VCL cDNA ORF clone, Canis lupus familiaris(dog)

The following VCL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VCL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe05580 XM_536395.6
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Canis lupus familiaris vinculin (VCL), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
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OCe05580 XM_536395.5 Canis lupus familiaris vinculin (VCL), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe05580
    Clone ID Related Accession (Same CDS sequence) XM_536395.5 , XM_536395.6
    Accession Version XM_536395.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3513bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product vinculin
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726056.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_536395.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 4% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCGGTGT TTCATACGCG CACGATCGAG AGCATCTTGG AGCCGGTGGC GCAGCAGATC 
    TCGCACCTGG TGATCATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC
    ACCGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TTGTCCGGGT TGGAAAAGAG
    ACTGTTCAAA CCACCGAAGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG
    GTTGAGAATG CTTGCACCAA GCTTGTCCAG GCAGCCCAGA TGCTTCAGTC AGATCCTTAC
    TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC CGGCACATCA
    GACCTGCTCC TCACCTTCGA TGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA
    ATTTTGGAAT ATCTTACAGT GGCAGAAGTG GTGGAAACTA TGGAAGATTT GGTCACCTAC
    ACAAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAGA TGATTGATGA GAGGCAGCAG
    GAACTGACTC ACCAGGAGCA CCGAGTAATG TTGGTGAACT CCATGAACAC CGTGAAAGAG
    TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA TTCAAAAAAC
    CAAGGAATAG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAGAAGAT GAGTGCTGAA
    ATTAATGAGA TCATTCGTGT ATTACAACTC ACTTCTTGGG ATGAAGATGC CTGGGCCAGC
    AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCC
    AAAGGTTGGC TCCGTGATCC CAGTGCCTCT CCAGGGGATG CTGGTGAGCA GGCCATCAGA
    CAGATCTTAG ATGAAGCTGG AAAAGTCGGT GAACTCTGCG CAGGCAAAGA GCGCAGGGAG
    ATCCTGGGAA CCTGTAAAAT GCTGGGGCAG ATGACTGATC AAGTGGCTGA ACTCCGAGCC
    AGAGGACAAG GAGCCTCACC AGTGGCCATG CAGAAAGCCC AGCAGGTGTC TCAGGGTCTG
    GATGTGCTCA CAGCAAAAGT GGAAAATGCA GCCCGCAAGC TGGAAGCTAT GACCAACTCA
    AAGCAGAGCA TTGCCAAGAA GATTGATGCT GCTCAGAATT GGCTTGCAGA TCCAAATGGT
    GGACCAGAAG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTAGGAA AATAGCAGAG
    TTGTGTGATG ATCCTAAAGA GAGGGATGAC ATTCTACGTT CTCTTGGGGA GATATCTGCT
    CTGACTTCTA AATTAGCAGA TCTGCGGAGA CAGGGCAAAG GAGATTCCCC AGAGGCCCGA
    GCATTGGCCA AACAGGTGGC CACAGCCCTG CAGAACTTGC AGACCAAAAC TAATAGGGCT
    GTGGCCAATA GCAGGCCCGC CAAGGCAGCT GTCCACCTTG AAGGCAAGAT TGAGCAAGCA
    CAGCGGTGGA TTGATAATCC CACAGTGGAT GACCGGGGTG TTGGTCAGGC TGCCATCCGG
    GGGCTTGTGG CCGAAGGGCA TCGTCTGGCT AATGTCATGA TGGGGCCATA TCGCCAAGAT
    CTTCTTGCCA AGTGTGACCG GGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTTGCTGCC
    AGAGGGGAAG GAGAGAGTCC TCAGGCGAGA GCACTTGCCT CTCAGCTCCA AGACTCCTTA
    AAGGATCTGA AAGCCCGGAT GCAGGAGGCG ATGACTCAGG AGGTGTCAGA TGTTTTCAGC
    GATACCACAA CTCCCATCAA GCTGTTGGCA GTGGCGGCCA CTGCACCTCC TGATGCTCCC
    AGTAGGGAGG AAGTATTTGA TGAGAGGGCA GCTAACTTTG AGCACCATTC AGGAAGGCTT
    GGTGCCACAG CGGAGAAGGC GGCTGCTGTT GGAACTGCTA ATAAATCCAC CGTAGAAGGC
    ATTCAGGCAT CAGTAAAGAC GGCCCGAGAA CTCACACCCC AGGTGGTCTC AGCTGCTCGC
    ATTTTACTTA GGAATCCTGG AAATCAAGCT GCTTACGAAC ACTTTGAGAC CATGAAGAAC
    CAGTGGATCG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCTAT TGATACCAAA
    TCTCTGTTGG ATGCTTCCGA AGAAGCAATT AAAAAAGACC TGGACAAGTG TAAAGTAGCC
    ATGGCCAACA TTCAGCCACA GATGTTGGTC GCCGGGGCGA CGAGCATCGC TCGCCGGGCC
    AACCGTATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC TAAGTTCCGT
    GAGGCTGTGA AAGCTGCCTC CGATGAATTG AGCAAAACCA TCTCCCCAAT GGTGATGGAT
    GCAAAGGCTG TGGCTGGAAA CATTTCCGAC CCTGGCCTGC AAAAGAGCTT CCTGGACTCA
    GGATATCGGA TCCTGGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAGCCT
    GACTTCCCGC CTCCTCCTCC AGATCTTGAA CAGCTCCGTC TGGCAGATGA GCTTGCTCCT
    CCCAAACCAC CTCTGCCTGA GGGTGAGGTC CCTCCGCCCA GGCCCCCACC ACCAGAGGAG
    AAGGATGAAG AGTTCCCTGA GCAGAAAGCC GGGGAGGTGA TTAACCAGCC AATGATGATG
    GCTGCCAGGC AGCTCCATGA TGAGGCTCGC AAATGGTCCA GCAAGCCGGG TAACCGAGCC
    GCTGAGGTGG GTATAGGCGT TGTAGCTGAG GCAGATGCGG CCGATGCTGT CGGGTTCCCT
    GTCCCCCCTG ACATGGAAGA CGATTTCGAA CCTGAGCTGC TGTTAATGCC ATCCAATCAG
    CCGGTCAACC AGCCCATTCT GGCCGCGGCT CAGTCATTGC ATCGGGAAGC TACCAAGTGG
    TCTAGTAAGG GCAATGACAT CATTGCAGCA GCCAAACGCA TGGCTCTGCT GATGGCTGAG
    ATGTCTCGGC TGGTAAGAGG GGGCAGTGGT ACCAAGCGGG CACTGATTCA GTGTGCCAAG
    GACATTGCCA AGGCCTCAGA TGAGGTGACG CGGTTGGCCA AGGAGGTTGC CAAGCAGTGC
    ACAGATAAGC GGATTAGAAC CAACCTCTTA CAGGTATGCG AGCGAATCCC AACCATAAGC
    ACCCAACTCA AGATCCTGTC CACGGTGAAG GCCACCATGC TGGGTCGCAC CAACATCAGT
    GATGAGGAGT CTGAGCAGGC CACAGAGATG CTGGTTCACA ATGCCCAGAA CCTCATGCAG
    TCAGTGAAGG AGCCTGTTCG AGAAGCAGAA GCTGCTTCAA TCAAAATTCG AACGGGGGGG
    GGTTTTCCCC GGGGGGGGTG CAAAACCAAA TTTTGCTCTC AGGAGGAAAT CGCAGAATCA
    CATTCGTGTG CTTCTGTCTT TACCTTGCCC CTGAGCAACG TCTGTGCTAG GGAAGGTTCC
    TGTCCTGTAG CCCGCCTCGG CCTGCCAAGG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_536395.3
    CDS196..3708
    Translation

    Target ORF information:

    RefSeq Version XM_536395.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris vinculin (VCL), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_536395.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCGGTGT TTCATACGCG CACGATCGAG AGCATCTTGG AGCCGGTGGC GCAGCAGATC 
    TCGCACCTGG TGATCATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC
    ACCGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TTGTCCGGGT TGGAAAAGAG
    ACTGTTCAAA CCACCGAAGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG
    GTTGAGAATG CTTGCACCAA GCTTGTCCAG GCAGCCCAGA TGCTTCAGTC AGATCCTTAC
    TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC CGGCACATCA
    GACCTGCTCC TCACCTTCGA TGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA
    ATTTTGGAAT ATCTTACAGT GGCAGAAGTG GTGGAAACTA TGGAAGATTT GGTCACCTAC
    ACAAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAGA TGATTGATGA GAGGCAGCAG
    GAACTGACTC ACCAGGAGCA CCGAGTAATG TTGGTGAACT CCATGAACAC CGTGAAAGAG
    TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA TTCAAAAAAC
    CAAGGAATAG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAGAAGAT GAGTGCTGAA
    ATTAATGAGA TCATTCGTGT ATTACAACTC ACTTCTTGGG ATGAAGATGC CTGGGCCAGC
    AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCC
    AAAGGTTGGC TCCGTGATCC CAGTGCCTCT CCAGGGGATG CTGGTGAGCA GGCCATCAGA
    CAGATCTTAG ATGAAGCTGG AAAAGTCGGT GAACTCTGCG CAGGCAAAGA GCGCAGGGAG
    ATCCTGGGAA CCTGTAAAAT GCTGGGGCAG ATGACTGATC AAGTGGCTGA ACTCCGAGCC
    AGAGGACAAG GAGCCTCACC AGTGGCCATG CAGAAAGCCC AGCAGGTGTC TCAGGGTCTG
    GATGTGCTCA CAGCAAAAGT GGAAAATGCA GCCCGCAAGC TGGAAGCTAT GACCAACTCA
    AAGCAGAGCA TTGCCAAGAA GATTGATGCT GCTCAGAATT GGCTTGCAGA TCCAAATGGT
    GGACCAGAAG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTAGGAA AATAGCAGAG
    TTGTGTGATG ATCCTAAAGA GAGGGATGAC ATTCTACGTT CTCTTGGGGA GATATCTGCT
    CTGACTTCTA AATTAGCAGA TCTGCGGAGA CAGGGCAAAG GAGATTCCCC AGAGGCCCGA
    GCATTGGCCA AACAGGTGGC CACAGCCCTG CAGAACTTGC AGACCAAAAC TAATAGGGCT
    GTGGCCAATA GCAGGCCCGC CAAGGCAGCT GTCCACCTTG AAGGCAAGAT TGAGCAAGCA
    CAGCGGTGGA TTGATAATCC CACAGTGGAT GACCGGGGTG TTGGTCAGGC TGCCATCCGG
    GGGCTTGTGG CCGAAGGGCA TCGTCTGGCT AATGTCATGA TGGGGCCATA TCGCCAAGAT
    CTTCTTGCCA AGTGTGACCG GGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTTGCTGCC
    AGAGGGGAAG GAGAGAGTCC TCAGGCGAGA GCACTTGCCT CTCAGCTCCA AGACTCCTTA
    AAGGATCTGA AAGCCCGGAT GCAGGAGGCG ATGACTCAGG AGGTGTCAGA TGTTTTCAGC
    GATACCACAA CTCCCATCAA GCTGTTGGCA GTGGCGGCCA CTGCACCTCC TGATGCTCCC
    AGTAGGGAGG AAGTATTTGA TGAGAGGGCA GCTAACTTTG AGCACCATTC AGGAAGGCTT
    GGTGCCACAG CGGAGAAGGC GGCTGCTGTT GGAACTGCTA ATAAATCCAC CGTAGAAGGC
    ATTCAGGCAT CAGTAAAGAC GGCCCGAGAA CTCACACCCC AGGTGGTCTC AGCTGCTCGC
    ATTTTACTTA GGAATCCTGG AAATCAAGCT GCTTACGAAC ACTTTGAGAC CATGAAGAAC
    CAGTGGATCG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCTAT TGATACCAAA
    TCTCTGTTGG ATGCTTCCGA AGAAGCAATT AAAAAAGACC TGGACAAGTG TAAAGTAGCC
    ATGGCCAACA TTCAGCCACA GATGTTGGTC GCCGGGGCGA CGAGCATCGC TCGCCGGGCC
    AACCGTATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC TAAGTTCCGT
    GAGGCTGTGA AAGCTGCCTC CGATGAATTG AGCAAAACCA TCTCCCCAAT GGTGATGGAT
    GCAAAGGCTG TGGCTGGAAA CATTTCCGAC CCTGGCCTGC AAAAGAGCTT CCTGGACTCA
    GGATATCGGA TCCTGGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAGCCT
    GACTTCCCGC CTCCTCCTCC AGATCTTGAA CAGCTCCGTC TGGCAGATGA GCTTGCTCCT
    CCCAAACCAC CTCTGCCTGA GGGTGAGGTC CCTCCGCCCA GGCCCCCACC ACCAGAGGAG
    AAGGATGAAG AGTTCCCTGA GCAGAAAGCC GGGGAGGTGA TTAACCAGCC AATGATGATG
    GCTGCCAGGC AGCTCCATGA TGAGGCTCGC AAATGGTCCA GCAAGCCGGG TAACCGAGCC
    GCTGAGGTGG GTATAGGCGT TGTAGCTGAG GCAGATGCGG CCGATGCTGT CGGGTTCCCT
    GTCCCCCCTG ACATGGAAGA CGATTTCGAA CCTGAGCTGC TGTTAATGCC ATCCAATCAG
    CCGGTCAACC AGCCCATTCT GGCCGCGGCT CAGTCATTGC ATCGGGAAGC TACCAAGTGG
    TCTAGTAAGG GCAATGACAT CATTGCAGCA GCCAAACGCA TGGCTCTGCT GATGGCTGAG
    ATGTCTCGGC TGGTAAGAGG GGGCAGTGGT ACCAAGCGGG CACTGATTCA GTGTGCCAAG
    GACATTGCCA AGGCCTCAGA TGAGGTGACG CGGTTGGCCA AGGAGGTTGC CAAGCAGTGC
    ACAGATAAGC GGATTAGAAC CAACCTCTTA CAGGTATGCG AGCGAATCCC AACCATAAGC
    ACCCAACTCA AGATCCTGTC CACGGTGAAG GCCACCATGC TGGGTCGCAC CAACATCAGT
    GATGAGGAGT CTGAGCAGGC CACAGAGATG CTGGTTCACA ATGCCCAGAA CCTCATGCAG
    TCAGTGAAGG AGCCTGTTCG AGAAGCAGAA GCTGCTTCAA TCAAAATTCG AACGGGGGGG
    GGTTTTCCCC GGGGGGGGTG CAAAACCAAA TTTTGCTCTC AGGAGGAAAT CGCAGAATCA
    CATTCGTGTG CTTCTGTCTT TACCTTGCCC CTGAGCAACG TCTGTGCTAG GGAAGGTTCC
    TGTCCTGTAG CCCGCCTCGG CCTGCCAAGG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe05580
    Clone ID Related Accession (Same CDS sequence) XM_536395.5 , XM_536395.6
    Accession Version XM_536395.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3513bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product vinculin
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_536395.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 4% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCGGTGT TTCATACGCG CACGATCGAG AGCATCTTGG AGCCGGTGGC GCAGCAGATC 
    TCGCACCTGG TGATCATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC
    ACCGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TTGTCCGGGT TGGAAAAGAG
    ACTGTTCAAA CCACCGAAGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG
    GTTGAGAATG CTTGCACCAA GCTTGTCCAG GCAGCCCAGA TGCTTCAGTC AGATCCTTAC
    TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC CGGCACATCA
    GACCTGCTCC TCACCTTCGA TGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA
    ATTTTGGAAT ATCTTACAGT GGCAGAAGTG GTGGAAACTA TGGAAGATTT GGTCACCTAC
    ACAAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAGA TGATTGATGA GAGGCAGCAG
    GAACTGACTC ACCAGGAGCA CCGAGTAATG TTGGTGAACT CCATGAACAC CGTGAAAGAG
    TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA TTCAAAAAAC
    CAAGGAATAG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAGAAGAT GAGTGCTGAA
    ATTAATGAGA TCATTCGTGT ATTACAACTC ACTTCTTGGG ATGAAGATGC CTGGGCCAGC
    AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCC
    AAAGGTTGGC TCCGTGATCC CAGTGCCTCT CCAGGGGATG CTGGTGAGCA GGCCATCAGA
    CAGATCTTAG ATGAAGCTGG AAAAGTCGGT GAACTCTGCG CAGGCAAAGA GCGCAGGGAG
    ATCCTGGGAA CCTGTAAAAT GCTGGGGCAG ATGACTGATC AAGTGGCTGA ACTCCGAGCC
    AGAGGACAAG GAGCCTCACC AGTGGCCATG CAGAAAGCCC AGCAGGTGTC TCAGGGTCTG
    GATGTGCTCA CAGCAAAAGT GGAAAATGCA GCCCGCAAGC TGGAAGCTAT GACCAACTCA
    AAGCAGAGCA TTGCCAAGAA GATTGATGCT GCTCAGAATT GGCTTGCAGA TCCAAATGGT
    GGACCAGAAG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTAGGAA AATAGCAGAG
    TTGTGTGATG ATCCTAAAGA GAGGGATGAC ATTCTACGTT CTCTTGGGGA GATATCTGCT
    CTGACTTCTA AATTAGCAGA TCTGCGGAGA CAGGGCAAAG GAGATTCCCC AGAGGCCCGA
    GCATTGGCCA AACAGGTGGC CACAGCCCTG CAGAACTTGC AGACCAAAAC TAATAGGGCT
    GTGGCCAATA GCAGGCCCGC CAAGGCAGCT GTCCACCTTG AAGGCAAGAT TGAGCAAGCA
    CAGCGGTGGA TTGATAATCC CACAGTGGAT GACCGGGGTG TTGGTCAGGC TGCCATCCGG
    GGGCTTGTGG CCGAAGGGCA TCGTCTGGCT AATGTCATGA TGGGGCCATA TCGCCAAGAT
    CTTCTTGCCA AGTGTGACCG GGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTTGCTGCC
    AGAGGGGAAG GAGAGAGTCC TCAGGCGAGA GCACTTGCCT CTCAGCTCCA AGACTCCTTA
    AAGGATCTGA AAGCCCGGAT GCAGGAGGCG ATGACTCAGG AGGTGTCAGA TGTTTTCAGC
    GATACCACAA CTCCCATCAA GCTGTTGGCA GTGGCGGCCA CTGCACCTCC TGATGCTCCC
    AGTAGGGAGG AAGTATTTGA TGAGAGGGCA GCTAACTTTG AGCACCATTC AGGAAGGCTT
    GGTGCCACAG CGGAGAAGGC GGCTGCTGTT GGAACTGCTA ATAAATCCAC CGTAGAAGGC
    ATTCAGGCAT CAGTAAAGAC GGCCCGAGAA CTCACACCCC AGGTGGTCTC AGCTGCTCGC
    ATTTTACTTA GGAATCCTGG AAATCAAGCT GCTTACGAAC ACTTTGAGAC CATGAAGAAC
    CAGTGGATCG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCTAT TGATACCAAA
    TCTCTGTTGG ATGCTTCCGA AGAAGCAATT AAAAAAGACC TGGACAAGTG TAAAGTAGCC
    ATGGCCAACA TTCAGCCACA GATGTTGGTC GCCGGGGCGA CGAGCATCGC TCGCCGGGCC
    AACCGTATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC TAAGTTCCGT
    GAGGCTGTGA AAGCTGCCTC CGATGAATTG AGCAAAACCA TCTCCCCAAT GGTGATGGAT
    GCAAAGGCTG TGGCTGGAAA CATTTCCGAC CCTGGCCTGC AAAAGAGCTT CCTGGACTCA
    GGATATCGGA TCCTGGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAGCCT
    GACTTCCCGC CTCCTCCTCC AGATCTTGAA CAGCTCCGTC TGGCAGATGA GCTTGCTCCT
    CCCAAACCAC CTCTGCCTGA GGGTGAGGTC CCTCCGCCCA GGCCCCCACC ACCAGAGGAG
    AAGGATGAAG AGTTCCCTGA GCAGAAAGCC GGGGAGGTGA TTAACCAGCC AATGATGATG
    GCTGCCAGGC AGCTCCATGA TGAGGCTCGC AAATGGTCCA GCAAGCCGGG TAACCGAGCC
    GCTGAGGTGG GTATAGGCGT TGTAGCTGAG GCAGATGCGG CCGATGCTGT CGGGTTCCCT
    GTCCCCCCTG ACATGGAAGA CGATTTCGAA CCTGAGCTGC TGTTAATGCC ATCCAATCAG
    CCGGTCAACC AGCCCATTCT GGCCGCGGCT CAGTCATTGC ATCGGGAAGC TACCAAGTGG
    TCTAGTAAGG GCAATGACAT CATTGCAGCA GCCAAACGCA TGGCTCTGCT GATGGCTGAG
    ATGTCTCGGC TGGTAAGAGG GGGCAGTGGT ACCAAGCGGG CACTGATTCA GTGTGCCAAG
    GACATTGCCA AGGCCTCAGA TGAGGTGACG CGGTTGGCCA AGGAGGTTGC CAAGCAGTGC
    ACAGATAAGC GGATTAGAAC CAACCTCTTA CAGGTATGCG AGCGAATCCC AACCATAAGC
    ACCCAACTCA AGATCCTGTC CACGGTGAAG GCCACCATGC TGGGTCGCAC CAACATCAGT
    GATGAGGAGT CTGAGCAGGC CACAGAGATG CTGGTTCACA ATGCCCAGAA CCTCATGCAG
    TCAGTGAAGG AGCCTGTTCG AGAAGCAGAA GCTGCTTCAA TCAAAATTCG AACGGGGGGG
    GGTTTTCCCC GGGGGGGGTG CAAAACCAAA TTTTGCTCTC AGGAGGAAAT CGCAGAATCA
    CATTCGTGTG CTTCTGTCTT TACCTTGCCC CTGAGCAACG TCTGTGCTAG GGAAGGTTCC
    TGTCCTGTAG CCCGCCTCGG CCTGCCAAGG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_536395.3
    CDS111..3623
    Translation

    Target ORF information:

    RefSeq Version XM_536395.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris vinculin (VCL), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_536395.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCGGTGT TTCATACGCG CACGATCGAG AGCATCTTGG AGCCGGTGGC GCAGCAGATC 
    TCGCACCTGG TGATCATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC
    ACCGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TTGTCCGGGT TGGAAAAGAG
    ACTGTTCAAA CCACCGAAGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG
    GTTGAGAATG CTTGCACCAA GCTTGTCCAG GCAGCCCAGA TGCTTCAGTC AGATCCTTAC
    TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC CGGCACATCA
    GACCTGCTCC TCACCTTCGA TGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA
    ATTTTGGAAT ATCTTACAGT GGCAGAAGTG GTGGAAACTA TGGAAGATTT GGTCACCTAC
    ACAAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAGA TGATTGATGA GAGGCAGCAG
    GAACTGACTC ACCAGGAGCA CCGAGTAATG TTGGTGAACT CCATGAACAC CGTGAAAGAG
    TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA TTCAAAAAAC
    CAAGGAATAG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAGAAGAT GAGTGCTGAA
    ATTAATGAGA TCATTCGTGT ATTACAACTC ACTTCTTGGG ATGAAGATGC CTGGGCCAGC
    AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCC
    AAAGGTTGGC TCCGTGATCC CAGTGCCTCT CCAGGGGATG CTGGTGAGCA GGCCATCAGA
    CAGATCTTAG ATGAAGCTGG AAAAGTCGGT GAACTCTGCG CAGGCAAAGA GCGCAGGGAG
    ATCCTGGGAA CCTGTAAAAT GCTGGGGCAG ATGACTGATC AAGTGGCTGA ACTCCGAGCC
    AGAGGACAAG GAGCCTCACC AGTGGCCATG CAGAAAGCCC AGCAGGTGTC TCAGGGTCTG
    GATGTGCTCA CAGCAAAAGT GGAAAATGCA GCCCGCAAGC TGGAAGCTAT GACCAACTCA
    AAGCAGAGCA TTGCCAAGAA GATTGATGCT GCTCAGAATT GGCTTGCAGA TCCAAATGGT
    GGACCAGAAG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTAGGAA AATAGCAGAG
    TTGTGTGATG ATCCTAAAGA GAGGGATGAC ATTCTACGTT CTCTTGGGGA GATATCTGCT
    CTGACTTCTA AATTAGCAGA TCTGCGGAGA CAGGGCAAAG GAGATTCCCC AGAGGCCCGA
    GCATTGGCCA AACAGGTGGC CACAGCCCTG CAGAACTTGC AGACCAAAAC TAATAGGGCT
    GTGGCCAATA GCAGGCCCGC CAAGGCAGCT GTCCACCTTG AAGGCAAGAT TGAGCAAGCA
    CAGCGGTGGA TTGATAATCC CACAGTGGAT GACCGGGGTG TTGGTCAGGC TGCCATCCGG
    GGGCTTGTGG CCGAAGGGCA TCGTCTGGCT AATGTCATGA TGGGGCCATA TCGCCAAGAT
    CTTCTTGCCA AGTGTGACCG GGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTTGCTGCC
    AGAGGGGAAG GAGAGAGTCC TCAGGCGAGA GCACTTGCCT CTCAGCTCCA AGACTCCTTA
    AAGGATCTGA AAGCCCGGAT GCAGGAGGCG ATGACTCAGG AGGTGTCAGA TGTTTTCAGC
    GATACCACAA CTCCCATCAA GCTGTTGGCA GTGGCGGCCA CTGCACCTCC TGATGCTCCC
    AGTAGGGAGG AAGTATTTGA TGAGAGGGCA GCTAACTTTG AGCACCATTC AGGAAGGCTT
    GGTGCCACAG CGGAGAAGGC GGCTGCTGTT GGAACTGCTA ATAAATCCAC CGTAGAAGGC
    ATTCAGGCAT CAGTAAAGAC GGCCCGAGAA CTCACACCCC AGGTGGTCTC AGCTGCTCGC
    ATTTTACTTA GGAATCCTGG AAATCAAGCT GCTTACGAAC ACTTTGAGAC CATGAAGAAC
    CAGTGGATCG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCTAT TGATACCAAA
    TCTCTGTTGG ATGCTTCCGA AGAAGCAATT AAAAAAGACC TGGACAAGTG TAAAGTAGCC
    ATGGCCAACA TTCAGCCACA GATGTTGGTC GCCGGGGCGA CGAGCATCGC TCGCCGGGCC
    AACCGTATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC TAAGTTCCGT
    GAGGCTGTGA AAGCTGCCTC CGATGAATTG AGCAAAACCA TCTCCCCAAT GGTGATGGAT
    GCAAAGGCTG TGGCTGGAAA CATTTCCGAC CCTGGCCTGC AAAAGAGCTT CCTGGACTCA
    GGATATCGGA TCCTGGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAGCCT
    GACTTCCCGC CTCCTCCTCC AGATCTTGAA CAGCTCCGTC TGGCAGATGA GCTTGCTCCT
    CCCAAACCAC CTCTGCCTGA GGGTGAGGTC CCTCCGCCCA GGCCCCCACC ACCAGAGGAG
    AAGGATGAAG AGTTCCCTGA GCAGAAAGCC GGGGAGGTGA TTAACCAGCC AATGATGATG
    GCTGCCAGGC AGCTCCATGA TGAGGCTCGC AAATGGTCCA GCAAGCCGGG TAACCGAGCC
    GCTGAGGTGG GTATAGGCGT TGTAGCTGAG GCAGATGCGG CCGATGCTGT CGGGTTCCCT
    GTCCCCCCTG ACATGGAAGA CGATTTCGAA CCTGAGCTGC TGTTAATGCC ATCCAATCAG
    CCGGTCAACC AGCCCATTCT GGCCGCGGCT CAGTCATTGC ATCGGGAAGC TACCAAGTGG
    TCTAGTAAGG GCAATGACAT CATTGCAGCA GCCAAACGCA TGGCTCTGCT GATGGCTGAG
    ATGTCTCGGC TGGTAAGAGG GGGCAGTGGT ACCAAGCGGG CACTGATTCA GTGTGCCAAG
    GACATTGCCA AGGCCTCAGA TGAGGTGACG CGGTTGGCCA AGGAGGTTGC CAAGCAGTGC
    ACAGATAAGC GGATTAGAAC CAACCTCTTA CAGGTATGCG AGCGAATCCC AACCATAAGC
    ACCCAACTCA AGATCCTGTC CACGGTGAAG GCCACCATGC TGGGTCGCAC CAACATCAGT
    GATGAGGAGT CTGAGCAGGC CACAGAGATG CTGGTTCACA ATGCCCAGAA CCTCATGCAG
    TCAGTGAAGG AGCCTGTTCG AGAAGCAGAA GCTGCTTCAA TCAAAATTCG AACGGGGGGG
    GGTTTTCCCC GGGGGGGGTG CAAAACCAAA TTTTGCTCTC AGGAGGAAAT CGCAGAATCA
    CATTCGTGTG CTTCTGTCTT TACCTTGCCC CTGAGCAACG TCTGTGCTAG GGAAGGTTCC
    TGTCCTGTAG CCCGCCTCGG CCTGCCAAGG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.