGCAC AGATCCAAGT
    GGATGCAACT TTGCAATCCT GCAGTCAGAT CCTAAAACAA GCCTTTATGA AATTCCAGCC
    GTATCCTCAT CATCCTTTTT TGAAGAGTTT GGCAAACTCT TAAGGGAAAC AGATGAAACG
    GACAGTATTC ATGATGTGAC ATTTCAAGTT GGCAATAGAA TTTTCCCTGC ACATAAATAT
    ATTTTGGCAG TGCGTTCTGA CTTTTTTCAG AAATTGTTTC TTTCAGATGG GACTACTTTA
    GATTTTATAG ATGTTTACCG GAAAGATGAA GATTCTGCAG GATGCCATCT CTTTGTCATA
    GAGAAGGTTC ATCCTGACTT GTTTGAGTAC CTTTTACAAT TTATATACAC AGATACTTGT
    GACTTTTTAA TTCATGGCTT CACACCAAGA ATAAACTTAA ACAAAAAGCC AGAAGAATAT
    CAGGGCACTA TGAATTCACA TTCAAATAAA ATGCATTGCC ATGAAGATAA TCAGAAGTCA
    GCATTTGAAG TTTACAGAAG TAATCAAGCA CACACAGTTA ATGAAAAGCA AAAAAGCAAA
    TCAAAGGCTT CCAATAAAGG GAAAAGTATT GGGGAAGATG ATCCTGTAAG AATGTTGCAA
    AATGTTGCAA AGAAATTTGG TTTCAGTAAT TTGAGTAGTA GGTTAGATGG AGTCAGATTT
    GAAAATGGAA AAATTAATGT TATTGAGAAG AAAACTGGTA ATAAATCAAA GCTAAATCAG
    AAAAAATGTT CCTTTCTATG TGATGTGACC ATGAAATCAG TGGATGGAAA GGAATTTCCT
    TGTCATAAGT GTGTTCTTTG TGCTAGACTT GAATATTTTC ATAGTATGCT GAGTAGCTCA
    TGGATTGAGG CTTCTACTTG TACGGCTCTG GAAATGCCAA TACATTCTGA CATACTGGAA
    GTTATTTTGG ACTACCTCTA TACTGATGAA GCTGTGGTGA TAAAAGAATC TCAAAATGTG
    GATTTTGTTT GTAGTGTTCT CGTGGTGGCT GATCAGCTTC TTATAACTCG ATTGAAAGGG
    ATTTGTGAAG TAGCATTAAC TGAGAAACTT ACCCTTAAGA ATGCTACTAT GGTACTGGAG
    TTTGCAGCAA TGTATAATGC TGAACAATTG AAACTGTCTT GTTTGCAGTT TATAGGACTG
    AATATGGCCG CTTTACTTGA AGCAAGGTCT CTTGATGTTT TAAGTGATGG TGTTTTGAAG
    GATCTTTCTG TGTTTTATAG AAAAATGATT CCAGCAATGG ATAGAAGAGT CATTACACCA
    TACCAAGATG GACCAGATAT TAGCTATTTG GAAGTAGAAG ATGGAGATGT CTTTTTGAAA
    GAAGAAATAA ATATGGAACA AAATTATTCG GAAAGTATGT TCAAGAAGGC AAAAACAAAA
    GCAAAAAAGA AGCCGCGTAA GCGTTCAGAT AGTTCTGGAG GTTATAACCT TTCAGATGTT
    ATTCAGAGTC CACCATCTAC AGAACTGTTG ACATCAGACT CTGAAGGAAG CTATGCAGGA
    GTGGGTAGTC CTAGAGATTT ACAGTCCCCT GATTTCACAG CAGGATTTCA GTCAGATAAA
    ATTGAGGTGA AAATTAAACC ATATGTTAAT GGTACACCTC CTGCATATTC TAGGGAAGAT
    TTAAAACCAT GGGAAAAATC ACCAATACTT AACATATCTG CTCCACAGAC CATTCCTAGT
    AACAGAATTG ATACTACAAA CTCTTCTAGT TGGGTTGCTG GCTCTTTCAG TCCTGTCAGC
    CCTCCTGTTA TGGATCTCAG AACCATCATG CAAATTGAAG AAAGTAGACA AAAATGTGGA
    GCTACACCAA AGACAAATTT GGGCAAGATG ATTTCTCATG GAGTTAAACT TTCTCAGAAG
    CAACGGAAAA TGATTGCAAT GACTACCAAA GAAAACAATT CAGGAACGAA TAGCATGGAA
    ACAGCTTTAA CTGCTCCTTC AAAAACTCCA AAACCAGCAA ACGCATGGGC ATCTTCTGTA
    CATTCAGTTT CATCCAAGTC ATTCCGGGAT TTCTTTGAAG AAAAAAAATC TATTACTGGC
    CATAGTTCAG GAGATCATGT CAAAAAAGTT TGTTTTAAAG GAATAGAAAA TTCTCAGGCA
    TCAAAAGTTG AAAGATGTTC CCCTCATGGT ACGCCAGGAC CAGAAGGCAA CCATATTTCA
    GATTTACCAC TTCTAGACAG TCCCAATCCC TGGCTGTCTT CTTCATTGAC TGCTCCTCCT
    GTGGTAGCTC CAGTCACTTT TGCATCTATT GTAGAAGAAG AATTACAGCA GGAAGCAGCT
    TTAATCAGAA GTCGAGAAAA ACCCTTGGCT TTGATTCAGA TTGAAGAGCG TGCAATACAA
    GATTTATTGG TTTTCTATGA GGCATTTGGC AACCCTGATG AGTTTGTTAT TGTTGAAAGG
    ACACCACAGG GACCACTAGC AGTACCTATG TGGAATAAGC ATGGATGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005627627.1
    CDS556..4566
    Translation

    Target ORF information:

    RefSeq Version XM_005627570.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005627570.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
    GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT
    TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC
    TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG
    AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT
    TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG
    TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT
    CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG
    AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG
    CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC
    TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT
    AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT
    ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA
    TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT
    CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT
    AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC
    AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC
    ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT
    ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA
    AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA
    AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG
    AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT
    TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT
    AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGAGAT TTTATCAAAT
    CTTCATAATT CCTCATCAGA TGTGTCTTGT GTCTCTGATA TAAATAGTGT ATACGAAAGA
    ATTCGACTTG AGAAACTTAC TTTTGCCCAT AGAGCTGTTA GTGTCAGCAC AGATCCAAGT
    GGATGCAACT TTGCAATCCT GCAGTCAGAT CCTAAAACAA GCCTTTATGA AATTCCAGCC
    GTATCCTCAT CATCCTTTTT TGAAGAGTTT GGCAAACTCT TAAGGGAAAC AGATGAAACG
    GACAGTATTC ATGATGTGAC ATTTCAAGTT GGCAATAGAA TTTTCCCTGC ACATAAATAT
    ATTTTGGCAG TGCGTTCTGA CTTTTTTCAG AAATTGTTTC TTTCAGATGG GACTACTTTA
    GATTTTATAG ATGTTTACCG GAAAGATGAA GATTCTGCAG GATGCCATCT CTTTGTCATA
    GAGAAGGTTC ATCCTGACTT GTTTGAGTAC CTTTTACAAT TTATATACAC AGATACTTGT
    GACTTTTTAA TTCATGGCTT CACACCAAGA ATAAACTTAA ACAAAAAGCC AGAAGAATAT
    CAGGGCACTA TGAATTCACA TTCAAATAAA ATGCATTGCC ATGAAGATAA TCAGAAGTCA
    GCATTTGAAG TTTACAGAAG TAATCAAGCA CACACAGTTA ATGAAAAGCA AAAAAGCAAA
    TCAAAGGCTT CCAATAAAGG GAAAAGTATT GGGGAAGATG ATCCTGTAAG AATGTTGCAA
    AATGTTGCAA AGAAATTTGG TTTCAGTAAT TTGAGTAGTA GGTTAGATGG AGTCAGATTT
    GAAAATGGAA AAATTAATGT TATTGAGAAG AAAACTGGTA ATAAATCAAA GCTAAATCAG
    AAAAAATGTT CCTTTCTATG TGATGTGACC ATGAAATCAG TGGATGGAAA GGAATTTCCT
    TGTCATAAGT GTGTTCTTTG TGCTAGACTT GAATATTTTC ATAGTATGCT GAGTAGCTCA
    TGGATTGAGG CTTCTACTTG TACGGCTCTG GAAATGCCAA TACATTCTGA CATACTGGAA
    GTTATTTTGG ACTACCTCTA TACTGATGAA GCTGTGGTGA TAAAAGAATC TCAAAATGTG
    GATTTTGTTT GTAGTGTTCT CGTGGTGGCT GATCAGCTTC TTATAACTCG ATTGAAAGGG
    ATTTGTGAAG TAGCATTAAC TGAGAAACTT ACCCTTAAGA ATGCTACTAT GGTACTGGAG
    TTTGCAGCAA TGTATAATGC TGAACAATTG AAACTGTCTT GTTTGCAGTT TATAGGACTG
    AATATGGCCG CTTTACTTGA AGCAAGGTCT CTTGATGTTT TAAGTGATGG TGTTTTGAAG
    GATCTTTCTG TGTTTTATAG AAAAATGATT CCAGCAATGG ATAGAAGAGT CATTACACCA
    TACCAAGATG GACCAGATAT TAGCTATTTG GAAGTAGAAG ATGGAGATGT CTTTTTGAAA
    GAAGAAATAA ATATGGAACA AAATTATTCG GAAAGTATGT TCAAGAAGGC AAAAACAAAA
    GCAAAAAAGA AGCCGCGTAA GCGTTCAGAT AGTTCTGGAG GTTATAACCT TTCAGATGTT
    ATTCAGAGTC CACCATCTAC AGAACTGTTG ACATCAGACT CTGAAGGAAG CTATGCAGGA
    GTGGGTAGTC CTAGAGATTT ACAGTCCCCT GATTTCACAG CAGGATTTCA GTCAGATAAA
    ATTGAGGTGA AAATTAAACC ATATGTTAAT GGTACACCTC CTGCATATTC TAGGGAAGAT
    TTAAAACCAT GGGAAAAATC ACCAATACTT AACATATCTG CTCCACAGAC CATTCCTAGT
    AACAGAATTG ATACTACAAA CTCTTCTAGT TGGGTTGCTG GCTCTTTCAG TCCTGTCAGC
    CCTCCTGTTA TGGATCTCAG AACCATCATG CAAATTGAAG AAAGTAGACA AAAATGTGGA
    GCTACACCAA AGACAAATTT GGGCAAGATG ATTTCTCATG GAGTTAAACT TTCTCAGAAG
    CAACGGAAAA TGATTGCAAT GACTACCAAA GAAAACAATT CAGGAACGAA TAGCATGGAA
    ACAGCTTTAA CTGCTCCTTC AAAAACTCCA AAACCAGCAA ACGCATGGGC ATCTTCTGTA
    CATTCAGTTT CATCCAAGTC ATTCCGGGAT TTCTTTGAAG AAAAAAAATC TATTACTGGC
    CATAGTTCAG GAGATCATGT CAAAAAAGTT TGTTTTAAAG GAATAGAAAA TTCTCAGGCA
    TCAAAAGTTG AAAGATGTTC CCCTCATGGT ACGCCAGGAC CAGAAGGCAA CCATATTTCA
    GATTTACCAC TTCTAGACAG TCCCAATCCC TGGCTGTCTT CTTCATTGAC TGCTCCTCCT
    GTGGTAGCTC CAGTCACTTT TGCATCTATT GTAGAAGAAG AATTACAGCA GGAAGCAGCT
    TTAATCAGAA GTCGAGAAAA ACCCTTGGCT TTGATTCAGA TTGAAGAGCG TGCAATACAA
    GATTTATTGG TTTTCTATGA GGCATTTGGC AACCCTGATG AGTTTGTTAT TGTTGAAAGG
    ACACCACAGG GACCACTAGC AGTACCTATG TGGAATAAGC ATGGATGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.