LOC478773 gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_535939.5	XP_535939.2	sodium channel protein type 2 subunit alpha
XM_014110824.2	XP_013966299.1	sodium channel protein type 2 subunit alpha isoform X1
XM_022414740.1	XP_022270448.1	sodium channel protein type 2 subunit alpha isoform X1
XM_014110825.2	XP_013966300.1	sodium channel protein type 2 subunit alpha isoform X1
XM_022414742.1	XP_022270450.1	sodium channel protein type 2 subunit alpha isoform X3
XM_014110824.1	XP_013966299.1	sodium channel protein type 2 subunit alpha
XM_014110825.1	XP_013966300.1	sodium channel protein type 2 subunit alpha
XM_535939.6	XP_535939.2	sodium channel protein type 2 subunit alpha isoform X1
XM_022414741.1	XP_022270449.1	sodium channel protein type 2 subunit alpha isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe284700	XM_022414742.1 Latest version!	Canis lupus familiaris sodium channel protein type 2 subunit alpha (LOC478773), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$713.30 $1019.00
OCe31043	XM_022414741.1 Latest version!	Canis lupus familiaris sodium channel protein type 2 subunit alpha (LOC478773), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe31042	XM_535939.6 Latest version!	Canis lupus familiaris sodium channel protein type 2 subunit alpha (LOC478773), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe31042	XM_014110824.2 Latest version!	Canis lupus familiaris sodium channel protein type 2 subunit alpha (LOC478773), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe31042	XM_014110825.2 Latest version!	Canis lupus familiaris sodium channel protein type 2 subunit alpha (LOC478773), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe31042	XM_022414740.1 Latest version!	Canis lupus familiaris sodium channel protein type 2 subunit alpha (LOC478773), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
View Old Accession Versions >>
OCe31042	XM_014110824.1	Canis lupus familiaris sodium channel protein type 2 subunit alpha (LOC478773), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe31042	XM_014110825.1	Canis lupus familiaris sodium channel protein type 2 subunit alpha (LOC478773), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe31042	XM_535939.5	Canis lupus familiaris sodium channel protein type 2 subunit alpha (LOC478773), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
Hide Old Accession Versions >>

LOC478773 ( XM_022414742.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022270450.1 Canis lupus familiaris sodium channel protein type 2 subunit alpha (LOC478773), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe284700
Clone ID Related Accession (Same CDS sequence)	XM_022414742.1
Accession Version	XM_022414742.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	sodium channel protein type 2 subunit alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006618.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTTTGA TTTGGGACTG CTGTAAACCA TGGTTAAAGG TGAAACATCT CGTCAACCTG 
GTTGTAATGG ACCCGTTTGT TGACCTGGCC ATCACCATCT GCATTGTCCT AAATACACTG 
TTTATGGCCA TGGAGCACTA TCCTATGACA GAGCAGTTCA GCAGTGTGCT GTCAGTTGGG 
AACCTGGTCT TCACTGGGAT CTTCACAGCA GAAATGTTCC TCAAGATAAT AGCCATGGAT 
CCATATTATT ACTTTCAAGA GGGCTGGAAT ATTTTTGATG GTTTAATCGT AAGCCTTAGT 
TTAATGGAAC TTGGTTTGGC AAACGTTGAA GGATTGTCAG TTCTCCGATC ATTTCGACTG 
CTCCGAGTTT TCAAATTGGC AAAATCCTGG CCCACCCTGA ATATGCTGAT AAAGATCATC 
GGCAACTCAG TGGGGGCTCT GGGCAACCTC ACCTTGGTGT TGGCCATCAT CGTCTTCATT 
TTTGCCGTGG TTGGCATGCA GCTCTTTGGT AAGAGCTACA AAGAATGTGT CTGCAAGATC 
TCCAATGATT GTGAGCTCCC ACGGTGGCAC ATGCATGACT TCTTCCACTC CTTCCTGATT 
GTATTCCGTG TGTTGTGTGG AGAGTGGATA GAAACCATGT GGGACTGTAT GGAGGTCGCT 
GGCCAAACCA TGTGCCTTAC TGTCTTCATG ATGGTCATGG TGATTGGAAA CCTGGTGGTT 
CTGAATCTCT TTCTGGCCTT GCTCTTGAGT TCATTCAGTT CTGACAACCT TGCAGCCACT 
GACGATGATA ATGAAATGAA CAATCTCCAG ATTGCTGTGG GAAGGATGCA GAAAGGAATC 
GATTATGTTA AAAGAAAACT ACGTGAATTT ATTCAGAAAG CCTTTGTTAG AAAGCAGAAA 
GCCTTAGATG AAATTAAACC TCTTGAAGAT CTAAATAACA AAAAAGACAG CTGTATTTCC 
AACCATACAA CTATAGAAAT AGGAAAAGAT CTCAATTATC TCAAAGATGG AAATGGAACT 
ACCAGTGGCA TAGGCAGCAG TGTGGAAAAA TATGTTGTGG ATGAAAGTGA TTACATGTCA 
TTTATAAACA ATCCCAGCCT CACTGTGACT GTACCAATTG CTGTCGGGGA ATCTGACTTT 
GAAAATTTAA ATACTGAAGA ATTTAGCAGC GAGTCAGATA TGGAGGAAAG CAAAGAGAAG 
CTAAATGCAA CTAGTTCATC TGAAGGCAGC ACAGTTGATA TAGGAGCTCC CGCTGAGGGA 
GAGCAACCTG AGGTAGAACC CGAGGAATCT CTTGAACCTG AAGCCTGTTT CACGGAAGAC 
TGTGTACGGA AGTTCAAGTG TTGTCAGATA AGCATAGAAG AAGGCAAAGG GAAGCTCTGG 
TGGAACTTGA GAAAAACTTG CTATAAGATA GTGGAGCACA ACTGGTTTGA AACTTTCATT 
GTCTTCATGA TTCTTCTCAG CAGTGGGGCA CTGGCCTTTG AAGATATATA TATTGAGCAG 
CGAAAAACCA TTAAGACCAT GTTAGAATAT GCCGACAAAG TTTTCACTTA CATATTCATT 
CTGGAAATGC TTCTCAAGTG GGTCGCATAT GGTTTTCAAA TGTATTTCAC CAATGCCTGG 
TGCTGGCTGG ATTTCCTGAT TGTTGACGTC TCATTGGTTA GCTTGACTGC AAACGCCTTG 
GGTTACTCAG AACTTGGCGC CATCAAATCC CTCAGGACAC TCAGAGCTCT GAGGCCATTG 
AGAGCCCTAT CCCGGTTTGA AGGAATGAGG GTTGTTGTCA ATGCTCTTCT AGGAGCCATT 
CCATCCATAA TGAATGTGCT CCTGGTTTGT CTTATCTTTT GGCTAATATT CAGCATCATG 
GGAGTAAATC TTTTCGCTGG CAAGTTTTAC CATTGTATTA ACTACACCAC TGGAGAAATG 
TTTGATGTAA GTGTGGTCAA CAACTACAGC GAATGTAAAG CCCTCATAGA GAGCAATCAA 
ACTGCCAGGT GGAAAAATGT GAAAGTAAAC TTTGATAATG TAGGACTTGG ATATCTTTCT 
CTGCTTCAAG TAGCCACATT TAAGGGATGG ATGGACATCA TGTATGCAGC TGTTGATTCA 
CGAAATGTAG AATTACAACC CAAGTATGAA GACAATCTGT ACATGTACCT TTATTTTGTC 
ATCTTTATTA TTTTTGGTTC ATTCTTTACC CTGAATCTTT TCATTGGTGT CATCATAGAT 
AACTTCAACC AACAGAAAAA GAAGTTTGGA GGTCAAGACA TTTTTATGAC AGAAGAGCAG 
AAGAAATACT ACAATGCAAT GAAAAAACTG GGTTCAAAGA AACCACAAAA ACCCATACCT 
CGACCTGCTA ATAAATTCCA AGGAATGGTC TTTGATTTTG TAACCAAACA AGTCTTTGAT 
ATCAGTATCA TGATCCTCAT CTGCCTCAAC ATGGTCACCA TGATGGTGGA AACTGATGAC 
CAGAGTCAGG AGATGACAAA CATTCTGTAC TGGATTAATC TGGTGTTTAT TGTTCTGTTC 
ACTGGAGAAT GTGTGCTGAA ATTAATCTCC CTTCGTTATT ACTATTTCAC CATTGGATGG 
AATATTTTTG ATTTTGTGGT GGTCATTCTC TCTATCGTAG GCATGTTTCT GGCTGAGCTG 
ATAGAAAAAT ACTTTGTGTC CCCCACCCTG TTCCGAGTGA TCCGGCTCGC CAGGATCGGT 
CGAATCTTGC GTCTGATCAA AGGGGCGAAG GGGATCCGCA CACTGCTCTT TGCTCTGATG 
ATGTCCCTCC CTGCCTTGTT TAACATCGGC CTCCTGCTCT TCCTGGTCAT GTTCATCTAC 
GCCATCTTTG GAATGTCCAA CTTTGCCTAT GTCAAGAGGG AAGTTGGAAT TGATGACATG 
TTCAACTTTG AGACCTTTGG CAACAGCATG ATCTGCTTGT TCCAAATTAC CACCTCGGCT 
GGCTGGGATG GATTGCTAGC ACCTATTCTT AATAGTGGAC CTCCAGACTG TGATCCTGAA 
AAAGATCACC CTGGAAGCTC AGTTAAGGGA GACTGTGGGA ACCCATCTGT GGGGATTTTC 
TTTTTTGTCA GTTACATCAT CATCTCCTTT CTGGTTGTGG TGAACATGTA CATCGCTGTC 
ATCCTGGAGA ACTTCAGTGT TGCCACTGAG GAGAGTGCAG AGCCCCTGAG CGAGGATGAC 
TTTGAGATGT TCTATGAAGT CTGGGAGAAG TTTGATCCTG ATGCCACCCA GTTTATAGAG 
TTCTCCAAGC TCTCTGATTT TGCAGCTGCC CTGGATCCTC CTCTTCTCAT AGCGAAACCA 
AACAAAGTCC AGCTCATTGC CATGGACCTG CCCATGGTCA GTGGGGACCG GATCCACTGC 
CTCGACATTT TATTTGCCTT TACAAAGCGT GTTTTGGGTG AGAGTGGAGA GATGGATGCC 
CTTAGAATAC AGATGGAGGA GCGATTCATG GCATCGAACC CCTCCAAAGT CTCCTATGAA 
CCCATCACAA CCACTCTGAA ACGCAAACAA GAGGAGGTGT CTGCTATCGT TATCCAGAGG 
GCTTATAGAC GCTACCTCTT GAAGCAGAAA GTTAAAAAGG TTTCATGTAT CTATAAGAAA 
GACAAAGGCA AAGAAGGGGA TGGAACACCT ATCAAAGAAG ATATCCTCAT TGACAAACTG 
AATGAGAATT CGACGCCAGA GAAAACCGAT GTGACACCTT CCACCACTTC TCCACCTTCG 
TATGATAGTG TGACCAAACC AGAAAAAGAA AAATTTGAAA AAGACAAATC AGAAAAGGAA 
GACAAAGGGA AAGATATCAG GGAAAGTAAA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022270450.1
CDS	208..4023
Translation	MCLIWDCCKPWLKVKHLVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTEQFSSVLSVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGLIVSLSLMELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKISNDCELPRWHMHDFFHSFLIVFRVLCGEWIETMWDCMEVAGQTMCLTVFMMVMVIGNLVVLNLFLALLLSSFSSDNLAATDDDNEMNNLQIAVGRMQKGIDYVKRKLREFIQKAFVRKQKALDEIKPLEDLNNKKDSCISNHTTIEIGKDLNYLKDGNGTTSGIGSSVEKYVVDESDYMSFINNPSLTVTVPIAVGESDFENLNTEEFSSESDMEESKEKLNATSSSEGSTVDIGAPAEGEQPEVEPEESLEPEACFTEDCVRKFKCCQISIEEGKGKLWWNLRKTCYKIVEHNWFETFIVFMILLSSGALAFEDIYIEQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGFQMYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINYTTGEMFDVSVVNNYSECKALIESNQTARWKNVKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEDNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPANKFQGMVFDFVTKQVFDISIMILICLNMVTMMVETDDQSQEMTNILYWINLVFIVLFTGECVLKLISLRYYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSGPPDCDPEKDHPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFIEFSKLSDFAAALDPPLLIAKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYEPITTTLKRKQEEVSAIVIQRAYRRYLLKQKVKKVSCIYKKDKGKEGDGTPIKEDILIDKLNENSTPEKTDVTPSTTSPPSYDSVTKPEKEKFEKDKSEKEDKGKDIRESKK

Target ORF information:

RefSeq Version	XM_022414742.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris sodium channel protein type 2 subunit alpha (LOC478773), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022414742.1

ORF Insert Sequence:

ATGTGTTTGA TTTGGGACTG CTGTAAACCA TGGTTAAAGG TGAAACATCT CGTCAACCTG 
GTTGTAATGG ACCCGTTTGT TGACCTGGCC ATCACCATCT GCATTGTCCT AAATACACTG 
TTTATGGCCA TGGAGCACTA TCCTATGACA GAGCAGTTCA GCAGTGTGCT GTCAGTTGGG 
AACCTGGTCT TCACTGGGAT CTTCACAGCA GAAATGTTCC TCAAGATAAT AGCCATGGAT 
CCATATTATT ACTTTCAAGA GGGCTGGAAT ATTTTTGATG GTTTAATCGT AAGCCTTAGT 
TTAATGGAAC TTGGTTTGGC AAACGTTGAA GGATTGTCAG TTCTCCGATC ATTTCGACTG 
CTCCGAGTTT TCAAATTGGC AAAATCCTGG CCCACCCTGA ATATGCTGAT AAAGATCATC 
GGCAACTCAG TGGGGGCTCT GGGCAACCTC ACCTTGGTGT TGGCCATCAT CGTCTTCATT 
TTTGCCGTGG TTGGCATGCA GCTCTTTGGT AAGAGCTACA AAGAATGTGT CTGCAAGATC 
TCCAATGATT GTGAGCTCCC ACGGTGGCAC ATGCATGACT TCTTCCACTC CTTCCTGATT 
GTATTCCGTG TGTTGTGTGG AGAGTGGATA GAAACCATGT GGGACTGTAT GGAGGTCGCT 
GGCCAAACCA TGTGCCTTAC TGTCTTCATG ATGGTCATGG TGATTGGAAA CCTGGTGGTT 
CTGAATCTCT TTCTGGCCTT GCTCTTGAGT TCATTCAGTT CTGACAACCT TGCAGCCACT 
GACGATGATA ATGAAATGAA CAATCTCCAG ATTGCTGTGG GAAGGATGCA GAAAGGAATC 
GATTATGTTA AAAGAAAACT ACGTGAATTT ATTCAGAAAG CCTTTGTTAG AAAGCAGAAA 
GCCTTAGATG AAATTAAACC TCTTGAAGAT CTAAATAACA AAAAAGACAG CTGTATTTCC 
AACCATACAA CTATAGAAAT AGGAAAAGAT CTCAATTATC TCAAAGATGG AAATGGAACT 
ACCAGTGGCA TAGGCAGCAG TGTGGAAAAA TATGTTGTGG ATGAAAGTGA TTACATGTCA 
TTTATAAACA ATCCCAGCCT CACTGTGACT GTACCAATTG CTGTCGGGGA ATCTGACTTT 
GAAAATTTAA ATACTGAAGA ATTTAGCAGC GAGTCAGATA TGGAGGAAAG CAAAGAGAAG 
CTAAATGCAA CTAGTTCATC TGAAGGCAGC ACAGTTGATA TAGGAGCTCC CGCTGAGGGA 
GAGCAACCTG AGGTAGAACC CGAGGAATCT CTTGAACCTG AAGCCTGTTT CACGGAAGAC 
TGTGTACGGA AGTTCAAGTG TTGTCAGATA AGCATAGAAG AAGGCAAAGG GAAGCTCTGG 
TGGAACTTGA GAAAAACTTG CTATAAGATA GTGGAGCACA ACTGGTTTGA AACTTTCATT 
GTCTTCATGA TTCTTCTCAG CAGTGGGGCA CTGGCCTTTG AAGATATATA TATTGAGCAG 
CGAAAAACCA TTAAGACCAT GTTAGAATAT GCCGACAAAG TTTTCACTTA CATATTCATT 
CTGGAAATGC TTCTCAAGTG GGTCGCATAT GGTTTTCAAA TGTATTTCAC CAATGCCTGG 
TGCTGGCTGG ATTTCCTGAT TGTTGACGTC TCATTGGTTA GCTTGACTGC AAACGCCTTG 
GGTTACTCAG AACTTGGCGC CATCAAATCC CTCAGGACAC TCAGAGCTCT GAGGCCATTG 
AGAGCCCTAT CCCGGTTTGA AGGAATGAGG GTTGTTGTCA ATGCTCTTCT AGGAGCCATT 
CCATCCATAA TGAATGTGCT CCTGGTTTGT CTTATCTTTT GGCTAATATT CAGCATCATG 
GGAGTAAATC TTTTCGCTGG CAAGTTTTAC CATTGTATTA ACTACACCAC TGGAGAAATG 
TTTGATGTAA GTGTGGTCAA CAACTACAGC GAATGTAAAG CCCTCATAGA GAGCAATCAA 
ACTGCCAGGT GGAAAAATGT GAAAGTAAAC TTTGATAATG TAGGACTTGG ATATCTTTCT 
CTGCTTCAAG TAGCCACATT TAAGGGATGG ATGGACATCA TGTATGCAGC TGTTGATTCA 
CGAAATGTAG AATTACAACC CAAGTATGAA GACAATCTGT ACATGTACCT TTATTTTGTC 
ATCTTTATTA TTTTTGGTTC ATTCTTTACC CTGAATCTTT TCATTGGTGT CATCATAGAT 
AACTTCAACC AACAGAAAAA GAAGTTTGGA GGTCAAGACA TTTTTATGAC AGAAGAGCAG 
AAGAAATACT ACAATGCAAT GAAAAAACTG GGTTCAAAGA AACCACAAAA ACCCATACCT 
CGACCTGCTA ATAAATTCCA AGGAATGGTC TTTGATTTTG TAACCAAACA AGTCTTTGAT 
ATCAGTATCA TGATCCTCAT CTGCCTCAAC ATGGTCACCA TGATGGTGGA AACTGATGAC 
CAGAGTCAGG AGATGACAAA CATTCTGTAC TGGATTAATC TGGTGTTTAT TGTTCTGTTC 
ACTGGAGAAT GTGTGCTGAA ATTAATCTCC CTTCGTTATT ACTATTTCAC CATTGGATGG 
AATATTTTTG ATTTTGTGGT GGTCATTCTC TCTATCGTAG GCATGTTTCT GGCTGAGCTG 
ATAGAAAAAT ACTTTGTGTC CCCCACCCTG TTCCGAGTGA TCCGGCTCGC CAGGATCGGT 
CGAATCTTGC GTCTGATCAA AGGGGCGAAG GGGATCCGCA CACTGCTCTT TGCTCTGATG 
ATGTCCCTCC CTGCCTTGTT TAACATCGGC CTCCTGCTCT TCCTGGTCAT GTTCATCTAC 
GCCATCTTTG GAATGTCCAA CTTTGCCTAT GTCAAGAGGG AAGTTGGAAT TGATGACATG 
TTCAACTTTG AGACCTTTGG CAACAGCATG ATCTGCTTGT TCCAAATTAC CACCTCGGCT 
GGCTGGGATG GATTGCTAGC ACCTATTCTT AATAGTGGAC CTCCAGACTG TGATCCTGAA 
AAAGATCACC CTGGAAGCTC AGTTAAGGGA GACTGTGGGA ACCCATCTGT GGGGATTTTC 
TTTTTTGTCA GTTACATCAT CATCTCCTTT CTGGTTGTGG TGAACATGTA CATCGCTGTC 
ATCCTGGAGA ACTTCAGTGT TGCCACTGAG GAGAGTGCAG AGCCCCTGAG CGAGGATGAC 
TTTGAGATGT TCTATGAAGT CTGGGAGAAG TTTGATCCTG ATGCCACCCA GTTTATAGAG 
TTCTCCAAGC TCTCTGATTT TGCAGCTGCC CTGGATCCTC CTCTTCTCAT AGCGAAACCA 
AACAAAGTCC AGCTCATTGC CATGGACCTG CCCATGGTCA GTGGGGACCG GATCCACTGC 
CTCGACATTT TATTTGCCTT TACAAAGCGT GTTTTGGGTG AGAGTGGAGA GATGGATGCC 
CTTAGAATAC AGATGGAGGA GCGATTCATG GCATCGAACC CCTCCAAAGT CTCCTATGAA 
CCCATCACAA CCACTCTGAA ACGCAAACAA GAGGAGGTGT CTGCTATCGT TATCCAGAGG 
GCTTATAGAC GCTACCTCTT GAAGCAGAAA GTTAAAAAGG TTTCATGTAT CTATAAGAAA 
GACAAAGGCA AAGAAGGGGA TGGAACACCT ATCAAAGAAG ATATCCTCAT TGACAAACTG 
AATGAGAATT CGACGCCAGA GAAAACCGAT GTGACACCTT CCACCACTTC TCCACCTTCG 
TATGATAGTG TGACCAAACC AGAAAAAGAA AAATTTGAAA AAGACAAATC AGAAAAGGAA 
GACAAAGGGA AAGATATCAG GGAAAGTAAA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC478773 ( XM_022414741.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022270449.1 Canis lupus familiaris sodium channel protein type 2 subunit alpha (LOC478773), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe31043
Clone ID Related Accession (Same CDS sequence)	XM_022414741.1
Accession Version	XM_022414741.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	sodium channel protein type 2 subunit alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006618.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGT CAGTGCTGGT ACCACCAGGA CCTGACAGCT TCCGCTTCTT TACCAGGGAA 
TCCCTTGCTG CTATTGAACA ACGCATTGCA GAAGAGAAAG CTAAGAGACC CAAACAGGAG 
CGTAAGGATG AGGATGATGA AAATGGCCCA AAGCCAAACA GTGACTTGGA AGCAGGGAAA 
TCTCTTCCAT TTATTTATGG AGACATTCCT CCAGAGATGG TGTCAGAGCC CTTGGAGGAC 
CTGGACCCGT ACTATATCAA TAAGAAAACA TTTATAGTAT TGAATAAAGG GAAAGCAATC 
TCTCGATTCA GTGCCACCCC TGCCCTGTAC ATTTTAACTC CCTTCAACCC TATTAGAAAA 
TTAGCTATTA AGATTTTGGT ACATTCTTTA TTCAATGTGC TCATTATGTG CACTATTCTC 
ACCAACTGTG TATTTATGAC CATGAGTAAC CCTCCAGACT GGACAAAGAA TGTGGAGTAC 
ACCTTTACAG GAATTTATAC ATTTGAATCA CTTATAAAAA TACTTGCAAG GGGCTTTTGT 
TTAGAAGATT TCACATTTCT CCGGGATCCA TGGAATTGGT TGGATTTCAC AGTCATTACT 
TTTGCGTATG TAACAGAATT TGTAAACCTA GGCAATGTTT CAGCTCTTCG AACTTTCAGA 
GTCTTGAGAG CTTTGAAAAC TATTTCTGTA ATTCCAGGCC TGAAGACCAT TGTGGGGGCC 
CTGATCCAGT CGGTGAAGAA GCTCTCTGAT GTAATGATTC TGACTGTGTT CTGTCTGAGT 
GTGTTTGCAC TCATAGGACT GCAGCTGTTT ATGGGCAACC TGCGGAATAA ATGCTTGCAA 
TGGCCCCCAG ATAATTCTTC CTTTGAAATA AACATCACTT CCTTCTTTAA TAATTCATTG 
GATGGGAACG GTACCATGTT CAATAGGACA GTGAGCATGT TTAACTGGGA TGAATATATT 
GAGGATAAAA GTCACTTTTA TTTTTTGGAA GGGCAAAATG ATGCTCTGCT TTGTGGCAAC 
AGCTCAGATG CAGGCCAGTG TCCGGAAGGA TATATCTGTG TGAAGGCTGG TAGAAACCCC 
AACTATGGCT ATACAAGCTT TGATACCTTT AGTTGGGCCT TCTTGTCATT ATTTCGTCTC 
ATGACTCAAG ACTTCTGGGA AAACCTTTAC CAACTGACGC TACGTGCTGC TGGGAAAACA 
TATATGATAT TTTTTGTGCT GGTCATTTTC TTGGGCTCAT TCTACCTTAT AAATTTAATC 
CTGGCTGTGG TGGCTATGGC CTACGAGGAA CAGAATCAGG CAACTCTTGA GGAAGCTGAA 
CAGAAAGAAG CTGAATTTCA GCAGATGCTC GAACAACTAA AAAAACAACA AGAAGAAGCT 
CAGGCAGCAG CTGCAGCCGC ATCTGCAGAA TCAAGAGAGT TCAGTGGTGC TGGTGGGATA 
GGAGTTTTCT CAGAGAGTTC TTCAGTAGCA TCAAAGTTGA GCTCCAAGAG TGAAAAAGAG 
CTGAAAAACA GAAGGAAGAA AAAGAAGCAG AAAGAGCAGT CTGGAGAAGA AGAGAAGGAA 
GATGGGGTTC GCAAATCCGA ATCCGAAGAC AGTATAAGAA GAAAAGGTTT CCGTTTTTCC 
TTAGAAGGAA GCAGGCTGAC ATATGAAAAA AGGTTCTCTT CTCCACATCA GTCTTTATTG 
AGCATCCGTG GCTCCCTTTT CTCTCCAAGA CGCAACAGTA GGGCGAGCCT TTTCAGTTTC 
AGAGGCCGAA CAAAGGATAT TGGCTCTGAG AACGACTTTG CTGATGATGA GCACAGCACC 
TTTGAAGACA ATGACAGCCG GAGAGACTCT CTGTTTGTGC CACACAGACA TGGAGAGCGG 
CGTCATAGCA ATGTCAGCCA GGCCAGCCGG GCCTCCAGGG TGATCCCCAT CCTGCCGGTG 
AATGGAAAGA TGCACAGTGC AGTGGATTGC AACGGGGTCG TCTCGCTGGT TGGGGGCCCT 
TCTGCCCTCA CGTCTCCAGC TGGACAGCTC CTTCCGGAGG GCACGACTAC TGAAACAGAA 
CTAAGAAAGA GACGGTCCAG TTCTTACCAT GTTTCCATGG ATTTGCTGGA AGATCCTACC 
ACAAGGCAAA GAGCAATGAG TCTAGCCAGT ATTTTGACCA ACACCATGGA AGAACTTGAA 
GAATCCAGAC AGAAATGCCC ACCCTGTTGG TATAAATTTG CTAACATGTG TTTGATTTGG 
GACTGCTGTA AACCATGGTT AAAGGTGAAA CATCTCGTCA ACCTGGTTGT AATGGACCCG 
TTTGTTGACC TGGCCATCAC CATCTGCATT GTCCTAAATA CACTGTTTAT GGCCATGGAG 
CACTATCCTA TGACAGAGCA GTTCAGCAGT GTGCTGTCAG TTGGGAACCT GGTCTTCACT 
GGGATCTTCA CAGCAGAAAT GTTCCTCAAG ATAATAGCCA TGGATCCATA TTATTACTTT 
CAAGAGGGCT GGAATATTTT TGATGGTTTA ATCGTAAGCC TTAGTTTAAT GGAACTTGGT 
TTGGCAAACG TTGAAGGATT GTCAGTTCTC CGATCATTTC GACTGCTCCG AGTTTTCAAA 
TTGGCAAAAT CCTGGCCCAC CCTGAATATG CTGATAAAGA TCATCGGCAA CTCAGTGGGG 
GCTCTGGGCA ACCTCACCTT GGTGTTGGCC ATCATCGTCT TCATTTTTGC CGTGGTTGGC 
ATGCAGCTCT TTGGTAAGAG CTACAAAGAA TGTGTCTGCA AGATCTCCAA TGATTGTGAG 
CTCCCACGGT GGCACATGCA TGACTTCTTC CACTCCTTCC TGATTGTATT CCGTGTGTTG 
TGTGGAGAGT GGATAGAAAC CATGTGGGAC TGTATGGAGG TCGCTGGCCA AACCATGTGC 
CTTACTGTCT TCATGATGGT CATGGTGATT GGAAACCTGG TGGTTCTGAA TCTCTTTCTG 
GCCTTGCTCT TGAGTTCATT CAGTTCTGAC AACCTTGCAG CCACTGACGA TGATAATGAA 
ATGAACAATC TCCAGATTGC TGTGGGAAGG ATGCAGAAAG GAATCGATTA TGTTAAAAGA 
AAACTACGTG AATTTATTCA GAAAGCCTTT GTTAGAAAGC AGAAAGCCTT AGATGAAATT 
AAACCTCTTG AAGATCTAAA TAACAAAAAA GACAGCTGTA TTTCCAACCA TACAACTATA 
GAAATAGGAA AAGATCTCAA TTATCTCAAA GATGGAAATG GAACTACCAG TGGCATAGGC 
AGCAGTGTGG AAAAATATGT TGTGGATGAA AGTGATTACA TGTCATTTAT AAACAATCCC 
AGCCTCACTG TGACTGTACC AATTGCTGTC GGGGAATCTG ACTTTGAAAA TTTAAATACT 
GAAGAATTTA GCAGCGAGTC AGATATGGAG GAAAGCAAAG AGAAGCTAAA TGCAACTAGT 
TCATCTGAAG GCAGCACAGT TGATATAGGA GCTCCCGCTG AGGGAGAGCA ACCTGAGGTA 
GAACCCGAGG AATCTCTTGA ACCTGAAGCC TGTTTCACGG AAGACTGTGT ACGGAAGTTC 
AAGTGTTGTC AGATAAGCAT AGAAGAAGGC AAAGGGAAGC TCTGGTGGAA CTTGAGAAAA 
ACTTGCTATA AGATAGTGGA GCACAACTGG TTTGAAACTT TCATTGTCTT CATGATTCTT 
CTCAGCAGTG GGGCACTGGC CTTTGAAGAT ATATATATTG AGCAGCGAAA AACCATTAAG 
ACCATGTTAG AATATGCCGA CAAAGTTTTC ACTTACATAT TCATTCTGGA AATGCTTCTC 
AAGTGGGTCG CATATGGTTT TCAAATGTAT TTCACCAATG CCTGGTGCTG GCTGGATTTC 
CTGATTGTTG ACGTCTCATT GGTTAGCTTG ACTGCAAACG CCTTGGGTTA CTCAGAACTT 
GGCGCCATCA AATCCCTCAG GACACTCAGA GCTCTGAGGC CATTGAGAGC CCTATCCCGG 
TTTGAAGGAA TGAGGGTTGT TGTCAATGCT CTTCTAGGAG CCATTCCATC CATAATGAAT 
GTGCTCCTGG TTTGTCTTAT CTTTTGGCTA ATATTCAGCA TCATGGGAGT AAATCTTTTC 
GCTGGCAAGT TTTACCATTG TATTAACTAC ACCACTGGAG AAATGTTTGA TGTAAGTGTG 
GTCAACAACT ACAGCGAATG TAAAGCCCTC ATAGAGAGCA ATCAAACTGC CAGGTGGAAA 
AATGTGAAAG TAAACTTTGA TAATGTAGGA CTTGGATATC TTTCTCTGCT TCAAGTAGCC 
ACATTTAAGG GATGGATGGA CATCATGTAT GCAGCTGTTG ATTCACGAAA TGTAGAATTA 
CAACCCAAGT ATGAAGACAA TCTGTACATG TACCTTTATT TTGTCATCTT TATTATTTTT 
GGTTCATTCT TTACCCTGAA TCTTTTCATT GGTGTCATCA TAGATAACTT CAACCAACAG 
AAAAAGAAGT TTGGAGGTCA AGACATTTTT ATGACAGAAG AGCAGAAGAA ATACTACAAT 
GCAATGAAAA AACTGGGTTC AAAGAAACCA CAAAAACCCA TACCTCGACC TGCTAATAAA 
TTCCAAGGAA TGGTCTTTGA TTTTGTAACC AAACAAGTCT TTGATATCAG TATCATGATC 
CTCATCTGCC TCAACATGGT CACCATGATG GTGGAAACTG ATGACCAGAG TCAGGAGATG 
ACAAACATTC TGTACTGGAT TAATCTGGTG TTTATTGTTC TGTTCACTGG AGAATGTGTG 
CTGAAATTAA TCTCCCTTCG TTATTACTAT TTCACCATTG GATGGAATAT TTTTGATTTT 
GTGGTGGTCA TTCTCTCTAT CGTAGGCATG TTTCTGGCTG AGCTGATAGA AAAATACTTT 
GTGTCCCCCA CCCTGTTCCG AGTGATCCGG CTCGCCAGGA TCGGTCGAAT CTTGCGTCTG 
ATCAAAGGGG CGAAGGGGAT CCGCACACTG CTCTTTGCTC TGATGATGTC CCTCCCTGCC 
TTGTTTAACA TCGGCCTCCT GCTCTTCCTG GTCATGTTCA TCTACGCCAT CTTTGGAATG 
TCCAACTTTG CCTATGTCAA GAGGGAAGTT GGAATTGATG ACATGTTCAA CTTTGAGACC 
TTTGGCAACA GCATGATCTG CTTGTTCCAA ATTACCACCT CGGCTGGCTG GGATGGATTG 
CTAGCACCTA TTCTTAATAG TGGACCTCCA GACTGTGATC CTGAAAAAGA TCACCCTGGA 
AGCTCAGTTA AGGGAGACTG TGGGAACCCA TCTGTGGGGA TTTTCTTTTT TGTCAGTTAC 
ATCATCATCT CCTTTCTGGT TGTGGTGAAC ATGTACATCG CTGTCATCCT GGAGAACTTC 
AGTGTTGCCA CTGAGGAGAG TGCAGAGCCC CTGAGCGAGG ATGACTTTGA GATGTTCTAT 
GAAGTCTGGG AGAAGTTTGA TCCTGATGCC ACCCAGTTTA TAGAGTTCTC CAAGCTCTCT 
GATTTTGCAG CTGCCCTGGA TCCTCCTCTT CTCATAGCGA AACCAAACAA AGTCCAGCTC 
ATTGCCATGG ACCTGCCCAT GGTCAGTGGG GACCGGATCC ACTGCCTCGA CATTTTATTT 
GCCTTTACAA AGCGTGTTTT GGGTGAGAGT GGAGAGATGG ATGCCCTTAG AATACAGATG 
GAGGAGCGAT TCATGGCATC GAACCCCTCC AAAGTCTCCT ATGAACCCAT CACAACCACT 
CTGAAACGCA AACAAGAGGA GGTGTCTGCT ATCGTTATCC AGAGGGCTTA TAGACGCTAC 
CTCTTGAAGC AGAAAGTTAA AAAGGTTTCA TGTATCTATA AGAAAGACAA AGGCAAAGAA 
GGGGATGGAA CACCTATCAA AGAAGATATC CTCATTGACA AACTGAATGA GAATTCGACG 
CCAGAGAAAA CCGATGTGAC ACCTTCCACC ACTTCTCCAC CTTCGTATGA TAGTGTGACC 
AAACCAGAAA AAGAAAAATT TGAAAAAGAC AAATCAGAAA AGGAAGACAA AGGGAAAGAT 
ATCAGGGAAA GTAAAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022270449.1
CDS	187..6207
Translation	MAQSVLVPPGPDSFRFFTRESLAAIEQRIAEEKAKRPKQERKDEDDENGPKPNSDLEAGKSLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAISRFSATPALYILTPFNPIRKLAIKILVHSLFNVLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKILARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVNLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCLQWPPDNSSFEINITSFFNNSLDGNGTMFNRTVSMFNWDEYIEDKSHFYFLEGQNDALLCGNSSDAGQCPEGYICVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMLEQLKKQQEEAQAAAAAASAESREFSGAGGIGVFSESSSVASKLSSKSEKELKNRRKKKKQKEQSGEEEKEDGVRKSESEDSIRRKGFRFSLEGSRLTYEKRFSSPHQSLLSIRGSLFSPRRNSRASLFSFRGRTKDIGSENDFADDEHSTFEDNDSRRDSLFVPHRHGERRHSNVSQASRASRVIPILPVNGKMHSAVDCNGVVSLVGGPSALTSPAGQLLPEGTTTETELRKRRSSSYHVSMDLLEDPTTRQRAMSLASILTNTMEELEESRQKCPPCWYKFANMCLIWDCCKPWLKVKHLVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTEQFSSVLSVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGLIVSLSLMELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKISNDCELPRWHMHDFFHSFLIVFRVLCGEWIETMWDCMEVAGQTMCLTVFMMVMVIGNLVVLNLFLALLLSSFSSDNLAATDDDNEMNNLQIAVGRMQKGIDYVKRKLREFIQKAFVRKQKALDEIKPLEDLNNKKDSCISNHTTIEIGKDLNYLKDGNGTTSGIGSSVEKYVVDESDYMSFINNPSLTVTVPIAVGESDFENLNTEEFSSESDMEESKEKLNATSSSEGSTVDIGAPAEGEQPEVEPEESLEPEACFTEDCVRKFKCCQISIEEGKGKLWWNLRKTCYKIVEHNWFETFIVFMILLSSGALAFEDIYIEQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGFQMYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINYTTGEMFDVSVVNNYSECKALIESNQTARWKNVKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEDNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPANKFQGMVFDFVTKQVFDISIMILICLNMVTMMVETDDQSQEMTNILYWINLVFIVLFTGECVLKLISLRYYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSGPPDCDPEKDHPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFIEFSKLSDFAAALDPPLLIAKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYEPITTTLKRKQEEVSAIVIQRAYRRYLLKQKVKKVSCIYKKDKGKEGDGTPIKEDILIDKLNENSTPEKTDVTPSTTSPPSYDSVTKPEKEKFEKDKSEKEDKGKDIRESKK

Target ORF information:

RefSeq Version	XM_022414741.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris sodium channel protein type 2 subunit alpha (LOC478773), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022414741.1

ORF Insert Sequence:

ATGGCACAGT CAGTGCTGGT ACCACCAGGA CCTGACAGCT TCCGCTTCTT TACCAGGGAA 
TCCCTTGCTG CTATTGAACA ACGCATTGCA GAAGAGAAAG CTAAGAGACC CAAACAGGAG 
CGTAAGGATG AGGATGATGA AAATGGCCCA AAGCCAAACA GTGACTTGGA AGCAGGGAAA 
TCTCTTCCAT TTATTTATGG AGACATTCCT CCAGAGATGG TGTCAGAGCC CTTGGAGGAC 
CTGGACCCGT ACTATATCAA TAAGAAAACA TTTATAGTAT TGAATAAAGG GAAAGCAATC 
TCTCGATTCA GTGCCACCCC TGCCCTGTAC ATTTTAACTC CCTTCAACCC TATTAGAAAA 
TTAGCTATTA AGATTTTGGT ACATTCTTTA TTCAATGTGC TCATTATGTG CACTATTCTC 
ACCAACTGTG TATTTATGAC CATGAGTAAC CCTCCAGACT GGACAAAGAA TGTGGAGTAC 
ACCTTTACAG GAATTTATAC ATTTGAATCA CTTATAAAAA TACTTGCAAG GGGCTTTTGT 
TTAGAAGATT TCACATTTCT CCGGGATCCA TGGAATTGGT TGGATTTCAC AGTCATTACT 
TTTGCGTATG TAACAGAATT TGTAAACCTA GGCAATGTTT CAGCTCTTCG AACTTTCAGA 
GTCTTGAGAG CTTTGAAAAC TATTTCTGTA ATTCCAGGCC TGAAGACCAT TGTGGGGGCC 
CTGATCCAGT CGGTGAAGAA GCTCTCTGAT GTAATGATTC TGACTGTGTT CTGTCTGAGT 
GTGTTTGCAC TCATAGGACT GCAGCTGTTT ATGGGCAACC TGCGGAATAA ATGCTTGCAA 
TGGCCCCCAG ATAATTCTTC CTTTGAAATA AACATCACTT CCTTCTTTAA TAATTCATTG 
GATGGGAACG GTACCATGTT CAATAGGACA GTGAGCATGT TTAACTGGGA TGAATATATT 
GAGGATAAAA GTCACTTTTA TTTTTTGGAA GGGCAAAATG ATGCTCTGCT TTGTGGCAAC 
AGCTCAGATG CAGGCCAGTG TCCGGAAGGA TATATCTGTG TGAAGGCTGG TAGAAACCCC 
AACTATGGCT ATACAAGCTT TGATACCTTT AGTTGGGCCT TCTTGTCATT ATTTCGTCTC 
ATGACTCAAG ACTTCTGGGA AAACCTTTAC CAACTGACGC TACGTGCTGC TGGGAAAACA 
TATATGATAT TTTTTGTGCT GGTCATTTTC TTGGGCTCAT TCTACCTTAT AAATTTAATC 
CTGGCTGTGG TGGCTATGGC CTACGAGGAA CAGAATCAGG CAACTCTTGA GGAAGCTGAA 
CAGAAAGAAG CTGAATTTCA GCAGATGCTC GAACAACTAA AAAAACAACA AGAAGAAGCT 
CAGGCAGCAG CTGCAGCCGC ATCTGCAGAA TCAAGAGAGT TCAGTGGTGC TGGTGGGATA 
GGAGTTTTCT CAGAGAGTTC TTCAGTAGCA TCAAAGTTGA GCTCCAAGAG TGAAAAAGAG 
CTGAAAAACA GAAGGAAGAA AAAGAAGCAG AAAGAGCAGT CTGGAGAAGA AGAGAAGGAA 
GATGGGGTTC GCAAATCCGA ATCCGAAGAC AGTATAAGAA GAAAAGGTTT CCGTTTTTCC 
TTAGAAGGAA GCAGGCTGAC ATATGAAAAA AGGTTCTCTT CTCCACATCA GTCTTTATTG 
AGCATCCGTG GCTCCCTTTT CTCTCCAAGA CGCAACAGTA GGGCGAGCCT TTTCAGTTTC 
AGAGGCCGAA CAAAGGATAT TGGCTCTGAG AACGACTTTG CTGATGATGA GCACAGCACC 
TTTGAAGACA ATGACAGCCG GAGAGACTCT CTGTTTGTGC CACACAGACA TGGAGAGCGG 
CGTCATAGCA ATGTCAGCCA GGCCAGCCGG GCCTCCAGGG TGATCCCCAT CCTGCCGGTG 
AATGGAAAGA TGCACAGTGC AGTGGATTGC AACGGGGTCG TCTCGCTGGT TGGGGGCCCT 
TCTGCCCTCA CGTCTCCAGC TGGACAGCTC CTTCCGGAGG GCACGACTAC TGAAACAGAA 
CTAAGAAAGA GACGGTCCAG TTCTTACCAT GTTTCCATGG ATTTGCTGGA AGATCCTACC 
ACAAGGCAAA GAGCAATGAG TCTAGCCAGT ATTTTGACCA ACACCATGGA AGAACTTGAA 
GAATCCAGAC AGAAATGCCC ACCCTGTTGG TATAAATTTG CTAACATGTG TTTGATTTGG 
GACTGCTGTA AACCATGGTT AAAGGTGAAA CATCTCGTCA ACCTGGTTGT AATGGACCCG 
TTTGTTGACC TGGCCATCAC CATCTGCATT GTCCTAAATA CACTGTTTAT GGCCATGGAG 
CACTATCCTA TGACAGAGCA GTTCAGCAGT GTGCTGTCAG TTGGGAACCT GGTCTTCACT 
GGGATCTTCA CAGCAGAAAT GTTCCTCAAG ATAATAGCCA TGGATCCATA TTATTACTTT 
CAAGAGGGCT GGAATATTTT TGATGGTTTA ATCGTAAGCC TTAGTTTAAT GGAACTTGGT 
TTGGCAAACG TTGAAGGATT GTCAGTTCTC CGATCATTTC GACTGCTCCG AGTTTTCAAA 
TTGGCAAAAT CCTGGCCCAC CCTGAATATG CTGATAAAGA TCATCGGCAA CTCAGTGGGG 
GCTCTGGGCA ACCTCACCTT GGTGTTGGCC ATCATCGTCT TCATTTTTGC CGTGGTTGGC 
ATGCAGCTCT TTGGTAAGAG CTACAAAGAA TGTGTCTGCA AGATCTCCAA TGATTGTGAG 
CTCCCACGGT GGCACATGCA TGACTTCTTC CACTCCTTCC TGATTGTATT CCGTGTGTTG 
TGTGGAGAGT GGATAGAAAC CATGTGGGAC TGTATGGAGG TCGCTGGCCA AACCATGTGC 
CTTACTGTCT TCATGATGGT CATGGTGATT GGAAACCTGG TGGTTCTGAA TCTCTTTCTG 
GCCTTGCTCT TGAGTTCATT CAGTTCTGAC AACCTTGCAG CCACTGACGA TGATAATGAA 
ATGAACAATC TCCAGATTGC TGTGGGAAGG ATGCAGAAAG GAATCGATTA TGTTAAAAGA 
AAACTACGTG AATTTATTCA GAAAGCCTTT GTTAGAAAGC AGAAAGCCTT AGATGAAATT 
AAACCTCTTG AAGATCTAAA TAACAAAAAA GACAGCTGTA TTTCCAACCA TACAACTATA 
GAAATAGGAA AAGATCTCAA TTATCTCAAA GATGGAAATG GAACTACCAG TGGCATAGGC 
AGCAGTGTGG AAAAATATGT TGTGGATGAA AGTGATTACA TGTCATTTAT AAACAATCCC 
AGCCTCACTG TGACTGTACC AATTGCTGTC GGGGAATCTG ACTTTGAAAA TTTAAATACT 
GAAGAATTTA GCAGCGAGTC AGATATGGAG GAAAGCAAAG AGAAGCTAAA TGCAACTAGT 
TCATCTGAAG GCAGCACAGT TGATATAGGA GCTCCCGCTG AGGGAGAGCA ACCTGAGGTA 
GAACCCGAGG AATCTCTTGA ACCTGAAGCC TGTTTCACGG AAGACTGTGT ACGGAAGTTC 
AAGTGTTGTC AGATAAGCAT AGAAGAAGGC AAAGGGAAGC TCTGGTGGAA CTTGAGAAAA 
ACTTGCTATA AGATAGTGGA GCACAACTGG TTTGAAACTT TCATTGTCTT CATGATTCTT 
CTCAGCAGTG GGGCACTGGC CTTTGAAGAT ATATATATTG AGCAGCGAAA AACCATTAAG 
ACCATGTTAG AATATGCCGA CAAAGTTTTC ACTTACATAT TCATTCTGGA AATGCTTCTC 
AAGTGGGTCG CATATGGTTT TCAAATGTAT TTCACCAATG CCTGGTGCTG GCTGGATTTC 
CTGATTGTTG ACGTCTCATT GGTTAGCTTG ACTGCAAACG CCTTGGGTTA CTCAGAACTT 
GGCGCCATCA AATCCCTCAG GACACTCAGA GCTCTGAGGC CATTGAGAGC CCTATCCCGG 
TTTGAAGGAA TGAGGGTTGT TGTCAATGCT CTTCTAGGAG CCATTCCATC CATAATGAAT 
GTGCTCCTGG TTTGTCTTAT CTTTTGGCTA ATATTCAGCA TCATGGGAGT AAATCTTTTC 
GCTGGCAAGT TTTACCATTG TATTAACTAC ACCACTGGAG AAATGTTTGA TGTAAGTGTG 
GTCAACAACT ACAGCGAATG TAAAGCCCTC ATAGAGAGCA ATCAAACTGC CAGGTGGAAA 
AATGTGAAAG TAAACTTTGA TAATGTAGGA CTTGGATATC TTTCTCTGCT TCAAGTAGCC 
ACATTTAAGG GATGGATGGA CATCATGTAT GCAGCTGTTG ATTCACGAAA TGTAGAATTA 
CAACCCAAGT ATGAAGACAA TCTGTACATG TACCTTTATT TTGTCATCTT TATTATTTTT 
GGTTCATTCT TTACCCTGAA TCTTTTCATT GGTGTCATCA TAGATAACTT CAACCAACAG 
AAAAAGAAGT TTGGAGGTCA AGACATTTTT ATGACAGAAG AGCAGAAGAA ATACTACAAT 
GCAATGAAAA AACTGGGTTC AAAGAAACCA CAAAAACCCA TACCTCGACC TGCTAATAAA 
TTCCAAGGAA TGGTCTTTGA TTTTGTAACC AAACAAGTCT TTGATATCAG TATCATGATC 
CTCATCTGCC TCAACATGGT CACCATGATG GTGGAAACTG ATGACCAGAG TCAGGAGATG 
ACAAACATTC TGTACTGGAT TAATCTGGTG TTTATTGTTC TGTTCACTGG AGAATGTGTG 
CTGAAATTAA TCTCCCTTCG TTATTACTAT TTCACCATTG GATGGAATAT TTTTGATTTT 
GTGGTGGTCA TTCTCTCTAT CGTAGGCATG TTTCTGGCTG AGCTGATAGA AAAATACTTT 
GTGTCCCCCA CCCTGTTCCG AGTGATCCGG CTCGCCAGGA TCGGTCGAAT CTTGCGTCTG 
ATCAAAGGGG CGAAGGGGAT CCGCACACTG CTCTTTGCTC TGATGATGTC CCTCCCTGCC 
TTGTTTAACA TCGGCCTCCT GCTCTTCCTG GTCATGTTCA TCTACGCCAT CTTTGGAATG 
TCCAACTTTG CCTATGTCAA GAGGGAAGTT GGAATTGATG ACATGTTCAA CTTTGAGACC 
TTTGGCAACA GCATGATCTG CTTGTTCCAA ATTACCACCT CGGCTGGCTG GGATGGATTG 
CTAGCACCTA TTCTTAATAG TGGACCTCCA GACTGTGATC CTGAAAAAGA TCACCCTGGA 
AGCTCAGTTA AGGGAGACTG TGGGAACCCA TCTGTGGGGA TTTTCTTTTT TGTCAGTTAC 
ATCATCATCT CCTTTCTGGT TGTGGTGAAC ATGTACATCG CTGTCATCCT GGAGAACTTC 
AGTGTTGCCA CTGAGGAGAG TGCAGAGCCC CTGAGCGAGG ATGACTTTGA GATGTTCTAT 
GAAGTCTGGG AGAAGTTTGA TCCTGATGCC ACCCAGTTTA TAGAGTTCTC CAAGCTCTCT 
GATTTTGCAG CTGCCCTGGA TCCTCCTCTT CTCATAGCGA AACCAAACAA AGTCCAGCTC 
ATTGCCATGG ACCTGCCCAT GGTCAGTGGG GACCGGATCC ACTGCCTCGA CATTTTATTT 
GCCTTTACAA AGCGTGTTTT GGGTGAGAGT GGAGAGATGG ATGCCCTTAG AATACAGATG 
GAGGAGCGAT TCATGGCATC GAACCCCTCC AAAGTCTCCT ATGAACCCAT CACAACCACT 
CTGAAACGCA AACAAGAGGA GGTGTCTGCT ATCGTTATCC AGAGGGCTTA TAGACGCTAC 
CTCTTGAAGC AGAAAGTTAA AAAGGTTTCA TGTATCTATA AGAAAGACAA AGGCAAAGAA 
GGGGATGGAA CACCTATCAA AGAAGATATC CTCATTGACA AACTGAATGA GAATTCGACG 
CCAGAGAAAA CCGATGTGAC ACCTTCCACC ACTTCTCCAC CTTCGTATGA TAGTGTGACC 
AAACCAGAAA AAGAAAAATT TGAAAAAGAC AAATCAGAAA AGGAAGACAA AGGGAAAGAT 
ATCAGGGAAA GTAAAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC478773 ( XM_535939.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_535939.2 Canis lupus familiaris sodium channel protein type 2 subunit alpha (LOC478773), transcript variant X1, mRNA.

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CloneID	OCe31042
Clone ID Related Accession (Same CDS sequence)	XM_535939.5 , XM_014110824.2 , XM_022414740.1 , XM_014110825.2 , XM_014110824.1 , XM_014110825.1 , XM_535939.6
Accession Version	XM_535939.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	sodium channel protein type 2 subunit alpha
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726122.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_535939.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGT CAGTGCTGGT ACCACCAGGA CCTGACAGCT TCCGCTTCTT TACCAGGGAA 
TCCCTTGCTG CTATTGAACA ACGCATTGCA GAAGAGAAAG CTAAGAGACC CAAACAGGAG 
CGTAAGGATG AGGATGATGA AAATGGCCCA AAGCCAAACA GTGACTTGGA AGCAGGGAAA 
TCTCTTCCAT TTATTTATGG AGACATTCCT CCAGAGATGG TGTCAGAGCC CTTGGAGGAC 
CTGGACCCGT ACTATATCAA TAAGAAAACA TTTATAGTAT TGAATAAAGG GAAAGCAATC 
TCTCGATTCA GTGCCACCCC TGCCCTGTAC ATTTTAACTC CCTTCAACCC TATTAGAAAA 
TTAGCTATTA AGATTTTGGT ACATTCTTTA TTCAATGTGC TCATTATGTG CACTATTCTC 
ACCAACTGTG TATTTATGAC CATGAGTAAC CCTCCAGACT GGACAAAGAA TGTGGAGTAC 
ACCTTTACAG GAATTTATAC ATTTGAATCA CTTATAAAAA TACTTGCAAG GGGCTTTTGT 
TTAGAAGATT TCACATTTCT CCGGGATCCA TGGAATTGGT TGGATTTCAC AGTCATTACT 
TTTGCATACG TGACAGAGTT TGTGGATCTG GGCAATGTCT CAGCGCTGAG AACATTCAGA 
GTTCTCCGAG CACTGAAAAC AATTTCAGTC ATTCCAGGCC TGAAGACCAT TGTGGGGGCC 
CTGATCCAGT CGGTGAAGAA GCTCTCTGAT GTAATGATTC TGACTGTGTT CTGTCTGAGT 
GTGTTTGCAC TCATAGGACT GCAGCTGTTT ATGGGCAACC TGCGGAATAA ATGCTTGCAA 
TGGCCCCCAG ATAATTCTTC CTTTGAAATA AACATCACTT CCTTCTTTAA TAATTCATTG 
GATGGGAACG GTACCATGTT CAATAGGACA GTGAGCATGT TTAACTGGGA TGAATATATT 
GAGGATAAAA GTCACTTTTA TTTTTTGGAA GGGCAAAATG ATGCTCTGCT TTGTGGCAAC 
AGCTCAGATG CAGGCCAGTG TCCGGAAGGA TATATCTGTG TGAAGGCTGG TAGAAACCCC 
AACTATGGCT ATACAAGCTT TGATACCTTT AGTTGGGCCT TCTTGTCATT ATTTCGTCTC 
ATGACTCAAG ACTTCTGGGA AAACCTTTAC CAACTGACGC TACGTGCTGC TGGGAAAACA 
TATATGATAT TTTTTGTGCT GGTCATTTTC TTGGGCTCAT TCTACCTTAT AAATTTAATC 
CTGGCTGTGG TGGCTATGGC CTACGAGGAA CAGAATCAGG CAACTCTTGA GGAAGCTGAA 
CAGAAAGAAG CTGAATTTCA GCAGATGCTC GAACAACTAA AAAAACAACA AGAAGAAGCT 
CAGGCAGCAG CTGCAGCCGC ATCTGCAGAA TCAAGAGAGT TCAGTGGTGC TGGTGGGATA 
GGAGTTTTCT CAGAGAGTTC TTCAGTAGCA TCAAAGTTGA GCTCCAAGAG TGAAAAAGAG 
CTGAAAAACA GAAGGAAGAA AAAGAAGCAG AAAGAGCAGT CTGGAGAAGA AGAGAAGGAA 
GATGGGGTTC GCAAATCCGA ATCCGAAGAC AGTATAAGAA GAAAAGGTTT CCGTTTTTCC 
TTAGAAGGAA GCAGGCTGAC ATATGAAAAA AGGTTCTCTT CTCCACATCA GTCTTTATTG 
AGCATCCGTG GCTCCCTTTT CTCTCCAAGA CGCAACAGTA GGGCGAGCCT TTTCAGTTTC 
AGAGGCCGAA CAAAGGATAT TGGCTCTGAG AACGACTTTG CTGATGATGA GCACAGCACC 
TTTGAAGACA ATGACAGCCG GAGAGACTCT CTGTTTGTGC CACACAGACA TGGAGAGCGG 
CGTCATAGCA ATGTCAGCCA GGCCAGCCGG GCCTCCAGGG TGATCCCCAT CCTGCCGGTG 
AATGGAAAGA TGCACAGTGC AGTGGATTGC AACGGGGTCG TCTCGCTGGT TGGGGGCCCT 
TCTGCCCTCA CGTCTCCAGC TGGACAGCTC CTTCCGGAGG GCACGACTAC TGAAACAGAA 
CTAAGAAAGA GACGGTCCAG TTCTTACCAT GTTTCCATGG ATTTGCTGGA AGATCCTACC 
ACAAGGCAAA GAGCAATGAG TCTAGCCAGT ATTTTGACCA ACACCATGGA AGAACTTGAA 
GAATCCAGAC AGAAATGCCC ACCCTGTTGG TATAAATTTG CTAACATGTG TTTGATTTGG 
GACTGCTGTA AACCATGGTT AAAGGTGAAA CATCTCGTCA ACCTGGTTGT AATGGACCCG 
TTTGTTGACC TGGCCATCAC CATCTGCATT GTCCTAAATA CACTGTTTAT GGCCATGGAG 
CACTATCCTA TGACAGAGCA GTTCAGCAGT GTGCTGTCAG TTGGGAACCT GGTCTTCACT 
GGGATCTTCA CAGCAGAAAT GTTCCTCAAG ATAATAGCCA TGGATCCATA TTATTACTTT 
CAAGAGGGCT GGAATATTTT TGATGGTTTA ATCGTAAGCC TTAGTTTAAT GGAACTTGGT 
TTGGCAAACG TTGAAGGATT GTCAGTTCTC CGATCATTTC GACTGCTCCG AGTTTTCAAA 
TTGGCAAAAT CCTGGCCCAC CCTGAATATG CTGATAAAGA TCATCGGCAA CTCAGTGGGG 
GCTCTGGGCA ACCTCACCTT GGTGTTGGCC ATCATCGTCT TCATTTTTGC CGTGGTTGGC 
ATGCAGCTCT TTGGTAAGAG CTACAAAGAA TGTGTCTGCA AGATCTCCAA TGATTGTGAG 
CTCCCACGGT GGCACATGCA TGACTTCTTC CACTCCTTCC TGATTGTATT CCGTGTGTTG 
TGTGGAGAGT GGATAGAAAC CATGTGGGAC TGTATGGAGG TCGCTGGCCA AACCATGTGC 
CTTACTGTCT TCATGATGGT CATGGTGATT GGAAACCTGG TGGTTCTGAA TCTCTTTCTG 
GCCTTGCTCT TGAGTTCATT CAGTTCTGAC AACCTTGCAG CCACTGACGA TGATAATGAA 
ATGAACAATC TCCAGATTGC TGTGGGAAGG ATGCAGAAAG GAATCGATTA TGTTAAAAGA 
AAACTACGTG AATTTATTCA GAAAGCCTTT GTTAGAAAGC AGAAAGCCTT AGATGAAATT 
AAACCTCTTG AAGATCTAAA TAACAAAAAA GACAGCTGTA TTTCCAACCA TACAACTATA 
GAAATAGGAA AAGATCTCAA TTATCTCAAA GATGGAAATG GAACTACCAG TGGCATAGGC 
AGCAGTGTGG AAAAATATGT TGTGGATGAA AGTGATTACA TGTCATTTAT AAACAATCCC 
AGCCTCACTG TGACTGTACC AATTGCTGTC GGGGAATCTG ACTTTGAAAA TTTAAATACT 
GAAGAATTTA GCAGCGAGTC AGATATGGAG GAAAGCAAAG AGAAGCTAAA TGCAACTAGT 
TCATCTGAAG GCAGCACAGT TGATATAGGA GCTCCCGCTG AGGGAGAGCA ACCTGAGGTA 
GAACCCGAGG AATCTCTTGA ACCTGAAGCC TGTTTCACGG AAGACTGTGT ACGGAAGTTC 
AAGTGTTGTC AGATAAGCAT AGAAGAAGGC AAAGGGAAGC TCTGGTGGAA CTTGAGAAAA 
ACTTGCTATA AGATAGTGGA GCACAACTGG TTTGAAACTT TCATTGTCTT CATGATTCTT 
CTCAGCAGTG GGGCACTGGC CTTTGAAGAT ATATATATTG AGCAGCGAAA AACCATTAAG 
ACCATGTTAG AATATGCCGA CAAAGTTTTC ACTTACATAT TCATTCTGGA AATGCTTCTC 
AAGTGGGTCG CATATGGTTT TCAAATGTAT TTCACCAATG CCTGGTGCTG GCTGGATTTC 
CTGATTGTTG ACGTCTCATT GGTTAGCTTG ACTGCAAACG CCTTGGGTTA CTCAGAACTT 
GGCGCCATCA AATCCCTCAG GACACTCAGA GCTCTGAGGC CATTGAGAGC CCTATCCCGG 
TTTGAAGGAA TGAGGGTTGT TGTCAATGCT CTTCTAGGAG CCATTCCATC CATAATGAAT 
GTGCTCCTGG TTTGTCTTAT CTTTTGGCTA ATATTCAGCA TCATGGGAGT AAATCTTTTC 
GCTGGCAAGT TTTACCATTG TATTAACTAC ACCACTGGAG AAATGTTTGA TGTAAGTGTG 
GTCAACAACT ACAGCGAATG TAAAGCCCTC ATAGAGAGCA ATCAAACTGC CAGGTGGAAA 
AATGTGAAAG TAAACTTTGA TAATGTAGGA CTTGGATATC TTTCTCTGCT TCAAGTAGCC 
ACATTTAAGG GATGGATGGA CATCATGTAT GCAGCTGTTG ATTCACGAAA TGTAGAATTA 
CAACCCAAGT ATGAAGACAA TCTGTACATG TACCTTTATT TTGTCATCTT TATTATTTTT 
GGTTCATTCT TTACCCTGAA TCTTTTCATT GGTGTCATCA TAGATAACTT CAACCAACAG 
AAAAAGAAGT TTGGAGGTCA AGACATTTTT ATGACAGAAG AGCAGAAGAA ATACTACAAT 
GCAATGAAAA AACTGGGTTC AAAGAAACCA CAAAAACCCA TACCTCGACC TGCTAATAAA 
TTCCAAGGAA TGGTCTTTGA TTTTGTAACC AAACAAGTCT TTGATATCAG TATCATGATC 
CTCATCTGCC TCAACATGGT CACCATGATG GTGGAAACTG ATGACCAGAG TCAGGAGATG 
ACAAACATTC TGTACTGGAT TAATCTGGTG TTTATTGTTC TGTTCACTGG AGAATGTGTG 
CTGAAATTAA TCTCCCTTCG TTATTACTAT TTCACCATTG GATGGAATAT TTTTGATTTT 
GTGGTGGTCA TTCTCTCTAT CGTAGGCATG TTTCTGGCTG AGCTGATAGA AAAATACTTT 
GTGTCCCCCA CCCTGTTCCG AGTGATCCGG CTCGCCAGGA TCGGTCGAAT CTTGCGTCTG 
ATCAAAGGGG CGAAGGGGAT CCGCACACTG CTCTTTGCTC TGATGATGTC CCTCCCTGCC 
TTGTTTAACA TCGGCCTCCT GCTCTTCCTG GTCATGTTCA TCTACGCCAT CTTTGGAATG 
TCCAACTTTG CCTATGTCAA GAGGGAAGTT GGAATTGATG ACATGTTCAA CTTTGAGACC 
TTTGGCAACA GCATGATCTG CTTGTTCCAA ATTACCACCT CGGCTGGCTG GGATGGATTG 
CTAGCACCTA TTCTTAATAG TGGACCTCCA GACTGTGATC CTGAAAAAGA TCACCCTGGA 
AGCTCAGTTA AGGGAGACTG TGGGAACCCA TCTGTGGGGA TTTTCTTTTT TGTCAGTTAC 
ATCATCATCT CCTTTCTGGT TGTGGTGAAC ATGTACATCG CTGTCATCCT GGAGAACTTC 
AGTGTTGCCA CTGAGGAGAG TGCAGAGCCC CTGAGCGAGG ATGACTTTGA GATGTTCTAT 
GAAGTCTGGG AGAAGTTTGA TCCTGATGCC ACCCAGTTTA TAGAGTTCTC CAAGCTCTCT 
GATTTTGCAG CTGCCCTGGA TCCTCCTCTT CTCATAGCGA AACCAAACAA AGTCCAGCTC 
ATTGCCATGG ACCTGCCCAT GGTCAGTGGG GACCGGATCC ACTGCCTCGA CATTTTATTT 
GCCTTTACAA AGCGTGTTTT GGGTGAGAGT GGAGAGATGG ATGCCCTTAG AATACAGATG 
GAGGAGCGAT TCATGGCATC GAACCCCTCC AAAGTCTCCT ATGAACCCAT CACAACCACT 
CTGAAACGCA AACAAGAGGA GGTGTCTGCT ATCGTTATCC AGAGGGCTTA TAGACGCTAC 
CTCTTGAAGC AGAAAGTTAA AAAGGTTTCA TGTATCTATA AGAAAGACAA AGGCAAAGAA 
GGGGATGGAA CACCTATCAA AGAAGATATC CTCATTGACA AACTGAATGA GAATTCGACG 
CCAGAGAAAA CCGATGTGAC ACCTTCCACC ACTTCTCCAC CTTCGTATGA TAGTGTGACC 
AAACCAGAAA AAGAAAAATT TGAAAAAGAC AAATCAGAAA AGGAAGACAA AGGGAAAGAT 
ATCAGGGAAA GTAAAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_535939.2
CDS	168..6188
Translation	MAQSVLVPPGPDSFRFFTRESLAAIEQRIAEEKAKRPKQERKDEDDENGPKPNSDLEAGKSLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAISRFSATPALYILTPFNPIRKLAIKILVHSLFNVLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKILARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCLQWPPDNSSFEINITSFFNNSLDGNGTMFNRTVSMFNWDEYIEDKSHFYFLEGQNDALLCGNSSDAGQCPEGYICVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMLEQLKKQQEEAQAAAAAASAESREFSGAGGIGVFSESSSVASKLSSKSEKELKNRRKKKKQKEQSGEEEKEDGVRKSESEDSIRRKGFRFSLEGSRLTYEKRFSSPHQSLLSIRGSLFSPRRNSRASLFSFRGRTKDIGSENDFADDEHSTFEDNDSRRDSLFVPHRHGERRHSNVSQASRASRVIPILPVNGKMHSAVDCNGVVSLVGGPSALTSPAGQLLPEGTTTETELRKRRSSSYHVSMDLLEDPTTRQRAMSLASILTNTMEELEESRQKCPPCWYKFANMCLIWDCCKPWLKVKHLVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTEQFSSVLSVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGLIVSLSLMELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKISNDCELPRWHMHDFFHSFLIVFRVLCGEWIETMWDCMEVAGQTMCLTVFMMVMVIGNLVVLNLFLALLLSSFSSDNLAATDDDNEMNNLQIAVGRMQKGIDYVKRKLREFIQKAFVRKQKALDEIKPLEDLNNKKDSCISNHTTIEIGKDLNYLKDGNGTTSGIGSSVEKYVVDESDYMSFINNPSLTVTVPIAVGESDFENLNTEEFSSESDMEESKEKLNATSSSEGSTVDIGAPAEGEQPEVEPEESLEPEACFTEDCVRKFKCCQISIEEGKGKLWWNLRKTCYKIVEHNWFETFIVFMILLSSGALAFEDIYIEQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGFQMYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINYTTGEMFDVSVVNNYSECKALIESNQTARWKNVKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEDNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPANKFQGMVFDFVTKQVFDISIMILICLNMVTMMVETDDQSQEMTNILYWINLVFIVLFTGECVLKLISLRYYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSGPPDCDPEKDHPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFIEFSKLSDFAAALDPPLLIAKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYEPITTTLKRKQEEVSAIVIQRAYRRYLLKQKVKKVSCIYKKDKGKEGDGTPIKEDILIDKLNENSTPEKTDVTPSTTSPPSYDSVTKPEKEKFEKDKSEKEDKGKDIRESKK

Target ORF information:

RefSeq Version	XM_535939.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris sodium channel protein type 2 subunit alpha (LOC478773), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_535939.5

ORF Insert Sequence:

ATGGCACAGT CAGTGCTGGT ACCACCAGGA CCTGACAGCT TCCGCTTCTT TACCAGGGAA 
TCCCTTGCTG CTATTGAACA ACGCATTGCA GAAGAGAAAG CTAAGAGACC CAAACAGGAG 
CGTAAGGATG AGGATGATGA AAATGGCCCA AAGCCAAACA GTGACTTGGA AGCAGGGAAA 
TCTCTTCCAT TTATTTATGG AGACATTCCT CCAGAGATGG TGTCAGAGCC CTTGGAGGAC 
CTGGACCCGT ACTATATCAA TAAGAAAACA TTTATAGTAT TGAATAAAGG GAAAGCAATC 
TCTCGATTCA GTGCCACCCC TGCCCTGTAC ATTTTAACTC CCTTCAACCC TATTAGAAAA 
TTAGCTATTA AGATTTTGGT ACATTCTTTA TTCAATGTGC TCATTATGTG CACTATTCTC 
ACCAACTGTG TATTTATGAC CATGAGTAAC CCTCCAGACT GGACAAAGAA TGTGGAGTAC 
ACCTTTACAG GAATTTATAC ATTTGAATCA CTTATAAAAA TACTTGCAAG GGGCTTTTGT 
TTAGAAGATT TCACATTTCT CCGGGATCCA TGGAATTGGT TGGATTTCAC AGTCATTACT 
TTTGCATACG TGACAGAGTT TGTGGATCTG GGCAATGTCT CAGCGCTGAG AACATTCAGA 
GTTCTCCGAG CACTGAAAAC AATTTCAGTC ATTCCAGGCC TGAAGACCAT TGTGGGGGCC 
CTGATCCAGT CGGTGAAGAA GCTCTCTGAT GTAATGATTC TGACTGTGTT CTGTCTGAGT 
GTGTTTGCAC TCATAGGACT GCAGCTGTTT ATGGGCAACC TGCGGAATAA ATGCTTGCAA 
TGGCCCCCAG ATAATTCTTC CTTTGAAATA AACATCACTT CCTTCTTTAA TAATTCATTG 
GATGGGAACG GTACCATGTT CAATAGGACA GTGAGCATGT TTAACTGGGA TGAATATATT 
GAGGATAAAA GTCACTTTTA TTTTTTGGAA GGGCAAAATG ATGCTCTGCT TTGTGGCAAC 
AGCTCAGATG CAGGCCAGTG TCCGGAAGGA TATATCTGTG TGAAGGCTGG TAGAAACCCC 
AACTATGGCT ATACAAGCTT TGATACCTTT AGTTGGGCCT TCTTGTCATT ATTTCGTCTC 
ATGACTCAAG ACTTCTGGGA AAACCTTTAC CAACTGACGC TACGTGCTGC TGGGAAAACA 
TATATGATAT TTTTTGTGCT GGTCATTTTC TTGGGCTCAT TCTACCTTAT AAATTTAATC 
CTGGCTGTGG TGGCTATGGC CTACGAGGAA CAGAATCAGG CAACTCTTGA GGAAGCTGAA 
CAGAAAGAAG CTGAATTTCA GCAGATGCTC GAACAACTAA AAAAACAACA AGAAGAAGCT 
CAGGCAGCAG CTGCAGCCGC ATCTGCAGAA TCAAGAGAGT TCAGTGGTGC TGGTGGGATA 
GGAGTTTTCT CAGAGAGTTC TTCAGTAGCA TCAAAGTTGA GCTCCAAGAG TGAAAAAGAG 
CTGAAAAACA GAAGGAAGAA AAAGAAGCAG AAAGAGCAGT CTGGAGAAGA AGAGAAGGAA 
GATGGGGTTC GCAAATCCGA ATCCGAAGAC AGTATAAGAA GAAAAGGTTT CCGTTTTTCC 
TTAGAAGGAA GCAGGCTGAC ATATGAAAAA AGGTTCTCTT CTCCACATCA GTCTTTATTG 
AGCATCCGTG GCTCCCTTTT CTCTCCAAGA CGCAACAGTA GGGCGAGCCT TTTCAGTTTC 
AGAGGCCGAA CAAAGGATAT TGGCTCTGAG AACGACTTTG CTGATGATGA GCACAGCACC 
TTTGAAGACA ATGACAGCCG GAGAGACTCT CTGTTTGTGC CACACAGACA TGGAGAGCGG 
CGTCATAGCA ATGTCAGCCA GGCCAGCCGG GCCTCCAGGG TGATCCCCAT CCTGCCGGTG 
AATGGAAAGA TGCACAGTGC AGTGGATTGC AACGGGGTCG TCTCGCTGGT TGGGGGCCCT 
TCTGCCCTCA CGTCTCCAGC TGGACAGCTC CTTCCGGAGG GCACGACTAC TGAAACAGAA 
CTAAGAAAGA GACGGTCCAG TTCTTACCAT GTTTCCATGG ATTTGCTGGA AGATCCTACC 
ACAAGGCAAA GAGCAATGAG TCTAGCCAGT ATTTTGACCA ACACCATGGA AGAACTTGAA 
GAATCCAGAC AGAAATGCCC ACCCTGTTGG TATAAATTTG CTAACATGTG TTTGATTTGG 
GACTGCTGTA AACCATGGTT AAAGGTGAAA CATCTCGTCA ACCTGGTTGT AATGGACCCG 
TTTGTTGACC TGGCCATCAC CATCTGCATT GTCCTAAATA CACTGTTTAT GGCCATGGAG 
CACTATCCTA TGACAGAGCA GTTCAGCAGT GTGCTGTCAG TTGGGAACCT GGTCTTCACT 
GGGATCTTCA CAGCAGAAAT GTTCCTCAAG ATAATAGCCA TGGATCCATA TTATTACTTT 
CAAGAGGGCT GGAATATTTT TGATGGTTTA ATCGTAAGCC TTAGTTTAAT GGAACTTGGT 
TTGGCAAACG TTGAAGGATT GTCAGTTCTC CGATCATTTC GACTGCTCCG AGTTTTCAAA 
TTGGCAAAAT CCTGGCCCAC CCTGAATATG CTGATAAAGA TCATCGGCAA CTCAGTGGGG 
GCTCTGGGCA ACCTCACCTT GGTGTTGGCC ATCATCGTCT TCATTTTTGC CGTGGTTGGC 
ATGCAGCTCT TTGGTAAGAG CTACAAAGAA TGTGTCTGCA AGATCTCCAA TGATTGTGAG 
CTCCCACGGT GGCACATGCA TGACTTCTTC CACTCCTTCC TGATTGTATT CCGTGTGTTG 
TGTGGAGAGT GGATAGAAAC CATGTGGGAC TGTATGGAGG TCGCTGGCCA AACCATGTGC 
CTTACTGTCT TCATGATGGT CATGGTGATT GGAAACCTGG TGGTTCTGAA TCTCTTTCTG 
GCCTTGCTCT TGAGTTCATT CAGTTCTGAC AACCTTGCAG CCACTGACGA TGATAATGAA 
ATGAACAATC TCCAGATTGC TGTGGGAAGG ATGCAGAAAG GAATCGATTA TGTTAAAAGA 
AAACTACGTG AATTTATTCA GAAAGCCTTT GTTAGAAAGC AGAAAGCCTT AGATGAAATT 
AAACCTCTTG AAGATCTAAA TAACAAAAAA GACAGCTGTA TTTCCAACCA TACAACTATA 
GAAATAGGAA AAGATCTCAA TTATCTCAAA GATGGAAATG GAACTACCAG TGGCATAGGC 
AGCAGTGTGG AAAAATATGT TGTGGATGAA AGTGATTACA TGTCATTTAT AAACAATCCC 
AGCCTCACTG TGACTGTACC AATTGCTGTC GGGGAATCTG ACTTTGAAAA TTTAAATACT 
GAAGAATTTA GCAGCGAGTC AGATATGGAG GAAAGCAAAG AGAAGCTAAA TGCAACTAGT 
TCATCTGAAG GCAGCACAGT TGATATAGGA GCTCCCGCTG AGGGAGAGCA ACCTGAGGTA 
GAACCCGAGG AATCTCTTGA ACCTGAAGCC TGTTTCACGG AAGACTGTGT ACGGAAGTTC 
AAGTGTTGTC AGATAAGCAT AGAAGAAGGC AAAGGGAAGC TCTGGTGGAA CTTGAGAAAA 
ACTTGCTATA AGATAGTGGA GCACAACTGG TTTGAAACTT TCATTGTCTT CATGATTCTT 
CTCAGCAGTG GGGCACTGGC CTTTGAAGAT ATATATATTG AGCAGCGAAA AACCATTAAG 
ACCATGTTAG AATATGCCGA CAAAGTTTTC ACTTACATAT TCATTCTGGA AATGCTTCTC 
AAGTGGGTCG CATATGGTTT TCAAATGTAT TTCACCAATG CCTGGTGCTG GCTGGATTTC 
CTGATTGTTG ACGTCTCATT GGTTAGCTTG ACTGCAAACG CCTTGGGTTA CTCAGAACTT 
GGCGCCATCA AATCCCTCAG GACACTCAGA GCTCTGAGGC CATTGAGAGC CCTATCCCGG 
TTTGAAGGAA TGAGGGTTGT TGTCAATGCT CTTCTAGGAG CCATTCCATC CATAATGAAT 
GTGCTCCTGG TTTGTCTTAT CTTTTGGCTA ATATTCAGCA TCATGGGAGT AAATCTTTTC 
GCTGGCAAGT TTTACCATTG TATTAACTAC ACCACTGGAG AAATGTTTGA TGTAAGTGTG 
GTCAACAACT ACAGCGAATG TAAAGCCCTC ATAGAGAGCA ATCAAACTGC CAGGTGGAAA 
AATGTGAAAG TAAACTTTGA TAATGTAGGA CTTGGATATC TTTCTCTGCT TCAAGTAGCC 
ACATTTAAGG GATGGATGGA CATCATGTAT GCAGCTGTTG ATTCACGAAA TGTAGAATTA 
CAACCCAAGT ATGAAGACAA TCTGTACATG TACCTTTATT TTGTCATCTT TATTATTTTT 
GGTTCATTCT TTACCCTGAA TCTTTTCATT GGTGTCATCA TAGATAACTT CAACCAACAG 
AAAAAGAAGT TTGGAGGTCA AGACATTTTT ATGACAGAAG AGCAGAAGAA ATACTACAAT 
GCAATGAAAA AACTGGGTTC AAAGAAACCA CAAAAACCCA TACCTCGACC TGCTAATAAA 
TTCCAAGGAA TGGTCTTTGA TTTTGTAACC AAACAAGTCT TTGATATCAG TATCATGATC 
CTCATCTGCC TCAACATGGT CACCATGATG GTGGAAACTG ATGACCAGAG TCAGGAGATG 
ACAAACATTC TGTACTGGAT TAATCTGGTG TTTATTGTTC TGTTCACTGG AGAATGTGTG 
CTGAAATTAA TCTCCCTTCG TTATTACTAT TTCACCATTG GATGGAATAT TTTTGATTTT 
GTGGTGGTCA TTCTCTCTAT CGTAGGCATG TTTCTGGCTG AGCTGATAGA AAAATACTTT 
GTGTCCCCCA CCCTGTTCCG AGTGATCCGG CTCGCCAGGA TCGGTCGAAT CTTGCGTCTG 
ATCAAAGGGG CGAAGGGGAT CCGCACACTG CTCTTTGCTC TGATGATGTC CCTCCCTGCC 
TTGTTTAACA TCGGCCTCCT GCTCTTCCTG GTCATGTTCA TCTACGCCAT CTTTGGAATG 
TCCAACTTTG CCTATGTCAA GAGGGAAGTT GGAATTGATG ACATGTTCAA CTTTGAGACC 
TTTGGCAACA GCATGATCTG CTTGTTCCAA ATTACCACCT CGGCTGGCTG GGATGGATTG 
CTAGCACCTA TTCTTAATAG TGGACCTCCA GACTGTGATC CTGAAAAAGA TCACCCTGGA 
AGCTCAGTTA AGGGAGACTG TGGGAACCCA TCTGTGGGGA TTTTCTTTTT TGTCAGTTAC 
ATCATCATCT CCTTTCTGGT TGTGGTGAAC ATGTACATCG CTGTCATCCT GGAGAACTTC 
AGTGTTGCCA CTGAGGAGAG TGCAGAGCCC CTGAGCGAGG ATGACTTTGA GATGTTCTAT 
GAAGTCTGGG AGAAGTTTGA TCCTGATGCC ACCCAGTTTA TAGAGTTCTC CAAGCTCTCT 
GATTTTGCAG CTGCCCTGGA TCCTCCTCTT CTCATAGCGA AACCAAACAA AGTCCAGCTC 
ATTGCCATGG ACCTGCCCAT GGTCAGTGGG GACCGGATCC ACTGCCTCGA CATTTTATTT 
GCCTTTACAA AGCGTGTTTT GGGTGAGAGT GGAGAGATGG ATGCCCTTAG AATACAGATG 
GAGGAGCGAT TCATGGCATC GAACCCCTCC AAAGTCTCCT ATGAACCCAT CACAACCACT 
CTGAAACGCA AACAAGAGGA GGTGTCTGCT ATCGTTATCC AGAGGGCTTA TAGACGCTAC 
CTCTTGAAGC AGAAAGTTAA AAAGGTTTCA TGTATCTATA AGAAAGACAA AGGCAAAGAA 
GGGGATGGAA CACCTATCAA AGAAGATATC CTCATTGACA AACTGAATGA GAATTCGACG 
CCAGAGAAAA CCGATGTGAC ACCTTCCACC ACTTCTCCAC CTTCGTATGA TAGTGTGACC 
AAACCAGAAA AAGAAAAATT TGAAAAAGAC AAATCAGAAA AGGAAGACAA AGGGAAAGAT 
ATCAGGGAAA GTAAAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC478773 ( XM_014110824.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013966299.1 Canis lupus familiaris sodium channel protein type 2 subunit alpha (LOC478773), transcript variant X2, mRNA.

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CloneID	OCe31042
Clone ID Related Accession (Same CDS sequence)	XM_535939.5 , XM_014110824.2 , XM_022414740.1 , XM_014110825.2 , XM_014110824.1 , XM_014110825.1 , XM_535939.6
Accession Version	XM_014110824.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	sodium channel protein type 2 subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006618.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014110824.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGT CAGTGCTGGT ACCACCAGGA CCTGACAGCT TCCGCTTCTT TACCAGGGAA 
TCCCTTGCTG CTATTGAACA ACGCATTGCA GAAGAGAAAG CTAAGAGACC CAAACAGGAG 
CGTAAGGATG AGGATGATGA AAATGGCCCA AAGCCAAACA GTGACTTGGA AGCAGGGAAA 
TCTCTTCCAT TTATTTATGG AGACATTCCT CCAGAGATGG TGTCAGAGCC CTTGGAGGAC 
CTGGACCCGT ACTATATCAA TAAGAAAACA TTTATAGTAT TGAATAAAGG GAAAGCAATC 
TCTCGATTCA GTGCCACCCC TGCCCTGTAC ATTTTAACTC CCTTCAACCC TATTAGAAAA 
TTAGCTATTA AGATTTTGGT ACATTCTTTA TTCAATGTGC TCATTATGTG CACTATTCTC 
ACCAACTGTG TATTTATGAC CATGAGTAAC CCTCCAGACT GGACAAAGAA TGTGGAGTAC 
ACCTTTACAG GAATTTATAC ATTTGAATCA CTTATAAAAA TACTTGCAAG GGGCTTTTGT 
TTAGAAGATT TCACATTTCT CCGGGATCCA TGGAATTGGT TGGATTTCAC AGTCATTACT 
TTTGCATACG TGACAGAGTT TGTGGATCTG GGCAATGTCT CAGCGCTGAG AACATTCAGA 
GTTCTCCGAG CACTGAAAAC AATTTCAGTC ATTCCAGGCC TGAAGACCAT TGTGGGGGCC 
CTGATCCAGT CGGTGAAGAA GCTCTCTGAT GTAATGATTC TGACTGTGTT CTGTCTGAGT 
GTGTTTGCAC TCATAGGACT GCAGCTGTTT ATGGGCAACC TGCGGAATAA ATGCTTGCAA 
TGGCCCCCAG ATAATTCTTC CTTTGAAATA AACATCACTT CCTTCTTTAA TAATTCATTG 
GATGGGAACG GTACCATGTT CAATAGGACA GTGAGCATGT TTAACTGGGA TGAATATATT 
GAGGATAAAA GTCACTTTTA TTTTTTGGAA GGGCAAAATG ATGCTCTGCT TTGTGGCAAC 
AGCTCAGATG CAGGCCAGTG TCCGGAAGGA TATATCTGTG TGAAGGCTGG TAGAAACCCC 
AACTATGGCT ATACAAGCTT TGATACCTTT AGTTGGGCCT TCTTGTCATT ATTTCGTCTC 
ATGACTCAAG ACTTCTGGGA AAACCTTTAC CAACTGACGC TACGTGCTGC TGGGAAAACA 
TATATGATAT TTTTTGTGCT GGTCATTTTC TTGGGCTCAT TCTACCTTAT AAATTTAATC 
CTGGCTGTGG TGGCTATGGC CTACGAGGAA CAGAATCAGG CAACTCTTGA GGAAGCTGAA 
CAGAAAGAAG CTGAATTTCA GCAGATGCTC GAACAACTAA AAAAACAACA AGAAGAAGCT 
CAGGCAGCAG CTGCAGCCGC ATCTGCAGAA TCAAGAGAGT TCAGTGGTGC TGGTGGGATA 
GGAGTTTTCT CAGAGAGTTC TTCAGTAGCA TCAAAGTTGA GCTCCAAGAG TGAAAAAGAG 
CTGAAAAACA GAAGGAAGAA AAAGAAGCAG AAAGAGCAGT CTGGAGAAGA AGAGAAGGAA 
GATGGGGTTC GCAAATCCGA ATCCGAAGAC AGTATAAGAA GAAAAGGTTT CCGTTTTTCC 
TTAGAAGGAA GCAGGCTGAC ATATGAAAAA AGGTTCTCTT CTCCACATCA GTCTTTATTG 
AGCATCCGTG GCTCCCTTTT CTCTCCAAGA CGCAACAGTA GGGCGAGCCT TTTCAGTTTC 
AGAGGCCGAA CAAAGGATAT TGGCTCTGAG AACGACTTTG CTGATGATGA GCACAGCACC 
TTTGAAGACA ATGACAGCCG GAGAGACTCT CTGTTTGTGC CACACAGACA TGGAGAGCGG 
CGTCATAGCA ATGTCAGCCA GGCCAGCCGG GCCTCCAGGG TGATCCCCAT CCTGCCGGTG 
AATGGAAAGA TGCACAGTGC AGTGGATTGC AACGGGGTCG TCTCGCTGGT TGGGGGCCCT 
TCTGCCCTCA CGTCTCCAGC TGGACAGCTC CTTCCGGAGG GCACGACTAC TGAAACAGAA 
CTAAGAAAGA GACGGTCCAG TTCTTACCAT GTTTCCATGG ATTTGCTGGA AGATCCTACC 
ACAAGGCAAA GAGCAATGAG TCTAGCCAGT ATTTTGACCA ACACCATGGA AGAACTTGAA 
GAATCCAGAC AGAAATGCCC ACCCTGTTGG TATAAATTTG CTAACATGTG TTTGATTTGG 
GACTGCTGTA AACCATGGTT AAAGGTGAAA CATCTCGTCA ACCTGGTTGT AATGGACCCG 
TTTGTTGACC TGGCCATCAC CATCTGCATT GTCCTAAATA CACTGTTTAT GGCCATGGAG 
CACTATCCTA TGACAGAGCA GTTCAGCAGT GTGCTGTCAG TTGGGAACCT GGTCTTCACT 
GGGATCTTCA CAGCAGAAAT GTTCCTCAAG ATAATAGCCA TGGATCCATA TTATTACTTT 
CAAGAGGGCT GGAATATTTT TGATGGTTTA ATCGTAAGCC TTAGTTTAAT GGAACTTGGT 
TTGGCAAACG TTGAAGGATT GTCAGTTCTC CGATCATTTC GACTGCTCCG AGTTTTCAAA 
TTGGCAAAAT CCTGGCCCAC CCTGAATATG CTGATAAAGA TCATCGGCAA CTCAGTGGGG 
GCTCTGGGCA ACCTCACCTT GGTGTTGGCC ATCATCGTCT TCATTTTTGC CGTGGTTGGC 
ATGCAGCTCT TTGGTAAGAG CTACAAAGAA TGTGTCTGCA AGATCTCCAA TGATTGTGAG 
CTCCCACGGT GGCACATGCA TGACTTCTTC CACTCCTTCC TGATTGTATT CCGTGTGTTG 
TGTGGAGAGT GGATAGAAAC CATGTGGGAC TGTATGGAGG TCGCTGGCCA AACCATGTGC 
CTTACTGTCT TCATGATGGT CATGGTGATT GGAAACCTGG TGGTTCTGAA TCTCTTTCTG 
GCCTTGCTCT TGAGTTCATT CAGTTCTGAC AACCTTGCAG CCACTGACGA TGATAATGAA 
ATGAACAATC TCCAGATTGC TGTGGGAAGG ATGCAGAAAG GAATCGATTA TGTTAAAAGA 
AAACTACGTG AATTTATTCA GAAAGCCTTT GTTAGAAAGC AGAAAGCCTT AGATGAAATT 
AAACCTCTTG AAGATCTAAA TAACAAAAAA GACAGCTGTA TTTCCAACCA TACAACTATA 
GAAATAGGAA AAGATCTCAA TTATCTCAAA GATGGAAATG GAACTACCAG TGGCATAGGC 
AGCAGTGTGG AAAAATATGT TGTGGATGAA AGTGATTACA TGTCATTTAT AAACAATCCC 
AGCCTCACTG TGACTGTACC AATTGCTGTC GGGGAATCTG ACTTTGAAAA TTTAAATACT 
GAAGAATTTA GCAGCGAGTC AGATATGGAG GAAAGCAAAG AGAAGCTAAA TGCAACTAGT 
TCATCTGAAG GCAGCACAGT TGATATAGGA GCTCCCGCTG AGGGAGAGCA ACCTGAGGTA 
GAACCCGAGG AATCTCTTGA ACCTGAAGCC TGTTTCACGG AAGACTGTGT ACGGAAGTTC 
AAGTGTTGTC AGATAAGCAT AGAAGAAGGC AAAGGGAAGC TCTGGTGGAA CTTGAGAAAA 
ACTTGCTATA AGATAGTGGA GCACAACTGG TTTGAAACTT TCATTGTCTT CATGATTCTT 
CTCAGCAGTG GGGCACTGGC CTTTGAAGAT ATATATATTG AGCAGCGAAA AACCATTAAG 
ACCATGTTAG AATATGCCGA CAAAGTTTTC ACTTACATAT TCATTCTGGA AATGCTTCTC 
AAGTGGGTCG CATATGGTTT TCAAATGTAT TTCACCAATG CCTGGTGCTG GCTGGATTTC 
CTGATTGTTG ACGTCTCATT GGTTAGCTTG ACTGCAAACG CCTTGGGTTA CTCAGAACTT 
GGCGCCATCA AATCCCTCAG GACACTCAGA GCTCTGAGGC CATTGAGAGC CCTATCCCGG 
TTTGAAGGAA TGAGGGTTGT TGTCAATGCT CTTCTAGGAG CCATTCCATC CATAATGAAT 
GTGCTCCTGG TTTGTCTTAT CTTTTGGCTA ATATTCAGCA TCATGGGAGT AAATCTTTTC 
GCTGGCAAGT TTTACCATTG TATTAACTAC ACCACTGGAG AAATGTTTGA TGTAAGTGTG 
GTCAACAACT ACAGCGAATG TAAAGCCCTC ATAGAGAGCA ATCAAACTGC CAGGTGGAAA 
AATGTGAAAG TAAACTTTGA TAATGTAGGA CTTGGATATC TTTCTCTGCT TCAAGTAGCC 
ACATTTAAGG GATGGATGGA CATCATGTAT GCAGCTGTTG ATTCACGAAA TGTAGAATTA 
CAACCCAAGT ATGAAGACAA TCTGTACATG TACCTTTATT TTGTCATCTT TATTATTTTT 
GGTTCATTCT TTACCCTGAA TCTTTTCATT GGTGTCATCA TAGATAACTT CAACCAACAG 
AAAAAGAAGT TTGGAGGTCA AGACATTTTT ATGACAGAAG AGCAGAAGAA ATACTACAAT 
GCAATGAAAA AACTGGGTTC AAAGAAACCA CAAAAACCCA TACCTCGACC TGCTAATAAA 
TTCCAAGGAA TGGTCTTTGA TTTTGTAACC AAACAAGTCT TTGATATCAG TATCATGATC 
CTCATCTGCC TCAACATGGT CACCATGATG GTGGAAACTG ATGACCAGAG TCAGGAGATG 
ACAAACATTC TGTACTGGAT TAATCTGGTG TTTATTGTTC TGTTCACTGG AGAATGTGTG 
CTGAAATTAA TCTCCCTTCG TTATTACTAT TTCACCATTG GATGGAATAT TTTTGATTTT 
GTGGTGGTCA TTCTCTCTAT CGTAGGCATG TTTCTGGCTG AGCTGATAGA AAAATACTTT 
GTGTCCCCCA CCCTGTTCCG AGTGATCCGG CTCGCCAGGA TCGGTCGAAT CTTGCGTCTG 
ATCAAAGGGG CGAAGGGGAT CCGCACACTG CTCTTTGCTC TGATGATGTC CCTCCCTGCC 
TTGTTTAACA TCGGCCTCCT GCTCTTCCTG GTCATGTTCA TCTACGCCAT CTTTGGAATG 
TCCAACTTTG CCTATGTCAA GAGGGAAGTT GGAATTGATG ACATGTTCAA CTTTGAGACC 
TTTGGCAACA GCATGATCTG CTTGTTCCAA ATTACCACCT CGGCTGGCTG GGATGGATTG 
CTAGCACCTA TTCTTAATAG TGGACCTCCA GACTGTGATC CTGAAAAAGA TCACCCTGGA 
AGCTCAGTTA AGGGAGACTG TGGGAACCCA TCTGTGGGGA TTTTCTTTTT TGTCAGTTAC 
ATCATCATCT CCTTTCTGGT TGTGGTGAAC ATGTACATCG CTGTCATCCT GGAGAACTTC 
AGTGTTGCCA CTGAGGAGAG TGCAGAGCCC CTGAGCGAGG ATGACTTTGA GATGTTCTAT 
GAAGTCTGGG AGAAGTTTGA TCCTGATGCC ACCCAGTTTA TAGAGTTCTC CAAGCTCTCT 
GATTTTGCAG CTGCCCTGGA TCCTCCTCTT CTCATAGCGA AACCAAACAA AGTCCAGCTC 
ATTGCCATGG ACCTGCCCAT GGTCAGTGGG GACCGGATCC ACTGCCTCGA CATTTTATTT 
GCCTTTACAA AGCGTGTTTT GGGTGAGAGT GGAGAGATGG ATGCCCTTAG AATACAGATG 
GAGGAGCGAT TCATGGCATC GAACCCCTCC AAAGTCTCCT ATGAACCCAT CACAACCACT 
CTGAAACGCA AACAAGAGGA GGTGTCTGCT ATCGTTATCC AGAGGGCTTA TAGACGCTAC 
CTCTTGAAGC AGAAAGTTAA AAAGGTTTCA TGTATCTATA AGAAAGACAA AGGCAAAGAA 
GGGGATGGAA CACCTATCAA AGAAGATATC CTCATTGACA AACTGAATGA GAATTCGACG 
CCAGAGAAAA CCGATGTGAC ACCTTCCACC ACTTCTCCAC CTTCGTATGA TAGTGTGACC 
AAACCAGAAA AAGAAAAATT TGAAAAAGAC AAATCAGAAA AGGAAGACAA AGGGAAAGAT 
ATCAGGGAAA GTAAAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013966299.1
CDS	285..6305
Translation	MAQSVLVPPGPDSFRFFTRESLAAIEQRIAEEKAKRPKQERKDEDDENGPKPNSDLEAGKSLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAISRFSATPALYILTPFNPIRKLAIKILVHSLFNVLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKILARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCLQWPPDNSSFEINITSFFNNSLDGNGTMFNRTVSMFNWDEYIEDKSHFYFLEGQNDALLCGNSSDAGQCPEGYICVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMLEQLKKQQEEAQAAAAAASAESREFSGAGGIGVFSESSSVASKLSSKSEKELKNRRKKKKQKEQSGEEEKEDGVRKSESEDSIRRKGFRFSLEGSRLTYEKRFSSPHQSLLSIRGSLFSPRRNSRASLFSFRGRTKDIGSENDFADDEHSTFEDNDSRRDSLFVPHRHGERRHSNVSQASRASRVIPILPVNGKMHSAVDCNGVVSLVGGPSALTSPAGQLLPEGTTTETELRKRRSSSYHVSMDLLEDPTTRQRAMSLASILTNTMEELEESRQKCPPCWYKFANMCLIWDCCKPWLKVKHLVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTEQFSSVLSVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGLIVSLSLMELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKISNDCELPRWHMHDFFHSFLIVFRVLCGEWIETMWDCMEVAGQTMCLTVFMMVMVIGNLVVLNLFLALLLSSFSSDNLAATDDDNEMNNLQIAVGRMQKGIDYVKRKLREFIQKAFVRKQKALDEIKPLEDLNNKKDSCISNHTTIEIGKDLNYLKDGNGTTSGIGSSVEKYVVDESDYMSFINNPSLTVTVPIAVGESDFENLNTEEFSSESDMEESKEKLNATSSSEGSTVDIGAPAEGEQPEVEPEESLEPEACFTEDCVRKFKCCQISIEEGKGKLWWNLRKTCYKIVEHNWFETFIVFMILLSSGALAFEDIYIEQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGFQMYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINYTTGEMFDVSVVNNYSECKALIESNQTARWKNVKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEDNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPANKFQGMVFDFVTKQVFDISIMILICLNMVTMMVETDDQSQEMTNILYWINLVFIVLFTGECVLKLISLRYYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSGPPDCDPEKDHPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFIEFSKLSDFAAALDPPLLIAKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYEPITTTLKRKQEEVSAIVIQRAYRRYLLKQKVKKVSCIYKKDKGKEGDGTPIKEDILIDKLNENSTPEKTDVTPSTTSPPSYDSVTKPEKEKFEKDKSEKEDKGKDIRESKK

Target ORF information:

RefSeq Version	XM_014110824.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris sodium channel protein type 2 subunit alpha (LOC478773), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014110824.2

ORF Insert Sequence:

ATGGCACAGT CAGTGCTGGT ACCACCAGGA CCTGACAGCT TCCGCTTCTT TACCAGGGAA 
TCCCTTGCTG CTATTGAACA ACGCATTGCA GAAGAGAAAG CTAAGAGACC CAAACAGGAG 
CGTAAGGATG AGGATGATGA AAATGGCCCA AAGCCAAACA GTGACTTGGA AGCAGGGAAA 
TCTCTTCCAT TTATTTATGG AGACATTCCT CCAGAGATGG TGTCAGAGCC CTTGGAGGAC 
CTGGACCCGT ACTATATCAA TAAGAAAACA TTTATAGTAT TGAATAAAGG GAAAGCAATC 
TCTCGATTCA GTGCCACCCC TGCCCTGTAC ATTTTAACTC CCTTCAACCC TATTAGAAAA 
TTAGCTATTA AGATTTTGGT ACATTCTTTA TTCAATGTGC TCATTATGTG CACTATTCTC 
ACCAACTGTG TATTTATGAC CATGAGTAAC CCTCCAGACT GGACAAAGAA TGTGGAGTAC 
ACCTTTACAG GAATTTATAC ATTTGAATCA CTTATAAAAA TACTTGCAAG GGGCTTTTGT 
TTAGAAGATT TCACATTTCT CCGGGATCCA TGGAATTGGT TGGATTTCAC AGTCATTACT 
TTTGCATACG TGACAGAGTT TGTGGATCTG GGCAATGTCT CAGCGCTGAG AACATTCAGA 
GTTCTCCGAG CACTGAAAAC AATTTCAGTC ATTCCAGGCC TGAAGACCAT TGTGGGGGCC 
CTGATCCAGT CGGTGAAGAA GCTCTCTGAT GTAATGATTC TGACTGTGTT CTGTCTGAGT 
GTGTTTGCAC TCATAGGACT GCAGCTGTTT ATGGGCAACC TGCGGAATAA ATGCTTGCAA 
TGGCCCCCAG ATAATTCTTC CTTTGAAATA AACATCACTT CCTTCTTTAA TAATTCATTG 
GATGGGAACG GTACCATGTT CAATAGGACA GTGAGCATGT TTAACTGGGA TGAATATATT 
GAGGATAAAA GTCACTTTTA TTTTTTGGAA GGGCAAAATG ATGCTCTGCT TTGTGGCAAC 
AGCTCAGATG CAGGCCAGTG TCCGGAAGGA TATATCTGTG TGAAGGCTGG TAGAAACCCC 
AACTATGGCT ATACAAGCTT TGATACCTTT AGTTGGGCCT TCTTGTCATT ATTTCGTCTC 
ATGACTCAAG ACTTCTGGGA AAACCTTTAC CAACTGACGC TACGTGCTGC TGGGAAAACA 
TATATGATAT TTTTTGTGCT GGTCATTTTC TTGGGCTCAT TCTACCTTAT AAATTTAATC 
CTGGCTGTGG TGGCTATGGC CTACGAGGAA CAGAATCAGG CAACTCTTGA GGAAGCTGAA 
CAGAAAGAAG CTGAATTTCA GCAGATGCTC GAACAACTAA AAAAACAACA AGAAGAAGCT 
CAGGCAGCAG CTGCAGCCGC ATCTGCAGAA TCAAGAGAGT TCAGTGGTGC TGGTGGGATA 
GGAGTTTTCT CAGAGAGTTC TTCAGTAGCA TCAAAGTTGA GCTCCAAGAG TGAAAAAGAG 
CTGAAAAACA GAAGGAAGAA AAAGAAGCAG AAAGAGCAGT CTGGAGAAGA AGAGAAGGAA 
GATGGGGTTC GCAAATCCGA ATCCGAAGAC AGTATAAGAA GAAAAGGTTT CCGTTTTTCC 
TTAGAAGGAA GCAGGCTGAC ATATGAAAAA AGGTTCTCTT CTCCACATCA GTCTTTATTG 
AGCATCCGTG GCTCCCTTTT CTCTCCAAGA CGCAACAGTA GGGCGAGCCT TTTCAGTTTC 
AGAGGCCGAA CAAAGGATAT TGGCTCTGAG AACGACTTTG CTGATGATGA GCACAGCACC 
TTTGAAGACA ATGACAGCCG GAGAGACTCT CTGTTTGTGC CACACAGACA TGGAGAGCGG 
CGTCATAGCA ATGTCAGCCA GGCCAGCCGG GCCTCCAGGG TGATCCCCAT CCTGCCGGTG 
AATGGAAAGA TGCACAGTGC AGTGGATTGC AACGGGGTCG TCTCGCTGGT TGGGGGCCCT 
TCTGCCCTCA CGTCTCCAGC TGGACAGCTC CTTCCGGAGG GCACGACTAC TGAAACAGAA 
CTAAGAAAGA GACGGTCCAG TTCTTACCAT GTTTCCATGG ATTTGCTGGA AGATCCTACC 
ACAAGGCAAA GAGCAATGAG TCTAGCCAGT ATTTTGACCA ACACCATGGA AGAACTTGAA 
GAATCCAGAC AGAAATGCCC ACCCTGTTGG TATAAATTTG CTAACATGTG TTTGATTTGG 
GACTGCTGTA AACCATGGTT AAAGGTGAAA CATCTCGTCA ACCTGGTTGT AATGGACCCG 
TTTGTTGACC TGGCCATCAC CATCTGCATT GTCCTAAATA CACTGTTTAT GGCCATGGAG 
CACTATCCTA TGACAGAGCA GTTCAGCAGT GTGCTGTCAG TTGGGAACCT GGTCTTCACT 
GGGATCTTCA CAGCAGAAAT GTTCCTCAAG ATAATAGCCA TGGATCCATA TTATTACTTT 
CAAGAGGGCT GGAATATTTT TGATGGTTTA ATCGTAAGCC TTAGTTTAAT GGAACTTGGT 
TTGGCAAACG TTGAAGGATT GTCAGTTCTC CGATCATTTC GACTGCTCCG AGTTTTCAAA 
TTGGCAAAAT CCTGGCCCAC CCTGAATATG CTGATAAAGA TCATCGGCAA CTCAGTGGGG 
GCTCTGGGCA ACCTCACCTT GGTGTTGGCC ATCATCGTCT TCATTTTTGC CGTGGTTGGC 
ATGCAGCTCT TTGGTAAGAG CTACAAAGAA TGTGTCTGCA AGATCTCCAA TGATTGTGAG 
CTCCCACGGT GGCACATGCA TGACTTCTTC CACTCCTTCC TGATTGTATT CCGTGTGTTG 
TGTGGAGAGT GGATAGAAAC CATGTGGGAC TGTATGGAGG TCGCTGGCCA AACCATGTGC 
CTTACTGTCT TCATGATGGT CATGGTGATT GGAAACCTGG TGGTTCTGAA TCTCTTTCTG 
GCCTTGCTCT TGAGTTCATT CAGTTCTGAC AACCTTGCAG CCACTGACGA TGATAATGAA 
ATGAACAATC TCCAGATTGC TGTGGGAAGG ATGCAGAAAG GAATCGATTA TGTTAAAAGA 
AAACTACGTG AATTTATTCA GAAAGCCTTT GTTAGAAAGC AGAAAGCCTT AGATGAAATT 
AAACCTCTTG AAGATCTAAA TAACAAAAAA GACAGCTGTA TTTCCAACCA TACAACTATA 
GAAATAGGAA AAGATCTCAA TTATCTCAAA GATGGAAATG GAACTACCAG TGGCATAGGC 
AGCAGTGTGG AAAAATATGT TGTGGATGAA AGTGATTACA TGTCATTTAT AAACAATCCC 
AGCCTCACTG TGACTGTACC AATTGCTGTC GGGGAATCTG ACTTTGAAAA TTTAAATACT 
GAAGAATTTA GCAGCGAGTC AGATATGGAG GAAAGCAAAG AGAAGCTAAA TGCAACTAGT 
TCATCTGAAG GCAGCACAGT TGATATAGGA GCTCCCGCTG AGGGAGAGCA ACCTGAGGTA 
GAACCCGAGG AATCTCTTGA ACCTGAAGCC TGTTTCACGG AAGACTGTGT ACGGAAGTTC 
AAGTGTTGTC AGATAAGCAT AGAAGAAGGC AAAGGGAAGC TCTGGTGGAA CTTGAGAAAA 
ACTTGCTATA AGATAGTGGA GCACAACTGG TTTGAAACTT TCATTGTCTT CATGATTCTT 
CTCAGCAGTG GGGCACTGGC CTTTGAAGAT ATATATATTG AGCAGCGAAA AACCATTAAG 
ACCATGTTAG AATATGCCGA CAAAGTTTTC ACTTACATAT TCATTCTGGA AATGCTTCTC 
AAGTGGGTCG CATATGGTTT TCAAATGTAT TTCACCAATG CCTGGTGCTG GCTGGATTTC 
CTGATTGTTG ACGTCTCATT GGTTAGCTTG ACTGCAAACG CCTTGGGTTA CTCAGAACTT 
GGCGCCATCA AATCCCTCAG GACACTCAGA GCTCTGAGGC CATTGAGAGC CCTATCCCGG 
TTTGAAGGAA TGAGGGTTGT TGTCAATGCT CTTCTAGGAG CCATTCCATC CATAATGAAT 
GTGCTCCTGG TTTGTCTTAT CTTTTGGCTA ATATTCAGCA TCATGGGAGT AAATCTTTTC 
GCTGGCAAGT TTTACCATTG TATTAACTAC ACCACTGGAG AAATGTTTGA TGTAAGTGTG 
GTCAACAACT ACAGCGAATG TAAAGCCCTC ATAGAGAGCA ATCAAACTGC CAGGTGGAAA 
AATGTGAAAG TAAACTTTGA TAATGTAGGA CTTGGATATC TTTCTCTGCT TCAAGTAGCC 
ACATTTAAGG GATGGATGGA CATCATGTAT GCAGCTGTTG ATTCACGAAA TGTAGAATTA 
CAACCCAAGT ATGAAGACAA TCTGTACATG TACCTTTATT TTGTCATCTT TATTATTTTT 
GGTTCATTCT TTACCCTGAA TCTTTTCATT GGTGTCATCA TAGATAACTT CAACCAACAG 
AAAAAGAAGT TTGGAGGTCA AGACATTTTT ATGACAGAAG AGCAGAAGAA ATACTACAAT 
GCAATGAAAA AACTGGGTTC AAAGAAACCA CAAAAACCCA TACCTCGACC TGCTAATAAA 
TTCCAAGGAA TGGTCTTTGA TTTTGTAACC AAACAAGTCT TTGATATCAG TATCATGATC 
CTCATCTGCC TCAACATGGT CACCATGATG GTGGAAACTG ATGACCAGAG TCAGGAGATG 
ACAAACATTC TGTACTGGAT TAATCTGGTG TTTATTGTTC TGTTCACTGG AGAATGTGTG 
CTGAAATTAA TCTCCCTTCG TTATTACTAT TTCACCATTG GATGGAATAT TTTTGATTTT 
GTGGTGGTCA TTCTCTCTAT CGTAGGCATG TTTCTGGCTG AGCTGATAGA AAAATACTTT 
GTGTCCCCCA CCCTGTTCCG AGTGATCCGG CTCGCCAGGA TCGGTCGAAT CTTGCGTCTG 
ATCAAAGGGG CGAAGGGGAT CCGCACACTG CTCTTTGCTC TGATGATGTC CCTCCCTGCC 
TTGTTTAACA TCGGCCTCCT GCTCTTCCTG GTCATGTTCA TCTACGCCAT CTTTGGAATG 
TCCAACTTTG CCTATGTCAA GAGGGAAGTT GGAATTGATG ACATGTTCAA CTTTGAGACC 
TTTGGCAACA GCATGATCTG CTTGTTCCAA ATTACCACCT CGGCTGGCTG GGATGGATTG 
CTAGCACCTA TTCTTAATAG TGGACCTCCA GACTGTGATC CTGAAAAAGA TCACCCTGGA 
AGCTCAGTTA AGGGAGACTG TGGGAACCCA TCTGTGGGGA TTTTCTTTTT TGTCAGTTAC 
ATCATCATCT CCTTTCTGGT TGTGGTGAAC ATGTACATCG CTGTCATCCT GGAGAACTTC 
AGTGTTGCCA CTGAGGAGAG TGCAGAGCCC CTGAGCGAGG ATGACTTTGA GATGTTCTAT 
GAAGTCTGGG AGAAGTTTGA TCCTGATGCC ACCCAGTTTA TAGAGTTCTC CAAGCTCTCT 
GATTTTGCAG CTGCCCTGGA TCCTCCTCTT CTCATAGCGA AACCAAACAA AGTCCAGCTC 
ATTGCCATGG ACCTGCCCAT GGTCAGTGGG GACCGGATCC ACTGCCTCGA CATTTTATTT 
GCCTTTACAA AGCGTGTTTT GGGTGAGAGT GGAGAGATGG ATGCCCTTAG AATACAGATG 
GAGGAGCGAT TCATGGCATC GAACCCCTCC AAAGTCTCCT ATGAACCCAT CACAACCACT 
CTGAAACGCA AACAAGAGGA GGTGTCTGCT ATCGTTATCC AGAGGGCTTA TAGACGCTAC 
CTCTTGAAGC AGAAAGTTAA AAAGGTTTCA TGTATCTATA AGAAAGACAA AGGCAAAGAA 
GGGGATGGAA CACCTATCAA AGAAGATATC CTCATTGACA AACTGAATGA GAATTCGACG 
CCAGAGAAAA CCGATGTGAC ACCTTCCACC ACTTCTCCAC CTTCGTATGA TAGTGTGACC 
AAACCAGAAA AAGAAAAATT TGAAAAAGAC AAATCAGAAA AGGAAGACAA AGGGAAAGAT 
ATCAGGGAAA GTAAAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC478773 ( XM_022414740.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022270448.1 Canis lupus familiaris sodium channel protein type 2 subunit alpha (LOC478773), transcript variant X4, mRNA.

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CloneID	OCe31042
Clone ID Related Accession (Same CDS sequence)	XM_535939.5 , XM_014110824.2 , XM_022414740.1 , XM_014110825.2 , XM_014110824.1 , XM_014110825.1 , XM_535939.6
Accession Version	XM_022414740.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	sodium channel protein type 2 subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006618.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGT CAGTGCTGGT ACCACCAGGA CCTGACAGCT TCCGCTTCTT TACCAGGGAA 
TCCCTTGCTG CTATTGAACA ACGCATTGCA GAAGAGAAAG CTAAGAGACC CAAACAGGAG 
CGTAAGGATG AGGATGATGA AAATGGCCCA AAGCCAAACA GTGACTTGGA AGCAGGGAAA 
TCTCTTCCAT TTATTTATGG AGACATTCCT CCAGAGATGG TGTCAGAGCC CTTGGAGGAC 
CTGGACCCGT ACTATATCAA TAAGAAAACA TTTATAGTAT TGAATAAAGG GAAAGCAATC 
TCTCGATTCA GTGCCACCCC TGCCCTGTAC ATTTTAACTC CCTTCAACCC TATTAGAAAA 
TTAGCTATTA AGATTTTGGT ACATTCTTTA TTCAATGTGC TCATTATGTG CACTATTCTC 
ACCAACTGTG TATTTATGAC CATGAGTAAC CCTCCAGACT GGACAAAGAA TGTGGAGTAC 
ACCTTTACAG GAATTTATAC ATTTGAATCA CTTATAAAAA TACTTGCAAG GGGCTTTTGT 
TTAGAAGATT TCACATTTCT CCGGGATCCA TGGAATTGGT TGGATTTCAC AGTCATTACT 
TTTGCATACG TGACAGAGTT TGTGGATCTG GGCAATGTCT CAGCGCTGAG AACATTCAGA 
GTTCTCCGAG CACTGAAAAC AATTTCAGTC ATTCCAGGCC TGAAGACCAT TGTGGGGGCC 
CTGATCCAGT CGGTGAAGAA GCTCTCTGAT GTAATGATTC TGACTGTGTT CTGTCTGAGT 
GTGTTTGCAC TCATAGGACT GCAGCTGTTT ATGGGCAACC TGCGGAATAA ATGCTTGCAA 
TGGCCCCCAG ATAATTCTTC CTTTGAAATA AACATCACTT CCTTCTTTAA TAATTCATTG 
GATGGGAACG GTACCATGTT CAATAGGACA GTGAGCATGT TTAACTGGGA TGAATATATT 
GAGGATAAAA GTCACTTTTA TTTTTTGGAA GGGCAAAATG ATGCTCTGCT TTGTGGCAAC 
AGCTCAGATG CAGGCCAGTG TCCGGAAGGA TATATCTGTG TGAAGGCTGG TAGAAACCCC 
AACTATGGCT ATACAAGCTT TGATACCTTT AGTTGGGCCT TCTTGTCATT ATTTCGTCTC 
ATGACTCAAG ACTTCTGGGA AAACCTTTAC CAACTGACGC TACGTGCTGC TGGGAAAACA 
TATATGATAT TTTTTGTGCT GGTCATTTTC TTGGGCTCAT TCTACCTTAT AAATTTAATC 
CTGGCTGTGG TGGCTATGGC CTACGAGGAA CAGAATCAGG CAACTCTTGA GGAAGCTGAA 
CAGAAAGAAG CTGAATTTCA GCAGATGCTC GAACAACTAA AAAAACAACA AGAAGAAGCT 
CAGGCAGCAG CTGCAGCCGC ATCTGCAGAA TCAAGAGAGT TCAGTGGTGC TGGTGGGATA 
GGAGTTTTCT CAGAGAGTTC TTCAGTAGCA TCAAAGTTGA GCTCCAAGAG TGAAAAAGAG 
CTGAAAAACA GAAGGAAGAA AAAGAAGCAG AAAGAGCAGT CTGGAGAAGA AGAGAAGGAA 
GATGGGGTTC GCAAATCCGA ATCCGAAGAC AGTATAAGAA GAAAAGGTTT CCGTTTTTCC 
TTAGAAGGAA GCAGGCTGAC ATATGAAAAA AGGTTCTCTT CTCCACATCA GTCTTTATTG 
AGCATCCGTG GCTCCCTTTT CTCTCCAAGA CGCAACAGTA GGGCGAGCCT TTTCAGTTTC 
AGAGGCCGAA CAAAGGATAT TGGCTCTGAG AACGACTTTG CTGATGATGA GCACAGCACC 
TTTGAAGACA ATGACAGCCG GAGAGACTCT CTGTTTGTGC CACACAGACA TGGAGAGCGG 
CGTCATAGCA ATGTCAGCCA GGCCAGCCGG GCCTCCAGGG TGATCCCCAT CCTGCCGGTG 
AATGGAAAGA TGCACAGTGC AGTGGATTGC AACGGGGTCG TCTCGCTGGT TGGGGGCCCT 
TCTGCCCTCA CGTCTCCAGC TGGACAGCTC CTTCCGGAGG GCACGACTAC TGAAACAGAA 
CTAAGAAAGA GACGGTCCAG TTCTTACCAT GTTTCCATGG ATTTGCTGGA AGATCCTACC 
ACAAGGCAAA GAGCAATGAG TCTAGCCAGT ATTTTGACCA ACACCATGGA AGAACTTGAA 
GAATCCAGAC AGAAATGCCC ACCCTGTTGG TATAAATTTG CTAACATGTG TTTGATTTGG 
GACTGCTGTA AACCATGGTT AAAGGTGAAA CATCTCGTCA ACCTGGTTGT AATGGACCCG 
TTTGTTGACC TGGCCATCAC CATCTGCATT GTCCTAAATA CACTGTTTAT GGCCATGGAG 
CACTATCCTA TGACAGAGCA GTTCAGCAGT GTGCTGTCAG TTGGGAACCT GGTCTTCACT 
GGGATCTTCA CAGCAGAAAT GTTCCTCAAG ATAATAGCCA TGGATCCATA TTATTACTTT 
CAAGAGGGCT GGAATATTTT TGATGGTTTA ATCGTAAGCC TTAGTTTAAT GGAACTTGGT 
TTGGCAAACG TTGAAGGATT GTCAGTTCTC CGATCATTTC GACTGCTCCG AGTTTTCAAA 
TTGGCAAAAT CCTGGCCCAC CCTGAATATG CTGATAAAGA TCATCGGCAA CTCAGTGGGG 
GCTCTGGGCA ACCTCACCTT GGTGTTGGCC ATCATCGTCT TCATTTTTGC CGTGGTTGGC 
ATGCAGCTCT TTGGTAAGAG CTACAAAGAA TGTGTCTGCA AGATCTCCAA TGATTGTGAG 
CTCCCACGGT GGCACATGCA TGACTTCTTC CACTCCTTCC TGATTGTATT CCGTGTGTTG 
TGTGGAGAGT GGATAGAAAC CATGTGGGAC TGTATGGAGG TCGCTGGCCA AACCATGTGC 
CTTACTGTCT TCATGATGGT CATGGTGATT GGAAACCTGG TGGTTCTGAA TCTCTTTCTG 
GCCTTGCTCT TGAGTTCATT CAGTTCTGAC AACCTTGCAG CCACTGACGA TGATAATGAA 
ATGAACAATC TCCAGATTGC TGTGGGAAGG ATGCAGAAAG GAATCGATTA TGTTAAAAGA 
AAACTACGTG AATTTATTCA GAAAGCCTTT GTTAGAAAGC AGAAAGCCTT AGATGAAATT 
AAACCTCTTG AAGATCTAAA TAACAAAAAA GACAGCTGTA TTTCCAACCA TACAACTATA 
GAAATAGGAA AAGATCTCAA TTATCTCAAA GATGGAAATG GAACTACCAG TGGCATAGGC 
AGCAGTGTGG AAAAATATGT TGTGGATGAA AGTGATTACA TGTCATTTAT AAACAATCCC 
AGCCTCACTG TGACTGTACC AATTGCTGTC GGGGAATCTG ACTTTGAAAA TTTAAATACT 
GAAGAATTTA GCAGCGAGTC AGATATGGAG GAAAGCAAAG AGAAGCTAAA TGCAACTAGT 
TCATCTGAAG GCAGCACAGT TGATATAGGA GCTCCCGCTG AGGGAGAGCA ACCTGAGGTA 
GAACCCGAGG AATCTCTTGA ACCTGAAGCC TGTTTCACGG AAGACTGTGT ACGGAAGTTC 
AAGTGTTGTC AGATAAGCAT AGAAGAAGGC AAAGGGAAGC TCTGGTGGAA CTTGAGAAAA 
ACTTGCTATA AGATAGTGGA GCACAACTGG TTTGAAACTT TCATTGTCTT CATGATTCTT 
CTCAGCAGTG GGGCACTGGC CTTTGAAGAT ATATATATTG AGCAGCGAAA AACCATTAAG 
ACCATGTTAG AATATGCCGA CAAAGTTTTC ACTTACATAT TCATTCTGGA AATGCTTCTC 
AAGTGGGTCG CATATGGTTT TCAAATGTAT TTCACCAATG CCTGGTGCTG GCTGGATTTC 
CTGATTGTTG ACGTCTCATT GGTTAGCTTG ACTGCAAACG CCTTGGGTTA CTCAGAACTT 
GGCGCCATCA AATCCCTCAG GACACTCAGA GCTCTGAGGC CATTGAGAGC CCTATCCCGG 
TTTGAAGGAA TGAGGGTTGT TGTCAATGCT CTTCTAGGAG CCATTCCATC CATAATGAAT 
GTGCTCCTGG TTTGTCTTAT CTTTTGGCTA ATATTCAGCA TCATGGGAGT AAATCTTTTC 
GCTGGCAAGT TTTACCATTG TATTAACTAC ACCACTGGAG AAATGTTTGA TGTAAGTGTG 
GTCAACAACT ACAGCGAATG TAAAGCCCTC ATAGAGAGCA ATCAAACTGC CAGGTGGAAA 
AATGTGAAAG TAAACTTTGA TAATGTAGGA CTTGGATATC TTTCTCTGCT TCAAGTAGCC 
ACATTTAAGG GATGGATGGA CATCATGTAT GCAGCTGTTG ATTCACGAAA TGTAGAATTA 
CAACCCAAGT ATGAAGACAA TCTGTACATG TACCTTTATT TTGTCATCTT TATTATTTTT 
GGTTCATTCT TTACCCTGAA TCTTTTCATT GGTGTCATCA TAGATAACTT CAACCAACAG 
AAAAAGAAGT TTGGAGGTCA AGACATTTTT ATGACAGAAG AGCAGAAGAA ATACTACAAT 
GCAATGAAAA AACTGGGTTC AAAGAAACCA CAAAAACCCA TACCTCGACC TGCTAATAAA 
TTCCAAGGAA TGGTCTTTGA TTTTGTAACC AAACAAGTCT TTGATATCAG TATCATGATC 
CTCATCTGCC TCAACATGGT CACCATGATG GTGGAAACTG ATGACCAGAG TCAGGAGATG 
ACAAACATTC TGTACTGGAT TAATCTGGTG TTTATTGTTC TGTTCACTGG AGAATGTGTG 
CTGAAATTAA TCTCCCTTCG TTATTACTAT TTCACCATTG GATGGAATAT TTTTGATTTT 
GTGGTGGTCA TTCTCTCTAT CGTAGGCATG TTTCTGGCTG AGCTGATAGA AAAATACTTT 
GTGTCCCCCA CCCTGTTCCG AGTGATCCGG CTCGCCAGGA TCGGTCGAAT CTTGCGTCTG 
ATCAAAGGGG CGAAGGGGAT CCGCACACTG CTCTTTGCTC TGATGATGTC CCTCCCTGCC 
TTGTTTAACA TCGGCCTCCT GCTCTTCCTG GTCATGTTCA TCTACGCCAT CTTTGGAATG 
TCCAACTTTG CCTATGTCAA GAGGGAAGTT GGAATTGATG ACATGTTCAA CTTTGAGACC 
TTTGGCAACA GCATGATCTG CTTGTTCCAA ATTACCACCT CGGCTGGCTG GGATGGATTG 
CTAGCACCTA TTCTTAATAG TGGACCTCCA GACTGTGATC CTGAAAAAGA TCACCCTGGA 
AGCTCAGTTA AGGGAGACTG TGGGAACCCA TCTGTGGGGA TTTTCTTTTT TGTCAGTTAC 
ATCATCATCT CCTTTCTGGT TGTGGTGAAC ATGTACATCG CTGTCATCCT GGAGAACTTC 
AGTGTTGCCA CTGAGGAGAG TGCAGAGCCC CTGAGCGAGG ATGACTTTGA GATGTTCTAT 
GAAGTCTGGG AGAAGTTTGA TCCTGATGCC ACCCAGTTTA TAGAGTTCTC CAAGCTCTCT 
GATTTTGCAG CTGCCCTGGA TCCTCCTCTT CTCATAGCGA AACCAAACAA AGTCCAGCTC 
ATTGCCATGG ACCTGCCCAT GGTCAGTGGG GACCGGATCC ACTGCCTCGA CATTTTATTT 
GCCTTTACAA AGCGTGTTTT GGGTGAGAGT GGAGAGATGG ATGCCCTTAG AATACAGATG 
GAGGAGCGAT TCATGGCATC GAACCCCTCC AAAGTCTCCT ATGAACCCAT CACAACCACT 
CTGAAACGCA AACAAGAGGA GGTGTCTGCT ATCGTTATCC AGAGGGCTTA TAGACGCTAC 
CTCTTGAAGC AGAAAGTTAA AAAGGTTTCA TGTATCTATA AGAAAGACAA AGGCAAAGAA 
GGGGATGGAA CACCTATCAA AGAAGATATC CTCATTGACA AACTGAATGA GAATTCGACG 
CCAGAGAAAA CCGATGTGAC ACCTTCCACC ACTTCTCCAC CTTCGTATGA TAGTGTGACC 
AAACCAGAAA AAGAAAAATT TGAAAAAGAC AAATCAGAAA AGGAAGACAA AGGGAAAGAT 
ATCAGGGAAA GTAAAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022270448.1
CDS	162..6182
Translation	MAQSVLVPPGPDSFRFFTRESLAAIEQRIAEEKAKRPKQERKDEDDENGPKPNSDLEAGKSLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAISRFSATPALYILTPFNPIRKLAIKILVHSLFNVLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKILARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCLQWPPDNSSFEINITSFFNNSLDGNGTMFNRTVSMFNWDEYIEDKSHFYFLEGQNDALLCGNSSDAGQCPEGYICVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMLEQLKKQQEEAQAAAAAASAESREFSGAGGIGVFSESSSVASKLSSKSEKELKNRRKKKKQKEQSGEEEKEDGVRKSESEDSIRRKGFRFSLEGSRLTYEKRFSSPHQSLLSIRGSLFSPRRNSRASLFSFRGRTKDIGSENDFADDEHSTFEDNDSRRDSLFVPHRHGERRHSNVSQASRASRVIPILPVNGKMHSAVDCNGVVSLVGGPSALTSPAGQLLPEGTTTETELRKRRSSSYHVSMDLLEDPTTRQRAMSLASILTNTMEELEESRQKCPPCWYKFANMCLIWDCCKPWLKVKHLVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTEQFSSVLSVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGLIVSLSLMELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKISNDCELPRWHMHDFFHSFLIVFRVLCGEWIETMWDCMEVAGQTMCLTVFMMVMVIGNLVVLNLFLALLLSSFSSDNLAATDDDNEMNNLQIAVGRMQKGIDYVKRKLREFIQKAFVRKQKALDEIKPLEDLNNKKDSCISNHTTIEIGKDLNYLKDGNGTTSGIGSSVEKYVVDESDYMSFINNPSLTVTVPIAVGESDFENLNTEEFSSESDMEESKEKLNATSSSEGSTVDIGAPAEGEQPEVEPEESLEPEACFTEDCVRKFKCCQISIEEGKGKLWWNLRKTCYKIVEHNWFETFIVFMILLSSGALAFEDIYIEQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGFQMYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINYTTGEMFDVSVVNNYSECKALIESNQTARWKNVKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEDNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPANKFQGMVFDFVTKQVFDISIMILICLNMVTMMVETDDQSQEMTNILYWINLVFIVLFTGECVLKLISLRYYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSGPPDCDPEKDHPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFIEFSKLSDFAAALDPPLLIAKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYEPITTTLKRKQEEVSAIVIQRAYRRYLLKQKVKKVSCIYKKDKGKEGDGTPIKEDILIDKLNENSTPEKTDVTPSTTSPPSYDSVTKPEKEKFEKDKSEKEDKGKDIRESKK

Target ORF information:

RefSeq Version	XM_022414740.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris sodium channel protein type 2 subunit alpha (LOC478773), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022414740.1

ORF Insert Sequence:

ATGGCACAGT CAGTGCTGGT ACCACCAGGA CCTGACAGCT TCCGCTTCTT TACCAGGGAA 
TCCCTTGCTG CTATTGAACA ACGCATTGCA GAAGAGAAAG CTAAGAGACC CAAACAGGAG 
CGTAAGGATG AGGATGATGA AAATGGCCCA AAGCCAAACA GTGACTTGGA AGCAGGGAAA 
TCTCTTCCAT TTATTTATGG AGACATTCCT CCAGAGATGG TGTCAGAGCC CTTGGAGGAC 
CTGGACCCGT ACTATATCAA TAAGAAAACA TTTATAGTAT TGAATAAAGG GAAAGCAATC 
TCTCGATTCA GTGCCACCCC TGCCCTGTAC ATTTTAACTC CCTTCAACCC TATTAGAAAA 
TTAGCTATTA AGATTTTGGT ACATTCTTTA TTCAATGTGC TCATTATGTG CACTATTCTC 
ACCAACTGTG TATTTATGAC CATGAGTAAC CCTCCAGACT GGACAAAGAA TGTGGAGTAC 
ACCTTTACAG GAATTTATAC ATTTGAATCA CTTATAAAAA TACTTGCAAG GGGCTTTTGT 
TTAGAAGATT TCACATTTCT CCGGGATCCA TGGAATTGGT TGGATTTCAC AGTCATTACT 
TTTGCATACG TGACAGAGTT TGTGGATCTG GGCAATGTCT CAGCGCTGAG AACATTCAGA 
GTTCTCCGAG CACTGAAAAC AATTTCAGTC ATTCCAGGCC TGAAGACCAT TGTGGGGGCC 
CTGATCCAGT CGGTGAAGAA GCTCTCTGAT GTAATGATTC TGACTGTGTT CTGTCTGAGT 
GTGTTTGCAC TCATAGGACT GCAGCTGTTT ATGGGCAACC TGCGGAATAA ATGCTTGCAA 
TGGCCCCCAG ATAATTCTTC CTTTGAAATA AACATCACTT CCTTCTTTAA TAATTCATTG 
GATGGGAACG GTACCATGTT CAATAGGACA GTGAGCATGT TTAACTGGGA TGAATATATT 
GAGGATAAAA GTCACTTTTA TTTTTTGGAA GGGCAAAATG ATGCTCTGCT TTGTGGCAAC 
AGCTCAGATG CAGGCCAGTG TCCGGAAGGA TATATCTGTG TGAAGGCTGG TAGAAACCCC 
AACTATGGCT ATACAAGCTT TGATACCTTT AGTTGGGCCT TCTTGTCATT ATTTCGTCTC 
ATGACTCAAG ACTTCTGGGA AAACCTTTAC CAACTGACGC TACGTGCTGC TGGGAAAACA 
TATATGATAT TTTTTGTGCT GGTCATTTTC TTGGGCTCAT TCTACCTTAT AAATTTAATC 
CTGGCTGTGG TGGCTATGGC CTACGAGGAA CAGAATCAGG CAACTCTTGA GGAAGCTGAA 
CAGAAAGAAG CTGAATTTCA GCAGATGCTC GAACAACTAA AAAAACAACA AGAAGAAGCT 
CAGGCAGCAG CTGCAGCCGC ATCTGCAGAA TCAAGAGAGT TCAGTGGTGC TGGTGGGATA 
GGAGTTTTCT CAGAGAGTTC TTCAGTAGCA TCAAAGTTGA GCTCCAAGAG TGAAAAAGAG 
CTGAAAAACA GAAGGAAGAA AAAGAAGCAG AAAGAGCAGT CTGGAGAAGA AGAGAAGGAA 
GATGGGGTTC GCAAATCCGA ATCCGAAGAC AGTATAAGAA GAAAAGGTTT CCGTTTTTCC 
TTAGAAGGAA GCAGGCTGAC ATATGAAAAA AGGTTCTCTT CTCCACATCA GTCTTTATTG 
AGCATCCGTG GCTCCCTTTT CTCTCCAAGA CGCAACAGTA GGGCGAGCCT TTTCAGTTTC 
AGAGGCCGAA CAAAGGATAT TGGCTCTGAG AACGACTTTG CTGATGATGA GCACAGCACC 
TTTGAAGACA ATGACAGCCG GAGAGACTCT CTGTTTGTGC CACACAGACA TGGAGAGCGG 
CGTCATAGCA ATGTCAGCCA GGCCAGCCGG GCCTCCAGGG TGATCCCCAT CCTGCCGGTG 
AATGGAAAGA TGCACAGTGC AGTGGATTGC AACGGGGTCG TCTCGCTGGT TGGGGGCCCT 
TCTGCCCTCA CGTCTCCAGC TGGACAGCTC CTTCCGGAGG GCACGACTAC TGAAACAGAA 
CTAAGAAAGA GACGGTCCAG TTCTTACCAT GTTTCCATGG ATTTGCTGGA AGATCCTACC 
ACAAGGCAAA GAGCAATGAG TCTAGCCAGT ATTTTGACCA ACACCATGGA AGAACTTGAA 
GAATCCAGAC AGAAATGCCC ACCCTGTTGG TATAAATTTG CTAACATGTG TTTGATTTGG 
GACTGCTGTA AACCATGGTT AAAGGTGAAA CATCTCGTCA ACCTGGTTGT AATGGACCCG 
TTTGTTGACC TGGCCATCAC CATCTGCATT GTCCTAAATA CACTGTTTAT GGCCATGGAG 
CACTATCCTA TGACAGAGCA GTTCAGCAGT GTGCTGTCAG TTGGGAACCT GGTCTTCACT 
GGGATCTTCA CAGCAGAAAT GTTCCTCAAG ATAATAGCCA TGGATCCATA TTATTACTTT 
CAAGAGGGCT GGAATATTTT TGATGGTTTA ATCGTAAGCC TTAGTTTAAT GGAACTTGGT 
TTGGCAAACG TTGAAGGATT GTCAGTTCTC CGATCATTTC GACTGCTCCG AGTTTTCAAA 
TTGGCAAAAT CCTGGCCCAC CCTGAATATG CTGATAAAGA TCATCGGCAA CTCAGTGGGG 
GCTCTGGGCA ACCTCACCTT GGTGTTGGCC ATCATCGTCT TCATTTTTGC CGTGGTTGGC 
ATGCAGCTCT TTGGTAAGAG CTACAAAGAA TGTGTCTGCA AGATCTCCAA TGATTGTGAG 
CTCCCACGGT GGCACATGCA TGACTTCTTC CACTCCTTCC TGATTGTATT CCGTGTGTTG 
TGTGGAGAGT GGATAGAAAC CATGTGGGAC TGTATGGAGG TCGCTGGCCA AACCATGTGC 
CTTACTGTCT TCATGATGGT CATGGTGATT GGAAACCTGG TGGTTCTGAA TCTCTTTCTG 
GCCTTGCTCT TGAGTTCATT CAGTTCTGAC AACCTTGCAG CCACTGACGA TGATAATGAA 
ATGAACAATC TCCAGATTGC TGTGGGAAGG ATGCAGAAAG GAATCGATTA TGTTAAAAGA 
AAACTACGTG AATTTATTCA GAAAGCCTTT GTTAGAAAGC AGAAAGCCTT AGATGAAATT 
AAACCTCTTG AAGATCTAAA TAACAAAAAA GACAGCTGTA TTTCCAACCA TACAACTATA 
GAAATAGGAA AAGATCTCAA TTATCTCAAA GATGGAAATG GAACTACCAG TGGCATAGGC 
AGCAGTGTGG AAAAATATGT TGTGGATGAA AGTGATTACA TGTCATTTAT AAACAATCCC 
AGCCTCACTG TGACTGTACC AATTGCTGTC GGGGAATCTG ACTTTGAAAA TTTAAATACT 
GAAGAATTTA GCAGCGAGTC AGATATGGAG GAAAGCAAAG AGAAGCTAAA TGCAACTAGT 
TCATCTGAAG GCAGCACAGT TGATATAGGA GCTCCCGCTG AGGGAGAGCA ACCTGAGGTA 
GAACCCGAGG AATCTCTTGA ACCTGAAGCC TGTTTCACGG AAGACTGTGT ACGGAAGTTC 
AAGTGTTGTC AGATAAGCAT AGAAGAAGGC AAAGGGAAGC TCTGGTGGAA CTTGAGAAAA 
ACTTGCTATA AGATAGTGGA GCACAACTGG TTTGAAACTT TCATTGTCTT CATGATTCTT 
CTCAGCAGTG GGGCACTGGC CTTTGAAGAT ATATATATTG AGCAGCGAAA AACCATTAAG 
ACCATGTTAG AATATGCCGA CAAAGTTTTC ACTTACATAT TCATTCTGGA AATGCTTCTC 
AAGTGGGTCG CATATGGTTT TCAAATGTAT TTCACCAATG CCTGGTGCTG GCTGGATTTC 
CTGATTGTTG ACGTCTCATT GGTTAGCTTG ACTGCAAACG CCTTGGGTTA CTCAGAACTT 
GGCGCCATCA AATCCCTCAG GACACTCAGA GCTCTGAGGC CATTGAGAGC CCTATCCCGG 
TTTGAAGGAA TGAGGGTTGT TGTCAATGCT CTTCTAGGAG CCATTCCATC CATAATGAAT 
GTGCTCCTGG TTTGTCTTAT CTTTTGGCTA ATATTCAGCA TCATGGGAGT AAATCTTTTC 
GCTGGCAAGT TTTACCATTG TATTAACTAC ACCACTGGAG AAATGTTTGA TGTAAGTGTG 
GTCAACAACT ACAGCGAATG TAAAGCCCTC ATAGAGAGCA ATCAAACTGC CAGGTGGAAA 
AATGTGAAAG TAAACTTTGA TAATGTAGGA CTTGGATATC TTTCTCTGCT TCAAGTAGCC 
ACATTTAAGG GATGGATGGA CATCATGTAT GCAGCTGTTG ATTCACGAAA TGTAGAATTA 
CAACCCAAGT ATGAAGACAA TCTGTACATG TACCTTTATT TTGTCATCTT TATTATTTTT 
GGTTCATTCT TTACCCTGAA TCTTTTCATT GGTGTCATCA TAGATAACTT CAACCAACAG 
AAAAAGAAGT TTGGAGGTCA AGACATTTTT ATGACAGAAG AGCAGAAGAA ATACTACAAT 
GCAATGAAAA AACTGGGTTC AAAGAAACCA CAAAAACCCA TACCTCGACC TGCTAATAAA 
TTCCAAGGAA TGGTCTTTGA TTTTGTAACC AAACAAGTCT TTGATATCAG TATCATGATC 
CTCATCTGCC TCAACATGGT CACCATGATG GTGGAAACTG ATGACCAGAG TCAGGAGATG 
ACAAACATTC TGTACTGGAT TAATCTGGTG TTTATTGTTC TGTTCACTGG AGAATGTGTG 
CTGAAATTAA TCTCCCTTCG TTATTACTAT TTCACCATTG GATGGAATAT TTTTGATTTT 
GTGGTGGTCA TTCTCTCTAT CGTAGGCATG TTTCTGGCTG AGCTGATAGA AAAATACTTT 
GTGTCCCCCA CCCTGTTCCG AGTGATCCGG CTCGCCAGGA TCGGTCGAAT CTTGCGTCTG 
ATCAAAGGGG CGAAGGGGAT CCGCACACTG CTCTTTGCTC TGATGATGTC CCTCCCTGCC 
TTGTTTAACA TCGGCCTCCT GCTCTTCCTG GTCATGTTCA TCTACGCCAT CTTTGGAATG 
TCCAACTTTG CCTATGTCAA GAGGGAAGTT GGAATTGATG ACATGTTCAA CTTTGAGACC 
TTTGGCAACA GCATGATCTG CTTGTTCCAA ATTACCACCT CGGCTGGCTG GGATGGATTG 
CTAGCACCTA TTCTTAATAG TGGACCTCCA GACTGTGATC CTGAAAAAGA TCACCCTGGA 
AGCTCAGTTA AGGGAGACTG TGGGAACCCA TCTGTGGGGA TTTTCTTTTT TGTCAGTTAC 
ATCATCATCT CCTTTCTGGT TGTGGTGAAC ATGTACATCG CTGTCATCCT GGAGAACTTC 
AGTGTTGCCA CTGAGGAGAG TGCAGAGCCC CTGAGCGAGG ATGACTTTGA GATGTTCTAT 
GAAGTCTGGG AGAAGTTTGA TCCTGATGCC ACCCAGTTTA TAGAGTTCTC CAAGCTCTCT 
GATTTTGCAG CTGCCCTGGA TCCTCCTCTT CTCATAGCGA AACCAAACAA AGTCCAGCTC 
ATTGCCATGG ACCTGCCCAT GGTCAGTGGG GACCGGATCC ACTGCCTCGA CATTTTATTT 
GCCTTTACAA AGCGTGTTTT GGGTGAGAGT GGAGAGATGG ATGCCCTTAG AATACAGATG 
GAGGAGCGAT TCATGGCATC GAACCCCTCC AAAGTCTCCT ATGAACCCAT CACAACCACT 
CTGAAACGCA AACAAGAGGA GGTGTCTGCT ATCGTTATCC AGAGGGCTTA TAGACGCTAC 
CTCTTGAAGC AGAAAGTTAA AAAGGTTTCA TGTATCTATA AGAAAGACAA AGGCAAAGAA 
GGGGATGGAA CACCTATCAA AGAAGATATC CTCATTGACA AACTGAATGA GAATTCGACG 
CCAGAGAAAA CCGATGTGAC ACCTTCCACC ACTTCTCCAC CTTCGTATGA TAGTGTGACC 
AAACCAGAAA AAGAAAAATT TGAAAAAGAC AAATCAGAAA AGGAAGACAA AGGGAAAGAT 
ATCAGGGAAA GTAAAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC478773 ( XM_014110825.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013966300.1 Canis lupus familiaris sodium channel protein type 2 subunit alpha (LOC478773), transcript variant X3, mRNA.

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CloneID	OCe31042
Clone ID Related Accession (Same CDS sequence)	XM_535939.5 , XM_014110824.2 , XM_022414740.1 , XM_014110825.2 , XM_014110824.1 , XM_014110825.1 , XM_535939.6
Accession Version	XM_014110825.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	sodium channel protein type 2 subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006618.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014110825.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGT CAGTGCTGGT ACCACCAGGA CCTGACAGCT TCCGCTTCTT TACCAGGGAA 
TCCCTTGCTG CTATTGAACA ACGCATTGCA GAAGAGAAAG CTAAGAGACC CAAACAGGAG 
CGTAAGGATG AGGATGATGA AAATGGCCCA AAGCCAAACA GTGACTTGGA AGCAGGGAAA 
TCTCTTCCAT TTATTTATGG AGACATTCCT CCAGAGATGG TGTCAGAGCC CTTGGAGGAC 
CTGGACCCGT ACTATATCAA TAAGAAAACA TTTATAGTAT TGAATAAAGG GAAAGCAATC 
TCTCGATTCA GTGCCACCCC TGCCCTGTAC ATTTTAACTC CCTTCAACCC TATTAGAAAA 
TTAGCTATTA AGATTTTGGT ACATTCTTTA TTCAATGTGC TCATTATGTG CACTATTCTC 
ACCAACTGTG TATTTATGAC CATGAGTAAC CCTCCAGACT GGACAAAGAA TGTGGAGTAC 
ACCTTTACAG GAATTTATAC ATTTGAATCA CTTATAAAAA TACTTGCAAG GGGCTTTTGT 
TTAGAAGATT TCACATTTCT CCGGGATCCA TGGAATTGGT TGGATTTCAC AGTCATTACT 
TTTGCATACG TGACAGAGTT TGTGGATCTG GGCAATGTCT CAGCGCTGAG AACATTCAGA 
GTTCTCCGAG CACTGAAAAC AATTTCAGTC ATTCCAGGCC TGAAGACCAT TGTGGGGGCC 
CTGATCCAGT CGGTGAAGAA GCTCTCTGAT GTAATGATTC TGACTGTGTT CTGTCTGAGT 
GTGTTTGCAC TCATAGGACT GCAGCTGTTT ATGGGCAACC TGCGGAATAA ATGCTTGCAA 
TGGCCCCCAG ATAATTCTTC CTTTGAAATA AACATCACTT CCTTCTTTAA TAATTCATTG 
GATGGGAACG GTACCATGTT CAATAGGACA GTGAGCATGT TTAACTGGGA TGAATATATT 
GAGGATAAAA GTCACTTTTA TTTTTTGGAA GGGCAAAATG ATGCTCTGCT TTGTGGCAAC 
AGCTCAGATG CAGGCCAGTG TCCGGAAGGA TATATCTGTG TGAAGGCTGG TAGAAACCCC 
AACTATGGCT ATACAAGCTT TGATACCTTT AGTTGGGCCT TCTTGTCATT ATTTCGTCTC 
ATGACTCAAG ACTTCTGGGA AAACCTTTAC CAACTGACGC TACGTGCTGC TGGGAAAACA 
TATATGATAT TTTTTGTGCT GGTCATTTTC TTGGGCTCAT TCTACCTTAT AAATTTAATC 
CTGGCTGTGG TGGCTATGGC CTACGAGGAA CAGAATCAGG CAACTCTTGA GGAAGCTGAA 
CAGAAAGAAG CTGAATTTCA GCAGATGCTC GAACAACTAA AAAAACAACA AGAAGAAGCT 
CAGGCAGCAG CTGCAGCCGC ATCTGCAGAA TCAAGAGAGT TCAGTGGTGC TGGTGGGATA 
GGAGTTTTCT CAGAGAGTTC TTCAGTAGCA TCAAAGTTGA GCTCCAAGAG TGAAAAAGAG 
CTGAAAAACA GAAGGAAGAA AAAGAAGCAG AAAGAGCAGT CTGGAGAAGA AGAGAAGGAA 
GATGGGGTTC GCAAATCCGA ATCCGAAGAC AGTATAAGAA GAAAAGGTTT CCGTTTTTCC 
TTAGAAGGAA GCAGGCTGAC ATATGAAAAA AGGTTCTCTT CTCCACATCA GTCTTTATTG 
AGCATCCGTG GCTCCCTTTT CTCTCCAAGA CGCAACAGTA GGGCGAGCCT TTTCAGTTTC 
AGAGGCCGAA CAAAGGATAT TGGCTCTGAG AACGACTTTG CTGATGATGA GCACAGCACC 
TTTGAAGACA ATGACAGCCG GAGAGACTCT CTGTTTGTGC CACACAGACA TGGAGAGCGG 
CGTCATAGCA ATGTCAGCCA GGCCAGCCGG GCCTCCAGGG TGATCCCCAT CCTGCCGGTG 
AATGGAAAGA TGCACAGTGC AGTGGATTGC AACGGGGTCG TCTCGCTGGT TGGGGGCCCT 
TCTGCCCTCA CGTCTCCAGC TGGACAGCTC CTTCCGGAGG GCACGACTAC TGAAACAGAA 
CTAAGAAAGA GACGGTCCAG TTCTTACCAT GTTTCCATGG ATTTGCTGGA AGATCCTACC 
ACAAGGCAAA GAGCAATGAG TCTAGCCAGT ATTTTGACCA ACACCATGGA AGAACTTGAA 
GAATCCAGAC AGAAATGCCC ACCCTGTTGG TATAAATTTG CTAACATGTG TTTGATTTGG 
GACTGCTGTA AACCATGGTT AAAGGTGAAA CATCTCGTCA ACCTGGTTGT AATGGACCCG 
TTTGTTGACC TGGCCATCAC CATCTGCATT GTCCTAAATA CACTGTTTAT GGCCATGGAG 
CACTATCCTA TGACAGAGCA GTTCAGCAGT GTGCTGTCAG TTGGGAACCT GGTCTTCACT 
GGGATCTTCA CAGCAGAAAT GTTCCTCAAG ATAATAGCCA TGGATCCATA TTATTACTTT 
CAAGAGGGCT GGAATATTTT TGATGGTTTA ATCGTAAGCC TTAGTTTAAT GGAACTTGGT 
TTGGCAAACG TTGAAGGATT GTCAGTTCTC CGATCATTTC GACTGCTCCG AGTTTTCAAA 
TTGGCAAAAT CCTGGCCCAC CCTGAATATG CTGATAAAGA TCATCGGCAA CTCAGTGGGG 
GCTCTGGGCA ACCTCACCTT GGTGTTGGCC ATCATCGTCT TCATTTTTGC CGTGGTTGGC 
ATGCAGCTCT TTGGTAAGAG CTACAAAGAA TGTGTCTGCA AGATCTCCAA TGATTGTGAG 
CTCCCACGGT GGCACATGCA TGACTTCTTC CACTCCTTCC TGATTGTATT CCGTGTGTTG 
TGTGGAGAGT GGATAGAAAC CATGTGGGAC TGTATGGAGG TCGCTGGCCA AACCATGTGC 
CTTACTGTCT TCATGATGGT CATGGTGATT GGAAACCTGG TGGTTCTGAA TCTCTTTCTG 
GCCTTGCTCT TGAGTTCATT CAGTTCTGAC AACCTTGCAG CCACTGACGA TGATAATGAA 
ATGAACAATC TCCAGATTGC TGTGGGAAGG ATGCAGAAAG GAATCGATTA TGTTAAAAGA 
AAACTACGTG AATTTATTCA GAAAGCCTTT GTTAGAAAGC AGAAAGCCTT AGATGAAATT 
AAACCTCTTG AAGATCTAAA TAACAAAAAA GACAGCTGTA TTTCCAACCA TACAACTATA 
GAAATAGGAA AAGATCTCAA TTATCTCAAA GATGGAAATG GAACTACCAG TGGCATAGGC 
AGCAGTGTGG AAAAATATGT TGTGGATGAA AGTGATTACA TGTCATTTAT AAACAATCCC 
AGCCTCACTG TGACTGTACC AATTGCTGTC GGGGAATCTG ACTTTGAAAA TTTAAATACT 
GAAGAATTTA GCAGCGAGTC AGATATGGAG GAAAGCAAAG AGAAGCTAAA TGCAACTAGT 
TCATCTGAAG GCAGCACAGT TGATATAGGA GCTCCCGCTG AGGGAGAGCA ACCTGAGGTA 
GAACCCGAGG AATCTCTTGA ACCTGAAGCC TGTTTCACGG AAGACTGTGT ACGGAAGTTC 
AAGTGTTGTC AGATAAGCAT AGAAGAAGGC AAAGGGAAGC TCTGGTGGAA CTTGAGAAAA 
ACTTGCTATA AGATAGTGGA GCACAACTGG TTTGAAACTT TCATTGTCTT CATGATTCTT 
CTCAGCAGTG GGGCACTGGC CTTTGAAGAT ATATATATTG AGCAGCGAAA AACCATTAAG 
ACCATGTTAG AATATGCCGA CAAAGTTTTC ACTTACATAT TCATTCTGGA AATGCTTCTC 
AAGTGGGTCG CATATGGTTT TCAAATGTAT TTCACCAATG CCTGGTGCTG GCTGGATTTC 
CTGATTGTTG ACGTCTCATT GGTTAGCTTG ACTGCAAACG CCTTGGGTTA CTCAGAACTT 
GGCGCCATCA AATCCCTCAG GACACTCAGA GCTCTGAGGC CATTGAGAGC CCTATCCCGG 
TTTGAAGGAA TGAGGGTTGT TGTCAATGCT CTTCTAGGAG CCATTCCATC CATAATGAAT 
GTGCTCCTGG TTTGTCTTAT CTTTTGGCTA ATATTCAGCA TCATGGGAGT AAATCTTTTC 
GCTGGCAAGT TTTACCATTG TATTAACTAC ACCACTGGAG AAATGTTTGA TGTAAGTGTG 
GTCAACAACT ACAGCGAATG TAAAGCCCTC ATAGAGAGCA ATCAAACTGC CAGGTGGAAA 
AATGTGAAAG TAAACTTTGA TAATGTAGGA CTTGGATATC TTTCTCTGCT TCAAGTAGCC 
ACATTTAAGG GATGGATGGA CATCATGTAT GCAGCTGTTG ATTCACGAAA TGTAGAATTA 
CAACCCAAGT ATGAAGACAA TCTGTACATG TACCTTTATT TTGTCATCTT TATTATTTTT 
GGTTCATTCT TTACCCTGAA TCTTTTCATT GGTGTCATCA TAGATAACTT CAACCAACAG 
AAAAAGAAGT TTGGAGGTCA AGACATTTTT ATGACAGAAG AGCAGAAGAA ATACTACAAT 
GCAATGAAAA AACTGGGTTC AAAGAAACCA CAAAAACCCA TACCTCGACC TGCTAATAAA 
TTCCAAGGAA TGGTCTTTGA TTTTGTAACC AAACAAGTCT TTGATATCAG TATCATGATC 
CTCATCTGCC TCAACATGGT CACCATGATG GTGGAAACTG ATGACCAGAG TCAGGAGATG 
ACAAACATTC TGTACTGGAT TAATCTGGTG TTTATTGTTC TGTTCACTGG AGAATGTGTG 
CTGAAATTAA TCTCCCTTCG TTATTACTAT TTCACCATTG GATGGAATAT TTTTGATTTT 
GTGGTGGTCA TTCTCTCTAT CGTAGGCATG TTTCTGGCTG AGCTGATAGA AAAATACTTT 
GTGTCCCCCA CCCTGTTCCG AGTGATCCGG CTCGCCAGGA TCGGTCGAAT CTTGCGTCTG 
ATCAAAGGGG CGAAGGGGAT CCGCACACTG CTCTTTGCTC TGATGATGTC CCTCCCTGCC 
TTGTTTAACA TCGGCCTCCT GCTCTTCCTG GTCATGTTCA TCTACGCCAT CTTTGGAATG 
TCCAACTTTG CCTATGTCAA GAGGGAAGTT GGAATTGATG ACATGTTCAA CTTTGAGACC 
TTTGGCAACA GCATGATCTG CTTGTTCCAA ATTACCACCT CGGCTGGCTG GGATGGATTG 
CTAGCACCTA TTCTTAATAG TGGACCTCCA GACTGTGATC CTGAAAAAGA TCACCCTGGA 
AGCTCAGTTA AGGGAGACTG TGGGAACCCA TCTGTGGGGA TTTTCTTTTT TGTCAGTTAC 
ATCATCATCT CCTTTCTGGT TGTGGTGAAC ATGTACATCG CTGTCATCCT GGAGAACTTC 
AGTGTTGCCA CTGAGGAGAG TGCAGAGCCC CTGAGCGAGG ATGACTTTGA GATGTTCTAT 
GAAGTCTGGG AGAAGTTTGA TCCTGATGCC ACCCAGTTTA TAGAGTTCTC CAAGCTCTCT 
GATTTTGCAG CTGCCCTGGA TCCTCCTCTT CTCATAGCGA AACCAAACAA AGTCCAGCTC 
ATTGCCATGG ACCTGCCCAT GGTCAGTGGG GACCGGATCC ACTGCCTCGA CATTTTATTT 
GCCTTTACAA AGCGTGTTTT GGGTGAGAGT GGAGAGATGG ATGCCCTTAG AATACAGATG 
GAGGAGCGAT TCATGGCATC GAACCCCTCC AAAGTCTCCT ATGAACCCAT CACAACCACT 
CTGAAACGCA AACAAGAGGA GGTGTCTGCT ATCGTTATCC AGAGGGCTTA TAGACGCTAC 
CTCTTGAAGC AGAAAGTTAA AAAGGTTTCA TGTATCTATA AGAAAGACAA AGGCAAAGAA 
GGGGATGGAA CACCTATCAA AGAAGATATC CTCATTGACA AACTGAATGA GAATTCGACG 
CCAGAGAAAA CCGATGTGAC ACCTTCCACC ACTTCTCCAC CTTCGTATGA TAGTGTGACC 
AAACCAGAAA AAGAAAAATT TGAAAAAGAC AAATCAGAAA AGGAAGACAA AGGGAAAGAT 
ATCAGGGAAA GTAAAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013966300.1
CDS	159..6179
Translation	MAQSVLVPPGPDSFRFFTRESLAAIEQRIAEEKAKRPKQERKDEDDENGPKPNSDLEAGKSLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAISRFSATPALYILTPFNPIRKLAIKILVHSLFNVLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKILARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCLQWPPDNSSFEINITSFFNNSLDGNGTMFNRTVSMFNWDEYIEDKSHFYFLEGQNDALLCGNSSDAGQCPEGYICVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMLEQLKKQQEEAQAAAAAASAESREFSGAGGIGVFSESSSVASKLSSKSEKELKNRRKKKKQKEQSGEEEKEDGVRKSESEDSIRRKGFRFSLEGSRLTYEKRFSSPHQSLLSIRGSLFSPRRNSRASLFSFRGRTKDIGSENDFADDEHSTFEDNDSRRDSLFVPHRHGERRHSNVSQASRASRVIPILPVNGKMHSAVDCNGVVSLVGGPSALTSPAGQLLPEGTTTETELRKRRSSSYHVSMDLLEDPTTRQRAMSLASILTNTMEELEESRQKCPPCWYKFANMCLIWDCCKPWLKVKHLVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTEQFSSVLSVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGLIVSLSLMELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKISNDCELPRWHMHDFFHSFLIVFRVLCGEWIETMWDCMEVAGQTMCLTVFMMVMVIGNLVVLNLFLALLLSSFSSDNLAATDDDNEMNNLQIAVGRMQKGIDYVKRKLREFIQKAFVRKQKALDEIKPLEDLNNKKDSCISNHTTIEIGKDLNYLKDGNGTTSGIGSSVEKYVVDESDYMSFINNPSLTVTVPIAVGESDFENLNTEEFSSESDMEESKEKLNATSSSEGSTVDIGAPAEGEQPEVEPEESLEPEACFTEDCVRKFKCCQISIEEGKGKLWWNLRKTCYKIVEHNWFETFIVFMILLSSGALAFEDIYIEQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGFQMYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINYTTGEMFDVSVVNNYSECKALIESNQTARWKNVKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEDNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPANKFQGMVFDFVTKQVFDISIMILICLNMVTMMVETDDQSQEMTNILYWINLVFIVLFTGECVLKLISLRYYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSGPPDCDPEKDHPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFIEFSKLSDFAAALDPPLLIAKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYEPITTTLKRKQEEVSAIVIQRAYRRYLLKQKVKKVSCIYKKDKGKEGDGTPIKEDILIDKLNENSTPEKTDVTPSTTSPPSYDSVTKPEKEKFEKDKSEKEDKGKDIRESKK

Target ORF information:

RefSeq Version	XM_014110825.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris sodium channel protein type 2 subunit alpha (LOC478773), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014110825.2

ORF Insert Sequence:

ATGGCACAGT CAGTGCTGGT ACCACCAGGA CCTGACAGCT TCCGCTTCTT TACCAGGGAA 
TCCCTTGCTG CTATTGAACA ACGCATTGCA GAAGAGAAAG CTAAGAGACC CAAACAGGAG 
CGTAAGGATG AGGATGATGA AAATGGCCCA AAGCCAAACA GTGACTTGGA AGCAGGGAAA 
TCTCTTCCAT TTATTTATGG AGACATTCCT CCAGAGATGG TGTCAGAGCC CTTGGAGGAC 
CTGGACCCGT ACTATATCAA TAAGAAAACA TTTATAGTAT TGAATAAAGG GAAAGCAATC 
TCTCGATTCA GTGCCACCCC TGCCCTGTAC ATTTTAACTC CCTTCAACCC TATTAGAAAA 
TTAGCTATTA AGATTTTGGT ACATTCTTTA TTCAATGTGC TCATTATGTG CACTATTCTC 
ACCAACTGTG TATTTATGAC CATGAGTAAC CCTCCAGACT GGACAAAGAA TGTGGAGTAC 
ACCTTTACAG GAATTTATAC ATTTGAATCA CTTATAAAAA TACTTGCAAG GGGCTTTTGT 
TTAGAAGATT TCACATTTCT CCGGGATCCA TGGAATTGGT TGGATTTCAC AGTCATTACT 
TTTGCATACG TGACAGAGTT TGTGGATCTG GGCAATGTCT CAGCGCTGAG AACATTCAGA 
GTTCTCCGAG CACTGAAAAC AATTTCAGTC ATTCCAGGCC TGAAGACCAT TGTGGGGGCC 
CTGATCCAGT CGGTGAAGAA GCTCTCTGAT GTAATGATTC TGACTGTGTT CTGTCTGAGT 
GTGTTTGCAC TCATAGGACT GCAGCTGTTT ATGGGCAACC TGCGGAATAA ATGCTTGCAA 
TGGCCCCCAG ATAATTCTTC CTTTGAAATA AACATCACTT CCTTCTTTAA TAATTCATTG 
GATGGGAACG GTACCATGTT CAATAGGACA GTGAGCATGT TTAACTGGGA TGAATATATT 
GAGGATAAAA GTCACTTTTA TTTTTTGGAA GGGCAAAATG ATGCTCTGCT TTGTGGCAAC 
AGCTCAGATG CAGGCCAGTG TCCGGAAGGA TATATCTGTG TGAAGGCTGG TAGAAACCCC 
AACTATGGCT ATACAAGCTT TGATACCTTT AGTTGGGCCT TCTTGTCATT ATTTCGTCTC 
ATGACTCAAG ACTTCTGGGA AAACCTTTAC CAACTGACGC TACGTGCTGC TGGGAAAACA 
TATATGATAT TTTTTGTGCT GGTCATTTTC TTGGGCTCAT TCTACCTTAT AAATTTAATC 
CTGGCTGTGG TGGCTATGGC CTACGAGGAA CAGAATCAGG CAACTCTTGA GGAAGCTGAA 
CAGAAAGAAG CTGAATTTCA GCAGATGCTC GAACAACTAA AAAAACAACA AGAAGAAGCT 
CAGGCAGCAG CTGCAGCCGC ATCTGCAGAA TCAAGAGAGT TCAGTGGTGC TGGTGGGATA 
GGAGTTTTCT CAGAGAGTTC TTCAGTAGCA TCAAAGTTGA GCTCCAAGAG TGAAAAAGAG 
CTGAAAAACA GAAGGAAGAA AAAGAAGCAG AAAGAGCAGT CTGGAGAAGA AGAGAAGGAA 
GATGGGGTTC GCAAATCCGA ATCCGAAGAC AGTATAAGAA GAAAAGGTTT CCGTTTTTCC 
TTAGAAGGAA GCAGGCTGAC ATATGAAAAA AGGTTCTCTT CTCCACATCA GTCTTTATTG 
AGCATCCGTG GCTCCCTTTT CTCTCCAAGA CGCAACAGTA GGGCGAGCCT TTTCAGTTTC 
AGAGGCCGAA CAAAGGATAT TGGCTCTGAG AACGACTTTG CTGATGATGA GCACAGCACC 
TTTGAAGACA ATGACAGCCG GAGAGACTCT CTGTTTGTGC CACACAGACA TGGAGAGCGG 
CGTCATAGCA ATGTCAGCCA GGCCAGCCGG GCCTCCAGGG TGATCCCCAT CCTGCCGGTG 
AATGGAAAGA TGCACAGTGC AGTGGATTGC AACGGGGTCG TCTCGCTGGT TGGGGGCCCT 
TCTGCCCTCA CGTCTCCAGC TGGACAGCTC CTTCCGGAGG GCACGACTAC TGAAACAGAA 
CTAAGAAAGA GACGGTCCAG TTCTTACCAT GTTTCCATGG ATTTGCTGGA AGATCCTACC 
ACAAGGCAAA GAGCAATGAG TCTAGCCAGT ATTTTGACCA ACACCATGGA AGAACTTGAA 
GAATCCAGAC AGAAATGCCC ACCCTGTTGG TATAAATTTG CTAACATGTG TTTGATTTGG 
GACTGCTGTA AACCATGGTT AAAGGTGAAA CATCTCGTCA ACCTGGTTGT AATGGACCCG 
TTTGTTGACC TGGCCATCAC CATCTGCATT GTCCTAAATA CACTGTTTAT GGCCATGGAG 
CACTATCCTA TGACAGAGCA GTTCAGCAGT GTGCTGTCAG TTGGGAACCT GGTCTTCACT 
GGGATCTTCA CAGCAGAAAT GTTCCTCAAG ATAATAGCCA TGGATCCATA TTATTACTTT 
CAAGAGGGCT GGAATATTTT TGATGGTTTA ATCGTAAGCC TTAGTTTAAT GGAACTTGGT 
TTGGCAAACG TTGAAGGATT GTCAGTTCTC CGATCATTTC GACTGCTCCG AGTTTTCAAA 
TTGGCAAAAT CCTGGCCCAC CCTGAATATG CTGATAAAGA TCATCGGCAA CTCAGTGGGG 
GCTCTGGGCA ACCTCACCTT GGTGTTGGCC ATCATCGTCT TCATTTTTGC CGTGGTTGGC 
ATGCAGCTCT TTGGTAAGAG CTACAAAGAA TGTGTCTGCA AGATCTCCAA TGATTGTGAG 
CTCCCACGGT GGCACATGCA TGACTTCTTC CACTCCTTCC TGATTGTATT CCGTGTGTTG 
TGTGGAGAGT GGATAGAAAC CATGTGGGAC TGTATGGAGG TCGCTGGCCA AACCATGTGC 
CTTACTGTCT TCATGATGGT CATGGTGATT GGAAACCTGG TGGTTCTGAA TCTCTTTCTG 
GCCTTGCTCT TGAGTTCATT CAGTTCTGAC AACCTTGCAG CCACTGACGA TGATAATGAA 
ATGAACAATC TCCAGATTGC TGTGGGAAGG ATGCAGAAAG GAATCGATTA TGTTAAAAGA 
AAACTACGTG AATTTATTCA GAAAGCCTTT GTTAGAAAGC AGAAAGCCTT AGATGAAATT 
AAACCTCTTG AAGATCTAAA TAACAAAAAA GACAGCTGTA TTTCCAACCA TACAACTATA 
GAAATAGGAA AAGATCTCAA TTATCTCAAA GATGGAAATG GAACTACCAG TGGCATAGGC 
AGCAGTGTGG AAAAATATGT TGTGGATGAA AGTGATTACA TGTCATTTAT AAACAATCCC 
AGCCTCACTG TGACTGTACC AATTGCTGTC GGGGAATCTG ACTTTGAAAA TTTAAATACT 
GAAGAATTTA GCAGCGAGTC AGATATGGAG GAAAGCAAAG AGAAGCTAAA TGCAACTAGT 
TCATCTGAAG GCAGCACAGT TGATATAGGA GCTCCCGCTG AGGGAGAGCA ACCTGAGGTA 
GAACCCGAGG AATCTCTTGA ACCTGAAGCC TGTTTCACGG AAGACTGTGT ACGGAAGTTC 
AAGTGTTGTC AGATAAGCAT AGAAGAAGGC AAAGGGAAGC TCTGGTGGAA CTTGAGAAAA 
ACTTGCTATA AGATAGTGGA GCACAACTGG TTTGAAACTT TCATTGTCTT CATGATTCTT 
CTCAGCAGTG GGGCACTGGC CTTTGAAGAT ATATATATTG AGCAGCGAAA AACCATTAAG 
ACCATGTTAG AATATGCCGA CAAAGTTTTC ACTTACATAT TCATTCTGGA AATGCTTCTC 
AAGTGGGTCG CATATGGTTT TCAAATGTAT TTCACCAATG CCTGGTGCTG GCTGGATTTC 
CTGATTGTTG ACGTCTCATT GGTTAGCTTG ACTGCAAACG CCTTGGGTTA CTCAGAACTT 
GGCGCCATCA AATCCCTCAG GACACTCAGA GCTCTGAGGC CATTGAGAGC CCTATCCCGG 
TTTGAAGGAA TGAGGGTTGT TGTCAATGCT CTTCTAGGAG CCATTCCATC CATAATGAAT 
GTGCTCCTGG TTTGTCTTAT CTTTTGGCTA ATATTCAGCA TCATGGGAGT AAATCTTTTC 
GCTGGCAAGT TTTACCATTG TATTAACTAC ACCACTGGAG AAATGTTTGA TGTAAGTGTG 
GTCAACAACT ACAGCGAATG TAAAGCCCTC ATAGAGAGCA ATCAAACTGC CAGGTGGAAA 
AATGTGAAAG TAAACTTTGA TAATGTAGGA CTTGGATATC TTTCTCTGCT TCAAGTAGCC 
ACATTTAAGG GATGGATGGA CATCATGTAT GCAGCTGTTG ATTCACGAAA TGTAGAATTA 
CAACCCAAGT ATGAAGACAA TCTGTACATG TACCTTTATT TTGTCATCTT TATTATTTTT 
GGTTCATTCT TTACCCTGAA TCTTTTCATT GGTGTCATCA TAGATAACTT CAACCAACAG 
AAAAAGAAGT TTGGAGGTCA AGACATTTTT ATGACAGAAG AGCAGAAGAA ATACTACAAT 
GCAATGAAAA AACTGGGTTC AAAGAAACCA CAAAAACCCA TACCTCGACC TGCTAATAAA 
TTCCAAGGAA TGGTCTTTGA TTTTGTAACC AAACAAGTCT TTGATATCAG TATCATGATC 
CTCATCTGCC TCAACATGGT CACCATGATG GTGGAAACTG ATGACCAGAG TCAGGAGATG 
ACAAACATTC TGTACTGGAT TAATCTGGTG TTTATTGTTC TGTTCACTGG AGAATGTGTG 
CTGAAATTAA TCTCCCTTCG TTATTACTAT TTCACCATTG GATGGAATAT TTTTGATTTT 
GTGGTGGTCA TTCTCTCTAT CGTAGGCATG TTTCTGGCTG AGCTGATAGA AAAATACTTT 
GTGTCCCCCA CCCTGTTCCG AGTGATCCGG CTCGCCAGGA TCGGTCGAAT CTTGCGTCTG 
ATCAAAGGGG CGAAGGGGAT CCGCACACTG CTCTTTGCTC TGATGATGTC CCTCCCTGCC 
TTGTTTAACA TCGGCCTCCT GCTCTTCCTG GTCATGTTCA TCTACGCCAT CTTTGGAATG 
TCCAACTTTG CCTATGTCAA GAGGGAAGTT GGAATTGATG ACATGTTCAA CTTTGAGACC 
TTTGGCAACA GCATGATCTG CTTGTTCCAA ATTACCACCT CGGCTGGCTG GGATGGATTG 
CTAGCACCTA TTCTTAATAG TGGACCTCCA GACTGTGATC CTGAAAAAGA TCACCCTGGA 
AGCTCAGTTA AGGGAGACTG TGGGAACCCA TCTGTGGGGA TTTTCTTTTT TGTCAGTTAC 
ATCATCATCT CCTTTCTGGT TGTGGTGAAC ATGTACATCG CTGTCATCCT GGAGAACTTC 
AGTGTTGCCA CTGAGGAGAG TGCAGAGCCC CTGAGCGAGG ATGACTTTGA GATGTTCTAT 
GAAGTCTGGG AGAAGTTTGA TCCTGATGCC ACCCAGTTTA TAGAGTTCTC CAAGCTCTCT 
GATTTTGCAG CTGCCCTGGA TCCTCCTCTT CTCATAGCGA AACCAAACAA AGTCCAGCTC 
ATTGCCATGG ACCTGCCCAT GGTCAGTGGG GACCGGATCC ACTGCCTCGA CATTTTATTT 
GCCTTTACAA AGCGTGTTTT GGGTGAGAGT GGAGAGATGG ATGCCCTTAG AATACAGATG 
GAGGAGCGAT TCATGGCATC GAACCCCTCC AAAGTCTCCT ATGAACCCAT CACAACCACT 
CTGAAACGCA AACAAGAGGA GGTGTCTGCT ATCGTTATCC AGAGGGCTTA TAGACGCTAC 
CTCTTGAAGC AGAAAGTTAA AAAGGTTTCA TGTATCTATA AGAAAGACAA AGGCAAAGAA 
GGGGATGGAA CACCTATCAA AGAAGATATC CTCATTGACA AACTGAATGA GAATTCGACG 
CCAGAGAAAA CCGATGTGAC ACCTTCCACC ACTTCTCCAC CTTCGTATGA TAGTGTGACC 
AAACCAGAAA AAGAAAAATT TGAAAAAGAC AAATCAGAAA AGGAAGACAA AGGGAAAGAT 
ATCAGGGAAA GTAAAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC478773 ( XM_014110824.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013966299.1 Canis lupus familiaris sodium channel protein type 2 subunit alpha (LOC478773), transcript variant X2, mRNA.

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CloneID	OCe31042
Clone ID Related Accession (Same CDS sequence)	XM_535939.5 , XM_014110824.2 , XM_022414740.1 , XM_014110825.2 , XM_014110824.1 , XM_014110825.1 , XM_535939.6
Accession Version	XM_014110824.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	sodium channel protein type 2 subunit alpha
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726122.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGT CAGTGCTGGT ACCACCAGGA CCTGACAGCT TCCGCTTCTT TACCAGGGAA 
TCCCTTGCTG CTATTGAACA ACGCATTGCA GAAGAGAAAG CTAAGAGACC CAAACAGGAG 
CGTAAGGATG AGGATGATGA AAATGGCCCA AAGCCAAACA GTGACTTGGA AGCAGGGAAA 
TCTCTTCCAT TTATTTATGG AGACATTCCT CCAGAGATGG TGTCAGAGCC CTTGGAGGAC 
CTGGACCCGT ACTATATCAA TAAGAAAACA TTTATAGTAT TGAATAAAGG GAAAGCAATC 
TCTCGATTCA GTGCCACCCC TGCCCTGTAC ATTTTAACTC CCTTCAACCC TATTAGAAAA 
TTAGCTATTA AGATTTTGGT ACATTCTTTA TTCAATGTGC TCATTATGTG CACTATTCTC 
ACCAACTGTG TATTTATGAC CATGAGTAAC CCTCCAGACT GGACAAAGAA TGTGGAGTAC 
ACCTTTACAG GAATTTATAC ATTTGAATCA CTTATAAAAA TACTTGCAAG GGGCTTTTGT 
TTAGAAGATT TCACATTTCT CCGGGATCCA TGGAATTGGT TGGATTTCAC AGTCATTACT 
TTTGCATACG TGACAGAGTT TGTGGATCTG GGCAATGTCT CAGCGCTGAG AACATTCAGA 
GTTCTCCGAG CACTGAAAAC AATTTCAGTC ATTCCAGGCC TGAAGACCAT TGTGGGGGCC 
CTGATCCAGT CGGTGAAGAA GCTCTCTGAT GTAATGATTC TGACTGTGTT CTGTCTGAGT 
GTGTTTGCAC TCATAGGACT GCAGCTGTTT ATGGGCAACC TGCGGAATAA ATGCTTGCAA 
TGGCCCCCAG ATAATTCTTC CTTTGAAATA AACATCACTT CCTTCTTTAA TAATTCATTG 
GATGGGAACG GTACCATGTT CAATAGGACA GTGAGCATGT TTAACTGGGA TGAATATATT 
GAGGATAAAA GTCACTTTTA TTTTTTGGAA GGGCAAAATG ATGCTCTGCT TTGTGGCAAC 
AGCTCAGATG CAGGCCAGTG TCCGGAAGGA TATATCTGTG TGAAGGCTGG TAGAAACCCC 
AACTATGGCT ATACAAGCTT TGATACCTTT AGTTGGGCCT TCTTGTCATT ATTTCGTCTC 
ATGACTCAAG ACTTCTGGGA AAACCTTTAC CAACTGACGC TACGTGCTGC TGGGAAAACA 
TATATGATAT TTTTTGTGCT GGTCATTTTC TTGGGCTCAT TCTACCTTAT AAATTTAATC 
CTGGCTGTGG TGGCTATGGC CTACGAGGAA CAGAATCAGG CAACTCTTGA GGAAGCTGAA 
CAGAAAGAAG CTGAATTTCA GCAGATGCTC GAACAACTAA AAAAACAACA AGAAGAAGCT 
CAGGCAGCAG CTGCAGCCGC ATCTGCAGAA TCAAGAGAGT TCAGTGGTGC TGGTGGGATA 
GGAGTTTTCT CAGAGAGTTC TTCAGTAGCA TCAAAGTTGA GCTCCAAGAG TGAAAAAGAG 
CTGAAAAACA GAAGGAAGAA AAAGAAGCAG AAAGAGCAGT CTGGAGAAGA AGAGAAGGAA 
GATGGGGTTC GCAAATCCGA ATCCGAAGAC AGTATAAGAA GAAAAGGTTT CCGTTTTTCC 
TTAGAAGGAA GCAGGCTGAC ATATGAAAAA AGGTTCTCTT CTCCACATCA GTCTTTATTG 
AGCATCCGTG GCTCCCTTTT CTCTCCAAGA CGCAACAGTA GGGCGAGCCT TTTCAGTTTC 
AGAGGCCGAA CAAAGGATAT TGGCTCTGAG AACGACTTTG CTGATGATGA GCACAGCACC 
TTTGAAGACA ATGACAGCCG GAGAGACTCT CTGTTTGTGC CACACAGACA TGGAGAGCGG 
CGTCATAGCA ATGTCAGCCA GGCCAGCCGG GCCTCCAGGG TGATCCCCAT CCTGCCGGTG 
AATGGAAAGA TGCACAGTGC AGTGGATTGC AACGGGGTCG TCTCGCTGGT TGGGGGCCCT 
TCTGCCCTCA CGTCTCCAGC TGGACAGCTC CTTCCGGAGG GCACGACTAC TGAAACAGAA 
CTAAGAAAGA GACGGTCCAG TTCTTACCAT GTTTCCATGG ATTTGCTGGA AGATCCTACC 
ACAAGGCAAA GAGCAATGAG TCTAGCCAGT ATTTTGACCA ACACCATGGA AGAACTTGAA 
GAATCCAGAC AGAAATGCCC ACCCTGTTGG TATAAATTTG CTAACATGTG TTTGATTTGG 
GACTGCTGTA AACCATGGTT AAAGGTGAAA CATCTCGTCA ACCTGGTTGT AATGGACCCG 
TTTGTTGACC TGGCCATCAC CATCTGCATT GTCCTAAATA CACTGTTTAT GGCCATGGAG 
CACTATCCTA TGACAGAGCA GTTCAGCAGT GTGCTGTCAG TTGGGAACCT GGTCTTCACT 
GGGATCTTCA CAGCAGAAAT GTTCCTCAAG ATAATAGCCA TGGATCCATA TTATTACTTT 
CAAGAGGGCT GGAATATTTT TGATGGTTTA ATCGTAAGCC TTAGTTTAAT GGAACTTGGT 
TTGGCAAACG TTGAAGGATT GTCAGTTCTC CGATCATTTC GACTGCTCCG AGTTTTCAAA 
TTGGCAAAAT CCTGGCCCAC CCTGAATATG CTGATAAAGA TCATCGGCAA CTCAGTGGGG 
GCTCTGGGCA ACCTCACCTT GGTGTTGGCC ATCATCGTCT TCATTTTTGC CGTGGTTGGC 
ATGCAGCTCT TTGGTAAGAG CTACAAAGAA TGTGTCTGCA AGATCTCCAA TGATTGTGAG 
CTCCCACGGT GGCACATGCA TGACTTCTTC CACTCCTTCC TGATTGTATT CCGTGTGTTG 
TGTGGAGAGT GGATAGAAAC CATGTGGGAC TGTATGGAGG TCGCTGGCCA AACCATGTGC 
CTTACTGTCT TCATGATGGT CATGGTGATT GGAAACCTGG TGGTTCTGAA TCTCTTTCTG 
GCCTTGCTCT TGAGTTCATT CAGTTCTGAC AACCTTGCAG CCACTGACGA TGATAATGAA 
ATGAACAATC TCCAGATTGC TGTGGGAAGG ATGCAGAAAG GAATCGATTA TGTTAAAAGA 
AAACTACGTG AATTTATTCA GAAAGCCTTT GTTAGAAAGC AGAAAGCCTT AGATGAAATT 
AAACCTCTTG AAGATCTAAA TAACAAAAAA GACAGCTGTA TTTCCAACCA TACAACTATA 
GAAATAGGAA AAGATCTCAA TTATCTCAAA GATGGAAATG GAACTACCAG TGGCATAGGC 
AGCAGTGTGG AAAAATATGT TGTGGATGAA AGTGATTACA TGTCATTTAT AAACAATCCC 
AGCCTCACTG TGACTGTACC AATTGCTGTC GGGGAATCTG ACTTTGAAAA TTTAAATACT 
GAAGAATTTA GCAGCGAGTC AGATATGGAG GAAAGCAAAG AGAAGCTAAA TGCAACTAGT 
TCATCTGAAG GCAGCACAGT TGATATAGGA GCTCCCGCTG AGGGAGAGCA ACCTGAGGTA 
GAACCCGAGG AATCTCTTGA ACCTGAAGCC TGTTTCACGG AAGACTGTGT ACGGAAGTTC 
AAGTGTTGTC AGATAAGCAT AGAAGAAGGC AAAGGGAAGC TCTGGTGGAA CTTGAGAAAA 
ACTTGCTATA AGATAGTGGA GCACAACTGG TTTGAAACTT TCATTGTCTT CATGATTCTT 
CTCAGCAGTG GGGCACTGGC CTTTGAAGAT ATATATATTG AGCAGCGAAA AACCATTAAG 
ACCATGTTAG AATATGCCGA CAAAGTTTTC ACTTACATAT TCATTCTGGA AATGCTTCTC 
AAGTGGGTCG CATATGGTTT TCAAATGTAT TTCACCAATG CCTGGTGCTG GCTGGATTTC 
CTGATTGTTG ACGTCTCATT GGTTAGCTTG ACTGCAAACG CCTTGGGTTA CTCAGAACTT 
GGCGCCATCA AATCCCTCAG GACACTCAGA GCTCTGAGGC CATTGAGAGC CCTATCCCGG 
TTTGAAGGAA TGAGGGTTGT TGTCAATGCT CTTCTAGGAG CCATTCCATC CATAATGAAT 
GTGCTCCTGG TTTGTCTTAT CTTTTGGCTA ATATTCAGCA TCATGGGAGT AAATCTTTTC 
GCTGGCAAGT TTTACCATTG TATTAACTAC ACCACTGGAG AAATGTTTGA TGTAAGTGTG 
GTCAACAACT ACAGCGAATG TAAAGCCCTC ATAGAGAGCA ATCAAACTGC CAGGTGGAAA 
AATGTGAAAG TAAACTTTGA TAATGTAGGA CTTGGATATC TTTCTCTGCT TCAAGTAGCC 
ACATTTAAGG GATGGATGGA CATCATGTAT GCAGCTGTTG ATTCACGAAA TGTAGAATTA 
CAACCCAAGT ATGAAGACAA TCTGTACATG TACCTTTATT TTGTCATCTT TATTATTTTT 
GGTTCATTCT TTACCCTGAA TCTTTTCATT GGTGTCATCA TAGATAACTT CAACCAACAG 
AAAAAGAAGT TTGGAGGTCA AGACATTTTT ATGACAGAAG AGCAGAAGAA ATACTACAAT 
GCAATGAAAA AACTGGGTTC AAAGAAACCA CAAAAACCCA TACCTCGACC TGCTAATAAA 
TTCCAAGGAA TGGTCTTTGA TTTTGTAACC AAACAAGTCT TTGATATCAG TATCATGATC 
CTCATCTGCC TCAACATGGT CACCATGATG GTGGAAACTG ATGACCAGAG TCAGGAGATG 
ACAAACATTC TGTACTGGAT TAATCTGGTG TTTATTGTTC TGTTCACTGG AGAATGTGTG 
CTGAAATTAA TCTCCCTTCG TTATTACTAT TTCACCATTG GATGGAATAT TTTTGATTTT 
GTGGTGGTCA TTCTCTCTAT CGTAGGCATG TTTCTGGCTG AGCTGATAGA AAAATACTTT 
GTGTCCCCCA CCCTGTTCCG AGTGATCCGG CTCGCCAGGA TCGGTCGAAT CTTGCGTCTG 
ATCAAAGGGG CGAAGGGGAT CCGCACACTG CTCTTTGCTC TGATGATGTC CCTCCCTGCC 
TTGTTTAACA TCGGCCTCCT GCTCTTCCTG GTCATGTTCA TCTACGCCAT CTTTGGAATG 
TCCAACTTTG CCTATGTCAA GAGGGAAGTT GGAATTGATG ACATGTTCAA CTTTGAGACC 
TTTGGCAACA GCATGATCTG CTTGTTCCAA ATTACCACCT CGGCTGGCTG GGATGGATTG 
CTAGCACCTA TTCTTAATAG TGGACCTCCA GACTGTGATC CTGAAAAAGA TCACCCTGGA 
AGCTCAGTTA AGGGAGACTG TGGGAACCCA TCTGTGGGGA TTTTCTTTTT TGTCAGTTAC 
ATCATCATCT CCTTTCTGGT TGTGGTGAAC ATGTACATCG CTGTCATCCT GGAGAACTTC 
AGTGTTGCCA CTGAGGAGAG TGCAGAGCCC CTGAGCGAGG ATGACTTTGA GATGTTCTAT 
GAAGTCTGGG AGAAGTTTGA TCCTGATGCC ACCCAGTTTA TAGAGTTCTC CAAGCTCTCT 
GATTTTGCAG CTGCCCTGGA TCCTCCTCTT CTCATAGCGA AACCAAACAA AGTCCAGCTC 
ATTGCCATGG ACCTGCCCAT GGTCAGTGGG GACCGGATCC ACTGCCTCGA CATTTTATTT 
GCCTTTACAA AGCGTGTTTT GGGTGAGAGT GGAGAGATGG ATGCCCTTAG AATACAGATG 
GAGGAGCGAT TCATGGCATC GAACCCCTCC AAAGTCTCCT ATGAACCCAT CACAACCACT 
CTGAAACGCA AACAAGAGGA GGTGTCTGCT ATCGTTATCC AGAGGGCTTA TAGACGCTAC 
CTCTTGAAGC AGAAAGTTAA AAAGGTTTCA TGTATCTATA AGAAAGACAA AGGCAAAGAA 
GGGGATGGAA CACCTATCAA AGAAGATATC CTCATTGACA AACTGAATGA GAATTCGACG 
CCAGAGAAAA CCGATGTGAC ACCTTCCACC ACTTCTCCAC CTTCGTATGA TAGTGTGACC 
AAACCAGAAA AAGAAAAATT TGAAAAAGAC AAATCAGAAA AGGAAGACAA AGGGAAAGAT 
ATCAGGGAAA GTAAAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013966299.1
CDS	281..6301
Translation	MAQSVLVPPGPDSFRFFTRESLAAIEQRIAEEKAKRPKQERKDEDDENGPKPNSDLEAGKSLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAISRFSATPALYILTPFNPIRKLAIKILVHSLFNVLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKILARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCLQWPPDNSSFEINITSFFNNSLDGNGTMFNRTVSMFNWDEYIEDKSHFYFLEGQNDALLCGNSSDAGQCPEGYICVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMLEQLKKQQEEAQAAAAAASAESREFSGAGGIGVFSESSSVASKLSSKSEKELKNRRKKKKQKEQSGEEEKEDGVRKSESEDSIRRKGFRFSLEGSRLTYEKRFSSPHQSLLSIRGSLFSPRRNSRASLFSFRGRTKDIGSENDFADDEHSTFEDNDSRRDSLFVPHRHGERRHSNVSQASRASRVIPILPVNGKMHSAVDCNGVVSLVGGPSALTSPAGQLLPEGTTTETELRKRRSSSYHVSMDLLEDPTTRQRAMSLASILTNTMEELEESRQKCPPCWYKFANMCLIWDCCKPWLKVKHLVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTEQFSSVLSVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGLIVSLSLMELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKISNDCELPRWHMHDFFHSFLIVFRVLCGEWIETMWDCMEVAGQTMCLTVFMMVMVIGNLVVLNLFLALLLSSFSSDNLAATDDDNEMNNLQIAVGRMQKGIDYVKRKLREFIQKAFVRKQKALDEIKPLEDLNNKKDSCISNHTTIEIGKDLNYLKDGNGTTSGIGSSVEKYVVDESDYMSFINNPSLTVTVPIAVGESDFENLNTEEFSSESDMEESKEKLNATSSSEGSTVDIGAPAEGEQPEVEPEESLEPEACFTEDCVRKFKCCQISIEEGKGKLWWNLRKTCYKIVEHNWFETFIVFMILLSSGALAFEDIYIEQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGFQMYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINYTTGEMFDVSVVNNYSECKALIESNQTARWKNVKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEDNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPANKFQGMVFDFVTKQVFDISIMILICLNMVTMMVETDDQSQEMTNILYWINLVFIVLFTGECVLKLISLRYYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSGPPDCDPEKDHPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFIEFSKLSDFAAALDPPLLIAKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYEPITTTLKRKQEEVSAIVIQRAYRRYLLKQKVKKVSCIYKKDKGKEGDGTPIKEDILIDKLNENSTPEKTDVTPSTTSPPSYDSVTKPEKEKFEKDKSEKEDKGKDIRESKK

Target ORF information:

RefSeq Version	XM_014110824.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris sodium channel protein type 2 subunit alpha (LOC478773), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014110824.1

ORF Insert Sequence:

ATGGCACAGT CAGTGCTGGT ACCACCAGGA CCTGACAGCT TCCGCTTCTT TACCAGGGAA 
TCCCTTGCTG CTATTGAACA ACGCATTGCA GAAGAGAAAG CTAAGAGACC CAAACAGGAG 
CGTAAGGATG AGGATGATGA AAATGGCCCA AAGCCAAACA GTGACTTGGA AGCAGGGAAA 
TCTCTTCCAT TTATTTATGG AGACATTCCT CCAGAGATGG TGTCAGAGCC CTTGGAGGAC 
CTGGACCCGT ACTATATCAA TAAGAAAACA TTTATAGTAT TGAATAAAGG GAAAGCAATC 
TCTCGATTCA GTGCCACCCC TGCCCTGTAC ATTTTAACTC CCTTCAACCC TATTAGAAAA 
TTAGCTATTA AGATTTTGGT ACATTCTTTA TTCAATGTGC TCATTATGTG CACTATTCTC 
ACCAACTGTG TATTTATGAC CATGAGTAAC CCTCCAGACT GGACAAAGAA TGTGGAGTAC 
ACCTTTACAG GAATTTATAC ATTTGAATCA CTTATAAAAA TACTTGCAAG GGGCTTTTGT 
TTAGAAGATT TCACATTTCT CCGGGATCCA TGGAATTGGT TGGATTTCAC AGTCATTACT 
TTTGCATACG TGACAGAGTT TGTGGATCTG GGCAATGTCT CAGCGCTGAG AACATTCAGA 
GTTCTCCGAG CACTGAAAAC AATTTCAGTC ATTCCAGGCC TGAAGACCAT TGTGGGGGCC 
CTGATCCAGT CGGTGAAGAA GCTCTCTGAT GTAATGATTC TGACTGTGTT CTGTCTGAGT 
GTGTTTGCAC TCATAGGACT GCAGCTGTTT ATGGGCAACC TGCGGAATAA ATGCTTGCAA 
TGGCCCCCAG ATAATTCTTC CTTTGAAATA AACATCACTT CCTTCTTTAA TAATTCATTG 
GATGGGAACG GTACCATGTT CAATAGGACA GTGAGCATGT TTAACTGGGA TGAATATATT 
GAGGATAAAA GTCACTTTTA TTTTTTGGAA GGGCAAAATG ATGCTCTGCT TTGTGGCAAC 
AGCTCAGATG CAGGCCAGTG TCCGGAAGGA TATATCTGTG TGAAGGCTGG TAGAAACCCC 
AACTATGGCT ATACAAGCTT TGATACCTTT AGTTGGGCCT TCTTGTCATT ATTTCGTCTC 
ATGACTCAAG ACTTCTGGGA AAACCTTTAC CAACTGACGC TACGTGCTGC TGGGAAAACA 
TATATGATAT TTTTTGTGCT GGTCATTTTC TTGGGCTCAT TCTACCTTAT AAATTTAATC 
CTGGCTGTGG TGGCTATGGC CTACGAGGAA CAGAATCAGG CAACTCTTGA GGAAGCTGAA 
CAGAAAGAAG CTGAATTTCA GCAGATGCTC GAACAACTAA AAAAACAACA AGAAGAAGCT 
CAGGCAGCAG CTGCAGCCGC ATCTGCAGAA TCAAGAGAGT TCAGTGGTGC TGGTGGGATA 
GGAGTTTTCT CAGAGAGTTC TTCAGTAGCA TCAAAGTTGA GCTCCAAGAG TGAAAAAGAG 
CTGAAAAACA GAAGGAAGAA AAAGAAGCAG AAAGAGCAGT CTGGAGAAGA AGAGAAGGAA 
GATGGGGTTC GCAAATCCGA ATCCGAAGAC AGTATAAGAA GAAAAGGTTT CCGTTTTTCC 
TTAGAAGGAA GCAGGCTGAC ATATGAAAAA AGGTTCTCTT CTCCACATCA GTCTTTATTG 
AGCATCCGTG GCTCCCTTTT CTCTCCAAGA CGCAACAGTA GGGCGAGCCT TTTCAGTTTC 
AGAGGCCGAA CAAAGGATAT TGGCTCTGAG AACGACTTTG CTGATGATGA GCACAGCACC 
TTTGAAGACA ATGACAGCCG GAGAGACTCT CTGTTTGTGC CACACAGACA TGGAGAGCGG 
CGTCATAGCA ATGTCAGCCA GGCCAGCCGG GCCTCCAGGG TGATCCCCAT CCTGCCGGTG 
AATGGAAAGA TGCACAGTGC AGTGGATTGC AACGGGGTCG TCTCGCTGGT TGGGGGCCCT 
TCTGCCCTCA CGTCTCCAGC TGGACAGCTC CTTCCGGAGG GCACGACTAC TGAAACAGAA 
CTAAGAAAGA GACGGTCCAG TTCTTACCAT GTTTCCATGG ATTTGCTGGA AGATCCTACC 
ACAAGGCAAA GAGCAATGAG TCTAGCCAGT ATTTTGACCA ACACCATGGA AGAACTTGAA 
GAATCCAGAC AGAAATGCCC ACCCTGTTGG TATAAATTTG CTAACATGTG TTTGATTTGG 
GACTGCTGTA AACCATGGTT AAAGGTGAAA CATCTCGTCA ACCTGGTTGT AATGGACCCG 
TTTGTTGACC TGGCCATCAC CATCTGCATT GTCCTAAATA CACTGTTTAT GGCCATGGAG 
CACTATCCTA TGACAGAGCA GTTCAGCAGT GTGCTGTCAG TTGGGAACCT GGTCTTCACT 
GGGATCTTCA CAGCAGAAAT GTTCCTCAAG ATAATAGCCA TGGATCCATA TTATTACTTT 
CAAGAGGGCT GGAATATTTT TGATGGTTTA ATCGTAAGCC TTAGTTTAAT GGAACTTGGT 
TTGGCAAACG TTGAAGGATT GTCAGTTCTC CGATCATTTC GACTGCTCCG AGTTTTCAAA 
TTGGCAAAAT CCTGGCCCAC CCTGAATATG CTGATAAAGA TCATCGGCAA CTCAGTGGGG 
GCTCTGGGCA ACCTCACCTT GGTGTTGGCC ATCATCGTCT TCATTTTTGC CGTGGTTGGC 
ATGCAGCTCT TTGGTAAGAG CTACAAAGAA TGTGTCTGCA AGATCTCCAA TGATTGTGAG 
CTCCCACGGT GGCACATGCA TGACTTCTTC CACTCCTTCC TGATTGTATT CCGTGTGTTG 
TGTGGAGAGT GGATAGAAAC CATGTGGGAC TGTATGGAGG TCGCTGGCCA AACCATGTGC 
CTTACTGTCT TCATGATGGT CATGGTGATT GGAAACCTGG TGGTTCTGAA TCTCTTTCTG 
GCCTTGCTCT TGAGTTCATT CAGTTCTGAC AACCTTGCAG CCACTGACGA TGATAATGAA 
ATGAACAATC TCCAGATTGC TGTGGGAAGG ATGCAGAAAG GAATCGATTA TGTTAAAAGA 
AAACTACGTG AATTTATTCA GAAAGCCTTT GTTAGAAAGC AGAAAGCCTT AGATGAAATT 
AAACCTCTTG AAGATCTAAA TAACAAAAAA GACAGCTGTA TTTCCAACCA TACAACTATA 
GAAATAGGAA AAGATCTCAA TTATCTCAAA GATGGAAATG GAACTACCAG TGGCATAGGC 
AGCAGTGTGG AAAAATATGT TGTGGATGAA AGTGATTACA TGTCATTTAT AAACAATCCC 
AGCCTCACTG TGACTGTACC AATTGCTGTC GGGGAATCTG ACTTTGAAAA TTTAAATACT 
GAAGAATTTA GCAGCGAGTC AGATATGGAG GAAAGCAAAG AGAAGCTAAA TGCAACTAGT 
TCATCTGAAG GCAGCACAGT TGATATAGGA GCTCCCGCTG AGGGAGAGCA ACCTGAGGTA 
GAACCCGAGG AATCTCTTGA ACCTGAAGCC TGTTTCACGG AAGACTGTGT ACGGAAGTTC 
AAGTGTTGTC AGATAAGCAT AGAAGAAGGC AAAGGGAAGC TCTGGTGGAA CTTGAGAAAA 
ACTTGCTATA AGATAGTGGA GCACAACTGG TTTGAAACTT TCATTGTCTT CATGATTCTT 
CTCAGCAGTG GGGCACTGGC CTTTGAAGAT ATATATATTG AGCAGCGAAA AACCATTAAG 
ACCATGTTAG AATATGCCGA CAAAGTTTTC ACTTACATAT TCATTCTGGA AATGCTTCTC 
AAGTGGGTCG CATATGGTTT TCAAATGTAT TTCACCAATG CCTGGTGCTG GCTGGATTTC 
CTGATTGTTG ACGTCTCATT GGTTAGCTTG ACTGCAAACG CCTTGGGTTA CTCAGAACTT 
GGCGCCATCA AATCCCTCAG GACACTCAGA GCTCTGAGGC CATTGAGAGC CCTATCCCGG 
TTTGAAGGAA TGAGGGTTGT TGTCAATGCT CTTCTAGGAG CCATTCCATC CATAATGAAT 
GTGCTCCTGG TTTGTCTTAT CTTTTGGCTA ATATTCAGCA TCATGGGAGT AAATCTTTTC 
GCTGGCAAGT TTTACCATTG TATTAACTAC ACCACTGGAG AAATGTTTGA TGTAAGTGTG 
GTCAACAACT ACAGCGAATG TAAAGCCCTC ATAGAGAGCA ATCAAACTGC CAGGTGGAAA 
AATGTGAAAG TAAACTTTGA TAATGTAGGA CTTGGATATC TTTCTCTGCT TCAAGTAGCC 
ACATTTAAGG GATGGATGGA CATCATGTAT GCAGCTGTTG ATTCACGAAA TGTAGAATTA 
CAACCCAAGT ATGAAGACAA TCTGTACATG TACCTTTATT TTGTCATCTT TATTATTTTT 
GGTTCATTCT TTACCCTGAA TCTTTTCATT GGTGTCATCA TAGATAACTT CAACCAACAG 
AAAAAGAAGT TTGGAGGTCA AGACATTTTT ATGACAGAAG AGCAGAAGAA ATACTACAAT 
GCAATGAAAA AACTGGGTTC AAAGAAACCA CAAAAACCCA TACCTCGACC TGCTAATAAA 
TTCCAAGGAA TGGTCTTTGA TTTTGTAACC AAACAAGTCT TTGATATCAG TATCATGATC 
CTCATCTGCC TCAACATGGT CACCATGATG GTGGAAACTG ATGACCAGAG TCAGGAGATG 
ACAAACATTC TGTACTGGAT TAATCTGGTG TTTATTGTTC TGTTCACTGG AGAATGTGTG 
CTGAAATTAA TCTCCCTTCG TTATTACTAT TTCACCATTG GATGGAATAT TTTTGATTTT 
GTGGTGGTCA TTCTCTCTAT CGTAGGCATG TTTCTGGCTG AGCTGATAGA AAAATACTTT 
GTGTCCCCCA CCCTGTTCCG AGTGATCCGG CTCGCCAGGA TCGGTCGAAT CTTGCGTCTG 
ATCAAAGGGG CGAAGGGGAT CCGCACACTG CTCTTTGCTC TGATGATGTC CCTCCCTGCC 
TTGTTTAACA TCGGCCTCCT GCTCTTCCTG GTCATGTTCA TCTACGCCAT CTTTGGAATG 
TCCAACTTTG CCTATGTCAA GAGGGAAGTT GGAATTGATG ACATGTTCAA CTTTGAGACC 
TTTGGCAACA GCATGATCTG CTTGTTCCAA ATTACCACCT CGGCTGGCTG GGATGGATTG 
CTAGCACCTA TTCTTAATAG TGGACCTCCA GACTGTGATC CTGAAAAAGA TCACCCTGGA 
AGCTCAGTTA AGGGAGACTG TGGGAACCCA TCTGTGGGGA TTTTCTTTTT TGTCAGTTAC 
ATCATCATCT CCTTTCTGGT TGTGGTGAAC ATGTACATCG CTGTCATCCT GGAGAACTTC 
AGTGTTGCCA CTGAGGAGAG TGCAGAGCCC CTGAGCGAGG ATGACTTTGA GATGTTCTAT 
GAAGTCTGGG AGAAGTTTGA TCCTGATGCC ACCCAGTTTA TAGAGTTCTC CAAGCTCTCT 
GATTTTGCAG CTGCCCTGGA TCCTCCTCTT CTCATAGCGA AACCAAACAA AGTCCAGCTC 
ATTGCCATGG ACCTGCCCAT GGTCAGTGGG GACCGGATCC ACTGCCTCGA CATTTTATTT 
GCCTTTACAA AGCGTGTTTT GGGTGAGAGT GGAGAGATGG ATGCCCTTAG AATACAGATG 
GAGGAGCGAT TCATGGCATC GAACCCCTCC AAAGTCTCCT ATGAACCCAT CACAACCACT 
CTGAAACGCA AACAAGAGGA GGTGTCTGCT ATCGTTATCC AGAGGGCTTA TAGACGCTAC 
CTCTTGAAGC AGAAAGTTAA AAAGGTTTCA TGTATCTATA AGAAAGACAA AGGCAAAGAA 
GGGGATGGAA CACCTATCAA AGAAGATATC CTCATTGACA AACTGAATGA GAATTCGACG 
CCAGAGAAAA CCGATGTGAC ACCTTCCACC ACTTCTCCAC CTTCGTATGA TAGTGTGACC 
AAACCAGAAA AAGAAAAATT TGAAAAAGAC AAATCAGAAA AGGAAGACAA AGGGAAAGAT 
ATCAGGGAAA GTAAAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC478773 ( XM_014110825.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013966300.1 Canis lupus familiaris sodium channel protein type 2 subunit alpha (LOC478773), transcript variant X3, mRNA.

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CloneID	OCe31042
Clone ID Related Accession (Same CDS sequence)	XM_535939.5 , XM_014110824.2 , XM_022414740.1 , XM_014110825.2 , XM_014110824.1 , XM_014110825.1 , XM_535939.6
Accession Version	XM_014110825.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	sodium channel protein type 2 subunit alpha
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726122.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGT CAGTGCTGGT ACCACCAGGA CCTGACAGCT TCCGCTTCTT TACCAGGGAA 
TCCCTTGCTG CTATTGAACA ACGCATTGCA GAAGAGAAAG CTAAGAGACC CAAACAGGAG 
CGTAAGGATG AGGATGATGA AAATGGCCCA AAGCCAAACA GTGACTTGGA AGCAGGGAAA 
TCTCTTCCAT TTATTTATGG AGACATTCCT CCAGAGATGG TGTCAGAGCC CTTGGAGGAC 
CTGGACCCGT ACTATATCAA TAAGAAAACA TTTATAGTAT TGAATAAAGG GAAAGCAATC 
TCTCGATTCA GTGCCACCCC TGCCCTGTAC ATTTTAACTC CCTTCAACCC TATTAGAAAA 
TTAGCTATTA AGATTTTGGT ACATTCTTTA TTCAATGTGC TCATTATGTG CACTATTCTC 
ACCAACTGTG TATTTATGAC CATGAGTAAC CCTCCAGACT GGACAAAGAA TGTGGAGTAC 
ACCTTTACAG GAATTTATAC ATTTGAATCA CTTATAAAAA TACTTGCAAG GGGCTTTTGT 
TTAGAAGATT TCACATTTCT CCGGGATCCA TGGAATTGGT TGGATTTCAC AGTCATTACT 
TTTGCATACG TGACAGAGTT TGTGGATCTG GGCAATGTCT CAGCGCTGAG AACATTCAGA 
GTTCTCCGAG CACTGAAAAC AATTTCAGTC ATTCCAGGCC TGAAGACCAT TGTGGGGGCC 
CTGATCCAGT CGGTGAAGAA GCTCTCTGAT GTAATGATTC TGACTGTGTT CTGTCTGAGT 
GTGTTTGCAC TCATAGGACT GCAGCTGTTT ATGGGCAACC TGCGGAATAA ATGCTTGCAA 
TGGCCCCCAG ATAATTCTTC CTTTGAAATA AACATCACTT CCTTCTTTAA TAATTCATTG 
GATGGGAACG GTACCATGTT CAATAGGACA GTGAGCATGT TTAACTGGGA TGAATATATT 
GAGGATAAAA GTCACTTTTA TTTTTTGGAA GGGCAAAATG ATGCTCTGCT TTGTGGCAAC 
AGCTCAGATG CAGGCCAGTG TCCGGAAGGA TATATCTGTG TGAAGGCTGG TAGAAACCCC 
AACTATGGCT ATACAAGCTT TGATACCTTT AGTTGGGCCT TCTTGTCATT ATTTCGTCTC 
ATGACTCAAG ACTTCTGGGA AAACCTTTAC CAACTGACGC TACGTGCTGC TGGGAAAACA 
TATATGATAT TTTTTGTGCT GGTCATTTTC TTGGGCTCAT TCTACCTTAT AAATTTAATC 
CTGGCTGTGG TGGCTATGGC CTACGAGGAA CAGAATCAGG CAACTCTTGA GGAAGCTGAA 
CAGAAAGAAG CTGAATTTCA GCAGATGCTC GAACAACTAA AAAAACAACA AGAAGAAGCT 
CAGGCAGCAG CTGCAGCCGC ATCTGCAGAA TCAAGAGAGT TCAGTGGTGC TGGTGGGATA 
GGAGTTTTCT CAGAGAGTTC TTCAGTAGCA TCAAAGTTGA GCTCCAAGAG TGAAAAAGAG 
CTGAAAAACA GAAGGAAGAA AAAGAAGCAG AAAGAGCAGT CTGGAGAAGA AGAGAAGGAA 
GATGGGGTTC GCAAATCCGA ATCCGAAGAC AGTATAAGAA GAAAAGGTTT CCGTTTTTCC 
TTAGAAGGAA GCAGGCTGAC ATATGAAAAA AGGTTCTCTT CTCCACATCA GTCTTTATTG 
AGCATCCGTG GCTCCCTTTT CTCTCCAAGA CGCAACAGTA GGGCGAGCCT TTTCAGTTTC 
AGAGGCCGAA CAAAGGATAT TGGCTCTGAG AACGACTTTG CTGATGATGA GCACAGCACC 
TTTGAAGACA ATGACAGCCG GAGAGACTCT CTGTTTGTGC CACACAGACA TGGAGAGCGG 
CGTCATAGCA ATGTCAGCCA GGCCAGCCGG GCCTCCAGGG TGATCCCCAT CCTGCCGGTG 
AATGGAAAGA TGCACAGTGC AGTGGATTGC AACGGGGTCG TCTCGCTGGT TGGGGGCCCT 
TCTGCCCTCA CGTCTCCAGC TGGACAGCTC CTTCCGGAGG GCACGACTAC TGAAACAGAA 
CTAAGAAAGA GACGGTCCAG TTCTTACCAT GTTTCCATGG ATTTGCTGGA AGATCCTACC 
ACAAGGCAAA GAGCAATGAG TCTAGCCAGT ATTTTGACCA ACACCATGGA AGAACTTGAA 
GAATCCAGAC AGAAATGCCC ACCCTGTTGG TATAAATTTG CTAACATGTG TTTGATTTGG 
GACTGCTGTA AACCATGGTT AAAGGTGAAA CATCTCGTCA ACCTGGTTGT AATGGACCCG 
TTTGTTGACC TGGCCATCAC CATCTGCATT GTCCTAAATA CACTGTTTAT GGCCATGGAG 
CACTATCCTA TGACAGAGCA GTTCAGCAGT GTGCTGTCAG TTGGGAACCT GGTCTTCACT 
GGGATCTTCA CAGCAGAAAT GTTCCTCAAG ATAATAGCCA TGGATCCATA TTATTACTTT 
CAAGAGGGCT GGAATATTTT TGATGGTTTA ATCGTAAGCC TTAGTTTAAT GGAACTTGGT 
TTGGCAAACG TTGAAGGATT GTCAGTTCTC CGATCATTTC GACTGCTCCG AGTTTTCAAA 
TTGGCAAAAT CCTGGCCCAC CCTGAATATG CTGATAAAGA TCATCGGCAA CTCAGTGGGG 
GCTCTGGGCA ACCTCACCTT GGTGTTGGCC ATCATCGTCT TCATTTTTGC CGTGGTTGGC 
ATGCAGCTCT TTGGTAAGAG CTACAAAGAA TGTGTCTGCA AGATCTCCAA TGATTGTGAG 
CTCCCACGGT GGCACATGCA TGACTTCTTC CACTCCTTCC TGATTGTATT CCGTGTGTTG 
TGTGGAGAGT GGATAGAAAC CATGTGGGAC TGTATGGAGG TCGCTGGCCA AACCATGTGC 
CTTACTGTCT TCATGATGGT CATGGTGATT GGAAACCTGG TGGTTCTGAA TCTCTTTCTG 
GCCTTGCTCT TGAGTTCATT CAGTTCTGAC AACCTTGCAG CCACTGACGA TGATAATGAA 
ATGAACAATC TCCAGATTGC TGTGGGAAGG ATGCAGAAAG GAATCGATTA TGTTAAAAGA 
AAACTACGTG AATTTATTCA GAAAGCCTTT GTTAGAAAGC AGAAAGCCTT AGATGAAATT 
AAACCTCTTG AAGATCTAAA TAACAAAAAA GACAGCTGTA TTTCCAACCA TACAACTATA 
GAAATAGGAA AAGATCTCAA TTATCTCAAA GATGGAAATG GAACTACCAG TGGCATAGGC 
AGCAGTGTGG AAAAATATGT TGTGGATGAA AGTGATTACA TGTCATTTAT AAACAATCCC 
AGCCTCACTG TGACTGTACC AATTGCTGTC GGGGAATCTG ACTTTGAAAA TTTAAATACT 
GAAGAATTTA GCAGCGAGTC AGATATGGAG GAAAGCAAAG AGAAGCTAAA TGCAACTAGT 
TCATCTGAAG GCAGCACAGT TGATATAGGA GCTCCCGCTG AGGGAGAGCA ACCTGAGGTA 
GAACCCGAGG AATCTCTTGA ACCTGAAGCC TGTTTCACGG AAGACTGTGT ACGGAAGTTC 
AAGTGTTGTC AGATAAGCAT AGAAGAAGGC AAAGGGAAGC TCTGGTGGAA CTTGAGAAAA 
ACTTGCTATA AGATAGTGGA GCACAACTGG TTTGAAACTT TCATTGTCTT CATGATTCTT 
CTCAGCAGTG GGGCACTGGC CTTTGAAGAT ATATATATTG AGCAGCGAAA AACCATTAAG 
ACCATGTTAG AATATGCCGA CAAAGTTTTC ACTTACATAT TCATTCTGGA AATGCTTCTC 
AAGTGGGTCG CATATGGTTT TCAAATGTAT TTCACCAATG CCTGGTGCTG GCTGGATTTC 
CTGATTGTTG ACGTCTCATT GGTTAGCTTG ACTGCAAACG CCTTGGGTTA CTCAGAACTT 
GGCGCCATCA AATCCCTCAG GACACTCAGA GCTCTGAGGC CATTGAGAGC CCTATCCCGG 
TTTGAAGGAA TGAGGGTTGT TGTCAATGCT CTTCTAGGAG CCATTCCATC CATAATGAAT 
GTGCTCCTGG TTTGTCTTAT CTTTTGGCTA ATATTCAGCA TCATGGGAGT AAATCTTTTC 
GCTGGCAAGT TTTACCATTG TATTAACTAC ACCACTGGAG AAATGTTTGA TGTAAGTGTG 
GTCAACAACT ACAGCGAATG TAAAGCCCTC ATAGAGAGCA ATCAAACTGC CAGGTGGAAA 
AATGTGAAAG TAAACTTTGA TAATGTAGGA CTTGGATATC TTTCTCTGCT TCAAGTAGCC 
ACATTTAAGG GATGGATGGA CATCATGTAT GCAGCTGTTG ATTCACGAAA TGTAGAATTA 
CAACCCAAGT ATGAAGACAA TCTGTACATG TACCTTTATT TTGTCATCTT TATTATTTTT 
GGTTCATTCT TTACCCTGAA TCTTTTCATT GGTGTCATCA TAGATAACTT CAACCAACAG 
AAAAAGAAGT TTGGAGGTCA AGACATTTTT ATGACAGAAG AGCAGAAGAA ATACTACAAT 
GCAATGAAAA AACTGGGTTC AAAGAAACCA CAAAAACCCA TACCTCGACC TGCTAATAAA 
TTCCAAGGAA TGGTCTTTGA TTTTGTAACC AAACAAGTCT TTGATATCAG TATCATGATC 
CTCATCTGCC TCAACATGGT CACCATGATG GTGGAAACTG ATGACCAGAG TCAGGAGATG 
ACAAACATTC TGTACTGGAT TAATCTGGTG TTTATTGTTC TGTTCACTGG AGAATGTGTG 
CTGAAATTAA TCTCCCTTCG TTATTACTAT TTCACCATTG GATGGAATAT TTTTGATTTT 
GTGGTGGTCA TTCTCTCTAT CGTAGGCATG TTTCTGGCTG AGCTGATAGA AAAATACTTT 
GTGTCCCCCA CCCTGTTCCG AGTGATCCGG CTCGCCAGGA TCGGTCGAAT CTTGCGTCTG 
ATCAAAGGGG CGAAGGGGAT CCGCACACTG CTCTTTGCTC TGATGATGTC CCTCCCTGCC 
TTGTTTAACA TCGGCCTCCT GCTCTTCCTG GTCATGTTCA TCTACGCCAT CTTTGGAATG 
TCCAACTTTG CCTATGTCAA GAGGGAAGTT GGAATTGATG ACATGTTCAA CTTTGAGACC 
TTTGGCAACA GCATGATCTG CTTGTTCCAA ATTACCACCT CGGCTGGCTG GGATGGATTG 
CTAGCACCTA TTCTTAATAG TGGACCTCCA GACTGTGATC CTGAAAAAGA TCACCCTGGA 
AGCTCAGTTA AGGGAGACTG TGGGAACCCA TCTGTGGGGA TTTTCTTTTT TGTCAGTTAC 
ATCATCATCT CCTTTCTGGT TGTGGTGAAC ATGTACATCG CTGTCATCCT GGAGAACTTC 
AGTGTTGCCA CTGAGGAGAG TGCAGAGCCC CTGAGCGAGG ATGACTTTGA GATGTTCTAT 
GAAGTCTGGG AGAAGTTTGA TCCTGATGCC ACCCAGTTTA TAGAGTTCTC CAAGCTCTCT 
GATTTTGCAG CTGCCCTGGA TCCTCCTCTT CTCATAGCGA AACCAAACAA AGTCCAGCTC 
ATTGCCATGG ACCTGCCCAT GGTCAGTGGG GACCGGATCC ACTGCCTCGA CATTTTATTT 
GCCTTTACAA AGCGTGTTTT GGGTGAGAGT GGAGAGATGG ATGCCCTTAG AATACAGATG 
GAGGAGCGAT TCATGGCATC GAACCCCTCC AAAGTCTCCT ATGAACCCAT CACAACCACT 
CTGAAACGCA AACAAGAGGA GGTGTCTGCT ATCGTTATCC AGAGGGCTTA TAGACGCTAC 
CTCTTGAAGC AGAAAGTTAA AAAGGTTTCA TGTATCTATA AGAAAGACAA AGGCAAAGAA 
GGGGATGGAA CACCTATCAA AGAAGATATC CTCATTGACA AACTGAATGA GAATTCGACG 
CCAGAGAAAA CCGATGTGAC ACCTTCCACC ACTTCTCCAC CTTCGTATGA TAGTGTGACC 
AAACCAGAAA AAGAAAAATT TGAAAAAGAC AAATCAGAAA AGGAAGACAA AGGGAAAGAT 
ATCAGGGAAA GTAAAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013966300.1
CDS	137..6157
Translation	MAQSVLVPPGPDSFRFFTRESLAAIEQRIAEEKAKRPKQERKDEDDENGPKPNSDLEAGKSLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAISRFSATPALYILTPFNPIRKLAIKILVHSLFNVLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKILARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCLQWPPDNSSFEINITSFFNNSLDGNGTMFNRTVSMFNWDEYIEDKSHFYFLEGQNDALLCGNSSDAGQCPEGYICVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMLEQLKKQQEEAQAAAAAASAESREFSGAGGIGVFSESSSVASKLSSKSEKELKNRRKKKKQKEQSGEEEKEDGVRKSESEDSIRRKGFRFSLEGSRLTYEKRFSSPHQSLLSIRGSLFSPRRNSRASLFSFRGRTKDIGSENDFADDEHSTFEDNDSRRDSLFVPHRHGERRHSNVSQASRASRVIPILPVNGKMHSAVDCNGVVSLVGGPSALTSPAGQLLPEGTTTETELRKRRSSSYHVSMDLLEDPTTRQRAMSLASILTNTMEELEESRQKCPPCWYKFANMCLIWDCCKPWLKVKHLVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTEQFSSVLSVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGLIVSLSLMELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKISNDCELPRWHMHDFFHSFLIVFRVLCGEWIETMWDCMEVAGQTMCLTVFMMVMVIGNLVVLNLFLALLLSSFSSDNLAATDDDNEMNNLQIAVGRMQKGIDYVKRKLREFIQKAFVRKQKALDEIKPLEDLNNKKDSCISNHTTIEIGKDLNYLKDGNGTTSGIGSSVEKYVVDESDYMSFINNPSLTVTVPIAVGESDFENLNTEEFSSESDMEESKEKLNATSSSEGSTVDIGAPAEGEQPEVEPEESLEPEACFTEDCVRKFKCCQISIEEGKGKLWWNLRKTCYKIVEHNWFETFIVFMILLSSGALAFEDIYIEQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGFQMYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINYTTGEMFDVSVVNNYSECKALIESNQTARWKNVKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEDNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPANKFQGMVFDFVTKQVFDISIMILICLNMVTMMVETDDQSQEMTNILYWINLVFIVLFTGECVLKLISLRYYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSGPPDCDPEKDHPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFIEFSKLSDFAAALDPPLLIAKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYEPITTTLKRKQEEVSAIVIQRAYRRYLLKQKVKKVSCIYKKDKGKEGDGTPIKEDILIDKLNENSTPEKTDVTPSTTSPPSYDSVTKPEKEKFEKDKSEKEDKGKDIRESKK

Target ORF information:

RefSeq Version	XM_014110825.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris sodium channel protein type 2 subunit alpha (LOC478773), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014110825.1

ORF Insert Sequence:

ATGGCACAGT CAGTGCTGGT ACCACCAGGA CCTGACAGCT TCCGCTTCTT TACCAGGGAA 
TCCCTTGCTG CTATTGAACA ACGCATTGCA GAAGAGAAAG CTAAGAGACC CAAACAGGAG 
CGTAAGGATG AGGATGATGA AAATGGCCCA AAGCCAAACA GTGACTTGGA AGCAGGGAAA 
TCTCTTCCAT TTATTTATGG AGACATTCCT CCAGAGATGG TGTCAGAGCC CTTGGAGGAC 
CTGGACCCGT ACTATATCAA TAAGAAAACA TTTATAGTAT TGAATAAAGG GAAAGCAATC 
TCTCGATTCA GTGCCACCCC TGCCCTGTAC ATTTTAACTC CCTTCAACCC TATTAGAAAA 
TTAGCTATTA AGATTTTGGT ACATTCTTTA TTCAATGTGC TCATTATGTG CACTATTCTC 
ACCAACTGTG TATTTATGAC CATGAGTAAC CCTCCAGACT GGACAAAGAA TGTGGAGTAC 
ACCTTTACAG GAATTTATAC ATTTGAATCA CTTATAAAAA TACTTGCAAG GGGCTTTTGT 
TTAGAAGATT TCACATTTCT CCGGGATCCA TGGAATTGGT TGGATTTCAC AGTCATTACT 
TTTGCATACG TGACAGAGTT TGTGGATCTG GGCAATGTCT CAGCGCTGAG AACATTCAGA 
GTTCTCCGAG CACTGAAAAC AATTTCAGTC ATTCCAGGCC TGAAGACCAT TGTGGGGGCC 
CTGATCCAGT CGGTGAAGAA GCTCTCTGAT GTAATGATTC TGACTGTGTT CTGTCTGAGT 
GTGTTTGCAC TCATAGGACT GCAGCTGTTT ATGGGCAACC TGCGGAATAA ATGCTTGCAA 
TGGCCCCCAG ATAATTCTTC CTTTGAAATA AACATCACTT CCTTCTTTAA TAATTCATTG 
GATGGGAACG GTACCATGTT CAATAGGACA GTGAGCATGT TTAACTGGGA TGAATATATT 
GAGGATAAAA GTCACTTTTA TTTTTTGGAA GGGCAAAATG ATGCTCTGCT TTGTGGCAAC 
AGCTCAGATG CAGGCCAGTG TCCGGAAGGA TATATCTGTG TGAAGGCTGG TAGAAACCCC 
AACTATGGCT ATACAAGCTT TGATACCTTT AGTTGGGCCT TCTTGTCATT ATTTCGTCTC 
ATGACTCAAG ACTTCTGGGA AAACCTTTAC CAACTGACGC TACGTGCTGC TGGGAAAACA 
TATATGATAT TTTTTGTGCT GGTCATTTTC TTGGGCTCAT TCTACCTTAT AAATTTAATC 
CTGGCTGTGG TGGCTATGGC CTACGAGGAA CAGAATCAGG CAACTCTTGA GGAAGCTGAA 
CAGAAAGAAG CTGAATTTCA GCAGATGCTC GAACAACTAA AAAAACAACA AGAAGAAGCT 
CAGGCAGCAG CTGCAGCCGC ATCTGCAGAA TCAAGAGAGT TCAGTGGTGC TGGTGGGATA 
GGAGTTTTCT CAGAGAGTTC TTCAGTAGCA TCAAAGTTGA GCTCCAAGAG TGAAAAAGAG 
CTGAAAAACA GAAGGAAGAA AAAGAAGCAG AAAGAGCAGT CTGGAGAAGA AGAGAAGGAA 
GATGGGGTTC GCAAATCCGA ATCCGAAGAC AGTATAAGAA GAAAAGGTTT CCGTTTTTCC 
TTAGAAGGAA GCAGGCTGAC ATATGAAAAA AGGTTCTCTT CTCCACATCA GTCTTTATTG 
AGCATCCGTG GCTCCCTTTT CTCTCCAAGA CGCAACAGTA GGGCGAGCCT TTTCAGTTTC 
AGAGGCCGAA CAAAGGATAT TGGCTCTGAG AACGACTTTG CTGATGATGA GCACAGCACC 
TTTGAAGACA ATGACAGCCG GAGAGACTCT CTGTTTGTGC CACACAGACA TGGAGAGCGG 
CGTCATAGCA ATGTCAGCCA GGCCAGCCGG GCCTCCAGGG TGATCCCCAT CCTGCCGGTG 
AATGGAAAGA TGCACAGTGC AGTGGATTGC AACGGGGTCG TCTCGCTGGT TGGGGGCCCT 
TCTGCCCTCA CGTCTCCAGC TGGACAGCTC CTTCCGGAGG GCACGACTAC TGAAACAGAA 
CTAAGAAAGA GACGGTCCAG TTCTTACCAT GTTTCCATGG ATTTGCTGGA AGATCCTACC 
ACAAGGCAAA GAGCAATGAG TCTAGCCAGT ATTTTGACCA ACACCATGGA AGAACTTGAA 
GAATCCAGAC AGAAATGCCC ACCCTGTTGG TATAAATTTG CTAACATGTG TTTGATTTGG 
GACTGCTGTA AACCATGGTT AAAGGTGAAA CATCTCGTCA ACCTGGTTGT AATGGACCCG 
TTTGTTGACC TGGCCATCAC CATCTGCATT GTCCTAAATA CACTGTTTAT GGCCATGGAG 
CACTATCCTA TGACAGAGCA GTTCAGCAGT GTGCTGTCAG TTGGGAACCT GGTCTTCACT 
GGGATCTTCA CAGCAGAAAT GTTCCTCAAG ATAATAGCCA TGGATCCATA TTATTACTTT 
CAAGAGGGCT GGAATATTTT TGATGGTTTA ATCGTAAGCC TTAGTTTAAT GGAACTTGGT 
TTGGCAAACG TTGAAGGATT GTCAGTTCTC CGATCATTTC GACTGCTCCG AGTTTTCAAA 
TTGGCAAAAT CCTGGCCCAC CCTGAATATG CTGATAAAGA TCATCGGCAA CTCAGTGGGG 
GCTCTGGGCA ACCTCACCTT GGTGTTGGCC ATCATCGTCT TCATTTTTGC CGTGGTTGGC 
ATGCAGCTCT TTGGTAAGAG CTACAAAGAA TGTGTCTGCA AGATCTCCAA TGATTGTGAG 
CTCCCACGGT GGCACATGCA TGACTTCTTC CACTCCTTCC TGATTGTATT CCGTGTGTTG 
TGTGGAGAGT GGATAGAAAC CATGTGGGAC TGTATGGAGG TCGCTGGCCA AACCATGTGC 
CTTACTGTCT TCATGATGGT CATGGTGATT GGAAACCTGG TGGTTCTGAA TCTCTTTCTG 
GCCTTGCTCT TGAGTTCATT CAGTTCTGAC AACCTTGCAG CCACTGACGA TGATAATGAA 
ATGAACAATC TCCAGATTGC TGTGGGAAGG ATGCAGAAAG GAATCGATTA TGTTAAAAGA 
AAACTACGTG AATTTATTCA GAAAGCCTTT GTTAGAAAGC AGAAAGCCTT AGATGAAATT 
AAACCTCTTG AAGATCTAAA TAACAAAAAA GACAGCTGTA TTTCCAACCA TACAACTATA 
GAAATAGGAA AAGATCTCAA TTATCTCAAA GATGGAAATG GAACTACCAG TGGCATAGGC 
AGCAGTGTGG AAAAATATGT TGTGGATGAA AGTGATTACA TGTCATTTAT AAACAATCCC 
AGCCTCACTG TGACTGTACC AATTGCTGTC GGGGAATCTG ACTTTGAAAA TTTAAATACT 
GAAGAATTTA GCAGCGAGTC AGATATGGAG GAAAGCAAAG AGAAGCTAAA TGCAACTAGT 
TCATCTGAAG GCAGCACAGT TGATATAGGA GCTCCCGCTG AGGGAGAGCA ACCTGAGGTA 
GAACCCGAGG AATCTCTTGA ACCTGAAGCC TGTTTCACGG AAGACTGTGT ACGGAAGTTC 
AAGTGTTGTC AGATAAGCAT AGAAGAAGGC AAAGGGAAGC TCTGGTGGAA CTTGAGAAAA 
ACTTGCTATA AGATAGTGGA GCACAACTGG TTTGAAACTT TCATTGTCTT CATGATTCTT 
CTCAGCAGTG GGGCACTGGC CTTTGAAGAT ATATATATTG AGCAGCGAAA AACCATTAAG 
ACCATGTTAG AATATGCCGA CAAAGTTTTC ACTTACATAT TCATTCTGGA AATGCTTCTC 
AAGTGGGTCG CATATGGTTT TCAAATGTAT TTCACCAATG CCTGGTGCTG GCTGGATTTC 
CTGATTGTTG ACGTCTCATT GGTTAGCTTG ACTGCAAACG CCTTGGGTTA CTCAGAACTT 
GGCGCCATCA AATCCCTCAG GACACTCAGA GCTCTGAGGC CATTGAGAGC CCTATCCCGG 
TTTGAAGGAA TGAGGGTTGT TGTCAATGCT CTTCTAGGAG CCATTCCATC CATAATGAAT 
GTGCTCCTGG TTTGTCTTAT CTTTTGGCTA ATATTCAGCA TCATGGGAGT AAATCTTTTC 
GCTGGCAAGT TTTACCATTG TATTAACTAC ACCACTGGAG AAATGTTTGA TGTAAGTGTG 
GTCAACAACT ACAGCGAATG TAAAGCCCTC ATAGAGAGCA ATCAAACTGC CAGGTGGAAA 
AATGTGAAAG TAAACTTTGA TAATGTAGGA CTTGGATATC TTTCTCTGCT TCAAGTAGCC 
ACATTTAAGG GATGGATGGA CATCATGTAT GCAGCTGTTG ATTCACGAAA TGTAGAATTA 
CAACCCAAGT ATGAAGACAA TCTGTACATG TACCTTTATT TTGTCATCTT TATTATTTTT 
GGTTCATTCT TTACCCTGAA TCTTTTCATT GGTGTCATCA TAGATAACTT CAACCAACAG 
AAAAAGAAGT TTGGAGGTCA AGACATTTTT ATGACAGAAG AGCAGAAGAA ATACTACAAT 
GCAATGAAAA AACTGGGTTC AAAGAAACCA CAAAAACCCA TACCTCGACC TGCTAATAAA 
TTCCAAGGAA TGGTCTTTGA TTTTGTAACC AAACAAGTCT TTGATATCAG TATCATGATC 
CTCATCTGCC TCAACATGGT CACCATGATG GTGGAAACTG ATGACCAGAG TCAGGAGATG 
ACAAACATTC TGTACTGGAT TAATCTGGTG TTTATTGTTC TGTTCACTGG AGAATGTGTG 
CTGAAATTAA TCTCCCTTCG TTATTACTAT TTCACCATTG GATGGAATAT TTTTGATTTT 
GTGGTGGTCA TTCTCTCTAT CGTAGGCATG TTTCTGGCTG AGCTGATAGA AAAATACTTT 
GTGTCCCCCA CCCTGTTCCG AGTGATCCGG CTCGCCAGGA TCGGTCGAAT CTTGCGTCTG 
ATCAAAGGGG CGAAGGGGAT CCGCACACTG CTCTTTGCTC TGATGATGTC CCTCCCTGCC 
TTGTTTAACA TCGGCCTCCT GCTCTTCCTG GTCATGTTCA TCTACGCCAT CTTTGGAATG 
TCCAACTTTG CCTATGTCAA GAGGGAAGTT GGAATTGATG ACATGTTCAA CTTTGAGACC 
TTTGGCAACA GCATGATCTG CTTGTTCCAA ATTACCACCT CGGCTGGCTG GGATGGATTG 
CTAGCACCTA TTCTTAATAG TGGACCTCCA GACTGTGATC CTGAAAAAGA TCACCCTGGA 
AGCTCAGTTA AGGGAGACTG TGGGAACCCA TCTGTGGGGA TTTTCTTTTT TGTCAGTTAC 
ATCATCATCT CCTTTCTGGT TGTGGTGAAC ATGTACATCG CTGTCATCCT GGAGAACTTC 
AGTGTTGCCA CTGAGGAGAG TGCAGAGCCC CTGAGCGAGG ATGACTTTGA GATGTTCTAT 
GAAGTCTGGG AGAAGTTTGA TCCTGATGCC ACCCAGTTTA TAGAGTTCTC CAAGCTCTCT 
GATTTTGCAG CTGCCCTGGA TCCTCCTCTT CTCATAGCGA AACCAAACAA AGTCCAGCTC 
ATTGCCATGG ACCTGCCCAT GGTCAGTGGG GACCGGATCC ACTGCCTCGA CATTTTATTT 
GCCTTTACAA AGCGTGTTTT GGGTGAGAGT GGAGAGATGG ATGCCCTTAG AATACAGATG 
GAGGAGCGAT TCATGGCATC GAACCCCTCC AAAGTCTCCT ATGAACCCAT CACAACCACT 
CTGAAACGCA AACAAGAGGA GGTGTCTGCT ATCGTTATCC AGAGGGCTTA TAGACGCTAC 
CTCTTGAAGC AGAAAGTTAA AAAGGTTTCA TGTATCTATA AGAAAGACAA AGGCAAAGAA 
GGGGATGGAA CACCTATCAA AGAAGATATC CTCATTGACA AACTGAATGA GAATTCGACG 
CCAGAGAAAA CCGATGTGAC ACCTTCCACC ACTTCTCCAC CTTCGTATGA TAGTGTGACC 
AAACCAGAAA AAGAAAAATT TGAAAAAGAC AAATCAGAAA AGGAAGACAA AGGGAAAGAT 
ATCAGGGAAA GTAAAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC478773 ( XM_535939.6 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_535939.2 Canis lupus familiaris sodium channel protein type 2 subunit alpha (LOC478773), transcript variant X1, mRNA.

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CloneID	OCe31042
Clone ID Related Accession (Same CDS sequence)	XM_535939.5 , XM_014110824.2 , XM_022414740.1 , XM_014110825.2 , XM_014110824.1 , XM_014110825.1 , XM_535939.6
Accession Version	XM_535939.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	sodium channel protein type 2 subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006618.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_535939.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGT CAGTGCTGGT ACCACCAGGA CCTGACAGCT TCCGCTTCTT TACCAGGGAA 
TCCCTTGCTG CTATTGAACA ACGCATTGCA GAAGAGAAAG CTAAGAGACC CAAACAGGAG 
CGTAAGGATG AGGATGATGA AAATGGCCCA AAGCCAAACA GTGACTTGGA AGCAGGGAAA 
TCTCTTCCAT TTATTTATGG AGACATTCCT CCAGAGATGG TGTCAGAGCC CTTGGAGGAC 
CTGGACCCGT ACTATATCAA TAAGAAAACA TTTATAGTAT TGAATAAAGG GAAAGCAATC 
TCTCGATTCA GTGCCACCCC TGCCCTGTAC ATTTTAACTC CCTTCAACCC TATTAGAAAA 
TTAGCTATTA AGATTTTGGT ACATTCTTTA TTCAATGTGC TCATTATGTG CACTATTCTC 
ACCAACTGTG TATTTATGAC CATGAGTAAC CCTCCAGACT GGACAAAGAA TGTGGAGTAC 
ACCTTTACAG GAATTTATAC ATTTGAATCA CTTATAAAAA TACTTGCAAG GGGCTTTTGT 
TTAGAAGATT TCACATTTCT CCGGGATCCA TGGAATTGGT TGGATTTCAC AGTCATTACT 
TTTGCATACG TGACAGAGTT TGTGGATCTG GGCAATGTCT CAGCGCTGAG AACATTCAGA 
GTTCTCCGAG CACTGAAAAC AATTTCAGTC ATTCCAGGCC TGAAGACCAT TGTGGGGGCC 
CTGATCCAGT CGGTGAAGAA GCTCTCTGAT GTAATGATTC TGACTGTGTT CTGTCTGAGT 
GTGTTTGCAC TCATAGGACT GCAGCTGTTT ATGGGCAACC TGCGGAATAA ATGCTTGCAA 
TGGCCCCCAG ATAATTCTTC CTTTGAAATA AACATCACTT CCTTCTTTAA TAATTCATTG 
GATGGGAACG GTACCATGTT CAATAGGACA GTGAGCATGT TTAACTGGGA TGAATATATT 
GAGGATAAAA GTCACTTTTA TTTTTTGGAA GGGCAAAATG ATGCTCTGCT TTGTGGCAAC 
AGCTCAGATG CAGGCCAGTG TCCGGAAGGA TATATCTGTG TGAAGGCTGG TAGAAACCCC 
AACTATGGCT ATACAAGCTT TGATACCTTT AGTTGGGCCT TCTTGTCATT ATTTCGTCTC 
ATGACTCAAG ACTTCTGGGA AAACCTTTAC CAACTGACGC TACGTGCTGC TGGGAAAACA 
TATATGATAT TTTTTGTGCT GGTCATTTTC TTGGGCTCAT TCTACCTTAT AAATTTAATC 
CTGGCTGTGG TGGCTATGGC CTACGAGGAA CAGAATCAGG CAACTCTTGA GGAAGCTGAA 
CAGAAAGAAG CTGAATTTCA GCAGATGCTC GAACAACTAA AAAAACAACA AGAAGAAGCT 
CAGGCAGCAG CTGCAGCCGC ATCTGCAGAA TCAAGAGAGT TCAGTGGTGC TGGTGGGATA 
GGAGTTTTCT CAGAGAGTTC TTCAGTAGCA TCAAAGTTGA GCTCCAAGAG TGAAAAAGAG 
CTGAAAAACA GAAGGAAGAA AAAGAAGCAG AAAGAGCAGT CTGGAGAAGA AGAGAAGGAA 
GATGGGGTTC GCAAATCCGA ATCCGAAGAC AGTATAAGAA GAAAAGGTTT CCGTTTTTCC 
TTAGAAGGAA GCAGGCTGAC ATATGAAAAA AGGTTCTCTT CTCCACATCA GTCTTTATTG 
AGCATCCGTG GCTCCCTTTT CTCTCCAAGA CGCAACAGTA GGGCGAGCCT TTTCAGTTTC 
AGAGGCCGAA CAAAGGATAT TGGCTCTGAG AACGACTTTG CTGATGATGA GCACAGCACC 
TTTGAAGACA ATGACAGCCG GAGAGACTCT CTGTTTGTGC CACACAGACA TGGAGAGCGG 
CGTCATAGCA ATGTCAGCCA GGCCAGCCGG GCCTCCAGGG TGATCCCCAT CCTGCCGGTG 
AATGGAAAGA TGCACAGTGC AGTGGATTGC AACGGGGTCG TCTCGCTGGT TGGGGGCCCT 
TCTGCCCTCA CGTCTCCAGC TGGACAGCTC CTTCCGGAGG GCACGACTAC TGAAACAGAA 
CTAAGAAAGA GACGGTCCAG TTCTTACCAT GTTTCCATGG ATTTGCTGGA AGATCCTACC 
ACAAGGCAAA GAGCAATGAG TCTAGCCAGT ATTTTGACCA ACACCATGGA AGAACTTGAA 
GAATCCAGAC AGAAATGCCC ACCCTGTTGG TATAAATTTG CTAACATGTG TTTGATTTGG 
GACTGCTGTA AACCATGGTT AAAGGTGAAA CATCTCGTCA ACCTGGTTGT AATGGACCCG 
TTTGTTGACC TGGCCATCAC CATCTGCATT GTCCTAAATA CACTGTTTAT GGCCATGGAG 
CACTATCCTA TGACAGAGCA GTTCAGCAGT GTGCTGTCAG TTGGGAACCT GGTCTTCACT 
GGGATCTTCA CAGCAGAAAT GTTCCTCAAG ATAATAGCCA TGGATCCATA TTATTACTTT 
CAAGAGGGCT GGAATATTTT TGATGGTTTA ATCGTAAGCC TTAGTTTAAT GGAACTTGGT 
TTGGCAAACG TTGAAGGATT GTCAGTTCTC CGATCATTTC GACTGCTCCG AGTTTTCAAA 
TTGGCAAAAT CCTGGCCCAC CCTGAATATG CTGATAAAGA TCATCGGCAA CTCAGTGGGG 
GCTCTGGGCA ACCTCACCTT GGTGTTGGCC ATCATCGTCT TCATTTTTGC CGTGGTTGGC 
ATGCAGCTCT TTGGTAAGAG CTACAAAGAA TGTGTCTGCA AGATCTCCAA TGATTGTGAG 
CTCCCACGGT GGCACATGCA TGACTTCTTC CACTCCTTCC TGATTGTATT CCGTGTGTTG 
TGTGGAGAGT GGATAGAAAC CATGTGGGAC TGTATGGAGG TCGCTGGCCA AACCATGTGC 
CTTACTGTCT TCATGATGGT CATGGTGATT GGAAACCTGG TGGTTCTGAA TCTCTTTCTG 
GCCTTGCTCT TGAGTTCATT CAGTTCTGAC AACCTTGCAG CCACTGACGA TGATAATGAA 
ATGAACAATC TCCAGATTGC TGTGGGAAGG ATGCAGAAAG GAATCGATTA TGTTAAAAGA 
AAACTACGTG AATTTATTCA GAAAGCCTTT GTTAGAAAGC AGAAAGCCTT AGATGAAATT 
AAACCTCTTG AAGATCTAAA TAACAAAAAA GACAGCTGTA TTTCCAACCA TACAACTATA 
GAAATAGGAA AAGATCTCAA TTATCTCAAA GATGGAAATG GAACTACCAG TGGCATAGGC 
AGCAGTGTGG AAAAATATGT TGTGGATGAA AGTGATTACA TGTCATTTAT AAACAATCCC 
AGCCTCACTG TGACTGTACC AATTGCTGTC GGGGAATCTG ACTTTGAAAA TTTAAATACT 
GAAGAATTTA GCAGCGAGTC AGATATGGAG GAAAGCAAAG AGAAGCTAAA TGCAACTAGT 
TCATCTGAAG GCAGCACAGT TGATATAGGA GCTCCCGCTG AGGGAGAGCA ACCTGAGGTA 
GAACCCGAGG AATCTCTTGA ACCTGAAGCC TGTTTCACGG AAGACTGTGT ACGGAAGTTC 
AAGTGTTGTC AGATAAGCAT AGAAGAAGGC AAAGGGAAGC TCTGGTGGAA CTTGAGAAAA 
ACTTGCTATA AGATAGTGGA GCACAACTGG TTTGAAACTT TCATTGTCTT CATGATTCTT 
CTCAGCAGTG GGGCACTGGC CTTTGAAGAT ATATATATTG AGCAGCGAAA AACCATTAAG 
ACCATGTTAG AATATGCCGA CAAAGTTTTC ACTTACATAT TCATTCTGGA AATGCTTCTC 
AAGTGGGTCG CATATGGTTT TCAAATGTAT TTCACCAATG CCTGGTGCTG GCTGGATTTC 
CTGATTGTTG ACGTCTCATT GGTTAGCTTG ACTGCAAACG CCTTGGGTTA CTCAGAACTT 
GGCGCCATCA AATCCCTCAG GACACTCAGA GCTCTGAGGC CATTGAGAGC CCTATCCCGG 
TTTGAAGGAA TGAGGGTTGT TGTCAATGCT CTTCTAGGAG CCATTCCATC CATAATGAAT 
GTGCTCCTGG TTTGTCTTAT CTTTTGGCTA ATATTCAGCA TCATGGGAGT AAATCTTTTC 
GCTGGCAAGT TTTACCATTG TATTAACTAC ACCACTGGAG AAATGTTTGA TGTAAGTGTG 
GTCAACAACT ACAGCGAATG TAAAGCCCTC ATAGAGAGCA ATCAAACTGC CAGGTGGAAA 
AATGTGAAAG TAAACTTTGA TAATGTAGGA CTTGGATATC TTTCTCTGCT TCAAGTAGCC 
ACATTTAAGG GATGGATGGA CATCATGTAT GCAGCTGTTG ATTCACGAAA TGTAGAATTA 
CAACCCAAGT ATGAAGACAA TCTGTACATG TACCTTTATT TTGTCATCTT TATTATTTTT 
GGTTCATTCT TTACCCTGAA TCTTTTCATT GGTGTCATCA TAGATAACTT CAACCAACAG 
AAAAAGAAGT TTGGAGGTCA AGACATTTTT ATGACAGAAG AGCAGAAGAA ATACTACAAT 
GCAATGAAAA AACTGGGTTC AAAGAAACCA CAAAAACCCA TACCTCGACC TGCTAATAAA 
TTCCAAGGAA TGGTCTTTGA TTTTGTAACC AAACAAGTCT TTGATATCAG TATCATGATC 
CTCATCTGCC TCAACATGGT CACCATGATG GTGGAAACTG ATGACCAGAG TCAGGAGATG 
ACAAACATTC TGTACTGGAT TAATCTGGTG TTTATTGTTC TGTTCACTGG AGAATGTGTG 
CTGAAATTAA TCTCCCTTCG TTATTACTAT TTCACCATTG GATGGAATAT TTTTGATTTT 
GTGGTGGTCA TTCTCTCTAT CGTAGGCATG TTTCTGGCTG AGCTGATAGA AAAATACTTT 
GTGTCCCCCA CCCTGTTCCG AGTGATCCGG CTCGCCAGGA TCGGTCGAAT CTTGCGTCTG 
ATCAAAGGGG CGAAGGGGAT CCGCACACTG CTCTTTGCTC TGATGATGTC CCTCCCTGCC 
TTGTTTAACA TCGGCCTCCT GCTCTTCCTG GTCATGTTCA TCTACGCCAT CTTTGGAATG 
TCCAACTTTG CCTATGTCAA GAGGGAAGTT GGAATTGATG ACATGTTCAA CTTTGAGACC 
TTTGGCAACA GCATGATCTG CTTGTTCCAA ATTACCACCT CGGCTGGCTG GGATGGATTG 
CTAGCACCTA TTCTTAATAG TGGACCTCCA GACTGTGATC CTGAAAAAGA TCACCCTGGA 
AGCTCAGTTA AGGGAGACTG TGGGAACCCA TCTGTGGGGA TTTTCTTTTT TGTCAGTTAC 
ATCATCATCT CCTTTCTGGT TGTGGTGAAC ATGTACATCG CTGTCATCCT GGAGAACTTC 
AGTGTTGCCA CTGAGGAGAG TGCAGAGCCC CTGAGCGAGG ATGACTTTGA GATGTTCTAT 
GAAGTCTGGG AGAAGTTTGA TCCTGATGCC ACCCAGTTTA TAGAGTTCTC CAAGCTCTCT 
GATTTTGCAG CTGCCCTGGA TCCTCCTCTT CTCATAGCGA AACCAAACAA AGTCCAGCTC 
ATTGCCATGG ACCTGCCCAT GGTCAGTGGG GACCGGATCC ACTGCCTCGA CATTTTATTT 
GCCTTTACAA AGCGTGTTTT GGGTGAGAGT GGAGAGATGG ATGCCCTTAG AATACAGATG 
GAGGAGCGAT TCATGGCATC GAACCCCTCC AAAGTCTCCT ATGAACCCAT CACAACCACT 
CTGAAACGCA AACAAGAGGA GGTGTCTGCT ATCGTTATCC AGAGGGCTTA TAGACGCTAC 
CTCTTGAAGC AGAAAGTTAA AAAGGTTTCA TGTATCTATA AGAAAGACAA AGGCAAAGAA 
GGGGATGGAA CACCTATCAA AGAAGATATC CTCATTGACA AACTGAATGA GAATTCGACG 
CCAGAGAAAA CCGATGTGAC ACCTTCCACC ACTTCTCCAC CTTCGTATGA TAGTGTGACC 
AAACCAGAAA AAGAAAAATT TGAAAAAGAC AAATCAGAAA AGGAAGACAA AGGGAAAGAT 
ATCAGGGAAA GTAAAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_535939.2
CDS	187..6207
Translation	MAQSVLVPPGPDSFRFFTRESLAAIEQRIAEEKAKRPKQERKDEDDENGPKPNSDLEAGKSLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAISRFSATPALYILTPFNPIRKLAIKILVHSLFNVLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKILARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCLQWPPDNSSFEINITSFFNNSLDGNGTMFNRTVSMFNWDEYIEDKSHFYFLEGQNDALLCGNSSDAGQCPEGYICVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMLEQLKKQQEEAQAAAAAASAESREFSGAGGIGVFSESSSVASKLSSKSEKELKNRRKKKKQKEQSGEEEKEDGVRKSESEDSIRRKGFRFSLEGSRLTYEKRFSSPHQSLLSIRGSLFSPRRNSRASLFSFRGRTKDIGSENDFADDEHSTFEDNDSRRDSLFVPHRHGERRHSNVSQASRASRVIPILPVNGKMHSAVDCNGVVSLVGGPSALTSPAGQLLPEGTTTETELRKRRSSSYHVSMDLLEDPTTRQRAMSLASILTNTMEELEESRQKCPPCWYKFANMCLIWDCCKPWLKVKHLVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTEQFSSVLSVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGLIVSLSLMELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKISNDCELPRWHMHDFFHSFLIVFRVLCGEWIETMWDCMEVAGQTMCLTVFMMVMVIGNLVVLNLFLALLLSSFSSDNLAATDDDNEMNNLQIAVGRMQKGIDYVKRKLREFIQKAFVRKQKALDEIKPLEDLNNKKDSCISNHTTIEIGKDLNYLKDGNGTTSGIGSSVEKYVVDESDYMSFINNPSLTVTVPIAVGESDFENLNTEEFSSESDMEESKEKLNATSSSEGSTVDIGAPAEGEQPEVEPEESLEPEACFTEDCVRKFKCCQISIEEGKGKLWWNLRKTCYKIVEHNWFETFIVFMILLSSGALAFEDIYIEQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGFQMYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINYTTGEMFDVSVVNNYSECKALIESNQTARWKNVKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEDNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPANKFQGMVFDFVTKQVFDISIMILICLNMVTMMVETDDQSQEMTNILYWINLVFIVLFTGECVLKLISLRYYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSGPPDCDPEKDHPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFIEFSKLSDFAAALDPPLLIAKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYEPITTTLKRKQEEVSAIVIQRAYRRYLLKQKVKKVSCIYKKDKGKEGDGTPIKEDILIDKLNENSTPEKTDVTPSTTSPPSYDSVTKPEKEKFEKDKSEKEDKGKDIRESKK

Target ORF information:

RefSeq Version	XM_535939.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris sodium channel protein type 2 subunit alpha (LOC478773), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_535939.6

ORF Insert Sequence:

ATGGCACAGT CAGTGCTGGT ACCACCAGGA CCTGACAGCT TCCGCTTCTT TACCAGGGAA 
TCCCTTGCTG CTATTGAACA ACGCATTGCA GAAGAGAAAG CTAAGAGACC CAAACAGGAG 
CGTAAGGATG AGGATGATGA AAATGGCCCA AAGCCAAACA GTGACTTGGA AGCAGGGAAA 
TCTCTTCCAT TTATTTATGG AGACATTCCT CCAGAGATGG TGTCAGAGCC CTTGGAGGAC 
CTGGACCCGT ACTATATCAA TAAGAAAACA TTTATAGTAT TGAATAAAGG GAAAGCAATC 
TCTCGATTCA GTGCCACCCC TGCCCTGTAC ATTTTAACTC CCTTCAACCC TATTAGAAAA 
TTAGCTATTA AGATTTTGGT ACATTCTTTA TTCAATGTGC TCATTATGTG CACTATTCTC 
ACCAACTGTG TATTTATGAC CATGAGTAAC CCTCCAGACT GGACAAAGAA TGTGGAGTAC 
ACCTTTACAG GAATTTATAC ATTTGAATCA CTTATAAAAA TACTTGCAAG GGGCTTTTGT 
TTAGAAGATT TCACATTTCT CCGGGATCCA TGGAATTGGT TGGATTTCAC AGTCATTACT 
TTTGCATACG TGACAGAGTT TGTGGATCTG GGCAATGTCT CAGCGCTGAG AACATTCAGA 
GTTCTCCGAG CACTGAAAAC AATTTCAGTC ATTCCAGGCC TGAAGACCAT TGTGGGGGCC 
CTGATCCAGT CGGTGAAGAA GCTCTCTGAT GTAATGATTC TGACTGTGTT CTGTCTGAGT 
GTGTTTGCAC TCATAGGACT GCAGCTGTTT ATGGGCAACC TGCGGAATAA ATGCTTGCAA 
TGGCCCCCAG ATAATTCTTC CTTTGAAATA AACATCACTT CCTTCTTTAA TAATTCATTG 
GATGGGAACG GTACCATGTT CAATAGGACA GTGAGCATGT TTAACTGGGA TGAATATATT 
GAGGATAAAA GTCACTTTTA TTTTTTGGAA GGGCAAAATG ATGCTCTGCT TTGTGGCAAC 
AGCTCAGATG CAGGCCAGTG TCCGGAAGGA TATATCTGTG TGAAGGCTGG TAGAAACCCC 
AACTATGGCT ATACAAGCTT TGATACCTTT AGTTGGGCCT TCTTGTCATT ATTTCGTCTC 
ATGACTCAAG ACTTCTGGGA AAACCTTTAC CAACTGACGC TACGTGCTGC TGGGAAAACA 
TATATGATAT TTTTTGTGCT GGTCATTTTC TTGGGCTCAT TCTACCTTAT AAATTTAATC 
CTGGCTGTGG TGGCTATGGC CTACGAGGAA CAGAATCAGG CAACTCTTGA GGAAGCTGAA 
CAGAAAGAAG CTGAATTTCA GCAGATGCTC GAACAACTAA AAAAACAACA AGAAGAAGCT 
CAGGCAGCAG CTGCAGCCGC ATCTGCAGAA TCAAGAGAGT TCAGTGGTGC TGGTGGGATA 
GGAGTTTTCT CAGAGAGTTC TTCAGTAGCA TCAAAGTTGA GCTCCAAGAG TGAAAAAGAG 
CTGAAAAACA GAAGGAAGAA AAAGAAGCAG AAAGAGCAGT CTGGAGAAGA AGAGAAGGAA 
GATGGGGTTC GCAAATCCGA ATCCGAAGAC AGTATAAGAA GAAAAGGTTT CCGTTTTTCC 
TTAGAAGGAA GCAGGCTGAC ATATGAAAAA AGGTTCTCTT CTCCACATCA GTCTTTATTG 
AGCATCCGTG GCTCCCTTTT CTCTCCAAGA CGCAACAGTA GGGCGAGCCT TTTCAGTTTC 
AGAGGCCGAA CAAAGGATAT TGGCTCTGAG AACGACTTTG CTGATGATGA GCACAGCACC 
TTTGAAGACA ATGACAGCCG GAGAGACTCT CTGTTTGTGC CACACAGACA TGGAGAGCGG 
CGTCATAGCA ATGTCAGCCA GGCCAGCCGG GCCTCCAGGG TGATCCCCAT CCTGCCGGTG 
AATGGAAAGA TGCACAGTGC AGTGGATTGC AACGGGGTCG TCTCGCTGGT TGGGGGCCCT 
TCTGCCCTCA CGTCTCCAGC TGGACAGCTC CTTCCGGAGG GCACGACTAC TGAAACAGAA 
CTAAGAAAGA GACGGTCCAG TTCTTACCAT GTTTCCATGG ATTTGCTGGA AGATCCTACC 
ACAAGGCAAA GAGCAATGAG TCTAGCCAGT ATTTTGACCA ACACCATGGA AGAACTTGAA 
GAATCCAGAC AGAAATGCCC ACCCTGTTGG TATAAATTTG CTAACATGTG TTTGATTTGG 
GACTGCTGTA AACCATGGTT AAAGGTGAAA CATCTCGTCA ACCTGGTTGT AATGGACCCG 
TTTGTTGACC TGGCCATCAC CATCTGCATT GTCCTAAATA CACTGTTTAT GGCCATGGAG 
CACTATCCTA TGACAGAGCA GTTCAGCAGT GTGCTGTCAG TTGGGAACCT GGTCTTCACT 
GGGATCTTCA CAGCAGAAAT GTTCCTCAAG ATAATAGCCA TGGATCCATA TTATTACTTT 
CAAGAGGGCT GGAATATTTT TGATGGTTTA ATCGTAAGCC TTAGTTTAAT GGAACTTGGT 
TTGGCAAACG TTGAAGGATT GTCAGTTCTC CGATCATTTC GACTGCTCCG AGTTTTCAAA 
TTGGCAAAAT CCTGGCCCAC CCTGAATATG CTGATAAAGA TCATCGGCAA CTCAGTGGGG 
GCTCTGGGCA ACCTCACCTT GGTGTTGGCC ATCATCGTCT TCATTTTTGC CGTGGTTGGC 
ATGCAGCTCT TTGGTAAGAG CTACAAAGAA TGTGTCTGCA AGATCTCCAA TGATTGTGAG 
CTCCCACGGT GGCACATGCA TGACTTCTTC CACTCCTTCC TGATTGTATT CCGTGTGTTG 
TGTGGAGAGT GGATAGAAAC CATGTGGGAC TGTATGGAGG TCGCTGGCCA AACCATGTGC 
CTTACTGTCT TCATGATGGT CATGGTGATT GGAAACCTGG TGGTTCTGAA TCTCTTTCTG 
GCCTTGCTCT TGAGTTCATT CAGTTCTGAC AACCTTGCAG CCACTGACGA TGATAATGAA 
ATGAACAATC TCCAGATTGC TGTGGGAAGG ATGCAGAAAG GAATCGATTA TGTTAAAAGA 
AAACTACGTG AATTTATTCA GAAAGCCTTT GTTAGAAAGC AGAAAGCCTT AGATGAAATT 
AAACCTCTTG AAGATCTAAA TAACAAAAAA GACAGCTGTA TTTCCAACCA TACAACTATA 
GAAATAGGAA AAGATCTCAA TTATCTCAAA GATGGAAATG GAACTACCAG TGGCATAGGC 
AGCAGTGTGG AAAAATATGT TGTGGATGAA AGTGATTACA TGTCATTTAT AAACAATCCC 
AGCCTCACTG TGACTGTACC AATTGCTGTC GGGGAATCTG ACTTTGAAAA TTTAAATACT 
GAAGAATTTA GCAGCGAGTC AGATATGGAG GAAAGCAAAG AGAAGCTAAA TGCAACTAGT 
TCATCTGAAG GCAGCACAGT TGATATAGGA GCTCCCGCTG AGGGAGAGCA ACCTGAGGTA 
GAACCCGAGG AATCTCTTGA ACCTGAAGCC TGTTTCACGG AAGACTGTGT ACGGAAGTTC 
AAGTGTTGTC AGATAAGCAT AGAAGAAGGC AAAGGGAAGC TCTGGTGGAA CTTGAGAAAA 
ACTTGCTATA AGATAGTGGA GCACAACTGG TTTGAAACTT TCATTGTCTT CATGATTCTT 
CTCAGCAGTG GGGCACTGGC CTTTGAAGAT ATATATATTG AGCAGCGAAA AACCATTAAG 
ACCATGTTAG AATATGCCGA CAAAGTTTTC ACTTACATAT TCATTCTGGA AATGCTTCTC 
AAGTGGGTCG CATATGGTTT TCAAATGTAT TTCACCAATG CCTGGTGCTG GCTGGATTTC 
CTGATTGTTG ACGTCTCATT GGTTAGCTTG ACTGCAAACG CCTTGGGTTA CTCAGAACTT 
GGCGCCATCA AATCCCTCAG GACACTCAGA GCTCTGAGGC CATTGAGAGC CCTATCCCGG 
TTTGAAGGAA TGAGGGTTGT TGTCAATGCT CTTCTAGGAG CCATTCCATC CATAATGAAT 
GTGCTCCTGG TTTGTCTTAT CTTTTGGCTA ATATTCAGCA TCATGGGAGT AAATCTTTTC 
GCTGGCAAGT TTTACCATTG TATTAACTAC ACCACTGGAG AAATGTTTGA TGTAAGTGTG 
GTCAACAACT ACAGCGAATG TAAAGCCCTC ATAGAGAGCA ATCAAACTGC CAGGTGGAAA 
AATGTGAAAG TAAACTTTGA TAATGTAGGA CTTGGATATC TTTCTCTGCT TCAAGTAGCC 
ACATTTAAGG GATGGATGGA CATCATGTAT GCAGCTGTTG ATTCACGAAA TGTAGAATTA 
CAACCCAAGT ATGAAGACAA TCTGTACATG TACCTTTATT TTGTCATCTT TATTATTTTT 
GGTTCATTCT TTACCCTGAA TCTTTTCATT GGTGTCATCA TAGATAACTT CAACCAACAG 
AAAAAGAAGT TTGGAGGTCA AGACATTTTT ATGACAGAAG AGCAGAAGAA ATACTACAAT 
GCAATGAAAA AACTGGGTTC AAAGAAACCA CAAAAACCCA TACCTCGACC TGCTAATAAA 
TTCCAAGGAA TGGTCTTTGA TTTTGTAACC AAACAAGTCT TTGATATCAG TATCATGATC 
CTCATCTGCC TCAACATGGT CACCATGATG GTGGAAACTG ATGACCAGAG TCAGGAGATG 
ACAAACATTC TGTACTGGAT TAATCTGGTG TTTATTGTTC TGTTCACTGG AGAATGTGTG 
CTGAAATTAA TCTCCCTTCG TTATTACTAT TTCACCATTG GATGGAATAT TTTTGATTTT 
GTGGTGGTCA TTCTCTCTAT CGTAGGCATG TTTCTGGCTG AGCTGATAGA AAAATACTTT 
GTGTCCCCCA CCCTGTTCCG AGTGATCCGG CTCGCCAGGA TCGGTCGAAT CTTGCGTCTG 
ATCAAAGGGG CGAAGGGGAT CCGCACACTG CTCTTTGCTC TGATGATGTC CCTCCCTGCC 
TTGTTTAACA TCGGCCTCCT GCTCTTCCTG GTCATGTTCA TCTACGCCAT CTTTGGAATG 
TCCAACTTTG CCTATGTCAA GAGGGAAGTT GGAATTGATG ACATGTTCAA CTTTGAGACC 
TTTGGCAACA GCATGATCTG CTTGTTCCAA ATTACCACCT CGGCTGGCTG GGATGGATTG 
CTAGCACCTA TTCTTAATAG TGGACCTCCA GACTGTGATC CTGAAAAAGA TCACCCTGGA 
AGCTCAGTTA AGGGAGACTG TGGGAACCCA TCTGTGGGGA TTTTCTTTTT TGTCAGTTAC 
ATCATCATCT CCTTTCTGGT TGTGGTGAAC ATGTACATCG CTGTCATCCT GGAGAACTTC 
AGTGTTGCCA CTGAGGAGAG TGCAGAGCCC CTGAGCGAGG ATGACTTTGA GATGTTCTAT 
GAAGTCTGGG AGAAGTTTGA TCCTGATGCC ACCCAGTTTA TAGAGTTCTC CAAGCTCTCT 
GATTTTGCAG CTGCCCTGGA TCCTCCTCTT CTCATAGCGA AACCAAACAA AGTCCAGCTC 
ATTGCCATGG ACCTGCCCAT GGTCAGTGGG GACCGGATCC ACTGCCTCGA CATTTTATTT 
GCCTTTACAA AGCGTGTTTT GGGTGAGAGT GGAGAGATGG ATGCCCTTAG AATACAGATG 
GAGGAGCGAT TCATGGCATC GAACCCCTCC AAAGTCTCCT ATGAACCCAT CACAACCACT 
CTGAAACGCA AACAAGAGGA GGTGTCTGCT ATCGTTATCC AGAGGGCTTA TAGACGCTAC 
CTCTTGAAGC AGAAAGTTAA AAAGGTTTCA TGTATCTATA AGAAAGACAA AGGCAAAGAA 
GGGGATGGAA CACCTATCAA AGAAGATATC CTCATTGACA AACTGAATGA GAATTCGACG 
CCAGAGAAAA CCGATGTGAC ACCTTCCACC ACTTCTCCAC CTTCGTATGA TAGTGTGACC 
AAACCAGAAA AAGAAAAATT TGAAAAAGAC AAATCAGAAA AGGAAGACAA AGGGAAAGAT 
ATCAGGGAAA GTAAAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	LOC478773
Entrez Gene ID	478773
Full Name	sodium channel protein type 2 subunit alpha
Synonyms	SCN2A
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Homo sapiens (human)	SCN2A	NP_001035233.1
Gallus gallus (chicken)	SCN2A	XP_004942844.1
	SCN2A	XP_001099916.1
Canis lupus familiaris (dog)	SCN2A	XP_535939.2
Rattus norvegicus (Norway rat)	Scn2a1	NP_036779.1
Drosophila melanogaster (fruit fly)	para	NP_001188624.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004707	XP_554647.3
Xenopus tropicalis (tropical clawed frog)	scn3a	XP_002939317.2
Mus musculus (house mouse)	Scn2a1	NP_001092768.1
Bos taurus (cattle)	SCN2A	NP_001137581.1
Pan troglodytes (chimpanzee)	SCN2A	XP_001153227.1
Danio rerio (zebrafish)	scn1lab	NP_001038360.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

LOC478773 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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