TSC2 cDNA ORF clone, Canis lupus familiaris(dog)

The following TSC2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TSC2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe289103 XM_022420250.1
Latest version!
Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe289104 XM_022420251.1
Latest version!
Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe289105 XM_022420252.1
Latest version!
Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe37159 XM_014114692.2
Latest version!
Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe08328 XM_005621715.3
Latest version!
Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe08329 XM_005621716.3
Latest version!
Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe08331 XM_005621717.3
Latest version!
Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe08333 XM_005621718.3
Latest version!
Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe08334 XM_005621719.3
Latest version!
Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe08335 XM_005621720.3
Latest version!
Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
View Old Accession Versions >>
OCe08334 XM_005621719.2 Canis lupus familiaris tuberous sclerosis 2 (TSC2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe08329 XM_005621716.2 Canis lupus familiaris tuberous sclerosis 2 (TSC2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe08331 XM_005621717.2 Canis lupus familiaris tuberous sclerosis 2 (TSC2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe08328 XM_005621715.2 Canis lupus familiaris tuberous sclerosis 2 (TSC2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe08333 XM_005621718.2 Canis lupus familiaris tuberous sclerosis 2 (TSC2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe08335 XM_005621720.2 Canis lupus familiaris tuberous sclerosis 2 (TSC2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe37159 XM_014114692.1 Canis lupus familiaris tuberous sclerosis 2 (TSC2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe289103
    Clone ID Related Accession (Same CDS sequence) XM_022420250.1
    Accession Version XM_022420250.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4941bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product tuberin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACATCG GCCTGTCCTC CGAATTCCTC CTGGTGCTTG TGAACTTGGT CAAGTTCAAC 
    AGCTGTTACC TGGATGAGTA CATTGCATCA ATGGTCCACA TGATCTGCCT GCTGTGCGTC
    CAGACCGTGT CATCCGTGGA CATAGAGGTC TCCCTGCAGG TGCTGGACGC CGTCGTCTGC
    TACAACTGCC TGCCGGCTGA GAGCCTCCCC CTGTTCATCG TCACCCTGTG CCGCACCATC
    AACGTGAAGG AGCTCTGTGA GCCTTGCTGG AAGCTGATGC GGAACCTGCT GGGCACCCAC
    CTGGGCCACA GTGCCATCTA CAACATGTGT CGCATCATGG AGGACAGAGC CTACATGGAA
    GATGCCCCAC TGCTGAGAGG GGCTGTGTTC TTTGTGGGGA TGGCCCTTTG GGGAGCACAC
    CGACTCTACT CTCTCAAGAA CTCCCCAACG TCTGTGTTGC CGTCGTTTTA TGAGGCTATG
    ACCTGTCCGA ATGAAGTGGT GTCCTATGAA ATCGTCCTCT CTATAACCAG ACTCATCAAG
    AAGTACCGGA GGGAGCTGCA AGCTGTGACC TGGGACATCC TACTGAACAT CATGGAGCGC
    CTCCTGCACC AGCTCCAGAG CCTGGACAGC CCGGAGCTCA GAGCCATCGT GCACGACCTG
    CTGACCACAG TGGAAGAGCT GTGTGATCAG AATGAGTTCC ACGGCTCTCA GGAGAGATAC
    TTTGAGCTGG TTGAGCGATG TGCAGACCAG AGGCCGGAGT CTTCCCTCTT AAACTTAATA
    ACGTACCGGG CTCAGTCAAT CCACCCGGCG AAGGATGGCT GGATTCAGAA CCTGCAGTTA
    CTGATGGAGA GGTTCTTCAG GAACGAATCT CGCAGTGCTG TGCGCATCAA GGTGCTGGAC
    GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTATG AGGAGGAGCT GATCAGCTCG
    GTGGTCATCT CACAGCTCTC CCATGTCCCG GAGGACAAAG ACCCCCAAGT CCGGAAACTG
    GCCACCCAGT TGCTGGTGGA CCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGTCTG
    TTGGACATTG TGGAAAAGGT GATTGCCCGC TCTCTCTCTC CACCCCCTGA GCTGGAGGAA
    AGAGACGTGG CGGTGTACTC GGCCTCCTTA GAGGATGTGA AGACTGCTGT CCTGGGGCTC
    CTGGTCATCC TTCAGACCAA GCTGTACGCC TTGCCTGCCA GCCATGCGAT GCGCGTGTAC
    GAGACACTCG TCAGCCACAT CCAGCTCCAC TACAAGCATA GCTATACCCT GCCCATTGCC
    AGTAGCATCC GGCTGCAGGC TTTTGACTTT CTACTGCTGC TGAGGGCCGA CTCGCTGCAC
    CGCCTCGGCC TGCCCAGCAA GGACGGGGCC GTGAGGTTCA GCCCTTACTG CGTCTGCGAT
    GCCATGGAGC CAGAGAGAGG CTCAGAGAAG AAGGCCAGTG GCCCCCTGTC ACCTCCCACG
    GGGCCTCCTG GTCCTGCGCC CGCAGGCCCC GCCGTGCGGC TTGGCTCCCT GCCCTACTCC
    CTGCTCTTCC GTGTCCTGCT GCAGTGCCTG AAGCTGGAGG TGGACTGGAA GGTACTGAAG
    CTCGTGCTTA GCAAGCTGCC AGAGTCCCTG CGTTATAAGG TGCTCATCTT CACCTCGCCC
    TGCAGCGTGG ACCAGCTGTC CTCCGCCCTC TGCTCCATGC TCTCAGGTCC CAGAACTCTG
    GAGCGGCTCC GAGGCACCCC GGAAGGGTTC TCCAGAACCG ACCTGCACCT GGCTGTGGTC
    CCTGTGCTGA CGGCGCTCAT CTCTTACCAT AACTACCTGG ACAAAACCAG ACAGCGGGAG
    ATGGTGTACT GCCTGGAGCA AGGCCTGATC TACCGCTGCG CCAGCCAGTG TGTGGTGGCC
    CTGGCCATCT GCAGCGTGGA GATGCCTGAC ATCATCATCA AGGCGCTTCC CGTCCTGGTC
    GTGAAGCTCA CACACATCTC GGCCACGGCC AGTATGGCCA TTCCACTCCT CGAGTTCCTG
    TCAACGCTGG CCAGGTTGCC CCACCTGTAC AGGAACTTCG CAGCAGAGCA GTACGCGAGT
    GTGTTTGCCA TCTCCCTGCC CTACACCAAT CCCTCCAAGT TCAATCAGTA CATCGTGTGC
    CTGGCCCATC ATGTCATAGC CATGTGGTTC ATTAGATGCC GCCTGCCCTT CCGGAAGGAT
    TTTGTTCCTT ATATCACTAA GGGCCTGCGC TCCAACGTCC TCCTGTCTTT TGATGACACC
    CCCGAGAAGG ACAGTTTCAG AGCACGAAGC ACCAGTCTCA ACGAGAGGCC CAAGAGTTTG
    AGGATAGCCA GACCCCCCAA ACAAGGCTTG AATAACTCTC CACCCGTGAA AGAATTCAAA
    GAGAGCTCTG CCGCCGAGGC CTTCCGATGC CGCAGCATCA GTGTGTCTGA ACATGTGGTC
    CGCAGCAGGA TCCAGACGTC CCTCACAAGC GCCAGCTTGG GGTCTGCTGA CGAGAACTCG
    ATGGCCCAGG CTGATGACAA CTTGAAAAAT CTCCACCTGG AGCTCACGGA AACATGTCTG
    GACATGATGG CCAGATATGT GTTCTCCAAC TTCACAGCAG TCCCCAAGAG GTCCCCGGTG
    GGAGAGTTCC TCCTGGCTGG TGGCAGGACC AAAACCTGGC TGGTTGGGAA CAAGCTTGTC
    ACTGTGACGA CGAGCGTGGG GACGGGGACC CGGTCGCTGC TGGGCTTGGA CTCAGGAGAT
    CAGCAAGGTG GCCCCGAGTT GAGCTCGGAC CCTGGCATGC ATGTGAGACA GGCAAAGGAG
    GCACCTGCCA AGCTGGAGTC GCAGGCTGGG CAGCAGGTGT GCCGCGGCGC CCGGGACCGG
    GTCCGCTCCA TGTCCGGGGG CCACGGTCTT CGTGTTGGCG CCCTGGACAC TCCAGCCTCC
    CACTTGCCGG GTGGCCCCAC TTCCCCAGGC ACGCAGGCAG CGCTGGCCAG CAAGTCTGAC
    AGGGCCTCGG CTGGCACCCG GTTCCCAGCA CAGGAGAAGA CGAGCCTGGC AGCCTACGTA
    CCGCTGCTGA CCCAGGGCTG GGCGGAGATC TTGGTCCGGA GGCCTACAGG GAACACCAGC
    TGGCTGATGA GCCTGGAGAA CCCGCTCAGC CCCTTCTCGT CGGACATCAA CAACATGCCT
    CTGCAGGAGC TGTCCAACGC GCTCATGGCC GCTGAGCGCT TCAAGGAGCG TCGTGACACG
    GCTCTGTACA AGTCGCTGTC GGTGCCTGCC GCAGGCTCGG CCAAGCCCCC TCCGCCCCCG
    AGATCTAACA CGGTGGCCTC TTTCTCCTCC CTGTACCAGT CCAGTTGCCA AGGAAAGCTG
    CACAGGAGCA TTTCCTGGGC AGACTCTGCG GTGGTTCTGG AGGAGGGAAA TTCAAGCGAG
    ACCAGTCTGC CAGTGGAGCA CACGGAGCTG GAGGACTTCG AGGCGACACT AGGCACAGAC
    CGGCGCTGTG GCCGCGCGGA AGCTTACAGC AGGTCGTCTT CAAGCTCCAG CCAGGAGGAG
    AAGTTCCCTG CAGAGGAGCT GGCTGCCGGA GGGATCCCCA TCGAACGAGC TGTCTCTTCC
    GAGGGTGCCC GGCCTTCTGT GGAGCTCTCC TTCCAGCCCT CCCAGCCCCT CAGCAAGTCC
    AGCTCCTCCC CTGAGCTGCA GACCCTGCAG GACATACTCG GGGACCCCGG GGACAAGTCT
    GATGTTGGCC GGCTCAGCCC TGAGGCCAAG GCCCGGTCAC AGTCCGGGAT TCTGGACGGG
    GTGGGTGCCT CCTGGTCAGC CCCAGGTGAA GAGAACCAGG GCCAGGGCCC CGCACAGCCC
    GAGGGTCCCT TGCCTTCCAG CTGCCCCCGC TCCCCCAGTG GCCTGCGGCC CAGAGGCTAC
    ACCATCTCTG ATTCAGCCCC GTCACGCAGA GGCAAGAGGG TAGAGCGGGA CGCCTTCAGG
    AGCAGAGCTG GGGCTTCCAA CACTGAGAAG GTGCCAGGCA TCAATCCCAG CTTTGTGTTC
    CTGCAGCTCT ACCACTCACC CTTCTTTGGT GACGAGTCCA ACAAGCCCAT CCTTCTACCC
    AATGAGTCCT TCGAGCGGTC AGTGCAGCTT CTCGACCAGA TTCCATCATA CGACACACAC
    AAGATTGCCG TCCTGTACGT GGGAGAAGGC CAGAGCAGCA GTGAGCTCGC CATCCTGTCC
    AATGAGCATG GCTCGTACAG GTACACGGAG TTCCTGACAG GCCTGGGCAA GCTCATTGAG
    CTCAAAGACT GCCAGCCGGA CAAGGTGTAC CTGGGGGGCC TGGACGTGTG TGGCGAAGAT
    GGCCAGTTCA CCTACTGCTG GCATGACGAC ATCATGCAAG CCGTCTTCCA CATTGCTACC
    CTGATGCCCA CCAAGGAAGT GGACAAGCAC CGCTGTGACA AGAAGCGCCA CCTGGGCAAT
    GACTTTGTGT CCATCGTCTA CAACGACTCT GGTGAGGACT TCAAGCTGGG CACCATCCGA
    GGCCAGTTCA ACTTCGTCCA CGTGATCATC ACCCCCCTGG ACTATGAGTG CAACCTGGTG
    TCCCTGCAGT GCAGGAAAGA CATGGAAGGT CTTGTGGATA CTAGCGTGGC CAAGATCGTG
    TCTGACCGCA ACTTGCCTTT TGTGGCACGT CAGATGGCTT TGCATGCAAA TATGGCCTCA
    CAAGTGCATC ATAGCCGCTC CAACCCCACC GACATCTATC CCTCCAAGTG GATTGCCAGG
    CTTCGCCACA TCAAGCGACT CCGCCACCGG ATCCGCGAGG AAGCCCACTA CACCAACCCC
    AGCCTGCCAC TGATGCAGAT GCACCCCCCG GGCCACCCCA AAGCCTCTGC TCAGGCGCCG
    GCAGAGCCTG TGCCCACGTA TGAGACAGGC CAGCGGAAGC GTCTCATCTC CTCTGTGGAT
    GACTTCACGG AGTTTGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022275958.1
    CDS585..5525
    Translation

    Target ORF information:

    RefSeq Version XM_022420250.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022420250.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACATCG GCCTGTCCTC CGAATTCCTC CTGGTGCTTG TGAACTTGGT CAAGTTCAAC 
    AGCTGTTACC TGGATGAGTA CATTGCATCA ATGGTCCACA TGATCTGCCT GCTGTGCGTC
    CAGACCGTGT CATCCGTGGA CATAGAGGTC TCCCTGCAGG TGCTGGACGC CGTCGTCTGC
    TACAACTGCC TGCCGGCTGA GAGCCTCCCC CTGTTCATCG TCACCCTGTG CCGCACCATC
    AACGTGAAGG AGCTCTGTGA GCCTTGCTGG AAGCTGATGC GGAACCTGCT GGGCACCCAC
    CTGGGCCACA GTGCCATCTA CAACATGTGT CGCATCATGG AGGACAGAGC CTACATGGAA
    GATGCCCCAC TGCTGAGAGG GGCTGTGTTC TTTGTGGGGA TGGCCCTTTG GGGAGCACAC
    CGACTCTACT CTCTCAAGAA CTCCCCAACG TCTGTGTTGC CGTCGTTTTA TGAGGCTATG
    ACCTGTCCGA ATGAAGTGGT GTCCTATGAA ATCGTCCTCT CTATAACCAG ACTCATCAAG
    AAGTACCGGA GGGAGCTGCA AGCTGTGACC TGGGACATCC TACTGAACAT CATGGAGCGC
    CTCCTGCACC AGCTCCAGAG CCTGGACAGC CCGGAGCTCA GAGCCATCGT GCACGACCTG
    CTGACCACAG TGGAAGAGCT GTGTGATCAG AATGAGTTCC ACGGCTCTCA GGAGAGATAC
    TTTGAGCTGG TTGAGCGATG TGCAGACCAG AGGCCGGAGT CTTCCCTCTT AAACTTAATA
    ACGTACCGGG CTCAGTCAAT CCACCCGGCG AAGGATGGCT GGATTCAGAA CCTGCAGTTA
    CTGATGGAGA GGTTCTTCAG GAACGAATCT CGCAGTGCTG TGCGCATCAA GGTGCTGGAC
    GTCCTGTCCT TCGTGCTGCT CATCAACAGG CAGTTCTATG AGGAGGAGCT GATCAGCTCG
    GTGGTCATCT CACAGCTCTC CCATGTCCCG GAGGACAAAG ACCCCCAAGT CCGGAAACTG
    GCCACCCAGT TGCTGGTGGA CCTGGCCGAA GGCTGCCACA CCCACCACTT CAACAGTCTG
    TTGGACATTG TGGAAAAGGT GATTGCCCGC TCTCTCTCTC CACCCCCTGA GCTGGAGGAA
    AGAGACGTGG CGGTGTACTC GGCCTCCTTA GAGGATGTGA AGACTGCTGT CCTGGGGCTC
    CTGGTCATCC TTCAGACCAA GCTGTACGCC TTGCCTGCCA GCCATGCGAT GCGCGTGTAC
    GAGACACTCG TCAGCCACAT CCAGCTCCAC TACAAGCATA GCTATACCCT GCCCATTGCC
    AGTAGCATCC GGCTGCAGGC TTTTGACTTT CTACTGCTGC TGAGGGCCGA CTCGCTGCAC
    CGCCTCGGCC TGCCCAGCAA GGACGGGGCC GTGAGGTTCA GCCCTTACTG CGTCTGCGAT
    GCCATGGAGC CAGAGAGAGG CTCAGAGAAG AAGGCCAGTG GCCCCCTGTC ACCTCCCACG
    GGGCCTCCTG GTCCTGCGCC CGCAGGCCCC GCCGTGCGGC TTGGCTCCCT GCCCTACTCC
    CTGCTCTTCC GTGTCCTGCT GCAGTGCCTG AAGCTGGAGG TGGACTGGAA GGTACTGAAG
    CTCGTGCTTA GCAAGCTGCC AGAGTCCCTG CGTTATAAGG TGCTCATCTT CACCTCGCCC
    TGCAGCGTGG ACCAGCTGTC CTCCGCCCTC TGCTCCATGC TCTCAGGTCC CAGAACTCTG
    GAGCGGCTCC GAGGCACCCC GGAAGGGTTC TCCAGAACCG ACCTGCACCT GGCTGTGGTC
    CCTGTGCTGA CGGCGCTCAT CTCTTACCAT AACTACCTGG ACAAAACCAG ACAGCGGGAG
    ATGGTGTACT GCCTGGAGCA AGGCCTGATC TACCGCTGCG CCAGCCAGTG TGTGGTGGCC
    CTGGCCATCT GCAGCGTGGA GATGCCTGAC ATCATCATCA AGGCGCTTCC CGTCCTGGTC
    GTGAAGCTCA CACACATCTC GGCCACGGCC AGTATGGCCA TTCCACTCCT CGAGTTCCTG
    TCAACGCTGG CCAGGTTGCC CCACCTGTAC AGGAACTTCG CAGCAGAGCA GTACGCGAGT
    GTGTTTGCCA TCTCCCTGCC CTACACCAAT CCCTCCAAGT TCAATCAGTA CATCGTGTGC
    CTGGCCCATC ATGTCATAGC CATGTGGTTC ATTAGATGCC GCCTGCCCTT CCGGAAGGAT
    TTTGTTCCTT ATATCACTAA GGGCCTGCGC TCCAACGTCC TCCTGTCTTT TGATGACACC
    CCCGAGAAGG ACAGTTTCAG AGCACGAAGC ACCAGTCTCA ACGAGAGGCC CAAGAGTTTG
    AGGATAGCCA GACCCCCCAA ACAAGGCTTG AATAACTCTC CACCCGTGAA AGAATTCAAA
    GAGAGCTCTG CCGCCGAGGC CTTCCGATGC CGCAGCATCA GTGTGTCTGA ACATGTGGTC
    CGCAGCAGGA TCCAGACGTC CCTCACAAGC GCCAGCTTGG GGTCTGCTGA CGAGAACTCG
    ATGGCCCAGG CTGATGACAA CTTGAAAAAT CTCCACCTGG AGCTCACGGA AACATGTCTG
    GACATGATGG CCAGATATGT GTTCTCCAAC TTCACAGCAG TCCCCAAGAG GTCCCCGGTG
    GGAGAGTTCC TCCTGGCTGG TGGCAGGACC AAAACCTGGC TGGTTGGGAA CAAGCTTGTC
    ACTGTGACGA CGAGCGTGGG GACGGGGACC CGGTCGCTGC TGGGCTTGGA CTCAGGAGAT
    CAGCAAGGTG GCCCCGAGTT GAGCTCGGAC CCTGGCATGC ATGTGAGACA GGCAAAGGAG
    GCACCTGCCA AGCTGGAGTC GCAGGCTGGG CAGCAGGTGT GCCGCGGCGC CCGGGACCGG
    GTCCGCTCCA TGTCCGGGGG CCACGGTCTT CGTGTTGGCG CCCTGGACAC TCCAGCCTCC
    CACTTGCCGG GTGGCCCCAC TTCCCCAGGC ACGCAGGCAG CGCTGGCCAG CAAGTCTGAC
    AGGGCCTCGG CTGGCACCCG GTTCCCAGCA CAGGAGAAGA CGAGCCTGGC AGCCTACGTA
    CCGCTGCTGA CCCAGGGCTG GGCGGAGATC TTGGTCCGGA GGCCTACAGG GAACACCAGC
    TGGCTGATGA GCCTGGAGAA CCCGCTCAGC CCCTTCTCGT CGGACATCAA CAACATGCCT
    CTGCAGGAGC TGTCCAACGC GCTCATGGCC GCTGAGCGCT TCAAGGAGCG TCGTGACACG
    GCTCTGTACA AGTCGCTGTC GGTGCCTGCC GCAGGCTCGG CCAAGCCCCC TCCGCCCCCG
    AGATCTAACA CGGTGGCCTC TTTCTCCTCC CTGTACCAGT CCAGTTGCCA AGGAAAGCTG
    CACAGGAGCA TTTCCTGGGC AGACTCTGCG GTGGTTCTGG AGGAGGGAAA TTCAAGCGAG
    ACCAGTCTGC CAGTGGAGCA CACGGAGCTG GAGGACTTCG AGGCGACACT AGGCACAGAC
    CGGCGCTGTG GCCGCGCGGA AGCTTACAGC AGGTCGTCTT CAAGCTCCAG CCAGGAGGAG
    AAGTTCCCTG CAGAGGAGCT GGCTGCCGGA GGGATCCCCA TCGAACGAGC TGTCTCTTCC
    GAGGGTGCCC GGCCTTCTGT GGAGCTCTCC TTCCAGCCCT CCCAGCCCCT CAGCAAGTCC
    AGCTCCTCCC CTGAGCTGCA GACCCTGCAG GACATACTCG GGGACCCCGG GGACAAGTCT
    GATGTTGGCC GGCTCAGCCC TGAGGCCAAG GCCCGGTCAC AGTCCGGGAT TCTGGACGGG
    GTGGGTGCCT CCTGGTCAGC CCCAGGTGAA GAGAACCAGG GCCAGGGCCC CGCACAGCCC
    GAGGGTCCCT TGCCTTCCAG CTGCCCCCGC TCCCCCAGTG GCCTGCGGCC CAGAGGCTAC
    ACCATCTCTG ATTCAGCCCC GTCACGCAGA GGCAAGAGGG TAGAGCGGGA CGCCTTCAGG
    AGCAGAGCTG GGGCTTCCAA CACTGAGAAG GTGCCAGGCA TCAATCCCAG CTTTGTGTTC
    CTGCAGCTCT ACCACTCACC CTTCTTTGGT GACGAGTCCA ACAAGCCCAT CCTTCTACCC
    AATGAGTCCT TCGAGCGGTC AGTGCAGCTT CTCGACCAGA TTCCATCATA CGACACACAC
    AAGATTGCCG TCCTGTACGT GGGAGAAGGC CAGAGCAGCA GTGAGCTCGC CATCCTGTCC
    AATGAGCATG GCTCGTACAG GTACACGGAG TTCCTGACAG GCCTGGGCAA GCTCATTGAG
    CTCAAAGACT GCCAGCCGGA CAAGGTGTAC CTGGGGGGCC TGGACGTGTG TGGCGAAGAT
    GGCCAGTTCA CCTACTGCTG GCATGACGAC ATCATGCAAG CCGTCTTCCA CATTGCTACC
    CTGATGCCCA CCAAGGAAGT GGACAAGCAC CGCTGTGACA AGAAGCGCCA CCTGGGCAAT
    GACTTTGTGT CCATCGTCTA CAACGACTCT GGTGAGGACT TCAAGCTGGG CACCATCCGA
    GGCCAGTTCA ACTTCGTCCA CGTGATCATC ACCCCCCTGG ACTATGAGTG CAACCTGGTG
    TCCCTGCAGT GCAGGAAAGA CATGGAAGGT CTTGTGGATA CTAGCGTGGC CAAGATCGTG
    TCTGACCGCA ACTTGCCTTT TGTGGCACGT CAGATGGCTT TGCATGCAAA TATGGCCTCA
    CAAGTGCATC ATAGCCGCTC CAACCCCACC GACATCTATC CCTCCAAGTG GATTGCCAGG
    CTTCGCCACA TCAAGCGACT CCGCCACCGG ATCCGCGAGG AAGCCCACTA CACCAACCCC
    AGCCTGCCAC TGATGCAGAT GCACCCCCCG GGCCACCCCA AAGCCTCTGC TCAGGCGCCG
    GCAGAGCCTG TGCCCACGTA TGAGACAGGC CAGCGGAAGC GTCTCATCTC CTCTGTGGAT
    GACTTCACGG AGTTTGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe289104
    Clone ID Related Accession (Same CDS sequence) XM_022420251.1
    Accession Version XM_022420251.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4842bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product tuberin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG 
    GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC
    GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG
    CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG
    GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG
    ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG
    CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC
    TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC
    CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC
    AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC
    CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG
    TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC
    TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT
    GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA
    GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT
    CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG
    AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC
    AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT
    AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG
    CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC
    AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT
    GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG
    CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG
    GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG
    GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG
    CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC
    GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG
    GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC
    GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC
    ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC
    GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG
    TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC
    CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC
    CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC
    AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT
    CCACCCGTGA AAGAATTCAA AGAGAGCTCT GCCGCCGAGG CCTTCCGATG CCGCAGCATC
    AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATCCAGACGT CCCTCACAAG CGCCAGCTTG
    GGGTCTGCTG ACGAGAACTC GATGGCCCAG GCTGATGACA ACTTGAAAAA TCTCCACCTG
    GAGCTCACGG AAACATGTCT GGACATGATG GCCAGATATG TGTTCTCCAA CTTCACAGCA
    GTCCCCAAGA GGTCCCCGGT GGGAGAGTTC CTCCTGGCTG GTGGCAGGAC CAAAACCTGG
    CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACGAGCGTGG GGACGGGGAC CCGGTCGCTG
    CTGGGCTTGG ACTCAGGAGA TCAGCAAGGT GGCCCCGAGT TGAGCTCGGA CCCTGGCATG
    CATGTGAGAC AGGCAAAGGA GGCACCTGCC AAGCTGGAGT CGCAGGCTGG GCAGCAGGTG
    TGCCGCGGCG CCCGGGACCG GGTCCGCTCC ATGTCCGGGG GCCACGGTCT TCGTGTTGGC
    GCCCTGGACA CTCCAGCCTC CCACTTGCCG GGTGGCCCCA CTTCCCCAGG CACGCAGGCA
    GCGCTGGCCA GCAAGTCTGA CAGGGCCTCG GCTGGCACCC GGTTCCCAGC ACAGGAGAAG
    ACGAGCCTGG CAGCCTACGT ACCGCTGCTG ACCCAGGGCT GGGCGGAGAT CTTGGTCCGG
    AGGCCTACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCCTTCTCG
    TCGGACATCA ACAACATGCC TCTGCAGGAG CTGTCCAACG CGCTCATGGC CGCTGAGCGC
    TTCAAGGAGC GTCGTGACAC GGCTCTGTAC AAGTCGCTGT CGGTGCCTGC CGCAGGCTCG
    GCCAAGCCCC CTCCGCCCCC GAGATCTAAC ACGGTGGCCT CTTTCTCCTC CCTGTACCAG
    TCCAGTTGCC AAGGAAAGCT GCACAGGAGC ATTTCCTGGG CAGACTCTGC GGTGGTTCTG
    GAGGAGGGAA ATTCAAGCGA GACCAGTCTG CCAGTGGAGC ACACGGAGCT GGAGGACTTC
    GAGGCGACAC TAGGCACAGA CCGGCGCTGT GGCCGCGCGG AAGCTTACAG CAGGTCGTCT
    TCAAGCTCCA GCCAGGAGGA GAAGTTCCCT GCAGAGGAGC TGGCTGCCGG AGGGATCCCC
    ATCGAACGAG CTGTCTCTTC CGAGGGTGCC CGGCCTTCTG TGGAGCTCTC CTTCCAGCCC
    TCCCAGCCCC TCAGCAAGTC CAGCTCCTCC CCTGAGCTGC AGACCCTGCA GGACATACTC
    GGGGACCCCG GGGACAAGTC TGATGTTGGC CGGCTCAGCC CTGAGGCCAA GGCCCGGTCA
    CAGTCCGGGA TTCTGGACGG GGTGGGTGCC TCCTGGTCAG CCCCAGGTGA AGAGAACCAG
    GGCCAGGGCC CCGCACAGCC CGAGGGTCCC TTGCCTTCCA GCTGCCCCCG CTCCCCCAGT
    GGCCTGCGGC CCAGAGGCTA CACCATCTCT GATTCAGCCC CGTCACGCAG AGGCAAGAGG
    GTAGAGCGGG ACGCCTTCAG GAGCAGAGCT GGGGCTTCCA ACACTGAGAA GGTGCCAGGC
    ATCAATCCCA GCTTTGTGTT CCTGCAGCTC TACCACTCAC CCTTCTTTGG TGACGAGTCC
    AACAAGCCCA TCCTTCTACC CAATGAGTCC TTCGAGCGGT CAGTGCAGCT TCTCGACCAG
    ATTCCATCAT ACGACACACA CAAGATTGCC GTCCTGTACG TGGGAGAAGG CCAGAGCAGC
    AGTGAGCTCG CCATCCTGTC CAATGAGCAT GGCTCGTACA GGTACACGGA GTTCCTGACA
    GGCCTGGGCA AGCTCATTGA GCTCAAAGAC TGCCAGCCGG ACAAGGTGTA CCTGGGGGGC
    CTGGACGTGT GTGGCGAAGA TGGCCAGTTC ACCTACTGCT GGCATGACGA CATCATGCAA
    GCCGTCTTCC ACATTGCTAC CCTGATGCCC ACCAAGGAAG TGGACAAGCA CCGCTGTGAC
    AAGAAGCGCC ACCTGGGCAA TGACTTTGTG TCCATCGTCT ACAACGACTC TGGTGAGGAC
    TTCAAGCTGG GCACCATCCG AGGCCAGTTC AACTTCGTCC ACGTGATCAT CACCCCCCTG
    GACTATGAGT GCAACCTGGT GTCCCTGCAG TGCAGGAAAG ACATGGAAGG TCTTGTGGAT
    ACTAGCGTGG CCAAGATCGT GTCTGACCGC AACTTGCCTT TTGTGGCACG TCAGATGGCT
    TTGCATGCAA ATATGGCCTC ACAAGTGCAT CATAGCCGCT CCAACCCCAC CGACATCTAT
    CCCTCCAAGT GGATTGCCAG GCTTCGCCAC ATCAAGCGAC TCCGCCACCG GATCCGCGAG
    GAAGCCCACT ACACCAACCC CAGCCTGCCA CTGATGCAGA TGCACCCCCC GGGCCACCCC
    AAAGCCTCTG CTCAGGCGCC GGCAGAGCCT GTGCCCACGT ATGAGACAGG CCAGCGGAAG
    CGTCTCATCT CCTCTGTGGA TGACTTCACG GAGTTTGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022275959.1
    CDS131..4972
    Translation

    Target ORF information:

    RefSeq Version XM_022420251.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022420251.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG 
    GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC
    GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG
    CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG
    GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG
    ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG
    CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC
    TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC
    CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC
    AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC
    CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG
    TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC
    TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT
    GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA
    GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT
    CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG
    AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC
    AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT
    AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG
    CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC
    AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT
    GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG
    CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG
    GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG
    GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG
    CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC
    GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG
    GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC
    GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC
    ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC
    GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG
    TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC
    CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC
    CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC
    AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT
    CCACCCGTGA AAGAATTCAA AGAGAGCTCT GCCGCCGAGG CCTTCCGATG CCGCAGCATC
    AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATCCAGACGT CCCTCACAAG CGCCAGCTTG
    GGGTCTGCTG ACGAGAACTC GATGGCCCAG GCTGATGACA ACTTGAAAAA TCTCCACCTG
    GAGCTCACGG AAACATGTCT GGACATGATG GCCAGATATG TGTTCTCCAA CTTCACAGCA
    GTCCCCAAGA GGTCCCCGGT GGGAGAGTTC CTCCTGGCTG GTGGCAGGAC CAAAACCTGG
    CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACGAGCGTGG GGACGGGGAC CCGGTCGCTG
    CTGGGCTTGG ACTCAGGAGA TCAGCAAGGT GGCCCCGAGT TGAGCTCGGA CCCTGGCATG
    CATGTGAGAC AGGCAAAGGA GGCACCTGCC AAGCTGGAGT CGCAGGCTGG GCAGCAGGTG
    TGCCGCGGCG CCCGGGACCG GGTCCGCTCC ATGTCCGGGG GCCACGGTCT TCGTGTTGGC
    GCCCTGGACA CTCCAGCCTC CCACTTGCCG GGTGGCCCCA CTTCCCCAGG CACGCAGGCA
    GCGCTGGCCA GCAAGTCTGA CAGGGCCTCG GCTGGCACCC GGTTCCCAGC ACAGGAGAAG
    ACGAGCCTGG CAGCCTACGT ACCGCTGCTG ACCCAGGGCT GGGCGGAGAT CTTGGTCCGG
    AGGCCTACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCCTTCTCG
    TCGGACATCA ACAACATGCC TCTGCAGGAG CTGTCCAACG CGCTCATGGC CGCTGAGCGC
    TTCAAGGAGC GTCGTGACAC GGCTCTGTAC AAGTCGCTGT CGGTGCCTGC CGCAGGCTCG
    GCCAAGCCCC CTCCGCCCCC GAGATCTAAC ACGGTGGCCT CTTTCTCCTC CCTGTACCAG
    TCCAGTTGCC AAGGAAAGCT GCACAGGAGC ATTTCCTGGG CAGACTCTGC GGTGGTTCTG
    GAGGAGGGAA ATTCAAGCGA GACCAGTCTG CCAGTGGAGC ACACGGAGCT GGAGGACTTC
    GAGGCGACAC TAGGCACAGA CCGGCGCTGT GGCCGCGCGG AAGCTTACAG CAGGTCGTCT
    TCAAGCTCCA GCCAGGAGGA GAAGTTCCCT GCAGAGGAGC TGGCTGCCGG AGGGATCCCC
    ATCGAACGAG CTGTCTCTTC CGAGGGTGCC CGGCCTTCTG TGGAGCTCTC CTTCCAGCCC
    TCCCAGCCCC TCAGCAAGTC CAGCTCCTCC CCTGAGCTGC AGACCCTGCA GGACATACTC
    GGGGACCCCG GGGACAAGTC TGATGTTGGC CGGCTCAGCC CTGAGGCCAA GGCCCGGTCA
    CAGTCCGGGA TTCTGGACGG GGTGGGTGCC TCCTGGTCAG CCCCAGGTGA AGAGAACCAG
    GGCCAGGGCC CCGCACAGCC CGAGGGTCCC TTGCCTTCCA GCTGCCCCCG CTCCCCCAGT
    GGCCTGCGGC CCAGAGGCTA CACCATCTCT GATTCAGCCC CGTCACGCAG AGGCAAGAGG
    GTAGAGCGGG ACGCCTTCAG GAGCAGAGCT GGGGCTTCCA ACACTGAGAA GGTGCCAGGC
    ATCAATCCCA GCTTTGTGTT CCTGCAGCTC TACCACTCAC CCTTCTTTGG TGACGAGTCC
    AACAAGCCCA TCCTTCTACC CAATGAGTCC TTCGAGCGGT CAGTGCAGCT TCTCGACCAG
    ATTCCATCAT ACGACACACA CAAGATTGCC GTCCTGTACG TGGGAGAAGG CCAGAGCAGC
    AGTGAGCTCG CCATCCTGTC CAATGAGCAT GGCTCGTACA GGTACACGGA GTTCCTGACA
    GGCCTGGGCA AGCTCATTGA GCTCAAAGAC TGCCAGCCGG ACAAGGTGTA CCTGGGGGGC
    CTGGACGTGT GTGGCGAAGA TGGCCAGTTC ACCTACTGCT GGCATGACGA CATCATGCAA
    GCCGTCTTCC ACATTGCTAC CCTGATGCCC ACCAAGGAAG TGGACAAGCA CCGCTGTGAC
    AAGAAGCGCC ACCTGGGCAA TGACTTTGTG TCCATCGTCT ACAACGACTC TGGTGAGGAC
    TTCAAGCTGG GCACCATCCG AGGCCAGTTC AACTTCGTCC ACGTGATCAT CACCCCCCTG
    GACTATGAGT GCAACCTGGT GTCCCTGCAG TGCAGGAAAG ACATGGAAGG TCTTGTGGAT
    ACTAGCGTGG CCAAGATCGT GTCTGACCGC AACTTGCCTT TTGTGGCACG TCAGATGGCT
    TTGCATGCAA ATATGGCCTC ACAAGTGCAT CATAGCCGCT CCAACCCCAC CGACATCTAT
    CCCTCCAAGT GGATTGCCAG GCTTCGCCAC ATCAAGCGAC TCCGCCACCG GATCCGCGAG
    GAAGCCCACT ACACCAACCC CAGCCTGCCA CTGATGCAGA TGCACCCCCC GGGCCACCCC
    AAAGCCTCTG CTCAGGCGCC GGCAGAGCCT GTGCCCACGT ATGAGACAGG CCAGCGGAAG
    CGTCTCATCT CCTCTGTGGA TGACTTCACG GAGTTTGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe289105
    Clone ID Related Accession (Same CDS sequence) XM_022420252.1
    Accession Version XM_022420252.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4665bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product tuberin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCGGAACC TGCTGGGCAC CCACCTGGGC CACAGTGCCA TCTACAACAT GTGTCGCATC 
    ATGGAGGACA GAGCCTACAT GGAAGATGCC CCACTGCTGA GAGGGGCTGT GTTCTTTGTG
    GGGATGGCCC TTTGGGGAGC ACACCGACTC TACTCTCTCA AGAACTCCCC AACGTCTGTG
    TTGCCGTCGT TTTATGAGGC TATGACCTGT CCGAATGAAG TGGTGTCCTA TGAAATCGTC
    CTCTCTATAA CCAGACTCAT CAAGAAGTAC CGGAGGGAGC TGCAAGCTGT GACCTGGGAC
    ATCCTACTGA ACATCATGGA GCGCCTCCTG CACCAGCTCC AGAGCCTGGA CAGCCCGGAG
    CTCAGAGCCA TCGTGCACGA CCTGCTGACC ACAGTGGAAG AGCTGTGTGA TCAGAATGAG
    TTCCACGGCT CTCAGGAGAG ATACTTTGAG CTGGTTGAGC GATGTGCAGA CCAGAGGCCG
    GAGTCTTCCC TCTTAAACTT AATAACGTAC CGGGCTCAGT CAATCCACCC GGCGAAGGAT
    GGCTGGATTC AGAACCTGCA GTTACTGATG GAGAGGTTCT TCAGGAACGA ATCTCGCAGT
    GCTGTGCGCA TCAAGGTGCT GGACGTCCTG TCCTTCGTGC TGCTCATCAA CAGGCAGTTC
    TATGAGGAGG AGCTGATCAG CTCGGTGGTC ATCTCACAGC TCTCCCATGT CCCGGAGGAC
    AAAGACCCCC AAGTCCGGAA ACTGGCCACC CAGTTGCTGG TGGACCTGGC CGAAGGCTGC
    CACACCCACC ACTTCAACAG TCTGTTGGAC ATTGTGGAAA AGGTGATTGC CCGCTCTCTC
    TCTCCACCCC CTGAGCTGGA GGAAAGAGAC GTGGCGGTGT ACTCGGCCTC CTTAGAGGAT
    GTGAAGACTG CTGTCCTGGG GCTCCTGGTC ATCCTTCAGA CCAAGCTGTA CGCCTTGCCT
    GCCAGCCATG CGATGCGCGT GTACGAGACA CTCGTCAGCC ACATCCAGCT CCACTACAAG
    CATAGCTATA CCCTGCCCAT TGCCAGTAGC ATCCGGCTGC AGGCTTTTGA CTTTCTACTG
    CTGCTGAGGG CCGACTCGCT GCACCGCCTC GGCCTGCCCA GCAAGGACGG GGCCGTGAGG
    TTCAGCCCTT ACTGCGTCTG CGATGCCATG GAGCCAGAGA GAGGCTCAGA GAAGAAGGCC
    AGTGGCCCCC TGTCACCTCC CACGGGGCCT CCTGGTCCTG CGCCCGCAGG CCCCGCCGTG
    CGGCTTGGCT CCCTGCCCTA CTCCCTGCTC TTCCGTGTCC TGCTGCAGTG CCTGAAGCTG
    GAGGTGGACT GGAAGGTACT GAAGCTCGTG CTTAGCAAGC TGCCAGAGTC CCTGCGTTAT
    AAGGTGCTCA TCTTCACCTC GCCCTGCAGC GTGGACCAGC TGTCCTCCGC CCTCTGCTCC
    ATGCTCTCAG GTCCCAGAAC TCTGGAGCGG CTCCGAGGCA CCCCGGAAGG GTTCTCCAGA
    ACCGACCTGC ACCTGGCTGT GGTCCCTGTG CTGACGGCGC TCATCTCTTA CCATAACTAC
    CTGGACAAAA CCAGACAGCG GGAGATGGTG TACTGCCTGG AGCAAGGCCT GATCTACCGC
    TGCGCCAGCC AGTGTGTGGT GGCCCTGGCC ATCTGCAGCG TGGAGATGCC TGACATCATC
    ATCAAGGCGC TTCCCGTCCT GGTCGTGAAG CTCACACACA TCTCGGCCAC GGCCAGTATG
    GCCATTCCAC TCCTCGAGTT CCTGTCAACG CTGGCCAGGT TGCCCCACCT GTACAGGAAC
    TTCGCAGCAG AGCAGTACGC GAGTGTGTTT GCCATCTCCC TGCCCTACAC CAATCCCTCC
    AAGTTCAATC AGTACATCGT GTGCCTGGCC CATCATGTCA TAGCCATGTG GTTCATTAGA
    TGCCGCCTGC CCTTCCGGAA GGATTTTGTT CCTTATATCA CTAAGGGCCT GCGCTCCAAC
    GTCCTCCTGT CTTTTGATGA CACCCCCGAG AAGGACAGTT TCAGAGCACG AAGCACCAGT
    CTCAACGAGA GGCCCAAGAG TTTGAGGATA GCCAGACCCC CCAAACAAGG CTTGAATAAC
    TCTCCACCCG TGAAAGAATT CAAAGAGAGC TCTGCCGCCG AGGCCTTCCG ATGCCGCAGC
    ATCAGTGTGT CTGAACATGT GGTCCGCAGC AGGATCCAGA CGTCCCTCAC AAGCGCCAGC
    TTGGGGTCTG CTGACGAGAA CTCGATGGCC CAGGCTGATG ACAACTTGAA AAATCTCCAC
    CTGGAGCTCA CGGAAACATG TCTGGACATG ATGGCCAGAT ATGTGTTCTC CAACTTCACA
    GCAGTCCCCA AGAGGTCCCC GGTGGGAGAG TTCCTCCTGG CTGGTGGCAG GACCAAAACC
    TGGCTGGTTG GGAACAAGCT TGTCACTGTG ACGACGAGCG TGGGGACGGG GACCCGGTCG
    CTGCTGGGCT TGGACTCAGG AGATCAGCAA GGTGGCCCCG AGTTGAGCTC GGACCCTGGC
    ATGCATGTGA GACAGGCAAA GGAGGCACCT GCCAAGCTGG AGTCGCAGGC TGGGCAGCAG
    GTGTGCCGCG GCGCCCGGGA CCGGGTCCGC TCCATGTCCG GGGGCCACGG TCTTCGTGTT
    GGCGCCCTGG ACACTCCAGC CTCCCACTTG CCGGGTGGCC CCACTTCCCC AGGCACGCAG
    GCAGCGCTGG CCAGCAAGTC TGACAGGGCC TCGGCTGGCA CCCGGTTCCC AGCACAGGAG
    AAGACGAGCC TGGCAGCCTA CGTACCGCTG CTGACCCAGG GCTGGGCGGA GATCTTGGTC
    CGGAGGCCTA CAGGGAACAC CAGCTGGCTG ATGAGCCTGG AGAACCCGCT CAGCCCCTTC
    TCGTCGGACA TCAACAACAT GCCTCTGCAG GAGCTGTCCA ACGCGCTCAT GGCCGCTGAG
    CGCTTCAAGG AGCGTCGTGA CACGGCTCTG TACAAGTCGC TGTCGGTGCC TGCCGCAGGC
    TCGGCCAAGC CCCCTCCGCC CCCGAGATCT AACACGGTGG CCTCTTTCTC CTCCCTGTAC
    CAGTCCAGTT GCCAAGGAAA GCTGCACAGG AGCATTTCCT GGGCAGACTC TGCGGTGGTT
    CTGGAGGAGG GAAATTCAAG CGAGACCAGT CTGCCAGTGG AGCACACGGA GCTGGAGGAC
    TTCGAGGCGA CACTAGGCAC AGACCGGCGC TGTGGCCGCG CGGAAGCTTA CAGCAGGTCG
    TCTTCAAGCT CCAGCCAGGA GGAGAAGTTC CCTGCAGAGG AGCTGGCTGC CGGAGGGATC
    CCCATCGAAC GAGCTGTCTC TTCCGAGGGT GCCCGGCCTT CTGTGGAGCT CTCCTTCCAG
    CCCTCCCAGC CCCTCAGCAA GTCCAGCTCC TCCCCTGAGC TGCAGACCCT GCAGGACATA
    CTCGGGGACC CCGGGGACAA GTCTGATGTT GGCCGGCTCA GCCCTGAGGC CAAGGCCCGG
    TCACAGTCCG GGATTCTGGA CGGGGTGGGT GCCTCCTGGT CAGCCCCAGG TGAAGAGAAC
    CAGGGCCAGG GCCCCGCACA GCCCGAGGGT CCCTTGCCTT CCAGCTGCCC CCGCTCCCCC
    AGTGGCCTGC GGCCCAGAGG CTACACCATC TCTGATTCAG CCCCGTCACG CAGAGGCAAG
    AGGGTAGAGC GGGACGCCTT CAGGAGCAGA GCTGGGGCTT CCAACACTGA GAAGGTGCCA
    GGCATCAATC CCAGCTTTGT GTTCCTGCAG CTCTACCACT CACCCTTCTT TGGTGACGAG
    TCCAACAAGC CCATCCTTCT ACCCAATGAG TCCTTCGAGC GGTCAGTGCA GCTTCTCGAC
    CAGATTCCAT CATACGACAC ACACAAGATT GCCGTCCTGT ACGTGGGAGA AGGCCAGAGC
    AGCAGTGAGC TCGCCATCCT GTCCAATGAG CATGGCTCGT ACAGGTACAC GGAGTTCCTG
    ACAGGCCTGG GCAAGCTCAT TGAGCTCAAA GACTGCCAGC CGGACAAGGT GTACCTGGGG
    GGCCTGGACG TGTGTGGCGA AGATGGCCAG TTCACCTACT GCTGGCATGA CGACATCATG
    CAAGCCGTCT TCCACATTGC TACCCTGATG CCCACCAAGG AAGTGGACAA GCACCGCTGT
    GACAAGAAGC GCCACCTGGG CAATGACTTT GTGTCCATCG TCTACAACGA CTCTGGTGAG
    GACTTCAAGC TGGGCACCAT CCGAGGCCAG TTCAACTTCG TCCACGTGAT CATCACCCCC
    CTGGACTATG AGTGCAACCT GGTGTCCCTG CAGTGCAGGA AAGACATGGA AGGTCTTGTG
    GATACTAGCG TGGCCAAGAT CGTGTCTGAC CGCAACTTGC CTTTTGTGGC ACGTCAGATG
    GCTTTGCATG CAAATATGGC CTCACAAGTG CATCATAGCC GCTCCAACCC CACCGACATC
    TATCCCTCCA AGTGGATTGC CAGGCTTCGC CACATCAAGC GACTCCGCCA CCGGATCCGC
    GAGGAAGCCC ACTACACCAA CCCCAGCCTG CCACTGATGC AGATGCACCC CCCGGGCCAC
    CCCAAAGCCT CTGCTCAGGC GCCGGCAGAG CCTGTGCCCA CGTATGAGAC AGGCCAGCGG
    AAGCGTCTCA TCTCCTCTGT GGATGACTTC ACGGAGTTTG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022275960.1
    CDS113..4777
    Translation

    Target ORF information:

    RefSeq Version XM_022420252.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022420252.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCGGAACC TGCTGGGCAC CCACCTGGGC CACAGTGCCA TCTACAACAT GTGTCGCATC 
    ATGGAGGACA GAGCCTACAT GGAAGATGCC CCACTGCTGA GAGGGGCTGT GTTCTTTGTG
    GGGATGGCCC TTTGGGGAGC ACACCGACTC TACTCTCTCA AGAACTCCCC AACGTCTGTG
    TTGCCGTCGT TTTATGAGGC TATGACCTGT CCGAATGAAG TGGTGTCCTA TGAAATCGTC
    CTCTCTATAA CCAGACTCAT CAAGAAGTAC CGGAGGGAGC TGCAAGCTGT GACCTGGGAC
    ATCCTACTGA ACATCATGGA GCGCCTCCTG CACCAGCTCC AGAGCCTGGA CAGCCCGGAG
    CTCAGAGCCA TCGTGCACGA CCTGCTGACC ACAGTGGAAG AGCTGTGTGA TCAGAATGAG
    TTCCACGGCT CTCAGGAGAG ATACTTTGAG CTGGTTGAGC GATGTGCAGA CCAGAGGCCG
    GAGTCTTCCC TCTTAAACTT AATAACGTAC CGGGCTCAGT CAATCCACCC GGCGAAGGAT
    GGCTGGATTC AGAACCTGCA GTTACTGATG GAGAGGTTCT TCAGGAACGA ATCTCGCAGT
    GCTGTGCGCA TCAAGGTGCT GGACGTCCTG TCCTTCGTGC TGCTCATCAA CAGGCAGTTC
    TATGAGGAGG AGCTGATCAG CTCGGTGGTC ATCTCACAGC TCTCCCATGT CCCGGAGGAC
    AAAGACCCCC AAGTCCGGAA ACTGGCCACC CAGTTGCTGG TGGACCTGGC CGAAGGCTGC
    CACACCCACC ACTTCAACAG TCTGTTGGAC ATTGTGGAAA AGGTGATTGC CCGCTCTCTC
    TCTCCACCCC CTGAGCTGGA GGAAAGAGAC GTGGCGGTGT ACTCGGCCTC CTTAGAGGAT
    GTGAAGACTG CTGTCCTGGG GCTCCTGGTC ATCCTTCAGA CCAAGCTGTA CGCCTTGCCT
    GCCAGCCATG CGATGCGCGT GTACGAGACA CTCGTCAGCC ACATCCAGCT CCACTACAAG
    CATAGCTATA CCCTGCCCAT TGCCAGTAGC ATCCGGCTGC AGGCTTTTGA CTTTCTACTG
    CTGCTGAGGG CCGACTCGCT GCACCGCCTC GGCCTGCCCA GCAAGGACGG GGCCGTGAGG
    TTCAGCCCTT ACTGCGTCTG CGATGCCATG GAGCCAGAGA GAGGCTCAGA GAAGAAGGCC
    AGTGGCCCCC TGTCACCTCC CACGGGGCCT CCTGGTCCTG CGCCCGCAGG CCCCGCCGTG
    CGGCTTGGCT CCCTGCCCTA CTCCCTGCTC TTCCGTGTCC TGCTGCAGTG CCTGAAGCTG
    GAGGTGGACT GGAAGGTACT GAAGCTCGTG CTTAGCAAGC TGCCAGAGTC CCTGCGTTAT
    AAGGTGCTCA TCTTCACCTC GCCCTGCAGC GTGGACCAGC TGTCCTCCGC CCTCTGCTCC
    ATGCTCTCAG GTCCCAGAAC TCTGGAGCGG CTCCGAGGCA CCCCGGAAGG GTTCTCCAGA
    ACCGACCTGC ACCTGGCTGT GGTCCCTGTG CTGACGGCGC TCATCTCTTA CCATAACTAC
    CTGGACAAAA CCAGACAGCG GGAGATGGTG TACTGCCTGG AGCAAGGCCT GATCTACCGC
    TGCGCCAGCC AGTGTGTGGT GGCCCTGGCC ATCTGCAGCG TGGAGATGCC TGACATCATC
    ATCAAGGCGC TTCCCGTCCT GGTCGTGAAG CTCACACACA TCTCGGCCAC GGCCAGTATG
    GCCATTCCAC TCCTCGAGTT CCTGTCAACG CTGGCCAGGT TGCCCCACCT GTACAGGAAC
    TTCGCAGCAG AGCAGTACGC GAGTGTGTTT GCCATCTCCC TGCCCTACAC CAATCCCTCC
    AAGTTCAATC AGTACATCGT GTGCCTGGCC CATCATGTCA TAGCCATGTG GTTCATTAGA
    TGCCGCCTGC CCTTCCGGAA GGATTTTGTT CCTTATATCA CTAAGGGCCT GCGCTCCAAC
    GTCCTCCTGT CTTTTGATGA CACCCCCGAG AAGGACAGTT TCAGAGCACG AAGCACCAGT
    CTCAACGAGA GGCCCAAGAG TTTGAGGATA GCCAGACCCC CCAAACAAGG CTTGAATAAC
    TCTCCACCCG TGAAAGAATT CAAAGAGAGC TCTGCCGCCG AGGCCTTCCG ATGCCGCAGC
    ATCAGTGTGT CTGAACATGT GGTCCGCAGC AGGATCCAGA CGTCCCTCAC AAGCGCCAGC
    TTGGGGTCTG CTGACGAGAA CTCGATGGCC CAGGCTGATG ACAACTTGAA AAATCTCCAC
    CTGGAGCTCA CGGAAACATG TCTGGACATG ATGGCCAGAT ATGTGTTCTC CAACTTCACA
    GCAGTCCCCA AGAGGTCCCC GGTGGGAGAG TTCCTCCTGG CTGGTGGCAG GACCAAAACC
    TGGCTGGTTG GGAACAAGCT TGTCACTGTG ACGACGAGCG TGGGGACGGG GACCCGGTCG
    CTGCTGGGCT TGGACTCAGG AGATCAGCAA GGTGGCCCCG AGTTGAGCTC GGACCCTGGC
    ATGCATGTGA GACAGGCAAA GGAGGCACCT GCCAAGCTGG AGTCGCAGGC TGGGCAGCAG
    GTGTGCCGCG GCGCCCGGGA CCGGGTCCGC TCCATGTCCG GGGGCCACGG TCTTCGTGTT
    GGCGCCCTGG ACACTCCAGC CTCCCACTTG CCGGGTGGCC CCACTTCCCC AGGCACGCAG
    GCAGCGCTGG CCAGCAAGTC TGACAGGGCC TCGGCTGGCA CCCGGTTCCC AGCACAGGAG
    AAGACGAGCC TGGCAGCCTA CGTACCGCTG CTGACCCAGG GCTGGGCGGA GATCTTGGTC
    CGGAGGCCTA CAGGGAACAC CAGCTGGCTG ATGAGCCTGG AGAACCCGCT CAGCCCCTTC
    TCGTCGGACA TCAACAACAT GCCTCTGCAG GAGCTGTCCA ACGCGCTCAT GGCCGCTGAG
    CGCTTCAAGG AGCGTCGTGA CACGGCTCTG TACAAGTCGC TGTCGGTGCC TGCCGCAGGC
    TCGGCCAAGC CCCCTCCGCC CCCGAGATCT AACACGGTGG CCTCTTTCTC CTCCCTGTAC
    CAGTCCAGTT GCCAAGGAAA GCTGCACAGG AGCATTTCCT GGGCAGACTC TGCGGTGGTT
    CTGGAGGAGG GAAATTCAAG CGAGACCAGT CTGCCAGTGG AGCACACGGA GCTGGAGGAC
    TTCGAGGCGA CACTAGGCAC AGACCGGCGC TGTGGCCGCG CGGAAGCTTA CAGCAGGTCG
    TCTTCAAGCT CCAGCCAGGA GGAGAAGTTC CCTGCAGAGG AGCTGGCTGC CGGAGGGATC
    CCCATCGAAC GAGCTGTCTC TTCCGAGGGT GCCCGGCCTT CTGTGGAGCT CTCCTTCCAG
    CCCTCCCAGC CCCTCAGCAA GTCCAGCTCC TCCCCTGAGC TGCAGACCCT GCAGGACATA
    CTCGGGGACC CCGGGGACAA GTCTGATGTT GGCCGGCTCA GCCCTGAGGC CAAGGCCCGG
    TCACAGTCCG GGATTCTGGA CGGGGTGGGT GCCTCCTGGT CAGCCCCAGG TGAAGAGAAC
    CAGGGCCAGG GCCCCGCACA GCCCGAGGGT CCCTTGCCTT CCAGCTGCCC CCGCTCCCCC
    AGTGGCCTGC GGCCCAGAGG CTACACCATC TCTGATTCAG CCCCGTCACG CAGAGGCAAG
    AGGGTAGAGC GGGACGCCTT CAGGAGCAGA GCTGGGGCTT CCAACACTGA GAAGGTGCCA
    GGCATCAATC CCAGCTTTGT GTTCCTGCAG CTCTACCACT CACCCTTCTT TGGTGACGAG
    TCCAACAAGC CCATCCTTCT ACCCAATGAG TCCTTCGAGC GGTCAGTGCA GCTTCTCGAC
    CAGATTCCAT CATACGACAC ACACAAGATT GCCGTCCTGT ACGTGGGAGA AGGCCAGAGC
    AGCAGTGAGC TCGCCATCCT GTCCAATGAG CATGGCTCGT ACAGGTACAC GGAGTTCCTG
    ACAGGCCTGG GCAAGCTCAT TGAGCTCAAA GACTGCCAGC CGGACAAGGT GTACCTGGGG
    GGCCTGGACG TGTGTGGCGA AGATGGCCAG TTCACCTACT GCTGGCATGA CGACATCATG
    CAAGCCGTCT TCCACATTGC TACCCTGATG CCCACCAAGG AAGTGGACAA GCACCGCTGT
    GACAAGAAGC GCCACCTGGG CAATGACTTT GTGTCCATCG TCTACAACGA CTCTGGTGAG
    GACTTCAAGC TGGGCACCAT CCGAGGCCAG TTCAACTTCG TCCACGTGAT CATCACCCCC
    CTGGACTATG AGTGCAACCT GGTGTCCCTG CAGTGCAGGA AAGACATGGA AGGTCTTGTG
    GATACTAGCG TGGCCAAGAT CGTGTCTGAC CGCAACTTGC CTTTTGTGGC ACGTCAGATG
    GCTTTGCATG CAAATATGGC CTCACAAGTG CATCATAGCC GCTCCAACCC CACCGACATC
    TATCCCTCCA AGTGGATTGC CAGGCTTCGC CACATCAAGC GACTCCGCCA CCGGATCCGC
    GAGGAAGCCC ACTACACCAA CCCCAGCCTG CCACTGATGC AGATGCACCC CCCGGGCCAC
    CCCAAAGCCT CTGCTCAGGC GCCGGCAGAG CCTGTGCCCA CGTATGAGAC AGGCCAGCGG
    AAGCGTCTCA TCTCCTCTGT GGATGACTTC ACGGAGTTTG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe37159
    Clone ID Related Accession (Same CDS sequence) XM_014114692.1 , XM_014114692.2
    Accession Version XM_014114692.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5241bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product tuberin isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
    CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC
    ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG
    ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG
    CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA
    GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG
    GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG
    GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG
    GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT
    GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC
    ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC
    GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG
    CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG
    GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG
    ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG
    CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC
    TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC
    CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC
    AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC
    CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG
    TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC
    TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT
    GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA
    GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT
    CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG
    AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC
    AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT
    AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG
    CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC
    AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT
    GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG
    CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG
    GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG
    GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG
    CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC
    GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG
    GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC
    GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC
    ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC
    GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG
    TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC
    CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC
    CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC
    AACGAGAGGC CCAAGAGGAT CCAGACGTCC CTCACAAGCG CCAGCTTGGG GTCTGCTGAC
    GAGAACTCGA TGGCCCAGGC TGATGACAAC TTGAAAAATC TCCACCTGGA GCTCACGGAA
    ACATGTCTGG ACATGATGGC CAGATATGTG TTCTCCAACT TCACAGCAGT CCCCAAGAGG
    TCCCCGGTGG GAGAGTTCCT CCTGGCTGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC
    AAGCTTGTCA CTGTGACGAC GAGCGTGGGG ACGGGGACCC GGTCGCTGCT GGGCTTGGAC
    TCAGGAGATC AGCAAGGTGG CCCCGAGTTG AGCTCGGACC CTGGCATGCA TGTGAGACAG
    GCAAAGGAGG CACCTGCCAA GCTGGAGTCG CAGGCTGGGC AGCAGGTGTG CCGCGGCGCC
    CGGGACCGGG TCCGCTCCAT GTCCGGGGGC CACGGTCTTC GTGTTGGCGC CCTGGACACT
    CCAGCCTCCC ACTTGCCGGG TGGCCCCACT TCCCCAGGCA CGCAGGCAGC GCTGGCCAGC
    AAGTCTGACA GGGCCTCGGC TGGCACCCGG TTCCCAGCAC AGGAGAAGAC GAGCCTGGCA
    GCCTACGTAC CGCTGCTGAC CCAGGGCTGG GCGGAGATCT TGGTCCGGAG GCCTACAGGG
    AACACCAGCT GGCTGATGAG CCTGGAGAAC CCGCTCAGCC CCTTCTCGTC GGACATCAAC
    AACATGCCTC TGCAGGAGCT GTCCAACGCG CTCATGGCCG CTGAGCGCTT CAAGGAGCGT
    CGTGACACGG CTCTGTACAA GTCGCTGTCG GTGCCTGCCG CAGGCTCGGC CAAGCCCCCT
    CCGCCCCCGA GATCTAACAC GGACTCTGCG GTGGTTCTGG AGGAGGGAAA TTCAAGCGAG
    ACCAGTCTGC CAGTGGAGCA CACGGAGCTG GAGGACTTCG AGGCGACACT AGGCACAGAC
    CGGCGCTGTG GCCGCGCGGA AGCTTACAGC AGGTCGTCTT CAAGCTCCAG CCAGGAGGAG
    AAGTTCCCTG CAGAGGAGCT GGCTGCCGGA GGGATCCCCA TCGAACGAGC TGTCTCTTCC
    GAGGGTGCCC GGCCTTCTGT GGAGCTCTCC TTCCAGCCCT CCCAGCCCCT CAGCAAGTCC
    AGCTCCTCCC CTGAGCTGCA GACCCTGCAG GACATACTCG GGGACCCCGG GGACAAGTCT
    GATGTTGGCC GGCTCAGCCC TGAGGCCAAG GCCCGGTCAC AGTCCGGGAT TCTGGACGGG
    GTGGGTGCCT CCTGGTCAGC CCCAGGTGAA GAGAACCAGG GCCAGGGCCC CGCACAGCCC
    GAGGGTCCCT TGCCTTCCAG CTGCCCCCGC TCCCCCAGTG GCCTGCGGCC CAGAGGCTAC
    ACCATCTCTG ATTCAGCCCC GTCACGCAGA GGCAAGAGGG TAGAGCGGGA CGCCTTCAGG
    AGCAGAGCTG GGGCTTCCAA CACTGAGAAG GTGCCAGGCA TCAATCCCAG CTTTGTGTTC
    CTGCAGCTCT ACCACTCACC CTTCTTTGGT GACGAGTCCA ACAAGCCCAT CCTTCTACCC
    AATGAGTCCT TCGAGCGGTC AGTGCAGCTT CTCGACCAGA TTCCATCATA CGACACACAC
    AAGATTGCCG TCCTGTACGT GGGAGAAGGC CAGAGCAGCA GTGAGCTCGC CATCCTGTCC
    AATGAGCATG GCTCGTACAG GTACACGGAG TTCCTGACAG GCCTGGGCAA GCTCATTGAG
    CTCAAAGACT GCCAGCCGGA CAAGGTGTAC CTGGGGGGCC TGGACGTGTG TGGCGAAGAT
    GGCCAGTTCA CCTACTGCTG GCATGACGAC ATCATGCAAG CCGTCTTCCA CATTGCTACC
    CTGATGCCCA CCAAGGAAGT GGACAAGCAC CGCTGTGACA AGAAGCGCCA CCTGGGCAAT
    GACTTTGTGT CCATCGTCTA CAACGACTCT GGTGAGGACT TCAAGCTGGG CACCATCCGA
    GGCCAGTTCA ACTTCGTCCA CGTGATCATC ACCCCCCTGG ACTATGAGTG CAACCTGGTG
    TCCCTGCAGT GCAGGAAAGA CATGGAAGGT CTTGTGGATA CTAGCGTGGC CAAGATCGTG
    TCTGACCGCA ACTTGCCTTT TGTGGCACGT CAGATGGCTT TGCATGCAAA TATGGCCTCA
    CAAGTGCATC ATAGCCGCTC CAACCCCACC GACATCTATC CCTCCAAGTG GATTGCCAGG
    CTTCGCCACA TCAAGCGACT CCGCCACCGG ATCCGCGAGG AAGCCCACTA CACCAACCCC
    AGCCTGCCAC TGATGCAGAT GCACCCCCCG GGCCACCCCA AAGCCTCTGC TCAGGCGCCG
    GCAGAGCCTG TGCCCACGTA TGAGACAGGC CAGCGGAAGC GTCTCATCTC CTCTGTGGAT
    GACTTCACGG AGTTTGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013970167.1
    CDS282..5522
    Translation

    Target ORF information:

    RefSeq Version XM_014114692.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris tuberous sclerosis 2 (TSC2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014114692.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
    CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC
    ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG
    ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG
    CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA
    GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG
    GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG
    GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG
    GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT
    GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC
    ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC
    GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG
    CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG
    GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG
    ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG
    CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC
    TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC
    CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC
    AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC
    CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG
    TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC
    TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT
    GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA
    GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT
    CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG
    AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC
    AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT
    AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG
    CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC
    AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT
    GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG
    CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG
    GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG
    GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG
    CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC
    GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG
    GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC
    GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC
    ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC
    GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG
    TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC
    CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC
    CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC
    AACGAGAGGC CCAAGAGGAT CCAGACGTCC CTCACAAGCG CCAGCTTGGG GTCTGCTGAC
    GAGAACTCGA TGGCCCAGGC TGATGACAAC TTGAAAAATC TCCACCTGGA GCTCACGGAA
    ACATGTCTGG ACATGATGGC CAGATATGTG TTCTCCAACT TCACAGCAGT CCCCAAGAGG
    TCCCCGGTGG GAGAGTTCCT CCTGGCTGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC
    AAGCTTGTCA CTGTGACGAC GAGCGTGGGG ACGGGGACCC GGTCGCTGCT GGGCTTGGAC
    TCAGGAGATC AGCAAGGTGG CCCCGAGTTG AGCTCGGACC CTGGCATGCA TGTGAGACAG
    GCAAAGGAGG CACCTGCCAA GCTGGAGTCG CAGGCTGGGC AGCAGGTGTG CCGCGGCGCC
    CGGGACCGGG TCCGCTCCAT GTCCGGGGGC CACGGTCTTC GTGTTGGCGC CCTGGACACT
    CCAGCCTCCC ACTTGCCGGG TGGCCCCACT TCCCCAGGCA CGCAGGCAGC GCTGGCCAGC
    AAGTCTGACA GGGCCTCGGC TGGCACCCGG TTCCCAGCAC AGGAGAAGAC GAGCCTGGCA
    GCCTACGTAC CGCTGCTGAC CCAGGGCTGG GCGGAGATCT TGGTCCGGAG GCCTACAGGG
    AACACCAGCT GGCTGATGAG CCTGGAGAAC CCGCTCAGCC CCTTCTCGTC GGACATCAAC
    AACATGCCTC TGCAGGAGCT GTCCAACGCG CTCATGGCCG CTGAGCGCTT CAAGGAGCGT
    CGTGACACGG CTCTGTACAA GTCGCTGTCG GTGCCTGCCG CAGGCTCGGC CAAGCCCCCT
    CCGCCCCCGA GATCTAACAC GGACTCTGCG GTGGTTCTGG AGGAGGGAAA TTCAAGCGAG
    ACCAGTCTGC CAGTGGAGCA CACGGAGCTG GAGGACTTCG AGGCGACACT AGGCACAGAC
    CGGCGCTGTG GCCGCGCGGA AGCTTACAGC AGGTCGTCTT CAAGCTCCAG CCAGGAGGAG
    AAGTTCCCTG CAGAGGAGCT GGCTGCCGGA GGGATCCCCA TCGAACGAGC TGTCTCTTCC
    GAGGGTGCCC GGCCTTCTGT GGAGCTCTCC TTCCAGCCCT CCCAGCCCCT CAGCAAGTCC
    AGCTCCTCCC CTGAGCTGCA GACCCTGCAG GACATACTCG GGGACCCCGG GGACAAGTCT
    GATGTTGGCC GGCTCAGCCC TGAGGCCAAG GCCCGGTCAC AGTCCGGGAT TCTGGACGGG
    GTGGGTGCCT CCTGGTCAGC CCCAGGTGAA GAGAACCAGG GCCAGGGCCC CGCACAGCCC
    GAGGGTCCCT TGCCTTCCAG CTGCCCCCGC TCCCCCAGTG GCCTGCGGCC CAGAGGCTAC
    ACCATCTCTG ATTCAGCCCC GTCACGCAGA GGCAAGAGGG TAGAGCGGGA CGCCTTCAGG
    AGCAGAGCTG GGGCTTCCAA CACTGAGAAG GTGCCAGGCA TCAATCCCAG CTTTGTGTTC
    CTGCAGCTCT ACCACTCACC CTTCTTTGGT GACGAGTCCA ACAAGCCCAT CCTTCTACCC
    AATGAGTCCT TCGAGCGGTC AGTGCAGCTT CTCGACCAGA TTCCATCATA CGACACACAC
    AAGATTGCCG TCCTGTACGT GGGAGAAGGC CAGAGCAGCA GTGAGCTCGC CATCCTGTCC
    AATGAGCATG GCTCGTACAG GTACACGGAG TTCCTGACAG GCCTGGGCAA GCTCATTGAG
    CTCAAAGACT GCCAGCCGGA CAAGGTGTAC CTGGGGGGCC TGGACGTGTG TGGCGAAGAT
    GGCCAGTTCA CCTACTGCTG GCATGACGAC ATCATGCAAG CCGTCTTCCA CATTGCTACC
    CTGATGCCCA CCAAGGAAGT GGACAAGCAC CGCTGTGACA AGAAGCGCCA CCTGGGCAAT
    GACTTTGTGT CCATCGTCTA CAACGACTCT GGTGAGGACT TCAAGCTGGG CACCATCCGA
    GGCCAGTTCA ACTTCGTCCA CGTGATCATC ACCCCCCTGG ACTATGAGTG CAACCTGGTG
    TCCCTGCAGT GCAGGAAAGA CATGGAAGGT CTTGTGGATA CTAGCGTGGC CAAGATCGTG
    TCTGACCGCA ACTTGCCTTT TGTGGCACGT CAGATGGCTT TGCATGCAAA TATGGCCTCA
    CAAGTGCATC ATAGCCGCTC CAACCCCACC GACATCTATC CCTCCAAGTG GATTGCCAGG
    CTTCGCCACA TCAAGCGACT CCGCCACCGG ATCCGCGAGG AAGCCCACTA CACCAACCCC
    AGCCTGCCAC TGATGCAGAT GCACCCCCCG GGCCACCCCA AAGCCTCTGC TCAGGCGCCG
    GCAGAGCCTG TGCCCACGTA TGAGACAGGC CAGCGGAAGC GTCTCATCTC CTCTGTGGAT
    GACTTCACGG AGTTTGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe37159
    Clone ID Related Accession (Same CDS sequence) XM_014114692.1 , XM_014114692.2
    Accession Version XM_014114692.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5241bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product tuberin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014114692.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
    CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC
    ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG
    ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG
    CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA
    GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG
    GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG
    GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG
    GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT
    GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC
    ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC
    GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG
    CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG
    GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG
    ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG
    CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC
    TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC
    CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC
    AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC
    CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG
    TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC
    TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT
    GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA
    GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT
    CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG
    AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC
    AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT
    AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG
    CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC
    AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT
    GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG
    CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG
    GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG
    GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG
    CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC
    GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG
    GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC
    GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC
    ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC
    GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG
    TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC
    CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC
    CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC
    AACGAGAGGC CCAAGAGGAT CCAGACGTCC CTCACAAGCG CCAGCTTGGG GTCTGCTGAC
    GAGAACTCGA TGGCCCAGGC TGATGACAAC TTGAAAAATC TCCACCTGGA GCTCACGGAA
    ACATGTCTGG ACATGATGGC CAGATATGTG TTCTCCAACT TCACAGCAGT CCCCAAGAGG
    TCCCCGGTGG GAGAGTTCCT CCTGGCTGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC
    AAGCTTGTCA CTGTGACGAC GAGCGTGGGG ACGGGGACCC GGTCGCTGCT GGGCTTGGAC
    TCAGGAGATC AGCAAGGTGG CCCCGAGTTG AGCTCGGACC CTGGCATGCA TGTGAGACAG
    GCAAAGGAGG CACCTGCCAA GCTGGAGTCG CAGGCTGGGC AGCAGGTGTG CCGCGGCGCC
    CGGGACCGGG TCCGCTCCAT GTCCGGGGGC CACGGTCTTC GTGTTGGCGC CCTGGACACT
    CCAGCCTCCC ACTTGCCGGG TGGCCCCACT TCCCCAGGCA CGCAGGCAGC GCTGGCCAGC
    AAGTCTGACA GGGCCTCGGC TGGCACCCGG TTCCCAGCAC AGGAGAAGAC GAGCCTGGCA
    GCCTACGTAC CGCTGCTGAC CCAGGGCTGG GCGGAGATCT TGGTCCGGAG GCCTACAGGG
    AACACCAGCT GGCTGATGAG CCTGGAGAAC CCGCTCAGCC CCTTCTCGTC GGACATCAAC
    AACATGCCTC TGCAGGAGCT GTCCAACGCG CTCATGGCCG CTGAGCGCTT CAAGGAGCGT
    CGTGACACGG CTCTGTACAA GTCGCTGTCG GTGCCTGCCG CAGGCTCGGC CAAGCCCCCT
    CCGCCCCCGA GATCTAACAC GGACTCTGCG GTGGTTCTGG AGGAGGGAAA TTCAAGCGAG
    ACCAGTCTGC CAGTGGAGCA CACGGAGCTG GAGGACTTCG AGGCGACACT AGGCACAGAC
    CGGCGCTGTG GCCGCGCGGA AGCTTACAGC AGGTCGTCTT CAAGCTCCAG CCAGGAGGAG
    AAGTTCCCTG CAGAGGAGCT GGCTGCCGGA GGGATCCCCA TCGAACGAGC TGTCTCTTCC
    GAGGGTGCCC GGCCTTCTGT GGAGCTCTCC TTCCAGCCCT CCCAGCCCCT CAGCAAGTCC
    AGCTCCTCCC CTGAGCTGCA GACCCTGCAG GACATACTCG GGGACCCCGG GGACAAGTCT
    GATGTTGGCC GGCTCAGCCC TGAGGCCAAG GCCCGGTCAC AGTCCGGGAT TCTGGACGGG
    GTGGGTGCCT CCTGGTCAGC CCCAGGTGAA GAGAACCAGG GCCAGGGCCC CGCACAGCCC
    GAGGGTCCCT TGCCTTCCAG CTGCCCCCGC TCCCCCAGTG GCCTGCGGCC CAGAGGCTAC
    ACCATCTCTG ATTCAGCCCC GTCACGCAGA GGCAAGAGGG TAGAGCGGGA CGCCTTCAGG
    AGCAGAGCTG GGGCTTCCAA CACTGAGAAG GTGCCAGGCA TCAATCCCAG CTTTGTGTTC
    CTGCAGCTCT ACCACTCACC CTTCTTTGGT GACGAGTCCA ACAAGCCCAT CCTTCTACCC
    AATGAGTCCT TCGAGCGGTC AGTGCAGCTT CTCGACCAGA TTCCATCATA CGACACACAC
    AAGATTGCCG TCCTGTACGT GGGAGAAGGC CAGAGCAGCA GTGAGCTCGC CATCCTGTCC
    AATGAGCATG GCTCGTACAG GTACACGGAG TTCCTGACAG GCCTGGGCAA GCTCATTGAG
    CTCAAAGACT GCCAGCCGGA CAAGGTGTAC CTGGGGGGCC TGGACGTGTG TGGCGAAGAT
    GGCCAGTTCA CCTACTGCTG GCATGACGAC ATCATGCAAG CCGTCTTCCA CATTGCTACC
    CTGATGCCCA CCAAGGAAGT GGACAAGCAC CGCTGTGACA AGAAGCGCCA CCTGGGCAAT
    GACTTTGTGT CCATCGTCTA CAACGACTCT GGTGAGGACT TCAAGCTGGG CACCATCCGA
    GGCCAGTTCA ACTTCGTCCA CGTGATCATC ACCCCCCTGG ACTATGAGTG CAACCTGGTG
    TCCCTGCAGT GCAGGAAAGA CATGGAAGGT CTTGTGGATA CTAGCGTGGC CAAGATCGTG
    TCTGACCGCA ACTTGCCTTT TGTGGCACGT CAGATGGCTT TGCATGCAAA TATGGCCTCA
    CAAGTGCATC ATAGCCGCTC CAACCCCACC GACATCTATC CCTCCAAGTG GATTGCCAGG
    CTTCGCCACA TCAAGCGACT CCGCCACCGG ATCCGCGAGG AAGCCCACTA CACCAACCCC
    AGCCTGCCAC TGATGCAGAT GCACCCCCCG GGCCACCCCA AAGCCTCTGC TCAGGCGCCG
    GCAGAGCCTG TGCCCACGTA TGAGACAGGC CAGCGGAAGC GTCTCATCTC CTCTGTGGAT
    GACTTCACGG AGTTTGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013970167.1
    CDS289..5529
    Translation

    Target ORF information:

    RefSeq Version XM_014114692.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014114692.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
    CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC
    ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG
    ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG
    CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA
    GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG
    GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG
    GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG
    GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT
    GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC
    ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC
    GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG
    CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG
    GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG
    ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG
    CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC
    TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC
    CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC
    AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC
    CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG
    TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC
    TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT
    GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA
    GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT
    CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG
    AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC
    AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT
    AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG
    CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC
    AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT
    GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG
    CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG
    GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG
    GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG
    CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC
    GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG
    GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC
    GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC
    ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC
    GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG
    TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC
    CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC
    CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC
    AACGAGAGGC CCAAGAGGAT CCAGACGTCC CTCACAAGCG CCAGCTTGGG GTCTGCTGAC
    GAGAACTCGA TGGCCCAGGC TGATGACAAC TTGAAAAATC TCCACCTGGA GCTCACGGAA
    ACATGTCTGG ACATGATGGC CAGATATGTG TTCTCCAACT TCACAGCAGT CCCCAAGAGG
    TCCCCGGTGG GAGAGTTCCT CCTGGCTGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC
    AAGCTTGTCA CTGTGACGAC GAGCGTGGGG ACGGGGACCC GGTCGCTGCT GGGCTTGGAC
    TCAGGAGATC AGCAAGGTGG CCCCGAGTTG AGCTCGGACC CTGGCATGCA TGTGAGACAG
    GCAAAGGAGG CACCTGCCAA GCTGGAGTCG CAGGCTGGGC AGCAGGTGTG CCGCGGCGCC
    CGGGACCGGG TCCGCTCCAT GTCCGGGGGC CACGGTCTTC GTGTTGGCGC CCTGGACACT
    CCAGCCTCCC ACTTGCCGGG TGGCCCCACT TCCCCAGGCA CGCAGGCAGC GCTGGCCAGC
    AAGTCTGACA GGGCCTCGGC TGGCACCCGG TTCCCAGCAC AGGAGAAGAC GAGCCTGGCA
    GCCTACGTAC CGCTGCTGAC CCAGGGCTGG GCGGAGATCT TGGTCCGGAG GCCTACAGGG
    AACACCAGCT GGCTGATGAG CCTGGAGAAC CCGCTCAGCC CCTTCTCGTC GGACATCAAC
    AACATGCCTC TGCAGGAGCT GTCCAACGCG CTCATGGCCG CTGAGCGCTT CAAGGAGCGT
    CGTGACACGG CTCTGTACAA GTCGCTGTCG GTGCCTGCCG CAGGCTCGGC CAAGCCCCCT
    CCGCCCCCGA GATCTAACAC GGACTCTGCG GTGGTTCTGG AGGAGGGAAA TTCAAGCGAG
    ACCAGTCTGC CAGTGGAGCA CACGGAGCTG GAGGACTTCG AGGCGACACT AGGCACAGAC
    CGGCGCTGTG GCCGCGCGGA AGCTTACAGC AGGTCGTCTT CAAGCTCCAG CCAGGAGGAG
    AAGTTCCCTG CAGAGGAGCT GGCTGCCGGA GGGATCCCCA TCGAACGAGC TGTCTCTTCC
    GAGGGTGCCC GGCCTTCTGT GGAGCTCTCC TTCCAGCCCT CCCAGCCCCT CAGCAAGTCC
    AGCTCCTCCC CTGAGCTGCA GACCCTGCAG GACATACTCG GGGACCCCGG GGACAAGTCT
    GATGTTGGCC GGCTCAGCCC TGAGGCCAAG GCCCGGTCAC AGTCCGGGAT TCTGGACGGG
    GTGGGTGCCT CCTGGTCAGC CCCAGGTGAA GAGAACCAGG GCCAGGGCCC CGCACAGCCC
    GAGGGTCCCT TGCCTTCCAG CTGCCCCCGC TCCCCCAGTG GCCTGCGGCC CAGAGGCTAC
    ACCATCTCTG ATTCAGCCCC GTCACGCAGA GGCAAGAGGG TAGAGCGGGA CGCCTTCAGG
    AGCAGAGCTG GGGCTTCCAA CACTGAGAAG GTGCCAGGCA TCAATCCCAG CTTTGTGTTC
    CTGCAGCTCT ACCACTCACC CTTCTTTGGT GACGAGTCCA ACAAGCCCAT CCTTCTACCC
    AATGAGTCCT TCGAGCGGTC AGTGCAGCTT CTCGACCAGA TTCCATCATA CGACACACAC
    AAGATTGCCG TCCTGTACGT GGGAGAAGGC CAGAGCAGCA GTGAGCTCGC CATCCTGTCC
    AATGAGCATG GCTCGTACAG GTACACGGAG TTCCTGACAG GCCTGGGCAA GCTCATTGAG
    CTCAAAGACT GCCAGCCGGA CAAGGTGTAC CTGGGGGGCC TGGACGTGTG TGGCGAAGAT
    GGCCAGTTCA CCTACTGCTG GCATGACGAC ATCATGCAAG CCGTCTTCCA CATTGCTACC
    CTGATGCCCA CCAAGGAAGT GGACAAGCAC CGCTGTGACA AGAAGCGCCA CCTGGGCAAT
    GACTTTGTGT CCATCGTCTA CAACGACTCT GGTGAGGACT TCAAGCTGGG CACCATCCGA
    GGCCAGTTCA ACTTCGTCCA CGTGATCATC ACCCCCCTGG ACTATGAGTG CAACCTGGTG
    TCCCTGCAGT GCAGGAAAGA CATGGAAGGT CTTGTGGATA CTAGCGTGGC CAAGATCGTG
    TCTGACCGCA ACTTGCCTTT TGTGGCACGT CAGATGGCTT TGCATGCAAA TATGGCCTCA
    CAAGTGCATC ATAGCCGCTC CAACCCCACC GACATCTATC CCTCCAAGTG GATTGCCAGG
    CTTCGCCACA TCAAGCGACT CCGCCACCGG ATCCGCGAGG AAGCCCACTA CACCAACCCC
    AGCCTGCCAC TGATGCAGAT GCACCCCCCG GGCCACCCCA AAGCCTCTGC TCAGGCGCCG
    GCAGAGCCTG TGCCCACGTA TGAGACAGGC CAGCGGAAGC GTCTCATCTC CTCTGTGGAT
    GACTTCACGG AGTTTGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe08328
    Clone ID Related Accession (Same CDS sequence) XM_005621715.2 , XM_005621715.3
    Accession Version XM_005621715.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5442bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product tuberin isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005621715.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
    CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC
    ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG
    ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG
    CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA
    GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG
    GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG
    GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG
    GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT
    GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC
    ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC
    GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG
    CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG
    GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG
    ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG
    CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC
    TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC
    CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC
    AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC
    CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG
    TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC
    TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT
    GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA
    GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT
    CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG
    AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC
    AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT
    AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG
    CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC
    AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT
    GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG
    CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG
    GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG
    GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG
    CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC
    GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG
    GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC
    GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC
    ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC
    GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG
    TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC
    CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC
    CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC
    AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT
    CCACCCGTGA AAGAATTCAA AGAGAGCTCT GCCGCCGAGG CCTTCCGATG CCGCAGCATC
    AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATCCAGACGT CCCTCACAAG CGCCAGCTTG
    GGGTCTGCTG ACGAGAACTC GATGGCCCAG GCTGATGACA ACTTGAAAAA TCTCCACCTG
    GAGCTCACGG AAACATGTCT GGACATGATG GCCAGATATG TGTTCTCCAA CTTCACAGCA
    GTCCCCAAGA GGTCCCCGGT GGGAGAGTTC CTCCTGGCTG GTGGCAGGAC CAAAACCTGG
    CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACGAGCGTGG GGACGGGGAC CCGGTCGCTG
    CTGGGCTTGG ACTCAGGAGA TCAGCAAGGT GGCCCCGAGT TGAGCTCGGA CCCTGGCATG
    CATGTGAGAC AGGCAAAGGA GGCACCTGCC AAGCTGGAGT CGCAGGCTGG GCAGCAGGTG
    TGCCGCGGCG CCCGGGACCG GGTCCGCTCC ATGTCCGGGG GCCACGGTCT TCGTGTTGGC
    GCCCTGGACA CTCCAGCCTC CCACTTGCCG GGTGGCCCCA CTTCCCCAGG CACGCAGGCA
    GCGCTGGCCA GCAAGTCTGA CAGGGCCTCG GCTGGCACCC GGTTCCCAGC ACAGGAGAAG
    ACGAGCCTGG CAGCCTACGT ACCGCTGCTG ACCCAGGGCT GGGCGGAGAT CTTGGTCCGG
    AGGCCTACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCCTTCTCG
    TCGGACATCA ACAACATGCC TCTGCAGGAG CTGTCCAACG CGCTCATGGC CGCTGAGCGC
    TTCAAGGAGC GTCGTGACAC GGCTCTGTAC AAGTCGCTGT CGGTGCCTGC CGCAGGCTCG
    GCCAAGCCCC CTCCGCCCCC GAGATCTAAC ACGGTGGCCT CTTTCTCCTC CCTGTACCAG
    TCCAGTTGCC AAGGAAAGCT GCACAGGAGC ATTTCCTGGG CAGACTCTGC GGTGGTTCTG
    GAGGAGGGAA ATTCAAGCGA GACCAGTCTG CCAGTGGAGC ACACGGAGCT GGAGGACTTC
    GAGGCGACAC TAGGCACAGA CCGGCGCTGT GGCCGCGCGG AAGCTTACAG CAGGTCGTCT
    TCAAGCTCCA GCCAGGAGGA GAAGTTCCCT GCAGAGGAGC TGGCTGCCGG AGGGATCCCC
    ATCGAACGAG CTGTCTCTTC CGAGGGTGCC CGGCCTTCTG TGGAGCTCTC CTTCCAGCCC
    TCCCAGCCCC TCAGCAAGTC CAGCTCCTCC CCTGAGCTGC AGACCCTGCA GGACATACTC
    GGGGACCCCG GGGACAAGTC TGATGTTGGC CGGCTCAGCC CTGAGGCCAA GGCCCGGTCA
    CAGTCCGGGA TTCTGGACGG GGTGGGTGCC TCCTGGTCAG CCCCAGGTGA AGAGAACCAG
    GGCCAGGGCC CCGCACAGCC CGAGGGTCCC TTGCCTTCCA GCTGCCCCCG CTCCCCCAGT
    GGCCTGCGGC CCAGAGGCTA CACCATCTCT GATTCAGCCC CGTCACGCAG AGGCAAGAGG
    GTAGAGCGGG ACGCCTTCAG GAGCAGAGCT GGGGCTTCCA ACACTGAGAA GGTGCCAGGC
    ATCAATCCCA GCTTTGTGTT CCTGCAGCTC TACCACTCAC CCTTCTTTGG TGACGAGTCC
    AACAAGCCCA TCCTTCTACC CAATGAGTCC TTCGAGCGGT CAGTGCAGCT TCTCGACCAG
    ATTCCATCAT ACGACACACA CAAGATTGCC GTCCTGTACG TGGGAGAAGG CCAGAGCAGC
    AGTGAGCTCG CCATCCTGTC CAATGAGCAT GGCTCGTACA GGTACACGGA GTTCCTGACA
    GGCCTGGGCA AGCTCATTGA GCTCAAAGAC TGCCAGCCGG ACAAGGTGTA CCTGGGGGGC
    CTGGACGTGT GTGGCGAAGA TGGCCAGTTC ACCTACTGCT GGCATGACGA CATCATGCAA
    GCCGTCTTCC ACATTGCTAC CCTGATGCCC ACCAAGGAAG TGGACAAGCA CCGCTGTGAC
    AAGAAGCGCC ACCTGGGCAA TGACTTTGTG TCCATCGTCT ACAACGACTC TGGTGAGGAC
    TTCAAGCTGG GCACCATCCG AGGCCAGTTC AACTTCGTCC ACGTGATCAT CACCCCCCTG
    GACTATGAGT GCAACCTGGT GTCCCTGCAG TGCAGGAAAG ACATGGAAGG TCTTGTGGAT
    ACTAGCGTGG CCAAGATCGT GTCTGACCGC AACTTGCCTT TTGTGGCACG TCAGATGGCT
    TTGCATGCAA ATATGGCCTC ACAAGTGCAT CATAGCCGCT CCAACCCCAC CGACATCTAT
    CCCTCCAAGT GGATTGCCAG GCTTCGCCAC ATCAAGCGAC TCCGCCACCG GATCCGCGAG
    GAAGCCCACT ACACCAACCC CAGCCTGCCA CTGATGCAGA TGCACCCCCC GGGCCACCCC
    AAAGCCTCTG CTCAGGCGCC GGCAGAGCCT GTGCCCACGT ATGAGACAGG CCAGCGGAAG
    CGTCTCATCT CCTCTGTGGA TGACTTCACG GAGTTTGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005621772.1
    CDS283..5724
    Translation

    Target ORF information:

    RefSeq Version XM_005621715.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris tuberous sclerosis 2 (TSC2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005621715.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
    CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC
    ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG
    ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG
    CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA
    GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG
    GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG
    GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG
    GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT
    GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC
    ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC
    GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG
    CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG
    GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG
    ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG
    CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC
    TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC
    CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC
    AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC
    CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG
    TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC
    TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT
    GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA
    GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT
    CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG
    AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC
    AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT
    AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG
    CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC
    AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT
    GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG
    CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG
    GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG
    GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG
    CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC
    GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG
    GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC
    GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC
    ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC
    GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG
    TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC
    CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC
    CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC
    AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT
    CCACCCGTGA AAGAATTCAA AGAGAGCTCT GCCGCCGAGG CCTTCCGATG CCGCAGCATC
    AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATCCAGACGT CCCTCACAAG CGCCAGCTTG
    GGGTCTGCTG ACGAGAACTC GATGGCCCAG GCTGATGACA ACTTGAAAAA TCTCCACCTG
    GAGCTCACGG AAACATGTCT GGACATGATG GCCAGATATG TGTTCTCCAA CTTCACAGCA
    GTCCCCAAGA GGTCCCCGGT GGGAGAGTTC CTCCTGGCTG GTGGCAGGAC CAAAACCTGG
    CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACGAGCGTGG GGACGGGGAC CCGGTCGCTG
    CTGGGCTTGG ACTCAGGAGA TCAGCAAGGT GGCCCCGAGT TGAGCTCGGA CCCTGGCATG
    CATGTGAGAC AGGCAAAGGA GGCACCTGCC AAGCTGGAGT CGCAGGCTGG GCAGCAGGTG
    TGCCGCGGCG CCCGGGACCG GGTCCGCTCC ATGTCCGGGG GCCACGGTCT TCGTGTTGGC
    GCCCTGGACA CTCCAGCCTC CCACTTGCCG GGTGGCCCCA CTTCCCCAGG CACGCAGGCA
    GCGCTGGCCA GCAAGTCTGA CAGGGCCTCG GCTGGCACCC GGTTCCCAGC ACAGGAGAAG
    ACGAGCCTGG CAGCCTACGT ACCGCTGCTG ACCCAGGGCT GGGCGGAGAT CTTGGTCCGG
    AGGCCTACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCCTTCTCG
    TCGGACATCA ACAACATGCC TCTGCAGGAG CTGTCCAACG CGCTCATGGC CGCTGAGCGC
    TTCAAGGAGC GTCGTGACAC GGCTCTGTAC AAGTCGCTGT CGGTGCCTGC CGCAGGCTCG
    GCCAAGCCCC CTCCGCCCCC GAGATCTAAC ACGGTGGCCT CTTTCTCCTC CCTGTACCAG
    TCCAGTTGCC AAGGAAAGCT GCACAGGAGC ATTTCCTGGG CAGACTCTGC GGTGGTTCTG
    GAGGAGGGAA ATTCAAGCGA GACCAGTCTG CCAGTGGAGC ACACGGAGCT GGAGGACTTC
    GAGGCGACAC TAGGCACAGA CCGGCGCTGT GGCCGCGCGG AAGCTTACAG CAGGTCGTCT
    TCAAGCTCCA GCCAGGAGGA GAAGTTCCCT GCAGAGGAGC TGGCTGCCGG AGGGATCCCC
    ATCGAACGAG CTGTCTCTTC CGAGGGTGCC CGGCCTTCTG TGGAGCTCTC CTTCCAGCCC
    TCCCAGCCCC TCAGCAAGTC CAGCTCCTCC CCTGAGCTGC AGACCCTGCA GGACATACTC
    GGGGACCCCG GGGACAAGTC TGATGTTGGC CGGCTCAGCC CTGAGGCCAA GGCCCGGTCA
    CAGTCCGGGA TTCTGGACGG GGTGGGTGCC TCCTGGTCAG CCCCAGGTGA AGAGAACCAG
    GGCCAGGGCC CCGCACAGCC CGAGGGTCCC TTGCCTTCCA GCTGCCCCCG CTCCCCCAGT
    GGCCTGCGGC CCAGAGGCTA CACCATCTCT GATTCAGCCC CGTCACGCAG AGGCAAGAGG
    GTAGAGCGGG ACGCCTTCAG GAGCAGAGCT GGGGCTTCCA ACACTGAGAA GGTGCCAGGC
    ATCAATCCCA GCTTTGTGTT CCTGCAGCTC TACCACTCAC CCTTCTTTGG TGACGAGTCC
    AACAAGCCCA TCCTTCTACC CAATGAGTCC TTCGAGCGGT CAGTGCAGCT TCTCGACCAG
    ATTCCATCAT ACGACACACA CAAGATTGCC GTCCTGTACG TGGGAGAAGG CCAGAGCAGC
    AGTGAGCTCG CCATCCTGTC CAATGAGCAT GGCTCGTACA GGTACACGGA GTTCCTGACA
    GGCCTGGGCA AGCTCATTGA GCTCAAAGAC TGCCAGCCGG ACAAGGTGTA CCTGGGGGGC
    CTGGACGTGT GTGGCGAAGA TGGCCAGTTC ACCTACTGCT GGCATGACGA CATCATGCAA
    GCCGTCTTCC ACATTGCTAC CCTGATGCCC ACCAAGGAAG TGGACAAGCA CCGCTGTGAC
    AAGAAGCGCC ACCTGGGCAA TGACTTTGTG TCCATCGTCT ACAACGACTC TGGTGAGGAC
    TTCAAGCTGG GCACCATCCG AGGCCAGTTC AACTTCGTCC ACGTGATCAT CACCCCCCTG
    GACTATGAGT GCAACCTGGT GTCCCTGCAG TGCAGGAAAG ACATGGAAGG TCTTGTGGAT
    ACTAGCGTGG CCAAGATCGT GTCTGACCGC AACTTGCCTT TTGTGGCACG TCAGATGGCT
    TTGCATGCAA ATATGGCCTC ACAAGTGCAT CATAGCCGCT CCAACCCCAC CGACATCTAT
    CCCTCCAAGT GGATTGCCAG GCTTCGCCAC ATCAAGCGAC TCCGCCACCG GATCCGCGAG
    GAAGCCCACT ACACCAACCC CAGCCTGCCA CTGATGCAGA TGCACCCCCC GGGCCACCCC
    AAAGCCTCTG CTCAGGCGCC GGCAGAGCCT GTGCCCACGT ATGAGACAGG CCAGCGGAAG
    CGTCTCATCT CCTCTGTGGA TGACTTCACG GAGTTTGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe08328
    Clone ID Related Accession (Same CDS sequence) XM_005621715.2 , XM_005621715.3
    Accession Version XM_005621715.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5442bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product tuberin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005621715.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
    CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC
    ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG
    ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG
    CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA
    GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG
    GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG
    GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG
    GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT
    GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC
    ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC
    GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG
    CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG
    GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG
    ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG
    CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC
    TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC
    CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC
    AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC
    CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG
    TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC
    TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT
    GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA
    GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT
    CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG
    AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC
    AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT
    AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG
    CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC
    AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT
    GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG
    CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG
    GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG
    GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG
    CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC
    GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG
    GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC
    GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC
    ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC
    GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG
    TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC
    CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC
    CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC
    AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT
    CCACCCGTGA AAGAATTCAA AGAGAGCTCT GCCGCCGAGG CCTTCCGATG CCGCAGCATC
    AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATCCAGACGT CCCTCACAAG CGCCAGCTTG
    GGGTCTGCTG ACGAGAACTC GATGGCCCAG GCTGATGACA ACTTGAAAAA TCTCCACCTG
    GAGCTCACGG AAACATGTCT GGACATGATG GCCAGATATG TGTTCTCCAA CTTCACAGCA
    GTCCCCAAGA GGTCCCCGGT GGGAGAGTTC CTCCTGGCTG GTGGCAGGAC CAAAACCTGG
    CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACGAGCGTGG GGACGGGGAC CCGGTCGCTG
    CTGGGCTTGG ACTCAGGAGA TCAGCAAGGT GGCCCCGAGT TGAGCTCGGA CCCTGGCATG
    CATGTGAGAC AGGCAAAGGA GGCACCTGCC AAGCTGGAGT CGCAGGCTGG GCAGCAGGTG
    TGCCGCGGCG CCCGGGACCG GGTCCGCTCC ATGTCCGGGG GCCACGGTCT TCGTGTTGGC
    GCCCTGGACA CTCCAGCCTC CCACTTGCCG GGTGGCCCCA CTTCCCCAGG CACGCAGGCA
    GCGCTGGCCA GCAAGTCTGA CAGGGCCTCG GCTGGCACCC GGTTCCCAGC ACAGGAGAAG
    ACGAGCCTGG CAGCCTACGT ACCGCTGCTG ACCCAGGGCT GGGCGGAGAT CTTGGTCCGG
    AGGCCTACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCCTTCTCG
    TCGGACATCA ACAACATGCC TCTGCAGGAG CTGTCCAACG CGCTCATGGC CGCTGAGCGC
    TTCAAGGAGC GTCGTGACAC GGCTCTGTAC AAGTCGCTGT CGGTGCCTGC CGCAGGCTCG
    GCCAAGCCCC CTCCGCCCCC GAGATCTAAC ACGGTGGCCT CTTTCTCCTC CCTGTACCAG
    TCCAGTTGCC AAGGAAAGCT GCACAGGAGC ATTTCCTGGG CAGACTCTGC GGTGGTTCTG
    GAGGAGGGAA ATTCAAGCGA GACCAGTCTG CCAGTGGAGC ACACGGAGCT GGAGGACTTC
    GAGGCGACAC TAGGCACAGA CCGGCGCTGT GGCCGCGCGG AAGCTTACAG CAGGTCGTCT
    TCAAGCTCCA GCCAGGAGGA GAAGTTCCCT GCAGAGGAGC TGGCTGCCGG AGGGATCCCC
    ATCGAACGAG CTGTCTCTTC CGAGGGTGCC CGGCCTTCTG TGGAGCTCTC CTTCCAGCCC
    TCCCAGCCCC TCAGCAAGTC CAGCTCCTCC CCTGAGCTGC AGACCCTGCA GGACATACTC
    GGGGACCCCG GGGACAAGTC TGATGTTGGC CGGCTCAGCC CTGAGGCCAA GGCCCGGTCA
    CAGTCCGGGA TTCTGGACGG GGTGGGTGCC TCCTGGTCAG CCCCAGGTGA AGAGAACCAG
    GGCCAGGGCC CCGCACAGCC CGAGGGTCCC TTGCCTTCCA GCTGCCCCCG CTCCCCCAGT
    GGCCTGCGGC CCAGAGGCTA CACCATCTCT GATTCAGCCC CGTCACGCAG AGGCAAGAGG
    GTAGAGCGGG ACGCCTTCAG GAGCAGAGCT GGGGCTTCCA ACACTGAGAA GGTGCCAGGC
    ATCAATCCCA GCTTTGTGTT CCTGCAGCTC TACCACTCAC CCTTCTTTGG TGACGAGTCC
    AACAAGCCCA TCCTTCTACC CAATGAGTCC TTCGAGCGGT CAGTGCAGCT TCTCGACCAG
    ATTCCATCAT ACGACACACA CAAGATTGCC GTCCTGTACG TGGGAGAAGG CCAGAGCAGC
    AGTGAGCTCG CCATCCTGTC CAATGAGCAT GGCTCGTACA GGTACACGGA GTTCCTGACA
    GGCCTGGGCA AGCTCATTGA GCTCAAAGAC TGCCAGCCGG ACAAGGTGTA CCTGGGGGGC
    CTGGACGTGT GTGGCGAAGA TGGCCAGTTC ACCTACTGCT GGCATGACGA CATCATGCAA
    GCCGTCTTCC ACATTGCTAC CCTGATGCCC ACCAAGGAAG TGGACAAGCA CCGCTGTGAC
    AAGAAGCGCC ACCTGGGCAA TGACTTTGTG TCCATCGTCT ACAACGACTC TGGTGAGGAC
    TTCAAGCTGG GCACCATCCG AGGCCAGTTC AACTTCGTCC ACGTGATCAT CACCCCCCTG
    GACTATGAGT GCAACCTGGT GTCCCTGCAG TGCAGGAAAG ACATGGAAGG TCTTGTGGAT
    ACTAGCGTGG CCAAGATCGT GTCTGACCGC AACTTGCCTT TTGTGGCACG TCAGATGGCT
    TTGCATGCAA ATATGGCCTC ACAAGTGCAT CATAGCCGCT CCAACCCCAC CGACATCTAT
    CCCTCCAAGT GGATTGCCAG GCTTCGCCAC ATCAAGCGAC TCCGCCACCG GATCCGCGAG
    GAAGCCCACT ACACCAACCC CAGCCTGCCA CTGATGCAGA TGCACCCCCC GGGCCACCCC
    AAAGCCTCTG CTCAGGCGCC GGCAGAGCCT GTGCCCACGT ATGAGACAGG CCAGCGGAAG
    CGTCTCATCT CCTCTGTGGA TGACTTCACG GAGTTTGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005621772.1
    CDS290..5731
    Translation

    Target ORF information:

    RefSeq Version XM_005621715.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005621715.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
    CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC
    ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG
    ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG
    CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA
    GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG
    GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG
    GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG
    GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT
    GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC
    ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC
    GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG
    CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG
    GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG
    ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG
    CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC
    TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC
    CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC
    AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC
    CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG
    TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC
    TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT
    GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA
    GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT
    CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG
    AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC
    AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT
    AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG
    CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC
    AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT
    GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG
    CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG
    GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG
    GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG
    CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC
    GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG
    GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC
    GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC
    ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC
    GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG
    TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC
    CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC
    CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC
    AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT
    CCACCCGTGA AAGAATTCAA AGAGAGCTCT GCCGCCGAGG CCTTCCGATG CCGCAGCATC
    AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATCCAGACGT CCCTCACAAG CGCCAGCTTG
    GGGTCTGCTG ACGAGAACTC GATGGCCCAG GCTGATGACA ACTTGAAAAA TCTCCACCTG
    GAGCTCACGG AAACATGTCT GGACATGATG GCCAGATATG TGTTCTCCAA CTTCACAGCA
    GTCCCCAAGA GGTCCCCGGT GGGAGAGTTC CTCCTGGCTG GTGGCAGGAC CAAAACCTGG
    CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACGAGCGTGG GGACGGGGAC CCGGTCGCTG
    CTGGGCTTGG ACTCAGGAGA TCAGCAAGGT GGCCCCGAGT TGAGCTCGGA CCCTGGCATG
    CATGTGAGAC AGGCAAAGGA GGCACCTGCC AAGCTGGAGT CGCAGGCTGG GCAGCAGGTG
    TGCCGCGGCG CCCGGGACCG GGTCCGCTCC ATGTCCGGGG GCCACGGTCT TCGTGTTGGC
    GCCCTGGACA CTCCAGCCTC CCACTTGCCG GGTGGCCCCA CTTCCCCAGG CACGCAGGCA
    GCGCTGGCCA GCAAGTCTGA CAGGGCCTCG GCTGGCACCC GGTTCCCAGC ACAGGAGAAG
    ACGAGCCTGG CAGCCTACGT ACCGCTGCTG ACCCAGGGCT GGGCGGAGAT CTTGGTCCGG
    AGGCCTACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCCTTCTCG
    TCGGACATCA ACAACATGCC TCTGCAGGAG CTGTCCAACG CGCTCATGGC CGCTGAGCGC
    TTCAAGGAGC GTCGTGACAC GGCTCTGTAC AAGTCGCTGT CGGTGCCTGC CGCAGGCTCG
    GCCAAGCCCC CTCCGCCCCC GAGATCTAAC ACGGTGGCCT CTTTCTCCTC CCTGTACCAG
    TCCAGTTGCC AAGGAAAGCT GCACAGGAGC ATTTCCTGGG CAGACTCTGC GGTGGTTCTG
    GAGGAGGGAA ATTCAAGCGA GACCAGTCTG CCAGTGGAGC ACACGGAGCT GGAGGACTTC
    GAGGCGACAC TAGGCACAGA CCGGCGCTGT GGCCGCGCGG AAGCTTACAG CAGGTCGTCT
    TCAAGCTCCA GCCAGGAGGA GAAGTTCCCT GCAGAGGAGC TGGCTGCCGG AGGGATCCCC
    ATCGAACGAG CTGTCTCTTC CGAGGGTGCC CGGCCTTCTG TGGAGCTCTC CTTCCAGCCC
    TCCCAGCCCC TCAGCAAGTC CAGCTCCTCC CCTGAGCTGC AGACCCTGCA GGACATACTC
    GGGGACCCCG GGGACAAGTC TGATGTTGGC CGGCTCAGCC CTGAGGCCAA GGCCCGGTCA
    CAGTCCGGGA TTCTGGACGG GGTGGGTGCC TCCTGGTCAG CCCCAGGTGA AGAGAACCAG
    GGCCAGGGCC CCGCACAGCC CGAGGGTCCC TTGCCTTCCA GCTGCCCCCG CTCCCCCAGT
    GGCCTGCGGC CCAGAGGCTA CACCATCTCT GATTCAGCCC CGTCACGCAG AGGCAAGAGG
    GTAGAGCGGG ACGCCTTCAG GAGCAGAGCT GGGGCTTCCA ACACTGAGAA GGTGCCAGGC
    ATCAATCCCA GCTTTGTGTT CCTGCAGCTC TACCACTCAC CCTTCTTTGG TGACGAGTCC
    AACAAGCCCA TCCTTCTACC CAATGAGTCC TTCGAGCGGT CAGTGCAGCT TCTCGACCAG
    ATTCCATCAT ACGACACACA CAAGATTGCC GTCCTGTACG TGGGAGAAGG CCAGAGCAGC
    AGTGAGCTCG CCATCCTGTC CAATGAGCAT GGCTCGTACA GGTACACGGA GTTCCTGACA
    GGCCTGGGCA AGCTCATTGA GCTCAAAGAC TGCCAGCCGG ACAAGGTGTA CCTGGGGGGC
    CTGGACGTGT GTGGCGAAGA TGGCCAGTTC ACCTACTGCT GGCATGACGA CATCATGCAA
    GCCGTCTTCC ACATTGCTAC CCTGATGCCC ACCAAGGAAG TGGACAAGCA CCGCTGTGAC
    AAGAAGCGCC ACCTGGGCAA TGACTTTGTG TCCATCGTCT ACAACGACTC TGGTGAGGAC
    TTCAAGCTGG GCACCATCCG AGGCCAGTTC AACTTCGTCC ACGTGATCAT CACCCCCCTG
    GACTATGAGT GCAACCTGGT GTCCCTGCAG TGCAGGAAAG ACATGGAAGG TCTTGTGGAT
    ACTAGCGTGG CCAAGATCGT GTCTGACCGC AACTTGCCTT TTGTGGCACG TCAGATGGCT
    TTGCATGCAA ATATGGCCTC ACAAGTGCAT CATAGCCGCT CCAACCCCAC CGACATCTAT
    CCCTCCAAGT GGATTGCCAG GCTTCGCCAC ATCAAGCGAC TCCGCCACCG GATCCGCGAG
    GAAGCCCACT ACACCAACCC CAGCCTGCCA CTGATGCAGA TGCACCCCCC GGGCCACCCC
    AAAGCCTCTG CTCAGGCGCC GGCAGAGCCT GTGCCCACGT ATGAGACAGG CCAGCGGAAG
    CGTCTCATCT CCTCTGTGGA TGACTTCACG GAGTTTGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe08329
    Clone ID Related Accession (Same CDS sequence) XM_005621716.2 , XM_005621716.3
    Accession Version XM_005621716.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5439bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product tuberin isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005621716.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
    CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC
    ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG
    ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG
    CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA
    GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG
    GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG
    GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG
    GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT
    GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC
    ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC
    GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG
    CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG
    GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG
    ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG
    CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC
    TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC
    CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC
    AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC
    CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG
    TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC
    TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT
    GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA
    GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT
    CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG
    AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC
    AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT
    AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG
    CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC
    AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT
    GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG
    CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG
    GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG
    GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG
    CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC
    GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG
    GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC
    GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC
    ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC
    GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG
    TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC
    CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC
    CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC
    AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT
    CCACCCGTGA AAGAATTCAA AGAGAGCTCT GCCGCCGAGG CCTTCCGATG CCGCAGCATC
    AGTGTGTCTG AACATGTGGT CCGCAGGATC CAGACGTCCC TCACAAGCGC CAGCTTGGGG
    TCTGCTGACG AGAACTCGAT GGCCCAGGCT GATGACAACT TGAAAAATCT CCACCTGGAG
    CTCACGGAAA CATGTCTGGA CATGATGGCC AGATATGTGT TCTCCAACTT CACAGCAGTC
    CCCAAGAGGT CCCCGGTGGG AGAGTTCCTC CTGGCTGGTG GCAGGACCAA AACCTGGCTG
    GTTGGGAACA AGCTTGTCAC TGTGACGACG AGCGTGGGGA CGGGGACCCG GTCGCTGCTG
    GGCTTGGACT CAGGAGATCA GCAAGGTGGC CCCGAGTTGA GCTCGGACCC TGGCATGCAT
    GTGAGACAGG CAAAGGAGGC ACCTGCCAAG CTGGAGTCGC AGGCTGGGCA GCAGGTGTGC
    CGCGGCGCCC GGGACCGGGT CCGCTCCATG TCCGGGGGCC ACGGTCTTCG TGTTGGCGCC
    CTGGACACTC CAGCCTCCCA CTTGCCGGGT GGCCCCACTT CCCCAGGCAC GCAGGCAGCG
    CTGGCCAGCA AGTCTGACAG GGCCTCGGCT GGCACCCGGT TCCCAGCACA GGAGAAGACG
    AGCCTGGCAG CCTACGTACC GCTGCTGACC CAGGGCTGGG CGGAGATCTT GGTCCGGAGG
    CCTACAGGGA ACACCAGCTG GCTGATGAGC CTGGAGAACC CGCTCAGCCC CTTCTCGTCG
    GACATCAACA ACATGCCTCT GCAGGAGCTG TCCAACGCGC TCATGGCCGC TGAGCGCTTC
    AAGGAGCGTC GTGACACGGC TCTGTACAAG TCGCTGTCGG TGCCTGCCGC AGGCTCGGCC
    AAGCCCCCTC CGCCCCCGAG ATCTAACACG GTGGCCTCTT TCTCCTCCCT GTACCAGTCC
    AGTTGCCAAG GAAAGCTGCA CAGGAGCATT TCCTGGGCAG ACTCTGCGGT GGTTCTGGAG
    GAGGGAAATT CAAGCGAGAC CAGTCTGCCA GTGGAGCACA CGGAGCTGGA GGACTTCGAG
    GCGACACTAG GCACAGACCG GCGCTGTGGC CGCGCGGAAG CTTACAGCAG GTCGTCTTCA
    AGCTCCAGCC AGGAGGAGAA GTTCCCTGCA GAGGAGCTGG CTGCCGGAGG GATCCCCATC
    GAACGAGCTG TCTCTTCCGA GGGTGCCCGG CCTTCTGTGG AGCTCTCCTT CCAGCCCTCC
    CAGCCCCTCA GCAAGTCCAG CTCCTCCCCT GAGCTGCAGA CCCTGCAGGA CATACTCGGG
    GACCCCGGGG ACAAGTCTGA TGTTGGCCGG CTCAGCCCTG AGGCCAAGGC CCGGTCACAG
    TCCGGGATTC TGGACGGGGT GGGTGCCTCC TGGTCAGCCC CAGGTGAAGA GAACCAGGGC
    CAGGGCCCCG CACAGCCCGA GGGTCCCTTG CCTTCCAGCT GCCCCCGCTC CCCCAGTGGC
    CTGCGGCCCA GAGGCTACAC CATCTCTGAT TCAGCCCCGT CACGCAGAGG CAAGAGGGTA
    GAGCGGGACG CCTTCAGGAG CAGAGCTGGG GCTTCCAACA CTGAGAAGGT GCCAGGCATC
    AATCCCAGCT TTGTGTTCCT GCAGCTCTAC CACTCACCCT TCTTTGGTGA CGAGTCCAAC
    AAGCCCATCC TTCTACCCAA TGAGTCCTTC GAGCGGTCAG TGCAGCTTCT CGACCAGATT
    CCATCATACG ACACACACAA GATTGCCGTC CTGTACGTGG GAGAAGGCCA GAGCAGCAGT
    GAGCTCGCCA TCCTGTCCAA TGAGCATGGC TCGTACAGGT ACACGGAGTT CCTGACAGGC
    CTGGGCAAGC TCATTGAGCT CAAAGACTGC CAGCCGGACA AGGTGTACCT GGGGGGCCTG
    GACGTGTGTG GCGAAGATGG CCAGTTCACC TACTGCTGGC ATGACGACAT CATGCAAGCC
    GTCTTCCACA TTGCTACCCT GATGCCCACC AAGGAAGTGG ACAAGCACCG CTGTGACAAG
    AAGCGCCACC TGGGCAATGA CTTTGTGTCC ATCGTCTACA ACGACTCTGG TGAGGACTTC
    AAGCTGGGCA CCATCCGAGG CCAGTTCAAC TTCGTCCACG TGATCATCAC CCCCCTGGAC
    TATGAGTGCA ACCTGGTGTC CCTGCAGTGC AGGAAAGACA TGGAAGGTCT TGTGGATACT
    AGCGTGGCCA AGATCGTGTC TGACCGCAAC TTGCCTTTTG TGGCACGTCA GATGGCTTTG
    CATGCAAATA TGGCCTCACA AGTGCATCAT AGCCGCTCCA ACCCCACCGA CATCTATCCC
    TCCAAGTGGA TTGCCAGGCT TCGCCACATC AAGCGACTCC GCCACCGGAT CCGCGAGGAA
    GCCCACTACA CCAACCCCAG CCTGCCACTG ATGCAGATGC ACCCCCCGGG CCACCCCAAA
    GCCTCTGCTC AGGCGCCGGC AGAGCCTGTG CCCACGTATG AGACAGGCCA GCGGAAGCGT
    CTCATCTCCT CTGTGGATGA CTTCACGGAG TTTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005621773.1
    CDS283..5721
    Translation

    Target ORF information:

    RefSeq Version XM_005621716.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris tuberous sclerosis 2 (TSC2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005621716.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
    CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC
    ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG
    ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG
    CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA
    GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG
    GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG
    GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG
    GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT
    GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC
    ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC
    GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG
    CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG
    GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG
    ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG
    CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC
    TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC
    CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC
    AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC
    CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG
    TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC
    TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT
    GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA
    GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT
    CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG
    AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC
    AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT
    AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG
    CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC
    AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT
    GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG
    CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG
    GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG
    GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG
    CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC
    GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG
    GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC
    GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC
    ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC
    GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG
    TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC
    CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC
    CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC
    AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT
    CCACCCGTGA AAGAATTCAA AGAGAGCTCT GCCGCCGAGG CCTTCCGATG CCGCAGCATC
    AGTGTGTCTG AACATGTGGT CCGCAGGATC CAGACGTCCC TCACAAGCGC CAGCTTGGGG
    TCTGCTGACG AGAACTCGAT GGCCCAGGCT GATGACAACT TGAAAAATCT CCACCTGGAG
    CTCACGGAAA CATGTCTGGA CATGATGGCC AGATATGTGT TCTCCAACTT CACAGCAGTC
    CCCAAGAGGT CCCCGGTGGG AGAGTTCCTC CTGGCTGGTG GCAGGACCAA AACCTGGCTG
    GTTGGGAACA AGCTTGTCAC TGTGACGACG AGCGTGGGGA CGGGGACCCG GTCGCTGCTG
    GGCTTGGACT CAGGAGATCA GCAAGGTGGC CCCGAGTTGA GCTCGGACCC TGGCATGCAT
    GTGAGACAGG CAAAGGAGGC ACCTGCCAAG CTGGAGTCGC AGGCTGGGCA GCAGGTGTGC
    CGCGGCGCCC GGGACCGGGT CCGCTCCATG TCCGGGGGCC ACGGTCTTCG TGTTGGCGCC
    CTGGACACTC CAGCCTCCCA CTTGCCGGGT GGCCCCACTT CCCCAGGCAC GCAGGCAGCG
    CTGGCCAGCA AGTCTGACAG GGCCTCGGCT GGCACCCGGT TCCCAGCACA GGAGAAGACG
    AGCCTGGCAG CCTACGTACC GCTGCTGACC CAGGGCTGGG CGGAGATCTT GGTCCGGAGG
    CCTACAGGGA ACACCAGCTG GCTGATGAGC CTGGAGAACC CGCTCAGCCC CTTCTCGTCG
    GACATCAACA ACATGCCTCT GCAGGAGCTG TCCAACGCGC TCATGGCCGC TGAGCGCTTC
    AAGGAGCGTC GTGACACGGC TCTGTACAAG TCGCTGTCGG TGCCTGCCGC AGGCTCGGCC
    AAGCCCCCTC CGCCCCCGAG ATCTAACACG GTGGCCTCTT TCTCCTCCCT GTACCAGTCC
    AGTTGCCAAG GAAAGCTGCA CAGGAGCATT TCCTGGGCAG ACTCTGCGGT GGTTCTGGAG
    GAGGGAAATT CAAGCGAGAC CAGTCTGCCA GTGGAGCACA CGGAGCTGGA GGACTTCGAG
    GCGACACTAG GCACAGACCG GCGCTGTGGC CGCGCGGAAG CTTACAGCAG GTCGTCTTCA
    AGCTCCAGCC AGGAGGAGAA GTTCCCTGCA GAGGAGCTGG CTGCCGGAGG GATCCCCATC
    GAACGAGCTG TCTCTTCCGA GGGTGCCCGG CCTTCTGTGG AGCTCTCCTT CCAGCCCTCC
    CAGCCCCTCA GCAAGTCCAG CTCCTCCCCT GAGCTGCAGA CCCTGCAGGA CATACTCGGG
    GACCCCGGGG ACAAGTCTGA TGTTGGCCGG CTCAGCCCTG AGGCCAAGGC CCGGTCACAG
    TCCGGGATTC TGGACGGGGT GGGTGCCTCC TGGTCAGCCC CAGGTGAAGA GAACCAGGGC
    CAGGGCCCCG CACAGCCCGA GGGTCCCTTG CCTTCCAGCT GCCCCCGCTC CCCCAGTGGC
    CTGCGGCCCA GAGGCTACAC CATCTCTGAT TCAGCCCCGT CACGCAGAGG CAAGAGGGTA
    GAGCGGGACG CCTTCAGGAG CAGAGCTGGG GCTTCCAACA CTGAGAAGGT GCCAGGCATC
    AATCCCAGCT TTGTGTTCCT GCAGCTCTAC CACTCACCCT TCTTTGGTGA CGAGTCCAAC
    AAGCCCATCC TTCTACCCAA TGAGTCCTTC GAGCGGTCAG TGCAGCTTCT CGACCAGATT
    CCATCATACG ACACACACAA GATTGCCGTC CTGTACGTGG GAGAAGGCCA GAGCAGCAGT
    GAGCTCGCCA TCCTGTCCAA TGAGCATGGC TCGTACAGGT ACACGGAGTT CCTGACAGGC
    CTGGGCAAGC TCATTGAGCT CAAAGACTGC CAGCCGGACA AGGTGTACCT GGGGGGCCTG
    GACGTGTGTG GCGAAGATGG CCAGTTCACC TACTGCTGGC ATGACGACAT CATGCAAGCC
    GTCTTCCACA TTGCTACCCT GATGCCCACC AAGGAAGTGG ACAAGCACCG CTGTGACAAG
    AAGCGCCACC TGGGCAATGA CTTTGTGTCC ATCGTCTACA ACGACTCTGG TGAGGACTTC
    AAGCTGGGCA CCATCCGAGG CCAGTTCAAC TTCGTCCACG TGATCATCAC CCCCCTGGAC
    TATGAGTGCA ACCTGGTGTC CCTGCAGTGC AGGAAAGACA TGGAAGGTCT TGTGGATACT
    AGCGTGGCCA AGATCGTGTC TGACCGCAAC TTGCCTTTTG TGGCACGTCA GATGGCTTTG
    CATGCAAATA TGGCCTCACA AGTGCATCAT AGCCGCTCCA ACCCCACCGA CATCTATCCC
    TCCAAGTGGA TTGCCAGGCT TCGCCACATC AAGCGACTCC GCCACCGGAT CCGCGAGGAA
    GCCCACTACA CCAACCCCAG CCTGCCACTG ATGCAGATGC ACCCCCCGGG CCACCCCAAA
    GCCTCTGCTC AGGCGCCGGC AGAGCCTGTG CCCACGTATG AGACAGGCCA GCGGAAGCGT
    CTCATCTCCT CTGTGGATGA CTTCACGGAG TTTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe08329
    Clone ID Related Accession (Same CDS sequence) XM_005621716.2 , XM_005621716.3
    Accession Version XM_005621716.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5439bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product tuberin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005621716.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
    CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC
    ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG
    ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG
    CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA
    GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG
    GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG
    GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG
    GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT
    GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC
    ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC
    GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG
    CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG
    GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG
    ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG
    CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC
    TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC
    CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC
    AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC
    CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG
    TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC
    TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT
    GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA
    GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT
    CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG
    AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC
    AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT
    AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG
    CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC
    AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT
    GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG
    CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG
    GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG
    GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG
    CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC
    GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG
    GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC
    GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC
    ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC
    GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG
    TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC
    CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC
    CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC
    AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT
    CCACCCGTGA AAGAATTCAA AGAGAGCTCT GCCGCCGAGG CCTTCCGATG CCGCAGCATC
    AGTGTGTCTG AACATGTGGT CCGCAGGATC CAGACGTCCC TCACAAGCGC CAGCTTGGGG
    TCTGCTGACG AGAACTCGAT GGCCCAGGCT GATGACAACT TGAAAAATCT CCACCTGGAG
    CTCACGGAAA CATGTCTGGA CATGATGGCC AGATATGTGT TCTCCAACTT CACAGCAGTC
    CCCAAGAGGT CCCCGGTGGG AGAGTTCCTC CTGGCTGGTG GCAGGACCAA AACCTGGCTG
    GTTGGGAACA AGCTTGTCAC TGTGACGACG AGCGTGGGGA CGGGGACCCG GTCGCTGCTG
    GGCTTGGACT CAGGAGATCA GCAAGGTGGC CCCGAGTTGA GCTCGGACCC TGGCATGCAT
    GTGAGACAGG CAAAGGAGGC ACCTGCCAAG CTGGAGTCGC AGGCTGGGCA GCAGGTGTGC
    CGCGGCGCCC GGGACCGGGT CCGCTCCATG TCCGGGGGCC ACGGTCTTCG TGTTGGCGCC
    CTGGACACTC CAGCCTCCCA CTTGCCGGGT GGCCCCACTT CCCCAGGCAC GCAGGCAGCG
    CTGGCCAGCA AGTCTGACAG GGCCTCGGCT GGCACCCGGT TCCCAGCACA GGAGAAGACG
    AGCCTGGCAG CCTACGTACC GCTGCTGACC CAGGGCTGGG CGGAGATCTT GGTCCGGAGG
    CCTACAGGGA ACACCAGCTG GCTGATGAGC CTGGAGAACC CGCTCAGCCC CTTCTCGTCG
    GACATCAACA ACATGCCTCT GCAGGAGCTG TCCAACGCGC TCATGGCCGC TGAGCGCTTC
    AAGGAGCGTC GTGACACGGC TCTGTACAAG TCGCTGTCGG TGCCTGCCGC AGGCTCGGCC
    AAGCCCCCTC CGCCCCCGAG ATCTAACACG GTGGCCTCTT TCTCCTCCCT GTACCAGTCC
    AGTTGCCAAG GAAAGCTGCA CAGGAGCATT TCCTGGGCAG ACTCTGCGGT GGTTCTGGAG
    GAGGGAAATT CAAGCGAGAC CAGTCTGCCA GTGGAGCACA CGGAGCTGGA GGACTTCGAG
    GCGACACTAG GCACAGACCG GCGCTGTGGC CGCGCGGAAG CTTACAGCAG GTCGTCTTCA
    AGCTCCAGCC AGGAGGAGAA GTTCCCTGCA GAGGAGCTGG CTGCCGGAGG GATCCCCATC
    GAACGAGCTG TCTCTTCCGA GGGTGCCCGG CCTTCTGTGG AGCTCTCCTT CCAGCCCTCC
    CAGCCCCTCA GCAAGTCCAG CTCCTCCCCT GAGCTGCAGA CCCTGCAGGA CATACTCGGG
    GACCCCGGGG ACAAGTCTGA TGTTGGCCGG CTCAGCCCTG AGGCCAAGGC CCGGTCACAG
    TCCGGGATTC TGGACGGGGT GGGTGCCTCC TGGTCAGCCC CAGGTGAAGA GAACCAGGGC
    CAGGGCCCCG CACAGCCCGA GGGTCCCTTG CCTTCCAGCT GCCCCCGCTC CCCCAGTGGC
    CTGCGGCCCA GAGGCTACAC CATCTCTGAT TCAGCCCCGT CACGCAGAGG CAAGAGGGTA
    GAGCGGGACG CCTTCAGGAG CAGAGCTGGG GCTTCCAACA CTGAGAAGGT GCCAGGCATC
    AATCCCAGCT TTGTGTTCCT GCAGCTCTAC CACTCACCCT TCTTTGGTGA CGAGTCCAAC
    AAGCCCATCC TTCTACCCAA TGAGTCCTTC GAGCGGTCAG TGCAGCTTCT CGACCAGATT
    CCATCATACG ACACACACAA GATTGCCGTC CTGTACGTGG GAGAAGGCCA GAGCAGCAGT
    GAGCTCGCCA TCCTGTCCAA TGAGCATGGC TCGTACAGGT ACACGGAGTT CCTGACAGGC
    CTGGGCAAGC TCATTGAGCT CAAAGACTGC CAGCCGGACA AGGTGTACCT GGGGGGCCTG
    GACGTGTGTG GCGAAGATGG CCAGTTCACC TACTGCTGGC ATGACGACAT CATGCAAGCC
    GTCTTCCACA TTGCTACCCT GATGCCCACC AAGGAAGTGG ACAAGCACCG CTGTGACAAG
    AAGCGCCACC TGGGCAATGA CTTTGTGTCC ATCGTCTACA ACGACTCTGG TGAGGACTTC
    AAGCTGGGCA CCATCCGAGG CCAGTTCAAC TTCGTCCACG TGATCATCAC CCCCCTGGAC
    TATGAGTGCA ACCTGGTGTC CCTGCAGTGC AGGAAAGACA TGGAAGGTCT TGTGGATACT
    AGCGTGGCCA AGATCGTGTC TGACCGCAAC TTGCCTTTTG TGGCACGTCA GATGGCTTTG
    CATGCAAATA TGGCCTCACA AGTGCATCAT AGCCGCTCCA ACCCCACCGA CATCTATCCC
    TCCAAGTGGA TTGCCAGGCT TCGCCACATC AAGCGACTCC GCCACCGGAT CCGCGAGGAA
    GCCCACTACA CCAACCCCAG CCTGCCACTG ATGCAGATGC ACCCCCCGGG CCACCCCAAA
    GCCTCTGCTC AGGCGCCGGC AGAGCCTGTG CCCACGTATG AGACAGGCCA GCGGAAGCGT
    CTCATCTCCT CTGTGGATGA CTTCACGGAG TTTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005621773.1
    CDS290..5728
    Translation

    Target ORF information:

    RefSeq Version XM_005621716.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005621716.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
    CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC
    ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG
    ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG
    CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA
    GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG
    GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG
    GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG
    GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT
    GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC
    ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC
    GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG
    CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG
    GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG
    ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG
    CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC
    TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC
    CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC
    AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC
    CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG
    TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC
    TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT
    GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA
    GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT
    CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG
    AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC
    AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT
    AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG
    CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC
    AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT
    GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG
    CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG
    GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG
    GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG
    CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC
    GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG
    GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC
    GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC
    ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC
    GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG
    TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC
    CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC
    CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC
    AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT
    CCACCCGTGA AAGAATTCAA AGAGAGCTCT GCCGCCGAGG CCTTCCGATG CCGCAGCATC
    AGTGTGTCTG AACATGTGGT CCGCAGGATC CAGACGTCCC TCACAAGCGC CAGCTTGGGG
    TCTGCTGACG AGAACTCGAT GGCCCAGGCT GATGACAACT TGAAAAATCT CCACCTGGAG
    CTCACGGAAA CATGTCTGGA CATGATGGCC AGATATGTGT TCTCCAACTT CACAGCAGTC
    CCCAAGAGGT CCCCGGTGGG AGAGTTCCTC CTGGCTGGTG GCAGGACCAA AACCTGGCTG
    GTTGGGAACA AGCTTGTCAC TGTGACGACG AGCGTGGGGA CGGGGACCCG GTCGCTGCTG
    GGCTTGGACT CAGGAGATCA GCAAGGTGGC CCCGAGTTGA GCTCGGACCC TGGCATGCAT
    GTGAGACAGG CAAAGGAGGC ACCTGCCAAG CTGGAGTCGC AGGCTGGGCA GCAGGTGTGC
    CGCGGCGCCC GGGACCGGGT CCGCTCCATG TCCGGGGGCC ACGGTCTTCG TGTTGGCGCC
    CTGGACACTC CAGCCTCCCA CTTGCCGGGT GGCCCCACTT CCCCAGGCAC GCAGGCAGCG
    CTGGCCAGCA AGTCTGACAG GGCCTCGGCT GGCACCCGGT TCCCAGCACA GGAGAAGACG
    AGCCTGGCAG CCTACGTACC GCTGCTGACC CAGGGCTGGG CGGAGATCTT GGTCCGGAGG
    CCTACAGGGA ACACCAGCTG GCTGATGAGC CTGGAGAACC CGCTCAGCCC CTTCTCGTCG
    GACATCAACA ACATGCCTCT GCAGGAGCTG TCCAACGCGC TCATGGCCGC TGAGCGCTTC
    AAGGAGCGTC GTGACACGGC TCTGTACAAG TCGCTGTCGG TGCCTGCCGC AGGCTCGGCC
    AAGCCCCCTC CGCCCCCGAG ATCTAACACG GTGGCCTCTT TCTCCTCCCT GTACCAGTCC
    AGTTGCCAAG GAAAGCTGCA CAGGAGCATT TCCTGGGCAG ACTCTGCGGT GGTTCTGGAG
    GAGGGAAATT CAAGCGAGAC CAGTCTGCCA GTGGAGCACA CGGAGCTGGA GGACTTCGAG
    GCGACACTAG GCACAGACCG GCGCTGTGGC CGCGCGGAAG CTTACAGCAG GTCGTCTTCA
    AGCTCCAGCC AGGAGGAGAA GTTCCCTGCA GAGGAGCTGG CTGCCGGAGG GATCCCCATC
    GAACGAGCTG TCTCTTCCGA GGGTGCCCGG CCTTCTGTGG AGCTCTCCTT CCAGCCCTCC
    CAGCCCCTCA GCAAGTCCAG CTCCTCCCCT GAGCTGCAGA CCCTGCAGGA CATACTCGGG
    GACCCCGGGG ACAAGTCTGA TGTTGGCCGG CTCAGCCCTG AGGCCAAGGC CCGGTCACAG
    TCCGGGATTC TGGACGGGGT GGGTGCCTCC TGGTCAGCCC CAGGTGAAGA GAACCAGGGC
    CAGGGCCCCG CACAGCCCGA GGGTCCCTTG CCTTCCAGCT GCCCCCGCTC CCCCAGTGGC
    CTGCGGCCCA GAGGCTACAC CATCTCTGAT TCAGCCCCGT CACGCAGAGG CAAGAGGGTA
    GAGCGGGACG CCTTCAGGAG CAGAGCTGGG GCTTCCAACA CTGAGAAGGT GCCAGGCATC
    AATCCCAGCT TTGTGTTCCT GCAGCTCTAC CACTCACCCT TCTTTGGTGA CGAGTCCAAC
    AAGCCCATCC TTCTACCCAA TGAGTCCTTC GAGCGGTCAG TGCAGCTTCT CGACCAGATT
    CCATCATACG ACACACACAA GATTGCCGTC CTGTACGTGG GAGAAGGCCA GAGCAGCAGT
    GAGCTCGCCA TCCTGTCCAA TGAGCATGGC TCGTACAGGT ACACGGAGTT CCTGACAGGC
    CTGGGCAAGC TCATTGAGCT CAAAGACTGC CAGCCGGACA AGGTGTACCT GGGGGGCCTG
    GACGTGTGTG GCGAAGATGG CCAGTTCACC TACTGCTGGC ATGACGACAT CATGCAAGCC
    GTCTTCCACA TTGCTACCCT GATGCCCACC AAGGAAGTGG ACAAGCACCG CTGTGACAAG
    AAGCGCCACC TGGGCAATGA CTTTGTGTCC ATCGTCTACA ACGACTCTGG TGAGGACTTC
    AAGCTGGGCA CCATCCGAGG CCAGTTCAAC TTCGTCCACG TGATCATCAC CCCCCTGGAC
    TATGAGTGCA ACCTGGTGTC CCTGCAGTGC AGGAAAGACA TGGAAGGTCT TGTGGATACT
    AGCGTGGCCA AGATCGTGTC TGACCGCAAC TTGCCTTTTG TGGCACGTCA GATGGCTTTG
    CATGCAAATA TGGCCTCACA AGTGCATCAT AGCCGCTCCA ACCCCACCGA CATCTATCCC
    TCCAAGTGGA TTGCCAGGCT TCGCCACATC AAGCGACTCC GCCACCGGAT CCGCGAGGAA
    GCCCACTACA CCAACCCCAG CCTGCCACTG ATGCAGATGC ACCCCCCGGG CCACCCCAAA
    GCCTCTGCTC AGGCGCCGGC AGAGCCTGTG CCCACGTATG AGACAGGCCA GCGGAAGCGT
    CTCATCTCCT CTGTGGATGA CTTCACGGAG TTTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe08331
    Clone ID Related Accession (Same CDS sequence) XM_005621717.2 , XM_005621717.3
    Accession Version XM_005621717.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5373bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product tuberin isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005621717.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
    CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC
    ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG
    ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG
    CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA
    GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG
    GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG
    GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG
    GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT
    GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC
    ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC
    GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG
    CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG
    GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG
    ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG
    CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC
    TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC
    CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC
    AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC
    CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG
    TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC
    TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT
    GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA
    GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT
    CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG
    AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC
    AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT
    AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG
    CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC
    AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT
    GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG
    CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG
    GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG
    GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG
    CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC
    GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG
    GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC
    GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC
    ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC
    GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG
    TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC
    CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC
    CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC
    AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT
    CCACCCGTGA AAGAATTCAA AGAGAGCTCT GCCGCCGAGG CCTTCCGATG CCGCAGCATC
    AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATCCAGACGT CCCTCACAAG CGCCAGCTTG
    GGGTCTGCTG ACGAGAACTC GATGGCCCAG GCTGATGACA ACTTGAAAAA TCTCCACCTG
    GAGCTCACGG AAACATGTCT GGACATGATG GCCAGATATG TGTTCTCCAA CTTCACAGCA
    GTCCCCAAGA GGTCCCCGGT GGGAGAGTTC CTCCTGGCTG GTGGCAGGAC CAAAACCTGG
    CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACGAGCGTGG GGACGGGGAC CCGGTCGCTG
    CTGGGCTTGG ACTCAGGAGA TCAGCAAGGT GGCCCCGAGT TGAGCTCGGA CCCTGGCATG
    CATGTGAGAC AGGCAAAGGA GGCACCTGCC AAGCTGGAGT CGCAGGCTGG GCAGCAGGTG
    TGCCGCGGCG CCCGGGACCG GGTCCGCTCC ATGTCCGGGG GCCACGGTCT TCGTGTTGGC
    GCCCTGGACA CTCCAGCCTC CCACTTGCCG GGTGGCCCCA CTTCCCCAGG CACGCAGGCA
    GCGCTGGCCA GCAAGTCTGA CAGGGCCTCG GCTGGCACCC GGTTCCCAGC ACAGGAGAAG
    ACGAGCCTGG CAGCCTACGT ACCGCTGCTG ACCCAGGGCT GGGCGGAGAT CTTGGTCCGG
    AGGCCTACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCCTTCTCG
    TCGGACATCA ACAACATGCC TCTGCAGGAG CTGTCCAACG CGCTCATGGC CGCTGAGCGC
    TTCAAGGAGC GTCGTGACAC GGCTCTGTAC AAGTCGCTGT CGGTGCCTGC CGCAGGCTCG
    GCCAAGCCCC CTCCGCCCCC GAGATCTAAC ACGGACTCTG CGGTGGTTCT GGAGGAGGGA
    AATTCAAGCG AGACCAGTCT GCCAGTGGAG CACACGGAGC TGGAGGACTT CGAGGCGACA
    CTAGGCACAG ACCGGCGCTG TGGCCGCGCG GAAGCTTACA GCAGGTCGTC TTCAAGCTCC
    AGCCAGGAGG AGAAGTTCCC TGCAGAGGAG CTGGCTGCCG GAGGGATCCC CATCGAACGA
    GCTGTCTCTT CCGAGGGTGC CCGGCCTTCT GTGGAGCTCT CCTTCCAGCC CTCCCAGCCC
    CTCAGCAAGT CCAGCTCCTC CCCTGAGCTG CAGACCCTGC AGGACATACT CGGGGACCCC
    GGGGACAAGT CTGATGTTGG CCGGCTCAGC CCTGAGGCCA AGGCCCGGTC ACAGTCCGGG
    ATTCTGGACG GGGTGGGTGC CTCCTGGTCA GCCCCAGGTG AAGAGAACCA GGGCCAGGGC
    CCCGCACAGC CCGAGGGTCC CTTGCCTTCC AGCTGCCCCC GCTCCCCCAG TGGCCTGCGG
    CCCAGAGGCT ACACCATCTC TGATTCAGCC CCGTCACGCA GAGGCAAGAG GGTAGAGCGG
    GACGCCTTCA GGAGCAGAGC TGGGGCTTCC AACACTGAGA AGGTGCCAGG CATCAATCCC
    AGCTTTGTGT TCCTGCAGCT CTACCACTCA CCCTTCTTTG GTGACGAGTC CAACAAGCCC
    ATCCTTCTAC CCAATGAGTC CTTCGAGCGG TCAGTGCAGC TTCTCGACCA GATTCCATCA
    TACGACACAC ACAAGATTGC CGTCCTGTAC GTGGGAGAAG GCCAGAGCAG CAGTGAGCTC
    GCCATCCTGT CCAATGAGCA TGGCTCGTAC AGGTACACGG AGTTCCTGAC AGGCCTGGGC
    AAGCTCATTG AGCTCAAAGA CTGCCAGCCG GACAAGGTGT ACCTGGGGGG CCTGGACGTG
    TGTGGCGAAG ATGGCCAGTT CACCTACTGC TGGCATGACG ACATCATGCA AGCCGTCTTC
    CACATTGCTA CCCTGATGCC CACCAAGGAA GTGGACAAGC ACCGCTGTGA CAAGAAGCGC
    CACCTGGGCA ATGACTTTGT GTCCATCGTC TACAACGACT CTGGTGAGGA CTTCAAGCTG
    GGCACCATCC GAGGCCAGTT CAACTTCGTC CACGTGATCA TCACCCCCCT GGACTATGAG
    TGCAACCTGG TGTCCCTGCA GTGCAGGAAA GACATGGAAG GTCTTGTGGA TACTAGCGTG
    GCCAAGATCG TGTCTGACCG CAACTTGCCT TTTGTGGCAC GTCAGATGGC TTTGCATGCA
    AATATGGCCT CACAAGTGCA TCATAGCCGC TCCAACCCCA CCGACATCTA TCCCTCCAAG
    TGGATTGCCA GGCTTCGCCA CATCAAGCGA CTCCGCCACC GGATCCGCGA GGAAGCCCAC
    TACACCAACC CCAGCCTGCC ACTGATGCAG ATGCACCCCC CGGGCCACCC CAAAGCCTCT
    GCTCAGGCGC CGGCAGAGCC TGTGCCCACG TATGAGACAG GCCAGCGGAA GCGTCTCATC
    TCCTCTGTGG ATGACTTCAC GGAGTTTGTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005621774.1
    CDS283..5655
    Translation

    Target ORF information:

    RefSeq Version XM_005621717.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris tuberous sclerosis 2 (TSC2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005621717.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
    CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC
    ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG
    ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG
    CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA
    GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG
    GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG
    GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG
    GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT
    GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC
    ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC
    GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG
    CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG
    GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG
    ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG
    CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC
    TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC
    CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC
    AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC
    CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG
    TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC
    TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT
    GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA
    GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT
    CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG
    AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC
    AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT
    AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG
    CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC
    AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT
    GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG
    CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG
    GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG
    GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG
    CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC
    GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG
    GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC
    GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC
    ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC
    GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG
    TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC
    CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC
    CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC
    AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT
    CCACCCGTGA AAGAATTCAA AGAGAGCTCT GCCGCCGAGG CCTTCCGATG CCGCAGCATC
    AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATCCAGACGT CCCTCACAAG CGCCAGCTTG
    GGGTCTGCTG ACGAGAACTC GATGGCCCAG GCTGATGACA ACTTGAAAAA TCTCCACCTG
    GAGCTCACGG AAACATGTCT GGACATGATG GCCAGATATG TGTTCTCCAA CTTCACAGCA
    GTCCCCAAGA GGTCCCCGGT GGGAGAGTTC CTCCTGGCTG GTGGCAGGAC CAAAACCTGG
    CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACGAGCGTGG GGACGGGGAC CCGGTCGCTG
    CTGGGCTTGG ACTCAGGAGA TCAGCAAGGT GGCCCCGAGT TGAGCTCGGA CCCTGGCATG
    CATGTGAGAC AGGCAAAGGA GGCACCTGCC AAGCTGGAGT CGCAGGCTGG GCAGCAGGTG
    TGCCGCGGCG CCCGGGACCG GGTCCGCTCC ATGTCCGGGG GCCACGGTCT TCGTGTTGGC
    GCCCTGGACA CTCCAGCCTC CCACTTGCCG GGTGGCCCCA CTTCCCCAGG CACGCAGGCA
    GCGCTGGCCA GCAAGTCTGA CAGGGCCTCG GCTGGCACCC GGTTCCCAGC ACAGGAGAAG
    ACGAGCCTGG CAGCCTACGT ACCGCTGCTG ACCCAGGGCT GGGCGGAGAT CTTGGTCCGG
    AGGCCTACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCCTTCTCG
    TCGGACATCA ACAACATGCC TCTGCAGGAG CTGTCCAACG CGCTCATGGC CGCTGAGCGC
    TTCAAGGAGC GTCGTGACAC GGCTCTGTAC AAGTCGCTGT CGGTGCCTGC CGCAGGCTCG
    GCCAAGCCCC CTCCGCCCCC GAGATCTAAC ACGGACTCTG CGGTGGTTCT GGAGGAGGGA
    AATTCAAGCG AGACCAGTCT GCCAGTGGAG CACACGGAGC TGGAGGACTT CGAGGCGACA
    CTAGGCACAG ACCGGCGCTG TGGCCGCGCG GAAGCTTACA GCAGGTCGTC TTCAAGCTCC
    AGCCAGGAGG AGAAGTTCCC TGCAGAGGAG CTGGCTGCCG GAGGGATCCC CATCGAACGA
    GCTGTCTCTT CCGAGGGTGC CCGGCCTTCT GTGGAGCTCT CCTTCCAGCC CTCCCAGCCC
    CTCAGCAAGT CCAGCTCCTC CCCTGAGCTG CAGACCCTGC AGGACATACT CGGGGACCCC
    GGGGACAAGT CTGATGTTGG CCGGCTCAGC CCTGAGGCCA AGGCCCGGTC ACAGTCCGGG
    ATTCTGGACG GGGTGGGTGC CTCCTGGTCA GCCCCAGGTG AAGAGAACCA GGGCCAGGGC
    CCCGCACAGC CCGAGGGTCC CTTGCCTTCC AGCTGCCCCC GCTCCCCCAG TGGCCTGCGG
    CCCAGAGGCT ACACCATCTC TGATTCAGCC CCGTCACGCA GAGGCAAGAG GGTAGAGCGG
    GACGCCTTCA GGAGCAGAGC TGGGGCTTCC AACACTGAGA AGGTGCCAGG CATCAATCCC
    AGCTTTGTGT TCCTGCAGCT CTACCACTCA CCCTTCTTTG GTGACGAGTC CAACAAGCCC
    ATCCTTCTAC CCAATGAGTC CTTCGAGCGG TCAGTGCAGC TTCTCGACCA GATTCCATCA
    TACGACACAC ACAAGATTGC CGTCCTGTAC GTGGGAGAAG GCCAGAGCAG CAGTGAGCTC
    GCCATCCTGT CCAATGAGCA TGGCTCGTAC AGGTACACGG AGTTCCTGAC AGGCCTGGGC
    AAGCTCATTG AGCTCAAAGA CTGCCAGCCG GACAAGGTGT ACCTGGGGGG CCTGGACGTG
    TGTGGCGAAG ATGGCCAGTT CACCTACTGC TGGCATGACG ACATCATGCA AGCCGTCTTC
    CACATTGCTA CCCTGATGCC CACCAAGGAA GTGGACAAGC ACCGCTGTGA CAAGAAGCGC
    CACCTGGGCA ATGACTTTGT GTCCATCGTC TACAACGACT CTGGTGAGGA CTTCAAGCTG
    GGCACCATCC GAGGCCAGTT CAACTTCGTC CACGTGATCA TCACCCCCCT GGACTATGAG
    TGCAACCTGG TGTCCCTGCA GTGCAGGAAA GACATGGAAG GTCTTGTGGA TACTAGCGTG
    GCCAAGATCG TGTCTGACCG CAACTTGCCT TTTGTGGCAC GTCAGATGGC TTTGCATGCA
    AATATGGCCT CACAAGTGCA TCATAGCCGC TCCAACCCCA CCGACATCTA TCCCTCCAAG
    TGGATTGCCA GGCTTCGCCA CATCAAGCGA CTCCGCCACC GGATCCGCGA GGAAGCCCAC
    TACACCAACC CCAGCCTGCC ACTGATGCAG ATGCACCCCC CGGGCCACCC CAAAGCCTCT
    GCTCAGGCGC CGGCAGAGCC TGTGCCCACG TATGAGACAG GCCAGCGGAA GCGTCTCATC
    TCCTCTGTGG ATGACTTCAC GGAGTTTGTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe08331
    Clone ID Related Accession (Same CDS sequence) XM_005621717.2 , XM_005621717.3
    Accession Version XM_005621717.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5373bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product tuberin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005621717.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
    CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC
    ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG
    ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG
    CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA
    GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG
    GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG
    GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG
    GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT
    GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC
    ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC
    GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG
    CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG
    GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG
    ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG
    CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC
    TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC
    CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC
    AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC
    CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG
    TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC
    TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT
    GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA
    GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT
    CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG
    AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC
    AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT
    AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG
    CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC
    AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT
    GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG
    CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG
    GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG
    GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG
    CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC
    GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG
    GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC
    GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC
    ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC
    GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG
    TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC
    CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC
    CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC
    AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT
    CCACCCGTGA AAGAATTCAA AGAGAGCTCT GCCGCCGAGG CCTTCCGATG CCGCAGCATC
    AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATCCAGACGT CCCTCACAAG CGCCAGCTTG
    GGGTCTGCTG ACGAGAACTC GATGGCCCAG GCTGATGACA ACTTGAAAAA TCTCCACCTG
    GAGCTCACGG AAACATGTCT GGACATGATG GCCAGATATG TGTTCTCCAA CTTCACAGCA
    GTCCCCAAGA GGTCCCCGGT GGGAGAGTTC CTCCTGGCTG GTGGCAGGAC CAAAACCTGG
    CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACGAGCGTGG GGACGGGGAC CCGGTCGCTG
    CTGGGCTTGG ACTCAGGAGA TCAGCAAGGT GGCCCCGAGT TGAGCTCGGA CCCTGGCATG
    CATGTGAGAC AGGCAAAGGA GGCACCTGCC AAGCTGGAGT CGCAGGCTGG GCAGCAGGTG
    TGCCGCGGCG CCCGGGACCG GGTCCGCTCC ATGTCCGGGG GCCACGGTCT TCGTGTTGGC
    GCCCTGGACA CTCCAGCCTC CCACTTGCCG GGTGGCCCCA CTTCCCCAGG CACGCAGGCA
    GCGCTGGCCA GCAAGTCTGA CAGGGCCTCG GCTGGCACCC GGTTCCCAGC ACAGGAGAAG
    ACGAGCCTGG CAGCCTACGT ACCGCTGCTG ACCCAGGGCT GGGCGGAGAT CTTGGTCCGG
    AGGCCTACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCCTTCTCG
    TCGGACATCA ACAACATGCC TCTGCAGGAG CTGTCCAACG CGCTCATGGC CGCTGAGCGC
    TTCAAGGAGC GTCGTGACAC GGCTCTGTAC AAGTCGCTGT CGGTGCCTGC CGCAGGCTCG
    GCCAAGCCCC CTCCGCCCCC GAGATCTAAC ACGGACTCTG CGGTGGTTCT GGAGGAGGGA
    AATTCAAGCG AGACCAGTCT GCCAGTGGAG CACACGGAGC TGGAGGACTT CGAGGCGACA
    CTAGGCACAG ACCGGCGCTG TGGCCGCGCG GAAGCTTACA GCAGGTCGTC TTCAAGCTCC
    AGCCAGGAGG AGAAGTTCCC TGCAGAGGAG CTGGCTGCCG GAGGGATCCC CATCGAACGA
    GCTGTCTCTT CCGAGGGTGC CCGGCCTTCT GTGGAGCTCT CCTTCCAGCC CTCCCAGCCC
    CTCAGCAAGT CCAGCTCCTC CCCTGAGCTG CAGACCCTGC AGGACATACT CGGGGACCCC
    GGGGACAAGT CTGATGTTGG CCGGCTCAGC CCTGAGGCCA AGGCCCGGTC ACAGTCCGGG
    ATTCTGGACG GGGTGGGTGC CTCCTGGTCA GCCCCAGGTG AAGAGAACCA GGGCCAGGGC
    CCCGCACAGC CCGAGGGTCC CTTGCCTTCC AGCTGCCCCC GCTCCCCCAG TGGCCTGCGG
    CCCAGAGGCT ACACCATCTC TGATTCAGCC CCGTCACGCA GAGGCAAGAG GGTAGAGCGG
    GACGCCTTCA GGAGCAGAGC TGGGGCTTCC AACACTGAGA AGGTGCCAGG CATCAATCCC
    AGCTTTGTGT TCCTGCAGCT CTACCACTCA CCCTTCTTTG GTGACGAGTC CAACAAGCCC
    ATCCTTCTAC CCAATGAGTC CTTCGAGCGG TCAGTGCAGC TTCTCGACCA GATTCCATCA
    TACGACACAC ACAAGATTGC CGTCCTGTAC GTGGGAGAAG GCCAGAGCAG CAGTGAGCTC
    GCCATCCTGT CCAATGAGCA TGGCTCGTAC AGGTACACGG AGTTCCTGAC AGGCCTGGGC
    AAGCTCATTG AGCTCAAAGA CTGCCAGCCG GACAAGGTGT ACCTGGGGGG CCTGGACGTG
    TGTGGCGAAG ATGGCCAGTT CACCTACTGC TGGCATGACG ACATCATGCA AGCCGTCTTC
    CACATTGCTA CCCTGATGCC CACCAAGGAA GTGGACAAGC ACCGCTGTGA CAAGAAGCGC
    CACCTGGGCA ATGACTTTGT GTCCATCGTC TACAACGACT CTGGTGAGGA CTTCAAGCTG
    GGCACCATCC GAGGCCAGTT CAACTTCGTC CACGTGATCA TCACCCCCCT GGACTATGAG
    TGCAACCTGG TGTCCCTGCA GTGCAGGAAA GACATGGAAG GTCTTGTGGA TACTAGCGTG
    GCCAAGATCG TGTCTGACCG CAACTTGCCT TTTGTGGCAC GTCAGATGGC TTTGCATGCA
    AATATGGCCT CACAAGTGCA TCATAGCCGC TCCAACCCCA CCGACATCTA TCCCTCCAAG
    TGGATTGCCA GGCTTCGCCA CATCAAGCGA CTCCGCCACC GGATCCGCGA GGAAGCCCAC
    TACACCAACC CCAGCCTGCC ACTGATGCAG ATGCACCCCC CGGGCCACCC CAAAGCCTCT
    GCTCAGGCGC CGGCAGAGCC TGTGCCCACG TATGAGACAG GCCAGCGGAA GCGTCTCATC
    TCCTCTGTGG ATGACTTCAC GGAGTTTGTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005621774.1
    CDS289..5661
    Translation

    Target ORF information:

    RefSeq Version XM_005621717.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005621717.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
    CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC
    ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG
    ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG
    CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA
    GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG
    GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG
    GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG
    GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT
    GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC
    ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC
    GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG
    CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG
    GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG
    ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG
    CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC
    TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC
    CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC
    AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC
    CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG
    TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC
    TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT
    GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA
    GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT
    CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG
    AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC
    AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT
    AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG
    CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC
    AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT
    GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG
    CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG
    GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG
    GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG
    CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC
    GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG
    GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC
    GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC
    ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC
    GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG
    TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC
    CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC
    CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC
    AACGAGAGGC CCAAGAGTTT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT
    CCACCCGTGA AAGAATTCAA AGAGAGCTCT GCCGCCGAGG CCTTCCGATG CCGCAGCATC
    AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATCCAGACGT CCCTCACAAG CGCCAGCTTG
    GGGTCTGCTG ACGAGAACTC GATGGCCCAG GCTGATGACA ACTTGAAAAA TCTCCACCTG
    GAGCTCACGG AAACATGTCT GGACATGATG GCCAGATATG TGTTCTCCAA CTTCACAGCA
    GTCCCCAAGA GGTCCCCGGT GGGAGAGTTC CTCCTGGCTG GTGGCAGGAC CAAAACCTGG
    CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACGAGCGTGG GGACGGGGAC CCGGTCGCTG
    CTGGGCTTGG ACTCAGGAGA TCAGCAAGGT GGCCCCGAGT TGAGCTCGGA CCCTGGCATG
    CATGTGAGAC AGGCAAAGGA GGCACCTGCC AAGCTGGAGT CGCAGGCTGG GCAGCAGGTG
    TGCCGCGGCG CCCGGGACCG GGTCCGCTCC ATGTCCGGGG GCCACGGTCT TCGTGTTGGC
    GCCCTGGACA CTCCAGCCTC CCACTTGCCG GGTGGCCCCA CTTCCCCAGG CACGCAGGCA
    GCGCTGGCCA GCAAGTCTGA CAGGGCCTCG GCTGGCACCC GGTTCCCAGC ACAGGAGAAG
    ACGAGCCTGG CAGCCTACGT ACCGCTGCTG ACCCAGGGCT GGGCGGAGAT CTTGGTCCGG
    AGGCCTACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCCTTCTCG
    TCGGACATCA ACAACATGCC TCTGCAGGAG CTGTCCAACG CGCTCATGGC CGCTGAGCGC
    TTCAAGGAGC GTCGTGACAC GGCTCTGTAC AAGTCGCTGT CGGTGCCTGC CGCAGGCTCG
    GCCAAGCCCC CTCCGCCCCC GAGATCTAAC ACGGACTCTG CGGTGGTTCT GGAGGAGGGA
    AATTCAAGCG AGACCAGTCT GCCAGTGGAG CACACGGAGC TGGAGGACTT CGAGGCGACA
    CTAGGCACAG ACCGGCGCTG TGGCCGCGCG GAAGCTTACA GCAGGTCGTC TTCAAGCTCC
    AGCCAGGAGG AGAAGTTCCC TGCAGAGGAG CTGGCTGCCG GAGGGATCCC CATCGAACGA
    GCTGTCTCTT CCGAGGGTGC CCGGCCTTCT GTGGAGCTCT CCTTCCAGCC CTCCCAGCCC
    CTCAGCAAGT CCAGCTCCTC CCCTGAGCTG CAGACCCTGC AGGACATACT CGGGGACCCC
    GGGGACAAGT CTGATGTTGG CCGGCTCAGC CCTGAGGCCA AGGCCCGGTC ACAGTCCGGG
    ATTCTGGACG GGGTGGGTGC CTCCTGGTCA GCCCCAGGTG AAGAGAACCA GGGCCAGGGC
    CCCGCACAGC CCGAGGGTCC CTTGCCTTCC AGCTGCCCCC GCTCCCCCAG TGGCCTGCGG
    CCCAGAGGCT ACACCATCTC TGATTCAGCC CCGTCACGCA GAGGCAAGAG GGTAGAGCGG
    GACGCCTTCA GGAGCAGAGC TGGGGCTTCC AACACTGAGA AGGTGCCAGG CATCAATCCC
    AGCTTTGTGT TCCTGCAGCT CTACCACTCA CCCTTCTTTG GTGACGAGTC CAACAAGCCC
    ATCCTTCTAC CCAATGAGTC CTTCGAGCGG TCAGTGCAGC TTCTCGACCA GATTCCATCA
    TACGACACAC ACAAGATTGC CGTCCTGTAC GTGGGAGAAG GCCAGAGCAG CAGTGAGCTC
    GCCATCCTGT CCAATGAGCA TGGCTCGTAC AGGTACACGG AGTTCCTGAC AGGCCTGGGC
    AAGCTCATTG AGCTCAAAGA CTGCCAGCCG GACAAGGTGT ACCTGGGGGG CCTGGACGTG
    TGTGGCGAAG ATGGCCAGTT CACCTACTGC TGGCATGACG ACATCATGCA AGCCGTCTTC
    CACATTGCTA CCCTGATGCC CACCAAGGAA GTGGACAAGC ACCGCTGTGA CAAGAAGCGC
    CACCTGGGCA ATGACTTTGT GTCCATCGTC TACAACGACT CTGGTGAGGA CTTCAAGCTG
    GGCACCATCC GAGGCCAGTT CAACTTCGTC CACGTGATCA TCACCCCCCT GGACTATGAG
    TGCAACCTGG TGTCCCTGCA GTGCAGGAAA GACATGGAAG GTCTTGTGGA TACTAGCGTG
    GCCAAGATCG TGTCTGACCG CAACTTGCCT TTTGTGGCAC GTCAGATGGC TTTGCATGCA
    AATATGGCCT CACAAGTGCA TCATAGCCGC TCCAACCCCA CCGACATCTA TCCCTCCAAG
    TGGATTGCCA GGCTTCGCCA CATCAAGCGA CTCCGCCACC GGATCCGCGA GGAAGCCCAC
    TACACCAACC CCAGCCTGCC ACTGATGCAG ATGCACCCCC CGGGCCACCC CAAAGCCTCT
    GCTCAGGCGC CGGCAGAGCC TGTGCCCACG TATGAGACAG GCCAGCGGAA GCGTCTCATC
    TCCTCTGTGG ATGACTTCAC GGAGTTTGTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe08333
    Clone ID Related Accession (Same CDS sequence) XM_005621718.2 , XM_005621718.3
    Accession Version XM_005621718.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5313bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product tuberin isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005621718.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
    CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC
    ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG
    ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG
    CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA
    GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG
    GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG
    GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG
    GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT
    GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC
    ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC
    GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG
    CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG
    GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG
    ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG
    CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC
    TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC
    CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC
    AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC
    CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG
    TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC
    TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT
    GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA
    GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT
    CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG
    AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC
    AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT
    AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG
    CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC
    AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT
    GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG
    CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG
    GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG
    GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG
    CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC
    GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG
    GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC
    GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC
    ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC
    GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG
    TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC
    CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC
    CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC
    AACGAGAGGC CCAAGAGCAG GATCCAGACG TCCCTCACAA GCGCCAGCTT GGGGTCTGCT
    GACGAGAACT CGATGGCCCA GGCTGATGAC AACTTGAAAA ATCTCCACCT GGAGCTCACG
    GAAACATGTC TGGACATGAT GGCCAGATAT GTGTTCTCCA ACTTCACAGC AGTCCCCAAG
    AGGTCCCCGG TGGGAGAGTT CCTCCTGGCT GGTGGCAGGA CCAAAACCTG GCTGGTTGGG
    AACAAGCTTG TCACTGTGAC GACGAGCGTG GGGACGGGGA CCCGGTCGCT GCTGGGCTTG
    GACTCAGGAG ATCAGCAAGG TGGCCCCGAG TTGAGCTCGG ACCCTGGCAT GCATGTGAGA
    CAGGCAAAGG AGGCACCTGC CAAGCTGGAG TCGCAGGCTG GGCAGCAGGT GTGCCGCGGC
    GCCCGGGACC GGGTCCGCTC CATGTCCGGG GGCCACGGTC TTCGTGTTGG CGCCCTGGAC
    ACTCCAGCCT CCCACTTGCC GGGTGGCCCC ACTTCCCCAG GCACGCAGGC AGCGCTGGCC
    AGCAAGTCTG ACAGGGCCTC GGCTGGCACC CGGTTCCCAG CACAGGAGAA GACGAGCCTG
    GCAGCCTACG TACCGCTGCT GACCCAGGGC TGGGCGGAGA TCTTGGTCCG GAGGCCTACA
    GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCCTTCTC GTCGGACATC
    AACAACATGC CTCTGCAGGA GCTGTCCAAC GCGCTCATGG CCGCTGAGCG CTTCAAGGAG
    CGTCGTGACA CGGCTCTGTA CAAGTCGCTG TCGGTGCCTG CCGCAGGCTC GGCCAAGCCC
    CCTCCGCCCC CGAGATCTAA CACGGTGGCC TCTTTCTCCT CCCTGTACCA GTCCAGTTGC
    CAAGGAAAGC TGCACAGGAG CATTTCCTGG GCAGACTCTG CGGTGGTTCT GGAGGAGGGA
    AATTCAAGCG AGACCAGTCT GCCAGTGGAG CACACGGAGC TGGAGGACTT CGAGGCGACA
    CTAGGCACAG ACCGGCGCTG TGGCCGCGCG GAAGCTTACA GCAGGTCGTC TTCAAGCTCC
    AGCCAGGAGG AGAAGTTCCC TGCAGAGGAG CTGGCTGCCG GAGGGATCCC CATCGAACGA
    GCTGTCTCTT CCGAGGGTGC CCGGCCTTCT GTGGAGCTCT CCTTCCAGCC CTCCCAGCCC
    CTCAGCAAGT CCAGCTCCTC CCCTGAGCTG CAGACCCTGC AGGACATACT CGGGGACCCC
    GGGGACAAGT CTGATGTTGG CCGGCTCAGC CCTGAGGCCA AGGCCCGGTC ACAGTCCGGG
    ATTCTGGACG GGGTGGGTGC CTCCTGGTCA GCCCCAGGTG AAGAGAACCA GGGCCAGGGC
    CCCGCACAGC CCGAGGGTCC CTTGCCTTCC AGCTGCCCCC GCTCCCCCAG TGGCCTGCGG
    CCCAGAGGCT ACACCATCTC TGATTCAGCC CCGTCACGCA GAGGCAAGAG GGTAGAGCGG
    GACGCCTTCA GGAGCAGAGC TGGGGCTTCC AACACTGAGA AGGTGCCAGG CATCAATCCC
    AGCTTTGTGT TCCTGCAGCT CTACCACTCA CCCTTCTTTG GTGACGAGTC CAACAAGCCC
    ATCCTTCTAC CCAATGAGTC CTTCGAGCGG TCAGTGCAGC TTCTCGACCA GATTCCATCA
    TACGACACAC ACAAGATTGC CGTCCTGTAC GTGGGAGAAG GCCAGAGCAG CAGTGAGCTC
    GCCATCCTGT CCAATGAGCA TGGCTCGTAC AGGTACACGG AGTTCCTGAC AGGCCTGGGC
    AAGCTCATTG AGCTCAAAGA CTGCCAGCCG GACAAGGTGT ACCTGGGGGG CCTGGACGTG
    TGTGGCGAAG ATGGCCAGTT CACCTACTGC TGGCATGACG ACATCATGCA AGCCGTCTTC
    CACATTGCTA CCCTGATGCC CACCAAGGAA GTGGACAAGC ACCGCTGTGA CAAGAAGCGC
    CACCTGGGCA ATGACTTTGT GTCCATCGTC TACAACGACT CTGGTGAGGA CTTCAAGCTG
    GGCACCATCC GAGGCCAGTT CAACTTCGTC CACGTGATCA TCACCCCCCT GGACTATGAG
    TGCAACCTGG TGTCCCTGCA GTGCAGGAAA GACATGGAAG GTCTTGTGGA TACTAGCGTG
    GCCAAGATCG TGTCTGACCG CAACTTGCCT TTTGTGGCAC GTCAGATGGC TTTGCATGCA
    AATATGGCCT CACAAGTGCA TCATAGCCGC TCCAACCCCA CCGACATCTA TCCCTCCAAG
    TGGATTGCCA GGCTTCGCCA CATCAAGCGA CTCCGCCACC GGATCCGCGA GGAAGCCCAC
    TACACCAACC CCAGCCTGCC ACTGATGCAG ATGCACCCCC CGGGCCACCC CAAAGCCTCT
    GCTCAGGCGC CGGCAGAGCC TGTGCCCACG TATGAGACAG GCCAGCGGAA GCGTCTCATC
    TCCTCTGTGG ATGACTTCAC GGAGTTTGTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005621775.1
    CDS283..5595
    Translation

    Target ORF information:

    RefSeq Version XM_005621718.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris tuberous sclerosis 2 (TSC2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005621718.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
    CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC
    ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG
    ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG
    CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA
    GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG
    GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG
    GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG
    GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT
    GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC
    ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC
    GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG
    CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG
    GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG
    ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG
    CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC
    TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC
    CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC
    AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC
    CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG
    TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC
    TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT
    GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA
    GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT
    CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG
    AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC
    AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT
    AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG
    CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC
    AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT
    GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG
    CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG
    GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG
    GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG
    CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC
    GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG
    GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC
    GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC
    ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC
    GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG
    TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC
    CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC
    CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC
    AACGAGAGGC CCAAGAGCAG GATCCAGACG TCCCTCACAA GCGCCAGCTT GGGGTCTGCT
    GACGAGAACT CGATGGCCCA GGCTGATGAC AACTTGAAAA ATCTCCACCT GGAGCTCACG
    GAAACATGTC TGGACATGAT GGCCAGATAT GTGTTCTCCA ACTTCACAGC AGTCCCCAAG
    AGGTCCCCGG TGGGAGAGTT CCTCCTGGCT GGTGGCAGGA CCAAAACCTG GCTGGTTGGG
    AACAAGCTTG TCACTGTGAC GACGAGCGTG GGGACGGGGA CCCGGTCGCT GCTGGGCTTG
    GACTCAGGAG ATCAGCAAGG TGGCCCCGAG TTGAGCTCGG ACCCTGGCAT GCATGTGAGA
    CAGGCAAAGG AGGCACCTGC CAAGCTGGAG TCGCAGGCTG GGCAGCAGGT GTGCCGCGGC
    GCCCGGGACC GGGTCCGCTC CATGTCCGGG GGCCACGGTC TTCGTGTTGG CGCCCTGGAC
    ACTCCAGCCT CCCACTTGCC GGGTGGCCCC ACTTCCCCAG GCACGCAGGC AGCGCTGGCC
    AGCAAGTCTG ACAGGGCCTC GGCTGGCACC CGGTTCCCAG CACAGGAGAA GACGAGCCTG
    GCAGCCTACG TACCGCTGCT GACCCAGGGC TGGGCGGAGA TCTTGGTCCG GAGGCCTACA
    GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCCTTCTC GTCGGACATC
    AACAACATGC CTCTGCAGGA GCTGTCCAAC GCGCTCATGG CCGCTGAGCG CTTCAAGGAG
    CGTCGTGACA CGGCTCTGTA CAAGTCGCTG TCGGTGCCTG CCGCAGGCTC GGCCAAGCCC
    CCTCCGCCCC CGAGATCTAA CACGGTGGCC TCTTTCTCCT CCCTGTACCA GTCCAGTTGC
    CAAGGAAAGC TGCACAGGAG CATTTCCTGG GCAGACTCTG CGGTGGTTCT GGAGGAGGGA
    AATTCAAGCG AGACCAGTCT GCCAGTGGAG CACACGGAGC TGGAGGACTT CGAGGCGACA
    CTAGGCACAG ACCGGCGCTG TGGCCGCGCG GAAGCTTACA GCAGGTCGTC TTCAAGCTCC
    AGCCAGGAGG AGAAGTTCCC TGCAGAGGAG CTGGCTGCCG GAGGGATCCC CATCGAACGA
    GCTGTCTCTT CCGAGGGTGC CCGGCCTTCT GTGGAGCTCT CCTTCCAGCC CTCCCAGCCC
    CTCAGCAAGT CCAGCTCCTC CCCTGAGCTG CAGACCCTGC AGGACATACT CGGGGACCCC
    GGGGACAAGT CTGATGTTGG CCGGCTCAGC CCTGAGGCCA AGGCCCGGTC ACAGTCCGGG
    ATTCTGGACG GGGTGGGTGC CTCCTGGTCA GCCCCAGGTG AAGAGAACCA GGGCCAGGGC
    CCCGCACAGC CCGAGGGTCC CTTGCCTTCC AGCTGCCCCC GCTCCCCCAG TGGCCTGCGG
    CCCAGAGGCT ACACCATCTC TGATTCAGCC CCGTCACGCA GAGGCAAGAG GGTAGAGCGG
    GACGCCTTCA GGAGCAGAGC TGGGGCTTCC AACACTGAGA AGGTGCCAGG CATCAATCCC
    AGCTTTGTGT TCCTGCAGCT CTACCACTCA CCCTTCTTTG GTGACGAGTC CAACAAGCCC
    ATCCTTCTAC CCAATGAGTC CTTCGAGCGG TCAGTGCAGC TTCTCGACCA GATTCCATCA
    TACGACACAC ACAAGATTGC CGTCCTGTAC GTGGGAGAAG GCCAGAGCAG CAGTGAGCTC
    GCCATCCTGT CCAATGAGCA TGGCTCGTAC AGGTACACGG AGTTCCTGAC AGGCCTGGGC
    AAGCTCATTG AGCTCAAAGA CTGCCAGCCG GACAAGGTGT ACCTGGGGGG CCTGGACGTG
    TGTGGCGAAG ATGGCCAGTT CACCTACTGC TGGCATGACG ACATCATGCA AGCCGTCTTC
    CACATTGCTA CCCTGATGCC CACCAAGGAA GTGGACAAGC ACCGCTGTGA CAAGAAGCGC
    CACCTGGGCA ATGACTTTGT GTCCATCGTC TACAACGACT CTGGTGAGGA CTTCAAGCTG
    GGCACCATCC GAGGCCAGTT CAACTTCGTC CACGTGATCA TCACCCCCCT GGACTATGAG
    TGCAACCTGG TGTCCCTGCA GTGCAGGAAA GACATGGAAG GTCTTGTGGA TACTAGCGTG
    GCCAAGATCG TGTCTGACCG CAACTTGCCT TTTGTGGCAC GTCAGATGGC TTTGCATGCA
    AATATGGCCT CACAAGTGCA TCATAGCCGC TCCAACCCCA CCGACATCTA TCCCTCCAAG
    TGGATTGCCA GGCTTCGCCA CATCAAGCGA CTCCGCCACC GGATCCGCGA GGAAGCCCAC
    TACACCAACC CCAGCCTGCC ACTGATGCAG ATGCACCCCC CGGGCCACCC CAAAGCCTCT
    GCTCAGGCGC CGGCAGAGCC TGTGCCCACG TATGAGACAG GCCAGCGGAA GCGTCTCATC
    TCCTCTGTGG ATGACTTCAC GGAGTTTGTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe08333
    Clone ID Related Accession (Same CDS sequence) XM_005621718.2 , XM_005621718.3
    Accession Version XM_005621718.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5313bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product tuberin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005621718.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
    CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC
    ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG
    ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG
    CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA
    GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG
    GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG
    GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG
    GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT
    GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC
    ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC
    GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG
    CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG
    GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG
    ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG
    CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC
    TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC
    CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC
    AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC
    CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG
    TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC
    TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT
    GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA
    GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT
    CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG
    AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC
    AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT
    AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG
    CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC
    AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT
    GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG
    CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG
    GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG
    GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG
    CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC
    GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG
    GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC
    GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC
    ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC
    GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG
    TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC
    CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC
    CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC
    AACGAGAGGC CCAAGAGCAG GATCCAGACG TCCCTCACAA GCGCCAGCTT GGGGTCTGCT
    GACGAGAACT CGATGGCCCA GGCTGATGAC AACTTGAAAA ATCTCCACCT GGAGCTCACG
    GAAACATGTC TGGACATGAT GGCCAGATAT GTGTTCTCCA ACTTCACAGC AGTCCCCAAG
    AGGTCCCCGG TGGGAGAGTT CCTCCTGGCT GGTGGCAGGA CCAAAACCTG GCTGGTTGGG
    AACAAGCTTG TCACTGTGAC GACGAGCGTG GGGACGGGGA CCCGGTCGCT GCTGGGCTTG
    GACTCAGGAG ATCAGCAAGG TGGCCCCGAG TTGAGCTCGG ACCCTGGCAT GCATGTGAGA
    CAGGCAAAGG AGGCACCTGC CAAGCTGGAG TCGCAGGCTG GGCAGCAGGT GTGCCGCGGC
    GCCCGGGACC GGGTCCGCTC CATGTCCGGG GGCCACGGTC TTCGTGTTGG CGCCCTGGAC
    ACTCCAGCCT CCCACTTGCC GGGTGGCCCC ACTTCCCCAG GCACGCAGGC AGCGCTGGCC
    AGCAAGTCTG ACAGGGCCTC GGCTGGCACC CGGTTCCCAG CACAGGAGAA GACGAGCCTG
    GCAGCCTACG TACCGCTGCT GACCCAGGGC TGGGCGGAGA TCTTGGTCCG GAGGCCTACA
    GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCCTTCTC GTCGGACATC
    AACAACATGC CTCTGCAGGA GCTGTCCAAC GCGCTCATGG CCGCTGAGCG CTTCAAGGAG
    CGTCGTGACA CGGCTCTGTA CAAGTCGCTG TCGGTGCCTG CCGCAGGCTC GGCCAAGCCC
    CCTCCGCCCC CGAGATCTAA CACGGTGGCC TCTTTCTCCT CCCTGTACCA GTCCAGTTGC
    CAAGGAAAGC TGCACAGGAG CATTTCCTGG GCAGACTCTG CGGTGGTTCT GGAGGAGGGA
    AATTCAAGCG AGACCAGTCT GCCAGTGGAG CACACGGAGC TGGAGGACTT CGAGGCGACA
    CTAGGCACAG ACCGGCGCTG TGGCCGCGCG GAAGCTTACA GCAGGTCGTC TTCAAGCTCC
    AGCCAGGAGG AGAAGTTCCC TGCAGAGGAG CTGGCTGCCG GAGGGATCCC CATCGAACGA
    GCTGTCTCTT CCGAGGGTGC CCGGCCTTCT GTGGAGCTCT CCTTCCAGCC CTCCCAGCCC
    CTCAGCAAGT CCAGCTCCTC CCCTGAGCTG CAGACCCTGC AGGACATACT CGGGGACCCC
    GGGGACAAGT CTGATGTTGG CCGGCTCAGC CCTGAGGCCA AGGCCCGGTC ACAGTCCGGG
    ATTCTGGACG GGGTGGGTGC CTCCTGGTCA GCCCCAGGTG AAGAGAACCA GGGCCAGGGC
    CCCGCACAGC CCGAGGGTCC CTTGCCTTCC AGCTGCCCCC GCTCCCCCAG TGGCCTGCGG
    CCCAGAGGCT ACACCATCTC TGATTCAGCC CCGTCACGCA GAGGCAAGAG GGTAGAGCGG
    GACGCCTTCA GGAGCAGAGC TGGGGCTTCC AACACTGAGA AGGTGCCAGG CATCAATCCC
    AGCTTTGTGT TCCTGCAGCT CTACCACTCA CCCTTCTTTG GTGACGAGTC CAACAAGCCC
    ATCCTTCTAC CCAATGAGTC CTTCGAGCGG TCAGTGCAGC TTCTCGACCA GATTCCATCA
    TACGACACAC ACAAGATTGC CGTCCTGTAC GTGGGAGAAG GCCAGAGCAG CAGTGAGCTC
    GCCATCCTGT CCAATGAGCA TGGCTCGTAC AGGTACACGG AGTTCCTGAC AGGCCTGGGC
    AAGCTCATTG AGCTCAAAGA CTGCCAGCCG GACAAGGTGT ACCTGGGGGG CCTGGACGTG
    TGTGGCGAAG ATGGCCAGTT CACCTACTGC TGGCATGACG ACATCATGCA AGCCGTCTTC
    CACATTGCTA CCCTGATGCC CACCAAGGAA GTGGACAAGC ACCGCTGTGA CAAGAAGCGC
    CACCTGGGCA ATGACTTTGT GTCCATCGTC TACAACGACT CTGGTGAGGA CTTCAAGCTG
    GGCACCATCC GAGGCCAGTT CAACTTCGTC CACGTGATCA TCACCCCCCT GGACTATGAG
    TGCAACCTGG TGTCCCTGCA GTGCAGGAAA GACATGGAAG GTCTTGTGGA TACTAGCGTG
    GCCAAGATCG TGTCTGACCG CAACTTGCCT TTTGTGGCAC GTCAGATGGC TTTGCATGCA
    AATATGGCCT CACAAGTGCA TCATAGCCGC TCCAACCCCA CCGACATCTA TCCCTCCAAG
    TGGATTGCCA GGCTTCGCCA CATCAAGCGA CTCCGCCACC GGATCCGCGA GGAAGCCCAC
    TACACCAACC CCAGCCTGCC ACTGATGCAG ATGCACCCCC CGGGCCACCC CAAAGCCTCT
    GCTCAGGCGC CGGCAGAGCC TGTGCCCACG TATGAGACAG GCCAGCGGAA GCGTCTCATC
    TCCTCTGTGG ATGACTTCAC GGAGTTTGTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005621775.1
    CDS290..5602
    Translation

    Target ORF information:

    RefSeq Version XM_005621718.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005621718.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
    CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC
    ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG
    ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG
    CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA
    GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG
    GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG
    GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG
    GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT
    GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC
    ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC
    GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG
    CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG
    GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG
    ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG
    CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC
    TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC
    CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC
    AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC
    CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG
    TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC
    TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT
    GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA
    GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT
    CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG
    AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC
    AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT
    AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG
    CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC
    AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT
    GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG
    CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG
    GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG
    GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG
    CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC
    GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG
    GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC
    GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC
    ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC
    GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG
    TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC
    CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC
    CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC
    AACGAGAGGC CCAAGAGCAG GATCCAGACG TCCCTCACAA GCGCCAGCTT GGGGTCTGCT
    GACGAGAACT CGATGGCCCA GGCTGATGAC AACTTGAAAA ATCTCCACCT GGAGCTCACG
    GAAACATGTC TGGACATGAT GGCCAGATAT GTGTTCTCCA ACTTCACAGC AGTCCCCAAG
    AGGTCCCCGG TGGGAGAGTT CCTCCTGGCT GGTGGCAGGA CCAAAACCTG GCTGGTTGGG
    AACAAGCTTG TCACTGTGAC GACGAGCGTG GGGACGGGGA CCCGGTCGCT GCTGGGCTTG
    GACTCAGGAG ATCAGCAAGG TGGCCCCGAG TTGAGCTCGG ACCCTGGCAT GCATGTGAGA
    CAGGCAAAGG AGGCACCTGC CAAGCTGGAG TCGCAGGCTG GGCAGCAGGT GTGCCGCGGC
    GCCCGGGACC GGGTCCGCTC CATGTCCGGG GGCCACGGTC TTCGTGTTGG CGCCCTGGAC
    ACTCCAGCCT CCCACTTGCC GGGTGGCCCC ACTTCCCCAG GCACGCAGGC AGCGCTGGCC
    AGCAAGTCTG ACAGGGCCTC GGCTGGCACC CGGTTCCCAG CACAGGAGAA GACGAGCCTG
    GCAGCCTACG TACCGCTGCT GACCCAGGGC TGGGCGGAGA TCTTGGTCCG GAGGCCTACA
    GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCCTTCTC GTCGGACATC
    AACAACATGC CTCTGCAGGA GCTGTCCAAC GCGCTCATGG CCGCTGAGCG CTTCAAGGAG
    CGTCGTGACA CGGCTCTGTA CAAGTCGCTG TCGGTGCCTG CCGCAGGCTC GGCCAAGCCC
    CCTCCGCCCC CGAGATCTAA CACGGTGGCC TCTTTCTCCT CCCTGTACCA GTCCAGTTGC
    CAAGGAAAGC TGCACAGGAG CATTTCCTGG GCAGACTCTG CGGTGGTTCT GGAGGAGGGA
    AATTCAAGCG AGACCAGTCT GCCAGTGGAG CACACGGAGC TGGAGGACTT CGAGGCGACA
    CTAGGCACAG ACCGGCGCTG TGGCCGCGCG GAAGCTTACA GCAGGTCGTC TTCAAGCTCC
    AGCCAGGAGG AGAAGTTCCC TGCAGAGGAG CTGGCTGCCG GAGGGATCCC CATCGAACGA
    GCTGTCTCTT CCGAGGGTGC CCGGCCTTCT GTGGAGCTCT CCTTCCAGCC CTCCCAGCCC
    CTCAGCAAGT CCAGCTCCTC CCCTGAGCTG CAGACCCTGC AGGACATACT CGGGGACCCC
    GGGGACAAGT CTGATGTTGG CCGGCTCAGC CCTGAGGCCA AGGCCCGGTC ACAGTCCGGG
    ATTCTGGACG GGGTGGGTGC CTCCTGGTCA GCCCCAGGTG AAGAGAACCA GGGCCAGGGC
    CCCGCACAGC CCGAGGGTCC CTTGCCTTCC AGCTGCCCCC GCTCCCCCAG TGGCCTGCGG
    CCCAGAGGCT ACACCATCTC TGATTCAGCC CCGTCACGCA GAGGCAAGAG GGTAGAGCGG
    GACGCCTTCA GGAGCAGAGC TGGGGCTTCC AACACTGAGA AGGTGCCAGG CATCAATCCC
    AGCTTTGTGT TCCTGCAGCT CTACCACTCA CCCTTCTTTG GTGACGAGTC CAACAAGCCC
    ATCCTTCTAC CCAATGAGTC CTTCGAGCGG TCAGTGCAGC TTCTCGACCA GATTCCATCA
    TACGACACAC ACAAGATTGC CGTCCTGTAC GTGGGAGAAG GCCAGAGCAG CAGTGAGCTC
    GCCATCCTGT CCAATGAGCA TGGCTCGTAC AGGTACACGG AGTTCCTGAC AGGCCTGGGC
    AAGCTCATTG AGCTCAAAGA CTGCCAGCCG GACAAGGTGT ACCTGGGGGG CCTGGACGTG
    TGTGGCGAAG ATGGCCAGTT CACCTACTGC TGGCATGACG ACATCATGCA AGCCGTCTTC
    CACATTGCTA CCCTGATGCC CACCAAGGAA GTGGACAAGC ACCGCTGTGA CAAGAAGCGC
    CACCTGGGCA ATGACTTTGT GTCCATCGTC TACAACGACT CTGGTGAGGA CTTCAAGCTG
    GGCACCATCC GAGGCCAGTT CAACTTCGTC CACGTGATCA TCACCCCCCT GGACTATGAG
    TGCAACCTGG TGTCCCTGCA GTGCAGGAAA GACATGGAAG GTCTTGTGGA TACTAGCGTG
    GCCAAGATCG TGTCTGACCG CAACTTGCCT TTTGTGGCAC GTCAGATGGC TTTGCATGCA
    AATATGGCCT CACAAGTGCA TCATAGCCGC TCCAACCCCA CCGACATCTA TCCCTCCAAG
    TGGATTGCCA GGCTTCGCCA CATCAAGCGA CTCCGCCACC GGATCCGCGA GGAAGCCCAC
    TACACCAACC CCAGCCTGCC ACTGATGCAG ATGCACCCCC CGGGCCACCC CAAAGCCTCT
    GCTCAGGCGC CGGCAGAGCC TGTGCCCACG TATGAGACAG GCCAGCGGAA GCGTCTCATC
    TCCTCTGTGG ATGACTTCAC GGAGTTTGTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe08334
    Clone ID Related Accession (Same CDS sequence) XM_005621719.3 , XM_005621719.2
    Accession Version XM_005621719.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5310bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product tuberin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005621719.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
    CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC
    ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG
    ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG
    CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA
    GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG
    GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG
    GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG
    GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT
    GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC
    ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC
    GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG
    CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG
    GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG
    ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG
    CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC
    TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC
    CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC
    AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC
    CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG
    TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC
    TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT
    GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA
    GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT
    CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG
    AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC
    AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT
    AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG
    CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC
    AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT
    GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG
    CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG
    GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG
    GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG
    CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC
    GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG
    GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC
    GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC
    ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC
    GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG
    TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC
    CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC
    CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC
    AACGAGAGGC CCAAGAGGAT CCAGACGTCC CTCACAAGCG CCAGCTTGGG GTCTGCTGAC
    GAGAACTCGA TGGCCCAGGC TGATGACAAC TTGAAAAATC TCCACCTGGA GCTCACGGAA
    ACATGTCTGG ACATGATGGC CAGATATGTG TTCTCCAACT TCACAGCAGT CCCCAAGAGG
    TCCCCGGTGG GAGAGTTCCT CCTGGCTGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC
    AAGCTTGTCA CTGTGACGAC GAGCGTGGGG ACGGGGACCC GGTCGCTGCT GGGCTTGGAC
    TCAGGAGATC AGCAAGGTGG CCCCGAGTTG AGCTCGGACC CTGGCATGCA TGTGAGACAG
    GCAAAGGAGG CACCTGCCAA GCTGGAGTCG CAGGCTGGGC AGCAGGTGTG CCGCGGCGCC
    CGGGACCGGG TCCGCTCCAT GTCCGGGGGC CACGGTCTTC GTGTTGGCGC CCTGGACACT
    CCAGCCTCCC ACTTGCCGGG TGGCCCCACT TCCCCAGGCA CGCAGGCAGC GCTGGCCAGC
    AAGTCTGACA GGGCCTCGGC TGGCACCCGG TTCCCAGCAC AGGAGAAGAC GAGCCTGGCA
    GCCTACGTAC CGCTGCTGAC CCAGGGCTGG GCGGAGATCT TGGTCCGGAG GCCTACAGGG
    AACACCAGCT GGCTGATGAG CCTGGAGAAC CCGCTCAGCC CCTTCTCGTC GGACATCAAC
    AACATGCCTC TGCAGGAGCT GTCCAACGCG CTCATGGCCG CTGAGCGCTT CAAGGAGCGT
    CGTGACACGG CTCTGTACAA GTCGCTGTCG GTGCCTGCCG CAGGCTCGGC CAAGCCCCCT
    CCGCCCCCGA GATCTAACAC GGTGGCCTCT TTCTCCTCCC TGTACCAGTC CAGTTGCCAA
    GGAAAGCTGC ACAGGAGCAT TTCCTGGGCA GACTCTGCGG TGGTTCTGGA GGAGGGAAAT
    TCAAGCGAGA CCAGTCTGCC AGTGGAGCAC ACGGAGCTGG AGGACTTCGA GGCGACACTA
    GGCACAGACC GGCGCTGTGG CCGCGCGGAA GCTTACAGCA GGTCGTCTTC AAGCTCCAGC
    CAGGAGGAGA AGTTCCCTGC AGAGGAGCTG GCTGCCGGAG GGATCCCCAT CGAACGAGCT
    GTCTCTTCCG AGGGTGCCCG GCCTTCTGTG GAGCTCTCCT TCCAGCCCTC CCAGCCCCTC
    AGCAAGTCCA GCTCCTCCCC TGAGCTGCAG ACCCTGCAGG ACATACTCGG GGACCCCGGG
    GACAAGTCTG ATGTTGGCCG GCTCAGCCCT GAGGCCAAGG CCCGGTCACA GTCCGGGATT
    CTGGACGGGG TGGGTGCCTC CTGGTCAGCC CCAGGTGAAG AGAACCAGGG CCAGGGCCCC
    GCACAGCCCG AGGGTCCCTT GCCTTCCAGC TGCCCCCGCT CCCCCAGTGG CCTGCGGCCC
    AGAGGCTACA CCATCTCTGA TTCAGCCCCG TCACGCAGAG GCAAGAGGGT AGAGCGGGAC
    GCCTTCAGGA GCAGAGCTGG GGCTTCCAAC ACTGAGAAGG TGCCAGGCAT CAATCCCAGC
    TTTGTGTTCC TGCAGCTCTA CCACTCACCC TTCTTTGGTG ACGAGTCCAA CAAGCCCATC
    CTTCTACCCA ATGAGTCCTT CGAGCGGTCA GTGCAGCTTC TCGACCAGAT TCCATCATAC
    GACACACACA AGATTGCCGT CCTGTACGTG GGAGAAGGCC AGAGCAGCAG TGAGCTCGCC
    ATCCTGTCCA ATGAGCATGG CTCGTACAGG TACACGGAGT TCCTGACAGG CCTGGGCAAG
    CTCATTGAGC TCAAAGACTG CCAGCCGGAC AAGGTGTACC TGGGGGGCCT GGACGTGTGT
    GGCGAAGATG GCCAGTTCAC CTACTGCTGG CATGACGACA TCATGCAAGC CGTCTTCCAC
    ATTGCTACCC TGATGCCCAC CAAGGAAGTG GACAAGCACC GCTGTGACAA GAAGCGCCAC
    CTGGGCAATG ACTTTGTGTC CATCGTCTAC AACGACTCTG GTGAGGACTT CAAGCTGGGC
    ACCATCCGAG GCCAGTTCAA CTTCGTCCAC GTGATCATCA CCCCCCTGGA CTATGAGTGC
    AACCTGGTGT CCCTGCAGTG CAGGAAAGAC ATGGAAGGTC TTGTGGATAC TAGCGTGGCC
    AAGATCGTGT CTGACCGCAA CTTGCCTTTT GTGGCACGTC AGATGGCTTT GCATGCAAAT
    ATGGCCTCAC AAGTGCATCA TAGCCGCTCC AACCCCACCG ACATCTATCC CTCCAAGTGG
    ATTGCCAGGC TTCGCCACAT CAAGCGACTC CGCCACCGGA TCCGCGAGGA AGCCCACTAC
    ACCAACCCCA GCCTGCCACT GATGCAGATG CACCCCCCGG GCCACCCCAA AGCCTCTGCT
    CAGGCGCCGG CAGAGCCTGT GCCCACGTAT GAGACAGGCC AGCGGAAGCG TCTCATCTCC
    TCTGTGGATG ACTTCACGGA GTTTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005621776.1
    CDS289..5598
    Translation

    Target ORF information:

    RefSeq Version XM_005621719.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005621719.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
    CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC
    ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG
    ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG
    CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA
    GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG
    GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG
    GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG
    GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT
    GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC
    ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC
    GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG
    CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG
    GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG
    ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG
    CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC
    TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC
    CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC
    AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC
    CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG
    TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC
    TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT
    GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA
    GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT
    CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG
    AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC
    AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT
    AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG
    CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC
    AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT
    GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG
    CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG
    GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG
    GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG
    CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC
    GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG
    GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC
    GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC
    ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC
    GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG
    TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC
    CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC
    CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC
    AACGAGAGGC CCAAGAGGAT CCAGACGTCC CTCACAAGCG CCAGCTTGGG GTCTGCTGAC
    GAGAACTCGA TGGCCCAGGC TGATGACAAC TTGAAAAATC TCCACCTGGA GCTCACGGAA
    ACATGTCTGG ACATGATGGC CAGATATGTG TTCTCCAACT TCACAGCAGT CCCCAAGAGG
    TCCCCGGTGG GAGAGTTCCT CCTGGCTGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC
    AAGCTTGTCA CTGTGACGAC GAGCGTGGGG ACGGGGACCC GGTCGCTGCT GGGCTTGGAC
    TCAGGAGATC AGCAAGGTGG CCCCGAGTTG AGCTCGGACC CTGGCATGCA TGTGAGACAG
    GCAAAGGAGG CACCTGCCAA GCTGGAGTCG CAGGCTGGGC AGCAGGTGTG CCGCGGCGCC
    CGGGACCGGG TCCGCTCCAT GTCCGGGGGC CACGGTCTTC GTGTTGGCGC CCTGGACACT
    CCAGCCTCCC ACTTGCCGGG TGGCCCCACT TCCCCAGGCA CGCAGGCAGC GCTGGCCAGC
    AAGTCTGACA GGGCCTCGGC TGGCACCCGG TTCCCAGCAC AGGAGAAGAC GAGCCTGGCA
    GCCTACGTAC CGCTGCTGAC CCAGGGCTGG GCGGAGATCT TGGTCCGGAG GCCTACAGGG
    AACACCAGCT GGCTGATGAG CCTGGAGAAC CCGCTCAGCC CCTTCTCGTC GGACATCAAC
    AACATGCCTC TGCAGGAGCT GTCCAACGCG CTCATGGCCG CTGAGCGCTT CAAGGAGCGT
    CGTGACACGG CTCTGTACAA GTCGCTGTCG GTGCCTGCCG CAGGCTCGGC CAAGCCCCCT
    CCGCCCCCGA GATCTAACAC GGTGGCCTCT TTCTCCTCCC TGTACCAGTC CAGTTGCCAA
    GGAAAGCTGC ACAGGAGCAT TTCCTGGGCA GACTCTGCGG TGGTTCTGGA GGAGGGAAAT
    TCAAGCGAGA CCAGTCTGCC AGTGGAGCAC ACGGAGCTGG AGGACTTCGA GGCGACACTA
    GGCACAGACC GGCGCTGTGG CCGCGCGGAA GCTTACAGCA GGTCGTCTTC AAGCTCCAGC
    CAGGAGGAGA AGTTCCCTGC AGAGGAGCTG GCTGCCGGAG GGATCCCCAT CGAACGAGCT
    GTCTCTTCCG AGGGTGCCCG GCCTTCTGTG GAGCTCTCCT TCCAGCCCTC CCAGCCCCTC
    AGCAAGTCCA GCTCCTCCCC TGAGCTGCAG ACCCTGCAGG ACATACTCGG GGACCCCGGG
    GACAAGTCTG ATGTTGGCCG GCTCAGCCCT GAGGCCAAGG CCCGGTCACA GTCCGGGATT
    CTGGACGGGG TGGGTGCCTC CTGGTCAGCC CCAGGTGAAG AGAACCAGGG CCAGGGCCCC
    GCACAGCCCG AGGGTCCCTT GCCTTCCAGC TGCCCCCGCT CCCCCAGTGG CCTGCGGCCC
    AGAGGCTACA CCATCTCTGA TTCAGCCCCG TCACGCAGAG GCAAGAGGGT AGAGCGGGAC
    GCCTTCAGGA GCAGAGCTGG GGCTTCCAAC ACTGAGAAGG TGCCAGGCAT CAATCCCAGC
    TTTGTGTTCC TGCAGCTCTA CCACTCACCC TTCTTTGGTG ACGAGTCCAA CAAGCCCATC
    CTTCTACCCA ATGAGTCCTT CGAGCGGTCA GTGCAGCTTC TCGACCAGAT TCCATCATAC
    GACACACACA AGATTGCCGT CCTGTACGTG GGAGAAGGCC AGAGCAGCAG TGAGCTCGCC
    ATCCTGTCCA ATGAGCATGG CTCGTACAGG TACACGGAGT TCCTGACAGG CCTGGGCAAG
    CTCATTGAGC TCAAAGACTG CCAGCCGGAC AAGGTGTACC TGGGGGGCCT GGACGTGTGT
    GGCGAAGATG GCCAGTTCAC CTACTGCTGG CATGACGACA TCATGCAAGC CGTCTTCCAC
    ATTGCTACCC TGATGCCCAC CAAGGAAGTG GACAAGCACC GCTGTGACAA GAAGCGCCAC
    CTGGGCAATG ACTTTGTGTC CATCGTCTAC AACGACTCTG GTGAGGACTT CAAGCTGGGC
    ACCATCCGAG GCCAGTTCAA CTTCGTCCAC GTGATCATCA CCCCCCTGGA CTATGAGTGC
    AACCTGGTGT CCCTGCAGTG CAGGAAAGAC ATGGAAGGTC TTGTGGATAC TAGCGTGGCC
    AAGATCGTGT CTGACCGCAA CTTGCCTTTT GTGGCACGTC AGATGGCTTT GCATGCAAAT
    ATGGCCTCAC AAGTGCATCA TAGCCGCTCC AACCCCACCG ACATCTATCC CTCCAAGTGG
    ATTGCCAGGC TTCGCCACAT CAAGCGACTC CGCCACCGGA TCCGCGAGGA AGCCCACTAC
    ACCAACCCCA GCCTGCCACT GATGCAGATG CACCCCCCGG GCCACCCCAA AGCCTCTGCT
    CAGGCGCCGG CAGAGCCTGT GCCCACGTAT GAGACAGGCC AGCGGAAGCG TCTCATCTCC
    TCTGTGGATG ACTTCACGGA GTTTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe08334
    Clone ID Related Accession (Same CDS sequence) XM_005621719.3 , XM_005621719.2
    Accession Version XM_005621719.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5310bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product tuberin isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005621719.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
    CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC
    ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG
    ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG
    CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA
    GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG
    GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG
    GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG
    GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT
    GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC
    ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC
    GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG
    CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG
    GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG
    ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG
    CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC
    TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC
    CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC
    AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC
    CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG
    TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC
    TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT
    GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA
    GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT
    CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG
    AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC
    AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT
    AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG
    CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC
    AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT
    GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG
    CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG
    GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG
    GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG
    CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC
    GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG
    GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC
    GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC
    ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC
    GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG
    TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC
    CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC
    CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC
    AACGAGAGGC CCAAGAGGAT CCAGACGTCC CTCACAAGCG CCAGCTTGGG GTCTGCTGAC
    GAGAACTCGA TGGCCCAGGC TGATGACAAC TTGAAAAATC TCCACCTGGA GCTCACGGAA
    ACATGTCTGG ACATGATGGC CAGATATGTG TTCTCCAACT TCACAGCAGT CCCCAAGAGG
    TCCCCGGTGG GAGAGTTCCT CCTGGCTGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC
    AAGCTTGTCA CTGTGACGAC GAGCGTGGGG ACGGGGACCC GGTCGCTGCT GGGCTTGGAC
    TCAGGAGATC AGCAAGGTGG CCCCGAGTTG AGCTCGGACC CTGGCATGCA TGTGAGACAG
    GCAAAGGAGG CACCTGCCAA GCTGGAGTCG CAGGCTGGGC AGCAGGTGTG CCGCGGCGCC
    CGGGACCGGG TCCGCTCCAT GTCCGGGGGC CACGGTCTTC GTGTTGGCGC CCTGGACACT
    CCAGCCTCCC ACTTGCCGGG TGGCCCCACT TCCCCAGGCA CGCAGGCAGC GCTGGCCAGC
    AAGTCTGACA GGGCCTCGGC TGGCACCCGG TTCCCAGCAC AGGAGAAGAC GAGCCTGGCA
    GCCTACGTAC CGCTGCTGAC CCAGGGCTGG GCGGAGATCT TGGTCCGGAG GCCTACAGGG
    AACACCAGCT GGCTGATGAG CCTGGAGAAC CCGCTCAGCC CCTTCTCGTC GGACATCAAC
    AACATGCCTC TGCAGGAGCT GTCCAACGCG CTCATGGCCG CTGAGCGCTT CAAGGAGCGT
    CGTGACACGG CTCTGTACAA GTCGCTGTCG GTGCCTGCCG CAGGCTCGGC CAAGCCCCCT
    CCGCCCCCGA GATCTAACAC GGTGGCCTCT TTCTCCTCCC TGTACCAGTC CAGTTGCCAA
    GGAAAGCTGC ACAGGAGCAT TTCCTGGGCA GACTCTGCGG TGGTTCTGGA GGAGGGAAAT
    TCAAGCGAGA CCAGTCTGCC AGTGGAGCAC ACGGAGCTGG AGGACTTCGA GGCGACACTA
    GGCACAGACC GGCGCTGTGG CCGCGCGGAA GCTTACAGCA GGTCGTCTTC AAGCTCCAGC
    CAGGAGGAGA AGTTCCCTGC AGAGGAGCTG GCTGCCGGAG GGATCCCCAT CGAACGAGCT
    GTCTCTTCCG AGGGTGCCCG GCCTTCTGTG GAGCTCTCCT TCCAGCCCTC CCAGCCCCTC
    AGCAAGTCCA GCTCCTCCCC TGAGCTGCAG ACCCTGCAGG ACATACTCGG GGACCCCGGG
    GACAAGTCTG ATGTTGGCCG GCTCAGCCCT GAGGCCAAGG CCCGGTCACA GTCCGGGATT
    CTGGACGGGG TGGGTGCCTC CTGGTCAGCC CCAGGTGAAG AGAACCAGGG CCAGGGCCCC
    GCACAGCCCG AGGGTCCCTT GCCTTCCAGC TGCCCCCGCT CCCCCAGTGG CCTGCGGCCC
    AGAGGCTACA CCATCTCTGA TTCAGCCCCG TCACGCAGAG GCAAGAGGGT AGAGCGGGAC
    GCCTTCAGGA GCAGAGCTGG GGCTTCCAAC ACTGAGAAGG TGCCAGGCAT CAATCCCAGC
    TTTGTGTTCC TGCAGCTCTA CCACTCACCC TTCTTTGGTG ACGAGTCCAA CAAGCCCATC
    CTTCTACCCA ATGAGTCCTT CGAGCGGTCA GTGCAGCTTC TCGACCAGAT TCCATCATAC
    GACACACACA AGATTGCCGT CCTGTACGTG GGAGAAGGCC AGAGCAGCAG TGAGCTCGCC
    ATCCTGTCCA ATGAGCATGG CTCGTACAGG TACACGGAGT TCCTGACAGG CCTGGGCAAG
    CTCATTGAGC TCAAAGACTG CCAGCCGGAC AAGGTGTACC TGGGGGGCCT GGACGTGTGT
    GGCGAAGATG GCCAGTTCAC CTACTGCTGG CATGACGACA TCATGCAAGC CGTCTTCCAC
    ATTGCTACCC TGATGCCCAC CAAGGAAGTG GACAAGCACC GCTGTGACAA GAAGCGCCAC
    CTGGGCAATG ACTTTGTGTC CATCGTCTAC AACGACTCTG GTGAGGACTT CAAGCTGGGC
    ACCATCCGAG GCCAGTTCAA CTTCGTCCAC GTGATCATCA CCCCCCTGGA CTATGAGTGC
    AACCTGGTGT CCCTGCAGTG CAGGAAAGAC ATGGAAGGTC TTGTGGATAC TAGCGTGGCC
    AAGATCGTGT CTGACCGCAA CTTGCCTTTT GTGGCACGTC AGATGGCTTT GCATGCAAAT
    ATGGCCTCAC AAGTGCATCA TAGCCGCTCC AACCCCACCG ACATCTATCC CTCCAAGTGG
    ATTGCCAGGC TTCGCCACAT CAAGCGACTC CGCCACCGGA TCCGCGAGGA AGCCCACTAC
    ACCAACCCCA GCCTGCCACT GATGCAGATG CACCCCCCGG GCCACCCCAA AGCCTCTGCT
    CAGGCGCCGG CAGAGCCTGT GCCCACGTAT GAGACAGGCC AGCGGAAGCG TCTCATCTCC
    TCTGTGGATG ACTTCACGGA GTTTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005621776.1
    CDS283..5592
    Translation

    Target ORF information:

    RefSeq Version XM_005621719.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris tuberous sclerosis 2 (TSC2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005621719.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
    CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC
    ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG
    ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG
    CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA
    GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG
    GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG
    GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG
    GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT
    GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC
    ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC
    GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG
    CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG
    GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG
    ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG
    CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC
    TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC
    CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC
    AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC
    CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG
    TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC
    TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT
    GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA
    GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT
    CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG
    AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC
    AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT
    AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG
    CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC
    AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT
    GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG
    CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG
    GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG
    GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG
    CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC
    GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG
    GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC
    GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC
    ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC
    GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG
    TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC
    CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC
    CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC
    AACGAGAGGC CCAAGAGGAT CCAGACGTCC CTCACAAGCG CCAGCTTGGG GTCTGCTGAC
    GAGAACTCGA TGGCCCAGGC TGATGACAAC TTGAAAAATC TCCACCTGGA GCTCACGGAA
    ACATGTCTGG ACATGATGGC CAGATATGTG TTCTCCAACT TCACAGCAGT CCCCAAGAGG
    TCCCCGGTGG GAGAGTTCCT CCTGGCTGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC
    AAGCTTGTCA CTGTGACGAC GAGCGTGGGG ACGGGGACCC GGTCGCTGCT GGGCTTGGAC
    TCAGGAGATC AGCAAGGTGG CCCCGAGTTG AGCTCGGACC CTGGCATGCA TGTGAGACAG
    GCAAAGGAGG CACCTGCCAA GCTGGAGTCG CAGGCTGGGC AGCAGGTGTG CCGCGGCGCC
    CGGGACCGGG TCCGCTCCAT GTCCGGGGGC CACGGTCTTC GTGTTGGCGC CCTGGACACT
    CCAGCCTCCC ACTTGCCGGG TGGCCCCACT TCCCCAGGCA CGCAGGCAGC GCTGGCCAGC
    AAGTCTGACA GGGCCTCGGC TGGCACCCGG TTCCCAGCAC AGGAGAAGAC GAGCCTGGCA
    GCCTACGTAC CGCTGCTGAC CCAGGGCTGG GCGGAGATCT TGGTCCGGAG GCCTACAGGG
    AACACCAGCT GGCTGATGAG CCTGGAGAAC CCGCTCAGCC CCTTCTCGTC GGACATCAAC
    AACATGCCTC TGCAGGAGCT GTCCAACGCG CTCATGGCCG CTGAGCGCTT CAAGGAGCGT
    CGTGACACGG CTCTGTACAA GTCGCTGTCG GTGCCTGCCG CAGGCTCGGC CAAGCCCCCT
    CCGCCCCCGA GATCTAACAC GGTGGCCTCT TTCTCCTCCC TGTACCAGTC CAGTTGCCAA
    GGAAAGCTGC ACAGGAGCAT TTCCTGGGCA GACTCTGCGG TGGTTCTGGA GGAGGGAAAT
    TCAAGCGAGA CCAGTCTGCC AGTGGAGCAC ACGGAGCTGG AGGACTTCGA GGCGACACTA
    GGCACAGACC GGCGCTGTGG CCGCGCGGAA GCTTACAGCA GGTCGTCTTC AAGCTCCAGC
    CAGGAGGAGA AGTTCCCTGC AGAGGAGCTG GCTGCCGGAG GGATCCCCAT CGAACGAGCT
    GTCTCTTCCG AGGGTGCCCG GCCTTCTGTG GAGCTCTCCT TCCAGCCCTC CCAGCCCCTC
    AGCAAGTCCA GCTCCTCCCC TGAGCTGCAG ACCCTGCAGG ACATACTCGG GGACCCCGGG
    GACAAGTCTG ATGTTGGCCG GCTCAGCCCT GAGGCCAAGG CCCGGTCACA GTCCGGGATT
    CTGGACGGGG TGGGTGCCTC CTGGTCAGCC CCAGGTGAAG AGAACCAGGG CCAGGGCCCC
    GCACAGCCCG AGGGTCCCTT GCCTTCCAGC TGCCCCCGCT CCCCCAGTGG CCTGCGGCCC
    AGAGGCTACA CCATCTCTGA TTCAGCCCCG TCACGCAGAG GCAAGAGGGT AGAGCGGGAC
    GCCTTCAGGA GCAGAGCTGG GGCTTCCAAC ACTGAGAAGG TGCCAGGCAT CAATCCCAGC
    TTTGTGTTCC TGCAGCTCTA CCACTCACCC TTCTTTGGTG ACGAGTCCAA CAAGCCCATC
    CTTCTACCCA ATGAGTCCTT CGAGCGGTCA GTGCAGCTTC TCGACCAGAT TCCATCATAC
    GACACACACA AGATTGCCGT CCTGTACGTG GGAGAAGGCC AGAGCAGCAG TGAGCTCGCC
    ATCCTGTCCA ATGAGCATGG CTCGTACAGG TACACGGAGT TCCTGACAGG CCTGGGCAAG
    CTCATTGAGC TCAAAGACTG CCAGCCGGAC AAGGTGTACC TGGGGGGCCT GGACGTGTGT
    GGCGAAGATG GCCAGTTCAC CTACTGCTGG CATGACGACA TCATGCAAGC CGTCTTCCAC
    ATTGCTACCC TGATGCCCAC CAAGGAAGTG GACAAGCACC GCTGTGACAA GAAGCGCCAC
    CTGGGCAATG ACTTTGTGTC CATCGTCTAC AACGACTCTG GTGAGGACTT CAAGCTGGGC
    ACCATCCGAG GCCAGTTCAA CTTCGTCCAC GTGATCATCA CCCCCCTGGA CTATGAGTGC
    AACCTGGTGT CCCTGCAGTG CAGGAAAGAC ATGGAAGGTC TTGTGGATAC TAGCGTGGCC
    AAGATCGTGT CTGACCGCAA CTTGCCTTTT GTGGCACGTC AGATGGCTTT GCATGCAAAT
    ATGGCCTCAC AAGTGCATCA TAGCCGCTCC AACCCCACCG ACATCTATCC CTCCAAGTGG
    ATTGCCAGGC TTCGCCACAT CAAGCGACTC CGCCACCGGA TCCGCGAGGA AGCCCACTAC
    ACCAACCCCA GCCTGCCACT GATGCAGATG CACCCCCCGG GCCACCCCAA AGCCTCTGCT
    CAGGCGCCGG CAGAGCCTGT GCCCACGTAT GAGACAGGCC AGCGGAAGCG TCTCATCTCC
    TCTGTGGATG ACTTCACGGA GTTTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe08335
    Clone ID Related Accession (Same CDS sequence) XM_005621720.2 , XM_005621720.3
    Accession Version XM_005621720.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5244bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product tuberin isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005621720.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
    CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC
    ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG
    ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG
    CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA
    GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG
    GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG
    GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG
    GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT
    GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC
    ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC
    GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG
    CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG
    GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG
    ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG
    CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC
    TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC
    CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC
    AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC
    CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG
    TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC
    TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT
    GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA
    GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT
    CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG
    AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC
    AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT
    AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG
    CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC
    AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT
    GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG
    CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG
    GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG
    GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG
    CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC
    GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG
    GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC
    GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC
    ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC
    GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG
    TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC
    CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC
    CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC
    AACGAGAGGC CCAAGAGCAG GATCCAGACG TCCCTCACAA GCGCCAGCTT GGGGTCTGCT
    GACGAGAACT CGATGGCCCA GGCTGATGAC AACTTGAAAA ATCTCCACCT GGAGCTCACG
    GAAACATGTC TGGACATGAT GGCCAGATAT GTGTTCTCCA ACTTCACAGC AGTCCCCAAG
    AGGTCCCCGG TGGGAGAGTT CCTCCTGGCT GGTGGCAGGA CCAAAACCTG GCTGGTTGGG
    AACAAGCTTG TCACTGTGAC GACGAGCGTG GGGACGGGGA CCCGGTCGCT GCTGGGCTTG
    GACTCAGGAG ATCAGCAAGG TGGCCCCGAG TTGAGCTCGG ACCCTGGCAT GCATGTGAGA
    CAGGCAAAGG AGGCACCTGC CAAGCTGGAG TCGCAGGCTG GGCAGCAGGT GTGCCGCGGC
    GCCCGGGACC GGGTCCGCTC CATGTCCGGG GGCCACGGTC TTCGTGTTGG CGCCCTGGAC
    ACTCCAGCCT CCCACTTGCC GGGTGGCCCC ACTTCCCCAG GCACGCAGGC AGCGCTGGCC
    AGCAAGTCTG ACAGGGCCTC GGCTGGCACC CGGTTCCCAG CACAGGAGAA GACGAGCCTG
    GCAGCCTACG TACCGCTGCT GACCCAGGGC TGGGCGGAGA TCTTGGTCCG GAGGCCTACA
    GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCCTTCTC GTCGGACATC
    AACAACATGC CTCTGCAGGA GCTGTCCAAC GCGCTCATGG CCGCTGAGCG CTTCAAGGAG
    CGTCGTGACA CGGCTCTGTA CAAGTCGCTG TCGGTGCCTG CCGCAGGCTC GGCCAAGCCC
    CCTCCGCCCC CGAGATCTAA CACGGACTCT GCGGTGGTTC TGGAGGAGGG AAATTCAAGC
    GAGACCAGTC TGCCAGTGGA GCACACGGAG CTGGAGGACT TCGAGGCGAC ACTAGGCACA
    GACCGGCGCT GTGGCCGCGC GGAAGCTTAC AGCAGGTCGT CTTCAAGCTC CAGCCAGGAG
    GAGAAGTTCC CTGCAGAGGA GCTGGCTGCC GGAGGGATCC CCATCGAACG AGCTGTCTCT
    TCCGAGGGTG CCCGGCCTTC TGTGGAGCTC TCCTTCCAGC CCTCCCAGCC CCTCAGCAAG
    TCCAGCTCCT CCCCTGAGCT GCAGACCCTG CAGGACATAC TCGGGGACCC CGGGGACAAG
    TCTGATGTTG GCCGGCTCAG CCCTGAGGCC AAGGCCCGGT CACAGTCCGG GATTCTGGAC
    GGGGTGGGTG CCTCCTGGTC AGCCCCAGGT GAAGAGAACC AGGGCCAGGG CCCCGCACAG
    CCCGAGGGTC CCTTGCCTTC CAGCTGCCCC CGCTCCCCCA GTGGCCTGCG GCCCAGAGGC
    TACACCATCT CTGATTCAGC CCCGTCACGC AGAGGCAAGA GGGTAGAGCG GGACGCCTTC
    AGGAGCAGAG CTGGGGCTTC CAACACTGAG AAGGTGCCAG GCATCAATCC CAGCTTTGTG
    TTCCTGCAGC TCTACCACTC ACCCTTCTTT GGTGACGAGT CCAACAAGCC CATCCTTCTA
    CCCAATGAGT CCTTCGAGCG GTCAGTGCAG CTTCTCGACC AGATTCCATC ATACGACACA
    CACAAGATTG CCGTCCTGTA CGTGGGAGAA GGCCAGAGCA GCAGTGAGCT CGCCATCCTG
    TCCAATGAGC ATGGCTCGTA CAGGTACACG GAGTTCCTGA CAGGCCTGGG CAAGCTCATT
    GAGCTCAAAG ACTGCCAGCC GGACAAGGTG TACCTGGGGG GCCTGGACGT GTGTGGCGAA
    GATGGCCAGT TCACCTACTG CTGGCATGAC GACATCATGC AAGCCGTCTT CCACATTGCT
    ACCCTGATGC CCACCAAGGA AGTGGACAAG CACCGCTGTG ACAAGAAGCG CCACCTGGGC
    AATGACTTTG TGTCCATCGT CTACAACGAC TCTGGTGAGG ACTTCAAGCT GGGCACCATC
    CGAGGCCAGT TCAACTTCGT CCACGTGATC ATCACCCCCC TGGACTATGA GTGCAACCTG
    GTGTCCCTGC AGTGCAGGAA AGACATGGAA GGTCTTGTGG ATACTAGCGT GGCCAAGATC
    GTGTCTGACC GCAACTTGCC TTTTGTGGCA CGTCAGATGG CTTTGCATGC AAATATGGCC
    TCACAAGTGC ATCATAGCCG CTCCAACCCC ACCGACATCT ATCCCTCCAA GTGGATTGCC
    AGGCTTCGCC ACATCAAGCG ACTCCGCCAC CGGATCCGCG AGGAAGCCCA CTACACCAAC
    CCCAGCCTGC CACTGATGCA GATGCACCCC CCGGGCCACC CCAAAGCCTC TGCTCAGGCG
    CCGGCAGAGC CTGTGCCCAC GTATGAGACA GGCCAGCGGA AGCGTCTCAT CTCCTCTGTG
    GATGACTTCA CGGAGTTTGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005621777.1
    CDS282..5525
    Translation

    Target ORF information:

    RefSeq Version XM_005621720.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris tuberous sclerosis 2 (TSC2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005621720.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
    CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC
    ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG
    ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG
    CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA
    GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG
    GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG
    GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG
    GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT
    GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC
    ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC
    GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG
    CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG
    GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG
    ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG
    CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC
    TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC
    CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC
    AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC
    CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG
    TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC
    TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT
    GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA
    GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT
    CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG
    AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC
    AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT
    AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG
    CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC
    AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT
    GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG
    CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG
    GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG
    GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG
    CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC
    GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG
    GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC
    GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC
    ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC
    GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG
    TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC
    CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC
    CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC
    AACGAGAGGC CCAAGAGCAG GATCCAGACG TCCCTCACAA GCGCCAGCTT GGGGTCTGCT
    GACGAGAACT CGATGGCCCA GGCTGATGAC AACTTGAAAA ATCTCCACCT GGAGCTCACG
    GAAACATGTC TGGACATGAT GGCCAGATAT GTGTTCTCCA ACTTCACAGC AGTCCCCAAG
    AGGTCCCCGG TGGGAGAGTT CCTCCTGGCT GGTGGCAGGA CCAAAACCTG GCTGGTTGGG
    AACAAGCTTG TCACTGTGAC GACGAGCGTG GGGACGGGGA CCCGGTCGCT GCTGGGCTTG
    GACTCAGGAG ATCAGCAAGG TGGCCCCGAG TTGAGCTCGG ACCCTGGCAT GCATGTGAGA
    CAGGCAAAGG AGGCACCTGC CAAGCTGGAG TCGCAGGCTG GGCAGCAGGT GTGCCGCGGC
    GCCCGGGACC GGGTCCGCTC CATGTCCGGG GGCCACGGTC TTCGTGTTGG CGCCCTGGAC
    ACTCCAGCCT CCCACTTGCC GGGTGGCCCC ACTTCCCCAG GCACGCAGGC AGCGCTGGCC
    AGCAAGTCTG ACAGGGCCTC GGCTGGCACC CGGTTCCCAG CACAGGAGAA GACGAGCCTG
    GCAGCCTACG TACCGCTGCT GACCCAGGGC TGGGCGGAGA TCTTGGTCCG GAGGCCTACA
    GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCCTTCTC GTCGGACATC
    AACAACATGC CTCTGCAGGA GCTGTCCAAC GCGCTCATGG CCGCTGAGCG CTTCAAGGAG
    CGTCGTGACA CGGCTCTGTA CAAGTCGCTG TCGGTGCCTG CCGCAGGCTC GGCCAAGCCC
    CCTCCGCCCC CGAGATCTAA CACGGACTCT GCGGTGGTTC TGGAGGAGGG AAATTCAAGC
    GAGACCAGTC TGCCAGTGGA GCACACGGAG CTGGAGGACT TCGAGGCGAC ACTAGGCACA
    GACCGGCGCT GTGGCCGCGC GGAAGCTTAC AGCAGGTCGT CTTCAAGCTC CAGCCAGGAG
    GAGAAGTTCC CTGCAGAGGA GCTGGCTGCC GGAGGGATCC CCATCGAACG AGCTGTCTCT
    TCCGAGGGTG CCCGGCCTTC TGTGGAGCTC TCCTTCCAGC CCTCCCAGCC CCTCAGCAAG
    TCCAGCTCCT CCCCTGAGCT GCAGACCCTG CAGGACATAC TCGGGGACCC CGGGGACAAG
    TCTGATGTTG GCCGGCTCAG CCCTGAGGCC AAGGCCCGGT CACAGTCCGG GATTCTGGAC
    GGGGTGGGTG CCTCCTGGTC AGCCCCAGGT GAAGAGAACC AGGGCCAGGG CCCCGCACAG
    CCCGAGGGTC CCTTGCCTTC CAGCTGCCCC CGCTCCCCCA GTGGCCTGCG GCCCAGAGGC
    TACACCATCT CTGATTCAGC CCCGTCACGC AGAGGCAAGA GGGTAGAGCG GGACGCCTTC
    AGGAGCAGAG CTGGGGCTTC CAACACTGAG AAGGTGCCAG GCATCAATCC CAGCTTTGTG
    TTCCTGCAGC TCTACCACTC ACCCTTCTTT GGTGACGAGT CCAACAAGCC CATCCTTCTA
    CCCAATGAGT CCTTCGAGCG GTCAGTGCAG CTTCTCGACC AGATTCCATC ATACGACACA
    CACAAGATTG CCGTCCTGTA CGTGGGAGAA GGCCAGAGCA GCAGTGAGCT CGCCATCCTG
    TCCAATGAGC ATGGCTCGTA CAGGTACACG GAGTTCCTGA CAGGCCTGGG CAAGCTCATT
    GAGCTCAAAG ACTGCCAGCC GGACAAGGTG TACCTGGGGG GCCTGGACGT GTGTGGCGAA
    GATGGCCAGT TCACCTACTG CTGGCATGAC GACATCATGC AAGCCGTCTT CCACATTGCT
    ACCCTGATGC CCACCAAGGA AGTGGACAAG CACCGCTGTG ACAAGAAGCG CCACCTGGGC
    AATGACTTTG TGTCCATCGT CTACAACGAC TCTGGTGAGG ACTTCAAGCT GGGCACCATC
    CGAGGCCAGT TCAACTTCGT CCACGTGATC ATCACCCCCC TGGACTATGA GTGCAACCTG
    GTGTCCCTGC AGTGCAGGAA AGACATGGAA GGTCTTGTGG ATACTAGCGT GGCCAAGATC
    GTGTCTGACC GCAACTTGCC TTTTGTGGCA CGTCAGATGG CTTTGCATGC AAATATGGCC
    TCACAAGTGC ATCATAGCCG CTCCAACCCC ACCGACATCT ATCCCTCCAA GTGGATTGCC
    AGGCTTCGCC ACATCAAGCG ACTCCGCCAC CGGATCCGCG AGGAAGCCCA CTACACCAAC
    CCCAGCCTGC CACTGATGCA GATGCACCCC CCGGGCCACC CCAAAGCCTC TGCTCAGGCG
    CCGGCAGAGC CTGTGCCCAC GTATGAGACA GGCCAGCGGA AGCGTCTCAT CTCCTCTGTG
    GATGACTTCA CGGAGTTTGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe08335
    Clone ID Related Accession (Same CDS sequence) XM_005621720.2 , XM_005621720.3
    Accession Version XM_005621720.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5244bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product tuberin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005621720.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
    CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC
    ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG
    ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG
    CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA
    GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG
    GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG
    GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG
    GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT
    GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC
    ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC
    GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG
    CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG
    GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG
    ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG
    CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC
    TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC
    CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC
    AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC
    CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG
    TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC
    TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT
    GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA
    GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT
    CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG
    AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC
    AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT
    AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG
    CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC
    AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT
    GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG
    CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG
    GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG
    GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG
    CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC
    GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG
    GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC
    GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC
    ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC
    GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG
    TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC
    CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC
    CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC
    AACGAGAGGC CCAAGAGCAG GATCCAGACG TCCCTCACAA GCGCCAGCTT GGGGTCTGCT
    GACGAGAACT CGATGGCCCA GGCTGATGAC AACTTGAAAA ATCTCCACCT GGAGCTCACG
    GAAACATGTC TGGACATGAT GGCCAGATAT GTGTTCTCCA ACTTCACAGC AGTCCCCAAG
    AGGTCCCCGG TGGGAGAGTT CCTCCTGGCT GGTGGCAGGA CCAAAACCTG GCTGGTTGGG
    AACAAGCTTG TCACTGTGAC GACGAGCGTG GGGACGGGGA CCCGGTCGCT GCTGGGCTTG
    GACTCAGGAG ATCAGCAAGG TGGCCCCGAG TTGAGCTCGG ACCCTGGCAT GCATGTGAGA
    CAGGCAAAGG AGGCACCTGC CAAGCTGGAG TCGCAGGCTG GGCAGCAGGT GTGCCGCGGC
    GCCCGGGACC GGGTCCGCTC CATGTCCGGG GGCCACGGTC TTCGTGTTGG CGCCCTGGAC
    ACTCCAGCCT CCCACTTGCC GGGTGGCCCC ACTTCCCCAG GCACGCAGGC AGCGCTGGCC
    AGCAAGTCTG ACAGGGCCTC GGCTGGCACC CGGTTCCCAG CACAGGAGAA GACGAGCCTG
    GCAGCCTACG TACCGCTGCT GACCCAGGGC TGGGCGGAGA TCTTGGTCCG GAGGCCTACA
    GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCCTTCTC GTCGGACATC
    AACAACATGC CTCTGCAGGA GCTGTCCAAC GCGCTCATGG CCGCTGAGCG CTTCAAGGAG
    CGTCGTGACA CGGCTCTGTA CAAGTCGCTG TCGGTGCCTG CCGCAGGCTC GGCCAAGCCC
    CCTCCGCCCC CGAGATCTAA CACGGACTCT GCGGTGGTTC TGGAGGAGGG AAATTCAAGC
    GAGACCAGTC TGCCAGTGGA GCACACGGAG CTGGAGGACT TCGAGGCGAC ACTAGGCACA
    GACCGGCGCT GTGGCCGCGC GGAAGCTTAC AGCAGGTCGT CTTCAAGCTC CAGCCAGGAG
    GAGAAGTTCC CTGCAGAGGA GCTGGCTGCC GGAGGGATCC CCATCGAACG AGCTGTCTCT
    TCCGAGGGTG CCCGGCCTTC TGTGGAGCTC TCCTTCCAGC CCTCCCAGCC CCTCAGCAAG
    TCCAGCTCCT CCCCTGAGCT GCAGACCCTG CAGGACATAC TCGGGGACCC CGGGGACAAG
    TCTGATGTTG GCCGGCTCAG CCCTGAGGCC AAGGCCCGGT CACAGTCCGG GATTCTGGAC
    GGGGTGGGTG CCTCCTGGTC AGCCCCAGGT GAAGAGAACC AGGGCCAGGG CCCCGCACAG
    CCCGAGGGTC CCTTGCCTTC CAGCTGCCCC CGCTCCCCCA GTGGCCTGCG GCCCAGAGGC
    TACACCATCT CTGATTCAGC CCCGTCACGC AGAGGCAAGA GGGTAGAGCG GGACGCCTTC
    AGGAGCAGAG CTGGGGCTTC CAACACTGAG AAGGTGCCAG GCATCAATCC CAGCTTTGTG
    TTCCTGCAGC TCTACCACTC ACCCTTCTTT GGTGACGAGT CCAACAAGCC CATCCTTCTA
    CCCAATGAGT CCTTCGAGCG GTCAGTGCAG CTTCTCGACC AGATTCCATC ATACGACACA
    CACAAGATTG CCGTCCTGTA CGTGGGAGAA GGCCAGAGCA GCAGTGAGCT CGCCATCCTG
    TCCAATGAGC ATGGCTCGTA CAGGTACACG GAGTTCCTGA CAGGCCTGGG CAAGCTCATT
    GAGCTCAAAG ACTGCCAGCC GGACAAGGTG TACCTGGGGG GCCTGGACGT GTGTGGCGAA
    GATGGCCAGT TCACCTACTG CTGGCATGAC GACATCATGC AAGCCGTCTT CCACATTGCT
    ACCCTGATGC CCACCAAGGA AGTGGACAAG CACCGCTGTG ACAAGAAGCG CCACCTGGGC
    AATGACTTTG TGTCCATCGT CTACAACGAC TCTGGTGAGG ACTTCAAGCT GGGCACCATC
    CGAGGCCAGT TCAACTTCGT CCACGTGATC ATCACCCCCC TGGACTATGA GTGCAACCTG
    GTGTCCCTGC AGTGCAGGAA AGACATGGAA GGTCTTGTGG ATACTAGCGT GGCCAAGATC
    GTGTCTGACC GCAACTTGCC TTTTGTGGCA CGTCAGATGG CTTTGCATGC AAATATGGCC
    TCACAAGTGC ATCATAGCCG CTCCAACCCC ACCGACATCT ATCCCTCCAA GTGGATTGCC
    AGGCTTCGCC ACATCAAGCG ACTCCGCCAC CGGATCCGCG AGGAAGCCCA CTACACCAAC
    CCCAGCCTGC CACTGATGCA GATGCACCCC CCGGGCCACC CCAAAGCCTC TGCTCAGGCG
    CCGGCAGAGC CTGTGCCCAC GTATGAGACA GGCCAGCGGA AGCGTCTCAT CTCCTCTGTG
    GATGACTTCA CGGAGTTTGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005621777.1
    CDS289..5532
    Translation

    Target ORF information:

    RefSeq Version XM_005621720.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris TSC complex subunit 2 (TSC2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005621720.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCCAAGC CTACGAGCAA AGACTCAGGC CTGAAGGAGA AGTTCAGGAT TCTCTTGGGA 
    CTGGGAACCC CGAGGCCAAA CCCCAGGTCA GCAGAGGGCA AGCAGACCGA GTTCATCATC
    ACGGCGGACA TCCTGAGAGA GCTGAGTGTT GAATGCGGCA TCAACAATCG CATCCGGGTG
    ATAGGGCAGA TCTGCGAAGT TGCAAAAACG AAGAAATTTG AAGAGCATGC AGTGGAAGCG
    CTCTGGAAGG CTGTCGCGGA CCTGCTGCAG CCGGAGCGTC CCCCCGAGGC CCGGCATGCA
    GTGCTGGCGC TGCTGAAGGC CATTGTGCAG GGGCAGGGGG ACCGCCTGGG CGTTCTCCGG
    GCTCTGTTCT TTAGGGCCAT CAAGGATTAC CCCGCTAACG AAGACCTCCA TGAAAGACTG
    GAAGTTTTCA AGGCCCTCAC GGACAATGGG AGACACATCA CCTACTTGGA AGAAGAACTG
    GCTGGGTTTG TCCTGCAGTG GATGGACATC GGCCTGTCCT CCGAATTCCT CCTGGTGCTT
    GTGAACTTGG TCAAGTTCAA CAGCTGTTAC CTGGATGAGT ACATTGCATC AATGGTCCAC
    ATGATCTGCC TGCTGTGCGT CCAGACCGTG TCATCCGTGG ACATAGAGGT CTCCCTGCAG
    GTGCTGGACG CCGTCGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC CCTGTTCATC
    GTCACCCTGT GCCGCACCAT CAACGTGAAG GAGCTCTGTG AGCCTTGCTG GAAGCTGATG
    CGGAACCTGC TGGGCACCCA CCTGGGCCAC AGTGCCATCT ACAACATGTG TCGCATCATG
    GAGGACAGAG CCTACATGGA AGATGCCCCA CTGCTGAGAG GGGCTGTGTT CTTTGTGGGG
    ATGGCCCTTT GGGGAGCACA CCGACTCTAC TCTCTCAAGA ACTCCCCAAC GTCTGTGTTG
    CCGTCGTTTT ATGAGGCTAT GACCTGTCCG AATGAAGTGG TGTCCTATGA AATCGTCCTC
    TCTATAACCA GACTCATCAA GAAGTACCGG AGGGAGCTGC AAGCTGTGAC CTGGGACATC
    CTACTGAACA TCATGGAGCG CCTCCTGCAC CAGCTCCAGA GCCTGGACAG CCCGGAGCTC
    AGAGCCATCG TGCACGACCT GCTGACCACA GTGGAAGAGC TGTGTGATCA GAATGAGTTC
    CACGGCTCTC AGGAGAGATA CTTTGAGCTG GTTGAGCGAT GTGCAGACCA GAGGCCGGAG
    TCTTCCCTCT TAAACTTAAT AACGTACCGG GCTCAGTCAA TCCACCCGGC GAAGGATGGC
    TGGATTCAGA ACCTGCAGTT ACTGATGGAG AGGTTCTTCA GGAACGAATC TCGCAGTGCT
    GTGCGCATCA AGGTGCTGGA CGTCCTGTCC TTCGTGCTGC TCATCAACAG GCAGTTCTAT
    GAGGAGGAGC TGATCAGCTC GGTGGTCATC TCACAGCTCT CCCATGTCCC GGAGGACAAA
    GACCCCCAAG TCCGGAAACT GGCCACCCAG TTGCTGGTGG ACCTGGCCGA AGGCTGCCAC
    ACCCACCACT TCAACAGTCT GTTGGACATT GTGGAAAAGG TGATTGCCCG CTCTCTCTCT
    CCACCCCCTG AGCTGGAGGA AAGAGACGTG GCGGTGTACT CGGCCTCCTT AGAGGATGTG
    AAGACTGCTG TCCTGGGGCT CCTGGTCATC CTTCAGACCA AGCTGTACGC CTTGCCTGCC
    AGCCATGCGA TGCGCGTGTA CGAGACACTC GTCAGCCACA TCCAGCTCCA CTACAAGCAT
    AGCTATACCC TGCCCATTGC CAGTAGCATC CGGCTGCAGG CTTTTGACTT TCTACTGCTG
    CTGAGGGCCG ACTCGCTGCA CCGCCTCGGC CTGCCCAGCA AGGACGGGGC CGTGAGGTTC
    AGCCCTTACT GCGTCTGCGA TGCCATGGAG CCAGAGAGAG GCTCAGAGAA GAAGGCCAGT
    GGCCCCCTGT CACCTCCCAC GGGGCCTCCT GGTCCTGCGC CCGCAGGCCC CGCCGTGCGG
    CTTGGCTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCCT GAAGCTGGAG
    GTGGACTGGA AGGTACTGAA GCTCGTGCTT AGCAAGCTGC CAGAGTCCCT GCGTTATAAG
    GTGCTCATCT TCACCTCGCC CTGCAGCGTG GACCAGCTGT CCTCCGCCCT CTGCTCCATG
    CTCTCAGGTC CCAGAACTCT GGAGCGGCTC CGAGGCACCC CGGAAGGGTT CTCCAGAACC
    GACCTGCACC TGGCTGTGGT CCCTGTGCTG ACGGCGCTCA TCTCTTACCA TAACTACCTG
    GACAAAACCA GACAGCGGGA GATGGTGTAC TGCCTGGAGC AAGGCCTGAT CTACCGCTGC
    GCCAGCCAGT GTGTGGTGGC CCTGGCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC
    AAGGCGCTTC CCGTCCTGGT CGTGAAGCTC ACACACATCT CGGCCACGGC CAGTATGGCC
    ATTCCACTCC TCGAGTTCCT GTCAACGCTG GCCAGGTTGC CCCACCTGTA CAGGAACTTC
    GCAGCAGAGC AGTACGCGAG TGTGTTTGCC ATCTCCCTGC CCTACACCAA TCCCTCCAAG
    TTCAATCAGT ACATCGTGTG CCTGGCCCAT CATGTCATAG CCATGTGGTT CATTAGATGC
    CGCCTGCCCT TCCGGAAGGA TTTTGTTCCT TATATCACTA AGGGCCTGCG CTCCAACGTC
    CTCCTGTCTT TTGATGACAC CCCCGAGAAG GACAGTTTCA GAGCACGAAG CACCAGTCTC
    AACGAGAGGC CCAAGAGCAG GATCCAGACG TCCCTCACAA GCGCCAGCTT GGGGTCTGCT
    GACGAGAACT CGATGGCCCA GGCTGATGAC AACTTGAAAA ATCTCCACCT GGAGCTCACG
    GAAACATGTC TGGACATGAT GGCCAGATAT GTGTTCTCCA ACTTCACAGC AGTCCCCAAG
    AGGTCCCCGG TGGGAGAGTT CCTCCTGGCT GGTGGCAGGA CCAAAACCTG GCTGGTTGGG
    AACAAGCTTG TCACTGTGAC GACGAGCGTG GGGACGGGGA CCCGGTCGCT GCTGGGCTTG
    GACTCAGGAG ATCAGCAAGG TGGCCCCGAG TTGAGCTCGG ACCCTGGCAT GCATGTGAGA
    CAGGCAAAGG AGGCACCTGC CAAGCTGGAG TCGCAGGCTG GGCAGCAGGT GTGCCGCGGC
    GCCCGGGACC GGGTCCGCTC CATGTCCGGG GGCCACGGTC TTCGTGTTGG CGCCCTGGAC
    ACTCCAGCCT CCCACTTGCC GGGTGGCCCC ACTTCCCCAG GCACGCAGGC AGCGCTGGCC
    AGCAAGTCTG ACAGGGCCTC GGCTGGCACC CGGTTCCCAG CACAGGAGAA GACGAGCCTG
    GCAGCCTACG TACCGCTGCT GACCCAGGGC TGGGCGGAGA TCTTGGTCCG GAGGCCTACA
    GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCCTTCTC GTCGGACATC
    AACAACATGC CTCTGCAGGA GCTGTCCAAC GCGCTCATGG CCGCTGAGCG CTTCAAGGAG
    CGTCGTGACA CGGCTCTGTA CAAGTCGCTG TCGGTGCCTG CCGCAGGCTC GGCCAAGCCC
    CCTCCGCCCC CGAGATCTAA CACGGACTCT GCGGTGGTTC TGGAGGAGGG AAATTCAAGC
    GAGACCAGTC TGCCAGTGGA GCACACGGAG CTGGAGGACT TCGAGGCGAC ACTAGGCACA
    GACCGGCGCT GTGGCCGCGC GGAAGCTTAC AGCAGGTCGT CTTCAAGCTC CAGCCAGGAG
    GAGAAGTTCC CTGCAGAGGA GCTGGCTGCC GGAGGGATCC CCATCGAACG AGCTGTCTCT
    TCCGAGGGTG CCCGGCCTTC TGTGGAGCTC TCCTTCCAGC CCTCCCAGCC CCTCAGCAAG
    TCCAGCTCCT CCCCTGAGCT GCAGACCCTG CAGGACATAC TCGGGGACCC CGGGGACAAG
    TCTGATGTTG GCCGGCTCAG CCCTGAGGCC AAGGCCCGGT CACAGTCCGG GATTCTGGAC
    GGGGTGGGTG CCTCCTGGTC AGCCCCAGGT GAAGAGAACC AGGGCCAGGG CCCCGCACAG
    CCCGAGGGTC CCTTGCCTTC CAGCTGCCCC CGCTCCCCCA GTGGCCTGCG GCCCAGAGGC
    TACACCATCT CTGATTCAGC CCCGTCACGC AGAGGCAAGA GGGTAGAGCG GGACGCCTTC
    AGGAGCAGAG CTGGGGCTTC CAACACTGAG AAGGTGCCAG GCATCAATCC CAGCTTTGTG
    TTCCTGCAGC TCTACCACTC ACCCTTCTTT GGTGACGAGT CCAACAAGCC CATCCTTCTA
    CCCAATGAGT CCTTCGAGCG GTCAGTGCAG CTTCTCGACC AGATTCCATC ATACGACACA
    CACAAGATTG CCGTCCTGTA CGTGGGAGAA GGCCAGAGCA GCAGTGAGCT CGCCATCCTG
    TCCAATGAGC ATGGCTCGTA CAGGTACACG GAGTTCCTGA CAGGCCTGGG CAAGCTCATT
    GAGCTCAAAG ACTGCCAGCC GGACAAGGTG TACCTGGGGG GCCTGGACGT GTGTGGCGAA
    GATGGCCAGT TCACCTACTG CTGGCATGAC GACATCATGC AAGCCGTCTT CCACATTGCT
    ACCCTGATGC CCACCAAGGA AGTGGACAAG CACCGCTGTG ACAAGAAGCG CCACCTGGGC
    AATGACTTTG TGTCCATCGT CTACAACGAC TCTGGTGAGG ACTTCAAGCT GGGCACCATC
    CGAGGCCAGT TCAACTTCGT CCACGTGATC ATCACCCCCC TGGACTATGA GTGCAACCTG
    GTGTCCCTGC AGTGCAGGAA AGACATGGAA GGTCTTGTGG ATACTAGCGT GGCCAAGATC
    GTGTCTGACC GCAACTTGCC TTTTGTGGCA CGTCAGATGG CTTTGCATGC AAATATGGCC
    TCACAAGTGC ATCATAGCCG CTCCAACCCC ACCGACATCT ATCCCTCCAA GTGGATTGCC
    AGGCTTCGCC ACATCAAGCG ACTCCGCCAC CGGATCCGCG AGGAAGCCCA CTACACCAAC
    CCCAGCCTGC CACTGATGCA GATGCACCCC CCGGGCCACC CCAAAGCCTC TGCTCAGGCG
    CCGGCAGAGC CTGTGCCCAC GTATGAGACA GGCCAGCGGA AGCGTCTCAT CTCCTCTGTG
    GATGACTTCA CGGAGTTTGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.