UBE4B cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	UBE4B
Entrez Gene ID	479603
Full Name	ubiquitination factor E4B
General protein information	Preferred Names ubiquitination factor E4B Names ubiquitin conjugation factor E4 B ubiquitination factor E4B (UFD2 homolog, yeast)
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005620527.3	XP_005620584.1	ubiquitin conjugation factor E4 B isoform X3
XM_844731.4	XP_849824.2	ubiquitin conjugation factor E4 B isoform X3
XM_005620526.3	XP_005620583.1	ubiquitin conjugation factor E4 B isoform X1
XM_844731.5	XP_849824.2	ubiquitin conjugation factor E4 B isoform X4
XM_005620526.2	XP_005620583.1	ubiquitin conjugation factor E4 B isoform X1
XM_005620527.2	XP_005620584.1	ubiquitin conjugation factor E4 B isoform X2
XM_022419040.1	XP_022274748.1	ubiquitin conjugation factor E4 B isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	UBE4B	XP_001161561.1
Kluyveromyces lactis	KLLA0F05269g	XP_455319.1
Eremothecium gossypii	AGOS_ADR096C	NP_984192.1
Neurospora crassa	NCU03357	XP_956466.1
Caenorhabditis elegans (roundworm)	ufd-2	NP_495691.1
Schizosaccharomyces pombe (fission yeast)	ufd2	NP_593630.1
Rattus norvegicus (Norway rat)	Ube4b	NP_001258127.1
Drosophila melanogaster (fruit fly)	CG9934	NP_609597.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007870	XP_317622.4
Magnaporthe oryzae (rice blast fungus)	MGG_03255	XP_003716719.1
Arabidopsis thaliana (thale cress)	AT5G15400	NP_568313.2
Mus musculus (house mouse)	Ube4b	NP_071305.2
Danio rerio (zebrafish)	ube4b	NP_919343.1
Xenopus tropicalis (tropical clawed frog)	ube4b	XP_004916084.1
Homo sapiens (human)	UBE4B	NP_001099032.1
	UBE4B	XP_001101836.1
Canis lupus familiaris (dog)	UBE4B	XP_005620583.1
Bos taurus (cattle)	UBE4B	NP_001095974.1
Gallus gallus (chicken)	UBE4B	XP_004947510.1
Saccharomyces cerevisiae (baker's yeast)	UFD2	NP_010091.2

Gene Ontology

The following UBE4B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBE4B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe288433	XM_022419040.1 Latest version!	Canis lupus familiaris ubiquitination factor E4B (UBE4B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe06877	XM_005620527.3 Latest version!	Canis lupus familiaris ubiquitination factor E4B (UBE4B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OCe06878	XM_844731.5 Latest version!	Canis lupus familiaris ubiquitination factor E4B (UBE4B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OCe06876	XM_005620526.3 Latest version!	Canis lupus familiaris ubiquitination factor E4B (UBE4B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
OCe06878	XM_844731.4	Canis lupus familiaris ubiquitination factor E4B (UBE4B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OCe06876	XM_005620526.2	Canis lupus familiaris ubiquitination factor E4B (UBE4B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OCe06877	XM_005620527.2	Canis lupus familiaris ubiquitination factor E4B (UBE4B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBE4B ( XM_022419040.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022274748.1 Canis lupus familiaris ubiquitination factor E4B (UBE4B), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe288433
Clone ID Related Accession (Same CDS sequence)	XM_022419040.1
Accession Version	XM_022419040.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	ubiquitin conjugation factor E4 B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGAGCGCTGA TGAGATCCGA CGGAGGCGCC TTGCACGACT TGCTGGTGGA 
CAGACCTCCC AGCCAACCAC CCCACTCACC TCGCCCCAGA GGGAGAACCC TCTGGGGCCT 
CCTATCGCAG CATCAGCCCC AGGCCCTTCC CAGAGCCTTG GTCTCAATGT CCACAACATG 
ACCCCAGCTA CCTCCCCTAT AGGTGCATCA GGAGTAGCTC ACCGAAGCCA GAGCAGTGAA 
GGAGTCAGTT CTCTCAGCAG CTCGCCCTCC AATAGCCTTG AAACCCAATC TCAGTCTCTC 
TCACGTTCCC AGAGCATGGA TATCGATGGG GTCTCTTGTG AAAAAAGCAT GTCCCAGGTG 
GATGTGGATT CAGGAATAGA AAATATGGAG GTTGATGAAA ACGATCGAAG AGAAAAGAGG 
AGCCTCAGCG ATAAGGAGCC TTCCTCGGGT TCTGAAGTAT CTGAAGAACA GGCCTTACAG 
CTGGTCTGTA AGATCTTTCG TGTCTCCTGG AAGGACCGGG ACAGAGATGT CATCTTTCTT 
TCTTCTCTTT CTGCACAATT TAAGCAGAAC CCAAAAGAAG TGTTCTCTGA TTTTAAGGAC 
TTGATTGGCC AGATTTTAAT GGAAGTGCTA ATGATGTCTA CGCAGACTAG AGATGAAAAT 
CCATTTGCCA GTCTGACAGC CACATCACAG CCAATTGCAG CAGCAGCTCG GTCACCAGAC 
AGGAATCTAA TGCTCACTAC TGGCTCTAAC CCAGGAACAA GCCCCATGTT CTGTGGTGTG 
GGTTCCTTTG GTGCCAGCTC TTTCTCTAGC CTCTATGAAA CTAGCCCAGC TCCCAGTACA 
AGTTTCTGGA GCTCGGTGCC AGTGATGAGT CCATCCTTTG CCTCCCCCCC TGCCCGTGTG 
GCTGGCCAGA TGGTTGTGCC CTCCATTTCC CTCAGCCCGC ATGGTGGCAT GGCCTCTGGA 
ACTGCCACAG GAAGCCAGCC CTCCTCTCCA CGGTATCGCC CCTACACTGT CACCCACCCG 
TGGGGGTCTT CGACTTCTCC TACCCCGCGA TCCCCAGGCA TCTCCATTCT GTCCAGCTCG 
CCCAGCCTCC CTGCCCTTGC TAGTAGCCCT CAAGCGGTTC CTGCCAGCTC TCTCAGACAG 
AGACCCAGCA GCCTGGGGCC CCCTTCAGTG GCCGCCTCAC CCAGTGCCGT GAGCAGACGT 
CCCTCATCCC TAAGGATCTC TCCTAGTTTG GGAGCCTCTG GTGGGGCAAG TACTTGGGAT 
TCCTACAGCG ACCATTTCAC CATTGAGACC TGCAAGGAGA CAGACATGCT GAACTACCTC 
ATCGAGTGTT TTGACCGAGT GGGAATAGAG GAAAAAAAAG CACCAAAGAT GTGCAGCCAG 
CCAGCAGTCA GCCAGCTTCT CAGCAACATC CGCTCACAGT GCATATCCCA TACTGCGTTA 
GTCCTACAAG GTTCCCTCAC ACAACCAAGG TCCATGCAGC AGCCATCCTT CCTGGTGCCG 
TATATGCTCT GCAGGAATCT CCCATATGGC TTCATGCAGG AACTGGTGAG AACCACTCAC 
CAGGATGAAG AAGTGTTCAA GCAGATCTTT ATCCCCATTT TACAAGGCCT GGCTCTCGCT 
GCCAAAGAGT GCTCCCTCGA TAGTGACTAC TTTAAGTACC CCCTCATGGC TTTAGGTGAG 
CTTTGTGAAA CCAAGTTTGG GAAGACACAC CCCGTGTGCA ATTTGGTGGC TTCTTTGCCC 
TTGTGGTTGC CGAAATCCTT AAGCCCTGGC TCTGGGCGGG AGCTGCAGAG ACTCTCTTAT 
TTGGGAGCTT TCTTTAGCTT CTCAGTCTTT GCGGAAGATG ACGCTAAAGT GGTTGAAAAA 
TATTTCTCAG GGCCTGCCAT TACCCTGGAA AACACTCGCG TGGTCAGCCA ATCACTGCAG 
CATTACTTGG AGCTGGGAAG GCAAGAGCTT TTCAAGATTC TGCATAGTAT TCTGTTAAAC 
GGTGAGACCC GTGAGGCCGC TCTTGGTTAC ATGGCAGCTG TCGTCAATGC CAATATGAAG 
AAAGCACAGA TGCAGACAGA TGATAGATTG GTATCTACAG ATGGATTCAT GCTGAATTTC 
CTTTGGGTGC TGCAGCAGCT AAGTACAAAG ATCAAGTTAG AGACGGTGGA TCCCACGTAT 
ATATTCCACC CAAGGTGTCG GATTACTCTT CCTAATGACG AGACGAGAGT GAATGCTACA 
ATGGAGGATG TGAATGACTG GCTGGCTGAA CTTTATGGAG ACCAGCCTCC ATTTTCTGAG 
CCGAAATTTC CTACAGAATG CTTTTTTCTT ACCCTGCATG CCCACCACCT CTCCATTCTG 
CCAAGCTGCC GTCGCTATAT CCGTAGACTG CGGGCCATCC GGGAGCTCAA TAGAACTGTG 
GAAGATTTGA AAAATAATGA AAGTCAATGG AAAGATTCCC CACTGGCAAC CAGACACCGT 
GAAATGCTGA AGCGCTGCAA AACTCAGCTT AAGAAATTGG TGCGGTGCAA GGCCTGTGCT 
GATGCTGGTT TACTTGATGA GAGCTTTCTG AGAAGATGTC TGAATTTTTA TGGCCTTCTC 
ATCCAGCTGC TGCTCCGCAT CCTGGACCCC GCCTATCCCG ATATAACACT GCCTTTAAAC 
TCTGATGTCC CCAAGGTATT TGCAGCATTG CCTGAGTTTT ATGTAGAAGA TGTTGCTGAA 
TTTTTATTTT TTATTGTACA ATACTCTCCT CAGGTGCTGT ATGAGCCCTG TACTCAGGAT 
ATTGTGATGT TCCTCGTTGT GATGTTGTGT AACCAGAACT ACATCCGAAA TCCATATCTG 
GTGGCCAAAC TGGTAGAAGT CATGTTTATG ACCAACCCAG CTGTTCAGCC ACGAACCCAG 
AAGTTTTTTG AAATGATTGA AAACCATCCT CTCTCCACCA AGTTGTTGGT CCCTTCACTG 
ATGAAGTTTT ATACAGATGT TGAACACACC GGAGCCACCA GTGAGTTCTA CGACAAGTTC 
ACTATTCGCT ATCACATTAG CACCATTTTT AAAAGCCTTT GGCAAAACAT AGCTCACCAT 
GGCACCTTCA TGGAGGAGTT CAATTCGGGG AAGCAGTTTG TTCGCTATAT AAACATGCTG 
ATAAATGACA CGACCTTTTT ACTCGATGAA AGCCTGGAGT CTCTGAAGCG AATCCACGAA 
GTACAAGAAG AAATGAAGAA CAAAGAACAG TGGGACCAGC TGCCCCGGGA TCAGCAGCAA 
GCCCGGCAGT CCCAGCTTGC TCAGGACGAG CGGGTTTCAC GCTCCTACCT TGCCCTGGCA 
ACAGAGACCG TGGACATGTT CCATATCCTC ACGAAACAGG TCCAGAAGCC CTTCCTCAGA 
CCGGAACTTG GACCCCGATT GGCTGCAATG CTGAACTTTA ATCTCCAGCA ACTCTGTGGC 
CCCAAGTGCC GCGACCTGAA AGTTGAAAAC CCTGAGAAAT ATGGCTTTGA ACCAAAGAAG 
CTGTTGGACC AACTGACAGA CATTTACCTG CAGCTGGACT GTGCCCGGTT TGCCAAAGCC 
ATCGCTGATG ACCAGAGATC CTACAGCAAA GAGTTGTTTG AAGAAGTGAT CTCGAAGATG 
CGGAAGGCAG GGATCAAATC CACAATAGCT ATCGAGAAGT TTAAACTCCT CGCTGAGAAG 
GTGGAGGAGA TAGTGGCCAA GAACGCACGC GCAGAAATCG ACTACAGCGA CGCCCCCGAC 
GAGTTCAGAG ACCCACTGAT GGACACCCTG ATGACTGACC CTGTGCGGCT GCCCTCTGGC 
ACCATCATGG ACCGCTCCAT CATCCTGCGG CACTTGCTGA ACTCCCCCAC TGACCCCTTC 
AATCGACAGA TGCTGACAGA GAACATGCTG GAACCAGTGC CAGAACTGAA AGAACAGATT 
CACGCCTGGA TGAGAGAGAA ACAAAATAGT GATCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022274748.1
CDS	717..4655
Translation	MEELSADEIRRRRLARLAGGQTSQPTTPLTSPQRENPLGPPIAASAPGPSQSLGLNVHNMTPATSPIGASGVAHRSQSSEGVSSLSSSPSNSLETQSQSLSRSQSMDIDGVSCEKSMSQVDVDSGIENMEVDENDRREKRSLSDKEPSSGSEVSEEQALQLVCKIFRVSWKDRDRDVIFLSSLSAQFKQNPKEVFSDFKDLIGQILMEVLMMSTQTRDENPFASLTATSQPIAAAARSPDRNLMLTTGSNPGTSPMFCGVGSFGASSFSSLYETSPAPSTSFWSSVPVMSPSFASPPARVAGQMVVPSISLSPHGGMASGTATGSQPSSPRYRPYTVTHPWGSSTSPTPRSPGISILSSSPSLPALASSPQAVPASSLRQRPSSLGPPSVAASPSAVSRRPSSLRISPSLGASGGASTWDSYSDHFTIETCKETDMLNYLIECFDRVGIEEKKAPKMCSQPAVSQLLSNIRSQCISHTALVLQGSLTQPRSMQQPSFLVPYMLCRNLPYGFMQELVRTTHQDEEVFKQIFIPILQGLALAAKECSLDSDYFKYPLMALGELCETKFGKTHPVCNLVASLPLWLPKSLSPGSGRELQRLSYLGAFFSFSVFAEDDAKVVEKYFSGPAITLENTRVVSQSLQHYLELGRQELFKILHSILLNGETREAALGYMAAVVNANMKKAQMQTDDRLVSTDGFMLNFLWVLQQLSTKIKLETVDPTYIFHPRCRITLPNDETRVNATMEDVNDWLAELYGDQPPFSEPKFPTECFFLTLHAHHLSILPSCRRYIRRLRAIRELNRTVEDLKNNESQWKDSPLATRHREMLKRCKTQLKKLVRCKACADAGLLDESFLRRCLNFYGLLIQLLLRILDPAYPDITLPLNSDVPKVFAALPEFYVEDVAEFLFFIVQYSPQVLYEPCTQDIVMFLVVMLCNQNYIRNPYLVAKLVEVMFMTNPAVQPRTQKFFEMIENHPLSTKLLVPSLMKFYTDVEHTGATSEFYDKFTIRYHISTIFKSLWQNIAHHGTFMEEFNSGKQFVRYINMLINDTTFLLDESLESLKRIHEVQEEMKNKEQWDQLPRDQQQARQSQLAQDERVSRSYLALATETVDMFHILTKQVQKPFLRPELGPRLAAMLNFNLQQLCGPKCRDLKVENPEKYGFEPKKLLDQLTDIYLQLDCARFAKAIADDQRSYSKELFEEVISKMRKAGIKSTIAIEKFKLLAEKVEEIVAKNARAEIDYSDAPDEFRDPLMDTLMTDPVRLPSGTIMDRSIILRHLLNSPTDPFNRQMLTENMLEPVPELKEQIHAWMREKQNSDH

Target ORF information:

RefSeq Version	XM_022419040.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ubiquitination factor E4B (UBE4B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022419040.1

ORF Insert Sequence:

ATGGAGGAGC TGAGCGCTGA TGAGATCCGA CGGAGGCGCC TTGCACGACT TGCTGGTGGA 
CAGACCTCCC AGCCAACCAC CCCACTCACC TCGCCCCAGA GGGAGAACCC TCTGGGGCCT 
CCTATCGCAG CATCAGCCCC AGGCCCTTCC CAGAGCCTTG GTCTCAATGT CCACAACATG 
ACCCCAGCTA CCTCCCCTAT AGGTGCATCA GGAGTAGCTC ACCGAAGCCA GAGCAGTGAA 
GGAGTCAGTT CTCTCAGCAG CTCGCCCTCC AATAGCCTTG AAACCCAATC TCAGTCTCTC 
TCACGTTCCC AGAGCATGGA TATCGATGGG GTCTCTTGTG AAAAAAGCAT GTCCCAGGTG 
GATGTGGATT CAGGAATAGA AAATATGGAG GTTGATGAAA ACGATCGAAG AGAAAAGAGG 
AGCCTCAGCG ATAAGGAGCC TTCCTCGGGT TCTGAAGTAT CTGAAGAACA GGCCTTACAG 
CTGGTCTGTA AGATCTTTCG TGTCTCCTGG AAGGACCGGG ACAGAGATGT CATCTTTCTT 
TCTTCTCTTT CTGCACAATT TAAGCAGAAC CCAAAAGAAG TGTTCTCTGA TTTTAAGGAC 
TTGATTGGCC AGATTTTAAT GGAAGTGCTA ATGATGTCTA CGCAGACTAG AGATGAAAAT 
CCATTTGCCA GTCTGACAGC CACATCACAG CCAATTGCAG CAGCAGCTCG GTCACCAGAC 
AGGAATCTAA TGCTCACTAC TGGCTCTAAC CCAGGAACAA GCCCCATGTT CTGTGGTGTG 
GGTTCCTTTG GTGCCAGCTC TTTCTCTAGC CTCTATGAAA CTAGCCCAGC TCCCAGTACA 
AGTTTCTGGA GCTCGGTGCC AGTGATGAGT CCATCCTTTG CCTCCCCCCC TGCCCGTGTG 
GCTGGCCAGA TGGTTGTGCC CTCCATTTCC CTCAGCCCGC ATGGTGGCAT GGCCTCTGGA 
ACTGCCACAG GAAGCCAGCC CTCCTCTCCA CGGTATCGCC CCTACACTGT CACCCACCCG 
TGGGGGTCTT CGACTTCTCC TACCCCGCGA TCCCCAGGCA TCTCCATTCT GTCCAGCTCG 
CCCAGCCTCC CTGCCCTTGC TAGTAGCCCT CAAGCGGTTC CTGCCAGCTC TCTCAGACAG 
AGACCCAGCA GCCTGGGGCC CCCTTCAGTG GCCGCCTCAC CCAGTGCCGT GAGCAGACGT 
CCCTCATCCC TAAGGATCTC TCCTAGTTTG GGAGCCTCTG GTGGGGCAAG TACTTGGGAT 
TCCTACAGCG ACCATTTCAC CATTGAGACC TGCAAGGAGA CAGACATGCT GAACTACCTC 
ATCGAGTGTT TTGACCGAGT GGGAATAGAG GAAAAAAAAG CACCAAAGAT GTGCAGCCAG 
CCAGCAGTCA GCCAGCTTCT CAGCAACATC CGCTCACAGT GCATATCCCA TACTGCGTTA 
GTCCTACAAG GTTCCCTCAC ACAACCAAGG TCCATGCAGC AGCCATCCTT CCTGGTGCCG 
TATATGCTCT GCAGGAATCT CCCATATGGC TTCATGCAGG AACTGGTGAG AACCACTCAC 
CAGGATGAAG AAGTGTTCAA GCAGATCTTT ATCCCCATTT TACAAGGCCT GGCTCTCGCT 
GCCAAAGAGT GCTCCCTCGA TAGTGACTAC TTTAAGTACC CCCTCATGGC TTTAGGTGAG 
CTTTGTGAAA CCAAGTTTGG GAAGACACAC CCCGTGTGCA ATTTGGTGGC TTCTTTGCCC 
TTGTGGTTGC CGAAATCCTT AAGCCCTGGC TCTGGGCGGG AGCTGCAGAG ACTCTCTTAT 
TTGGGAGCTT TCTTTAGCTT CTCAGTCTTT GCGGAAGATG ACGCTAAAGT GGTTGAAAAA 
TATTTCTCAG GGCCTGCCAT TACCCTGGAA AACACTCGCG TGGTCAGCCA ATCACTGCAG 
CATTACTTGG AGCTGGGAAG GCAAGAGCTT TTCAAGATTC TGCATAGTAT TCTGTTAAAC 
GGTGAGACCC GTGAGGCCGC TCTTGGTTAC ATGGCAGCTG TCGTCAATGC CAATATGAAG 
AAAGCACAGA TGCAGACAGA TGATAGATTG GTATCTACAG ATGGATTCAT GCTGAATTTC 
CTTTGGGTGC TGCAGCAGCT AAGTACAAAG ATCAAGTTAG AGACGGTGGA TCCCACGTAT 
ATATTCCACC CAAGGTGTCG GATTACTCTT CCTAATGACG AGACGAGAGT GAATGCTACA 
ATGGAGGATG TGAATGACTG GCTGGCTGAA CTTTATGGAG ACCAGCCTCC ATTTTCTGAG 
CCGAAATTTC CTACAGAATG CTTTTTTCTT ACCCTGCATG CCCACCACCT CTCCATTCTG 
CCAAGCTGCC GTCGCTATAT CCGTAGACTG CGGGCCATCC GGGAGCTCAA TAGAACTGTG 
GAAGATTTGA AAAATAATGA AAGTCAATGG AAAGATTCCC CACTGGCAAC CAGACACCGT 
GAAATGCTGA AGCGCTGCAA AACTCAGCTT AAGAAATTGG TGCGGTGCAA GGCCTGTGCT 
GATGCTGGTT TACTTGATGA GAGCTTTCTG AGAAGATGTC TGAATTTTTA TGGCCTTCTC 
ATCCAGCTGC TGCTCCGCAT CCTGGACCCC GCCTATCCCG ATATAACACT GCCTTTAAAC 
TCTGATGTCC CCAAGGTATT TGCAGCATTG CCTGAGTTTT ATGTAGAAGA TGTTGCTGAA 
TTTTTATTTT TTATTGTACA ATACTCTCCT CAGGTGCTGT ATGAGCCCTG TACTCAGGAT 
ATTGTGATGT TCCTCGTTGT GATGTTGTGT AACCAGAACT ACATCCGAAA TCCATATCTG 
GTGGCCAAAC TGGTAGAAGT CATGTTTATG ACCAACCCAG CTGTTCAGCC ACGAACCCAG 
AAGTTTTTTG AAATGATTGA AAACCATCCT CTCTCCACCA AGTTGTTGGT CCCTTCACTG 
ATGAAGTTTT ATACAGATGT TGAACACACC GGAGCCACCA GTGAGTTCTA CGACAAGTTC 
ACTATTCGCT ATCACATTAG CACCATTTTT AAAAGCCTTT GGCAAAACAT AGCTCACCAT 
GGCACCTTCA TGGAGGAGTT CAATTCGGGG AAGCAGTTTG TTCGCTATAT AAACATGCTG 
ATAAATGACA CGACCTTTTT ACTCGATGAA AGCCTGGAGT CTCTGAAGCG AATCCACGAA 
GTACAAGAAG AAATGAAGAA CAAAGAACAG TGGGACCAGC TGCCCCGGGA TCAGCAGCAA 
GCCCGGCAGT CCCAGCTTGC TCAGGACGAG CGGGTTTCAC GCTCCTACCT TGCCCTGGCA 
ACAGAGACCG TGGACATGTT CCATATCCTC ACGAAACAGG TCCAGAAGCC CTTCCTCAGA 
CCGGAACTTG GACCCCGATT GGCTGCAATG CTGAACTTTA ATCTCCAGCA ACTCTGTGGC 
CCCAAGTGCC GCGACCTGAA AGTTGAAAAC CCTGAGAAAT ATGGCTTTGA ACCAAAGAAG 
CTGTTGGACC AACTGACAGA CATTTACCTG CAGCTGGACT GTGCCCGGTT TGCCAAAGCC 
ATCGCTGATG ACCAGAGATC CTACAGCAAA GAGTTGTTTG AAGAAGTGAT CTCGAAGATG 
CGGAAGGCAG GGATCAAATC CACAATAGCT ATCGAGAAGT TTAAACTCCT CGCTGAGAAG 
GTGGAGGAGA TAGTGGCCAA GAACGCACGC GCAGAAATCG ACTACAGCGA CGCCCCCGAC 
GAGTTCAGAG ACCCACTGAT GGACACCCTG ATGACTGACC CTGTGCGGCT GCCCTCTGGC 
ACCATCATGG ACCGCTCCAT CATCCTGCGG CACTTGCTGA ACTCCCCCAC TGACCCCTTC 
AATCGACAGA TGCTGACAGA GAACATGCTG GAACCAGTGC CAGAACTGAA AGAACAGATT 
CACGCCTGGA TGAGAGAGAA ACAAAATAGT GATCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE4B ( XM_005620527.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005620584.1 Canis lupus familiaris ubiquitination factor E4B (UBE4B), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe06877
Clone ID Related Accession (Same CDS sequence)	XM_005620527.3 , XM_005620527.2
Accession Version	XM_005620527.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	ubiquitin conjugation factor E4 B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005620527.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCAGG TGGATGTGGA TTCAGGAATA GAAAATATGG AGGTTGATGA AAACGATCGA 
AGAGAAAAGA GGAGCCTCAG CGATAAGGAG CCTTCCTCGG GTTCTGAAGT ATCTGAAGAA 
CAGGCCTTAC AGCTGGTCTG TAAGATCTTT CGTGTCTCCT GGAAGGACCG GGACAGAGAT 
GTCATCTTTC TTTCTTCTCT TTCTGCACAA TTTAAGCAGA ACCCAAAAGA AGTGTTCTCT 
GATTTTAAGG ACTTGATTGG CCAGATTTTA ATGGAAGTGC TAATGATGTC TACGCAGACT 
AGAGATGAAA ATCCATTTGC CAGTCTGACA GCCACATCAC AGCCAATTGC AGCAGCAGCT 
CGGTCACCAG ACAGGAATCT AATGCTCACT ACTGGCTCTA ACCCAGGAAC AAGCCCCATG 
TTCTGTGGTG TGGGTTCCTT TGGTGCCAGC TCTTTCTCTA GCCTCTATGA AACTAGCCCA 
GCTCCCAGTA CAAGTTTCTG GAGCTCGGTG CCAGTGATGA GTCCATCCTT TGCCTCCCCC 
CCTGCCCGTG TGGCTGGCCA GATGGTTGTG CCCTCCATTT CCCTCAGCCC GCATGGTGGC 
ATGGCCTCTG GAACTGCCAC AGGAAGCCAG CCCTCCTCTC CACGGTATCG CCCCTACACT 
GTCACCCACC CGTGGGGGTC TTCGACTTCT CCTACCCCGC GATCCCCAGG CATCTCCATT 
CTGTCCAGCT CGCCCAGCCT CCCTGCCCTT GCTAGTAGCC CTCAAGCGGT TCCTGCCAGC 
TCTCTCAGAC AGAGACCCAG CAGCCTGGGG CCCCCTTCAG TGGCCGCCTC ACCCAGTGCC 
GTGAGCAGAC GTCCCTCATC CCTAAGGATC TCTCCTAGTA TGTACGACAA TCCTTTCTCC 
TTCCTCTTCC TCGCACTTTC TGGCGACAGT AGTGATGAAG ATGAAGAAGA TGATGATGAT 
GATGATGATG GTGATGATGA AGGTGGTGGT GGTGGTGATG AGTTTTCTTG TGTCCAGTTT 
GGGTCCAGTT TGGGAGCCTC TGGTGGGGCA AGTACTTGGG ATTCCTACAG CGACCATTTC 
ACCATTGAGA CCTGCAAGGA GACAGACATG CTGAACTACC TCATCGAGTG TTTTGACCGA 
GTGGGAATAG AGGAAAAAAA AGCACCAAAG ATGTGCAGCC AGCCAGCAGT CAGCCAGCTT 
CTCAGCAACA TCCGCTCACA GTGCATATCC CATACTGCGT TAGTCCTACA AGGTTCCCTC 
ACACAACCAA GGTCCATGCA GCAGCCATCC TTCCTGGTGC CGTATATGCT CTGCAGGAAT 
CTCCCATATG GCTTCATGCA GGAACTGGTG AGAACCACTC ACCAGGATGA AGAAGTGTTC 
AAGCAGATCT TTATCCCCAT TTTACAAGGC CTGGCTCTCG CTGCCAAAGA GTGCTCCCTC 
GATAGTGACT ACTTTAAGTA CCCCCTCATG GCTTTAGGTG AGCTTTGTGA AACCAAGTTT 
GGGAAGACAC ACCCCGTGTG CAATTTGGTG GCTTCTTTGC CCTTGTGGTT GCCGAAATCC 
TTAAGCCCTG GCTCTGGGCG GGAGCTGCAG AGACTCTCTT ATTTGGGAGC TTTCTTTAGC 
TTCTCAGTCT TTGCGGAAGA TGACGCTAAA GTGGTTGAAA AATATTTCTC AGGGCCTGCC 
ATTACCCTGG AAAACACTCG CGTGGTCAGC CAATCACTGC AGCATTACTT GGAGCTGGGA 
AGGCAAGAGC TTTTCAAGAT TCTGCATAGT ATTCTGTTAA ACGGTGAGAC CCGTGAGGCC 
GCTCTTGGTT ACATGGCAGC TGTCGTCAAT GCCAATATGA AGAAAGCACA GATGCAGACA 
GATGATAGAT TGGTATCTAC AGATGGATTC ATGCTGAATT TCCTTTGGGT GCTGCAGCAG 
CTAAGTACAA AGATCAAGTT AGAGACGGTG GATCCCACGT ATATATTCCA CCCAAGGTGT 
CGGATTACTC TTCCTAATGA CGAGACGAGA GTGAATGCTA CAATGGAGGA TGTGAATGAC 
TGGCTGGCTG AACTTTATGG AGACCAGCCT CCATTTTCTG AGCCGAAATT TCCTACAGAA 
TGCTTTTTTC TTACCCTGCA TGCCCACCAC CTCTCCATTC TGCCAAGCTG CCGTCGCTAT 
ATCCGTAGAC TGCGGGCCAT CCGGGAGCTC AATAGAACTG TGGAAGATTT GAAAAATAAT 
GAAAGTCAAT GGAAAGATTC CCCACTGGCA ACCAGACACC GTGAAATGCT GAAGCGCTGC 
AAAACTCAGC TTAAGAAATT GGTGCGGTGC AAGGCCTGTG CTGATGCTGG TTTACTTGAT 
GAGAGCTTTC TGAGAAGATG TCTGAATTTT TATGGCCTTC TCATCCAGCT GCTGCTCCGC 
ATCCTGGACC CCGCCTATCC CGATATAACA CTGCCTTTAA ACTCTGATGT CCCCAAGGTA 
TTTGCAGCAT TGCCTGAGTT TTATGTAGAA GATGTTGCTG AATTTTTATT TTTTATTGTA 
CAATACTCTC CTCAGGTGCT GTATGAGCCC TGTACTCAGG ATATTGTGAT GTTCCTCGTT 
GTGATGTTGT GTAACCAGAA CTACATCCGA AATCCATATC TGGTGGCCAA ACTGGTAGAA 
GTCATGTTTA TGACCAACCC AGCTGTTCAG CCACGAACCC AGAAGTTTTT TGAAATGATT 
GAAAACCATC CTCTCTCCAC CAAGTTGTTG GTCCCTTCAC TGATGAAGTT TTATACAGAT 
GTTGAACACA CCGGAGCCAC CAGTGAGTTC TACGACAAGT TCACTATTCG CTATCACATT 
AGCACCATTT TTAAAAGCCT TTGGCAAAAC ATAGCTCACC ATGGCACCTT CATGGAGGAG 
TTCAATTCGG GGAAGCAGTT TGTTCGCTAT ATAAACATGC TGATAAATGA CACGACCTTT 
TTACTCGATG AAAGCCTGGA GTCTCTGAAG CGAATCCACG AAGTACAAGA AGAAATGAAG 
AACAAAGAAC AGTGGGACCA GCTGCCCCGG GATCAGCAGC AAGCCCGGCA GTCCCAGCTT 
GCTCAGGACG AGCGGGTTTC ACGCTCCTAC CTTGCCCTGG CAACAGAGAC CGTGGACATG 
TTCCATATCC TCACGAAACA GGTCCAGAAG CCCTTCCTCA GACCGGAACT TGGACCCCGA 
TTGGCTGCAA TGCTGAACTT TAATCTCCAG CAACTCTGTG GCCCCAAGTG CCGCGACCTG 
AAAGTTGAAA ACCCTGAGAA ATATGGCTTT GAACCAAAGA AGCTGTTGGA CCAACTGACA 
GACATTTACC TGCAGCTGGA CTGTGCCCGG TTTGCCAAAG CCATCGCTGA TGACCAGAGA 
TCCTACAGCA AAGAGTTGTT TGAAGAAGTG ATCTCGAAGA TGCGGAAGGC AGGGATCAAA 
TCCACAATAG CTATCGAGAA GTTTAAACTC CTCGCTGAGA AGGTGGAGGA GATAGTGGCC 
AAGAACGCAC GCGCAGAAAT CGACTACAGC GACGCCCCCG ACGAGTTCAG AGACCCACTG 
ATGGACACCC TGATGACTGA CCCTGTGCGG CTGCCCTCTG GCACCATCAT GGACCGCTCC 
ATCATCCTGC GGCACTTGCT GAACTCCCCC ACTGACCCCT TCAATCGACA GATGCTGACA 
GAGAACATGC TGGAACCAGT GCCAGAACTG AAAGAACAGA TTCACGCCTG GATGAGAGAG 
AAACAAAATA GTGATCATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620584.1
CDS	217..3957
Translation	MSQVDVDSGIENMEVDENDRREKRSLSDKEPSSGSEVSEEQALQLVCKIFRVSWKDRDRDVIFLSSLSAQFKQNPKEVFSDFKDLIGQILMEVLMMSTQTRDENPFASLTATSQPIAAAARSPDRNLMLTTGSNPGTSPMFCGVGSFGASSFSSLYETSPAPSTSFWSSVPVMSPSFASPPARVAGQMVVPSISLSPHGGMASGTATGSQPSSPRYRPYTVTHPWGSSTSPTPRSPGISILSSSPSLPALASSPQAVPASSLRQRPSSLGPPSVAASPSAVSRRPSSLRISPSMYDNPFSFLFLALSGDSSDEDEEDDDDDDDGDDEGGGGGDEFSCVQFGSSLGASGGASTWDSYSDHFTIETCKETDMLNYLIECFDRVGIEEKKAPKMCSQPAVSQLLSNIRSQCISHTALVLQGSLTQPRSMQQPSFLVPYMLCRNLPYGFMQELVRTTHQDEEVFKQIFIPILQGLALAAKECSLDSDYFKYPLMALGELCETKFGKTHPVCNLVASLPLWLPKSLSPGSGRELQRLSYLGAFFSFSVFAEDDAKVVEKYFSGPAITLENTRVVSQSLQHYLELGRQELFKILHSILLNGETREAALGYMAAVVNANMKKAQMQTDDRLVSTDGFMLNFLWVLQQLSTKIKLETVDPTYIFHPRCRITLPNDETRVNATMEDVNDWLAELYGDQPPFSEPKFPTECFFLTLHAHHLSILPSCRRYIRRLRAIRELNRTVEDLKNNESQWKDSPLATRHREMLKRCKTQLKKLVRCKACADAGLLDESFLRRCLNFYGLLIQLLLRILDPAYPDITLPLNSDVPKVFAALPEFYVEDVAEFLFFIVQYSPQVLYEPCTQDIVMFLVVMLCNQNYIRNPYLVAKLVEVMFMTNPAVQPRTQKFFEMIENHPLSTKLLVPSLMKFYTDVEHTGATSEFYDKFTIRYHISTIFKSLWQNIAHHGTFMEEFNSGKQFVRYINMLINDTTFLLDESLESLKRIHEVQEEMKNKEQWDQLPRDQQQARQSQLAQDERVSRSYLALATETVDMFHILTKQVQKPFLRPELGPRLAAMLNFNLQQLCGPKCRDLKVENPEKYGFEPKKLLDQLTDIYLQLDCARFAKAIADDQRSYSKELFEEVISKMRKAGIKSTIAIEKFKLLAEKVEEIVAKNARAEIDYSDAPDEFRDPLMDTLMTDPVRLPSGTIMDRSIILRHLLNSPTDPFNRQMLTENMLEPVPELKEQIHAWMREKQNSDH

Target ORF information:

RefSeq Version	XM_005620527.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ubiquitination factor E4B (UBE4B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005620527.3

ORF Insert Sequence:

ATGTCCCAGG TGGATGTGGA TTCAGGAATA GAAAATATGG AGGTTGATGA AAACGATCGA 
AGAGAAAAGA GGAGCCTCAG CGATAAGGAG CCTTCCTCGG GTTCTGAAGT ATCTGAAGAA 
CAGGCCTTAC AGCTGGTCTG TAAGATCTTT CGTGTCTCCT GGAAGGACCG GGACAGAGAT 
GTCATCTTTC TTTCTTCTCT TTCTGCACAA TTTAAGCAGA ACCCAAAAGA AGTGTTCTCT 
GATTTTAAGG ACTTGATTGG CCAGATTTTA ATGGAAGTGC TAATGATGTC TACGCAGACT 
AGAGATGAAA ATCCATTTGC CAGTCTGACA GCCACATCAC AGCCAATTGC AGCAGCAGCT 
CGGTCACCAG ACAGGAATCT AATGCTCACT ACTGGCTCTA ACCCAGGAAC AAGCCCCATG 
TTCTGTGGTG TGGGTTCCTT TGGTGCCAGC TCTTTCTCTA GCCTCTATGA AACTAGCCCA 
GCTCCCAGTA CAAGTTTCTG GAGCTCGGTG CCAGTGATGA GTCCATCCTT TGCCTCCCCC 
CCTGCCCGTG TGGCTGGCCA GATGGTTGTG CCCTCCATTT CCCTCAGCCC GCATGGTGGC 
ATGGCCTCTG GAACTGCCAC AGGAAGCCAG CCCTCCTCTC CACGGTATCG CCCCTACACT 
GTCACCCACC CGTGGGGGTC TTCGACTTCT CCTACCCCGC GATCCCCAGG CATCTCCATT 
CTGTCCAGCT CGCCCAGCCT CCCTGCCCTT GCTAGTAGCC CTCAAGCGGT TCCTGCCAGC 
TCTCTCAGAC AGAGACCCAG CAGCCTGGGG CCCCCTTCAG TGGCCGCCTC ACCCAGTGCC 
GTGAGCAGAC GTCCCTCATC CCTAAGGATC TCTCCTAGTA TGTACGACAA TCCTTTCTCC 
TTCCTCTTCC TCGCACTTTC TGGCGACAGT AGTGATGAAG ATGAAGAAGA TGATGATGAT 
GATGATGATG GTGATGATGA AGGTGGTGGT GGTGGTGATG AGTTTTCTTG TGTCCAGTTT 
GGGTCCAGTT TGGGAGCCTC TGGTGGGGCA AGTACTTGGG ATTCCTACAG CGACCATTTC 
ACCATTGAGA CCTGCAAGGA GACAGACATG CTGAACTACC TCATCGAGTG TTTTGACCGA 
GTGGGAATAG AGGAAAAAAA AGCACCAAAG ATGTGCAGCC AGCCAGCAGT CAGCCAGCTT 
CTCAGCAACA TCCGCTCACA GTGCATATCC CATACTGCGT TAGTCCTACA AGGTTCCCTC 
ACACAACCAA GGTCCATGCA GCAGCCATCC TTCCTGGTGC CGTATATGCT CTGCAGGAAT 
CTCCCATATG GCTTCATGCA GGAACTGGTG AGAACCACTC ACCAGGATGA AGAAGTGTTC 
AAGCAGATCT TTATCCCCAT TTTACAAGGC CTGGCTCTCG CTGCCAAAGA GTGCTCCCTC 
GATAGTGACT ACTTTAAGTA CCCCCTCATG GCTTTAGGTG AGCTTTGTGA AACCAAGTTT 
GGGAAGACAC ACCCCGTGTG CAATTTGGTG GCTTCTTTGC CCTTGTGGTT GCCGAAATCC 
TTAAGCCCTG GCTCTGGGCG GGAGCTGCAG AGACTCTCTT ATTTGGGAGC TTTCTTTAGC 
TTCTCAGTCT TTGCGGAAGA TGACGCTAAA GTGGTTGAAA AATATTTCTC AGGGCCTGCC 
ATTACCCTGG AAAACACTCG CGTGGTCAGC CAATCACTGC AGCATTACTT GGAGCTGGGA 
AGGCAAGAGC TTTTCAAGAT TCTGCATAGT ATTCTGTTAA ACGGTGAGAC CCGTGAGGCC 
GCTCTTGGTT ACATGGCAGC TGTCGTCAAT GCCAATATGA AGAAAGCACA GATGCAGACA 
GATGATAGAT TGGTATCTAC AGATGGATTC ATGCTGAATT TCCTTTGGGT GCTGCAGCAG 
CTAAGTACAA AGATCAAGTT AGAGACGGTG GATCCCACGT ATATATTCCA CCCAAGGTGT 
CGGATTACTC TTCCTAATGA CGAGACGAGA GTGAATGCTA CAATGGAGGA TGTGAATGAC 
TGGCTGGCTG AACTTTATGG AGACCAGCCT CCATTTTCTG AGCCGAAATT TCCTACAGAA 
TGCTTTTTTC TTACCCTGCA TGCCCACCAC CTCTCCATTC TGCCAAGCTG CCGTCGCTAT 
ATCCGTAGAC TGCGGGCCAT CCGGGAGCTC AATAGAACTG TGGAAGATTT GAAAAATAAT 
GAAAGTCAAT GGAAAGATTC CCCACTGGCA ACCAGACACC GTGAAATGCT GAAGCGCTGC 
AAAACTCAGC TTAAGAAATT GGTGCGGTGC AAGGCCTGTG CTGATGCTGG TTTACTTGAT 
GAGAGCTTTC TGAGAAGATG TCTGAATTTT TATGGCCTTC TCATCCAGCT GCTGCTCCGC 
ATCCTGGACC CCGCCTATCC CGATATAACA CTGCCTTTAA ACTCTGATGT CCCCAAGGTA 
TTTGCAGCAT TGCCTGAGTT TTATGTAGAA GATGTTGCTG AATTTTTATT TTTTATTGTA 
CAATACTCTC CTCAGGTGCT GTATGAGCCC TGTACTCAGG ATATTGTGAT GTTCCTCGTT 
GTGATGTTGT GTAACCAGAA CTACATCCGA AATCCATATC TGGTGGCCAA ACTGGTAGAA 
GTCATGTTTA TGACCAACCC AGCTGTTCAG CCACGAACCC AGAAGTTTTT TGAAATGATT 
GAAAACCATC CTCTCTCCAC CAAGTTGTTG GTCCCTTCAC TGATGAAGTT TTATACAGAT 
GTTGAACACA CCGGAGCCAC CAGTGAGTTC TACGACAAGT TCACTATTCG CTATCACATT 
AGCACCATTT TTAAAAGCCT TTGGCAAAAC ATAGCTCACC ATGGCACCTT CATGGAGGAG 
TTCAATTCGG GGAAGCAGTT TGTTCGCTAT ATAAACATGC TGATAAATGA CACGACCTTT 
TTACTCGATG AAAGCCTGGA GTCTCTGAAG CGAATCCACG AAGTACAAGA AGAAATGAAG 
AACAAAGAAC AGTGGGACCA GCTGCCCCGG GATCAGCAGC AAGCCCGGCA GTCCCAGCTT 
GCTCAGGACG AGCGGGTTTC ACGCTCCTAC CTTGCCCTGG CAACAGAGAC CGTGGACATG 
TTCCATATCC TCACGAAACA GGTCCAGAAG CCCTTCCTCA GACCGGAACT TGGACCCCGA 
TTGGCTGCAA TGCTGAACTT TAATCTCCAG CAACTCTGTG GCCCCAAGTG CCGCGACCTG 
AAAGTTGAAA ACCCTGAGAA ATATGGCTTT GAACCAAAGA AGCTGTTGGA CCAACTGACA 
GACATTTACC TGCAGCTGGA CTGTGCCCGG TTTGCCAAAG CCATCGCTGA TGACCAGAGA 
TCCTACAGCA AAGAGTTGTT TGAAGAAGTG ATCTCGAAGA TGCGGAAGGC AGGGATCAAA 
TCCACAATAG CTATCGAGAA GTTTAAACTC CTCGCTGAGA AGGTGGAGGA GATAGTGGCC 
AAGAACGCAC GCGCAGAAAT CGACTACAGC GACGCCCCCG ACGAGTTCAG AGACCCACTG 
ATGGACACCC TGATGACTGA CCCTGTGCGG CTGCCCTCTG GCACCATCAT GGACCGCTCC 
ATCATCCTGC GGCACTTGCT GAACTCCCCC ACTGACCCCT TCAATCGACA GATGCTGACA 
GAGAACATGC TGGAACCAGT GCCAGAACTG AAAGAACAGA TTCACGCCTG GATGAGAGAG 
AAACAAAATA GTGATCATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE4B ( XM_005620527.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005620584.1 Canis lupus familiaris ubiquitination factor E4B (UBE4B), transcript variant X2, mRNA.

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CloneID	OCe06877
Clone ID Related Accession (Same CDS sequence)	XM_005620527.3 , XM_005620527.2
Accession Version	XM_005620527.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	ubiquitin conjugation factor E4 B isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726059.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005620527.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCAGG TGGATGTGGA TTCAGGAATA GAAAATATGG AGGTTGATGA AAACGATCGA 
AGAGAAAAGA GGAGCCTCAG CGATAAGGAG CCTTCCTCGG GTTCTGAAGT ATCTGAAGAA 
CAGGCCTTAC AGCTGGTCTG TAAGATCTTT CGTGTCTCCT GGAAGGACCG GGACAGAGAT 
GTCATCTTTC TTTCTTCTCT TTCTGCACAA TTTAAGCAGA ACCCAAAAGA AGTGTTCTCT 
GATTTTAAGG ACTTGATTGG CCAGATTTTA ATGGAAGTGC TAATGATGTC TACGCAGACT 
AGAGATGAAA ATCCATTTGC CAGTCTGACA GCCACATCAC AGCCAATTGC AGCAGCAGCT 
CGGTCACCAG ACAGGAATCT AATGCTCACT ACTGGCTCTA ACCCAGGAAC AAGCCCCATG 
TTCTGTGGTG TGGGTTCCTT TGGTGCCAGC TCTTTCTCTA GCCTCTATGA AACTAGCCCA 
GCTCCCAGTA CAAGTTTCTG GAGCTCGGTG CCAGTGATGA GTCCATCCTT TGCCTCCCCC 
CCTGCCCGTG TGGCTGGCCA GATGGTTGTG CCCTCCATTT CCCTCAGCCC GCATGGTGGC 
ATGGCCTCTG GAACTGCCAC AGGAAGCCAG CCCTCCTCTC CACGGTATCG CCCCTACACT 
GTCACCCACC CGTGGGGGTC TTCGACTTCT CCTACCCCGC GATCCCCAGG CATCTCCATT 
CTGTCCAGCT CGCCCAGCCT CCCTGCCCTT GCTAGTAGCC CTCAAGCGGT TCCTGCCAGC 
TCTCTCAGAC AGAGACCCAG CAGCCTGGGG CCCCCTTCAG TGGCCGCCTC ACCCAGTGCC 
GTGAGCAGAC GTCCCTCATC CCTAAGGATC TCTCCTAGTA TGTACGACAA TCCTTTCTCC 
TTCCTCTTCC TCGCACTTTC TGGCGACAGT AGTGATGAAG ATGAAGAAGA TGATGATGAT 
GATGATGATG GTGATGATGA AGGTGGTGGT GGTGGTGATG AGTTTTCTTG TGTCCAGTTT 
GGGTCCAGTT TGGGAGCCTC TGGTGGGGCA AGTACTTGGG ATTCCTACAG CGACCATTTC 
ACCATTGAGA CCTGCAAGGA GACAGACATG CTGAACTACC TCATCGAGTG TTTTGACCGA 
GTGGGAATAG AGGAAAAAAA AGCACCAAAG ATGTGCAGCC AGCCAGCAGT CAGCCAGCTT 
CTCAGCAACA TCCGCTCACA GTGCATATCC CATACTGCGT TAGTCCTACA AGGTTCCCTC 
ACACAACCAA GGTCCATGCA GCAGCCATCC TTCCTGGTGC CGTATATGCT CTGCAGGAAT 
CTCCCATATG GCTTCATGCA GGAACTGGTG AGAACCACTC ACCAGGATGA AGAAGTGTTC 
AAGCAGATCT TTATCCCCAT TTTACAAGGC CTGGCTCTCG CTGCCAAAGA GTGCTCCCTC 
GATAGTGACT ACTTTAAGTA CCCCCTCATG GCTTTAGGTG AGCTTTGTGA AACCAAGTTT 
GGGAAGACAC ACCCCGTGTG CAATTTGGTG GCTTCTTTGC CCTTGTGGTT GCCGAAATCC 
TTAAGCCCTG GCTCTGGGCG GGAGCTGCAG AGACTCTCTT ATTTGGGAGC TTTCTTTAGC 
TTCTCAGTCT TTGCGGAAGA TGACGCTAAA GTGGTTGAAA AATATTTCTC AGGGCCTGCC 
ATTACCCTGG AAAACACTCG CGTGGTCAGC CAATCACTGC AGCATTACTT GGAGCTGGGA 
AGGCAAGAGC TTTTCAAGAT TCTGCATAGT ATTCTGTTAA ACGGTGAGAC CCGTGAGGCC 
GCTCTTGGTT ACATGGCAGC TGTCGTCAAT GCCAATATGA AGAAAGCACA GATGCAGACA 
GATGATAGAT TGGTATCTAC AGATGGATTC ATGCTGAATT TCCTTTGGGT GCTGCAGCAG 
CTAAGTACAA AGATCAAGTT AGAGACGGTG GATCCCACGT ATATATTCCA CCCAAGGTGT 
CGGATTACTC TTCCTAATGA CGAGACGAGA GTGAATGCTA CAATGGAGGA TGTGAATGAC 
TGGCTGGCTG AACTTTATGG AGACCAGCCT CCATTTTCTG AGCCGAAATT TCCTACAGAA 
TGCTTTTTTC TTACCCTGCA TGCCCACCAC CTCTCCATTC TGCCAAGCTG CCGTCGCTAT 
ATCCGTAGAC TGCGGGCCAT CCGGGAGCTC AATAGAACTG TGGAAGATTT GAAAAATAAT 
GAAAGTCAAT GGAAAGATTC CCCACTGGCA ACCAGACACC GTGAAATGCT GAAGCGCTGC 
AAAACTCAGC TTAAGAAATT GGTGCGGTGC AAGGCCTGTG CTGATGCTGG TTTACTTGAT 
GAGAGCTTTC TGAGAAGATG TCTGAATTTT TATGGCCTTC TCATCCAGCT GCTGCTCCGC 
ATCCTGGACC CCGCCTATCC CGATATAACA CTGCCTTTAA ACTCTGATGT CCCCAAGGTA 
TTTGCAGCAT TGCCTGAGTT TTATGTAGAA GATGTTGCTG AATTTTTATT TTTTATTGTA 
CAATACTCTC CTCAGGTGCT GTATGAGCCC TGTACTCAGG ATATTGTGAT GTTCCTCGTT 
GTGATGTTGT GTAACCAGAA CTACATCCGA AATCCATATC TGGTGGCCAA ACTGGTAGAA 
GTCATGTTTA TGACCAACCC AGCTGTTCAG CCACGAACCC AGAAGTTTTT TGAAATGATT 
GAAAACCATC CTCTCTCCAC CAAGTTGTTG GTCCCTTCAC TGATGAAGTT TTATACAGAT 
GTTGAACACA CCGGAGCCAC CAGTGAGTTC TACGACAAGT TCACTATTCG CTATCACATT 
AGCACCATTT TTAAAAGCCT TTGGCAAAAC ATAGCTCACC ATGGCACCTT CATGGAGGAG 
TTCAATTCGG GGAAGCAGTT TGTTCGCTAT ATAAACATGC TGATAAATGA CACGACCTTT 
TTACTCGATG AAAGCCTGGA GTCTCTGAAG CGAATCCACG AAGTACAAGA AGAAATGAAG 
AACAAAGAAC AGTGGGACCA GCTGCCCCGG GATCAGCAGC AAGCCCGGCA GTCCCAGCTT 
GCTCAGGACG AGCGGGTTTC ACGCTCCTAC CTTGCCCTGG CAACAGAGAC CGTGGACATG 
TTCCATATCC TCACGAAACA GGTCCAGAAG CCCTTCCTCA GACCGGAACT TGGACCCCGA 
TTGGCTGCAA TGCTGAACTT TAATCTCCAG CAACTCTGTG GCCCCAAGTG CCGCGACCTG 
AAAGTTGAAA ACCCTGAGAA ATATGGCTTT GAACCAAAGA AGCTGTTGGA CCAACTGACA 
GACATTTACC TGCAGCTGGA CTGTGCCCGG TTTGCCAAAG CCATCGCTGA TGACCAGAGA 
TCCTACAGCA AAGAGTTGTT TGAAGAAGTG ATCTCGAAGA TGCGGAAGGC AGGGATCAAA 
TCCACAATAG CTATCGAGAA GTTTAAACTC CTCGCTGAGA AGGTGGAGGA GATAGTGGCC 
AAGAACGCAC GCGCAGAAAT CGACTACAGC GACGCCCCCG ACGAGTTCAG AGACCCACTG 
ATGGACACCC TGATGACTGA CCCTGTGCGG CTGCCCTCTG GCACCATCAT GGACCGCTCC 
ATCATCCTGC GGCACTTGCT GAACTCCCCC ACTGACCCCT TCAATCGACA GATGCTGACA 
GAGAACATGC TGGAACCAGT GCCAGAACTG AAAGAACAGA TTCACGCCTG GATGAGAGAG 
AAACAAAATA GTGATCATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620584.1
CDS	94..3834
Translation	MSQVDVDSGIENMEVDENDRREKRSLSDKEPSSGSEVSEEQALQLVCKIFRVSWKDRDRDVIFLSSLSAQFKQNPKEVFSDFKDLIGQILMEVLMMSTQTRDENPFASLTATSQPIAAAARSPDRNLMLTTGSNPGTSPMFCGVGSFGASSFSSLYETSPAPSTSFWSSVPVMSPSFASPPARVAGQMVVPSISLSPHGGMASGTATGSQPSSPRYRPYTVTHPWGSSTSPTPRSPGISILSSSPSLPALASSPQAVPASSLRQRPSSLGPPSVAASPSAVSRRPSSLRISPSMYDNPFSFLFLALSGDSSDEDEEDDDDDDDGDDEGGGGGDEFSCVQFGSSLGASGGASTWDSYSDHFTIETCKETDMLNYLIECFDRVGIEEKKAPKMCSQPAVSQLLSNIRSQCISHTALVLQGSLTQPRSMQQPSFLVPYMLCRNLPYGFMQELVRTTHQDEEVFKQIFIPILQGLALAAKECSLDSDYFKYPLMALGELCETKFGKTHPVCNLVASLPLWLPKSLSPGSGRELQRLSYLGAFFSFSVFAEDDAKVVEKYFSGPAITLENTRVVSQSLQHYLELGRQELFKILHSILLNGETREAALGYMAAVVNANMKKAQMQTDDRLVSTDGFMLNFLWVLQQLSTKIKLETVDPTYIFHPRCRITLPNDETRVNATMEDVNDWLAELYGDQPPFSEPKFPTECFFLTLHAHHLSILPSCRRYIRRLRAIRELNRTVEDLKNNESQWKDSPLATRHREMLKRCKTQLKKLVRCKACADAGLLDESFLRRCLNFYGLLIQLLLRILDPAYPDITLPLNSDVPKVFAALPEFYVEDVAEFLFFIVQYSPQVLYEPCTQDIVMFLVVMLCNQNYIRNPYLVAKLVEVMFMTNPAVQPRTQKFFEMIENHPLSTKLLVPSLMKFYTDVEHTGATSEFYDKFTIRYHISTIFKSLWQNIAHHGTFMEEFNSGKQFVRYINMLINDTTFLLDESLESLKRIHEVQEEMKNKEQWDQLPRDQQQARQSQLAQDERVSRSYLALATETVDMFHILTKQVQKPFLRPELGPRLAAMLNFNLQQLCGPKCRDLKVENPEKYGFEPKKLLDQLTDIYLQLDCARFAKAIADDQRSYSKELFEEVISKMRKAGIKSTIAIEKFKLLAEKVEEIVAKNARAEIDYSDAPDEFRDPLMDTLMTDPVRLPSGTIMDRSIILRHLLNSPTDPFNRQMLTENMLEPVPELKEQIHAWMREKQNSDH

Target ORF information:

RefSeq Version	XM_005620527.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ubiquitination factor E4B (UBE4B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005620527.2

ORF Insert Sequence:

ATGTCCCAGG TGGATGTGGA TTCAGGAATA GAAAATATGG AGGTTGATGA AAACGATCGA 
AGAGAAAAGA GGAGCCTCAG CGATAAGGAG CCTTCCTCGG GTTCTGAAGT ATCTGAAGAA 
CAGGCCTTAC AGCTGGTCTG TAAGATCTTT CGTGTCTCCT GGAAGGACCG GGACAGAGAT 
GTCATCTTTC TTTCTTCTCT TTCTGCACAA TTTAAGCAGA ACCCAAAAGA AGTGTTCTCT 
GATTTTAAGG ACTTGATTGG CCAGATTTTA ATGGAAGTGC TAATGATGTC TACGCAGACT 
AGAGATGAAA ATCCATTTGC CAGTCTGACA GCCACATCAC AGCCAATTGC AGCAGCAGCT 
CGGTCACCAG ACAGGAATCT AATGCTCACT ACTGGCTCTA ACCCAGGAAC AAGCCCCATG 
TTCTGTGGTG TGGGTTCCTT TGGTGCCAGC TCTTTCTCTA GCCTCTATGA AACTAGCCCA 
GCTCCCAGTA CAAGTTTCTG GAGCTCGGTG CCAGTGATGA GTCCATCCTT TGCCTCCCCC 
CCTGCCCGTG TGGCTGGCCA GATGGTTGTG CCCTCCATTT CCCTCAGCCC GCATGGTGGC 
ATGGCCTCTG GAACTGCCAC AGGAAGCCAG CCCTCCTCTC CACGGTATCG CCCCTACACT 
GTCACCCACC CGTGGGGGTC TTCGACTTCT CCTACCCCGC GATCCCCAGG CATCTCCATT 
CTGTCCAGCT CGCCCAGCCT CCCTGCCCTT GCTAGTAGCC CTCAAGCGGT TCCTGCCAGC 
TCTCTCAGAC AGAGACCCAG CAGCCTGGGG CCCCCTTCAG TGGCCGCCTC ACCCAGTGCC 
GTGAGCAGAC GTCCCTCATC CCTAAGGATC TCTCCTAGTA TGTACGACAA TCCTTTCTCC 
TTCCTCTTCC TCGCACTTTC TGGCGACAGT AGTGATGAAG ATGAAGAAGA TGATGATGAT 
GATGATGATG GTGATGATGA AGGTGGTGGT GGTGGTGATG AGTTTTCTTG TGTCCAGTTT 
GGGTCCAGTT TGGGAGCCTC TGGTGGGGCA AGTACTTGGG ATTCCTACAG CGACCATTTC 
ACCATTGAGA CCTGCAAGGA GACAGACATG CTGAACTACC TCATCGAGTG TTTTGACCGA 
GTGGGAATAG AGGAAAAAAA AGCACCAAAG ATGTGCAGCC AGCCAGCAGT CAGCCAGCTT 
CTCAGCAACA TCCGCTCACA GTGCATATCC CATACTGCGT TAGTCCTACA AGGTTCCCTC 
ACACAACCAA GGTCCATGCA GCAGCCATCC TTCCTGGTGC CGTATATGCT CTGCAGGAAT 
CTCCCATATG GCTTCATGCA GGAACTGGTG AGAACCACTC ACCAGGATGA AGAAGTGTTC 
AAGCAGATCT TTATCCCCAT TTTACAAGGC CTGGCTCTCG CTGCCAAAGA GTGCTCCCTC 
GATAGTGACT ACTTTAAGTA CCCCCTCATG GCTTTAGGTG AGCTTTGTGA AACCAAGTTT 
GGGAAGACAC ACCCCGTGTG CAATTTGGTG GCTTCTTTGC CCTTGTGGTT GCCGAAATCC 
TTAAGCCCTG GCTCTGGGCG GGAGCTGCAG AGACTCTCTT ATTTGGGAGC TTTCTTTAGC 
TTCTCAGTCT TTGCGGAAGA TGACGCTAAA GTGGTTGAAA AATATTTCTC AGGGCCTGCC 
ATTACCCTGG AAAACACTCG CGTGGTCAGC CAATCACTGC AGCATTACTT GGAGCTGGGA 
AGGCAAGAGC TTTTCAAGAT TCTGCATAGT ATTCTGTTAA ACGGTGAGAC CCGTGAGGCC 
GCTCTTGGTT ACATGGCAGC TGTCGTCAAT GCCAATATGA AGAAAGCACA GATGCAGACA 
GATGATAGAT TGGTATCTAC AGATGGATTC ATGCTGAATT TCCTTTGGGT GCTGCAGCAG 
CTAAGTACAA AGATCAAGTT AGAGACGGTG GATCCCACGT ATATATTCCA CCCAAGGTGT 
CGGATTACTC TTCCTAATGA CGAGACGAGA GTGAATGCTA CAATGGAGGA TGTGAATGAC 
TGGCTGGCTG AACTTTATGG AGACCAGCCT CCATTTTCTG AGCCGAAATT TCCTACAGAA 
TGCTTTTTTC TTACCCTGCA TGCCCACCAC CTCTCCATTC TGCCAAGCTG CCGTCGCTAT 
ATCCGTAGAC TGCGGGCCAT CCGGGAGCTC AATAGAACTG TGGAAGATTT GAAAAATAAT 
GAAAGTCAAT GGAAAGATTC CCCACTGGCA ACCAGACACC GTGAAATGCT GAAGCGCTGC 
AAAACTCAGC TTAAGAAATT GGTGCGGTGC AAGGCCTGTG CTGATGCTGG TTTACTTGAT 
GAGAGCTTTC TGAGAAGATG TCTGAATTTT TATGGCCTTC TCATCCAGCT GCTGCTCCGC 
ATCCTGGACC CCGCCTATCC CGATATAACA CTGCCTTTAA ACTCTGATGT CCCCAAGGTA 
TTTGCAGCAT TGCCTGAGTT TTATGTAGAA GATGTTGCTG AATTTTTATT TTTTATTGTA 
CAATACTCTC CTCAGGTGCT GTATGAGCCC TGTACTCAGG ATATTGTGAT GTTCCTCGTT 
GTGATGTTGT GTAACCAGAA CTACATCCGA AATCCATATC TGGTGGCCAA ACTGGTAGAA 
GTCATGTTTA TGACCAACCC AGCTGTTCAG CCACGAACCC AGAAGTTTTT TGAAATGATT 
GAAAACCATC CTCTCTCCAC CAAGTTGTTG GTCCCTTCAC TGATGAAGTT TTATACAGAT 
GTTGAACACA CCGGAGCCAC CAGTGAGTTC TACGACAAGT TCACTATTCG CTATCACATT 
AGCACCATTT TTAAAAGCCT TTGGCAAAAC ATAGCTCACC ATGGCACCTT CATGGAGGAG 
TTCAATTCGG GGAAGCAGTT TGTTCGCTAT ATAAACATGC TGATAAATGA CACGACCTTT 
TTACTCGATG AAAGCCTGGA GTCTCTGAAG CGAATCCACG AAGTACAAGA AGAAATGAAG 
AACAAAGAAC AGTGGGACCA GCTGCCCCGG GATCAGCAGC AAGCCCGGCA GTCCCAGCTT 
GCTCAGGACG AGCGGGTTTC ACGCTCCTAC CTTGCCCTGG CAACAGAGAC CGTGGACATG 
TTCCATATCC TCACGAAACA GGTCCAGAAG CCCTTCCTCA GACCGGAACT TGGACCCCGA 
TTGGCTGCAA TGCTGAACTT TAATCTCCAG CAACTCTGTG GCCCCAAGTG CCGCGACCTG 
AAAGTTGAAA ACCCTGAGAA ATATGGCTTT GAACCAAAGA AGCTGTTGGA CCAACTGACA 
GACATTTACC TGCAGCTGGA CTGTGCCCGG TTTGCCAAAG CCATCGCTGA TGACCAGAGA 
TCCTACAGCA AAGAGTTGTT TGAAGAAGTG ATCTCGAAGA TGCGGAAGGC AGGGATCAAA 
TCCACAATAG CTATCGAGAA GTTTAAACTC CTCGCTGAGA AGGTGGAGGA GATAGTGGCC 
AAGAACGCAC GCGCAGAAAT CGACTACAGC GACGCCCCCG ACGAGTTCAG AGACCCACTG 
ATGGACACCC TGATGACTGA CCCTGTGCGG CTGCCCTCTG GCACCATCAT GGACCGCTCC 
ATCATCCTGC GGCACTTGCT GAACTCCCCC ACTGACCCCT TCAATCGACA GATGCTGACA 
GAGAACATGC TGGAACCAGT GCCAGAACTG AAAGAACAGA TTCACGCCTG GATGAGAGAG 
AAACAAAATA GTGATCATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE4B ( XM_844731.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_849824.2 Canis lupus familiaris ubiquitination factor E4B (UBE4B), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe06878
Clone ID Related Accession (Same CDS sequence)	XM_844731.4 , XM_844731.5
Accession Version	XM_844731.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	ubiquitin conjugation factor E4 B isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726059.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_844731.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGAGCGCTGA TGAGATCCGA CGGAGGCGCC TTGCACGACT TGCTGGTGGA 
CAGACCTCCC AGCCAACCAC CCCACTCACC TCGCCCCAGA GGGAGAACCC TCTGGGGCCT 
CCTATCGCAG CATCAGCCCC AGGCCCTTCC CAGAGCCTTG GTCTCAATGT CCACAACATG 
ACCCCAGCTA CCTCCCCTAT AGGTGCATCA GGAGTAGCTC ACCGAAGCCA GAGCAGTGAA 
GGAGTCAGTT CTCTCAGCAG CTCGCCCTCC AATAGCCTTG AAACCCAATC TCAGTCTCTC 
TCACGTTCCC AGAGCATGGA TATCGATGGG GTCTCTTGTG AAAAAAGCAT GTCCCAGGTG 
GATGTGGATT CAGGAATAGA AAATATGGAG GTTGATGAAA ACGATCGAAG AGAAAAGAGG 
AGCCTCAGCG ATAAGGAGCC TTCCTCGGGT TCTGAAGTAT CTGAAGAACA GGCCTTACAG 
CTGGTCTGTA AGATCTTTCG TGTCTCCTGG AAGGACCGGG ACAGAGATGT CATCTTTCTT 
TCTTCTCTTT CTGCACAATT TAAGCAGAAC CCAAAAGAAG TGTTCTCTGA TTTTAAGGAC 
TTGATTGGCC AGATTTTAAT GGAAGTGCTA ATGATGTCTA CGCAGACTAG AGATGAAAAT 
CCATTTGCCA GTCTGACAGC CACATCACAG CCAATTGCAG CAGCAGCTCG GTCACCAGAC 
AGGAATCTAA TGCTCACTAC TGGCTCTAAC CCAGGAACAA GCCCCATGTT CTGTGGTGTG 
GGTTCCTTTG GTGCCAGCTC TTTCTCTAGT TTGGGAGCCT CTGGTGGGGC AAGTACTTGG 
GATTCCTACA GCGACCATTT CACCATTGAG ACCTGCAAGG AGACAGACAT GCTGAACTAC 
CTCATCGAGT GTTTTGACCG AGTGGGAATA GAGGAAAAAA AAGCACCAAA GATGTGCAGC 
CAGCCAGCAG TCAGCCAGCT TCTCAGCAAC ATCCGCTCAC AGTGCATATC CCATACTGCG 
TTAGTCCTAC AAGGTTCCCT CACACAACCA AGGTCCATGC AGCAGCCATC CTTCCTGGTG 
CCGTATATGC TCTGCAGGAA TCTCCCATAT GGCTTCATGC AGGAACTGGT GAGAACCACT 
CACCAGGATG AAGAAGTGTT CAAGCAGATC TTTATCCCCA TTTTACAAGG CCTGGCTCTC 
GCTGCCAAAG AGTGCTCCCT CGATAGTGAC TACTTTAAGT ACCCCCTCAT GGCTTTAGGT 
GAGCTTTGTG AAACCAAGTT TGGGAAGACA CACCCCGTGT GCAATTTGGT GGCTTCTTTG 
CCCTTGTGGT TGCCGAAATC CTTAAGCCCT GGCTCTGGGC GGGAGCTGCA GAGACTCTCT 
TATTTGGGAG CTTTCTTTAG CTTCTCAGTC TTTGCGGAAG ATGACGCTAA AGTGGTTGAA 
AAATATTTCT CAGGGCCTGC CATTACCCTG GAAAACACTC GCGTGGTCAG CCAATCACTG 
CAGCATTACT TGGAGCTGGG AAGGCAAGAG CTTTTCAAGA TTCTGCATAG TATTCTGTTA 
AACGGTGAGA CCCGTGAGGC CGCTCTTGGT TACATGGCAG CTGTCGTCAA TGCCAATATG 
AAGAAAGCAC AGATGCAGAC AGATGATAGA TTGGTATCTA CAGATGGATT CATGCTGAAT 
TTCCTTTGGG TGCTGCAGCA GCTAAGTACA AAGATCAAGT TAGAGACGGT GGATCCCACG 
TATATATTCC ACCCAAGGTG TCGGATTACT CTTCCTAATG ACGAGACGAG AGTGAATGCT 
ACAATGGAGG ATGTGAATGA CTGGCTGGCT GAACTTTATG GAGACCAGCC TCCATTTTCT 
GAGCCGAAAT TTCCTACAGA ATGCTTTTTT CTTACCCTGC ATGCCCACCA CCTCTCCATT 
CTGCCAAGCT GCCGTCGCTA TATCCGTAGA CTGCGGGCCA TCCGGGAGCT CAATAGAACT 
GTGGAAGATT TGAAAAATAA TGAAAGTCAA TGGAAAGATT CCCCACTGGC AACCAGACAC 
CGTGAAATGC TGAAGCGCTG CAAAACTCAG CTTAAGAAAT TGGTGCGGTG CAAGGCCTGT 
GCTGATGCTG GTTTACTTGA TGAGAGCTTT CTGAGAAGAT GTCTGAATTT TTATGGCCTT 
CTCATCCAGC TGCTGCTCCG CATCCTGGAC CCCGCCTATC CCGATATAAC ACTGCCTTTA 
AACTCTGATG TCCCCAAGGT ATTTGCAGCA TTGCCTGAGT TTTATGTAGA AGATGTTGCT 
GAATTTTTAT TTTTTATTGT ACAATACTCT CCTCAGGTGC TGTATGAGCC CTGTACTCAG 
GATATTGTGA TGTTCCTCGT TGTGATGTTG TGTAACCAGA ACTACATCCG AAATCCATAT 
CTGGTGGCCA AACTGGTAGA AGTCATGTTT ATGACCAACC CAGCTGTTCA GCCACGAACC 
CAGAAGTTTT TTGAAATGAT TGAAAACCAT CCTCTCTCCA CCAAGTTGTT GGTCCCTTCA 
CTGATGAAGT TTTATACAGA TGTTGAACAC ACCGGAGCCA CCAGTGAGTT CTACGACAAG 
TTCACTATTC GCTATCACAT TAGCACCATT TTTAAAAGCC TTTGGCAAAA CATAGCTCAC 
CATGGCACCT TCATGGAGGA GTTCAATTCG GGGAAGCAGT TTGTTCGCTA TATAAACATG 
CTGATAAATG ACACGACCTT TTTACTCGAT GAAAGCCTGG AGTCTCTGAA GCGAATCCAC 
GAAGTACAAG AAGAAATGAA GAACAAAGAA CAGTGGGACC AGCTGCCCCG GGATCAGCAG 
CAAGCCCGGC AGTCCCAGCT TGCTCAGGAC GAGCGGGTTT CACGCTCCTA CCTTGCCCTG 
GCAACAGAGA CCGTGGACAT GTTCCATATC CTCACGAAAC AGGTCCAGAA GCCCTTCCTC 
AGACCGGAAC TTGGACCCCG ATTGGCTGCA ATGCTGAACT TTAATCTCCA GCAACTCTGT 
GGCCCCAAGT GCCGCGACCT GAAAGTTGAA AACCCTGAGA AATATGGCTT TGAACCAAAG 
AAGCTGTTGG ACCAACTGAC AGACATTTAC CTGCAGCTGG ACTGTGCCCG GTTTGCCAAA 
GCCATCGCTG ATGACCAGAG ATCCTACAGC AAAGAGTTGT TTGAAGAAGT GATCTCGAAG 
ATGCGGAAGG CAGGGATCAA ATCCACAATA GCTATCGAGA AGTTTAAACT CCTCGCTGAG 
AAGGTGGAGG AGATAGTGGC CAAGAACGCA CGCGCAGAAA TCGACTACAG CGACGCCCCC 
GACGAGTTCA GAGACCCACT GATGGACACC CTGATGACTG ACCCTGTGCG GCTGCCCTCT 
GGCACCATCA TGGACCGCTC CATCATCCTG CGGCACTTGC TGAACTCCCC CACTGACCCC 
TTCAATCGAC AGATGCTGAC AGAGAACATG CTGGAACCAG TGCCAGAACT GAAAGAACAG 
ATTCACGCCT GGATGAGAGA GAAACAAAAT AGTGATCATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_849824.2
CDS	726..4247
Translation	MEELSADEIRRRRLARLAGGQTSQPTTPLTSPQRENPLGPPIAASAPGPSQSLGLNVHNMTPATSPIGASGVAHRSQSSEGVSSLSSSPSNSLETQSQSLSRSQSMDIDGVSCEKSMSQVDVDSGIENMEVDENDRREKRSLSDKEPSSGSEVSEEQALQLVCKIFRVSWKDRDRDVIFLSSLSAQFKQNPKEVFSDFKDLIGQILMEVLMMSTQTRDENPFASLTATSQPIAAAARSPDRNLMLTTGSNPGTSPMFCGVGSFGASSFSSLGASGGASTWDSYSDHFTIETCKETDMLNYLIECFDRVGIEEKKAPKMCSQPAVSQLLSNIRSQCISHTALVLQGSLTQPRSMQQPSFLVPYMLCRNLPYGFMQELVRTTHQDEEVFKQIFIPILQGLALAAKECSLDSDYFKYPLMALGELCETKFGKTHPVCNLVASLPLWLPKSLSPGSGRELQRLSYLGAFFSFSVFAEDDAKVVEKYFSGPAITLENTRVVSQSLQHYLELGRQELFKILHSILLNGETREAALGYMAAVVNANMKKAQMQTDDRLVSTDGFMLNFLWVLQQLSTKIKLETVDPTYIFHPRCRITLPNDETRVNATMEDVNDWLAELYGDQPPFSEPKFPTECFFLTLHAHHLSILPSCRRYIRRLRAIRELNRTVEDLKNNESQWKDSPLATRHREMLKRCKTQLKKLVRCKACADAGLLDESFLRRCLNFYGLLIQLLLRILDPAYPDITLPLNSDVPKVFAALPEFYVEDVAEFLFFIVQYSPQVLYEPCTQDIVMFLVVMLCNQNYIRNPYLVAKLVEVMFMTNPAVQPRTQKFFEMIENHPLSTKLLVPSLMKFYTDVEHTGATSEFYDKFTIRYHISTIFKSLWQNIAHHGTFMEEFNSGKQFVRYINMLINDTTFLLDESLESLKRIHEVQEEMKNKEQWDQLPRDQQQARQSQLAQDERVSRSYLALATETVDMFHILTKQVQKPFLRPELGPRLAAMLNFNLQQLCGPKCRDLKVENPEKYGFEPKKLLDQLTDIYLQLDCARFAKAIADDQRSYSKELFEEVISKMRKAGIKSTIAIEKFKLLAEKVEEIVAKNARAEIDYSDAPDEFRDPLMDTLMTDPVRLPSGTIMDRSIILRHLLNSPTDPFNRQMLTENMLEPVPELKEQIHAWMREKQNSDH

Target ORF information:

RefSeq Version	XM_844731.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ubiquitination factor E4B (UBE4B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_844731.4

ORF Insert Sequence:

ATGGAGGAGC TGAGCGCTGA TGAGATCCGA CGGAGGCGCC TTGCACGACT TGCTGGTGGA 
CAGACCTCCC AGCCAACCAC CCCACTCACC TCGCCCCAGA GGGAGAACCC TCTGGGGCCT 
CCTATCGCAG CATCAGCCCC AGGCCCTTCC CAGAGCCTTG GTCTCAATGT CCACAACATG 
ACCCCAGCTA CCTCCCCTAT AGGTGCATCA GGAGTAGCTC ACCGAAGCCA GAGCAGTGAA 
GGAGTCAGTT CTCTCAGCAG CTCGCCCTCC AATAGCCTTG AAACCCAATC TCAGTCTCTC 
TCACGTTCCC AGAGCATGGA TATCGATGGG GTCTCTTGTG AAAAAAGCAT GTCCCAGGTG 
GATGTGGATT CAGGAATAGA AAATATGGAG GTTGATGAAA ACGATCGAAG AGAAAAGAGG 
AGCCTCAGCG ATAAGGAGCC TTCCTCGGGT TCTGAAGTAT CTGAAGAACA GGCCTTACAG 
CTGGTCTGTA AGATCTTTCG TGTCTCCTGG AAGGACCGGG ACAGAGATGT CATCTTTCTT 
TCTTCTCTTT CTGCACAATT TAAGCAGAAC CCAAAAGAAG TGTTCTCTGA TTTTAAGGAC 
TTGATTGGCC AGATTTTAAT GGAAGTGCTA ATGATGTCTA CGCAGACTAG AGATGAAAAT 
CCATTTGCCA GTCTGACAGC CACATCACAG CCAATTGCAG CAGCAGCTCG GTCACCAGAC 
AGGAATCTAA TGCTCACTAC TGGCTCTAAC CCAGGAACAA GCCCCATGTT CTGTGGTGTG 
GGTTCCTTTG GTGCCAGCTC TTTCTCTAGT TTGGGAGCCT CTGGTGGGGC AAGTACTTGG 
GATTCCTACA GCGACCATTT CACCATTGAG ACCTGCAAGG AGACAGACAT GCTGAACTAC 
CTCATCGAGT GTTTTGACCG AGTGGGAATA GAGGAAAAAA AAGCACCAAA GATGTGCAGC 
CAGCCAGCAG TCAGCCAGCT TCTCAGCAAC ATCCGCTCAC AGTGCATATC CCATACTGCG 
TTAGTCCTAC AAGGTTCCCT CACACAACCA AGGTCCATGC AGCAGCCATC CTTCCTGGTG 
CCGTATATGC TCTGCAGGAA TCTCCCATAT GGCTTCATGC AGGAACTGGT GAGAACCACT 
CACCAGGATG AAGAAGTGTT CAAGCAGATC TTTATCCCCA TTTTACAAGG CCTGGCTCTC 
GCTGCCAAAG AGTGCTCCCT CGATAGTGAC TACTTTAAGT ACCCCCTCAT GGCTTTAGGT 
GAGCTTTGTG AAACCAAGTT TGGGAAGACA CACCCCGTGT GCAATTTGGT GGCTTCTTTG 
CCCTTGTGGT TGCCGAAATC CTTAAGCCCT GGCTCTGGGC GGGAGCTGCA GAGACTCTCT 
TATTTGGGAG CTTTCTTTAG CTTCTCAGTC TTTGCGGAAG ATGACGCTAA AGTGGTTGAA 
AAATATTTCT CAGGGCCTGC CATTACCCTG GAAAACACTC GCGTGGTCAG CCAATCACTG 
CAGCATTACT TGGAGCTGGG AAGGCAAGAG CTTTTCAAGA TTCTGCATAG TATTCTGTTA 
AACGGTGAGA CCCGTGAGGC CGCTCTTGGT TACATGGCAG CTGTCGTCAA TGCCAATATG 
AAGAAAGCAC AGATGCAGAC AGATGATAGA TTGGTATCTA CAGATGGATT CATGCTGAAT 
TTCCTTTGGG TGCTGCAGCA GCTAAGTACA AAGATCAAGT TAGAGACGGT GGATCCCACG 
TATATATTCC ACCCAAGGTG TCGGATTACT CTTCCTAATG ACGAGACGAG AGTGAATGCT 
ACAATGGAGG ATGTGAATGA CTGGCTGGCT GAACTTTATG GAGACCAGCC TCCATTTTCT 
GAGCCGAAAT TTCCTACAGA ATGCTTTTTT CTTACCCTGC ATGCCCACCA CCTCTCCATT 
CTGCCAAGCT GCCGTCGCTA TATCCGTAGA CTGCGGGCCA TCCGGGAGCT CAATAGAACT 
GTGGAAGATT TGAAAAATAA TGAAAGTCAA TGGAAAGATT CCCCACTGGC AACCAGACAC 
CGTGAAATGC TGAAGCGCTG CAAAACTCAG CTTAAGAAAT TGGTGCGGTG CAAGGCCTGT 
GCTGATGCTG GTTTACTTGA TGAGAGCTTT CTGAGAAGAT GTCTGAATTT TTATGGCCTT 
CTCATCCAGC TGCTGCTCCG CATCCTGGAC CCCGCCTATC CCGATATAAC ACTGCCTTTA 
AACTCTGATG TCCCCAAGGT ATTTGCAGCA TTGCCTGAGT TTTATGTAGA AGATGTTGCT 
GAATTTTTAT TTTTTATTGT ACAATACTCT CCTCAGGTGC TGTATGAGCC CTGTACTCAG 
GATATTGTGA TGTTCCTCGT TGTGATGTTG TGTAACCAGA ACTACATCCG AAATCCATAT 
CTGGTGGCCA AACTGGTAGA AGTCATGTTT ATGACCAACC CAGCTGTTCA GCCACGAACC 
CAGAAGTTTT TTGAAATGAT TGAAAACCAT CCTCTCTCCA CCAAGTTGTT GGTCCCTTCA 
CTGATGAAGT TTTATACAGA TGTTGAACAC ACCGGAGCCA CCAGTGAGTT CTACGACAAG 
TTCACTATTC GCTATCACAT TAGCACCATT TTTAAAAGCC TTTGGCAAAA CATAGCTCAC 
CATGGCACCT TCATGGAGGA GTTCAATTCG GGGAAGCAGT TTGTTCGCTA TATAAACATG 
CTGATAAATG ACACGACCTT TTTACTCGAT GAAAGCCTGG AGTCTCTGAA GCGAATCCAC 
GAAGTACAAG AAGAAATGAA GAACAAAGAA CAGTGGGACC AGCTGCCCCG GGATCAGCAG 
CAAGCCCGGC AGTCCCAGCT TGCTCAGGAC GAGCGGGTTT CACGCTCCTA CCTTGCCCTG 
GCAACAGAGA CCGTGGACAT GTTCCATATC CTCACGAAAC AGGTCCAGAA GCCCTTCCTC 
AGACCGGAAC TTGGACCCCG ATTGGCTGCA ATGCTGAACT TTAATCTCCA GCAACTCTGT 
GGCCCCAAGT GCCGCGACCT GAAAGTTGAA AACCCTGAGA AATATGGCTT TGAACCAAAG 
AAGCTGTTGG ACCAACTGAC AGACATTTAC CTGCAGCTGG ACTGTGCCCG GTTTGCCAAA 
GCCATCGCTG ATGACCAGAG ATCCTACAGC AAAGAGTTGT TTGAAGAAGT GATCTCGAAG 
ATGCGGAAGG CAGGGATCAA ATCCACAATA GCTATCGAGA AGTTTAAACT CCTCGCTGAG 
AAGGTGGAGG AGATAGTGGC CAAGAACGCA CGCGCAGAAA TCGACTACAG CGACGCCCCC 
GACGAGTTCA GAGACCCACT GATGGACACC CTGATGACTG ACCCTGTGCG GCTGCCCTCT 
GGCACCATCA TGGACCGCTC CATCATCCTG CGGCACTTGC TGAACTCCCC CACTGACCCC 
TTCAATCGAC AGATGCTGAC AGAGAACATG CTGGAACCAG TGCCAGAACT GAAAGAACAG 
ATTCACGCCT GGATGAGAGA GAAACAAAAT AGTGATCATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE4B ( XM_844731.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_849824.2 Canis lupus familiaris ubiquitination factor E4B (UBE4B), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe06878
Clone ID Related Accession (Same CDS sequence)	XM_844731.4 , XM_844731.5
Accession Version	XM_844731.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	ubiquitin conjugation factor E4 B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_844731.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGAGCGCTGA TGAGATCCGA CGGAGGCGCC TTGCACGACT TGCTGGTGGA 
CAGACCTCCC AGCCAACCAC CCCACTCACC TCGCCCCAGA GGGAGAACCC TCTGGGGCCT 
CCTATCGCAG CATCAGCCCC AGGCCCTTCC CAGAGCCTTG GTCTCAATGT CCACAACATG 
ACCCCAGCTA CCTCCCCTAT AGGTGCATCA GGAGTAGCTC ACCGAAGCCA GAGCAGTGAA 
GGAGTCAGTT CTCTCAGCAG CTCGCCCTCC AATAGCCTTG AAACCCAATC TCAGTCTCTC 
TCACGTTCCC AGAGCATGGA TATCGATGGG GTCTCTTGTG AAAAAAGCAT GTCCCAGGTG 
GATGTGGATT CAGGAATAGA AAATATGGAG GTTGATGAAA ACGATCGAAG AGAAAAGAGG 
AGCCTCAGCG ATAAGGAGCC TTCCTCGGGT TCTGAAGTAT CTGAAGAACA GGCCTTACAG 
CTGGTCTGTA AGATCTTTCG TGTCTCCTGG AAGGACCGGG ACAGAGATGT CATCTTTCTT 
TCTTCTCTTT CTGCACAATT TAAGCAGAAC CCAAAAGAAG TGTTCTCTGA TTTTAAGGAC 
TTGATTGGCC AGATTTTAAT GGAAGTGCTA ATGATGTCTA CGCAGACTAG AGATGAAAAT 
CCATTTGCCA GTCTGACAGC CACATCACAG CCAATTGCAG CAGCAGCTCG GTCACCAGAC 
AGGAATCTAA TGCTCACTAC TGGCTCTAAC CCAGGAACAA GCCCCATGTT CTGTGGTGTG 
GGTTCCTTTG GTGCCAGCTC TTTCTCTAGT TTGGGAGCCT CTGGTGGGGC AAGTACTTGG 
GATTCCTACA GCGACCATTT CACCATTGAG ACCTGCAAGG AGACAGACAT GCTGAACTAC 
CTCATCGAGT GTTTTGACCG AGTGGGAATA GAGGAAAAAA AAGCACCAAA GATGTGCAGC 
CAGCCAGCAG TCAGCCAGCT TCTCAGCAAC ATCCGCTCAC AGTGCATATC CCATACTGCG 
TTAGTCCTAC AAGGTTCCCT CACACAACCA AGGTCCATGC AGCAGCCATC CTTCCTGGTG 
CCGTATATGC TCTGCAGGAA TCTCCCATAT GGCTTCATGC AGGAACTGGT GAGAACCACT 
CACCAGGATG AAGAAGTGTT CAAGCAGATC TTTATCCCCA TTTTACAAGG CCTGGCTCTC 
GCTGCCAAAG AGTGCTCCCT CGATAGTGAC TACTTTAAGT ACCCCCTCAT GGCTTTAGGT 
GAGCTTTGTG AAACCAAGTT TGGGAAGACA CACCCCGTGT GCAATTTGGT GGCTTCTTTG 
CCCTTGTGGT TGCCGAAATC CTTAAGCCCT GGCTCTGGGC GGGAGCTGCA GAGACTCTCT 
TATTTGGGAG CTTTCTTTAG CTTCTCAGTC TTTGCGGAAG ATGACGCTAA AGTGGTTGAA 
AAATATTTCT CAGGGCCTGC CATTACCCTG GAAAACACTC GCGTGGTCAG CCAATCACTG 
CAGCATTACT TGGAGCTGGG AAGGCAAGAG CTTTTCAAGA TTCTGCATAG TATTCTGTTA 
AACGGTGAGA CCCGTGAGGC CGCTCTTGGT TACATGGCAG CTGTCGTCAA TGCCAATATG 
AAGAAAGCAC AGATGCAGAC AGATGATAGA TTGGTATCTA CAGATGGATT CATGCTGAAT 
TTCCTTTGGG TGCTGCAGCA GCTAAGTACA AAGATCAAGT TAGAGACGGT GGATCCCACG 
TATATATTCC ACCCAAGGTG TCGGATTACT CTTCCTAATG ACGAGACGAG AGTGAATGCT 
ACAATGGAGG ATGTGAATGA CTGGCTGGCT GAACTTTATG GAGACCAGCC TCCATTTTCT 
GAGCCGAAAT TTCCTACAGA ATGCTTTTTT CTTACCCTGC ATGCCCACCA CCTCTCCATT 
CTGCCAAGCT GCCGTCGCTA TATCCGTAGA CTGCGGGCCA TCCGGGAGCT CAATAGAACT 
GTGGAAGATT TGAAAAATAA TGAAAGTCAA TGGAAAGATT CCCCACTGGC AACCAGACAC 
CGTGAAATGC TGAAGCGCTG CAAAACTCAG CTTAAGAAAT TGGTGCGGTG CAAGGCCTGT 
GCTGATGCTG GTTTACTTGA TGAGAGCTTT CTGAGAAGAT GTCTGAATTT TTATGGCCTT 
CTCATCCAGC TGCTGCTCCG CATCCTGGAC CCCGCCTATC CCGATATAAC ACTGCCTTTA 
AACTCTGATG TCCCCAAGGT ATTTGCAGCA TTGCCTGAGT TTTATGTAGA AGATGTTGCT 
GAATTTTTAT TTTTTATTGT ACAATACTCT CCTCAGGTGC TGTATGAGCC CTGTACTCAG 
GATATTGTGA TGTTCCTCGT TGTGATGTTG TGTAACCAGA ACTACATCCG AAATCCATAT 
CTGGTGGCCA AACTGGTAGA AGTCATGTTT ATGACCAACC CAGCTGTTCA GCCACGAACC 
CAGAAGTTTT TTGAAATGAT TGAAAACCAT CCTCTCTCCA CCAAGTTGTT GGTCCCTTCA 
CTGATGAAGT TTTATACAGA TGTTGAACAC ACCGGAGCCA CCAGTGAGTT CTACGACAAG 
TTCACTATTC GCTATCACAT TAGCACCATT TTTAAAAGCC TTTGGCAAAA CATAGCTCAC 
CATGGCACCT TCATGGAGGA GTTCAATTCG GGGAAGCAGT TTGTTCGCTA TATAAACATG 
CTGATAAATG ACACGACCTT TTTACTCGAT GAAAGCCTGG AGTCTCTGAA GCGAATCCAC 
GAAGTACAAG AAGAAATGAA GAACAAAGAA CAGTGGGACC AGCTGCCCCG GGATCAGCAG 
CAAGCCCGGC AGTCCCAGCT TGCTCAGGAC GAGCGGGTTT CACGCTCCTA CCTTGCCCTG 
GCAACAGAGA CCGTGGACAT GTTCCATATC CTCACGAAAC AGGTCCAGAA GCCCTTCCTC 
AGACCGGAAC TTGGACCCCG ATTGGCTGCA ATGCTGAACT TTAATCTCCA GCAACTCTGT 
GGCCCCAAGT GCCGCGACCT GAAAGTTGAA AACCCTGAGA AATATGGCTT TGAACCAAAG 
AAGCTGTTGG ACCAACTGAC AGACATTTAC CTGCAGCTGG ACTGTGCCCG GTTTGCCAAA 
GCCATCGCTG ATGACCAGAG ATCCTACAGC AAAGAGTTGT TTGAAGAAGT GATCTCGAAG 
ATGCGGAAGG CAGGGATCAA ATCCACAATA GCTATCGAGA AGTTTAAACT CCTCGCTGAG 
AAGGTGGAGG AGATAGTGGC CAAGAACGCA CGCGCAGAAA TCGACTACAG CGACGCCCCC 
GACGAGTTCA GAGACCCACT GATGGACACC CTGATGACTG ACCCTGTGCG GCTGCCCTCT 
GGCACCATCA TGGACCGCTC CATCATCCTG CGGCACTTGC TGAACTCCCC CACTGACCCC 
TTCAATCGAC AGATGCTGAC AGAGAACATG CTGGAACCAG TGCCAGAACT GAAAGAACAG 
ATTCACGCCT GGATGAGAGA GAAACAAAAT AGTGATCATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_849824.2
CDS	715..4236
Translation	MEELSADEIRRRRLARLAGGQTSQPTTPLTSPQRENPLGPPIAASAPGPSQSLGLNVHNMTPATSPIGASGVAHRSQSSEGVSSLSSSPSNSLETQSQSLSRSQSMDIDGVSCEKSMSQVDVDSGIENMEVDENDRREKRSLSDKEPSSGSEVSEEQALQLVCKIFRVSWKDRDRDVIFLSSLSAQFKQNPKEVFSDFKDLIGQILMEVLMMSTQTRDENPFASLTATSQPIAAAARSPDRNLMLTTGSNPGTSPMFCGVGSFGASSFSSLGASGGASTWDSYSDHFTIETCKETDMLNYLIECFDRVGIEEKKAPKMCSQPAVSQLLSNIRSQCISHTALVLQGSLTQPRSMQQPSFLVPYMLCRNLPYGFMQELVRTTHQDEEVFKQIFIPILQGLALAAKECSLDSDYFKYPLMALGELCETKFGKTHPVCNLVASLPLWLPKSLSPGSGRELQRLSYLGAFFSFSVFAEDDAKVVEKYFSGPAITLENTRVVSQSLQHYLELGRQELFKILHSILLNGETREAALGYMAAVVNANMKKAQMQTDDRLVSTDGFMLNFLWVLQQLSTKIKLETVDPTYIFHPRCRITLPNDETRVNATMEDVNDWLAELYGDQPPFSEPKFPTECFFLTLHAHHLSILPSCRRYIRRLRAIRELNRTVEDLKNNESQWKDSPLATRHREMLKRCKTQLKKLVRCKACADAGLLDESFLRRCLNFYGLLIQLLLRILDPAYPDITLPLNSDVPKVFAALPEFYVEDVAEFLFFIVQYSPQVLYEPCTQDIVMFLVVMLCNQNYIRNPYLVAKLVEVMFMTNPAVQPRTQKFFEMIENHPLSTKLLVPSLMKFYTDVEHTGATSEFYDKFTIRYHISTIFKSLWQNIAHHGTFMEEFNSGKQFVRYINMLINDTTFLLDESLESLKRIHEVQEEMKNKEQWDQLPRDQQQARQSQLAQDERVSRSYLALATETVDMFHILTKQVQKPFLRPELGPRLAAMLNFNLQQLCGPKCRDLKVENPEKYGFEPKKLLDQLTDIYLQLDCARFAKAIADDQRSYSKELFEEVISKMRKAGIKSTIAIEKFKLLAEKVEEIVAKNARAEIDYSDAPDEFRDPLMDTLMTDPVRLPSGTIMDRSIILRHLLNSPTDPFNRQMLTENMLEPVPELKEQIHAWMREKQNSDH

Target ORF information:

RefSeq Version	XM_844731.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ubiquitination factor E4B (UBE4B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_844731.5

ORF Insert Sequence:

ATGGAGGAGC TGAGCGCTGA TGAGATCCGA CGGAGGCGCC TTGCACGACT TGCTGGTGGA 
CAGACCTCCC AGCCAACCAC CCCACTCACC TCGCCCCAGA GGGAGAACCC TCTGGGGCCT 
CCTATCGCAG CATCAGCCCC AGGCCCTTCC CAGAGCCTTG GTCTCAATGT CCACAACATG 
ACCCCAGCTA CCTCCCCTAT AGGTGCATCA GGAGTAGCTC ACCGAAGCCA GAGCAGTGAA 
GGAGTCAGTT CTCTCAGCAG CTCGCCCTCC AATAGCCTTG AAACCCAATC TCAGTCTCTC 
TCACGTTCCC AGAGCATGGA TATCGATGGG GTCTCTTGTG AAAAAAGCAT GTCCCAGGTG 
GATGTGGATT CAGGAATAGA AAATATGGAG GTTGATGAAA ACGATCGAAG AGAAAAGAGG 
AGCCTCAGCG ATAAGGAGCC TTCCTCGGGT TCTGAAGTAT CTGAAGAACA GGCCTTACAG 
CTGGTCTGTA AGATCTTTCG TGTCTCCTGG AAGGACCGGG ACAGAGATGT CATCTTTCTT 
TCTTCTCTTT CTGCACAATT TAAGCAGAAC CCAAAAGAAG TGTTCTCTGA TTTTAAGGAC 
TTGATTGGCC AGATTTTAAT GGAAGTGCTA ATGATGTCTA CGCAGACTAG AGATGAAAAT 
CCATTTGCCA GTCTGACAGC CACATCACAG CCAATTGCAG CAGCAGCTCG GTCACCAGAC 
AGGAATCTAA TGCTCACTAC TGGCTCTAAC CCAGGAACAA GCCCCATGTT CTGTGGTGTG 
GGTTCCTTTG GTGCCAGCTC TTTCTCTAGT TTGGGAGCCT CTGGTGGGGC AAGTACTTGG 
GATTCCTACA GCGACCATTT CACCATTGAG ACCTGCAAGG AGACAGACAT GCTGAACTAC 
CTCATCGAGT GTTTTGACCG AGTGGGAATA GAGGAAAAAA AAGCACCAAA GATGTGCAGC 
CAGCCAGCAG TCAGCCAGCT TCTCAGCAAC ATCCGCTCAC AGTGCATATC CCATACTGCG 
TTAGTCCTAC AAGGTTCCCT CACACAACCA AGGTCCATGC AGCAGCCATC CTTCCTGGTG 
CCGTATATGC TCTGCAGGAA TCTCCCATAT GGCTTCATGC AGGAACTGGT GAGAACCACT 
CACCAGGATG AAGAAGTGTT CAAGCAGATC TTTATCCCCA TTTTACAAGG CCTGGCTCTC 
GCTGCCAAAG AGTGCTCCCT CGATAGTGAC TACTTTAAGT ACCCCCTCAT GGCTTTAGGT 
GAGCTTTGTG AAACCAAGTT TGGGAAGACA CACCCCGTGT GCAATTTGGT GGCTTCTTTG 
CCCTTGTGGT TGCCGAAATC CTTAAGCCCT GGCTCTGGGC GGGAGCTGCA GAGACTCTCT 
TATTTGGGAG CTTTCTTTAG CTTCTCAGTC TTTGCGGAAG ATGACGCTAA AGTGGTTGAA 
AAATATTTCT CAGGGCCTGC CATTACCCTG GAAAACACTC GCGTGGTCAG CCAATCACTG 
CAGCATTACT TGGAGCTGGG AAGGCAAGAG CTTTTCAAGA TTCTGCATAG TATTCTGTTA 
AACGGTGAGA CCCGTGAGGC CGCTCTTGGT TACATGGCAG CTGTCGTCAA TGCCAATATG 
AAGAAAGCAC AGATGCAGAC AGATGATAGA TTGGTATCTA CAGATGGATT CATGCTGAAT 
TTCCTTTGGG TGCTGCAGCA GCTAAGTACA AAGATCAAGT TAGAGACGGT GGATCCCACG 
TATATATTCC ACCCAAGGTG TCGGATTACT CTTCCTAATG ACGAGACGAG AGTGAATGCT 
ACAATGGAGG ATGTGAATGA CTGGCTGGCT GAACTTTATG GAGACCAGCC TCCATTTTCT 
GAGCCGAAAT TTCCTACAGA ATGCTTTTTT CTTACCCTGC ATGCCCACCA CCTCTCCATT 
CTGCCAAGCT GCCGTCGCTA TATCCGTAGA CTGCGGGCCA TCCGGGAGCT CAATAGAACT 
GTGGAAGATT TGAAAAATAA TGAAAGTCAA TGGAAAGATT CCCCACTGGC AACCAGACAC 
CGTGAAATGC TGAAGCGCTG CAAAACTCAG CTTAAGAAAT TGGTGCGGTG CAAGGCCTGT 
GCTGATGCTG GTTTACTTGA TGAGAGCTTT CTGAGAAGAT GTCTGAATTT TTATGGCCTT 
CTCATCCAGC TGCTGCTCCG CATCCTGGAC CCCGCCTATC CCGATATAAC ACTGCCTTTA 
AACTCTGATG TCCCCAAGGT ATTTGCAGCA TTGCCTGAGT TTTATGTAGA AGATGTTGCT 
GAATTTTTAT TTTTTATTGT ACAATACTCT CCTCAGGTGC TGTATGAGCC CTGTACTCAG 
GATATTGTGA TGTTCCTCGT TGTGATGTTG TGTAACCAGA ACTACATCCG AAATCCATAT 
CTGGTGGCCA AACTGGTAGA AGTCATGTTT ATGACCAACC CAGCTGTTCA GCCACGAACC 
CAGAAGTTTT TTGAAATGAT TGAAAACCAT CCTCTCTCCA CCAAGTTGTT GGTCCCTTCA 
CTGATGAAGT TTTATACAGA TGTTGAACAC ACCGGAGCCA CCAGTGAGTT CTACGACAAG 
TTCACTATTC GCTATCACAT TAGCACCATT TTTAAAAGCC TTTGGCAAAA CATAGCTCAC 
CATGGCACCT TCATGGAGGA GTTCAATTCG GGGAAGCAGT TTGTTCGCTA TATAAACATG 
CTGATAAATG ACACGACCTT TTTACTCGAT GAAAGCCTGG AGTCTCTGAA GCGAATCCAC 
GAAGTACAAG AAGAAATGAA GAACAAAGAA CAGTGGGACC AGCTGCCCCG GGATCAGCAG 
CAAGCCCGGC AGTCCCAGCT TGCTCAGGAC GAGCGGGTTT CACGCTCCTA CCTTGCCCTG 
GCAACAGAGA CCGTGGACAT GTTCCATATC CTCACGAAAC AGGTCCAGAA GCCCTTCCTC 
AGACCGGAAC TTGGACCCCG ATTGGCTGCA ATGCTGAACT TTAATCTCCA GCAACTCTGT 
GGCCCCAAGT GCCGCGACCT GAAAGTTGAA AACCCTGAGA AATATGGCTT TGAACCAAAG 
AAGCTGTTGG ACCAACTGAC AGACATTTAC CTGCAGCTGG ACTGTGCCCG GTTTGCCAAA 
GCCATCGCTG ATGACCAGAG ATCCTACAGC AAAGAGTTGT TTGAAGAAGT GATCTCGAAG 
ATGCGGAAGG CAGGGATCAA ATCCACAATA GCTATCGAGA AGTTTAAACT CCTCGCTGAG 
AAGGTGGAGG AGATAGTGGC CAAGAACGCA CGCGCAGAAA TCGACTACAG CGACGCCCCC 
GACGAGTTCA GAGACCCACT GATGGACACC CTGATGACTG ACCCTGTGCG GCTGCCCTCT 
GGCACCATCA TGGACCGCTC CATCATCCTG CGGCACTTGC TGAACTCCCC CACTGACCCC 
TTCAATCGAC AGATGCTGAC AGAGAACATG CTGGAACCAG TGCCAGAACT GAAAGAACAG 
ATTCACGCCT GGATGAGAGA GAAACAAAAT AGTGATCATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE4B ( XM_005620526.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005620583.1 Canis lupus familiaris ubiquitination factor E4B (UBE4B), transcript variant X1, mRNA.

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CloneID	OCe06876
Clone ID Related Accession (Same CDS sequence)	XM_005620526.3 , XM_005620526.2
Accession Version	XM_005620526.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	ubiquitin conjugation factor E4 B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005620526.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGAGCGCTGA TGAGATCCGA CGGAGGCGCC TTGCACGACT TGCTGGTGGA 
CAGACCTCCC AGCCAACCAC CCCACTCACC TCGCCCCAGA GGGAGAACCC TCTGGGGCCT 
CCTATCGCAG CATCAGCCCC AGGCCCTTCC CAGAGCCTTG GTCTCAATGT CCACAACATG 
ACCCCAGCTA CCTCCCCTAT AGGTGCATCA GGAGTAGCTC ACCGAAGCCA GAGCAGTGAA 
GGAGTCAGTT CTCTCAGCAG CTCGCCCTCC AATAGCCTTG AAACCCAATC TCAGTCTCTC 
TCACGTTCCC AGAGCATGGA TATCGATGGG GTCTCTTGTG AAAAAAGCAT GTCCCAGGTG 
GATGTGGATT CAGGAATAGA AAATATGGAG GTTGATGAAA ACGATCGAAG AGAAAAGAGG 
AGCCTCAGCG ATAAGGAGCC TTCCTCGGGT TCTGAAGTAT CTGAAGAACA GGCCTTACAG 
CTGGTCTGTA AGATCTTTCG TGTCTCCTGG AAGGACCGGG ACAGAGATGT CATCTTTCTT 
TCTTCTCTTT CTGCACAATT TAAGCAGAAC CCAAAAGAAG TGTTCTCTGA TTTTAAGGAC 
TTGATTGGCC AGATTTTAAT GGAAGTGCTA ATGATGTCTA CGCAGACTAG AGATGAAAAT 
CCATTTGCCA GTCTGACAGC CACATCACAG CCAATTGCAG CAGCAGCTCG GTCACCAGAC 
AGGAATCTAA TGCTCACTAC TGGCTCTAAC CCAGGAACAA GCCCCATGTT CTGTGGTGTG 
GGTTCCTTTG GTGCCAGCTC TTTCTCTAGC CTCTATGAAA CTAGCCCAGC TCCCAGTACA 
AGTTTCTGGA GCTCGGTGCC AGTGATGAGT CCATCCTTTG CCTCCCCCCC TGCCCGTGTG 
GCTGGCCAGA TGGTTGTGCC CTCCATTTCC CTCAGCCCGC ATGGTGGCAT GGCCTCTGGA 
ACTGCCACAG GAAGCCAGCC CTCCTCTCCA CGGTATCGCC CCTACACTGT CACCCACCCG 
TGGGGGTCTT CGACTTCTCC TACCCCGCGA TCCCCAGGCA TCTCCATTCT GTCCAGCTCG 
CCCAGCCTCC CTGCCCTTGC TAGTAGCCCT CAAGCGGTTC CTGCCAGCTC TCTCAGACAG 
AGACCCAGCA GCCTGGGGCC CCCTTCAGTG GCCGCCTCAC CCAGTGCCGT GAGCAGACGT 
CCCTCATCCC TAAGGATCTC TCCTAGTATG TACGACAATC CTTTCTCCTT CCTCTTCCTC 
GCACTTTCTG GCGACAGTAG TGATGAAGAT GAAGAAGATG ATGATGATGA TGATGATGGT 
GATGATGAAG GTGGTGGTGG TGGTGATGAG TTTTCTTGTG TCCAGTTTGG GTCCAGTTTG 
GGAGCCTCTG GTGGGGCAAG TACTTGGGAT TCCTACAGCG ACCATTTCAC CATTGAGACC 
TGCAAGGAGA CAGACATGCT GAACTACCTC ATCGAGTGTT TTGACCGAGT GGGAATAGAG 
GAAAAAAAAG CACCAAAGAT GTGCAGCCAG CCAGCAGTCA GCCAGCTTCT CAGCAACATC 
CGCTCACAGT GCATATCCCA TACTGCGTTA GTCCTACAAG GTTCCCTCAC ACAACCAAGG 
TCCATGCAGC AGCCATCCTT CCTGGTGCCG TATATGCTCT GCAGGAATCT CCCATATGGC 
TTCATGCAGG AACTGGTGAG AACCACTCAC CAGGATGAAG AAGTGTTCAA GCAGATCTTT 
ATCCCCATTT TACAAGGCCT GGCTCTCGCT GCCAAAGAGT GCTCCCTCGA TAGTGACTAC 
TTTAAGTACC CCCTCATGGC TTTAGGTGAG CTTTGTGAAA CCAAGTTTGG GAAGACACAC 
CCCGTGTGCA ATTTGGTGGC TTCTTTGCCC TTGTGGTTGC CGAAATCCTT AAGCCCTGGC 
TCTGGGCGGG AGCTGCAGAG ACTCTCTTAT TTGGGAGCTT TCTTTAGCTT CTCAGTCTTT 
GCGGAAGATG ACGCTAAAGT GGTTGAAAAA TATTTCTCAG GGCCTGCCAT TACCCTGGAA 
AACACTCGCG TGGTCAGCCA ATCACTGCAG CATTACTTGG AGCTGGGAAG GCAAGAGCTT 
TTCAAGATTC TGCATAGTAT TCTGTTAAAC GGTGAGACCC GTGAGGCCGC TCTTGGTTAC 
ATGGCAGCTG TCGTCAATGC CAATATGAAG AAAGCACAGA TGCAGACAGA TGATAGATTG 
GTATCTACAG ATGGATTCAT GCTGAATTTC CTTTGGGTGC TGCAGCAGCT AAGTACAAAG 
ATCAAGTTAG AGACGGTGGA TCCCACGTAT ATATTCCACC CAAGGTGTCG GATTACTCTT 
CCTAATGACG AGACGAGAGT GAATGCTACA ATGGAGGATG TGAATGACTG GCTGGCTGAA 
CTTTATGGAG ACCAGCCTCC ATTTTCTGAG CCGAAATTTC CTACAGAATG CTTTTTTCTT 
ACCCTGCATG CCCACCACCT CTCCATTCTG CCAAGCTGCC GTCGCTATAT CCGTAGACTG 
CGGGCCATCC GGGAGCTCAA TAGAACTGTG GAAGATTTGA AAAATAATGA AAGTCAATGG 
AAAGATTCCC CACTGGCAAC CAGACACCGT GAAATGCTGA AGCGCTGCAA AACTCAGCTT 
AAGAAATTGG TGCGGTGCAA GGCCTGTGCT GATGCTGGTT TACTTGATGA GAGCTTTCTG 
AGAAGATGTC TGAATTTTTA TGGCCTTCTC ATCCAGCTGC TGCTCCGCAT CCTGGACCCC 
GCCTATCCCG ATATAACACT GCCTTTAAAC TCTGATGTCC CCAAGGTATT TGCAGCATTG 
CCTGAGTTTT ATGTAGAAGA TGTTGCTGAA TTTTTATTTT TTATTGTACA ATACTCTCCT 
CAGGTGCTGT ATGAGCCCTG TACTCAGGAT ATTGTGATGT TCCTCGTTGT GATGTTGTGT 
AACCAGAACT ACATCCGAAA TCCATATCTG GTGGCCAAAC TGGTAGAAGT CATGTTTATG 
ACCAACCCAG CTGTTCAGCC ACGAACCCAG AAGTTTTTTG AAATGATTGA AAACCATCCT 
CTCTCCACCA AGTTGTTGGT CCCTTCACTG ATGAAGTTTT ATACAGATGT TGAACACACC 
GGAGCCACCA GTGAGTTCTA CGACAAGTTC ACTATTCGCT ATCACATTAG CACCATTTTT 
AAAAGCCTTT GGCAAAACAT AGCTCACCAT GGCACCTTCA TGGAGGAGTT CAATTCGGGG 
AAGCAGTTTG TTCGCTATAT AAACATGCTG ATAAATGACA CGACCTTTTT ACTCGATGAA 
AGCCTGGAGT CTCTGAAGCG AATCCACGAA GTACAAGAAG AAATGAAGAA CAAAGAACAG 
TGGGACCAGC TGCCCCGGGA TCAGCAGCAA GCCCGGCAGT CCCAGCTTGC TCAGGACGAG 
CGGGTTTCAC GCTCCTACCT TGCCCTGGCA ACAGAGACCG TGGACATGTT CCATATCCTC 
ACGAAACAGG TCCAGAAGCC CTTCCTCAGA CCGGAACTTG GACCCCGATT GGCTGCAATG 
CTGAACTTTA ATCTCCAGCA ACTCTGTGGC CCCAAGTGCC GCGACCTGAA AGTTGAAAAC 
CCTGAGAAAT ATGGCTTTGA ACCAAAGAAG CTGTTGGACC AACTGACAGA CATTTACCTG 
CAGCTGGACT GTGCCCGGTT TGCCAAAGCC ATCGCTGATG ACCAGAGATC CTACAGCAAA 
GAGTTGTTTG AAGAAGTGAT CTCGAAGATG CGGAAGGCAG GGATCAAATC CACAATAGCT 
ATCGAGAAGT TTAAACTCCT CGCTGAGAAG GTGGAGGAGA TAGTGGCCAA GAACGCACGC 
GCAGAAATCG ACTACAGCGA CGCCCCCGAC GAGTTCAGAG ACCCACTGAT GGACACCCTG 
ATGACTGACC CTGTGCGGCT GCCCTCTGGC ACCATCATGG ACCGCTCCAT CATCCTGCGG 
CACTTGCTGA ACTCCCCCAC TGACCCCTTC AATCGACAGA TGCTGACAGA GAACATGCTG 
GAACCAGTGC CAGAACTGAA AGAACAGATT CACGCCTGGA TGAGAGAGAA ACAAAATAGT 
GATCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620583.1
CDS	717..4805
Translation	MEELSADEIRRRRLARLAGGQTSQPTTPLTSPQRENPLGPPIAASAPGPSQSLGLNVHNMTPATSPIGASGVAHRSQSSEGVSSLSSSPSNSLETQSQSLSRSQSMDIDGVSCEKSMSQVDVDSGIENMEVDENDRREKRSLSDKEPSSGSEVSEEQALQLVCKIFRVSWKDRDRDVIFLSSLSAQFKQNPKEVFSDFKDLIGQILMEVLMMSTQTRDENPFASLTATSQPIAAAARSPDRNLMLTTGSNPGTSPMFCGVGSFGASSFSSLYETSPAPSTSFWSSVPVMSPSFASPPARVAGQMVVPSISLSPHGGMASGTATGSQPSSPRYRPYTVTHPWGSSTSPTPRSPGISILSSSPSLPALASSPQAVPASSLRQRPSSLGPPSVAASPSAVSRRPSSLRISPSMYDNPFSFLFLALSGDSSDEDEEDDDDDDDGDDEGGGGGDEFSCVQFGSSLGASGGASTWDSYSDHFTIETCKETDMLNYLIECFDRVGIEEKKAPKMCSQPAVSQLLSNIRSQCISHTALVLQGSLTQPRSMQQPSFLVPYMLCRNLPYGFMQELVRTTHQDEEVFKQIFIPILQGLALAAKECSLDSDYFKYPLMALGELCETKFGKTHPVCNLVASLPLWLPKSLSPGSGRELQRLSYLGAFFSFSVFAEDDAKVVEKYFSGPAITLENTRVVSQSLQHYLELGRQELFKILHSILLNGETREAALGYMAAVVNANMKKAQMQTDDRLVSTDGFMLNFLWVLQQLSTKIKLETVDPTYIFHPRCRITLPNDETRVNATMEDVNDWLAELYGDQPPFSEPKFPTECFFLTLHAHHLSILPSCRRYIRRLRAIRELNRTVEDLKNNESQWKDSPLATRHREMLKRCKTQLKKLVRCKACADAGLLDESFLRRCLNFYGLLIQLLLRILDPAYPDITLPLNSDVPKVFAALPEFYVEDVAEFLFFIVQYSPQVLYEPCTQDIVMFLVVMLCNQNYIRNPYLVAKLVEVMFMTNPAVQPRTQKFFEMIENHPLSTKLLVPSLMKFYTDVEHTGATSEFYDKFTIRYHISTIFKSLWQNIAHHGTFMEEFNSGKQFVRYINMLINDTTFLLDESLESLKRIHEVQEEMKNKEQWDQLPRDQQQARQSQLAQDERVSRSYLALATETVDMFHILTKQVQKPFLRPELGPRLAAMLNFNLQQLCGPKCRDLKVENPEKYGFEPKKLLDQLTDIYLQLDCARFAKAIADDQRSYSKELFEEVISKMRKAGIKSTIAIEKFKLLAEKVEEIVAKNARAEIDYSDAPDEFRDPLMDTLMTDPVRLPSGTIMDRSIILRHLLNSPTDPFNRQMLTENMLEPVPELKEQIHAWMREKQNSDH

Target ORF information:

RefSeq Version	XM_005620526.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ubiquitination factor E4B (UBE4B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005620526.3

ORF Insert Sequence:

ATGGAGGAGC TGAGCGCTGA TGAGATCCGA CGGAGGCGCC TTGCACGACT TGCTGGTGGA 
CAGACCTCCC AGCCAACCAC CCCACTCACC TCGCCCCAGA GGGAGAACCC TCTGGGGCCT 
CCTATCGCAG CATCAGCCCC AGGCCCTTCC CAGAGCCTTG GTCTCAATGT CCACAACATG 
ACCCCAGCTA CCTCCCCTAT AGGTGCATCA GGAGTAGCTC ACCGAAGCCA GAGCAGTGAA 
GGAGTCAGTT CTCTCAGCAG CTCGCCCTCC AATAGCCTTG AAACCCAATC TCAGTCTCTC 
TCACGTTCCC AGAGCATGGA TATCGATGGG GTCTCTTGTG AAAAAAGCAT GTCCCAGGTG 
GATGTGGATT CAGGAATAGA AAATATGGAG GTTGATGAAA ACGATCGAAG AGAAAAGAGG 
AGCCTCAGCG ATAAGGAGCC TTCCTCGGGT TCTGAAGTAT CTGAAGAACA GGCCTTACAG 
CTGGTCTGTA AGATCTTTCG TGTCTCCTGG AAGGACCGGG ACAGAGATGT CATCTTTCTT 
TCTTCTCTTT CTGCACAATT TAAGCAGAAC CCAAAAGAAG TGTTCTCTGA TTTTAAGGAC 
TTGATTGGCC AGATTTTAAT GGAAGTGCTA ATGATGTCTA CGCAGACTAG AGATGAAAAT 
CCATTTGCCA GTCTGACAGC CACATCACAG CCAATTGCAG CAGCAGCTCG GTCACCAGAC 
AGGAATCTAA TGCTCACTAC TGGCTCTAAC CCAGGAACAA GCCCCATGTT CTGTGGTGTG 
GGTTCCTTTG GTGCCAGCTC TTTCTCTAGC CTCTATGAAA CTAGCCCAGC TCCCAGTACA 
AGTTTCTGGA GCTCGGTGCC AGTGATGAGT CCATCCTTTG CCTCCCCCCC TGCCCGTGTG 
GCTGGCCAGA TGGTTGTGCC CTCCATTTCC CTCAGCCCGC ATGGTGGCAT GGCCTCTGGA 
ACTGCCACAG GAAGCCAGCC CTCCTCTCCA CGGTATCGCC CCTACACTGT CACCCACCCG 
TGGGGGTCTT CGACTTCTCC TACCCCGCGA TCCCCAGGCA TCTCCATTCT GTCCAGCTCG 
CCCAGCCTCC CTGCCCTTGC TAGTAGCCCT CAAGCGGTTC CTGCCAGCTC TCTCAGACAG 
AGACCCAGCA GCCTGGGGCC CCCTTCAGTG GCCGCCTCAC CCAGTGCCGT GAGCAGACGT 
CCCTCATCCC TAAGGATCTC TCCTAGTATG TACGACAATC CTTTCTCCTT CCTCTTCCTC 
GCACTTTCTG GCGACAGTAG TGATGAAGAT GAAGAAGATG ATGATGATGA TGATGATGGT 
GATGATGAAG GTGGTGGTGG TGGTGATGAG TTTTCTTGTG TCCAGTTTGG GTCCAGTTTG 
GGAGCCTCTG GTGGGGCAAG TACTTGGGAT TCCTACAGCG ACCATTTCAC CATTGAGACC 
TGCAAGGAGA CAGACATGCT GAACTACCTC ATCGAGTGTT TTGACCGAGT GGGAATAGAG 
GAAAAAAAAG CACCAAAGAT GTGCAGCCAG CCAGCAGTCA GCCAGCTTCT CAGCAACATC 
CGCTCACAGT GCATATCCCA TACTGCGTTA GTCCTACAAG GTTCCCTCAC ACAACCAAGG 
TCCATGCAGC AGCCATCCTT CCTGGTGCCG TATATGCTCT GCAGGAATCT CCCATATGGC 
TTCATGCAGG AACTGGTGAG AACCACTCAC CAGGATGAAG AAGTGTTCAA GCAGATCTTT 
ATCCCCATTT TACAAGGCCT GGCTCTCGCT GCCAAAGAGT GCTCCCTCGA TAGTGACTAC 
TTTAAGTACC CCCTCATGGC TTTAGGTGAG CTTTGTGAAA CCAAGTTTGG GAAGACACAC 
CCCGTGTGCA ATTTGGTGGC TTCTTTGCCC TTGTGGTTGC CGAAATCCTT AAGCCCTGGC 
TCTGGGCGGG AGCTGCAGAG ACTCTCTTAT TTGGGAGCTT TCTTTAGCTT CTCAGTCTTT 
GCGGAAGATG ACGCTAAAGT GGTTGAAAAA TATTTCTCAG GGCCTGCCAT TACCCTGGAA 
AACACTCGCG TGGTCAGCCA ATCACTGCAG CATTACTTGG AGCTGGGAAG GCAAGAGCTT 
TTCAAGATTC TGCATAGTAT TCTGTTAAAC GGTGAGACCC GTGAGGCCGC TCTTGGTTAC 
ATGGCAGCTG TCGTCAATGC CAATATGAAG AAAGCACAGA TGCAGACAGA TGATAGATTG 
GTATCTACAG ATGGATTCAT GCTGAATTTC CTTTGGGTGC TGCAGCAGCT AAGTACAAAG 
ATCAAGTTAG AGACGGTGGA TCCCACGTAT ATATTCCACC CAAGGTGTCG GATTACTCTT 
CCTAATGACG AGACGAGAGT GAATGCTACA ATGGAGGATG TGAATGACTG GCTGGCTGAA 
CTTTATGGAG ACCAGCCTCC ATTTTCTGAG CCGAAATTTC CTACAGAATG CTTTTTTCTT 
ACCCTGCATG CCCACCACCT CTCCATTCTG CCAAGCTGCC GTCGCTATAT CCGTAGACTG 
CGGGCCATCC GGGAGCTCAA TAGAACTGTG GAAGATTTGA AAAATAATGA AAGTCAATGG 
AAAGATTCCC CACTGGCAAC CAGACACCGT GAAATGCTGA AGCGCTGCAA AACTCAGCTT 
AAGAAATTGG TGCGGTGCAA GGCCTGTGCT GATGCTGGTT TACTTGATGA GAGCTTTCTG 
AGAAGATGTC TGAATTTTTA TGGCCTTCTC ATCCAGCTGC TGCTCCGCAT CCTGGACCCC 
GCCTATCCCG ATATAACACT GCCTTTAAAC TCTGATGTCC CCAAGGTATT TGCAGCATTG 
CCTGAGTTTT ATGTAGAAGA TGTTGCTGAA TTTTTATTTT TTATTGTACA ATACTCTCCT 
CAGGTGCTGT ATGAGCCCTG TACTCAGGAT ATTGTGATGT TCCTCGTTGT GATGTTGTGT 
AACCAGAACT ACATCCGAAA TCCATATCTG GTGGCCAAAC TGGTAGAAGT CATGTTTATG 
ACCAACCCAG CTGTTCAGCC ACGAACCCAG AAGTTTTTTG AAATGATTGA AAACCATCCT 
CTCTCCACCA AGTTGTTGGT CCCTTCACTG ATGAAGTTTT ATACAGATGT TGAACACACC 
GGAGCCACCA GTGAGTTCTA CGACAAGTTC ACTATTCGCT ATCACATTAG CACCATTTTT 
AAAAGCCTTT GGCAAAACAT AGCTCACCAT GGCACCTTCA TGGAGGAGTT CAATTCGGGG 
AAGCAGTTTG TTCGCTATAT AAACATGCTG ATAAATGACA CGACCTTTTT ACTCGATGAA 
AGCCTGGAGT CTCTGAAGCG AATCCACGAA GTACAAGAAG AAATGAAGAA CAAAGAACAG 
TGGGACCAGC TGCCCCGGGA TCAGCAGCAA GCCCGGCAGT CCCAGCTTGC TCAGGACGAG 
CGGGTTTCAC GCTCCTACCT TGCCCTGGCA ACAGAGACCG TGGACATGTT CCATATCCTC 
ACGAAACAGG TCCAGAAGCC CTTCCTCAGA CCGGAACTTG GACCCCGATT GGCTGCAATG 
CTGAACTTTA ATCTCCAGCA ACTCTGTGGC CCCAAGTGCC GCGACCTGAA AGTTGAAAAC 
CCTGAGAAAT ATGGCTTTGA ACCAAAGAAG CTGTTGGACC AACTGACAGA CATTTACCTG 
CAGCTGGACT GTGCCCGGTT TGCCAAAGCC ATCGCTGATG ACCAGAGATC CTACAGCAAA 
GAGTTGTTTG AAGAAGTGAT CTCGAAGATG CGGAAGGCAG GGATCAAATC CACAATAGCT 
ATCGAGAAGT TTAAACTCCT CGCTGAGAAG GTGGAGGAGA TAGTGGCCAA GAACGCACGC 
GCAGAAATCG ACTACAGCGA CGCCCCCGAC GAGTTCAGAG ACCCACTGAT GGACACCCTG 
ATGACTGACC CTGTGCGGCT GCCCTCTGGC ACCATCATGG ACCGCTCCAT CATCCTGCGG 
CACTTGCTGA ACTCCCCCAC TGACCCCTTC AATCGACAGA TGCTGACAGA GAACATGCTG 
GAACCAGTGC CAGAACTGAA AGAACAGATT CACGCCTGGA TGAGAGAGAA ACAAAATAGT 
GATCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE4B ( XM_005620526.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005620583.1 Canis lupus familiaris ubiquitination factor E4B (UBE4B), transcript variant X1, mRNA.

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CloneID	OCe06876
Clone ID Related Accession (Same CDS sequence)	XM_005620526.3 , XM_005620526.2
Accession Version	XM_005620526.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	ubiquitin conjugation factor E4 B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726059.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005620526.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGAGCGCTGA TGAGATCCGA CGGAGGCGCC TTGCACGACT TGCTGGTGGA 
CAGACCTCCC AGCCAACCAC CCCACTCACC TCGCCCCAGA GGGAGAACCC TCTGGGGCCT 
CCTATCGCAG CATCAGCCCC AGGCCCTTCC CAGAGCCTTG GTCTCAATGT CCACAACATG 
ACCCCAGCTA CCTCCCCTAT AGGTGCATCA GGAGTAGCTC ACCGAAGCCA GAGCAGTGAA 
GGAGTCAGTT CTCTCAGCAG CTCGCCCTCC AATAGCCTTG AAACCCAATC TCAGTCTCTC 
TCACGTTCCC AGAGCATGGA TATCGATGGG GTCTCTTGTG AAAAAAGCAT GTCCCAGGTG 
GATGTGGATT CAGGAATAGA AAATATGGAG GTTGATGAAA ACGATCGAAG AGAAAAGAGG 
AGCCTCAGCG ATAAGGAGCC TTCCTCGGGT TCTGAAGTAT CTGAAGAACA GGCCTTACAG 
CTGGTCTGTA AGATCTTTCG TGTCTCCTGG AAGGACCGGG ACAGAGATGT CATCTTTCTT 
TCTTCTCTTT CTGCACAATT TAAGCAGAAC CCAAAAGAAG TGTTCTCTGA TTTTAAGGAC 
TTGATTGGCC AGATTTTAAT GGAAGTGCTA ATGATGTCTA CGCAGACTAG AGATGAAAAT 
CCATTTGCCA GTCTGACAGC CACATCACAG CCAATTGCAG CAGCAGCTCG GTCACCAGAC 
AGGAATCTAA TGCTCACTAC TGGCTCTAAC CCAGGAACAA GCCCCATGTT CTGTGGTGTG 
GGTTCCTTTG GTGCCAGCTC TTTCTCTAGC CTCTATGAAA CTAGCCCAGC TCCCAGTACA 
AGTTTCTGGA GCTCGGTGCC AGTGATGAGT CCATCCTTTG CCTCCCCCCC TGCCCGTGTG 
GCTGGCCAGA TGGTTGTGCC CTCCATTTCC CTCAGCCCGC ATGGTGGCAT GGCCTCTGGA 
ACTGCCACAG GAAGCCAGCC CTCCTCTCCA CGGTATCGCC CCTACACTGT CACCCACCCG 
TGGGGGTCTT CGACTTCTCC TACCCCGCGA TCCCCAGGCA TCTCCATTCT GTCCAGCTCG 
CCCAGCCTCC CTGCCCTTGC TAGTAGCCCT CAAGCGGTTC CTGCCAGCTC TCTCAGACAG 
AGACCCAGCA GCCTGGGGCC CCCTTCAGTG GCCGCCTCAC CCAGTGCCGT GAGCAGACGT 
CCCTCATCCC TAAGGATCTC TCCTAGTATG TACGACAATC CTTTCTCCTT CCTCTTCCTC 
GCACTTTCTG GCGACAGTAG TGATGAAGAT GAAGAAGATG ATGATGATGA TGATGATGGT 
GATGATGAAG GTGGTGGTGG TGGTGATGAG TTTTCTTGTG TCCAGTTTGG GTCCAGTTTG 
GGAGCCTCTG GTGGGGCAAG TACTTGGGAT TCCTACAGCG ACCATTTCAC CATTGAGACC 
TGCAAGGAGA CAGACATGCT GAACTACCTC ATCGAGTGTT TTGACCGAGT GGGAATAGAG 
GAAAAAAAAG CACCAAAGAT GTGCAGCCAG CCAGCAGTCA GCCAGCTTCT CAGCAACATC 
CGCTCACAGT GCATATCCCA TACTGCGTTA GTCCTACAAG GTTCCCTCAC ACAACCAAGG 
TCCATGCAGC AGCCATCCTT CCTGGTGCCG TATATGCTCT GCAGGAATCT CCCATATGGC 
TTCATGCAGG AACTGGTGAG AACCACTCAC CAGGATGAAG AAGTGTTCAA GCAGATCTTT 
ATCCCCATTT TACAAGGCCT GGCTCTCGCT GCCAAAGAGT GCTCCCTCGA TAGTGACTAC 
TTTAAGTACC CCCTCATGGC TTTAGGTGAG CTTTGTGAAA CCAAGTTTGG GAAGACACAC 
CCCGTGTGCA ATTTGGTGGC TTCTTTGCCC TTGTGGTTGC CGAAATCCTT AAGCCCTGGC 
TCTGGGCGGG AGCTGCAGAG ACTCTCTTAT TTGGGAGCTT TCTTTAGCTT CTCAGTCTTT 
GCGGAAGATG ACGCTAAAGT GGTTGAAAAA TATTTCTCAG GGCCTGCCAT TACCCTGGAA 
AACACTCGCG TGGTCAGCCA ATCACTGCAG CATTACTTGG AGCTGGGAAG GCAAGAGCTT 
TTCAAGATTC TGCATAGTAT TCTGTTAAAC GGTGAGACCC GTGAGGCCGC TCTTGGTTAC 
ATGGCAGCTG TCGTCAATGC CAATATGAAG AAAGCACAGA TGCAGACAGA TGATAGATTG 
GTATCTACAG ATGGATTCAT GCTGAATTTC CTTTGGGTGC TGCAGCAGCT AAGTACAAAG 
ATCAAGTTAG AGACGGTGGA TCCCACGTAT ATATTCCACC CAAGGTGTCG GATTACTCTT 
CCTAATGACG AGACGAGAGT GAATGCTACA ATGGAGGATG TGAATGACTG GCTGGCTGAA 
CTTTATGGAG ACCAGCCTCC ATTTTCTGAG CCGAAATTTC CTACAGAATG CTTTTTTCTT 
ACCCTGCATG CCCACCACCT CTCCATTCTG CCAAGCTGCC GTCGCTATAT CCGTAGACTG 
CGGGCCATCC GGGAGCTCAA TAGAACTGTG GAAGATTTGA AAAATAATGA AAGTCAATGG 
AAAGATTCCC CACTGGCAAC CAGACACCGT GAAATGCTGA AGCGCTGCAA AACTCAGCTT 
AAGAAATTGG TGCGGTGCAA GGCCTGTGCT GATGCTGGTT TACTTGATGA GAGCTTTCTG 
AGAAGATGTC TGAATTTTTA TGGCCTTCTC ATCCAGCTGC TGCTCCGCAT CCTGGACCCC 
GCCTATCCCG ATATAACACT GCCTTTAAAC TCTGATGTCC CCAAGGTATT TGCAGCATTG 
CCTGAGTTTT ATGTAGAAGA TGTTGCTGAA TTTTTATTTT TTATTGTACA ATACTCTCCT 
CAGGTGCTGT ATGAGCCCTG TACTCAGGAT ATTGTGATGT TCCTCGTTGT GATGTTGTGT 
AACCAGAACT ACATCCGAAA TCCATATCTG GTGGCCAAAC TGGTAGAAGT CATGTTTATG 
ACCAACCCAG CTGTTCAGCC ACGAACCCAG AAGTTTTTTG AAATGATTGA AAACCATCCT 
CTCTCCACCA AGTTGTTGGT CCCTTCACTG ATGAAGTTTT ATACAGATGT TGAACACACC 
GGAGCCACCA GTGAGTTCTA CGACAAGTTC ACTATTCGCT ATCACATTAG CACCATTTTT 
AAAAGCCTTT GGCAAAACAT AGCTCACCAT GGCACCTTCA TGGAGGAGTT CAATTCGGGG 
AAGCAGTTTG TTCGCTATAT AAACATGCTG ATAAATGACA CGACCTTTTT ACTCGATGAA 
AGCCTGGAGT CTCTGAAGCG AATCCACGAA GTACAAGAAG AAATGAAGAA CAAAGAACAG 
TGGGACCAGC TGCCCCGGGA TCAGCAGCAA GCCCGGCAGT CCCAGCTTGC TCAGGACGAG 
CGGGTTTCAC GCTCCTACCT TGCCCTGGCA ACAGAGACCG TGGACATGTT CCATATCCTC 
ACGAAACAGG TCCAGAAGCC CTTCCTCAGA CCGGAACTTG GACCCCGATT GGCTGCAATG 
CTGAACTTTA ATCTCCAGCA ACTCTGTGGC CCCAAGTGCC GCGACCTGAA AGTTGAAAAC 
CCTGAGAAAT ATGGCTTTGA ACCAAAGAAG CTGTTGGACC AACTGACAGA CATTTACCTG 
CAGCTGGACT GTGCCCGGTT TGCCAAAGCC ATCGCTGATG ACCAGAGATC CTACAGCAAA 
GAGTTGTTTG AAGAAGTGAT CTCGAAGATG CGGAAGGCAG GGATCAAATC CACAATAGCT 
ATCGAGAAGT TTAAACTCCT CGCTGAGAAG GTGGAGGAGA TAGTGGCCAA GAACGCACGC 
GCAGAAATCG ACTACAGCGA CGCCCCCGAC GAGTTCAGAG ACCCACTGAT GGACACCCTG 
ATGACTGACC CTGTGCGGCT GCCCTCTGGC ACCATCATGG ACCGCTCCAT CATCCTGCGG 
CACTTGCTGA ACTCCCCCAC TGACCCCTTC AATCGACAGA TGCTGACAGA GAACATGCTG 
GAACCAGTGC CAGAACTGAA AGAACAGATT CACGCCTGGA TGAGAGAGAA ACAAAATAGT 
GATCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620583.1
CDS	727..4815
Translation	MEELSADEIRRRRLARLAGGQTSQPTTPLTSPQRENPLGPPIAASAPGPSQSLGLNVHNMTPATSPIGASGVAHRSQSSEGVSSLSSSPSNSLETQSQSLSRSQSMDIDGVSCEKSMSQVDVDSGIENMEVDENDRREKRSLSDKEPSSGSEVSEEQALQLVCKIFRVSWKDRDRDVIFLSSLSAQFKQNPKEVFSDFKDLIGQILMEVLMMSTQTRDENPFASLTATSQPIAAAARSPDRNLMLTTGSNPGTSPMFCGVGSFGASSFSSLYETSPAPSTSFWSSVPVMSPSFASPPARVAGQMVVPSISLSPHGGMASGTATGSQPSSPRYRPYTVTHPWGSSTSPTPRSPGISILSSSPSLPALASSPQAVPASSLRQRPSSLGPPSVAASPSAVSRRPSSLRISPSMYDNPFSFLFLALSGDSSDEDEEDDDDDDDGDDEGGGGGDEFSCVQFGSSLGASGGASTWDSYSDHFTIETCKETDMLNYLIECFDRVGIEEKKAPKMCSQPAVSQLLSNIRSQCISHTALVLQGSLTQPRSMQQPSFLVPYMLCRNLPYGFMQELVRTTHQDEEVFKQIFIPILQGLALAAKECSLDSDYFKYPLMALGELCETKFGKTHPVCNLVASLPLWLPKSLSPGSGRELQRLSYLGAFFSFSVFAEDDAKVVEKYFSGPAITLENTRVVSQSLQHYLELGRQELFKILHSILLNGETREAALGYMAAVVNANMKKAQMQTDDRLVSTDGFMLNFLWVLQQLSTKIKLETVDPTYIFHPRCRITLPNDETRVNATMEDVNDWLAELYGDQPPFSEPKFPTECFFLTLHAHHLSILPSCRRYIRRLRAIRELNRTVEDLKNNESQWKDSPLATRHREMLKRCKTQLKKLVRCKACADAGLLDESFLRRCLNFYGLLIQLLLRILDPAYPDITLPLNSDVPKVFAALPEFYVEDVAEFLFFIVQYSPQVLYEPCTQDIVMFLVVMLCNQNYIRNPYLVAKLVEVMFMTNPAVQPRTQKFFEMIENHPLSTKLLVPSLMKFYTDVEHTGATSEFYDKFTIRYHISTIFKSLWQNIAHHGTFMEEFNSGKQFVRYINMLINDTTFLLDESLESLKRIHEVQEEMKNKEQWDQLPRDQQQARQSQLAQDERVSRSYLALATETVDMFHILTKQVQKPFLRPELGPRLAAMLNFNLQQLCGPKCRDLKVENPEKYGFEPKKLLDQLTDIYLQLDCARFAKAIADDQRSYSKELFEEVISKMRKAGIKSTIAIEKFKLLAEKVEEIVAKNARAEIDYSDAPDEFRDPLMDTLMTDPVRLPSGTIMDRSIILRHLLNSPTDPFNRQMLTENMLEPVPELKEQIHAWMREKQNSDH

Target ORF information:

RefSeq Version	XM_005620526.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ubiquitination factor E4B (UBE4B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005620526.2

ORF Insert Sequence:

ATGGAGGAGC TGAGCGCTGA TGAGATCCGA CGGAGGCGCC TTGCACGACT TGCTGGTGGA 
CAGACCTCCC AGCCAACCAC CCCACTCACC TCGCCCCAGA GGGAGAACCC TCTGGGGCCT 
CCTATCGCAG CATCAGCCCC AGGCCCTTCC CAGAGCCTTG GTCTCAATGT CCACAACATG 
ACCCCAGCTA CCTCCCCTAT AGGTGCATCA GGAGTAGCTC ACCGAAGCCA GAGCAGTGAA 
GGAGTCAGTT CTCTCAGCAG CTCGCCCTCC AATAGCCTTG AAACCCAATC TCAGTCTCTC 
TCACGTTCCC AGAGCATGGA TATCGATGGG GTCTCTTGTG AAAAAAGCAT GTCCCAGGTG 
GATGTGGATT CAGGAATAGA AAATATGGAG GTTGATGAAA ACGATCGAAG AGAAAAGAGG 
AGCCTCAGCG ATAAGGAGCC TTCCTCGGGT TCTGAAGTAT CTGAAGAACA GGCCTTACAG 
CTGGTCTGTA AGATCTTTCG TGTCTCCTGG AAGGACCGGG ACAGAGATGT CATCTTTCTT 
TCTTCTCTTT CTGCACAATT TAAGCAGAAC CCAAAAGAAG TGTTCTCTGA TTTTAAGGAC 
TTGATTGGCC AGATTTTAAT GGAAGTGCTA ATGATGTCTA CGCAGACTAG AGATGAAAAT 
CCATTTGCCA GTCTGACAGC CACATCACAG CCAATTGCAG CAGCAGCTCG GTCACCAGAC 
AGGAATCTAA TGCTCACTAC TGGCTCTAAC CCAGGAACAA GCCCCATGTT CTGTGGTGTG 
GGTTCCTTTG GTGCCAGCTC TTTCTCTAGC CTCTATGAAA CTAGCCCAGC TCCCAGTACA 
AGTTTCTGGA GCTCGGTGCC AGTGATGAGT CCATCCTTTG CCTCCCCCCC TGCCCGTGTG 
GCTGGCCAGA TGGTTGTGCC CTCCATTTCC CTCAGCCCGC ATGGTGGCAT GGCCTCTGGA 
ACTGCCACAG GAAGCCAGCC CTCCTCTCCA CGGTATCGCC CCTACACTGT CACCCACCCG 
TGGGGGTCTT CGACTTCTCC TACCCCGCGA TCCCCAGGCA TCTCCATTCT GTCCAGCTCG 
CCCAGCCTCC CTGCCCTTGC TAGTAGCCCT CAAGCGGTTC CTGCCAGCTC TCTCAGACAG 
AGACCCAGCA GCCTGGGGCC CCCTTCAGTG GCCGCCTCAC CCAGTGCCGT GAGCAGACGT 
CCCTCATCCC TAAGGATCTC TCCTAGTATG TACGACAATC CTTTCTCCTT CCTCTTCCTC 
GCACTTTCTG GCGACAGTAG TGATGAAGAT GAAGAAGATG ATGATGATGA TGATGATGGT 
GATGATGAAG GTGGTGGTGG TGGTGATGAG TTTTCTTGTG TCCAGTTTGG GTCCAGTTTG 
GGAGCCTCTG GTGGGGCAAG TACTTGGGAT TCCTACAGCG ACCATTTCAC CATTGAGACC 
TGCAAGGAGA CAGACATGCT GAACTACCTC ATCGAGTGTT TTGACCGAGT GGGAATAGAG 
GAAAAAAAAG CACCAAAGAT GTGCAGCCAG CCAGCAGTCA GCCAGCTTCT CAGCAACATC 
CGCTCACAGT GCATATCCCA TACTGCGTTA GTCCTACAAG GTTCCCTCAC ACAACCAAGG 
TCCATGCAGC AGCCATCCTT CCTGGTGCCG TATATGCTCT GCAGGAATCT CCCATATGGC 
TTCATGCAGG AACTGGTGAG AACCACTCAC CAGGATGAAG AAGTGTTCAA GCAGATCTTT 
ATCCCCATTT TACAAGGCCT GGCTCTCGCT GCCAAAGAGT GCTCCCTCGA TAGTGACTAC 
TTTAAGTACC CCCTCATGGC TTTAGGTGAG CTTTGTGAAA CCAAGTTTGG GAAGACACAC 
CCCGTGTGCA ATTTGGTGGC TTCTTTGCCC TTGTGGTTGC CGAAATCCTT AAGCCCTGGC 
TCTGGGCGGG AGCTGCAGAG ACTCTCTTAT TTGGGAGCTT TCTTTAGCTT CTCAGTCTTT 
GCGGAAGATG ACGCTAAAGT GGTTGAAAAA TATTTCTCAG GGCCTGCCAT TACCCTGGAA 
AACACTCGCG TGGTCAGCCA ATCACTGCAG CATTACTTGG AGCTGGGAAG GCAAGAGCTT 
TTCAAGATTC TGCATAGTAT TCTGTTAAAC GGTGAGACCC GTGAGGCCGC TCTTGGTTAC 
ATGGCAGCTG TCGTCAATGC CAATATGAAG AAAGCACAGA TGCAGACAGA TGATAGATTG 
GTATCTACAG ATGGATTCAT GCTGAATTTC CTTTGGGTGC TGCAGCAGCT AAGTACAAAG 
ATCAAGTTAG AGACGGTGGA TCCCACGTAT ATATTCCACC CAAGGTGTCG GATTACTCTT 
CCTAATGACG AGACGAGAGT GAATGCTACA ATGGAGGATG TGAATGACTG GCTGGCTGAA 
CTTTATGGAG ACCAGCCTCC ATTTTCTGAG CCGAAATTTC CTACAGAATG CTTTTTTCTT 
ACCCTGCATG CCCACCACCT CTCCATTCTG CCAAGCTGCC GTCGCTATAT CCGTAGACTG 
CGGGCCATCC GGGAGCTCAA TAGAACTGTG GAAGATTTGA AAAATAATGA AAGTCAATGG 
AAAGATTCCC CACTGGCAAC CAGACACCGT GAAATGCTGA AGCGCTGCAA AACTCAGCTT 
AAGAAATTGG TGCGGTGCAA GGCCTGTGCT GATGCTGGTT TACTTGATGA GAGCTTTCTG 
AGAAGATGTC TGAATTTTTA TGGCCTTCTC ATCCAGCTGC TGCTCCGCAT CCTGGACCCC 
GCCTATCCCG ATATAACACT GCCTTTAAAC TCTGATGTCC CCAAGGTATT TGCAGCATTG 
CCTGAGTTTT ATGTAGAAGA TGTTGCTGAA TTTTTATTTT TTATTGTACA ATACTCTCCT 
CAGGTGCTGT ATGAGCCCTG TACTCAGGAT ATTGTGATGT TCCTCGTTGT GATGTTGTGT 
AACCAGAACT ACATCCGAAA TCCATATCTG GTGGCCAAAC TGGTAGAAGT CATGTTTATG 
ACCAACCCAG CTGTTCAGCC ACGAACCCAG AAGTTTTTTG AAATGATTGA AAACCATCCT 
CTCTCCACCA AGTTGTTGGT CCCTTCACTG ATGAAGTTTT ATACAGATGT TGAACACACC 
GGAGCCACCA GTGAGTTCTA CGACAAGTTC ACTATTCGCT ATCACATTAG CACCATTTTT 
AAAAGCCTTT GGCAAAACAT AGCTCACCAT GGCACCTTCA TGGAGGAGTT CAATTCGGGG 
AAGCAGTTTG TTCGCTATAT AAACATGCTG ATAAATGACA CGACCTTTTT ACTCGATGAA 
AGCCTGGAGT CTCTGAAGCG AATCCACGAA GTACAAGAAG AAATGAAGAA CAAAGAACAG 
TGGGACCAGC TGCCCCGGGA TCAGCAGCAA GCCCGGCAGT CCCAGCTTGC TCAGGACGAG 
CGGGTTTCAC GCTCCTACCT TGCCCTGGCA ACAGAGACCG TGGACATGTT CCATATCCTC 
ACGAAACAGG TCCAGAAGCC CTTCCTCAGA CCGGAACTTG GACCCCGATT GGCTGCAATG 
CTGAACTTTA ATCTCCAGCA ACTCTGTGGC CCCAAGTGCC GCGACCTGAA AGTTGAAAAC 
CCTGAGAAAT ATGGCTTTGA ACCAAAGAAG CTGTTGGACC AACTGACAGA CATTTACCTG 
CAGCTGGACT GTGCCCGGTT TGCCAAAGCC ATCGCTGATG ACCAGAGATC CTACAGCAAA 
GAGTTGTTTG AAGAAGTGAT CTCGAAGATG CGGAAGGCAG GGATCAAATC CACAATAGCT 
ATCGAGAAGT TTAAACTCCT CGCTGAGAAG GTGGAGGAGA TAGTGGCCAA GAACGCACGC 
GCAGAAATCG ACTACAGCGA CGCCCCCGAC GAGTTCAGAG ACCCACTGAT GGACACCCTG 
ATGACTGACC CTGTGCGGCT GCCCTCTGGC ACCATCATGG ACCGCTCCAT CATCCTGCGG 
CACTTGCTGA ACTCCCCCAC TGACCCCTTC AATCGACAGA TGCTGACAGA GAACATGCTG 
GAACCAGTGC CAGAACTGAA AGAACAGATT CACGCCTGGA TGAGAGAGAA ACAAAATAGT 
GATCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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UBE4B cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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