CAST gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_014112202.2	XP_013967677.1	calpastatin isoform X14
XM_005618081.3	XP_005618138.2	calpastatin isoform X18
XM_005618082.3	XP_005618139.2	calpastatin isoform X19
XM_022416706.1	XP_022272414.1	calpastatin isoform X20
XM_014112203.1	XP_013967678.1	calpastatin isoform X12
XM_014112204.1	XP_013967679.1	calpastatin isoform X13
XM_005618082.2	XP_005618139.2	calpastatin isoform X15
XM_005618076.2	XP_005618133.2	calpastatin isoform X5
XM_014112197.1	XP_013967672.1	calpastatin isoform X6
XM_014112199.1	XP_013967674.1	calpastatin isoform X8
XM_014112200.1	XP_013967675.1	calpastatin isoform X9
XM_014112202.1	XP_013967677.1	calpastatin isoform X11
XM_005618083.3	XP_005618140.1	calpastatin isoform X1
XM_005618074.3	XP_005618131.2	calpastatin isoform X2
XM_014112195.2	XP_013967670.1	calpastatin isoform X3
XM_014112197.2	XP_013967672.1	calpastatin isoform X6
XM_022416703.1	XP_022272411.1	calpastatin isoform X7
XM_022416704.1	XP_022272412.1	calpastatin isoform X8
XM_014112198.2	XP_013967673.1	calpastatin isoform X9
XM_014112199.2	XP_013967674.1	calpastatin isoform X10
XM_022416705.1	XP_022272413.1	calpastatin isoform X13
XM_014112201.1	XP_013967676.1	calpastatin isoform X10
XM_005618083.2	XP_005618140.1	calpastatin isoform X1
XM_843528.5	XP_848621.3	calpastatin isoform X4
XM_014112200.2	XP_013967675.1	calpastatin isoform X11
XM_014112204.2	XP_013967679.1	calpastatin isoform X16
XM_843528.4	XP_848621.3	calpastatin isoform X4
XM_005618081.2	XP_005618138.2	calpastatin isoform X14
XM_005618084.2	XP_005618141.2	calpastatin isoform X16
XM_014112195.1	XP_013967670.1	calpastatin isoform X3
XM_014112198.1	XP_013967673.1	calpastatin isoform X7
XM_005618076.3	XP_005618133.2	calpastatin isoform X5
XM_014112201.2	XP_013967676.1	calpastatin isoform X12
XM_014112203.2	XP_013967678.1	calpastatin isoform X15
XM_005618074.2	XP_005618131.2	calpastatin isoform X2
XM_005618084.3	XP_005618141.2	calpastatin isoform X17

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe287111	XM_022416706.1 Latest version!	Canis lupus familiaris calpastatin (CAST), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCe35583	XM_005618076.3 Latest version!	Canis lupus familiaris calpastatin (CAST), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCe35592	XM_005618081.3 Latest version!	Canis lupus familiaris calpastatin (CAST), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCe35593	XM_005618082.3 Latest version!	Canis lupus familiaris calpastatin (CAST), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCe35594	XM_005618084.3 Latest version!	Canis lupus familiaris calpastatin (CAST), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCe03968	XM_005618083.3 Latest version!	Canis lupus familiaris calpastatin (CAST), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe03969	XM_843528.5 Latest version!	Canis lupus familiaris calpastatin (CAST), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe287108	XM_022416703.1 Latest version!	Canis lupus familiaris calpastatin (CAST), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe287109	XM_022416704.1 Latest version!	Canis lupus familiaris calpastatin (CAST), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe287110	XM_022416705.1 Latest version!	Canis lupus familiaris calpastatin (CAST), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe35581	XM_005618074.3 Latest version!	Canis lupus familiaris calpastatin (CAST), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe35582	XM_014112195.2 Latest version!	Canis lupus familiaris calpastatin (CAST), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe35584	XM_014112197.2 Latest version!	Canis lupus familiaris calpastatin (CAST), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe35585	XM_014112198.2 Latest version!	Canis lupus familiaris calpastatin (CAST), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe35586	XM_014112199.2 Latest version!	Canis lupus familiaris calpastatin (CAST), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe35587	XM_014112200.2 Latest version!	Canis lupus familiaris calpastatin (CAST), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe35588	XM_014112201.2 Latest version!	Canis lupus familiaris calpastatin (CAST), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe35589	XM_014112202.2 Latest version!	Canis lupus familiaris calpastatin (CAST), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe35590	XM_014112203.2 Latest version!	Canis lupus familiaris calpastatin (CAST), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe35591	XM_014112204.2 Latest version!	Canis lupus familiaris calpastatin (CAST), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
View Old Accession Versions >>
OCe35593	XM_005618082.2	Canis lupus familiaris calpastatin (CAST), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCe35589	XM_014112202.1	Canis lupus familiaris calpastatin (CAST), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe35584	XM_014112197.1	Canis lupus familiaris calpastatin (CAST), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe35586	XM_014112199.1	Canis lupus familiaris calpastatin (CAST), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe03968	XM_005618083.2	Canis lupus familiaris calpastatin (CAST), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe35587	XM_014112200.1	Canis lupus familiaris calpastatin (CAST), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe35591	XM_014112204.1	Canis lupus familiaris calpastatin (CAST), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe03969	XM_843528.4	Canis lupus familiaris calpastatin (CAST), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe35592	XM_005618081.2	Canis lupus familiaris calpastatin (CAST), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCe35582	XM_014112195.1	Canis lupus familiaris calpastatin (CAST), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe35585	XM_014112198.1	Canis lupus familiaris calpastatin (CAST), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe35583	XM_005618076.2	Canis lupus familiaris calpastatin (CAST), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCe35588	XM_014112201.1	Canis lupus familiaris calpastatin (CAST), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe35590	XM_014112203.1	Canis lupus familiaris calpastatin (CAST), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe35581	XM_005618074.2	Canis lupus familiaris calpastatin (CAST), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe35594	XM_005618084.2	Canis lupus familiaris calpastatin (CAST), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
Hide Old Accession Versions >>

CAST ( XM_022416706.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022272414.1 Canis lupus familiaris calpastatin (CAST), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OCe287111
Clone ID Related Accession (Same CDS sequence)	XM_022416706.1
Accession Version	XM_022416706.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	calpastatin isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCCA CAGAAACCAA GGCTGCAAAA ACAGAACCTG AAAAGAAGTC ACAATCAACT 
AAGCCAAAAA GCCTACCCAA GCATGCATCA GATATGGGAA GCAAGCATGT TCATAGTGAA 
AAAGCAGCTT CTAGATCAAG TGAGCAGCTG ACATCAGAGA AATCAACAAA ACCAAAGACT 
AAAGCACAGG ATTCCGTTTC TGCCGGTGGG GAGAGTGTTG TTGCAGGTGC AGCTGCAGCT 
GCAGCATCTG CCACAGCAGC TAACAAGCAG AAAAAAGAAA AGAAATCACT AACATCGGCT 
GTACCAGTTG AATCCAAACC GGGTAAACCA CCTGGAAAGT CAGACATGGA TGCTGCTTTG 
GATGACTTAG TAGACACTCT AGGAAAACCT GAAGAAACTA AAGAAGATAA TGTACCATAT 
ACTGGACCTG AAATTTCAGA TCCGATGACT TCCACCTACA TAGAGGAATT AGGTAAAAGA 
GAAGTCACAC TTCCTCCAAA GTACAGGGAA CTTTTGGCAA AAAAAGAAGG TATCACAGTG 
CCTCCTCCAG ACTCTTCGAA ACCCATGGGG CCCAATGATG CCATAGATGC TTTGTCATCT 
GACTTCACCT GCAGTTCACC TACCGATGCT GGAAAGAAAA CTAAGGAAGA GAAATCTACA 
GGAGAAGAGA TTTTAAAAGC CCAGCCAGCC AGGGTAACCA AAAGTGCTGC TTCACCCCAA 
GAGAAAAAAA GAAAGGTGGA GGAGCAGGAC GCAATGAGTG ATCAAGCACT GGAGGCTCTT 
TCAGCTTCTT TGGGCAGCCG GAAGCCAGAA CCCGAGCTGG ACCTCAGTTC CATTAAGGAA 
GTCGACGAGG CAAAAGCCAA AGAAGAGAAA CTAAAGAAGT GTGGTGAAGA TGAGGAAACT 
GTCCCATCAG AGTACAGATT AAAACCAGCT ACGGATAAAG ATGGAAAACC TCTATTGCCA 
GAGCCTGAAG AAAAATCCAA GCCCCTGAGT GAATCAGAAC TCATTGATGA GCTTTCAGAA 
GATTTTAACC AGCCTAAAAG TAAAGGAGAA CAGTCTAAGC CAACTGAAAA AACAGAAGAA 
TCTCAGGCTG CCCCCCTAAC TCCTGTGAGC GAGGCTGTGC CTCGGACCTC CATGTGTAGC 
ATACAGTCGG CACCGCCCAA GCCACCTACC TTGGAAGGCA TGGTGCCAGA TGATGCTGTA 
GAAGCCTTGG CTGGTAGCCT GGGGAAGAAG GAAGCAGATC CAGGAGATGG AAAGCCTGTG 
GAAGATAAAG TCAAGGAGAA AGCCAAGGAA GAGGATCGTG AAAAACTTGG TGAAAAAGAA 
GAAACAATTC CTCCTGATTA TAGATTAGAA GAGGTCAAGG ATAAAGATGG AAAACCACTC 
CTGCCAAAAG TGCCCAAGGA ATCGCTTTTA CCCATGAGTG AAGACTTCCT TCTTGATGCT 
TTGTCCAAGG ACTTTGCTGG TTCCTCAAAC ACTTCACCTC TTGAATTTGA AGAGGCTAAC 
CTTCCTGCTG TCATCTCTGA AGTGGTTTCC CAAACCCCAG CTCCAACCAC GCACTCTGCT 
GGTCTACCCC CTGATACTGT GCAAAGTGAC AACAAAGAAC TGGACGATGC TTTGGACCAA 
CTTTCTGACA GTCTGGGACA AAGGCAGCCT GATCCAGATG AGAACAAGCC GATAGAGGAT 
AAAGTCAAGG AAAGAGCTAA AGCTGAACAC AGAGACAAGC TGGGAGAAAG AGACGACACC 
ATCCCCCCTG AATATAGACA CCTCTTGGAT AACGATGAGG GTAAACCAGG AAAGCCACCT 
GCCAAGAAAA CCAAGGACTC AAAGAAGCCT GAAGATGACA AAGACCCCAT CGATGCCCTG 
TCAGGCGACT TCGACAGCTG TCCTGCACCC ACAGAAGCCT CAGAGAACAC AGCAGAGGAC 
AAAGCCAAGA GAACTGCTCC CAGCTCCAAA ACACCGAAGA ATAGGGGTAA AGCAAAGGAT 
TCTGCAAAGG CAAAGCAGGA AACTTCCAAG CCCAAAGCTG ATGAAAAAAA TACAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022272414.1
CDS	173..2212
Translation	MNPTETKAAKTEPEKKSQSTKPKSLPKHASDMGSKHVHSEKAASRSSEQLTSEKSTKPKTKAQDSVSAGGESVVAGAAAAAASATAANKQKKEKKSLTSAVPVESKPGKPPGKSDMDAALDDLVDTLGKPEETKEDNVPYTGPEISDPMTSTYIEELGKREVTLPPKYRELLAKKEGITVPPPDSSKPMGPNDAIDALSSDFTCSSPTDAGKKTKEEKSTGEEILKAQPARVTKSAASPQEKKRKVEEQDAMSDQALEALSASLGSRKPEPELDLSSIKEVDEAKAKEEKLKKCGEDEETVPSEYRLKPATDKDGKPLLPEPEEKSKPLSESELIDELSEDFNQPKSKGEQSKPTEKTEESQAAPLTPVSEAVPRTSMCSIQSAPPKPPTLEGMVPDDAVEALAGSLGKKEADPGDGKPVEDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKVPKESLLPMSEDFLLDALSKDFAGSSNTSPLEFEEANLPAVISEVVSQTPAPTTHSAGLPPDTVQSDNKELDDALDQLSDSLGQRQPDPDENKPIEDKVKERAKAEHRDKLGERDDTIPPEYRHLLDNDEGKPGKPPAKKTKDSKKPEDDKDPIDALSGDFDSCPAPTEASENTAEDKAKRTAPSSKTPKNRGKAKDSAKAKQETSKPKADEKNTS

Target ORF information:

RefSeq Version	XM_022416706.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calpastatin (CAST), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022416706.1

ORF Insert Sequence:

ATGAATCCCA CAGAAACCAA GGCTGCAAAA ACAGAACCTG AAAAGAAGTC ACAATCAACT 
AAGCCAAAAA GCCTACCCAA GCATGCATCA GATATGGGAA GCAAGCATGT TCATAGTGAA 
AAAGCAGCTT CTAGATCAAG TGAGCAGCTG ACATCAGAGA AATCAACAAA ACCAAAGACT 
AAAGCACAGG ATTCCGTTTC TGCCGGTGGG GAGAGTGTTG TTGCAGGTGC AGCTGCAGCT 
GCAGCATCTG CCACAGCAGC TAACAAGCAG AAAAAAGAAA AGAAATCACT AACATCGGCT 
GTACCAGTTG AATCCAAACC GGGTAAACCA CCTGGAAAGT CAGACATGGA TGCTGCTTTG 
GATGACTTAG TAGACACTCT AGGAAAACCT GAAGAAACTA AAGAAGATAA TGTACCATAT 
ACTGGACCTG AAATTTCAGA TCCGATGACT TCCACCTACA TAGAGGAATT AGGTAAAAGA 
GAAGTCACAC TTCCTCCAAA GTACAGGGAA CTTTTGGCAA AAAAAGAAGG TATCACAGTG 
CCTCCTCCAG ACTCTTCGAA ACCCATGGGG CCCAATGATG CCATAGATGC TTTGTCATCT 
GACTTCACCT GCAGTTCACC TACCGATGCT GGAAAGAAAA CTAAGGAAGA GAAATCTACA 
GGAGAAGAGA TTTTAAAAGC CCAGCCAGCC AGGGTAACCA AAAGTGCTGC TTCACCCCAA 
GAGAAAAAAA GAAAGGTGGA GGAGCAGGAC GCAATGAGTG ATCAAGCACT GGAGGCTCTT 
TCAGCTTCTT TGGGCAGCCG GAAGCCAGAA CCCGAGCTGG ACCTCAGTTC CATTAAGGAA 
GTCGACGAGG CAAAAGCCAA AGAAGAGAAA CTAAAGAAGT GTGGTGAAGA TGAGGAAACT 
GTCCCATCAG AGTACAGATT AAAACCAGCT ACGGATAAAG ATGGAAAACC TCTATTGCCA 
GAGCCTGAAG AAAAATCCAA GCCCCTGAGT GAATCAGAAC TCATTGATGA GCTTTCAGAA 
GATTTTAACC AGCCTAAAAG TAAAGGAGAA CAGTCTAAGC CAACTGAAAA AACAGAAGAA 
TCTCAGGCTG CCCCCCTAAC TCCTGTGAGC GAGGCTGTGC CTCGGACCTC CATGTGTAGC 
ATACAGTCGG CACCGCCCAA GCCACCTACC TTGGAAGGCA TGGTGCCAGA TGATGCTGTA 
GAAGCCTTGG CTGGTAGCCT GGGGAAGAAG GAAGCAGATC CAGGAGATGG AAAGCCTGTG 
GAAGATAAAG TCAAGGAGAA AGCCAAGGAA GAGGATCGTG AAAAACTTGG TGAAAAAGAA 
GAAACAATTC CTCCTGATTA TAGATTAGAA GAGGTCAAGG ATAAAGATGG AAAACCACTC 
CTGCCAAAAG TGCCCAAGGA ATCGCTTTTA CCCATGAGTG AAGACTTCCT TCTTGATGCT 
TTGTCCAAGG ACTTTGCTGG TTCCTCAAAC ACTTCACCTC TTGAATTTGA AGAGGCTAAC 
CTTCCTGCTG TCATCTCTGA AGTGGTTTCC CAAACCCCAG CTCCAACCAC GCACTCTGCT 
GGTCTACCCC CTGATACTGT GCAAAGTGAC AACAAAGAAC TGGACGATGC TTTGGACCAA 
CTTTCTGACA GTCTGGGACA AAGGCAGCCT GATCCAGATG AGAACAAGCC GATAGAGGAT 
AAAGTCAAGG AAAGAGCTAA AGCTGAACAC AGAGACAAGC TGGGAGAAAG AGACGACACC 
ATCCCCCCTG AATATAGACA CCTCTTGGAT AACGATGAGG GTAAACCAGG AAAGCCACCT 
GCCAAGAAAA CCAAGGACTC AAAGAAGCCT GAAGATGACA AAGACCCCAT CGATGCCCTG 
TCAGGCGACT TCGACAGCTG TCCTGCACCC ACAGAAGCCT CAGAGAACAC AGCAGAGGAC 
AAAGCCAAGA GAACTGCTCC CAGCTCCAAA ACACCGAAGA ATAGGGGTAA AGCAAAGGAT 
TCTGCAAAGG CAAAGCAGGA AACTTCCAAG CCCAAAGCTG ATGAAAAAAA TACAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_005618076.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005618133.2 Canis lupus familiaris calpastatin (CAST), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe35583
Clone ID Related Accession (Same CDS sequence)	XM_005618076.2 , XM_005618076.3
Accession Version	XM_005618076.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	calpastatin isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726052.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005618076.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTCG CAAGCTGGTG GTACAAGACG CATGTCAGCG AAAAAACAAG TGGATCACCT 
TCCAAGTCAG GAGAAAAGAA AGGAGCAGAT GAGAAAAAAG CAAGAAGCCT TGAGAGCAGT 
CAACCTTCCA GAACCCATGC TGGTGGAACA GCCCCAGCCA CCAAGGCGTC AGCCTCTTCT 
GCTTCTACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCTAT TCCAGTCAGC 
AAACAGACGG GAGGACAGCA TTCTCCTAAC AAGAAAAGAC ACAAAAAACA GGCTGCAAAA 
ACAGAACCTG AAAAGAAGTC ACAATCAACT AAGCCATCTG TGGTCCATGA AAAAAAAACC 
CAAGAAGTAA AGCCAAAGGA ACACAAAGAG CCAAAAAGCC TACCCAAGCA TGCATCAGAT 
ATGGGAAGCA AGCATGTTCA TAGTGAAAAA GCAGCTTCTA GATCAAGTGA GCAGCTGACA 
TCAGAGAAAT CAACAAAACC AAAGACTAAA GCACAGGATT CCGTTTCTGC CGGTGGGGAG 
AGTGTTGTTG CAGGTGCAGC TGCAGCTGCA GCATCTGCCA CAGCAGCTAA CAAGCAGAAA 
AAAGAAAAGA AATCACTAAC ATCGGCTGTA CCAGTTGAAT CCAAACCGGG TAAACCACCT 
GGAAAGTCAG ACATGGATGC TGCTTTGGAT GACTTAGTAG ACACTCTAGG AAAACCTGAA 
GAAACTAAAG AAGATAATGT ACCATATACT GGACCTGAAA TTTCAGATCC GATGACTTCC 
ACCTACATAG AGGAATTAGG TAAAAGAGAA GTCACACTTC CTCCAAAGTA CAGGGAACTT 
TTGGCAAAAA AAGAAGGTAT CACAGTGCCT CCTCCAGACT CTTCGAAACC CATGGGGCCC 
AATGATGCCA TAGATGCTTT GTCATCTGAC TTCACCTGCA GTTCACCTAC CGATGCTGGA 
AAGAAAACTA AGGAAGAGAA ATCTACAGGA GAAGAGATTT TAAAAGCCCA GCCAGCCAGG 
GTAACCAAAA GTGCTGCTTC ACCCCAAGAG AAAAAAAGAA AGGTGGAGGA GCAGGACGCA 
ATGAGTGATC AAGCACTGGA GGCTCTTTCA GCTTCTTTGG GCAGCCGGAA GCCAGAACCC 
GAGCTGGACC TCAGTTCCAT TAAGGAAGTC GACGAGGCAA AAGCCAAAGA AGAGAAACTA 
AAGAAGTGTG GTGAAGATGA GGAAACTGTC CCATCAGAGT ACAGATTAAA ACCAGCTACG 
GATAAAGATG GAAAACCTCT ATTGCCAGAG CCTGAAGAAA AATCCAAGCC CCTGAGTGAA 
TCAGAACTCA TTGATGAGCT TTCAGAAGAT TTTAACCAGC CTAAAAGTAA AGGAGAACAG 
TCTAAGCCAA CTGAAAAAAC AGAAGAATCT CAGGCTGCCC CCCTAACTCC TGTGAGCGAG 
GCTGTGCCTC GGACCTCCAT GTGTAGCATA CAGTCGGCAC CGCCCAAGCC ACCTACCTTG 
GAAGGCATGG TGCCAGATGA TGCTGTAGAA GCCTTGGCTG GTAGCCTGGG GAAGAAGGAA 
GCAGATCCAG GAGATGGAAA GCCTGTGGAA GATAAAGTCA AGGAGAAAGC CAAGGAAGAG 
GATCGTGAAA AACTTGGTGA AAAAGAAGAA ACAATTCCTC CTGATTATAG ATTAGAAGAG 
GTCAAGGATA AAGATGGAAA ACCACTCCTG CCAAAAGTGC CCAAGGAATC GCTTTTACCC 
ATGAGTGAAG ACTTCCTTCT TGATGCTTTG TCCAAGGACT TTGCTGGTTC CTCAAACACT 
TCACCTCTTG AATTTGAAGA GGCTAACCTT CCTGCTGTCA TCTCTGAAGT GGTTTCCCAA 
ACCCCAGCTC CAACCACGCA CTCTGCTGGT CTACCCCCTG ATACTGTGCA AAGTGACAAC 
AAAGAACTGG ACGATGCTTT GGACCAACTT TCTGACAGTC TGGGACAAAG GCAGCCTGAT 
CCAGATGAGA ACAAGCCGAT AGAGGATAAA GTCAAGGAAA GAGCTAAAGC TGAACACAGA 
GACAAGCTGG GAGAAAGAGA CGACACCATC CCCCCTGAAT ATAGACACCT CTTGGATAAC 
GATGAGGGTA AACCAGGAAA GCCACCTGCC AAGAAAACCA AGGACTCAAA GAAGCCTGAA 
GATGACAAAG ACCCCATCGA TGCCCTGTCA GGCGACTTCG ACAGCTGTCC TGCACCCACA 
GAAGCCTCAG AGAACACAGC AGAGGACAAA GCCAAGAGAA CTGCTCCCAG CTCCAAAACA 
CCGAAGAATA GGGGTAAAGC AAAGGATTCT GCAAAGGCAA AGCAGGAAAC TTCCAAGCCC 
AAAGCTGATG AAAAAAATAC AAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005618133.2
CDS	144..2510
Translation	MAFASWWYKTHVSEKTSGSPSKSGEKKGADEKKARSLESSQPSRTHAGGTAPATKASASSASTSKSSSMNPTETKAIPVSKQTGGQHSPNKKRHKKQAAKTEPEKKSQSTKPSVVHEKKTQEVKPKEHKEPKSLPKHASDMGSKHVHSEKAASRSSEQLTSEKSTKPKTKAQDSVSAGGESVVAGAAAAAASATAANKQKKEKKSLTSAVPVESKPGKPPGKSDMDAALDDLVDTLGKPEETKEDNVPYTGPEISDPMTSTYIEELGKREVTLPPKYRELLAKKEGITVPPPDSSKPMGPNDAIDALSSDFTCSSPTDAGKKTKEEKSTGEEILKAQPARVTKSAASPQEKKRKVEEQDAMSDQALEALSASLGSRKPEPELDLSSIKEVDEAKAKEEKLKKCGEDEETVPSEYRLKPATDKDGKPLLPEPEEKSKPLSESELIDELSEDFNQPKSKGEQSKPTEKTEESQAAPLTPVSEAVPRTSMCSIQSAPPKPPTLEGMVPDDAVEALAGSLGKKEADPGDGKPVEDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKVPKESLLPMSEDFLLDALSKDFAGSSNTSPLEFEEANLPAVISEVVSQTPAPTTHSAGLPPDTVQSDNKELDDALDQLSDSLGQRQPDPDENKPIEDKVKERAKAEHRDKLGERDDTIPPEYRHLLDNDEGKPGKPPAKKTKDSKKPEDDKDPIDALSGDFDSCPAPTEASENTAEDKAKRTAPSSKTPKNRGKAKDSAKAKQETSKPKADEKNTS

Target ORF information:

RefSeq Version	XM_005618076.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calpastatin (CAST), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005618076.2

ORF Insert Sequence:

ATGGCATTCG CAAGCTGGTG GTACAAGACG CATGTCAGCG AAAAAACAAG TGGATCACCT 
TCCAAGTCAG GAGAAAAGAA AGGAGCAGAT GAGAAAAAAG CAAGAAGCCT TGAGAGCAGT 
CAACCTTCCA GAACCCATGC TGGTGGAACA GCCCCAGCCA CCAAGGCGTC AGCCTCTTCT 
GCTTCTACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCTAT TCCAGTCAGC 
AAACAGACGG GAGGACAGCA TTCTCCTAAC AAGAAAAGAC ACAAAAAACA GGCTGCAAAA 
ACAGAACCTG AAAAGAAGTC ACAATCAACT AAGCCATCTG TGGTCCATGA AAAAAAAACC 
CAAGAAGTAA AGCCAAAGGA ACACAAAGAG CCAAAAAGCC TACCCAAGCA TGCATCAGAT 
ATGGGAAGCA AGCATGTTCA TAGTGAAAAA GCAGCTTCTA GATCAAGTGA GCAGCTGACA 
TCAGAGAAAT CAACAAAACC AAAGACTAAA GCACAGGATT CCGTTTCTGC CGGTGGGGAG 
AGTGTTGTTG CAGGTGCAGC TGCAGCTGCA GCATCTGCCA CAGCAGCTAA CAAGCAGAAA 
AAAGAAAAGA AATCACTAAC ATCGGCTGTA CCAGTTGAAT CCAAACCGGG TAAACCACCT 
GGAAAGTCAG ACATGGATGC TGCTTTGGAT GACTTAGTAG ACACTCTAGG AAAACCTGAA 
GAAACTAAAG AAGATAATGT ACCATATACT GGACCTGAAA TTTCAGATCC GATGACTTCC 
ACCTACATAG AGGAATTAGG TAAAAGAGAA GTCACACTTC CTCCAAAGTA CAGGGAACTT 
TTGGCAAAAA AAGAAGGTAT CACAGTGCCT CCTCCAGACT CTTCGAAACC CATGGGGCCC 
AATGATGCCA TAGATGCTTT GTCATCTGAC TTCACCTGCA GTTCACCTAC CGATGCTGGA 
AAGAAAACTA AGGAAGAGAA ATCTACAGGA GAAGAGATTT TAAAAGCCCA GCCAGCCAGG 
GTAACCAAAA GTGCTGCTTC ACCCCAAGAG AAAAAAAGAA AGGTGGAGGA GCAGGACGCA 
ATGAGTGATC AAGCACTGGA GGCTCTTTCA GCTTCTTTGG GCAGCCGGAA GCCAGAACCC 
GAGCTGGACC TCAGTTCCAT TAAGGAAGTC GACGAGGCAA AAGCCAAAGA AGAGAAACTA 
AAGAAGTGTG GTGAAGATGA GGAAACTGTC CCATCAGAGT ACAGATTAAA ACCAGCTACG 
GATAAAGATG GAAAACCTCT ATTGCCAGAG CCTGAAGAAA AATCCAAGCC CCTGAGTGAA 
TCAGAACTCA TTGATGAGCT TTCAGAAGAT TTTAACCAGC CTAAAAGTAA AGGAGAACAG 
TCTAAGCCAA CTGAAAAAAC AGAAGAATCT CAGGCTGCCC CCCTAACTCC TGTGAGCGAG 
GCTGTGCCTC GGACCTCCAT GTGTAGCATA CAGTCGGCAC CGCCCAAGCC ACCTACCTTG 
GAAGGCATGG TGCCAGATGA TGCTGTAGAA GCCTTGGCTG GTAGCCTGGG GAAGAAGGAA 
GCAGATCCAG GAGATGGAAA GCCTGTGGAA GATAAAGTCA AGGAGAAAGC CAAGGAAGAG 
GATCGTGAAA AACTTGGTGA AAAAGAAGAA ACAATTCCTC CTGATTATAG ATTAGAAGAG 
GTCAAGGATA AAGATGGAAA ACCACTCCTG CCAAAAGTGC CCAAGGAATC GCTTTTACCC 
ATGAGTGAAG ACTTCCTTCT TGATGCTTTG TCCAAGGACT TTGCTGGTTC CTCAAACACT 
TCACCTCTTG AATTTGAAGA GGCTAACCTT CCTGCTGTCA TCTCTGAAGT GGTTTCCCAA 
ACCCCAGCTC CAACCACGCA CTCTGCTGGT CTACCCCCTG ATACTGTGCA AAGTGACAAC 
AAAGAACTGG ACGATGCTTT GGACCAACTT TCTGACAGTC TGGGACAAAG GCAGCCTGAT 
CCAGATGAGA ACAAGCCGAT AGAGGATAAA GTCAAGGAAA GAGCTAAAGC TGAACACAGA 
GACAAGCTGG GAGAAAGAGA CGACACCATC CCCCCTGAAT ATAGACACCT CTTGGATAAC 
GATGAGGGTA AACCAGGAAA GCCACCTGCC AAGAAAACCA AGGACTCAAA GAAGCCTGAA 
GATGACAAAG ACCCCATCGA TGCCCTGTCA GGCGACTTCG ACAGCTGTCC TGCACCCACA 
GAAGCCTCAG AGAACACAGC AGAGGACAAA GCCAAGAGAA CTGCTCCCAG CTCCAAAACA 
CCGAAGAATA GGGGTAAAGC AAAGGATTCT GCAAAGGCAA AGCAGGAAAC TTCCAAGCCC 
AAAGCTGATG AAAAAAATAC AAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_005618076.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005618133.2 Canis lupus familiaris calpastatin (CAST), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe35583
Clone ID Related Accession (Same CDS sequence)	XM_005618076.2 , XM_005618076.3
Accession Version	XM_005618076.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	calpastatin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005618076.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTCG CAAGCTGGTG GTACAAGACG CATGTCAGCG AAAAAACAAG TGGATCACCT 
TCCAAGTCAG GAGAAAAGAA AGGAGCAGAT GAGAAAAAAG CAAGAAGCCT TGAGAGCAGT 
CAACCTTCCA GAACCCATGC TGGTGGAACA GCCCCAGCCA CCAAGGCGTC AGCCTCTTCT 
GCTTCTACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCTAT TCCAGTCAGC 
AAACAGACGG GAGGACAGCA TTCTCCTAAC AAGAAAAGAC ACAAAAAACA GGCTGCAAAA 
ACAGAACCTG AAAAGAAGTC ACAATCAACT AAGCCATCTG TGGTCCATGA AAAAAAAACC 
CAAGAAGTAA AGCCAAAGGA ACACAAAGAG CCAAAAAGCC TACCCAAGCA TGCATCAGAT 
ATGGGAAGCA AGCATGTTCA TAGTGAAAAA GCAGCTTCTA GATCAAGTGA GCAGCTGACA 
TCAGAGAAAT CAACAAAACC AAAGACTAAA GCACAGGATT CCGTTTCTGC CGGTGGGGAG 
AGTGTTGTTG CAGGTGCAGC TGCAGCTGCA GCATCTGCCA CAGCAGCTAA CAAGCAGAAA 
AAAGAAAAGA AATCACTAAC ATCGGCTGTA CCAGTTGAAT CCAAACCGGG TAAACCACCT 
GGAAAGTCAG ACATGGATGC TGCTTTGGAT GACTTAGTAG ACACTCTAGG AAAACCTGAA 
GAAACTAAAG AAGATAATGT ACCATATACT GGACCTGAAA TTTCAGATCC GATGACTTCC 
ACCTACATAG AGGAATTAGG TAAAAGAGAA GTCACACTTC CTCCAAAGTA CAGGGAACTT 
TTGGCAAAAA AAGAAGGTAT CACAGTGCCT CCTCCAGACT CTTCGAAACC CATGGGGCCC 
AATGATGCCA TAGATGCTTT GTCATCTGAC TTCACCTGCA GTTCACCTAC CGATGCTGGA 
AAGAAAACTA AGGAAGAGAA ATCTACAGGA GAAGAGATTT TAAAAGCCCA GCCAGCCAGG 
GTAACCAAAA GTGCTGCTTC ACCCCAAGAG AAAAAAAGAA AGGTGGAGGA GCAGGACGCA 
ATGAGTGATC AAGCACTGGA GGCTCTTTCA GCTTCTTTGG GCAGCCGGAA GCCAGAACCC 
GAGCTGGACC TCAGTTCCAT TAAGGAAGTC GACGAGGCAA AAGCCAAAGA AGAGAAACTA 
AAGAAGTGTG GTGAAGATGA GGAAACTGTC CCATCAGAGT ACAGATTAAA ACCAGCTACG 
GATAAAGATG GAAAACCTCT ATTGCCAGAG CCTGAAGAAA AATCCAAGCC CCTGAGTGAA 
TCAGAACTCA TTGATGAGCT TTCAGAAGAT TTTAACCAGC CTAAAAGTAA AGGAGAACAG 
TCTAAGCCAA CTGAAAAAAC AGAAGAATCT CAGGCTGCCC CCCTAACTCC TGTGAGCGAG 
GCTGTGCCTC GGACCTCCAT GTGTAGCATA CAGTCGGCAC CGCCCAAGCC ACCTACCTTG 
GAAGGCATGG TGCCAGATGA TGCTGTAGAA GCCTTGGCTG GTAGCCTGGG GAAGAAGGAA 
GCAGATCCAG GAGATGGAAA GCCTGTGGAA GATAAAGTCA AGGAGAAAGC CAAGGAAGAG 
GATCGTGAAA AACTTGGTGA AAAAGAAGAA ACAATTCCTC CTGATTATAG ATTAGAAGAG 
GTCAAGGATA AAGATGGAAA ACCACTCCTG CCAAAAGTGC CCAAGGAATC GCTTTTACCC 
ATGAGTGAAG ACTTCCTTCT TGATGCTTTG TCCAAGGACT TTGCTGGTTC CTCAAACACT 
TCACCTCTTG AATTTGAAGA GGCTAACCTT CCTGCTGTCA TCTCTGAAGT GGTTTCCCAA 
ACCCCAGCTC CAACCACGCA CTCTGCTGGT CTACCCCCTG ATACTGTGCA AAGTGACAAC 
AAAGAACTGG ACGATGCTTT GGACCAACTT TCTGACAGTC TGGGACAAAG GCAGCCTGAT 
CCAGATGAGA ACAAGCCGAT AGAGGATAAA GTCAAGGAAA GAGCTAAAGC TGAACACAGA 
GACAAGCTGG GAGAAAGAGA CGACACCATC CCCCCTGAAT ATAGACACCT CTTGGATAAC 
GATGAGGGTA AACCAGGAAA GCCACCTGCC AAGAAAACCA AGGACTCAAA GAAGCCTGAA 
GATGACAAAG ACCCCATCGA TGCCCTGTCA GGCGACTTCG ACAGCTGTCC TGCACCCACA 
GAAGCCTCAG AGAACACAGC AGAGGACAAA GCCAAGAGAA CTGCTCCCAG CTCCAAAACA 
CCGAAGAATA GGGGTAAAGC AAAGGATTCT GCAAAGGCAA AGCAGGAAAC TTCCAAGCCC 
AAAGCTGATG AAAAAAATAC AAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005618133.2
CDS	120..2486
Translation	MAFASWWYKTHVSEKTSGSPSKSGEKKGADEKKARSLESSQPSRTHAGGTAPATKASASSASTSKSSSMNPTETKAIPVSKQTGGQHSPNKKRHKKQAAKTEPEKKSQSTKPSVVHEKKTQEVKPKEHKEPKSLPKHASDMGSKHVHSEKAASRSSEQLTSEKSTKPKTKAQDSVSAGGESVVAGAAAAAASATAANKQKKEKKSLTSAVPVESKPGKPPGKSDMDAALDDLVDTLGKPEETKEDNVPYTGPEISDPMTSTYIEELGKREVTLPPKYRELLAKKEGITVPPPDSSKPMGPNDAIDALSSDFTCSSPTDAGKKTKEEKSTGEEILKAQPARVTKSAASPQEKKRKVEEQDAMSDQALEALSASLGSRKPEPELDLSSIKEVDEAKAKEEKLKKCGEDEETVPSEYRLKPATDKDGKPLLPEPEEKSKPLSESELIDELSEDFNQPKSKGEQSKPTEKTEESQAAPLTPVSEAVPRTSMCSIQSAPPKPPTLEGMVPDDAVEALAGSLGKKEADPGDGKPVEDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKVPKESLLPMSEDFLLDALSKDFAGSSNTSPLEFEEANLPAVISEVVSQTPAPTTHSAGLPPDTVQSDNKELDDALDQLSDSLGQRQPDPDENKPIEDKVKERAKAEHRDKLGERDDTIPPEYRHLLDNDEGKPGKPPAKKTKDSKKPEDDKDPIDALSGDFDSCPAPTEASENTAEDKAKRTAPSSKTPKNRGKAKDSAKAKQETSKPKADEKNTS

Target ORF information:

RefSeq Version	XM_005618076.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calpastatin (CAST), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005618076.3

ORF Insert Sequence:

ATGGCATTCG CAAGCTGGTG GTACAAGACG CATGTCAGCG AAAAAACAAG TGGATCACCT 
TCCAAGTCAG GAGAAAAGAA AGGAGCAGAT GAGAAAAAAG CAAGAAGCCT TGAGAGCAGT 
CAACCTTCCA GAACCCATGC TGGTGGAACA GCCCCAGCCA CCAAGGCGTC AGCCTCTTCT 
GCTTCTACCA GCAAGTCTTC CAGTATGAAT CCCACAGAAA CCAAGGCTAT TCCAGTCAGC 
AAACAGACGG GAGGACAGCA TTCTCCTAAC AAGAAAAGAC ACAAAAAACA GGCTGCAAAA 
ACAGAACCTG AAAAGAAGTC ACAATCAACT AAGCCATCTG TGGTCCATGA AAAAAAAACC 
CAAGAAGTAA AGCCAAAGGA ACACAAAGAG CCAAAAAGCC TACCCAAGCA TGCATCAGAT 
ATGGGAAGCA AGCATGTTCA TAGTGAAAAA GCAGCTTCTA GATCAAGTGA GCAGCTGACA 
TCAGAGAAAT CAACAAAACC AAAGACTAAA GCACAGGATT CCGTTTCTGC CGGTGGGGAG 
AGTGTTGTTG CAGGTGCAGC TGCAGCTGCA GCATCTGCCA CAGCAGCTAA CAAGCAGAAA 
AAAGAAAAGA AATCACTAAC ATCGGCTGTA CCAGTTGAAT CCAAACCGGG TAAACCACCT 
GGAAAGTCAG ACATGGATGC TGCTTTGGAT GACTTAGTAG ACACTCTAGG AAAACCTGAA 
GAAACTAAAG AAGATAATGT ACCATATACT GGACCTGAAA TTTCAGATCC GATGACTTCC 
ACCTACATAG AGGAATTAGG TAAAAGAGAA GTCACACTTC CTCCAAAGTA CAGGGAACTT 
TTGGCAAAAA AAGAAGGTAT CACAGTGCCT CCTCCAGACT CTTCGAAACC CATGGGGCCC 
AATGATGCCA TAGATGCTTT GTCATCTGAC TTCACCTGCA GTTCACCTAC CGATGCTGGA 
AAGAAAACTA AGGAAGAGAA ATCTACAGGA GAAGAGATTT TAAAAGCCCA GCCAGCCAGG 
GTAACCAAAA GTGCTGCTTC ACCCCAAGAG AAAAAAAGAA AGGTGGAGGA GCAGGACGCA 
ATGAGTGATC AAGCACTGGA GGCTCTTTCA GCTTCTTTGG GCAGCCGGAA GCCAGAACCC 
GAGCTGGACC TCAGTTCCAT TAAGGAAGTC GACGAGGCAA AAGCCAAAGA AGAGAAACTA 
AAGAAGTGTG GTGAAGATGA GGAAACTGTC CCATCAGAGT ACAGATTAAA ACCAGCTACG 
GATAAAGATG GAAAACCTCT ATTGCCAGAG CCTGAAGAAA AATCCAAGCC CCTGAGTGAA 
TCAGAACTCA TTGATGAGCT TTCAGAAGAT TTTAACCAGC CTAAAAGTAA AGGAGAACAG 
TCTAAGCCAA CTGAAAAAAC AGAAGAATCT CAGGCTGCCC CCCTAACTCC TGTGAGCGAG 
GCTGTGCCTC GGACCTCCAT GTGTAGCATA CAGTCGGCAC CGCCCAAGCC ACCTACCTTG 
GAAGGCATGG TGCCAGATGA TGCTGTAGAA GCCTTGGCTG GTAGCCTGGG GAAGAAGGAA 
GCAGATCCAG GAGATGGAAA GCCTGTGGAA GATAAAGTCA AGGAGAAAGC CAAGGAAGAG 
GATCGTGAAA AACTTGGTGA AAAAGAAGAA ACAATTCCTC CTGATTATAG ATTAGAAGAG 
GTCAAGGATA AAGATGGAAA ACCACTCCTG CCAAAAGTGC CCAAGGAATC GCTTTTACCC 
ATGAGTGAAG ACTTCCTTCT TGATGCTTTG TCCAAGGACT TTGCTGGTTC CTCAAACACT 
TCACCTCTTG AATTTGAAGA GGCTAACCTT CCTGCTGTCA TCTCTGAAGT GGTTTCCCAA 
ACCCCAGCTC CAACCACGCA CTCTGCTGGT CTACCCCCTG ATACTGTGCA AAGTGACAAC 
AAAGAACTGG ACGATGCTTT GGACCAACTT TCTGACAGTC TGGGACAAAG GCAGCCTGAT 
CCAGATGAGA ACAAGCCGAT AGAGGATAAA GTCAAGGAAA GAGCTAAAGC TGAACACAGA 
GACAAGCTGG GAGAAAGAGA CGACACCATC CCCCCTGAAT ATAGACACCT CTTGGATAAC 
GATGAGGGTA AACCAGGAAA GCCACCTGCC AAGAAAACCA AGGACTCAAA GAAGCCTGAA 
GATGACAAAG ACCCCATCGA TGCCCTGTCA GGCGACTTCG ACAGCTGTCC TGCACCCACA 
GAAGCCTCAG AGAACACAGC AGAGGACAAA GCCAAGAGAA CTGCTCCCAG CTCCAAAACA 
CCGAAGAATA GGGGTAAAGC AAAGGATTCT GCAAAGGCAA AGCAGGAAAC TTCCAAGCCC 
AAAGCTGATG AAAAAAATAC AAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_005618081.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005618138.2 Canis lupus familiaris calpastatin (CAST), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OCe35592
Clone ID Related Accession (Same CDS sequence)	XM_005618081.3 , XM_005618081.2
Accession Version	XM_005618081.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	calpastatin isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005618081.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCCA CAGAAACCAA GGCTGCAAAA ACAGAACCTG AAAAGAAGTC ACAATCAACT 
AAGCCATCTG TGGTCCATGA AAAAAAAACC CAAGAAGTAA AGCCAAAGGA ACACAAAGAG 
CCAAAAAGCC TACCCAAGCA TGCATCAGAT ATGGGAAGCA AGCATGTTCA TAGTGAAAAA 
GCAGCTTCTA GATCAAGTGA GCAGCTGACA TCAGAGAAAT CAACAAAACC AAAGACTAAA 
GCACAGGATT CCGTTTCTGC CGGTGGGGAG AGTGTTGTTG CAGGTGCAGC TGCAGCTGCA 
GCATCTGCCA CAGCAGCTAA CAAGCAGAAA AAAGAAAAGA AATCACTAAC ATCGGCTGTA 
CCAGTTGAAT CCAAACCGGG TAAACCACCT GGAAAGTCAG ACATGGATGC TGCTTTGGAT 
GACTTAGTAG ACACTCTAGG AAAACCTGAA GAAACTAAAG AAGATAATGT ACCATATACT 
GGACCTGAAA TTTCAGATCC GATGACTTCC ACCTACATAG AGGAATTAGG TAAAAGAGAA 
GTCACACTTC CTCCAAAGTA CAGGGAACTT TTGGCAAAAA AAGAAGGTAT CACAGTGCCT 
CCTCCAGACT CTTCGAAACC CATGGGGCCC AATGATGCCA TAGATGCTTT GTCATCTGAC 
TTCACCTGCA GTTCACCTAC CGATGCTGGA AAGAAAACTA AGGAAGAGAA ATCTACAGGA 
GAAGAGATTT TAAAAGCCCA GCCAGCCAGG GTAACCAAAA GTGCTGCTTC ACCCCAAGAG 
AAAAAAAGAA AGGTGGAGGA GCAGGACGCA ATGAGTGATC AAGCACTGGA GGCTCTTTCA 
GCTTCTTTGG GCAGCCGGAA GCCAGAACCC GAGCTGGACC TCAGTTCCAT TAAGGAAGTC 
GACGAGGCAA AAGCCAAAGA AGAGAAACTA AAGAAGTGTG GTGAAGATGA GGAAACTGTC 
CCATCAGAGT ACAGATTAAA ACCAGCTACG GATAAAGATG GAAAACCTCT ATTGCCAGAG 
CCTGAAGAAA AATCCAAGCC CCTGAGTGAA TCAGAACTCA TTGATGAGCT TTCAGAAGAT 
TTTAACCAGC CTAAAAGTAA AGGAGAACAG TCTAAGCCAA CTGAAAAAAC AGAAGAATCT 
CAGGCTGCCC CCCTAACTCC TGTGAGCGAG GCTGTGCCTC GGACCTCCAT GTGTAGCATA 
CAGTCGGCAC CGCCCAAGCC ACCTACCTTG GAAGGCATGG TGCCAGATGA TGCTGTAGAA 
GCCTTGGCTG GTAGCCTGGG GAAGAAGGAA GCAGATCCAG GAGATGGAAA GCCTGTGGAA 
GATAAAGTCA AGGAGAAAGC CAAGGAAGAG GATCGTGAAA AACTTGGTGA AAAAGAAGAA 
ACAATTCCTC CTGATTATAG ATTAGAAGAG GTCAAGGATA AAGATGGAAA ACCACTCCTG 
CCAAAAGTGC CCAAGGAATC GCTTTTACCC ATGAGTGAAG ACTTCCTTCT TGATGCTTTG 
TCCAAGGACT TTGCTGGTTC CTCAAACACT TCACCTCTTG AATTTGAAGA GGCTAACCTT 
CCTGCTGTCA TCTCTGAAGT GGTTTCCCAA ACCCCAGCTC CAACCACGCA CTCTGCTGGT 
CTACCCCCTG ATACTGTGCA AAGTGACAAC AAAGAACTGG ACGATGCTTT GGACCAACTT 
TCTGACAGTC TGGGACAAAG GCAGCCTGAT CCAGATGAGA ACAAGCCGAT AGAGGATAAA 
GTCAAGGAAA GAGCTAAAGC TGAACACAGA GACAAGCTGG GAGAAAGAGA CGACACCATC 
CCCCCTGAAT ATAGACACCT CTTGGATAAC GATGAGGGTA AACCAGGAAA GCCACCTGCC 
AAGAAAACCA AGGACTCAAA GAAGCCTGAA GATGACAAAG ACCCCATCGA TGCCCTGTCA 
GGCGACTTCG ACAGCTGTCC TGCACCCACA GAAGCCTCAG AGAACACAGC AGAGGACAAA 
GCCAAGAGAA CTGCTCCCAG CTCCAAAACA CCGAAGAATA GGGGTAAAGC AAAGGATTCT 
GCAAAGGCAA AGCAGGAAAC TTCCAAGCCC AAAGCTGATG AAAAAAATAC AAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005618138.2
CDS	173..2269
Translation	MNPTETKAAKTEPEKKSQSTKPSVVHEKKTQEVKPKEHKEPKSLPKHASDMGSKHVHSEKAASRSSEQLTSEKSTKPKTKAQDSVSAGGESVVAGAAAAAASATAANKQKKEKKSLTSAVPVESKPGKPPGKSDMDAALDDLVDTLGKPEETKEDNVPYTGPEISDPMTSTYIEELGKREVTLPPKYRELLAKKEGITVPPPDSSKPMGPNDAIDALSSDFTCSSPTDAGKKTKEEKSTGEEILKAQPARVTKSAASPQEKKRKVEEQDAMSDQALEALSASLGSRKPEPELDLSSIKEVDEAKAKEEKLKKCGEDEETVPSEYRLKPATDKDGKPLLPEPEEKSKPLSESELIDELSEDFNQPKSKGEQSKPTEKTEESQAAPLTPVSEAVPRTSMCSIQSAPPKPPTLEGMVPDDAVEALAGSLGKKEADPGDGKPVEDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKVPKESLLPMSEDFLLDALSKDFAGSSNTSPLEFEEANLPAVISEVVSQTPAPTTHSAGLPPDTVQSDNKELDDALDQLSDSLGQRQPDPDENKPIEDKVKERAKAEHRDKLGERDDTIPPEYRHLLDNDEGKPGKPPAKKTKDSKKPEDDKDPIDALSGDFDSCPAPTEASENTAEDKAKRTAPSSKTPKNRGKAKDSAKAKQETSKPKADEKNTS

Target ORF information:

RefSeq Version	XM_005618081.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calpastatin (CAST), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005618081.3

ORF Insert Sequence:

ATGAATCCCA CAGAAACCAA GGCTGCAAAA ACAGAACCTG AAAAGAAGTC ACAATCAACT 
AAGCCATCTG TGGTCCATGA AAAAAAAACC CAAGAAGTAA AGCCAAAGGA ACACAAAGAG 
CCAAAAAGCC TACCCAAGCA TGCATCAGAT ATGGGAAGCA AGCATGTTCA TAGTGAAAAA 
GCAGCTTCTA GATCAAGTGA GCAGCTGACA TCAGAGAAAT CAACAAAACC AAAGACTAAA 
GCACAGGATT CCGTTTCTGC CGGTGGGGAG AGTGTTGTTG CAGGTGCAGC TGCAGCTGCA 
GCATCTGCCA CAGCAGCTAA CAAGCAGAAA AAAGAAAAGA AATCACTAAC ATCGGCTGTA 
CCAGTTGAAT CCAAACCGGG TAAACCACCT GGAAAGTCAG ACATGGATGC TGCTTTGGAT 
GACTTAGTAG ACACTCTAGG AAAACCTGAA GAAACTAAAG AAGATAATGT ACCATATACT 
GGACCTGAAA TTTCAGATCC GATGACTTCC ACCTACATAG AGGAATTAGG TAAAAGAGAA 
GTCACACTTC CTCCAAAGTA CAGGGAACTT TTGGCAAAAA AAGAAGGTAT CACAGTGCCT 
CCTCCAGACT CTTCGAAACC CATGGGGCCC AATGATGCCA TAGATGCTTT GTCATCTGAC 
TTCACCTGCA GTTCACCTAC CGATGCTGGA AAGAAAACTA AGGAAGAGAA ATCTACAGGA 
GAAGAGATTT TAAAAGCCCA GCCAGCCAGG GTAACCAAAA GTGCTGCTTC ACCCCAAGAG 
AAAAAAAGAA AGGTGGAGGA GCAGGACGCA ATGAGTGATC AAGCACTGGA GGCTCTTTCA 
GCTTCTTTGG GCAGCCGGAA GCCAGAACCC GAGCTGGACC TCAGTTCCAT TAAGGAAGTC 
GACGAGGCAA AAGCCAAAGA AGAGAAACTA AAGAAGTGTG GTGAAGATGA GGAAACTGTC 
CCATCAGAGT ACAGATTAAA ACCAGCTACG GATAAAGATG GAAAACCTCT ATTGCCAGAG 
CCTGAAGAAA AATCCAAGCC CCTGAGTGAA TCAGAACTCA TTGATGAGCT TTCAGAAGAT 
TTTAACCAGC CTAAAAGTAA AGGAGAACAG TCTAAGCCAA CTGAAAAAAC AGAAGAATCT 
CAGGCTGCCC CCCTAACTCC TGTGAGCGAG GCTGTGCCTC GGACCTCCAT GTGTAGCATA 
CAGTCGGCAC CGCCCAAGCC ACCTACCTTG GAAGGCATGG TGCCAGATGA TGCTGTAGAA 
GCCTTGGCTG GTAGCCTGGG GAAGAAGGAA GCAGATCCAG GAGATGGAAA GCCTGTGGAA 
GATAAAGTCA AGGAGAAAGC CAAGGAAGAG GATCGTGAAA AACTTGGTGA AAAAGAAGAA 
ACAATTCCTC CTGATTATAG ATTAGAAGAG GTCAAGGATA AAGATGGAAA ACCACTCCTG 
CCAAAAGTGC CCAAGGAATC GCTTTTACCC ATGAGTGAAG ACTTCCTTCT TGATGCTTTG 
TCCAAGGACT TTGCTGGTTC CTCAAACACT TCACCTCTTG AATTTGAAGA GGCTAACCTT 
CCTGCTGTCA TCTCTGAAGT GGTTTCCCAA ACCCCAGCTC CAACCACGCA CTCTGCTGGT 
CTACCCCCTG ATACTGTGCA AAGTGACAAC AAAGAACTGG ACGATGCTTT GGACCAACTT 
TCTGACAGTC TGGGACAAAG GCAGCCTGAT CCAGATGAGA ACAAGCCGAT AGAGGATAAA 
GTCAAGGAAA GAGCTAAAGC TGAACACAGA GACAAGCTGG GAGAAAGAGA CGACACCATC 
CCCCCTGAAT ATAGACACCT CTTGGATAAC GATGAGGGTA AACCAGGAAA GCCACCTGCC 
AAGAAAACCA AGGACTCAAA GAAGCCTGAA GATGACAAAG ACCCCATCGA TGCCCTGTCA 
GGCGACTTCG ACAGCTGTCC TGCACCCACA GAAGCCTCAG AGAACACAGC AGAGGACAAA 
GCCAAGAGAA CTGCTCCCAG CTCCAAAACA CCGAAGAATA GGGGTAAAGC AAAGGATTCT 
GCAAAGGCAA AGCAGGAAAC TTCCAAGCCC AAAGCTGATG AAAAAAATAC AAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_005618081.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005618138.2 Canis lupus familiaris calpastatin (CAST), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OCe35592
Clone ID Related Accession (Same CDS sequence)	XM_005618081.3 , XM_005618081.2
Accession Version	XM_005618081.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	calpastatin isoform X14
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726052.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005618081.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCCA CAGAAACCAA GGCTGCAAAA ACAGAACCTG AAAAGAAGTC ACAATCAACT 
AAGCCATCTG TGGTCCATGA AAAAAAAACC CAAGAAGTAA AGCCAAAGGA ACACAAAGAG 
CCAAAAAGCC TACCCAAGCA TGCATCAGAT ATGGGAAGCA AGCATGTTCA TAGTGAAAAA 
GCAGCTTCTA GATCAAGTGA GCAGCTGACA TCAGAGAAAT CAACAAAACC AAAGACTAAA 
GCACAGGATT CCGTTTCTGC CGGTGGGGAG AGTGTTGTTG CAGGTGCAGC TGCAGCTGCA 
GCATCTGCCA CAGCAGCTAA CAAGCAGAAA AAAGAAAAGA AATCACTAAC ATCGGCTGTA 
CCAGTTGAAT CCAAACCGGG TAAACCACCT GGAAAGTCAG ACATGGATGC TGCTTTGGAT 
GACTTAGTAG ACACTCTAGG AAAACCTGAA GAAACTAAAG AAGATAATGT ACCATATACT 
GGACCTGAAA TTTCAGATCC GATGACTTCC ACCTACATAG AGGAATTAGG TAAAAGAGAA 
GTCACACTTC CTCCAAAGTA CAGGGAACTT TTGGCAAAAA AAGAAGGTAT CACAGTGCCT 
CCTCCAGACT CTTCGAAACC CATGGGGCCC AATGATGCCA TAGATGCTTT GTCATCTGAC 
TTCACCTGCA GTTCACCTAC CGATGCTGGA AAGAAAACTA AGGAAGAGAA ATCTACAGGA 
GAAGAGATTT TAAAAGCCCA GCCAGCCAGG GTAACCAAAA GTGCTGCTTC ACCCCAAGAG 
AAAAAAAGAA AGGTGGAGGA GCAGGACGCA ATGAGTGATC AAGCACTGGA GGCTCTTTCA 
GCTTCTTTGG GCAGCCGGAA GCCAGAACCC GAGCTGGACC TCAGTTCCAT TAAGGAAGTC 
GACGAGGCAA AAGCCAAAGA AGAGAAACTA AAGAAGTGTG GTGAAGATGA GGAAACTGTC 
CCATCAGAGT ACAGATTAAA ACCAGCTACG GATAAAGATG GAAAACCTCT ATTGCCAGAG 
CCTGAAGAAA AATCCAAGCC CCTGAGTGAA TCAGAACTCA TTGATGAGCT TTCAGAAGAT 
TTTAACCAGC CTAAAAGTAA AGGAGAACAG TCTAAGCCAA CTGAAAAAAC AGAAGAATCT 
CAGGCTGCCC CCCTAACTCC TGTGAGCGAG GCTGTGCCTC GGACCTCCAT GTGTAGCATA 
CAGTCGGCAC CGCCCAAGCC ACCTACCTTG GAAGGCATGG TGCCAGATGA TGCTGTAGAA 
GCCTTGGCTG GTAGCCTGGG GAAGAAGGAA GCAGATCCAG GAGATGGAAA GCCTGTGGAA 
GATAAAGTCA AGGAGAAAGC CAAGGAAGAG GATCGTGAAA AACTTGGTGA AAAAGAAGAA 
ACAATTCCTC CTGATTATAG ATTAGAAGAG GTCAAGGATA AAGATGGAAA ACCACTCCTG 
CCAAAAGTGC CCAAGGAATC GCTTTTACCC ATGAGTGAAG ACTTCCTTCT TGATGCTTTG 
TCCAAGGACT TTGCTGGTTC CTCAAACACT TCACCTCTTG AATTTGAAGA GGCTAACCTT 
CCTGCTGTCA TCTCTGAAGT GGTTTCCCAA ACCCCAGCTC CAACCACGCA CTCTGCTGGT 
CTACCCCCTG ATACTGTGCA AAGTGACAAC AAAGAACTGG ACGATGCTTT GGACCAACTT 
TCTGACAGTC TGGGACAAAG GCAGCCTGAT CCAGATGAGA ACAAGCCGAT AGAGGATAAA 
GTCAAGGAAA GAGCTAAAGC TGAACACAGA GACAAGCTGG GAGAAAGAGA CGACACCATC 
CCCCCTGAAT ATAGACACCT CTTGGATAAC GATGAGGGTA AACCAGGAAA GCCACCTGCC 
AAGAAAACCA AGGACTCAAA GAAGCCTGAA GATGACAAAG ACCCCATCGA TGCCCTGTCA 
GGCGACTTCG ACAGCTGTCC TGCACCCACA GAAGCCTCAG AGAACACAGC AGAGGACAAA 
GCCAAGAGAA CTGCTCCCAG CTCCAAAACA CCGAAGAATA GGGGTAAAGC AAAGGATTCT 
GCAAAGGCAA AGCAGGAAAC TTCCAAGCCC AAAGCTGATG AAAAAAATAC AAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005618138.2
CDS	122..2218
Translation	MNPTETKAAKTEPEKKSQSTKPSVVHEKKTQEVKPKEHKEPKSLPKHASDMGSKHVHSEKAASRSSEQLTSEKSTKPKTKAQDSVSAGGESVVAGAAAAAASATAANKQKKEKKSLTSAVPVESKPGKPPGKSDMDAALDDLVDTLGKPEETKEDNVPYTGPEISDPMTSTYIEELGKREVTLPPKYRELLAKKEGITVPPPDSSKPMGPNDAIDALSSDFTCSSPTDAGKKTKEEKSTGEEILKAQPARVTKSAASPQEKKRKVEEQDAMSDQALEALSASLGSRKPEPELDLSSIKEVDEAKAKEEKLKKCGEDEETVPSEYRLKPATDKDGKPLLPEPEEKSKPLSESELIDELSEDFNQPKSKGEQSKPTEKTEESQAAPLTPVSEAVPRTSMCSIQSAPPKPPTLEGMVPDDAVEALAGSLGKKEADPGDGKPVEDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKVPKESLLPMSEDFLLDALSKDFAGSSNTSPLEFEEANLPAVISEVVSQTPAPTTHSAGLPPDTVQSDNKELDDALDQLSDSLGQRQPDPDENKPIEDKVKERAKAEHRDKLGERDDTIPPEYRHLLDNDEGKPGKPPAKKTKDSKKPEDDKDPIDALSGDFDSCPAPTEASENTAEDKAKRTAPSSKTPKNRGKAKDSAKAKQETSKPKADEKNTS

Target ORF information:

RefSeq Version	XM_005618081.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calpastatin (CAST), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005618081.2

ORF Insert Sequence:

ATGAATCCCA CAGAAACCAA GGCTGCAAAA ACAGAACCTG AAAAGAAGTC ACAATCAACT 
AAGCCATCTG TGGTCCATGA AAAAAAAACC CAAGAAGTAA AGCCAAAGGA ACACAAAGAG 
CCAAAAAGCC TACCCAAGCA TGCATCAGAT ATGGGAAGCA AGCATGTTCA TAGTGAAAAA 
GCAGCTTCTA GATCAAGTGA GCAGCTGACA TCAGAGAAAT CAACAAAACC AAAGACTAAA 
GCACAGGATT CCGTTTCTGC CGGTGGGGAG AGTGTTGTTG CAGGTGCAGC TGCAGCTGCA 
GCATCTGCCA CAGCAGCTAA CAAGCAGAAA AAAGAAAAGA AATCACTAAC ATCGGCTGTA 
CCAGTTGAAT CCAAACCGGG TAAACCACCT GGAAAGTCAG ACATGGATGC TGCTTTGGAT 
GACTTAGTAG ACACTCTAGG AAAACCTGAA GAAACTAAAG AAGATAATGT ACCATATACT 
GGACCTGAAA TTTCAGATCC GATGACTTCC ACCTACATAG AGGAATTAGG TAAAAGAGAA 
GTCACACTTC CTCCAAAGTA CAGGGAACTT TTGGCAAAAA AAGAAGGTAT CACAGTGCCT 
CCTCCAGACT CTTCGAAACC CATGGGGCCC AATGATGCCA TAGATGCTTT GTCATCTGAC 
TTCACCTGCA GTTCACCTAC CGATGCTGGA AAGAAAACTA AGGAAGAGAA ATCTACAGGA 
GAAGAGATTT TAAAAGCCCA GCCAGCCAGG GTAACCAAAA GTGCTGCTTC ACCCCAAGAG 
AAAAAAAGAA AGGTGGAGGA GCAGGACGCA ATGAGTGATC AAGCACTGGA GGCTCTTTCA 
GCTTCTTTGG GCAGCCGGAA GCCAGAACCC GAGCTGGACC TCAGTTCCAT TAAGGAAGTC 
GACGAGGCAA AAGCCAAAGA AGAGAAACTA AAGAAGTGTG GTGAAGATGA GGAAACTGTC 
CCATCAGAGT ACAGATTAAA ACCAGCTACG GATAAAGATG GAAAACCTCT ATTGCCAGAG 
CCTGAAGAAA AATCCAAGCC CCTGAGTGAA TCAGAACTCA TTGATGAGCT TTCAGAAGAT 
TTTAACCAGC CTAAAAGTAA AGGAGAACAG TCTAAGCCAA CTGAAAAAAC AGAAGAATCT 
CAGGCTGCCC CCCTAACTCC TGTGAGCGAG GCTGTGCCTC GGACCTCCAT GTGTAGCATA 
CAGTCGGCAC CGCCCAAGCC ACCTACCTTG GAAGGCATGG TGCCAGATGA TGCTGTAGAA 
GCCTTGGCTG GTAGCCTGGG GAAGAAGGAA GCAGATCCAG GAGATGGAAA GCCTGTGGAA 
GATAAAGTCA AGGAGAAAGC CAAGGAAGAG GATCGTGAAA AACTTGGTGA AAAAGAAGAA 
ACAATTCCTC CTGATTATAG ATTAGAAGAG GTCAAGGATA AAGATGGAAA ACCACTCCTG 
CCAAAAGTGC CCAAGGAATC GCTTTTACCC ATGAGTGAAG ACTTCCTTCT TGATGCTTTG 
TCCAAGGACT TTGCTGGTTC CTCAAACACT TCACCTCTTG AATTTGAAGA GGCTAACCTT 
CCTGCTGTCA TCTCTGAAGT GGTTTCCCAA ACCCCAGCTC CAACCACGCA CTCTGCTGGT 
CTACCCCCTG ATACTGTGCA AAGTGACAAC AAAGAACTGG ACGATGCTTT GGACCAACTT 
TCTGACAGTC TGGGACAAAG GCAGCCTGAT CCAGATGAGA ACAAGCCGAT AGAGGATAAA 
GTCAAGGAAA GAGCTAAAGC TGAACACAGA GACAAGCTGG GAGAAAGAGA CGACACCATC 
CCCCCTGAAT ATAGACACCT CTTGGATAAC GATGAGGGTA AACCAGGAAA GCCACCTGCC 
AAGAAAACCA AGGACTCAAA GAAGCCTGAA GATGACAAAG ACCCCATCGA TGCCCTGTCA 
GGCGACTTCG ACAGCTGTCC TGCACCCACA GAAGCCTCAG AGAACACAGC AGAGGACAAA 
GCCAAGAGAA CTGCTCCCAG CTCCAAAACA CCGAAGAATA GGGGTAAAGC AAAGGATTCT 
GCAAAGGCAA AGCAGGAAAC TTCCAAGCCC AAAGCTGATG AAAAAAATAC AAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_005618082.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005618139.2 Canis lupus familiaris calpastatin (CAST), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OCe35593
Clone ID Related Accession (Same CDS sequence)	XM_005618082.3 , XM_005618082.2
Accession Version	XM_005618082.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	calpastatin isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005618082.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCCA CAGAAACCAA GGCTATTCCA GTCAGCAAAC AGACGGGAGG ACAGCATTCT 
CCTAACAAGA AAAGACACAA AAAACAGGCT GCAAAAACAG AACCTGAAAA GAAGTCACAA 
TCAACTAAGC CATCTGTGGT CCATGAAAAA AAAACCCAAG AAGTAAAGCC AAAGGAACAC 
AAAGAGCCAA AAAGCCTACC CAAGCATGCA TCAGATATGG GAAGCAAGCA TGTTCATAGT 
GAAAAAGCAG CTTCTAGATC AAGTGAGCAG CTGACATCAG AGAAATCAAC AAAACCAAAG 
ACTAAAGCAC AGGATTCCGT TTCTGCCGGT GGGGAGAGTG TTGTTGCAGG TGCAGCTGCA 
GCTGCAGCAT CTGCCACAGC AGCTAACAAG TCAGACATGG ATGCTGCTTT GGATGACTTA 
GTAGACACTC TAGGAAAACC TGAAGAAACT AAAGAAGATA ATGTACCATA TACTGGACCT 
GAAATTTCAG ATCCGATGAC TTCCACCTAC ATAGAGGAAT TAGGTAAAAG AGAAGTCACA 
CTTCCTCCAA AGTACAGGGA ACTTTTGGCA AAAAAAGAAG GTATCACAGT GCCTCCTCCA 
GACTCTTCGA AACCCATGGG GCCCAATGAT GCCATAGATG CTTTGTCATC TGACTTCACC 
TGCAGTTCAC CTACCGATGC TGGAAAGAAA ACTAAGGAAG AGAAATCTAC AGGAGAAGAG 
ATTTTAAAAG CCCAGCCAGC CAGGGTAACC AAAAGTGCTG CTTCACCCCA AGAGAAAAAA 
AGAAAGGTGG AGGAGCAGGA CGCAATGAGT GATCAAGCAC TGGAGGCTCT TTCAGCTTCT 
TTGGGCAGCC GGAAGCCAGA ACCCGAGCTG GACCTCAGTT CCATTAAGGA AGTCGACGAG 
GCAAAAGCCA AAGAAGAGAA ACTAAAGAAG TGTGGTGAAG ATGAGGAAAC TGTCCCATCA 
GAGTACAGAT TAAAACCAGC TACGGATAAA GATGGAAAAC CTCTATTGCC AGAGCCTGAA 
GAAAAATCCA AGCCCCTGAG TGAATCAGAA CTCATTGATG AGCTTTCAGA AGATTTTAAC 
CAGCCTAAAA GTAAAGGAGA ACAGTCTAAG CCAACTGAAA AAACAGAAGA ATCTCAGGCT 
GCCCCCCTAA CTCCTGTGAG CGAGGCTGTG CCTCGGACCT CCATGTGTAG CATACAGTCG 
GCACCGCCCA AGCCACCTAC CTTGGAAGGC ATGGTGCCAG ATGATGCTGT AGAAGCCTTG 
GCTGGTAGCC TGGGGAAGAA GGAAGCAGAT CCAGGAGATG GAAAGCCTGT GGAAGATAAA 
GTCAAGGAGA AAGCCAAGGA AGAGGATCGT GAAAAACTTG GTGAAAAAGA AGAAACAATT 
CCTCCTGATT ATAGATTAGA AGAGGTCAAG GATAAAGATG GAAAACCACT CCTGCCAAAA 
GTGCCCAAGG AATCGCTTTT ACCCATGAGT GAAGACTTCC TTCTTGATGC TTTGTCCAAG 
GACTTTGCTG GTTCCTCAAA CACTTCACCT CTTGAATTTG AAGAGGCTAA CCTTCCTGCT 
GTCATCTCTG AAGTGGTTTC CCAAACCCCA GCTCCAACCA CGCACTCTGC TGGTCTACCC 
CCTGATACTG TGCAAAGTGA CAACAAAGAA CTGGACGATG CTTTGGACCA ACTTTCTGAC 
AGTCTGGGAC AAAGGCAGCC TGATCCAGAT GAGAACAAGC CGATAGAGGA TAAAGTCAAG 
GAAAGAGCTA AAGCTGAACA CAGAGACAAG CTGGGAGAAA GAGACGACAC CATCCCCCCT 
GAATATAGAC ACCTCTTGGA TAACGATGAG GGTAAACCAG GAAAGCCACC TGCCAAGAAA 
ACCAAGGACT CAAAGAAGCC TGAAGATGAC AAAGACCCCA TCGATGCCCT GTCAGGCGAC 
TTCGACAGCT GTCCTGCACC CACAGAAGCC TCAGAGAACA CAGCAGAGGA CAAAGCCAAG 
AGAACTGCTC CCAGCTCCAA AACACCGAAG AATAGGGGTA AAGCAAAGGA TTCTGCAAAG 
GCAAAGCAGG AAACTTCCAA GCCCAAAGCT GATGAAAAAA ATACAAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005618139.2
CDS	173..2263
Translation	MNPTETKAIPVSKQTGGQHSPNKKRHKKQAAKTEPEKKSQSTKPSVVHEKKTQEVKPKEHKEPKSLPKHASDMGSKHVHSEKAASRSSEQLTSEKSTKPKTKAQDSVSAGGESVVAGAAAAAASATAANKSDMDAALDDLVDTLGKPEETKEDNVPYTGPEISDPMTSTYIEELGKREVTLPPKYRELLAKKEGITVPPPDSSKPMGPNDAIDALSSDFTCSSPTDAGKKTKEEKSTGEEILKAQPARVTKSAASPQEKKRKVEEQDAMSDQALEALSASLGSRKPEPELDLSSIKEVDEAKAKEEKLKKCGEDEETVPSEYRLKPATDKDGKPLLPEPEEKSKPLSESELIDELSEDFNQPKSKGEQSKPTEKTEESQAAPLTPVSEAVPRTSMCSIQSAPPKPPTLEGMVPDDAVEALAGSLGKKEADPGDGKPVEDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKVPKESLLPMSEDFLLDALSKDFAGSSNTSPLEFEEANLPAVISEVVSQTPAPTTHSAGLPPDTVQSDNKELDDALDQLSDSLGQRQPDPDENKPIEDKVKERAKAEHRDKLGERDDTIPPEYRHLLDNDEGKPGKPPAKKTKDSKKPEDDKDPIDALSGDFDSCPAPTEASENTAEDKAKRTAPSSKTPKNRGKAKDSAKAKQETSKPKADEKNTS

Target ORF information:

RefSeq Version	XM_005618082.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calpastatin (CAST), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005618082.3

ORF Insert Sequence:

ATGAATCCCA CAGAAACCAA GGCTATTCCA GTCAGCAAAC AGACGGGAGG ACAGCATTCT 
CCTAACAAGA AAAGACACAA AAAACAGGCT GCAAAAACAG AACCTGAAAA GAAGTCACAA 
TCAACTAAGC CATCTGTGGT CCATGAAAAA AAAACCCAAG AAGTAAAGCC AAAGGAACAC 
AAAGAGCCAA AAAGCCTACC CAAGCATGCA TCAGATATGG GAAGCAAGCA TGTTCATAGT 
GAAAAAGCAG CTTCTAGATC AAGTGAGCAG CTGACATCAG AGAAATCAAC AAAACCAAAG 
ACTAAAGCAC AGGATTCCGT TTCTGCCGGT GGGGAGAGTG TTGTTGCAGG TGCAGCTGCA 
GCTGCAGCAT CTGCCACAGC AGCTAACAAG TCAGACATGG ATGCTGCTTT GGATGACTTA 
GTAGACACTC TAGGAAAACC TGAAGAAACT AAAGAAGATA ATGTACCATA TACTGGACCT 
GAAATTTCAG ATCCGATGAC TTCCACCTAC ATAGAGGAAT TAGGTAAAAG AGAAGTCACA 
CTTCCTCCAA AGTACAGGGA ACTTTTGGCA AAAAAAGAAG GTATCACAGT GCCTCCTCCA 
GACTCTTCGA AACCCATGGG GCCCAATGAT GCCATAGATG CTTTGTCATC TGACTTCACC 
TGCAGTTCAC CTACCGATGC TGGAAAGAAA ACTAAGGAAG AGAAATCTAC AGGAGAAGAG 
ATTTTAAAAG CCCAGCCAGC CAGGGTAACC AAAAGTGCTG CTTCACCCCA AGAGAAAAAA 
AGAAAGGTGG AGGAGCAGGA CGCAATGAGT GATCAAGCAC TGGAGGCTCT TTCAGCTTCT 
TTGGGCAGCC GGAAGCCAGA ACCCGAGCTG GACCTCAGTT CCATTAAGGA AGTCGACGAG 
GCAAAAGCCA AAGAAGAGAA ACTAAAGAAG TGTGGTGAAG ATGAGGAAAC TGTCCCATCA 
GAGTACAGAT TAAAACCAGC TACGGATAAA GATGGAAAAC CTCTATTGCC AGAGCCTGAA 
GAAAAATCCA AGCCCCTGAG TGAATCAGAA CTCATTGATG AGCTTTCAGA AGATTTTAAC 
CAGCCTAAAA GTAAAGGAGA ACAGTCTAAG CCAACTGAAA AAACAGAAGA ATCTCAGGCT 
GCCCCCCTAA CTCCTGTGAG CGAGGCTGTG CCTCGGACCT CCATGTGTAG CATACAGTCG 
GCACCGCCCA AGCCACCTAC CTTGGAAGGC ATGGTGCCAG ATGATGCTGT AGAAGCCTTG 
GCTGGTAGCC TGGGGAAGAA GGAAGCAGAT CCAGGAGATG GAAAGCCTGT GGAAGATAAA 
GTCAAGGAGA AAGCCAAGGA AGAGGATCGT GAAAAACTTG GTGAAAAAGA AGAAACAATT 
CCTCCTGATT ATAGATTAGA AGAGGTCAAG GATAAAGATG GAAAACCACT CCTGCCAAAA 
GTGCCCAAGG AATCGCTTTT ACCCATGAGT GAAGACTTCC TTCTTGATGC TTTGTCCAAG 
GACTTTGCTG GTTCCTCAAA CACTTCACCT CTTGAATTTG AAGAGGCTAA CCTTCCTGCT 
GTCATCTCTG AAGTGGTTTC CCAAACCCCA GCTCCAACCA CGCACTCTGC TGGTCTACCC 
CCTGATACTG TGCAAAGTGA CAACAAAGAA CTGGACGATG CTTTGGACCA ACTTTCTGAC 
AGTCTGGGAC AAAGGCAGCC TGATCCAGAT GAGAACAAGC CGATAGAGGA TAAAGTCAAG 
GAAAGAGCTA AAGCTGAACA CAGAGACAAG CTGGGAGAAA GAGACGACAC CATCCCCCCT 
GAATATAGAC ACCTCTTGGA TAACGATGAG GGTAAACCAG GAAAGCCACC TGCCAAGAAA 
ACCAAGGACT CAAAGAAGCC TGAAGATGAC AAAGACCCCA TCGATGCCCT GTCAGGCGAC 
TTCGACAGCT GTCCTGCACC CACAGAAGCC TCAGAGAACA CAGCAGAGGA CAAAGCCAAG 
AGAACTGCTC CCAGCTCCAA AACACCGAAG AATAGGGGTA AAGCAAAGGA TTCTGCAAAG 
GCAAAGCAGG AAACTTCCAA GCCCAAAGCT GATGAAAAAA ATACAAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_005618082.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005618139.2 Canis lupus familiaris calpastatin (CAST), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OCe35593
Clone ID Related Accession (Same CDS sequence)	XM_005618082.3 , XM_005618082.2
Accession Version	XM_005618082.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	calpastatin isoform X15
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726052.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005618082.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCCA CAGAAACCAA GGCTATTCCA GTCAGCAAAC AGACGGGAGG ACAGCATTCT 
CCTAACAAGA AAAGACACAA AAAACAGGCT GCAAAAACAG AACCTGAAAA GAAGTCACAA 
TCAACTAAGC CATCTGTGGT CCATGAAAAA AAAACCCAAG AAGTAAAGCC AAAGGAACAC 
AAAGAGCCAA AAAGCCTACC CAAGCATGCA TCAGATATGG GAAGCAAGCA TGTTCATAGT 
GAAAAAGCAG CTTCTAGATC AAGTGAGCAG CTGACATCAG AGAAATCAAC AAAACCAAAG 
ACTAAAGCAC AGGATTCCGT TTCTGCCGGT GGGGAGAGTG TTGTTGCAGG TGCAGCTGCA 
GCTGCAGCAT CTGCCACAGC AGCTAACAAG TCAGACATGG ATGCTGCTTT GGATGACTTA 
GTAGACACTC TAGGAAAACC TGAAGAAACT AAAGAAGATA ATGTACCATA TACTGGACCT 
GAAATTTCAG ATCCGATGAC TTCCACCTAC ATAGAGGAAT TAGGTAAAAG AGAAGTCACA 
CTTCCTCCAA AGTACAGGGA ACTTTTGGCA AAAAAAGAAG GTATCACAGT GCCTCCTCCA 
GACTCTTCGA AACCCATGGG GCCCAATGAT GCCATAGATG CTTTGTCATC TGACTTCACC 
TGCAGTTCAC CTACCGATGC TGGAAAGAAA ACTAAGGAAG AGAAATCTAC AGGAGAAGAG 
ATTTTAAAAG CCCAGCCAGC CAGGGTAACC AAAAGTGCTG CTTCACCCCA AGAGAAAAAA 
AGAAAGGTGG AGGAGCAGGA CGCAATGAGT GATCAAGCAC TGGAGGCTCT TTCAGCTTCT 
TTGGGCAGCC GGAAGCCAGA ACCCGAGCTG GACCTCAGTT CCATTAAGGA AGTCGACGAG 
GCAAAAGCCA AAGAAGAGAA ACTAAAGAAG TGTGGTGAAG ATGAGGAAAC TGTCCCATCA 
GAGTACAGAT TAAAACCAGC TACGGATAAA GATGGAAAAC CTCTATTGCC AGAGCCTGAA 
GAAAAATCCA AGCCCCTGAG TGAATCAGAA CTCATTGATG AGCTTTCAGA AGATTTTAAC 
CAGCCTAAAA GTAAAGGAGA ACAGTCTAAG CCAACTGAAA AAACAGAAGA ATCTCAGGCT 
GCCCCCCTAA CTCCTGTGAG CGAGGCTGTG CCTCGGACCT CCATGTGTAG CATACAGTCG 
GCACCGCCCA AGCCACCTAC CTTGGAAGGC ATGGTGCCAG ATGATGCTGT AGAAGCCTTG 
GCTGGTAGCC TGGGGAAGAA GGAAGCAGAT CCAGGAGATG GAAAGCCTGT GGAAGATAAA 
GTCAAGGAGA AAGCCAAGGA AGAGGATCGT GAAAAACTTG GTGAAAAAGA AGAAACAATT 
CCTCCTGATT ATAGATTAGA AGAGGTCAAG GATAAAGATG GAAAACCACT CCTGCCAAAA 
GTGCCCAAGG AATCGCTTTT ACCCATGAGT GAAGACTTCC TTCTTGATGC TTTGTCCAAG 
GACTTTGCTG GTTCCTCAAA CACTTCACCT CTTGAATTTG AAGAGGCTAA CCTTCCTGCT 
GTCATCTCTG AAGTGGTTTC CCAAACCCCA GCTCCAACCA CGCACTCTGC TGGTCTACCC 
CCTGATACTG TGCAAAGTGA CAACAAAGAA CTGGACGATG CTTTGGACCA ACTTTCTGAC 
AGTCTGGGAC AAAGGCAGCC TGATCCAGAT GAGAACAAGC CGATAGAGGA TAAAGTCAAG 
GAAAGAGCTA AAGCTGAACA CAGAGACAAG CTGGGAGAAA GAGACGACAC CATCCCCCCT 
GAATATAGAC ACCTCTTGGA TAACGATGAG GGTAAACCAG GAAAGCCACC TGCCAAGAAA 
ACCAAGGACT CAAAGAAGCC TGAAGATGAC AAAGACCCCA TCGATGCCCT GTCAGGCGAC 
TTCGACAGCT GTCCTGCACC CACAGAAGCC TCAGAGAACA CAGCAGAGGA CAAAGCCAAG 
AGAACTGCTC CCAGCTCCAA AACACCGAAG AATAGGGGTA AAGCAAAGGA TTCTGCAAAG 
GCAAAGCAGG AAACTTCCAA GCCCAAAGCT GATGAAAAAA ATACAAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005618139.2
CDS	122..2212
Translation	MNPTETKAIPVSKQTGGQHSPNKKRHKKQAAKTEPEKKSQSTKPSVVHEKKTQEVKPKEHKEPKSLPKHASDMGSKHVHSEKAASRSSEQLTSEKSTKPKTKAQDSVSAGGESVVAGAAAAAASATAANKSDMDAALDDLVDTLGKPEETKEDNVPYTGPEISDPMTSTYIEELGKREVTLPPKYRELLAKKEGITVPPPDSSKPMGPNDAIDALSSDFTCSSPTDAGKKTKEEKSTGEEILKAQPARVTKSAASPQEKKRKVEEQDAMSDQALEALSASLGSRKPEPELDLSSIKEVDEAKAKEEKLKKCGEDEETVPSEYRLKPATDKDGKPLLPEPEEKSKPLSESELIDELSEDFNQPKSKGEQSKPTEKTEESQAAPLTPVSEAVPRTSMCSIQSAPPKPPTLEGMVPDDAVEALAGSLGKKEADPGDGKPVEDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKVPKESLLPMSEDFLLDALSKDFAGSSNTSPLEFEEANLPAVISEVVSQTPAPTTHSAGLPPDTVQSDNKELDDALDQLSDSLGQRQPDPDENKPIEDKVKERAKAEHRDKLGERDDTIPPEYRHLLDNDEGKPGKPPAKKTKDSKKPEDDKDPIDALSGDFDSCPAPTEASENTAEDKAKRTAPSSKTPKNRGKAKDSAKAKQETSKPKADEKNTS

Target ORF information:

RefSeq Version	XM_005618082.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calpastatin (CAST), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005618082.2

ORF Insert Sequence:

ATGAATCCCA CAGAAACCAA GGCTATTCCA GTCAGCAAAC AGACGGGAGG ACAGCATTCT 
CCTAACAAGA AAAGACACAA AAAACAGGCT GCAAAAACAG AACCTGAAAA GAAGTCACAA 
TCAACTAAGC CATCTGTGGT CCATGAAAAA AAAACCCAAG AAGTAAAGCC AAAGGAACAC 
AAAGAGCCAA AAAGCCTACC CAAGCATGCA TCAGATATGG GAAGCAAGCA TGTTCATAGT 
GAAAAAGCAG CTTCTAGATC AAGTGAGCAG CTGACATCAG AGAAATCAAC AAAACCAAAG 
ACTAAAGCAC AGGATTCCGT TTCTGCCGGT GGGGAGAGTG TTGTTGCAGG TGCAGCTGCA 
GCTGCAGCAT CTGCCACAGC AGCTAACAAG TCAGACATGG ATGCTGCTTT GGATGACTTA 
GTAGACACTC TAGGAAAACC TGAAGAAACT AAAGAAGATA ATGTACCATA TACTGGACCT 
GAAATTTCAG ATCCGATGAC TTCCACCTAC ATAGAGGAAT TAGGTAAAAG AGAAGTCACA 
CTTCCTCCAA AGTACAGGGA ACTTTTGGCA AAAAAAGAAG GTATCACAGT GCCTCCTCCA 
GACTCTTCGA AACCCATGGG GCCCAATGAT GCCATAGATG CTTTGTCATC TGACTTCACC 
TGCAGTTCAC CTACCGATGC TGGAAAGAAA ACTAAGGAAG AGAAATCTAC AGGAGAAGAG 
ATTTTAAAAG CCCAGCCAGC CAGGGTAACC AAAAGTGCTG CTTCACCCCA AGAGAAAAAA 
AGAAAGGTGG AGGAGCAGGA CGCAATGAGT GATCAAGCAC TGGAGGCTCT TTCAGCTTCT 
TTGGGCAGCC GGAAGCCAGA ACCCGAGCTG GACCTCAGTT CCATTAAGGA AGTCGACGAG 
GCAAAAGCCA AAGAAGAGAA ACTAAAGAAG TGTGGTGAAG ATGAGGAAAC TGTCCCATCA 
GAGTACAGAT TAAAACCAGC TACGGATAAA GATGGAAAAC CTCTATTGCC AGAGCCTGAA 
GAAAAATCCA AGCCCCTGAG TGAATCAGAA CTCATTGATG AGCTTTCAGA AGATTTTAAC 
CAGCCTAAAA GTAAAGGAGA ACAGTCTAAG CCAACTGAAA AAACAGAAGA ATCTCAGGCT 
GCCCCCCTAA CTCCTGTGAG CGAGGCTGTG CCTCGGACCT CCATGTGTAG CATACAGTCG 
GCACCGCCCA AGCCACCTAC CTTGGAAGGC ATGGTGCCAG ATGATGCTGT AGAAGCCTTG 
GCTGGTAGCC TGGGGAAGAA GGAAGCAGAT CCAGGAGATG GAAAGCCTGT GGAAGATAAA 
GTCAAGGAGA AAGCCAAGGA AGAGGATCGT GAAAAACTTG GTGAAAAAGA AGAAACAATT 
CCTCCTGATT ATAGATTAGA AGAGGTCAAG GATAAAGATG GAAAACCACT CCTGCCAAAA 
GTGCCCAAGG AATCGCTTTT ACCCATGAGT GAAGACTTCC TTCTTGATGC TTTGTCCAAG 
GACTTTGCTG GTTCCTCAAA CACTTCACCT CTTGAATTTG AAGAGGCTAA CCTTCCTGCT 
GTCATCTCTG AAGTGGTTTC CCAAACCCCA GCTCCAACCA CGCACTCTGC TGGTCTACCC 
CCTGATACTG TGCAAAGTGA CAACAAAGAA CTGGACGATG CTTTGGACCA ACTTTCTGAC 
AGTCTGGGAC AAAGGCAGCC TGATCCAGAT GAGAACAAGC CGATAGAGGA TAAAGTCAAG 
GAAAGAGCTA AAGCTGAACA CAGAGACAAG CTGGGAGAAA GAGACGACAC CATCCCCCCT 
GAATATAGAC ACCTCTTGGA TAACGATGAG GGTAAACCAG GAAAGCCACC TGCCAAGAAA 
ACCAAGGACT CAAAGAAGCC TGAAGATGAC AAAGACCCCA TCGATGCCCT GTCAGGCGAC 
TTCGACAGCT GTCCTGCACC CACAGAAGCC TCAGAGAACA CAGCAGAGGA CAAAGCCAAG 
AGAACTGCTC CCAGCTCCAA AACACCGAAG AATAGGGGTA AAGCAAAGGA TTCTGCAAAG 
GCAAAGCAGG AAACTTCCAA GCCCAAAGCT GATGAAAAAA ATACAAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_005618084.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005618141.2 Canis lupus familiaris calpastatin (CAST), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OCe35594
Clone ID Related Accession (Same CDS sequence)	XM_005618084.2 , XM_005618084.3
Accession Version	XM_005618084.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	calpastatin isoform X16
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726052.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005618084.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCCA CAGAAACCAA GGCTATTCCA GTCAGCAAAC AGACGGGAGG ACAGCATTCT 
CCTAACAAGA AAAGACACAA AAAACAGGCT GCAAAAACAG AACCTGAAAA GAAGTCACAA 
TCAACTAAGC CATCTGTGGT CCATGAAAAA AAAACCCAAG AAGTAAAGCC AAAGGAACAC 
AAAGAGCCAA AAAGCCTACC CAAGCATGCA TCAGATATGG GAAGCAAGCA TGTTCATAGT 
GAAAAAGCAG CTTCTAGATC AAGTGAGCAG CTGACATCAG AGAAATCAAC AAAACCAAAG 
ACTAAAGCAC AGGATTCCGT TTCTGCCGGT GGGGAGAGTG TTGTTGCAGG TGCAGCTGCA 
GCTGCAGCAT CTGCCACAGC AGCTAACAAG CAGAAAAAAG AAAAGAAATC ACTAACATCG 
GCTGTACCAG TTGAATCCAA ACCGGGTAAA CCACCTGGAA AGTCAGACAT GGATGCTGCT 
TTGGATGACT TAGTAGACAC TCTAGGAAAA CCTGAAGAAA CTAAAGAAGA TAATGTACCA 
TATACTGGAC CTGAAATTTC AGATCCGATG ACTTCCACCT ACATAGAGGA ATTAGGTAAA 
AGAGAAGTCA CACTTCCTCC AAAGTACAGG GAACTTTTGG CAAAAAAAGA AGGTATCACA 
GTGCCTCCTC CAGACTCTTC GAAACCCATG GGGCCCAATG ATGCCATAGA TGCTTTGTCA 
TCTGACTTCA CCTGCAGTTC ACCTACCGAT GCTGGAAAGA AAACTAAGGA AGAGAAATCT 
ACAGGAGAAG AGATTTTAAA AGCCCAGCCA GCCAGGGTAA CCAAAAGTGC TGCTTCACCC 
CAAGAGAAAA AAAGAAAGGT GGAGGAGCAG GACGCAATGA GTGATCAAGC ACTGGAGGCT 
CTTTCAGCTT CTTTGGGCAG CCGGAAGCCA GAACCCGAGC TGGACCTCAG TTCCATTAAG 
GAAGTCGACG AGGCAAAAGC CAAAGAAGAG AAACTAAAGA AGTGTGGTGA AGATGAGGAA 
ACTGTCCCAT CAGAGTACAG ATTAAAACCA GCTACGGATA AAGATGGAAA ACCTCTATTG 
CCAGAGCCTG AAGAAAAATC CAAGCCCCTG AGTGAATCAG AACTCATTGA TGAGCTTTCA 
GAAGATTTTA ACCAGCCTAA AAGTAAAGGA GAACAGTCTA AGCCAACTGA AAAAACAGAA 
GAATCTCAGG CTGCCCCCCT AACTCCTGTG AGCGAGGCTG TGCCTCGGAC CTCCATGTGT 
AGCATACAGT CGGCACCGCC CAAGCCACCT ACCTTGGAAG GCATGGTGCC AGATGATGCT 
GTAGAAGCCT TGGCTGGTAG CCTGGGGAAG AAGGAAGCAG ATCCAGGAGA TGGAAAGCCT 
GTGGAAGATA AAGTCAAGGA GAAAGCCAAG GAAGAGGATC GTGAAAAACT TGGTGAAAAA 
GAAGAAACAA TTCCTCCTGA TTATAGATTA GAAGAGGTCA AGGATAAAGA TGGAAAACCA 
CTCCTGCCAA AAGTGCCCAA GGAATCGCTT TTACCCATGA GTGAAGACTT CCTTCTTGAT 
GCTTTGTCCA AGGACTTTGC TGGTTCCTCA AACACTTCAC CTCTTGAATT TGAAGAGGCT 
AACCTTCCTG CTGTCATCTC TGAAGTGGTT TCCCAAACCC CAGCTCCAAC CACGCACTCT 
GCTGGTCTAC CCCCTGATAC TGTGCAAAGT GACAACAAAG AACTGGACGA TGCTTTGGAC 
CAACTTTCTG ACAGTCTGGG ACAAAGGCAG CCTGATCCAG ATGAGAACAA GCCGATAGAG 
GATAAAGTCA AGGAAAGAGC TAAAGCTGAA CACAGAGACA AGCTGGGAGA AAGAGACGAC 
ACCATCCCCC CTGAATATAG ACACCTCTTG GATAACGATG AGGGTAAACC AGGAAAGCCA 
CCTGCCAAGA AAACCAAGGA CTCAAAGAAG CCTGAAGATG ACAAAGACCC CATCGATGCC 
CTGTCAGGCG ACTTCGACAG CTGTCCTGCA CCCACAGAAG CCTCAGAGAA CACAGCAGAG 
GACAAAGCCA AGAGAACTGC TCCCAGCTCC AAAACACCGA AGAATAGGGG TAAAGCAAAG 
GATTCTGCAA AGGCAAAGCA GGAAACTTCC AAGCCCAAAG CTGATGAAAA AAATACAAGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005618141.2
CDS	148..2310
Translation	MNPTETKAIPVSKQTGGQHSPNKKRHKKQAAKTEPEKKSQSTKPSVVHEKKTQEVKPKEHKEPKSLPKHASDMGSKHVHSEKAASRSSEQLTSEKSTKPKTKAQDSVSAGGESVVAGAAAAAASATAANKQKKEKKSLTSAVPVESKPGKPPGKSDMDAALDDLVDTLGKPEETKEDNVPYTGPEISDPMTSTYIEELGKREVTLPPKYRELLAKKEGITVPPPDSSKPMGPNDAIDALSSDFTCSSPTDAGKKTKEEKSTGEEILKAQPARVTKSAASPQEKKRKVEEQDAMSDQALEALSASLGSRKPEPELDLSSIKEVDEAKAKEEKLKKCGEDEETVPSEYRLKPATDKDGKPLLPEPEEKSKPLSESELIDELSEDFNQPKSKGEQSKPTEKTEESQAAPLTPVSEAVPRTSMCSIQSAPPKPPTLEGMVPDDAVEALAGSLGKKEADPGDGKPVEDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKVPKESLLPMSEDFLLDALSKDFAGSSNTSPLEFEEANLPAVISEVVSQTPAPTTHSAGLPPDTVQSDNKELDDALDQLSDSLGQRQPDPDENKPIEDKVKERAKAEHRDKLGERDDTIPPEYRHLLDNDEGKPGKPPAKKTKDSKKPEDDKDPIDALSGDFDSCPAPTEASENTAEDKAKRTAPSSKTPKNRGKAKDSAKAKQETSKPKADEKNTS

Target ORF information:

RefSeq Version	XM_005618084.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calpastatin (CAST), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005618084.2

ORF Insert Sequence:

ATGAATCCCA CAGAAACCAA GGCTATTCCA GTCAGCAAAC AGACGGGAGG ACAGCATTCT 
CCTAACAAGA AAAGACACAA AAAACAGGCT GCAAAAACAG AACCTGAAAA GAAGTCACAA 
TCAACTAAGC CATCTGTGGT CCATGAAAAA AAAACCCAAG AAGTAAAGCC AAAGGAACAC 
AAAGAGCCAA AAAGCCTACC CAAGCATGCA TCAGATATGG GAAGCAAGCA TGTTCATAGT 
GAAAAAGCAG CTTCTAGATC AAGTGAGCAG CTGACATCAG AGAAATCAAC AAAACCAAAG 
ACTAAAGCAC AGGATTCCGT TTCTGCCGGT GGGGAGAGTG TTGTTGCAGG TGCAGCTGCA 
GCTGCAGCAT CTGCCACAGC AGCTAACAAG CAGAAAAAAG AAAAGAAATC ACTAACATCG 
GCTGTACCAG TTGAATCCAA ACCGGGTAAA CCACCTGGAA AGTCAGACAT GGATGCTGCT 
TTGGATGACT TAGTAGACAC TCTAGGAAAA CCTGAAGAAA CTAAAGAAGA TAATGTACCA 
TATACTGGAC CTGAAATTTC AGATCCGATG ACTTCCACCT ACATAGAGGA ATTAGGTAAA 
AGAGAAGTCA CACTTCCTCC AAAGTACAGG GAACTTTTGG CAAAAAAAGA AGGTATCACA 
GTGCCTCCTC CAGACTCTTC GAAACCCATG GGGCCCAATG ATGCCATAGA TGCTTTGTCA 
TCTGACTTCA CCTGCAGTTC ACCTACCGAT GCTGGAAAGA AAACTAAGGA AGAGAAATCT 
ACAGGAGAAG AGATTTTAAA AGCCCAGCCA GCCAGGGTAA CCAAAAGTGC TGCTTCACCC 
CAAGAGAAAA AAAGAAAGGT GGAGGAGCAG GACGCAATGA GTGATCAAGC ACTGGAGGCT 
CTTTCAGCTT CTTTGGGCAG CCGGAAGCCA GAACCCGAGC TGGACCTCAG TTCCATTAAG 
GAAGTCGACG AGGCAAAAGC CAAAGAAGAG AAACTAAAGA AGTGTGGTGA AGATGAGGAA 
ACTGTCCCAT CAGAGTACAG ATTAAAACCA GCTACGGATA AAGATGGAAA ACCTCTATTG 
CCAGAGCCTG AAGAAAAATC CAAGCCCCTG AGTGAATCAG AACTCATTGA TGAGCTTTCA 
GAAGATTTTA ACCAGCCTAA AAGTAAAGGA GAACAGTCTA AGCCAACTGA AAAAACAGAA 
GAATCTCAGG CTGCCCCCCT AACTCCTGTG AGCGAGGCTG TGCCTCGGAC CTCCATGTGT 
AGCATACAGT CGGCACCGCC CAAGCCACCT ACCTTGGAAG GCATGGTGCC AGATGATGCT 
GTAGAAGCCT TGGCTGGTAG CCTGGGGAAG AAGGAAGCAG ATCCAGGAGA TGGAAAGCCT 
GTGGAAGATA AAGTCAAGGA GAAAGCCAAG GAAGAGGATC GTGAAAAACT TGGTGAAAAA 
GAAGAAACAA TTCCTCCTGA TTATAGATTA GAAGAGGTCA AGGATAAAGA TGGAAAACCA 
CTCCTGCCAA AAGTGCCCAA GGAATCGCTT TTACCCATGA GTGAAGACTT CCTTCTTGAT 
GCTTTGTCCA AGGACTTTGC TGGTTCCTCA AACACTTCAC CTCTTGAATT TGAAGAGGCT 
AACCTTCCTG CTGTCATCTC TGAAGTGGTT TCCCAAACCC CAGCTCCAAC CACGCACTCT 
GCTGGTCTAC CCCCTGATAC TGTGCAAAGT GACAACAAAG AACTGGACGA TGCTTTGGAC 
CAACTTTCTG ACAGTCTGGG ACAAAGGCAG CCTGATCCAG ATGAGAACAA GCCGATAGAG 
GATAAAGTCA AGGAAAGAGC TAAAGCTGAA CACAGAGACA AGCTGGGAGA AAGAGACGAC 
ACCATCCCCC CTGAATATAG ACACCTCTTG GATAACGATG AGGGTAAACC AGGAAAGCCA 
CCTGCCAAGA AAACCAAGGA CTCAAAGAAG CCTGAAGATG ACAAAGACCC CATCGATGCC 
CTGTCAGGCG ACTTCGACAG CTGTCCTGCA CCCACAGAAG CCTCAGAGAA CACAGCAGAG 
GACAAAGCCA AGAGAACTGC TCCCAGCTCC AAAACACCGA AGAATAGGGG TAAAGCAAAG 
GATTCTGCAA AGGCAAAGCA GGAAACTTCC AAGCCCAAAG CTGATGAAAA AAATACAAGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_005618084.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005618141.2 Canis lupus familiaris calpastatin (CAST), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OCe35594
Clone ID Related Accession (Same CDS sequence)	XM_005618084.2 , XM_005618084.3
Accession Version	XM_005618084.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	calpastatin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005618084.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCCA CAGAAACCAA GGCTATTCCA GTCAGCAAAC AGACGGGAGG ACAGCATTCT 
CCTAACAAGA AAAGACACAA AAAACAGGCT GCAAAAACAG AACCTGAAAA GAAGTCACAA 
TCAACTAAGC CATCTGTGGT CCATGAAAAA AAAACCCAAG AAGTAAAGCC AAAGGAACAC 
AAAGAGCCAA AAAGCCTACC CAAGCATGCA TCAGATATGG GAAGCAAGCA TGTTCATAGT 
GAAAAAGCAG CTTCTAGATC AAGTGAGCAG CTGACATCAG AGAAATCAAC AAAACCAAAG 
ACTAAAGCAC AGGATTCCGT TTCTGCCGGT GGGGAGAGTG TTGTTGCAGG TGCAGCTGCA 
GCTGCAGCAT CTGCCACAGC AGCTAACAAG CAGAAAAAAG AAAAGAAATC ACTAACATCG 
GCTGTACCAG TTGAATCCAA ACCGGGTAAA CCACCTGGAA AGTCAGACAT GGATGCTGCT 
TTGGATGACT TAGTAGACAC TCTAGGAAAA CCTGAAGAAA CTAAAGAAGA TAATGTACCA 
TATACTGGAC CTGAAATTTC AGATCCGATG ACTTCCACCT ACATAGAGGA ATTAGGTAAA 
AGAGAAGTCA CACTTCCTCC AAAGTACAGG GAACTTTTGG CAAAAAAAGA AGGTATCACA 
GTGCCTCCTC CAGACTCTTC GAAACCCATG GGGCCCAATG ATGCCATAGA TGCTTTGTCA 
TCTGACTTCA CCTGCAGTTC ACCTACCGAT GCTGGAAAGA AAACTAAGGA AGAGAAATCT 
ACAGGAGAAG AGATTTTAAA AGCCCAGCCA GCCAGGGTAA CCAAAAGTGC TGCTTCACCC 
CAAGAGAAAA AAAGAAAGGT GGAGGAGCAG GACGCAATGA GTGATCAAGC ACTGGAGGCT 
CTTTCAGCTT CTTTGGGCAG CCGGAAGCCA GAACCCGAGC TGGACCTCAG TTCCATTAAG 
GAAGTCGACG AGGCAAAAGC CAAAGAAGAG AAACTAAAGA AGTGTGGTGA AGATGAGGAA 
ACTGTCCCAT CAGAGTACAG ATTAAAACCA GCTACGGATA AAGATGGAAA ACCTCTATTG 
CCAGAGCCTG AAGAAAAATC CAAGCCCCTG AGTGAATCAG AACTCATTGA TGAGCTTTCA 
GAAGATTTTA ACCAGCCTAA AAGTAAAGGA GAACAGTCTA AGCCAACTGA AAAAACAGAA 
GAATCTCAGG CTGCCCCCCT AACTCCTGTG AGCGAGGCTG TGCCTCGGAC CTCCATGTGT 
AGCATACAGT CGGCACCGCC CAAGCCACCT ACCTTGGAAG GCATGGTGCC AGATGATGCT 
GTAGAAGCCT TGGCTGGTAG CCTGGGGAAG AAGGAAGCAG ATCCAGGAGA TGGAAAGCCT 
GTGGAAGATA AAGTCAAGGA GAAAGCCAAG GAAGAGGATC GTGAAAAACT TGGTGAAAAA 
GAAGAAACAA TTCCTCCTGA TTATAGATTA GAAGAGGTCA AGGATAAAGA TGGAAAACCA 
CTCCTGCCAA AAGTGCCCAA GGAATCGCTT TTACCCATGA GTGAAGACTT CCTTCTTGAT 
GCTTTGTCCA AGGACTTTGC TGGTTCCTCA AACACTTCAC CTCTTGAATT TGAAGAGGCT 
AACCTTCCTG CTGTCATCTC TGAAGTGGTT TCCCAAACCC CAGCTCCAAC CACGCACTCT 
GCTGGTCTAC CCCCTGATAC TGTGCAAAGT GACAACAAAG AACTGGACGA TGCTTTGGAC 
CAACTTTCTG ACAGTCTGGG ACAAAGGCAG CCTGATCCAG ATGAGAACAA GCCGATAGAG 
GATAAAGTCA AGGAAAGAGC TAAAGCTGAA CACAGAGACA AGCTGGGAGA AAGAGACGAC 
ACCATCCCCC CTGAATATAG ACACCTCTTG GATAACGATG AGGGTAAACC AGGAAAGCCA 
CCTGCCAAGA AAACCAAGGA CTCAAAGAAG CCTGAAGATG ACAAAGACCC CATCGATGCC 
CTGTCAGGCG ACTTCGACAG CTGTCCTGCA CCCACAGAAG CCTCAGAGAA CACAGCAGAG 
GACAAAGCCA AGAGAACTGC TCCCAGCTCC AAAACACCGA AGAATAGGGG TAAAGCAAAG 
GATTCTGCAA AGGCAAAGCA GGAAACTTCC AAGCCCAAAG CTGATGAAAA AAATACAAGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005618141.2
CDS	173..2335
Translation	MNPTETKAIPVSKQTGGQHSPNKKRHKKQAAKTEPEKKSQSTKPSVVHEKKTQEVKPKEHKEPKSLPKHASDMGSKHVHSEKAASRSSEQLTSEKSTKPKTKAQDSVSAGGESVVAGAAAAAASATAANKQKKEKKSLTSAVPVESKPGKPPGKSDMDAALDDLVDTLGKPEETKEDNVPYTGPEISDPMTSTYIEELGKREVTLPPKYRELLAKKEGITVPPPDSSKPMGPNDAIDALSSDFTCSSPTDAGKKTKEEKSTGEEILKAQPARVTKSAASPQEKKRKVEEQDAMSDQALEALSASLGSRKPEPELDLSSIKEVDEAKAKEEKLKKCGEDEETVPSEYRLKPATDKDGKPLLPEPEEKSKPLSESELIDELSEDFNQPKSKGEQSKPTEKTEESQAAPLTPVSEAVPRTSMCSIQSAPPKPPTLEGMVPDDAVEALAGSLGKKEADPGDGKPVEDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKVPKESLLPMSEDFLLDALSKDFAGSSNTSPLEFEEANLPAVISEVVSQTPAPTTHSAGLPPDTVQSDNKELDDALDQLSDSLGQRQPDPDENKPIEDKVKERAKAEHRDKLGERDDTIPPEYRHLLDNDEGKPGKPPAKKTKDSKKPEDDKDPIDALSGDFDSCPAPTEASENTAEDKAKRTAPSSKTPKNRGKAKDSAKAKQETSKPKADEKNTS

Target ORF information:

RefSeq Version	XM_005618084.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calpastatin (CAST), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005618084.3

ORF Insert Sequence:

ATGAATCCCA CAGAAACCAA GGCTATTCCA GTCAGCAAAC AGACGGGAGG ACAGCATTCT 
CCTAACAAGA AAAGACACAA AAAACAGGCT GCAAAAACAG AACCTGAAAA GAAGTCACAA 
TCAACTAAGC CATCTGTGGT CCATGAAAAA AAAACCCAAG AAGTAAAGCC AAAGGAACAC 
AAAGAGCCAA AAAGCCTACC CAAGCATGCA TCAGATATGG GAAGCAAGCA TGTTCATAGT 
GAAAAAGCAG CTTCTAGATC AAGTGAGCAG CTGACATCAG AGAAATCAAC AAAACCAAAG 
ACTAAAGCAC AGGATTCCGT TTCTGCCGGT GGGGAGAGTG TTGTTGCAGG TGCAGCTGCA 
GCTGCAGCAT CTGCCACAGC AGCTAACAAG CAGAAAAAAG AAAAGAAATC ACTAACATCG 
GCTGTACCAG TTGAATCCAA ACCGGGTAAA CCACCTGGAA AGTCAGACAT GGATGCTGCT 
TTGGATGACT TAGTAGACAC TCTAGGAAAA CCTGAAGAAA CTAAAGAAGA TAATGTACCA 
TATACTGGAC CTGAAATTTC AGATCCGATG ACTTCCACCT ACATAGAGGA ATTAGGTAAA 
AGAGAAGTCA CACTTCCTCC AAAGTACAGG GAACTTTTGG CAAAAAAAGA AGGTATCACA 
GTGCCTCCTC CAGACTCTTC GAAACCCATG GGGCCCAATG ATGCCATAGA TGCTTTGTCA 
TCTGACTTCA CCTGCAGTTC ACCTACCGAT GCTGGAAAGA AAACTAAGGA AGAGAAATCT 
ACAGGAGAAG AGATTTTAAA AGCCCAGCCA GCCAGGGTAA CCAAAAGTGC TGCTTCACCC 
CAAGAGAAAA AAAGAAAGGT GGAGGAGCAG GACGCAATGA GTGATCAAGC ACTGGAGGCT 
CTTTCAGCTT CTTTGGGCAG CCGGAAGCCA GAACCCGAGC TGGACCTCAG TTCCATTAAG 
GAAGTCGACG AGGCAAAAGC CAAAGAAGAG AAACTAAAGA AGTGTGGTGA AGATGAGGAA 
ACTGTCCCAT CAGAGTACAG ATTAAAACCA GCTACGGATA AAGATGGAAA ACCTCTATTG 
CCAGAGCCTG AAGAAAAATC CAAGCCCCTG AGTGAATCAG AACTCATTGA TGAGCTTTCA 
GAAGATTTTA ACCAGCCTAA AAGTAAAGGA GAACAGTCTA AGCCAACTGA AAAAACAGAA 
GAATCTCAGG CTGCCCCCCT AACTCCTGTG AGCGAGGCTG TGCCTCGGAC CTCCATGTGT 
AGCATACAGT CGGCACCGCC CAAGCCACCT ACCTTGGAAG GCATGGTGCC AGATGATGCT 
GTAGAAGCCT TGGCTGGTAG CCTGGGGAAG AAGGAAGCAG ATCCAGGAGA TGGAAAGCCT 
GTGGAAGATA AAGTCAAGGA GAAAGCCAAG GAAGAGGATC GTGAAAAACT TGGTGAAAAA 
GAAGAAACAA TTCCTCCTGA TTATAGATTA GAAGAGGTCA AGGATAAAGA TGGAAAACCA 
CTCCTGCCAA AAGTGCCCAA GGAATCGCTT TTACCCATGA GTGAAGACTT CCTTCTTGAT 
GCTTTGTCCA AGGACTTTGC TGGTTCCTCA AACACTTCAC CTCTTGAATT TGAAGAGGCT 
AACCTTCCTG CTGTCATCTC TGAAGTGGTT TCCCAAACCC CAGCTCCAAC CACGCACTCT 
GCTGGTCTAC CCCCTGATAC TGTGCAAAGT GACAACAAAG AACTGGACGA TGCTTTGGAC 
CAACTTTCTG ACAGTCTGGG ACAAAGGCAG CCTGATCCAG ATGAGAACAA GCCGATAGAG 
GATAAAGTCA AGGAAAGAGC TAAAGCTGAA CACAGAGACA AGCTGGGAGA AAGAGACGAC 
ACCATCCCCC CTGAATATAG ACACCTCTTG GATAACGATG AGGGTAAACC AGGAAAGCCA 
CCTGCCAAGA AAACCAAGGA CTCAAAGAAG CCTGAAGATG ACAAAGACCC CATCGATGCC 
CTGTCAGGCG ACTTCGACAG CTGTCCTGCA CCCACAGAAG CCTCAGAGAA CACAGCAGAG 
GACAAAGCCA AGAGAACTGC TCCCAGCTCC AAAACACCGA AGAATAGGGG TAAAGCAAAG 
GATTCTGCAA AGGCAAAGCA GGAAACTTCC AAGCCCAAAG CTGATGAAAA AAATACAAGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_005618083.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005618140.1 Canis lupus familiaris calpastatin (CAST), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe03968
Clone ID Related Accession (Same CDS sequence)	XM_005618083.3 , XM_005618083.2
Accession Version	XM_005618083.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	calpastatin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005618083.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTATTCCAG TCAGCAAACA GACGGGAGGA 
CAGCATTCTC CTAACAAGAA AAGACACAAA AAACAGGCTG CAAAAACAGA ACCTGAAAAG 
AAGTCACAAT CAACTAAGCC ATCTGTGGTC CATGAAAAAA AAACCCAAGA AGTAAAGCCA 
AAGGAACACA AAGAGCCAAA AAGCCTACCC AAGCATGCAT CAGATATGGG AAGCAAGCAT 
GTTCATAGTG AAAAAGCAGC TTCTAGATCA AGTGAGCAGC TGACATCAGA GAAATCAACA 
AAACCAAAGA CTAAAGCACA GGATTCCGTT TCTGCCGGTG GGGAGAGTGT TGTTGCAGGT 
GCAGCTGCAG CTGCAGCATC TGCCACAGCA GCTAACAAGC AGAAAAAAGA AAAGAAATCA 
CTAACATCGG CTGTACCAGT TGAATCCAAA CCGGGTAAAC CACCTGGAAA GTCAGACATG 
GATGCTGCTT TGGATGACTT AGTAGACACT CTAGGAAAAC CTGAAGAAAC TAAAGAAGAT 
AATGTACCAT ATACTGGACC TGAAATTTCA GATCCGATGA CTTCCACCTA CATAGAGGAA 
TTAGGTAAAA GAGAAGTCAC ACTTCCTCCA AAGTACAGGG AACTTTTGGC AAAAAAAGAA 
GGTATCACAG TGCCTCCTCC AGACTCTTCG AAACCCATGG GGCCCAATGA TGCCATAGAT 
GCTTTGTCAT CTGACTTCAC CTGCAGTTCA CCTACCGATG CTGGAAAGAA AACTAAGGAA 
GAGAAATCTA CAGGAGAAGA GATTTTAAAA GCCCAGCCAG CCAGGGTAAC CAAAAGTGCT 
GCTTCACCCC AAGAGAAAAA AAGAAAGGTG GAGGAGCAGG ACGCAATGAG TGATCAAGCA 
CTGGAGGCTC TTTCAGCTTC TTTGGGCAGC CGGAAGCCAG AACCCGAGCT GGACCTCAGT 
TCCATTAAGG AAGTCGACGA GGCAAAAGCC AAAGAAGAGA AACTAAAGAA GTGTGGTGAA 
GATGAGGAAA CTGTCCCATC AGAGTACAGA TTAAAACCAG CTACGGATAA AGATGGAAAA 
CCTCTATTGC CAGAGCCTGA AGAAAAATCC AAGCCCCTGA GTGAATCAGA ACTCATTGAT 
GAGCTTTCAG AAGATTTTAA CCAGCCTAAA AGTAAAGGAG AACAGTCTAA GCCAACTGAA 
AAAACAGAAG AATCTCAGGC TGCCCCCCTA ACTCCTGTGA GCGAGGCTGT GCCTCGGACC 
TCCATGTGTA GCATACAGTC GGCACCGCCC AAGCCACCTA CCTTGGAAGG CATGGTGCCA 
GATGATGCTG TAGAAGCCTT GGCTGGTAGC CTGGGGAAGA AGGAAGCAGA TCCAGGAGAT 
GGAAAGCCTG TGGAAGATAA AGTCAAGGAG AAAGCCAAGG AAGAGGATCG TGAAAAACTT 
GGTGAAAAAG AAGAAACAAT TCCTCCTGAT TATAGATTAG AAGAGGTCAA GGATAAAGAT 
GGAAAACCAC TCCTGCCAAA AGTGCCCAAG GAATCGCTTT TACCCATGAG TGAAGACTTC 
CTTCTTGATG CTTTGTCCAA GGACTTTGCT GGTTCCTCAA ACACTTCACC TCTTGAATTT 
GAAGAGGCTA ACCTTCCTGC TGTCATCTCT GAAGTGGTTT CCCAAACCCC AGCTCCAACC 
ACGCACTCTG CTGGTCTACC CCCTGATACT GTGCAAAGTG ACAACAAAGA ACTGGACGAT 
GCTTTGGACC AACTTTCTGA CAGTCTGGGA CAAAGGCAGC CTGATCCAGA TGAGAACAAG 
CCGATAGAGG ATAAAGTCAA GGAAAGAGCT AAAGCTGAAC ACAGAGACAA GCTGGGAGAA 
AGAGACGACA CCATCCCCCC TGAATATAGA CACCTCTTGG ATAACGATGA GGGTAAACCA 
GGAAAGCCAC CTGCCAAGAA AACCAAGGAC TCAAAGAAGC CTGAAGATGA CAAAGACCCC 
ATCGATGCCC TGTCAGGCGA CTTCGACAGC TGTCCTGCAC CCACAGAAGC CTCAGAGAAC 
ACAGCAGAGG ACAAAGCCAA GAGAACTGCT CCCAGCTCCA AAACACCGAA GAATAGGGGT 
AAAGCAAAGG ATTCTGCAAA GGCAAAGCAG GAAACTTCCA AGCCCAAAGC TGATGAAAAA 
AATACAAGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005618140.1
CDS	79..2490
Translation	MSQPGQKPAASPRPRRAAAARDTQEHVSEKTSGSPSKSGEKKGADEKKARSLESSQPSRTHAGGTAPATKASASSASTSKSSSMNPTETKAIPVSKQTGGQHSPNKKRHKKQAAKTEPEKKSQSTKPSVVHEKKTQEVKPKEHKEPKSLPKHASDMGSKHVHSEKAASRSSEQLTSEKSTKPKTKAQDSVSAGGESVVAGAAAAAASATAANKQKKEKKSLTSAVPVESKPGKPPGKSDMDAALDDLVDTLGKPEETKEDNVPYTGPEISDPMTSTYIEELGKREVTLPPKYRELLAKKEGITVPPPDSSKPMGPNDAIDALSSDFTCSSPTDAGKKTKEEKSTGEEILKAQPARVTKSAASPQEKKRKVEEQDAMSDQALEALSASLGSRKPEPELDLSSIKEVDEAKAKEEKLKKCGEDEETVPSEYRLKPATDKDGKPLLPEPEEKSKPLSESELIDELSEDFNQPKSKGEQSKPTEKTEESQAAPLTPVSEAVPRTSMCSIQSAPPKPPTLEGMVPDDAVEALAGSLGKKEADPGDGKPVEDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKVPKESLLPMSEDFLLDALSKDFAGSSNTSPLEFEEANLPAVISEVVSQTPAPTTHSAGLPPDTVQSDNKELDDALDQLSDSLGQRQPDPDENKPIEDKVKERAKAEHRDKLGERDDTIPPEYRHLLDNDEGKPGKPPAKKTKDSKKPEDDKDPIDALSGDFDSCPAPTEASENTAEDKAKRTAPSSKTPKNRGKAKDSAKAKQETSKPKADEKNTS

Target ORF information:

RefSeq Version	XM_005618083.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calpastatin (CAST), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005618083.3

ORF Insert Sequence:

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTATTCCAG TCAGCAAACA GACGGGAGGA 
CAGCATTCTC CTAACAAGAA AAGACACAAA AAACAGGCTG CAAAAACAGA ACCTGAAAAG 
AAGTCACAAT CAACTAAGCC ATCTGTGGTC CATGAAAAAA AAACCCAAGA AGTAAAGCCA 
AAGGAACACA AAGAGCCAAA AAGCCTACCC AAGCATGCAT CAGATATGGG AAGCAAGCAT 
GTTCATAGTG AAAAAGCAGC TTCTAGATCA AGTGAGCAGC TGACATCAGA GAAATCAACA 
AAACCAAAGA CTAAAGCACA GGATTCCGTT TCTGCCGGTG GGGAGAGTGT TGTTGCAGGT 
GCAGCTGCAG CTGCAGCATC TGCCACAGCA GCTAACAAGC AGAAAAAAGA AAAGAAATCA 
CTAACATCGG CTGTACCAGT TGAATCCAAA CCGGGTAAAC CACCTGGAAA GTCAGACATG 
GATGCTGCTT TGGATGACTT AGTAGACACT CTAGGAAAAC CTGAAGAAAC TAAAGAAGAT 
AATGTACCAT ATACTGGACC TGAAATTTCA GATCCGATGA CTTCCACCTA CATAGAGGAA 
TTAGGTAAAA GAGAAGTCAC ACTTCCTCCA AAGTACAGGG AACTTTTGGC AAAAAAAGAA 
GGTATCACAG TGCCTCCTCC AGACTCTTCG AAACCCATGG GGCCCAATGA TGCCATAGAT 
GCTTTGTCAT CTGACTTCAC CTGCAGTTCA CCTACCGATG CTGGAAAGAA AACTAAGGAA 
GAGAAATCTA CAGGAGAAGA GATTTTAAAA GCCCAGCCAG CCAGGGTAAC CAAAAGTGCT 
GCTTCACCCC AAGAGAAAAA AAGAAAGGTG GAGGAGCAGG ACGCAATGAG TGATCAAGCA 
CTGGAGGCTC TTTCAGCTTC TTTGGGCAGC CGGAAGCCAG AACCCGAGCT GGACCTCAGT 
TCCATTAAGG AAGTCGACGA GGCAAAAGCC AAAGAAGAGA AACTAAAGAA GTGTGGTGAA 
GATGAGGAAA CTGTCCCATC AGAGTACAGA TTAAAACCAG CTACGGATAA AGATGGAAAA 
CCTCTATTGC CAGAGCCTGA AGAAAAATCC AAGCCCCTGA GTGAATCAGA ACTCATTGAT 
GAGCTTTCAG AAGATTTTAA CCAGCCTAAA AGTAAAGGAG AACAGTCTAA GCCAACTGAA 
AAAACAGAAG AATCTCAGGC TGCCCCCCTA ACTCCTGTGA GCGAGGCTGT GCCTCGGACC 
TCCATGTGTA GCATACAGTC GGCACCGCCC AAGCCACCTA CCTTGGAAGG CATGGTGCCA 
GATGATGCTG TAGAAGCCTT GGCTGGTAGC CTGGGGAAGA AGGAAGCAGA TCCAGGAGAT 
GGAAAGCCTG TGGAAGATAA AGTCAAGGAG AAAGCCAAGG AAGAGGATCG TGAAAAACTT 
GGTGAAAAAG AAGAAACAAT TCCTCCTGAT TATAGATTAG AAGAGGTCAA GGATAAAGAT 
GGAAAACCAC TCCTGCCAAA AGTGCCCAAG GAATCGCTTT TACCCATGAG TGAAGACTTC 
CTTCTTGATG CTTTGTCCAA GGACTTTGCT GGTTCCTCAA ACACTTCACC TCTTGAATTT 
GAAGAGGCTA ACCTTCCTGC TGTCATCTCT GAAGTGGTTT CCCAAACCCC AGCTCCAACC 
ACGCACTCTG CTGGTCTACC CCCTGATACT GTGCAAAGTG ACAACAAAGA ACTGGACGAT 
GCTTTGGACC AACTTTCTGA CAGTCTGGGA CAAAGGCAGC CTGATCCAGA TGAGAACAAG 
CCGATAGAGG ATAAAGTCAA GGAAAGAGCT AAAGCTGAAC ACAGAGACAA GCTGGGAGAA 
AGAGACGACA CCATCCCCCC TGAATATAGA CACCTCTTGG ATAACGATGA GGGTAAACCA 
GGAAAGCCAC CTGCCAAGAA AACCAAGGAC TCAAAGAAGC CTGAAGATGA CAAAGACCCC 
ATCGATGCCC TGTCAGGCGA CTTCGACAGC TGTCCTGCAC CCACAGAAGC CTCAGAGAAC 
ACAGCAGAGG ACAAAGCCAA GAGAACTGCT CCCAGCTCCA AAACACCGAA GAATAGGGGT 
AAAGCAAAGG ATTCTGCAAA GGCAAAGCAG GAAACTTCCA AGCCCAAAGC TGATGAAAAA 
AATACAAGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_005618083.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005618140.1 Canis lupus familiaris calpastatin (CAST), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe03968
Clone ID Related Accession (Same CDS sequence)	XM_005618083.3 , XM_005618083.2
Accession Version	XM_005618083.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	calpastatin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726052.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005618083.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTATTCCAG TCAGCAAACA GACGGGAGGA 
CAGCATTCTC CTAACAAGAA AAGACACAAA AAACAGGCTG CAAAAACAGA ACCTGAAAAG 
AAGTCACAAT CAACTAAGCC ATCTGTGGTC CATGAAAAAA AAACCCAAGA AGTAAAGCCA 
AAGGAACACA AAGAGCCAAA AAGCCTACCC AAGCATGCAT CAGATATGGG AAGCAAGCAT 
GTTCATAGTG AAAAAGCAGC TTCTAGATCA AGTGAGCAGC TGACATCAGA GAAATCAACA 
AAACCAAAGA CTAAAGCACA GGATTCCGTT TCTGCCGGTG GGGAGAGTGT TGTTGCAGGT 
GCAGCTGCAG CTGCAGCATC TGCCACAGCA GCTAACAAGC AGAAAAAAGA AAAGAAATCA 
CTAACATCGG CTGTACCAGT TGAATCCAAA CCGGGTAAAC CACCTGGAAA GTCAGACATG 
GATGCTGCTT TGGATGACTT AGTAGACACT CTAGGAAAAC CTGAAGAAAC TAAAGAAGAT 
AATGTACCAT ATACTGGACC TGAAATTTCA GATCCGATGA CTTCCACCTA CATAGAGGAA 
TTAGGTAAAA GAGAAGTCAC ACTTCCTCCA AAGTACAGGG AACTTTTGGC AAAAAAAGAA 
GGTATCACAG TGCCTCCTCC AGACTCTTCG AAACCCATGG GGCCCAATGA TGCCATAGAT 
GCTTTGTCAT CTGACTTCAC CTGCAGTTCA CCTACCGATG CTGGAAAGAA AACTAAGGAA 
GAGAAATCTA CAGGAGAAGA GATTTTAAAA GCCCAGCCAG CCAGGGTAAC CAAAAGTGCT 
GCTTCACCCC AAGAGAAAAA AAGAAAGGTG GAGGAGCAGG ACGCAATGAG TGATCAAGCA 
CTGGAGGCTC TTTCAGCTTC TTTGGGCAGC CGGAAGCCAG AACCCGAGCT GGACCTCAGT 
TCCATTAAGG AAGTCGACGA GGCAAAAGCC AAAGAAGAGA AACTAAAGAA GTGTGGTGAA 
GATGAGGAAA CTGTCCCATC AGAGTACAGA TTAAAACCAG CTACGGATAA AGATGGAAAA 
CCTCTATTGC CAGAGCCTGA AGAAAAATCC AAGCCCCTGA GTGAATCAGA ACTCATTGAT 
GAGCTTTCAG AAGATTTTAA CCAGCCTAAA AGTAAAGGAG AACAGTCTAA GCCAACTGAA 
AAAACAGAAG AATCTCAGGC TGCCCCCCTA ACTCCTGTGA GCGAGGCTGT GCCTCGGACC 
TCCATGTGTA GCATACAGTC GGCACCGCCC AAGCCACCTA CCTTGGAAGG CATGGTGCCA 
GATGATGCTG TAGAAGCCTT GGCTGGTAGC CTGGGGAAGA AGGAAGCAGA TCCAGGAGAT 
GGAAAGCCTG TGGAAGATAA AGTCAAGGAG AAAGCCAAGG AAGAGGATCG TGAAAAACTT 
GGTGAAAAAG AAGAAACAAT TCCTCCTGAT TATAGATTAG AAGAGGTCAA GGATAAAGAT 
GGAAAACCAC TCCTGCCAAA AGTGCCCAAG GAATCGCTTT TACCCATGAG TGAAGACTTC 
CTTCTTGATG CTTTGTCCAA GGACTTTGCT GGTTCCTCAA ACACTTCACC TCTTGAATTT 
GAAGAGGCTA ACCTTCCTGC TGTCATCTCT GAAGTGGTTT CCCAAACCCC AGCTCCAACC 
ACGCACTCTG CTGGTCTACC CCCTGATACT GTGCAAAGTG ACAACAAAGA ACTGGACGAT 
GCTTTGGACC AACTTTCTGA CAGTCTGGGA CAAAGGCAGC CTGATCCAGA TGAGAACAAG 
CCGATAGAGG ATAAAGTCAA GGAAAGAGCT AAAGCTGAAC ACAGAGACAA GCTGGGAGAA 
AGAGACGACA CCATCCCCCC TGAATATAGA CACCTCTTGG ATAACGATGA GGGTAAACCA 
GGAAAGCCAC CTGCCAAGAA AACCAAGGAC TCAAAGAAGC CTGAAGATGA CAAAGACCCC 
ATCGATGCCC TGTCAGGCGA CTTCGACAGC TGTCCTGCAC CCACAGAAGC CTCAGAGAAC 
ACAGCAGAGG ACAAAGCCAA GAGAACTGCT CCCAGCTCCA AAACACCGAA GAATAGGGGT 
AAAGCAAAGG ATTCTGCAAA GGCAAAGCAG GAAACTTCCA AGCCCAAAGC TGATGAAAAA 
AATACAAGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005618140.1
CDS	102..2513
Translation	MSQPGQKPAASPRPRRAAAARDTQEHVSEKTSGSPSKSGEKKGADEKKARSLESSQPSRTHAGGTAPATKASASSASTSKSSSMNPTETKAIPVSKQTGGQHSPNKKRHKKQAAKTEPEKKSQSTKPSVVHEKKTQEVKPKEHKEPKSLPKHASDMGSKHVHSEKAASRSSEQLTSEKSTKPKTKAQDSVSAGGESVVAGAAAAAASATAANKQKKEKKSLTSAVPVESKPGKPPGKSDMDAALDDLVDTLGKPEETKEDNVPYTGPEISDPMTSTYIEELGKREVTLPPKYRELLAKKEGITVPPPDSSKPMGPNDAIDALSSDFTCSSPTDAGKKTKEEKSTGEEILKAQPARVTKSAASPQEKKRKVEEQDAMSDQALEALSASLGSRKPEPELDLSSIKEVDEAKAKEEKLKKCGEDEETVPSEYRLKPATDKDGKPLLPEPEEKSKPLSESELIDELSEDFNQPKSKGEQSKPTEKTEESQAAPLTPVSEAVPRTSMCSIQSAPPKPPTLEGMVPDDAVEALAGSLGKKEADPGDGKPVEDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKVPKESLLPMSEDFLLDALSKDFAGSSNTSPLEFEEANLPAVISEVVSQTPAPTTHSAGLPPDTVQSDNKELDDALDQLSDSLGQRQPDPDENKPIEDKVKERAKAEHRDKLGERDDTIPPEYRHLLDNDEGKPGKPPAKKTKDSKKPEDDKDPIDALSGDFDSCPAPTEASENTAEDKAKRTAPSSKTPKNRGKAKDSAKAKQETSKPKADEKNTS

Target ORF information:

RefSeq Version	XM_005618083.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calpastatin (CAST), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005618083.2

ORF Insert Sequence:

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTATTCCAG TCAGCAAACA GACGGGAGGA 
CAGCATTCTC CTAACAAGAA AAGACACAAA AAACAGGCTG CAAAAACAGA ACCTGAAAAG 
AAGTCACAAT CAACTAAGCC ATCTGTGGTC CATGAAAAAA AAACCCAAGA AGTAAAGCCA 
AAGGAACACA AAGAGCCAAA AAGCCTACCC AAGCATGCAT CAGATATGGG AAGCAAGCAT 
GTTCATAGTG AAAAAGCAGC TTCTAGATCA AGTGAGCAGC TGACATCAGA GAAATCAACA 
AAACCAAAGA CTAAAGCACA GGATTCCGTT TCTGCCGGTG GGGAGAGTGT TGTTGCAGGT 
GCAGCTGCAG CTGCAGCATC TGCCACAGCA GCTAACAAGC AGAAAAAAGA AAAGAAATCA 
CTAACATCGG CTGTACCAGT TGAATCCAAA CCGGGTAAAC CACCTGGAAA GTCAGACATG 
GATGCTGCTT TGGATGACTT AGTAGACACT CTAGGAAAAC CTGAAGAAAC TAAAGAAGAT 
AATGTACCAT ATACTGGACC TGAAATTTCA GATCCGATGA CTTCCACCTA CATAGAGGAA 
TTAGGTAAAA GAGAAGTCAC ACTTCCTCCA AAGTACAGGG AACTTTTGGC AAAAAAAGAA 
GGTATCACAG TGCCTCCTCC AGACTCTTCG AAACCCATGG GGCCCAATGA TGCCATAGAT 
GCTTTGTCAT CTGACTTCAC CTGCAGTTCA CCTACCGATG CTGGAAAGAA AACTAAGGAA 
GAGAAATCTA CAGGAGAAGA GATTTTAAAA GCCCAGCCAG CCAGGGTAAC CAAAAGTGCT 
GCTTCACCCC AAGAGAAAAA AAGAAAGGTG GAGGAGCAGG ACGCAATGAG TGATCAAGCA 
CTGGAGGCTC TTTCAGCTTC TTTGGGCAGC CGGAAGCCAG AACCCGAGCT GGACCTCAGT 
TCCATTAAGG AAGTCGACGA GGCAAAAGCC AAAGAAGAGA AACTAAAGAA GTGTGGTGAA 
GATGAGGAAA CTGTCCCATC AGAGTACAGA TTAAAACCAG CTACGGATAA AGATGGAAAA 
CCTCTATTGC CAGAGCCTGA AGAAAAATCC AAGCCCCTGA GTGAATCAGA ACTCATTGAT 
GAGCTTTCAG AAGATTTTAA CCAGCCTAAA AGTAAAGGAG AACAGTCTAA GCCAACTGAA 
AAAACAGAAG AATCTCAGGC TGCCCCCCTA ACTCCTGTGA GCGAGGCTGT GCCTCGGACC 
TCCATGTGTA GCATACAGTC GGCACCGCCC AAGCCACCTA CCTTGGAAGG CATGGTGCCA 
GATGATGCTG TAGAAGCCTT GGCTGGTAGC CTGGGGAAGA AGGAAGCAGA TCCAGGAGAT 
GGAAAGCCTG TGGAAGATAA AGTCAAGGAG AAAGCCAAGG AAGAGGATCG TGAAAAACTT 
GGTGAAAAAG AAGAAACAAT TCCTCCTGAT TATAGATTAG AAGAGGTCAA GGATAAAGAT 
GGAAAACCAC TCCTGCCAAA AGTGCCCAAG GAATCGCTTT TACCCATGAG TGAAGACTTC 
CTTCTTGATG CTTTGTCCAA GGACTTTGCT GGTTCCTCAA ACACTTCACC TCTTGAATTT 
GAAGAGGCTA ACCTTCCTGC TGTCATCTCT GAAGTGGTTT CCCAAACCCC AGCTCCAACC 
ACGCACTCTG CTGGTCTACC CCCTGATACT GTGCAAAGTG ACAACAAAGA ACTGGACGAT 
GCTTTGGACC AACTTTCTGA CAGTCTGGGA CAAAGGCAGC CTGATCCAGA TGAGAACAAG 
CCGATAGAGG ATAAAGTCAA GGAAAGAGCT AAAGCTGAAC ACAGAGACAA GCTGGGAGAA 
AGAGACGACA CCATCCCCCC TGAATATAGA CACCTCTTGG ATAACGATGA GGGTAAACCA 
GGAAAGCCAC CTGCCAAGAA AACCAAGGAC TCAAAGAAGC CTGAAGATGA CAAAGACCCC 
ATCGATGCCC TGTCAGGCGA CTTCGACAGC TGTCCTGCAC CCACAGAAGC CTCAGAGAAC 
ACAGCAGAGG ACAAAGCCAA GAGAACTGCT CCCAGCTCCA AAACACCGAA GAATAGGGGT 
AAAGCAAAGG ATTCTGCAAA GGCAAAGCAG GAAACTTCCA AGCCCAAAGC TGATGAAAAA 
AATACAAGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_843528.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_848621.3 Canis lupus familiaris calpastatin (CAST), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe03969
Clone ID Related Accession (Same CDS sequence)	XM_843528.5 , XM_843528.4
Accession Version	XM_843528.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	calpastatin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_843528.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTATTCCAG TCAGCAAACA GACGGGAGGA 
CAGCATTCTC CTAACAAGAA AAGACACAAA AAACAGGCTG CAAAAACAGA ACCTGAAAAG 
AAGTCACAAT CAACTAAGCC ATCTGTGGTC CATGAAAAAA AAACCCAAGA AGTAAAGCCA 
AAGGAACACA AAGAGCCAAA AAGCCTACCC AAGCATGCAT CAGATATGGG AAGCAAGCAT 
GTTCATAGTG AAAAAGCAGC TTCTAGATCA AGTGAGCAGC TGACATCAGA GAAATCAACA 
AAACCAAAGA CTAAAGCACA GGATTCCGTT TCTGCCGGTG GGGAGAGTGT TGTTGCAGGT 
GCAGCTGCAG CTGCAGCATC TGCCACAGCA GCTAACAAGA AAAAAGAAAA GAAATCACTA 
ACATCGGCTG TACCAGTTGA ATCCAAACCG GGTAAACCAC CTGGAAAGTC AGACATGGAT 
GCTGCTTTGG ATGACTTAGT AGACACTCTA GGAAAACCTG AAGAAACTAA AGAAGATAAT 
GTACCATATA CTGGACCTGA AATTTCAGAT CCGATGACTT CCACCTACAT AGAGGAATTA 
GGTAAAAGAG AAGTCACACT TCCTCCAAAG TACAGGGAAC TTTTGGCAAA AAAAGAAGGT 
ATCACAGTGC CTCCTCCAGA CTCTTCGAAA CCCATGGGGC CCAATGATGC CATAGATGCT 
TTGTCATCTG ACTTCACCTG CAGTTCACCT ACCGATGCTG GAAAGAAAAC TAAGGAAGAG 
AAATCTACAG GAGAAGAGAT TTTAAAAGCC CAGCCAGCCA GGGTAACCAA AAGTGCTGCT 
TCACCCCAAG AGAAAAAAAG AAAGGTGGAG GAGGACGCAA TGAGTGATCA AGCACTGGAG 
GCTCTTTCAG CTTCTTTGGG CAGCCGGAAG CCAGAACCCG AGCTGGACCT CAGTTCCATT 
AAGGAAGTCG ACGAGGCAAA AGCCAAAGAA GAGAAACTAA AGAAGTGTGG TGAAGATGAG 
GAAACTGTCC CATCAGAGTA CAGATTAAAA CCAGCTACGG ATAAAGATGG AAAACCTCTA 
TTGCCAGAGC CTGAAGAAAA ATCCAAGCCC CTGAGTGAAT CAGAACTCAT TGATGAGCTT 
TCAGAAGATT TTAACCAGCC TAAAAGTAAA GGAGAACAGT CTAAGCCAAC TGAAAAAACA 
GAAGAATCTC AGGCTGCCCC CCTAACTCCT GTGAGCGAGG CTGTGCCTCG GACCTCCATG 
TGTAGCATAC AGTCGGCACC GCCCAAGCCA CCTACCTTGG AAGGCATGGT GCCAGATGAT 
GCTGTAGAAG CCTTGGCTGG TAGCCTGGGG AAGAAGGAAG CAGATCCAGG AGATGGAAAG 
CCTGTGGAAG ATAAAGTCAA GGAGAAAGCC AAGGAAGAGG ATCGTGAAAA ACTTGGTGAA 
AAAGAAGAAA CAATTCCTCC TGATTATAGA TTAGAAGAGG TCAAGGATAA AGATGGAAAA 
CCACTCCTGC CAAAAGTGCC CAAGGAATCG CTTTTACCCA TGAGTGAAGA CTTCCTTCTT 
GATGCTTTGT CCAAGGACTT TGCTGGTTCC TCAAACACTT CACCTCTTGA ATTTGAAGAG 
GCTAACCTTC CTGCTGTCAT CTCTGAAGTG GTTTCCCAAA CCCCAGCTCC AACCACGCAC 
TCTGCTGGTC TACCCCCTGA TACTGTGCAA AGTGACAACA AAGAACTGGA CGATGCTTTG 
GACCAACTTT CTGACAGTCT GGGACAAAGG CAGCCTGATC CAGATGAGAA CAAGCCGATA 
GAGGATAAAG TCAAGGAAAG AGCTAAAGCT GAACACAGAG ACAAGCTGGG AGAAAGAGAC 
GACACCATCC CCCCTGAATA TAGACACCTC TTGGATAACG ATGAGGGTAA ACCAGGAAAG 
CCACCTGCCA AGAAAACCAA GGACTCAAAG AAGCCTGAAG ATGACAAAGA CCCCATCGAT 
GCCCTGTCAG GCGACTTCGA CAGCTGTCCT GCACCCACAG AAGCCTCAGA GAACACAGCA 
GAGGACAAAG CCAAGAGAAC TGCTCCCAGC TCCAAAACAC CGAAGAATAG GGGTAAAGCA 
AAGGATTCTG CAAAGGCAAA GCAGGAAACT TCCAAGCCCA AAGCTGATGA AAAAAATACA 
AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_848621.3
CDS	79..2484
Translation	MSQPGQKPAASPRPRRAAAARDTQEHVSEKTSGSPSKSGEKKGADEKKARSLESSQPSRTHAGGTAPATKASASSASTSKSSSMNPTETKAIPVSKQTGGQHSPNKKRHKKQAAKTEPEKKSQSTKPSVVHEKKTQEVKPKEHKEPKSLPKHASDMGSKHVHSEKAASRSSEQLTSEKSTKPKTKAQDSVSAGGESVVAGAAAAAASATAANKKKEKKSLTSAVPVESKPGKPPGKSDMDAALDDLVDTLGKPEETKEDNVPYTGPEISDPMTSTYIEELGKREVTLPPKYRELLAKKEGITVPPPDSSKPMGPNDAIDALSSDFTCSSPTDAGKKTKEEKSTGEEILKAQPARVTKSAASPQEKKRKVEEDAMSDQALEALSASLGSRKPEPELDLSSIKEVDEAKAKEEKLKKCGEDEETVPSEYRLKPATDKDGKPLLPEPEEKSKPLSESELIDELSEDFNQPKSKGEQSKPTEKTEESQAAPLTPVSEAVPRTSMCSIQSAPPKPPTLEGMVPDDAVEALAGSLGKKEADPGDGKPVEDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKVPKESLLPMSEDFLLDALSKDFAGSSNTSPLEFEEANLPAVISEVVSQTPAPTTHSAGLPPDTVQSDNKELDDALDQLSDSLGQRQPDPDENKPIEDKVKERAKAEHRDKLGERDDTIPPEYRHLLDNDEGKPGKPPAKKTKDSKKPEDDKDPIDALSGDFDSCPAPTEASENTAEDKAKRTAPSSKTPKNRGKAKDSAKAKQETSKPKADEKNTS

Target ORF information:

RefSeq Version	XM_843528.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calpastatin (CAST), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_843528.5

ORF Insert Sequence:

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTATTCCAG TCAGCAAACA GACGGGAGGA 
CAGCATTCTC CTAACAAGAA AAGACACAAA AAACAGGCTG CAAAAACAGA ACCTGAAAAG 
AAGTCACAAT CAACTAAGCC ATCTGTGGTC CATGAAAAAA AAACCCAAGA AGTAAAGCCA 
AAGGAACACA AAGAGCCAAA AAGCCTACCC AAGCATGCAT CAGATATGGG AAGCAAGCAT 
GTTCATAGTG AAAAAGCAGC TTCTAGATCA AGTGAGCAGC TGACATCAGA GAAATCAACA 
AAACCAAAGA CTAAAGCACA GGATTCCGTT TCTGCCGGTG GGGAGAGTGT TGTTGCAGGT 
GCAGCTGCAG CTGCAGCATC TGCCACAGCA GCTAACAAGA AAAAAGAAAA GAAATCACTA 
ACATCGGCTG TACCAGTTGA ATCCAAACCG GGTAAACCAC CTGGAAAGTC AGACATGGAT 
GCTGCTTTGG ATGACTTAGT AGACACTCTA GGAAAACCTG AAGAAACTAA AGAAGATAAT 
GTACCATATA CTGGACCTGA AATTTCAGAT CCGATGACTT CCACCTACAT AGAGGAATTA 
GGTAAAAGAG AAGTCACACT TCCTCCAAAG TACAGGGAAC TTTTGGCAAA AAAAGAAGGT 
ATCACAGTGC CTCCTCCAGA CTCTTCGAAA CCCATGGGGC CCAATGATGC CATAGATGCT 
TTGTCATCTG ACTTCACCTG CAGTTCACCT ACCGATGCTG GAAAGAAAAC TAAGGAAGAG 
AAATCTACAG GAGAAGAGAT TTTAAAAGCC CAGCCAGCCA GGGTAACCAA AAGTGCTGCT 
TCACCCCAAG AGAAAAAAAG AAAGGTGGAG GAGGACGCAA TGAGTGATCA AGCACTGGAG 
GCTCTTTCAG CTTCTTTGGG CAGCCGGAAG CCAGAACCCG AGCTGGACCT CAGTTCCATT 
AAGGAAGTCG ACGAGGCAAA AGCCAAAGAA GAGAAACTAA AGAAGTGTGG TGAAGATGAG 
GAAACTGTCC CATCAGAGTA CAGATTAAAA CCAGCTACGG ATAAAGATGG AAAACCTCTA 
TTGCCAGAGC CTGAAGAAAA ATCCAAGCCC CTGAGTGAAT CAGAACTCAT TGATGAGCTT 
TCAGAAGATT TTAACCAGCC TAAAAGTAAA GGAGAACAGT CTAAGCCAAC TGAAAAAACA 
GAAGAATCTC AGGCTGCCCC CCTAACTCCT GTGAGCGAGG CTGTGCCTCG GACCTCCATG 
TGTAGCATAC AGTCGGCACC GCCCAAGCCA CCTACCTTGG AAGGCATGGT GCCAGATGAT 
GCTGTAGAAG CCTTGGCTGG TAGCCTGGGG AAGAAGGAAG CAGATCCAGG AGATGGAAAG 
CCTGTGGAAG ATAAAGTCAA GGAGAAAGCC AAGGAAGAGG ATCGTGAAAA ACTTGGTGAA 
AAAGAAGAAA CAATTCCTCC TGATTATAGA TTAGAAGAGG TCAAGGATAA AGATGGAAAA 
CCACTCCTGC CAAAAGTGCC CAAGGAATCG CTTTTACCCA TGAGTGAAGA CTTCCTTCTT 
GATGCTTTGT CCAAGGACTT TGCTGGTTCC TCAAACACTT CACCTCTTGA ATTTGAAGAG 
GCTAACCTTC CTGCTGTCAT CTCTGAAGTG GTTTCCCAAA CCCCAGCTCC AACCACGCAC 
TCTGCTGGTC TACCCCCTGA TACTGTGCAA AGTGACAACA AAGAACTGGA CGATGCTTTG 
GACCAACTTT CTGACAGTCT GGGACAAAGG CAGCCTGATC CAGATGAGAA CAAGCCGATA 
GAGGATAAAG TCAAGGAAAG AGCTAAAGCT GAACACAGAG ACAAGCTGGG AGAAAGAGAC 
GACACCATCC CCCCTGAATA TAGACACCTC TTGGATAACG ATGAGGGTAA ACCAGGAAAG 
CCACCTGCCA AGAAAACCAA GGACTCAAAG AAGCCTGAAG ATGACAAAGA CCCCATCGAT 
GCCCTGTCAG GCGACTTCGA CAGCTGTCCT GCACCCACAG AAGCCTCAGA GAACACAGCA 
GAGGACAAAG CCAAGAGAAC TGCTCCCAGC TCCAAAACAC CGAAGAATAG GGGTAAAGCA 
AAGGATTCTG CAAAGGCAAA GCAGGAAACT TCCAAGCCCA AAGCTGATGA AAAAAATACA 
AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_843528.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_848621.3 Canis lupus familiaris calpastatin (CAST), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe03969
Clone ID Related Accession (Same CDS sequence)	XM_843528.5 , XM_843528.4
Accession Version	XM_843528.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	calpastatin isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726052.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_843528.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTATTCCAG TCAGCAAACA GACGGGAGGA 
CAGCATTCTC CTAACAAGAA AAGACACAAA AAACAGGCTG CAAAAACAGA ACCTGAAAAG 
AAGTCACAAT CAACTAAGCC ATCTGTGGTC CATGAAAAAA AAACCCAAGA AGTAAAGCCA 
AAGGAACACA AAGAGCCAAA AAGCCTACCC AAGCATGCAT CAGATATGGG AAGCAAGCAT 
GTTCATAGTG AAAAAGCAGC TTCTAGATCA AGTGAGCAGC TGACATCAGA GAAATCAACA 
AAACCAAAGA CTAAAGCACA GGATTCCGTT TCTGCCGGTG GGGAGAGTGT TGTTGCAGGT 
GCAGCTGCAG CTGCAGCATC TGCCACAGCA GCTAACAAGA AAAAAGAAAA GAAATCACTA 
ACATCGGCTG TACCAGTTGA ATCCAAACCG GGTAAACCAC CTGGAAAGTC AGACATGGAT 
GCTGCTTTGG ATGACTTAGT AGACACTCTA GGAAAACCTG AAGAAACTAA AGAAGATAAT 
GTACCATATA CTGGACCTGA AATTTCAGAT CCGATGACTT CCACCTACAT AGAGGAATTA 
GGTAAAAGAG AAGTCACACT TCCTCCAAAG TACAGGGAAC TTTTGGCAAA AAAAGAAGGT 
ATCACAGTGC CTCCTCCAGA CTCTTCGAAA CCCATGGGGC CCAATGATGC CATAGATGCT 
TTGTCATCTG ACTTCACCTG CAGTTCACCT ACCGATGCTG GAAAGAAAAC TAAGGAAGAG 
AAATCTACAG GAGAAGAGAT TTTAAAAGCC CAGCCAGCCA GGGTAACCAA AAGTGCTGCT 
TCACCCCAAG AGAAAAAAAG AAAGGTGGAG GAGGACGCAA TGAGTGATCA AGCACTGGAG 
GCTCTTTCAG CTTCTTTGGG CAGCCGGAAG CCAGAACCCG AGCTGGACCT CAGTTCCATT 
AAGGAAGTCG ACGAGGCAAA AGCCAAAGAA GAGAAACTAA AGAAGTGTGG TGAAGATGAG 
GAAACTGTCC CATCAGAGTA CAGATTAAAA CCAGCTACGG ATAAAGATGG AAAACCTCTA 
TTGCCAGAGC CTGAAGAAAA ATCCAAGCCC CTGAGTGAAT CAGAACTCAT TGATGAGCTT 
TCAGAAGATT TTAACCAGCC TAAAAGTAAA GGAGAACAGT CTAAGCCAAC TGAAAAAACA 
GAAGAATCTC AGGCTGCCCC CCTAACTCCT GTGAGCGAGG CTGTGCCTCG GACCTCCATG 
TGTAGCATAC AGTCGGCACC GCCCAAGCCA CCTACCTTGG AAGGCATGGT GCCAGATGAT 
GCTGTAGAAG CCTTGGCTGG TAGCCTGGGG AAGAAGGAAG CAGATCCAGG AGATGGAAAG 
CCTGTGGAAG ATAAAGTCAA GGAGAAAGCC AAGGAAGAGG ATCGTGAAAA ACTTGGTGAA 
AAAGAAGAAA CAATTCCTCC TGATTATAGA TTAGAAGAGG TCAAGGATAA AGATGGAAAA 
CCACTCCTGC CAAAAGTGCC CAAGGAATCG CTTTTACCCA TGAGTGAAGA CTTCCTTCTT 
GATGCTTTGT CCAAGGACTT TGCTGGTTCC TCAAACACTT CACCTCTTGA ATTTGAAGAG 
GCTAACCTTC CTGCTGTCAT CTCTGAAGTG GTTTCCCAAA CCCCAGCTCC AACCACGCAC 
TCTGCTGGTC TACCCCCTGA TACTGTGCAA AGTGACAACA AAGAACTGGA CGATGCTTTG 
GACCAACTTT CTGACAGTCT GGGACAAAGG CAGCCTGATC CAGATGAGAA CAAGCCGATA 
GAGGATAAAG TCAAGGAAAG AGCTAAAGCT GAACACAGAG ACAAGCTGGG AGAAAGAGAC 
GACACCATCC CCCCTGAATA TAGACACCTC TTGGATAACG ATGAGGGTAA ACCAGGAAAG 
CCACCTGCCA AGAAAACCAA GGACTCAAAG AAGCCTGAAG ATGACAAAGA CCCCATCGAT 
GCCCTGTCAG GCGACTTCGA CAGCTGTCCT GCACCCACAG AAGCCTCAGA GAACACAGCA 
GAGGACAAAG CCAAGAGAAC TGCTCCCAGC TCCAAAACAC CGAAGAATAG GGGTAAAGCA 
AAGGATTCTG CAAAGGCAAA GCAGGAAACT TCCAAGCCCA AAGCTGATGA AAAAAATACA 
AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_848621.3
CDS	102..2507
Translation	MSQPGQKPAASPRPRRAAAARDTQEHVSEKTSGSPSKSGEKKGADEKKARSLESSQPSRTHAGGTAPATKASASSASTSKSSSMNPTETKAIPVSKQTGGQHSPNKKRHKKQAAKTEPEKKSQSTKPSVVHEKKTQEVKPKEHKEPKSLPKHASDMGSKHVHSEKAASRSSEQLTSEKSTKPKTKAQDSVSAGGESVVAGAAAAAASATAANKKKEKKSLTSAVPVESKPGKPPGKSDMDAALDDLVDTLGKPEETKEDNVPYTGPEISDPMTSTYIEELGKREVTLPPKYRELLAKKEGITVPPPDSSKPMGPNDAIDALSSDFTCSSPTDAGKKTKEEKSTGEEILKAQPARVTKSAASPQEKKRKVEEDAMSDQALEALSASLGSRKPEPELDLSSIKEVDEAKAKEEKLKKCGEDEETVPSEYRLKPATDKDGKPLLPEPEEKSKPLSESELIDELSEDFNQPKSKGEQSKPTEKTEESQAAPLTPVSEAVPRTSMCSIQSAPPKPPTLEGMVPDDAVEALAGSLGKKEADPGDGKPVEDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKVPKESLLPMSEDFLLDALSKDFAGSSNTSPLEFEEANLPAVISEVVSQTPAPTTHSAGLPPDTVQSDNKELDDALDQLSDSLGQRQPDPDENKPIEDKVKERAKAEHRDKLGERDDTIPPEYRHLLDNDEGKPGKPPAKKTKDSKKPEDDKDPIDALSGDFDSCPAPTEASENTAEDKAKRTAPSSKTPKNRGKAKDSAKAKQETSKPKADEKNTS

Target ORF information:

RefSeq Version	XM_843528.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calpastatin (CAST), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_843528.4

ORF Insert Sequence:

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTATTCCAG TCAGCAAACA GACGGGAGGA 
CAGCATTCTC CTAACAAGAA AAGACACAAA AAACAGGCTG CAAAAACAGA ACCTGAAAAG 
AAGTCACAAT CAACTAAGCC ATCTGTGGTC CATGAAAAAA AAACCCAAGA AGTAAAGCCA 
AAGGAACACA AAGAGCCAAA AAGCCTACCC AAGCATGCAT CAGATATGGG AAGCAAGCAT 
GTTCATAGTG AAAAAGCAGC TTCTAGATCA AGTGAGCAGC TGACATCAGA GAAATCAACA 
AAACCAAAGA CTAAAGCACA GGATTCCGTT TCTGCCGGTG GGGAGAGTGT TGTTGCAGGT 
GCAGCTGCAG CTGCAGCATC TGCCACAGCA GCTAACAAGA AAAAAGAAAA GAAATCACTA 
ACATCGGCTG TACCAGTTGA ATCCAAACCG GGTAAACCAC CTGGAAAGTC AGACATGGAT 
GCTGCTTTGG ATGACTTAGT AGACACTCTA GGAAAACCTG AAGAAACTAA AGAAGATAAT 
GTACCATATA CTGGACCTGA AATTTCAGAT CCGATGACTT CCACCTACAT AGAGGAATTA 
GGTAAAAGAG AAGTCACACT TCCTCCAAAG TACAGGGAAC TTTTGGCAAA AAAAGAAGGT 
ATCACAGTGC CTCCTCCAGA CTCTTCGAAA CCCATGGGGC CCAATGATGC CATAGATGCT 
TTGTCATCTG ACTTCACCTG CAGTTCACCT ACCGATGCTG GAAAGAAAAC TAAGGAAGAG 
AAATCTACAG GAGAAGAGAT TTTAAAAGCC CAGCCAGCCA GGGTAACCAA AAGTGCTGCT 
TCACCCCAAG AGAAAAAAAG AAAGGTGGAG GAGGACGCAA TGAGTGATCA AGCACTGGAG 
GCTCTTTCAG CTTCTTTGGG CAGCCGGAAG CCAGAACCCG AGCTGGACCT CAGTTCCATT 
AAGGAAGTCG ACGAGGCAAA AGCCAAAGAA GAGAAACTAA AGAAGTGTGG TGAAGATGAG 
GAAACTGTCC CATCAGAGTA CAGATTAAAA CCAGCTACGG ATAAAGATGG AAAACCTCTA 
TTGCCAGAGC CTGAAGAAAA ATCCAAGCCC CTGAGTGAAT CAGAACTCAT TGATGAGCTT 
TCAGAAGATT TTAACCAGCC TAAAAGTAAA GGAGAACAGT CTAAGCCAAC TGAAAAAACA 
GAAGAATCTC AGGCTGCCCC CCTAACTCCT GTGAGCGAGG CTGTGCCTCG GACCTCCATG 
TGTAGCATAC AGTCGGCACC GCCCAAGCCA CCTACCTTGG AAGGCATGGT GCCAGATGAT 
GCTGTAGAAG CCTTGGCTGG TAGCCTGGGG AAGAAGGAAG CAGATCCAGG AGATGGAAAG 
CCTGTGGAAG ATAAAGTCAA GGAGAAAGCC AAGGAAGAGG ATCGTGAAAA ACTTGGTGAA 
AAAGAAGAAA CAATTCCTCC TGATTATAGA TTAGAAGAGG TCAAGGATAA AGATGGAAAA 
CCACTCCTGC CAAAAGTGCC CAAGGAATCG CTTTTACCCA TGAGTGAAGA CTTCCTTCTT 
GATGCTTTGT CCAAGGACTT TGCTGGTTCC TCAAACACTT CACCTCTTGA ATTTGAAGAG 
GCTAACCTTC CTGCTGTCAT CTCTGAAGTG GTTTCCCAAA CCCCAGCTCC AACCACGCAC 
TCTGCTGGTC TACCCCCTGA TACTGTGCAA AGTGACAACA AAGAACTGGA CGATGCTTTG 
GACCAACTTT CTGACAGTCT GGGACAAAGG CAGCCTGATC CAGATGAGAA CAAGCCGATA 
GAGGATAAAG TCAAGGAAAG AGCTAAAGCT GAACACAGAG ACAAGCTGGG AGAAAGAGAC 
GACACCATCC CCCCTGAATA TAGACACCTC TTGGATAACG ATGAGGGTAA ACCAGGAAAG 
CCACCTGCCA AGAAAACCAA GGACTCAAAG AAGCCTGAAG ATGACAAAGA CCCCATCGAT 
GCCCTGTCAG GCGACTTCGA CAGCTGTCCT GCACCCACAG AAGCCTCAGA GAACACAGCA 
GAGGACAAAG CCAAGAGAAC TGCTCCCAGC TCCAAAACAC CGAAGAATAG GGGTAAAGCA 
AAGGATTCTG CAAAGGCAAA GCAGGAAACT TCCAAGCCCA AAGCTGATGA AAAAAATACA 
AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_022416703.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022272411.1 Canis lupus familiaris calpastatin (CAST), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OCe287108
Clone ID Related Accession (Same CDS sequence)	XM_022416703.1
Accession Version	XM_022416703.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	calpastatin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTATTCCAG TCAGCAAACA GACGGGAGGA 
CAGCATTCTC CTAACAAGAA AAGACACAAA AAACAGGCTG CAAAAACAGA ACCTGAAAAG 
AAGTCACAAT CAACTAAGCC AAAAAGCCTA CCCAAGCATG CATCAGATAT GGGAAGCAAG 
CATGTTCATA GTGAAAAAGC AGCTTCTAGA TCAAGTGAGC AGCTGACATC AGAGAAATCA 
ACAAAACCAA AGACTAAAGC ACAGGATTCC GTTTCTGCCG GTGGGGAGAG TGTTGTTGCA 
GGTGCAGCTG CAGCTGCAGC ATCTGCCACA GCAGCTAACA AGAAAAAAGA AAAGAAATCA 
CTAACATCGG CTGTACCAGT TGAATCCAAA CCGGGTAAAC CACCTGGAAA GTCAGACATG 
GATGCTGCTT TGGATGACTT AGTAGACACT CTAGGAAAAC CTGAAGAAAC TAAAGAAGAT 
AATGTACCAT ATACTGGACC TGAAATTTCA GATCCGATGA CTTCCACCTA CATAGAGGAA 
TTAGGTAAAA GAGAAGTCAC ACTTCCTCCA AAGTACAGGG AACTTTTGGC AAAAAAAGAA 
GGTATCACAG TGCCTCCTCC AGACTCTTCG AAACCCATGG GGCCCAATGA TGCCATAGAT 
GCTTTGTCAT CTGACTTCAC CTGCAGTTCA CCTACCGATG CTGGAAAGAA AACTAAGGAA 
GAGAAATCTA CAGGAGAAGA GATTTTAAAA GCCCAGCCAG CCAGGGTAAC CAAAAGTGCT 
GCTTCACCCC AAGAGAAAAA AAGAAAGGTG GAGGAGCAGG ACGCAATGAG TGATCAAGCA 
CTGGAGGCTC TTTCAGCTTC TTTGGGCAGC CGGAAGCCAG AACCCGAGCT GGACCTCAGT 
TCCATTAAGG AAGTCGACGA GGCAAAAGCC AAAGAAGAGA AACTAAAGAA GTGTGGTGAA 
GATGAGGAAA CTGTCCCATC AGAGTACAGA TTAAAACCAG CTACGGATAA AGATGGAAAA 
CCTCTATTGC CAGAGCCTGA AGAAAAATCC AAGCCCCTGA GTGAATCAGA ACTCATTGAT 
GAGCTTTCAG AAGATTTTAA CCAGCCTAAA AGTAAAGGAG AACAGTCTAA GCCAACTGAA 
AAAACAGAAG AATCTCAGGC TGCCCCCCTA ACTCCTGTGA GCGAGGCTGT GCCTCGGACC 
TCCATGTGTA GCATACAGTC GGCACCGCCC AAGCCACCTA CCTTGGAAGG CATGGTGCCA 
GATGATGCTG TAGAAGCCTT GGCTGGTAGC CTGGGGAAGA AGGAAGCAGA TCCAGGAGAT 
GGAAAGCCTG TGGAAGATAA AGTCAAGGAG AAAGCCAAGG AAGAGGATCG TGAAAAACTT 
GGTGAAAAAG AAGAAACAAT TCCTCCTGAT TATAGATTAG AAGAGGTCAA GGATAAAGAT 
GGAAAACCAC TCCTGCCAAA AGTGCCCAAG GAATCGCTTT TACCCATGAG TGAAGACTTC 
CTTCTTGATG CTTTGTCCAA GGACTTTGCT GGTTCCTCAA ACACTTCACC TCTTGAATTT 
GAAGAGGCTA ACCTTCCTGC TGTCATCTCT GAAGTGGTTT CCCAAACCCC AGCTCCAACC 
ACGCACTCTG CTGGTCTACC CCCTGATACT GTGCAAAGTG ACAACAAAGA ACTGGACGAT 
GCTTTGGACC AACTTTCTGA CAGTCTGGGA CAAAGGCAGC CTGATCCAGA TGAGAACAAG 
CCGATAGAGG ATAAAGTCAA GGAAAGAGCT AAAGCTGAAC ACAGAGACAA GCTGGGAGAA 
AGAGACGACA CCATCCCCCC TGAATATAGA CACCTCTTGG ATAACGATGA GGGTAAACCA 
GGAAAGCCAC CTGCCAAGAA AACCAAGGAC TCAAAGAAGC CTGAAGATGA CAAAGACCCC 
ATCGATGCCC TGTCAGGCGA CTTCGACAGC TGTCCTGCAC CCACAGAAGC CTCAGAGAAC 
ACAGCAGAGG ACAAAGCCAA GAGAACTGCT CCCAGCTCCA AAACACCGAA GAATAGGGGT 
AAAGCAAAGG ATTCTGCAAA GGCAAAGCAG GAAACTTCCA AGCCCAAAGC TGATGAAAAA 
AATACAAGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022272411.1
CDS	79..2430
Translation	MSQPGQKPAASPRPRRAAAARDTQEHVSEKTSGSPSKSGEKKGADEKKARSLESSQPSRTHAGGTAPATKASASSASTSKSSSMNPTETKAIPVSKQTGGQHSPNKKRHKKQAAKTEPEKKSQSTKPKSLPKHASDMGSKHVHSEKAASRSSEQLTSEKSTKPKTKAQDSVSAGGESVVAGAAAAAASATAANKKKEKKSLTSAVPVESKPGKPPGKSDMDAALDDLVDTLGKPEETKEDNVPYTGPEISDPMTSTYIEELGKREVTLPPKYRELLAKKEGITVPPPDSSKPMGPNDAIDALSSDFTCSSPTDAGKKTKEEKSTGEEILKAQPARVTKSAASPQEKKRKVEEQDAMSDQALEALSASLGSRKPEPELDLSSIKEVDEAKAKEEKLKKCGEDEETVPSEYRLKPATDKDGKPLLPEPEEKSKPLSESELIDELSEDFNQPKSKGEQSKPTEKTEESQAAPLTPVSEAVPRTSMCSIQSAPPKPPTLEGMVPDDAVEALAGSLGKKEADPGDGKPVEDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKVPKESLLPMSEDFLLDALSKDFAGSSNTSPLEFEEANLPAVISEVVSQTPAPTTHSAGLPPDTVQSDNKELDDALDQLSDSLGQRQPDPDENKPIEDKVKERAKAEHRDKLGERDDTIPPEYRHLLDNDEGKPGKPPAKKTKDSKKPEDDKDPIDALSGDFDSCPAPTEASENTAEDKAKRTAPSSKTPKNRGKAKDSAKAKQETSKPKADEKNTS

Target ORF information:

RefSeq Version	XM_022416703.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calpastatin (CAST), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022416703.1

ORF Insert Sequence:

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTATTCCAG TCAGCAAACA GACGGGAGGA 
CAGCATTCTC CTAACAAGAA AAGACACAAA AAACAGGCTG CAAAAACAGA ACCTGAAAAG 
AAGTCACAAT CAACTAAGCC AAAAAGCCTA CCCAAGCATG CATCAGATAT GGGAAGCAAG 
CATGTTCATA GTGAAAAAGC AGCTTCTAGA TCAAGTGAGC AGCTGACATC AGAGAAATCA 
ACAAAACCAA AGACTAAAGC ACAGGATTCC GTTTCTGCCG GTGGGGAGAG TGTTGTTGCA 
GGTGCAGCTG CAGCTGCAGC ATCTGCCACA GCAGCTAACA AGAAAAAAGA AAAGAAATCA 
CTAACATCGG CTGTACCAGT TGAATCCAAA CCGGGTAAAC CACCTGGAAA GTCAGACATG 
GATGCTGCTT TGGATGACTT AGTAGACACT CTAGGAAAAC CTGAAGAAAC TAAAGAAGAT 
AATGTACCAT ATACTGGACC TGAAATTTCA GATCCGATGA CTTCCACCTA CATAGAGGAA 
TTAGGTAAAA GAGAAGTCAC ACTTCCTCCA AAGTACAGGG AACTTTTGGC AAAAAAAGAA 
GGTATCACAG TGCCTCCTCC AGACTCTTCG AAACCCATGG GGCCCAATGA TGCCATAGAT 
GCTTTGTCAT CTGACTTCAC CTGCAGTTCA CCTACCGATG CTGGAAAGAA AACTAAGGAA 
GAGAAATCTA CAGGAGAAGA GATTTTAAAA GCCCAGCCAG CCAGGGTAAC CAAAAGTGCT 
GCTTCACCCC AAGAGAAAAA AAGAAAGGTG GAGGAGCAGG ACGCAATGAG TGATCAAGCA 
CTGGAGGCTC TTTCAGCTTC TTTGGGCAGC CGGAAGCCAG AACCCGAGCT GGACCTCAGT 
TCCATTAAGG AAGTCGACGA GGCAAAAGCC AAAGAAGAGA AACTAAAGAA GTGTGGTGAA 
GATGAGGAAA CTGTCCCATC AGAGTACAGA TTAAAACCAG CTACGGATAA AGATGGAAAA 
CCTCTATTGC CAGAGCCTGA AGAAAAATCC AAGCCCCTGA GTGAATCAGA ACTCATTGAT 
GAGCTTTCAG AAGATTTTAA CCAGCCTAAA AGTAAAGGAG AACAGTCTAA GCCAACTGAA 
AAAACAGAAG AATCTCAGGC TGCCCCCCTA ACTCCTGTGA GCGAGGCTGT GCCTCGGACC 
TCCATGTGTA GCATACAGTC GGCACCGCCC AAGCCACCTA CCTTGGAAGG CATGGTGCCA 
GATGATGCTG TAGAAGCCTT GGCTGGTAGC CTGGGGAAGA AGGAAGCAGA TCCAGGAGAT 
GGAAAGCCTG TGGAAGATAA AGTCAAGGAG AAAGCCAAGG AAGAGGATCG TGAAAAACTT 
GGTGAAAAAG AAGAAACAAT TCCTCCTGAT TATAGATTAG AAGAGGTCAA GGATAAAGAT 
GGAAAACCAC TCCTGCCAAA AGTGCCCAAG GAATCGCTTT TACCCATGAG TGAAGACTTC 
CTTCTTGATG CTTTGTCCAA GGACTTTGCT GGTTCCTCAA ACACTTCACC TCTTGAATTT 
GAAGAGGCTA ACCTTCCTGC TGTCATCTCT GAAGTGGTTT CCCAAACCCC AGCTCCAACC 
ACGCACTCTG CTGGTCTACC CCCTGATACT GTGCAAAGTG ACAACAAAGA ACTGGACGAT 
GCTTTGGACC AACTTTCTGA CAGTCTGGGA CAAAGGCAGC CTGATCCAGA TGAGAACAAG 
CCGATAGAGG ATAAAGTCAA GGAAAGAGCT AAAGCTGAAC ACAGAGACAA GCTGGGAGAA 
AGAGACGACA CCATCCCCCC TGAATATAGA CACCTCTTGG ATAACGATGA GGGTAAACCA 
GGAAAGCCAC CTGCCAAGAA AACCAAGGAC TCAAAGAAGC CTGAAGATGA CAAAGACCCC 
ATCGATGCCC TGTCAGGCGA CTTCGACAGC TGTCCTGCAC CCACAGAAGC CTCAGAGAAC 
ACAGCAGAGG ACAAAGCCAA GAGAACTGCT CCCAGCTCCA AAACACCGAA GAATAGGGGT 
AAAGCAAAGG ATTCTGCAAA GGCAAAGCAG GAAACTTCCA AGCCCAAAGC TGATGAAAAA 
AATACAAGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_022416704.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022272412.1 Canis lupus familiaris calpastatin (CAST), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OCe287109
Clone ID Related Accession (Same CDS sequence)	XM_022416704.1
Accession Version	XM_022416704.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	calpastatin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTATTCCAG TCAGCAAACA GACGGGAGGA 
CAGCATTCTC CTAACAAGAA AAGACACAAA AAACAGGCTG CAAAAACAGA ACCTGAAAAG 
AAGTCACAAT CAACTAAGCC AAAAAGCCTA CCCAAGCATG CATCAGATAT GGGAAGCAAG 
CATGTTCATA GTGAAAAAGC AGCTTCTAGA TCAAGTGAGC AGCTGACATC AGAGAAATCA 
ACAAAACCAA AGACTAAAGC ACAGGATTCC GTTTCTGCCG GTGGGGAGAG TGTTGTTGCA 
GGTGCAGCTG CAGCTGCAGC ATCTGCCACA GCAGCTAACA AGAAAAAAGA AAAGAAATCA 
CTAACATCGG CTGTACCAGT TGAATCCAAA CCGGGTAAAC CACCTGGAAA GTCAGACATG 
GATGCTGCTT TGGATGACTT AGTAGACACT CTAGGAAAAC CTGAAGAAAC TAAAGAAGAT 
AATGTACCAT ATACTGGACC TGAAATTTCA GATCCGATGA CTTCCACCTA CATAGAGGAA 
TTAGGTAAAA GAGAAGTCAC ACTTCCTCCA AAGTACAGGG AACTTTTGGC AAAAAAAGAA 
GGTATCACAG TGCCTCCTCC AGACTCTTCG AAACCCATGG GGCCCAATGA TGCCATAGAT 
GCTTTGTCAT CTGACTTCAC CTGCAGTTCA CCTACCGATG CTGGAAAGAA AACTAAGGAA 
GAGAAATCTA CAGGAGAAGA GATTTTAAAA GCCCAGCCAG CCAGGGTAAC CAAAAGTGCT 
GCTTCACCCC AAGAGAAAAA AAGAAAGGTG GAGGAGGACG CAATGAGTGA TCAAGCACTG 
GAGGCTCTTT CAGCTTCTTT GGGCAGCCGG AAGCCAGAAC CCGAGCTGGA CCTCAGTTCC 
ATTAAGGAAG TCGACGAGGC AAAAGCCAAA GAAGAGAAAC TAAAGAAGTG TGGTGAAGAT 
GAGGAAACTG TCCCATCAGA GTACAGATTA AAACCAGCTA CGGATAAAGA TGGAAAACCT 
CTATTGCCAG AGCCTGAAGA AAAATCCAAG CCCCTGAGTG AATCAGAACT CATTGATGAG 
CTTTCAGAAG ATTTTAACCA GCCTAAAAGT AAAGGAGAAC AGTCTAAGCC AACTGAAAAA 
ACAGAAGAAT CTCAGGCTGC CCCCCTAACT CCTGTGAGCG AGGCTGTGCC TCGGACCTCC 
ATGTGTAGCA TACAGTCGGC ACCGCCCAAG CCACCTACCT TGGAAGGCAT GGTGCCAGAT 
GATGCTGTAG AAGCCTTGGC TGGTAGCCTG GGGAAGAAGG AAGCAGATCC AGGAGATGGA 
AAGCCTGTGG AAGATAAAGT CAAGGAGAAA GCCAAGGAAG AGGATCGTGA AAAACTTGGT 
GAAAAAGAAG AAACAATTCC TCCTGATTAT AGATTAGAAG AGGTCAAGGA TAAAGATGGA 
AAACCACTCC TGCCAAAAGT GCCCAAGGAA TCGCTTTTAC CCATGAGTGA AGACTTCCTT 
CTTGATGCTT TGTCCAAGGA CTTTGCTGGT TCCTCAAACA CTTCACCTCT TGAATTTGAA 
GAGGCTAACC TTCCTGCTGT CATCTCTGAA GTGGTTTCCC AAACCCCAGC TCCAACCACG 
CACTCTGCTG GTCTACCCCC TGATACTGTG CAAAGTGACA ACAAAGAACT GGACGATGCT 
TTGGACCAAC TTTCTGACAG TCTGGGACAA AGGCAGCCTG ATCCAGATGA GAACAAGCCG 
ATAGAGGATA AAGTCAAGGA AAGAGCTAAA GCTGAACACA GAGACAAGCT GGGAGAAAGA 
GACGACACCA TCCCCCCTGA ATATAGACAC CTCTTGGATA ACGATGAGGG TAAACCAGGA 
AAGCCACCTG CCAAGAAAAC CAAGGACTCA AAGAAGCCTG AAGATGACAA AGACCCCATC 
GATGCCCTGT CAGGCGACTT CGACAGCTGT CCTGCACCCA CAGAAGCCTC AGAGAACACA 
GCAGAGGACA AAGCCAAGAG AACTGCTCCC AGCTCCAAAA CACCGAAGAA TAGGGGTAAA 
GCAAAGGATT CTGCAAAGGC AAAGCAGGAA ACTTCCAAGC CCAAAGCTGA TGAAAAAAAT 
ACAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022272412.1
CDS	79..2427
Translation	MSQPGQKPAASPRPRRAAAARDTQEHVSEKTSGSPSKSGEKKGADEKKARSLESSQPSRTHAGGTAPATKASASSASTSKSSSMNPTETKAIPVSKQTGGQHSPNKKRHKKQAAKTEPEKKSQSTKPKSLPKHASDMGSKHVHSEKAASRSSEQLTSEKSTKPKTKAQDSVSAGGESVVAGAAAAAASATAANKKKEKKSLTSAVPVESKPGKPPGKSDMDAALDDLVDTLGKPEETKEDNVPYTGPEISDPMTSTYIEELGKREVTLPPKYRELLAKKEGITVPPPDSSKPMGPNDAIDALSSDFTCSSPTDAGKKTKEEKSTGEEILKAQPARVTKSAASPQEKKRKVEEDAMSDQALEALSASLGSRKPEPELDLSSIKEVDEAKAKEEKLKKCGEDEETVPSEYRLKPATDKDGKPLLPEPEEKSKPLSESELIDELSEDFNQPKSKGEQSKPTEKTEESQAAPLTPVSEAVPRTSMCSIQSAPPKPPTLEGMVPDDAVEALAGSLGKKEADPGDGKPVEDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKVPKESLLPMSEDFLLDALSKDFAGSSNTSPLEFEEANLPAVISEVVSQTPAPTTHSAGLPPDTVQSDNKELDDALDQLSDSLGQRQPDPDENKPIEDKVKERAKAEHRDKLGERDDTIPPEYRHLLDNDEGKPGKPPAKKTKDSKKPEDDKDPIDALSGDFDSCPAPTEASENTAEDKAKRTAPSSKTPKNRGKAKDSAKAKQETSKPKADEKNTS

Target ORF information:

RefSeq Version	XM_022416704.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calpastatin (CAST), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022416704.1

ORF Insert Sequence:

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTATTCCAG TCAGCAAACA GACGGGAGGA 
CAGCATTCTC CTAACAAGAA AAGACACAAA AAACAGGCTG CAAAAACAGA ACCTGAAAAG 
AAGTCACAAT CAACTAAGCC AAAAAGCCTA CCCAAGCATG CATCAGATAT GGGAAGCAAG 
CATGTTCATA GTGAAAAAGC AGCTTCTAGA TCAAGTGAGC AGCTGACATC AGAGAAATCA 
ACAAAACCAA AGACTAAAGC ACAGGATTCC GTTTCTGCCG GTGGGGAGAG TGTTGTTGCA 
GGTGCAGCTG CAGCTGCAGC ATCTGCCACA GCAGCTAACA AGAAAAAAGA AAAGAAATCA 
CTAACATCGG CTGTACCAGT TGAATCCAAA CCGGGTAAAC CACCTGGAAA GTCAGACATG 
GATGCTGCTT TGGATGACTT AGTAGACACT CTAGGAAAAC CTGAAGAAAC TAAAGAAGAT 
AATGTACCAT ATACTGGACC TGAAATTTCA GATCCGATGA CTTCCACCTA CATAGAGGAA 
TTAGGTAAAA GAGAAGTCAC ACTTCCTCCA AAGTACAGGG AACTTTTGGC AAAAAAAGAA 
GGTATCACAG TGCCTCCTCC AGACTCTTCG AAACCCATGG GGCCCAATGA TGCCATAGAT 
GCTTTGTCAT CTGACTTCAC CTGCAGTTCA CCTACCGATG CTGGAAAGAA AACTAAGGAA 
GAGAAATCTA CAGGAGAAGA GATTTTAAAA GCCCAGCCAG CCAGGGTAAC CAAAAGTGCT 
GCTTCACCCC AAGAGAAAAA AAGAAAGGTG GAGGAGGACG CAATGAGTGA TCAAGCACTG 
GAGGCTCTTT CAGCTTCTTT GGGCAGCCGG AAGCCAGAAC CCGAGCTGGA CCTCAGTTCC 
ATTAAGGAAG TCGACGAGGC AAAAGCCAAA GAAGAGAAAC TAAAGAAGTG TGGTGAAGAT 
GAGGAAACTG TCCCATCAGA GTACAGATTA AAACCAGCTA CGGATAAAGA TGGAAAACCT 
CTATTGCCAG AGCCTGAAGA AAAATCCAAG CCCCTGAGTG AATCAGAACT CATTGATGAG 
CTTTCAGAAG ATTTTAACCA GCCTAAAAGT AAAGGAGAAC AGTCTAAGCC AACTGAAAAA 
ACAGAAGAAT CTCAGGCTGC CCCCCTAACT CCTGTGAGCG AGGCTGTGCC TCGGACCTCC 
ATGTGTAGCA TACAGTCGGC ACCGCCCAAG CCACCTACCT TGGAAGGCAT GGTGCCAGAT 
GATGCTGTAG AAGCCTTGGC TGGTAGCCTG GGGAAGAAGG AAGCAGATCC AGGAGATGGA 
AAGCCTGTGG AAGATAAAGT CAAGGAGAAA GCCAAGGAAG AGGATCGTGA AAAACTTGGT 
GAAAAAGAAG AAACAATTCC TCCTGATTAT AGATTAGAAG AGGTCAAGGA TAAAGATGGA 
AAACCACTCC TGCCAAAAGT GCCCAAGGAA TCGCTTTTAC CCATGAGTGA AGACTTCCTT 
CTTGATGCTT TGTCCAAGGA CTTTGCTGGT TCCTCAAACA CTTCACCTCT TGAATTTGAA 
GAGGCTAACC TTCCTGCTGT CATCTCTGAA GTGGTTTCCC AAACCCCAGC TCCAACCACG 
CACTCTGCTG GTCTACCCCC TGATACTGTG CAAAGTGACA ACAAAGAACT GGACGATGCT 
TTGGACCAAC TTTCTGACAG TCTGGGACAA AGGCAGCCTG ATCCAGATGA GAACAAGCCG 
ATAGAGGATA AAGTCAAGGA AAGAGCTAAA GCTGAACACA GAGACAAGCT GGGAGAAAGA 
GACGACACCA TCCCCCCTGA ATATAGACAC CTCTTGGATA ACGATGAGGG TAAACCAGGA 
AAGCCACCTG CCAAGAAAAC CAAGGACTCA AAGAAGCCTG AAGATGACAA AGACCCCATC 
GATGCCCTGT CAGGCGACTT CGACAGCTGT CCTGCACCCA CAGAAGCCTC AGAGAACACA 
GCAGAGGACA AAGCCAAGAG AACTGCTCCC AGCTCCAAAA CACCGAAGAA TAGGGGTAAA 
GCAAAGGATT CTGCAAAGGC AAAGCAGGAA ACTTCCAAGC CCAAAGCTGA TGAAAAAAAT 
ACAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_022416705.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022272413.1 Canis lupus familiaris calpastatin (CAST), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OCe287110
Clone ID Related Accession (Same CDS sequence)	XM_022416705.1
Accession Version	XM_022416705.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	calpastatin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTATTCCAG TCAGCAAACA GACGGGAGGA 
CAGCATTCTC CTAACAAGAA AAGACACAAA AAACAGGCTG CAAAAACAGA ACCTGAAAAG 
AAGTCACAAT CAACTAAGCC ATCTGTGGTC CATGAAAAAA AAACCCAAGA AGTAAAGCCA 
AAGGAACACA AAGAGCCAAA AAGCCTACCC AAGCATGCAT CAGATATGGG AAGCAAGCAT 
GTTCATAGTG AAAAAGCAGC TTCTAGATCA AGTGAGCAGC TGACATCAGA GAAATCAACA 
AAACCAAAGA CTAAAGCACA GGATTCCGTT TCTGCCGGTG GGGAGAGTGT TGTTGCAGGT 
GCAGCTGCAG CTGCAGCATC TGCCACAGCA GCTAACAAGC AGAAAAAAGA AAAGAAATCA 
CTAACATCGG CTGTACCAGT TGAATCCAAA CCGGGTAAAC CACCTGGAAA GTCAGACATG 
GATGCTGCTT TGGATGACTT AGTAGACACT CTAGGAAAAC CTGAAGAAAC TAAAGAAGAT 
AATGTACCAT ATACTGGACC TGAAATTTCA GATCCGATGA CTTCCACCTA CATAGAGGAA 
TTAGGTAAAA GAGAAGTCAC ACTTCCTCCA AAGTACAGGG AACTTTTGGC AAAAAAAGAA 
GGTATCACAG TGCCTCCTCC AGACTCTTCG AAACCCATGG GGCCCAATGA TGCCATAGAT 
GCTTTGTCAT CTGACTTCAC CTGCAGTTCA CCTACCGATG CTGGAAAGAA AACTAAGGAA 
GAGAAATCTA CAGGAGAAGA GATTTTAAAA GCCCAGCCAG CCAGGGTAAC CAAAAGTGCT 
GCTTCACCCC AAGAGAAAAA AAGAAAGGTG GAGGAGCAGG ACGCAATGAG TGATCAAGCA 
CTGGAGGCTC TTTCAGCTTC TTTGGGCAGC CGGAAGCCAG AACCCGAGCT GGACCTCAGT 
TCCATTAAGG AAGTCGACGA GGCAAAAGCC AAAGAAGAGA AACTAAAGAA GTGTGGTGAA 
GATGAGGAAA CTGTCCCATC AGAGTACAGA TTAAAACCAG CTACGGATAA AGATGGAAAA 
CCTCTATTGC CAGAGCCTGA AGAAAAATCC AAGCCCCTGA GTGAATCAGA ACTCATTGAT 
GAGCTTTCAG AAGATTTTAA CCAGCCTAAA AGTAAAGGAG AACAGTCTAA GCCAACTGAA 
AAAACAGAAG AAGGCATGGT GCCAGATGAT GCTGTAGAAG CCTTGGCTGG TAGCCTGGGG 
AAGAAGGAAG CAGATCCAGG AGATGGAAAG CCTGTGGAAG ATAAAGTCAA GGAGAAAGCC 
AAGGAAGAGG ATCGTGAAAA ACTTGGTGAA AAAGAAGAAA CAATTCCTCC TGATTATAGA 
TTAGAAGAGG TCAAGGATAA AGATGGAAAA CCACTCCTGC CAAAAGTGCC CAAGGAATCG 
CTTTTACCCA TGAGTGAAGA CTTCCTTCTT GATGCTTTGT CCAAGGACTT TGCTGGTTCC 
TCAAACACTT CACCTCTTGA ATTTGAAGAG GCTAACCTTC CTGCTGTCAT CTCTGAAGTG 
GTTTCCCAAA CCCCAGCTCC AACCACGCAC TCTGCTGGTC TACCCCCTGA TACTGTGCAA 
AGTGACAACA AAGAACTGGA CGATGCTTTG GACCAACTTT CTGACAGTCT GGGACAAAGG 
CAGCCTGATC CAGATGAGAA CAAGCCGATA GAGGATAAAG TCAAGGAAAG AGCTAAAGCT 
GAACACAGAG ACAAGCTGGG AGAAAGAGAC GACACCATCC CCCCTGAATA TAGACACCTC 
TTGGATAACG ATGAGGGTAA ACCAGGAAAG CCACCTGCCA AGAAAACCAA GGACTCAAAG 
AAGCCTGAAG ATGACAAAGA CCCCATCGAT GCCCTGTCAG GCGACTTCGA CAGCTGTCCT 
GCACCCACAG AAGCCTCAGA GAACACAGCA GAGGACAAAG CCAAGAGAAC TGCTCCCAGC 
TCCAAAACAC CGAAGAATAG GGGTAAAGCA AAGGATTCTG CAAAGGCAAA GCAGGAAACT 
TCCAAGCCCA AAGCTGATGA AAAAAATACA AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022272413.1
CDS	80..2395
Translation	MSQPGQKPAASPRPRRAAAARDTQEHVSEKTSGSPSKSGEKKGADEKKARSLESSQPSRTHAGGTAPATKASASSASTSKSSSMNPTETKAIPVSKQTGGQHSPNKKRHKKQAAKTEPEKKSQSTKPSVVHEKKTQEVKPKEHKEPKSLPKHASDMGSKHVHSEKAASRSSEQLTSEKSTKPKTKAQDSVSAGGESVVAGAAAAAASATAANKQKKEKKSLTSAVPVESKPGKPPGKSDMDAALDDLVDTLGKPEETKEDNVPYTGPEISDPMTSTYIEELGKREVTLPPKYRELLAKKEGITVPPPDSSKPMGPNDAIDALSSDFTCSSPTDAGKKTKEEKSTGEEILKAQPARVTKSAASPQEKKRKVEEQDAMSDQALEALSASLGSRKPEPELDLSSIKEVDEAKAKEEKLKKCGEDEETVPSEYRLKPATDKDGKPLLPEPEEKSKPLSESELIDELSEDFNQPKSKGEQSKPTEKTEEGMVPDDAVEALAGSLGKKEADPGDGKPVEDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKVPKESLLPMSEDFLLDALSKDFAGSSNTSPLEFEEANLPAVISEVVSQTPAPTTHSAGLPPDTVQSDNKELDDALDQLSDSLGQRQPDPDENKPIEDKVKERAKAEHRDKLGERDDTIPPEYRHLLDNDEGKPGKPPAKKTKDSKKPEDDKDPIDALSGDFDSCPAPTEASENTAEDKAKRTAPSSKTPKNRGKAKDSAKAKQETSKPKADEKNTS

Target ORF information:

RefSeq Version	XM_022416705.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calpastatin (CAST), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022416705.1

ORF Insert Sequence:

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTATTCCAG TCAGCAAACA GACGGGAGGA 
CAGCATTCTC CTAACAAGAA AAGACACAAA AAACAGGCTG CAAAAACAGA ACCTGAAAAG 
AAGTCACAAT CAACTAAGCC ATCTGTGGTC CATGAAAAAA AAACCCAAGA AGTAAAGCCA 
AAGGAACACA AAGAGCCAAA AAGCCTACCC AAGCATGCAT CAGATATGGG AAGCAAGCAT 
GTTCATAGTG AAAAAGCAGC TTCTAGATCA AGTGAGCAGC TGACATCAGA GAAATCAACA 
AAACCAAAGA CTAAAGCACA GGATTCCGTT TCTGCCGGTG GGGAGAGTGT TGTTGCAGGT 
GCAGCTGCAG CTGCAGCATC TGCCACAGCA GCTAACAAGC AGAAAAAAGA AAAGAAATCA 
CTAACATCGG CTGTACCAGT TGAATCCAAA CCGGGTAAAC CACCTGGAAA GTCAGACATG 
GATGCTGCTT TGGATGACTT AGTAGACACT CTAGGAAAAC CTGAAGAAAC TAAAGAAGAT 
AATGTACCAT ATACTGGACC TGAAATTTCA GATCCGATGA CTTCCACCTA CATAGAGGAA 
TTAGGTAAAA GAGAAGTCAC ACTTCCTCCA AAGTACAGGG AACTTTTGGC AAAAAAAGAA 
GGTATCACAG TGCCTCCTCC AGACTCTTCG AAACCCATGG GGCCCAATGA TGCCATAGAT 
GCTTTGTCAT CTGACTTCAC CTGCAGTTCA CCTACCGATG CTGGAAAGAA AACTAAGGAA 
GAGAAATCTA CAGGAGAAGA GATTTTAAAA GCCCAGCCAG CCAGGGTAAC CAAAAGTGCT 
GCTTCACCCC AAGAGAAAAA AAGAAAGGTG GAGGAGCAGG ACGCAATGAG TGATCAAGCA 
CTGGAGGCTC TTTCAGCTTC TTTGGGCAGC CGGAAGCCAG AACCCGAGCT GGACCTCAGT 
TCCATTAAGG AAGTCGACGA GGCAAAAGCC AAAGAAGAGA AACTAAAGAA GTGTGGTGAA 
GATGAGGAAA CTGTCCCATC AGAGTACAGA TTAAAACCAG CTACGGATAA AGATGGAAAA 
CCTCTATTGC CAGAGCCTGA AGAAAAATCC AAGCCCCTGA GTGAATCAGA ACTCATTGAT 
GAGCTTTCAG AAGATTTTAA CCAGCCTAAA AGTAAAGGAG AACAGTCTAA GCCAACTGAA 
AAAACAGAAG AAGGCATGGT GCCAGATGAT GCTGTAGAAG CCTTGGCTGG TAGCCTGGGG 
AAGAAGGAAG CAGATCCAGG AGATGGAAAG CCTGTGGAAG ATAAAGTCAA GGAGAAAGCC 
AAGGAAGAGG ATCGTGAAAA ACTTGGTGAA AAAGAAGAAA CAATTCCTCC TGATTATAGA 
TTAGAAGAGG TCAAGGATAA AGATGGAAAA CCACTCCTGC CAAAAGTGCC CAAGGAATCG 
CTTTTACCCA TGAGTGAAGA CTTCCTTCTT GATGCTTTGT CCAAGGACTT TGCTGGTTCC 
TCAAACACTT CACCTCTTGA ATTTGAAGAG GCTAACCTTC CTGCTGTCAT CTCTGAAGTG 
GTTTCCCAAA CCCCAGCTCC AACCACGCAC TCTGCTGGTC TACCCCCTGA TACTGTGCAA 
AGTGACAACA AAGAACTGGA CGATGCTTTG GACCAACTTT CTGACAGTCT GGGACAAAGG 
CAGCCTGATC CAGATGAGAA CAAGCCGATA GAGGATAAAG TCAAGGAAAG AGCTAAAGCT 
GAACACAGAG ACAAGCTGGG AGAAAGAGAC GACACCATCC CCCCTGAATA TAGACACCTC 
TTGGATAACG ATGAGGGTAA ACCAGGAAAG CCACCTGCCA AGAAAACCAA GGACTCAAAG 
AAGCCTGAAG ATGACAAAGA CCCCATCGAT GCCCTGTCAG GCGACTTCGA CAGCTGTCCT 
GCACCCACAG AAGCCTCAGA GAACACAGCA GAGGACAAAG CCAAGAGAAC TGCTCCCAGC 
TCCAAAACAC CGAAGAATAG GGGTAAAGCA AAGGATTCTG CAAAGGCAAA GCAGGAAACT 
TCCAAGCCCA AAGCTGATGA AAAAAATACA AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_005618074.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005618131.2 Canis lupus familiaris calpastatin (CAST), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe35581
Clone ID Related Accession (Same CDS sequence)	XM_005618074.3 , XM_005618074.2
Accession Version	XM_005618074.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	calpastatin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005618074.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTATTCCAG TCAGCAAACA GACGGGAGGA 
CAGCATTCTC CTAACAAGAA AAGACACAAA AAACAGGCTG CAAAAACAGA ACCTGAAAAG 
AAGTCACAAT CAACTAAGCC ATCTGTGGTC CATGAAAAAA AAACCCAAGA AGTAAAGCCA 
AAGGAACACA AAGAGCCAAA AAGCCTACCC AAGCATGCAT CAGATATGGG AAGCAAGCAT 
GTTCATAGTG AAAAAGCAGC TTCTAGATCA AGTGAGCAGC TGACATCAGA GAAATCAACA 
AAACCAAAGA CTAAAGCACA GGATTCCGTT TCTGCCGGTG GGGAGAGTGT TGTTGCAGGT 
GCAGCTGCAG CTGCAGCATC TGCCACAGCA GCTAACAAGA AAAAAGAAAA GAAATCACTA 
ACATCGGCTG TACCAGTTGA ATCCAAACCG GGTAAACCAC CTGGAAAGTC AGACATGGAT 
GCTGCTTTGG ATGACTTAGT AGACACTCTA GGAAAACCTG AAGAAACTAA AGAAGATAAT 
GTACCATATA CTGGACCTGA AATTTCAGAT CCGATGACTT CCACCTACAT AGAGGAATTA 
GGTAAAAGAG AAGTCACACT TCCTCCAAAG TACAGGGAAC TTTTGGCAAA AAAAGAAGGT 
ATCACAGTGC CTCCTCCAGA CTCTTCGAAA CCCATGGGGC CCAATGATGC CATAGATGCT 
TTGTCATCTG ACTTCACCTG CAGTTCACCT ACCGATGCTG GAAAGAAAAC TAAGGAAGAG 
AAATCTACAG GAGAAGAGAT TTTAAAAGCC CAGCCAGCCA GGGTAACCAA AAGTGCTGCT 
TCACCCCAAG AGAAAAAAAG AAAGGTGGAG GAGCAGGACG CAATGAGTGA TCAAGCACTG 
GAGGCTCTTT CAGCTTCTTT GGGCAGCCGG AAGCCAGAAC CCGAGCTGGA CCTCAGTTCC 
ATTAAGGAAG TCGACGAGGC AAAAGCCAAA GAAGAGAAAC TAAAGAAGTG TGGTGAAGAT 
GAGGAAACTG TCCCATCAGA GTACAGATTA AAACCAGCTA CGGATAAAGA TGGAAAACCT 
CTATTGCCAG AGCCTGAAGA AAAATCCAAG CCCCTGAGTG AATCAGAACT CATTGATGAG 
CTTTCAGAAG ATTTTAACCA GCCTAAAAGT AAAGGAGAAC AGTCTAAGCC AACTGAAAAA 
ACAGAAGAAT CTCAGGCTGC CCCCCTAACT CCTGTGAGCG AGGCTGTGCC TCGGACCTCC 
ATGTGTAGCA TACAGTCGGC ACCGCCCAAG CCACCTACCT TGGAAGGCAT GGTGCCAGAT 
GATGCTGTAG AAGCCTTGGC TGGTAGCCTG GGGAAGAAGG AAGCAGATCC AGGAGATGGA 
AAGCCTGTGG AAGATAAAGT CAAGGAGAAA GCCAAGGAAG AGGATCGTGA AAAACTTGGT 
GAAAAAGAAG AAACAATTCC TCCTGATTAT AGATTAGAAG AGGTCAAGGA TAAAGATGGA 
AAACCACTCC TGCCAAAAGT GCCCAAGGAA TCGCTTTTAC CCATGAGTGA AGACTTCCTT 
CTTGATGCTT TGTCCAAGGA CTTTGCTGGT TCCTCAAACA CTTCACCTCT TGAATTTGAA 
GAGGCTAACC TTCCTGCTGT CATCTCTGAA GTGGTTTCCC AAACCCCAGC TCCAACCACG 
CACTCTGCTG GTCTACCCCC TGATACTGTG CAAAGTGACA ACAAAGAACT GGACGATGCT 
TTGGACCAAC TTTCTGACAG TCTGGGACAA AGGCAGCCTG ATCCAGATGA GAACAAGCCG 
ATAGAGGATA AAGTCAAGGA AAGAGCTAAA GCTGAACACA GAGACAAGCT GGGAGAAAGA 
GACGACACCA TCCCCCCTGA ATATAGACAC CTCTTGGATA ACGATGAGGG TAAACCAGGA 
AAGCCACCTG CCAAGAAAAC CAAGGACTCA AAGAAGCCTG AAGATGACAA AGACCCCATC 
GATGCCCTGT CAGGCGACTT CGACAGCTGT CCTGCACCCA CAGAAGCCTC AGAGAACACA 
GCAGAGGACA AAGCCAAGAG AACTGCTCCC AGCTCCAAAA CACCGAAGAA TAGGGGTAAA 
GCAAAGGATT CTGCAAAGGC AAAGCAGGAA ACTTCCAAGC CCAAAGCTGA TGAAAAAAAT 
ACAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005618131.2
CDS	79..2487
Translation	MSQPGQKPAASPRPRRAAAARDTQEHVSEKTSGSPSKSGEKKGADEKKARSLESSQPSRTHAGGTAPATKASASSASTSKSSSMNPTETKAIPVSKQTGGQHSPNKKRHKKQAAKTEPEKKSQSTKPSVVHEKKTQEVKPKEHKEPKSLPKHASDMGSKHVHSEKAASRSSEQLTSEKSTKPKTKAQDSVSAGGESVVAGAAAAAASATAANKKKEKKSLTSAVPVESKPGKPPGKSDMDAALDDLVDTLGKPEETKEDNVPYTGPEISDPMTSTYIEELGKREVTLPPKYRELLAKKEGITVPPPDSSKPMGPNDAIDALSSDFTCSSPTDAGKKTKEEKSTGEEILKAQPARVTKSAASPQEKKRKVEEQDAMSDQALEALSASLGSRKPEPELDLSSIKEVDEAKAKEEKLKKCGEDEETVPSEYRLKPATDKDGKPLLPEPEEKSKPLSESELIDELSEDFNQPKSKGEQSKPTEKTEESQAAPLTPVSEAVPRTSMCSIQSAPPKPPTLEGMVPDDAVEALAGSLGKKEADPGDGKPVEDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKVPKESLLPMSEDFLLDALSKDFAGSSNTSPLEFEEANLPAVISEVVSQTPAPTTHSAGLPPDTVQSDNKELDDALDQLSDSLGQRQPDPDENKPIEDKVKERAKAEHRDKLGERDDTIPPEYRHLLDNDEGKPGKPPAKKTKDSKKPEDDKDPIDALSGDFDSCPAPTEASENTAEDKAKRTAPSSKTPKNRGKAKDSAKAKQETSKPKADEKNTS

Target ORF information:

RefSeq Version	XM_005618074.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calpastatin (CAST), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005618074.3

ORF Insert Sequence:

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTATTCCAG TCAGCAAACA GACGGGAGGA 
CAGCATTCTC CTAACAAGAA AAGACACAAA AAACAGGCTG CAAAAACAGA ACCTGAAAAG 
AAGTCACAAT CAACTAAGCC ATCTGTGGTC CATGAAAAAA AAACCCAAGA AGTAAAGCCA 
AAGGAACACA AAGAGCCAAA AAGCCTACCC AAGCATGCAT CAGATATGGG AAGCAAGCAT 
GTTCATAGTG AAAAAGCAGC TTCTAGATCA AGTGAGCAGC TGACATCAGA GAAATCAACA 
AAACCAAAGA CTAAAGCACA GGATTCCGTT TCTGCCGGTG GGGAGAGTGT TGTTGCAGGT 
GCAGCTGCAG CTGCAGCATC TGCCACAGCA GCTAACAAGA AAAAAGAAAA GAAATCACTA 
ACATCGGCTG TACCAGTTGA ATCCAAACCG GGTAAACCAC CTGGAAAGTC AGACATGGAT 
GCTGCTTTGG ATGACTTAGT AGACACTCTA GGAAAACCTG AAGAAACTAA AGAAGATAAT 
GTACCATATA CTGGACCTGA AATTTCAGAT CCGATGACTT CCACCTACAT AGAGGAATTA 
GGTAAAAGAG AAGTCACACT TCCTCCAAAG TACAGGGAAC TTTTGGCAAA AAAAGAAGGT 
ATCACAGTGC CTCCTCCAGA CTCTTCGAAA CCCATGGGGC CCAATGATGC CATAGATGCT 
TTGTCATCTG ACTTCACCTG CAGTTCACCT ACCGATGCTG GAAAGAAAAC TAAGGAAGAG 
AAATCTACAG GAGAAGAGAT TTTAAAAGCC CAGCCAGCCA GGGTAACCAA AAGTGCTGCT 
TCACCCCAAG AGAAAAAAAG AAAGGTGGAG GAGCAGGACG CAATGAGTGA TCAAGCACTG 
GAGGCTCTTT CAGCTTCTTT GGGCAGCCGG AAGCCAGAAC CCGAGCTGGA CCTCAGTTCC 
ATTAAGGAAG TCGACGAGGC AAAAGCCAAA GAAGAGAAAC TAAAGAAGTG TGGTGAAGAT 
GAGGAAACTG TCCCATCAGA GTACAGATTA AAACCAGCTA CGGATAAAGA TGGAAAACCT 
CTATTGCCAG AGCCTGAAGA AAAATCCAAG CCCCTGAGTG AATCAGAACT CATTGATGAG 
CTTTCAGAAG ATTTTAACCA GCCTAAAAGT AAAGGAGAAC AGTCTAAGCC AACTGAAAAA 
ACAGAAGAAT CTCAGGCTGC CCCCCTAACT CCTGTGAGCG AGGCTGTGCC TCGGACCTCC 
ATGTGTAGCA TACAGTCGGC ACCGCCCAAG CCACCTACCT TGGAAGGCAT GGTGCCAGAT 
GATGCTGTAG AAGCCTTGGC TGGTAGCCTG GGGAAGAAGG AAGCAGATCC AGGAGATGGA 
AAGCCTGTGG AAGATAAAGT CAAGGAGAAA GCCAAGGAAG AGGATCGTGA AAAACTTGGT 
GAAAAAGAAG AAACAATTCC TCCTGATTAT AGATTAGAAG AGGTCAAGGA TAAAGATGGA 
AAACCACTCC TGCCAAAAGT GCCCAAGGAA TCGCTTTTAC CCATGAGTGA AGACTTCCTT 
CTTGATGCTT TGTCCAAGGA CTTTGCTGGT TCCTCAAACA CTTCACCTCT TGAATTTGAA 
GAGGCTAACC TTCCTGCTGT CATCTCTGAA GTGGTTTCCC AAACCCCAGC TCCAACCACG 
CACTCTGCTG GTCTACCCCC TGATACTGTG CAAAGTGACA ACAAAGAACT GGACGATGCT 
TTGGACCAAC TTTCTGACAG TCTGGGACAA AGGCAGCCTG ATCCAGATGA GAACAAGCCG 
ATAGAGGATA AAGTCAAGGA AAGAGCTAAA GCTGAACACA GAGACAAGCT GGGAGAAAGA 
GACGACACCA TCCCCCCTGA ATATAGACAC CTCTTGGATA ACGATGAGGG TAAACCAGGA 
AAGCCACCTG CCAAGAAAAC CAAGGACTCA AAGAAGCCTG AAGATGACAA AGACCCCATC 
GATGCCCTGT CAGGCGACTT CGACAGCTGT CCTGCACCCA CAGAAGCCTC AGAGAACACA 
GCAGAGGACA AAGCCAAGAG AACTGCTCCC AGCTCCAAAA CACCGAAGAA TAGGGGTAAA 
GCAAAGGATT CTGCAAAGGC AAAGCAGGAA ACTTCCAAGC CCAAAGCTGA TGAAAAAAAT 
ACAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_005618074.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005618131.2 Canis lupus familiaris calpastatin (CAST), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe35581
Clone ID Related Accession (Same CDS sequence)	XM_005618074.3 , XM_005618074.2
Accession Version	XM_005618074.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	calpastatin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726052.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005618074.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTATTCCAG TCAGCAAACA GACGGGAGGA 
CAGCATTCTC CTAACAAGAA AAGACACAAA AAACAGGCTG CAAAAACAGA ACCTGAAAAG 
AAGTCACAAT CAACTAAGCC ATCTGTGGTC CATGAAAAAA AAACCCAAGA AGTAAAGCCA 
AAGGAACACA AAGAGCCAAA AAGCCTACCC AAGCATGCAT CAGATATGGG AAGCAAGCAT 
GTTCATAGTG AAAAAGCAGC TTCTAGATCA AGTGAGCAGC TGACATCAGA GAAATCAACA 
AAACCAAAGA CTAAAGCACA GGATTCCGTT TCTGCCGGTG GGGAGAGTGT TGTTGCAGGT 
GCAGCTGCAG CTGCAGCATC TGCCACAGCA GCTAACAAGA AAAAAGAAAA GAAATCACTA 
ACATCGGCTG TACCAGTTGA ATCCAAACCG GGTAAACCAC CTGGAAAGTC AGACATGGAT 
GCTGCTTTGG ATGACTTAGT AGACACTCTA GGAAAACCTG AAGAAACTAA AGAAGATAAT 
GTACCATATA CTGGACCTGA AATTTCAGAT CCGATGACTT CCACCTACAT AGAGGAATTA 
GGTAAAAGAG AAGTCACACT TCCTCCAAAG TACAGGGAAC TTTTGGCAAA AAAAGAAGGT 
ATCACAGTGC CTCCTCCAGA CTCTTCGAAA CCCATGGGGC CCAATGATGC CATAGATGCT 
TTGTCATCTG ACTTCACCTG CAGTTCACCT ACCGATGCTG GAAAGAAAAC TAAGGAAGAG 
AAATCTACAG GAGAAGAGAT TTTAAAAGCC CAGCCAGCCA GGGTAACCAA AAGTGCTGCT 
TCACCCCAAG AGAAAAAAAG AAAGGTGGAG GAGCAGGACG CAATGAGTGA TCAAGCACTG 
GAGGCTCTTT CAGCTTCTTT GGGCAGCCGG AAGCCAGAAC CCGAGCTGGA CCTCAGTTCC 
ATTAAGGAAG TCGACGAGGC AAAAGCCAAA GAAGAGAAAC TAAAGAAGTG TGGTGAAGAT 
GAGGAAACTG TCCCATCAGA GTACAGATTA AAACCAGCTA CGGATAAAGA TGGAAAACCT 
CTATTGCCAG AGCCTGAAGA AAAATCCAAG CCCCTGAGTG AATCAGAACT CATTGATGAG 
CTTTCAGAAG ATTTTAACCA GCCTAAAAGT AAAGGAGAAC AGTCTAAGCC AACTGAAAAA 
ACAGAAGAAT CTCAGGCTGC CCCCCTAACT CCTGTGAGCG AGGCTGTGCC TCGGACCTCC 
ATGTGTAGCA TACAGTCGGC ACCGCCCAAG CCACCTACCT TGGAAGGCAT GGTGCCAGAT 
GATGCTGTAG AAGCCTTGGC TGGTAGCCTG GGGAAGAAGG AAGCAGATCC AGGAGATGGA 
AAGCCTGTGG AAGATAAAGT CAAGGAGAAA GCCAAGGAAG AGGATCGTGA AAAACTTGGT 
GAAAAAGAAG AAACAATTCC TCCTGATTAT AGATTAGAAG AGGTCAAGGA TAAAGATGGA 
AAACCACTCC TGCCAAAAGT GCCCAAGGAA TCGCTTTTAC CCATGAGTGA AGACTTCCTT 
CTTGATGCTT TGTCCAAGGA CTTTGCTGGT TCCTCAAACA CTTCACCTCT TGAATTTGAA 
GAGGCTAACC TTCCTGCTGT CATCTCTGAA GTGGTTTCCC AAACCCCAGC TCCAACCACG 
CACTCTGCTG GTCTACCCCC TGATACTGTG CAAAGTGACA ACAAAGAACT GGACGATGCT 
TTGGACCAAC TTTCTGACAG TCTGGGACAA AGGCAGCCTG ATCCAGATGA GAACAAGCCG 
ATAGAGGATA AAGTCAAGGA AAGAGCTAAA GCTGAACACA GAGACAAGCT GGGAGAAAGA 
GACGACACCA TCCCCCCTGA ATATAGACAC CTCTTGGATA ACGATGAGGG TAAACCAGGA 
AAGCCACCTG CCAAGAAAAC CAAGGACTCA AAGAAGCCTG AAGATGACAA AGACCCCATC 
GATGCCCTGT CAGGCGACTT CGACAGCTGT CCTGCACCCA CAGAAGCCTC AGAGAACACA 
GCAGAGGACA AAGCCAAGAG AACTGCTCCC AGCTCCAAAA CACCGAAGAA TAGGGGTAAA 
GCAAAGGATT CTGCAAAGGC AAAGCAGGAA ACTTCCAAGC CCAAAGCTGA TGAAAAAAAT 
ACAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005618131.2
CDS	102..2510
Translation	MSQPGQKPAASPRPRRAAAARDTQEHVSEKTSGSPSKSGEKKGADEKKARSLESSQPSRTHAGGTAPATKASASSASTSKSSSMNPTETKAIPVSKQTGGQHSPNKKRHKKQAAKTEPEKKSQSTKPSVVHEKKTQEVKPKEHKEPKSLPKHASDMGSKHVHSEKAASRSSEQLTSEKSTKPKTKAQDSVSAGGESVVAGAAAAAASATAANKKKEKKSLTSAVPVESKPGKPPGKSDMDAALDDLVDTLGKPEETKEDNVPYTGPEISDPMTSTYIEELGKREVTLPPKYRELLAKKEGITVPPPDSSKPMGPNDAIDALSSDFTCSSPTDAGKKTKEEKSTGEEILKAQPARVTKSAASPQEKKRKVEEQDAMSDQALEALSASLGSRKPEPELDLSSIKEVDEAKAKEEKLKKCGEDEETVPSEYRLKPATDKDGKPLLPEPEEKSKPLSESELIDELSEDFNQPKSKGEQSKPTEKTEESQAAPLTPVSEAVPRTSMCSIQSAPPKPPTLEGMVPDDAVEALAGSLGKKEADPGDGKPVEDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKVPKESLLPMSEDFLLDALSKDFAGSSNTSPLEFEEANLPAVISEVVSQTPAPTTHSAGLPPDTVQSDNKELDDALDQLSDSLGQRQPDPDENKPIEDKVKERAKAEHRDKLGERDDTIPPEYRHLLDNDEGKPGKPPAKKTKDSKKPEDDKDPIDALSGDFDSCPAPTEASENTAEDKAKRTAPSSKTPKNRGKAKDSAKAKQETSKPKADEKNTS

Target ORF information:

RefSeq Version	XM_005618074.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calpastatin (CAST), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005618074.2

ORF Insert Sequence:

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTATTCCAG TCAGCAAACA GACGGGAGGA 
CAGCATTCTC CTAACAAGAA AAGACACAAA AAACAGGCTG CAAAAACAGA ACCTGAAAAG 
AAGTCACAAT CAACTAAGCC ATCTGTGGTC CATGAAAAAA AAACCCAAGA AGTAAAGCCA 
AAGGAACACA AAGAGCCAAA AAGCCTACCC AAGCATGCAT CAGATATGGG AAGCAAGCAT 
GTTCATAGTG AAAAAGCAGC TTCTAGATCA AGTGAGCAGC TGACATCAGA GAAATCAACA 
AAACCAAAGA CTAAAGCACA GGATTCCGTT TCTGCCGGTG GGGAGAGTGT TGTTGCAGGT 
GCAGCTGCAG CTGCAGCATC TGCCACAGCA GCTAACAAGA AAAAAGAAAA GAAATCACTA 
ACATCGGCTG TACCAGTTGA ATCCAAACCG GGTAAACCAC CTGGAAAGTC AGACATGGAT 
GCTGCTTTGG ATGACTTAGT AGACACTCTA GGAAAACCTG AAGAAACTAA AGAAGATAAT 
GTACCATATA CTGGACCTGA AATTTCAGAT CCGATGACTT CCACCTACAT AGAGGAATTA 
GGTAAAAGAG AAGTCACACT TCCTCCAAAG TACAGGGAAC TTTTGGCAAA AAAAGAAGGT 
ATCACAGTGC CTCCTCCAGA CTCTTCGAAA CCCATGGGGC CCAATGATGC CATAGATGCT 
TTGTCATCTG ACTTCACCTG CAGTTCACCT ACCGATGCTG GAAAGAAAAC TAAGGAAGAG 
AAATCTACAG GAGAAGAGAT TTTAAAAGCC CAGCCAGCCA GGGTAACCAA AAGTGCTGCT 
TCACCCCAAG AGAAAAAAAG AAAGGTGGAG GAGCAGGACG CAATGAGTGA TCAAGCACTG 
GAGGCTCTTT CAGCTTCTTT GGGCAGCCGG AAGCCAGAAC CCGAGCTGGA CCTCAGTTCC 
ATTAAGGAAG TCGACGAGGC AAAAGCCAAA GAAGAGAAAC TAAAGAAGTG TGGTGAAGAT 
GAGGAAACTG TCCCATCAGA GTACAGATTA AAACCAGCTA CGGATAAAGA TGGAAAACCT 
CTATTGCCAG AGCCTGAAGA AAAATCCAAG CCCCTGAGTG AATCAGAACT CATTGATGAG 
CTTTCAGAAG ATTTTAACCA GCCTAAAAGT AAAGGAGAAC AGTCTAAGCC AACTGAAAAA 
ACAGAAGAAT CTCAGGCTGC CCCCCTAACT CCTGTGAGCG AGGCTGTGCC TCGGACCTCC 
ATGTGTAGCA TACAGTCGGC ACCGCCCAAG CCACCTACCT TGGAAGGCAT GGTGCCAGAT 
GATGCTGTAG AAGCCTTGGC TGGTAGCCTG GGGAAGAAGG AAGCAGATCC AGGAGATGGA 
AAGCCTGTGG AAGATAAAGT CAAGGAGAAA GCCAAGGAAG AGGATCGTGA AAAACTTGGT 
GAAAAAGAAG AAACAATTCC TCCTGATTAT AGATTAGAAG AGGTCAAGGA TAAAGATGGA 
AAACCACTCC TGCCAAAAGT GCCCAAGGAA TCGCTTTTAC CCATGAGTGA AGACTTCCTT 
CTTGATGCTT TGTCCAAGGA CTTTGCTGGT TCCTCAAACA CTTCACCTCT TGAATTTGAA 
GAGGCTAACC TTCCTGCTGT CATCTCTGAA GTGGTTTCCC AAACCCCAGC TCCAACCACG 
CACTCTGCTG GTCTACCCCC TGATACTGTG CAAAGTGACA ACAAAGAACT GGACGATGCT 
TTGGACCAAC TTTCTGACAG TCTGGGACAA AGGCAGCCTG ATCCAGATGA GAACAAGCCG 
ATAGAGGATA AAGTCAAGGA AAGAGCTAAA GCTGAACACA GAGACAAGCT GGGAGAAAGA 
GACGACACCA TCCCCCCTGA ATATAGACAC CTCTTGGATA ACGATGAGGG TAAACCAGGA 
AAGCCACCTG CCAAGAAAAC CAAGGACTCA AAGAAGCCTG AAGATGACAA AGACCCCATC 
GATGCCCTGT CAGGCGACTT CGACAGCTGT CCTGCACCCA CAGAAGCCTC AGAGAACACA 
GCAGAGGACA AAGCCAAGAG AACTGCTCCC AGCTCCAAAA CACCGAAGAA TAGGGGTAAA 
GCAAAGGATT CTGCAAAGGC AAAGCAGGAA ACTTCCAAGC CCAAAGCTGA TGAAAAAAAT 
ACAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_014112195.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013967670.1 Canis lupus familiaris calpastatin (CAST), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe35582
Clone ID Related Accession (Same CDS sequence)	XM_014112195.2 , XM_014112195.1
Accession Version	XM_014112195.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	calpastatin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014112195.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTATTCCAG TCAGCAAACA GACGGGAGGA 
CAGCATTCTC CTAACAAGAA AAGACACAAA AAACAGGCTG CAAAAACAGA ACCTGAAAAG 
AAGTCACAAT CAACTAAGCC ATCTGTGGTC CATGAAAAAA AAACCCAAGA AGTAAAGCCA 
AAGGAACACA AAGAGCCAAA AAGCCTACCC AAGCATGCAT CAGATATGGG AAGCAAGCAT 
GTTCATAGTG AAAAAGCAGC TTCTAGATCA AGTGAGCAGC TGACATCAGA GAAATCAACA 
AAACCAAAGA CTAAAGCACA GGATTCCGTT TCTGCCGGTG GGGAGAGTGT TGTTGCAGGT 
GCAGCTGCAG CTGCAGCATC TGCCACAGCA GCTAACAAGC AGAAAAAAGA AAAGAAATCA 
CTAACATCGG CTGTACCAGT TGAATCCAAA CCGGGTAAAC CACCTGGAAA GTCAGACATG 
GATGCTGCTT TGGATGACTT AGTAGACACT CTAGGAAAAC CTGAAGAAAC TAAAGAAGAT 
AATGTACCAT ATACTGGACC TGAAATTTCA GATCCGATGA CTTCCACCTA CATAGAGGAA 
TTAGGTAAAA GAGAAGTCAC ACTTCCTCCA AAGTACAGGG AACTTTTGGC AAAAAAAGAA 
GGTATCACAG TGCCTCCTCC AGACTCTTCG AAACCCATGG GGCCCAATGA TGCCATAGAT 
GCTTTGTCAT CTGACTTCAC CTGCAGTTCA CCTACCGATG CTGGAAAGAA AACTAAGGAA 
GAGAAATCTA CAGGAGAAGA GATTTTAAAA GCCCAGCCAG CCAGGGTAAC CAAAAGTGCT 
GCTTCACCCC AAGAGAAAAA AAGAAAGGTG GAGGAGGACG CAATGAGTGA TCAAGCACTG 
GAGGCTCTTT CAGCTTCTTT GGGCAGCCGG AAGCCAGAAC CCGAGCTGGA CCTCAGTTCC 
ATTAAGGAAG TCGACGAGGC AAAAGCCAAA GAAGAGAAAC TAAAGAAGTG TGGTGAAGAT 
GAGGAAACTG TCCCATCAGA GTACAGATTA AAACCAGCTA CGGATAAAGA TGGAAAACCT 
CTATTGCCAG AGCCTGAAGA AAAATCCAAG CCCCTGAGTG AATCAGAACT CATTGATGAG 
CTTTCAGAAG ATTTTAACCA GCCTAAAAGT AAAGGAGAAC AGTCTAAGCC AACTGAAAAA 
ACAGAAGAAT CTCAGGCTGC CCCCCTAACT CCTGTGAGCG AGGCTGTGCC TCGGACCTCC 
ATGTGTAGCA TACAGTCGGC ACCGCCCAAG CCACCTACCT TGGAAGGCAT GGTGCCAGAT 
GATGCTGTAG AAGCCTTGGC TGGTAGCCTG GGGAAGAAGG AAGCAGATCC AGGAGATGGA 
AAGCCTGTGG AAGATAAAGT CAAGGAGAAA GCCAAGGAAG AGGATCGTGA AAAACTTGGT 
GAAAAAGAAG AAACAATTCC TCCTGATTAT AGATTAGAAG AGGTCAAGGA TAAAGATGGA 
AAACCACTCC TGCCAAAAGT GCCCAAGGAA TCGCTTTTAC CCATGAGTGA AGACTTCCTT 
CTTGATGCTT TGTCCAAGGA CTTTGCTGGT TCCTCAAACA CTTCACCTCT TGAATTTGAA 
GAGGCTAACC TTCCTGCTGT CATCTCTGAA GTGGTTTCCC AAACCCCAGC TCCAACCACG 
CACTCTGCTG GTCTACCCCC TGATACTGTG CAAAGTGACA ACAAAGAACT GGACGATGCT 
TTGGACCAAC TTTCTGACAG TCTGGGACAA AGGCAGCCTG ATCCAGATGA GAACAAGCCG 
ATAGAGGATA AAGTCAAGGA AAGAGCTAAA GCTGAACACA GAGACAAGCT GGGAGAAAGA 
GACGACACCA TCCCCCCTGA ATATAGACAC CTCTTGGATA ACGATGAGGG TAAACCAGGA 
AAGCCACCTG CCAAGAAAAC CAAGGACTCA AAGAAGCCTG AAGATGACAA AGACCCCATC 
GATGCCCTGT CAGGCGACTT CGACAGCTGT CCTGCACCCA CAGAAGCCTC AGAGAACACA 
GCAGAGGACA AAGCCAAGAG AACTGCTCCC AGCTCCAAAA CACCGAAGAA TAGGGGTAAA 
GCAAAGGATT CTGCAAAGGC AAAGCAGGAA ACTTCCAAGC CCAAAGCTGA TGAAAAAAAT 
ACAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967670.1
CDS	79..2487
Translation	MSQPGQKPAASPRPRRAAAARDTQEHVSEKTSGSPSKSGEKKGADEKKARSLESSQPSRTHAGGTAPATKASASSASTSKSSSMNPTETKAIPVSKQTGGQHSPNKKRHKKQAAKTEPEKKSQSTKPSVVHEKKTQEVKPKEHKEPKSLPKHASDMGSKHVHSEKAASRSSEQLTSEKSTKPKTKAQDSVSAGGESVVAGAAAAAASATAANKQKKEKKSLTSAVPVESKPGKPPGKSDMDAALDDLVDTLGKPEETKEDNVPYTGPEISDPMTSTYIEELGKREVTLPPKYRELLAKKEGITVPPPDSSKPMGPNDAIDALSSDFTCSSPTDAGKKTKEEKSTGEEILKAQPARVTKSAASPQEKKRKVEEDAMSDQALEALSASLGSRKPEPELDLSSIKEVDEAKAKEEKLKKCGEDEETVPSEYRLKPATDKDGKPLLPEPEEKSKPLSESELIDELSEDFNQPKSKGEQSKPTEKTEESQAAPLTPVSEAVPRTSMCSIQSAPPKPPTLEGMVPDDAVEALAGSLGKKEADPGDGKPVEDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKVPKESLLPMSEDFLLDALSKDFAGSSNTSPLEFEEANLPAVISEVVSQTPAPTTHSAGLPPDTVQSDNKELDDALDQLSDSLGQRQPDPDENKPIEDKVKERAKAEHRDKLGERDDTIPPEYRHLLDNDEGKPGKPPAKKTKDSKKPEDDKDPIDALSGDFDSCPAPTEASENTAEDKAKRTAPSSKTPKNRGKAKDSAKAKQETSKPKADEKNTS

Target ORF information:

RefSeq Version	XM_014112195.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calpastatin (CAST), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014112195.2

ORF Insert Sequence:

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTATTCCAG TCAGCAAACA GACGGGAGGA 
CAGCATTCTC CTAACAAGAA AAGACACAAA AAACAGGCTG CAAAAACAGA ACCTGAAAAG 
AAGTCACAAT CAACTAAGCC ATCTGTGGTC CATGAAAAAA AAACCCAAGA AGTAAAGCCA 
AAGGAACACA AAGAGCCAAA AAGCCTACCC AAGCATGCAT CAGATATGGG AAGCAAGCAT 
GTTCATAGTG AAAAAGCAGC TTCTAGATCA AGTGAGCAGC TGACATCAGA GAAATCAACA 
AAACCAAAGA CTAAAGCACA GGATTCCGTT TCTGCCGGTG GGGAGAGTGT TGTTGCAGGT 
GCAGCTGCAG CTGCAGCATC TGCCACAGCA GCTAACAAGC AGAAAAAAGA AAAGAAATCA 
CTAACATCGG CTGTACCAGT TGAATCCAAA CCGGGTAAAC CACCTGGAAA GTCAGACATG 
GATGCTGCTT TGGATGACTT AGTAGACACT CTAGGAAAAC CTGAAGAAAC TAAAGAAGAT 
AATGTACCAT ATACTGGACC TGAAATTTCA GATCCGATGA CTTCCACCTA CATAGAGGAA 
TTAGGTAAAA GAGAAGTCAC ACTTCCTCCA AAGTACAGGG AACTTTTGGC AAAAAAAGAA 
GGTATCACAG TGCCTCCTCC AGACTCTTCG AAACCCATGG GGCCCAATGA TGCCATAGAT 
GCTTTGTCAT CTGACTTCAC CTGCAGTTCA CCTACCGATG CTGGAAAGAA AACTAAGGAA 
GAGAAATCTA CAGGAGAAGA GATTTTAAAA GCCCAGCCAG CCAGGGTAAC CAAAAGTGCT 
GCTTCACCCC AAGAGAAAAA AAGAAAGGTG GAGGAGGACG CAATGAGTGA TCAAGCACTG 
GAGGCTCTTT CAGCTTCTTT GGGCAGCCGG AAGCCAGAAC CCGAGCTGGA CCTCAGTTCC 
ATTAAGGAAG TCGACGAGGC AAAAGCCAAA GAAGAGAAAC TAAAGAAGTG TGGTGAAGAT 
GAGGAAACTG TCCCATCAGA GTACAGATTA AAACCAGCTA CGGATAAAGA TGGAAAACCT 
CTATTGCCAG AGCCTGAAGA AAAATCCAAG CCCCTGAGTG AATCAGAACT CATTGATGAG 
CTTTCAGAAG ATTTTAACCA GCCTAAAAGT AAAGGAGAAC AGTCTAAGCC AACTGAAAAA 
ACAGAAGAAT CTCAGGCTGC CCCCCTAACT CCTGTGAGCG AGGCTGTGCC TCGGACCTCC 
ATGTGTAGCA TACAGTCGGC ACCGCCCAAG CCACCTACCT TGGAAGGCAT GGTGCCAGAT 
GATGCTGTAG AAGCCTTGGC TGGTAGCCTG GGGAAGAAGG AAGCAGATCC AGGAGATGGA 
AAGCCTGTGG AAGATAAAGT CAAGGAGAAA GCCAAGGAAG AGGATCGTGA AAAACTTGGT 
GAAAAAGAAG AAACAATTCC TCCTGATTAT AGATTAGAAG AGGTCAAGGA TAAAGATGGA 
AAACCACTCC TGCCAAAAGT GCCCAAGGAA TCGCTTTTAC CCATGAGTGA AGACTTCCTT 
CTTGATGCTT TGTCCAAGGA CTTTGCTGGT TCCTCAAACA CTTCACCTCT TGAATTTGAA 
GAGGCTAACC TTCCTGCTGT CATCTCTGAA GTGGTTTCCC AAACCCCAGC TCCAACCACG 
CACTCTGCTG GTCTACCCCC TGATACTGTG CAAAGTGACA ACAAAGAACT GGACGATGCT 
TTGGACCAAC TTTCTGACAG TCTGGGACAA AGGCAGCCTG ATCCAGATGA GAACAAGCCG 
ATAGAGGATA AAGTCAAGGA AAGAGCTAAA GCTGAACACA GAGACAAGCT GGGAGAAAGA 
GACGACACCA TCCCCCCTGA ATATAGACAC CTCTTGGATA ACGATGAGGG TAAACCAGGA 
AAGCCACCTG CCAAGAAAAC CAAGGACTCA AAGAAGCCTG AAGATGACAA AGACCCCATC 
GATGCCCTGT CAGGCGACTT CGACAGCTGT CCTGCACCCA CAGAAGCCTC AGAGAACACA 
GCAGAGGACA AAGCCAAGAG AACTGCTCCC AGCTCCAAAA CACCGAAGAA TAGGGGTAAA 
GCAAAGGATT CTGCAAAGGC AAAGCAGGAA ACTTCCAAGC CCAAAGCTGA TGAAAAAAAT 
ACAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_014112195.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013967670.1 Canis lupus familiaris calpastatin (CAST), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe35582
Clone ID Related Accession (Same CDS sequence)	XM_014112195.2 , XM_014112195.1
Accession Version	XM_014112195.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	calpastatin isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726052.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTATTCCAG TCAGCAAACA GACGGGAGGA 
CAGCATTCTC CTAACAAGAA AAGACACAAA AAACAGGCTG CAAAAACAGA ACCTGAAAAG 
AAGTCACAAT CAACTAAGCC ATCTGTGGTC CATGAAAAAA AAACCCAAGA AGTAAAGCCA 
AAGGAACACA AAGAGCCAAA AAGCCTACCC AAGCATGCAT CAGATATGGG AAGCAAGCAT 
GTTCATAGTG AAAAAGCAGC TTCTAGATCA AGTGAGCAGC TGACATCAGA GAAATCAACA 
AAACCAAAGA CTAAAGCACA GGATTCCGTT TCTGCCGGTG GGGAGAGTGT TGTTGCAGGT 
GCAGCTGCAG CTGCAGCATC TGCCACAGCA GCTAACAAGC AGAAAAAAGA AAAGAAATCA 
CTAACATCGG CTGTACCAGT TGAATCCAAA CCGGGTAAAC CACCTGGAAA GTCAGACATG 
GATGCTGCTT TGGATGACTT AGTAGACACT CTAGGAAAAC CTGAAGAAAC TAAAGAAGAT 
AATGTACCAT ATACTGGACC TGAAATTTCA GATCCGATGA CTTCCACCTA CATAGAGGAA 
TTAGGTAAAA GAGAAGTCAC ACTTCCTCCA AAGTACAGGG AACTTTTGGC AAAAAAAGAA 
GGTATCACAG TGCCTCCTCC AGACTCTTCG AAACCCATGG GGCCCAATGA TGCCATAGAT 
GCTTTGTCAT CTGACTTCAC CTGCAGTTCA CCTACCGATG CTGGAAAGAA AACTAAGGAA 
GAGAAATCTA CAGGAGAAGA GATTTTAAAA GCCCAGCCAG CCAGGGTAAC CAAAAGTGCT 
GCTTCACCCC AAGAGAAAAA AAGAAAGGTG GAGGAGGACG CAATGAGTGA TCAAGCACTG 
GAGGCTCTTT CAGCTTCTTT GGGCAGCCGG AAGCCAGAAC CCGAGCTGGA CCTCAGTTCC 
ATTAAGGAAG TCGACGAGGC AAAAGCCAAA GAAGAGAAAC TAAAGAAGTG TGGTGAAGAT 
GAGGAAACTG TCCCATCAGA GTACAGATTA AAACCAGCTA CGGATAAAGA TGGAAAACCT 
CTATTGCCAG AGCCTGAAGA AAAATCCAAG CCCCTGAGTG AATCAGAACT CATTGATGAG 
CTTTCAGAAG ATTTTAACCA GCCTAAAAGT AAAGGAGAAC AGTCTAAGCC AACTGAAAAA 
ACAGAAGAAT CTCAGGCTGC CCCCCTAACT CCTGTGAGCG AGGCTGTGCC TCGGACCTCC 
ATGTGTAGCA TACAGTCGGC ACCGCCCAAG CCACCTACCT TGGAAGGCAT GGTGCCAGAT 
GATGCTGTAG AAGCCTTGGC TGGTAGCCTG GGGAAGAAGG AAGCAGATCC AGGAGATGGA 
AAGCCTGTGG AAGATAAAGT CAAGGAGAAA GCCAAGGAAG AGGATCGTGA AAAACTTGGT 
GAAAAAGAAG AAACAATTCC TCCTGATTAT AGATTAGAAG AGGTCAAGGA TAAAGATGGA 
AAACCACTCC TGCCAAAAGT GCCCAAGGAA TCGCTTTTAC CCATGAGTGA AGACTTCCTT 
CTTGATGCTT TGTCCAAGGA CTTTGCTGGT TCCTCAAACA CTTCACCTCT TGAATTTGAA 
GAGGCTAACC TTCCTGCTGT CATCTCTGAA GTGGTTTCCC AAACCCCAGC TCCAACCACG 
CACTCTGCTG GTCTACCCCC TGATACTGTG CAAAGTGACA ACAAAGAACT GGACGATGCT 
TTGGACCAAC TTTCTGACAG TCTGGGACAA AGGCAGCCTG ATCCAGATGA GAACAAGCCG 
ATAGAGGATA AAGTCAAGGA AAGAGCTAAA GCTGAACACA GAGACAAGCT GGGAGAAAGA 
GACGACACCA TCCCCCCTGA ATATAGACAC CTCTTGGATA ACGATGAGGG TAAACCAGGA 
AAGCCACCTG CCAAGAAAAC CAAGGACTCA AAGAAGCCTG AAGATGACAA AGACCCCATC 
GATGCCCTGT CAGGCGACTT CGACAGCTGT CCTGCACCCA CAGAAGCCTC AGAGAACACA 
GCAGAGGACA AAGCCAAGAG AACTGCTCCC AGCTCCAAAA CACCGAAGAA TAGGGGTAAA 
GCAAAGGATT CTGCAAAGGC AAAGCAGGAA ACTTCCAAGC CCAAAGCTGA TGAAAAAAAT 
ACAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967670.1
CDS	102..2510
Translation	MSQPGQKPAASPRPRRAAAARDTQEHVSEKTSGSPSKSGEKKGADEKKARSLESSQPSRTHAGGTAPATKASASSASTSKSSSMNPTETKAIPVSKQTGGQHSPNKKRHKKQAAKTEPEKKSQSTKPSVVHEKKTQEVKPKEHKEPKSLPKHASDMGSKHVHSEKAASRSSEQLTSEKSTKPKTKAQDSVSAGGESVVAGAAAAAASATAANKQKKEKKSLTSAVPVESKPGKPPGKSDMDAALDDLVDTLGKPEETKEDNVPYTGPEISDPMTSTYIEELGKREVTLPPKYRELLAKKEGITVPPPDSSKPMGPNDAIDALSSDFTCSSPTDAGKKTKEEKSTGEEILKAQPARVTKSAASPQEKKRKVEEDAMSDQALEALSASLGSRKPEPELDLSSIKEVDEAKAKEEKLKKCGEDEETVPSEYRLKPATDKDGKPLLPEPEEKSKPLSESELIDELSEDFNQPKSKGEQSKPTEKTEESQAAPLTPVSEAVPRTSMCSIQSAPPKPPTLEGMVPDDAVEALAGSLGKKEADPGDGKPVEDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKVPKESLLPMSEDFLLDALSKDFAGSSNTSPLEFEEANLPAVISEVVSQTPAPTTHSAGLPPDTVQSDNKELDDALDQLSDSLGQRQPDPDENKPIEDKVKERAKAEHRDKLGERDDTIPPEYRHLLDNDEGKPGKPPAKKTKDSKKPEDDKDPIDALSGDFDSCPAPTEASENTAEDKAKRTAPSSKTPKNRGKAKDSAKAKQETSKPKADEKNTS

Target ORF information:

RefSeq Version	XM_014112195.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calpastatin (CAST), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014112195.1

ORF Insert Sequence:

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTATTCCAG TCAGCAAACA GACGGGAGGA 
CAGCATTCTC CTAACAAGAA AAGACACAAA AAACAGGCTG CAAAAACAGA ACCTGAAAAG 
AAGTCACAAT CAACTAAGCC ATCTGTGGTC CATGAAAAAA AAACCCAAGA AGTAAAGCCA 
AAGGAACACA AAGAGCCAAA AAGCCTACCC AAGCATGCAT CAGATATGGG AAGCAAGCAT 
GTTCATAGTG AAAAAGCAGC TTCTAGATCA AGTGAGCAGC TGACATCAGA GAAATCAACA 
AAACCAAAGA CTAAAGCACA GGATTCCGTT TCTGCCGGTG GGGAGAGTGT TGTTGCAGGT 
GCAGCTGCAG CTGCAGCATC TGCCACAGCA GCTAACAAGC AGAAAAAAGA AAAGAAATCA 
CTAACATCGG CTGTACCAGT TGAATCCAAA CCGGGTAAAC CACCTGGAAA GTCAGACATG 
GATGCTGCTT TGGATGACTT AGTAGACACT CTAGGAAAAC CTGAAGAAAC TAAAGAAGAT 
AATGTACCAT ATACTGGACC TGAAATTTCA GATCCGATGA CTTCCACCTA CATAGAGGAA 
TTAGGTAAAA GAGAAGTCAC ACTTCCTCCA AAGTACAGGG AACTTTTGGC AAAAAAAGAA 
GGTATCACAG TGCCTCCTCC AGACTCTTCG AAACCCATGG GGCCCAATGA TGCCATAGAT 
GCTTTGTCAT CTGACTTCAC CTGCAGTTCA CCTACCGATG CTGGAAAGAA AACTAAGGAA 
GAGAAATCTA CAGGAGAAGA GATTTTAAAA GCCCAGCCAG CCAGGGTAAC CAAAAGTGCT 
GCTTCACCCC AAGAGAAAAA AAGAAAGGTG GAGGAGGACG CAATGAGTGA TCAAGCACTG 
GAGGCTCTTT CAGCTTCTTT GGGCAGCCGG AAGCCAGAAC CCGAGCTGGA CCTCAGTTCC 
ATTAAGGAAG TCGACGAGGC AAAAGCCAAA GAAGAGAAAC TAAAGAAGTG TGGTGAAGAT 
GAGGAAACTG TCCCATCAGA GTACAGATTA AAACCAGCTA CGGATAAAGA TGGAAAACCT 
CTATTGCCAG AGCCTGAAGA AAAATCCAAG CCCCTGAGTG AATCAGAACT CATTGATGAG 
CTTTCAGAAG ATTTTAACCA GCCTAAAAGT AAAGGAGAAC AGTCTAAGCC AACTGAAAAA 
ACAGAAGAAT CTCAGGCTGC CCCCCTAACT CCTGTGAGCG AGGCTGTGCC TCGGACCTCC 
ATGTGTAGCA TACAGTCGGC ACCGCCCAAG CCACCTACCT TGGAAGGCAT GGTGCCAGAT 
GATGCTGTAG AAGCCTTGGC TGGTAGCCTG GGGAAGAAGG AAGCAGATCC AGGAGATGGA 
AAGCCTGTGG AAGATAAAGT CAAGGAGAAA GCCAAGGAAG AGGATCGTGA AAAACTTGGT 
GAAAAAGAAG AAACAATTCC TCCTGATTAT AGATTAGAAG AGGTCAAGGA TAAAGATGGA 
AAACCACTCC TGCCAAAAGT GCCCAAGGAA TCGCTTTTAC CCATGAGTGA AGACTTCCTT 
CTTGATGCTT TGTCCAAGGA CTTTGCTGGT TCCTCAAACA CTTCACCTCT TGAATTTGAA 
GAGGCTAACC TTCCTGCTGT CATCTCTGAA GTGGTTTCCC AAACCCCAGC TCCAACCACG 
CACTCTGCTG GTCTACCCCC TGATACTGTG CAAAGTGACA ACAAAGAACT GGACGATGCT 
TTGGACCAAC TTTCTGACAG TCTGGGACAA AGGCAGCCTG ATCCAGATGA GAACAAGCCG 
ATAGAGGATA AAGTCAAGGA AAGAGCTAAA GCTGAACACA GAGACAAGCT GGGAGAAAGA 
GACGACACCA TCCCCCCTGA ATATAGACAC CTCTTGGATA ACGATGAGGG TAAACCAGGA 
AAGCCACCTG CCAAGAAAAC CAAGGACTCA AAGAAGCCTG AAGATGACAA AGACCCCATC 
GATGCCCTGT CAGGCGACTT CGACAGCTGT CCTGCACCCA CAGAAGCCTC AGAGAACACA 
GCAGAGGACA AAGCCAAGAG AACTGCTCCC AGCTCCAAAA CACCGAAGAA TAGGGGTAAA 
GCAAAGGATT CTGCAAAGGC AAAGCAGGAA ACTTCCAAGC CCAAAGCTGA TGAAAAAAAT 
ACAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_014112197.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013967672.1 Canis lupus familiaris calpastatin (CAST), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe35584
Clone ID Related Accession (Same CDS sequence)	XM_014112197.1 , XM_014112197.2
Accession Version	XM_014112197.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	calpastatin isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726052.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTATTCCAG TCAGCAAACA GACGGGAGGA 
CAGCATTCTC CTAACAAGAA AAGACACAAA AAACAGGCTG CAAAAACAGA ACCTGAAAAG 
AAGTCACAAT CAACTAAGCC AAAAAGCCTA CCCAAGCATG CATCAGATAT GGGAAGCAAG 
CATGTTCATA GTGAAAAAGC AGCTTCTAGA TCAAGTGAGC AGCTGACATC AGAGAAATCA 
ACAAAACCAA AGACTAAAGC ACAGGATTCC GTTTCTGCCG GTGGGGAGAG TGTTGTTGCA 
GGTGCAGCTG CAGCTGCAGC ATCTGCCACA GCAGCTAACA AGCAGAAAAA AGAAAAGAAA 
TCACTAACAT CGGCTGTACC AGTTGAATCC AAACCGGGTA AACCACCTGG AAAGTCAGAC 
ATGGATGCTG CTTTGGATGA CTTAGTAGAC ACTCTAGGAA AACCTGAAGA AACTAAAGAA 
GATAATGTAC CATATACTGG ACCTGAAATT TCAGATCCGA TGACTTCCAC CTACATAGAG 
GAATTAGGTA AAAGAGAAGT CACACTTCCT CCAAAGTACA GGGAACTTTT GGCAAAAAAA 
GAAGGTATCA CAGTGCCTCC TCCAGACTCT TCGAAACCCA TGGGGCCCAA TGATGCCATA 
GATGCTTTGT CATCTGACTT CACCTGCAGT TCACCTACCG ATGCTGGAAA GAAAACTAAG 
GAAGAGAAAT CTACAGGAGA AGAGATTTTA AAAGCCCAGC CAGCCAGGGT AACCAAAAGT 
GCTGCTTCAC CCCAAGAGAA AAAAAGAAAG GTGGAGGAGC AGGACGCAAT GAGTGATCAA 
GCACTGGAGG CTCTTTCAGC TTCTTTGGGC AGCCGGAAGC CAGAACCCGA GCTGGACCTC 
AGTTCCATTA AGGAAGTCGA CGAGGCAAAA GCCAAAGAAG AGAAACTAAA GAAGTGTGGT 
GAAGATGAGG AAACTGTCCC ATCAGAGTAC AGATTAAAAC CAGCTACGGA TAAAGATGGA 
AAACCTCTAT TGCCAGAGCC TGAAGAAAAA TCCAAGCCCC TGAGTGAATC AGAACTCATT 
GATGAGCTTT CAGAAGATTT TAACCAGCCT AAAAGTAAAG GAGAACAGTC TAAGCCAACT 
GAAAAAACAG AAGAATCTCA GGCTGCCCCC CTAACTCCTG TGAGCGAGGC TGTGCCTCGG 
ACCTCCATGT GTAGCATACA GTCGGCACCG CCCAAGCCAC CTACCTTGGA AGGCATGGTG 
CCAGATGATG CTGTAGAAGC CTTGGCTGGT AGCCTGGGGA AGAAGGAAGC AGATCCAGGA 
GATGGAAAGC CTGTGGAAGA TAAAGTCAAG GAGAAAGCCA AGGAAGAGGA TCGTGAAAAA 
CTTGGTGAAA AAGAAGAAAC AATTCCTCCT GATTATAGAT TAGAAGAGGT CAAGGATAAA 
GATGGAAAAC CACTCCTGCC AAAAGTGCCC AAGGAATCGC TTTTACCCAT GAGTGAAGAC 
TTCCTTCTTG ATGCTTTGTC CAAGGACTTT GCTGGTTCCT CAAACACTTC ACCTCTTGAA 
TTTGAAGAGG CTAACCTTCC TGCTGTCATC TCTGAAGTGG TTTCCCAAAC CCCAGCTCCA 
ACCACGCACT CTGCTGGTCT ACCCCCTGAT ACTGTGCAAA GTGACAACAA AGAACTGGAC 
GATGCTTTGG ACCAACTTTC TGACAGTCTG GGACAAAGGC AGCCTGATCC AGATGAGAAC 
AAGCCGATAG AGGATAAAGT CAAGGAAAGA GCTAAAGCTG AACACAGAGA CAAGCTGGGA 
GAAAGAGACG ACACCATCCC CCCTGAATAT AGACACCTCT TGGATAACGA TGAGGGTAAA 
CCAGGAAAGC CACCTGCCAA GAAAACCAAG GACTCAAAGA AGCCTGAAGA TGACAAAGAC 
CCCATCGATG CCCTGTCAGG CGACTTCGAC AGCTGTCCTG CACCCACAGA AGCCTCAGAG 
AACACAGCAG AGGACAAAGC CAAGAGAACT GCTCCCAGCT CCAAAACACC GAAGAATAGG 
GGTAAAGCAA AGGATTCTGC AAAGGCAAAG CAGGAAACTT CCAAGCCCAA AGCTGATGAA 
AAAAATACAA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967672.1
CDS	102..2456
Translation	MSQPGQKPAASPRPRRAAAARDTQEHVSEKTSGSPSKSGEKKGADEKKARSLESSQPSRTHAGGTAPATKASASSASTSKSSSMNPTETKAIPVSKQTGGQHSPNKKRHKKQAAKTEPEKKSQSTKPKSLPKHASDMGSKHVHSEKAASRSSEQLTSEKSTKPKTKAQDSVSAGGESVVAGAAAAAASATAANKQKKEKKSLTSAVPVESKPGKPPGKSDMDAALDDLVDTLGKPEETKEDNVPYTGPEISDPMTSTYIEELGKREVTLPPKYRELLAKKEGITVPPPDSSKPMGPNDAIDALSSDFTCSSPTDAGKKTKEEKSTGEEILKAQPARVTKSAASPQEKKRKVEEQDAMSDQALEALSASLGSRKPEPELDLSSIKEVDEAKAKEEKLKKCGEDEETVPSEYRLKPATDKDGKPLLPEPEEKSKPLSESELIDELSEDFNQPKSKGEQSKPTEKTEESQAAPLTPVSEAVPRTSMCSIQSAPPKPPTLEGMVPDDAVEALAGSLGKKEADPGDGKPVEDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKVPKESLLPMSEDFLLDALSKDFAGSSNTSPLEFEEANLPAVISEVVSQTPAPTTHSAGLPPDTVQSDNKELDDALDQLSDSLGQRQPDPDENKPIEDKVKERAKAEHRDKLGERDDTIPPEYRHLLDNDEGKPGKPPAKKTKDSKKPEDDKDPIDALSGDFDSCPAPTEASENTAEDKAKRTAPSSKTPKNRGKAKDSAKAKQETSKPKADEKNTS

Target ORF information:

RefSeq Version	XM_014112197.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calpastatin (CAST), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014112197.1

ORF Insert Sequence:

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTATTCCAG TCAGCAAACA GACGGGAGGA 
CAGCATTCTC CTAACAAGAA AAGACACAAA AAACAGGCTG CAAAAACAGA ACCTGAAAAG 
AAGTCACAAT CAACTAAGCC AAAAAGCCTA CCCAAGCATG CATCAGATAT GGGAAGCAAG 
CATGTTCATA GTGAAAAAGC AGCTTCTAGA TCAAGTGAGC AGCTGACATC AGAGAAATCA 
ACAAAACCAA AGACTAAAGC ACAGGATTCC GTTTCTGCCG GTGGGGAGAG TGTTGTTGCA 
GGTGCAGCTG CAGCTGCAGC ATCTGCCACA GCAGCTAACA AGCAGAAAAA AGAAAAGAAA 
TCACTAACAT CGGCTGTACC AGTTGAATCC AAACCGGGTA AACCACCTGG AAAGTCAGAC 
ATGGATGCTG CTTTGGATGA CTTAGTAGAC ACTCTAGGAA AACCTGAAGA AACTAAAGAA 
GATAATGTAC CATATACTGG ACCTGAAATT TCAGATCCGA TGACTTCCAC CTACATAGAG 
GAATTAGGTA AAAGAGAAGT CACACTTCCT CCAAAGTACA GGGAACTTTT GGCAAAAAAA 
GAAGGTATCA CAGTGCCTCC TCCAGACTCT TCGAAACCCA TGGGGCCCAA TGATGCCATA 
GATGCTTTGT CATCTGACTT CACCTGCAGT TCACCTACCG ATGCTGGAAA GAAAACTAAG 
GAAGAGAAAT CTACAGGAGA AGAGATTTTA AAAGCCCAGC CAGCCAGGGT AACCAAAAGT 
GCTGCTTCAC CCCAAGAGAA AAAAAGAAAG GTGGAGGAGC AGGACGCAAT GAGTGATCAA 
GCACTGGAGG CTCTTTCAGC TTCTTTGGGC AGCCGGAAGC CAGAACCCGA GCTGGACCTC 
AGTTCCATTA AGGAAGTCGA CGAGGCAAAA GCCAAAGAAG AGAAACTAAA GAAGTGTGGT 
GAAGATGAGG AAACTGTCCC ATCAGAGTAC AGATTAAAAC CAGCTACGGA TAAAGATGGA 
AAACCTCTAT TGCCAGAGCC TGAAGAAAAA TCCAAGCCCC TGAGTGAATC AGAACTCATT 
GATGAGCTTT CAGAAGATTT TAACCAGCCT AAAAGTAAAG GAGAACAGTC TAAGCCAACT 
GAAAAAACAG AAGAATCTCA GGCTGCCCCC CTAACTCCTG TGAGCGAGGC TGTGCCTCGG 
ACCTCCATGT GTAGCATACA GTCGGCACCG CCCAAGCCAC CTACCTTGGA AGGCATGGTG 
CCAGATGATG CTGTAGAAGC CTTGGCTGGT AGCCTGGGGA AGAAGGAAGC AGATCCAGGA 
GATGGAAAGC CTGTGGAAGA TAAAGTCAAG GAGAAAGCCA AGGAAGAGGA TCGTGAAAAA 
CTTGGTGAAA AAGAAGAAAC AATTCCTCCT GATTATAGAT TAGAAGAGGT CAAGGATAAA 
GATGGAAAAC CACTCCTGCC AAAAGTGCCC AAGGAATCGC TTTTACCCAT GAGTGAAGAC 
TTCCTTCTTG ATGCTTTGTC CAAGGACTTT GCTGGTTCCT CAAACACTTC ACCTCTTGAA 
TTTGAAGAGG CTAACCTTCC TGCTGTCATC TCTGAAGTGG TTTCCCAAAC CCCAGCTCCA 
ACCACGCACT CTGCTGGTCT ACCCCCTGAT ACTGTGCAAA GTGACAACAA AGAACTGGAC 
GATGCTTTGG ACCAACTTTC TGACAGTCTG GGACAAAGGC AGCCTGATCC AGATGAGAAC 
AAGCCGATAG AGGATAAAGT CAAGGAAAGA GCTAAAGCTG AACACAGAGA CAAGCTGGGA 
GAAAGAGACG ACACCATCCC CCCTGAATAT AGACACCTCT TGGATAACGA TGAGGGTAAA 
CCAGGAAAGC CACCTGCCAA GAAAACCAAG GACTCAAAGA AGCCTGAAGA TGACAAAGAC 
CCCATCGATG CCCTGTCAGG CGACTTCGAC AGCTGTCCTG CACCCACAGA AGCCTCAGAG 
AACACAGCAG AGGACAAAGC CAAGAGAACT GCTCCCAGCT CCAAAACACC GAAGAATAGG 
GGTAAAGCAA AGGATTCTGC AAAGGCAAAG CAGGAAACTT CCAAGCCCAA AGCTGATGAA 
AAAAATACAA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_014112197.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013967672.1 Canis lupus familiaris calpastatin (CAST), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe35584
Clone ID Related Accession (Same CDS sequence)	XM_014112197.1 , XM_014112197.2
Accession Version	XM_014112197.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	calpastatin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014112197.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTATTCCAG TCAGCAAACA GACGGGAGGA 
CAGCATTCTC CTAACAAGAA AAGACACAAA AAACAGGCTG CAAAAACAGA ACCTGAAAAG 
AAGTCACAAT CAACTAAGCC AAAAAGCCTA CCCAAGCATG CATCAGATAT GGGAAGCAAG 
CATGTTCATA GTGAAAAAGC AGCTTCTAGA TCAAGTGAGC AGCTGACATC AGAGAAATCA 
ACAAAACCAA AGACTAAAGC ACAGGATTCC GTTTCTGCCG GTGGGGAGAG TGTTGTTGCA 
GGTGCAGCTG CAGCTGCAGC ATCTGCCACA GCAGCTAACA AGCAGAAAAA AGAAAAGAAA 
TCACTAACAT CGGCTGTACC AGTTGAATCC AAACCGGGTA AACCACCTGG AAAGTCAGAC 
ATGGATGCTG CTTTGGATGA CTTAGTAGAC ACTCTAGGAA AACCTGAAGA AACTAAAGAA 
GATAATGTAC CATATACTGG ACCTGAAATT TCAGATCCGA TGACTTCCAC CTACATAGAG 
GAATTAGGTA AAAGAGAAGT CACACTTCCT CCAAAGTACA GGGAACTTTT GGCAAAAAAA 
GAAGGTATCA CAGTGCCTCC TCCAGACTCT TCGAAACCCA TGGGGCCCAA TGATGCCATA 
GATGCTTTGT CATCTGACTT CACCTGCAGT TCACCTACCG ATGCTGGAAA GAAAACTAAG 
GAAGAGAAAT CTACAGGAGA AGAGATTTTA AAAGCCCAGC CAGCCAGGGT AACCAAAAGT 
GCTGCTTCAC CCCAAGAGAA AAAAAGAAAG GTGGAGGAGC AGGACGCAAT GAGTGATCAA 
GCACTGGAGG CTCTTTCAGC TTCTTTGGGC AGCCGGAAGC CAGAACCCGA GCTGGACCTC 
AGTTCCATTA AGGAAGTCGA CGAGGCAAAA GCCAAAGAAG AGAAACTAAA GAAGTGTGGT 
GAAGATGAGG AAACTGTCCC ATCAGAGTAC AGATTAAAAC CAGCTACGGA TAAAGATGGA 
AAACCTCTAT TGCCAGAGCC TGAAGAAAAA TCCAAGCCCC TGAGTGAATC AGAACTCATT 
GATGAGCTTT CAGAAGATTT TAACCAGCCT AAAAGTAAAG GAGAACAGTC TAAGCCAACT 
GAAAAAACAG AAGAATCTCA GGCTGCCCCC CTAACTCCTG TGAGCGAGGC TGTGCCTCGG 
ACCTCCATGT GTAGCATACA GTCGGCACCG CCCAAGCCAC CTACCTTGGA AGGCATGGTG 
CCAGATGATG CTGTAGAAGC CTTGGCTGGT AGCCTGGGGA AGAAGGAAGC AGATCCAGGA 
GATGGAAAGC CTGTGGAAGA TAAAGTCAAG GAGAAAGCCA AGGAAGAGGA TCGTGAAAAA 
CTTGGTGAAA AAGAAGAAAC AATTCCTCCT GATTATAGAT TAGAAGAGGT CAAGGATAAA 
GATGGAAAAC CACTCCTGCC AAAAGTGCCC AAGGAATCGC TTTTACCCAT GAGTGAAGAC 
TTCCTTCTTG ATGCTTTGTC CAAGGACTTT GCTGGTTCCT CAAACACTTC ACCTCTTGAA 
TTTGAAGAGG CTAACCTTCC TGCTGTCATC TCTGAAGTGG TTTCCCAAAC CCCAGCTCCA 
ACCACGCACT CTGCTGGTCT ACCCCCTGAT ACTGTGCAAA GTGACAACAA AGAACTGGAC 
GATGCTTTGG ACCAACTTTC TGACAGTCTG GGACAAAGGC AGCCTGATCC AGATGAGAAC 
AAGCCGATAG AGGATAAAGT CAAGGAAAGA GCTAAAGCTG AACACAGAGA CAAGCTGGGA 
GAAAGAGACG ACACCATCCC CCCTGAATAT AGACACCTCT TGGATAACGA TGAGGGTAAA 
CCAGGAAAGC CACCTGCCAA GAAAACCAAG GACTCAAAGA AGCCTGAAGA TGACAAAGAC 
CCCATCGATG CCCTGTCAGG CGACTTCGAC AGCTGTCCTG CACCCACAGA AGCCTCAGAG 
AACACAGCAG AGGACAAAGC CAAGAGAACT GCTCCCAGCT CCAAAACACC GAAGAATAGG 
GGTAAAGCAA AGGATTCTGC AAAGGCAAAG CAGGAAACTT CCAAGCCCAA AGCTGATGAA 
AAAAATACAA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967672.1
CDS	79..2433
Translation	MSQPGQKPAASPRPRRAAAARDTQEHVSEKTSGSPSKSGEKKGADEKKARSLESSQPSRTHAGGTAPATKASASSASTSKSSSMNPTETKAIPVSKQTGGQHSPNKKRHKKQAAKTEPEKKSQSTKPKSLPKHASDMGSKHVHSEKAASRSSEQLTSEKSTKPKTKAQDSVSAGGESVVAGAAAAAASATAANKQKKEKKSLTSAVPVESKPGKPPGKSDMDAALDDLVDTLGKPEETKEDNVPYTGPEISDPMTSTYIEELGKREVTLPPKYRELLAKKEGITVPPPDSSKPMGPNDAIDALSSDFTCSSPTDAGKKTKEEKSTGEEILKAQPARVTKSAASPQEKKRKVEEQDAMSDQALEALSASLGSRKPEPELDLSSIKEVDEAKAKEEKLKKCGEDEETVPSEYRLKPATDKDGKPLLPEPEEKSKPLSESELIDELSEDFNQPKSKGEQSKPTEKTEESQAAPLTPVSEAVPRTSMCSIQSAPPKPPTLEGMVPDDAVEALAGSLGKKEADPGDGKPVEDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKVPKESLLPMSEDFLLDALSKDFAGSSNTSPLEFEEANLPAVISEVVSQTPAPTTHSAGLPPDTVQSDNKELDDALDQLSDSLGQRQPDPDENKPIEDKVKERAKAEHRDKLGERDDTIPPEYRHLLDNDEGKPGKPPAKKTKDSKKPEDDKDPIDALSGDFDSCPAPTEASENTAEDKAKRTAPSSKTPKNRGKAKDSAKAKQETSKPKADEKNTS

Target ORF information:

RefSeq Version	XM_014112197.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calpastatin (CAST), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014112197.2

ORF Insert Sequence:

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTATTCCAG TCAGCAAACA GACGGGAGGA 
CAGCATTCTC CTAACAAGAA AAGACACAAA AAACAGGCTG CAAAAACAGA ACCTGAAAAG 
AAGTCACAAT CAACTAAGCC AAAAAGCCTA CCCAAGCATG CATCAGATAT GGGAAGCAAG 
CATGTTCATA GTGAAAAAGC AGCTTCTAGA TCAAGTGAGC AGCTGACATC AGAGAAATCA 
ACAAAACCAA AGACTAAAGC ACAGGATTCC GTTTCTGCCG GTGGGGAGAG TGTTGTTGCA 
GGTGCAGCTG CAGCTGCAGC ATCTGCCACA GCAGCTAACA AGCAGAAAAA AGAAAAGAAA 
TCACTAACAT CGGCTGTACC AGTTGAATCC AAACCGGGTA AACCACCTGG AAAGTCAGAC 
ATGGATGCTG CTTTGGATGA CTTAGTAGAC ACTCTAGGAA AACCTGAAGA AACTAAAGAA 
GATAATGTAC CATATACTGG ACCTGAAATT TCAGATCCGA TGACTTCCAC CTACATAGAG 
GAATTAGGTA AAAGAGAAGT CACACTTCCT CCAAAGTACA GGGAACTTTT GGCAAAAAAA 
GAAGGTATCA CAGTGCCTCC TCCAGACTCT TCGAAACCCA TGGGGCCCAA TGATGCCATA 
GATGCTTTGT CATCTGACTT CACCTGCAGT TCACCTACCG ATGCTGGAAA GAAAACTAAG 
GAAGAGAAAT CTACAGGAGA AGAGATTTTA AAAGCCCAGC CAGCCAGGGT AACCAAAAGT 
GCTGCTTCAC CCCAAGAGAA AAAAAGAAAG GTGGAGGAGC AGGACGCAAT GAGTGATCAA 
GCACTGGAGG CTCTTTCAGC TTCTTTGGGC AGCCGGAAGC CAGAACCCGA GCTGGACCTC 
AGTTCCATTA AGGAAGTCGA CGAGGCAAAA GCCAAAGAAG AGAAACTAAA GAAGTGTGGT 
GAAGATGAGG AAACTGTCCC ATCAGAGTAC AGATTAAAAC CAGCTACGGA TAAAGATGGA 
AAACCTCTAT TGCCAGAGCC TGAAGAAAAA TCCAAGCCCC TGAGTGAATC AGAACTCATT 
GATGAGCTTT CAGAAGATTT TAACCAGCCT AAAAGTAAAG GAGAACAGTC TAAGCCAACT 
GAAAAAACAG AAGAATCTCA GGCTGCCCCC CTAACTCCTG TGAGCGAGGC TGTGCCTCGG 
ACCTCCATGT GTAGCATACA GTCGGCACCG CCCAAGCCAC CTACCTTGGA AGGCATGGTG 
CCAGATGATG CTGTAGAAGC CTTGGCTGGT AGCCTGGGGA AGAAGGAAGC AGATCCAGGA 
GATGGAAAGC CTGTGGAAGA TAAAGTCAAG GAGAAAGCCA AGGAAGAGGA TCGTGAAAAA 
CTTGGTGAAA AAGAAGAAAC AATTCCTCCT GATTATAGAT TAGAAGAGGT CAAGGATAAA 
GATGGAAAAC CACTCCTGCC AAAAGTGCCC AAGGAATCGC TTTTACCCAT GAGTGAAGAC 
TTCCTTCTTG ATGCTTTGTC CAAGGACTTT GCTGGTTCCT CAAACACTTC ACCTCTTGAA 
TTTGAAGAGG CTAACCTTCC TGCTGTCATC TCTGAAGTGG TTTCCCAAAC CCCAGCTCCA 
ACCACGCACT CTGCTGGTCT ACCCCCTGAT ACTGTGCAAA GTGACAACAA AGAACTGGAC 
GATGCTTTGG ACCAACTTTC TGACAGTCTG GGACAAAGGC AGCCTGATCC AGATGAGAAC 
AAGCCGATAG AGGATAAAGT CAAGGAAAGA GCTAAAGCTG AACACAGAGA CAAGCTGGGA 
GAAAGAGACG ACACCATCCC CCCTGAATAT AGACACCTCT TGGATAACGA TGAGGGTAAA 
CCAGGAAAGC CACCTGCCAA GAAAACCAAG GACTCAAAGA AGCCTGAAGA TGACAAAGAC 
CCCATCGATG CCCTGTCAGG CGACTTCGAC AGCTGTCCTG CACCCACAGA AGCCTCAGAG 
AACACAGCAG AGGACAAAGC CAAGAGAACT GCTCCCAGCT CCAAAACACC GAAGAATAGG 
GGTAAAGCAA AGGATTCTGC AAAGGCAAAG CAGGAAACTT CCAAGCCCAA AGCTGATGAA 
AAAAATACAA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_014112198.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013967673.1 Canis lupus familiaris calpastatin (CAST), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OCe35585
Clone ID Related Accession (Same CDS sequence)	XM_014112198.2 , XM_014112198.1
Accession Version	XM_014112198.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	calpastatin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014112198.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGCAAAAA CAGAACCTGA AAAGAAGTCA 
CAATCAACTA AGCCATCTGT GGTCCATGAA AAAAAAACCC AAGAAGTAAA GCCAAAGGAA 
CACAAAGAGC CAAAAAGCCT ACCCAAGCAT GCATCAGATA TGGGAAGCAA GCATGTTCAT 
AGTGAAAAAG CAGCTTCTAG ATCAAGTGAG CAGCTGACAT CAGAGAAATC AACAAAACCA 
AAGACTAAAG CACAGGATTC CGTTTCTGCC GGTGGGGAGA GTGTTGTTGC AGGTGCAGCT 
GCAGCTGCAG CATCTGCCAC AGCAGCTAAC AAGCAGAAAA AAGAAAAGAA ATCACTAACA 
TCGGCTGTAC CAGTTGAATC CAAACCGGGT AAACCACCTG GAAAGTCAGA CATGGATGCT 
GCTTTGGATG ACTTAGTAGA CACTCTAGGA AAACCTGAAG AAACTAAAGA AGATAATGTA 
CCATATACTG GACCTGAAAT TTCAGATCCG ATGACTTCCA CCTACATAGA GGAATTAGGT 
AAAAGAGAAG TCACACTTCC TCCAAAGTAC AGGGAACTTT TGGCAAAAAA AGAAGGTATC 
ACAGTGCCTC CTCCAGACTC TTCGAAACCC ATGGGGCCCA ATGATGCCAT AGATGCTTTG 
TCATCTGACT TCACCTGCAG TTCACCTACC GATGCTGGAA AGAAAACTAA GGAAGAGAAA 
TCTACAGGAG AAGAGATTTT AAAAGCCCAG CCAGCCAGGG TAACCAAAAG TGCTGCTTCA 
CCCCAAGAGA AAAAAAGAAA GGTGGAGGAG CAGGACGCAA TGAGTGATCA AGCACTGGAG 
GCTCTTTCAG CTTCTTTGGG CAGCCGGAAG CCAGAACCCG AGCTGGACCT CAGTTCCATT 
AAGGAAGTCG ACGAGGCAAA AGCCAAAGAA GAGAAACTAA AGAAGTGTGG TGAAGATGAG 
GAAACTGTCC CATCAGAGTA CAGATTAAAA CCAGCTACGG ATAAAGATGG AAAACCTCTA 
TTGCCAGAGC CTGAAGAAAA ATCCAAGCCC CTGAGTGAAT CAGAACTCAT TGATGAGCTT 
TCAGAAGATT TTAACCAGCC TAAAAGTAAA GGAGAACAGT CTAAGCCAAC TGAAAAAACA 
GAAGAATCTC AGGCTGCCCC CCTAACTCCT GTGAGCGAGG CTGTGCCTCG GACCTCCATG 
TGTAGCATAC AGTCGGCACC GCCCAAGCCA CCTACCTTGG AAGGCATGGT GCCAGATGAT 
GCTGTAGAAG CCTTGGCTGG TAGCCTGGGG AAGAAGGAAG CAGATCCAGG AGATGGAAAG 
CCTGTGGAAG ATAAAGTCAA GGAGAAAGCC AAGGAAGAGG ATCGTGAAAA ACTTGGTGAA 
AAAGAAGAAA CAATTCCTCC TGATTATAGA TTAGAAGAGG TCAAGGATAA AGATGGAAAA 
CCACTCCTGC CAAAAGTGCC CAAGGAATCG CTTTTACCCA TGAGTGAAGA CTTCCTTCTT 
GATGCTTTGT CCAAGGACTT TGCTGGTTCC TCAAACACTT CACCTCTTGA ATTTGAAGAG 
GCTAACCTTC CTGCTGTCAT CTCTGAAGTG GTTTCCCAAA CCCCAGCTCC AACCACGCAC 
TCTGCTGGTC TACCCCCTGA TACTGTGCAA AGTGACAACA AAGAACTGGA CGATGCTTTG 
GACCAACTTT CTGACAGTCT GGGACAAAGG CAGCCTGATC CAGATGAGAA CAAGCCGATA 
GAGGATAAAG TCAAGGAAAG AGCTAAAGCT GAACACAGAG ACAAGCTGGG AGAAAGAGAC 
GACACCATCC CCCCTGAATA TAGACACCTC TTGGATAACG ATGAGGGTAA ACCAGGAAAG 
CCACCTGCCA AGAAAACCAA GGACTCAAAG AAGCCTGAAG ATGACAAAGA CCCCATCGAT 
GCCCTGTCAG GCGACTTCGA CAGCTGTCCT GCACCCACAG AAGCCTCAGA GAACACAGCA 
GAGGACAAAG CCAAGAGAAC TGCTCCCAGC TCCAAAACAC CGAAGAATAG GGGTAAAGCA 
AAGGATTCTG CAAAGGCAAA GCAGGAAACT TCCAAGCCCA AAGCTGATGA AAAAAATACA 
AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967673.1
CDS	79..2424
Translation	MSQPGQKPAASPRPRRAAAARDTQEHVSEKTSGSPSKSGEKKGADEKKARSLESSQPSRTHAGGTAPATKASASSASTSKSSSMNPTETKAAKTEPEKKSQSTKPSVVHEKKTQEVKPKEHKEPKSLPKHASDMGSKHVHSEKAASRSSEQLTSEKSTKPKTKAQDSVSAGGESVVAGAAAAAASATAANKQKKEKKSLTSAVPVESKPGKPPGKSDMDAALDDLVDTLGKPEETKEDNVPYTGPEISDPMTSTYIEELGKREVTLPPKYRELLAKKEGITVPPPDSSKPMGPNDAIDALSSDFTCSSPTDAGKKTKEEKSTGEEILKAQPARVTKSAASPQEKKRKVEEQDAMSDQALEALSASLGSRKPEPELDLSSIKEVDEAKAKEEKLKKCGEDEETVPSEYRLKPATDKDGKPLLPEPEEKSKPLSESELIDELSEDFNQPKSKGEQSKPTEKTEESQAAPLTPVSEAVPRTSMCSIQSAPPKPPTLEGMVPDDAVEALAGSLGKKEADPGDGKPVEDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKVPKESLLPMSEDFLLDALSKDFAGSSNTSPLEFEEANLPAVISEVVSQTPAPTTHSAGLPPDTVQSDNKELDDALDQLSDSLGQRQPDPDENKPIEDKVKERAKAEHRDKLGERDDTIPPEYRHLLDNDEGKPGKPPAKKTKDSKKPEDDKDPIDALSGDFDSCPAPTEASENTAEDKAKRTAPSSKTPKNRGKAKDSAKAKQETSKPKADEKNTS

Target ORF information:

RefSeq Version	XM_014112198.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calpastatin (CAST), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014112198.2

ORF Insert Sequence:

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGCAAAAA CAGAACCTGA AAAGAAGTCA 
CAATCAACTA AGCCATCTGT GGTCCATGAA AAAAAAACCC AAGAAGTAAA GCCAAAGGAA 
CACAAAGAGC CAAAAAGCCT ACCCAAGCAT GCATCAGATA TGGGAAGCAA GCATGTTCAT 
AGTGAAAAAG CAGCTTCTAG ATCAAGTGAG CAGCTGACAT CAGAGAAATC AACAAAACCA 
AAGACTAAAG CACAGGATTC CGTTTCTGCC GGTGGGGAGA GTGTTGTTGC AGGTGCAGCT 
GCAGCTGCAG CATCTGCCAC AGCAGCTAAC AAGCAGAAAA AAGAAAAGAA ATCACTAACA 
TCGGCTGTAC CAGTTGAATC CAAACCGGGT AAACCACCTG GAAAGTCAGA CATGGATGCT 
GCTTTGGATG ACTTAGTAGA CACTCTAGGA AAACCTGAAG AAACTAAAGA AGATAATGTA 
CCATATACTG GACCTGAAAT TTCAGATCCG ATGACTTCCA CCTACATAGA GGAATTAGGT 
AAAAGAGAAG TCACACTTCC TCCAAAGTAC AGGGAACTTT TGGCAAAAAA AGAAGGTATC 
ACAGTGCCTC CTCCAGACTC TTCGAAACCC ATGGGGCCCA ATGATGCCAT AGATGCTTTG 
TCATCTGACT TCACCTGCAG TTCACCTACC GATGCTGGAA AGAAAACTAA GGAAGAGAAA 
TCTACAGGAG AAGAGATTTT AAAAGCCCAG CCAGCCAGGG TAACCAAAAG TGCTGCTTCA 
CCCCAAGAGA AAAAAAGAAA GGTGGAGGAG CAGGACGCAA TGAGTGATCA AGCACTGGAG 
GCTCTTTCAG CTTCTTTGGG CAGCCGGAAG CCAGAACCCG AGCTGGACCT CAGTTCCATT 
AAGGAAGTCG ACGAGGCAAA AGCCAAAGAA GAGAAACTAA AGAAGTGTGG TGAAGATGAG 
GAAACTGTCC CATCAGAGTA CAGATTAAAA CCAGCTACGG ATAAAGATGG AAAACCTCTA 
TTGCCAGAGC CTGAAGAAAA ATCCAAGCCC CTGAGTGAAT CAGAACTCAT TGATGAGCTT 
TCAGAAGATT TTAACCAGCC TAAAAGTAAA GGAGAACAGT CTAAGCCAAC TGAAAAAACA 
GAAGAATCTC AGGCTGCCCC CCTAACTCCT GTGAGCGAGG CTGTGCCTCG GACCTCCATG 
TGTAGCATAC AGTCGGCACC GCCCAAGCCA CCTACCTTGG AAGGCATGGT GCCAGATGAT 
GCTGTAGAAG CCTTGGCTGG TAGCCTGGGG AAGAAGGAAG CAGATCCAGG AGATGGAAAG 
CCTGTGGAAG ATAAAGTCAA GGAGAAAGCC AAGGAAGAGG ATCGTGAAAA ACTTGGTGAA 
AAAGAAGAAA CAATTCCTCC TGATTATAGA TTAGAAGAGG TCAAGGATAA AGATGGAAAA 
CCACTCCTGC CAAAAGTGCC CAAGGAATCG CTTTTACCCA TGAGTGAAGA CTTCCTTCTT 
GATGCTTTGT CCAAGGACTT TGCTGGTTCC TCAAACACTT CACCTCTTGA ATTTGAAGAG 
GCTAACCTTC CTGCTGTCAT CTCTGAAGTG GTTTCCCAAA CCCCAGCTCC AACCACGCAC 
TCTGCTGGTC TACCCCCTGA TACTGTGCAA AGTGACAACA AAGAACTGGA CGATGCTTTG 
GACCAACTTT CTGACAGTCT GGGACAAAGG CAGCCTGATC CAGATGAGAA CAAGCCGATA 
GAGGATAAAG TCAAGGAAAG AGCTAAAGCT GAACACAGAG ACAAGCTGGG AGAAAGAGAC 
GACACCATCC CCCCTGAATA TAGACACCTC TTGGATAACG ATGAGGGTAA ACCAGGAAAG 
CCACCTGCCA AGAAAACCAA GGACTCAAAG AAGCCTGAAG ATGACAAAGA CCCCATCGAT 
GCCCTGTCAG GCGACTTCGA CAGCTGTCCT GCACCCACAG AAGCCTCAGA GAACACAGCA 
GAGGACAAAG CCAAGAGAAC TGCTCCCAGC TCCAAAACAC CGAAGAATAG GGGTAAAGCA 
AAGGATTCTG CAAAGGCAAA GCAGGAAACT TCCAAGCCCA AAGCTGATGA AAAAAATACA 
AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_014112198.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013967673.1 Canis lupus familiaris calpastatin (CAST), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OCe35585
Clone ID Related Accession (Same CDS sequence)	XM_014112198.2 , XM_014112198.1
Accession Version	XM_014112198.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	calpastatin isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726052.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGCAAAAA CAGAACCTGA AAAGAAGTCA 
CAATCAACTA AGCCATCTGT GGTCCATGAA AAAAAAACCC AAGAAGTAAA GCCAAAGGAA 
CACAAAGAGC CAAAAAGCCT ACCCAAGCAT GCATCAGATA TGGGAAGCAA GCATGTTCAT 
AGTGAAAAAG CAGCTTCTAG ATCAAGTGAG CAGCTGACAT CAGAGAAATC AACAAAACCA 
AAGACTAAAG CACAGGATTC CGTTTCTGCC GGTGGGGAGA GTGTTGTTGC AGGTGCAGCT 
GCAGCTGCAG CATCTGCCAC AGCAGCTAAC AAGCAGAAAA AAGAAAAGAA ATCACTAACA 
TCGGCTGTAC CAGTTGAATC CAAACCGGGT AAACCACCTG GAAAGTCAGA CATGGATGCT 
GCTTTGGATG ACTTAGTAGA CACTCTAGGA AAACCTGAAG AAACTAAAGA AGATAATGTA 
CCATATACTG GACCTGAAAT TTCAGATCCG ATGACTTCCA CCTACATAGA GGAATTAGGT 
AAAAGAGAAG TCACACTTCC TCCAAAGTAC AGGGAACTTT TGGCAAAAAA AGAAGGTATC 
ACAGTGCCTC CTCCAGACTC TTCGAAACCC ATGGGGCCCA ATGATGCCAT AGATGCTTTG 
TCATCTGACT TCACCTGCAG TTCACCTACC GATGCTGGAA AGAAAACTAA GGAAGAGAAA 
TCTACAGGAG AAGAGATTTT AAAAGCCCAG CCAGCCAGGG TAACCAAAAG TGCTGCTTCA 
CCCCAAGAGA AAAAAAGAAA GGTGGAGGAG CAGGACGCAA TGAGTGATCA AGCACTGGAG 
GCTCTTTCAG CTTCTTTGGG CAGCCGGAAG CCAGAACCCG AGCTGGACCT CAGTTCCATT 
AAGGAAGTCG ACGAGGCAAA AGCCAAAGAA GAGAAACTAA AGAAGTGTGG TGAAGATGAG 
GAAACTGTCC CATCAGAGTA CAGATTAAAA CCAGCTACGG ATAAAGATGG AAAACCTCTA 
TTGCCAGAGC CTGAAGAAAA ATCCAAGCCC CTGAGTGAAT CAGAACTCAT TGATGAGCTT 
TCAGAAGATT TTAACCAGCC TAAAAGTAAA GGAGAACAGT CTAAGCCAAC TGAAAAAACA 
GAAGAATCTC AGGCTGCCCC CCTAACTCCT GTGAGCGAGG CTGTGCCTCG GACCTCCATG 
TGTAGCATAC AGTCGGCACC GCCCAAGCCA CCTACCTTGG AAGGCATGGT GCCAGATGAT 
GCTGTAGAAG CCTTGGCTGG TAGCCTGGGG AAGAAGGAAG CAGATCCAGG AGATGGAAAG 
CCTGTGGAAG ATAAAGTCAA GGAGAAAGCC AAGGAAGAGG ATCGTGAAAA ACTTGGTGAA 
AAAGAAGAAA CAATTCCTCC TGATTATAGA TTAGAAGAGG TCAAGGATAA AGATGGAAAA 
CCACTCCTGC CAAAAGTGCC CAAGGAATCG CTTTTACCCA TGAGTGAAGA CTTCCTTCTT 
GATGCTTTGT CCAAGGACTT TGCTGGTTCC TCAAACACTT CACCTCTTGA ATTTGAAGAG 
GCTAACCTTC CTGCTGTCAT CTCTGAAGTG GTTTCCCAAA CCCCAGCTCC AACCACGCAC 
TCTGCTGGTC TACCCCCTGA TACTGTGCAA AGTGACAACA AAGAACTGGA CGATGCTTTG 
GACCAACTTT CTGACAGTCT GGGACAAAGG CAGCCTGATC CAGATGAGAA CAAGCCGATA 
GAGGATAAAG TCAAGGAAAG AGCTAAAGCT GAACACAGAG ACAAGCTGGG AGAAAGAGAC 
GACACCATCC CCCCTGAATA TAGACACCTC TTGGATAACG ATGAGGGTAA ACCAGGAAAG 
CCACCTGCCA AGAAAACCAA GGACTCAAAG AAGCCTGAAG ATGACAAAGA CCCCATCGAT 
GCCCTGTCAG GCGACTTCGA CAGCTGTCCT GCACCCACAG AAGCCTCAGA GAACACAGCA 
GAGGACAAAG CCAAGAGAAC TGCTCCCAGC TCCAAAACAC CGAAGAATAG GGGTAAAGCA 
AAGGATTCTG CAAAGGCAAA GCAGGAAACT TCCAAGCCCA AAGCTGATGA AAAAAATACA 
AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967673.1
CDS	102..2447
Translation	MSQPGQKPAASPRPRRAAAARDTQEHVSEKTSGSPSKSGEKKGADEKKARSLESSQPSRTHAGGTAPATKASASSASTSKSSSMNPTETKAAKTEPEKKSQSTKPSVVHEKKTQEVKPKEHKEPKSLPKHASDMGSKHVHSEKAASRSSEQLTSEKSTKPKTKAQDSVSAGGESVVAGAAAAAASATAANKQKKEKKSLTSAVPVESKPGKPPGKSDMDAALDDLVDTLGKPEETKEDNVPYTGPEISDPMTSTYIEELGKREVTLPPKYRELLAKKEGITVPPPDSSKPMGPNDAIDALSSDFTCSSPTDAGKKTKEEKSTGEEILKAQPARVTKSAASPQEKKRKVEEQDAMSDQALEALSASLGSRKPEPELDLSSIKEVDEAKAKEEKLKKCGEDEETVPSEYRLKPATDKDGKPLLPEPEEKSKPLSESELIDELSEDFNQPKSKGEQSKPTEKTEESQAAPLTPVSEAVPRTSMCSIQSAPPKPPTLEGMVPDDAVEALAGSLGKKEADPGDGKPVEDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKVPKESLLPMSEDFLLDALSKDFAGSSNTSPLEFEEANLPAVISEVVSQTPAPTTHSAGLPPDTVQSDNKELDDALDQLSDSLGQRQPDPDENKPIEDKVKERAKAEHRDKLGERDDTIPPEYRHLLDNDEGKPGKPPAKKTKDSKKPEDDKDPIDALSGDFDSCPAPTEASENTAEDKAKRTAPSSKTPKNRGKAKDSAKAKQETSKPKADEKNTS

Target ORF information:

RefSeq Version	XM_014112198.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calpastatin (CAST), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014112198.1

ORF Insert Sequence:

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGCAAAAA CAGAACCTGA AAAGAAGTCA 
CAATCAACTA AGCCATCTGT GGTCCATGAA AAAAAAACCC AAGAAGTAAA GCCAAAGGAA 
CACAAAGAGC CAAAAAGCCT ACCCAAGCAT GCATCAGATA TGGGAAGCAA GCATGTTCAT 
AGTGAAAAAG CAGCTTCTAG ATCAAGTGAG CAGCTGACAT CAGAGAAATC AACAAAACCA 
AAGACTAAAG CACAGGATTC CGTTTCTGCC GGTGGGGAGA GTGTTGTTGC AGGTGCAGCT 
GCAGCTGCAG CATCTGCCAC AGCAGCTAAC AAGCAGAAAA AAGAAAAGAA ATCACTAACA 
TCGGCTGTAC CAGTTGAATC CAAACCGGGT AAACCACCTG GAAAGTCAGA CATGGATGCT 
GCTTTGGATG ACTTAGTAGA CACTCTAGGA AAACCTGAAG AAACTAAAGA AGATAATGTA 
CCATATACTG GACCTGAAAT TTCAGATCCG ATGACTTCCA CCTACATAGA GGAATTAGGT 
AAAAGAGAAG TCACACTTCC TCCAAAGTAC AGGGAACTTT TGGCAAAAAA AGAAGGTATC 
ACAGTGCCTC CTCCAGACTC TTCGAAACCC ATGGGGCCCA ATGATGCCAT AGATGCTTTG 
TCATCTGACT TCACCTGCAG TTCACCTACC GATGCTGGAA AGAAAACTAA GGAAGAGAAA 
TCTACAGGAG AAGAGATTTT AAAAGCCCAG CCAGCCAGGG TAACCAAAAG TGCTGCTTCA 
CCCCAAGAGA AAAAAAGAAA GGTGGAGGAG CAGGACGCAA TGAGTGATCA AGCACTGGAG 
GCTCTTTCAG CTTCTTTGGG CAGCCGGAAG CCAGAACCCG AGCTGGACCT CAGTTCCATT 
AAGGAAGTCG ACGAGGCAAA AGCCAAAGAA GAGAAACTAA AGAAGTGTGG TGAAGATGAG 
GAAACTGTCC CATCAGAGTA CAGATTAAAA CCAGCTACGG ATAAAGATGG AAAACCTCTA 
TTGCCAGAGC CTGAAGAAAA ATCCAAGCCC CTGAGTGAAT CAGAACTCAT TGATGAGCTT 
TCAGAAGATT TTAACCAGCC TAAAAGTAAA GGAGAACAGT CTAAGCCAAC TGAAAAAACA 
GAAGAATCTC AGGCTGCCCC CCTAACTCCT GTGAGCGAGG CTGTGCCTCG GACCTCCATG 
TGTAGCATAC AGTCGGCACC GCCCAAGCCA CCTACCTTGG AAGGCATGGT GCCAGATGAT 
GCTGTAGAAG CCTTGGCTGG TAGCCTGGGG AAGAAGGAAG CAGATCCAGG AGATGGAAAG 
CCTGTGGAAG ATAAAGTCAA GGAGAAAGCC AAGGAAGAGG ATCGTGAAAA ACTTGGTGAA 
AAAGAAGAAA CAATTCCTCC TGATTATAGA TTAGAAGAGG TCAAGGATAA AGATGGAAAA 
CCACTCCTGC CAAAAGTGCC CAAGGAATCG CTTTTACCCA TGAGTGAAGA CTTCCTTCTT 
GATGCTTTGT CCAAGGACTT TGCTGGTTCC TCAAACACTT CACCTCTTGA ATTTGAAGAG 
GCTAACCTTC CTGCTGTCAT CTCTGAAGTG GTTTCCCAAA CCCCAGCTCC AACCACGCAC 
TCTGCTGGTC TACCCCCTGA TACTGTGCAA AGTGACAACA AAGAACTGGA CGATGCTTTG 
GACCAACTTT CTGACAGTCT GGGACAAAGG CAGCCTGATC CAGATGAGAA CAAGCCGATA 
GAGGATAAAG TCAAGGAAAG AGCTAAAGCT GAACACAGAG ACAAGCTGGG AGAAAGAGAC 
GACACCATCC CCCCTGAATA TAGACACCTC TTGGATAACG ATGAGGGTAA ACCAGGAAAG 
CCACCTGCCA AGAAAACCAA GGACTCAAAG AAGCCTGAAG ATGACAAAGA CCCCATCGAT 
GCCCTGTCAG GCGACTTCGA CAGCTGTCCT GCACCCACAG AAGCCTCAGA GAACACAGCA 
GAGGACAAAG CCAAGAGAAC TGCTCCCAGC TCCAAAACAC CGAAGAATAG GGGTAAAGCA 
AAGGATTCTG CAAAGGCAAA GCAGGAAACT TCCAAGCCCA AAGCTGATGA AAAAAATACA 
AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_014112199.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013967674.1 Canis lupus familiaris calpastatin (CAST), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OCe35586
Clone ID Related Accession (Same CDS sequence)	XM_014112199.1 , XM_014112199.2
Accession Version	XM_014112199.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	calpastatin isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726052.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGCAAAAA CAGAACCTGA AAAGAAGTCA 
CAATCAACTA AGCCATCTGT GGTCCATGAA AAAAAAACCC AAGAAGTAAA GCCAAAGGAA 
CACAAAGAGC CAAAAAGCCT ACCCAAGCAT GCATCAGATA TGGGAAGCAA GCATGTTCAT 
AGTGAAAAAG CAGCTTCTAG ATCAAGTGAG CAGCTGACAT CAGAGAAATC AACAAAACCA 
AAGACTAAAG CACAGGATTC CGTTTCTGCC GGTGGGGAGA GTGTTGTTGC AGGTGCAGCT 
GCAGCTGCAG CATCTGCCAC AGCAGCTAAC AAGAAAAAAG AAAAGAAATC ACTAACATCG 
GCTGTACCAG TTGAATCCAA ACCGGGTAAA CCACCTGGAA AGTCAGACAT GGATGCTGCT 
TTGGATGACT TAGTAGACAC TCTAGGAAAA CCTGAAGAAA CTAAAGAAGA TAATGTACCA 
TATACTGGAC CTGAAATTTC AGATCCGATG ACTTCCACCT ACATAGAGGA ATTAGGTAAA 
AGAGAAGTCA CACTTCCTCC AAAGTACAGG GAACTTTTGG CAAAAAAAGA AGGTATCACA 
GTGCCTCCTC CAGACTCTTC GAAACCCATG GGGCCCAATG ATGCCATAGA TGCTTTGTCA 
TCTGACTTCA CCTGCAGTTC ACCTACCGAT GCTGGAAAGA AAACTAAGGA AGAGAAATCT 
ACAGGAGAAG AGATTTTAAA AGCCCAGCCA GCCAGGGTAA CCAAAAGTGC TGCTTCACCC 
CAAGAGAAAA AAAGAAAGGT GGAGGAGCAG GACGCAATGA GTGATCAAGC ACTGGAGGCT 
CTTTCAGCTT CTTTGGGCAG CCGGAAGCCA GAACCCGAGC TGGACCTCAG TTCCATTAAG 
GAAGTCGACG AGGCAAAAGC CAAAGAAGAG AAACTAAAGA AGTGTGGTGA AGATGAGGAA 
ACTGTCCCAT CAGAGTACAG ATTAAAACCA GCTACGGATA AAGATGGAAA ACCTCTATTG 
CCAGAGCCTG AAGAAAAATC CAAGCCCCTG AGTGAATCAG AACTCATTGA TGAGCTTTCA 
GAAGATTTTA ACCAGCCTAA AAGTAAAGGA GAACAGTCTA AGCCAACTGA AAAAACAGAA 
GAATCTCAGG CTGCCCCCCT AACTCCTGTG AGCGAGGCTG TGCCTCGGAC CTCCATGTGT 
AGCATACAGT CGGCACCGCC CAAGCCACCT ACCTTGGAAG GCATGGTGCC AGATGATGCT 
GTAGAAGCCT TGGCTGGTAG CCTGGGGAAG AAGGAAGCAG ATCCAGGAGA TGGAAAGCCT 
GTGGAAGATA AAGTCAAGGA GAAAGCCAAG GAAGAGGATC GTGAAAAACT TGGTGAAAAA 
GAAGAAACAA TTCCTCCTGA TTATAGATTA GAAGAGGTCA AGGATAAAGA TGGAAAACCA 
CTCCTGCCAA AAGTGCCCAA GGAATCGCTT TTACCCATGA GTGAAGACTT CCTTCTTGAT 
GCTTTGTCCA AGGACTTTGC TGGTTCCTCA AACACTTCAC CTCTTGAATT TGAAGAGGCT 
AACCTTCCTG CTGTCATCTC TGAAGTGGTT TCCCAAACCC CAGCTCCAAC CACGCACTCT 
GCTGGTCTAC CCCCTGATAC TGTGCAAAGT GACAACAAAG AACTGGACGA TGCTTTGGAC 
CAACTTTCTG ACAGTCTGGG ACAAAGGCAG CCTGATCCAG ATGAGAACAA GCCGATAGAG 
GATAAAGTCA AGGAAAGAGC TAAAGCTGAA CACAGAGACA AGCTGGGAGA AAGAGACGAC 
ACCATCCCCC CTGAATATAG ACACCTCTTG GATAACGATG AGGGTAAACC AGGAAAGCCA 
CCTGCCAAGA AAACCAAGGA CTCAAAGAAG CCTGAAGATG ACAAAGACCC CATCGATGCC 
CTGTCAGGCG ACTTCGACAG CTGTCCTGCA CCCACAGAAG CCTCAGAGAA CACAGCAGAG 
GACAAAGCCA AGAGAACTGC TCCCAGCTCC AAAACACCGA AGAATAGGGG TAAAGCAAAG 
GATTCTGCAA AGGCAAAGCA GGAAACTTCC AAGCCCAAAG CTGATGAAAA AAATACAAGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967674.1
CDS	102..2444
Translation	MSQPGQKPAASPRPRRAAAARDTQEHVSEKTSGSPSKSGEKKGADEKKARSLESSQPSRTHAGGTAPATKASASSASTSKSSSMNPTETKAAKTEPEKKSQSTKPSVVHEKKTQEVKPKEHKEPKSLPKHASDMGSKHVHSEKAASRSSEQLTSEKSTKPKTKAQDSVSAGGESVVAGAAAAAASATAANKKKEKKSLTSAVPVESKPGKPPGKSDMDAALDDLVDTLGKPEETKEDNVPYTGPEISDPMTSTYIEELGKREVTLPPKYRELLAKKEGITVPPPDSSKPMGPNDAIDALSSDFTCSSPTDAGKKTKEEKSTGEEILKAQPARVTKSAASPQEKKRKVEEQDAMSDQALEALSASLGSRKPEPELDLSSIKEVDEAKAKEEKLKKCGEDEETVPSEYRLKPATDKDGKPLLPEPEEKSKPLSESELIDELSEDFNQPKSKGEQSKPTEKTEESQAAPLTPVSEAVPRTSMCSIQSAPPKPPTLEGMVPDDAVEALAGSLGKKEADPGDGKPVEDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKVPKESLLPMSEDFLLDALSKDFAGSSNTSPLEFEEANLPAVISEVVSQTPAPTTHSAGLPPDTVQSDNKELDDALDQLSDSLGQRQPDPDENKPIEDKVKERAKAEHRDKLGERDDTIPPEYRHLLDNDEGKPGKPPAKKTKDSKKPEDDKDPIDALSGDFDSCPAPTEASENTAEDKAKRTAPSSKTPKNRGKAKDSAKAKQETSKPKADEKNTS

Target ORF information:

RefSeq Version	XM_014112199.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calpastatin (CAST), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014112199.1

ORF Insert Sequence:

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGCAAAAA CAGAACCTGA AAAGAAGTCA 
CAATCAACTA AGCCATCTGT GGTCCATGAA AAAAAAACCC AAGAAGTAAA GCCAAAGGAA 
CACAAAGAGC CAAAAAGCCT ACCCAAGCAT GCATCAGATA TGGGAAGCAA GCATGTTCAT 
AGTGAAAAAG CAGCTTCTAG ATCAAGTGAG CAGCTGACAT CAGAGAAATC AACAAAACCA 
AAGACTAAAG CACAGGATTC CGTTTCTGCC GGTGGGGAGA GTGTTGTTGC AGGTGCAGCT 
GCAGCTGCAG CATCTGCCAC AGCAGCTAAC AAGAAAAAAG AAAAGAAATC ACTAACATCG 
GCTGTACCAG TTGAATCCAA ACCGGGTAAA CCACCTGGAA AGTCAGACAT GGATGCTGCT 
TTGGATGACT TAGTAGACAC TCTAGGAAAA CCTGAAGAAA CTAAAGAAGA TAATGTACCA 
TATACTGGAC CTGAAATTTC AGATCCGATG ACTTCCACCT ACATAGAGGA ATTAGGTAAA 
AGAGAAGTCA CACTTCCTCC AAAGTACAGG GAACTTTTGG CAAAAAAAGA AGGTATCACA 
GTGCCTCCTC CAGACTCTTC GAAACCCATG GGGCCCAATG ATGCCATAGA TGCTTTGTCA 
TCTGACTTCA CCTGCAGTTC ACCTACCGAT GCTGGAAAGA AAACTAAGGA AGAGAAATCT 
ACAGGAGAAG AGATTTTAAA AGCCCAGCCA GCCAGGGTAA CCAAAAGTGC TGCTTCACCC 
CAAGAGAAAA AAAGAAAGGT GGAGGAGCAG GACGCAATGA GTGATCAAGC ACTGGAGGCT 
CTTTCAGCTT CTTTGGGCAG CCGGAAGCCA GAACCCGAGC TGGACCTCAG TTCCATTAAG 
GAAGTCGACG AGGCAAAAGC CAAAGAAGAG AAACTAAAGA AGTGTGGTGA AGATGAGGAA 
ACTGTCCCAT CAGAGTACAG ATTAAAACCA GCTACGGATA AAGATGGAAA ACCTCTATTG 
CCAGAGCCTG AAGAAAAATC CAAGCCCCTG AGTGAATCAG AACTCATTGA TGAGCTTTCA 
GAAGATTTTA ACCAGCCTAA AAGTAAAGGA GAACAGTCTA AGCCAACTGA AAAAACAGAA 
GAATCTCAGG CTGCCCCCCT AACTCCTGTG AGCGAGGCTG TGCCTCGGAC CTCCATGTGT 
AGCATACAGT CGGCACCGCC CAAGCCACCT ACCTTGGAAG GCATGGTGCC AGATGATGCT 
GTAGAAGCCT TGGCTGGTAG CCTGGGGAAG AAGGAAGCAG ATCCAGGAGA TGGAAAGCCT 
GTGGAAGATA AAGTCAAGGA GAAAGCCAAG GAAGAGGATC GTGAAAAACT TGGTGAAAAA 
GAAGAAACAA TTCCTCCTGA TTATAGATTA GAAGAGGTCA AGGATAAAGA TGGAAAACCA 
CTCCTGCCAA AAGTGCCCAA GGAATCGCTT TTACCCATGA GTGAAGACTT CCTTCTTGAT 
GCTTTGTCCA AGGACTTTGC TGGTTCCTCA AACACTTCAC CTCTTGAATT TGAAGAGGCT 
AACCTTCCTG CTGTCATCTC TGAAGTGGTT TCCCAAACCC CAGCTCCAAC CACGCACTCT 
GCTGGTCTAC CCCCTGATAC TGTGCAAAGT GACAACAAAG AACTGGACGA TGCTTTGGAC 
CAACTTTCTG ACAGTCTGGG ACAAAGGCAG CCTGATCCAG ATGAGAACAA GCCGATAGAG 
GATAAAGTCA AGGAAAGAGC TAAAGCTGAA CACAGAGACA AGCTGGGAGA AAGAGACGAC 
ACCATCCCCC CTGAATATAG ACACCTCTTG GATAACGATG AGGGTAAACC AGGAAAGCCA 
CCTGCCAAGA AAACCAAGGA CTCAAAGAAG CCTGAAGATG ACAAAGACCC CATCGATGCC 
CTGTCAGGCG ACTTCGACAG CTGTCCTGCA CCCACAGAAG CCTCAGAGAA CACAGCAGAG 
GACAAAGCCA AGAGAACTGC TCCCAGCTCC AAAACACCGA AGAATAGGGG TAAAGCAAAG 
GATTCTGCAA AGGCAAAGCA GGAAACTTCC AAGCCCAAAG CTGATGAAAA AAATACAAGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_014112199.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013967674.1 Canis lupus familiaris calpastatin (CAST), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OCe35586
Clone ID Related Accession (Same CDS sequence)	XM_014112199.1 , XM_014112199.2
Accession Version	XM_014112199.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	calpastatin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014112199.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGCAAAAA CAGAACCTGA AAAGAAGTCA 
CAATCAACTA AGCCATCTGT GGTCCATGAA AAAAAAACCC AAGAAGTAAA GCCAAAGGAA 
CACAAAGAGC CAAAAAGCCT ACCCAAGCAT GCATCAGATA TGGGAAGCAA GCATGTTCAT 
AGTGAAAAAG CAGCTTCTAG ATCAAGTGAG CAGCTGACAT CAGAGAAATC AACAAAACCA 
AAGACTAAAG CACAGGATTC CGTTTCTGCC GGTGGGGAGA GTGTTGTTGC AGGTGCAGCT 
GCAGCTGCAG CATCTGCCAC AGCAGCTAAC AAGAAAAAAG AAAAGAAATC ACTAACATCG 
GCTGTACCAG TTGAATCCAA ACCGGGTAAA CCACCTGGAA AGTCAGACAT GGATGCTGCT 
TTGGATGACT TAGTAGACAC TCTAGGAAAA CCTGAAGAAA CTAAAGAAGA TAATGTACCA 
TATACTGGAC CTGAAATTTC AGATCCGATG ACTTCCACCT ACATAGAGGA ATTAGGTAAA 
AGAGAAGTCA CACTTCCTCC AAAGTACAGG GAACTTTTGG CAAAAAAAGA AGGTATCACA 
GTGCCTCCTC CAGACTCTTC GAAACCCATG GGGCCCAATG ATGCCATAGA TGCTTTGTCA 
TCTGACTTCA CCTGCAGTTC ACCTACCGAT GCTGGAAAGA AAACTAAGGA AGAGAAATCT 
ACAGGAGAAG AGATTTTAAA AGCCCAGCCA GCCAGGGTAA CCAAAAGTGC TGCTTCACCC 
CAAGAGAAAA AAAGAAAGGT GGAGGAGCAG GACGCAATGA GTGATCAAGC ACTGGAGGCT 
CTTTCAGCTT CTTTGGGCAG CCGGAAGCCA GAACCCGAGC TGGACCTCAG TTCCATTAAG 
GAAGTCGACG AGGCAAAAGC CAAAGAAGAG AAACTAAAGA AGTGTGGTGA AGATGAGGAA 
ACTGTCCCAT CAGAGTACAG ATTAAAACCA GCTACGGATA AAGATGGAAA ACCTCTATTG 
CCAGAGCCTG AAGAAAAATC CAAGCCCCTG AGTGAATCAG AACTCATTGA TGAGCTTTCA 
GAAGATTTTA ACCAGCCTAA AAGTAAAGGA GAACAGTCTA AGCCAACTGA AAAAACAGAA 
GAATCTCAGG CTGCCCCCCT AACTCCTGTG AGCGAGGCTG TGCCTCGGAC CTCCATGTGT 
AGCATACAGT CGGCACCGCC CAAGCCACCT ACCTTGGAAG GCATGGTGCC AGATGATGCT 
GTAGAAGCCT TGGCTGGTAG CCTGGGGAAG AAGGAAGCAG ATCCAGGAGA TGGAAAGCCT 
GTGGAAGATA AAGTCAAGGA GAAAGCCAAG GAAGAGGATC GTGAAAAACT TGGTGAAAAA 
GAAGAAACAA TTCCTCCTGA TTATAGATTA GAAGAGGTCA AGGATAAAGA TGGAAAACCA 
CTCCTGCCAA AAGTGCCCAA GGAATCGCTT TTACCCATGA GTGAAGACTT CCTTCTTGAT 
GCTTTGTCCA AGGACTTTGC TGGTTCCTCA AACACTTCAC CTCTTGAATT TGAAGAGGCT 
AACCTTCCTG CTGTCATCTC TGAAGTGGTT TCCCAAACCC CAGCTCCAAC CACGCACTCT 
GCTGGTCTAC CCCCTGATAC TGTGCAAAGT GACAACAAAG AACTGGACGA TGCTTTGGAC 
CAACTTTCTG ACAGTCTGGG ACAAAGGCAG CCTGATCCAG ATGAGAACAA GCCGATAGAG 
GATAAAGTCA AGGAAAGAGC TAAAGCTGAA CACAGAGACA AGCTGGGAGA AAGAGACGAC 
ACCATCCCCC CTGAATATAG ACACCTCTTG GATAACGATG AGGGTAAACC AGGAAAGCCA 
CCTGCCAAGA AAACCAAGGA CTCAAAGAAG CCTGAAGATG ACAAAGACCC CATCGATGCC 
CTGTCAGGCG ACTTCGACAG CTGTCCTGCA CCCACAGAAG CCTCAGAGAA CACAGCAGAG 
GACAAAGCCA AGAGAACTGC TCCCAGCTCC AAAACACCGA AGAATAGGGG TAAAGCAAAG 
GATTCTGCAA AGGCAAAGCA GGAAACTTCC AAGCCCAAAG CTGATGAAAA AAATACAAGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967674.1
CDS	79..2421
Translation	MSQPGQKPAASPRPRRAAAARDTQEHVSEKTSGSPSKSGEKKGADEKKARSLESSQPSRTHAGGTAPATKASASSASTSKSSSMNPTETKAAKTEPEKKSQSTKPSVVHEKKTQEVKPKEHKEPKSLPKHASDMGSKHVHSEKAASRSSEQLTSEKSTKPKTKAQDSVSAGGESVVAGAAAAAASATAANKKKEKKSLTSAVPVESKPGKPPGKSDMDAALDDLVDTLGKPEETKEDNVPYTGPEISDPMTSTYIEELGKREVTLPPKYRELLAKKEGITVPPPDSSKPMGPNDAIDALSSDFTCSSPTDAGKKTKEEKSTGEEILKAQPARVTKSAASPQEKKRKVEEQDAMSDQALEALSASLGSRKPEPELDLSSIKEVDEAKAKEEKLKKCGEDEETVPSEYRLKPATDKDGKPLLPEPEEKSKPLSESELIDELSEDFNQPKSKGEQSKPTEKTEESQAAPLTPVSEAVPRTSMCSIQSAPPKPPTLEGMVPDDAVEALAGSLGKKEADPGDGKPVEDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKVPKESLLPMSEDFLLDALSKDFAGSSNTSPLEFEEANLPAVISEVVSQTPAPTTHSAGLPPDTVQSDNKELDDALDQLSDSLGQRQPDPDENKPIEDKVKERAKAEHRDKLGERDDTIPPEYRHLLDNDEGKPGKPPAKKTKDSKKPEDDKDPIDALSGDFDSCPAPTEASENTAEDKAKRTAPSSKTPKNRGKAKDSAKAKQETSKPKADEKNTS

Target ORF information:

RefSeq Version	XM_014112199.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calpastatin (CAST), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014112199.2

ORF Insert Sequence:

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGCAAAAA CAGAACCTGA AAAGAAGTCA 
CAATCAACTA AGCCATCTGT GGTCCATGAA AAAAAAACCC AAGAAGTAAA GCCAAAGGAA 
CACAAAGAGC CAAAAAGCCT ACCCAAGCAT GCATCAGATA TGGGAAGCAA GCATGTTCAT 
AGTGAAAAAG CAGCTTCTAG ATCAAGTGAG CAGCTGACAT CAGAGAAATC AACAAAACCA 
AAGACTAAAG CACAGGATTC CGTTTCTGCC GGTGGGGAGA GTGTTGTTGC AGGTGCAGCT 
GCAGCTGCAG CATCTGCCAC AGCAGCTAAC AAGAAAAAAG AAAAGAAATC ACTAACATCG 
GCTGTACCAG TTGAATCCAA ACCGGGTAAA CCACCTGGAA AGTCAGACAT GGATGCTGCT 
TTGGATGACT TAGTAGACAC TCTAGGAAAA CCTGAAGAAA CTAAAGAAGA TAATGTACCA 
TATACTGGAC CTGAAATTTC AGATCCGATG ACTTCCACCT ACATAGAGGA ATTAGGTAAA 
AGAGAAGTCA CACTTCCTCC AAAGTACAGG GAACTTTTGG CAAAAAAAGA AGGTATCACA 
GTGCCTCCTC CAGACTCTTC GAAACCCATG GGGCCCAATG ATGCCATAGA TGCTTTGTCA 
TCTGACTTCA CCTGCAGTTC ACCTACCGAT GCTGGAAAGA AAACTAAGGA AGAGAAATCT 
ACAGGAGAAG AGATTTTAAA AGCCCAGCCA GCCAGGGTAA CCAAAAGTGC TGCTTCACCC 
CAAGAGAAAA AAAGAAAGGT GGAGGAGCAG GACGCAATGA GTGATCAAGC ACTGGAGGCT 
CTTTCAGCTT CTTTGGGCAG CCGGAAGCCA GAACCCGAGC TGGACCTCAG TTCCATTAAG 
GAAGTCGACG AGGCAAAAGC CAAAGAAGAG AAACTAAAGA AGTGTGGTGA AGATGAGGAA 
ACTGTCCCAT CAGAGTACAG ATTAAAACCA GCTACGGATA AAGATGGAAA ACCTCTATTG 
CCAGAGCCTG AAGAAAAATC CAAGCCCCTG AGTGAATCAG AACTCATTGA TGAGCTTTCA 
GAAGATTTTA ACCAGCCTAA AAGTAAAGGA GAACAGTCTA AGCCAACTGA AAAAACAGAA 
GAATCTCAGG CTGCCCCCCT AACTCCTGTG AGCGAGGCTG TGCCTCGGAC CTCCATGTGT 
AGCATACAGT CGGCACCGCC CAAGCCACCT ACCTTGGAAG GCATGGTGCC AGATGATGCT 
GTAGAAGCCT TGGCTGGTAG CCTGGGGAAG AAGGAAGCAG ATCCAGGAGA TGGAAAGCCT 
GTGGAAGATA AAGTCAAGGA GAAAGCCAAG GAAGAGGATC GTGAAAAACT TGGTGAAAAA 
GAAGAAACAA TTCCTCCTGA TTATAGATTA GAAGAGGTCA AGGATAAAGA TGGAAAACCA 
CTCCTGCCAA AAGTGCCCAA GGAATCGCTT TTACCCATGA GTGAAGACTT CCTTCTTGAT 
GCTTTGTCCA AGGACTTTGC TGGTTCCTCA AACACTTCAC CTCTTGAATT TGAAGAGGCT 
AACCTTCCTG CTGTCATCTC TGAAGTGGTT TCCCAAACCC CAGCTCCAAC CACGCACTCT 
GCTGGTCTAC CCCCTGATAC TGTGCAAAGT GACAACAAAG AACTGGACGA TGCTTTGGAC 
CAACTTTCTG ACAGTCTGGG ACAAAGGCAG CCTGATCCAG ATGAGAACAA GCCGATAGAG 
GATAAAGTCA AGGAAAGAGC TAAAGCTGAA CACAGAGACA AGCTGGGAGA AAGAGACGAC 
ACCATCCCCC CTGAATATAG ACACCTCTTG GATAACGATG AGGGTAAACC AGGAAAGCCA 
CCTGCCAAGA AAACCAAGGA CTCAAAGAAG CCTGAAGATG ACAAAGACCC CATCGATGCC 
CTGTCAGGCG ACTTCGACAG CTGTCCTGCA CCCACAGAAG CCTCAGAGAA CACAGCAGAG 
GACAAAGCCA AGAGAACTGC TCCCAGCTCC AAAACACCGA AGAATAGGGG TAAAGCAAAG 
GATTCTGCAA AGGCAAAGCA GGAAACTTCC AAGCCCAAAG CTGATGAAAA AAATACAAGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_014112200.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013967675.1 Canis lupus familiaris calpastatin (CAST), transcript variant X9, mRNA.

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CloneID	OCe35587
Clone ID Related Accession (Same CDS sequence)	XM_014112200.1 , XM_014112200.2
Accession Version	XM_014112200.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	calpastatin isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726052.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTATTCCAG TCAGCAAACA GACGGGAGGA 
CAGCATTCTC CTAACAAGAA AAGACACAAA AAACAGGCTG CAAAAACAGA ACCTGAAAAG 
AAGTCACAAT CAACTAAGCC ATCTGTGGTC CATGAAAAAA AAACCCAAGA AGTAAAGCCA 
AAGGAACACA AAGAGCCAAA AAGCCTACCC AAGCATGCAT CAGATATGGG AAGCAAGCAT 
GTTCATAGTG AAAAAGCAGC TTCTAGATCA AGTGAGCAGC TGACATCAGA GAAATCAACA 
AAACCAAAGA CTAAAGCACA GGATTCCGTT TCTGCCGGTG GGGAGAGTGT TGTTGCAGGT 
GCAGCTGCAG CTGCAGCATC TGCCACAGCA GCTAACAAGT CAGACATGGA TGCTGCTTTG 
GATGACTTAG TAGACACTCT AGGAAAACCT GAAGAAACTA AAGAAGATAA TGTACCATAT 
ACTGGACCTG AAATTTCAGA TCCGATGACT TCCACCTACA TAGAGGAATT AGGTAAAAGA 
GAAGTCACAC TTCCTCCAAA GTACAGGGAA CTTTTGGCAA AAAAAGAAGG TATCACAGTG 
CCTCCTCCAG ACTCTTCGAA ACCCATGGGG CCCAATGATG CCATAGATGC TTTGTCATCT 
GACTTCACCT GCAGTTCACC TACCGATGCT GGAAAGAAAA CTAAGGAAGA GAAATCTACA 
GGAGAAGAGA TTTTAAAAGC CCAGCCAGCC AGGGTAACCA AAAGTGCTGC TTCACCCCAA 
GAGAAAAAAA GAAAGGTGGA GGAGCAGGAC GCAATGAGTG ATCAAGCACT GGAGGCTCTT 
TCAGCTTCTT TGGGCAGCCG GAAGCCAGAA CCCGAGCTGG ACCTCAGTTC CATTAAGGAA 
GTCGACGAGG CAAAAGCCAA AGAAGAGAAA CTAAAGAAGT GTGGTGAAGA TGAGGAAACT 
GTCCCATCAG AGTACAGATT AAAACCAGCT ACGGATAAAG ATGGAAAACC TCTATTGCCA 
GAGCCTGAAG AAAAATCCAA GCCCCTGAGT GAATCAGAAC TCATTGATGA GCTTTCAGAA 
GATTTTAACC AGCCTAAAAG TAAAGGAGAA CAGTCTAAGC CAACTGAAAA AACAGAAGAA 
TCTCAGGCTG CCCCCCTAAC TCCTGTGAGC GAGGCTGTGC CTCGGACCTC CATGTGTAGC 
ATACAGTCGG CACCGCCCAA GCCACCTACC TTGGAAGGCA TGGTGCCAGA TGATGCTGTA 
GAAGCCTTGG CTGGTAGCCT GGGGAAGAAG GAAGCAGATC CAGGAGATGG AAAGCCTGTG 
GAAGATAAAG TCAAGGAGAA AGCCAAGGAA GAGGATCGTG AAAAACTTGG TGAAAAAGAA 
GAAACAATTC CTCCTGATTA TAGATTAGAA GAGGTCAAGG ATAAAGATGG AAAACCACTC 
CTGCCAAAAG TGCCCAAGGA ATCGCTTTTA CCCATGAGTG AAGACTTCCT TCTTGATGCT 
TTGTCCAAGG ACTTTGCTGG TTCCTCAAAC ACTTCACCTC TTGAATTTGA AGAGGCTAAC 
CTTCCTGCTG TCATCTCTGA AGTGGTTTCC CAAACCCCAG CTCCAACCAC GCACTCTGCT 
GGTCTACCCC CTGATACTGT GCAAAGTGAC AACAAAGAAC TGGACGATGC TTTGGACCAA 
CTTTCTGACA GTCTGGGACA AAGGCAGCCT GATCCAGATG AGAACAAGCC GATAGAGGAT 
AAAGTCAAGG AAAGAGCTAA AGCTGAACAC AGAGACAAGC TGGGAGAAAG AGACGACACC 
ATCCCCCCTG AATATAGACA CCTCTTGGAT AACGATGAGG GTAAACCAGG AAAGCCACCT 
GCCAAGAAAA CCAAGGACTC AAAGAAGCCT GAAGATGACA AAGACCCCAT CGATGCCCTG 
TCAGGCGACT TCGACAGCTG TCCTGCACCC ACAGAAGCCT CAGAGAACAC AGCAGAGGAC 
AAAGCCAAGA GAACTGCTCC CAGCTCCAAA ACACCGAAGA ATAGGGGTAA AGCAAAGGAT 
TCTGCAAAGG CAAAGCAGGA AACTTCCAAG CCCAAAGCTG ATGAAAAAAA TACAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967675.1
CDS	102..2441
Translation	MSQPGQKPAASPRPRRAAAARDTQEHVSEKTSGSPSKSGEKKGADEKKARSLESSQPSRTHAGGTAPATKASASSASTSKSSSMNPTETKAIPVSKQTGGQHSPNKKRHKKQAAKTEPEKKSQSTKPSVVHEKKTQEVKPKEHKEPKSLPKHASDMGSKHVHSEKAASRSSEQLTSEKSTKPKTKAQDSVSAGGESVVAGAAAAAASATAANKSDMDAALDDLVDTLGKPEETKEDNVPYTGPEISDPMTSTYIEELGKREVTLPPKYRELLAKKEGITVPPPDSSKPMGPNDAIDALSSDFTCSSPTDAGKKTKEEKSTGEEILKAQPARVTKSAASPQEKKRKVEEQDAMSDQALEALSASLGSRKPEPELDLSSIKEVDEAKAKEEKLKKCGEDEETVPSEYRLKPATDKDGKPLLPEPEEKSKPLSESELIDELSEDFNQPKSKGEQSKPTEKTEESQAAPLTPVSEAVPRTSMCSIQSAPPKPPTLEGMVPDDAVEALAGSLGKKEADPGDGKPVEDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKVPKESLLPMSEDFLLDALSKDFAGSSNTSPLEFEEANLPAVISEVVSQTPAPTTHSAGLPPDTVQSDNKELDDALDQLSDSLGQRQPDPDENKPIEDKVKERAKAEHRDKLGERDDTIPPEYRHLLDNDEGKPGKPPAKKTKDSKKPEDDKDPIDALSGDFDSCPAPTEASENTAEDKAKRTAPSSKTPKNRGKAKDSAKAKQETSKPKADEKNTS

Target ORF information:

RefSeq Version	XM_014112200.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calpastatin (CAST), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014112200.1

ORF Insert Sequence:

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTATTCCAG TCAGCAAACA GACGGGAGGA 
CAGCATTCTC CTAACAAGAA AAGACACAAA AAACAGGCTG CAAAAACAGA ACCTGAAAAG 
AAGTCACAAT CAACTAAGCC ATCTGTGGTC CATGAAAAAA AAACCCAAGA AGTAAAGCCA 
AAGGAACACA AAGAGCCAAA AAGCCTACCC AAGCATGCAT CAGATATGGG AAGCAAGCAT 
GTTCATAGTG AAAAAGCAGC TTCTAGATCA AGTGAGCAGC TGACATCAGA GAAATCAACA 
AAACCAAAGA CTAAAGCACA GGATTCCGTT TCTGCCGGTG GGGAGAGTGT TGTTGCAGGT 
GCAGCTGCAG CTGCAGCATC TGCCACAGCA GCTAACAAGT CAGACATGGA TGCTGCTTTG 
GATGACTTAG TAGACACTCT AGGAAAACCT GAAGAAACTA AAGAAGATAA TGTACCATAT 
ACTGGACCTG AAATTTCAGA TCCGATGACT TCCACCTACA TAGAGGAATT AGGTAAAAGA 
GAAGTCACAC TTCCTCCAAA GTACAGGGAA CTTTTGGCAA AAAAAGAAGG TATCACAGTG 
CCTCCTCCAG ACTCTTCGAA ACCCATGGGG CCCAATGATG CCATAGATGC TTTGTCATCT 
GACTTCACCT GCAGTTCACC TACCGATGCT GGAAAGAAAA CTAAGGAAGA GAAATCTACA 
GGAGAAGAGA TTTTAAAAGC CCAGCCAGCC AGGGTAACCA AAAGTGCTGC TTCACCCCAA 
GAGAAAAAAA GAAAGGTGGA GGAGCAGGAC GCAATGAGTG ATCAAGCACT GGAGGCTCTT 
TCAGCTTCTT TGGGCAGCCG GAAGCCAGAA CCCGAGCTGG ACCTCAGTTC CATTAAGGAA 
GTCGACGAGG CAAAAGCCAA AGAAGAGAAA CTAAAGAAGT GTGGTGAAGA TGAGGAAACT 
GTCCCATCAG AGTACAGATT AAAACCAGCT ACGGATAAAG ATGGAAAACC TCTATTGCCA 
GAGCCTGAAG AAAAATCCAA GCCCCTGAGT GAATCAGAAC TCATTGATGA GCTTTCAGAA 
GATTTTAACC AGCCTAAAAG TAAAGGAGAA CAGTCTAAGC CAACTGAAAA AACAGAAGAA 
TCTCAGGCTG CCCCCCTAAC TCCTGTGAGC GAGGCTGTGC CTCGGACCTC CATGTGTAGC 
ATACAGTCGG CACCGCCCAA GCCACCTACC TTGGAAGGCA TGGTGCCAGA TGATGCTGTA 
GAAGCCTTGG CTGGTAGCCT GGGGAAGAAG GAAGCAGATC CAGGAGATGG AAAGCCTGTG 
GAAGATAAAG TCAAGGAGAA AGCCAAGGAA GAGGATCGTG AAAAACTTGG TGAAAAAGAA 
GAAACAATTC CTCCTGATTA TAGATTAGAA GAGGTCAAGG ATAAAGATGG AAAACCACTC 
CTGCCAAAAG TGCCCAAGGA ATCGCTTTTA CCCATGAGTG AAGACTTCCT TCTTGATGCT 
TTGTCCAAGG ACTTTGCTGG TTCCTCAAAC ACTTCACCTC TTGAATTTGA AGAGGCTAAC 
CTTCCTGCTG TCATCTCTGA AGTGGTTTCC CAAACCCCAG CTCCAACCAC GCACTCTGCT 
GGTCTACCCC CTGATACTGT GCAAAGTGAC AACAAAGAAC TGGACGATGC TTTGGACCAA 
CTTTCTGACA GTCTGGGACA AAGGCAGCCT GATCCAGATG AGAACAAGCC GATAGAGGAT 
AAAGTCAAGG AAAGAGCTAA AGCTGAACAC AGAGACAAGC TGGGAGAAAG AGACGACACC 
ATCCCCCCTG AATATAGACA CCTCTTGGAT AACGATGAGG GTAAACCAGG AAAGCCACCT 
GCCAAGAAAA CCAAGGACTC AAAGAAGCCT GAAGATGACA AAGACCCCAT CGATGCCCTG 
TCAGGCGACT TCGACAGCTG TCCTGCACCC ACAGAAGCCT CAGAGAACAC AGCAGAGGAC 
AAAGCCAAGA GAACTGCTCC CAGCTCCAAA ACACCGAAGA ATAGGGGTAA AGCAAAGGAT 
TCTGCAAAGG CAAAGCAGGA AACTTCCAAG CCCAAAGCTG ATGAAAAAAA TACAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_014112200.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013967675.1 Canis lupus familiaris calpastatin (CAST), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OCe35587
Clone ID Related Accession (Same CDS sequence)	XM_014112200.1 , XM_014112200.2
Accession Version	XM_014112200.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	calpastatin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014112200.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTATTCCAG TCAGCAAACA GACGGGAGGA 
CAGCATTCTC CTAACAAGAA AAGACACAAA AAACAGGCTG CAAAAACAGA ACCTGAAAAG 
AAGTCACAAT CAACTAAGCC ATCTGTGGTC CATGAAAAAA AAACCCAAGA AGTAAAGCCA 
AAGGAACACA AAGAGCCAAA AAGCCTACCC AAGCATGCAT CAGATATGGG AAGCAAGCAT 
GTTCATAGTG AAAAAGCAGC TTCTAGATCA AGTGAGCAGC TGACATCAGA GAAATCAACA 
AAACCAAAGA CTAAAGCACA GGATTCCGTT TCTGCCGGTG GGGAGAGTGT TGTTGCAGGT 
GCAGCTGCAG CTGCAGCATC TGCCACAGCA GCTAACAAGT CAGACATGGA TGCTGCTTTG 
GATGACTTAG TAGACACTCT AGGAAAACCT GAAGAAACTA AAGAAGATAA TGTACCATAT 
ACTGGACCTG AAATTTCAGA TCCGATGACT TCCACCTACA TAGAGGAATT AGGTAAAAGA 
GAAGTCACAC TTCCTCCAAA GTACAGGGAA CTTTTGGCAA AAAAAGAAGG TATCACAGTG 
CCTCCTCCAG ACTCTTCGAA ACCCATGGGG CCCAATGATG CCATAGATGC TTTGTCATCT 
GACTTCACCT GCAGTTCACC TACCGATGCT GGAAAGAAAA CTAAGGAAGA GAAATCTACA 
GGAGAAGAGA TTTTAAAAGC CCAGCCAGCC AGGGTAACCA AAAGTGCTGC TTCACCCCAA 
GAGAAAAAAA GAAAGGTGGA GGAGCAGGAC GCAATGAGTG ATCAAGCACT GGAGGCTCTT 
TCAGCTTCTT TGGGCAGCCG GAAGCCAGAA CCCGAGCTGG ACCTCAGTTC CATTAAGGAA 
GTCGACGAGG CAAAAGCCAA AGAAGAGAAA CTAAAGAAGT GTGGTGAAGA TGAGGAAACT 
GTCCCATCAG AGTACAGATT AAAACCAGCT ACGGATAAAG ATGGAAAACC TCTATTGCCA 
GAGCCTGAAG AAAAATCCAA GCCCCTGAGT GAATCAGAAC TCATTGATGA GCTTTCAGAA 
GATTTTAACC AGCCTAAAAG TAAAGGAGAA CAGTCTAAGC CAACTGAAAA AACAGAAGAA 
TCTCAGGCTG CCCCCCTAAC TCCTGTGAGC GAGGCTGTGC CTCGGACCTC CATGTGTAGC 
ATACAGTCGG CACCGCCCAA GCCACCTACC TTGGAAGGCA TGGTGCCAGA TGATGCTGTA 
GAAGCCTTGG CTGGTAGCCT GGGGAAGAAG GAAGCAGATC CAGGAGATGG AAAGCCTGTG 
GAAGATAAAG TCAAGGAGAA AGCCAAGGAA GAGGATCGTG AAAAACTTGG TGAAAAAGAA 
GAAACAATTC CTCCTGATTA TAGATTAGAA GAGGTCAAGG ATAAAGATGG AAAACCACTC 
CTGCCAAAAG TGCCCAAGGA ATCGCTTTTA CCCATGAGTG AAGACTTCCT TCTTGATGCT 
TTGTCCAAGG ACTTTGCTGG TTCCTCAAAC ACTTCACCTC TTGAATTTGA AGAGGCTAAC 
CTTCCTGCTG TCATCTCTGA AGTGGTTTCC CAAACCCCAG CTCCAACCAC GCACTCTGCT 
GGTCTACCCC CTGATACTGT GCAAAGTGAC AACAAAGAAC TGGACGATGC TTTGGACCAA 
CTTTCTGACA GTCTGGGACA AAGGCAGCCT GATCCAGATG AGAACAAGCC GATAGAGGAT 
AAAGTCAAGG AAAGAGCTAA AGCTGAACAC AGAGACAAGC TGGGAGAAAG AGACGACACC 
ATCCCCCCTG AATATAGACA CCTCTTGGAT AACGATGAGG GTAAACCAGG AAAGCCACCT 
GCCAAGAAAA CCAAGGACTC AAAGAAGCCT GAAGATGACA AAGACCCCAT CGATGCCCTG 
TCAGGCGACT TCGACAGCTG TCCTGCACCC ACAGAAGCCT CAGAGAACAC AGCAGAGGAC 
AAAGCCAAGA GAACTGCTCC CAGCTCCAAA ACACCGAAGA ATAGGGGTAA AGCAAAGGAT 
TCTGCAAAGG CAAAGCAGGA AACTTCCAAG CCCAAAGCTG ATGAAAAAAA TACAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967675.1
CDS	79..2418
Translation	MSQPGQKPAASPRPRRAAAARDTQEHVSEKTSGSPSKSGEKKGADEKKARSLESSQPSRTHAGGTAPATKASASSASTSKSSSMNPTETKAIPVSKQTGGQHSPNKKRHKKQAAKTEPEKKSQSTKPSVVHEKKTQEVKPKEHKEPKSLPKHASDMGSKHVHSEKAASRSSEQLTSEKSTKPKTKAQDSVSAGGESVVAGAAAAAASATAANKSDMDAALDDLVDTLGKPEETKEDNVPYTGPEISDPMTSTYIEELGKREVTLPPKYRELLAKKEGITVPPPDSSKPMGPNDAIDALSSDFTCSSPTDAGKKTKEEKSTGEEILKAQPARVTKSAASPQEKKRKVEEQDAMSDQALEALSASLGSRKPEPELDLSSIKEVDEAKAKEEKLKKCGEDEETVPSEYRLKPATDKDGKPLLPEPEEKSKPLSESELIDELSEDFNQPKSKGEQSKPTEKTEESQAAPLTPVSEAVPRTSMCSIQSAPPKPPTLEGMVPDDAVEALAGSLGKKEADPGDGKPVEDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKVPKESLLPMSEDFLLDALSKDFAGSSNTSPLEFEEANLPAVISEVVSQTPAPTTHSAGLPPDTVQSDNKELDDALDQLSDSLGQRQPDPDENKPIEDKVKERAKAEHRDKLGERDDTIPPEYRHLLDNDEGKPGKPPAKKTKDSKKPEDDKDPIDALSGDFDSCPAPTEASENTAEDKAKRTAPSSKTPKNRGKAKDSAKAKQETSKPKADEKNTS

Target ORF information:

RefSeq Version	XM_014112200.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calpastatin (CAST), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014112200.2

ORF Insert Sequence:

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTATTCCAG TCAGCAAACA GACGGGAGGA 
CAGCATTCTC CTAACAAGAA AAGACACAAA AAACAGGCTG CAAAAACAGA ACCTGAAAAG 
AAGTCACAAT CAACTAAGCC ATCTGTGGTC CATGAAAAAA AAACCCAAGA AGTAAAGCCA 
AAGGAACACA AAGAGCCAAA AAGCCTACCC AAGCATGCAT CAGATATGGG AAGCAAGCAT 
GTTCATAGTG AAAAAGCAGC TTCTAGATCA AGTGAGCAGC TGACATCAGA GAAATCAACA 
AAACCAAAGA CTAAAGCACA GGATTCCGTT TCTGCCGGTG GGGAGAGTGT TGTTGCAGGT 
GCAGCTGCAG CTGCAGCATC TGCCACAGCA GCTAACAAGT CAGACATGGA TGCTGCTTTG 
GATGACTTAG TAGACACTCT AGGAAAACCT GAAGAAACTA AAGAAGATAA TGTACCATAT 
ACTGGACCTG AAATTTCAGA TCCGATGACT TCCACCTACA TAGAGGAATT AGGTAAAAGA 
GAAGTCACAC TTCCTCCAAA GTACAGGGAA CTTTTGGCAA AAAAAGAAGG TATCACAGTG 
CCTCCTCCAG ACTCTTCGAA ACCCATGGGG CCCAATGATG CCATAGATGC TTTGTCATCT 
GACTTCACCT GCAGTTCACC TACCGATGCT GGAAAGAAAA CTAAGGAAGA GAAATCTACA 
GGAGAAGAGA TTTTAAAAGC CCAGCCAGCC AGGGTAACCA AAAGTGCTGC TTCACCCCAA 
GAGAAAAAAA GAAAGGTGGA GGAGCAGGAC GCAATGAGTG ATCAAGCACT GGAGGCTCTT 
TCAGCTTCTT TGGGCAGCCG GAAGCCAGAA CCCGAGCTGG ACCTCAGTTC CATTAAGGAA 
GTCGACGAGG CAAAAGCCAA AGAAGAGAAA CTAAAGAAGT GTGGTGAAGA TGAGGAAACT 
GTCCCATCAG AGTACAGATT AAAACCAGCT ACGGATAAAG ATGGAAAACC TCTATTGCCA 
GAGCCTGAAG AAAAATCCAA GCCCCTGAGT GAATCAGAAC TCATTGATGA GCTTTCAGAA 
GATTTTAACC AGCCTAAAAG TAAAGGAGAA CAGTCTAAGC CAACTGAAAA AACAGAAGAA 
TCTCAGGCTG CCCCCCTAAC TCCTGTGAGC GAGGCTGTGC CTCGGACCTC CATGTGTAGC 
ATACAGTCGG CACCGCCCAA GCCACCTACC TTGGAAGGCA TGGTGCCAGA TGATGCTGTA 
GAAGCCTTGG CTGGTAGCCT GGGGAAGAAG GAAGCAGATC CAGGAGATGG AAAGCCTGTG 
GAAGATAAAG TCAAGGAGAA AGCCAAGGAA GAGGATCGTG AAAAACTTGG TGAAAAAGAA 
GAAACAATTC CTCCTGATTA TAGATTAGAA GAGGTCAAGG ATAAAGATGG AAAACCACTC 
CTGCCAAAAG TGCCCAAGGA ATCGCTTTTA CCCATGAGTG AAGACTTCCT TCTTGATGCT 
TTGTCCAAGG ACTTTGCTGG TTCCTCAAAC ACTTCACCTC TTGAATTTGA AGAGGCTAAC 
CTTCCTGCTG TCATCTCTGA AGTGGTTTCC CAAACCCCAG CTCCAACCAC GCACTCTGCT 
GGTCTACCCC CTGATACTGT GCAAAGTGAC AACAAAGAAC TGGACGATGC TTTGGACCAA 
CTTTCTGACA GTCTGGGACA AAGGCAGCCT GATCCAGATG AGAACAAGCC GATAGAGGAT 
AAAGTCAAGG AAAGAGCTAA AGCTGAACAC AGAGACAAGC TGGGAGAAAG AGACGACACC 
ATCCCCCCTG AATATAGACA CCTCTTGGAT AACGATGAGG GTAAACCAGG AAAGCCACCT 
GCCAAGAAAA CCAAGGACTC AAAGAAGCCT GAAGATGACA AAGACCCCAT CGATGCCCTG 
TCAGGCGACT TCGACAGCTG TCCTGCACCC ACAGAAGCCT CAGAGAACAC AGCAGAGGAC 
AAAGCCAAGA GAACTGCTCC CAGCTCCAAA ACACCGAAGA ATAGGGGTAA AGCAAAGGAT 
TCTGCAAAGG CAAAGCAGGA AACTTCCAAG CCCAAAGCTG ATGAAAAAAA TACAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_014112201.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013967676.1 Canis lupus familiaris calpastatin (CAST), transcript variant X10, mRNA.

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CloneID	OCe35588
Clone ID Related Accession (Same CDS sequence)	XM_014112201.1 , XM_014112201.2
Accession Version	XM_014112201.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	calpastatin isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726052.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGCAAAAA CAGAACCTGA AAAGAAGTCA 
CAATCAACTA AGCCATCTGT GGTCCATGAA AAAAAAACCC AAGAAGTAAA GCCAAAGGAA 
CACAAAGAGC CAAAAAGCCT ACCCAAGCAT GCATCAGATA TGGGAAGCAA GCATGTTCAT 
AGTGAAAAAG CAGCTTCTAG ATCAAGTGAG CAGCTGACAT CAGAGAAATC AACAAAACCA 
AAGACTAAAG CACAGGATTC CGTTTCTGCC GGTGGGGAGA GTGTTGTTGC AGGTGCAGCT 
GCAGCTGCAG CATCTGCCAC AGCAGCTAAC AAGAAAAAAG AAAAGAAATC ACTAACATCG 
GCTGTACCAG TTGAATCCAA ACCGGGTAAA CCACCTGGAA AGTCAGACAT GGATGCTGCT 
TTGGATGACT TAGTAGACAC TCTAGGAAAA CCTGAAGAAA CTAAAGAAGA TAATGTACCA 
TATACTGGAC CTGAAATTTC AGATCCGATG ACTTCCACCT ACATAGAGGA ATTAGGTAAA 
AGAGAAGTCA CACTTCCTCC AAAGTACAGG GAACTTTTGG CAAAAAAAGA AGGTATCACA 
GTGCCTCCTC CAGACTCTTC GAAACCCATG GGGCCCAATG ATGCCATAGA TGCTTTGTCA 
TCTGACTTCA CCTGCAGTTC ACCTACCGAT GCTGGAAAGA AAACTAAGGA AGAGAAATCT 
ACAGGAGAAG AGATTTTAAA AGCCCAGCCA GCCAGGGTAA CCAAAAGTGC TGCTTCACCC 
CAAGAGAAAA AAAGAAAGGT GGAGGAGGAC GCAATGAGTG ATCAAGCACT GGAGGCTCTT 
TCAGCTTCTT TGGGCAGCCG GAAGCCAGAA CCCGAGCTGG ACCTCAGTTC CATTAAGGAA 
GTCGACGAGG CAAAAGCCAA AGAAGAGAAA CTAAAGAAGT GTGGTGAAGA TGAGGAAACT 
GTCCCATCAG AGTACAGATT AAAACCAGCT ACGGATAAAG ATGGAAAACC TCTATTGCCA 
GAGCCTGAAG AAAAATCCAA GCCCCTGAGT GAATCAGAAC TCATTGATGA GCTTTCAGAA 
GATTTTAACC AGCCTAAAAG TAAAGGAGAA CAGTCTAAGC CAACTGAAAA AACAGAAGAA 
TCTCAGGCTG CCCCCCTAAC TCCTGTGAGC GAGGCTGTGC CTCGGACCTC CATGTGTAGC 
ATACAGTCGG CACCGCCCAA GCCACCTACC TTGGAAGGCA TGGTGCCAGA TGATGCTGTA 
GAAGCCTTGG CTGGTAGCCT GGGGAAGAAG GAAGCAGATC CAGGAGATGG AAAGCCTGTG 
GAAGATAAAG TCAAGGAGAA AGCCAAGGAA GAGGATCGTG AAAAACTTGG TGAAAAAGAA 
GAAACAATTC CTCCTGATTA TAGATTAGAA GAGGTCAAGG ATAAAGATGG AAAACCACTC 
CTGCCAAAAG TGCCCAAGGA ATCGCTTTTA CCCATGAGTG AAGACTTCCT TCTTGATGCT 
TTGTCCAAGG ACTTTGCTGG TTCCTCAAAC ACTTCACCTC TTGAATTTGA AGAGGCTAAC 
CTTCCTGCTG TCATCTCTGA AGTGGTTTCC CAAACCCCAG CTCCAACCAC GCACTCTGCT 
GGTCTACCCC CTGATACTGT GCAAAGTGAC AACAAAGAAC TGGACGATGC TTTGGACCAA 
CTTTCTGACA GTCTGGGACA AAGGCAGCCT GATCCAGATG AGAACAAGCC GATAGAGGAT 
AAAGTCAAGG AAAGAGCTAA AGCTGAACAC AGAGACAAGC TGGGAGAAAG AGACGACACC 
ATCCCCCCTG AATATAGACA CCTCTTGGAT AACGATGAGG GTAAACCAGG AAAGCCACCT 
GCCAAGAAAA CCAAGGACTC AAAGAAGCCT GAAGATGACA AAGACCCCAT CGATGCCCTG 
TCAGGCGACT TCGACAGCTG TCCTGCACCC ACAGAAGCCT CAGAGAACAC AGCAGAGGAC 
AAAGCCAAGA GAACTGCTCC CAGCTCCAAA ACACCGAAGA ATAGGGGTAA AGCAAAGGAT 
TCTGCAAAGG CAAAGCAGGA AACTTCCAAG CCCAAAGCTG ATGAAAAAAA TACAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967676.1
CDS	102..2441
Translation	MSQPGQKPAASPRPRRAAAARDTQEHVSEKTSGSPSKSGEKKGADEKKARSLESSQPSRTHAGGTAPATKASASSASTSKSSSMNPTETKAAKTEPEKKSQSTKPSVVHEKKTQEVKPKEHKEPKSLPKHASDMGSKHVHSEKAASRSSEQLTSEKSTKPKTKAQDSVSAGGESVVAGAAAAAASATAANKKKEKKSLTSAVPVESKPGKPPGKSDMDAALDDLVDTLGKPEETKEDNVPYTGPEISDPMTSTYIEELGKREVTLPPKYRELLAKKEGITVPPPDSSKPMGPNDAIDALSSDFTCSSPTDAGKKTKEEKSTGEEILKAQPARVTKSAASPQEKKRKVEEDAMSDQALEALSASLGSRKPEPELDLSSIKEVDEAKAKEEKLKKCGEDEETVPSEYRLKPATDKDGKPLLPEPEEKSKPLSESELIDELSEDFNQPKSKGEQSKPTEKTEESQAAPLTPVSEAVPRTSMCSIQSAPPKPPTLEGMVPDDAVEALAGSLGKKEADPGDGKPVEDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKVPKESLLPMSEDFLLDALSKDFAGSSNTSPLEFEEANLPAVISEVVSQTPAPTTHSAGLPPDTVQSDNKELDDALDQLSDSLGQRQPDPDENKPIEDKVKERAKAEHRDKLGERDDTIPPEYRHLLDNDEGKPGKPPAKKTKDSKKPEDDKDPIDALSGDFDSCPAPTEASENTAEDKAKRTAPSSKTPKNRGKAKDSAKAKQETSKPKADEKNTS

Target ORF information:

RefSeq Version	XM_014112201.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calpastatin (CAST), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014112201.1

ORF Insert Sequence:

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGCAAAAA CAGAACCTGA AAAGAAGTCA 
CAATCAACTA AGCCATCTGT GGTCCATGAA AAAAAAACCC AAGAAGTAAA GCCAAAGGAA 
CACAAAGAGC CAAAAAGCCT ACCCAAGCAT GCATCAGATA TGGGAAGCAA GCATGTTCAT 
AGTGAAAAAG CAGCTTCTAG ATCAAGTGAG CAGCTGACAT CAGAGAAATC AACAAAACCA 
AAGACTAAAG CACAGGATTC CGTTTCTGCC GGTGGGGAGA GTGTTGTTGC AGGTGCAGCT 
GCAGCTGCAG CATCTGCCAC AGCAGCTAAC AAGAAAAAAG AAAAGAAATC ACTAACATCG 
GCTGTACCAG TTGAATCCAA ACCGGGTAAA CCACCTGGAA AGTCAGACAT GGATGCTGCT 
TTGGATGACT TAGTAGACAC TCTAGGAAAA CCTGAAGAAA CTAAAGAAGA TAATGTACCA 
TATACTGGAC CTGAAATTTC AGATCCGATG ACTTCCACCT ACATAGAGGA ATTAGGTAAA 
AGAGAAGTCA CACTTCCTCC AAAGTACAGG GAACTTTTGG CAAAAAAAGA AGGTATCACA 
GTGCCTCCTC CAGACTCTTC GAAACCCATG GGGCCCAATG ATGCCATAGA TGCTTTGTCA 
TCTGACTTCA CCTGCAGTTC ACCTACCGAT GCTGGAAAGA AAACTAAGGA AGAGAAATCT 
ACAGGAGAAG AGATTTTAAA AGCCCAGCCA GCCAGGGTAA CCAAAAGTGC TGCTTCACCC 
CAAGAGAAAA AAAGAAAGGT GGAGGAGGAC GCAATGAGTG ATCAAGCACT GGAGGCTCTT 
TCAGCTTCTT TGGGCAGCCG GAAGCCAGAA CCCGAGCTGG ACCTCAGTTC CATTAAGGAA 
GTCGACGAGG CAAAAGCCAA AGAAGAGAAA CTAAAGAAGT GTGGTGAAGA TGAGGAAACT 
GTCCCATCAG AGTACAGATT AAAACCAGCT ACGGATAAAG ATGGAAAACC TCTATTGCCA 
GAGCCTGAAG AAAAATCCAA GCCCCTGAGT GAATCAGAAC TCATTGATGA GCTTTCAGAA 
GATTTTAACC AGCCTAAAAG TAAAGGAGAA CAGTCTAAGC CAACTGAAAA AACAGAAGAA 
TCTCAGGCTG CCCCCCTAAC TCCTGTGAGC GAGGCTGTGC CTCGGACCTC CATGTGTAGC 
ATACAGTCGG CACCGCCCAA GCCACCTACC TTGGAAGGCA TGGTGCCAGA TGATGCTGTA 
GAAGCCTTGG CTGGTAGCCT GGGGAAGAAG GAAGCAGATC CAGGAGATGG AAAGCCTGTG 
GAAGATAAAG TCAAGGAGAA AGCCAAGGAA GAGGATCGTG AAAAACTTGG TGAAAAAGAA 
GAAACAATTC CTCCTGATTA TAGATTAGAA GAGGTCAAGG ATAAAGATGG AAAACCACTC 
CTGCCAAAAG TGCCCAAGGA ATCGCTTTTA CCCATGAGTG AAGACTTCCT TCTTGATGCT 
TTGTCCAAGG ACTTTGCTGG TTCCTCAAAC ACTTCACCTC TTGAATTTGA AGAGGCTAAC 
CTTCCTGCTG TCATCTCTGA AGTGGTTTCC CAAACCCCAG CTCCAACCAC GCACTCTGCT 
GGTCTACCCC CTGATACTGT GCAAAGTGAC AACAAAGAAC TGGACGATGC TTTGGACCAA 
CTTTCTGACA GTCTGGGACA AAGGCAGCCT GATCCAGATG AGAACAAGCC GATAGAGGAT 
AAAGTCAAGG AAAGAGCTAA AGCTGAACAC AGAGACAAGC TGGGAGAAAG AGACGACACC 
ATCCCCCCTG AATATAGACA CCTCTTGGAT AACGATGAGG GTAAACCAGG AAAGCCACCT 
GCCAAGAAAA CCAAGGACTC AAAGAAGCCT GAAGATGACA AAGACCCCAT CGATGCCCTG 
TCAGGCGACT TCGACAGCTG TCCTGCACCC ACAGAAGCCT CAGAGAACAC AGCAGAGGAC 
AAAGCCAAGA GAACTGCTCC CAGCTCCAAA ACACCGAAGA ATAGGGGTAA AGCAAAGGAT 
TCTGCAAAGG CAAAGCAGGA AACTTCCAAG CCCAAAGCTG ATGAAAAAAA TACAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_014112201.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013967676.1 Canis lupus familiaris calpastatin (CAST), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OCe35588
Clone ID Related Accession (Same CDS sequence)	XM_014112201.1 , XM_014112201.2
Accession Version	XM_014112201.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	calpastatin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014112201.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGCAAAAA CAGAACCTGA AAAGAAGTCA 
CAATCAACTA AGCCATCTGT GGTCCATGAA AAAAAAACCC AAGAAGTAAA GCCAAAGGAA 
CACAAAGAGC CAAAAAGCCT ACCCAAGCAT GCATCAGATA TGGGAAGCAA GCATGTTCAT 
AGTGAAAAAG CAGCTTCTAG ATCAAGTGAG CAGCTGACAT CAGAGAAATC AACAAAACCA 
AAGACTAAAG CACAGGATTC CGTTTCTGCC GGTGGGGAGA GTGTTGTTGC AGGTGCAGCT 
GCAGCTGCAG CATCTGCCAC AGCAGCTAAC AAGAAAAAAG AAAAGAAATC ACTAACATCG 
GCTGTACCAG TTGAATCCAA ACCGGGTAAA CCACCTGGAA AGTCAGACAT GGATGCTGCT 
TTGGATGACT TAGTAGACAC TCTAGGAAAA CCTGAAGAAA CTAAAGAAGA TAATGTACCA 
TATACTGGAC CTGAAATTTC AGATCCGATG ACTTCCACCT ACATAGAGGA ATTAGGTAAA 
AGAGAAGTCA CACTTCCTCC AAAGTACAGG GAACTTTTGG CAAAAAAAGA AGGTATCACA 
GTGCCTCCTC CAGACTCTTC GAAACCCATG GGGCCCAATG ATGCCATAGA TGCTTTGTCA 
TCTGACTTCA CCTGCAGTTC ACCTACCGAT GCTGGAAAGA AAACTAAGGA AGAGAAATCT 
ACAGGAGAAG AGATTTTAAA AGCCCAGCCA GCCAGGGTAA CCAAAAGTGC TGCTTCACCC 
CAAGAGAAAA AAAGAAAGGT GGAGGAGGAC GCAATGAGTG ATCAAGCACT GGAGGCTCTT 
TCAGCTTCTT TGGGCAGCCG GAAGCCAGAA CCCGAGCTGG ACCTCAGTTC CATTAAGGAA 
GTCGACGAGG CAAAAGCCAA AGAAGAGAAA CTAAAGAAGT GTGGTGAAGA TGAGGAAACT 
GTCCCATCAG AGTACAGATT AAAACCAGCT ACGGATAAAG ATGGAAAACC TCTATTGCCA 
GAGCCTGAAG AAAAATCCAA GCCCCTGAGT GAATCAGAAC TCATTGATGA GCTTTCAGAA 
GATTTTAACC AGCCTAAAAG TAAAGGAGAA CAGTCTAAGC CAACTGAAAA AACAGAAGAA 
TCTCAGGCTG CCCCCCTAAC TCCTGTGAGC GAGGCTGTGC CTCGGACCTC CATGTGTAGC 
ATACAGTCGG CACCGCCCAA GCCACCTACC TTGGAAGGCA TGGTGCCAGA TGATGCTGTA 
GAAGCCTTGG CTGGTAGCCT GGGGAAGAAG GAAGCAGATC CAGGAGATGG AAAGCCTGTG 
GAAGATAAAG TCAAGGAGAA AGCCAAGGAA GAGGATCGTG AAAAACTTGG TGAAAAAGAA 
GAAACAATTC CTCCTGATTA TAGATTAGAA GAGGTCAAGG ATAAAGATGG AAAACCACTC 
CTGCCAAAAG TGCCCAAGGA ATCGCTTTTA CCCATGAGTG AAGACTTCCT TCTTGATGCT 
TTGTCCAAGG ACTTTGCTGG TTCCTCAAAC ACTTCACCTC TTGAATTTGA AGAGGCTAAC 
CTTCCTGCTG TCATCTCTGA AGTGGTTTCC CAAACCCCAG CTCCAACCAC GCACTCTGCT 
GGTCTACCCC CTGATACTGT GCAAAGTGAC AACAAAGAAC TGGACGATGC TTTGGACCAA 
CTTTCTGACA GTCTGGGACA AAGGCAGCCT GATCCAGATG AGAACAAGCC GATAGAGGAT 
AAAGTCAAGG AAAGAGCTAA AGCTGAACAC AGAGACAAGC TGGGAGAAAG AGACGACACC 
ATCCCCCCTG AATATAGACA CCTCTTGGAT AACGATGAGG GTAAACCAGG AAAGCCACCT 
GCCAAGAAAA CCAAGGACTC AAAGAAGCCT GAAGATGACA AAGACCCCAT CGATGCCCTG 
TCAGGCGACT TCGACAGCTG TCCTGCACCC ACAGAAGCCT CAGAGAACAC AGCAGAGGAC 
AAAGCCAAGA GAACTGCTCC CAGCTCCAAA ACACCGAAGA ATAGGGGTAA AGCAAAGGAT 
TCTGCAAAGG CAAAGCAGGA AACTTCCAAG CCCAAAGCTG ATGAAAAAAA TACAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967676.1
CDS	79..2418
Translation	MSQPGQKPAASPRPRRAAAARDTQEHVSEKTSGSPSKSGEKKGADEKKARSLESSQPSRTHAGGTAPATKASASSASTSKSSSMNPTETKAAKTEPEKKSQSTKPSVVHEKKTQEVKPKEHKEPKSLPKHASDMGSKHVHSEKAASRSSEQLTSEKSTKPKTKAQDSVSAGGESVVAGAAAAAASATAANKKKEKKSLTSAVPVESKPGKPPGKSDMDAALDDLVDTLGKPEETKEDNVPYTGPEISDPMTSTYIEELGKREVTLPPKYRELLAKKEGITVPPPDSSKPMGPNDAIDALSSDFTCSSPTDAGKKTKEEKSTGEEILKAQPARVTKSAASPQEKKRKVEEDAMSDQALEALSASLGSRKPEPELDLSSIKEVDEAKAKEEKLKKCGEDEETVPSEYRLKPATDKDGKPLLPEPEEKSKPLSESELIDELSEDFNQPKSKGEQSKPTEKTEESQAAPLTPVSEAVPRTSMCSIQSAPPKPPTLEGMVPDDAVEALAGSLGKKEADPGDGKPVEDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKVPKESLLPMSEDFLLDALSKDFAGSSNTSPLEFEEANLPAVISEVVSQTPAPTTHSAGLPPDTVQSDNKELDDALDQLSDSLGQRQPDPDENKPIEDKVKERAKAEHRDKLGERDDTIPPEYRHLLDNDEGKPGKPPAKKTKDSKKPEDDKDPIDALSGDFDSCPAPTEASENTAEDKAKRTAPSSKTPKNRGKAKDSAKAKQETSKPKADEKNTS

Target ORF information:

RefSeq Version	XM_014112201.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calpastatin (CAST), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014112201.2

ORF Insert Sequence:

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGCAAAAA CAGAACCTGA AAAGAAGTCA 
CAATCAACTA AGCCATCTGT GGTCCATGAA AAAAAAACCC AAGAAGTAAA GCCAAAGGAA 
CACAAAGAGC CAAAAAGCCT ACCCAAGCAT GCATCAGATA TGGGAAGCAA GCATGTTCAT 
AGTGAAAAAG CAGCTTCTAG ATCAAGTGAG CAGCTGACAT CAGAGAAATC AACAAAACCA 
AAGACTAAAG CACAGGATTC CGTTTCTGCC GGTGGGGAGA GTGTTGTTGC AGGTGCAGCT 
GCAGCTGCAG CATCTGCCAC AGCAGCTAAC AAGAAAAAAG AAAAGAAATC ACTAACATCG 
GCTGTACCAG TTGAATCCAA ACCGGGTAAA CCACCTGGAA AGTCAGACAT GGATGCTGCT 
TTGGATGACT TAGTAGACAC TCTAGGAAAA CCTGAAGAAA CTAAAGAAGA TAATGTACCA 
TATACTGGAC CTGAAATTTC AGATCCGATG ACTTCCACCT ACATAGAGGA ATTAGGTAAA 
AGAGAAGTCA CACTTCCTCC AAAGTACAGG GAACTTTTGG CAAAAAAAGA AGGTATCACA 
GTGCCTCCTC CAGACTCTTC GAAACCCATG GGGCCCAATG ATGCCATAGA TGCTTTGTCA 
TCTGACTTCA CCTGCAGTTC ACCTACCGAT GCTGGAAAGA AAACTAAGGA AGAGAAATCT 
ACAGGAGAAG AGATTTTAAA AGCCCAGCCA GCCAGGGTAA CCAAAAGTGC TGCTTCACCC 
CAAGAGAAAA AAAGAAAGGT GGAGGAGGAC GCAATGAGTG ATCAAGCACT GGAGGCTCTT 
TCAGCTTCTT TGGGCAGCCG GAAGCCAGAA CCCGAGCTGG ACCTCAGTTC CATTAAGGAA 
GTCGACGAGG CAAAAGCCAA AGAAGAGAAA CTAAAGAAGT GTGGTGAAGA TGAGGAAACT 
GTCCCATCAG AGTACAGATT AAAACCAGCT ACGGATAAAG ATGGAAAACC TCTATTGCCA 
GAGCCTGAAG AAAAATCCAA GCCCCTGAGT GAATCAGAAC TCATTGATGA GCTTTCAGAA 
GATTTTAACC AGCCTAAAAG TAAAGGAGAA CAGTCTAAGC CAACTGAAAA AACAGAAGAA 
TCTCAGGCTG CCCCCCTAAC TCCTGTGAGC GAGGCTGTGC CTCGGACCTC CATGTGTAGC 
ATACAGTCGG CACCGCCCAA GCCACCTACC TTGGAAGGCA TGGTGCCAGA TGATGCTGTA 
GAAGCCTTGG CTGGTAGCCT GGGGAAGAAG GAAGCAGATC CAGGAGATGG AAAGCCTGTG 
GAAGATAAAG TCAAGGAGAA AGCCAAGGAA GAGGATCGTG AAAAACTTGG TGAAAAAGAA 
GAAACAATTC CTCCTGATTA TAGATTAGAA GAGGTCAAGG ATAAAGATGG AAAACCACTC 
CTGCCAAAAG TGCCCAAGGA ATCGCTTTTA CCCATGAGTG AAGACTTCCT TCTTGATGCT 
TTGTCCAAGG ACTTTGCTGG TTCCTCAAAC ACTTCACCTC TTGAATTTGA AGAGGCTAAC 
CTTCCTGCTG TCATCTCTGA AGTGGTTTCC CAAACCCCAG CTCCAACCAC GCACTCTGCT 
GGTCTACCCC CTGATACTGT GCAAAGTGAC AACAAAGAAC TGGACGATGC TTTGGACCAA 
CTTTCTGACA GTCTGGGACA AAGGCAGCCT GATCCAGATG AGAACAAGCC GATAGAGGAT 
AAAGTCAAGG AAAGAGCTAA AGCTGAACAC AGAGACAAGC TGGGAGAAAG AGACGACACC 
ATCCCCCCTG AATATAGACA CCTCTTGGAT AACGATGAGG GTAAACCAGG AAAGCCACCT 
GCCAAGAAAA CCAAGGACTC AAAGAAGCCT GAAGATGACA AAGACCCCAT CGATGCCCTG 
TCAGGCGACT TCGACAGCTG TCCTGCACCC ACAGAAGCCT CAGAGAACAC AGCAGAGGAC 
AAAGCCAAGA GAACTGCTCC CAGCTCCAAA ACACCGAAGA ATAGGGGTAA AGCAAAGGAT 
TCTGCAAAGG CAAAGCAGGA AACTTCCAAG CCCAAAGCTG ATGAAAAAAA TACAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_014112202.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013967677.1 Canis lupus familiaris calpastatin (CAST), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OCe35589
Clone ID Related Accession (Same CDS sequence)	XM_014112202.2 , XM_014112202.1
Accession Version	XM_014112202.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	calpastatin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014112202.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGCAAAAA CAGAACCTGA AAAGAAGTCA 
CAATCAACTA AGCCAAAAAG CCTACCCAAG CATGCATCAG ATATGGGAAG CAAGCATGTT 
CATAGTGAAA AAGCAGCTTC TAGATCAAGT GAGCAGCTGA CATCAGAGAA ATCAACAAAA 
CCAAAGACTA AAGCACAGGA TTCCGTTTCT GCCGGTGGGG AGAGTGTTGT TGCAGGTGCA 
GCTGCAGCTG CAGCATCTGC CACAGCAGCT AACAAGCAGA AAAAAGAAAA GAAATCACTA 
ACATCGGCTG TACCAGTTGA ATCCAAACCG GGTAAACCAC CTGGAAAGTC AGACATGGAT 
GCTGCTTTGG ATGACTTAGT AGACACTCTA GGAAAACCTG AAGAAACTAA AGAAGATAAT 
GTACCATATA CTGGACCTGA AATTTCAGAT CCGATGACTT CCACCTACAT AGAGGAATTA 
GGTAAAAGAG AAGTCACACT TCCTCCAAAG TACAGGGAAC TTTTGGCAAA AAAAGAAGGT 
ATCACAGTGC CTCCTCCAGA CTCTTCGAAA CCCATGGGGC CCAATGATGC CATAGATGCT 
TTGTCATCTG ACTTCACCTG CAGTTCACCT ACCGATGCTG GAAAGAAAAC TAAGGAAGAG 
AAATCTACAG GAGAAGAGAT TTTAAAAGCC CAGCCAGCCA GGGTAACCAA AAGTGCTGCT 
TCACCCCAAG AGAAAAAAAG AAAGGTGGAG GAGCAGGACG CAATGAGTGA TCAAGCACTG 
GAGGCTCTTT CAGCTTCTTT GGGCAGCCGG AAGCCAGAAC CCGAGCTGGA CCTCAGTTCC 
ATTAAGGAAG TCGACGAGGC AAAAGCCAAA GAAGAGAAAC TAAAGAAGTG TGGTGAAGAT 
GAGGAAACTG TCCCATCAGA GTACAGATTA AAACCAGCTA CGGATAAAGA TGGAAAACCT 
CTATTGCCAG AGCCTGAAGA AAAATCCAAG CCCCTGAGTG AATCAGAACT CATTGATGAG 
CTTTCAGAAG ATTTTAACCA GCCTAAAAGT AAAGGAGAAC AGTCTAAGCC AACTGAAAAA 
ACAGAAGAAT CTCAGGCTGC CCCCCTAACT CCTGTGAGCG AGGCTGTGCC TCGGACCTCC 
ATGTGTAGCA TACAGTCGGC ACCGCCCAAG CCACCTACCT TGGAAGGCAT GGTGCCAGAT 
GATGCTGTAG AAGCCTTGGC TGGTAGCCTG GGGAAGAAGG AAGCAGATCC AGGAGATGGA 
AAGCCTGTGG AAGATAAAGT CAAGGAGAAA GCCAAGGAAG AGGATCGTGA AAAACTTGGT 
GAAAAAGAAG AAACAATTCC TCCTGATTAT AGATTAGAAG AGGTCAAGGA TAAAGATGGA 
AAACCACTCC TGCCAAAAGT GCCCAAGGAA TCGCTTTTAC CCATGAGTGA AGACTTCCTT 
CTTGATGCTT TGTCCAAGGA CTTTGCTGGT TCCTCAAACA CTTCACCTCT TGAATTTGAA 
GAGGCTAACC TTCCTGCTGT CATCTCTGAA GTGGTTTCCC AAACCCCAGC TCCAACCACG 
CACTCTGCTG GTCTACCCCC TGATACTGTG CAAAGTGACA ACAAAGAACT GGACGATGCT 
TTGGACCAAC TTTCTGACAG TCTGGGACAA AGGCAGCCTG ATCCAGATGA GAACAAGCCG 
ATAGAGGATA AAGTCAAGGA AAGAGCTAAA GCTGAACACA GAGACAAGCT GGGAGAAAGA 
GACGACACCA TCCCCCCTGA ATATAGACAC CTCTTGGATA ACGATGAGGG TAAACCAGGA 
AAGCCACCTG CCAAGAAAAC CAAGGACTCA AAGAAGCCTG AAGATGACAA AGACCCCATC 
GATGCCCTGT CAGGCGACTT CGACAGCTGT CCTGCACCCA CAGAAGCCTC AGAGAACACA 
GCAGAGGACA AAGCCAAGAG AACTGCTCCC AGCTCCAAAA CACCGAAGAA TAGGGGTAAA 
GCAAAGGATT CTGCAAAGGC AAAGCAGGAA ACTTCCAAGC CCAAAGCTGA TGAAAAAAAT 
ACAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967677.1
CDS	79..2367
Translation	MSQPGQKPAASPRPRRAAAARDTQEHVSEKTSGSPSKSGEKKGADEKKARSLESSQPSRTHAGGTAPATKASASSASTSKSSSMNPTETKAAKTEPEKKSQSTKPKSLPKHASDMGSKHVHSEKAASRSSEQLTSEKSTKPKTKAQDSVSAGGESVVAGAAAAAASATAANKQKKEKKSLTSAVPVESKPGKPPGKSDMDAALDDLVDTLGKPEETKEDNVPYTGPEISDPMTSTYIEELGKREVTLPPKYRELLAKKEGITVPPPDSSKPMGPNDAIDALSSDFTCSSPTDAGKKTKEEKSTGEEILKAQPARVTKSAASPQEKKRKVEEQDAMSDQALEALSASLGSRKPEPELDLSSIKEVDEAKAKEEKLKKCGEDEETVPSEYRLKPATDKDGKPLLPEPEEKSKPLSESELIDELSEDFNQPKSKGEQSKPTEKTEESQAAPLTPVSEAVPRTSMCSIQSAPPKPPTLEGMVPDDAVEALAGSLGKKEADPGDGKPVEDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKVPKESLLPMSEDFLLDALSKDFAGSSNTSPLEFEEANLPAVISEVVSQTPAPTTHSAGLPPDTVQSDNKELDDALDQLSDSLGQRQPDPDENKPIEDKVKERAKAEHRDKLGERDDTIPPEYRHLLDNDEGKPGKPPAKKTKDSKKPEDDKDPIDALSGDFDSCPAPTEASENTAEDKAKRTAPSSKTPKNRGKAKDSAKAKQETSKPKADEKNTS

Target ORF information:

RefSeq Version	XM_014112202.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calpastatin (CAST), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014112202.2

ORF Insert Sequence:

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGCAAAAA CAGAACCTGA AAAGAAGTCA 
CAATCAACTA AGCCAAAAAG CCTACCCAAG CATGCATCAG ATATGGGAAG CAAGCATGTT 
CATAGTGAAA AAGCAGCTTC TAGATCAAGT GAGCAGCTGA CATCAGAGAA ATCAACAAAA 
CCAAAGACTA AAGCACAGGA TTCCGTTTCT GCCGGTGGGG AGAGTGTTGT TGCAGGTGCA 
GCTGCAGCTG CAGCATCTGC CACAGCAGCT AACAAGCAGA AAAAAGAAAA GAAATCACTA 
ACATCGGCTG TACCAGTTGA ATCCAAACCG GGTAAACCAC CTGGAAAGTC AGACATGGAT 
GCTGCTTTGG ATGACTTAGT AGACACTCTA GGAAAACCTG AAGAAACTAA AGAAGATAAT 
GTACCATATA CTGGACCTGA AATTTCAGAT CCGATGACTT CCACCTACAT AGAGGAATTA 
GGTAAAAGAG AAGTCACACT TCCTCCAAAG TACAGGGAAC TTTTGGCAAA AAAAGAAGGT 
ATCACAGTGC CTCCTCCAGA CTCTTCGAAA CCCATGGGGC CCAATGATGC CATAGATGCT 
TTGTCATCTG ACTTCACCTG CAGTTCACCT ACCGATGCTG GAAAGAAAAC TAAGGAAGAG 
AAATCTACAG GAGAAGAGAT TTTAAAAGCC CAGCCAGCCA GGGTAACCAA AAGTGCTGCT 
TCACCCCAAG AGAAAAAAAG AAAGGTGGAG GAGCAGGACG CAATGAGTGA TCAAGCACTG 
GAGGCTCTTT CAGCTTCTTT GGGCAGCCGG AAGCCAGAAC CCGAGCTGGA CCTCAGTTCC 
ATTAAGGAAG TCGACGAGGC AAAAGCCAAA GAAGAGAAAC TAAAGAAGTG TGGTGAAGAT 
GAGGAAACTG TCCCATCAGA GTACAGATTA AAACCAGCTA CGGATAAAGA TGGAAAACCT 
CTATTGCCAG AGCCTGAAGA AAAATCCAAG CCCCTGAGTG AATCAGAACT CATTGATGAG 
CTTTCAGAAG ATTTTAACCA GCCTAAAAGT AAAGGAGAAC AGTCTAAGCC AACTGAAAAA 
ACAGAAGAAT CTCAGGCTGC CCCCCTAACT CCTGTGAGCG AGGCTGTGCC TCGGACCTCC 
ATGTGTAGCA TACAGTCGGC ACCGCCCAAG CCACCTACCT TGGAAGGCAT GGTGCCAGAT 
GATGCTGTAG AAGCCTTGGC TGGTAGCCTG GGGAAGAAGG AAGCAGATCC AGGAGATGGA 
AAGCCTGTGG AAGATAAAGT CAAGGAGAAA GCCAAGGAAG AGGATCGTGA AAAACTTGGT 
GAAAAAGAAG AAACAATTCC TCCTGATTAT AGATTAGAAG AGGTCAAGGA TAAAGATGGA 
AAACCACTCC TGCCAAAAGT GCCCAAGGAA TCGCTTTTAC CCATGAGTGA AGACTTCCTT 
CTTGATGCTT TGTCCAAGGA CTTTGCTGGT TCCTCAAACA CTTCACCTCT TGAATTTGAA 
GAGGCTAACC TTCCTGCTGT CATCTCTGAA GTGGTTTCCC AAACCCCAGC TCCAACCACG 
CACTCTGCTG GTCTACCCCC TGATACTGTG CAAAGTGACA ACAAAGAACT GGACGATGCT 
TTGGACCAAC TTTCTGACAG TCTGGGACAA AGGCAGCCTG ATCCAGATGA GAACAAGCCG 
ATAGAGGATA AAGTCAAGGA AAGAGCTAAA GCTGAACACA GAGACAAGCT GGGAGAAAGA 
GACGACACCA TCCCCCCTGA ATATAGACAC CTCTTGGATA ACGATGAGGG TAAACCAGGA 
AAGCCACCTG CCAAGAAAAC CAAGGACTCA AAGAAGCCTG AAGATGACAA AGACCCCATC 
GATGCCCTGT CAGGCGACTT CGACAGCTGT CCTGCACCCA CAGAAGCCTC AGAGAACACA 
GCAGAGGACA AAGCCAAGAG AACTGCTCCC AGCTCCAAAA CACCGAAGAA TAGGGGTAAA 
GCAAAGGATT CTGCAAAGGC AAAGCAGGAA ACTTCCAAGC CCAAAGCTGA TGAAAAAAAT 
ACAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_014112202.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013967677.1 Canis lupus familiaris calpastatin (CAST), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OCe35589
Clone ID Related Accession (Same CDS sequence)	XM_014112202.2 , XM_014112202.1
Accession Version	XM_014112202.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	calpastatin isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726052.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGCAAAAA CAGAACCTGA AAAGAAGTCA 
CAATCAACTA AGCCAAAAAG CCTACCCAAG CATGCATCAG ATATGGGAAG CAAGCATGTT 
CATAGTGAAA AAGCAGCTTC TAGATCAAGT GAGCAGCTGA CATCAGAGAA ATCAACAAAA 
CCAAAGACTA AAGCACAGGA TTCCGTTTCT GCCGGTGGGG AGAGTGTTGT TGCAGGTGCA 
GCTGCAGCTG CAGCATCTGC CACAGCAGCT AACAAGCAGA AAAAAGAAAA GAAATCACTA 
ACATCGGCTG TACCAGTTGA ATCCAAACCG GGTAAACCAC CTGGAAAGTC AGACATGGAT 
GCTGCTTTGG ATGACTTAGT AGACACTCTA GGAAAACCTG AAGAAACTAA AGAAGATAAT 
GTACCATATA CTGGACCTGA AATTTCAGAT CCGATGACTT CCACCTACAT AGAGGAATTA 
GGTAAAAGAG AAGTCACACT TCCTCCAAAG TACAGGGAAC TTTTGGCAAA AAAAGAAGGT 
ATCACAGTGC CTCCTCCAGA CTCTTCGAAA CCCATGGGGC CCAATGATGC CATAGATGCT 
TTGTCATCTG ACTTCACCTG CAGTTCACCT ACCGATGCTG GAAAGAAAAC TAAGGAAGAG 
AAATCTACAG GAGAAGAGAT TTTAAAAGCC CAGCCAGCCA GGGTAACCAA AAGTGCTGCT 
TCACCCCAAG AGAAAAAAAG AAAGGTGGAG GAGCAGGACG CAATGAGTGA TCAAGCACTG 
GAGGCTCTTT CAGCTTCTTT GGGCAGCCGG AAGCCAGAAC CCGAGCTGGA CCTCAGTTCC 
ATTAAGGAAG TCGACGAGGC AAAAGCCAAA GAAGAGAAAC TAAAGAAGTG TGGTGAAGAT 
GAGGAAACTG TCCCATCAGA GTACAGATTA AAACCAGCTA CGGATAAAGA TGGAAAACCT 
CTATTGCCAG AGCCTGAAGA AAAATCCAAG CCCCTGAGTG AATCAGAACT CATTGATGAG 
CTTTCAGAAG ATTTTAACCA GCCTAAAAGT AAAGGAGAAC AGTCTAAGCC AACTGAAAAA 
ACAGAAGAAT CTCAGGCTGC CCCCCTAACT CCTGTGAGCG AGGCTGTGCC TCGGACCTCC 
ATGTGTAGCA TACAGTCGGC ACCGCCCAAG CCACCTACCT TGGAAGGCAT GGTGCCAGAT 
GATGCTGTAG AAGCCTTGGC TGGTAGCCTG GGGAAGAAGG AAGCAGATCC AGGAGATGGA 
AAGCCTGTGG AAGATAAAGT CAAGGAGAAA GCCAAGGAAG AGGATCGTGA AAAACTTGGT 
GAAAAAGAAG AAACAATTCC TCCTGATTAT AGATTAGAAG AGGTCAAGGA TAAAGATGGA 
AAACCACTCC TGCCAAAAGT GCCCAAGGAA TCGCTTTTAC CCATGAGTGA AGACTTCCTT 
CTTGATGCTT TGTCCAAGGA CTTTGCTGGT TCCTCAAACA CTTCACCTCT TGAATTTGAA 
GAGGCTAACC TTCCTGCTGT CATCTCTGAA GTGGTTTCCC AAACCCCAGC TCCAACCACG 
CACTCTGCTG GTCTACCCCC TGATACTGTG CAAAGTGACA ACAAAGAACT GGACGATGCT 
TTGGACCAAC TTTCTGACAG TCTGGGACAA AGGCAGCCTG ATCCAGATGA GAACAAGCCG 
ATAGAGGATA AAGTCAAGGA AAGAGCTAAA GCTGAACACA GAGACAAGCT GGGAGAAAGA 
GACGACACCA TCCCCCCTGA ATATAGACAC CTCTTGGATA ACGATGAGGG TAAACCAGGA 
AAGCCACCTG CCAAGAAAAC CAAGGACTCA AAGAAGCCTG AAGATGACAA AGACCCCATC 
GATGCCCTGT CAGGCGACTT CGACAGCTGT CCTGCACCCA CAGAAGCCTC AGAGAACACA 
GCAGAGGACA AAGCCAAGAG AACTGCTCCC AGCTCCAAAA CACCGAAGAA TAGGGGTAAA 
GCAAAGGATT CTGCAAAGGC AAAGCAGGAA ACTTCCAAGC CCAAAGCTGA TGAAAAAAAT 
ACAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967677.1
CDS	102..2390
Translation	MSQPGQKPAASPRPRRAAAARDTQEHVSEKTSGSPSKSGEKKGADEKKARSLESSQPSRTHAGGTAPATKASASSASTSKSSSMNPTETKAAKTEPEKKSQSTKPKSLPKHASDMGSKHVHSEKAASRSSEQLTSEKSTKPKTKAQDSVSAGGESVVAGAAAAAASATAANKQKKEKKSLTSAVPVESKPGKPPGKSDMDAALDDLVDTLGKPEETKEDNVPYTGPEISDPMTSTYIEELGKREVTLPPKYRELLAKKEGITVPPPDSSKPMGPNDAIDALSSDFTCSSPTDAGKKTKEEKSTGEEILKAQPARVTKSAASPQEKKRKVEEQDAMSDQALEALSASLGSRKPEPELDLSSIKEVDEAKAKEEKLKKCGEDEETVPSEYRLKPATDKDGKPLLPEPEEKSKPLSESELIDELSEDFNQPKSKGEQSKPTEKTEESQAAPLTPVSEAVPRTSMCSIQSAPPKPPTLEGMVPDDAVEALAGSLGKKEADPGDGKPVEDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKVPKESLLPMSEDFLLDALSKDFAGSSNTSPLEFEEANLPAVISEVVSQTPAPTTHSAGLPPDTVQSDNKELDDALDQLSDSLGQRQPDPDENKPIEDKVKERAKAEHRDKLGERDDTIPPEYRHLLDNDEGKPGKPPAKKTKDSKKPEDDKDPIDALSGDFDSCPAPTEASENTAEDKAKRTAPSSKTPKNRGKAKDSAKAKQETSKPKADEKNTS

Target ORF information:

RefSeq Version	XM_014112202.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calpastatin (CAST), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014112202.1

ORF Insert Sequence:

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGCAAAAA CAGAACCTGA AAAGAAGTCA 
CAATCAACTA AGCCAAAAAG CCTACCCAAG CATGCATCAG ATATGGGAAG CAAGCATGTT 
CATAGTGAAA AAGCAGCTTC TAGATCAAGT GAGCAGCTGA CATCAGAGAA ATCAACAAAA 
CCAAAGACTA AAGCACAGGA TTCCGTTTCT GCCGGTGGGG AGAGTGTTGT TGCAGGTGCA 
GCTGCAGCTG CAGCATCTGC CACAGCAGCT AACAAGCAGA AAAAAGAAAA GAAATCACTA 
ACATCGGCTG TACCAGTTGA ATCCAAACCG GGTAAACCAC CTGGAAAGTC AGACATGGAT 
GCTGCTTTGG ATGACTTAGT AGACACTCTA GGAAAACCTG AAGAAACTAA AGAAGATAAT 
GTACCATATA CTGGACCTGA AATTTCAGAT CCGATGACTT CCACCTACAT AGAGGAATTA 
GGTAAAAGAG AAGTCACACT TCCTCCAAAG TACAGGGAAC TTTTGGCAAA AAAAGAAGGT 
ATCACAGTGC CTCCTCCAGA CTCTTCGAAA CCCATGGGGC CCAATGATGC CATAGATGCT 
TTGTCATCTG ACTTCACCTG CAGTTCACCT ACCGATGCTG GAAAGAAAAC TAAGGAAGAG 
AAATCTACAG GAGAAGAGAT TTTAAAAGCC CAGCCAGCCA GGGTAACCAA AAGTGCTGCT 
TCACCCCAAG AGAAAAAAAG AAAGGTGGAG GAGCAGGACG CAATGAGTGA TCAAGCACTG 
GAGGCTCTTT CAGCTTCTTT GGGCAGCCGG AAGCCAGAAC CCGAGCTGGA CCTCAGTTCC 
ATTAAGGAAG TCGACGAGGC AAAAGCCAAA GAAGAGAAAC TAAAGAAGTG TGGTGAAGAT 
GAGGAAACTG TCCCATCAGA GTACAGATTA AAACCAGCTA CGGATAAAGA TGGAAAACCT 
CTATTGCCAG AGCCTGAAGA AAAATCCAAG CCCCTGAGTG AATCAGAACT CATTGATGAG 
CTTTCAGAAG ATTTTAACCA GCCTAAAAGT AAAGGAGAAC AGTCTAAGCC AACTGAAAAA 
ACAGAAGAAT CTCAGGCTGC CCCCCTAACT CCTGTGAGCG AGGCTGTGCC TCGGACCTCC 
ATGTGTAGCA TACAGTCGGC ACCGCCCAAG CCACCTACCT TGGAAGGCAT GGTGCCAGAT 
GATGCTGTAG AAGCCTTGGC TGGTAGCCTG GGGAAGAAGG AAGCAGATCC AGGAGATGGA 
AAGCCTGTGG AAGATAAAGT CAAGGAGAAA GCCAAGGAAG AGGATCGTGA AAAACTTGGT 
GAAAAAGAAG AAACAATTCC TCCTGATTAT AGATTAGAAG AGGTCAAGGA TAAAGATGGA 
AAACCACTCC TGCCAAAAGT GCCCAAGGAA TCGCTTTTAC CCATGAGTGA AGACTTCCTT 
CTTGATGCTT TGTCCAAGGA CTTTGCTGGT TCCTCAAACA CTTCACCTCT TGAATTTGAA 
GAGGCTAACC TTCCTGCTGT CATCTCTGAA GTGGTTTCCC AAACCCCAGC TCCAACCACG 
CACTCTGCTG GTCTACCCCC TGATACTGTG CAAAGTGACA ACAAAGAACT GGACGATGCT 
TTGGACCAAC TTTCTGACAG TCTGGGACAA AGGCAGCCTG ATCCAGATGA GAACAAGCCG 
ATAGAGGATA AAGTCAAGGA AAGAGCTAAA GCTGAACACA GAGACAAGCT GGGAGAAAGA 
GACGACACCA TCCCCCCTGA ATATAGACAC CTCTTGGATA ACGATGAGGG TAAACCAGGA 
AAGCCACCTG CCAAGAAAAC CAAGGACTCA AAGAAGCCTG AAGATGACAA AGACCCCATC 
GATGCCCTGT CAGGCGACTT CGACAGCTGT CCTGCACCCA CAGAAGCCTC AGAGAACACA 
GCAGAGGACA AAGCCAAGAG AACTGCTCCC AGCTCCAAAA CACCGAAGAA TAGGGGTAAA 
GCAAAGGATT CTGCAAAGGC AAAGCAGGAA ACTTCCAAGC CCAAAGCTGA TGAAAAAAAT 
ACAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_014112203.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013967678.1 Canis lupus familiaris calpastatin (CAST), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OCe35590
Clone ID Related Accession (Same CDS sequence)	XM_014112203.1 , XM_014112203.2
Accession Version	XM_014112203.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	calpastatin isoform X12
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726052.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGCAAAAA CAGAACCTGA AAAGAAGTCA 
CAATCAACTA AGCCAAAAAG CCTACCCAAG CATGCATCAG ATATGGGAAG CAAGCATGTT 
CATAGTGAAA AAGCAGCTTC TAGATCAAGT GAGCAGCTGA CATCAGAGAA ATCAACAAAA 
CCAAAGACTA AAGCACAGGA TTCCGTTTCT GCCGGTGGGG AGAGTGTTGT TGCAGGTGCA 
GCTGCAGCTG CAGCATCTGC CACAGCAGCT AACAAGAAAA AAGAAAAGAA ATCACTAACA 
TCGGCTGTAC CAGTTGAATC CAAACCGGGT AAACCACCTG GAAAGTCAGA CATGGATGCT 
GCTTTGGATG ACTTAGTAGA CACTCTAGGA AAACCTGAAG AAACTAAAGA AGATAATGTA 
CCATATACTG GACCTGAAAT TTCAGATCCG ATGACTTCCA CCTACATAGA GGAATTAGGT 
AAAAGAGAAG TCACACTTCC TCCAAAGTAC AGGGAACTTT TGGCAAAAAA AGAAGGTATC 
ACAGTGCCTC CTCCAGACTC TTCGAAACCC ATGGGGCCCA ATGATGCCAT AGATGCTTTG 
TCATCTGACT TCACCTGCAG TTCACCTACC GATGCTGGAA AGAAAACTAA GGAAGAGAAA 
TCTACAGGAG AAGAGATTTT AAAAGCCCAG CCAGCCAGGG TAACCAAAAG TGCTGCTTCA 
CCCCAAGAGA AAAAAAGAAA GGTGGAGGAG CAGGACGCAA TGAGTGATCA AGCACTGGAG 
GCTCTTTCAG CTTCTTTGGG CAGCCGGAAG CCAGAACCCG AGCTGGACCT CAGTTCCATT 
AAGGAAGTCG ACGAGGCAAA AGCCAAAGAA GAGAAACTAA AGAAGTGTGG TGAAGATGAG 
GAAACTGTCC CATCAGAGTA CAGATTAAAA CCAGCTACGG ATAAAGATGG AAAACCTCTA 
TTGCCAGAGC CTGAAGAAAA ATCCAAGCCC CTGAGTGAAT CAGAACTCAT TGATGAGCTT 
TCAGAAGATT TTAACCAGCC TAAAAGTAAA GGAGAACAGT CTAAGCCAAC TGAAAAAACA 
GAAGAATCTC AGGCTGCCCC CCTAACTCCT GTGAGCGAGG CTGTGCCTCG GACCTCCATG 
TGTAGCATAC AGTCGGCACC GCCCAAGCCA CCTACCTTGG AAGGCATGGT GCCAGATGAT 
GCTGTAGAAG CCTTGGCTGG TAGCCTGGGG AAGAAGGAAG CAGATCCAGG AGATGGAAAG 
CCTGTGGAAG ATAAAGTCAA GGAGAAAGCC AAGGAAGAGG ATCGTGAAAA ACTTGGTGAA 
AAAGAAGAAA CAATTCCTCC TGATTATAGA TTAGAAGAGG TCAAGGATAA AGATGGAAAA 
CCACTCCTGC CAAAAGTGCC CAAGGAATCG CTTTTACCCA TGAGTGAAGA CTTCCTTCTT 
GATGCTTTGT CCAAGGACTT TGCTGGTTCC TCAAACACTT CACCTCTTGA ATTTGAAGAG 
GCTAACCTTC CTGCTGTCAT CTCTGAAGTG GTTTCCCAAA CCCCAGCTCC AACCACGCAC 
TCTGCTGGTC TACCCCCTGA TACTGTGCAA AGTGACAACA AAGAACTGGA CGATGCTTTG 
GACCAACTTT CTGACAGTCT GGGACAAAGG CAGCCTGATC CAGATGAGAA CAAGCCGATA 
GAGGATAAAG TCAAGGAAAG AGCTAAAGCT GAACACAGAG ACAAGCTGGG AGAAAGAGAC 
GACACCATCC CCCCTGAATA TAGACACCTC TTGGATAACG ATGAGGGTAA ACCAGGAAAG 
CCACCTGCCA AGAAAACCAA GGACTCAAAG AAGCCTGAAG ATGACAAAGA CCCCATCGAT 
GCCCTGTCAG GCGACTTCGA CAGCTGTCCT GCACCCACAG AAGCCTCAGA GAACACAGCA 
GAGGACAAAG CCAAGAGAAC TGCTCCCAGC TCCAAAACAC CGAAGAATAG GGGTAAAGCA 
AAGGATTCTG CAAAGGCAAA GCAGGAAACT TCCAAGCCCA AAGCTGATGA AAAAAATACA 
AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967678.1
CDS	102..2387
Translation	MSQPGQKPAASPRPRRAAAARDTQEHVSEKTSGSPSKSGEKKGADEKKARSLESSQPSRTHAGGTAPATKASASSASTSKSSSMNPTETKAAKTEPEKKSQSTKPKSLPKHASDMGSKHVHSEKAASRSSEQLTSEKSTKPKTKAQDSVSAGGESVVAGAAAAAASATAANKKKEKKSLTSAVPVESKPGKPPGKSDMDAALDDLVDTLGKPEETKEDNVPYTGPEISDPMTSTYIEELGKREVTLPPKYRELLAKKEGITVPPPDSSKPMGPNDAIDALSSDFTCSSPTDAGKKTKEEKSTGEEILKAQPARVTKSAASPQEKKRKVEEQDAMSDQALEALSASLGSRKPEPELDLSSIKEVDEAKAKEEKLKKCGEDEETVPSEYRLKPATDKDGKPLLPEPEEKSKPLSESELIDELSEDFNQPKSKGEQSKPTEKTEESQAAPLTPVSEAVPRTSMCSIQSAPPKPPTLEGMVPDDAVEALAGSLGKKEADPGDGKPVEDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKVPKESLLPMSEDFLLDALSKDFAGSSNTSPLEFEEANLPAVISEVVSQTPAPTTHSAGLPPDTVQSDNKELDDALDQLSDSLGQRQPDPDENKPIEDKVKERAKAEHRDKLGERDDTIPPEYRHLLDNDEGKPGKPPAKKTKDSKKPEDDKDPIDALSGDFDSCPAPTEASENTAEDKAKRTAPSSKTPKNRGKAKDSAKAKQETSKPKADEKNTS

Target ORF information:

RefSeq Version	XM_014112203.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calpastatin (CAST), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014112203.1

ORF Insert Sequence:

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGCAAAAA CAGAACCTGA AAAGAAGTCA 
CAATCAACTA AGCCAAAAAG CCTACCCAAG CATGCATCAG ATATGGGAAG CAAGCATGTT 
CATAGTGAAA AAGCAGCTTC TAGATCAAGT GAGCAGCTGA CATCAGAGAA ATCAACAAAA 
CCAAAGACTA AAGCACAGGA TTCCGTTTCT GCCGGTGGGG AGAGTGTTGT TGCAGGTGCA 
GCTGCAGCTG CAGCATCTGC CACAGCAGCT AACAAGAAAA AAGAAAAGAA ATCACTAACA 
TCGGCTGTAC CAGTTGAATC CAAACCGGGT AAACCACCTG GAAAGTCAGA CATGGATGCT 
GCTTTGGATG ACTTAGTAGA CACTCTAGGA AAACCTGAAG AAACTAAAGA AGATAATGTA 
CCATATACTG GACCTGAAAT TTCAGATCCG ATGACTTCCA CCTACATAGA GGAATTAGGT 
AAAAGAGAAG TCACACTTCC TCCAAAGTAC AGGGAACTTT TGGCAAAAAA AGAAGGTATC 
ACAGTGCCTC CTCCAGACTC TTCGAAACCC ATGGGGCCCA ATGATGCCAT AGATGCTTTG 
TCATCTGACT TCACCTGCAG TTCACCTACC GATGCTGGAA AGAAAACTAA GGAAGAGAAA 
TCTACAGGAG AAGAGATTTT AAAAGCCCAG CCAGCCAGGG TAACCAAAAG TGCTGCTTCA 
CCCCAAGAGA AAAAAAGAAA GGTGGAGGAG CAGGACGCAA TGAGTGATCA AGCACTGGAG 
GCTCTTTCAG CTTCTTTGGG CAGCCGGAAG CCAGAACCCG AGCTGGACCT CAGTTCCATT 
AAGGAAGTCG ACGAGGCAAA AGCCAAAGAA GAGAAACTAA AGAAGTGTGG TGAAGATGAG 
GAAACTGTCC CATCAGAGTA CAGATTAAAA CCAGCTACGG ATAAAGATGG AAAACCTCTA 
TTGCCAGAGC CTGAAGAAAA ATCCAAGCCC CTGAGTGAAT CAGAACTCAT TGATGAGCTT 
TCAGAAGATT TTAACCAGCC TAAAAGTAAA GGAGAACAGT CTAAGCCAAC TGAAAAAACA 
GAAGAATCTC AGGCTGCCCC CCTAACTCCT GTGAGCGAGG CTGTGCCTCG GACCTCCATG 
TGTAGCATAC AGTCGGCACC GCCCAAGCCA CCTACCTTGG AAGGCATGGT GCCAGATGAT 
GCTGTAGAAG CCTTGGCTGG TAGCCTGGGG AAGAAGGAAG CAGATCCAGG AGATGGAAAG 
CCTGTGGAAG ATAAAGTCAA GGAGAAAGCC AAGGAAGAGG ATCGTGAAAA ACTTGGTGAA 
AAAGAAGAAA CAATTCCTCC TGATTATAGA TTAGAAGAGG TCAAGGATAA AGATGGAAAA 
CCACTCCTGC CAAAAGTGCC CAAGGAATCG CTTTTACCCA TGAGTGAAGA CTTCCTTCTT 
GATGCTTTGT CCAAGGACTT TGCTGGTTCC TCAAACACTT CACCTCTTGA ATTTGAAGAG 
GCTAACCTTC CTGCTGTCAT CTCTGAAGTG GTTTCCCAAA CCCCAGCTCC AACCACGCAC 
TCTGCTGGTC TACCCCCTGA TACTGTGCAA AGTGACAACA AAGAACTGGA CGATGCTTTG 
GACCAACTTT CTGACAGTCT GGGACAAAGG CAGCCTGATC CAGATGAGAA CAAGCCGATA 
GAGGATAAAG TCAAGGAAAG AGCTAAAGCT GAACACAGAG ACAAGCTGGG AGAAAGAGAC 
GACACCATCC CCCCTGAATA TAGACACCTC TTGGATAACG ATGAGGGTAA ACCAGGAAAG 
CCACCTGCCA AGAAAACCAA GGACTCAAAG AAGCCTGAAG ATGACAAAGA CCCCATCGAT 
GCCCTGTCAG GCGACTTCGA CAGCTGTCCT GCACCCACAG AAGCCTCAGA GAACACAGCA 
GAGGACAAAG CCAAGAGAAC TGCTCCCAGC TCCAAAACAC CGAAGAATAG GGGTAAAGCA 
AAGGATTCTG CAAAGGCAAA GCAGGAAACT TCCAAGCCCA AAGCTGATGA AAAAAATACA 
AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_014112203.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013967678.1 Canis lupus familiaris calpastatin (CAST), transcript variant X15, mRNA.

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CloneID	OCe35590
Clone ID Related Accession (Same CDS sequence)	XM_014112203.1 , XM_014112203.2
Accession Version	XM_014112203.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	calpastatin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014112203.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGCAAAAA CAGAACCTGA AAAGAAGTCA 
CAATCAACTA AGCCAAAAAG CCTACCCAAG CATGCATCAG ATATGGGAAG CAAGCATGTT 
CATAGTGAAA AAGCAGCTTC TAGATCAAGT GAGCAGCTGA CATCAGAGAA ATCAACAAAA 
CCAAAGACTA AAGCACAGGA TTCCGTTTCT GCCGGTGGGG AGAGTGTTGT TGCAGGTGCA 
GCTGCAGCTG CAGCATCTGC CACAGCAGCT AACAAGAAAA AAGAAAAGAA ATCACTAACA 
TCGGCTGTAC CAGTTGAATC CAAACCGGGT AAACCACCTG GAAAGTCAGA CATGGATGCT 
GCTTTGGATG ACTTAGTAGA CACTCTAGGA AAACCTGAAG AAACTAAAGA AGATAATGTA 
CCATATACTG GACCTGAAAT TTCAGATCCG ATGACTTCCA CCTACATAGA GGAATTAGGT 
AAAAGAGAAG TCACACTTCC TCCAAAGTAC AGGGAACTTT TGGCAAAAAA AGAAGGTATC 
ACAGTGCCTC CTCCAGACTC TTCGAAACCC ATGGGGCCCA ATGATGCCAT AGATGCTTTG 
TCATCTGACT TCACCTGCAG TTCACCTACC GATGCTGGAA AGAAAACTAA GGAAGAGAAA 
TCTACAGGAG AAGAGATTTT AAAAGCCCAG CCAGCCAGGG TAACCAAAAG TGCTGCTTCA 
CCCCAAGAGA AAAAAAGAAA GGTGGAGGAG CAGGACGCAA TGAGTGATCA AGCACTGGAG 
GCTCTTTCAG CTTCTTTGGG CAGCCGGAAG CCAGAACCCG AGCTGGACCT CAGTTCCATT 
AAGGAAGTCG ACGAGGCAAA AGCCAAAGAA GAGAAACTAA AGAAGTGTGG TGAAGATGAG 
GAAACTGTCC CATCAGAGTA CAGATTAAAA CCAGCTACGG ATAAAGATGG AAAACCTCTA 
TTGCCAGAGC CTGAAGAAAA ATCCAAGCCC CTGAGTGAAT CAGAACTCAT TGATGAGCTT 
TCAGAAGATT TTAACCAGCC TAAAAGTAAA GGAGAACAGT CTAAGCCAAC TGAAAAAACA 
GAAGAATCTC AGGCTGCCCC CCTAACTCCT GTGAGCGAGG CTGTGCCTCG GACCTCCATG 
TGTAGCATAC AGTCGGCACC GCCCAAGCCA CCTACCTTGG AAGGCATGGT GCCAGATGAT 
GCTGTAGAAG CCTTGGCTGG TAGCCTGGGG AAGAAGGAAG CAGATCCAGG AGATGGAAAG 
CCTGTGGAAG ATAAAGTCAA GGAGAAAGCC AAGGAAGAGG ATCGTGAAAA ACTTGGTGAA 
AAAGAAGAAA CAATTCCTCC TGATTATAGA TTAGAAGAGG TCAAGGATAA AGATGGAAAA 
CCACTCCTGC CAAAAGTGCC CAAGGAATCG CTTTTACCCA TGAGTGAAGA CTTCCTTCTT 
GATGCTTTGT CCAAGGACTT TGCTGGTTCC TCAAACACTT CACCTCTTGA ATTTGAAGAG 
GCTAACCTTC CTGCTGTCAT CTCTGAAGTG GTTTCCCAAA CCCCAGCTCC AACCACGCAC 
TCTGCTGGTC TACCCCCTGA TACTGTGCAA AGTGACAACA AAGAACTGGA CGATGCTTTG 
GACCAACTTT CTGACAGTCT GGGACAAAGG CAGCCTGATC CAGATGAGAA CAAGCCGATA 
GAGGATAAAG TCAAGGAAAG AGCTAAAGCT GAACACAGAG ACAAGCTGGG AGAAAGAGAC 
GACACCATCC CCCCTGAATA TAGACACCTC TTGGATAACG ATGAGGGTAA ACCAGGAAAG 
CCACCTGCCA AGAAAACCAA GGACTCAAAG AAGCCTGAAG ATGACAAAGA CCCCATCGAT 
GCCCTGTCAG GCGACTTCGA CAGCTGTCCT GCACCCACAG AAGCCTCAGA GAACACAGCA 
GAGGACAAAG CCAAGAGAAC TGCTCCCAGC TCCAAAACAC CGAAGAATAG GGGTAAAGCA 
AAGGATTCTG CAAAGGCAAA GCAGGAAACT TCCAAGCCCA AAGCTGATGA AAAAAATACA 
AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967678.1
CDS	79..2364
Translation	MSQPGQKPAASPRPRRAAAARDTQEHVSEKTSGSPSKSGEKKGADEKKARSLESSQPSRTHAGGTAPATKASASSASTSKSSSMNPTETKAAKTEPEKKSQSTKPKSLPKHASDMGSKHVHSEKAASRSSEQLTSEKSTKPKTKAQDSVSAGGESVVAGAAAAAASATAANKKKEKKSLTSAVPVESKPGKPPGKSDMDAALDDLVDTLGKPEETKEDNVPYTGPEISDPMTSTYIEELGKREVTLPPKYRELLAKKEGITVPPPDSSKPMGPNDAIDALSSDFTCSSPTDAGKKTKEEKSTGEEILKAQPARVTKSAASPQEKKRKVEEQDAMSDQALEALSASLGSRKPEPELDLSSIKEVDEAKAKEEKLKKCGEDEETVPSEYRLKPATDKDGKPLLPEPEEKSKPLSESELIDELSEDFNQPKSKGEQSKPTEKTEESQAAPLTPVSEAVPRTSMCSIQSAPPKPPTLEGMVPDDAVEALAGSLGKKEADPGDGKPVEDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKVPKESLLPMSEDFLLDALSKDFAGSSNTSPLEFEEANLPAVISEVVSQTPAPTTHSAGLPPDTVQSDNKELDDALDQLSDSLGQRQPDPDENKPIEDKVKERAKAEHRDKLGERDDTIPPEYRHLLDNDEGKPGKPPAKKTKDSKKPEDDKDPIDALSGDFDSCPAPTEASENTAEDKAKRTAPSSKTPKNRGKAKDSAKAKQETSKPKADEKNTS

Target ORF information:

RefSeq Version	XM_014112203.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calpastatin (CAST), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014112203.2

ORF Insert Sequence:

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGCAAAAA CAGAACCTGA AAAGAAGTCA 
CAATCAACTA AGCCAAAAAG CCTACCCAAG CATGCATCAG ATATGGGAAG CAAGCATGTT 
CATAGTGAAA AAGCAGCTTC TAGATCAAGT GAGCAGCTGA CATCAGAGAA ATCAACAAAA 
CCAAAGACTA AAGCACAGGA TTCCGTTTCT GCCGGTGGGG AGAGTGTTGT TGCAGGTGCA 
GCTGCAGCTG CAGCATCTGC CACAGCAGCT AACAAGAAAA AAGAAAAGAA ATCACTAACA 
TCGGCTGTAC CAGTTGAATC CAAACCGGGT AAACCACCTG GAAAGTCAGA CATGGATGCT 
GCTTTGGATG ACTTAGTAGA CACTCTAGGA AAACCTGAAG AAACTAAAGA AGATAATGTA 
CCATATACTG GACCTGAAAT TTCAGATCCG ATGACTTCCA CCTACATAGA GGAATTAGGT 
AAAAGAGAAG TCACACTTCC TCCAAAGTAC AGGGAACTTT TGGCAAAAAA AGAAGGTATC 
ACAGTGCCTC CTCCAGACTC TTCGAAACCC ATGGGGCCCA ATGATGCCAT AGATGCTTTG 
TCATCTGACT TCACCTGCAG TTCACCTACC GATGCTGGAA AGAAAACTAA GGAAGAGAAA 
TCTACAGGAG AAGAGATTTT AAAAGCCCAG CCAGCCAGGG TAACCAAAAG TGCTGCTTCA 
CCCCAAGAGA AAAAAAGAAA GGTGGAGGAG CAGGACGCAA TGAGTGATCA AGCACTGGAG 
GCTCTTTCAG CTTCTTTGGG CAGCCGGAAG CCAGAACCCG AGCTGGACCT CAGTTCCATT 
AAGGAAGTCG ACGAGGCAAA AGCCAAAGAA GAGAAACTAA AGAAGTGTGG TGAAGATGAG 
GAAACTGTCC CATCAGAGTA CAGATTAAAA CCAGCTACGG ATAAAGATGG AAAACCTCTA 
TTGCCAGAGC CTGAAGAAAA ATCCAAGCCC CTGAGTGAAT CAGAACTCAT TGATGAGCTT 
TCAGAAGATT TTAACCAGCC TAAAAGTAAA GGAGAACAGT CTAAGCCAAC TGAAAAAACA 
GAAGAATCTC AGGCTGCCCC CCTAACTCCT GTGAGCGAGG CTGTGCCTCG GACCTCCATG 
TGTAGCATAC AGTCGGCACC GCCCAAGCCA CCTACCTTGG AAGGCATGGT GCCAGATGAT 
GCTGTAGAAG CCTTGGCTGG TAGCCTGGGG AAGAAGGAAG CAGATCCAGG AGATGGAAAG 
CCTGTGGAAG ATAAAGTCAA GGAGAAAGCC AAGGAAGAGG ATCGTGAAAA ACTTGGTGAA 
AAAGAAGAAA CAATTCCTCC TGATTATAGA TTAGAAGAGG TCAAGGATAA AGATGGAAAA 
CCACTCCTGC CAAAAGTGCC CAAGGAATCG CTTTTACCCA TGAGTGAAGA CTTCCTTCTT 
GATGCTTTGT CCAAGGACTT TGCTGGTTCC TCAAACACTT CACCTCTTGA ATTTGAAGAG 
GCTAACCTTC CTGCTGTCAT CTCTGAAGTG GTTTCCCAAA CCCCAGCTCC AACCACGCAC 
TCTGCTGGTC TACCCCCTGA TACTGTGCAA AGTGACAACA AAGAACTGGA CGATGCTTTG 
GACCAACTTT CTGACAGTCT GGGACAAAGG CAGCCTGATC CAGATGAGAA CAAGCCGATA 
GAGGATAAAG TCAAGGAAAG AGCTAAAGCT GAACACAGAG ACAAGCTGGG AGAAAGAGAC 
GACACCATCC CCCCTGAATA TAGACACCTC TTGGATAACG ATGAGGGTAA ACCAGGAAAG 
CCACCTGCCA AGAAAACCAA GGACTCAAAG AAGCCTGAAG ATGACAAAGA CCCCATCGAT 
GCCCTGTCAG GCGACTTCGA CAGCTGTCCT GCACCCACAG AAGCCTCAGA GAACACAGCA 
GAGGACAAAG CCAAGAGAAC TGCTCCCAGC TCCAAAACAC CGAAGAATAG GGGTAAAGCA 
AAGGATTCTG CAAAGGCAAA GCAGGAAACT TCCAAGCCCA AAGCTGATGA AAAAAATACA 
AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_014112204.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013967679.1 Canis lupus familiaris calpastatin (CAST), transcript variant X13, mRNA.

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CloneID	OCe35591
Clone ID Related Accession (Same CDS sequence)	XM_014112204.1 , XM_014112204.2
Accession Version	XM_014112204.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	calpastatin isoform X13
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726052.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGCAAAAA CAGAACCTGA AAAGAAGTCA 
CAATCAACTA AGCCAAAAAG CCTACCCAAG CATGCATCAG ATATGGGAAG CAAGCATGTT 
CATAGTGAAA AAGCAGCTTC TAGATCAAGT GAGCAGCTGA CATCAGAGAA ATCAACAAAA 
CCAAAGACTA AAGCACAGGA TTCCGTTTCT GCCGGTGGGG AGAGTGTTGT TGCAGGTGCA 
GCTGCAGCTG CAGCATCTGC CACAGCAGCT AACAAGAAAA AAGAAAAGAA ATCACTAACA 
TCGGCTGTAC CAGTTGAATC CAAACCGGGT AAACCACCTG GAAAGTCAGA CATGGATGCT 
GCTTTGGATG ACTTAGTAGA CACTCTAGGA AAACCTGAAG AAACTAAAGA AGATAATGTA 
CCATATACTG GACCTGAAAT TTCAGATCCG ATGACTTCCA CCTACATAGA GGAATTAGGT 
AAAAGAGAAG TCACACTTCC TCCAAAGTAC AGGGAACTTT TGGCAAAAAA AGAAGGTATC 
ACAGTGCCTC CTCCAGACTC TTCGAAACCC ATGGGGCCCA ATGATGCCAT AGATGCTTTG 
TCATCTGACT TCACCTGCAG TTCACCTACC GATGCTGGAA AGAAAACTAA GGAAGAGAAA 
TCTACAGGAG AAGAGATTTT AAAAGCCCAG CCAGCCAGGG TAACCAAAAG TGCTGCTTCA 
CCCCAAGAGA AAAAAAGAAA GGTGGAGGAG GACGCAATGA GTGATCAAGC ACTGGAGGCT 
CTTTCAGCTT CTTTGGGCAG CCGGAAGCCA GAACCCGAGC TGGACCTCAG TTCCATTAAG 
GAAGTCGACG AGGCAAAAGC CAAAGAAGAG AAACTAAAGA AGTGTGGTGA AGATGAGGAA 
ACTGTCCCAT CAGAGTACAG ATTAAAACCA GCTACGGATA AAGATGGAAA ACCTCTATTG 
CCAGAGCCTG AAGAAAAATC CAAGCCCCTG AGTGAATCAG AACTCATTGA TGAGCTTTCA 
GAAGATTTTA ACCAGCCTAA AAGTAAAGGA GAACAGTCTA AGCCAACTGA AAAAACAGAA 
GAATCTCAGG CTGCCCCCCT AACTCCTGTG AGCGAGGCTG TGCCTCGGAC CTCCATGTGT 
AGCATACAGT CGGCACCGCC CAAGCCACCT ACCTTGGAAG GCATGGTGCC AGATGATGCT 
GTAGAAGCCT TGGCTGGTAG CCTGGGGAAG AAGGAAGCAG ATCCAGGAGA TGGAAAGCCT 
GTGGAAGATA AAGTCAAGGA GAAAGCCAAG GAAGAGGATC GTGAAAAACT TGGTGAAAAA 
GAAGAAACAA TTCCTCCTGA TTATAGATTA GAAGAGGTCA AGGATAAAGA TGGAAAACCA 
CTCCTGCCAA AAGTGCCCAA GGAATCGCTT TTACCCATGA GTGAAGACTT CCTTCTTGAT 
GCTTTGTCCA AGGACTTTGC TGGTTCCTCA AACACTTCAC CTCTTGAATT TGAAGAGGCT 
AACCTTCCTG CTGTCATCTC TGAAGTGGTT TCCCAAACCC CAGCTCCAAC CACGCACTCT 
GCTGGTCTAC CCCCTGATAC TGTGCAAAGT GACAACAAAG AACTGGACGA TGCTTTGGAC 
CAACTTTCTG ACAGTCTGGG ACAAAGGCAG CCTGATCCAG ATGAGAACAA GCCGATAGAG 
GATAAAGTCA AGGAAAGAGC TAAAGCTGAA CACAGAGACA AGCTGGGAGA AAGAGACGAC 
ACCATCCCCC CTGAATATAG ACACCTCTTG GATAACGATG AGGGTAAACC AGGAAAGCCA 
CCTGCCAAGA AAACCAAGGA CTCAAAGAAG CCTGAAGATG ACAAAGACCC CATCGATGCC 
CTGTCAGGCG ACTTCGACAG CTGTCCTGCA CCCACAGAAG CCTCAGAGAA CACAGCAGAG 
GACAAAGCCA AGAGAACTGC TCCCAGCTCC AAAACACCGA AGAATAGGGG TAAAGCAAAG 
GATTCTGCAA AGGCAAAGCA GGAAACTTCC AAGCCCAAAG CTGATGAAAA AAATACAAGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967679.1
CDS	102..2384
Translation	MSQPGQKPAASPRPRRAAAARDTQEHVSEKTSGSPSKSGEKKGADEKKARSLESSQPSRTHAGGTAPATKASASSASTSKSSSMNPTETKAAKTEPEKKSQSTKPKSLPKHASDMGSKHVHSEKAASRSSEQLTSEKSTKPKTKAQDSVSAGGESVVAGAAAAAASATAANKKKEKKSLTSAVPVESKPGKPPGKSDMDAALDDLVDTLGKPEETKEDNVPYTGPEISDPMTSTYIEELGKREVTLPPKYRELLAKKEGITVPPPDSSKPMGPNDAIDALSSDFTCSSPTDAGKKTKEEKSTGEEILKAQPARVTKSAASPQEKKRKVEEDAMSDQALEALSASLGSRKPEPELDLSSIKEVDEAKAKEEKLKKCGEDEETVPSEYRLKPATDKDGKPLLPEPEEKSKPLSESELIDELSEDFNQPKSKGEQSKPTEKTEESQAAPLTPVSEAVPRTSMCSIQSAPPKPPTLEGMVPDDAVEALAGSLGKKEADPGDGKPVEDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKVPKESLLPMSEDFLLDALSKDFAGSSNTSPLEFEEANLPAVISEVVSQTPAPTTHSAGLPPDTVQSDNKELDDALDQLSDSLGQRQPDPDENKPIEDKVKERAKAEHRDKLGERDDTIPPEYRHLLDNDEGKPGKPPAKKTKDSKKPEDDKDPIDALSGDFDSCPAPTEASENTAEDKAKRTAPSSKTPKNRGKAKDSAKAKQETSKPKADEKNTS

Target ORF information:

RefSeq Version	XM_014112204.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calpastatin (CAST), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014112204.1

ORF Insert Sequence:

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGCAAAAA CAGAACCTGA AAAGAAGTCA 
CAATCAACTA AGCCAAAAAG CCTACCCAAG CATGCATCAG ATATGGGAAG CAAGCATGTT 
CATAGTGAAA AAGCAGCTTC TAGATCAAGT GAGCAGCTGA CATCAGAGAA ATCAACAAAA 
CCAAAGACTA AAGCACAGGA TTCCGTTTCT GCCGGTGGGG AGAGTGTTGT TGCAGGTGCA 
GCTGCAGCTG CAGCATCTGC CACAGCAGCT AACAAGAAAA AAGAAAAGAA ATCACTAACA 
TCGGCTGTAC CAGTTGAATC CAAACCGGGT AAACCACCTG GAAAGTCAGA CATGGATGCT 
GCTTTGGATG ACTTAGTAGA CACTCTAGGA AAACCTGAAG AAACTAAAGA AGATAATGTA 
CCATATACTG GACCTGAAAT TTCAGATCCG ATGACTTCCA CCTACATAGA GGAATTAGGT 
AAAAGAGAAG TCACACTTCC TCCAAAGTAC AGGGAACTTT TGGCAAAAAA AGAAGGTATC 
ACAGTGCCTC CTCCAGACTC TTCGAAACCC ATGGGGCCCA ATGATGCCAT AGATGCTTTG 
TCATCTGACT TCACCTGCAG TTCACCTACC GATGCTGGAA AGAAAACTAA GGAAGAGAAA 
TCTACAGGAG AAGAGATTTT AAAAGCCCAG CCAGCCAGGG TAACCAAAAG TGCTGCTTCA 
CCCCAAGAGA AAAAAAGAAA GGTGGAGGAG GACGCAATGA GTGATCAAGC ACTGGAGGCT 
CTTTCAGCTT CTTTGGGCAG CCGGAAGCCA GAACCCGAGC TGGACCTCAG TTCCATTAAG 
GAAGTCGACG AGGCAAAAGC CAAAGAAGAG AAACTAAAGA AGTGTGGTGA AGATGAGGAA 
ACTGTCCCAT CAGAGTACAG ATTAAAACCA GCTACGGATA AAGATGGAAA ACCTCTATTG 
CCAGAGCCTG AAGAAAAATC CAAGCCCCTG AGTGAATCAG AACTCATTGA TGAGCTTTCA 
GAAGATTTTA ACCAGCCTAA AAGTAAAGGA GAACAGTCTA AGCCAACTGA AAAAACAGAA 
GAATCTCAGG CTGCCCCCCT AACTCCTGTG AGCGAGGCTG TGCCTCGGAC CTCCATGTGT 
AGCATACAGT CGGCACCGCC CAAGCCACCT ACCTTGGAAG GCATGGTGCC AGATGATGCT 
GTAGAAGCCT TGGCTGGTAG CCTGGGGAAG AAGGAAGCAG ATCCAGGAGA TGGAAAGCCT 
GTGGAAGATA AAGTCAAGGA GAAAGCCAAG GAAGAGGATC GTGAAAAACT TGGTGAAAAA 
GAAGAAACAA TTCCTCCTGA TTATAGATTA GAAGAGGTCA AGGATAAAGA TGGAAAACCA 
CTCCTGCCAA AAGTGCCCAA GGAATCGCTT TTACCCATGA GTGAAGACTT CCTTCTTGAT 
GCTTTGTCCA AGGACTTTGC TGGTTCCTCA AACACTTCAC CTCTTGAATT TGAAGAGGCT 
AACCTTCCTG CTGTCATCTC TGAAGTGGTT TCCCAAACCC CAGCTCCAAC CACGCACTCT 
GCTGGTCTAC CCCCTGATAC TGTGCAAAGT GACAACAAAG AACTGGACGA TGCTTTGGAC 
CAACTTTCTG ACAGTCTGGG ACAAAGGCAG CCTGATCCAG ATGAGAACAA GCCGATAGAG 
GATAAAGTCA AGGAAAGAGC TAAAGCTGAA CACAGAGACA AGCTGGGAGA AAGAGACGAC 
ACCATCCCCC CTGAATATAG ACACCTCTTG GATAACGATG AGGGTAAACC AGGAAAGCCA 
CCTGCCAAGA AAACCAAGGA CTCAAAGAAG CCTGAAGATG ACAAAGACCC CATCGATGCC 
CTGTCAGGCG ACTTCGACAG CTGTCCTGCA CCCACAGAAG CCTCAGAGAA CACAGCAGAG 
GACAAAGCCA AGAGAACTGC TCCCAGCTCC AAAACACCGA AGAATAGGGG TAAAGCAAAG 
GATTCTGCAA AGGCAAAGCA GGAAACTTCC AAGCCCAAAG CTGATGAAAA AAATACAAGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAST ( XM_014112204.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013967679.1 Canis lupus familiaris calpastatin (CAST), transcript variant X16, mRNA.

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CloneID	OCe35591
Clone ID Related Accession (Same CDS sequence)	XM_014112204.1 , XM_014112204.2
Accession Version	XM_014112204.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	calpastatin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014112204.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGCAAAAA CAGAACCTGA AAAGAAGTCA 
CAATCAACTA AGCCAAAAAG CCTACCCAAG CATGCATCAG ATATGGGAAG CAAGCATGTT 
CATAGTGAAA AAGCAGCTTC TAGATCAAGT GAGCAGCTGA CATCAGAGAA ATCAACAAAA 
CCAAAGACTA AAGCACAGGA TTCCGTTTCT GCCGGTGGGG AGAGTGTTGT TGCAGGTGCA 
GCTGCAGCTG CAGCATCTGC CACAGCAGCT AACAAGAAAA AAGAAAAGAA ATCACTAACA 
TCGGCTGTAC CAGTTGAATC CAAACCGGGT AAACCACCTG GAAAGTCAGA CATGGATGCT 
GCTTTGGATG ACTTAGTAGA CACTCTAGGA AAACCTGAAG AAACTAAAGA AGATAATGTA 
CCATATACTG GACCTGAAAT TTCAGATCCG ATGACTTCCA CCTACATAGA GGAATTAGGT 
AAAAGAGAAG TCACACTTCC TCCAAAGTAC AGGGAACTTT TGGCAAAAAA AGAAGGTATC 
ACAGTGCCTC CTCCAGACTC TTCGAAACCC ATGGGGCCCA ATGATGCCAT AGATGCTTTG 
TCATCTGACT TCACCTGCAG TTCACCTACC GATGCTGGAA AGAAAACTAA GGAAGAGAAA 
TCTACAGGAG AAGAGATTTT AAAAGCCCAG CCAGCCAGGG TAACCAAAAG TGCTGCTTCA 
CCCCAAGAGA AAAAAAGAAA GGTGGAGGAG GACGCAATGA GTGATCAAGC ACTGGAGGCT 
CTTTCAGCTT CTTTGGGCAG CCGGAAGCCA GAACCCGAGC TGGACCTCAG TTCCATTAAG 
GAAGTCGACG AGGCAAAAGC CAAAGAAGAG AAACTAAAGA AGTGTGGTGA AGATGAGGAA 
ACTGTCCCAT CAGAGTACAG ATTAAAACCA GCTACGGATA AAGATGGAAA ACCTCTATTG 
CCAGAGCCTG AAGAAAAATC CAAGCCCCTG AGTGAATCAG AACTCATTGA TGAGCTTTCA 
GAAGATTTTA ACCAGCCTAA AAGTAAAGGA GAACAGTCTA AGCCAACTGA AAAAACAGAA 
GAATCTCAGG CTGCCCCCCT AACTCCTGTG AGCGAGGCTG TGCCTCGGAC CTCCATGTGT 
AGCATACAGT CGGCACCGCC CAAGCCACCT ACCTTGGAAG GCATGGTGCC AGATGATGCT 
GTAGAAGCCT TGGCTGGTAG CCTGGGGAAG AAGGAAGCAG ATCCAGGAGA TGGAAAGCCT 
GTGGAAGATA AAGTCAAGGA GAAAGCCAAG GAAGAGGATC GTGAAAAACT TGGTGAAAAA 
GAAGAAACAA TTCCTCCTGA TTATAGATTA GAAGAGGTCA AGGATAAAGA TGGAAAACCA 
CTCCTGCCAA AAGTGCCCAA GGAATCGCTT TTACCCATGA GTGAAGACTT CCTTCTTGAT 
GCTTTGTCCA AGGACTTTGC TGGTTCCTCA AACACTTCAC CTCTTGAATT TGAAGAGGCT 
AACCTTCCTG CTGTCATCTC TGAAGTGGTT TCCCAAACCC CAGCTCCAAC CACGCACTCT 
GCTGGTCTAC CCCCTGATAC TGTGCAAAGT GACAACAAAG AACTGGACGA TGCTTTGGAC 
CAACTTTCTG ACAGTCTGGG ACAAAGGCAG CCTGATCCAG ATGAGAACAA GCCGATAGAG 
GATAAAGTCA AGGAAAGAGC TAAAGCTGAA CACAGAGACA AGCTGGGAGA AAGAGACGAC 
ACCATCCCCC CTGAATATAG ACACCTCTTG GATAACGATG AGGGTAAACC AGGAAAGCCA 
CCTGCCAAGA AAACCAAGGA CTCAAAGAAG CCTGAAGATG ACAAAGACCC CATCGATGCC 
CTGTCAGGCG ACTTCGACAG CTGTCCTGCA CCCACAGAAG CCTCAGAGAA CACAGCAGAG 
GACAAAGCCA AGAGAACTGC TCCCAGCTCC AAAACACCGA AGAATAGGGG TAAAGCAAAG 
GATTCTGCAA AGGCAAAGCA GGAAACTTCC AAGCCCAAAG CTGATGAAAA AAATACAAGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967679.1
CDS	79..2361
Translation	MSQPGQKPAASPRPRRAAAARDTQEHVSEKTSGSPSKSGEKKGADEKKARSLESSQPSRTHAGGTAPATKASASSASTSKSSSMNPTETKAAKTEPEKKSQSTKPKSLPKHASDMGSKHVHSEKAASRSSEQLTSEKSTKPKTKAQDSVSAGGESVVAGAAAAAASATAANKKKEKKSLTSAVPVESKPGKPPGKSDMDAALDDLVDTLGKPEETKEDNVPYTGPEISDPMTSTYIEELGKREVTLPPKYRELLAKKEGITVPPPDSSKPMGPNDAIDALSSDFTCSSPTDAGKKTKEEKSTGEEILKAQPARVTKSAASPQEKKRKVEEDAMSDQALEALSASLGSRKPEPELDLSSIKEVDEAKAKEEKLKKCGEDEETVPSEYRLKPATDKDGKPLLPEPEEKSKPLSESELIDELSEDFNQPKSKGEQSKPTEKTEESQAAPLTPVSEAVPRTSMCSIQSAPPKPPTLEGMVPDDAVEALAGSLGKKEADPGDGKPVEDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKVPKESLLPMSEDFLLDALSKDFAGSSNTSPLEFEEANLPAVISEVVSQTPAPTTHSAGLPPDTVQSDNKELDDALDQLSDSLGQRQPDPDENKPIEDKVKERAKAEHRDKLGERDDTIPPEYRHLLDNDEGKPGKPPAKKTKDSKKPEDDKDPIDALSGDFDSCPAPTEASENTAEDKAKRTAPSSKTPKNRGKAKDSAKAKQETSKPKADEKNTS

Target ORF information:

RefSeq Version	XM_014112204.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calpastatin (CAST), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014112204.2

ORF Insert Sequence:

ATGTCTCAGC CGGGCCAGAA GCCCGCTGCC TCCCCGAGGC CCCGGCGCGC TGCCGCCGCC 
CGCGACACCC AGGAGCATGT CAGCGAAAAA ACAAGTGGAT CACCTTCCAA GTCAGGAGAA 
AAGAAAGGAG CAGATGAGAA AAAAGCAAGA AGCCTTGAGA GCAGTCAACC TTCCAGAACC 
CATGCTGGTG GAACAGCCCC AGCCACCAAG GCGTCAGCCT CTTCTGCTTC TACCAGCAAG 
TCTTCCAGTA TGAATCCCAC AGAAACCAAG GCTGCAAAAA CAGAACCTGA AAAGAAGTCA 
CAATCAACTA AGCCAAAAAG CCTACCCAAG CATGCATCAG ATATGGGAAG CAAGCATGTT 
CATAGTGAAA AAGCAGCTTC TAGATCAAGT GAGCAGCTGA CATCAGAGAA ATCAACAAAA 
CCAAAGACTA AAGCACAGGA TTCCGTTTCT GCCGGTGGGG AGAGTGTTGT TGCAGGTGCA 
GCTGCAGCTG CAGCATCTGC CACAGCAGCT AACAAGAAAA AAGAAAAGAA ATCACTAACA 
TCGGCTGTAC CAGTTGAATC CAAACCGGGT AAACCACCTG GAAAGTCAGA CATGGATGCT 
GCTTTGGATG ACTTAGTAGA CACTCTAGGA AAACCTGAAG AAACTAAAGA AGATAATGTA 
CCATATACTG GACCTGAAAT TTCAGATCCG ATGACTTCCA CCTACATAGA GGAATTAGGT 
AAAAGAGAAG TCACACTTCC TCCAAAGTAC AGGGAACTTT TGGCAAAAAA AGAAGGTATC 
ACAGTGCCTC CTCCAGACTC TTCGAAACCC ATGGGGCCCA ATGATGCCAT AGATGCTTTG 
TCATCTGACT TCACCTGCAG TTCACCTACC GATGCTGGAA AGAAAACTAA GGAAGAGAAA 
TCTACAGGAG AAGAGATTTT AAAAGCCCAG CCAGCCAGGG TAACCAAAAG TGCTGCTTCA 
CCCCAAGAGA AAAAAAGAAA GGTGGAGGAG GACGCAATGA GTGATCAAGC ACTGGAGGCT 
CTTTCAGCTT CTTTGGGCAG CCGGAAGCCA GAACCCGAGC TGGACCTCAG TTCCATTAAG 
GAAGTCGACG AGGCAAAAGC CAAAGAAGAG AAACTAAAGA AGTGTGGTGA AGATGAGGAA 
ACTGTCCCAT CAGAGTACAG ATTAAAACCA GCTACGGATA AAGATGGAAA ACCTCTATTG 
CCAGAGCCTG AAGAAAAATC CAAGCCCCTG AGTGAATCAG AACTCATTGA TGAGCTTTCA 
GAAGATTTTA ACCAGCCTAA AAGTAAAGGA GAACAGTCTA AGCCAACTGA AAAAACAGAA 
GAATCTCAGG CTGCCCCCCT AACTCCTGTG AGCGAGGCTG TGCCTCGGAC CTCCATGTGT 
AGCATACAGT CGGCACCGCC CAAGCCACCT ACCTTGGAAG GCATGGTGCC AGATGATGCT 
GTAGAAGCCT TGGCTGGTAG CCTGGGGAAG AAGGAAGCAG ATCCAGGAGA TGGAAAGCCT 
GTGGAAGATA AAGTCAAGGA GAAAGCCAAG GAAGAGGATC GTGAAAAACT TGGTGAAAAA 
GAAGAAACAA TTCCTCCTGA TTATAGATTA GAAGAGGTCA AGGATAAAGA TGGAAAACCA 
CTCCTGCCAA AAGTGCCCAA GGAATCGCTT TTACCCATGA GTGAAGACTT CCTTCTTGAT 
GCTTTGTCCA AGGACTTTGC TGGTTCCTCA AACACTTCAC CTCTTGAATT TGAAGAGGCT 
AACCTTCCTG CTGTCATCTC TGAAGTGGTT TCCCAAACCC CAGCTCCAAC CACGCACTCT 
GCTGGTCTAC CCCCTGATAC TGTGCAAAGT GACAACAAAG AACTGGACGA TGCTTTGGAC 
CAACTTTCTG ACAGTCTGGG ACAAAGGCAG CCTGATCCAG ATGAGAACAA GCCGATAGAG 
GATAAAGTCA AGGAAAGAGC TAAAGCTGAA CACAGAGACA AGCTGGGAGA AAGAGACGAC 
ACCATCCCCC CTGAATATAG ACACCTCTTG GATAACGATG AGGGTAAACC AGGAAAGCCA 
CCTGCCAAGA AAACCAAGGA CTCAAAGAAG CCTGAAGATG ACAAAGACCC CATCGATGCC 
CTGTCAGGCG ACTTCGACAG CTGTCCTGCA CCCACAGAAG CCTCAGAGAA CACAGCAGAG 
GACAAAGCCA AGAGAACTGC TCCCAGCTCC AAAACACCGA AGAATAGGGG TAAAGCAAAG 
GATTCTGCAA AGGCAAAGCA GGAAACTTCC AAGCCCAAAG CTGATGAAAA AAATACAAGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	CAST
Entrez Gene ID	479148
Full Name	calpastatin
General protein information	Preferred Names calpastatin Names calpastatin
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Homo sapiens (human)	CAST	NP_001035905.1
	CAST	XP_001091928.2
Mus musculus (house mouse)	Cast	NP_033947.1
Rattus norvegicus (Norway rat)	Cast	NP_445747.2
Gallus gallus (chicken)	CAST	NP_001131122.1
Pan troglodytes (chimpanzee)	CAST	XP_518168.4
Canis lupus familiaris (dog)	CAST	XP_005618134.1
Bos taurus (cattle)	CAST	NP_001025489.1
Xenopus tropicalis (tropical clawed frog)	cast	NP_001120309.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

CAST cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

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