EDEM3 cDNA ORF clone, Canis lupus familiaris(Dog)

The following EDEM3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EDEM3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe09157 XM_005622430.3
Latest version!
Canis lupus familiaris ER degradation enhancing alpha-mannosidase like protein 3 (EDEM3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe09158 XM_003434974.4
Latest version!
Canis lupus familiaris ER degradation enhancing alpha-mannosidase like protein 3 (EDEM3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe09159 XM_005622431.3
Latest version!
Canis lupus familiaris ER degradation enhancing alpha-mannosidase like protein 3 (EDEM3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe09160 XM_537162.6
Latest version!
Canis lupus familiaris ER degradation enhancing alpha-mannosidase like protein 3 (EDEM3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe37426 XM_014115137.1
Latest version!
Canis lupus familiaris ER degradation enhancer, mannosidase alpha-like 3 (EDEM3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe37427 XM_014115138.1
Latest version!
Canis lupus familiaris ER degradation enhancer, mannosidase alpha-like 3 (EDEM3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe37428 XM_014115139.1
Latest version!
Canis lupus familiaris ER degradation enhancer, mannosidase alpha-like 3 (EDEM3), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe37429 XM_014115140.1
Latest version!
Canis lupus familiaris ER degradation enhancer, mannosidase alpha-like 3 (EDEM3), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
View Old Accession Versions >>
OCe09158 XM_003434974.3 Canis lupus familiaris ER degradation enhancer, mannosidase alpha-like 3 (EDEM3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe09159 XM_005622431.2 Canis lupus familiaris ER degradation enhancer, mannosidase alpha-like 3 (EDEM3), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe09157 XM_005622430.2 Canis lupus familiaris ER degradation enhancer, mannosidase alpha-like 3 (EDEM3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe09160 XM_537162.5 Canis lupus familiaris ER degradation enhancer, mannosidase alpha-like 3 (EDEM3), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe09157
    Clone ID Related Accession (Same CDS sequence) XM_005622430.3 , XM_005622430.2
    Accession Version XM_005622430.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2853bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005622430.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGCGAAG CCCGCGGCGG CCGGGGCTGT GGGTCCTCGG TCCCCCAGCG AGCGCCATGG 
    AGCCTGGTGG CGGCGGCGGC CGCGCTCTGC CTGGTGGCGG CCACGTCCGT GTGGACGGCG
    GGGGCCGCGC CCATGAGTAG GGAGGAGAGA CAGAAGCTTG GGAATCAAGT ACTGGAAATG
    TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCTGATGA ACTCATGCCT
    TTAACCTGTA GAGGTCGTGT TAGAGGCCAG GAGCCAAGTC GGGGTGATGT TGATGATGCC
    TTGGGAAAAT TTTCCCTGAC ACTGATTGAT TCTTTGGATA CTCTTGTGGT ATTAAATAAA
    ACTAAAGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGAG ATGTTAATTT AGATAATGAT
    GTAGTTGTAT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC
    TCGCTGGCGA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAACTT
    CTCCAAATGG CGAAGCAATT GGGTTACAAA CTTTTACCAG CTTTCAATAC TACCAGTGGC
    CTTCCTTATC CAAGAATTAA TTTAAAATTT GGTATCAGAA AACCGGAAGC TCGAACAGGA
    ACTGAAACAG ATACTTGTAC AGCTTGTGCA GGTACCTTGA TCCTTGAGTT CGCTGCTTTA
    AGTCGATTCA CAGGAGCAAC AATATTTGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC
    TGGGAAAAAA GACAGCGAAG TAGTAATTTA GTGGGTGTGA CTATAAATAT TCATACTGGA
    GACTGGGTAA GAAAAGATAG TGGAGTTGGA GCAGGGATTG ATTCATATTA TGAATATCTG
    TTGAAAGCTT ATGTCTTGCT TGGAGATGAC AGTTTCCTGG AAAGATTTAA TACACACTAT
    GATGCCATAA TGAGGTACAT TAGCCAGCCA CCTCTTCTGC TTGATGTGCA TATCCACAAG
    CCAATGCTGA ATGCTCGGAC TTGGATGGAT GCATTGCTCG CCTTTTTTCC AGGTTTGCAG
    GTCTTAAAGG GGGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA TCAGGTGATT
    AAAAAACATA ACTTCCTACC AGAGGCATTT ACTACAGATT TCAGGGTACA TTGGGCTCAA
    CATCCTTTAA GACCAGAATT TGCAGAAAGT ACCTACTTCT TATATAAAGC TACAGGAGAT
    CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TAAATAAATA TGCTAGAGTG
    CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGTACTGGAA GTCATGAGGA CAGAATGGAT
    TCTTTCTTCT TGGCTGAGAT GTTTAAATAT CTTTACCTAT TATTTGCGGA TAAAGAAGAC
    ATTATTTTTG ACATAGAAGA TTACATCTTT ACAACAGAAG CTCATCTGTT ACCTCTTTGG
    CTCTCTACTA CAAATCAAAG CATCTCTAAG AAGAATACAA CTTCAGAATA TACAGAACTG
    GATGACAGTA ATTTTGATTG GACTTGTCCA AACACTCAGA TTCTCTTCCC TAATGACCCA
    TTATATGCTC AGAGCATTCG TGAACCCTTG AAAAATGTGG TGGATAAGAG CTGTCCTAGA
    GGCATCATCA GAGTCAGAGA GGAGAGTTTC AGGAGTGGAG CTAAACCCCC TCTGAGAGCC
    AGAGATTTCA TGGCCACTAA CCCTGAACAT TTAGAAATCT TGAAGAAGAT GGGGGTGAGT
    TTGATTCACC TCAAAGATGG GAGAGTCCAG TTGGTTCAAC ATGCAATCCA AGCTGCTAGT
    TCAATTGATG CTGAGGATGG TTTGAGGTTC ATGCAGGAGA TGATCGAATT GTCAAGTCAG
    CAACAAAAAG AACAGCAGCT GCCTCCACGA GCTGTTCAAA TTGTATCCCA CCCATTTTTT
    GGCAGGGTAG TATTGACTGC TGGACCAGCT CAGTTTGGGC TGGATCTGTC TAAACATAAA
    GAGACAAGAG GATTTGTTGC AAGCAGTAAA CCATACAATG GTTGTTCAGA GCTTACTAAC
    CCCGAGGCAG TGATGGGAAA AATCGCTTTG ATACAAAGAG GACAGTGCAT GTTTGCAGAA
    AAGGCACGCA ACATTCAGAA TGCTGGTGCC ATTGGTGGCA TTGTTATTGA TGACAATGAG
    GGGAGCAGCA GTGATACTGC CCCTCTGTTC CAGATGGCAG GTGATGGAAA AGACACAGAC
    GACATCAAGA TCCCCATGCT ATTCTTATTC AGTAAAGAAG GAAGTATCAT ATTGGATGCC
    ATCCGGGAAT ATGAGGAGGT GGAAGTACTC CTTTCTGACA AAGCAAAAGA TAGAGCAGCA
    ATATTAAAAG GTAAAATGAT TCCAAGCTAC ATTATTAATA GTAATCCTGA AATGGAAAAT
    GAAGAACAAC CATCCTCTGA AAATGATTCT CAGAATCAGA GTGCTGAACA GATTACATCA
    AGTTCTCAGG AGGTTGATTT GGTGGATCAA GAGTCTCCTG AGGAAAGTTC TCTAAACTCA
    CAACCAGAAT CATCATCTCC AACAGATACA GACAATGCTG CAAGTGTTTC TGCTTCTGAA
    CAGAATTCTA ACCCCGTAGA AAACCATGAG ACTACAAGTC TTGATGGTGA ATGTACAGAT
    TTAGATAACC AACTTCAAGA ACAATCAGAA ACCGAAGAAG ATTCCAATCC TAATGTTAGC
    TGGGGTAAAA AGGTCCAGCC TATAGACTCC ATATTAGCAG ACTGGAATGA AGATATAGAA
    GCATTTGAAA TGATGGAGAA AGACGAACTA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005622487.1
    CDS221..3073
    Translation

    Target ORF information:

    RefSeq Version XM_005622430.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ER degradation enhancing alpha-mannosidase like protein 3 (EDEM3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005622430.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGCGAAG CCCGCGGCGG CCGGGGCTGT GGGTCCTCGG TCCCCCAGCG AGCGCCATGG 
    AGCCTGGTGG CGGCGGCGGC CGCGCTCTGC CTGGTGGCGG CCACGTCCGT GTGGACGGCG
    GGGGCCGCGC CCATGAGTAG GGAGGAGAGA CAGAAGCTTG GGAATCAAGT ACTGGAAATG
    TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCTGATGA ACTCATGCCT
    TTAACCTGTA GAGGTCGTGT TAGAGGCCAG GAGCCAAGTC GGGGTGATGT TGATGATGCC
    TTGGGAAAAT TTTCCCTGAC ACTGATTGAT TCTTTGGATA CTCTTGTGGT ATTAAATAAA
    ACTAAAGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGAG ATGTTAATTT AGATAATGAT
    GTAGTTGTAT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC
    TCGCTGGCGA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAACTT
    CTCCAAATGG CGAAGCAATT GGGTTACAAA CTTTTACCAG CTTTCAATAC TACCAGTGGC
    CTTCCTTATC CAAGAATTAA TTTAAAATTT GGTATCAGAA AACCGGAAGC TCGAACAGGA
    ACTGAAACAG ATACTTGTAC AGCTTGTGCA GGTACCTTGA TCCTTGAGTT CGCTGCTTTA
    AGTCGATTCA CAGGAGCAAC AATATTTGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC
    TGGGAAAAAA GACAGCGAAG TAGTAATTTA GTGGGTGTGA CTATAAATAT TCATACTGGA
    GACTGGGTAA GAAAAGATAG TGGAGTTGGA GCAGGGATTG ATTCATATTA TGAATATCTG
    TTGAAAGCTT ATGTCTTGCT TGGAGATGAC AGTTTCCTGG AAAGATTTAA TACACACTAT
    GATGCCATAA TGAGGTACAT TAGCCAGCCA CCTCTTCTGC TTGATGTGCA TATCCACAAG
    CCAATGCTGA ATGCTCGGAC TTGGATGGAT GCATTGCTCG CCTTTTTTCC AGGTTTGCAG
    GTCTTAAAGG GGGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA TCAGGTGATT
    AAAAAACATA ACTTCCTACC AGAGGCATTT ACTACAGATT TCAGGGTACA TTGGGCTCAA
    CATCCTTTAA GACCAGAATT TGCAGAAAGT ACCTACTTCT TATATAAAGC TACAGGAGAT
    CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TAAATAAATA TGCTAGAGTG
    CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGTACTGGAA GTCATGAGGA CAGAATGGAT
    TCTTTCTTCT TGGCTGAGAT GTTTAAATAT CTTTACCTAT TATTTGCGGA TAAAGAAGAC
    ATTATTTTTG ACATAGAAGA TTACATCTTT ACAACAGAAG CTCATCTGTT ACCTCTTTGG
    CTCTCTACTA CAAATCAAAG CATCTCTAAG AAGAATACAA CTTCAGAATA TACAGAACTG
    GATGACAGTA ATTTTGATTG GACTTGTCCA AACACTCAGA TTCTCTTCCC TAATGACCCA
    TTATATGCTC AGAGCATTCG TGAACCCTTG AAAAATGTGG TGGATAAGAG CTGTCCTAGA
    GGCATCATCA GAGTCAGAGA GGAGAGTTTC AGGAGTGGAG CTAAACCCCC TCTGAGAGCC
    AGAGATTTCA TGGCCACTAA CCCTGAACAT TTAGAAATCT TGAAGAAGAT GGGGGTGAGT
    TTGATTCACC TCAAAGATGG GAGAGTCCAG TTGGTTCAAC ATGCAATCCA AGCTGCTAGT
    TCAATTGATG CTGAGGATGG TTTGAGGTTC ATGCAGGAGA TGATCGAATT GTCAAGTCAG
    CAACAAAAAG AACAGCAGCT GCCTCCACGA GCTGTTCAAA TTGTATCCCA CCCATTTTTT
    GGCAGGGTAG TATTGACTGC TGGACCAGCT CAGTTTGGGC TGGATCTGTC TAAACATAAA
    GAGACAAGAG GATTTGTTGC AAGCAGTAAA CCATACAATG GTTGTTCAGA GCTTACTAAC
    CCCGAGGCAG TGATGGGAAA AATCGCTTTG ATACAAAGAG GACAGTGCAT GTTTGCAGAA
    AAGGCACGCA ACATTCAGAA TGCTGGTGCC ATTGGTGGCA TTGTTATTGA TGACAATGAG
    GGGAGCAGCA GTGATACTGC CCCTCTGTTC CAGATGGCAG GTGATGGAAA AGACACAGAC
    GACATCAAGA TCCCCATGCT ATTCTTATTC AGTAAAGAAG GAAGTATCAT ATTGGATGCC
    ATCCGGGAAT ATGAGGAGGT GGAAGTACTC CTTTCTGACA AAGCAAAAGA TAGAGCAGCA
    ATATTAAAAG GTAAAATGAT TCCAAGCTAC ATTATTAATA GTAATCCTGA AATGGAAAAT
    GAAGAACAAC CATCCTCTGA AAATGATTCT CAGAATCAGA GTGCTGAACA GATTACATCA
    AGTTCTCAGG AGGTTGATTT GGTGGATCAA GAGTCTCCTG AGGAAAGTTC TCTAAACTCA
    CAACCAGAAT CATCATCTCC AACAGATACA GACAATGCTG CAAGTGTTTC TGCTTCTGAA
    CAGAATTCTA ACCCCGTAGA AAACCATGAG ACTACAAGTC TTGATGGTGA ATGTACAGAT
    TTAGATAACC AACTTCAAGA ACAATCAGAA ACCGAAGAAG ATTCCAATCC TAATGTTAGC
    TGGGGTAAAA AGGTCCAGCC TATAGACTCC ATATTAGCAG ACTGGAATGA AGATATAGAA
    GCATTTGAAA TGATGGAGAA AGACGAACTA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe09157
    Clone ID Related Accession (Same CDS sequence) XM_005622430.3 , XM_005622430.2
    Accession Version XM_005622430.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2853bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005622430.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGCGAAG CCCGCGGCGG CCGGGGCTGT GGGTCCTCGG TCCCCCAGCG AGCGCCATGG 
    AGCCTGGTGG CGGCGGCGGC CGCGCTCTGC CTGGTGGCGG CCACGTCCGT GTGGACGGCG
    GGGGCCGCGC CCATGAGTAG GGAGGAGAGA CAGAAGCTTG GGAATCAAGT ACTGGAAATG
    TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCTGATGA ACTCATGCCT
    TTAACCTGTA GAGGTCGTGT TAGAGGCCAG GAGCCAAGTC GGGGTGATGT TGATGATGCC
    TTGGGAAAAT TTTCCCTGAC ACTGATTGAT TCTTTGGATA CTCTTGTGGT ATTAAATAAA
    ACTAAAGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGAG ATGTTAATTT AGATAATGAT
    GTAGTTGTAT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC
    TCGCTGGCGA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAACTT
    CTCCAAATGG CGAAGCAATT GGGTTACAAA CTTTTACCAG CTTTCAATAC TACCAGTGGC
    CTTCCTTATC CAAGAATTAA TTTAAAATTT GGTATCAGAA AACCGGAAGC TCGAACAGGA
    ACTGAAACAG ATACTTGTAC AGCTTGTGCA GGTACCTTGA TCCTTGAGTT CGCTGCTTTA
    AGTCGATTCA CAGGAGCAAC AATATTTGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC
    TGGGAAAAAA GACAGCGAAG TAGTAATTTA GTGGGTGTGA CTATAAATAT TCATACTGGA
    GACTGGGTAA GAAAAGATAG TGGAGTTGGA GCAGGGATTG ATTCATATTA TGAATATCTG
    TTGAAAGCTT ATGTCTTGCT TGGAGATGAC AGTTTCCTGG AAAGATTTAA TACACACTAT
    GATGCCATAA TGAGGTACAT TAGCCAGCCA CCTCTTCTGC TTGATGTGCA TATCCACAAG
    CCAATGCTGA ATGCTCGGAC TTGGATGGAT GCATTGCTCG CCTTTTTTCC AGGTTTGCAG
    GTCTTAAAGG GGGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA TCAGGTGATT
    AAAAAACATA ACTTCCTACC AGAGGCATTT ACTACAGATT TCAGGGTACA TTGGGCTCAA
    CATCCTTTAA GACCAGAATT TGCAGAAAGT ACCTACTTCT TATATAAAGC TACAGGAGAT
    CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TAAATAAATA TGCTAGAGTG
    CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGTACTGGAA GTCATGAGGA CAGAATGGAT
    TCTTTCTTCT TGGCTGAGAT GTTTAAATAT CTTTACCTAT TATTTGCGGA TAAAGAAGAC
    ATTATTTTTG ACATAGAAGA TTACATCTTT ACAACAGAAG CTCATCTGTT ACCTCTTTGG
    CTCTCTACTA CAAATCAAAG CATCTCTAAG AAGAATACAA CTTCAGAATA TACAGAACTG
    GATGACAGTA ATTTTGATTG GACTTGTCCA AACACTCAGA TTCTCTTCCC TAATGACCCA
    TTATATGCTC AGAGCATTCG TGAACCCTTG AAAAATGTGG TGGATAAGAG CTGTCCTAGA
    GGCATCATCA GAGTCAGAGA GGAGAGTTTC AGGAGTGGAG CTAAACCCCC TCTGAGAGCC
    AGAGATTTCA TGGCCACTAA CCCTGAACAT TTAGAAATCT TGAAGAAGAT GGGGGTGAGT
    TTGATTCACC TCAAAGATGG GAGAGTCCAG TTGGTTCAAC ATGCAATCCA AGCTGCTAGT
    TCAATTGATG CTGAGGATGG TTTGAGGTTC ATGCAGGAGA TGATCGAATT GTCAAGTCAG
    CAACAAAAAG AACAGCAGCT GCCTCCACGA GCTGTTCAAA TTGTATCCCA CCCATTTTTT
    GGCAGGGTAG TATTGACTGC TGGACCAGCT CAGTTTGGGC TGGATCTGTC TAAACATAAA
    GAGACAAGAG GATTTGTTGC AAGCAGTAAA CCATACAATG GTTGTTCAGA GCTTACTAAC
    CCCGAGGCAG TGATGGGAAA AATCGCTTTG ATACAAAGAG GACAGTGCAT GTTTGCAGAA
    AAGGCACGCA ACATTCAGAA TGCTGGTGCC ATTGGTGGCA TTGTTATTGA TGACAATGAG
    GGGAGCAGCA GTGATACTGC CCCTCTGTTC CAGATGGCAG GTGATGGAAA AGACACAGAC
    GACATCAAGA TCCCCATGCT ATTCTTATTC AGTAAAGAAG GAAGTATCAT ATTGGATGCC
    ATCCGGGAAT ATGAGGAGGT GGAAGTACTC CTTTCTGACA AAGCAAAAGA TAGAGCAGCA
    ATATTAAAAG GTAAAATGAT TCCAAGCTAC ATTATTAATA GTAATCCTGA AATGGAAAAT
    GAAGAACAAC CATCCTCTGA AAATGATTCT CAGAATCAGA GTGCTGAACA GATTACATCA
    AGTTCTCAGG AGGTTGATTT GGTGGATCAA GAGTCTCCTG AGGAAAGTTC TCTAAACTCA
    CAACCAGAAT CATCATCTCC AACAGATACA GACAATGCTG CAAGTGTTTC TGCTTCTGAA
    CAGAATTCTA ACCCCGTAGA AAACCATGAG ACTACAAGTC TTGATGGTGA ATGTACAGAT
    TTAGATAACC AACTTCAAGA ACAATCAGAA ACCGAAGAAG ATTCCAATCC TAATGTTAGC
    TGGGGTAAAA AGGTCCAGCC TATAGACTCC ATATTAGCAG ACTGGAATGA AGATATAGAA
    GCATTTGAAA TGATGGAGAA AGACGAACTA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005622487.1
    CDS224..3076
    Translation

    Target ORF information:

    RefSeq Version XM_005622430.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ER degradation enhancer, mannosidase alpha-like 3 (EDEM3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005622430.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGCGAAG CCCGCGGCGG CCGGGGCTGT GGGTCCTCGG TCCCCCAGCG AGCGCCATGG 
    AGCCTGGTGG CGGCGGCGGC CGCGCTCTGC CTGGTGGCGG CCACGTCCGT GTGGACGGCG
    GGGGCCGCGC CCATGAGTAG GGAGGAGAGA CAGAAGCTTG GGAATCAAGT ACTGGAAATG
    TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCTGATGA ACTCATGCCT
    TTAACCTGTA GAGGTCGTGT TAGAGGCCAG GAGCCAAGTC GGGGTGATGT TGATGATGCC
    TTGGGAAAAT TTTCCCTGAC ACTGATTGAT TCTTTGGATA CTCTTGTGGT ATTAAATAAA
    ACTAAAGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGAG ATGTTAATTT AGATAATGAT
    GTAGTTGTAT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC
    TCGCTGGCGA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAACTT
    CTCCAAATGG CGAAGCAATT GGGTTACAAA CTTTTACCAG CTTTCAATAC TACCAGTGGC
    CTTCCTTATC CAAGAATTAA TTTAAAATTT GGTATCAGAA AACCGGAAGC TCGAACAGGA
    ACTGAAACAG ATACTTGTAC AGCTTGTGCA GGTACCTTGA TCCTTGAGTT CGCTGCTTTA
    AGTCGATTCA CAGGAGCAAC AATATTTGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC
    TGGGAAAAAA GACAGCGAAG TAGTAATTTA GTGGGTGTGA CTATAAATAT TCATACTGGA
    GACTGGGTAA GAAAAGATAG TGGAGTTGGA GCAGGGATTG ATTCATATTA TGAATATCTG
    TTGAAAGCTT ATGTCTTGCT TGGAGATGAC AGTTTCCTGG AAAGATTTAA TACACACTAT
    GATGCCATAA TGAGGTACAT TAGCCAGCCA CCTCTTCTGC TTGATGTGCA TATCCACAAG
    CCAATGCTGA ATGCTCGGAC TTGGATGGAT GCATTGCTCG CCTTTTTTCC AGGTTTGCAG
    GTCTTAAAGG GGGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA TCAGGTGATT
    AAAAAACATA ACTTCCTACC AGAGGCATTT ACTACAGATT TCAGGGTACA TTGGGCTCAA
    CATCCTTTAA GACCAGAATT TGCAGAAAGT ACCTACTTCT TATATAAAGC TACAGGAGAT
    CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TAAATAAATA TGCTAGAGTG
    CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGTACTGGAA GTCATGAGGA CAGAATGGAT
    TCTTTCTTCT TGGCTGAGAT GTTTAAATAT CTTTACCTAT TATTTGCGGA TAAAGAAGAC
    ATTATTTTTG ACATAGAAGA TTACATCTTT ACAACAGAAG CTCATCTGTT ACCTCTTTGG
    CTCTCTACTA CAAATCAAAG CATCTCTAAG AAGAATACAA CTTCAGAATA TACAGAACTG
    GATGACAGTA ATTTTGATTG GACTTGTCCA AACACTCAGA TTCTCTTCCC TAATGACCCA
    TTATATGCTC AGAGCATTCG TGAACCCTTG AAAAATGTGG TGGATAAGAG CTGTCCTAGA
    GGCATCATCA GAGTCAGAGA GGAGAGTTTC AGGAGTGGAG CTAAACCCCC TCTGAGAGCC
    AGAGATTTCA TGGCCACTAA CCCTGAACAT TTAGAAATCT TGAAGAAGAT GGGGGTGAGT
    TTGATTCACC TCAAAGATGG GAGAGTCCAG TTGGTTCAAC ATGCAATCCA AGCTGCTAGT
    TCAATTGATG CTGAGGATGG TTTGAGGTTC ATGCAGGAGA TGATCGAATT GTCAAGTCAG
    CAACAAAAAG AACAGCAGCT GCCTCCACGA GCTGTTCAAA TTGTATCCCA CCCATTTTTT
    GGCAGGGTAG TATTGACTGC TGGACCAGCT CAGTTTGGGC TGGATCTGTC TAAACATAAA
    GAGACAAGAG GATTTGTTGC AAGCAGTAAA CCATACAATG GTTGTTCAGA GCTTACTAAC
    CCCGAGGCAG TGATGGGAAA AATCGCTTTG ATACAAAGAG GACAGTGCAT GTTTGCAGAA
    AAGGCACGCA ACATTCAGAA TGCTGGTGCC ATTGGTGGCA TTGTTATTGA TGACAATGAG
    GGGAGCAGCA GTGATACTGC CCCTCTGTTC CAGATGGCAG GTGATGGAAA AGACACAGAC
    GACATCAAGA TCCCCATGCT ATTCTTATTC AGTAAAGAAG GAAGTATCAT ATTGGATGCC
    ATCCGGGAAT ATGAGGAGGT GGAAGTACTC CTTTCTGACA AAGCAAAAGA TAGAGCAGCA
    ATATTAAAAG GTAAAATGAT TCCAAGCTAC ATTATTAATA GTAATCCTGA AATGGAAAAT
    GAAGAACAAC CATCCTCTGA AAATGATTCT CAGAATCAGA GTGCTGAACA GATTACATCA
    AGTTCTCAGG AGGTTGATTT GGTGGATCAA GAGTCTCCTG AGGAAAGTTC TCTAAACTCA
    CAACCAGAAT CATCATCTCC AACAGATACA GACAATGCTG CAAGTGTTTC TGCTTCTGAA
    CAGAATTCTA ACCCCGTAGA AAACCATGAG ACTACAAGTC TTGATGGTGA ATGTACAGAT
    TTAGATAACC AACTTCAAGA ACAATCAGAA ACCGAAGAAG ATTCCAATCC TAATGTTAGC
    TGGGGTAAAA AGGTCCAGCC TATAGACTCC ATATTAGCAG ACTGGAATGA AGATATAGAA
    GCATTTGAAA TGATGGAGAA AGACGAACTA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe09158
    Clone ID Related Accession (Same CDS sequence) XM_003434974.4 , XM_003434974.3
    Accession Version XM_003434974.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2850bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003434974.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGCGAAG CCCGCGGCGG CCGGGGCTGT GGGTCCTCGG TCCCCCAGCG AGCGCCATGG 
    AGCCTGGTGG CGGCGGCGGC CGCGCTCTGC CTGGTGGCGG CCACGTCCGT GTGGACGGCG
    GGGGCCGCGC CCATGAGTAG GGAGGAGAGA CAGAAGCTTG GGAATCAAGT ACTGGAAATG
    TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCTGATGA ACTCATGCCT
    TTAACCTGTA GAGGTCGTGT TAGAGGCCAG GAGCCAAGTC GGGGTGATGT TGATGATGCC
    TTGGGAAAAT TTTCCCTGAC ACTGATTGAT TCTTTGGATA CTCTTGTGGT ATTAAATAAA
    ACTAAAGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGAG ATGTTAATTT AGATAATGAT
    GTAGTTGTAT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC
    TCGCTGGCGA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAACTT
    CTCCAAATGG CGAAGCAATT GGGTTACAAA CTTTTACCAG CTTTCAATAC TACCAGTGGC
    CTTCCTTATC CAAGAATTAA TTTAAAATTT GGTATCAGAA AACCGGAAGC TCGAACAGGA
    ACTGAAACAG ATACTTGTAC AGCTTGTGCA GGTACCTTGA TCCTTGAGTT CGCTGCTTTA
    AGTCGATTCA CAGGAGCAAC AATATTTGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC
    TGGGAAAAAA GACAGCGAAG TAGTAATTTA GTGGGTGTGA CTATAAATAT TCATACTGGA
    GACTGGGTAA GAAAAGATAG TGGAGTTGGA GCAGGGATTG ATTCATATTA TGAATATCTG
    TTGAAAGCTT ATGTCTTGCT TGGAGATGAC AGTTTCCTGG AAAGATTTAA TACACACTAT
    GATGCCATAA TGAGGTACAT TAGCCAGCCA CCTCTTCTGC TTGATGTGCA TATCCACAAG
    CCAATGCTGA ATGCTCGGAC TTGGATGGAT GCATTGCTCG CCTTTTTTCC AGGTTTGCAG
    GTCTTAAAGG GGGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA TCAGGTGATT
    AAAAAACATA ACTTCCTACC AGAGGCATTT ACTACAGATT TCAGGGTACA TTGGGCTCAA
    CATCCTTTAA GACCAGAATT TGCAGAAAGT ACCTACTTCT TATATAAAGC TACAGGAGAT
    CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TAAATAAATA TGCTAGAGTG
    CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGTACTGGAA GTCATGAGGA CAGAATGGAT
    TCTTTCTTCT TGGCTGAGAT GTTTAAATAT CTTTACCTAT TATTTGCGGA TAAAGAAGAC
    ATTATTTTTG ACATAGAAGA TTACATCTTT ACAACAGAAG CTCATCTGTT ACCTCTTTGG
    CTCTCTACTA CAAATCAAAG CATCTCTAAG AAGAATACAA CTTCAGAATA TACAGAACTG
    GATGACAGTA ATTTTGATTG GACTTGTCCA AACACTCAGA TTCTCTTCCC TAATGACCCA
    TTATATGCTC AGAGCATTCG TGAACCCTTG AAAAATGTGG TGGATAAGAG CTGTCCTAGA
    GGCATCATCA GAGTAGAGGA GAGTTTCAGG AGTGGAGCTA AACCCCCTCT GAGAGCCAGA
    GATTTCATGG CCACTAACCC TGAACATTTA GAAATCTTGA AGAAGATGGG GGTGAGTTTG
    ATTCACCTCA AAGATGGGAG AGTCCAGTTG GTTCAACATG CAATCCAAGC TGCTAGTTCA
    ATTGATGCTG AGGATGGTTT GAGGTTCATG CAGGAGATGA TCGAATTGTC AAGTCAGCAA
    CAAAAAGAAC AGCAGCTGCC TCCACGAGCT GTTCAAATTG TATCCCACCC ATTTTTTGGC
    AGGGTAGTAT TGACTGCTGG ACCAGCTCAG TTTGGGCTGG ATCTGTCTAA ACATAAAGAG
    ACAAGAGGAT TTGTTGCAAG CAGTAAACCA TACAATGGTT GTTCAGAGCT TACTAACCCC
    GAGGCAGTGA TGGGAAAAAT CGCTTTGATA CAAAGAGGAC AGTGCATGTT TGCAGAAAAG
    GCACGCAACA TTCAGAATGC TGGTGCCATT GGTGGCATTG TTATTGATGA CAATGAGGGG
    AGCAGCAGTG ATACTGCCCC TCTGTTCCAG ATGGCAGGTG ATGGAAAAGA CACAGACGAC
    ATCAAGATCC CCATGCTATT CTTATTCAGT AAAGAAGGAA GTATCATATT GGATGCCATC
    CGGGAATATG AGGAGGTGGA AGTACTCCTT TCTGACAAAG CAAAAGATAG AGCAGCAATA
    TTAAAAGGTA AAATGATTCC AAGCTACATT ATTAATAGTA ATCCTGAAAT GGAAAATGAA
    GAACAACCAT CCTCTGAAAA TGATTCTCAG AATCAGAGTG CTGAACAGAT TACATCAAGT
    TCTCAGGAGG TTGATTTGGT GGATCAAGAG TCTCCTGAGG AAAGTTCTCT AAACTCACAA
    CCAGAATCAT CATCTCCAAC AGATACAGAC AATGCTGCAA GTGTTTCTGC TTCTGAACAG
    AATTCTAACC CCGTAGAAAA CCATGAGACT ACAAGTCTTG ATGGTGAATG TACAGATTTA
    GATAACCAAC TTCAAGAACA ATCAGAAACC GAAGAAGATT CCAATCCTAA TGTTAGCTGG
    GGTAAAAAGG TCCAGCCTAT AGACTCCATA TTAGCAGACT GGAATGAAGA TATAGAAGCA
    TTTGAAATGA TGGAGAAAGA CGAACTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003435022.2
    CDS220..3069
    Translation

    Target ORF information:

    RefSeq Version XM_003434974.4
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ER degradation enhancing alpha-mannosidase like protein 3 (EDEM3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003434974.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGCGAAG CCCGCGGCGG CCGGGGCTGT GGGTCCTCGG TCCCCCAGCG AGCGCCATGG 
    AGCCTGGTGG CGGCGGCGGC CGCGCTCTGC CTGGTGGCGG CCACGTCCGT GTGGACGGCG
    GGGGCCGCGC CCATGAGTAG GGAGGAGAGA CAGAAGCTTG GGAATCAAGT ACTGGAAATG
    TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCTGATGA ACTCATGCCT
    TTAACCTGTA GAGGTCGTGT TAGAGGCCAG GAGCCAAGTC GGGGTGATGT TGATGATGCC
    TTGGGAAAAT TTTCCCTGAC ACTGATTGAT TCTTTGGATA CTCTTGTGGT ATTAAATAAA
    ACTAAAGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGAG ATGTTAATTT AGATAATGAT
    GTAGTTGTAT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC
    TCGCTGGCGA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAACTT
    CTCCAAATGG CGAAGCAATT GGGTTACAAA CTTTTACCAG CTTTCAATAC TACCAGTGGC
    CTTCCTTATC CAAGAATTAA TTTAAAATTT GGTATCAGAA AACCGGAAGC TCGAACAGGA
    ACTGAAACAG ATACTTGTAC AGCTTGTGCA GGTACCTTGA TCCTTGAGTT CGCTGCTTTA
    AGTCGATTCA CAGGAGCAAC AATATTTGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC
    TGGGAAAAAA GACAGCGAAG TAGTAATTTA GTGGGTGTGA CTATAAATAT TCATACTGGA
    GACTGGGTAA GAAAAGATAG TGGAGTTGGA GCAGGGATTG ATTCATATTA TGAATATCTG
    TTGAAAGCTT ATGTCTTGCT TGGAGATGAC AGTTTCCTGG AAAGATTTAA TACACACTAT
    GATGCCATAA TGAGGTACAT TAGCCAGCCA CCTCTTCTGC TTGATGTGCA TATCCACAAG
    CCAATGCTGA ATGCTCGGAC TTGGATGGAT GCATTGCTCG CCTTTTTTCC AGGTTTGCAG
    GTCTTAAAGG GGGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA TCAGGTGATT
    AAAAAACATA ACTTCCTACC AGAGGCATTT ACTACAGATT TCAGGGTACA TTGGGCTCAA
    CATCCTTTAA GACCAGAATT TGCAGAAAGT ACCTACTTCT TATATAAAGC TACAGGAGAT
    CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TAAATAAATA TGCTAGAGTG
    CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGTACTGGAA GTCATGAGGA CAGAATGGAT
    TCTTTCTTCT TGGCTGAGAT GTTTAAATAT CTTTACCTAT TATTTGCGGA TAAAGAAGAC
    ATTATTTTTG ACATAGAAGA TTACATCTTT ACAACAGAAG CTCATCTGTT ACCTCTTTGG
    CTCTCTACTA CAAATCAAAG CATCTCTAAG AAGAATACAA CTTCAGAATA TACAGAACTG
    GATGACAGTA ATTTTGATTG GACTTGTCCA AACACTCAGA TTCTCTTCCC TAATGACCCA
    TTATATGCTC AGAGCATTCG TGAACCCTTG AAAAATGTGG TGGATAAGAG CTGTCCTAGA
    GGCATCATCA GAGTAGAGGA GAGTTTCAGG AGTGGAGCTA AACCCCCTCT GAGAGCCAGA
    GATTTCATGG CCACTAACCC TGAACATTTA GAAATCTTGA AGAAGATGGG GGTGAGTTTG
    ATTCACCTCA AAGATGGGAG AGTCCAGTTG GTTCAACATG CAATCCAAGC TGCTAGTTCA
    ATTGATGCTG AGGATGGTTT GAGGTTCATG CAGGAGATGA TCGAATTGTC AAGTCAGCAA
    CAAAAAGAAC AGCAGCTGCC TCCACGAGCT GTTCAAATTG TATCCCACCC ATTTTTTGGC
    AGGGTAGTAT TGACTGCTGG ACCAGCTCAG TTTGGGCTGG ATCTGTCTAA ACATAAAGAG
    ACAAGAGGAT TTGTTGCAAG CAGTAAACCA TACAATGGTT GTTCAGAGCT TACTAACCCC
    GAGGCAGTGA TGGGAAAAAT CGCTTTGATA CAAAGAGGAC AGTGCATGTT TGCAGAAAAG
    GCACGCAACA TTCAGAATGC TGGTGCCATT GGTGGCATTG TTATTGATGA CAATGAGGGG
    AGCAGCAGTG ATACTGCCCC TCTGTTCCAG ATGGCAGGTG ATGGAAAAGA CACAGACGAC
    ATCAAGATCC CCATGCTATT CTTATTCAGT AAAGAAGGAA GTATCATATT GGATGCCATC
    CGGGAATATG AGGAGGTGGA AGTACTCCTT TCTGACAAAG CAAAAGATAG AGCAGCAATA
    TTAAAAGGTA AAATGATTCC AAGCTACATT ATTAATAGTA ATCCTGAAAT GGAAAATGAA
    GAACAACCAT CCTCTGAAAA TGATTCTCAG AATCAGAGTG CTGAACAGAT TACATCAAGT
    TCTCAGGAGG TTGATTTGGT GGATCAAGAG TCTCCTGAGG AAAGTTCTCT AAACTCACAA
    CCAGAATCAT CATCTCCAAC AGATACAGAC AATGCTGCAA GTGTTTCTGC TTCTGAACAG
    AATTCTAACC CCGTAGAAAA CCATGAGACT ACAAGTCTTG ATGGTGAATG TACAGATTTA
    GATAACCAAC TTCAAGAACA ATCAGAAACC GAAGAAGATT CCAATCCTAA TGTTAGCTGG
    GGTAAAAAGG TCCAGCCTAT AGACTCCATA TTAGCAGACT GGAATGAAGA TATAGAAGCA
    TTTGAAATGA TGGAGAAAGA CGAACTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe09158
    Clone ID Related Accession (Same CDS sequence) XM_003434974.4 , XM_003434974.3
    Accession Version XM_003434974.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2850bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003434974.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGCGAAG CCCGCGGCGG CCGGGGCTGT GGGTCCTCGG TCCCCCAGCG AGCGCCATGG 
    AGCCTGGTGG CGGCGGCGGC CGCGCTCTGC CTGGTGGCGG CCACGTCCGT GTGGACGGCG
    GGGGCCGCGC CCATGAGTAG GGAGGAGAGA CAGAAGCTTG GGAATCAAGT ACTGGAAATG
    TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCTGATGA ACTCATGCCT
    TTAACCTGTA GAGGTCGTGT TAGAGGCCAG GAGCCAAGTC GGGGTGATGT TGATGATGCC
    TTGGGAAAAT TTTCCCTGAC ACTGATTGAT TCTTTGGATA CTCTTGTGGT ATTAAATAAA
    ACTAAAGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGAG ATGTTAATTT AGATAATGAT
    GTAGTTGTAT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC
    TCGCTGGCGA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAACTT
    CTCCAAATGG CGAAGCAATT GGGTTACAAA CTTTTACCAG CTTTCAATAC TACCAGTGGC
    CTTCCTTATC CAAGAATTAA TTTAAAATTT GGTATCAGAA AACCGGAAGC TCGAACAGGA
    ACTGAAACAG ATACTTGTAC AGCTTGTGCA GGTACCTTGA TCCTTGAGTT CGCTGCTTTA
    AGTCGATTCA CAGGAGCAAC AATATTTGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC
    TGGGAAAAAA GACAGCGAAG TAGTAATTTA GTGGGTGTGA CTATAAATAT TCATACTGGA
    GACTGGGTAA GAAAAGATAG TGGAGTTGGA GCAGGGATTG ATTCATATTA TGAATATCTG
    TTGAAAGCTT ATGTCTTGCT TGGAGATGAC AGTTTCCTGG AAAGATTTAA TACACACTAT
    GATGCCATAA TGAGGTACAT TAGCCAGCCA CCTCTTCTGC TTGATGTGCA TATCCACAAG
    CCAATGCTGA ATGCTCGGAC TTGGATGGAT GCATTGCTCG CCTTTTTTCC AGGTTTGCAG
    GTCTTAAAGG GGGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA TCAGGTGATT
    AAAAAACATA ACTTCCTACC AGAGGCATTT ACTACAGATT TCAGGGTACA TTGGGCTCAA
    CATCCTTTAA GACCAGAATT TGCAGAAAGT ACCTACTTCT TATATAAAGC TACAGGAGAT
    CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TAAATAAATA TGCTAGAGTG
    CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGTACTGGAA GTCATGAGGA CAGAATGGAT
    TCTTTCTTCT TGGCTGAGAT GTTTAAATAT CTTTACCTAT TATTTGCGGA TAAAGAAGAC
    ATTATTTTTG ACATAGAAGA TTACATCTTT ACAACAGAAG CTCATCTGTT ACCTCTTTGG
    CTCTCTACTA CAAATCAAAG CATCTCTAAG AAGAATACAA CTTCAGAATA TACAGAACTG
    GATGACAGTA ATTTTGATTG GACTTGTCCA AACACTCAGA TTCTCTTCCC TAATGACCCA
    TTATATGCTC AGAGCATTCG TGAACCCTTG AAAAATGTGG TGGATAAGAG CTGTCCTAGA
    GGCATCATCA GAGTAGAGGA GAGTTTCAGG AGTGGAGCTA AACCCCCTCT GAGAGCCAGA
    GATTTCATGG CCACTAACCC TGAACATTTA GAAATCTTGA AGAAGATGGG GGTGAGTTTG
    ATTCACCTCA AAGATGGGAG AGTCCAGTTG GTTCAACATG CAATCCAAGC TGCTAGTTCA
    ATTGATGCTG AGGATGGTTT GAGGTTCATG CAGGAGATGA TCGAATTGTC AAGTCAGCAA
    CAAAAAGAAC AGCAGCTGCC TCCACGAGCT GTTCAAATTG TATCCCACCC ATTTTTTGGC
    AGGGTAGTAT TGACTGCTGG ACCAGCTCAG TTTGGGCTGG ATCTGTCTAA ACATAAAGAG
    ACAAGAGGAT TTGTTGCAAG CAGTAAACCA TACAATGGTT GTTCAGAGCT TACTAACCCC
    GAGGCAGTGA TGGGAAAAAT CGCTTTGATA CAAAGAGGAC AGTGCATGTT TGCAGAAAAG
    GCACGCAACA TTCAGAATGC TGGTGCCATT GGTGGCATTG TTATTGATGA CAATGAGGGG
    AGCAGCAGTG ATACTGCCCC TCTGTTCCAG ATGGCAGGTG ATGGAAAAGA CACAGACGAC
    ATCAAGATCC CCATGCTATT CTTATTCAGT AAAGAAGGAA GTATCATATT GGATGCCATC
    CGGGAATATG AGGAGGTGGA AGTACTCCTT TCTGACAAAG CAAAAGATAG AGCAGCAATA
    TTAAAAGGTA AAATGATTCC AAGCTACATT ATTAATAGTA ATCCTGAAAT GGAAAATGAA
    GAACAACCAT CCTCTGAAAA TGATTCTCAG AATCAGAGTG CTGAACAGAT TACATCAAGT
    TCTCAGGAGG TTGATTTGGT GGATCAAGAG TCTCCTGAGG AAAGTTCTCT AAACTCACAA
    CCAGAATCAT CATCTCCAAC AGATACAGAC AATGCTGCAA GTGTTTCTGC TTCTGAACAG
    AATTCTAACC CCGTAGAAAA CCATGAGACT ACAAGTCTTG ATGGTGAATG TACAGATTTA
    GATAACCAAC TTCAAGAACA ATCAGAAACC GAAGAAGATT CCAATCCTAA TGTTAGCTGG
    GGTAAAAAGG TCCAGCCTAT AGACTCCATA TTAGCAGACT GGAATGAAGA TATAGAAGCA
    TTTGAAATGA TGGAGAAAGA CGAACTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003435022.2
    CDS224..3073
    Translation

    Target ORF information:

    RefSeq Version XM_003434974.3
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ER degradation enhancer, mannosidase alpha-like 3 (EDEM3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003434974.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGCGAAG CCCGCGGCGG CCGGGGCTGT GGGTCCTCGG TCCCCCAGCG AGCGCCATGG 
    AGCCTGGTGG CGGCGGCGGC CGCGCTCTGC CTGGTGGCGG CCACGTCCGT GTGGACGGCG
    GGGGCCGCGC CCATGAGTAG GGAGGAGAGA CAGAAGCTTG GGAATCAAGT ACTGGAAATG
    TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCTGATGA ACTCATGCCT
    TTAACCTGTA GAGGTCGTGT TAGAGGCCAG GAGCCAAGTC GGGGTGATGT TGATGATGCC
    TTGGGAAAAT TTTCCCTGAC ACTGATTGAT TCTTTGGATA CTCTTGTGGT ATTAAATAAA
    ACTAAAGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGAG ATGTTAATTT AGATAATGAT
    GTAGTTGTAT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC
    TCGCTGGCGA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAACTT
    CTCCAAATGG CGAAGCAATT GGGTTACAAA CTTTTACCAG CTTTCAATAC TACCAGTGGC
    CTTCCTTATC CAAGAATTAA TTTAAAATTT GGTATCAGAA AACCGGAAGC TCGAACAGGA
    ACTGAAACAG ATACTTGTAC AGCTTGTGCA GGTACCTTGA TCCTTGAGTT CGCTGCTTTA
    AGTCGATTCA CAGGAGCAAC AATATTTGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC
    TGGGAAAAAA GACAGCGAAG TAGTAATTTA GTGGGTGTGA CTATAAATAT TCATACTGGA
    GACTGGGTAA GAAAAGATAG TGGAGTTGGA GCAGGGATTG ATTCATATTA TGAATATCTG
    TTGAAAGCTT ATGTCTTGCT TGGAGATGAC AGTTTCCTGG AAAGATTTAA TACACACTAT
    GATGCCATAA TGAGGTACAT TAGCCAGCCA CCTCTTCTGC TTGATGTGCA TATCCACAAG
    CCAATGCTGA ATGCTCGGAC TTGGATGGAT GCATTGCTCG CCTTTTTTCC AGGTTTGCAG
    GTCTTAAAGG GGGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA TCAGGTGATT
    AAAAAACATA ACTTCCTACC AGAGGCATTT ACTACAGATT TCAGGGTACA TTGGGCTCAA
    CATCCTTTAA GACCAGAATT TGCAGAAAGT ACCTACTTCT TATATAAAGC TACAGGAGAT
    CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TAAATAAATA TGCTAGAGTG
    CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGTACTGGAA GTCATGAGGA CAGAATGGAT
    TCTTTCTTCT TGGCTGAGAT GTTTAAATAT CTTTACCTAT TATTTGCGGA TAAAGAAGAC
    ATTATTTTTG ACATAGAAGA TTACATCTTT ACAACAGAAG CTCATCTGTT ACCTCTTTGG
    CTCTCTACTA CAAATCAAAG CATCTCTAAG AAGAATACAA CTTCAGAATA TACAGAACTG
    GATGACAGTA ATTTTGATTG GACTTGTCCA AACACTCAGA TTCTCTTCCC TAATGACCCA
    TTATATGCTC AGAGCATTCG TGAACCCTTG AAAAATGTGG TGGATAAGAG CTGTCCTAGA
    GGCATCATCA GAGTAGAGGA GAGTTTCAGG AGTGGAGCTA AACCCCCTCT GAGAGCCAGA
    GATTTCATGG CCACTAACCC TGAACATTTA GAAATCTTGA AGAAGATGGG GGTGAGTTTG
    ATTCACCTCA AAGATGGGAG AGTCCAGTTG GTTCAACATG CAATCCAAGC TGCTAGTTCA
    ATTGATGCTG AGGATGGTTT GAGGTTCATG CAGGAGATGA TCGAATTGTC AAGTCAGCAA
    CAAAAAGAAC AGCAGCTGCC TCCACGAGCT GTTCAAATTG TATCCCACCC ATTTTTTGGC
    AGGGTAGTAT TGACTGCTGG ACCAGCTCAG TTTGGGCTGG ATCTGTCTAA ACATAAAGAG
    ACAAGAGGAT TTGTTGCAAG CAGTAAACCA TACAATGGTT GTTCAGAGCT TACTAACCCC
    GAGGCAGTGA TGGGAAAAAT CGCTTTGATA CAAAGAGGAC AGTGCATGTT TGCAGAAAAG
    GCACGCAACA TTCAGAATGC TGGTGCCATT GGTGGCATTG TTATTGATGA CAATGAGGGG
    AGCAGCAGTG ATACTGCCCC TCTGTTCCAG ATGGCAGGTG ATGGAAAAGA CACAGACGAC
    ATCAAGATCC CCATGCTATT CTTATTCAGT AAAGAAGGAA GTATCATATT GGATGCCATC
    CGGGAATATG AGGAGGTGGA AGTACTCCTT TCTGACAAAG CAAAAGATAG AGCAGCAATA
    TTAAAAGGTA AAATGATTCC AAGCTACATT ATTAATAGTA ATCCTGAAAT GGAAAATGAA
    GAACAACCAT CCTCTGAAAA TGATTCTCAG AATCAGAGTG CTGAACAGAT TACATCAAGT
    TCTCAGGAGG TTGATTTGGT GGATCAAGAG TCTCCTGAGG AAAGTTCTCT AAACTCACAA
    CCAGAATCAT CATCTCCAAC AGATACAGAC AATGCTGCAA GTGTTTCTGC TTCTGAACAG
    AATTCTAACC CCGTAGAAAA CCATGAGACT ACAAGTCTTG ATGGTGAATG TACAGATTTA
    GATAACCAAC TTCAAGAACA ATCAGAAACC GAAGAAGATT CCAATCCTAA TGTTAGCTGG
    GGTAAAAAGG TCCAGCCTAT AGACTCCATA TTAGCAGACT GGAATGAAGA TATAGAAGCA
    TTTGAAATGA TGGAGAAAGA CGAACTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe09159
    Clone ID Related Accession (Same CDS sequence) XM_005622431.3 , XM_005622431.2
    Accession Version XM_005622431.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2805bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005622431.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGCGAAG CCCGCGGCGG CCGGGGCTGT GGGTCCTCGG TCCCCCAGCG AGCGCCATGG 
    AGCCTGGTGG CGGCGGCGGC CGCGCTCTGC CTGGTGGCGG CCACGTCCGT GTGGACGGCG
    GGGGCCGCGC CCATGAGTAG GGAGGAGAGA CAGAAGCTTG GGAATCAAGT ACTGGAAATG
    TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCTGATGA ACTCATGCCT
    TTAACCTGTA GAGGTCGTGT TAGAGGCCAG GAGCCAAGTC GGGGTGATGT TGATGATGCC
    TTGGGAAAAT TTTCCCTGAC ACTGATTGAT TCTTTGGATA CTCTTGTGGT ATTAAATAAA
    ACTAAAGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGAG ATGTTAATTT AGATAATGAT
    GTAGTTGTAT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC
    TCGCTGGCGA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAACTT
    CTCCAAATGG CGAAGCAATT GGGTTACAAA CTTTTACCAG CTTTCAATAC TACCAGTGGC
    CTTCCTTATC CAAGAATTAA TTTAAAATTT GGTATCAGAA AACCGGAAGC TCGAACAGGA
    ACTGAAACAG ATACTTGTAC AGCTTGTGCA GGTACCTTGA TCCTTGAGTT CGCTGCTTTA
    AGTCGATTCA CAGGAGCAAC AATATTTGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC
    TGGGAAAAAA GACAGCGAAG TAGTAATTTA GTGGGTGTGA CTATAAATAT TCATACTGGA
    GACTGGGTAA GAAAAGATAG TGGAGTTGGA GCAGGGATTG ATTCATATTA TGAATATCTG
    TTGAAAGCTT ATGTCTTGCT TGGAGATGAC AGTTTCCTGG AAAGATTTAA TACACACTAT
    GATGCCATAA TGAGGTACAT TAGCCAGCCA CCTCTTCTGC TTGATGTGCA TATCCACAAG
    CCAATGCTGA ATGCTCGGAC TTGGATGGAT GCATTGCTCG CCTTTTTTCC AGGTTTGCAG
    GTCTTAAAGG GGGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA TCAGGTGATT
    AAAAAACATA ACTTCCTACC AGAGGCATTT ACTACAGATT TCAGGGTACA TTGGGCTCAA
    CATCCTTTAA GACCAGAATT TGCAGAAAGT ACCTACTTCT TATATAAAGC TACAGGAGAT
    CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TAAATAAATA TGCTAGAGTG
    CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGTACTGGAA GTCATGAGGA CAGAATGGAT
    TCTTTCTTCT TGGCTGAGAT GTTTAAATAT CTTTACCTAT TATTTGCGGA TAAAGAAGAC
    ATTATTTTTG ACATAGAAGA TTACATCTTT ACAACAGAAG CTCATCTGTT ACCTCTTTGG
    CTCTCTACTA CAAATCAAAG CATCTCTAAG AAGAATACAA CTTCAGAATA TACAGAACTG
    GATGACAGTA ATTTTGATTG GACTTGTCCA AACACTCAGA TTCTCTTCCC TAATGACCCA
    TTATATGCTC AGAGCATTCG TGAACCCTTG AAAAATGTGG TGGATAAGAG CTGTCCTAGA
    GGCATCATCA GAGTCAGAGA GGAGAGTTTC AGGAGTGGAG CTAAACCCCC TCTGAGAGCC
    AGAGATTTCA TGGCCACTAA CCCTGAACAT TTAGAAATCT TGAAGAAGAT GGGGGTGAGT
    TTGATTCACC TCAAAGATGG GAGAGTCCAG TTGGTTCAAC ATGCAATCCA AGCTGCTAGT
    TCAATTGATG CTGAGGATGG TTTGAGGTTC ATGCAGGAGA TGATCGAATT GTCAAGTCAG
    CAACAAAAAG AACAGCAGCT GCCTCCACGA GCTGTTCAAA TTGTATCCCA CCCATTTTTT
    GGCAGGGTAG TATTGACTGC TGGACCAGCT CAGTTTGGGC TGGATCTGTC TAAACATAAA
    GAGACAAGAG GATTTGTTGC AAGCAGTAAA CCATACAATG GTTGTTCAGA GCTTACTAAC
    CCCGAGGCAG TGATGGGAAA AATCGCTTTG ATACAAAGAG GACAGTGCAT GTTTGCAGAA
    AAGGCACGCA ACATTCAGAA TGCTGGTGCC ATTGGTGGCA TTGTTATTGA TGACAATGAG
    GGGAGCAGCA GTGATACTGC CCCTCTGTTC CAGATGGCAG GTGATGGAAA AGACACAGAC
    GACATCAAGA TCCCCATGCT ATTCTTATTC AGTAAAGAAG GAAGTATCAT ATTGGATGCC
    ATCCGGGAAT ATGAGGAGGT GGAAGTACTC CTTTCTGACA AAGCAAAAGA TAGAGATCCT
    GAAATGGAAA ATGAAGAACA ACCATCCTCT GAAAATGATT CTCAGAATCA GAGTGCTGAA
    CAGATTACAT CAAGTTCTCA GGAGGTTGAT TTGGTGGATC AAGAGTCTCC TGAGGAAAGT
    TCTCTAAACT CACAACCAGA ATCATCATCT CCAACAGATA CAGACAATGC TGCAAGTGTT
    TCTGCTTCTG AACAGAATTC TAACCCCGTA GAAAACCATG AGACTACAAG TCTTGATGGT
    GAATGTACAG ATTTAGATAA CCAACTTCAA GAACAATCAG AAACCGAAGA AGATTCCAAT
    CCTAATGTTA GCTGGGGTAA AAAGGTCCAG CCTATAGACT CCATATTAGC AGACTGGAAT
    GAAGATATAG AAGCATTTGA AATGATGGAG AAAGACGAAC TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005622488.1
    CDS220..3024
    Translation

    Target ORF information:

    RefSeq Version XM_005622431.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ER degradation enhancing alpha-mannosidase like protein 3 (EDEM3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005622431.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGCGAAG CCCGCGGCGG CCGGGGCTGT GGGTCCTCGG TCCCCCAGCG AGCGCCATGG 
    AGCCTGGTGG CGGCGGCGGC CGCGCTCTGC CTGGTGGCGG CCACGTCCGT GTGGACGGCG
    GGGGCCGCGC CCATGAGTAG GGAGGAGAGA CAGAAGCTTG GGAATCAAGT ACTGGAAATG
    TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCTGATGA ACTCATGCCT
    TTAACCTGTA GAGGTCGTGT TAGAGGCCAG GAGCCAAGTC GGGGTGATGT TGATGATGCC
    TTGGGAAAAT TTTCCCTGAC ACTGATTGAT TCTTTGGATA CTCTTGTGGT ATTAAATAAA
    ACTAAAGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGAG ATGTTAATTT AGATAATGAT
    GTAGTTGTAT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC
    TCGCTGGCGA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAACTT
    CTCCAAATGG CGAAGCAATT GGGTTACAAA CTTTTACCAG CTTTCAATAC TACCAGTGGC
    CTTCCTTATC CAAGAATTAA TTTAAAATTT GGTATCAGAA AACCGGAAGC TCGAACAGGA
    ACTGAAACAG ATACTTGTAC AGCTTGTGCA GGTACCTTGA TCCTTGAGTT CGCTGCTTTA
    AGTCGATTCA CAGGAGCAAC AATATTTGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC
    TGGGAAAAAA GACAGCGAAG TAGTAATTTA GTGGGTGTGA CTATAAATAT TCATACTGGA
    GACTGGGTAA GAAAAGATAG TGGAGTTGGA GCAGGGATTG ATTCATATTA TGAATATCTG
    TTGAAAGCTT ATGTCTTGCT TGGAGATGAC AGTTTCCTGG AAAGATTTAA TACACACTAT
    GATGCCATAA TGAGGTACAT TAGCCAGCCA CCTCTTCTGC TTGATGTGCA TATCCACAAG
    CCAATGCTGA ATGCTCGGAC TTGGATGGAT GCATTGCTCG CCTTTTTTCC AGGTTTGCAG
    GTCTTAAAGG GGGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA TCAGGTGATT
    AAAAAACATA ACTTCCTACC AGAGGCATTT ACTACAGATT TCAGGGTACA TTGGGCTCAA
    CATCCTTTAA GACCAGAATT TGCAGAAAGT ACCTACTTCT TATATAAAGC TACAGGAGAT
    CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TAAATAAATA TGCTAGAGTG
    CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGTACTGGAA GTCATGAGGA CAGAATGGAT
    TCTTTCTTCT TGGCTGAGAT GTTTAAATAT CTTTACCTAT TATTTGCGGA TAAAGAAGAC
    ATTATTTTTG ACATAGAAGA TTACATCTTT ACAACAGAAG CTCATCTGTT ACCTCTTTGG
    CTCTCTACTA CAAATCAAAG CATCTCTAAG AAGAATACAA CTTCAGAATA TACAGAACTG
    GATGACAGTA ATTTTGATTG GACTTGTCCA AACACTCAGA TTCTCTTCCC TAATGACCCA
    TTATATGCTC AGAGCATTCG TGAACCCTTG AAAAATGTGG TGGATAAGAG CTGTCCTAGA
    GGCATCATCA GAGTCAGAGA GGAGAGTTTC AGGAGTGGAG CTAAACCCCC TCTGAGAGCC
    AGAGATTTCA TGGCCACTAA CCCTGAACAT TTAGAAATCT TGAAGAAGAT GGGGGTGAGT
    TTGATTCACC TCAAAGATGG GAGAGTCCAG TTGGTTCAAC ATGCAATCCA AGCTGCTAGT
    TCAATTGATG CTGAGGATGG TTTGAGGTTC ATGCAGGAGA TGATCGAATT GTCAAGTCAG
    CAACAAAAAG AACAGCAGCT GCCTCCACGA GCTGTTCAAA TTGTATCCCA CCCATTTTTT
    GGCAGGGTAG TATTGACTGC TGGACCAGCT CAGTTTGGGC TGGATCTGTC TAAACATAAA
    GAGACAAGAG GATTTGTTGC AAGCAGTAAA CCATACAATG GTTGTTCAGA GCTTACTAAC
    CCCGAGGCAG TGATGGGAAA AATCGCTTTG ATACAAAGAG GACAGTGCAT GTTTGCAGAA
    AAGGCACGCA ACATTCAGAA TGCTGGTGCC ATTGGTGGCA TTGTTATTGA TGACAATGAG
    GGGAGCAGCA GTGATACTGC CCCTCTGTTC CAGATGGCAG GTGATGGAAA AGACACAGAC
    GACATCAAGA TCCCCATGCT ATTCTTATTC AGTAAAGAAG GAAGTATCAT ATTGGATGCC
    ATCCGGGAAT ATGAGGAGGT GGAAGTACTC CTTTCTGACA AAGCAAAAGA TAGAGATCCT
    GAAATGGAAA ATGAAGAACA ACCATCCTCT GAAAATGATT CTCAGAATCA GAGTGCTGAA
    CAGATTACAT CAAGTTCTCA GGAGGTTGAT TTGGTGGATC AAGAGTCTCC TGAGGAAAGT
    TCTCTAAACT CACAACCAGA ATCATCATCT CCAACAGATA CAGACAATGC TGCAAGTGTT
    TCTGCTTCTG AACAGAATTC TAACCCCGTA GAAAACCATG AGACTACAAG TCTTGATGGT
    GAATGTACAG ATTTAGATAA CCAACTTCAA GAACAATCAG AAACCGAAGA AGATTCCAAT
    CCTAATGTTA GCTGGGGTAA AAAGGTCCAG CCTATAGACT CCATATTAGC AGACTGGAAT
    GAAGATATAG AAGCATTTGA AATGATGGAG AAAGACGAAC TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe09159
    Clone ID Related Accession (Same CDS sequence) XM_005622431.3 , XM_005622431.2
    Accession Version XM_005622431.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2805bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005622431.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGCGAAG CCCGCGGCGG CCGGGGCTGT GGGTCCTCGG TCCCCCAGCG AGCGCCATGG 
    AGCCTGGTGG CGGCGGCGGC CGCGCTCTGC CTGGTGGCGG CCACGTCCGT GTGGACGGCG
    GGGGCCGCGC CCATGAGTAG GGAGGAGAGA CAGAAGCTTG GGAATCAAGT ACTGGAAATG
    TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCTGATGA ACTCATGCCT
    TTAACCTGTA GAGGTCGTGT TAGAGGCCAG GAGCCAAGTC GGGGTGATGT TGATGATGCC
    TTGGGAAAAT TTTCCCTGAC ACTGATTGAT TCTTTGGATA CTCTTGTGGT ATTAAATAAA
    ACTAAAGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGAG ATGTTAATTT AGATAATGAT
    GTAGTTGTAT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC
    TCGCTGGCGA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAACTT
    CTCCAAATGG CGAAGCAATT GGGTTACAAA CTTTTACCAG CTTTCAATAC TACCAGTGGC
    CTTCCTTATC CAAGAATTAA TTTAAAATTT GGTATCAGAA AACCGGAAGC TCGAACAGGA
    ACTGAAACAG ATACTTGTAC AGCTTGTGCA GGTACCTTGA TCCTTGAGTT CGCTGCTTTA
    AGTCGATTCA CAGGAGCAAC AATATTTGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC
    TGGGAAAAAA GACAGCGAAG TAGTAATTTA GTGGGTGTGA CTATAAATAT TCATACTGGA
    GACTGGGTAA GAAAAGATAG TGGAGTTGGA GCAGGGATTG ATTCATATTA TGAATATCTG
    TTGAAAGCTT ATGTCTTGCT TGGAGATGAC AGTTTCCTGG AAAGATTTAA TACACACTAT
    GATGCCATAA TGAGGTACAT TAGCCAGCCA CCTCTTCTGC TTGATGTGCA TATCCACAAG
    CCAATGCTGA ATGCTCGGAC TTGGATGGAT GCATTGCTCG CCTTTTTTCC AGGTTTGCAG
    GTCTTAAAGG GGGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA TCAGGTGATT
    AAAAAACATA ACTTCCTACC AGAGGCATTT ACTACAGATT TCAGGGTACA TTGGGCTCAA
    CATCCTTTAA GACCAGAATT TGCAGAAAGT ACCTACTTCT TATATAAAGC TACAGGAGAT
    CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TAAATAAATA TGCTAGAGTG
    CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGTACTGGAA GTCATGAGGA CAGAATGGAT
    TCTTTCTTCT TGGCTGAGAT GTTTAAATAT CTTTACCTAT TATTTGCGGA TAAAGAAGAC
    ATTATTTTTG ACATAGAAGA TTACATCTTT ACAACAGAAG CTCATCTGTT ACCTCTTTGG
    CTCTCTACTA CAAATCAAAG CATCTCTAAG AAGAATACAA CTTCAGAATA TACAGAACTG
    GATGACAGTA ATTTTGATTG GACTTGTCCA AACACTCAGA TTCTCTTCCC TAATGACCCA
    TTATATGCTC AGAGCATTCG TGAACCCTTG AAAAATGTGG TGGATAAGAG CTGTCCTAGA
    GGCATCATCA GAGTCAGAGA GGAGAGTTTC AGGAGTGGAG CTAAACCCCC TCTGAGAGCC
    AGAGATTTCA TGGCCACTAA CCCTGAACAT TTAGAAATCT TGAAGAAGAT GGGGGTGAGT
    TTGATTCACC TCAAAGATGG GAGAGTCCAG TTGGTTCAAC ATGCAATCCA AGCTGCTAGT
    TCAATTGATG CTGAGGATGG TTTGAGGTTC ATGCAGGAGA TGATCGAATT GTCAAGTCAG
    CAACAAAAAG AACAGCAGCT GCCTCCACGA GCTGTTCAAA TTGTATCCCA CCCATTTTTT
    GGCAGGGTAG TATTGACTGC TGGACCAGCT CAGTTTGGGC TGGATCTGTC TAAACATAAA
    GAGACAAGAG GATTTGTTGC AAGCAGTAAA CCATACAATG GTTGTTCAGA GCTTACTAAC
    CCCGAGGCAG TGATGGGAAA AATCGCTTTG ATACAAAGAG GACAGTGCAT GTTTGCAGAA
    AAGGCACGCA ACATTCAGAA TGCTGGTGCC ATTGGTGGCA TTGTTATTGA TGACAATGAG
    GGGAGCAGCA GTGATACTGC CCCTCTGTTC CAGATGGCAG GTGATGGAAA AGACACAGAC
    GACATCAAGA TCCCCATGCT ATTCTTATTC AGTAAAGAAG GAAGTATCAT ATTGGATGCC
    ATCCGGGAAT ATGAGGAGGT GGAAGTACTC CTTTCTGACA AAGCAAAAGA TAGAGATCCT
    GAAATGGAAA ATGAAGAACA ACCATCCTCT GAAAATGATT CTCAGAATCA GAGTGCTGAA
    CAGATTACAT CAAGTTCTCA GGAGGTTGAT TTGGTGGATC AAGAGTCTCC TGAGGAAAGT
    TCTCTAAACT CACAACCAGA ATCATCATCT CCAACAGATA CAGACAATGC TGCAAGTGTT
    TCTGCTTCTG AACAGAATTC TAACCCCGTA GAAAACCATG AGACTACAAG TCTTGATGGT
    GAATGTACAG ATTTAGATAA CCAACTTCAA GAACAATCAG AAACCGAAGA AGATTCCAAT
    CCTAATGTTA GCTGGGGTAA AAAGGTCCAG CCTATAGACT CCATATTAGC AGACTGGAAT
    GAAGATATAG AAGCATTTGA AATGATGGAG AAAGACGAAC TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005622488.1
    CDS224..3028
    Translation

    Target ORF information:

    RefSeq Version XM_005622431.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ER degradation enhancer, mannosidase alpha-like 3 (EDEM3), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005622431.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGCGAAG CCCGCGGCGG CCGGGGCTGT GGGTCCTCGG TCCCCCAGCG AGCGCCATGG 
    AGCCTGGTGG CGGCGGCGGC CGCGCTCTGC CTGGTGGCGG CCACGTCCGT GTGGACGGCG
    GGGGCCGCGC CCATGAGTAG GGAGGAGAGA CAGAAGCTTG GGAATCAAGT ACTGGAAATG
    TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCTGATGA ACTCATGCCT
    TTAACCTGTA GAGGTCGTGT TAGAGGCCAG GAGCCAAGTC GGGGTGATGT TGATGATGCC
    TTGGGAAAAT TTTCCCTGAC ACTGATTGAT TCTTTGGATA CTCTTGTGGT ATTAAATAAA
    ACTAAAGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGAG ATGTTAATTT AGATAATGAT
    GTAGTTGTAT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC
    TCGCTGGCGA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAACTT
    CTCCAAATGG CGAAGCAATT GGGTTACAAA CTTTTACCAG CTTTCAATAC TACCAGTGGC
    CTTCCTTATC CAAGAATTAA TTTAAAATTT GGTATCAGAA AACCGGAAGC TCGAACAGGA
    ACTGAAACAG ATACTTGTAC AGCTTGTGCA GGTACCTTGA TCCTTGAGTT CGCTGCTTTA
    AGTCGATTCA CAGGAGCAAC AATATTTGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC
    TGGGAAAAAA GACAGCGAAG TAGTAATTTA GTGGGTGTGA CTATAAATAT TCATACTGGA
    GACTGGGTAA GAAAAGATAG TGGAGTTGGA GCAGGGATTG ATTCATATTA TGAATATCTG
    TTGAAAGCTT ATGTCTTGCT TGGAGATGAC AGTTTCCTGG AAAGATTTAA TACACACTAT
    GATGCCATAA TGAGGTACAT TAGCCAGCCA CCTCTTCTGC TTGATGTGCA TATCCACAAG
    CCAATGCTGA ATGCTCGGAC TTGGATGGAT GCATTGCTCG CCTTTTTTCC AGGTTTGCAG
    GTCTTAAAGG GGGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA TCAGGTGATT
    AAAAAACATA ACTTCCTACC AGAGGCATTT ACTACAGATT TCAGGGTACA TTGGGCTCAA
    CATCCTTTAA GACCAGAATT TGCAGAAAGT ACCTACTTCT TATATAAAGC TACAGGAGAT
    CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TAAATAAATA TGCTAGAGTG
    CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGTACTGGAA GTCATGAGGA CAGAATGGAT
    TCTTTCTTCT TGGCTGAGAT GTTTAAATAT CTTTACCTAT TATTTGCGGA TAAAGAAGAC
    ATTATTTTTG ACATAGAAGA TTACATCTTT ACAACAGAAG CTCATCTGTT ACCTCTTTGG
    CTCTCTACTA CAAATCAAAG CATCTCTAAG AAGAATACAA CTTCAGAATA TACAGAACTG
    GATGACAGTA ATTTTGATTG GACTTGTCCA AACACTCAGA TTCTCTTCCC TAATGACCCA
    TTATATGCTC AGAGCATTCG TGAACCCTTG AAAAATGTGG TGGATAAGAG CTGTCCTAGA
    GGCATCATCA GAGTCAGAGA GGAGAGTTTC AGGAGTGGAG CTAAACCCCC TCTGAGAGCC
    AGAGATTTCA TGGCCACTAA CCCTGAACAT TTAGAAATCT TGAAGAAGAT GGGGGTGAGT
    TTGATTCACC TCAAAGATGG GAGAGTCCAG TTGGTTCAAC ATGCAATCCA AGCTGCTAGT
    TCAATTGATG CTGAGGATGG TTTGAGGTTC ATGCAGGAGA TGATCGAATT GTCAAGTCAG
    CAACAAAAAG AACAGCAGCT GCCTCCACGA GCTGTTCAAA TTGTATCCCA CCCATTTTTT
    GGCAGGGTAG TATTGACTGC TGGACCAGCT CAGTTTGGGC TGGATCTGTC TAAACATAAA
    GAGACAAGAG GATTTGTTGC AAGCAGTAAA CCATACAATG GTTGTTCAGA GCTTACTAAC
    CCCGAGGCAG TGATGGGAAA AATCGCTTTG ATACAAAGAG GACAGTGCAT GTTTGCAGAA
    AAGGCACGCA ACATTCAGAA TGCTGGTGCC ATTGGTGGCA TTGTTATTGA TGACAATGAG
    GGGAGCAGCA GTGATACTGC CCCTCTGTTC CAGATGGCAG GTGATGGAAA AGACACAGAC
    GACATCAAGA TCCCCATGCT ATTCTTATTC AGTAAAGAAG GAAGTATCAT ATTGGATGCC
    ATCCGGGAAT ATGAGGAGGT GGAAGTACTC CTTTCTGACA AAGCAAAAGA TAGAGATCCT
    GAAATGGAAA ATGAAGAACA ACCATCCTCT GAAAATGATT CTCAGAATCA GAGTGCTGAA
    CAGATTACAT CAAGTTCTCA GGAGGTTGAT TTGGTGGATC AAGAGTCTCC TGAGGAAAGT
    TCTCTAAACT CACAACCAGA ATCATCATCT CCAACAGATA CAGACAATGC TGCAAGTGTT
    TCTGCTTCTG AACAGAATTC TAACCCCGTA GAAAACCATG AGACTACAAG TCTTGATGGT
    GAATGTACAG ATTTAGATAA CCAACTTCAA GAACAATCAG AAACCGAAGA AGATTCCAAT
    CCTAATGTTA GCTGGGGTAA AAAGGTCCAG CCTATAGACT CCATATTAGC AGACTGGAAT
    GAAGATATAG AAGCATTTGA AATGATGGAG AAAGACGAAC TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe09160
    Clone ID Related Accession (Same CDS sequence) XM_537162.6 , XM_537162.5
    Accession Version XM_537162.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2802bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_537162.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGCGAAG CCCGCGGCGG CCGGGGCTGT GGGTCCTCGG TCCCCCAGCG AGCGCCATGG 
    AGCCTGGTGG CGGCGGCGGC CGCGCTCTGC CTGGTGGCGG CCACGTCCGT GTGGACGGCG
    GGGGCCGCGC CCATGAGTAG GGAGGAGAGA CAGAAGCTTG GGAATCAAGT ACTGGAAATG
    TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCTGATGA ACTCATGCCT
    TTAACCTGTA GAGGTCGTGT TAGAGGCCAG GAGCCAAGTC GGGGTGATGT TGATGATGCC
    TTGGGAAAAT TTTCCCTGAC ACTGATTGAT TCTTTGGATA CTCTTGTGGT ATTAAATAAA
    ACTAAAGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGAG ATGTTAATTT AGATAATGAT
    GTAGTTGTAT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC
    TCGCTGGCGA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAACTT
    CTCCAAATGG CGAAGCAATT GGGTTACAAA CTTTTACCAG CTTTCAATAC TACCAGTGGC
    CTTCCTTATC CAAGAATTAA TTTAAAATTT GGTATCAGAA AACCGGAAGC TCGAACAGGA
    ACTGAAACAG ATACTTGTAC AGCTTGTGCA GGTACCTTGA TCCTTGAGTT CGCTGCTTTA
    AGTCGATTCA CAGGAGCAAC AATATTTGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC
    TGGGAAAAAA GACAGCGAAG TAGTAATTTA GTGGGTGTGA CTATAAATAT TCATACTGGA
    GACTGGGTAA GAAAAGATAG TGGAGTTGGA GCAGGGATTG ATTCATATTA TGAATATCTG
    TTGAAAGCTT ATGTCTTGCT TGGAGATGAC AGTTTCCTGG AAAGATTTAA TACACACTAT
    GATGCCATAA TGAGGTACAT TAGCCAGCCA CCTCTTCTGC TTGATGTGCA TATCCACAAG
    CCAATGCTGA ATGCTCGGAC TTGGATGGAT GCATTGCTCG CCTTTTTTCC AGGTTTGCAG
    GTCTTAAAGG GGGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA TCAGGTGATT
    AAAAAACATA ACTTCCTACC AGAGGCATTT ACTACAGATT TCAGGGTACA TTGGGCTCAA
    CATCCTTTAA GACCAGAATT TGCAGAAAGT ACCTACTTCT TATATAAAGC TACAGGAGAT
    CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TAAATAAATA TGCTAGAGTG
    CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGTACTGGAA GTCATGAGGA CAGAATGGAT
    TCTTTCTTCT TGGCTGAGAT GTTTAAATAT CTTTACCTAT TATTTGCGGA TAAAGAAGAC
    ATTATTTTTG ACATAGAAGA TTACATCTTT ACAACAGAAG CTCATCTGTT ACCTCTTTGG
    CTCTCTACTA CAAATCAAAG CATCTCTAAG AAGAATACAA CTTCAGAATA TACAGAACTG
    GATGACAGTA ATTTTGATTG GACTTGTCCA AACACTCAGA TTCTCTTCCC TAATGACCCA
    TTATATGCTC AGAGCATTCG TGAACCCTTG AAAAATGTGG TGGATAAGAG CTGTCCTAGA
    GGCATCATCA GAGTAGAGGA GAGTTTCAGG AGTGGAGCTA AACCCCCTCT GAGAGCCAGA
    GATTTCATGG CCACTAACCC TGAACATTTA GAAATCTTGA AGAAGATGGG GGTGAGTTTG
    ATTCACCTCA AAGATGGGAG AGTCCAGTTG GTTCAACATG CAATCCAAGC TGCTAGTTCA
    ATTGATGCTG AGGATGGTTT GAGGTTCATG CAGGAGATGA TCGAATTGTC AAGTCAGCAA
    CAAAAAGAAC AGCAGCTGCC TCCACGAGCT GTTCAAATTG TATCCCACCC ATTTTTTGGC
    AGGGTAGTAT TGACTGCTGG ACCAGCTCAG TTTGGGCTGG ATCTGTCTAA ACATAAAGAG
    ACAAGAGGAT TTGTTGCAAG CAGTAAACCA TACAATGGTT GTTCAGAGCT TACTAACCCC
    GAGGCAGTGA TGGGAAAAAT CGCTTTGATA CAAAGAGGAC AGTGCATGTT TGCAGAAAAG
    GCACGCAACA TTCAGAATGC TGGTGCCATT GGTGGCATTG TTATTGATGA CAATGAGGGG
    AGCAGCAGTG ATACTGCCCC TCTGTTCCAG ATGGCAGGTG ATGGAAAAGA CACAGACGAC
    ATCAAGATCC CCATGCTATT CTTATTCAGT AAAGAAGGAA GTATCATATT GGATGCCATC
    CGGGAATATG AGGAGGTGGA AGTACTCCTT TCTGACAAAG CAAAAGATAG AGATCCTGAA
    ATGGAAAATG AAGAACAACC ATCCTCTGAA AATGATTCTC AGAATCAGAG TGCTGAACAG
    ATTACATCAA GTTCTCAGGA GGTTGATTTG GTGGATCAAG AGTCTCCTGA GGAAAGTTCT
    CTAAACTCAC AACCAGAATC ATCATCTCCA ACAGATACAG ACAATGCTGC AAGTGTTTCT
    GCTTCTGAAC AGAATTCTAA CCCCGTAGAA AACCATGAGA CTACAAGTCT TGATGGTGAA
    TGTACAGATT TAGATAACCA ACTTCAAGAA CAATCAGAAA CCGAAGAAGA TTCCAATCCT
    AATGTTAGCT GGGGTAAAAA GGTCCAGCCT ATAGACTCCA TATTAGCAGA CTGGAATGAA
    GATATAGAAG CATTTGAAAT GATGGAGAAA GACGAACTAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_537162.2
    CDS219..3020
    Translation

    Target ORF information:

    RefSeq Version XM_537162.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ER degradation enhancing alpha-mannosidase like protein 3 (EDEM3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_537162.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGCGAAG CCCGCGGCGG CCGGGGCTGT GGGTCCTCGG TCCCCCAGCG AGCGCCATGG 
    AGCCTGGTGG CGGCGGCGGC CGCGCTCTGC CTGGTGGCGG CCACGTCCGT GTGGACGGCG
    GGGGCCGCGC CCATGAGTAG GGAGGAGAGA CAGAAGCTTG GGAATCAAGT ACTGGAAATG
    TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCTGATGA ACTCATGCCT
    TTAACCTGTA GAGGTCGTGT TAGAGGCCAG GAGCCAAGTC GGGGTGATGT TGATGATGCC
    TTGGGAAAAT TTTCCCTGAC ACTGATTGAT TCTTTGGATA CTCTTGTGGT ATTAAATAAA
    ACTAAAGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGAG ATGTTAATTT AGATAATGAT
    GTAGTTGTAT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC
    TCGCTGGCGA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAACTT
    CTCCAAATGG CGAAGCAATT GGGTTACAAA CTTTTACCAG CTTTCAATAC TACCAGTGGC
    CTTCCTTATC CAAGAATTAA TTTAAAATTT GGTATCAGAA AACCGGAAGC TCGAACAGGA
    ACTGAAACAG ATACTTGTAC AGCTTGTGCA GGTACCTTGA TCCTTGAGTT CGCTGCTTTA
    AGTCGATTCA CAGGAGCAAC AATATTTGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC
    TGGGAAAAAA GACAGCGAAG TAGTAATTTA GTGGGTGTGA CTATAAATAT TCATACTGGA
    GACTGGGTAA GAAAAGATAG TGGAGTTGGA GCAGGGATTG ATTCATATTA TGAATATCTG
    TTGAAAGCTT ATGTCTTGCT TGGAGATGAC AGTTTCCTGG AAAGATTTAA TACACACTAT
    GATGCCATAA TGAGGTACAT TAGCCAGCCA CCTCTTCTGC TTGATGTGCA TATCCACAAG
    CCAATGCTGA ATGCTCGGAC TTGGATGGAT GCATTGCTCG CCTTTTTTCC AGGTTTGCAG
    GTCTTAAAGG GGGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA TCAGGTGATT
    AAAAAACATA ACTTCCTACC AGAGGCATTT ACTACAGATT TCAGGGTACA TTGGGCTCAA
    CATCCTTTAA GACCAGAATT TGCAGAAAGT ACCTACTTCT TATATAAAGC TACAGGAGAT
    CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TAAATAAATA TGCTAGAGTG
    CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGTACTGGAA GTCATGAGGA CAGAATGGAT
    TCTTTCTTCT TGGCTGAGAT GTTTAAATAT CTTTACCTAT TATTTGCGGA TAAAGAAGAC
    ATTATTTTTG ACATAGAAGA TTACATCTTT ACAACAGAAG CTCATCTGTT ACCTCTTTGG
    CTCTCTACTA CAAATCAAAG CATCTCTAAG AAGAATACAA CTTCAGAATA TACAGAACTG
    GATGACAGTA ATTTTGATTG GACTTGTCCA AACACTCAGA TTCTCTTCCC TAATGACCCA
    TTATATGCTC AGAGCATTCG TGAACCCTTG AAAAATGTGG TGGATAAGAG CTGTCCTAGA
    GGCATCATCA GAGTAGAGGA GAGTTTCAGG AGTGGAGCTA AACCCCCTCT GAGAGCCAGA
    GATTTCATGG CCACTAACCC TGAACATTTA GAAATCTTGA AGAAGATGGG GGTGAGTTTG
    ATTCACCTCA AAGATGGGAG AGTCCAGTTG GTTCAACATG CAATCCAAGC TGCTAGTTCA
    ATTGATGCTG AGGATGGTTT GAGGTTCATG CAGGAGATGA TCGAATTGTC AAGTCAGCAA
    CAAAAAGAAC AGCAGCTGCC TCCACGAGCT GTTCAAATTG TATCCCACCC ATTTTTTGGC
    AGGGTAGTAT TGACTGCTGG ACCAGCTCAG TTTGGGCTGG ATCTGTCTAA ACATAAAGAG
    ACAAGAGGAT TTGTTGCAAG CAGTAAACCA TACAATGGTT GTTCAGAGCT TACTAACCCC
    GAGGCAGTGA TGGGAAAAAT CGCTTTGATA CAAAGAGGAC AGTGCATGTT TGCAGAAAAG
    GCACGCAACA TTCAGAATGC TGGTGCCATT GGTGGCATTG TTATTGATGA CAATGAGGGG
    AGCAGCAGTG ATACTGCCCC TCTGTTCCAG ATGGCAGGTG ATGGAAAAGA CACAGACGAC
    ATCAAGATCC CCATGCTATT CTTATTCAGT AAAGAAGGAA GTATCATATT GGATGCCATC
    CGGGAATATG AGGAGGTGGA AGTACTCCTT TCTGACAAAG CAAAAGATAG AGATCCTGAA
    ATGGAAAATG AAGAACAACC ATCCTCTGAA AATGATTCTC AGAATCAGAG TGCTGAACAG
    ATTACATCAA GTTCTCAGGA GGTTGATTTG GTGGATCAAG AGTCTCCTGA GGAAAGTTCT
    CTAAACTCAC AACCAGAATC ATCATCTCCA ACAGATACAG ACAATGCTGC AAGTGTTTCT
    GCTTCTGAAC AGAATTCTAA CCCCGTAGAA AACCATGAGA CTACAAGTCT TGATGGTGAA
    TGTACAGATT TAGATAACCA ACTTCAAGAA CAATCAGAAA CCGAAGAAGA TTCCAATCCT
    AATGTTAGCT GGGGTAAAAA GGTCCAGCCT ATAGACTCCA TATTAGCAGA CTGGAATGAA
    GATATAGAAG CATTTGAAAT GATGGAGAAA GACGAACTAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe09160
    Clone ID Related Accession (Same CDS sequence) XM_537162.6 , XM_537162.5
    Accession Version XM_537162.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2802bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_537162.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGCGAAG CCCGCGGCGG CCGGGGCTGT GGGTCCTCGG TCCCCCAGCG AGCGCCATGG 
    AGCCTGGTGG CGGCGGCGGC CGCGCTCTGC CTGGTGGCGG CCACGTCCGT GTGGACGGCG
    GGGGCCGCGC CCATGAGTAG GGAGGAGAGA CAGAAGCTTG GGAATCAAGT ACTGGAAATG
    TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCTGATGA ACTCATGCCT
    TTAACCTGTA GAGGTCGTGT TAGAGGCCAG GAGCCAAGTC GGGGTGATGT TGATGATGCC
    TTGGGAAAAT TTTCCCTGAC ACTGATTGAT TCTTTGGATA CTCTTGTGGT ATTAAATAAA
    ACTAAAGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGAG ATGTTAATTT AGATAATGAT
    GTAGTTGTAT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC
    TCGCTGGCGA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAACTT
    CTCCAAATGG CGAAGCAATT GGGTTACAAA CTTTTACCAG CTTTCAATAC TACCAGTGGC
    CTTCCTTATC CAAGAATTAA TTTAAAATTT GGTATCAGAA AACCGGAAGC TCGAACAGGA
    ACTGAAACAG ATACTTGTAC AGCTTGTGCA GGTACCTTGA TCCTTGAGTT CGCTGCTTTA
    AGTCGATTCA CAGGAGCAAC AATATTTGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC
    TGGGAAAAAA GACAGCGAAG TAGTAATTTA GTGGGTGTGA CTATAAATAT TCATACTGGA
    GACTGGGTAA GAAAAGATAG TGGAGTTGGA GCAGGGATTG ATTCATATTA TGAATATCTG
    TTGAAAGCTT ATGTCTTGCT TGGAGATGAC AGTTTCCTGG AAAGATTTAA TACACACTAT
    GATGCCATAA TGAGGTACAT TAGCCAGCCA CCTCTTCTGC TTGATGTGCA TATCCACAAG
    CCAATGCTGA ATGCTCGGAC TTGGATGGAT GCATTGCTCG CCTTTTTTCC AGGTTTGCAG
    GTCTTAAAGG GGGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA TCAGGTGATT
    AAAAAACATA ACTTCCTACC AGAGGCATTT ACTACAGATT TCAGGGTACA TTGGGCTCAA
    CATCCTTTAA GACCAGAATT TGCAGAAAGT ACCTACTTCT TATATAAAGC TACAGGAGAT
    CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TAAATAAATA TGCTAGAGTG
    CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGTACTGGAA GTCATGAGGA CAGAATGGAT
    TCTTTCTTCT TGGCTGAGAT GTTTAAATAT CTTTACCTAT TATTTGCGGA TAAAGAAGAC
    ATTATTTTTG ACATAGAAGA TTACATCTTT ACAACAGAAG CTCATCTGTT ACCTCTTTGG
    CTCTCTACTA CAAATCAAAG CATCTCTAAG AAGAATACAA CTTCAGAATA TACAGAACTG
    GATGACAGTA ATTTTGATTG GACTTGTCCA AACACTCAGA TTCTCTTCCC TAATGACCCA
    TTATATGCTC AGAGCATTCG TGAACCCTTG AAAAATGTGG TGGATAAGAG CTGTCCTAGA
    GGCATCATCA GAGTAGAGGA GAGTTTCAGG AGTGGAGCTA AACCCCCTCT GAGAGCCAGA
    GATTTCATGG CCACTAACCC TGAACATTTA GAAATCTTGA AGAAGATGGG GGTGAGTTTG
    ATTCACCTCA AAGATGGGAG AGTCCAGTTG GTTCAACATG CAATCCAAGC TGCTAGTTCA
    ATTGATGCTG AGGATGGTTT GAGGTTCATG CAGGAGATGA TCGAATTGTC AAGTCAGCAA
    CAAAAAGAAC AGCAGCTGCC TCCACGAGCT GTTCAAATTG TATCCCACCC ATTTTTTGGC
    AGGGTAGTAT TGACTGCTGG ACCAGCTCAG TTTGGGCTGG ATCTGTCTAA ACATAAAGAG
    ACAAGAGGAT TTGTTGCAAG CAGTAAACCA TACAATGGTT GTTCAGAGCT TACTAACCCC
    GAGGCAGTGA TGGGAAAAAT CGCTTTGATA CAAAGAGGAC AGTGCATGTT TGCAGAAAAG
    GCACGCAACA TTCAGAATGC TGGTGCCATT GGTGGCATTG TTATTGATGA CAATGAGGGG
    AGCAGCAGTG ATACTGCCCC TCTGTTCCAG ATGGCAGGTG ATGGAAAAGA CACAGACGAC
    ATCAAGATCC CCATGCTATT CTTATTCAGT AAAGAAGGAA GTATCATATT GGATGCCATC
    CGGGAATATG AGGAGGTGGA AGTACTCCTT TCTGACAAAG CAAAAGATAG AGATCCTGAA
    ATGGAAAATG AAGAACAACC ATCCTCTGAA AATGATTCTC AGAATCAGAG TGCTGAACAG
    ATTACATCAA GTTCTCAGGA GGTTGATTTG GTGGATCAAG AGTCTCCTGA GGAAAGTTCT
    CTAAACTCAC AACCAGAATC ATCATCTCCA ACAGATACAG ACAATGCTGC AAGTGTTTCT
    GCTTCTGAAC AGAATTCTAA CCCCGTAGAA AACCATGAGA CTACAAGTCT TGATGGTGAA
    TGTACAGATT TAGATAACCA ACTTCAAGAA CAATCAGAAA CCGAAGAAGA TTCCAATCCT
    AATGTTAGCT GGGGTAAAAA GGTCCAGCCT ATAGACTCCA TATTAGCAGA CTGGAATGAA
    GATATAGAAG CATTTGAAAT GATGGAGAAA GACGAACTAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_537162.2
    CDS224..3025
    Translation

    Target ORF information:

    RefSeq Version XM_537162.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ER degradation enhancer, mannosidase alpha-like 3 (EDEM3), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_537162.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGCGAAG CCCGCGGCGG CCGGGGCTGT GGGTCCTCGG TCCCCCAGCG AGCGCCATGG 
    AGCCTGGTGG CGGCGGCGGC CGCGCTCTGC CTGGTGGCGG CCACGTCCGT GTGGACGGCG
    GGGGCCGCGC CCATGAGTAG GGAGGAGAGA CAGAAGCTTG GGAATCAAGT ACTGGAAATG
    TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCTGATGA ACTCATGCCT
    TTAACCTGTA GAGGTCGTGT TAGAGGCCAG GAGCCAAGTC GGGGTGATGT TGATGATGCC
    TTGGGAAAAT TTTCCCTGAC ACTGATTGAT TCTTTGGATA CTCTTGTGGT ATTAAATAAA
    ACTAAAGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGAG ATGTTAATTT AGATAATGAT
    GTAGTTGTAT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC
    TCGCTGGCGA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAACTT
    CTCCAAATGG CGAAGCAATT GGGTTACAAA CTTTTACCAG CTTTCAATAC TACCAGTGGC
    CTTCCTTATC CAAGAATTAA TTTAAAATTT GGTATCAGAA AACCGGAAGC TCGAACAGGA
    ACTGAAACAG ATACTTGTAC AGCTTGTGCA GGTACCTTGA TCCTTGAGTT CGCTGCTTTA
    AGTCGATTCA CAGGAGCAAC AATATTTGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC
    TGGGAAAAAA GACAGCGAAG TAGTAATTTA GTGGGTGTGA CTATAAATAT TCATACTGGA
    GACTGGGTAA GAAAAGATAG TGGAGTTGGA GCAGGGATTG ATTCATATTA TGAATATCTG
    TTGAAAGCTT ATGTCTTGCT TGGAGATGAC AGTTTCCTGG AAAGATTTAA TACACACTAT
    GATGCCATAA TGAGGTACAT TAGCCAGCCA CCTCTTCTGC TTGATGTGCA TATCCACAAG
    CCAATGCTGA ATGCTCGGAC TTGGATGGAT GCATTGCTCG CCTTTTTTCC AGGTTTGCAG
    GTCTTAAAGG GGGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA TCAGGTGATT
    AAAAAACATA ACTTCCTACC AGAGGCATTT ACTACAGATT TCAGGGTACA TTGGGCTCAA
    CATCCTTTAA GACCAGAATT TGCAGAAAGT ACCTACTTCT TATATAAAGC TACAGGAGAT
    CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TAAATAAATA TGCTAGAGTG
    CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGTACTGGAA GTCATGAGGA CAGAATGGAT
    TCTTTCTTCT TGGCTGAGAT GTTTAAATAT CTTTACCTAT TATTTGCGGA TAAAGAAGAC
    ATTATTTTTG ACATAGAAGA TTACATCTTT ACAACAGAAG CTCATCTGTT ACCTCTTTGG
    CTCTCTACTA CAAATCAAAG CATCTCTAAG AAGAATACAA CTTCAGAATA TACAGAACTG
    GATGACAGTA ATTTTGATTG GACTTGTCCA AACACTCAGA TTCTCTTCCC TAATGACCCA
    TTATATGCTC AGAGCATTCG TGAACCCTTG AAAAATGTGG TGGATAAGAG CTGTCCTAGA
    GGCATCATCA GAGTAGAGGA GAGTTTCAGG AGTGGAGCTA AACCCCCTCT GAGAGCCAGA
    GATTTCATGG CCACTAACCC TGAACATTTA GAAATCTTGA AGAAGATGGG GGTGAGTTTG
    ATTCACCTCA AAGATGGGAG AGTCCAGTTG GTTCAACATG CAATCCAAGC TGCTAGTTCA
    ATTGATGCTG AGGATGGTTT GAGGTTCATG CAGGAGATGA TCGAATTGTC AAGTCAGCAA
    CAAAAAGAAC AGCAGCTGCC TCCACGAGCT GTTCAAATTG TATCCCACCC ATTTTTTGGC
    AGGGTAGTAT TGACTGCTGG ACCAGCTCAG TTTGGGCTGG ATCTGTCTAA ACATAAAGAG
    ACAAGAGGAT TTGTTGCAAG CAGTAAACCA TACAATGGTT GTTCAGAGCT TACTAACCCC
    GAGGCAGTGA TGGGAAAAAT CGCTTTGATA CAAAGAGGAC AGTGCATGTT TGCAGAAAAG
    GCACGCAACA TTCAGAATGC TGGTGCCATT GGTGGCATTG TTATTGATGA CAATGAGGGG
    AGCAGCAGTG ATACTGCCCC TCTGTTCCAG ATGGCAGGTG ATGGAAAAGA CACAGACGAC
    ATCAAGATCC CCATGCTATT CTTATTCAGT AAAGAAGGAA GTATCATATT GGATGCCATC
    CGGGAATATG AGGAGGTGGA AGTACTCCTT TCTGACAAAG CAAAAGATAG AGATCCTGAA
    ATGGAAAATG AAGAACAACC ATCCTCTGAA AATGATTCTC AGAATCAGAG TGCTGAACAG
    ATTACATCAA GTTCTCAGGA GGTTGATTTG GTGGATCAAG AGTCTCCTGA GGAAAGTTCT
    CTAAACTCAC AACCAGAATC ATCATCTCCA ACAGATACAG ACAATGCTGC AAGTGTTTCT
    GCTTCTGAAC AGAATTCTAA CCCCGTAGAA AACCATGAGA CTACAAGTCT TGATGGTGAA
    TGTACAGATT TAGATAACCA ACTTCAAGAA CAATCAGAAA CCGAAGAAGA TTCCAATCCT
    AATGTTAGCT GGGGTAAAAA GGTCCAGCCT ATAGACTCCA TATTAGCAGA CTGGAATGAA
    GATATAGAAG CATTTGAAAT GATGGAGAAA GACGAACTAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe37426
    Clone ID Related Accession (Same CDS sequence) XM_014115137.1
    Accession Version XM_014115137.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2850bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGCGAAG CCCGCGGCGG CCGGGGCTGT GGGTCCTCGG TCCCCCAGCG AGCGCCATGG 
    AGCCTGGTGG CGGCGGCGGC CGCGCTCTGC CTGGTGGCGG CCACGTCCGT GTGGACGGCG
    GGGGCCGCGC CCATGAGTAG GGAGGAGAGA CAGAAGCTTG GGAATCAAGT ACTGGAAATG
    TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCTGATGA ACTCATGCCT
    TTAACCTGTA GAGGTCGTGT TAGAGGCCAG GAGCCAAGTC GGGGTGATGT TGATGATGCC
    TTGGGAAAAT TTTCCCTGAC ACTGATTGAT TCTTTGGATA CTCTTGTGGT ATTAAATAAA
    ACTAAAGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGAG ATGTTAATTT AGATAATGAT
    GTAGTTGTAT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC
    TCGCTGGCGA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAACTT
    CTCCAAATGG CGAAGCAATT GGGTTACAAA CTTTTACCAG CTTTCAATAC TACCAGTGGC
    CTTCCTTATC CAAGAATTAA TTTAAAATTT GGTATCAGAA AACCGGAAGC TCGAACAGGA
    ACTGAAACAG ATACTTGTAC AGCTTGTGCA GGTACCTTGA TCCTTGAGTT CGCTGCTTTA
    AGTCGATTCA CAGGAGCAAC AATATTTGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC
    TGGGAAAAAA GACAGCGAAG TAGTAATTTA GTGGGTGTGA CTATAAATAT TCATACTGGA
    GACTGGGTAA GAAAAGATAG TGGAGTTGGA GCAGGGATTG ATTCATATTA TGAATATCTG
    TTGAAAGCTT ATGTCTTGCT TGGAGATGAC AGTTTCCTGG AAAGATTTAA TACACACTAT
    GATGCCATAA TGAGGTACAT TAGCCAGCCA CCTCTTCTGC TTGATGTGCA TATCCACAAG
    CCAATGCTGA ATGCTCGGAC TTGGATGGAT GCATTGCTCG CCTTTTTTCC AGGTTTGCAG
    GTCTTAAAGG GGGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA TCAGGTGATT
    AAAAAACATA ACTTCCTACC AGAGGCATTT ACTACAGATT TCAGGGTACA TTGGGCTCAA
    CATCCTTTAA GACCAGAATT TGCAGAAAGT ACCTACTTCT TATATAAAGC TACAGGAGAT
    CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TAAATAAATA TGCTAGAGTG
    CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGTACTGGAA GTCATGAGGA CAGAATGGAT
    TCTTTCTTCT TGGCTGAGAT GTTTAAATAT CTTTACCTAT TATTTGCGGA TAAAGAAGAC
    ATTATTTTTG ACATAGAAGA TTACATCTTT ACAACAGAAG CTCATCTGTT ACCTCTTTGG
    CTCTCTACTA CAAATCAAAG CATCTCTAAG AAGAATACAA CTTCAGAATA TACAGAACTG
    GATGACAGTA ATTTTGATTG GACTTGTCCA AACACTCAGA TTCTCTTCCC TAATGACCCA
    TTATATGCTC AGAGCATTCG TGAACCCTTG AAAAATGTGG TGGATAAGAG CTGTCCTAGA
    GGCATCATCA GAGTCAGAGA GGAGAGTTTC AGGAGTGGAG CTAAACCCCC TCTGAGAGCC
    AGAGATTTCA TGGCCACTAA CCCTGAACAT TTAGAAATCT TGAAGAAGAT GGGGGTGAGT
    TTGATTCACC TCAAAGATGG GAGAGTCCAG TTGGTTCAAC ATGCAATCCA AGCTGCTAGT
    TCAATTGATG CTGAGGATGG TTTGAGGTTC ATGCAGGAGA TGATCGAATT GTCAAGTCAG
    CAACAAAAAG AACAGCAGCT GCCTCCACGA GCTGTTCAAA TTGTATCCCA CCCATTTTTT
    GGCAGGGTAG TATTGACTGC TGGACCAGCT CAGTTTGGGC TGGATCTGTC TAAACATAAA
    GAGACAAGAG GATTTGTTGC AAGCAGTAAA CCATACAATG GTTGTTCAGA GCTTACTAAC
    CCCGAGGCAG TGATGGGAAA AATCGCTTTG ATACAAAGAG GACAGTGCAT GTTTGCAGAA
    AAGGCACGCA ACATTCAGAA TGCTGGTGCC ATTGGTGGCA TTGTTATTGA TGACAATGAG
    GGGAGCAGCA GTGATACTGC CCCTCTGTTC CAGATGGCAG GTGATGGAAA AGACACAGAC
    GACATCAAGA TCCCCATGCT ATTCTTATTC AGTAAAGAAG GAAGTATCAT ATTGGATGCC
    ATCCGGGAAT ATGAGGAGGT GGAAGTACTC CTTTCTGACA AAGCAAAAGA TAGAGCAATA
    TTAAAAGGTA AAATGATTCC AAGCTACATT ATTAATAGTA ATCCTGAAAT GGAAAATGAA
    GAACAACCAT CCTCTGAAAA TGATTCTCAG AATCAGAGTG CTGAACAGAT TACATCAAGT
    TCTCAGGAGG TTGATTTGGT GGATCAAGAG TCTCCTGAGG AAAGTTCTCT AAACTCACAA
    CCAGAATCAT CATCTCCAAC AGATACAGAC AATGCTGCAA GTGTTTCTGC TTCTGAACAG
    AATTCTAACC CCGTAGAAAA CCATGAGACT ACAAGTCTTG ATGGTGAATG TACAGATTTA
    GATAACCAAC TTCAAGAACA ATCAGAAACC GAAGAAGATT CCAATCCTAA TGTTAGCTGG
    GGTAAAAAGG TCCAGCCTAT AGACTCCATA TTAGCAGACT GGAATGAAGA TATAGAAGCA
    TTTGAAATGA TGGAGAAAGA CGAACTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013970612.1
    CDS224..3073
    Translation

    Target ORF information:

    RefSeq Version XM_014115137.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ER degradation enhancer, mannosidase alpha-like 3 (EDEM3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014115137.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGCGAAG CCCGCGGCGG CCGGGGCTGT GGGTCCTCGG TCCCCCAGCG AGCGCCATGG 
    AGCCTGGTGG CGGCGGCGGC CGCGCTCTGC CTGGTGGCGG CCACGTCCGT GTGGACGGCG
    GGGGCCGCGC CCATGAGTAG GGAGGAGAGA CAGAAGCTTG GGAATCAAGT ACTGGAAATG
    TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCTGATGA ACTCATGCCT
    TTAACCTGTA GAGGTCGTGT TAGAGGCCAG GAGCCAAGTC GGGGTGATGT TGATGATGCC
    TTGGGAAAAT TTTCCCTGAC ACTGATTGAT TCTTTGGATA CTCTTGTGGT ATTAAATAAA
    ACTAAAGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGAG ATGTTAATTT AGATAATGAT
    GTAGTTGTAT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC
    TCGCTGGCGA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAACTT
    CTCCAAATGG CGAAGCAATT GGGTTACAAA CTTTTACCAG CTTTCAATAC TACCAGTGGC
    CTTCCTTATC CAAGAATTAA TTTAAAATTT GGTATCAGAA AACCGGAAGC TCGAACAGGA
    ACTGAAACAG ATACTTGTAC AGCTTGTGCA GGTACCTTGA TCCTTGAGTT CGCTGCTTTA
    AGTCGATTCA CAGGAGCAAC AATATTTGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC
    TGGGAAAAAA GACAGCGAAG TAGTAATTTA GTGGGTGTGA CTATAAATAT TCATACTGGA
    GACTGGGTAA GAAAAGATAG TGGAGTTGGA GCAGGGATTG ATTCATATTA TGAATATCTG
    TTGAAAGCTT ATGTCTTGCT TGGAGATGAC AGTTTCCTGG AAAGATTTAA TACACACTAT
    GATGCCATAA TGAGGTACAT TAGCCAGCCA CCTCTTCTGC TTGATGTGCA TATCCACAAG
    CCAATGCTGA ATGCTCGGAC TTGGATGGAT GCATTGCTCG CCTTTTTTCC AGGTTTGCAG
    GTCTTAAAGG GGGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA TCAGGTGATT
    AAAAAACATA ACTTCCTACC AGAGGCATTT ACTACAGATT TCAGGGTACA TTGGGCTCAA
    CATCCTTTAA GACCAGAATT TGCAGAAAGT ACCTACTTCT TATATAAAGC TACAGGAGAT
    CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TAAATAAATA TGCTAGAGTG
    CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGTACTGGAA GTCATGAGGA CAGAATGGAT
    TCTTTCTTCT TGGCTGAGAT GTTTAAATAT CTTTACCTAT TATTTGCGGA TAAAGAAGAC
    ATTATTTTTG ACATAGAAGA TTACATCTTT ACAACAGAAG CTCATCTGTT ACCTCTTTGG
    CTCTCTACTA CAAATCAAAG CATCTCTAAG AAGAATACAA CTTCAGAATA TACAGAACTG
    GATGACAGTA ATTTTGATTG GACTTGTCCA AACACTCAGA TTCTCTTCCC TAATGACCCA
    TTATATGCTC AGAGCATTCG TGAACCCTTG AAAAATGTGG TGGATAAGAG CTGTCCTAGA
    GGCATCATCA GAGTCAGAGA GGAGAGTTTC AGGAGTGGAG CTAAACCCCC TCTGAGAGCC
    AGAGATTTCA TGGCCACTAA CCCTGAACAT TTAGAAATCT TGAAGAAGAT GGGGGTGAGT
    TTGATTCACC TCAAAGATGG GAGAGTCCAG TTGGTTCAAC ATGCAATCCA AGCTGCTAGT
    TCAATTGATG CTGAGGATGG TTTGAGGTTC ATGCAGGAGA TGATCGAATT GTCAAGTCAG
    CAACAAAAAG AACAGCAGCT GCCTCCACGA GCTGTTCAAA TTGTATCCCA CCCATTTTTT
    GGCAGGGTAG TATTGACTGC TGGACCAGCT CAGTTTGGGC TGGATCTGTC TAAACATAAA
    GAGACAAGAG GATTTGTTGC AAGCAGTAAA CCATACAATG GTTGTTCAGA GCTTACTAAC
    CCCGAGGCAG TGATGGGAAA AATCGCTTTG ATACAAAGAG GACAGTGCAT GTTTGCAGAA
    AAGGCACGCA ACATTCAGAA TGCTGGTGCC ATTGGTGGCA TTGTTATTGA TGACAATGAG
    GGGAGCAGCA GTGATACTGC CCCTCTGTTC CAGATGGCAG GTGATGGAAA AGACACAGAC
    GACATCAAGA TCCCCATGCT ATTCTTATTC AGTAAAGAAG GAAGTATCAT ATTGGATGCC
    ATCCGGGAAT ATGAGGAGGT GGAAGTACTC CTTTCTGACA AAGCAAAAGA TAGAGCAATA
    TTAAAAGGTA AAATGATTCC AAGCTACATT ATTAATAGTA ATCCTGAAAT GGAAAATGAA
    GAACAACCAT CCTCTGAAAA TGATTCTCAG AATCAGAGTG CTGAACAGAT TACATCAAGT
    TCTCAGGAGG TTGATTTGGT GGATCAAGAG TCTCCTGAGG AAAGTTCTCT AAACTCACAA
    CCAGAATCAT CATCTCCAAC AGATACAGAC AATGCTGCAA GTGTTTCTGC TTCTGAACAG
    AATTCTAACC CCGTAGAAAA CCATGAGACT ACAAGTCTTG ATGGTGAATG TACAGATTTA
    GATAACCAAC TTCAAGAACA ATCAGAAACC GAAGAAGATT CCAATCCTAA TGTTAGCTGG
    GGTAAAAAGG TCCAGCCTAT AGACTCCATA TTAGCAGACT GGAATGAAGA TATAGAAGCA
    TTTGAAATGA TGGAGAAAGA CGAACTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe37427
    Clone ID Related Accession (Same CDS sequence) XM_014115138.1
    Accession Version XM_014115138.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2820bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGCGAAG CCCGCGGCGG CCGGGGCTGT GGGTCCTCGG TCCCCCAGCG AGCGCCATGG 
    AGCCTGGTGG CGGCGGCGGC CGCGCTCTGC CTGGTGGCGG CCACGTCCGT GTGGACGGCG
    GGGGCCGCGC CCATGAGTAG GGAGGAGAGA CAGAAGCTTG GGAATCAAGT ACTGGAAATG
    TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCTGATGA ACTCATGCCT
    TTAACCTGTA GAGGTCGTGT TAGAGGCCAG GAGCCAAGTC GGGGTGATGT TGATGATGCC
    TTGGGAAAAT TTTCCCTGAC ACTGATTGAT TCTTTGGATA CTCTTGTGGT ATTAAATAAA
    ACTAAAGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGAG ATGTTAATTT AGATAATGAT
    GTAGTTGTAT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC
    TCGCTGGCGA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAACTT
    CTCCAAATGG CGAAGCAATT GGGTTACAAA CTTTTACCAG CTTTCAATAC TACCAGTGGC
    CTTCCTTATC CAAGAATTAA TTTAAAATTT GGTATCAGAA AACCGGAAGC TCGAACAGGA
    ACTGAAACAG ATACTTGTAC AGCTTGTGCA GGTACCTTGA TCCTTGAGTT CGCTGCTTTA
    AGTCGATTCA CAGGAGCAAC AATATTTGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC
    TGGGAAAAAA GACAGCGAAG TAGTAATTTA GTGGGTGTGA CTATAAATAT TCATACTGGA
    GACTGGGTAA GAAAAGATAG TGGAGTTGGA GCAGGGATTG ATTCATATTA TGAATATCTG
    TTGAAAGCTT ATGTCTTGCT TGGAGATGAC AGTTTCCTGG AAAGATTTAA TACACACTAT
    GATGCCATAA TGAGGTACAT TAGCCAGCCA CCTCTTCTGC TTGATGTGCA TATCCACAAG
    CCAATGCTGA ATGCTCGGAC TTGGATGGAT GCATTGCTCG CCTTTTTTCC AGGTTTGCAG
    GTCTTAAAGG GGGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA TCAGGTGATT
    AAAAAACATA ACTTCCTACC AGAGGCATTT ACTACAGATT TCAGGGTACA TTGGGCTCAA
    CATCCTTTAA GACCAGAATT TGCAGAAAGT ACCTACTTCT TATATAAAGC TACAGGAGAT
    CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TAAATAAATA TGCTAGAGTG
    CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGTACTGGAA GTCATGAGGA CAGAATGGAT
    TCTTTCTTCT TGGCTGAGAT GTTTAAATAT CTTTACCTAT TATTTGCGGA TAAAGAAGAC
    ATTATTTTTG ACATAGAAGA TTACATCTTT ACAACAGAAG CTCATCTGTT ACCTCTTTGG
    CTCTCTACTA CAAATCAAAG CATCTCTAAG AAGAATACAA CTTCAGAATA TACAGAACTG
    GATGACAGTA ATTTTGATTG GACTTGTCCA AACACTCAGA TTCTCTTCCC TAATGACCCA
    TTATATGCTC AGAGCATTCG TGAACCCTTG AAAAATGTGG TGGATAAGAG CTGTCCTAGA
    GGCATCATCA GAGTCAGAGA GGAGAGTTTC AGGAGTGGAG CTAAACCCCC TCTGAGAGCC
    AGAGATTTCA TGGCCACTAA CCCTGAACAT TTAGAAATCT TGAAGAAGAT GGGGGTGAGT
    TTGATTCACC TCAAAGATGG GAGAGTCCAG TTGGTTCAAC ATGCAATCCA AGCTGCTAGT
    TCAATTGATG CTGAGGATGG TTTGAGGTTC ATGCAGGAGA TGATCGAATT GTCAAGTCAG
    CAACAAAAAG AACAGCAGCT GCCTCCACGA GCTGTTCAAA TTGTATCCCA CCCATTTTTT
    GGCAGGGTAG TATTGACTGC TGGACCAGCT CAGTTTGGGC TGGATCTGTC TAAACATAAA
    GAGACAAGAG GATTTGTTGC AAGCAGTAAA CCATACAATG GTTGTTCAGA GCTTACTAAC
    CCCGAGGCAG TGATGGGAAA AATCGCTTTG ATACAAAGAG GACAGTGCAT GTTTGCAGAA
    AAGGCACGCA ACATTCAGAA TGCTGGTGCC ATTGGTGGCA TTGTTATTGA TGACAATGAG
    GGGAGCAGCA GTGATACTGC CCCTCTGTTC CAGATGGCAG GTGATGGAAA AGACACAGAC
    GACATCAAGA TCCCCATGCT ATTCTTATTC AGTAAAGAAG GAAGTATCAT ATTGGATGCC
    ATCCGGGAAT ATGAGGAGGT GGAAGTACTC CTTTCTGACA AAGCAAAAGA TAGAGCAGCA
    ATATTAAAAG ATCCTGAAAT GGAAAATGAA GAACAACCAT CCTCTGAAAA TGATTCTCAG
    AATCAGAGTG CTGAACAGAT TACATCAAGT TCTCAGGAGG TTGATTTGGT GGATCAAGAG
    TCTCCTGAGG AAAGTTCTCT AAACTCACAA CCAGAATCAT CATCTCCAAC AGATACAGAC
    AATGCTGCAA GTGTTTCTGC TTCTGAACAG AATTCTAACC CCGTAGAAAA CCATGAGACT
    ACAAGTCTTG ATGGTGAATG TACAGATTTA GATAACCAAC TTCAAGAACA ATCAGAAACC
    GAAGAAGATT CCAATCCTAA TGTTAGCTGG GGTAAAAAGG TCCAGCCTAT AGACTCCATA
    TTAGCAGACT GGAATGAAGA TATAGAAGCA TTTGAAATGA TGGAGAAAGA CGAACTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013970613.1
    CDS224..3043
    Translation

    Target ORF information:

    RefSeq Version XM_014115138.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ER degradation enhancer, mannosidase alpha-like 3 (EDEM3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014115138.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGCGAAG CCCGCGGCGG CCGGGGCTGT GGGTCCTCGG TCCCCCAGCG AGCGCCATGG 
    AGCCTGGTGG CGGCGGCGGC CGCGCTCTGC CTGGTGGCGG CCACGTCCGT GTGGACGGCG
    GGGGCCGCGC CCATGAGTAG GGAGGAGAGA CAGAAGCTTG GGAATCAAGT ACTGGAAATG
    TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCTGATGA ACTCATGCCT
    TTAACCTGTA GAGGTCGTGT TAGAGGCCAG GAGCCAAGTC GGGGTGATGT TGATGATGCC
    TTGGGAAAAT TTTCCCTGAC ACTGATTGAT TCTTTGGATA CTCTTGTGGT ATTAAATAAA
    ACTAAAGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGAG ATGTTAATTT AGATAATGAT
    GTAGTTGTAT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC
    TCGCTGGCGA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAACTT
    CTCCAAATGG CGAAGCAATT GGGTTACAAA CTTTTACCAG CTTTCAATAC TACCAGTGGC
    CTTCCTTATC CAAGAATTAA TTTAAAATTT GGTATCAGAA AACCGGAAGC TCGAACAGGA
    ACTGAAACAG ATACTTGTAC AGCTTGTGCA GGTACCTTGA TCCTTGAGTT CGCTGCTTTA
    AGTCGATTCA CAGGAGCAAC AATATTTGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC
    TGGGAAAAAA GACAGCGAAG TAGTAATTTA GTGGGTGTGA CTATAAATAT TCATACTGGA
    GACTGGGTAA GAAAAGATAG TGGAGTTGGA GCAGGGATTG ATTCATATTA TGAATATCTG
    TTGAAAGCTT ATGTCTTGCT TGGAGATGAC AGTTTCCTGG AAAGATTTAA TACACACTAT
    GATGCCATAA TGAGGTACAT TAGCCAGCCA CCTCTTCTGC TTGATGTGCA TATCCACAAG
    CCAATGCTGA ATGCTCGGAC TTGGATGGAT GCATTGCTCG CCTTTTTTCC AGGTTTGCAG
    GTCTTAAAGG GGGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA TCAGGTGATT
    AAAAAACATA ACTTCCTACC AGAGGCATTT ACTACAGATT TCAGGGTACA TTGGGCTCAA
    CATCCTTTAA GACCAGAATT TGCAGAAAGT ACCTACTTCT TATATAAAGC TACAGGAGAT
    CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TAAATAAATA TGCTAGAGTG
    CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGTACTGGAA GTCATGAGGA CAGAATGGAT
    TCTTTCTTCT TGGCTGAGAT GTTTAAATAT CTTTACCTAT TATTTGCGGA TAAAGAAGAC
    ATTATTTTTG ACATAGAAGA TTACATCTTT ACAACAGAAG CTCATCTGTT ACCTCTTTGG
    CTCTCTACTA CAAATCAAAG CATCTCTAAG AAGAATACAA CTTCAGAATA TACAGAACTG
    GATGACAGTA ATTTTGATTG GACTTGTCCA AACACTCAGA TTCTCTTCCC TAATGACCCA
    TTATATGCTC AGAGCATTCG TGAACCCTTG AAAAATGTGG TGGATAAGAG CTGTCCTAGA
    GGCATCATCA GAGTCAGAGA GGAGAGTTTC AGGAGTGGAG CTAAACCCCC TCTGAGAGCC
    AGAGATTTCA TGGCCACTAA CCCTGAACAT TTAGAAATCT TGAAGAAGAT GGGGGTGAGT
    TTGATTCACC TCAAAGATGG GAGAGTCCAG TTGGTTCAAC ATGCAATCCA AGCTGCTAGT
    TCAATTGATG CTGAGGATGG TTTGAGGTTC ATGCAGGAGA TGATCGAATT GTCAAGTCAG
    CAACAAAAAG AACAGCAGCT GCCTCCACGA GCTGTTCAAA TTGTATCCCA CCCATTTTTT
    GGCAGGGTAG TATTGACTGC TGGACCAGCT CAGTTTGGGC TGGATCTGTC TAAACATAAA
    GAGACAAGAG GATTTGTTGC AAGCAGTAAA CCATACAATG GTTGTTCAGA GCTTACTAAC
    CCCGAGGCAG TGATGGGAAA AATCGCTTTG ATACAAAGAG GACAGTGCAT GTTTGCAGAA
    AAGGCACGCA ACATTCAGAA TGCTGGTGCC ATTGGTGGCA TTGTTATTGA TGACAATGAG
    GGGAGCAGCA GTGATACTGC CCCTCTGTTC CAGATGGCAG GTGATGGAAA AGACACAGAC
    GACATCAAGA TCCCCATGCT ATTCTTATTC AGTAAAGAAG GAAGTATCAT ATTGGATGCC
    ATCCGGGAAT ATGAGGAGGT GGAAGTACTC CTTTCTGACA AAGCAAAAGA TAGAGCAGCA
    ATATTAAAAG ATCCTGAAAT GGAAAATGAA GAACAACCAT CCTCTGAAAA TGATTCTCAG
    AATCAGAGTG CTGAACAGAT TACATCAAGT TCTCAGGAGG TTGATTTGGT GGATCAAGAG
    TCTCCTGAGG AAAGTTCTCT AAACTCACAA CCAGAATCAT CATCTCCAAC AGATACAGAC
    AATGCTGCAA GTGTTTCTGC TTCTGAACAG AATTCTAACC CCGTAGAAAA CCATGAGACT
    ACAAGTCTTG ATGGTGAATG TACAGATTTA GATAACCAAC TTCAAGAACA ATCAGAAACC
    GAAGAAGATT CCAATCCTAA TGTTAGCTGG GGTAAAAAGG TCCAGCCTAT AGACTCCATA
    TTAGCAGACT GGAATGAAGA TATAGAAGCA TTTGAAATGA TGGAGAAAGA CGAACTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe37428
    Clone ID Related Accession (Same CDS sequence) XM_014115139.1
    Accession Version XM_014115139.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2817bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGCGAAG CCCGCGGCGG CCGGGGCTGT GGGTCCTCGG TCCCCCAGCG AGCGCCATGG 
    AGCCTGGTGG CGGCGGCGGC CGCGCTCTGC CTGGTGGCGG CCACGTCCGT GTGGACGGCG
    GGGGCCGCGC CCATGAGTAG GGAGGAGAGA CAGAAGCTTG GGAATCAAGT ACTGGAAATG
    TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCTGATGA ACTCATGCCT
    TTAACCTGTA GAGGTCGTGT TAGAGGCCAG GAGCCAAGTC GGGGTGATGT TGATGATGCC
    TTGGGAAAAT TTTCCCTGAC ACTGATTGAT TCTTTGGATA CTCTTGTGGT ATTAAATAAA
    ACTAAAGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGAG ATGTTAATTT AGATAATGAT
    GTAGTTGTAT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC
    TCGCTGGCGA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAACTT
    CTCCAAATGG CGAAGCAATT GGGTTACAAA CTTTTACCAG CTTTCAATAC TACCAGTGGC
    CTTCCTTATC CAAGAATTAA TTTAAAATTT GGTATCAGAA AACCGGAAGC TCGAACAGGA
    ACTGAAACAG ATACTTGTAC AGCTTGTGCA GGTACCTTGA TCCTTGAGTT CGCTGCTTTA
    AGTCGATTCA CAGGAGCAAC AATATTTGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC
    TGGGAAAAAA GACAGCGAAG TAGTAATTTA GTGGGTGTGA CTATAAATAT TCATACTGGA
    GACTGGGTAA GAAAAGATAG TGGAGTTGGA GCAGGGATTG ATTCATATTA TGAATATCTG
    TTGAAAGCTT ATGTCTTGCT TGGAGATGAC AGTTTCCTGG AAAGATTTAA TACACACTAT
    GATGCCATAA TGAGGTACAT TAGCCAGCCA CCTCTTCTGC TTGATGTGCA TATCCACAAG
    CCAATGCTGA ATGCTCGGAC TTGGATGGAT GCATTGCTCG CCTTTTTTCC AGGTTTGCAG
    GTCTTAAAGG GGGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA TCAGGTGATT
    AAAAAACATA ACTTCCTACC AGAGGCATTT ACTACAGATT TCAGGGTACA TTGGGCTCAA
    CATCCTTTAA GACCAGAATT TGCAGAAAGT ACCTACTTCT TATATAAAGC TACAGGAGAT
    CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TAAATAAATA TGCTAGAGTG
    CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGTACTGGAA GTCATGAGGA CAGAATGGAT
    TCTTTCTTCT TGGCTGAGAT GTTTAAATAT CTTTACCTAT TATTTGCGGA TAAAGAAGAC
    ATTATTTTTG ACATAGAAGA TTACATCTTT ACAACAGAAG CTCATCTGTT ACCTCTTTGG
    CTCTCTACTA CAAATCAAAG CATCTCTAAG AAGAATACAA CTTCAGAATA TACAGAACTG
    GATGACAGTA ATTTTGATTG GACTTGTCCA AACACTCAGA TTCTCTTCCC TAATGACCCA
    TTATATGCTC AGAGCATTCG TGAACCCTTG AAAAATGTGG TGGATAAGAG CTGTCCTAGA
    GGCATCATCA GAGTCAGAGA GGAGAGTTTC AGGAGTGGAG CTAAACCCCC TCTGAGAGCC
    AGAGATTTCA TGGCCACTAA CCCTGAACAT TTAGAAATCT TGAAGAAGAT GGGGGTGAGT
    TTGATTCACC TCAAAGATGG GAGAGTCCAG TTGGTTCAAC ATGCAATCCA AGCTGCTAGT
    TCAATTGATG CTGAGGATGG TTTGAGGTTC ATGCAGGAGA TGATCGAATT GTCAAGTCAG
    CAACAAAAAG AACAGCAGCT GCCTCCACGA GCTGTTCAAA TTGTATCCCA CCCATTTTTT
    GGCAGGGTAG TATTGACTGC TGGACCAGCT CAGTTTGGGC TGGATCTGTC TAAACATAAA
    GAGACAAGAG GATTTGTTGC AAGCAGTAAA CCATACAATG GTTGTTCAGA GCTTACTAAC
    CCCGAGGCAG TGATGGGAAA AATCGCTTTG ATACAAAGAG GACAGTGCAT GTTTGCAGAA
    AAGGCACGCA ACATTCAGAA TGCTGGTGCC ATTGGTGGCA TTGTTATTGA TGACAATGAG
    GGGAGCAGCA GTGATACTGC CCCTCTGTTC CAGATGGCAG GTGATGGAAA AGACACAGAC
    GACATCAAGA TCCCCATGCT ATTCTTATTC AGTAAAGAAG GAAGTATCAT ATTGGATGCC
    ATCCGGGAAT ATGAGGAGGT GGAAGTACTC CTTTCTGACA AAGCAAAAGA TAGAGCAATA
    TTAAAAGATC CTGAAATGGA AAATGAAGAA CAACCATCCT CTGAAAATGA TTCTCAGAAT
    CAGAGTGCTG AACAGATTAC ATCAAGTTCT CAGGAGGTTG ATTTGGTGGA TCAAGAGTCT
    CCTGAGGAAA GTTCTCTAAA CTCACAACCA GAATCATCAT CTCCAACAGA TACAGACAAT
    GCTGCAAGTG TTTCTGCTTC TGAACAGAAT TCTAACCCCG TAGAAAACCA TGAGACTACA
    AGTCTTGATG GTGAATGTAC AGATTTAGAT AACCAACTTC AAGAACAATC AGAAACCGAA
    GAAGATTCCA ATCCTAATGT TAGCTGGGGT AAAAAGGTCC AGCCTATAGA CTCCATATTA
    GCAGACTGGA ATGAAGATAT AGAAGCATTT GAAATGATGG AGAAAGACGA ACTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013970614.1
    CDS224..3040
    Translation

    Target ORF information:

    RefSeq Version XM_014115139.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ER degradation enhancer, mannosidase alpha-like 3 (EDEM3), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014115139.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGCGAAG CCCGCGGCGG CCGGGGCTGT GGGTCCTCGG TCCCCCAGCG AGCGCCATGG 
    AGCCTGGTGG CGGCGGCGGC CGCGCTCTGC CTGGTGGCGG CCACGTCCGT GTGGACGGCG
    GGGGCCGCGC CCATGAGTAG GGAGGAGAGA CAGAAGCTTG GGAATCAAGT ACTGGAAATG
    TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCTGATGA ACTCATGCCT
    TTAACCTGTA GAGGTCGTGT TAGAGGCCAG GAGCCAAGTC GGGGTGATGT TGATGATGCC
    TTGGGAAAAT TTTCCCTGAC ACTGATTGAT TCTTTGGATA CTCTTGTGGT ATTAAATAAA
    ACTAAAGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGAG ATGTTAATTT AGATAATGAT
    GTAGTTGTAT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC
    TCGCTGGCGA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAACTT
    CTCCAAATGG CGAAGCAATT GGGTTACAAA CTTTTACCAG CTTTCAATAC TACCAGTGGC
    CTTCCTTATC CAAGAATTAA TTTAAAATTT GGTATCAGAA AACCGGAAGC TCGAACAGGA
    ACTGAAACAG ATACTTGTAC AGCTTGTGCA GGTACCTTGA TCCTTGAGTT CGCTGCTTTA
    AGTCGATTCA CAGGAGCAAC AATATTTGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC
    TGGGAAAAAA GACAGCGAAG TAGTAATTTA GTGGGTGTGA CTATAAATAT TCATACTGGA
    GACTGGGTAA GAAAAGATAG TGGAGTTGGA GCAGGGATTG ATTCATATTA TGAATATCTG
    TTGAAAGCTT ATGTCTTGCT TGGAGATGAC AGTTTCCTGG AAAGATTTAA TACACACTAT
    GATGCCATAA TGAGGTACAT TAGCCAGCCA CCTCTTCTGC TTGATGTGCA TATCCACAAG
    CCAATGCTGA ATGCTCGGAC TTGGATGGAT GCATTGCTCG CCTTTTTTCC AGGTTTGCAG
    GTCTTAAAGG GGGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA TCAGGTGATT
    AAAAAACATA ACTTCCTACC AGAGGCATTT ACTACAGATT TCAGGGTACA TTGGGCTCAA
    CATCCTTTAA GACCAGAATT TGCAGAAAGT ACCTACTTCT TATATAAAGC TACAGGAGAT
    CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TAAATAAATA TGCTAGAGTG
    CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGTACTGGAA GTCATGAGGA CAGAATGGAT
    TCTTTCTTCT TGGCTGAGAT GTTTAAATAT CTTTACCTAT TATTTGCGGA TAAAGAAGAC
    ATTATTTTTG ACATAGAAGA TTACATCTTT ACAACAGAAG CTCATCTGTT ACCTCTTTGG
    CTCTCTACTA CAAATCAAAG CATCTCTAAG AAGAATACAA CTTCAGAATA TACAGAACTG
    GATGACAGTA ATTTTGATTG GACTTGTCCA AACACTCAGA TTCTCTTCCC TAATGACCCA
    TTATATGCTC AGAGCATTCG TGAACCCTTG AAAAATGTGG TGGATAAGAG CTGTCCTAGA
    GGCATCATCA GAGTCAGAGA GGAGAGTTTC AGGAGTGGAG CTAAACCCCC TCTGAGAGCC
    AGAGATTTCA TGGCCACTAA CCCTGAACAT TTAGAAATCT TGAAGAAGAT GGGGGTGAGT
    TTGATTCACC TCAAAGATGG GAGAGTCCAG TTGGTTCAAC ATGCAATCCA AGCTGCTAGT
    TCAATTGATG CTGAGGATGG TTTGAGGTTC ATGCAGGAGA TGATCGAATT GTCAAGTCAG
    CAACAAAAAG AACAGCAGCT GCCTCCACGA GCTGTTCAAA TTGTATCCCA CCCATTTTTT
    GGCAGGGTAG TATTGACTGC TGGACCAGCT CAGTTTGGGC TGGATCTGTC TAAACATAAA
    GAGACAAGAG GATTTGTTGC AAGCAGTAAA CCATACAATG GTTGTTCAGA GCTTACTAAC
    CCCGAGGCAG TGATGGGAAA AATCGCTTTG ATACAAAGAG GACAGTGCAT GTTTGCAGAA
    AAGGCACGCA ACATTCAGAA TGCTGGTGCC ATTGGTGGCA TTGTTATTGA TGACAATGAG
    GGGAGCAGCA GTGATACTGC CCCTCTGTTC CAGATGGCAG GTGATGGAAA AGACACAGAC
    GACATCAAGA TCCCCATGCT ATTCTTATTC AGTAAAGAAG GAAGTATCAT ATTGGATGCC
    ATCCGGGAAT ATGAGGAGGT GGAAGTACTC CTTTCTGACA AAGCAAAAGA TAGAGCAATA
    TTAAAAGATC CTGAAATGGA AAATGAAGAA CAACCATCCT CTGAAAATGA TTCTCAGAAT
    CAGAGTGCTG AACAGATTAC ATCAAGTTCT CAGGAGGTTG ATTTGGTGGA TCAAGAGTCT
    CCTGAGGAAA GTTCTCTAAA CTCACAACCA GAATCATCAT CTCCAACAGA TACAGACAAT
    GCTGCAAGTG TTTCTGCTTC TGAACAGAAT TCTAACCCCG TAGAAAACCA TGAGACTACA
    AGTCTTGATG GTGAATGTAC AGATTTAGAT AACCAACTTC AAGAACAATC AGAAACCGAA
    GAAGATTCCA ATCCTAATGT TAGCTGGGGT AAAAAGGTCC AGCCTATAGA CTCCATATTA
    GCAGACTGGA ATGAAGATAT AGAAGCATTT GAAATGATGG AGAAAGACGA ACTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe37429
    Clone ID Related Accession (Same CDS sequence) XM_014115140.1
    Accession Version XM_014115140.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2817bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGCGAAG CCCGCGGCGG CCGGGGCTGT GGGTCCTCGG TCCCCCAGCG AGCGCCATGG 
    AGCCTGGTGG CGGCGGCGGC CGCGCTCTGC CTGGTGGCGG CCACGTCCGT GTGGACGGCG
    GGGGCCGCGC CCATGAGTAG GGAGGAGAGA CAGAAGCTTG GGAATCAAGT ACTGGAAATG
    TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCTGATGA ACTCATGCCT
    TTAACCTGTA GAGGTCGTGT TAGAGGCCAG GAGCCAAGTC GGGGTGATGT TGATGATGCC
    TTGGGAAAAT TTTCCCTGAC ACTGATTGAT TCTTTGGATA CTCTTGTGGT ATTAAATAAA
    ACTAAAGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGAG ATGTTAATTT AGATAATGAT
    GTAGTTGTAT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC
    TCGCTGGCGA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAACTT
    CTCCAAATGG CGAAGCAATT GGGTTACAAA CTTTTACCAG CTTTCAATAC TACCAGTGGC
    CTTCCTTATC CAAGAATTAA TTTAAAATTT GGTATCAGAA AACCGGAAGC TCGAACAGGA
    ACTGAAACAG ATACTTGTAC AGCTTGTGCA GGTACCTTGA TCCTTGAGTT CGCTGCTTTA
    AGTCGATTCA CAGGAGCAAC AATATTTGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC
    TGGGAAAAAA GACAGCGAAG TAGTAATTTA GTGGGTGTGA CTATAAATAT TCATACTGGA
    GACTGGGTAA GAAAAGATAG TGGAGTTGGA GCAGGGATTG ATTCATATTA TGAATATCTG
    TTGAAAGCTT ATGTCTTGCT TGGAGATGAC AGTTTCCTGG AAAGATTTAA TACACACTAT
    GATGCCATAA TGAGGTACAT TAGCCAGCCA CCTCTTCTGC TTGATGTGCA TATCCACAAG
    CCAATGCTGA ATGCTCGGAC TTGGATGGAT GCATTGCTCG CCTTTTTTCC AGGTTTGCAG
    GTCTTAAAGG GGGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA TCAGGTGATT
    AAAAAACATA ACTTCCTACC AGAGGCATTT ACTACAGATT TCAGGGTACA TTGGGCTCAA
    CATCCTTTAA GACCAGAATT TGCAGAAAGT ACCTACTTCT TATATAAAGC TACAGGAGAT
    CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TAAATAAATA TGCTAGAGTG
    CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGTACTGGAA GTCATGAGGA CAGAATGGAT
    TCTTTCTTCT TGGCTGAGAT GTTTAAATAT CTTTACCTAT TATTTGCGGA TAAAGAAGAC
    ATTATTTTTG ACATAGAAGA TTACATCTTT ACAACAGAAG CTCATCTGTT ACCTCTTTGG
    CTCTCTACTA CAAATCAAAG CATCTCTAAG AAGAATACAA CTTCAGAATA TACAGAACTG
    GATGACAGTA ATTTTGATTG GACTTGTCCA AACACTCAGA TTCTCTTCCC TAATGACCCA
    TTATATGCTC AGAGCATTCG TGAACCCTTG AAAAATGTGG TGGATAAGAG CTGTCCTAGA
    GGCATCATCA GAGTAGAGGA GAGTTTCAGG AGTGGAGCTA AACCCCCTCT GAGAGCCAGA
    GATTTCATGG CCACTAACCC TGAACATTTA GAAATCTTGA AGAAGATGGG GGTGAGTTTG
    ATTCACCTCA AAGATGGGAG AGTCCAGTTG GTTCAACATG CAATCCAAGC TGCTAGTTCA
    ATTGATGCTG AGGATGGTTT GAGGTTCATG CAGGAGATGA TCGAATTGTC AAGTCAGCAA
    CAAAAAGAAC AGCAGCTGCC TCCACGAGCT GTTCAAATTG TATCCCACCC ATTTTTTGGC
    AGGGTAGTAT TGACTGCTGG ACCAGCTCAG TTTGGGCTGG ATCTGTCTAA ACATAAAGAG
    ACAAGAGGAT TTGTTGCAAG CAGTAAACCA TACAATGGTT GTTCAGAGCT TACTAACCCC
    GAGGCAGTGA TGGGAAAAAT CGCTTTGATA CAAAGAGGAC AGTGCATGTT TGCAGAAAAG
    GCACGCAACA TTCAGAATGC TGGTGCCATT GGTGGCATTG TTATTGATGA CAATGAGGGG
    AGCAGCAGTG ATACTGCCCC TCTGTTCCAG ATGGCAGGTG ATGGAAAAGA CACAGACGAC
    ATCAAGATCC CCATGCTATT CTTATTCAGT AAAGAAGGAA GTATCATATT GGATGCCATC
    CGGGAATATG AGGAGGTGGA AGTACTCCTT TCTGACAAAG CAAAAGATAG AGCAGCAATA
    TTAAAAGATC CTGAAATGGA AAATGAAGAA CAACCATCCT CTGAAAATGA TTCTCAGAAT
    CAGAGTGCTG AACAGATTAC ATCAAGTTCT CAGGAGGTTG ATTTGGTGGA TCAAGAGTCT
    CCTGAGGAAA GTTCTCTAAA CTCACAACCA GAATCATCAT CTCCAACAGA TACAGACAAT
    GCTGCAAGTG TTTCTGCTTC TGAACAGAAT TCTAACCCCG TAGAAAACCA TGAGACTACA
    AGTCTTGATG GTGAATGTAC AGATTTAGAT AACCAACTTC AAGAACAATC AGAAACCGAA
    GAAGATTCCA ATCCTAATGT TAGCTGGGGT AAAAAGGTCC AGCCTATAGA CTCCATATTA
    GCAGACTGGA ATGAAGATAT AGAAGCATTT GAAATGATGG AGAAAGACGA ACTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013970615.1
    CDS224..3040
    Translation

    Target ORF information:

    RefSeq Version XM_014115140.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ER degradation enhancer, mannosidase alpha-like 3 (EDEM3), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014115140.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGCGAAG CCCGCGGCGG CCGGGGCTGT GGGTCCTCGG TCCCCCAGCG AGCGCCATGG 
    AGCCTGGTGG CGGCGGCGGC CGCGCTCTGC CTGGTGGCGG CCACGTCCGT GTGGACGGCG
    GGGGCCGCGC CCATGAGTAG GGAGGAGAGA CAGAAGCTTG GGAATCAAGT ACTGGAAATG
    TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCTGATGA ACTCATGCCT
    TTAACCTGTA GAGGTCGTGT TAGAGGCCAG GAGCCAAGTC GGGGTGATGT TGATGATGCC
    TTGGGAAAAT TTTCCCTGAC ACTGATTGAT TCTTTGGATA CTCTTGTGGT ATTAAATAAA
    ACTAAAGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGAG ATGTTAATTT AGATAATGAT
    GTAGTTGTAT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC
    TCGCTGGCGA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAACTT
    CTCCAAATGG CGAAGCAATT GGGTTACAAA CTTTTACCAG CTTTCAATAC TACCAGTGGC
    CTTCCTTATC CAAGAATTAA TTTAAAATTT GGTATCAGAA AACCGGAAGC TCGAACAGGA
    ACTGAAACAG ATACTTGTAC AGCTTGTGCA GGTACCTTGA TCCTTGAGTT CGCTGCTTTA
    AGTCGATTCA CAGGAGCAAC AATATTTGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC
    TGGGAAAAAA GACAGCGAAG TAGTAATTTA GTGGGTGTGA CTATAAATAT TCATACTGGA
    GACTGGGTAA GAAAAGATAG TGGAGTTGGA GCAGGGATTG ATTCATATTA TGAATATCTG
    TTGAAAGCTT ATGTCTTGCT TGGAGATGAC AGTTTCCTGG AAAGATTTAA TACACACTAT
    GATGCCATAA TGAGGTACAT TAGCCAGCCA CCTCTTCTGC TTGATGTGCA TATCCACAAG
    CCAATGCTGA ATGCTCGGAC TTGGATGGAT GCATTGCTCG CCTTTTTTCC AGGTTTGCAG
    GTCTTAAAGG GGGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA TCAGGTGATT
    AAAAAACATA ACTTCCTACC AGAGGCATTT ACTACAGATT TCAGGGTACA TTGGGCTCAA
    CATCCTTTAA GACCAGAATT TGCAGAAAGT ACCTACTTCT TATATAAAGC TACAGGAGAT
    CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TAAATAAATA TGCTAGAGTG
    CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGTACTGGAA GTCATGAGGA CAGAATGGAT
    TCTTTCTTCT TGGCTGAGAT GTTTAAATAT CTTTACCTAT TATTTGCGGA TAAAGAAGAC
    ATTATTTTTG ACATAGAAGA TTACATCTTT ACAACAGAAG CTCATCTGTT ACCTCTTTGG
    CTCTCTACTA CAAATCAAAG CATCTCTAAG AAGAATACAA CTTCAGAATA TACAGAACTG
    GATGACAGTA ATTTTGATTG GACTTGTCCA AACACTCAGA TTCTCTTCCC TAATGACCCA
    TTATATGCTC AGAGCATTCG TGAACCCTTG AAAAATGTGG TGGATAAGAG CTGTCCTAGA
    GGCATCATCA GAGTAGAGGA GAGTTTCAGG AGTGGAGCTA AACCCCCTCT GAGAGCCAGA
    GATTTCATGG CCACTAACCC TGAACATTTA GAAATCTTGA AGAAGATGGG GGTGAGTTTG
    ATTCACCTCA AAGATGGGAG AGTCCAGTTG GTTCAACATG CAATCCAAGC TGCTAGTTCA
    ATTGATGCTG AGGATGGTTT GAGGTTCATG CAGGAGATGA TCGAATTGTC AAGTCAGCAA
    CAAAAAGAAC AGCAGCTGCC TCCACGAGCT GTTCAAATTG TATCCCACCC ATTTTTTGGC
    AGGGTAGTAT TGACTGCTGG ACCAGCTCAG TTTGGGCTGG ATCTGTCTAA ACATAAAGAG
    ACAAGAGGAT TTGTTGCAAG CAGTAAACCA TACAATGGTT GTTCAGAGCT TACTAACCCC
    GAGGCAGTGA TGGGAAAAAT CGCTTTGATA CAAAGAGGAC AGTGCATGTT TGCAGAAAAG
    GCACGCAACA TTCAGAATGC TGGTGCCATT GGTGGCATTG TTATTGATGA CAATGAGGGG
    AGCAGCAGTG ATACTGCCCC TCTGTTCCAG ATGGCAGGTG ATGGAAAAGA CACAGACGAC
    ATCAAGATCC CCATGCTATT CTTATTCAGT AAAGAAGGAA GTATCATATT GGATGCCATC
    CGGGAATATG AGGAGGTGGA AGTACTCCTT TCTGACAAAG CAAAAGATAG AGCAGCAATA
    TTAAAAGATC CTGAAATGGA AAATGAAGAA CAACCATCCT CTGAAAATGA TTCTCAGAAT
    CAGAGTGCTG AACAGATTAC ATCAAGTTCT CAGGAGGTTG ATTTGGTGGA TCAAGAGTCT
    CCTGAGGAAA GTTCTCTAAA CTCACAACCA GAATCATCAT CTCCAACAGA TACAGACAAT
    GCTGCAAGTG TTTCTGCTTC TGAACAGAAT TCTAACCCCG TAGAAAACCA TGAGACTACA
    AGTCTTGATG GTGAATGTAC AGATTTAGAT AACCAACTTC AAGAACAATC AGAAACCGAA
    GAAGATTCCA ATCCTAATGT TAGCTGGGGT AAAAAGGTCC AGCCTATAGA CTCCATATTA
    GCAGACTGGA ATGAAGATAT AGAAGCATTT GAAATGATGG AGAAAGACGA ACTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.