USP25 cDNA ORF clone, Canis lupus familiaris(Dog)

The following USP25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the USP25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe283981 XM_022413347.1
Latest version!
Canis lupus familiaris ubiquitin specific peptidase 25 (USP25), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe29296 XM_535562.6
Latest version!
Canis lupus familiaris ubiquitin specific peptidase 25 (USP25), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe29297 XM_003433996.4
Latest version!
Canis lupus familiaris ubiquitin specific peptidase 25 (USP25), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OCe29296 XM_535562.5 Canis lupus familiaris ubiquitin specific peptidase 25 (USP25), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe29297 XM_003433996.3 Canis lupus familiaris ubiquitin specific peptidase 25 (USP25), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe283981
    Clone ID Related Accession (Same CDS sequence) XM_022413347.1
    Accession Version XM_022413347.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2709bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ubiquitin carboxyl-terminal hydrolase 25 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCGTGAGC TGCGGTATCT ATTTGCACTT CTTGTTGGTA CTAAAAGGAA ATATGTTGAC 
    CCATCAAGAG CAGTCGAAAT TCTTAAGGAC GCTTTCAAAT CAAATGACTC CCAGCAGCAA
    GATGTGAGTG AGTTTACACA CAAATTATTA GATTGGTTAG AAGATGCCTT TCAAATGAGA
    GCTGAAGAAG AGACGGATGA AGAGAAGCCA AAGAACCCCA TGGTAGAGTT GTTCTATGGG
    AGGTTCCTTG CTGTGGGAGT ACTTGAAGGT AAAAAATTTG AAAATACTGA AATGTTTGGT
    CAGTACCCAC TTCAGGTCAA TGGATTCAAA GATCTACATG AGTGCCTAGA AGCTGCTATG
    ATTGAAGGAG AAATTGAGTC TTTACATTCA GAGAATTCAG GAAAATCAGG CCAAGAGCAT
    TGGTTCACCA AACTGCCACC TGTGTTAACA TTTGAATTAT CAAGATTTGA ATTCAATCAG
    GCATTGGGAA GACCAGAAAA AATTCACAAC AAATTAGAAT TTCCTCAAGT GTTATATCTG
    GACAGATACA TGCACAGAAA CAGAGAAATA ACAAGAATTA AGAGGGAAGA AATCAAGAGA
    CTAAAAGATT ACCTCACAGT ATTACAACAA AGACTAGAGA GATATTTAAG CTACGGTTCC
    GGTCCCAAAC GATTCCCCTT GGTAGATGTT CTACAGTATG CATTGGAATT TGCTTCAAGT
    AAACCTGTGT GTACATCTCC TGTTGAGGAT ATTGATGCTA GTTCCCCACC TAGTGGTTCC
    ACACCACAGA CATTACCAAG CACAACAGAA CAACAGGGAG TCCCTTCTTC AGAACTGCCA
    AGCACATCAC CTGCATCAAT AGCTGCCATT TCATCAAGAT CAGTAATACA TAAACCATTC
    ACTCAGTCTC GGATACCTCC AGATTTGCCC ATGCATCCAG CACCAAGGCA CATAACAGAA
    GAAGAACTTT CTGTGCTAGA AAGCTGTTTA CATCGCTGGA GGACAGAAAT AGAAAATGAC
    ACCAGAGATT TGCAGGAAAG CATATCCAGA ATCCATCGAA CAATAGAACT AATGTATTCT
    GACAAATCTA TGATCCAAGT TCCTTATCGC TTACATGCTG TTTTAGTTCA TGAAGGTCAA
    GCTAATGCCG GGCACTACTG GGCATACATT TTTGATCATC GTGAAAACAG GTGGATGAAG
    TACAATGATA TTGCTGTGAC AAAATCATCA TGGGAAGAGC TAGTGAGGGA CTCTTTTGGT
    GGTTATAGAA ATGCCAGTGC ATACTGTTTA ATGTACATAA ATGATAAGGC ACAATTCTTA
    ATAAAAGAGG AGTTAAATAA AGAAACTGGG CAGGCTCTTG TTGGAATAGA AACGCTACCA
    CCGGATTTAA GAGATTTTGT TGAGGAAGAC AACCAGAGAT TTGAAAAAGA ACTAGAAGAA
    TGGGATGCAC AGCTTGCTCA GAAAGCTTTG CAGGAAAAAC TTTTAGCATC TCAGAAATTG
    AGAGAGTCAG AGTCTTCTAT AACAACAGCA CAAGCAGGAG AACCAGAATA TCTAGAGCAA
    CCATCAAGAA GTGATTTCTC AAAGCACTTG AAAGAAGAAA CTATTCGAAT AATTACCAAG
    GCATCACATG AACATGAAGA TAAAAGTCCT GAAACAGTTT TGCAGTCGAA ACCTGAAAAT
    ACCACAAGCC AACCACCTTC TAACCAGCAA GTTGTAGAGG TGGCGATTCC TCATGTAGGG
    AAATTCATGA TTGAGTCAAA GGAGGGGGGT TATGATGACG AGATCATGAT GACACCGAAC
    ATGCAAGGTA TTATCATGGC GATAGGTAAA TCCAGGAATG TATATGACAG GTGTGGCCCT
    GAAGCAGGGT TCTTTAAGGC AATTAAGTTG GAGTATTCAA GATTGGTTAA ATTGGCCCAA
    GAAGATACCC CACCAGAAAC AGATTATCGT TTACATCATG TAGTGGTTTA TTTTATCCAG
    AACCAAGCAC CAAAGAAAAT CATTGAGAAA ACATTGCTAG AACAATTTGG AGATAGAAAT
    TTGAGTTTTG ATGAAAGGTG TCACAATATA ATGAAAGTTG CTCAAGCCAA ACTTGAAATG
    ATAAAACCTG AAGAAGTAAA CTTGGAGGAA TATGAGGAGT GGCATCAGGA TTATAGGAAA
    TTCAGGGAAA CAACAATGTA TCTCATAATT GGGCTCGAAA ATTTTCAAAG AGAAAGTTAT
    ATAGATTCCC TGCTGTTCCT TATCTGTGCC TATCAGAACA ACAAAGAACT CTTGTCCAAA
    GGCCCAAACA GAGGACATGA TGAAGAATTG ATATCACATT ATAGAAGAGA ATGTTTACTA
    AAATTAAATG AGCAAGCGGC AGAATTGTTT GAATCTGGAG AGGATCGAGA AGTAAACAAT
    GGTCTGATTA TCATGAACGA GTTTATTGTC CCATTTTTGC CCTTGTTATT GGTGGATGAA
    ATGGAAGAGA AAGATATTCT TGCTGTGGAA GACATGAGAA ATCGATGGTG TTCCTATCTT
    GGACAAGAAA TGGAATCAAA CCTCCAAGAG AAGCTGACAG ATTTTCTGCC AAAACTGCTC
    GATTGTTCTA CTGAGATTAA AGGTTTTCAC GAACCACCGA AGTTACCTTC ATATTCCACA
    CATGAACTGT GTGAGCGGTT TGCCCGAATC ATGTTGTCCC TCAGTCGAAC TCCTGCTGAT
    GGAAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022269055.1
    CDS669..3377
    Translation

    Target ORF information:

    RefSeq Version XM_022413347.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ubiquitin specific peptidase 25 (USP25), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413347.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCGTGAGC TGCGGTATCT ATTTGCACTT CTTGTTGGTA CTAAAAGGAA ATATGTTGAC 
    CCATCAAGAG CAGTCGAAAT TCTTAAGGAC GCTTTCAAAT CAAATGACTC CCAGCAGCAA
    GATGTGAGTG AGTTTACACA CAAATTATTA GATTGGTTAG AAGATGCCTT TCAAATGAGA
    GCTGAAGAAG AGACGGATGA AGAGAAGCCA AAGAACCCCA TGGTAGAGTT GTTCTATGGG
    AGGTTCCTTG CTGTGGGAGT ACTTGAAGGT AAAAAATTTG AAAATACTGA AATGTTTGGT
    CAGTACCCAC TTCAGGTCAA TGGATTCAAA GATCTACATG AGTGCCTAGA AGCTGCTATG
    ATTGAAGGAG AAATTGAGTC TTTACATTCA GAGAATTCAG GAAAATCAGG CCAAGAGCAT
    TGGTTCACCA AACTGCCACC TGTGTTAACA TTTGAATTAT CAAGATTTGA ATTCAATCAG
    GCATTGGGAA GACCAGAAAA AATTCACAAC AAATTAGAAT TTCCTCAAGT GTTATATCTG
    GACAGATACA TGCACAGAAA CAGAGAAATA ACAAGAATTA AGAGGGAAGA AATCAAGAGA
    CTAAAAGATT ACCTCACAGT ATTACAACAA AGACTAGAGA GATATTTAAG CTACGGTTCC
    GGTCCCAAAC GATTCCCCTT GGTAGATGTT CTACAGTATG CATTGGAATT TGCTTCAAGT
    AAACCTGTGT GTACATCTCC TGTTGAGGAT ATTGATGCTA GTTCCCCACC TAGTGGTTCC
    ACACCACAGA CATTACCAAG CACAACAGAA CAACAGGGAG TCCCTTCTTC AGAACTGCCA
    AGCACATCAC CTGCATCAAT AGCTGCCATT TCATCAAGAT CAGTAATACA TAAACCATTC
    ACTCAGTCTC GGATACCTCC AGATTTGCCC ATGCATCCAG CACCAAGGCA CATAACAGAA
    GAAGAACTTT CTGTGCTAGA AAGCTGTTTA CATCGCTGGA GGACAGAAAT AGAAAATGAC
    ACCAGAGATT TGCAGGAAAG CATATCCAGA ATCCATCGAA CAATAGAACT AATGTATTCT
    GACAAATCTA TGATCCAAGT TCCTTATCGC TTACATGCTG TTTTAGTTCA TGAAGGTCAA
    GCTAATGCCG GGCACTACTG GGCATACATT TTTGATCATC GTGAAAACAG GTGGATGAAG
    TACAATGATA TTGCTGTGAC AAAATCATCA TGGGAAGAGC TAGTGAGGGA CTCTTTTGGT
    GGTTATAGAA ATGCCAGTGC ATACTGTTTA ATGTACATAA ATGATAAGGC ACAATTCTTA
    ATAAAAGAGG AGTTAAATAA AGAAACTGGG CAGGCTCTTG TTGGAATAGA AACGCTACCA
    CCGGATTTAA GAGATTTTGT TGAGGAAGAC AACCAGAGAT TTGAAAAAGA ACTAGAAGAA
    TGGGATGCAC AGCTTGCTCA GAAAGCTTTG CAGGAAAAAC TTTTAGCATC TCAGAAATTG
    AGAGAGTCAG AGTCTTCTAT AACAACAGCA CAAGCAGGAG AACCAGAATA TCTAGAGCAA
    CCATCAAGAA GTGATTTCTC AAAGCACTTG AAAGAAGAAA CTATTCGAAT AATTACCAAG
    GCATCACATG AACATGAAGA TAAAAGTCCT GAAACAGTTT TGCAGTCGAA ACCTGAAAAT
    ACCACAAGCC AACCACCTTC TAACCAGCAA GTTGTAGAGG TGGCGATTCC TCATGTAGGG
    AAATTCATGA TTGAGTCAAA GGAGGGGGGT TATGATGACG AGATCATGAT GACACCGAAC
    ATGCAAGGTA TTATCATGGC GATAGGTAAA TCCAGGAATG TATATGACAG GTGTGGCCCT
    GAAGCAGGGT TCTTTAAGGC AATTAAGTTG GAGTATTCAA GATTGGTTAA ATTGGCCCAA
    GAAGATACCC CACCAGAAAC AGATTATCGT TTACATCATG TAGTGGTTTA TTTTATCCAG
    AACCAAGCAC CAAAGAAAAT CATTGAGAAA ACATTGCTAG AACAATTTGG AGATAGAAAT
    TTGAGTTTTG ATGAAAGGTG TCACAATATA ATGAAAGTTG CTCAAGCCAA ACTTGAAATG
    ATAAAACCTG AAGAAGTAAA CTTGGAGGAA TATGAGGAGT GGCATCAGGA TTATAGGAAA
    TTCAGGGAAA CAACAATGTA TCTCATAATT GGGCTCGAAA ATTTTCAAAG AGAAAGTTAT
    ATAGATTCCC TGCTGTTCCT TATCTGTGCC TATCAGAACA ACAAAGAACT CTTGTCCAAA
    GGCCCAAACA GAGGACATGA TGAAGAATTG ATATCACATT ATAGAAGAGA ATGTTTACTA
    AAATTAAATG AGCAAGCGGC AGAATTGTTT GAATCTGGAG AGGATCGAGA AGTAAACAAT
    GGTCTGATTA TCATGAACGA GTTTATTGTC CCATTTTTGC CCTTGTTATT GGTGGATGAA
    ATGGAAGAGA AAGATATTCT TGCTGTGGAA GACATGAGAA ATCGATGGTG TTCCTATCTT
    GGACAAGAAA TGGAATCAAA CCTCCAAGAG AAGCTGACAG ATTTTCTGCC AAAACTGCTC
    GATTGTTCTA CTGAGATTAA AGGTTTTCAC GAACCACCGA AGTTACCTTC ATATTCCACA
    CATGAACTGT GTGAGCGGTT TGCCCGAATC ATGTTGTCCC TCAGTCGAAC TCCTGCTGAT
    GGAAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe29296
    Clone ID Related Accession (Same CDS sequence) XM_535562.6 , XM_535562.5
    Accession Version XM_535562.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3372bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ubiquitin carboxyl-terminal hydrolase 25 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_535562.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
    TTGAATCAGC TGAGAGAAAT CACTGGGATT AATGATGCCC AGATACTACA GCAAGCCTTG
    AAGGATAGTA ATGGAAACTT GGAATTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG
    ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCAC TCCCTGGCAA CGATAGATAC
    ATCAGTGTGG GAAGCCAAGC AGACACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT
    GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCGGAATCAA ACAGGGCATT CAGGGAGACT
    GGAATAACTG ATGAGGAGCA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAAAAT
    AAAGCGTGTT TAAAGAGAAC ACCTACAGAA GTTTGGCGGG ATTCTCGAAA TCCTTATGAT
    AGGAAAAGGC AGGACAAAGC TCCTGTTGGA CTAAAGAATG TTGGCAATAC TTGCTGGTTT
    AGTGCTGTTA TTCAGTCATT ATTCAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC
    AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAATCAAA AGGAGCATCG GAATTTGCCG
    TTTATGCGTG AGCTGCGGTA TCTATTTGCA CTTCTTGTTG GTACTAAAAG GAAATATGTT
    GACCCATCAA GAGCAGTCGA AATTCTTAAG GACGCTTTCA AATCAAATGA CTCCCAGCAG
    CAAGATGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTTCAAATG
    AGAGCTGAAG AAGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT
    GGGAGGTTCC TTGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAATAC TGAAATGTTT
    GGTCAGTACC CACTTCAGGT CAATGGATTC AAAGATCTAC ATGAGTGCCT AGAAGCTGCT
    ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAGAATT CAGGAAAATC AGGCCAAGAG
    CATTGGTTCA CCAAACTGCC ACCTGTGTTA ACATTTGAAT TATCAAGATT TGAATTCAAT
    CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTAG AATTTCCTCA AGTGTTATAT
    CTGGACAGAT ACATGCACAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAAATCAAG
    AGACTAAAAG ATTACCTCAC AGTATTACAA CAAAGACTAG AGAGATATTT AAGCTACGGT
    TCCGGTCCCA AACGATTCCC CTTGGTAGAT GTTCTACAGT ATGCATTGGA ATTTGCTTCA
    AGTAAACCTG TGTGTACATC TCCTGTTGAG GATATTGATG CTAGTTCCCC ACCTAGTGGT
    TCCACACCAC AGACATTACC AAGCACAACA GAACAACAGG GAGTCCCTTC TTCAGAACTG
    CCAAGCACAT CACCTGCATC AATAGCTGCC ATTTCATCAA GATCAGTAAT ACATAAACCA
    TTCACTCAGT CTCGGATACC TCCAGATTTG CCCATGCATC CAGCACCAAG GCACATAACA
    GAAGAAGAAC TTTCTGTGCT AGAAAGCTGT TTACATCGCT GGAGGACAGA AATAGAAAAT
    GACACCAGAG ATTTGCAGGA AAGCATATCC AGAATCCATC GAACAATAGA ACTAATGTAT
    TCTGACAAAT CTATGATCCA AGTTCCTTAT CGCTTACATG CTGTTTTAGT TCATGAAGGT
    CAAGCTAATG CCGGGCACTA CTGGGCATAC ATTTTTGATC ATCGTGAAAA CAGGTGGATG
    AAGTACAATG ATATTGCTGT GACAAAATCA TCATGGGAAG AGCTAGTGAG GGACTCTTTT
    GGTGGTTATA GAAATGCCAG TGCATACTGT TTAATGTACA TAAATGATAA GGCACAATTC
    TTAATAAAAG AGGAGTTAAA TAAAGAAACT GGGCAGGCTC TTGTTGGAAT AGAAACGCTA
    CCACCGGATT TAAGAGATTT TGTTGAGGAA GACAACCAGA GATTTGAAAA AGAACTAGAA
    GAATGGGATG CACAGCTTGC TCAGAAAGCT TTGCAGGAAA AACTTTTAGC ATCTCAGAAA
    TTGAGAGAGT CAGAGTCTTC TATAACAACA GCACAAGCAG GAGAACCAGA ATATCTAGAG
    CAACCATCAA GAAGTGATTT CTCAAAGCAC TTGAAAGAAG AAACTATTCG AATAATTACC
    AAGGCATCAC ATGAACATGA AGATAAAAGT CCTGAAACAG TTTTGCAGTC GAAACCTGAA
    AATACCACAA GCCAACCACC TTCTAACCAG CAAGTTGTAG AGGTGGCGAT TCCTCATGTA
    GGGAAATTCA TGATTGAGTC AAAGGAGGGG GGTTATGATG ACGAGATCAT GATGACACCG
    AACATGCAAG GTATTATCAT GGCGATAGGT AAATCCAGGA ATGTATATGA CAGGTGTGGC
    CCTGAAGCAG GGTTCTTTAA GGCAATTAAG TTGGAGTATT CAAGATTGGT TAAATTGGCC
    CAAGAAGATA CCCCACCAGA AACAGATTAT CGTTTACATC ATGTAGTGGT TTATTTTATC
    CAGAACCAAG CACCAAAGAA AATCATTGAG AAAACATTGC TAGAACAATT TGGAGATAGA
    AATTTGAGTT TTGATGAAAG GTGTCACAAT ATAATGAAAG TTGCTCAAGC CAAACTTGAA
    ATGATAAAAC CTGAAGAAGT AAACTTGGAG GAATATGAGG AGTGGCATCA GGATTATAGG
    AAATTCAGGG AAACAACAAT GTATCTCATA ATTGGGCTCG AAAATTTTCA AAGAGAAAGT
    TATATAGATT CCCTGCTGTT CCTTATCTGT GCCTATCAGA ACAACAAAGA ACTCTTGTCC
    AAAGGCCCAA ACAGAGGACA TGATGAAGAA TTGATATCAC ATTATAGAAG AGAATGTTTA
    CTAAAATTAA ATGAGCAAGC GGCAGAATTG TTTGAATCTG GAGAGGATCG AGAAGTAAAC
    AATGGTCTGA TTATCATGAA CGAGTTTATT GTCCCATTTT TGCCCTTGTT ATTGGTGGAT
    GAAATGGAAG AGAAAGATAT TCTTGCTGTG GAAGACATGA GAAATCGATG GTGTTCCTAT
    CTTGGACAAG AAATGGAATC AAACCTCCAA GAGAAGCTGA CAGATTTTCT GCCAAAACTG
    CTCGATTGTT CTACTGAGAT TAAAGGTTTT CACGAACCAC CGAAGTTACC TTCATATTCC
    ACACATGAAC TGTGTGAGCG GTTTGCCCGA ATCATGTTGT CCCTCAGTCG AACTCCTGCT
    GATGGAAGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_535562.3
    CDS94..3465
    Translation

    Target ORF information:

    RefSeq Version XM_535562.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ubiquitin specific peptidase 25 (USP25), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_535562.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
    TTGAATCAGC TGAGAGAAAT CACTGGGATT AATGATGCCC AGATACTACA GCAAGCCTTG
    AAGGATAGTA ATGGAAACTT GGAATTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG
    ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCAC TCCCTGGCAA CGATAGATAC
    ATCAGTGTGG GAAGCCAAGC AGACACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT
    GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCGGAATCAA ACAGGGCATT CAGGGAGACT
    GGAATAACTG ATGAGGAGCA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAAAAT
    AAAGCGTGTT TAAAGAGAAC ACCTACAGAA GTTTGGCGGG ATTCTCGAAA TCCTTATGAT
    AGGAAAAGGC AGGACAAAGC TCCTGTTGGA CTAAAGAATG TTGGCAATAC TTGCTGGTTT
    AGTGCTGTTA TTCAGTCATT ATTCAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC
    AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAATCAAA AGGAGCATCG GAATTTGCCG
    TTTATGCGTG AGCTGCGGTA TCTATTTGCA CTTCTTGTTG GTACTAAAAG GAAATATGTT
    GACCCATCAA GAGCAGTCGA AATTCTTAAG GACGCTTTCA AATCAAATGA CTCCCAGCAG
    CAAGATGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTTCAAATG
    AGAGCTGAAG AAGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT
    GGGAGGTTCC TTGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAATAC TGAAATGTTT
    GGTCAGTACC CACTTCAGGT CAATGGATTC AAAGATCTAC ATGAGTGCCT AGAAGCTGCT
    ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAGAATT CAGGAAAATC AGGCCAAGAG
    CATTGGTTCA CCAAACTGCC ACCTGTGTTA ACATTTGAAT TATCAAGATT TGAATTCAAT
    CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTAG AATTTCCTCA AGTGTTATAT
    CTGGACAGAT ACATGCACAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAAATCAAG
    AGACTAAAAG ATTACCTCAC AGTATTACAA CAAAGACTAG AGAGATATTT AAGCTACGGT
    TCCGGTCCCA AACGATTCCC CTTGGTAGAT GTTCTACAGT ATGCATTGGA ATTTGCTTCA
    AGTAAACCTG TGTGTACATC TCCTGTTGAG GATATTGATG CTAGTTCCCC ACCTAGTGGT
    TCCACACCAC AGACATTACC AAGCACAACA GAACAACAGG GAGTCCCTTC TTCAGAACTG
    CCAAGCACAT CACCTGCATC AATAGCTGCC ATTTCATCAA GATCAGTAAT ACATAAACCA
    TTCACTCAGT CTCGGATACC TCCAGATTTG CCCATGCATC CAGCACCAAG GCACATAACA
    GAAGAAGAAC TTTCTGTGCT AGAAAGCTGT TTACATCGCT GGAGGACAGA AATAGAAAAT
    GACACCAGAG ATTTGCAGGA AAGCATATCC AGAATCCATC GAACAATAGA ACTAATGTAT
    TCTGACAAAT CTATGATCCA AGTTCCTTAT CGCTTACATG CTGTTTTAGT TCATGAAGGT
    CAAGCTAATG CCGGGCACTA CTGGGCATAC ATTTTTGATC ATCGTGAAAA CAGGTGGATG
    AAGTACAATG ATATTGCTGT GACAAAATCA TCATGGGAAG AGCTAGTGAG GGACTCTTTT
    GGTGGTTATA GAAATGCCAG TGCATACTGT TTAATGTACA TAAATGATAA GGCACAATTC
    TTAATAAAAG AGGAGTTAAA TAAAGAAACT GGGCAGGCTC TTGTTGGAAT AGAAACGCTA
    CCACCGGATT TAAGAGATTT TGTTGAGGAA GACAACCAGA GATTTGAAAA AGAACTAGAA
    GAATGGGATG CACAGCTTGC TCAGAAAGCT TTGCAGGAAA AACTTTTAGC ATCTCAGAAA
    TTGAGAGAGT CAGAGTCTTC TATAACAACA GCACAAGCAG GAGAACCAGA ATATCTAGAG
    CAACCATCAA GAAGTGATTT CTCAAAGCAC TTGAAAGAAG AAACTATTCG AATAATTACC
    AAGGCATCAC ATGAACATGA AGATAAAAGT CCTGAAACAG TTTTGCAGTC GAAACCTGAA
    AATACCACAA GCCAACCACC TTCTAACCAG CAAGTTGTAG AGGTGGCGAT TCCTCATGTA
    GGGAAATTCA TGATTGAGTC AAAGGAGGGG GGTTATGATG ACGAGATCAT GATGACACCG
    AACATGCAAG GTATTATCAT GGCGATAGGT AAATCCAGGA ATGTATATGA CAGGTGTGGC
    CCTGAAGCAG GGTTCTTTAA GGCAATTAAG TTGGAGTATT CAAGATTGGT TAAATTGGCC
    CAAGAAGATA CCCCACCAGA AACAGATTAT CGTTTACATC ATGTAGTGGT TTATTTTATC
    CAGAACCAAG CACCAAAGAA AATCATTGAG AAAACATTGC TAGAACAATT TGGAGATAGA
    AATTTGAGTT TTGATGAAAG GTGTCACAAT ATAATGAAAG TTGCTCAAGC CAAACTTGAA
    ATGATAAAAC CTGAAGAAGT AAACTTGGAG GAATATGAGG AGTGGCATCA GGATTATAGG
    AAATTCAGGG AAACAACAAT GTATCTCATA ATTGGGCTCG AAAATTTTCA AAGAGAAAGT
    TATATAGATT CCCTGCTGTT CCTTATCTGT GCCTATCAGA ACAACAAAGA ACTCTTGTCC
    AAAGGCCCAA ACAGAGGACA TGATGAAGAA TTGATATCAC ATTATAGAAG AGAATGTTTA
    CTAAAATTAA ATGAGCAAGC GGCAGAATTG TTTGAATCTG GAGAGGATCG AGAAGTAAAC
    AATGGTCTGA TTATCATGAA CGAGTTTATT GTCCCATTTT TGCCCTTGTT ATTGGTGGAT
    GAAATGGAAG AGAAAGATAT TCTTGCTGTG GAAGACATGA GAAATCGATG GTGTTCCTAT
    CTTGGACAAG AAATGGAATC AAACCTCCAA GAGAAGCTGA CAGATTTTCT GCCAAAACTG
    CTCGATTGTT CTACTGAGAT TAAAGGTTTT CACGAACCAC CGAAGTTACC TTCATATTCC
    ACACATGAAC TGTGTGAGCG GTTTGCCCGA ATCATGTTGT CCCTCAGTCG AACTCCTGCT
    GATGGAAGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe29296
    Clone ID Related Accession (Same CDS sequence) XM_535562.6 , XM_535562.5
    Accession Version XM_535562.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3372bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ubiquitin carboxyl-terminal hydrolase 25 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_535562.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
    TTGAATCAGC TGAGAGAAAT CACTGGGATT AATGATGCCC AGATACTACA GCAAGCCTTG
    AAGGATAGTA ATGGAAACTT GGAATTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG
    ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCAC TCCCTGGCAA CGATAGATAC
    ATCAGTGTGG GAAGCCAAGC AGACACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT
    GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCGGAATCAA ACAGGGCATT CAGGGAGACT
    GGAATAACTG ATGAGGAGCA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAAAAT
    AAAGCGTGTT TAAAGAGAAC ACCTACAGAA GTTTGGCGGG ATTCTCGAAA TCCTTATGAT
    AGGAAAAGGC AGGACAAAGC TCCTGTTGGA CTAAAGAATG TTGGCAATAC TTGCTGGTTT
    AGTGCTGTTA TTCAGTCATT ATTCAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC
    AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAATCAAA AGGAGCATCG GAATTTGCCG
    TTTATGCGTG AGCTGCGGTA TCTATTTGCA CTTCTTGTTG GTACTAAAAG GAAATATGTT
    GACCCATCAA GAGCAGTCGA AATTCTTAAG GACGCTTTCA AATCAAATGA CTCCCAGCAG
    CAAGATGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTTCAAATG
    AGAGCTGAAG AAGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT
    GGGAGGTTCC TTGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAATAC TGAAATGTTT
    GGTCAGTACC CACTTCAGGT CAATGGATTC AAAGATCTAC ATGAGTGCCT AGAAGCTGCT
    ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAGAATT CAGGAAAATC AGGCCAAGAG
    CATTGGTTCA CCAAACTGCC ACCTGTGTTA ACATTTGAAT TATCAAGATT TGAATTCAAT
    CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTAG AATTTCCTCA AGTGTTATAT
    CTGGACAGAT ACATGCACAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAAATCAAG
    AGACTAAAAG ATTACCTCAC AGTATTACAA CAAAGACTAG AGAGATATTT AAGCTACGGT
    TCCGGTCCCA AACGATTCCC CTTGGTAGAT GTTCTACAGT ATGCATTGGA ATTTGCTTCA
    AGTAAACCTG TGTGTACATC TCCTGTTGAG GATATTGATG CTAGTTCCCC ACCTAGTGGT
    TCCACACCAC AGACATTACC AAGCACAACA GAACAACAGG GAGTCCCTTC TTCAGAACTG
    CCAAGCACAT CACCTGCATC AATAGCTGCC ATTTCATCAA GATCAGTAAT ACATAAACCA
    TTCACTCAGT CTCGGATACC TCCAGATTTG CCCATGCATC CAGCACCAAG GCACATAACA
    GAAGAAGAAC TTTCTGTGCT AGAAAGCTGT TTACATCGCT GGAGGACAGA AATAGAAAAT
    GACACCAGAG ATTTGCAGGA AAGCATATCC AGAATCCATC GAACAATAGA ACTAATGTAT
    TCTGACAAAT CTATGATCCA AGTTCCTTAT CGCTTACATG CTGTTTTAGT TCATGAAGGT
    CAAGCTAATG CCGGGCACTA CTGGGCATAC ATTTTTGATC ATCGTGAAAA CAGGTGGATG
    AAGTACAATG ATATTGCTGT GACAAAATCA TCATGGGAAG AGCTAGTGAG GGACTCTTTT
    GGTGGTTATA GAAATGCCAG TGCATACTGT TTAATGTACA TAAATGATAA GGCACAATTC
    TTAATAAAAG AGGAGTTAAA TAAAGAAACT GGGCAGGCTC TTGTTGGAAT AGAAACGCTA
    CCACCGGATT TAAGAGATTT TGTTGAGGAA GACAACCAGA GATTTGAAAA AGAACTAGAA
    GAATGGGATG CACAGCTTGC TCAGAAAGCT TTGCAGGAAA AACTTTTAGC ATCTCAGAAA
    TTGAGAGAGT CAGAGTCTTC TATAACAACA GCACAAGCAG GAGAACCAGA ATATCTAGAG
    CAACCATCAA GAAGTGATTT CTCAAAGCAC TTGAAAGAAG AAACTATTCG AATAATTACC
    AAGGCATCAC ATGAACATGA AGATAAAAGT CCTGAAACAG TTTTGCAGTC GAAACCTGAA
    AATACCACAA GCCAACCACC TTCTAACCAG CAAGTTGTAG AGGTGGCGAT TCCTCATGTA
    GGGAAATTCA TGATTGAGTC AAAGGAGGGG GGTTATGATG ACGAGATCAT GATGACACCG
    AACATGCAAG GTATTATCAT GGCGATAGGT AAATCCAGGA ATGTATATGA CAGGTGTGGC
    CCTGAAGCAG GGTTCTTTAA GGCAATTAAG TTGGAGTATT CAAGATTGGT TAAATTGGCC
    CAAGAAGATA CCCCACCAGA AACAGATTAT CGTTTACATC ATGTAGTGGT TTATTTTATC
    CAGAACCAAG CACCAAAGAA AATCATTGAG AAAACATTGC TAGAACAATT TGGAGATAGA
    AATTTGAGTT TTGATGAAAG GTGTCACAAT ATAATGAAAG TTGCTCAAGC CAAACTTGAA
    ATGATAAAAC CTGAAGAAGT AAACTTGGAG GAATATGAGG AGTGGCATCA GGATTATAGG
    AAATTCAGGG AAACAACAAT GTATCTCATA ATTGGGCTCG AAAATTTTCA AAGAGAAAGT
    TATATAGATT CCCTGCTGTT CCTTATCTGT GCCTATCAGA ACAACAAAGA ACTCTTGTCC
    AAAGGCCCAA ACAGAGGACA TGATGAAGAA TTGATATCAC ATTATAGAAG AGAATGTTTA
    CTAAAATTAA ATGAGCAAGC GGCAGAATTG TTTGAATCTG GAGAGGATCG AGAAGTAAAC
    AATGGTCTGA TTATCATGAA CGAGTTTATT GTCCCATTTT TGCCCTTGTT ATTGGTGGAT
    GAAATGGAAG AGAAAGATAT TCTTGCTGTG GAAGACATGA GAAATCGATG GTGTTCCTAT
    CTTGGACAAG AAATGGAATC AAACCTCCAA GAGAAGCTGA CAGATTTTCT GCCAAAACTG
    CTCGATTGTT CTACTGAGAT TAAAGGTTTT CACGAACCAC CGAAGTTACC TTCATATTCC
    ACACATGAAC TGTGTGAGCG GTTTGCCCGA ATCATGTTGT CCCTCAGTCG AACTCCTGCT
    GATGGAAGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_535562.3
    CDS86..3457
    Translation

    Target ORF information:

    RefSeq Version XM_535562.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ubiquitin specific peptidase 25 (USP25), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_535562.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
    TTGAATCAGC TGAGAGAAAT CACTGGGATT AATGATGCCC AGATACTACA GCAAGCCTTG
    AAGGATAGTA ATGGAAACTT GGAATTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG
    ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCAC TCCCTGGCAA CGATAGATAC
    ATCAGTGTGG GAAGCCAAGC AGACACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT
    GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCGGAATCAA ACAGGGCATT CAGGGAGACT
    GGAATAACTG ATGAGGAGCA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAAAAT
    AAAGCGTGTT TAAAGAGAAC ACCTACAGAA GTTTGGCGGG ATTCTCGAAA TCCTTATGAT
    AGGAAAAGGC AGGACAAAGC TCCTGTTGGA CTAAAGAATG TTGGCAATAC TTGCTGGTTT
    AGTGCTGTTA TTCAGTCATT ATTCAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC
    AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAATCAAA AGGAGCATCG GAATTTGCCG
    TTTATGCGTG AGCTGCGGTA TCTATTTGCA CTTCTTGTTG GTACTAAAAG GAAATATGTT
    GACCCATCAA GAGCAGTCGA AATTCTTAAG GACGCTTTCA AATCAAATGA CTCCCAGCAG
    CAAGATGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTTCAAATG
    AGAGCTGAAG AAGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT
    GGGAGGTTCC TTGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAATAC TGAAATGTTT
    GGTCAGTACC CACTTCAGGT CAATGGATTC AAAGATCTAC ATGAGTGCCT AGAAGCTGCT
    ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAGAATT CAGGAAAATC AGGCCAAGAG
    CATTGGTTCA CCAAACTGCC ACCTGTGTTA ACATTTGAAT TATCAAGATT TGAATTCAAT
    CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTAG AATTTCCTCA AGTGTTATAT
    CTGGACAGAT ACATGCACAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAAATCAAG
    AGACTAAAAG ATTACCTCAC AGTATTACAA CAAAGACTAG AGAGATATTT AAGCTACGGT
    TCCGGTCCCA AACGATTCCC CTTGGTAGAT GTTCTACAGT ATGCATTGGA ATTTGCTTCA
    AGTAAACCTG TGTGTACATC TCCTGTTGAG GATATTGATG CTAGTTCCCC ACCTAGTGGT
    TCCACACCAC AGACATTACC AAGCACAACA GAACAACAGG GAGTCCCTTC TTCAGAACTG
    CCAAGCACAT CACCTGCATC AATAGCTGCC ATTTCATCAA GATCAGTAAT ACATAAACCA
    TTCACTCAGT CTCGGATACC TCCAGATTTG CCCATGCATC CAGCACCAAG GCACATAACA
    GAAGAAGAAC TTTCTGTGCT AGAAAGCTGT TTACATCGCT GGAGGACAGA AATAGAAAAT
    GACACCAGAG ATTTGCAGGA AAGCATATCC AGAATCCATC GAACAATAGA ACTAATGTAT
    TCTGACAAAT CTATGATCCA AGTTCCTTAT CGCTTACATG CTGTTTTAGT TCATGAAGGT
    CAAGCTAATG CCGGGCACTA CTGGGCATAC ATTTTTGATC ATCGTGAAAA CAGGTGGATG
    AAGTACAATG ATATTGCTGT GACAAAATCA TCATGGGAAG AGCTAGTGAG GGACTCTTTT
    GGTGGTTATA GAAATGCCAG TGCATACTGT TTAATGTACA TAAATGATAA GGCACAATTC
    TTAATAAAAG AGGAGTTAAA TAAAGAAACT GGGCAGGCTC TTGTTGGAAT AGAAACGCTA
    CCACCGGATT TAAGAGATTT TGTTGAGGAA GACAACCAGA GATTTGAAAA AGAACTAGAA
    GAATGGGATG CACAGCTTGC TCAGAAAGCT TTGCAGGAAA AACTTTTAGC ATCTCAGAAA
    TTGAGAGAGT CAGAGTCTTC TATAACAACA GCACAAGCAG GAGAACCAGA ATATCTAGAG
    CAACCATCAA GAAGTGATTT CTCAAAGCAC TTGAAAGAAG AAACTATTCG AATAATTACC
    AAGGCATCAC ATGAACATGA AGATAAAAGT CCTGAAACAG TTTTGCAGTC GAAACCTGAA
    AATACCACAA GCCAACCACC TTCTAACCAG CAAGTTGTAG AGGTGGCGAT TCCTCATGTA
    GGGAAATTCA TGATTGAGTC AAAGGAGGGG GGTTATGATG ACGAGATCAT GATGACACCG
    AACATGCAAG GTATTATCAT GGCGATAGGT AAATCCAGGA ATGTATATGA CAGGTGTGGC
    CCTGAAGCAG GGTTCTTTAA GGCAATTAAG TTGGAGTATT CAAGATTGGT TAAATTGGCC
    CAAGAAGATA CCCCACCAGA AACAGATTAT CGTTTACATC ATGTAGTGGT TTATTTTATC
    CAGAACCAAG CACCAAAGAA AATCATTGAG AAAACATTGC TAGAACAATT TGGAGATAGA
    AATTTGAGTT TTGATGAAAG GTGTCACAAT ATAATGAAAG TTGCTCAAGC CAAACTTGAA
    ATGATAAAAC CTGAAGAAGT AAACTTGGAG GAATATGAGG AGTGGCATCA GGATTATAGG
    AAATTCAGGG AAACAACAAT GTATCTCATA ATTGGGCTCG AAAATTTTCA AAGAGAAAGT
    TATATAGATT CCCTGCTGTT CCTTATCTGT GCCTATCAGA ACAACAAAGA ACTCTTGTCC
    AAAGGCCCAA ACAGAGGACA TGATGAAGAA TTGATATCAC ATTATAGAAG AGAATGTTTA
    CTAAAATTAA ATGAGCAAGC GGCAGAATTG TTTGAATCTG GAGAGGATCG AGAAGTAAAC
    AATGGTCTGA TTATCATGAA CGAGTTTATT GTCCCATTTT TGCCCTTGTT ATTGGTGGAT
    GAAATGGAAG AGAAAGATAT TCTTGCTGTG GAAGACATGA GAAATCGATG GTGTTCCTAT
    CTTGGACAAG AAATGGAATC AAACCTCCAA GAGAAGCTGA CAGATTTTCT GCCAAAACTG
    CTCGATTGTT CTACTGAGAT TAAAGGTTTT CACGAACCAC CGAAGTTACC TTCATATTCC
    ACACATGAAC TGTGTGAGCG GTTTGCCCGA ATCATGTTGT CCCTCAGTCG AACTCCTGCT
    GATGGAAGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe29297
    Clone ID Related Accession (Same CDS sequence) XM_003433996.4 , XM_003433996.3
    Accession Version XM_003433996.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3162bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ubiquitin carboxyl-terminal hydrolase 25 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003433996.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
    TTGAATCAGC TGAGAGAAAT CACTGGGATT AATGATGCCC AGATACTACA GCAAGCCTTG
    AAGGATAGTA ATGGAAACTT GGAATTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG
    ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCAC TCCCTGGCAA CGATAGATAC
    ATCAGTGTGG GAAGCCAAGC AGACACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT
    GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCGGAATCAA ACAGGGCATT CAGGGAGACT
    GGAATAACTG ATGAGGAGCA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAAAAT
    AAAGCGTGTT TAAAGAGAAC ACCTACAGAA GTTTGGCGGG ATTCTCGAAA TCCTTATGAT
    AGGAAAAGGC AGGACAAAGC TCCTGTTGGA CTAAAGAATG TTGGCAATAC TTGCTGGTTT
    AGTGCTGTTA TTCAGTCATT ATTCAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC
    AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAATCAAA AGGAGCATCG GAATTTGCCG
    TTTATGCGTG AGCTGCGGTA TCTATTTGCA CTTCTTGTTG GTACTAAAAG GAAATATGTT
    GACCCATCAA GAGCAGTCGA AATTCTTAAG GACGCTTTCA AATCAAATGA CTCCCAGCAG
    CAAGATGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTTCAAATG
    AGAGCTGAAG AAGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT
    GGGAGGTTCC TTGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAATAC TGAAATGTTT
    GGTCAGTACC CACTTCAGGT CAATGGATTC AAAGATCTAC ATGAGTGCCT AGAAGCTGCT
    ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAGAATT CAGGAAAATC AGGCCAAGAG
    CATTGGTTCA CCAAACTGCC ACCTGTGTTA ACATTTGAAT TATCAAGATT TGAATTCAAT
    CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTAG AATTTCCTCA AGTGTTATAT
    CTGGACAGAT ACATGCACAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAAATCAAG
    AGACTAAAAG ATTACCTCAC AGTATTACAA CAAAGACTAG AGAGATATTT AAGCTACGGT
    TCCGGTCCCA AACGATTCCC CTTGGTAGAT GTTCTACAGT ATGCATTGGA ATTTGCTTCA
    AGTAAACCTG TGTGTACATC TCCTGTTGAG GATATTGATG CTAGTTCCCC ACCTAGTGGT
    TCCACACCAC AGACATTACC AAGCACAACA GAACAACAGG GAGTCCCTTC TTCAGAACTG
    CCAAGCACAT CACCTGCATC AATAGCTGCC ATTTCATCAA GATCAGTAAT ACATAAACCA
    TTCACTCAGT CTCGGATACC TCCAGATTTG CCCATGCATC CAGCACCAAG GCACATAACA
    GAAGAAGAAC TTTCTGTGCT AGAAAGCTGT TTACATCGCT GGAGGACAGA AATAGAAAAT
    GACACCAGAG ATTTGCAGGA AAGCATATCC AGAATCCATC GAACAATAGA ACTAATGTAT
    TCTGACAAAT CTATGATCCA AGTTCCTTAT CGCTTACATG CTGTTTTAGT TCATGAAGGT
    CAAGCTAATG CCGGGCACTA CTGGGCATAC ATTTTTGATC ATCGTGAAAA CAGGTGGATG
    AAGTACAATG ATATTGCTGT GACAAAATCA TCATGGGAAG AGCTAGTGAG GGACTCTTTT
    GGTGGTTATA GAAATGCCAG TGCATACTGT TTAATGTACA TAAATGATAA GGCACAATTC
    TTAATAAAAG AGGAGTTAAA TAAAGAAACT GGGCAGGCTC TTGTTGGAAT AGAAACGCTA
    CCACCGGATT TAAGAGATTT TGTTGAGGAA GACAACCAGA GATTTGAAAA AGAACTAGAA
    GAATGGGATG CACAGCTTGC TCAGAAAGCT TTGCAGGAAA AACTTTTAGC ATCTCAGAAA
    TTGAGAGAGT CAGAGTCTTC TATAACAACA GCACAAGCAG GAGAACCAGA ATATCTAGAG
    CAACCATCAA GAAGTGATTT CTCAAAGCAC TTGAAAGAAG AAACTATTCG AATAATTACC
    AAGGCATCAC ATGAACATGA AGATAAAAGT CCTGAAACAG TTTTGCAGTC GGCAATTAAG
    TTGGAGTATT CAAGATTGGT TAAATTGGCC CAAGAAGATA CCCCACCAGA AACAGATTAT
    CGTTTACATC ATGTAGTGGT TTATTTTATC CAGAACCAAG CACCAAAGAA AATCATTGAG
    AAAACATTGC TAGAACAATT TGGAGATAGA AATTTGAGTT TTGATGAAAG GTGTCACAAT
    ATAATGAAAG TTGCTCAAGC CAAACTTGAA ATGATAAAAC CTGAAGAAGT AAACTTGGAG
    GAATATGAGG AGTGGCATCA GGATTATAGG AAATTCAGGG AAACAACAAT GTATCTCATA
    ATTGGGCTCG AAAATTTTCA AAGAGAAAGT TATATAGATT CCCTGCTGTT CCTTATCTGT
    GCCTATCAGA ACAACAAAGA ACTCTTGTCC AAAGGCCCAA ACAGAGGACA TGATGAAGAA
    TTGATATCAC ATTATAGAAG AGAATGTTTA CTAAAATTAA ATGAGCAAGC GGCAGAATTG
    TTTGAATCTG GAGAGGATCG AGAAGTAAAC AATGGTCTGA TTATCATGAA CGAGTTTATT
    GTCCCATTTT TGCCCTTGTT ATTGGTGGAT GAAATGGAAG AGAAAGATAT TCTTGCTGTG
    GAAGACATGA GAAATCGATG GTGTTCCTAT CTTGGACAAG AAATGGAATC AAACCTCCAA
    GAGAAGCTGA CAGATTTTCT GCCAAAACTG CTCGATTGTT CTACTGAGAT TAAAGGTTTT
    CACGAACCAC CGAAGTTACC TTCATATTCC ACACATGAAC TGTGTGAGCG GTTTGCCCGA
    ATCATGTTGT CCCTCAGTCG AACTCCTGCT GATGGAAGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003434044.1
    CDS93..3254
    Translation

    Target ORF information:

    RefSeq Version XM_003433996.4
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ubiquitin specific peptidase 25 (USP25), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003433996.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
    TTGAATCAGC TGAGAGAAAT CACTGGGATT AATGATGCCC AGATACTACA GCAAGCCTTG
    AAGGATAGTA ATGGAAACTT GGAATTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG
    ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCAC TCCCTGGCAA CGATAGATAC
    ATCAGTGTGG GAAGCCAAGC AGACACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT
    GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCGGAATCAA ACAGGGCATT CAGGGAGACT
    GGAATAACTG ATGAGGAGCA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAAAAT
    AAAGCGTGTT TAAAGAGAAC ACCTACAGAA GTTTGGCGGG ATTCTCGAAA TCCTTATGAT
    AGGAAAAGGC AGGACAAAGC TCCTGTTGGA CTAAAGAATG TTGGCAATAC TTGCTGGTTT
    AGTGCTGTTA TTCAGTCATT ATTCAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC
    AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAATCAAA AGGAGCATCG GAATTTGCCG
    TTTATGCGTG AGCTGCGGTA TCTATTTGCA CTTCTTGTTG GTACTAAAAG GAAATATGTT
    GACCCATCAA GAGCAGTCGA AATTCTTAAG GACGCTTTCA AATCAAATGA CTCCCAGCAG
    CAAGATGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTTCAAATG
    AGAGCTGAAG AAGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT
    GGGAGGTTCC TTGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAATAC TGAAATGTTT
    GGTCAGTACC CACTTCAGGT CAATGGATTC AAAGATCTAC ATGAGTGCCT AGAAGCTGCT
    ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAGAATT CAGGAAAATC AGGCCAAGAG
    CATTGGTTCA CCAAACTGCC ACCTGTGTTA ACATTTGAAT TATCAAGATT TGAATTCAAT
    CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTAG AATTTCCTCA AGTGTTATAT
    CTGGACAGAT ACATGCACAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAAATCAAG
    AGACTAAAAG ATTACCTCAC AGTATTACAA CAAAGACTAG AGAGATATTT AAGCTACGGT
    TCCGGTCCCA AACGATTCCC CTTGGTAGAT GTTCTACAGT ATGCATTGGA ATTTGCTTCA
    AGTAAACCTG TGTGTACATC TCCTGTTGAG GATATTGATG CTAGTTCCCC ACCTAGTGGT
    TCCACACCAC AGACATTACC AAGCACAACA GAACAACAGG GAGTCCCTTC TTCAGAACTG
    CCAAGCACAT CACCTGCATC AATAGCTGCC ATTTCATCAA GATCAGTAAT ACATAAACCA
    TTCACTCAGT CTCGGATACC TCCAGATTTG CCCATGCATC CAGCACCAAG GCACATAACA
    GAAGAAGAAC TTTCTGTGCT AGAAAGCTGT TTACATCGCT GGAGGACAGA AATAGAAAAT
    GACACCAGAG ATTTGCAGGA AAGCATATCC AGAATCCATC GAACAATAGA ACTAATGTAT
    TCTGACAAAT CTATGATCCA AGTTCCTTAT CGCTTACATG CTGTTTTAGT TCATGAAGGT
    CAAGCTAATG CCGGGCACTA CTGGGCATAC ATTTTTGATC ATCGTGAAAA CAGGTGGATG
    AAGTACAATG ATATTGCTGT GACAAAATCA TCATGGGAAG AGCTAGTGAG GGACTCTTTT
    GGTGGTTATA GAAATGCCAG TGCATACTGT TTAATGTACA TAAATGATAA GGCACAATTC
    TTAATAAAAG AGGAGTTAAA TAAAGAAACT GGGCAGGCTC TTGTTGGAAT AGAAACGCTA
    CCACCGGATT TAAGAGATTT TGTTGAGGAA GACAACCAGA GATTTGAAAA AGAACTAGAA
    GAATGGGATG CACAGCTTGC TCAGAAAGCT TTGCAGGAAA AACTTTTAGC ATCTCAGAAA
    TTGAGAGAGT CAGAGTCTTC TATAACAACA GCACAAGCAG GAGAACCAGA ATATCTAGAG
    CAACCATCAA GAAGTGATTT CTCAAAGCAC TTGAAAGAAG AAACTATTCG AATAATTACC
    AAGGCATCAC ATGAACATGA AGATAAAAGT CCTGAAACAG TTTTGCAGTC GGCAATTAAG
    TTGGAGTATT CAAGATTGGT TAAATTGGCC CAAGAAGATA CCCCACCAGA AACAGATTAT
    CGTTTACATC ATGTAGTGGT TTATTTTATC CAGAACCAAG CACCAAAGAA AATCATTGAG
    AAAACATTGC TAGAACAATT TGGAGATAGA AATTTGAGTT TTGATGAAAG GTGTCACAAT
    ATAATGAAAG TTGCTCAAGC CAAACTTGAA ATGATAAAAC CTGAAGAAGT AAACTTGGAG
    GAATATGAGG AGTGGCATCA GGATTATAGG AAATTCAGGG AAACAACAAT GTATCTCATA
    ATTGGGCTCG AAAATTTTCA AAGAGAAAGT TATATAGATT CCCTGCTGTT CCTTATCTGT
    GCCTATCAGA ACAACAAAGA ACTCTTGTCC AAAGGCCCAA ACAGAGGACA TGATGAAGAA
    TTGATATCAC ATTATAGAAG AGAATGTTTA CTAAAATTAA ATGAGCAAGC GGCAGAATTG
    TTTGAATCTG GAGAGGATCG AGAAGTAAAC AATGGTCTGA TTATCATGAA CGAGTTTATT
    GTCCCATTTT TGCCCTTGTT ATTGGTGGAT GAAATGGAAG AGAAAGATAT TCTTGCTGTG
    GAAGACATGA GAAATCGATG GTGTTCCTAT CTTGGACAAG AAATGGAATC AAACCTCCAA
    GAGAAGCTGA CAGATTTTCT GCCAAAACTG CTCGATTGTT CTACTGAGAT TAAAGGTTTT
    CACGAACCAC CGAAGTTACC TTCATATTCC ACACATGAAC TGTGTGAGCG GTTTGCCCGA
    ATCATGTTGT CCCTCAGTCG AACTCCTGCT GATGGAAGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe29297
    Clone ID Related Accession (Same CDS sequence) XM_003433996.4 , XM_003433996.3
    Accession Version XM_003433996.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3162bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ubiquitin carboxyl-terminal hydrolase 25 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003433996.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
    TTGAATCAGC TGAGAGAAAT CACTGGGATT AATGATGCCC AGATACTACA GCAAGCCTTG
    AAGGATAGTA ATGGAAACTT GGAATTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG
    ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCAC TCCCTGGCAA CGATAGATAC
    ATCAGTGTGG GAAGCCAAGC AGACACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT
    GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCGGAATCAA ACAGGGCATT CAGGGAGACT
    GGAATAACTG ATGAGGAGCA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAAAAT
    AAAGCGTGTT TAAAGAGAAC ACCTACAGAA GTTTGGCGGG ATTCTCGAAA TCCTTATGAT
    AGGAAAAGGC AGGACAAAGC TCCTGTTGGA CTAAAGAATG TTGGCAATAC TTGCTGGTTT
    AGTGCTGTTA TTCAGTCATT ATTCAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC
    AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAATCAAA AGGAGCATCG GAATTTGCCG
    TTTATGCGTG AGCTGCGGTA TCTATTTGCA CTTCTTGTTG GTACTAAAAG GAAATATGTT
    GACCCATCAA GAGCAGTCGA AATTCTTAAG GACGCTTTCA AATCAAATGA CTCCCAGCAG
    CAAGATGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTTCAAATG
    AGAGCTGAAG AAGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT
    GGGAGGTTCC TTGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAATAC TGAAATGTTT
    GGTCAGTACC CACTTCAGGT CAATGGATTC AAAGATCTAC ATGAGTGCCT AGAAGCTGCT
    ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAGAATT CAGGAAAATC AGGCCAAGAG
    CATTGGTTCA CCAAACTGCC ACCTGTGTTA ACATTTGAAT TATCAAGATT TGAATTCAAT
    CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTAG AATTTCCTCA AGTGTTATAT
    CTGGACAGAT ACATGCACAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAAATCAAG
    AGACTAAAAG ATTACCTCAC AGTATTACAA CAAAGACTAG AGAGATATTT AAGCTACGGT
    TCCGGTCCCA AACGATTCCC CTTGGTAGAT GTTCTACAGT ATGCATTGGA ATTTGCTTCA
    AGTAAACCTG TGTGTACATC TCCTGTTGAG GATATTGATG CTAGTTCCCC ACCTAGTGGT
    TCCACACCAC AGACATTACC AAGCACAACA GAACAACAGG GAGTCCCTTC TTCAGAACTG
    CCAAGCACAT CACCTGCATC AATAGCTGCC ATTTCATCAA GATCAGTAAT ACATAAACCA
    TTCACTCAGT CTCGGATACC TCCAGATTTG CCCATGCATC CAGCACCAAG GCACATAACA
    GAAGAAGAAC TTTCTGTGCT AGAAAGCTGT TTACATCGCT GGAGGACAGA AATAGAAAAT
    GACACCAGAG ATTTGCAGGA AAGCATATCC AGAATCCATC GAACAATAGA ACTAATGTAT
    TCTGACAAAT CTATGATCCA AGTTCCTTAT CGCTTACATG CTGTTTTAGT TCATGAAGGT
    CAAGCTAATG CCGGGCACTA CTGGGCATAC ATTTTTGATC ATCGTGAAAA CAGGTGGATG
    AAGTACAATG ATATTGCTGT GACAAAATCA TCATGGGAAG AGCTAGTGAG GGACTCTTTT
    GGTGGTTATA GAAATGCCAG TGCATACTGT TTAATGTACA TAAATGATAA GGCACAATTC
    TTAATAAAAG AGGAGTTAAA TAAAGAAACT GGGCAGGCTC TTGTTGGAAT AGAAACGCTA
    CCACCGGATT TAAGAGATTT TGTTGAGGAA GACAACCAGA GATTTGAAAA AGAACTAGAA
    GAATGGGATG CACAGCTTGC TCAGAAAGCT TTGCAGGAAA AACTTTTAGC ATCTCAGAAA
    TTGAGAGAGT CAGAGTCTTC TATAACAACA GCACAAGCAG GAGAACCAGA ATATCTAGAG
    CAACCATCAA GAAGTGATTT CTCAAAGCAC TTGAAAGAAG AAACTATTCG AATAATTACC
    AAGGCATCAC ATGAACATGA AGATAAAAGT CCTGAAACAG TTTTGCAGTC GGCAATTAAG
    TTGGAGTATT CAAGATTGGT TAAATTGGCC CAAGAAGATA CCCCACCAGA AACAGATTAT
    CGTTTACATC ATGTAGTGGT TTATTTTATC CAGAACCAAG CACCAAAGAA AATCATTGAG
    AAAACATTGC TAGAACAATT TGGAGATAGA AATTTGAGTT TTGATGAAAG GTGTCACAAT
    ATAATGAAAG TTGCTCAAGC CAAACTTGAA ATGATAAAAC CTGAAGAAGT AAACTTGGAG
    GAATATGAGG AGTGGCATCA GGATTATAGG AAATTCAGGG AAACAACAAT GTATCTCATA
    ATTGGGCTCG AAAATTTTCA AAGAGAAAGT TATATAGATT CCCTGCTGTT CCTTATCTGT
    GCCTATCAGA ACAACAAAGA ACTCTTGTCC AAAGGCCCAA ACAGAGGACA TGATGAAGAA
    TTGATATCAC ATTATAGAAG AGAATGTTTA CTAAAATTAA ATGAGCAAGC GGCAGAATTG
    TTTGAATCTG GAGAGGATCG AGAAGTAAAC AATGGTCTGA TTATCATGAA CGAGTTTATT
    GTCCCATTTT TGCCCTTGTT ATTGGTGGAT GAAATGGAAG AGAAAGATAT TCTTGCTGTG
    GAAGACATGA GAAATCGATG GTGTTCCTAT CTTGGACAAG AAATGGAATC AAACCTCCAA
    GAGAAGCTGA CAGATTTTCT GCCAAAACTG CTCGATTGTT CTACTGAGAT TAAAGGTTTT
    CACGAACCAC CGAAGTTACC TTCATATTCC ACACATGAAC TGTGTGAGCG GTTTGCCCGA
    ATCATGTTGT CCCTCAGTCG AACTCCTGCT GATGGAAGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003434044.1
    CDS86..3247
    Translation

    Target ORF information:

    RefSeq Version XM_003433996.3
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ubiquitin specific peptidase 25 (USP25), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003433996.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
    TTGAATCAGC TGAGAGAAAT CACTGGGATT AATGATGCCC AGATACTACA GCAAGCCTTG
    AAGGATAGTA ATGGAAACTT GGAATTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG
    ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCAC TCCCTGGCAA CGATAGATAC
    ATCAGTGTGG GAAGCCAAGC AGACACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT
    GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCGGAATCAA ACAGGGCATT CAGGGAGACT
    GGAATAACTG ATGAGGAGCA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAAAAT
    AAAGCGTGTT TAAAGAGAAC ACCTACAGAA GTTTGGCGGG ATTCTCGAAA TCCTTATGAT
    AGGAAAAGGC AGGACAAAGC TCCTGTTGGA CTAAAGAATG TTGGCAATAC TTGCTGGTTT
    AGTGCTGTTA TTCAGTCATT ATTCAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC
    AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAATCAAA AGGAGCATCG GAATTTGCCG
    TTTATGCGTG AGCTGCGGTA TCTATTTGCA CTTCTTGTTG GTACTAAAAG GAAATATGTT
    GACCCATCAA GAGCAGTCGA AATTCTTAAG GACGCTTTCA AATCAAATGA CTCCCAGCAG
    CAAGATGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTTCAAATG
    AGAGCTGAAG AAGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT
    GGGAGGTTCC TTGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAATAC TGAAATGTTT
    GGTCAGTACC CACTTCAGGT CAATGGATTC AAAGATCTAC ATGAGTGCCT AGAAGCTGCT
    ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAGAATT CAGGAAAATC AGGCCAAGAG
    CATTGGTTCA CCAAACTGCC ACCTGTGTTA ACATTTGAAT TATCAAGATT TGAATTCAAT
    CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTAG AATTTCCTCA AGTGTTATAT
    CTGGACAGAT ACATGCACAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAAATCAAG
    AGACTAAAAG ATTACCTCAC AGTATTACAA CAAAGACTAG AGAGATATTT AAGCTACGGT
    TCCGGTCCCA AACGATTCCC CTTGGTAGAT GTTCTACAGT ATGCATTGGA ATTTGCTTCA
    AGTAAACCTG TGTGTACATC TCCTGTTGAG GATATTGATG CTAGTTCCCC ACCTAGTGGT
    TCCACACCAC AGACATTACC AAGCACAACA GAACAACAGG GAGTCCCTTC TTCAGAACTG
    CCAAGCACAT CACCTGCATC AATAGCTGCC ATTTCATCAA GATCAGTAAT ACATAAACCA
    TTCACTCAGT CTCGGATACC TCCAGATTTG CCCATGCATC CAGCACCAAG GCACATAACA
    GAAGAAGAAC TTTCTGTGCT AGAAAGCTGT TTACATCGCT GGAGGACAGA AATAGAAAAT
    GACACCAGAG ATTTGCAGGA AAGCATATCC AGAATCCATC GAACAATAGA ACTAATGTAT
    TCTGACAAAT CTATGATCCA AGTTCCTTAT CGCTTACATG CTGTTTTAGT TCATGAAGGT
    CAAGCTAATG CCGGGCACTA CTGGGCATAC ATTTTTGATC ATCGTGAAAA CAGGTGGATG
    AAGTACAATG ATATTGCTGT GACAAAATCA TCATGGGAAG AGCTAGTGAG GGACTCTTTT
    GGTGGTTATA GAAATGCCAG TGCATACTGT TTAATGTACA TAAATGATAA GGCACAATTC
    TTAATAAAAG AGGAGTTAAA TAAAGAAACT GGGCAGGCTC TTGTTGGAAT AGAAACGCTA
    CCACCGGATT TAAGAGATTT TGTTGAGGAA GACAACCAGA GATTTGAAAA AGAACTAGAA
    GAATGGGATG CACAGCTTGC TCAGAAAGCT TTGCAGGAAA AACTTTTAGC ATCTCAGAAA
    TTGAGAGAGT CAGAGTCTTC TATAACAACA GCACAAGCAG GAGAACCAGA ATATCTAGAG
    CAACCATCAA GAAGTGATTT CTCAAAGCAC TTGAAAGAAG AAACTATTCG AATAATTACC
    AAGGCATCAC ATGAACATGA AGATAAAAGT CCTGAAACAG TTTTGCAGTC GGCAATTAAG
    TTGGAGTATT CAAGATTGGT TAAATTGGCC CAAGAAGATA CCCCACCAGA AACAGATTAT
    CGTTTACATC ATGTAGTGGT TTATTTTATC CAGAACCAAG CACCAAAGAA AATCATTGAG
    AAAACATTGC TAGAACAATT TGGAGATAGA AATTTGAGTT TTGATGAAAG GTGTCACAAT
    ATAATGAAAG TTGCTCAAGC CAAACTTGAA ATGATAAAAC CTGAAGAAGT AAACTTGGAG
    GAATATGAGG AGTGGCATCA GGATTATAGG AAATTCAGGG AAACAACAAT GTATCTCATA
    ATTGGGCTCG AAAATTTTCA AAGAGAAAGT TATATAGATT CCCTGCTGTT CCTTATCTGT
    GCCTATCAGA ACAACAAAGA ACTCTTGTCC AAAGGCCCAA ACAGAGGACA TGATGAAGAA
    TTGATATCAC ATTATAGAAG AGAATGTTTA CTAAAATTAA ATGAGCAAGC GGCAGAATTG
    TTTGAATCTG GAGAGGATCG AGAAGTAAAC AATGGTCTGA TTATCATGAA CGAGTTTATT
    GTCCCATTTT TGCCCTTGTT ATTGGTGGAT GAAATGGAAG AGAAAGATAT TCTTGCTGTG
    GAAGACATGA GAAATCGATG GTGTTCCTAT CTTGGACAAG AAATGGAATC AAACCTCCAA
    GAGAAGCTGA CAGATTTTCT GCCAAAACTG CTCGATTGTT CTACTGAGAT TAAAGGTTTT
    CACGAACCAC CGAAGTTACC TTCATATTCC ACACATGAAC TGTGTGAGCG GTTTGCCCGA
    ATCATGTTGT CCCTCAGTCG AACTCCTGCT GATGGAAGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.