TRIO cDNA ORF clone, Canis lupus familiaris(dog)

The following TRIO gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRIO cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe284506 XM_022414357.1
Latest version!
Canis lupus familiaris trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe284507 XM_022414358.1
Latest version!
Canis lupus familiaris trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe284504 XM_022414355.1
Latest version!
Canis lupus familiaris trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OCe284505 XM_022414356.1
Latest version!
Canis lupus familiaris trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OCe44775 XM_014110313.1
Latest version!
Canis lupus familiaris trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OCe44776 XM_014110314.1
Latest version!
Canis lupus familiaris trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe284506
    Clone ID Related Accession (Same CDS sequence) XM_022414357.1
    Accession Version XM_022414357.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5088bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product triple functional domain protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAAAGCTA TGGATGTTTT GCCAATTTTA AAGGAAAAAG TTGCATACCT TTCAGGTGGC 
    AGAGATAAAC GTGGAGGTCC CATTTTAACA TTTCCTGCTC GCAGCAATCA TGATAGAATA
    CGACAGGAGG ACCTCAGGAG ACTCATTTCC TATCTAGCCT GTATCCCTAG TGAAGAGGTC
    TGCAAGCGAG GATTCACGGT GATCGTGGAC ATGCGTGGGT CCAAGTGGGA CTCCATTAAG
    CCTCTGCTGA AGATATTGCA AGAGTCCTTT CCCTGCTGCA TTCATGTTGC ACTGATAATC
    AAGCCAGACA ACTTTTGGCA GAAACAGAGG ACTAATTTTG GCAGTTCTAA GTTTGAATTT
    GAGACAAATA TGGTCTCTTT AGAAGGCCTT ACCAAAGTAG TTGATCCTTC TCAGCTAACT
    CCCGAGTTTG ATGGCTGCTT GGAATACAAC CATGAAGAAT GGATTGAAAT CAGAGTTGCT
    TTTGAAGACT ACATTAGCAA TGCAACCCAC ATGCTCTCTC GGCTGGAGGA ACTTCAGGAC
    ATCCTAGCCA AGAAAGAATT GCCTCAGGAT TTAGAGGGGG CTCGGAATAT GATTGAGGAA
    CATTCCCAGC TAAAGAAGAA GGTGATTAAG GCCCCTATAG AAGACTTAGA TTTAGAGGGA
    CAGAAGCTGC TCCAGAGGAT TCAGAGCAGT GACAGCTTTC CCAAAAAGAA CTCTGGGTCA
    GGCAATGCGG ACCTGCAGAG CCTCCTACCC AAGGTGTCCG CCATGTTGGA CAGGCTGCAC
    TCAACACGGC AGCATCTGCA TCAGATGTGG CATGTACGAA AACTGAAGCT GGACCAGTGC
    TTTCAGCTAA GGCTGTTTGA ACAGGACGCT GAAAAGATGT TTGACTGGAT CACACATAAC
    AAGGGCCTGT TTCTGAACAG CTACACTGAA ATTGGGACCA GCCATCCTCA TGCAATGGAA
    CTCCAGACAC AGCACAATCA CTTTGCCATG AACTGTATGA ACGTGTATGT TAATATAAAT
    CGCATAATGT CAGTGGCAAA TCGCTTGGTA GAGTCTGGCC ACTACGCCTC TCAGCAGATT
    AAGCAGATTG CGAATCAGCT GGAGCAGGAG TGGAAGGCAT TTGCGGCAGC CCTGGATGAG
    CGGAGTACCT TGCTGGACAT GTCCTCCATT TTCCACCAGA AGGCTGAAAA GTATATGAGC
    AACGTGGATT CGTGGTGTAA AGCCTGTGGT GAGGTAGACC TTCCCTCGGA GCTGCAGGAT
    CTGGAGGATG CCATTCACCA CCACCAGGGA ATATATGAGC ACATCACCCT TGCTTATTCC
    GAGGTGAGCC AGGATGGGAA GTCACTCCTC GACAAGCTCC AGCGGCCTTT GACTCCTGGC
    AGCTCCGACT CCCTCACGGC CTCTGCCAAC TACTCCAAGG CTGTGCACCA TGTCCTTGAT
    GTCATCCATG AGGTGCTGCA CCACCAGCGA CAGCTTGAGA ACATCTGGCA GCACCGCAAG
    GTCCGGCTCC ACCAGCGGCT GCAGCTGTGT GTGTTCCAGC AAGACGTTCA GCAGGTGCTG
    GACTGGATTG AGAACCATGG AGAAGCATTT CTGAGCAAAC ATACAGGTGT GGGGAAATCT
    CTTCACCGGG CCAGAGCTTT GCAGAAACGT CATGAAGATT TTGAAGAAGT AGCACAGAAC
    ACATATACCA ACGCAGATAA ATTACTAGAA GCAGCAGAAC AGCTGGCTCA GACCGGGGAA
    TGTGACCCAG AAGAGATTTA TCAGGCTGCG CATCAGCTTG AAGACCGGAT ACAAGATTTT
    GTTCGGCGCG TAGAGCAGCG CAAGATCCTG CTGGACATGT CAGTGTCGTT TCATAGCCAC
    GTCAAGGAGC TGTGGACATG GCTGGAGGAG CTGCAGAAGG AGCTGCTGGA CGATGTGTAC
    GCCGAGTCGG TGGAGGCCGT GCAGGACCTC ATCAAGCGCT TTGGCCAACA GCAGCAGACG
    ACCCTGCAAG TGACGGTCAA CGTGATCAAG GAAGGGGAGG ACCTCATACA GCAGCTGAGG
    GACTCAGCCA TCTCCAGCAA CAAGACCCCC CACAACAGCT CCATCAGTCA CATCGAGACG
    GTGCTTCAGC AGCTGGACGA GGCCCAGGCC CAGATGGAGG AGCTCTTCCA GGAGCGCAAA
    ATCAAGCTGG AGCTCTTCCT GCAGCTGCGC ATATTCGAGA GGGATGCCAT CGATATCATC
    TCAGACCTTG AGTCTTGGAA CGATGAGCTT TCTCAGCAAA TGAACGACTT TGACACAGAA
    GATCTCACAA TAGCAGAACA GCGCCTCCAG CACCATGCAG ACAAAGCTTT AACCATGAAC
    AACTTGACCT TTGATGTCAT CCACCAAGGG CAGGACCTCT TGCAGTATGT CAATGAAGTG
    CAGGCCTCTG GTGTGGAGCT GCTTTGCGAT AGAGATGTCG ACATGGCCAC TCGGGTCCAG
    GACCTCCTGG AGTTCCTTCA TGAAAAGCAG CAGGAACTGG ATTTAGCAGC AGAACAGCAT
    CGGAAGCACT TGGAGCAGTG TGTGCAGCTT CGCCATCTGC AGGCTGAAGT GAAGCAGGTG
    CTGGGCTGGA TCCGCAATGG AGAGTCAATG CTGAATGCTG GACTCATCAC GGCCAGCTCA
    TTACAGGAGG CGGAGCAGCT GCAGAGGGAG CACGAGCAGT TCCAGCACGC CATCGAGAAA
    ACACATCAAA GCGCCTTGCA GGTGCAGCAG AAGGCAGAAG CCATGCTGCA GGCCAATCAC
    TATGACATGG ACATGATCAG GGACTGCGCC GAGAAAGTGG CCTCTCACTG GCAGCAGCTC
    ATGCTCAAGA TGGAAGACCG CCTCAAGCTT GTGAATGCAT CTGTGGCCTT CTACAAAACC
    TCAGAGCAGG TCTGCAGTGT CCTCGAGAGC CTGGAGCAGG AGTACAAGAG AGAAGAGGAC
    TGGTGTGGAG GAGCCGACAA ACTGGGGCCC AACTCAGAGA CGGACCATGT CACCCCAATG
    ATCAGCAAGC ATCTGGAGCA GAAAGAAGCA TTCCTGAAGG CTTGCACGCT TGCTAGAAGG
    AATGCAGATG TCTTCCTGAA ATACCTGCAC AGGAATAGTG TGAACATGCC GGGAATGGTG
    ACACATATCA AAGCCCCTGA ACAACAAGTG AAAAATATCT TGAATGAACT TTTCCAACGG
    GAGAACAGGG TACTGCATTA TTGGACCATG AGAAAGAGAC GACTAGACCA GTGCCAGCAG
    TACGTGGTCT TTGAACGAAG TGCCAAGCAG GCTTTGGAAT GGATCCATGA CAATGGCGAG
    TTCTACCTCT CCACACACAC GTCCACGGGC TCCAGTATAC AGCACACCCA GGAGCTACTG
    AAAGAGCACG AGGAGTTTCA GATCACGGCA AAGCAAACCA AAGAGAGAGT GAAGCTATTG
    ATACAGCTGG CTGATGGCTT TTGTGAAAAA GGGCATGCCC ATGCAGCAGA GATAAAAAAA
    TGTGTCACTG CAGTGGATAA GAGGTACAGA GATTTCTCTC TGCGGATGGA GAAGTACAGG
    ACCTCTTTGG AGAAAGCCCT GGGGATTTCT TCAGATTCCA ACAAATCGAG CAAAAGTCTT
    CAGCTAGACA TAATCCCTGC CAGTGTCCCT GGGTCAGAGG TGAAGCTTCG TGATGCTGCT
    CATGAACTTA ATGAAGAGAA ACGGAAGTCG GCCCGCAGAA AAGAGTTCAT AATGGCTGAG
    CTGATTCAGA CTGAAAAGGC TTACGTGAGA GACCTCCGGG AGTGTATGGA TACATACCTG
    TGGGAAATGA CCAGCGGAGT GGAGGAGATC CCGGCCGGCA TCGTAAACAG AGAGCTTGTT
    ATCTTCGGGA ACATGCAGGA AATCTATGAG TTTCATAATA ACATATTCCT AAAAGAGCTG
    GAAAAATATG AGCAGTTGCC AGAAGATGTT GGACATTGCT TTGTTACTTG GGCAGACAAG
    TTTCAGATGT ATGTCACATA TTGCAAAAAT AAGCCTGATT CTACTCAGCT GATATTAGAG
    CATGCAGGAT CCTACTTTGA TGAAATACAA CAACGGCATG GATTAGCCAA TTCCATCTCT
    TCCTACCTTA TTAAGCCAGT TCAGCGAATA ACGAAGTATC AACTTCTTTT AAAAGAACTA
    CTTACTTGCT GTGAGGAAGG AAAGGGAGAG ATAAAAGATG GCCTGGAGGT GATGCTCAGT
    GTGCCAAAAC GAGCCAATGA TGCCATGCAC CTCAGCATGC TGGAAGGGTT TGATGAAAAT
    ATTGAGTCTC AGGGAGAACT CATTCTACAA GAATCCTTCC AAGTGTGGGA CCCAAAAACC
    TTAATTCGAA AGGGTCGAGA ACGGCATCTC TTCCTTTTTG AAATGTCCTT AGTATTTAGT
    AAAGAAGTGA AAGATTCCAG TGGCAGAAGC AAGTACCTTT ATAAAAGCAA ATTGTTTACC
    TCAGAGTTGG GTGTCACAGA ACACGTTGAA GGAGATCCTT GCAAATTTGC ACTGTGGGTG
    GGGAGGACAC CAACCTCAGA TAATAAAATC GTCCTTAAGG CTTCCAGTAT AGAAAACAAG
    CAGGACTGGA TAAAGCACAT CCGCGAGGTC ATACAGGAAA GGACCATTCA CCTGAAAGGA
    GCCCTGAAGG AGCCCATTCA CATTCCCAAA ACGGCGCCGG CCACCAGACA GAAGGGCAGG
    AGGGATGGAG AGGATCTGGA TAGCCAGGGC GATGGGAGCA GCCAGCCTGA CACAATCTCT
    ATCGCCTCGA GGACGTCTCA GAACACACTG GATAGTGATA AGCTCTCTGG CGGCTGCGAG
    CTGACAGTGG TGATCCACGA CTTTACCGCC TGCAACAGCA CAGAGCTGAC CATCCGCCGT
    GGCCAGACCG TGGAGGTTTT GGAGCGGCCC CACGACAAGC CTGACTGGTG TCTGGTGCGG
    ACCACCGACC GGTCCCCTGC TGCAGAAGGC CTGGTTCCCT GTGGCTCTCT CTGTATCGCC
    CACTCGAGAA GTAGCATGGA AATGGAGGGT ATCTTCAACC ACAAAGGTAA CTGTGACAAG
    ACTACTTTTC ACGTGTGTCT ACATGGGTCA GAAATGGGCT CCTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022270065.1
    CDS21..5108
    Translation

    Target ORF information:

    RefSeq Version XM_022414357.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022414357.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAAAGCTA TGGATGTTTT GCCAATTTTA AAGGAAAAAG TTGCATACCT TTCAGGTGGC 
    AGAGATAAAC GTGGAGGTCC CATTTTAACA TTTCCTGCTC GCAGCAATCA TGATAGAATA
    CGACAGGAGG ACCTCAGGAG ACTCATTTCC TATCTAGCCT GTATCCCTAG TGAAGAGGTC
    TGCAAGCGAG GATTCACGGT GATCGTGGAC ATGCGTGGGT CCAAGTGGGA CTCCATTAAG
    CCTCTGCTGA AGATATTGCA AGAGTCCTTT CCCTGCTGCA TTCATGTTGC ACTGATAATC
    AAGCCAGACA ACTTTTGGCA GAAACAGAGG ACTAATTTTG GCAGTTCTAA GTTTGAATTT
    GAGACAAATA TGGTCTCTTT AGAAGGCCTT ACCAAAGTAG TTGATCCTTC TCAGCTAACT
    CCCGAGTTTG ATGGCTGCTT GGAATACAAC CATGAAGAAT GGATTGAAAT CAGAGTTGCT
    TTTGAAGACT ACATTAGCAA TGCAACCCAC ATGCTCTCTC GGCTGGAGGA ACTTCAGGAC
    ATCCTAGCCA AGAAAGAATT GCCTCAGGAT TTAGAGGGGG CTCGGAATAT GATTGAGGAA
    CATTCCCAGC TAAAGAAGAA GGTGATTAAG GCCCCTATAG AAGACTTAGA TTTAGAGGGA
    CAGAAGCTGC TCCAGAGGAT TCAGAGCAGT GACAGCTTTC CCAAAAAGAA CTCTGGGTCA
    GGCAATGCGG ACCTGCAGAG CCTCCTACCC AAGGTGTCCG CCATGTTGGA CAGGCTGCAC
    TCAACACGGC AGCATCTGCA TCAGATGTGG CATGTACGAA AACTGAAGCT GGACCAGTGC
    TTTCAGCTAA GGCTGTTTGA ACAGGACGCT GAAAAGATGT TTGACTGGAT CACACATAAC
    AAGGGCCTGT TTCTGAACAG CTACACTGAA ATTGGGACCA GCCATCCTCA TGCAATGGAA
    CTCCAGACAC AGCACAATCA CTTTGCCATG AACTGTATGA ACGTGTATGT TAATATAAAT
    CGCATAATGT CAGTGGCAAA TCGCTTGGTA GAGTCTGGCC ACTACGCCTC TCAGCAGATT
    AAGCAGATTG CGAATCAGCT GGAGCAGGAG TGGAAGGCAT TTGCGGCAGC CCTGGATGAG
    CGGAGTACCT TGCTGGACAT GTCCTCCATT TTCCACCAGA AGGCTGAAAA GTATATGAGC
    AACGTGGATT CGTGGTGTAA AGCCTGTGGT GAGGTAGACC TTCCCTCGGA GCTGCAGGAT
    CTGGAGGATG CCATTCACCA CCACCAGGGA ATATATGAGC ACATCACCCT TGCTTATTCC
    GAGGTGAGCC AGGATGGGAA GTCACTCCTC GACAAGCTCC AGCGGCCTTT GACTCCTGGC
    AGCTCCGACT CCCTCACGGC CTCTGCCAAC TACTCCAAGG CTGTGCACCA TGTCCTTGAT
    GTCATCCATG AGGTGCTGCA CCACCAGCGA CAGCTTGAGA ACATCTGGCA GCACCGCAAG
    GTCCGGCTCC ACCAGCGGCT GCAGCTGTGT GTGTTCCAGC AAGACGTTCA GCAGGTGCTG
    GACTGGATTG AGAACCATGG AGAAGCATTT CTGAGCAAAC ATACAGGTGT GGGGAAATCT
    CTTCACCGGG CCAGAGCTTT GCAGAAACGT CATGAAGATT TTGAAGAAGT AGCACAGAAC
    ACATATACCA ACGCAGATAA ATTACTAGAA GCAGCAGAAC AGCTGGCTCA GACCGGGGAA
    TGTGACCCAG AAGAGATTTA TCAGGCTGCG CATCAGCTTG AAGACCGGAT ACAAGATTTT
    GTTCGGCGCG TAGAGCAGCG CAAGATCCTG CTGGACATGT CAGTGTCGTT TCATAGCCAC
    GTCAAGGAGC TGTGGACATG GCTGGAGGAG CTGCAGAAGG AGCTGCTGGA CGATGTGTAC
    GCCGAGTCGG TGGAGGCCGT GCAGGACCTC ATCAAGCGCT TTGGCCAACA GCAGCAGACG
    ACCCTGCAAG TGACGGTCAA CGTGATCAAG GAAGGGGAGG ACCTCATACA GCAGCTGAGG
    GACTCAGCCA TCTCCAGCAA CAAGACCCCC CACAACAGCT CCATCAGTCA CATCGAGACG
    GTGCTTCAGC AGCTGGACGA GGCCCAGGCC CAGATGGAGG AGCTCTTCCA GGAGCGCAAA
    ATCAAGCTGG AGCTCTTCCT GCAGCTGCGC ATATTCGAGA GGGATGCCAT CGATATCATC
    TCAGACCTTG AGTCTTGGAA CGATGAGCTT TCTCAGCAAA TGAACGACTT TGACACAGAA
    GATCTCACAA TAGCAGAACA GCGCCTCCAG CACCATGCAG ACAAAGCTTT AACCATGAAC
    AACTTGACCT TTGATGTCAT CCACCAAGGG CAGGACCTCT TGCAGTATGT CAATGAAGTG
    CAGGCCTCTG GTGTGGAGCT GCTTTGCGAT AGAGATGTCG ACATGGCCAC TCGGGTCCAG
    GACCTCCTGG AGTTCCTTCA TGAAAAGCAG CAGGAACTGG ATTTAGCAGC AGAACAGCAT
    CGGAAGCACT TGGAGCAGTG TGTGCAGCTT CGCCATCTGC AGGCTGAAGT GAAGCAGGTG
    CTGGGCTGGA TCCGCAATGG AGAGTCAATG CTGAATGCTG GACTCATCAC GGCCAGCTCA
    TTACAGGAGG CGGAGCAGCT GCAGAGGGAG CACGAGCAGT TCCAGCACGC CATCGAGAAA
    ACACATCAAA GCGCCTTGCA GGTGCAGCAG AAGGCAGAAG CCATGCTGCA GGCCAATCAC
    TATGACATGG ACATGATCAG GGACTGCGCC GAGAAAGTGG CCTCTCACTG GCAGCAGCTC
    ATGCTCAAGA TGGAAGACCG CCTCAAGCTT GTGAATGCAT CTGTGGCCTT CTACAAAACC
    TCAGAGCAGG TCTGCAGTGT CCTCGAGAGC CTGGAGCAGG AGTACAAGAG AGAAGAGGAC
    TGGTGTGGAG GAGCCGACAA ACTGGGGCCC AACTCAGAGA CGGACCATGT CACCCCAATG
    ATCAGCAAGC ATCTGGAGCA GAAAGAAGCA TTCCTGAAGG CTTGCACGCT TGCTAGAAGG
    AATGCAGATG TCTTCCTGAA ATACCTGCAC AGGAATAGTG TGAACATGCC GGGAATGGTG
    ACACATATCA AAGCCCCTGA ACAACAAGTG AAAAATATCT TGAATGAACT TTTCCAACGG
    GAGAACAGGG TACTGCATTA TTGGACCATG AGAAAGAGAC GACTAGACCA GTGCCAGCAG
    TACGTGGTCT TTGAACGAAG TGCCAAGCAG GCTTTGGAAT GGATCCATGA CAATGGCGAG
    TTCTACCTCT CCACACACAC GTCCACGGGC TCCAGTATAC AGCACACCCA GGAGCTACTG
    AAAGAGCACG AGGAGTTTCA GATCACGGCA AAGCAAACCA AAGAGAGAGT GAAGCTATTG
    ATACAGCTGG CTGATGGCTT TTGTGAAAAA GGGCATGCCC ATGCAGCAGA GATAAAAAAA
    TGTGTCACTG CAGTGGATAA GAGGTACAGA GATTTCTCTC TGCGGATGGA GAAGTACAGG
    ACCTCTTTGG AGAAAGCCCT GGGGATTTCT TCAGATTCCA ACAAATCGAG CAAAAGTCTT
    CAGCTAGACA TAATCCCTGC CAGTGTCCCT GGGTCAGAGG TGAAGCTTCG TGATGCTGCT
    CATGAACTTA ATGAAGAGAA ACGGAAGTCG GCCCGCAGAA AAGAGTTCAT AATGGCTGAG
    CTGATTCAGA CTGAAAAGGC TTACGTGAGA GACCTCCGGG AGTGTATGGA TACATACCTG
    TGGGAAATGA CCAGCGGAGT GGAGGAGATC CCGGCCGGCA TCGTAAACAG AGAGCTTGTT
    ATCTTCGGGA ACATGCAGGA AATCTATGAG TTTCATAATA ACATATTCCT AAAAGAGCTG
    GAAAAATATG AGCAGTTGCC AGAAGATGTT GGACATTGCT TTGTTACTTG GGCAGACAAG
    TTTCAGATGT ATGTCACATA TTGCAAAAAT AAGCCTGATT CTACTCAGCT GATATTAGAG
    CATGCAGGAT CCTACTTTGA TGAAATACAA CAACGGCATG GATTAGCCAA TTCCATCTCT
    TCCTACCTTA TTAAGCCAGT TCAGCGAATA ACGAAGTATC AACTTCTTTT AAAAGAACTA
    CTTACTTGCT GTGAGGAAGG AAAGGGAGAG ATAAAAGATG GCCTGGAGGT GATGCTCAGT
    GTGCCAAAAC GAGCCAATGA TGCCATGCAC CTCAGCATGC TGGAAGGGTT TGATGAAAAT
    ATTGAGTCTC AGGGAGAACT CATTCTACAA GAATCCTTCC AAGTGTGGGA CCCAAAAACC
    TTAATTCGAA AGGGTCGAGA ACGGCATCTC TTCCTTTTTG AAATGTCCTT AGTATTTAGT
    AAAGAAGTGA AAGATTCCAG TGGCAGAAGC AAGTACCTTT ATAAAAGCAA ATTGTTTACC
    TCAGAGTTGG GTGTCACAGA ACACGTTGAA GGAGATCCTT GCAAATTTGC ACTGTGGGTG
    GGGAGGACAC CAACCTCAGA TAATAAAATC GTCCTTAAGG CTTCCAGTAT AGAAAACAAG
    CAGGACTGGA TAAAGCACAT CCGCGAGGTC ATACAGGAAA GGACCATTCA CCTGAAAGGA
    GCCCTGAAGG AGCCCATTCA CATTCCCAAA ACGGCGCCGG CCACCAGACA GAAGGGCAGG
    AGGGATGGAG AGGATCTGGA TAGCCAGGGC GATGGGAGCA GCCAGCCTGA CACAATCTCT
    ATCGCCTCGA GGACGTCTCA GAACACACTG GATAGTGATA AGCTCTCTGG CGGCTGCGAG
    CTGACAGTGG TGATCCACGA CTTTACCGCC TGCAACAGCA CAGAGCTGAC CATCCGCCGT
    GGCCAGACCG TGGAGGTTTT GGAGCGGCCC CACGACAAGC CTGACTGGTG TCTGGTGCGG
    ACCACCGACC GGTCCCCTGC TGCAGAAGGC CTGGTTCCCT GTGGCTCTCT CTGTATCGCC
    CACTCGAGAA GTAGCATGGA AATGGAGGGT ATCTTCAACC ACAAAGGTAA CTGTGACAAG
    ACTACTTTTC ACGTGTGTCT ACATGGGTCA GAAATGGGCT CCTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe284507
    Clone ID Related Accession (Same CDS sequence) XM_022414358.1
    Accession Version XM_022414358.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5067bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product triple functional domain protein isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAAAGCTA TGGATGTTTT GCCAATTTTA AAGGAAAAAG TTGCATACCT TTCAGGTGGC 
    AGAGATAAAC GTGGAGGTCC CATTTTAACA TTTCCTGCTC GCAGCAATCA TGATAGAATA
    CGACAGGAGG ACCTCAGGAG ACTCATTTCC TATCTAGCCT GTATCCCTAG TGAAGAGGTC
    TGCAAGCGAG GATTCACGGT GATCGTGGAC ATGCGTGGGT CCAAGTGGGA CTCCATTAAG
    CCTCTGCTGA AGATATTGCA AGAGTCCTTT CCCTGCTGCA TTCATGTTGC ACTGATAATC
    AAGCCAGACA ACTTTTGGCA GAAACAGAGG ACTAATTTTG GCAGTTCTAA GTTTGAATTT
    GAGACAAATA TGGTCTCTTT AGAAGGCCTT ACCAAAGTAG TTGATCCTTC TCAGCTAACT
    CCCGAGTTTG ATGGCTGCTT GGAATACAAC CATGAAGAAT GGATTGAAAT CAGAGTTGCT
    TTTGAAGACT ACATTAGCAA TGCAACCCAC ATGCTCTCTC GGCTGGAGGA ACTTCAGGAC
    ATCCTAGCCA AGAAAGAATT GCCTCAGGAT TTAGAGGGGG CTCGGAATAT GATTGAGGAA
    CATTCCCAGC TAAAGAAGAA GGTGATTAAG GCCCCTATAG AAGACTTAGA TTTAGAGGGA
    CAGAAGCTGC TCCAGAGGAT TCAGAGCAGT GACAGCTTTC CCAAAAAGAA CTCTGGGTCA
    GGCAATGCGG ACCTGCAGAG CCTCCTACCC AAGGTGTCCG CCATGTTGGA CAGGCTGCAC
    TCAACACGGC AGCATCTGCA TCAGATGTGG CATGTACGAA AACTGAAGCT GGACCAGTGC
    TTTCAGCTAA GGCTGTTTGA ACAGGACGCT GAAAAGATGT TTGACTGGAT CACACATAAC
    AAGGGCCTGT TTCTGAACAG CTACACTGAA ATTGGGACCA GCCATCCTCA TGCAATGGAA
    CTCCAGACAC AGCACAATCA CTTTGCCATG AACTGTATGA ACGTGTATGT TAATATAAAT
    CGCATAATGT CAGTGGCAAA TCGCTTGGTA GAGTCTGGCC ACTACGCCTC TCAGCAGATT
    AAGCAGATTG CGAATCAGCT GGAGCAGGAG TGGAAGGCAT TTGCGGCAGC CCTGGATGAG
    CGGAGTACCT TGCTGGACAT GTCCTCCATT TTCCACCAGA AGGCTGAAAA GTATATGAGC
    AACGTGGATT CGTGGTGTAA AGCCTGTGGT GAGGTAGACC TTCCCTCGGA GCTGCAGGAT
    CTGGAGGATG CCATTCACCA CCACCAGGGA ATATATGAGC ACATCACCCT TGCTTATTCC
    GAGGTGAGCC AGGATGGGAA GTCACTCCTC GACAAGCTCC AGCGGCCTTT GACTCCTGGC
    AGCTCCGACT CCCTCACGGC CTCTGCCAAC TACTCCAAGG CTGTGCACCA TGTCCTTGAT
    GTCATCCATG AGGTGCTGCA CCACCAGCGA CAGCTTGAGA ACATCTGGCA GCACCGCAAG
    GTCCGGCTCC ACCAGCGGCT GCAGCTGTGT GTGTTCCAGC AAGACGTTCA GCAGGTGCTG
    GACTGGATTG AGAACCATGG AGAAGCATTT CTGAGCAAAC ATACAGGTGT GGGGAAATCT
    CTTCACCGGG CCAGAGCTTT GCAGAAACGT CATGAAGATT TTGAAGAAGT AGCACAGAAC
    ACATATACCA ACGCAGATAA ATTACTAGAA GCAGCAGAAC AGCTGGCTCA GACCGGGGAA
    TGTGACCCAG AAGAGATTTA TCAGGCTGCG CATCAGCTTG AAGACCGGAT ACAAGATTTT
    GTTCGGCGCG TAGAGCAGCG CAAGATCCTG CTGGACATGT CAGTGTCGTT TCATAGCCAC
    GTCAAGGAGC TGTGGACATG GCTGGAGGAG CTGCAGAAGG AGCTGCTGGA CGATGTGTAC
    GCCGAGTCGG TGGAGGCCGT GCAGGACCTC ATCAAGCGCT TTGGCCAACA GCAGCAGACG
    ACCCTGCAAG TGACGGTCAA CGTGATCAAG GAAGGGGAGG ACCTCATACA GCAGCTGAGG
    GACTCAGCCA TCTCCAGCAA CAAGACCCCC CACAACAGCT CCATCAGTCA CATCGAGACG
    GTGCTTCAGC AGCTGGACGA GGCCCAGGCC CAGATGGAGG AGCTCTTCCA GGAGCGCAAA
    ATCAAGCTGG AGCTCTTCCT GCAGCTGCGC ATATTCGAGA GGGATGCCAT CGATATCATC
    TCAGACCTTG AGTCTTGGAA CGATGAGCTT TCTCAGCAAA TGAACGACTT TGACACAGAA
    GATCTCACAA TAGCAGAACA GCGCCTCCAG CACCATGCAG ACAAAGCTTT AACCATGAAC
    AACTTGACCT TTGATGTCAT CCACCAAGGG CAGGACCTCT TGCAGTATGT CAATGAAGTG
    CAGGCCTCTG GTGTGGAGCT GCTTTGCGAT AGAGATGTCG ACATGGCCAC TCGGGTCCAG
    GACCTCCTGG AGTTCCTTCA TGAAAAGCAG CAGGAACTGG ATTTAGCAGC AGAACAGCAT
    CGGAAGCACT TGGAGCAGTG TGTGCAGCTT CGCCATCTGC AGGCTGAAGT GAAGCAGGTG
    CTGGGCTGGA TCCGCAATGG AGAGTCAATG CTGAATGCTG GACTCATCAC GGCCAGCTCA
    TTACAGGAGG CGGAGCAGCT GCAGAGGGAG CACGAGCAGT TCCAGCACGC CATCGAGAAA
    ACACATCAAA GCGCCTTGCA GGTGCAGCAG AAGGCAGAAG CCATGCTGCA GGCCAATCAC
    TATGACATGG ACATGATCAG GGACTGCGCC GAGAAAGTGG CCTCTCACTG GCAGCAGCTC
    ATGCTCAAGA TGGAAGACCG CCTCAAGCTT GTGAATGCAT CTGTGGCCTT CTACAAAACC
    TCAGAGCAGG TCTGCAGTGT CCTCGAGAGC CTGGAGCAGG AGTACAAGAG AGAAGAGGAC
    TGGTGTGGAG GAGCCGACAA ACTGGGGCCC AACTCAGAGA CGGACCATGT CACCCCAATG
    ATCAGCAAGC ATCTGGAGCA GAAAGAAGCA TTCCTGAAGG CTTGCACGCT TGCTAGAAGG
    AATGCAGATG TCTTCCTGAA ATACCTGCAC AGGAATAGTG TGAACATGCC GGGAATGGTG
    ACACATATCA AAGCCCCTGA ACAACAAGTG AAAAATATCT TGAATGAACT TTTCCAACGG
    GAGAACAGGG TACTGCATTA TTGGACCATG AGAAAGAGAC GACTAGACCA GTGCCAGCAG
    TACGTGGTCT TTGAACGAAG TGCCAAGCAG GCTTTGGAAT GGATCCATGA CAATGGCGAG
    TTCTACCTCT CCACACACAC GTCCACGGGC TCCAGTATAC AGCACACCCA GGAGCTACTG
    AAAGAGCACG AGGAGTTTCA GATCACGGCA AAGCAAACCA AAGAGAGAGT GAAGCTATTG
    ATACAGCTGG CTGATGGCTT TTGTGAAAAA GGGCATGCCC ATGCAGCAGA GATAAAAAAA
    TGTGTCACTG CAGTGGATAA GAGGTACAGA GATTTCTCTC TGCGGATGGA GAAGTACAGG
    ACCTCTTTGG AGAAAGCCCT GGGGATTTCT TCAGATTCCA ACAAATCGAG CAAAAGTCTT
    CAGCTAGACA TAATCCCTGC CAGTGTCCCT GGGTCAGAGG TGAAGCTTCG TGATGCTGCT
    CATGAACTTA ATGAAGAGAA ACGGAAGTCG GCCCGCAGAA AAGAGTTCAT AATGGCTGAG
    CTGATTCAGA CTGAAAAGGC TTACGTGAGA GACCTCCGGG AGTGTATGGA TACATACCTG
    TGGGAAATGA CCAGCGGAGT GGAGGAGATC CCGGCCGGCA TCGTAAACAG AGAGCTTGTT
    ATCTTCGGGA ACATGCAGGA AATCTATGAG TTTCATAATA ACATATTCCT AAAAGAGCTG
    GAAAAATATG AGCAGTTGCC AGAAGATGTT GGACATTGCT TTGTTACTTG GGCAGACAAG
    TTTCAGATGT ATGTCACATA TTGCAAAAAT AAGCCTGATT CTACTCAGCT GATATTAGAG
    CATGCAGGAT CCTACTTTGA TGAAATACAA CAACGGCATG GATTAGCCAA TTCCATCTCT
    TCCTACCTTA TTAAGCCAGT TCAGCGAATA ACGAAGTATC AACTTCTTTT AAAAGAACTA
    CTTACTTGCT GTGAGGAAGG AAAGGGAGAG ATAAAAGATG GCCTGGAGGT GATGCTCAGT
    GTGCCAAAAC GAGCCAATGA TGCCATGCAC CTCAGCATGC TGGAAGGGTT TGATGAAAAT
    ATTGAGTCTC AGGGAGAACT CATTCTACAA GAATCCTTCC AAGTGTGGGA CCCAAAAACC
    TTAATTCGAA AGGGTCGAGA ACGGCATCTC TTCCTTTTTG AAATGTCCTT AGTATTTAGT
    AAAGAAGTGA AAGATTCCAG TGGCAGAAGC AAGTACCTTT ATAAAAGCAA ATTGTTTACC
    TCAGAGTTGG GTGTCACAGA ACACGTTGAA GGAGATCCTT GCAAATTTGC ACTGTGGGTG
    GGGAGGACAC CAACCTCAGA TAATAAAATC GTCCTTAAGG CTTCCAGTAT AGAAAACAAG
    CAGGACTGGA TAAAGCACAT CCGCGAGGTC ATACAGGAAA GGACCATTCA CCTGAAAGGA
    GCCCTGAAGG AGCCCATTCA CATTCCCAAA ACGGCGCCGG CCACCAGACA GAAGGGCAGG
    AGGGATGGAG AGGATCTGGA TAGCCAGGGC GATGGGAGCA GCCAGCCTGA CACAATCTCT
    ATCGCCTCGA GGACGTCTCA GAACACACTG GATAGTGATA AGCTCTCTGG CGGCTGCGAG
    CTGACAGTGG TGATCCACGA CTTTACCGCC TGCAACAGCA CAGAGCTGAC CATCCGCCGT
    GGCCAGACCG TGGAGGTTTT GGAGCGGCCC CACGACAAGC CTGACTGGTG TCTGGTGCGG
    ACCACCGACC GGTCCCCTGC TGCAGAAGGC CTGGTTCCCT GTGGCTCTCT CTGTATCGCC
    CACTCGAGAA GTAGCATGGA AATGGAGGGT ATCTTCAACC ACAAAGGACA CATGGAATCA
    ATTCCAGTGC TGTACATATT CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022270066.1
    CDS21..5087
    Translation

    Target ORF information:

    RefSeq Version XM_022414358.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022414358.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAAAGCTA TGGATGTTTT GCCAATTTTA AAGGAAAAAG TTGCATACCT TTCAGGTGGC 
    AGAGATAAAC GTGGAGGTCC CATTTTAACA TTTCCTGCTC GCAGCAATCA TGATAGAATA
    CGACAGGAGG ACCTCAGGAG ACTCATTTCC TATCTAGCCT GTATCCCTAG TGAAGAGGTC
    TGCAAGCGAG GATTCACGGT GATCGTGGAC ATGCGTGGGT CCAAGTGGGA CTCCATTAAG
    CCTCTGCTGA AGATATTGCA AGAGTCCTTT CCCTGCTGCA TTCATGTTGC ACTGATAATC
    AAGCCAGACA ACTTTTGGCA GAAACAGAGG ACTAATTTTG GCAGTTCTAA GTTTGAATTT
    GAGACAAATA TGGTCTCTTT AGAAGGCCTT ACCAAAGTAG TTGATCCTTC TCAGCTAACT
    CCCGAGTTTG ATGGCTGCTT GGAATACAAC CATGAAGAAT GGATTGAAAT CAGAGTTGCT
    TTTGAAGACT ACATTAGCAA TGCAACCCAC ATGCTCTCTC GGCTGGAGGA ACTTCAGGAC
    ATCCTAGCCA AGAAAGAATT GCCTCAGGAT TTAGAGGGGG CTCGGAATAT GATTGAGGAA
    CATTCCCAGC TAAAGAAGAA GGTGATTAAG GCCCCTATAG AAGACTTAGA TTTAGAGGGA
    CAGAAGCTGC TCCAGAGGAT TCAGAGCAGT GACAGCTTTC CCAAAAAGAA CTCTGGGTCA
    GGCAATGCGG ACCTGCAGAG CCTCCTACCC AAGGTGTCCG CCATGTTGGA CAGGCTGCAC
    TCAACACGGC AGCATCTGCA TCAGATGTGG CATGTACGAA AACTGAAGCT GGACCAGTGC
    TTTCAGCTAA GGCTGTTTGA ACAGGACGCT GAAAAGATGT TTGACTGGAT CACACATAAC
    AAGGGCCTGT TTCTGAACAG CTACACTGAA ATTGGGACCA GCCATCCTCA TGCAATGGAA
    CTCCAGACAC AGCACAATCA CTTTGCCATG AACTGTATGA ACGTGTATGT TAATATAAAT
    CGCATAATGT CAGTGGCAAA TCGCTTGGTA GAGTCTGGCC ACTACGCCTC TCAGCAGATT
    AAGCAGATTG CGAATCAGCT GGAGCAGGAG TGGAAGGCAT TTGCGGCAGC CCTGGATGAG
    CGGAGTACCT TGCTGGACAT GTCCTCCATT TTCCACCAGA AGGCTGAAAA GTATATGAGC
    AACGTGGATT CGTGGTGTAA AGCCTGTGGT GAGGTAGACC TTCCCTCGGA GCTGCAGGAT
    CTGGAGGATG CCATTCACCA CCACCAGGGA ATATATGAGC ACATCACCCT TGCTTATTCC
    GAGGTGAGCC AGGATGGGAA GTCACTCCTC GACAAGCTCC AGCGGCCTTT GACTCCTGGC
    AGCTCCGACT CCCTCACGGC CTCTGCCAAC TACTCCAAGG CTGTGCACCA TGTCCTTGAT
    GTCATCCATG AGGTGCTGCA CCACCAGCGA CAGCTTGAGA ACATCTGGCA GCACCGCAAG
    GTCCGGCTCC ACCAGCGGCT GCAGCTGTGT GTGTTCCAGC AAGACGTTCA GCAGGTGCTG
    GACTGGATTG AGAACCATGG AGAAGCATTT CTGAGCAAAC ATACAGGTGT GGGGAAATCT
    CTTCACCGGG CCAGAGCTTT GCAGAAACGT CATGAAGATT TTGAAGAAGT AGCACAGAAC
    ACATATACCA ACGCAGATAA ATTACTAGAA GCAGCAGAAC AGCTGGCTCA GACCGGGGAA
    TGTGACCCAG AAGAGATTTA TCAGGCTGCG CATCAGCTTG AAGACCGGAT ACAAGATTTT
    GTTCGGCGCG TAGAGCAGCG CAAGATCCTG CTGGACATGT CAGTGTCGTT TCATAGCCAC
    GTCAAGGAGC TGTGGACATG GCTGGAGGAG CTGCAGAAGG AGCTGCTGGA CGATGTGTAC
    GCCGAGTCGG TGGAGGCCGT GCAGGACCTC ATCAAGCGCT TTGGCCAACA GCAGCAGACG
    ACCCTGCAAG TGACGGTCAA CGTGATCAAG GAAGGGGAGG ACCTCATACA GCAGCTGAGG
    GACTCAGCCA TCTCCAGCAA CAAGACCCCC CACAACAGCT CCATCAGTCA CATCGAGACG
    GTGCTTCAGC AGCTGGACGA GGCCCAGGCC CAGATGGAGG AGCTCTTCCA GGAGCGCAAA
    ATCAAGCTGG AGCTCTTCCT GCAGCTGCGC ATATTCGAGA GGGATGCCAT CGATATCATC
    TCAGACCTTG AGTCTTGGAA CGATGAGCTT TCTCAGCAAA TGAACGACTT TGACACAGAA
    GATCTCACAA TAGCAGAACA GCGCCTCCAG CACCATGCAG ACAAAGCTTT AACCATGAAC
    AACTTGACCT TTGATGTCAT CCACCAAGGG CAGGACCTCT TGCAGTATGT CAATGAAGTG
    CAGGCCTCTG GTGTGGAGCT GCTTTGCGAT AGAGATGTCG ACATGGCCAC TCGGGTCCAG
    GACCTCCTGG AGTTCCTTCA TGAAAAGCAG CAGGAACTGG ATTTAGCAGC AGAACAGCAT
    CGGAAGCACT TGGAGCAGTG TGTGCAGCTT CGCCATCTGC AGGCTGAAGT GAAGCAGGTG
    CTGGGCTGGA TCCGCAATGG AGAGTCAATG CTGAATGCTG GACTCATCAC GGCCAGCTCA
    TTACAGGAGG CGGAGCAGCT GCAGAGGGAG CACGAGCAGT TCCAGCACGC CATCGAGAAA
    ACACATCAAA GCGCCTTGCA GGTGCAGCAG AAGGCAGAAG CCATGCTGCA GGCCAATCAC
    TATGACATGG ACATGATCAG GGACTGCGCC GAGAAAGTGG CCTCTCACTG GCAGCAGCTC
    ATGCTCAAGA TGGAAGACCG CCTCAAGCTT GTGAATGCAT CTGTGGCCTT CTACAAAACC
    TCAGAGCAGG TCTGCAGTGT CCTCGAGAGC CTGGAGCAGG AGTACAAGAG AGAAGAGGAC
    TGGTGTGGAG GAGCCGACAA ACTGGGGCCC AACTCAGAGA CGGACCATGT CACCCCAATG
    ATCAGCAAGC ATCTGGAGCA GAAAGAAGCA TTCCTGAAGG CTTGCACGCT TGCTAGAAGG
    AATGCAGATG TCTTCCTGAA ATACCTGCAC AGGAATAGTG TGAACATGCC GGGAATGGTG
    ACACATATCA AAGCCCCTGA ACAACAAGTG AAAAATATCT TGAATGAACT TTTCCAACGG
    GAGAACAGGG TACTGCATTA TTGGACCATG AGAAAGAGAC GACTAGACCA GTGCCAGCAG
    TACGTGGTCT TTGAACGAAG TGCCAAGCAG GCTTTGGAAT GGATCCATGA CAATGGCGAG
    TTCTACCTCT CCACACACAC GTCCACGGGC TCCAGTATAC AGCACACCCA GGAGCTACTG
    AAAGAGCACG AGGAGTTTCA GATCACGGCA AAGCAAACCA AAGAGAGAGT GAAGCTATTG
    ATACAGCTGG CTGATGGCTT TTGTGAAAAA GGGCATGCCC ATGCAGCAGA GATAAAAAAA
    TGTGTCACTG CAGTGGATAA GAGGTACAGA GATTTCTCTC TGCGGATGGA GAAGTACAGG
    ACCTCTTTGG AGAAAGCCCT GGGGATTTCT TCAGATTCCA ACAAATCGAG CAAAAGTCTT
    CAGCTAGACA TAATCCCTGC CAGTGTCCCT GGGTCAGAGG TGAAGCTTCG TGATGCTGCT
    CATGAACTTA ATGAAGAGAA ACGGAAGTCG GCCCGCAGAA AAGAGTTCAT AATGGCTGAG
    CTGATTCAGA CTGAAAAGGC TTACGTGAGA GACCTCCGGG AGTGTATGGA TACATACCTG
    TGGGAAATGA CCAGCGGAGT GGAGGAGATC CCGGCCGGCA TCGTAAACAG AGAGCTTGTT
    ATCTTCGGGA ACATGCAGGA AATCTATGAG TTTCATAATA ACATATTCCT AAAAGAGCTG
    GAAAAATATG AGCAGTTGCC AGAAGATGTT GGACATTGCT TTGTTACTTG GGCAGACAAG
    TTTCAGATGT ATGTCACATA TTGCAAAAAT AAGCCTGATT CTACTCAGCT GATATTAGAG
    CATGCAGGAT CCTACTTTGA TGAAATACAA CAACGGCATG GATTAGCCAA TTCCATCTCT
    TCCTACCTTA TTAAGCCAGT TCAGCGAATA ACGAAGTATC AACTTCTTTT AAAAGAACTA
    CTTACTTGCT GTGAGGAAGG AAAGGGAGAG ATAAAAGATG GCCTGGAGGT GATGCTCAGT
    GTGCCAAAAC GAGCCAATGA TGCCATGCAC CTCAGCATGC TGGAAGGGTT TGATGAAAAT
    ATTGAGTCTC AGGGAGAACT CATTCTACAA GAATCCTTCC AAGTGTGGGA CCCAAAAACC
    TTAATTCGAA AGGGTCGAGA ACGGCATCTC TTCCTTTTTG AAATGTCCTT AGTATTTAGT
    AAAGAAGTGA AAGATTCCAG TGGCAGAAGC AAGTACCTTT ATAAAAGCAA ATTGTTTACC
    TCAGAGTTGG GTGTCACAGA ACACGTTGAA GGAGATCCTT GCAAATTTGC ACTGTGGGTG
    GGGAGGACAC CAACCTCAGA TAATAAAATC GTCCTTAAGG CTTCCAGTAT AGAAAACAAG
    CAGGACTGGA TAAAGCACAT CCGCGAGGTC ATACAGGAAA GGACCATTCA CCTGAAAGGA
    GCCCTGAAGG AGCCCATTCA CATTCCCAAA ACGGCGCCGG CCACCAGACA GAAGGGCAGG
    AGGGATGGAG AGGATCTGGA TAGCCAGGGC GATGGGAGCA GCCAGCCTGA CACAATCTCT
    ATCGCCTCGA GGACGTCTCA GAACACACTG GATAGTGATA AGCTCTCTGG CGGCTGCGAG
    CTGACAGTGG TGATCCACGA CTTTACCGCC TGCAACAGCA CAGAGCTGAC CATCCGCCGT
    GGCCAGACCG TGGAGGTTTT GGAGCGGCCC CACGACAAGC CTGACTGGTG TCTGGTGCGG
    ACCACCGACC GGTCCCCTGC TGCAGAAGGC CTGGTTCCCT GTGGCTCTCT CTGTATCGCC
    CACTCGAGAA GTAGCATGGA AATGGAGGGT ATCTTCAACC ACAAAGGACA CATGGAATCA
    ATTCCAGTGC TGTACATATT CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe284504
    Clone ID Related Accession (Same CDS sequence) XM_022414355.1
    Accession Version XM_022414355.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9162bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product triple functional domain protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGAAAGCTA TGGATGTTTT GCCAATTTTA AAGGAAAAAG TTGCATACCT TTCAGGTGGC 
    AGAGATAAAC GTGGAGGTCC CATTTTAACA TTTCCTGCTC GCAGCAATCA TGATAGAATA
    CGACAGGAGG ACCTCAGGAG ACTCATTTCC TATCTAGCCT GTATCCCTAG TGAAGAGGTC
    TGCAAGCGAG GATTCACGGT GATCGTGGAC ATGCGTGGGT CCAAGTGGGA CTCCATTAAG
    CCTCTGCTGA AGATATTGCA AGAGTCCTTT CCCTGCTGCA TTCATGTTGC ACTGATAATC
    AAGCCAGACA ACTTTTGGCA GAAACAGAGG ACTAATTTTG GCAGTTCTAA GTTTGAATTT
    GAGACAAATA TGGTCTCTTT AGAAGGCCTT ACCAAAGTAG TTGATCCTTC TCAGCTAACT
    CCCGAGTTTG ATGGCTGCTT GGAATACAAC CATGAAGAAT GGATTGAAAT CAGAGTTGCT
    TTTGAAGACT ACATTAGCAA TGCAACCCAC ATGCTCTCTC GGCTGGAGGA ACTTCAGGAC
    ATCCTAGCCA AGAAAGAATT GCCTCAGGAT TTAGAGGGGG CTCGGAATAT GATTGAGGAA
    CATTCCCAGC TAAAGAAGAA GGTGATTAAG GCCCCTATAG AAGACTTAGA TTTAGAGGGA
    CAGAAGCTGC TCCAGAGGAT TCAGAGCAGT GACAGCTTTC CCAAAAAGAA CTCTGGGTCA
    GGCAATGCGG ACCTGCAGAG CCTCCTACCC AAGGTGTCCG CCATGTTGGA CAGGCTGCAC
    TCAACACGGC AGCATCTGCA TCAGATGTGG CATGTACGAA AACTGAAGCT GGACCAGTGC
    TTTCAGCTAA GGCTGTTTGA ACAGGACGCT GAAAAGATGT TTGACTGGAT CACACATAAC
    AAGGGCCTGT TTCTGAACAG CTACACTGAA ATTGGGACCA GCCATCCTCA TGCAATGGAA
    CTCCAGACAC AGCACAATCA CTTTGCCATG AACTGTATGA ACGTGTATGT TAATATAAAT
    CGCATAATGT CAGTGGCAAA TCGCTTGGTA GAGTCTGGCC ACTACGCCTC TCAGCAGATT
    AAGCAGATTG CGAATCAGCT GGAGCAGGAG TGGAAGGCAT TTGCGGCAGC CCTGGATGAG
    CGGAGTACCT TGCTGGACAT GTCCTCCATT TTCCACCAGA AGGCTGAAAA GTATATGAGC
    AACGTGGATT CGTGGTGTAA AGCCTGTGGT GAGGTAGACC TTCCCTCGGA GCTGCAGGAT
    CTGGAGGATG CCATTCACCA CCACCAGGGA ATATATGAGC ACATCACCCT TGCTTATTCC
    GAGGTGAGCC AGGATGGGAA GTCACTCCTC GACAAGCTCC AGCGGCCTTT GACTCCTGGC
    AGCTCCGACT CCCTCACGGC CTCTGCCAAC TACTCCAAGG CTGTGCACCA TGTCCTTGAT
    GTCATCCATG AGGTGCTGCA CCACCAGCGA CAGCTTGAGA ACATCTGGCA GCACCGCAAG
    GTCCGGCTCC ACCAGCGGCT GCAGCTGTGT GTGTTCCAGC AAGACGTTCA GCAGGTGCTG
    GACTGGATTG AGAACCATGG AGAAGCATTT CTGAGCAAAC ATACAGGTGT GGGGAAATCT
    CTTCACCGGG CCAGAGCTTT GCAGAAACGT CATGAAGATT TTGAAGAAGT AGCACAGAAC
    ACATATACCA ACGCAGATAA ATTACTAGAA GCAGCAGAAC AGCTGGCTCA GACCGGGGAA
    TGTGACCCAG AAGAGATTTA TCAGGCTGCG CATCAGCTTG AAGACCGGAT ACAAGATTTT
    GTTCGGCGCG TAGAGCAGCG CAAGATCCTG CTGGACATGT CAGTGTCGTT TCATAGCCAC
    GTCAAGGAGC TGTGGACATG GCTGGAGGAG CTGCAGAAGG AGCTGCTGGA CGATGTGTAC
    GCCGAGTCGG TGGAGGCCGT GCAGGACCTC ATCAAGCGCT TTGGCCAACA GCAGCAGACG
    ACCCTGCAAG TGACGGTCAA CGTGATCAAG GAAGGGGAGG ACCTCATACA GCAGCTGAGG
    GACTCAGCCA TCTCCAGCAA CAAGACCCCC CACAACAGCT CCATCAGTCA CATCGAGACG
    GTGCTTCAGC AGCTGGACGA GGCCCAGGCC CAGATGGAGG AGCTCTTCCA GGAGCGCAAA
    ATCAAGCTGG AGCTCTTCCT GCAGCTGCGC ATATTCGAGA GGGATGCCAT CGATATCATC
    TCAGACCTTG AGTCTTGGAA CGATGAGCTT TCTCAGCAAA TGAACGACTT TGACACAGAA
    GATCTCACAA TAGCAGAACA GCGCCTCCAG CACCATGCAG ACAAAGCTTT AACCATGAAC
    AACTTGACCT TTGATGTCAT CCACCAAGGG CAGGACCTCT TGCAGTATGT CAATGAAGTG
    CAGGCCTCTG GTGTGGAGCT GCTTTGCGAT AGAGATGTCG ACATGGCCAC TCGGGTCCAG
    GACCTCCTGG AGTTCCTTCA TGAAAAGCAG CAGGAACTGG ATTTAGCAGC AGAACAGCAT
    CGGAAGCACT TGGAGCAGTG TGTGCAGCTT CGCCATCTGC AGGCTGAAGT GAAGCAGGTG
    CTGGGCTGGA TCCGCAATGG AGAGTCAATG CTGAATGCTG GACTCATCAC GGCCAGCTCA
    TTACAGGAGG CGGAGCAGCT GCAGAGGGAG CACGAGCAGT TCCAGCACGC CATCGAGAAA
    ACACATCAAA GCGCCTTGCA GGTGCAGCAG AAGGCAGAAG CCATGCTGCA GGCCAATCAC
    TATGACATGG ACATGATCAG GGACTGCGCC GAGAAAGTGG CCTCTCACTG GCAGCAGCTC
    ATGCTCAAGA TGGAAGACCG CCTCAAGCTT GTGAATGCAT CTGTGGCCTT CTACAAAACC
    TCAGAGCAGG TCTGCAGTGT CCTCGAGAGC CTGGAGCAGG AGTACAAGAG AGAAGAGGAC
    TGGTGTGGAG GAGCCGACAA ACTGGGGCCC AACTCAGAGA CGGACCATGT CACCCCAATG
    ATCAGCAAGC ATCTGGAGCA GAAAGAAGCA TTCCTGAAGG CTTGCACGCT TGCTAGAAGG
    AATGCAGATG TCTTCCTGAA ATACCTGCAC AGGAATAGTG TGAACATGCC GGGAATGGTG
    ACACATATCA AAGCCCCTGA ACAACAAGTG AAAAATATCT TGAATGAACT TTTCCAACGG
    GAGAACAGGG TACTGCATTA TTGGACCATG AGAAAGAGAC GACTAGACCA GTGCCAGCAG
    TACGTGGTCT TTGAACGAAG TGCCAAGCAG GCTTTGGAAT GGATCCATGA CAATGGCGAG
    TTCTACCTCT CCACACACAC GTCCACGGGC TCCAGTATAC AGCACACCCA GGAGCTACTG
    AAAGAGCACG AGGAGTTTCA GATCACGGCA AAGCAAACCA AAGAGAGAGT GAAGCTATTG
    ATACAGCTGG CTGATGGCTT TTGTGAAAAA GGGCATGCCC ATGCAGCAGA GATAAAAAAA
    TGTGTCACTG CAGTGGATAA GAGGTACAGA GATTTCTCTC TGCGGATGGA GAAGTACAGG
    ACCTCTTTGG AGAAAGCCCT GGGGATTTCT TCAGATTCCA ACAAATCGAG CAAAAGTCTT
    CAGCTAGACA TAATCCCTGC CAGTGTCCCT GGGTCAGAGG TGAAGCTTCG TGATGCTGCT
    CATGAACTTA ATGAAGAGAA ACGGAAGTCG GCCCGCAGAA AAGAGTTCAT AATGGCTGAG
    CTGATTCAGA CTGAAAAGGC TTACGTGAGA GACCTCCGGG AGTGTATGGA TACATACCTG
    TGGGAAATGA CCAGCGGAGT GGAGGAGATC CCGGCCGGCA TCGTAAACAG AGAGCTTGTT
    ATCTTCGGGA ACATGCAGGA AATCTATGAG TTTCATAATA ACATATTCCT AAAAGAGCTG
    GAAAAATATG AGCAGTTGCC AGAAGATGTT GGACATTGCT TTGTTACTTG GGCAGACAAG
    TTTCAGATGT ATGTCACATA TTGCAAAAAT AAGCCTGATT CTACTCAGCT GATATTAGAG
    CATGCAGGAT CCTACTTTGA TGAAATACAA CAACGGCATG GATTAGCCAA TTCCATCTCT
    TCCTACCTTA TTAAGCCAGT TCAGCGAATA ACGAAGTATC AACTTCTTTT AAAAGAACTA
    CTTACTTGCT GTGAGGAAGG AAAGGGAGAG ATAAAAGATG GCCTGGAGGT GATGCTCAGT
    GTGCCAAAAC GAGCCAATGA TGCCATGCAC CTCAGCATGC TGGAAGGGTT TGATGAAAAT
    ATTGAGTCTC AGGGAGAACT CATTCTACAA GAATCCTTCC AAGTGTGGGA CCCAAAAACC
    TTAATTCGAA AGGGTCGAGA ACGGCATCTC TTCCTTTTTG AAATGTCCTT AGTATTTAGT
    AAAGAAGTGA AAGATTCCAG TGGCAGAAGC AAGTACCTTT ATAAAAGCAA ATTGTTTACC
    TCAGAGTTGG GTGTCACAGA ACACGTTGAA GGAGATCCTT GCAAATTTGC ACTGTGGGTG
    GGGAGGACAC CAACCTCAGA TAATAAAATC GTCCTTAAGG CTTCCAGTAT AGAAAACAAG
    CAGGACTGGA TAAAGCACAT CCGCGAGGTC ATACAGGAAA GGACCATTCA CCTGAAAGGA
    GCCCTGAAGG AGCCCATTCA CATTCCCAAA ACGGCGCCGG CCACCAGACA GAAGGGCAGG
    AGGGATGGAG AGGATCTGGA TAGCCAGGGC GATGGGAGCA GCCAGCCTGA CACAATCTCT
    ATCGCCTCGA GGACGTCTCA GAACACACTG GATAGTGATA AGCTCTCTGG CGGCTGCGAG
    CTGACAGTGG TGATCCACGA CTTTACCGCC TGCAACAGCA CAGAGCTGAC CATCCGCCGT
    GGCCAGACCG TGGAGGTTTT GGAGCGGCCC CACGACAAGC CTGACTGGTG TCTGGTGCGG
    ACCACCGACC GGTCCCCTGC TGCAGAAGGC CTGGTTCCCT GTGGCTCTCT CTGTATCGCC
    CACTCGAGAA GTAGCATGGA AATGGAGGGT ATCTTCAACC ACAAAGACTC GCTGTCCGTC
    TCCAGTAACG ATGCCAGCCC GCCGGCGTCC GTGGCCTCCC TCCAGCCCCA CCTGATGGGG
    GCCCAGAGCT CGCCAGGCCC CAAGCGCCCA GGGAACACTC TGCGCAAGTG GCTGACGAGC
    CCGGTGCGGC GGCTGAGCAG CGGGAAGGCG GACGGGCACG TGAAGAAGCT GGCGCACAAG
    CACAAGAAGA GCAGGGAGGT CCGCAAGAGC ACCGACGCGG GCTCGCAGAA GGACTCGGAC
    GACAGCGCGG CCACCCCGCA GGACGAGACT GTCGAGGAGA GAGGCCGGAA TGAAGGCCTG
    AGCAGTGGCA CACTCTCGAA GTCGTCATCG TCGGGCATGC AAAGCTGTGG AGAGGAAGAA
    GGGGAGGAGG GGGCCGACGC CGTGCCTCTC CCCCCGCCCA TGGCCATCCA GCAGCACAGC
    CTCCTCCAGC CGGATTCACA GGACGACAAG GCGTCTTCTC GGCTATTAGT TCGCCCCACC
    AGCTCTGAAA CGCCAAGCGC AGCCGAGCTC GTGAGTGCAA TTGAAGAACT CGTGAAAAGC
    AAGATGGCCT TGGAGGACCG TCCCAGCTCA CTCCTTGTTG ACCAGGGAGA CAGCAGCAGC
    CCCTCCTTCA ACCCTTCAGA CAACTCCCTC CTGTCCTCCT CCTCGCCCAT TGATGAGATG
    GAAGAAAGGA AATCCAGCTC TTTAAAGAGA CGGCACTATG TTTTGCAAGA ACTAGTGGAG
    ACAGAACGTG ACTATGTGCG AGACCTCGGC TATGTAGTTG AGGGCTACAT GGCTCTCATG
    AAAGAAGATG GTGTTCCTGA TGACATGAAA GGAAAGGACA AAATTGTATT TGGCAACATC
    CATCAGATTT ATGACTGGCA CAGAGACTTT TTTTTAGGAG AGTTGGAGAA GTGCCTTGAA
    GATCCAGAAA AACTAGGATC CCTTTTTGTT AAACACGAAA GAAGGTTGCA CATGTACATA
    GTTTATTGTC AAAATAAACC AAAGTCTGAG CACATTGTCT CAGAATACAT TGATACCTTT
    TTTGAGGACT TAAAGCAGCG TCTGGGCCAC AGGTTGCAGC TCACAGATCT GTTGATCAAA
    CCAGTGCAGC GGATTATGAA GTACCAGCTG CTCCTGAAGG ACTTCCTCAA GTATTCCAAA
    AAAGCTAGCC TGGATACATC AGAATTAGAG AGAGCTGTGG AAGTCATGTG CATAGTCCCC
    AAGCGGTGCA ACGACATGAT GAACGTGGGG CGGCTGCAAG GATTTGATGG TAAAATTGTT
    GCCCAGGGCA AGCTACTCTT GCAGGACACA TTCTTGGTCA CAGACCAGGA CTCGGGACTT
    CTGCCTCGCT GTAAAGAGAG GCGAGTTTTC CTCTTTGAGC AAATCGTCAT TTTCAGTGAA
    CCACTTGATA AAAAAAAGGG CTTCTCGATG CCAGGATTCC TGTTTAAGAA CAGTATCAAG
    GTGAGTTGCC TTTGCCTGGA GGAAAATGTG GAAAATGATC CATGTAAATT TGCTTTGACG
    TCGAGGACGG GTGGTGTGGT GGAGACCTTC ATTTTGCATT CATCCAGTCC AAGTGTCCGG
    CAAACATGGA TCCATGAAAT CAACCAAATT TTAGAAAACC AGCGCAATTT TTTAAATGCC
    TTGACATCAC CAATCGAGTA TCAGAGGAAC CACAGTGGGG GCGGCGGCGG GGGCAGTGGC
    GGGGGCGGCG GCAGCGGAGG CAGCGGCGGC GGGGCTGGCA GCGGGGCCCC CGGCGGCGGA
    GGCAGCAACC CCAGCGGCGG CCCTGGCAGC TGCAGCGGCC TCCCCAGCTC GAGCAGAAGC
    CGACCCTCCC GGATCCCCCA GCCTGTCAGA CACCACTCCC CCGTGCTGGT CTCCTCTGCA
    GCCTCGGGCC AGGCAGAGGC AGACAAGATG TCAGGTACGT CCACTCCCGG CCCTGCACTG
    GCCCCTCCCG GCAGCAGCCC CGCCCCGGAG CCCGGCCCCA GCCAGCCTGG CAGGCGCGCC
    CCCGAGCCCG GAGGCCCCGA GCGAGAAGCC GAGCTCATCC CCAGGATGAA GGTGATTGAG
    AGCCCCCGGA GGTGCTCGGG GGGCGCGGCG GGCGCCGGCC GCGATGCGGA CACCAAGGAG
    GCGCGGGCCC CCCCGGAGGA CAGCCGCTCG CGGAGCAGCC CGGGCTCGCT GCCCCTGGCA
    AAGCCCAGGC CTGGGGCCAC CTCGCCTCTG AACTCTCCCG TGGCCGCCGC ATTACCTCCT
    CCAATCGGCA AGGAGCCCTG TCCCCCCAGC AGCCCCCTGC AGAAGGGGGG CTCCTTCTGG
    AGCTCCATCC CCGCCTCCCC CGCCAGCCGG CCAGGCTCCT TCACCTTCCC GGGGGACAGT
    GACTCCCTCC AGCGGCAGGC CCAGCGCCAC GCGGCCCCCA GCAAGGACAC AGACCGCATG
    AGCACCTGCT CGTCGGCCAG CGAGCAGTCG GTACAGTCCA CCCAGAGCAA CGGGAGTGAA
    AGTAGCAGCA GTAGCAACAT CTCCACCATG TTGGTGACAC ACGATTACAC GGCAGTGAAG
    GAGGATGAGA TAAACGTCTA TCAAGGAGAG GTCGTTCAAA TTCTGGCCAG CAATCAACAG
    AACATGTTTC TGGTGTTCCG AGCCGCCACT GACCAGTGCC CCGCAGCCGA GGGCTGGATT
    CCAGGCTTCG TCCTGGGGCA TACCAGTGCA GTCATCATGG AGAACCCCGA TGGGACTCTC
    AAGAAGTCAA CATCTTGGCA CACAGCACTC CGTTTAAGGA AAAAATCTGA GAAAAAAGAT
    AAAGACGGCA AGCGGGAAGG CAAGTTAGAG AATGGTTATC GGAAGTCACG GGAGGGGCTC
    AGCAGCAAGG TATCTGTGAA GCTTCTCAAT CCCAACTACA TATATGACGT TCCCCCAGAA
    TTCGTCATTC CCTTGAGTGA GGTCACGTGT GAGACAGGGG AGACCGTTGT TTTTAGATGT
    CGGGTTTGTG GCCGCCCCAA GGCCTCAATC ACCTGGAAGG GCCCTGAGCA CAACACCCTG
    AACAACGATG GTCACTACAG CATCTCCTAC AGTGACTTGG GAGAGGCCGC TTTGAAGATC
    GTCGGCGTGA CCACAGAAGA TGATGGCATC TACACATGCA TCGCTGTGAA CGACATGGGT
    TCAGCCTCGT CTTCGGCCAG TCTGAGGGTG CTGGGTCCAG GGAGTGATGG GATCATGGTG
    ACCTGGAAAG ACAACTTTGA CTCCTTCTAC AGCGAAGTGG CTGAGCTTGG CAGGGGCAGA
    TTCTCTGTCG TTAAGAAATG TGATCAGAAA GGAACCAAAC GAGCAGTGGC CACTAAATTT
    GTGAACAAGA AGTTGATGAA GCGTGACCAG GTCACCCATG AACTTGGAAT CCTGCAGAAC
    CTCCAGCACC CGCTGCTTAT TGGTCTCCTT GATACCTTCG AGACCCCCAC CAGCTACGTC
    CTGGTGTTAG AAATGGCTGA CCAGGGGCGT CTCCTGGACT GTGTGGTGCG ATGGGGAGAC
    CTCACTGAAG GGAAGATCAG GGCGTATCTG GGGGAGGTTC TGGAAGCCGT CCAATACCTT
    CACAACTGCA GGATAGCACA CCTGGACCTA AAGCCTGAAA ACATTCTGGT GGATCAGAGT
    CTGGCCAAGC CCACGATCAA ACTGGCCGAT TTTGGAGATG CTGTCCAACT CAACACGACC
    TACTACATCC ATCAGTTACT GGGGAACCCC GAGTTTGCAG CCCCCGAAAT CATCCTTGGG
    AACCCCATCT CCCTGACCTC GGATACGTGG AGTGTCGGAG TGCTCGCTTA CGTGCTTCTC
    AGCGGCGTGT CCCCCTTCCT CGACGACAGT GTGGAGGAGA CCTGCCTGAA CATTTGCCGA
    CTAGACTTTA GTTTCCCGGA GGACTGCTTT AAAGGTGTGA GCCAGAAGGC CAAGGAGTTC
    GTGTGTTTCC TTCTCCAGGA GGACCCTGCC AGGCGTCCCT CGGCTGCGCT GGCCCTGCAG
    GAGCAGTGGC TGCAGGCGGG CCACGGCGGT GGCAAAGGCG CGGGCGTCCT CGACACGTCC
    AGACTGACTT CCTTCATTGA GCGGCGCAAA CACCAGAACG ATGTTCGACC TATCCGTAGC
    GTTAAAAACT TCTTACAGAG CAGGCTTTTG CCTAGAGTTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022270063.1
    CDS21..9182
    Translation

    Target ORF information:

    RefSeq Version XM_022414355.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022414355.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGAAAGCTA TGGATGTTTT GCCAATTTTA AAGGAAAAAG TTGCATACCT TTCAGGTGGC 
    AGAGATAAAC GTGGAGGTCC CATTTTAACA TTTCCTGCTC GCAGCAATCA TGATAGAATA
    CGACAGGAGG ACCTCAGGAG ACTCATTTCC TATCTAGCCT GTATCCCTAG TGAAGAGGTC
    TGCAAGCGAG GATTCACGGT GATCGTGGAC ATGCGTGGGT CCAAGTGGGA CTCCATTAAG
    CCTCTGCTGA AGATATTGCA AGAGTCCTTT CCCTGCTGCA TTCATGTTGC ACTGATAATC
    AAGCCAGACA ACTTTTGGCA GAAACAGAGG ACTAATTTTG GCAGTTCTAA GTTTGAATTT
    GAGACAAATA TGGTCTCTTT AGAAGGCCTT ACCAAAGTAG TTGATCCTTC TCAGCTAACT
    CCCGAGTTTG ATGGCTGCTT GGAATACAAC CATGAAGAAT GGATTGAAAT CAGAGTTGCT
    TTTGAAGACT ACATTAGCAA TGCAACCCAC ATGCTCTCTC GGCTGGAGGA ACTTCAGGAC
    ATCCTAGCCA AGAAAGAATT GCCTCAGGAT TTAGAGGGGG CTCGGAATAT GATTGAGGAA
    CATTCCCAGC TAAAGAAGAA GGTGATTAAG GCCCCTATAG AAGACTTAGA TTTAGAGGGA
    CAGAAGCTGC TCCAGAGGAT TCAGAGCAGT GACAGCTTTC CCAAAAAGAA CTCTGGGTCA
    GGCAATGCGG ACCTGCAGAG CCTCCTACCC AAGGTGTCCG CCATGTTGGA CAGGCTGCAC
    TCAACACGGC AGCATCTGCA TCAGATGTGG CATGTACGAA AACTGAAGCT GGACCAGTGC
    TTTCAGCTAA GGCTGTTTGA ACAGGACGCT GAAAAGATGT TTGACTGGAT CACACATAAC
    AAGGGCCTGT TTCTGAACAG CTACACTGAA ATTGGGACCA GCCATCCTCA TGCAATGGAA
    CTCCAGACAC AGCACAATCA CTTTGCCATG AACTGTATGA ACGTGTATGT TAATATAAAT
    CGCATAATGT CAGTGGCAAA TCGCTTGGTA GAGTCTGGCC ACTACGCCTC TCAGCAGATT
    AAGCAGATTG CGAATCAGCT GGAGCAGGAG TGGAAGGCAT TTGCGGCAGC CCTGGATGAG
    CGGAGTACCT TGCTGGACAT GTCCTCCATT TTCCACCAGA AGGCTGAAAA GTATATGAGC
    AACGTGGATT CGTGGTGTAA AGCCTGTGGT GAGGTAGACC TTCCCTCGGA GCTGCAGGAT
    CTGGAGGATG CCATTCACCA CCACCAGGGA ATATATGAGC ACATCACCCT TGCTTATTCC
    GAGGTGAGCC AGGATGGGAA GTCACTCCTC GACAAGCTCC AGCGGCCTTT GACTCCTGGC
    AGCTCCGACT CCCTCACGGC CTCTGCCAAC TACTCCAAGG CTGTGCACCA TGTCCTTGAT
    GTCATCCATG AGGTGCTGCA CCACCAGCGA CAGCTTGAGA ACATCTGGCA GCACCGCAAG
    GTCCGGCTCC ACCAGCGGCT GCAGCTGTGT GTGTTCCAGC AAGACGTTCA GCAGGTGCTG
    GACTGGATTG AGAACCATGG AGAAGCATTT CTGAGCAAAC ATACAGGTGT GGGGAAATCT
    CTTCACCGGG CCAGAGCTTT GCAGAAACGT CATGAAGATT TTGAAGAAGT AGCACAGAAC
    ACATATACCA ACGCAGATAA ATTACTAGAA GCAGCAGAAC AGCTGGCTCA GACCGGGGAA
    TGTGACCCAG AAGAGATTTA TCAGGCTGCG CATCAGCTTG AAGACCGGAT ACAAGATTTT
    GTTCGGCGCG TAGAGCAGCG CAAGATCCTG CTGGACATGT CAGTGTCGTT TCATAGCCAC
    GTCAAGGAGC TGTGGACATG GCTGGAGGAG CTGCAGAAGG AGCTGCTGGA CGATGTGTAC
    GCCGAGTCGG TGGAGGCCGT GCAGGACCTC ATCAAGCGCT TTGGCCAACA GCAGCAGACG
    ACCCTGCAAG TGACGGTCAA CGTGATCAAG GAAGGGGAGG ACCTCATACA GCAGCTGAGG
    GACTCAGCCA TCTCCAGCAA CAAGACCCCC CACAACAGCT CCATCAGTCA CATCGAGACG
    GTGCTTCAGC AGCTGGACGA GGCCCAGGCC CAGATGGAGG AGCTCTTCCA GGAGCGCAAA
    ATCAAGCTGG AGCTCTTCCT GCAGCTGCGC ATATTCGAGA GGGATGCCAT CGATATCATC
    TCAGACCTTG AGTCTTGGAA CGATGAGCTT TCTCAGCAAA TGAACGACTT TGACACAGAA
    GATCTCACAA TAGCAGAACA GCGCCTCCAG CACCATGCAG ACAAAGCTTT AACCATGAAC
    AACTTGACCT TTGATGTCAT CCACCAAGGG CAGGACCTCT TGCAGTATGT CAATGAAGTG
    CAGGCCTCTG GTGTGGAGCT GCTTTGCGAT AGAGATGTCG ACATGGCCAC TCGGGTCCAG
    GACCTCCTGG AGTTCCTTCA TGAAAAGCAG CAGGAACTGG ATTTAGCAGC AGAACAGCAT
    CGGAAGCACT TGGAGCAGTG TGTGCAGCTT CGCCATCTGC AGGCTGAAGT GAAGCAGGTG
    CTGGGCTGGA TCCGCAATGG AGAGTCAATG CTGAATGCTG GACTCATCAC GGCCAGCTCA
    TTACAGGAGG CGGAGCAGCT GCAGAGGGAG CACGAGCAGT TCCAGCACGC CATCGAGAAA
    ACACATCAAA GCGCCTTGCA GGTGCAGCAG AAGGCAGAAG CCATGCTGCA GGCCAATCAC
    TATGACATGG ACATGATCAG GGACTGCGCC GAGAAAGTGG CCTCTCACTG GCAGCAGCTC
    ATGCTCAAGA TGGAAGACCG CCTCAAGCTT GTGAATGCAT CTGTGGCCTT CTACAAAACC
    TCAGAGCAGG TCTGCAGTGT CCTCGAGAGC CTGGAGCAGG AGTACAAGAG AGAAGAGGAC
    TGGTGTGGAG GAGCCGACAA ACTGGGGCCC AACTCAGAGA CGGACCATGT CACCCCAATG
    ATCAGCAAGC ATCTGGAGCA GAAAGAAGCA TTCCTGAAGG CTTGCACGCT TGCTAGAAGG
    AATGCAGATG TCTTCCTGAA ATACCTGCAC AGGAATAGTG TGAACATGCC GGGAATGGTG
    ACACATATCA AAGCCCCTGA ACAACAAGTG AAAAATATCT TGAATGAACT TTTCCAACGG
    GAGAACAGGG TACTGCATTA TTGGACCATG AGAAAGAGAC GACTAGACCA GTGCCAGCAG
    TACGTGGTCT TTGAACGAAG TGCCAAGCAG GCTTTGGAAT GGATCCATGA CAATGGCGAG
    TTCTACCTCT CCACACACAC GTCCACGGGC TCCAGTATAC AGCACACCCA GGAGCTACTG
    AAAGAGCACG AGGAGTTTCA GATCACGGCA AAGCAAACCA AAGAGAGAGT GAAGCTATTG
    ATACAGCTGG CTGATGGCTT TTGTGAAAAA GGGCATGCCC ATGCAGCAGA GATAAAAAAA
    TGTGTCACTG CAGTGGATAA GAGGTACAGA GATTTCTCTC TGCGGATGGA GAAGTACAGG
    ACCTCTTTGG AGAAAGCCCT GGGGATTTCT TCAGATTCCA ACAAATCGAG CAAAAGTCTT
    CAGCTAGACA TAATCCCTGC CAGTGTCCCT GGGTCAGAGG TGAAGCTTCG TGATGCTGCT
    CATGAACTTA ATGAAGAGAA ACGGAAGTCG GCCCGCAGAA AAGAGTTCAT AATGGCTGAG
    CTGATTCAGA CTGAAAAGGC TTACGTGAGA GACCTCCGGG AGTGTATGGA TACATACCTG
    TGGGAAATGA CCAGCGGAGT GGAGGAGATC CCGGCCGGCA TCGTAAACAG AGAGCTTGTT
    ATCTTCGGGA ACATGCAGGA AATCTATGAG TTTCATAATA ACATATTCCT AAAAGAGCTG
    GAAAAATATG AGCAGTTGCC AGAAGATGTT GGACATTGCT TTGTTACTTG GGCAGACAAG
    TTTCAGATGT ATGTCACATA TTGCAAAAAT AAGCCTGATT CTACTCAGCT GATATTAGAG
    CATGCAGGAT CCTACTTTGA TGAAATACAA CAACGGCATG GATTAGCCAA TTCCATCTCT
    TCCTACCTTA TTAAGCCAGT TCAGCGAATA ACGAAGTATC AACTTCTTTT AAAAGAACTA
    CTTACTTGCT GTGAGGAAGG AAAGGGAGAG ATAAAAGATG GCCTGGAGGT GATGCTCAGT
    GTGCCAAAAC GAGCCAATGA TGCCATGCAC CTCAGCATGC TGGAAGGGTT TGATGAAAAT
    ATTGAGTCTC AGGGAGAACT CATTCTACAA GAATCCTTCC AAGTGTGGGA CCCAAAAACC
    TTAATTCGAA AGGGTCGAGA ACGGCATCTC TTCCTTTTTG AAATGTCCTT AGTATTTAGT
    AAAGAAGTGA AAGATTCCAG TGGCAGAAGC AAGTACCTTT ATAAAAGCAA ATTGTTTACC
    TCAGAGTTGG GTGTCACAGA ACACGTTGAA GGAGATCCTT GCAAATTTGC ACTGTGGGTG
    GGGAGGACAC CAACCTCAGA TAATAAAATC GTCCTTAAGG CTTCCAGTAT AGAAAACAAG
    CAGGACTGGA TAAAGCACAT CCGCGAGGTC ATACAGGAAA GGACCATTCA CCTGAAAGGA
    GCCCTGAAGG AGCCCATTCA CATTCCCAAA ACGGCGCCGG CCACCAGACA GAAGGGCAGG
    AGGGATGGAG AGGATCTGGA TAGCCAGGGC GATGGGAGCA GCCAGCCTGA CACAATCTCT
    ATCGCCTCGA GGACGTCTCA GAACACACTG GATAGTGATA AGCTCTCTGG CGGCTGCGAG
    CTGACAGTGG TGATCCACGA CTTTACCGCC TGCAACAGCA CAGAGCTGAC CATCCGCCGT
    GGCCAGACCG TGGAGGTTTT GGAGCGGCCC CACGACAAGC CTGACTGGTG TCTGGTGCGG
    ACCACCGACC GGTCCCCTGC TGCAGAAGGC CTGGTTCCCT GTGGCTCTCT CTGTATCGCC
    CACTCGAGAA GTAGCATGGA AATGGAGGGT ATCTTCAACC ACAAAGACTC GCTGTCCGTC
    TCCAGTAACG ATGCCAGCCC GCCGGCGTCC GTGGCCTCCC TCCAGCCCCA CCTGATGGGG
    GCCCAGAGCT CGCCAGGCCC CAAGCGCCCA GGGAACACTC TGCGCAAGTG GCTGACGAGC
    CCGGTGCGGC GGCTGAGCAG CGGGAAGGCG GACGGGCACG TGAAGAAGCT GGCGCACAAG
    CACAAGAAGA GCAGGGAGGT CCGCAAGAGC ACCGACGCGG GCTCGCAGAA GGACTCGGAC
    GACAGCGCGG CCACCCCGCA GGACGAGACT GTCGAGGAGA GAGGCCGGAA TGAAGGCCTG
    AGCAGTGGCA CACTCTCGAA GTCGTCATCG TCGGGCATGC AAAGCTGTGG AGAGGAAGAA
    GGGGAGGAGG GGGCCGACGC CGTGCCTCTC CCCCCGCCCA TGGCCATCCA GCAGCACAGC
    CTCCTCCAGC CGGATTCACA GGACGACAAG GCGTCTTCTC GGCTATTAGT TCGCCCCACC
    AGCTCTGAAA CGCCAAGCGC AGCCGAGCTC GTGAGTGCAA TTGAAGAACT CGTGAAAAGC
    AAGATGGCCT TGGAGGACCG TCCCAGCTCA CTCCTTGTTG ACCAGGGAGA CAGCAGCAGC
    CCCTCCTTCA ACCCTTCAGA CAACTCCCTC CTGTCCTCCT CCTCGCCCAT TGATGAGATG
    GAAGAAAGGA AATCCAGCTC TTTAAAGAGA CGGCACTATG TTTTGCAAGA ACTAGTGGAG
    ACAGAACGTG ACTATGTGCG AGACCTCGGC TATGTAGTTG AGGGCTACAT GGCTCTCATG
    AAAGAAGATG GTGTTCCTGA TGACATGAAA GGAAAGGACA AAATTGTATT TGGCAACATC
    CATCAGATTT ATGACTGGCA CAGAGACTTT TTTTTAGGAG AGTTGGAGAA GTGCCTTGAA
    GATCCAGAAA AACTAGGATC CCTTTTTGTT AAACACGAAA GAAGGTTGCA CATGTACATA
    GTTTATTGTC AAAATAAACC AAAGTCTGAG CACATTGTCT CAGAATACAT TGATACCTTT
    TTTGAGGACT TAAAGCAGCG TCTGGGCCAC AGGTTGCAGC TCACAGATCT GTTGATCAAA
    CCAGTGCAGC GGATTATGAA GTACCAGCTG CTCCTGAAGG ACTTCCTCAA GTATTCCAAA
    AAAGCTAGCC TGGATACATC AGAATTAGAG AGAGCTGTGG AAGTCATGTG CATAGTCCCC
    AAGCGGTGCA ACGACATGAT GAACGTGGGG CGGCTGCAAG GATTTGATGG TAAAATTGTT
    GCCCAGGGCA AGCTACTCTT GCAGGACACA TTCTTGGTCA CAGACCAGGA CTCGGGACTT
    CTGCCTCGCT GTAAAGAGAG GCGAGTTTTC CTCTTTGAGC AAATCGTCAT TTTCAGTGAA
    CCACTTGATA AAAAAAAGGG CTTCTCGATG CCAGGATTCC TGTTTAAGAA CAGTATCAAG
    GTGAGTTGCC TTTGCCTGGA GGAAAATGTG GAAAATGATC CATGTAAATT TGCTTTGACG
    TCGAGGACGG GTGGTGTGGT GGAGACCTTC ATTTTGCATT CATCCAGTCC AAGTGTCCGG
    CAAACATGGA TCCATGAAAT CAACCAAATT TTAGAAAACC AGCGCAATTT TTTAAATGCC
    TTGACATCAC CAATCGAGTA TCAGAGGAAC CACAGTGGGG GCGGCGGCGG GGGCAGTGGC
    GGGGGCGGCG GCAGCGGAGG CAGCGGCGGC GGGGCTGGCA GCGGGGCCCC CGGCGGCGGA
    GGCAGCAACC CCAGCGGCGG CCCTGGCAGC TGCAGCGGCC TCCCCAGCTC GAGCAGAAGC
    CGACCCTCCC GGATCCCCCA GCCTGTCAGA CACCACTCCC CCGTGCTGGT CTCCTCTGCA
    GCCTCGGGCC AGGCAGAGGC AGACAAGATG TCAGGTACGT CCACTCCCGG CCCTGCACTG
    GCCCCTCCCG GCAGCAGCCC CGCCCCGGAG CCCGGCCCCA GCCAGCCTGG CAGGCGCGCC
    CCCGAGCCCG GAGGCCCCGA GCGAGAAGCC GAGCTCATCC CCAGGATGAA GGTGATTGAG
    AGCCCCCGGA GGTGCTCGGG GGGCGCGGCG GGCGCCGGCC GCGATGCGGA CACCAAGGAG
    GCGCGGGCCC CCCCGGAGGA CAGCCGCTCG CGGAGCAGCC CGGGCTCGCT GCCCCTGGCA
    AAGCCCAGGC CTGGGGCCAC CTCGCCTCTG AACTCTCCCG TGGCCGCCGC ATTACCTCCT
    CCAATCGGCA AGGAGCCCTG TCCCCCCAGC AGCCCCCTGC AGAAGGGGGG CTCCTTCTGG
    AGCTCCATCC CCGCCTCCCC CGCCAGCCGG CCAGGCTCCT TCACCTTCCC GGGGGACAGT
    GACTCCCTCC AGCGGCAGGC CCAGCGCCAC GCGGCCCCCA GCAAGGACAC AGACCGCATG
    AGCACCTGCT CGTCGGCCAG CGAGCAGTCG GTACAGTCCA CCCAGAGCAA CGGGAGTGAA
    AGTAGCAGCA GTAGCAACAT CTCCACCATG TTGGTGACAC ACGATTACAC GGCAGTGAAG
    GAGGATGAGA TAAACGTCTA TCAAGGAGAG GTCGTTCAAA TTCTGGCCAG CAATCAACAG
    AACATGTTTC TGGTGTTCCG AGCCGCCACT GACCAGTGCC CCGCAGCCGA GGGCTGGATT
    CCAGGCTTCG TCCTGGGGCA TACCAGTGCA GTCATCATGG AGAACCCCGA TGGGACTCTC
    AAGAAGTCAA CATCTTGGCA CACAGCACTC CGTTTAAGGA AAAAATCTGA GAAAAAAGAT
    AAAGACGGCA AGCGGGAAGG CAAGTTAGAG AATGGTTATC GGAAGTCACG GGAGGGGCTC
    AGCAGCAAGG TATCTGTGAA GCTTCTCAAT CCCAACTACA TATATGACGT TCCCCCAGAA
    TTCGTCATTC CCTTGAGTGA GGTCACGTGT GAGACAGGGG AGACCGTTGT TTTTAGATGT
    CGGGTTTGTG GCCGCCCCAA GGCCTCAATC ACCTGGAAGG GCCCTGAGCA CAACACCCTG
    AACAACGATG GTCACTACAG CATCTCCTAC AGTGACTTGG GAGAGGCCGC TTTGAAGATC
    GTCGGCGTGA CCACAGAAGA TGATGGCATC TACACATGCA TCGCTGTGAA CGACATGGGT
    TCAGCCTCGT CTTCGGCCAG TCTGAGGGTG CTGGGTCCAG GGAGTGATGG GATCATGGTG
    ACCTGGAAAG ACAACTTTGA CTCCTTCTAC AGCGAAGTGG CTGAGCTTGG CAGGGGCAGA
    TTCTCTGTCG TTAAGAAATG TGATCAGAAA GGAACCAAAC GAGCAGTGGC CACTAAATTT
    GTGAACAAGA AGTTGATGAA GCGTGACCAG GTCACCCATG AACTTGGAAT CCTGCAGAAC
    CTCCAGCACC CGCTGCTTAT TGGTCTCCTT GATACCTTCG AGACCCCCAC CAGCTACGTC
    CTGGTGTTAG AAATGGCTGA CCAGGGGCGT CTCCTGGACT GTGTGGTGCG ATGGGGAGAC
    CTCACTGAAG GGAAGATCAG GGCGTATCTG GGGGAGGTTC TGGAAGCCGT CCAATACCTT
    CACAACTGCA GGATAGCACA CCTGGACCTA AAGCCTGAAA ACATTCTGGT GGATCAGAGT
    CTGGCCAAGC CCACGATCAA ACTGGCCGAT TTTGGAGATG CTGTCCAACT CAACACGACC
    TACTACATCC ATCAGTTACT GGGGAACCCC GAGTTTGCAG CCCCCGAAAT CATCCTTGGG
    AACCCCATCT CCCTGACCTC GGATACGTGG AGTGTCGGAG TGCTCGCTTA CGTGCTTCTC
    AGCGGCGTGT CCCCCTTCCT CGACGACAGT GTGGAGGAGA CCTGCCTGAA CATTTGCCGA
    CTAGACTTTA GTTTCCCGGA GGACTGCTTT AAAGGTGTGA GCCAGAAGGC CAAGGAGTTC
    GTGTGTTTCC TTCTCCAGGA GGACCCTGCC AGGCGTCCCT CGGCTGCGCT GGCCCTGCAG
    GAGCAGTGGC TGCAGGCGGG CCACGGCGGT GGCAAAGGCG CGGGCGTCCT CGACACGTCC
    AGACTGACTT CCTTCATTGA GCGGCGCAAA CACCAGAACG ATGTTCGACC TATCCGTAGC
    GTTAAAAACT TCTTACAGAG CAGGCTTTTG CCTAGAGTTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe284505
    Clone ID Related Accession (Same CDS sequence) XM_022414356.1
    Accession Version XM_022414356.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6939bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product triple functional domain protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGAAAGCTA TGGATGTTTT GCCAATTTTA AAGGAAAAAG TTGCATACCT TTCAGGTGGC 
    AGAGATAAAC GTGGAGGTCC CATTTTAACA TTTCCTGCTC GCAGCAATCA TGATAGAATA
    CGACAGGAGG ACCTCAGGAG ACTCATTTCC TATCTAGCCT GTATCCCTAG TGAAGAGGTC
    TGCAAGCGAG GATTCACGGT GATCGTGGAC ATGCGTGGGT CCAAGTGGGA CTCCATTAAG
    CCTCTGCTGA AGATATTGCA AGAGTCCTTT CCCTGCTGCA TTCATGTTGC ACTGATAATC
    AAGCCAGACA ACTTTTGGCA GAAACAGAGG ACTAATTTTG GCAGTTCTAA GTTTGAATTT
    GAGACAAATA TGGTCTCTTT AGAAGGCCTT ACCAAAGTAG TTGATCCTTC TCAGCTAACT
    CCCGAGTTTG ATGGCTGCTT GGAATACAAC CATGAAGAAT GGATTGAAAT CAGAGTTGCT
    TTTGAAGACT ACATTAGCAA TGCAACCCAC ATGCTCTCTC GGCTGGAGGA ACTTCAGGAC
    ATCCTAGCCA AGAAAGAATT GCCTCAGGAT TTAGAGGGGG CTCGGAATAT GATTGAGGAA
    CATTCCCAGC TAAAGAAGAA GGTGATTAAG GCCCCTATAG AAGACTTAGA TTTAGAGGGA
    CAGAAGCTGC TCCAGAGGAT TCAGAGCAGT GACAGCTTTC CCAAAAAGAA CTCTGGGTCA
    GGCAATGCGG ACCTGCAGAG CCTCCTACCC AAGGTGTCCG CCATGTTGGA CAGGCTGCAC
    TCAACACGGC AGCATCTGCA TCAGATGTGG CATGTACGAA AACTGAAGCT GGACCAGTGC
    TTTCAGCTAA GGCTGTTTGA ACAGGACGCT GAAAAGATGT TTGACTGGAT CACACATAAC
    AAGGGCCTGT TTCTGAACAG CTACACTGAA ATTGGGACCA GCCATCCTCA TGCAATGGAA
    CTCCAGACAC AGCACAATCA CTTTGCCATG AACTGTATGA ACGTGTATGT TAATATAAAT
    CGCATAATGT CAGTGGCAAA TCGCTTGGTA GAGTCTGGCC ACTACGCCTC TCAGCAGATT
    AAGCAGATTG CGAATCAGCT GGAGCAGGAG TGGAAGGCAT TTGCGGCAGC CCTGGATGAG
    CGGAGTACCT TGCTGGACAT GTCCTCCATT TTCCACCAGA AGGCTGAAAA GTATATGAGC
    AACGTGGATT CGTGGTGTAA AGCCTGTGGT GAGGTAGACC TTCCCTCGGA GCTGCAGGAT
    CTGGAGGATG CCATTCACCA CCACCAGGGA ATATATGAGC ACATCACCCT TGCTTATTCC
    GAGGTGAGCC AGGATGGGAA GTCACTCCTC GACAAGCTCC AGCGGCCTTT GACTCCTGGC
    AGCTCCGACT CCCTCACGGC CTCTGCCAAC TACTCCAAGG CTGTGCACCA TGTCCTTGAT
    GTCATCCATG AGGTGCTGCA CCACCAGCGA CAGCTTGAGA ACATCTGGCA GCACCGCAAG
    GTCCGGCTCC ACCAGCGGCT GCAGCTGTGT GTGTTCCAGC AAGACGTTCA GCAGGTGCTG
    GACTGGATTG AGAACCATGG AGAAGCATTT CTGAGCAAAC ATACAGGTGT GGGGAAATCT
    CTTCACCGGG CCAGAGCTTT GCAGAAACGT CATGAAGATT TTGAAGAAGT AGCACAGAAC
    ACATATACCA ACGCAGATAA ATTACTAGAA GCAGCAGAAC AGCTGGCTCA GACCGGGGAA
    TGTGACCCAG AAGAGATTTA TCAGGCTGCG CATCAGCTTG AAGACCGGAT ACAAGATTTT
    GTTCGGCGCG TAGAGCAGCG CAAGATCCTG CTGGACATGT CAGTGTCGTT TCATAGCCAC
    GTCAAGGAGC TGTGGACATG GCTGGAGGAG CTGCAGAAGG AGCTGCTGGA CGATGTGTAC
    GCCGAGTCGG TGGAGGCCGT GCAGGACCTC ATCAAGCGCT TTGGCCAACA GCAGCAGACG
    ACCCTGCAAG TGACGGTCAA CGTGATCAAG GAAGGGGAGG ACCTCATACA GCAGCTGAGG
    GACTCAGCCA TCTCCAGCAA CAAGACCCCC CACAACAGCT CCATCAGTCA CATCGAGACG
    GTGCTTCAGC AGCTGGACGA GGCCCAGGCC CAGATGGAGG AGCTCTTCCA GGAGCGCAAA
    ATCAAGCTGG AGCTCTTCCT GCAGCTGCGC ATATTCGAGA GGGATGCCAT CGATATCATC
    TCAGACCTTG AGTCTTGGAA CGATGAGCTT TCTCAGCAAA TGAACGACTT TGACACAGAA
    GATCTCACAA TAGCAGAACA GCGCCTCCAG CACCATGCAG ACAAAGCTTT AACCATGAAC
    AACTTGACCT TTGATGTCAT CCACCAAGGG CAGGACCTCT TGCAGTATGT CAATGAAGTG
    CAGGCCTCTG GTGTGGAGCT GCTTTGCGAT AGAGATGTCG ACATGGCCAC TCGGGTCCAG
    GACCTCCTGG AGTTCCTTCA TGAAAAGCAG CAGGAACTGG ATTTAGCAGC AGAACAGCAT
    CGGAAGCACT TGGAGCAGTG TGTGCAGCTT CGCCATCTGC AGGCTGAAGT GAAGCAGGTG
    CTGGGCTGGA TCCGCAATGG AGAGTCAATG CTGAATGCTG GACTCATCAC GGCCAGCTCA
    TTACAGGAGG CGGAGCAGCT GCAGAGGGAG CACGAGCAGT TCCAGCACGC CATCGAGAAA
    ACACATCAAA GCGCCTTGCA GGTGCAGCAG AAGGCAGAAG CCATGCTGCA GGCCAATCAC
    TATGACATGG ACATGATCAG GGACTGCGCC GAGAAAGTGG CCTCTCACTG GCAGCAGCTC
    ATGCTCAAGA TGGAAGACCG CCTCAAGCTT GTGAATGCAT CTGTGGCCTT CTACAAAACC
    TCAGAGCAGG TCTGCAGTGT CCTCGAGAGC CTGGAGCAGG AGTACAAGAG AGAAGAGGAC
    TGGTGTGGAG GAGCCGACAA ACTGGGGCCC AACTCAGAGA CGGACCATGT CACCCCAATG
    ATCAGCAAGC ATCTGGAGCA GAAAGAAGCA TTCCTGAAGG CTTGCACGCT TGCTAGAAGG
    AATGCAGATG TCTTCCTGAA ATACCTGCAC AGGAATAGTG TGAACATGCC GGGAATGGTG
    ACACATATCA AAGCCCCTGA ACAACAAGTG AAAAATATCT TGAATGAACT TTTCCAACGG
    GAGAACAGGG TACTGCATTA TTGGACCATG AGAAAGAGAC GACTAGACCA GTGCCAGCAG
    TACGTGGTCT TTGAACGAAG TGCCAAGCAG GCTTTGGAAT GGATCCATGA CAATGGCGAG
    TTCTACCTCT CCACACACAC GTCCACGGGC TCCAGTATAC AGCACACCCA GGAGCTACTG
    AAAGAGCACG AGGAGTTTCA GATCACGGCA AAGCAAACCA AAGAGAGAGT GAAGCTATTG
    ATACAGCTGG CTGATGGCTT TTGTGAAAAA GGGCATGCCC ATGCAGCAGA GATAAAAAAA
    TGTGTCACTG CAGTGGATAA GAGGTACAGA GATTTCTCTC TGCGGATGGA GAAGTACAGG
    ACCTCTTTGG AGAAAGCCCT GGGGATTTCT TCAGATTCCA ACAAATCGAG CAAAAGTCTT
    CAGCTAGACA TAATCCCTGC CAGTGTCCCT GGGTCAGAGG TGAAGCTTCG TGATGCTGCT
    CATGAACTTA ATGAAGAGAA ACGGAAGTCG GCCCGCAGAA AAGAGTTCAT AATGGCTGAG
    CTGATTCAGA CTGAAAAGGC TTACGTGAGA GACCTCCGGG AGTGTATGGA TACATACCTG
    TGGGAAATGA CCAGCGGAGT GGAGGAGATC CCGGCCGGCA TCGTAAACAG AGAGCTTGTT
    ATCTTCGGGA ACATGCAGGA AATCTATGAG TTTCATAATA ACATATTCCT AAAAGAGCTG
    GAAAAATATG AGCAGTTGCC AGAAGATGTT GGACATTGCT TTGTTACTTG GGCAGACAAG
    TTTCAGATGT ATGTCACATA TTGCAAAAAT AAGCCTGATT CTACTCAGCT GATATTAGAG
    CATGCAGGAT CCTACTTTGA TGAAATACAA CAACGGCATG GATTAGCCAA TTCCATCTCT
    TCCTACCTTA TTAAGCCAGT TCAGCGAATA ACGAAGTATC AACTTCTTTT AAAAGAACTA
    CTTACTTGCT GTGAGGAAGG AAAGGGAGAG ATAAAAGATG GCCTGGAGGT GATGCTCAGT
    GTGCCAAAAC GAGCCAATGA TGCCATGCAC CTCAGCATGC TGGAAGGGTT TGATGAAAAT
    ATTGAGTCTC AGGGAGAACT CATTCTACAA GAATCCTTCC AAGTGTGGGA CCCAAAAACC
    TTAATTCGAA AGGGTCGAGA ACGGCATCTC TTCCTTTTTG AAATGTCCTT AGTATTTAGT
    AAAGAAGTGA AAGATTCCAG TGGCAGAAGC AAGTACCTTT ATAAAAGCAA ATTGTTTACC
    TCAGAGTTGG GTGTCACAGA ACACGTTGAA GGAGATCCTT GCAAATTTGC ACTGTGGGTG
    GGGAGGACAC CAACCTCAGA TAATAAAATC GTCCTTAAGG CTTCCAGTAT AGAAAACAAG
    CAGGACTGGA TAAAGCACAT CCGCGAGGTC ATACAGGAAA GGACCATTCA CCTGAAAGGA
    GCCCTGAAGG AGCCCATTCA CATTCCCAAA ACGGCGCCGG CCACCAGACA GAAGGGCAGG
    AGGGATGGAG AGGATCTGGA TAGCCAGGGC GATGGGAGCA GCCAGCCTGA CACAATCTCT
    ATCGCCTCGA GGACGTCTCA GAACACACTG GATAGTGATA AGCTCTCTGG CGGCTGCGAG
    CTGACAGTGG TGATCCACGA CTTTACCGCC TGCAACAGCA CAGAGCTGAC CATCCGCCGT
    GGCCAGACCG TGGAGGTTTT GGAGCGGCCC CACGACAAGC CTGACTGGTG TCTGGTGCGG
    ACCACCGACC GGTCCCCTGC TGCAGAAGGC CTGGTTCCCT GTGGCTCTCT CTGTATCGCC
    CACTCGAGAA GTAGCATGGA AATGGAGGGT ATCTTCAACC ACAAAGACTC GCTGTCCGTC
    TCCAGTAACG ATGCCAGCCC GCCGGCGTCC GTGGCCTCCC TCCAGCCCCA CCTGATGGGG
    GCCCAGAGCT CGCCAGGCCC CAAGCGCCCA GGGAACACTC TGCGCAAGTG GCTGACGAGC
    CCGGTGCGGC GGCTGAGCAG CGGGAAGGCG GACGGGCACG TGAAGAAGCT GGCGCACAAG
    CACAAGAAGA GCAGGGAGGT CCGCAAGAGC ACCGACGCGG GCTCGCAGAA GGACTCGGAC
    GACAGCGCGG CCACCCCGCA GGACGAGACT GTCGAGGAGA GAGGCCGGAA TGAAGGCCTG
    AGCAGTGGCA CACTCTCGAA GTCGTCATCG TCGGGCATGC AAAGCTGTGG AGAGGAAGAA
    GGGGAGGAGG GGGCCGACGC CGTGCCTCTC CCCCCGCCCA TGGCCATCCA GCAGCACAGC
    CTCCTCCAGC CGGATTCACA GGACGACAAG GCGTCTTCTC GGCTATTAGT TCGCCCCACC
    AGCTCTGAAA CGCCAAGCGC AGCCGAGCTC GTGAGTGCAA TTGAAGAACT CGTGAAAAGC
    AAGATGGCCT TGGAGGACCG TCCCAGCTCA CTCCTTGTTG ACCAGGGAGA CAGCAGCAGC
    CCCTCCTTCA ACCCTTCAGA CAACTCCCTC CTGTCCTCCT CCTCGCCCAT TGATGAGATG
    GAAGAAAGGA AATCCAGCTC TTTAAAGAGA CGGCACTATG TTTTGCAAGA ACTAGTGGAG
    ACAGAACGTG ACTATGTGCG AGACCTCGGC TATGTAGTTG AGGGCTACAT GGCTCTCATG
    AAAGAAGATG GTGTTCCTGA TGACATGAAA GGAAAGGACA AAATTGTATT TGGCAACATC
    CATCAGATTT ATGACTGGCA CAGAGACTTT TTTTTAGGAG AGTTGGAGAA GTGCCTTGAA
    GATCCAGAAA AACTAGGATC CCTTTTTGTT AAACACGAAA GAAGGTTGCA CATGTACATA
    GTTTATTGTC AAAATAAACC AAAGTCTGAG CACATTGTCT CAGAATACAT TGATACCTTT
    TTTGAGGACT TAAAGCAGCG TCTGGGCCAC AGGTTGCAGC TCACAGATCT GTTGATCAAA
    CCAGTGCAGC GGATTATGAA GTACCAGCTG CTCCTGAAGG ACTTCCTCAA GTATTCCAAA
    AAAGCTAGCC TGGATACATC AGAATTAGAG AGAGCTGTGG AAGTCATGTG CATAGTCCCC
    AAGCGGTGCA ACGACATGAT GAACGTGGGG CGGCTGCAAG GATTTGATGG TAAAATTGTT
    GCCCAGGGCA AGCTACTCTT GCAGGACACA TTCTTGGTCA CAGACCAGGA CTCGGGACTT
    CTGCCTCGCT GTAAAGAGAG GCGAGTTTTC CTCTTTGAGC AAATCGTCAT TTTCAGTGAA
    CCACTTGATA AAAAAAAGGG CTTCTCGATG CCAGGATTCC TGTTTAAGAA CAGTATCAAG
    GTGAGTTGCC TTTGCCTGGA GGAAAATGTG GAAAATGATC CATGTAAATT TGCTTTGACG
    TCGAGGACGG GTGGTGTGGT GGAGACCTTC ATTTTGCATT CATCCAGTCC AAGTGTCCGG
    CAAACATGGA TCCATGAAAT CAACCAAATT TTAGAAAACC AGCGCAATTT TTTAAATGCC
    TTGACATCAC CAATCGAGTA TCAGAGGAAC CACAGTGGGG GCGGCGGCGG GGGCAGTGGC
    GGGGGCGGCG GCAGCGGAGG CAGCGGCGGC GGGGCTGGCA GCGGGGCCCC CGGCGGCGGA
    GGCAGCAACC CCAGCGGCGG CCCTGGCAGC TGCAGCGGCC TCCCCAGCTC GAGCAGAAGC
    CGACCCTCCC GGATCCCCCA GCCTGTCAGA CACCACTCCC CCGTGCTGGT CTCCTCTGCA
    GCCTCGGGCC AGGCAGAGGC AGACAAGATG TCAGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022270064.1
    CDS21..6959
    Translation

    Target ORF information:

    RefSeq Version XM_022414356.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022414356.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGAAAGCTA TGGATGTTTT GCCAATTTTA AAGGAAAAAG TTGCATACCT TTCAGGTGGC 
    AGAGATAAAC GTGGAGGTCC CATTTTAACA TTTCCTGCTC GCAGCAATCA TGATAGAATA
    CGACAGGAGG ACCTCAGGAG ACTCATTTCC TATCTAGCCT GTATCCCTAG TGAAGAGGTC
    TGCAAGCGAG GATTCACGGT GATCGTGGAC ATGCGTGGGT CCAAGTGGGA CTCCATTAAG
    CCTCTGCTGA AGATATTGCA AGAGTCCTTT CCCTGCTGCA TTCATGTTGC ACTGATAATC
    AAGCCAGACA ACTTTTGGCA GAAACAGAGG ACTAATTTTG GCAGTTCTAA GTTTGAATTT
    GAGACAAATA TGGTCTCTTT AGAAGGCCTT ACCAAAGTAG TTGATCCTTC TCAGCTAACT
    CCCGAGTTTG ATGGCTGCTT GGAATACAAC CATGAAGAAT GGATTGAAAT CAGAGTTGCT
    TTTGAAGACT ACATTAGCAA TGCAACCCAC ATGCTCTCTC GGCTGGAGGA ACTTCAGGAC
    ATCCTAGCCA AGAAAGAATT GCCTCAGGAT TTAGAGGGGG CTCGGAATAT GATTGAGGAA
    CATTCCCAGC TAAAGAAGAA GGTGATTAAG GCCCCTATAG AAGACTTAGA TTTAGAGGGA
    CAGAAGCTGC TCCAGAGGAT TCAGAGCAGT GACAGCTTTC CCAAAAAGAA CTCTGGGTCA
    GGCAATGCGG ACCTGCAGAG CCTCCTACCC AAGGTGTCCG CCATGTTGGA CAGGCTGCAC
    TCAACACGGC AGCATCTGCA TCAGATGTGG CATGTACGAA AACTGAAGCT GGACCAGTGC
    TTTCAGCTAA GGCTGTTTGA ACAGGACGCT GAAAAGATGT TTGACTGGAT CACACATAAC
    AAGGGCCTGT TTCTGAACAG CTACACTGAA ATTGGGACCA GCCATCCTCA TGCAATGGAA
    CTCCAGACAC AGCACAATCA CTTTGCCATG AACTGTATGA ACGTGTATGT TAATATAAAT
    CGCATAATGT CAGTGGCAAA TCGCTTGGTA GAGTCTGGCC ACTACGCCTC TCAGCAGATT
    AAGCAGATTG CGAATCAGCT GGAGCAGGAG TGGAAGGCAT TTGCGGCAGC CCTGGATGAG
    CGGAGTACCT TGCTGGACAT GTCCTCCATT TTCCACCAGA AGGCTGAAAA GTATATGAGC
    AACGTGGATT CGTGGTGTAA AGCCTGTGGT GAGGTAGACC TTCCCTCGGA GCTGCAGGAT
    CTGGAGGATG CCATTCACCA CCACCAGGGA ATATATGAGC ACATCACCCT TGCTTATTCC
    GAGGTGAGCC AGGATGGGAA GTCACTCCTC GACAAGCTCC AGCGGCCTTT GACTCCTGGC
    AGCTCCGACT CCCTCACGGC CTCTGCCAAC TACTCCAAGG CTGTGCACCA TGTCCTTGAT
    GTCATCCATG AGGTGCTGCA CCACCAGCGA CAGCTTGAGA ACATCTGGCA GCACCGCAAG
    GTCCGGCTCC ACCAGCGGCT GCAGCTGTGT GTGTTCCAGC AAGACGTTCA GCAGGTGCTG
    GACTGGATTG AGAACCATGG AGAAGCATTT CTGAGCAAAC ATACAGGTGT GGGGAAATCT
    CTTCACCGGG CCAGAGCTTT GCAGAAACGT CATGAAGATT TTGAAGAAGT AGCACAGAAC
    ACATATACCA ACGCAGATAA ATTACTAGAA GCAGCAGAAC AGCTGGCTCA GACCGGGGAA
    TGTGACCCAG AAGAGATTTA TCAGGCTGCG CATCAGCTTG AAGACCGGAT ACAAGATTTT
    GTTCGGCGCG TAGAGCAGCG CAAGATCCTG CTGGACATGT CAGTGTCGTT TCATAGCCAC
    GTCAAGGAGC TGTGGACATG GCTGGAGGAG CTGCAGAAGG AGCTGCTGGA CGATGTGTAC
    GCCGAGTCGG TGGAGGCCGT GCAGGACCTC ATCAAGCGCT TTGGCCAACA GCAGCAGACG
    ACCCTGCAAG TGACGGTCAA CGTGATCAAG GAAGGGGAGG ACCTCATACA GCAGCTGAGG
    GACTCAGCCA TCTCCAGCAA CAAGACCCCC CACAACAGCT CCATCAGTCA CATCGAGACG
    GTGCTTCAGC AGCTGGACGA GGCCCAGGCC CAGATGGAGG AGCTCTTCCA GGAGCGCAAA
    ATCAAGCTGG AGCTCTTCCT GCAGCTGCGC ATATTCGAGA GGGATGCCAT CGATATCATC
    TCAGACCTTG AGTCTTGGAA CGATGAGCTT TCTCAGCAAA TGAACGACTT TGACACAGAA
    GATCTCACAA TAGCAGAACA GCGCCTCCAG CACCATGCAG ACAAAGCTTT AACCATGAAC
    AACTTGACCT TTGATGTCAT CCACCAAGGG CAGGACCTCT TGCAGTATGT CAATGAAGTG
    CAGGCCTCTG GTGTGGAGCT GCTTTGCGAT AGAGATGTCG ACATGGCCAC TCGGGTCCAG
    GACCTCCTGG AGTTCCTTCA TGAAAAGCAG CAGGAACTGG ATTTAGCAGC AGAACAGCAT
    CGGAAGCACT TGGAGCAGTG TGTGCAGCTT CGCCATCTGC AGGCTGAAGT GAAGCAGGTG
    CTGGGCTGGA TCCGCAATGG AGAGTCAATG CTGAATGCTG GACTCATCAC GGCCAGCTCA
    TTACAGGAGG CGGAGCAGCT GCAGAGGGAG CACGAGCAGT TCCAGCACGC CATCGAGAAA
    ACACATCAAA GCGCCTTGCA GGTGCAGCAG AAGGCAGAAG CCATGCTGCA GGCCAATCAC
    TATGACATGG ACATGATCAG GGACTGCGCC GAGAAAGTGG CCTCTCACTG GCAGCAGCTC
    ATGCTCAAGA TGGAAGACCG CCTCAAGCTT GTGAATGCAT CTGTGGCCTT CTACAAAACC
    TCAGAGCAGG TCTGCAGTGT CCTCGAGAGC CTGGAGCAGG AGTACAAGAG AGAAGAGGAC
    TGGTGTGGAG GAGCCGACAA ACTGGGGCCC AACTCAGAGA CGGACCATGT CACCCCAATG
    ATCAGCAAGC ATCTGGAGCA GAAAGAAGCA TTCCTGAAGG CTTGCACGCT TGCTAGAAGG
    AATGCAGATG TCTTCCTGAA ATACCTGCAC AGGAATAGTG TGAACATGCC GGGAATGGTG
    ACACATATCA AAGCCCCTGA ACAACAAGTG AAAAATATCT TGAATGAACT TTTCCAACGG
    GAGAACAGGG TACTGCATTA TTGGACCATG AGAAAGAGAC GACTAGACCA GTGCCAGCAG
    TACGTGGTCT TTGAACGAAG TGCCAAGCAG GCTTTGGAAT GGATCCATGA CAATGGCGAG
    TTCTACCTCT CCACACACAC GTCCACGGGC TCCAGTATAC AGCACACCCA GGAGCTACTG
    AAAGAGCACG AGGAGTTTCA GATCACGGCA AAGCAAACCA AAGAGAGAGT GAAGCTATTG
    ATACAGCTGG CTGATGGCTT TTGTGAAAAA GGGCATGCCC ATGCAGCAGA GATAAAAAAA
    TGTGTCACTG CAGTGGATAA GAGGTACAGA GATTTCTCTC TGCGGATGGA GAAGTACAGG
    ACCTCTTTGG AGAAAGCCCT GGGGATTTCT TCAGATTCCA ACAAATCGAG CAAAAGTCTT
    CAGCTAGACA TAATCCCTGC CAGTGTCCCT GGGTCAGAGG TGAAGCTTCG TGATGCTGCT
    CATGAACTTA ATGAAGAGAA ACGGAAGTCG GCCCGCAGAA AAGAGTTCAT AATGGCTGAG
    CTGATTCAGA CTGAAAAGGC TTACGTGAGA GACCTCCGGG AGTGTATGGA TACATACCTG
    TGGGAAATGA CCAGCGGAGT GGAGGAGATC CCGGCCGGCA TCGTAAACAG AGAGCTTGTT
    ATCTTCGGGA ACATGCAGGA AATCTATGAG TTTCATAATA ACATATTCCT AAAAGAGCTG
    GAAAAATATG AGCAGTTGCC AGAAGATGTT GGACATTGCT TTGTTACTTG GGCAGACAAG
    TTTCAGATGT ATGTCACATA TTGCAAAAAT AAGCCTGATT CTACTCAGCT GATATTAGAG
    CATGCAGGAT CCTACTTTGA TGAAATACAA CAACGGCATG GATTAGCCAA TTCCATCTCT
    TCCTACCTTA TTAAGCCAGT TCAGCGAATA ACGAAGTATC AACTTCTTTT AAAAGAACTA
    CTTACTTGCT GTGAGGAAGG AAAGGGAGAG ATAAAAGATG GCCTGGAGGT GATGCTCAGT
    GTGCCAAAAC GAGCCAATGA TGCCATGCAC CTCAGCATGC TGGAAGGGTT TGATGAAAAT
    ATTGAGTCTC AGGGAGAACT CATTCTACAA GAATCCTTCC AAGTGTGGGA CCCAAAAACC
    TTAATTCGAA AGGGTCGAGA ACGGCATCTC TTCCTTTTTG AAATGTCCTT AGTATTTAGT
    AAAGAAGTGA AAGATTCCAG TGGCAGAAGC AAGTACCTTT ATAAAAGCAA ATTGTTTACC
    TCAGAGTTGG GTGTCACAGA ACACGTTGAA GGAGATCCTT GCAAATTTGC ACTGTGGGTG
    GGGAGGACAC CAACCTCAGA TAATAAAATC GTCCTTAAGG CTTCCAGTAT AGAAAACAAG
    CAGGACTGGA TAAAGCACAT CCGCGAGGTC ATACAGGAAA GGACCATTCA CCTGAAAGGA
    GCCCTGAAGG AGCCCATTCA CATTCCCAAA ACGGCGCCGG CCACCAGACA GAAGGGCAGG
    AGGGATGGAG AGGATCTGGA TAGCCAGGGC GATGGGAGCA GCCAGCCTGA CACAATCTCT
    ATCGCCTCGA GGACGTCTCA GAACACACTG GATAGTGATA AGCTCTCTGG CGGCTGCGAG
    CTGACAGTGG TGATCCACGA CTTTACCGCC TGCAACAGCA CAGAGCTGAC CATCCGCCGT
    GGCCAGACCG TGGAGGTTTT GGAGCGGCCC CACGACAAGC CTGACTGGTG TCTGGTGCGG
    ACCACCGACC GGTCCCCTGC TGCAGAAGGC CTGGTTCCCT GTGGCTCTCT CTGTATCGCC
    CACTCGAGAA GTAGCATGGA AATGGAGGGT ATCTTCAACC ACAAAGACTC GCTGTCCGTC
    TCCAGTAACG ATGCCAGCCC GCCGGCGTCC GTGGCCTCCC TCCAGCCCCA CCTGATGGGG
    GCCCAGAGCT CGCCAGGCCC CAAGCGCCCA GGGAACACTC TGCGCAAGTG GCTGACGAGC
    CCGGTGCGGC GGCTGAGCAG CGGGAAGGCG GACGGGCACG TGAAGAAGCT GGCGCACAAG
    CACAAGAAGA GCAGGGAGGT CCGCAAGAGC ACCGACGCGG GCTCGCAGAA GGACTCGGAC
    GACAGCGCGG CCACCCCGCA GGACGAGACT GTCGAGGAGA GAGGCCGGAA TGAAGGCCTG
    AGCAGTGGCA CACTCTCGAA GTCGTCATCG TCGGGCATGC AAAGCTGTGG AGAGGAAGAA
    GGGGAGGAGG GGGCCGACGC CGTGCCTCTC CCCCCGCCCA TGGCCATCCA GCAGCACAGC
    CTCCTCCAGC CGGATTCACA GGACGACAAG GCGTCTTCTC GGCTATTAGT TCGCCCCACC
    AGCTCTGAAA CGCCAAGCGC AGCCGAGCTC GTGAGTGCAA TTGAAGAACT CGTGAAAAGC
    AAGATGGCCT TGGAGGACCG TCCCAGCTCA CTCCTTGTTG ACCAGGGAGA CAGCAGCAGC
    CCCTCCTTCA ACCCTTCAGA CAACTCCCTC CTGTCCTCCT CCTCGCCCAT TGATGAGATG
    GAAGAAAGGA AATCCAGCTC TTTAAAGAGA CGGCACTATG TTTTGCAAGA ACTAGTGGAG
    ACAGAACGTG ACTATGTGCG AGACCTCGGC TATGTAGTTG AGGGCTACAT GGCTCTCATG
    AAAGAAGATG GTGTTCCTGA TGACATGAAA GGAAAGGACA AAATTGTATT TGGCAACATC
    CATCAGATTT ATGACTGGCA CAGAGACTTT TTTTTAGGAG AGTTGGAGAA GTGCCTTGAA
    GATCCAGAAA AACTAGGATC CCTTTTTGTT AAACACGAAA GAAGGTTGCA CATGTACATA
    GTTTATTGTC AAAATAAACC AAAGTCTGAG CACATTGTCT CAGAATACAT TGATACCTTT
    TTTGAGGACT TAAAGCAGCG TCTGGGCCAC AGGTTGCAGC TCACAGATCT GTTGATCAAA
    CCAGTGCAGC GGATTATGAA GTACCAGCTG CTCCTGAAGG ACTTCCTCAA GTATTCCAAA
    AAAGCTAGCC TGGATACATC AGAATTAGAG AGAGCTGTGG AAGTCATGTG CATAGTCCCC
    AAGCGGTGCA ACGACATGAT GAACGTGGGG CGGCTGCAAG GATTTGATGG TAAAATTGTT
    GCCCAGGGCA AGCTACTCTT GCAGGACACA TTCTTGGTCA CAGACCAGGA CTCGGGACTT
    CTGCCTCGCT GTAAAGAGAG GCGAGTTTTC CTCTTTGAGC AAATCGTCAT TTTCAGTGAA
    CCACTTGATA AAAAAAAGGG CTTCTCGATG CCAGGATTCC TGTTTAAGAA CAGTATCAAG
    GTGAGTTGCC TTTGCCTGGA GGAAAATGTG GAAAATGATC CATGTAAATT TGCTTTGACG
    TCGAGGACGG GTGGTGTGGT GGAGACCTTC ATTTTGCATT CATCCAGTCC AAGTGTCCGG
    CAAACATGGA TCCATGAAAT CAACCAAATT TTAGAAAACC AGCGCAATTT TTTAAATGCC
    TTGACATCAC CAATCGAGTA TCAGAGGAAC CACAGTGGGG GCGGCGGCGG GGGCAGTGGC
    GGGGGCGGCG GCAGCGGAGG CAGCGGCGGC GGGGCTGGCA GCGGGGCCCC CGGCGGCGGA
    GGCAGCAACC CCAGCGGCGG CCCTGGCAGC TGCAGCGGCC TCCCCAGCTC GAGCAGAAGC
    CGACCCTCCC GGATCCCCCA GCCTGTCAGA CACCACTCCC CCGTGCTGGT CTCCTCTGCA
    GCCTCGGGCC AGGCAGAGGC AGACAAGATG TCAGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44775
    Clone ID Related Accession (Same CDS sequence) XM_014110313.1
    Accession Version XM_014110313.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9369bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product triple functional domain protein isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726119.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1321
    1381
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    1501
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    9001
    9061
    9121
    9181
    9241
    9301
    9361
    ATGAAGGAAT TCTTCATTTC CCTACTTTTT CTAAACCATT GTTCCCTGAA ACTGCTGCTT 
    TTAGGGCAGA GATTTAATCT GTCCTTTATT GCTCTATATT TGCCAACAGG AGCAGTGAGT
    TGTATGTGTG GTGGTGCTAA GACCACACAG CGAAGGCTTT CTTGTGCTGC CAGAGATCGA
    AAGGGAGGGT TTCGAAAAAA CGATGAAATG AAAGCTATGG ATGTTTTGCC AATTTTAAAG
    GAAAAAGTTG CATACCTTTC AGGTGGCAGA GATAAACGTG GAGGTCCCAT TTTAACATTT
    CCTGCTCGCA GCAATCATGA TAGAATACGA CAGGAGGACC TCAGGAGACT CATTTCCTAT
    CTAGCCTGTA TCCCTAGTGA AGAGGTCTGC AAGCGAGGAT TCACGGTGAT CGTGGACATG
    CGTGGGTCCA AGTGGGACTC CATTAAGCCT CTGCTGAAGA TATTGCAAGA GTCCTTTCCC
    TGCTGCATTC ATGTTGCACT GATAATCAAG CCAGACAACT TTTGGCAGAA ACAGAGGACT
    AATTTTGGCA GTTCTAAGTT TGAATTTGAG ACAAATATGG TCTCTTTAGA AGGCCTTACC
    AAAGTAGTTG ATCCTTCTCA GCTAACTCCC GAGTTTGATG GCTGCTTGGA ATACAACCAT
    GAAGAATGGA TTGAAATCAG AGTTGCTTTT GAAGACTACA TTAGCAATGC AACCCACATG
    CTCTCTCGGC TGGAGGAACT TCAGGACATC CTAGCCAAGA AAGAATTGCC TCAGGATTTA
    GAGGGGGCTC GGAATATGAT TGAGGAACAT TCCCAGCTAA AGAAGAAGGT GATTAAGGCC
    CCTATAGAAG ACTTAGATTT AGAGGGACAG AAGCTGCTCC AGAGGATTCA GAGCAGTGAC
    AGCTTTCCCA AAAAGAACTC TGGGTCAGGC AATGCGGACC TGCAGAGCCT CCTACCCAAG
    GTGTCCGCCA TGTTGGACAG GCTGCACTCA ACACGGCAGC ATCTGCATCA GATGTGGCAT
    GTACGAAAAC TGAAGCTGGA CCAGTGCTTT CAGCTAAGGC TGTTTGAACA GGACGCTGAA
    AAGATGTTTG ACTGGATCAC ACATAACAAG GGCCTGTTTC TGAACAGCTA CACTGAAATT
    GGGACCAGCC ATCCTCATGC AATGGAACTC CAGACACAGC ACAATCACTT TGCCATGAAC
    TGTATGAACG TGTATGTTAA TATAAATCGC ATAATGTCAG TGGCAAATCG CTTGGTAGAG
    TCTGGCCACT ACGCCTCTCA GCAGATTAAG CAGATTGCGA ATCAGCTGGA GCAGGAGTGG
    AAGGCATTTG CGGCAGCCCT GGATGAGCGG AGTACCTTGC TGGACATGTC CTCCATTTTC
    CACCAGAAGG CTGAAAAGTA TATGAGCAAC GTGGATTCGT GGTGTAAAGC CTGTGGTGAG
    GTAGACCTTC CCTCGGAGCT GCAGGATCTG GAGGATGCCA TTCACCACCA CCAGGGAATA
    TATGAGCACA TCACCCTTGC TTATTCCGAG GTGAGCCAGG ATGGGAAGTC ACTCCTCGAC
    AAGCTCCAGC GGCCTTTGAC TCCTGGCAGC TCCGACTCCC TCACGGCCTC TGCCAACTAC
    TCCAAGGCTG TGCACCATGT CCTTGATGTC ATCCATGAGG TGCTGCACCA CCAGCGACAG
    CTTGAGAACA TCTGGCAGCA CCGCAAGGTC CGGCTCCACC AGCGGCTGCA GCTGTGTGTG
    TTCCAGCAAG ACGTTCAGCA GGTGCTGGAC TGGATTGAGA ACCATGGAGA AGCATTTCTG
    AGCAAACATA CAGGTGTGGG GAAATCTCTT CACCGGGCCA GAGCTTTGCA GAAACGTCAT
    GAAGATTTTG AAGAAGTAGC ACAGAACACA TATACCAACG CAGATAAATT ACTAGAAGCA
    GCAGAACAGC TGGCTCAGAC CGGGGAATGT GACCCAGAAG AGATTTATCA GGCTGCGCAT
    CAGCTTGAAG ACCGGATACA AGATTTTGTT CGGCGCGTAG AGCAGCGCAA GATCCTGCTG
    GACATGTCAG TGTCGTTTCA TAGCCACGTC AAGGAGCTGT GGACATGGCT GGAGGAGCTG
    CAGAAGGAGC TGCTGGACGA TGTGTACGCC GAGTCGGTGG AGGCCGTGCA GGACCTCATC
    AAGCGCTTTG GCCAACAGCA GCAGACGACC CTGCAAGTGA CGGTCAACGT GATCAAGGAA
    GGGGAGGACC TCATACAGCA GCTGAGGGAC TCAGCCATCT CCAGCAACAA GACCCCCCAC
    AACAGCTCCA TCAGTCACAT CGAGACGGTG CTTCAGCAGC TGGACGAGGC CCAGGCCCAG
    ATGGAGGAGC TCTTCCAGGA GCGCAAAATC AAGCTGGAGC TCTTCCTGCA GCTGCGCATA
    TTCGAGAGGG ATGCCATCGA TATCATCTCA GACCTTGAGT CTTGGAACGA TGAGCTTTCT
    CAGCAAATGA ACGACTTTGA CACAGAAGAT CTCACAATAG CAGAACAGCG CCTCCAGCAC
    CATGCAGACA AAGCTTTAAC CATGAACAAC TTGACCTTTG ATGTCATCCA CCAAGGGCAG
    GACCTCTTGC AGTATGTCAA TGAAGTGCAG GCCTCTGGTG TGGAGCTGCT TTGCGATAGA
    GATGTCGACA TGGCCACTCG GGTCCAGGAC CTCCTGGAGT TCCTTCATGA AAAGCAGCAG
    GAACTGGATT TAGCAGCAGA ACAGCATCGG AAGCACTTGG AGCAGTGTGT GCAGCTTCGC
    CATCTGCAGG CTGAAGTGAA GCAGGTGCTG GGCTGGATCC GCAATGGAGA GTCAATGCTG
    AATGCTGGAC TCATCACGGC CAGCTCATTA CAGGAGGCGG AGCAGCTGCA GAGGGAGCAC
    GAGCAGTTCC AGCACGCCAT CGAGAAAACA CATCAAAGCG CCTTGCAGGT GCAGCAGAAG
    GCAGAAGCCA TGCTGCAGGC CAATCACTAT GACATGGACA TGATCAGGGA CTGCGCCGAG
    AAAGTGGCCT CTCACTGGCA GCAGCTCATG CTCAAGATGG AAGACCGCCT CAAGCTTGTG
    AATGCATCTG TGGCCTTCTA CAAAACCTCA GAGCAGGTCT GCAGTGTCCT CGAGAGCCTG
    GAGCAGGAGT ACAAGAGAGA AGAGGACTGG TGTGGAGGAG CCGACAAACT GGGGCCCAAC
    TCAGAGACGG ACCATGTCAC CCCAATGATC AGCAAGCATC TGGAGCAGAA AGAAGCATTC
    CTGAAGGCTT GCACGCTTGC TAGAAGGAAT GCAGATGTCT TCCTGAAATA CCTGCACAGG
    AATAGTGTGA ACATGCCGGG AATGGTGACA CATATCAAAG CCCCTGAACA ACAAGTGAAA
    AATATCTTGA ATGAACTTTT CCAACGGGAG AACAGGGTAC TGCATTATTG GACCATGAGA
    AAGAGACGAC TAGACCAGTG CCAGCAGTAC GTGGTCTTTG AACGAAGTGC CAAGCAGGCT
    TTGGAATGGA TCCATGACAA TGGCGAGTTC TACCTCTCCA CACACACGTC CACGGGCTCC
    AGTATACAGC ACACCCAGGA GCTACTGAAA GAGCACGAGG AGTTTCAGAT CACGGCAAAG
    CAAACCAAAG AGAGAGTGAA GCTATTGATA CAGCTGGCTG ATGGCTTTTG TGAAAAAGGG
    CATGCCCATG CAGCAGAGAT AAAAAAATGT GTCACTGCAG TGGATAAGAG GTACAGAGAT
    TTCTCTCTGC GGATGGAGAA GTACAGGACC TCTTTGGAGA AAGCCCTGGG GATTTCTTCA
    GATTCCAACA AATCGAGCAA AAGTCTTCAG CTAGACATAA TCCCTGCCAG TGTCCCTGGG
    TCAGAGGTGA AGCTTCGTGA TGCTGCTCAT GAACTTAATG AAGAGAAACG GAAGTCGGCC
    CGCAGAAAAG AGTTCATAAT GGCTGAGCTG ATTCAGACTG AAAAGGCTTA CGTGAGAGAC
    CTCCGGGAGT GTATGGATAC ATACCTGTGG GAAATGACCA GCGGAGTGGA GGAGATCCCG
    GCCGGCATCG TAAACAGAGA GCTTGTTATC TTCGGGAACA TGCAGGAAAT CTATGAGTTT
    CATAATAACA TATTCCTAAA AGAGCTGGAA AAATATGAGC AGTTGCCAGA AGATGTTGGA
    CATTGCTTTG TTACTTGGGC AGACAAGTTT CAGATGTATG TCACATATTG CAAAAATAAG
    CCTGATTCTA CTCAGCTGAT ATTAGAGCAT GCAGGATCCT ACTTTGATGA AATACAACAA
    CGGCATGGAT TAGCCAATTC CATCTCTTCC TACCTTATTA AGCCAGTTCA GCGAATAACG
    AAGTATCAAC TTCTTTTAAA AGAACTACTT ACTTGCTGTG AGGAAGGAAA GGGAGAGATA
    AAAGATGGCC TGGAGGTGAT GCTCAGTGTG CCAAAACGAG CCAATGATGC CATGCACCTC
    AGCATGCTGG AAGGGTTTGA TGAAAATATT GAGTCTCAGG GAGAACTCAT TCTACAAGAA
    TCCTTCCAAG TGTGGGACCC AAAAACCTTA ATTCGAAAGG GTCGAGAACG GCATCTCTTC
    CTTTTTGAAA TGTCCTTAGT ATTTAGTAAA GAAGTGAAAG ATTCCAGTGG CAGAAGCAAG
    TACCTTTATA AAAGCAAATT GTTTACCTCA GAGTTGGGTG TCACAGAACA CGTTGAAGGA
    GATCCTTGCA AATTTGCACT GTGGGTGGGG AGGACACCAA CCTCAGATAA TAAAATCGTC
    CTTAAGGCTT CCAGTATAGA AAACAAGCAG GACTGGATAA AGCACATCCG CGAGGTCATA
    CAGGAAAGGA CCATTCACCT GAAAGGAGCC CTGAAGGAGC CCATTCACAT TCCCAAAACG
    GCGCCGGCCA CCAGACAGAA GGGCAGGAGG GATGGAGAGG ATCTGGATAG CCAGGGCGAT
    GGGAGCAGCC AGCCTGACAC AATCTCTATC GCCTCGAGGA CGTCTCAGAA CACACTGGAT
    AGTGATAAGC TCTCTGGCGG CTGCGAGCTG ACAGTGGTGA TCCACGACTT TACCGCCTGC
    AACAGCACAG AGCTGACCAT CCGCCGTGGC CAGACCGTGG AGGTTTTGGA GCGGCCCCAC
    GACAAGCCTG ACTGGTGTCT GGTGCGGACC ACCGACCGGT CCCCTGCTGC AGAAGGCCTG
    GTTCCCTGTG GCTCTCTCTG TATCGCCCAC TCGAGAAGTA GCATGGAAAT GGAGGGTATC
    TTCAACCACA AAGACTCGCT GTCCGTCTCC AGTAACGATG CCAGCCCGCC GGCGTCCGTG
    GCCTCCCTCC AGCCCCACCT GATGGGGGCC CAGAGCTCGC CAGGCCCCAA GCGCCCAGGG
    AACACTCTGC GCAAGTGGCT GACGAGCCCG GTGCGGCGGC TGAGCAGCGG GAAGGCGGAC
    GGGCACGTGA AGAAGCTGGC GCACAAGCAC AAGAAGAGCA GGGAGGTCCG CAAGAGCACC
    GACGCGGGCT CGCAGAAGGA CTCGGACGAC AGCGCGGCCA CCCCGCAGGA CGAGACTGTC
    GAGGAGAGAG GCCGGAATGA AGGCCTGAGC AGTGGCACAC TCTCGAAGTC GTCATCGTCG
    GGCATGCAAA GCTGTGGAGA GGAAGAAGGG GAGGAGGGGG CCGACGCCGT GCCTCTCCCC
    CCGCCCATGG CCATCCAGCA GCACAGCCTC CTCCAGCCGG ATTCACAGGA CGACAAGGCG
    TCTTCTCGGC TATTAGTTCG CCCCACCAGC TCTGAAACGC CAAGCGCAGC CGAGCTCGTG
    AGTGCAATTG AAGAACTCGT GAAAAGCAAG ATGGCCTTGG AGGACCGTCC CAGCTCACTC
    CTTGTTGACC AGGGAGACAG CAGCAGCCCC TCCTTCAACC CTTCAGACAA CTCCCTCCTG
    TCCTCCTCCT CGCCCATTGA TGAGATGGAA GAAAGGAAAT CCAGCTCTTT AAAGAGACGG
    CACTATGTTT TGCAAGAACT AGTGGAGACA GAACGTGACT ATGTGCGAGA CCTCGGCTAT
    GTAGTTGAGG GCTACATGGC TCTCATGAAA GAAGATGGTG TTCCTGATGA CATGAAAGGA
    AAGGACAAAA TTGTATTTGG CAACATCCAT CAGATTTATG ACTGGCACAG AGACTTTTTT
    TTAGGAGAGT TGGAGAAGTG CCTTGAAGAT CCAGAAAAAC TAGGATCCCT TTTTGTTAAA
    CACGAAAGAA GGTTGCACAT GTACATAGTT TATTGTCAAA ATAAACCAAA GTCTGAGCAC
    ATTGTCTCAG AATACATTGA TACCTTTTTT GAGGACTTAA AGCAGCGTCT GGGCCACAGG
    TTGCAGCTCA CAGATCTGTT GATCAAACCA GTGCAGCGGA TTATGAAGTA CCAGCTGCTC
    CTGAAGGACT TCCTCAAGTA TTCCAAAAAA GCTAGCCTGG ATACATCAGA ATTAGAGAGA
    GCTGTGGAAG TCATGTGCAT AGTCCCCAAG CGGTGCAACG ACATGATGAA CGTGGGGCGG
    CTGCAAGGAT TTGATGGTAA AATTGTTGCC CAGGGCAAGC TACTCTTGCA GGACACATTC
    TTGGTCACAG ACCAGGACTC GGGACTTCTG CCTCGCTGTA AAGAGAGGCG AGTTTTCCTC
    TTTGAGCAAA TCGTCATTTT CAGTGAACCA CTTGATAAAA AAAAGGGCTT CTCGATGCCA
    GGATTCCTGT TTAAGAACAG TATCAAGGTG AGTTGCCTTT GCCTGGAGGA AAATGTGGAA
    AATGATCCAT GTAAATTTGC TTTGACGTCG AGGACGGGTG GTGTGGTGGA GACCTTCATT
    TTGCATTCAT CCAGTCCAAG TGTCCGGCAA ACATGGATCC ATGAAATCAA CCAAATTTTA
    GAAAACCAGC GCAATTTTTT AAATGCCTTG ACATCACCAA TCGAGTATCA GAGGAACCAC
    AGTGGGGGCG GCGGCGGGGG CAGTGGCGGG GGCGGCGGCA GCGGAGGCAG CGGCGGCGGG
    GCTGGCAGCG GGGCCCCCGG CGGCGGAGGC AGCAACCCCA GCGGCGGCCC TGGCAGCTGC
    AGCGGCCTCC CCAGCTCGAG CAGAAGCCGA CCCTCCCGGA TCCCCCAGCC TGTCAGACAC
    CACTCCCCCG TGCTGGTCTC CTCTGCAGCC TCGGGCCAGG CAGAGGCAGA CAAGATGTCA
    GGTACGTCCA CTCCCGGCCC TGCACTGGCC CCTCCCGGCA GCAGCCCCGC CCCGGAGCCC
    GGCCCCAGCC AGCCTGGCAG GCGCGCCCCC GAGCCCGGAG GCCCCGAGCG AGAAGCCGAG
    CTCATCCCCA GGATGAAGGT GATTGAGAGC CCCCGGAGGT GCTCGGGGGG CGCGGCGGGC
    GCCGGCCGCG ATGCGGACAC CAAGGAGGCG CGGGCCCCCC CGGAGGACAG CCGCTCGCGG
    AGCAGCCCGG GCTCGCTGCC CCTGGCAAAG CCCAGGCCTG GGGCCACCTC GCCTCTGAAC
    TCTCCCGTGG CCGCCGCATT ACCTCCTCCA ATCGGCAAGG AGCCCTGTCC CCCCAGCAGC
    CCCCTGCAGA AGGGGGGCTC CTTCTGGAGC TCCATCCCCG CCTCCCCCGC CAGCCGGCCA
    GGCTCCTTCA CCTTCCCGGG GGACAGTGAC TCCCTCCAGC GGCAGGCCCA GCGCCACGCG
    GCCCCCAGCA AGGACACAGA CCGCATGAGC ACCTGCTCGT CGGCCAGCGA GCAGTCGGTA
    CAGTCCACCC AGAGCAACGG GAGTGAAAGT AGCAGCAGTA GCAACATCTC CACCATGTTG
    GTGACACACG ATTACACGGC AGTGAAGGAG GATGAGATAA ACGTCTATCA AGGAGAGGTC
    GTTCAAATTC TGGCCAGCAA TCAACAGAAC ATGTTTCTGG TGTTCCGAGC CGCCACTGAC
    CAGTGCCCCG CAGCCGAGGG CTGGATTCCA GGCTTCGTCC TGGGGCATAC CAGTGCAGTC
    ATCATGGAGA ACCCCGATGG GACTCTCAAG AAGTCAACAT CTTGGCACAC AGCACTCCGT
    TTAAGGAAAA AATCTGAGAA AAAAGATAAA GACGGCAAGC GGGAAGGCAA GTTAGAGAAT
    GGTTATCGGA AGTCACGGGA GGGGCTCAGC AGCAAGGTAT CTGTGAAGCT TCTCAATCCC
    AACTACATAT ATGACGTTCC CCCAGAATTC GTCATTCCCT TGAGTGAGGT CACGTGTGAG
    ACAGGGGAGA CCGTTGTTTT TAGATGTCGG GTTTGTGGCC GCCCCAAGGC CTCAATCACC
    TGGAAGGGCC CTGAGCACAA CACCCTGAAC AACGATGGTC ACTACAGCAT CTCCTACAGT
    GACTTGGGAG AGGCCGCTTT GAAGATCGTC GGCGTGACCA CAGAAGATGA TGGCATCTAC
    ACATGCATCG CTGTGAACGA CATGGGTTCA GCCTCGTCTT CGGCCAGTCT GAGGGTGCTG
    GGTCCAGGGA GTGATGGGAT CATGGTGACC TGGAAAGACA ACTTTGACTC CTTCTACAGC
    GAAGTGGCTG AGCTTGGCAG GGGCAGATTC TCTGTCGTTA AGAAATGTGA TCAGAAAGGA
    ACCAAACGAG CAGTGGCCAC TAAATTTGTG AACAAGAAGT TGATGAAGCG TGACCAGGTC
    ACCCATGAAC TTGGAATCCT GCAGAACCTC CAGCACCCGC TGCTTATTGG TCTCCTTGAT
    ACCTTCGAGA CCCCCACCAG CTACGTCCTG GTGTTAGAAA TGGCTGACCA GGGGCGTCTC
    CTGGACTGTG TGGTGCGATG GGGAGACCTC ACTGAAGGGA AGATCAGGGC GTATCTGGGG
    GAGGTTCTGG AAGCCGTCCA ATACCTTCAC AACTGCAGGA TAGCACACCT GGACCTAAAG
    CCTGAAAACA TTCTGGTGGA TCAGAGTCTG GCCAAGCCCA CGATCAAACT GGCCGATTTT
    GGAGATGCTG TCCAACTCAA CACGACCTAC TACATCCATC AGTTACTGGG GAACCCCGAG
    TTTGCAGCCC CCGAAATCAT CCTTGGGAAC CCCATCTCCC TGACCTCGGA TACGTGGAGT
    GTCGGAGTGC TCGCTTACGT GCTTCTCAGC GGCGTGTCCC CCTTCCTCGA CGACAGTGTG
    GAGGAGACCT GCCTGAACAT TTGCCGACTA GACTTTAGTT TCCCGGAGGA CTGCTTTAAA
    GGTGTGAGCC AGAAGGCCAA GGAGTTCGTG TGTTTCCTTC TCCAGGAGGA CCCTGCCAGG
    CGTCCCTCGG CTGCGCTGGC CCTGCAGGAG CAGTGGCTGC AGGCGGGCCA CGGCGGTGGC
    AAAGGCGCGG GCGTCCTCGA CACGTCCAGA CTGACTTCCT TCATTGAGCG GCGCAAACAC
    CAGAACGATG TTCGACCTAT CCGTAGCGTT AAAAACTTCT TACAGAGCAG GCTTTTGCCT
    AGAGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965788.1
    CDS16..9384
    Translation

    Target ORF information:

    RefSeq Version XM_014110313.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014110313.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    ATGAAGGAAT TCTTCATTTC CCTACTTTTT CTAAACCATT GTTCCCTGAA ACTGCTGCTT 
    TTAGGGCAGA GATTTAATCT GTCCTTTATT GCTCTATATT TGCCAACAGG AGCAGTGAGT
    TGTATGTGTG GTGGTGCTAA GACCACACAG CGAAGGCTTT CTTGTGCTGC CAGAGATCGA
    AAGGGAGGGT TTCGAAAAAA CGATGAAATG AAAGCTATGG ATGTTTTGCC AATTTTAAAG
    GAAAAAGTTG CATACCTTTC AGGTGGCAGA GATAAACGTG GAGGTCCCAT TTTAACATTT
    CCTGCTCGCA GCAATCATGA TAGAATACGA CAGGAGGACC TCAGGAGACT CATTTCCTAT
    CTAGCCTGTA TCCCTAGTGA AGAGGTCTGC AAGCGAGGAT TCACGGTGAT CGTGGACATG
    CGTGGGTCCA AGTGGGACTC CATTAAGCCT CTGCTGAAGA TATTGCAAGA GTCCTTTCCC
    TGCTGCATTC ATGTTGCACT GATAATCAAG CCAGACAACT TTTGGCAGAA ACAGAGGACT
    AATTTTGGCA GTTCTAAGTT TGAATTTGAG ACAAATATGG TCTCTTTAGA AGGCCTTACC
    AAAGTAGTTG ATCCTTCTCA GCTAACTCCC GAGTTTGATG GCTGCTTGGA ATACAACCAT
    GAAGAATGGA TTGAAATCAG AGTTGCTTTT GAAGACTACA TTAGCAATGC AACCCACATG
    CTCTCTCGGC TGGAGGAACT TCAGGACATC CTAGCCAAGA AAGAATTGCC TCAGGATTTA
    GAGGGGGCTC GGAATATGAT TGAGGAACAT TCCCAGCTAA AGAAGAAGGT GATTAAGGCC
    CCTATAGAAG ACTTAGATTT AGAGGGACAG AAGCTGCTCC AGAGGATTCA GAGCAGTGAC
    AGCTTTCCCA AAAAGAACTC TGGGTCAGGC AATGCGGACC TGCAGAGCCT CCTACCCAAG
    GTGTCCGCCA TGTTGGACAG GCTGCACTCA ACACGGCAGC ATCTGCATCA GATGTGGCAT
    GTACGAAAAC TGAAGCTGGA CCAGTGCTTT CAGCTAAGGC TGTTTGAACA GGACGCTGAA
    AAGATGTTTG ACTGGATCAC ACATAACAAG GGCCTGTTTC TGAACAGCTA CACTGAAATT
    GGGACCAGCC ATCCTCATGC AATGGAACTC CAGACACAGC ACAATCACTT TGCCATGAAC
    TGTATGAACG TGTATGTTAA TATAAATCGC ATAATGTCAG TGGCAAATCG CTTGGTAGAG
    TCTGGCCACT ACGCCTCTCA GCAGATTAAG CAGATTGCGA ATCAGCTGGA GCAGGAGTGG
    AAGGCATTTG CGGCAGCCCT GGATGAGCGG AGTACCTTGC TGGACATGTC CTCCATTTTC
    CACCAGAAGG CTGAAAAGTA TATGAGCAAC GTGGATTCGT GGTGTAAAGC CTGTGGTGAG
    GTAGACCTTC CCTCGGAGCT GCAGGATCTG GAGGATGCCA TTCACCACCA CCAGGGAATA
    TATGAGCACA TCACCCTTGC TTATTCCGAG GTGAGCCAGG ATGGGAAGTC ACTCCTCGAC
    AAGCTCCAGC GGCCTTTGAC TCCTGGCAGC TCCGACTCCC TCACGGCCTC TGCCAACTAC
    TCCAAGGCTG TGCACCATGT CCTTGATGTC ATCCATGAGG TGCTGCACCA CCAGCGACAG
    CTTGAGAACA TCTGGCAGCA CCGCAAGGTC CGGCTCCACC AGCGGCTGCA GCTGTGTGTG
    TTCCAGCAAG ACGTTCAGCA GGTGCTGGAC TGGATTGAGA ACCATGGAGA AGCATTTCTG
    AGCAAACATA CAGGTGTGGG GAAATCTCTT CACCGGGCCA GAGCTTTGCA GAAACGTCAT
    GAAGATTTTG AAGAAGTAGC ACAGAACACA TATACCAACG CAGATAAATT ACTAGAAGCA
    GCAGAACAGC TGGCTCAGAC CGGGGAATGT GACCCAGAAG AGATTTATCA GGCTGCGCAT
    CAGCTTGAAG ACCGGATACA AGATTTTGTT CGGCGCGTAG AGCAGCGCAA GATCCTGCTG
    GACATGTCAG TGTCGTTTCA TAGCCACGTC AAGGAGCTGT GGACATGGCT GGAGGAGCTG
    CAGAAGGAGC TGCTGGACGA TGTGTACGCC GAGTCGGTGG AGGCCGTGCA GGACCTCATC
    AAGCGCTTTG GCCAACAGCA GCAGACGACC CTGCAAGTGA CGGTCAACGT GATCAAGGAA
    GGGGAGGACC TCATACAGCA GCTGAGGGAC TCAGCCATCT CCAGCAACAA GACCCCCCAC
    AACAGCTCCA TCAGTCACAT CGAGACGGTG CTTCAGCAGC TGGACGAGGC CCAGGCCCAG
    ATGGAGGAGC TCTTCCAGGA GCGCAAAATC AAGCTGGAGC TCTTCCTGCA GCTGCGCATA
    TTCGAGAGGG ATGCCATCGA TATCATCTCA GACCTTGAGT CTTGGAACGA TGAGCTTTCT
    CAGCAAATGA ACGACTTTGA CACAGAAGAT CTCACAATAG CAGAACAGCG CCTCCAGCAC
    CATGCAGACA AAGCTTTAAC CATGAACAAC TTGACCTTTG ATGTCATCCA CCAAGGGCAG
    GACCTCTTGC AGTATGTCAA TGAAGTGCAG GCCTCTGGTG TGGAGCTGCT TTGCGATAGA
    GATGTCGACA TGGCCACTCG GGTCCAGGAC CTCCTGGAGT TCCTTCATGA AAAGCAGCAG
    GAACTGGATT TAGCAGCAGA ACAGCATCGG AAGCACTTGG AGCAGTGTGT GCAGCTTCGC
    CATCTGCAGG CTGAAGTGAA GCAGGTGCTG GGCTGGATCC GCAATGGAGA GTCAATGCTG
    AATGCTGGAC TCATCACGGC CAGCTCATTA CAGGAGGCGG AGCAGCTGCA GAGGGAGCAC
    GAGCAGTTCC AGCACGCCAT CGAGAAAACA CATCAAAGCG CCTTGCAGGT GCAGCAGAAG
    GCAGAAGCCA TGCTGCAGGC CAATCACTAT GACATGGACA TGATCAGGGA CTGCGCCGAG
    AAAGTGGCCT CTCACTGGCA GCAGCTCATG CTCAAGATGG AAGACCGCCT CAAGCTTGTG
    AATGCATCTG TGGCCTTCTA CAAAACCTCA GAGCAGGTCT GCAGTGTCCT CGAGAGCCTG
    GAGCAGGAGT ACAAGAGAGA AGAGGACTGG TGTGGAGGAG CCGACAAACT GGGGCCCAAC
    TCAGAGACGG ACCATGTCAC CCCAATGATC AGCAAGCATC TGGAGCAGAA AGAAGCATTC
    CTGAAGGCTT GCACGCTTGC TAGAAGGAAT GCAGATGTCT TCCTGAAATA CCTGCACAGG
    AATAGTGTGA ACATGCCGGG AATGGTGACA CATATCAAAG CCCCTGAACA ACAAGTGAAA
    AATATCTTGA ATGAACTTTT CCAACGGGAG AACAGGGTAC TGCATTATTG GACCATGAGA
    AAGAGACGAC TAGACCAGTG CCAGCAGTAC GTGGTCTTTG AACGAAGTGC CAAGCAGGCT
    TTGGAATGGA TCCATGACAA TGGCGAGTTC TACCTCTCCA CACACACGTC CACGGGCTCC
    AGTATACAGC ACACCCAGGA GCTACTGAAA GAGCACGAGG AGTTTCAGAT CACGGCAAAG
    CAAACCAAAG AGAGAGTGAA GCTATTGATA CAGCTGGCTG ATGGCTTTTG TGAAAAAGGG
    CATGCCCATG CAGCAGAGAT AAAAAAATGT GTCACTGCAG TGGATAAGAG GTACAGAGAT
    TTCTCTCTGC GGATGGAGAA GTACAGGACC TCTTTGGAGA AAGCCCTGGG GATTTCTTCA
    GATTCCAACA AATCGAGCAA AAGTCTTCAG CTAGACATAA TCCCTGCCAG TGTCCCTGGG
    TCAGAGGTGA AGCTTCGTGA TGCTGCTCAT GAACTTAATG AAGAGAAACG GAAGTCGGCC
    CGCAGAAAAG AGTTCATAAT GGCTGAGCTG ATTCAGACTG AAAAGGCTTA CGTGAGAGAC
    CTCCGGGAGT GTATGGATAC ATACCTGTGG GAAATGACCA GCGGAGTGGA GGAGATCCCG
    GCCGGCATCG TAAACAGAGA GCTTGTTATC TTCGGGAACA TGCAGGAAAT CTATGAGTTT
    CATAATAACA TATTCCTAAA AGAGCTGGAA AAATATGAGC AGTTGCCAGA AGATGTTGGA
    CATTGCTTTG TTACTTGGGC AGACAAGTTT CAGATGTATG TCACATATTG CAAAAATAAG
    CCTGATTCTA CTCAGCTGAT ATTAGAGCAT GCAGGATCCT ACTTTGATGA AATACAACAA
    CGGCATGGAT TAGCCAATTC CATCTCTTCC TACCTTATTA AGCCAGTTCA GCGAATAACG
    AAGTATCAAC TTCTTTTAAA AGAACTACTT ACTTGCTGTG AGGAAGGAAA GGGAGAGATA
    AAAGATGGCC TGGAGGTGAT GCTCAGTGTG CCAAAACGAG CCAATGATGC CATGCACCTC
    AGCATGCTGG AAGGGTTTGA TGAAAATATT GAGTCTCAGG GAGAACTCAT TCTACAAGAA
    TCCTTCCAAG TGTGGGACCC AAAAACCTTA ATTCGAAAGG GTCGAGAACG GCATCTCTTC
    CTTTTTGAAA TGTCCTTAGT ATTTAGTAAA GAAGTGAAAG ATTCCAGTGG CAGAAGCAAG
    TACCTTTATA AAAGCAAATT GTTTACCTCA GAGTTGGGTG TCACAGAACA CGTTGAAGGA
    GATCCTTGCA AATTTGCACT GTGGGTGGGG AGGACACCAA CCTCAGATAA TAAAATCGTC
    CTTAAGGCTT CCAGTATAGA AAACAAGCAG GACTGGATAA AGCACATCCG CGAGGTCATA
    CAGGAAAGGA CCATTCACCT GAAAGGAGCC CTGAAGGAGC CCATTCACAT TCCCAAAACG
    GCGCCGGCCA CCAGACAGAA GGGCAGGAGG GATGGAGAGG ATCTGGATAG CCAGGGCGAT
    GGGAGCAGCC AGCCTGACAC AATCTCTATC GCCTCGAGGA CGTCTCAGAA CACACTGGAT
    AGTGATAAGC TCTCTGGCGG CTGCGAGCTG ACAGTGGTGA TCCACGACTT TACCGCCTGC
    AACAGCACAG AGCTGACCAT CCGCCGTGGC CAGACCGTGG AGGTTTTGGA GCGGCCCCAC
    GACAAGCCTG ACTGGTGTCT GGTGCGGACC ACCGACCGGT CCCCTGCTGC AGAAGGCCTG
    GTTCCCTGTG GCTCTCTCTG TATCGCCCAC TCGAGAAGTA GCATGGAAAT GGAGGGTATC
    TTCAACCACA AAGACTCGCT GTCCGTCTCC AGTAACGATG CCAGCCCGCC GGCGTCCGTG
    GCCTCCCTCC AGCCCCACCT GATGGGGGCC CAGAGCTCGC CAGGCCCCAA GCGCCCAGGG
    AACACTCTGC GCAAGTGGCT GACGAGCCCG GTGCGGCGGC TGAGCAGCGG GAAGGCGGAC
    GGGCACGTGA AGAAGCTGGC GCACAAGCAC AAGAAGAGCA GGGAGGTCCG CAAGAGCACC
    GACGCGGGCT CGCAGAAGGA CTCGGACGAC AGCGCGGCCA CCCCGCAGGA CGAGACTGTC
    GAGGAGAGAG GCCGGAATGA AGGCCTGAGC AGTGGCACAC TCTCGAAGTC GTCATCGTCG
    GGCATGCAAA GCTGTGGAGA GGAAGAAGGG GAGGAGGGGG CCGACGCCGT GCCTCTCCCC
    CCGCCCATGG CCATCCAGCA GCACAGCCTC CTCCAGCCGG ATTCACAGGA CGACAAGGCG
    TCTTCTCGGC TATTAGTTCG CCCCACCAGC TCTGAAACGC CAAGCGCAGC CGAGCTCGTG
    AGTGCAATTG AAGAACTCGT GAAAAGCAAG ATGGCCTTGG AGGACCGTCC CAGCTCACTC
    CTTGTTGACC AGGGAGACAG CAGCAGCCCC TCCTTCAACC CTTCAGACAA CTCCCTCCTG
    TCCTCCTCCT CGCCCATTGA TGAGATGGAA GAAAGGAAAT CCAGCTCTTT AAAGAGACGG
    CACTATGTTT TGCAAGAACT AGTGGAGACA GAACGTGACT ATGTGCGAGA CCTCGGCTAT
    GTAGTTGAGG GCTACATGGC TCTCATGAAA GAAGATGGTG TTCCTGATGA CATGAAAGGA
    AAGGACAAAA TTGTATTTGG CAACATCCAT CAGATTTATG ACTGGCACAG AGACTTTTTT
    TTAGGAGAGT TGGAGAAGTG CCTTGAAGAT CCAGAAAAAC TAGGATCCCT TTTTGTTAAA
    CACGAAAGAA GGTTGCACAT GTACATAGTT TATTGTCAAA ATAAACCAAA GTCTGAGCAC
    ATTGTCTCAG AATACATTGA TACCTTTTTT GAGGACTTAA AGCAGCGTCT GGGCCACAGG
    TTGCAGCTCA CAGATCTGTT GATCAAACCA GTGCAGCGGA TTATGAAGTA CCAGCTGCTC
    CTGAAGGACT TCCTCAAGTA TTCCAAAAAA GCTAGCCTGG ATACATCAGA ATTAGAGAGA
    GCTGTGGAAG TCATGTGCAT AGTCCCCAAG CGGTGCAACG ACATGATGAA CGTGGGGCGG
    CTGCAAGGAT TTGATGGTAA AATTGTTGCC CAGGGCAAGC TACTCTTGCA GGACACATTC
    TTGGTCACAG ACCAGGACTC GGGACTTCTG CCTCGCTGTA AAGAGAGGCG AGTTTTCCTC
    TTTGAGCAAA TCGTCATTTT CAGTGAACCA CTTGATAAAA AAAAGGGCTT CTCGATGCCA
    GGATTCCTGT TTAAGAACAG TATCAAGGTG AGTTGCCTTT GCCTGGAGGA AAATGTGGAA
    AATGATCCAT GTAAATTTGC TTTGACGTCG AGGACGGGTG GTGTGGTGGA GACCTTCATT
    TTGCATTCAT CCAGTCCAAG TGTCCGGCAA ACATGGATCC ATGAAATCAA CCAAATTTTA
    GAAAACCAGC GCAATTTTTT AAATGCCTTG ACATCACCAA TCGAGTATCA GAGGAACCAC
    AGTGGGGGCG GCGGCGGGGG CAGTGGCGGG GGCGGCGGCA GCGGAGGCAG CGGCGGCGGG
    GCTGGCAGCG GGGCCCCCGG CGGCGGAGGC AGCAACCCCA GCGGCGGCCC TGGCAGCTGC
    AGCGGCCTCC CCAGCTCGAG CAGAAGCCGA CCCTCCCGGA TCCCCCAGCC TGTCAGACAC
    CACTCCCCCG TGCTGGTCTC CTCTGCAGCC TCGGGCCAGG CAGAGGCAGA CAAGATGTCA
    GGTACGTCCA CTCCCGGCCC TGCACTGGCC CCTCCCGGCA GCAGCCCCGC CCCGGAGCCC
    GGCCCCAGCC AGCCTGGCAG GCGCGCCCCC GAGCCCGGAG GCCCCGAGCG AGAAGCCGAG
    CTCATCCCCA GGATGAAGGT GATTGAGAGC CCCCGGAGGT GCTCGGGGGG CGCGGCGGGC
    GCCGGCCGCG ATGCGGACAC CAAGGAGGCG CGGGCCCCCC CGGAGGACAG CCGCTCGCGG
    AGCAGCCCGG GCTCGCTGCC CCTGGCAAAG CCCAGGCCTG GGGCCACCTC GCCTCTGAAC
    TCTCCCGTGG CCGCCGCATT ACCTCCTCCA ATCGGCAAGG AGCCCTGTCC CCCCAGCAGC
    CCCCTGCAGA AGGGGGGCTC CTTCTGGAGC TCCATCCCCG CCTCCCCCGC CAGCCGGCCA
    GGCTCCTTCA CCTTCCCGGG GGACAGTGAC TCCCTCCAGC GGCAGGCCCA GCGCCACGCG
    GCCCCCAGCA AGGACACAGA CCGCATGAGC ACCTGCTCGT CGGCCAGCGA GCAGTCGGTA
    CAGTCCACCC AGAGCAACGG GAGTGAAAGT AGCAGCAGTA GCAACATCTC CACCATGTTG
    GTGACACACG ATTACACGGC AGTGAAGGAG GATGAGATAA ACGTCTATCA AGGAGAGGTC
    GTTCAAATTC TGGCCAGCAA TCAACAGAAC ATGTTTCTGG TGTTCCGAGC CGCCACTGAC
    CAGTGCCCCG CAGCCGAGGG CTGGATTCCA GGCTTCGTCC TGGGGCATAC CAGTGCAGTC
    ATCATGGAGA ACCCCGATGG GACTCTCAAG AAGTCAACAT CTTGGCACAC AGCACTCCGT
    TTAAGGAAAA AATCTGAGAA AAAAGATAAA GACGGCAAGC GGGAAGGCAA GTTAGAGAAT
    GGTTATCGGA AGTCACGGGA GGGGCTCAGC AGCAAGGTAT CTGTGAAGCT TCTCAATCCC
    AACTACATAT ATGACGTTCC CCCAGAATTC GTCATTCCCT TGAGTGAGGT CACGTGTGAG
    ACAGGGGAGA CCGTTGTTTT TAGATGTCGG GTTTGTGGCC GCCCCAAGGC CTCAATCACC
    TGGAAGGGCC CTGAGCACAA CACCCTGAAC AACGATGGTC ACTACAGCAT CTCCTACAGT
    GACTTGGGAG AGGCCGCTTT GAAGATCGTC GGCGTGACCA CAGAAGATGA TGGCATCTAC
    ACATGCATCG CTGTGAACGA CATGGGTTCA GCCTCGTCTT CGGCCAGTCT GAGGGTGCTG
    GGTCCAGGGA GTGATGGGAT CATGGTGACC TGGAAAGACA ACTTTGACTC CTTCTACAGC
    GAAGTGGCTG AGCTTGGCAG GGGCAGATTC TCTGTCGTTA AGAAATGTGA TCAGAAAGGA
    ACCAAACGAG CAGTGGCCAC TAAATTTGTG AACAAGAAGT TGATGAAGCG TGACCAGGTC
    ACCCATGAAC TTGGAATCCT GCAGAACCTC CAGCACCCGC TGCTTATTGG TCTCCTTGAT
    ACCTTCGAGA CCCCCACCAG CTACGTCCTG GTGTTAGAAA TGGCTGACCA GGGGCGTCTC
    CTGGACTGTG TGGTGCGATG GGGAGACCTC ACTGAAGGGA AGATCAGGGC GTATCTGGGG
    GAGGTTCTGG AAGCCGTCCA ATACCTTCAC AACTGCAGGA TAGCACACCT GGACCTAAAG
    CCTGAAAACA TTCTGGTGGA TCAGAGTCTG GCCAAGCCCA CGATCAAACT GGCCGATTTT
    GGAGATGCTG TCCAACTCAA CACGACCTAC TACATCCATC AGTTACTGGG GAACCCCGAG
    TTTGCAGCCC CCGAAATCAT CCTTGGGAAC CCCATCTCCC TGACCTCGGA TACGTGGAGT
    GTCGGAGTGC TCGCTTACGT GCTTCTCAGC GGCGTGTCCC CCTTCCTCGA CGACAGTGTG
    GAGGAGACCT GCCTGAACAT TTGCCGACTA GACTTTAGTT TCCCGGAGGA CTGCTTTAAA
    GGTGTGAGCC AGAAGGCCAA GGAGTTCGTG TGTTTCCTTC TCCAGGAGGA CCCTGCCAGG
    CGTCCCTCGG CTGCGCTGGC CCTGCAGGAG CAGTGGCTGC AGGCGGGCCA CGGCGGTGGC
    AAAGGCGCGG GCGTCCTCGA CACGTCCAGA CTGACTTCCT TCATTGAGCG GCGCAAACAC
    CAGAACGATG TTCGACCTAT CCGTAGCGTT AAAAACTTCT TACAGAGCAG GCTTTTGCCT
    AGAGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44776
    Clone ID Related Accession (Same CDS sequence) XM_014110314.1
    Accession Version XM_014110314.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product triple functional domain protein isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726119.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGAGGCCTT TGGAAATGGA TGAAGAGCAC AGTTTGTCCT GGTTTCGAAA AAACGATGAA 
    ATGAAAGCTA TGGATGTTTT GCCAATTTTA AAGGAAAAAG TTGCATACCT TTCAGGTGGC
    AGAGATAAAC GTGGAGGTCC CATTTTAACA TTTCCTGCTC GCAGCAATCA TGATAGAATA
    CGACAGGAGG ACCTCAGGAG ACTCATTTCC TATCTAGCCT GTATCCCTAG TGAAGAGGTC
    TGCAAGCGAG GATTCACGGT GATCGTGGAC ATGCGTGGGT CCAAGTGGGA CTCCATTAAG
    CCTCTGCTGA AGATATTGCA AGAGTCCTTT CCCTGCTGCA TTCATGTTGC ACTGATAATC
    AAGCCAGACA ACTTTTGGCA GAAACAGAGG ACTAATTTTG GCAGTTCTAA GTTTGAATTT
    GAGACAAATA TGGTCTCTTT AGAAGGCCTT ACCAAAGTAG TTGATCCTTC TCAGCTAACT
    CCCGAGTTTG ATGGCTGCTT GGAATACAAC CATGAAGAAT GGATTGAAAT CAGAGTTGCT
    TTTGAAGACT ACATTAGCAA TGCAACCCAC ATGCTCTCTC GGCTGGAGGA ACTTCAGGAC
    ATCCTAGCCA AGAAAGAATT GCCTCAGGAT TTAGAGGGGG CTCGGAATAT GATTGAGGAA
    CATTCCCAGC TAAAGAAGAA GGTGATTAAG GCCCCTATAG AAGACTTAGA TTTAGAGGGA
    CAGAAGCTGC TCCAGAGGAT TCAGAGCAGT GACAGCTTTC CCAAAAAGAA CTCTGGGTCA
    GGCAATGCGG ACCTGCAGAG CCTCCTACCC AAGGTGTCCG CCATGTTGGA CAGGCTGCAC
    TCAACACGGC AGCATCTGCA TCAGATGTGG CATGTACGAA AACTGAAGCT GGACCAGTGC
    TTTCAGCTAA GGCTGTTTGA ACAGGACGCT GAAAAGATGT TTGACTGGAT CACACATAAC
    AAGGGCCTGT TTCTGAACAG CTACACTGAA ATTGGGACCA GCCATCCTCA TGCAATGGAA
    CTCCAGACAC AGCACAATCA CTTTGCCATG AACTGTATGA ACGTGTATGT TAATATAAAT
    CGCATAATGT CAGTGGCAAA TCGCTTGGTA GAGTCTGGCC ACTACGCCTC TCAGCAGATT
    AAGCAGATTG CGAATCAGCT GGAGCAGGAG TGGAAGGCAT TTGCGGCAGC CCTGGATGAG
    CGGAGTACCT TGCTGGACAT GTCCTCCATT TTCCACCAGA AGGCTGAAAA GTATATGAGC
    AACGTGGATT CGTGGTGTAA AGCCTGTGGT GAGGTAGACC TTCCCTCGGA GCTGCAGGAT
    CTGGAGGATG CCATTCACCA CCACCAGGGA ATATATGAGC ACATCACCCT TGCTTATTCC
    GAGGTGAGCC AGGATGGGAA GTCACTCCTC GACAAGCTCC AGCGGCCTTT GACTCCTGGC
    AGCTCCGACT CCCTCACGGC CTCTGCCAAC TACTCCAAGG CTGTGCACCA TGTCCTTGAT
    GTCATCCATG AGGTGCTGCA CCACCAGCGA CAGCTTGAGA ACATCTGGCA GCACCGCAAG
    GTCCGGCTCC ACCAGCGGCT GCAGCTGTGT GTGTTCCAGC AAGACGTTCA GCAGGTGCTG
    GACTGGATTG AGAACCATGG AGAAGCATTT CTGAGCAAAC ATACAGGTGT GGGGAAATCT
    CTTCACCGGG CCAGAGCTTT GCAGAAACGT CATGAAGATT TTGAAGAAGT AGCACAGAAC
    ACATATACCA ACGCAGATAA ATTACTAGAA GCAGCAGAAC AGCTGGCTCA GACCGGGGAA
    TGTGACCCAG AAGAGATTTA TCAGGCTGCG CATCAGCTTG AAGACCGGAT ACAAGATTTT
    GTTCGGCGCG TAGAGCAGCG CAAGATCCTG CTGGACATGT CAGTGTCGTT TCATAGCCAC
    GTCAAGGAGC TGTGGACATG GCTGGAGGAG CTGCAGAAGG AGCTGCTGGA CGATGTGTAC
    GCCGAGTCGG TGGAGGCCGT GCAGGACCTC ATCAAGCGCT TTGGCCAACA GCAGCAGACG
    ACCCTGCAAG TGACGGTCAA CGTGATCAAG GAAGGGGAGG ACCTCATACA GCAGCTGAGG
    GACTCAGCCA TCTCCAGCAA CAAGACCCCC CACAACAGCT CCATCAGTCA CATCGAGACG
    GTGCTTCAGC AGCTGGACGA GGCCCAGGCC CAGATGGAGG AGCTCTTCCA GGAGCGCAAA
    ATCAAGCTGG AGCTCTTCCT GCAGCTGCGC ATATTCGAGA GGGATGCCAT CGATATCATC
    TCAGACCTTG AGTCTTGGAA CGATGAGCTT TCTCAGCAAA TGAACGACTT TGACACAGAA
    GATCTCACAA TAGCAGAACA GCGCCTCCAG CACCATGCAG ACAAAGCTTT AACCATGAAC
    AACTTGACCT TTGATGTCAT CCACCAAGGG CAGGACCTCT TGCAGTATGT CAATGAAGTG
    CAGGCCTCTG GTGTGGAGCT GCTTTGCGAT AGAGATGTCG ACATGGCCAC TCGGGTCCAG
    GACCTCCTGG AGTTCCTTCA TGAAAAGCAG CAGGAACTGG ATTTAGCAGC AGAACAGCAT
    CGGAAGCACT TGGAGCAGTG TGTGCAGCTT CGCCATCTGC AGGCTGAAGT GAAGCAGGTG
    CTGGGCTGGA TCCGCAATGG AGAGTCAATG CTGAATGCTG GACTCATCAC GGCCAGCTCA
    TTACAGGAGG CGGAGCAGCT GCAGAGGGAG CACGAGCAGT TCCAGCACGC CATCGAGAAA
    ACACATCAAA GCGCCTTGCA GGTGCAGCAG AAGGCAGAAG CCATGCTGCA GGCCAATCAC
    TATGACATGG ACATGATCAG GGACTGCGCC GAGAAAGTGG CCTCTCACTG GCAGCAGCTC
    ATGCTCAAGA TGGAAGACCG CCTCAAGCTT GTGAATGCAT CTGTGGCCTT CTACAAAACC
    TCAGAGCAGG TCTGCAGTGT CCTCGAGAGC CTGGAGCAGG AGTACAAGAG AGAAGAGGAC
    TGGTGTGGAG GAGCCGACAA ACTGGGGCCC AACTCAGAGA CGGACCATGT CACCCCAATG
    ATCAGCAAGC ATCTGGAGCA GAAAGAAGCA TTCCTGAAGG CTTGCACGCT TGCTAGAAGG
    AATGCAGATG TCTTCCTGAA ATACCTGCAC AGGAATAGTG TGAACATGCC GGGAATGGTG
    ACACATATCA AAGCCCCTGA ACAACAAGTG AAAAATATCT TGAATGAACT TTTCCAACGG
    GAGAACAGGG TACTGCATTA TTGGACCATG AGAAAGAGAC GACTAGACCA GTGCCAGCAG
    TACGTGGTCT TTGAACGAAG TGCCAAGCAG GCTTTGGAAT GGATCCATGA CAATGGCGAG
    TTCTACCTCT CCACACACAC GTCCACGGGC TCCAGTATAC AGCACACCCA GGAGCTACTG
    AAAGAGCACG AGGAGTTTCA GATCACGGCA AAGCAAACCA AAGAGAGAGT GAAGCTATTG
    ATACAGCTGG CTGATGGCTT TTGTGAAAAA GGGCATGCCC ATGCAGCAGA GATAAAAAAA
    TGTGTCACTG CAGTGGATAA GAGGTACAGA GATTTCTCTC TGCGGATGGA GAAGTACAGG
    ACCTCTTTGG AGAAAGCCCT GGGGATTTCT TCAGATTCCA ACAAATCGAG CAAAAGTCTT
    CAGCTAGACA TAATCCCTGC CAGTGTCCCT GGGTCAGAGG TGAAGCTTCG TGATGCTGCT
    CATGAACTTA ATGAAGAGAA ACGGAAGTCG GCCCGCAGAA AAGAGTTCAT AATGGCTGAG
    CTGATTCAGA CTGAAAAGGC TTACGTGAGA GACCTCCGGG AGTGTATGGA TACATACCTG
    TGGGAAATGA CCAGCGGAGT GGAGGAGATC CCGGCCGGCA TCGTAAACAG AGAGCTTGTT
    ATCTTCGGGA ACATGCAGGA AATCTATGAG TTTCATAATA ACATATTCCT AAAAGAGCTG
    GAAAAATATG AGCAGTTGCC AGAAGATGTT GGACATTGCT TTGTTACTTG GGCAGACAAG
    TTTCAGATGT ATGTCACATA TTGCAAAAAT AAGCCTGATT CTACTCAGCT GATATTAGAG
    CATGCAGGAT CCTACTTTGA TGAAATACAA CAACGGCATG GATTAGCCAA TTCCATCTCT
    TCCTACCTTA TTAAGCCAGT TCAGCGAATA ACGAAGTATC AACTTCTTTT AAAAGAACTA
    CTTACTTGCT GTGAGGAAGG AAAGGGAGAG ATAAAAGATG GCCTGGAGGT GATGCTCAGT
    GTGCCAAAAC GAGCCAATGA TGCCATGCAC CTCAGCATGC TGGAAGGGTT TGATGAAAAT
    ATTGAGTCTC AGGGAGAACT CATTCTACAA GAATCCTTCC AAGTGTGGGA CCCAAAAACC
    TTAATTCGAA AGGGTCGAGA ACGGCATCTC TTCCTTTTTG AAATGTCCTT AGTATTTAGT
    AAAGAAGTGA AAGATTCCAG TGGCAGAAGC AAGTACCTTT ATAAAAGCAA ATTGTTTACC
    TCAGAGTTGG GTGTCACAGA ACACGTTGAA GGAGATCCTT GCAAATTTGC ACTGTGGGTG
    GGGAGGACAC CAACCTCAGA TAATAAAATC GTCCTTAAGG CTTCCAGTAT AGAAAACAAG
    CAGGACTGGA TAAAGCACAT CCGCGAGGTC ATACAGGAAA GGACCATTCA CCTGAAAGGA
    GCCCTGAAGG AGCCCATTCA CATTCCCAAA ACGGCGCCGG CCACCAGACA GAAGGGCAGG
    AGGGATGGAG AGGATCTGGA TAGCCAGGGC GATGGGAGCA GCCAGCCTGA CACAATCTCT
    ATCGCCTCGA GGACGTCTCA GAACACACTG GATAGTGATA AGCTCTCTGG CGGCTGCGAG
    CTGACAGTGG TGATCCACGA CTTTACCGCC TGCAACAGCA CAGAGCTGAC CATCCGCCGT
    GGCCAGACCG TGGAGGTTTT GGAGCGGCCC CACGACAAGC CTGACTGGTG TCTGGTGCGG
    ACCACCGACC GGTCCCCTGC TGCAGAAGGC CTGGTTCCCT GTGGCTCTCT CTGTATCGCC
    CACTCGAGAA GTAGCATGGA AATGGAGGGT ATCTTCAACC ACAAAGACTC GCTGTCCGTC
    TCCAGTAACG ATGCCAGCCC GCCGGCGTCC GTGGCCTCCC TCCAGCCCCA CCTGATGGGG
    GCCCAGAGCT CGCCAGGCCC CAAGCGCCCA GGGAACACTC TGCGCAAGTG GCTGACGAGC
    CCGGTGCGGC GGCTGAGCAG CGGGAAGGCG GACGGGCACG TGAAGAAGCT GGCGCACAAG
    CACAAGAAGA GCAGGGAGGT CCGCAAGAGC ACCGACGCGG GCTCGCAGAA GGACTCGGAC
    GACAGCGCGG CCACCCCGCA GGACGAGACT GTCGAGGAGA GAGGCCGGAA TGAAGGCCTG
    AGCAGTGGCA CACTCTCGAA GTCGTCATCG TCGGGCATGC AAAGCTGTGG AGAGGAAGAA
    GGGGAGGAGG GGGCCGACGC CGTGCCTCTC CCCCCGCCCA TGGCCATCCA GCAGCACAGC
    CTCCTCCAGC CGGATTCACA GGACGACAAG GCGTCTTCTC GGCTATTAGT TCGCCCCACC
    AGCTCTGAAA CGCCAAGCGC AGCCGAGCTC GTGAGTGCAA TTGAAGAACT CGTGAAAAGC
    AAGATGGCCT TGGAGGACCG TCCCAGCTCA CTCCTTGTTG ACCAGGGAGA CAGCAGCAGC
    CCCTCCTTCA ACCCTTCAGA CAACTCCCTC CTGTCCTCCT CCTCGCCCAT TGATGAGATG
    GAAGAAAGGA AATCCAGCTC TTTAAAGAGA CGGCACTATG TTTTGCAAGA ACTAGTGGAG
    ACAGAACGTG ACTATGTGCG AGACCTCGGC TATGTAGTTG AGGGCTACAT GGCTCTCATG
    AAAGAAGATG GTGTTCCTGA TGACATGAAA GGAAAGGACA AAATTGTATT TGGCAACATC
    CATCAGATTT ATGACTGGCA CAGAGACTTT TTTTTAGGAG AGTTGGAGAA GTGCCTTGAA
    GATCCAGAAA AACTAGGATC CCTTTTTGTT AAACACGAAA GAAGGTTGCA CATGTACATA
    GTTTATTGTC AAAATAAACC AAAGTCTGAG CACATTGTCT CAGAATACAT TGATACCTTT
    TTTGAGGACT TAAAGCAGCG TCTGGGCCAC AGGTTGCAGC TCACAGATCT GTTGATCAAA
    CCAGTGCAGC GGATTATGAA GTACCAGCTG CTCCTGAAGG ACTTCCTCAA GTATTCCAAA
    AAAGCTAGCC TGGATACATC AGAATTAGAG AGAGCTGTGG AAGTCATGTG CATAGTCCCC
    AAGCGGTGCA ACGACATGAT GAACGTGGGG CGGCTGCAAG GATTTGATGG TAAAATTGTT
    GCCCAGGGCA AGCTACTCTT GCAGGACACA TTCTTGGTCA CAGACCAGGA CTCGGGACTT
    CTGCCTCGCT GTAAAGAGAG GCGAGTTTTC CTCTTTGAGC AAATCGTCAT TTTCAGTGAA
    CCACTTGATA AAAAAAAGGG CTTCTCGATG CCAGGATTCC TGTTTAAGAA CAGTATCAAG
    GTGAGTTGCC TTTGCCTGGA GGAAAATGTG GAAAATGATC CATGTAAATT TGCTTTGACG
    TCGAGGACGG GTGGTGTGGT GGAGACCTTC ATTTTGCATT CATCCAGTCC AAGTGTCCGG
    CAAACATGGA TCCATGAAAT CAACCAAATT TTAGAAAACC AGCGCAATTT TTTAAATGCC
    TTGACATCAC CAATCGAGTA TCAGAGGAAC CACAGTGGGG GCGGCGGCGG GGGCAGTGGC
    GGGGGCGGCG GCAGCGGAGG CAGCGGCGGC GGGGCTGGCA GCGGGGCCCC CGGCGGCGGA
    GGCAGCAACC CCAGCGGCGG CCCTGGCAGC TGCAGCGGCC TCCCCAGCTC GAGCAGAAGC
    CGACCCTCCC GGATCCCCCA GCCTGTCAGA CACCACTCCC CCGTGCTGGT CTCCTCTGCA
    GCCTCGGGCC AGGCAGAGGC AGACAAGATG TCAGGTACGT CCACTCCCGG CCCTGCACTG
    GCCCCTCCCG GCAGCAGCCC CGCCCCGGAG CCCGGCCCCA GCCAGCCTGG CAGGCGCGCC
    CCCGAGCCCG GAGGCCCCGA GCGAGAAGCC GAGCTCATCC CCAGGATGAA GGTGATTGAG
    AGCCCCCGGA GGTGCTCGGG GGGCGCGGCG GGCGCCGGCC GCGATGCGGA CACCAAGGAG
    GCGCGGGCCC CCCCGGAGGA CAGCCGCTCG CGGAGCAGCC CGGGCTCGCT GCCCCTGGCA
    AAGCCCAGGC CTGGGGCCAC CTCGCCTCTG AACTCTCCCG TGGCCGCCGC ATTACCTCCT
    CCAATCGGCA AGGAGCCCTG TCCCCCCAGC AGCCCCCTGC AGAAGGGGGG CTCCTTCTGG
    AGCTCCATCC CCGCCTCCCC CGCCAGCCGG CCAGGCTCCT TCACCTTCCC GGGGGACAGT
    GACTCCCTCC AGCGGCAGGC CCAGCGCCAC GCGGCCCCCA GCAAGGACAC AGACCGCATG
    AGCACCTGCT CGTCGGCCAG CGAGCAGTCG GTACAGTCCA CCCAGAGCAA CGGGAGTGAA
    AGTAGCAGCA GTAGCAACAT CTCCACCATG TTGGTGACAC ACGATTACAC GGCAGTGAAG
    GAGGATGAGA TAAACGTCTA TCAAGGAGAG GTCGTTCAAA TTCTGGCCAG CAATCAACAG
    AACATGTTTC TGGTGTTCCG AGCCGCCACT GACCAGTGCC CCGCAGCCGA GGGCTGGATT
    CCAGGCTTCG TCCTGGGGCA TACCAGTGCA GTCATCATGG AGAACCCCGA TGGGACTCTC
    AAGAAGTCAA CATCTTGGCA CACAGCACTC CGTTTAAGGA AAAAATCTGA GAAAAAAGAT
    AAAGACGGCA AGCGGGAAGG CAAGTTAGAG AATGGTTATC GGAAGTCACG GGAGGGGCTC
    AGCAGCAAGG TATCTGTGAA GCTTCTCAAT CCCAACTACA TATATGACGT TCCCCCAGAA
    TTCGTCATTC CCTTGAGTGA GGTCACGTGT GAGACAGGGG AGACCGTTGT TTTTAGATGT
    CGGGTTTGTG GCCGCCCCAA GGCCTCAATC ACCTGGAAGG GCCCTGAGCA CAACACCCTG
    AACAACGATG GTCACTACAG CATCTCCTAC AGTGACTTGG GAGAGGCCGC TTTGAAGATC
    GTCGGCGTGA CCACAGAAGA TGATGGCATC TACACATGCA TCGCTGTGAA CGACATGGGT
    TCAGCCTCGT CTTCGGCCAG TCTGAGGGTG CTGGGTCCAG GGAGTGATGG GATCATGGTG
    ACCTGGAAAG ACAACTTTGA CTCCTTCTAC AGCGAAGTGG CTGAGCTTGG CAGGGGCAGA
    TTCTCTGTCG TTAAGAAATG TGATCAGAAA GGAACCAAAC GAGCAGTGGC CACTAAATTT
    GTGAACAAGA AGTTGATGAA GCGTGACCAG GTCACCCATG AACTTGGAAT CCTGCAGAAC
    CTCCAGCACC CGCTGCTTAT TGGTCTCCTT GATACCTTCG AGACCCCCAC CAGCTACGTC
    CTGGTGTTAG AAATGGCTGA CCAGGGGCGT CTCCTGGACT GTGTGGTGCG ATGGGGAGAC
    CTCACTGAAG GGAAGATCAG GGCGTATCTG GGGGAGGTTC TGGAAGCCGT CCAATACCTT
    CACAACTGCA GGATAGCACA CCTGGACCTA AAGCCTGAAA ACATTCTGGT GGATCAGAGT
    CTGGCCAAGC CCACGATCAA ACTGGCCGAT TTTGGAGATG CTGTCCAACT CAACACGACC
    TACTACATCC ATCAGTTACT GGGGAACCCC GAGTTTGCAG CCCCCGAAAT CATCCTTGGG
    AACCCCATCT CCCTGACCTC GGATACGTGG AGTGTCGGAG TGCTCGCTTA CGTGCTTCTC
    AGCGGCGTGT CCCCCTTCCT CGACGACAGT GTGGAGGAGA CCTGCCTGAA CATTTGCCGA
    CTAGACTTTA GTTTCCCGGA GGACTGCTTT AAAGGTGTGA GCCAGAAGGC CAAGGAGTTC
    GTGTGTTTCC TTCTCCAGGA GGACCCTGCC AGGCGTCCCT CGGCTGCGCT GGCCCTGCAG
    GAGCAGTGGC TGCAGGCGGG CCACGGCGGT GGCAAAGGCG CGGGCGTCCT CGACACGTCC
    AGACTGACTT CCTTCATTGA GCGGCGCAAA CACCAGAACG ATGTTCGACC TATCCGTAGC
    GTTAAAAACT TCTTACAGAG CAGGCTTTTG CCTAGAGTTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965789.1
    CDS16..9237
    Translation

    Target ORF information:

    RefSeq Version XM_014110314.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014110314.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGAGGCCTT TGGAAATGGA TGAAGAGCAC AGTTTGTCCT GGTTTCGAAA AAACGATGAA 
    ATGAAAGCTA TGGATGTTTT GCCAATTTTA AAGGAAAAAG TTGCATACCT TTCAGGTGGC
    AGAGATAAAC GTGGAGGTCC CATTTTAACA TTTCCTGCTC GCAGCAATCA TGATAGAATA
    CGACAGGAGG ACCTCAGGAG ACTCATTTCC TATCTAGCCT GTATCCCTAG TGAAGAGGTC
    TGCAAGCGAG GATTCACGGT GATCGTGGAC ATGCGTGGGT CCAAGTGGGA CTCCATTAAG
    CCTCTGCTGA AGATATTGCA AGAGTCCTTT CCCTGCTGCA TTCATGTTGC ACTGATAATC
    AAGCCAGACA ACTTTTGGCA GAAACAGAGG ACTAATTTTG GCAGTTCTAA GTTTGAATTT
    GAGACAAATA TGGTCTCTTT AGAAGGCCTT ACCAAAGTAG TTGATCCTTC TCAGCTAACT
    CCCGAGTTTG ATGGCTGCTT GGAATACAAC CATGAAGAAT GGATTGAAAT CAGAGTTGCT
    TTTGAAGACT ACATTAGCAA TGCAACCCAC ATGCTCTCTC GGCTGGAGGA ACTTCAGGAC
    ATCCTAGCCA AGAAAGAATT GCCTCAGGAT TTAGAGGGGG CTCGGAATAT GATTGAGGAA
    CATTCCCAGC TAAAGAAGAA GGTGATTAAG GCCCCTATAG AAGACTTAGA TTTAGAGGGA
    CAGAAGCTGC TCCAGAGGAT TCAGAGCAGT GACAGCTTTC CCAAAAAGAA CTCTGGGTCA
    GGCAATGCGG ACCTGCAGAG CCTCCTACCC AAGGTGTCCG CCATGTTGGA CAGGCTGCAC
    TCAACACGGC AGCATCTGCA TCAGATGTGG CATGTACGAA AACTGAAGCT GGACCAGTGC
    TTTCAGCTAA GGCTGTTTGA ACAGGACGCT GAAAAGATGT TTGACTGGAT CACACATAAC
    AAGGGCCTGT TTCTGAACAG CTACACTGAA ATTGGGACCA GCCATCCTCA TGCAATGGAA
    CTCCAGACAC AGCACAATCA CTTTGCCATG AACTGTATGA ACGTGTATGT TAATATAAAT
    CGCATAATGT CAGTGGCAAA TCGCTTGGTA GAGTCTGGCC ACTACGCCTC TCAGCAGATT
    AAGCAGATTG CGAATCAGCT GGAGCAGGAG TGGAAGGCAT TTGCGGCAGC CCTGGATGAG
    CGGAGTACCT TGCTGGACAT GTCCTCCATT TTCCACCAGA AGGCTGAAAA GTATATGAGC
    AACGTGGATT CGTGGTGTAA AGCCTGTGGT GAGGTAGACC TTCCCTCGGA GCTGCAGGAT
    CTGGAGGATG CCATTCACCA CCACCAGGGA ATATATGAGC ACATCACCCT TGCTTATTCC
    GAGGTGAGCC AGGATGGGAA GTCACTCCTC GACAAGCTCC AGCGGCCTTT GACTCCTGGC
    AGCTCCGACT CCCTCACGGC CTCTGCCAAC TACTCCAAGG CTGTGCACCA TGTCCTTGAT
    GTCATCCATG AGGTGCTGCA CCACCAGCGA CAGCTTGAGA ACATCTGGCA GCACCGCAAG
    GTCCGGCTCC ACCAGCGGCT GCAGCTGTGT GTGTTCCAGC AAGACGTTCA GCAGGTGCTG
    GACTGGATTG AGAACCATGG AGAAGCATTT CTGAGCAAAC ATACAGGTGT GGGGAAATCT
    CTTCACCGGG CCAGAGCTTT GCAGAAACGT CATGAAGATT TTGAAGAAGT AGCACAGAAC
    ACATATACCA ACGCAGATAA ATTACTAGAA GCAGCAGAAC AGCTGGCTCA GACCGGGGAA
    TGTGACCCAG AAGAGATTTA TCAGGCTGCG CATCAGCTTG AAGACCGGAT ACAAGATTTT
    GTTCGGCGCG TAGAGCAGCG CAAGATCCTG CTGGACATGT CAGTGTCGTT TCATAGCCAC
    GTCAAGGAGC TGTGGACATG GCTGGAGGAG CTGCAGAAGG AGCTGCTGGA CGATGTGTAC
    GCCGAGTCGG TGGAGGCCGT GCAGGACCTC ATCAAGCGCT TTGGCCAACA GCAGCAGACG
    ACCCTGCAAG TGACGGTCAA CGTGATCAAG GAAGGGGAGG ACCTCATACA GCAGCTGAGG
    GACTCAGCCA TCTCCAGCAA CAAGACCCCC CACAACAGCT CCATCAGTCA CATCGAGACG
    GTGCTTCAGC AGCTGGACGA GGCCCAGGCC CAGATGGAGG AGCTCTTCCA GGAGCGCAAA
    ATCAAGCTGG AGCTCTTCCT GCAGCTGCGC ATATTCGAGA GGGATGCCAT CGATATCATC
    TCAGACCTTG AGTCTTGGAA CGATGAGCTT TCTCAGCAAA TGAACGACTT TGACACAGAA
    GATCTCACAA TAGCAGAACA GCGCCTCCAG CACCATGCAG ACAAAGCTTT AACCATGAAC
    AACTTGACCT TTGATGTCAT CCACCAAGGG CAGGACCTCT TGCAGTATGT CAATGAAGTG
    CAGGCCTCTG GTGTGGAGCT GCTTTGCGAT AGAGATGTCG ACATGGCCAC TCGGGTCCAG
    GACCTCCTGG AGTTCCTTCA TGAAAAGCAG CAGGAACTGG ATTTAGCAGC AGAACAGCAT
    CGGAAGCACT TGGAGCAGTG TGTGCAGCTT CGCCATCTGC AGGCTGAAGT GAAGCAGGTG
    CTGGGCTGGA TCCGCAATGG AGAGTCAATG CTGAATGCTG GACTCATCAC GGCCAGCTCA
    TTACAGGAGG CGGAGCAGCT GCAGAGGGAG CACGAGCAGT TCCAGCACGC CATCGAGAAA
    ACACATCAAA GCGCCTTGCA GGTGCAGCAG AAGGCAGAAG CCATGCTGCA GGCCAATCAC
    TATGACATGG ACATGATCAG GGACTGCGCC GAGAAAGTGG CCTCTCACTG GCAGCAGCTC
    ATGCTCAAGA TGGAAGACCG CCTCAAGCTT GTGAATGCAT CTGTGGCCTT CTACAAAACC
    TCAGAGCAGG TCTGCAGTGT CCTCGAGAGC CTGGAGCAGG AGTACAAGAG AGAAGAGGAC
    TGGTGTGGAG GAGCCGACAA ACTGGGGCCC AACTCAGAGA CGGACCATGT CACCCCAATG
    ATCAGCAAGC ATCTGGAGCA GAAAGAAGCA TTCCTGAAGG CTTGCACGCT TGCTAGAAGG
    AATGCAGATG TCTTCCTGAA ATACCTGCAC AGGAATAGTG TGAACATGCC GGGAATGGTG
    ACACATATCA AAGCCCCTGA ACAACAAGTG AAAAATATCT TGAATGAACT TTTCCAACGG
    GAGAACAGGG TACTGCATTA TTGGACCATG AGAAAGAGAC GACTAGACCA GTGCCAGCAG
    TACGTGGTCT TTGAACGAAG TGCCAAGCAG GCTTTGGAAT GGATCCATGA CAATGGCGAG
    TTCTACCTCT CCACACACAC GTCCACGGGC TCCAGTATAC AGCACACCCA GGAGCTACTG
    AAAGAGCACG AGGAGTTTCA GATCACGGCA AAGCAAACCA AAGAGAGAGT GAAGCTATTG
    ATACAGCTGG CTGATGGCTT TTGTGAAAAA GGGCATGCCC ATGCAGCAGA GATAAAAAAA
    TGTGTCACTG CAGTGGATAA GAGGTACAGA GATTTCTCTC TGCGGATGGA GAAGTACAGG
    ACCTCTTTGG AGAAAGCCCT GGGGATTTCT TCAGATTCCA ACAAATCGAG CAAAAGTCTT
    CAGCTAGACA TAATCCCTGC CAGTGTCCCT GGGTCAGAGG TGAAGCTTCG TGATGCTGCT
    CATGAACTTA ATGAAGAGAA ACGGAAGTCG GCCCGCAGAA AAGAGTTCAT AATGGCTGAG
    CTGATTCAGA CTGAAAAGGC TTACGTGAGA GACCTCCGGG AGTGTATGGA TACATACCTG
    TGGGAAATGA CCAGCGGAGT GGAGGAGATC CCGGCCGGCA TCGTAAACAG AGAGCTTGTT
    ATCTTCGGGA ACATGCAGGA AATCTATGAG TTTCATAATA ACATATTCCT AAAAGAGCTG
    GAAAAATATG AGCAGTTGCC AGAAGATGTT GGACATTGCT TTGTTACTTG GGCAGACAAG
    TTTCAGATGT ATGTCACATA TTGCAAAAAT AAGCCTGATT CTACTCAGCT GATATTAGAG
    CATGCAGGAT CCTACTTTGA TGAAATACAA CAACGGCATG GATTAGCCAA TTCCATCTCT
    TCCTACCTTA TTAAGCCAGT TCAGCGAATA ACGAAGTATC AACTTCTTTT AAAAGAACTA
    CTTACTTGCT GTGAGGAAGG AAAGGGAGAG ATAAAAGATG GCCTGGAGGT GATGCTCAGT
    GTGCCAAAAC GAGCCAATGA TGCCATGCAC CTCAGCATGC TGGAAGGGTT TGATGAAAAT
    ATTGAGTCTC AGGGAGAACT CATTCTACAA GAATCCTTCC AAGTGTGGGA CCCAAAAACC
    TTAATTCGAA AGGGTCGAGA ACGGCATCTC TTCCTTTTTG AAATGTCCTT AGTATTTAGT
    AAAGAAGTGA AAGATTCCAG TGGCAGAAGC AAGTACCTTT ATAAAAGCAA ATTGTTTACC
    TCAGAGTTGG GTGTCACAGA ACACGTTGAA GGAGATCCTT GCAAATTTGC ACTGTGGGTG
    GGGAGGACAC CAACCTCAGA TAATAAAATC GTCCTTAAGG CTTCCAGTAT AGAAAACAAG
    CAGGACTGGA TAAAGCACAT CCGCGAGGTC ATACAGGAAA GGACCATTCA CCTGAAAGGA
    GCCCTGAAGG AGCCCATTCA CATTCCCAAA ACGGCGCCGG CCACCAGACA GAAGGGCAGG
    AGGGATGGAG AGGATCTGGA TAGCCAGGGC GATGGGAGCA GCCAGCCTGA CACAATCTCT
    ATCGCCTCGA GGACGTCTCA GAACACACTG GATAGTGATA AGCTCTCTGG CGGCTGCGAG
    CTGACAGTGG TGATCCACGA CTTTACCGCC TGCAACAGCA CAGAGCTGAC CATCCGCCGT
    GGCCAGACCG TGGAGGTTTT GGAGCGGCCC CACGACAAGC CTGACTGGTG TCTGGTGCGG
    ACCACCGACC GGTCCCCTGC TGCAGAAGGC CTGGTTCCCT GTGGCTCTCT CTGTATCGCC
    CACTCGAGAA GTAGCATGGA AATGGAGGGT ATCTTCAACC ACAAAGACTC GCTGTCCGTC
    TCCAGTAACG ATGCCAGCCC GCCGGCGTCC GTGGCCTCCC TCCAGCCCCA CCTGATGGGG
    GCCCAGAGCT CGCCAGGCCC CAAGCGCCCA GGGAACACTC TGCGCAAGTG GCTGACGAGC
    CCGGTGCGGC GGCTGAGCAG CGGGAAGGCG GACGGGCACG TGAAGAAGCT GGCGCACAAG
    CACAAGAAGA GCAGGGAGGT CCGCAAGAGC ACCGACGCGG GCTCGCAGAA GGACTCGGAC
    GACAGCGCGG CCACCCCGCA GGACGAGACT GTCGAGGAGA GAGGCCGGAA TGAAGGCCTG
    AGCAGTGGCA CACTCTCGAA GTCGTCATCG TCGGGCATGC AAAGCTGTGG AGAGGAAGAA
    GGGGAGGAGG GGGCCGACGC CGTGCCTCTC CCCCCGCCCA TGGCCATCCA GCAGCACAGC
    CTCCTCCAGC CGGATTCACA GGACGACAAG GCGTCTTCTC GGCTATTAGT TCGCCCCACC
    AGCTCTGAAA CGCCAAGCGC AGCCGAGCTC GTGAGTGCAA TTGAAGAACT CGTGAAAAGC
    AAGATGGCCT TGGAGGACCG TCCCAGCTCA CTCCTTGTTG ACCAGGGAGA CAGCAGCAGC
    CCCTCCTTCA ACCCTTCAGA CAACTCCCTC CTGTCCTCCT CCTCGCCCAT TGATGAGATG
    GAAGAAAGGA AATCCAGCTC TTTAAAGAGA CGGCACTATG TTTTGCAAGA ACTAGTGGAG
    ACAGAACGTG ACTATGTGCG AGACCTCGGC TATGTAGTTG AGGGCTACAT GGCTCTCATG
    AAAGAAGATG GTGTTCCTGA TGACATGAAA GGAAAGGACA AAATTGTATT TGGCAACATC
    CATCAGATTT ATGACTGGCA CAGAGACTTT TTTTTAGGAG AGTTGGAGAA GTGCCTTGAA
    GATCCAGAAA AACTAGGATC CCTTTTTGTT AAACACGAAA GAAGGTTGCA CATGTACATA
    GTTTATTGTC AAAATAAACC AAAGTCTGAG CACATTGTCT CAGAATACAT TGATACCTTT
    TTTGAGGACT TAAAGCAGCG TCTGGGCCAC AGGTTGCAGC TCACAGATCT GTTGATCAAA
    CCAGTGCAGC GGATTATGAA GTACCAGCTG CTCCTGAAGG ACTTCCTCAA GTATTCCAAA
    AAAGCTAGCC TGGATACATC AGAATTAGAG AGAGCTGTGG AAGTCATGTG CATAGTCCCC
    AAGCGGTGCA ACGACATGAT GAACGTGGGG CGGCTGCAAG GATTTGATGG TAAAATTGTT
    GCCCAGGGCA AGCTACTCTT GCAGGACACA TTCTTGGTCA CAGACCAGGA CTCGGGACTT
    CTGCCTCGCT GTAAAGAGAG GCGAGTTTTC CTCTTTGAGC AAATCGTCAT TTTCAGTGAA
    CCACTTGATA AAAAAAAGGG CTTCTCGATG CCAGGATTCC TGTTTAAGAA CAGTATCAAG
    GTGAGTTGCC TTTGCCTGGA GGAAAATGTG GAAAATGATC CATGTAAATT TGCTTTGACG
    TCGAGGACGG GTGGTGTGGT GGAGACCTTC ATTTTGCATT CATCCAGTCC AAGTGTCCGG
    CAAACATGGA TCCATGAAAT CAACCAAATT TTAGAAAACC AGCGCAATTT TTTAAATGCC
    TTGACATCAC CAATCGAGTA TCAGAGGAAC CACAGTGGGG GCGGCGGCGG GGGCAGTGGC
    GGGGGCGGCG GCAGCGGAGG CAGCGGCGGC GGGGCTGGCA GCGGGGCCCC CGGCGGCGGA
    GGCAGCAACC CCAGCGGCGG CCCTGGCAGC TGCAGCGGCC TCCCCAGCTC GAGCAGAAGC
    CGACCCTCCC GGATCCCCCA GCCTGTCAGA CACCACTCCC CCGTGCTGGT CTCCTCTGCA
    GCCTCGGGCC AGGCAGAGGC AGACAAGATG TCAGGTACGT CCACTCCCGG CCCTGCACTG
    GCCCCTCCCG GCAGCAGCCC CGCCCCGGAG CCCGGCCCCA GCCAGCCTGG CAGGCGCGCC
    CCCGAGCCCG GAGGCCCCGA GCGAGAAGCC GAGCTCATCC CCAGGATGAA GGTGATTGAG
    AGCCCCCGGA GGTGCTCGGG GGGCGCGGCG GGCGCCGGCC GCGATGCGGA CACCAAGGAG
    GCGCGGGCCC CCCCGGAGGA CAGCCGCTCG CGGAGCAGCC CGGGCTCGCT GCCCCTGGCA
    AAGCCCAGGC CTGGGGCCAC CTCGCCTCTG AACTCTCCCG TGGCCGCCGC ATTACCTCCT
    CCAATCGGCA AGGAGCCCTG TCCCCCCAGC AGCCCCCTGC AGAAGGGGGG CTCCTTCTGG
    AGCTCCATCC CCGCCTCCCC CGCCAGCCGG CCAGGCTCCT TCACCTTCCC GGGGGACAGT
    GACTCCCTCC AGCGGCAGGC CCAGCGCCAC GCGGCCCCCA GCAAGGACAC AGACCGCATG
    AGCACCTGCT CGTCGGCCAG CGAGCAGTCG GTACAGTCCA CCCAGAGCAA CGGGAGTGAA
    AGTAGCAGCA GTAGCAACAT CTCCACCATG TTGGTGACAC ACGATTACAC GGCAGTGAAG
    GAGGATGAGA TAAACGTCTA TCAAGGAGAG GTCGTTCAAA TTCTGGCCAG CAATCAACAG
    AACATGTTTC TGGTGTTCCG AGCCGCCACT GACCAGTGCC CCGCAGCCGA GGGCTGGATT
    CCAGGCTTCG TCCTGGGGCA TACCAGTGCA GTCATCATGG AGAACCCCGA TGGGACTCTC
    AAGAAGTCAA CATCTTGGCA CACAGCACTC CGTTTAAGGA AAAAATCTGA GAAAAAAGAT
    AAAGACGGCA AGCGGGAAGG CAAGTTAGAG AATGGTTATC GGAAGTCACG GGAGGGGCTC
    AGCAGCAAGG TATCTGTGAA GCTTCTCAAT CCCAACTACA TATATGACGT TCCCCCAGAA
    TTCGTCATTC CCTTGAGTGA GGTCACGTGT GAGACAGGGG AGACCGTTGT TTTTAGATGT
    CGGGTTTGTG GCCGCCCCAA GGCCTCAATC ACCTGGAAGG GCCCTGAGCA CAACACCCTG
    AACAACGATG GTCACTACAG CATCTCCTAC AGTGACTTGG GAGAGGCCGC TTTGAAGATC
    GTCGGCGTGA CCACAGAAGA TGATGGCATC TACACATGCA TCGCTGTGAA CGACATGGGT
    TCAGCCTCGT CTTCGGCCAG TCTGAGGGTG CTGGGTCCAG GGAGTGATGG GATCATGGTG
    ACCTGGAAAG ACAACTTTGA CTCCTTCTAC AGCGAAGTGG CTGAGCTTGG CAGGGGCAGA
    TTCTCTGTCG TTAAGAAATG TGATCAGAAA GGAACCAAAC GAGCAGTGGC CACTAAATTT
    GTGAACAAGA AGTTGATGAA GCGTGACCAG GTCACCCATG AACTTGGAAT CCTGCAGAAC
    CTCCAGCACC CGCTGCTTAT TGGTCTCCTT GATACCTTCG AGACCCCCAC CAGCTACGTC
    CTGGTGTTAG AAATGGCTGA CCAGGGGCGT CTCCTGGACT GTGTGGTGCG ATGGGGAGAC
    CTCACTGAAG GGAAGATCAG GGCGTATCTG GGGGAGGTTC TGGAAGCCGT CCAATACCTT
    CACAACTGCA GGATAGCACA CCTGGACCTA AAGCCTGAAA ACATTCTGGT GGATCAGAGT
    CTGGCCAAGC CCACGATCAA ACTGGCCGAT TTTGGAGATG CTGTCCAACT CAACACGACC
    TACTACATCC ATCAGTTACT GGGGAACCCC GAGTTTGCAG CCCCCGAAAT CATCCTTGGG
    AACCCCATCT CCCTGACCTC GGATACGTGG AGTGTCGGAG TGCTCGCTTA CGTGCTTCTC
    AGCGGCGTGT CCCCCTTCCT CGACGACAGT GTGGAGGAGA CCTGCCTGAA CATTTGCCGA
    CTAGACTTTA GTTTCCCGGA GGACTGCTTT AAAGGTGTGA GCCAGAAGGC CAAGGAGTTC
    GTGTGTTTCC TTCTCCAGGA GGACCCTGCC AGGCGTCCCT CGGCTGCGCT GGCCCTGCAG
    GAGCAGTGGC TGCAGGCGGG CCACGGCGGT GGCAAAGGCG CGGGCGTCCT CGACACGTCC
    AGACTGACTT CCTTCATTGA GCGGCGCAAA CACCAGAACG ATGTTCGACC TATCCGTAGC
    GTTAAAAACT TCTTACAGAG CAGGCTTTTG CCTAGAGTTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.