ROCK1 cDNA ORF clone, Canis lupus familiaris(dog)

The following ROCK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ROCK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe289826 XM_022421535.1
Latest version!
Canis lupus familiaris Rho associated coiled-coil containing protein kinase 1 (ROCK1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe289827 XM_022421536.1
Latest version!
Canis lupus familiaris Rho associated coiled-coil containing protein kinase 1 (ROCK1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe09897 XM_005623022.3
Latest version!
Canis lupus familiaris Rho associated coiled-coil containing protein kinase 1 (ROCK1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe09898 XM_005623023.3
Latest version!
Canis lupus familiaris Rho associated coiled-coil containing protein kinase 1 (ROCK1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OCe09897 XM_005623022.2 Canis lupus familiaris Rho-associated, coiled-coil containing protein kinase 1 (ROCK1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe09898 XM_005623023.2 Canis lupus familiaris Rho-associated, coiled-coil containing protein kinase 1 (ROCK1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe289826
    Clone ID Related Accession (Same CDS sequence) XM_022421535.1
    Accession Version XM_022421535.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4065bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rho-associated protein kinase 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCGACTG GGGACAGTTT TGAGGCTCGG TTTGAAAAAA TCGACAACCT GCTGCGGGAT 
    CCGAAATCAG AAGTGAATTC GGATTGTTTG CTGGATGGAT TGGATGCTTT GGTATATGAT
    TTGGATTTTC CTGCCTTAAG AAAAAACAAA AATATTGACA ACTTTTTAAG CAGATATAAA
    GACACAATAA ATAAAATTAG AGATTTACGA ATGAAAGCTG AAGATTATGA AGTAGTGAAG
    GTGATTGGTC GAGGTGCATT TGGAGAAGTT CAATTGGTAA GGCATAAATC CACCAGGAAG
    GTATATGCTA TGAAGCTTCT CAGTAAATTT GAAATGATTA AGAGATCTGA TTCTGCTTTT
    TTCTGGGAAG AAAGGGACAT CATGGCTTTT GCTAATAGCC CTTGGGTCGT CCAGCTTTTT
    TATGCATTCC AAGATGATCG TTATCTCTAC ATGGTGATGG AATACATGCC TGGTGGAGAT
    CTTGTAAATT TAATGAGCAA CTATGATGTG CCTGAAAAAT GGGCACGATT CTATACTGCA
    GAAGTAGTAC TTGCGTTGGA TGCAATCCAT TCCATGGGTT TCATTCATAG AGATGTGAAG
    CCTGATAACA TGCTGCTGGA TAAATCTGGA CATTTGAAGT TAGCAGATTT TGGTACTTGT
    ATGAAGATGA ATAAGGAAGG TATGGTACGA TGTGATACAG CAGTTGGAAC ACCTGATTAC
    ATTTCCCCTG AAGTATTAAA ATCTCAAGGT GGTGATGGCT ATTATGGACG AGAATGTGAC
    TGGTGGTCAG TTGGGGTATT TTTATATGAA ATGCTTGTAG GTGATACACC TTTTTATGCA
    GATTCTCTGG TAGGAACTTA CAGTAAGATT ATGAACCATA AAAATTCACT TACTTTCCCT
    GATGATAATG ATATATCAAA AGAAGCAAAA AATCTTATTT GTGCCTTCCT TACTGACAGA
    GAGGTGAGAT TAGGGCGAAA TGGTGTAGAA GAAATCAAAC GACATCTCTT CTTCAAAAAT
    GATCAATGGG CTTGGGAGAC ACTCCGAGAC ACTGTAGCAC CAGTTGTACC TGATTTAAGT
    AGTGACATTG ATACAAGTAA TTTTGATGAC TTGGAAGAAG ATAAAGGAGA TGAAGAAACG
    TTTCCCATAC CTAAAGCTTT TGTTGGCAAT CAGTTACCCT TTGTAGGATT TACATATTAT
    AGCAATCGTA GATACTTATC TTCAGCAAAT CCTAATGATA ACCGAAGTAG CCCCAATGTG
    GATAAAAGCT TGCAGGAAAA TTTGCAAAAA ACAATTTATA AGCTGGAAGA ACAGCTGCAT
    AATGAAATGC AATTAAAAGA TGAAATGGAG CAGAAATGCA GAACTTCAAA CATAAAACTA
    GACAAGATAA TGAAAGAATT GGATGAAGAG GGGAATCAAA GAAGAAACCT AGAATCTACA
    GTGTCTCAGA TTGAGAAGGA GAAAATGTTG CTACAGCATA GAATTAATGA GTACCAGAGA
    AAAGCAGAAC AGGAAAATGA GAAAAGAAGG AATGTAGAAC ATGAAGTTTC TACATTAAAG
    GATCAGTTGG AAGACTTAAA AAAAGTAAAT CAGAATTCAC AGCTTGCTAA TGAGAAACTT
    GCACAATTAC AAAAACAGCT AGAAGAAGCC AATGACTTAC TTAGGACAGA ATCAGACACA
    GCTGTAAGAT TAAGGAAGAG CCACACAGAA ATGAGTAAGT CAGTTAGTCA GCTAGAATCT
    CTGAACAGAG AATTGCAAGA GAGAAACAGA ATTTTAGAGA ATTCCAAGAC ACAGATGGAC
    AAGGACTATT ACCAGCTGCA GGCTGTATTA GAAGCTGAAC GAAGAGACAG AGGTCATGAT
    TCTGAGATGA TTGGAGACCT TCAAGCTCGA ATTACATCCT TACAAGAGGA GGTGAAGCAT
    CTCAAACATA ATCTTGAAAG AGTGGAGGGA GAAAGGAAAG AAGCTCAGGA CTTGCTTAAT
    CACTCGGAAA AGGAAAAGAA TAATTTAGAG ATAGATTTAA ACTATAAGCT TAAATCATTA
    CAACAACGGC TAGAACAAGA GGTGAATGAA CATAAAGTAA CCAAAGCTCG TTTAACTGAC
    AAACATCAAT CTATTGAAGA AGCAAAGTCT GTTGCAATGT GTGAAATGGA AAAAAAGCTG
    AAGGAAGAGA GAGAAGCTCG AGAGAAGGCT GAAAATAGAG TTGTTCAGAT TGAGAAACAG
    TGTTCAATGC TGGATGTTGA TCTGAAACAA TCTCAGCAGA AGCTCGAACA TTTGACTGAG
    AATAAAGAAA GGATGGAGGA TGAAGTTAAG AATCTATCCC TGCAGCTGGA GCAGGAATCA
    AATAAGCGAC TATTGTTACA GAATGAATTG AAGACTCAAG CATTTGAAGC AGACAATTTA
    AAAGGTCTAG AAAAGCAGAT GAAACAGGAA ATAAATACTT TATTGGAAGC AAAGAGATTA
    TTAGAATTTG AATTAGCTCA GCTTACAAAA CAGTATAGAG GAAATGAAGG ACAGATGCGG
    GAGCTACAAG ATCAGCTTGA AGCTGAGCAG TATTTCTCGA CACTTTATAA AACCCAGGTA
    AAGGAACTTA AGGAAGAAAT TGAAGAAAAA AACAGAGAAA ATTTAAAGAA AATACAAGAG
    CTACAAAATG AAAAAGAAAC TCTTGCTACT CAGTTGGATC TGGCAGAAAC AAAAGCTGAG
    TCTGAGCAGT TGGCTCGAGG GCTTCTGGAA GAACAGTATT TTGAATTAAC CCAAGAAAGC
    AAGAAAGCTG CTTCCAGAAA TAGGCAAGAG ATTACAGATA AAGATCACAC TGTTAGTCGG
    CTTGAAGAAT CGAACAGCAT GTTAACCAAA GATAATGAAT TATTAAGAAA AGAAAATGAA
    GAGCTAACTG ATAAAATGAA GAGGGCAGAG GAAGAATATA AATTGAAGAA GGAGGAGGAG
    ATCAATAATC TTAAGGCTGC CTTTGAAAAG AATATCAATA CAGAGAGAAC CCTTAAAACG
    CAGGCTGTTA ACAAGTTGGC AGAAATAATG AATCGGAAAG ATTTTAAAAT TGATAGAAAG
    AAAGCTAACA CACAGGATTT GAGAAAGAAA GAAAAGGAAA ATCGAAAGCT CCAACTGGAA
    CTCAACCAAG AAAGAGAGAA ATTCAACCAG ATGGTGGTGA AGCATCAGAA GGAACTGAAT
    GACATGCAAG CGCAATTGGT AGAAGAATGT ACGCATAAGA ATGAGCTTCA GATGCAATTA
    GCTAGCAAAG AGAGTGATAT TGAGCAACTG CGTGCCAAAC TTTTGGACCT TTCCGATTCT
    ACAAGTATTG CTAGTTTTCC TAGTGCTGAT GAAACTGATG GAAACCTTCC AGAGTCAAGA
    ATTGAAGGTT GGCTTTCAAT ACCAAATAGA GGAAATATCA AACGATATGG CTGGAAGAAA
    CAGTATGTTG TGGTAAGCAG CAAAAAAATT TTGTTCTATA ATGATGAACA AGATAAGGAA
    CAATCCAATC CATCCATGGT ATTGGACATA GATAAATTGT TTCATGTTCG ACCTGTAACC
    CAAGGAGATG TTTACAGAGC TGAAACTGAA GAAATTCCTA AAATATTCCA GATACTATAT
    GCAAATGAAG GTGAATGTAG AAAAGATGTA GAGATGGAAC CAGTACAACA AGCTGAAAAG
    ACTAATTTTC AAAACCACAA AGGCCATGAG TTCATTCCTA CACTCTACCA CTTCCCTGCC
    AACTGTGAGG CCTGTGCCAA ACCTCTCTGG CATGTTTTTA AGCCACCTCC TGCCCTAGAA
    TGTCGAAGAT GCCATGTTAA GTGCCACAGA GATCACTTAG ATAAGAAAGA GGATTTAATT
    TCTCCATGTA AAGTAAGTTA CGATGTAACA TCAGCAAGAG ATATGCTGCT GTTAGCATGT
    TCTCAGGATG AACAAAAAAA ATGGGTAACA CATTTGGTAA AGAAGATCCC TAAGAATCCA
    CCATCTGGTT TTGTTCGTGC ATCGCCTCGA ACGCTTTCTA CAAGATCTAC TGCAAATCAG
    TCTTTCCGAA AGGTGGTCAA AAATACATCT GGAAAAACTA GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022277243.1
    CDS1..4065
    Translation

    Target ORF information:

    RefSeq Version XM_022421535.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Rho associated coiled-coil containing protein kinase 1 (ROCK1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022421535.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCGACTG GGGACAGTTT TGAGGCTCGG TTTGAAAAAA TCGACAACCT GCTGCGGGAT 
    CCGAAATCAG AAGTGAATTC GGATTGTTTG CTGGATGGAT TGGATGCTTT GGTATATGAT
    TTGGATTTTC CTGCCTTAAG AAAAAACAAA AATATTGACA ACTTTTTAAG CAGATATAAA
    GACACAATAA ATAAAATTAG AGATTTACGA ATGAAAGCTG AAGATTATGA AGTAGTGAAG
    GTGATTGGTC GAGGTGCATT TGGAGAAGTT CAATTGGTAA GGCATAAATC CACCAGGAAG
    GTATATGCTA TGAAGCTTCT CAGTAAATTT GAAATGATTA AGAGATCTGA TTCTGCTTTT
    TTCTGGGAAG AAAGGGACAT CATGGCTTTT GCTAATAGCC CTTGGGTCGT CCAGCTTTTT
    TATGCATTCC AAGATGATCG TTATCTCTAC ATGGTGATGG AATACATGCC TGGTGGAGAT
    CTTGTAAATT TAATGAGCAA CTATGATGTG CCTGAAAAAT GGGCACGATT CTATACTGCA
    GAAGTAGTAC TTGCGTTGGA TGCAATCCAT TCCATGGGTT TCATTCATAG AGATGTGAAG
    CCTGATAACA TGCTGCTGGA TAAATCTGGA CATTTGAAGT TAGCAGATTT TGGTACTTGT
    ATGAAGATGA ATAAGGAAGG TATGGTACGA TGTGATACAG CAGTTGGAAC ACCTGATTAC
    ATTTCCCCTG AAGTATTAAA ATCTCAAGGT GGTGATGGCT ATTATGGACG AGAATGTGAC
    TGGTGGTCAG TTGGGGTATT TTTATATGAA ATGCTTGTAG GTGATACACC TTTTTATGCA
    GATTCTCTGG TAGGAACTTA CAGTAAGATT ATGAACCATA AAAATTCACT TACTTTCCCT
    GATGATAATG ATATATCAAA AGAAGCAAAA AATCTTATTT GTGCCTTCCT TACTGACAGA
    GAGGTGAGAT TAGGGCGAAA TGGTGTAGAA GAAATCAAAC GACATCTCTT CTTCAAAAAT
    GATCAATGGG CTTGGGAGAC ACTCCGAGAC ACTGTAGCAC CAGTTGTACC TGATTTAAGT
    AGTGACATTG ATACAAGTAA TTTTGATGAC TTGGAAGAAG ATAAAGGAGA TGAAGAAACG
    TTTCCCATAC CTAAAGCTTT TGTTGGCAAT CAGTTACCCT TTGTAGGATT TACATATTAT
    AGCAATCGTA GATACTTATC TTCAGCAAAT CCTAATGATA ACCGAAGTAG CCCCAATGTG
    GATAAAAGCT TGCAGGAAAA TTTGCAAAAA ACAATTTATA AGCTGGAAGA ACAGCTGCAT
    AATGAAATGC AATTAAAAGA TGAAATGGAG CAGAAATGCA GAACTTCAAA CATAAAACTA
    GACAAGATAA TGAAAGAATT GGATGAAGAG GGGAATCAAA GAAGAAACCT AGAATCTACA
    GTGTCTCAGA TTGAGAAGGA GAAAATGTTG CTACAGCATA GAATTAATGA GTACCAGAGA
    AAAGCAGAAC AGGAAAATGA GAAAAGAAGG AATGTAGAAC ATGAAGTTTC TACATTAAAG
    GATCAGTTGG AAGACTTAAA AAAAGTAAAT CAGAATTCAC AGCTTGCTAA TGAGAAACTT
    GCACAATTAC AAAAACAGCT AGAAGAAGCC AATGACTTAC TTAGGACAGA ATCAGACACA
    GCTGTAAGAT TAAGGAAGAG CCACACAGAA ATGAGTAAGT CAGTTAGTCA GCTAGAATCT
    CTGAACAGAG AATTGCAAGA GAGAAACAGA ATTTTAGAGA ATTCCAAGAC ACAGATGGAC
    AAGGACTATT ACCAGCTGCA GGCTGTATTA GAAGCTGAAC GAAGAGACAG AGGTCATGAT
    TCTGAGATGA TTGGAGACCT TCAAGCTCGA ATTACATCCT TACAAGAGGA GGTGAAGCAT
    CTCAAACATA ATCTTGAAAG AGTGGAGGGA GAAAGGAAAG AAGCTCAGGA CTTGCTTAAT
    CACTCGGAAA AGGAAAAGAA TAATTTAGAG ATAGATTTAA ACTATAAGCT TAAATCATTA
    CAACAACGGC TAGAACAAGA GGTGAATGAA CATAAAGTAA CCAAAGCTCG TTTAACTGAC
    AAACATCAAT CTATTGAAGA AGCAAAGTCT GTTGCAATGT GTGAAATGGA AAAAAAGCTG
    AAGGAAGAGA GAGAAGCTCG AGAGAAGGCT GAAAATAGAG TTGTTCAGAT TGAGAAACAG
    TGTTCAATGC TGGATGTTGA TCTGAAACAA TCTCAGCAGA AGCTCGAACA TTTGACTGAG
    AATAAAGAAA GGATGGAGGA TGAAGTTAAG AATCTATCCC TGCAGCTGGA GCAGGAATCA
    AATAAGCGAC TATTGTTACA GAATGAATTG AAGACTCAAG CATTTGAAGC AGACAATTTA
    AAAGGTCTAG AAAAGCAGAT GAAACAGGAA ATAAATACTT TATTGGAAGC AAAGAGATTA
    TTAGAATTTG AATTAGCTCA GCTTACAAAA CAGTATAGAG GAAATGAAGG ACAGATGCGG
    GAGCTACAAG ATCAGCTTGA AGCTGAGCAG TATTTCTCGA CACTTTATAA AACCCAGGTA
    AAGGAACTTA AGGAAGAAAT TGAAGAAAAA AACAGAGAAA ATTTAAAGAA AATACAAGAG
    CTACAAAATG AAAAAGAAAC TCTTGCTACT CAGTTGGATC TGGCAGAAAC AAAAGCTGAG
    TCTGAGCAGT TGGCTCGAGG GCTTCTGGAA GAACAGTATT TTGAATTAAC CCAAGAAAGC
    AAGAAAGCTG CTTCCAGAAA TAGGCAAGAG ATTACAGATA AAGATCACAC TGTTAGTCGG
    CTTGAAGAAT CGAACAGCAT GTTAACCAAA GATAATGAAT TATTAAGAAA AGAAAATGAA
    GAGCTAACTG ATAAAATGAA GAGGGCAGAG GAAGAATATA AATTGAAGAA GGAGGAGGAG
    ATCAATAATC TTAAGGCTGC CTTTGAAAAG AATATCAATA CAGAGAGAAC CCTTAAAACG
    CAGGCTGTTA ACAAGTTGGC AGAAATAATG AATCGGAAAG ATTTTAAAAT TGATAGAAAG
    AAAGCTAACA CACAGGATTT GAGAAAGAAA GAAAAGGAAA ATCGAAAGCT CCAACTGGAA
    CTCAACCAAG AAAGAGAGAA ATTCAACCAG ATGGTGGTGA AGCATCAGAA GGAACTGAAT
    GACATGCAAG CGCAATTGGT AGAAGAATGT ACGCATAAGA ATGAGCTTCA GATGCAATTA
    GCTAGCAAAG AGAGTGATAT TGAGCAACTG CGTGCCAAAC TTTTGGACCT TTCCGATTCT
    ACAAGTATTG CTAGTTTTCC TAGTGCTGAT GAAACTGATG GAAACCTTCC AGAGTCAAGA
    ATTGAAGGTT GGCTTTCAAT ACCAAATAGA GGAAATATCA AACGATATGG CTGGAAGAAA
    CAGTATGTTG TGGTAAGCAG CAAAAAAATT TTGTTCTATA ATGATGAACA AGATAAGGAA
    CAATCCAATC CATCCATGGT ATTGGACATA GATAAATTGT TTCATGTTCG ACCTGTAACC
    CAAGGAGATG TTTACAGAGC TGAAACTGAA GAAATTCCTA AAATATTCCA GATACTATAT
    GCAAATGAAG GTGAATGTAG AAAAGATGTA GAGATGGAAC CAGTACAACA AGCTGAAAAG
    ACTAATTTTC AAAACCACAA AGGCCATGAG TTCATTCCTA CACTCTACCA CTTCCCTGCC
    AACTGTGAGG CCTGTGCCAA ACCTCTCTGG CATGTTTTTA AGCCACCTCC TGCCCTAGAA
    TGTCGAAGAT GCCATGTTAA GTGCCACAGA GATCACTTAG ATAAGAAAGA GGATTTAATT
    TCTCCATGTA AAGTAAGTTA CGATGTAACA TCAGCAAGAG ATATGCTGCT GTTAGCATGT
    TCTCAGGATG AACAAAAAAA ATGGGTAACA CATTTGGTAA AGAAGATCCC TAAGAATCCA
    CCATCTGGTT TTGTTCGTGC ATCGCCTCGA ACGCTTTCTA CAAGATCTAC TGCAAATCAG
    TCTTTCCGAA AGGTGGTCAA AAATACATCT GGAAAAACTA GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe289827
    Clone ID Related Accession (Same CDS sequence) XM_022421536.1
    Accession Version XM_022421536.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3858bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rho-associated protein kinase 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAAAGCTG AAGATTATGA AGTAGTGAAG GTGATTGGTC GAGGTGCATT TGGAGAAGTT 
    CAATTGGTAA GGCATAAATC CACCAGGAAG GTATATGCTA TGAAGCTTCT CAGTAAATTT
    GAAATGATTA AGAGATCTGA TTCTGCTTTT TTCTGGGAAG AAAGGGACAT CATGGCTTTT
    GCTAATAGCC CTTGGGTCGT CCAGCTTTTT TATGCATTCC AAGATGATCG TTATCTCTAC
    ATGGTGATGG AATACATGCC TGGTGGAGAT CTTGTAAATT TAATGAGCAA CTATGATGTG
    CCTGAAAAAT GGGCACGATT CTATACTGCA GAAGTAGTAC TTGCGTTGGA TGCAATCCAT
    TCCATGGGTT TCATTCATAG AGATGTGAAG CCTGATAACA TGCTGCTGGA TAAATCTGGA
    CATTTGAAGT TAGCAGATTT TGGTACTTGT ATGAAGATGA ATAAGGAAGG TATGGTACGA
    TGTGATACAG CAGTTGGAAC ACCTGATTAC ATTTCCCCTG AAGTATTAAA ATCTCAAGGT
    GGTGATGGCT ATTATGGACG AGAATGTGAC TGGTGGTCAG TTGGGGTATT TTTATATGAA
    ATGCTTGTAG GTGATACACC TTTTTATGCA GATTCTCTGG TAGGAACTTA CAGTAAGATT
    ATGAACCATA AAAATTCACT TACTTTCCCT GATGATAATG ATATATCAAA AGAAGCAAAA
    AATCTTATTT GTGCCTTCCT TACTGACAGA GAGGTGAGAT TAGGGCGAAA TGGTGTAGAA
    GAAATCAAAC GACATCTCTT CTTCAAAAAT GATCAATGGG CTTGGGAGAC ACTCCGAGAC
    ACTGTAGCAC CAGTTGTACC TGATTTAAGT AGTGACATTG ATACAAGTAA TTTTGATGAC
    TTGGAAGAAG ATAAAGGAGA TGAAGAAACG TTTCCCATAC CTAAAGCTTT TGTTGGCAAT
    CAGTTACCCT TTGTAGGATT TACATATTAT AGCAATCGTA GATACTTATC TTCAGCAAAT
    CCTAATGATA ACCGAAGTAG CCCCAATGTG GATAAAAGCT TGCAGGAAAA TTTGCAAAAA
    ACAATTTATA AGCTGGAAGA ACAGCTGCAT AATGAAATGC AATTAAAAGA TGAAATGGAG
    CAGAAATGCA GAACTTCAAA CATAAAACTA GACAAGATAA TGAAAGAATT GGATGAAGAG
    GGGAATCAAA GAAGAAACCT AGAATCTACA GTGTCTCAGA TTGAGAAGGA GAAAATGTTG
    CTACAGCATA GAATTAATGA GTACCAGAGA AAAGCAGAAC AGGAAAATGA GAAAAGAAGG
    AATGTAGAAC ATGAAGTTTC TACATTAAAG GATCAGTTGG AAGACTTAAA AAAAGTAAAT
    CAGAATTCAC AGCTTGCTAA TGAGAAACTT GCACAATTAC AAAAACAGCT AGAAGAAGCC
    AATGACTTAC TTAGGACAGA ATCAGACACA GCTGTAAGAT TAAGGAAGAG CCACACAGAA
    ATGAGTAAGT CAGTTAGTCA GCTAGAATCT CTGAACAGAG AATTGCAAGA GAGAAACAGA
    ATTTTAGAGA ATTCCAAGAC ACAGATGGAC AAGGACTATT ACCAGCTGCA GGCTGTATTA
    GAAGCTGAAC GAAGAGACAG AGGTCATGAT TCTGAGATGA TTGGAGACCT TCAAGCTCGA
    ATTACATCCT TACAAGAGGA GGTGAAGCAT CTCAAACATA ATCTTGAAAG AGTGGAGGGA
    GAAAGGAAAG AAGCTCAGGA CTTGCTTAAT CACTCGGAAA AGGAAAAGAA TAATTTAGAG
    ATAGATTTAA ACTATAAGCT TAAATCATTA CAACAACGGC TAGAACAAGA GGTGAATGAA
    CATAAAGTAA CCAAAGCTCG TTTAACTGAC AAACATCAAT CTATTGAAGA AGCAAAGTCT
    GTTGCAATGT GTGAAATGGA AAAAAAGCTG AAGGAAGAGA GAGAAGCTCG AGAGAAGGCT
    GAAAATAGAG TTGTTCAGAT TGAGAAACAG TGTTCAATGC TGGATGTTGA TCTGAAACAA
    TCTCAGCAGA AGCTCGAACA TTTGACTGAG AATAAAGAAA GGATGGAGGA TGAAGTTAAG
    AATCTATCCC TGCAGCTGGA GCAGGAATCA AATAAGCGAC TATTGTTACA GAATGAATTG
    AAGACTCAAG CATTTGAAGC AGACAATTTA AAAGGTCTAG AAAAGCAGAT GAAACAGGAA
    ATAAATACTT TATTGGAAGC AAAGAGATTA TTAGAATTTG AATTAGCTCA GCTTACAAAA
    CAGTATAGAG GAAATGAAGG ACAGATGCGG GAGCTACAAG ATCAGCTTGA AGCTGAGCAG
    TATTTCTCGA CACTTTATAA AACCCAGGTA AAGGAACTTA AGGAAGAAAT TGAAGAAAAA
    AACAGAGAAA ATTTAAAGAA AATACAAGAG CTACAAAATG AAAAAGAAAC TCTTGCTACT
    CAGTTGGATC TGGCAGAAAC AAAAGCTGAG TCTGAGCAGT TGGCTCGAGG GCTTCTGGAA
    GAACAGTATT TTGAATTAAC CCAAGAAAGC AAGAAAGCTG CTTCCAGAAA TAGGCAAGAG
    ATTACAGATA AAGATCACAC TGTTAGTCGG CTTGAAGAAT CGAACAGCAT GTTAACCAAA
    GATAATGAAT TATTAAGAAA AGAAAATGAA GAGCTAACTG ATAAAATGAA GAGGGCAGAG
    GAAGAATATA AATTGAAGAA GGAGGAGGAG ATCAATAATC TTAAGGCTGC CTTTGAAAAG
    AATATCAATA CAGAGAGAAC CCTTAAAACG CAGGCTGTTA ACAAGTTGGC AGAAATAATG
    AATCGGAAAG ATTTTAAAAT TGATAGAAAG AAAGCTAACA CACAGGATTT GAGAAAGAAA
    GAAAAGGAAA ATCGAAAGCT CCAACTGGAA CTCAACCAAG AAAGAGAGAA ATTCAACCAG
    ATGGTGGTGA AGCATCAGAA GGAACTGAAT GACATGCAAG CGCAATTGGT AGAAGAATGT
    ACGCATAAGA ATGAGCTTCA GATGCAATTA GCTAGCAAAG AGAGTGATAT TGAGCAACTG
    CGTGCCAAAC TTTTGGACCT TTCCGATTCT ACAAGTATTG CTAGTTTTCC TAGTGCTGAT
    GAAACTGATG GAAACCTTCC AGAGTCAAGA ATTGAAGGTT GGCTTTCAAT ACCAAATAGA
    GGAAATATCA AACGATATGG CTGGAAGAAA CAGTATGTTG TGGTAAGCAG CAAAAAAATT
    TTGTTCTATA ATGATGAACA AGATAAGGAA CAATCCAATC CATCCATGGT ATTGGACATA
    GATAAATTGT TTCATGTTCG ACCTGTAACC CAAGGAGATG TTTACAGAGC TGAAACTGAA
    GAAATTCCTA AAATATTCCA GATACTATAT GCAAATGAAG GTGAATGTAG AAAAGATGTA
    GAGATGGAAC CAGTACAACA AGCTGAAAAG ACTAATTTTC AAAACCACAA AGGCCATGAG
    TTCATTCCTA CACTCTACCA CTTCCCTGCC AACTGTGAGG CCTGTGCCAA ACCTCTCTGG
    CATGTTTTTA AGCCACCTCC TGCCCTAGAA TGTCGAAGAT GCCATGTTAA GTGCCACAGA
    GATCACTTAG ATAAGAAAGA GGATTTAATT TCTCCATGTA AAGTAAGTTA CGATGTAACA
    TCAGCAAGAG ATATGCTGCT GTTAGCATGT TCTCAGGATG AACAAAAAAA ATGGGTAACA
    CATTTGGTAA AGAAGATCCC TAAGAATCCA CCATCTGGTT TTGTTCGTGC ATCGCCTCGA
    ACGCTTTCTA CAAGATCTAC TGCAAATCAG TCTTTCCGAA AGGTGGTCAA AAATACATCT
    GGAAAAACTA GCAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022277244.1
    CDS119..3976
    Translation

    Target ORF information:

    RefSeq Version XM_022421536.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Rho associated coiled-coil containing protein kinase 1 (ROCK1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022421536.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAAAGCTG AAGATTATGA AGTAGTGAAG GTGATTGGTC GAGGTGCATT TGGAGAAGTT 
    CAATTGGTAA GGCATAAATC CACCAGGAAG GTATATGCTA TGAAGCTTCT CAGTAAATTT
    GAAATGATTA AGAGATCTGA TTCTGCTTTT TTCTGGGAAG AAAGGGACAT CATGGCTTTT
    GCTAATAGCC CTTGGGTCGT CCAGCTTTTT TATGCATTCC AAGATGATCG TTATCTCTAC
    ATGGTGATGG AATACATGCC TGGTGGAGAT CTTGTAAATT TAATGAGCAA CTATGATGTG
    CCTGAAAAAT GGGCACGATT CTATACTGCA GAAGTAGTAC TTGCGTTGGA TGCAATCCAT
    TCCATGGGTT TCATTCATAG AGATGTGAAG CCTGATAACA TGCTGCTGGA TAAATCTGGA
    CATTTGAAGT TAGCAGATTT TGGTACTTGT ATGAAGATGA ATAAGGAAGG TATGGTACGA
    TGTGATACAG CAGTTGGAAC ACCTGATTAC ATTTCCCCTG AAGTATTAAA ATCTCAAGGT
    GGTGATGGCT ATTATGGACG AGAATGTGAC TGGTGGTCAG TTGGGGTATT TTTATATGAA
    ATGCTTGTAG GTGATACACC TTTTTATGCA GATTCTCTGG TAGGAACTTA CAGTAAGATT
    ATGAACCATA AAAATTCACT TACTTTCCCT GATGATAATG ATATATCAAA AGAAGCAAAA
    AATCTTATTT GTGCCTTCCT TACTGACAGA GAGGTGAGAT TAGGGCGAAA TGGTGTAGAA
    GAAATCAAAC GACATCTCTT CTTCAAAAAT GATCAATGGG CTTGGGAGAC ACTCCGAGAC
    ACTGTAGCAC CAGTTGTACC TGATTTAAGT AGTGACATTG ATACAAGTAA TTTTGATGAC
    TTGGAAGAAG ATAAAGGAGA TGAAGAAACG TTTCCCATAC CTAAAGCTTT TGTTGGCAAT
    CAGTTACCCT TTGTAGGATT TACATATTAT AGCAATCGTA GATACTTATC TTCAGCAAAT
    CCTAATGATA ACCGAAGTAG CCCCAATGTG GATAAAAGCT TGCAGGAAAA TTTGCAAAAA
    ACAATTTATA AGCTGGAAGA ACAGCTGCAT AATGAAATGC AATTAAAAGA TGAAATGGAG
    CAGAAATGCA GAACTTCAAA CATAAAACTA GACAAGATAA TGAAAGAATT GGATGAAGAG
    GGGAATCAAA GAAGAAACCT AGAATCTACA GTGTCTCAGA TTGAGAAGGA GAAAATGTTG
    CTACAGCATA GAATTAATGA GTACCAGAGA AAAGCAGAAC AGGAAAATGA GAAAAGAAGG
    AATGTAGAAC ATGAAGTTTC TACATTAAAG GATCAGTTGG AAGACTTAAA AAAAGTAAAT
    CAGAATTCAC AGCTTGCTAA TGAGAAACTT GCACAATTAC AAAAACAGCT AGAAGAAGCC
    AATGACTTAC TTAGGACAGA ATCAGACACA GCTGTAAGAT TAAGGAAGAG CCACACAGAA
    ATGAGTAAGT CAGTTAGTCA GCTAGAATCT CTGAACAGAG AATTGCAAGA GAGAAACAGA
    ATTTTAGAGA ATTCCAAGAC ACAGATGGAC AAGGACTATT ACCAGCTGCA GGCTGTATTA
    GAAGCTGAAC GAAGAGACAG AGGTCATGAT TCTGAGATGA TTGGAGACCT TCAAGCTCGA
    ATTACATCCT TACAAGAGGA GGTGAAGCAT CTCAAACATA ATCTTGAAAG AGTGGAGGGA
    GAAAGGAAAG AAGCTCAGGA CTTGCTTAAT CACTCGGAAA AGGAAAAGAA TAATTTAGAG
    ATAGATTTAA ACTATAAGCT TAAATCATTA CAACAACGGC TAGAACAAGA GGTGAATGAA
    CATAAAGTAA CCAAAGCTCG TTTAACTGAC AAACATCAAT CTATTGAAGA AGCAAAGTCT
    GTTGCAATGT GTGAAATGGA AAAAAAGCTG AAGGAAGAGA GAGAAGCTCG AGAGAAGGCT
    GAAAATAGAG TTGTTCAGAT TGAGAAACAG TGTTCAATGC TGGATGTTGA TCTGAAACAA
    TCTCAGCAGA AGCTCGAACA TTTGACTGAG AATAAAGAAA GGATGGAGGA TGAAGTTAAG
    AATCTATCCC TGCAGCTGGA GCAGGAATCA AATAAGCGAC TATTGTTACA GAATGAATTG
    AAGACTCAAG CATTTGAAGC AGACAATTTA AAAGGTCTAG AAAAGCAGAT GAAACAGGAA
    ATAAATACTT TATTGGAAGC AAAGAGATTA TTAGAATTTG AATTAGCTCA GCTTACAAAA
    CAGTATAGAG GAAATGAAGG ACAGATGCGG GAGCTACAAG ATCAGCTTGA AGCTGAGCAG
    TATTTCTCGA CACTTTATAA AACCCAGGTA AAGGAACTTA AGGAAGAAAT TGAAGAAAAA
    AACAGAGAAA ATTTAAAGAA AATACAAGAG CTACAAAATG AAAAAGAAAC TCTTGCTACT
    CAGTTGGATC TGGCAGAAAC AAAAGCTGAG TCTGAGCAGT TGGCTCGAGG GCTTCTGGAA
    GAACAGTATT TTGAATTAAC CCAAGAAAGC AAGAAAGCTG CTTCCAGAAA TAGGCAAGAG
    ATTACAGATA AAGATCACAC TGTTAGTCGG CTTGAAGAAT CGAACAGCAT GTTAACCAAA
    GATAATGAAT TATTAAGAAA AGAAAATGAA GAGCTAACTG ATAAAATGAA GAGGGCAGAG
    GAAGAATATA AATTGAAGAA GGAGGAGGAG ATCAATAATC TTAAGGCTGC CTTTGAAAAG
    AATATCAATA CAGAGAGAAC CCTTAAAACG CAGGCTGTTA ACAAGTTGGC AGAAATAATG
    AATCGGAAAG ATTTTAAAAT TGATAGAAAG AAAGCTAACA CACAGGATTT GAGAAAGAAA
    GAAAAGGAAA ATCGAAAGCT CCAACTGGAA CTCAACCAAG AAAGAGAGAA ATTCAACCAG
    ATGGTGGTGA AGCATCAGAA GGAACTGAAT GACATGCAAG CGCAATTGGT AGAAGAATGT
    ACGCATAAGA ATGAGCTTCA GATGCAATTA GCTAGCAAAG AGAGTGATAT TGAGCAACTG
    CGTGCCAAAC TTTTGGACCT TTCCGATTCT ACAAGTATTG CTAGTTTTCC TAGTGCTGAT
    GAAACTGATG GAAACCTTCC AGAGTCAAGA ATTGAAGGTT GGCTTTCAAT ACCAAATAGA
    GGAAATATCA AACGATATGG CTGGAAGAAA CAGTATGTTG TGGTAAGCAG CAAAAAAATT
    TTGTTCTATA ATGATGAACA AGATAAGGAA CAATCCAATC CATCCATGGT ATTGGACATA
    GATAAATTGT TTCATGTTCG ACCTGTAACC CAAGGAGATG TTTACAGAGC TGAAACTGAA
    GAAATTCCTA AAATATTCCA GATACTATAT GCAAATGAAG GTGAATGTAG AAAAGATGTA
    GAGATGGAAC CAGTACAACA AGCTGAAAAG ACTAATTTTC AAAACCACAA AGGCCATGAG
    TTCATTCCTA CACTCTACCA CTTCCCTGCC AACTGTGAGG CCTGTGCCAA ACCTCTCTGG
    CATGTTTTTA AGCCACCTCC TGCCCTAGAA TGTCGAAGAT GCCATGTTAA GTGCCACAGA
    GATCACTTAG ATAAGAAAGA GGATTTAATT TCTCCATGTA AAGTAAGTTA CGATGTAACA
    TCAGCAAGAG ATATGCTGCT GTTAGCATGT TCTCAGGATG AACAAAAAAA ATGGGTAACA
    CATTTGGTAA AGAAGATCCC TAAGAATCCA CCATCTGGTT TTGTTCGTGC ATCGCCTCGA
    ACGCTTTCTA CAAGATCTAC TGCAAATCAG TCTTTCCGAA AGGTGGTCAA AAATACATCT
    GGAAAAACTA GCAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe09897
    Clone ID Related Accession (Same CDS sequence) XM_005623022.3 , XM_005623022.2
    Accession Version XM_005623022.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4068bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rho-associated protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005623022.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCGACTG GGGACAGTTT TGAGGCTCGG TTTGAAAAAA TCGACAACCT GCTGCGGGAT 
    CCGAAATCAG AAGTGAATTC GGATTGTTTG CTGGATGGAT TGGATGCTTT GGTATATGAT
    TTGGATTTTC CTGCCTTAAG AAAAAACAAA AATATTGACA ACTTTTTAAG CAGATATAAA
    GACACAATAA ATAAAATTAG AGATTTACGA ATGAAAGCTG AAGATTATGA AGTAGTGAAG
    GTGATTGGTC GAGGTGCATT TGGAGAAGTT CAATTGGTAA GGCATAAATC CACCAGGAAG
    GTATATGCTA TGAAGCTTCT CAGTAAATTT GAAATGATTA AGAGATCTGA TTCTGCTTTT
    TTCTGGGAAG AAAGGGACAT CATGGCTTTT GCTAATAGCC CTTGGGTCGT CCAGCTTTTT
    TATGCATTCC AAGATGATCG TTATCTCTAC ATGGTGATGG AATACATGCC TGGTGGAGAT
    CTTGTAAATT TAATGAGCAA CTATGATGTG CCTGAAAAAT GGGCACGATT CTATACTGCA
    GAAGTAGTAC TTGCGTTGGA TGCAATCCAT TCCATGGGTT TCATTCATAG AGATGTGAAG
    CCTGATAACA TGCTGCTGGA TAAATCTGGA CATTTGAAGT TAGCAGATTT TGGTACTTGT
    ATGAAGATGA ATAAGGAAGG TATGGTACGA TGTGATACAG CAGTTGGAAC ACCTGATTAC
    ATTTCCCCTG AAGTATTAAA ATCTCAAGGT GGTGATGGCT ATTATGGACG AGAATGTGAC
    TGGTGGTCAG TTGGGGTATT TTTATATGAA ATGCTTGTAG GTGATACACC TTTTTATGCA
    GATTCTCTGG TAGGAACTTA CAGTAAGATT ATGAACCATA AAAATTCACT TACTTTCCCT
    GATGATAATG ATATATCAAA AGAAGCAAAA AATCTTATTT GTGCCTTCCT TACTGACAGA
    GAGGTGAGAT TAGGGCGAAA TGGTGTAGAA GAAATCAAAC GACATCTCTT CTTCAAAAAT
    GATCAATGGG CTTGGGAGAC ACTCCGAGAC ACTGTAGCAC CAGTTGTACC TGATTTAAGT
    AGTGACATTG ATACAAGTAA TTTTGATGAC TTGGAAGAAG ATAAAGGAGA TGAAGAAACG
    TTTCCCATAC CTAAAGCTTT TGTTGGCAAT CAGTTACCCT TTGTAGGATT TACATATTAT
    AGCAATCGTA GATACTTATC TTCAGCAAAT CCTAATGATA ACCGAAGTAG CCCCAATGTG
    GATAAAAGCT TGCAGGAAAA TTTGCAAAAA ACAATTTATA AGCTGGAAGA ACAGCTGCAT
    AATGAAATGC AATTAAAAGA TGAAATGGAG CAGAAATGCA GAACTTCAAA CATAAAACTA
    GACAAGATAA TGAAAGAATT GGATGAAGAG GGGAATCAAA GAAGAAACCT AGAATCTACA
    GTGTCTCAGA TTGAGAAGGA GAAAATGTTG CTACAGCATA GAATTAATGA GTACCAGAGA
    AAAGCAGAAC AGGAAAATGA GAAAAGAAGG AATGTAGAAC ATGAAGTTTC TACATTAAAG
    GATCAGTTGG AAGACTTAAA AAAAGTAAAT CAGAATTCAC AGCTTGCTAA TGAGAAACTT
    GCACAATTAC AAAAACAGCT AGAAGAAGCC AATGACTTAC TTAGGACAGA ATCAGACACA
    GCTGTAAGAT TAAGGAAGAG CCACACAGAA ATGAGTAAGT CAGTTAGTCA GCTAGAATCT
    CTGAACAGAG AATTGCAAGA GAGAAACAGA ATTTTAGAGA ATTCCAAGAC ACAGATGGAC
    AAGGACTATT ACCAGCTGCA GGCTGTATTA GAAGCTGAAC GAAGAGACAG AGGTCATGAT
    TCTGAGATGA TTGGAGACCT TCAAGCTCGA ATTACATCCT TACAAGAGGA GGTGAAGCAT
    CTCAAACATA ATCTTGAAAG AGTGGAGGGA GAAAGGAAAG AAGCTCAGGA CTTGCTTAAT
    CACTCGGAAA AGGAAAAGAA TAATTTAGAG ATAGATTTAA ACTATAAGCT TAAATCATTA
    CAACAACGGC TAGAACAAGA GGTGAATGAA CATAAAGTAA CCAAAGCTCG TTTAACTGAC
    AAACATCAAT CTATTGAAGA AGCAAAGTCT GTTGCAATGT GTGAAATGGA AAAAAAGCTG
    AAGGAAGAGA GAGAAGCTCG AGAGAAGGCT GAAAATAGAG TTGTTCAGAT TGAGAAACAG
    TGTTCAATGC TGGATGTTGA TCTGAAACAA TCTCAGCAGA AGCTCGAACA TTTGACTGAG
    AATAAAGAAA GGATGGAGGA TGAAGTTAAG AATCTATCCC TGCAGCTGGA GCAGGAATCA
    AATAAGCGAC TATTGTTACA GAATGAATTG AAGACTCAAG CATTTGAAGC AGACAATTTA
    AAAGGTCTAG AAAAGCAGAT GAAACAGGAA ATAAATACTT TATTGGAAGC AAAGAGATTA
    TTAGAATTTG AATTAGCTCA GCTTACAAAA CAGTATAGAG GAAATGAAGG ACAGATGCGG
    GAGCTACAAG ATCAGCTTGA AGCTGAGCAG TATTTCTCGA CACTTTATAA AACCCAGGTA
    AAGGAACTTA AGGAAGAAAT TGAAGAAAAA AACAGAGAAA ATTTAAAGAA AATACAAGAG
    CTACAAAATG AAAAAGAAAC TCTTGCTACT CAGTTGGATC TGGCAGAAAC AAAAGCTGAG
    TCTGAGCAGT TGGCTCGAGG GCTTCTGGAA GAACAGTATT TTGAATTAAC CCAAGAAAGC
    AAGAAAGCTG CTTCCAGAAA TAGGCAAGAG ATTACAGATA AAGATCACAC TGTTAGTCGG
    CTTGAAGAAT CGAACAGCAT GTTAACCAAA GATAATGAAT TATTAAGAAA AGAAAATGAA
    GAGCTAACTG ATAAAATGAA GAGGGCAGAG GAAGAATATA AATTGAAGAA GGAGGAGGAG
    ATCAATAATC TTAAGGCTGC CTTTGAAAAG AATATCAATA CAGAGAGAAC CCTTAAAACG
    CAGGCTGTTA ACAAGTTGGC AGAAATAATG AATCGGAAAG ATTTTAAAAT TGATAGAAAG
    AAAGCTAACA CACAGGATTT GAGAAAGAAA GAAAAGGAAA ATCGAAAGCT CCAACTGGAA
    CTCAACCAAG AAAGAGAGAA ATTCAACCAG ATGGTGGTGA AGCATCAGAA GGAACTGAAT
    GACATGCAAG CGCAATTGGT AGAAGAATGT ACGCATAAGA ATGAGCTTCA GATGCAATTA
    GCTAGCAAAG AGAGTGATAT TGAGCAACTG CGTGCCAAAC TTTTGGACCT TTCCGATTCT
    ACAAGTATTG CTAGTTTTCC TAGTGCTGAT GAAACTGATG GAAACCTTCC AGAGTCAAGA
    ATTGAAGGTT GGCTTTCAAT ACCAAATAGA GGAAATATCA AACGATATGG CTGGAAGAAA
    CAGTATGTTG TGGTAAGCAG CAAAAAAATT TTGTTCTATA ATGATGAACA AGATAAGGAA
    CAATCCAATC CATCCATGGT ATTGGACATA GATAAATTGT TTCATGTTCG ACCTGTAACC
    CAAGGAGATG TTTACAGAGC TGAAACTGAA GAAATTCCTA AAATATTCCA GATACTATAT
    GCAAATGAAG GTGAATGTAG AAAAGATGTA GAGATGGAAC CAGTACAACA AGCTGAAAAG
    ACTAATTTTC AAAACCACAA AGGCCATGAG TTCATTCCTA CACTCTACCA CTTCCCTGCC
    AACTGTGAGG CCTGTGCCAA ACCTCTCTGG CATGTTTTTA AGCCACCTCC TGCCCTAGAA
    TGTCGAAGAT GCCATGTTAA GTGCCACAGA GATCACTTAG ATAAGAAAGA GGATTTAATT
    TCTCCATGTA AAGTAAGTTA CGATGTAACA TCAGCAAGAG ATATGCTGCT GTTAGCATGT
    TCTCAGGATG AACAAAAAAA ATGGGTAACA CATTTGGTAA AGAAGATCCC TAAGAATCCA
    CCATCTGGTT TTGTTCGTGC ATCGCCTCGA ACGCTTTCTA CAAGATCTAC TGCAAATCAG
    TCTTTCCGAA AGGTGGTCAA AAATACATCT GGAAAAACTA GCAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005623079.1
    CDS849..4916
    Translation

    Target ORF information:

    RefSeq Version XM_005623022.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Rho associated coiled-coil containing protein kinase 1 (ROCK1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005623022.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCGACTG GGGACAGTTT TGAGGCTCGG TTTGAAAAAA TCGACAACCT GCTGCGGGAT 
    CCGAAATCAG AAGTGAATTC GGATTGTTTG CTGGATGGAT TGGATGCTTT GGTATATGAT
    TTGGATTTTC CTGCCTTAAG AAAAAACAAA AATATTGACA ACTTTTTAAG CAGATATAAA
    GACACAATAA ATAAAATTAG AGATTTACGA ATGAAAGCTG AAGATTATGA AGTAGTGAAG
    GTGATTGGTC GAGGTGCATT TGGAGAAGTT CAATTGGTAA GGCATAAATC CACCAGGAAG
    GTATATGCTA TGAAGCTTCT CAGTAAATTT GAAATGATTA AGAGATCTGA TTCTGCTTTT
    TTCTGGGAAG AAAGGGACAT CATGGCTTTT GCTAATAGCC CTTGGGTCGT CCAGCTTTTT
    TATGCATTCC AAGATGATCG TTATCTCTAC ATGGTGATGG AATACATGCC TGGTGGAGAT
    CTTGTAAATT TAATGAGCAA CTATGATGTG CCTGAAAAAT GGGCACGATT CTATACTGCA
    GAAGTAGTAC TTGCGTTGGA TGCAATCCAT TCCATGGGTT TCATTCATAG AGATGTGAAG
    CCTGATAACA TGCTGCTGGA TAAATCTGGA CATTTGAAGT TAGCAGATTT TGGTACTTGT
    ATGAAGATGA ATAAGGAAGG TATGGTACGA TGTGATACAG CAGTTGGAAC ACCTGATTAC
    ATTTCCCCTG AAGTATTAAA ATCTCAAGGT GGTGATGGCT ATTATGGACG AGAATGTGAC
    TGGTGGTCAG TTGGGGTATT TTTATATGAA ATGCTTGTAG GTGATACACC TTTTTATGCA
    GATTCTCTGG TAGGAACTTA CAGTAAGATT ATGAACCATA AAAATTCACT TACTTTCCCT
    GATGATAATG ATATATCAAA AGAAGCAAAA AATCTTATTT GTGCCTTCCT TACTGACAGA
    GAGGTGAGAT TAGGGCGAAA TGGTGTAGAA GAAATCAAAC GACATCTCTT CTTCAAAAAT
    GATCAATGGG CTTGGGAGAC ACTCCGAGAC ACTGTAGCAC CAGTTGTACC TGATTTAAGT
    AGTGACATTG ATACAAGTAA TTTTGATGAC TTGGAAGAAG ATAAAGGAGA TGAAGAAACG
    TTTCCCATAC CTAAAGCTTT TGTTGGCAAT CAGTTACCCT TTGTAGGATT TACATATTAT
    AGCAATCGTA GATACTTATC TTCAGCAAAT CCTAATGATA ACCGAAGTAG CCCCAATGTG
    GATAAAAGCT TGCAGGAAAA TTTGCAAAAA ACAATTTATA AGCTGGAAGA ACAGCTGCAT
    AATGAAATGC AATTAAAAGA TGAAATGGAG CAGAAATGCA GAACTTCAAA CATAAAACTA
    GACAAGATAA TGAAAGAATT GGATGAAGAG GGGAATCAAA GAAGAAACCT AGAATCTACA
    GTGTCTCAGA TTGAGAAGGA GAAAATGTTG CTACAGCATA GAATTAATGA GTACCAGAGA
    AAAGCAGAAC AGGAAAATGA GAAAAGAAGG AATGTAGAAC ATGAAGTTTC TACATTAAAG
    GATCAGTTGG AAGACTTAAA AAAAGTAAAT CAGAATTCAC AGCTTGCTAA TGAGAAACTT
    GCACAATTAC AAAAACAGCT AGAAGAAGCC AATGACTTAC TTAGGACAGA ATCAGACACA
    GCTGTAAGAT TAAGGAAGAG CCACACAGAA ATGAGTAAGT CAGTTAGTCA GCTAGAATCT
    CTGAACAGAG AATTGCAAGA GAGAAACAGA ATTTTAGAGA ATTCCAAGAC ACAGATGGAC
    AAGGACTATT ACCAGCTGCA GGCTGTATTA GAAGCTGAAC GAAGAGACAG AGGTCATGAT
    TCTGAGATGA TTGGAGACCT TCAAGCTCGA ATTACATCCT TACAAGAGGA GGTGAAGCAT
    CTCAAACATA ATCTTGAAAG AGTGGAGGGA GAAAGGAAAG AAGCTCAGGA CTTGCTTAAT
    CACTCGGAAA AGGAAAAGAA TAATTTAGAG ATAGATTTAA ACTATAAGCT TAAATCATTA
    CAACAACGGC TAGAACAAGA GGTGAATGAA CATAAAGTAA CCAAAGCTCG TTTAACTGAC
    AAACATCAAT CTATTGAAGA AGCAAAGTCT GTTGCAATGT GTGAAATGGA AAAAAAGCTG
    AAGGAAGAGA GAGAAGCTCG AGAGAAGGCT GAAAATAGAG TTGTTCAGAT TGAGAAACAG
    TGTTCAATGC TGGATGTTGA TCTGAAACAA TCTCAGCAGA AGCTCGAACA TTTGACTGAG
    AATAAAGAAA GGATGGAGGA TGAAGTTAAG AATCTATCCC TGCAGCTGGA GCAGGAATCA
    AATAAGCGAC TATTGTTACA GAATGAATTG AAGACTCAAG CATTTGAAGC AGACAATTTA
    AAAGGTCTAG AAAAGCAGAT GAAACAGGAA ATAAATACTT TATTGGAAGC AAAGAGATTA
    TTAGAATTTG AATTAGCTCA GCTTACAAAA CAGTATAGAG GAAATGAAGG ACAGATGCGG
    GAGCTACAAG ATCAGCTTGA AGCTGAGCAG TATTTCTCGA CACTTTATAA AACCCAGGTA
    AAGGAACTTA AGGAAGAAAT TGAAGAAAAA AACAGAGAAA ATTTAAAGAA AATACAAGAG
    CTACAAAATG AAAAAGAAAC TCTTGCTACT CAGTTGGATC TGGCAGAAAC AAAAGCTGAG
    TCTGAGCAGT TGGCTCGAGG GCTTCTGGAA GAACAGTATT TTGAATTAAC CCAAGAAAGC
    AAGAAAGCTG CTTCCAGAAA TAGGCAAGAG ATTACAGATA AAGATCACAC TGTTAGTCGG
    CTTGAAGAAT CGAACAGCAT GTTAACCAAA GATAATGAAT TATTAAGAAA AGAAAATGAA
    GAGCTAACTG ATAAAATGAA GAGGGCAGAG GAAGAATATA AATTGAAGAA GGAGGAGGAG
    ATCAATAATC TTAAGGCTGC CTTTGAAAAG AATATCAATA CAGAGAGAAC CCTTAAAACG
    CAGGCTGTTA ACAAGTTGGC AGAAATAATG AATCGGAAAG ATTTTAAAAT TGATAGAAAG
    AAAGCTAACA CACAGGATTT GAGAAAGAAA GAAAAGGAAA ATCGAAAGCT CCAACTGGAA
    CTCAACCAAG AAAGAGAGAA ATTCAACCAG ATGGTGGTGA AGCATCAGAA GGAACTGAAT
    GACATGCAAG CGCAATTGGT AGAAGAATGT ACGCATAAGA ATGAGCTTCA GATGCAATTA
    GCTAGCAAAG AGAGTGATAT TGAGCAACTG CGTGCCAAAC TTTTGGACCT TTCCGATTCT
    ACAAGTATTG CTAGTTTTCC TAGTGCTGAT GAAACTGATG GAAACCTTCC AGAGTCAAGA
    ATTGAAGGTT GGCTTTCAAT ACCAAATAGA GGAAATATCA AACGATATGG CTGGAAGAAA
    CAGTATGTTG TGGTAAGCAG CAAAAAAATT TTGTTCTATA ATGATGAACA AGATAAGGAA
    CAATCCAATC CATCCATGGT ATTGGACATA GATAAATTGT TTCATGTTCG ACCTGTAACC
    CAAGGAGATG TTTACAGAGC TGAAACTGAA GAAATTCCTA AAATATTCCA GATACTATAT
    GCAAATGAAG GTGAATGTAG AAAAGATGTA GAGATGGAAC CAGTACAACA AGCTGAAAAG
    ACTAATTTTC AAAACCACAA AGGCCATGAG TTCATTCCTA CACTCTACCA CTTCCCTGCC
    AACTGTGAGG CCTGTGCCAA ACCTCTCTGG CATGTTTTTA AGCCACCTCC TGCCCTAGAA
    TGTCGAAGAT GCCATGTTAA GTGCCACAGA GATCACTTAG ATAAGAAAGA GGATTTAATT
    TCTCCATGTA AAGTAAGTTA CGATGTAACA TCAGCAAGAG ATATGCTGCT GTTAGCATGT
    TCTCAGGATG AACAAAAAAA ATGGGTAACA CATTTGGTAA AGAAGATCCC TAAGAATCCA
    CCATCTGGTT TTGTTCGTGC ATCGCCTCGA ACGCTTTCTA CAAGATCTAC TGCAAATCAG
    TCTTTCCGAA AGGTGGTCAA AAATACATCT GGAAAAACTA GCAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe09897
    Clone ID Related Accession (Same CDS sequence) XM_005623022.3 , XM_005623022.2
    Accession Version XM_005623022.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4068bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product rho-associated protein kinase 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726068.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005623022.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCGACTG GGGACAGTTT TGAGGCTCGG TTTGAAAAAA TCGACAACCT GCTGCGGGAT 
    CCGAAATCAG AAGTGAATTC GGATTGTTTG CTGGATGGAT TGGATGCTTT GGTATATGAT
    TTGGATTTTC CTGCCTTAAG AAAAAACAAA AATATTGACA ACTTTTTAAG CAGATATAAA
    GACACAATAA ATAAAATTAG AGATTTACGA ATGAAAGCTG AAGATTATGA AGTAGTGAAG
    GTGATTGGTC GAGGTGCATT TGGAGAAGTT CAATTGGTAA GGCATAAATC CACCAGGAAG
    GTATATGCTA TGAAGCTTCT CAGTAAATTT GAAATGATTA AGAGATCTGA TTCTGCTTTT
    TTCTGGGAAG AAAGGGACAT CATGGCTTTT GCTAATAGCC CTTGGGTCGT CCAGCTTTTT
    TATGCATTCC AAGATGATCG TTATCTCTAC ATGGTGATGG AATACATGCC TGGTGGAGAT
    CTTGTAAATT TAATGAGCAA CTATGATGTG CCTGAAAAAT GGGCACGATT CTATACTGCA
    GAAGTAGTAC TTGCGTTGGA TGCAATCCAT TCCATGGGTT TCATTCATAG AGATGTGAAG
    CCTGATAACA TGCTGCTGGA TAAATCTGGA CATTTGAAGT TAGCAGATTT TGGTACTTGT
    ATGAAGATGA ATAAGGAAGG TATGGTACGA TGTGATACAG CAGTTGGAAC ACCTGATTAC
    ATTTCCCCTG AAGTATTAAA ATCTCAAGGT GGTGATGGCT ATTATGGACG AGAATGTGAC
    TGGTGGTCAG TTGGGGTATT TTTATATGAA ATGCTTGTAG GTGATACACC TTTTTATGCA
    GATTCTCTGG TAGGAACTTA CAGTAAGATT ATGAACCATA AAAATTCACT TACTTTCCCT
    GATGATAATG ATATATCAAA AGAAGCAAAA AATCTTATTT GTGCCTTCCT TACTGACAGA
    GAGGTGAGAT TAGGGCGAAA TGGTGTAGAA GAAATCAAAC GACATCTCTT CTTCAAAAAT
    GATCAATGGG CTTGGGAGAC ACTCCGAGAC ACTGTAGCAC CAGTTGTACC TGATTTAAGT
    AGTGACATTG ATACAAGTAA TTTTGATGAC TTGGAAGAAG ATAAAGGAGA TGAAGAAACG
    TTTCCCATAC CTAAAGCTTT TGTTGGCAAT CAGTTACCCT TTGTAGGATT TACATATTAT
    AGCAATCGTA GATACTTATC TTCAGCAAAT CCTAATGATA ACCGAAGTAG CCCCAATGTG
    GATAAAAGCT TGCAGGAAAA TTTGCAAAAA ACAATTTATA AGCTGGAAGA ACAGCTGCAT
    AATGAAATGC AATTAAAAGA TGAAATGGAG CAGAAATGCA GAACTTCAAA CATAAAACTA
    GACAAGATAA TGAAAGAATT GGATGAAGAG GGGAATCAAA GAAGAAACCT AGAATCTACA
    GTGTCTCAGA TTGAGAAGGA GAAAATGTTG CTACAGCATA GAATTAATGA GTACCAGAGA
    AAAGCAGAAC AGGAAAATGA GAAAAGAAGG AATGTAGAAC ATGAAGTTTC TACATTAAAG
    GATCAGTTGG AAGACTTAAA AAAAGTAAAT CAGAATTCAC AGCTTGCTAA TGAGAAACTT
    GCACAATTAC AAAAACAGCT AGAAGAAGCC AATGACTTAC TTAGGACAGA ATCAGACACA
    GCTGTAAGAT TAAGGAAGAG CCACACAGAA ATGAGTAAGT CAGTTAGTCA GCTAGAATCT
    CTGAACAGAG AATTGCAAGA GAGAAACAGA ATTTTAGAGA ATTCCAAGAC ACAGATGGAC
    AAGGACTATT ACCAGCTGCA GGCTGTATTA GAAGCTGAAC GAAGAGACAG AGGTCATGAT
    TCTGAGATGA TTGGAGACCT TCAAGCTCGA ATTACATCCT TACAAGAGGA GGTGAAGCAT
    CTCAAACATA ATCTTGAAAG AGTGGAGGGA GAAAGGAAAG AAGCTCAGGA CTTGCTTAAT
    CACTCGGAAA AGGAAAAGAA TAATTTAGAG ATAGATTTAA ACTATAAGCT TAAATCATTA
    CAACAACGGC TAGAACAAGA GGTGAATGAA CATAAAGTAA CCAAAGCTCG TTTAACTGAC
    AAACATCAAT CTATTGAAGA AGCAAAGTCT GTTGCAATGT GTGAAATGGA AAAAAAGCTG
    AAGGAAGAGA GAGAAGCTCG AGAGAAGGCT GAAAATAGAG TTGTTCAGAT TGAGAAACAG
    TGTTCAATGC TGGATGTTGA TCTGAAACAA TCTCAGCAGA AGCTCGAACA TTTGACTGAG
    AATAAAGAAA GGATGGAGGA TGAAGTTAAG AATCTATCCC TGCAGCTGGA GCAGGAATCA
    AATAAGCGAC TATTGTTACA GAATGAATTG AAGACTCAAG CATTTGAAGC AGACAATTTA
    AAAGGTCTAG AAAAGCAGAT GAAACAGGAA ATAAATACTT TATTGGAAGC AAAGAGATTA
    TTAGAATTTG AATTAGCTCA GCTTACAAAA CAGTATAGAG GAAATGAAGG ACAGATGCGG
    GAGCTACAAG ATCAGCTTGA AGCTGAGCAG TATTTCTCGA CACTTTATAA AACCCAGGTA
    AAGGAACTTA AGGAAGAAAT TGAAGAAAAA AACAGAGAAA ATTTAAAGAA AATACAAGAG
    CTACAAAATG AAAAAGAAAC TCTTGCTACT CAGTTGGATC TGGCAGAAAC AAAAGCTGAG
    TCTGAGCAGT TGGCTCGAGG GCTTCTGGAA GAACAGTATT TTGAATTAAC CCAAGAAAGC
    AAGAAAGCTG CTTCCAGAAA TAGGCAAGAG ATTACAGATA AAGATCACAC TGTTAGTCGG
    CTTGAAGAAT CGAACAGCAT GTTAACCAAA GATAATGAAT TATTAAGAAA AGAAAATGAA
    GAGCTAACTG ATAAAATGAA GAGGGCAGAG GAAGAATATA AATTGAAGAA GGAGGAGGAG
    ATCAATAATC TTAAGGCTGC CTTTGAAAAG AATATCAATA CAGAGAGAAC CCTTAAAACG
    CAGGCTGTTA ACAAGTTGGC AGAAATAATG AATCGGAAAG ATTTTAAAAT TGATAGAAAG
    AAAGCTAACA CACAGGATTT GAGAAAGAAA GAAAAGGAAA ATCGAAAGCT CCAACTGGAA
    CTCAACCAAG AAAGAGAGAA ATTCAACCAG ATGGTGGTGA AGCATCAGAA GGAACTGAAT
    GACATGCAAG CGCAATTGGT AGAAGAATGT ACGCATAAGA ATGAGCTTCA GATGCAATTA
    GCTAGCAAAG AGAGTGATAT TGAGCAACTG CGTGCCAAAC TTTTGGACCT TTCCGATTCT
    ACAAGTATTG CTAGTTTTCC TAGTGCTGAT GAAACTGATG GAAACCTTCC AGAGTCAAGA
    ATTGAAGGTT GGCTTTCAAT ACCAAATAGA GGAAATATCA AACGATATGG CTGGAAGAAA
    CAGTATGTTG TGGTAAGCAG CAAAAAAATT TTGTTCTATA ATGATGAACA AGATAAGGAA
    CAATCCAATC CATCCATGGT ATTGGACATA GATAAATTGT TTCATGTTCG ACCTGTAACC
    CAAGGAGATG TTTACAGAGC TGAAACTGAA GAAATTCCTA AAATATTCCA GATACTATAT
    GCAAATGAAG GTGAATGTAG AAAAGATGTA GAGATGGAAC CAGTACAACA AGCTGAAAAG
    ACTAATTTTC AAAACCACAA AGGCCATGAG TTCATTCCTA CACTCTACCA CTTCCCTGCC
    AACTGTGAGG CCTGTGCCAA ACCTCTCTGG CATGTTTTTA AGCCACCTCC TGCCCTAGAA
    TGTCGAAGAT GCCATGTTAA GTGCCACAGA GATCACTTAG ATAAGAAAGA GGATTTAATT
    TCTCCATGTA AAGTAAGTTA CGATGTAACA TCAGCAAGAG ATATGCTGCT GTTAGCATGT
    TCTCAGGATG AACAAAAAAA ATGGGTAACA CATTTGGTAA AGAAGATCCC TAAGAATCCA
    CCATCTGGTT TTGTTCGTGC ATCGCCTCGA ACGCTTTCTA CAAGATCTAC TGCAAATCAG
    TCTTTCCGAA AGGTGGTCAA AAATACATCT GGAAAAACTA GCAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005623079.1
    CDS835..4902
    Translation

    Target ORF information:

    RefSeq Version XM_005623022.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Rho-associated, coiled-coil containing protein kinase 1 (ROCK1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005623022.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCGACTG GGGACAGTTT TGAGGCTCGG TTTGAAAAAA TCGACAACCT GCTGCGGGAT 
    CCGAAATCAG AAGTGAATTC GGATTGTTTG CTGGATGGAT TGGATGCTTT GGTATATGAT
    TTGGATTTTC CTGCCTTAAG AAAAAACAAA AATATTGACA ACTTTTTAAG CAGATATAAA
    GACACAATAA ATAAAATTAG AGATTTACGA ATGAAAGCTG AAGATTATGA AGTAGTGAAG
    GTGATTGGTC GAGGTGCATT TGGAGAAGTT CAATTGGTAA GGCATAAATC CACCAGGAAG
    GTATATGCTA TGAAGCTTCT CAGTAAATTT GAAATGATTA AGAGATCTGA TTCTGCTTTT
    TTCTGGGAAG AAAGGGACAT CATGGCTTTT GCTAATAGCC CTTGGGTCGT CCAGCTTTTT
    TATGCATTCC AAGATGATCG TTATCTCTAC ATGGTGATGG AATACATGCC TGGTGGAGAT
    CTTGTAAATT TAATGAGCAA CTATGATGTG CCTGAAAAAT GGGCACGATT CTATACTGCA
    GAAGTAGTAC TTGCGTTGGA TGCAATCCAT TCCATGGGTT TCATTCATAG AGATGTGAAG
    CCTGATAACA TGCTGCTGGA TAAATCTGGA CATTTGAAGT TAGCAGATTT TGGTACTTGT
    ATGAAGATGA ATAAGGAAGG TATGGTACGA TGTGATACAG CAGTTGGAAC ACCTGATTAC
    ATTTCCCCTG AAGTATTAAA ATCTCAAGGT GGTGATGGCT ATTATGGACG AGAATGTGAC
    TGGTGGTCAG TTGGGGTATT TTTATATGAA ATGCTTGTAG GTGATACACC TTTTTATGCA
    GATTCTCTGG TAGGAACTTA CAGTAAGATT ATGAACCATA AAAATTCACT TACTTTCCCT
    GATGATAATG ATATATCAAA AGAAGCAAAA AATCTTATTT GTGCCTTCCT TACTGACAGA
    GAGGTGAGAT TAGGGCGAAA TGGTGTAGAA GAAATCAAAC GACATCTCTT CTTCAAAAAT
    GATCAATGGG CTTGGGAGAC ACTCCGAGAC ACTGTAGCAC CAGTTGTACC TGATTTAAGT
    AGTGACATTG ATACAAGTAA TTTTGATGAC TTGGAAGAAG ATAAAGGAGA TGAAGAAACG
    TTTCCCATAC CTAAAGCTTT TGTTGGCAAT CAGTTACCCT TTGTAGGATT TACATATTAT
    AGCAATCGTA GATACTTATC TTCAGCAAAT CCTAATGATA ACCGAAGTAG CCCCAATGTG
    GATAAAAGCT TGCAGGAAAA TTTGCAAAAA ACAATTTATA AGCTGGAAGA ACAGCTGCAT
    AATGAAATGC AATTAAAAGA TGAAATGGAG CAGAAATGCA GAACTTCAAA CATAAAACTA
    GACAAGATAA TGAAAGAATT GGATGAAGAG GGGAATCAAA GAAGAAACCT AGAATCTACA
    GTGTCTCAGA TTGAGAAGGA GAAAATGTTG CTACAGCATA GAATTAATGA GTACCAGAGA
    AAAGCAGAAC AGGAAAATGA GAAAAGAAGG AATGTAGAAC ATGAAGTTTC TACATTAAAG
    GATCAGTTGG AAGACTTAAA AAAAGTAAAT CAGAATTCAC AGCTTGCTAA TGAGAAACTT
    GCACAATTAC AAAAACAGCT AGAAGAAGCC AATGACTTAC TTAGGACAGA ATCAGACACA
    GCTGTAAGAT TAAGGAAGAG CCACACAGAA ATGAGTAAGT CAGTTAGTCA GCTAGAATCT
    CTGAACAGAG AATTGCAAGA GAGAAACAGA ATTTTAGAGA ATTCCAAGAC ACAGATGGAC
    AAGGACTATT ACCAGCTGCA GGCTGTATTA GAAGCTGAAC GAAGAGACAG AGGTCATGAT
    TCTGAGATGA TTGGAGACCT TCAAGCTCGA ATTACATCCT TACAAGAGGA GGTGAAGCAT
    CTCAAACATA ATCTTGAAAG AGTGGAGGGA GAAAGGAAAG AAGCTCAGGA CTTGCTTAAT
    CACTCGGAAA AGGAAAAGAA TAATTTAGAG ATAGATTTAA ACTATAAGCT TAAATCATTA
    CAACAACGGC TAGAACAAGA GGTGAATGAA CATAAAGTAA CCAAAGCTCG TTTAACTGAC
    AAACATCAAT CTATTGAAGA AGCAAAGTCT GTTGCAATGT GTGAAATGGA AAAAAAGCTG
    AAGGAAGAGA GAGAAGCTCG AGAGAAGGCT GAAAATAGAG TTGTTCAGAT TGAGAAACAG
    TGTTCAATGC TGGATGTTGA TCTGAAACAA TCTCAGCAGA AGCTCGAACA TTTGACTGAG
    AATAAAGAAA GGATGGAGGA TGAAGTTAAG AATCTATCCC TGCAGCTGGA GCAGGAATCA
    AATAAGCGAC TATTGTTACA GAATGAATTG AAGACTCAAG CATTTGAAGC AGACAATTTA
    AAAGGTCTAG AAAAGCAGAT GAAACAGGAA ATAAATACTT TATTGGAAGC AAAGAGATTA
    TTAGAATTTG AATTAGCTCA GCTTACAAAA CAGTATAGAG GAAATGAAGG ACAGATGCGG
    GAGCTACAAG ATCAGCTTGA AGCTGAGCAG TATTTCTCGA CACTTTATAA AACCCAGGTA
    AAGGAACTTA AGGAAGAAAT TGAAGAAAAA AACAGAGAAA ATTTAAAGAA AATACAAGAG
    CTACAAAATG AAAAAGAAAC TCTTGCTACT CAGTTGGATC TGGCAGAAAC AAAAGCTGAG
    TCTGAGCAGT TGGCTCGAGG GCTTCTGGAA GAACAGTATT TTGAATTAAC CCAAGAAAGC
    AAGAAAGCTG CTTCCAGAAA TAGGCAAGAG ATTACAGATA AAGATCACAC TGTTAGTCGG
    CTTGAAGAAT CGAACAGCAT GTTAACCAAA GATAATGAAT TATTAAGAAA AGAAAATGAA
    GAGCTAACTG ATAAAATGAA GAGGGCAGAG GAAGAATATA AATTGAAGAA GGAGGAGGAG
    ATCAATAATC TTAAGGCTGC CTTTGAAAAG AATATCAATA CAGAGAGAAC CCTTAAAACG
    CAGGCTGTTA ACAAGTTGGC AGAAATAATG AATCGGAAAG ATTTTAAAAT TGATAGAAAG
    AAAGCTAACA CACAGGATTT GAGAAAGAAA GAAAAGGAAA ATCGAAAGCT CCAACTGGAA
    CTCAACCAAG AAAGAGAGAA ATTCAACCAG ATGGTGGTGA AGCATCAGAA GGAACTGAAT
    GACATGCAAG CGCAATTGGT AGAAGAATGT ACGCATAAGA ATGAGCTTCA GATGCAATTA
    GCTAGCAAAG AGAGTGATAT TGAGCAACTG CGTGCCAAAC TTTTGGACCT TTCCGATTCT
    ACAAGTATTG CTAGTTTTCC TAGTGCTGAT GAAACTGATG GAAACCTTCC AGAGTCAAGA
    ATTGAAGGTT GGCTTTCAAT ACCAAATAGA GGAAATATCA AACGATATGG CTGGAAGAAA
    CAGTATGTTG TGGTAAGCAG CAAAAAAATT TTGTTCTATA ATGATGAACA AGATAAGGAA
    CAATCCAATC CATCCATGGT ATTGGACATA GATAAATTGT TTCATGTTCG ACCTGTAACC
    CAAGGAGATG TTTACAGAGC TGAAACTGAA GAAATTCCTA AAATATTCCA GATACTATAT
    GCAAATGAAG GTGAATGTAG AAAAGATGTA GAGATGGAAC CAGTACAACA AGCTGAAAAG
    ACTAATTTTC AAAACCACAA AGGCCATGAG TTCATTCCTA CACTCTACCA CTTCCCTGCC
    AACTGTGAGG CCTGTGCCAA ACCTCTCTGG CATGTTTTTA AGCCACCTCC TGCCCTAGAA
    TGTCGAAGAT GCCATGTTAA GTGCCACAGA GATCACTTAG ATAAGAAAGA GGATTTAATT
    TCTCCATGTA AAGTAAGTTA CGATGTAACA TCAGCAAGAG ATATGCTGCT GTTAGCATGT
    TCTCAGGATG AACAAAAAAA ATGGGTAACA CATTTGGTAA AGAAGATCCC TAAGAATCCA
    CCATCTGGTT TTGTTCGTGC ATCGCCTCGA ACGCTTTCTA CAAGATCTAC TGCAAATCAG
    TCTTTCCGAA AGGTGGTCAA AAATACATCT GGAAAAACTA GCAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe09898
    Clone ID Related Accession (Same CDS sequence) XM_005623023.2 , XM_005623023.3
    Accession Version XM_005623023.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4065bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product rho-associated protein kinase 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726068.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005623023.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCGACTG GGGACAGTTT TGAGGCTCGG TTTGAAAAAA TCGACAACCT GCTGCGGGAT 
    CCGAAATCAG AAGTGAATTC GGATTGTTTG CTGGATGGAT TGGATGCTTT GGTATATGAT
    TTGGATTTTC CTGCCTTAAG AAAAAACAAA AATATTGACA ACTTTTTAAG CAGATATAAA
    GACACAATAA ATAAAATTAG AGATTTACGA ATGAAAGCTG AAGATTATGA AGTAGTGAAG
    GTGATTGGTC GAGGTGCATT TGGAGAAGTT CAATTGGTAA GGCATAAATC CACCAGGAAG
    GTATATGCTA TGAAGCTTCT CAGTAAATTT GAAATGATTA AGAGATCTGA TTCTGCTTTT
    TTCTGGGAAG AAAGGGACAT CATGGCTTTT GCTAATAGCC CTTGGGTCGT CCAGCTTTTT
    TATGCATTCC AAGATGATCG TTATCTCTAC ATGGTGATGG AATACATGCC TGGTGGAGAT
    CTTGTAAATT TAATGAGCAA CTATGATGTG CCTGAAAAAT GGGCACGATT CTATACTGCA
    GAAGTAGTAC TTGCGTTGGA TGCAATCCAT TCCATGGGTT TCATTCATAG AGATGTGAAG
    CCTGATAACA TGCTGCTGGA TAAATCTGGA CATTTGAAGT TAGCAGATTT TGGTACTTGT
    ATGAAGATGA ATAAGGAAGG TATGGTACGA TGTGATACAG CAGTTGGAAC ACCTGATTAC
    ATTTCCCCTG AAGTATTAAA ATCTCAAGGT GGTGATGGCT ATTATGGACG AGAATGTGAC
    TGGTGGTCAG TTGGGGTATT TTTATATGAA ATGCTTGTAG GTGATACACC TTTTTATGCA
    GATTCTCTGG TAGGAACTTA CAGTAAGATT ATGAACCATA AAAATTCACT TACTTTCCCT
    GATGATAATG ATATATCAAA AGAAGCAAAA AATCTTATTT GTGCCTTCCT TACTGACAGA
    GAGGTGAGAT TAGGGCGAAA TGGTGTAGAA GAAATCAAAC GACATCTCTT CTTCAAAAAT
    GATCAATGGG CTTGGGAGAC ACTCCGAGAC ACTGTAGCAC CAGTTGTACC TGATTTAAGT
    AGTGACATTG ATACAAGTAA TTTTGATGAC TTGGAAGAAG ATAAAGGAGA TGAAGAAACG
    TTTCCCATAC CTAAAGCTTT TGTTGGCAAT CAGTTACCCT TTGTAGGATT TACATATTAT
    AGCAATCGTA GATACTTATC TTCAGCAAAT CCTAATGATA ACCGAAGTAG CCCCAATGTG
    GATAAAAGCT TGGAAAATTT GCAAAAAACA ATTTATAAGC TGGAAGAACA GCTGCATAAT
    GAAATGCAAT TAAAAGATGA AATGGAGCAG AAATGCAGAA CTTCAAACAT AAAACTAGAC
    AAGATAATGA AAGAATTGGA TGAAGAGGGG AATCAAAGAA GAAACCTAGA ATCTACAGTG
    TCTCAGATTG AGAAGGAGAA AATGTTGCTA CAGCATAGAA TTAATGAGTA CCAGAGAAAA
    GCAGAACAGG AAAATGAGAA AAGAAGGAAT GTAGAACATG AAGTTTCTAC ATTAAAGGAT
    CAGTTGGAAG ACTTAAAAAA AGTAAATCAG AATTCACAGC TTGCTAATGA GAAACTTGCA
    CAATTACAAA AACAGCTAGA AGAAGCCAAT GACTTACTTA GGACAGAATC AGACACAGCT
    GTAAGATTAA GGAAGAGCCA CACAGAAATG AGTAAGTCAG TTAGTCAGCT AGAATCTCTG
    AACAGAGAAT TGCAAGAGAG AAACAGAATT TTAGAGAATT CCAAGACACA GATGGACAAG
    GACTATTACC AGCTGCAGGC TGTATTAGAA GCTGAACGAA GAGACAGAGG TCATGATTCT
    GAGATGATTG GAGACCTTCA AGCTCGAATT ACATCCTTAC AAGAGGAGGT GAAGCATCTC
    AAACATAATC TTGAAAGAGT GGAGGGAGAA AGGAAAGAAG CTCAGGACTT GCTTAATCAC
    TCGGAAAAGG AAAAGAATAA TTTAGAGATA GATTTAAACT ATAAGCTTAA ATCATTACAA
    CAACGGCTAG AACAAGAGGT GAATGAACAT AAAGTAACCA AAGCTCGTTT AACTGACAAA
    CATCAATCTA TTGAAGAAGC AAAGTCTGTT GCAATGTGTG AAATGGAAAA AAAGCTGAAG
    GAAGAGAGAG AAGCTCGAGA GAAGGCTGAA AATAGAGTTG TTCAGATTGA GAAACAGTGT
    TCAATGCTGG ATGTTGATCT GAAACAATCT CAGCAGAAGC TCGAACATTT GACTGAGAAT
    AAAGAAAGGA TGGAGGATGA AGTTAAGAAT CTATCCCTGC AGCTGGAGCA GGAATCAAAT
    AAGCGACTAT TGTTACAGAA TGAATTGAAG ACTCAAGCAT TTGAAGCAGA CAATTTAAAA
    GGTCTAGAAA AGCAGATGAA ACAGGAAATA AATACTTTAT TGGAAGCAAA GAGATTATTA
    GAATTTGAAT TAGCTCAGCT TACAAAACAG TATAGAGGAA ATGAAGGACA GATGCGGGAG
    CTACAAGATC AGCTTGAAGC TGAGCAGTAT TTCTCGACAC TTTATAAAAC CCAGGTAAAG
    GAACTTAAGG AAGAAATTGA AGAAAAAAAC AGAGAAAATT TAAAGAAAAT ACAAGAGCTA
    CAAAATGAAA AAGAAACTCT TGCTACTCAG TTGGATCTGG CAGAAACAAA AGCTGAGTCT
    GAGCAGTTGG CTCGAGGGCT TCTGGAAGAA CAGTATTTTG AATTAACCCA AGAAAGCAAG
    AAAGCTGCTT CCAGAAATAG GCAAGAGATT ACAGATAAAG ATCACACTGT TAGTCGGCTT
    GAAGAATCGA ACAGCATGTT AACCAAAGAT AATGAATTAT TAAGAAAAGA AAATGAAGAG
    CTAACTGATA AAATGAAGAG GGCAGAGGAA GAATATAAAT TGAAGAAGGA GGAGGAGATC
    AATAATCTTA AGGCTGCCTT TGAAAAGAAT ATCAATACAG AGAGAACCCT TAAAACGCAG
    GCTGTTAACA AGTTGGCAGA AATAATGAAT CGGAAAGATT TTAAAATTGA TAGAAAGAAA
    GCTAACACAC AGGATTTGAG AAAGAAAGAA AAGGAAAATC GAAAGCTCCA ACTGGAACTC
    AACCAAGAAA GAGAGAAATT CAACCAGATG GTGGTGAAGC ATCAGAAGGA ACTGAATGAC
    ATGCAAGCGC AATTGGTAGA AGAATGTACG CATAAGAATG AGCTTCAGAT GCAATTAGCT
    AGCAAAGAGA GTGATATTGA GCAACTGCGT GCCAAACTTT TGGACCTTTC CGATTCTACA
    AGTATTGCTA GTTTTCCTAG TGCTGATGAA ACTGATGGAA ACCTTCCAGA GTCAAGAATT
    GAAGGTTGGC TTTCAATACC AAATAGAGGA AATATCAAAC GATATGGCTG GAAGAAACAG
    TATGTTGTGG TAAGCAGCAA AAAAATTTTG TTCTATAATG ATGAACAAGA TAAGGAACAA
    TCCAATCCAT CCATGGTATT GGACATAGAT AAATTGTTTC ATGTTCGACC TGTAACCCAA
    GGAGATGTTT ACAGAGCTGA AACTGAAGAA ATTCCTAAAA TATTCCAGAT ACTATATGCA
    AATGAAGGTG AATGTAGAAA AGATGTAGAG ATGGAACCAG TACAACAAGC TGAAAAGACT
    AATTTTCAAA ACCACAAAGG CCATGAGTTC ATTCCTACAC TCTACCACTT CCCTGCCAAC
    TGTGAGGCCT GTGCCAAACC TCTCTGGCAT GTTTTTAAGC CACCTCCTGC CCTAGAATGT
    CGAAGATGCC ATGTTAAGTG CCACAGAGAT CACTTAGATA AGAAAGAGGA TTTAATTTCT
    CCATGTAAAG TAAGTTACGA TGTAACATCA GCAAGAGATA TGCTGCTGTT AGCATGTTCT
    CAGGATGAAC AAAAAAAATG GGTAACACAT TTGGTAAAGA AGATCCCTAA GAATCCACCA
    TCTGGTTTTG TTCGTGCATC GCCTCGAACG CTTTCTACAA GATCTACTGC AAATCAGTCT
    TTCCGAAAGG TGGTCAAAAA TACATCTGGA AAAACTAGCA GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005623080.1
    CDS835..4899
    Translation

    Target ORF information:

    RefSeq Version XM_005623023.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Rho-associated, coiled-coil containing protein kinase 1 (ROCK1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005623023.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCGACTG GGGACAGTTT TGAGGCTCGG TTTGAAAAAA TCGACAACCT GCTGCGGGAT 
    CCGAAATCAG AAGTGAATTC GGATTGTTTG CTGGATGGAT TGGATGCTTT GGTATATGAT
    TTGGATTTTC CTGCCTTAAG AAAAAACAAA AATATTGACA ACTTTTTAAG CAGATATAAA
    GACACAATAA ATAAAATTAG AGATTTACGA ATGAAAGCTG AAGATTATGA AGTAGTGAAG
    GTGATTGGTC GAGGTGCATT TGGAGAAGTT CAATTGGTAA GGCATAAATC CACCAGGAAG
    GTATATGCTA TGAAGCTTCT CAGTAAATTT GAAATGATTA AGAGATCTGA TTCTGCTTTT
    TTCTGGGAAG AAAGGGACAT CATGGCTTTT GCTAATAGCC CTTGGGTCGT CCAGCTTTTT
    TATGCATTCC AAGATGATCG TTATCTCTAC ATGGTGATGG AATACATGCC TGGTGGAGAT
    CTTGTAAATT TAATGAGCAA CTATGATGTG CCTGAAAAAT GGGCACGATT CTATACTGCA
    GAAGTAGTAC TTGCGTTGGA TGCAATCCAT TCCATGGGTT TCATTCATAG AGATGTGAAG
    CCTGATAACA TGCTGCTGGA TAAATCTGGA CATTTGAAGT TAGCAGATTT TGGTACTTGT
    ATGAAGATGA ATAAGGAAGG TATGGTACGA TGTGATACAG CAGTTGGAAC ACCTGATTAC
    ATTTCCCCTG AAGTATTAAA ATCTCAAGGT GGTGATGGCT ATTATGGACG AGAATGTGAC
    TGGTGGTCAG TTGGGGTATT TTTATATGAA ATGCTTGTAG GTGATACACC TTTTTATGCA
    GATTCTCTGG TAGGAACTTA CAGTAAGATT ATGAACCATA AAAATTCACT TACTTTCCCT
    GATGATAATG ATATATCAAA AGAAGCAAAA AATCTTATTT GTGCCTTCCT TACTGACAGA
    GAGGTGAGAT TAGGGCGAAA TGGTGTAGAA GAAATCAAAC GACATCTCTT CTTCAAAAAT
    GATCAATGGG CTTGGGAGAC ACTCCGAGAC ACTGTAGCAC CAGTTGTACC TGATTTAAGT
    AGTGACATTG ATACAAGTAA TTTTGATGAC TTGGAAGAAG ATAAAGGAGA TGAAGAAACG
    TTTCCCATAC CTAAAGCTTT TGTTGGCAAT CAGTTACCCT TTGTAGGATT TACATATTAT
    AGCAATCGTA GATACTTATC TTCAGCAAAT CCTAATGATA ACCGAAGTAG CCCCAATGTG
    GATAAAAGCT TGGAAAATTT GCAAAAAACA ATTTATAAGC TGGAAGAACA GCTGCATAAT
    GAAATGCAAT TAAAAGATGA AATGGAGCAG AAATGCAGAA CTTCAAACAT AAAACTAGAC
    AAGATAATGA AAGAATTGGA TGAAGAGGGG AATCAAAGAA GAAACCTAGA ATCTACAGTG
    TCTCAGATTG AGAAGGAGAA AATGTTGCTA CAGCATAGAA TTAATGAGTA CCAGAGAAAA
    GCAGAACAGG AAAATGAGAA AAGAAGGAAT GTAGAACATG AAGTTTCTAC ATTAAAGGAT
    CAGTTGGAAG ACTTAAAAAA AGTAAATCAG AATTCACAGC TTGCTAATGA GAAACTTGCA
    CAATTACAAA AACAGCTAGA AGAAGCCAAT GACTTACTTA GGACAGAATC AGACACAGCT
    GTAAGATTAA GGAAGAGCCA CACAGAAATG AGTAAGTCAG TTAGTCAGCT AGAATCTCTG
    AACAGAGAAT TGCAAGAGAG AAACAGAATT TTAGAGAATT CCAAGACACA GATGGACAAG
    GACTATTACC AGCTGCAGGC TGTATTAGAA GCTGAACGAA GAGACAGAGG TCATGATTCT
    GAGATGATTG GAGACCTTCA AGCTCGAATT ACATCCTTAC AAGAGGAGGT GAAGCATCTC
    AAACATAATC TTGAAAGAGT GGAGGGAGAA AGGAAAGAAG CTCAGGACTT GCTTAATCAC
    TCGGAAAAGG AAAAGAATAA TTTAGAGATA GATTTAAACT ATAAGCTTAA ATCATTACAA
    CAACGGCTAG AACAAGAGGT GAATGAACAT AAAGTAACCA AAGCTCGTTT AACTGACAAA
    CATCAATCTA TTGAAGAAGC AAAGTCTGTT GCAATGTGTG AAATGGAAAA AAAGCTGAAG
    GAAGAGAGAG AAGCTCGAGA GAAGGCTGAA AATAGAGTTG TTCAGATTGA GAAACAGTGT
    TCAATGCTGG ATGTTGATCT GAAACAATCT CAGCAGAAGC TCGAACATTT GACTGAGAAT
    AAAGAAAGGA TGGAGGATGA AGTTAAGAAT CTATCCCTGC AGCTGGAGCA GGAATCAAAT
    AAGCGACTAT TGTTACAGAA TGAATTGAAG ACTCAAGCAT TTGAAGCAGA CAATTTAAAA
    GGTCTAGAAA AGCAGATGAA ACAGGAAATA AATACTTTAT TGGAAGCAAA GAGATTATTA
    GAATTTGAAT TAGCTCAGCT TACAAAACAG TATAGAGGAA ATGAAGGACA GATGCGGGAG
    CTACAAGATC AGCTTGAAGC TGAGCAGTAT TTCTCGACAC TTTATAAAAC CCAGGTAAAG
    GAACTTAAGG AAGAAATTGA AGAAAAAAAC AGAGAAAATT TAAAGAAAAT ACAAGAGCTA
    CAAAATGAAA AAGAAACTCT TGCTACTCAG TTGGATCTGG CAGAAACAAA AGCTGAGTCT
    GAGCAGTTGG CTCGAGGGCT TCTGGAAGAA CAGTATTTTG AATTAACCCA AGAAAGCAAG
    AAAGCTGCTT CCAGAAATAG GCAAGAGATT ACAGATAAAG ATCACACTGT TAGTCGGCTT
    GAAGAATCGA ACAGCATGTT AACCAAAGAT AATGAATTAT TAAGAAAAGA AAATGAAGAG
    CTAACTGATA AAATGAAGAG GGCAGAGGAA GAATATAAAT TGAAGAAGGA GGAGGAGATC
    AATAATCTTA AGGCTGCCTT TGAAAAGAAT ATCAATACAG AGAGAACCCT TAAAACGCAG
    GCTGTTAACA AGTTGGCAGA AATAATGAAT CGGAAAGATT TTAAAATTGA TAGAAAGAAA
    GCTAACACAC AGGATTTGAG AAAGAAAGAA AAGGAAAATC GAAAGCTCCA ACTGGAACTC
    AACCAAGAAA GAGAGAAATT CAACCAGATG GTGGTGAAGC ATCAGAAGGA ACTGAATGAC
    ATGCAAGCGC AATTGGTAGA AGAATGTACG CATAAGAATG AGCTTCAGAT GCAATTAGCT
    AGCAAAGAGA GTGATATTGA GCAACTGCGT GCCAAACTTT TGGACCTTTC CGATTCTACA
    AGTATTGCTA GTTTTCCTAG TGCTGATGAA ACTGATGGAA ACCTTCCAGA GTCAAGAATT
    GAAGGTTGGC TTTCAATACC AAATAGAGGA AATATCAAAC GATATGGCTG GAAGAAACAG
    TATGTTGTGG TAAGCAGCAA AAAAATTTTG TTCTATAATG ATGAACAAGA TAAGGAACAA
    TCCAATCCAT CCATGGTATT GGACATAGAT AAATTGTTTC ATGTTCGACC TGTAACCCAA
    GGAGATGTTT ACAGAGCTGA AACTGAAGAA ATTCCTAAAA TATTCCAGAT ACTATATGCA
    AATGAAGGTG AATGTAGAAA AGATGTAGAG ATGGAACCAG TACAACAAGC TGAAAAGACT
    AATTTTCAAA ACCACAAAGG CCATGAGTTC ATTCCTACAC TCTACCACTT CCCTGCCAAC
    TGTGAGGCCT GTGCCAAACC TCTCTGGCAT GTTTTTAAGC CACCTCCTGC CCTAGAATGT
    CGAAGATGCC ATGTTAAGTG CCACAGAGAT CACTTAGATA AGAAAGAGGA TTTAATTTCT
    CCATGTAAAG TAAGTTACGA TGTAACATCA GCAAGAGATA TGCTGCTGTT AGCATGTTCT
    CAGGATGAAC AAAAAAAATG GGTAACACAT TTGGTAAAGA AGATCCCTAA GAATCCACCA
    TCTGGTTTTG TTCGTGCATC GCCTCGAACG CTTTCTACAA GATCTACTGC AAATCAGTCT
    TTCCGAAAGG TGGTCAAAAA TACATCTGGA AAAACTAGCA GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe09898
    Clone ID Related Accession (Same CDS sequence) XM_005623023.2 , XM_005623023.3
    Accession Version XM_005623023.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4065bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rho-associated protein kinase 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005623023.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCGACTG GGGACAGTTT TGAGGCTCGG TTTGAAAAAA TCGACAACCT GCTGCGGGAT 
    CCGAAATCAG AAGTGAATTC GGATTGTTTG CTGGATGGAT TGGATGCTTT GGTATATGAT
    TTGGATTTTC CTGCCTTAAG AAAAAACAAA AATATTGACA ACTTTTTAAG CAGATATAAA
    GACACAATAA ATAAAATTAG AGATTTACGA ATGAAAGCTG AAGATTATGA AGTAGTGAAG
    GTGATTGGTC GAGGTGCATT TGGAGAAGTT CAATTGGTAA GGCATAAATC CACCAGGAAG
    GTATATGCTA TGAAGCTTCT CAGTAAATTT GAAATGATTA AGAGATCTGA TTCTGCTTTT
    TTCTGGGAAG AAAGGGACAT CATGGCTTTT GCTAATAGCC CTTGGGTCGT CCAGCTTTTT
    TATGCATTCC AAGATGATCG TTATCTCTAC ATGGTGATGG AATACATGCC TGGTGGAGAT
    CTTGTAAATT TAATGAGCAA CTATGATGTG CCTGAAAAAT GGGCACGATT CTATACTGCA
    GAAGTAGTAC TTGCGTTGGA TGCAATCCAT TCCATGGGTT TCATTCATAG AGATGTGAAG
    CCTGATAACA TGCTGCTGGA TAAATCTGGA CATTTGAAGT TAGCAGATTT TGGTACTTGT
    ATGAAGATGA ATAAGGAAGG TATGGTACGA TGTGATACAG CAGTTGGAAC ACCTGATTAC
    ATTTCCCCTG AAGTATTAAA ATCTCAAGGT GGTGATGGCT ATTATGGACG AGAATGTGAC
    TGGTGGTCAG TTGGGGTATT TTTATATGAA ATGCTTGTAG GTGATACACC TTTTTATGCA
    GATTCTCTGG TAGGAACTTA CAGTAAGATT ATGAACCATA AAAATTCACT TACTTTCCCT
    GATGATAATG ATATATCAAA AGAAGCAAAA AATCTTATTT GTGCCTTCCT TACTGACAGA
    GAGGTGAGAT TAGGGCGAAA TGGTGTAGAA GAAATCAAAC GACATCTCTT CTTCAAAAAT
    GATCAATGGG CTTGGGAGAC ACTCCGAGAC ACTGTAGCAC CAGTTGTACC TGATTTAAGT
    AGTGACATTG ATACAAGTAA TTTTGATGAC TTGGAAGAAG ATAAAGGAGA TGAAGAAACG
    TTTCCCATAC CTAAAGCTTT TGTTGGCAAT CAGTTACCCT TTGTAGGATT TACATATTAT
    AGCAATCGTA GATACTTATC TTCAGCAAAT CCTAATGATA ACCGAAGTAG CCCCAATGTG
    GATAAAAGCT TGGAAAATTT GCAAAAAACA ATTTATAAGC TGGAAGAACA GCTGCATAAT
    GAAATGCAAT TAAAAGATGA AATGGAGCAG AAATGCAGAA CTTCAAACAT AAAACTAGAC
    AAGATAATGA AAGAATTGGA TGAAGAGGGG AATCAAAGAA GAAACCTAGA ATCTACAGTG
    TCTCAGATTG AGAAGGAGAA AATGTTGCTA CAGCATAGAA TTAATGAGTA CCAGAGAAAA
    GCAGAACAGG AAAATGAGAA AAGAAGGAAT GTAGAACATG AAGTTTCTAC ATTAAAGGAT
    CAGTTGGAAG ACTTAAAAAA AGTAAATCAG AATTCACAGC TTGCTAATGA GAAACTTGCA
    CAATTACAAA AACAGCTAGA AGAAGCCAAT GACTTACTTA GGACAGAATC AGACACAGCT
    GTAAGATTAA GGAAGAGCCA CACAGAAATG AGTAAGTCAG TTAGTCAGCT AGAATCTCTG
    AACAGAGAAT TGCAAGAGAG AAACAGAATT TTAGAGAATT CCAAGACACA GATGGACAAG
    GACTATTACC AGCTGCAGGC TGTATTAGAA GCTGAACGAA GAGACAGAGG TCATGATTCT
    GAGATGATTG GAGACCTTCA AGCTCGAATT ACATCCTTAC AAGAGGAGGT GAAGCATCTC
    AAACATAATC TTGAAAGAGT GGAGGGAGAA AGGAAAGAAG CTCAGGACTT GCTTAATCAC
    TCGGAAAAGG AAAAGAATAA TTTAGAGATA GATTTAAACT ATAAGCTTAA ATCATTACAA
    CAACGGCTAG AACAAGAGGT GAATGAACAT AAAGTAACCA AAGCTCGTTT AACTGACAAA
    CATCAATCTA TTGAAGAAGC AAAGTCTGTT GCAATGTGTG AAATGGAAAA AAAGCTGAAG
    GAAGAGAGAG AAGCTCGAGA GAAGGCTGAA AATAGAGTTG TTCAGATTGA GAAACAGTGT
    TCAATGCTGG ATGTTGATCT GAAACAATCT CAGCAGAAGC TCGAACATTT GACTGAGAAT
    AAAGAAAGGA TGGAGGATGA AGTTAAGAAT CTATCCCTGC AGCTGGAGCA GGAATCAAAT
    AAGCGACTAT TGTTACAGAA TGAATTGAAG ACTCAAGCAT TTGAAGCAGA CAATTTAAAA
    GGTCTAGAAA AGCAGATGAA ACAGGAAATA AATACTTTAT TGGAAGCAAA GAGATTATTA
    GAATTTGAAT TAGCTCAGCT TACAAAACAG TATAGAGGAA ATGAAGGACA GATGCGGGAG
    CTACAAGATC AGCTTGAAGC TGAGCAGTAT TTCTCGACAC TTTATAAAAC CCAGGTAAAG
    GAACTTAAGG AAGAAATTGA AGAAAAAAAC AGAGAAAATT TAAAGAAAAT ACAAGAGCTA
    CAAAATGAAA AAGAAACTCT TGCTACTCAG TTGGATCTGG CAGAAACAAA AGCTGAGTCT
    GAGCAGTTGG CTCGAGGGCT TCTGGAAGAA CAGTATTTTG AATTAACCCA AGAAAGCAAG
    AAAGCTGCTT CCAGAAATAG GCAAGAGATT ACAGATAAAG ATCACACTGT TAGTCGGCTT
    GAAGAATCGA ACAGCATGTT AACCAAAGAT AATGAATTAT TAAGAAAAGA AAATGAAGAG
    CTAACTGATA AAATGAAGAG GGCAGAGGAA GAATATAAAT TGAAGAAGGA GGAGGAGATC
    AATAATCTTA AGGCTGCCTT TGAAAAGAAT ATCAATACAG AGAGAACCCT TAAAACGCAG
    GCTGTTAACA AGTTGGCAGA AATAATGAAT CGGAAAGATT TTAAAATTGA TAGAAAGAAA
    GCTAACACAC AGGATTTGAG AAAGAAAGAA AAGGAAAATC GAAAGCTCCA ACTGGAACTC
    AACCAAGAAA GAGAGAAATT CAACCAGATG GTGGTGAAGC ATCAGAAGGA ACTGAATGAC
    ATGCAAGCGC AATTGGTAGA AGAATGTACG CATAAGAATG AGCTTCAGAT GCAATTAGCT
    AGCAAAGAGA GTGATATTGA GCAACTGCGT GCCAAACTTT TGGACCTTTC CGATTCTACA
    AGTATTGCTA GTTTTCCTAG TGCTGATGAA ACTGATGGAA ACCTTCCAGA GTCAAGAATT
    GAAGGTTGGC TTTCAATACC AAATAGAGGA AATATCAAAC GATATGGCTG GAAGAAACAG
    TATGTTGTGG TAAGCAGCAA AAAAATTTTG TTCTATAATG ATGAACAAGA TAAGGAACAA
    TCCAATCCAT CCATGGTATT GGACATAGAT AAATTGTTTC ATGTTCGACC TGTAACCCAA
    GGAGATGTTT ACAGAGCTGA AACTGAAGAA ATTCCTAAAA TATTCCAGAT ACTATATGCA
    AATGAAGGTG AATGTAGAAA AGATGTAGAG ATGGAACCAG TACAACAAGC TGAAAAGACT
    AATTTTCAAA ACCACAAAGG CCATGAGTTC ATTCCTACAC TCTACCACTT CCCTGCCAAC
    TGTGAGGCCT GTGCCAAACC TCTCTGGCAT GTTTTTAAGC CACCTCCTGC CCTAGAATGT
    CGAAGATGCC ATGTTAAGTG CCACAGAGAT CACTTAGATA AGAAAGAGGA TTTAATTTCT
    CCATGTAAAG TAAGTTACGA TGTAACATCA GCAAGAGATA TGCTGCTGTT AGCATGTTCT
    CAGGATGAAC AAAAAAAATG GGTAACACAT TTGGTAAAGA AGATCCCTAA GAATCCACCA
    TCTGGTTTTG TTCGTGCATC GCCTCGAACG CTTTCTACAA GATCTACTGC AAATCAGTCT
    TTCCGAAAGG TGGTCAAAAA TACATCTGGA AAAACTAGCA GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005623080.1
    CDS849..4913
    Translation

    Target ORF information:

    RefSeq Version XM_005623023.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Rho associated coiled-coil containing protein kinase 1 (ROCK1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005623023.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCGACTG GGGACAGTTT TGAGGCTCGG TTTGAAAAAA TCGACAACCT GCTGCGGGAT 
    CCGAAATCAG AAGTGAATTC GGATTGTTTG CTGGATGGAT TGGATGCTTT GGTATATGAT
    TTGGATTTTC CTGCCTTAAG AAAAAACAAA AATATTGACA ACTTTTTAAG CAGATATAAA
    GACACAATAA ATAAAATTAG AGATTTACGA ATGAAAGCTG AAGATTATGA AGTAGTGAAG
    GTGATTGGTC GAGGTGCATT TGGAGAAGTT CAATTGGTAA GGCATAAATC CACCAGGAAG
    GTATATGCTA TGAAGCTTCT CAGTAAATTT GAAATGATTA AGAGATCTGA TTCTGCTTTT
    TTCTGGGAAG AAAGGGACAT CATGGCTTTT GCTAATAGCC CTTGGGTCGT CCAGCTTTTT
    TATGCATTCC AAGATGATCG TTATCTCTAC ATGGTGATGG AATACATGCC TGGTGGAGAT
    CTTGTAAATT TAATGAGCAA CTATGATGTG CCTGAAAAAT GGGCACGATT CTATACTGCA
    GAAGTAGTAC TTGCGTTGGA TGCAATCCAT TCCATGGGTT TCATTCATAG AGATGTGAAG
    CCTGATAACA TGCTGCTGGA TAAATCTGGA CATTTGAAGT TAGCAGATTT TGGTACTTGT
    ATGAAGATGA ATAAGGAAGG TATGGTACGA TGTGATACAG CAGTTGGAAC ACCTGATTAC
    ATTTCCCCTG AAGTATTAAA ATCTCAAGGT GGTGATGGCT ATTATGGACG AGAATGTGAC
    TGGTGGTCAG TTGGGGTATT TTTATATGAA ATGCTTGTAG GTGATACACC TTTTTATGCA
    GATTCTCTGG TAGGAACTTA CAGTAAGATT ATGAACCATA AAAATTCACT TACTTTCCCT
    GATGATAATG ATATATCAAA AGAAGCAAAA AATCTTATTT GTGCCTTCCT TACTGACAGA
    GAGGTGAGAT TAGGGCGAAA TGGTGTAGAA GAAATCAAAC GACATCTCTT CTTCAAAAAT
    GATCAATGGG CTTGGGAGAC ACTCCGAGAC ACTGTAGCAC CAGTTGTACC TGATTTAAGT
    AGTGACATTG ATACAAGTAA TTTTGATGAC TTGGAAGAAG ATAAAGGAGA TGAAGAAACG
    TTTCCCATAC CTAAAGCTTT TGTTGGCAAT CAGTTACCCT TTGTAGGATT TACATATTAT
    AGCAATCGTA GATACTTATC TTCAGCAAAT CCTAATGATA ACCGAAGTAG CCCCAATGTG
    GATAAAAGCT TGGAAAATTT GCAAAAAACA ATTTATAAGC TGGAAGAACA GCTGCATAAT
    GAAATGCAAT TAAAAGATGA AATGGAGCAG AAATGCAGAA CTTCAAACAT AAAACTAGAC
    AAGATAATGA AAGAATTGGA TGAAGAGGGG AATCAAAGAA GAAACCTAGA ATCTACAGTG
    TCTCAGATTG AGAAGGAGAA AATGTTGCTA CAGCATAGAA TTAATGAGTA CCAGAGAAAA
    GCAGAACAGG AAAATGAGAA AAGAAGGAAT GTAGAACATG AAGTTTCTAC ATTAAAGGAT
    CAGTTGGAAG ACTTAAAAAA AGTAAATCAG AATTCACAGC TTGCTAATGA GAAACTTGCA
    CAATTACAAA AACAGCTAGA AGAAGCCAAT GACTTACTTA GGACAGAATC AGACACAGCT
    GTAAGATTAA GGAAGAGCCA CACAGAAATG AGTAAGTCAG TTAGTCAGCT AGAATCTCTG
    AACAGAGAAT TGCAAGAGAG AAACAGAATT TTAGAGAATT CCAAGACACA GATGGACAAG
    GACTATTACC AGCTGCAGGC TGTATTAGAA GCTGAACGAA GAGACAGAGG TCATGATTCT
    GAGATGATTG GAGACCTTCA AGCTCGAATT ACATCCTTAC AAGAGGAGGT GAAGCATCTC
    AAACATAATC TTGAAAGAGT GGAGGGAGAA AGGAAAGAAG CTCAGGACTT GCTTAATCAC
    TCGGAAAAGG AAAAGAATAA TTTAGAGATA GATTTAAACT ATAAGCTTAA ATCATTACAA
    CAACGGCTAG AACAAGAGGT GAATGAACAT AAAGTAACCA AAGCTCGTTT AACTGACAAA
    CATCAATCTA TTGAAGAAGC AAAGTCTGTT GCAATGTGTG AAATGGAAAA AAAGCTGAAG
    GAAGAGAGAG AAGCTCGAGA GAAGGCTGAA AATAGAGTTG TTCAGATTGA GAAACAGTGT
    TCAATGCTGG ATGTTGATCT GAAACAATCT CAGCAGAAGC TCGAACATTT GACTGAGAAT
    AAAGAAAGGA TGGAGGATGA AGTTAAGAAT CTATCCCTGC AGCTGGAGCA GGAATCAAAT
    AAGCGACTAT TGTTACAGAA TGAATTGAAG ACTCAAGCAT TTGAAGCAGA CAATTTAAAA
    GGTCTAGAAA AGCAGATGAA ACAGGAAATA AATACTTTAT TGGAAGCAAA GAGATTATTA
    GAATTTGAAT TAGCTCAGCT TACAAAACAG TATAGAGGAA ATGAAGGACA GATGCGGGAG
    CTACAAGATC AGCTTGAAGC TGAGCAGTAT TTCTCGACAC TTTATAAAAC CCAGGTAAAG
    GAACTTAAGG AAGAAATTGA AGAAAAAAAC AGAGAAAATT TAAAGAAAAT ACAAGAGCTA
    CAAAATGAAA AAGAAACTCT TGCTACTCAG TTGGATCTGG CAGAAACAAA AGCTGAGTCT
    GAGCAGTTGG CTCGAGGGCT TCTGGAAGAA CAGTATTTTG AATTAACCCA AGAAAGCAAG
    AAAGCTGCTT CCAGAAATAG GCAAGAGATT ACAGATAAAG ATCACACTGT TAGTCGGCTT
    GAAGAATCGA ACAGCATGTT AACCAAAGAT AATGAATTAT TAAGAAAAGA AAATGAAGAG
    CTAACTGATA AAATGAAGAG GGCAGAGGAA GAATATAAAT TGAAGAAGGA GGAGGAGATC
    AATAATCTTA AGGCTGCCTT TGAAAAGAAT ATCAATACAG AGAGAACCCT TAAAACGCAG
    GCTGTTAACA AGTTGGCAGA AATAATGAAT CGGAAAGATT TTAAAATTGA TAGAAAGAAA
    GCTAACACAC AGGATTTGAG AAAGAAAGAA AAGGAAAATC GAAAGCTCCA ACTGGAACTC
    AACCAAGAAA GAGAGAAATT CAACCAGATG GTGGTGAAGC ATCAGAAGGA ACTGAATGAC
    ATGCAAGCGC AATTGGTAGA AGAATGTACG CATAAGAATG AGCTTCAGAT GCAATTAGCT
    AGCAAAGAGA GTGATATTGA GCAACTGCGT GCCAAACTTT TGGACCTTTC CGATTCTACA
    AGTATTGCTA GTTTTCCTAG TGCTGATGAA ACTGATGGAA ACCTTCCAGA GTCAAGAATT
    GAAGGTTGGC TTTCAATACC AAATAGAGGA AATATCAAAC GATATGGCTG GAAGAAACAG
    TATGTTGTGG TAAGCAGCAA AAAAATTTTG TTCTATAATG ATGAACAAGA TAAGGAACAA
    TCCAATCCAT CCATGGTATT GGACATAGAT AAATTGTTTC ATGTTCGACC TGTAACCCAA
    GGAGATGTTT ACAGAGCTGA AACTGAAGAA ATTCCTAAAA TATTCCAGAT ACTATATGCA
    AATGAAGGTG AATGTAGAAA AGATGTAGAG ATGGAACCAG TACAACAAGC TGAAAAGACT
    AATTTTCAAA ACCACAAAGG CCATGAGTTC ATTCCTACAC TCTACCACTT CCCTGCCAAC
    TGTGAGGCCT GTGCCAAACC TCTCTGGCAT GTTTTTAAGC CACCTCCTGC CCTAGAATGT
    CGAAGATGCC ATGTTAAGTG CCACAGAGAT CACTTAGATA AGAAAGAGGA TTTAATTTCT
    CCATGTAAAG TAAGTTACGA TGTAACATCA GCAAGAGATA TGCTGCTGTT AGCATGTTCT
    CAGGATGAAC AAAAAAAATG GGTAACACAT TTGGTAAAGA AGATCCCTAA GAATCCACCA
    TCTGGTTTTG TTCGTGCATC GCCTCGAACG CTTTCTACAA GATCTACTGC AAATCAGTCT
    TTCCGAAAGG TGGTCAAAAA TACATCTGGA AAAACTAGCA GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.