ITSN1 cDNA ORF clone, Canis lupus familiaris(Dog)

The following ITSN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ITSN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe44462 XM_014109749.2
Latest version!
Canis lupus familiaris intersectin 1 (ITSN1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe283918 XM_022413235.1
Latest version!
Canis lupus familiaris intersectin 1 (ITSN1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe283919 XM_022413236.1
Latest version!
Canis lupus familiaris intersectin 1 (ITSN1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe283920 XM_022413237.1
Latest version!
Canis lupus familiaris intersectin 1 (ITSN1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe44461 XM_014109748.2
Latest version!
Canis lupus familiaris intersectin 1 (ITSN1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe29391 XM_005638880.3
Latest version!
Canis lupus familiaris intersectin 1 (ITSN1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe29392 XM_014109747.2
Latest version!
Canis lupus familiaris intersectin 1 (ITSN1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
View Old Accession Versions >>
OCe44462 XM_014109749.1 Canis lupus familiaris intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe44461 XM_014109748.1 Canis lupus familiaris intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe29391 XM_005638880.2 Canis lupus familiaris intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe29392 XM_014109747.1 Canis lupus familiaris intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe44462
    Clone ID Related Accession (Same CDS sequence) XM_014109749.1 , XM_014109749.2
    Accession Version XM_014109749.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3663bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product intersectin-1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCTCAGT TTCCAACACC TTTTGGAGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAA 
    GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG
    ATATGGGCGC TAGCTGACAT GAACAATGAT GGACGAATGG ATCAAGTGGA GTTTTCCATA
    GCTATGAAAC TCATCAAACT CAAGCTGCAA GGATATCAAC TTCCTTCTGC ACTTCCCCCC
    GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CCTTTGGTAT AGGAGGTATT
    GCCAGCATGC CACCACTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTC
    GGAATGTCTC CACCTCTAGT ATCTTCTGTT CCTACAGCAG CAGTGCCTCC CCTGGCTAAC
    GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA
    AAGAGCTCTT CCTTCAGTAG ATCTGGTCCC GGGTCACAAT TAAACACTAA GTTACAGAAG
    GCACAGTCAT TTGATGTGGC TAGTGTCCCT CCCGTGGCAG AATGGGCTGT CCCTCAGTCA
    TCCAGACTTA AATACAGACA GTTATTCAAC AGTCATGACA AAACTATGAG TGGACACTTA
    ACAGGTCCAC AAGCAAGGAC TATTCTTATG CAATCAAGTT TGCCACAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC
    TTGGCTATGC ACCTAATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT
    CCAGAGTACA TTCCACCTTC CTTTAGACGA GTTCGATCTG GCAGTGGTAT ATCAGTCATC
    AGCTCAACAG CAGTAGATCA GAGGTTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA
    CAACTAGAAA AGAAATTACC TGTAACCTTT GAAGATAAGA AGCGTGAGAA TTTTGAACGT
    GGCAATCTGG AACTCGAGAA ACGGAGACAA GCGCTCTTGG AGCAGCAGCG CAAAGAGCAA
    GAGCGCCTGG CACAGCTTGA ACGTGCAGAG CAAGAACGGA AAGAGCGGGA GCGCCAGGAG
    CAAGAGCGCA AAAGGCAACT GGAGTTGGAG AAACAACTGG AAAAGCAACG GGAGCTAGAA
    CGTCAGAGAG AAGAGGAGAG GAGGAAAGAA ATTGAGAGAC GAGAGGCTGC AAAGAGAGAA
    CTTGAAAGAC AGCGGCAACT GGAATGGGAA CGAAATCGAA GACAAGAGCT ACTCAATCAG
    AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAGAAGAC TCTAGAATTT
    GAATTAGAAG CTCTTAATGA TAAAAAGCAT CAACTAGAAG GAAAACTTCA AGATATCAGG
    TGTCGATTGA CCACCCAACG GCAAGAAATT GAGAGTACAA ATAAATCCAG AGAGTTGAGA
    ATTGCTGAAA TCACCCATCT ACAGCAACAA TTACAGGAAG CCCAGCAAAT GCTTGGAAGA
    CTTATTCCAG AAAAGCAGAT ACTCAATGAC CAATTAAAAC AAGTTCAACA GAACAGTTTG
    CACAGAGATT CACTGCTTAC ACTCAAAAGA GCGTTAGAAG CAAAAGAACT AGCTCGGCAG
    CAGCTACGCG ATCAACTGGA TGAAGTTGAG AAAGAAACCA GATCAAAACT ACAGGAGATT
    GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ATAGTAAGCA ACAACTCCAG
    AAACAAAAGT CCATAGAGGC CGAACGTCTG AAACAGAAAG AACAAGAGCG AAAAACCATA
    GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAGAGGGA CAAGCAGTGG
    ATTGAGCATG TGCAGCAGGA CGATGAGCAT CAGCGGCCAA GGAAGATCCA TGATGAAGAA
    AAACTCCGAA GGGAAGAGAG TGTCAAAAAG AAGGACAGTG AGGAAAAAAG CAAACTGGAC
    ACGCAAGACA AGATGAGTCG GCTTTTCCAC CAGCATCAAG AGCCAGCCAA GCCAGCTGTC
    CAGGCACCCT GGTCCACCAC AGAAAAAGGC CCACTTACAA TTTCTGCACA GGAGAACGTA
    AAGGTGGTGT ATTACCGAGC GCTCTATCCC TTCGAATCCA GAAGTCATGA TGAGATCACG
    ATCCAGCCTG GAGACATAGT AATGGTTAAA GGGGAATGGG TGGATGAAAG CCAAACTGGA
    GAACCGGGCT GGCTTGGAGG AGAATTAAAA GGAAAGACGG GATGGTTCCC TGCTAACTAC
    GCAGAGAAAA TCCCAGAAAA CGAGGTGCCC GCTCCAGTCA AACCCGGGCC CGACCTCACC
    TCTGCCCCTG CGCCCAAACT GGCCGTGCGT GAGACCCCCG CTCCGGTGGC AGTGAGCGCT
    CCGGAGCCCT CCACGACCCC CAACAGCTGG GCTGACTTCA GCTCCACGTG GCCCACCAGC
    ACGAATGAGA AGCCAGAAAC CGACAACTGG GACGCATGGG CAGCCCAGCC CTCACTCACT
    GTTCCCAGTG CTGGCCAGCT AAGGCAGAGA TCAGCGTTTA CTCCAGCCAC GGCCACTGGC
    TCCTCTCCAT CTCCCGTTTT AGGCCAGGGT GAGAAGGTGG AAGGGCTACA AGCACAAGCC
    CTGTATCCTT GGAGAGCCAA AAAAGACAAC CACTTAAATT TTAACAAAAA TGACGTCATC
    ACCGTCCTGG AACAGCAAGA CATGTGGTGG TTTGGAGAAG TCCAAGGTCA GAAGGGTTGG
    TTCCCCAAGT CTTACGTGAA ACTCATTTCA GGGCCCATAA GGAAATCCAC AAGCATGGAT
    TCTGGTTCTT CAGAAAGTCC CGCTAGTCTA AAGAGAGTAG CTTCCCCAGC AGCCAAGCCG
    GCCATTTCGG GAGAAGAATT TATTGCCATG TACACTTACG AGAGTTCTGA GCAAGGCGAT
    TTAACGTTTC AGCAAGGGGA TGTGATTTTG GTTACCAAGA AAGATGGTGA CTGGTGGACA
    GGAGCAGTGG GCGACAAGTC CGGAGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCA
    GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATTGCGCAG
    GTTATCGCCT GCTACACAGC TACTGGTCCC GAGCAGCTCA CCCTGGCCCC TGGTCAGCTG
    ATTTTGATCC GAAAAAAGAA CCCAGGCGGT TGGTGGGAAG GAGAGCTGCA AGCCCGTGGG
    AAGAAGCGCC AGATAGGCTG GTTCCCCGCG AATTATGTAA AACTTTTGAG CCCTGGCACG
    AGCAAAATCA CTCCAACGGA GCCACCTAAG CCCACGGCGT TCCCGGCAGT GTGCCAGGTG
    ATCGGCGTGT ACGACTACAG CGCCCAGAAC GACGACGAGC TGGCCTTCAG CAAGGGCCAG
    ATCATCAACG TCCTCAACAA AGACGACCCG GACTGGTGGA AGGGCGAGGT CAACGGGCAA
    GTGGGGCTCT TCCCCTCCAA CTACGTGAAG CTGACCACGG ACATGGACCC AAGCCAGCAA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965224.1
    CDS290..3952
    Translation

    Target ORF information:

    RefSeq Version XM_014109749.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109749.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCTCAGT TTCCAACACC TTTTGGAGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAA 
    GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG
    ATATGGGCGC TAGCTGACAT GAACAATGAT GGACGAATGG ATCAAGTGGA GTTTTCCATA
    GCTATGAAAC TCATCAAACT CAAGCTGCAA GGATATCAAC TTCCTTCTGC ACTTCCCCCC
    GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CCTTTGGTAT AGGAGGTATT
    GCCAGCATGC CACCACTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTC
    GGAATGTCTC CACCTCTAGT ATCTTCTGTT CCTACAGCAG CAGTGCCTCC CCTGGCTAAC
    GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA
    AAGAGCTCTT CCTTCAGTAG ATCTGGTCCC GGGTCACAAT TAAACACTAA GTTACAGAAG
    GCACAGTCAT TTGATGTGGC TAGTGTCCCT CCCGTGGCAG AATGGGCTGT CCCTCAGTCA
    TCCAGACTTA AATACAGACA GTTATTCAAC AGTCATGACA AAACTATGAG TGGACACTTA
    ACAGGTCCAC AAGCAAGGAC TATTCTTATG CAATCAAGTT TGCCACAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC
    TTGGCTATGC ACCTAATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT
    CCAGAGTACA TTCCACCTTC CTTTAGACGA GTTCGATCTG GCAGTGGTAT ATCAGTCATC
    AGCTCAACAG CAGTAGATCA GAGGTTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA
    CAACTAGAAA AGAAATTACC TGTAACCTTT GAAGATAAGA AGCGTGAGAA TTTTGAACGT
    GGCAATCTGG AACTCGAGAA ACGGAGACAA GCGCTCTTGG AGCAGCAGCG CAAAGAGCAA
    GAGCGCCTGG CACAGCTTGA ACGTGCAGAG CAAGAACGGA AAGAGCGGGA GCGCCAGGAG
    CAAGAGCGCA AAAGGCAACT GGAGTTGGAG AAACAACTGG AAAAGCAACG GGAGCTAGAA
    CGTCAGAGAG AAGAGGAGAG GAGGAAAGAA ATTGAGAGAC GAGAGGCTGC AAAGAGAGAA
    CTTGAAAGAC AGCGGCAACT GGAATGGGAA CGAAATCGAA GACAAGAGCT ACTCAATCAG
    AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAGAAGAC TCTAGAATTT
    GAATTAGAAG CTCTTAATGA TAAAAAGCAT CAACTAGAAG GAAAACTTCA AGATATCAGG
    TGTCGATTGA CCACCCAACG GCAAGAAATT GAGAGTACAA ATAAATCCAG AGAGTTGAGA
    ATTGCTGAAA TCACCCATCT ACAGCAACAA TTACAGGAAG CCCAGCAAAT GCTTGGAAGA
    CTTATTCCAG AAAAGCAGAT ACTCAATGAC CAATTAAAAC AAGTTCAACA GAACAGTTTG
    CACAGAGATT CACTGCTTAC ACTCAAAAGA GCGTTAGAAG CAAAAGAACT AGCTCGGCAG
    CAGCTACGCG ATCAACTGGA TGAAGTTGAG AAAGAAACCA GATCAAAACT ACAGGAGATT
    GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ATAGTAAGCA ACAACTCCAG
    AAACAAAAGT CCATAGAGGC CGAACGTCTG AAACAGAAAG AACAAGAGCG AAAAACCATA
    GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAGAGGGA CAAGCAGTGG
    ATTGAGCATG TGCAGCAGGA CGATGAGCAT CAGCGGCCAA GGAAGATCCA TGATGAAGAA
    AAACTCCGAA GGGAAGAGAG TGTCAAAAAG AAGGACAGTG AGGAAAAAAG CAAACTGGAC
    ACGCAAGACA AGATGAGTCG GCTTTTCCAC CAGCATCAAG AGCCAGCCAA GCCAGCTGTC
    CAGGCACCCT GGTCCACCAC AGAAAAAGGC CCACTTACAA TTTCTGCACA GGAGAACGTA
    AAGGTGGTGT ATTACCGAGC GCTCTATCCC TTCGAATCCA GAAGTCATGA TGAGATCACG
    ATCCAGCCTG GAGACATAGT AATGGTTAAA GGGGAATGGG TGGATGAAAG CCAAACTGGA
    GAACCGGGCT GGCTTGGAGG AGAATTAAAA GGAAAGACGG GATGGTTCCC TGCTAACTAC
    GCAGAGAAAA TCCCAGAAAA CGAGGTGCCC GCTCCAGTCA AACCCGGGCC CGACCTCACC
    TCTGCCCCTG CGCCCAAACT GGCCGTGCGT GAGACCCCCG CTCCGGTGGC AGTGAGCGCT
    CCGGAGCCCT CCACGACCCC CAACAGCTGG GCTGACTTCA GCTCCACGTG GCCCACCAGC
    ACGAATGAGA AGCCAGAAAC CGACAACTGG GACGCATGGG CAGCCCAGCC CTCACTCACT
    GTTCCCAGTG CTGGCCAGCT AAGGCAGAGA TCAGCGTTTA CTCCAGCCAC GGCCACTGGC
    TCCTCTCCAT CTCCCGTTTT AGGCCAGGGT GAGAAGGTGG AAGGGCTACA AGCACAAGCC
    CTGTATCCTT GGAGAGCCAA AAAAGACAAC CACTTAAATT TTAACAAAAA TGACGTCATC
    ACCGTCCTGG AACAGCAAGA CATGTGGTGG TTTGGAGAAG TCCAAGGTCA GAAGGGTTGG
    TTCCCCAAGT CTTACGTGAA ACTCATTTCA GGGCCCATAA GGAAATCCAC AAGCATGGAT
    TCTGGTTCTT CAGAAAGTCC CGCTAGTCTA AAGAGAGTAG CTTCCCCAGC AGCCAAGCCG
    GCCATTTCGG GAGAAGAATT TATTGCCATG TACACTTACG AGAGTTCTGA GCAAGGCGAT
    TTAACGTTTC AGCAAGGGGA TGTGATTTTG GTTACCAAGA AAGATGGTGA CTGGTGGACA
    GGAGCAGTGG GCGACAAGTC CGGAGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCA
    GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATTGCGCAG
    GTTATCGCCT GCTACACAGC TACTGGTCCC GAGCAGCTCA CCCTGGCCCC TGGTCAGCTG
    ATTTTGATCC GAAAAAAGAA CCCAGGCGGT TGGTGGGAAG GAGAGCTGCA AGCCCGTGGG
    AAGAAGCGCC AGATAGGCTG GTTCCCCGCG AATTATGTAA AACTTTTGAG CCCTGGCACG
    AGCAAAATCA CTCCAACGGA GCCACCTAAG CCCACGGCGT TCCCGGCAGT GTGCCAGGTG
    ATCGGCGTGT ACGACTACAG CGCCCAGAAC GACGACGAGC TGGCCTTCAG CAAGGGCCAG
    ATCATCAACG TCCTCAACAA AGACGACCCG GACTGGTGGA AGGGCGAGGT CAACGGGCAA
    GTGGGGCTCT TCCCCTCCAA CTACGTGAAG CTGACCACGG ACATGGACCC AAGCCAGCAA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44462
    Clone ID Related Accession (Same CDS sequence) XM_014109749.1 , XM_014109749.2
    Accession Version XM_014109749.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3663bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product intersectin-1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109749.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCTCAGT TTCCAACACC TTTTGGAGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAA 
    GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG
    ATATGGGCGC TAGCTGACAT GAACAATGAT GGACGAATGG ATCAAGTGGA GTTTTCCATA
    GCTATGAAAC TCATCAAACT CAAGCTGCAA GGATATCAAC TTCCTTCTGC ACTTCCCCCC
    GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CCTTTGGTAT AGGAGGTATT
    GCCAGCATGC CACCACTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTC
    GGAATGTCTC CACCTCTAGT ATCTTCTGTT CCTACAGCAG CAGTGCCTCC CCTGGCTAAC
    GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA
    AAGAGCTCTT CCTTCAGTAG ATCTGGTCCC GGGTCACAAT TAAACACTAA GTTACAGAAG
    GCACAGTCAT TTGATGTGGC TAGTGTCCCT CCCGTGGCAG AATGGGCTGT CCCTCAGTCA
    TCCAGACTTA AATACAGACA GTTATTCAAC AGTCATGACA AAACTATGAG TGGACACTTA
    ACAGGTCCAC AAGCAAGGAC TATTCTTATG CAATCAAGTT TGCCACAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC
    TTGGCTATGC ACCTAATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT
    CCAGAGTACA TTCCACCTTC CTTTAGACGA GTTCGATCTG GCAGTGGTAT ATCAGTCATC
    AGCTCAACAG CAGTAGATCA GAGGTTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA
    CAACTAGAAA AGAAATTACC TGTAACCTTT GAAGATAAGA AGCGTGAGAA TTTTGAACGT
    GGCAATCTGG AACTCGAGAA ACGGAGACAA GCGCTCTTGG AGCAGCAGCG CAAAGAGCAA
    GAGCGCCTGG CACAGCTTGA ACGTGCAGAG CAAGAACGGA AAGAGCGGGA GCGCCAGGAG
    CAAGAGCGCA AAAGGCAACT GGAGTTGGAG AAACAACTGG AAAAGCAACG GGAGCTAGAA
    CGTCAGAGAG AAGAGGAGAG GAGGAAAGAA ATTGAGAGAC GAGAGGCTGC AAAGAGAGAA
    CTTGAAAGAC AGCGGCAACT GGAATGGGAA CGAAATCGAA GACAAGAGCT ACTCAATCAG
    AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAGAAGAC TCTAGAATTT
    GAATTAGAAG CTCTTAATGA TAAAAAGCAT CAACTAGAAG GAAAACTTCA AGATATCAGG
    TGTCGATTGA CCACCCAACG GCAAGAAATT GAGAGTACAA ATAAATCCAG AGAGTTGAGA
    ATTGCTGAAA TCACCCATCT ACAGCAACAA TTACAGGAAG CCCAGCAAAT GCTTGGAAGA
    CTTATTCCAG AAAAGCAGAT ACTCAATGAC CAATTAAAAC AAGTTCAACA GAACAGTTTG
    CACAGAGATT CACTGCTTAC ACTCAAAAGA GCGTTAGAAG CAAAAGAACT AGCTCGGCAG
    CAGCTACGCG ATCAACTGGA TGAAGTTGAG AAAGAAACCA GATCAAAACT ACAGGAGATT
    GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ATAGTAAGCA ACAACTCCAG
    AAACAAAAGT CCATAGAGGC CGAACGTCTG AAACAGAAAG AACAAGAGCG AAAAACCATA
    GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAGAGGGA CAAGCAGTGG
    ATTGAGCATG TGCAGCAGGA CGATGAGCAT CAGCGGCCAA GGAAGATCCA TGATGAAGAA
    AAACTCCGAA GGGAAGAGAG TGTCAAAAAG AAGGACAGTG AGGAAAAAAG CAAACTGGAC
    ACGCAAGACA AGATGAGTCG GCTTTTCCAC CAGCATCAAG AGCCAGCCAA GCCAGCTGTC
    CAGGCACCCT GGTCCACCAC AGAAAAAGGC CCACTTACAA TTTCTGCACA GGAGAACGTA
    AAGGTGGTGT ATTACCGAGC GCTCTATCCC TTCGAATCCA GAAGTCATGA TGAGATCACG
    ATCCAGCCTG GAGACATAGT AATGGTTAAA GGGGAATGGG TGGATGAAAG CCAAACTGGA
    GAACCGGGCT GGCTTGGAGG AGAATTAAAA GGAAAGACGG GATGGTTCCC TGCTAACTAC
    GCAGAGAAAA TCCCAGAAAA CGAGGTGCCC GCTCCAGTCA AACCCGGGCC CGACCTCACC
    TCTGCCCCTG CGCCCAAACT GGCCGTGCGT GAGACCCCCG CTCCGGTGGC AGTGAGCGCT
    CCGGAGCCCT CCACGACCCC CAACAGCTGG GCTGACTTCA GCTCCACGTG GCCCACCAGC
    ACGAATGAGA AGCCAGAAAC CGACAACTGG GACGCATGGG CAGCCCAGCC CTCACTCACT
    GTTCCCAGTG CTGGCCAGCT AAGGCAGAGA TCAGCGTTTA CTCCAGCCAC GGCCACTGGC
    TCCTCTCCAT CTCCCGTTTT AGGCCAGGGT GAGAAGGTGG AAGGGCTACA AGCACAAGCC
    CTGTATCCTT GGAGAGCCAA AAAAGACAAC CACTTAAATT TTAACAAAAA TGACGTCATC
    ACCGTCCTGG AACAGCAAGA CATGTGGTGG TTTGGAGAAG TCCAAGGTCA GAAGGGTTGG
    TTCCCCAAGT CTTACGTGAA ACTCATTTCA GGGCCCATAA GGAAATCCAC AAGCATGGAT
    TCTGGTTCTT CAGAAAGTCC CGCTAGTCTA AAGAGAGTAG CTTCCCCAGC AGCCAAGCCG
    GCCATTTCGG GAGAAGAATT TATTGCCATG TACACTTACG AGAGTTCTGA GCAAGGCGAT
    TTAACGTTTC AGCAAGGGGA TGTGATTTTG GTTACCAAGA AAGATGGTGA CTGGTGGACA
    GGAGCAGTGG GCGACAAGTC CGGAGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCA
    GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATTGCGCAG
    GTTATCGCCT GCTACACAGC TACTGGTCCC GAGCAGCTCA CCCTGGCCCC TGGTCAGCTG
    ATTTTGATCC GAAAAAAGAA CCCAGGCGGT TGGTGGGAAG GAGAGCTGCA AGCCCGTGGG
    AAGAAGCGCC AGATAGGCTG GTTCCCCGCG AATTATGTAA AACTTTTGAG CCCTGGCACG
    AGCAAAATCA CTCCAACGGA GCCACCTAAG CCCACGGCGT TCCCGGCAGT GTGCCAGGTG
    ATCGGCGTGT ACGACTACAG CGCCCAGAAC GACGACGAGC TGGCCTTCAG CAAGGGCCAG
    ATCATCAACG TCCTCAACAA AGACGACCCG GACTGGTGGA AGGGCGAGGT CAACGGGCAA
    GTGGGGCTCT TCCCCTCCAA CTACGTGAAG CTGACCACGG ACATGGACCC AAGCCAGCAA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965224.1
    CDS231..3893
    Translation

    Target ORF information:

    RefSeq Version XM_014109749.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris intersectin 1 (ITSN1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109749.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCTCAGT TTCCAACACC TTTTGGAGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAA 
    GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG
    ATATGGGCGC TAGCTGACAT GAACAATGAT GGACGAATGG ATCAAGTGGA GTTTTCCATA
    GCTATGAAAC TCATCAAACT CAAGCTGCAA GGATATCAAC TTCCTTCTGC ACTTCCCCCC
    GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CCTTTGGTAT AGGAGGTATT
    GCCAGCATGC CACCACTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTC
    GGAATGTCTC CACCTCTAGT ATCTTCTGTT CCTACAGCAG CAGTGCCTCC CCTGGCTAAC
    GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA
    AAGAGCTCTT CCTTCAGTAG ATCTGGTCCC GGGTCACAAT TAAACACTAA GTTACAGAAG
    GCACAGTCAT TTGATGTGGC TAGTGTCCCT CCCGTGGCAG AATGGGCTGT CCCTCAGTCA
    TCCAGACTTA AATACAGACA GTTATTCAAC AGTCATGACA AAACTATGAG TGGACACTTA
    ACAGGTCCAC AAGCAAGGAC TATTCTTATG CAATCAAGTT TGCCACAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC
    TTGGCTATGC ACCTAATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT
    CCAGAGTACA TTCCACCTTC CTTTAGACGA GTTCGATCTG GCAGTGGTAT ATCAGTCATC
    AGCTCAACAG CAGTAGATCA GAGGTTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA
    CAACTAGAAA AGAAATTACC TGTAACCTTT GAAGATAAGA AGCGTGAGAA TTTTGAACGT
    GGCAATCTGG AACTCGAGAA ACGGAGACAA GCGCTCTTGG AGCAGCAGCG CAAAGAGCAA
    GAGCGCCTGG CACAGCTTGA ACGTGCAGAG CAAGAACGGA AAGAGCGGGA GCGCCAGGAG
    CAAGAGCGCA AAAGGCAACT GGAGTTGGAG AAACAACTGG AAAAGCAACG GGAGCTAGAA
    CGTCAGAGAG AAGAGGAGAG GAGGAAAGAA ATTGAGAGAC GAGAGGCTGC AAAGAGAGAA
    CTTGAAAGAC AGCGGCAACT GGAATGGGAA CGAAATCGAA GACAAGAGCT ACTCAATCAG
    AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAGAAGAC TCTAGAATTT
    GAATTAGAAG CTCTTAATGA TAAAAAGCAT CAACTAGAAG GAAAACTTCA AGATATCAGG
    TGTCGATTGA CCACCCAACG GCAAGAAATT GAGAGTACAA ATAAATCCAG AGAGTTGAGA
    ATTGCTGAAA TCACCCATCT ACAGCAACAA TTACAGGAAG CCCAGCAAAT GCTTGGAAGA
    CTTATTCCAG AAAAGCAGAT ACTCAATGAC CAATTAAAAC AAGTTCAACA GAACAGTTTG
    CACAGAGATT CACTGCTTAC ACTCAAAAGA GCGTTAGAAG CAAAAGAACT AGCTCGGCAG
    CAGCTACGCG ATCAACTGGA TGAAGTTGAG AAAGAAACCA GATCAAAACT ACAGGAGATT
    GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ATAGTAAGCA ACAACTCCAG
    AAACAAAAGT CCATAGAGGC CGAACGTCTG AAACAGAAAG AACAAGAGCG AAAAACCATA
    GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAGAGGGA CAAGCAGTGG
    ATTGAGCATG TGCAGCAGGA CGATGAGCAT CAGCGGCCAA GGAAGATCCA TGATGAAGAA
    AAACTCCGAA GGGAAGAGAG TGTCAAAAAG AAGGACAGTG AGGAAAAAAG CAAACTGGAC
    ACGCAAGACA AGATGAGTCG GCTTTTCCAC CAGCATCAAG AGCCAGCCAA GCCAGCTGTC
    CAGGCACCCT GGTCCACCAC AGAAAAAGGC CCACTTACAA TTTCTGCACA GGAGAACGTA
    AAGGTGGTGT ATTACCGAGC GCTCTATCCC TTCGAATCCA GAAGTCATGA TGAGATCACG
    ATCCAGCCTG GAGACATAGT AATGGTTAAA GGGGAATGGG TGGATGAAAG CCAAACTGGA
    GAACCGGGCT GGCTTGGAGG AGAATTAAAA GGAAAGACGG GATGGTTCCC TGCTAACTAC
    GCAGAGAAAA TCCCAGAAAA CGAGGTGCCC GCTCCAGTCA AACCCGGGCC CGACCTCACC
    TCTGCCCCTG CGCCCAAACT GGCCGTGCGT GAGACCCCCG CTCCGGTGGC AGTGAGCGCT
    CCGGAGCCCT CCACGACCCC CAACAGCTGG GCTGACTTCA GCTCCACGTG GCCCACCAGC
    ACGAATGAGA AGCCAGAAAC CGACAACTGG GACGCATGGG CAGCCCAGCC CTCACTCACT
    GTTCCCAGTG CTGGCCAGCT AAGGCAGAGA TCAGCGTTTA CTCCAGCCAC GGCCACTGGC
    TCCTCTCCAT CTCCCGTTTT AGGCCAGGGT GAGAAGGTGG AAGGGCTACA AGCACAAGCC
    CTGTATCCTT GGAGAGCCAA AAAAGACAAC CACTTAAATT TTAACAAAAA TGACGTCATC
    ACCGTCCTGG AACAGCAAGA CATGTGGTGG TTTGGAGAAG TCCAAGGTCA GAAGGGTTGG
    TTCCCCAAGT CTTACGTGAA ACTCATTTCA GGGCCCATAA GGAAATCCAC AAGCATGGAT
    TCTGGTTCTT CAGAAAGTCC CGCTAGTCTA AAGAGAGTAG CTTCCCCAGC AGCCAAGCCG
    GCCATTTCGG GAGAAGAATT TATTGCCATG TACACTTACG AGAGTTCTGA GCAAGGCGAT
    TTAACGTTTC AGCAAGGGGA TGTGATTTTG GTTACCAAGA AAGATGGTGA CTGGTGGACA
    GGAGCAGTGG GCGACAAGTC CGGAGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCA
    GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATTGCGCAG
    GTTATCGCCT GCTACACAGC TACTGGTCCC GAGCAGCTCA CCCTGGCCCC TGGTCAGCTG
    ATTTTGATCC GAAAAAAGAA CCCAGGCGGT TGGTGGGAAG GAGAGCTGCA AGCCCGTGGG
    AAGAAGCGCC AGATAGGCTG GTTCCCCGCG AATTATGTAA AACTTTTGAG CCCTGGCACG
    AGCAAAATCA CTCCAACGGA GCCACCTAAG CCCACGGCGT TCCCGGCAGT GTGCCAGGTG
    ATCGGCGTGT ACGACTACAG CGCCCAGAAC GACGACGAGC TGGCCTTCAG CAAGGGCCAG
    ATCATCAACG TCCTCAACAA AGACGACCCG GACTGGTGGA AGGGCGAGGT CAACGGGCAA
    GTGGGGCTCT TCCCCTCCAA CTACGTGAAG CTGACCACGG ACATGGACCC AAGCCAGCAA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe283918
    Clone ID Related Accession (Same CDS sequence) XM_022413235.1
    Accession Version XM_022413235.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4968bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product intersectin-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAACAATG ATGGACGAAT GGATCAAGTG GAGTTTTCCA TAGCTATGAA ACTCATCAAA 
    CTCAAGCTGC AAGGATATCA ACTTCCTTCT GCACTTCCCC CCGTCATGAA ACAGCAACCA
    GTTGCTATTT CTAGCGCACC AGCCTTTGGT ATAGGAGGTA TTGCCAGCAT GCCACCACTT
    ACAGCTGTTG CTCCAGTGCC AATGGGATCC ATTCCAGTTG TCGGAATGTC TCCACCTCTA
    GTATCTTCTG TTCCTACAGC AGCAGTGCCT CCCCTGGCTA ACGGGGCTCC CCCTGTTATA
    CAACCTCTGC CTGCATTTGC TCATCCTGCA GCCACATTGC CAAAGAGCTC TTCCTTCAGT
    AGATCTGGTC CCGGGTCACA ATTAAACACT AAGTTACAGA AGGCACAGTC ATTTGATGTG
    GCTAGTGTCC CTCCCGTGGC AGAATGGGCT GTCCCTCAGT CATCCAGACT TAAATACAGA
    CAGTTATTCA ACAGTCATGA CAAAACTATG AGTGGACACT TAACAGGTCC ACAAGCAAGG
    ACTATTCTTA TGCAATCAAG TTTGCCACAG GCTCAGCTGG CTTCAATATG GAATCTTTCT
    GACATTGATC AAGATGGAAA ACTCACAGCA GAAGAATTTA TCTTGGCTAT GCACCTAATT
    GATGTAGCTA TGTCTGGCCA ACCACTGCCA CCTGTCCTGC CTCCAGAGTA CATTCCACCT
    TCCTTTAGAC GAGTTCGATC TGGCAGTGGT ATATCAGTCA TCAGCTCAAC AGCAGTAGAT
    CAGAGGTTAC CAGAGGAACC AGTTTTAGAA GATGAACAAC AACAACTAGA AAAGAAATTA
    CCTGTAACCT TTGAAGATAA GAAGCGTGAG AATTTTGAAC GTGGCAATCT GGAACTCGAG
    AAACGGAGAC AAGCGCTCTT GGAGCAGCAG CGCAAAGAGC AAGAGCGCCT GGCACAGCTT
    GAACGTGCAG AGCAAGAACG GAAAGAGCGG GAGCGCCAGG AGCAAGAGCG CAAAAGGCAA
    CTGGAGTTGG AGAAACAACT GGAAAAGCAA CGGGAGCTAG AACGTCAGAG AGAAGAGGAG
    AGGAGGAAAG AAATTGAGAG ACGAGAGGCT GCAAAGAGAG AACTTGAAAG ACAGCGGCAA
    CTGGAATGGG AACGAAATCG AAGACAAGAG CTACTCAATC AGAGAAACAA AGAACAAGAA
    GACATAGTTG TACTGAAAGC AAAGAAGAAG ACTCTAGAAT TTGAATTAGA AGCTCTTAAT
    GATAAAAAGC ATCAACTAGA AGGAAAACTT CAAGATATCA GGTGTCGATT GACCACCCAA
    CGGCAAGAAA TTGAGAGTAC AAATAAATCC AGAGAGTTGA GAATTGCTGA AATCACCCAT
    CTACAGCAAC AATTACAGGA AGCCCAGCAA ATGCTTGGAA GACTTATTCC AGAAAAGCAG
    ATACTCAATG ACCAATTAAA ACAAGTTCAA CAGAACAGTT TGCACAGAGA TTCACTGCTT
    ACACTCAAAA GAGCGTTAGA AGCAAAAGAA CTAGCTCGGC AGCAGCTACG CGATCAACTG
    GATGAAGTTG AGAAAGAAAC CAGATCAAAA CTACAGGAGA TTGATATTTT CAATAATCAG
    CTGAAGGAAC TAAGAGAAAT ACATAGTAAG CAACAACTCC AGAAACAAAA GTCCATAGAG
    GCCGAACGTC TGAAACAGAA AGAACAAGAG CGAAAAACCA TAGAATTAGA AAAACAAAAA
    GAAGAAGCCC AAAGACGAGC TCAGGAGAGG GACAAGCAGT GGATTGAGCA TGTGCAGCAG
    GACGATGAGC ATCAGCGGCC AAGGAAGATC CATGATGAAG AAAAACTCCG AAGGGAAGAG
    AGTGTCAAAA AGAAGGACAG TGAGGAAAAA AGCAAACTGG ACACGCAAGA CAAGATGAGT
    CGGCTTTTCC ACCAGCATCA AGAGCCAGCC AAGCCAGCTG TCCAGGCACC CTGGTCCACC
    ACAGAAAAAG GCCCACTTAC AATTTCTGCA CAGGAGAACG TAAAGGTGGT GTATTACCGA
    GCGCTCTATC CCTTCGAATC CAGAAGTCAT GATGAGATCA CGATCCAGCC TGGAGACATA
    GTAATGGTTA AAGGGGAATG GGTGGATGAA AGCCAAACTG GAGAACCGGG CTGGCTTGGA
    GGAGAATTAA AAGGAAAGAC GGGATGGTTC CCTGCTAACT ACGCAGAGAA AATCCCAGAA
    AACGAGGTGC CCGCTCCAGT CAAACCCGGG CCCGACCTCA CCTCTGCCCC TGCGCCCAAA
    CTGGCCGTGC GTGAGACCCC CGCTCCGGTG GCAGTGAGCG CTCCGGAGCC CTCCACGACC
    CCCAACAGCT GGGCTGACTT CAGCTCCACG TGGCCCACCA GCACGAATGA GAAGCCAGAA
    ACCGACAACT GGGACGCATG GGCAGCCCAG CCCTCACTCA CTGTTCCCAG TGCTGGCCAG
    CTAAGGCAGA GATCAGCGTT TACTCCAGCC ACGGCCACTG GCTCCTCTCC ATCTCCCGTT
    TTAGGCCAGG GTGAGAAGGT GGAAGGGCTA CAAGCACAAG CCCTGTATCC TTGGAGAGCC
    AAAAAAGACA ACCACTTAAA TTTTAACAAA AATGACGTCA TCACCGTCCT GGAACAGCAA
    GACATGTGGT GGTTTGGAGA AGTCCAAGGT CAGAAGGGTT GGTTCCCCAA GTCTTACGTG
    AAACTCATTT CAGGGCCCAT AAGGAAATCC ACAAGCATGG ATTCTGGTTC TTCAGAAAGT
    CCCGCTAGTC TAAAGAGAGT AGCTTCCCCA GCAGCCAAGC CGGCCATTTC GGGAGAAGAA
    TTTATTGCCA TGTACACTTA CGAGAGTTCT GAGCAAGGCG ATTTAACGTT TCAGCAAGGG
    GATGTGATTT TGGTTACCAA GAAAGATGGT GACTGGTGGA CAGGAGCAGT GGGCGACAAG
    TCCGGAGTCT TCCCTTCTAA CTATGTGAGG CTTAAAGATT CAGAGGGCTC TGGAACTGCT
    GGGAAAACAG GGAGTTTAGG AAAAAAACCT GAAATTGCGC AGGTTATCGC CTGCTACACA
    GCTACTGGTC CCGAGCAGCT CACCCTGGCC CCTGGTCAGC TGATTTTGAT CCGAAAAAAG
    AACCCAGGCG GTTGGTGGGA AGGAGAGCTG CAAGCCCGTG GGAAGAAGCG CCAGATAGGC
    TGGTTCCCCG CGAATTATGT AAAACTTTTG AGCCCTGGCA CGAGCAAAAT CACTCCAACG
    GAGCCACCTA AGCCCACGGC GTTCCCGGCA GTGTGCCAGG TGATCGGCGT GTACGACTAC
    AGCGCCCAGA ACGACGACGA GCTGGCCTTC AGCAAGGGCC AGATCATCAA CGTCCTCAAC
    AAAGACGACC CGGACTGGTG GAAGGGCGAG GTCAACGGGC AAGTGGGGCT CTTCCCCTCC
    AACTACGTGA AGCTGACCAC GGACATGGAC CCAAGCCAGC AATGGTGCTC AGACTTACAC
    CTCTTGGATA TGTTGACCCC AACGGAGAGA AAGCGACAAG GATACATCCA TGAGCTCATT
    GTCACAGAGG AGAACTATGT GAATGACCTG CAGCTGGTCA CAGAGATCTT TCAGAAACCC
    CTGATGGAGT CTGAGCTGCT GACAGAAAAA GAGGGTGCTA TGATTTTTGT TAACTGGAAG
    GAGCTGATTA TGTGTAATAT CAAACTATTG AAAGCGCTGA GGGTCCGCAA GAAGATGTCG
    GGGGAGAAGA TGCCCGTGAA GATGATCGGC GACATCCTGA CGGCGCAGCT GCCGCACATG
    CAGCCTTACA TCCGCTTCTG CAGCTGTCAG CTCAACGGGG CTGCCCTCAT CCAGCAGAAG
    ACCGACGAGG CCCCGGACTT CAAGGAGTTT GTCAAAAGGC TGGCAATGGA CCCTCGATGT
    AAAGGGATGC CACTCTCTAG TTTTATCCTG AAGCCTATGC AACGCGTAAC GAGATACCCA
    CTGATCATTA AAAATATCCT GGAAAACACT CCTGAGAACC ACCCCGACCA CAGCCACCTG
    AAGCACGCCC TGGAGAAGGC CGAAGAGCTG TGTTCACAGG TGAACGAAGG GGTGCGCGAG
    AAGGAGAACT CCGACCGCCT CGAGTGGATC CAGGCGCACG TGCAGTGCGA AGGCCTGTCC
    GAGCAACTTG TGTTCAATTC AGTGACCAAT TGCTTGGGGC CACGCAAGTT TCTGCACAGT
    GGGAAACTCT ACAAGGCCAA GAGCAACAAG GAGCTGTATG GCTTCCTCTT CAACGACTTC
    CTCCTGCTGA CCCAAATCAT AAAGCCCTTG GGCTCTTCCG GCACCGACAA AGTCTTCAGT
    CCCAAATCTA ACCTGCAGTA TAAAATGTAC AAAACACCTA TTTTCCTGAA TGAGGTTCTA
    GTAAAATTAC CCACTGACCC TTCTGGAGAT GAGCCCATCT TCCACATCTC CCACATTGAC
    CGAGTCTACA CCCTCCGAGC CGAAAGCATA AATGAAAGGA CTGCCTGGGT GCAGAAAATC
    AAAGCTGCTT CAGAACTTTA TATAGAGACT GAGAAGAAGA AGCGGGAGAA GGCGTACCTG
    GTCCGTTCCC AAAGGGCGAC AGGTATTGGA AGGTTGATGG TGAACGTCGT TGAGGGCATT
    GAGCTGAAAC CCTGTCGATC ACATGGAAAG AGTAACCCGT ACTGTGAGGT GACCATGGGT
    TCCCAGTGTC ACATCACCAA GACGATCCAG GACACTCTGA ATCCCAAGTG GAATTCCAAC
    TGCCAGTTCT TCATCAAAGA CCTGGAGCAG GAGGTCCTGT GCATCACCGT GTTTGAGAGG
    GACCAGTTCT CTCCAGACGA TTTTTTGGGT CGGACAGAGA TCCGTGTGGC CGACATCAAG
    AAGGACCAGG GTTCCAAGGG TCCAGTTACA AAGTGTCTCC TGCTACACGA AGTTCCCACA
    GGAGAGATTG TGGTCCGCTT GGACCTGCAG CTGTTTGATG AGCCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268943.1
    CDS103..5070
    Translation

    Target ORF information:

    RefSeq Version XM_022413235.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris intersectin 1 (ITSN1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413235.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAACAATG ATGGACGAAT GGATCAAGTG GAGTTTTCCA TAGCTATGAA ACTCATCAAA 
    CTCAAGCTGC AAGGATATCA ACTTCCTTCT GCACTTCCCC CCGTCATGAA ACAGCAACCA
    GTTGCTATTT CTAGCGCACC AGCCTTTGGT ATAGGAGGTA TTGCCAGCAT GCCACCACTT
    ACAGCTGTTG CTCCAGTGCC AATGGGATCC ATTCCAGTTG TCGGAATGTC TCCACCTCTA
    GTATCTTCTG TTCCTACAGC AGCAGTGCCT CCCCTGGCTA ACGGGGCTCC CCCTGTTATA
    CAACCTCTGC CTGCATTTGC TCATCCTGCA GCCACATTGC CAAAGAGCTC TTCCTTCAGT
    AGATCTGGTC CCGGGTCACA ATTAAACACT AAGTTACAGA AGGCACAGTC ATTTGATGTG
    GCTAGTGTCC CTCCCGTGGC AGAATGGGCT GTCCCTCAGT CATCCAGACT TAAATACAGA
    CAGTTATTCA ACAGTCATGA CAAAACTATG AGTGGACACT TAACAGGTCC ACAAGCAAGG
    ACTATTCTTA TGCAATCAAG TTTGCCACAG GCTCAGCTGG CTTCAATATG GAATCTTTCT
    GACATTGATC AAGATGGAAA ACTCACAGCA GAAGAATTTA TCTTGGCTAT GCACCTAATT
    GATGTAGCTA TGTCTGGCCA ACCACTGCCA CCTGTCCTGC CTCCAGAGTA CATTCCACCT
    TCCTTTAGAC GAGTTCGATC TGGCAGTGGT ATATCAGTCA TCAGCTCAAC AGCAGTAGAT
    CAGAGGTTAC CAGAGGAACC AGTTTTAGAA GATGAACAAC AACAACTAGA AAAGAAATTA
    CCTGTAACCT TTGAAGATAA GAAGCGTGAG AATTTTGAAC GTGGCAATCT GGAACTCGAG
    AAACGGAGAC AAGCGCTCTT GGAGCAGCAG CGCAAAGAGC AAGAGCGCCT GGCACAGCTT
    GAACGTGCAG AGCAAGAACG GAAAGAGCGG GAGCGCCAGG AGCAAGAGCG CAAAAGGCAA
    CTGGAGTTGG AGAAACAACT GGAAAAGCAA CGGGAGCTAG AACGTCAGAG AGAAGAGGAG
    AGGAGGAAAG AAATTGAGAG ACGAGAGGCT GCAAAGAGAG AACTTGAAAG ACAGCGGCAA
    CTGGAATGGG AACGAAATCG AAGACAAGAG CTACTCAATC AGAGAAACAA AGAACAAGAA
    GACATAGTTG TACTGAAAGC AAAGAAGAAG ACTCTAGAAT TTGAATTAGA AGCTCTTAAT
    GATAAAAAGC ATCAACTAGA AGGAAAACTT CAAGATATCA GGTGTCGATT GACCACCCAA
    CGGCAAGAAA TTGAGAGTAC AAATAAATCC AGAGAGTTGA GAATTGCTGA AATCACCCAT
    CTACAGCAAC AATTACAGGA AGCCCAGCAA ATGCTTGGAA GACTTATTCC AGAAAAGCAG
    ATACTCAATG ACCAATTAAA ACAAGTTCAA CAGAACAGTT TGCACAGAGA TTCACTGCTT
    ACACTCAAAA GAGCGTTAGA AGCAAAAGAA CTAGCTCGGC AGCAGCTACG CGATCAACTG
    GATGAAGTTG AGAAAGAAAC CAGATCAAAA CTACAGGAGA TTGATATTTT CAATAATCAG
    CTGAAGGAAC TAAGAGAAAT ACATAGTAAG CAACAACTCC AGAAACAAAA GTCCATAGAG
    GCCGAACGTC TGAAACAGAA AGAACAAGAG CGAAAAACCA TAGAATTAGA AAAACAAAAA
    GAAGAAGCCC AAAGACGAGC TCAGGAGAGG GACAAGCAGT GGATTGAGCA TGTGCAGCAG
    GACGATGAGC ATCAGCGGCC AAGGAAGATC CATGATGAAG AAAAACTCCG AAGGGAAGAG
    AGTGTCAAAA AGAAGGACAG TGAGGAAAAA AGCAAACTGG ACACGCAAGA CAAGATGAGT
    CGGCTTTTCC ACCAGCATCA AGAGCCAGCC AAGCCAGCTG TCCAGGCACC CTGGTCCACC
    ACAGAAAAAG GCCCACTTAC AATTTCTGCA CAGGAGAACG TAAAGGTGGT GTATTACCGA
    GCGCTCTATC CCTTCGAATC CAGAAGTCAT GATGAGATCA CGATCCAGCC TGGAGACATA
    GTAATGGTTA AAGGGGAATG GGTGGATGAA AGCCAAACTG GAGAACCGGG CTGGCTTGGA
    GGAGAATTAA AAGGAAAGAC GGGATGGTTC CCTGCTAACT ACGCAGAGAA AATCCCAGAA
    AACGAGGTGC CCGCTCCAGT CAAACCCGGG CCCGACCTCA CCTCTGCCCC TGCGCCCAAA
    CTGGCCGTGC GTGAGACCCC CGCTCCGGTG GCAGTGAGCG CTCCGGAGCC CTCCACGACC
    CCCAACAGCT GGGCTGACTT CAGCTCCACG TGGCCCACCA GCACGAATGA GAAGCCAGAA
    ACCGACAACT GGGACGCATG GGCAGCCCAG CCCTCACTCA CTGTTCCCAG TGCTGGCCAG
    CTAAGGCAGA GATCAGCGTT TACTCCAGCC ACGGCCACTG GCTCCTCTCC ATCTCCCGTT
    TTAGGCCAGG GTGAGAAGGT GGAAGGGCTA CAAGCACAAG CCCTGTATCC TTGGAGAGCC
    AAAAAAGACA ACCACTTAAA TTTTAACAAA AATGACGTCA TCACCGTCCT GGAACAGCAA
    GACATGTGGT GGTTTGGAGA AGTCCAAGGT CAGAAGGGTT GGTTCCCCAA GTCTTACGTG
    AAACTCATTT CAGGGCCCAT AAGGAAATCC ACAAGCATGG ATTCTGGTTC TTCAGAAAGT
    CCCGCTAGTC TAAAGAGAGT AGCTTCCCCA GCAGCCAAGC CGGCCATTTC GGGAGAAGAA
    TTTATTGCCA TGTACACTTA CGAGAGTTCT GAGCAAGGCG ATTTAACGTT TCAGCAAGGG
    GATGTGATTT TGGTTACCAA GAAAGATGGT GACTGGTGGA CAGGAGCAGT GGGCGACAAG
    TCCGGAGTCT TCCCTTCTAA CTATGTGAGG CTTAAAGATT CAGAGGGCTC TGGAACTGCT
    GGGAAAACAG GGAGTTTAGG AAAAAAACCT GAAATTGCGC AGGTTATCGC CTGCTACACA
    GCTACTGGTC CCGAGCAGCT CACCCTGGCC CCTGGTCAGC TGATTTTGAT CCGAAAAAAG
    AACCCAGGCG GTTGGTGGGA AGGAGAGCTG CAAGCCCGTG GGAAGAAGCG CCAGATAGGC
    TGGTTCCCCG CGAATTATGT AAAACTTTTG AGCCCTGGCA CGAGCAAAAT CACTCCAACG
    GAGCCACCTA AGCCCACGGC GTTCCCGGCA GTGTGCCAGG TGATCGGCGT GTACGACTAC
    AGCGCCCAGA ACGACGACGA GCTGGCCTTC AGCAAGGGCC AGATCATCAA CGTCCTCAAC
    AAAGACGACC CGGACTGGTG GAAGGGCGAG GTCAACGGGC AAGTGGGGCT CTTCCCCTCC
    AACTACGTGA AGCTGACCAC GGACATGGAC CCAAGCCAGC AATGGTGCTC AGACTTACAC
    CTCTTGGATA TGTTGACCCC AACGGAGAGA AAGCGACAAG GATACATCCA TGAGCTCATT
    GTCACAGAGG AGAACTATGT GAATGACCTG CAGCTGGTCA CAGAGATCTT TCAGAAACCC
    CTGATGGAGT CTGAGCTGCT GACAGAAAAA GAGGGTGCTA TGATTTTTGT TAACTGGAAG
    GAGCTGATTA TGTGTAATAT CAAACTATTG AAAGCGCTGA GGGTCCGCAA GAAGATGTCG
    GGGGAGAAGA TGCCCGTGAA GATGATCGGC GACATCCTGA CGGCGCAGCT GCCGCACATG
    CAGCCTTACA TCCGCTTCTG CAGCTGTCAG CTCAACGGGG CTGCCCTCAT CCAGCAGAAG
    ACCGACGAGG CCCCGGACTT CAAGGAGTTT GTCAAAAGGC TGGCAATGGA CCCTCGATGT
    AAAGGGATGC CACTCTCTAG TTTTATCCTG AAGCCTATGC AACGCGTAAC GAGATACCCA
    CTGATCATTA AAAATATCCT GGAAAACACT CCTGAGAACC ACCCCGACCA CAGCCACCTG
    AAGCACGCCC TGGAGAAGGC CGAAGAGCTG TGTTCACAGG TGAACGAAGG GGTGCGCGAG
    AAGGAGAACT CCGACCGCCT CGAGTGGATC CAGGCGCACG TGCAGTGCGA AGGCCTGTCC
    GAGCAACTTG TGTTCAATTC AGTGACCAAT TGCTTGGGGC CACGCAAGTT TCTGCACAGT
    GGGAAACTCT ACAAGGCCAA GAGCAACAAG GAGCTGTATG GCTTCCTCTT CAACGACTTC
    CTCCTGCTGA CCCAAATCAT AAAGCCCTTG GGCTCTTCCG GCACCGACAA AGTCTTCAGT
    CCCAAATCTA ACCTGCAGTA TAAAATGTAC AAAACACCTA TTTTCCTGAA TGAGGTTCTA
    GTAAAATTAC CCACTGACCC TTCTGGAGAT GAGCCCATCT TCCACATCTC CCACATTGAC
    CGAGTCTACA CCCTCCGAGC CGAAAGCATA AATGAAAGGA CTGCCTGGGT GCAGAAAATC
    AAAGCTGCTT CAGAACTTTA TATAGAGACT GAGAAGAAGA AGCGGGAGAA GGCGTACCTG
    GTCCGTTCCC AAAGGGCGAC AGGTATTGGA AGGTTGATGG TGAACGTCGT TGAGGGCATT
    GAGCTGAAAC CCTGTCGATC ACATGGAAAG AGTAACCCGT ACTGTGAGGT GACCATGGGT
    TCCCAGTGTC ACATCACCAA GACGATCCAG GACACTCTGA ATCCCAAGTG GAATTCCAAC
    TGCCAGTTCT TCATCAAAGA CCTGGAGCAG GAGGTCCTGT GCATCACCGT GTTTGAGAGG
    GACCAGTTCT CTCCAGACGA TTTTTTGGGT CGGACAGAGA TCCGTGTGGC CGACATCAAG
    AAGGACCAGG GTTCCAAGGG TCCAGTTACA AAGTGTCTCC TGCTACACGA AGTTCCCACA
    GGAGAGATTG TGGTCCGCTT GGACCTGCAG CTGTTTGATG AGCCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe283919
    Clone ID Related Accession (Same CDS sequence) XM_022413236.1
    Accession Version XM_022413236.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4938bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product intersectin-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCTCAGT TTCCAACACC TTTTGGAGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAA 
    GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG
    ATATGGGCGC TAGCTGACAT GAACAATGAT GGACGAATGG ATCAAGTGGA GTTTTCCATA
    GCTATGAAAC TCATCAAACT CAAGCTGCAA GGATATCAAC TTCCTTCTGC ACTTCCCCCC
    GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CCTTTGGTAT AGGAGGTATT
    GCCAGCATGC CACCACTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTC
    GGAATGTCTC CACCTCTAGT ATCTTCTGTT CCTACAGCAG CAGTGCCTCC CCTGGCTAAC
    GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA
    AAGAGCTCTT CCTTCAGTAG ATCTGGTCCC GGGTCACAAT TAAACACTAA GTTACAGAAG
    GCACAGTCAT TTGATGTGGC TAGTGTCCCT CCCGTGGCAG AATGGGCTGT CCCTCAGTCA
    TCCAGACTTA AATACAGACA GTTATTCAAC AGTCATGACA AAACTATGAG TGGACACTTA
    ACAGGTCCAC AAGCAAGGAC TATTCTTATG CAATCAAGTT TGCCACAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC
    TTGGCTATGC ACCTAATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT
    CCAGAGTACA TTCCACCTTC CTTTAGACGA GTTCGATCTG GCAGTGGTAT ATCAGTCATC
    AGCTCAACAG CAGTAGATCA GAGGTTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA
    CAACTAGAAA AGAAATTACC TGTAACCTTT GAAGATAAGA AGCGTGAGAA TTTTGAACGT
    GGCAATCTGG AACTCGAGAA ACGGAGACAA GCGCTCTTGG AGCAGCAGCG CAAAGAGCAA
    GAGCGCCTGG CACAGCTTGA ACGTGCAGAG CAAGAACGGA AAGAGCGGGA GCGCCAGGAG
    CAAGAGCGCA AAAGGCAACT GGAGTTGGAG AAACAACTGG AAAAGCAACG GGAGCTAGAA
    CGTCAGAGAG AAGAGGAGAG GAGGAAAGAA ATTGAGAGAC GAGAGGCTGC AAAGAGAGAA
    CTTGAAAGAC AGCGGCAACT GGAATGGGAA CGAAATCGAA GACAAGAGCT ACTCAATCAG
    AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAGAAGAC TCTAGAATTT
    GAATTAGAAG CTCTTAATGA TAAAAAGCAT CAACTAGAAG GAAAACTTCA AGATATCAGG
    TGTCGATTGA CCACCCAACG GCAAGAAATT GAGAGTACAA ATAAATCCAG AGAGTTGAGA
    ATTGCTGAAA TCACCCATCT ACAGCAACAA TTACAGGAAG CCCAGCAAAT GCTTGGAAGA
    CTTATTCCAG AAAAGCAGAT ACTCAATGAC CAATTAAAAC AAGTTCAACA GAACAGTTTG
    CACAGAGATT CACTGCTTAC ACTCAAAAGA GCGTTAGAAG CAAAAGAACT AGCTCGGCAG
    CAGCTACGCG ATCAACTGGA TGAAGTTGAG AAAGAAACCA GATCAAAACT ACAGGAGATT
    GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ATAGTAAGCA ACAACTCCAG
    AAACAAAAGT CCATAGAGGC CGAACGTCTG AAACAGAAAG AACAAGAGCG AAAAACCATA
    GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAGAGGGA CAAGCAGTGG
    ATTGAGCATG TGCAGCAGGA CGATGAGCAT CAGCGGCCAA GGAAGATCCA TGATGAAGAA
    AAACTCCGAA GGGAAGAGAG TGTCAAAAAG AAGGACAGTG AGGAAAAAAG CAAACTGGAC
    ACGCAAGACA AGATGAGTCG GCTTTTCCAC CAGCATCAAG AGCCAGCCAA GCCAGCTGTC
    CAGGCACCCT GGTCCACCAC AGAAAAAGGC CCACTTACAA TTTCTGCACA GGAGAACGTA
    AAGGTGGTGT ATTACCGAGC GCTCTATCCC TTCGAATCCA GAAGTCATGA TGAGATCACG
    ATCCAGCCTG GAGACATAGT AATGGTGGAT GAAAGCCAAA CTGGAGAACC GGGCTGGCTT
    GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCTA ACTACGCAGA GAAAATCCCA
    GAAAACGAGG TGCCCGCTCC AGTCAAACCC GGGCCCGACC TCACCTCTGC CCCTGCGCCC
    AAACTGGCCG TGCGTGAGAC CCCCGCTCCG GTGGCAGTGA GCGCTCCGGA GCCCTCCACG
    ACCCCCAACA GCTGGGCTGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAGCCA
    GAAACCGACA ACTGGGACGC ATGGGCAGCC CAGCCCTCAC TCACTGTTCC CAGTGCTGGC
    CAGCTAAGGC AGAGATCAGC GTTTACTCCA GCCACGGCCA CTGGCTCCTC TCCATCTCCC
    GTTTTAGGCC AGGGTGAGAA GGTGGAAGGG CTACAAGCAC AAGCCCTGTA TCCTTGGAGA
    GCCAAAAAAG ACAACCACTT AAATTTTAAC AAAAATGACG TCATCACCGT CCTGGAACAG
    CAAGACATGT GGTGGTTTGG AGAAGTCCAA GGTCAGAAGG GTTGGTTCCC CAAGTCTTAC
    GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCCACAAGCA TGGATTCTGG TTCTTCAGAA
    AGTCCCGCTA GTCTAAAGAG AGTAGCTTCC CCAGCAGCCA AGCCGGCCAT TTCGGGAGAA
    GAAATTGCGC AGGTTATCGC CTGCTACACA GCTACTGGTC CCGAGCAGCT CACCCTGGCC
    CCTGGTCAGC TGATTTTGAT CCGAAAAAAG AACCCAGGCG GTTGGTGGGA AGGAGAGCTG
    CAAGCCCGTG GGAAGAAGCG CCAGATAGGC TGGTTCCCCG CGAATTATGT AAAACTTTTG
    AGCCCTGGCA CGAGCAAAAT CACTCCAACG GAGCCACCTA AGCCCACGGC GTTCCCGGCA
    GTGTGCCAGG TGATCGGCGT GTACGACTAC AGCGCCCAGA ACGACGACGA GCTGGCCTTC
    AGCAAGGGCC AGATCATCAA CGTCCTCAAC AAAGACGACC CGGACTGGTG GAAGGGCGAG
    GTCAACGGGC AAGTGGGGCT CTTCCCCTCC AACTACGTGA AGCTGACCAC GGACATGGAC
    CCAAGCCAGC AATGGTGCTC AGACTTACAC CTCTTGGATA TGTTGACCCC AACGGAGAGA
    AAGCGACAAG GATACATCCA TGAGCTCATT GTCACAGAGG AGAACTATGT GAATGACCTG
    CAGCTGGTCA CAGAGATCTT TCAGAAACCC CTGATGGAGT CTGAGCTGCT GACAGAAAAA
    GAGGGTGCTA TGATTTTTGT TAACTGGAAG GAGCTGATTA TGTGTAATAT CAAACTATTG
    AAAGCGCTGA GGGTCCGCAA GAAGATGTCG GGGGAGAAGA TGCCCGTGAA GATGATCGGC
    GACATCCTGA CGGCGCAGCT GCCGCACATG CAGCCTTACA TCCGCTTCTG CAGCTGTCAG
    CTCAACGGGG CTGCCCTCAT CCAGCAGAAG ACCGACGAGG CCCCGGACTT CAAGGAGTTT
    GTCAAAAGGC TGGCAATGGA CCCTCGATGT AAAGGGATGC CACTCTCTAG TTTTATCCTG
    AAGCCTATGC AACGCGTAAC GAGATACCCA CTGATCATTA AAAATATCCT GGAAAACACT
    CCTGAGAACC ACCCCGACCA CAGCCACCTG AAGCACGCCC TGGAGAAGGC CGAAGAGCTG
    TGTTCACAGG TGAACGAAGG GGTGCGCGAG AAGGAGAACT CCGACCGCCT CGAGTGGATC
    CAGGCGCACG TGCAGTGCGA AGGCCTGTCC GAGCAACTTG TGTTCAATTC AGTGACCAAT
    TGCTTGGGGC CACGCAAGTT TCTGCACAGT GGGAAACTCT ACAAGGCCAA GAGCAACAAG
    GAGCTGTATG GCTTCCTCTT CAACGACTTC CTCCTGCTGA CCCAAATCAT AAAGCCCTTG
    GGCTCTTCCG GCACCGACAA AGTCTTCAGT CCCAAATCTA ACCTGCAGTA TAAAATGTAC
    AAAACACCTA TTTTCCTGAA TGAGGTTCTA GTAAAATTAC CCACTGACCC TTCTGGAGAT
    GAGCCCATCT TCCACATCTC CCACATTGAC CGAGTCTACA CCCTCCGAGC CGAAAGCATA
    AATGAAAGGA CTGCCTGGGT GCAGAAAATC AAAGCTGCTT CAGAACTTTA TATAGAGACT
    GAGAAGAAGA AGCGGGAGAA GGCGTACCTG GTCCGTTCCC AAAGGGCGAC AGGTATTGGA
    AGGTTGATGG TGAACGTCGT TGAGGGCATT GAGCTGAAAC CCTGTCGATC ACATGGAAAG
    AGTAACCCGT ACTGTGAGGT GACCATGGGT TCCCAGTGTC ACATCACCAA GACGATCCAG
    GACACTCTGA ATCCCAAGTG GAATTCCAAC TGCCAGTTCT TCATCAAAGA CCTGGAGCAG
    GAGGTCCTGT GCATCACCGT GTTTGAGAGG GACCAGTTCT CTCCAGACGA TTTTTTGGGT
    CGGACAGAGA TCCGTGTGGC CGACATCAAG AAGGACCAGG GTTCCAAGGG TCCAGTTACA
    AAGTGTCTCC TGCTACACGA AGTTCCCACA GGAGAGATTG TGGTCCGCTT GGACCTGCAG
    CTGTTTGATG AGCCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268944.1
    CDS258..5195
    Translation

    Target ORF information:

    RefSeq Version XM_022413236.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris intersectin 1 (ITSN1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413236.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCTCAGT TTCCAACACC TTTTGGAGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAA 
    GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG
    ATATGGGCGC TAGCTGACAT GAACAATGAT GGACGAATGG ATCAAGTGGA GTTTTCCATA
    GCTATGAAAC TCATCAAACT CAAGCTGCAA GGATATCAAC TTCCTTCTGC ACTTCCCCCC
    GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CCTTTGGTAT AGGAGGTATT
    GCCAGCATGC CACCACTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTC
    GGAATGTCTC CACCTCTAGT ATCTTCTGTT CCTACAGCAG CAGTGCCTCC CCTGGCTAAC
    GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA
    AAGAGCTCTT CCTTCAGTAG ATCTGGTCCC GGGTCACAAT TAAACACTAA GTTACAGAAG
    GCACAGTCAT TTGATGTGGC TAGTGTCCCT CCCGTGGCAG AATGGGCTGT CCCTCAGTCA
    TCCAGACTTA AATACAGACA GTTATTCAAC AGTCATGACA AAACTATGAG TGGACACTTA
    ACAGGTCCAC AAGCAAGGAC TATTCTTATG CAATCAAGTT TGCCACAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC
    TTGGCTATGC ACCTAATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT
    CCAGAGTACA TTCCACCTTC CTTTAGACGA GTTCGATCTG GCAGTGGTAT ATCAGTCATC
    AGCTCAACAG CAGTAGATCA GAGGTTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA
    CAACTAGAAA AGAAATTACC TGTAACCTTT GAAGATAAGA AGCGTGAGAA TTTTGAACGT
    GGCAATCTGG AACTCGAGAA ACGGAGACAA GCGCTCTTGG AGCAGCAGCG CAAAGAGCAA
    GAGCGCCTGG CACAGCTTGA ACGTGCAGAG CAAGAACGGA AAGAGCGGGA GCGCCAGGAG
    CAAGAGCGCA AAAGGCAACT GGAGTTGGAG AAACAACTGG AAAAGCAACG GGAGCTAGAA
    CGTCAGAGAG AAGAGGAGAG GAGGAAAGAA ATTGAGAGAC GAGAGGCTGC AAAGAGAGAA
    CTTGAAAGAC AGCGGCAACT GGAATGGGAA CGAAATCGAA GACAAGAGCT ACTCAATCAG
    AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAGAAGAC TCTAGAATTT
    GAATTAGAAG CTCTTAATGA TAAAAAGCAT CAACTAGAAG GAAAACTTCA AGATATCAGG
    TGTCGATTGA CCACCCAACG GCAAGAAATT GAGAGTACAA ATAAATCCAG AGAGTTGAGA
    ATTGCTGAAA TCACCCATCT ACAGCAACAA TTACAGGAAG CCCAGCAAAT GCTTGGAAGA
    CTTATTCCAG AAAAGCAGAT ACTCAATGAC CAATTAAAAC AAGTTCAACA GAACAGTTTG
    CACAGAGATT CACTGCTTAC ACTCAAAAGA GCGTTAGAAG CAAAAGAACT AGCTCGGCAG
    CAGCTACGCG ATCAACTGGA TGAAGTTGAG AAAGAAACCA GATCAAAACT ACAGGAGATT
    GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ATAGTAAGCA ACAACTCCAG
    AAACAAAAGT CCATAGAGGC CGAACGTCTG AAACAGAAAG AACAAGAGCG AAAAACCATA
    GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAGAGGGA CAAGCAGTGG
    ATTGAGCATG TGCAGCAGGA CGATGAGCAT CAGCGGCCAA GGAAGATCCA TGATGAAGAA
    AAACTCCGAA GGGAAGAGAG TGTCAAAAAG AAGGACAGTG AGGAAAAAAG CAAACTGGAC
    ACGCAAGACA AGATGAGTCG GCTTTTCCAC CAGCATCAAG AGCCAGCCAA GCCAGCTGTC
    CAGGCACCCT GGTCCACCAC AGAAAAAGGC CCACTTACAA TTTCTGCACA GGAGAACGTA
    AAGGTGGTGT ATTACCGAGC GCTCTATCCC TTCGAATCCA GAAGTCATGA TGAGATCACG
    ATCCAGCCTG GAGACATAGT AATGGTGGAT GAAAGCCAAA CTGGAGAACC GGGCTGGCTT
    GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCTA ACTACGCAGA GAAAATCCCA
    GAAAACGAGG TGCCCGCTCC AGTCAAACCC GGGCCCGACC TCACCTCTGC CCCTGCGCCC
    AAACTGGCCG TGCGTGAGAC CCCCGCTCCG GTGGCAGTGA GCGCTCCGGA GCCCTCCACG
    ACCCCCAACA GCTGGGCTGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAGCCA
    GAAACCGACA ACTGGGACGC ATGGGCAGCC CAGCCCTCAC TCACTGTTCC CAGTGCTGGC
    CAGCTAAGGC AGAGATCAGC GTTTACTCCA GCCACGGCCA CTGGCTCCTC TCCATCTCCC
    GTTTTAGGCC AGGGTGAGAA GGTGGAAGGG CTACAAGCAC AAGCCCTGTA TCCTTGGAGA
    GCCAAAAAAG ACAACCACTT AAATTTTAAC AAAAATGACG TCATCACCGT CCTGGAACAG
    CAAGACATGT GGTGGTTTGG AGAAGTCCAA GGTCAGAAGG GTTGGTTCCC CAAGTCTTAC
    GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCCACAAGCA TGGATTCTGG TTCTTCAGAA
    AGTCCCGCTA GTCTAAAGAG AGTAGCTTCC CCAGCAGCCA AGCCGGCCAT TTCGGGAGAA
    GAAATTGCGC AGGTTATCGC CTGCTACACA GCTACTGGTC CCGAGCAGCT CACCCTGGCC
    CCTGGTCAGC TGATTTTGAT CCGAAAAAAG AACCCAGGCG GTTGGTGGGA AGGAGAGCTG
    CAAGCCCGTG GGAAGAAGCG CCAGATAGGC TGGTTCCCCG CGAATTATGT AAAACTTTTG
    AGCCCTGGCA CGAGCAAAAT CACTCCAACG GAGCCACCTA AGCCCACGGC GTTCCCGGCA
    GTGTGCCAGG TGATCGGCGT GTACGACTAC AGCGCCCAGA ACGACGACGA GCTGGCCTTC
    AGCAAGGGCC AGATCATCAA CGTCCTCAAC AAAGACGACC CGGACTGGTG GAAGGGCGAG
    GTCAACGGGC AAGTGGGGCT CTTCCCCTCC AACTACGTGA AGCTGACCAC GGACATGGAC
    CCAAGCCAGC AATGGTGCTC AGACTTACAC CTCTTGGATA TGTTGACCCC AACGGAGAGA
    AAGCGACAAG GATACATCCA TGAGCTCATT GTCACAGAGG AGAACTATGT GAATGACCTG
    CAGCTGGTCA CAGAGATCTT TCAGAAACCC CTGATGGAGT CTGAGCTGCT GACAGAAAAA
    GAGGGTGCTA TGATTTTTGT TAACTGGAAG GAGCTGATTA TGTGTAATAT CAAACTATTG
    AAAGCGCTGA GGGTCCGCAA GAAGATGTCG GGGGAGAAGA TGCCCGTGAA GATGATCGGC
    GACATCCTGA CGGCGCAGCT GCCGCACATG CAGCCTTACA TCCGCTTCTG CAGCTGTCAG
    CTCAACGGGG CTGCCCTCAT CCAGCAGAAG ACCGACGAGG CCCCGGACTT CAAGGAGTTT
    GTCAAAAGGC TGGCAATGGA CCCTCGATGT AAAGGGATGC CACTCTCTAG TTTTATCCTG
    AAGCCTATGC AACGCGTAAC GAGATACCCA CTGATCATTA AAAATATCCT GGAAAACACT
    CCTGAGAACC ACCCCGACCA CAGCCACCTG AAGCACGCCC TGGAGAAGGC CGAAGAGCTG
    TGTTCACAGG TGAACGAAGG GGTGCGCGAG AAGGAGAACT CCGACCGCCT CGAGTGGATC
    CAGGCGCACG TGCAGTGCGA AGGCCTGTCC GAGCAACTTG TGTTCAATTC AGTGACCAAT
    TGCTTGGGGC CACGCAAGTT TCTGCACAGT GGGAAACTCT ACAAGGCCAA GAGCAACAAG
    GAGCTGTATG GCTTCCTCTT CAACGACTTC CTCCTGCTGA CCCAAATCAT AAAGCCCTTG
    GGCTCTTCCG GCACCGACAA AGTCTTCAGT CCCAAATCTA ACCTGCAGTA TAAAATGTAC
    AAAACACCTA TTTTCCTGAA TGAGGTTCTA GTAAAATTAC CCACTGACCC TTCTGGAGAT
    GAGCCCATCT TCCACATCTC CCACATTGAC CGAGTCTACA CCCTCCGAGC CGAAAGCATA
    AATGAAAGGA CTGCCTGGGT GCAGAAAATC AAAGCTGCTT CAGAACTTTA TATAGAGACT
    GAGAAGAAGA AGCGGGAGAA GGCGTACCTG GTCCGTTCCC AAAGGGCGAC AGGTATTGGA
    AGGTTGATGG TGAACGTCGT TGAGGGCATT GAGCTGAAAC CCTGTCGATC ACATGGAAAG
    AGTAACCCGT ACTGTGAGGT GACCATGGGT TCCCAGTGTC ACATCACCAA GACGATCCAG
    GACACTCTGA ATCCCAAGTG GAATTCCAAC TGCCAGTTCT TCATCAAAGA CCTGGAGCAG
    GAGGTCCTGT GCATCACCGT GTTTGAGAGG GACCAGTTCT CTCCAGACGA TTTTTTGGGT
    CGGACAGAGA TCCGTGTGGC CGACATCAAG AAGGACCAGG GTTCCAAGGG TCCAGTTACA
    AAGTGTCTCC TGCTACACGA AGTTCCCACA GGAGAGATTG TGGTCCGCTT GGACCTGCAG
    CTGTTTGATG AGCCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe283920
    Clone ID Related Accession (Same CDS sequence) XM_022413237.1
    Accession Version XM_022413237.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4587bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product intersectin-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGAGCGT TGTTGCAGAA GTACTCACAG CAGAAACAAT ATTGTGTCCC TCCCGTGGCA 
    GAATGGGCTG TCCCTCAGTC ATCCAGACTT AAATACAGAC AGTTATTCAA CAGTCATGAC
    AAAACTATGA GTGGACACTT AACAGGTCCA CAAGCAAGGA CTATTCTTAT GCAATCAAGT
    TTGCCACAGG CTCAGCTGGC TTCAATATGG AATCTTTCTG ACATTGATCA AGATGGAAAA
    CTCACAGCAG AAGAATTTAT CTTGGCTATG CACCTAATTG ATGTAGCTAT GTCTGGCCAA
    CCACTGCCAC CTGTCCTGCC TCCAGAGTAC ATTCCACCTT CCTTTAGACG AGTTCGATCT
    GGCAGTGGTA TATCAGTCAT CAGCTCAACA GCAGTAGATC AGAGGTTACC AGAGGAACCA
    GTTTTAGAAG ATGAACAACA ACAACTAGAA AAGAAATTAC CTGTAACCTT TGAAGATAAG
    AAGCGTGAGA ATTTTGAACG TGGCAATCTG GAACTCGAGA AACGGAGACA AGCGCTCTTG
    GAGCAGCAGC GCAAAGAGCA AGAGCGCCTG GCACAGCTTG AACGTGCAGA GCAAGAACGG
    AAAGAGCGGG AGCGCCAGGA GCAAGAGCGC AAAAGGCAAC TGGAGTTGGA GAAACAACTG
    GAAAAGCAAC GGGAGCTAGA ACGTCAGAGA GAAGAGGAGA GGAGGAAAGA AATTGAGAGA
    CGAGAGGCTG CAAAGAGAGA ACTTGAAAGA CAGCGGCAAC TGGAATGGGA ACGAAATCGA
    AGACAAGAGC TACTCAATCA GAGAAACAAA GAACAAGAAG ACATAGTTGT ACTGAAAGCA
    AAGAAGAAGA CTCTAGAATT TGAATTAGAA GCTCTTAATG ATAAAAAGCA TCAACTAGAA
    GGAAAACTTC AAGATATCAG GTGTCGATTG ACCACCCAAC GGCAAGAAAT TGAGAGTACA
    AATAAATCCA GAGAGTTGAG AATTGCTGAA ATCACCCATC TACAGCAACA ATTACAGGAA
    GCCCAGCAAA TGCTTGGAAG ACTTATTCCA GAAAAGCAGA TACTCAATGA CCAATTAAAA
    CAAGTTCAAC AGAACAGTTT GCACAGAGAT TCACTGCTTA CACTCAAAAG AGCGTTAGAA
    GCAAAAGAAC TAGCTCGGCA GCAGCTACGC GATCAACTGG ATGAAGTTGA GAAAGAAACC
    AGATCAAAAC TACAGGAGAT TGATATTTTC AATAATCAGC TGAAGGAACT AAGAGAAATA
    CATAGTAAGC AACAACTCCA GAAACAAAAG TCCATAGAGG CCGAACGTCT GAAACAGAAA
    GAACAAGAGC GAAAAACCAT AGAATTAGAA AAACAAAAAG AAGAAGCCCA AAGACGAGCT
    CAGGAGAGGG ACAAGCAGTG GATTGAGCAT GTGCAGCAGG ACGATGAGCA TCAGCGGCCA
    AGGAAGATCC ATGATGAAGA AAAACTCCGA AGGGAAGAGA GTGTCAAAAA GAAGGACAGT
    GAGGAAAAAA GCAAACTGGA CACGCAAGAC AAGATGAGTC GGCTTTTCCA CCAGCATCAA
    GAGCCAGCCA AGCCAGCTGT CCAGGCACCC TGGTCCACCA CAGAAAAAGG CCCACTTACA
    ATTTCTGCAC AGGAGAACGT AAAGGTGGTG TATTACCGAG CGCTCTATCC CTTCGAATCC
    AGAAGTCATG ATGAGATCAC GATCCAGCCT GGAGACATAG TAATGGTTAA AGGGGAATGG
    GTGGATGAAA GCCAAACTGG AGAACCGGGC TGGCTTGGAG GAGAATTAAA AGGAAAGACG
    GGATGGTTCC CTGCTAACTA CGCAGAGAAA ATCCCAGAAA ACGAGGTGCC CGCTCCAGTC
    AAACCCGGGC CCGACCTCAC CTCTGCCCCT GCGCCCAAAC TGGCCGTGCG TGAGACCCCC
    GCTCCGGTGG CAGTGAGCGC TCCGGAGCCC TCCACGACCC CCAACAGCTG GGCTGACTTC
    AGCTCCACGT GGCCCACCAG CACGAATGAG AAGCCAGAAA CCGACAACTG GGACGCATGG
    GCAGCCCAGC CCTCACTCAC TGTTCCCAGT GCTGGCCAGC TAAGGCAGAG ATCAGCGTTT
    ACTCCAGCCA CGGCCACTGG CTCCTCTCCA TCTCCCGTTT TAGGCCAGGG TGAGAAGGTG
    GAAGGGCTAC AAGCACAAGC CCTGTATCCT TGGAGAGCCA AAAAAGACAA CCACTTAAAT
    TTTAACAAAA ATGACGTCAT CACCGTCCTG GAACAGCAAG ACATGTGGTG GTTTGGAGAA
    GTCCAAGGTC AGAAGGGTTG GTTCCCCAAG TCTTACGTGA AACTCATTTC AGGGCCCATA
    AGGAAATCCA CAAGCATGGA TTCTGGTTCT TCAGAAAGTC CCGCTAGTCT AAAGAGAGTA
    GCTTCCCCAG CAGCCAAGCC GGCCATTTCG GGAGAAGAAT TTATTGCCAT GTACACTTAC
    GAGAGTTCTG AGCAAGGCGA TTTAACGTTT CAGCAAGGGG ATGTGATTTT GGTTACCAAG
    AAAGATGGTG ACTGGTGGAC AGGAGCAGTG GGCGACAAGT CCGGAGTCTT CCCTTCTAAC
    TATGTGAGGC TTAAAGATTC AGAGGGCTCT GGAACTGCTG GGAAAACAGG GAGTTTAGGA
    AAAAAACCTG AAATTGCGCA GGTTATCGCC TGCTACACAG CTACTGGTCC CGAGCAGCTC
    ACCCTGGCCC CTGGTCAGCT GATTTTGATC CGAAAAAAGA ACCCAGGCGG TTGGTGGGAA
    GGAGAGCTGC AAGCCCGTGG GAAGAAGCGC CAGATAGGCT GGTTCCCCGC GAATTATGTA
    AAACTTTTGA GCCCTGGCAC GAGCAAAATC ACTCCAACGG AGCCACCTAA GCCCACGGCG
    TTCCCGGCAG TGTGCCAGGT GATCGGCGTG TACGACTACA GCGCCCAGAA CGACGACGAG
    CTGGCCTTCA GCAAGGGCCA GATCATCAAC GTCCTCAACA AAGACGACCC GGACTGGTGG
    AAGGGCGAGG TCAACGGGCA AGTGGGGCTC TTCCCCTCCA ACTACGTGAA GCTGACCACG
    GACATGGACC CAAGCCAGCA ATGGTGCTCA GACTTACACC TCTTGGATAT GTTGACCCCA
    ACGGAGAGAA AGCGACAAGG ATACATCCAT GAGCTCATTG TCACAGAGGA GAACTATGTG
    AATGACCTGC AGCTGGTCAC AGAGATCTTT CAGAAACCCC TGATGGAGTC TGAGCTGCTG
    ACAGAAAAAG AGGGTGCTAT GATTTTTGTT AACTGGAAGG AGCTGATTAT GTGTAATATC
    AAACTATTGA AAGCGCTGAG GGTCCGCAAG AAGATGTCGG GGGAGAAGAT GCCCGTGAAG
    ATGATCGGCG ACATCCTGAC GGCGCAGCTG CCGCACATGC AGCCTTACAT CCGCTTCTGC
    AGCTGTCAGC TCAACGGGGC TGCCCTCATC CAGCAGAAGA CCGACGAGGC CCCGGACTTC
    AAGGAGTTTG TCAAAAGGCT GGCAATGGAC CCTCGATGTA AAGGGATGCC ACTCTCTAGT
    TTTATCCTGA AGCCTATGCA ACGCGTAACG AGATACCCAC TGATCATTAA AAATATCCTG
    GAAAACACTC CTGAGAACCA CCCCGACCAC AGCCACCTGA AGCACGCCCT GGAGAAGGCC
    GAAGAGCTGT GTTCACAGGT GAACGAAGGG GTGCGCGAGA AGGAGAACTC CGACCGCCTC
    GAGTGGATCC AGGCGCACGT GCAGTGCGAA GGCCTGTCCG AGCAACTTGT GTTCAATTCA
    GTGACCAATT GCTTGGGGCC ACGCAAGTTT CTGCACAGTG GGAAACTCTA CAAGGCCAAG
    AGCAACAAGG AGCTGTATGG CTTCCTCTTC AACGACTTCC TCCTGCTGAC CCAAATCATA
    AAGCCCTTGG GCTCTTCCGG CACCGACAAA GTCTTCAGTC CCAAATCTAA CCTGCAGTAT
    AAAATGTACA AAACACCTAT TTTCCTGAAT GAGGTTCTAG TAAAATTACC CACTGACCCT
    TCTGGAGATG AGCCCATCTT CCACATCTCC CACATTGACC GAGTCTACAC CCTCCGAGCC
    GAAAGCATAA ATGAAAGGAC TGCCTGGGTG CAGAAAATCA AAGCTGCTTC AGAACTTTAT
    ATAGAGACTG AGAAGAAGAA GCGGGAGAAG GCGTACCTGG TCCGTTCCCA AAGGGCGACA
    GGTATTGGAA GGTTGATGGT GAACGTCGTT GAGGGCATTG AGCTGAAACC CTGTCGATCA
    CATGGAAAGA GTAACCCGTA CTGTGAGGTG ACCATGGGTT CCCAGTGTCA CATCACCAAG
    ACGATCCAGG ACACTCTGAA TCCCAAGTGG AATTCCAACT GCCAGTTCTT CATCAAAGAC
    CTGGAGCAGG AGGTCCTGTG CATCACCGTG TTTGAGAGGG ACCAGTTCTC TCCAGACGAT
    TTTTTGGGTC GGACAGAGAT CCGTGTGGCC GACATCAAGA AGGACCAGGG TTCCAAGGGT
    CCAGTTACAA AGTGTCTCCT GCTACACGAA GTTCCCACAG GAGAGATTGT GGTCCGCTTG
    GACCTGCAGC TGTTTGATGA GCCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268945.1
    CDS61..4647
    Translation

    Target ORF information:

    RefSeq Version XM_022413237.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris intersectin 1 (ITSN1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413237.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGAGCGT TGTTGCAGAA GTACTCACAG CAGAAACAAT ATTGTGTCCC TCCCGTGGCA 
    GAATGGGCTG TCCCTCAGTC ATCCAGACTT AAATACAGAC AGTTATTCAA CAGTCATGAC
    AAAACTATGA GTGGACACTT AACAGGTCCA CAAGCAAGGA CTATTCTTAT GCAATCAAGT
    TTGCCACAGG CTCAGCTGGC TTCAATATGG AATCTTTCTG ACATTGATCA AGATGGAAAA
    CTCACAGCAG AAGAATTTAT CTTGGCTATG CACCTAATTG ATGTAGCTAT GTCTGGCCAA
    CCACTGCCAC CTGTCCTGCC TCCAGAGTAC ATTCCACCTT CCTTTAGACG AGTTCGATCT
    GGCAGTGGTA TATCAGTCAT CAGCTCAACA GCAGTAGATC AGAGGTTACC AGAGGAACCA
    GTTTTAGAAG ATGAACAACA ACAACTAGAA AAGAAATTAC CTGTAACCTT TGAAGATAAG
    AAGCGTGAGA ATTTTGAACG TGGCAATCTG GAACTCGAGA AACGGAGACA AGCGCTCTTG
    GAGCAGCAGC GCAAAGAGCA AGAGCGCCTG GCACAGCTTG AACGTGCAGA GCAAGAACGG
    AAAGAGCGGG AGCGCCAGGA GCAAGAGCGC AAAAGGCAAC TGGAGTTGGA GAAACAACTG
    GAAAAGCAAC GGGAGCTAGA ACGTCAGAGA GAAGAGGAGA GGAGGAAAGA AATTGAGAGA
    CGAGAGGCTG CAAAGAGAGA ACTTGAAAGA CAGCGGCAAC TGGAATGGGA ACGAAATCGA
    AGACAAGAGC TACTCAATCA GAGAAACAAA GAACAAGAAG ACATAGTTGT ACTGAAAGCA
    AAGAAGAAGA CTCTAGAATT TGAATTAGAA GCTCTTAATG ATAAAAAGCA TCAACTAGAA
    GGAAAACTTC AAGATATCAG GTGTCGATTG ACCACCCAAC GGCAAGAAAT TGAGAGTACA
    AATAAATCCA GAGAGTTGAG AATTGCTGAA ATCACCCATC TACAGCAACA ATTACAGGAA
    GCCCAGCAAA TGCTTGGAAG ACTTATTCCA GAAAAGCAGA TACTCAATGA CCAATTAAAA
    CAAGTTCAAC AGAACAGTTT GCACAGAGAT TCACTGCTTA CACTCAAAAG AGCGTTAGAA
    GCAAAAGAAC TAGCTCGGCA GCAGCTACGC GATCAACTGG ATGAAGTTGA GAAAGAAACC
    AGATCAAAAC TACAGGAGAT TGATATTTTC AATAATCAGC TGAAGGAACT AAGAGAAATA
    CATAGTAAGC AACAACTCCA GAAACAAAAG TCCATAGAGG CCGAACGTCT GAAACAGAAA
    GAACAAGAGC GAAAAACCAT AGAATTAGAA AAACAAAAAG AAGAAGCCCA AAGACGAGCT
    CAGGAGAGGG ACAAGCAGTG GATTGAGCAT GTGCAGCAGG ACGATGAGCA TCAGCGGCCA
    AGGAAGATCC ATGATGAAGA AAAACTCCGA AGGGAAGAGA GTGTCAAAAA GAAGGACAGT
    GAGGAAAAAA GCAAACTGGA CACGCAAGAC AAGATGAGTC GGCTTTTCCA CCAGCATCAA
    GAGCCAGCCA AGCCAGCTGT CCAGGCACCC TGGTCCACCA CAGAAAAAGG CCCACTTACA
    ATTTCTGCAC AGGAGAACGT AAAGGTGGTG TATTACCGAG CGCTCTATCC CTTCGAATCC
    AGAAGTCATG ATGAGATCAC GATCCAGCCT GGAGACATAG TAATGGTTAA AGGGGAATGG
    GTGGATGAAA GCCAAACTGG AGAACCGGGC TGGCTTGGAG GAGAATTAAA AGGAAAGACG
    GGATGGTTCC CTGCTAACTA CGCAGAGAAA ATCCCAGAAA ACGAGGTGCC CGCTCCAGTC
    AAACCCGGGC CCGACCTCAC CTCTGCCCCT GCGCCCAAAC TGGCCGTGCG TGAGACCCCC
    GCTCCGGTGG CAGTGAGCGC TCCGGAGCCC TCCACGACCC CCAACAGCTG GGCTGACTTC
    AGCTCCACGT GGCCCACCAG CACGAATGAG AAGCCAGAAA CCGACAACTG GGACGCATGG
    GCAGCCCAGC CCTCACTCAC TGTTCCCAGT GCTGGCCAGC TAAGGCAGAG ATCAGCGTTT
    ACTCCAGCCA CGGCCACTGG CTCCTCTCCA TCTCCCGTTT TAGGCCAGGG TGAGAAGGTG
    GAAGGGCTAC AAGCACAAGC CCTGTATCCT TGGAGAGCCA AAAAAGACAA CCACTTAAAT
    TTTAACAAAA ATGACGTCAT CACCGTCCTG GAACAGCAAG ACATGTGGTG GTTTGGAGAA
    GTCCAAGGTC AGAAGGGTTG GTTCCCCAAG TCTTACGTGA AACTCATTTC AGGGCCCATA
    AGGAAATCCA CAAGCATGGA TTCTGGTTCT TCAGAAAGTC CCGCTAGTCT AAAGAGAGTA
    GCTTCCCCAG CAGCCAAGCC GGCCATTTCG GGAGAAGAAT TTATTGCCAT GTACACTTAC
    GAGAGTTCTG AGCAAGGCGA TTTAACGTTT CAGCAAGGGG ATGTGATTTT GGTTACCAAG
    AAAGATGGTG ACTGGTGGAC AGGAGCAGTG GGCGACAAGT CCGGAGTCTT CCCTTCTAAC
    TATGTGAGGC TTAAAGATTC AGAGGGCTCT GGAACTGCTG GGAAAACAGG GAGTTTAGGA
    AAAAAACCTG AAATTGCGCA GGTTATCGCC TGCTACACAG CTACTGGTCC CGAGCAGCTC
    ACCCTGGCCC CTGGTCAGCT GATTTTGATC CGAAAAAAGA ACCCAGGCGG TTGGTGGGAA
    GGAGAGCTGC AAGCCCGTGG GAAGAAGCGC CAGATAGGCT GGTTCCCCGC GAATTATGTA
    AAACTTTTGA GCCCTGGCAC GAGCAAAATC ACTCCAACGG AGCCACCTAA GCCCACGGCG
    TTCCCGGCAG TGTGCCAGGT GATCGGCGTG TACGACTACA GCGCCCAGAA CGACGACGAG
    CTGGCCTTCA GCAAGGGCCA GATCATCAAC GTCCTCAACA AAGACGACCC GGACTGGTGG
    AAGGGCGAGG TCAACGGGCA AGTGGGGCTC TTCCCCTCCA ACTACGTGAA GCTGACCACG
    GACATGGACC CAAGCCAGCA ATGGTGCTCA GACTTACACC TCTTGGATAT GTTGACCCCA
    ACGGAGAGAA AGCGACAAGG ATACATCCAT GAGCTCATTG TCACAGAGGA GAACTATGTG
    AATGACCTGC AGCTGGTCAC AGAGATCTTT CAGAAACCCC TGATGGAGTC TGAGCTGCTG
    ACAGAAAAAG AGGGTGCTAT GATTTTTGTT AACTGGAAGG AGCTGATTAT GTGTAATATC
    AAACTATTGA AAGCGCTGAG GGTCCGCAAG AAGATGTCGG GGGAGAAGAT GCCCGTGAAG
    ATGATCGGCG ACATCCTGAC GGCGCAGCTG CCGCACATGC AGCCTTACAT CCGCTTCTGC
    AGCTGTCAGC TCAACGGGGC TGCCCTCATC CAGCAGAAGA CCGACGAGGC CCCGGACTTC
    AAGGAGTTTG TCAAAAGGCT GGCAATGGAC CCTCGATGTA AAGGGATGCC ACTCTCTAGT
    TTTATCCTGA AGCCTATGCA ACGCGTAACG AGATACCCAC TGATCATTAA AAATATCCTG
    GAAAACACTC CTGAGAACCA CCCCGACCAC AGCCACCTGA AGCACGCCCT GGAGAAGGCC
    GAAGAGCTGT GTTCACAGGT GAACGAAGGG GTGCGCGAGA AGGAGAACTC CGACCGCCTC
    GAGTGGATCC AGGCGCACGT GCAGTGCGAA GGCCTGTCCG AGCAACTTGT GTTCAATTCA
    GTGACCAATT GCTTGGGGCC ACGCAAGTTT CTGCACAGTG GGAAACTCTA CAAGGCCAAG
    AGCAACAAGG AGCTGTATGG CTTCCTCTTC AACGACTTCC TCCTGCTGAC CCAAATCATA
    AAGCCCTTGG GCTCTTCCGG CACCGACAAA GTCTTCAGTC CCAAATCTAA CCTGCAGTAT
    AAAATGTACA AAACACCTAT TTTCCTGAAT GAGGTTCTAG TAAAATTACC CACTGACCCT
    TCTGGAGATG AGCCCATCTT CCACATCTCC CACATTGACC GAGTCTACAC CCTCCGAGCC
    GAAAGCATAA ATGAAAGGAC TGCCTGGGTG CAGAAAATCA AAGCTGCTTC AGAACTTTAT
    ATAGAGACTG AGAAGAAGAA GCGGGAGAAG GCGTACCTGG TCCGTTCCCA AAGGGCGACA
    GGTATTGGAA GGTTGATGGT GAACGTCGTT GAGGGCATTG AGCTGAAACC CTGTCGATCA
    CATGGAAAGA GTAACCCGTA CTGTGAGGTG ACCATGGGTT CCCAGTGTCA CATCACCAAG
    ACGATCCAGG ACACTCTGAA TCCCAAGTGG AATTCCAACT GCCAGTTCTT CATCAAAGAC
    CTGGAGCAGG AGGTCCTGTG CATCACCGTG TTTGAGAGGG ACCAGTTCTC TCCAGACGAT
    TTTTTGGGTC GGACAGAGAT CCGTGTGGCC GACATCAAGA AGGACCAGGG TTCCAAGGGT
    CCAGTTACAA AGTGTCTCCT GCTACACGAA GTTCCCACAG GAGAGATTGT GGTCCGCTTG
    GACCTGCAGC TGTTTGATGA GCCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44461
    Clone ID Related Accession (Same CDS sequence) XM_014109748.1 , XM_014109748.2
    Accession Version XM_014109748.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4953bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product intersectin-1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCTCAGT TTCCAACACC TTTTGGAGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAA 
    GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG
    ATATGGGCGC TAGCTGACAT GAACAATGAT GGACGAATGG ATCAAGTGGA GTTTTCCATA
    GCTATGAAAC TCATCAAACT CAAGCTGCAA GGATATCAAC TTCCTTCTGC ACTTCCCCCC
    GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CCTTTGGTAT AGGAGGTATT
    GCCAGCATGC CACCACTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTC
    GGAATGTCTC CACCTCTAGT ATCTTCTGTT CCTACAGCAG CAGTGCCTCC CCTGGCTAAC
    GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA
    AAGAGCTCTT CCTTCAGTAG ATCTGGTCCC GGGTCACAAT TAAACACTAA GTTACAGAAG
    GCACAGTCAT TTGATGTGGC TAGTGTCCCT CCCGTGGCAG AATGGGCTGT CCCTCAGTCA
    TCCAGACTTA AATACAGACA GTTATTCAAC AGTCATGACA AAACTATGAG TGGACACTTA
    ACAGGTCCAC AAGCAAGGAC TATTCTTATG CAATCAAGTT TGCCACAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC
    TTGGCTATGC ACCTAATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT
    CCAGAGTACA TTCCACCTTC CTTTAGACGA GTTCGATCTG GCAGTGGTAT ATCAGTCATC
    AGCTCAACAG CAGTAGATCA GAGGTTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA
    CAACTAGAAA AGAAATTACC TGTAACCTTT GAAGATAAGA AGCGTGAGAA TTTTGAACGT
    GGCAATCTGG AACTCGAGAA ACGGAGACAA GCGCTCTTGG AGCAGCAGCG CAAAGAGCAA
    GAGCGCCTGG CACAGCTTGA ACGTGCAGAG CAAGAACGGA AAGAGCGGGA GCGCCAGGAG
    CAAGAGCGCA AAAGGCAACT GGAGTTGGAG AAACAACTGG AAAAGCAACG GGAGCTAGAA
    CGTCAGAGAG AAGAGGAGAG GAGGAAAGAA ATTGAGAGAC GAGAGGCTGC AAAGAGAGAA
    CTTGAAAGAC AGCGGCAACT GGAATGGGAA CGAAATCGAA GACAAGAGCT ACTCAATCAG
    AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAGAAGAC TCTAGAATTT
    GAATTAGAAG CTCTTAATGA TAAAAAGCAT CAACTAGAAG GAAAACTTCA AGATATCAGG
    TGTCGATTGA CCACCCAACG GCAAGAAATT GAGAGTACAA ATAAATCCAG AGAGTTGAGA
    ATTGCTGAAA TCACCCATCT ACAGCAACAA TTACAGGAAG CCCAGCAAAT GCTTGGAAGA
    CTTATTCCAG AAAAGCAGAT ACTCAATGAC CAATTAAAAC AAGTTCAACA GAACAGTTTG
    CACAGAGATT CACTGCTTAC ACTCAAAAGA GCGTTAGAAG CAAAAGAACT AGCTCGGCAG
    CAGCTACGCG ATCAACTGGA TGAAGTTGAG AAAGAAACCA GATCAAAACT ACAGGAGATT
    GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ATAGTAAGCA ACAACTCCAG
    AAACAAAAGT CCATAGAGGC CGAACGTCTG AAACAGAAAG AACAAGAGCG AAAAACCATA
    GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAGAGGGA CAAGCAGTGG
    ATTGAGCATG TGCAGCAGGA CGATGAGCAT CAGCGGCCAA GGAAGATCCA TGATGAAGAA
    AAACTCCGAA GGGAAGAGAG TGTCAAAAAG AAGGACAGTG AGGAAAAAAG CAAACTGGAC
    ACGCAAGACA AGATGAGTCG GCTTTTCCAC CAGCATCAAG AGCCAGCCAA GCCAGCTGTC
    CAGGCACCCT GGTCCACCAC AGAAAAAGGC CCACTTACAA TTTCTGCACA GGAGAACGTA
    AAGGTGGTGT ATTACCGAGC GCTCTATCCC TTCGAATCCA GAAGTCATGA TGAGATCACG
    ATCCAGCCTG GAGACATAGT AATGGTTAAA GGGGAATGGG TGGATGAAAG CCAAACTGGA
    GAACCGGGCT GGCTTGGAGG AGAATTAAAA GGAAAGACGG GATGGTTCCC TGCTAACTAC
    GCAGAGAAAA TCCCAGAAAA CGAGGTGCCC GCTCCAGTCA AACCCGGGCC CGACCTCACC
    TCTGCCCCTG CGCCCAAACT GGCCGTGCGT GAGACCCCCG CTCCGGTGGC AGTGAGCGCT
    CCGGAGCCCT CCACGACCCC CAACAGCTGG GCTGACTTCA GCTCCACGTG GCCCACCAGC
    ACGAATGAGA AGCCAGAAAC CGACAACTGG GACGCATGGG CAGCCCAGCC CTCACTCACT
    GTTCCCAGTG CTGGCCAGCT AAGGCAGAGA TCAGCGTTTA CTCCAGCCAC GGCCACTGGC
    TCCTCTCCAT CTCCCGTTTT AGGCCAGGGT GAGAAGGTGG AAGGGCTACA AGCACAAGCC
    CTGTATCCTT GGAGAGCCAA AAAAGACAAC CACTTAAATT TTAACAAAAA TGACGTCATC
    ACCGTCCTGG AACAGCAAGA CATGTGGTGG TTTGGAGAAG TCCAAGGTCA GAAGGGTTGG
    TTCCCCAAGT CTTACGTGAA ACTCATTTCA GGGCCCATAA GGAAATCCAC AAGCATGGAT
    TCTGGTTCTT CAGAAAGTCC CGCTAGTCTA AAGAGAGTAG CTTCCCCAGC AGCCAAGCCG
    GCCATTTCGG GAGAAGAAAT TGCGCAGGTT ATCGCCTGCT ACACAGCTAC TGGTCCCGAG
    CAGCTCACCC TGGCCCCTGG TCAGCTGATT TTGATCCGAA AAAAGAACCC AGGCGGTTGG
    TGGGAAGGAG AGCTGCAAGC CCGTGGGAAG AAGCGCCAGA TAGGCTGGTT CCCCGCGAAT
    TATGTAAAAC TTTTGAGCCC TGGCACGAGC AAAATCACTC CAACGGAGCC ACCTAAGCCC
    ACGGCGTTCC CGGCAGTGTG CCAGGTGATC GGCGTGTACG ACTACAGCGC CCAGAACGAC
    GACGAGCTGG CCTTCAGCAA GGGCCAGATC ATCAACGTCC TCAACAAAGA CGACCCGGAC
    TGGTGGAAGG GCGAGGTCAA CGGGCAAGTG GGGCTCTTCC CCTCCAACTA CGTGAAGCTG
    ACCACGGACA TGGACCCAAG CCAGCAATGG TGCTCAGACT TACACCTCTT GGATATGTTG
    ACCCCAACGG AGAGAAAGCG ACAAGGATAC ATCCATGAGC TCATTGTCAC AGAGGAGAAC
    TATGTGAATG ACCTGCAGCT GGTCACAGAG ATCTTTCAGA AACCCCTGAT GGAGTCTGAG
    CTGCTGACAG AAAAAGAGGG TGCTATGATT TTTGTTAACT GGAAGGAGCT GATTATGTGT
    AATATCAAAC TATTGAAAGC GCTGAGGGTC CGCAAGAAGA TGTCGGGGGA GAAGATGCCC
    GTGAAGATGA TCGGCGACAT CCTGACGGCG CAGCTGCCGC ACATGCAGCC TTACATCCGC
    TTCTGCAGCT GTCAGCTCAA CGGGGCTGCC CTCATCCAGC AGAAGACCGA CGAGGCCCCG
    GACTTCAAGG AGTTTGTCAA AAGGCTGGCA ATGGACCCTC GATGTAAAGG GATGCCACTC
    TCTAGTTTTA TCCTGAAGCC TATGCAACGC GTAACGAGAT ACCCACTGAT CATTAAAAAT
    ATCCTGGAAA ACACTCCTGA GAACCACCCC GACCACAGCC ACCTGAAGCA CGCCCTGGAG
    AAGGCCGAAG AGCTGTGTTC ACAGGTGAAC GAAGGGGTGC GCGAGAAGGA GAACTCCGAC
    CGCCTCGAGT GGATCCAGGC GCACGTGCAG TGCGAAGGCC TGTCCGAGCA ACTTGTGTTC
    AATTCAGTGA CCAATTGCTT GGGGCCACGC AAGTTTCTGC ACAGTGGGAA ACTCTACAAG
    GCCAAGAGCA ACAAGGAGCT GTATGGCTTC CTCTTCAACG ACTTCCTCCT GCTGACCCAA
    ATCATAAAGC CCTTGGGCTC TTCCGGCACC GACAAAGTCT TCAGTCCCAA ATCTAACCTG
    CAGTATAAAA TGTACAAAAC ACCTATTTTC CTGAATGAGG TTCTAGTAAA ATTACCCACT
    GACCCTTCTG GAGATGAGCC CATCTTCCAC ATCTCCCACA TTGACCGAGT CTACACCCTC
    CGAGCCGAAA GCATAAATGA AAGGACTGCC TGGGTGCAGA AAATCAAAGC TGCTTCAGAA
    CTTTATATAG AGACTGAGAA GAAGAAGCGG GAGAAGGCGT ACCTGGTCCG TTCCCAAAGG
    GCGACAGGTA TTGGAAGGTT GATGGTGAAC GTCGTTGAGG GCATTGAGCT GAAACCCTGT
    CGATCACATG GAAAGAGTAA CCCGTACTGT GAGGTGACCA TGGGTTCCCA GTGTCACATC
    ACCAAGACGA TCCAGGACAC TCTGAATCCC AAGTGGAATT CCAACTGCCA GTTCTTCATC
    AAAGACCTGG AGCAGGAGGT CCTGTGCATC ACCGTGTTTG AGAGGGACCA GTTCTCTCCA
    GACGATTTTT TGGGTCGGAC AGAGATCCGT GTGGCCGACA TCAAGAAGGA CCAGGGTTCC
    AAGGGTCCAG TTACAAAGTG TCTCCTGCTA CACGAAGTTC CCACAGGAGA GATTGTGGTC
    CGCTTGGACC TGCAGCTGTT TGATGAGCCA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965223.1
    CDS298..5250
    Translation

    Target ORF information:

    RefSeq Version XM_014109748.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109748.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCTCAGT TTCCAACACC TTTTGGAGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAA 
    GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG
    ATATGGGCGC TAGCTGACAT GAACAATGAT GGACGAATGG ATCAAGTGGA GTTTTCCATA
    GCTATGAAAC TCATCAAACT CAAGCTGCAA GGATATCAAC TTCCTTCTGC ACTTCCCCCC
    GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CCTTTGGTAT AGGAGGTATT
    GCCAGCATGC CACCACTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTC
    GGAATGTCTC CACCTCTAGT ATCTTCTGTT CCTACAGCAG CAGTGCCTCC CCTGGCTAAC
    GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA
    AAGAGCTCTT CCTTCAGTAG ATCTGGTCCC GGGTCACAAT TAAACACTAA GTTACAGAAG
    GCACAGTCAT TTGATGTGGC TAGTGTCCCT CCCGTGGCAG AATGGGCTGT CCCTCAGTCA
    TCCAGACTTA AATACAGACA GTTATTCAAC AGTCATGACA AAACTATGAG TGGACACTTA
    ACAGGTCCAC AAGCAAGGAC TATTCTTATG CAATCAAGTT TGCCACAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC
    TTGGCTATGC ACCTAATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT
    CCAGAGTACA TTCCACCTTC CTTTAGACGA GTTCGATCTG GCAGTGGTAT ATCAGTCATC
    AGCTCAACAG CAGTAGATCA GAGGTTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA
    CAACTAGAAA AGAAATTACC TGTAACCTTT GAAGATAAGA AGCGTGAGAA TTTTGAACGT
    GGCAATCTGG AACTCGAGAA ACGGAGACAA GCGCTCTTGG AGCAGCAGCG CAAAGAGCAA
    GAGCGCCTGG CACAGCTTGA ACGTGCAGAG CAAGAACGGA AAGAGCGGGA GCGCCAGGAG
    CAAGAGCGCA AAAGGCAACT GGAGTTGGAG AAACAACTGG AAAAGCAACG GGAGCTAGAA
    CGTCAGAGAG AAGAGGAGAG GAGGAAAGAA ATTGAGAGAC GAGAGGCTGC AAAGAGAGAA
    CTTGAAAGAC AGCGGCAACT GGAATGGGAA CGAAATCGAA GACAAGAGCT ACTCAATCAG
    AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAGAAGAC TCTAGAATTT
    GAATTAGAAG CTCTTAATGA TAAAAAGCAT CAACTAGAAG GAAAACTTCA AGATATCAGG
    TGTCGATTGA CCACCCAACG GCAAGAAATT GAGAGTACAA ATAAATCCAG AGAGTTGAGA
    ATTGCTGAAA TCACCCATCT ACAGCAACAA TTACAGGAAG CCCAGCAAAT GCTTGGAAGA
    CTTATTCCAG AAAAGCAGAT ACTCAATGAC CAATTAAAAC AAGTTCAACA GAACAGTTTG
    CACAGAGATT CACTGCTTAC ACTCAAAAGA GCGTTAGAAG CAAAAGAACT AGCTCGGCAG
    CAGCTACGCG ATCAACTGGA TGAAGTTGAG AAAGAAACCA GATCAAAACT ACAGGAGATT
    GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ATAGTAAGCA ACAACTCCAG
    AAACAAAAGT CCATAGAGGC CGAACGTCTG AAACAGAAAG AACAAGAGCG AAAAACCATA
    GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAGAGGGA CAAGCAGTGG
    ATTGAGCATG TGCAGCAGGA CGATGAGCAT CAGCGGCCAA GGAAGATCCA TGATGAAGAA
    AAACTCCGAA GGGAAGAGAG TGTCAAAAAG AAGGACAGTG AGGAAAAAAG CAAACTGGAC
    ACGCAAGACA AGATGAGTCG GCTTTTCCAC CAGCATCAAG AGCCAGCCAA GCCAGCTGTC
    CAGGCACCCT GGTCCACCAC AGAAAAAGGC CCACTTACAA TTTCTGCACA GGAGAACGTA
    AAGGTGGTGT ATTACCGAGC GCTCTATCCC TTCGAATCCA GAAGTCATGA TGAGATCACG
    ATCCAGCCTG GAGACATAGT AATGGTTAAA GGGGAATGGG TGGATGAAAG CCAAACTGGA
    GAACCGGGCT GGCTTGGAGG AGAATTAAAA GGAAAGACGG GATGGTTCCC TGCTAACTAC
    GCAGAGAAAA TCCCAGAAAA CGAGGTGCCC GCTCCAGTCA AACCCGGGCC CGACCTCACC
    TCTGCCCCTG CGCCCAAACT GGCCGTGCGT GAGACCCCCG CTCCGGTGGC AGTGAGCGCT
    CCGGAGCCCT CCACGACCCC CAACAGCTGG GCTGACTTCA GCTCCACGTG GCCCACCAGC
    ACGAATGAGA AGCCAGAAAC CGACAACTGG GACGCATGGG CAGCCCAGCC CTCACTCACT
    GTTCCCAGTG CTGGCCAGCT AAGGCAGAGA TCAGCGTTTA CTCCAGCCAC GGCCACTGGC
    TCCTCTCCAT CTCCCGTTTT AGGCCAGGGT GAGAAGGTGG AAGGGCTACA AGCACAAGCC
    CTGTATCCTT GGAGAGCCAA AAAAGACAAC CACTTAAATT TTAACAAAAA TGACGTCATC
    ACCGTCCTGG AACAGCAAGA CATGTGGTGG TTTGGAGAAG TCCAAGGTCA GAAGGGTTGG
    TTCCCCAAGT CTTACGTGAA ACTCATTTCA GGGCCCATAA GGAAATCCAC AAGCATGGAT
    TCTGGTTCTT CAGAAAGTCC CGCTAGTCTA AAGAGAGTAG CTTCCCCAGC AGCCAAGCCG
    GCCATTTCGG GAGAAGAAAT TGCGCAGGTT ATCGCCTGCT ACACAGCTAC TGGTCCCGAG
    CAGCTCACCC TGGCCCCTGG TCAGCTGATT TTGATCCGAA AAAAGAACCC AGGCGGTTGG
    TGGGAAGGAG AGCTGCAAGC CCGTGGGAAG AAGCGCCAGA TAGGCTGGTT CCCCGCGAAT
    TATGTAAAAC TTTTGAGCCC TGGCACGAGC AAAATCACTC CAACGGAGCC ACCTAAGCCC
    ACGGCGTTCC CGGCAGTGTG CCAGGTGATC GGCGTGTACG ACTACAGCGC CCAGAACGAC
    GACGAGCTGG CCTTCAGCAA GGGCCAGATC ATCAACGTCC TCAACAAAGA CGACCCGGAC
    TGGTGGAAGG GCGAGGTCAA CGGGCAAGTG GGGCTCTTCC CCTCCAACTA CGTGAAGCTG
    ACCACGGACA TGGACCCAAG CCAGCAATGG TGCTCAGACT TACACCTCTT GGATATGTTG
    ACCCCAACGG AGAGAAAGCG ACAAGGATAC ATCCATGAGC TCATTGTCAC AGAGGAGAAC
    TATGTGAATG ACCTGCAGCT GGTCACAGAG ATCTTTCAGA AACCCCTGAT GGAGTCTGAG
    CTGCTGACAG AAAAAGAGGG TGCTATGATT TTTGTTAACT GGAAGGAGCT GATTATGTGT
    AATATCAAAC TATTGAAAGC GCTGAGGGTC CGCAAGAAGA TGTCGGGGGA GAAGATGCCC
    GTGAAGATGA TCGGCGACAT CCTGACGGCG CAGCTGCCGC ACATGCAGCC TTACATCCGC
    TTCTGCAGCT GTCAGCTCAA CGGGGCTGCC CTCATCCAGC AGAAGACCGA CGAGGCCCCG
    GACTTCAAGG AGTTTGTCAA AAGGCTGGCA ATGGACCCTC GATGTAAAGG GATGCCACTC
    TCTAGTTTTA TCCTGAAGCC TATGCAACGC GTAACGAGAT ACCCACTGAT CATTAAAAAT
    ATCCTGGAAA ACACTCCTGA GAACCACCCC GACCACAGCC ACCTGAAGCA CGCCCTGGAG
    AAGGCCGAAG AGCTGTGTTC ACAGGTGAAC GAAGGGGTGC GCGAGAAGGA GAACTCCGAC
    CGCCTCGAGT GGATCCAGGC GCACGTGCAG TGCGAAGGCC TGTCCGAGCA ACTTGTGTTC
    AATTCAGTGA CCAATTGCTT GGGGCCACGC AAGTTTCTGC ACAGTGGGAA ACTCTACAAG
    GCCAAGAGCA ACAAGGAGCT GTATGGCTTC CTCTTCAACG ACTTCCTCCT GCTGACCCAA
    ATCATAAAGC CCTTGGGCTC TTCCGGCACC GACAAAGTCT TCAGTCCCAA ATCTAACCTG
    CAGTATAAAA TGTACAAAAC ACCTATTTTC CTGAATGAGG TTCTAGTAAA ATTACCCACT
    GACCCTTCTG GAGATGAGCC CATCTTCCAC ATCTCCCACA TTGACCGAGT CTACACCCTC
    CGAGCCGAAA GCATAAATGA AAGGACTGCC TGGGTGCAGA AAATCAAAGC TGCTTCAGAA
    CTTTATATAG AGACTGAGAA GAAGAAGCGG GAGAAGGCGT ACCTGGTCCG TTCCCAAAGG
    GCGACAGGTA TTGGAAGGTT GATGGTGAAC GTCGTTGAGG GCATTGAGCT GAAACCCTGT
    CGATCACATG GAAAGAGTAA CCCGTACTGT GAGGTGACCA TGGGTTCCCA GTGTCACATC
    ACCAAGACGA TCCAGGACAC TCTGAATCCC AAGTGGAATT CCAACTGCCA GTTCTTCATC
    AAAGACCTGG AGCAGGAGGT CCTGTGCATC ACCGTGTTTG AGAGGGACCA GTTCTCTCCA
    GACGATTTTT TGGGTCGGAC AGAGATCCGT GTGGCCGACA TCAAGAAGGA CCAGGGTTCC
    AAGGGTCCAG TTACAAAGTG TCTCCTGCTA CACGAAGTTC CCACAGGAGA GATTGTGGTC
    CGCTTGGACC TGCAGCTGTT TGATGAGCCA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe44461
    Clone ID Related Accession (Same CDS sequence) XM_014109748.1 , XM_014109748.2
    Accession Version XM_014109748.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4953bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product intersectin-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109748.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCTCAGT TTCCAACACC TTTTGGAGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAA 
    GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG
    ATATGGGCGC TAGCTGACAT GAACAATGAT GGACGAATGG ATCAAGTGGA GTTTTCCATA
    GCTATGAAAC TCATCAAACT CAAGCTGCAA GGATATCAAC TTCCTTCTGC ACTTCCCCCC
    GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CCTTTGGTAT AGGAGGTATT
    GCCAGCATGC CACCACTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTC
    GGAATGTCTC CACCTCTAGT ATCTTCTGTT CCTACAGCAG CAGTGCCTCC CCTGGCTAAC
    GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA
    AAGAGCTCTT CCTTCAGTAG ATCTGGTCCC GGGTCACAAT TAAACACTAA GTTACAGAAG
    GCACAGTCAT TTGATGTGGC TAGTGTCCCT CCCGTGGCAG AATGGGCTGT CCCTCAGTCA
    TCCAGACTTA AATACAGACA GTTATTCAAC AGTCATGACA AAACTATGAG TGGACACTTA
    ACAGGTCCAC AAGCAAGGAC TATTCTTATG CAATCAAGTT TGCCACAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC
    TTGGCTATGC ACCTAATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT
    CCAGAGTACA TTCCACCTTC CTTTAGACGA GTTCGATCTG GCAGTGGTAT ATCAGTCATC
    AGCTCAACAG CAGTAGATCA GAGGTTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA
    CAACTAGAAA AGAAATTACC TGTAACCTTT GAAGATAAGA AGCGTGAGAA TTTTGAACGT
    GGCAATCTGG AACTCGAGAA ACGGAGACAA GCGCTCTTGG AGCAGCAGCG CAAAGAGCAA
    GAGCGCCTGG CACAGCTTGA ACGTGCAGAG CAAGAACGGA AAGAGCGGGA GCGCCAGGAG
    CAAGAGCGCA AAAGGCAACT GGAGTTGGAG AAACAACTGG AAAAGCAACG GGAGCTAGAA
    CGTCAGAGAG AAGAGGAGAG GAGGAAAGAA ATTGAGAGAC GAGAGGCTGC AAAGAGAGAA
    CTTGAAAGAC AGCGGCAACT GGAATGGGAA CGAAATCGAA GACAAGAGCT ACTCAATCAG
    AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAGAAGAC TCTAGAATTT
    GAATTAGAAG CTCTTAATGA TAAAAAGCAT CAACTAGAAG GAAAACTTCA AGATATCAGG
    TGTCGATTGA CCACCCAACG GCAAGAAATT GAGAGTACAA ATAAATCCAG AGAGTTGAGA
    ATTGCTGAAA TCACCCATCT ACAGCAACAA TTACAGGAAG CCCAGCAAAT GCTTGGAAGA
    CTTATTCCAG AAAAGCAGAT ACTCAATGAC CAATTAAAAC AAGTTCAACA GAACAGTTTG
    CACAGAGATT CACTGCTTAC ACTCAAAAGA GCGTTAGAAG CAAAAGAACT AGCTCGGCAG
    CAGCTACGCG ATCAACTGGA TGAAGTTGAG AAAGAAACCA GATCAAAACT ACAGGAGATT
    GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ATAGTAAGCA ACAACTCCAG
    AAACAAAAGT CCATAGAGGC CGAACGTCTG AAACAGAAAG AACAAGAGCG AAAAACCATA
    GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAGAGGGA CAAGCAGTGG
    ATTGAGCATG TGCAGCAGGA CGATGAGCAT CAGCGGCCAA GGAAGATCCA TGATGAAGAA
    AAACTCCGAA GGGAAGAGAG TGTCAAAAAG AAGGACAGTG AGGAAAAAAG CAAACTGGAC
    ACGCAAGACA AGATGAGTCG GCTTTTCCAC CAGCATCAAG AGCCAGCCAA GCCAGCTGTC
    CAGGCACCCT GGTCCACCAC AGAAAAAGGC CCACTTACAA TTTCTGCACA GGAGAACGTA
    AAGGTGGTGT ATTACCGAGC GCTCTATCCC TTCGAATCCA GAAGTCATGA TGAGATCACG
    ATCCAGCCTG GAGACATAGT AATGGTTAAA GGGGAATGGG TGGATGAAAG CCAAACTGGA
    GAACCGGGCT GGCTTGGAGG AGAATTAAAA GGAAAGACGG GATGGTTCCC TGCTAACTAC
    GCAGAGAAAA TCCCAGAAAA CGAGGTGCCC GCTCCAGTCA AACCCGGGCC CGACCTCACC
    TCTGCCCCTG CGCCCAAACT GGCCGTGCGT GAGACCCCCG CTCCGGTGGC AGTGAGCGCT
    CCGGAGCCCT CCACGACCCC CAACAGCTGG GCTGACTTCA GCTCCACGTG GCCCACCAGC
    ACGAATGAGA AGCCAGAAAC CGACAACTGG GACGCATGGG CAGCCCAGCC CTCACTCACT
    GTTCCCAGTG CTGGCCAGCT AAGGCAGAGA TCAGCGTTTA CTCCAGCCAC GGCCACTGGC
    TCCTCTCCAT CTCCCGTTTT AGGCCAGGGT GAGAAGGTGG AAGGGCTACA AGCACAAGCC
    CTGTATCCTT GGAGAGCCAA AAAAGACAAC CACTTAAATT TTAACAAAAA TGACGTCATC
    ACCGTCCTGG AACAGCAAGA CATGTGGTGG TTTGGAGAAG TCCAAGGTCA GAAGGGTTGG
    TTCCCCAAGT CTTACGTGAA ACTCATTTCA GGGCCCATAA GGAAATCCAC AAGCATGGAT
    TCTGGTTCTT CAGAAAGTCC CGCTAGTCTA AAGAGAGTAG CTTCCCCAGC AGCCAAGCCG
    GCCATTTCGG GAGAAGAAAT TGCGCAGGTT ATCGCCTGCT ACACAGCTAC TGGTCCCGAG
    CAGCTCACCC TGGCCCCTGG TCAGCTGATT TTGATCCGAA AAAAGAACCC AGGCGGTTGG
    TGGGAAGGAG AGCTGCAAGC CCGTGGGAAG AAGCGCCAGA TAGGCTGGTT CCCCGCGAAT
    TATGTAAAAC TTTTGAGCCC TGGCACGAGC AAAATCACTC CAACGGAGCC ACCTAAGCCC
    ACGGCGTTCC CGGCAGTGTG CCAGGTGATC GGCGTGTACG ACTACAGCGC CCAGAACGAC
    GACGAGCTGG CCTTCAGCAA GGGCCAGATC ATCAACGTCC TCAACAAAGA CGACCCGGAC
    TGGTGGAAGG GCGAGGTCAA CGGGCAAGTG GGGCTCTTCC CCTCCAACTA CGTGAAGCTG
    ACCACGGACA TGGACCCAAG CCAGCAATGG TGCTCAGACT TACACCTCTT GGATATGTTG
    ACCCCAACGG AGAGAAAGCG ACAAGGATAC ATCCATGAGC TCATTGTCAC AGAGGAGAAC
    TATGTGAATG ACCTGCAGCT GGTCACAGAG ATCTTTCAGA AACCCCTGAT GGAGTCTGAG
    CTGCTGACAG AAAAAGAGGG TGCTATGATT TTTGTTAACT GGAAGGAGCT GATTATGTGT
    AATATCAAAC TATTGAAAGC GCTGAGGGTC CGCAAGAAGA TGTCGGGGGA GAAGATGCCC
    GTGAAGATGA TCGGCGACAT CCTGACGGCG CAGCTGCCGC ACATGCAGCC TTACATCCGC
    TTCTGCAGCT GTCAGCTCAA CGGGGCTGCC CTCATCCAGC AGAAGACCGA CGAGGCCCCG
    GACTTCAAGG AGTTTGTCAA AAGGCTGGCA ATGGACCCTC GATGTAAAGG GATGCCACTC
    TCTAGTTTTA TCCTGAAGCC TATGCAACGC GTAACGAGAT ACCCACTGAT CATTAAAAAT
    ATCCTGGAAA ACACTCCTGA GAACCACCCC GACCACAGCC ACCTGAAGCA CGCCCTGGAG
    AAGGCCGAAG AGCTGTGTTC ACAGGTGAAC GAAGGGGTGC GCGAGAAGGA GAACTCCGAC
    CGCCTCGAGT GGATCCAGGC GCACGTGCAG TGCGAAGGCC TGTCCGAGCA ACTTGTGTTC
    AATTCAGTGA CCAATTGCTT GGGGCCACGC AAGTTTCTGC ACAGTGGGAA ACTCTACAAG
    GCCAAGAGCA ACAAGGAGCT GTATGGCTTC CTCTTCAACG ACTTCCTCCT GCTGACCCAA
    ATCATAAAGC CCTTGGGCTC TTCCGGCACC GACAAAGTCT TCAGTCCCAA ATCTAACCTG
    CAGTATAAAA TGTACAAAAC ACCTATTTTC CTGAATGAGG TTCTAGTAAA ATTACCCACT
    GACCCTTCTG GAGATGAGCC CATCTTCCAC ATCTCCCACA TTGACCGAGT CTACACCCTC
    CGAGCCGAAA GCATAAATGA AAGGACTGCC TGGGTGCAGA AAATCAAAGC TGCTTCAGAA
    CTTTATATAG AGACTGAGAA GAAGAAGCGG GAGAAGGCGT ACCTGGTCCG TTCCCAAAGG
    GCGACAGGTA TTGGAAGGTT GATGGTGAAC GTCGTTGAGG GCATTGAGCT GAAACCCTGT
    CGATCACATG GAAAGAGTAA CCCGTACTGT GAGGTGACCA TGGGTTCCCA GTGTCACATC
    ACCAAGACGA TCCAGGACAC TCTGAATCCC AAGTGGAATT CCAACTGCCA GTTCTTCATC
    AAAGACCTGG AGCAGGAGGT CCTGTGCATC ACCGTGTTTG AGAGGGACCA GTTCTCTCCA
    GACGATTTTT TGGGTCGGAC AGAGATCCGT GTGGCCGACA TCAAGAAGGA CCAGGGTTCC
    AAGGGTCCAG TTACAAAGTG TCTCCTGCTA CACGAAGTTC CCACAGGAGA GATTGTGGTC
    CGCTTGGACC TGCAGCTGTT TGATGAGCCA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965223.1
    CDS259..5211
    Translation

    Target ORF information:

    RefSeq Version XM_014109748.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris intersectin 1 (ITSN1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109748.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCTCAGT TTCCAACACC TTTTGGAGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAA 
    GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG
    ATATGGGCGC TAGCTGACAT GAACAATGAT GGACGAATGG ATCAAGTGGA GTTTTCCATA
    GCTATGAAAC TCATCAAACT CAAGCTGCAA GGATATCAAC TTCCTTCTGC ACTTCCCCCC
    GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CCTTTGGTAT AGGAGGTATT
    GCCAGCATGC CACCACTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTC
    GGAATGTCTC CACCTCTAGT ATCTTCTGTT CCTACAGCAG CAGTGCCTCC CCTGGCTAAC
    GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA
    AAGAGCTCTT CCTTCAGTAG ATCTGGTCCC GGGTCACAAT TAAACACTAA GTTACAGAAG
    GCACAGTCAT TTGATGTGGC TAGTGTCCCT CCCGTGGCAG AATGGGCTGT CCCTCAGTCA
    TCCAGACTTA AATACAGACA GTTATTCAAC AGTCATGACA AAACTATGAG TGGACACTTA
    ACAGGTCCAC AAGCAAGGAC TATTCTTATG CAATCAAGTT TGCCACAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC
    TTGGCTATGC ACCTAATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT
    CCAGAGTACA TTCCACCTTC CTTTAGACGA GTTCGATCTG GCAGTGGTAT ATCAGTCATC
    AGCTCAACAG CAGTAGATCA GAGGTTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA
    CAACTAGAAA AGAAATTACC TGTAACCTTT GAAGATAAGA AGCGTGAGAA TTTTGAACGT
    GGCAATCTGG AACTCGAGAA ACGGAGACAA GCGCTCTTGG AGCAGCAGCG CAAAGAGCAA
    GAGCGCCTGG CACAGCTTGA ACGTGCAGAG CAAGAACGGA AAGAGCGGGA GCGCCAGGAG
    CAAGAGCGCA AAAGGCAACT GGAGTTGGAG AAACAACTGG AAAAGCAACG GGAGCTAGAA
    CGTCAGAGAG AAGAGGAGAG GAGGAAAGAA ATTGAGAGAC GAGAGGCTGC AAAGAGAGAA
    CTTGAAAGAC AGCGGCAACT GGAATGGGAA CGAAATCGAA GACAAGAGCT ACTCAATCAG
    AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAGAAGAC TCTAGAATTT
    GAATTAGAAG CTCTTAATGA TAAAAAGCAT CAACTAGAAG GAAAACTTCA AGATATCAGG
    TGTCGATTGA CCACCCAACG GCAAGAAATT GAGAGTACAA ATAAATCCAG AGAGTTGAGA
    ATTGCTGAAA TCACCCATCT ACAGCAACAA TTACAGGAAG CCCAGCAAAT GCTTGGAAGA
    CTTATTCCAG AAAAGCAGAT ACTCAATGAC CAATTAAAAC AAGTTCAACA GAACAGTTTG
    CACAGAGATT CACTGCTTAC ACTCAAAAGA GCGTTAGAAG CAAAAGAACT AGCTCGGCAG
    CAGCTACGCG ATCAACTGGA TGAAGTTGAG AAAGAAACCA GATCAAAACT ACAGGAGATT
    GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ATAGTAAGCA ACAACTCCAG
    AAACAAAAGT CCATAGAGGC CGAACGTCTG AAACAGAAAG AACAAGAGCG AAAAACCATA
    GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAGAGGGA CAAGCAGTGG
    ATTGAGCATG TGCAGCAGGA CGATGAGCAT CAGCGGCCAA GGAAGATCCA TGATGAAGAA
    AAACTCCGAA GGGAAGAGAG TGTCAAAAAG AAGGACAGTG AGGAAAAAAG CAAACTGGAC
    ACGCAAGACA AGATGAGTCG GCTTTTCCAC CAGCATCAAG AGCCAGCCAA GCCAGCTGTC
    CAGGCACCCT GGTCCACCAC AGAAAAAGGC CCACTTACAA TTTCTGCACA GGAGAACGTA
    AAGGTGGTGT ATTACCGAGC GCTCTATCCC TTCGAATCCA GAAGTCATGA TGAGATCACG
    ATCCAGCCTG GAGACATAGT AATGGTTAAA GGGGAATGGG TGGATGAAAG CCAAACTGGA
    GAACCGGGCT GGCTTGGAGG AGAATTAAAA GGAAAGACGG GATGGTTCCC TGCTAACTAC
    GCAGAGAAAA TCCCAGAAAA CGAGGTGCCC GCTCCAGTCA AACCCGGGCC CGACCTCACC
    TCTGCCCCTG CGCCCAAACT GGCCGTGCGT GAGACCCCCG CTCCGGTGGC AGTGAGCGCT
    CCGGAGCCCT CCACGACCCC CAACAGCTGG GCTGACTTCA GCTCCACGTG GCCCACCAGC
    ACGAATGAGA AGCCAGAAAC CGACAACTGG GACGCATGGG CAGCCCAGCC CTCACTCACT
    GTTCCCAGTG CTGGCCAGCT AAGGCAGAGA TCAGCGTTTA CTCCAGCCAC GGCCACTGGC
    TCCTCTCCAT CTCCCGTTTT AGGCCAGGGT GAGAAGGTGG AAGGGCTACA AGCACAAGCC
    CTGTATCCTT GGAGAGCCAA AAAAGACAAC CACTTAAATT TTAACAAAAA TGACGTCATC
    ACCGTCCTGG AACAGCAAGA CATGTGGTGG TTTGGAGAAG TCCAAGGTCA GAAGGGTTGG
    TTCCCCAAGT CTTACGTGAA ACTCATTTCA GGGCCCATAA GGAAATCCAC AAGCATGGAT
    TCTGGTTCTT CAGAAAGTCC CGCTAGTCTA AAGAGAGTAG CTTCCCCAGC AGCCAAGCCG
    GCCATTTCGG GAGAAGAAAT TGCGCAGGTT ATCGCCTGCT ACACAGCTAC TGGTCCCGAG
    CAGCTCACCC TGGCCCCTGG TCAGCTGATT TTGATCCGAA AAAAGAACCC AGGCGGTTGG
    TGGGAAGGAG AGCTGCAAGC CCGTGGGAAG AAGCGCCAGA TAGGCTGGTT CCCCGCGAAT
    TATGTAAAAC TTTTGAGCCC TGGCACGAGC AAAATCACTC CAACGGAGCC ACCTAAGCCC
    ACGGCGTTCC CGGCAGTGTG CCAGGTGATC GGCGTGTACG ACTACAGCGC CCAGAACGAC
    GACGAGCTGG CCTTCAGCAA GGGCCAGATC ATCAACGTCC TCAACAAAGA CGACCCGGAC
    TGGTGGAAGG GCGAGGTCAA CGGGCAAGTG GGGCTCTTCC CCTCCAACTA CGTGAAGCTG
    ACCACGGACA TGGACCCAAG CCAGCAATGG TGCTCAGACT TACACCTCTT GGATATGTTG
    ACCCCAACGG AGAGAAAGCG ACAAGGATAC ATCCATGAGC TCATTGTCAC AGAGGAGAAC
    TATGTGAATG ACCTGCAGCT GGTCACAGAG ATCTTTCAGA AACCCCTGAT GGAGTCTGAG
    CTGCTGACAG AAAAAGAGGG TGCTATGATT TTTGTTAACT GGAAGGAGCT GATTATGTGT
    AATATCAAAC TATTGAAAGC GCTGAGGGTC CGCAAGAAGA TGTCGGGGGA GAAGATGCCC
    GTGAAGATGA TCGGCGACAT CCTGACGGCG CAGCTGCCGC ACATGCAGCC TTACATCCGC
    TTCTGCAGCT GTCAGCTCAA CGGGGCTGCC CTCATCCAGC AGAAGACCGA CGAGGCCCCG
    GACTTCAAGG AGTTTGTCAA AAGGCTGGCA ATGGACCCTC GATGTAAAGG GATGCCACTC
    TCTAGTTTTA TCCTGAAGCC TATGCAACGC GTAACGAGAT ACCCACTGAT CATTAAAAAT
    ATCCTGGAAA ACACTCCTGA GAACCACCCC GACCACAGCC ACCTGAAGCA CGCCCTGGAG
    AAGGCCGAAG AGCTGTGTTC ACAGGTGAAC GAAGGGGTGC GCGAGAAGGA GAACTCCGAC
    CGCCTCGAGT GGATCCAGGC GCACGTGCAG TGCGAAGGCC TGTCCGAGCA ACTTGTGTTC
    AATTCAGTGA CCAATTGCTT GGGGCCACGC AAGTTTCTGC ACAGTGGGAA ACTCTACAAG
    GCCAAGAGCA ACAAGGAGCT GTATGGCTTC CTCTTCAACG ACTTCCTCCT GCTGACCCAA
    ATCATAAAGC CCTTGGGCTC TTCCGGCACC GACAAAGTCT TCAGTCCCAA ATCTAACCTG
    CAGTATAAAA TGTACAAAAC ACCTATTTTC CTGAATGAGG TTCTAGTAAA ATTACCCACT
    GACCCTTCTG GAGATGAGCC CATCTTCCAC ATCTCCCACA TTGACCGAGT CTACACCCTC
    CGAGCCGAAA GCATAAATGA AAGGACTGCC TGGGTGCAGA AAATCAAAGC TGCTTCAGAA
    CTTTATATAG AGACTGAGAA GAAGAAGCGG GAGAAGGCGT ACCTGGTCCG TTCCCAAAGG
    GCGACAGGTA TTGGAAGGTT GATGGTGAAC GTCGTTGAGG GCATTGAGCT GAAACCCTGT
    CGATCACATG GAAAGAGTAA CCCGTACTGT GAGGTGACCA TGGGTTCCCA GTGTCACATC
    ACCAAGACGA TCCAGGACAC TCTGAATCCC AAGTGGAATT CCAACTGCCA GTTCTTCATC
    AAAGACCTGG AGCAGGAGGT CCTGTGCATC ACCGTGTTTG AGAGGGACCA GTTCTCTCCA
    GACGATTTTT TGGGTCGGAC AGAGATCCGT GTGGCCGACA TCAAGAAGGA CCAGGGTTCC
    AAGGGTCCAG TTACAAAGTG TCTCCTGCTA CACGAAGTTC CCACAGGAGA GATTGTGGTC
    CGCTTGGACC TGCAGCTGTT TGATGAGCCA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe29391
    Clone ID Related Accession (Same CDS sequence) XM_005638880.3 , XM_005638880.2
    Accession Version XM_005638880.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5166bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product intersectin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005638880.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGCTCAGT TTCCAACACC TTTTGGAGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAA 
    GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG
    ATATGGGCGC TAGCTGACAT GAACAATGAT GGACGAATGG ATCAAGTGGA GTTTTCCATA
    GCTATGAAAC TCATCAAACT CAAGCTGCAA GGATATCAAC TTCCTTCTGC ACTTCCCCCC
    GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CCTTTGGTAT AGGAGGTATT
    GCCAGCATGC CACCACTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTC
    GGAATGTCTC CACCTCTAGT ATCTTCTGTT CCTACAGCAG CAGTGCCTCC CCTGGCTAAC
    GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA
    AAGAGCTCTT CCTTCAGTAG ATCTGGTCCC GGGTCACAAT TAAACACTAA GTTACAGAAG
    GCACAGTCAT TTGATGTGGC TAGTGTCCCT CCCGTGGCAG AATGGGCTGT CCCTCAGTCA
    TCCAGACTTA AATACAGACA GTTATTCAAC AGTCATGACA AAACTATGAG TGGACACTTA
    ACAGGTCCAC AAGCAAGGAC TATTCTTATG CAATCAAGTT TGCCACAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC
    TTGGCTATGC ACCTAATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT
    CCAGAGTACA TTCCACCTTC CTTTAGACGA GTTCGATCTG GCAGTGGTAT ATCAGTCATC
    AGCTCAACAG CAGTAGATCA GAGGTTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA
    CAACTAGAAA AGAAATTACC TGTAACCTTT GAAGATAAGA AGCGTGAGAA TTTTGAACGT
    GGCAATCTGG AACTCGAGAA ACGGAGACAA GCGCTCTTGG AGCAGCAGCG CAAAGAGCAA
    GAGCGCCTGG CACAGCTTGA ACGTGCAGAG CAAGAACGGA AAGAGCGGGA GCGCCAGGAG
    CAAGAGCGCA AAAGGCAACT GGAGTTGGAG AAACAACTGG AAAAGCAACG GGAGCTAGAA
    CGTCAGAGAG AAGAGGAGAG GAGGAAAGAA ATTGAGAGAC GAGAGGCTGC AAAGAGAGAA
    CTTGAAAGAC AGCGGCAACT GGAATGGGAA CGAAATCGAA GACAAGAGCT ACTCAATCAG
    AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAGAAGAC TCTAGAATTT
    GAATTAGAAG CTCTTAATGA TAAAAAGCAT CAACTAGAAG GAAAACTTCA AGATATCAGG
    TGTCGATTGA CCACCCAACG GCAAGAAATT GAGAGTACAA ATAAATCCAG AGAGTTGAGA
    ATTGCTGAAA TCACCCATCT ACAGCAACAA TTACAGGAAG CCCAGCAAAT GCTTGGAAGA
    CTTATTCCAG AAAAGCAGAT ACTCAATGAC CAATTAAAAC AAGTTCAACA GAACAGTTTG
    CACAGAGATT CACTGCTTAC ACTCAAAAGA GCGTTAGAAG CAAAAGAACT AGCTCGGCAG
    CAGCTACGCG ATCAACTGGA TGAAGTTGAG AAAGAAACCA GATCAAAACT ACAGGAGATT
    GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ATAGTAAGCA ACAACTCCAG
    AAACAAAAGT CCATAGAGGC CGAACGTCTG AAACAGAAAG AACAAGAGCG AAAAACCATA
    GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAGAGGGA CAAGCAGTGG
    ATTGAGCATG TGCAGCAGGA CGATGAGCAT CAGCGGCCAA GGAAGATCCA TGATGAAGAA
    AAACTCCGAA GGGAAGAGAG TGTCAAAAAG AAGGACAGTG AGGAAAAAAG CAAACTGGAC
    ACGCAAGACA AGATGAGTCG GCTTTTCCAC CAGCATCAAG AGCCAGCCAA GCCAGCTGTC
    CAGGCACCCT GGTCCACCAC AGAAAAAGGC CCACTTACAA TTTCTGCACA GGAGAACGTA
    AAGGTGGTGT ATTACCGAGC GCTCTATCCC TTCGAATCCA GAAGTCATGA TGAGATCACG
    ATCCAGCCTG GAGACATAGT AATGGTTAAA GGGGAATGGG TGGATGAAAG CCAAACTGGA
    GAACCGGGCT GGCTTGGAGG AGAATTAAAA GGAAAGACGG GATGGTTCCC TGCTAACTAC
    GCAGAGAAAA TCCCAGAAAA CGAGGTGCCC GCTCCAGTCA AACCCGGGCC CGACCTCACC
    TCTGCCCCTG CGCCCAAACT GGCCGTGCGT GAGACCCCCG CTCCGGTGGC AGTGAGCGCT
    CCGGAGCCCT CCACGACCCC CAACAGCTGG GCTGACTTCA GCTCCACGTG GCCCACCAGC
    ACGAATGAGA AGCCAGAAAC CGACAACTGG GACGCATGGG CAGCCCAGCC CTCACTCACT
    GTTCCCAGTG CTGGCCAGCT AAGGCAGAGA TCAGCGTTTA CTCCAGCCAC GGCCACTGGC
    TCCTCTCCAT CTCCCGTTTT AGGCCAGGGT GAGAAGGTGG AAGGGCTACA AGCACAAGCC
    CTGTATCCTT GGAGAGCCAA AAAAGACAAC CACTTAAATT TTAACAAAAA TGACGTCATC
    ACCGTCCTGG AACAGCAAGA CATGTGGTGG TTTGGAGAAG TCCAAGGTCA GAAGGGTTGG
    TTCCCCAAGT CTTACGTGAA ACTCATTTCA GGGCCCATAA GGAAATCCAC AAGCATGGAT
    TCTGGTTCTT CAGAAAGTCC CGCTAGTCTA AAGAGAGTAG CTTCCCCAGC AGCCAAGCCG
    GCCATTTCGG GAGAAGAATT TATTGCCATG TACACTTACG AGAGTTCTGA GCAAGGCGAT
    TTAACGTTTC AGCAAGGGGA TGTGATTTTG GTTACCAAGA AAGATGGTGA CTGGTGGACA
    GGAGCAGTGG GCGACAAGTC CGGAGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCA
    GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATTGCGCAG
    GTTATCGCCT GCTACACAGC TACTGGTCCC GAGCAGCTCA CCCTGGCCCC TGGTCAGCTG
    ATTTTGATCC GAAAAAAGAA CCCAGGCGGT TGGTGGGAAG GAGAGCTGCA AGCCCGTGGG
    AAGAAGCGCC AGATAGGCTG GTTCCCCGCG AATTATGTAA AACTTTTGAG CCCTGGCACG
    AGCAAAATCA CTCCAACGGA GCCACCTAAG CCCACGGCGT TCCCGGCAGT GTGCCAGGTG
    ATCGGCGTGT ACGACTACAG CGCCCAGAAC GACGACGAGC TGGCCTTCAG CAAGGGCCAG
    ATCATCAACG TCCTCAACAA AGACGACCCG GACTGGTGGA AGGGCGAGGT CAACGGGCAA
    GTGGGGCTCT TCCCCTCCAA CTACGTGAAG CTGACCACGG ACATGGACCC AAGCCAGCAA
    TGGTGCTCAG ACTTACACCT CTTGGATATG TTGACCCCAA CGGAGAGAAA GCGACAAGGA
    TACATCCATG AGCTCATTGT CACAGAGGAG AACTATGTGA ATGACCTGCA GCTGGTCACA
    GAGATCTTTC AGAAACCCCT GATGGAGTCT GAGCTGCTGA CAGAAAAAGA GGGTGCTATG
    ATTTTTGTTA ACTGGAAGGA GCTGATTATG TGTAATATCA AACTATTGAA AGCGCTGAGG
    GTCCGCAAGA AGATGTCGGG GGAGAAGATG CCCGTGAAGA TGATCGGCGA CATCCTGACG
    GCGCAGCTGC CGCACATGCA GCCTTACATC CGCTTCTGCA GCTGTCAGCT CAACGGGGCT
    GCCCTCATCC AGCAGAAGAC CGACGAGGCC CCGGACTTCA AGGAGTTTGT CAAAAGGCTG
    GCAATGGACC CTCGATGTAA AGGGATGCCA CTCTCTAGTT TTATCCTGAA GCCTATGCAA
    CGCGTAACGA GATACCCACT GATCATTAAA AATATCCTGG AAAACACTCC TGAGAACCAC
    CCCGACCACA GCCACCTGAA GCACGCCCTG GAGAAGGCCG AAGAGCTGTG TTCACAGGTG
    AACGAAGGGG TGCGCGAGAA GGAGAACTCC GACCGCCTCG AGTGGATCCA GGCGCACGTG
    CAGTGCGAAG GCCTGTCCGA GCAACTTGTG TTCAATTCAG TGACCAATTG CTTGGGGCCA
    CGCAAGTTTC TGCACAGTGG GAAACTCTAC AAGGCCAAGA GCAACAAGGA GCTGTATGGC
    TTCCTCTTCA ACGACTTCCT CCTGCTGACC CAAATCATAA AGCCCTTGGG CTCTTCCGGC
    ACCGACAAAG TCTTCAGTCC CAAATCTAAC CTGCAGTATA AAATGTACAA AACACCTATT
    TTCCTGAATG AGGTTCTAGT AAAATTACCC ACTGACCCTT CTGGAGATGA GCCCATCTTC
    CACATCTCCC ACATTGACCG AGTCTACACC CTCCGAGCCG AAAGCATAAA TGAAAGGACT
    GCCTGGGTGC AGAAAATCAA AGCTGCTTCA GAACTTTATA TAGAGACTGA GAAGAAGAAG
    CGGGAGAAGG CGTACCTGGT CCGTTCCCAA AGGGCGACAG GTATTGGAAG GTTGATGGTG
    AACGTCGTTG AGGGCATTGA GCTGAAACCC TGTCGATCAC ATGGAAAGAG TAACCCGTAC
    TGTGAGGTGA CCATGGGTTC CCAGTGTCAC ATCACCAAGA CGATCCAGGA CACTCTGAAT
    CCCAAGTGGA ATTCCAACTG CCAGTTCTTC ATCAAAGACC TGGAGCAGGA GGTCCTGTGC
    ATCACCGTGT TTGAGAGGGA CCAGTTCTCT CCAGACGATT TTTTGGGTCG GACAGAGATC
    CGTGTGGCCG ACATCAAGAA GGACCAGGGT TCCAAGGGTC CAGTTACAAA GTGTCTCCTG
    CTACACGAAG TTCCCACAGG AGAGATTGTG GTCCGCTTGG ACCTGCAGCT GTTTGATGAG
    CCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005638937.1
    CDS256..5421
    Translation

    Target ORF information:

    RefSeq Version XM_005638880.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris intersectin 1 (ITSN1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005638880.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGCTCAGT TTCCAACACC TTTTGGAGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAA 
    GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG
    ATATGGGCGC TAGCTGACAT GAACAATGAT GGACGAATGG ATCAAGTGGA GTTTTCCATA
    GCTATGAAAC TCATCAAACT CAAGCTGCAA GGATATCAAC TTCCTTCTGC ACTTCCCCCC
    GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CCTTTGGTAT AGGAGGTATT
    GCCAGCATGC CACCACTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTC
    GGAATGTCTC CACCTCTAGT ATCTTCTGTT CCTACAGCAG CAGTGCCTCC CCTGGCTAAC
    GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA
    AAGAGCTCTT CCTTCAGTAG ATCTGGTCCC GGGTCACAAT TAAACACTAA GTTACAGAAG
    GCACAGTCAT TTGATGTGGC TAGTGTCCCT CCCGTGGCAG AATGGGCTGT CCCTCAGTCA
    TCCAGACTTA AATACAGACA GTTATTCAAC AGTCATGACA AAACTATGAG TGGACACTTA
    ACAGGTCCAC AAGCAAGGAC TATTCTTATG CAATCAAGTT TGCCACAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC
    TTGGCTATGC ACCTAATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT
    CCAGAGTACA TTCCACCTTC CTTTAGACGA GTTCGATCTG GCAGTGGTAT ATCAGTCATC
    AGCTCAACAG CAGTAGATCA GAGGTTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA
    CAACTAGAAA AGAAATTACC TGTAACCTTT GAAGATAAGA AGCGTGAGAA TTTTGAACGT
    GGCAATCTGG AACTCGAGAA ACGGAGACAA GCGCTCTTGG AGCAGCAGCG CAAAGAGCAA
    GAGCGCCTGG CACAGCTTGA ACGTGCAGAG CAAGAACGGA AAGAGCGGGA GCGCCAGGAG
    CAAGAGCGCA AAAGGCAACT GGAGTTGGAG AAACAACTGG AAAAGCAACG GGAGCTAGAA
    CGTCAGAGAG AAGAGGAGAG GAGGAAAGAA ATTGAGAGAC GAGAGGCTGC AAAGAGAGAA
    CTTGAAAGAC AGCGGCAACT GGAATGGGAA CGAAATCGAA GACAAGAGCT ACTCAATCAG
    AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAGAAGAC TCTAGAATTT
    GAATTAGAAG CTCTTAATGA TAAAAAGCAT CAACTAGAAG GAAAACTTCA AGATATCAGG
    TGTCGATTGA CCACCCAACG GCAAGAAATT GAGAGTACAA ATAAATCCAG AGAGTTGAGA
    ATTGCTGAAA TCACCCATCT ACAGCAACAA TTACAGGAAG CCCAGCAAAT GCTTGGAAGA
    CTTATTCCAG AAAAGCAGAT ACTCAATGAC CAATTAAAAC AAGTTCAACA GAACAGTTTG
    CACAGAGATT CACTGCTTAC ACTCAAAAGA GCGTTAGAAG CAAAAGAACT AGCTCGGCAG
    CAGCTACGCG ATCAACTGGA TGAAGTTGAG AAAGAAACCA GATCAAAACT ACAGGAGATT
    GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ATAGTAAGCA ACAACTCCAG
    AAACAAAAGT CCATAGAGGC CGAACGTCTG AAACAGAAAG AACAAGAGCG AAAAACCATA
    GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAGAGGGA CAAGCAGTGG
    ATTGAGCATG TGCAGCAGGA CGATGAGCAT CAGCGGCCAA GGAAGATCCA TGATGAAGAA
    AAACTCCGAA GGGAAGAGAG TGTCAAAAAG AAGGACAGTG AGGAAAAAAG CAAACTGGAC
    ACGCAAGACA AGATGAGTCG GCTTTTCCAC CAGCATCAAG AGCCAGCCAA GCCAGCTGTC
    CAGGCACCCT GGTCCACCAC AGAAAAAGGC CCACTTACAA TTTCTGCACA GGAGAACGTA
    AAGGTGGTGT ATTACCGAGC GCTCTATCCC TTCGAATCCA GAAGTCATGA TGAGATCACG
    ATCCAGCCTG GAGACATAGT AATGGTTAAA GGGGAATGGG TGGATGAAAG CCAAACTGGA
    GAACCGGGCT GGCTTGGAGG AGAATTAAAA GGAAAGACGG GATGGTTCCC TGCTAACTAC
    GCAGAGAAAA TCCCAGAAAA CGAGGTGCCC GCTCCAGTCA AACCCGGGCC CGACCTCACC
    TCTGCCCCTG CGCCCAAACT GGCCGTGCGT GAGACCCCCG CTCCGGTGGC AGTGAGCGCT
    CCGGAGCCCT CCACGACCCC CAACAGCTGG GCTGACTTCA GCTCCACGTG GCCCACCAGC
    ACGAATGAGA AGCCAGAAAC CGACAACTGG GACGCATGGG CAGCCCAGCC CTCACTCACT
    GTTCCCAGTG CTGGCCAGCT AAGGCAGAGA TCAGCGTTTA CTCCAGCCAC GGCCACTGGC
    TCCTCTCCAT CTCCCGTTTT AGGCCAGGGT GAGAAGGTGG AAGGGCTACA AGCACAAGCC
    CTGTATCCTT GGAGAGCCAA AAAAGACAAC CACTTAAATT TTAACAAAAA TGACGTCATC
    ACCGTCCTGG AACAGCAAGA CATGTGGTGG TTTGGAGAAG TCCAAGGTCA GAAGGGTTGG
    TTCCCCAAGT CTTACGTGAA ACTCATTTCA GGGCCCATAA GGAAATCCAC AAGCATGGAT
    TCTGGTTCTT CAGAAAGTCC CGCTAGTCTA AAGAGAGTAG CTTCCCCAGC AGCCAAGCCG
    GCCATTTCGG GAGAAGAATT TATTGCCATG TACACTTACG AGAGTTCTGA GCAAGGCGAT
    TTAACGTTTC AGCAAGGGGA TGTGATTTTG GTTACCAAGA AAGATGGTGA CTGGTGGACA
    GGAGCAGTGG GCGACAAGTC CGGAGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCA
    GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATTGCGCAG
    GTTATCGCCT GCTACACAGC TACTGGTCCC GAGCAGCTCA CCCTGGCCCC TGGTCAGCTG
    ATTTTGATCC GAAAAAAGAA CCCAGGCGGT TGGTGGGAAG GAGAGCTGCA AGCCCGTGGG
    AAGAAGCGCC AGATAGGCTG GTTCCCCGCG AATTATGTAA AACTTTTGAG CCCTGGCACG
    AGCAAAATCA CTCCAACGGA GCCACCTAAG CCCACGGCGT TCCCGGCAGT GTGCCAGGTG
    ATCGGCGTGT ACGACTACAG CGCCCAGAAC GACGACGAGC TGGCCTTCAG CAAGGGCCAG
    ATCATCAACG TCCTCAACAA AGACGACCCG GACTGGTGGA AGGGCGAGGT CAACGGGCAA
    GTGGGGCTCT TCCCCTCCAA CTACGTGAAG CTGACCACGG ACATGGACCC AAGCCAGCAA
    TGGTGCTCAG ACTTACACCT CTTGGATATG TTGACCCCAA CGGAGAGAAA GCGACAAGGA
    TACATCCATG AGCTCATTGT CACAGAGGAG AACTATGTGA ATGACCTGCA GCTGGTCACA
    GAGATCTTTC AGAAACCCCT GATGGAGTCT GAGCTGCTGA CAGAAAAAGA GGGTGCTATG
    ATTTTTGTTA ACTGGAAGGA GCTGATTATG TGTAATATCA AACTATTGAA AGCGCTGAGG
    GTCCGCAAGA AGATGTCGGG GGAGAAGATG CCCGTGAAGA TGATCGGCGA CATCCTGACG
    GCGCAGCTGC CGCACATGCA GCCTTACATC CGCTTCTGCA GCTGTCAGCT CAACGGGGCT
    GCCCTCATCC AGCAGAAGAC CGACGAGGCC CCGGACTTCA AGGAGTTTGT CAAAAGGCTG
    GCAATGGACC CTCGATGTAA AGGGATGCCA CTCTCTAGTT TTATCCTGAA GCCTATGCAA
    CGCGTAACGA GATACCCACT GATCATTAAA AATATCCTGG AAAACACTCC TGAGAACCAC
    CCCGACCACA GCCACCTGAA GCACGCCCTG GAGAAGGCCG AAGAGCTGTG TTCACAGGTG
    AACGAAGGGG TGCGCGAGAA GGAGAACTCC GACCGCCTCG AGTGGATCCA GGCGCACGTG
    CAGTGCGAAG GCCTGTCCGA GCAACTTGTG TTCAATTCAG TGACCAATTG CTTGGGGCCA
    CGCAAGTTTC TGCACAGTGG GAAACTCTAC AAGGCCAAGA GCAACAAGGA GCTGTATGGC
    TTCCTCTTCA ACGACTTCCT CCTGCTGACC CAAATCATAA AGCCCTTGGG CTCTTCCGGC
    ACCGACAAAG TCTTCAGTCC CAAATCTAAC CTGCAGTATA AAATGTACAA AACACCTATT
    TTCCTGAATG AGGTTCTAGT AAAATTACCC ACTGACCCTT CTGGAGATGA GCCCATCTTC
    CACATCTCCC ACATTGACCG AGTCTACACC CTCCGAGCCG AAAGCATAAA TGAAAGGACT
    GCCTGGGTGC AGAAAATCAA AGCTGCTTCA GAACTTTATA TAGAGACTGA GAAGAAGAAG
    CGGGAGAAGG CGTACCTGGT CCGTTCCCAA AGGGCGACAG GTATTGGAAG GTTGATGGTG
    AACGTCGTTG AGGGCATTGA GCTGAAACCC TGTCGATCAC ATGGAAAGAG TAACCCGTAC
    TGTGAGGTGA CCATGGGTTC CCAGTGTCAC ATCACCAAGA CGATCCAGGA CACTCTGAAT
    CCCAAGTGGA ATTCCAACTG CCAGTTCTTC ATCAAAGACC TGGAGCAGGA GGTCCTGTGC
    ATCACCGTGT TTGAGAGGGA CCAGTTCTCT CCAGACGATT TTTTGGGTCG GACAGAGATC
    CGTGTGGCCG ACATCAAGAA GGACCAGGGT TCCAAGGGTC CAGTTACAAA GTGTCTCCTG
    CTACACGAAG TTCCCACAGG AGAGATTGTG GTCCGCTTGG ACCTGCAGCT GTTTGATGAG
    CCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe29391
    Clone ID Related Accession (Same CDS sequence) XM_005638880.3 , XM_005638880.2
    Accession Version XM_005638880.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5166bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product intersectin-1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005638880.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGCTCAGT TTCCAACACC TTTTGGAGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAA 
    GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG
    ATATGGGCGC TAGCTGACAT GAACAATGAT GGACGAATGG ATCAAGTGGA GTTTTCCATA
    GCTATGAAAC TCATCAAACT CAAGCTGCAA GGATATCAAC TTCCTTCTGC ACTTCCCCCC
    GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CCTTTGGTAT AGGAGGTATT
    GCCAGCATGC CACCACTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTC
    GGAATGTCTC CACCTCTAGT ATCTTCTGTT CCTACAGCAG CAGTGCCTCC CCTGGCTAAC
    GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA
    AAGAGCTCTT CCTTCAGTAG ATCTGGTCCC GGGTCACAAT TAAACACTAA GTTACAGAAG
    GCACAGTCAT TTGATGTGGC TAGTGTCCCT CCCGTGGCAG AATGGGCTGT CCCTCAGTCA
    TCCAGACTTA AATACAGACA GTTATTCAAC AGTCATGACA AAACTATGAG TGGACACTTA
    ACAGGTCCAC AAGCAAGGAC TATTCTTATG CAATCAAGTT TGCCACAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC
    TTGGCTATGC ACCTAATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT
    CCAGAGTACA TTCCACCTTC CTTTAGACGA GTTCGATCTG GCAGTGGTAT ATCAGTCATC
    AGCTCAACAG CAGTAGATCA GAGGTTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA
    CAACTAGAAA AGAAATTACC TGTAACCTTT GAAGATAAGA AGCGTGAGAA TTTTGAACGT
    GGCAATCTGG AACTCGAGAA ACGGAGACAA GCGCTCTTGG AGCAGCAGCG CAAAGAGCAA
    GAGCGCCTGG CACAGCTTGA ACGTGCAGAG CAAGAACGGA AAGAGCGGGA GCGCCAGGAG
    CAAGAGCGCA AAAGGCAACT GGAGTTGGAG AAACAACTGG AAAAGCAACG GGAGCTAGAA
    CGTCAGAGAG AAGAGGAGAG GAGGAAAGAA ATTGAGAGAC GAGAGGCTGC AAAGAGAGAA
    CTTGAAAGAC AGCGGCAACT GGAATGGGAA CGAAATCGAA GACAAGAGCT ACTCAATCAG
    AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAGAAGAC TCTAGAATTT
    GAATTAGAAG CTCTTAATGA TAAAAAGCAT CAACTAGAAG GAAAACTTCA AGATATCAGG
    TGTCGATTGA CCACCCAACG GCAAGAAATT GAGAGTACAA ATAAATCCAG AGAGTTGAGA
    ATTGCTGAAA TCACCCATCT ACAGCAACAA TTACAGGAAG CCCAGCAAAT GCTTGGAAGA
    CTTATTCCAG AAAAGCAGAT ACTCAATGAC CAATTAAAAC AAGTTCAACA GAACAGTTTG
    CACAGAGATT CACTGCTTAC ACTCAAAAGA GCGTTAGAAG CAAAAGAACT AGCTCGGCAG
    CAGCTACGCG ATCAACTGGA TGAAGTTGAG AAAGAAACCA GATCAAAACT ACAGGAGATT
    GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ATAGTAAGCA ACAACTCCAG
    AAACAAAAGT CCATAGAGGC CGAACGTCTG AAACAGAAAG AACAAGAGCG AAAAACCATA
    GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAGAGGGA CAAGCAGTGG
    ATTGAGCATG TGCAGCAGGA CGATGAGCAT CAGCGGCCAA GGAAGATCCA TGATGAAGAA
    AAACTCCGAA GGGAAGAGAG TGTCAAAAAG AAGGACAGTG AGGAAAAAAG CAAACTGGAC
    ACGCAAGACA AGATGAGTCG GCTTTTCCAC CAGCATCAAG AGCCAGCCAA GCCAGCTGTC
    CAGGCACCCT GGTCCACCAC AGAAAAAGGC CCACTTACAA TTTCTGCACA GGAGAACGTA
    AAGGTGGTGT ATTACCGAGC GCTCTATCCC TTCGAATCCA GAAGTCATGA TGAGATCACG
    ATCCAGCCTG GAGACATAGT AATGGTTAAA GGGGAATGGG TGGATGAAAG CCAAACTGGA
    GAACCGGGCT GGCTTGGAGG AGAATTAAAA GGAAAGACGG GATGGTTCCC TGCTAACTAC
    GCAGAGAAAA TCCCAGAAAA CGAGGTGCCC GCTCCAGTCA AACCCGGGCC CGACCTCACC
    TCTGCCCCTG CGCCCAAACT GGCCGTGCGT GAGACCCCCG CTCCGGTGGC AGTGAGCGCT
    CCGGAGCCCT CCACGACCCC CAACAGCTGG GCTGACTTCA GCTCCACGTG GCCCACCAGC
    ACGAATGAGA AGCCAGAAAC CGACAACTGG GACGCATGGG CAGCCCAGCC CTCACTCACT
    GTTCCCAGTG CTGGCCAGCT AAGGCAGAGA TCAGCGTTTA CTCCAGCCAC GGCCACTGGC
    TCCTCTCCAT CTCCCGTTTT AGGCCAGGGT GAGAAGGTGG AAGGGCTACA AGCACAAGCC
    CTGTATCCTT GGAGAGCCAA AAAAGACAAC CACTTAAATT TTAACAAAAA TGACGTCATC
    ACCGTCCTGG AACAGCAAGA CATGTGGTGG TTTGGAGAAG TCCAAGGTCA GAAGGGTTGG
    TTCCCCAAGT CTTACGTGAA ACTCATTTCA GGGCCCATAA GGAAATCCAC AAGCATGGAT
    TCTGGTTCTT CAGAAAGTCC CGCTAGTCTA AAGAGAGTAG CTTCCCCAGC AGCCAAGCCG
    GCCATTTCGG GAGAAGAATT TATTGCCATG TACACTTACG AGAGTTCTGA GCAAGGCGAT
    TTAACGTTTC AGCAAGGGGA TGTGATTTTG GTTACCAAGA AAGATGGTGA CTGGTGGACA
    GGAGCAGTGG GCGACAAGTC CGGAGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCA
    GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATTGCGCAG
    GTTATCGCCT GCTACACAGC TACTGGTCCC GAGCAGCTCA CCCTGGCCCC TGGTCAGCTG
    ATTTTGATCC GAAAAAAGAA CCCAGGCGGT TGGTGGGAAG GAGAGCTGCA AGCCCGTGGG
    AAGAAGCGCC AGATAGGCTG GTTCCCCGCG AATTATGTAA AACTTTTGAG CCCTGGCACG
    AGCAAAATCA CTCCAACGGA GCCACCTAAG CCCACGGCGT TCCCGGCAGT GTGCCAGGTG
    ATCGGCGTGT ACGACTACAG CGCCCAGAAC GACGACGAGC TGGCCTTCAG CAAGGGCCAG
    ATCATCAACG TCCTCAACAA AGACGACCCG GACTGGTGGA AGGGCGAGGT CAACGGGCAA
    GTGGGGCTCT TCCCCTCCAA CTACGTGAAG CTGACCACGG ACATGGACCC AAGCCAGCAA
    TGGTGCTCAG ACTTACACCT CTTGGATATG TTGACCCCAA CGGAGAGAAA GCGACAAGGA
    TACATCCATG AGCTCATTGT CACAGAGGAG AACTATGTGA ATGACCTGCA GCTGGTCACA
    GAGATCTTTC AGAAACCCCT GATGGAGTCT GAGCTGCTGA CAGAAAAAGA GGGTGCTATG
    ATTTTTGTTA ACTGGAAGGA GCTGATTATG TGTAATATCA AACTATTGAA AGCGCTGAGG
    GTCCGCAAGA AGATGTCGGG GGAGAAGATG CCCGTGAAGA TGATCGGCGA CATCCTGACG
    GCGCAGCTGC CGCACATGCA GCCTTACATC CGCTTCTGCA GCTGTCAGCT CAACGGGGCT
    GCCCTCATCC AGCAGAAGAC CGACGAGGCC CCGGACTTCA AGGAGTTTGT CAAAAGGCTG
    GCAATGGACC CTCGATGTAA AGGGATGCCA CTCTCTAGTT TTATCCTGAA GCCTATGCAA
    CGCGTAACGA GATACCCACT GATCATTAAA AATATCCTGG AAAACACTCC TGAGAACCAC
    CCCGACCACA GCCACCTGAA GCACGCCCTG GAGAAGGCCG AAGAGCTGTG TTCACAGGTG
    AACGAAGGGG TGCGCGAGAA GGAGAACTCC GACCGCCTCG AGTGGATCCA GGCGCACGTG
    CAGTGCGAAG GCCTGTCCGA GCAACTTGTG TTCAATTCAG TGACCAATTG CTTGGGGCCA
    CGCAAGTTTC TGCACAGTGG GAAACTCTAC AAGGCCAAGA GCAACAAGGA GCTGTATGGC
    TTCCTCTTCA ACGACTTCCT CCTGCTGACC CAAATCATAA AGCCCTTGGG CTCTTCCGGC
    ACCGACAAAG TCTTCAGTCC CAAATCTAAC CTGCAGTATA AAATGTACAA AACACCTATT
    TTCCTGAATG AGGTTCTAGT AAAATTACCC ACTGACCCTT CTGGAGATGA GCCCATCTTC
    CACATCTCCC ACATTGACCG AGTCTACACC CTCCGAGCCG AAAGCATAAA TGAAAGGACT
    GCCTGGGTGC AGAAAATCAA AGCTGCTTCA GAACTTTATA TAGAGACTGA GAAGAAGAAG
    CGGGAGAAGG CGTACCTGGT CCGTTCCCAA AGGGCGACAG GTATTGGAAG GTTGATGGTG
    AACGTCGTTG AGGGCATTGA GCTGAAACCC TGTCGATCAC ATGGAAAGAG TAACCCGTAC
    TGTGAGGTGA CCATGGGTTC CCAGTGTCAC ATCACCAAGA CGATCCAGGA CACTCTGAAT
    CCCAAGTGGA ATTCCAACTG CCAGTTCTTC ATCAAAGACC TGGAGCAGGA GGTCCTGTGC
    ATCACCGTGT TTGAGAGGGA CCAGTTCTCT CCAGACGATT TTTTGGGTCG GACAGAGATC
    CGTGTGGCCG ACATCAAGAA GGACCAGGGT TCCAAGGGTC CAGTTACAAA GTGTCTCCTG
    CTACACGAAG TTCCCACAGG AGAGATTGTG GTCCGCTTGG ACCTGCAGCT GTTTGATGAG
    CCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005638937.1
    CDS296..5461
    Translation

    Target ORF information:

    RefSeq Version XM_005638880.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005638880.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGCTCAGT TTCCAACACC TTTTGGAGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAA 
    GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG
    ATATGGGCGC TAGCTGACAT GAACAATGAT GGACGAATGG ATCAAGTGGA GTTTTCCATA
    GCTATGAAAC TCATCAAACT CAAGCTGCAA GGATATCAAC TTCCTTCTGC ACTTCCCCCC
    GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CCTTTGGTAT AGGAGGTATT
    GCCAGCATGC CACCACTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTC
    GGAATGTCTC CACCTCTAGT ATCTTCTGTT CCTACAGCAG CAGTGCCTCC CCTGGCTAAC
    GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA
    AAGAGCTCTT CCTTCAGTAG ATCTGGTCCC GGGTCACAAT TAAACACTAA GTTACAGAAG
    GCACAGTCAT TTGATGTGGC TAGTGTCCCT CCCGTGGCAG AATGGGCTGT CCCTCAGTCA
    TCCAGACTTA AATACAGACA GTTATTCAAC AGTCATGACA AAACTATGAG TGGACACTTA
    ACAGGTCCAC AAGCAAGGAC TATTCTTATG CAATCAAGTT TGCCACAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC
    TTGGCTATGC ACCTAATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT
    CCAGAGTACA TTCCACCTTC CTTTAGACGA GTTCGATCTG GCAGTGGTAT ATCAGTCATC
    AGCTCAACAG CAGTAGATCA GAGGTTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA
    CAACTAGAAA AGAAATTACC TGTAACCTTT GAAGATAAGA AGCGTGAGAA TTTTGAACGT
    GGCAATCTGG AACTCGAGAA ACGGAGACAA GCGCTCTTGG AGCAGCAGCG CAAAGAGCAA
    GAGCGCCTGG CACAGCTTGA ACGTGCAGAG CAAGAACGGA AAGAGCGGGA GCGCCAGGAG
    CAAGAGCGCA AAAGGCAACT GGAGTTGGAG AAACAACTGG AAAAGCAACG GGAGCTAGAA
    CGTCAGAGAG AAGAGGAGAG GAGGAAAGAA ATTGAGAGAC GAGAGGCTGC AAAGAGAGAA
    CTTGAAAGAC AGCGGCAACT GGAATGGGAA CGAAATCGAA GACAAGAGCT ACTCAATCAG
    AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAGAAGAC TCTAGAATTT
    GAATTAGAAG CTCTTAATGA TAAAAAGCAT CAACTAGAAG GAAAACTTCA AGATATCAGG
    TGTCGATTGA CCACCCAACG GCAAGAAATT GAGAGTACAA ATAAATCCAG AGAGTTGAGA
    ATTGCTGAAA TCACCCATCT ACAGCAACAA TTACAGGAAG CCCAGCAAAT GCTTGGAAGA
    CTTATTCCAG AAAAGCAGAT ACTCAATGAC CAATTAAAAC AAGTTCAACA GAACAGTTTG
    CACAGAGATT CACTGCTTAC ACTCAAAAGA GCGTTAGAAG CAAAAGAACT AGCTCGGCAG
    CAGCTACGCG ATCAACTGGA TGAAGTTGAG AAAGAAACCA GATCAAAACT ACAGGAGATT
    GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ATAGTAAGCA ACAACTCCAG
    AAACAAAAGT CCATAGAGGC CGAACGTCTG AAACAGAAAG AACAAGAGCG AAAAACCATA
    GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAGAGGGA CAAGCAGTGG
    ATTGAGCATG TGCAGCAGGA CGATGAGCAT CAGCGGCCAA GGAAGATCCA TGATGAAGAA
    AAACTCCGAA GGGAAGAGAG TGTCAAAAAG AAGGACAGTG AGGAAAAAAG CAAACTGGAC
    ACGCAAGACA AGATGAGTCG GCTTTTCCAC CAGCATCAAG AGCCAGCCAA GCCAGCTGTC
    CAGGCACCCT GGTCCACCAC AGAAAAAGGC CCACTTACAA TTTCTGCACA GGAGAACGTA
    AAGGTGGTGT ATTACCGAGC GCTCTATCCC TTCGAATCCA GAAGTCATGA TGAGATCACG
    ATCCAGCCTG GAGACATAGT AATGGTTAAA GGGGAATGGG TGGATGAAAG CCAAACTGGA
    GAACCGGGCT GGCTTGGAGG AGAATTAAAA GGAAAGACGG GATGGTTCCC TGCTAACTAC
    GCAGAGAAAA TCCCAGAAAA CGAGGTGCCC GCTCCAGTCA AACCCGGGCC CGACCTCACC
    TCTGCCCCTG CGCCCAAACT GGCCGTGCGT GAGACCCCCG CTCCGGTGGC AGTGAGCGCT
    CCGGAGCCCT CCACGACCCC CAACAGCTGG GCTGACTTCA GCTCCACGTG GCCCACCAGC
    ACGAATGAGA AGCCAGAAAC CGACAACTGG GACGCATGGG CAGCCCAGCC CTCACTCACT
    GTTCCCAGTG CTGGCCAGCT AAGGCAGAGA TCAGCGTTTA CTCCAGCCAC GGCCACTGGC
    TCCTCTCCAT CTCCCGTTTT AGGCCAGGGT GAGAAGGTGG AAGGGCTACA AGCACAAGCC
    CTGTATCCTT GGAGAGCCAA AAAAGACAAC CACTTAAATT TTAACAAAAA TGACGTCATC
    ACCGTCCTGG AACAGCAAGA CATGTGGTGG TTTGGAGAAG TCCAAGGTCA GAAGGGTTGG
    TTCCCCAAGT CTTACGTGAA ACTCATTTCA GGGCCCATAA GGAAATCCAC AAGCATGGAT
    TCTGGTTCTT CAGAAAGTCC CGCTAGTCTA AAGAGAGTAG CTTCCCCAGC AGCCAAGCCG
    GCCATTTCGG GAGAAGAATT TATTGCCATG TACACTTACG AGAGTTCTGA GCAAGGCGAT
    TTAACGTTTC AGCAAGGGGA TGTGATTTTG GTTACCAAGA AAGATGGTGA CTGGTGGACA
    GGAGCAGTGG GCGACAAGTC CGGAGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCA
    GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATTGCGCAG
    GTTATCGCCT GCTACACAGC TACTGGTCCC GAGCAGCTCA CCCTGGCCCC TGGTCAGCTG
    ATTTTGATCC GAAAAAAGAA CCCAGGCGGT TGGTGGGAAG GAGAGCTGCA AGCCCGTGGG
    AAGAAGCGCC AGATAGGCTG GTTCCCCGCG AATTATGTAA AACTTTTGAG CCCTGGCACG
    AGCAAAATCA CTCCAACGGA GCCACCTAAG CCCACGGCGT TCCCGGCAGT GTGCCAGGTG
    ATCGGCGTGT ACGACTACAG CGCCCAGAAC GACGACGAGC TGGCCTTCAG CAAGGGCCAG
    ATCATCAACG TCCTCAACAA AGACGACCCG GACTGGTGGA AGGGCGAGGT CAACGGGCAA
    GTGGGGCTCT TCCCCTCCAA CTACGTGAAG CTGACCACGG ACATGGACCC AAGCCAGCAA
    TGGTGCTCAG ACTTACACCT CTTGGATATG TTGACCCCAA CGGAGAGAAA GCGACAAGGA
    TACATCCATG AGCTCATTGT CACAGAGGAG AACTATGTGA ATGACCTGCA GCTGGTCACA
    GAGATCTTTC AGAAACCCCT GATGGAGTCT GAGCTGCTGA CAGAAAAAGA GGGTGCTATG
    ATTTTTGTTA ACTGGAAGGA GCTGATTATG TGTAATATCA AACTATTGAA AGCGCTGAGG
    GTCCGCAAGA AGATGTCGGG GGAGAAGATG CCCGTGAAGA TGATCGGCGA CATCCTGACG
    GCGCAGCTGC CGCACATGCA GCCTTACATC CGCTTCTGCA GCTGTCAGCT CAACGGGGCT
    GCCCTCATCC AGCAGAAGAC CGACGAGGCC CCGGACTTCA AGGAGTTTGT CAAAAGGCTG
    GCAATGGACC CTCGATGTAA AGGGATGCCA CTCTCTAGTT TTATCCTGAA GCCTATGCAA
    CGCGTAACGA GATACCCACT GATCATTAAA AATATCCTGG AAAACACTCC TGAGAACCAC
    CCCGACCACA GCCACCTGAA GCACGCCCTG GAGAAGGCCG AAGAGCTGTG TTCACAGGTG
    AACGAAGGGG TGCGCGAGAA GGAGAACTCC GACCGCCTCG AGTGGATCCA GGCGCACGTG
    CAGTGCGAAG GCCTGTCCGA GCAACTTGTG TTCAATTCAG TGACCAATTG CTTGGGGCCA
    CGCAAGTTTC TGCACAGTGG GAAACTCTAC AAGGCCAAGA GCAACAAGGA GCTGTATGGC
    TTCCTCTTCA ACGACTTCCT CCTGCTGACC CAAATCATAA AGCCCTTGGG CTCTTCCGGC
    ACCGACAAAG TCTTCAGTCC CAAATCTAAC CTGCAGTATA AAATGTACAA AACACCTATT
    TTCCTGAATG AGGTTCTAGT AAAATTACCC ACTGACCCTT CTGGAGATGA GCCCATCTTC
    CACATCTCCC ACATTGACCG AGTCTACACC CTCCGAGCCG AAAGCATAAA TGAAAGGACT
    GCCTGGGTGC AGAAAATCAA AGCTGCTTCA GAACTTTATA TAGAGACTGA GAAGAAGAAG
    CGGGAGAAGG CGTACCTGGT CCGTTCCCAA AGGGCGACAG GTATTGGAAG GTTGATGGTG
    AACGTCGTTG AGGGCATTGA GCTGAAACCC TGTCGATCAC ATGGAAAGAG TAACCCGTAC
    TGTGAGGTGA CCATGGGTTC CCAGTGTCAC ATCACCAAGA CGATCCAGGA CACTCTGAAT
    CCCAAGTGGA ATTCCAACTG CCAGTTCTTC ATCAAAGACC TGGAGCAGGA GGTCCTGTGC
    ATCACCGTGT TTGAGAGGGA CCAGTTCTCT CCAGACGATT TTTTGGGTCG GACAGAGATC
    CGTGTGGCCG ACATCAAGAA GGACCAGGGT TCCAAGGGTC CAGTTACAAA GTGTCTCCTG
    CTACACGAAG TTCCCACAGG AGAGATTGTG GTCCGCTTGG ACCTGCAGCT GTTTGATGAG
    CCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe29392
    Clone ID Related Accession (Same CDS sequence) XM_014109747.2 , XM_014109747.1
    Accession Version XM_014109747.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5151bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product intersectin-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109747.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGCTCAGT TTCCAACACC TTTTGGAGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAA 
    GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG
    ATATGGGCGC TAGCTGACAT GAACAATGAT GGACGAATGG ATCAAGTGGA GTTTTCCATA
    GCTATGAAAC TCATCAAACT CAAGCTGCAA GGATATCAAC TTCCTTCTGC ACTTCCCCCC
    GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CCTTTGGTAT AGGAGGTATT
    GCCAGCATGC CACCACTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTC
    GGAATGTCTC CACCTCTAGT ATCTTCTGTT CCTACAGCAG CAGTGCCTCC CCTGGCTAAC
    GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA
    AAGAGCTCTT CCTTCAGTAG ATCTGGTCCC GGGTCACAAT TAAACACTAA GTTACAGAAG
    GCACAGTCAT TTGATGTGGC TAGTGTCCCT CCCGTGGCAG AATGGGCTGT CCCTCAGTCA
    TCCAGACTTA AATACAGACA GTTATTCAAC AGTCATGACA AAACTATGAG TGGACACTTA
    ACAGGTCCAC AAGCAAGGAC TATTCTTATG CAATCAAGTT TGCCACAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC
    TTGGCTATGC ACCTAATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT
    CCAGAGTACA TTCCACCTTC CTTTAGACGA GTTCGATCTG GCAGTGGTAT ATCAGTCATC
    AGCTCAACAG CAGTAGATCA GAGGTTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA
    CAACTAGAAA AGAAATTACC TGTAACCTTT GAAGATAAGA AGCGTGAGAA TTTTGAACGT
    GGCAATCTGG AACTCGAGAA ACGGAGACAA GCGCTCTTGG AGCAGCAGCG CAAAGAGCAA
    GAGCGCCTGG CACAGCTTGA ACGTGCAGAG CAAGAACGGA AAGAGCGGGA GCGCCAGGAG
    CAAGAGCGCA AAAGGCAACT GGAGTTGGAG AAACAACTGG AAAAGCAACG GGAGCTAGAA
    CGTCAGAGAG AAGAGGAGAG GAGGAAAGAA ATTGAGAGAC GAGAGGCTGC AAAGAGAGAA
    CTTGAAAGAC AGCGGCAACT GGAATGGGAA CGAAATCGAA GACAAGAGCT ACTCAATCAG
    AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAGAAGAC TCTAGAATTT
    GAATTAGAAG CTCTTAATGA TAAAAAGCAT CAACTAGAAG GAAAACTTCA AGATATCAGG
    TGTCGATTGA CCACCCAACG GCAAGAAATT GAGAGTACAA ATAAATCCAG AGAGTTGAGA
    ATTGCTGAAA TCACCCATCT ACAGCAACAA TTACAGGAAG CCCAGCAAAT GCTTGGAAGA
    CTTATTCCAG AAAAGCAGAT ACTCAATGAC CAATTAAAAC AAGTTCAACA GAACAGTTTG
    CACAGAGATT CACTGCTTAC ACTCAAAAGA GCGTTAGAAG CAAAAGAACT AGCTCGGCAG
    CAGCTACGCG ATCAACTGGA TGAAGTTGAG AAAGAAACCA GATCAAAACT ACAGGAGATT
    GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ATAGTAAGCA ACAACTCCAG
    AAACAAAAGT CCATAGAGGC CGAACGTCTG AAACAGAAAG AACAAGAGCG AAAAACCATA
    GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAGAGGGA CAAGCAGTGG
    ATTGAGCATG TGCAGCAGGA CGATGAGCAT CAGCGGCCAA GGAAGATCCA TGATGAAGAA
    AAACTCCGAA GGGAAGAGAG TGTCAAAAAG AAGGACAGTG AGGAAAAAAG CAAACTGGAC
    ACGCAAGACA AGATGAGTCG GCTTTTCCAC CAGCATCAAG AGCCAGCCAA GCCAGCTGTC
    CAGGCACCCT GGTCCACCAC AGAAAAAGGC CCACTTACAA TTTCTGCACA GGAGAACGTA
    AAGGTGGTGT ATTACCGAGC GCTCTATCCC TTCGAATCCA GAAGTCATGA TGAGATCACG
    ATCCAGCCTG GAGACATAGT AATGGTGGAT GAAAGCCAAA CTGGAGAACC GGGCTGGCTT
    GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCTA ACTACGCAGA GAAAATCCCA
    GAAAACGAGG TGCCCGCTCC AGTCAAACCC GGGCCCGACC TCACCTCTGC CCCTGCGCCC
    AAACTGGCCG TGCGTGAGAC CCCCGCTCCG GTGGCAGTGA GCGCTCCGGA GCCCTCCACG
    ACCCCCAACA GCTGGGCTGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAGCCA
    GAAACCGACA ACTGGGACGC ATGGGCAGCC CAGCCCTCAC TCACTGTTCC CAGTGCTGGC
    CAGCTAAGGC AGAGATCAGC GTTTACTCCA GCCACGGCCA CTGGCTCCTC TCCATCTCCC
    GTTTTAGGCC AGGGTGAGAA GGTGGAAGGG CTACAAGCAC AAGCCCTGTA TCCTTGGAGA
    GCCAAAAAAG ACAACCACTT AAATTTTAAC AAAAATGACG TCATCACCGT CCTGGAACAG
    CAAGACATGT GGTGGTTTGG AGAAGTCCAA GGTCAGAAGG GTTGGTTCCC CAAGTCTTAC
    GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCCACAAGCA TGGATTCTGG TTCTTCAGAA
    AGTCCCGCTA GTCTAAAGAG AGTAGCTTCC CCAGCAGCCA AGCCGGCCAT TTCGGGAGAA
    GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GCGATTTAAC GTTTCAGCAA
    GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAGC AGTGGGCGAC
    AAGTCCGGAG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCAGAGGG CTCTGGAACT
    GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATTG CGCAGGTTAT CGCCTGCTAC
    ACAGCTACTG GTCCCGAGCA GCTCACCCTG GCCCCTGGTC AGCTGATTTT GATCCGAAAA
    AAGAACCCAG GCGGTTGGTG GGAAGGAGAG CTGCAAGCCC GTGGGAAGAA GCGCCAGATA
    GGCTGGTTCC CCGCGAATTA TGTAAAACTT TTGAGCCCTG GCACGAGCAA AATCACTCCA
    ACGGAGCCAC CTAAGCCCAC GGCGTTCCCG GCAGTGTGCC AGGTGATCGG CGTGTACGAC
    TACAGCGCCC AGAACGACGA CGAGCTGGCC TTCAGCAAGG GCCAGATCAT CAACGTCCTC
    AACAAAGACG ACCCGGACTG GTGGAAGGGC GAGGTCAACG GGCAAGTGGG GCTCTTCCCC
    TCCAACTACG TGAAGCTGAC CACGGACATG GACCCAAGCC AGCAATGGTG CTCAGACTTA
    CACCTCTTGG ATATGTTGAC CCCAACGGAG AGAAAGCGAC AAGGATACAT CCATGAGCTC
    ATTGTCACAG AGGAGAACTA TGTGAATGAC CTGCAGCTGG TCACAGAGAT CTTTCAGAAA
    CCCCTGATGG AGTCTGAGCT GCTGACAGAA AAAGAGGGTG CTATGATTTT TGTTAACTGG
    AAGGAGCTGA TTATGTGTAA TATCAAACTA TTGAAAGCGC TGAGGGTCCG CAAGAAGATG
    TCGGGGGAGA AGATGCCCGT GAAGATGATC GGCGACATCC TGACGGCGCA GCTGCCGCAC
    ATGCAGCCTT ACATCCGCTT CTGCAGCTGT CAGCTCAACG GGGCTGCCCT CATCCAGCAG
    AAGACCGACG AGGCCCCGGA CTTCAAGGAG TTTGTCAAAA GGCTGGCAAT GGACCCTCGA
    TGTAAAGGGA TGCCACTCTC TAGTTTTATC CTGAAGCCTA TGCAACGCGT AACGAGATAC
    CCACTGATCA TTAAAAATAT CCTGGAAAAC ACTCCTGAGA ACCACCCCGA CCACAGCCAC
    CTGAAGCACG CCCTGGAGAA GGCCGAAGAG CTGTGTTCAC AGGTGAACGA AGGGGTGCGC
    GAGAAGGAGA ACTCCGACCG CCTCGAGTGG ATCCAGGCGC ACGTGCAGTG CGAAGGCCTG
    TCCGAGCAAC TTGTGTTCAA TTCAGTGACC AATTGCTTGG GGCCACGCAA GTTTCTGCAC
    AGTGGGAAAC TCTACAAGGC CAAGAGCAAC AAGGAGCTGT ATGGCTTCCT CTTCAACGAC
    TTCCTCCTGC TGACCCAAAT CATAAAGCCC TTGGGCTCTT CCGGCACCGA CAAAGTCTTC
    AGTCCCAAAT CTAACCTGCA GTATAAAATG TACAAAACAC CTATTTTCCT GAATGAGGTT
    CTAGTAAAAT TACCCACTGA CCCTTCTGGA GATGAGCCCA TCTTCCACAT CTCCCACATT
    GACCGAGTCT ACACCCTCCG AGCCGAAAGC ATAAATGAAA GGACTGCCTG GGTGCAGAAA
    ATCAAAGCTG CTTCAGAACT TTATATAGAG ACTGAGAAGA AGAAGCGGGA GAAGGCGTAC
    CTGGTCCGTT CCCAAAGGGC GACAGGTATT GGAAGGTTGA TGGTGAACGT CGTTGAGGGC
    ATTGAGCTGA AACCCTGTCG ATCACATGGA AAGAGTAACC CGTACTGTGA GGTGACCATG
    GGTTCCCAGT GTCACATCAC CAAGACGATC CAGGACACTC TGAATCCCAA GTGGAATTCC
    AACTGCCAGT TCTTCATCAA AGACCTGGAG CAGGAGGTCC TGTGCATCAC CGTGTTTGAG
    AGGGACCAGT TCTCTCCAGA CGATTTTTTG GGTCGGACAG AGATCCGTGT GGCCGACATC
    AAGAAGGACC AGGGTTCCAA GGGTCCAGTT ACAAAGTGTC TCCTGCTACA CGAAGTTCCC
    ACAGGAGAGA TTGTGGTCCG CTTGGACCTG CAGCTGTTTG ATGAGCCATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965222.1
    CDS255..5405
    Translation

    Target ORF information:

    RefSeq Version XM_014109747.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris intersectin 1 (ITSN1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109747.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGCTCAGT TTCCAACACC TTTTGGAGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAA 
    GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG
    ATATGGGCGC TAGCTGACAT GAACAATGAT GGACGAATGG ATCAAGTGGA GTTTTCCATA
    GCTATGAAAC TCATCAAACT CAAGCTGCAA GGATATCAAC TTCCTTCTGC ACTTCCCCCC
    GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CCTTTGGTAT AGGAGGTATT
    GCCAGCATGC CACCACTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTC
    GGAATGTCTC CACCTCTAGT ATCTTCTGTT CCTACAGCAG CAGTGCCTCC CCTGGCTAAC
    GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA
    AAGAGCTCTT CCTTCAGTAG ATCTGGTCCC GGGTCACAAT TAAACACTAA GTTACAGAAG
    GCACAGTCAT TTGATGTGGC TAGTGTCCCT CCCGTGGCAG AATGGGCTGT CCCTCAGTCA
    TCCAGACTTA AATACAGACA GTTATTCAAC AGTCATGACA AAACTATGAG TGGACACTTA
    ACAGGTCCAC AAGCAAGGAC TATTCTTATG CAATCAAGTT TGCCACAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC
    TTGGCTATGC ACCTAATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT
    CCAGAGTACA TTCCACCTTC CTTTAGACGA GTTCGATCTG GCAGTGGTAT ATCAGTCATC
    AGCTCAACAG CAGTAGATCA GAGGTTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA
    CAACTAGAAA AGAAATTACC TGTAACCTTT GAAGATAAGA AGCGTGAGAA TTTTGAACGT
    GGCAATCTGG AACTCGAGAA ACGGAGACAA GCGCTCTTGG AGCAGCAGCG CAAAGAGCAA
    GAGCGCCTGG CACAGCTTGA ACGTGCAGAG CAAGAACGGA AAGAGCGGGA GCGCCAGGAG
    CAAGAGCGCA AAAGGCAACT GGAGTTGGAG AAACAACTGG AAAAGCAACG GGAGCTAGAA
    CGTCAGAGAG AAGAGGAGAG GAGGAAAGAA ATTGAGAGAC GAGAGGCTGC AAAGAGAGAA
    CTTGAAAGAC AGCGGCAACT GGAATGGGAA CGAAATCGAA GACAAGAGCT ACTCAATCAG
    AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAGAAGAC TCTAGAATTT
    GAATTAGAAG CTCTTAATGA TAAAAAGCAT CAACTAGAAG GAAAACTTCA AGATATCAGG
    TGTCGATTGA CCACCCAACG GCAAGAAATT GAGAGTACAA ATAAATCCAG AGAGTTGAGA
    ATTGCTGAAA TCACCCATCT ACAGCAACAA TTACAGGAAG CCCAGCAAAT GCTTGGAAGA
    CTTATTCCAG AAAAGCAGAT ACTCAATGAC CAATTAAAAC AAGTTCAACA GAACAGTTTG
    CACAGAGATT CACTGCTTAC ACTCAAAAGA GCGTTAGAAG CAAAAGAACT AGCTCGGCAG
    CAGCTACGCG ATCAACTGGA TGAAGTTGAG AAAGAAACCA GATCAAAACT ACAGGAGATT
    GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ATAGTAAGCA ACAACTCCAG
    AAACAAAAGT CCATAGAGGC CGAACGTCTG AAACAGAAAG AACAAGAGCG AAAAACCATA
    GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAGAGGGA CAAGCAGTGG
    ATTGAGCATG TGCAGCAGGA CGATGAGCAT CAGCGGCCAA GGAAGATCCA TGATGAAGAA
    AAACTCCGAA GGGAAGAGAG TGTCAAAAAG AAGGACAGTG AGGAAAAAAG CAAACTGGAC
    ACGCAAGACA AGATGAGTCG GCTTTTCCAC CAGCATCAAG AGCCAGCCAA GCCAGCTGTC
    CAGGCACCCT GGTCCACCAC AGAAAAAGGC CCACTTACAA TTTCTGCACA GGAGAACGTA
    AAGGTGGTGT ATTACCGAGC GCTCTATCCC TTCGAATCCA GAAGTCATGA TGAGATCACG
    ATCCAGCCTG GAGACATAGT AATGGTGGAT GAAAGCCAAA CTGGAGAACC GGGCTGGCTT
    GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCTA ACTACGCAGA GAAAATCCCA
    GAAAACGAGG TGCCCGCTCC AGTCAAACCC GGGCCCGACC TCACCTCTGC CCCTGCGCCC
    AAACTGGCCG TGCGTGAGAC CCCCGCTCCG GTGGCAGTGA GCGCTCCGGA GCCCTCCACG
    ACCCCCAACA GCTGGGCTGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAGCCA
    GAAACCGACA ACTGGGACGC ATGGGCAGCC CAGCCCTCAC TCACTGTTCC CAGTGCTGGC
    CAGCTAAGGC AGAGATCAGC GTTTACTCCA GCCACGGCCA CTGGCTCCTC TCCATCTCCC
    GTTTTAGGCC AGGGTGAGAA GGTGGAAGGG CTACAAGCAC AAGCCCTGTA TCCTTGGAGA
    GCCAAAAAAG ACAACCACTT AAATTTTAAC AAAAATGACG TCATCACCGT CCTGGAACAG
    CAAGACATGT GGTGGTTTGG AGAAGTCCAA GGTCAGAAGG GTTGGTTCCC CAAGTCTTAC
    GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCCACAAGCA TGGATTCTGG TTCTTCAGAA
    AGTCCCGCTA GTCTAAAGAG AGTAGCTTCC CCAGCAGCCA AGCCGGCCAT TTCGGGAGAA
    GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GCGATTTAAC GTTTCAGCAA
    GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAGC AGTGGGCGAC
    AAGTCCGGAG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCAGAGGG CTCTGGAACT
    GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATTG CGCAGGTTAT CGCCTGCTAC
    ACAGCTACTG GTCCCGAGCA GCTCACCCTG GCCCCTGGTC AGCTGATTTT GATCCGAAAA
    AAGAACCCAG GCGGTTGGTG GGAAGGAGAG CTGCAAGCCC GTGGGAAGAA GCGCCAGATA
    GGCTGGTTCC CCGCGAATTA TGTAAAACTT TTGAGCCCTG GCACGAGCAA AATCACTCCA
    ACGGAGCCAC CTAAGCCCAC GGCGTTCCCG GCAGTGTGCC AGGTGATCGG CGTGTACGAC
    TACAGCGCCC AGAACGACGA CGAGCTGGCC TTCAGCAAGG GCCAGATCAT CAACGTCCTC
    AACAAAGACG ACCCGGACTG GTGGAAGGGC GAGGTCAACG GGCAAGTGGG GCTCTTCCCC
    TCCAACTACG TGAAGCTGAC CACGGACATG GACCCAAGCC AGCAATGGTG CTCAGACTTA
    CACCTCTTGG ATATGTTGAC CCCAACGGAG AGAAAGCGAC AAGGATACAT CCATGAGCTC
    ATTGTCACAG AGGAGAACTA TGTGAATGAC CTGCAGCTGG TCACAGAGAT CTTTCAGAAA
    CCCCTGATGG AGTCTGAGCT GCTGACAGAA AAAGAGGGTG CTATGATTTT TGTTAACTGG
    AAGGAGCTGA TTATGTGTAA TATCAAACTA TTGAAAGCGC TGAGGGTCCG CAAGAAGATG
    TCGGGGGAGA AGATGCCCGT GAAGATGATC GGCGACATCC TGACGGCGCA GCTGCCGCAC
    ATGCAGCCTT ACATCCGCTT CTGCAGCTGT CAGCTCAACG GGGCTGCCCT CATCCAGCAG
    AAGACCGACG AGGCCCCGGA CTTCAAGGAG TTTGTCAAAA GGCTGGCAAT GGACCCTCGA
    TGTAAAGGGA TGCCACTCTC TAGTTTTATC CTGAAGCCTA TGCAACGCGT AACGAGATAC
    CCACTGATCA TTAAAAATAT CCTGGAAAAC ACTCCTGAGA ACCACCCCGA CCACAGCCAC
    CTGAAGCACG CCCTGGAGAA GGCCGAAGAG CTGTGTTCAC AGGTGAACGA AGGGGTGCGC
    GAGAAGGAGA ACTCCGACCG CCTCGAGTGG ATCCAGGCGC ACGTGCAGTG CGAAGGCCTG
    TCCGAGCAAC TTGTGTTCAA TTCAGTGACC AATTGCTTGG GGCCACGCAA GTTTCTGCAC
    AGTGGGAAAC TCTACAAGGC CAAGAGCAAC AAGGAGCTGT ATGGCTTCCT CTTCAACGAC
    TTCCTCCTGC TGACCCAAAT CATAAAGCCC TTGGGCTCTT CCGGCACCGA CAAAGTCTTC
    AGTCCCAAAT CTAACCTGCA GTATAAAATG TACAAAACAC CTATTTTCCT GAATGAGGTT
    CTAGTAAAAT TACCCACTGA CCCTTCTGGA GATGAGCCCA TCTTCCACAT CTCCCACATT
    GACCGAGTCT ACACCCTCCG AGCCGAAAGC ATAAATGAAA GGACTGCCTG GGTGCAGAAA
    ATCAAAGCTG CTTCAGAACT TTATATAGAG ACTGAGAAGA AGAAGCGGGA GAAGGCGTAC
    CTGGTCCGTT CCCAAAGGGC GACAGGTATT GGAAGGTTGA TGGTGAACGT CGTTGAGGGC
    ATTGAGCTGA AACCCTGTCG ATCACATGGA AAGAGTAACC CGTACTGTGA GGTGACCATG
    GGTTCCCAGT GTCACATCAC CAAGACGATC CAGGACACTC TGAATCCCAA GTGGAATTCC
    AACTGCCAGT TCTTCATCAA AGACCTGGAG CAGGAGGTCC TGTGCATCAC CGTGTTTGAG
    AGGGACCAGT TCTCTCCAGA CGATTTTTTG GGTCGGACAG AGATCCGTGT GGCCGACATC
    AAGAAGGACC AGGGTTCCAA GGGTCCAGTT ACAAAGTGTC TCCTGCTACA CGAAGTTCCC
    ACAGGAGAGA TTGTGGTCCG CTTGGACCTG CAGCTGTTTG ATGAGCCATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe29392
    Clone ID Related Accession (Same CDS sequence) XM_014109747.2 , XM_014109747.1
    Accession Version XM_014109747.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5151bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product intersectin-1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGCTCAGT TTCCAACACC TTTTGGAGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAA 
    GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG
    ATATGGGCGC TAGCTGACAT GAACAATGAT GGACGAATGG ATCAAGTGGA GTTTTCCATA
    GCTATGAAAC TCATCAAACT CAAGCTGCAA GGATATCAAC TTCCTTCTGC ACTTCCCCCC
    GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CCTTTGGTAT AGGAGGTATT
    GCCAGCATGC CACCACTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTC
    GGAATGTCTC CACCTCTAGT ATCTTCTGTT CCTACAGCAG CAGTGCCTCC CCTGGCTAAC
    GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA
    AAGAGCTCTT CCTTCAGTAG ATCTGGTCCC GGGTCACAAT TAAACACTAA GTTACAGAAG
    GCACAGTCAT TTGATGTGGC TAGTGTCCCT CCCGTGGCAG AATGGGCTGT CCCTCAGTCA
    TCCAGACTTA AATACAGACA GTTATTCAAC AGTCATGACA AAACTATGAG TGGACACTTA
    ACAGGTCCAC AAGCAAGGAC TATTCTTATG CAATCAAGTT TGCCACAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC
    TTGGCTATGC ACCTAATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT
    CCAGAGTACA TTCCACCTTC CTTTAGACGA GTTCGATCTG GCAGTGGTAT ATCAGTCATC
    AGCTCAACAG CAGTAGATCA GAGGTTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA
    CAACTAGAAA AGAAATTACC TGTAACCTTT GAAGATAAGA AGCGTGAGAA TTTTGAACGT
    GGCAATCTGG AACTCGAGAA ACGGAGACAA GCGCTCTTGG AGCAGCAGCG CAAAGAGCAA
    GAGCGCCTGG CACAGCTTGA ACGTGCAGAG CAAGAACGGA AAGAGCGGGA GCGCCAGGAG
    CAAGAGCGCA AAAGGCAACT GGAGTTGGAG AAACAACTGG AAAAGCAACG GGAGCTAGAA
    CGTCAGAGAG AAGAGGAGAG GAGGAAAGAA ATTGAGAGAC GAGAGGCTGC AAAGAGAGAA
    CTTGAAAGAC AGCGGCAACT GGAATGGGAA CGAAATCGAA GACAAGAGCT ACTCAATCAG
    AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAGAAGAC TCTAGAATTT
    GAATTAGAAG CTCTTAATGA TAAAAAGCAT CAACTAGAAG GAAAACTTCA AGATATCAGG
    TGTCGATTGA CCACCCAACG GCAAGAAATT GAGAGTACAA ATAAATCCAG AGAGTTGAGA
    ATTGCTGAAA TCACCCATCT ACAGCAACAA TTACAGGAAG CCCAGCAAAT GCTTGGAAGA
    CTTATTCCAG AAAAGCAGAT ACTCAATGAC CAATTAAAAC AAGTTCAACA GAACAGTTTG
    CACAGAGATT CACTGCTTAC ACTCAAAAGA GCGTTAGAAG CAAAAGAACT AGCTCGGCAG
    CAGCTACGCG ATCAACTGGA TGAAGTTGAG AAAGAAACCA GATCAAAACT ACAGGAGATT
    GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ATAGTAAGCA ACAACTCCAG
    AAACAAAAGT CCATAGAGGC CGAACGTCTG AAACAGAAAG AACAAGAGCG AAAAACCATA
    GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAGAGGGA CAAGCAGTGG
    ATTGAGCATG TGCAGCAGGA CGATGAGCAT CAGCGGCCAA GGAAGATCCA TGATGAAGAA
    AAACTCCGAA GGGAAGAGAG TGTCAAAAAG AAGGACAGTG AGGAAAAAAG CAAACTGGAC
    ACGCAAGACA AGATGAGTCG GCTTTTCCAC CAGCATCAAG AGCCAGCCAA GCCAGCTGTC
    CAGGCACCCT GGTCCACCAC AGAAAAAGGC CCACTTACAA TTTCTGCACA GGAGAACGTA
    AAGGTGGTGT ATTACCGAGC GCTCTATCCC TTCGAATCCA GAAGTCATGA TGAGATCACG
    ATCCAGCCTG GAGACATAGT AATGGTGGAT GAAAGCCAAA CTGGAGAACC GGGCTGGCTT
    GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCTA ACTACGCAGA GAAAATCCCA
    GAAAACGAGG TGCCCGCTCC AGTCAAACCC GGGCCCGACC TCACCTCTGC CCCTGCGCCC
    AAACTGGCCG TGCGTGAGAC CCCCGCTCCG GTGGCAGTGA GCGCTCCGGA GCCCTCCACG
    ACCCCCAACA GCTGGGCTGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAGCCA
    GAAACCGACA ACTGGGACGC ATGGGCAGCC CAGCCCTCAC TCACTGTTCC CAGTGCTGGC
    CAGCTAAGGC AGAGATCAGC GTTTACTCCA GCCACGGCCA CTGGCTCCTC TCCATCTCCC
    GTTTTAGGCC AGGGTGAGAA GGTGGAAGGG CTACAAGCAC AAGCCCTGTA TCCTTGGAGA
    GCCAAAAAAG ACAACCACTT AAATTTTAAC AAAAATGACG TCATCACCGT CCTGGAACAG
    CAAGACATGT GGTGGTTTGG AGAAGTCCAA GGTCAGAAGG GTTGGTTCCC CAAGTCTTAC
    GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCCACAAGCA TGGATTCTGG TTCTTCAGAA
    AGTCCCGCTA GTCTAAAGAG AGTAGCTTCC CCAGCAGCCA AGCCGGCCAT TTCGGGAGAA
    GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GCGATTTAAC GTTTCAGCAA
    GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAGC AGTGGGCGAC
    AAGTCCGGAG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCAGAGGG CTCTGGAACT
    GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATTG CGCAGGTTAT CGCCTGCTAC
    ACAGCTACTG GTCCCGAGCA GCTCACCCTG GCCCCTGGTC AGCTGATTTT GATCCGAAAA
    AAGAACCCAG GCGGTTGGTG GGAAGGAGAG CTGCAAGCCC GTGGGAAGAA GCGCCAGATA
    GGCTGGTTCC CCGCGAATTA TGTAAAACTT TTGAGCCCTG GCACGAGCAA AATCACTCCA
    ACGGAGCCAC CTAAGCCCAC GGCGTTCCCG GCAGTGTGCC AGGTGATCGG CGTGTACGAC
    TACAGCGCCC AGAACGACGA CGAGCTGGCC TTCAGCAAGG GCCAGATCAT CAACGTCCTC
    AACAAAGACG ACCCGGACTG GTGGAAGGGC GAGGTCAACG GGCAAGTGGG GCTCTTCCCC
    TCCAACTACG TGAAGCTGAC CACGGACATG GACCCAAGCC AGCAATGGTG CTCAGACTTA
    CACCTCTTGG ATATGTTGAC CCCAACGGAG AGAAAGCGAC AAGGATACAT CCATGAGCTC
    ATTGTCACAG AGGAGAACTA TGTGAATGAC CTGCAGCTGG TCACAGAGAT CTTTCAGAAA
    CCCCTGATGG AGTCTGAGCT GCTGACAGAA AAAGAGGGTG CTATGATTTT TGTTAACTGG
    AAGGAGCTGA TTATGTGTAA TATCAAACTA TTGAAAGCGC TGAGGGTCCG CAAGAAGATG
    TCGGGGGAGA AGATGCCCGT GAAGATGATC GGCGACATCC TGACGGCGCA GCTGCCGCAC
    ATGCAGCCTT ACATCCGCTT CTGCAGCTGT CAGCTCAACG GGGCTGCCCT CATCCAGCAG
    AAGACCGACG AGGCCCCGGA CTTCAAGGAG TTTGTCAAAA GGCTGGCAAT GGACCCTCGA
    TGTAAAGGGA TGCCACTCTC TAGTTTTATC CTGAAGCCTA TGCAACGCGT AACGAGATAC
    CCACTGATCA TTAAAAATAT CCTGGAAAAC ACTCCTGAGA ACCACCCCGA CCACAGCCAC
    CTGAAGCACG CCCTGGAGAA GGCCGAAGAG CTGTGTTCAC AGGTGAACGA AGGGGTGCGC
    GAGAAGGAGA ACTCCGACCG CCTCGAGTGG ATCCAGGCGC ACGTGCAGTG CGAAGGCCTG
    TCCGAGCAAC TTGTGTTCAA TTCAGTGACC AATTGCTTGG GGCCACGCAA GTTTCTGCAC
    AGTGGGAAAC TCTACAAGGC CAAGAGCAAC AAGGAGCTGT ATGGCTTCCT CTTCAACGAC
    TTCCTCCTGC TGACCCAAAT CATAAAGCCC TTGGGCTCTT CCGGCACCGA CAAAGTCTTC
    AGTCCCAAAT CTAACCTGCA GTATAAAATG TACAAAACAC CTATTTTCCT GAATGAGGTT
    CTAGTAAAAT TACCCACTGA CCCTTCTGGA GATGAGCCCA TCTTCCACAT CTCCCACATT
    GACCGAGTCT ACACCCTCCG AGCCGAAAGC ATAAATGAAA GGACTGCCTG GGTGCAGAAA
    ATCAAAGCTG CTTCAGAACT TTATATAGAG ACTGAGAAGA AGAAGCGGGA GAAGGCGTAC
    CTGGTCCGTT CCCAAAGGGC GACAGGTATT GGAAGGTTGA TGGTGAACGT CGTTGAGGGC
    ATTGAGCTGA AACCCTGTCG ATCACATGGA AAGAGTAACC CGTACTGTGA GGTGACCATG
    GGTTCCCAGT GTCACATCAC CAAGACGATC CAGGACACTC TGAATCCCAA GTGGAATTCC
    AACTGCCAGT TCTTCATCAA AGACCTGGAG CAGGAGGTCC TGTGCATCAC CGTGTTTGAG
    AGGGACCAGT TCTCTCCAGA CGATTTTTTG GGTCGGACAG AGATCCGTGT GGCCGACATC
    AAGAAGGACC AGGGTTCCAA GGGTCCAGTT ACAAAGTGTC TCCTGCTACA CGAAGTTCCC
    ACAGGAGAGA TTGTGGTCCG CTTGGACCTG CAGCTGTTTG ATGAGCCATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965222.1
    CDS296..5446
    Translation

    Target ORF information:

    RefSeq Version XM_014109747.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris intersectin 1 (SH3 domain protein) (ITSN1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109747.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGCTCAGT TTCCAACACC TTTTGGAGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAA 
    GAAAGAGCAA AGCATGATCA ACAGTTCCAT AGTTTAAAGC CAATATCTGG ATTTATTACT
    GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG
    ATATGGGCGC TAGCTGACAT GAACAATGAT GGACGAATGG ATCAAGTGGA GTTTTCCATA
    GCTATGAAAC TCATCAAACT CAAGCTGCAA GGATATCAAC TTCCTTCTGC ACTTCCCCCC
    GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CCTTTGGTAT AGGAGGTATT
    GCCAGCATGC CACCACTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTC
    GGAATGTCTC CACCTCTAGT ATCTTCTGTT CCTACAGCAG CAGTGCCTCC CCTGGCTAAC
    GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA
    AAGAGCTCTT CCTTCAGTAG ATCTGGTCCC GGGTCACAAT TAAACACTAA GTTACAGAAG
    GCACAGTCAT TTGATGTGGC TAGTGTCCCT CCCGTGGCAG AATGGGCTGT CCCTCAGTCA
    TCCAGACTTA AATACAGACA GTTATTCAAC AGTCATGACA AAACTATGAG TGGACACTTA
    ACAGGTCCAC AAGCAAGGAC TATTCTTATG CAATCAAGTT TGCCACAGGC TCAGCTGGCT
    TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TCACAGCAGA AGAATTTATC
    TTGGCTATGC ACCTAATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT
    CCAGAGTACA TTCCACCTTC CTTTAGACGA GTTCGATCTG GCAGTGGTAT ATCAGTCATC
    AGCTCAACAG CAGTAGATCA GAGGTTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA
    CAACTAGAAA AGAAATTACC TGTAACCTTT GAAGATAAGA AGCGTGAGAA TTTTGAACGT
    GGCAATCTGG AACTCGAGAA ACGGAGACAA GCGCTCTTGG AGCAGCAGCG CAAAGAGCAA
    GAGCGCCTGG CACAGCTTGA ACGTGCAGAG CAAGAACGGA AAGAGCGGGA GCGCCAGGAG
    CAAGAGCGCA AAAGGCAACT GGAGTTGGAG AAACAACTGG AAAAGCAACG GGAGCTAGAA
    CGTCAGAGAG AAGAGGAGAG GAGGAAAGAA ATTGAGAGAC GAGAGGCTGC AAAGAGAGAA
    CTTGAAAGAC AGCGGCAACT GGAATGGGAA CGAAATCGAA GACAAGAGCT ACTCAATCAG
    AGAAACAAAG AACAAGAAGA CATAGTTGTA CTGAAAGCAA AGAAGAAGAC TCTAGAATTT
    GAATTAGAAG CTCTTAATGA TAAAAAGCAT CAACTAGAAG GAAAACTTCA AGATATCAGG
    TGTCGATTGA CCACCCAACG GCAAGAAATT GAGAGTACAA ATAAATCCAG AGAGTTGAGA
    ATTGCTGAAA TCACCCATCT ACAGCAACAA TTACAGGAAG CCCAGCAAAT GCTTGGAAGA
    CTTATTCCAG AAAAGCAGAT ACTCAATGAC CAATTAAAAC AAGTTCAACA GAACAGTTTG
    CACAGAGATT CACTGCTTAC ACTCAAAAGA GCGTTAGAAG CAAAAGAACT AGCTCGGCAG
    CAGCTACGCG ATCAACTGGA TGAAGTTGAG AAAGAAACCA GATCAAAACT ACAGGAGATT
    GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ATAGTAAGCA ACAACTCCAG
    AAACAAAAGT CCATAGAGGC CGAACGTCTG AAACAGAAAG AACAAGAGCG AAAAACCATA
    GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAGAGGGA CAAGCAGTGG
    ATTGAGCATG TGCAGCAGGA CGATGAGCAT CAGCGGCCAA GGAAGATCCA TGATGAAGAA
    AAACTCCGAA GGGAAGAGAG TGTCAAAAAG AAGGACAGTG AGGAAAAAAG CAAACTGGAC
    ACGCAAGACA AGATGAGTCG GCTTTTCCAC CAGCATCAAG AGCCAGCCAA GCCAGCTGTC
    CAGGCACCCT GGTCCACCAC AGAAAAAGGC CCACTTACAA TTTCTGCACA GGAGAACGTA
    AAGGTGGTGT ATTACCGAGC GCTCTATCCC TTCGAATCCA GAAGTCATGA TGAGATCACG
    ATCCAGCCTG GAGACATAGT AATGGTGGAT GAAAGCCAAA CTGGAGAACC GGGCTGGCTT
    GGAGGAGAAT TAAAAGGAAA GACGGGATGG TTCCCTGCTA ACTACGCAGA GAAAATCCCA
    GAAAACGAGG TGCCCGCTCC AGTCAAACCC GGGCCCGACC TCACCTCTGC CCCTGCGCCC
    AAACTGGCCG TGCGTGAGAC CCCCGCTCCG GTGGCAGTGA GCGCTCCGGA GCCCTCCACG
    ACCCCCAACA GCTGGGCTGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAGCCA
    GAAACCGACA ACTGGGACGC ATGGGCAGCC CAGCCCTCAC TCACTGTTCC CAGTGCTGGC
    CAGCTAAGGC AGAGATCAGC GTTTACTCCA GCCACGGCCA CTGGCTCCTC TCCATCTCCC
    GTTTTAGGCC AGGGTGAGAA GGTGGAAGGG CTACAAGCAC AAGCCCTGTA TCCTTGGAGA
    GCCAAAAAAG ACAACCACTT AAATTTTAAC AAAAATGACG TCATCACCGT CCTGGAACAG
    CAAGACATGT GGTGGTTTGG AGAAGTCCAA GGTCAGAAGG GTTGGTTCCC CAAGTCTTAC
    GTGAAACTCA TTTCAGGGCC CATAAGGAAA TCCACAAGCA TGGATTCTGG TTCTTCAGAA
    AGTCCCGCTA GTCTAAAGAG AGTAGCTTCC CCAGCAGCCA AGCCGGCCAT TTCGGGAGAA
    GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GCGATTTAAC GTTTCAGCAA
    GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAGC AGTGGGCGAC
    AAGTCCGGAG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCAGAGGG CTCTGGAACT
    GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATTG CGCAGGTTAT CGCCTGCTAC
    ACAGCTACTG GTCCCGAGCA GCTCACCCTG GCCCCTGGTC AGCTGATTTT GATCCGAAAA
    AAGAACCCAG GCGGTTGGTG GGAAGGAGAG CTGCAAGCCC GTGGGAAGAA GCGCCAGATA
    GGCTGGTTCC CCGCGAATTA TGTAAAACTT TTGAGCCCTG GCACGAGCAA AATCACTCCA
    ACGGAGCCAC CTAAGCCCAC GGCGTTCCCG GCAGTGTGCC AGGTGATCGG CGTGTACGAC
    TACAGCGCCC AGAACGACGA CGAGCTGGCC TTCAGCAAGG GCCAGATCAT CAACGTCCTC
    AACAAAGACG ACCCGGACTG GTGGAAGGGC GAGGTCAACG GGCAAGTGGG GCTCTTCCCC
    TCCAACTACG TGAAGCTGAC CACGGACATG GACCCAAGCC AGCAATGGTG CTCAGACTTA
    CACCTCTTGG ATATGTTGAC CCCAACGGAG AGAAAGCGAC AAGGATACAT CCATGAGCTC
    ATTGTCACAG AGGAGAACTA TGTGAATGAC CTGCAGCTGG TCACAGAGAT CTTTCAGAAA
    CCCCTGATGG AGTCTGAGCT GCTGACAGAA AAAGAGGGTG CTATGATTTT TGTTAACTGG
    AAGGAGCTGA TTATGTGTAA TATCAAACTA TTGAAAGCGC TGAGGGTCCG CAAGAAGATG
    TCGGGGGAGA AGATGCCCGT GAAGATGATC GGCGACATCC TGACGGCGCA GCTGCCGCAC
    ATGCAGCCTT ACATCCGCTT CTGCAGCTGT CAGCTCAACG GGGCTGCCCT CATCCAGCAG
    AAGACCGACG AGGCCCCGGA CTTCAAGGAG TTTGTCAAAA GGCTGGCAAT GGACCCTCGA
    TGTAAAGGGA TGCCACTCTC TAGTTTTATC CTGAAGCCTA TGCAACGCGT AACGAGATAC
    CCACTGATCA TTAAAAATAT CCTGGAAAAC ACTCCTGAGA ACCACCCCGA CCACAGCCAC
    CTGAAGCACG CCCTGGAGAA GGCCGAAGAG CTGTGTTCAC AGGTGAACGA AGGGGTGCGC
    GAGAAGGAGA ACTCCGACCG CCTCGAGTGG ATCCAGGCGC ACGTGCAGTG CGAAGGCCTG
    TCCGAGCAAC TTGTGTTCAA TTCAGTGACC AATTGCTTGG GGCCACGCAA GTTTCTGCAC
    AGTGGGAAAC TCTACAAGGC CAAGAGCAAC AAGGAGCTGT ATGGCTTCCT CTTCAACGAC
    TTCCTCCTGC TGACCCAAAT CATAAAGCCC TTGGGCTCTT CCGGCACCGA CAAAGTCTTC
    AGTCCCAAAT CTAACCTGCA GTATAAAATG TACAAAACAC CTATTTTCCT GAATGAGGTT
    CTAGTAAAAT TACCCACTGA CCCTTCTGGA GATGAGCCCA TCTTCCACAT CTCCCACATT
    GACCGAGTCT ACACCCTCCG AGCCGAAAGC ATAAATGAAA GGACTGCCTG GGTGCAGAAA
    ATCAAAGCTG CTTCAGAACT TTATATAGAG ACTGAGAAGA AGAAGCGGGA GAAGGCGTAC
    CTGGTCCGTT CCCAAAGGGC GACAGGTATT GGAAGGTTGA TGGTGAACGT CGTTGAGGGC
    ATTGAGCTGA AACCCTGTCG ATCACATGGA AAGAGTAACC CGTACTGTGA GGTGACCATG
    GGTTCCCAGT GTCACATCAC CAAGACGATC CAGGACACTC TGAATCCCAA GTGGAATTCC
    AACTGCCAGT TCTTCATCAA AGACCTGGAG CAGGAGGTCC TGTGCATCAC CGTGTTTGAG
    AGGGACCAGT TCTCTCCAGA CGATTTTTTG GGTCGGACAG AGATCCGTGT GGCCGACATC
    AAGAAGGACC AGGGTTCCAA GGGTCCAGTT ACAAAGTGTC TCCTGCTACA CGAAGTTCCC
    ACAGGAGAGA TTGTGGTCCG CTTGGACCTG CAGCTGTTTG ATGAGCCATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.