GAK gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_022417285.1	XP_022272993.1	cyclin-G-associated kinase isoform X4
XM_022417290.1	XP_022272998.1	cyclin-G-associated kinase isoform X8
XM_005618685.2	XP_005618742.1	cyclin-G-associated kinase isoform X1
XM_005618686.2	XP_005618743.1	cyclin-G-associated kinase isoform X3
XM_005618687.2	XP_005618744.1	cyclin-G-associated kinase isoform X5
XM_536279.7	XP_536279.4	cyclin-G-associated kinase isoform X3
XM_005618684.2	XP_005618741.1	cyclin-G-associated kinase isoform X4
XM_005618689.2	XP_005618746.1	cyclin-G-associated kinase isoform X5
XM_005618684.3	XP_005618741.1	cyclin-G-associated kinase isoform X1
XM_022417287.1	XP_022272995.1	cyclin-G-associated kinase isoform X5
XM_005618687.3	XP_005618744.1	cyclin-G-associated kinase isoform X6
XM_022417295.1	XP_022273003.1	cyclin-G-associated kinase isoform X6
XM_014112678.1	XP_013968153.1	cyclin-G-associated kinase isoform X6
XM_005618688.2	XP_005618745.1	cyclin-G-associated kinase isoform X6
XM_005618689.3	XP_005618746.1	cyclin-G-associated kinase isoform X6
XM_022417291.1	XP_022272999.1	cyclin-G-associated kinase isoform X9
XM_005618688.3	XP_005618745.1	cyclin-G-associated kinase isoform X11
XM_022417293.1	XP_022273001.1	cyclin-G-associated kinase isoform X11
XM_536279.6	XP_536279.4	cyclin-G-associated kinase isoform X2
XM_005618685.3	XP_005618742.1	cyclin-G-associated kinase isoform X2
XM_022417288.1	XP_022272996.1	cyclin-G-associated kinase isoform X6
XM_022417289.1	XP_022272997.1	cyclin-G-associated kinase isoform X7
XM_022417292.1	XP_022273000.1	cyclin-G-associated kinase isoform X10

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe04649	XM_005618684.3 Latest version!	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCe04650	XM_005618685.3 Latest version!	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCe04651	XM_536279.7 Latest version!	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCe04652	XM_005618686.2 Latest version!	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCe04652	XM_022417287.1 Latest version!	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCe04653	XM_005618687.3 Latest version!	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCe04653	XM_022417288.1 Latest version!	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCe04653	XM_005618689.3 Latest version!	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCe04653	XM_022417295.1 Latest version!	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCe04654	XM_022417293.1 Latest version!	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OCe04654	XM_014112678.1 Latest version!	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OCe04654	XM_005618688.3 Latest version!	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OCe287420	XM_022417285.1 Latest version!	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCe287421	XM_022417289.1 Latest version!	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCe287422	XM_022417290.1 Latest version!	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCe287423	XM_022417291.1 Latest version!	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OCe287424	XM_022417292.1 Latest version!	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
View Old Accession Versions >>
OCe04649	XM_005618684.2	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCe04653	XM_005618687.2	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCe04653	XM_005618689.2	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCe04654	XM_005618688.2	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OCe04651	XM_536279.6	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCe04650	XM_005618685.2	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
Hide Old Accession Versions >>

GAK ( XM_005618684.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005618741.1 Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe04649
Clone ID Related Accession (Same CDS sequence)	XM_005618684.2 , XM_005618684.3
Accession Version	XM_005618684.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	cyclin-G-associated kinase isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005618684.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGTTT CCCCCAAGGC CGGGGTGAAG AGCGCAGGTT TGGGTGCGAT CCGGTACGCA 
GAGGCGGGGG CCGCTGACGC CCGGATGCTG TCGGCCGCCC CGGGGCGCTG CAGCCGTCGC 
CGCCGTCGGG GCCTGTGCTC CGACAACACC GAACACTTGT GCCGGGTTTT TAATACGAGA 
TTCACGGTGG ACAGAGGGTT TGCATTTGTG TATGAAGCTC AAGATCTGGC AAGTGGCAGA 
GAATATGCCT TAAAGAGATT ATTGTCTAAC GAAGAGGAAA AGAACAGAGC CATCATTCAG 
GAAGTTTGTT TCATGAAAAA ACTTTCTGGC CACCCCAATA TTGTCCAGTT TTGTTCTGCA 
GCGTCCATAG GGAAAGAGGA GTCGGACACT GGGCAGGCTG AGTTCCTGCT GCTCACCGAG 
CTCTGCAAAG GGCAGCTGGT GGACTTTTTA AAGAAAATTG AATCTAGAGG TCCGCTCTCG 
TGCGATACTG TTCTGAAGAT TTTCTATCAG ACGTGCAGAG CGGTGCAGCA CATGCACAGA 
CAAAAGCCGC CCATCATCCA TAGAGACCTC AAGGTGGAAA ACTTATTGCT CAGTAACCAA 
GGGACCATCA AGCTGTGTGA CTTTGGCAGT GCCACGACCA TATCACATTA CCCCGACTAC 
AGCTGGAGCG CCCAGAGGCG GGCCCTGGTG GAGGAAGAGA TCACCAGGAA CACCACACCC 
ATGTACAGGA CACCGGAAAT CGTGGACTTG TACTCCAACT TCCCCATCGG CGAGAAGCAG 
GACATCTGGG CGCTGGGCTG CATCTTGTAT CTGCTGTGCT TCCGGCAGCA TCCTTTTGAA 
GACGGAGCAA AGCTTCGGAT AGTCAACGGG AAGTTCTCGA TCCCCCCCAA TGACACCCGG 
TACTCTGTGT TCCACGACCT CATCCGCGCC ACGCTGAAGG TCAACCCCGA GGAGCGGCTG 
TCCATCACAG AACTGGTGAA CCAGCTCCAG GAGATCGCGG CCGCCAGGAA CGTGAACCCC 
AAGTCTCCCA TCACTGAGCT CCTAGAGCAG AACGGAGGCT ATGGGAACGC TGTCCCTTCC 
CGAGGGCCAC CCCCTCCTGG GGGCTCCAGC AGCGGCTACA GCGGAGGCCT GGCGCTGGCC 
GAGTACGACC AGCCCTACGG CGGGCTCCTG GACATCCTGC GGGGGGGCAC GGAGCGCCTC 
TTCACAAACC TCAAGGACAC CTCCTCCAAG GTCATCCAGT CTGTTGCCAA TTACGCAAAA 
GGAGATTTGG ACATCTCTTA CATCACATCT AGAATTGCTG TGATGTCGTT CCCAGCAGAA 
GGCGTGGAGT CGGCCATCAA GAACAACATC GAAGACGTGC GGTTGTTTCT GGACTCCAAG 
CACCCGGGAC ATTACGCCGT CTACAACCTG TCCCCAAGAA CATACCGGCC CTCCAAATTT 
CACAACCGGG TCTCCGAGTG TGGCTGGGCG GCCAGACGGG CCCCGAACCT TCGCAGCCTG 
TACAGTGTGT GTCGGAACAT GCACTCGTGG CTCCGGCAGG ACCACAAGAA CGTCTGTGTC 
GTGCACTGCA TGGACGGGAG AGCTGCGTCT GCGGTGGCCG TGTGCTCCTT CCTGTGCTTC 
TGCCGCCTGT TCAGCACCGC GGAGGCTGCC GTGTACATGT TCAGCATGAA GCGCTGCCCG 
CCAGGCATTT GGCCATCCCA CAAGAGGTAC ATCGAGTACA TGTGTGACAT GGTGGCCGAG 
GAGCCCATCA CACCCCATAG CAAGCCCATC CTGGTGAAAG CCGTGGTCAT GACACCCGTG 
CCCCTGTTCA GCAAGCAGAG AAACGGCTGC AGGCCCTTCT GCGAGGTCTA CGTGGGGGAT 
GAGCGCGTGA CTACCACGTC TCAGGAGTAC GACAAGATGA GGGATTTCAG AATCGAGGAT 
GGCAAAGCAG TCATTCCCCT GGGCATAACA GTGCAAGGGG ACGTGCTCAT TGTCATCTAC 
CACGCCAGGT CCACCCTGGG AGGAAGACTG CAGGCCAAGG TGGCATCCAT GAAGATGTTC 
CAGGTCCAGT TCCACACGGG GTTTGTGCCT CGGAATGCGA CTACTGTGAA GTTTGCAAAG 
TATGACCTTG ATGCGTGTGA CATTCAGGAG AAATACCCAG ATTTATTCCA AGTGAACGTG 
GAGGTGGAGG TGGAGCCCAG AGACAGGCCC AGCCGGGAGG CCCCACCCTG GGAGAGCACC 
AGCATGAGGG GGCTCAACCC CAAAATCCTG TTTTCCAGCC GAGAGGAGCA GCAAGACATT 
CTGTCTAAAT TTGGGAAGCC GGAGCTCCCC AGGCAGCCCG GCTCCACCGC TCAGTACGAC 
GCCGAGGCAG GGTCCCTGGA CGCAGAGCCC ACAGAGTCTG ATTCACCACA GAGCAGCGGC 
ACTGACACCA GCCACTTCCT CCACACGCTG GATTGGCACG AGGAGAAAGA TACAGAGACG 
AGTCCAGAAA GTCACTTTGC CAAGGAGAAC CTATCAACCC TGGCCGAGGA TGCAGGTGGG 
AGTGAGCCTT CGGACGAGGA AGCAGCCACA CCATCCACCG AGAGCAGGGA TGCTGCGGAT 
GAGGACACAC CAGGGCCAGC GGCGAGGCTG CCAGAGCGAG ATTTGATTTT TGATGGGGCC 
ACGCCGGCCA CACCGCAGGA GCCCACACGG CAGGAGGACA GTGTCGACCT CCTAGGGCTG 
CATGCCGAGG CTGGGCTGGC CCCCCCTGTG CAGGCCAGTG GGGCAGCCCC CAGCACCGCC 
GACCTGCTCA GCCGCCTCCT GGGGGGCCCT GACGCTGCTC CGGAGGTGCC CCCGGGTGAC 
CTGCTGGGTG GAGAGGCTCC CCTCCTCTTC GCAAGCCCAG CCCCCTCCTC GAGCGCACAG 
AGCCCGCCCC GTGAGGGGCC AGCTGCCGAC CCCTTTGACC CTCTCCTGCT GCCATCTGGG 
CCCAACACCC AGCCGTGCTC CCAGCCTGAC CTGTTCGGTG AATTTCTTCA TTCGGAATCT 
CCTGCCGCCG TGCCTGCGTC CTTCCCGTCC ACCCACAGCG CCCCACCGCC CGCCTGCAGC 
ACCGACTTCC TGCACCTGGG AGATCTACCA GCAGAGCCTG GCAAGATGAC GGCCTCCACC 
AGCCACCCGG ATCTGCTGGG GGGGTGGGAG GCCTGGGCCG AGGCGCCGGC CCCCGCAGCT 
GCTGCAGAAG GTCCCTTCTT CTCTGCAGGA GGCCAGCCTG CCGCCCCCAG TGCCCCCGGG 
AGCTGGACCA AGTCTCAGAG CCTGGACCCG TTTGCTGACC TCGGTGACCT CAGTTCTGGC 
CTCCAAGGCT CACCGGCTGG ATTTGCTCCA GGGGCTTTTG GCGCCAAGAC GGCCCCCCCT 
CCCACGGGCG GCAGCTCCTG GCAGCAGACG GGTCGGCCCC CGGCCCAGGG CACCCCGTGG 
CCCCCCCAGG CCAAGCCGCC CCCCAAGGCC TGTCCGCAGC CAAGGCCTAA CTATGCTGCC 
AACGTGAGCG TGATTGGCGG CCGAGAGGAG AGAGGGGTCC GGGCCCCCAG CTTCGGCCAG 
AAGCCGAGAG TGTCCGAGAG CGACTTTGAG GACCTGCTGT CCCACCAGGG CTTCTCGTCC 
AAGGCTGACC GGAAGGGGCC AAGGACCATG GCTGAGATGC GGCGGCAGGA GCAGGCGAGG 
GACGCAGACC CGCTGAAGCT GAAGCTCCTG GAGTGGACAG AGGGCAAGGA GAGGAACATC 
CGCGCGCTGC TGTCGACCCT GCACACCGTG CTGTGGGACG GCGAGAGCCG CTGGGCCCCC 
GTGGGCATGG CCGACCTGGT GACCCCCGGG CAGGTGAAGA AGCAGTACCG CCGCGCCGTG 
CTGGTGGTGC ACCCGGACAA GGCCGCAGGG CAGCCCTACG AGCAGTATGC CAAGATGATC 
TTCATGGAGC TGAACGACGC GTGGGCCGAG TTTGAGAGCC AGGGCTCCCG GCCGCTCTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005618741.1
CDS	87..4049
Translation	MRVSPKAGVKSAGLGAIRYAEAGAADARMLSAAPGRCSRRRRRGLCSDNTEHLCRVFNTRFTVDRGFAFVYEAQDLASGREYALKRLLSNEEEKNRAIIQEVCFMKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLTELCKGQLVDFLKKIESRGPLSCDTVLKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKFSIPPNDTRYSVFHDLIRATLKVNPEERLSITELVNQLQEIAAARNVNPKSPITELLEQNGGYGNAVPSRGPPPPGGSSSGYSGGLALAEYDQPYGGLLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRLFLDSKHPGHYAVYNLSPRTYRPSKFHNRVSECGWAARRAPNLRSLYSVCRNMHSWLRQDHKNVCVVHCMDGRAASAVAVCSFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYMCDMVAEEPITPHSKPILVKAVVMTPVPLFSKQRNGCRPFCEVYVGDERVTTTSQEYDKMRDFRIEDGKAVIPLGITVQGDVLIVIYHARSTLGGRLQAKVASMKMFQVQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNVEVEVEPRDRPSREAPPWESTSMRGLNPKILFSSREEQQDILSKFGKPELPRQPGSTAQYDAEAGSLDAEPTESDSPQSSGTDTSHFLHTLDWHEEKDTETSPESHFAKENLSTLAEDAGGSEPSDEEAATPSTESRDAADEDTPGPAARLPERDLIFDGATPATPQEPTRQEDSVDLLGLHAEAGLAPPVQASGAAPSTADLLSRLLGGPDAAPEVPPGDLLGGEAPLLFASPAPSSSAQSPPREGPAADPFDPLLLPSGPNTQPCSQPDLFGEFLHSESPAAVPASFPSTHSAPPPACSTDFLHLGDLPAEPGKMTASTSHPDLLGGWEAWAEAPAPAAAAEGPFFSAGGQPAAPSAPGSWTKSQSLDPFADLGDLSSGLQGSPAGFAPGAFGAKTAPPPTGGSSWQQTGRPPAQGTPWPPQAKPPPKACPQPRPNYAANVSVIGGREERGVRAPSFGQKPRVSESDFEDLLSHQGFSSKADRKGPRTMAEMRRQEQARDADPLKLKLLEWTEGKERNIRALLSTLHTVLWDGESRWAPVGMADLVTPGQVKKQYRRAVLVVHPDKAAGQPYEQYAKMIFMELNDAWAEFESQGSRPLF

Target ORF information:

RefSeq Version	XM_005618684.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005618684.2

ORF Insert Sequence:

ATGCGTGTTT CCCCCAAGGC CGGGGTGAAG AGCGCAGGTT TGGGTGCGAT CCGGTACGCA 
GAGGCGGGGG CCGCTGACGC CCGGATGCTG TCGGCCGCCC CGGGGCGCTG CAGCCGTCGC 
CGCCGTCGGG GCCTGTGCTC CGACAACACC GAACACTTGT GCCGGGTTTT TAATACGAGA 
TTCACGGTGG ACAGAGGGTT TGCATTTGTG TATGAAGCTC AAGATCTGGC AAGTGGCAGA 
GAATATGCCT TAAAGAGATT ATTGTCTAAC GAAGAGGAAA AGAACAGAGC CATCATTCAG 
GAAGTTTGTT TCATGAAAAA ACTTTCTGGC CACCCCAATA TTGTCCAGTT TTGTTCTGCA 
GCGTCCATAG GGAAAGAGGA GTCGGACACT GGGCAGGCTG AGTTCCTGCT GCTCACCGAG 
CTCTGCAAAG GGCAGCTGGT GGACTTTTTA AAGAAAATTG AATCTAGAGG TCCGCTCTCG 
TGCGATACTG TTCTGAAGAT TTTCTATCAG ACGTGCAGAG CGGTGCAGCA CATGCACAGA 
CAAAAGCCGC CCATCATCCA TAGAGACCTC AAGGTGGAAA ACTTATTGCT CAGTAACCAA 
GGGACCATCA AGCTGTGTGA CTTTGGCAGT GCCACGACCA TATCACATTA CCCCGACTAC 
AGCTGGAGCG CCCAGAGGCG GGCCCTGGTG GAGGAAGAGA TCACCAGGAA CACCACACCC 
ATGTACAGGA CACCGGAAAT CGTGGACTTG TACTCCAACT TCCCCATCGG CGAGAAGCAG 
GACATCTGGG CGCTGGGCTG CATCTTGTAT CTGCTGTGCT TCCGGCAGCA TCCTTTTGAA 
GACGGAGCAA AGCTTCGGAT AGTCAACGGG AAGTTCTCGA TCCCCCCCAA TGACACCCGG 
TACTCTGTGT TCCACGACCT CATCCGCGCC ACGCTGAAGG TCAACCCCGA GGAGCGGCTG 
TCCATCACAG AACTGGTGAA CCAGCTCCAG GAGATCGCGG CCGCCAGGAA CGTGAACCCC 
AAGTCTCCCA TCACTGAGCT CCTAGAGCAG AACGGAGGCT ATGGGAACGC TGTCCCTTCC 
CGAGGGCCAC CCCCTCCTGG GGGCTCCAGC AGCGGCTACA GCGGAGGCCT GGCGCTGGCC 
GAGTACGACC AGCCCTACGG CGGGCTCCTG GACATCCTGC GGGGGGGCAC GGAGCGCCTC 
TTCACAAACC TCAAGGACAC CTCCTCCAAG GTCATCCAGT CTGTTGCCAA TTACGCAAAA 
GGAGATTTGG ACATCTCTTA CATCACATCT AGAATTGCTG TGATGTCGTT CCCAGCAGAA 
GGCGTGGAGT CGGCCATCAA GAACAACATC GAAGACGTGC GGTTGTTTCT GGACTCCAAG 
CACCCGGGAC ATTACGCCGT CTACAACCTG TCCCCAAGAA CATACCGGCC CTCCAAATTT 
CACAACCGGG TCTCCGAGTG TGGCTGGGCG GCCAGACGGG CCCCGAACCT TCGCAGCCTG 
TACAGTGTGT GTCGGAACAT GCACTCGTGG CTCCGGCAGG ACCACAAGAA CGTCTGTGTC 
GTGCACTGCA TGGACGGGAG AGCTGCGTCT GCGGTGGCCG TGTGCTCCTT CCTGTGCTTC 
TGCCGCCTGT TCAGCACCGC GGAGGCTGCC GTGTACATGT TCAGCATGAA GCGCTGCCCG 
CCAGGCATTT GGCCATCCCA CAAGAGGTAC ATCGAGTACA TGTGTGACAT GGTGGCCGAG 
GAGCCCATCA CACCCCATAG CAAGCCCATC CTGGTGAAAG CCGTGGTCAT GACACCCGTG 
CCCCTGTTCA GCAAGCAGAG AAACGGCTGC AGGCCCTTCT GCGAGGTCTA CGTGGGGGAT 
GAGCGCGTGA CTACCACGTC TCAGGAGTAC GACAAGATGA GGGATTTCAG AATCGAGGAT 
GGCAAAGCAG TCATTCCCCT GGGCATAACA GTGCAAGGGG ACGTGCTCAT TGTCATCTAC 
CACGCCAGGT CCACCCTGGG AGGAAGACTG CAGGCCAAGG TGGCATCCAT GAAGATGTTC 
CAGGTCCAGT TCCACACGGG GTTTGTGCCT CGGAATGCGA CTACTGTGAA GTTTGCAAAG 
TATGACCTTG ATGCGTGTGA CATTCAGGAG AAATACCCAG ATTTATTCCA AGTGAACGTG 
GAGGTGGAGG TGGAGCCCAG AGACAGGCCC AGCCGGGAGG CCCCACCCTG GGAGAGCACC 
AGCATGAGGG GGCTCAACCC CAAAATCCTG TTTTCCAGCC GAGAGGAGCA GCAAGACATT 
CTGTCTAAAT TTGGGAAGCC GGAGCTCCCC AGGCAGCCCG GCTCCACCGC TCAGTACGAC 
GCCGAGGCAG GGTCCCTGGA CGCAGAGCCC ACAGAGTCTG ATTCACCACA GAGCAGCGGC 
ACTGACACCA GCCACTTCCT CCACACGCTG GATTGGCACG AGGAGAAAGA TACAGAGACG 
AGTCCAGAAA GTCACTTTGC CAAGGAGAAC CTATCAACCC TGGCCGAGGA TGCAGGTGGG 
AGTGAGCCTT CGGACGAGGA AGCAGCCACA CCATCCACCG AGAGCAGGGA TGCTGCGGAT 
GAGGACACAC CAGGGCCAGC GGCGAGGCTG CCAGAGCGAG ATTTGATTTT TGATGGGGCC 
ACGCCGGCCA CACCGCAGGA GCCCACACGG CAGGAGGACA GTGTCGACCT CCTAGGGCTG 
CATGCCGAGG CTGGGCTGGC CCCCCCTGTG CAGGCCAGTG GGGCAGCCCC CAGCACCGCC 
GACCTGCTCA GCCGCCTCCT GGGGGGCCCT GACGCTGCTC CGGAGGTGCC CCCGGGTGAC 
CTGCTGGGTG GAGAGGCTCC CCTCCTCTTC GCAAGCCCAG CCCCCTCCTC GAGCGCACAG 
AGCCCGCCCC GTGAGGGGCC AGCTGCCGAC CCCTTTGACC CTCTCCTGCT GCCATCTGGG 
CCCAACACCC AGCCGTGCTC CCAGCCTGAC CTGTTCGGTG AATTTCTTCA TTCGGAATCT 
CCTGCCGCCG TGCCTGCGTC CTTCCCGTCC ACCCACAGCG CCCCACCGCC CGCCTGCAGC 
ACCGACTTCC TGCACCTGGG AGATCTACCA GCAGAGCCTG GCAAGATGAC GGCCTCCACC 
AGCCACCCGG ATCTGCTGGG GGGGTGGGAG GCCTGGGCCG AGGCGCCGGC CCCCGCAGCT 
GCTGCAGAAG GTCCCTTCTT CTCTGCAGGA GGCCAGCCTG CCGCCCCCAG TGCCCCCGGG 
AGCTGGACCA AGTCTCAGAG CCTGGACCCG TTTGCTGACC TCGGTGACCT CAGTTCTGGC 
CTCCAAGGCT CACCGGCTGG ATTTGCTCCA GGGGCTTTTG GCGCCAAGAC GGCCCCCCCT 
CCCACGGGCG GCAGCTCCTG GCAGCAGACG GGTCGGCCCC CGGCCCAGGG CACCCCGTGG 
CCCCCCCAGG CCAAGCCGCC CCCCAAGGCC TGTCCGCAGC CAAGGCCTAA CTATGCTGCC 
AACGTGAGCG TGATTGGCGG CCGAGAGGAG AGAGGGGTCC GGGCCCCCAG CTTCGGCCAG 
AAGCCGAGAG TGTCCGAGAG CGACTTTGAG GACCTGCTGT CCCACCAGGG CTTCTCGTCC 
AAGGCTGACC GGAAGGGGCC AAGGACCATG GCTGAGATGC GGCGGCAGGA GCAGGCGAGG 
GACGCAGACC CGCTGAAGCT GAAGCTCCTG GAGTGGACAG AGGGCAAGGA GAGGAACATC 
CGCGCGCTGC TGTCGACCCT GCACACCGTG CTGTGGGACG GCGAGAGCCG CTGGGCCCCC 
GTGGGCATGG CCGACCTGGT GACCCCCGGG CAGGTGAAGA AGCAGTACCG CCGCGCCGTG 
CTGGTGGTGC ACCCGGACAA GGCCGCAGGG CAGCCCTACG AGCAGTATGC CAAGATGATC 
TTCATGGAGC TGAACGACGC GTGGGCCGAG TTTGAGAGCC AGGGCTCCCG GCCGCTCTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAK ( XM_005618684.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005618741.1 Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe04649
Clone ID Related Accession (Same CDS sequence)	XM_005618684.2 , XM_005618684.3
Accession Version	XM_005618684.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	cyclin-G-associated kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005618684.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGTTT CCCCCAAGGC CGGGGTGAAG AGCGCAGGTT TGGGTGCGAT CCGGTACGCA 
GAGGCGGGGG CCGCTGACGC CCGGATGCTG TCGGCCGCCC CGGGGCGCTG CAGCCGTCGC 
CGCCGTCGGG GCCTGTGCTC CGACAACACC GAACACTTGT GCCGGGTTTT TAATACGAGA 
TTCACGGTGG ACAGAGGGTT TGCATTTGTG TATGAAGCTC AAGATCTGGC AAGTGGCAGA 
GAATATGCCT TAAAGAGATT ATTGTCTAAC GAAGAGGAAA AGAACAGAGC CATCATTCAG 
GAAGTTTGTT TCATGAAAAA ACTTTCTGGC CACCCCAATA TTGTCCAGTT TTGTTCTGCA 
GCGTCCATAG GGAAAGAGGA GTCGGACACT GGGCAGGCTG AGTTCCTGCT GCTCACCGAG 
CTCTGCAAAG GGCAGCTGGT GGACTTTTTA AAGAAAATTG AATCTAGAGG TCCGCTCTCG 
TGCGATACTG TTCTGAAGAT TTTCTATCAG ACGTGCAGAG CGGTGCAGCA CATGCACAGA 
CAAAAGCCGC CCATCATCCA TAGAGACCTC AAGGTGGAAA ACTTATTGCT CAGTAACCAA 
GGGACCATCA AGCTGTGTGA CTTTGGCAGT GCCACGACCA TATCACATTA CCCCGACTAC 
AGCTGGAGCG CCCAGAGGCG GGCCCTGGTG GAGGAAGAGA TCACCAGGAA CACCACACCC 
ATGTACAGGA CACCGGAAAT CGTGGACTTG TACTCCAACT TCCCCATCGG CGAGAAGCAG 
GACATCTGGG CGCTGGGCTG CATCTTGTAT CTGCTGTGCT TCCGGCAGCA TCCTTTTGAA 
GACGGAGCAA AGCTTCGGAT AGTCAACGGG AAGTTCTCGA TCCCCCCCAA TGACACCCGG 
TACTCTGTGT TCCACGACCT CATCCGCGCC ACGCTGAAGG TCAACCCCGA GGAGCGGCTG 
TCCATCACAG AACTGGTGAA CCAGCTCCAG GAGATCGCGG CCGCCAGGAA CGTGAACCCC 
AAGTCTCCCA TCACTGAGCT CCTAGAGCAG AACGGAGGCT ATGGGAACGC TGTCCCTTCC 
CGAGGGCCAC CCCCTCCTGG GGGCTCCAGC AGCGGCTACA GCGGAGGCCT GGCGCTGGCC 
GAGTACGACC AGCCCTACGG CGGGCTCCTG GACATCCTGC GGGGGGGCAC GGAGCGCCTC 
TTCACAAACC TCAAGGACAC CTCCTCCAAG GTCATCCAGT CTGTTGCCAA TTACGCAAAA 
GGAGATTTGG ACATCTCTTA CATCACATCT AGAATTGCTG TGATGTCGTT CCCAGCAGAA 
GGCGTGGAGT CGGCCATCAA GAACAACATC GAAGACGTGC GGTTGTTTCT GGACTCCAAG 
CACCCGGGAC ATTACGCCGT CTACAACCTG TCCCCAAGAA CATACCGGCC CTCCAAATTT 
CACAACCGGG TCTCCGAGTG TGGCTGGGCG GCCAGACGGG CCCCGAACCT TCGCAGCCTG 
TACAGTGTGT GTCGGAACAT GCACTCGTGG CTCCGGCAGG ACCACAAGAA CGTCTGTGTC 
GTGCACTGCA TGGACGGGAG AGCTGCGTCT GCGGTGGCCG TGTGCTCCTT CCTGTGCTTC 
TGCCGCCTGT TCAGCACCGC GGAGGCTGCC GTGTACATGT TCAGCATGAA GCGCTGCCCG 
CCAGGCATTT GGCCATCCCA CAAGAGGTAC ATCGAGTACA TGTGTGACAT GGTGGCCGAG 
GAGCCCATCA CACCCCATAG CAAGCCCATC CTGGTGAAAG CCGTGGTCAT GACACCCGTG 
CCCCTGTTCA GCAAGCAGAG AAACGGCTGC AGGCCCTTCT GCGAGGTCTA CGTGGGGGAT 
GAGCGCGTGA CTACCACGTC TCAGGAGTAC GACAAGATGA GGGATTTCAG AATCGAGGAT 
GGCAAAGCAG TCATTCCCCT GGGCATAACA GTGCAAGGGG ACGTGCTCAT TGTCATCTAC 
CACGCCAGGT CCACCCTGGG AGGAAGACTG CAGGCCAAGG TGGCATCCAT GAAGATGTTC 
CAGGTCCAGT TCCACACGGG GTTTGTGCCT CGGAATGCGA CTACTGTGAA GTTTGCAAAG 
TATGACCTTG ATGCGTGTGA CATTCAGGAG AAATACCCAG ATTTATTCCA AGTGAACGTG 
GAGGTGGAGG TGGAGCCCAG AGACAGGCCC AGCCGGGAGG CCCCACCCTG GGAGAGCACC 
AGCATGAGGG GGCTCAACCC CAAAATCCTG TTTTCCAGCC GAGAGGAGCA GCAAGACATT 
CTGTCTAAAT TTGGGAAGCC GGAGCTCCCC AGGCAGCCCG GCTCCACCGC TCAGTACGAC 
GCCGAGGCAG GGTCCCTGGA CGCAGAGCCC ACAGAGTCTG ATTCACCACA GAGCAGCGGC 
ACTGACACCA GCCACTTCCT CCACACGCTG GATTGGCACG AGGAGAAAGA TACAGAGACG 
AGTCCAGAAA GTCACTTTGC CAAGGAGAAC CTATCAACCC TGGCCGAGGA TGCAGGTGGG 
AGTGAGCCTT CGGACGAGGA AGCAGCCACA CCATCCACCG AGAGCAGGGA TGCTGCGGAT 
GAGGACACAC CAGGGCCAGC GGCGAGGCTG CCAGAGCGAG ATTTGATTTT TGATGGGGCC 
ACGCCGGCCA CACCGCAGGA GCCCACACGG CAGGAGGACA GTGTCGACCT CCTAGGGCTG 
CATGCCGAGG CTGGGCTGGC CCCCCCTGTG CAGGCCAGTG GGGCAGCCCC CAGCACCGCC 
GACCTGCTCA GCCGCCTCCT GGGGGGCCCT GACGCTGCTC CGGAGGTGCC CCCGGGTGAC 
CTGCTGGGTG GAGAGGCTCC CCTCCTCTTC GCAAGCCCAG CCCCCTCCTC GAGCGCACAG 
AGCCCGCCCC GTGAGGGGCC AGCTGCCGAC CCCTTTGACC CTCTCCTGCT GCCATCTGGG 
CCCAACACCC AGCCGTGCTC CCAGCCTGAC CTGTTCGGTG AATTTCTTCA TTCGGAATCT 
CCTGCCGCCG TGCCTGCGTC CTTCCCGTCC ACCCACAGCG CCCCACCGCC CGCCTGCAGC 
ACCGACTTCC TGCACCTGGG AGATCTACCA GCAGAGCCTG GCAAGATGAC GGCCTCCACC 
AGCCACCCGG ATCTGCTGGG GGGGTGGGAG GCCTGGGCCG AGGCGCCGGC CCCCGCAGCT 
GCTGCAGAAG GTCCCTTCTT CTCTGCAGGA GGCCAGCCTG CCGCCCCCAG TGCCCCCGGG 
AGCTGGACCA AGTCTCAGAG CCTGGACCCG TTTGCTGACC TCGGTGACCT CAGTTCTGGC 
CTCCAAGGCT CACCGGCTGG ATTTGCTCCA GGGGCTTTTG GCGCCAAGAC GGCCCCCCCT 
CCCACGGGCG GCAGCTCCTG GCAGCAGACG GGTCGGCCCC CGGCCCAGGG CACCCCGTGG 
CCCCCCCAGG CCAAGCCGCC CCCCAAGGCC TGTCCGCAGC CAAGGCCTAA CTATGCTGCC 
AACGTGAGCG TGATTGGCGG CCGAGAGGAG AGAGGGGTCC GGGCCCCCAG CTTCGGCCAG 
AAGCCGAGAG TGTCCGAGAG CGACTTTGAG GACCTGCTGT CCCACCAGGG CTTCTCGTCC 
AAGGCTGACC GGAAGGGGCC AAGGACCATG GCTGAGATGC GGCGGCAGGA GCAGGCGAGG 
GACGCAGACC CGCTGAAGCT GAAGCTCCTG GAGTGGACAG AGGGCAAGGA GAGGAACATC 
CGCGCGCTGC TGTCGACCCT GCACACCGTG CTGTGGGACG GCGAGAGCCG CTGGGCCCCC 
GTGGGCATGG CCGACCTGGT GACCCCCGGG CAGGTGAAGA AGCAGTACCG CCGCGCCGTG 
CTGGTGGTGC ACCCGGACAA GGCCGCAGGG CAGCCCTACG AGCAGTATGC CAAGATGATC 
TTCATGGAGC TGAACGACGC GTGGGCCGAG TTTGAGAGCC AGGGCTCCCG GCCGCTCTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005618741.1
CDS	16..3978
Translation	MRVSPKAGVKSAGLGAIRYAEAGAADARMLSAAPGRCSRRRRRGLCSDNTEHLCRVFNTRFTVDRGFAFVYEAQDLASGREYALKRLLSNEEEKNRAIIQEVCFMKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLTELCKGQLVDFLKKIESRGPLSCDTVLKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKFSIPPNDTRYSVFHDLIRATLKVNPEERLSITELVNQLQEIAAARNVNPKSPITELLEQNGGYGNAVPSRGPPPPGGSSSGYSGGLALAEYDQPYGGLLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRLFLDSKHPGHYAVYNLSPRTYRPSKFHNRVSECGWAARRAPNLRSLYSVCRNMHSWLRQDHKNVCVVHCMDGRAASAVAVCSFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYMCDMVAEEPITPHSKPILVKAVVMTPVPLFSKQRNGCRPFCEVYVGDERVTTTSQEYDKMRDFRIEDGKAVIPLGITVQGDVLIVIYHARSTLGGRLQAKVASMKMFQVQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNVEVEVEPRDRPSREAPPWESTSMRGLNPKILFSSREEQQDILSKFGKPELPRQPGSTAQYDAEAGSLDAEPTESDSPQSSGTDTSHFLHTLDWHEEKDTETSPESHFAKENLSTLAEDAGGSEPSDEEAATPSTESRDAADEDTPGPAARLPERDLIFDGATPATPQEPTRQEDSVDLLGLHAEAGLAPPVQASGAAPSTADLLSRLLGGPDAAPEVPPGDLLGGEAPLLFASPAPSSSAQSPPREGPAADPFDPLLLPSGPNTQPCSQPDLFGEFLHSESPAAVPASFPSTHSAPPPACSTDFLHLGDLPAEPGKMTASTSHPDLLGGWEAWAEAPAPAAAAEGPFFSAGGQPAAPSAPGSWTKSQSLDPFADLGDLSSGLQGSPAGFAPGAFGAKTAPPPTGGSSWQQTGRPPAQGTPWPPQAKPPPKACPQPRPNYAANVSVIGGREERGVRAPSFGQKPRVSESDFEDLLSHQGFSSKADRKGPRTMAEMRRQEQARDADPLKLKLLEWTEGKERNIRALLSTLHTVLWDGESRWAPVGMADLVTPGQVKKQYRRAVLVVHPDKAAGQPYEQYAKMIFMELNDAWAEFESQGSRPLF

Target ORF information:

RefSeq Version	XM_005618684.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005618684.3

ORF Insert Sequence:

ATGCGTGTTT CCCCCAAGGC CGGGGTGAAG AGCGCAGGTT TGGGTGCGAT CCGGTACGCA 
GAGGCGGGGG CCGCTGACGC CCGGATGCTG TCGGCCGCCC CGGGGCGCTG CAGCCGTCGC 
CGCCGTCGGG GCCTGTGCTC CGACAACACC GAACACTTGT GCCGGGTTTT TAATACGAGA 
TTCACGGTGG ACAGAGGGTT TGCATTTGTG TATGAAGCTC AAGATCTGGC AAGTGGCAGA 
GAATATGCCT TAAAGAGATT ATTGTCTAAC GAAGAGGAAA AGAACAGAGC CATCATTCAG 
GAAGTTTGTT TCATGAAAAA ACTTTCTGGC CACCCCAATA TTGTCCAGTT TTGTTCTGCA 
GCGTCCATAG GGAAAGAGGA GTCGGACACT GGGCAGGCTG AGTTCCTGCT GCTCACCGAG 
CTCTGCAAAG GGCAGCTGGT GGACTTTTTA AAGAAAATTG AATCTAGAGG TCCGCTCTCG 
TGCGATACTG TTCTGAAGAT TTTCTATCAG ACGTGCAGAG CGGTGCAGCA CATGCACAGA 
CAAAAGCCGC CCATCATCCA TAGAGACCTC AAGGTGGAAA ACTTATTGCT CAGTAACCAA 
GGGACCATCA AGCTGTGTGA CTTTGGCAGT GCCACGACCA TATCACATTA CCCCGACTAC 
AGCTGGAGCG CCCAGAGGCG GGCCCTGGTG GAGGAAGAGA TCACCAGGAA CACCACACCC 
ATGTACAGGA CACCGGAAAT CGTGGACTTG TACTCCAACT TCCCCATCGG CGAGAAGCAG 
GACATCTGGG CGCTGGGCTG CATCTTGTAT CTGCTGTGCT TCCGGCAGCA TCCTTTTGAA 
GACGGAGCAA AGCTTCGGAT AGTCAACGGG AAGTTCTCGA TCCCCCCCAA TGACACCCGG 
TACTCTGTGT TCCACGACCT CATCCGCGCC ACGCTGAAGG TCAACCCCGA GGAGCGGCTG 
TCCATCACAG AACTGGTGAA CCAGCTCCAG GAGATCGCGG CCGCCAGGAA CGTGAACCCC 
AAGTCTCCCA TCACTGAGCT CCTAGAGCAG AACGGAGGCT ATGGGAACGC TGTCCCTTCC 
CGAGGGCCAC CCCCTCCTGG GGGCTCCAGC AGCGGCTACA GCGGAGGCCT GGCGCTGGCC 
GAGTACGACC AGCCCTACGG CGGGCTCCTG GACATCCTGC GGGGGGGCAC GGAGCGCCTC 
TTCACAAACC TCAAGGACAC CTCCTCCAAG GTCATCCAGT CTGTTGCCAA TTACGCAAAA 
GGAGATTTGG ACATCTCTTA CATCACATCT AGAATTGCTG TGATGTCGTT CCCAGCAGAA 
GGCGTGGAGT CGGCCATCAA GAACAACATC GAAGACGTGC GGTTGTTTCT GGACTCCAAG 
CACCCGGGAC ATTACGCCGT CTACAACCTG TCCCCAAGAA CATACCGGCC CTCCAAATTT 
CACAACCGGG TCTCCGAGTG TGGCTGGGCG GCCAGACGGG CCCCGAACCT TCGCAGCCTG 
TACAGTGTGT GTCGGAACAT GCACTCGTGG CTCCGGCAGG ACCACAAGAA CGTCTGTGTC 
GTGCACTGCA TGGACGGGAG AGCTGCGTCT GCGGTGGCCG TGTGCTCCTT CCTGTGCTTC 
TGCCGCCTGT TCAGCACCGC GGAGGCTGCC GTGTACATGT TCAGCATGAA GCGCTGCCCG 
CCAGGCATTT GGCCATCCCA CAAGAGGTAC ATCGAGTACA TGTGTGACAT GGTGGCCGAG 
GAGCCCATCA CACCCCATAG CAAGCCCATC CTGGTGAAAG CCGTGGTCAT GACACCCGTG 
CCCCTGTTCA GCAAGCAGAG AAACGGCTGC AGGCCCTTCT GCGAGGTCTA CGTGGGGGAT 
GAGCGCGTGA CTACCACGTC TCAGGAGTAC GACAAGATGA GGGATTTCAG AATCGAGGAT 
GGCAAAGCAG TCATTCCCCT GGGCATAACA GTGCAAGGGG ACGTGCTCAT TGTCATCTAC 
CACGCCAGGT CCACCCTGGG AGGAAGACTG CAGGCCAAGG TGGCATCCAT GAAGATGTTC 
CAGGTCCAGT TCCACACGGG GTTTGTGCCT CGGAATGCGA CTACTGTGAA GTTTGCAAAG 
TATGACCTTG ATGCGTGTGA CATTCAGGAG AAATACCCAG ATTTATTCCA AGTGAACGTG 
GAGGTGGAGG TGGAGCCCAG AGACAGGCCC AGCCGGGAGG CCCCACCCTG GGAGAGCACC 
AGCATGAGGG GGCTCAACCC CAAAATCCTG TTTTCCAGCC GAGAGGAGCA GCAAGACATT 
CTGTCTAAAT TTGGGAAGCC GGAGCTCCCC AGGCAGCCCG GCTCCACCGC TCAGTACGAC 
GCCGAGGCAG GGTCCCTGGA CGCAGAGCCC ACAGAGTCTG ATTCACCACA GAGCAGCGGC 
ACTGACACCA GCCACTTCCT CCACACGCTG GATTGGCACG AGGAGAAAGA TACAGAGACG 
AGTCCAGAAA GTCACTTTGC CAAGGAGAAC CTATCAACCC TGGCCGAGGA TGCAGGTGGG 
AGTGAGCCTT CGGACGAGGA AGCAGCCACA CCATCCACCG AGAGCAGGGA TGCTGCGGAT 
GAGGACACAC CAGGGCCAGC GGCGAGGCTG CCAGAGCGAG ATTTGATTTT TGATGGGGCC 
ACGCCGGCCA CACCGCAGGA GCCCACACGG CAGGAGGACA GTGTCGACCT CCTAGGGCTG 
CATGCCGAGG CTGGGCTGGC CCCCCCTGTG CAGGCCAGTG GGGCAGCCCC CAGCACCGCC 
GACCTGCTCA GCCGCCTCCT GGGGGGCCCT GACGCTGCTC CGGAGGTGCC CCCGGGTGAC 
CTGCTGGGTG GAGAGGCTCC CCTCCTCTTC GCAAGCCCAG CCCCCTCCTC GAGCGCACAG 
AGCCCGCCCC GTGAGGGGCC AGCTGCCGAC CCCTTTGACC CTCTCCTGCT GCCATCTGGG 
CCCAACACCC AGCCGTGCTC CCAGCCTGAC CTGTTCGGTG AATTTCTTCA TTCGGAATCT 
CCTGCCGCCG TGCCTGCGTC CTTCCCGTCC ACCCACAGCG CCCCACCGCC CGCCTGCAGC 
ACCGACTTCC TGCACCTGGG AGATCTACCA GCAGAGCCTG GCAAGATGAC GGCCTCCACC 
AGCCACCCGG ATCTGCTGGG GGGGTGGGAG GCCTGGGCCG AGGCGCCGGC CCCCGCAGCT 
GCTGCAGAAG GTCCCTTCTT CTCTGCAGGA GGCCAGCCTG CCGCCCCCAG TGCCCCCGGG 
AGCTGGACCA AGTCTCAGAG CCTGGACCCG TTTGCTGACC TCGGTGACCT CAGTTCTGGC 
CTCCAAGGCT CACCGGCTGG ATTTGCTCCA GGGGCTTTTG GCGCCAAGAC GGCCCCCCCT 
CCCACGGGCG GCAGCTCCTG GCAGCAGACG GGTCGGCCCC CGGCCCAGGG CACCCCGTGG 
CCCCCCCAGG CCAAGCCGCC CCCCAAGGCC TGTCCGCAGC CAAGGCCTAA CTATGCTGCC 
AACGTGAGCG TGATTGGCGG CCGAGAGGAG AGAGGGGTCC GGGCCCCCAG CTTCGGCCAG 
AAGCCGAGAG TGTCCGAGAG CGACTTTGAG GACCTGCTGT CCCACCAGGG CTTCTCGTCC 
AAGGCTGACC GGAAGGGGCC AAGGACCATG GCTGAGATGC GGCGGCAGGA GCAGGCGAGG 
GACGCAGACC CGCTGAAGCT GAAGCTCCTG GAGTGGACAG AGGGCAAGGA GAGGAACATC 
CGCGCGCTGC TGTCGACCCT GCACACCGTG CTGTGGGACG GCGAGAGCCG CTGGGCCCCC 
GTGGGCATGG CCGACCTGGT GACCCCCGGG CAGGTGAAGA AGCAGTACCG CCGCGCCGTG 
CTGGTGGTGC ACCCGGACAA GGCCGCAGGG CAGCCCTACG AGCAGTATGC CAAGATGATC 
TTCATGGAGC TGAACGACGC GTGGGCCGAG TTTGAGAGCC AGGGCTCCCG GCCGCTCTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAK ( XM_005618685.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005618742.1 Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X1, mRNA.

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CloneID	OCe04650
Clone ID Related Accession (Same CDS sequence)	XM_005618685.2 , XM_005618685.3
Accession Version	XM_005618685.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	cyclin-G-associated kinase isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005618685.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGTTT CCCCCAAGGC CGGGGTGAAG AGCGCAGGTT TGGGTGCGAT CCGGTACGCA 
GAGGCGGGGG CCGCTGACGC CCGGATGCTG TCGGCCGCCC CGGGGCGCTG CAGCCGTCGC 
CGCCGTCGGG GCCTGTGCTC CGACAACACC GAACACTTGT GCCGGGTTTT TAATACGAGA 
TTCACGGTGG ACAGAGGGTT TGCATTTGTG TATGAAGCTC AAGATCTGGC AAGTGGCAGA 
GAATATGCCT TAAAGAGATT ATTGTCTAAC GAAGAGGAAA AGAACAGAGC CATCATTCAG 
GAAGTTTGTT TCATGAAAAA ACTTTCTGGC CACCCCAATA TTGTCCAGTT TTGTTCTGCA 
GCGTCCATAG GGAAAGAGGA GTCGGACACT GGGCAGGCTG AGTTCCTGCT GCTCACCGAG 
CTCTGCAAAG GGCAGCTGGT GGACTTTTTA AAGAAAATTG AATCTAGAGG TCCGCTCTCG 
TGCGATACTG TTCTGAAGAT TTTCTATCAG ACGTGCAGAG CGGTGCAGCA CATGCACAGA 
CAAAAGCCGC CCATCATCCA TAGAGACCTC AAGGTGGAAA ACTTATTGCT CAGTAACCAA 
GGGACCATCA AGCTGTGTGA CTTTGGCAGT GCCACGACCA TATCACATTA CCCCGACTAC 
AGCTGGAGCG CCCAGAGGCG GGCCCTGGTG GAGGAAGAGA TCACCAGGAA CACCACACCC 
ATGTACAGGA CACCGGAAAT CGTGGACTTG TACTCCAACT TCCCCATCGG CGAGAAGCAG 
GACATCTGGG CGCTGGGCTG CATCTTGTAT CTGCTGTGCT TCCGGCAGCA TCCTTTTGAA 
GACGGAGCAA AGCTTCGGAT AGTCAACGGG AAGTTCTCGA TCCCCCCCAA TGACACCCGG 
TACTCTGTGT TCCACGACCT CATCCGCGCC ACGCTGAAGG TCAACCCCGA GGAGCGGCTG 
TCCATCACAG AACTGGTGAA CCAGCTCCAG GAGATCGCGG CCGCCAGGAA CGTGAACCCC 
AAGTCTCCCA TCACTGAGCT CCTAGAGCAG AACGGAGGCT ATGGGAACGC TGTCCCTTCC 
CGAGGGCCAC CCCCTCCTGG GGGCTCCAGC AGCGGCTACA GCGGAGGCCT GGCGCTGGCC 
GAGTACGACC AGCCCTACGG CGGGCTCCTG GACATCCTGC GGGGGGGCAC GGAGCGCCTC 
TTCACAAACC TCAAGGACAC CTCCTCCAAG GTCATCCAGT CTGTTGCCAA TTACGCAAAA 
GGAGATTTGG ACATCTCTTA CATCACATCT AGAATTGCTG TGATGTCGTT CCCAGCAGAA 
GGCGTGGAGT CGGCCATCAA GAACAACATC GAAGACGTGC GGTTGTTTCT GGACTCCAAG 
CACCCGGGAC ATTACGCCGT CTACAACCTG TCCCCAAGAA CATACCGGCC CTCCAAATTT 
CACAACCGGG TCTCCGAGTG TGGCTGGGCG GCCAGACGGG CCCCGAACCT TCGCAGCCTG 
TACAGTGTGT GTCGGAACAT GCACTCGTGG CTCCGGCAGG ACCACAAGAA CGTCTGTGTC 
GTGCACTGCA TGGACGGGAG AGCTGCGTCT GCGGTGGCCG TGTGCTCCTT CCTGTGCTTC 
TGCCGCCTGT TCAGCACCGC GGAGGCTGCC GTGTACATGT TCAGCATGAA GCGCTGCCCG 
CCAGGCATTT GGCCATCCCA CAAGAGGTAC ATCGAGTACA TGTGTGACAT GGTGGCCGAG 
GAGCCCATCA CACCCCATAG CAAGCCCATC CTGGTGAAAG CCGTGGTCAT GACACCCGTG 
CCCCTGTTCA GCAAGCAGAG AAACGGCTGC AGGCCCTTCT GCGAGGTCTA CGTGGGGGAT 
GAGCGCGTGA CTACCACGTC TCAGGAGTAC GACAAGATGA GGGATTTCAG AATCGAGGAT 
GGCAAAGCAG TCATTCCCCT GGGCATAACA GTGCAAGGGG ACGTGCTCAT TGTCATCTAC 
CACGCCAGGT CCACCCTGGG AGGAAGACTG CAGGCCAAGG TGGCATCCAT GAAGATGTTC 
CAGGTCCAGT TCCACACGGG GTTTGTGCCT CGGAATGCGA CTACTGTGAA GTTTGCAAAG 
TATGACCTTG ATGCGTGTGA CATTCAGGAG AAATACCCAG ATTTATTCCA AGTGAACGTG 
GAGGTGGAGG TGGAGCCCAG AGACAGGCCC AGCCGGGAGG CCCCACCCTG GGAGAGCACC 
AGCATGAGGG GGCTCAACCC CAAAATCCTG TTTTCCAGCC GAGAGGAGCA GCAAGACATT 
CTGTCTAAAT TTGGGAAGCC GGAGCTCCCC AGGCAGCCCG GCTCCACCGC TCAGTACGAC 
GCCGAGGCAG GGTCCCTGGA CGCAGAGCCC ACAGAGTCTG ATTCACCACA GAGCAGCGGC 
ACTGACACCA GCCACTTCCT CCACACGCTG GATTGGCACG AGGAGAAAGA TACAGAGACG 
AGTCCAGAAA GTCACTTTGC CAAGGAGAAC CTATCAACCC TGGCCGAGGA TGCAGGTGGG 
AGTGAGCCTT CGGACGAGGA AGCAGCCACA CCATCCACCG AGAGCAGGGA TGCTGCGGAT 
GAGGACACAC CAGGGCCAGC GGCGAGGCTG CCAGAGCGAG ATTTGATTTT TGATGGGGCC 
ACGCCGGCCA CACCGCAGGA GCCCACACGG CAGGAGGACA GTGTCGACCT CCTAGGGCTG 
CATGCCGAGG CTGGGCTGGC CCCCCCTGTG CAGGCCAGTG GGGCAGCCCC CAGCACCGCC 
GACCTGCTCA GCCGCCTCCT GGGGGGCCCT GACGCTGCTC CGGAGGTGCC CCCGGGTGAC 
CTGCTGGGTG GAGAGGCTCC CCTCCTCTTC GCAAGCCCAG CCCCCTCCTC GAGCGCACAG 
AGCCCGCCCC GTGAGGGGCC AGCTGCCGAC CCCTTTGACC CTCTCCTGCT GCCATCTGGG 
CCCAACACCC AGCCGTGCTC CCAGCCTGAC CTGTTCGGTG AATTTCTTCA TTCGGAATCT 
CCTGCCGCCG TGCCTGCGTC CTTCCCGTCC ACCCACAGCG CCCCACCGCC CGCCTGCAGC 
ACCGACTTCC TGCACCTGGG AGATCTACCA GCAGAGCCTG GCAAGATGAC GGCCTCCACC 
AGCCACCCGG ATCTGCTGGG GGGGTGGGAG GCCTGGGCCG AGGCGCCGGC CCCCGCAGCT 
GCTGCAGAAG GAGGCCAGCC TGCCGCCCCC AGTGCCCCCG GGAGCTGGAC CAAGTCTCAG 
AGCCTGGACC CGTTTGCTGA CCTCGGTGAC CTCAGTTCTG GCCTCCAAGG CTCACCGGCT 
GGATTTGCTC CAGGGGCTTT TGGCGCCAAG ACGGCCCCCC CTCCCACGGG CGGCAGCTCC 
TGGCAGCAGA CGGGTCGGCC CCCGGCCCAG GGCACCCCGT GGCCCCCCCA GGCCAAGCCG 
CCCCCCAAGG CCTGTCCGCA GCCAAGGCCT AACTATGCTG CCAACGTGAG CGTGATTGGC 
GGCCGAGAGG AGAGAGGGGT CCGGGCCCCC AGCTTCGGCC AGAAGCCGAG AGTGTCCGAG 
AGCGACTTTG AGGACCTGCT GTCCCACCAG GGCTTCTCGT CCAAGGCTGA CCGGAAGGGG 
CCAAGGACCA TGGCTGAGAT GCGGCGGCAG GAGCAGGCGA GGGACGCAGA CCCGCTGAAG 
CTGAAGCTCC TGGAGTGGAC AGAGGGCAAG GAGAGGAACA TCCGCGCGCT GCTGTCGACC 
CTGCACACCG TGCTGTGGGA CGGCGAGAGC CGCTGGGCCC CCGTGGGCAT GGCCGACCTG 
GTGACCCCCG GGCAGGTGAA GAAGCAGTAC CGCCGCGCCG TGCTGGTGGT GCACCCGGAC 
AAGGCCGCAG GGCAGCCCTA CGAGCAGTAT GCCAAGATGA TCTTCATGGA GCTGAACGAC 
GCGTGGGCCG AGTTTGAGAG CCAGGGCTCC CGGCCGCTCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005618742.1
CDS	16..3960
Translation	MRVSPKAGVKSAGLGAIRYAEAGAADARMLSAAPGRCSRRRRRGLCSDNTEHLCRVFNTRFTVDRGFAFVYEAQDLASGREYALKRLLSNEEEKNRAIIQEVCFMKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLTELCKGQLVDFLKKIESRGPLSCDTVLKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKFSIPPNDTRYSVFHDLIRATLKVNPEERLSITELVNQLQEIAAARNVNPKSPITELLEQNGGYGNAVPSRGPPPPGGSSSGYSGGLALAEYDQPYGGLLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRLFLDSKHPGHYAVYNLSPRTYRPSKFHNRVSECGWAARRAPNLRSLYSVCRNMHSWLRQDHKNVCVVHCMDGRAASAVAVCSFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYMCDMVAEEPITPHSKPILVKAVVMTPVPLFSKQRNGCRPFCEVYVGDERVTTTSQEYDKMRDFRIEDGKAVIPLGITVQGDVLIVIYHARSTLGGRLQAKVASMKMFQVQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNVEVEVEPRDRPSREAPPWESTSMRGLNPKILFSSREEQQDILSKFGKPELPRQPGSTAQYDAEAGSLDAEPTESDSPQSSGTDTSHFLHTLDWHEEKDTETSPESHFAKENLSTLAEDAGGSEPSDEEAATPSTESRDAADEDTPGPAARLPERDLIFDGATPATPQEPTRQEDSVDLLGLHAEAGLAPPVQASGAAPSTADLLSRLLGGPDAAPEVPPGDLLGGEAPLLFASPAPSSSAQSPPREGPAADPFDPLLLPSGPNTQPCSQPDLFGEFLHSESPAAVPASFPSTHSAPPPACSTDFLHLGDLPAEPGKMTASTSHPDLLGGWEAWAEAPAPAAAAEGGQPAAPSAPGSWTKSQSLDPFADLGDLSSGLQGSPAGFAPGAFGAKTAPPPTGGSSWQQTGRPPAQGTPWPPQAKPPPKACPQPRPNYAANVSVIGGREERGVRAPSFGQKPRVSESDFEDLLSHQGFSSKADRKGPRTMAEMRRQEQARDADPLKLKLLEWTEGKERNIRALLSTLHTVLWDGESRWAPVGMADLVTPGQVKKQYRRAVLVVHPDKAAGQPYEQYAKMIFMELNDAWAEFESQGSRPLF

Target ORF information:

RefSeq Version	XM_005618685.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005618685.2

ORF Insert Sequence:

ATGCGTGTTT CCCCCAAGGC CGGGGTGAAG AGCGCAGGTT TGGGTGCGAT CCGGTACGCA 
GAGGCGGGGG CCGCTGACGC CCGGATGCTG TCGGCCGCCC CGGGGCGCTG CAGCCGTCGC 
CGCCGTCGGG GCCTGTGCTC CGACAACACC GAACACTTGT GCCGGGTTTT TAATACGAGA 
TTCACGGTGG ACAGAGGGTT TGCATTTGTG TATGAAGCTC AAGATCTGGC AAGTGGCAGA 
GAATATGCCT TAAAGAGATT ATTGTCTAAC GAAGAGGAAA AGAACAGAGC CATCATTCAG 
GAAGTTTGTT TCATGAAAAA ACTTTCTGGC CACCCCAATA TTGTCCAGTT TTGTTCTGCA 
GCGTCCATAG GGAAAGAGGA GTCGGACACT GGGCAGGCTG AGTTCCTGCT GCTCACCGAG 
CTCTGCAAAG GGCAGCTGGT GGACTTTTTA AAGAAAATTG AATCTAGAGG TCCGCTCTCG 
TGCGATACTG TTCTGAAGAT TTTCTATCAG ACGTGCAGAG CGGTGCAGCA CATGCACAGA 
CAAAAGCCGC CCATCATCCA TAGAGACCTC AAGGTGGAAA ACTTATTGCT CAGTAACCAA 
GGGACCATCA AGCTGTGTGA CTTTGGCAGT GCCACGACCA TATCACATTA CCCCGACTAC 
AGCTGGAGCG CCCAGAGGCG GGCCCTGGTG GAGGAAGAGA TCACCAGGAA CACCACACCC 
ATGTACAGGA CACCGGAAAT CGTGGACTTG TACTCCAACT TCCCCATCGG CGAGAAGCAG 
GACATCTGGG CGCTGGGCTG CATCTTGTAT CTGCTGTGCT TCCGGCAGCA TCCTTTTGAA 
GACGGAGCAA AGCTTCGGAT AGTCAACGGG AAGTTCTCGA TCCCCCCCAA TGACACCCGG 
TACTCTGTGT TCCACGACCT CATCCGCGCC ACGCTGAAGG TCAACCCCGA GGAGCGGCTG 
TCCATCACAG AACTGGTGAA CCAGCTCCAG GAGATCGCGG CCGCCAGGAA CGTGAACCCC 
AAGTCTCCCA TCACTGAGCT CCTAGAGCAG AACGGAGGCT ATGGGAACGC TGTCCCTTCC 
CGAGGGCCAC CCCCTCCTGG GGGCTCCAGC AGCGGCTACA GCGGAGGCCT GGCGCTGGCC 
GAGTACGACC AGCCCTACGG CGGGCTCCTG GACATCCTGC GGGGGGGCAC GGAGCGCCTC 
TTCACAAACC TCAAGGACAC CTCCTCCAAG GTCATCCAGT CTGTTGCCAA TTACGCAAAA 
GGAGATTTGG ACATCTCTTA CATCACATCT AGAATTGCTG TGATGTCGTT CCCAGCAGAA 
GGCGTGGAGT CGGCCATCAA GAACAACATC GAAGACGTGC GGTTGTTTCT GGACTCCAAG 
CACCCGGGAC ATTACGCCGT CTACAACCTG TCCCCAAGAA CATACCGGCC CTCCAAATTT 
CACAACCGGG TCTCCGAGTG TGGCTGGGCG GCCAGACGGG CCCCGAACCT TCGCAGCCTG 
TACAGTGTGT GTCGGAACAT GCACTCGTGG CTCCGGCAGG ACCACAAGAA CGTCTGTGTC 
GTGCACTGCA TGGACGGGAG AGCTGCGTCT GCGGTGGCCG TGTGCTCCTT CCTGTGCTTC 
TGCCGCCTGT TCAGCACCGC GGAGGCTGCC GTGTACATGT TCAGCATGAA GCGCTGCCCG 
CCAGGCATTT GGCCATCCCA CAAGAGGTAC ATCGAGTACA TGTGTGACAT GGTGGCCGAG 
GAGCCCATCA CACCCCATAG CAAGCCCATC CTGGTGAAAG CCGTGGTCAT GACACCCGTG 
CCCCTGTTCA GCAAGCAGAG AAACGGCTGC AGGCCCTTCT GCGAGGTCTA CGTGGGGGAT 
GAGCGCGTGA CTACCACGTC TCAGGAGTAC GACAAGATGA GGGATTTCAG AATCGAGGAT 
GGCAAAGCAG TCATTCCCCT GGGCATAACA GTGCAAGGGG ACGTGCTCAT TGTCATCTAC 
CACGCCAGGT CCACCCTGGG AGGAAGACTG CAGGCCAAGG TGGCATCCAT GAAGATGTTC 
CAGGTCCAGT TCCACACGGG GTTTGTGCCT CGGAATGCGA CTACTGTGAA GTTTGCAAAG 
TATGACCTTG ATGCGTGTGA CATTCAGGAG AAATACCCAG ATTTATTCCA AGTGAACGTG 
GAGGTGGAGG TGGAGCCCAG AGACAGGCCC AGCCGGGAGG CCCCACCCTG GGAGAGCACC 
AGCATGAGGG GGCTCAACCC CAAAATCCTG TTTTCCAGCC GAGAGGAGCA GCAAGACATT 
CTGTCTAAAT TTGGGAAGCC GGAGCTCCCC AGGCAGCCCG GCTCCACCGC TCAGTACGAC 
GCCGAGGCAG GGTCCCTGGA CGCAGAGCCC ACAGAGTCTG ATTCACCACA GAGCAGCGGC 
ACTGACACCA GCCACTTCCT CCACACGCTG GATTGGCACG AGGAGAAAGA TACAGAGACG 
AGTCCAGAAA GTCACTTTGC CAAGGAGAAC CTATCAACCC TGGCCGAGGA TGCAGGTGGG 
AGTGAGCCTT CGGACGAGGA AGCAGCCACA CCATCCACCG AGAGCAGGGA TGCTGCGGAT 
GAGGACACAC CAGGGCCAGC GGCGAGGCTG CCAGAGCGAG ATTTGATTTT TGATGGGGCC 
ACGCCGGCCA CACCGCAGGA GCCCACACGG CAGGAGGACA GTGTCGACCT CCTAGGGCTG 
CATGCCGAGG CTGGGCTGGC CCCCCCTGTG CAGGCCAGTG GGGCAGCCCC CAGCACCGCC 
GACCTGCTCA GCCGCCTCCT GGGGGGCCCT GACGCTGCTC CGGAGGTGCC CCCGGGTGAC 
CTGCTGGGTG GAGAGGCTCC CCTCCTCTTC GCAAGCCCAG CCCCCTCCTC GAGCGCACAG 
AGCCCGCCCC GTGAGGGGCC AGCTGCCGAC CCCTTTGACC CTCTCCTGCT GCCATCTGGG 
CCCAACACCC AGCCGTGCTC CCAGCCTGAC CTGTTCGGTG AATTTCTTCA TTCGGAATCT 
CCTGCCGCCG TGCCTGCGTC CTTCCCGTCC ACCCACAGCG CCCCACCGCC CGCCTGCAGC 
ACCGACTTCC TGCACCTGGG AGATCTACCA GCAGAGCCTG GCAAGATGAC GGCCTCCACC 
AGCCACCCGG ATCTGCTGGG GGGGTGGGAG GCCTGGGCCG AGGCGCCGGC CCCCGCAGCT 
GCTGCAGAAG GAGGCCAGCC TGCCGCCCCC AGTGCCCCCG GGAGCTGGAC CAAGTCTCAG 
AGCCTGGACC CGTTTGCTGA CCTCGGTGAC CTCAGTTCTG GCCTCCAAGG CTCACCGGCT 
GGATTTGCTC CAGGGGCTTT TGGCGCCAAG ACGGCCCCCC CTCCCACGGG CGGCAGCTCC 
TGGCAGCAGA CGGGTCGGCC CCCGGCCCAG GGCACCCCGT GGCCCCCCCA GGCCAAGCCG 
CCCCCCAAGG CCTGTCCGCA GCCAAGGCCT AACTATGCTG CCAACGTGAG CGTGATTGGC 
GGCCGAGAGG AGAGAGGGGT CCGGGCCCCC AGCTTCGGCC AGAAGCCGAG AGTGTCCGAG 
AGCGACTTTG AGGACCTGCT GTCCCACCAG GGCTTCTCGT CCAAGGCTGA CCGGAAGGGG 
CCAAGGACCA TGGCTGAGAT GCGGCGGCAG GAGCAGGCGA GGGACGCAGA CCCGCTGAAG 
CTGAAGCTCC TGGAGTGGAC AGAGGGCAAG GAGAGGAACA TCCGCGCGCT GCTGTCGACC 
CTGCACACCG TGCTGTGGGA CGGCGAGAGC CGCTGGGCCC CCGTGGGCAT GGCCGACCTG 
GTGACCCCCG GGCAGGTGAA GAAGCAGTAC CGCCGCGCCG TGCTGGTGGT GCACCCGGAC 
AAGGCCGCAG GGCAGCCCTA CGAGCAGTAT GCCAAGATGA TCTTCATGGA GCTGAACGAC 
GCGTGGGCCG AGTTTGAGAG CCAGGGCTCC CGGCCGCTCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAK ( XM_005618685.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005618742.1 Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X2, mRNA.

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CloneID	OCe04650
Clone ID Related Accession (Same CDS sequence)	XM_005618685.2 , XM_005618685.3
Accession Version	XM_005618685.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	cyclin-G-associated kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005618685.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGTTT CCCCCAAGGC CGGGGTGAAG AGCGCAGGTT TGGGTGCGAT CCGGTACGCA 
GAGGCGGGGG CCGCTGACGC CCGGATGCTG TCGGCCGCCC CGGGGCGCTG CAGCCGTCGC 
CGCCGTCGGG GCCTGTGCTC CGACAACACC GAACACTTGT GCCGGGTTTT TAATACGAGA 
TTCACGGTGG ACAGAGGGTT TGCATTTGTG TATGAAGCTC AAGATCTGGC AAGTGGCAGA 
GAATATGCCT TAAAGAGATT ATTGTCTAAC GAAGAGGAAA AGAACAGAGC CATCATTCAG 
GAAGTTTGTT TCATGAAAAA ACTTTCTGGC CACCCCAATA TTGTCCAGTT TTGTTCTGCA 
GCGTCCATAG GGAAAGAGGA GTCGGACACT GGGCAGGCTG AGTTCCTGCT GCTCACCGAG 
CTCTGCAAAG GGCAGCTGGT GGACTTTTTA AAGAAAATTG AATCTAGAGG TCCGCTCTCG 
TGCGATACTG TTCTGAAGAT TTTCTATCAG ACGTGCAGAG CGGTGCAGCA CATGCACAGA 
CAAAAGCCGC CCATCATCCA TAGAGACCTC AAGGTGGAAA ACTTATTGCT CAGTAACCAA 
GGGACCATCA AGCTGTGTGA CTTTGGCAGT GCCACGACCA TATCACATTA CCCCGACTAC 
AGCTGGAGCG CCCAGAGGCG GGCCCTGGTG GAGGAAGAGA TCACCAGGAA CACCACACCC 
ATGTACAGGA CACCGGAAAT CGTGGACTTG TACTCCAACT TCCCCATCGG CGAGAAGCAG 
GACATCTGGG CGCTGGGCTG CATCTTGTAT CTGCTGTGCT TCCGGCAGCA TCCTTTTGAA 
GACGGAGCAA AGCTTCGGAT AGTCAACGGG AAGTTCTCGA TCCCCCCCAA TGACACCCGG 
TACTCTGTGT TCCACGACCT CATCCGCGCC ACGCTGAAGG TCAACCCCGA GGAGCGGCTG 
TCCATCACAG AACTGGTGAA CCAGCTCCAG GAGATCGCGG CCGCCAGGAA CGTGAACCCC 
AAGTCTCCCA TCACTGAGCT CCTAGAGCAG AACGGAGGCT ATGGGAACGC TGTCCCTTCC 
CGAGGGCCAC CCCCTCCTGG GGGCTCCAGC AGCGGCTACA GCGGAGGCCT GGCGCTGGCC 
GAGTACGACC AGCCCTACGG CGGGCTCCTG GACATCCTGC GGGGGGGCAC GGAGCGCCTC 
TTCACAAACC TCAAGGACAC CTCCTCCAAG GTCATCCAGT CTGTTGCCAA TTACGCAAAA 
GGAGATTTGG ACATCTCTTA CATCACATCT AGAATTGCTG TGATGTCGTT CCCAGCAGAA 
GGCGTGGAGT CGGCCATCAA GAACAACATC GAAGACGTGC GGTTGTTTCT GGACTCCAAG 
CACCCGGGAC ATTACGCCGT CTACAACCTG TCCCCAAGAA CATACCGGCC CTCCAAATTT 
CACAACCGGG TCTCCGAGTG TGGCTGGGCG GCCAGACGGG CCCCGAACCT TCGCAGCCTG 
TACAGTGTGT GTCGGAACAT GCACTCGTGG CTCCGGCAGG ACCACAAGAA CGTCTGTGTC 
GTGCACTGCA TGGACGGGAG AGCTGCGTCT GCGGTGGCCG TGTGCTCCTT CCTGTGCTTC 
TGCCGCCTGT TCAGCACCGC GGAGGCTGCC GTGTACATGT TCAGCATGAA GCGCTGCCCG 
CCAGGCATTT GGCCATCCCA CAAGAGGTAC ATCGAGTACA TGTGTGACAT GGTGGCCGAG 
GAGCCCATCA CACCCCATAG CAAGCCCATC CTGGTGAAAG CCGTGGTCAT GACACCCGTG 
CCCCTGTTCA GCAAGCAGAG AAACGGCTGC AGGCCCTTCT GCGAGGTCTA CGTGGGGGAT 
GAGCGCGTGA CTACCACGTC TCAGGAGTAC GACAAGATGA GGGATTTCAG AATCGAGGAT 
GGCAAAGCAG TCATTCCCCT GGGCATAACA GTGCAAGGGG ACGTGCTCAT TGTCATCTAC 
CACGCCAGGT CCACCCTGGG AGGAAGACTG CAGGCCAAGG TGGCATCCAT GAAGATGTTC 
CAGGTCCAGT TCCACACGGG GTTTGTGCCT CGGAATGCGA CTACTGTGAA GTTTGCAAAG 
TATGACCTTG ATGCGTGTGA CATTCAGGAG AAATACCCAG ATTTATTCCA AGTGAACGTG 
GAGGTGGAGG TGGAGCCCAG AGACAGGCCC AGCCGGGAGG CCCCACCCTG GGAGAGCACC 
AGCATGAGGG GGCTCAACCC CAAAATCCTG TTTTCCAGCC GAGAGGAGCA GCAAGACATT 
CTGTCTAAAT TTGGGAAGCC GGAGCTCCCC AGGCAGCCCG GCTCCACCGC TCAGTACGAC 
GCCGAGGCAG GGTCCCTGGA CGCAGAGCCC ACAGAGTCTG ATTCACCACA GAGCAGCGGC 
ACTGACACCA GCCACTTCCT CCACACGCTG GATTGGCACG AGGAGAAAGA TACAGAGACG 
AGTCCAGAAA GTCACTTTGC CAAGGAGAAC CTATCAACCC TGGCCGAGGA TGCAGGTGGG 
AGTGAGCCTT CGGACGAGGA AGCAGCCACA CCATCCACCG AGAGCAGGGA TGCTGCGGAT 
GAGGACACAC CAGGGCCAGC GGCGAGGCTG CCAGAGCGAG ATTTGATTTT TGATGGGGCC 
ACGCCGGCCA CACCGCAGGA GCCCACACGG CAGGAGGACA GTGTCGACCT CCTAGGGCTG 
CATGCCGAGG CTGGGCTGGC CCCCCCTGTG CAGGCCAGTG GGGCAGCCCC CAGCACCGCC 
GACCTGCTCA GCCGCCTCCT GGGGGGCCCT GACGCTGCTC CGGAGGTGCC CCCGGGTGAC 
CTGCTGGGTG GAGAGGCTCC CCTCCTCTTC GCAAGCCCAG CCCCCTCCTC GAGCGCACAG 
AGCCCGCCCC GTGAGGGGCC AGCTGCCGAC CCCTTTGACC CTCTCCTGCT GCCATCTGGG 
CCCAACACCC AGCCGTGCTC CCAGCCTGAC CTGTTCGGTG AATTTCTTCA TTCGGAATCT 
CCTGCCGCCG TGCCTGCGTC CTTCCCGTCC ACCCACAGCG CCCCACCGCC CGCCTGCAGC 
ACCGACTTCC TGCACCTGGG AGATCTACCA GCAGAGCCTG GCAAGATGAC GGCCTCCACC 
AGCCACCCGG ATCTGCTGGG GGGGTGGGAG GCCTGGGCCG AGGCGCCGGC CCCCGCAGCT 
GCTGCAGAAG GAGGCCAGCC TGCCGCCCCC AGTGCCCCCG GGAGCTGGAC CAAGTCTCAG 
AGCCTGGACC CGTTTGCTGA CCTCGGTGAC CTCAGTTCTG GCCTCCAAGG CTCACCGGCT 
GGATTTGCTC CAGGGGCTTT TGGCGCCAAG ACGGCCCCCC CTCCCACGGG CGGCAGCTCC 
TGGCAGCAGA CGGGTCGGCC CCCGGCCCAG GGCACCCCGT GGCCCCCCCA GGCCAAGCCG 
CCCCCCAAGG CCTGTCCGCA GCCAAGGCCT AACTATGCTG CCAACGTGAG CGTGATTGGC 
GGCCGAGAGG AGAGAGGGGT CCGGGCCCCC AGCTTCGGCC AGAAGCCGAG AGTGTCCGAG 
AGCGACTTTG AGGACCTGCT GTCCCACCAG GGCTTCTCGT CCAAGGCTGA CCGGAAGGGG 
CCAAGGACCA TGGCTGAGAT GCGGCGGCAG GAGCAGGCGA GGGACGCAGA CCCGCTGAAG 
CTGAAGCTCC TGGAGTGGAC AGAGGGCAAG GAGAGGAACA TCCGCGCGCT GCTGTCGACC 
CTGCACACCG TGCTGTGGGA CGGCGAGAGC CGCTGGGCCC CCGTGGGCAT GGCCGACCTG 
GTGACCCCCG GGCAGGTGAA GAAGCAGTAC CGCCGCGCCG TGCTGGTGGT GCACCCGGAC 
AAGGCCGCAG GGCAGCCCTA CGAGCAGTAT GCCAAGATGA TCTTCATGGA GCTGAACGAC 
GCGTGGGCCG AGTTTGAGAG CCAGGGCTCC CGGCCGCTCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005618742.1
CDS	16..3960
Translation	MRVSPKAGVKSAGLGAIRYAEAGAADARMLSAAPGRCSRRRRRGLCSDNTEHLCRVFNTRFTVDRGFAFVYEAQDLASGREYALKRLLSNEEEKNRAIIQEVCFMKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLTELCKGQLVDFLKKIESRGPLSCDTVLKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKFSIPPNDTRYSVFHDLIRATLKVNPEERLSITELVNQLQEIAAARNVNPKSPITELLEQNGGYGNAVPSRGPPPPGGSSSGYSGGLALAEYDQPYGGLLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRLFLDSKHPGHYAVYNLSPRTYRPSKFHNRVSECGWAARRAPNLRSLYSVCRNMHSWLRQDHKNVCVVHCMDGRAASAVAVCSFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYMCDMVAEEPITPHSKPILVKAVVMTPVPLFSKQRNGCRPFCEVYVGDERVTTTSQEYDKMRDFRIEDGKAVIPLGITVQGDVLIVIYHARSTLGGRLQAKVASMKMFQVQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNVEVEVEPRDRPSREAPPWESTSMRGLNPKILFSSREEQQDILSKFGKPELPRQPGSTAQYDAEAGSLDAEPTESDSPQSSGTDTSHFLHTLDWHEEKDTETSPESHFAKENLSTLAEDAGGSEPSDEEAATPSTESRDAADEDTPGPAARLPERDLIFDGATPATPQEPTRQEDSVDLLGLHAEAGLAPPVQASGAAPSTADLLSRLLGGPDAAPEVPPGDLLGGEAPLLFASPAPSSSAQSPPREGPAADPFDPLLLPSGPNTQPCSQPDLFGEFLHSESPAAVPASFPSTHSAPPPACSTDFLHLGDLPAEPGKMTASTSHPDLLGGWEAWAEAPAPAAAAEGGQPAAPSAPGSWTKSQSLDPFADLGDLSSGLQGSPAGFAPGAFGAKTAPPPTGGSSWQQTGRPPAQGTPWPPQAKPPPKACPQPRPNYAANVSVIGGREERGVRAPSFGQKPRVSESDFEDLLSHQGFSSKADRKGPRTMAEMRRQEQARDADPLKLKLLEWTEGKERNIRALLSTLHTVLWDGESRWAPVGMADLVTPGQVKKQYRRAVLVVHPDKAAGQPYEQYAKMIFMELNDAWAEFESQGSRPLF

Target ORF information:

RefSeq Version	XM_005618685.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005618685.3

ORF Insert Sequence:

ATGCGTGTTT CCCCCAAGGC CGGGGTGAAG AGCGCAGGTT TGGGTGCGAT CCGGTACGCA 
GAGGCGGGGG CCGCTGACGC CCGGATGCTG TCGGCCGCCC CGGGGCGCTG CAGCCGTCGC 
CGCCGTCGGG GCCTGTGCTC CGACAACACC GAACACTTGT GCCGGGTTTT TAATACGAGA 
TTCACGGTGG ACAGAGGGTT TGCATTTGTG TATGAAGCTC AAGATCTGGC AAGTGGCAGA 
GAATATGCCT TAAAGAGATT ATTGTCTAAC GAAGAGGAAA AGAACAGAGC CATCATTCAG 
GAAGTTTGTT TCATGAAAAA ACTTTCTGGC CACCCCAATA TTGTCCAGTT TTGTTCTGCA 
GCGTCCATAG GGAAAGAGGA GTCGGACACT GGGCAGGCTG AGTTCCTGCT GCTCACCGAG 
CTCTGCAAAG GGCAGCTGGT GGACTTTTTA AAGAAAATTG AATCTAGAGG TCCGCTCTCG 
TGCGATACTG TTCTGAAGAT TTTCTATCAG ACGTGCAGAG CGGTGCAGCA CATGCACAGA 
CAAAAGCCGC CCATCATCCA TAGAGACCTC AAGGTGGAAA ACTTATTGCT CAGTAACCAA 
GGGACCATCA AGCTGTGTGA CTTTGGCAGT GCCACGACCA TATCACATTA CCCCGACTAC 
AGCTGGAGCG CCCAGAGGCG GGCCCTGGTG GAGGAAGAGA TCACCAGGAA CACCACACCC 
ATGTACAGGA CACCGGAAAT CGTGGACTTG TACTCCAACT TCCCCATCGG CGAGAAGCAG 
GACATCTGGG CGCTGGGCTG CATCTTGTAT CTGCTGTGCT TCCGGCAGCA TCCTTTTGAA 
GACGGAGCAA AGCTTCGGAT AGTCAACGGG AAGTTCTCGA TCCCCCCCAA TGACACCCGG 
TACTCTGTGT TCCACGACCT CATCCGCGCC ACGCTGAAGG TCAACCCCGA GGAGCGGCTG 
TCCATCACAG AACTGGTGAA CCAGCTCCAG GAGATCGCGG CCGCCAGGAA CGTGAACCCC 
AAGTCTCCCA TCACTGAGCT CCTAGAGCAG AACGGAGGCT ATGGGAACGC TGTCCCTTCC 
CGAGGGCCAC CCCCTCCTGG GGGCTCCAGC AGCGGCTACA GCGGAGGCCT GGCGCTGGCC 
GAGTACGACC AGCCCTACGG CGGGCTCCTG GACATCCTGC GGGGGGGCAC GGAGCGCCTC 
TTCACAAACC TCAAGGACAC CTCCTCCAAG GTCATCCAGT CTGTTGCCAA TTACGCAAAA 
GGAGATTTGG ACATCTCTTA CATCACATCT AGAATTGCTG TGATGTCGTT CCCAGCAGAA 
GGCGTGGAGT CGGCCATCAA GAACAACATC GAAGACGTGC GGTTGTTTCT GGACTCCAAG 
CACCCGGGAC ATTACGCCGT CTACAACCTG TCCCCAAGAA CATACCGGCC CTCCAAATTT 
CACAACCGGG TCTCCGAGTG TGGCTGGGCG GCCAGACGGG CCCCGAACCT TCGCAGCCTG 
TACAGTGTGT GTCGGAACAT GCACTCGTGG CTCCGGCAGG ACCACAAGAA CGTCTGTGTC 
GTGCACTGCA TGGACGGGAG AGCTGCGTCT GCGGTGGCCG TGTGCTCCTT CCTGTGCTTC 
TGCCGCCTGT TCAGCACCGC GGAGGCTGCC GTGTACATGT TCAGCATGAA GCGCTGCCCG 
CCAGGCATTT GGCCATCCCA CAAGAGGTAC ATCGAGTACA TGTGTGACAT GGTGGCCGAG 
GAGCCCATCA CACCCCATAG CAAGCCCATC CTGGTGAAAG CCGTGGTCAT GACACCCGTG 
CCCCTGTTCA GCAAGCAGAG AAACGGCTGC AGGCCCTTCT GCGAGGTCTA CGTGGGGGAT 
GAGCGCGTGA CTACCACGTC TCAGGAGTAC GACAAGATGA GGGATTTCAG AATCGAGGAT 
GGCAAAGCAG TCATTCCCCT GGGCATAACA GTGCAAGGGG ACGTGCTCAT TGTCATCTAC 
CACGCCAGGT CCACCCTGGG AGGAAGACTG CAGGCCAAGG TGGCATCCAT GAAGATGTTC 
CAGGTCCAGT TCCACACGGG GTTTGTGCCT CGGAATGCGA CTACTGTGAA GTTTGCAAAG 
TATGACCTTG ATGCGTGTGA CATTCAGGAG AAATACCCAG ATTTATTCCA AGTGAACGTG 
GAGGTGGAGG TGGAGCCCAG AGACAGGCCC AGCCGGGAGG CCCCACCCTG GGAGAGCACC 
AGCATGAGGG GGCTCAACCC CAAAATCCTG TTTTCCAGCC GAGAGGAGCA GCAAGACATT 
CTGTCTAAAT TTGGGAAGCC GGAGCTCCCC AGGCAGCCCG GCTCCACCGC TCAGTACGAC 
GCCGAGGCAG GGTCCCTGGA CGCAGAGCCC ACAGAGTCTG ATTCACCACA GAGCAGCGGC 
ACTGACACCA GCCACTTCCT CCACACGCTG GATTGGCACG AGGAGAAAGA TACAGAGACG 
AGTCCAGAAA GTCACTTTGC CAAGGAGAAC CTATCAACCC TGGCCGAGGA TGCAGGTGGG 
AGTGAGCCTT CGGACGAGGA AGCAGCCACA CCATCCACCG AGAGCAGGGA TGCTGCGGAT 
GAGGACACAC CAGGGCCAGC GGCGAGGCTG CCAGAGCGAG ATTTGATTTT TGATGGGGCC 
ACGCCGGCCA CACCGCAGGA GCCCACACGG CAGGAGGACA GTGTCGACCT CCTAGGGCTG 
CATGCCGAGG CTGGGCTGGC CCCCCCTGTG CAGGCCAGTG GGGCAGCCCC CAGCACCGCC 
GACCTGCTCA GCCGCCTCCT GGGGGGCCCT GACGCTGCTC CGGAGGTGCC CCCGGGTGAC 
CTGCTGGGTG GAGAGGCTCC CCTCCTCTTC GCAAGCCCAG CCCCCTCCTC GAGCGCACAG 
AGCCCGCCCC GTGAGGGGCC AGCTGCCGAC CCCTTTGACC CTCTCCTGCT GCCATCTGGG 
CCCAACACCC AGCCGTGCTC CCAGCCTGAC CTGTTCGGTG AATTTCTTCA TTCGGAATCT 
CCTGCCGCCG TGCCTGCGTC CTTCCCGTCC ACCCACAGCG CCCCACCGCC CGCCTGCAGC 
ACCGACTTCC TGCACCTGGG AGATCTACCA GCAGAGCCTG GCAAGATGAC GGCCTCCACC 
AGCCACCCGG ATCTGCTGGG GGGGTGGGAG GCCTGGGCCG AGGCGCCGGC CCCCGCAGCT 
GCTGCAGAAG GAGGCCAGCC TGCCGCCCCC AGTGCCCCCG GGAGCTGGAC CAAGTCTCAG 
AGCCTGGACC CGTTTGCTGA CCTCGGTGAC CTCAGTTCTG GCCTCCAAGG CTCACCGGCT 
GGATTTGCTC CAGGGGCTTT TGGCGCCAAG ACGGCCCCCC CTCCCACGGG CGGCAGCTCC 
TGGCAGCAGA CGGGTCGGCC CCCGGCCCAG GGCACCCCGT GGCCCCCCCA GGCCAAGCCG 
CCCCCCAAGG CCTGTCCGCA GCCAAGGCCT AACTATGCTG CCAACGTGAG CGTGATTGGC 
GGCCGAGAGG AGAGAGGGGT CCGGGCCCCC AGCTTCGGCC AGAAGCCGAG AGTGTCCGAG 
AGCGACTTTG AGGACCTGCT GTCCCACCAG GGCTTCTCGT CCAAGGCTGA CCGGAAGGGG 
CCAAGGACCA TGGCTGAGAT GCGGCGGCAG GAGCAGGCGA GGGACGCAGA CCCGCTGAAG 
CTGAAGCTCC TGGAGTGGAC AGAGGGCAAG GAGAGGAACA TCCGCGCGCT GCTGTCGACC 
CTGCACACCG TGCTGTGGGA CGGCGAGAGC CGCTGGGCCC CCGTGGGCAT GGCCGACCTG 
GTGACCCCCG GGCAGGTGAA GAAGCAGTAC CGCCGCGCCG TGCTGGTGGT GCACCCGGAC 
AAGGCCGCAG GGCAGCCCTA CGAGCAGTAT GCCAAGATGA TCTTCATGGA GCTGAACGAC 
GCGTGGGCCG AGTTTGAGAG CCAGGGCTCC CGGCCGCTCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAK ( XM_536279.7 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_536279.4 Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe04651
Clone ID Related Accession (Same CDS sequence)	XM_536279.7 , XM_536279.6
Accession Version	XM_536279.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	cyclin-G-associated kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_536279.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTGC TGCAGTCTGC GCTGGACTTC CTGGCGGGCC CGGGCTCCCT GGGCGGCGCC 
GCCGGCCGCG ACCAGAGCGA CTTCGTGGGG CAGACGGTGG AGCTGGGCGA GCTGCGGCTG 
CGGGTGCGGC GGGTCCTGGC GGAGGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATCTG 
GCAAGTGGCA GAGAATATGC CTTAAAGAGA TTATTGTCTA ACGAAGAGGA AAAGAACAGA 
GCCATCATTC AGGAAGTTTG TTTCATGAAA AAACTTTCTG GCCACCCCAA TATTGTCCAG 
TTTTGTTCTG CAGCGTCCAT AGGGAAAGAG GAGTCGGACA CTGGGCAGGC TGAGTTCCTG 
CTGCTCACCG AGCTCTGCAA AGGGCAGCTG GTGGACTTTT TAAAGAAAAT TGAATCTAGA 
GGTCCGCTCT CGTGCGATAC TGTTCTGAAG ATTTTCTATC AGACGTGCAG AGCGGTGCAG 
CACATGCACA GACAAAAGCC GCCCATCATC CATAGAGACC TCAAGGTGGA AAACTTATTG 
CTCAGTAACC AAGGGACCAT CAAGCTGTGT GACTTTGGCA GTGCCACGAC CATATCACAT 
TACCCCGACT ACAGCTGGAG CGCCCAGAGG CGGGCCCTGG TGGAGGAAGA GATCACCAGG 
AACACCACAC CCATGTACAG GACACCGGAA ATCGTGGACT TGTACTCCAA CTTCCCCATC 
GGCGAGAAGC AGGACATCTG GGCGCTGGGC TGCATCTTGT ATCTGCTGTG CTTCCGGCAG 
CATCCTTTTG AAGACGGAGC AAAGCTTCGG ATAGTCAACG GGAAGTTCTC GATCCCCCCC 
AATGACACCC GGTACTCTGT GTTCCACGAC CTCATCCGCG CCACGCTGAA GGTCAACCCC 
GAGGAGCGGC TGTCCATCAC AGAACTGGTG AACCAGCTCC AGGAGATCGC GGCCGCCAGG 
AACGTGAACC CCAAGTCTCC CATCACTGAG CTCCTAGAGC AGAACGGAGG CTATGGGAAC 
GCTGTCCCTT CCCGAGGGCC ACCCCCTCCT GGGGGCTCCA GCAGCGGCTA CAGCGGAGGC 
CTGGCGCTGG CCGAGTACGA CCAGCCCTAC GGCGGGCTCC TGGACATCCT GCGGGGGGGC 
ACGGAGCGCC TCTTCACAAA CCTCAAGGAC ACCTCCTCCA AGGTCATCCA GTCTGTTGCC 
AATTACGCAA AAGGAGATTT GGACATCTCT TACATCACAT CTAGAATTGC TGTGATGTCG 
TTCCCAGCAG AAGGCGTGGA GTCGGCCATC AAGAACAACA TCGAAGACGT GCGGTTGTTT 
CTGGACTCCA AGCACCCGGG ACATTACGCC GTCTACAACC TGTCCCCAAG AACATACCGG 
CCCTCCAAAT TTCACAACCG GGTCTCCGAG TGTGGCTGGG CGGCCAGACG GGCCCCGAAC 
CTTCGCAGCC TGTACAGTGT GTGTCGGAAC ATGCACTCGT GGCTCCGGCA GGACCACAAG 
AACGTCTGTG TCGTGCACTG CATGGACGGG AGAGCTGCGT CTGCGGTGGC CGTGTGCTCC 
TTCCTGTGCT TCTGCCGCCT GTTCAGCACC GCGGAGGCTG CCGTGTACAT GTTCAGCATG 
AAGCGCTGCC CGCCAGGCAT TTGGCCATCC CACAAGAGGT ACATCGAGTA CATGTGTGAC 
ATGGTGGCCG AGGAGCCCAT CACACCCCAT AGCAAGCCCA TCCTGGTGAA AGCCGTGGTC 
ATGACACCCG TGCCCCTGTT CAGCAAGCAG AGAAACGGCT GCAGGCCCTT CTGCGAGGTC 
TACGTGGGGG ATGAGCGCGT GACTACCACG TCTCAGGAGT ACGACAAGAT GAGGGATTTC 
AGAATCGAGG ATGGCAAAGC AGTCATTCCC CTGGGCATAA CAGTGCAAGG GGACGTGCTC 
ATTGTCATCT ACCACGCCAG GTCCACCCTG GGAGGAAGAC TGCAGGCCAA GGTGGCATCC 
ATGAAGATGT TCCAGGTCCA GTTCCACACG GGGTTTGTGC CTCGGAATGC GACTACTGTG 
AAGTTTGCAA AGTATGACCT TGATGCGTGT GACATTCAGG AGAAATACCC AGATTTATTC 
CAAGTGAACG TGGAGGTGGA GGTGGAGCCC AGAGACAGGC CCAGCCGGGA GGCCCCACCC 
TGGGAGAGCA CCAGCATGAG GGGGCTCAAC CCCAAAATCC TGTTTTCCAG CCGAGAGGAG 
CAGCAAGACA TTCTGTCTAA ATTTGGGAAG CCGGAGCTCC CCAGGCAGCC CGGCTCCACC 
GCTCAGTACG ACGCCGAGGC AGGGTCCCTG GACGCAGAGC CCACAGAGTC TGATTCACCA 
CAGAGCAGCG GCACTGACAC CAGCCACTTC CTCCACACGC TGGATTGGCA CGAGGAGAAA 
GATACAGAGA CGAGTCCAGA AAGTCACTTT GCCAAGGAGA ACCTATCAAC CCTGGCCGAG 
GATGCAGGTG GGAGTGAGCC TTCGGACGAG GAAGCAGCCA CACCATCCAC CGAGAGCAGG 
GATGCTGCGG ATGAGGACAC ACCAGGGCCA GCGGCGAGGC TGCCAGAGCG AGATTTGATT 
TTTGATGGGG CCACGCCGGC CACACCGCAG GAGCCCACAC GGCAGGAGGA CAGTGTCGAC 
CTCCTAGGGC TGCATGCCGA GGCTGGGCTG GCCCCCCCTG TGCAGGCCAG TGGGGCAGCC 
CCCAGCACCG CCGACCTGCT CAGCCGCCTC CTGGGGGGCC CTGACGCTGC TCCGGAGGTG 
CCCCCGGGTG ACCTGCTGGG TGGAGAGGCT CCCCTCCTCT TCGCAAGCCC AGCCCCCTCC 
TCGAGCGCAC AGAGCCCGCC CCGTGAGGGG CCAGCTGCCG ACCCCTTTGA CCCTCTCCTG 
CTGCCATCTG GGCCCAACAC CCAGCCGTGC TCCCAGCCTG ACCTGTTCGG TGAATTTCTT 
CATTCGGAAT CTCCTGCCGC CGTGCCTGCG TCCTTCCCGT CCACCCACAG CGCCCCACCG 
CCCGCCTGCA GCACCGACTT CCTGCACCTG GGAGATCTAC CAGCAGAGCC TGGCAAGATG 
ACGGCCTCCA CCAGCCACCC GGATCTGCTG GGGGGGTGGG AGGCCTGGGC CGAGGCGCCG 
GCCCCCGCAG CTGCTGCAGA AGGTCCCTTC TTCTCTGCAG GAGGCCAGCC TGCCGCCCCC 
AGTGCCCCCG GGAGCTGGAC CAAGTCTCAG AGCCTGGACC CGTTTGCTGA CCTCGGTGAC 
CTCAGTTCTG GCCTCCAAGG CTCACCGGCT GGATTTGCTC CAGGGGCTTT TGGCGCCAAG 
ACGGCCCCCC CTCCCACGGG CGGCAGCTCC TGGCAGCAGA CGGGTCGGCC CCCGGCCCAG 
GGCACCCCGT GGCCCCCCCA GGCCAAGCCG CCCCCCAAGG CCTGTCCGCA GCCAAGGCCT 
AACTATGCTG CCAACGTGAG CGTGATTGGC GGCCGAGAGG AGAGAGGGGT CCGGGCCCCC 
AGCTTCGGCC AGAAGCCGAG AGTGTCCGAG AGCGACTTTG AGGACCTGCT GTCCCACCAG 
GGCTTCTCGT CCAAGGCTGA CCGGAAGGGG CCAAGGACCA TGGCTGAGAT GCGGCGGCAG 
GAGCAGGCGA GGGACGCAGA CCCGCTGAAG CTGAAGCTCC TGGAGTGGAC AGAGGGCAAG 
GAGAGGAACA TCCGCGCGCT GCTGTCGACC CTGCACACCG TGCTGTGGGA CGGCGAGAGC 
CGCTGGGCCC CCGTGGGCAT GGCCGACCTG GTGACCCCCG GGCAGGTGAA GAAGCAGTAC 
CGCCGCGCCG TGCTGGTGGT GCACCCGGAC AAGGCCGCAG GGCAGCCCTA CGAGCAGTAT 
GCCAAGATGA TCTTCATGGA GCTGAACGAC GCGTGGGCCG AGTTTGAGAG CCAGGGCTCC 
CGGCCGCTCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_536279.4
CDS	119..4033
Translation	MSLLQSALDFLAGPGSLGGAAGRDQSDFVGQTVELGELRLRVRRVLAEGGFAFVYEAQDLASGREYALKRLLSNEEEKNRAIIQEVCFMKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLTELCKGQLVDFLKKIESRGPLSCDTVLKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKFSIPPNDTRYSVFHDLIRATLKVNPEERLSITELVNQLQEIAAARNVNPKSPITELLEQNGGYGNAVPSRGPPPPGGSSSGYSGGLALAEYDQPYGGLLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRLFLDSKHPGHYAVYNLSPRTYRPSKFHNRVSECGWAARRAPNLRSLYSVCRNMHSWLRQDHKNVCVVHCMDGRAASAVAVCSFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYMCDMVAEEPITPHSKPILVKAVVMTPVPLFSKQRNGCRPFCEVYVGDERVTTTSQEYDKMRDFRIEDGKAVIPLGITVQGDVLIVIYHARSTLGGRLQAKVASMKMFQVQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNVEVEVEPRDRPSREAPPWESTSMRGLNPKILFSSREEQQDILSKFGKPELPRQPGSTAQYDAEAGSLDAEPTESDSPQSSGTDTSHFLHTLDWHEEKDTETSPESHFAKENLSTLAEDAGGSEPSDEEAATPSTESRDAADEDTPGPAARLPERDLIFDGATPATPQEPTRQEDSVDLLGLHAEAGLAPPVQASGAAPSTADLLSRLLGGPDAAPEVPPGDLLGGEAPLLFASPAPSSSAQSPPREGPAADPFDPLLLPSGPNTQPCSQPDLFGEFLHSESPAAVPASFPSTHSAPPPACSTDFLHLGDLPAEPGKMTASTSHPDLLGGWEAWAEAPAPAAAAEGPFFSAGGQPAAPSAPGSWTKSQSLDPFADLGDLSSGLQGSPAGFAPGAFGAKTAPPPTGGSSWQQTGRPPAQGTPWPPQAKPPPKACPQPRPNYAANVSVIGGREERGVRAPSFGQKPRVSESDFEDLLSHQGFSSKADRKGPRTMAEMRRQEQARDADPLKLKLLEWTEGKERNIRALLSTLHTVLWDGESRWAPVGMADLVTPGQVKKQYRRAVLVVHPDKAAGQPYEQYAKMIFMELNDAWAEFESQGSRPLF

Target ORF information:

RefSeq Version	XM_536279.7
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_536279.7

ORF Insert Sequence:

ATGTCGCTGC TGCAGTCTGC GCTGGACTTC CTGGCGGGCC CGGGCTCCCT GGGCGGCGCC 
GCCGGCCGCG ACCAGAGCGA CTTCGTGGGG CAGACGGTGG AGCTGGGCGA GCTGCGGCTG 
CGGGTGCGGC GGGTCCTGGC GGAGGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATCTG 
GCAAGTGGCA GAGAATATGC CTTAAAGAGA TTATTGTCTA ACGAAGAGGA AAAGAACAGA 
GCCATCATTC AGGAAGTTTG TTTCATGAAA AAACTTTCTG GCCACCCCAA TATTGTCCAG 
TTTTGTTCTG CAGCGTCCAT AGGGAAAGAG GAGTCGGACA CTGGGCAGGC TGAGTTCCTG 
CTGCTCACCG AGCTCTGCAA AGGGCAGCTG GTGGACTTTT TAAAGAAAAT TGAATCTAGA 
GGTCCGCTCT CGTGCGATAC TGTTCTGAAG ATTTTCTATC AGACGTGCAG AGCGGTGCAG 
CACATGCACA GACAAAAGCC GCCCATCATC CATAGAGACC TCAAGGTGGA AAACTTATTG 
CTCAGTAACC AAGGGACCAT CAAGCTGTGT GACTTTGGCA GTGCCACGAC CATATCACAT 
TACCCCGACT ACAGCTGGAG CGCCCAGAGG CGGGCCCTGG TGGAGGAAGA GATCACCAGG 
AACACCACAC CCATGTACAG GACACCGGAA ATCGTGGACT TGTACTCCAA CTTCCCCATC 
GGCGAGAAGC AGGACATCTG GGCGCTGGGC TGCATCTTGT ATCTGCTGTG CTTCCGGCAG 
CATCCTTTTG AAGACGGAGC AAAGCTTCGG ATAGTCAACG GGAAGTTCTC GATCCCCCCC 
AATGACACCC GGTACTCTGT GTTCCACGAC CTCATCCGCG CCACGCTGAA GGTCAACCCC 
GAGGAGCGGC TGTCCATCAC AGAACTGGTG AACCAGCTCC AGGAGATCGC GGCCGCCAGG 
AACGTGAACC CCAAGTCTCC CATCACTGAG CTCCTAGAGC AGAACGGAGG CTATGGGAAC 
GCTGTCCCTT CCCGAGGGCC ACCCCCTCCT GGGGGCTCCA GCAGCGGCTA CAGCGGAGGC 
CTGGCGCTGG CCGAGTACGA CCAGCCCTAC GGCGGGCTCC TGGACATCCT GCGGGGGGGC 
ACGGAGCGCC TCTTCACAAA CCTCAAGGAC ACCTCCTCCA AGGTCATCCA GTCTGTTGCC 
AATTACGCAA AAGGAGATTT GGACATCTCT TACATCACAT CTAGAATTGC TGTGATGTCG 
TTCCCAGCAG AAGGCGTGGA GTCGGCCATC AAGAACAACA TCGAAGACGT GCGGTTGTTT 
CTGGACTCCA AGCACCCGGG ACATTACGCC GTCTACAACC TGTCCCCAAG AACATACCGG 
CCCTCCAAAT TTCACAACCG GGTCTCCGAG TGTGGCTGGG CGGCCAGACG GGCCCCGAAC 
CTTCGCAGCC TGTACAGTGT GTGTCGGAAC ATGCACTCGT GGCTCCGGCA GGACCACAAG 
AACGTCTGTG TCGTGCACTG CATGGACGGG AGAGCTGCGT CTGCGGTGGC CGTGTGCTCC 
TTCCTGTGCT TCTGCCGCCT GTTCAGCACC GCGGAGGCTG CCGTGTACAT GTTCAGCATG 
AAGCGCTGCC CGCCAGGCAT TTGGCCATCC CACAAGAGGT ACATCGAGTA CATGTGTGAC 
ATGGTGGCCG AGGAGCCCAT CACACCCCAT AGCAAGCCCA TCCTGGTGAA AGCCGTGGTC 
ATGACACCCG TGCCCCTGTT CAGCAAGCAG AGAAACGGCT GCAGGCCCTT CTGCGAGGTC 
TACGTGGGGG ATGAGCGCGT GACTACCACG TCTCAGGAGT ACGACAAGAT GAGGGATTTC 
AGAATCGAGG ATGGCAAAGC AGTCATTCCC CTGGGCATAA CAGTGCAAGG GGACGTGCTC 
ATTGTCATCT ACCACGCCAG GTCCACCCTG GGAGGAAGAC TGCAGGCCAA GGTGGCATCC 
ATGAAGATGT TCCAGGTCCA GTTCCACACG GGGTTTGTGC CTCGGAATGC GACTACTGTG 
AAGTTTGCAA AGTATGACCT TGATGCGTGT GACATTCAGG AGAAATACCC AGATTTATTC 
CAAGTGAACG TGGAGGTGGA GGTGGAGCCC AGAGACAGGC CCAGCCGGGA GGCCCCACCC 
TGGGAGAGCA CCAGCATGAG GGGGCTCAAC CCCAAAATCC TGTTTTCCAG CCGAGAGGAG 
CAGCAAGACA TTCTGTCTAA ATTTGGGAAG CCGGAGCTCC CCAGGCAGCC CGGCTCCACC 
GCTCAGTACG ACGCCGAGGC AGGGTCCCTG GACGCAGAGC CCACAGAGTC TGATTCACCA 
CAGAGCAGCG GCACTGACAC CAGCCACTTC CTCCACACGC TGGATTGGCA CGAGGAGAAA 
GATACAGAGA CGAGTCCAGA AAGTCACTTT GCCAAGGAGA ACCTATCAAC CCTGGCCGAG 
GATGCAGGTG GGAGTGAGCC TTCGGACGAG GAAGCAGCCA CACCATCCAC CGAGAGCAGG 
GATGCTGCGG ATGAGGACAC ACCAGGGCCA GCGGCGAGGC TGCCAGAGCG AGATTTGATT 
TTTGATGGGG CCACGCCGGC CACACCGCAG GAGCCCACAC GGCAGGAGGA CAGTGTCGAC 
CTCCTAGGGC TGCATGCCGA GGCTGGGCTG GCCCCCCCTG TGCAGGCCAG TGGGGCAGCC 
CCCAGCACCG CCGACCTGCT CAGCCGCCTC CTGGGGGGCC CTGACGCTGC TCCGGAGGTG 
CCCCCGGGTG ACCTGCTGGG TGGAGAGGCT CCCCTCCTCT TCGCAAGCCC AGCCCCCTCC 
TCGAGCGCAC AGAGCCCGCC CCGTGAGGGG CCAGCTGCCG ACCCCTTTGA CCCTCTCCTG 
CTGCCATCTG GGCCCAACAC CCAGCCGTGC TCCCAGCCTG ACCTGTTCGG TGAATTTCTT 
CATTCGGAAT CTCCTGCCGC CGTGCCTGCG TCCTTCCCGT CCACCCACAG CGCCCCACCG 
CCCGCCTGCA GCACCGACTT CCTGCACCTG GGAGATCTAC CAGCAGAGCC TGGCAAGATG 
ACGGCCTCCA CCAGCCACCC GGATCTGCTG GGGGGGTGGG AGGCCTGGGC CGAGGCGCCG 
GCCCCCGCAG CTGCTGCAGA AGGTCCCTTC TTCTCTGCAG GAGGCCAGCC TGCCGCCCCC 
AGTGCCCCCG GGAGCTGGAC CAAGTCTCAG AGCCTGGACC CGTTTGCTGA CCTCGGTGAC 
CTCAGTTCTG GCCTCCAAGG CTCACCGGCT GGATTTGCTC CAGGGGCTTT TGGCGCCAAG 
ACGGCCCCCC CTCCCACGGG CGGCAGCTCC TGGCAGCAGA CGGGTCGGCC CCCGGCCCAG 
GGCACCCCGT GGCCCCCCCA GGCCAAGCCG CCCCCCAAGG CCTGTCCGCA GCCAAGGCCT 
AACTATGCTG CCAACGTGAG CGTGATTGGC GGCCGAGAGG AGAGAGGGGT CCGGGCCCCC 
AGCTTCGGCC AGAAGCCGAG AGTGTCCGAG AGCGACTTTG AGGACCTGCT GTCCCACCAG 
GGCTTCTCGT CCAAGGCTGA CCGGAAGGGG CCAAGGACCA TGGCTGAGAT GCGGCGGCAG 
GAGCAGGCGA GGGACGCAGA CCCGCTGAAG CTGAAGCTCC TGGAGTGGAC AGAGGGCAAG 
GAGAGGAACA TCCGCGCGCT GCTGTCGACC CTGCACACCG TGCTGTGGGA CGGCGAGAGC 
CGCTGGGCCC CCGTGGGCAT GGCCGACCTG GTGACCCCCG GGCAGGTGAA GAAGCAGTAC 
CGCCGCGCCG TGCTGGTGGT GCACCCGGAC AAGGCCGCAG GGCAGCCCTA CGAGCAGTAT 
GCCAAGATGA TCTTCATGGA GCTGAACGAC GCGTGGGCCG AGTTTGAGAG CCAGGGCTCC 
CGGCCGCTCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAK ( XM_536279.6 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_536279.4 Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe04651
Clone ID Related Accession (Same CDS sequence)	XM_536279.7 , XM_536279.6
Accession Version	XM_536279.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	cyclin-G-associated kinase isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_536279.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTGC TGCAGTCTGC GCTGGACTTC CTGGCGGGCC CGGGCTCCCT GGGCGGCGCC 
GCCGGCCGCG ACCAGAGCGA CTTCGTGGGG CAGACGGTGG AGCTGGGCGA GCTGCGGCTG 
CGGGTGCGGC GGGTCCTGGC GGAGGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATCTG 
GCAAGTGGCA GAGAATATGC CTTAAAGAGA TTATTGTCTA ACGAAGAGGA AAAGAACAGA 
GCCATCATTC AGGAAGTTTG TTTCATGAAA AAACTTTCTG GCCACCCCAA TATTGTCCAG 
TTTTGTTCTG CAGCGTCCAT AGGGAAAGAG GAGTCGGACA CTGGGCAGGC TGAGTTCCTG 
CTGCTCACCG AGCTCTGCAA AGGGCAGCTG GTGGACTTTT TAAAGAAAAT TGAATCTAGA 
GGTCCGCTCT CGTGCGATAC TGTTCTGAAG ATTTTCTATC AGACGTGCAG AGCGGTGCAG 
CACATGCACA GACAAAAGCC GCCCATCATC CATAGAGACC TCAAGGTGGA AAACTTATTG 
CTCAGTAACC AAGGGACCAT CAAGCTGTGT GACTTTGGCA GTGCCACGAC CATATCACAT 
TACCCCGACT ACAGCTGGAG CGCCCAGAGG CGGGCCCTGG TGGAGGAAGA GATCACCAGG 
AACACCACAC CCATGTACAG GACACCGGAA ATCGTGGACT TGTACTCCAA CTTCCCCATC 
GGCGAGAAGC AGGACATCTG GGCGCTGGGC TGCATCTTGT ATCTGCTGTG CTTCCGGCAG 
CATCCTTTTG AAGACGGAGC AAAGCTTCGG ATAGTCAACG GGAAGTTCTC GATCCCCCCC 
AATGACACCC GGTACTCTGT GTTCCACGAC CTCATCCGCG CCACGCTGAA GGTCAACCCC 
GAGGAGCGGC TGTCCATCAC AGAACTGGTG AACCAGCTCC AGGAGATCGC GGCCGCCAGG 
AACGTGAACC CCAAGTCTCC CATCACTGAG CTCCTAGAGC AGAACGGAGG CTATGGGAAC 
GCTGTCCCTT CCCGAGGGCC ACCCCCTCCT GGGGGCTCCA GCAGCGGCTA CAGCGGAGGC 
CTGGCGCTGG CCGAGTACGA CCAGCCCTAC GGCGGGCTCC TGGACATCCT GCGGGGGGGC 
ACGGAGCGCC TCTTCACAAA CCTCAAGGAC ACCTCCTCCA AGGTCATCCA GTCTGTTGCC 
AATTACGCAA AAGGAGATTT GGACATCTCT TACATCACAT CTAGAATTGC TGTGATGTCG 
TTCCCAGCAG AAGGCGTGGA GTCGGCCATC AAGAACAACA TCGAAGACGT GCGGTTGTTT 
CTGGACTCCA AGCACCCGGG ACATTACGCC GTCTACAACC TGTCCCCAAG AACATACCGG 
CCCTCCAAAT TTCACAACCG GGTCTCCGAG TGTGGCTGGG CGGCCAGACG GGCCCCGAAC 
CTTCGCAGCC TGTACAGTGT GTGTCGGAAC ATGCACTCGT GGCTCCGGCA GGACCACAAG 
AACGTCTGTG TCGTGCACTG CATGGACGGG AGAGCTGCGT CTGCGGTGGC CGTGTGCTCC 
TTCCTGTGCT TCTGCCGCCT GTTCAGCACC GCGGAGGCTG CCGTGTACAT GTTCAGCATG 
AAGCGCTGCC CGCCAGGCAT TTGGCCATCC CACAAGAGGT ACATCGAGTA CATGTGTGAC 
ATGGTGGCCG AGGAGCCCAT CACACCCCAT AGCAAGCCCA TCCTGGTGAA AGCCGTGGTC 
ATGACACCCG TGCCCCTGTT CAGCAAGCAG AGAAACGGCT GCAGGCCCTT CTGCGAGGTC 
TACGTGGGGG ATGAGCGCGT GACTACCACG TCTCAGGAGT ACGACAAGAT GAGGGATTTC 
AGAATCGAGG ATGGCAAAGC AGTCATTCCC CTGGGCATAA CAGTGCAAGG GGACGTGCTC 
ATTGTCATCT ACCACGCCAG GTCCACCCTG GGAGGAAGAC TGCAGGCCAA GGTGGCATCC 
ATGAAGATGT TCCAGGTCCA GTTCCACACG GGGTTTGTGC CTCGGAATGC GACTACTGTG 
AAGTTTGCAA AGTATGACCT TGATGCGTGT GACATTCAGG AGAAATACCC AGATTTATTC 
CAAGTGAACG TGGAGGTGGA GGTGGAGCCC AGAGACAGGC CCAGCCGGGA GGCCCCACCC 
TGGGAGAGCA CCAGCATGAG GGGGCTCAAC CCCAAAATCC TGTTTTCCAG CCGAGAGGAG 
CAGCAAGACA TTCTGTCTAA ATTTGGGAAG CCGGAGCTCC CCAGGCAGCC CGGCTCCACC 
GCTCAGTACG ACGCCGAGGC AGGGTCCCTG GACGCAGAGC CCACAGAGTC TGATTCACCA 
CAGAGCAGCG GCACTGACAC CAGCCACTTC CTCCACACGC TGGATTGGCA CGAGGAGAAA 
GATACAGAGA CGAGTCCAGA AAGTCACTTT GCCAAGGAGA ACCTATCAAC CCTGGCCGAG 
GATGCAGGTG GGAGTGAGCC TTCGGACGAG GAAGCAGCCA CACCATCCAC CGAGAGCAGG 
GATGCTGCGG ATGAGGACAC ACCAGGGCCA GCGGCGAGGC TGCCAGAGCG AGATTTGATT 
TTTGATGGGG CCACGCCGGC CACACCGCAG GAGCCCACAC GGCAGGAGGA CAGTGTCGAC 
CTCCTAGGGC TGCATGCCGA GGCTGGGCTG GCCCCCCCTG TGCAGGCCAG TGGGGCAGCC 
CCCAGCACCG CCGACCTGCT CAGCCGCCTC CTGGGGGGCC CTGACGCTGC TCCGGAGGTG 
CCCCCGGGTG ACCTGCTGGG TGGAGAGGCT CCCCTCCTCT TCGCAAGCCC AGCCCCCTCC 
TCGAGCGCAC AGAGCCCGCC CCGTGAGGGG CCAGCTGCCG ACCCCTTTGA CCCTCTCCTG 
CTGCCATCTG GGCCCAACAC CCAGCCGTGC TCCCAGCCTG ACCTGTTCGG TGAATTTCTT 
CATTCGGAAT CTCCTGCCGC CGTGCCTGCG TCCTTCCCGT CCACCCACAG CGCCCCACCG 
CCCGCCTGCA GCACCGACTT CCTGCACCTG GGAGATCTAC CAGCAGAGCC TGGCAAGATG 
ACGGCCTCCA CCAGCCACCC GGATCTGCTG GGGGGGTGGG AGGCCTGGGC CGAGGCGCCG 
GCCCCCGCAG CTGCTGCAGA AGGTCCCTTC TTCTCTGCAG GAGGCCAGCC TGCCGCCCCC 
AGTGCCCCCG GGAGCTGGAC CAAGTCTCAG AGCCTGGACC CGTTTGCTGA CCTCGGTGAC 
CTCAGTTCTG GCCTCCAAGG CTCACCGGCT GGATTTGCTC CAGGGGCTTT TGGCGCCAAG 
ACGGCCCCCC CTCCCACGGG CGGCAGCTCC TGGCAGCAGA CGGGTCGGCC CCCGGCCCAG 
GGCACCCCGT GGCCCCCCCA GGCCAAGCCG CCCCCCAAGG CCTGTCCGCA GCCAAGGCCT 
AACTATGCTG CCAACGTGAG CGTGATTGGC GGCCGAGAGG AGAGAGGGGT CCGGGCCCCC 
AGCTTCGGCC AGAAGCCGAG AGTGTCCGAG AGCGACTTTG AGGACCTGCT GTCCCACCAG 
GGCTTCTCGT CCAAGGCTGA CCGGAAGGGG CCAAGGACCA TGGCTGAGAT GCGGCGGCAG 
GAGCAGGCGA GGGACGCAGA CCCGCTGAAG CTGAAGCTCC TGGAGTGGAC AGAGGGCAAG 
GAGAGGAACA TCCGCGCGCT GCTGTCGACC CTGCACACCG TGCTGTGGGA CGGCGAGAGC 
CGCTGGGCCC CCGTGGGCAT GGCCGACCTG GTGACCCCCG GGCAGGTGAA GAAGCAGTAC 
CGCCGCGCCG TGCTGGTGGT GCACCCGGAC AAGGCCGCAG GGCAGCCCTA CGAGCAGTAT 
GCCAAGATGA TCTTCATGGA GCTGAACGAC GCGTGGGCCG AGTTTGAGAG CCAGGGCTCC 
CGGCCGCTCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_536279.4
CDS	164..4078
Translation	MSLLQSALDFLAGPGSLGGAAGRDQSDFVGQTVELGELRLRVRRVLAEGGFAFVYEAQDLASGREYALKRLLSNEEEKNRAIIQEVCFMKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLTELCKGQLVDFLKKIESRGPLSCDTVLKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKFSIPPNDTRYSVFHDLIRATLKVNPEERLSITELVNQLQEIAAARNVNPKSPITELLEQNGGYGNAVPSRGPPPPGGSSSGYSGGLALAEYDQPYGGLLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRLFLDSKHPGHYAVYNLSPRTYRPSKFHNRVSECGWAARRAPNLRSLYSVCRNMHSWLRQDHKNVCVVHCMDGRAASAVAVCSFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYMCDMVAEEPITPHSKPILVKAVVMTPVPLFSKQRNGCRPFCEVYVGDERVTTTSQEYDKMRDFRIEDGKAVIPLGITVQGDVLIVIYHARSTLGGRLQAKVASMKMFQVQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNVEVEVEPRDRPSREAPPWESTSMRGLNPKILFSSREEQQDILSKFGKPELPRQPGSTAQYDAEAGSLDAEPTESDSPQSSGTDTSHFLHTLDWHEEKDTETSPESHFAKENLSTLAEDAGGSEPSDEEAATPSTESRDAADEDTPGPAARLPERDLIFDGATPATPQEPTRQEDSVDLLGLHAEAGLAPPVQASGAAPSTADLLSRLLGGPDAAPEVPPGDLLGGEAPLLFASPAPSSSAQSPPREGPAADPFDPLLLPSGPNTQPCSQPDLFGEFLHSESPAAVPASFPSTHSAPPPACSTDFLHLGDLPAEPGKMTASTSHPDLLGGWEAWAEAPAPAAAAEGPFFSAGGQPAAPSAPGSWTKSQSLDPFADLGDLSSGLQGSPAGFAPGAFGAKTAPPPTGGSSWQQTGRPPAQGTPWPPQAKPPPKACPQPRPNYAANVSVIGGREERGVRAPSFGQKPRVSESDFEDLLSHQGFSSKADRKGPRTMAEMRRQEQARDADPLKLKLLEWTEGKERNIRALLSTLHTVLWDGESRWAPVGMADLVTPGQVKKQYRRAVLVVHPDKAAGQPYEQYAKMIFMELNDAWAEFESQGSRPLF

Target ORF information:

RefSeq Version	XM_536279.6
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_536279.6

ORF Insert Sequence:

ATGTCGCTGC TGCAGTCTGC GCTGGACTTC CTGGCGGGCC CGGGCTCCCT GGGCGGCGCC 
GCCGGCCGCG ACCAGAGCGA CTTCGTGGGG CAGACGGTGG AGCTGGGCGA GCTGCGGCTG 
CGGGTGCGGC GGGTCCTGGC GGAGGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATCTG 
GCAAGTGGCA GAGAATATGC CTTAAAGAGA TTATTGTCTA ACGAAGAGGA AAAGAACAGA 
GCCATCATTC AGGAAGTTTG TTTCATGAAA AAACTTTCTG GCCACCCCAA TATTGTCCAG 
TTTTGTTCTG CAGCGTCCAT AGGGAAAGAG GAGTCGGACA CTGGGCAGGC TGAGTTCCTG 
CTGCTCACCG AGCTCTGCAA AGGGCAGCTG GTGGACTTTT TAAAGAAAAT TGAATCTAGA 
GGTCCGCTCT CGTGCGATAC TGTTCTGAAG ATTTTCTATC AGACGTGCAG AGCGGTGCAG 
CACATGCACA GACAAAAGCC GCCCATCATC CATAGAGACC TCAAGGTGGA AAACTTATTG 
CTCAGTAACC AAGGGACCAT CAAGCTGTGT GACTTTGGCA GTGCCACGAC CATATCACAT 
TACCCCGACT ACAGCTGGAG CGCCCAGAGG CGGGCCCTGG TGGAGGAAGA GATCACCAGG 
AACACCACAC CCATGTACAG GACACCGGAA ATCGTGGACT TGTACTCCAA CTTCCCCATC 
GGCGAGAAGC AGGACATCTG GGCGCTGGGC TGCATCTTGT ATCTGCTGTG CTTCCGGCAG 
CATCCTTTTG AAGACGGAGC AAAGCTTCGG ATAGTCAACG GGAAGTTCTC GATCCCCCCC 
AATGACACCC GGTACTCTGT GTTCCACGAC CTCATCCGCG CCACGCTGAA GGTCAACCCC 
GAGGAGCGGC TGTCCATCAC AGAACTGGTG AACCAGCTCC AGGAGATCGC GGCCGCCAGG 
AACGTGAACC CCAAGTCTCC CATCACTGAG CTCCTAGAGC AGAACGGAGG CTATGGGAAC 
GCTGTCCCTT CCCGAGGGCC ACCCCCTCCT GGGGGCTCCA GCAGCGGCTA CAGCGGAGGC 
CTGGCGCTGG CCGAGTACGA CCAGCCCTAC GGCGGGCTCC TGGACATCCT GCGGGGGGGC 
ACGGAGCGCC TCTTCACAAA CCTCAAGGAC ACCTCCTCCA AGGTCATCCA GTCTGTTGCC 
AATTACGCAA AAGGAGATTT GGACATCTCT TACATCACAT CTAGAATTGC TGTGATGTCG 
TTCCCAGCAG AAGGCGTGGA GTCGGCCATC AAGAACAACA TCGAAGACGT GCGGTTGTTT 
CTGGACTCCA AGCACCCGGG ACATTACGCC GTCTACAACC TGTCCCCAAG AACATACCGG 
CCCTCCAAAT TTCACAACCG GGTCTCCGAG TGTGGCTGGG CGGCCAGACG GGCCCCGAAC 
CTTCGCAGCC TGTACAGTGT GTGTCGGAAC ATGCACTCGT GGCTCCGGCA GGACCACAAG 
AACGTCTGTG TCGTGCACTG CATGGACGGG AGAGCTGCGT CTGCGGTGGC CGTGTGCTCC 
TTCCTGTGCT TCTGCCGCCT GTTCAGCACC GCGGAGGCTG CCGTGTACAT GTTCAGCATG 
AAGCGCTGCC CGCCAGGCAT TTGGCCATCC CACAAGAGGT ACATCGAGTA CATGTGTGAC 
ATGGTGGCCG AGGAGCCCAT CACACCCCAT AGCAAGCCCA TCCTGGTGAA AGCCGTGGTC 
ATGACACCCG TGCCCCTGTT CAGCAAGCAG AGAAACGGCT GCAGGCCCTT CTGCGAGGTC 
TACGTGGGGG ATGAGCGCGT GACTACCACG TCTCAGGAGT ACGACAAGAT GAGGGATTTC 
AGAATCGAGG ATGGCAAAGC AGTCATTCCC CTGGGCATAA CAGTGCAAGG GGACGTGCTC 
ATTGTCATCT ACCACGCCAG GTCCACCCTG GGAGGAAGAC TGCAGGCCAA GGTGGCATCC 
ATGAAGATGT TCCAGGTCCA GTTCCACACG GGGTTTGTGC CTCGGAATGC GACTACTGTG 
AAGTTTGCAA AGTATGACCT TGATGCGTGT GACATTCAGG AGAAATACCC AGATTTATTC 
CAAGTGAACG TGGAGGTGGA GGTGGAGCCC AGAGACAGGC CCAGCCGGGA GGCCCCACCC 
TGGGAGAGCA CCAGCATGAG GGGGCTCAAC CCCAAAATCC TGTTTTCCAG CCGAGAGGAG 
CAGCAAGACA TTCTGTCTAA ATTTGGGAAG CCGGAGCTCC CCAGGCAGCC CGGCTCCACC 
GCTCAGTACG ACGCCGAGGC AGGGTCCCTG GACGCAGAGC CCACAGAGTC TGATTCACCA 
CAGAGCAGCG GCACTGACAC CAGCCACTTC CTCCACACGC TGGATTGGCA CGAGGAGAAA 
GATACAGAGA CGAGTCCAGA AAGTCACTTT GCCAAGGAGA ACCTATCAAC CCTGGCCGAG 
GATGCAGGTG GGAGTGAGCC TTCGGACGAG GAAGCAGCCA CACCATCCAC CGAGAGCAGG 
GATGCTGCGG ATGAGGACAC ACCAGGGCCA GCGGCGAGGC TGCCAGAGCG AGATTTGATT 
TTTGATGGGG CCACGCCGGC CACACCGCAG GAGCCCACAC GGCAGGAGGA CAGTGTCGAC 
CTCCTAGGGC TGCATGCCGA GGCTGGGCTG GCCCCCCCTG TGCAGGCCAG TGGGGCAGCC 
CCCAGCACCG CCGACCTGCT CAGCCGCCTC CTGGGGGGCC CTGACGCTGC TCCGGAGGTG 
CCCCCGGGTG ACCTGCTGGG TGGAGAGGCT CCCCTCCTCT TCGCAAGCCC AGCCCCCTCC 
TCGAGCGCAC AGAGCCCGCC CCGTGAGGGG CCAGCTGCCG ACCCCTTTGA CCCTCTCCTG 
CTGCCATCTG GGCCCAACAC CCAGCCGTGC TCCCAGCCTG ACCTGTTCGG TGAATTTCTT 
CATTCGGAAT CTCCTGCCGC CGTGCCTGCG TCCTTCCCGT CCACCCACAG CGCCCCACCG 
CCCGCCTGCA GCACCGACTT CCTGCACCTG GGAGATCTAC CAGCAGAGCC TGGCAAGATG 
ACGGCCTCCA CCAGCCACCC GGATCTGCTG GGGGGGTGGG AGGCCTGGGC CGAGGCGCCG 
GCCCCCGCAG CTGCTGCAGA AGGTCCCTTC TTCTCTGCAG GAGGCCAGCC TGCCGCCCCC 
AGTGCCCCCG GGAGCTGGAC CAAGTCTCAG AGCCTGGACC CGTTTGCTGA CCTCGGTGAC 
CTCAGTTCTG GCCTCCAAGG CTCACCGGCT GGATTTGCTC CAGGGGCTTT TGGCGCCAAG 
ACGGCCCCCC CTCCCACGGG CGGCAGCTCC TGGCAGCAGA CGGGTCGGCC CCCGGCCCAG 
GGCACCCCGT GGCCCCCCCA GGCCAAGCCG CCCCCCAAGG CCTGTCCGCA GCCAAGGCCT 
AACTATGCTG CCAACGTGAG CGTGATTGGC GGCCGAGAGG AGAGAGGGGT CCGGGCCCCC 
AGCTTCGGCC AGAAGCCGAG AGTGTCCGAG AGCGACTTTG AGGACCTGCT GTCCCACCAG 
GGCTTCTCGT CCAAGGCTGA CCGGAAGGGG CCAAGGACCA TGGCTGAGAT GCGGCGGCAG 
GAGCAGGCGA GGGACGCAGA CCCGCTGAAG CTGAAGCTCC TGGAGTGGAC AGAGGGCAAG 
GAGAGGAACA TCCGCGCGCT GCTGTCGACC CTGCACACCG TGCTGTGGGA CGGCGAGAGC 
CGCTGGGCCC CCGTGGGCAT GGCCGACCTG GTGACCCCCG GGCAGGTGAA GAAGCAGTAC 
CGCCGCGCCG TGCTGGTGGT GCACCCGGAC AAGGCCGCAG GGCAGCCCTA CGAGCAGTAT 
GCCAAGATGA TCTTCATGGA GCTGAACGAC GCGTGGGCCG AGTTTGAGAG CCAGGGCTCC 
CGGCCGCTCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAK ( XM_005618686.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005618743.1 Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X3, mRNA.

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CloneID	OCe04652
Clone ID Related Accession (Same CDS sequence)	XM_005618686.2 , XM_022417287.1
Accession Version	XM_005618686.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	cyclin-G-associated kinase isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005618686.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCTG TCTGCTGTCC TGCCTGGCCT GTGGTCAGAC TTACGCATTC TCCTTTAACA 
AGACACCTGC AGAAAAAACT TTCTGGCCAC CCCAATATTG TCCAGTTTTG TTCTGCAGCG 
TCCATAGGGA AAGAGGAGTC GGACACTGGG CAGGCTGAGT TCCTGCTGCT CACCGAGCTC 
TGCAAAGGGC AGCTGGTGGA CTTTTTAAAG AAAATTGAAT CTAGAGGTCC GCTCTCGTGC 
GATACTGTTC TGAAGATTTT CTATCAGACG TGCAGAGCGG TGCAGCACAT GCACAGACAA 
AAGCCGCCCA TCATCCATAG AGACCTCAAG GTGGAAAACT TATTGCTCAG TAACCAAGGG 
ACCATCAAGC TGTGTGACTT TGGCAGTGCC ACGACCATAT CACATTACCC CGACTACAGC 
TGGAGCGCCC AGAGGCGGGC CCTGGTGGAG GAAGAGATCA CCAGGAACAC CACACCCATG 
TACAGGACAC CGGAAATCGT GGACTTGTAC TCCAACTTCC CCATCGGCGA GAAGCAGGAC 
ATCTGGGCGC TGGGCTGCAT CTTGTATCTG CTGTGCTTCC GGCAGCATCC TTTTGAAGAC 
GGAGCAAAGC TTCGGATAGT CAACGGGAAG TTCTCGATCC CCCCCAATGA CACCCGGTAC 
TCTGTGTTCC ACGACCTCAT CCGCGCCACG CTGAAGGTCA ACCCCGAGGA GCGGCTGTCC 
ATCACAGAAC TGGTGAACCA GCTCCAGGAG ATCGCGGCCG CCAGGAACGT GAACCCCAAG 
TCTCCCATCA CTGAGCTCCT AGAGCAGAAC GGAGGCTATG GGAACGCTGT CCCTTCCCGA 
GGGCCACCCC CTCCTGGGGG CTCCAGCAGC GGCTACAGCG GAGGCCTGGC GCTGGCCGAG 
TACGACCAGC CCTACGGCGG GCTCCTGGAC ATCCTGCGGG GGGGCACGGA GCGCCTCTTC 
ACAAACCTCA AGGACACCTC CTCCAAGGTC ATCCAGTCTG TTGCCAATTA CGCAAAAGGA 
GATTTGGACA TCTCTTACAT CACATCTAGA ATTGCTGTGA TGTCGTTCCC AGCAGAAGGC 
GTGGAGTCGG CCATCAAGAA CAACATCGAA GACGTGCGGT TGTTTCTGGA CTCCAAGCAC 
CCGGGACATT ACGCCGTCTA CAACCTGTCC CCAAGAACAT ACCGGCCCTC CAAATTTCAC 
AACCGGGTCT CCGAGTGTGG CTGGGCGGCC AGACGGGCCC CGAACCTTCG CAGCCTGTAC 
AGTGTGTGTC GGAACATGCA CTCGTGGCTC CGGCAGGACC ACAAGAACGT CTGTGTCGTG 
CACTGCATGG ACGGGAGAGC TGCGTCTGCG GTGGCCGTGT GCTCCTTCCT GTGCTTCTGC 
CGCCTGTTCA GCACCGCGGA GGCTGCCGTG TACATGTTCA GCATGAAGCG CTGCCCGCCA 
GGCATTTGGC CATCCCACAA GAGGTACATC GAGTACATGT GTGACATGGT GGCCGAGGAG 
CCCATCACAC CCCATAGCAA GCCCATCCTG GTGAAAGCCG TGGTCATGAC ACCCGTGCCC 
CTGTTCAGCA AGCAGAGAAA CGGCTGCAGG CCCTTCTGCG AGGTCTACGT GGGGGATGAG 
CGCGTGACTA CCACGTCTCA GGAGTACGAC AAGATGAGGG ATTTCAGAAT CGAGGATGGC 
AAAGCAGTCA TTCCCCTGGG CATAACAGTG CAAGGGGACG TGCTCATTGT CATCTACCAC 
GCCAGGTCCA CCCTGGGAGG AAGACTGCAG GCCAAGGTGG CATCCATGAA GATGTTCCAG 
GTCCAGTTCC ACACGGGGTT TGTGCCTCGG AATGCGACTA CTGTGAAGTT TGCAAAGTAT 
GACCTTGATG CGTGTGACAT TCAGGAGAAA TACCCAGATT TATTCCAAGT GAACGTGGAG 
GTGGAGGTGG AGCCCAGAGA CAGGCCCAGC CGGGAGGCCC CACCCTGGGA GAGCACCAGC 
ATGAGGGGGC TCAACCCCAA AATCCTGTTT TCCAGCCGAG AGGAGCAGCA AGACATTCTG 
TCTAAATTTG GGAAGCCGGA GCTCCCCAGG CAGCCCGGCT CCACCGCTCA GTACGACGCC 
GAGGCAGGGT CCCTGGACGC AGAGCCCACA GAGTCTGATT CACCACAGAG CAGCGGCACT 
GACACCAGCC ACTTCCTCCA CACGCTGGAT TGGCACGAGG AGAAAGATAC AGAGACGAGT 
CCAGAAAGTC ACTTTGCCAA GGAGAACCTA TCAACCCTGG CCGAGGATGC AGGTGGGAGT 
GAGCCTTCGG ACGAGGAAGC AGCCACACCA TCCACCGAGA GCAGGGATGC TGCGGATGAG 
GACACACCAG GGCCAGCGGC GAGGCTGCCA GAGCGAGATT TGATTTTTGA TGGGGCCACG 
CCGGCCACAC CGCAGGAGCC CACACGGCAG GAGGACAGTG TCGACCTCCT AGGGCTGCAT 
GCCGAGGCTG GGCTGGCCCC CCCTGTGCAG GCCAGTGGGG CAGCCCCCAG CACCGCCGAC 
CTGCTCAGCC GCCTCCTGGG GGGCCCTGAC GCTGCTCCGG AGGTGCCCCC GGGTGACCTG 
CTGGGTGGAG AGGCTCCCCT CCTCTTCGCA AGCCCAGCCC CCTCCTCGAG CGCACAGAGC 
CCGCCCCGTG AGGGGCCAGC TGCCGACCCC TTTGACCCTC TCCTGCTGCC ATCTGGGCCC 
AACACCCAGC CGTGCTCCCA GCCTGACCTG TTCGGTGAAT TTCTTCATTC GGAATCTCCT 
GCCGCCGTGC CTGCGTCCTT CCCGTCCACC CACAGCGCCC CACCGCCCGC CTGCAGCACC 
GACTTCCTGC ACCTGGGAGA TCTACCAGCA GAGCCTGGCA AGATGACGGC CTCCACCAGC 
CACCCGGATC TGCTGGGGGG GTGGGAGGCC TGGGCCGAGG CGCCGGCCCC CGCAGCTGCT 
GCAGAAGGTC CCTTCTTCTC TGCAGGAGGC CAGCCTGCCG CCCCCAGTGC CCCCGGGAGC 
TGGACCAAGT CTCAGAGCCT GGACCCGTTT GCTGACCTCG GTGACCTCAG TTCTGGCCTC 
CAAGGCTCAC CGGCTGGATT TGCTCCAGGG GCTTTTGGCG CCAAGACGGC CCCCCCTCCC 
ACGGGCGGCA GCTCCTGGCA GCAGACGGGT CGGCCCCCGG CCCAGGGCAC CCCGTGGCCC 
CCCCAGGCCA AGCCGCCCCC CAAGGCCTGT CCGCAGCCAA GGCCTAACTA TGCTGCCAAC 
GTGAGCGTGA TTGGCGGCCG AGAGGAGAGA GGGGTCCGGG CCCCCAGCTT CGGCCAGAAG 
CCGAGAGTGT CCGAGAGCGA CTTTGAGGAC CTGCTGTCCC ACCAGGGCTT CTCGTCCAAG 
GCTGACCGGA AGGGGCCAAG GACCATGGCT GAGATGCGGC GGCAGGAGCA GGCGAGGGAC 
GCAGACCCGC TGAAGCTGAA GCTCCTGGAG TGGACAGAGG GCAAGGAGAG GAACATCCGC 
GCGCTGCTGT CGACCCTGCA CACCGTGCTG TGGGACGGCG AGAGCCGCTG GGCCCCCGTG 
GGCATGGCCG ACCTGGTGAC CCCCGGGCAG GTGAAGAAGC AGTACCGCCG CGCCGTGCTG 
GTGGTGCACC CGGACAAGGC CGCAGGGCAG CCCTACGAGC AGTATGCCAA GATGATCTTC 
ATGGAGCTGA ACGACGCGTG GGCCGAGTTT GAGAGCCAGG GCTCCCGGCC GCTCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005618743.1
CDS	241..3960
Translation	MPSVCCPAWPVVRLTHSPLTRHLQKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLTELCKGQLVDFLKKIESRGPLSCDTVLKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKFSIPPNDTRYSVFHDLIRATLKVNPEERLSITELVNQLQEIAAARNVNPKSPITELLEQNGGYGNAVPSRGPPPPGGSSSGYSGGLALAEYDQPYGGLLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRLFLDSKHPGHYAVYNLSPRTYRPSKFHNRVSECGWAARRAPNLRSLYSVCRNMHSWLRQDHKNVCVVHCMDGRAASAVAVCSFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYMCDMVAEEPITPHSKPILVKAVVMTPVPLFSKQRNGCRPFCEVYVGDERVTTTSQEYDKMRDFRIEDGKAVIPLGITVQGDVLIVIYHARSTLGGRLQAKVASMKMFQVQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNVEVEVEPRDRPSREAPPWESTSMRGLNPKILFSSREEQQDILSKFGKPELPRQPGSTAQYDAEAGSLDAEPTESDSPQSSGTDTSHFLHTLDWHEEKDTETSPESHFAKENLSTLAEDAGGSEPSDEEAATPSTESRDAADEDTPGPAARLPERDLIFDGATPATPQEPTRQEDSVDLLGLHAEAGLAPPVQASGAAPSTADLLSRLLGGPDAAPEVPPGDLLGGEAPLLFASPAPSSSAQSPPREGPAADPFDPLLLPSGPNTQPCSQPDLFGEFLHSESPAAVPASFPSTHSAPPPACSTDFLHLGDLPAEPGKMTASTSHPDLLGGWEAWAEAPAPAAAAEGPFFSAGGQPAAPSAPGSWTKSQSLDPFADLGDLSSGLQGSPAGFAPGAFGAKTAPPPTGGSSWQQTGRPPAQGTPWPPQAKPPPKACPQPRPNYAANVSVIGGREERGVRAPSFGQKPRVSESDFEDLLSHQGFSSKADRKGPRTMAEMRRQEQARDADPLKLKLLEWTEGKERNIRALLSTLHTVLWDGESRWAPVGMADLVTPGQVKKQYRRAVLVVHPDKAAGQPYEQYAKMIFMELNDAWAEFESQGSRPLF

Target ORF information:

RefSeq Version	XM_005618686.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005618686.2

ORF Insert Sequence:

ATGCCCTCTG TCTGCTGTCC TGCCTGGCCT GTGGTCAGAC TTACGCATTC TCCTTTAACA 
AGACACCTGC AGAAAAAACT TTCTGGCCAC CCCAATATTG TCCAGTTTTG TTCTGCAGCG 
TCCATAGGGA AAGAGGAGTC GGACACTGGG CAGGCTGAGT TCCTGCTGCT CACCGAGCTC 
TGCAAAGGGC AGCTGGTGGA CTTTTTAAAG AAAATTGAAT CTAGAGGTCC GCTCTCGTGC 
GATACTGTTC TGAAGATTTT CTATCAGACG TGCAGAGCGG TGCAGCACAT GCACAGACAA 
AAGCCGCCCA TCATCCATAG AGACCTCAAG GTGGAAAACT TATTGCTCAG TAACCAAGGG 
ACCATCAAGC TGTGTGACTT TGGCAGTGCC ACGACCATAT CACATTACCC CGACTACAGC 
TGGAGCGCCC AGAGGCGGGC CCTGGTGGAG GAAGAGATCA CCAGGAACAC CACACCCATG 
TACAGGACAC CGGAAATCGT GGACTTGTAC TCCAACTTCC CCATCGGCGA GAAGCAGGAC 
ATCTGGGCGC TGGGCTGCAT CTTGTATCTG CTGTGCTTCC GGCAGCATCC TTTTGAAGAC 
GGAGCAAAGC TTCGGATAGT CAACGGGAAG TTCTCGATCC CCCCCAATGA CACCCGGTAC 
TCTGTGTTCC ACGACCTCAT CCGCGCCACG CTGAAGGTCA ACCCCGAGGA GCGGCTGTCC 
ATCACAGAAC TGGTGAACCA GCTCCAGGAG ATCGCGGCCG CCAGGAACGT GAACCCCAAG 
TCTCCCATCA CTGAGCTCCT AGAGCAGAAC GGAGGCTATG GGAACGCTGT CCCTTCCCGA 
GGGCCACCCC CTCCTGGGGG CTCCAGCAGC GGCTACAGCG GAGGCCTGGC GCTGGCCGAG 
TACGACCAGC CCTACGGCGG GCTCCTGGAC ATCCTGCGGG GGGGCACGGA GCGCCTCTTC 
ACAAACCTCA AGGACACCTC CTCCAAGGTC ATCCAGTCTG TTGCCAATTA CGCAAAAGGA 
GATTTGGACA TCTCTTACAT CACATCTAGA ATTGCTGTGA TGTCGTTCCC AGCAGAAGGC 
GTGGAGTCGG CCATCAAGAA CAACATCGAA GACGTGCGGT TGTTTCTGGA CTCCAAGCAC 
CCGGGACATT ACGCCGTCTA CAACCTGTCC CCAAGAACAT ACCGGCCCTC CAAATTTCAC 
AACCGGGTCT CCGAGTGTGG CTGGGCGGCC AGACGGGCCC CGAACCTTCG CAGCCTGTAC 
AGTGTGTGTC GGAACATGCA CTCGTGGCTC CGGCAGGACC ACAAGAACGT CTGTGTCGTG 
CACTGCATGG ACGGGAGAGC TGCGTCTGCG GTGGCCGTGT GCTCCTTCCT GTGCTTCTGC 
CGCCTGTTCA GCACCGCGGA GGCTGCCGTG TACATGTTCA GCATGAAGCG CTGCCCGCCA 
GGCATTTGGC CATCCCACAA GAGGTACATC GAGTACATGT GTGACATGGT GGCCGAGGAG 
CCCATCACAC CCCATAGCAA GCCCATCCTG GTGAAAGCCG TGGTCATGAC ACCCGTGCCC 
CTGTTCAGCA AGCAGAGAAA CGGCTGCAGG CCCTTCTGCG AGGTCTACGT GGGGGATGAG 
CGCGTGACTA CCACGTCTCA GGAGTACGAC AAGATGAGGG ATTTCAGAAT CGAGGATGGC 
AAAGCAGTCA TTCCCCTGGG CATAACAGTG CAAGGGGACG TGCTCATTGT CATCTACCAC 
GCCAGGTCCA CCCTGGGAGG AAGACTGCAG GCCAAGGTGG CATCCATGAA GATGTTCCAG 
GTCCAGTTCC ACACGGGGTT TGTGCCTCGG AATGCGACTA CTGTGAAGTT TGCAAAGTAT 
GACCTTGATG CGTGTGACAT TCAGGAGAAA TACCCAGATT TATTCCAAGT GAACGTGGAG 
GTGGAGGTGG AGCCCAGAGA CAGGCCCAGC CGGGAGGCCC CACCCTGGGA GAGCACCAGC 
ATGAGGGGGC TCAACCCCAA AATCCTGTTT TCCAGCCGAG AGGAGCAGCA AGACATTCTG 
TCTAAATTTG GGAAGCCGGA GCTCCCCAGG CAGCCCGGCT CCACCGCTCA GTACGACGCC 
GAGGCAGGGT CCCTGGACGC AGAGCCCACA GAGTCTGATT CACCACAGAG CAGCGGCACT 
GACACCAGCC ACTTCCTCCA CACGCTGGAT TGGCACGAGG AGAAAGATAC AGAGACGAGT 
CCAGAAAGTC ACTTTGCCAA GGAGAACCTA TCAACCCTGG CCGAGGATGC AGGTGGGAGT 
GAGCCTTCGG ACGAGGAAGC AGCCACACCA TCCACCGAGA GCAGGGATGC TGCGGATGAG 
GACACACCAG GGCCAGCGGC GAGGCTGCCA GAGCGAGATT TGATTTTTGA TGGGGCCACG 
CCGGCCACAC CGCAGGAGCC CACACGGCAG GAGGACAGTG TCGACCTCCT AGGGCTGCAT 
GCCGAGGCTG GGCTGGCCCC CCCTGTGCAG GCCAGTGGGG CAGCCCCCAG CACCGCCGAC 
CTGCTCAGCC GCCTCCTGGG GGGCCCTGAC GCTGCTCCGG AGGTGCCCCC GGGTGACCTG 
CTGGGTGGAG AGGCTCCCCT CCTCTTCGCA AGCCCAGCCC CCTCCTCGAG CGCACAGAGC 
CCGCCCCGTG AGGGGCCAGC TGCCGACCCC TTTGACCCTC TCCTGCTGCC ATCTGGGCCC 
AACACCCAGC CGTGCTCCCA GCCTGACCTG TTCGGTGAAT TTCTTCATTC GGAATCTCCT 
GCCGCCGTGC CTGCGTCCTT CCCGTCCACC CACAGCGCCC CACCGCCCGC CTGCAGCACC 
GACTTCCTGC ACCTGGGAGA TCTACCAGCA GAGCCTGGCA AGATGACGGC CTCCACCAGC 
CACCCGGATC TGCTGGGGGG GTGGGAGGCC TGGGCCGAGG CGCCGGCCCC CGCAGCTGCT 
GCAGAAGGTC CCTTCTTCTC TGCAGGAGGC CAGCCTGCCG CCCCCAGTGC CCCCGGGAGC 
TGGACCAAGT CTCAGAGCCT GGACCCGTTT GCTGACCTCG GTGACCTCAG TTCTGGCCTC 
CAAGGCTCAC CGGCTGGATT TGCTCCAGGG GCTTTTGGCG CCAAGACGGC CCCCCCTCCC 
ACGGGCGGCA GCTCCTGGCA GCAGACGGGT CGGCCCCCGG CCCAGGGCAC CCCGTGGCCC 
CCCCAGGCCA AGCCGCCCCC CAAGGCCTGT CCGCAGCCAA GGCCTAACTA TGCTGCCAAC 
GTGAGCGTGA TTGGCGGCCG AGAGGAGAGA GGGGTCCGGG CCCCCAGCTT CGGCCAGAAG 
CCGAGAGTGT CCGAGAGCGA CTTTGAGGAC CTGCTGTCCC ACCAGGGCTT CTCGTCCAAG 
GCTGACCGGA AGGGGCCAAG GACCATGGCT GAGATGCGGC GGCAGGAGCA GGCGAGGGAC 
GCAGACCCGC TGAAGCTGAA GCTCCTGGAG TGGACAGAGG GCAAGGAGAG GAACATCCGC 
GCGCTGCTGT CGACCCTGCA CACCGTGCTG TGGGACGGCG AGAGCCGCTG GGCCCCCGTG 
GGCATGGCCG ACCTGGTGAC CCCCGGGCAG GTGAAGAAGC AGTACCGCCG CGCCGTGCTG 
GTGGTGCACC CGGACAAGGC CGCAGGGCAG CCCTACGAGC AGTATGCCAA GATGATCTTC 
ATGGAGCTGA ACGACGCGTG GGCCGAGTTT GAGAGCCAGG GCTCCCGGCC GCTCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAK ( XM_022417287.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022272995.1 Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe04652
Clone ID Related Accession (Same CDS sequence)	XM_005618686.2 , XM_022417287.1
Accession Version	XM_022417287.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	cyclin-G-associated kinase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCTG TCTGCTGTCC TGCCTGGCCT GTGGTCAGAC TTACGCATTC TCCTTTAACA 
AGACACCTGC AGAAAAAACT TTCTGGCCAC CCCAATATTG TCCAGTTTTG TTCTGCAGCG 
TCCATAGGGA AAGAGGAGTC GGACACTGGG CAGGCTGAGT TCCTGCTGCT CACCGAGCTC 
TGCAAAGGGC AGCTGGTGGA CTTTTTAAAG AAAATTGAAT CTAGAGGTCC GCTCTCGTGC 
GATACTGTTC TGAAGATTTT CTATCAGACG TGCAGAGCGG TGCAGCACAT GCACAGACAA 
AAGCCGCCCA TCATCCATAG AGACCTCAAG GTGGAAAACT TATTGCTCAG TAACCAAGGG 
ACCATCAAGC TGTGTGACTT TGGCAGTGCC ACGACCATAT CACATTACCC CGACTACAGC 
TGGAGCGCCC AGAGGCGGGC CCTGGTGGAG GAAGAGATCA CCAGGAACAC CACACCCATG 
TACAGGACAC CGGAAATCGT GGACTTGTAC TCCAACTTCC CCATCGGCGA GAAGCAGGAC 
ATCTGGGCGC TGGGCTGCAT CTTGTATCTG CTGTGCTTCC GGCAGCATCC TTTTGAAGAC 
GGAGCAAAGC TTCGGATAGT CAACGGGAAG TTCTCGATCC CCCCCAATGA CACCCGGTAC 
TCTGTGTTCC ACGACCTCAT CCGCGCCACG CTGAAGGTCA ACCCCGAGGA GCGGCTGTCC 
ATCACAGAAC TGGTGAACCA GCTCCAGGAG ATCGCGGCCG CCAGGAACGT GAACCCCAAG 
TCTCCCATCA CTGAGCTCCT AGAGCAGAAC GGAGGCTATG GGAACGCTGT CCCTTCCCGA 
GGGCCACCCC CTCCTGGGGG CTCCAGCAGC GGCTACAGCG GAGGCCTGGC GCTGGCCGAG 
TACGACCAGC CCTACGGCGG GCTCCTGGAC ATCCTGCGGG GGGGCACGGA GCGCCTCTTC 
ACAAACCTCA AGGACACCTC CTCCAAGGTC ATCCAGTCTG TTGCCAATTA CGCAAAAGGA 
GATTTGGACA TCTCTTACAT CACATCTAGA ATTGCTGTGA TGTCGTTCCC AGCAGAAGGC 
GTGGAGTCGG CCATCAAGAA CAACATCGAA GACGTGCGGT TGTTTCTGGA CTCCAAGCAC 
CCGGGACATT ACGCCGTCTA CAACCTGTCC CCAAGAACAT ACCGGCCCTC CAAATTTCAC 
AACCGGGTCT CCGAGTGTGG CTGGGCGGCC AGACGGGCCC CGAACCTTCG CAGCCTGTAC 
AGTGTGTGTC GGAACATGCA CTCGTGGCTC CGGCAGGACC ACAAGAACGT CTGTGTCGTG 
CACTGCATGG ACGGGAGAGC TGCGTCTGCG GTGGCCGTGT GCTCCTTCCT GTGCTTCTGC 
CGCCTGTTCA GCACCGCGGA GGCTGCCGTG TACATGTTCA GCATGAAGCG CTGCCCGCCA 
GGCATTTGGC CATCCCACAA GAGGTACATC GAGTACATGT GTGACATGGT GGCCGAGGAG 
CCCATCACAC CCCATAGCAA GCCCATCCTG GTGAAAGCCG TGGTCATGAC ACCCGTGCCC 
CTGTTCAGCA AGCAGAGAAA CGGCTGCAGG CCCTTCTGCG AGGTCTACGT GGGGGATGAG 
CGCGTGACTA CCACGTCTCA GGAGTACGAC AAGATGAGGG ATTTCAGAAT CGAGGATGGC 
AAAGCAGTCA TTCCCCTGGG CATAACAGTG CAAGGGGACG TGCTCATTGT CATCTACCAC 
GCCAGGTCCA CCCTGGGAGG AAGACTGCAG GCCAAGGTGG CATCCATGAA GATGTTCCAG 
GTCCAGTTCC ACACGGGGTT TGTGCCTCGG AATGCGACTA CTGTGAAGTT TGCAAAGTAT 
GACCTTGATG CGTGTGACAT TCAGGAGAAA TACCCAGATT TATTCCAAGT GAACGTGGAG 
GTGGAGGTGG AGCCCAGAGA CAGGCCCAGC CGGGAGGCCC CACCCTGGGA GAGCACCAGC 
ATGAGGGGGC TCAACCCCAA AATCCTGTTT TCCAGCCGAG AGGAGCAGCA AGACATTCTG 
TCTAAATTTG GGAAGCCGGA GCTCCCCAGG CAGCCCGGCT CCACCGCTCA GTACGACGCC 
GAGGCAGGGT CCCTGGACGC AGAGCCCACA GAGTCTGATT CACCACAGAG CAGCGGCACT 
GACACCAGCC ACTTCCTCCA CACGCTGGAT TGGCACGAGG AGAAAGATAC AGAGACGAGT 
CCAGAAAGTC ACTTTGCCAA GGAGAACCTA TCAACCCTGG CCGAGGATGC AGGTGGGAGT 
GAGCCTTCGG ACGAGGAAGC AGCCACACCA TCCACCGAGA GCAGGGATGC TGCGGATGAG 
GACACACCAG GGCCAGCGGC GAGGCTGCCA GAGCGAGATT TGATTTTTGA TGGGGCCACG 
CCGGCCACAC CGCAGGAGCC CACACGGCAG GAGGACAGTG TCGACCTCCT AGGGCTGCAT 
GCCGAGGCTG GGCTGGCCCC CCCTGTGCAG GCCAGTGGGG CAGCCCCCAG CACCGCCGAC 
CTGCTCAGCC GCCTCCTGGG GGGCCCTGAC GCTGCTCCGG AGGTGCCCCC GGGTGACCTG 
CTGGGTGGAG AGGCTCCCCT CCTCTTCGCA AGCCCAGCCC CCTCCTCGAG CGCACAGAGC 
CCGCCCCGTG AGGGGCCAGC TGCCGACCCC TTTGACCCTC TCCTGCTGCC ATCTGGGCCC 
AACACCCAGC CGTGCTCCCA GCCTGACCTG TTCGGTGAAT TTCTTCATTC GGAATCTCCT 
GCCGCCGTGC CTGCGTCCTT CCCGTCCACC CACAGCGCCC CACCGCCCGC CTGCAGCACC 
GACTTCCTGC ACCTGGGAGA TCTACCAGCA GAGCCTGGCA AGATGACGGC CTCCACCAGC 
CACCCGGATC TGCTGGGGGG GTGGGAGGCC TGGGCCGAGG CGCCGGCCCC CGCAGCTGCT 
GCAGAAGGTC CCTTCTTCTC TGCAGGAGGC CAGCCTGCCG CCCCCAGTGC CCCCGGGAGC 
TGGACCAAGT CTCAGAGCCT GGACCCGTTT GCTGACCTCG GTGACCTCAG TTCTGGCCTC 
CAAGGCTCAC CGGCTGGATT TGCTCCAGGG GCTTTTGGCG CCAAGACGGC CCCCCCTCCC 
ACGGGCGGCA GCTCCTGGCA GCAGACGGGT CGGCCCCCGG CCCAGGGCAC CCCGTGGCCC 
CCCCAGGCCA AGCCGCCCCC CAAGGCCTGT CCGCAGCCAA GGCCTAACTA TGCTGCCAAC 
GTGAGCGTGA TTGGCGGCCG AGAGGAGAGA GGGGTCCGGG CCCCCAGCTT CGGCCAGAAG 
CCGAGAGTGT CCGAGAGCGA CTTTGAGGAC CTGCTGTCCC ACCAGGGCTT CTCGTCCAAG 
GCTGACCGGA AGGGGCCAAG GACCATGGCT GAGATGCGGC GGCAGGAGCA GGCGAGGGAC 
GCAGACCCGC TGAAGCTGAA GCTCCTGGAG TGGACAGAGG GCAAGGAGAG GAACATCCGC 
GCGCTGCTGT CGACCCTGCA CACCGTGCTG TGGGACGGCG AGAGCCGCTG GGCCCCCGTG 
GGCATGGCCG ACCTGGTGAC CCCCGGGCAG GTGAAGAAGC AGTACCGCCG CGCCGTGCTG 
GTGGTGCACC CGGACAAGGC CGCAGGGCAG CCCTACGAGC AGTATGCCAA GATGATCTTC 
ATGGAGCTGA ACGACGCGTG GGCCGAGTTT GAGAGCCAGG GCTCCCGGCC GCTCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022272995.1
CDS	230..3949
Translation	MPSVCCPAWPVVRLTHSPLTRHLQKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLTELCKGQLVDFLKKIESRGPLSCDTVLKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKFSIPPNDTRYSVFHDLIRATLKVNPEERLSITELVNQLQEIAAARNVNPKSPITELLEQNGGYGNAVPSRGPPPPGGSSSGYSGGLALAEYDQPYGGLLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRLFLDSKHPGHYAVYNLSPRTYRPSKFHNRVSECGWAARRAPNLRSLYSVCRNMHSWLRQDHKNVCVVHCMDGRAASAVAVCSFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYMCDMVAEEPITPHSKPILVKAVVMTPVPLFSKQRNGCRPFCEVYVGDERVTTTSQEYDKMRDFRIEDGKAVIPLGITVQGDVLIVIYHARSTLGGRLQAKVASMKMFQVQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNVEVEVEPRDRPSREAPPWESTSMRGLNPKILFSSREEQQDILSKFGKPELPRQPGSTAQYDAEAGSLDAEPTESDSPQSSGTDTSHFLHTLDWHEEKDTETSPESHFAKENLSTLAEDAGGSEPSDEEAATPSTESRDAADEDTPGPAARLPERDLIFDGATPATPQEPTRQEDSVDLLGLHAEAGLAPPVQASGAAPSTADLLSRLLGGPDAAPEVPPGDLLGGEAPLLFASPAPSSSAQSPPREGPAADPFDPLLLPSGPNTQPCSQPDLFGEFLHSESPAAVPASFPSTHSAPPPACSTDFLHLGDLPAEPGKMTASTSHPDLLGGWEAWAEAPAPAAAAEGPFFSAGGQPAAPSAPGSWTKSQSLDPFADLGDLSSGLQGSPAGFAPGAFGAKTAPPPTGGSSWQQTGRPPAQGTPWPPQAKPPPKACPQPRPNYAANVSVIGGREERGVRAPSFGQKPRVSESDFEDLLSHQGFSSKADRKGPRTMAEMRRQEQARDADPLKLKLLEWTEGKERNIRALLSTLHTVLWDGESRWAPVGMADLVTPGQVKKQYRRAVLVVHPDKAAGQPYEQYAKMIFMELNDAWAEFESQGSRPLF

Target ORF information:

RefSeq Version	XM_022417287.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022417287.1

ORF Insert Sequence:

ATGCCCTCTG TCTGCTGTCC TGCCTGGCCT GTGGTCAGAC TTACGCATTC TCCTTTAACA 
AGACACCTGC AGAAAAAACT TTCTGGCCAC CCCAATATTG TCCAGTTTTG TTCTGCAGCG 
TCCATAGGGA AAGAGGAGTC GGACACTGGG CAGGCTGAGT TCCTGCTGCT CACCGAGCTC 
TGCAAAGGGC AGCTGGTGGA CTTTTTAAAG AAAATTGAAT CTAGAGGTCC GCTCTCGTGC 
GATACTGTTC TGAAGATTTT CTATCAGACG TGCAGAGCGG TGCAGCACAT GCACAGACAA 
AAGCCGCCCA TCATCCATAG AGACCTCAAG GTGGAAAACT TATTGCTCAG TAACCAAGGG 
ACCATCAAGC TGTGTGACTT TGGCAGTGCC ACGACCATAT CACATTACCC CGACTACAGC 
TGGAGCGCCC AGAGGCGGGC CCTGGTGGAG GAAGAGATCA CCAGGAACAC CACACCCATG 
TACAGGACAC CGGAAATCGT GGACTTGTAC TCCAACTTCC CCATCGGCGA GAAGCAGGAC 
ATCTGGGCGC TGGGCTGCAT CTTGTATCTG CTGTGCTTCC GGCAGCATCC TTTTGAAGAC 
GGAGCAAAGC TTCGGATAGT CAACGGGAAG TTCTCGATCC CCCCCAATGA CACCCGGTAC 
TCTGTGTTCC ACGACCTCAT CCGCGCCACG CTGAAGGTCA ACCCCGAGGA GCGGCTGTCC 
ATCACAGAAC TGGTGAACCA GCTCCAGGAG ATCGCGGCCG CCAGGAACGT GAACCCCAAG 
TCTCCCATCA CTGAGCTCCT AGAGCAGAAC GGAGGCTATG GGAACGCTGT CCCTTCCCGA 
GGGCCACCCC CTCCTGGGGG CTCCAGCAGC GGCTACAGCG GAGGCCTGGC GCTGGCCGAG 
TACGACCAGC CCTACGGCGG GCTCCTGGAC ATCCTGCGGG GGGGCACGGA GCGCCTCTTC 
ACAAACCTCA AGGACACCTC CTCCAAGGTC ATCCAGTCTG TTGCCAATTA CGCAAAAGGA 
GATTTGGACA TCTCTTACAT CACATCTAGA ATTGCTGTGA TGTCGTTCCC AGCAGAAGGC 
GTGGAGTCGG CCATCAAGAA CAACATCGAA GACGTGCGGT TGTTTCTGGA CTCCAAGCAC 
CCGGGACATT ACGCCGTCTA CAACCTGTCC CCAAGAACAT ACCGGCCCTC CAAATTTCAC 
AACCGGGTCT CCGAGTGTGG CTGGGCGGCC AGACGGGCCC CGAACCTTCG CAGCCTGTAC 
AGTGTGTGTC GGAACATGCA CTCGTGGCTC CGGCAGGACC ACAAGAACGT CTGTGTCGTG 
CACTGCATGG ACGGGAGAGC TGCGTCTGCG GTGGCCGTGT GCTCCTTCCT GTGCTTCTGC 
CGCCTGTTCA GCACCGCGGA GGCTGCCGTG TACATGTTCA GCATGAAGCG CTGCCCGCCA 
GGCATTTGGC CATCCCACAA GAGGTACATC GAGTACATGT GTGACATGGT GGCCGAGGAG 
CCCATCACAC CCCATAGCAA GCCCATCCTG GTGAAAGCCG TGGTCATGAC ACCCGTGCCC 
CTGTTCAGCA AGCAGAGAAA CGGCTGCAGG CCCTTCTGCG AGGTCTACGT GGGGGATGAG 
CGCGTGACTA CCACGTCTCA GGAGTACGAC AAGATGAGGG ATTTCAGAAT CGAGGATGGC 
AAAGCAGTCA TTCCCCTGGG CATAACAGTG CAAGGGGACG TGCTCATTGT CATCTACCAC 
GCCAGGTCCA CCCTGGGAGG AAGACTGCAG GCCAAGGTGG CATCCATGAA GATGTTCCAG 
GTCCAGTTCC ACACGGGGTT TGTGCCTCGG AATGCGACTA CTGTGAAGTT TGCAAAGTAT 
GACCTTGATG CGTGTGACAT TCAGGAGAAA TACCCAGATT TATTCCAAGT GAACGTGGAG 
GTGGAGGTGG AGCCCAGAGA CAGGCCCAGC CGGGAGGCCC CACCCTGGGA GAGCACCAGC 
ATGAGGGGGC TCAACCCCAA AATCCTGTTT TCCAGCCGAG AGGAGCAGCA AGACATTCTG 
TCTAAATTTG GGAAGCCGGA GCTCCCCAGG CAGCCCGGCT CCACCGCTCA GTACGACGCC 
GAGGCAGGGT CCCTGGACGC AGAGCCCACA GAGTCTGATT CACCACAGAG CAGCGGCACT 
GACACCAGCC ACTTCCTCCA CACGCTGGAT TGGCACGAGG AGAAAGATAC AGAGACGAGT 
CCAGAAAGTC ACTTTGCCAA GGAGAACCTA TCAACCCTGG CCGAGGATGC AGGTGGGAGT 
GAGCCTTCGG ACGAGGAAGC AGCCACACCA TCCACCGAGA GCAGGGATGC TGCGGATGAG 
GACACACCAG GGCCAGCGGC GAGGCTGCCA GAGCGAGATT TGATTTTTGA TGGGGCCACG 
CCGGCCACAC CGCAGGAGCC CACACGGCAG GAGGACAGTG TCGACCTCCT AGGGCTGCAT 
GCCGAGGCTG GGCTGGCCCC CCCTGTGCAG GCCAGTGGGG CAGCCCCCAG CACCGCCGAC 
CTGCTCAGCC GCCTCCTGGG GGGCCCTGAC GCTGCTCCGG AGGTGCCCCC GGGTGACCTG 
CTGGGTGGAG AGGCTCCCCT CCTCTTCGCA AGCCCAGCCC CCTCCTCGAG CGCACAGAGC 
CCGCCCCGTG AGGGGCCAGC TGCCGACCCC TTTGACCCTC TCCTGCTGCC ATCTGGGCCC 
AACACCCAGC CGTGCTCCCA GCCTGACCTG TTCGGTGAAT TTCTTCATTC GGAATCTCCT 
GCCGCCGTGC CTGCGTCCTT CCCGTCCACC CACAGCGCCC CACCGCCCGC CTGCAGCACC 
GACTTCCTGC ACCTGGGAGA TCTACCAGCA GAGCCTGGCA AGATGACGGC CTCCACCAGC 
CACCCGGATC TGCTGGGGGG GTGGGAGGCC TGGGCCGAGG CGCCGGCCCC CGCAGCTGCT 
GCAGAAGGTC CCTTCTTCTC TGCAGGAGGC CAGCCTGCCG CCCCCAGTGC CCCCGGGAGC 
TGGACCAAGT CTCAGAGCCT GGACCCGTTT GCTGACCTCG GTGACCTCAG TTCTGGCCTC 
CAAGGCTCAC CGGCTGGATT TGCTCCAGGG GCTTTTGGCG CCAAGACGGC CCCCCCTCCC 
ACGGGCGGCA GCTCCTGGCA GCAGACGGGT CGGCCCCCGG CCCAGGGCAC CCCGTGGCCC 
CCCCAGGCCA AGCCGCCCCC CAAGGCCTGT CCGCAGCCAA GGCCTAACTA TGCTGCCAAC 
GTGAGCGTGA TTGGCGGCCG AGAGGAGAGA GGGGTCCGGG CCCCCAGCTT CGGCCAGAAG 
CCGAGAGTGT CCGAGAGCGA CTTTGAGGAC CTGCTGTCCC ACCAGGGCTT CTCGTCCAAG 
GCTGACCGGA AGGGGCCAAG GACCATGGCT GAGATGCGGC GGCAGGAGCA GGCGAGGGAC 
GCAGACCCGC TGAAGCTGAA GCTCCTGGAG TGGACAGAGG GCAAGGAGAG GAACATCCGC 
GCGCTGCTGT CGACCCTGCA CACCGTGCTG TGGGACGGCG AGAGCCGCTG GGCCCCCGTG 
GGCATGGCCG ACCTGGTGAC CCCCGGGCAG GTGAAGAAGC AGTACCGCCG CGCCGTGCTG 
GTGGTGCACC CGGACAAGGC CGCAGGGCAG CCCTACGAGC AGTATGCCAA GATGATCTTC 
ATGGAGCTGA ACGACGCGTG GGCCGAGTTT GAGAGCCAGG GCTCCCGGCC GCTCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAK ( XM_005618687.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005618744.1 Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe04653
Clone ID Related Accession (Same CDS sequence)	XM_005618687.2 , XM_005618689.2 , XM_005618687.3 , XM_022417295.1 , XM_005618689.3 , XM_022417288.1
Accession Version	XM_005618687.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	cyclin-G-associated kinase isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005618687.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAAC TTTCTGGCCA CCCCAATATT GTCCAGTTTT GTTCTGCAGC GTCCATAGGG 
AAAGAGGAGT CGGACACTGG GCAGGCTGAG TTCCTGCTGC TCACCGAGCT CTGCAAAGGG 
CAGCTGGTGG ACTTTTTAAA GAAAATTGAA TCTAGAGGTC CGCTCTCGTG CGATACTGTT 
CTGAAGATTT TCTATCAGAC GTGCAGAGCG GTGCAGCACA TGCACAGACA AAAGCCGCCC 
ATCATCCATA GAGACCTCAA GGTGGAAAAC TTATTGCTCA GTAACCAAGG GACCATCAAG 
CTGTGTGACT TTGGCAGTGC CACGACCATA TCACATTACC CCGACTACAG CTGGAGCGCC 
CAGAGGCGGG CCCTGGTGGA GGAAGAGATC ACCAGGAACA CCACACCCAT GTACAGGACA 
CCGGAAATCG TGGACTTGTA CTCCAACTTC CCCATCGGCG AGAAGCAGGA CATCTGGGCG 
CTGGGCTGCA TCTTGTATCT GCTGTGCTTC CGGCAGCATC CTTTTGAAGA CGGAGCAAAG 
CTTCGGATAG TCAACGGGAA GTTCTCGATC CCCCCCAATG ACACCCGGTA CTCTGTGTTC 
CACGACCTCA TCCGCGCCAC GCTGAAGGTC AACCCCGAGG AGCGGCTGTC CATCACAGAA 
CTGGTGAACC AGCTCCAGGA GATCGCGGCC GCCAGGAACG TGAACCCCAA GTCTCCCATC 
ACTGAGCTCC TAGAGCAGAA CGGAGGCTAT GGGAACGCTG TCCCTTCCCG AGGGCCACCC 
CCTCCTGGGG GCTCCAGCAG CGGCTACAGC GGAGGCCTGG CGCTGGCCGA GTACGACCAG 
CCCTACGGCG GGCTCCTGGA CATCCTGCGG GGGGGCACGG AGCGCCTCTT CACAAACCTC 
AAGGACACCT CCTCCAAGGT CATCCAGTCT GTTGCCAATT ACGCAAAAGG AGATTTGGAC 
ATCTCTTACA TCACATCTAG AATTGCTGTG ATGTCGTTCC CAGCAGAAGG CGTGGAGTCG 
GCCATCAAGA ACAACATCGA AGACGTGCGG TTGTTTCTGG ACTCCAAGCA CCCGGGACAT 
TACGCCGTCT ACAACCTGTC CCCAAGAACA TACCGGCCCT CCAAATTTCA CAACCGGGTC 
TCCGAGTGTG GCTGGGCGGC CAGACGGGCC CCGAACCTTC GCAGCCTGTA CAGTGTGTGT 
CGGAACATGC ACTCGTGGCT CCGGCAGGAC CACAAGAACG TCTGTGTCGT GCACTGCATG 
GACGGGAGAG CTGCGTCTGC GGTGGCCGTG TGCTCCTTCC TGTGCTTCTG CCGCCTGTTC 
AGCACCGCGG AGGCTGCCGT GTACATGTTC AGCATGAAGC GCTGCCCGCC AGGCATTTGG 
CCATCCCACA AGAGGTACAT CGAGTACATG TGTGACATGG TGGCCGAGGA GCCCATCACA 
CCCCATAGCA AGCCCATCCT GGTGAAAGCC GTGGTCATGA CACCCGTGCC CCTGTTCAGC 
AAGCAGAGAA ACGGCTGCAG GCCCTTCTGC GAGGTCTACG TGGGGGATGA GCGCGTGACT 
ACCACGTCTC AGGAGTACGA CAAGATGAGG GATTTCAGAA TCGAGGATGG CAAAGCAGTC 
ATTCCCCTGG GCATAACAGT GCAAGGGGAC GTGCTCATTG TCATCTACCA CGCCAGGTCC 
ACCCTGGGAG GAAGACTGCA GGCCAAGGTG GCATCCATGA AGATGTTCCA GGTCCAGTTC 
CACACGGGGT TTGTGCCTCG GAATGCGACT ACTGTGAAGT TTGCAAAGTA TGACCTTGAT 
GCGTGTGACA TTCAGGAGAA ATACCCAGAT TTATTCCAAG TGAACGTGGA GGTGGAGGTG 
GAGCCCAGAG ACAGGCCCAG CCGGGAGGCC CCACCCTGGG AGAGCACCAG CATGAGGGGG 
CTCAACCCCA AAATCCTGTT TTCCAGCCGA GAGGAGCAGC AAGACATTCT GTCTAAATTT 
GGGAAGCCGG AGCTCCCCAG GCAGCCCGGC TCCACCGCTC AGTACGACGC CGAGGCAGGG 
TCCCTGGACG CAGAGCCCAC AGAGTCTGAT TCACCACAGA GCAGCGGCAC TGACACCAGC 
CACTTCCTCC ACACGCTGGA TTGGCACGAG GAGAAAGATA CAGAGACGAG TCCAGAAAGT 
CACTTTGCCA AGGAGAACCT ATCAACCCTG GCCGAGGATG CAGGTGGGAG TGAGCCTTCG 
GACGAGGAAG CAGCCACACC ATCCACCGAG AGCAGGGATG CTGCGGATGA GGACACACCA 
GGGCCAGCGG CGAGGCTGCC AGAGCGAGAT TTGATTTTTG ATGGGGCCAC GCCGGCCACA 
CCGCAGGAGC CCACACGGCA GGAGGACAGT GTCGACCTCC TAGGGCTGCA TGCCGAGGCT 
GGGCTGGCCC CCCCTGTGCA GGCCAGTGGG GCAGCCCCCA GCACCGCCGA CCTGCTCAGC 
CGCCTCCTGG GGGGCCCTGA CGCTGCTCCG GAGGTGCCCC CGGGTGACCT GCTGGGTGGA 
GAGGCTCCCC TCCTCTTCGC AAGCCCAGCC CCCTCCTCGA GCGCACAGAG CCCGCCCCGT 
GAGGGGCCAG CTGCCGACCC CTTTGACCCT CTCCTGCTGC CATCTGGGCC CAACACCCAG 
CCGTGCTCCC AGCCTGACCT GTTCGGTGAA TTTCTTCATT CGGAATCTCC TGCCGCCGTG 
CCTGCGTCCT TCCCGTCCAC CCACAGCGCC CCACCGCCCG CCTGCAGCAC CGACTTCCTG 
CACCTGGGAG ATCTACCAGC AGAGCCTGGC AAGATGACGG CCTCCACCAG CCACCCGGAT 
CTGCTGGGGG GGTGGGAGGC CTGGGCCGAG GCGCCGGCCC CCGCAGCTGC TGCAGAAGGT 
CCCTTCTTCT CTGCAGGAGG CCAGCCTGCC GCCCCCAGTG CCCCCGGGAG CTGGACCAAG 
TCTCAGAGCC TGGACCCGTT TGCTGACCTC GGTGACCTCA GTTCTGGCCT CCAAGGCTCA 
CCGGCTGGAT TTGCTCCAGG GGCTTTTGGC GCCAAGACGG CCCCCCCTCC CACGGGCGGC 
AGCTCCTGGC AGCAGACGGG TCGGCCCCCG GCCCAGGGCA CCCCGTGGCC CCCCCAGGCC 
AAGCCGCCCC CCAAGGCCTG TCCGCAGCCA AGGCCTAACT ATGCTGCCAA CGTGAGCGTG 
ATTGGCGGCC GAGAGGAGAG AGGGGTCCGG GCCCCCAGCT TCGGCCAGAA GCCGAGAGTG 
TCCGAGAGCG ACTTTGAGGA CCTGCTGTCC CACCAGGGCT TCTCGTCCAA GGCTGACCGG 
AAGGGGCCAA GGACCATGGC TGAGATGCGG CGGCAGGAGC AGGCGAGGGA CGCAGACCCG 
CTGAAGCTGA AGCTCCTGGA GTGGACAGAG GGCAAGGAGA GGAACATCCG CGCGCTGCTG 
TCGACCCTGC ACACCGTGCT GTGGGACGGC GAGAGCCGCT GGGCCCCCGT GGGCATGGCC 
GACCTGGTGA CCCCCGGGCA GGTGAAGAAG CAGTACCGCC GCGCCGTGCT GGTGGTGCAC 
CCGGACAAGG CCGCAGGGCA GCCCTACGAG CAGTATGCCA AGATGATCTT CATGGAGCTG 
AACGACGCGT GGGCCGAGTT TGAGAGCCAG GGCTCCCGGC CGCTCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005618744.1
CDS	229..3879
Translation	MKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLTELCKGQLVDFLKKIESRGPLSCDTVLKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKFSIPPNDTRYSVFHDLIRATLKVNPEERLSITELVNQLQEIAAARNVNPKSPITELLEQNGGYGNAVPSRGPPPPGGSSSGYSGGLALAEYDQPYGGLLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRLFLDSKHPGHYAVYNLSPRTYRPSKFHNRVSECGWAARRAPNLRSLYSVCRNMHSWLRQDHKNVCVVHCMDGRAASAVAVCSFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYMCDMVAEEPITPHSKPILVKAVVMTPVPLFSKQRNGCRPFCEVYVGDERVTTTSQEYDKMRDFRIEDGKAVIPLGITVQGDVLIVIYHARSTLGGRLQAKVASMKMFQVQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNVEVEVEPRDRPSREAPPWESTSMRGLNPKILFSSREEQQDILSKFGKPELPRQPGSTAQYDAEAGSLDAEPTESDSPQSSGTDTSHFLHTLDWHEEKDTETSPESHFAKENLSTLAEDAGGSEPSDEEAATPSTESRDAADEDTPGPAARLPERDLIFDGATPATPQEPTRQEDSVDLLGLHAEAGLAPPVQASGAAPSTADLLSRLLGGPDAAPEVPPGDLLGGEAPLLFASPAPSSSAQSPPREGPAADPFDPLLLPSGPNTQPCSQPDLFGEFLHSESPAAVPASFPSTHSAPPPACSTDFLHLGDLPAEPGKMTASTSHPDLLGGWEAWAEAPAPAAAAEGPFFSAGGQPAAPSAPGSWTKSQSLDPFADLGDLSSGLQGSPAGFAPGAFGAKTAPPPTGGSSWQQTGRPPAQGTPWPPQAKPPPKACPQPRPNYAANVSVIGGREERGVRAPSFGQKPRVSESDFEDLLSHQGFSSKADRKGPRTMAEMRRQEQARDADPLKLKLLEWTEGKERNIRALLSTLHTVLWDGESRWAPVGMADLVTPGQVKKQYRRAVLVVHPDKAAGQPYEQYAKMIFMELNDAWAEFESQGSRPLF

Target ORF information:

RefSeq Version	XM_005618687.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005618687.2

ORF Insert Sequence:

ATGAAAAAAC TTTCTGGCCA CCCCAATATT GTCCAGTTTT GTTCTGCAGC GTCCATAGGG 
AAAGAGGAGT CGGACACTGG GCAGGCTGAG TTCCTGCTGC TCACCGAGCT CTGCAAAGGG 
CAGCTGGTGG ACTTTTTAAA GAAAATTGAA TCTAGAGGTC CGCTCTCGTG CGATACTGTT 
CTGAAGATTT TCTATCAGAC GTGCAGAGCG GTGCAGCACA TGCACAGACA AAAGCCGCCC 
ATCATCCATA GAGACCTCAA GGTGGAAAAC TTATTGCTCA GTAACCAAGG GACCATCAAG 
CTGTGTGACT TTGGCAGTGC CACGACCATA TCACATTACC CCGACTACAG CTGGAGCGCC 
CAGAGGCGGG CCCTGGTGGA GGAAGAGATC ACCAGGAACA CCACACCCAT GTACAGGACA 
CCGGAAATCG TGGACTTGTA CTCCAACTTC CCCATCGGCG AGAAGCAGGA CATCTGGGCG 
CTGGGCTGCA TCTTGTATCT GCTGTGCTTC CGGCAGCATC CTTTTGAAGA CGGAGCAAAG 
CTTCGGATAG TCAACGGGAA GTTCTCGATC CCCCCCAATG ACACCCGGTA CTCTGTGTTC 
CACGACCTCA TCCGCGCCAC GCTGAAGGTC AACCCCGAGG AGCGGCTGTC CATCACAGAA 
CTGGTGAACC AGCTCCAGGA GATCGCGGCC GCCAGGAACG TGAACCCCAA GTCTCCCATC 
ACTGAGCTCC TAGAGCAGAA CGGAGGCTAT GGGAACGCTG TCCCTTCCCG AGGGCCACCC 
CCTCCTGGGG GCTCCAGCAG CGGCTACAGC GGAGGCCTGG CGCTGGCCGA GTACGACCAG 
CCCTACGGCG GGCTCCTGGA CATCCTGCGG GGGGGCACGG AGCGCCTCTT CACAAACCTC 
AAGGACACCT CCTCCAAGGT CATCCAGTCT GTTGCCAATT ACGCAAAAGG AGATTTGGAC 
ATCTCTTACA TCACATCTAG AATTGCTGTG ATGTCGTTCC CAGCAGAAGG CGTGGAGTCG 
GCCATCAAGA ACAACATCGA AGACGTGCGG TTGTTTCTGG ACTCCAAGCA CCCGGGACAT 
TACGCCGTCT ACAACCTGTC CCCAAGAACA TACCGGCCCT CCAAATTTCA CAACCGGGTC 
TCCGAGTGTG GCTGGGCGGC CAGACGGGCC CCGAACCTTC GCAGCCTGTA CAGTGTGTGT 
CGGAACATGC ACTCGTGGCT CCGGCAGGAC CACAAGAACG TCTGTGTCGT GCACTGCATG 
GACGGGAGAG CTGCGTCTGC GGTGGCCGTG TGCTCCTTCC TGTGCTTCTG CCGCCTGTTC 
AGCACCGCGG AGGCTGCCGT GTACATGTTC AGCATGAAGC GCTGCCCGCC AGGCATTTGG 
CCATCCCACA AGAGGTACAT CGAGTACATG TGTGACATGG TGGCCGAGGA GCCCATCACA 
CCCCATAGCA AGCCCATCCT GGTGAAAGCC GTGGTCATGA CACCCGTGCC CCTGTTCAGC 
AAGCAGAGAA ACGGCTGCAG GCCCTTCTGC GAGGTCTACG TGGGGGATGA GCGCGTGACT 
ACCACGTCTC AGGAGTACGA CAAGATGAGG GATTTCAGAA TCGAGGATGG CAAAGCAGTC 
ATTCCCCTGG GCATAACAGT GCAAGGGGAC GTGCTCATTG TCATCTACCA CGCCAGGTCC 
ACCCTGGGAG GAAGACTGCA GGCCAAGGTG GCATCCATGA AGATGTTCCA GGTCCAGTTC 
CACACGGGGT TTGTGCCTCG GAATGCGACT ACTGTGAAGT TTGCAAAGTA TGACCTTGAT 
GCGTGTGACA TTCAGGAGAA ATACCCAGAT TTATTCCAAG TGAACGTGGA GGTGGAGGTG 
GAGCCCAGAG ACAGGCCCAG CCGGGAGGCC CCACCCTGGG AGAGCACCAG CATGAGGGGG 
CTCAACCCCA AAATCCTGTT TTCCAGCCGA GAGGAGCAGC AAGACATTCT GTCTAAATTT 
GGGAAGCCGG AGCTCCCCAG GCAGCCCGGC TCCACCGCTC AGTACGACGC CGAGGCAGGG 
TCCCTGGACG CAGAGCCCAC AGAGTCTGAT TCACCACAGA GCAGCGGCAC TGACACCAGC 
CACTTCCTCC ACACGCTGGA TTGGCACGAG GAGAAAGATA CAGAGACGAG TCCAGAAAGT 
CACTTTGCCA AGGAGAACCT ATCAACCCTG GCCGAGGATG CAGGTGGGAG TGAGCCTTCG 
GACGAGGAAG CAGCCACACC ATCCACCGAG AGCAGGGATG CTGCGGATGA GGACACACCA 
GGGCCAGCGG CGAGGCTGCC AGAGCGAGAT TTGATTTTTG ATGGGGCCAC GCCGGCCACA 
CCGCAGGAGC CCACACGGCA GGAGGACAGT GTCGACCTCC TAGGGCTGCA TGCCGAGGCT 
GGGCTGGCCC CCCCTGTGCA GGCCAGTGGG GCAGCCCCCA GCACCGCCGA CCTGCTCAGC 
CGCCTCCTGG GGGGCCCTGA CGCTGCTCCG GAGGTGCCCC CGGGTGACCT GCTGGGTGGA 
GAGGCTCCCC TCCTCTTCGC AAGCCCAGCC CCCTCCTCGA GCGCACAGAG CCCGCCCCGT 
GAGGGGCCAG CTGCCGACCC CTTTGACCCT CTCCTGCTGC CATCTGGGCC CAACACCCAG 
CCGTGCTCCC AGCCTGACCT GTTCGGTGAA TTTCTTCATT CGGAATCTCC TGCCGCCGTG 
CCTGCGTCCT TCCCGTCCAC CCACAGCGCC CCACCGCCCG CCTGCAGCAC CGACTTCCTG 
CACCTGGGAG ATCTACCAGC AGAGCCTGGC AAGATGACGG CCTCCACCAG CCACCCGGAT 
CTGCTGGGGG GGTGGGAGGC CTGGGCCGAG GCGCCGGCCC CCGCAGCTGC TGCAGAAGGT 
CCCTTCTTCT CTGCAGGAGG CCAGCCTGCC GCCCCCAGTG CCCCCGGGAG CTGGACCAAG 
TCTCAGAGCC TGGACCCGTT TGCTGACCTC GGTGACCTCA GTTCTGGCCT CCAAGGCTCA 
CCGGCTGGAT TTGCTCCAGG GGCTTTTGGC GCCAAGACGG CCCCCCCTCC CACGGGCGGC 
AGCTCCTGGC AGCAGACGGG TCGGCCCCCG GCCCAGGGCA CCCCGTGGCC CCCCCAGGCC 
AAGCCGCCCC CCAAGGCCTG TCCGCAGCCA AGGCCTAACT ATGCTGCCAA CGTGAGCGTG 
ATTGGCGGCC GAGAGGAGAG AGGGGTCCGG GCCCCCAGCT TCGGCCAGAA GCCGAGAGTG 
TCCGAGAGCG ACTTTGAGGA CCTGCTGTCC CACCAGGGCT TCTCGTCCAA GGCTGACCGG 
AAGGGGCCAA GGACCATGGC TGAGATGCGG CGGCAGGAGC AGGCGAGGGA CGCAGACCCG 
CTGAAGCTGA AGCTCCTGGA GTGGACAGAG GGCAAGGAGA GGAACATCCG CGCGCTGCTG 
TCGACCCTGC ACACCGTGCT GTGGGACGGC GAGAGCCGCT GGGCCCCCGT GGGCATGGCC 
GACCTGGTGA CCCCCGGGCA GGTGAAGAAG CAGTACCGCC GCGCCGTGCT GGTGGTGCAC 
CCGGACAAGG CCGCAGGGCA GCCCTACGAG CAGTATGCCA AGATGATCTT CATGGAGCTG 
AACGACGCGT GGGCCGAGTT TGAGAGCCAG GGCTCCCGGC CGCTCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAK ( XM_005618689.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005618746.1 Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OCe04653
Clone ID Related Accession (Same CDS sequence)	XM_005618687.2 , XM_005618689.2 , XM_005618687.3 , XM_022417295.1 , XM_005618689.3 , XM_022417288.1
Accession Version	XM_005618689.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	cyclin-G-associated kinase isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005618689.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAAC TTTCTGGCCA CCCCAATATT GTCCAGTTTT GTTCTGCAGC GTCCATAGGG 
AAAGAGGAGT CGGACACTGG GCAGGCTGAG TTCCTGCTGC TCACCGAGCT CTGCAAAGGG 
CAGCTGGTGG ACTTTTTAAA GAAAATTGAA TCTAGAGGTC CGCTCTCGTG CGATACTGTT 
CTGAAGATTT TCTATCAGAC GTGCAGAGCG GTGCAGCACA TGCACAGACA AAAGCCGCCC 
ATCATCCATA GAGACCTCAA GGTGGAAAAC TTATTGCTCA GTAACCAAGG GACCATCAAG 
CTGTGTGACT TTGGCAGTGC CACGACCATA TCACATTACC CCGACTACAG CTGGAGCGCC 
CAGAGGCGGG CCCTGGTGGA GGAAGAGATC ACCAGGAACA CCACACCCAT GTACAGGACA 
CCGGAAATCG TGGACTTGTA CTCCAACTTC CCCATCGGCG AGAAGCAGGA CATCTGGGCG 
CTGGGCTGCA TCTTGTATCT GCTGTGCTTC CGGCAGCATC CTTTTGAAGA CGGAGCAAAG 
CTTCGGATAG TCAACGGGAA GTTCTCGATC CCCCCCAATG ACACCCGGTA CTCTGTGTTC 
CACGACCTCA TCCGCGCCAC GCTGAAGGTC AACCCCGAGG AGCGGCTGTC CATCACAGAA 
CTGGTGAACC AGCTCCAGGA GATCGCGGCC GCCAGGAACG TGAACCCCAA GTCTCCCATC 
ACTGAGCTCC TAGAGCAGAA CGGAGGCTAT GGGAACGCTG TCCCTTCCCG AGGGCCACCC 
CCTCCTGGGG GCTCCAGCAG CGGCTACAGC GGAGGCCTGG CGCTGGCCGA GTACGACCAG 
CCCTACGGCG GGCTCCTGGA CATCCTGCGG GGGGGCACGG AGCGCCTCTT CACAAACCTC 
AAGGACACCT CCTCCAAGGT CATCCAGTCT GTTGCCAATT ACGCAAAAGG AGATTTGGAC 
ATCTCTTACA TCACATCTAG AATTGCTGTG ATGTCGTTCC CAGCAGAAGG CGTGGAGTCG 
GCCATCAAGA ACAACATCGA AGACGTGCGG TTGTTTCTGG ACTCCAAGCA CCCGGGACAT 
TACGCCGTCT ACAACCTGTC CCCAAGAACA TACCGGCCCT CCAAATTTCA CAACCGGGTC 
TCCGAGTGTG GCTGGGCGGC CAGACGGGCC CCGAACCTTC GCAGCCTGTA CAGTGTGTGT 
CGGAACATGC ACTCGTGGCT CCGGCAGGAC CACAAGAACG TCTGTGTCGT GCACTGCATG 
GACGGGAGAG CTGCGTCTGC GGTGGCCGTG TGCTCCTTCC TGTGCTTCTG CCGCCTGTTC 
AGCACCGCGG AGGCTGCCGT GTACATGTTC AGCATGAAGC GCTGCCCGCC AGGCATTTGG 
CCATCCCACA AGAGGTACAT CGAGTACATG TGTGACATGG TGGCCGAGGA GCCCATCACA 
CCCCATAGCA AGCCCATCCT GGTGAAAGCC GTGGTCATGA CACCCGTGCC CCTGTTCAGC 
AAGCAGAGAA ACGGCTGCAG GCCCTTCTGC GAGGTCTACG TGGGGGATGA GCGCGTGACT 
ACCACGTCTC AGGAGTACGA CAAGATGAGG GATTTCAGAA TCGAGGATGG CAAAGCAGTC 
ATTCCCCTGG GCATAACAGT GCAAGGGGAC GTGCTCATTG TCATCTACCA CGCCAGGTCC 
ACCCTGGGAG GAAGACTGCA GGCCAAGGTG GCATCCATGA AGATGTTCCA GGTCCAGTTC 
CACACGGGGT TTGTGCCTCG GAATGCGACT ACTGTGAAGT TTGCAAAGTA TGACCTTGAT 
GCGTGTGACA TTCAGGAGAA ATACCCAGAT TTATTCCAAG TGAACGTGGA GGTGGAGGTG 
GAGCCCAGAG ACAGGCCCAG CCGGGAGGCC CCACCCTGGG AGAGCACCAG CATGAGGGGG 
CTCAACCCCA AAATCCTGTT TTCCAGCCGA GAGGAGCAGC AAGACATTCT GTCTAAATTT 
GGGAAGCCGG AGCTCCCCAG GCAGCCCGGC TCCACCGCTC AGTACGACGC CGAGGCAGGG 
TCCCTGGACG CAGAGCCCAC AGAGTCTGAT TCACCACAGA GCAGCGGCAC TGACACCAGC 
CACTTCCTCC ACACGCTGGA TTGGCACGAG GAGAAAGATA CAGAGACGAG TCCAGAAAGT 
CACTTTGCCA AGGAGAACCT ATCAACCCTG GCCGAGGATG CAGGTGGGAG TGAGCCTTCG 
GACGAGGAAG CAGCCACACC ATCCACCGAG AGCAGGGATG CTGCGGATGA GGACACACCA 
GGGCCAGCGG CGAGGCTGCC AGAGCGAGAT TTGATTTTTG ATGGGGCCAC GCCGGCCACA 
CCGCAGGAGC CCACACGGCA GGAGGACAGT GTCGACCTCC TAGGGCTGCA TGCCGAGGCT 
GGGCTGGCCC CCCCTGTGCA GGCCAGTGGG GCAGCCCCCA GCACCGCCGA CCTGCTCAGC 
CGCCTCCTGG GGGGCCCTGA CGCTGCTCCG GAGGTGCCCC CGGGTGACCT GCTGGGTGGA 
GAGGCTCCCC TCCTCTTCGC AAGCCCAGCC CCCTCCTCGA GCGCACAGAG CCCGCCCCGT 
GAGGGGCCAG CTGCCGACCC CTTTGACCCT CTCCTGCTGC CATCTGGGCC CAACACCCAG 
CCGTGCTCCC AGCCTGACCT GTTCGGTGAA TTTCTTCATT CGGAATCTCC TGCCGCCGTG 
CCTGCGTCCT TCCCGTCCAC CCACAGCGCC CCACCGCCCG CCTGCAGCAC CGACTTCCTG 
CACCTGGGAG ATCTACCAGC AGAGCCTGGC AAGATGACGG CCTCCACCAG CCACCCGGAT 
CTGCTGGGGG GGTGGGAGGC CTGGGCCGAG GCGCCGGCCC CCGCAGCTGC TGCAGAAGGT 
CCCTTCTTCT CTGCAGGAGG CCAGCCTGCC GCCCCCAGTG CCCCCGGGAG CTGGACCAAG 
TCTCAGAGCC TGGACCCGTT TGCTGACCTC GGTGACCTCA GTTCTGGCCT CCAAGGCTCA 
CCGGCTGGAT TTGCTCCAGG GGCTTTTGGC GCCAAGACGG CCCCCCCTCC CACGGGCGGC 
AGCTCCTGGC AGCAGACGGG TCGGCCCCCG GCCCAGGGCA CCCCGTGGCC CCCCCAGGCC 
AAGCCGCCCC CCAAGGCCTG TCCGCAGCCA AGGCCTAACT ATGCTGCCAA CGTGAGCGTG 
ATTGGCGGCC GAGAGGAGAG AGGGGTCCGG GCCCCCAGCT TCGGCCAGAA GCCGAGAGTG 
TCCGAGAGCG ACTTTGAGGA CCTGCTGTCC CACCAGGGCT TCTCGTCCAA GGCTGACCGG 
AAGGGGCCAA GGACCATGGC TGAGATGCGG CGGCAGGAGC AGGCGAGGGA CGCAGACCCG 
CTGAAGCTGA AGCTCCTGGA GTGGACAGAG GGCAAGGAGA GGAACATCCG CGCGCTGCTG 
TCGACCCTGC ACACCGTGCT GTGGGACGGC GAGAGCCGCT GGGCCCCCGT GGGCATGGCC 
GACCTGGTGA CCCCCGGGCA GGTGAAGAAG CAGTACCGCC GCGCCGTGCT GGTGGTGCAC 
CCGGACAAGG CCGCAGGGCA GCCCTACGAG CAGTATGCCA AGATGATCTT CATGGAGCTG 
AACGACGCGT GGGCCGAGTT TGAGAGCCAG GGCTCCCGGC CGCTCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005618746.1
CDS	178..3828
Translation	MKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLTELCKGQLVDFLKKIESRGPLSCDTVLKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKFSIPPNDTRYSVFHDLIRATLKVNPEERLSITELVNQLQEIAAARNVNPKSPITELLEQNGGYGNAVPSRGPPPPGGSSSGYSGGLALAEYDQPYGGLLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRLFLDSKHPGHYAVYNLSPRTYRPSKFHNRVSECGWAARRAPNLRSLYSVCRNMHSWLRQDHKNVCVVHCMDGRAASAVAVCSFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYMCDMVAEEPITPHSKPILVKAVVMTPVPLFSKQRNGCRPFCEVYVGDERVTTTSQEYDKMRDFRIEDGKAVIPLGITVQGDVLIVIYHARSTLGGRLQAKVASMKMFQVQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNVEVEVEPRDRPSREAPPWESTSMRGLNPKILFSSREEQQDILSKFGKPELPRQPGSTAQYDAEAGSLDAEPTESDSPQSSGTDTSHFLHTLDWHEEKDTETSPESHFAKENLSTLAEDAGGSEPSDEEAATPSTESRDAADEDTPGPAARLPERDLIFDGATPATPQEPTRQEDSVDLLGLHAEAGLAPPVQASGAAPSTADLLSRLLGGPDAAPEVPPGDLLGGEAPLLFASPAPSSSAQSPPREGPAADPFDPLLLPSGPNTQPCSQPDLFGEFLHSESPAAVPASFPSTHSAPPPACSTDFLHLGDLPAEPGKMTASTSHPDLLGGWEAWAEAPAPAAAAEGPFFSAGGQPAAPSAPGSWTKSQSLDPFADLGDLSSGLQGSPAGFAPGAFGAKTAPPPTGGSSWQQTGRPPAQGTPWPPQAKPPPKACPQPRPNYAANVSVIGGREERGVRAPSFGQKPRVSESDFEDLLSHQGFSSKADRKGPRTMAEMRRQEQARDADPLKLKLLEWTEGKERNIRALLSTLHTVLWDGESRWAPVGMADLVTPGQVKKQYRRAVLVVHPDKAAGQPYEQYAKMIFMELNDAWAEFESQGSRPLF

Target ORF information:

RefSeq Version	XM_005618689.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005618689.2

ORF Insert Sequence:

ATGAAAAAAC TTTCTGGCCA CCCCAATATT GTCCAGTTTT GTTCTGCAGC GTCCATAGGG 
AAAGAGGAGT CGGACACTGG GCAGGCTGAG TTCCTGCTGC TCACCGAGCT CTGCAAAGGG 
CAGCTGGTGG ACTTTTTAAA GAAAATTGAA TCTAGAGGTC CGCTCTCGTG CGATACTGTT 
CTGAAGATTT TCTATCAGAC GTGCAGAGCG GTGCAGCACA TGCACAGACA AAAGCCGCCC 
ATCATCCATA GAGACCTCAA GGTGGAAAAC TTATTGCTCA GTAACCAAGG GACCATCAAG 
CTGTGTGACT TTGGCAGTGC CACGACCATA TCACATTACC CCGACTACAG CTGGAGCGCC 
CAGAGGCGGG CCCTGGTGGA GGAAGAGATC ACCAGGAACA CCACACCCAT GTACAGGACA 
CCGGAAATCG TGGACTTGTA CTCCAACTTC CCCATCGGCG AGAAGCAGGA CATCTGGGCG 
CTGGGCTGCA TCTTGTATCT GCTGTGCTTC CGGCAGCATC CTTTTGAAGA CGGAGCAAAG 
CTTCGGATAG TCAACGGGAA GTTCTCGATC CCCCCCAATG ACACCCGGTA CTCTGTGTTC 
CACGACCTCA TCCGCGCCAC GCTGAAGGTC AACCCCGAGG AGCGGCTGTC CATCACAGAA 
CTGGTGAACC AGCTCCAGGA GATCGCGGCC GCCAGGAACG TGAACCCCAA GTCTCCCATC 
ACTGAGCTCC TAGAGCAGAA CGGAGGCTAT GGGAACGCTG TCCCTTCCCG AGGGCCACCC 
CCTCCTGGGG GCTCCAGCAG CGGCTACAGC GGAGGCCTGG CGCTGGCCGA GTACGACCAG 
CCCTACGGCG GGCTCCTGGA CATCCTGCGG GGGGGCACGG AGCGCCTCTT CACAAACCTC 
AAGGACACCT CCTCCAAGGT CATCCAGTCT GTTGCCAATT ACGCAAAAGG AGATTTGGAC 
ATCTCTTACA TCACATCTAG AATTGCTGTG ATGTCGTTCC CAGCAGAAGG CGTGGAGTCG 
GCCATCAAGA ACAACATCGA AGACGTGCGG TTGTTTCTGG ACTCCAAGCA CCCGGGACAT 
TACGCCGTCT ACAACCTGTC CCCAAGAACA TACCGGCCCT CCAAATTTCA CAACCGGGTC 
TCCGAGTGTG GCTGGGCGGC CAGACGGGCC CCGAACCTTC GCAGCCTGTA CAGTGTGTGT 
CGGAACATGC ACTCGTGGCT CCGGCAGGAC CACAAGAACG TCTGTGTCGT GCACTGCATG 
GACGGGAGAG CTGCGTCTGC GGTGGCCGTG TGCTCCTTCC TGTGCTTCTG CCGCCTGTTC 
AGCACCGCGG AGGCTGCCGT GTACATGTTC AGCATGAAGC GCTGCCCGCC AGGCATTTGG 
CCATCCCACA AGAGGTACAT CGAGTACATG TGTGACATGG TGGCCGAGGA GCCCATCACA 
CCCCATAGCA AGCCCATCCT GGTGAAAGCC GTGGTCATGA CACCCGTGCC CCTGTTCAGC 
AAGCAGAGAA ACGGCTGCAG GCCCTTCTGC GAGGTCTACG TGGGGGATGA GCGCGTGACT 
ACCACGTCTC AGGAGTACGA CAAGATGAGG GATTTCAGAA TCGAGGATGG CAAAGCAGTC 
ATTCCCCTGG GCATAACAGT GCAAGGGGAC GTGCTCATTG TCATCTACCA CGCCAGGTCC 
ACCCTGGGAG GAAGACTGCA GGCCAAGGTG GCATCCATGA AGATGTTCCA GGTCCAGTTC 
CACACGGGGT TTGTGCCTCG GAATGCGACT ACTGTGAAGT TTGCAAAGTA TGACCTTGAT 
GCGTGTGACA TTCAGGAGAA ATACCCAGAT TTATTCCAAG TGAACGTGGA GGTGGAGGTG 
GAGCCCAGAG ACAGGCCCAG CCGGGAGGCC CCACCCTGGG AGAGCACCAG CATGAGGGGG 
CTCAACCCCA AAATCCTGTT TTCCAGCCGA GAGGAGCAGC AAGACATTCT GTCTAAATTT 
GGGAAGCCGG AGCTCCCCAG GCAGCCCGGC TCCACCGCTC AGTACGACGC CGAGGCAGGG 
TCCCTGGACG CAGAGCCCAC AGAGTCTGAT TCACCACAGA GCAGCGGCAC TGACACCAGC 
CACTTCCTCC ACACGCTGGA TTGGCACGAG GAGAAAGATA CAGAGACGAG TCCAGAAAGT 
CACTTTGCCA AGGAGAACCT ATCAACCCTG GCCGAGGATG CAGGTGGGAG TGAGCCTTCG 
GACGAGGAAG CAGCCACACC ATCCACCGAG AGCAGGGATG CTGCGGATGA GGACACACCA 
GGGCCAGCGG CGAGGCTGCC AGAGCGAGAT TTGATTTTTG ATGGGGCCAC GCCGGCCACA 
CCGCAGGAGC CCACACGGCA GGAGGACAGT GTCGACCTCC TAGGGCTGCA TGCCGAGGCT 
GGGCTGGCCC CCCCTGTGCA GGCCAGTGGG GCAGCCCCCA GCACCGCCGA CCTGCTCAGC 
CGCCTCCTGG GGGGCCCTGA CGCTGCTCCG GAGGTGCCCC CGGGTGACCT GCTGGGTGGA 
GAGGCTCCCC TCCTCTTCGC AAGCCCAGCC CCCTCCTCGA GCGCACAGAG CCCGCCCCGT 
GAGGGGCCAG CTGCCGACCC CTTTGACCCT CTCCTGCTGC CATCTGGGCC CAACACCCAG 
CCGTGCTCCC AGCCTGACCT GTTCGGTGAA TTTCTTCATT CGGAATCTCC TGCCGCCGTG 
CCTGCGTCCT TCCCGTCCAC CCACAGCGCC CCACCGCCCG CCTGCAGCAC CGACTTCCTG 
CACCTGGGAG ATCTACCAGC AGAGCCTGGC AAGATGACGG CCTCCACCAG CCACCCGGAT 
CTGCTGGGGG GGTGGGAGGC CTGGGCCGAG GCGCCGGCCC CCGCAGCTGC TGCAGAAGGT 
CCCTTCTTCT CTGCAGGAGG CCAGCCTGCC GCCCCCAGTG CCCCCGGGAG CTGGACCAAG 
TCTCAGAGCC TGGACCCGTT TGCTGACCTC GGTGACCTCA GTTCTGGCCT CCAAGGCTCA 
CCGGCTGGAT TTGCTCCAGG GGCTTTTGGC GCCAAGACGG CCCCCCCTCC CACGGGCGGC 
AGCTCCTGGC AGCAGACGGG TCGGCCCCCG GCCCAGGGCA CCCCGTGGCC CCCCCAGGCC 
AAGCCGCCCC CCAAGGCCTG TCCGCAGCCA AGGCCTAACT ATGCTGCCAA CGTGAGCGTG 
ATTGGCGGCC GAGAGGAGAG AGGGGTCCGG GCCCCCAGCT TCGGCCAGAA GCCGAGAGTG 
TCCGAGAGCG ACTTTGAGGA CCTGCTGTCC CACCAGGGCT TCTCGTCCAA GGCTGACCGG 
AAGGGGCCAA GGACCATGGC TGAGATGCGG CGGCAGGAGC AGGCGAGGGA CGCAGACCCG 
CTGAAGCTGA AGCTCCTGGA GTGGACAGAG GGCAAGGAGA GGAACATCCG CGCGCTGCTG 
TCGACCCTGC ACACCGTGCT GTGGGACGGC GAGAGCCGCT GGGCCCCCGT GGGCATGGCC 
GACCTGGTGA CCCCCGGGCA GGTGAAGAAG CAGTACCGCC GCGCCGTGCT GGTGGTGCAC 
CCGGACAAGG CCGCAGGGCA GCCCTACGAG CAGTATGCCA AGATGATCTT CATGGAGCTG 
AACGACGCGT GGGCCGAGTT TGAGAGCCAG GGCTCCCGGC CGCTCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAK ( XM_005618687.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005618744.1 Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe04653
Clone ID Related Accession (Same CDS sequence)	XM_005618687.2 , XM_005618689.2 , XM_005618687.3 , XM_022417295.1 , XM_005618689.3 , XM_022417288.1
Accession Version	XM_005618687.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	cyclin-G-associated kinase isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005618687.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAAC TTTCTGGCCA CCCCAATATT GTCCAGTTTT GTTCTGCAGC GTCCATAGGG 
AAAGAGGAGT CGGACACTGG GCAGGCTGAG TTCCTGCTGC TCACCGAGCT CTGCAAAGGG 
CAGCTGGTGG ACTTTTTAAA GAAAATTGAA TCTAGAGGTC CGCTCTCGTG CGATACTGTT 
CTGAAGATTT TCTATCAGAC GTGCAGAGCG GTGCAGCACA TGCACAGACA AAAGCCGCCC 
ATCATCCATA GAGACCTCAA GGTGGAAAAC TTATTGCTCA GTAACCAAGG GACCATCAAG 
CTGTGTGACT TTGGCAGTGC CACGACCATA TCACATTACC CCGACTACAG CTGGAGCGCC 
CAGAGGCGGG CCCTGGTGGA GGAAGAGATC ACCAGGAACA CCACACCCAT GTACAGGACA 
CCGGAAATCG TGGACTTGTA CTCCAACTTC CCCATCGGCG AGAAGCAGGA CATCTGGGCG 
CTGGGCTGCA TCTTGTATCT GCTGTGCTTC CGGCAGCATC CTTTTGAAGA CGGAGCAAAG 
CTTCGGATAG TCAACGGGAA GTTCTCGATC CCCCCCAATG ACACCCGGTA CTCTGTGTTC 
CACGACCTCA TCCGCGCCAC GCTGAAGGTC AACCCCGAGG AGCGGCTGTC CATCACAGAA 
CTGGTGAACC AGCTCCAGGA GATCGCGGCC GCCAGGAACG TGAACCCCAA GTCTCCCATC 
ACTGAGCTCC TAGAGCAGAA CGGAGGCTAT GGGAACGCTG TCCCTTCCCG AGGGCCACCC 
CCTCCTGGGG GCTCCAGCAG CGGCTACAGC GGAGGCCTGG CGCTGGCCGA GTACGACCAG 
CCCTACGGCG GGCTCCTGGA CATCCTGCGG GGGGGCACGG AGCGCCTCTT CACAAACCTC 
AAGGACACCT CCTCCAAGGT CATCCAGTCT GTTGCCAATT ACGCAAAAGG AGATTTGGAC 
ATCTCTTACA TCACATCTAG AATTGCTGTG ATGTCGTTCC CAGCAGAAGG CGTGGAGTCG 
GCCATCAAGA ACAACATCGA AGACGTGCGG TTGTTTCTGG ACTCCAAGCA CCCGGGACAT 
TACGCCGTCT ACAACCTGTC CCCAAGAACA TACCGGCCCT CCAAATTTCA CAACCGGGTC 
TCCGAGTGTG GCTGGGCGGC CAGACGGGCC CCGAACCTTC GCAGCCTGTA CAGTGTGTGT 
CGGAACATGC ACTCGTGGCT CCGGCAGGAC CACAAGAACG TCTGTGTCGT GCACTGCATG 
GACGGGAGAG CTGCGTCTGC GGTGGCCGTG TGCTCCTTCC TGTGCTTCTG CCGCCTGTTC 
AGCACCGCGG AGGCTGCCGT GTACATGTTC AGCATGAAGC GCTGCCCGCC AGGCATTTGG 
CCATCCCACA AGAGGTACAT CGAGTACATG TGTGACATGG TGGCCGAGGA GCCCATCACA 
CCCCATAGCA AGCCCATCCT GGTGAAAGCC GTGGTCATGA CACCCGTGCC CCTGTTCAGC 
AAGCAGAGAA ACGGCTGCAG GCCCTTCTGC GAGGTCTACG TGGGGGATGA GCGCGTGACT 
ACCACGTCTC AGGAGTACGA CAAGATGAGG GATTTCAGAA TCGAGGATGG CAAAGCAGTC 
ATTCCCCTGG GCATAACAGT GCAAGGGGAC GTGCTCATTG TCATCTACCA CGCCAGGTCC 
ACCCTGGGAG GAAGACTGCA GGCCAAGGTG GCATCCATGA AGATGTTCCA GGTCCAGTTC 
CACACGGGGT TTGTGCCTCG GAATGCGACT ACTGTGAAGT TTGCAAAGTA TGACCTTGAT 
GCGTGTGACA TTCAGGAGAA ATACCCAGAT TTATTCCAAG TGAACGTGGA GGTGGAGGTG 
GAGCCCAGAG ACAGGCCCAG CCGGGAGGCC CCACCCTGGG AGAGCACCAG CATGAGGGGG 
CTCAACCCCA AAATCCTGTT TTCCAGCCGA GAGGAGCAGC AAGACATTCT GTCTAAATTT 
GGGAAGCCGG AGCTCCCCAG GCAGCCCGGC TCCACCGCTC AGTACGACGC CGAGGCAGGG 
TCCCTGGACG CAGAGCCCAC AGAGTCTGAT TCACCACAGA GCAGCGGCAC TGACACCAGC 
CACTTCCTCC ACACGCTGGA TTGGCACGAG GAGAAAGATA CAGAGACGAG TCCAGAAAGT 
CACTTTGCCA AGGAGAACCT ATCAACCCTG GCCGAGGATG CAGGTGGGAG TGAGCCTTCG 
GACGAGGAAG CAGCCACACC ATCCACCGAG AGCAGGGATG CTGCGGATGA GGACACACCA 
GGGCCAGCGG CGAGGCTGCC AGAGCGAGAT TTGATTTTTG ATGGGGCCAC GCCGGCCACA 
CCGCAGGAGC CCACACGGCA GGAGGACAGT GTCGACCTCC TAGGGCTGCA TGCCGAGGCT 
GGGCTGGCCC CCCCTGTGCA GGCCAGTGGG GCAGCCCCCA GCACCGCCGA CCTGCTCAGC 
CGCCTCCTGG GGGGCCCTGA CGCTGCTCCG GAGGTGCCCC CGGGTGACCT GCTGGGTGGA 
GAGGCTCCCC TCCTCTTCGC AAGCCCAGCC CCCTCCTCGA GCGCACAGAG CCCGCCCCGT 
GAGGGGCCAG CTGCCGACCC CTTTGACCCT CTCCTGCTGC CATCTGGGCC CAACACCCAG 
CCGTGCTCCC AGCCTGACCT GTTCGGTGAA TTTCTTCATT CGGAATCTCC TGCCGCCGTG 
CCTGCGTCCT TCCCGTCCAC CCACAGCGCC CCACCGCCCG CCTGCAGCAC CGACTTCCTG 
CACCTGGGAG ATCTACCAGC AGAGCCTGGC AAGATGACGG CCTCCACCAG CCACCCGGAT 
CTGCTGGGGG GGTGGGAGGC CTGGGCCGAG GCGCCGGCCC CCGCAGCTGC TGCAGAAGGT 
CCCTTCTTCT CTGCAGGAGG CCAGCCTGCC GCCCCCAGTG CCCCCGGGAG CTGGACCAAG 
TCTCAGAGCC TGGACCCGTT TGCTGACCTC GGTGACCTCA GTTCTGGCCT CCAAGGCTCA 
CCGGCTGGAT TTGCTCCAGG GGCTTTTGGC GCCAAGACGG CCCCCCCTCC CACGGGCGGC 
AGCTCCTGGC AGCAGACGGG TCGGCCCCCG GCCCAGGGCA CCCCGTGGCC CCCCCAGGCC 
AAGCCGCCCC CCAAGGCCTG TCCGCAGCCA AGGCCTAACT ATGCTGCCAA CGTGAGCGTG 
ATTGGCGGCC GAGAGGAGAG AGGGGTCCGG GCCCCCAGCT TCGGCCAGAA GCCGAGAGTG 
TCCGAGAGCG ACTTTGAGGA CCTGCTGTCC CACCAGGGCT TCTCGTCCAA GGCTGACCGG 
AAGGGGCCAA GGACCATGGC TGAGATGCGG CGGCAGGAGC AGGCGAGGGA CGCAGACCCG 
CTGAAGCTGA AGCTCCTGGA GTGGACAGAG GGCAAGGAGA GGAACATCCG CGCGCTGCTG 
TCGACCCTGC ACACCGTGCT GTGGGACGGC GAGAGCCGCT GGGCCCCCGT GGGCATGGCC 
GACCTGGTGA CCCCCGGGCA GGTGAAGAAG CAGTACCGCC GCGCCGTGCT GGTGGTGCAC 
CCGGACAAGG CCGCAGGGCA GCCCTACGAG CAGTATGCCA AGATGATCTT CATGGAGCTG 
AACGACGCGT GGGCCGAGTT TGAGAGCCAG GGCTCCCGGC CGCTCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005618744.1
CDS	229..3879
Translation	MKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLTELCKGQLVDFLKKIESRGPLSCDTVLKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKFSIPPNDTRYSVFHDLIRATLKVNPEERLSITELVNQLQEIAAARNVNPKSPITELLEQNGGYGNAVPSRGPPPPGGSSSGYSGGLALAEYDQPYGGLLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRLFLDSKHPGHYAVYNLSPRTYRPSKFHNRVSECGWAARRAPNLRSLYSVCRNMHSWLRQDHKNVCVVHCMDGRAASAVAVCSFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYMCDMVAEEPITPHSKPILVKAVVMTPVPLFSKQRNGCRPFCEVYVGDERVTTTSQEYDKMRDFRIEDGKAVIPLGITVQGDVLIVIYHARSTLGGRLQAKVASMKMFQVQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNVEVEVEPRDRPSREAPPWESTSMRGLNPKILFSSREEQQDILSKFGKPELPRQPGSTAQYDAEAGSLDAEPTESDSPQSSGTDTSHFLHTLDWHEEKDTETSPESHFAKENLSTLAEDAGGSEPSDEEAATPSTESRDAADEDTPGPAARLPERDLIFDGATPATPQEPTRQEDSVDLLGLHAEAGLAPPVQASGAAPSTADLLSRLLGGPDAAPEVPPGDLLGGEAPLLFASPAPSSSAQSPPREGPAADPFDPLLLPSGPNTQPCSQPDLFGEFLHSESPAAVPASFPSTHSAPPPACSTDFLHLGDLPAEPGKMTASTSHPDLLGGWEAWAEAPAPAAAAEGPFFSAGGQPAAPSAPGSWTKSQSLDPFADLGDLSSGLQGSPAGFAPGAFGAKTAPPPTGGSSWQQTGRPPAQGTPWPPQAKPPPKACPQPRPNYAANVSVIGGREERGVRAPSFGQKPRVSESDFEDLLSHQGFSSKADRKGPRTMAEMRRQEQARDADPLKLKLLEWTEGKERNIRALLSTLHTVLWDGESRWAPVGMADLVTPGQVKKQYRRAVLVVHPDKAAGQPYEQYAKMIFMELNDAWAEFESQGSRPLF

Target ORF information:

RefSeq Version	XM_005618687.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005618687.3

ORF Insert Sequence:

ATGAAAAAAC TTTCTGGCCA CCCCAATATT GTCCAGTTTT GTTCTGCAGC GTCCATAGGG 
AAAGAGGAGT CGGACACTGG GCAGGCTGAG TTCCTGCTGC TCACCGAGCT CTGCAAAGGG 
CAGCTGGTGG ACTTTTTAAA GAAAATTGAA TCTAGAGGTC CGCTCTCGTG CGATACTGTT 
CTGAAGATTT TCTATCAGAC GTGCAGAGCG GTGCAGCACA TGCACAGACA AAAGCCGCCC 
ATCATCCATA GAGACCTCAA GGTGGAAAAC TTATTGCTCA GTAACCAAGG GACCATCAAG 
CTGTGTGACT TTGGCAGTGC CACGACCATA TCACATTACC CCGACTACAG CTGGAGCGCC 
CAGAGGCGGG CCCTGGTGGA GGAAGAGATC ACCAGGAACA CCACACCCAT GTACAGGACA 
CCGGAAATCG TGGACTTGTA CTCCAACTTC CCCATCGGCG AGAAGCAGGA CATCTGGGCG 
CTGGGCTGCA TCTTGTATCT GCTGTGCTTC CGGCAGCATC CTTTTGAAGA CGGAGCAAAG 
CTTCGGATAG TCAACGGGAA GTTCTCGATC CCCCCCAATG ACACCCGGTA CTCTGTGTTC 
CACGACCTCA TCCGCGCCAC GCTGAAGGTC AACCCCGAGG AGCGGCTGTC CATCACAGAA 
CTGGTGAACC AGCTCCAGGA GATCGCGGCC GCCAGGAACG TGAACCCCAA GTCTCCCATC 
ACTGAGCTCC TAGAGCAGAA CGGAGGCTAT GGGAACGCTG TCCCTTCCCG AGGGCCACCC 
CCTCCTGGGG GCTCCAGCAG CGGCTACAGC GGAGGCCTGG CGCTGGCCGA GTACGACCAG 
CCCTACGGCG GGCTCCTGGA CATCCTGCGG GGGGGCACGG AGCGCCTCTT CACAAACCTC 
AAGGACACCT CCTCCAAGGT CATCCAGTCT GTTGCCAATT ACGCAAAAGG AGATTTGGAC 
ATCTCTTACA TCACATCTAG AATTGCTGTG ATGTCGTTCC CAGCAGAAGG CGTGGAGTCG 
GCCATCAAGA ACAACATCGA AGACGTGCGG TTGTTTCTGG ACTCCAAGCA CCCGGGACAT 
TACGCCGTCT ACAACCTGTC CCCAAGAACA TACCGGCCCT CCAAATTTCA CAACCGGGTC 
TCCGAGTGTG GCTGGGCGGC CAGACGGGCC CCGAACCTTC GCAGCCTGTA CAGTGTGTGT 
CGGAACATGC ACTCGTGGCT CCGGCAGGAC CACAAGAACG TCTGTGTCGT GCACTGCATG 
GACGGGAGAG CTGCGTCTGC GGTGGCCGTG TGCTCCTTCC TGTGCTTCTG CCGCCTGTTC 
AGCACCGCGG AGGCTGCCGT GTACATGTTC AGCATGAAGC GCTGCCCGCC AGGCATTTGG 
CCATCCCACA AGAGGTACAT CGAGTACATG TGTGACATGG TGGCCGAGGA GCCCATCACA 
CCCCATAGCA AGCCCATCCT GGTGAAAGCC GTGGTCATGA CACCCGTGCC CCTGTTCAGC 
AAGCAGAGAA ACGGCTGCAG GCCCTTCTGC GAGGTCTACG TGGGGGATGA GCGCGTGACT 
ACCACGTCTC AGGAGTACGA CAAGATGAGG GATTTCAGAA TCGAGGATGG CAAAGCAGTC 
ATTCCCCTGG GCATAACAGT GCAAGGGGAC GTGCTCATTG TCATCTACCA CGCCAGGTCC 
ACCCTGGGAG GAAGACTGCA GGCCAAGGTG GCATCCATGA AGATGTTCCA GGTCCAGTTC 
CACACGGGGT TTGTGCCTCG GAATGCGACT ACTGTGAAGT TTGCAAAGTA TGACCTTGAT 
GCGTGTGACA TTCAGGAGAA ATACCCAGAT TTATTCCAAG TGAACGTGGA GGTGGAGGTG 
GAGCCCAGAG ACAGGCCCAG CCGGGAGGCC CCACCCTGGG AGAGCACCAG CATGAGGGGG 
CTCAACCCCA AAATCCTGTT TTCCAGCCGA GAGGAGCAGC AAGACATTCT GTCTAAATTT 
GGGAAGCCGG AGCTCCCCAG GCAGCCCGGC TCCACCGCTC AGTACGACGC CGAGGCAGGG 
TCCCTGGACG CAGAGCCCAC AGAGTCTGAT TCACCACAGA GCAGCGGCAC TGACACCAGC 
CACTTCCTCC ACACGCTGGA TTGGCACGAG GAGAAAGATA CAGAGACGAG TCCAGAAAGT 
CACTTTGCCA AGGAGAACCT ATCAACCCTG GCCGAGGATG CAGGTGGGAG TGAGCCTTCG 
GACGAGGAAG CAGCCACACC ATCCACCGAG AGCAGGGATG CTGCGGATGA GGACACACCA 
GGGCCAGCGG CGAGGCTGCC AGAGCGAGAT TTGATTTTTG ATGGGGCCAC GCCGGCCACA 
CCGCAGGAGC CCACACGGCA GGAGGACAGT GTCGACCTCC TAGGGCTGCA TGCCGAGGCT 
GGGCTGGCCC CCCCTGTGCA GGCCAGTGGG GCAGCCCCCA GCACCGCCGA CCTGCTCAGC 
CGCCTCCTGG GGGGCCCTGA CGCTGCTCCG GAGGTGCCCC CGGGTGACCT GCTGGGTGGA 
GAGGCTCCCC TCCTCTTCGC AAGCCCAGCC CCCTCCTCGA GCGCACAGAG CCCGCCCCGT 
GAGGGGCCAG CTGCCGACCC CTTTGACCCT CTCCTGCTGC CATCTGGGCC CAACACCCAG 
CCGTGCTCCC AGCCTGACCT GTTCGGTGAA TTTCTTCATT CGGAATCTCC TGCCGCCGTG 
CCTGCGTCCT TCCCGTCCAC CCACAGCGCC CCACCGCCCG CCTGCAGCAC CGACTTCCTG 
CACCTGGGAG ATCTACCAGC AGAGCCTGGC AAGATGACGG CCTCCACCAG CCACCCGGAT 
CTGCTGGGGG GGTGGGAGGC CTGGGCCGAG GCGCCGGCCC CCGCAGCTGC TGCAGAAGGT 
CCCTTCTTCT CTGCAGGAGG CCAGCCTGCC GCCCCCAGTG CCCCCGGGAG CTGGACCAAG 
TCTCAGAGCC TGGACCCGTT TGCTGACCTC GGTGACCTCA GTTCTGGCCT CCAAGGCTCA 
CCGGCTGGAT TTGCTCCAGG GGCTTTTGGC GCCAAGACGG CCCCCCCTCC CACGGGCGGC 
AGCTCCTGGC AGCAGACGGG TCGGCCCCCG GCCCAGGGCA CCCCGTGGCC CCCCCAGGCC 
AAGCCGCCCC CCAAGGCCTG TCCGCAGCCA AGGCCTAACT ATGCTGCCAA CGTGAGCGTG 
ATTGGCGGCC GAGAGGAGAG AGGGGTCCGG GCCCCCAGCT TCGGCCAGAA GCCGAGAGTG 
TCCGAGAGCG ACTTTGAGGA CCTGCTGTCC CACCAGGGCT TCTCGTCCAA GGCTGACCGG 
AAGGGGCCAA GGACCATGGC TGAGATGCGG CGGCAGGAGC AGGCGAGGGA CGCAGACCCG 
CTGAAGCTGA AGCTCCTGGA GTGGACAGAG GGCAAGGAGA GGAACATCCG CGCGCTGCTG 
TCGACCCTGC ACACCGTGCT GTGGGACGGC GAGAGCCGCT GGGCCCCCGT GGGCATGGCC 
GACCTGGTGA CCCCCGGGCA GGTGAAGAAG CAGTACCGCC GCGCCGTGCT GGTGGTGCAC 
CCGGACAAGG CCGCAGGGCA GCCCTACGAG CAGTATGCCA AGATGATCTT CATGGAGCTG 
AACGACGCGT GGGCCGAGTT TGAGAGCCAG GGCTCCCGGC CGCTCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAK ( XM_022417295.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022273003.1 Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X15, mRNA.

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CloneID	OCe04653
Clone ID Related Accession (Same CDS sequence)	XM_005618687.2 , XM_005618689.2 , XM_005618687.3 , XM_022417295.1 , XM_005618689.3 , XM_022417288.1
Accession Version	XM_022417295.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	cyclin-G-associated kinase isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAAC TTTCTGGCCA CCCCAATATT GTCCAGTTTT GTTCTGCAGC GTCCATAGGG 
AAAGAGGAGT CGGACACTGG GCAGGCTGAG TTCCTGCTGC TCACCGAGCT CTGCAAAGGG 
CAGCTGGTGG ACTTTTTAAA GAAAATTGAA TCTAGAGGTC CGCTCTCGTG CGATACTGTT 
CTGAAGATTT TCTATCAGAC GTGCAGAGCG GTGCAGCACA TGCACAGACA AAAGCCGCCC 
ATCATCCATA GAGACCTCAA GGTGGAAAAC TTATTGCTCA GTAACCAAGG GACCATCAAG 
CTGTGTGACT TTGGCAGTGC CACGACCATA TCACATTACC CCGACTACAG CTGGAGCGCC 
CAGAGGCGGG CCCTGGTGGA GGAAGAGATC ACCAGGAACA CCACACCCAT GTACAGGACA 
CCGGAAATCG TGGACTTGTA CTCCAACTTC CCCATCGGCG AGAAGCAGGA CATCTGGGCG 
CTGGGCTGCA TCTTGTATCT GCTGTGCTTC CGGCAGCATC CTTTTGAAGA CGGAGCAAAG 
CTTCGGATAG TCAACGGGAA GTTCTCGATC CCCCCCAATG ACACCCGGTA CTCTGTGTTC 
CACGACCTCA TCCGCGCCAC GCTGAAGGTC AACCCCGAGG AGCGGCTGTC CATCACAGAA 
CTGGTGAACC AGCTCCAGGA GATCGCGGCC GCCAGGAACG TGAACCCCAA GTCTCCCATC 
ACTGAGCTCC TAGAGCAGAA CGGAGGCTAT GGGAACGCTG TCCCTTCCCG AGGGCCACCC 
CCTCCTGGGG GCTCCAGCAG CGGCTACAGC GGAGGCCTGG CGCTGGCCGA GTACGACCAG 
CCCTACGGCG GGCTCCTGGA CATCCTGCGG GGGGGCACGG AGCGCCTCTT CACAAACCTC 
AAGGACACCT CCTCCAAGGT CATCCAGTCT GTTGCCAATT ACGCAAAAGG AGATTTGGAC 
ATCTCTTACA TCACATCTAG AATTGCTGTG ATGTCGTTCC CAGCAGAAGG CGTGGAGTCG 
GCCATCAAGA ACAACATCGA AGACGTGCGG TTGTTTCTGG ACTCCAAGCA CCCGGGACAT 
TACGCCGTCT ACAACCTGTC CCCAAGAACA TACCGGCCCT CCAAATTTCA CAACCGGGTC 
TCCGAGTGTG GCTGGGCGGC CAGACGGGCC CCGAACCTTC GCAGCCTGTA CAGTGTGTGT 
CGGAACATGC ACTCGTGGCT CCGGCAGGAC CACAAGAACG TCTGTGTCGT GCACTGCATG 
GACGGGAGAG CTGCGTCTGC GGTGGCCGTG TGCTCCTTCC TGTGCTTCTG CCGCCTGTTC 
AGCACCGCGG AGGCTGCCGT GTACATGTTC AGCATGAAGC GCTGCCCGCC AGGCATTTGG 
CCATCCCACA AGAGGTACAT CGAGTACATG TGTGACATGG TGGCCGAGGA GCCCATCACA 
CCCCATAGCA AGCCCATCCT GGTGAAAGCC GTGGTCATGA CACCCGTGCC CCTGTTCAGC 
AAGCAGAGAA ACGGCTGCAG GCCCTTCTGC GAGGTCTACG TGGGGGATGA GCGCGTGACT 
ACCACGTCTC AGGAGTACGA CAAGATGAGG GATTTCAGAA TCGAGGATGG CAAAGCAGTC 
ATTCCCCTGG GCATAACAGT GCAAGGGGAC GTGCTCATTG TCATCTACCA CGCCAGGTCC 
ACCCTGGGAG GAAGACTGCA GGCCAAGGTG GCATCCATGA AGATGTTCCA GGTCCAGTTC 
CACACGGGGT TTGTGCCTCG GAATGCGACT ACTGTGAAGT TTGCAAAGTA TGACCTTGAT 
GCGTGTGACA TTCAGGAGAA ATACCCAGAT TTATTCCAAG TGAACGTGGA GGTGGAGGTG 
GAGCCCAGAG ACAGGCCCAG CCGGGAGGCC CCACCCTGGG AGAGCACCAG CATGAGGGGG 
CTCAACCCCA AAATCCTGTT TTCCAGCCGA GAGGAGCAGC AAGACATTCT GTCTAAATTT 
GGGAAGCCGG AGCTCCCCAG GCAGCCCGGC TCCACCGCTC AGTACGACGC CGAGGCAGGG 
TCCCTGGACG CAGAGCCCAC AGAGTCTGAT TCACCACAGA GCAGCGGCAC TGACACCAGC 
CACTTCCTCC ACACGCTGGA TTGGCACGAG GAGAAAGATA CAGAGACGAG TCCAGAAAGT 
CACTTTGCCA AGGAGAACCT ATCAACCCTG GCCGAGGATG CAGGTGGGAG TGAGCCTTCG 
GACGAGGAAG CAGCCACACC ATCCACCGAG AGCAGGGATG CTGCGGATGA GGACACACCA 
GGGCCAGCGG CGAGGCTGCC AGAGCGAGAT TTGATTTTTG ATGGGGCCAC GCCGGCCACA 
CCGCAGGAGC CCACACGGCA GGAGGACAGT GTCGACCTCC TAGGGCTGCA TGCCGAGGCT 
GGGCTGGCCC CCCCTGTGCA GGCCAGTGGG GCAGCCCCCA GCACCGCCGA CCTGCTCAGC 
CGCCTCCTGG GGGGCCCTGA CGCTGCTCCG GAGGTGCCCC CGGGTGACCT GCTGGGTGGA 
GAGGCTCCCC TCCTCTTCGC AAGCCCAGCC CCCTCCTCGA GCGCACAGAG CCCGCCCCGT 
GAGGGGCCAG CTGCCGACCC CTTTGACCCT CTCCTGCTGC CATCTGGGCC CAACACCCAG 
CCGTGCTCCC AGCCTGACCT GTTCGGTGAA TTTCTTCATT CGGAATCTCC TGCCGCCGTG 
CCTGCGTCCT TCCCGTCCAC CCACAGCGCC CCACCGCCCG CCTGCAGCAC CGACTTCCTG 
CACCTGGGAG ATCTACCAGC AGAGCCTGGC AAGATGACGG CCTCCACCAG CCACCCGGAT 
CTGCTGGGGG GGTGGGAGGC CTGGGCCGAG GCGCCGGCCC CCGCAGCTGC TGCAGAAGGT 
CCCTTCTTCT CTGCAGGAGG CCAGCCTGCC GCCCCCAGTG CCCCCGGGAG CTGGACCAAG 
TCTCAGAGCC TGGACCCGTT TGCTGACCTC GGTGACCTCA GTTCTGGCCT CCAAGGCTCA 
CCGGCTGGAT TTGCTCCAGG GGCTTTTGGC GCCAAGACGG CCCCCCCTCC CACGGGCGGC 
AGCTCCTGGC AGCAGACGGG TCGGCCCCCG GCCCAGGGCA CCCCGTGGCC CCCCCAGGCC 
AAGCCGCCCC CCAAGGCCTG TCCGCAGCCA AGGCCTAACT ATGCTGCCAA CGTGAGCGTG 
ATTGGCGGCC GAGAGGAGAG AGGGGTCCGG GCCCCCAGCT TCGGCCAGAA GCCGAGAGTG 
TCCGAGAGCG ACTTTGAGGA CCTGCTGTCC CACCAGGGCT TCTCGTCCAA GGCTGACCGG 
AAGGGGCCAA GGACCATGGC TGAGATGCGG CGGCAGGAGC AGGCGAGGGA CGCAGACCCG 
CTGAAGCTGA AGCTCCTGGA GTGGACAGAG GGCAAGGAGA GGAACATCCG CGCGCTGCTG 
TCGACCCTGC ACACCGTGCT GTGGGACGGC GAGAGCCGCT GGGCCCCCGT GGGCATGGCC 
GACCTGGTGA CCCCCGGGCA GGTGAAGAAG CAGTACCGCC GCGCCGTGCT GGTGGTGCAC 
CCGGACAAGG CCGCAGGGCA GCCCTACGAG CAGTATGCCA AGATGATCTT CATGGAGCTG 
AACGACGCGT GGGCCGAGTT TGAGAGCCAG GGCTCCCGGC CGCTCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022273003.1
CDS	187..3837
Translation	MKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLTELCKGQLVDFLKKIESRGPLSCDTVLKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKFSIPPNDTRYSVFHDLIRATLKVNPEERLSITELVNQLQEIAAARNVNPKSPITELLEQNGGYGNAVPSRGPPPPGGSSSGYSGGLALAEYDQPYGGLLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRLFLDSKHPGHYAVYNLSPRTYRPSKFHNRVSECGWAARRAPNLRSLYSVCRNMHSWLRQDHKNVCVVHCMDGRAASAVAVCSFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYMCDMVAEEPITPHSKPILVKAVVMTPVPLFSKQRNGCRPFCEVYVGDERVTTTSQEYDKMRDFRIEDGKAVIPLGITVQGDVLIVIYHARSTLGGRLQAKVASMKMFQVQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNVEVEVEPRDRPSREAPPWESTSMRGLNPKILFSSREEQQDILSKFGKPELPRQPGSTAQYDAEAGSLDAEPTESDSPQSSGTDTSHFLHTLDWHEEKDTETSPESHFAKENLSTLAEDAGGSEPSDEEAATPSTESRDAADEDTPGPAARLPERDLIFDGATPATPQEPTRQEDSVDLLGLHAEAGLAPPVQASGAAPSTADLLSRLLGGPDAAPEVPPGDLLGGEAPLLFASPAPSSSAQSPPREGPAADPFDPLLLPSGPNTQPCSQPDLFGEFLHSESPAAVPASFPSTHSAPPPACSTDFLHLGDLPAEPGKMTASTSHPDLLGGWEAWAEAPAPAAAAEGPFFSAGGQPAAPSAPGSWTKSQSLDPFADLGDLSSGLQGSPAGFAPGAFGAKTAPPPTGGSSWQQTGRPPAQGTPWPPQAKPPPKACPQPRPNYAANVSVIGGREERGVRAPSFGQKPRVSESDFEDLLSHQGFSSKADRKGPRTMAEMRRQEQARDADPLKLKLLEWTEGKERNIRALLSTLHTVLWDGESRWAPVGMADLVTPGQVKKQYRRAVLVVHPDKAAGQPYEQYAKMIFMELNDAWAEFESQGSRPLF

Target ORF information:

RefSeq Version	XM_022417295.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022417295.1

ORF Insert Sequence:

ATGAAAAAAC TTTCTGGCCA CCCCAATATT GTCCAGTTTT GTTCTGCAGC GTCCATAGGG 
AAAGAGGAGT CGGACACTGG GCAGGCTGAG TTCCTGCTGC TCACCGAGCT CTGCAAAGGG 
CAGCTGGTGG ACTTTTTAAA GAAAATTGAA TCTAGAGGTC CGCTCTCGTG CGATACTGTT 
CTGAAGATTT TCTATCAGAC GTGCAGAGCG GTGCAGCACA TGCACAGACA AAAGCCGCCC 
ATCATCCATA GAGACCTCAA GGTGGAAAAC TTATTGCTCA GTAACCAAGG GACCATCAAG 
CTGTGTGACT TTGGCAGTGC CACGACCATA TCACATTACC CCGACTACAG CTGGAGCGCC 
CAGAGGCGGG CCCTGGTGGA GGAAGAGATC ACCAGGAACA CCACACCCAT GTACAGGACA 
CCGGAAATCG TGGACTTGTA CTCCAACTTC CCCATCGGCG AGAAGCAGGA CATCTGGGCG 
CTGGGCTGCA TCTTGTATCT GCTGTGCTTC CGGCAGCATC CTTTTGAAGA CGGAGCAAAG 
CTTCGGATAG TCAACGGGAA GTTCTCGATC CCCCCCAATG ACACCCGGTA CTCTGTGTTC 
CACGACCTCA TCCGCGCCAC GCTGAAGGTC AACCCCGAGG AGCGGCTGTC CATCACAGAA 
CTGGTGAACC AGCTCCAGGA GATCGCGGCC GCCAGGAACG TGAACCCCAA GTCTCCCATC 
ACTGAGCTCC TAGAGCAGAA CGGAGGCTAT GGGAACGCTG TCCCTTCCCG AGGGCCACCC 
CCTCCTGGGG GCTCCAGCAG CGGCTACAGC GGAGGCCTGG CGCTGGCCGA GTACGACCAG 
CCCTACGGCG GGCTCCTGGA CATCCTGCGG GGGGGCACGG AGCGCCTCTT CACAAACCTC 
AAGGACACCT CCTCCAAGGT CATCCAGTCT GTTGCCAATT ACGCAAAAGG AGATTTGGAC 
ATCTCTTACA TCACATCTAG AATTGCTGTG ATGTCGTTCC CAGCAGAAGG CGTGGAGTCG 
GCCATCAAGA ACAACATCGA AGACGTGCGG TTGTTTCTGG ACTCCAAGCA CCCGGGACAT 
TACGCCGTCT ACAACCTGTC CCCAAGAACA TACCGGCCCT CCAAATTTCA CAACCGGGTC 
TCCGAGTGTG GCTGGGCGGC CAGACGGGCC CCGAACCTTC GCAGCCTGTA CAGTGTGTGT 
CGGAACATGC ACTCGTGGCT CCGGCAGGAC CACAAGAACG TCTGTGTCGT GCACTGCATG 
GACGGGAGAG CTGCGTCTGC GGTGGCCGTG TGCTCCTTCC TGTGCTTCTG CCGCCTGTTC 
AGCACCGCGG AGGCTGCCGT GTACATGTTC AGCATGAAGC GCTGCCCGCC AGGCATTTGG 
CCATCCCACA AGAGGTACAT CGAGTACATG TGTGACATGG TGGCCGAGGA GCCCATCACA 
CCCCATAGCA AGCCCATCCT GGTGAAAGCC GTGGTCATGA CACCCGTGCC CCTGTTCAGC 
AAGCAGAGAA ACGGCTGCAG GCCCTTCTGC GAGGTCTACG TGGGGGATGA GCGCGTGACT 
ACCACGTCTC AGGAGTACGA CAAGATGAGG GATTTCAGAA TCGAGGATGG CAAAGCAGTC 
ATTCCCCTGG GCATAACAGT GCAAGGGGAC GTGCTCATTG TCATCTACCA CGCCAGGTCC 
ACCCTGGGAG GAAGACTGCA GGCCAAGGTG GCATCCATGA AGATGTTCCA GGTCCAGTTC 
CACACGGGGT TTGTGCCTCG GAATGCGACT ACTGTGAAGT TTGCAAAGTA TGACCTTGAT 
GCGTGTGACA TTCAGGAGAA ATACCCAGAT TTATTCCAAG TGAACGTGGA GGTGGAGGTG 
GAGCCCAGAG ACAGGCCCAG CCGGGAGGCC CCACCCTGGG AGAGCACCAG CATGAGGGGG 
CTCAACCCCA AAATCCTGTT TTCCAGCCGA GAGGAGCAGC AAGACATTCT GTCTAAATTT 
GGGAAGCCGG AGCTCCCCAG GCAGCCCGGC TCCACCGCTC AGTACGACGC CGAGGCAGGG 
TCCCTGGACG CAGAGCCCAC AGAGTCTGAT TCACCACAGA GCAGCGGCAC TGACACCAGC 
CACTTCCTCC ACACGCTGGA TTGGCACGAG GAGAAAGATA CAGAGACGAG TCCAGAAAGT 
CACTTTGCCA AGGAGAACCT ATCAACCCTG GCCGAGGATG CAGGTGGGAG TGAGCCTTCG 
GACGAGGAAG CAGCCACACC ATCCACCGAG AGCAGGGATG CTGCGGATGA GGACACACCA 
GGGCCAGCGG CGAGGCTGCC AGAGCGAGAT TTGATTTTTG ATGGGGCCAC GCCGGCCACA 
CCGCAGGAGC CCACACGGCA GGAGGACAGT GTCGACCTCC TAGGGCTGCA TGCCGAGGCT 
GGGCTGGCCC CCCCTGTGCA GGCCAGTGGG GCAGCCCCCA GCACCGCCGA CCTGCTCAGC 
CGCCTCCTGG GGGGCCCTGA CGCTGCTCCG GAGGTGCCCC CGGGTGACCT GCTGGGTGGA 
GAGGCTCCCC TCCTCTTCGC AAGCCCAGCC CCCTCCTCGA GCGCACAGAG CCCGCCCCGT 
GAGGGGCCAG CTGCCGACCC CTTTGACCCT CTCCTGCTGC CATCTGGGCC CAACACCCAG 
CCGTGCTCCC AGCCTGACCT GTTCGGTGAA TTTCTTCATT CGGAATCTCC TGCCGCCGTG 
CCTGCGTCCT TCCCGTCCAC CCACAGCGCC CCACCGCCCG CCTGCAGCAC CGACTTCCTG 
CACCTGGGAG ATCTACCAGC AGAGCCTGGC AAGATGACGG CCTCCACCAG CCACCCGGAT 
CTGCTGGGGG GGTGGGAGGC CTGGGCCGAG GCGCCGGCCC CCGCAGCTGC TGCAGAAGGT 
CCCTTCTTCT CTGCAGGAGG CCAGCCTGCC GCCCCCAGTG CCCCCGGGAG CTGGACCAAG 
TCTCAGAGCC TGGACCCGTT TGCTGACCTC GGTGACCTCA GTTCTGGCCT CCAAGGCTCA 
CCGGCTGGAT TTGCTCCAGG GGCTTTTGGC GCCAAGACGG CCCCCCCTCC CACGGGCGGC 
AGCTCCTGGC AGCAGACGGG TCGGCCCCCG GCCCAGGGCA CCCCGTGGCC CCCCCAGGCC 
AAGCCGCCCC CCAAGGCCTG TCCGCAGCCA AGGCCTAACT ATGCTGCCAA CGTGAGCGTG 
ATTGGCGGCC GAGAGGAGAG AGGGGTCCGG GCCCCCAGCT TCGGCCAGAA GCCGAGAGTG 
TCCGAGAGCG ACTTTGAGGA CCTGCTGTCC CACCAGGGCT TCTCGTCCAA GGCTGACCGG 
AAGGGGCCAA GGACCATGGC TGAGATGCGG CGGCAGGAGC AGGCGAGGGA CGCAGACCCG 
CTGAAGCTGA AGCTCCTGGA GTGGACAGAG GGCAAGGAGA GGAACATCCG CGCGCTGCTG 
TCGACCCTGC ACACCGTGCT GTGGGACGGC GAGAGCCGCT GGGCCCCCGT GGGCATGGCC 
GACCTGGTGA CCCCCGGGCA GGTGAAGAAG CAGTACCGCC GCGCCGTGCT GGTGGTGCAC 
CCGGACAAGG CCGCAGGGCA GCCCTACGAG CAGTATGCCA AGATGATCTT CATGGAGCTG 
AACGACGCGT GGGCCGAGTT TGAGAGCCAG GGCTCCCGGC CGCTCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAK ( XM_005618689.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005618746.1 Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OCe04653
Clone ID Related Accession (Same CDS sequence)	XM_005618687.2 , XM_005618689.2 , XM_005618687.3 , XM_022417295.1 , XM_005618689.3 , XM_022417288.1
Accession Version	XM_005618689.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	cyclin-G-associated kinase isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005618689.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAAC TTTCTGGCCA CCCCAATATT GTCCAGTTTT GTTCTGCAGC GTCCATAGGG 
AAAGAGGAGT CGGACACTGG GCAGGCTGAG TTCCTGCTGC TCACCGAGCT CTGCAAAGGG 
CAGCTGGTGG ACTTTTTAAA GAAAATTGAA TCTAGAGGTC CGCTCTCGTG CGATACTGTT 
CTGAAGATTT TCTATCAGAC GTGCAGAGCG GTGCAGCACA TGCACAGACA AAAGCCGCCC 
ATCATCCATA GAGACCTCAA GGTGGAAAAC TTATTGCTCA GTAACCAAGG GACCATCAAG 
CTGTGTGACT TTGGCAGTGC CACGACCATA TCACATTACC CCGACTACAG CTGGAGCGCC 
CAGAGGCGGG CCCTGGTGGA GGAAGAGATC ACCAGGAACA CCACACCCAT GTACAGGACA 
CCGGAAATCG TGGACTTGTA CTCCAACTTC CCCATCGGCG AGAAGCAGGA CATCTGGGCG 
CTGGGCTGCA TCTTGTATCT GCTGTGCTTC CGGCAGCATC CTTTTGAAGA CGGAGCAAAG 
CTTCGGATAG TCAACGGGAA GTTCTCGATC CCCCCCAATG ACACCCGGTA CTCTGTGTTC 
CACGACCTCA TCCGCGCCAC GCTGAAGGTC AACCCCGAGG AGCGGCTGTC CATCACAGAA 
CTGGTGAACC AGCTCCAGGA GATCGCGGCC GCCAGGAACG TGAACCCCAA GTCTCCCATC 
ACTGAGCTCC TAGAGCAGAA CGGAGGCTAT GGGAACGCTG TCCCTTCCCG AGGGCCACCC 
CCTCCTGGGG GCTCCAGCAG CGGCTACAGC GGAGGCCTGG CGCTGGCCGA GTACGACCAG 
CCCTACGGCG GGCTCCTGGA CATCCTGCGG GGGGGCACGG AGCGCCTCTT CACAAACCTC 
AAGGACACCT CCTCCAAGGT CATCCAGTCT GTTGCCAATT ACGCAAAAGG AGATTTGGAC 
ATCTCTTACA TCACATCTAG AATTGCTGTG ATGTCGTTCC CAGCAGAAGG CGTGGAGTCG 
GCCATCAAGA ACAACATCGA AGACGTGCGG TTGTTTCTGG ACTCCAAGCA CCCGGGACAT 
TACGCCGTCT ACAACCTGTC CCCAAGAACA TACCGGCCCT CCAAATTTCA CAACCGGGTC 
TCCGAGTGTG GCTGGGCGGC CAGACGGGCC CCGAACCTTC GCAGCCTGTA CAGTGTGTGT 
CGGAACATGC ACTCGTGGCT CCGGCAGGAC CACAAGAACG TCTGTGTCGT GCACTGCATG 
GACGGGAGAG CTGCGTCTGC GGTGGCCGTG TGCTCCTTCC TGTGCTTCTG CCGCCTGTTC 
AGCACCGCGG AGGCTGCCGT GTACATGTTC AGCATGAAGC GCTGCCCGCC AGGCATTTGG 
CCATCCCACA AGAGGTACAT CGAGTACATG TGTGACATGG TGGCCGAGGA GCCCATCACA 
CCCCATAGCA AGCCCATCCT GGTGAAAGCC GTGGTCATGA CACCCGTGCC CCTGTTCAGC 
AAGCAGAGAA ACGGCTGCAG GCCCTTCTGC GAGGTCTACG TGGGGGATGA GCGCGTGACT 
ACCACGTCTC AGGAGTACGA CAAGATGAGG GATTTCAGAA TCGAGGATGG CAAAGCAGTC 
ATTCCCCTGG GCATAACAGT GCAAGGGGAC GTGCTCATTG TCATCTACCA CGCCAGGTCC 
ACCCTGGGAG GAAGACTGCA GGCCAAGGTG GCATCCATGA AGATGTTCCA GGTCCAGTTC 
CACACGGGGT TTGTGCCTCG GAATGCGACT ACTGTGAAGT TTGCAAAGTA TGACCTTGAT 
GCGTGTGACA TTCAGGAGAA ATACCCAGAT TTATTCCAAG TGAACGTGGA GGTGGAGGTG 
GAGCCCAGAG ACAGGCCCAG CCGGGAGGCC CCACCCTGGG AGAGCACCAG CATGAGGGGG 
CTCAACCCCA AAATCCTGTT TTCCAGCCGA GAGGAGCAGC AAGACATTCT GTCTAAATTT 
GGGAAGCCGG AGCTCCCCAG GCAGCCCGGC TCCACCGCTC AGTACGACGC CGAGGCAGGG 
TCCCTGGACG CAGAGCCCAC AGAGTCTGAT TCACCACAGA GCAGCGGCAC TGACACCAGC 
CACTTCCTCC ACACGCTGGA TTGGCACGAG GAGAAAGATA CAGAGACGAG TCCAGAAAGT 
CACTTTGCCA AGGAGAACCT ATCAACCCTG GCCGAGGATG CAGGTGGGAG TGAGCCTTCG 
GACGAGGAAG CAGCCACACC ATCCACCGAG AGCAGGGATG CTGCGGATGA GGACACACCA 
GGGCCAGCGG CGAGGCTGCC AGAGCGAGAT TTGATTTTTG ATGGGGCCAC GCCGGCCACA 
CCGCAGGAGC CCACACGGCA GGAGGACAGT GTCGACCTCC TAGGGCTGCA TGCCGAGGCT 
GGGCTGGCCC CCCCTGTGCA GGCCAGTGGG GCAGCCCCCA GCACCGCCGA CCTGCTCAGC 
CGCCTCCTGG GGGGCCCTGA CGCTGCTCCG GAGGTGCCCC CGGGTGACCT GCTGGGTGGA 
GAGGCTCCCC TCCTCTTCGC AAGCCCAGCC CCCTCCTCGA GCGCACAGAG CCCGCCCCGT 
GAGGGGCCAG CTGCCGACCC CTTTGACCCT CTCCTGCTGC CATCTGGGCC CAACACCCAG 
CCGTGCTCCC AGCCTGACCT GTTCGGTGAA TTTCTTCATT CGGAATCTCC TGCCGCCGTG 
CCTGCGTCCT TCCCGTCCAC CCACAGCGCC CCACCGCCCG CCTGCAGCAC CGACTTCCTG 
CACCTGGGAG ATCTACCAGC AGAGCCTGGC AAGATGACGG CCTCCACCAG CCACCCGGAT 
CTGCTGGGGG GGTGGGAGGC CTGGGCCGAG GCGCCGGCCC CCGCAGCTGC TGCAGAAGGT 
CCCTTCTTCT CTGCAGGAGG CCAGCCTGCC GCCCCCAGTG CCCCCGGGAG CTGGACCAAG 
TCTCAGAGCC TGGACCCGTT TGCTGACCTC GGTGACCTCA GTTCTGGCCT CCAAGGCTCA 
CCGGCTGGAT TTGCTCCAGG GGCTTTTGGC GCCAAGACGG CCCCCCCTCC CACGGGCGGC 
AGCTCCTGGC AGCAGACGGG TCGGCCCCCG GCCCAGGGCA CCCCGTGGCC CCCCCAGGCC 
AAGCCGCCCC CCAAGGCCTG TCCGCAGCCA AGGCCTAACT ATGCTGCCAA CGTGAGCGTG 
ATTGGCGGCC GAGAGGAGAG AGGGGTCCGG GCCCCCAGCT TCGGCCAGAA GCCGAGAGTG 
TCCGAGAGCG ACTTTGAGGA CCTGCTGTCC CACCAGGGCT TCTCGTCCAA GGCTGACCGG 
AAGGGGCCAA GGACCATGGC TGAGATGCGG CGGCAGGAGC AGGCGAGGGA CGCAGACCCG 
CTGAAGCTGA AGCTCCTGGA GTGGACAGAG GGCAAGGAGA GGAACATCCG CGCGCTGCTG 
TCGACCCTGC ACACCGTGCT GTGGGACGGC GAGAGCCGCT GGGCCCCCGT GGGCATGGCC 
GACCTGGTGA CCCCCGGGCA GGTGAAGAAG CAGTACCGCC GCGCCGTGCT GGTGGTGCAC 
CCGGACAAGG CCGCAGGGCA GCCCTACGAG CAGTATGCCA AGATGATCTT CATGGAGCTG 
AACGACGCGT GGGCCGAGTT TGAGAGCCAG GGCTCCCGGC CGCTCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005618746.1
CDS	233..3883
Translation	MKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLTELCKGQLVDFLKKIESRGPLSCDTVLKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKFSIPPNDTRYSVFHDLIRATLKVNPEERLSITELVNQLQEIAAARNVNPKSPITELLEQNGGYGNAVPSRGPPPPGGSSSGYSGGLALAEYDQPYGGLLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRLFLDSKHPGHYAVYNLSPRTYRPSKFHNRVSECGWAARRAPNLRSLYSVCRNMHSWLRQDHKNVCVVHCMDGRAASAVAVCSFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYMCDMVAEEPITPHSKPILVKAVVMTPVPLFSKQRNGCRPFCEVYVGDERVTTTSQEYDKMRDFRIEDGKAVIPLGITVQGDVLIVIYHARSTLGGRLQAKVASMKMFQVQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNVEVEVEPRDRPSREAPPWESTSMRGLNPKILFSSREEQQDILSKFGKPELPRQPGSTAQYDAEAGSLDAEPTESDSPQSSGTDTSHFLHTLDWHEEKDTETSPESHFAKENLSTLAEDAGGSEPSDEEAATPSTESRDAADEDTPGPAARLPERDLIFDGATPATPQEPTRQEDSVDLLGLHAEAGLAPPVQASGAAPSTADLLSRLLGGPDAAPEVPPGDLLGGEAPLLFASPAPSSSAQSPPREGPAADPFDPLLLPSGPNTQPCSQPDLFGEFLHSESPAAVPASFPSTHSAPPPACSTDFLHLGDLPAEPGKMTASTSHPDLLGGWEAWAEAPAPAAAAEGPFFSAGGQPAAPSAPGSWTKSQSLDPFADLGDLSSGLQGSPAGFAPGAFGAKTAPPPTGGSSWQQTGRPPAQGTPWPPQAKPPPKACPQPRPNYAANVSVIGGREERGVRAPSFGQKPRVSESDFEDLLSHQGFSSKADRKGPRTMAEMRRQEQARDADPLKLKLLEWTEGKERNIRALLSTLHTVLWDGESRWAPVGMADLVTPGQVKKQYRRAVLVVHPDKAAGQPYEQYAKMIFMELNDAWAEFESQGSRPLF

Target ORF information:

RefSeq Version	XM_005618689.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005618689.3

ORF Insert Sequence:

ATGAAAAAAC TTTCTGGCCA CCCCAATATT GTCCAGTTTT GTTCTGCAGC GTCCATAGGG 
AAAGAGGAGT CGGACACTGG GCAGGCTGAG TTCCTGCTGC TCACCGAGCT CTGCAAAGGG 
CAGCTGGTGG ACTTTTTAAA GAAAATTGAA TCTAGAGGTC CGCTCTCGTG CGATACTGTT 
CTGAAGATTT TCTATCAGAC GTGCAGAGCG GTGCAGCACA TGCACAGACA AAAGCCGCCC 
ATCATCCATA GAGACCTCAA GGTGGAAAAC TTATTGCTCA GTAACCAAGG GACCATCAAG 
CTGTGTGACT TTGGCAGTGC CACGACCATA TCACATTACC CCGACTACAG CTGGAGCGCC 
CAGAGGCGGG CCCTGGTGGA GGAAGAGATC ACCAGGAACA CCACACCCAT GTACAGGACA 
CCGGAAATCG TGGACTTGTA CTCCAACTTC CCCATCGGCG AGAAGCAGGA CATCTGGGCG 
CTGGGCTGCA TCTTGTATCT GCTGTGCTTC CGGCAGCATC CTTTTGAAGA CGGAGCAAAG 
CTTCGGATAG TCAACGGGAA GTTCTCGATC CCCCCCAATG ACACCCGGTA CTCTGTGTTC 
CACGACCTCA TCCGCGCCAC GCTGAAGGTC AACCCCGAGG AGCGGCTGTC CATCACAGAA 
CTGGTGAACC AGCTCCAGGA GATCGCGGCC GCCAGGAACG TGAACCCCAA GTCTCCCATC 
ACTGAGCTCC TAGAGCAGAA CGGAGGCTAT GGGAACGCTG TCCCTTCCCG AGGGCCACCC 
CCTCCTGGGG GCTCCAGCAG CGGCTACAGC GGAGGCCTGG CGCTGGCCGA GTACGACCAG 
CCCTACGGCG GGCTCCTGGA CATCCTGCGG GGGGGCACGG AGCGCCTCTT CACAAACCTC 
AAGGACACCT CCTCCAAGGT CATCCAGTCT GTTGCCAATT ACGCAAAAGG AGATTTGGAC 
ATCTCTTACA TCACATCTAG AATTGCTGTG ATGTCGTTCC CAGCAGAAGG CGTGGAGTCG 
GCCATCAAGA ACAACATCGA AGACGTGCGG TTGTTTCTGG ACTCCAAGCA CCCGGGACAT 
TACGCCGTCT ACAACCTGTC CCCAAGAACA TACCGGCCCT CCAAATTTCA CAACCGGGTC 
TCCGAGTGTG GCTGGGCGGC CAGACGGGCC CCGAACCTTC GCAGCCTGTA CAGTGTGTGT 
CGGAACATGC ACTCGTGGCT CCGGCAGGAC CACAAGAACG TCTGTGTCGT GCACTGCATG 
GACGGGAGAG CTGCGTCTGC GGTGGCCGTG TGCTCCTTCC TGTGCTTCTG CCGCCTGTTC 
AGCACCGCGG AGGCTGCCGT GTACATGTTC AGCATGAAGC GCTGCCCGCC AGGCATTTGG 
CCATCCCACA AGAGGTACAT CGAGTACATG TGTGACATGG TGGCCGAGGA GCCCATCACA 
CCCCATAGCA AGCCCATCCT GGTGAAAGCC GTGGTCATGA CACCCGTGCC CCTGTTCAGC 
AAGCAGAGAA ACGGCTGCAG GCCCTTCTGC GAGGTCTACG TGGGGGATGA GCGCGTGACT 
ACCACGTCTC AGGAGTACGA CAAGATGAGG GATTTCAGAA TCGAGGATGG CAAAGCAGTC 
ATTCCCCTGG GCATAACAGT GCAAGGGGAC GTGCTCATTG TCATCTACCA CGCCAGGTCC 
ACCCTGGGAG GAAGACTGCA GGCCAAGGTG GCATCCATGA AGATGTTCCA GGTCCAGTTC 
CACACGGGGT TTGTGCCTCG GAATGCGACT ACTGTGAAGT TTGCAAAGTA TGACCTTGAT 
GCGTGTGACA TTCAGGAGAA ATACCCAGAT TTATTCCAAG TGAACGTGGA GGTGGAGGTG 
GAGCCCAGAG ACAGGCCCAG CCGGGAGGCC CCACCCTGGG AGAGCACCAG CATGAGGGGG 
CTCAACCCCA AAATCCTGTT TTCCAGCCGA GAGGAGCAGC AAGACATTCT GTCTAAATTT 
GGGAAGCCGG AGCTCCCCAG GCAGCCCGGC TCCACCGCTC AGTACGACGC CGAGGCAGGG 
TCCCTGGACG CAGAGCCCAC AGAGTCTGAT TCACCACAGA GCAGCGGCAC TGACACCAGC 
CACTTCCTCC ACACGCTGGA TTGGCACGAG GAGAAAGATA CAGAGACGAG TCCAGAAAGT 
CACTTTGCCA AGGAGAACCT ATCAACCCTG GCCGAGGATG CAGGTGGGAG TGAGCCTTCG 
GACGAGGAAG CAGCCACACC ATCCACCGAG AGCAGGGATG CTGCGGATGA GGACACACCA 
GGGCCAGCGG CGAGGCTGCC AGAGCGAGAT TTGATTTTTG ATGGGGCCAC GCCGGCCACA 
CCGCAGGAGC CCACACGGCA GGAGGACAGT GTCGACCTCC TAGGGCTGCA TGCCGAGGCT 
GGGCTGGCCC CCCCTGTGCA GGCCAGTGGG GCAGCCCCCA GCACCGCCGA CCTGCTCAGC 
CGCCTCCTGG GGGGCCCTGA CGCTGCTCCG GAGGTGCCCC CGGGTGACCT GCTGGGTGGA 
GAGGCTCCCC TCCTCTTCGC AAGCCCAGCC CCCTCCTCGA GCGCACAGAG CCCGCCCCGT 
GAGGGGCCAG CTGCCGACCC CTTTGACCCT CTCCTGCTGC CATCTGGGCC CAACACCCAG 
CCGTGCTCCC AGCCTGACCT GTTCGGTGAA TTTCTTCATT CGGAATCTCC TGCCGCCGTG 
CCTGCGTCCT TCCCGTCCAC CCACAGCGCC CCACCGCCCG CCTGCAGCAC CGACTTCCTG 
CACCTGGGAG ATCTACCAGC AGAGCCTGGC AAGATGACGG CCTCCACCAG CCACCCGGAT 
CTGCTGGGGG GGTGGGAGGC CTGGGCCGAG GCGCCGGCCC CCGCAGCTGC TGCAGAAGGT 
CCCTTCTTCT CTGCAGGAGG CCAGCCTGCC GCCCCCAGTG CCCCCGGGAG CTGGACCAAG 
TCTCAGAGCC TGGACCCGTT TGCTGACCTC GGTGACCTCA GTTCTGGCCT CCAAGGCTCA 
CCGGCTGGAT TTGCTCCAGG GGCTTTTGGC GCCAAGACGG CCCCCCCTCC CACGGGCGGC 
AGCTCCTGGC AGCAGACGGG TCGGCCCCCG GCCCAGGGCA CCCCGTGGCC CCCCCAGGCC 
AAGCCGCCCC CCAAGGCCTG TCCGCAGCCA AGGCCTAACT ATGCTGCCAA CGTGAGCGTG 
ATTGGCGGCC GAGAGGAGAG AGGGGTCCGG GCCCCCAGCT TCGGCCAGAA GCCGAGAGTG 
TCCGAGAGCG ACTTTGAGGA CCTGCTGTCC CACCAGGGCT TCTCGTCCAA GGCTGACCGG 
AAGGGGCCAA GGACCATGGC TGAGATGCGG CGGCAGGAGC AGGCGAGGGA CGCAGACCCG 
CTGAAGCTGA AGCTCCTGGA GTGGACAGAG GGCAAGGAGA GGAACATCCG CGCGCTGCTG 
TCGACCCTGC ACACCGTGCT GTGGGACGGC GAGAGCCGCT GGGCCCCCGT GGGCATGGCC 
GACCTGGTGA CCCCCGGGCA GGTGAAGAAG CAGTACCGCC GCGCCGTGCT GGTGGTGCAC 
CCGGACAAGG CCGCAGGGCA GCCCTACGAG CAGTATGCCA AGATGATCTT CATGGAGCTG 
AACGACGCGT GGGCCGAGTT TGAGAGCCAG GGCTCCCGGC CGCTCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAK ( XM_022417288.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022272996.1 Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OCe04653
Clone ID Related Accession (Same CDS sequence)	XM_005618687.2 , XM_005618689.2 , XM_005618687.3 , XM_022417295.1 , XM_005618689.3 , XM_022417288.1
Accession Version	XM_022417288.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	cyclin-G-associated kinase isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAAC TTTCTGGCCA CCCCAATATT GTCCAGTTTT GTTCTGCAGC GTCCATAGGG 
AAAGAGGAGT CGGACACTGG GCAGGCTGAG TTCCTGCTGC TCACCGAGCT CTGCAAAGGG 
CAGCTGGTGG ACTTTTTAAA GAAAATTGAA TCTAGAGGTC CGCTCTCGTG CGATACTGTT 
CTGAAGATTT TCTATCAGAC GTGCAGAGCG GTGCAGCACA TGCACAGACA AAAGCCGCCC 
ATCATCCATA GAGACCTCAA GGTGGAAAAC TTATTGCTCA GTAACCAAGG GACCATCAAG 
CTGTGTGACT TTGGCAGTGC CACGACCATA TCACATTACC CCGACTACAG CTGGAGCGCC 
CAGAGGCGGG CCCTGGTGGA GGAAGAGATC ACCAGGAACA CCACACCCAT GTACAGGACA 
CCGGAAATCG TGGACTTGTA CTCCAACTTC CCCATCGGCG AGAAGCAGGA CATCTGGGCG 
CTGGGCTGCA TCTTGTATCT GCTGTGCTTC CGGCAGCATC CTTTTGAAGA CGGAGCAAAG 
CTTCGGATAG TCAACGGGAA GTTCTCGATC CCCCCCAATG ACACCCGGTA CTCTGTGTTC 
CACGACCTCA TCCGCGCCAC GCTGAAGGTC AACCCCGAGG AGCGGCTGTC CATCACAGAA 
CTGGTGAACC AGCTCCAGGA GATCGCGGCC GCCAGGAACG TGAACCCCAA GTCTCCCATC 
ACTGAGCTCC TAGAGCAGAA CGGAGGCTAT GGGAACGCTG TCCCTTCCCG AGGGCCACCC 
CCTCCTGGGG GCTCCAGCAG CGGCTACAGC GGAGGCCTGG CGCTGGCCGA GTACGACCAG 
CCCTACGGCG GGCTCCTGGA CATCCTGCGG GGGGGCACGG AGCGCCTCTT CACAAACCTC 
AAGGACACCT CCTCCAAGGT CATCCAGTCT GTTGCCAATT ACGCAAAAGG AGATTTGGAC 
ATCTCTTACA TCACATCTAG AATTGCTGTG ATGTCGTTCC CAGCAGAAGG CGTGGAGTCG 
GCCATCAAGA ACAACATCGA AGACGTGCGG TTGTTTCTGG ACTCCAAGCA CCCGGGACAT 
TACGCCGTCT ACAACCTGTC CCCAAGAACA TACCGGCCCT CCAAATTTCA CAACCGGGTC 
TCCGAGTGTG GCTGGGCGGC CAGACGGGCC CCGAACCTTC GCAGCCTGTA CAGTGTGTGT 
CGGAACATGC ACTCGTGGCT CCGGCAGGAC CACAAGAACG TCTGTGTCGT GCACTGCATG 
GACGGGAGAG CTGCGTCTGC GGTGGCCGTG TGCTCCTTCC TGTGCTTCTG CCGCCTGTTC 
AGCACCGCGG AGGCTGCCGT GTACATGTTC AGCATGAAGC GCTGCCCGCC AGGCATTTGG 
CCATCCCACA AGAGGTACAT CGAGTACATG TGTGACATGG TGGCCGAGGA GCCCATCACA 
CCCCATAGCA AGCCCATCCT GGTGAAAGCC GTGGTCATGA CACCCGTGCC CCTGTTCAGC 
AAGCAGAGAA ACGGCTGCAG GCCCTTCTGC GAGGTCTACG TGGGGGATGA GCGCGTGACT 
ACCACGTCTC AGGAGTACGA CAAGATGAGG GATTTCAGAA TCGAGGATGG CAAAGCAGTC 
ATTCCCCTGG GCATAACAGT GCAAGGGGAC GTGCTCATTG TCATCTACCA CGCCAGGTCC 
ACCCTGGGAG GAAGACTGCA GGCCAAGGTG GCATCCATGA AGATGTTCCA GGTCCAGTTC 
CACACGGGGT TTGTGCCTCG GAATGCGACT ACTGTGAAGT TTGCAAAGTA TGACCTTGAT 
GCGTGTGACA TTCAGGAGAA ATACCCAGAT TTATTCCAAG TGAACGTGGA GGTGGAGGTG 
GAGCCCAGAG ACAGGCCCAG CCGGGAGGCC CCACCCTGGG AGAGCACCAG CATGAGGGGG 
CTCAACCCCA AAATCCTGTT TTCCAGCCGA GAGGAGCAGC AAGACATTCT GTCTAAATTT 
GGGAAGCCGG AGCTCCCCAG GCAGCCCGGC TCCACCGCTC AGTACGACGC CGAGGCAGGG 
TCCCTGGACG CAGAGCCCAC AGAGTCTGAT TCACCACAGA GCAGCGGCAC TGACACCAGC 
CACTTCCTCC ACACGCTGGA TTGGCACGAG GAGAAAGATA CAGAGACGAG TCCAGAAAGT 
CACTTTGCCA AGGAGAACCT ATCAACCCTG GCCGAGGATG CAGGTGGGAG TGAGCCTTCG 
GACGAGGAAG CAGCCACACC ATCCACCGAG AGCAGGGATG CTGCGGATGA GGACACACCA 
GGGCCAGCGG CGAGGCTGCC AGAGCGAGAT TTGATTTTTG ATGGGGCCAC GCCGGCCACA 
CCGCAGGAGC CCACACGGCA GGAGGACAGT GTCGACCTCC TAGGGCTGCA TGCCGAGGCT 
GGGCTGGCCC CCCCTGTGCA GGCCAGTGGG GCAGCCCCCA GCACCGCCGA CCTGCTCAGC 
CGCCTCCTGG GGGGCCCTGA CGCTGCTCCG GAGGTGCCCC CGGGTGACCT GCTGGGTGGA 
GAGGCTCCCC TCCTCTTCGC AAGCCCAGCC CCCTCCTCGA GCGCACAGAG CCCGCCCCGT 
GAGGGGCCAG CTGCCGACCC CTTTGACCCT CTCCTGCTGC CATCTGGGCC CAACACCCAG 
CCGTGCTCCC AGCCTGACCT GTTCGGTGAA TTTCTTCATT CGGAATCTCC TGCCGCCGTG 
CCTGCGTCCT TCCCGTCCAC CCACAGCGCC CCACCGCCCG CCTGCAGCAC CGACTTCCTG 
CACCTGGGAG ATCTACCAGC AGAGCCTGGC AAGATGACGG CCTCCACCAG CCACCCGGAT 
CTGCTGGGGG GGTGGGAGGC CTGGGCCGAG GCGCCGGCCC CCGCAGCTGC TGCAGAAGGT 
CCCTTCTTCT CTGCAGGAGG CCAGCCTGCC GCCCCCAGTG CCCCCGGGAG CTGGACCAAG 
TCTCAGAGCC TGGACCCGTT TGCTGACCTC GGTGACCTCA GTTCTGGCCT CCAAGGCTCA 
CCGGCTGGAT TTGCTCCAGG GGCTTTTGGC GCCAAGACGG CCCCCCCTCC CACGGGCGGC 
AGCTCCTGGC AGCAGACGGG TCGGCCCCCG GCCCAGGGCA CCCCGTGGCC CCCCCAGGCC 
AAGCCGCCCC CCAAGGCCTG TCCGCAGCCA AGGCCTAACT ATGCTGCCAA CGTGAGCGTG 
ATTGGCGGCC GAGAGGAGAG AGGGGTCCGG GCCCCCAGCT TCGGCCAGAA GCCGAGAGTG 
TCCGAGAGCG ACTTTGAGGA CCTGCTGTCC CACCAGGGCT TCTCGTCCAA GGCTGACCGG 
AAGGGGCCAA GGACCATGGC TGAGATGCGG CGGCAGGAGC AGGCGAGGGA CGCAGACCCG 
CTGAAGCTGA AGCTCCTGGA GTGGACAGAG GGCAAGGAGA GGAACATCCG CGCGCTGCTG 
TCGACCCTGC ACACCGTGCT GTGGGACGGC GAGAGCCGCT GGGCCCCCGT GGGCATGGCC 
GACCTGGTGA CCCCCGGGCA GGTGAAGAAG CAGTACCGCC GCGCCGTGCT GGTGGTGCAC 
CCGGACAAGG CCGCAGGGCA GCCCTACGAG CAGTATGCCA AGATGATCTT CATGGAGCTG 
AACGACGCGT GGGCCGAGTT TGAGAGCCAG GGCTCCCGGC CGCTCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022272996.1
CDS	240..3890
Translation	MKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLTELCKGQLVDFLKKIESRGPLSCDTVLKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKFSIPPNDTRYSVFHDLIRATLKVNPEERLSITELVNQLQEIAAARNVNPKSPITELLEQNGGYGNAVPSRGPPPPGGSSSGYSGGLALAEYDQPYGGLLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRLFLDSKHPGHYAVYNLSPRTYRPSKFHNRVSECGWAARRAPNLRSLYSVCRNMHSWLRQDHKNVCVVHCMDGRAASAVAVCSFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYMCDMVAEEPITPHSKPILVKAVVMTPVPLFSKQRNGCRPFCEVYVGDERVTTTSQEYDKMRDFRIEDGKAVIPLGITVQGDVLIVIYHARSTLGGRLQAKVASMKMFQVQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNVEVEVEPRDRPSREAPPWESTSMRGLNPKILFSSREEQQDILSKFGKPELPRQPGSTAQYDAEAGSLDAEPTESDSPQSSGTDTSHFLHTLDWHEEKDTETSPESHFAKENLSTLAEDAGGSEPSDEEAATPSTESRDAADEDTPGPAARLPERDLIFDGATPATPQEPTRQEDSVDLLGLHAEAGLAPPVQASGAAPSTADLLSRLLGGPDAAPEVPPGDLLGGEAPLLFASPAPSSSAQSPPREGPAADPFDPLLLPSGPNTQPCSQPDLFGEFLHSESPAAVPASFPSTHSAPPPACSTDFLHLGDLPAEPGKMTASTSHPDLLGGWEAWAEAPAPAAAAEGPFFSAGGQPAAPSAPGSWTKSQSLDPFADLGDLSSGLQGSPAGFAPGAFGAKTAPPPTGGSSWQQTGRPPAQGTPWPPQAKPPPKACPQPRPNYAANVSVIGGREERGVRAPSFGQKPRVSESDFEDLLSHQGFSSKADRKGPRTMAEMRRQEQARDADPLKLKLLEWTEGKERNIRALLSTLHTVLWDGESRWAPVGMADLVTPGQVKKQYRRAVLVVHPDKAAGQPYEQYAKMIFMELNDAWAEFESQGSRPLF

Target ORF information:

RefSeq Version	XM_022417288.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022417288.1

ORF Insert Sequence:

ATGAAAAAAC TTTCTGGCCA CCCCAATATT GTCCAGTTTT GTTCTGCAGC GTCCATAGGG 
AAAGAGGAGT CGGACACTGG GCAGGCTGAG TTCCTGCTGC TCACCGAGCT CTGCAAAGGG 
CAGCTGGTGG ACTTTTTAAA GAAAATTGAA TCTAGAGGTC CGCTCTCGTG CGATACTGTT 
CTGAAGATTT TCTATCAGAC GTGCAGAGCG GTGCAGCACA TGCACAGACA AAAGCCGCCC 
ATCATCCATA GAGACCTCAA GGTGGAAAAC TTATTGCTCA GTAACCAAGG GACCATCAAG 
CTGTGTGACT TTGGCAGTGC CACGACCATA TCACATTACC CCGACTACAG CTGGAGCGCC 
CAGAGGCGGG CCCTGGTGGA GGAAGAGATC ACCAGGAACA CCACACCCAT GTACAGGACA 
CCGGAAATCG TGGACTTGTA CTCCAACTTC CCCATCGGCG AGAAGCAGGA CATCTGGGCG 
CTGGGCTGCA TCTTGTATCT GCTGTGCTTC CGGCAGCATC CTTTTGAAGA CGGAGCAAAG 
CTTCGGATAG TCAACGGGAA GTTCTCGATC CCCCCCAATG ACACCCGGTA CTCTGTGTTC 
CACGACCTCA TCCGCGCCAC GCTGAAGGTC AACCCCGAGG AGCGGCTGTC CATCACAGAA 
CTGGTGAACC AGCTCCAGGA GATCGCGGCC GCCAGGAACG TGAACCCCAA GTCTCCCATC 
ACTGAGCTCC TAGAGCAGAA CGGAGGCTAT GGGAACGCTG TCCCTTCCCG AGGGCCACCC 
CCTCCTGGGG GCTCCAGCAG CGGCTACAGC GGAGGCCTGG CGCTGGCCGA GTACGACCAG 
CCCTACGGCG GGCTCCTGGA CATCCTGCGG GGGGGCACGG AGCGCCTCTT CACAAACCTC 
AAGGACACCT CCTCCAAGGT CATCCAGTCT GTTGCCAATT ACGCAAAAGG AGATTTGGAC 
ATCTCTTACA TCACATCTAG AATTGCTGTG ATGTCGTTCC CAGCAGAAGG CGTGGAGTCG 
GCCATCAAGA ACAACATCGA AGACGTGCGG TTGTTTCTGG ACTCCAAGCA CCCGGGACAT 
TACGCCGTCT ACAACCTGTC CCCAAGAACA TACCGGCCCT CCAAATTTCA CAACCGGGTC 
TCCGAGTGTG GCTGGGCGGC CAGACGGGCC CCGAACCTTC GCAGCCTGTA CAGTGTGTGT 
CGGAACATGC ACTCGTGGCT CCGGCAGGAC CACAAGAACG TCTGTGTCGT GCACTGCATG 
GACGGGAGAG CTGCGTCTGC GGTGGCCGTG TGCTCCTTCC TGTGCTTCTG CCGCCTGTTC 
AGCACCGCGG AGGCTGCCGT GTACATGTTC AGCATGAAGC GCTGCCCGCC AGGCATTTGG 
CCATCCCACA AGAGGTACAT CGAGTACATG TGTGACATGG TGGCCGAGGA GCCCATCACA 
CCCCATAGCA AGCCCATCCT GGTGAAAGCC GTGGTCATGA CACCCGTGCC CCTGTTCAGC 
AAGCAGAGAA ACGGCTGCAG GCCCTTCTGC GAGGTCTACG TGGGGGATGA GCGCGTGACT 
ACCACGTCTC AGGAGTACGA CAAGATGAGG GATTTCAGAA TCGAGGATGG CAAAGCAGTC 
ATTCCCCTGG GCATAACAGT GCAAGGGGAC GTGCTCATTG TCATCTACCA CGCCAGGTCC 
ACCCTGGGAG GAAGACTGCA GGCCAAGGTG GCATCCATGA AGATGTTCCA GGTCCAGTTC 
CACACGGGGT TTGTGCCTCG GAATGCGACT ACTGTGAAGT TTGCAAAGTA TGACCTTGAT 
GCGTGTGACA TTCAGGAGAA ATACCCAGAT TTATTCCAAG TGAACGTGGA GGTGGAGGTG 
GAGCCCAGAG ACAGGCCCAG CCGGGAGGCC CCACCCTGGG AGAGCACCAG CATGAGGGGG 
CTCAACCCCA AAATCCTGTT TTCCAGCCGA GAGGAGCAGC AAGACATTCT GTCTAAATTT 
GGGAAGCCGG AGCTCCCCAG GCAGCCCGGC TCCACCGCTC AGTACGACGC CGAGGCAGGG 
TCCCTGGACG CAGAGCCCAC AGAGTCTGAT TCACCACAGA GCAGCGGCAC TGACACCAGC 
CACTTCCTCC ACACGCTGGA TTGGCACGAG GAGAAAGATA CAGAGACGAG TCCAGAAAGT 
CACTTTGCCA AGGAGAACCT ATCAACCCTG GCCGAGGATG CAGGTGGGAG TGAGCCTTCG 
GACGAGGAAG CAGCCACACC ATCCACCGAG AGCAGGGATG CTGCGGATGA GGACACACCA 
GGGCCAGCGG CGAGGCTGCC AGAGCGAGAT TTGATTTTTG ATGGGGCCAC GCCGGCCACA 
CCGCAGGAGC CCACACGGCA GGAGGACAGT GTCGACCTCC TAGGGCTGCA TGCCGAGGCT 
GGGCTGGCCC CCCCTGTGCA GGCCAGTGGG GCAGCCCCCA GCACCGCCGA CCTGCTCAGC 
CGCCTCCTGG GGGGCCCTGA CGCTGCTCCG GAGGTGCCCC CGGGTGACCT GCTGGGTGGA 
GAGGCTCCCC TCCTCTTCGC AAGCCCAGCC CCCTCCTCGA GCGCACAGAG CCCGCCCCGT 
GAGGGGCCAG CTGCCGACCC CTTTGACCCT CTCCTGCTGC CATCTGGGCC CAACACCCAG 
CCGTGCTCCC AGCCTGACCT GTTCGGTGAA TTTCTTCATT CGGAATCTCC TGCCGCCGTG 
CCTGCGTCCT TCCCGTCCAC CCACAGCGCC CCACCGCCCG CCTGCAGCAC CGACTTCCTG 
CACCTGGGAG ATCTACCAGC AGAGCCTGGC AAGATGACGG CCTCCACCAG CCACCCGGAT 
CTGCTGGGGG GGTGGGAGGC CTGGGCCGAG GCGCCGGCCC CCGCAGCTGC TGCAGAAGGT 
CCCTTCTTCT CTGCAGGAGG CCAGCCTGCC GCCCCCAGTG CCCCCGGGAG CTGGACCAAG 
TCTCAGAGCC TGGACCCGTT TGCTGACCTC GGTGACCTCA GTTCTGGCCT CCAAGGCTCA 
CCGGCTGGAT TTGCTCCAGG GGCTTTTGGC GCCAAGACGG CCCCCCCTCC CACGGGCGGC 
AGCTCCTGGC AGCAGACGGG TCGGCCCCCG GCCCAGGGCA CCCCGTGGCC CCCCCAGGCC 
AAGCCGCCCC CCAAGGCCTG TCCGCAGCCA AGGCCTAACT ATGCTGCCAA CGTGAGCGTG 
ATTGGCGGCC GAGAGGAGAG AGGGGTCCGG GCCCCCAGCT TCGGCCAGAA GCCGAGAGTG 
TCCGAGAGCG ACTTTGAGGA CCTGCTGTCC CACCAGGGCT TCTCGTCCAA GGCTGACCGG 
AAGGGGCCAA GGACCATGGC TGAGATGCGG CGGCAGGAGC AGGCGAGGGA CGCAGACCCG 
CTGAAGCTGA AGCTCCTGGA GTGGACAGAG GGCAAGGAGA GGAACATCCG CGCGCTGCTG 
TCGACCCTGC ACACCGTGCT GTGGGACGGC GAGAGCCGCT GGGCCCCCGT GGGCATGGCC 
GACCTGGTGA CCCCCGGGCA GGTGAAGAAG CAGTACCGCC GCGCCGTGCT GGTGGTGCAC 
CCGGACAAGG CCGCAGGGCA GCCCTACGAG CAGTATGCCA AGATGATCTT CATGGAGCTG 
AACGACGCGT GGGCCGAGTT TGAGAGCCAG GGCTCCCGGC CGCTCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAK ( XM_014112678.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013968153.1 Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X6, mRNA.

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CloneID	OCe04654
Clone ID Related Accession (Same CDS sequence)	XM_014112678.1 , XM_005618688.2 , XM_005618688.3 , XM_022417293.1
Accession Version	XM_014112678.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	cyclin-G-associated kinase isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACAGGA CACCGGAAAT CGTGGACTTG TACTCCAACT TCCCCATCGG CGAGAAGCAG 
GACATCTGGG CGCTGGGCTG CATCTTGTAT CTGCTGTGCT TCCGGCAGCA TCCTTTTGAA 
GACGGAGCAA AGCTTCGGAT AGTCAACGGG AAGTTCTCGA TCCCCCCCAA TGACACCCGG 
TACTCTGTGT TCCACGACCT CATCCGCGCC ACGCTGAAGG TCAACCCCGA GGAGCGGCTG 
TCCATCACAG AACTGGTGAA CCAGCTCCAG GAGATCGCGG CCGCCAGGAA CGTGAACCCC 
AAGTCTCCCA TCACTGAGCT CCTAGAGCAG AACGGAGGCT ATGGGAACGC TGTCCCTTCC 
CGAGGGCCAC CCCCTCCTGG GGGCTCCAGC AGCGGCTACA GCGGAGGCCT GGCGCTGGCC 
GAGTACGACC AGCCCTACGG CGGGCTCCTG GACATCCTGC GGGGGGGCAC GGAGCGCCTC 
TTCACAAACC TCAAGGACAC CTCCTCCAAG GTCATCCAGT CTGTTGCCAA TTACGCAAAA 
GGAGATTTGG ACATCTCTTA CATCACATCT AGAATTGCTG TGATGTCGTT CCCAGCAGAA 
GGCGTGGAGT CGGCCATCAA GAACAACATC GAAGACGTGC GGTTGTTTCT GGACTCCAAG 
CACCCGGGAC ATTACGCCGT CTACAACCTG TCCCCAAGAA CATACCGGCC CTCCAAATTT 
CACAACCGGG TCTCCGAGTG TGGCTGGGCG GCCAGACGGG CCCCGAACCT TCGCAGCCTG 
TACAGTGTGT GTCGGAACAT GCACTCGTGG CTCCGGCAGG ACCACAAGAA CGTCTGTGTC 
GTGCACTGCA TGGACGGGAG AGCTGCGTCT GCGGTGGCCG TGTGCTCCTT CCTGTGCTTC 
TGCCGCCTGT TCAGCACCGC GGAGGCTGCC GTGTACATGT TCAGCATGAA GCGCTGCCCG 
CCAGGCATTT GGCCATCCCA CAAGAGGTAC ATCGAGTACA TGTGTGACAT GGTGGCCGAG 
GAGCCCATCA CACCCCATAG CAAGCCCATC CTGGTGAAAG CCGTGGTCAT GACACCCGTG 
CCCCTGTTCA GCAAGCAGAG AAACGGCTGC AGGCCCTTCT GCGAGGTCTA CGTGGGGGAT 
GAGCGCGTGA CTACCACGTC TCAGGAGTAC GACAAGATGA GGGATTTCAG AATCGAGGAT 
GGCAAAGCAG TCATTCCCCT GGGCATAACA GTGCAAGGGG ACGTGCTCAT TGTCATCTAC 
CACGCCAGGT CCACCCTGGG AGGAAGACTG CAGGCCAAGG TGGCATCCAT GAAGATGTTC 
CAGGTCCAGT TCCACACGGG GTTTGTGCCT CGGAATGCGA CTACTGTGAA GTTTGCAAAG 
TATGACCTTG ATGCGTGTGA CATTCAGGAG AAATACCCAG ATTTATTCCA AGTGAACGTG 
GAGGTGGAGG TGGAGCCCAG AGACAGGCCC AGCCGGGAGG CCCCACCCTG GGAGAGCACC 
AGCATGAGGG GGCTCAACCC CAAAATCCTG TTTTCCAGCC GAGAGGAGCA GCAAGACATT 
CTGTCTAAAT TTGGGAAGCC GGAGCTCCCC AGGCAGCCCG GCTCCACCGC TCAGTACGAC 
GCCGAGGCAG GGTCCCTGGA CGCAGAGCCC ACAGAGTCTG ATTCACCACA GAGCAGCGGC 
ACTGACACCA GCCACTTCCT CCACACGCTG GATTGGCACG AGGAGAAAGA TACAGAGACG 
AGTCCAGAAA GTCACTTTGC CAAGGAGAAC CTATCAACCC TGGCCGAGGA TGCAGGTGGG 
AGTGAGCCTT CGGACGAGGA AGCAGCCACA CCATCCACCG AGAGCAGGGA TGCTGCGGAT 
GAGGACACAC CAGGGCCAGC GGCGAGGCTG CCAGAGCGAG ATTTGATTTT TGATGGGGCC 
ACGCCGGCCA CACCGCAGGA GCCCACACGG CAGGAGGACA GTGTCGACCT CCTAGGGCTG 
CATGCCGAGG CTGGGCTGGC CCCCCCTGTG CAGGCCAGTG GGGCAGCCCC CAGCACCGCC 
GACCTGCTCA GCCGCCTCCT GGGGGGCCCT GACGCTGCTC CGGAGGTGCC CCCGGGTGAC 
CTGCTGGGTG GAGAGGCTCC CCTCCTCTTC GCAAGCCCAG CCCCCTCCTC GAGCGCACAG 
AGCCCGCCCC GTGAGGGGCC AGCTGCCGAC CCCTTTGACC CTCTCCTGCT GCCATCTGGG 
CCCAACACCC AGCCGTGCTC CCAGCCTGAC CTGTTCGGTG AATTTCTTCA TTCGGAATCT 
CCTGCCGCCG TGCCTGCGTC CTTCCCGTCC ACCCACAGCG CCCCACCGCC CGCCTGCAGC 
ACCGACTTCC TGCACCTGGG AGATCTACCA GCAGAGCCTG GCAAGATGAC GGCCTCCACC 
AGCCACCCGG ATCTGCTGGG GGGGTGGGAG GCCTGGGCCG AGGCGCCGGC CCCCGCAGCT 
GCTGCAGAAG GTCCCTTCTT CTCTGCAGGA GGCCAGCCTG CCGCCCCCAG TGCCCCCGGG 
AGCTGGACCA AGTCTCAGAG CCTGGACCCG TTTGCTGACC TCGGTGACCT CAGTTCTGGC 
CTCCAAGGCT CACCGGCTGG ATTTGCTCCA GGGGCTTTTG GCGCCAAGAC GGCCCCCCCT 
CCCACGGGCG GCAGCTCCTG GCAGCAGACG GGTCGGCCCC CGGCCCAGGG CACCCCGTGG 
CCCCCCCAGG CCAAGCCGCC CCCCAAGGCC TGTCCGCAGC CAAGGCCTAA CTATGCTGCC 
AACGTGAGCG TGATTGGCGG CCGAGAGGAG AGAGGGGTCC GGGCCCCCAG CTTCGGCCAG 
AAGCCGAGAG TGTCCGAGAG CGACTTTGAG GACCTGCTGT CCCACCAGGG CTTCTCGTCC 
AAGGCTGACC GGAAGGGGCC AAGGACCATG GCTGAGATGC GGCGGCAGGA GCAGGCGAGG 
GACGCAGACC CGCTGAAGCT GAAGCTCCTG GAGTGGACAG AGGGCAAGGA GAGGAACATC 
CGCGCGCTGC TGTCGACCCT GCACACCGTG CTGTGGGACG GCGAGAGCCG CTGGGCCCCC 
GTGGGCATGG CCGACCTGGT GACCCCCGGG CAGGTGAAGA AGCAGTACCG CCGCGCCGTG 
CTGGTGGTGC ACCCGGACAA GGCCGCAGGG CAGCCCTACG AGCAGTATGC CAAGATGATC 
TTCATGGAGC TGAACGACGC GTGGGCCGAG TTTGAGAGCC AGGGCTCCCG GCCGCTCTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013968153.1
CDS	223..3465
Translation	MYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKFSIPPNDTRYSVFHDLIRATLKVNPEERLSITELVNQLQEIAAARNVNPKSPITELLEQNGGYGNAVPSRGPPPPGGSSSGYSGGLALAEYDQPYGGLLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRLFLDSKHPGHYAVYNLSPRTYRPSKFHNRVSECGWAARRAPNLRSLYSVCRNMHSWLRQDHKNVCVVHCMDGRAASAVAVCSFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYMCDMVAEEPITPHSKPILVKAVVMTPVPLFSKQRNGCRPFCEVYVGDERVTTTSQEYDKMRDFRIEDGKAVIPLGITVQGDVLIVIYHARSTLGGRLQAKVASMKMFQVQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNVEVEVEPRDRPSREAPPWESTSMRGLNPKILFSSREEQQDILSKFGKPELPRQPGSTAQYDAEAGSLDAEPTESDSPQSSGTDTSHFLHTLDWHEEKDTETSPESHFAKENLSTLAEDAGGSEPSDEEAATPSTESRDAADEDTPGPAARLPERDLIFDGATPATPQEPTRQEDSVDLLGLHAEAGLAPPVQASGAAPSTADLLSRLLGGPDAAPEVPPGDLLGGEAPLLFASPAPSSSAQSPPREGPAADPFDPLLLPSGPNTQPCSQPDLFGEFLHSESPAAVPASFPSTHSAPPPACSTDFLHLGDLPAEPGKMTASTSHPDLLGGWEAWAEAPAPAAAAEGPFFSAGGQPAAPSAPGSWTKSQSLDPFADLGDLSSGLQGSPAGFAPGAFGAKTAPPPTGGSSWQQTGRPPAQGTPWPPQAKPPPKACPQPRPNYAANVSVIGGREERGVRAPSFGQKPRVSESDFEDLLSHQGFSSKADRKGPRTMAEMRRQEQARDADPLKLKLLEWTEGKERNIRALLSTLHTVLWDGESRWAPVGMADLVTPGQVKKQYRRAVLVVHPDKAAGQPYEQYAKMIFMELNDAWAEFESQGSRPLF

Target ORF information:

RefSeq Version	XM_014112678.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014112678.1

ORF Insert Sequence:

ATGTACAGGA CACCGGAAAT CGTGGACTTG TACTCCAACT TCCCCATCGG CGAGAAGCAG 
GACATCTGGG CGCTGGGCTG CATCTTGTAT CTGCTGTGCT TCCGGCAGCA TCCTTTTGAA 
GACGGAGCAA AGCTTCGGAT AGTCAACGGG AAGTTCTCGA TCCCCCCCAA TGACACCCGG 
TACTCTGTGT TCCACGACCT CATCCGCGCC ACGCTGAAGG TCAACCCCGA GGAGCGGCTG 
TCCATCACAG AACTGGTGAA CCAGCTCCAG GAGATCGCGG CCGCCAGGAA CGTGAACCCC 
AAGTCTCCCA TCACTGAGCT CCTAGAGCAG AACGGAGGCT ATGGGAACGC TGTCCCTTCC 
CGAGGGCCAC CCCCTCCTGG GGGCTCCAGC AGCGGCTACA GCGGAGGCCT GGCGCTGGCC 
GAGTACGACC AGCCCTACGG CGGGCTCCTG GACATCCTGC GGGGGGGCAC GGAGCGCCTC 
TTCACAAACC TCAAGGACAC CTCCTCCAAG GTCATCCAGT CTGTTGCCAA TTACGCAAAA 
GGAGATTTGG ACATCTCTTA CATCACATCT AGAATTGCTG TGATGTCGTT CCCAGCAGAA 
GGCGTGGAGT CGGCCATCAA GAACAACATC GAAGACGTGC GGTTGTTTCT GGACTCCAAG 
CACCCGGGAC ATTACGCCGT CTACAACCTG TCCCCAAGAA CATACCGGCC CTCCAAATTT 
CACAACCGGG TCTCCGAGTG TGGCTGGGCG GCCAGACGGG CCCCGAACCT TCGCAGCCTG 
TACAGTGTGT GTCGGAACAT GCACTCGTGG CTCCGGCAGG ACCACAAGAA CGTCTGTGTC 
GTGCACTGCA TGGACGGGAG AGCTGCGTCT GCGGTGGCCG TGTGCTCCTT CCTGTGCTTC 
TGCCGCCTGT TCAGCACCGC GGAGGCTGCC GTGTACATGT TCAGCATGAA GCGCTGCCCG 
CCAGGCATTT GGCCATCCCA CAAGAGGTAC ATCGAGTACA TGTGTGACAT GGTGGCCGAG 
GAGCCCATCA CACCCCATAG CAAGCCCATC CTGGTGAAAG CCGTGGTCAT GACACCCGTG 
CCCCTGTTCA GCAAGCAGAG AAACGGCTGC AGGCCCTTCT GCGAGGTCTA CGTGGGGGAT 
GAGCGCGTGA CTACCACGTC TCAGGAGTAC GACAAGATGA GGGATTTCAG AATCGAGGAT 
GGCAAAGCAG TCATTCCCCT GGGCATAACA GTGCAAGGGG ACGTGCTCAT TGTCATCTAC 
CACGCCAGGT CCACCCTGGG AGGAAGACTG CAGGCCAAGG TGGCATCCAT GAAGATGTTC 
CAGGTCCAGT TCCACACGGG GTTTGTGCCT CGGAATGCGA CTACTGTGAA GTTTGCAAAG 
TATGACCTTG ATGCGTGTGA CATTCAGGAG AAATACCCAG ATTTATTCCA AGTGAACGTG 
GAGGTGGAGG TGGAGCCCAG AGACAGGCCC AGCCGGGAGG CCCCACCCTG GGAGAGCACC 
AGCATGAGGG GGCTCAACCC CAAAATCCTG TTTTCCAGCC GAGAGGAGCA GCAAGACATT 
CTGTCTAAAT TTGGGAAGCC GGAGCTCCCC AGGCAGCCCG GCTCCACCGC TCAGTACGAC 
GCCGAGGCAG GGTCCCTGGA CGCAGAGCCC ACAGAGTCTG ATTCACCACA GAGCAGCGGC 
ACTGACACCA GCCACTTCCT CCACACGCTG GATTGGCACG AGGAGAAAGA TACAGAGACG 
AGTCCAGAAA GTCACTTTGC CAAGGAGAAC CTATCAACCC TGGCCGAGGA TGCAGGTGGG 
AGTGAGCCTT CGGACGAGGA AGCAGCCACA CCATCCACCG AGAGCAGGGA TGCTGCGGAT 
GAGGACACAC CAGGGCCAGC GGCGAGGCTG CCAGAGCGAG ATTTGATTTT TGATGGGGCC 
ACGCCGGCCA CACCGCAGGA GCCCACACGG CAGGAGGACA GTGTCGACCT CCTAGGGCTG 
CATGCCGAGG CTGGGCTGGC CCCCCCTGTG CAGGCCAGTG GGGCAGCCCC CAGCACCGCC 
GACCTGCTCA GCCGCCTCCT GGGGGGCCCT GACGCTGCTC CGGAGGTGCC CCCGGGTGAC 
CTGCTGGGTG GAGAGGCTCC CCTCCTCTTC GCAAGCCCAG CCCCCTCCTC GAGCGCACAG 
AGCCCGCCCC GTGAGGGGCC AGCTGCCGAC CCCTTTGACC CTCTCCTGCT GCCATCTGGG 
CCCAACACCC AGCCGTGCTC CCAGCCTGAC CTGTTCGGTG AATTTCTTCA TTCGGAATCT 
CCTGCCGCCG TGCCTGCGTC CTTCCCGTCC ACCCACAGCG CCCCACCGCC CGCCTGCAGC 
ACCGACTTCC TGCACCTGGG AGATCTACCA GCAGAGCCTG GCAAGATGAC GGCCTCCACC 
AGCCACCCGG ATCTGCTGGG GGGGTGGGAG GCCTGGGCCG AGGCGCCGGC CCCCGCAGCT 
GCTGCAGAAG GTCCCTTCTT CTCTGCAGGA GGCCAGCCTG CCGCCCCCAG TGCCCCCGGG 
AGCTGGACCA AGTCTCAGAG CCTGGACCCG TTTGCTGACC TCGGTGACCT CAGTTCTGGC 
CTCCAAGGCT CACCGGCTGG ATTTGCTCCA GGGGCTTTTG GCGCCAAGAC GGCCCCCCCT 
CCCACGGGCG GCAGCTCCTG GCAGCAGACG GGTCGGCCCC CGGCCCAGGG CACCCCGTGG 
CCCCCCCAGG CCAAGCCGCC CCCCAAGGCC TGTCCGCAGC CAAGGCCTAA CTATGCTGCC 
AACGTGAGCG TGATTGGCGG CCGAGAGGAG AGAGGGGTCC GGGCCCCCAG CTTCGGCCAG 
AAGCCGAGAG TGTCCGAGAG CGACTTTGAG GACCTGCTGT CCCACCAGGG CTTCTCGTCC 
AAGGCTGACC GGAAGGGGCC AAGGACCATG GCTGAGATGC GGCGGCAGGA GCAGGCGAGG 
GACGCAGACC CGCTGAAGCT GAAGCTCCTG GAGTGGACAG AGGGCAAGGA GAGGAACATC 
CGCGCGCTGC TGTCGACCCT GCACACCGTG CTGTGGGACG GCGAGAGCCG CTGGGCCCCC 
GTGGGCATGG CCGACCTGGT GACCCCCGGG CAGGTGAAGA AGCAGTACCG CCGCGCCGTG 
CTGGTGGTGC ACCCGGACAA GGCCGCAGGG CAGCCCTACG AGCAGTATGC CAAGATGATC 
TTCATGGAGC TGAACGACGC GTGGGCCGAG TTTGAGAGCC AGGGCTCCCG GCCGCTCTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAK ( XM_005618688.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005618745.1 Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OCe04654
Clone ID Related Accession (Same CDS sequence)	XM_014112678.1 , XM_005618688.2 , XM_005618688.3 , XM_022417293.1
Accession Version	XM_005618688.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	cyclin-G-associated kinase isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005618688.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACAGGA CACCGGAAAT CGTGGACTTG TACTCCAACT TCCCCATCGG CGAGAAGCAG 
GACATCTGGG CGCTGGGCTG CATCTTGTAT CTGCTGTGCT TCCGGCAGCA TCCTTTTGAA 
GACGGAGCAA AGCTTCGGAT AGTCAACGGG AAGTTCTCGA TCCCCCCCAA TGACACCCGG 
TACTCTGTGT TCCACGACCT CATCCGCGCC ACGCTGAAGG TCAACCCCGA GGAGCGGCTG 
TCCATCACAG AACTGGTGAA CCAGCTCCAG GAGATCGCGG CCGCCAGGAA CGTGAACCCC 
AAGTCTCCCA TCACTGAGCT CCTAGAGCAG AACGGAGGCT ATGGGAACGC TGTCCCTTCC 
CGAGGGCCAC CCCCTCCTGG GGGCTCCAGC AGCGGCTACA GCGGAGGCCT GGCGCTGGCC 
GAGTACGACC AGCCCTACGG CGGGCTCCTG GACATCCTGC GGGGGGGCAC GGAGCGCCTC 
TTCACAAACC TCAAGGACAC CTCCTCCAAG GTCATCCAGT CTGTTGCCAA TTACGCAAAA 
GGAGATTTGG ACATCTCTTA CATCACATCT AGAATTGCTG TGATGTCGTT CCCAGCAGAA 
GGCGTGGAGT CGGCCATCAA GAACAACATC GAAGACGTGC GGTTGTTTCT GGACTCCAAG 
CACCCGGGAC ATTACGCCGT CTACAACCTG TCCCCAAGAA CATACCGGCC CTCCAAATTT 
CACAACCGGG TCTCCGAGTG TGGCTGGGCG GCCAGACGGG CCCCGAACCT TCGCAGCCTG 
TACAGTGTGT GTCGGAACAT GCACTCGTGG CTCCGGCAGG ACCACAAGAA CGTCTGTGTC 
GTGCACTGCA TGGACGGGAG AGCTGCGTCT GCGGTGGCCG TGTGCTCCTT CCTGTGCTTC 
TGCCGCCTGT TCAGCACCGC GGAGGCTGCC GTGTACATGT TCAGCATGAA GCGCTGCCCG 
CCAGGCATTT GGCCATCCCA CAAGAGGTAC ATCGAGTACA TGTGTGACAT GGTGGCCGAG 
GAGCCCATCA CACCCCATAG CAAGCCCATC CTGGTGAAAG CCGTGGTCAT GACACCCGTG 
CCCCTGTTCA GCAAGCAGAG AAACGGCTGC AGGCCCTTCT GCGAGGTCTA CGTGGGGGAT 
GAGCGCGTGA CTACCACGTC TCAGGAGTAC GACAAGATGA GGGATTTCAG AATCGAGGAT 
GGCAAAGCAG TCATTCCCCT GGGCATAACA GTGCAAGGGG ACGTGCTCAT TGTCATCTAC 
CACGCCAGGT CCACCCTGGG AGGAAGACTG CAGGCCAAGG TGGCATCCAT GAAGATGTTC 
CAGGTCCAGT TCCACACGGG GTTTGTGCCT CGGAATGCGA CTACTGTGAA GTTTGCAAAG 
TATGACCTTG ATGCGTGTGA CATTCAGGAG AAATACCCAG ATTTATTCCA AGTGAACGTG 
GAGGTGGAGG TGGAGCCCAG AGACAGGCCC AGCCGGGAGG CCCCACCCTG GGAGAGCACC 
AGCATGAGGG GGCTCAACCC CAAAATCCTG TTTTCCAGCC GAGAGGAGCA GCAAGACATT 
CTGTCTAAAT TTGGGAAGCC GGAGCTCCCC AGGCAGCCCG GCTCCACCGC TCAGTACGAC 
GCCGAGGCAG GGTCCCTGGA CGCAGAGCCC ACAGAGTCTG ATTCACCACA GAGCAGCGGC 
ACTGACACCA GCCACTTCCT CCACACGCTG GATTGGCACG AGGAGAAAGA TACAGAGACG 
AGTCCAGAAA GTCACTTTGC CAAGGAGAAC CTATCAACCC TGGCCGAGGA TGCAGGTGGG 
AGTGAGCCTT CGGACGAGGA AGCAGCCACA CCATCCACCG AGAGCAGGGA TGCTGCGGAT 
GAGGACACAC CAGGGCCAGC GGCGAGGCTG CCAGAGCGAG ATTTGATTTT TGATGGGGCC 
ACGCCGGCCA CACCGCAGGA GCCCACACGG CAGGAGGACA GTGTCGACCT CCTAGGGCTG 
CATGCCGAGG CTGGGCTGGC CCCCCCTGTG CAGGCCAGTG GGGCAGCCCC CAGCACCGCC 
GACCTGCTCA GCCGCCTCCT GGGGGGCCCT GACGCTGCTC CGGAGGTGCC CCCGGGTGAC 
CTGCTGGGTG GAGAGGCTCC CCTCCTCTTC GCAAGCCCAG CCCCCTCCTC GAGCGCACAG 
AGCCCGCCCC GTGAGGGGCC AGCTGCCGAC CCCTTTGACC CTCTCCTGCT GCCATCTGGG 
CCCAACACCC AGCCGTGCTC CCAGCCTGAC CTGTTCGGTG AATTTCTTCA TTCGGAATCT 
CCTGCCGCCG TGCCTGCGTC CTTCCCGTCC ACCCACAGCG CCCCACCGCC CGCCTGCAGC 
ACCGACTTCC TGCACCTGGG AGATCTACCA GCAGAGCCTG GCAAGATGAC GGCCTCCACC 
AGCCACCCGG ATCTGCTGGG GGGGTGGGAG GCCTGGGCCG AGGCGCCGGC CCCCGCAGCT 
GCTGCAGAAG GTCCCTTCTT CTCTGCAGGA GGCCAGCCTG CCGCCCCCAG TGCCCCCGGG 
AGCTGGACCA AGTCTCAGAG CCTGGACCCG TTTGCTGACC TCGGTGACCT CAGTTCTGGC 
CTCCAAGGCT CACCGGCTGG ATTTGCTCCA GGGGCTTTTG GCGCCAAGAC GGCCCCCCCT 
CCCACGGGCG GCAGCTCCTG GCAGCAGACG GGTCGGCCCC CGGCCCAGGG CACCCCGTGG 
CCCCCCCAGG CCAAGCCGCC CCCCAAGGCC TGTCCGCAGC CAAGGCCTAA CTATGCTGCC 
AACGTGAGCG TGATTGGCGG CCGAGAGGAG AGAGGGGTCC GGGCCCCCAG CTTCGGCCAG 
AAGCCGAGAG TGTCCGAGAG CGACTTTGAG GACCTGCTGT CCCACCAGGG CTTCTCGTCC 
AAGGCTGACC GGAAGGGGCC AAGGACCATG GCTGAGATGC GGCGGCAGGA GCAGGCGAGG 
GACGCAGACC CGCTGAAGCT GAAGCTCCTG GAGTGGACAG AGGGCAAGGA GAGGAACATC 
CGCGCGCTGC TGTCGACCCT GCACACCGTG CTGTGGGACG GCGAGAGCCG CTGGGCCCCC 
GTGGGCATGG CCGACCTGGT GACCCCCGGG CAGGTGAAGA AGCAGTACCG CCGCGCCGTG 
CTGGTGGTGC ACCCGGACAA GGCCGCAGGG CAGCCCTACG AGCAGTATGC CAAGATGATC 
TTCATGGAGC TGAACGACGC GTGGGCCGAG TTTGAGAGCC AGGGCTCCCG GCCGCTCTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005618745.1
CDS	236..3478
Translation	MYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKFSIPPNDTRYSVFHDLIRATLKVNPEERLSITELVNQLQEIAAARNVNPKSPITELLEQNGGYGNAVPSRGPPPPGGSSSGYSGGLALAEYDQPYGGLLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRLFLDSKHPGHYAVYNLSPRTYRPSKFHNRVSECGWAARRAPNLRSLYSVCRNMHSWLRQDHKNVCVVHCMDGRAASAVAVCSFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYMCDMVAEEPITPHSKPILVKAVVMTPVPLFSKQRNGCRPFCEVYVGDERVTTTSQEYDKMRDFRIEDGKAVIPLGITVQGDVLIVIYHARSTLGGRLQAKVASMKMFQVQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNVEVEVEPRDRPSREAPPWESTSMRGLNPKILFSSREEQQDILSKFGKPELPRQPGSTAQYDAEAGSLDAEPTESDSPQSSGTDTSHFLHTLDWHEEKDTETSPESHFAKENLSTLAEDAGGSEPSDEEAATPSTESRDAADEDTPGPAARLPERDLIFDGATPATPQEPTRQEDSVDLLGLHAEAGLAPPVQASGAAPSTADLLSRLLGGPDAAPEVPPGDLLGGEAPLLFASPAPSSSAQSPPREGPAADPFDPLLLPSGPNTQPCSQPDLFGEFLHSESPAAVPASFPSTHSAPPPACSTDFLHLGDLPAEPGKMTASTSHPDLLGGWEAWAEAPAPAAAAEGPFFSAGGQPAAPSAPGSWTKSQSLDPFADLGDLSSGLQGSPAGFAPGAFGAKTAPPPTGGSSWQQTGRPPAQGTPWPPQAKPPPKACPQPRPNYAANVSVIGGREERGVRAPSFGQKPRVSESDFEDLLSHQGFSSKADRKGPRTMAEMRRQEQARDADPLKLKLLEWTEGKERNIRALLSTLHTVLWDGESRWAPVGMADLVTPGQVKKQYRRAVLVVHPDKAAGQPYEQYAKMIFMELNDAWAEFESQGSRPLF

Target ORF information:

RefSeq Version	XM_005618688.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005618688.2

ORF Insert Sequence:

ATGTACAGGA CACCGGAAAT CGTGGACTTG TACTCCAACT TCCCCATCGG CGAGAAGCAG 
GACATCTGGG CGCTGGGCTG CATCTTGTAT CTGCTGTGCT TCCGGCAGCA TCCTTTTGAA 
GACGGAGCAA AGCTTCGGAT AGTCAACGGG AAGTTCTCGA TCCCCCCCAA TGACACCCGG 
TACTCTGTGT TCCACGACCT CATCCGCGCC ACGCTGAAGG TCAACCCCGA GGAGCGGCTG 
TCCATCACAG AACTGGTGAA CCAGCTCCAG GAGATCGCGG CCGCCAGGAA CGTGAACCCC 
AAGTCTCCCA TCACTGAGCT CCTAGAGCAG AACGGAGGCT ATGGGAACGC TGTCCCTTCC 
CGAGGGCCAC CCCCTCCTGG GGGCTCCAGC AGCGGCTACA GCGGAGGCCT GGCGCTGGCC 
GAGTACGACC AGCCCTACGG CGGGCTCCTG GACATCCTGC GGGGGGGCAC GGAGCGCCTC 
TTCACAAACC TCAAGGACAC CTCCTCCAAG GTCATCCAGT CTGTTGCCAA TTACGCAAAA 
GGAGATTTGG ACATCTCTTA CATCACATCT AGAATTGCTG TGATGTCGTT CCCAGCAGAA 
GGCGTGGAGT CGGCCATCAA GAACAACATC GAAGACGTGC GGTTGTTTCT GGACTCCAAG 
CACCCGGGAC ATTACGCCGT CTACAACCTG TCCCCAAGAA CATACCGGCC CTCCAAATTT 
CACAACCGGG TCTCCGAGTG TGGCTGGGCG GCCAGACGGG CCCCGAACCT TCGCAGCCTG 
TACAGTGTGT GTCGGAACAT GCACTCGTGG CTCCGGCAGG ACCACAAGAA CGTCTGTGTC 
GTGCACTGCA TGGACGGGAG AGCTGCGTCT GCGGTGGCCG TGTGCTCCTT CCTGTGCTTC 
TGCCGCCTGT TCAGCACCGC GGAGGCTGCC GTGTACATGT TCAGCATGAA GCGCTGCCCG 
CCAGGCATTT GGCCATCCCA CAAGAGGTAC ATCGAGTACA TGTGTGACAT GGTGGCCGAG 
GAGCCCATCA CACCCCATAG CAAGCCCATC CTGGTGAAAG CCGTGGTCAT GACACCCGTG 
CCCCTGTTCA GCAAGCAGAG AAACGGCTGC AGGCCCTTCT GCGAGGTCTA CGTGGGGGAT 
GAGCGCGTGA CTACCACGTC TCAGGAGTAC GACAAGATGA GGGATTTCAG AATCGAGGAT 
GGCAAAGCAG TCATTCCCCT GGGCATAACA GTGCAAGGGG ACGTGCTCAT TGTCATCTAC 
CACGCCAGGT CCACCCTGGG AGGAAGACTG CAGGCCAAGG TGGCATCCAT GAAGATGTTC 
CAGGTCCAGT TCCACACGGG GTTTGTGCCT CGGAATGCGA CTACTGTGAA GTTTGCAAAG 
TATGACCTTG ATGCGTGTGA CATTCAGGAG AAATACCCAG ATTTATTCCA AGTGAACGTG 
GAGGTGGAGG TGGAGCCCAG AGACAGGCCC AGCCGGGAGG CCCCACCCTG GGAGAGCACC 
AGCATGAGGG GGCTCAACCC CAAAATCCTG TTTTCCAGCC GAGAGGAGCA GCAAGACATT 
CTGTCTAAAT TTGGGAAGCC GGAGCTCCCC AGGCAGCCCG GCTCCACCGC TCAGTACGAC 
GCCGAGGCAG GGTCCCTGGA CGCAGAGCCC ACAGAGTCTG ATTCACCACA GAGCAGCGGC 
ACTGACACCA GCCACTTCCT CCACACGCTG GATTGGCACG AGGAGAAAGA TACAGAGACG 
AGTCCAGAAA GTCACTTTGC CAAGGAGAAC CTATCAACCC TGGCCGAGGA TGCAGGTGGG 
AGTGAGCCTT CGGACGAGGA AGCAGCCACA CCATCCACCG AGAGCAGGGA TGCTGCGGAT 
GAGGACACAC CAGGGCCAGC GGCGAGGCTG CCAGAGCGAG ATTTGATTTT TGATGGGGCC 
ACGCCGGCCA CACCGCAGGA GCCCACACGG CAGGAGGACA GTGTCGACCT CCTAGGGCTG 
CATGCCGAGG CTGGGCTGGC CCCCCCTGTG CAGGCCAGTG GGGCAGCCCC CAGCACCGCC 
GACCTGCTCA GCCGCCTCCT GGGGGGCCCT GACGCTGCTC CGGAGGTGCC CCCGGGTGAC 
CTGCTGGGTG GAGAGGCTCC CCTCCTCTTC GCAAGCCCAG CCCCCTCCTC GAGCGCACAG 
AGCCCGCCCC GTGAGGGGCC AGCTGCCGAC CCCTTTGACC CTCTCCTGCT GCCATCTGGG 
CCCAACACCC AGCCGTGCTC CCAGCCTGAC CTGTTCGGTG AATTTCTTCA TTCGGAATCT 
CCTGCCGCCG TGCCTGCGTC CTTCCCGTCC ACCCACAGCG CCCCACCGCC CGCCTGCAGC 
ACCGACTTCC TGCACCTGGG AGATCTACCA GCAGAGCCTG GCAAGATGAC GGCCTCCACC 
AGCCACCCGG ATCTGCTGGG GGGGTGGGAG GCCTGGGCCG AGGCGCCGGC CCCCGCAGCT 
GCTGCAGAAG GTCCCTTCTT CTCTGCAGGA GGCCAGCCTG CCGCCCCCAG TGCCCCCGGG 
AGCTGGACCA AGTCTCAGAG CCTGGACCCG TTTGCTGACC TCGGTGACCT CAGTTCTGGC 
CTCCAAGGCT CACCGGCTGG ATTTGCTCCA GGGGCTTTTG GCGCCAAGAC GGCCCCCCCT 
CCCACGGGCG GCAGCTCCTG GCAGCAGACG GGTCGGCCCC CGGCCCAGGG CACCCCGTGG 
CCCCCCCAGG CCAAGCCGCC CCCCAAGGCC TGTCCGCAGC CAAGGCCTAA CTATGCTGCC 
AACGTGAGCG TGATTGGCGG CCGAGAGGAG AGAGGGGTCC GGGCCCCCAG CTTCGGCCAG 
AAGCCGAGAG TGTCCGAGAG CGACTTTGAG GACCTGCTGT CCCACCAGGG CTTCTCGTCC 
AAGGCTGACC GGAAGGGGCC AAGGACCATG GCTGAGATGC GGCGGCAGGA GCAGGCGAGG 
GACGCAGACC CGCTGAAGCT GAAGCTCCTG GAGTGGACAG AGGGCAAGGA GAGGAACATC 
CGCGCGCTGC TGTCGACCCT GCACACCGTG CTGTGGGACG GCGAGAGCCG CTGGGCCCCC 
GTGGGCATGG CCGACCTGGT GACCCCCGGG CAGGTGAAGA AGCAGTACCG CCGCGCCGTG 
CTGGTGGTGC ACCCGGACAA GGCCGCAGGG CAGCCCTACG AGCAGTATGC CAAGATGATC 
TTCATGGAGC TGAACGACGC GTGGGCCGAG TTTGAGAGCC AGGGCTCCCG GCCGCTCTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAK ( XM_005618688.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005618745.1 Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OCe04654
Clone ID Related Accession (Same CDS sequence)	XM_014112678.1 , XM_005618688.2 , XM_005618688.3 , XM_022417293.1
Accession Version	XM_005618688.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	cyclin-G-associated kinase isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005618688.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACAGGA CACCGGAAAT CGTGGACTTG TACTCCAACT TCCCCATCGG CGAGAAGCAG 
GACATCTGGG CGCTGGGCTG CATCTTGTAT CTGCTGTGCT TCCGGCAGCA TCCTTTTGAA 
GACGGAGCAA AGCTTCGGAT AGTCAACGGG AAGTTCTCGA TCCCCCCCAA TGACACCCGG 
TACTCTGTGT TCCACGACCT CATCCGCGCC ACGCTGAAGG TCAACCCCGA GGAGCGGCTG 
TCCATCACAG AACTGGTGAA CCAGCTCCAG GAGATCGCGG CCGCCAGGAA CGTGAACCCC 
AAGTCTCCCA TCACTGAGCT CCTAGAGCAG AACGGAGGCT ATGGGAACGC TGTCCCTTCC 
CGAGGGCCAC CCCCTCCTGG GGGCTCCAGC AGCGGCTACA GCGGAGGCCT GGCGCTGGCC 
GAGTACGACC AGCCCTACGG CGGGCTCCTG GACATCCTGC GGGGGGGCAC GGAGCGCCTC 
TTCACAAACC TCAAGGACAC CTCCTCCAAG GTCATCCAGT CTGTTGCCAA TTACGCAAAA 
GGAGATTTGG ACATCTCTTA CATCACATCT AGAATTGCTG TGATGTCGTT CCCAGCAGAA 
GGCGTGGAGT CGGCCATCAA GAACAACATC GAAGACGTGC GGTTGTTTCT GGACTCCAAG 
CACCCGGGAC ATTACGCCGT CTACAACCTG TCCCCAAGAA CATACCGGCC CTCCAAATTT 
CACAACCGGG TCTCCGAGTG TGGCTGGGCG GCCAGACGGG CCCCGAACCT TCGCAGCCTG 
TACAGTGTGT GTCGGAACAT GCACTCGTGG CTCCGGCAGG ACCACAAGAA CGTCTGTGTC 
GTGCACTGCA TGGACGGGAG AGCTGCGTCT GCGGTGGCCG TGTGCTCCTT CCTGTGCTTC 
TGCCGCCTGT TCAGCACCGC GGAGGCTGCC GTGTACATGT TCAGCATGAA GCGCTGCCCG 
CCAGGCATTT GGCCATCCCA CAAGAGGTAC ATCGAGTACA TGTGTGACAT GGTGGCCGAG 
GAGCCCATCA CACCCCATAG CAAGCCCATC CTGGTGAAAG CCGTGGTCAT GACACCCGTG 
CCCCTGTTCA GCAAGCAGAG AAACGGCTGC AGGCCCTTCT GCGAGGTCTA CGTGGGGGAT 
GAGCGCGTGA CTACCACGTC TCAGGAGTAC GACAAGATGA GGGATTTCAG AATCGAGGAT 
GGCAAAGCAG TCATTCCCCT GGGCATAACA GTGCAAGGGG ACGTGCTCAT TGTCATCTAC 
CACGCCAGGT CCACCCTGGG AGGAAGACTG CAGGCCAAGG TGGCATCCAT GAAGATGTTC 
CAGGTCCAGT TCCACACGGG GTTTGTGCCT CGGAATGCGA CTACTGTGAA GTTTGCAAAG 
TATGACCTTG ATGCGTGTGA CATTCAGGAG AAATACCCAG ATTTATTCCA AGTGAACGTG 
GAGGTGGAGG TGGAGCCCAG AGACAGGCCC AGCCGGGAGG CCCCACCCTG GGAGAGCACC 
AGCATGAGGG GGCTCAACCC CAAAATCCTG TTTTCCAGCC GAGAGGAGCA GCAAGACATT 
CTGTCTAAAT TTGGGAAGCC GGAGCTCCCC AGGCAGCCCG GCTCCACCGC TCAGTACGAC 
GCCGAGGCAG GGTCCCTGGA CGCAGAGCCC ACAGAGTCTG ATTCACCACA GAGCAGCGGC 
ACTGACACCA GCCACTTCCT CCACACGCTG GATTGGCACG AGGAGAAAGA TACAGAGACG 
AGTCCAGAAA GTCACTTTGC CAAGGAGAAC CTATCAACCC TGGCCGAGGA TGCAGGTGGG 
AGTGAGCCTT CGGACGAGGA AGCAGCCACA CCATCCACCG AGAGCAGGGA TGCTGCGGAT 
GAGGACACAC CAGGGCCAGC GGCGAGGCTG CCAGAGCGAG ATTTGATTTT TGATGGGGCC 
ACGCCGGCCA CACCGCAGGA GCCCACACGG CAGGAGGACA GTGTCGACCT CCTAGGGCTG 
CATGCCGAGG CTGGGCTGGC CCCCCCTGTG CAGGCCAGTG GGGCAGCCCC CAGCACCGCC 
GACCTGCTCA GCCGCCTCCT GGGGGGCCCT GACGCTGCTC CGGAGGTGCC CCCGGGTGAC 
CTGCTGGGTG GAGAGGCTCC CCTCCTCTTC GCAAGCCCAG CCCCCTCCTC GAGCGCACAG 
AGCCCGCCCC GTGAGGGGCC AGCTGCCGAC CCCTTTGACC CTCTCCTGCT GCCATCTGGG 
CCCAACACCC AGCCGTGCTC CCAGCCTGAC CTGTTCGGTG AATTTCTTCA TTCGGAATCT 
CCTGCCGCCG TGCCTGCGTC CTTCCCGTCC ACCCACAGCG CCCCACCGCC CGCCTGCAGC 
ACCGACTTCC TGCACCTGGG AGATCTACCA GCAGAGCCTG GCAAGATGAC GGCCTCCACC 
AGCCACCCGG ATCTGCTGGG GGGGTGGGAG GCCTGGGCCG AGGCGCCGGC CCCCGCAGCT 
GCTGCAGAAG GTCCCTTCTT CTCTGCAGGA GGCCAGCCTG CCGCCCCCAG TGCCCCCGGG 
AGCTGGACCA AGTCTCAGAG CCTGGACCCG TTTGCTGACC TCGGTGACCT CAGTTCTGGC 
CTCCAAGGCT CACCGGCTGG ATTTGCTCCA GGGGCTTTTG GCGCCAAGAC GGCCCCCCCT 
CCCACGGGCG GCAGCTCCTG GCAGCAGACG GGTCGGCCCC CGGCCCAGGG CACCCCGTGG 
CCCCCCCAGG CCAAGCCGCC CCCCAAGGCC TGTCCGCAGC CAAGGCCTAA CTATGCTGCC 
AACGTGAGCG TGATTGGCGG CCGAGAGGAG AGAGGGGTCC GGGCCCCCAG CTTCGGCCAG 
AAGCCGAGAG TGTCCGAGAG CGACTTTGAG GACCTGCTGT CCCACCAGGG CTTCTCGTCC 
AAGGCTGACC GGAAGGGGCC AAGGACCATG GCTGAGATGC GGCGGCAGGA GCAGGCGAGG 
GACGCAGACC CGCTGAAGCT GAAGCTCCTG GAGTGGACAG AGGGCAAGGA GAGGAACATC 
CGCGCGCTGC TGTCGACCCT GCACACCGTG CTGTGGGACG GCGAGAGCCG CTGGGCCCCC 
GTGGGCATGG CCGACCTGGT GACCCCCGGG CAGGTGAAGA AGCAGTACCG CCGCGCCGTG 
CTGGTGGTGC ACCCGGACAA GGCCGCAGGG CAGCCCTACG AGCAGTATGC CAAGATGATC 
TTCATGGAGC TGAACGACGC GTGGGCCGAG TTTGAGAGCC AGGGCTCCCG GCCGCTCTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005618745.1
CDS	278..3520
Translation	MYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKFSIPPNDTRYSVFHDLIRATLKVNPEERLSITELVNQLQEIAAARNVNPKSPITELLEQNGGYGNAVPSRGPPPPGGSSSGYSGGLALAEYDQPYGGLLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRLFLDSKHPGHYAVYNLSPRTYRPSKFHNRVSECGWAARRAPNLRSLYSVCRNMHSWLRQDHKNVCVVHCMDGRAASAVAVCSFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYMCDMVAEEPITPHSKPILVKAVVMTPVPLFSKQRNGCRPFCEVYVGDERVTTTSQEYDKMRDFRIEDGKAVIPLGITVQGDVLIVIYHARSTLGGRLQAKVASMKMFQVQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNVEVEVEPRDRPSREAPPWESTSMRGLNPKILFSSREEQQDILSKFGKPELPRQPGSTAQYDAEAGSLDAEPTESDSPQSSGTDTSHFLHTLDWHEEKDTETSPESHFAKENLSTLAEDAGGSEPSDEEAATPSTESRDAADEDTPGPAARLPERDLIFDGATPATPQEPTRQEDSVDLLGLHAEAGLAPPVQASGAAPSTADLLSRLLGGPDAAPEVPPGDLLGGEAPLLFASPAPSSSAQSPPREGPAADPFDPLLLPSGPNTQPCSQPDLFGEFLHSESPAAVPASFPSTHSAPPPACSTDFLHLGDLPAEPGKMTASTSHPDLLGGWEAWAEAPAPAAAAEGPFFSAGGQPAAPSAPGSWTKSQSLDPFADLGDLSSGLQGSPAGFAPGAFGAKTAPPPTGGSSWQQTGRPPAQGTPWPPQAKPPPKACPQPRPNYAANVSVIGGREERGVRAPSFGQKPRVSESDFEDLLSHQGFSSKADRKGPRTMAEMRRQEQARDADPLKLKLLEWTEGKERNIRALLSTLHTVLWDGESRWAPVGMADLVTPGQVKKQYRRAVLVVHPDKAAGQPYEQYAKMIFMELNDAWAEFESQGSRPLF

Target ORF information:

RefSeq Version	XM_005618688.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005618688.3

ORF Insert Sequence:

ATGTACAGGA CACCGGAAAT CGTGGACTTG TACTCCAACT TCCCCATCGG CGAGAAGCAG 
GACATCTGGG CGCTGGGCTG CATCTTGTAT CTGCTGTGCT TCCGGCAGCA TCCTTTTGAA 
GACGGAGCAA AGCTTCGGAT AGTCAACGGG AAGTTCTCGA TCCCCCCCAA TGACACCCGG 
TACTCTGTGT TCCACGACCT CATCCGCGCC ACGCTGAAGG TCAACCCCGA GGAGCGGCTG 
TCCATCACAG AACTGGTGAA CCAGCTCCAG GAGATCGCGG CCGCCAGGAA CGTGAACCCC 
AAGTCTCCCA TCACTGAGCT CCTAGAGCAG AACGGAGGCT ATGGGAACGC TGTCCCTTCC 
CGAGGGCCAC CCCCTCCTGG GGGCTCCAGC AGCGGCTACA GCGGAGGCCT GGCGCTGGCC 
GAGTACGACC AGCCCTACGG CGGGCTCCTG GACATCCTGC GGGGGGGCAC GGAGCGCCTC 
TTCACAAACC TCAAGGACAC CTCCTCCAAG GTCATCCAGT CTGTTGCCAA TTACGCAAAA 
GGAGATTTGG ACATCTCTTA CATCACATCT AGAATTGCTG TGATGTCGTT CCCAGCAGAA 
GGCGTGGAGT CGGCCATCAA GAACAACATC GAAGACGTGC GGTTGTTTCT GGACTCCAAG 
CACCCGGGAC ATTACGCCGT CTACAACCTG TCCCCAAGAA CATACCGGCC CTCCAAATTT 
CACAACCGGG TCTCCGAGTG TGGCTGGGCG GCCAGACGGG CCCCGAACCT TCGCAGCCTG 
TACAGTGTGT GTCGGAACAT GCACTCGTGG CTCCGGCAGG ACCACAAGAA CGTCTGTGTC 
GTGCACTGCA TGGACGGGAG AGCTGCGTCT GCGGTGGCCG TGTGCTCCTT CCTGTGCTTC 
TGCCGCCTGT TCAGCACCGC GGAGGCTGCC GTGTACATGT TCAGCATGAA GCGCTGCCCG 
CCAGGCATTT GGCCATCCCA CAAGAGGTAC ATCGAGTACA TGTGTGACAT GGTGGCCGAG 
GAGCCCATCA CACCCCATAG CAAGCCCATC CTGGTGAAAG CCGTGGTCAT GACACCCGTG 
CCCCTGTTCA GCAAGCAGAG AAACGGCTGC AGGCCCTTCT GCGAGGTCTA CGTGGGGGAT 
GAGCGCGTGA CTACCACGTC TCAGGAGTAC GACAAGATGA GGGATTTCAG AATCGAGGAT 
GGCAAAGCAG TCATTCCCCT GGGCATAACA GTGCAAGGGG ACGTGCTCAT TGTCATCTAC 
CACGCCAGGT CCACCCTGGG AGGAAGACTG CAGGCCAAGG TGGCATCCAT GAAGATGTTC 
CAGGTCCAGT TCCACACGGG GTTTGTGCCT CGGAATGCGA CTACTGTGAA GTTTGCAAAG 
TATGACCTTG ATGCGTGTGA CATTCAGGAG AAATACCCAG ATTTATTCCA AGTGAACGTG 
GAGGTGGAGG TGGAGCCCAG AGACAGGCCC AGCCGGGAGG CCCCACCCTG GGAGAGCACC 
AGCATGAGGG GGCTCAACCC CAAAATCCTG TTTTCCAGCC GAGAGGAGCA GCAAGACATT 
CTGTCTAAAT TTGGGAAGCC GGAGCTCCCC AGGCAGCCCG GCTCCACCGC TCAGTACGAC 
GCCGAGGCAG GGTCCCTGGA CGCAGAGCCC ACAGAGTCTG ATTCACCACA GAGCAGCGGC 
ACTGACACCA GCCACTTCCT CCACACGCTG GATTGGCACG AGGAGAAAGA TACAGAGACG 
AGTCCAGAAA GTCACTTTGC CAAGGAGAAC CTATCAACCC TGGCCGAGGA TGCAGGTGGG 
AGTGAGCCTT CGGACGAGGA AGCAGCCACA CCATCCACCG AGAGCAGGGA TGCTGCGGAT 
GAGGACACAC CAGGGCCAGC GGCGAGGCTG CCAGAGCGAG ATTTGATTTT TGATGGGGCC 
ACGCCGGCCA CACCGCAGGA GCCCACACGG CAGGAGGACA GTGTCGACCT CCTAGGGCTG 
CATGCCGAGG CTGGGCTGGC CCCCCCTGTG CAGGCCAGTG GGGCAGCCCC CAGCACCGCC 
GACCTGCTCA GCCGCCTCCT GGGGGGCCCT GACGCTGCTC CGGAGGTGCC CCCGGGTGAC 
CTGCTGGGTG GAGAGGCTCC CCTCCTCTTC GCAAGCCCAG CCCCCTCCTC GAGCGCACAG 
AGCCCGCCCC GTGAGGGGCC AGCTGCCGAC CCCTTTGACC CTCTCCTGCT GCCATCTGGG 
CCCAACACCC AGCCGTGCTC CCAGCCTGAC CTGTTCGGTG AATTTCTTCA TTCGGAATCT 
CCTGCCGCCG TGCCTGCGTC CTTCCCGTCC ACCCACAGCG CCCCACCGCC CGCCTGCAGC 
ACCGACTTCC TGCACCTGGG AGATCTACCA GCAGAGCCTG GCAAGATGAC GGCCTCCACC 
AGCCACCCGG ATCTGCTGGG GGGGTGGGAG GCCTGGGCCG AGGCGCCGGC CCCCGCAGCT 
GCTGCAGAAG GTCCCTTCTT CTCTGCAGGA GGCCAGCCTG CCGCCCCCAG TGCCCCCGGG 
AGCTGGACCA AGTCTCAGAG CCTGGACCCG TTTGCTGACC TCGGTGACCT CAGTTCTGGC 
CTCCAAGGCT CACCGGCTGG ATTTGCTCCA GGGGCTTTTG GCGCCAAGAC GGCCCCCCCT 
CCCACGGGCG GCAGCTCCTG GCAGCAGACG GGTCGGCCCC CGGCCCAGGG CACCCCGTGG 
CCCCCCCAGG CCAAGCCGCC CCCCAAGGCC TGTCCGCAGC CAAGGCCTAA CTATGCTGCC 
AACGTGAGCG TGATTGGCGG CCGAGAGGAG AGAGGGGTCC GGGCCCCCAG CTTCGGCCAG 
AAGCCGAGAG TGTCCGAGAG CGACTTTGAG GACCTGCTGT CCCACCAGGG CTTCTCGTCC 
AAGGCTGACC GGAAGGGGCC AAGGACCATG GCTGAGATGC GGCGGCAGGA GCAGGCGAGG 
GACGCAGACC CGCTGAAGCT GAAGCTCCTG GAGTGGACAG AGGGCAAGGA GAGGAACATC 
CGCGCGCTGC TGTCGACCCT GCACACCGTG CTGTGGGACG GCGAGAGCCG CTGGGCCCCC 
GTGGGCATGG CCGACCTGGT GACCCCCGGG CAGGTGAAGA AGCAGTACCG CCGCGCCGTG 
CTGGTGGTGC ACCCGGACAA GGCCGCAGGG CAGCCCTACG AGCAGTATGC CAAGATGATC 
TTCATGGAGC TGAACGACGC GTGGGCCGAG TTTGAGAGCC AGGGCTCCCG GCCGCTCTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAK ( XM_022417293.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022273001.1 Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OCe04654
Clone ID Related Accession (Same CDS sequence)	XM_014112678.1 , XM_005618688.2 , XM_005618688.3 , XM_022417293.1
Accession Version	XM_022417293.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	cyclin-G-associated kinase isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACAGGA CACCGGAAAT CGTGGACTTG TACTCCAACT TCCCCATCGG CGAGAAGCAG 
GACATCTGGG CGCTGGGCTG CATCTTGTAT CTGCTGTGCT TCCGGCAGCA TCCTTTTGAA 
GACGGAGCAA AGCTTCGGAT AGTCAACGGG AAGTTCTCGA TCCCCCCCAA TGACACCCGG 
TACTCTGTGT TCCACGACCT CATCCGCGCC ACGCTGAAGG TCAACCCCGA GGAGCGGCTG 
TCCATCACAG AACTGGTGAA CCAGCTCCAG GAGATCGCGG CCGCCAGGAA CGTGAACCCC 
AAGTCTCCCA TCACTGAGCT CCTAGAGCAG AACGGAGGCT ATGGGAACGC TGTCCCTTCC 
CGAGGGCCAC CCCCTCCTGG GGGCTCCAGC AGCGGCTACA GCGGAGGCCT GGCGCTGGCC 
GAGTACGACC AGCCCTACGG CGGGCTCCTG GACATCCTGC GGGGGGGCAC GGAGCGCCTC 
TTCACAAACC TCAAGGACAC CTCCTCCAAG GTCATCCAGT CTGTTGCCAA TTACGCAAAA 
GGAGATTTGG ACATCTCTTA CATCACATCT AGAATTGCTG TGATGTCGTT CCCAGCAGAA 
GGCGTGGAGT CGGCCATCAA GAACAACATC GAAGACGTGC GGTTGTTTCT GGACTCCAAG 
CACCCGGGAC ATTACGCCGT CTACAACCTG TCCCCAAGAA CATACCGGCC CTCCAAATTT 
CACAACCGGG TCTCCGAGTG TGGCTGGGCG GCCAGACGGG CCCCGAACCT TCGCAGCCTG 
TACAGTGTGT GTCGGAACAT GCACTCGTGG CTCCGGCAGG ACCACAAGAA CGTCTGTGTC 
GTGCACTGCA TGGACGGGAG AGCTGCGTCT GCGGTGGCCG TGTGCTCCTT CCTGTGCTTC 
TGCCGCCTGT TCAGCACCGC GGAGGCTGCC GTGTACATGT TCAGCATGAA GCGCTGCCCG 
CCAGGCATTT GGCCATCCCA CAAGAGGTAC ATCGAGTACA TGTGTGACAT GGTGGCCGAG 
GAGCCCATCA CACCCCATAG CAAGCCCATC CTGGTGAAAG CCGTGGTCAT GACACCCGTG 
CCCCTGTTCA GCAAGCAGAG AAACGGCTGC AGGCCCTTCT GCGAGGTCTA CGTGGGGGAT 
GAGCGCGTGA CTACCACGTC TCAGGAGTAC GACAAGATGA GGGATTTCAG AATCGAGGAT 
GGCAAAGCAG TCATTCCCCT GGGCATAACA GTGCAAGGGG ACGTGCTCAT TGTCATCTAC 
CACGCCAGGT CCACCCTGGG AGGAAGACTG CAGGCCAAGG TGGCATCCAT GAAGATGTTC 
CAGGTCCAGT TCCACACGGG GTTTGTGCCT CGGAATGCGA CTACTGTGAA GTTTGCAAAG 
TATGACCTTG ATGCGTGTGA CATTCAGGAG AAATACCCAG ATTTATTCCA AGTGAACGTG 
GAGGTGGAGG TGGAGCCCAG AGACAGGCCC AGCCGGGAGG CCCCACCCTG GGAGAGCACC 
AGCATGAGGG GGCTCAACCC CAAAATCCTG TTTTCCAGCC GAGAGGAGCA GCAAGACATT 
CTGTCTAAAT TTGGGAAGCC GGAGCTCCCC AGGCAGCCCG GCTCCACCGC TCAGTACGAC 
GCCGAGGCAG GGTCCCTGGA CGCAGAGCCC ACAGAGTCTG ATTCACCACA GAGCAGCGGC 
ACTGACACCA GCCACTTCCT CCACACGCTG GATTGGCACG AGGAGAAAGA TACAGAGACG 
AGTCCAGAAA GTCACTTTGC CAAGGAGAAC CTATCAACCC TGGCCGAGGA TGCAGGTGGG 
AGTGAGCCTT CGGACGAGGA AGCAGCCACA CCATCCACCG AGAGCAGGGA TGCTGCGGAT 
GAGGACACAC CAGGGCCAGC GGCGAGGCTG CCAGAGCGAG ATTTGATTTT TGATGGGGCC 
ACGCCGGCCA CACCGCAGGA GCCCACACGG CAGGAGGACA GTGTCGACCT CCTAGGGCTG 
CATGCCGAGG CTGGGCTGGC CCCCCCTGTG CAGGCCAGTG GGGCAGCCCC CAGCACCGCC 
GACCTGCTCA GCCGCCTCCT GGGGGGCCCT GACGCTGCTC CGGAGGTGCC CCCGGGTGAC 
CTGCTGGGTG GAGAGGCTCC CCTCCTCTTC GCAAGCCCAG CCCCCTCCTC GAGCGCACAG 
AGCCCGCCCC GTGAGGGGCC AGCTGCCGAC CCCTTTGACC CTCTCCTGCT GCCATCTGGG 
CCCAACACCC AGCCGTGCTC CCAGCCTGAC CTGTTCGGTG AATTTCTTCA TTCGGAATCT 
CCTGCCGCCG TGCCTGCGTC CTTCCCGTCC ACCCACAGCG CCCCACCGCC CGCCTGCAGC 
ACCGACTTCC TGCACCTGGG AGATCTACCA GCAGAGCCTG GCAAGATGAC GGCCTCCACC 
AGCCACCCGG ATCTGCTGGG GGGGTGGGAG GCCTGGGCCG AGGCGCCGGC CCCCGCAGCT 
GCTGCAGAAG GTCCCTTCTT CTCTGCAGGA GGCCAGCCTG CCGCCCCCAG TGCCCCCGGG 
AGCTGGACCA AGTCTCAGAG CCTGGACCCG TTTGCTGACC TCGGTGACCT CAGTTCTGGC 
CTCCAAGGCT CACCGGCTGG ATTTGCTCCA GGGGCTTTTG GCGCCAAGAC GGCCCCCCCT 
CCCACGGGCG GCAGCTCCTG GCAGCAGACG GGTCGGCCCC CGGCCCAGGG CACCCCGTGG 
CCCCCCCAGG CCAAGCCGCC CCCCAAGGCC TGTCCGCAGC CAAGGCCTAA CTATGCTGCC 
AACGTGAGCG TGATTGGCGG CCGAGAGGAG AGAGGGGTCC GGGCCCCCAG CTTCGGCCAG 
AAGCCGAGAG TGTCCGAGAG CGACTTTGAG GACCTGCTGT CCCACCAGGG CTTCTCGTCC 
AAGGCTGACC GGAAGGGGCC AAGGACCATG GCTGAGATGC GGCGGCAGGA GCAGGCGAGG 
GACGCAGACC CGCTGAAGCT GAAGCTCCTG GAGTGGACAG AGGGCAAGGA GAGGAACATC 
CGCGCGCTGC TGTCGACCCT GCACACCGTG CTGTGGGACG GCGAGAGCCG CTGGGCCCCC 
GTGGGCATGG CCGACCTGGT GACCCCCGGG CAGGTGAAGA AGCAGTACCG CCGCGCCGTG 
CTGGTGGTGC ACCCGGACAA GGCCGCAGGG CAGCCCTACG AGCAGTATGC CAAGATGATC 
TTCATGGAGC TGAACGACGC GTGGGCCGAG TTTGAGAGCC AGGGCTCCCG GCCGCTCTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022273001.1
CDS	319..3561
Translation	MYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKFSIPPNDTRYSVFHDLIRATLKVNPEERLSITELVNQLQEIAAARNVNPKSPITELLEQNGGYGNAVPSRGPPPPGGSSSGYSGGLALAEYDQPYGGLLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRLFLDSKHPGHYAVYNLSPRTYRPSKFHNRVSECGWAARRAPNLRSLYSVCRNMHSWLRQDHKNVCVVHCMDGRAASAVAVCSFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYMCDMVAEEPITPHSKPILVKAVVMTPVPLFSKQRNGCRPFCEVYVGDERVTTTSQEYDKMRDFRIEDGKAVIPLGITVQGDVLIVIYHARSTLGGRLQAKVASMKMFQVQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNVEVEVEPRDRPSREAPPWESTSMRGLNPKILFSSREEQQDILSKFGKPELPRQPGSTAQYDAEAGSLDAEPTESDSPQSSGTDTSHFLHTLDWHEEKDTETSPESHFAKENLSTLAEDAGGSEPSDEEAATPSTESRDAADEDTPGPAARLPERDLIFDGATPATPQEPTRQEDSVDLLGLHAEAGLAPPVQASGAAPSTADLLSRLLGGPDAAPEVPPGDLLGGEAPLLFASPAPSSSAQSPPREGPAADPFDPLLLPSGPNTQPCSQPDLFGEFLHSESPAAVPASFPSTHSAPPPACSTDFLHLGDLPAEPGKMTASTSHPDLLGGWEAWAEAPAPAAAAEGPFFSAGGQPAAPSAPGSWTKSQSLDPFADLGDLSSGLQGSPAGFAPGAFGAKTAPPPTGGSSWQQTGRPPAQGTPWPPQAKPPPKACPQPRPNYAANVSVIGGREERGVRAPSFGQKPRVSESDFEDLLSHQGFSSKADRKGPRTMAEMRRQEQARDADPLKLKLLEWTEGKERNIRALLSTLHTVLWDGESRWAPVGMADLVTPGQVKKQYRRAVLVVHPDKAAGQPYEQYAKMIFMELNDAWAEFESQGSRPLF

Target ORF information:

RefSeq Version	XM_022417293.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022417293.1

ORF Insert Sequence:

ATGTACAGGA CACCGGAAAT CGTGGACTTG TACTCCAACT TCCCCATCGG CGAGAAGCAG 
GACATCTGGG CGCTGGGCTG CATCTTGTAT CTGCTGTGCT TCCGGCAGCA TCCTTTTGAA 
GACGGAGCAA AGCTTCGGAT AGTCAACGGG AAGTTCTCGA TCCCCCCCAA TGACACCCGG 
TACTCTGTGT TCCACGACCT CATCCGCGCC ACGCTGAAGG TCAACCCCGA GGAGCGGCTG 
TCCATCACAG AACTGGTGAA CCAGCTCCAG GAGATCGCGG CCGCCAGGAA CGTGAACCCC 
AAGTCTCCCA TCACTGAGCT CCTAGAGCAG AACGGAGGCT ATGGGAACGC TGTCCCTTCC 
CGAGGGCCAC CCCCTCCTGG GGGCTCCAGC AGCGGCTACA GCGGAGGCCT GGCGCTGGCC 
GAGTACGACC AGCCCTACGG CGGGCTCCTG GACATCCTGC GGGGGGGCAC GGAGCGCCTC 
TTCACAAACC TCAAGGACAC CTCCTCCAAG GTCATCCAGT CTGTTGCCAA TTACGCAAAA 
GGAGATTTGG ACATCTCTTA CATCACATCT AGAATTGCTG TGATGTCGTT CCCAGCAGAA 
GGCGTGGAGT CGGCCATCAA GAACAACATC GAAGACGTGC GGTTGTTTCT GGACTCCAAG 
CACCCGGGAC ATTACGCCGT CTACAACCTG TCCCCAAGAA CATACCGGCC CTCCAAATTT 
CACAACCGGG TCTCCGAGTG TGGCTGGGCG GCCAGACGGG CCCCGAACCT TCGCAGCCTG 
TACAGTGTGT GTCGGAACAT GCACTCGTGG CTCCGGCAGG ACCACAAGAA CGTCTGTGTC 
GTGCACTGCA TGGACGGGAG AGCTGCGTCT GCGGTGGCCG TGTGCTCCTT CCTGTGCTTC 
TGCCGCCTGT TCAGCACCGC GGAGGCTGCC GTGTACATGT TCAGCATGAA GCGCTGCCCG 
CCAGGCATTT GGCCATCCCA CAAGAGGTAC ATCGAGTACA TGTGTGACAT GGTGGCCGAG 
GAGCCCATCA CACCCCATAG CAAGCCCATC CTGGTGAAAG CCGTGGTCAT GACACCCGTG 
CCCCTGTTCA GCAAGCAGAG AAACGGCTGC AGGCCCTTCT GCGAGGTCTA CGTGGGGGAT 
GAGCGCGTGA CTACCACGTC TCAGGAGTAC GACAAGATGA GGGATTTCAG AATCGAGGAT 
GGCAAAGCAG TCATTCCCCT GGGCATAACA GTGCAAGGGG ACGTGCTCAT TGTCATCTAC 
CACGCCAGGT CCACCCTGGG AGGAAGACTG CAGGCCAAGG TGGCATCCAT GAAGATGTTC 
CAGGTCCAGT TCCACACGGG GTTTGTGCCT CGGAATGCGA CTACTGTGAA GTTTGCAAAG 
TATGACCTTG ATGCGTGTGA CATTCAGGAG AAATACCCAG ATTTATTCCA AGTGAACGTG 
GAGGTGGAGG TGGAGCCCAG AGACAGGCCC AGCCGGGAGG CCCCACCCTG GGAGAGCACC 
AGCATGAGGG GGCTCAACCC CAAAATCCTG TTTTCCAGCC GAGAGGAGCA GCAAGACATT 
CTGTCTAAAT TTGGGAAGCC GGAGCTCCCC AGGCAGCCCG GCTCCACCGC TCAGTACGAC 
GCCGAGGCAG GGTCCCTGGA CGCAGAGCCC ACAGAGTCTG ATTCACCACA GAGCAGCGGC 
ACTGACACCA GCCACTTCCT CCACACGCTG GATTGGCACG AGGAGAAAGA TACAGAGACG 
AGTCCAGAAA GTCACTTTGC CAAGGAGAAC CTATCAACCC TGGCCGAGGA TGCAGGTGGG 
AGTGAGCCTT CGGACGAGGA AGCAGCCACA CCATCCACCG AGAGCAGGGA TGCTGCGGAT 
GAGGACACAC CAGGGCCAGC GGCGAGGCTG CCAGAGCGAG ATTTGATTTT TGATGGGGCC 
ACGCCGGCCA CACCGCAGGA GCCCACACGG CAGGAGGACA GTGTCGACCT CCTAGGGCTG 
CATGCCGAGG CTGGGCTGGC CCCCCCTGTG CAGGCCAGTG GGGCAGCCCC CAGCACCGCC 
GACCTGCTCA GCCGCCTCCT GGGGGGCCCT GACGCTGCTC CGGAGGTGCC CCCGGGTGAC 
CTGCTGGGTG GAGAGGCTCC CCTCCTCTTC GCAAGCCCAG CCCCCTCCTC GAGCGCACAG 
AGCCCGCCCC GTGAGGGGCC AGCTGCCGAC CCCTTTGACC CTCTCCTGCT GCCATCTGGG 
CCCAACACCC AGCCGTGCTC CCAGCCTGAC CTGTTCGGTG AATTTCTTCA TTCGGAATCT 
CCTGCCGCCG TGCCTGCGTC CTTCCCGTCC ACCCACAGCG CCCCACCGCC CGCCTGCAGC 
ACCGACTTCC TGCACCTGGG AGATCTACCA GCAGAGCCTG GCAAGATGAC GGCCTCCACC 
AGCCACCCGG ATCTGCTGGG GGGGTGGGAG GCCTGGGCCG AGGCGCCGGC CCCCGCAGCT 
GCTGCAGAAG GTCCCTTCTT CTCTGCAGGA GGCCAGCCTG CCGCCCCCAG TGCCCCCGGG 
AGCTGGACCA AGTCTCAGAG CCTGGACCCG TTTGCTGACC TCGGTGACCT CAGTTCTGGC 
CTCCAAGGCT CACCGGCTGG ATTTGCTCCA GGGGCTTTTG GCGCCAAGAC GGCCCCCCCT 
CCCACGGGCG GCAGCTCCTG GCAGCAGACG GGTCGGCCCC CGGCCCAGGG CACCCCGTGG 
CCCCCCCAGG CCAAGCCGCC CCCCAAGGCC TGTCCGCAGC CAAGGCCTAA CTATGCTGCC 
AACGTGAGCG TGATTGGCGG CCGAGAGGAG AGAGGGGTCC GGGCCCCCAG CTTCGGCCAG 
AAGCCGAGAG TGTCCGAGAG CGACTTTGAG GACCTGCTGT CCCACCAGGG CTTCTCGTCC 
AAGGCTGACC GGAAGGGGCC AAGGACCATG GCTGAGATGC GGCGGCAGGA GCAGGCGAGG 
GACGCAGACC CGCTGAAGCT GAAGCTCCTG GAGTGGACAG AGGGCAAGGA GAGGAACATC 
CGCGCGCTGC TGTCGACCCT GCACACCGTG CTGTGGGACG GCGAGAGCCG CTGGGCCCCC 
GTGGGCATGG CCGACCTGGT GACCCCCGGG CAGGTGAAGA AGCAGTACCG CCGCGCCGTG 
CTGGTGGTGC ACCCGGACAA GGCCGCAGGG CAGCCCTACG AGCAGTATGC CAAGATGATC 
TTCATGGAGC TGAACGACGC GTGGGCCGAG TTTGAGAGCC AGGGCTCCCG GCCGCTCTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAK ( XM_022417285.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022272993.1 Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe287420
Clone ID Related Accession (Same CDS sequence)	XM_022417285.1
Accession Version	XM_022417285.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	cyclin-G-associated kinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAAG AAGCAAGTGG CAGTGCACCT GTTGGTGGAG CTTCCACAAG AGGGTTTGCA 
TTTGTGTATG AAGCTCAAGA TCTGGCAAGT GGCAGAGAAT ATGCCTTAAA GAGATTATTG 
TCTAACGAAG AGGAAAAGAA CAGAGCCATC ATTCAGGAAG TTTGTTTCAT GAAAAAACTT 
TCTGGCCACC CCAATATTGT CCAGTTTTGT TCTGCAGCGT CCATAGGGAA AGAGGAGTCG 
GACACTGGGC AGGCTGAGTT CCTGCTGCTC ACCGAGCTCT GCAAAGGGCA GCTGGTGGAC 
TTTTTAAAGA AAATTGAATC TAGAGGTCCG CTCTCGTGCG ATACTGTTCT GAAGATTTTC 
TATCAGACGT GCAGAGCGGT GCAGCACATG CACAGACAAA AGCCGCCCAT CATCCATAGA 
GACCTCAAGG TGGAAAACTT ATTGCTCAGT AACCAAGGGA CCATCAAGCT GTGTGACTTT 
GGCAGTGCCA CGACCATATC ACATTACCCC GACTACAGCT GGAGCGCCCA GAGGCGGGCC 
CTGGTGGAGG AAGAGATCAC CAGGAACACC ACACCCATGT ACAGGACACC GGAAATCGTG 
GACTTGTACT CCAACTTCCC CATCGGCGAG AAGCAGGACA TCTGGGCGCT GGGCTGCATC 
TTGTATCTGC TGTGCTTCCG GCAGCATCCT TTTGAAGACG GAGCAAAGCT TCGGATAGTC 
AACGGGAAGT TCTCGATCCC CCCCAATGAC ACCCGGTACT CTGTGTTCCA CGACCTCATC 
CGCGCCACGC TGAAGGTCAA CCCCGAGGAG CGGCTGTCCA TCACAGAACT GGTGAACCAG 
CTCCAGGAGA TCGCGGCCGC CAGGAACGTG AACCCCAAGT CTCCCATCAC TGAGCTCCTA 
GAGCAGAACG GAGGCTATGG GAACGCTGTC CCTTCCCGAG GGCCACCCCC TCCTGGGGGC 
TCCAGCAGCG GCTACAGCGG AGGCCTGGCG CTGGCCGAGT ACGACCAGCC CTACGGCGGG 
CTCCTGGACA TCCTGCGGGG GGGCACGGAG CGCCTCTTCA CAAACCTCAA GGACACCTCC 
TCCAAGGTCA TCCAGTCTGT TGCCAATTAC GCAAAAGGAG ATTTGGACAT CTCTTACATC 
ACATCTAGAA TTGCTGTGAT GTCGTTCCCA GCAGAAGGCG TGGAGTCGGC CATCAAGAAC 
AACATCGAAG ACGTGCGGTT GTTTCTGGAC TCCAAGCACC CGGGACATTA CGCCGTCTAC 
AACCTGTCCC CAAGAACATA CCGGCCCTCC AAATTTCACA ACCGGGTCTC CGAGTGTGGC 
TGGGCGGCCA GACGGGCCCC GAACCTTCGC AGCCTGTACA GTGTGTGTCG GAACATGCAC 
TCGTGGCTCC GGCAGGACCA CAAGAACGTC TGTGTCGTGC ACTGCATGGA CGGGAGAGCT 
GCGTCTGCGG TGGCCGTGTG CTCCTTCCTG TGCTTCTGCC GCCTGTTCAG CACCGCGGAG 
GCTGCCGTGT ACATGTTCAG CATGAAGCGC TGCCCGCCAG GCATTTGGCC ATCCCACAAG 
AGGTACATCG AGTACATGTG TGACATGGTG GCCGAGGAGC CCATCACACC CCATAGCAAG 
CCCATCCTGG TGAAAGCCGT GGTCATGACA CCCGTGCCCC TGTTCAGCAA GCAGAGAAAC 
GGCTGCAGGC CCTTCTGCGA GGTCTACGTG GGGGATGAGC GCGTGACTAC CACGTCTCAG 
GAGTACGACA AGATGAGGGA TTTCAGAATC GAGGATGGCA AAGCAGTCAT TCCCCTGGGC 
ATAACAGTGC AAGGGGACGT GCTCATTGTC ATCTACCACG CCAGGTCCAC CCTGGGAGGA 
AGACTGCAGG CCAAGGTGGC ATCCATGAAG ATGTTCCAGG TCCAGTTCCA CACGGGGTTT 
GTGCCTCGGA ATGCGACTAC TGTGAAGTTT GCAAAGTATG ACCTTGATGC GTGTGACATT 
CAGGAGAAAT ACCCAGATTT ATTCCAAGTG AACGTGGAGG TGGAGGTGGA GCCCAGAGAC 
AGGCCCAGCC GGGAGGCCCC ACCCTGGGAG AGCACCAGCA TGAGGGGGCT CAACCCCAAA 
ATCCTGTTTT CCAGCCGAGA GGAGCAGCAA GACATTCTGT CTAAATTTGG GAAGCCGGAG 
CTCCCCAGGC AGCCCGGCTC CACCGCTCAG TACGACGCCG AGGCAGGGTC CCTGGACGCA 
GAGCCCACAG AGTCTGATTC ACCACAGAGC AGCGGCACTG ACACCAGCCA CTTCCTCCAC 
ACGCTGGATT GGCACGAGGA GAAAGATACA GAGACGAGTC CAGAAAGTCA CTTTGCCAAG 
GAGAACCTAT CAACCCTGGC CGAGGATGCA GGTGGGAGTG AGCCTTCGGA CGAGGAAGCA 
GCCACACCAT CCACCGAGAG CAGGGATGCT GCGGATGAGG ACACACCAGG GCCAGCGGCG 
AGGCTGCCAG AGCGAGATTT GATTTTTGAT GGGGCCACGC CGGCCACACC GCAGGAGCCC 
ACACGGCAGG AGGACAGTGT CGACCTCCTA GGGCTGCATG CCGAGGCTGG GCTGGCCCCC 
CCTGTGCAGG CCAGTGGGGC AGCCCCCAGC ACCGCCGACC TGCTCAGCCG CCTCCTGGGG 
GGCCCTGACG CTGCTCCGGA GGTGCCCCCG GGTGACCTGC TGGGTGGAGA GGCTCCCCTC 
CTCTTCGCAA GCCCAGCCCC CTCCTCGAGC GCACAGAGCC CGCCCCGTGA GGGGCCAGCT 
GCCGACCCCT TTGACCCTCT CCTGCTGCCA TCTGGGCCCA ACACCCAGCC GTGCTCCCAG 
CCTGACCTGT TCGGTGAATT TCTTCATTCG GAATCTCCTG CCGCCGTGCC TGCGTCCTTC 
CCGTCCACCC ACAGCGCCCC ACCGCCCGCC TGCAGCACCG ACTTCCTGCA CCTGGGAGAT 
CTACCAGCAG AGCCTGGCAA GATGACGGCC TCCACCAGCC ACCCGGATCT GCTGGGGGGG 
TGGGAGGCCT GGGCCGAGGC GCCGGCCCCC GCAGCTGCTG CAGAAGGTCC CTTCTTCTCT 
GCAGGAGGCC AGCCTGCCGC CCCCAGTGCC CCCGGGAGCT GGACCAAGTC TCAGAGCCTG 
GACCCGTTTG CTGACCTCGG TGACCTCAGT TCTGGCCTCC AAGGCTCACC GGCTGGATTT 
GCTCCAGGGG CTTTTGGCGC CAAGACGGCC CCCCCTCCCA CGGGCGGCAG CTCCTGGCAG 
CAGACGGGTC GGCCCCCGGC CCAGGGCACC CCGTGGCCCC CCCAGGCCAA GCCGCCCCCC 
AAGGCCTGTC CGCAGCCAAG GCCTAACTAT GCTGCCAACG TGAGCGTGAT TGGCGGCCGA 
GAGGAGAGAG GGGTCCGGGC CCCCAGCTTC GGCCAGAAGC CGAGAGTGTC CGAGAGCGAC 
TTTGAGGACC TGCTGTCCCA CCAGGGCTTC TCGTCCAAGG CTGACCGGAA GGGGCCAAGG 
ACCATGGCTG AGATGCGGCG GCAGGAGCAG GCGAGGGACG CAGACCCGCT GAAGCTGAAG 
CTCCTGGAGT GGACAGAGGG CAAGGAGAGG AACATCCGCG CGCTGCTGTC GACCCTGCAC 
ACCGTGCTGT GGGACGGCGA GAGCCGCTGG GCCCCCGTGG GCATGGCCGA CCTGGTGACC 
CCCGGGCAGG TGAAGAAGCA GTACCGCCGC GCCGTGCTGG TGGTGCACCC GGACAAGGCC 
GCAGGGCAGC CCTACGAGCA GTATGCCAAG ATGATCTTCA TGGAGCTGAA CGACGCGTGG 
GCCGAGTTTG AGAGCCAGGG CTCCCGGCCG CTCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022272993.1
CDS	16..3834
Translation	MVKEASGSAPVGGASTRGFAFVYEAQDLASGREYALKRLLSNEEEKNRAIIQEVCFMKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLTELCKGQLVDFLKKIESRGPLSCDTVLKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKFSIPPNDTRYSVFHDLIRATLKVNPEERLSITELVNQLQEIAAARNVNPKSPITELLEQNGGYGNAVPSRGPPPPGGSSSGYSGGLALAEYDQPYGGLLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRLFLDSKHPGHYAVYNLSPRTYRPSKFHNRVSECGWAARRAPNLRSLYSVCRNMHSWLRQDHKNVCVVHCMDGRAASAVAVCSFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYMCDMVAEEPITPHSKPILVKAVVMTPVPLFSKQRNGCRPFCEVYVGDERVTTTSQEYDKMRDFRIEDGKAVIPLGITVQGDVLIVIYHARSTLGGRLQAKVASMKMFQVQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNVEVEVEPRDRPSREAPPWESTSMRGLNPKILFSSREEQQDILSKFGKPELPRQPGSTAQYDAEAGSLDAEPTESDSPQSSGTDTSHFLHTLDWHEEKDTETSPESHFAKENLSTLAEDAGGSEPSDEEAATPSTESRDAADEDTPGPAARLPERDLIFDGATPATPQEPTRQEDSVDLLGLHAEAGLAPPVQASGAAPSTADLLSRLLGGPDAAPEVPPGDLLGGEAPLLFASPAPSSSAQSPPREGPAADPFDPLLLPSGPNTQPCSQPDLFGEFLHSESPAAVPASFPSTHSAPPPACSTDFLHLGDLPAEPGKMTASTSHPDLLGGWEAWAEAPAPAAAAEGPFFSAGGQPAAPSAPGSWTKSQSLDPFADLGDLSSGLQGSPAGFAPGAFGAKTAPPPTGGSSWQQTGRPPAQGTPWPPQAKPPPKACPQPRPNYAANVSVIGGREERGVRAPSFGQKPRVSESDFEDLLSHQGFSSKADRKGPRTMAEMRRQEQARDADPLKLKLLEWTEGKERNIRALLSTLHTVLWDGESRWAPVGMADLVTPGQVKKQYRRAVLVVHPDKAAGQPYEQYAKMIFMELNDAWAEFESQGSRPLF

Target ORF information:

RefSeq Version	XM_022417285.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022417285.1

ORF Insert Sequence:

ATGGTGAAAG AAGCAAGTGG CAGTGCACCT GTTGGTGGAG CTTCCACAAG AGGGTTTGCA 
TTTGTGTATG AAGCTCAAGA TCTGGCAAGT GGCAGAGAAT ATGCCTTAAA GAGATTATTG 
TCTAACGAAG AGGAAAAGAA CAGAGCCATC ATTCAGGAAG TTTGTTTCAT GAAAAAACTT 
TCTGGCCACC CCAATATTGT CCAGTTTTGT TCTGCAGCGT CCATAGGGAA AGAGGAGTCG 
GACACTGGGC AGGCTGAGTT CCTGCTGCTC ACCGAGCTCT GCAAAGGGCA GCTGGTGGAC 
TTTTTAAAGA AAATTGAATC TAGAGGTCCG CTCTCGTGCG ATACTGTTCT GAAGATTTTC 
TATCAGACGT GCAGAGCGGT GCAGCACATG CACAGACAAA AGCCGCCCAT CATCCATAGA 
GACCTCAAGG TGGAAAACTT ATTGCTCAGT AACCAAGGGA CCATCAAGCT GTGTGACTTT 
GGCAGTGCCA CGACCATATC ACATTACCCC GACTACAGCT GGAGCGCCCA GAGGCGGGCC 
CTGGTGGAGG AAGAGATCAC CAGGAACACC ACACCCATGT ACAGGACACC GGAAATCGTG 
GACTTGTACT CCAACTTCCC CATCGGCGAG AAGCAGGACA TCTGGGCGCT GGGCTGCATC 
TTGTATCTGC TGTGCTTCCG GCAGCATCCT TTTGAAGACG GAGCAAAGCT TCGGATAGTC 
AACGGGAAGT TCTCGATCCC CCCCAATGAC ACCCGGTACT CTGTGTTCCA CGACCTCATC 
CGCGCCACGC TGAAGGTCAA CCCCGAGGAG CGGCTGTCCA TCACAGAACT GGTGAACCAG 
CTCCAGGAGA TCGCGGCCGC CAGGAACGTG AACCCCAAGT CTCCCATCAC TGAGCTCCTA 
GAGCAGAACG GAGGCTATGG GAACGCTGTC CCTTCCCGAG GGCCACCCCC TCCTGGGGGC 
TCCAGCAGCG GCTACAGCGG AGGCCTGGCG CTGGCCGAGT ACGACCAGCC CTACGGCGGG 
CTCCTGGACA TCCTGCGGGG GGGCACGGAG CGCCTCTTCA CAAACCTCAA GGACACCTCC 
TCCAAGGTCA TCCAGTCTGT TGCCAATTAC GCAAAAGGAG ATTTGGACAT CTCTTACATC 
ACATCTAGAA TTGCTGTGAT GTCGTTCCCA GCAGAAGGCG TGGAGTCGGC CATCAAGAAC 
AACATCGAAG ACGTGCGGTT GTTTCTGGAC TCCAAGCACC CGGGACATTA CGCCGTCTAC 
AACCTGTCCC CAAGAACATA CCGGCCCTCC AAATTTCACA ACCGGGTCTC CGAGTGTGGC 
TGGGCGGCCA GACGGGCCCC GAACCTTCGC AGCCTGTACA GTGTGTGTCG GAACATGCAC 
TCGTGGCTCC GGCAGGACCA CAAGAACGTC TGTGTCGTGC ACTGCATGGA CGGGAGAGCT 
GCGTCTGCGG TGGCCGTGTG CTCCTTCCTG TGCTTCTGCC GCCTGTTCAG CACCGCGGAG 
GCTGCCGTGT ACATGTTCAG CATGAAGCGC TGCCCGCCAG GCATTTGGCC ATCCCACAAG 
AGGTACATCG AGTACATGTG TGACATGGTG GCCGAGGAGC CCATCACACC CCATAGCAAG 
CCCATCCTGG TGAAAGCCGT GGTCATGACA CCCGTGCCCC TGTTCAGCAA GCAGAGAAAC 
GGCTGCAGGC CCTTCTGCGA GGTCTACGTG GGGGATGAGC GCGTGACTAC CACGTCTCAG 
GAGTACGACA AGATGAGGGA TTTCAGAATC GAGGATGGCA AAGCAGTCAT TCCCCTGGGC 
ATAACAGTGC AAGGGGACGT GCTCATTGTC ATCTACCACG CCAGGTCCAC CCTGGGAGGA 
AGACTGCAGG CCAAGGTGGC ATCCATGAAG ATGTTCCAGG TCCAGTTCCA CACGGGGTTT 
GTGCCTCGGA ATGCGACTAC TGTGAAGTTT GCAAAGTATG ACCTTGATGC GTGTGACATT 
CAGGAGAAAT ACCCAGATTT ATTCCAAGTG AACGTGGAGG TGGAGGTGGA GCCCAGAGAC 
AGGCCCAGCC GGGAGGCCCC ACCCTGGGAG AGCACCAGCA TGAGGGGGCT CAACCCCAAA 
ATCCTGTTTT CCAGCCGAGA GGAGCAGCAA GACATTCTGT CTAAATTTGG GAAGCCGGAG 
CTCCCCAGGC AGCCCGGCTC CACCGCTCAG TACGACGCCG AGGCAGGGTC CCTGGACGCA 
GAGCCCACAG AGTCTGATTC ACCACAGAGC AGCGGCACTG ACACCAGCCA CTTCCTCCAC 
ACGCTGGATT GGCACGAGGA GAAAGATACA GAGACGAGTC CAGAAAGTCA CTTTGCCAAG 
GAGAACCTAT CAACCCTGGC CGAGGATGCA GGTGGGAGTG AGCCTTCGGA CGAGGAAGCA 
GCCACACCAT CCACCGAGAG CAGGGATGCT GCGGATGAGG ACACACCAGG GCCAGCGGCG 
AGGCTGCCAG AGCGAGATTT GATTTTTGAT GGGGCCACGC CGGCCACACC GCAGGAGCCC 
ACACGGCAGG AGGACAGTGT CGACCTCCTA GGGCTGCATG CCGAGGCTGG GCTGGCCCCC 
CCTGTGCAGG CCAGTGGGGC AGCCCCCAGC ACCGCCGACC TGCTCAGCCG CCTCCTGGGG 
GGCCCTGACG CTGCTCCGGA GGTGCCCCCG GGTGACCTGC TGGGTGGAGA GGCTCCCCTC 
CTCTTCGCAA GCCCAGCCCC CTCCTCGAGC GCACAGAGCC CGCCCCGTGA GGGGCCAGCT 
GCCGACCCCT TTGACCCTCT CCTGCTGCCA TCTGGGCCCA ACACCCAGCC GTGCTCCCAG 
CCTGACCTGT TCGGTGAATT TCTTCATTCG GAATCTCCTG CCGCCGTGCC TGCGTCCTTC 
CCGTCCACCC ACAGCGCCCC ACCGCCCGCC TGCAGCACCG ACTTCCTGCA CCTGGGAGAT 
CTACCAGCAG AGCCTGGCAA GATGACGGCC TCCACCAGCC ACCCGGATCT GCTGGGGGGG 
TGGGAGGCCT GGGCCGAGGC GCCGGCCCCC GCAGCTGCTG CAGAAGGTCC CTTCTTCTCT 
GCAGGAGGCC AGCCTGCCGC CCCCAGTGCC CCCGGGAGCT GGACCAAGTC TCAGAGCCTG 
GACCCGTTTG CTGACCTCGG TGACCTCAGT TCTGGCCTCC AAGGCTCACC GGCTGGATTT 
GCTCCAGGGG CTTTTGGCGC CAAGACGGCC CCCCCTCCCA CGGGCGGCAG CTCCTGGCAG 
CAGACGGGTC GGCCCCCGGC CCAGGGCACC CCGTGGCCCC CCCAGGCCAA GCCGCCCCCC 
AAGGCCTGTC CGCAGCCAAG GCCTAACTAT GCTGCCAACG TGAGCGTGAT TGGCGGCCGA 
GAGGAGAGAG GGGTCCGGGC CCCCAGCTTC GGCCAGAAGC CGAGAGTGTC CGAGAGCGAC 
TTTGAGGACC TGCTGTCCCA CCAGGGCTTC TCGTCCAAGG CTGACCGGAA GGGGCCAAGG 
ACCATGGCTG AGATGCGGCG GCAGGAGCAG GCGAGGGACG CAGACCCGCT GAAGCTGAAG 
CTCCTGGAGT GGACAGAGGG CAAGGAGAGG AACATCCGCG CGCTGCTGTC GACCCTGCAC 
ACCGTGCTGT GGGACGGCGA GAGCCGCTGG GCCCCCGTGG GCATGGCCGA CCTGGTGACC 
CCCGGGCAGG TGAAGAAGCA GTACCGCCGC GCCGTGCTGG TGGTGCACCC GGACAAGGCC 
GCAGGGCAGC CCTACGAGCA GTATGCCAAG ATGATCTTCA TGGAGCTGAA CGACGCGTGG 
GCCGAGTTTG AGAGCCAGGG CTCCCGGCCG CTCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAK ( XM_022417289.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022272997.1 Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X9, mRNA.

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CloneID	OCe287421
Clone ID Related Accession (Same CDS sequence)	XM_022417289.1
Accession Version	XM_022417289.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	cyclin-G-associated kinase isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGTTT CCCCCAAGGC CGGGGTGAAG AGCGCAGGTT TGGGTGCGAT CCGGTACGCA 
GAGGCGGGGG CCGCTGACGC CCGGATGCTG TCGGCCGCCC CGGGGCGCTG CAGCCGTCGC 
CGCCGTCGGG GCCTGTGCTC CGACAACACC GAACACTTGT GCCGGGTTTT TAATACGAGA 
TTCACGGTGG ACAGAGGGTT TGCATTTGTG TATGAAGCTC AAGATCTGGC AAGTGGCAGA 
GAATATGCCT TAAAGGTGGA AAACTTATTG CTCAGTAACC AAGGGACCAT CAAGCTGTGT 
GACTTTGGCA GTGCCACGAC CATATCACAT TACCCCGACT ACAGCTGGAG CGCCCAGAGG 
CGGGCCCTGG TGGAGGAAGA GATCACCAGG AACACCACAC CCATGTACAG GACACCGGAA 
ATCGTGGACT TGTACTCCAA CTTCCCCATC GGCGAGAAGC AGGACATCTG GGCGCTGGGC 
TGCATCTTGT ATCTGCTGTG CTTCCGGCAG CATCCTTTTG AAGACGGAGC AAAGCTTCGG 
ATAGTCAACG GGAAGTTCTC GATCCCCCCC AATGACACCC GGTACTCTGT GTTCCACGAC 
CTCATCCGCG CCACGCTGAA GGTCAACCCC GAGGAGCGGC TGTCCATCAC AGAACTGGTG 
AACCAGCTCC AGGAGATCGC GGCCGCCAGG AACGTGAACC CCAAGTCTCC CATCACTGAG 
CTCCTAGAGC AGAACGGAGG CTATGGGAAC GCTGTCCCTT CCCGAGGGCC ACCCCCTCCT 
GGGGGCTCCA GCAGCGGCTA CAGCGGAGGC CTGGCGCTGG CCGAGTACGA CCAGCCCTAC 
GGCGGGCTCC TGGACATCCT GCGGGGGGGC ACGGAGCGCC TCTTCACAAA CCTCAAGGAC 
ACCTCCTCCA AGGTCATCCA GTCTGTTGCC AATTACGCAA AAGGAGATTT GGACATCTCT 
TACATCACAT CTAGAATTGC TGTGATGTCG TTCCCAGCAG AAGGCGTGGA GTCGGCCATC 
AAGAACAACA TCGAAGACGT GCGGTTGTTT CTGGACTCCA AGCACCCGGG ACATTACGCC 
GTCTACAACC TGTCCCCAAG AACATACCGG CCCTCCAAAT TTCACAACCG GGTCTCCGAG 
TGTGGCTGGG CGGCCAGACG GGCCCCGAAC CTTCGCAGCC TGTACAGTGT GTGTCGGAAC 
ATGCACTCGT GGCTCCGGCA GGACCACAAG AACGTCTGTG TCGTGCACTG CATGGACGGG 
AGAGCTGCGT CTGCGGTGGC CGTGTGCTCC TTCCTGTGCT TCTGCCGCCT GTTCAGCACC 
GCGGAGGCTG CCGTGTACAT GTTCAGCATG AAGCGCTGCC CGCCAGGCAT TTGGCCATCC 
CACAAGAGGT ACATCGAGTA CATGTGTGAC ATGGTGGCCG AGGAGCCCAT CACACCCCAT 
AGCAAGCCCA TCCTGGTGAA AGCCGTGGTC ATGACACCCG TGCCCCTGTT CAGCAAGCAG 
AGAAACGGCT GCAGGCCCTT CTGCGAGGTC TACGTGGGGG ATGAGCGCGT GACTACCACG 
TCTCAGGAGT ACGACAAGAT GAGGGATTTC AGAATCGAGG ATGGCAAAGC AGTCATTCCC 
CTGGGCATAA CAGTGCAAGG GGACGTGCTC ATTGTCATCT ACCACGCCAG GTCCACCCTG 
GGAGGAAGAC TGCAGGCCAA GGTGGCATCC ATGAAGATGT TCCAGGTCCA GTTCCACACG 
GGGTTTGTGC CTCGGAATGC GACTACTGTG AAGTTTGCAA AGTATGACCT TGATGCGTGT 
GACATTCAGG AGAAATACCC AGATTTATTC CAAGTGAACG TGGAGGTGGA GGTGGAGCCC 
AGAGACAGGC CCAGCCGGGA GGCCCCACCC TGGGAGAGCA CCAGCATGAG GGGGCTCAAC 
CCCAAAATCC TGTTTTCCAG CCGAGAGGAG CAGCAAGACA TTCTGTCTAA ATTTGGGAAG 
CCGGAGCTCC CCAGGCAGCC CGGCTCCACC GCTCAGTACG ACGCCGAGGC AGGGTCCCTG 
GACGCAGAGC CCACAGAGTC TGATTCACCA CAGAGCAGCG GCACTGACAC CAGCCACTTC 
CTCCACACGC TGGATTGGCA CGAGGAGAAA GATACAGAGA CGAGTCCAGA AAGTCACTTT 
GCCAAGGAGA ACCTATCAAC CCTGGCCGAG GATGCAGGTG GGAGTGAGCC TTCGGACGAG 
GAAGCAGCCA CACCATCCAC CGAGAGCAGG GATGCTGCGG ATGAGGACAC ACCAGGGCCA 
GCGGCGAGGC TGCCAGAGCG AGATTTGATT TTTGATGGGG CCACGCCGGC CACACCGCAG 
GAGCCCACAC GGCAGGAGGA CAGTGTCGAC CTCCTAGGGC TGCATGCCGA GGCTGGGCTG 
GCCCCCCCTG TGCAGGCCAG TGGGGCAGCC CCCAGCACCG CCGACCTGCT CAGCCGCCTC 
CTGGGGGGCC CTGACGCTGC TCCGGAGGTG CCCCCGGGTG ACCTGCTGGG TGGAGAGGCT 
CCCCTCCTCT TCGCAAGCCC AGCCCCCTCC TCGAGCGCAC AGAGCCCGCC CCGTGAGGGG 
CCAGCTGCCG ACCCCTTTGA CCCTCTCCTG CTGCCATCTG GGCCCAACAC CCAGCCGTGC 
TCCCAGCCTG ACCTGTTCGG TGAATTTCTT CATTCGGAAT CTCCTGCCGC CGTGCCTGCG 
TCCTTCCCGT CCACCCACAG CGCCCCACCG CCCGCCTGCA GCACCGACTT CCTGCACCTG 
GGAGATCTAC CAGCAGAGCC TGGCAAGATG ACGGCCTCCA CCAGCCACCC GGATCTGCTG 
GGGGGGTGGG AGGCCTGGGC CGAGGCGCCG GCCCCCGCAG CTGCTGCAGA AGGTCCCTTC 
TTCTCTGCAG GAGGCCAGCC TGCCGCCCCC AGTGCCCCCG GGAGCTGGAC CAAGTCTCAG 
AGCCTGGACC CGTTTGCTGA CCTCGGTGAC CTCAGTTCTG GCCTCCAAGG CTCACCGGCT 
GGATTTGCTC CAGGGGCTTT TGGCGCCAAG ACGGCCCCCC CTCCCACGGG CGGCAGCTCC 
TGGCAGCAGA CGGGTCGGCC CCCGGCCCAG GGCACCCCGT GGCCCCCCCA GGCCAAGCCG 
CCCCCCAAGG CCTGTCCGCA GCCAAGGCCT AACTATGCTG CCAACGTGAG CGTGATTGGC 
GGCCGAGAGG AGAGAGGGGT CCGGGCCCCC AGCTTCGGCC AGAAGCCGAG AGTGTCCGAG 
AGCGACTTTG AGGACCTGCT GTCCCACCAG GGCTTCTCGT CCAAGGCTGA CCGGAAGGGG 
CCAAGGACCA TGGCTGAGAT GCGGCGGCAG GAGCAGGCGA GGGACGCAGA CCCGCTGAAG 
CTGAAGCTCC TGGAGTGGAC AGAGGGCAAG GAGAGGAACA TCCGCGCGCT GCTGTCGACC 
CTGCACACCG TGCTGTGGGA CGGCGAGAGC CGCTGGGCCC CCGTGGGCAT GGCCGACCTG 
GTGACCCCCG GGCAGGTGAA GAAGCAGTAC CGCCGCGCCG TGCTGGTGGT GCACCCGGAC 
AAGGCCGCAG GGCAGCCCTA CGAGCAGTAT GCCAAGATGA TCTTCATGGA GCTGAACGAC 
GCGTGGGCCG AGTTTGAGAG CCAGGGCTCC CGGCCGCTCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022272997.1
CDS	16..3660
Translation	MRVSPKAGVKSAGLGAIRYAEAGAADARMLSAAPGRCSRRRRRGLCSDNTEHLCRVFNTRFTVDRGFAFVYEAQDLASGREYALKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKFSIPPNDTRYSVFHDLIRATLKVNPEERLSITELVNQLQEIAAARNVNPKSPITELLEQNGGYGNAVPSRGPPPPGGSSSGYSGGLALAEYDQPYGGLLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRLFLDSKHPGHYAVYNLSPRTYRPSKFHNRVSECGWAARRAPNLRSLYSVCRNMHSWLRQDHKNVCVVHCMDGRAASAVAVCSFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYMCDMVAEEPITPHSKPILVKAVVMTPVPLFSKQRNGCRPFCEVYVGDERVTTTSQEYDKMRDFRIEDGKAVIPLGITVQGDVLIVIYHARSTLGGRLQAKVASMKMFQVQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNVEVEVEPRDRPSREAPPWESTSMRGLNPKILFSSREEQQDILSKFGKPELPRQPGSTAQYDAEAGSLDAEPTESDSPQSSGTDTSHFLHTLDWHEEKDTETSPESHFAKENLSTLAEDAGGSEPSDEEAATPSTESRDAADEDTPGPAARLPERDLIFDGATPATPQEPTRQEDSVDLLGLHAEAGLAPPVQASGAAPSTADLLSRLLGGPDAAPEVPPGDLLGGEAPLLFASPAPSSSAQSPPREGPAADPFDPLLLPSGPNTQPCSQPDLFGEFLHSESPAAVPASFPSTHSAPPPACSTDFLHLGDLPAEPGKMTASTSHPDLLGGWEAWAEAPAPAAAAEGPFFSAGGQPAAPSAPGSWTKSQSLDPFADLGDLSSGLQGSPAGFAPGAFGAKTAPPPTGGSSWQQTGRPPAQGTPWPPQAKPPPKACPQPRPNYAANVSVIGGREERGVRAPSFGQKPRVSESDFEDLLSHQGFSSKADRKGPRTMAEMRRQEQARDADPLKLKLLEWTEGKERNIRALLSTLHTVLWDGESRWAPVGMADLVTPGQVKKQYRRAVLVVHPDKAAGQPYEQYAKMIFMELNDAWAEFESQGSRPLF

Target ORF information:

RefSeq Version	XM_022417289.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022417289.1

ORF Insert Sequence:

ATGCGTGTTT CCCCCAAGGC CGGGGTGAAG AGCGCAGGTT TGGGTGCGAT CCGGTACGCA 
GAGGCGGGGG CCGCTGACGC CCGGATGCTG TCGGCCGCCC CGGGGCGCTG CAGCCGTCGC 
CGCCGTCGGG GCCTGTGCTC CGACAACACC GAACACTTGT GCCGGGTTTT TAATACGAGA 
TTCACGGTGG ACAGAGGGTT TGCATTTGTG TATGAAGCTC AAGATCTGGC AAGTGGCAGA 
GAATATGCCT TAAAGGTGGA AAACTTATTG CTCAGTAACC AAGGGACCAT CAAGCTGTGT 
GACTTTGGCA GTGCCACGAC CATATCACAT TACCCCGACT ACAGCTGGAG CGCCCAGAGG 
CGGGCCCTGG TGGAGGAAGA GATCACCAGG AACACCACAC CCATGTACAG GACACCGGAA 
ATCGTGGACT TGTACTCCAA CTTCCCCATC GGCGAGAAGC AGGACATCTG GGCGCTGGGC 
TGCATCTTGT ATCTGCTGTG CTTCCGGCAG CATCCTTTTG AAGACGGAGC AAAGCTTCGG 
ATAGTCAACG GGAAGTTCTC GATCCCCCCC AATGACACCC GGTACTCTGT GTTCCACGAC 
CTCATCCGCG CCACGCTGAA GGTCAACCCC GAGGAGCGGC TGTCCATCAC AGAACTGGTG 
AACCAGCTCC AGGAGATCGC GGCCGCCAGG AACGTGAACC CCAAGTCTCC CATCACTGAG 
CTCCTAGAGC AGAACGGAGG CTATGGGAAC GCTGTCCCTT CCCGAGGGCC ACCCCCTCCT 
GGGGGCTCCA GCAGCGGCTA CAGCGGAGGC CTGGCGCTGG CCGAGTACGA CCAGCCCTAC 
GGCGGGCTCC TGGACATCCT GCGGGGGGGC ACGGAGCGCC TCTTCACAAA CCTCAAGGAC 
ACCTCCTCCA AGGTCATCCA GTCTGTTGCC AATTACGCAA AAGGAGATTT GGACATCTCT 
TACATCACAT CTAGAATTGC TGTGATGTCG TTCCCAGCAG AAGGCGTGGA GTCGGCCATC 
AAGAACAACA TCGAAGACGT GCGGTTGTTT CTGGACTCCA AGCACCCGGG ACATTACGCC 
GTCTACAACC TGTCCCCAAG AACATACCGG CCCTCCAAAT TTCACAACCG GGTCTCCGAG 
TGTGGCTGGG CGGCCAGACG GGCCCCGAAC CTTCGCAGCC TGTACAGTGT GTGTCGGAAC 
ATGCACTCGT GGCTCCGGCA GGACCACAAG AACGTCTGTG TCGTGCACTG CATGGACGGG 
AGAGCTGCGT CTGCGGTGGC CGTGTGCTCC TTCCTGTGCT TCTGCCGCCT GTTCAGCACC 
GCGGAGGCTG CCGTGTACAT GTTCAGCATG AAGCGCTGCC CGCCAGGCAT TTGGCCATCC 
CACAAGAGGT ACATCGAGTA CATGTGTGAC ATGGTGGCCG AGGAGCCCAT CACACCCCAT 
AGCAAGCCCA TCCTGGTGAA AGCCGTGGTC ATGACACCCG TGCCCCTGTT CAGCAAGCAG 
AGAAACGGCT GCAGGCCCTT CTGCGAGGTC TACGTGGGGG ATGAGCGCGT GACTACCACG 
TCTCAGGAGT ACGACAAGAT GAGGGATTTC AGAATCGAGG ATGGCAAAGC AGTCATTCCC 
CTGGGCATAA CAGTGCAAGG GGACGTGCTC ATTGTCATCT ACCACGCCAG GTCCACCCTG 
GGAGGAAGAC TGCAGGCCAA GGTGGCATCC ATGAAGATGT TCCAGGTCCA GTTCCACACG 
GGGTTTGTGC CTCGGAATGC GACTACTGTG AAGTTTGCAA AGTATGACCT TGATGCGTGT 
GACATTCAGG AGAAATACCC AGATTTATTC CAAGTGAACG TGGAGGTGGA GGTGGAGCCC 
AGAGACAGGC CCAGCCGGGA GGCCCCACCC TGGGAGAGCA CCAGCATGAG GGGGCTCAAC 
CCCAAAATCC TGTTTTCCAG CCGAGAGGAG CAGCAAGACA TTCTGTCTAA ATTTGGGAAG 
CCGGAGCTCC CCAGGCAGCC CGGCTCCACC GCTCAGTACG ACGCCGAGGC AGGGTCCCTG 
GACGCAGAGC CCACAGAGTC TGATTCACCA CAGAGCAGCG GCACTGACAC CAGCCACTTC 
CTCCACACGC TGGATTGGCA CGAGGAGAAA GATACAGAGA CGAGTCCAGA AAGTCACTTT 
GCCAAGGAGA ACCTATCAAC CCTGGCCGAG GATGCAGGTG GGAGTGAGCC TTCGGACGAG 
GAAGCAGCCA CACCATCCAC CGAGAGCAGG GATGCTGCGG ATGAGGACAC ACCAGGGCCA 
GCGGCGAGGC TGCCAGAGCG AGATTTGATT TTTGATGGGG CCACGCCGGC CACACCGCAG 
GAGCCCACAC GGCAGGAGGA CAGTGTCGAC CTCCTAGGGC TGCATGCCGA GGCTGGGCTG 
GCCCCCCCTG TGCAGGCCAG TGGGGCAGCC CCCAGCACCG CCGACCTGCT CAGCCGCCTC 
CTGGGGGGCC CTGACGCTGC TCCGGAGGTG CCCCCGGGTG ACCTGCTGGG TGGAGAGGCT 
CCCCTCCTCT TCGCAAGCCC AGCCCCCTCC TCGAGCGCAC AGAGCCCGCC CCGTGAGGGG 
CCAGCTGCCG ACCCCTTTGA CCCTCTCCTG CTGCCATCTG GGCCCAACAC CCAGCCGTGC 
TCCCAGCCTG ACCTGTTCGG TGAATTTCTT CATTCGGAAT CTCCTGCCGC CGTGCCTGCG 
TCCTTCCCGT CCACCCACAG CGCCCCACCG CCCGCCTGCA GCACCGACTT CCTGCACCTG 
GGAGATCTAC CAGCAGAGCC TGGCAAGATG ACGGCCTCCA CCAGCCACCC GGATCTGCTG 
GGGGGGTGGG AGGCCTGGGC CGAGGCGCCG GCCCCCGCAG CTGCTGCAGA AGGTCCCTTC 
TTCTCTGCAG GAGGCCAGCC TGCCGCCCCC AGTGCCCCCG GGAGCTGGAC CAAGTCTCAG 
AGCCTGGACC CGTTTGCTGA CCTCGGTGAC CTCAGTTCTG GCCTCCAAGG CTCACCGGCT 
GGATTTGCTC CAGGGGCTTT TGGCGCCAAG ACGGCCCCCC CTCCCACGGG CGGCAGCTCC 
TGGCAGCAGA CGGGTCGGCC CCCGGCCCAG GGCACCCCGT GGCCCCCCCA GGCCAAGCCG 
CCCCCCAAGG CCTGTCCGCA GCCAAGGCCT AACTATGCTG CCAACGTGAG CGTGATTGGC 
GGCCGAGAGG AGAGAGGGGT CCGGGCCCCC AGCTTCGGCC AGAAGCCGAG AGTGTCCGAG 
AGCGACTTTG AGGACCTGCT GTCCCACCAG GGCTTCTCGT CCAAGGCTGA CCGGAAGGGG 
CCAAGGACCA TGGCTGAGAT GCGGCGGCAG GAGCAGGCGA GGGACGCAGA CCCGCTGAAG 
CTGAAGCTCC TGGAGTGGAC AGAGGGCAAG GAGAGGAACA TCCGCGCGCT GCTGTCGACC 
CTGCACACCG TGCTGTGGGA CGGCGAGAGC CGCTGGGCCC CCGTGGGCAT GGCCGACCTG 
GTGACCCCCG GGCAGGTGAA GAAGCAGTAC CGCCGCGCCG TGCTGGTGGT GCACCCGGAC 
AAGGCCGCAG GGCAGCCCTA CGAGCAGTAT GCCAAGATGA TCTTCATGGA GCTGAACGAC 
GCGTGGGCCG AGTTTGAGAG CCAGGGCTCC CGGCCGCTCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAK ( XM_022417290.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022272998.1 Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X10, mRNA.

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CloneID	OCe287422
Clone ID Related Accession (Same CDS sequence)	XM_022417290.1
Accession Version	XM_022417290.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	cyclin-G-associated kinase isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTGC TGCAGTCTGC GCTGGACTTC CTGGCGGGCC CGGGCTCCCT GGGCGGCGCC 
GCCGGCCGCG ACCAGAGCGA CTTCGTGGGG CAGACGGTGG AGCTGGGCGA GCTGCGGCTG 
CGGGTGCGGC GGGTCCTGGC GGAGGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATCTG 
GCAAGTGGCA GAGAATATGC CTTAAAGGTG GAAAACTTAT TGCTCAGTAA CCAAGGGACC 
ATCAAGCTGT GTGACTTTGG CAGTGCCACG ACCATATCAC ATTACCCCGA CTACAGCTGG 
AGCGCCCAGA GGCGGGCCCT GGTGGAGGAA GAGATCACCA GGAACACCAC ACCCATGTAC 
AGGACACCGG AAATCGTGGA CTTGTACTCC AACTTCCCCA TCGGCGAGAA GCAGGACATC 
TGGGCGCTGG GCTGCATCTT GTATCTGCTG TGCTTCCGGC AGCATCCTTT TGAAGACGGA 
GCAAAGCTTC GGATAGTCAA CGGGAAGTTC TCGATCCCCC CCAATGACAC CCGGTACTCT 
GTGTTCCACG ACCTCATCCG CGCCACGCTG AAGGTCAACC CCGAGGAGCG GCTGTCCATC 
ACAGAACTGG TGAACCAGCT CCAGGAGATC GCGGCCGCCA GGAACGTGAA CCCCAAGTCT 
CCCATCACTG AGCTCCTAGA GCAGAACGGA GGCTATGGGA ACGCTGTCCC TTCCCGAGGG 
CCACCCCCTC CTGGGGGCTC CAGCAGCGGC TACAGCGGAG GCCTGGCGCT GGCCGAGTAC 
GACCAGCCCT ACGGCGGGCT CCTGGACATC CTGCGGGGGG GCACGGAGCG CCTCTTCACA 
AACCTCAAGG ACACCTCCTC CAAGGTCATC CAGTCTGTTG CCAATTACGC AAAAGGAGAT 
TTGGACATCT CTTACATCAC ATCTAGAATT GCTGTGATGT CGTTCCCAGC AGAAGGCGTG 
GAGTCGGCCA TCAAGAACAA CATCGAAGAC GTGCGGTTGT TTCTGGACTC CAAGCACCCG 
GGACATTACG CCGTCTACAA CCTGTCCCCA AGAACATACC GGCCCTCCAA ATTTCACAAC 
CGGGTCTCCG AGTGTGGCTG GGCGGCCAGA CGGGCCCCGA ACCTTCGCAG CCTGTACAGT 
GTGTGTCGGA ACATGCACTC GTGGCTCCGG CAGGACCACA AGAACGTCTG TGTCGTGCAC 
TGCATGGACG GGAGAGCTGC GTCTGCGGTG GCCGTGTGCT CCTTCCTGTG CTTCTGCCGC 
CTGTTCAGCA CCGCGGAGGC TGCCGTGTAC ATGTTCAGCA TGAAGCGCTG CCCGCCAGGC 
ATTTGGCCAT CCCACAAGAG GTACATCGAG TACATGTGTG ACATGGTGGC CGAGGAGCCC 
ATCACACCCC ATAGCAAGCC CATCCTGGTG AAAGCCGTGG TCATGACACC CGTGCCCCTG 
TTCAGCAAGC AGAGAAACGG CTGCAGGCCC TTCTGCGAGG TCTACGTGGG GGATGAGCGC 
GTGACTACCA CGTCTCAGGA GTACGACAAG ATGAGGGATT TCAGAATCGA GGATGGCAAA 
GCAGTCATTC CCCTGGGCAT AACAGTGCAA GGGGACGTGC TCATTGTCAT CTACCACGCC 
AGGTCCACCC TGGGAGGAAG ACTGCAGGCC AAGGTGGCAT CCATGAAGAT GTTCCAGGTC 
CAGTTCCACA CGGGGTTTGT GCCTCGGAAT GCGACTACTG TGAAGTTTGC AAAGTATGAC 
CTTGATGCGT GTGACATTCA GGAGAAATAC CCAGATTTAT TCCAAGTGAA CGTGGAGGTG 
GAGGTGGAGC CCAGAGACAG GCCCAGCCGG GAGGCCCCAC CCTGGGAGAG CACCAGCATG 
AGGGGGCTCA ACCCCAAAAT CCTGTTTTCC AGCCGAGAGG AGCAGCAAGA CATTCTGTCT 
AAATTTGGGA AGCCGGAGCT CCCCAGGCAG CCCGGCTCCA CCGCTCAGTA CGACGCCGAG 
GCAGGGTCCC TGGACGCAGA GCCCACAGAG TCTGATTCAC CACAGAGCAG CGGCACTGAC 
ACCAGCCACT TCCTCCACAC GCTGGATTGG CACGAGGAGA AAGATACAGA GACGAGTCCA 
GAAAGTCACT TTGCCAAGGA GAACCTATCA ACCCTGGCCG AGGATGCAGG TGGGAGTGAG 
CCTTCGGACG AGGAAGCAGC CACACCATCC ACCGAGAGCA GGGATGCTGC GGATGAGGAC 
ACACCAGGGC CAGCGGCGAG GCTGCCAGAG CGAGATTTGA TTTTTGATGG GGCCACGCCG 
GCCACACCGC AGGAGCCCAC ACGGCAGGAG GACAGTGTCG ACCTCCTAGG GCTGCATGCC 
GAGGCTGGGC TGGCCCCCCC TGTGCAGGCC AGTGGGGCAG CCCCCAGCAC CGCCGACCTG 
CTCAGCCGCC TCCTGGGGGG CCCTGACGCT GCTCCGGAGG TGCCCCCGGG TGACCTGCTG 
GGTGGAGAGG CTCCCCTCCT CTTCGCAAGC CCAGCCCCCT CCTCGAGCGC ACAGAGCCCG 
CCCCGTGAGG GGCCAGCTGC CGACCCCTTT GACCCTCTCC TGCTGCCATC TGGGCCCAAC 
ACCCAGCCGT GCTCCCAGCC TGACCTGTTC GGTGAATTTC TTCATTCGGA ATCTCCTGCC 
GCCGTGCCTG CGTCCTTCCC GTCCACCCAC AGCGCCCCAC CGCCCGCCTG CAGCACCGAC 
TTCCTGCACC TGGGAGATCT ACCAGCAGAG CCTGGCAAGA TGACGGCCTC CACCAGCCAC 
CCGGATCTGC TGGGGGGGTG GGAGGCCTGG GCCGAGGCGC CGGCCCCCGC AGCTGCTGCA 
GAAGGTCCCT TCTTCTCTGC AGGAGGCCAG CCTGCCGCCC CCAGTGCCCC CGGGAGCTGG 
ACCAAGTCTC AGAGCCTGGA CCCGTTTGCT GACCTCGGTG ACCTCAGTTC TGGCCTCCAA 
GGCTCACCGG CTGGATTTGC TCCAGGGGCT TTTGGCGCCA AGACGGCCCC CCCTCCCACG 
GGCGGCAGCT CCTGGCAGCA GACGGGTCGG CCCCCGGCCC AGGGCACCCC GTGGCCCCCC 
CAGGCCAAGC CGCCCCCCAA GGCCTGTCCG CAGCCAAGGC CTAACTATGC TGCCAACGTG 
AGCGTGATTG GCGGCCGAGA GGAGAGAGGG GTCCGGGCCC CCAGCTTCGG CCAGAAGCCG 
AGAGTGTCCG AGAGCGACTT TGAGGACCTG CTGTCCCACC AGGGCTTCTC GTCCAAGGCT 
GACCGGAAGG GGCCAAGGAC CATGGCTGAG ATGCGGCGGC AGGAGCAGGC GAGGGACGCA 
GACCCGCTGA AGCTGAAGCT CCTGGAGTGG ACAGAGGGCA AGGAGAGGAA CATCCGCGCG 
CTGCTGTCGA CCCTGCACAC CGTGCTGTGG GACGGCGAGA GCCGCTGGGC CCCCGTGGGC 
ATGGCCGACC TGGTGACCCC CGGGCAGGTG AAGAAGCAGT ACCGCCGCGC CGTGCTGGTG 
GTGCACCCGG ACAAGGCCGC AGGGCAGCCC TACGAGCAGT ATGCCAAGAT GATCTTCATG 
GAGCTGAACG ACGCGTGGGC CGAGTTTGAG AGCCAGGGCT CCCGGCCGCT CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022272998.1
CDS	119..3715
Translation	MSLLQSALDFLAGPGSLGGAAGRDQSDFVGQTVELGELRLRVRRVLAEGGFAFVYEAQDLASGREYALKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKFSIPPNDTRYSVFHDLIRATLKVNPEERLSITELVNQLQEIAAARNVNPKSPITELLEQNGGYGNAVPSRGPPPPGGSSSGYSGGLALAEYDQPYGGLLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRLFLDSKHPGHYAVYNLSPRTYRPSKFHNRVSECGWAARRAPNLRSLYSVCRNMHSWLRQDHKNVCVVHCMDGRAASAVAVCSFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYMCDMVAEEPITPHSKPILVKAVVMTPVPLFSKQRNGCRPFCEVYVGDERVTTTSQEYDKMRDFRIEDGKAVIPLGITVQGDVLIVIYHARSTLGGRLQAKVASMKMFQVQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNVEVEVEPRDRPSREAPPWESTSMRGLNPKILFSSREEQQDILSKFGKPELPRQPGSTAQYDAEAGSLDAEPTESDSPQSSGTDTSHFLHTLDWHEEKDTETSPESHFAKENLSTLAEDAGGSEPSDEEAATPSTESRDAADEDTPGPAARLPERDLIFDGATPATPQEPTRQEDSVDLLGLHAEAGLAPPVQASGAAPSTADLLSRLLGGPDAAPEVPPGDLLGGEAPLLFASPAPSSSAQSPPREGPAADPFDPLLLPSGPNTQPCSQPDLFGEFLHSESPAAVPASFPSTHSAPPPACSTDFLHLGDLPAEPGKMTASTSHPDLLGGWEAWAEAPAPAAAAEGPFFSAGGQPAAPSAPGSWTKSQSLDPFADLGDLSSGLQGSPAGFAPGAFGAKTAPPPTGGSSWQQTGRPPAQGTPWPPQAKPPPKACPQPRPNYAANVSVIGGREERGVRAPSFGQKPRVSESDFEDLLSHQGFSSKADRKGPRTMAEMRRQEQARDADPLKLKLLEWTEGKERNIRALLSTLHTVLWDGESRWAPVGMADLVTPGQVKKQYRRAVLVVHPDKAAGQPYEQYAKMIFMELNDAWAEFESQGSRPLF

Target ORF information:

RefSeq Version	XM_022417290.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022417290.1

ORF Insert Sequence:

ATGTCGCTGC TGCAGTCTGC GCTGGACTTC CTGGCGGGCC CGGGCTCCCT GGGCGGCGCC 
GCCGGCCGCG ACCAGAGCGA CTTCGTGGGG CAGACGGTGG AGCTGGGCGA GCTGCGGCTG 
CGGGTGCGGC GGGTCCTGGC GGAGGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATCTG 
GCAAGTGGCA GAGAATATGC CTTAAAGGTG GAAAACTTAT TGCTCAGTAA CCAAGGGACC 
ATCAAGCTGT GTGACTTTGG CAGTGCCACG ACCATATCAC ATTACCCCGA CTACAGCTGG 
AGCGCCCAGA GGCGGGCCCT GGTGGAGGAA GAGATCACCA GGAACACCAC ACCCATGTAC 
AGGACACCGG AAATCGTGGA CTTGTACTCC AACTTCCCCA TCGGCGAGAA GCAGGACATC 
TGGGCGCTGG GCTGCATCTT GTATCTGCTG TGCTTCCGGC AGCATCCTTT TGAAGACGGA 
GCAAAGCTTC GGATAGTCAA CGGGAAGTTC TCGATCCCCC CCAATGACAC CCGGTACTCT 
GTGTTCCACG ACCTCATCCG CGCCACGCTG AAGGTCAACC CCGAGGAGCG GCTGTCCATC 
ACAGAACTGG TGAACCAGCT CCAGGAGATC GCGGCCGCCA GGAACGTGAA CCCCAAGTCT 
CCCATCACTG AGCTCCTAGA GCAGAACGGA GGCTATGGGA ACGCTGTCCC TTCCCGAGGG 
CCACCCCCTC CTGGGGGCTC CAGCAGCGGC TACAGCGGAG GCCTGGCGCT GGCCGAGTAC 
GACCAGCCCT ACGGCGGGCT CCTGGACATC CTGCGGGGGG GCACGGAGCG CCTCTTCACA 
AACCTCAAGG ACACCTCCTC CAAGGTCATC CAGTCTGTTG CCAATTACGC AAAAGGAGAT 
TTGGACATCT CTTACATCAC ATCTAGAATT GCTGTGATGT CGTTCCCAGC AGAAGGCGTG 
GAGTCGGCCA TCAAGAACAA CATCGAAGAC GTGCGGTTGT TTCTGGACTC CAAGCACCCG 
GGACATTACG CCGTCTACAA CCTGTCCCCA AGAACATACC GGCCCTCCAA ATTTCACAAC 
CGGGTCTCCG AGTGTGGCTG GGCGGCCAGA CGGGCCCCGA ACCTTCGCAG CCTGTACAGT 
GTGTGTCGGA ACATGCACTC GTGGCTCCGG CAGGACCACA AGAACGTCTG TGTCGTGCAC 
TGCATGGACG GGAGAGCTGC GTCTGCGGTG GCCGTGTGCT CCTTCCTGTG CTTCTGCCGC 
CTGTTCAGCA CCGCGGAGGC TGCCGTGTAC ATGTTCAGCA TGAAGCGCTG CCCGCCAGGC 
ATTTGGCCAT CCCACAAGAG GTACATCGAG TACATGTGTG ACATGGTGGC CGAGGAGCCC 
ATCACACCCC ATAGCAAGCC CATCCTGGTG AAAGCCGTGG TCATGACACC CGTGCCCCTG 
TTCAGCAAGC AGAGAAACGG CTGCAGGCCC TTCTGCGAGG TCTACGTGGG GGATGAGCGC 
GTGACTACCA CGTCTCAGGA GTACGACAAG ATGAGGGATT TCAGAATCGA GGATGGCAAA 
GCAGTCATTC CCCTGGGCAT AACAGTGCAA GGGGACGTGC TCATTGTCAT CTACCACGCC 
AGGTCCACCC TGGGAGGAAG ACTGCAGGCC AAGGTGGCAT CCATGAAGAT GTTCCAGGTC 
CAGTTCCACA CGGGGTTTGT GCCTCGGAAT GCGACTACTG TGAAGTTTGC AAAGTATGAC 
CTTGATGCGT GTGACATTCA GGAGAAATAC CCAGATTTAT TCCAAGTGAA CGTGGAGGTG 
GAGGTGGAGC CCAGAGACAG GCCCAGCCGG GAGGCCCCAC CCTGGGAGAG CACCAGCATG 
AGGGGGCTCA ACCCCAAAAT CCTGTTTTCC AGCCGAGAGG AGCAGCAAGA CATTCTGTCT 
AAATTTGGGA AGCCGGAGCT CCCCAGGCAG CCCGGCTCCA CCGCTCAGTA CGACGCCGAG 
GCAGGGTCCC TGGACGCAGA GCCCACAGAG TCTGATTCAC CACAGAGCAG CGGCACTGAC 
ACCAGCCACT TCCTCCACAC GCTGGATTGG CACGAGGAGA AAGATACAGA GACGAGTCCA 
GAAAGTCACT TTGCCAAGGA GAACCTATCA ACCCTGGCCG AGGATGCAGG TGGGAGTGAG 
CCTTCGGACG AGGAAGCAGC CACACCATCC ACCGAGAGCA GGGATGCTGC GGATGAGGAC 
ACACCAGGGC CAGCGGCGAG GCTGCCAGAG CGAGATTTGA TTTTTGATGG GGCCACGCCG 
GCCACACCGC AGGAGCCCAC ACGGCAGGAG GACAGTGTCG ACCTCCTAGG GCTGCATGCC 
GAGGCTGGGC TGGCCCCCCC TGTGCAGGCC AGTGGGGCAG CCCCCAGCAC CGCCGACCTG 
CTCAGCCGCC TCCTGGGGGG CCCTGACGCT GCTCCGGAGG TGCCCCCGGG TGACCTGCTG 
GGTGGAGAGG CTCCCCTCCT CTTCGCAAGC CCAGCCCCCT CCTCGAGCGC ACAGAGCCCG 
CCCCGTGAGG GGCCAGCTGC CGACCCCTTT GACCCTCTCC TGCTGCCATC TGGGCCCAAC 
ACCCAGCCGT GCTCCCAGCC TGACCTGTTC GGTGAATTTC TTCATTCGGA ATCTCCTGCC 
GCCGTGCCTG CGTCCTTCCC GTCCACCCAC AGCGCCCCAC CGCCCGCCTG CAGCACCGAC 
TTCCTGCACC TGGGAGATCT ACCAGCAGAG CCTGGCAAGA TGACGGCCTC CACCAGCCAC 
CCGGATCTGC TGGGGGGGTG GGAGGCCTGG GCCGAGGCGC CGGCCCCCGC AGCTGCTGCA 
GAAGGTCCCT TCTTCTCTGC AGGAGGCCAG CCTGCCGCCC CCAGTGCCCC CGGGAGCTGG 
ACCAAGTCTC AGAGCCTGGA CCCGTTTGCT GACCTCGGTG ACCTCAGTTC TGGCCTCCAA 
GGCTCACCGG CTGGATTTGC TCCAGGGGCT TTTGGCGCCA AGACGGCCCC CCCTCCCACG 
GGCGGCAGCT CCTGGCAGCA GACGGGTCGG CCCCCGGCCC AGGGCACCCC GTGGCCCCCC 
CAGGCCAAGC CGCCCCCCAA GGCCTGTCCG CAGCCAAGGC CTAACTATGC TGCCAACGTG 
AGCGTGATTG GCGGCCGAGA GGAGAGAGGG GTCCGGGCCC CCAGCTTCGG CCAGAAGCCG 
AGAGTGTCCG AGAGCGACTT TGAGGACCTG CTGTCCCACC AGGGCTTCTC GTCCAAGGCT 
GACCGGAAGG GGCCAAGGAC CATGGCTGAG ATGCGGCGGC AGGAGCAGGC GAGGGACGCA 
GACCCGCTGA AGCTGAAGCT CCTGGAGTGG ACAGAGGGCA AGGAGAGGAA CATCCGCGCG 
CTGCTGTCGA CCCTGCACAC CGTGCTGTGG GACGGCGAGA GCCGCTGGGC CCCCGTGGGC 
ATGGCCGACC TGGTGACCCC CGGGCAGGTG AAGAAGCAGT ACCGCCGCGC CGTGCTGGTG 
GTGCACCCGG ACAAGGCCGC AGGGCAGCCC TACGAGCAGT ATGCCAAGAT GATCTTCATG 
GAGCTGAACG ACGCGTGGGC CGAGTTTGAG AGCCAGGGCT CCCGGCCGCT CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAK ( XM_022417291.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022272999.1 Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OCe287423
Clone ID Related Accession (Same CDS sequence)	XM_022417291.1
Accession Version	XM_022417291.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	cyclin-G-associated kinase isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGTTT CCCCCAAGGC CGGGGTGAAG AGCGCAGGTT TGGGTGCGAT CCGGTACGCA 
GAGGCGGGGG CCGCTGACGC CCGGATGCTG TCGGCCGCCC CGGGGCGCTG CAGCCGTCGC 
CGCCGTCGGG GCCTGTGCTC CGACAACACC GAACACTTGT GCCGGGTTTT TAATACGAGA 
TTCACGGTGG ACAGAGGGTT TGCATTTGTG TATGAAGCTC AAGATCTGGC AAGTGGCAGA 
GAATATGCCT TAAAGAGATT ATTGTCTAAC GAAGAGGAAA AGAACAGAGC CATCATTCAG 
GAAGTTTGTT TCATGAAAAA ACTTTCTGGC CACCCCAATA TTGTCCAGTT TTGTTCTGCA 
GCGTCCATAG GGAAAGAGGA GTCGGACACT GGGCAGGCTG AGTTCCTGCT GCTCACCGAG 
CTCTGCAAAG GGCAGCTGGT GGACTTTTTA AAGAAAATTG AATCTAGAGG TCCGCTCTCG 
TGCGATACTG TTCTGAAGAT TTTCTATCAG ACGTGCAGAG CGGTGCAGCA CATGCACAGA 
CAAAAGCCGC CCATCATCCA TAGAGACCTC AAGGTGGAAA ACTTATTGCT CAGTAACCAA 
GGGACCATCA AGCTGTGTGA CTTTGGCAGT GCCACGACCA TATCACATTA CCCCGACTAC 
AGCTGGAGCG CCCAGAGGCG GGCCCTGGTG GAGGAAGAGA TCACCAGGAA CACCACACCC 
ATGTACAGGA CACCGGAAAT CGTGGACTTG TACTCCAACT TCCCCATCGG CGAGAAGCAG 
GACATCTGGG CGCTGGGCTG CATCTTGTAT CTGCTGTGCT TCCGGCAGCA TCCTTTTGAA 
GACGGAGCAA AGCTTCGGAT AGTCAACGGG AAGTTCTCGA TCCCCCCCAA TGACACCCGG 
TACTCTGTGT TCCACGACCT CATCCGCGCC ACGCTGAAGG TCAACCCCGA GGAGCGGCTG 
TCCATCACAG AACTGGTGAA CCAGCTCCAG GAGATCGCGG CCGCCAGGAA CGTGAACCCC 
AAGTCTCCCA TCACTGAGCT CCTAGAGCAG AACGGAGGCT ATGGGAACGC TGTCCCTTCC 
CGAGGGCCAC CCCCTCCTGG GGGCTCCAGC AGCGGCTACA GCGGAGGCCT GGCGCTGGCC 
GAGTACGACC AGCCCTACGG CGGGCTCCTG GACATCCTGC GGGGGGGCAC GGAGCGCCTC 
TTCACAAACC TCAAGGACAC CTCCTCCAAG GTCATCCAGT CTGTTGCCAA TTACGCAAAA 
GGAGATTTGG ACATCTCTTA CATCACATCT AGAATTGCTG TGATGTCGTT CCCAGCAGAA 
GGCGTGGAGT CGGCCATCAA GAACAACATC GAAGACGTGC GGTTGTTTCT GGACTCCAAG 
CACCCGGGAC ATTACGCCGT CTACAACCTG TCCCCAAGAA CATACCGGCC CTCCAAATTT 
CACAACCGGG TCTCCGAGTG TGGCTGGGCG GCCAGACGGG CCCCGAACCT TCGCAGCCTG 
TACAGTGTGT GTCGGAACAT GCACTCGTGG CTCCGGCAGG ACCACAAGAA CGTCTGTGTC 
GTGCACTGCA TGGACGGGAG AGCTGCGTCT GCGGTGGCCG TGTGCTCCTT CCTGTGCTTC 
TGCCGCCTGT TCAGCACCGC GGAGGCTGCC GTGTACATGT TCAGCATGAA GCGCTGCCCG 
CCAGGCATTT GGCCATCCCA CAAGAGGTAC ATCGAGTACA TGTGTGACAT GGTGGCCGAG 
GAGCCCATCA CACCCCATAG CAAGCCCATC CTGGTGAAAG CCGTGGTCAT GACACCCGTG 
CCCCTGTTCA GCAAGCAGAG AAACGGCTGC AGGCCCTTCT GCGAGGTCTA CGTGGGGGAT 
GAGCGCGTGA CTACCACGTC TCAGGAGTAC GACAAGATGA GGGATTTCAG AATCGAGGAT 
GGCAAAGCAG TCATTCCCCT GGGCATAACA GTGCAAGGGG ACGTGCTCAT TGTCATCTAC 
CACGCCAGGT CCACCCTGGG AGGAAGACTG CAGGCCAAGG TGGCATCCAT GAAGATGTTC 
CAGGTCCAGT TCCACACGGG GTTTGTGCCT CGGAATGCGA CTACTGTGAA GTTTGCAAAG 
TATGACCTTG ATGCGTGTGA CATTCAGGAG AAATACCCAG ATTTATTCCA AGTGAACGTG 
GAGGTGGAGG TGGAGCCCAG AGACAGGCCC AGCCGGGAGG CCCCACCCTG GGAGAGCACC 
AGCATGAGGG GGCTCAACCC CAAAATCCTG TTTTCCAGCC GAGAGGAGCA GCAAGACATT 
CTGTCTAAAT TTGGGAAGCC GGAGCTCCCC AGGCAGCCCG GCTCCACCGC TCAGTACGAC 
GCCGAGGCAG GGTCCCTGGA CGCAGAGCCC ACAGAGTCTG ATTCACCACA GAGCAGCGGC 
ACTGACACCA GCCACTTCCT CCACACGCTG GATTGGCACG AGGAGAAAGA TACAGAGACG 
AGTCCAGAAA GTCACTTTGC CAAGGAGAAC CTATCAACCC TGGCCGAGGA TGCAGGTGGG 
AGTGAGCCTT CGGACGAGGA AGCAGCCACA CCATCCACCG AGAGCAGGGA TGCTGCGGAT 
GAGGACACAC CAGGGCCAGC GGCGAGGCTG CCAGAGCGAG ATTTGATTTT TGATGGGGCC 
ACGCCGGCCA CACCGCAGGA GCCCACACGG CAGGAGGACA GTGTCGACCT CCTAGGGCTG 
CATGCCGAGG CTGGGCTGGC CCCCCCTGTG CAGGCCAGTG GGGCAGCCCC CAGCACCGCC 
GACCTGCTCA GCCGCCTCCT GGGGGGCCCT GACGCTGCTC CGGAGGTGCC CCCGGGTGAC 
CTGCTGGGTG GAGAGGCTCC CCTCCTCTTC GCAAGCCCAG CCCCCTCCTC GAGCGCACAG 
AGCCCGCCCC GTGAGGGGCC AGCTGCCGAC CCCTTTGACC CTCTCCTGCT GCCATCTGGG 
CCCAACACCC AGCCGTGCTC CCAGCCTGAC CTGTTCGGTG AATTTCTTCA TTCGGAATCT 
CCTGCCGCCG TGCCTGCGTC CTTCCCGTCC ACCCACAGCG CCCCACCGCC CGCCTGCAGC 
ACCGACTTCC TGCACCTGGG AGATCTACCA GCAGAGCCTG GCAAGATGAC GGCCTCCACC 
AGCCACCCGG ATCTGCTGGG GGGGTGGGAG GCCTGGGCCG AGGCGCCGGC CCCCGCAGCT 
GCTGCAGAAG GTCCCTTCTT CTCTGCAGGA GGCCAGCCTG CCGCCCCCAG TGCCCCCGGG 
AGCTGGACCA AGTCTCAGAG CCTGGACCCG TTTGCTGACC TCGGTGACCT CAGTTCTGGC 
CTCCAAGGTG ATGGAGCAGC ACGTGAGGTT GGCGCAGCGC TGGAGTTGCT GGATGCGATC 
ACGGCTGGCG GGTCCCCATG GCGGTCCCGG CCCTTCTCCC AGGCACTCTG GGCCTTCGTC 
GAGACTGCAT GGACTGGGAA TCAGGAACAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022272999.1
CDS	16..3468
Translation	MRVSPKAGVKSAGLGAIRYAEAGAADARMLSAAPGRCSRRRRRGLCSDNTEHLCRVFNTRFTVDRGFAFVYEAQDLASGREYALKRLLSNEEEKNRAIIQEVCFMKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLTELCKGQLVDFLKKIESRGPLSCDTVLKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKFSIPPNDTRYSVFHDLIRATLKVNPEERLSITELVNQLQEIAAARNVNPKSPITELLEQNGGYGNAVPSRGPPPPGGSSSGYSGGLALAEYDQPYGGLLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRLFLDSKHPGHYAVYNLSPRTYRPSKFHNRVSECGWAARRAPNLRSLYSVCRNMHSWLRQDHKNVCVVHCMDGRAASAVAVCSFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYMCDMVAEEPITPHSKPILVKAVVMTPVPLFSKQRNGCRPFCEVYVGDERVTTTSQEYDKMRDFRIEDGKAVIPLGITVQGDVLIVIYHARSTLGGRLQAKVASMKMFQVQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNVEVEVEPRDRPSREAPPWESTSMRGLNPKILFSSREEQQDILSKFGKPELPRQPGSTAQYDAEAGSLDAEPTESDSPQSSGTDTSHFLHTLDWHEEKDTETSPESHFAKENLSTLAEDAGGSEPSDEEAATPSTESRDAADEDTPGPAARLPERDLIFDGATPATPQEPTRQEDSVDLLGLHAEAGLAPPVQASGAAPSTADLLSRLLGGPDAAPEVPPGDLLGGEAPLLFASPAPSSSAQSPPREGPAADPFDPLLLPSGPNTQPCSQPDLFGEFLHSESPAAVPASFPSTHSAPPPACSTDFLHLGDLPAEPGKMTASTSHPDLLGGWEAWAEAPAPAAAAEGPFFSAGGQPAAPSAPGSWTKSQSLDPFADLGDLSSGLQGDGAAREVGAALELLDAITAGGSPWRSRPFSQALWAFVETAWTGNQEQ

Target ORF information:

RefSeq Version	XM_022417291.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022417291.1

ORF Insert Sequence:

ATGCGTGTTT CCCCCAAGGC CGGGGTGAAG AGCGCAGGTT TGGGTGCGAT CCGGTACGCA 
GAGGCGGGGG CCGCTGACGC CCGGATGCTG TCGGCCGCCC CGGGGCGCTG CAGCCGTCGC 
CGCCGTCGGG GCCTGTGCTC CGACAACACC GAACACTTGT GCCGGGTTTT TAATACGAGA 
TTCACGGTGG ACAGAGGGTT TGCATTTGTG TATGAAGCTC AAGATCTGGC AAGTGGCAGA 
GAATATGCCT TAAAGAGATT ATTGTCTAAC GAAGAGGAAA AGAACAGAGC CATCATTCAG 
GAAGTTTGTT TCATGAAAAA ACTTTCTGGC CACCCCAATA TTGTCCAGTT TTGTTCTGCA 
GCGTCCATAG GGAAAGAGGA GTCGGACACT GGGCAGGCTG AGTTCCTGCT GCTCACCGAG 
CTCTGCAAAG GGCAGCTGGT GGACTTTTTA AAGAAAATTG AATCTAGAGG TCCGCTCTCG 
TGCGATACTG TTCTGAAGAT TTTCTATCAG ACGTGCAGAG CGGTGCAGCA CATGCACAGA 
CAAAAGCCGC CCATCATCCA TAGAGACCTC AAGGTGGAAA ACTTATTGCT CAGTAACCAA 
GGGACCATCA AGCTGTGTGA CTTTGGCAGT GCCACGACCA TATCACATTA CCCCGACTAC 
AGCTGGAGCG CCCAGAGGCG GGCCCTGGTG GAGGAAGAGA TCACCAGGAA CACCACACCC 
ATGTACAGGA CACCGGAAAT CGTGGACTTG TACTCCAACT TCCCCATCGG CGAGAAGCAG 
GACATCTGGG CGCTGGGCTG CATCTTGTAT CTGCTGTGCT TCCGGCAGCA TCCTTTTGAA 
GACGGAGCAA AGCTTCGGAT AGTCAACGGG AAGTTCTCGA TCCCCCCCAA TGACACCCGG 
TACTCTGTGT TCCACGACCT CATCCGCGCC ACGCTGAAGG TCAACCCCGA GGAGCGGCTG 
TCCATCACAG AACTGGTGAA CCAGCTCCAG GAGATCGCGG CCGCCAGGAA CGTGAACCCC 
AAGTCTCCCA TCACTGAGCT CCTAGAGCAG AACGGAGGCT ATGGGAACGC TGTCCCTTCC 
CGAGGGCCAC CCCCTCCTGG GGGCTCCAGC AGCGGCTACA GCGGAGGCCT GGCGCTGGCC 
GAGTACGACC AGCCCTACGG CGGGCTCCTG GACATCCTGC GGGGGGGCAC GGAGCGCCTC 
TTCACAAACC TCAAGGACAC CTCCTCCAAG GTCATCCAGT CTGTTGCCAA TTACGCAAAA 
GGAGATTTGG ACATCTCTTA CATCACATCT AGAATTGCTG TGATGTCGTT CCCAGCAGAA 
GGCGTGGAGT CGGCCATCAA GAACAACATC GAAGACGTGC GGTTGTTTCT GGACTCCAAG 
CACCCGGGAC ATTACGCCGT CTACAACCTG TCCCCAAGAA CATACCGGCC CTCCAAATTT 
CACAACCGGG TCTCCGAGTG TGGCTGGGCG GCCAGACGGG CCCCGAACCT TCGCAGCCTG 
TACAGTGTGT GTCGGAACAT GCACTCGTGG CTCCGGCAGG ACCACAAGAA CGTCTGTGTC 
GTGCACTGCA TGGACGGGAG AGCTGCGTCT GCGGTGGCCG TGTGCTCCTT CCTGTGCTTC 
TGCCGCCTGT TCAGCACCGC GGAGGCTGCC GTGTACATGT TCAGCATGAA GCGCTGCCCG 
CCAGGCATTT GGCCATCCCA CAAGAGGTAC ATCGAGTACA TGTGTGACAT GGTGGCCGAG 
GAGCCCATCA CACCCCATAG CAAGCCCATC CTGGTGAAAG CCGTGGTCAT GACACCCGTG 
CCCCTGTTCA GCAAGCAGAG AAACGGCTGC AGGCCCTTCT GCGAGGTCTA CGTGGGGGAT 
GAGCGCGTGA CTACCACGTC TCAGGAGTAC GACAAGATGA GGGATTTCAG AATCGAGGAT 
GGCAAAGCAG TCATTCCCCT GGGCATAACA GTGCAAGGGG ACGTGCTCAT TGTCATCTAC 
CACGCCAGGT CCACCCTGGG AGGAAGACTG CAGGCCAAGG TGGCATCCAT GAAGATGTTC 
CAGGTCCAGT TCCACACGGG GTTTGTGCCT CGGAATGCGA CTACTGTGAA GTTTGCAAAG 
TATGACCTTG ATGCGTGTGA CATTCAGGAG AAATACCCAG ATTTATTCCA AGTGAACGTG 
GAGGTGGAGG TGGAGCCCAG AGACAGGCCC AGCCGGGAGG CCCCACCCTG GGAGAGCACC 
AGCATGAGGG GGCTCAACCC CAAAATCCTG TTTTCCAGCC GAGAGGAGCA GCAAGACATT 
CTGTCTAAAT TTGGGAAGCC GGAGCTCCCC AGGCAGCCCG GCTCCACCGC TCAGTACGAC 
GCCGAGGCAG GGTCCCTGGA CGCAGAGCCC ACAGAGTCTG ATTCACCACA GAGCAGCGGC 
ACTGACACCA GCCACTTCCT CCACACGCTG GATTGGCACG AGGAGAAAGA TACAGAGACG 
AGTCCAGAAA GTCACTTTGC CAAGGAGAAC CTATCAACCC TGGCCGAGGA TGCAGGTGGG 
AGTGAGCCTT CGGACGAGGA AGCAGCCACA CCATCCACCG AGAGCAGGGA TGCTGCGGAT 
GAGGACACAC CAGGGCCAGC GGCGAGGCTG CCAGAGCGAG ATTTGATTTT TGATGGGGCC 
ACGCCGGCCA CACCGCAGGA GCCCACACGG CAGGAGGACA GTGTCGACCT CCTAGGGCTG 
CATGCCGAGG CTGGGCTGGC CCCCCCTGTG CAGGCCAGTG GGGCAGCCCC CAGCACCGCC 
GACCTGCTCA GCCGCCTCCT GGGGGGCCCT GACGCTGCTC CGGAGGTGCC CCCGGGTGAC 
CTGCTGGGTG GAGAGGCTCC CCTCCTCTTC GCAAGCCCAG CCCCCTCCTC GAGCGCACAG 
AGCCCGCCCC GTGAGGGGCC AGCTGCCGAC CCCTTTGACC CTCTCCTGCT GCCATCTGGG 
CCCAACACCC AGCCGTGCTC CCAGCCTGAC CTGTTCGGTG AATTTCTTCA TTCGGAATCT 
CCTGCCGCCG TGCCTGCGTC CTTCCCGTCC ACCCACAGCG CCCCACCGCC CGCCTGCAGC 
ACCGACTTCC TGCACCTGGG AGATCTACCA GCAGAGCCTG GCAAGATGAC GGCCTCCACC 
AGCCACCCGG ATCTGCTGGG GGGGTGGGAG GCCTGGGCCG AGGCGCCGGC CCCCGCAGCT 
GCTGCAGAAG GTCCCTTCTT CTCTGCAGGA GGCCAGCCTG CCGCCCCCAG TGCCCCCGGG 
AGCTGGACCA AGTCTCAGAG CCTGGACCCG TTTGCTGACC TCGGTGACCT CAGTTCTGGC 
CTCCAAGGTG ATGGAGCAGC ACGTGAGGTT GGCGCAGCGC TGGAGTTGCT GGATGCGATC 
ACGGCTGGCG GGTCCCCATG GCGGTCCCGG CCCTTCTCCC AGGCACTCTG GGCCTTCGTC 
GAGACTGCAT GGACTGGGAA TCAGGAACAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAK ( XM_022417292.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022273000.1 Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X12, mRNA.

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CloneID	OCe287424
Clone ID Related Accession (Same CDS sequence)	XM_022417292.1
Accession Version	XM_022417292.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	cyclin-G-associated kinase isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGTTT CCCCCAAGGC CGGGGTGAAG AGCGCAGGTT TGGGTGCGAT CCGGTACGCA 
GAGGCGGGGG CCGCTGACGC CCGGATGCTG TCGGCCGCCC CGGGGCGCTG CAGCCGTCGC 
CGCCGTCGGG GCCTGTGCTC CGACAACACC GAACACTTGT GCCGGGTTTT TAATACGAGA 
TTCACGGTGG ACAGAGGGTT TGCATTTGTG TATGAAGCTC AAGATCTGGC AAGTGGCAGA 
GAATATGCCT TAAAGAGATT ATTGTCTAAC GAAGAGGAAA AGAACAGAGC CATCATTCAG 
GAAGTTTGTT TCATGAAAAA ACTTTCTGGC CACCCCAATA TTGTCCAGTT TTGTTCTGCA 
GCGTCCATAG GGAAAGAGGA GTCGGACACT GGGCAGGCTG AGTTCCTGCT GCTCACCGAG 
CTCTGCAAAG GGCAGCTGGT GGACTTTTTA AAGAAAATTG AATCTAGAGG TCCGCTCTCG 
TGCGATACTG TTCTGAAGAT TTTCTATCAG ACGTGCAGAG CGGTGCAGCA CATGCACAGA 
CAAAAGCCGC CCATCATCCA TAGAGACCTC AAGGTGGAAA ACTTATTGCT CAGTAACCAA 
GGGACCATCA AGCTGTGTGA CTTTGGCAGT GCCACGACCA TATCACATTA CCCCGACTAC 
AGCTGGAGCG CCCAGAGGCG GGCCCTGGTG GAGGAAGAGA TCACCAGGAA CACCACACCC 
ATGTACAGGA CACCGGAAAT CGTGGACTTG TACTCCAACT TCCCCATCGG CGAGAAGCAG 
GACATCTGGG CGCTGGGCTG CATCTTGTAT CTGCTGTGCT TCCGGCAGCA TCCTTTTGAA 
GACGGAGCAA AGCTTCGGAT AGTCAACGGG AAGTTCTCGA TCCCCCCCAA TGACACCCGG 
TACTCTGTGT TCCACGACCT CATCCGCGCC ACGCTGAAGG TCAACCCCGA GGAGCGGCTG 
TCCATCACAG AACTGGTGAA CCAGCTCCAG GAGATCGCGG CCGCCAGGAA CGTGAACCCC 
AAGTCTCCCA TCACTGAGCT CCTAGAGCAG AACGGAGGCT ATGGGAACGC TGTCCCTTCC 
CGAGGGCCAC CCCCTCCTGG GGGCTCCAGC AGCGGCTACA GCGGAGGCCT GGCGCTGGCC 
GAGTACGACC AGCCCTACGG CGGGCTCCTG GACATCCTGC GGGGGGGCAC GGAGCGCCTC 
TTCACAAACC TCAAGGACAC CTCCTCCAAG GTCATCCAGT CTGTTGCCAA TTACGCAAAA 
GGAGATTTGG ACATCTCTTA CATCACATCT AGAATTGCTG TGATGTCGTT CCCAGCAGAA 
GGCGTGGAGT CGGCCATCAA GAACAACATC GAAGACGTGC GGTTGTTTCT GGACTCCAAG 
CACCCGGGAC ATTACGCCGT CTACAACCTG TCCCCAAGAA CATACCGGCC CTCCAAATTT 
CACAACCGGG TCTCCGAGTG TGGCTGGGCG GCCAGACGGG CCCCGAACCT TCGCAGCCTG 
TACAGTGTGT GTCGGAACAT GCACTCGTGG CTCCGGCAGG ACCACAAGAA CGTCTGTGTC 
GTGCACTGCA TGGACGGGAG AGCTGCGTCT GCGGTGGCCG TGTGCTCCTT CCTGTGCTTC 
TGCCGCCTGT TCAGCACCGC GGAGGCTGCC GTGTACATGT TCAGCATGAA GCGCTGCCCG 
CCAGGCATTT GGCCATCCCA CAAGAGGTAC ATCGAGTACA TGTGTGACAT GGTGGCCGAG 
GAGCCCATCA CACCCCATAG CAAGCCCATC CTGGTGAAAG CCGTGGTCAT GACACCCGTG 
CCCCTGTTCA GCAAGCAGAG AAACGGCTGC AGGCCCTTCT GCGAGGTCTA CGTGGGGGAT 
GAGCGCGTGA CTACCACGTC TCAGGAGTAC GACAAGATGA GGGATTTCAG AATCGAGGAT 
GGCAAAGCAG TCATTCCCCT GGGCATAACA GTGCAAGGGG ACGTGCTCAT TGTCATCTAC 
CACGCCAGGT CCACCCTGGG AGGAAGACTG CAGGCCAAGG TGGCATCCAT GAAGATGTTC 
CAGGTCCAGT TCCACACGGG GTTTGTGCCT CGGAATGCGA CTACTGTGAA GTTTGCAAAG 
TATGACCTTG ATGCGTGTGA CATTCAGGAG AAATACCCAG ATTTATTCCA AGTGAACGTG 
GAGGTGGAGG TGGAGCCCAG AGACAGGCCC AGCCGGGAGG CCCCACCCTG GGAGAGCACC 
AGCATGAGGG GGCTCAACCC CAAAATCCTG TTTTCCAGCC GAGAGGAGCA GCAAGACATT 
CTGTCTAAAT TTGGGAAGCC GGAGCTCCCC AGGCAGCCCG GCTCCACCGC TCAGTACGAC 
GCCGAGGCAG GGTCCCTGGA CGCAGAGCCC ACAGAGTCTG ATTCACCACA GAGCAGCGGC 
ACTGACACCA GCCACTTCCT CCACACGCTG GATTGGCACG AGGAGAAAGA TACAGAGACG 
AGTCCAGAAA GTCACTTTGC CAAGGAGAAC CTATCAACCC TGGCCGAGGA TGCAGGTGGG 
AGTGAGCCTT CGGACGAGGA AGCAGCCACA CCATCCACCG AGAGCAGGGA TGCTGCGGAT 
GAGGACACAC CAGGGCCAGC GGCGAGGCTG CCAGAGCGAG ATTTGATTTT TGATGGGGCC 
ACGCCGGCCA CACCGCAGGA GCCCACACGG CAGGAGGACA GTGTCGACCT CCTAGGGCTG 
CATGCCGAGG CTGGGCTGGC CCCCCCTGTG CAGGCCAGTG GGGCAGCCCC CAGCACCGCC 
GACCTGCTCA GCCGCCTCCT GGGGGGCCCT GACGCTGCTC CGGAGGTGCC CCCGGGTGAC 
CTGCTGGGTG GAGAGGCTCC CCTCCTCTTC GCAAGCCCAG CCCCCTCCTC GAGCGCACAG 
AGCCCGCCCC GTGAGGGGCC AGCTGCCGAC CCCTTTGACC CTCTCCTGCT GCCATCTGGG 
CCCAACACCC AGCCGTGCTC CCAGCCTGAC CTGTTCGGTG AATTTCTTCA TTCGGAATCT 
CCTGCCGCCG TGCCTGCGTC CTTCCCGTCC ACCCACAGCG CCCCACCGCC CGCCTGCAGC 
ACCGACTTCC TGCACCTGGG AGATCTACCA GCAGAGCCTG GCAAGATGAC GGCCTCCACC 
AGCCACCCGG ATCTGCTGGG GGGGTGGGAG GCCTGGGCCG AGGCGCCGGC CCCCGCAGCT 
GCTGCAGAAG GAGGCCAGCC TGCCGCCCCC AGTGCCCCCG GGAGCTGGAC CAAGTCTCAG 
AGCCTGGACC CGTTTGCTGA CCTCGGTGAC CTCAGTTCTG GCCTCCAAGG TGATGGAGCA 
GCACGTGAGG TTGGCGCAGC GCTGGAGTTG CTGGATGCGA TCACGGCTGG CGGGTCCCCA 
TGGCGGTCCC GGCCCTTCTC CCAGGCACTC TGGGCCTTCG TCGAGACTGC ATGGACTGGG 
AATCAGGAAC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022273000.1
CDS	16..3450
Translation	MRVSPKAGVKSAGLGAIRYAEAGAADARMLSAAPGRCSRRRRRGLCSDNTEHLCRVFNTRFTVDRGFAFVYEAQDLASGREYALKRLLSNEEEKNRAIIQEVCFMKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLTELCKGQLVDFLKKIESRGPLSCDTVLKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKFSIPPNDTRYSVFHDLIRATLKVNPEERLSITELVNQLQEIAAARNVNPKSPITELLEQNGGYGNAVPSRGPPPPGGSSSGYSGGLALAEYDQPYGGLLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRLFLDSKHPGHYAVYNLSPRTYRPSKFHNRVSECGWAARRAPNLRSLYSVCRNMHSWLRQDHKNVCVVHCMDGRAASAVAVCSFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYMCDMVAEEPITPHSKPILVKAVVMTPVPLFSKQRNGCRPFCEVYVGDERVTTTSQEYDKMRDFRIEDGKAVIPLGITVQGDVLIVIYHARSTLGGRLQAKVASMKMFQVQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNVEVEVEPRDRPSREAPPWESTSMRGLNPKILFSSREEQQDILSKFGKPELPRQPGSTAQYDAEAGSLDAEPTESDSPQSSGTDTSHFLHTLDWHEEKDTETSPESHFAKENLSTLAEDAGGSEPSDEEAATPSTESRDAADEDTPGPAARLPERDLIFDGATPATPQEPTRQEDSVDLLGLHAEAGLAPPVQASGAAPSTADLLSRLLGGPDAAPEVPPGDLLGGEAPLLFASPAPSSSAQSPPREGPAADPFDPLLLPSGPNTQPCSQPDLFGEFLHSESPAAVPASFPSTHSAPPPACSTDFLHLGDLPAEPGKMTASTSHPDLLGGWEAWAEAPAPAAAAEGGQPAAPSAPGSWTKSQSLDPFADLGDLSSGLQGDGAAREVGAALELLDAITAGGSPWRSRPFSQALWAFVETAWTGNQEQ

Target ORF information:

RefSeq Version	XM_022417292.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris cyclin G associated kinase (GAK), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022417292.1

ORF Insert Sequence:

ATGCGTGTTT CCCCCAAGGC CGGGGTGAAG AGCGCAGGTT TGGGTGCGAT CCGGTACGCA 
GAGGCGGGGG CCGCTGACGC CCGGATGCTG TCGGCCGCCC CGGGGCGCTG CAGCCGTCGC 
CGCCGTCGGG GCCTGTGCTC CGACAACACC GAACACTTGT GCCGGGTTTT TAATACGAGA 
TTCACGGTGG ACAGAGGGTT TGCATTTGTG TATGAAGCTC AAGATCTGGC AAGTGGCAGA 
GAATATGCCT TAAAGAGATT ATTGTCTAAC GAAGAGGAAA AGAACAGAGC CATCATTCAG 
GAAGTTTGTT TCATGAAAAA ACTTTCTGGC CACCCCAATA TTGTCCAGTT TTGTTCTGCA 
GCGTCCATAG GGAAAGAGGA GTCGGACACT GGGCAGGCTG AGTTCCTGCT GCTCACCGAG 
CTCTGCAAAG GGCAGCTGGT GGACTTTTTA AAGAAAATTG AATCTAGAGG TCCGCTCTCG 
TGCGATACTG TTCTGAAGAT TTTCTATCAG ACGTGCAGAG CGGTGCAGCA CATGCACAGA 
CAAAAGCCGC CCATCATCCA TAGAGACCTC AAGGTGGAAA ACTTATTGCT CAGTAACCAA 
GGGACCATCA AGCTGTGTGA CTTTGGCAGT GCCACGACCA TATCACATTA CCCCGACTAC 
AGCTGGAGCG CCCAGAGGCG GGCCCTGGTG GAGGAAGAGA TCACCAGGAA CACCACACCC 
ATGTACAGGA CACCGGAAAT CGTGGACTTG TACTCCAACT TCCCCATCGG CGAGAAGCAG 
GACATCTGGG CGCTGGGCTG CATCTTGTAT CTGCTGTGCT TCCGGCAGCA TCCTTTTGAA 
GACGGAGCAA AGCTTCGGAT AGTCAACGGG AAGTTCTCGA TCCCCCCCAA TGACACCCGG 
TACTCTGTGT TCCACGACCT CATCCGCGCC ACGCTGAAGG TCAACCCCGA GGAGCGGCTG 
TCCATCACAG AACTGGTGAA CCAGCTCCAG GAGATCGCGG CCGCCAGGAA CGTGAACCCC 
AAGTCTCCCA TCACTGAGCT CCTAGAGCAG AACGGAGGCT ATGGGAACGC TGTCCCTTCC 
CGAGGGCCAC CCCCTCCTGG GGGCTCCAGC AGCGGCTACA GCGGAGGCCT GGCGCTGGCC 
GAGTACGACC AGCCCTACGG CGGGCTCCTG GACATCCTGC GGGGGGGCAC GGAGCGCCTC 
TTCACAAACC TCAAGGACAC CTCCTCCAAG GTCATCCAGT CTGTTGCCAA TTACGCAAAA 
GGAGATTTGG ACATCTCTTA CATCACATCT AGAATTGCTG TGATGTCGTT CCCAGCAGAA 
GGCGTGGAGT CGGCCATCAA GAACAACATC GAAGACGTGC GGTTGTTTCT GGACTCCAAG 
CACCCGGGAC ATTACGCCGT CTACAACCTG TCCCCAAGAA CATACCGGCC CTCCAAATTT 
CACAACCGGG TCTCCGAGTG TGGCTGGGCG GCCAGACGGG CCCCGAACCT TCGCAGCCTG 
TACAGTGTGT GTCGGAACAT GCACTCGTGG CTCCGGCAGG ACCACAAGAA CGTCTGTGTC 
GTGCACTGCA TGGACGGGAG AGCTGCGTCT GCGGTGGCCG TGTGCTCCTT CCTGTGCTTC 
TGCCGCCTGT TCAGCACCGC GGAGGCTGCC GTGTACATGT TCAGCATGAA GCGCTGCCCG 
CCAGGCATTT GGCCATCCCA CAAGAGGTAC ATCGAGTACA TGTGTGACAT GGTGGCCGAG 
GAGCCCATCA CACCCCATAG CAAGCCCATC CTGGTGAAAG CCGTGGTCAT GACACCCGTG 
CCCCTGTTCA GCAAGCAGAG AAACGGCTGC AGGCCCTTCT GCGAGGTCTA CGTGGGGGAT 
GAGCGCGTGA CTACCACGTC TCAGGAGTAC GACAAGATGA GGGATTTCAG AATCGAGGAT 
GGCAAAGCAG TCATTCCCCT GGGCATAACA GTGCAAGGGG ACGTGCTCAT TGTCATCTAC 
CACGCCAGGT CCACCCTGGG AGGAAGACTG CAGGCCAAGG TGGCATCCAT GAAGATGTTC 
CAGGTCCAGT TCCACACGGG GTTTGTGCCT CGGAATGCGA CTACTGTGAA GTTTGCAAAG 
TATGACCTTG ATGCGTGTGA CATTCAGGAG AAATACCCAG ATTTATTCCA AGTGAACGTG 
GAGGTGGAGG TGGAGCCCAG AGACAGGCCC AGCCGGGAGG CCCCACCCTG GGAGAGCACC 
AGCATGAGGG GGCTCAACCC CAAAATCCTG TTTTCCAGCC GAGAGGAGCA GCAAGACATT 
CTGTCTAAAT TTGGGAAGCC GGAGCTCCCC AGGCAGCCCG GCTCCACCGC TCAGTACGAC 
GCCGAGGCAG GGTCCCTGGA CGCAGAGCCC ACAGAGTCTG ATTCACCACA GAGCAGCGGC 
ACTGACACCA GCCACTTCCT CCACACGCTG GATTGGCACG AGGAGAAAGA TACAGAGACG 
AGTCCAGAAA GTCACTTTGC CAAGGAGAAC CTATCAACCC TGGCCGAGGA TGCAGGTGGG 
AGTGAGCCTT CGGACGAGGA AGCAGCCACA CCATCCACCG AGAGCAGGGA TGCTGCGGAT 
GAGGACACAC CAGGGCCAGC GGCGAGGCTG CCAGAGCGAG ATTTGATTTT TGATGGGGCC 
ACGCCGGCCA CACCGCAGGA GCCCACACGG CAGGAGGACA GTGTCGACCT CCTAGGGCTG 
CATGCCGAGG CTGGGCTGGC CCCCCCTGTG CAGGCCAGTG GGGCAGCCCC CAGCACCGCC 
GACCTGCTCA GCCGCCTCCT GGGGGGCCCT GACGCTGCTC CGGAGGTGCC CCCGGGTGAC 
CTGCTGGGTG GAGAGGCTCC CCTCCTCTTC GCAAGCCCAG CCCCCTCCTC GAGCGCACAG 
AGCCCGCCCC GTGAGGGGCC AGCTGCCGAC CCCTTTGACC CTCTCCTGCT GCCATCTGGG 
CCCAACACCC AGCCGTGCTC CCAGCCTGAC CTGTTCGGTG AATTTCTTCA TTCGGAATCT 
CCTGCCGCCG TGCCTGCGTC CTTCCCGTCC ACCCACAGCG CCCCACCGCC CGCCTGCAGC 
ACCGACTTCC TGCACCTGGG AGATCTACCA GCAGAGCCTG GCAAGATGAC GGCCTCCACC 
AGCCACCCGG ATCTGCTGGG GGGGTGGGAG GCCTGGGCCG AGGCGCCGGC CCCCGCAGCT 
GCTGCAGAAG GAGGCCAGCC TGCCGCCCCC AGTGCCCCCG GGAGCTGGAC CAAGTCTCAG 
AGCCTGGACC CGTTTGCTGA CCTCGGTGAC CTCAGTTCTG GCCTCCAAGG TGATGGAGCA 
GCACGTGAGG TTGGCGCAGC GCTGGAGTTG CTGGATGCGA TCACGGCTGG CGGGTCCCCA 
TGGCGGTCCC GGCCCTTCTC CCAGGCACTC TGGGCCTTCG TCGAGACTGC ATGGACTGGG 
AATCAGGAAC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	GAK
Entrez Gene ID	479133
Full Name	cyclin G associated kinase
General protein information	Preferred Names cyclin G associated kinase Names cyclin-G-associated kinase
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Rattus norvegicus (Norway rat)	Gak	NP_112292.1
Homo sapiens (human)	GAK	NP_005246.2
Bos taurus (cattle)	GAK	NP_001039549.1
Danio rerio (zebrafish)	gak	XP_003198642.2
Pan troglodytes (chimpanzee)	GAK	XP_001140835.1
Mus musculus (house mouse)	Gak	NP_705797.1
Gallus gallus (chicken)	GAK	XP_424873.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003715	XP_310244.7
	GAK	XP_001094120.2
Canis lupus familiaris (dog)	GAK	XP_536279.4
Drosophila melanogaster (fruit fly)	aux	NP_001262243.1
Danio rerio (zebrafish)	LOC100151158	XP_001919224.1
Xenopus tropicalis (tropical clawed frog)	gak	NP_001016350.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

GAK cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

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