ASH1L cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ASH1L
Entrez Gene ID	480128
Full Name	ASH1 like histone lysine methyltransferase
General protein information	Preferred Names ASH1 like histone lysine methyltransferase Names histone-lysine N-methyltransferase ASH1L
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014115336.2	XP_013970811.1	histone-lysine N-methyltransferase ASH1L
XM_537251.6	XP_537251.2	histone-lysine N-methyltransferase ASH1L
XM_014115338.2	XP_013970813.1	histone-lysine N-methyltransferase ASH1L
XM_537251.5	XP_537251.2	histone-lysine N-methyltransferase ASH1L
XM_014115337.1	XP_013970812.1	histone-lysine N-methyltransferase ASH1L
XM_014115338.1	XP_013970813.1	histone-lysine N-methyltransferase ASH1L
XM_014115336.1	XP_013970811.1	histone-lysine N-methyltransferase ASH1L
XM_014115337.2	XP_013970812.1	histone-lysine N-methyltransferase ASH1L

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ASH1L	XP_003949641.1
Bos taurus (cattle)	ASH1L	NP_001179672.1
Canis lupus familiaris (dog)	ASH1L	XP_537251.2
Rattus norvegicus (Norway rat)	Ash1l	NP_001101159.1
Mus musculus (house mouse)	Ash1l	NP_619620.3
Gallus gallus (chicken)	ASH1L	XP_004948339.1
Homo sapiens (human)	ASH1L	NP_060959.2

Gene Ontology

The following ASH1L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ASH1L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe09533	XM_014115337.2 Latest version!	Canis lupus familiaris ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCe09533	XM_014115336.2 Latest version!	Canis lupus familiaris ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCe09533	XM_537251.6 Latest version!	Canis lupus familiaris ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCe09533	XM_014115338.2 Latest version!	Canis lupus familiaris ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OCe09533	XM_537251.5	Canis lupus familiaris ash1 (absent, small, or homeotic)-like (Drosophila) (ASH1L), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCe09533	XM_014115338.1	Canis lupus familiaris ash1 (absent, small, or homeotic)-like (Drosophila) (ASH1L), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCe09533	XM_014115336.1	Canis lupus familiaris ash1 (absent, small, or homeotic)-like (Drosophila) (ASH1L), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCe09533	XM_014115337.1	Canis lupus familiaris ash1 (absent, small, or homeotic)-like (Drosophila) (ASH1L), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ASH1L ( XM_014115336.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013970811.1 Canis lupus familiaris ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X1, mRNA.

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CloneID	OCe09533
Clone ID Related Accession (Same CDS sequence)	XM_014115336.2 , XM_537251.6 , XM_014115338.2 , XM_537251.5 , XM_014115337.1 , XM_014115338.1 , XM_014115336.1 , XM_014115337.2
Accession Version	XM_014115336.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8898bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	histone-lysine N-methyltransferase ASH1L
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014115336.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCTA GAAATACTGC TATGTTAGGA TTGGGTTCTG ACTCGGAAGG ATTTTCAAGG 
AAGAGTCCCT CTGCCCTTGG TACCGGCACA GTGGTCAGCA AGAGAGAAGG AGAGCTAGAG 
AGCAACACGA AAGAGGAAGA GGACCTTCGC AAGAGGAATT GGGACAGAAA CAGCGAAGCA 
GGGAAGGAGG ATGGTTTGAC TGATGCACAG CAACAGTTTT CAGTGAAAGA AACAAACTTT 
TCAGAGGGAA ACTTAAAATT GAAAATTGGC CTTCAGGCTA AGAGAACTAA AAAACCTCCA 
AAAAACTTGG AGAACTATGT ATGTCGACCT GCCATAAAAA CAACTATCAA GCACCCAAGG 
AAAGCACTTA AAGGTGGGAA GATGACAGAG GAGAAGGATG AGCACTGTCC TTCAAGACGG 
GACCCTTCAA AGTTGTACAA GAAAACAGGT GATGTTGCAG CCGTTGAATG CCAATCTGAA 
GAGAGCATCC ATCTTCATTC ACAGGGAGAA AACAGTCCTT TGTCTAAGAA GCTTTCTCCA 
GGACACTCAG AGATGACAGA TTACATGAAT GCAGCATCAT CTACTCTTGT TGGTAGCCGG 
GATCCTGATT TAAAGGATAG AGCATTACTT AATGGAGGAA CAAGTGTAAC AGAAAAGTTG 
GCACAGCTTA TTGCAACCTG TCCTCCCTCT AAGTCTTCCA AGACTAAACC GAAGAAGTTA 
GGAAGTGGCA CCAGCGCAGG ATTGGTTAGT AAGGATTTGA TCAGGAAAGC AGGTGTTGGC 
TCTGTAGCTG GAATAATACA TAAAGATTTA ATTAAAAAAC CAGCTATCAG CACAGCAGTT 
GGATTGGTAA CTAAAGATCC TGGGAAAAAG CCAGTGTTTA ATGCAACAGT AGGATTGGTC 
AATAAGGACT CTGTGAAAAA ACTAGGAGCT GGCACTACAG CGGTATTCAT TAATAAAGAC 
TTAGGCAGAA AGCCAGGGAC TGTCACTACA GTAGGACTGC TGAGCAAGGA TTCAGGAAAG 
AAGCTAGGAA TTGGTATTGT TCCAGGTTTA GTGAATAAGG AATCTGGAAA GAAGTTAGGA 
CTCGGCACTG TGGTCGGACT GGTTAATAAA GACTTGGGCA AGAAGTTGAG TTCTACTGTC 
GGTCTAGTGG CCAAGGACTG TGCAAAGAAG ATTGTAGCCA ATTCAACAAT GGGATTAGTT 
ACTAAAGACA TGGGAAAGAA ACTAGTGAGT TGTTCTATGG CAGGATTGGT CAATAAAGAT 
GCCATAAACC TTAAAGCCGA AGCACTGCTC CCCACTCAGG AACCACTTAA GGCTTCTTGT 
AGTACAAACA TCAGTAGTCA TGAAAATCAG GAACTTTCTG AATCCCTGAA AGATAGTACC 
ACCAGCAAAA CTTTTGAGAA GAATGTTATA CGGCAGAGTA AAGAAAGCAT ATTGGAAAAG 
TTCTCAGTTC GAAAAGAAAT CATTAATTTG GAGAAAGAAA TGTTTAACGA GGGAACATGC 
ATTCAGCAAG ACAGTTTCTC ATCCAATGAC AGGGGGCCTT ATGAAACCTC AAAGCATGAA 
AAGCAACCTC CCGTGTATTG CACTTCTCCA GACTTTCAAA TGGGAGCTGC TTCTGATGCA 
TCTATGGCAA AATCCCCATT TAGTGCAGTA GGAGAAAGCA ATCTCCCTTC CCCATCACCT 
ACTGTATCTG TTAATCCTTT AACCAGAAGT CCCCCTGAAA CTTCTTCACA GTTGGCTCCT 
AATCCGTTAC TTTTAAGTCC TACCACAGAA CTAATGGAAG ACATTTCTGA ATCCGTTGGG 
AAGAACCAGT TTACTTCTGA AAGTACCCAC TTGAACATTG GTCATAGGTC TGTGGGTCAC 
AGCATGAATA TTGAATGTAA AGGGATTGAT AAAGAGCTAA CTGATTCAAA AACTGCACAT 
ATAGATATTC CAAGAATAAG CTCTTCCCTG GGAAAAAAGC CAAGTTTGAC TTCTGAATCC 
AGTATTCACA CAATTACCCC TTCAGTTGTT AACTTCACTA GTTTATTTAG TAACAAGCCG 
TTTCTAAAAC TTGGTGCAGT ATCTGCTTCT GACAAACACT GCCAGGTTGC TGAAAGCCTA 
AGCTCTAGTT TGCAGTCCAA ACCATTAAAA AAAAGGAAAG GAAGAAAAGC TCGGTGGACT 
AAAGTGGTGG CAAGAAGCAC ATGCCGGTCT CCAAAAGGGC TAGATTTAGA AAGATCAGAG 
CTTTTTAAAA GTGTTTCATG TAGCTCACTA TCAAATAGTA ATTCTGAGCC AGCCAAGTTC 
ATGAAAAACA TTGGACCATC TTCATTTGTA GATCATGACT TCCTTAAACG CCGATTGCCA 
AAGTTGAGCA AGTCCACAAC TCCATCTCTT GCTCTCTTAA CTGATAGTGA AAAACCATCT 
CACAAGGCTT TTGCTACTCA CAAATTATCC TCCAGTATGT GTGTCTCTAG TGACCTTTTG 
TCTGATATTT ATAAGCCCAA AAGAGGAAGA CCTAAAGCTA AAGAGATGCC TCAACTGGAA 
GGTCCACCTA AAAGGACTTT AAAAATCCCT GCCTCTAAAG TTTTTTCTTT GCAGTCTAAG 
GAAGAACAAG AACCCCCAAT TTTACAGCCA GAAATTGAAA TTCCTTCCTT CAAACAAAGT 
CTTTCTGTAT CTCCTTTTCC AAAAAAGCGA GGCAGACCTA AGAGGCAGTT GAGGTCACCG 
GTCAAGATGA AGCCACCTGT ACTATCAGTG GCTCCATTTG TTGCCACGGA GAGTCCAAGC 
AAGCTGGAGT CTGAAAGTGA TAACCATCGA AGCAGCAGTG ATTTCTTTGA GAGTGAGGAT 
CAGCTTCAGG ACCCAGATGA TCTGGACGAC AGTCATAGGC CGGCTGTCTG CAGCATGAGT 
GACCTTGAGA TGGAACCAGA TAAAAAAATT ACAAAGAGAA ACAATGGACA ATTAATGAAA 
ACAATTATCC GCAAAATAAA TAAAATGAAG ACTTTAAAAA GGAAGAAACT GTTGAATCAG 
ATTCTTTCAA GCTCTGTAGA ATCAAGTAAT AAAGGGAAAG TGCAATCCAA ACTCCATAAT 
ACAGTGTCAA GTCTTGCTGC CACGTTTGGC TCTAAATTGG GCCAACAGAT AAATGTCAGC 
AAGAAAGGAA CCATTTACAT AGGAAAGAGA AGGGGTCGAA AACCAAAAAC TGTCTTAAAT 
GGCATTCTTT CTGGTAGCCC TACCAGCCTT GCTGTCCTTG AGCAAACTGC TCAACAGGCT 
GCCGGCTCAG CATTGGGACA GATACTTCCC CCTTTACTGC CTTCATCTGC TAGTAGCTCA 
GAGATTCTCC CATCGCCTGT TTGCTCTCAG TCTTCTGGGA CTAGTGGTGG TCAGAGCCCT 
GTAAGTAGTG ATGCAGGCTT TGTTGAACCC AGCTCAGTGC CATATTTGCA TTTACACTCC 
AGACAGGGCA GTATGATTCA GACTCTTGCG ATGAAAAAGG CCTCAAAGGG GAGGAGGCGA 
CTTTCTCCTC CTACTTTGTT GCCAAATTCT CCTTCACACT TGAGTGAACT CACCTCTCTG 
AAAGAAGCTA CTCCTTCTCC CATTAGTGAG TCTCATAGTG ATGAGACCAT TCCCAGTGAT 
AGTGGAATTG GAACAGATAA TAACAGCACA TCGGACAGAG CAGAGAAATT TTGTGGACAG 
AAAAAGAGGA GGCACTCTTT TGAACATGTT TCTCTGATTC CCCCTGAAAC CTCTACAGTC 
CTGAGCAGTC TTAAAGAAAA ACATAAACAT AAATGTAAGC GCCGGAATCA CGATTACCTC 
AGCTATGACA AGATGAAAAG GCAGAAACGA AAACGGAAAA AGAAATACCC CCAGCTCCGT 
AGCAGACAGG ATCCAGACTT TATTGCGGAT CTGGAGGAAT TGATAAGTCG GCTGAGTGAA 
ATTCGAATCA CCCATCGAAG CCATCATTTT ATCCCCCGAG ACCTGCTGCC AACTATTTTT 
CGAATCAACT TTAATAGTTT CTATACACAT CCTTCTTTCC CCTTAGACCC CTTGCACTAC 
ATTCGAAAAC CTGACTTAAA AAAGAAGAGA GGGAGACCTC CTAAGATGAG GGAGGCAATG 
GCTGAGATGC CCTTCATGCA CAGCCTTAGT TTTCCTCTTT CTAGTACTGG ATTCTATCCC 
TCCTATGGCA TGCCTTACTC TCCCTCACCC CTTACAGCCA CTCCCCTAGG ATTAGGTTAC 
TATGGAAGGT ACCCTCCCAC TCTCTATCCT CCTCCTCCTT CACCTTCTTT CACCCCCCCA 
CTTCCTCCTC CTTCCTATAT GCATGCTGGC CATTTACTTC TCAATCCAAC CAAATACCAT 
AAGAAAAAGC ATAAGCTACT TCGCCAGGAG GCCTTTCTAA CAACCAGCAG GACTCCCCTC 
CTTTCCATGA GTACCTACCC TAGTGTTCCT CCTGAGATGG CCTATGGTTG GATGGTTGAG 
CACAAACATA GGCATCGTCA CAAGCACAGA GAACACCGTT CTTCTGAGCA ACCCCAGGTT 
TCCATGGATG CTGGCTCTTC CAGATCTGTT TTGGAGTCTT TGAAGCGCTA TCGATTTGGA 
AAGGAAACTG TTGGAGAGCG ATATAAACAT AAGGAAAAGC ACCGATGTCA TATGTCCTGC 
CCTCATCTCT CTCCTTCGAA AAGCTTAATA AATAGAGAAG AACAATGGGT CCACCGAGAG 
CCTTCAGAAT CTAGTCCATT GGCTTTGGGA TTGCAAACAC CTTTACAGAT TGACTGTTCC 
GAAAGTTCTC CAAGTTTATC TCTTGGAGGA TTCACTCCCA ACTCTGATCC GGCCAGCAGT 
GATGAACATA CAAACCTTTT CACAAGTGCA ATAGTGCAAG GCAGCTGCAG AGTTTCAAAC 
CCTAACTCCA GTGGCCGGAA GAAATTAACT GATAGCCCTG GACTGTTTTC TGCACAGGAC 
ACTTCACTAA TTCGGCCTCA CAGAAAGGAG CCACTGCCTT CCAGCGAAAG GGCAGTACAG 
ACCATGACAG GTTCCCAGCC AACCTCAGAT AAGTCTTCCC AGCGACCATC AGAGAGCACA 
AACTGTAGCC CTACCCGGAA GAGGTCTTCA TCTGAGAGTA CTTCTTCAAC AGTAAACGGA 
CTTCCTTCTC GAAGTCCAAG ATTGCTTGCC CCTGGGGATG ACTCTGTGGA TAGTCTGCTG 
CAGCGGATGG TACAACATGA GGACCAAGAG CCCCTGGAGA AGAATCTAGA TGCCGTGATT 
GCATCTGCCT CGGGGCCACC TTCTTCCAGT CCAGGCCACA GCCACAGCAA GGAACGAGCC 
CTGGGAAAAC CAGATAGCCT TCTAGTGCCC GCAGCCCCCA GTGACTCTTG TAGCAGCAGC 
ATCTCACTCC TGTCTGAGAA GTTGCCGAGC AGCTGCTCAC CCCACCATAT CAAGAGGAGT 
GTCGTGGAAG CCATGCAGCG CCAAGCTCGG AAAATGTGCA ATTATGACAA AATCTTGGCC 
ACAAAGAAAA ACCTGGACCA TGTCAATAAA ATATTAAAAG CCAAAAAGCT TCAAAGACAG 
GCCAGGACAG GGAATAACTT CGTGAAACGC AGACCAGGGC GACCTCGGAA ATGCCCCCTC 
CAGGCAGTGG TGTCAATGCA AGCATTCCAG GCTGCCCGAT TCGTCAGCCC CGATGCTAAT 
GAAGGTGAAG AAAGAGCAGC CCTGCCCCTC CGTCCAGACA CAGTTACGGA TGTCATCGAG 
GCTGTTGTGC AGAGCGTAAA CCTGAGTCCA GAACATAAGA AGGGGCTGAA GAGGAAAAGT 
TGGCTGTTGG AAGAACAGAC CAAGAAAAAG CAGAAGCCAT TCCCAGAGGA AGAACAAGAG 
CATCCCAAGA GCTTTACTGA AGCAGCAGTT GAGATTCCCA GTCCTCCTGA AACCCCAGCC 
AAGCCTTCCG AACCTGAAAG TACCTTGCAG CCTGTGCTTT CTCTCATCCC AAGGGAAAAG 
AAGGCCCCAC GTCCCCCAAA GAAGAAGTAT CAGAAAGCAG GGCTGTATTC TGACGTTTAC 
AAAACTACAG ACCCAAAGAG CCGATTGATC CAATTAAAGA AAGAAAAGTT GGAATACACT 
CCAGGAGAGC ATGAGTATGG ATTATTCCCA GCCCCTATTC ATGTTGGAAA GTATCTAAGG 
CAAAAGAGAA TTGACTTCCA GCTTCCCTAT GACATCCTTT GGCAGTGGAA ACATAATCAG 
CTATACAAAA AGCCAGATGT CCCACTATAT AAAAAAATCC GTTCAAACGT CTACGTGGAT 
GTCAAACCCC TTTCTGGTTA CGAGGCCACC ACCTGTAACT GTAAGAAGCC AGAGGATGAC 
ACCGGGAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCT 
AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGAGGCA TGAGTGGGTG 
CAGTGTCTAG AACGATTTCG AGCTGAGGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC 
CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGGGAGG TTGTCAGCGA ACAGGAGTTC 
AGGAACAGAA TGATTGAGCA GTATCATAAT CACAGTGACC ACTATTGCCT GAACCTGGAT 
AGTGGGATGG TGATTGACAG TTATCGGATG GGTAATGAGG CCCGATTCAT CAACCACAGC 
TGTGACCCAA ACTGTGAAAT GCAGAAATGG TCTGTCAATG GAGTATACCG GATTGGACTG 
TATGCTCTTA AAGATATGCC AGCTGGTACC GAGCTCACTT ATGATTACAA TTTCCATTCC 
TTTAATGTTG AGAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT 
GGGGGCAAGA GTCAGCGCGT GAACGGACTC GCCAGCAGCA AAAGCAGCCA GCCTGTGGCT 
ACCCATAAAA AGTCTGGACG GTCAAAAGAG AAGAGAAAGT CAAAGCACAA GCTGAAGAAA 
AGGAGAGGCC ACCTCTCCGA AGAACCCAGT GAAAACATCA ACACTCCAAC AAGATTGACC 
CCCCAATTAC AAATGAAGCC AATGTCTAAT CGAGAGAGGA ACTTCGTGTT AAAGCATCAT 
GTATTCTTGG TCCGAAACTG GGAGAAGATC CGACAGAAAC AAGAGGAAGT AAAGCATACC 
AGCGATAATC TCCACTCTGC ATCATTATAC ACCCGCTGGA ATGGCATCTG CCGAGATGAT 
GGGAACATCA AGTCTGACGT CTTCATGACC CAGTTCTCCG CCCTGCAGAC AGCACGATCA 
GTTCGAACAA GACGCCTGGC AGCAGCAGAG GAAAACATCG AGGTGGCACG GGCAGCCCGC 
CTCGCCCAAA TCTTCAAAGA GATCTGTGAT GGCATCATCT CCTACAAAGA TTCTTCCCGA 
CAAGCTTTGG CAGCACCCCT CTTGAACCTC CCCCCAAAGA AAAAGAATGC TGATTACTAT 
GAGAAGATCT CAGAGCCCCT GGACTTGGCC ACCATAGAGA GGCAGATCCT CACCGGCCAT 
TACAAGACCG TGGAGGCCTT TGACGCTGAC ATGCTCAAGG TGTTTCGGAA TGCTGAGAAG 
TACTACGGGC GTAAATCCCC AATTGGGAGA GATGTTTGCC GTCTACGGAA GACCTACTAC 
AACGCCAGGC ATGAGGCATC AGCCCAGATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG 
GCCGACAGCA GCGAAACATC TGTCTCTGAG AAGGAGAACG TGCATGAGAA GGATGACGAT 
GTGATCCGCT GTGTCTGTGG CCTCCACAAG GATGAGGGCC TCATGATCCA GTGTGACAAG 
TGCATGGTAT GGCAGCACTG TGACTGTATG GGGGTGAACT CAGACGTGGA GCATTACCTG 
TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC 
TATGCCCAGC CCGGCTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GTTGCTTCGT 
CAAGGTGACT GTGTGTACCT GATGCGAGAT AGCCGGCGCA CCCCTGATGG CCACCCAGTC 
CGCCAGTCCT ACCGACTGTT ATCACACATT AACCGAGATA AACTTGACAT CTTCCGCATT 
GAGAAGCTCT GGAAGAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA CTACTTCCGC 
CCCCACGAGA CCCACCACTC TCCGTCACGC CGCTTCTATC ACAATGAGCT ATTTAGGGTA 
CCACTCTATG AGATCATTCC CTTGGAGGCT GTGGTGGGGA CCTGCTGTGT ACTAGACCTT 
TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTATA CATCTGTGAC 
TATCGGCTTG ACAAGTCCGC ACACCTGTTT TACAAGATTC ACCGGAATCG CTATCCTGTC 
TGCACCAAAC CCTACGCCTT TGACCACTTC CCCAAGAAGC TCACTCCTAA AAGAGATTTC 
TCACCTCATT ATGTCCCAGA CAACTACAAG AGGAACGGAG GGCGGTCATC CTGGAAGTCT 
GAGCGCCCAA AGCCACCTCT AAAAGACCTG GGCCAGGAGG ATGACGCTCT GCCCTTGATT 
GAAGAGGTTC TGGCCAGCCA GGAGCAAGCC ACCAATGAGC TGCCCAGCCT GGAGGAGCCA 
GAACGGGAAG TGGCCCCTGT GGAAGTCAGC GAGGGTGAAA AGAAAGCAGA GGAAGGTGGT 
CCAGAATCTC AGGCTGCCTG TACCCCTGAG GAGCGACGGC ACAGCCAACG GGAACGGCTC 
AACCAGATCC TACTTGGCCT CCTGGAAAAA ATCCCTGGGA AAAATGCCAT CGATGTGACC 
TACTTGCTGG AGGAAGGGTC AGGCAGGAAG CTTCGAAGGC GCACTTTGTT CATCCCAGAG 
AACAGCTTTC GGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970811.1
CDS	309..9206
Translation	MDPRNTAMLGLGSDSEGFSRKSPSALGTGTVVSKREGELESNTKEEEDLRKRNWDRNSEAGKEDGLTDAQQQFSVKETNFSEGNLKLKIGLQAKRTKKPPKNLENYVCRPAIKTTIKHPRKALKGGKMTEEKDEHCPSRRDPSKLYKKTGDVAAVECQSEESIHLHSQGENSPLSKKLSPGHSEMTDYMNAASSTLVGSRDPDLKDRALLNGGTSVTEKLAQLIATCPPSKSSKTKPKKLGSGTSAGLVSKDLIRKAGVGSVAGIIHKDLIKKPAISTAVGLVTKDPGKKPVFNATVGLVNKDSVKKLGAGTTAVFINKDLGRKPGTVTTVGLLSKDSGKKLGIGIVPGLVNKESGKKLGLGTVVGLVNKDLGKKLSSTVGLVAKDCAKKIVANSTMGLVTKDMGKKLVSCSMAGLVNKDAINLKAEALLPTQEPLKASCSTNISSHENQELSESLKDSTTSKTFEKNVIRQSKESILEKFSVRKEIINLEKEMFNEGTCIQQDSFSSNDRGPYETSKHEKQPPVYCTSPDFQMGAASDASMAKSPFSAVGESNLPSPSPTVSVNPLTRSPPETSSQLAPNPLLLSPTTELMEDISESVGKNQFTSESTHLNIGHRSVGHSMNIECKGIDKELTDSKTAHIDIPRISSSLGKKPSLTSESSIHTITPSVVNFTSLFSNKPFLKLGAVSASDKHCQVAESLSSSLQSKPLKKRKGRKARWTKVVARSTCRSPKGLDLERSELFKSVSCSSLSNSNSEPAKFMKNIGPSSFVDHDFLKRRLPKLSKSTTPSLALLTDSEKPSHKAFATHKLSSSMCVSSDLLSDIYKPKRGRPKAKEMPQLEGPPKRTLKIPASKVFSLQSKEEQEPPILQPEIEIPSFKQSLSVSPFPKKRGRPKRQLRSPVKMKPPVLSVAPFVATESPSKLESESDNHRSSSDFFESEDQLQDPDDLDDSHRPAVCSMSDLEMEPDKKITKRNNGQLMKTIIRKINKMKTLKRKKLLNQILSSSVESSNKGKVQSKLHNTVSSLAATFGSKLGQQINVSKKGTIYIGKRRGRKPKTVLNGILSGSPTSLAVLEQTAQQAAGSALGQILPPLLPSSASSSEILPSPVCSQSSGTSGGQSPVSSDAGFVEPSSVPYLHLHSRQGSMIQTLAMKKASKGRRRLSPPTLLPNSPSHLSELTSLKEATPSPISESHSDETIPSDSGIGTDNNSTSDRAEKFCGQKKRRHSFEHVSLIPPETSTVLSSLKEKHKHKCKRRNHDYLSYDKMKRQKRKRKKKYPQLRSRQDPDFIADLEELISRLSEIRITHRSHHFIPRDLLPTIFRINFNSFYTHPSFPLDPLHYIRKPDLKKKRGRPPKMREAMAEMPFMHSLSFPLSSTGFYPSYGMPYSPSPLTATPLGLGYYGRYPPTLYPPPPSPSFTPPLPPPSYMHAGHLLLNPTKYHKKKHKLLRQEAFLTTSRTPLLSMSTYPSVPPEMAYGWMVEHKHRHRHKHREHRSSEQPQVSMDAGSSRSVLESLKRYRFGKETVGERYKHKEKHRCHMSCPHLSPSKSLINREEQWVHREPSESSPLALGLQTPLQIDCSESSPSLSLGGFTPNSDPASSDEHTNLFTSAIVQGSCRVSNPNSSGRKKLTDSPGLFSAQDTSLIRPHRKEPLPSSERAVQTMTGSQPTSDKSSQRPSESTNCSPTRKRSSSESTSSTVNGLPSRSPRLLAPGDDSVDSLLQRMVQHEDQEPLEKNLDAVIASASGPPSSSPGHSHSKERALGKPDSLLVPAAPSDSCSSSISLLSEKLPSSCSPHHIKRSVVEAMQRQARKMCNYDKILATKKNLDHVNKILKAKKLQRQARTGNNFVKRRPGRPRKCPLQAVVSMQAFQAARFVSPDANEGEERAALPLRPDTVTDVIEAVVQSVNLSPEHKKGLKRKSWLLEEQTKKKQKPFPEEEQEHPKSFTEAAVEIPSPPETPAKPSEPESTLQPVLSLIPREKKAPRPPKKKYQKAGLYSDVYKTTDPKSRLIQLKKEKLEYTPGEHEYGLFPAPIHVGKYLRQKRIDFQLPYDILWQWKHNQLYKKPDVPLYKKIRSNVYVDVKPLSGYEATTCNCKKPEDDTGKGCVDDCLNRMIFAECSPNTCPCGEQCCNQRIQRHEWVQCLERFRAEEKGWGIRTKEPLKAGQFIIEYLGEVVSEQEFRNRMIEQYHNHSDHYCLNLDSGMVIDSYRMGNEARFINHSCDPNCEMQKWSVNGVYRIGLYALKDMPAGTELTYDYNFHSFNVEKQQLCKCGFEKCRGIIGGKSQRVNGLASSKSSQPVATHKKSGRSKEKRKSKHKLKKRRGHLSEEPSENINTPTRLTPQLQMKPMSNRERNFVLKHHVFLVRNWEKIRQKQEEVKHTSDNLHSASLYTRWNGICRDDGNIKSDVFMTQFSALQTARSVRTRRLAAAEENIEVARAARLAQIFKEICDGIISYKDSSRQALAAPLLNLPPKKKNADYYEKISEPLDLATIERQILTGHYKTVEAFDADMLKVFRNAEKYYGRKSPIGRDVCRLRKTYYNARHEASAQIDEIVGETASEADSSETSVSEKENVHEKDDDVIRCVCGLHKDEGLMIQCDKCMVWQHCDCMGVNSDVEHYLCEQCDPRPVDREVPMIPRPHYAQPGCVYFICLLRDDLLLRQGDCVYLMRDSRRTPDGHPVRQSYRLLSHINRDKLDIFRIEKLWKNEKEERFAFGHHYFRPHETHHSPSRRFYHNELFRVPLYEIIPLEAVVGTCCVLDLYTYCKGRPKGVKEQDVYICDYRLDKSAHLFYKIHRNRYPVCTKPYAFDHFPKKLTPKRDFSPHYVPDNYKRNGGRSSWKSERPKPPLKDLGQEDDALPLIEEVLASQEQATNELPSLEEPEREVAPVEVSEGEKKAEEGGPESQAACTPEERRHSQRERLNQILLGLLEKIPGKNAIDVTYLLEEGSGRKLRRRTLFIPENSFRK

Target ORF information:

RefSeq Version	XM_014115336.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115336.2

ORF Insert Sequence:

ATGGACCCTA GAAATACTGC TATGTTAGGA TTGGGTTCTG ACTCGGAAGG ATTTTCAAGG 
AAGAGTCCCT CTGCCCTTGG TACCGGCACA GTGGTCAGCA AGAGAGAAGG AGAGCTAGAG 
AGCAACACGA AAGAGGAAGA GGACCTTCGC AAGAGGAATT GGGACAGAAA CAGCGAAGCA 
GGGAAGGAGG ATGGTTTGAC TGATGCACAG CAACAGTTTT CAGTGAAAGA AACAAACTTT 
TCAGAGGGAA ACTTAAAATT GAAAATTGGC CTTCAGGCTA AGAGAACTAA AAAACCTCCA 
AAAAACTTGG AGAACTATGT ATGTCGACCT GCCATAAAAA CAACTATCAA GCACCCAAGG 
AAAGCACTTA AAGGTGGGAA GATGACAGAG GAGAAGGATG AGCACTGTCC TTCAAGACGG 
GACCCTTCAA AGTTGTACAA GAAAACAGGT GATGTTGCAG CCGTTGAATG CCAATCTGAA 
GAGAGCATCC ATCTTCATTC ACAGGGAGAA AACAGTCCTT TGTCTAAGAA GCTTTCTCCA 
GGACACTCAG AGATGACAGA TTACATGAAT GCAGCATCAT CTACTCTTGT TGGTAGCCGG 
GATCCTGATT TAAAGGATAG AGCATTACTT AATGGAGGAA CAAGTGTAAC AGAAAAGTTG 
GCACAGCTTA TTGCAACCTG TCCTCCCTCT AAGTCTTCCA AGACTAAACC GAAGAAGTTA 
GGAAGTGGCA CCAGCGCAGG ATTGGTTAGT AAGGATTTGA TCAGGAAAGC AGGTGTTGGC 
TCTGTAGCTG GAATAATACA TAAAGATTTA ATTAAAAAAC CAGCTATCAG CACAGCAGTT 
GGATTGGTAA CTAAAGATCC TGGGAAAAAG CCAGTGTTTA ATGCAACAGT AGGATTGGTC 
AATAAGGACT CTGTGAAAAA ACTAGGAGCT GGCACTACAG CGGTATTCAT TAATAAAGAC 
TTAGGCAGAA AGCCAGGGAC TGTCACTACA GTAGGACTGC TGAGCAAGGA TTCAGGAAAG 
AAGCTAGGAA TTGGTATTGT TCCAGGTTTA GTGAATAAGG AATCTGGAAA GAAGTTAGGA 
CTCGGCACTG TGGTCGGACT GGTTAATAAA GACTTGGGCA AGAAGTTGAG TTCTACTGTC 
GGTCTAGTGG CCAAGGACTG TGCAAAGAAG ATTGTAGCCA ATTCAACAAT GGGATTAGTT 
ACTAAAGACA TGGGAAAGAA ACTAGTGAGT TGTTCTATGG CAGGATTGGT CAATAAAGAT 
GCCATAAACC TTAAAGCCGA AGCACTGCTC CCCACTCAGG AACCACTTAA GGCTTCTTGT 
AGTACAAACA TCAGTAGTCA TGAAAATCAG GAACTTTCTG AATCCCTGAA AGATAGTACC 
ACCAGCAAAA CTTTTGAGAA GAATGTTATA CGGCAGAGTA AAGAAAGCAT ATTGGAAAAG 
TTCTCAGTTC GAAAAGAAAT CATTAATTTG GAGAAAGAAA TGTTTAACGA GGGAACATGC 
ATTCAGCAAG ACAGTTTCTC ATCCAATGAC AGGGGGCCTT ATGAAACCTC AAAGCATGAA 
AAGCAACCTC CCGTGTATTG CACTTCTCCA GACTTTCAAA TGGGAGCTGC TTCTGATGCA 
TCTATGGCAA AATCCCCATT TAGTGCAGTA GGAGAAAGCA ATCTCCCTTC CCCATCACCT 
ACTGTATCTG TTAATCCTTT AACCAGAAGT CCCCCTGAAA CTTCTTCACA GTTGGCTCCT 
AATCCGTTAC TTTTAAGTCC TACCACAGAA CTAATGGAAG ACATTTCTGA ATCCGTTGGG 
AAGAACCAGT TTACTTCTGA AAGTACCCAC TTGAACATTG GTCATAGGTC TGTGGGTCAC 
AGCATGAATA TTGAATGTAA AGGGATTGAT AAAGAGCTAA CTGATTCAAA AACTGCACAT 
ATAGATATTC CAAGAATAAG CTCTTCCCTG GGAAAAAAGC CAAGTTTGAC TTCTGAATCC 
AGTATTCACA CAATTACCCC TTCAGTTGTT AACTTCACTA GTTTATTTAG TAACAAGCCG 
TTTCTAAAAC TTGGTGCAGT ATCTGCTTCT GACAAACACT GCCAGGTTGC TGAAAGCCTA 
AGCTCTAGTT TGCAGTCCAA ACCATTAAAA AAAAGGAAAG GAAGAAAAGC TCGGTGGACT 
AAAGTGGTGG CAAGAAGCAC ATGCCGGTCT CCAAAAGGGC TAGATTTAGA AAGATCAGAG 
CTTTTTAAAA GTGTTTCATG TAGCTCACTA TCAAATAGTA ATTCTGAGCC AGCCAAGTTC 
ATGAAAAACA TTGGACCATC TTCATTTGTA GATCATGACT TCCTTAAACG CCGATTGCCA 
AAGTTGAGCA AGTCCACAAC TCCATCTCTT GCTCTCTTAA CTGATAGTGA AAAACCATCT 
CACAAGGCTT TTGCTACTCA CAAATTATCC TCCAGTATGT GTGTCTCTAG TGACCTTTTG 
TCTGATATTT ATAAGCCCAA AAGAGGAAGA CCTAAAGCTA AAGAGATGCC TCAACTGGAA 
GGTCCACCTA AAAGGACTTT AAAAATCCCT GCCTCTAAAG TTTTTTCTTT GCAGTCTAAG 
GAAGAACAAG AACCCCCAAT TTTACAGCCA GAAATTGAAA TTCCTTCCTT CAAACAAAGT 
CTTTCTGTAT CTCCTTTTCC AAAAAAGCGA GGCAGACCTA AGAGGCAGTT GAGGTCACCG 
GTCAAGATGA AGCCACCTGT ACTATCAGTG GCTCCATTTG TTGCCACGGA GAGTCCAAGC 
AAGCTGGAGT CTGAAAGTGA TAACCATCGA AGCAGCAGTG ATTTCTTTGA GAGTGAGGAT 
CAGCTTCAGG ACCCAGATGA TCTGGACGAC AGTCATAGGC CGGCTGTCTG CAGCATGAGT 
GACCTTGAGA TGGAACCAGA TAAAAAAATT ACAAAGAGAA ACAATGGACA ATTAATGAAA 
ACAATTATCC GCAAAATAAA TAAAATGAAG ACTTTAAAAA GGAAGAAACT GTTGAATCAG 
ATTCTTTCAA GCTCTGTAGA ATCAAGTAAT AAAGGGAAAG TGCAATCCAA ACTCCATAAT 
ACAGTGTCAA GTCTTGCTGC CACGTTTGGC TCTAAATTGG GCCAACAGAT AAATGTCAGC 
AAGAAAGGAA CCATTTACAT AGGAAAGAGA AGGGGTCGAA AACCAAAAAC TGTCTTAAAT 
GGCATTCTTT CTGGTAGCCC TACCAGCCTT GCTGTCCTTG AGCAAACTGC TCAACAGGCT 
GCCGGCTCAG CATTGGGACA GATACTTCCC CCTTTACTGC CTTCATCTGC TAGTAGCTCA 
GAGATTCTCC CATCGCCTGT TTGCTCTCAG TCTTCTGGGA CTAGTGGTGG TCAGAGCCCT 
GTAAGTAGTG ATGCAGGCTT TGTTGAACCC AGCTCAGTGC CATATTTGCA TTTACACTCC 
AGACAGGGCA GTATGATTCA GACTCTTGCG ATGAAAAAGG CCTCAAAGGG GAGGAGGCGA 
CTTTCTCCTC CTACTTTGTT GCCAAATTCT CCTTCACACT TGAGTGAACT CACCTCTCTG 
AAAGAAGCTA CTCCTTCTCC CATTAGTGAG TCTCATAGTG ATGAGACCAT TCCCAGTGAT 
AGTGGAATTG GAACAGATAA TAACAGCACA TCGGACAGAG CAGAGAAATT TTGTGGACAG 
AAAAAGAGGA GGCACTCTTT TGAACATGTT TCTCTGATTC CCCCTGAAAC CTCTACAGTC 
CTGAGCAGTC TTAAAGAAAA ACATAAACAT AAATGTAAGC GCCGGAATCA CGATTACCTC 
AGCTATGACA AGATGAAAAG GCAGAAACGA AAACGGAAAA AGAAATACCC CCAGCTCCGT 
AGCAGACAGG ATCCAGACTT TATTGCGGAT CTGGAGGAAT TGATAAGTCG GCTGAGTGAA 
ATTCGAATCA CCCATCGAAG CCATCATTTT ATCCCCCGAG ACCTGCTGCC AACTATTTTT 
CGAATCAACT TTAATAGTTT CTATACACAT CCTTCTTTCC CCTTAGACCC CTTGCACTAC 
ATTCGAAAAC CTGACTTAAA AAAGAAGAGA GGGAGACCTC CTAAGATGAG GGAGGCAATG 
GCTGAGATGC CCTTCATGCA CAGCCTTAGT TTTCCTCTTT CTAGTACTGG ATTCTATCCC 
TCCTATGGCA TGCCTTACTC TCCCTCACCC CTTACAGCCA CTCCCCTAGG ATTAGGTTAC 
TATGGAAGGT ACCCTCCCAC TCTCTATCCT CCTCCTCCTT CACCTTCTTT CACCCCCCCA 
CTTCCTCCTC CTTCCTATAT GCATGCTGGC CATTTACTTC TCAATCCAAC CAAATACCAT 
AAGAAAAAGC ATAAGCTACT TCGCCAGGAG GCCTTTCTAA CAACCAGCAG GACTCCCCTC 
CTTTCCATGA GTACCTACCC TAGTGTTCCT CCTGAGATGG CCTATGGTTG GATGGTTGAG 
CACAAACATA GGCATCGTCA CAAGCACAGA GAACACCGTT CTTCTGAGCA ACCCCAGGTT 
TCCATGGATG CTGGCTCTTC CAGATCTGTT TTGGAGTCTT TGAAGCGCTA TCGATTTGGA 
AAGGAAACTG TTGGAGAGCG ATATAAACAT AAGGAAAAGC ACCGATGTCA TATGTCCTGC 
CCTCATCTCT CTCCTTCGAA AAGCTTAATA AATAGAGAAG AACAATGGGT CCACCGAGAG 
CCTTCAGAAT CTAGTCCATT GGCTTTGGGA TTGCAAACAC CTTTACAGAT TGACTGTTCC 
GAAAGTTCTC CAAGTTTATC TCTTGGAGGA TTCACTCCCA ACTCTGATCC GGCCAGCAGT 
GATGAACATA CAAACCTTTT CACAAGTGCA ATAGTGCAAG GCAGCTGCAG AGTTTCAAAC 
CCTAACTCCA GTGGCCGGAA GAAATTAACT GATAGCCCTG GACTGTTTTC TGCACAGGAC 
ACTTCACTAA TTCGGCCTCA CAGAAAGGAG CCACTGCCTT CCAGCGAAAG GGCAGTACAG 
ACCATGACAG GTTCCCAGCC AACCTCAGAT AAGTCTTCCC AGCGACCATC AGAGAGCACA 
AACTGTAGCC CTACCCGGAA GAGGTCTTCA TCTGAGAGTA CTTCTTCAAC AGTAAACGGA 
CTTCCTTCTC GAAGTCCAAG ATTGCTTGCC CCTGGGGATG ACTCTGTGGA TAGTCTGCTG 
CAGCGGATGG TACAACATGA GGACCAAGAG CCCCTGGAGA AGAATCTAGA TGCCGTGATT 
GCATCTGCCT CGGGGCCACC TTCTTCCAGT CCAGGCCACA GCCACAGCAA GGAACGAGCC 
CTGGGAAAAC CAGATAGCCT TCTAGTGCCC GCAGCCCCCA GTGACTCTTG TAGCAGCAGC 
ATCTCACTCC TGTCTGAGAA GTTGCCGAGC AGCTGCTCAC CCCACCATAT CAAGAGGAGT 
GTCGTGGAAG CCATGCAGCG CCAAGCTCGG AAAATGTGCA ATTATGACAA AATCTTGGCC 
ACAAAGAAAA ACCTGGACCA TGTCAATAAA ATATTAAAAG CCAAAAAGCT TCAAAGACAG 
GCCAGGACAG GGAATAACTT CGTGAAACGC AGACCAGGGC GACCTCGGAA ATGCCCCCTC 
CAGGCAGTGG TGTCAATGCA AGCATTCCAG GCTGCCCGAT TCGTCAGCCC CGATGCTAAT 
GAAGGTGAAG AAAGAGCAGC CCTGCCCCTC CGTCCAGACA CAGTTACGGA TGTCATCGAG 
GCTGTTGTGC AGAGCGTAAA CCTGAGTCCA GAACATAAGA AGGGGCTGAA GAGGAAAAGT 
TGGCTGTTGG AAGAACAGAC CAAGAAAAAG CAGAAGCCAT TCCCAGAGGA AGAACAAGAG 
CATCCCAAGA GCTTTACTGA AGCAGCAGTT GAGATTCCCA GTCCTCCTGA AACCCCAGCC 
AAGCCTTCCG AACCTGAAAG TACCTTGCAG CCTGTGCTTT CTCTCATCCC AAGGGAAAAG 
AAGGCCCCAC GTCCCCCAAA GAAGAAGTAT CAGAAAGCAG GGCTGTATTC TGACGTTTAC 
AAAACTACAG ACCCAAAGAG CCGATTGATC CAATTAAAGA AAGAAAAGTT GGAATACACT 
CCAGGAGAGC ATGAGTATGG ATTATTCCCA GCCCCTATTC ATGTTGGAAA GTATCTAAGG 
CAAAAGAGAA TTGACTTCCA GCTTCCCTAT GACATCCTTT GGCAGTGGAA ACATAATCAG 
CTATACAAAA AGCCAGATGT CCCACTATAT AAAAAAATCC GTTCAAACGT CTACGTGGAT 
GTCAAACCCC TTTCTGGTTA CGAGGCCACC ACCTGTAACT GTAAGAAGCC AGAGGATGAC 
ACCGGGAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCT 
AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGAGGCA TGAGTGGGTG 
CAGTGTCTAG AACGATTTCG AGCTGAGGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC 
CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGGGAGG TTGTCAGCGA ACAGGAGTTC 
AGGAACAGAA TGATTGAGCA GTATCATAAT CACAGTGACC ACTATTGCCT GAACCTGGAT 
AGTGGGATGG TGATTGACAG TTATCGGATG GGTAATGAGG CCCGATTCAT CAACCACAGC 
TGTGACCCAA ACTGTGAAAT GCAGAAATGG TCTGTCAATG GAGTATACCG GATTGGACTG 
TATGCTCTTA AAGATATGCC AGCTGGTACC GAGCTCACTT ATGATTACAA TTTCCATTCC 
TTTAATGTTG AGAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT 
GGGGGCAAGA GTCAGCGCGT GAACGGACTC GCCAGCAGCA AAAGCAGCCA GCCTGTGGCT 
ACCCATAAAA AGTCTGGACG GTCAAAAGAG AAGAGAAAGT CAAAGCACAA GCTGAAGAAA 
AGGAGAGGCC ACCTCTCCGA AGAACCCAGT GAAAACATCA ACACTCCAAC AAGATTGACC 
CCCCAATTAC AAATGAAGCC AATGTCTAAT CGAGAGAGGA ACTTCGTGTT AAAGCATCAT 
GTATTCTTGG TCCGAAACTG GGAGAAGATC CGACAGAAAC AAGAGGAAGT AAAGCATACC 
AGCGATAATC TCCACTCTGC ATCATTATAC ACCCGCTGGA ATGGCATCTG CCGAGATGAT 
GGGAACATCA AGTCTGACGT CTTCATGACC CAGTTCTCCG CCCTGCAGAC AGCACGATCA 
GTTCGAACAA GACGCCTGGC AGCAGCAGAG GAAAACATCG AGGTGGCACG GGCAGCCCGC 
CTCGCCCAAA TCTTCAAAGA GATCTGTGAT GGCATCATCT CCTACAAAGA TTCTTCCCGA 
CAAGCTTTGG CAGCACCCCT CTTGAACCTC CCCCCAAAGA AAAAGAATGC TGATTACTAT 
GAGAAGATCT CAGAGCCCCT GGACTTGGCC ACCATAGAGA GGCAGATCCT CACCGGCCAT 
TACAAGACCG TGGAGGCCTT TGACGCTGAC ATGCTCAAGG TGTTTCGGAA TGCTGAGAAG 
TACTACGGGC GTAAATCCCC AATTGGGAGA GATGTTTGCC GTCTACGGAA GACCTACTAC 
AACGCCAGGC ATGAGGCATC AGCCCAGATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG 
GCCGACAGCA GCGAAACATC TGTCTCTGAG AAGGAGAACG TGCATGAGAA GGATGACGAT 
GTGATCCGCT GTGTCTGTGG CCTCCACAAG GATGAGGGCC TCATGATCCA GTGTGACAAG 
TGCATGGTAT GGCAGCACTG TGACTGTATG GGGGTGAACT CAGACGTGGA GCATTACCTG 
TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC 
TATGCCCAGC CCGGCTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GTTGCTTCGT 
CAAGGTGACT GTGTGTACCT GATGCGAGAT AGCCGGCGCA CCCCTGATGG CCACCCAGTC 
CGCCAGTCCT ACCGACTGTT ATCACACATT AACCGAGATA AACTTGACAT CTTCCGCATT 
GAGAAGCTCT GGAAGAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA CTACTTCCGC 
CCCCACGAGA CCCACCACTC TCCGTCACGC CGCTTCTATC ACAATGAGCT ATTTAGGGTA 
CCACTCTATG AGATCATTCC CTTGGAGGCT GTGGTGGGGA CCTGCTGTGT ACTAGACCTT 
TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTATA CATCTGTGAC 
TATCGGCTTG ACAAGTCCGC ACACCTGTTT TACAAGATTC ACCGGAATCG CTATCCTGTC 
TGCACCAAAC CCTACGCCTT TGACCACTTC CCCAAGAAGC TCACTCCTAA AAGAGATTTC 
TCACCTCATT ATGTCCCAGA CAACTACAAG AGGAACGGAG GGCGGTCATC CTGGAAGTCT 
GAGCGCCCAA AGCCACCTCT AAAAGACCTG GGCCAGGAGG ATGACGCTCT GCCCTTGATT 
GAAGAGGTTC TGGCCAGCCA GGAGCAAGCC ACCAATGAGC TGCCCAGCCT GGAGGAGCCA 
GAACGGGAAG TGGCCCCTGT GGAAGTCAGC GAGGGTGAAA AGAAAGCAGA GGAAGGTGGT 
CCAGAATCTC AGGCTGCCTG TACCCCTGAG GAGCGACGGC ACAGCCAACG GGAACGGCTC 
AACCAGATCC TACTTGGCCT CCTGGAAAAA ATCCCTGGGA AAAATGCCAT CGATGTGACC 
TACTTGCTGG AGGAAGGGTC AGGCAGGAAG CTTCGAAGGC GCACTTTGTT CATCCCAGAG 
AACAGCTTTC GGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASH1L ( XM_537251.6 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_537251.2 Canis lupus familiaris ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X2, mRNA.

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CloneID	OCe09533
Clone ID Related Accession (Same CDS sequence)	XM_014115336.2 , XM_537251.6 , XM_014115338.2 , XM_537251.5 , XM_014115337.1 , XM_014115338.1 , XM_014115336.1 , XM_014115337.2
Accession Version	XM_537251.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8898bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	histone-lysine N-methyltransferase ASH1L
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_537251.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCTA GAAATACTGC TATGTTAGGA TTGGGTTCTG ACTCGGAAGG ATTTTCAAGG 
AAGAGTCCCT CTGCCCTTGG TACCGGCACA GTGGTCAGCA AGAGAGAAGG AGAGCTAGAG 
AGCAACACGA AAGAGGAAGA GGACCTTCGC AAGAGGAATT GGGACAGAAA CAGCGAAGCA 
GGGAAGGAGG ATGGTTTGAC TGATGCACAG CAACAGTTTT CAGTGAAAGA AACAAACTTT 
TCAGAGGGAA ACTTAAAATT GAAAATTGGC CTTCAGGCTA AGAGAACTAA AAAACCTCCA 
AAAAACTTGG AGAACTATGT ATGTCGACCT GCCATAAAAA CAACTATCAA GCACCCAAGG 
AAAGCACTTA AAGGTGGGAA GATGACAGAG GAGAAGGATG AGCACTGTCC TTCAAGACGG 
GACCCTTCAA AGTTGTACAA GAAAACAGGT GATGTTGCAG CCGTTGAATG CCAATCTGAA 
GAGAGCATCC ATCTTCATTC ACAGGGAGAA AACAGTCCTT TGTCTAAGAA GCTTTCTCCA 
GGACACTCAG AGATGACAGA TTACATGAAT GCAGCATCAT CTACTCTTGT TGGTAGCCGG 
GATCCTGATT TAAAGGATAG AGCATTACTT AATGGAGGAA CAAGTGTAAC AGAAAAGTTG 
GCACAGCTTA TTGCAACCTG TCCTCCCTCT AAGTCTTCCA AGACTAAACC GAAGAAGTTA 
GGAAGTGGCA CCAGCGCAGG ATTGGTTAGT AAGGATTTGA TCAGGAAAGC AGGTGTTGGC 
TCTGTAGCTG GAATAATACA TAAAGATTTA ATTAAAAAAC CAGCTATCAG CACAGCAGTT 
GGATTGGTAA CTAAAGATCC TGGGAAAAAG CCAGTGTTTA ATGCAACAGT AGGATTGGTC 
AATAAGGACT CTGTGAAAAA ACTAGGAGCT GGCACTACAG CGGTATTCAT TAATAAAGAC 
TTAGGCAGAA AGCCAGGGAC TGTCACTACA GTAGGACTGC TGAGCAAGGA TTCAGGAAAG 
AAGCTAGGAA TTGGTATTGT TCCAGGTTTA GTGAATAAGG AATCTGGAAA GAAGTTAGGA 
CTCGGCACTG TGGTCGGACT GGTTAATAAA GACTTGGGCA AGAAGTTGAG TTCTACTGTC 
GGTCTAGTGG CCAAGGACTG TGCAAAGAAG ATTGTAGCCA ATTCAACAAT GGGATTAGTT 
ACTAAAGACA TGGGAAAGAA ACTAGTGAGT TGTTCTATGG CAGGATTGGT CAATAAAGAT 
GCCATAAACC TTAAAGCCGA AGCACTGCTC CCCACTCAGG AACCACTTAA GGCTTCTTGT 
AGTACAAACA TCAGTAGTCA TGAAAATCAG GAACTTTCTG AATCCCTGAA AGATAGTACC 
ACCAGCAAAA CTTTTGAGAA GAATGTTATA CGGCAGAGTA AAGAAAGCAT ATTGGAAAAG 
TTCTCAGTTC GAAAAGAAAT CATTAATTTG GAGAAAGAAA TGTTTAACGA GGGAACATGC 
ATTCAGCAAG ACAGTTTCTC ATCCAATGAC AGGGGGCCTT ATGAAACCTC AAAGCATGAA 
AAGCAACCTC CCGTGTATTG CACTTCTCCA GACTTTCAAA TGGGAGCTGC TTCTGATGCA 
TCTATGGCAA AATCCCCATT TAGTGCAGTA GGAGAAAGCA ATCTCCCTTC CCCATCACCT 
ACTGTATCTG TTAATCCTTT AACCAGAAGT CCCCCTGAAA CTTCTTCACA GTTGGCTCCT 
AATCCGTTAC TTTTAAGTCC TACCACAGAA CTAATGGAAG ACATTTCTGA ATCCGTTGGG 
AAGAACCAGT TTACTTCTGA AAGTACCCAC TTGAACATTG GTCATAGGTC TGTGGGTCAC 
AGCATGAATA TTGAATGTAA AGGGATTGAT AAAGAGCTAA CTGATTCAAA AACTGCACAT 
ATAGATATTC CAAGAATAAG CTCTTCCCTG GGAAAAAAGC CAAGTTTGAC TTCTGAATCC 
AGTATTCACA CAATTACCCC TTCAGTTGTT AACTTCACTA GTTTATTTAG TAACAAGCCG 
TTTCTAAAAC TTGGTGCAGT ATCTGCTTCT GACAAACACT GCCAGGTTGC TGAAAGCCTA 
AGCTCTAGTT TGCAGTCCAA ACCATTAAAA AAAAGGAAAG GAAGAAAAGC TCGGTGGACT 
AAAGTGGTGG CAAGAAGCAC ATGCCGGTCT CCAAAAGGGC TAGATTTAGA AAGATCAGAG 
CTTTTTAAAA GTGTTTCATG TAGCTCACTA TCAAATAGTA ATTCTGAGCC AGCCAAGTTC 
ATGAAAAACA TTGGACCATC TTCATTTGTA GATCATGACT TCCTTAAACG CCGATTGCCA 
AAGTTGAGCA AGTCCACAAC TCCATCTCTT GCTCTCTTAA CTGATAGTGA AAAACCATCT 
CACAAGGCTT TTGCTACTCA CAAATTATCC TCCAGTATGT GTGTCTCTAG TGACCTTTTG 
TCTGATATTT ATAAGCCCAA AAGAGGAAGA CCTAAAGCTA AAGAGATGCC TCAACTGGAA 
GGTCCACCTA AAAGGACTTT AAAAATCCCT GCCTCTAAAG TTTTTTCTTT GCAGTCTAAG 
GAAGAACAAG AACCCCCAAT TTTACAGCCA GAAATTGAAA TTCCTTCCTT CAAACAAAGT 
CTTTCTGTAT CTCCTTTTCC AAAAAAGCGA GGCAGACCTA AGAGGCAGTT GAGGTCACCG 
GTCAAGATGA AGCCACCTGT ACTATCAGTG GCTCCATTTG TTGCCACGGA GAGTCCAAGC 
AAGCTGGAGT CTGAAAGTGA TAACCATCGA AGCAGCAGTG ATTTCTTTGA GAGTGAGGAT 
CAGCTTCAGG ACCCAGATGA TCTGGACGAC AGTCATAGGC CGGCTGTCTG CAGCATGAGT 
GACCTTGAGA TGGAACCAGA TAAAAAAATT ACAAAGAGAA ACAATGGACA ATTAATGAAA 
ACAATTATCC GCAAAATAAA TAAAATGAAG ACTTTAAAAA GGAAGAAACT GTTGAATCAG 
ATTCTTTCAA GCTCTGTAGA ATCAAGTAAT AAAGGGAAAG TGCAATCCAA ACTCCATAAT 
ACAGTGTCAA GTCTTGCTGC CACGTTTGGC TCTAAATTGG GCCAACAGAT AAATGTCAGC 
AAGAAAGGAA CCATTTACAT AGGAAAGAGA AGGGGTCGAA AACCAAAAAC TGTCTTAAAT 
GGCATTCTTT CTGGTAGCCC TACCAGCCTT GCTGTCCTTG AGCAAACTGC TCAACAGGCT 
GCCGGCTCAG CATTGGGACA GATACTTCCC CCTTTACTGC CTTCATCTGC TAGTAGCTCA 
GAGATTCTCC CATCGCCTGT TTGCTCTCAG TCTTCTGGGA CTAGTGGTGG TCAGAGCCCT 
GTAAGTAGTG ATGCAGGCTT TGTTGAACCC AGCTCAGTGC CATATTTGCA TTTACACTCC 
AGACAGGGCA GTATGATTCA GACTCTTGCG ATGAAAAAGG CCTCAAAGGG GAGGAGGCGA 
CTTTCTCCTC CTACTTTGTT GCCAAATTCT CCTTCACACT TGAGTGAACT CACCTCTCTG 
AAAGAAGCTA CTCCTTCTCC CATTAGTGAG TCTCATAGTG ATGAGACCAT TCCCAGTGAT 
AGTGGAATTG GAACAGATAA TAACAGCACA TCGGACAGAG CAGAGAAATT TTGTGGACAG 
AAAAAGAGGA GGCACTCTTT TGAACATGTT TCTCTGATTC CCCCTGAAAC CTCTACAGTC 
CTGAGCAGTC TTAAAGAAAA ACATAAACAT AAATGTAAGC GCCGGAATCA CGATTACCTC 
AGCTATGACA AGATGAAAAG GCAGAAACGA AAACGGAAAA AGAAATACCC CCAGCTCCGT 
AGCAGACAGG ATCCAGACTT TATTGCGGAT CTGGAGGAAT TGATAAGTCG GCTGAGTGAA 
ATTCGAATCA CCCATCGAAG CCATCATTTT ATCCCCCGAG ACCTGCTGCC AACTATTTTT 
CGAATCAACT TTAATAGTTT CTATACACAT CCTTCTTTCC CCTTAGACCC CTTGCACTAC 
ATTCGAAAAC CTGACTTAAA AAAGAAGAGA GGGAGACCTC CTAAGATGAG GGAGGCAATG 
GCTGAGATGC CCTTCATGCA CAGCCTTAGT TTTCCTCTTT CTAGTACTGG ATTCTATCCC 
TCCTATGGCA TGCCTTACTC TCCCTCACCC CTTACAGCCA CTCCCCTAGG ATTAGGTTAC 
TATGGAAGGT ACCCTCCCAC TCTCTATCCT CCTCCTCCTT CACCTTCTTT CACCCCCCCA 
CTTCCTCCTC CTTCCTATAT GCATGCTGGC CATTTACTTC TCAATCCAAC CAAATACCAT 
AAGAAAAAGC ATAAGCTACT TCGCCAGGAG GCCTTTCTAA CAACCAGCAG GACTCCCCTC 
CTTTCCATGA GTACCTACCC TAGTGTTCCT CCTGAGATGG CCTATGGTTG GATGGTTGAG 
CACAAACATA GGCATCGTCA CAAGCACAGA GAACACCGTT CTTCTGAGCA ACCCCAGGTT 
TCCATGGATG CTGGCTCTTC CAGATCTGTT TTGGAGTCTT TGAAGCGCTA TCGATTTGGA 
AAGGAAACTG TTGGAGAGCG ATATAAACAT AAGGAAAAGC ACCGATGTCA TATGTCCTGC 
CCTCATCTCT CTCCTTCGAA AAGCTTAATA AATAGAGAAG AACAATGGGT CCACCGAGAG 
CCTTCAGAAT CTAGTCCATT GGCTTTGGGA TTGCAAACAC CTTTACAGAT TGACTGTTCC 
GAAAGTTCTC CAAGTTTATC TCTTGGAGGA TTCACTCCCA ACTCTGATCC GGCCAGCAGT 
GATGAACATA CAAACCTTTT CACAAGTGCA ATAGTGCAAG GCAGCTGCAG AGTTTCAAAC 
CCTAACTCCA GTGGCCGGAA GAAATTAACT GATAGCCCTG GACTGTTTTC TGCACAGGAC 
ACTTCACTAA TTCGGCCTCA CAGAAAGGAG CCACTGCCTT CCAGCGAAAG GGCAGTACAG 
ACCATGACAG GTTCCCAGCC AACCTCAGAT AAGTCTTCCC AGCGACCATC AGAGAGCACA 
AACTGTAGCC CTACCCGGAA GAGGTCTTCA TCTGAGAGTA CTTCTTCAAC AGTAAACGGA 
CTTCCTTCTC GAAGTCCAAG ATTGCTTGCC CCTGGGGATG ACTCTGTGGA TAGTCTGCTG 
CAGCGGATGG TACAACATGA GGACCAAGAG CCCCTGGAGA AGAATCTAGA TGCCGTGATT 
GCATCTGCCT CGGGGCCACC TTCTTCCAGT CCAGGCCACA GCCACAGCAA GGAACGAGCC 
CTGGGAAAAC CAGATAGCCT TCTAGTGCCC GCAGCCCCCA GTGACTCTTG TAGCAGCAGC 
ATCTCACTCC TGTCTGAGAA GTTGCCGAGC AGCTGCTCAC CCCACCATAT CAAGAGGAGT 
GTCGTGGAAG CCATGCAGCG CCAAGCTCGG AAAATGTGCA ATTATGACAA AATCTTGGCC 
ACAAAGAAAA ACCTGGACCA TGTCAATAAA ATATTAAAAG CCAAAAAGCT TCAAAGACAG 
GCCAGGACAG GGAATAACTT CGTGAAACGC AGACCAGGGC GACCTCGGAA ATGCCCCCTC 
CAGGCAGTGG TGTCAATGCA AGCATTCCAG GCTGCCCGAT TCGTCAGCCC CGATGCTAAT 
GAAGGTGAAG AAAGAGCAGC CCTGCCCCTC CGTCCAGACA CAGTTACGGA TGTCATCGAG 
GCTGTTGTGC AGAGCGTAAA CCTGAGTCCA GAACATAAGA AGGGGCTGAA GAGGAAAAGT 
TGGCTGTTGG AAGAACAGAC CAAGAAAAAG CAGAAGCCAT TCCCAGAGGA AGAACAAGAG 
CATCCCAAGA GCTTTACTGA AGCAGCAGTT GAGATTCCCA GTCCTCCTGA AACCCCAGCC 
AAGCCTTCCG AACCTGAAAG TACCTTGCAG CCTGTGCTTT CTCTCATCCC AAGGGAAAAG 
AAGGCCCCAC GTCCCCCAAA GAAGAAGTAT CAGAAAGCAG GGCTGTATTC TGACGTTTAC 
AAAACTACAG ACCCAAAGAG CCGATTGATC CAATTAAAGA AAGAAAAGTT GGAATACACT 
CCAGGAGAGC ATGAGTATGG ATTATTCCCA GCCCCTATTC ATGTTGGAAA GTATCTAAGG 
CAAAAGAGAA TTGACTTCCA GCTTCCCTAT GACATCCTTT GGCAGTGGAA ACATAATCAG 
CTATACAAAA AGCCAGATGT CCCACTATAT AAAAAAATCC GTTCAAACGT CTACGTGGAT 
GTCAAACCCC TTTCTGGTTA CGAGGCCACC ACCTGTAACT GTAAGAAGCC AGAGGATGAC 
ACCGGGAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCT 
AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGAGGCA TGAGTGGGTG 
CAGTGTCTAG AACGATTTCG AGCTGAGGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC 
CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGGGAGG TTGTCAGCGA ACAGGAGTTC 
AGGAACAGAA TGATTGAGCA GTATCATAAT CACAGTGACC ACTATTGCCT GAACCTGGAT 
AGTGGGATGG TGATTGACAG TTATCGGATG GGTAATGAGG CCCGATTCAT CAACCACAGC 
TGTGACCCAA ACTGTGAAAT GCAGAAATGG TCTGTCAATG GAGTATACCG GATTGGACTG 
TATGCTCTTA AAGATATGCC AGCTGGTACC GAGCTCACTT ATGATTACAA TTTCCATTCC 
TTTAATGTTG AGAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT 
GGGGGCAAGA GTCAGCGCGT GAACGGACTC GCCAGCAGCA AAAGCAGCCA GCCTGTGGCT 
ACCCATAAAA AGTCTGGACG GTCAAAAGAG AAGAGAAAGT CAAAGCACAA GCTGAAGAAA 
AGGAGAGGCC ACCTCTCCGA AGAACCCAGT GAAAACATCA ACACTCCAAC AAGATTGACC 
CCCCAATTAC AAATGAAGCC AATGTCTAAT CGAGAGAGGA ACTTCGTGTT AAAGCATCAT 
GTATTCTTGG TCCGAAACTG GGAGAAGATC CGACAGAAAC AAGAGGAAGT AAAGCATACC 
AGCGATAATC TCCACTCTGC ATCATTATAC ACCCGCTGGA ATGGCATCTG CCGAGATGAT 
GGGAACATCA AGTCTGACGT CTTCATGACC CAGTTCTCCG CCCTGCAGAC AGCACGATCA 
GTTCGAACAA GACGCCTGGC AGCAGCAGAG GAAAACATCG AGGTGGCACG GGCAGCCCGC 
CTCGCCCAAA TCTTCAAAGA GATCTGTGAT GGCATCATCT CCTACAAAGA TTCTTCCCGA 
CAAGCTTTGG CAGCACCCCT CTTGAACCTC CCCCCAAAGA AAAAGAATGC TGATTACTAT 
GAGAAGATCT CAGAGCCCCT GGACTTGGCC ACCATAGAGA GGCAGATCCT CACCGGCCAT 
TACAAGACCG TGGAGGCCTT TGACGCTGAC ATGCTCAAGG TGTTTCGGAA TGCTGAGAAG 
TACTACGGGC GTAAATCCCC AATTGGGAGA GATGTTTGCC GTCTACGGAA GACCTACTAC 
AACGCCAGGC ATGAGGCATC AGCCCAGATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG 
GCCGACAGCA GCGAAACATC TGTCTCTGAG AAGGAGAACG TGCATGAGAA GGATGACGAT 
GTGATCCGCT GTGTCTGTGG CCTCCACAAG GATGAGGGCC TCATGATCCA GTGTGACAAG 
TGCATGGTAT GGCAGCACTG TGACTGTATG GGGGTGAACT CAGACGTGGA GCATTACCTG 
TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC 
TATGCCCAGC CCGGCTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GTTGCTTCGT 
CAAGGTGACT GTGTGTACCT GATGCGAGAT AGCCGGCGCA CCCCTGATGG CCACCCAGTC 
CGCCAGTCCT ACCGACTGTT ATCACACATT AACCGAGATA AACTTGACAT CTTCCGCATT 
GAGAAGCTCT GGAAGAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA CTACTTCCGC 
CCCCACGAGA CCCACCACTC TCCGTCACGC CGCTTCTATC ACAATGAGCT ATTTAGGGTA 
CCACTCTATG AGATCATTCC CTTGGAGGCT GTGGTGGGGA CCTGCTGTGT ACTAGACCTT 
TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTATA CATCTGTGAC 
TATCGGCTTG ACAAGTCCGC ACACCTGTTT TACAAGATTC ACCGGAATCG CTATCCTGTC 
TGCACCAAAC CCTACGCCTT TGACCACTTC CCCAAGAAGC TCACTCCTAA AAGAGATTTC 
TCACCTCATT ATGTCCCAGA CAACTACAAG AGGAACGGAG GGCGGTCATC CTGGAAGTCT 
GAGCGCCCAA AGCCACCTCT AAAAGACCTG GGCCAGGAGG ATGACGCTCT GCCCTTGATT 
GAAGAGGTTC TGGCCAGCCA GGAGCAAGCC ACCAATGAGC TGCCCAGCCT GGAGGAGCCA 
GAACGGGAAG TGGCCCCTGT GGAAGTCAGC GAGGGTGAAA AGAAAGCAGA GGAAGGTGGT 
CCAGAATCTC AGGCTGCCTG TACCCCTGAG GAGCGACGGC ACAGCCAACG GGAACGGCTC 
AACCAGATCC TACTTGGCCT CCTGGAAAAA ATCCCTGGGA AAAATGCCAT CGATGTGACC 
TACTTGCTGG AGGAAGGGTC AGGCAGGAAG CTTCGAAGGC GCACTTTGTT CATCCCAGAG 
AACAGCTTTC GGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_537251.2
CDS	516..9413
Translation	MDPRNTAMLGLGSDSEGFSRKSPSALGTGTVVSKREGELESNTKEEEDLRKRNWDRNSEAGKEDGLTDAQQQFSVKETNFSEGNLKLKIGLQAKRTKKPPKNLENYVCRPAIKTTIKHPRKALKGGKMTEEKDEHCPSRRDPSKLYKKTGDVAAVECQSEESIHLHSQGENSPLSKKLSPGHSEMTDYMNAASSTLVGSRDPDLKDRALLNGGTSVTEKLAQLIATCPPSKSSKTKPKKLGSGTSAGLVSKDLIRKAGVGSVAGIIHKDLIKKPAISTAVGLVTKDPGKKPVFNATVGLVNKDSVKKLGAGTTAVFINKDLGRKPGTVTTVGLLSKDSGKKLGIGIVPGLVNKESGKKLGLGTVVGLVNKDLGKKLSSTVGLVAKDCAKKIVANSTMGLVTKDMGKKLVSCSMAGLVNKDAINLKAEALLPTQEPLKASCSTNISSHENQELSESLKDSTTSKTFEKNVIRQSKESILEKFSVRKEIINLEKEMFNEGTCIQQDSFSSNDRGPYETSKHEKQPPVYCTSPDFQMGAASDASMAKSPFSAVGESNLPSPSPTVSVNPLTRSPPETSSQLAPNPLLLSPTTELMEDISESVGKNQFTSESTHLNIGHRSVGHSMNIECKGIDKELTDSKTAHIDIPRISSSLGKKPSLTSESSIHTITPSVVNFTSLFSNKPFLKLGAVSASDKHCQVAESLSSSLQSKPLKKRKGRKARWTKVVARSTCRSPKGLDLERSELFKSVSCSSLSNSNSEPAKFMKNIGPSSFVDHDFLKRRLPKLSKSTTPSLALLTDSEKPSHKAFATHKLSSSMCVSSDLLSDIYKPKRGRPKAKEMPQLEGPPKRTLKIPASKVFSLQSKEEQEPPILQPEIEIPSFKQSLSVSPFPKKRGRPKRQLRSPVKMKPPVLSVAPFVATESPSKLESESDNHRSSSDFFESEDQLQDPDDLDDSHRPAVCSMSDLEMEPDKKITKRNNGQLMKTIIRKINKMKTLKRKKLLNQILSSSVESSNKGKVQSKLHNTVSSLAATFGSKLGQQINVSKKGTIYIGKRRGRKPKTVLNGILSGSPTSLAVLEQTAQQAAGSALGQILPPLLPSSASSSEILPSPVCSQSSGTSGGQSPVSSDAGFVEPSSVPYLHLHSRQGSMIQTLAMKKASKGRRRLSPPTLLPNSPSHLSELTSLKEATPSPISESHSDETIPSDSGIGTDNNSTSDRAEKFCGQKKRRHSFEHVSLIPPETSTVLSSLKEKHKHKCKRRNHDYLSYDKMKRQKRKRKKKYPQLRSRQDPDFIADLEELISRLSEIRITHRSHHFIPRDLLPTIFRINFNSFYTHPSFPLDPLHYIRKPDLKKKRGRPPKMREAMAEMPFMHSLSFPLSSTGFYPSYGMPYSPSPLTATPLGLGYYGRYPPTLYPPPPSPSFTPPLPPPSYMHAGHLLLNPTKYHKKKHKLLRQEAFLTTSRTPLLSMSTYPSVPPEMAYGWMVEHKHRHRHKHREHRSSEQPQVSMDAGSSRSVLESLKRYRFGKETVGERYKHKEKHRCHMSCPHLSPSKSLINREEQWVHREPSESSPLALGLQTPLQIDCSESSPSLSLGGFTPNSDPASSDEHTNLFTSAIVQGSCRVSNPNSSGRKKLTDSPGLFSAQDTSLIRPHRKEPLPSSERAVQTMTGSQPTSDKSSQRPSESTNCSPTRKRSSSESTSSTVNGLPSRSPRLLAPGDDSVDSLLQRMVQHEDQEPLEKNLDAVIASASGPPSSSPGHSHSKERALGKPDSLLVPAAPSDSCSSSISLLSEKLPSSCSPHHIKRSVVEAMQRQARKMCNYDKILATKKNLDHVNKILKAKKLQRQARTGNNFVKRRPGRPRKCPLQAVVSMQAFQAARFVSPDANEGEERAALPLRPDTVTDVIEAVVQSVNLSPEHKKGLKRKSWLLEEQTKKKQKPFPEEEQEHPKSFTEAAVEIPSPPETPAKPSEPESTLQPVLSLIPREKKAPRPPKKKYQKAGLYSDVYKTTDPKSRLIQLKKEKLEYTPGEHEYGLFPAPIHVGKYLRQKRIDFQLPYDILWQWKHNQLYKKPDVPLYKKIRSNVYVDVKPLSGYEATTCNCKKPEDDTGKGCVDDCLNRMIFAECSPNTCPCGEQCCNQRIQRHEWVQCLERFRAEEKGWGIRTKEPLKAGQFIIEYLGEVVSEQEFRNRMIEQYHNHSDHYCLNLDSGMVIDSYRMGNEARFINHSCDPNCEMQKWSVNGVYRIGLYALKDMPAGTELTYDYNFHSFNVEKQQLCKCGFEKCRGIIGGKSQRVNGLASSKSSQPVATHKKSGRSKEKRKSKHKLKKRRGHLSEEPSENINTPTRLTPQLQMKPMSNRERNFVLKHHVFLVRNWEKIRQKQEEVKHTSDNLHSASLYTRWNGICRDDGNIKSDVFMTQFSALQTARSVRTRRLAAAEENIEVARAARLAQIFKEICDGIISYKDSSRQALAAPLLNLPPKKKNADYYEKISEPLDLATIERQILTGHYKTVEAFDADMLKVFRNAEKYYGRKSPIGRDVCRLRKTYYNARHEASAQIDEIVGETASEADSSETSVSEKENVHEKDDDVIRCVCGLHKDEGLMIQCDKCMVWQHCDCMGVNSDVEHYLCEQCDPRPVDREVPMIPRPHYAQPGCVYFICLLRDDLLLRQGDCVYLMRDSRRTPDGHPVRQSYRLLSHINRDKLDIFRIEKLWKNEKEERFAFGHHYFRPHETHHSPSRRFYHNELFRVPLYEIIPLEAVVGTCCVLDLYTYCKGRPKGVKEQDVYICDYRLDKSAHLFYKIHRNRYPVCTKPYAFDHFPKKLTPKRDFSPHYVPDNYKRNGGRSSWKSERPKPPLKDLGQEDDALPLIEEVLASQEQATNELPSLEEPEREVAPVEVSEGEKKAEEGGPESQAACTPEERRHSQRERLNQILLGLLEKIPGKNAIDVTYLLEEGSGRKLRRRTLFIPENSFRK

Target ORF information:

RefSeq Version	XM_537251.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_537251.6

ORF Insert Sequence:

ATGGACCCTA GAAATACTGC TATGTTAGGA TTGGGTTCTG ACTCGGAAGG ATTTTCAAGG 
AAGAGTCCCT CTGCCCTTGG TACCGGCACA GTGGTCAGCA AGAGAGAAGG AGAGCTAGAG 
AGCAACACGA AAGAGGAAGA GGACCTTCGC AAGAGGAATT GGGACAGAAA CAGCGAAGCA 
GGGAAGGAGG ATGGTTTGAC TGATGCACAG CAACAGTTTT CAGTGAAAGA AACAAACTTT 
TCAGAGGGAA ACTTAAAATT GAAAATTGGC CTTCAGGCTA AGAGAACTAA AAAACCTCCA 
AAAAACTTGG AGAACTATGT ATGTCGACCT GCCATAAAAA CAACTATCAA GCACCCAAGG 
AAAGCACTTA AAGGTGGGAA GATGACAGAG GAGAAGGATG AGCACTGTCC TTCAAGACGG 
GACCCTTCAA AGTTGTACAA GAAAACAGGT GATGTTGCAG CCGTTGAATG CCAATCTGAA 
GAGAGCATCC ATCTTCATTC ACAGGGAGAA AACAGTCCTT TGTCTAAGAA GCTTTCTCCA 
GGACACTCAG AGATGACAGA TTACATGAAT GCAGCATCAT CTACTCTTGT TGGTAGCCGG 
GATCCTGATT TAAAGGATAG AGCATTACTT AATGGAGGAA CAAGTGTAAC AGAAAAGTTG 
GCACAGCTTA TTGCAACCTG TCCTCCCTCT AAGTCTTCCA AGACTAAACC GAAGAAGTTA 
GGAAGTGGCA CCAGCGCAGG ATTGGTTAGT AAGGATTTGA TCAGGAAAGC AGGTGTTGGC 
TCTGTAGCTG GAATAATACA TAAAGATTTA ATTAAAAAAC CAGCTATCAG CACAGCAGTT 
GGATTGGTAA CTAAAGATCC TGGGAAAAAG CCAGTGTTTA ATGCAACAGT AGGATTGGTC 
AATAAGGACT CTGTGAAAAA ACTAGGAGCT GGCACTACAG CGGTATTCAT TAATAAAGAC 
TTAGGCAGAA AGCCAGGGAC TGTCACTACA GTAGGACTGC TGAGCAAGGA TTCAGGAAAG 
AAGCTAGGAA TTGGTATTGT TCCAGGTTTA GTGAATAAGG AATCTGGAAA GAAGTTAGGA 
CTCGGCACTG TGGTCGGACT GGTTAATAAA GACTTGGGCA AGAAGTTGAG TTCTACTGTC 
GGTCTAGTGG CCAAGGACTG TGCAAAGAAG ATTGTAGCCA ATTCAACAAT GGGATTAGTT 
ACTAAAGACA TGGGAAAGAA ACTAGTGAGT TGTTCTATGG CAGGATTGGT CAATAAAGAT 
GCCATAAACC TTAAAGCCGA AGCACTGCTC CCCACTCAGG AACCACTTAA GGCTTCTTGT 
AGTACAAACA TCAGTAGTCA TGAAAATCAG GAACTTTCTG AATCCCTGAA AGATAGTACC 
ACCAGCAAAA CTTTTGAGAA GAATGTTATA CGGCAGAGTA AAGAAAGCAT ATTGGAAAAG 
TTCTCAGTTC GAAAAGAAAT CATTAATTTG GAGAAAGAAA TGTTTAACGA GGGAACATGC 
ATTCAGCAAG ACAGTTTCTC ATCCAATGAC AGGGGGCCTT ATGAAACCTC AAAGCATGAA 
AAGCAACCTC CCGTGTATTG CACTTCTCCA GACTTTCAAA TGGGAGCTGC TTCTGATGCA 
TCTATGGCAA AATCCCCATT TAGTGCAGTA GGAGAAAGCA ATCTCCCTTC CCCATCACCT 
ACTGTATCTG TTAATCCTTT AACCAGAAGT CCCCCTGAAA CTTCTTCACA GTTGGCTCCT 
AATCCGTTAC TTTTAAGTCC TACCACAGAA CTAATGGAAG ACATTTCTGA ATCCGTTGGG 
AAGAACCAGT TTACTTCTGA AAGTACCCAC TTGAACATTG GTCATAGGTC TGTGGGTCAC 
AGCATGAATA TTGAATGTAA AGGGATTGAT AAAGAGCTAA CTGATTCAAA AACTGCACAT 
ATAGATATTC CAAGAATAAG CTCTTCCCTG GGAAAAAAGC CAAGTTTGAC TTCTGAATCC 
AGTATTCACA CAATTACCCC TTCAGTTGTT AACTTCACTA GTTTATTTAG TAACAAGCCG 
TTTCTAAAAC TTGGTGCAGT ATCTGCTTCT GACAAACACT GCCAGGTTGC TGAAAGCCTA 
AGCTCTAGTT TGCAGTCCAA ACCATTAAAA AAAAGGAAAG GAAGAAAAGC TCGGTGGACT 
AAAGTGGTGG CAAGAAGCAC ATGCCGGTCT CCAAAAGGGC TAGATTTAGA AAGATCAGAG 
CTTTTTAAAA GTGTTTCATG TAGCTCACTA TCAAATAGTA ATTCTGAGCC AGCCAAGTTC 
ATGAAAAACA TTGGACCATC TTCATTTGTA GATCATGACT TCCTTAAACG CCGATTGCCA 
AAGTTGAGCA AGTCCACAAC TCCATCTCTT GCTCTCTTAA CTGATAGTGA AAAACCATCT 
CACAAGGCTT TTGCTACTCA CAAATTATCC TCCAGTATGT GTGTCTCTAG TGACCTTTTG 
TCTGATATTT ATAAGCCCAA AAGAGGAAGA CCTAAAGCTA AAGAGATGCC TCAACTGGAA 
GGTCCACCTA AAAGGACTTT AAAAATCCCT GCCTCTAAAG TTTTTTCTTT GCAGTCTAAG 
GAAGAACAAG AACCCCCAAT TTTACAGCCA GAAATTGAAA TTCCTTCCTT CAAACAAAGT 
CTTTCTGTAT CTCCTTTTCC AAAAAAGCGA GGCAGACCTA AGAGGCAGTT GAGGTCACCG 
GTCAAGATGA AGCCACCTGT ACTATCAGTG GCTCCATTTG TTGCCACGGA GAGTCCAAGC 
AAGCTGGAGT CTGAAAGTGA TAACCATCGA AGCAGCAGTG ATTTCTTTGA GAGTGAGGAT 
CAGCTTCAGG ACCCAGATGA TCTGGACGAC AGTCATAGGC CGGCTGTCTG CAGCATGAGT 
GACCTTGAGA TGGAACCAGA TAAAAAAATT ACAAAGAGAA ACAATGGACA ATTAATGAAA 
ACAATTATCC GCAAAATAAA TAAAATGAAG ACTTTAAAAA GGAAGAAACT GTTGAATCAG 
ATTCTTTCAA GCTCTGTAGA ATCAAGTAAT AAAGGGAAAG TGCAATCCAA ACTCCATAAT 
ACAGTGTCAA GTCTTGCTGC CACGTTTGGC TCTAAATTGG GCCAACAGAT AAATGTCAGC 
AAGAAAGGAA CCATTTACAT AGGAAAGAGA AGGGGTCGAA AACCAAAAAC TGTCTTAAAT 
GGCATTCTTT CTGGTAGCCC TACCAGCCTT GCTGTCCTTG AGCAAACTGC TCAACAGGCT 
GCCGGCTCAG CATTGGGACA GATACTTCCC CCTTTACTGC CTTCATCTGC TAGTAGCTCA 
GAGATTCTCC CATCGCCTGT TTGCTCTCAG TCTTCTGGGA CTAGTGGTGG TCAGAGCCCT 
GTAAGTAGTG ATGCAGGCTT TGTTGAACCC AGCTCAGTGC CATATTTGCA TTTACACTCC 
AGACAGGGCA GTATGATTCA GACTCTTGCG ATGAAAAAGG CCTCAAAGGG GAGGAGGCGA 
CTTTCTCCTC CTACTTTGTT GCCAAATTCT CCTTCACACT TGAGTGAACT CACCTCTCTG 
AAAGAAGCTA CTCCTTCTCC CATTAGTGAG TCTCATAGTG ATGAGACCAT TCCCAGTGAT 
AGTGGAATTG GAACAGATAA TAACAGCACA TCGGACAGAG CAGAGAAATT TTGTGGACAG 
AAAAAGAGGA GGCACTCTTT TGAACATGTT TCTCTGATTC CCCCTGAAAC CTCTACAGTC 
CTGAGCAGTC TTAAAGAAAA ACATAAACAT AAATGTAAGC GCCGGAATCA CGATTACCTC 
AGCTATGACA AGATGAAAAG GCAGAAACGA AAACGGAAAA AGAAATACCC CCAGCTCCGT 
AGCAGACAGG ATCCAGACTT TATTGCGGAT CTGGAGGAAT TGATAAGTCG GCTGAGTGAA 
ATTCGAATCA CCCATCGAAG CCATCATTTT ATCCCCCGAG ACCTGCTGCC AACTATTTTT 
CGAATCAACT TTAATAGTTT CTATACACAT CCTTCTTTCC CCTTAGACCC CTTGCACTAC 
ATTCGAAAAC CTGACTTAAA AAAGAAGAGA GGGAGACCTC CTAAGATGAG GGAGGCAATG 
GCTGAGATGC CCTTCATGCA CAGCCTTAGT TTTCCTCTTT CTAGTACTGG ATTCTATCCC 
TCCTATGGCA TGCCTTACTC TCCCTCACCC CTTACAGCCA CTCCCCTAGG ATTAGGTTAC 
TATGGAAGGT ACCCTCCCAC TCTCTATCCT CCTCCTCCTT CACCTTCTTT CACCCCCCCA 
CTTCCTCCTC CTTCCTATAT GCATGCTGGC CATTTACTTC TCAATCCAAC CAAATACCAT 
AAGAAAAAGC ATAAGCTACT TCGCCAGGAG GCCTTTCTAA CAACCAGCAG GACTCCCCTC 
CTTTCCATGA GTACCTACCC TAGTGTTCCT CCTGAGATGG CCTATGGTTG GATGGTTGAG 
CACAAACATA GGCATCGTCA CAAGCACAGA GAACACCGTT CTTCTGAGCA ACCCCAGGTT 
TCCATGGATG CTGGCTCTTC CAGATCTGTT TTGGAGTCTT TGAAGCGCTA TCGATTTGGA 
AAGGAAACTG TTGGAGAGCG ATATAAACAT AAGGAAAAGC ACCGATGTCA TATGTCCTGC 
CCTCATCTCT CTCCTTCGAA AAGCTTAATA AATAGAGAAG AACAATGGGT CCACCGAGAG 
CCTTCAGAAT CTAGTCCATT GGCTTTGGGA TTGCAAACAC CTTTACAGAT TGACTGTTCC 
GAAAGTTCTC CAAGTTTATC TCTTGGAGGA TTCACTCCCA ACTCTGATCC GGCCAGCAGT 
GATGAACATA CAAACCTTTT CACAAGTGCA ATAGTGCAAG GCAGCTGCAG AGTTTCAAAC 
CCTAACTCCA GTGGCCGGAA GAAATTAACT GATAGCCCTG GACTGTTTTC TGCACAGGAC 
ACTTCACTAA TTCGGCCTCA CAGAAAGGAG CCACTGCCTT CCAGCGAAAG GGCAGTACAG 
ACCATGACAG GTTCCCAGCC AACCTCAGAT AAGTCTTCCC AGCGACCATC AGAGAGCACA 
AACTGTAGCC CTACCCGGAA GAGGTCTTCA TCTGAGAGTA CTTCTTCAAC AGTAAACGGA 
CTTCCTTCTC GAAGTCCAAG ATTGCTTGCC CCTGGGGATG ACTCTGTGGA TAGTCTGCTG 
CAGCGGATGG TACAACATGA GGACCAAGAG CCCCTGGAGA AGAATCTAGA TGCCGTGATT 
GCATCTGCCT CGGGGCCACC TTCTTCCAGT CCAGGCCACA GCCACAGCAA GGAACGAGCC 
CTGGGAAAAC CAGATAGCCT TCTAGTGCCC GCAGCCCCCA GTGACTCTTG TAGCAGCAGC 
ATCTCACTCC TGTCTGAGAA GTTGCCGAGC AGCTGCTCAC CCCACCATAT CAAGAGGAGT 
GTCGTGGAAG CCATGCAGCG CCAAGCTCGG AAAATGTGCA ATTATGACAA AATCTTGGCC 
ACAAAGAAAA ACCTGGACCA TGTCAATAAA ATATTAAAAG CCAAAAAGCT TCAAAGACAG 
GCCAGGACAG GGAATAACTT CGTGAAACGC AGACCAGGGC GACCTCGGAA ATGCCCCCTC 
CAGGCAGTGG TGTCAATGCA AGCATTCCAG GCTGCCCGAT TCGTCAGCCC CGATGCTAAT 
GAAGGTGAAG AAAGAGCAGC CCTGCCCCTC CGTCCAGACA CAGTTACGGA TGTCATCGAG 
GCTGTTGTGC AGAGCGTAAA CCTGAGTCCA GAACATAAGA AGGGGCTGAA GAGGAAAAGT 
TGGCTGTTGG AAGAACAGAC CAAGAAAAAG CAGAAGCCAT TCCCAGAGGA AGAACAAGAG 
CATCCCAAGA GCTTTACTGA AGCAGCAGTT GAGATTCCCA GTCCTCCTGA AACCCCAGCC 
AAGCCTTCCG AACCTGAAAG TACCTTGCAG CCTGTGCTTT CTCTCATCCC AAGGGAAAAG 
AAGGCCCCAC GTCCCCCAAA GAAGAAGTAT CAGAAAGCAG GGCTGTATTC TGACGTTTAC 
AAAACTACAG ACCCAAAGAG CCGATTGATC CAATTAAAGA AAGAAAAGTT GGAATACACT 
CCAGGAGAGC ATGAGTATGG ATTATTCCCA GCCCCTATTC ATGTTGGAAA GTATCTAAGG 
CAAAAGAGAA TTGACTTCCA GCTTCCCTAT GACATCCTTT GGCAGTGGAA ACATAATCAG 
CTATACAAAA AGCCAGATGT CCCACTATAT AAAAAAATCC GTTCAAACGT CTACGTGGAT 
GTCAAACCCC TTTCTGGTTA CGAGGCCACC ACCTGTAACT GTAAGAAGCC AGAGGATGAC 
ACCGGGAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCT 
AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGAGGCA TGAGTGGGTG 
CAGTGTCTAG AACGATTTCG AGCTGAGGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC 
CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGGGAGG TTGTCAGCGA ACAGGAGTTC 
AGGAACAGAA TGATTGAGCA GTATCATAAT CACAGTGACC ACTATTGCCT GAACCTGGAT 
AGTGGGATGG TGATTGACAG TTATCGGATG GGTAATGAGG CCCGATTCAT CAACCACAGC 
TGTGACCCAA ACTGTGAAAT GCAGAAATGG TCTGTCAATG GAGTATACCG GATTGGACTG 
TATGCTCTTA AAGATATGCC AGCTGGTACC GAGCTCACTT ATGATTACAA TTTCCATTCC 
TTTAATGTTG AGAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT 
GGGGGCAAGA GTCAGCGCGT GAACGGACTC GCCAGCAGCA AAAGCAGCCA GCCTGTGGCT 
ACCCATAAAA AGTCTGGACG GTCAAAAGAG AAGAGAAAGT CAAAGCACAA GCTGAAGAAA 
AGGAGAGGCC ACCTCTCCGA AGAACCCAGT GAAAACATCA ACACTCCAAC AAGATTGACC 
CCCCAATTAC AAATGAAGCC AATGTCTAAT CGAGAGAGGA ACTTCGTGTT AAAGCATCAT 
GTATTCTTGG TCCGAAACTG GGAGAAGATC CGACAGAAAC AAGAGGAAGT AAAGCATACC 
AGCGATAATC TCCACTCTGC ATCATTATAC ACCCGCTGGA ATGGCATCTG CCGAGATGAT 
GGGAACATCA AGTCTGACGT CTTCATGACC CAGTTCTCCG CCCTGCAGAC AGCACGATCA 
GTTCGAACAA GACGCCTGGC AGCAGCAGAG GAAAACATCG AGGTGGCACG GGCAGCCCGC 
CTCGCCCAAA TCTTCAAAGA GATCTGTGAT GGCATCATCT CCTACAAAGA TTCTTCCCGA 
CAAGCTTTGG CAGCACCCCT CTTGAACCTC CCCCCAAAGA AAAAGAATGC TGATTACTAT 
GAGAAGATCT CAGAGCCCCT GGACTTGGCC ACCATAGAGA GGCAGATCCT CACCGGCCAT 
TACAAGACCG TGGAGGCCTT TGACGCTGAC ATGCTCAAGG TGTTTCGGAA TGCTGAGAAG 
TACTACGGGC GTAAATCCCC AATTGGGAGA GATGTTTGCC GTCTACGGAA GACCTACTAC 
AACGCCAGGC ATGAGGCATC AGCCCAGATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG 
GCCGACAGCA GCGAAACATC TGTCTCTGAG AAGGAGAACG TGCATGAGAA GGATGACGAT 
GTGATCCGCT GTGTCTGTGG CCTCCACAAG GATGAGGGCC TCATGATCCA GTGTGACAAG 
TGCATGGTAT GGCAGCACTG TGACTGTATG GGGGTGAACT CAGACGTGGA GCATTACCTG 
TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC 
TATGCCCAGC CCGGCTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GTTGCTTCGT 
CAAGGTGACT GTGTGTACCT GATGCGAGAT AGCCGGCGCA CCCCTGATGG CCACCCAGTC 
CGCCAGTCCT ACCGACTGTT ATCACACATT AACCGAGATA AACTTGACAT CTTCCGCATT 
GAGAAGCTCT GGAAGAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA CTACTTCCGC 
CCCCACGAGA CCCACCACTC TCCGTCACGC CGCTTCTATC ACAATGAGCT ATTTAGGGTA 
CCACTCTATG AGATCATTCC CTTGGAGGCT GTGGTGGGGA CCTGCTGTGT ACTAGACCTT 
TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTATA CATCTGTGAC 
TATCGGCTTG ACAAGTCCGC ACACCTGTTT TACAAGATTC ACCGGAATCG CTATCCTGTC 
TGCACCAAAC CCTACGCCTT TGACCACTTC CCCAAGAAGC TCACTCCTAA AAGAGATTTC 
TCACCTCATT ATGTCCCAGA CAACTACAAG AGGAACGGAG GGCGGTCATC CTGGAAGTCT 
GAGCGCCCAA AGCCACCTCT AAAAGACCTG GGCCAGGAGG ATGACGCTCT GCCCTTGATT 
GAAGAGGTTC TGGCCAGCCA GGAGCAAGCC ACCAATGAGC TGCCCAGCCT GGAGGAGCCA 
GAACGGGAAG TGGCCCCTGT GGAAGTCAGC GAGGGTGAAA AGAAAGCAGA GGAAGGTGGT 
CCAGAATCTC AGGCTGCCTG TACCCCTGAG GAGCGACGGC ACAGCCAACG GGAACGGCTC 
AACCAGATCC TACTTGGCCT CCTGGAAAAA ATCCCTGGGA AAAATGCCAT CGATGTGACC 
TACTTGCTGG AGGAAGGGTC AGGCAGGAAG CTTCGAAGGC GCACTTTGTT CATCCCAGAG 
AACAGCTTTC GGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASH1L ( XM_014115338.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013970813.1 Canis lupus familiaris ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X3, mRNA.

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CloneID	OCe09533
Clone ID Related Accession (Same CDS sequence)	XM_014115336.2 , XM_537251.6 , XM_014115338.2 , XM_537251.5 , XM_014115337.1 , XM_014115338.1 , XM_014115336.1 , XM_014115337.2
Accession Version	XM_014115338.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8898bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	histone-lysine N-methyltransferase ASH1L
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014115338.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCTA GAAATACTGC TATGTTAGGA TTGGGTTCTG ACTCGGAAGG ATTTTCAAGG 
AAGAGTCCCT CTGCCCTTGG TACCGGCACA GTGGTCAGCA AGAGAGAAGG AGAGCTAGAG 
AGCAACACGA AAGAGGAAGA GGACCTTCGC AAGAGGAATT GGGACAGAAA CAGCGAAGCA 
GGGAAGGAGG ATGGTTTGAC TGATGCACAG CAACAGTTTT CAGTGAAAGA AACAAACTTT 
TCAGAGGGAA ACTTAAAATT GAAAATTGGC CTTCAGGCTA AGAGAACTAA AAAACCTCCA 
AAAAACTTGG AGAACTATGT ATGTCGACCT GCCATAAAAA CAACTATCAA GCACCCAAGG 
AAAGCACTTA AAGGTGGGAA GATGACAGAG GAGAAGGATG AGCACTGTCC TTCAAGACGG 
GACCCTTCAA AGTTGTACAA GAAAACAGGT GATGTTGCAG CCGTTGAATG CCAATCTGAA 
GAGAGCATCC ATCTTCATTC ACAGGGAGAA AACAGTCCTT TGTCTAAGAA GCTTTCTCCA 
GGACACTCAG AGATGACAGA TTACATGAAT GCAGCATCAT CTACTCTTGT TGGTAGCCGG 
GATCCTGATT TAAAGGATAG AGCATTACTT AATGGAGGAA CAAGTGTAAC AGAAAAGTTG 
GCACAGCTTA TTGCAACCTG TCCTCCCTCT AAGTCTTCCA AGACTAAACC GAAGAAGTTA 
GGAAGTGGCA CCAGCGCAGG ATTGGTTAGT AAGGATTTGA TCAGGAAAGC AGGTGTTGGC 
TCTGTAGCTG GAATAATACA TAAAGATTTA ATTAAAAAAC CAGCTATCAG CACAGCAGTT 
GGATTGGTAA CTAAAGATCC TGGGAAAAAG CCAGTGTTTA ATGCAACAGT AGGATTGGTC 
AATAAGGACT CTGTGAAAAA ACTAGGAGCT GGCACTACAG CGGTATTCAT TAATAAAGAC 
TTAGGCAGAA AGCCAGGGAC TGTCACTACA GTAGGACTGC TGAGCAAGGA TTCAGGAAAG 
AAGCTAGGAA TTGGTATTGT TCCAGGTTTA GTGAATAAGG AATCTGGAAA GAAGTTAGGA 
CTCGGCACTG TGGTCGGACT GGTTAATAAA GACTTGGGCA AGAAGTTGAG TTCTACTGTC 
GGTCTAGTGG CCAAGGACTG TGCAAAGAAG ATTGTAGCCA ATTCAACAAT GGGATTAGTT 
ACTAAAGACA TGGGAAAGAA ACTAGTGAGT TGTTCTATGG CAGGATTGGT CAATAAAGAT 
GCCATAAACC TTAAAGCCGA AGCACTGCTC CCCACTCAGG AACCACTTAA GGCTTCTTGT 
AGTACAAACA TCAGTAGTCA TGAAAATCAG GAACTTTCTG AATCCCTGAA AGATAGTACC 
ACCAGCAAAA CTTTTGAGAA GAATGTTATA CGGCAGAGTA AAGAAAGCAT ATTGGAAAAG 
TTCTCAGTTC GAAAAGAAAT CATTAATTTG GAGAAAGAAA TGTTTAACGA GGGAACATGC 
ATTCAGCAAG ACAGTTTCTC ATCCAATGAC AGGGGGCCTT ATGAAACCTC AAAGCATGAA 
AAGCAACCTC CCGTGTATTG CACTTCTCCA GACTTTCAAA TGGGAGCTGC TTCTGATGCA 
TCTATGGCAA AATCCCCATT TAGTGCAGTA GGAGAAAGCA ATCTCCCTTC CCCATCACCT 
ACTGTATCTG TTAATCCTTT AACCAGAAGT CCCCCTGAAA CTTCTTCACA GTTGGCTCCT 
AATCCGTTAC TTTTAAGTCC TACCACAGAA CTAATGGAAG ACATTTCTGA ATCCGTTGGG 
AAGAACCAGT TTACTTCTGA AAGTACCCAC TTGAACATTG GTCATAGGTC TGTGGGTCAC 
AGCATGAATA TTGAATGTAA AGGGATTGAT AAAGAGCTAA CTGATTCAAA AACTGCACAT 
ATAGATATTC CAAGAATAAG CTCTTCCCTG GGAAAAAAGC CAAGTTTGAC TTCTGAATCC 
AGTATTCACA CAATTACCCC TTCAGTTGTT AACTTCACTA GTTTATTTAG TAACAAGCCG 
TTTCTAAAAC TTGGTGCAGT ATCTGCTTCT GACAAACACT GCCAGGTTGC TGAAAGCCTA 
AGCTCTAGTT TGCAGTCCAA ACCATTAAAA AAAAGGAAAG GAAGAAAAGC TCGGTGGACT 
AAAGTGGTGG CAAGAAGCAC ATGCCGGTCT CCAAAAGGGC TAGATTTAGA AAGATCAGAG 
CTTTTTAAAA GTGTTTCATG TAGCTCACTA TCAAATAGTA ATTCTGAGCC AGCCAAGTTC 
ATGAAAAACA TTGGACCATC TTCATTTGTA GATCATGACT TCCTTAAACG CCGATTGCCA 
AAGTTGAGCA AGTCCACAAC TCCATCTCTT GCTCTCTTAA CTGATAGTGA AAAACCATCT 
CACAAGGCTT TTGCTACTCA CAAATTATCC TCCAGTATGT GTGTCTCTAG TGACCTTTTG 
TCTGATATTT ATAAGCCCAA AAGAGGAAGA CCTAAAGCTA AAGAGATGCC TCAACTGGAA 
GGTCCACCTA AAAGGACTTT AAAAATCCCT GCCTCTAAAG TTTTTTCTTT GCAGTCTAAG 
GAAGAACAAG AACCCCCAAT TTTACAGCCA GAAATTGAAA TTCCTTCCTT CAAACAAAGT 
CTTTCTGTAT CTCCTTTTCC AAAAAAGCGA GGCAGACCTA AGAGGCAGTT GAGGTCACCG 
GTCAAGATGA AGCCACCTGT ACTATCAGTG GCTCCATTTG TTGCCACGGA GAGTCCAAGC 
AAGCTGGAGT CTGAAAGTGA TAACCATCGA AGCAGCAGTG ATTTCTTTGA GAGTGAGGAT 
CAGCTTCAGG ACCCAGATGA TCTGGACGAC AGTCATAGGC CGGCTGTCTG CAGCATGAGT 
GACCTTGAGA TGGAACCAGA TAAAAAAATT ACAAAGAGAA ACAATGGACA ATTAATGAAA 
ACAATTATCC GCAAAATAAA TAAAATGAAG ACTTTAAAAA GGAAGAAACT GTTGAATCAG 
ATTCTTTCAA GCTCTGTAGA ATCAAGTAAT AAAGGGAAAG TGCAATCCAA ACTCCATAAT 
ACAGTGTCAA GTCTTGCTGC CACGTTTGGC TCTAAATTGG GCCAACAGAT AAATGTCAGC 
AAGAAAGGAA CCATTTACAT AGGAAAGAGA AGGGGTCGAA AACCAAAAAC TGTCTTAAAT 
GGCATTCTTT CTGGTAGCCC TACCAGCCTT GCTGTCCTTG AGCAAACTGC TCAACAGGCT 
GCCGGCTCAG CATTGGGACA GATACTTCCC CCTTTACTGC CTTCATCTGC TAGTAGCTCA 
GAGATTCTCC CATCGCCTGT TTGCTCTCAG TCTTCTGGGA CTAGTGGTGG TCAGAGCCCT 
GTAAGTAGTG ATGCAGGCTT TGTTGAACCC AGCTCAGTGC CATATTTGCA TTTACACTCC 
AGACAGGGCA GTATGATTCA GACTCTTGCG ATGAAAAAGG CCTCAAAGGG GAGGAGGCGA 
CTTTCTCCTC CTACTTTGTT GCCAAATTCT CCTTCACACT TGAGTGAACT CACCTCTCTG 
AAAGAAGCTA CTCCTTCTCC CATTAGTGAG TCTCATAGTG ATGAGACCAT TCCCAGTGAT 
AGTGGAATTG GAACAGATAA TAACAGCACA TCGGACAGAG CAGAGAAATT TTGTGGACAG 
AAAAAGAGGA GGCACTCTTT TGAACATGTT TCTCTGATTC CCCCTGAAAC CTCTACAGTC 
CTGAGCAGTC TTAAAGAAAA ACATAAACAT AAATGTAAGC GCCGGAATCA CGATTACCTC 
AGCTATGACA AGATGAAAAG GCAGAAACGA AAACGGAAAA AGAAATACCC CCAGCTCCGT 
AGCAGACAGG ATCCAGACTT TATTGCGGAT CTGGAGGAAT TGATAAGTCG GCTGAGTGAA 
ATTCGAATCA CCCATCGAAG CCATCATTTT ATCCCCCGAG ACCTGCTGCC AACTATTTTT 
CGAATCAACT TTAATAGTTT CTATACACAT CCTTCTTTCC CCTTAGACCC CTTGCACTAC 
ATTCGAAAAC CTGACTTAAA AAAGAAGAGA GGGAGACCTC CTAAGATGAG GGAGGCAATG 
GCTGAGATGC CCTTCATGCA CAGCCTTAGT TTTCCTCTTT CTAGTACTGG ATTCTATCCC 
TCCTATGGCA TGCCTTACTC TCCCTCACCC CTTACAGCCA CTCCCCTAGG ATTAGGTTAC 
TATGGAAGGT ACCCTCCCAC TCTCTATCCT CCTCCTCCTT CACCTTCTTT CACCCCCCCA 
CTTCCTCCTC CTTCCTATAT GCATGCTGGC CATTTACTTC TCAATCCAAC CAAATACCAT 
AAGAAAAAGC ATAAGCTACT TCGCCAGGAG GCCTTTCTAA CAACCAGCAG GACTCCCCTC 
CTTTCCATGA GTACCTACCC TAGTGTTCCT CCTGAGATGG CCTATGGTTG GATGGTTGAG 
CACAAACATA GGCATCGTCA CAAGCACAGA GAACACCGTT CTTCTGAGCA ACCCCAGGTT 
TCCATGGATG CTGGCTCTTC CAGATCTGTT TTGGAGTCTT TGAAGCGCTA TCGATTTGGA 
AAGGAAACTG TTGGAGAGCG ATATAAACAT AAGGAAAAGC ACCGATGTCA TATGTCCTGC 
CCTCATCTCT CTCCTTCGAA AAGCTTAATA AATAGAGAAG AACAATGGGT CCACCGAGAG 
CCTTCAGAAT CTAGTCCATT GGCTTTGGGA TTGCAAACAC CTTTACAGAT TGACTGTTCC 
GAAAGTTCTC CAAGTTTATC TCTTGGAGGA TTCACTCCCA ACTCTGATCC GGCCAGCAGT 
GATGAACATA CAAACCTTTT CACAAGTGCA ATAGTGCAAG GCAGCTGCAG AGTTTCAAAC 
CCTAACTCCA GTGGCCGGAA GAAATTAACT GATAGCCCTG GACTGTTTTC TGCACAGGAC 
ACTTCACTAA TTCGGCCTCA CAGAAAGGAG CCACTGCCTT CCAGCGAAAG GGCAGTACAG 
ACCATGACAG GTTCCCAGCC AACCTCAGAT AAGTCTTCCC AGCGACCATC AGAGAGCACA 
AACTGTAGCC CTACCCGGAA GAGGTCTTCA TCTGAGAGTA CTTCTTCAAC AGTAAACGGA 
CTTCCTTCTC GAAGTCCAAG ATTGCTTGCC CCTGGGGATG ACTCTGTGGA TAGTCTGCTG 
CAGCGGATGG TACAACATGA GGACCAAGAG CCCCTGGAGA AGAATCTAGA TGCCGTGATT 
GCATCTGCCT CGGGGCCACC TTCTTCCAGT CCAGGCCACA GCCACAGCAA GGAACGAGCC 
CTGGGAAAAC CAGATAGCCT TCTAGTGCCC GCAGCCCCCA GTGACTCTTG TAGCAGCAGC 
ATCTCACTCC TGTCTGAGAA GTTGCCGAGC AGCTGCTCAC CCCACCATAT CAAGAGGAGT 
GTCGTGGAAG CCATGCAGCG CCAAGCTCGG AAAATGTGCA ATTATGACAA AATCTTGGCC 
ACAAAGAAAA ACCTGGACCA TGTCAATAAA ATATTAAAAG CCAAAAAGCT TCAAAGACAG 
GCCAGGACAG GGAATAACTT CGTGAAACGC AGACCAGGGC GACCTCGGAA ATGCCCCCTC 
CAGGCAGTGG TGTCAATGCA AGCATTCCAG GCTGCCCGAT TCGTCAGCCC CGATGCTAAT 
GAAGGTGAAG AAAGAGCAGC CCTGCCCCTC CGTCCAGACA CAGTTACGGA TGTCATCGAG 
GCTGTTGTGC AGAGCGTAAA CCTGAGTCCA GAACATAAGA AGGGGCTGAA GAGGAAAAGT 
TGGCTGTTGG AAGAACAGAC CAAGAAAAAG CAGAAGCCAT TCCCAGAGGA AGAACAAGAG 
CATCCCAAGA GCTTTACTGA AGCAGCAGTT GAGATTCCCA GTCCTCCTGA AACCCCAGCC 
AAGCCTTCCG AACCTGAAAG TACCTTGCAG CCTGTGCTTT CTCTCATCCC AAGGGAAAAG 
AAGGCCCCAC GTCCCCCAAA GAAGAAGTAT CAGAAAGCAG GGCTGTATTC TGACGTTTAC 
AAAACTACAG ACCCAAAGAG CCGATTGATC CAATTAAAGA AAGAAAAGTT GGAATACACT 
CCAGGAGAGC ATGAGTATGG ATTATTCCCA GCCCCTATTC ATGTTGGAAA GTATCTAAGG 
CAAAAGAGAA TTGACTTCCA GCTTCCCTAT GACATCCTTT GGCAGTGGAA ACATAATCAG 
CTATACAAAA AGCCAGATGT CCCACTATAT AAAAAAATCC GTTCAAACGT CTACGTGGAT 
GTCAAACCCC TTTCTGGTTA CGAGGCCACC ACCTGTAACT GTAAGAAGCC AGAGGATGAC 
ACCGGGAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCT 
AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGAGGCA TGAGTGGGTG 
CAGTGTCTAG AACGATTTCG AGCTGAGGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC 
CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGGGAGG TTGTCAGCGA ACAGGAGTTC 
AGGAACAGAA TGATTGAGCA GTATCATAAT CACAGTGACC ACTATTGCCT GAACCTGGAT 
AGTGGGATGG TGATTGACAG TTATCGGATG GGTAATGAGG CCCGATTCAT CAACCACAGC 
TGTGACCCAA ACTGTGAAAT GCAGAAATGG TCTGTCAATG GAGTATACCG GATTGGACTG 
TATGCTCTTA AAGATATGCC AGCTGGTACC GAGCTCACTT ATGATTACAA TTTCCATTCC 
TTTAATGTTG AGAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT 
GGGGGCAAGA GTCAGCGCGT GAACGGACTC GCCAGCAGCA AAAGCAGCCA GCCTGTGGCT 
ACCCATAAAA AGTCTGGACG GTCAAAAGAG AAGAGAAAGT CAAAGCACAA GCTGAAGAAA 
AGGAGAGGCC ACCTCTCCGA AGAACCCAGT GAAAACATCA ACACTCCAAC AAGATTGACC 
CCCCAATTAC AAATGAAGCC AATGTCTAAT CGAGAGAGGA ACTTCGTGTT AAAGCATCAT 
GTATTCTTGG TCCGAAACTG GGAGAAGATC CGACAGAAAC AAGAGGAAGT AAAGCATACC 
AGCGATAATC TCCACTCTGC ATCATTATAC ACCCGCTGGA ATGGCATCTG CCGAGATGAT 
GGGAACATCA AGTCTGACGT CTTCATGACC CAGTTCTCCG CCCTGCAGAC AGCACGATCA 
GTTCGAACAA GACGCCTGGC AGCAGCAGAG GAAAACATCG AGGTGGCACG GGCAGCCCGC 
CTCGCCCAAA TCTTCAAAGA GATCTGTGAT GGCATCATCT CCTACAAAGA TTCTTCCCGA 
CAAGCTTTGG CAGCACCCCT CTTGAACCTC CCCCCAAAGA AAAAGAATGC TGATTACTAT 
GAGAAGATCT CAGAGCCCCT GGACTTGGCC ACCATAGAGA GGCAGATCCT CACCGGCCAT 
TACAAGACCG TGGAGGCCTT TGACGCTGAC ATGCTCAAGG TGTTTCGGAA TGCTGAGAAG 
TACTACGGGC GTAAATCCCC AATTGGGAGA GATGTTTGCC GTCTACGGAA GACCTACTAC 
AACGCCAGGC ATGAGGCATC AGCCCAGATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG 
GCCGACAGCA GCGAAACATC TGTCTCTGAG AAGGAGAACG TGCATGAGAA GGATGACGAT 
GTGATCCGCT GTGTCTGTGG CCTCCACAAG GATGAGGGCC TCATGATCCA GTGTGACAAG 
TGCATGGTAT GGCAGCACTG TGACTGTATG GGGGTGAACT CAGACGTGGA GCATTACCTG 
TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC 
TATGCCCAGC CCGGCTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GTTGCTTCGT 
CAAGGTGACT GTGTGTACCT GATGCGAGAT AGCCGGCGCA CCCCTGATGG CCACCCAGTC 
CGCCAGTCCT ACCGACTGTT ATCACACATT AACCGAGATA AACTTGACAT CTTCCGCATT 
GAGAAGCTCT GGAAGAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA CTACTTCCGC 
CCCCACGAGA CCCACCACTC TCCGTCACGC CGCTTCTATC ACAATGAGCT ATTTAGGGTA 
CCACTCTATG AGATCATTCC CTTGGAGGCT GTGGTGGGGA CCTGCTGTGT ACTAGACCTT 
TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTATA CATCTGTGAC 
TATCGGCTTG ACAAGTCCGC ACACCTGTTT TACAAGATTC ACCGGAATCG CTATCCTGTC 
TGCACCAAAC CCTACGCCTT TGACCACTTC CCCAAGAAGC TCACTCCTAA AAGAGATTTC 
TCACCTCATT ATGTCCCAGA CAACTACAAG AGGAACGGAG GGCGGTCATC CTGGAAGTCT 
GAGCGCCCAA AGCCACCTCT AAAAGACCTG GGCCAGGAGG ATGACGCTCT GCCCTTGATT 
GAAGAGGTTC TGGCCAGCCA GGAGCAAGCC ACCAATGAGC TGCCCAGCCT GGAGGAGCCA 
GAACGGGAAG TGGCCCCTGT GGAAGTCAGC GAGGGTGAAA AGAAAGCAGA GGAAGGTGGT 
CCAGAATCTC AGGCTGCCTG TACCCCTGAG GAGCGACGGC ACAGCCAACG GGAACGGCTC 
AACCAGATCC TACTTGGCCT CCTGGAAAAA ATCCCTGGGA AAAATGCCAT CGATGTGACC 
TACTTGCTGG AGGAAGGGTC AGGCAGGAAG CTTCGAAGGC GCACTTTGTT CATCCCAGAG 
AACAGCTTTC GGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970813.1
CDS	160..9057
Translation	MDPRNTAMLGLGSDSEGFSRKSPSALGTGTVVSKREGELESNTKEEEDLRKRNWDRNSEAGKEDGLTDAQQQFSVKETNFSEGNLKLKIGLQAKRTKKPPKNLENYVCRPAIKTTIKHPRKALKGGKMTEEKDEHCPSRRDPSKLYKKTGDVAAVECQSEESIHLHSQGENSPLSKKLSPGHSEMTDYMNAASSTLVGSRDPDLKDRALLNGGTSVTEKLAQLIATCPPSKSSKTKPKKLGSGTSAGLVSKDLIRKAGVGSVAGIIHKDLIKKPAISTAVGLVTKDPGKKPVFNATVGLVNKDSVKKLGAGTTAVFINKDLGRKPGTVTTVGLLSKDSGKKLGIGIVPGLVNKESGKKLGLGTVVGLVNKDLGKKLSSTVGLVAKDCAKKIVANSTMGLVTKDMGKKLVSCSMAGLVNKDAINLKAEALLPTQEPLKASCSTNISSHENQELSESLKDSTTSKTFEKNVIRQSKESILEKFSVRKEIINLEKEMFNEGTCIQQDSFSSNDRGPYETSKHEKQPPVYCTSPDFQMGAASDASMAKSPFSAVGESNLPSPSPTVSVNPLTRSPPETSSQLAPNPLLLSPTTELMEDISESVGKNQFTSESTHLNIGHRSVGHSMNIECKGIDKELTDSKTAHIDIPRISSSLGKKPSLTSESSIHTITPSVVNFTSLFSNKPFLKLGAVSASDKHCQVAESLSSSLQSKPLKKRKGRKARWTKVVARSTCRSPKGLDLERSELFKSVSCSSLSNSNSEPAKFMKNIGPSSFVDHDFLKRRLPKLSKSTTPSLALLTDSEKPSHKAFATHKLSSSMCVSSDLLSDIYKPKRGRPKAKEMPQLEGPPKRTLKIPASKVFSLQSKEEQEPPILQPEIEIPSFKQSLSVSPFPKKRGRPKRQLRSPVKMKPPVLSVAPFVATESPSKLESESDNHRSSSDFFESEDQLQDPDDLDDSHRPAVCSMSDLEMEPDKKITKRNNGQLMKTIIRKINKMKTLKRKKLLNQILSSSVESSNKGKVQSKLHNTVSSLAATFGSKLGQQINVSKKGTIYIGKRRGRKPKTVLNGILSGSPTSLAVLEQTAQQAAGSALGQILPPLLPSSASSSEILPSPVCSQSSGTSGGQSPVSSDAGFVEPSSVPYLHLHSRQGSMIQTLAMKKASKGRRRLSPPTLLPNSPSHLSELTSLKEATPSPISESHSDETIPSDSGIGTDNNSTSDRAEKFCGQKKRRHSFEHVSLIPPETSTVLSSLKEKHKHKCKRRNHDYLSYDKMKRQKRKRKKKYPQLRSRQDPDFIADLEELISRLSEIRITHRSHHFIPRDLLPTIFRINFNSFYTHPSFPLDPLHYIRKPDLKKKRGRPPKMREAMAEMPFMHSLSFPLSSTGFYPSYGMPYSPSPLTATPLGLGYYGRYPPTLYPPPPSPSFTPPLPPPSYMHAGHLLLNPTKYHKKKHKLLRQEAFLTTSRTPLLSMSTYPSVPPEMAYGWMVEHKHRHRHKHREHRSSEQPQVSMDAGSSRSVLESLKRYRFGKETVGERYKHKEKHRCHMSCPHLSPSKSLINREEQWVHREPSESSPLALGLQTPLQIDCSESSPSLSLGGFTPNSDPASSDEHTNLFTSAIVQGSCRVSNPNSSGRKKLTDSPGLFSAQDTSLIRPHRKEPLPSSERAVQTMTGSQPTSDKSSQRPSESTNCSPTRKRSSSESTSSTVNGLPSRSPRLLAPGDDSVDSLLQRMVQHEDQEPLEKNLDAVIASASGPPSSSPGHSHSKERALGKPDSLLVPAAPSDSCSSSISLLSEKLPSSCSPHHIKRSVVEAMQRQARKMCNYDKILATKKNLDHVNKILKAKKLQRQARTGNNFVKRRPGRPRKCPLQAVVSMQAFQAARFVSPDANEGEERAALPLRPDTVTDVIEAVVQSVNLSPEHKKGLKRKSWLLEEQTKKKQKPFPEEEQEHPKSFTEAAVEIPSPPETPAKPSEPESTLQPVLSLIPREKKAPRPPKKKYQKAGLYSDVYKTTDPKSRLIQLKKEKLEYTPGEHEYGLFPAPIHVGKYLRQKRIDFQLPYDILWQWKHNQLYKKPDVPLYKKIRSNVYVDVKPLSGYEATTCNCKKPEDDTGKGCVDDCLNRMIFAECSPNTCPCGEQCCNQRIQRHEWVQCLERFRAEEKGWGIRTKEPLKAGQFIIEYLGEVVSEQEFRNRMIEQYHNHSDHYCLNLDSGMVIDSYRMGNEARFINHSCDPNCEMQKWSVNGVYRIGLYALKDMPAGTELTYDYNFHSFNVEKQQLCKCGFEKCRGIIGGKSQRVNGLASSKSSQPVATHKKSGRSKEKRKSKHKLKKRRGHLSEEPSENINTPTRLTPQLQMKPMSNRERNFVLKHHVFLVRNWEKIRQKQEEVKHTSDNLHSASLYTRWNGICRDDGNIKSDVFMTQFSALQTARSVRTRRLAAAEENIEVARAARLAQIFKEICDGIISYKDSSRQALAAPLLNLPPKKKNADYYEKISEPLDLATIERQILTGHYKTVEAFDADMLKVFRNAEKYYGRKSPIGRDVCRLRKTYYNARHEASAQIDEIVGETASEADSSETSVSEKENVHEKDDDVIRCVCGLHKDEGLMIQCDKCMVWQHCDCMGVNSDVEHYLCEQCDPRPVDREVPMIPRPHYAQPGCVYFICLLRDDLLLRQGDCVYLMRDSRRTPDGHPVRQSYRLLSHINRDKLDIFRIEKLWKNEKEERFAFGHHYFRPHETHHSPSRRFYHNELFRVPLYEIIPLEAVVGTCCVLDLYTYCKGRPKGVKEQDVYICDYRLDKSAHLFYKIHRNRYPVCTKPYAFDHFPKKLTPKRDFSPHYVPDNYKRNGGRSSWKSERPKPPLKDLGQEDDALPLIEEVLASQEQATNELPSLEEPEREVAPVEVSEGEKKAEEGGPESQAACTPEERRHSQRERLNQILLGLLEKIPGKNAIDVTYLLEEGSGRKLRRRTLFIPENSFRK

Target ORF information:

RefSeq Version	XM_014115338.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115338.2

ORF Insert Sequence:

ATGGACCCTA GAAATACTGC TATGTTAGGA TTGGGTTCTG ACTCGGAAGG ATTTTCAAGG 
AAGAGTCCCT CTGCCCTTGG TACCGGCACA GTGGTCAGCA AGAGAGAAGG AGAGCTAGAG 
AGCAACACGA AAGAGGAAGA GGACCTTCGC AAGAGGAATT GGGACAGAAA CAGCGAAGCA 
GGGAAGGAGG ATGGTTTGAC TGATGCACAG CAACAGTTTT CAGTGAAAGA AACAAACTTT 
TCAGAGGGAA ACTTAAAATT GAAAATTGGC CTTCAGGCTA AGAGAACTAA AAAACCTCCA 
AAAAACTTGG AGAACTATGT ATGTCGACCT GCCATAAAAA CAACTATCAA GCACCCAAGG 
AAAGCACTTA AAGGTGGGAA GATGACAGAG GAGAAGGATG AGCACTGTCC TTCAAGACGG 
GACCCTTCAA AGTTGTACAA GAAAACAGGT GATGTTGCAG CCGTTGAATG CCAATCTGAA 
GAGAGCATCC ATCTTCATTC ACAGGGAGAA AACAGTCCTT TGTCTAAGAA GCTTTCTCCA 
GGACACTCAG AGATGACAGA TTACATGAAT GCAGCATCAT CTACTCTTGT TGGTAGCCGG 
GATCCTGATT TAAAGGATAG AGCATTACTT AATGGAGGAA CAAGTGTAAC AGAAAAGTTG 
GCACAGCTTA TTGCAACCTG TCCTCCCTCT AAGTCTTCCA AGACTAAACC GAAGAAGTTA 
GGAAGTGGCA CCAGCGCAGG ATTGGTTAGT AAGGATTTGA TCAGGAAAGC AGGTGTTGGC 
TCTGTAGCTG GAATAATACA TAAAGATTTA ATTAAAAAAC CAGCTATCAG CACAGCAGTT 
GGATTGGTAA CTAAAGATCC TGGGAAAAAG CCAGTGTTTA ATGCAACAGT AGGATTGGTC 
AATAAGGACT CTGTGAAAAA ACTAGGAGCT GGCACTACAG CGGTATTCAT TAATAAAGAC 
TTAGGCAGAA AGCCAGGGAC TGTCACTACA GTAGGACTGC TGAGCAAGGA TTCAGGAAAG 
AAGCTAGGAA TTGGTATTGT TCCAGGTTTA GTGAATAAGG AATCTGGAAA GAAGTTAGGA 
CTCGGCACTG TGGTCGGACT GGTTAATAAA GACTTGGGCA AGAAGTTGAG TTCTACTGTC 
GGTCTAGTGG CCAAGGACTG TGCAAAGAAG ATTGTAGCCA ATTCAACAAT GGGATTAGTT 
ACTAAAGACA TGGGAAAGAA ACTAGTGAGT TGTTCTATGG CAGGATTGGT CAATAAAGAT 
GCCATAAACC TTAAAGCCGA AGCACTGCTC CCCACTCAGG AACCACTTAA GGCTTCTTGT 
AGTACAAACA TCAGTAGTCA TGAAAATCAG GAACTTTCTG AATCCCTGAA AGATAGTACC 
ACCAGCAAAA CTTTTGAGAA GAATGTTATA CGGCAGAGTA AAGAAAGCAT ATTGGAAAAG 
TTCTCAGTTC GAAAAGAAAT CATTAATTTG GAGAAAGAAA TGTTTAACGA GGGAACATGC 
ATTCAGCAAG ACAGTTTCTC ATCCAATGAC AGGGGGCCTT ATGAAACCTC AAAGCATGAA 
AAGCAACCTC CCGTGTATTG CACTTCTCCA GACTTTCAAA TGGGAGCTGC TTCTGATGCA 
TCTATGGCAA AATCCCCATT TAGTGCAGTA GGAGAAAGCA ATCTCCCTTC CCCATCACCT 
ACTGTATCTG TTAATCCTTT AACCAGAAGT CCCCCTGAAA CTTCTTCACA GTTGGCTCCT 
AATCCGTTAC TTTTAAGTCC TACCACAGAA CTAATGGAAG ACATTTCTGA ATCCGTTGGG 
AAGAACCAGT TTACTTCTGA AAGTACCCAC TTGAACATTG GTCATAGGTC TGTGGGTCAC 
AGCATGAATA TTGAATGTAA AGGGATTGAT AAAGAGCTAA CTGATTCAAA AACTGCACAT 
ATAGATATTC CAAGAATAAG CTCTTCCCTG GGAAAAAAGC CAAGTTTGAC TTCTGAATCC 
AGTATTCACA CAATTACCCC TTCAGTTGTT AACTTCACTA GTTTATTTAG TAACAAGCCG 
TTTCTAAAAC TTGGTGCAGT ATCTGCTTCT GACAAACACT GCCAGGTTGC TGAAAGCCTA 
AGCTCTAGTT TGCAGTCCAA ACCATTAAAA AAAAGGAAAG GAAGAAAAGC TCGGTGGACT 
AAAGTGGTGG CAAGAAGCAC ATGCCGGTCT CCAAAAGGGC TAGATTTAGA AAGATCAGAG 
CTTTTTAAAA GTGTTTCATG TAGCTCACTA TCAAATAGTA ATTCTGAGCC AGCCAAGTTC 
ATGAAAAACA TTGGACCATC TTCATTTGTA GATCATGACT TCCTTAAACG CCGATTGCCA 
AAGTTGAGCA AGTCCACAAC TCCATCTCTT GCTCTCTTAA CTGATAGTGA AAAACCATCT 
CACAAGGCTT TTGCTACTCA CAAATTATCC TCCAGTATGT GTGTCTCTAG TGACCTTTTG 
TCTGATATTT ATAAGCCCAA AAGAGGAAGA CCTAAAGCTA AAGAGATGCC TCAACTGGAA 
GGTCCACCTA AAAGGACTTT AAAAATCCCT GCCTCTAAAG TTTTTTCTTT GCAGTCTAAG 
GAAGAACAAG AACCCCCAAT TTTACAGCCA GAAATTGAAA TTCCTTCCTT CAAACAAAGT 
CTTTCTGTAT CTCCTTTTCC AAAAAAGCGA GGCAGACCTA AGAGGCAGTT GAGGTCACCG 
GTCAAGATGA AGCCACCTGT ACTATCAGTG GCTCCATTTG TTGCCACGGA GAGTCCAAGC 
AAGCTGGAGT CTGAAAGTGA TAACCATCGA AGCAGCAGTG ATTTCTTTGA GAGTGAGGAT 
CAGCTTCAGG ACCCAGATGA TCTGGACGAC AGTCATAGGC CGGCTGTCTG CAGCATGAGT 
GACCTTGAGA TGGAACCAGA TAAAAAAATT ACAAAGAGAA ACAATGGACA ATTAATGAAA 
ACAATTATCC GCAAAATAAA TAAAATGAAG ACTTTAAAAA GGAAGAAACT GTTGAATCAG 
ATTCTTTCAA GCTCTGTAGA ATCAAGTAAT AAAGGGAAAG TGCAATCCAA ACTCCATAAT 
ACAGTGTCAA GTCTTGCTGC CACGTTTGGC TCTAAATTGG GCCAACAGAT AAATGTCAGC 
AAGAAAGGAA CCATTTACAT AGGAAAGAGA AGGGGTCGAA AACCAAAAAC TGTCTTAAAT 
GGCATTCTTT CTGGTAGCCC TACCAGCCTT GCTGTCCTTG AGCAAACTGC TCAACAGGCT 
GCCGGCTCAG CATTGGGACA GATACTTCCC CCTTTACTGC CTTCATCTGC TAGTAGCTCA 
GAGATTCTCC CATCGCCTGT TTGCTCTCAG TCTTCTGGGA CTAGTGGTGG TCAGAGCCCT 
GTAAGTAGTG ATGCAGGCTT TGTTGAACCC AGCTCAGTGC CATATTTGCA TTTACACTCC 
AGACAGGGCA GTATGATTCA GACTCTTGCG ATGAAAAAGG CCTCAAAGGG GAGGAGGCGA 
CTTTCTCCTC CTACTTTGTT GCCAAATTCT CCTTCACACT TGAGTGAACT CACCTCTCTG 
AAAGAAGCTA CTCCTTCTCC CATTAGTGAG TCTCATAGTG ATGAGACCAT TCCCAGTGAT 
AGTGGAATTG GAACAGATAA TAACAGCACA TCGGACAGAG CAGAGAAATT TTGTGGACAG 
AAAAAGAGGA GGCACTCTTT TGAACATGTT TCTCTGATTC CCCCTGAAAC CTCTACAGTC 
CTGAGCAGTC TTAAAGAAAA ACATAAACAT AAATGTAAGC GCCGGAATCA CGATTACCTC 
AGCTATGACA AGATGAAAAG GCAGAAACGA AAACGGAAAA AGAAATACCC CCAGCTCCGT 
AGCAGACAGG ATCCAGACTT TATTGCGGAT CTGGAGGAAT TGATAAGTCG GCTGAGTGAA 
ATTCGAATCA CCCATCGAAG CCATCATTTT ATCCCCCGAG ACCTGCTGCC AACTATTTTT 
CGAATCAACT TTAATAGTTT CTATACACAT CCTTCTTTCC CCTTAGACCC CTTGCACTAC 
ATTCGAAAAC CTGACTTAAA AAAGAAGAGA GGGAGACCTC CTAAGATGAG GGAGGCAATG 
GCTGAGATGC CCTTCATGCA CAGCCTTAGT TTTCCTCTTT CTAGTACTGG ATTCTATCCC 
TCCTATGGCA TGCCTTACTC TCCCTCACCC CTTACAGCCA CTCCCCTAGG ATTAGGTTAC 
TATGGAAGGT ACCCTCCCAC TCTCTATCCT CCTCCTCCTT CACCTTCTTT CACCCCCCCA 
CTTCCTCCTC CTTCCTATAT GCATGCTGGC CATTTACTTC TCAATCCAAC CAAATACCAT 
AAGAAAAAGC ATAAGCTACT TCGCCAGGAG GCCTTTCTAA CAACCAGCAG GACTCCCCTC 
CTTTCCATGA GTACCTACCC TAGTGTTCCT CCTGAGATGG CCTATGGTTG GATGGTTGAG 
CACAAACATA GGCATCGTCA CAAGCACAGA GAACACCGTT CTTCTGAGCA ACCCCAGGTT 
TCCATGGATG CTGGCTCTTC CAGATCTGTT TTGGAGTCTT TGAAGCGCTA TCGATTTGGA 
AAGGAAACTG TTGGAGAGCG ATATAAACAT AAGGAAAAGC ACCGATGTCA TATGTCCTGC 
CCTCATCTCT CTCCTTCGAA AAGCTTAATA AATAGAGAAG AACAATGGGT CCACCGAGAG 
CCTTCAGAAT CTAGTCCATT GGCTTTGGGA TTGCAAACAC CTTTACAGAT TGACTGTTCC 
GAAAGTTCTC CAAGTTTATC TCTTGGAGGA TTCACTCCCA ACTCTGATCC GGCCAGCAGT 
GATGAACATA CAAACCTTTT CACAAGTGCA ATAGTGCAAG GCAGCTGCAG AGTTTCAAAC 
CCTAACTCCA GTGGCCGGAA GAAATTAACT GATAGCCCTG GACTGTTTTC TGCACAGGAC 
ACTTCACTAA TTCGGCCTCA CAGAAAGGAG CCACTGCCTT CCAGCGAAAG GGCAGTACAG 
ACCATGACAG GTTCCCAGCC AACCTCAGAT AAGTCTTCCC AGCGACCATC AGAGAGCACA 
AACTGTAGCC CTACCCGGAA GAGGTCTTCA TCTGAGAGTA CTTCTTCAAC AGTAAACGGA 
CTTCCTTCTC GAAGTCCAAG ATTGCTTGCC CCTGGGGATG ACTCTGTGGA TAGTCTGCTG 
CAGCGGATGG TACAACATGA GGACCAAGAG CCCCTGGAGA AGAATCTAGA TGCCGTGATT 
GCATCTGCCT CGGGGCCACC TTCTTCCAGT CCAGGCCACA GCCACAGCAA GGAACGAGCC 
CTGGGAAAAC CAGATAGCCT TCTAGTGCCC GCAGCCCCCA GTGACTCTTG TAGCAGCAGC 
ATCTCACTCC TGTCTGAGAA GTTGCCGAGC AGCTGCTCAC CCCACCATAT CAAGAGGAGT 
GTCGTGGAAG CCATGCAGCG CCAAGCTCGG AAAATGTGCA ATTATGACAA AATCTTGGCC 
ACAAAGAAAA ACCTGGACCA TGTCAATAAA ATATTAAAAG CCAAAAAGCT TCAAAGACAG 
GCCAGGACAG GGAATAACTT CGTGAAACGC AGACCAGGGC GACCTCGGAA ATGCCCCCTC 
CAGGCAGTGG TGTCAATGCA AGCATTCCAG GCTGCCCGAT TCGTCAGCCC CGATGCTAAT 
GAAGGTGAAG AAAGAGCAGC CCTGCCCCTC CGTCCAGACA CAGTTACGGA TGTCATCGAG 
GCTGTTGTGC AGAGCGTAAA CCTGAGTCCA GAACATAAGA AGGGGCTGAA GAGGAAAAGT 
TGGCTGTTGG AAGAACAGAC CAAGAAAAAG CAGAAGCCAT TCCCAGAGGA AGAACAAGAG 
CATCCCAAGA GCTTTACTGA AGCAGCAGTT GAGATTCCCA GTCCTCCTGA AACCCCAGCC 
AAGCCTTCCG AACCTGAAAG TACCTTGCAG CCTGTGCTTT CTCTCATCCC AAGGGAAAAG 
AAGGCCCCAC GTCCCCCAAA GAAGAAGTAT CAGAAAGCAG GGCTGTATTC TGACGTTTAC 
AAAACTACAG ACCCAAAGAG CCGATTGATC CAATTAAAGA AAGAAAAGTT GGAATACACT 
CCAGGAGAGC ATGAGTATGG ATTATTCCCA GCCCCTATTC ATGTTGGAAA GTATCTAAGG 
CAAAAGAGAA TTGACTTCCA GCTTCCCTAT GACATCCTTT GGCAGTGGAA ACATAATCAG 
CTATACAAAA AGCCAGATGT CCCACTATAT AAAAAAATCC GTTCAAACGT CTACGTGGAT 
GTCAAACCCC TTTCTGGTTA CGAGGCCACC ACCTGTAACT GTAAGAAGCC AGAGGATGAC 
ACCGGGAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCT 
AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGAGGCA TGAGTGGGTG 
CAGTGTCTAG AACGATTTCG AGCTGAGGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC 
CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGGGAGG TTGTCAGCGA ACAGGAGTTC 
AGGAACAGAA TGATTGAGCA GTATCATAAT CACAGTGACC ACTATTGCCT GAACCTGGAT 
AGTGGGATGG TGATTGACAG TTATCGGATG GGTAATGAGG CCCGATTCAT CAACCACAGC 
TGTGACCCAA ACTGTGAAAT GCAGAAATGG TCTGTCAATG GAGTATACCG GATTGGACTG 
TATGCTCTTA AAGATATGCC AGCTGGTACC GAGCTCACTT ATGATTACAA TTTCCATTCC 
TTTAATGTTG AGAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT 
GGGGGCAAGA GTCAGCGCGT GAACGGACTC GCCAGCAGCA AAAGCAGCCA GCCTGTGGCT 
ACCCATAAAA AGTCTGGACG GTCAAAAGAG AAGAGAAAGT CAAAGCACAA GCTGAAGAAA 
AGGAGAGGCC ACCTCTCCGA AGAACCCAGT GAAAACATCA ACACTCCAAC AAGATTGACC 
CCCCAATTAC AAATGAAGCC AATGTCTAAT CGAGAGAGGA ACTTCGTGTT AAAGCATCAT 
GTATTCTTGG TCCGAAACTG GGAGAAGATC CGACAGAAAC AAGAGGAAGT AAAGCATACC 
AGCGATAATC TCCACTCTGC ATCATTATAC ACCCGCTGGA ATGGCATCTG CCGAGATGAT 
GGGAACATCA AGTCTGACGT CTTCATGACC CAGTTCTCCG CCCTGCAGAC AGCACGATCA 
GTTCGAACAA GACGCCTGGC AGCAGCAGAG GAAAACATCG AGGTGGCACG GGCAGCCCGC 
CTCGCCCAAA TCTTCAAAGA GATCTGTGAT GGCATCATCT CCTACAAAGA TTCTTCCCGA 
CAAGCTTTGG CAGCACCCCT CTTGAACCTC CCCCCAAAGA AAAAGAATGC TGATTACTAT 
GAGAAGATCT CAGAGCCCCT GGACTTGGCC ACCATAGAGA GGCAGATCCT CACCGGCCAT 
TACAAGACCG TGGAGGCCTT TGACGCTGAC ATGCTCAAGG TGTTTCGGAA TGCTGAGAAG 
TACTACGGGC GTAAATCCCC AATTGGGAGA GATGTTTGCC GTCTACGGAA GACCTACTAC 
AACGCCAGGC ATGAGGCATC AGCCCAGATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG 
GCCGACAGCA GCGAAACATC TGTCTCTGAG AAGGAGAACG TGCATGAGAA GGATGACGAT 
GTGATCCGCT GTGTCTGTGG CCTCCACAAG GATGAGGGCC TCATGATCCA GTGTGACAAG 
TGCATGGTAT GGCAGCACTG TGACTGTATG GGGGTGAACT CAGACGTGGA GCATTACCTG 
TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC 
TATGCCCAGC CCGGCTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GTTGCTTCGT 
CAAGGTGACT GTGTGTACCT GATGCGAGAT AGCCGGCGCA CCCCTGATGG CCACCCAGTC 
CGCCAGTCCT ACCGACTGTT ATCACACATT AACCGAGATA AACTTGACAT CTTCCGCATT 
GAGAAGCTCT GGAAGAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA CTACTTCCGC 
CCCCACGAGA CCCACCACTC TCCGTCACGC CGCTTCTATC ACAATGAGCT ATTTAGGGTA 
CCACTCTATG AGATCATTCC CTTGGAGGCT GTGGTGGGGA CCTGCTGTGT ACTAGACCTT 
TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTATA CATCTGTGAC 
TATCGGCTTG ACAAGTCCGC ACACCTGTTT TACAAGATTC ACCGGAATCG CTATCCTGTC 
TGCACCAAAC CCTACGCCTT TGACCACTTC CCCAAGAAGC TCACTCCTAA AAGAGATTTC 
TCACCTCATT ATGTCCCAGA CAACTACAAG AGGAACGGAG GGCGGTCATC CTGGAAGTCT 
GAGCGCCCAA AGCCACCTCT AAAAGACCTG GGCCAGGAGG ATGACGCTCT GCCCTTGATT 
GAAGAGGTTC TGGCCAGCCA GGAGCAAGCC ACCAATGAGC TGCCCAGCCT GGAGGAGCCA 
GAACGGGAAG TGGCCCCTGT GGAAGTCAGC GAGGGTGAAA AGAAAGCAGA GGAAGGTGGT 
CCAGAATCTC AGGCTGCCTG TACCCCTGAG GAGCGACGGC ACAGCCAACG GGAACGGCTC 
AACCAGATCC TACTTGGCCT CCTGGAAAAA ATCCCTGGGA AAAATGCCAT CGATGTGACC 
TACTTGCTGG AGGAAGGGTC AGGCAGGAAG CTTCGAAGGC GCACTTTGTT CATCCCAGAG 
AACAGCTTTC GGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASH1L ( XM_537251.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_537251.2 Canis lupus familiaris ash1 (absent, small, or homeotic)-like (Drosophila) (ASH1L), transcript variant X1, mRNA.

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CloneID	OCe09533
Clone ID Related Accession (Same CDS sequence)	XM_014115336.2 , XM_537251.6 , XM_014115338.2 , XM_537251.5 , XM_014115337.1 , XM_014115338.1 , XM_014115336.1 , XM_014115337.2
Accession Version	XM_537251.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8898bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	histone-lysine N-methyltransferase ASH1L
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_537251.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCTA GAAATACTGC TATGTTAGGA TTGGGTTCTG ACTCGGAAGG ATTTTCAAGG 
AAGAGTCCCT CTGCCCTTGG TACCGGCACA GTGGTCAGCA AGAGAGAAGG AGAGCTAGAG 
AGCAACACGA AAGAGGAAGA GGACCTTCGC AAGAGGAATT GGGACAGAAA CAGCGAAGCA 
GGGAAGGAGG ATGGTTTGAC TGATGCACAG CAACAGTTTT CAGTGAAAGA AACAAACTTT 
TCAGAGGGAA ACTTAAAATT GAAAATTGGC CTTCAGGCTA AGAGAACTAA AAAACCTCCA 
AAAAACTTGG AGAACTATGT ATGTCGACCT GCCATAAAAA CAACTATCAA GCACCCAAGG 
AAAGCACTTA AAGGTGGGAA GATGACAGAG GAGAAGGATG AGCACTGTCC TTCAAGACGG 
GACCCTTCAA AGTTGTACAA GAAAACAGGT GATGTTGCAG CCGTTGAATG CCAATCTGAA 
GAGAGCATCC ATCTTCATTC ACAGGGAGAA AACAGTCCTT TGTCTAAGAA GCTTTCTCCA 
GGACACTCAG AGATGACAGA TTACATGAAT GCAGCATCAT CTACTCTTGT TGGTAGCCGG 
GATCCTGATT TAAAGGATAG AGCATTACTT AATGGAGGAA CAAGTGTAAC AGAAAAGTTG 
GCACAGCTTA TTGCAACCTG TCCTCCCTCT AAGTCTTCCA AGACTAAACC GAAGAAGTTA 
GGAAGTGGCA CCAGCGCAGG ATTGGTTAGT AAGGATTTGA TCAGGAAAGC AGGTGTTGGC 
TCTGTAGCTG GAATAATACA TAAAGATTTA ATTAAAAAAC CAGCTATCAG CACAGCAGTT 
GGATTGGTAA CTAAAGATCC TGGGAAAAAG CCAGTGTTTA ATGCAACAGT AGGATTGGTC 
AATAAGGACT CTGTGAAAAA ACTAGGAGCT GGCACTACAG CGGTATTCAT TAATAAAGAC 
TTAGGCAGAA AGCCAGGGAC TGTCACTACA GTAGGACTGC TGAGCAAGGA TTCAGGAAAG 
AAGCTAGGAA TTGGTATTGT TCCAGGTTTA GTGAATAAGG AATCTGGAAA GAAGTTAGGA 
CTCGGCACTG TGGTCGGACT GGTTAATAAA GACTTGGGCA AGAAGTTGAG TTCTACTGTC 
GGTCTAGTGG CCAAGGACTG TGCAAAGAAG ATTGTAGCCA ATTCAACAAT GGGATTAGTT 
ACTAAAGACA TGGGAAAGAA ACTAGTGAGT TGTTCTATGG CAGGATTGGT CAATAAAGAT 
GCCATAAACC TTAAAGCCGA AGCACTGCTC CCCACTCAGG AACCACTTAA GGCTTCTTGT 
AGTACAAACA TCAGTAGTCA TGAAAATCAG GAACTTTCTG AATCCCTGAA AGATAGTACC 
ACCAGCAAAA CTTTTGAGAA GAATGTTATA CGGCAGAGTA AAGAAAGCAT ATTGGAAAAG 
TTCTCAGTTC GAAAAGAAAT CATTAATTTG GAGAAAGAAA TGTTTAACGA GGGAACATGC 
ATTCAGCAAG ACAGTTTCTC ATCCAATGAC AGGGGGCCTT ATGAAACCTC AAAGCATGAA 
AAGCAACCTC CCGTGTATTG CACTTCTCCA GACTTTCAAA TGGGAGCTGC TTCTGATGCA 
TCTATGGCAA AATCCCCATT TAGTGCAGTA GGAGAAAGCA ATCTCCCTTC CCCATCACCT 
ACTGTATCTG TTAATCCTTT AACCAGAAGT CCCCCTGAAA CTTCTTCACA GTTGGCTCCT 
AATCCGTTAC TTTTAAGTCC TACCACAGAA CTAATGGAAG ACATTTCTGA ATCCGTTGGG 
AAGAACCAGT TTACTTCTGA AAGTACCCAC TTGAACATTG GTCATAGGTC TGTGGGTCAC 
AGCATGAATA TTGAATGTAA AGGGATTGAT AAAGAGCTAA CTGATTCAAA AACTGCACAT 
ATAGATATTC CAAGAATAAG CTCTTCCCTG GGAAAAAAGC CAAGTTTGAC TTCTGAATCC 
AGTATTCACA CAATTACCCC TTCAGTTGTT AACTTCACTA GTTTATTTAG TAACAAGCCG 
TTTCTAAAAC TTGGTGCAGT ATCTGCTTCT GACAAACACT GCCAGGTTGC TGAAAGCCTA 
AGCTCTAGTT TGCAGTCCAA ACCATTAAAA AAAAGGAAAG GAAGAAAAGC TCGGTGGACT 
AAAGTGGTGG CAAGAAGCAC ATGCCGGTCT CCAAAAGGGC TAGATTTAGA AAGATCAGAG 
CTTTTTAAAA GTGTTTCATG TAGCTCACTA TCAAATAGTA ATTCTGAGCC AGCCAAGTTC 
ATGAAAAACA TTGGACCATC TTCATTTGTA GATCATGACT TCCTTAAACG CCGATTGCCA 
AAGTTGAGCA AGTCCACAAC TCCATCTCTT GCTCTCTTAA CTGATAGTGA AAAACCATCT 
CACAAGGCTT TTGCTACTCA CAAATTATCC TCCAGTATGT GTGTCTCTAG TGACCTTTTG 
TCTGATATTT ATAAGCCCAA AAGAGGAAGA CCTAAAGCTA AAGAGATGCC TCAACTGGAA 
GGTCCACCTA AAAGGACTTT AAAAATCCCT GCCTCTAAAG TTTTTTCTTT GCAGTCTAAG 
GAAGAACAAG AACCCCCAAT TTTACAGCCA GAAATTGAAA TTCCTTCCTT CAAACAAAGT 
CTTTCTGTAT CTCCTTTTCC AAAAAAGCGA GGCAGACCTA AGAGGCAGTT GAGGTCACCG 
GTCAAGATGA AGCCACCTGT ACTATCAGTG GCTCCATTTG TTGCCACGGA GAGTCCAAGC 
AAGCTGGAGT CTGAAAGTGA TAACCATCGA AGCAGCAGTG ATTTCTTTGA GAGTGAGGAT 
CAGCTTCAGG ACCCAGATGA TCTGGACGAC AGTCATAGGC CGGCTGTCTG CAGCATGAGT 
GACCTTGAGA TGGAACCAGA TAAAAAAATT ACAAAGAGAA ACAATGGACA ATTAATGAAA 
ACAATTATCC GCAAAATAAA TAAAATGAAG ACTTTAAAAA GGAAGAAACT GTTGAATCAG 
ATTCTTTCAA GCTCTGTAGA ATCAAGTAAT AAAGGGAAAG TGCAATCCAA ACTCCATAAT 
ACAGTGTCAA GTCTTGCTGC CACGTTTGGC TCTAAATTGG GCCAACAGAT AAATGTCAGC 
AAGAAAGGAA CCATTTACAT AGGAAAGAGA AGGGGTCGAA AACCAAAAAC TGTCTTAAAT 
GGCATTCTTT CTGGTAGCCC TACCAGCCTT GCTGTCCTTG AGCAAACTGC TCAACAGGCT 
GCCGGCTCAG CATTGGGACA GATACTTCCC CCTTTACTGC CTTCATCTGC TAGTAGCTCA 
GAGATTCTCC CATCGCCTGT TTGCTCTCAG TCTTCTGGGA CTAGTGGTGG TCAGAGCCCT 
GTAAGTAGTG ATGCAGGCTT TGTTGAACCC AGCTCAGTGC CATATTTGCA TTTACACTCC 
AGACAGGGCA GTATGATTCA GACTCTTGCG ATGAAAAAGG CCTCAAAGGG GAGGAGGCGA 
CTTTCTCCTC CTACTTTGTT GCCAAATTCT CCTTCACACT TGAGTGAACT CACCTCTCTG 
AAAGAAGCTA CTCCTTCTCC CATTAGTGAG TCTCATAGTG ATGAGACCAT TCCCAGTGAT 
AGTGGAATTG GAACAGATAA TAACAGCACA TCGGACAGAG CAGAGAAATT TTGTGGACAG 
AAAAAGAGGA GGCACTCTTT TGAACATGTT TCTCTGATTC CCCCTGAAAC CTCTACAGTC 
CTGAGCAGTC TTAAAGAAAA ACATAAACAT AAATGTAAGC GCCGGAATCA CGATTACCTC 
AGCTATGACA AGATGAAAAG GCAGAAACGA AAACGGAAAA AGAAATACCC CCAGCTCCGT 
AGCAGACAGG ATCCAGACTT TATTGCGGAT CTGGAGGAAT TGATAAGTCG GCTGAGTGAA 
ATTCGAATCA CCCATCGAAG CCATCATTTT ATCCCCCGAG ACCTGCTGCC AACTATTTTT 
CGAATCAACT TTAATAGTTT CTATACACAT CCTTCTTTCC CCTTAGACCC CTTGCACTAC 
ATTCGAAAAC CTGACTTAAA AAAGAAGAGA GGGAGACCTC CTAAGATGAG GGAGGCAATG 
GCTGAGATGC CCTTCATGCA CAGCCTTAGT TTTCCTCTTT CTAGTACTGG ATTCTATCCC 
TCCTATGGCA TGCCTTACTC TCCCTCACCC CTTACAGCCA CTCCCCTAGG ATTAGGTTAC 
TATGGAAGGT ACCCTCCCAC TCTCTATCCT CCTCCTCCTT CACCTTCTTT CACCCCCCCA 
CTTCCTCCTC CTTCCTATAT GCATGCTGGC CATTTACTTC TCAATCCAAC CAAATACCAT 
AAGAAAAAGC ATAAGCTACT TCGCCAGGAG GCCTTTCTAA CAACCAGCAG GACTCCCCTC 
CTTTCCATGA GTACCTACCC TAGTGTTCCT CCTGAGATGG CCTATGGTTG GATGGTTGAG 
CACAAACATA GGCATCGTCA CAAGCACAGA GAACACCGTT CTTCTGAGCA ACCCCAGGTT 
TCCATGGATG CTGGCTCTTC CAGATCTGTT TTGGAGTCTT TGAAGCGCTA TCGATTTGGA 
AAGGAAACTG TTGGAGAGCG ATATAAACAT AAGGAAAAGC ACCGATGTCA TATGTCCTGC 
CCTCATCTCT CTCCTTCGAA AAGCTTAATA AATAGAGAAG AACAATGGGT CCACCGAGAG 
CCTTCAGAAT CTAGTCCATT GGCTTTGGGA TTGCAAACAC CTTTACAGAT TGACTGTTCC 
GAAAGTTCTC CAAGTTTATC TCTTGGAGGA TTCACTCCCA ACTCTGATCC GGCCAGCAGT 
GATGAACATA CAAACCTTTT CACAAGTGCA ATAGTGCAAG GCAGCTGCAG AGTTTCAAAC 
CCTAACTCCA GTGGCCGGAA GAAATTAACT GATAGCCCTG GACTGTTTTC TGCACAGGAC 
ACTTCACTAA TTCGGCCTCA CAGAAAGGAG CCACTGCCTT CCAGCGAAAG GGCAGTACAG 
ACCATGACAG GTTCCCAGCC AACCTCAGAT AAGTCTTCCC AGCGACCATC AGAGAGCACA 
AACTGTAGCC CTACCCGGAA GAGGTCTTCA TCTGAGAGTA CTTCTTCAAC AGTAAACGGA 
CTTCCTTCTC GAAGTCCAAG ATTGCTTGCC CCTGGGGATG ACTCTGTGGA TAGTCTGCTG 
CAGCGGATGG TACAACATGA GGACCAAGAG CCCCTGGAGA AGAATCTAGA TGCCGTGATT 
GCATCTGCCT CGGGGCCACC TTCTTCCAGT CCAGGCCACA GCCACAGCAA GGAACGAGCC 
CTGGGAAAAC CAGATAGCCT TCTAGTGCCC GCAGCCCCCA GTGACTCTTG TAGCAGCAGC 
ATCTCACTCC TGTCTGAGAA GTTGCCGAGC AGCTGCTCAC CCCACCATAT CAAGAGGAGT 
GTCGTGGAAG CCATGCAGCG CCAAGCTCGG AAAATGTGCA ATTATGACAA AATCTTGGCC 
ACAAAGAAAA ACCTGGACCA TGTCAATAAA ATATTAAAAG CCAAAAAGCT TCAAAGACAG 
GCCAGGACAG GGAATAACTT CGTGAAACGC AGACCAGGGC GACCTCGGAA ATGCCCCCTC 
CAGGCAGTGG TGTCAATGCA AGCATTCCAG GCTGCCCGAT TCGTCAGCCC CGATGCTAAT 
GAAGGTGAAG AAAGAGCAGC CCTGCCCCTC CGTCCAGACA CAGTTACGGA TGTCATCGAG 
GCTGTTGTGC AGAGCGTAAA CCTGAGTCCA GAACATAAGA AGGGGCTGAA GAGGAAAAGT 
TGGCTGTTGG AAGAACAGAC CAAGAAAAAG CAGAAGCCAT TCCCAGAGGA AGAACAAGAG 
CATCCCAAGA GCTTTACTGA AGCAGCAGTT GAGATTCCCA GTCCTCCTGA AACCCCAGCC 
AAGCCTTCCG AACCTGAAAG TACCTTGCAG CCTGTGCTTT CTCTCATCCC AAGGGAAAAG 
AAGGCCCCAC GTCCCCCAAA GAAGAAGTAT CAGAAAGCAG GGCTGTATTC TGACGTTTAC 
AAAACTACAG ACCCAAAGAG CCGATTGATC CAATTAAAGA AAGAAAAGTT GGAATACACT 
CCAGGAGAGC ATGAGTATGG ATTATTCCCA GCCCCTATTC ATGTTGGAAA GTATCTAAGG 
CAAAAGAGAA TTGACTTCCA GCTTCCCTAT GACATCCTTT GGCAGTGGAA ACATAATCAG 
CTATACAAAA AGCCAGATGT CCCACTATAT AAAAAAATCC GTTCAAACGT CTACGTGGAT 
GTCAAACCCC TTTCTGGTTA CGAGGCCACC ACCTGTAACT GTAAGAAGCC AGAGGATGAC 
ACCGGGAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCT 
AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGAGGCA TGAGTGGGTG 
CAGTGTCTAG AACGATTTCG AGCTGAGGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC 
CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGGGAGG TTGTCAGCGA ACAGGAGTTC 
AGGAACAGAA TGATTGAGCA GTATCATAAT CACAGTGACC ACTATTGCCT GAACCTGGAT 
AGTGGGATGG TGATTGACAG TTATCGGATG GGTAATGAGG CCCGATTCAT CAACCACAGC 
TGTGACCCAA ACTGTGAAAT GCAGAAATGG TCTGTCAATG GAGTATACCG GATTGGACTG 
TATGCTCTTA AAGATATGCC AGCTGGTACC GAGCTCACTT ATGATTACAA TTTCCATTCC 
TTTAATGTTG AGAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT 
GGGGGCAAGA GTCAGCGCGT GAACGGACTC GCCAGCAGCA AAAGCAGCCA GCCTGTGGCT 
ACCCATAAAA AGTCTGGACG GTCAAAAGAG AAGAGAAAGT CAAAGCACAA GCTGAAGAAA 
AGGAGAGGCC ACCTCTCCGA AGAACCCAGT GAAAACATCA ACACTCCAAC AAGATTGACC 
CCCCAATTAC AAATGAAGCC AATGTCTAAT CGAGAGAGGA ACTTCGTGTT AAAGCATCAT 
GTATTCTTGG TCCGAAACTG GGAGAAGATC CGACAGAAAC AAGAGGAAGT AAAGCATACC 
AGCGATAATC TCCACTCTGC ATCATTATAC ACCCGCTGGA ATGGCATCTG CCGAGATGAT 
GGGAACATCA AGTCTGACGT CTTCATGACC CAGTTCTCCG CCCTGCAGAC AGCACGATCA 
GTTCGAACAA GACGCCTGGC AGCAGCAGAG GAAAACATCG AGGTGGCACG GGCAGCCCGC 
CTCGCCCAAA TCTTCAAAGA GATCTGTGAT GGCATCATCT CCTACAAAGA TTCTTCCCGA 
CAAGCTTTGG CAGCACCCCT CTTGAACCTC CCCCCAAAGA AAAAGAATGC TGATTACTAT 
GAGAAGATCT CAGAGCCCCT GGACTTGGCC ACCATAGAGA GGCAGATCCT CACCGGCCAT 
TACAAGACCG TGGAGGCCTT TGACGCTGAC ATGCTCAAGG TGTTTCGGAA TGCTGAGAAG 
TACTACGGGC GTAAATCCCC AATTGGGAGA GATGTTTGCC GTCTACGGAA GACCTACTAC 
AACGCCAGGC ATGAGGCATC AGCCCAGATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG 
GCCGACAGCA GCGAAACATC TGTCTCTGAG AAGGAGAACG TGCATGAGAA GGATGACGAT 
GTGATCCGCT GTGTCTGTGG CCTCCACAAG GATGAGGGCC TCATGATCCA GTGTGACAAG 
TGCATGGTAT GGCAGCACTG TGACTGTATG GGGGTGAACT CAGACGTGGA GCATTACCTG 
TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC 
TATGCCCAGC CCGGCTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GTTGCTTCGT 
CAAGGTGACT GTGTGTACCT GATGCGAGAT AGCCGGCGCA CCCCTGATGG CCACCCAGTC 
CGCCAGTCCT ACCGACTGTT ATCACACATT AACCGAGATA AACTTGACAT CTTCCGCATT 
GAGAAGCTCT GGAAGAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA CTACTTCCGC 
CCCCACGAGA CCCACCACTC TCCGTCACGC CGCTTCTATC ACAATGAGCT ATTTAGGGTA 
CCACTCTATG AGATCATTCC CTTGGAGGCT GTGGTGGGGA CCTGCTGTGT ACTAGACCTT 
TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTATA CATCTGTGAC 
TATCGGCTTG ACAAGTCCGC ACACCTGTTT TACAAGATTC ACCGGAATCG CTATCCTGTC 
TGCACCAAAC CCTACGCCTT TGACCACTTC CCCAAGAAGC TCACTCCTAA AAGAGATTTC 
TCACCTCATT ATGTCCCAGA CAACTACAAG AGGAACGGAG GGCGGTCATC CTGGAAGTCT 
GAGCGCCCAA AGCCACCTCT AAAAGACCTG GGCCAGGAGG ATGACGCTCT GCCCTTGATT 
GAAGAGGTTC TGGCCAGCCA GGAGCAAGCC ACCAATGAGC TGCCCAGCCT GGAGGAGCCA 
GAACGGGAAG TGGCCCCTGT GGAAGTCAGC GAGGGTGAAA AGAAAGCAGA GGAAGGTGGT 
CCAGAATCTC AGGCTGCCTG TACCCCTGAG GAGCGACGGC ACAGCCAACG GGAACGGCTC 
AACCAGATCC TACTTGGCCT CCTGGAAAAA ATCCCTGGGA AAAATGCCAT CGATGTGACC 
TACTTGCTGG AGGAAGGGTC AGGCAGGAAG CTTCGAAGGC GCACTTTGTT CATCCCAGAG 
AACAGCTTTC GGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_537251.2
CDS	544..9441
Translation	MDPRNTAMLGLGSDSEGFSRKSPSALGTGTVVSKREGELESNTKEEEDLRKRNWDRNSEAGKEDGLTDAQQQFSVKETNFSEGNLKLKIGLQAKRTKKPPKNLENYVCRPAIKTTIKHPRKALKGGKMTEEKDEHCPSRRDPSKLYKKTGDVAAVECQSEESIHLHSQGENSPLSKKLSPGHSEMTDYMNAASSTLVGSRDPDLKDRALLNGGTSVTEKLAQLIATCPPSKSSKTKPKKLGSGTSAGLVSKDLIRKAGVGSVAGIIHKDLIKKPAISTAVGLVTKDPGKKPVFNATVGLVNKDSVKKLGAGTTAVFINKDLGRKPGTVTTVGLLSKDSGKKLGIGIVPGLVNKESGKKLGLGTVVGLVNKDLGKKLSSTVGLVAKDCAKKIVANSTMGLVTKDMGKKLVSCSMAGLVNKDAINLKAEALLPTQEPLKASCSTNISSHENQELSESLKDSTTSKTFEKNVIRQSKESILEKFSVRKEIINLEKEMFNEGTCIQQDSFSSNDRGPYETSKHEKQPPVYCTSPDFQMGAASDASMAKSPFSAVGESNLPSPSPTVSVNPLTRSPPETSSQLAPNPLLLSPTTELMEDISESVGKNQFTSESTHLNIGHRSVGHSMNIECKGIDKELTDSKTAHIDIPRISSSLGKKPSLTSESSIHTITPSVVNFTSLFSNKPFLKLGAVSASDKHCQVAESLSSSLQSKPLKKRKGRKARWTKVVARSTCRSPKGLDLERSELFKSVSCSSLSNSNSEPAKFMKNIGPSSFVDHDFLKRRLPKLSKSTTPSLALLTDSEKPSHKAFATHKLSSSMCVSSDLLSDIYKPKRGRPKAKEMPQLEGPPKRTLKIPASKVFSLQSKEEQEPPILQPEIEIPSFKQSLSVSPFPKKRGRPKRQLRSPVKMKPPVLSVAPFVATESPSKLESESDNHRSSSDFFESEDQLQDPDDLDDSHRPAVCSMSDLEMEPDKKITKRNNGQLMKTIIRKINKMKTLKRKKLLNQILSSSVESSNKGKVQSKLHNTVSSLAATFGSKLGQQINVSKKGTIYIGKRRGRKPKTVLNGILSGSPTSLAVLEQTAQQAAGSALGQILPPLLPSSASSSEILPSPVCSQSSGTSGGQSPVSSDAGFVEPSSVPYLHLHSRQGSMIQTLAMKKASKGRRRLSPPTLLPNSPSHLSELTSLKEATPSPISESHSDETIPSDSGIGTDNNSTSDRAEKFCGQKKRRHSFEHVSLIPPETSTVLSSLKEKHKHKCKRRNHDYLSYDKMKRQKRKRKKKYPQLRSRQDPDFIADLEELISRLSEIRITHRSHHFIPRDLLPTIFRINFNSFYTHPSFPLDPLHYIRKPDLKKKRGRPPKMREAMAEMPFMHSLSFPLSSTGFYPSYGMPYSPSPLTATPLGLGYYGRYPPTLYPPPPSPSFTPPLPPPSYMHAGHLLLNPTKYHKKKHKLLRQEAFLTTSRTPLLSMSTYPSVPPEMAYGWMVEHKHRHRHKHREHRSSEQPQVSMDAGSSRSVLESLKRYRFGKETVGERYKHKEKHRCHMSCPHLSPSKSLINREEQWVHREPSESSPLALGLQTPLQIDCSESSPSLSLGGFTPNSDPASSDEHTNLFTSAIVQGSCRVSNPNSSGRKKLTDSPGLFSAQDTSLIRPHRKEPLPSSERAVQTMTGSQPTSDKSSQRPSESTNCSPTRKRSSSESTSSTVNGLPSRSPRLLAPGDDSVDSLLQRMVQHEDQEPLEKNLDAVIASASGPPSSSPGHSHSKERALGKPDSLLVPAAPSDSCSSSISLLSEKLPSSCSPHHIKRSVVEAMQRQARKMCNYDKILATKKNLDHVNKILKAKKLQRQARTGNNFVKRRPGRPRKCPLQAVVSMQAFQAARFVSPDANEGEERAALPLRPDTVTDVIEAVVQSVNLSPEHKKGLKRKSWLLEEQTKKKQKPFPEEEQEHPKSFTEAAVEIPSPPETPAKPSEPESTLQPVLSLIPREKKAPRPPKKKYQKAGLYSDVYKTTDPKSRLIQLKKEKLEYTPGEHEYGLFPAPIHVGKYLRQKRIDFQLPYDILWQWKHNQLYKKPDVPLYKKIRSNVYVDVKPLSGYEATTCNCKKPEDDTGKGCVDDCLNRMIFAECSPNTCPCGEQCCNQRIQRHEWVQCLERFRAEEKGWGIRTKEPLKAGQFIIEYLGEVVSEQEFRNRMIEQYHNHSDHYCLNLDSGMVIDSYRMGNEARFINHSCDPNCEMQKWSVNGVYRIGLYALKDMPAGTELTYDYNFHSFNVEKQQLCKCGFEKCRGIIGGKSQRVNGLASSKSSQPVATHKKSGRSKEKRKSKHKLKKRRGHLSEEPSENINTPTRLTPQLQMKPMSNRERNFVLKHHVFLVRNWEKIRQKQEEVKHTSDNLHSASLYTRWNGICRDDGNIKSDVFMTQFSALQTARSVRTRRLAAAEENIEVARAARLAQIFKEICDGIISYKDSSRQALAAPLLNLPPKKKNADYYEKISEPLDLATIERQILTGHYKTVEAFDADMLKVFRNAEKYYGRKSPIGRDVCRLRKTYYNARHEASAQIDEIVGETASEADSSETSVSEKENVHEKDDDVIRCVCGLHKDEGLMIQCDKCMVWQHCDCMGVNSDVEHYLCEQCDPRPVDREVPMIPRPHYAQPGCVYFICLLRDDLLLRQGDCVYLMRDSRRTPDGHPVRQSYRLLSHINRDKLDIFRIEKLWKNEKEERFAFGHHYFRPHETHHSPSRRFYHNELFRVPLYEIIPLEAVVGTCCVLDLYTYCKGRPKGVKEQDVYICDYRLDKSAHLFYKIHRNRYPVCTKPYAFDHFPKKLTPKRDFSPHYVPDNYKRNGGRSSWKSERPKPPLKDLGQEDDALPLIEEVLASQEQATNELPSLEEPEREVAPVEVSEGEKKAEEGGPESQAACTPEERRHSQRERLNQILLGLLEKIPGKNAIDVTYLLEEGSGRKLRRRTLFIPENSFRK

Target ORF information:

RefSeq Version	XM_537251.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ash1 (absent, small, or homeotic)-like (Drosophila) (ASH1L), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_537251.5

ORF Insert Sequence:

ATGGACCCTA GAAATACTGC TATGTTAGGA TTGGGTTCTG ACTCGGAAGG ATTTTCAAGG 
AAGAGTCCCT CTGCCCTTGG TACCGGCACA GTGGTCAGCA AGAGAGAAGG AGAGCTAGAG 
AGCAACACGA AAGAGGAAGA GGACCTTCGC AAGAGGAATT GGGACAGAAA CAGCGAAGCA 
GGGAAGGAGG ATGGTTTGAC TGATGCACAG CAACAGTTTT CAGTGAAAGA AACAAACTTT 
TCAGAGGGAA ACTTAAAATT GAAAATTGGC CTTCAGGCTA AGAGAACTAA AAAACCTCCA 
AAAAACTTGG AGAACTATGT ATGTCGACCT GCCATAAAAA CAACTATCAA GCACCCAAGG 
AAAGCACTTA AAGGTGGGAA GATGACAGAG GAGAAGGATG AGCACTGTCC TTCAAGACGG 
GACCCTTCAA AGTTGTACAA GAAAACAGGT GATGTTGCAG CCGTTGAATG CCAATCTGAA 
GAGAGCATCC ATCTTCATTC ACAGGGAGAA AACAGTCCTT TGTCTAAGAA GCTTTCTCCA 
GGACACTCAG AGATGACAGA TTACATGAAT GCAGCATCAT CTACTCTTGT TGGTAGCCGG 
GATCCTGATT TAAAGGATAG AGCATTACTT AATGGAGGAA CAAGTGTAAC AGAAAAGTTG 
GCACAGCTTA TTGCAACCTG TCCTCCCTCT AAGTCTTCCA AGACTAAACC GAAGAAGTTA 
GGAAGTGGCA CCAGCGCAGG ATTGGTTAGT AAGGATTTGA TCAGGAAAGC AGGTGTTGGC 
TCTGTAGCTG GAATAATACA TAAAGATTTA ATTAAAAAAC CAGCTATCAG CACAGCAGTT 
GGATTGGTAA CTAAAGATCC TGGGAAAAAG CCAGTGTTTA ATGCAACAGT AGGATTGGTC 
AATAAGGACT CTGTGAAAAA ACTAGGAGCT GGCACTACAG CGGTATTCAT TAATAAAGAC 
TTAGGCAGAA AGCCAGGGAC TGTCACTACA GTAGGACTGC TGAGCAAGGA TTCAGGAAAG 
AAGCTAGGAA TTGGTATTGT TCCAGGTTTA GTGAATAAGG AATCTGGAAA GAAGTTAGGA 
CTCGGCACTG TGGTCGGACT GGTTAATAAA GACTTGGGCA AGAAGTTGAG TTCTACTGTC 
GGTCTAGTGG CCAAGGACTG TGCAAAGAAG ATTGTAGCCA ATTCAACAAT GGGATTAGTT 
ACTAAAGACA TGGGAAAGAA ACTAGTGAGT TGTTCTATGG CAGGATTGGT CAATAAAGAT 
GCCATAAACC TTAAAGCCGA AGCACTGCTC CCCACTCAGG AACCACTTAA GGCTTCTTGT 
AGTACAAACA TCAGTAGTCA TGAAAATCAG GAACTTTCTG AATCCCTGAA AGATAGTACC 
ACCAGCAAAA CTTTTGAGAA GAATGTTATA CGGCAGAGTA AAGAAAGCAT ATTGGAAAAG 
TTCTCAGTTC GAAAAGAAAT CATTAATTTG GAGAAAGAAA TGTTTAACGA GGGAACATGC 
ATTCAGCAAG ACAGTTTCTC ATCCAATGAC AGGGGGCCTT ATGAAACCTC AAAGCATGAA 
AAGCAACCTC CCGTGTATTG CACTTCTCCA GACTTTCAAA TGGGAGCTGC TTCTGATGCA 
TCTATGGCAA AATCCCCATT TAGTGCAGTA GGAGAAAGCA ATCTCCCTTC CCCATCACCT 
ACTGTATCTG TTAATCCTTT AACCAGAAGT CCCCCTGAAA CTTCTTCACA GTTGGCTCCT 
AATCCGTTAC TTTTAAGTCC TACCACAGAA CTAATGGAAG ACATTTCTGA ATCCGTTGGG 
AAGAACCAGT TTACTTCTGA AAGTACCCAC TTGAACATTG GTCATAGGTC TGTGGGTCAC 
AGCATGAATA TTGAATGTAA AGGGATTGAT AAAGAGCTAA CTGATTCAAA AACTGCACAT 
ATAGATATTC CAAGAATAAG CTCTTCCCTG GGAAAAAAGC CAAGTTTGAC TTCTGAATCC 
AGTATTCACA CAATTACCCC TTCAGTTGTT AACTTCACTA GTTTATTTAG TAACAAGCCG 
TTTCTAAAAC TTGGTGCAGT ATCTGCTTCT GACAAACACT GCCAGGTTGC TGAAAGCCTA 
AGCTCTAGTT TGCAGTCCAA ACCATTAAAA AAAAGGAAAG GAAGAAAAGC TCGGTGGACT 
AAAGTGGTGG CAAGAAGCAC ATGCCGGTCT CCAAAAGGGC TAGATTTAGA AAGATCAGAG 
CTTTTTAAAA GTGTTTCATG TAGCTCACTA TCAAATAGTA ATTCTGAGCC AGCCAAGTTC 
ATGAAAAACA TTGGACCATC TTCATTTGTA GATCATGACT TCCTTAAACG CCGATTGCCA 
AAGTTGAGCA AGTCCACAAC TCCATCTCTT GCTCTCTTAA CTGATAGTGA AAAACCATCT 
CACAAGGCTT TTGCTACTCA CAAATTATCC TCCAGTATGT GTGTCTCTAG TGACCTTTTG 
TCTGATATTT ATAAGCCCAA AAGAGGAAGA CCTAAAGCTA AAGAGATGCC TCAACTGGAA 
GGTCCACCTA AAAGGACTTT AAAAATCCCT GCCTCTAAAG TTTTTTCTTT GCAGTCTAAG 
GAAGAACAAG AACCCCCAAT TTTACAGCCA GAAATTGAAA TTCCTTCCTT CAAACAAAGT 
CTTTCTGTAT CTCCTTTTCC AAAAAAGCGA GGCAGACCTA AGAGGCAGTT GAGGTCACCG 
GTCAAGATGA AGCCACCTGT ACTATCAGTG GCTCCATTTG TTGCCACGGA GAGTCCAAGC 
AAGCTGGAGT CTGAAAGTGA TAACCATCGA AGCAGCAGTG ATTTCTTTGA GAGTGAGGAT 
CAGCTTCAGG ACCCAGATGA TCTGGACGAC AGTCATAGGC CGGCTGTCTG CAGCATGAGT 
GACCTTGAGA TGGAACCAGA TAAAAAAATT ACAAAGAGAA ACAATGGACA ATTAATGAAA 
ACAATTATCC GCAAAATAAA TAAAATGAAG ACTTTAAAAA GGAAGAAACT GTTGAATCAG 
ATTCTTTCAA GCTCTGTAGA ATCAAGTAAT AAAGGGAAAG TGCAATCCAA ACTCCATAAT 
ACAGTGTCAA GTCTTGCTGC CACGTTTGGC TCTAAATTGG GCCAACAGAT AAATGTCAGC 
AAGAAAGGAA CCATTTACAT AGGAAAGAGA AGGGGTCGAA AACCAAAAAC TGTCTTAAAT 
GGCATTCTTT CTGGTAGCCC TACCAGCCTT GCTGTCCTTG AGCAAACTGC TCAACAGGCT 
GCCGGCTCAG CATTGGGACA GATACTTCCC CCTTTACTGC CTTCATCTGC TAGTAGCTCA 
GAGATTCTCC CATCGCCTGT TTGCTCTCAG TCTTCTGGGA CTAGTGGTGG TCAGAGCCCT 
GTAAGTAGTG ATGCAGGCTT TGTTGAACCC AGCTCAGTGC CATATTTGCA TTTACACTCC 
AGACAGGGCA GTATGATTCA GACTCTTGCG ATGAAAAAGG CCTCAAAGGG GAGGAGGCGA 
CTTTCTCCTC CTACTTTGTT GCCAAATTCT CCTTCACACT TGAGTGAACT CACCTCTCTG 
AAAGAAGCTA CTCCTTCTCC CATTAGTGAG TCTCATAGTG ATGAGACCAT TCCCAGTGAT 
AGTGGAATTG GAACAGATAA TAACAGCACA TCGGACAGAG CAGAGAAATT TTGTGGACAG 
AAAAAGAGGA GGCACTCTTT TGAACATGTT TCTCTGATTC CCCCTGAAAC CTCTACAGTC 
CTGAGCAGTC TTAAAGAAAA ACATAAACAT AAATGTAAGC GCCGGAATCA CGATTACCTC 
AGCTATGACA AGATGAAAAG GCAGAAACGA AAACGGAAAA AGAAATACCC CCAGCTCCGT 
AGCAGACAGG ATCCAGACTT TATTGCGGAT CTGGAGGAAT TGATAAGTCG GCTGAGTGAA 
ATTCGAATCA CCCATCGAAG CCATCATTTT ATCCCCCGAG ACCTGCTGCC AACTATTTTT 
CGAATCAACT TTAATAGTTT CTATACACAT CCTTCTTTCC CCTTAGACCC CTTGCACTAC 
ATTCGAAAAC CTGACTTAAA AAAGAAGAGA GGGAGACCTC CTAAGATGAG GGAGGCAATG 
GCTGAGATGC CCTTCATGCA CAGCCTTAGT TTTCCTCTTT CTAGTACTGG ATTCTATCCC 
TCCTATGGCA TGCCTTACTC TCCCTCACCC CTTACAGCCA CTCCCCTAGG ATTAGGTTAC 
TATGGAAGGT ACCCTCCCAC TCTCTATCCT CCTCCTCCTT CACCTTCTTT CACCCCCCCA 
CTTCCTCCTC CTTCCTATAT GCATGCTGGC CATTTACTTC TCAATCCAAC CAAATACCAT 
AAGAAAAAGC ATAAGCTACT TCGCCAGGAG GCCTTTCTAA CAACCAGCAG GACTCCCCTC 
CTTTCCATGA GTACCTACCC TAGTGTTCCT CCTGAGATGG CCTATGGTTG GATGGTTGAG 
CACAAACATA GGCATCGTCA CAAGCACAGA GAACACCGTT CTTCTGAGCA ACCCCAGGTT 
TCCATGGATG CTGGCTCTTC CAGATCTGTT TTGGAGTCTT TGAAGCGCTA TCGATTTGGA 
AAGGAAACTG TTGGAGAGCG ATATAAACAT AAGGAAAAGC ACCGATGTCA TATGTCCTGC 
CCTCATCTCT CTCCTTCGAA AAGCTTAATA AATAGAGAAG AACAATGGGT CCACCGAGAG 
CCTTCAGAAT CTAGTCCATT GGCTTTGGGA TTGCAAACAC CTTTACAGAT TGACTGTTCC 
GAAAGTTCTC CAAGTTTATC TCTTGGAGGA TTCACTCCCA ACTCTGATCC GGCCAGCAGT 
GATGAACATA CAAACCTTTT CACAAGTGCA ATAGTGCAAG GCAGCTGCAG AGTTTCAAAC 
CCTAACTCCA GTGGCCGGAA GAAATTAACT GATAGCCCTG GACTGTTTTC TGCACAGGAC 
ACTTCACTAA TTCGGCCTCA CAGAAAGGAG CCACTGCCTT CCAGCGAAAG GGCAGTACAG 
ACCATGACAG GTTCCCAGCC AACCTCAGAT AAGTCTTCCC AGCGACCATC AGAGAGCACA 
AACTGTAGCC CTACCCGGAA GAGGTCTTCA TCTGAGAGTA CTTCTTCAAC AGTAAACGGA 
CTTCCTTCTC GAAGTCCAAG ATTGCTTGCC CCTGGGGATG ACTCTGTGGA TAGTCTGCTG 
CAGCGGATGG TACAACATGA GGACCAAGAG CCCCTGGAGA AGAATCTAGA TGCCGTGATT 
GCATCTGCCT CGGGGCCACC TTCTTCCAGT CCAGGCCACA GCCACAGCAA GGAACGAGCC 
CTGGGAAAAC CAGATAGCCT TCTAGTGCCC GCAGCCCCCA GTGACTCTTG TAGCAGCAGC 
ATCTCACTCC TGTCTGAGAA GTTGCCGAGC AGCTGCTCAC CCCACCATAT CAAGAGGAGT 
GTCGTGGAAG CCATGCAGCG CCAAGCTCGG AAAATGTGCA ATTATGACAA AATCTTGGCC 
ACAAAGAAAA ACCTGGACCA TGTCAATAAA ATATTAAAAG CCAAAAAGCT TCAAAGACAG 
GCCAGGACAG GGAATAACTT CGTGAAACGC AGACCAGGGC GACCTCGGAA ATGCCCCCTC 
CAGGCAGTGG TGTCAATGCA AGCATTCCAG GCTGCCCGAT TCGTCAGCCC CGATGCTAAT 
GAAGGTGAAG AAAGAGCAGC CCTGCCCCTC CGTCCAGACA CAGTTACGGA TGTCATCGAG 
GCTGTTGTGC AGAGCGTAAA CCTGAGTCCA GAACATAAGA AGGGGCTGAA GAGGAAAAGT 
TGGCTGTTGG AAGAACAGAC CAAGAAAAAG CAGAAGCCAT TCCCAGAGGA AGAACAAGAG 
CATCCCAAGA GCTTTACTGA AGCAGCAGTT GAGATTCCCA GTCCTCCTGA AACCCCAGCC 
AAGCCTTCCG AACCTGAAAG TACCTTGCAG CCTGTGCTTT CTCTCATCCC AAGGGAAAAG 
AAGGCCCCAC GTCCCCCAAA GAAGAAGTAT CAGAAAGCAG GGCTGTATTC TGACGTTTAC 
AAAACTACAG ACCCAAAGAG CCGATTGATC CAATTAAAGA AAGAAAAGTT GGAATACACT 
CCAGGAGAGC ATGAGTATGG ATTATTCCCA GCCCCTATTC ATGTTGGAAA GTATCTAAGG 
CAAAAGAGAA TTGACTTCCA GCTTCCCTAT GACATCCTTT GGCAGTGGAA ACATAATCAG 
CTATACAAAA AGCCAGATGT CCCACTATAT AAAAAAATCC GTTCAAACGT CTACGTGGAT 
GTCAAACCCC TTTCTGGTTA CGAGGCCACC ACCTGTAACT GTAAGAAGCC AGAGGATGAC 
ACCGGGAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCT 
AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGAGGCA TGAGTGGGTG 
CAGTGTCTAG AACGATTTCG AGCTGAGGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC 
CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGGGAGG TTGTCAGCGA ACAGGAGTTC 
AGGAACAGAA TGATTGAGCA GTATCATAAT CACAGTGACC ACTATTGCCT GAACCTGGAT 
AGTGGGATGG TGATTGACAG TTATCGGATG GGTAATGAGG CCCGATTCAT CAACCACAGC 
TGTGACCCAA ACTGTGAAAT GCAGAAATGG TCTGTCAATG GAGTATACCG GATTGGACTG 
TATGCTCTTA AAGATATGCC AGCTGGTACC GAGCTCACTT ATGATTACAA TTTCCATTCC 
TTTAATGTTG AGAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT 
GGGGGCAAGA GTCAGCGCGT GAACGGACTC GCCAGCAGCA AAAGCAGCCA GCCTGTGGCT 
ACCCATAAAA AGTCTGGACG GTCAAAAGAG AAGAGAAAGT CAAAGCACAA GCTGAAGAAA 
AGGAGAGGCC ACCTCTCCGA AGAACCCAGT GAAAACATCA ACACTCCAAC AAGATTGACC 
CCCCAATTAC AAATGAAGCC AATGTCTAAT CGAGAGAGGA ACTTCGTGTT AAAGCATCAT 
GTATTCTTGG TCCGAAACTG GGAGAAGATC CGACAGAAAC AAGAGGAAGT AAAGCATACC 
AGCGATAATC TCCACTCTGC ATCATTATAC ACCCGCTGGA ATGGCATCTG CCGAGATGAT 
GGGAACATCA AGTCTGACGT CTTCATGACC CAGTTCTCCG CCCTGCAGAC AGCACGATCA 
GTTCGAACAA GACGCCTGGC AGCAGCAGAG GAAAACATCG AGGTGGCACG GGCAGCCCGC 
CTCGCCCAAA TCTTCAAAGA GATCTGTGAT GGCATCATCT CCTACAAAGA TTCTTCCCGA 
CAAGCTTTGG CAGCACCCCT CTTGAACCTC CCCCCAAAGA AAAAGAATGC TGATTACTAT 
GAGAAGATCT CAGAGCCCCT GGACTTGGCC ACCATAGAGA GGCAGATCCT CACCGGCCAT 
TACAAGACCG TGGAGGCCTT TGACGCTGAC ATGCTCAAGG TGTTTCGGAA TGCTGAGAAG 
TACTACGGGC GTAAATCCCC AATTGGGAGA GATGTTTGCC GTCTACGGAA GACCTACTAC 
AACGCCAGGC ATGAGGCATC AGCCCAGATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG 
GCCGACAGCA GCGAAACATC TGTCTCTGAG AAGGAGAACG TGCATGAGAA GGATGACGAT 
GTGATCCGCT GTGTCTGTGG CCTCCACAAG GATGAGGGCC TCATGATCCA GTGTGACAAG 
TGCATGGTAT GGCAGCACTG TGACTGTATG GGGGTGAACT CAGACGTGGA GCATTACCTG 
TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC 
TATGCCCAGC CCGGCTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GTTGCTTCGT 
CAAGGTGACT GTGTGTACCT GATGCGAGAT AGCCGGCGCA CCCCTGATGG CCACCCAGTC 
CGCCAGTCCT ACCGACTGTT ATCACACATT AACCGAGATA AACTTGACAT CTTCCGCATT 
GAGAAGCTCT GGAAGAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA CTACTTCCGC 
CCCCACGAGA CCCACCACTC TCCGTCACGC CGCTTCTATC ACAATGAGCT ATTTAGGGTA 
CCACTCTATG AGATCATTCC CTTGGAGGCT GTGGTGGGGA CCTGCTGTGT ACTAGACCTT 
TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTATA CATCTGTGAC 
TATCGGCTTG ACAAGTCCGC ACACCTGTTT TACAAGATTC ACCGGAATCG CTATCCTGTC 
TGCACCAAAC CCTACGCCTT TGACCACTTC CCCAAGAAGC TCACTCCTAA AAGAGATTTC 
TCACCTCATT ATGTCCCAGA CAACTACAAG AGGAACGGAG GGCGGTCATC CTGGAAGTCT 
GAGCGCCCAA AGCCACCTCT AAAAGACCTG GGCCAGGAGG ATGACGCTCT GCCCTTGATT 
GAAGAGGTTC TGGCCAGCCA GGAGCAAGCC ACCAATGAGC TGCCCAGCCT GGAGGAGCCA 
GAACGGGAAG TGGCCCCTGT GGAAGTCAGC GAGGGTGAAA AGAAAGCAGA GGAAGGTGGT 
CCAGAATCTC AGGCTGCCTG TACCCCTGAG GAGCGACGGC ACAGCCAACG GGAACGGCTC 
AACCAGATCC TACTTGGCCT CCTGGAAAAA ATCCCTGGGA AAAATGCCAT CGATGTGACC 
TACTTGCTGG AGGAAGGGTC AGGCAGGAAG CTTCGAAGGC GCACTTTGTT CATCCCAGAG 
AACAGCTTTC GGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASH1L ( XM_014115337.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013970812.1 Canis lupus familiaris ash1 (absent, small, or homeotic)-like (Drosophila) (ASH1L), transcript variant X3, mRNA.

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CloneID	OCe09533
Clone ID Related Accession (Same CDS sequence)	XM_014115336.2 , XM_537251.6 , XM_014115338.2 , XM_537251.5 , XM_014115337.1 , XM_014115338.1 , XM_014115336.1 , XM_014115337.2
Accession Version	XM_014115337.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8898bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	histone-lysine N-methyltransferase ASH1L
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCTA GAAATACTGC TATGTTAGGA TTGGGTTCTG ACTCGGAAGG ATTTTCAAGG 
AAGAGTCCCT CTGCCCTTGG TACCGGCACA GTGGTCAGCA AGAGAGAAGG AGAGCTAGAG 
AGCAACACGA AAGAGGAAGA GGACCTTCGC AAGAGGAATT GGGACAGAAA CAGCGAAGCA 
GGGAAGGAGG ATGGTTTGAC TGATGCACAG CAACAGTTTT CAGTGAAAGA AACAAACTTT 
TCAGAGGGAA ACTTAAAATT GAAAATTGGC CTTCAGGCTA AGAGAACTAA AAAACCTCCA 
AAAAACTTGG AGAACTATGT ATGTCGACCT GCCATAAAAA CAACTATCAA GCACCCAAGG 
AAAGCACTTA AAGGTGGGAA GATGACAGAG GAGAAGGATG AGCACTGTCC TTCAAGACGG 
GACCCTTCAA AGTTGTACAA GAAAACAGGT GATGTTGCAG CCGTTGAATG CCAATCTGAA 
GAGAGCATCC ATCTTCATTC ACAGGGAGAA AACAGTCCTT TGTCTAAGAA GCTTTCTCCA 
GGACACTCAG AGATGACAGA TTACATGAAT GCAGCATCAT CTACTCTTGT TGGTAGCCGG 
GATCCTGATT TAAAGGATAG AGCATTACTT AATGGAGGAA CAAGTGTAAC AGAAAAGTTG 
GCACAGCTTA TTGCAACCTG TCCTCCCTCT AAGTCTTCCA AGACTAAACC GAAGAAGTTA 
GGAAGTGGCA CCAGCGCAGG ATTGGTTAGT AAGGATTTGA TCAGGAAAGC AGGTGTTGGC 
TCTGTAGCTG GAATAATACA TAAAGATTTA ATTAAAAAAC CAGCTATCAG CACAGCAGTT 
GGATTGGTAA CTAAAGATCC TGGGAAAAAG CCAGTGTTTA ATGCAACAGT AGGATTGGTC 
AATAAGGACT CTGTGAAAAA ACTAGGAGCT GGCACTACAG CGGTATTCAT TAATAAAGAC 
TTAGGCAGAA AGCCAGGGAC TGTCACTACA GTAGGACTGC TGAGCAAGGA TTCAGGAAAG 
AAGCTAGGAA TTGGTATTGT TCCAGGTTTA GTGAATAAGG AATCTGGAAA GAAGTTAGGA 
CTCGGCACTG TGGTCGGACT GGTTAATAAA GACTTGGGCA AGAAGTTGAG TTCTACTGTC 
GGTCTAGTGG CCAAGGACTG TGCAAAGAAG ATTGTAGCCA ATTCAACAAT GGGATTAGTT 
ACTAAAGACA TGGGAAAGAA ACTAGTGAGT TGTTCTATGG CAGGATTGGT CAATAAAGAT 
GCCATAAACC TTAAAGCCGA AGCACTGCTC CCCACTCAGG AACCACTTAA GGCTTCTTGT 
AGTACAAACA TCAGTAGTCA TGAAAATCAG GAACTTTCTG AATCCCTGAA AGATAGTACC 
ACCAGCAAAA CTTTTGAGAA GAATGTTATA CGGCAGAGTA AAGAAAGCAT ATTGGAAAAG 
TTCTCAGTTC GAAAAGAAAT CATTAATTTG GAGAAAGAAA TGTTTAACGA GGGAACATGC 
ATTCAGCAAG ACAGTTTCTC ATCCAATGAC AGGGGGCCTT ATGAAACCTC AAAGCATGAA 
AAGCAACCTC CCGTGTATTG CACTTCTCCA GACTTTCAAA TGGGAGCTGC TTCTGATGCA 
TCTATGGCAA AATCCCCATT TAGTGCAGTA GGAGAAAGCA ATCTCCCTTC CCCATCACCT 
ACTGTATCTG TTAATCCTTT AACCAGAAGT CCCCCTGAAA CTTCTTCACA GTTGGCTCCT 
AATCCGTTAC TTTTAAGTCC TACCACAGAA CTAATGGAAG ACATTTCTGA ATCCGTTGGG 
AAGAACCAGT TTACTTCTGA AAGTACCCAC TTGAACATTG GTCATAGGTC TGTGGGTCAC 
AGCATGAATA TTGAATGTAA AGGGATTGAT AAAGAGCTAA CTGATTCAAA AACTGCACAT 
ATAGATATTC CAAGAATAAG CTCTTCCCTG GGAAAAAAGC CAAGTTTGAC TTCTGAATCC 
AGTATTCACA CAATTACCCC TTCAGTTGTT AACTTCACTA GTTTATTTAG TAACAAGCCG 
TTTCTAAAAC TTGGTGCAGT ATCTGCTTCT GACAAACACT GCCAGGTTGC TGAAAGCCTA 
AGCTCTAGTT TGCAGTCCAA ACCATTAAAA AAAAGGAAAG GAAGAAAAGC TCGGTGGACT 
AAAGTGGTGG CAAGAAGCAC ATGCCGGTCT CCAAAAGGGC TAGATTTAGA AAGATCAGAG 
CTTTTTAAAA GTGTTTCATG TAGCTCACTA TCAAATAGTA ATTCTGAGCC AGCCAAGTTC 
ATGAAAAACA TTGGACCATC TTCATTTGTA GATCATGACT TCCTTAAACG CCGATTGCCA 
AAGTTGAGCA AGTCCACAAC TCCATCTCTT GCTCTCTTAA CTGATAGTGA AAAACCATCT 
CACAAGGCTT TTGCTACTCA CAAATTATCC TCCAGTATGT GTGTCTCTAG TGACCTTTTG 
TCTGATATTT ATAAGCCCAA AAGAGGAAGA CCTAAAGCTA AAGAGATGCC TCAACTGGAA 
GGTCCACCTA AAAGGACTTT AAAAATCCCT GCCTCTAAAG TTTTTTCTTT GCAGTCTAAG 
GAAGAACAAG AACCCCCAAT TTTACAGCCA GAAATTGAAA TTCCTTCCTT CAAACAAAGT 
CTTTCTGTAT CTCCTTTTCC AAAAAAGCGA GGCAGACCTA AGAGGCAGTT GAGGTCACCG 
GTCAAGATGA AGCCACCTGT ACTATCAGTG GCTCCATTTG TTGCCACGGA GAGTCCAAGC 
AAGCTGGAGT CTGAAAGTGA TAACCATCGA AGCAGCAGTG ATTTCTTTGA GAGTGAGGAT 
CAGCTTCAGG ACCCAGATGA TCTGGACGAC AGTCATAGGC CGGCTGTCTG CAGCATGAGT 
GACCTTGAGA TGGAACCAGA TAAAAAAATT ACAAAGAGAA ACAATGGACA ATTAATGAAA 
ACAATTATCC GCAAAATAAA TAAAATGAAG ACTTTAAAAA GGAAGAAACT GTTGAATCAG 
ATTCTTTCAA GCTCTGTAGA ATCAAGTAAT AAAGGGAAAG TGCAATCCAA ACTCCATAAT 
ACAGTGTCAA GTCTTGCTGC CACGTTTGGC TCTAAATTGG GCCAACAGAT AAATGTCAGC 
AAGAAAGGAA CCATTTACAT AGGAAAGAGA AGGGGTCGAA AACCAAAAAC TGTCTTAAAT 
GGCATTCTTT CTGGTAGCCC TACCAGCCTT GCTGTCCTTG AGCAAACTGC TCAACAGGCT 
GCCGGCTCAG CATTGGGACA GATACTTCCC CCTTTACTGC CTTCATCTGC TAGTAGCTCA 
GAGATTCTCC CATCGCCTGT TTGCTCTCAG TCTTCTGGGA CTAGTGGTGG TCAGAGCCCT 
GTAAGTAGTG ATGCAGGCTT TGTTGAACCC AGCTCAGTGC CATATTTGCA TTTACACTCC 
AGACAGGGCA GTATGATTCA GACTCTTGCG ATGAAAAAGG CCTCAAAGGG GAGGAGGCGA 
CTTTCTCCTC CTACTTTGTT GCCAAATTCT CCTTCACACT TGAGTGAACT CACCTCTCTG 
AAAGAAGCTA CTCCTTCTCC CATTAGTGAG TCTCATAGTG ATGAGACCAT TCCCAGTGAT 
AGTGGAATTG GAACAGATAA TAACAGCACA TCGGACAGAG CAGAGAAATT TTGTGGACAG 
AAAAAGAGGA GGCACTCTTT TGAACATGTT TCTCTGATTC CCCCTGAAAC CTCTACAGTC 
CTGAGCAGTC TTAAAGAAAA ACATAAACAT AAATGTAAGC GCCGGAATCA CGATTACCTC 
AGCTATGACA AGATGAAAAG GCAGAAACGA AAACGGAAAA AGAAATACCC CCAGCTCCGT 
AGCAGACAGG ATCCAGACTT TATTGCGGAT CTGGAGGAAT TGATAAGTCG GCTGAGTGAA 
ATTCGAATCA CCCATCGAAG CCATCATTTT ATCCCCCGAG ACCTGCTGCC AACTATTTTT 
CGAATCAACT TTAATAGTTT CTATACACAT CCTTCTTTCC CCTTAGACCC CTTGCACTAC 
ATTCGAAAAC CTGACTTAAA AAAGAAGAGA GGGAGACCTC CTAAGATGAG GGAGGCAATG 
GCTGAGATGC CCTTCATGCA CAGCCTTAGT TTTCCTCTTT CTAGTACTGG ATTCTATCCC 
TCCTATGGCA TGCCTTACTC TCCCTCACCC CTTACAGCCA CTCCCCTAGG ATTAGGTTAC 
TATGGAAGGT ACCCTCCCAC TCTCTATCCT CCTCCTCCTT CACCTTCTTT CACCCCCCCA 
CTTCCTCCTC CTTCCTATAT GCATGCTGGC CATTTACTTC TCAATCCAAC CAAATACCAT 
AAGAAAAAGC ATAAGCTACT TCGCCAGGAG GCCTTTCTAA CAACCAGCAG GACTCCCCTC 
CTTTCCATGA GTACCTACCC TAGTGTTCCT CCTGAGATGG CCTATGGTTG GATGGTTGAG 
CACAAACATA GGCATCGTCA CAAGCACAGA GAACACCGTT CTTCTGAGCA ACCCCAGGTT 
TCCATGGATG CTGGCTCTTC CAGATCTGTT TTGGAGTCTT TGAAGCGCTA TCGATTTGGA 
AAGGAAACTG TTGGAGAGCG ATATAAACAT AAGGAAAAGC ACCGATGTCA TATGTCCTGC 
CCTCATCTCT CTCCTTCGAA AAGCTTAATA AATAGAGAAG AACAATGGGT CCACCGAGAG 
CCTTCAGAAT CTAGTCCATT GGCTTTGGGA TTGCAAACAC CTTTACAGAT TGACTGTTCC 
GAAAGTTCTC CAAGTTTATC TCTTGGAGGA TTCACTCCCA ACTCTGATCC GGCCAGCAGT 
GATGAACATA CAAACCTTTT CACAAGTGCA ATAGTGCAAG GCAGCTGCAG AGTTTCAAAC 
CCTAACTCCA GTGGCCGGAA GAAATTAACT GATAGCCCTG GACTGTTTTC TGCACAGGAC 
ACTTCACTAA TTCGGCCTCA CAGAAAGGAG CCACTGCCTT CCAGCGAAAG GGCAGTACAG 
ACCATGACAG GTTCCCAGCC AACCTCAGAT AAGTCTTCCC AGCGACCATC AGAGAGCACA 
AACTGTAGCC CTACCCGGAA GAGGTCTTCA TCTGAGAGTA CTTCTTCAAC AGTAAACGGA 
CTTCCTTCTC GAAGTCCAAG ATTGCTTGCC CCTGGGGATG ACTCTGTGGA TAGTCTGCTG 
CAGCGGATGG TACAACATGA GGACCAAGAG CCCCTGGAGA AGAATCTAGA TGCCGTGATT 
GCATCTGCCT CGGGGCCACC TTCTTCCAGT CCAGGCCACA GCCACAGCAA GGAACGAGCC 
CTGGGAAAAC CAGATAGCCT TCTAGTGCCC GCAGCCCCCA GTGACTCTTG TAGCAGCAGC 
ATCTCACTCC TGTCTGAGAA GTTGCCGAGC AGCTGCTCAC CCCACCATAT CAAGAGGAGT 
GTCGTGGAAG CCATGCAGCG CCAAGCTCGG AAAATGTGCA ATTATGACAA AATCTTGGCC 
ACAAAGAAAA ACCTGGACCA TGTCAATAAA ATATTAAAAG CCAAAAAGCT TCAAAGACAG 
GCCAGGACAG GGAATAACTT CGTGAAACGC AGACCAGGGC GACCTCGGAA ATGCCCCCTC 
CAGGCAGTGG TGTCAATGCA AGCATTCCAG GCTGCCCGAT TCGTCAGCCC CGATGCTAAT 
GAAGGTGAAG AAAGAGCAGC CCTGCCCCTC CGTCCAGACA CAGTTACGGA TGTCATCGAG 
GCTGTTGTGC AGAGCGTAAA CCTGAGTCCA GAACATAAGA AGGGGCTGAA GAGGAAAAGT 
TGGCTGTTGG AAGAACAGAC CAAGAAAAAG CAGAAGCCAT TCCCAGAGGA AGAACAAGAG 
CATCCCAAGA GCTTTACTGA AGCAGCAGTT GAGATTCCCA GTCCTCCTGA AACCCCAGCC 
AAGCCTTCCG AACCTGAAAG TACCTTGCAG CCTGTGCTTT CTCTCATCCC AAGGGAAAAG 
AAGGCCCCAC GTCCCCCAAA GAAGAAGTAT CAGAAAGCAG GGCTGTATTC TGACGTTTAC 
AAAACTACAG ACCCAAAGAG CCGATTGATC CAATTAAAGA AAGAAAAGTT GGAATACACT 
CCAGGAGAGC ATGAGTATGG ATTATTCCCA GCCCCTATTC ATGTTGGAAA GTATCTAAGG 
CAAAAGAGAA TTGACTTCCA GCTTCCCTAT GACATCCTTT GGCAGTGGAA ACATAATCAG 
CTATACAAAA AGCCAGATGT CCCACTATAT AAAAAAATCC GTTCAAACGT CTACGTGGAT 
GTCAAACCCC TTTCTGGTTA CGAGGCCACC ACCTGTAACT GTAAGAAGCC AGAGGATGAC 
ACCGGGAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCT 
AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGAGGCA TGAGTGGGTG 
CAGTGTCTAG AACGATTTCG AGCTGAGGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC 
CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGGGAGG TTGTCAGCGA ACAGGAGTTC 
AGGAACAGAA TGATTGAGCA GTATCATAAT CACAGTGACC ACTATTGCCT GAACCTGGAT 
AGTGGGATGG TGATTGACAG TTATCGGATG GGTAATGAGG CCCGATTCAT CAACCACAGC 
TGTGACCCAA ACTGTGAAAT GCAGAAATGG TCTGTCAATG GAGTATACCG GATTGGACTG 
TATGCTCTTA AAGATATGCC AGCTGGTACC GAGCTCACTT ATGATTACAA TTTCCATTCC 
TTTAATGTTG AGAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT 
GGGGGCAAGA GTCAGCGCGT GAACGGACTC GCCAGCAGCA AAAGCAGCCA GCCTGTGGCT 
ACCCATAAAA AGTCTGGACG GTCAAAAGAG AAGAGAAAGT CAAAGCACAA GCTGAAGAAA 
AGGAGAGGCC ACCTCTCCGA AGAACCCAGT GAAAACATCA ACACTCCAAC AAGATTGACC 
CCCCAATTAC AAATGAAGCC AATGTCTAAT CGAGAGAGGA ACTTCGTGTT AAAGCATCAT 
GTATTCTTGG TCCGAAACTG GGAGAAGATC CGACAGAAAC AAGAGGAAGT AAAGCATACC 
AGCGATAATC TCCACTCTGC ATCATTATAC ACCCGCTGGA ATGGCATCTG CCGAGATGAT 
GGGAACATCA AGTCTGACGT CTTCATGACC CAGTTCTCCG CCCTGCAGAC AGCACGATCA 
GTTCGAACAA GACGCCTGGC AGCAGCAGAG GAAAACATCG AGGTGGCACG GGCAGCCCGC 
CTCGCCCAAA TCTTCAAAGA GATCTGTGAT GGCATCATCT CCTACAAAGA TTCTTCCCGA 
CAAGCTTTGG CAGCACCCCT CTTGAACCTC CCCCCAAAGA AAAAGAATGC TGATTACTAT 
GAGAAGATCT CAGAGCCCCT GGACTTGGCC ACCATAGAGA GGCAGATCCT CACCGGCCAT 
TACAAGACCG TGGAGGCCTT TGACGCTGAC ATGCTCAAGG TGTTTCGGAA TGCTGAGAAG 
TACTACGGGC GTAAATCCCC AATTGGGAGA GATGTTTGCC GTCTACGGAA GACCTACTAC 
AACGCCAGGC ATGAGGCATC AGCCCAGATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG 
GCCGACAGCA GCGAAACATC TGTCTCTGAG AAGGAGAACG TGCATGAGAA GGATGACGAT 
GTGATCCGCT GTGTCTGTGG CCTCCACAAG GATGAGGGCC TCATGATCCA GTGTGACAAG 
TGCATGGTAT GGCAGCACTG TGACTGTATG GGGGTGAACT CAGACGTGGA GCATTACCTG 
TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC 
TATGCCCAGC CCGGCTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GTTGCTTCGT 
CAAGGTGACT GTGTGTACCT GATGCGAGAT AGCCGGCGCA CCCCTGATGG CCACCCAGTC 
CGCCAGTCCT ACCGACTGTT ATCACACATT AACCGAGATA AACTTGACAT CTTCCGCATT 
GAGAAGCTCT GGAAGAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA CTACTTCCGC 
CCCCACGAGA CCCACCACTC TCCGTCACGC CGCTTCTATC ACAATGAGCT ATTTAGGGTA 
CCACTCTATG AGATCATTCC CTTGGAGGCT GTGGTGGGGA CCTGCTGTGT ACTAGACCTT 
TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTATA CATCTGTGAC 
TATCGGCTTG ACAAGTCCGC ACACCTGTTT TACAAGATTC ACCGGAATCG CTATCCTGTC 
TGCACCAAAC CCTACGCCTT TGACCACTTC CCCAAGAAGC TCACTCCTAA AAGAGATTTC 
TCACCTCATT ATGTCCCAGA CAACTACAAG AGGAACGGAG GGCGGTCATC CTGGAAGTCT 
GAGCGCCCAA AGCCACCTCT AAAAGACCTG GGCCAGGAGG ATGACGCTCT GCCCTTGATT 
GAAGAGGTTC TGGCCAGCCA GGAGCAAGCC ACCAATGAGC TGCCCAGCCT GGAGGAGCCA 
GAACGGGAAG TGGCCCCTGT GGAAGTCAGC GAGGGTGAAA AGAAAGCAGA GGAAGGTGGT 
CCAGAATCTC AGGCTGCCTG TACCCCTGAG GAGCGACGGC ACAGCCAACG GGAACGGCTC 
AACCAGATCC TACTTGGCCT CCTGGAAAAA ATCCCTGGGA AAAATGCCAT CGATGTGACC 
TACTTGCTGG AGGAAGGGTC AGGCAGGAAG CTTCGAAGGC GCACTTTGTT CATCCCAGAG 
AACAGCTTTC GGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970812.1
CDS	390..9287
Translation	MDPRNTAMLGLGSDSEGFSRKSPSALGTGTVVSKREGELESNTKEEEDLRKRNWDRNSEAGKEDGLTDAQQQFSVKETNFSEGNLKLKIGLQAKRTKKPPKNLENYVCRPAIKTTIKHPRKALKGGKMTEEKDEHCPSRRDPSKLYKKTGDVAAVECQSEESIHLHSQGENSPLSKKLSPGHSEMTDYMNAASSTLVGSRDPDLKDRALLNGGTSVTEKLAQLIATCPPSKSSKTKPKKLGSGTSAGLVSKDLIRKAGVGSVAGIIHKDLIKKPAISTAVGLVTKDPGKKPVFNATVGLVNKDSVKKLGAGTTAVFINKDLGRKPGTVTTVGLLSKDSGKKLGIGIVPGLVNKESGKKLGLGTVVGLVNKDLGKKLSSTVGLVAKDCAKKIVANSTMGLVTKDMGKKLVSCSMAGLVNKDAINLKAEALLPTQEPLKASCSTNISSHENQELSESLKDSTTSKTFEKNVIRQSKESILEKFSVRKEIINLEKEMFNEGTCIQQDSFSSNDRGPYETSKHEKQPPVYCTSPDFQMGAASDASMAKSPFSAVGESNLPSPSPTVSVNPLTRSPPETSSQLAPNPLLLSPTTELMEDISESVGKNQFTSESTHLNIGHRSVGHSMNIECKGIDKELTDSKTAHIDIPRISSSLGKKPSLTSESSIHTITPSVVNFTSLFSNKPFLKLGAVSASDKHCQVAESLSSSLQSKPLKKRKGRKARWTKVVARSTCRSPKGLDLERSELFKSVSCSSLSNSNSEPAKFMKNIGPSSFVDHDFLKRRLPKLSKSTTPSLALLTDSEKPSHKAFATHKLSSSMCVSSDLLSDIYKPKRGRPKAKEMPQLEGPPKRTLKIPASKVFSLQSKEEQEPPILQPEIEIPSFKQSLSVSPFPKKRGRPKRQLRSPVKMKPPVLSVAPFVATESPSKLESESDNHRSSSDFFESEDQLQDPDDLDDSHRPAVCSMSDLEMEPDKKITKRNNGQLMKTIIRKINKMKTLKRKKLLNQILSSSVESSNKGKVQSKLHNTVSSLAATFGSKLGQQINVSKKGTIYIGKRRGRKPKTVLNGILSGSPTSLAVLEQTAQQAAGSALGQILPPLLPSSASSSEILPSPVCSQSSGTSGGQSPVSSDAGFVEPSSVPYLHLHSRQGSMIQTLAMKKASKGRRRLSPPTLLPNSPSHLSELTSLKEATPSPISESHSDETIPSDSGIGTDNNSTSDRAEKFCGQKKRRHSFEHVSLIPPETSTVLSSLKEKHKHKCKRRNHDYLSYDKMKRQKRKRKKKYPQLRSRQDPDFIADLEELISRLSEIRITHRSHHFIPRDLLPTIFRINFNSFYTHPSFPLDPLHYIRKPDLKKKRGRPPKMREAMAEMPFMHSLSFPLSSTGFYPSYGMPYSPSPLTATPLGLGYYGRYPPTLYPPPPSPSFTPPLPPPSYMHAGHLLLNPTKYHKKKHKLLRQEAFLTTSRTPLLSMSTYPSVPPEMAYGWMVEHKHRHRHKHREHRSSEQPQVSMDAGSSRSVLESLKRYRFGKETVGERYKHKEKHRCHMSCPHLSPSKSLINREEQWVHREPSESSPLALGLQTPLQIDCSESSPSLSLGGFTPNSDPASSDEHTNLFTSAIVQGSCRVSNPNSSGRKKLTDSPGLFSAQDTSLIRPHRKEPLPSSERAVQTMTGSQPTSDKSSQRPSESTNCSPTRKRSSSESTSSTVNGLPSRSPRLLAPGDDSVDSLLQRMVQHEDQEPLEKNLDAVIASASGPPSSSPGHSHSKERALGKPDSLLVPAAPSDSCSSSISLLSEKLPSSCSPHHIKRSVVEAMQRQARKMCNYDKILATKKNLDHVNKILKAKKLQRQARTGNNFVKRRPGRPRKCPLQAVVSMQAFQAARFVSPDANEGEERAALPLRPDTVTDVIEAVVQSVNLSPEHKKGLKRKSWLLEEQTKKKQKPFPEEEQEHPKSFTEAAVEIPSPPETPAKPSEPESTLQPVLSLIPREKKAPRPPKKKYQKAGLYSDVYKTTDPKSRLIQLKKEKLEYTPGEHEYGLFPAPIHVGKYLRQKRIDFQLPYDILWQWKHNQLYKKPDVPLYKKIRSNVYVDVKPLSGYEATTCNCKKPEDDTGKGCVDDCLNRMIFAECSPNTCPCGEQCCNQRIQRHEWVQCLERFRAEEKGWGIRTKEPLKAGQFIIEYLGEVVSEQEFRNRMIEQYHNHSDHYCLNLDSGMVIDSYRMGNEARFINHSCDPNCEMQKWSVNGVYRIGLYALKDMPAGTELTYDYNFHSFNVEKQQLCKCGFEKCRGIIGGKSQRVNGLASSKSSQPVATHKKSGRSKEKRKSKHKLKKRRGHLSEEPSENINTPTRLTPQLQMKPMSNRERNFVLKHHVFLVRNWEKIRQKQEEVKHTSDNLHSASLYTRWNGICRDDGNIKSDVFMTQFSALQTARSVRTRRLAAAEENIEVARAARLAQIFKEICDGIISYKDSSRQALAAPLLNLPPKKKNADYYEKISEPLDLATIERQILTGHYKTVEAFDADMLKVFRNAEKYYGRKSPIGRDVCRLRKTYYNARHEASAQIDEIVGETASEADSSETSVSEKENVHEKDDDVIRCVCGLHKDEGLMIQCDKCMVWQHCDCMGVNSDVEHYLCEQCDPRPVDREVPMIPRPHYAQPGCVYFICLLRDDLLLRQGDCVYLMRDSRRTPDGHPVRQSYRLLSHINRDKLDIFRIEKLWKNEKEERFAFGHHYFRPHETHHSPSRRFYHNELFRVPLYEIIPLEAVVGTCCVLDLYTYCKGRPKGVKEQDVYICDYRLDKSAHLFYKIHRNRYPVCTKPYAFDHFPKKLTPKRDFSPHYVPDNYKRNGGRSSWKSERPKPPLKDLGQEDDALPLIEEVLASQEQATNELPSLEEPEREVAPVEVSEGEKKAEEGGPESQAACTPEERRHSQRERLNQILLGLLEKIPGKNAIDVTYLLEEGSGRKLRRRTLFIPENSFRK

Target ORF information:

RefSeq Version	XM_014115337.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ash1 (absent, small, or homeotic)-like (Drosophila) (ASH1L), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115337.1

ORF Insert Sequence:

ATGGACCCTA GAAATACTGC TATGTTAGGA TTGGGTTCTG ACTCGGAAGG ATTTTCAAGG 
AAGAGTCCCT CTGCCCTTGG TACCGGCACA GTGGTCAGCA AGAGAGAAGG AGAGCTAGAG 
AGCAACACGA AAGAGGAAGA GGACCTTCGC AAGAGGAATT GGGACAGAAA CAGCGAAGCA 
GGGAAGGAGG ATGGTTTGAC TGATGCACAG CAACAGTTTT CAGTGAAAGA AACAAACTTT 
TCAGAGGGAA ACTTAAAATT GAAAATTGGC CTTCAGGCTA AGAGAACTAA AAAACCTCCA 
AAAAACTTGG AGAACTATGT ATGTCGACCT GCCATAAAAA CAACTATCAA GCACCCAAGG 
AAAGCACTTA AAGGTGGGAA GATGACAGAG GAGAAGGATG AGCACTGTCC TTCAAGACGG 
GACCCTTCAA AGTTGTACAA GAAAACAGGT GATGTTGCAG CCGTTGAATG CCAATCTGAA 
GAGAGCATCC ATCTTCATTC ACAGGGAGAA AACAGTCCTT TGTCTAAGAA GCTTTCTCCA 
GGACACTCAG AGATGACAGA TTACATGAAT GCAGCATCAT CTACTCTTGT TGGTAGCCGG 
GATCCTGATT TAAAGGATAG AGCATTACTT AATGGAGGAA CAAGTGTAAC AGAAAAGTTG 
GCACAGCTTA TTGCAACCTG TCCTCCCTCT AAGTCTTCCA AGACTAAACC GAAGAAGTTA 
GGAAGTGGCA CCAGCGCAGG ATTGGTTAGT AAGGATTTGA TCAGGAAAGC AGGTGTTGGC 
TCTGTAGCTG GAATAATACA TAAAGATTTA ATTAAAAAAC CAGCTATCAG CACAGCAGTT 
GGATTGGTAA CTAAAGATCC TGGGAAAAAG CCAGTGTTTA ATGCAACAGT AGGATTGGTC 
AATAAGGACT CTGTGAAAAA ACTAGGAGCT GGCACTACAG CGGTATTCAT TAATAAAGAC 
TTAGGCAGAA AGCCAGGGAC TGTCACTACA GTAGGACTGC TGAGCAAGGA TTCAGGAAAG 
AAGCTAGGAA TTGGTATTGT TCCAGGTTTA GTGAATAAGG AATCTGGAAA GAAGTTAGGA 
CTCGGCACTG TGGTCGGACT GGTTAATAAA GACTTGGGCA AGAAGTTGAG TTCTACTGTC 
GGTCTAGTGG CCAAGGACTG TGCAAAGAAG ATTGTAGCCA ATTCAACAAT GGGATTAGTT 
ACTAAAGACA TGGGAAAGAA ACTAGTGAGT TGTTCTATGG CAGGATTGGT CAATAAAGAT 
GCCATAAACC TTAAAGCCGA AGCACTGCTC CCCACTCAGG AACCACTTAA GGCTTCTTGT 
AGTACAAACA TCAGTAGTCA TGAAAATCAG GAACTTTCTG AATCCCTGAA AGATAGTACC 
ACCAGCAAAA CTTTTGAGAA GAATGTTATA CGGCAGAGTA AAGAAAGCAT ATTGGAAAAG 
TTCTCAGTTC GAAAAGAAAT CATTAATTTG GAGAAAGAAA TGTTTAACGA GGGAACATGC 
ATTCAGCAAG ACAGTTTCTC ATCCAATGAC AGGGGGCCTT ATGAAACCTC AAAGCATGAA 
AAGCAACCTC CCGTGTATTG CACTTCTCCA GACTTTCAAA TGGGAGCTGC TTCTGATGCA 
TCTATGGCAA AATCCCCATT TAGTGCAGTA GGAGAAAGCA ATCTCCCTTC CCCATCACCT 
ACTGTATCTG TTAATCCTTT AACCAGAAGT CCCCCTGAAA CTTCTTCACA GTTGGCTCCT 
AATCCGTTAC TTTTAAGTCC TACCACAGAA CTAATGGAAG ACATTTCTGA ATCCGTTGGG 
AAGAACCAGT TTACTTCTGA AAGTACCCAC TTGAACATTG GTCATAGGTC TGTGGGTCAC 
AGCATGAATA TTGAATGTAA AGGGATTGAT AAAGAGCTAA CTGATTCAAA AACTGCACAT 
ATAGATATTC CAAGAATAAG CTCTTCCCTG GGAAAAAAGC CAAGTTTGAC TTCTGAATCC 
AGTATTCACA CAATTACCCC TTCAGTTGTT AACTTCACTA GTTTATTTAG TAACAAGCCG 
TTTCTAAAAC TTGGTGCAGT ATCTGCTTCT GACAAACACT GCCAGGTTGC TGAAAGCCTA 
AGCTCTAGTT TGCAGTCCAA ACCATTAAAA AAAAGGAAAG GAAGAAAAGC TCGGTGGACT 
AAAGTGGTGG CAAGAAGCAC ATGCCGGTCT CCAAAAGGGC TAGATTTAGA AAGATCAGAG 
CTTTTTAAAA GTGTTTCATG TAGCTCACTA TCAAATAGTA ATTCTGAGCC AGCCAAGTTC 
ATGAAAAACA TTGGACCATC TTCATTTGTA GATCATGACT TCCTTAAACG CCGATTGCCA 
AAGTTGAGCA AGTCCACAAC TCCATCTCTT GCTCTCTTAA CTGATAGTGA AAAACCATCT 
CACAAGGCTT TTGCTACTCA CAAATTATCC TCCAGTATGT GTGTCTCTAG TGACCTTTTG 
TCTGATATTT ATAAGCCCAA AAGAGGAAGA CCTAAAGCTA AAGAGATGCC TCAACTGGAA 
GGTCCACCTA AAAGGACTTT AAAAATCCCT GCCTCTAAAG TTTTTTCTTT GCAGTCTAAG 
GAAGAACAAG AACCCCCAAT TTTACAGCCA GAAATTGAAA TTCCTTCCTT CAAACAAAGT 
CTTTCTGTAT CTCCTTTTCC AAAAAAGCGA GGCAGACCTA AGAGGCAGTT GAGGTCACCG 
GTCAAGATGA AGCCACCTGT ACTATCAGTG GCTCCATTTG TTGCCACGGA GAGTCCAAGC 
AAGCTGGAGT CTGAAAGTGA TAACCATCGA AGCAGCAGTG ATTTCTTTGA GAGTGAGGAT 
CAGCTTCAGG ACCCAGATGA TCTGGACGAC AGTCATAGGC CGGCTGTCTG CAGCATGAGT 
GACCTTGAGA TGGAACCAGA TAAAAAAATT ACAAAGAGAA ACAATGGACA ATTAATGAAA 
ACAATTATCC GCAAAATAAA TAAAATGAAG ACTTTAAAAA GGAAGAAACT GTTGAATCAG 
ATTCTTTCAA GCTCTGTAGA ATCAAGTAAT AAAGGGAAAG TGCAATCCAA ACTCCATAAT 
ACAGTGTCAA GTCTTGCTGC CACGTTTGGC TCTAAATTGG GCCAACAGAT AAATGTCAGC 
AAGAAAGGAA CCATTTACAT AGGAAAGAGA AGGGGTCGAA AACCAAAAAC TGTCTTAAAT 
GGCATTCTTT CTGGTAGCCC TACCAGCCTT GCTGTCCTTG AGCAAACTGC TCAACAGGCT 
GCCGGCTCAG CATTGGGACA GATACTTCCC CCTTTACTGC CTTCATCTGC TAGTAGCTCA 
GAGATTCTCC CATCGCCTGT TTGCTCTCAG TCTTCTGGGA CTAGTGGTGG TCAGAGCCCT 
GTAAGTAGTG ATGCAGGCTT TGTTGAACCC AGCTCAGTGC CATATTTGCA TTTACACTCC 
AGACAGGGCA GTATGATTCA GACTCTTGCG ATGAAAAAGG CCTCAAAGGG GAGGAGGCGA 
CTTTCTCCTC CTACTTTGTT GCCAAATTCT CCTTCACACT TGAGTGAACT CACCTCTCTG 
AAAGAAGCTA CTCCTTCTCC CATTAGTGAG TCTCATAGTG ATGAGACCAT TCCCAGTGAT 
AGTGGAATTG GAACAGATAA TAACAGCACA TCGGACAGAG CAGAGAAATT TTGTGGACAG 
AAAAAGAGGA GGCACTCTTT TGAACATGTT TCTCTGATTC CCCCTGAAAC CTCTACAGTC 
CTGAGCAGTC TTAAAGAAAA ACATAAACAT AAATGTAAGC GCCGGAATCA CGATTACCTC 
AGCTATGACA AGATGAAAAG GCAGAAACGA AAACGGAAAA AGAAATACCC CCAGCTCCGT 
AGCAGACAGG ATCCAGACTT TATTGCGGAT CTGGAGGAAT TGATAAGTCG GCTGAGTGAA 
ATTCGAATCA CCCATCGAAG CCATCATTTT ATCCCCCGAG ACCTGCTGCC AACTATTTTT 
CGAATCAACT TTAATAGTTT CTATACACAT CCTTCTTTCC CCTTAGACCC CTTGCACTAC 
ATTCGAAAAC CTGACTTAAA AAAGAAGAGA GGGAGACCTC CTAAGATGAG GGAGGCAATG 
GCTGAGATGC CCTTCATGCA CAGCCTTAGT TTTCCTCTTT CTAGTACTGG ATTCTATCCC 
TCCTATGGCA TGCCTTACTC TCCCTCACCC CTTACAGCCA CTCCCCTAGG ATTAGGTTAC 
TATGGAAGGT ACCCTCCCAC TCTCTATCCT CCTCCTCCTT CACCTTCTTT CACCCCCCCA 
CTTCCTCCTC CTTCCTATAT GCATGCTGGC CATTTACTTC TCAATCCAAC CAAATACCAT 
AAGAAAAAGC ATAAGCTACT TCGCCAGGAG GCCTTTCTAA CAACCAGCAG GACTCCCCTC 
CTTTCCATGA GTACCTACCC TAGTGTTCCT CCTGAGATGG CCTATGGTTG GATGGTTGAG 
CACAAACATA GGCATCGTCA CAAGCACAGA GAACACCGTT CTTCTGAGCA ACCCCAGGTT 
TCCATGGATG CTGGCTCTTC CAGATCTGTT TTGGAGTCTT TGAAGCGCTA TCGATTTGGA 
AAGGAAACTG TTGGAGAGCG ATATAAACAT AAGGAAAAGC ACCGATGTCA TATGTCCTGC 
CCTCATCTCT CTCCTTCGAA AAGCTTAATA AATAGAGAAG AACAATGGGT CCACCGAGAG 
CCTTCAGAAT CTAGTCCATT GGCTTTGGGA TTGCAAACAC CTTTACAGAT TGACTGTTCC 
GAAAGTTCTC CAAGTTTATC TCTTGGAGGA TTCACTCCCA ACTCTGATCC GGCCAGCAGT 
GATGAACATA CAAACCTTTT CACAAGTGCA ATAGTGCAAG GCAGCTGCAG AGTTTCAAAC 
CCTAACTCCA GTGGCCGGAA GAAATTAACT GATAGCCCTG GACTGTTTTC TGCACAGGAC 
ACTTCACTAA TTCGGCCTCA CAGAAAGGAG CCACTGCCTT CCAGCGAAAG GGCAGTACAG 
ACCATGACAG GTTCCCAGCC AACCTCAGAT AAGTCTTCCC AGCGACCATC AGAGAGCACA 
AACTGTAGCC CTACCCGGAA GAGGTCTTCA TCTGAGAGTA CTTCTTCAAC AGTAAACGGA 
CTTCCTTCTC GAAGTCCAAG ATTGCTTGCC CCTGGGGATG ACTCTGTGGA TAGTCTGCTG 
CAGCGGATGG TACAACATGA GGACCAAGAG CCCCTGGAGA AGAATCTAGA TGCCGTGATT 
GCATCTGCCT CGGGGCCACC TTCTTCCAGT CCAGGCCACA GCCACAGCAA GGAACGAGCC 
CTGGGAAAAC CAGATAGCCT TCTAGTGCCC GCAGCCCCCA GTGACTCTTG TAGCAGCAGC 
ATCTCACTCC TGTCTGAGAA GTTGCCGAGC AGCTGCTCAC CCCACCATAT CAAGAGGAGT 
GTCGTGGAAG CCATGCAGCG CCAAGCTCGG AAAATGTGCA ATTATGACAA AATCTTGGCC 
ACAAAGAAAA ACCTGGACCA TGTCAATAAA ATATTAAAAG CCAAAAAGCT TCAAAGACAG 
GCCAGGACAG GGAATAACTT CGTGAAACGC AGACCAGGGC GACCTCGGAA ATGCCCCCTC 
CAGGCAGTGG TGTCAATGCA AGCATTCCAG GCTGCCCGAT TCGTCAGCCC CGATGCTAAT 
GAAGGTGAAG AAAGAGCAGC CCTGCCCCTC CGTCCAGACA CAGTTACGGA TGTCATCGAG 
GCTGTTGTGC AGAGCGTAAA CCTGAGTCCA GAACATAAGA AGGGGCTGAA GAGGAAAAGT 
TGGCTGTTGG AAGAACAGAC CAAGAAAAAG CAGAAGCCAT TCCCAGAGGA AGAACAAGAG 
CATCCCAAGA GCTTTACTGA AGCAGCAGTT GAGATTCCCA GTCCTCCTGA AACCCCAGCC 
AAGCCTTCCG AACCTGAAAG TACCTTGCAG CCTGTGCTTT CTCTCATCCC AAGGGAAAAG 
AAGGCCCCAC GTCCCCCAAA GAAGAAGTAT CAGAAAGCAG GGCTGTATTC TGACGTTTAC 
AAAACTACAG ACCCAAAGAG CCGATTGATC CAATTAAAGA AAGAAAAGTT GGAATACACT 
CCAGGAGAGC ATGAGTATGG ATTATTCCCA GCCCCTATTC ATGTTGGAAA GTATCTAAGG 
CAAAAGAGAA TTGACTTCCA GCTTCCCTAT GACATCCTTT GGCAGTGGAA ACATAATCAG 
CTATACAAAA AGCCAGATGT CCCACTATAT AAAAAAATCC GTTCAAACGT CTACGTGGAT 
GTCAAACCCC TTTCTGGTTA CGAGGCCACC ACCTGTAACT GTAAGAAGCC AGAGGATGAC 
ACCGGGAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCT 
AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGAGGCA TGAGTGGGTG 
CAGTGTCTAG AACGATTTCG AGCTGAGGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC 
CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGGGAGG TTGTCAGCGA ACAGGAGTTC 
AGGAACAGAA TGATTGAGCA GTATCATAAT CACAGTGACC ACTATTGCCT GAACCTGGAT 
AGTGGGATGG TGATTGACAG TTATCGGATG GGTAATGAGG CCCGATTCAT CAACCACAGC 
TGTGACCCAA ACTGTGAAAT GCAGAAATGG TCTGTCAATG GAGTATACCG GATTGGACTG 
TATGCTCTTA AAGATATGCC AGCTGGTACC GAGCTCACTT ATGATTACAA TTTCCATTCC 
TTTAATGTTG AGAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT 
GGGGGCAAGA GTCAGCGCGT GAACGGACTC GCCAGCAGCA AAAGCAGCCA GCCTGTGGCT 
ACCCATAAAA AGTCTGGACG GTCAAAAGAG AAGAGAAAGT CAAAGCACAA GCTGAAGAAA 
AGGAGAGGCC ACCTCTCCGA AGAACCCAGT GAAAACATCA ACACTCCAAC AAGATTGACC 
CCCCAATTAC AAATGAAGCC AATGTCTAAT CGAGAGAGGA ACTTCGTGTT AAAGCATCAT 
GTATTCTTGG TCCGAAACTG GGAGAAGATC CGACAGAAAC AAGAGGAAGT AAAGCATACC 
AGCGATAATC TCCACTCTGC ATCATTATAC ACCCGCTGGA ATGGCATCTG CCGAGATGAT 
GGGAACATCA AGTCTGACGT CTTCATGACC CAGTTCTCCG CCCTGCAGAC AGCACGATCA 
GTTCGAACAA GACGCCTGGC AGCAGCAGAG GAAAACATCG AGGTGGCACG GGCAGCCCGC 
CTCGCCCAAA TCTTCAAAGA GATCTGTGAT GGCATCATCT CCTACAAAGA TTCTTCCCGA 
CAAGCTTTGG CAGCACCCCT CTTGAACCTC CCCCCAAAGA AAAAGAATGC TGATTACTAT 
GAGAAGATCT CAGAGCCCCT GGACTTGGCC ACCATAGAGA GGCAGATCCT CACCGGCCAT 
TACAAGACCG TGGAGGCCTT TGACGCTGAC ATGCTCAAGG TGTTTCGGAA TGCTGAGAAG 
TACTACGGGC GTAAATCCCC AATTGGGAGA GATGTTTGCC GTCTACGGAA GACCTACTAC 
AACGCCAGGC ATGAGGCATC AGCCCAGATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG 
GCCGACAGCA GCGAAACATC TGTCTCTGAG AAGGAGAACG TGCATGAGAA GGATGACGAT 
GTGATCCGCT GTGTCTGTGG CCTCCACAAG GATGAGGGCC TCATGATCCA GTGTGACAAG 
TGCATGGTAT GGCAGCACTG TGACTGTATG GGGGTGAACT CAGACGTGGA GCATTACCTG 
TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC 
TATGCCCAGC CCGGCTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GTTGCTTCGT 
CAAGGTGACT GTGTGTACCT GATGCGAGAT AGCCGGCGCA CCCCTGATGG CCACCCAGTC 
CGCCAGTCCT ACCGACTGTT ATCACACATT AACCGAGATA AACTTGACAT CTTCCGCATT 
GAGAAGCTCT GGAAGAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA CTACTTCCGC 
CCCCACGAGA CCCACCACTC TCCGTCACGC CGCTTCTATC ACAATGAGCT ATTTAGGGTA 
CCACTCTATG AGATCATTCC CTTGGAGGCT GTGGTGGGGA CCTGCTGTGT ACTAGACCTT 
TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTATA CATCTGTGAC 
TATCGGCTTG ACAAGTCCGC ACACCTGTTT TACAAGATTC ACCGGAATCG CTATCCTGTC 
TGCACCAAAC CCTACGCCTT TGACCACTTC CCCAAGAAGC TCACTCCTAA AAGAGATTTC 
TCACCTCATT ATGTCCCAGA CAACTACAAG AGGAACGGAG GGCGGTCATC CTGGAAGTCT 
GAGCGCCCAA AGCCACCTCT AAAAGACCTG GGCCAGGAGG ATGACGCTCT GCCCTTGATT 
GAAGAGGTTC TGGCCAGCCA GGAGCAAGCC ACCAATGAGC TGCCCAGCCT GGAGGAGCCA 
GAACGGGAAG TGGCCCCTGT GGAAGTCAGC GAGGGTGAAA AGAAAGCAGA GGAAGGTGGT 
CCAGAATCTC AGGCTGCCTG TACCCCTGAG GAGCGACGGC ACAGCCAACG GGAACGGCTC 
AACCAGATCC TACTTGGCCT CCTGGAAAAA ATCCCTGGGA AAAATGCCAT CGATGTGACC 
TACTTGCTGG AGGAAGGGTC AGGCAGGAAG CTTCGAAGGC GCACTTTGTT CATCCCAGAG 
AACAGCTTTC GGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASH1L ( XM_014115338.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013970813.1 Canis lupus familiaris ash1 (absent, small, or homeotic)-like (Drosophila) (ASH1L), transcript variant X4, mRNA.

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CloneID	OCe09533
Clone ID Related Accession (Same CDS sequence)	XM_014115336.2 , XM_537251.6 , XM_014115338.2 , XM_537251.5 , XM_014115337.1 , XM_014115338.1 , XM_014115336.1 , XM_014115337.2
Accession Version	XM_014115338.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8898bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	histone-lysine N-methyltransferase ASH1L
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCTA GAAATACTGC TATGTTAGGA TTGGGTTCTG ACTCGGAAGG ATTTTCAAGG 
AAGAGTCCCT CTGCCCTTGG TACCGGCACA GTGGTCAGCA AGAGAGAAGG AGAGCTAGAG 
AGCAACACGA AAGAGGAAGA GGACCTTCGC AAGAGGAATT GGGACAGAAA CAGCGAAGCA 
GGGAAGGAGG ATGGTTTGAC TGATGCACAG CAACAGTTTT CAGTGAAAGA AACAAACTTT 
TCAGAGGGAA ACTTAAAATT GAAAATTGGC CTTCAGGCTA AGAGAACTAA AAAACCTCCA 
AAAAACTTGG AGAACTATGT ATGTCGACCT GCCATAAAAA CAACTATCAA GCACCCAAGG 
AAAGCACTTA AAGGTGGGAA GATGACAGAG GAGAAGGATG AGCACTGTCC TTCAAGACGG 
GACCCTTCAA AGTTGTACAA GAAAACAGGT GATGTTGCAG CCGTTGAATG CCAATCTGAA 
GAGAGCATCC ATCTTCATTC ACAGGGAGAA AACAGTCCTT TGTCTAAGAA GCTTTCTCCA 
GGACACTCAG AGATGACAGA TTACATGAAT GCAGCATCAT CTACTCTTGT TGGTAGCCGG 
GATCCTGATT TAAAGGATAG AGCATTACTT AATGGAGGAA CAAGTGTAAC AGAAAAGTTG 
GCACAGCTTA TTGCAACCTG TCCTCCCTCT AAGTCTTCCA AGACTAAACC GAAGAAGTTA 
GGAAGTGGCA CCAGCGCAGG ATTGGTTAGT AAGGATTTGA TCAGGAAAGC AGGTGTTGGC 
TCTGTAGCTG GAATAATACA TAAAGATTTA ATTAAAAAAC CAGCTATCAG CACAGCAGTT 
GGATTGGTAA CTAAAGATCC TGGGAAAAAG CCAGTGTTTA ATGCAACAGT AGGATTGGTC 
AATAAGGACT CTGTGAAAAA ACTAGGAGCT GGCACTACAG CGGTATTCAT TAATAAAGAC 
TTAGGCAGAA AGCCAGGGAC TGTCACTACA GTAGGACTGC TGAGCAAGGA TTCAGGAAAG 
AAGCTAGGAA TTGGTATTGT TCCAGGTTTA GTGAATAAGG AATCTGGAAA GAAGTTAGGA 
CTCGGCACTG TGGTCGGACT GGTTAATAAA GACTTGGGCA AGAAGTTGAG TTCTACTGTC 
GGTCTAGTGG CCAAGGACTG TGCAAAGAAG ATTGTAGCCA ATTCAACAAT GGGATTAGTT 
ACTAAAGACA TGGGAAAGAA ACTAGTGAGT TGTTCTATGG CAGGATTGGT CAATAAAGAT 
GCCATAAACC TTAAAGCCGA AGCACTGCTC CCCACTCAGG AACCACTTAA GGCTTCTTGT 
AGTACAAACA TCAGTAGTCA TGAAAATCAG GAACTTTCTG AATCCCTGAA AGATAGTACC 
ACCAGCAAAA CTTTTGAGAA GAATGTTATA CGGCAGAGTA AAGAAAGCAT ATTGGAAAAG 
TTCTCAGTTC GAAAAGAAAT CATTAATTTG GAGAAAGAAA TGTTTAACGA GGGAACATGC 
ATTCAGCAAG ACAGTTTCTC ATCCAATGAC AGGGGGCCTT ATGAAACCTC AAAGCATGAA 
AAGCAACCTC CCGTGTATTG CACTTCTCCA GACTTTCAAA TGGGAGCTGC TTCTGATGCA 
TCTATGGCAA AATCCCCATT TAGTGCAGTA GGAGAAAGCA ATCTCCCTTC CCCATCACCT 
ACTGTATCTG TTAATCCTTT AACCAGAAGT CCCCCTGAAA CTTCTTCACA GTTGGCTCCT 
AATCCGTTAC TTTTAAGTCC TACCACAGAA CTAATGGAAG ACATTTCTGA ATCCGTTGGG 
AAGAACCAGT TTACTTCTGA AAGTACCCAC TTGAACATTG GTCATAGGTC TGTGGGTCAC 
AGCATGAATA TTGAATGTAA AGGGATTGAT AAAGAGCTAA CTGATTCAAA AACTGCACAT 
ATAGATATTC CAAGAATAAG CTCTTCCCTG GGAAAAAAGC CAAGTTTGAC TTCTGAATCC 
AGTATTCACA CAATTACCCC TTCAGTTGTT AACTTCACTA GTTTATTTAG TAACAAGCCG 
TTTCTAAAAC TTGGTGCAGT ATCTGCTTCT GACAAACACT GCCAGGTTGC TGAAAGCCTA 
AGCTCTAGTT TGCAGTCCAA ACCATTAAAA AAAAGGAAAG GAAGAAAAGC TCGGTGGACT 
AAAGTGGTGG CAAGAAGCAC ATGCCGGTCT CCAAAAGGGC TAGATTTAGA AAGATCAGAG 
CTTTTTAAAA GTGTTTCATG TAGCTCACTA TCAAATAGTA ATTCTGAGCC AGCCAAGTTC 
ATGAAAAACA TTGGACCATC TTCATTTGTA GATCATGACT TCCTTAAACG CCGATTGCCA 
AAGTTGAGCA AGTCCACAAC TCCATCTCTT GCTCTCTTAA CTGATAGTGA AAAACCATCT 
CACAAGGCTT TTGCTACTCA CAAATTATCC TCCAGTATGT GTGTCTCTAG TGACCTTTTG 
TCTGATATTT ATAAGCCCAA AAGAGGAAGA CCTAAAGCTA AAGAGATGCC TCAACTGGAA 
GGTCCACCTA AAAGGACTTT AAAAATCCCT GCCTCTAAAG TTTTTTCTTT GCAGTCTAAG 
GAAGAACAAG AACCCCCAAT TTTACAGCCA GAAATTGAAA TTCCTTCCTT CAAACAAAGT 
CTTTCTGTAT CTCCTTTTCC AAAAAAGCGA GGCAGACCTA AGAGGCAGTT GAGGTCACCG 
GTCAAGATGA AGCCACCTGT ACTATCAGTG GCTCCATTTG TTGCCACGGA GAGTCCAAGC 
AAGCTGGAGT CTGAAAGTGA TAACCATCGA AGCAGCAGTG ATTTCTTTGA GAGTGAGGAT 
CAGCTTCAGG ACCCAGATGA TCTGGACGAC AGTCATAGGC CGGCTGTCTG CAGCATGAGT 
GACCTTGAGA TGGAACCAGA TAAAAAAATT ACAAAGAGAA ACAATGGACA ATTAATGAAA 
ACAATTATCC GCAAAATAAA TAAAATGAAG ACTTTAAAAA GGAAGAAACT GTTGAATCAG 
ATTCTTTCAA GCTCTGTAGA ATCAAGTAAT AAAGGGAAAG TGCAATCCAA ACTCCATAAT 
ACAGTGTCAA GTCTTGCTGC CACGTTTGGC TCTAAATTGG GCCAACAGAT AAATGTCAGC 
AAGAAAGGAA CCATTTACAT AGGAAAGAGA AGGGGTCGAA AACCAAAAAC TGTCTTAAAT 
GGCATTCTTT CTGGTAGCCC TACCAGCCTT GCTGTCCTTG AGCAAACTGC TCAACAGGCT 
GCCGGCTCAG CATTGGGACA GATACTTCCC CCTTTACTGC CTTCATCTGC TAGTAGCTCA 
GAGATTCTCC CATCGCCTGT TTGCTCTCAG TCTTCTGGGA CTAGTGGTGG TCAGAGCCCT 
GTAAGTAGTG ATGCAGGCTT TGTTGAACCC AGCTCAGTGC CATATTTGCA TTTACACTCC 
AGACAGGGCA GTATGATTCA GACTCTTGCG ATGAAAAAGG CCTCAAAGGG GAGGAGGCGA 
CTTTCTCCTC CTACTTTGTT GCCAAATTCT CCTTCACACT TGAGTGAACT CACCTCTCTG 
AAAGAAGCTA CTCCTTCTCC CATTAGTGAG TCTCATAGTG ATGAGACCAT TCCCAGTGAT 
AGTGGAATTG GAACAGATAA TAACAGCACA TCGGACAGAG CAGAGAAATT TTGTGGACAG 
AAAAAGAGGA GGCACTCTTT TGAACATGTT TCTCTGATTC CCCCTGAAAC CTCTACAGTC 
CTGAGCAGTC TTAAAGAAAA ACATAAACAT AAATGTAAGC GCCGGAATCA CGATTACCTC 
AGCTATGACA AGATGAAAAG GCAGAAACGA AAACGGAAAA AGAAATACCC CCAGCTCCGT 
AGCAGACAGG ATCCAGACTT TATTGCGGAT CTGGAGGAAT TGATAAGTCG GCTGAGTGAA 
ATTCGAATCA CCCATCGAAG CCATCATTTT ATCCCCCGAG ACCTGCTGCC AACTATTTTT 
CGAATCAACT TTAATAGTTT CTATACACAT CCTTCTTTCC CCTTAGACCC CTTGCACTAC 
ATTCGAAAAC CTGACTTAAA AAAGAAGAGA GGGAGACCTC CTAAGATGAG GGAGGCAATG 
GCTGAGATGC CCTTCATGCA CAGCCTTAGT TTTCCTCTTT CTAGTACTGG ATTCTATCCC 
TCCTATGGCA TGCCTTACTC TCCCTCACCC CTTACAGCCA CTCCCCTAGG ATTAGGTTAC 
TATGGAAGGT ACCCTCCCAC TCTCTATCCT CCTCCTCCTT CACCTTCTTT CACCCCCCCA 
CTTCCTCCTC CTTCCTATAT GCATGCTGGC CATTTACTTC TCAATCCAAC CAAATACCAT 
AAGAAAAAGC ATAAGCTACT TCGCCAGGAG GCCTTTCTAA CAACCAGCAG GACTCCCCTC 
CTTTCCATGA GTACCTACCC TAGTGTTCCT CCTGAGATGG CCTATGGTTG GATGGTTGAG 
CACAAACATA GGCATCGTCA CAAGCACAGA GAACACCGTT CTTCTGAGCA ACCCCAGGTT 
TCCATGGATG CTGGCTCTTC CAGATCTGTT TTGGAGTCTT TGAAGCGCTA TCGATTTGGA 
AAGGAAACTG TTGGAGAGCG ATATAAACAT AAGGAAAAGC ACCGATGTCA TATGTCCTGC 
CCTCATCTCT CTCCTTCGAA AAGCTTAATA AATAGAGAAG AACAATGGGT CCACCGAGAG 
CCTTCAGAAT CTAGTCCATT GGCTTTGGGA TTGCAAACAC CTTTACAGAT TGACTGTTCC 
GAAAGTTCTC CAAGTTTATC TCTTGGAGGA TTCACTCCCA ACTCTGATCC GGCCAGCAGT 
GATGAACATA CAAACCTTTT CACAAGTGCA ATAGTGCAAG GCAGCTGCAG AGTTTCAAAC 
CCTAACTCCA GTGGCCGGAA GAAATTAACT GATAGCCCTG GACTGTTTTC TGCACAGGAC 
ACTTCACTAA TTCGGCCTCA CAGAAAGGAG CCACTGCCTT CCAGCGAAAG GGCAGTACAG 
ACCATGACAG GTTCCCAGCC AACCTCAGAT AAGTCTTCCC AGCGACCATC AGAGAGCACA 
AACTGTAGCC CTACCCGGAA GAGGTCTTCA TCTGAGAGTA CTTCTTCAAC AGTAAACGGA 
CTTCCTTCTC GAAGTCCAAG ATTGCTTGCC CCTGGGGATG ACTCTGTGGA TAGTCTGCTG 
CAGCGGATGG TACAACATGA GGACCAAGAG CCCCTGGAGA AGAATCTAGA TGCCGTGATT 
GCATCTGCCT CGGGGCCACC TTCTTCCAGT CCAGGCCACA GCCACAGCAA GGAACGAGCC 
CTGGGAAAAC CAGATAGCCT TCTAGTGCCC GCAGCCCCCA GTGACTCTTG TAGCAGCAGC 
ATCTCACTCC TGTCTGAGAA GTTGCCGAGC AGCTGCTCAC CCCACCATAT CAAGAGGAGT 
GTCGTGGAAG CCATGCAGCG CCAAGCTCGG AAAATGTGCA ATTATGACAA AATCTTGGCC 
ACAAAGAAAA ACCTGGACCA TGTCAATAAA ATATTAAAAG CCAAAAAGCT TCAAAGACAG 
GCCAGGACAG GGAATAACTT CGTGAAACGC AGACCAGGGC GACCTCGGAA ATGCCCCCTC 
CAGGCAGTGG TGTCAATGCA AGCATTCCAG GCTGCCCGAT TCGTCAGCCC CGATGCTAAT 
GAAGGTGAAG AAAGAGCAGC CCTGCCCCTC CGTCCAGACA CAGTTACGGA TGTCATCGAG 
GCTGTTGTGC AGAGCGTAAA CCTGAGTCCA GAACATAAGA AGGGGCTGAA GAGGAAAAGT 
TGGCTGTTGG AAGAACAGAC CAAGAAAAAG CAGAAGCCAT TCCCAGAGGA AGAACAAGAG 
CATCCCAAGA GCTTTACTGA AGCAGCAGTT GAGATTCCCA GTCCTCCTGA AACCCCAGCC 
AAGCCTTCCG AACCTGAAAG TACCTTGCAG CCTGTGCTTT CTCTCATCCC AAGGGAAAAG 
AAGGCCCCAC GTCCCCCAAA GAAGAAGTAT CAGAAAGCAG GGCTGTATTC TGACGTTTAC 
AAAACTACAG ACCCAAAGAG CCGATTGATC CAATTAAAGA AAGAAAAGTT GGAATACACT 
CCAGGAGAGC ATGAGTATGG ATTATTCCCA GCCCCTATTC ATGTTGGAAA GTATCTAAGG 
CAAAAGAGAA TTGACTTCCA GCTTCCCTAT GACATCCTTT GGCAGTGGAA ACATAATCAG 
CTATACAAAA AGCCAGATGT CCCACTATAT AAAAAAATCC GTTCAAACGT CTACGTGGAT 
GTCAAACCCC TTTCTGGTTA CGAGGCCACC ACCTGTAACT GTAAGAAGCC AGAGGATGAC 
ACCGGGAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCT 
AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGAGGCA TGAGTGGGTG 
CAGTGTCTAG AACGATTTCG AGCTGAGGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC 
CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGGGAGG TTGTCAGCGA ACAGGAGTTC 
AGGAACAGAA TGATTGAGCA GTATCATAAT CACAGTGACC ACTATTGCCT GAACCTGGAT 
AGTGGGATGG TGATTGACAG TTATCGGATG GGTAATGAGG CCCGATTCAT CAACCACAGC 
TGTGACCCAA ACTGTGAAAT GCAGAAATGG TCTGTCAATG GAGTATACCG GATTGGACTG 
TATGCTCTTA AAGATATGCC AGCTGGTACC GAGCTCACTT ATGATTACAA TTTCCATTCC 
TTTAATGTTG AGAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT 
GGGGGCAAGA GTCAGCGCGT GAACGGACTC GCCAGCAGCA AAAGCAGCCA GCCTGTGGCT 
ACCCATAAAA AGTCTGGACG GTCAAAAGAG AAGAGAAAGT CAAAGCACAA GCTGAAGAAA 
AGGAGAGGCC ACCTCTCCGA AGAACCCAGT GAAAACATCA ACACTCCAAC AAGATTGACC 
CCCCAATTAC AAATGAAGCC AATGTCTAAT CGAGAGAGGA ACTTCGTGTT AAAGCATCAT 
GTATTCTTGG TCCGAAACTG GGAGAAGATC CGACAGAAAC AAGAGGAAGT AAAGCATACC 
AGCGATAATC TCCACTCTGC ATCATTATAC ACCCGCTGGA ATGGCATCTG CCGAGATGAT 
GGGAACATCA AGTCTGACGT CTTCATGACC CAGTTCTCCG CCCTGCAGAC AGCACGATCA 
GTTCGAACAA GACGCCTGGC AGCAGCAGAG GAAAACATCG AGGTGGCACG GGCAGCCCGC 
CTCGCCCAAA TCTTCAAAGA GATCTGTGAT GGCATCATCT CCTACAAAGA TTCTTCCCGA 
CAAGCTTTGG CAGCACCCCT CTTGAACCTC CCCCCAAAGA AAAAGAATGC TGATTACTAT 
GAGAAGATCT CAGAGCCCCT GGACTTGGCC ACCATAGAGA GGCAGATCCT CACCGGCCAT 
TACAAGACCG TGGAGGCCTT TGACGCTGAC ATGCTCAAGG TGTTTCGGAA TGCTGAGAAG 
TACTACGGGC GTAAATCCCC AATTGGGAGA GATGTTTGCC GTCTACGGAA GACCTACTAC 
AACGCCAGGC ATGAGGCATC AGCCCAGATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG 
GCCGACAGCA GCGAAACATC TGTCTCTGAG AAGGAGAACG TGCATGAGAA GGATGACGAT 
GTGATCCGCT GTGTCTGTGG CCTCCACAAG GATGAGGGCC TCATGATCCA GTGTGACAAG 
TGCATGGTAT GGCAGCACTG TGACTGTATG GGGGTGAACT CAGACGTGGA GCATTACCTG 
TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC 
TATGCCCAGC CCGGCTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GTTGCTTCGT 
CAAGGTGACT GTGTGTACCT GATGCGAGAT AGCCGGCGCA CCCCTGATGG CCACCCAGTC 
CGCCAGTCCT ACCGACTGTT ATCACACATT AACCGAGATA AACTTGACAT CTTCCGCATT 
GAGAAGCTCT GGAAGAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA CTACTTCCGC 
CCCCACGAGA CCCACCACTC TCCGTCACGC CGCTTCTATC ACAATGAGCT ATTTAGGGTA 
CCACTCTATG AGATCATTCC CTTGGAGGCT GTGGTGGGGA CCTGCTGTGT ACTAGACCTT 
TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTATA CATCTGTGAC 
TATCGGCTTG ACAAGTCCGC ACACCTGTTT TACAAGATTC ACCGGAATCG CTATCCTGTC 
TGCACCAAAC CCTACGCCTT TGACCACTTC CCCAAGAAGC TCACTCCTAA AAGAGATTTC 
TCACCTCATT ATGTCCCAGA CAACTACAAG AGGAACGGAG GGCGGTCATC CTGGAAGTCT 
GAGCGCCCAA AGCCACCTCT AAAAGACCTG GGCCAGGAGG ATGACGCTCT GCCCTTGATT 
GAAGAGGTTC TGGCCAGCCA GGAGCAAGCC ACCAATGAGC TGCCCAGCCT GGAGGAGCCA 
GAACGGGAAG TGGCCCCTGT GGAAGTCAGC GAGGGTGAAA AGAAAGCAGA GGAAGGTGGT 
CCAGAATCTC AGGCTGCCTG TACCCCTGAG GAGCGACGGC ACAGCCAACG GGAACGGCTC 
AACCAGATCC TACTTGGCCT CCTGGAAAAA ATCCCTGGGA AAAATGCCAT CGATGTGACC 
TACTTGCTGG AGGAAGGGTC AGGCAGGAAG CTTCGAAGGC GCACTTTGTT CATCCCAGAG 
AACAGCTTTC GGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970813.1
CDS	143..9040
Translation	MDPRNTAMLGLGSDSEGFSRKSPSALGTGTVVSKREGELESNTKEEEDLRKRNWDRNSEAGKEDGLTDAQQQFSVKETNFSEGNLKLKIGLQAKRTKKPPKNLENYVCRPAIKTTIKHPRKALKGGKMTEEKDEHCPSRRDPSKLYKKTGDVAAVECQSEESIHLHSQGENSPLSKKLSPGHSEMTDYMNAASSTLVGSRDPDLKDRALLNGGTSVTEKLAQLIATCPPSKSSKTKPKKLGSGTSAGLVSKDLIRKAGVGSVAGIIHKDLIKKPAISTAVGLVTKDPGKKPVFNATVGLVNKDSVKKLGAGTTAVFINKDLGRKPGTVTTVGLLSKDSGKKLGIGIVPGLVNKESGKKLGLGTVVGLVNKDLGKKLSSTVGLVAKDCAKKIVANSTMGLVTKDMGKKLVSCSMAGLVNKDAINLKAEALLPTQEPLKASCSTNISSHENQELSESLKDSTTSKTFEKNVIRQSKESILEKFSVRKEIINLEKEMFNEGTCIQQDSFSSNDRGPYETSKHEKQPPVYCTSPDFQMGAASDASMAKSPFSAVGESNLPSPSPTVSVNPLTRSPPETSSQLAPNPLLLSPTTELMEDISESVGKNQFTSESTHLNIGHRSVGHSMNIECKGIDKELTDSKTAHIDIPRISSSLGKKPSLTSESSIHTITPSVVNFTSLFSNKPFLKLGAVSASDKHCQVAESLSSSLQSKPLKKRKGRKARWTKVVARSTCRSPKGLDLERSELFKSVSCSSLSNSNSEPAKFMKNIGPSSFVDHDFLKRRLPKLSKSTTPSLALLTDSEKPSHKAFATHKLSSSMCVSSDLLSDIYKPKRGRPKAKEMPQLEGPPKRTLKIPASKVFSLQSKEEQEPPILQPEIEIPSFKQSLSVSPFPKKRGRPKRQLRSPVKMKPPVLSVAPFVATESPSKLESESDNHRSSSDFFESEDQLQDPDDLDDSHRPAVCSMSDLEMEPDKKITKRNNGQLMKTIIRKINKMKTLKRKKLLNQILSSSVESSNKGKVQSKLHNTVSSLAATFGSKLGQQINVSKKGTIYIGKRRGRKPKTVLNGILSGSPTSLAVLEQTAQQAAGSALGQILPPLLPSSASSSEILPSPVCSQSSGTSGGQSPVSSDAGFVEPSSVPYLHLHSRQGSMIQTLAMKKASKGRRRLSPPTLLPNSPSHLSELTSLKEATPSPISESHSDETIPSDSGIGTDNNSTSDRAEKFCGQKKRRHSFEHVSLIPPETSTVLSSLKEKHKHKCKRRNHDYLSYDKMKRQKRKRKKKYPQLRSRQDPDFIADLEELISRLSEIRITHRSHHFIPRDLLPTIFRINFNSFYTHPSFPLDPLHYIRKPDLKKKRGRPPKMREAMAEMPFMHSLSFPLSSTGFYPSYGMPYSPSPLTATPLGLGYYGRYPPTLYPPPPSPSFTPPLPPPSYMHAGHLLLNPTKYHKKKHKLLRQEAFLTTSRTPLLSMSTYPSVPPEMAYGWMVEHKHRHRHKHREHRSSEQPQVSMDAGSSRSVLESLKRYRFGKETVGERYKHKEKHRCHMSCPHLSPSKSLINREEQWVHREPSESSPLALGLQTPLQIDCSESSPSLSLGGFTPNSDPASSDEHTNLFTSAIVQGSCRVSNPNSSGRKKLTDSPGLFSAQDTSLIRPHRKEPLPSSERAVQTMTGSQPTSDKSSQRPSESTNCSPTRKRSSSESTSSTVNGLPSRSPRLLAPGDDSVDSLLQRMVQHEDQEPLEKNLDAVIASASGPPSSSPGHSHSKERALGKPDSLLVPAAPSDSCSSSISLLSEKLPSSCSPHHIKRSVVEAMQRQARKMCNYDKILATKKNLDHVNKILKAKKLQRQARTGNNFVKRRPGRPRKCPLQAVVSMQAFQAARFVSPDANEGEERAALPLRPDTVTDVIEAVVQSVNLSPEHKKGLKRKSWLLEEQTKKKQKPFPEEEQEHPKSFTEAAVEIPSPPETPAKPSEPESTLQPVLSLIPREKKAPRPPKKKYQKAGLYSDVYKTTDPKSRLIQLKKEKLEYTPGEHEYGLFPAPIHVGKYLRQKRIDFQLPYDILWQWKHNQLYKKPDVPLYKKIRSNVYVDVKPLSGYEATTCNCKKPEDDTGKGCVDDCLNRMIFAECSPNTCPCGEQCCNQRIQRHEWVQCLERFRAEEKGWGIRTKEPLKAGQFIIEYLGEVVSEQEFRNRMIEQYHNHSDHYCLNLDSGMVIDSYRMGNEARFINHSCDPNCEMQKWSVNGVYRIGLYALKDMPAGTELTYDYNFHSFNVEKQQLCKCGFEKCRGIIGGKSQRVNGLASSKSSQPVATHKKSGRSKEKRKSKHKLKKRRGHLSEEPSENINTPTRLTPQLQMKPMSNRERNFVLKHHVFLVRNWEKIRQKQEEVKHTSDNLHSASLYTRWNGICRDDGNIKSDVFMTQFSALQTARSVRTRRLAAAEENIEVARAARLAQIFKEICDGIISYKDSSRQALAAPLLNLPPKKKNADYYEKISEPLDLATIERQILTGHYKTVEAFDADMLKVFRNAEKYYGRKSPIGRDVCRLRKTYYNARHEASAQIDEIVGETASEADSSETSVSEKENVHEKDDDVIRCVCGLHKDEGLMIQCDKCMVWQHCDCMGVNSDVEHYLCEQCDPRPVDREVPMIPRPHYAQPGCVYFICLLRDDLLLRQGDCVYLMRDSRRTPDGHPVRQSYRLLSHINRDKLDIFRIEKLWKNEKEERFAFGHHYFRPHETHHSPSRRFYHNELFRVPLYEIIPLEAVVGTCCVLDLYTYCKGRPKGVKEQDVYICDYRLDKSAHLFYKIHRNRYPVCTKPYAFDHFPKKLTPKRDFSPHYVPDNYKRNGGRSSWKSERPKPPLKDLGQEDDALPLIEEVLASQEQATNELPSLEEPEREVAPVEVSEGEKKAEEGGPESQAACTPEERRHSQRERLNQILLGLLEKIPGKNAIDVTYLLEEGSGRKLRRRTLFIPENSFRK

Target ORF information:

RefSeq Version	XM_014115338.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ash1 (absent, small, or homeotic)-like (Drosophila) (ASH1L), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115338.1

ORF Insert Sequence:

ATGGACCCTA GAAATACTGC TATGTTAGGA TTGGGTTCTG ACTCGGAAGG ATTTTCAAGG 
AAGAGTCCCT CTGCCCTTGG TACCGGCACA GTGGTCAGCA AGAGAGAAGG AGAGCTAGAG 
AGCAACACGA AAGAGGAAGA GGACCTTCGC AAGAGGAATT GGGACAGAAA CAGCGAAGCA 
GGGAAGGAGG ATGGTTTGAC TGATGCACAG CAACAGTTTT CAGTGAAAGA AACAAACTTT 
TCAGAGGGAA ACTTAAAATT GAAAATTGGC CTTCAGGCTA AGAGAACTAA AAAACCTCCA 
AAAAACTTGG AGAACTATGT ATGTCGACCT GCCATAAAAA CAACTATCAA GCACCCAAGG 
AAAGCACTTA AAGGTGGGAA GATGACAGAG GAGAAGGATG AGCACTGTCC TTCAAGACGG 
GACCCTTCAA AGTTGTACAA GAAAACAGGT GATGTTGCAG CCGTTGAATG CCAATCTGAA 
GAGAGCATCC ATCTTCATTC ACAGGGAGAA AACAGTCCTT TGTCTAAGAA GCTTTCTCCA 
GGACACTCAG AGATGACAGA TTACATGAAT GCAGCATCAT CTACTCTTGT TGGTAGCCGG 
GATCCTGATT TAAAGGATAG AGCATTACTT AATGGAGGAA CAAGTGTAAC AGAAAAGTTG 
GCACAGCTTA TTGCAACCTG TCCTCCCTCT AAGTCTTCCA AGACTAAACC GAAGAAGTTA 
GGAAGTGGCA CCAGCGCAGG ATTGGTTAGT AAGGATTTGA TCAGGAAAGC AGGTGTTGGC 
TCTGTAGCTG GAATAATACA TAAAGATTTA ATTAAAAAAC CAGCTATCAG CACAGCAGTT 
GGATTGGTAA CTAAAGATCC TGGGAAAAAG CCAGTGTTTA ATGCAACAGT AGGATTGGTC 
AATAAGGACT CTGTGAAAAA ACTAGGAGCT GGCACTACAG CGGTATTCAT TAATAAAGAC 
TTAGGCAGAA AGCCAGGGAC TGTCACTACA GTAGGACTGC TGAGCAAGGA TTCAGGAAAG 
AAGCTAGGAA TTGGTATTGT TCCAGGTTTA GTGAATAAGG AATCTGGAAA GAAGTTAGGA 
CTCGGCACTG TGGTCGGACT GGTTAATAAA GACTTGGGCA AGAAGTTGAG TTCTACTGTC 
GGTCTAGTGG CCAAGGACTG TGCAAAGAAG ATTGTAGCCA ATTCAACAAT GGGATTAGTT 
ACTAAAGACA TGGGAAAGAA ACTAGTGAGT TGTTCTATGG CAGGATTGGT CAATAAAGAT 
GCCATAAACC TTAAAGCCGA AGCACTGCTC CCCACTCAGG AACCACTTAA GGCTTCTTGT 
AGTACAAACA TCAGTAGTCA TGAAAATCAG GAACTTTCTG AATCCCTGAA AGATAGTACC 
ACCAGCAAAA CTTTTGAGAA GAATGTTATA CGGCAGAGTA AAGAAAGCAT ATTGGAAAAG 
TTCTCAGTTC GAAAAGAAAT CATTAATTTG GAGAAAGAAA TGTTTAACGA GGGAACATGC 
ATTCAGCAAG ACAGTTTCTC ATCCAATGAC AGGGGGCCTT ATGAAACCTC AAAGCATGAA 
AAGCAACCTC CCGTGTATTG CACTTCTCCA GACTTTCAAA TGGGAGCTGC TTCTGATGCA 
TCTATGGCAA AATCCCCATT TAGTGCAGTA GGAGAAAGCA ATCTCCCTTC CCCATCACCT 
ACTGTATCTG TTAATCCTTT AACCAGAAGT CCCCCTGAAA CTTCTTCACA GTTGGCTCCT 
AATCCGTTAC TTTTAAGTCC TACCACAGAA CTAATGGAAG ACATTTCTGA ATCCGTTGGG 
AAGAACCAGT TTACTTCTGA AAGTACCCAC TTGAACATTG GTCATAGGTC TGTGGGTCAC 
AGCATGAATA TTGAATGTAA AGGGATTGAT AAAGAGCTAA CTGATTCAAA AACTGCACAT 
ATAGATATTC CAAGAATAAG CTCTTCCCTG GGAAAAAAGC CAAGTTTGAC TTCTGAATCC 
AGTATTCACA CAATTACCCC TTCAGTTGTT AACTTCACTA GTTTATTTAG TAACAAGCCG 
TTTCTAAAAC TTGGTGCAGT ATCTGCTTCT GACAAACACT GCCAGGTTGC TGAAAGCCTA 
AGCTCTAGTT TGCAGTCCAA ACCATTAAAA AAAAGGAAAG GAAGAAAAGC TCGGTGGACT 
AAAGTGGTGG CAAGAAGCAC ATGCCGGTCT CCAAAAGGGC TAGATTTAGA AAGATCAGAG 
CTTTTTAAAA GTGTTTCATG TAGCTCACTA TCAAATAGTA ATTCTGAGCC AGCCAAGTTC 
ATGAAAAACA TTGGACCATC TTCATTTGTA GATCATGACT TCCTTAAACG CCGATTGCCA 
AAGTTGAGCA AGTCCACAAC TCCATCTCTT GCTCTCTTAA CTGATAGTGA AAAACCATCT 
CACAAGGCTT TTGCTACTCA CAAATTATCC TCCAGTATGT GTGTCTCTAG TGACCTTTTG 
TCTGATATTT ATAAGCCCAA AAGAGGAAGA CCTAAAGCTA AAGAGATGCC TCAACTGGAA 
GGTCCACCTA AAAGGACTTT AAAAATCCCT GCCTCTAAAG TTTTTTCTTT GCAGTCTAAG 
GAAGAACAAG AACCCCCAAT TTTACAGCCA GAAATTGAAA TTCCTTCCTT CAAACAAAGT 
CTTTCTGTAT CTCCTTTTCC AAAAAAGCGA GGCAGACCTA AGAGGCAGTT GAGGTCACCG 
GTCAAGATGA AGCCACCTGT ACTATCAGTG GCTCCATTTG TTGCCACGGA GAGTCCAAGC 
AAGCTGGAGT CTGAAAGTGA TAACCATCGA AGCAGCAGTG ATTTCTTTGA GAGTGAGGAT 
CAGCTTCAGG ACCCAGATGA TCTGGACGAC AGTCATAGGC CGGCTGTCTG CAGCATGAGT 
GACCTTGAGA TGGAACCAGA TAAAAAAATT ACAAAGAGAA ACAATGGACA ATTAATGAAA 
ACAATTATCC GCAAAATAAA TAAAATGAAG ACTTTAAAAA GGAAGAAACT GTTGAATCAG 
ATTCTTTCAA GCTCTGTAGA ATCAAGTAAT AAAGGGAAAG TGCAATCCAA ACTCCATAAT 
ACAGTGTCAA GTCTTGCTGC CACGTTTGGC TCTAAATTGG GCCAACAGAT AAATGTCAGC 
AAGAAAGGAA CCATTTACAT AGGAAAGAGA AGGGGTCGAA AACCAAAAAC TGTCTTAAAT 
GGCATTCTTT CTGGTAGCCC TACCAGCCTT GCTGTCCTTG AGCAAACTGC TCAACAGGCT 
GCCGGCTCAG CATTGGGACA GATACTTCCC CCTTTACTGC CTTCATCTGC TAGTAGCTCA 
GAGATTCTCC CATCGCCTGT TTGCTCTCAG TCTTCTGGGA CTAGTGGTGG TCAGAGCCCT 
GTAAGTAGTG ATGCAGGCTT TGTTGAACCC AGCTCAGTGC CATATTTGCA TTTACACTCC 
AGACAGGGCA GTATGATTCA GACTCTTGCG ATGAAAAAGG CCTCAAAGGG GAGGAGGCGA 
CTTTCTCCTC CTACTTTGTT GCCAAATTCT CCTTCACACT TGAGTGAACT CACCTCTCTG 
AAAGAAGCTA CTCCTTCTCC CATTAGTGAG TCTCATAGTG ATGAGACCAT TCCCAGTGAT 
AGTGGAATTG GAACAGATAA TAACAGCACA TCGGACAGAG CAGAGAAATT TTGTGGACAG 
AAAAAGAGGA GGCACTCTTT TGAACATGTT TCTCTGATTC CCCCTGAAAC CTCTACAGTC 
CTGAGCAGTC TTAAAGAAAA ACATAAACAT AAATGTAAGC GCCGGAATCA CGATTACCTC 
AGCTATGACA AGATGAAAAG GCAGAAACGA AAACGGAAAA AGAAATACCC CCAGCTCCGT 
AGCAGACAGG ATCCAGACTT TATTGCGGAT CTGGAGGAAT TGATAAGTCG GCTGAGTGAA 
ATTCGAATCA CCCATCGAAG CCATCATTTT ATCCCCCGAG ACCTGCTGCC AACTATTTTT 
CGAATCAACT TTAATAGTTT CTATACACAT CCTTCTTTCC CCTTAGACCC CTTGCACTAC 
ATTCGAAAAC CTGACTTAAA AAAGAAGAGA GGGAGACCTC CTAAGATGAG GGAGGCAATG 
GCTGAGATGC CCTTCATGCA CAGCCTTAGT TTTCCTCTTT CTAGTACTGG ATTCTATCCC 
TCCTATGGCA TGCCTTACTC TCCCTCACCC CTTACAGCCA CTCCCCTAGG ATTAGGTTAC 
TATGGAAGGT ACCCTCCCAC TCTCTATCCT CCTCCTCCTT CACCTTCTTT CACCCCCCCA 
CTTCCTCCTC CTTCCTATAT GCATGCTGGC CATTTACTTC TCAATCCAAC CAAATACCAT 
AAGAAAAAGC ATAAGCTACT TCGCCAGGAG GCCTTTCTAA CAACCAGCAG GACTCCCCTC 
CTTTCCATGA GTACCTACCC TAGTGTTCCT CCTGAGATGG CCTATGGTTG GATGGTTGAG 
CACAAACATA GGCATCGTCA CAAGCACAGA GAACACCGTT CTTCTGAGCA ACCCCAGGTT 
TCCATGGATG CTGGCTCTTC CAGATCTGTT TTGGAGTCTT TGAAGCGCTA TCGATTTGGA 
AAGGAAACTG TTGGAGAGCG ATATAAACAT AAGGAAAAGC ACCGATGTCA TATGTCCTGC 
CCTCATCTCT CTCCTTCGAA AAGCTTAATA AATAGAGAAG AACAATGGGT CCACCGAGAG 
CCTTCAGAAT CTAGTCCATT GGCTTTGGGA TTGCAAACAC CTTTACAGAT TGACTGTTCC 
GAAAGTTCTC CAAGTTTATC TCTTGGAGGA TTCACTCCCA ACTCTGATCC GGCCAGCAGT 
GATGAACATA CAAACCTTTT CACAAGTGCA ATAGTGCAAG GCAGCTGCAG AGTTTCAAAC 
CCTAACTCCA GTGGCCGGAA GAAATTAACT GATAGCCCTG GACTGTTTTC TGCACAGGAC 
ACTTCACTAA TTCGGCCTCA CAGAAAGGAG CCACTGCCTT CCAGCGAAAG GGCAGTACAG 
ACCATGACAG GTTCCCAGCC AACCTCAGAT AAGTCTTCCC AGCGACCATC AGAGAGCACA 
AACTGTAGCC CTACCCGGAA GAGGTCTTCA TCTGAGAGTA CTTCTTCAAC AGTAAACGGA 
CTTCCTTCTC GAAGTCCAAG ATTGCTTGCC CCTGGGGATG ACTCTGTGGA TAGTCTGCTG 
CAGCGGATGG TACAACATGA GGACCAAGAG CCCCTGGAGA AGAATCTAGA TGCCGTGATT 
GCATCTGCCT CGGGGCCACC TTCTTCCAGT CCAGGCCACA GCCACAGCAA GGAACGAGCC 
CTGGGAAAAC CAGATAGCCT TCTAGTGCCC GCAGCCCCCA GTGACTCTTG TAGCAGCAGC 
ATCTCACTCC TGTCTGAGAA GTTGCCGAGC AGCTGCTCAC CCCACCATAT CAAGAGGAGT 
GTCGTGGAAG CCATGCAGCG CCAAGCTCGG AAAATGTGCA ATTATGACAA AATCTTGGCC 
ACAAAGAAAA ACCTGGACCA TGTCAATAAA ATATTAAAAG CCAAAAAGCT TCAAAGACAG 
GCCAGGACAG GGAATAACTT CGTGAAACGC AGACCAGGGC GACCTCGGAA ATGCCCCCTC 
CAGGCAGTGG TGTCAATGCA AGCATTCCAG GCTGCCCGAT TCGTCAGCCC CGATGCTAAT 
GAAGGTGAAG AAAGAGCAGC CCTGCCCCTC CGTCCAGACA CAGTTACGGA TGTCATCGAG 
GCTGTTGTGC AGAGCGTAAA CCTGAGTCCA GAACATAAGA AGGGGCTGAA GAGGAAAAGT 
TGGCTGTTGG AAGAACAGAC CAAGAAAAAG CAGAAGCCAT TCCCAGAGGA AGAACAAGAG 
CATCCCAAGA GCTTTACTGA AGCAGCAGTT GAGATTCCCA GTCCTCCTGA AACCCCAGCC 
AAGCCTTCCG AACCTGAAAG TACCTTGCAG CCTGTGCTTT CTCTCATCCC AAGGGAAAAG 
AAGGCCCCAC GTCCCCCAAA GAAGAAGTAT CAGAAAGCAG GGCTGTATTC TGACGTTTAC 
AAAACTACAG ACCCAAAGAG CCGATTGATC CAATTAAAGA AAGAAAAGTT GGAATACACT 
CCAGGAGAGC ATGAGTATGG ATTATTCCCA GCCCCTATTC ATGTTGGAAA GTATCTAAGG 
CAAAAGAGAA TTGACTTCCA GCTTCCCTAT GACATCCTTT GGCAGTGGAA ACATAATCAG 
CTATACAAAA AGCCAGATGT CCCACTATAT AAAAAAATCC GTTCAAACGT CTACGTGGAT 
GTCAAACCCC TTTCTGGTTA CGAGGCCACC ACCTGTAACT GTAAGAAGCC AGAGGATGAC 
ACCGGGAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCT 
AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGAGGCA TGAGTGGGTG 
CAGTGTCTAG AACGATTTCG AGCTGAGGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC 
CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGGGAGG TTGTCAGCGA ACAGGAGTTC 
AGGAACAGAA TGATTGAGCA GTATCATAAT CACAGTGACC ACTATTGCCT GAACCTGGAT 
AGTGGGATGG TGATTGACAG TTATCGGATG GGTAATGAGG CCCGATTCAT CAACCACAGC 
TGTGACCCAA ACTGTGAAAT GCAGAAATGG TCTGTCAATG GAGTATACCG GATTGGACTG 
TATGCTCTTA AAGATATGCC AGCTGGTACC GAGCTCACTT ATGATTACAA TTTCCATTCC 
TTTAATGTTG AGAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT 
GGGGGCAAGA GTCAGCGCGT GAACGGACTC GCCAGCAGCA AAAGCAGCCA GCCTGTGGCT 
ACCCATAAAA AGTCTGGACG GTCAAAAGAG AAGAGAAAGT CAAAGCACAA GCTGAAGAAA 
AGGAGAGGCC ACCTCTCCGA AGAACCCAGT GAAAACATCA ACACTCCAAC AAGATTGACC 
CCCCAATTAC AAATGAAGCC AATGTCTAAT CGAGAGAGGA ACTTCGTGTT AAAGCATCAT 
GTATTCTTGG TCCGAAACTG GGAGAAGATC CGACAGAAAC AAGAGGAAGT AAAGCATACC 
AGCGATAATC TCCACTCTGC ATCATTATAC ACCCGCTGGA ATGGCATCTG CCGAGATGAT 
GGGAACATCA AGTCTGACGT CTTCATGACC CAGTTCTCCG CCCTGCAGAC AGCACGATCA 
GTTCGAACAA GACGCCTGGC AGCAGCAGAG GAAAACATCG AGGTGGCACG GGCAGCCCGC 
CTCGCCCAAA TCTTCAAAGA GATCTGTGAT GGCATCATCT CCTACAAAGA TTCTTCCCGA 
CAAGCTTTGG CAGCACCCCT CTTGAACCTC CCCCCAAAGA AAAAGAATGC TGATTACTAT 
GAGAAGATCT CAGAGCCCCT GGACTTGGCC ACCATAGAGA GGCAGATCCT CACCGGCCAT 
TACAAGACCG TGGAGGCCTT TGACGCTGAC ATGCTCAAGG TGTTTCGGAA TGCTGAGAAG 
TACTACGGGC GTAAATCCCC AATTGGGAGA GATGTTTGCC GTCTACGGAA GACCTACTAC 
AACGCCAGGC ATGAGGCATC AGCCCAGATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG 
GCCGACAGCA GCGAAACATC TGTCTCTGAG AAGGAGAACG TGCATGAGAA GGATGACGAT 
GTGATCCGCT GTGTCTGTGG CCTCCACAAG GATGAGGGCC TCATGATCCA GTGTGACAAG 
TGCATGGTAT GGCAGCACTG TGACTGTATG GGGGTGAACT CAGACGTGGA GCATTACCTG 
TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC 
TATGCCCAGC CCGGCTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GTTGCTTCGT 
CAAGGTGACT GTGTGTACCT GATGCGAGAT AGCCGGCGCA CCCCTGATGG CCACCCAGTC 
CGCCAGTCCT ACCGACTGTT ATCACACATT AACCGAGATA AACTTGACAT CTTCCGCATT 
GAGAAGCTCT GGAAGAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA CTACTTCCGC 
CCCCACGAGA CCCACCACTC TCCGTCACGC CGCTTCTATC ACAATGAGCT ATTTAGGGTA 
CCACTCTATG AGATCATTCC CTTGGAGGCT GTGGTGGGGA CCTGCTGTGT ACTAGACCTT 
TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTATA CATCTGTGAC 
TATCGGCTTG ACAAGTCCGC ACACCTGTTT TACAAGATTC ACCGGAATCG CTATCCTGTC 
TGCACCAAAC CCTACGCCTT TGACCACTTC CCCAAGAAGC TCACTCCTAA AAGAGATTTC 
TCACCTCATT ATGTCCCAGA CAACTACAAG AGGAACGGAG GGCGGTCATC CTGGAAGTCT 
GAGCGCCCAA AGCCACCTCT AAAAGACCTG GGCCAGGAGG ATGACGCTCT GCCCTTGATT 
GAAGAGGTTC TGGCCAGCCA GGAGCAAGCC ACCAATGAGC TGCCCAGCCT GGAGGAGCCA 
GAACGGGAAG TGGCCCCTGT GGAAGTCAGC GAGGGTGAAA AGAAAGCAGA GGAAGGTGGT 
CCAGAATCTC AGGCTGCCTG TACCCCTGAG GAGCGACGGC ACAGCCAACG GGAACGGCTC 
AACCAGATCC TACTTGGCCT CCTGGAAAAA ATCCCTGGGA AAAATGCCAT CGATGTGACC 
TACTTGCTGG AGGAAGGGTC AGGCAGGAAG CTTCGAAGGC GCACTTTGTT CATCCCAGAG 
AACAGCTTTC GGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASH1L ( XM_014115336.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013970811.1 Canis lupus familiaris ash1 (absent, small, or homeotic)-like (Drosophila) (ASH1L), transcript variant X2, mRNA.

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CloneID	OCe09533
Clone ID Related Accession (Same CDS sequence)	XM_014115336.2 , XM_537251.6 , XM_014115338.2 , XM_537251.5 , XM_014115337.1 , XM_014115338.1 , XM_014115336.1 , XM_014115337.2
Accession Version	XM_014115336.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8898bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	histone-lysine N-methyltransferase ASH1L
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCTA GAAATACTGC TATGTTAGGA TTGGGTTCTG ACTCGGAAGG ATTTTCAAGG 
AAGAGTCCCT CTGCCCTTGG TACCGGCACA GTGGTCAGCA AGAGAGAAGG AGAGCTAGAG 
AGCAACACGA AAGAGGAAGA GGACCTTCGC AAGAGGAATT GGGACAGAAA CAGCGAAGCA 
GGGAAGGAGG ATGGTTTGAC TGATGCACAG CAACAGTTTT CAGTGAAAGA AACAAACTTT 
TCAGAGGGAA ACTTAAAATT GAAAATTGGC CTTCAGGCTA AGAGAACTAA AAAACCTCCA 
AAAAACTTGG AGAACTATGT ATGTCGACCT GCCATAAAAA CAACTATCAA GCACCCAAGG 
AAAGCACTTA AAGGTGGGAA GATGACAGAG GAGAAGGATG AGCACTGTCC TTCAAGACGG 
GACCCTTCAA AGTTGTACAA GAAAACAGGT GATGTTGCAG CCGTTGAATG CCAATCTGAA 
GAGAGCATCC ATCTTCATTC ACAGGGAGAA AACAGTCCTT TGTCTAAGAA GCTTTCTCCA 
GGACACTCAG AGATGACAGA TTACATGAAT GCAGCATCAT CTACTCTTGT TGGTAGCCGG 
GATCCTGATT TAAAGGATAG AGCATTACTT AATGGAGGAA CAAGTGTAAC AGAAAAGTTG 
GCACAGCTTA TTGCAACCTG TCCTCCCTCT AAGTCTTCCA AGACTAAACC GAAGAAGTTA 
GGAAGTGGCA CCAGCGCAGG ATTGGTTAGT AAGGATTTGA TCAGGAAAGC AGGTGTTGGC 
TCTGTAGCTG GAATAATACA TAAAGATTTA ATTAAAAAAC CAGCTATCAG CACAGCAGTT 
GGATTGGTAA CTAAAGATCC TGGGAAAAAG CCAGTGTTTA ATGCAACAGT AGGATTGGTC 
AATAAGGACT CTGTGAAAAA ACTAGGAGCT GGCACTACAG CGGTATTCAT TAATAAAGAC 
TTAGGCAGAA AGCCAGGGAC TGTCACTACA GTAGGACTGC TGAGCAAGGA TTCAGGAAAG 
AAGCTAGGAA TTGGTATTGT TCCAGGTTTA GTGAATAAGG AATCTGGAAA GAAGTTAGGA 
CTCGGCACTG TGGTCGGACT GGTTAATAAA GACTTGGGCA AGAAGTTGAG TTCTACTGTC 
GGTCTAGTGG CCAAGGACTG TGCAAAGAAG ATTGTAGCCA ATTCAACAAT GGGATTAGTT 
ACTAAAGACA TGGGAAAGAA ACTAGTGAGT TGTTCTATGG CAGGATTGGT CAATAAAGAT 
GCCATAAACC TTAAAGCCGA AGCACTGCTC CCCACTCAGG AACCACTTAA GGCTTCTTGT 
AGTACAAACA TCAGTAGTCA TGAAAATCAG GAACTTTCTG AATCCCTGAA AGATAGTACC 
ACCAGCAAAA CTTTTGAGAA GAATGTTATA CGGCAGAGTA AAGAAAGCAT ATTGGAAAAG 
TTCTCAGTTC GAAAAGAAAT CATTAATTTG GAGAAAGAAA TGTTTAACGA GGGAACATGC 
ATTCAGCAAG ACAGTTTCTC ATCCAATGAC AGGGGGCCTT ATGAAACCTC AAAGCATGAA 
AAGCAACCTC CCGTGTATTG CACTTCTCCA GACTTTCAAA TGGGAGCTGC TTCTGATGCA 
TCTATGGCAA AATCCCCATT TAGTGCAGTA GGAGAAAGCA ATCTCCCTTC CCCATCACCT 
ACTGTATCTG TTAATCCTTT AACCAGAAGT CCCCCTGAAA CTTCTTCACA GTTGGCTCCT 
AATCCGTTAC TTTTAAGTCC TACCACAGAA CTAATGGAAG ACATTTCTGA ATCCGTTGGG 
AAGAACCAGT TTACTTCTGA AAGTACCCAC TTGAACATTG GTCATAGGTC TGTGGGTCAC 
AGCATGAATA TTGAATGTAA AGGGATTGAT AAAGAGCTAA CTGATTCAAA AACTGCACAT 
ATAGATATTC CAAGAATAAG CTCTTCCCTG GGAAAAAAGC CAAGTTTGAC TTCTGAATCC 
AGTATTCACA CAATTACCCC TTCAGTTGTT AACTTCACTA GTTTATTTAG TAACAAGCCG 
TTTCTAAAAC TTGGTGCAGT ATCTGCTTCT GACAAACACT GCCAGGTTGC TGAAAGCCTA 
AGCTCTAGTT TGCAGTCCAA ACCATTAAAA AAAAGGAAAG GAAGAAAAGC TCGGTGGACT 
AAAGTGGTGG CAAGAAGCAC ATGCCGGTCT CCAAAAGGGC TAGATTTAGA AAGATCAGAG 
CTTTTTAAAA GTGTTTCATG TAGCTCACTA TCAAATAGTA ATTCTGAGCC AGCCAAGTTC 
ATGAAAAACA TTGGACCATC TTCATTTGTA GATCATGACT TCCTTAAACG CCGATTGCCA 
AAGTTGAGCA AGTCCACAAC TCCATCTCTT GCTCTCTTAA CTGATAGTGA AAAACCATCT 
CACAAGGCTT TTGCTACTCA CAAATTATCC TCCAGTATGT GTGTCTCTAG TGACCTTTTG 
TCTGATATTT ATAAGCCCAA AAGAGGAAGA CCTAAAGCTA AAGAGATGCC TCAACTGGAA 
GGTCCACCTA AAAGGACTTT AAAAATCCCT GCCTCTAAAG TTTTTTCTTT GCAGTCTAAG 
GAAGAACAAG AACCCCCAAT TTTACAGCCA GAAATTGAAA TTCCTTCCTT CAAACAAAGT 
CTTTCTGTAT CTCCTTTTCC AAAAAAGCGA GGCAGACCTA AGAGGCAGTT GAGGTCACCG 
GTCAAGATGA AGCCACCTGT ACTATCAGTG GCTCCATTTG TTGCCACGGA GAGTCCAAGC 
AAGCTGGAGT CTGAAAGTGA TAACCATCGA AGCAGCAGTG ATTTCTTTGA GAGTGAGGAT 
CAGCTTCAGG ACCCAGATGA TCTGGACGAC AGTCATAGGC CGGCTGTCTG CAGCATGAGT 
GACCTTGAGA TGGAACCAGA TAAAAAAATT ACAAAGAGAA ACAATGGACA ATTAATGAAA 
ACAATTATCC GCAAAATAAA TAAAATGAAG ACTTTAAAAA GGAAGAAACT GTTGAATCAG 
ATTCTTTCAA GCTCTGTAGA ATCAAGTAAT AAAGGGAAAG TGCAATCCAA ACTCCATAAT 
ACAGTGTCAA GTCTTGCTGC CACGTTTGGC TCTAAATTGG GCCAACAGAT AAATGTCAGC 
AAGAAAGGAA CCATTTACAT AGGAAAGAGA AGGGGTCGAA AACCAAAAAC TGTCTTAAAT 
GGCATTCTTT CTGGTAGCCC TACCAGCCTT GCTGTCCTTG AGCAAACTGC TCAACAGGCT 
GCCGGCTCAG CATTGGGACA GATACTTCCC CCTTTACTGC CTTCATCTGC TAGTAGCTCA 
GAGATTCTCC CATCGCCTGT TTGCTCTCAG TCTTCTGGGA CTAGTGGTGG TCAGAGCCCT 
GTAAGTAGTG ATGCAGGCTT TGTTGAACCC AGCTCAGTGC CATATTTGCA TTTACACTCC 
AGACAGGGCA GTATGATTCA GACTCTTGCG ATGAAAAAGG CCTCAAAGGG GAGGAGGCGA 
CTTTCTCCTC CTACTTTGTT GCCAAATTCT CCTTCACACT TGAGTGAACT CACCTCTCTG 
AAAGAAGCTA CTCCTTCTCC CATTAGTGAG TCTCATAGTG ATGAGACCAT TCCCAGTGAT 
AGTGGAATTG GAACAGATAA TAACAGCACA TCGGACAGAG CAGAGAAATT TTGTGGACAG 
AAAAAGAGGA GGCACTCTTT TGAACATGTT TCTCTGATTC CCCCTGAAAC CTCTACAGTC 
CTGAGCAGTC TTAAAGAAAA ACATAAACAT AAATGTAAGC GCCGGAATCA CGATTACCTC 
AGCTATGACA AGATGAAAAG GCAGAAACGA AAACGGAAAA AGAAATACCC CCAGCTCCGT 
AGCAGACAGG ATCCAGACTT TATTGCGGAT CTGGAGGAAT TGATAAGTCG GCTGAGTGAA 
ATTCGAATCA CCCATCGAAG CCATCATTTT ATCCCCCGAG ACCTGCTGCC AACTATTTTT 
CGAATCAACT TTAATAGTTT CTATACACAT CCTTCTTTCC CCTTAGACCC CTTGCACTAC 
ATTCGAAAAC CTGACTTAAA AAAGAAGAGA GGGAGACCTC CTAAGATGAG GGAGGCAATG 
GCTGAGATGC CCTTCATGCA CAGCCTTAGT TTTCCTCTTT CTAGTACTGG ATTCTATCCC 
TCCTATGGCA TGCCTTACTC TCCCTCACCC CTTACAGCCA CTCCCCTAGG ATTAGGTTAC 
TATGGAAGGT ACCCTCCCAC TCTCTATCCT CCTCCTCCTT CACCTTCTTT CACCCCCCCA 
CTTCCTCCTC CTTCCTATAT GCATGCTGGC CATTTACTTC TCAATCCAAC CAAATACCAT 
AAGAAAAAGC ATAAGCTACT TCGCCAGGAG GCCTTTCTAA CAACCAGCAG GACTCCCCTC 
CTTTCCATGA GTACCTACCC TAGTGTTCCT CCTGAGATGG CCTATGGTTG GATGGTTGAG 
CACAAACATA GGCATCGTCA CAAGCACAGA GAACACCGTT CTTCTGAGCA ACCCCAGGTT 
TCCATGGATG CTGGCTCTTC CAGATCTGTT TTGGAGTCTT TGAAGCGCTA TCGATTTGGA 
AAGGAAACTG TTGGAGAGCG ATATAAACAT AAGGAAAAGC ACCGATGTCA TATGTCCTGC 
CCTCATCTCT CTCCTTCGAA AAGCTTAATA AATAGAGAAG AACAATGGGT CCACCGAGAG 
CCTTCAGAAT CTAGTCCATT GGCTTTGGGA TTGCAAACAC CTTTACAGAT TGACTGTTCC 
GAAAGTTCTC CAAGTTTATC TCTTGGAGGA TTCACTCCCA ACTCTGATCC GGCCAGCAGT 
GATGAACATA CAAACCTTTT CACAAGTGCA ATAGTGCAAG GCAGCTGCAG AGTTTCAAAC 
CCTAACTCCA GTGGCCGGAA GAAATTAACT GATAGCCCTG GACTGTTTTC TGCACAGGAC 
ACTTCACTAA TTCGGCCTCA CAGAAAGGAG CCACTGCCTT CCAGCGAAAG GGCAGTACAG 
ACCATGACAG GTTCCCAGCC AACCTCAGAT AAGTCTTCCC AGCGACCATC AGAGAGCACA 
AACTGTAGCC CTACCCGGAA GAGGTCTTCA TCTGAGAGTA CTTCTTCAAC AGTAAACGGA 
CTTCCTTCTC GAAGTCCAAG ATTGCTTGCC CCTGGGGATG ACTCTGTGGA TAGTCTGCTG 
CAGCGGATGG TACAACATGA GGACCAAGAG CCCCTGGAGA AGAATCTAGA TGCCGTGATT 
GCATCTGCCT CGGGGCCACC TTCTTCCAGT CCAGGCCACA GCCACAGCAA GGAACGAGCC 
CTGGGAAAAC CAGATAGCCT TCTAGTGCCC GCAGCCCCCA GTGACTCTTG TAGCAGCAGC 
ATCTCACTCC TGTCTGAGAA GTTGCCGAGC AGCTGCTCAC CCCACCATAT CAAGAGGAGT 
GTCGTGGAAG CCATGCAGCG CCAAGCTCGG AAAATGTGCA ATTATGACAA AATCTTGGCC 
ACAAAGAAAA ACCTGGACCA TGTCAATAAA ATATTAAAAG CCAAAAAGCT TCAAAGACAG 
GCCAGGACAG GGAATAACTT CGTGAAACGC AGACCAGGGC GACCTCGGAA ATGCCCCCTC 
CAGGCAGTGG TGTCAATGCA AGCATTCCAG GCTGCCCGAT TCGTCAGCCC CGATGCTAAT 
GAAGGTGAAG AAAGAGCAGC CCTGCCCCTC CGTCCAGACA CAGTTACGGA TGTCATCGAG 
GCTGTTGTGC AGAGCGTAAA CCTGAGTCCA GAACATAAGA AGGGGCTGAA GAGGAAAAGT 
TGGCTGTTGG AAGAACAGAC CAAGAAAAAG CAGAAGCCAT TCCCAGAGGA AGAACAAGAG 
CATCCCAAGA GCTTTACTGA AGCAGCAGTT GAGATTCCCA GTCCTCCTGA AACCCCAGCC 
AAGCCTTCCG AACCTGAAAG TACCTTGCAG CCTGTGCTTT CTCTCATCCC AAGGGAAAAG 
AAGGCCCCAC GTCCCCCAAA GAAGAAGTAT CAGAAAGCAG GGCTGTATTC TGACGTTTAC 
AAAACTACAG ACCCAAAGAG CCGATTGATC CAATTAAAGA AAGAAAAGTT GGAATACACT 
CCAGGAGAGC ATGAGTATGG ATTATTCCCA GCCCCTATTC ATGTTGGAAA GTATCTAAGG 
CAAAAGAGAA TTGACTTCCA GCTTCCCTAT GACATCCTTT GGCAGTGGAA ACATAATCAG 
CTATACAAAA AGCCAGATGT CCCACTATAT AAAAAAATCC GTTCAAACGT CTACGTGGAT 
GTCAAACCCC TTTCTGGTTA CGAGGCCACC ACCTGTAACT GTAAGAAGCC AGAGGATGAC 
ACCGGGAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCT 
AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGAGGCA TGAGTGGGTG 
CAGTGTCTAG AACGATTTCG AGCTGAGGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC 
CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGGGAGG TTGTCAGCGA ACAGGAGTTC 
AGGAACAGAA TGATTGAGCA GTATCATAAT CACAGTGACC ACTATTGCCT GAACCTGGAT 
AGTGGGATGG TGATTGACAG TTATCGGATG GGTAATGAGG CCCGATTCAT CAACCACAGC 
TGTGACCCAA ACTGTGAAAT GCAGAAATGG TCTGTCAATG GAGTATACCG GATTGGACTG 
TATGCTCTTA AAGATATGCC AGCTGGTACC GAGCTCACTT ATGATTACAA TTTCCATTCC 
TTTAATGTTG AGAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT 
GGGGGCAAGA GTCAGCGCGT GAACGGACTC GCCAGCAGCA AAAGCAGCCA GCCTGTGGCT 
ACCCATAAAA AGTCTGGACG GTCAAAAGAG AAGAGAAAGT CAAAGCACAA GCTGAAGAAA 
AGGAGAGGCC ACCTCTCCGA AGAACCCAGT GAAAACATCA ACACTCCAAC AAGATTGACC 
CCCCAATTAC AAATGAAGCC AATGTCTAAT CGAGAGAGGA ACTTCGTGTT AAAGCATCAT 
GTATTCTTGG TCCGAAACTG GGAGAAGATC CGACAGAAAC AAGAGGAAGT AAAGCATACC 
AGCGATAATC TCCACTCTGC ATCATTATAC ACCCGCTGGA ATGGCATCTG CCGAGATGAT 
GGGAACATCA AGTCTGACGT CTTCATGACC CAGTTCTCCG CCCTGCAGAC AGCACGATCA 
GTTCGAACAA GACGCCTGGC AGCAGCAGAG GAAAACATCG AGGTGGCACG GGCAGCCCGC 
CTCGCCCAAA TCTTCAAAGA GATCTGTGAT GGCATCATCT CCTACAAAGA TTCTTCCCGA 
CAAGCTTTGG CAGCACCCCT CTTGAACCTC CCCCCAAAGA AAAAGAATGC TGATTACTAT 
GAGAAGATCT CAGAGCCCCT GGACTTGGCC ACCATAGAGA GGCAGATCCT CACCGGCCAT 
TACAAGACCG TGGAGGCCTT TGACGCTGAC ATGCTCAAGG TGTTTCGGAA TGCTGAGAAG 
TACTACGGGC GTAAATCCCC AATTGGGAGA GATGTTTGCC GTCTACGGAA GACCTACTAC 
AACGCCAGGC ATGAGGCATC AGCCCAGATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG 
GCCGACAGCA GCGAAACATC TGTCTCTGAG AAGGAGAACG TGCATGAGAA GGATGACGAT 
GTGATCCGCT GTGTCTGTGG CCTCCACAAG GATGAGGGCC TCATGATCCA GTGTGACAAG 
TGCATGGTAT GGCAGCACTG TGACTGTATG GGGGTGAACT CAGACGTGGA GCATTACCTG 
TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC 
TATGCCCAGC CCGGCTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GTTGCTTCGT 
CAAGGTGACT GTGTGTACCT GATGCGAGAT AGCCGGCGCA CCCCTGATGG CCACCCAGTC 
CGCCAGTCCT ACCGACTGTT ATCACACATT AACCGAGATA AACTTGACAT CTTCCGCATT 
GAGAAGCTCT GGAAGAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA CTACTTCCGC 
CCCCACGAGA CCCACCACTC TCCGTCACGC CGCTTCTATC ACAATGAGCT ATTTAGGGTA 
CCACTCTATG AGATCATTCC CTTGGAGGCT GTGGTGGGGA CCTGCTGTGT ACTAGACCTT 
TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTATA CATCTGTGAC 
TATCGGCTTG ACAAGTCCGC ACACCTGTTT TACAAGATTC ACCGGAATCG CTATCCTGTC 
TGCACCAAAC CCTACGCCTT TGACCACTTC CCCAAGAAGC TCACTCCTAA AAGAGATTTC 
TCACCTCATT ATGTCCCAGA CAACTACAAG AGGAACGGAG GGCGGTCATC CTGGAAGTCT 
GAGCGCCCAA AGCCACCTCT AAAAGACCTG GGCCAGGAGG ATGACGCTCT GCCCTTGATT 
GAAGAGGTTC TGGCCAGCCA GGAGCAAGCC ACCAATGAGC TGCCCAGCCT GGAGGAGCCA 
GAACGGGAAG TGGCCCCTGT GGAAGTCAGC GAGGGTGAAA AGAAAGCAGA GGAAGGTGGT 
CCAGAATCTC AGGCTGCCTG TACCCCTGAG GAGCGACGGC ACAGCCAACG GGAACGGCTC 
AACCAGATCC TACTTGGCCT CCTGGAAAAA ATCCCTGGGA AAAATGCCAT CGATGTGACC 
TACTTGCTGG AGGAAGGGTC AGGCAGGAAG CTTCGAAGGC GCACTTTGTT CATCCCAGAG 
AACAGCTTTC GGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970811.1
CDS	274..9171
Translation	MDPRNTAMLGLGSDSEGFSRKSPSALGTGTVVSKREGELESNTKEEEDLRKRNWDRNSEAGKEDGLTDAQQQFSVKETNFSEGNLKLKIGLQAKRTKKPPKNLENYVCRPAIKTTIKHPRKALKGGKMTEEKDEHCPSRRDPSKLYKKTGDVAAVECQSEESIHLHSQGENSPLSKKLSPGHSEMTDYMNAASSTLVGSRDPDLKDRALLNGGTSVTEKLAQLIATCPPSKSSKTKPKKLGSGTSAGLVSKDLIRKAGVGSVAGIIHKDLIKKPAISTAVGLVTKDPGKKPVFNATVGLVNKDSVKKLGAGTTAVFINKDLGRKPGTVTTVGLLSKDSGKKLGIGIVPGLVNKESGKKLGLGTVVGLVNKDLGKKLSSTVGLVAKDCAKKIVANSTMGLVTKDMGKKLVSCSMAGLVNKDAINLKAEALLPTQEPLKASCSTNISSHENQELSESLKDSTTSKTFEKNVIRQSKESILEKFSVRKEIINLEKEMFNEGTCIQQDSFSSNDRGPYETSKHEKQPPVYCTSPDFQMGAASDASMAKSPFSAVGESNLPSPSPTVSVNPLTRSPPETSSQLAPNPLLLSPTTELMEDISESVGKNQFTSESTHLNIGHRSVGHSMNIECKGIDKELTDSKTAHIDIPRISSSLGKKPSLTSESSIHTITPSVVNFTSLFSNKPFLKLGAVSASDKHCQVAESLSSSLQSKPLKKRKGRKARWTKVVARSTCRSPKGLDLERSELFKSVSCSSLSNSNSEPAKFMKNIGPSSFVDHDFLKRRLPKLSKSTTPSLALLTDSEKPSHKAFATHKLSSSMCVSSDLLSDIYKPKRGRPKAKEMPQLEGPPKRTLKIPASKVFSLQSKEEQEPPILQPEIEIPSFKQSLSVSPFPKKRGRPKRQLRSPVKMKPPVLSVAPFVATESPSKLESESDNHRSSSDFFESEDQLQDPDDLDDSHRPAVCSMSDLEMEPDKKITKRNNGQLMKTIIRKINKMKTLKRKKLLNQILSSSVESSNKGKVQSKLHNTVSSLAATFGSKLGQQINVSKKGTIYIGKRRGRKPKTVLNGILSGSPTSLAVLEQTAQQAAGSALGQILPPLLPSSASSSEILPSPVCSQSSGTSGGQSPVSSDAGFVEPSSVPYLHLHSRQGSMIQTLAMKKASKGRRRLSPPTLLPNSPSHLSELTSLKEATPSPISESHSDETIPSDSGIGTDNNSTSDRAEKFCGQKKRRHSFEHVSLIPPETSTVLSSLKEKHKHKCKRRNHDYLSYDKMKRQKRKRKKKYPQLRSRQDPDFIADLEELISRLSEIRITHRSHHFIPRDLLPTIFRINFNSFYTHPSFPLDPLHYIRKPDLKKKRGRPPKMREAMAEMPFMHSLSFPLSSTGFYPSYGMPYSPSPLTATPLGLGYYGRYPPTLYPPPPSPSFTPPLPPPSYMHAGHLLLNPTKYHKKKHKLLRQEAFLTTSRTPLLSMSTYPSVPPEMAYGWMVEHKHRHRHKHREHRSSEQPQVSMDAGSSRSVLESLKRYRFGKETVGERYKHKEKHRCHMSCPHLSPSKSLINREEQWVHREPSESSPLALGLQTPLQIDCSESSPSLSLGGFTPNSDPASSDEHTNLFTSAIVQGSCRVSNPNSSGRKKLTDSPGLFSAQDTSLIRPHRKEPLPSSERAVQTMTGSQPTSDKSSQRPSESTNCSPTRKRSSSESTSSTVNGLPSRSPRLLAPGDDSVDSLLQRMVQHEDQEPLEKNLDAVIASASGPPSSSPGHSHSKERALGKPDSLLVPAAPSDSCSSSISLLSEKLPSSCSPHHIKRSVVEAMQRQARKMCNYDKILATKKNLDHVNKILKAKKLQRQARTGNNFVKRRPGRPRKCPLQAVVSMQAFQAARFVSPDANEGEERAALPLRPDTVTDVIEAVVQSVNLSPEHKKGLKRKSWLLEEQTKKKQKPFPEEEQEHPKSFTEAAVEIPSPPETPAKPSEPESTLQPVLSLIPREKKAPRPPKKKYQKAGLYSDVYKTTDPKSRLIQLKKEKLEYTPGEHEYGLFPAPIHVGKYLRQKRIDFQLPYDILWQWKHNQLYKKPDVPLYKKIRSNVYVDVKPLSGYEATTCNCKKPEDDTGKGCVDDCLNRMIFAECSPNTCPCGEQCCNQRIQRHEWVQCLERFRAEEKGWGIRTKEPLKAGQFIIEYLGEVVSEQEFRNRMIEQYHNHSDHYCLNLDSGMVIDSYRMGNEARFINHSCDPNCEMQKWSVNGVYRIGLYALKDMPAGTELTYDYNFHSFNVEKQQLCKCGFEKCRGIIGGKSQRVNGLASSKSSQPVATHKKSGRSKEKRKSKHKLKKRRGHLSEEPSENINTPTRLTPQLQMKPMSNRERNFVLKHHVFLVRNWEKIRQKQEEVKHTSDNLHSASLYTRWNGICRDDGNIKSDVFMTQFSALQTARSVRTRRLAAAEENIEVARAARLAQIFKEICDGIISYKDSSRQALAAPLLNLPPKKKNADYYEKISEPLDLATIERQILTGHYKTVEAFDADMLKVFRNAEKYYGRKSPIGRDVCRLRKTYYNARHEASAQIDEIVGETASEADSSETSVSEKENVHEKDDDVIRCVCGLHKDEGLMIQCDKCMVWQHCDCMGVNSDVEHYLCEQCDPRPVDREVPMIPRPHYAQPGCVYFICLLRDDLLLRQGDCVYLMRDSRRTPDGHPVRQSYRLLSHINRDKLDIFRIEKLWKNEKEERFAFGHHYFRPHETHHSPSRRFYHNELFRVPLYEIIPLEAVVGTCCVLDLYTYCKGRPKGVKEQDVYICDYRLDKSAHLFYKIHRNRYPVCTKPYAFDHFPKKLTPKRDFSPHYVPDNYKRNGGRSSWKSERPKPPLKDLGQEDDALPLIEEVLASQEQATNELPSLEEPEREVAPVEVSEGEKKAEEGGPESQAACTPEERRHSQRERLNQILLGLLEKIPGKNAIDVTYLLEEGSGRKLRRRTLFIPENSFRK

Target ORF information:

RefSeq Version	XM_014115336.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ash1 (absent, small, or homeotic)-like (Drosophila) (ASH1L), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115336.1

ORF Insert Sequence:

ATGGACCCTA GAAATACTGC TATGTTAGGA TTGGGTTCTG ACTCGGAAGG ATTTTCAAGG 
AAGAGTCCCT CTGCCCTTGG TACCGGCACA GTGGTCAGCA AGAGAGAAGG AGAGCTAGAG 
AGCAACACGA AAGAGGAAGA GGACCTTCGC AAGAGGAATT GGGACAGAAA CAGCGAAGCA 
GGGAAGGAGG ATGGTTTGAC TGATGCACAG CAACAGTTTT CAGTGAAAGA AACAAACTTT 
TCAGAGGGAA ACTTAAAATT GAAAATTGGC CTTCAGGCTA AGAGAACTAA AAAACCTCCA 
AAAAACTTGG AGAACTATGT ATGTCGACCT GCCATAAAAA CAACTATCAA GCACCCAAGG 
AAAGCACTTA AAGGTGGGAA GATGACAGAG GAGAAGGATG AGCACTGTCC TTCAAGACGG 
GACCCTTCAA AGTTGTACAA GAAAACAGGT GATGTTGCAG CCGTTGAATG CCAATCTGAA 
GAGAGCATCC ATCTTCATTC ACAGGGAGAA AACAGTCCTT TGTCTAAGAA GCTTTCTCCA 
GGACACTCAG AGATGACAGA TTACATGAAT GCAGCATCAT CTACTCTTGT TGGTAGCCGG 
GATCCTGATT TAAAGGATAG AGCATTACTT AATGGAGGAA CAAGTGTAAC AGAAAAGTTG 
GCACAGCTTA TTGCAACCTG TCCTCCCTCT AAGTCTTCCA AGACTAAACC GAAGAAGTTA 
GGAAGTGGCA CCAGCGCAGG ATTGGTTAGT AAGGATTTGA TCAGGAAAGC AGGTGTTGGC 
TCTGTAGCTG GAATAATACA TAAAGATTTA ATTAAAAAAC CAGCTATCAG CACAGCAGTT 
GGATTGGTAA CTAAAGATCC TGGGAAAAAG CCAGTGTTTA ATGCAACAGT AGGATTGGTC 
AATAAGGACT CTGTGAAAAA ACTAGGAGCT GGCACTACAG CGGTATTCAT TAATAAAGAC 
TTAGGCAGAA AGCCAGGGAC TGTCACTACA GTAGGACTGC TGAGCAAGGA TTCAGGAAAG 
AAGCTAGGAA TTGGTATTGT TCCAGGTTTA GTGAATAAGG AATCTGGAAA GAAGTTAGGA 
CTCGGCACTG TGGTCGGACT GGTTAATAAA GACTTGGGCA AGAAGTTGAG TTCTACTGTC 
GGTCTAGTGG CCAAGGACTG TGCAAAGAAG ATTGTAGCCA ATTCAACAAT GGGATTAGTT 
ACTAAAGACA TGGGAAAGAA ACTAGTGAGT TGTTCTATGG CAGGATTGGT CAATAAAGAT 
GCCATAAACC TTAAAGCCGA AGCACTGCTC CCCACTCAGG AACCACTTAA GGCTTCTTGT 
AGTACAAACA TCAGTAGTCA TGAAAATCAG GAACTTTCTG AATCCCTGAA AGATAGTACC 
ACCAGCAAAA CTTTTGAGAA GAATGTTATA CGGCAGAGTA AAGAAAGCAT ATTGGAAAAG 
TTCTCAGTTC GAAAAGAAAT CATTAATTTG GAGAAAGAAA TGTTTAACGA GGGAACATGC 
ATTCAGCAAG ACAGTTTCTC ATCCAATGAC AGGGGGCCTT ATGAAACCTC AAAGCATGAA 
AAGCAACCTC CCGTGTATTG CACTTCTCCA GACTTTCAAA TGGGAGCTGC TTCTGATGCA 
TCTATGGCAA AATCCCCATT TAGTGCAGTA GGAGAAAGCA ATCTCCCTTC CCCATCACCT 
ACTGTATCTG TTAATCCTTT AACCAGAAGT CCCCCTGAAA CTTCTTCACA GTTGGCTCCT 
AATCCGTTAC TTTTAAGTCC TACCACAGAA CTAATGGAAG ACATTTCTGA ATCCGTTGGG 
AAGAACCAGT TTACTTCTGA AAGTACCCAC TTGAACATTG GTCATAGGTC TGTGGGTCAC 
AGCATGAATA TTGAATGTAA AGGGATTGAT AAAGAGCTAA CTGATTCAAA AACTGCACAT 
ATAGATATTC CAAGAATAAG CTCTTCCCTG GGAAAAAAGC CAAGTTTGAC TTCTGAATCC 
AGTATTCACA CAATTACCCC TTCAGTTGTT AACTTCACTA GTTTATTTAG TAACAAGCCG 
TTTCTAAAAC TTGGTGCAGT ATCTGCTTCT GACAAACACT GCCAGGTTGC TGAAAGCCTA 
AGCTCTAGTT TGCAGTCCAA ACCATTAAAA AAAAGGAAAG GAAGAAAAGC TCGGTGGACT 
AAAGTGGTGG CAAGAAGCAC ATGCCGGTCT CCAAAAGGGC TAGATTTAGA AAGATCAGAG 
CTTTTTAAAA GTGTTTCATG TAGCTCACTA TCAAATAGTA ATTCTGAGCC AGCCAAGTTC 
ATGAAAAACA TTGGACCATC TTCATTTGTA GATCATGACT TCCTTAAACG CCGATTGCCA 
AAGTTGAGCA AGTCCACAAC TCCATCTCTT GCTCTCTTAA CTGATAGTGA AAAACCATCT 
CACAAGGCTT TTGCTACTCA CAAATTATCC TCCAGTATGT GTGTCTCTAG TGACCTTTTG 
TCTGATATTT ATAAGCCCAA AAGAGGAAGA CCTAAAGCTA AAGAGATGCC TCAACTGGAA 
GGTCCACCTA AAAGGACTTT AAAAATCCCT GCCTCTAAAG TTTTTTCTTT GCAGTCTAAG 
GAAGAACAAG AACCCCCAAT TTTACAGCCA GAAATTGAAA TTCCTTCCTT CAAACAAAGT 
CTTTCTGTAT CTCCTTTTCC AAAAAAGCGA GGCAGACCTA AGAGGCAGTT GAGGTCACCG 
GTCAAGATGA AGCCACCTGT ACTATCAGTG GCTCCATTTG TTGCCACGGA GAGTCCAAGC 
AAGCTGGAGT CTGAAAGTGA TAACCATCGA AGCAGCAGTG ATTTCTTTGA GAGTGAGGAT 
CAGCTTCAGG ACCCAGATGA TCTGGACGAC AGTCATAGGC CGGCTGTCTG CAGCATGAGT 
GACCTTGAGA TGGAACCAGA TAAAAAAATT ACAAAGAGAA ACAATGGACA ATTAATGAAA 
ACAATTATCC GCAAAATAAA TAAAATGAAG ACTTTAAAAA GGAAGAAACT GTTGAATCAG 
ATTCTTTCAA GCTCTGTAGA ATCAAGTAAT AAAGGGAAAG TGCAATCCAA ACTCCATAAT 
ACAGTGTCAA GTCTTGCTGC CACGTTTGGC TCTAAATTGG GCCAACAGAT AAATGTCAGC 
AAGAAAGGAA CCATTTACAT AGGAAAGAGA AGGGGTCGAA AACCAAAAAC TGTCTTAAAT 
GGCATTCTTT CTGGTAGCCC TACCAGCCTT GCTGTCCTTG AGCAAACTGC TCAACAGGCT 
GCCGGCTCAG CATTGGGACA GATACTTCCC CCTTTACTGC CTTCATCTGC TAGTAGCTCA 
GAGATTCTCC CATCGCCTGT TTGCTCTCAG TCTTCTGGGA CTAGTGGTGG TCAGAGCCCT 
GTAAGTAGTG ATGCAGGCTT TGTTGAACCC AGCTCAGTGC CATATTTGCA TTTACACTCC 
AGACAGGGCA GTATGATTCA GACTCTTGCG ATGAAAAAGG CCTCAAAGGG GAGGAGGCGA 
CTTTCTCCTC CTACTTTGTT GCCAAATTCT CCTTCACACT TGAGTGAACT CACCTCTCTG 
AAAGAAGCTA CTCCTTCTCC CATTAGTGAG TCTCATAGTG ATGAGACCAT TCCCAGTGAT 
AGTGGAATTG GAACAGATAA TAACAGCACA TCGGACAGAG CAGAGAAATT TTGTGGACAG 
AAAAAGAGGA GGCACTCTTT TGAACATGTT TCTCTGATTC CCCCTGAAAC CTCTACAGTC 
CTGAGCAGTC TTAAAGAAAA ACATAAACAT AAATGTAAGC GCCGGAATCA CGATTACCTC 
AGCTATGACA AGATGAAAAG GCAGAAACGA AAACGGAAAA AGAAATACCC CCAGCTCCGT 
AGCAGACAGG ATCCAGACTT TATTGCGGAT CTGGAGGAAT TGATAAGTCG GCTGAGTGAA 
ATTCGAATCA CCCATCGAAG CCATCATTTT ATCCCCCGAG ACCTGCTGCC AACTATTTTT 
CGAATCAACT TTAATAGTTT CTATACACAT CCTTCTTTCC CCTTAGACCC CTTGCACTAC 
ATTCGAAAAC CTGACTTAAA AAAGAAGAGA GGGAGACCTC CTAAGATGAG GGAGGCAATG 
GCTGAGATGC CCTTCATGCA CAGCCTTAGT TTTCCTCTTT CTAGTACTGG ATTCTATCCC 
TCCTATGGCA TGCCTTACTC TCCCTCACCC CTTACAGCCA CTCCCCTAGG ATTAGGTTAC 
TATGGAAGGT ACCCTCCCAC TCTCTATCCT CCTCCTCCTT CACCTTCTTT CACCCCCCCA 
CTTCCTCCTC CTTCCTATAT GCATGCTGGC CATTTACTTC TCAATCCAAC CAAATACCAT 
AAGAAAAAGC ATAAGCTACT TCGCCAGGAG GCCTTTCTAA CAACCAGCAG GACTCCCCTC 
CTTTCCATGA GTACCTACCC TAGTGTTCCT CCTGAGATGG CCTATGGTTG GATGGTTGAG 
CACAAACATA GGCATCGTCA CAAGCACAGA GAACACCGTT CTTCTGAGCA ACCCCAGGTT 
TCCATGGATG CTGGCTCTTC CAGATCTGTT TTGGAGTCTT TGAAGCGCTA TCGATTTGGA 
AAGGAAACTG TTGGAGAGCG ATATAAACAT AAGGAAAAGC ACCGATGTCA TATGTCCTGC 
CCTCATCTCT CTCCTTCGAA AAGCTTAATA AATAGAGAAG AACAATGGGT CCACCGAGAG 
CCTTCAGAAT CTAGTCCATT GGCTTTGGGA TTGCAAACAC CTTTACAGAT TGACTGTTCC 
GAAAGTTCTC CAAGTTTATC TCTTGGAGGA TTCACTCCCA ACTCTGATCC GGCCAGCAGT 
GATGAACATA CAAACCTTTT CACAAGTGCA ATAGTGCAAG GCAGCTGCAG AGTTTCAAAC 
CCTAACTCCA GTGGCCGGAA GAAATTAACT GATAGCCCTG GACTGTTTTC TGCACAGGAC 
ACTTCACTAA TTCGGCCTCA CAGAAAGGAG CCACTGCCTT CCAGCGAAAG GGCAGTACAG 
ACCATGACAG GTTCCCAGCC AACCTCAGAT AAGTCTTCCC AGCGACCATC AGAGAGCACA 
AACTGTAGCC CTACCCGGAA GAGGTCTTCA TCTGAGAGTA CTTCTTCAAC AGTAAACGGA 
CTTCCTTCTC GAAGTCCAAG ATTGCTTGCC CCTGGGGATG ACTCTGTGGA TAGTCTGCTG 
CAGCGGATGG TACAACATGA GGACCAAGAG CCCCTGGAGA AGAATCTAGA TGCCGTGATT 
GCATCTGCCT CGGGGCCACC TTCTTCCAGT CCAGGCCACA GCCACAGCAA GGAACGAGCC 
CTGGGAAAAC CAGATAGCCT TCTAGTGCCC GCAGCCCCCA GTGACTCTTG TAGCAGCAGC 
ATCTCACTCC TGTCTGAGAA GTTGCCGAGC AGCTGCTCAC CCCACCATAT CAAGAGGAGT 
GTCGTGGAAG CCATGCAGCG CCAAGCTCGG AAAATGTGCA ATTATGACAA AATCTTGGCC 
ACAAAGAAAA ACCTGGACCA TGTCAATAAA ATATTAAAAG CCAAAAAGCT TCAAAGACAG 
GCCAGGACAG GGAATAACTT CGTGAAACGC AGACCAGGGC GACCTCGGAA ATGCCCCCTC 
CAGGCAGTGG TGTCAATGCA AGCATTCCAG GCTGCCCGAT TCGTCAGCCC CGATGCTAAT 
GAAGGTGAAG AAAGAGCAGC CCTGCCCCTC CGTCCAGACA CAGTTACGGA TGTCATCGAG 
GCTGTTGTGC AGAGCGTAAA CCTGAGTCCA GAACATAAGA AGGGGCTGAA GAGGAAAAGT 
TGGCTGTTGG AAGAACAGAC CAAGAAAAAG CAGAAGCCAT TCCCAGAGGA AGAACAAGAG 
CATCCCAAGA GCTTTACTGA AGCAGCAGTT GAGATTCCCA GTCCTCCTGA AACCCCAGCC 
AAGCCTTCCG AACCTGAAAG TACCTTGCAG CCTGTGCTTT CTCTCATCCC AAGGGAAAAG 
AAGGCCCCAC GTCCCCCAAA GAAGAAGTAT CAGAAAGCAG GGCTGTATTC TGACGTTTAC 
AAAACTACAG ACCCAAAGAG CCGATTGATC CAATTAAAGA AAGAAAAGTT GGAATACACT 
CCAGGAGAGC ATGAGTATGG ATTATTCCCA GCCCCTATTC ATGTTGGAAA GTATCTAAGG 
CAAAAGAGAA TTGACTTCCA GCTTCCCTAT GACATCCTTT GGCAGTGGAA ACATAATCAG 
CTATACAAAA AGCCAGATGT CCCACTATAT AAAAAAATCC GTTCAAACGT CTACGTGGAT 
GTCAAACCCC TTTCTGGTTA CGAGGCCACC ACCTGTAACT GTAAGAAGCC AGAGGATGAC 
ACCGGGAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCT 
AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGAGGCA TGAGTGGGTG 
CAGTGTCTAG AACGATTTCG AGCTGAGGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC 
CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGGGAGG TTGTCAGCGA ACAGGAGTTC 
AGGAACAGAA TGATTGAGCA GTATCATAAT CACAGTGACC ACTATTGCCT GAACCTGGAT 
AGTGGGATGG TGATTGACAG TTATCGGATG GGTAATGAGG CCCGATTCAT CAACCACAGC 
TGTGACCCAA ACTGTGAAAT GCAGAAATGG TCTGTCAATG GAGTATACCG GATTGGACTG 
TATGCTCTTA AAGATATGCC AGCTGGTACC GAGCTCACTT ATGATTACAA TTTCCATTCC 
TTTAATGTTG AGAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT 
GGGGGCAAGA GTCAGCGCGT GAACGGACTC GCCAGCAGCA AAAGCAGCCA GCCTGTGGCT 
ACCCATAAAA AGTCTGGACG GTCAAAAGAG AAGAGAAAGT CAAAGCACAA GCTGAAGAAA 
AGGAGAGGCC ACCTCTCCGA AGAACCCAGT GAAAACATCA ACACTCCAAC AAGATTGACC 
CCCCAATTAC AAATGAAGCC AATGTCTAAT CGAGAGAGGA ACTTCGTGTT AAAGCATCAT 
GTATTCTTGG TCCGAAACTG GGAGAAGATC CGACAGAAAC AAGAGGAAGT AAAGCATACC 
AGCGATAATC TCCACTCTGC ATCATTATAC ACCCGCTGGA ATGGCATCTG CCGAGATGAT 
GGGAACATCA AGTCTGACGT CTTCATGACC CAGTTCTCCG CCCTGCAGAC AGCACGATCA 
GTTCGAACAA GACGCCTGGC AGCAGCAGAG GAAAACATCG AGGTGGCACG GGCAGCCCGC 
CTCGCCCAAA TCTTCAAAGA GATCTGTGAT GGCATCATCT CCTACAAAGA TTCTTCCCGA 
CAAGCTTTGG CAGCACCCCT CTTGAACCTC CCCCCAAAGA AAAAGAATGC TGATTACTAT 
GAGAAGATCT CAGAGCCCCT GGACTTGGCC ACCATAGAGA GGCAGATCCT CACCGGCCAT 
TACAAGACCG TGGAGGCCTT TGACGCTGAC ATGCTCAAGG TGTTTCGGAA TGCTGAGAAG 
TACTACGGGC GTAAATCCCC AATTGGGAGA GATGTTTGCC GTCTACGGAA GACCTACTAC 
AACGCCAGGC ATGAGGCATC AGCCCAGATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG 
GCCGACAGCA GCGAAACATC TGTCTCTGAG AAGGAGAACG TGCATGAGAA GGATGACGAT 
GTGATCCGCT GTGTCTGTGG CCTCCACAAG GATGAGGGCC TCATGATCCA GTGTGACAAG 
TGCATGGTAT GGCAGCACTG TGACTGTATG GGGGTGAACT CAGACGTGGA GCATTACCTG 
TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC 
TATGCCCAGC CCGGCTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GTTGCTTCGT 
CAAGGTGACT GTGTGTACCT GATGCGAGAT AGCCGGCGCA CCCCTGATGG CCACCCAGTC 
CGCCAGTCCT ACCGACTGTT ATCACACATT AACCGAGATA AACTTGACAT CTTCCGCATT 
GAGAAGCTCT GGAAGAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA CTACTTCCGC 
CCCCACGAGA CCCACCACTC TCCGTCACGC CGCTTCTATC ACAATGAGCT ATTTAGGGTA 
CCACTCTATG AGATCATTCC CTTGGAGGCT GTGGTGGGGA CCTGCTGTGT ACTAGACCTT 
TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTATA CATCTGTGAC 
TATCGGCTTG ACAAGTCCGC ACACCTGTTT TACAAGATTC ACCGGAATCG CTATCCTGTC 
TGCACCAAAC CCTACGCCTT TGACCACTTC CCCAAGAAGC TCACTCCTAA AAGAGATTTC 
TCACCTCATT ATGTCCCAGA CAACTACAAG AGGAACGGAG GGCGGTCATC CTGGAAGTCT 
GAGCGCCCAA AGCCACCTCT AAAAGACCTG GGCCAGGAGG ATGACGCTCT GCCCTTGATT 
GAAGAGGTTC TGGCCAGCCA GGAGCAAGCC ACCAATGAGC TGCCCAGCCT GGAGGAGCCA 
GAACGGGAAG TGGCCCCTGT GGAAGTCAGC GAGGGTGAAA AGAAAGCAGA GGAAGGTGGT 
CCAGAATCTC AGGCTGCCTG TACCCCTGAG GAGCGACGGC ACAGCCAACG GGAACGGCTC 
AACCAGATCC TACTTGGCCT CCTGGAAAAA ATCCCTGGGA AAAATGCCAT CGATGTGACC 
TACTTGCTGG AGGAAGGGTC AGGCAGGAAG CTTCGAAGGC GCACTTTGTT CATCCCAGAG 
AACAGCTTTC GGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASH1L ( XM_014115337.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013970812.1 Canis lupus familiaris ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X4, mRNA.

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CloneID	OCe09533
Clone ID Related Accession (Same CDS sequence)	XM_014115336.2 , XM_537251.6 , XM_014115338.2 , XM_537251.5 , XM_014115337.1 , XM_014115338.1 , XM_014115336.1 , XM_014115337.2
Accession Version	XM_014115337.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8898bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	histone-lysine N-methyltransferase ASH1L
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014115337.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCTA GAAATACTGC TATGTTAGGA TTGGGTTCTG ACTCGGAAGG ATTTTCAAGG 
AAGAGTCCCT CTGCCCTTGG TACCGGCACA GTGGTCAGCA AGAGAGAAGG AGAGCTAGAG 
AGCAACACGA AAGAGGAAGA GGACCTTCGC AAGAGGAATT GGGACAGAAA CAGCGAAGCA 
GGGAAGGAGG ATGGTTTGAC TGATGCACAG CAACAGTTTT CAGTGAAAGA AACAAACTTT 
TCAGAGGGAA ACTTAAAATT GAAAATTGGC CTTCAGGCTA AGAGAACTAA AAAACCTCCA 
AAAAACTTGG AGAACTATGT ATGTCGACCT GCCATAAAAA CAACTATCAA GCACCCAAGG 
AAAGCACTTA AAGGTGGGAA GATGACAGAG GAGAAGGATG AGCACTGTCC TTCAAGACGG 
GACCCTTCAA AGTTGTACAA GAAAACAGGT GATGTTGCAG CCGTTGAATG CCAATCTGAA 
GAGAGCATCC ATCTTCATTC ACAGGGAGAA AACAGTCCTT TGTCTAAGAA GCTTTCTCCA 
GGACACTCAG AGATGACAGA TTACATGAAT GCAGCATCAT CTACTCTTGT TGGTAGCCGG 
GATCCTGATT TAAAGGATAG AGCATTACTT AATGGAGGAA CAAGTGTAAC AGAAAAGTTG 
GCACAGCTTA TTGCAACCTG TCCTCCCTCT AAGTCTTCCA AGACTAAACC GAAGAAGTTA 
GGAAGTGGCA CCAGCGCAGG ATTGGTTAGT AAGGATTTGA TCAGGAAAGC AGGTGTTGGC 
TCTGTAGCTG GAATAATACA TAAAGATTTA ATTAAAAAAC CAGCTATCAG CACAGCAGTT 
GGATTGGTAA CTAAAGATCC TGGGAAAAAG CCAGTGTTTA ATGCAACAGT AGGATTGGTC 
AATAAGGACT CTGTGAAAAA ACTAGGAGCT GGCACTACAG CGGTATTCAT TAATAAAGAC 
TTAGGCAGAA AGCCAGGGAC TGTCACTACA GTAGGACTGC TGAGCAAGGA TTCAGGAAAG 
AAGCTAGGAA TTGGTATTGT TCCAGGTTTA GTGAATAAGG AATCTGGAAA GAAGTTAGGA 
CTCGGCACTG TGGTCGGACT GGTTAATAAA GACTTGGGCA AGAAGTTGAG TTCTACTGTC 
GGTCTAGTGG CCAAGGACTG TGCAAAGAAG ATTGTAGCCA ATTCAACAAT GGGATTAGTT 
ACTAAAGACA TGGGAAAGAA ACTAGTGAGT TGTTCTATGG CAGGATTGGT CAATAAAGAT 
GCCATAAACC TTAAAGCCGA AGCACTGCTC CCCACTCAGG AACCACTTAA GGCTTCTTGT 
AGTACAAACA TCAGTAGTCA TGAAAATCAG GAACTTTCTG AATCCCTGAA AGATAGTACC 
ACCAGCAAAA CTTTTGAGAA GAATGTTATA CGGCAGAGTA AAGAAAGCAT ATTGGAAAAG 
TTCTCAGTTC GAAAAGAAAT CATTAATTTG GAGAAAGAAA TGTTTAACGA GGGAACATGC 
ATTCAGCAAG ACAGTTTCTC ATCCAATGAC AGGGGGCCTT ATGAAACCTC AAAGCATGAA 
AAGCAACCTC CCGTGTATTG CACTTCTCCA GACTTTCAAA TGGGAGCTGC TTCTGATGCA 
TCTATGGCAA AATCCCCATT TAGTGCAGTA GGAGAAAGCA ATCTCCCTTC CCCATCACCT 
ACTGTATCTG TTAATCCTTT AACCAGAAGT CCCCCTGAAA CTTCTTCACA GTTGGCTCCT 
AATCCGTTAC TTTTAAGTCC TACCACAGAA CTAATGGAAG ACATTTCTGA ATCCGTTGGG 
AAGAACCAGT TTACTTCTGA AAGTACCCAC TTGAACATTG GTCATAGGTC TGTGGGTCAC 
AGCATGAATA TTGAATGTAA AGGGATTGAT AAAGAGCTAA CTGATTCAAA AACTGCACAT 
ATAGATATTC CAAGAATAAG CTCTTCCCTG GGAAAAAAGC CAAGTTTGAC TTCTGAATCC 
AGTATTCACA CAATTACCCC TTCAGTTGTT AACTTCACTA GTTTATTTAG TAACAAGCCG 
TTTCTAAAAC TTGGTGCAGT ATCTGCTTCT GACAAACACT GCCAGGTTGC TGAAAGCCTA 
AGCTCTAGTT TGCAGTCCAA ACCATTAAAA AAAAGGAAAG GAAGAAAAGC TCGGTGGACT 
AAAGTGGTGG CAAGAAGCAC ATGCCGGTCT CCAAAAGGGC TAGATTTAGA AAGATCAGAG 
CTTTTTAAAA GTGTTTCATG TAGCTCACTA TCAAATAGTA ATTCTGAGCC AGCCAAGTTC 
ATGAAAAACA TTGGACCATC TTCATTTGTA GATCATGACT TCCTTAAACG CCGATTGCCA 
AAGTTGAGCA AGTCCACAAC TCCATCTCTT GCTCTCTTAA CTGATAGTGA AAAACCATCT 
CACAAGGCTT TTGCTACTCA CAAATTATCC TCCAGTATGT GTGTCTCTAG TGACCTTTTG 
TCTGATATTT ATAAGCCCAA AAGAGGAAGA CCTAAAGCTA AAGAGATGCC TCAACTGGAA 
GGTCCACCTA AAAGGACTTT AAAAATCCCT GCCTCTAAAG TTTTTTCTTT GCAGTCTAAG 
GAAGAACAAG AACCCCCAAT TTTACAGCCA GAAATTGAAA TTCCTTCCTT CAAACAAAGT 
CTTTCTGTAT CTCCTTTTCC AAAAAAGCGA GGCAGACCTA AGAGGCAGTT GAGGTCACCG 
GTCAAGATGA AGCCACCTGT ACTATCAGTG GCTCCATTTG TTGCCACGGA GAGTCCAAGC 
AAGCTGGAGT CTGAAAGTGA TAACCATCGA AGCAGCAGTG ATTTCTTTGA GAGTGAGGAT 
CAGCTTCAGG ACCCAGATGA TCTGGACGAC AGTCATAGGC CGGCTGTCTG CAGCATGAGT 
GACCTTGAGA TGGAACCAGA TAAAAAAATT ACAAAGAGAA ACAATGGACA ATTAATGAAA 
ACAATTATCC GCAAAATAAA TAAAATGAAG ACTTTAAAAA GGAAGAAACT GTTGAATCAG 
ATTCTTTCAA GCTCTGTAGA ATCAAGTAAT AAAGGGAAAG TGCAATCCAA ACTCCATAAT 
ACAGTGTCAA GTCTTGCTGC CACGTTTGGC TCTAAATTGG GCCAACAGAT AAATGTCAGC 
AAGAAAGGAA CCATTTACAT AGGAAAGAGA AGGGGTCGAA AACCAAAAAC TGTCTTAAAT 
GGCATTCTTT CTGGTAGCCC TACCAGCCTT GCTGTCCTTG AGCAAACTGC TCAACAGGCT 
GCCGGCTCAG CATTGGGACA GATACTTCCC CCTTTACTGC CTTCATCTGC TAGTAGCTCA 
GAGATTCTCC CATCGCCTGT TTGCTCTCAG TCTTCTGGGA CTAGTGGTGG TCAGAGCCCT 
GTAAGTAGTG ATGCAGGCTT TGTTGAACCC AGCTCAGTGC CATATTTGCA TTTACACTCC 
AGACAGGGCA GTATGATTCA GACTCTTGCG ATGAAAAAGG CCTCAAAGGG GAGGAGGCGA 
CTTTCTCCTC CTACTTTGTT GCCAAATTCT CCTTCACACT TGAGTGAACT CACCTCTCTG 
AAAGAAGCTA CTCCTTCTCC CATTAGTGAG TCTCATAGTG ATGAGACCAT TCCCAGTGAT 
AGTGGAATTG GAACAGATAA TAACAGCACA TCGGACAGAG CAGAGAAATT TTGTGGACAG 
AAAAAGAGGA GGCACTCTTT TGAACATGTT TCTCTGATTC CCCCTGAAAC CTCTACAGTC 
CTGAGCAGTC TTAAAGAAAA ACATAAACAT AAATGTAAGC GCCGGAATCA CGATTACCTC 
AGCTATGACA AGATGAAAAG GCAGAAACGA AAACGGAAAA AGAAATACCC CCAGCTCCGT 
AGCAGACAGG ATCCAGACTT TATTGCGGAT CTGGAGGAAT TGATAAGTCG GCTGAGTGAA 
ATTCGAATCA CCCATCGAAG CCATCATTTT ATCCCCCGAG ACCTGCTGCC AACTATTTTT 
CGAATCAACT TTAATAGTTT CTATACACAT CCTTCTTTCC CCTTAGACCC CTTGCACTAC 
ATTCGAAAAC CTGACTTAAA AAAGAAGAGA GGGAGACCTC CTAAGATGAG GGAGGCAATG 
GCTGAGATGC CCTTCATGCA CAGCCTTAGT TTTCCTCTTT CTAGTACTGG ATTCTATCCC 
TCCTATGGCA TGCCTTACTC TCCCTCACCC CTTACAGCCA CTCCCCTAGG ATTAGGTTAC 
TATGGAAGGT ACCCTCCCAC TCTCTATCCT CCTCCTCCTT CACCTTCTTT CACCCCCCCA 
CTTCCTCCTC CTTCCTATAT GCATGCTGGC CATTTACTTC TCAATCCAAC CAAATACCAT 
AAGAAAAAGC ATAAGCTACT TCGCCAGGAG GCCTTTCTAA CAACCAGCAG GACTCCCCTC 
CTTTCCATGA GTACCTACCC TAGTGTTCCT CCTGAGATGG CCTATGGTTG GATGGTTGAG 
CACAAACATA GGCATCGTCA CAAGCACAGA GAACACCGTT CTTCTGAGCA ACCCCAGGTT 
TCCATGGATG CTGGCTCTTC CAGATCTGTT TTGGAGTCTT TGAAGCGCTA TCGATTTGGA 
AAGGAAACTG TTGGAGAGCG ATATAAACAT AAGGAAAAGC ACCGATGTCA TATGTCCTGC 
CCTCATCTCT CTCCTTCGAA AAGCTTAATA AATAGAGAAG AACAATGGGT CCACCGAGAG 
CCTTCAGAAT CTAGTCCATT GGCTTTGGGA TTGCAAACAC CTTTACAGAT TGACTGTTCC 
GAAAGTTCTC CAAGTTTATC TCTTGGAGGA TTCACTCCCA ACTCTGATCC GGCCAGCAGT 
GATGAACATA CAAACCTTTT CACAAGTGCA ATAGTGCAAG GCAGCTGCAG AGTTTCAAAC 
CCTAACTCCA GTGGCCGGAA GAAATTAACT GATAGCCCTG GACTGTTTTC TGCACAGGAC 
ACTTCACTAA TTCGGCCTCA CAGAAAGGAG CCACTGCCTT CCAGCGAAAG GGCAGTACAG 
ACCATGACAG GTTCCCAGCC AACCTCAGAT AAGTCTTCCC AGCGACCATC AGAGAGCACA 
AACTGTAGCC CTACCCGGAA GAGGTCTTCA TCTGAGAGTA CTTCTTCAAC AGTAAACGGA 
CTTCCTTCTC GAAGTCCAAG ATTGCTTGCC CCTGGGGATG ACTCTGTGGA TAGTCTGCTG 
CAGCGGATGG TACAACATGA GGACCAAGAG CCCCTGGAGA AGAATCTAGA TGCCGTGATT 
GCATCTGCCT CGGGGCCACC TTCTTCCAGT CCAGGCCACA GCCACAGCAA GGAACGAGCC 
CTGGGAAAAC CAGATAGCCT TCTAGTGCCC GCAGCCCCCA GTGACTCTTG TAGCAGCAGC 
ATCTCACTCC TGTCTGAGAA GTTGCCGAGC AGCTGCTCAC CCCACCATAT CAAGAGGAGT 
GTCGTGGAAG CCATGCAGCG CCAAGCTCGG AAAATGTGCA ATTATGACAA AATCTTGGCC 
ACAAAGAAAA ACCTGGACCA TGTCAATAAA ATATTAAAAG CCAAAAAGCT TCAAAGACAG 
GCCAGGACAG GGAATAACTT CGTGAAACGC AGACCAGGGC GACCTCGGAA ATGCCCCCTC 
CAGGCAGTGG TGTCAATGCA AGCATTCCAG GCTGCCCGAT TCGTCAGCCC CGATGCTAAT 
GAAGGTGAAG AAAGAGCAGC CCTGCCCCTC CGTCCAGACA CAGTTACGGA TGTCATCGAG 
GCTGTTGTGC AGAGCGTAAA CCTGAGTCCA GAACATAAGA AGGGGCTGAA GAGGAAAAGT 
TGGCTGTTGG AAGAACAGAC CAAGAAAAAG CAGAAGCCAT TCCCAGAGGA AGAACAAGAG 
CATCCCAAGA GCTTTACTGA AGCAGCAGTT GAGATTCCCA GTCCTCCTGA AACCCCAGCC 
AAGCCTTCCG AACCTGAAAG TACCTTGCAG CCTGTGCTTT CTCTCATCCC AAGGGAAAAG 
AAGGCCCCAC GTCCCCCAAA GAAGAAGTAT CAGAAAGCAG GGCTGTATTC TGACGTTTAC 
AAAACTACAG ACCCAAAGAG CCGATTGATC CAATTAAAGA AAGAAAAGTT GGAATACACT 
CCAGGAGAGC ATGAGTATGG ATTATTCCCA GCCCCTATTC ATGTTGGAAA GTATCTAAGG 
CAAAAGAGAA TTGACTTCCA GCTTCCCTAT GACATCCTTT GGCAGTGGAA ACATAATCAG 
CTATACAAAA AGCCAGATGT CCCACTATAT AAAAAAATCC GTTCAAACGT CTACGTGGAT 
GTCAAACCCC TTTCTGGTTA CGAGGCCACC ACCTGTAACT GTAAGAAGCC AGAGGATGAC 
ACCGGGAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCT 
AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGAGGCA TGAGTGGGTG 
CAGTGTCTAG AACGATTTCG AGCTGAGGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC 
CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGGGAGG TTGTCAGCGA ACAGGAGTTC 
AGGAACAGAA TGATTGAGCA GTATCATAAT CACAGTGACC ACTATTGCCT GAACCTGGAT 
AGTGGGATGG TGATTGACAG TTATCGGATG GGTAATGAGG CCCGATTCAT CAACCACAGC 
TGTGACCCAA ACTGTGAAAT GCAGAAATGG TCTGTCAATG GAGTATACCG GATTGGACTG 
TATGCTCTTA AAGATATGCC AGCTGGTACC GAGCTCACTT ATGATTACAA TTTCCATTCC 
TTTAATGTTG AGAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT 
GGGGGCAAGA GTCAGCGCGT GAACGGACTC GCCAGCAGCA AAAGCAGCCA GCCTGTGGCT 
ACCCATAAAA AGTCTGGACG GTCAAAAGAG AAGAGAAAGT CAAAGCACAA GCTGAAGAAA 
AGGAGAGGCC ACCTCTCCGA AGAACCCAGT GAAAACATCA ACACTCCAAC AAGATTGACC 
CCCCAATTAC AAATGAAGCC AATGTCTAAT CGAGAGAGGA ACTTCGTGTT AAAGCATCAT 
GTATTCTTGG TCCGAAACTG GGAGAAGATC CGACAGAAAC AAGAGGAAGT AAAGCATACC 
AGCGATAATC TCCACTCTGC ATCATTATAC ACCCGCTGGA ATGGCATCTG CCGAGATGAT 
GGGAACATCA AGTCTGACGT CTTCATGACC CAGTTCTCCG CCCTGCAGAC AGCACGATCA 
GTTCGAACAA GACGCCTGGC AGCAGCAGAG GAAAACATCG AGGTGGCACG GGCAGCCCGC 
CTCGCCCAAA TCTTCAAAGA GATCTGTGAT GGCATCATCT CCTACAAAGA TTCTTCCCGA 
CAAGCTTTGG CAGCACCCCT CTTGAACCTC CCCCCAAAGA AAAAGAATGC TGATTACTAT 
GAGAAGATCT CAGAGCCCCT GGACTTGGCC ACCATAGAGA GGCAGATCCT CACCGGCCAT 
TACAAGACCG TGGAGGCCTT TGACGCTGAC ATGCTCAAGG TGTTTCGGAA TGCTGAGAAG 
TACTACGGGC GTAAATCCCC AATTGGGAGA GATGTTTGCC GTCTACGGAA GACCTACTAC 
AACGCCAGGC ATGAGGCATC AGCCCAGATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG 
GCCGACAGCA GCGAAACATC TGTCTCTGAG AAGGAGAACG TGCATGAGAA GGATGACGAT 
GTGATCCGCT GTGTCTGTGG CCTCCACAAG GATGAGGGCC TCATGATCCA GTGTGACAAG 
TGCATGGTAT GGCAGCACTG TGACTGTATG GGGGTGAACT CAGACGTGGA GCATTACCTG 
TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC 
TATGCCCAGC CCGGCTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GTTGCTTCGT 
CAAGGTGACT GTGTGTACCT GATGCGAGAT AGCCGGCGCA CCCCTGATGG CCACCCAGTC 
CGCCAGTCCT ACCGACTGTT ATCACACATT AACCGAGATA AACTTGACAT CTTCCGCATT 
GAGAAGCTCT GGAAGAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA CTACTTCCGC 
CCCCACGAGA CCCACCACTC TCCGTCACGC CGCTTCTATC ACAATGAGCT ATTTAGGGTA 
CCACTCTATG AGATCATTCC CTTGGAGGCT GTGGTGGGGA CCTGCTGTGT ACTAGACCTT 
TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTATA CATCTGTGAC 
TATCGGCTTG ACAAGTCCGC ACACCTGTTT TACAAGATTC ACCGGAATCG CTATCCTGTC 
TGCACCAAAC CCTACGCCTT TGACCACTTC CCCAAGAAGC TCACTCCTAA AAGAGATTTC 
TCACCTCATT ATGTCCCAGA CAACTACAAG AGGAACGGAG GGCGGTCATC CTGGAAGTCT 
GAGCGCCCAA AGCCACCTCT AAAAGACCTG GGCCAGGAGG ATGACGCTCT GCCCTTGATT 
GAAGAGGTTC TGGCCAGCCA GGAGCAAGCC ACCAATGAGC TGCCCAGCCT GGAGGAGCCA 
GAACGGGAAG TGGCCCCTGT GGAAGTCAGC GAGGGTGAAA AGAAAGCAGA GGAAGGTGGT 
CCAGAATCTC AGGCTGCCTG TACCCCTGAG GAGCGACGGC ACAGCCAACG GGAACGGCTC 
AACCAGATCC TACTTGGCCT CCTGGAAAAA ATCCCTGGGA AAAATGCCAT CGATGTGACC 
TACTTGCTGG AGGAAGGGTC AGGCAGGAAG CTTCGAAGGC GCACTTTGTT CATCCCAGAG 
AACAGCTTTC GGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970812.1
CDS	362..9259
Translation	MDPRNTAMLGLGSDSEGFSRKSPSALGTGTVVSKREGELESNTKEEEDLRKRNWDRNSEAGKEDGLTDAQQQFSVKETNFSEGNLKLKIGLQAKRTKKPPKNLENYVCRPAIKTTIKHPRKALKGGKMTEEKDEHCPSRRDPSKLYKKTGDVAAVECQSEESIHLHSQGENSPLSKKLSPGHSEMTDYMNAASSTLVGSRDPDLKDRALLNGGTSVTEKLAQLIATCPPSKSSKTKPKKLGSGTSAGLVSKDLIRKAGVGSVAGIIHKDLIKKPAISTAVGLVTKDPGKKPVFNATVGLVNKDSVKKLGAGTTAVFINKDLGRKPGTVTTVGLLSKDSGKKLGIGIVPGLVNKESGKKLGLGTVVGLVNKDLGKKLSSTVGLVAKDCAKKIVANSTMGLVTKDMGKKLVSCSMAGLVNKDAINLKAEALLPTQEPLKASCSTNISSHENQELSESLKDSTTSKTFEKNVIRQSKESILEKFSVRKEIINLEKEMFNEGTCIQQDSFSSNDRGPYETSKHEKQPPVYCTSPDFQMGAASDASMAKSPFSAVGESNLPSPSPTVSVNPLTRSPPETSSQLAPNPLLLSPTTELMEDISESVGKNQFTSESTHLNIGHRSVGHSMNIECKGIDKELTDSKTAHIDIPRISSSLGKKPSLTSESSIHTITPSVVNFTSLFSNKPFLKLGAVSASDKHCQVAESLSSSLQSKPLKKRKGRKARWTKVVARSTCRSPKGLDLERSELFKSVSCSSLSNSNSEPAKFMKNIGPSSFVDHDFLKRRLPKLSKSTTPSLALLTDSEKPSHKAFATHKLSSSMCVSSDLLSDIYKPKRGRPKAKEMPQLEGPPKRTLKIPASKVFSLQSKEEQEPPILQPEIEIPSFKQSLSVSPFPKKRGRPKRQLRSPVKMKPPVLSVAPFVATESPSKLESESDNHRSSSDFFESEDQLQDPDDLDDSHRPAVCSMSDLEMEPDKKITKRNNGQLMKTIIRKINKMKTLKRKKLLNQILSSSVESSNKGKVQSKLHNTVSSLAATFGSKLGQQINVSKKGTIYIGKRRGRKPKTVLNGILSGSPTSLAVLEQTAQQAAGSALGQILPPLLPSSASSSEILPSPVCSQSSGTSGGQSPVSSDAGFVEPSSVPYLHLHSRQGSMIQTLAMKKASKGRRRLSPPTLLPNSPSHLSELTSLKEATPSPISESHSDETIPSDSGIGTDNNSTSDRAEKFCGQKKRRHSFEHVSLIPPETSTVLSSLKEKHKHKCKRRNHDYLSYDKMKRQKRKRKKKYPQLRSRQDPDFIADLEELISRLSEIRITHRSHHFIPRDLLPTIFRINFNSFYTHPSFPLDPLHYIRKPDLKKKRGRPPKMREAMAEMPFMHSLSFPLSSTGFYPSYGMPYSPSPLTATPLGLGYYGRYPPTLYPPPPSPSFTPPLPPPSYMHAGHLLLNPTKYHKKKHKLLRQEAFLTTSRTPLLSMSTYPSVPPEMAYGWMVEHKHRHRHKHREHRSSEQPQVSMDAGSSRSVLESLKRYRFGKETVGERYKHKEKHRCHMSCPHLSPSKSLINREEQWVHREPSESSPLALGLQTPLQIDCSESSPSLSLGGFTPNSDPASSDEHTNLFTSAIVQGSCRVSNPNSSGRKKLTDSPGLFSAQDTSLIRPHRKEPLPSSERAVQTMTGSQPTSDKSSQRPSESTNCSPTRKRSSSESTSSTVNGLPSRSPRLLAPGDDSVDSLLQRMVQHEDQEPLEKNLDAVIASASGPPSSSPGHSHSKERALGKPDSLLVPAAPSDSCSSSISLLSEKLPSSCSPHHIKRSVVEAMQRQARKMCNYDKILATKKNLDHVNKILKAKKLQRQARTGNNFVKRRPGRPRKCPLQAVVSMQAFQAARFVSPDANEGEERAALPLRPDTVTDVIEAVVQSVNLSPEHKKGLKRKSWLLEEQTKKKQKPFPEEEQEHPKSFTEAAVEIPSPPETPAKPSEPESTLQPVLSLIPREKKAPRPPKKKYQKAGLYSDVYKTTDPKSRLIQLKKEKLEYTPGEHEYGLFPAPIHVGKYLRQKRIDFQLPYDILWQWKHNQLYKKPDVPLYKKIRSNVYVDVKPLSGYEATTCNCKKPEDDTGKGCVDDCLNRMIFAECSPNTCPCGEQCCNQRIQRHEWVQCLERFRAEEKGWGIRTKEPLKAGQFIIEYLGEVVSEQEFRNRMIEQYHNHSDHYCLNLDSGMVIDSYRMGNEARFINHSCDPNCEMQKWSVNGVYRIGLYALKDMPAGTELTYDYNFHSFNVEKQQLCKCGFEKCRGIIGGKSQRVNGLASSKSSQPVATHKKSGRSKEKRKSKHKLKKRRGHLSEEPSENINTPTRLTPQLQMKPMSNRERNFVLKHHVFLVRNWEKIRQKQEEVKHTSDNLHSASLYTRWNGICRDDGNIKSDVFMTQFSALQTARSVRTRRLAAAEENIEVARAARLAQIFKEICDGIISYKDSSRQALAAPLLNLPPKKKNADYYEKISEPLDLATIERQILTGHYKTVEAFDADMLKVFRNAEKYYGRKSPIGRDVCRLRKTYYNARHEASAQIDEIVGETASEADSSETSVSEKENVHEKDDDVIRCVCGLHKDEGLMIQCDKCMVWQHCDCMGVNSDVEHYLCEQCDPRPVDREVPMIPRPHYAQPGCVYFICLLRDDLLLRQGDCVYLMRDSRRTPDGHPVRQSYRLLSHINRDKLDIFRIEKLWKNEKEERFAFGHHYFRPHETHHSPSRRFYHNELFRVPLYEIIPLEAVVGTCCVLDLYTYCKGRPKGVKEQDVYICDYRLDKSAHLFYKIHRNRYPVCTKPYAFDHFPKKLTPKRDFSPHYVPDNYKRNGGRSSWKSERPKPPLKDLGQEDDALPLIEEVLASQEQATNELPSLEEPEREVAPVEVSEGEKKAEEGGPESQAACTPEERRHSQRERLNQILLGLLEKIPGKNAIDVTYLLEEGSGRKLRRRTLFIPENSFRK

Target ORF information:

RefSeq Version	XM_014115337.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115337.2

ORF Insert Sequence:

ATGGACCCTA GAAATACTGC TATGTTAGGA TTGGGTTCTG ACTCGGAAGG ATTTTCAAGG 
AAGAGTCCCT CTGCCCTTGG TACCGGCACA GTGGTCAGCA AGAGAGAAGG AGAGCTAGAG 
AGCAACACGA AAGAGGAAGA GGACCTTCGC AAGAGGAATT GGGACAGAAA CAGCGAAGCA 
GGGAAGGAGG ATGGTTTGAC TGATGCACAG CAACAGTTTT CAGTGAAAGA AACAAACTTT 
TCAGAGGGAA ACTTAAAATT GAAAATTGGC CTTCAGGCTA AGAGAACTAA AAAACCTCCA 
AAAAACTTGG AGAACTATGT ATGTCGACCT GCCATAAAAA CAACTATCAA GCACCCAAGG 
AAAGCACTTA AAGGTGGGAA GATGACAGAG GAGAAGGATG AGCACTGTCC TTCAAGACGG 
GACCCTTCAA AGTTGTACAA GAAAACAGGT GATGTTGCAG CCGTTGAATG CCAATCTGAA 
GAGAGCATCC ATCTTCATTC ACAGGGAGAA AACAGTCCTT TGTCTAAGAA GCTTTCTCCA 
GGACACTCAG AGATGACAGA TTACATGAAT GCAGCATCAT CTACTCTTGT TGGTAGCCGG 
GATCCTGATT TAAAGGATAG AGCATTACTT AATGGAGGAA CAAGTGTAAC AGAAAAGTTG 
GCACAGCTTA TTGCAACCTG TCCTCCCTCT AAGTCTTCCA AGACTAAACC GAAGAAGTTA 
GGAAGTGGCA CCAGCGCAGG ATTGGTTAGT AAGGATTTGA TCAGGAAAGC AGGTGTTGGC 
TCTGTAGCTG GAATAATACA TAAAGATTTA ATTAAAAAAC CAGCTATCAG CACAGCAGTT 
GGATTGGTAA CTAAAGATCC TGGGAAAAAG CCAGTGTTTA ATGCAACAGT AGGATTGGTC 
AATAAGGACT CTGTGAAAAA ACTAGGAGCT GGCACTACAG CGGTATTCAT TAATAAAGAC 
TTAGGCAGAA AGCCAGGGAC TGTCACTACA GTAGGACTGC TGAGCAAGGA TTCAGGAAAG 
AAGCTAGGAA TTGGTATTGT TCCAGGTTTA GTGAATAAGG AATCTGGAAA GAAGTTAGGA 
CTCGGCACTG TGGTCGGACT GGTTAATAAA GACTTGGGCA AGAAGTTGAG TTCTACTGTC 
GGTCTAGTGG CCAAGGACTG TGCAAAGAAG ATTGTAGCCA ATTCAACAAT GGGATTAGTT 
ACTAAAGACA TGGGAAAGAA ACTAGTGAGT TGTTCTATGG CAGGATTGGT CAATAAAGAT 
GCCATAAACC TTAAAGCCGA AGCACTGCTC CCCACTCAGG AACCACTTAA GGCTTCTTGT 
AGTACAAACA TCAGTAGTCA TGAAAATCAG GAACTTTCTG AATCCCTGAA AGATAGTACC 
ACCAGCAAAA CTTTTGAGAA GAATGTTATA CGGCAGAGTA AAGAAAGCAT ATTGGAAAAG 
TTCTCAGTTC GAAAAGAAAT CATTAATTTG GAGAAAGAAA TGTTTAACGA GGGAACATGC 
ATTCAGCAAG ACAGTTTCTC ATCCAATGAC AGGGGGCCTT ATGAAACCTC AAAGCATGAA 
AAGCAACCTC CCGTGTATTG CACTTCTCCA GACTTTCAAA TGGGAGCTGC TTCTGATGCA 
TCTATGGCAA AATCCCCATT TAGTGCAGTA GGAGAAAGCA ATCTCCCTTC CCCATCACCT 
ACTGTATCTG TTAATCCTTT AACCAGAAGT CCCCCTGAAA CTTCTTCACA GTTGGCTCCT 
AATCCGTTAC TTTTAAGTCC TACCACAGAA CTAATGGAAG ACATTTCTGA ATCCGTTGGG 
AAGAACCAGT TTACTTCTGA AAGTACCCAC TTGAACATTG GTCATAGGTC TGTGGGTCAC 
AGCATGAATA TTGAATGTAA AGGGATTGAT AAAGAGCTAA CTGATTCAAA AACTGCACAT 
ATAGATATTC CAAGAATAAG CTCTTCCCTG GGAAAAAAGC CAAGTTTGAC TTCTGAATCC 
AGTATTCACA CAATTACCCC TTCAGTTGTT AACTTCACTA GTTTATTTAG TAACAAGCCG 
TTTCTAAAAC TTGGTGCAGT ATCTGCTTCT GACAAACACT GCCAGGTTGC TGAAAGCCTA 
AGCTCTAGTT TGCAGTCCAA ACCATTAAAA AAAAGGAAAG GAAGAAAAGC TCGGTGGACT 
AAAGTGGTGG CAAGAAGCAC ATGCCGGTCT CCAAAAGGGC TAGATTTAGA AAGATCAGAG 
CTTTTTAAAA GTGTTTCATG TAGCTCACTA TCAAATAGTA ATTCTGAGCC AGCCAAGTTC 
ATGAAAAACA TTGGACCATC TTCATTTGTA GATCATGACT TCCTTAAACG CCGATTGCCA 
AAGTTGAGCA AGTCCACAAC TCCATCTCTT GCTCTCTTAA CTGATAGTGA AAAACCATCT 
CACAAGGCTT TTGCTACTCA CAAATTATCC TCCAGTATGT GTGTCTCTAG TGACCTTTTG 
TCTGATATTT ATAAGCCCAA AAGAGGAAGA CCTAAAGCTA AAGAGATGCC TCAACTGGAA 
GGTCCACCTA AAAGGACTTT AAAAATCCCT GCCTCTAAAG TTTTTTCTTT GCAGTCTAAG 
GAAGAACAAG AACCCCCAAT TTTACAGCCA GAAATTGAAA TTCCTTCCTT CAAACAAAGT 
CTTTCTGTAT CTCCTTTTCC AAAAAAGCGA GGCAGACCTA AGAGGCAGTT GAGGTCACCG 
GTCAAGATGA AGCCACCTGT ACTATCAGTG GCTCCATTTG TTGCCACGGA GAGTCCAAGC 
AAGCTGGAGT CTGAAAGTGA TAACCATCGA AGCAGCAGTG ATTTCTTTGA GAGTGAGGAT 
CAGCTTCAGG ACCCAGATGA TCTGGACGAC AGTCATAGGC CGGCTGTCTG CAGCATGAGT 
GACCTTGAGA TGGAACCAGA TAAAAAAATT ACAAAGAGAA ACAATGGACA ATTAATGAAA 
ACAATTATCC GCAAAATAAA TAAAATGAAG ACTTTAAAAA GGAAGAAACT GTTGAATCAG 
ATTCTTTCAA GCTCTGTAGA ATCAAGTAAT AAAGGGAAAG TGCAATCCAA ACTCCATAAT 
ACAGTGTCAA GTCTTGCTGC CACGTTTGGC TCTAAATTGG GCCAACAGAT AAATGTCAGC 
AAGAAAGGAA CCATTTACAT AGGAAAGAGA AGGGGTCGAA AACCAAAAAC TGTCTTAAAT 
GGCATTCTTT CTGGTAGCCC TACCAGCCTT GCTGTCCTTG AGCAAACTGC TCAACAGGCT 
GCCGGCTCAG CATTGGGACA GATACTTCCC CCTTTACTGC CTTCATCTGC TAGTAGCTCA 
GAGATTCTCC CATCGCCTGT TTGCTCTCAG TCTTCTGGGA CTAGTGGTGG TCAGAGCCCT 
GTAAGTAGTG ATGCAGGCTT TGTTGAACCC AGCTCAGTGC CATATTTGCA TTTACACTCC 
AGACAGGGCA GTATGATTCA GACTCTTGCG ATGAAAAAGG CCTCAAAGGG GAGGAGGCGA 
CTTTCTCCTC CTACTTTGTT GCCAAATTCT CCTTCACACT TGAGTGAACT CACCTCTCTG 
AAAGAAGCTA CTCCTTCTCC CATTAGTGAG TCTCATAGTG ATGAGACCAT TCCCAGTGAT 
AGTGGAATTG GAACAGATAA TAACAGCACA TCGGACAGAG CAGAGAAATT TTGTGGACAG 
AAAAAGAGGA GGCACTCTTT TGAACATGTT TCTCTGATTC CCCCTGAAAC CTCTACAGTC 
CTGAGCAGTC TTAAAGAAAA ACATAAACAT AAATGTAAGC GCCGGAATCA CGATTACCTC 
AGCTATGACA AGATGAAAAG GCAGAAACGA AAACGGAAAA AGAAATACCC CCAGCTCCGT 
AGCAGACAGG ATCCAGACTT TATTGCGGAT CTGGAGGAAT TGATAAGTCG GCTGAGTGAA 
ATTCGAATCA CCCATCGAAG CCATCATTTT ATCCCCCGAG ACCTGCTGCC AACTATTTTT 
CGAATCAACT TTAATAGTTT CTATACACAT CCTTCTTTCC CCTTAGACCC CTTGCACTAC 
ATTCGAAAAC CTGACTTAAA AAAGAAGAGA GGGAGACCTC CTAAGATGAG GGAGGCAATG 
GCTGAGATGC CCTTCATGCA CAGCCTTAGT TTTCCTCTTT CTAGTACTGG ATTCTATCCC 
TCCTATGGCA TGCCTTACTC TCCCTCACCC CTTACAGCCA CTCCCCTAGG ATTAGGTTAC 
TATGGAAGGT ACCCTCCCAC TCTCTATCCT CCTCCTCCTT CACCTTCTTT CACCCCCCCA 
CTTCCTCCTC CTTCCTATAT GCATGCTGGC CATTTACTTC TCAATCCAAC CAAATACCAT 
AAGAAAAAGC ATAAGCTACT TCGCCAGGAG GCCTTTCTAA CAACCAGCAG GACTCCCCTC 
CTTTCCATGA GTACCTACCC TAGTGTTCCT CCTGAGATGG CCTATGGTTG GATGGTTGAG 
CACAAACATA GGCATCGTCA CAAGCACAGA GAACACCGTT CTTCTGAGCA ACCCCAGGTT 
TCCATGGATG CTGGCTCTTC CAGATCTGTT TTGGAGTCTT TGAAGCGCTA TCGATTTGGA 
AAGGAAACTG TTGGAGAGCG ATATAAACAT AAGGAAAAGC ACCGATGTCA TATGTCCTGC 
CCTCATCTCT CTCCTTCGAA AAGCTTAATA AATAGAGAAG AACAATGGGT CCACCGAGAG 
CCTTCAGAAT CTAGTCCATT GGCTTTGGGA TTGCAAACAC CTTTACAGAT TGACTGTTCC 
GAAAGTTCTC CAAGTTTATC TCTTGGAGGA TTCACTCCCA ACTCTGATCC GGCCAGCAGT 
GATGAACATA CAAACCTTTT CACAAGTGCA ATAGTGCAAG GCAGCTGCAG AGTTTCAAAC 
CCTAACTCCA GTGGCCGGAA GAAATTAACT GATAGCCCTG GACTGTTTTC TGCACAGGAC 
ACTTCACTAA TTCGGCCTCA CAGAAAGGAG CCACTGCCTT CCAGCGAAAG GGCAGTACAG 
ACCATGACAG GTTCCCAGCC AACCTCAGAT AAGTCTTCCC AGCGACCATC AGAGAGCACA 
AACTGTAGCC CTACCCGGAA GAGGTCTTCA TCTGAGAGTA CTTCTTCAAC AGTAAACGGA 
CTTCCTTCTC GAAGTCCAAG ATTGCTTGCC CCTGGGGATG ACTCTGTGGA TAGTCTGCTG 
CAGCGGATGG TACAACATGA GGACCAAGAG CCCCTGGAGA AGAATCTAGA TGCCGTGATT 
GCATCTGCCT CGGGGCCACC TTCTTCCAGT CCAGGCCACA GCCACAGCAA GGAACGAGCC 
CTGGGAAAAC CAGATAGCCT TCTAGTGCCC GCAGCCCCCA GTGACTCTTG TAGCAGCAGC 
ATCTCACTCC TGTCTGAGAA GTTGCCGAGC AGCTGCTCAC CCCACCATAT CAAGAGGAGT 
GTCGTGGAAG CCATGCAGCG CCAAGCTCGG AAAATGTGCA ATTATGACAA AATCTTGGCC 
ACAAAGAAAA ACCTGGACCA TGTCAATAAA ATATTAAAAG CCAAAAAGCT TCAAAGACAG 
GCCAGGACAG GGAATAACTT CGTGAAACGC AGACCAGGGC GACCTCGGAA ATGCCCCCTC 
CAGGCAGTGG TGTCAATGCA AGCATTCCAG GCTGCCCGAT TCGTCAGCCC CGATGCTAAT 
GAAGGTGAAG AAAGAGCAGC CCTGCCCCTC CGTCCAGACA CAGTTACGGA TGTCATCGAG 
GCTGTTGTGC AGAGCGTAAA CCTGAGTCCA GAACATAAGA AGGGGCTGAA GAGGAAAAGT 
TGGCTGTTGG AAGAACAGAC CAAGAAAAAG CAGAAGCCAT TCCCAGAGGA AGAACAAGAG 
CATCCCAAGA GCTTTACTGA AGCAGCAGTT GAGATTCCCA GTCCTCCTGA AACCCCAGCC 
AAGCCTTCCG AACCTGAAAG TACCTTGCAG CCTGTGCTTT CTCTCATCCC AAGGGAAAAG 
AAGGCCCCAC GTCCCCCAAA GAAGAAGTAT CAGAAAGCAG GGCTGTATTC TGACGTTTAC 
AAAACTACAG ACCCAAAGAG CCGATTGATC CAATTAAAGA AAGAAAAGTT GGAATACACT 
CCAGGAGAGC ATGAGTATGG ATTATTCCCA GCCCCTATTC ATGTTGGAAA GTATCTAAGG 
CAAAAGAGAA TTGACTTCCA GCTTCCCTAT GACATCCTTT GGCAGTGGAA ACATAATCAG 
CTATACAAAA AGCCAGATGT CCCACTATAT AAAAAAATCC GTTCAAACGT CTACGTGGAT 
GTCAAACCCC TTTCTGGTTA CGAGGCCACC ACCTGTAACT GTAAGAAGCC AGAGGATGAC 
ACCGGGAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCT 
AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGAGGCA TGAGTGGGTG 
CAGTGTCTAG AACGATTTCG AGCTGAGGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC 
CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGGGAGG TTGTCAGCGA ACAGGAGTTC 
AGGAACAGAA TGATTGAGCA GTATCATAAT CACAGTGACC ACTATTGCCT GAACCTGGAT 
AGTGGGATGG TGATTGACAG TTATCGGATG GGTAATGAGG CCCGATTCAT CAACCACAGC 
TGTGACCCAA ACTGTGAAAT GCAGAAATGG TCTGTCAATG GAGTATACCG GATTGGACTG 
TATGCTCTTA AAGATATGCC AGCTGGTACC GAGCTCACTT ATGATTACAA TTTCCATTCC 
TTTAATGTTG AGAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT 
GGGGGCAAGA GTCAGCGCGT GAACGGACTC GCCAGCAGCA AAAGCAGCCA GCCTGTGGCT 
ACCCATAAAA AGTCTGGACG GTCAAAAGAG AAGAGAAAGT CAAAGCACAA GCTGAAGAAA 
AGGAGAGGCC ACCTCTCCGA AGAACCCAGT GAAAACATCA ACACTCCAAC AAGATTGACC 
CCCCAATTAC AAATGAAGCC AATGTCTAAT CGAGAGAGGA ACTTCGTGTT AAAGCATCAT 
GTATTCTTGG TCCGAAACTG GGAGAAGATC CGACAGAAAC AAGAGGAAGT AAAGCATACC 
AGCGATAATC TCCACTCTGC ATCATTATAC ACCCGCTGGA ATGGCATCTG CCGAGATGAT 
GGGAACATCA AGTCTGACGT CTTCATGACC CAGTTCTCCG CCCTGCAGAC AGCACGATCA 
GTTCGAACAA GACGCCTGGC AGCAGCAGAG GAAAACATCG AGGTGGCACG GGCAGCCCGC 
CTCGCCCAAA TCTTCAAAGA GATCTGTGAT GGCATCATCT CCTACAAAGA TTCTTCCCGA 
CAAGCTTTGG CAGCACCCCT CTTGAACCTC CCCCCAAAGA AAAAGAATGC TGATTACTAT 
GAGAAGATCT CAGAGCCCCT GGACTTGGCC ACCATAGAGA GGCAGATCCT CACCGGCCAT 
TACAAGACCG TGGAGGCCTT TGACGCTGAC ATGCTCAAGG TGTTTCGGAA TGCTGAGAAG 
TACTACGGGC GTAAATCCCC AATTGGGAGA GATGTTTGCC GTCTACGGAA GACCTACTAC 
AACGCCAGGC ATGAGGCATC AGCCCAGATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG 
GCCGACAGCA GCGAAACATC TGTCTCTGAG AAGGAGAACG TGCATGAGAA GGATGACGAT 
GTGATCCGCT GTGTCTGTGG CCTCCACAAG GATGAGGGCC TCATGATCCA GTGTGACAAG 
TGCATGGTAT GGCAGCACTG TGACTGTATG GGGGTGAACT CAGACGTGGA GCATTACCTG 
TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC 
TATGCCCAGC CCGGCTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GTTGCTTCGT 
CAAGGTGACT GTGTGTACCT GATGCGAGAT AGCCGGCGCA CCCCTGATGG CCACCCAGTC 
CGCCAGTCCT ACCGACTGTT ATCACACATT AACCGAGATA AACTTGACAT CTTCCGCATT 
GAGAAGCTCT GGAAGAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA CTACTTCCGC 
CCCCACGAGA CCCACCACTC TCCGTCACGC CGCTTCTATC ACAATGAGCT ATTTAGGGTA 
CCACTCTATG AGATCATTCC CTTGGAGGCT GTGGTGGGGA CCTGCTGTGT ACTAGACCTT 
TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTATA CATCTGTGAC 
TATCGGCTTG ACAAGTCCGC ACACCTGTTT TACAAGATTC ACCGGAATCG CTATCCTGTC 
TGCACCAAAC CCTACGCCTT TGACCACTTC CCCAAGAAGC TCACTCCTAA AAGAGATTTC 
TCACCTCATT ATGTCCCAGA CAACTACAAG AGGAACGGAG GGCGGTCATC CTGGAAGTCT 
GAGCGCCCAA AGCCACCTCT AAAAGACCTG GGCCAGGAGG ATGACGCTCT GCCCTTGATT 
GAAGAGGTTC TGGCCAGCCA GGAGCAAGCC ACCAATGAGC TGCCCAGCCT GGAGGAGCCA 
GAACGGGAAG TGGCCCCTGT GGAAGTCAGC GAGGGTGAAA AGAAAGCAGA GGAAGGTGGT 
CCAGAATCTC AGGCTGCCTG TACCCCTGAG GAGCGACGGC ACAGCCAACG GGAACGGCTC 
AACCAGATCC TACTTGGCCT CCTGGAAAAA ATCCCTGGGA AAAATGCCAT CGATGTGACC 
TACTTGCTGG AGGAAGGGTC AGGCAGGAAG CTTCGAAGGC GCACTTTGTT CATCCCAGAG 
AACAGCTTTC GGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ASH1L cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

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