AKNA cDNA ORF clone, Canis lupus familiaris(Dog)

The following AKNA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AKNA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe14796 XM_014117950.2
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Canis lupus familiaris AT-hook transcription factor (AKNA), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
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OCe14796 XM_014117950.1 Canis lupus familiaris AT-hook transcription factor (AKNA), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe14796
    Clone ID Related Accession (Same CDS sequence) XM_014117950.2 , XM_014117950.1
    Accession Version XM_014117950.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4305bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product LOW QUALITY PROTEIN: AT-hook-containing transcription factor
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014117950.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 2 indels ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCCAGCT CCCGGACGGA GGTGCGCTGG GCCCAGCTGG GCCTGGGGAG GGGGCCCCAT 
    CGGCGACGCT GGGCCTGGCC CGAAGAGGAA AAGGATGGCG ACGGGAGCGG GGGAGAAGAG
    GGGCCCTTTC CCGGGGCCAC CAGCCCCGAG CTCCTGGACG ACTTCCGGCT GGCGCAGCAG
    CGCCTGCAGC CGCTGGAGTG GGACCCGGGC CCGCAGCCTG CAGAACGCCA GGATTCCGCA
    TCGGGAGAGT CTGCGGGCGA GGAGTTTGAA GCGGAGGATG TGGACAGCCC AGAAAGTTCC
    ATCGTGCCTC TCAGCTGGCT CCCTCAGCAG GATCCTCAAC TGGCCGTGAC TGAAGAGGAA
    CCGGATGAGG CCCCCGAAGG TCCGGAGGTG GAGGAAGCTG GAGAGAGCTC CCCAAGACTG
    GAGCATGAGA CTGGTCTCAG CTCAGGGGGC AATGGAAACA CCAGCCCCGG GGCTCTGGGG
    TGGGGTCAGG ACACAGGCCG TGTGGCCGCT GGCAAGCAAG CCGGTGGAGA CAAACTTCCA
    GAACATTCTG AGGTCAACCC AGCTATTGAC CTCAGCTCTG CAAGGTCCTG GAGCAGTGGG
    ACCGTGAGCC TTGACCAGCC CAATGACAGC CTCGAATCTA CCTGGGAAGG GGAAACTGAT
    GCCCCCCAGC CCGCCGCCCC GGCAGAAACT TTACCACGGA GCTCCAGCCA CCACCGGCTA
    CACGCAAACC CCAGAACCGG AGGCAGTGTT GCTTTGGCAA CGCCCACGGA ATTCCAGGAC
    TCCTCGGTAC CCCAGGCCCA GAGCCTCCAG CGTCCGGCCG GTAAGTGGAG AGAAAGTACC
    AGCCCTCACC TGGAGGACCA TGCTTGGAAA CGGACACGGA CGTCACCCAA GCCACTCCCT
    TCTCGGTTCG CCGGCTCCGT CAGCCCCCTG AATCCANCCA GGCCAGCCCG GCAAGACAGG
    CTGCCGCCCC GGCAGGGGGC CACTCTGGCT GGCCACTCGG CTGCCGATGC CTCCAAGTAC
    GGGCGGGGGC AGCTGAACTA CCCACTCGGT GATTTCTCCN AGGTGGGACC GCGGGTGAGG
    TTCCCCAAAG ATGAGAGCTA CCACCCCCCC AAGGCCAAGA GCCACGGCAG GCAGCCTCAG
    GGCCCTGCCA GGCCTCTGAT CTTCAAGTCA CCTGCTGAGA TTGTGCGGGA GGTACTGCTG
    AGCAACGGCG AAGCCTGCCC GGCCAAGGAC CCACCTCCTG CCCACCCCAT CACCAGGGTG
    CCCCAAGAAT TTCAGACACC CGAACAAGCC ACCGAGCTGG TCCATCAGCT CCAGGAGGAC
    TACCACAAGC TCCTCACCAA GTACGCCGAG GCCGAGAACA CCATCGACCA GCTGCGCCTT
    GGTGCCAAGG TGAACCTGTA TTCGGACCCG CTTCAGCCCA GCCACAGCAT CCACACAGGG
    ACGATGCCCC AGGGGACCAA GGTCTTATCC TTTACCATCC CACAGCCCCA GTCGGTGGTA
    TGGTGGTCAG GCTCCACCGA AGCCTCACAG ATCTCTGGGG CCTCAGGGTG GCCGTCCGCT
    CAAGGAGACC TGAGCCCCTG CTCGTCTGCC CCAGCGTGGC CCCCGGAGAA CCCAGGCAGC
    ACCCAGGACC AGCCCTCAGC AGCGCGGACC CAGGAATTGG CTTCTTGGGC CAACCAGTTC
    CTGGCCAAGG TGGAGTCCTT CGCAGGACTG CTGCAGGCAG GACGGCTCAC GCCCCAGGAC
    CAACTCAAGG GCTTCCAGCG CCTGAAGGCT GCCCACGCAG CCCTGGAGGA GGAGTACCTG
    AAGGCCTGCA GGGAGCAGCA GCTGGCCCAG CCGCCTCCCG GCTCCCAGGG GATGTCTGGA
    AAATTCGATC CTGGCAGGGA GCTAGAGGAG GAGATATTCC AGCTGGGAAT TCGCCTGGAA
    GAGCTGAAGG ACCGCATGGA GCAGAAGCAG CCAGAGCCTG AGCGAGCCGG GTCGGACTCG
    GCACCGGACA GCCCCCCGGC CACCACGTCC CCGCACCAGC CGGCCGACCT GCCCTCTCTC
    TCAGGCCCAG GCCGCACGCC CGCCGGCCAG CCCCGCCGCC CTCAGCCTGA CGCTGCCAGC
    TTAGGCCTCT GTGTGTCGCA CGGGAACGTG GAGCTTAGCG CCACCAGCAG TGAGGTGGAG
    GACAGGCCCC TGGGCCTCCC GGCCCCACTC AGGCACAAGG AGCTGCAGGT GGAGCAGGAT
    TTCCATGGCC TCCTGGAGCG GTATCTCAGC GTGAAGTCTC TCCCAGAAGC CATGAGGATG
    GAGGAGGAGG AGGGGGATGA GGCGGAGGAG GAGGAAGACG GCCAAGGCCG TATCCTGGAA
    GTGGATGGGC CAGCTCCAGC TCCGGGAAAA GCCGAGGCTT CCAGGATCCC CATAAGGCAG
    CTCCCAGCCC TGGCTGAAAG AAGTCACAGG GCCCCCCCCA AGGAGACGGT GGAGCAGTTG
    GGGTCTGTAA AGCCAGCAGG CTTCCATGCA TCCGCGGCCA GAGATGGCTA CCTCCAGGGC
    CTGGACAAAG CCGAGGCAAC TCCTCCAGGC CCTACCCGGC CGCCCCGCCC CCGGGCCACC
    AGGACTGCAG CATCCCACCA GAGCAGTCTG ACCAGCTTGG AGGGAAGTGG CATCTCTGAG
    CACCCTCCGC AGAAGTCTCT GCACCAGGCT GGGGGGCCTC ACCTAGAGGA GCCCTGGATG
    GCGTCCCCCG AGACAGACAG CGGCTTTGTG GGCTCAGAAA CCAGCAGAGT GTCGCCCCTC
    ACCCAAACAC CAGAGCACCG GCTCTCCCAC ATCAGCACTG CAGGAACACT GGCCCAGCCT
    TTCACTTCAT CTGAGCCTCA GGACACAGCC TCCCACCCCC AGGACGCAGC CTCCCCGCCC
    CAGACCAGGG GTCCTCCAGT CCCCAGAAGA GCCACGGAGC CCAGCACACC CAGGAGCCGA
    GCCCAGAGGC GCCCCTCGGG CCGGGACAGC CCTCTGCAGC AGAGGGCACC CAGCTTCCGC
    CTGGAACGGG CGCTGGTGGC CGAGATGGAG GTCCCCGGCT CAGAGTTCGA GGGGCGGAAG
    CGGATTTCCA AGCGTCTCCC CTGCAGCACG ACAATCAGCC CCCCGCCCGC CCTGGACCCT
    GCCGCCGCCC CTCTGCCCCA TGGAGCAGCA GAGAGCACCC ACACCTTCCT CCTCACCAGG
    ACAGGGCGAG ACCAGGCCAT CCGAGAGCTG CAAGAGGAGG TGTCTCGGCT CCGTCTGAGG
    CTGGAAGACA GCCTGCACCA GCCGCCCCAG GGCAGCCCGA CGTCCCCAGC GGCCGCCTTC
    GACCGCCCCG CCCGGCCCCG CGCCCGGCCA CTGGACCCCC CAGCTGCCTG GGGCTCCCAC
    TATGGCAGTA AATCCACAGA GAGATTGTCT GGTGAGCCCG GGGGTGCGCA GCAAGCTGAC
    CCCGCAGGAA GGCGGCGAGC CAGGTCTTCC TCAGCGCCTC GGGAGATGCC CCAACTGTCC
    GTGAGTTCTG AATCTGAGCC AACCCCCCCG CAGCTGTTCC CTGAGAAGAG CCGGGCGTCC
    ACGGACAGTG CAGAGGCAGC TCGGGACGGA GTGAAAGGAG CAGGGTCGGG CGCCAGGCGG
    CCCGACAGGG TCACCTTCCG GGGCCAGTAC ACAGGCCAGG AGTACCGTGT TCTGACCCCC
    ACGTCTGCCC CAAGAGGCGG TGGCACGATC TCCTGTCCCC ACTGCCGGCC CCCCAGGACT
    CAGGACTCAG GTGGTACCGT CACCAGGGAC CCACGGGGAC CGTCTCCCAC TGACACTGTG
    CGATGTCCCC TGTGTGGTCG AGGCGGGTCC TCCCCAGAGA CGGATGGCCC AGACTCAGCC
    CCCTGTGGGC CAGAAAGGGC TGCCGCAAGG AGAAACGCAC GCAGAGCCAG CAGCCCCAGC
    CAGCGGAGCA AGCGCACGGG GTCCCCGGCC CGGCCACCCC CCGGACTGTG GTATCTGGCT
    GCAGCGCCCG CAGTGCCAGC CCCTCCCGCC TTCGCCTACG TCTCCTCGGT TCCCATCATG
    CCTTATCCGT CAGCCACTGT GTACTACGCG CCCCCGGGAC CTACCTCAGC TTCCTCGGCC
    CAGCCGGCCC CCGCCGAGTG GCCCCCCCCA GCCCCGCCCC GCCTGCCCAG GGCGCCCCGG
    CACTCCATCC AGCTGAACCT GGAGGACCTA GAGGAGCTCA ACAAGGCCCT GAACCAAGCC
    GTCCAGGCTG CGGAGAACAT CCGCTCCACC ACCAAGCAGA TGAGCCGCTC CCTGTCCGCC
    GACCTGCGCC AGGCCCGAAG CCTGCGGGGC TCCTGCCTCT TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973425.1
    CDS176..4480
    Translation

    Target ORF information:

    RefSeq Version XM_014117950.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris AT-hook transcription factor (AKNA), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014117950.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCCAGCT CCCGGACGGA GGTGCGCTGG GCCCAGCTGG GCCTGGGGAG GGGGCCCCAT 
    CGGCGACGCT GGGCCTGGCC CGAAGAGGAA AAGGATGGCG ACGGGAGCGG GGGAGAAGAG
    GGGCCCTTTC CCGGGGCCAC CAGCCCCGAG CTCCTGGACG ACTTCCGGCT GGCGCAGCAG
    CGCCTGCAGC CGCTGGAGTG GGACCCGGGC CCGCAGCCTG CAGAACGCCA GGATTCCGCA
    TCGGGAGAGT CTGCGGGCGA GGAGTTTGAA GCGGAGGATG TGGACAGCCC AGAAAGTTCC
    ATCGTGCCTC TCAGCTGGCT CCCTCAGCAG GATCCTCAAC TGGCCGTGAC TGAAGAGGAA
    CCGGATGAGG CCCCCGAAGG TCCGGAGGTG GAGGAAGCTG GAGAGAGCTC CCCAAGACTG
    GAGCATGAGA CTGGTCTCAG CTCAGGGGGC AATGGAAACA CCAGCCCCGG GGCTCTGGGG
    TGGGGTCAGG ACACAGGCCG TGTGGCCGCT GGCAAGCAAG CCGGTGGAGA CAAACTTCCA
    GAACATTCTG AGGTCAACCC AGCTATTGAC CTCAGCTCTG CAAGGTCCTG GAGCAGTGGG
    ACCGTGAGCC TTGACCAGCC CAATGACAGC CTCGAATCTA CCTGGGAAGG GGAAACTGAT
    GCCCCCCAGC CCGCCGCCCC GGCAGAAACT TTACCACGGA GCTCCAGCCA CCACCGGCTA
    CACGCAAACC CCAGAACCGG AGGCAGTGTT GCTTTGGCAA CGCCCACGGA ATTCCAGGAC
    TCCTCGGTAC CCCAGGCCCA GAGCCTCCAG CGTCCGGCCG GTAAGTGGAG AGAAAGTACC
    AGCCCTCACC TGGAGGACCA TGCTTGGAAA CGGACACGGA CGTCACCCAA GCCACTCCCT
    TCTCGGTTCG CCGGCTCCGT CAGCCCCCTG AATCCANCCA GGCCAGCCCG GCAAGACAGG
    CTGCCGCCCC GGCAGGGGGC CACTCTGGCT GGCCACTCGG CTGCCGATGC CTCCAAGTAC
    GGGCGGGGGC AGCTGAACTA CCCACTCGGT GATTTCTCCN AGGTGGGACC GCGGGTGAGG
    TTCCCCAAAG ATGAGAGCTA CCACCCCCCC AAGGCCAAGA GCCACGGCAG GCAGCCTCAG
    GGCCCTGCCA GGCCTCTGAT CTTCAAGTCA CCTGCTGAGA TTGTGCGGGA GGTACTGCTG
    AGCAACGGCG AAGCCTGCCC GGCCAAGGAC CCACCTCCTG CCCACCCCAT CACCAGGGTG
    CCCCAAGAAT TTCAGACACC CGAACAAGCC ACCGAGCTGG TCCATCAGCT CCAGGAGGAC
    TACCACAAGC TCCTCACCAA GTACGCCGAG GCCGAGAACA CCATCGACCA GCTGCGCCTT
    GGTGCCAAGG TGAACCTGTA TTCGGACCCG CTTCAGCCCA GCCACAGCAT CCACACAGGG
    ACGATGCCCC AGGGGACCAA GGTCTTATCC TTTACCATCC CACAGCCCCA GTCGGTGGTA
    TGGTGGTCAG GCTCCACCGA AGCCTCACAG ATCTCTGGGG CCTCAGGGTG GCCGTCCGCT
    CAAGGAGACC TGAGCCCCTG CTCGTCTGCC CCAGCGTGGC CCCCGGAGAA CCCAGGCAGC
    ACCCAGGACC AGCCCTCAGC AGCGCGGACC CAGGAATTGG CTTCTTGGGC CAACCAGTTC
    CTGGCCAAGG TGGAGTCCTT CGCAGGACTG CTGCAGGCAG GACGGCTCAC GCCCCAGGAC
    CAACTCAAGG GCTTCCAGCG CCTGAAGGCT GCCCACGCAG CCCTGGAGGA GGAGTACCTG
    AAGGCCTGCA GGGAGCAGCA GCTGGCCCAG CCGCCTCCCG GCTCCCAGGG GATGTCTGGA
    AAATTCGATC CTGGCAGGGA GCTAGAGGAG GAGATATTCC AGCTGGGAAT TCGCCTGGAA
    GAGCTGAAGG ACCGCATGGA GCAGAAGCAG CCAGAGCCTG AGCGAGCCGG GTCGGACTCG
    GCACCGGACA GCCCCCCGGC CACCACGTCC CCGCACCAGC CGGCCGACCT GCCCTCTCTC
    TCAGGCCCAG GCCGCACGCC CGCCGGCCAG CCCCGCCGCC CTCAGCCTGA CGCTGCCAGC
    TTAGGCCTCT GTGTGTCGCA CGGGAACGTG GAGCTTAGCG CCACCAGCAG TGAGGTGGAG
    GACAGGCCCC TGGGCCTCCC GGCCCCACTC AGGCACAAGG AGCTGCAGGT GGAGCAGGAT
    TTCCATGGCC TCCTGGAGCG GTATCTCAGC GTGAAGTCTC TCCCAGAAGC CATGAGGATG
    GAGGAGGAGG AGGGGGATGA GGCGGAGGAG GAGGAAGACG GCCAAGGCCG TATCCTGGAA
    GTGGATGGGC CAGCTCCAGC TCCGGGAAAA GCCGAGGCTT CCAGGATCCC CATAAGGCAG
    CTCCCAGCCC TGGCTGAAAG AAGTCACAGG GCCCCCCCCA AGGAGACGGT GGAGCAGTTG
    GGGTCTGTAA AGCCAGCAGG CTTCCATGCA TCCGCGGCCA GAGATGGCTA CCTCCAGGGC
    CTGGACAAAG CCGAGGCAAC TCCTCCAGGC CCTACCCGGC CGCCCCGCCC CCGGGCCACC
    AGGACTGCAG CATCCCACCA GAGCAGTCTG ACCAGCTTGG AGGGAAGTGG CATCTCTGAG
    CACCCTCCGC AGAAGTCTCT GCACCAGGCT GGGGGGCCTC ACCTAGAGGA GCCCTGGATG
    GCGTCCCCCG AGACAGACAG CGGCTTTGTG GGCTCAGAAA CCAGCAGAGT GTCGCCCCTC
    ACCCAAACAC CAGAGCACCG GCTCTCCCAC ATCAGCACTG CAGGAACACT GGCCCAGCCT
    TTCACTTCAT CTGAGCCTCA GGACACAGCC TCCCACCCCC AGGACGCAGC CTCCCCGCCC
    CAGACCAGGG GTCCTCCAGT CCCCAGAAGA GCCACGGAGC CCAGCACACC CAGGAGCCGA
    GCCCAGAGGC GCCCCTCGGG CCGGGACAGC CCTCTGCAGC AGAGGGCACC CAGCTTCCGC
    CTGGAACGGG CGCTGGTGGC CGAGATGGAG GTCCCCGGCT CAGAGTTCGA GGGGCGGAAG
    CGGATTTCCA AGCGTCTCCC CTGCAGCACG ACAATCAGCC CCCCGCCCGC CCTGGACCCT
    GCCGCCGCCC CTCTGCCCCA TGGAGCAGCA GAGAGCACCC ACACCTTCCT CCTCACCAGG
    ACAGGGCGAG ACCAGGCCAT CCGAGAGCTG CAAGAGGAGG TGTCTCGGCT CCGTCTGAGG
    CTGGAAGACA GCCTGCACCA GCCGCCCCAG GGCAGCCCGA CGTCCCCAGC GGCCGCCTTC
    GACCGCCCCG CCCGGCCCCG CGCCCGGCCA CTGGACCCCC CAGCTGCCTG GGGCTCCCAC
    TATGGCAGTA AATCCACAGA GAGATTGTCT GGTGAGCCCG GGGGTGCGCA GCAAGCTGAC
    CCCGCAGGAA GGCGGCGAGC CAGGTCTTCC TCAGCGCCTC GGGAGATGCC CCAACTGTCC
    GTGAGTTCTG AATCTGAGCC AACCCCCCCG CAGCTGTTCC CTGAGAAGAG CCGGGCGTCC
    ACGGACAGTG CAGAGGCAGC TCGGGACGGA GTGAAAGGAG CAGGGTCGGG CGCCAGGCGG
    CCCGACAGGG TCACCTTCCG GGGCCAGTAC ACAGGCCAGG AGTACCGTGT TCTGACCCCC
    ACGTCTGCCC CAAGAGGCGG TGGCACGATC TCCTGTCCCC ACTGCCGGCC CCCCAGGACT
    CAGGACTCAG GTGGTACCGT CACCAGGGAC CCACGGGGAC CGTCTCCCAC TGACACTGTG
    CGATGTCCCC TGTGTGGTCG AGGCGGGTCC TCCCCAGAGA CGGATGGCCC AGACTCAGCC
    CCCTGTGGGC CAGAAAGGGC TGCCGCAAGG AGAAACGCAC GCAGAGCCAG CAGCCCCAGC
    CAGCGGAGCA AGCGCACGGG GTCCCCGGCC CGGCCACCCC CCGGACTGTG GTATCTGGCT
    GCAGCGCCCG CAGTGCCAGC CCCTCCCGCC TTCGCCTACG TCTCCTCGGT TCCCATCATG
    CCTTATCCGT CAGCCACTGT GTACTACGCG CCCCCGGGAC CTACCTCAGC TTCCTCGGCC
    CAGCCGGCCC CCGCCGAGTG GCCCCCCCCA GCCCCGCCCC GCCTGCCCAG GGCGCCCCGG
    CACTCCATCC AGCTGAACCT GGAGGACCTA GAGGAGCTCA ACAAGGCCCT GAACCAAGCC
    GTCCAGGCTG CGGAGAACAT CCGCTCCACC ACCAAGCAGA TGAGCCGCTC CCTGTCCGCC
    GACCTGCGCC AGGCCCGAAG CCTGCGGGGC TCCTGCCTCT TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe14796
    Clone ID Related Accession (Same CDS sequence) XM_014117950.2 , XM_014117950.1
    Accession Version XM_014117950.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4305bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product LOW QUALITY PROTEIN: AT-hook-containing transcription factor
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 2 indels ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCCAGCT CCCGGACGGA GGTGCGCTGG GCCCAGCTGG GCCTGGGGAG GGGGCCCCAT 
    CGGCGACGCT GGGCCTGGCC CGAAGAGGAA AAGGATGGCG ACGGGAGCGG GGGAGAAGAG
    GGGCCCTTTC CCGGGGCCAC CAGCCCCGAG CTCCTGGACG ACTTCCGGCT GGCGCAGCAG
    CGCCTGCAGC CGCTGGAGTG GGACCCGGGC CCGCAGCCTG CAGAACGCCA GGATTCCGCA
    TCGGGAGAGT CTGCGGGCGA GGAGTTTGAA GCGGAGGATG TGGACAGCCC AGAAAGTTCC
    ATCGTGCCTC TCAGCTGGCT CCCTCAGCAG GATCCTCAAC TGGCCGTGAC TGAAGAGGAA
    CCGGATGAGG CCCCCGAAGG TCCGGAGGTG GAGGAAGCTG GAGAGAGCTC CCCAAGACTG
    GAGCATGAGA CTGGTCTCAG CTCAGGGGGC AATGGAAACA CCAGCCCCGG GGCTCTGGGG
    TGGGGTCAGG ACACAGGCCG TGTGGCCGCT GGCAAGCAAG CCGGTGGAGA CAAACTTCCA
    GAACATTCTG AGGTCAACCC AGCTATTGAC CTCAGCTCTG CAAGGTCCTG GAGCAGTGGG
    ACCGTGAGCC TTGACCAGCC CAATGACAGC CTCGAATCTA CCTGGGAAGG GGAAACTGAT
    GCCCCCCAGC CCGCCGCCCC GGCAGAAACT TTACCACGGA GCTCCAGCCA CCACCGGCTA
    CACGCAAACC CCAGAACCGG AGGCAGTGTT GCTTTGGCAA CGCCCACGGA ATTCCAGGAC
    TCCTCGGTAC CCCAGGCCCA GAGCCTCCAG CGTCCGGCCG GTAAGTGGAG AGAAAGTACC
    AGCCCTCACC TGGAGGACCA TGCTTGGAAA CGGACACGGA CGTCACCCAA GCCACTCCCT
    TCTCGGTTCG CCGGCTCCGT CAGCCCCCTG AATCCANCCA GGCCAGCCCG GCAAGACAGG
    CTGCCGCCCC GGCAGGGGGC CACTCTGGCT GGCCACTCGG CTGCCGATGC CTCCAAGTAC
    GGGCGGGGGC AGCTGAACTA CCCACTCGGT GATTTCTCCN AGGTGGGACC GCGGGTGAGG
    TTCCCCAAAG ATGAGAGCTA CCACCCCCCC AAGGCCAAGA GCCACGGCAG GCAGCCTCAG
    GGCCCTGCCA GGCCTCTGAT CTTCAAGTCA CCTGCTGAGA TTGTGCGGGA GGTACTGCTG
    AGCAACGGCG AAGCCTGCCC GGCCAAGGAC CCACCTCCTG CCCACCCCAT CACCAGGGTG
    CCCCAAGAAT TTCAGACACC CGAACAAGCC ACCGAGCTGG TCCATCAGCT CCAGGAGGAC
    TACCACAAGC TCCTCACCAA GTACGCCGAG GCCGAGAACA CCATCGACCA GCTGCGCCTT
    GGTGCCAAGG TGAACCTGTA TTCGGACCCG CTTCAGCCCA GCCACAGCAT CCACACAGGG
    ACGATGCCCC AGGGGACCAA GGTCTTATCC TTTACCATCC CACAGCCCCA GTCGGTGGTA
    TGGTGGTCAG GCTCCACCGA AGCCTCACAG ATCTCTGGGG CCTCAGGGTG GCCGTCCGCT
    CAAGGAGACC TGAGCCCCTG CTCGTCTGCC CCAGCGTGGC CCCCGGAGAA CCCAGGCAGC
    ACCCAGGACC AGCCCTCAGC AGCGCGGACC CAGGAATTGG CTTCTTGGGC CAACCAGTTC
    CTGGCCAAGG TGGAGTCCTT CGCAGGACTG CTGCAGGCAG GACGGCTCAC GCCCCAGGAC
    CAACTCAAGG GCTTCCAGCG CCTGAAGGCT GCCCACGCAG CCCTGGAGGA GGAGTACCTG
    AAGGCCTGCA GGGAGCAGCA GCTGGCCCAG CCGCCTCCCG GCTCCCAGGG GATGTCTGGA
    AAATTCGATC CTGGCAGGGA GCTAGAGGAG GAGATATTCC AGCTGGGAAT TCGCCTGGAA
    GAGCTGAAGG ACCGCATGGA GCAGAAGCAG CCAGAGCCTG AGCGAGCCGG GTCGGACTCG
    GCACCGGACA GCCCCCCGGC CACCACGTCC CCGCACCAGC CGGCCGACCT GCCCTCTCTC
    TCAGGCCCAG GCCGCACGCC CGCCGGCCAG CCCCGCCGCC CTCAGCCTGA CGCTGCCAGC
    TTAGGCCTCT GTGTGTCGCA CGGGAACGTG GAGCTTAGCG CCACCAGCAG TGAGGTGGAG
    GACAGGCCCC TGGGCCTCCC GGCCCCACTC AGGCACAAGG AGCTGCAGGT GGAGCAGGAT
    TTCCATGGCC TCCTGGAGCG GTATCTCAGC GTGAAGTCTC TCCCAGAAGC CATGAGGATG
    GAGGAGGAGG AGGGGGATGA GGCGGAGGAG GAGGAAGACG GCCAAGGCCG TATCCTGGAA
    GTGGATGGGC CAGCTCCAGC TCCGGGAAAA GCCGAGGCTT CCAGGATCCC CATAAGGCAG
    CTCCCAGCCC TGGCTGAAAG AAGTCACAGG GCCCCCCCCA AGGAGACGGT GGAGCAGTTG
    GGGTCTGTAA AGCCAGCAGG CTTCCATGCA TCCGCGGCCA GAGATGGCTA CCTCCAGGGC
    CTGGACAAAG CCGAGGCAAC TCCTCCAGGC CCTACCCGGC CGCCCCGCCC CCGGGCCACC
    AGGACTGCAG CATCCCACCA GAGCAGTCTG ACCAGCTTGG AGGGAAGTGG CATCTCTGAG
    CACCCTCCGC AGAAGTCTCT GCACCAGGCT GGGGGGCCTC ACCTAGAGGA GCCCTGGATG
    GCGTCCCCCG AGACAGACAG CGGCTTTGTG GGCTCAGAAA CCAGCAGAGT GTCGCCCCTC
    ACCCAAACAC CAGAGCACCG GCTCTCCCAC ATCAGCACTG CAGGAACACT GGCCCAGCCT
    TTCACTTCAT CTGAGCCTCA GGACACAGCC TCCCACCCCC AGGACGCAGC CTCCCCGCCC
    CAGACCAGGG GTCCTCCAGT CCCCAGAAGA GCCACGGAGC CCAGCACACC CAGGAGCCGA
    GCCCAGAGGC GCCCCTCGGG CCGGGACAGC CCTCTGCAGC AGAGGGCACC CAGCTTCCGC
    CTGGAACGGG CGCTGGTGGC CGAGATGGAG GTCCCCGGCT CAGAGTTCGA GGGGCGGAAG
    CGGATTTCCA AGCGTCTCCC CTGCAGCACG ACAATCAGCC CCCCGCCCGC CCTGGACCCT
    GCCGCCGCCC CTCTGCCCCA TGGAGCAGCA GAGAGCACCC ACACCTTCCT CCTCACCAGG
    ACAGGGCGAG ACCAGGCCAT CCGAGAGCTG CAAGAGGAGG TGTCTCGGCT CCGTCTGAGG
    CTGGAAGACA GCCTGCACCA GCCGCCCCAG GGCAGCCCGA CGTCCCCAGC GGCCGCCTTC
    GACCGCCCCG CCCGGCCCCG CGCCCGGCCA CTGGACCCCC CAGCTGCCTG GGGCTCCCAC
    TATGGCAGTA AATCCACAGA GAGATTGTCT GGTGAGCCCG GGGGTGCGCA GCAAGCTGAC
    CCCGCAGGAA GGCGGCGAGC CAGGTCTTCC TCAGCGCCTC GGGAGATGCC CCAACTGTCC
    GTGAGTTCTG AATCTGAGCC AACCCCCCCG CAGCTGTTCC CTGAGAAGAG CCGGGCGTCC
    ACGGACAGTG CAGAGGCAGC TCGGGACGGA GTGAAAGGAG CAGGGTCGGG CGCCAGGCGG
    CCCGACAGGG TCACCTTCCG GGGCCAGTAC ACAGGCCAGG AGTACCGTGT TCTGACCCCC
    ACGTCTGCCC CAAGAGGCGG TGGCACGATC TCCTGTCCCC ACTGCCGGCC CCCCAGGACT
    CAGGACTCAG GTGGTACCGT CACCAGGGAC CCACGGGGAC CGTCTCCCAC TGACACTGTG
    CGATGTCCCC TGTGTGGTCG AGGCGGGTCC TCCCCAGAGA CGGATGGCCC AGACTCAGCC
    CCCTGTGGGC CAGAAAGGGC TGCCGCAAGG AGAAACGCAC GCAGAGCCAG CAGCCCCAGC
    CAGCGGAGCA AGCGCACGGG GTCCCCGGCC CGGCCACCCC CCGGACTGTG GTATCTGGCT
    GCAGCGCCCG CAGTGCCAGC CCCTCCCGCC TTCGCCTACG TCTCCTCGGT TCCCATCATG
    CCTTATCCGT CAGCCACTGT GTACTACGCG CCCCCGGGAC CTACCTCAGC TTCCTCGGCC
    CAGCCGGCCC CCGCCGAGTG GCCCCCCCCA GCCCCGCCCC GCCTGCCCAG GGCGCCCCGG
    CACTCCATCC AGCTGAACCT GGAGGACCTA GAGGAGCTCA ACAAGGCCCT GAACCAAGCC
    GTCCAGGCTG CGGAGAACAT CCGCTCCACC ACCAAGCAGA TGAGCCGCTC CCTGTCCGCC
    GACCTGCGCC AGGCCCGAAG CCTGCGGGGC TCCTGCCTCT TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973425.1
    CDS145..4449
    Translation

    Target ORF information:

    RefSeq Version XM_014117950.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris AT-hook transcription factor (AKNA), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014117950.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCCAGCT CCCGGACGGA GGTGCGCTGG GCCCAGCTGG GCCTGGGGAG GGGGCCCCAT 
    CGGCGACGCT GGGCCTGGCC CGAAGAGGAA AAGGATGGCG ACGGGAGCGG GGGAGAAGAG
    GGGCCCTTTC CCGGGGCCAC CAGCCCCGAG CTCCTGGACG ACTTCCGGCT GGCGCAGCAG
    CGCCTGCAGC CGCTGGAGTG GGACCCGGGC CCGCAGCCTG CAGAACGCCA GGATTCCGCA
    TCGGGAGAGT CTGCGGGCGA GGAGTTTGAA GCGGAGGATG TGGACAGCCC AGAAAGTTCC
    ATCGTGCCTC TCAGCTGGCT CCCTCAGCAG GATCCTCAAC TGGCCGTGAC TGAAGAGGAA
    CCGGATGAGG CCCCCGAAGG TCCGGAGGTG GAGGAAGCTG GAGAGAGCTC CCCAAGACTG
    GAGCATGAGA CTGGTCTCAG CTCAGGGGGC AATGGAAACA CCAGCCCCGG GGCTCTGGGG
    TGGGGTCAGG ACACAGGCCG TGTGGCCGCT GGCAAGCAAG CCGGTGGAGA CAAACTTCCA
    GAACATTCTG AGGTCAACCC AGCTATTGAC CTCAGCTCTG CAAGGTCCTG GAGCAGTGGG
    ACCGTGAGCC TTGACCAGCC CAATGACAGC CTCGAATCTA CCTGGGAAGG GGAAACTGAT
    GCCCCCCAGC CCGCCGCCCC GGCAGAAACT TTACCACGGA GCTCCAGCCA CCACCGGCTA
    CACGCAAACC CCAGAACCGG AGGCAGTGTT GCTTTGGCAA CGCCCACGGA ATTCCAGGAC
    TCCTCGGTAC CCCAGGCCCA GAGCCTCCAG CGTCCGGCCG GTAAGTGGAG AGAAAGTACC
    AGCCCTCACC TGGAGGACCA TGCTTGGAAA CGGACACGGA CGTCACCCAA GCCACTCCCT
    TCTCGGTTCG CCGGCTCCGT CAGCCCCCTG AATCCANCCA GGCCAGCCCG GCAAGACAGG
    CTGCCGCCCC GGCAGGGGGC CACTCTGGCT GGCCACTCGG CTGCCGATGC CTCCAAGTAC
    GGGCGGGGGC AGCTGAACTA CCCACTCGGT GATTTCTCCN AGGTGGGACC GCGGGTGAGG
    TTCCCCAAAG ATGAGAGCTA CCACCCCCCC AAGGCCAAGA GCCACGGCAG GCAGCCTCAG
    GGCCCTGCCA GGCCTCTGAT CTTCAAGTCA CCTGCTGAGA TTGTGCGGGA GGTACTGCTG
    AGCAACGGCG AAGCCTGCCC GGCCAAGGAC CCACCTCCTG CCCACCCCAT CACCAGGGTG
    CCCCAAGAAT TTCAGACACC CGAACAAGCC ACCGAGCTGG TCCATCAGCT CCAGGAGGAC
    TACCACAAGC TCCTCACCAA GTACGCCGAG GCCGAGAACA CCATCGACCA GCTGCGCCTT
    GGTGCCAAGG TGAACCTGTA TTCGGACCCG CTTCAGCCCA GCCACAGCAT CCACACAGGG
    ACGATGCCCC AGGGGACCAA GGTCTTATCC TTTACCATCC CACAGCCCCA GTCGGTGGTA
    TGGTGGTCAG GCTCCACCGA AGCCTCACAG ATCTCTGGGG CCTCAGGGTG GCCGTCCGCT
    CAAGGAGACC TGAGCCCCTG CTCGTCTGCC CCAGCGTGGC CCCCGGAGAA CCCAGGCAGC
    ACCCAGGACC AGCCCTCAGC AGCGCGGACC CAGGAATTGG CTTCTTGGGC CAACCAGTTC
    CTGGCCAAGG TGGAGTCCTT CGCAGGACTG CTGCAGGCAG GACGGCTCAC GCCCCAGGAC
    CAACTCAAGG GCTTCCAGCG CCTGAAGGCT GCCCACGCAG CCCTGGAGGA GGAGTACCTG
    AAGGCCTGCA GGGAGCAGCA GCTGGCCCAG CCGCCTCCCG GCTCCCAGGG GATGTCTGGA
    AAATTCGATC CTGGCAGGGA GCTAGAGGAG GAGATATTCC AGCTGGGAAT TCGCCTGGAA
    GAGCTGAAGG ACCGCATGGA GCAGAAGCAG CCAGAGCCTG AGCGAGCCGG GTCGGACTCG
    GCACCGGACA GCCCCCCGGC CACCACGTCC CCGCACCAGC CGGCCGACCT GCCCTCTCTC
    TCAGGCCCAG GCCGCACGCC CGCCGGCCAG CCCCGCCGCC CTCAGCCTGA CGCTGCCAGC
    TTAGGCCTCT GTGTGTCGCA CGGGAACGTG GAGCTTAGCG CCACCAGCAG TGAGGTGGAG
    GACAGGCCCC TGGGCCTCCC GGCCCCACTC AGGCACAAGG AGCTGCAGGT GGAGCAGGAT
    TTCCATGGCC TCCTGGAGCG GTATCTCAGC GTGAAGTCTC TCCCAGAAGC CATGAGGATG
    GAGGAGGAGG AGGGGGATGA GGCGGAGGAG GAGGAAGACG GCCAAGGCCG TATCCTGGAA
    GTGGATGGGC CAGCTCCAGC TCCGGGAAAA GCCGAGGCTT CCAGGATCCC CATAAGGCAG
    CTCCCAGCCC TGGCTGAAAG AAGTCACAGG GCCCCCCCCA AGGAGACGGT GGAGCAGTTG
    GGGTCTGTAA AGCCAGCAGG CTTCCATGCA TCCGCGGCCA GAGATGGCTA CCTCCAGGGC
    CTGGACAAAG CCGAGGCAAC TCCTCCAGGC CCTACCCGGC CGCCCCGCCC CCGGGCCACC
    AGGACTGCAG CATCCCACCA GAGCAGTCTG ACCAGCTTGG AGGGAAGTGG CATCTCTGAG
    CACCCTCCGC AGAAGTCTCT GCACCAGGCT GGGGGGCCTC ACCTAGAGGA GCCCTGGATG
    GCGTCCCCCG AGACAGACAG CGGCTTTGTG GGCTCAGAAA CCAGCAGAGT GTCGCCCCTC
    ACCCAAACAC CAGAGCACCG GCTCTCCCAC ATCAGCACTG CAGGAACACT GGCCCAGCCT
    TTCACTTCAT CTGAGCCTCA GGACACAGCC TCCCACCCCC AGGACGCAGC CTCCCCGCCC
    CAGACCAGGG GTCCTCCAGT CCCCAGAAGA GCCACGGAGC CCAGCACACC CAGGAGCCGA
    GCCCAGAGGC GCCCCTCGGG CCGGGACAGC CCTCTGCAGC AGAGGGCACC CAGCTTCCGC
    CTGGAACGGG CGCTGGTGGC CGAGATGGAG GTCCCCGGCT CAGAGTTCGA GGGGCGGAAG
    CGGATTTCCA AGCGTCTCCC CTGCAGCACG ACAATCAGCC CCCCGCCCGC CCTGGACCCT
    GCCGCCGCCC CTCTGCCCCA TGGAGCAGCA GAGAGCACCC ACACCTTCCT CCTCACCAGG
    ACAGGGCGAG ACCAGGCCAT CCGAGAGCTG CAAGAGGAGG TGTCTCGGCT CCGTCTGAGG
    CTGGAAGACA GCCTGCACCA GCCGCCCCAG GGCAGCCCGA CGTCCCCAGC GGCCGCCTTC
    GACCGCCCCG CCCGGCCCCG CGCCCGGCCA CTGGACCCCC CAGCTGCCTG GGGCTCCCAC
    TATGGCAGTA AATCCACAGA GAGATTGTCT GGTGAGCCCG GGGGTGCGCA GCAAGCTGAC
    CCCGCAGGAA GGCGGCGAGC CAGGTCTTCC TCAGCGCCTC GGGAGATGCC CCAACTGTCC
    GTGAGTTCTG AATCTGAGCC AACCCCCCCG CAGCTGTTCC CTGAGAAGAG CCGGGCGTCC
    ACGGACAGTG CAGAGGCAGC TCGGGACGGA GTGAAAGGAG CAGGGTCGGG CGCCAGGCGG
    CCCGACAGGG TCACCTTCCG GGGCCAGTAC ACAGGCCAGG AGTACCGTGT TCTGACCCCC
    ACGTCTGCCC CAAGAGGCGG TGGCACGATC TCCTGTCCCC ACTGCCGGCC CCCCAGGACT
    CAGGACTCAG GTGGTACCGT CACCAGGGAC CCACGGGGAC CGTCTCCCAC TGACACTGTG
    CGATGTCCCC TGTGTGGTCG AGGCGGGTCC TCCCCAGAGA CGGATGGCCC AGACTCAGCC
    CCCTGTGGGC CAGAAAGGGC TGCCGCAAGG AGAAACGCAC GCAGAGCCAG CAGCCCCAGC
    CAGCGGAGCA AGCGCACGGG GTCCCCGGCC CGGCCACCCC CCGGACTGTG GTATCTGGCT
    GCAGCGCCCG CAGTGCCAGC CCCTCCCGCC TTCGCCTACG TCTCCTCGGT TCCCATCATG
    CCTTATCCGT CAGCCACTGT GTACTACGCG CCCCCGGGAC CTACCTCAGC TTCCTCGGCC
    CAGCCGGCCC CCGCCGAGTG GCCCCCCCCA GCCCCGCCCC GCCTGCCCAG GGCGCCCCGG
    CACTCCATCC AGCTGAACCT GGAGGACCTA GAGGAGCTCA ACAAGGCCCT GAACCAAGCC
    GTCCAGGCTG CGGAGAACAT CCGCTCCACC ACCAAGCAGA TGAGCCGCTC CCTGTCCGCC
    GACCTGCGCC AGGCCCGAAG CCTGCGGGGC TCCTGCCTCT TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.