WNK1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	WNK1
Entrez Gene ID	477728
Full Name	WNK lysine deficient protein kinase 1
General protein information	Preferred Names WNK lysine deficient protein kinase 1 Names serine/threonine-protein kinase WNK1
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022411000.1	XP_022266708.1	serine/threonine-protein kinase WNK1 isoform X2
XM_022411001.1	XP_022266709.1	serine/threonine-protein kinase WNK1 isoform X4
XM_014108336.2	XP_013963811.1	serine/threonine-protein kinase WNK1 isoform X10
XM_014108344.2	XP_013963819.1	serine/threonine-protein kinase WNK1 isoform X19
XM_003433482.4	XP_003433530.1	serine/threonine-protein kinase WNK1 isoform X22
XM_014108329.1	XP_013963804.1	serine/threonine-protein kinase WNK1 isoform X6
XM_014108333.1	XP_013963808.1	serine/threonine-protein kinase WNK1 isoform X10
XM_014108334.1	XP_013963809.1	serine/threonine-protein kinase WNK1 isoform X11
XM_014108338.1	XP_013963813.1	serine/threonine-protein kinase WNK1 isoform X14
XM_014108343.1	XP_013963818.1	serine/threonine-protein kinase WNK1 isoform X19
XM_014108332.1	XP_013963807.1	serine/threonine-protein kinase WNK1 isoform X9
XM_014108336.1	XP_013963811.1	serine/threonine-protein kinase WNK1 isoform X13
XM_005637356.3	XP_005637413.1	serine/threonine-protein kinase WNK1 isoform X1
XM_014108334.2	XP_013963809.1	serine/threonine-protein kinase WNK1 isoform X6
XM_022411003.1	XP_022266711.1	serine/threonine-protein kinase WNK1 isoform X7
XM_022411004.1	XP_022266712.1	serine/threonine-protein kinase WNK1 isoform X8
XM_022411009.1	XP_022266717.1	serine/threonine-protein kinase WNK1 isoform X14
XM_014108341.2	XP_013963816.1	serine/threonine-protein kinase WNK1 isoform X15
XM_003433482.3	XP_003433530.1	serine/threonine-protein kinase WNK1 isoform X22
XM_014108339.1	XP_013963814.1	serine/threonine-protein kinase WNK1 isoform X15
XM_014108340.1	XP_013963815.1	serine/threonine-protein kinase WNK1 isoform X16
XM_014108341.1	XP_013963816.1	serine/threonine-protein kinase WNK1 isoform X17
XM_014108328.2	XP_013963803.1	serine/threonine-protein kinase WNK1 isoform X3
XM_022411006.1	XP_022266714.1	serine/threonine-protein kinase WNK1 isoform X9
XM_022411010.1	XP_022266718.1	serine/threonine-protein kinase WNK1 isoform X16
XM_014108343.2	XP_013963818.1	serine/threonine-protein kinase WNK1 isoform X18
XM_022411013.1	XP_022266721.1	serine/threonine-protein kinase WNK1 isoform X21
XM_005637356.2	XP_005637413.1	serine/threonine-protein kinase WNK1 isoform X4
XM_014108327.1	XP_013963802.1	serine/threonine-protein kinase WNK1 isoform X3
XM_014108328.1	XP_013963803.1	serine/threonine-protein kinase WNK1 isoform X5
XM_014108330.1	XP_013963805.1	serine/threonine-protein kinase WNK1 isoform X7
XM_014108331.1	XP_013963806.1	serine/threonine-protein kinase WNK1 isoform X8
XM_014108335.1	XP_013963810.1	serine/threonine-protein kinase WNK1 isoform X12
XM_014108344.1	XP_013963819.1	serine/threonine-protein kinase WNK1 isoform X20
XM_014108338.2	XP_013963813.1	serine/threonine-protein kinase WNK1 isoform X11
XM_022411007.1	XP_022266715.1	serine/threonine-protein kinase WNK1 isoform X12
XM_014108345.2	XP_013963820.1	serine/threonine-protein kinase WNK1 isoform X20
XM_014108325.1	XP_013963800.1	serine/threonine-protein kinase WNK1 isoform X1
XM_014108326.1	XP_013963801.1	serine/threonine-protein kinase WNK1 isoform X2
XM_014108342.1	XP_013963817.1	serine/threonine-protein kinase WNK1 isoform X18
XM_014108345.1	XP_013963820.1	serine/threonine-protein kinase WNK1 isoform X21
XM_022411002.1	XP_022266710.1	serine/threonine-protein kinase WNK1 isoform X5
XM_022411008.1	XP_022266716.1	serine/threonine-protein kinase WNK1 isoform X13
XM_022411011.1	XP_022266719.1	serine/threonine-protein kinase WNK1 isoform X17

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	WNK1	XP_003433530.1
Mus musculus (house mouse)	Wnk1	NP_001186013.1
Bos taurus (cattle)	WNK1	XP_005207339.1
Homo sapiens (human)	WNK1	NP_001171914.1
Rattus norvegicus (Norway rat)	Wnk1	NP_001002823.2

Gene Ontology

The following WNK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WNK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe282750	XM_022411013.1 Latest version!	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe27551	XM_003433482.4 Latest version!	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe27552	XM_005637356.3 Latest version!	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe27553	XM_022411000.1 Latest version!	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe27554	XM_022411003.1 Latest version!	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe27555	XM_022411004.1 Latest version!	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe27556	XM_022411006.1 Latest version!	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe282743	XM_022411001.1 Latest version!	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe282744	XM_022411002.1 Latest version!	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe282745	XM_022411007.1 Latest version!	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe282746	XM_022411008.1 Latest version!	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe282747	XM_022411009.1 Latest version!	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe282748	XM_022411010.1 Latest version!	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe282749	XM_022411011.1 Latest version!	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe43570	XM_014108325.1 Latest version!	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe43571	XM_014108326.1 Latest version!	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe43572	XM_014108327.1 Latest version!	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe43573	XM_014108328.2 Latest version!	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe43574	XM_014108329.1 Latest version!	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe43575	XM_014108330.1 Latest version!	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe43576	XM_014108331.1 Latest version!	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe43577	XM_014108332.1 Latest version!	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe43578	XM_014108333.1 Latest version!	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe43579	XM_014108334.2 Latest version!	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe43580	XM_014108335.1 Latest version!	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe43581	XM_014108336.2 Latest version!	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe43582	XM_014108338.2 Latest version!	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe43583	XM_014108339.1 Latest version!	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe43584	XM_014108340.1 Latest version!	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe43585	XM_014108341.2 Latest version!	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe43586	XM_014108342.1 Latest version!	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe43587	XM_014108343.2 Latest version!	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe43588	XM_014108344.2 Latest version!	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe43589	XM_014108345.2 Latest version!	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
View Old Accession Versions >>
OCe43581	XM_014108336.1	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe43579	XM_014108334.1	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe27551	XM_003433482.3	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe43585	XM_014108341.1	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe43587	XM_014108343.1	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe27552	XM_005637356.2	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe43573	XM_014108328.1	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe43588	XM_014108344.1	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe43582	XM_014108338.1	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCe43589	XM_014108345.1	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

WNK1 ( XM_022411013.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022266721.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X21, mRNA.

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CloneID	OCe282750
Clone ID Related Accession (Same CDS sequence)	XM_022411013.1
Accession Version	XM_022411013.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6117bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase WNK1 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTTA CAAAGAAAGA CTTCTGCTCA GTGTTTGTCA TCATAAATTC TCATTGCTGC 
TGCTGCCCTC AAAAGGATTG TGTAAATGAG GGAGTAAAGC CAGCCAGTTT TGACAAAGTA 
GCAATTCCTG AAGTGAAGGA AATTATTGAA GGATGCATAA GGCAAAACAA AGATGAAAGG 
TATTCTATCA AAGACCTTTT GAACCATGCC TTCTTCCAGG AGGAAACAGG GGTACGGGTA 
GAATTAGCAG AAGAAGATGA TGGAGAAAAG ATAGCCATTA AGTTATGGCT ACGTATTGAA 
GATATTAAGA AATTAAAGGG AAAATACAAA GACAATGAAG CTATTGAGTT TTCCTTTGAC 
TTGGAAAGAG ATGTGCCGGA AGATGTTGCT CAAGAAATGG TAGAGTCTGG GTACGTCTGT 
GAAGGTGATC ACAAGACCAT GGCAAAAGCC ATCAAAGATA GAGTATCATT AATTAAGAGA 
AAACGAGAAC AGCGGCAGTT GGTTCGAGAG GAGCAAGAAA AAAGAAAGCA GGAAGAGGGC 
AGTCTCAAAC AGCAGGTAGA ACAACAATCA AGTACTTCCC AGGCAGGAGT CAAGCAGATC 
CCTTCTGTCA GCACTGGCGT GCCTACTGCT TCTACCACTT CAGCTTCAGT TTCTACGCAA 
GTAGAACCTG AAGAACCTGA GGCAGATCAA CATCAACAGC TACAGTACCA ACAAACTAGT 
ATATCTGTAT TATCTGACGG GACTGTTGAC AGTGGTCAAG GATCTTCTGT CTTCACAGAA 
TCTCGAGTGA GCAGCCAACA GACAGTTTCA TATGGGTCCC AGCATGAACA GGCACATTCT 
ACTGGCACAC TCCCAGGGCA TACAGCTTCT GTTGTCCAGG CACAATCTCA GCCCCATGGG 
GTCTATCCAC CCTCAAGTGT GGGGCAAAGC CAGGGTCAGC CATCCTCAAG TAGCTTAACA 
GGGATTCCAT CTTCCCTCCC CATACAACAT TCTCAGCAGC AGCAGGGAGT ACAGCAGACA 
GCCCCTCCAC AACAGACAGC GCAGTATTCG CTTCCACAGA CATCAGCCTC CAGTGAGGCC 
ACTATTGCAC AACCAGTCAG TCAGTCTCAG GCTCCACAGG TCTTGCCTCA AATATCAGCT 
GGAAAACAGC TTCCAGTTTC CCAGCCAGTA CCAACTATCC AAGGCGAACC TCAGATCCCA 
GTTGCGGCAC AACCCTCAGT TGTTCCAGTC CACTCTGGTG CTCATTTCCT CCCTGTGGGA 
CAGCCACTCT CTACTTCCTT ACTCCCTCAG TACCCGGTCT CTCAAATTCC CATATCAACT 
CCTCATGCGT CTGCGCCCCA GACAGGTTTT TCATCCCTTC CCATTACAAT GGCAGCTGGC 
ATTAATCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTACAG CAGCTGCGAT CCCAGGGGGA 
CCAACTGTGG TTCCTAGTCA GCTTCCAACC CTTCTGCAGC CTGTGACTCA GCTGCCAAGT 
CAGGTTCACC CACAACTCCT GCAACCAGCA GTTCAGTCCA TGGGAATACC AGCTAACCTT 
GGACAAGCTG CTGAGGTTCC ACTTCCCTCT GGAGATGTTC TGTACCAGGG CTTTCCATCT 
CGACTGCCAC CACAGTACCC AGGAGATTCA AATATTGCTC CTTCTTCCAG CGTGGCTTCT 
ATTTGCATCC CTTCTACAGT CCTATCCCCT CCCATGCCCA CAGAAGCACT GGCTACACCG 
GGTTACTTTC CTACAGTGGT GCAGCCTTTT GTGGAATCTA ACCTTTTGGT TCCTGTGGGC 
AGTATAGGAG GACAGGTTCA AGTGTCCCAG CCAGCAGTGA GTTTAGCACA ACAAGCCCCC 
ACTACATCCT CCCAGCAAGC AGCTCTGGAG CAGAATGAAA TGCCAATGTG GGGGCCTTGT 
TGCCACTGGC TGTTCTGTTG GGAAAGCACT CAGGGAGTCT CTCAAGTTGC TCCTCCAGAG 
CCAGTTCCAG TGGCACAGGC ACAGCCTACC ACTTTGGTCT CCTCTATAGA CAGTGCACAT 
TCAGATGTTG CTTCAGGAAT GAGTGATGGC AATGAGAATG TCCCGTCCTC CAGTGGAAGG 
CATGAAGGGA GAGCTACAAA ACGACATTAC CGAAAATCTG TAAGAAGTCG CTCTCGTCAT 
GAAAAGACTT CGCGTCCAAA ATTAAGAATT TTGAATGTTT CAAATAAAGG AGACCGAGTA 
GTAGAATGTC AACTAGAGAC TCATAATCGG AAAATGGTTA CATTCAAATT TGATTTAGAT 
GGTGACAATC CTGAGGAGAT AGCAACAATT ATGGTGAACA ATGACTTTAT TCTAGCAATG 
GAGAGAGAGT CCTTTGTGGA TCAAGTACGA GAAATTATTG AAAAGGCTGA TGAAATGCTC 
AGTGAGGATG TCAGTGTGGA ACCAGAGGGT GACCAGGGAT TGGAGAGTCT GCAGGGAAAG 
GATGACTATG GCTTTACAGG TTCTCAAAAA TTGGAAGGAG AATTCAAACA ACCAGTTCCT 
GCGTCTTCCA TGCCACAGCA AATAGGCATT CCTACCAGTT CTTTAACTCA AGTTGTTCAT 
TCTGCTGGAA GACGGTTTAT AGTGAGTCCT GTGCCAGAAA GTCGATTACG AGAATCAAAA 
GTTTTCACTA GTGAAATACC AGATACAGTT GCTGCCTCTA CTCACAGCGC TGGAATGAAC 
TTGTCTCACT CTGCTTCATC CCTTAGCCTA CAGCAAGCCT TTTCTGAACT TAGACATGTG 
CAGATGACAG AAGGGCCCAG CACAGCACCT CCCAACTTCA GTCATACAGG ACCAACATTT 
CCAGTTGTTC CTCCTTCCAT GAGTAGCATT GCTGGAGTCC CAACCACAGC AGTAGCCACA 
CCTTCAGTAT CAGTCCCTGC AACTAGCAGC CCTCTAAATG ACAGTTCCAC ATCAGTAATT 
CAATCTGAGA TTCCAGTGCC CACTGAAACA GGGGTTGGTG GAGTTCCCAC CTGCACAAGT 
GTAATGCCTT CAAGTGGTCT CCCTGTACCA CCCATATCTG AATCGCCAAT ATTTTCCACT 
ATGGTTTCAA GCATCACAGT ACCTACAGTT GTCTCAATAT CAGCAACATC CCAGCCAGTT 
CAAGCTCCCA CATCTGGGAG CACTGTTTCT AGCATAGGAA CTTTGCCTTC TATACCAGTT 
TCAACAGCTT TAGCCTCTGC AGTGGGCAGT GTTGCTATCC CAGGTGCTAA GCCTCCACCT 
GTACTATCAC AGCAGGCAGC AGGCAGTACT ACTGGGGCAG CCACATTAAC ATCCGTTCCT 
GCCATCACTC CGTTCCCAAG CATAGCTTCA CAGCCATCTC TTCAGCTTAG CAGCAGCACC 
TCTGCTCCAA CTCTAGCTGA AACAGTGGTG GTTAGTGCAC ACTCACTAGA TAAAACATCT 
CATAGCAGTA CAACTGGATT GGCTTTGTCC CTGTCTGCAT CATCTTCCTC CCCTGGAGCA 
GGATTATCTA GTTCTATTTC TCAGCCTGGT GGGGTGCATC CTTTGGTTAC CCCATCAGCT 
ATGGCTTCTA TTCCTGCCCT TCCTCAAACA GCAGGACCTA CTTCTACACC TTTATTGCCC 
CAAGTACTTA GTATCCCACC CTTGGTACAA CCTGTTGCCA GTGTGCCTGC TGTGCAGCAG 
ACACTAATTC ATAGTCAGCC TCAACCAGCT TTACTTCCCA ACCAGCCCCA CACTCACTGT 
CCTGAAATAG ATGCTGACTT ACAGCCCAAA GCTCCTGGAA TTGATGACAT AAAGACTCTA 
GAGGAAAAGC TGCGGTCTCT CTTTAGTGAA CCGAGCTCAT CTGGAGCCCA ACATACATCG 
GTCTCACTAG AGACTTCACT GGTAGTAGAG ACCACTGTCA CGCCAGGTAT CCCAACCACT 
GCTATTGCAC CAAGCAAGCT CATGACATCT ACTACAAGTA CATGCTTACC ACCAACCAGT 
TTGCAATTAG GAACATCTGG TTTGTCAGTT ATACCAGTGG TCACGCCTGG GCAGGTTTCT 
ACCCCAGTCA GCTATGTGTC AGCCCCAGGC AGCACTACAT CAGGAGTGAA AGCTGGAACT 
GCTCCATCAA AGCCACCTTT GACTAAAGCT CCAGTGCTGC CAGTGGGTAC TGAACTTCCA 
GCTGGTACTC CACCCAGCGA GCAGCTGCCA CCTTTTCCAG GACCTTCACT AACTCAGTCC 
CAGCAACCTC TGGAAGATCT TGATGCTCAG TTGAGAAGAA CACTTAGTCC AGAGACTATT 
GCGGTGACAT CTACAATTGG TCCTGTGTCC ATGGTGGCTC CAACAGCAGT CACAGAAGCA 
GGAGCACAGC CTCAGAAGGA TGTTTCTCAG ATCACAGAAG GTCCTGTCCT AGCGACTAGT 
TCGGGAACTG GTGTTTTTAA GATGGGAAGA TTTCAGGTTT CAGTTGCAAT GGATGATGTT 
CAAAAGGAGG GTAAAAATAA GTTAGAAGAT GGAAAGTCTG TTCATTTTGA ATCCAGCACT 
TCAGAGTCCT CAGTGCTATC AAGTAGTAGT CCAGAAAGTA CCCTGGTGAA GACAGAGCCT 
AATGGGATAA CCATCCCTGG CATCTCATCA GATATGCCAG ATAGTGCCCA CAAAACTCCT 
GCCTCAGAAG CAAAGTCAGA AACTGGGCAG CCTACCAAGG TTGGACGTTT TCAGGTGACA 
ACTACAGCAA ACAAAGTAGG TCGTTTCTCT GTGTCAAGAA CTGAAGACAA GCTCATTGAG 
GCAAAGAAAG AGGGACCAAT AGCGTCTCCT CCTTTTATGG ATTTGGAACA AGGTGTTCTT 
CCAGCTGTGA TACCAAAGAA AGAAAAGCCT GAACTGTCAG AGCCTTCACA TCTGAACGGA 
CCATCTTCTG ACCTGGAGGC TGCCTTCCTG AGCAGGGATG TGGATGATGG TTCAGGTAGT 
CCACACTCCC CCCATCAGCT GAGCTCAAAG AGCCTGCCTA TCCAGAATCT AAGTCAAAGC 
CTTAGTAATT CATTCAACTC CTCTTACATG AGTAGTGACA ATGAGTCAGA TATTGAAGAT 
GAAGATTTAA AATTAGAGCT GCGAAGACTA CGAGAAAAAC ATCTTAAAGA GATTCAAGAC 
CTGCAGAGTC GCCAGAAGCA TGAAATTGAA TCTTTGTATA CCAAACTGGG AAAGGTTCCC 
CCTGCTGTCA TTATCCCCCC AGCTGCCCCC CTTTCAGGGA GAAGAAGGCG ACCCACTAAA 
AGCAAAGGCA GCAAGTCTAG TCGCAGCAGT TCCTTGGGGA ATAAAAGTCC CCAGCTTTCA 
GGTAACCTGT CTGGTCAGAG TGCTGCTTCA GTTTTGCACG CTCAGCAGAC CCTCCACCCT 
CCTGGCAACA TCCCTGAGAC TGGGCCAAAT CAGCTGTTAC AGCCCCTTAA GCCATCTCCC 
TCCAGTGACA ACCTCTACTC AGCCTTTACC AGTGATGGTG CCATTTCAGT CCCAAGCCTT 
TCTGCTCCAG GTCAAGGCTG TGCGAAGTTT AACTGTGCAT CTGAGCAGGT GACATTCAAA 
CCTGGTGGCA GGAGGACCCG ATTTCTGAGT ACGCCCTGCT TGGCTCTTTG GAAGATGGTG 
AAAAAAGTCT GTCCTTGCAA CCAGCTCTGT AGGACCAGCA GCACAAACAC TGTCGGGGGA 
ACAGTGAACA GCCAAGCTGC CCCAGCTCAG CCTCCTGCCA TGACATCCAG CAGGAAGGGC 
ACATTCACAG ATGACCTACA CAAGTTGGTA GACAATTGGG CCCGAGATGC CATGAATCTC 
TCAGGCAGAA GAGGAAGCAA AGGACACATG AATTATGAGG GCCCTGGAAT GGCAAGAAAG 
TTCTCCGCAC CTGGTCAGCT GTGCATCTCC ATGACCTCAA ATCTGGGTGG TTCTGCCCCC 
ATCTCTGCAG CATCAGCTAC CTCTCTAGGT CACTTCACCA AGTCTATGTG CCCCCCACAG 
CAGTACGGTT TTCCAGCTCC CCCCTTTGGC ACTCAGTGGA GTGGGACGGG TGGCCCGGCA 
CCACAGCCAC TTGGCCAGTT CCAGCCTGTG GGAACTGCCT CCTTACAGAA TTTCAACATC 
AGCAATTTGC AGAAATCCAT CAGCAACCCC CCAGGTTCCA ACCTGCGGAC CACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022266721.1
CDS	296..6412
Translation	MDFTKKDFCSVFVIINSHCCCCPQKDCVNEGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVGQSQGQPSSSSLTGIPSSLPIQHSQQQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQLPVSQPVPTIQGEPQIPVAAQPSVVPVHSGAHFLPVGQPLSTSLLPQYPVSQIPISTPHASAPQTGFSSLPITMAAGINQPLLTLASSATAAAIPGGPTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPSRLPPQYPGDSNIAPSSSVASICIPSTVLSPPMPTEALATPGYFPTVVQPFVESNLLVPVGSIGGQVQVSQPAVSLAQQAPTTSSQQAALEQNEMPMWGPCCHWLFCWESTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGCAKFNCASEQVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_022411013.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022411013.1

ORF Insert Sequence:

ATGGATTTTA CAAAGAAAGA CTTCTGCTCA GTGTTTGTCA TCATAAATTC TCATTGCTGC 
TGCTGCCCTC AAAAGGATTG TGTAAATGAG GGAGTAAAGC CAGCCAGTTT TGACAAAGTA 
GCAATTCCTG AAGTGAAGGA AATTATTGAA GGATGCATAA GGCAAAACAA AGATGAAAGG 
TATTCTATCA AAGACCTTTT GAACCATGCC TTCTTCCAGG AGGAAACAGG GGTACGGGTA 
GAATTAGCAG AAGAAGATGA TGGAGAAAAG ATAGCCATTA AGTTATGGCT ACGTATTGAA 
GATATTAAGA AATTAAAGGG AAAATACAAA GACAATGAAG CTATTGAGTT TTCCTTTGAC 
TTGGAAAGAG ATGTGCCGGA AGATGTTGCT CAAGAAATGG TAGAGTCTGG GTACGTCTGT 
GAAGGTGATC ACAAGACCAT GGCAAAAGCC ATCAAAGATA GAGTATCATT AATTAAGAGA 
AAACGAGAAC AGCGGCAGTT GGTTCGAGAG GAGCAAGAAA AAAGAAAGCA GGAAGAGGGC 
AGTCTCAAAC AGCAGGTAGA ACAACAATCA AGTACTTCCC AGGCAGGAGT CAAGCAGATC 
CCTTCTGTCA GCACTGGCGT GCCTACTGCT TCTACCACTT CAGCTTCAGT TTCTACGCAA 
GTAGAACCTG AAGAACCTGA GGCAGATCAA CATCAACAGC TACAGTACCA ACAAACTAGT 
ATATCTGTAT TATCTGACGG GACTGTTGAC AGTGGTCAAG GATCTTCTGT CTTCACAGAA 
TCTCGAGTGA GCAGCCAACA GACAGTTTCA TATGGGTCCC AGCATGAACA GGCACATTCT 
ACTGGCACAC TCCCAGGGCA TACAGCTTCT GTTGTCCAGG CACAATCTCA GCCCCATGGG 
GTCTATCCAC CCTCAAGTGT GGGGCAAAGC CAGGGTCAGC CATCCTCAAG TAGCTTAACA 
GGGATTCCAT CTTCCCTCCC CATACAACAT TCTCAGCAGC AGCAGGGAGT ACAGCAGACA 
GCCCCTCCAC AACAGACAGC GCAGTATTCG CTTCCACAGA CATCAGCCTC CAGTGAGGCC 
ACTATTGCAC AACCAGTCAG TCAGTCTCAG GCTCCACAGG TCTTGCCTCA AATATCAGCT 
GGAAAACAGC TTCCAGTTTC CCAGCCAGTA CCAACTATCC AAGGCGAACC TCAGATCCCA 
GTTGCGGCAC AACCCTCAGT TGTTCCAGTC CACTCTGGTG CTCATTTCCT CCCTGTGGGA 
CAGCCACTCT CTACTTCCTT ACTCCCTCAG TACCCGGTCT CTCAAATTCC CATATCAACT 
CCTCATGCGT CTGCGCCCCA GACAGGTTTT TCATCCCTTC CCATTACAAT GGCAGCTGGC 
ATTAATCAGC CTCTGCTCAC CTTGGCTTCA TCTGCTACAG CAGCTGCGAT CCCAGGGGGA 
CCAACTGTGG TTCCTAGTCA GCTTCCAACC CTTCTGCAGC CTGTGACTCA GCTGCCAAGT 
CAGGTTCACC CACAACTCCT GCAACCAGCA GTTCAGTCCA TGGGAATACC AGCTAACCTT 
GGACAAGCTG CTGAGGTTCC ACTTCCCTCT GGAGATGTTC TGTACCAGGG CTTTCCATCT 
CGACTGCCAC CACAGTACCC AGGAGATTCA AATATTGCTC CTTCTTCCAG CGTGGCTTCT 
ATTTGCATCC CTTCTACAGT CCTATCCCCT CCCATGCCCA CAGAAGCACT GGCTACACCG 
GGTTACTTTC CTACAGTGGT GCAGCCTTTT GTGGAATCTA ACCTTTTGGT TCCTGTGGGC 
AGTATAGGAG GACAGGTTCA AGTGTCCCAG CCAGCAGTGA GTTTAGCACA ACAAGCCCCC 
ACTACATCCT CCCAGCAAGC AGCTCTGGAG CAGAATGAAA TGCCAATGTG GGGGCCTTGT 
TGCCACTGGC TGTTCTGTTG GGAAAGCACT CAGGGAGTCT CTCAAGTTGC TCCTCCAGAG 
CCAGTTCCAG TGGCACAGGC ACAGCCTACC ACTTTGGTCT CCTCTATAGA CAGTGCACAT 
TCAGATGTTG CTTCAGGAAT GAGTGATGGC AATGAGAATG TCCCGTCCTC CAGTGGAAGG 
CATGAAGGGA GAGCTACAAA ACGACATTAC CGAAAATCTG TAAGAAGTCG CTCTCGTCAT 
GAAAAGACTT CGCGTCCAAA ATTAAGAATT TTGAATGTTT CAAATAAAGG AGACCGAGTA 
GTAGAATGTC AACTAGAGAC TCATAATCGG AAAATGGTTA CATTCAAATT TGATTTAGAT 
GGTGACAATC CTGAGGAGAT AGCAACAATT ATGGTGAACA ATGACTTTAT TCTAGCAATG 
GAGAGAGAGT CCTTTGTGGA TCAAGTACGA GAAATTATTG AAAAGGCTGA TGAAATGCTC 
AGTGAGGATG TCAGTGTGGA ACCAGAGGGT GACCAGGGAT TGGAGAGTCT GCAGGGAAAG 
GATGACTATG GCTTTACAGG TTCTCAAAAA TTGGAAGGAG AATTCAAACA ACCAGTTCCT 
GCGTCTTCCA TGCCACAGCA AATAGGCATT CCTACCAGTT CTTTAACTCA AGTTGTTCAT 
TCTGCTGGAA GACGGTTTAT AGTGAGTCCT GTGCCAGAAA GTCGATTACG AGAATCAAAA 
GTTTTCACTA GTGAAATACC AGATACAGTT GCTGCCTCTA CTCACAGCGC TGGAATGAAC 
TTGTCTCACT CTGCTTCATC CCTTAGCCTA CAGCAAGCCT TTTCTGAACT TAGACATGTG 
CAGATGACAG AAGGGCCCAG CACAGCACCT CCCAACTTCA GTCATACAGG ACCAACATTT 
CCAGTTGTTC CTCCTTCCAT GAGTAGCATT GCTGGAGTCC CAACCACAGC AGTAGCCACA 
CCTTCAGTAT CAGTCCCTGC AACTAGCAGC CCTCTAAATG ACAGTTCCAC ATCAGTAATT 
CAATCTGAGA TTCCAGTGCC CACTGAAACA GGGGTTGGTG GAGTTCCCAC CTGCACAAGT 
GTAATGCCTT CAAGTGGTCT CCCTGTACCA CCCATATCTG AATCGCCAAT ATTTTCCACT 
ATGGTTTCAA GCATCACAGT ACCTACAGTT GTCTCAATAT CAGCAACATC CCAGCCAGTT 
CAAGCTCCCA CATCTGGGAG CACTGTTTCT AGCATAGGAA CTTTGCCTTC TATACCAGTT 
TCAACAGCTT TAGCCTCTGC AGTGGGCAGT GTTGCTATCC CAGGTGCTAA GCCTCCACCT 
GTACTATCAC AGCAGGCAGC AGGCAGTACT ACTGGGGCAG CCACATTAAC ATCCGTTCCT 
GCCATCACTC CGTTCCCAAG CATAGCTTCA CAGCCATCTC TTCAGCTTAG CAGCAGCACC 
TCTGCTCCAA CTCTAGCTGA AACAGTGGTG GTTAGTGCAC ACTCACTAGA TAAAACATCT 
CATAGCAGTA CAACTGGATT GGCTTTGTCC CTGTCTGCAT CATCTTCCTC CCCTGGAGCA 
GGATTATCTA GTTCTATTTC TCAGCCTGGT GGGGTGCATC CTTTGGTTAC CCCATCAGCT 
ATGGCTTCTA TTCCTGCCCT TCCTCAAACA GCAGGACCTA CTTCTACACC TTTATTGCCC 
CAAGTACTTA GTATCCCACC CTTGGTACAA CCTGTTGCCA GTGTGCCTGC TGTGCAGCAG 
ACACTAATTC ATAGTCAGCC TCAACCAGCT TTACTTCCCA ACCAGCCCCA CACTCACTGT 
CCTGAAATAG ATGCTGACTT ACAGCCCAAA GCTCCTGGAA TTGATGACAT AAAGACTCTA 
GAGGAAAAGC TGCGGTCTCT CTTTAGTGAA CCGAGCTCAT CTGGAGCCCA ACATACATCG 
GTCTCACTAG AGACTTCACT GGTAGTAGAG ACCACTGTCA CGCCAGGTAT CCCAACCACT 
GCTATTGCAC CAAGCAAGCT CATGACATCT ACTACAAGTA CATGCTTACC ACCAACCAGT 
TTGCAATTAG GAACATCTGG TTTGTCAGTT ATACCAGTGG TCACGCCTGG GCAGGTTTCT 
ACCCCAGTCA GCTATGTGTC AGCCCCAGGC AGCACTACAT CAGGAGTGAA AGCTGGAACT 
GCTCCATCAA AGCCACCTTT GACTAAAGCT CCAGTGCTGC CAGTGGGTAC TGAACTTCCA 
GCTGGTACTC CACCCAGCGA GCAGCTGCCA CCTTTTCCAG GACCTTCACT AACTCAGTCC 
CAGCAACCTC TGGAAGATCT TGATGCTCAG TTGAGAAGAA CACTTAGTCC AGAGACTATT 
GCGGTGACAT CTACAATTGG TCCTGTGTCC ATGGTGGCTC CAACAGCAGT CACAGAAGCA 
GGAGCACAGC CTCAGAAGGA TGTTTCTCAG ATCACAGAAG GTCCTGTCCT AGCGACTAGT 
TCGGGAACTG GTGTTTTTAA GATGGGAAGA TTTCAGGTTT CAGTTGCAAT GGATGATGTT 
CAAAAGGAGG GTAAAAATAA GTTAGAAGAT GGAAAGTCTG TTCATTTTGA ATCCAGCACT 
TCAGAGTCCT CAGTGCTATC AAGTAGTAGT CCAGAAAGTA CCCTGGTGAA GACAGAGCCT 
AATGGGATAA CCATCCCTGG CATCTCATCA GATATGCCAG ATAGTGCCCA CAAAACTCCT 
GCCTCAGAAG CAAAGTCAGA AACTGGGCAG CCTACCAAGG TTGGACGTTT TCAGGTGACA 
ACTACAGCAA ACAAAGTAGG TCGTTTCTCT GTGTCAAGAA CTGAAGACAA GCTCATTGAG 
GCAAAGAAAG AGGGACCAAT AGCGTCTCCT CCTTTTATGG ATTTGGAACA AGGTGTTCTT 
CCAGCTGTGA TACCAAAGAA AGAAAAGCCT GAACTGTCAG AGCCTTCACA TCTGAACGGA 
CCATCTTCTG ACCTGGAGGC TGCCTTCCTG AGCAGGGATG TGGATGATGG TTCAGGTAGT 
CCACACTCCC CCCATCAGCT GAGCTCAAAG AGCCTGCCTA TCCAGAATCT AAGTCAAAGC 
CTTAGTAATT CATTCAACTC CTCTTACATG AGTAGTGACA ATGAGTCAGA TATTGAAGAT 
GAAGATTTAA AATTAGAGCT GCGAAGACTA CGAGAAAAAC ATCTTAAAGA GATTCAAGAC 
CTGCAGAGTC GCCAGAAGCA TGAAATTGAA TCTTTGTATA CCAAACTGGG AAAGGTTCCC 
CCTGCTGTCA TTATCCCCCC AGCTGCCCCC CTTTCAGGGA GAAGAAGGCG ACCCACTAAA 
AGCAAAGGCA GCAAGTCTAG TCGCAGCAGT TCCTTGGGGA ATAAAAGTCC CCAGCTTTCA 
GGTAACCTGT CTGGTCAGAG TGCTGCTTCA GTTTTGCACG CTCAGCAGAC CCTCCACCCT 
CCTGGCAACA TCCCTGAGAC TGGGCCAAAT CAGCTGTTAC AGCCCCTTAA GCCATCTCCC 
TCCAGTGACA ACCTCTACTC AGCCTTTACC AGTGATGGTG CCATTTCAGT CCCAAGCCTT 
TCTGCTCCAG GTCAAGGCTG TGCGAAGTTT AACTGTGCAT CTGAGCAGGT GACATTCAAA 
CCTGGTGGCA GGAGGACCCG ATTTCTGAGT ACGCCCTGCT TGGCTCTTTG GAAGATGGTG 
AAAAAAGTCT GTCCTTGCAA CCAGCTCTGT AGGACCAGCA GCACAAACAC TGTCGGGGGA 
ACAGTGAACA GCCAAGCTGC CCCAGCTCAG CCTCCTGCCA TGACATCCAG CAGGAAGGGC 
ACATTCACAG ATGACCTACA CAAGTTGGTA GACAATTGGG CCCGAGATGC CATGAATCTC 
TCAGGCAGAA GAGGAAGCAA AGGACACATG AATTATGAGG GCCCTGGAAT GGCAAGAAAG 
TTCTCCGCAC CTGGTCAGCT GTGCATCTCC ATGACCTCAA ATCTGGGTGG TTCTGCCCCC 
ATCTCTGCAG CATCAGCTAC CTCTCTAGGT CACTTCACCA AGTCTATGTG CCCCCCACAG 
CAGTACGGTT TTCCAGCTCC CCCCTTTGGC ACTCAGTGGA GTGGGACGGG TGGCCCGGCA 
CCACAGCCAC TTGGCCAGTT CCAGCCTGTG GGAACTGCCT CCTTACAGAA TTTCAACATC 
AGCAATTTGC AGAAATCCAT CAGCAACCCC CCAGGTTCCA ACCTGCGGAC CACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_003433482.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003433530.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X22, mRNA.

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CloneID	OCe27551
Clone ID Related Accession (Same CDS sequence)	XM_003433482.4 , XM_003433482.3
Accession Version	XM_003433482.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase WNK1 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003433482.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGCCT 
CAGTCCATGG CGCATCCGTG TGGGGGGACC CCAACATACC CAGAATCACA GATATTTTTC 
CCAACTATTC ATGAACGTCC AGTTTCTTTT TCACCACCTC CCACCTGTCC ACCGAAGGTG 
GCCATTTCCC AGCGACGTAA GAGCACCTCC TTCTTGGAAG CCCAAACTCG CCATTTCCAA 
CCCCTGCTGA GGACTGTTGG CCAAAATCTT CTTCCACCTG GTGGCAGCCC AACTAACTGG 
ACACCAGAGG CTGTAGTTAT GTTGGGCACT ACAGCCAGTA GAATAACTGG AGAGCCATGT 
GAGATACAGG TCCATCCTAT GTTTGAACCA ACTCAAGTTC ACAGTGACTA TAGACCTGGA 
CTGGTACTTC CAGAAGAAGC TCACTATTTT ATTCCTCAGG AAGCAATGTA TGTAGCTTAC 
CAGGCCCAGG TGACAGAACA GTATGAGGGT ATTCCATACA ACTCACCAGT ACCGTCAAGT 
CCTATGAAAC AGATACCTGA ACAGAAGCCA GTACAAGGGG GCCCTACTTC AAGTTCTGTC 
TTTGAATTTC CATCTGGACA GGCTTTCCTG GTAGGACACC TTCAAAATCT AAGGTTAGGT 
TCTGGATTGA GTCCAGGATC TCCCCTCTCT AGTATTTCTG CACCTATCAG TACAGATGCT 
ACATGTTTGA AATTTCACCC TGTCTTTGTT CCTCATTCTG CGCCTGCTGT GTTAACTCAT 
AACAATGAGA GCAGAAGCAA TTGTGTATTT GAGTTTCATG TTCATACTCC AAGCTCCTCT 
TCAGGAGAAG GAGGAGGAAT TTTACCTCAG CGTGTTTACC GAAATCGGCA GGTTGCAGTG 
GACTTGAATC AGGAAGAACC ACCTCCTCAA TCAGTTGGAT TACACGGCCG CCTACAGCCT 
GTGACTGAAG AACAGCGTAA TTACCATGCC CCAGATTTGA CCATTTCTGT GGTAGAGCCT 
ATCGGACAGA CCTGGCCAAT AGGAAGCCCA GAATACTCCA GTGATTCCTC ACAAATTACT 
TCTTCAGACC CCAGTGATTT TCAATCACCT CCCCCTACAG GGGGAGCAGC TGTACCATTT 
GGCTCTGACG TCTCATTACC CTTTATCCAT CTGCCTCAGA CAGTGTTACA AGAATCCCCA 
CTTTTCTTCT GTTTCCCCCA AGGAACCACA TCTCAGCAGG TTTTATCTGC CTCATTTTCT 
TCAGGAGGAT CTGCACTCCA TCCACAGGCA CAGGGGCAAA GCCAGGGTCA GCCATCCTCA 
AGTAGCTTAA CAGGGATTCC ATCTTCCCTC CCCATACAAC ATTCTCAGCA GCAGCAGGGA 
GTACAGCAGA CAGCCCCTCC ACAACAGACA GCGCAGTATT CGCTTCCACA GACATCAGCC 
TCCAGTGAGG CCACTATTGC ACAACCAGTC AGTCAGTCTC AGGCTCCACA GGTCTTGCCT 
CAAATATCAG CTGGAAAACA GGGCTTTCCA TCTCGACTGC CACCACAGTA CCCAGGAGAT 
TCAAATATTG CTCCTTCTTC CAGCGTGGCT TCTATTTGCA TCCCTTCTAC AGTCCTATCC 
CCTCCCATGC CCACAGAAGC ACTGGCTACA CCGGGTTACT TTCCTACAGT GGTGCAGCCT 
TTTGTGGAAT CTAACCTTTT GGTTCCTGTG GGCAGTATAG GAGGACAGGT TCAAGTGTCC 
CAGCCAGCAG TGAGTTTAGC ACAACAAGCC CCCACTACAT CCTCCCAGCA AGCAGCTCTG 
GAGAGCACTC AGGGAGTCTC TCAAGTTGCT CCTCCAGAGC CAGTTCCAGT GGCACAGGCA 
CAGCCTACCA CTTTGGTCTC CTCTATAGAC AGTGCACATT CAGATGTTGC TTCAGGAATG 
AGTGATGGCA ATGAGAATGT CCCGTCCTCC AGTGGAAGGC ATGAAGGGAG AGCTACAAAA 
CGACATTACC GAAAATCTGT AAGAAGTCGC TCTCGTCATG AAAAGACTTC GCGTCCAAAA 
TTAAGAATTT TGAATGTTTC AAATAAAGGA GACCGAGTAG TAGAATGTCA ACTAGAGACT 
CATAATCGGA AAATGGTTAC ATTCAAATTT GATTTAGATG GTGACAATCC TGAGGAGATA 
GCAACAATTA TGGTGAACAA TGACTTTATT CTAGCAATGG AGAGAGAGTC CTTTGTGGAT 
CAAGTACGAG AAATTATTGA AAAGGCTGAT GAAATGCTCA GTGAGGATGT CAGTGTGGAA 
CCAGAGGGTG ACCAGGGATT GGAGAGTCTG CAGGGAAAGG ATGACTATGG CTTTACAGGT 
TCTCAAAAAT TGGAAGGAGA ATTCAAACAA CCAGTTCCTG CGTCTTCCAT GCCACAGCAA 
ATAGGCATTC CTACCAGTTC TTTAACTCAA GTTGTTCATT CTGCTGGAAG ACGGTTTATA 
GTGAGTCCTG TGCCAGAAAG TCGATTACGA GAATCAAAAG TTTTCACTAG TGAAATACCA 
GATACAGTTG CTGCCTCTAC TCACAGCGCT GGAATGAACT TGTCTCACTC TGCTTCATCC 
CTTAGCCTAC AGCAAGCCTT TTCTGAACTT AGACATGTGC AGATGACAGA AGGGCCCAGC 
ACAGCACCTC CCAACTTCAG TCATACAGGA CCAACATTTC CAGTTGTTCC TCCTTCCATG 
AGTAGCATTG CTGGAGTCCC AACCACAGCA GTAGCCACAC CTTCAGTATC AGTCCCTGCA 
ACTAGCAGCC CTCTAAATGA CAGTTCCACA TCAGTAATTC AATCTGAGAT TCCAGTGCCC 
ACTGAAACAG GGGTTGGTGG AGTTCCCACC TGCACAAGTG TAATGCCTTC AAGTGGTCTC 
CCTGTACCAC CCATATCTGA ATCGCCAATA TTTTCCACTA TGGTTTCAAG CATCACAGTA 
CCTACAGTTG TCTCAATATC AGCAACATCC CAGCCAGTTC AAGCTCCCAC ATCTGGGAGC 
ACTGTTTCTA GCATAGGAAC TTTGCCTTCT ATACCAGTTT CAACAGCTTT AGCCTCTGCA 
GTGGGCAGTG TTGCTATCCC AGGTGCTAAG CCTCCACCTG TACTATCACA GCAGGCAGCA 
GGCAGTACTA CTGGGGCAGC CACATTAACA TCCGTTCCTG CCATCACTCC GTTCCCAAGC 
ATAGCTTCAC AGCCATCTCT TCAGCTTAGC AGCAGCACCT CTGCTCCAAC TCTAGCTGAA 
ACAGTGGTGG TTAGTGCACA CTCACTAGAT AAAACATCTC ATAGCAGTAC AACTGGATTG 
GCTTTGTCCC TGTCTGCATC ATCTTCCTCC CCTGGAGCAG GATTATCTAG TTCTATTTCT 
CAGCCTGGTG GGGTGCATCC TTTGGTTACC CCATCAGCTA TGGCTTCTAT TCCTGCCCTT 
CCTCAAACAG CAGGACCTAC TTCTACACCT TTATTGCCCC AAGTACTTAG TATCCCACCC 
TTGGTACAAC CTGTTGCCAG TGTGCCTGCT GTGCAGCAGA CACTAATTCA TAGTCAGCCT 
CAACCAGCTT TACTTCCCAA CCAGCCCCAC ACTCACTGTC CTGAAATAGA TGCTGACTTA 
CAGCCCAAAG CTCCTGGAAT TGATGACATA AAGACTCTAG AGGAAAAGCT GCGGTCTCTC 
TTTAGTGAAC CGAGCTCATC TGGAGCCCAA CATACATCGG TCTCACTAGA GACTTCACTG 
GTAGTAGAGA CCACTGTCAC GCCAGGTATC CCAACCACTG CTATTGCACC AAGCAAGCTC 
ATGACATCTA CTACAAGTAC ATGCTTACCA CCAACCAGTT TGCAATTAGG AACATCTGGT 
TTGTCAGTTA TACCAGTGGT CACGCCTGGG CAGGTTTCTA CCCCAGTCAG CTATGTGTCA 
GCCCCAGGCA GCACTACATC AGGAGTGAAA GCTGGAACTG CTCCATCAAA GCCACCTTTG 
ACTAAAGCTC CAGTGCTGCC AGTGGGTACT GAACTTCCAG CTGGTACTCC ACCCAGCGAG 
CAGCTGCCAC CTTTTCCAGG ACCTTCACTA ACTCAGTCCC AGCAACCTCT GGAAGATCTT 
GATGCTCAGT TGAGAAGAAC ACTTAGTCCA GAGACTATTG CGGTGACATC TACAATTGGT 
CCTGTGTCCA TGGTGGCTCC AACAGCAGTC ACAGAAGCAG GAGCACAGCC TCAGAAGGAT 
GTTTCTCAGA TCACAGAAGG TCCTGTCCTA GCGACTAGTT CGGGAACTGG TGTTTTTAAG 
ATGGGAAGAT TTCAGGTTTC AGTTGCAATG GATGATGTTC AAAAGGAGGG TAAAAATAAG 
TTAGAAGATG GAAAGTCTGT TCATTTTGAA TCCAGCACTT CAGAGTCCTC AGTGCTATCA 
AGTAGTAGTC CAGAAAGTAC CCTGGTGAAG ACAGAGCCTA ATGGGATAAC CATCCCTGGC 
ATCTCATCAG ATATGCCAGA TAGTGCCCAC AAAACTCCTG CCTCAGAAGC AAAGTCAGAA 
ACTGGGCAGC CTACCAAGGT TGGACGTTTT CAGGTGACAA CTACAGCAAA CAAAGTAGGT 
CGTTTCTCTG TGTCAAGAAC TGAAGACAAG CTCATTGAGG CAAAGAAAGA GGGACCAATA 
GCGTCTCCTC CTTTTATGGA TTTGGAACAA GGTGTTCTTC CAGCTGTGAT ACCAAAGAAA 
GAAAAGCCTG AACTGTCAGA GCCTTCACAT CTGAACGGAC CATCTTCTGA CCTGGAGGCT 
GCCTTCCTGA GCAGGGATGT GGATGATGGT TCAGGTAGTC CACACTCCCC CCATCAGCTG 
AGCTCAAAGA GCCTGCCTAT CCAGAATCTA AGTCAAAGCC TTAGTAATTC ATTCAACTCC 
TCTTACATGA GTAGTGACAA TGAGTCAGAT ATTGAAGATG AAGATTTAAA ATTAGAGCTG 
CGAAGACTAC GAGAAAAACA TCTTAAAGAG ATTCAAGACC TGCAGAGTCG CCAGAAGCAT 
GAAATTGAAT CTTTGTATAC CAAACTGGGA AAGGTTCCCC CTGCTGTCAT TATCCCCCCA 
GCTGCCCCCC TTTCAGGGAG AAGAAGGCGA CCCACTAAAA GCAAAGGCAG CAAGTCTAGT 
CGCAGCAGTT CCTTGGGGAA TAAAAGTCCC CAGCTTTCAG GTAACCTGTC TGGTCAGAGT 
GCTGCTTCAG TTTTGCACGC TCAGCAGACC CTCCACCCTC CTGGCAACAT CCCTGAGACT 
GGGCCAAATC AGCTGTTACA GCCCCTTAAG CCATCTCCCT CCAGTGACAA CCTCTACTCA 
GCCTTTACCA GTGATGGTGC CATTTCAGTC CCAAGCCTTT CTGCTCCAGG TCAAGGGACC 
AGCAGCACAA ACACTGTCGG GGGAACAGTG AACAGCCAAG CTGCCCCAGC TCAGCCTCCT 
GCCATGACAT CCAGCAGGAA GGGCACATTC ACAGATGACC TACACAAGTT GGTAGACAAT 
TGGGCCCGAG ATGCCATGAA TCTCTCAGGC AGAAGAGGAA GCAAAGGACA CATGAATTAT 
GAGGGCCCTG GAATGGCAAG AAAGTTCTCC GCACCTGGTC AGCTGTGCAT CTCCATGACC 
TCAAATCTGG GTGGTTCTGC CCCCATCTCT GCAGCATCAG CTACCTCTCT AGGTCACTTC 
ACCAAGTCTA TGTGCCCCCC ACAGCAGTAC GGTTTTCCAG CTCCCCCCTT TGGCACTCAG 
TGGAGTGGGA CGGGTGGCCC GGCACCACAG CCACTTGGCC AGTTCCAGCC TGTGGGAACT 
GCCTCCTTAC AGAATTTCAA CATCAGCAAT TTGCAGAAAT CCATCAGCAA CCCCCCAGGT 
TCCAACCTGC GGACCACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003433530.1
CDS	997..8937
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVPQSMAHPCGGTPTYPESQIFFPTIHERPVSFSPPPTCPPKVAISQRRKSTSFLEAQTRHFQPLLRTVGQNLLPPGGSPTNWTPEAVVMLGTTASRITGEPCEIQVHPMFEPTQVHSDYRPGLVLPEEAHYFIPQEAMYVAYQAQVTEQYEGIPYNSPVPSSPMKQIPEQKPVQGGPTSSSVFEFPSGQAFLVGHLQNLRLGSGLSPGSPLSSISAPISTDATCLKFHPVFVPHSAPAVLTHNNESRSNCVFEFHVHTPSSSSGEGGGILPQRVYRNRQVAVDLNQEEPPPQSVGLHGRLQPVTEEQRNYHAPDLTISVVEPIGQTWPIGSPEYSSDSSQITSSDPSDFQSPPPTGGAAVPFGSDVSLPFIHLPQTVLQESPLFFCFPQGTTSQQVLSASFSSGGSALHPQAQGQSQGQPSSSSLTGIPSSLPIQHSQQQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQGFPSRLPPQYPGDSNIAPSSSVASICIPSTVLSPPMPTEALATPGYFPTVVQPFVESNLLVPVGSIGGQVQVSQPAVSLAQQAPTTSSQQAALESTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_003433482.4
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003433482.4

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGCCT 
CAGTCCATGG CGCATCCGTG TGGGGGGACC CCAACATACC CAGAATCACA GATATTTTTC 
CCAACTATTC ATGAACGTCC AGTTTCTTTT TCACCACCTC CCACCTGTCC ACCGAAGGTG 
GCCATTTCCC AGCGACGTAA GAGCACCTCC TTCTTGGAAG CCCAAACTCG CCATTTCCAA 
CCCCTGCTGA GGACTGTTGG CCAAAATCTT CTTCCACCTG GTGGCAGCCC AACTAACTGG 
ACACCAGAGG CTGTAGTTAT GTTGGGCACT ACAGCCAGTA GAATAACTGG AGAGCCATGT 
GAGATACAGG TCCATCCTAT GTTTGAACCA ACTCAAGTTC ACAGTGACTA TAGACCTGGA 
CTGGTACTTC CAGAAGAAGC TCACTATTTT ATTCCTCAGG AAGCAATGTA TGTAGCTTAC 
CAGGCCCAGG TGACAGAACA GTATGAGGGT ATTCCATACA ACTCACCAGT ACCGTCAAGT 
CCTATGAAAC AGATACCTGA ACAGAAGCCA GTACAAGGGG GCCCTACTTC AAGTTCTGTC 
TTTGAATTTC CATCTGGACA GGCTTTCCTG GTAGGACACC TTCAAAATCT AAGGTTAGGT 
TCTGGATTGA GTCCAGGATC TCCCCTCTCT AGTATTTCTG CACCTATCAG TACAGATGCT 
ACATGTTTGA AATTTCACCC TGTCTTTGTT CCTCATTCTG CGCCTGCTGT GTTAACTCAT 
AACAATGAGA GCAGAAGCAA TTGTGTATTT GAGTTTCATG TTCATACTCC AAGCTCCTCT 
TCAGGAGAAG GAGGAGGAAT TTTACCTCAG CGTGTTTACC GAAATCGGCA GGTTGCAGTG 
GACTTGAATC AGGAAGAACC ACCTCCTCAA TCAGTTGGAT TACACGGCCG CCTACAGCCT 
GTGACTGAAG AACAGCGTAA TTACCATGCC CCAGATTTGA CCATTTCTGT GGTAGAGCCT 
ATCGGACAGA CCTGGCCAAT AGGAAGCCCA GAATACTCCA GTGATTCCTC ACAAATTACT 
TCTTCAGACC CCAGTGATTT TCAATCACCT CCCCCTACAG GGGGAGCAGC TGTACCATTT 
GGCTCTGACG TCTCATTACC CTTTATCCAT CTGCCTCAGA CAGTGTTACA AGAATCCCCA 
CTTTTCTTCT GTTTCCCCCA AGGAACCACA TCTCAGCAGG TTTTATCTGC CTCATTTTCT 
TCAGGAGGAT CTGCACTCCA TCCACAGGCA CAGGGGCAAA GCCAGGGTCA GCCATCCTCA 
AGTAGCTTAA CAGGGATTCC ATCTTCCCTC CCCATACAAC ATTCTCAGCA GCAGCAGGGA 
GTACAGCAGA CAGCCCCTCC ACAACAGACA GCGCAGTATT CGCTTCCACA GACATCAGCC 
TCCAGTGAGG CCACTATTGC ACAACCAGTC AGTCAGTCTC AGGCTCCACA GGTCTTGCCT 
CAAATATCAG CTGGAAAACA GGGCTTTCCA TCTCGACTGC CACCACAGTA CCCAGGAGAT 
TCAAATATTG CTCCTTCTTC CAGCGTGGCT TCTATTTGCA TCCCTTCTAC AGTCCTATCC 
CCTCCCATGC CCACAGAAGC ACTGGCTACA CCGGGTTACT TTCCTACAGT GGTGCAGCCT 
TTTGTGGAAT CTAACCTTTT GGTTCCTGTG GGCAGTATAG GAGGACAGGT TCAAGTGTCC 
CAGCCAGCAG TGAGTTTAGC ACAACAAGCC CCCACTACAT CCTCCCAGCA AGCAGCTCTG 
GAGAGCACTC AGGGAGTCTC TCAAGTTGCT CCTCCAGAGC CAGTTCCAGT GGCACAGGCA 
CAGCCTACCA CTTTGGTCTC CTCTATAGAC AGTGCACATT CAGATGTTGC TTCAGGAATG 
AGTGATGGCA ATGAGAATGT CCCGTCCTCC AGTGGAAGGC ATGAAGGGAG AGCTACAAAA 
CGACATTACC GAAAATCTGT AAGAAGTCGC TCTCGTCATG AAAAGACTTC GCGTCCAAAA 
TTAAGAATTT TGAATGTTTC AAATAAAGGA GACCGAGTAG TAGAATGTCA ACTAGAGACT 
CATAATCGGA AAATGGTTAC ATTCAAATTT GATTTAGATG GTGACAATCC TGAGGAGATA 
GCAACAATTA TGGTGAACAA TGACTTTATT CTAGCAATGG AGAGAGAGTC CTTTGTGGAT 
CAAGTACGAG AAATTATTGA AAAGGCTGAT GAAATGCTCA GTGAGGATGT CAGTGTGGAA 
CCAGAGGGTG ACCAGGGATT GGAGAGTCTG CAGGGAAAGG ATGACTATGG CTTTACAGGT 
TCTCAAAAAT TGGAAGGAGA ATTCAAACAA CCAGTTCCTG CGTCTTCCAT GCCACAGCAA 
ATAGGCATTC CTACCAGTTC TTTAACTCAA GTTGTTCATT CTGCTGGAAG ACGGTTTATA 
GTGAGTCCTG TGCCAGAAAG TCGATTACGA GAATCAAAAG TTTTCACTAG TGAAATACCA 
GATACAGTTG CTGCCTCTAC TCACAGCGCT GGAATGAACT TGTCTCACTC TGCTTCATCC 
CTTAGCCTAC AGCAAGCCTT TTCTGAACTT AGACATGTGC AGATGACAGA AGGGCCCAGC 
ACAGCACCTC CCAACTTCAG TCATACAGGA CCAACATTTC CAGTTGTTCC TCCTTCCATG 
AGTAGCATTG CTGGAGTCCC AACCACAGCA GTAGCCACAC CTTCAGTATC AGTCCCTGCA 
ACTAGCAGCC CTCTAAATGA CAGTTCCACA TCAGTAATTC AATCTGAGAT TCCAGTGCCC 
ACTGAAACAG GGGTTGGTGG AGTTCCCACC TGCACAAGTG TAATGCCTTC AAGTGGTCTC 
CCTGTACCAC CCATATCTGA ATCGCCAATA TTTTCCACTA TGGTTTCAAG CATCACAGTA 
CCTACAGTTG TCTCAATATC AGCAACATCC CAGCCAGTTC AAGCTCCCAC ATCTGGGAGC 
ACTGTTTCTA GCATAGGAAC TTTGCCTTCT ATACCAGTTT CAACAGCTTT AGCCTCTGCA 
GTGGGCAGTG TTGCTATCCC AGGTGCTAAG CCTCCACCTG TACTATCACA GCAGGCAGCA 
GGCAGTACTA CTGGGGCAGC CACATTAACA TCCGTTCCTG CCATCACTCC GTTCCCAAGC 
ATAGCTTCAC AGCCATCTCT TCAGCTTAGC AGCAGCACCT CTGCTCCAAC TCTAGCTGAA 
ACAGTGGTGG TTAGTGCACA CTCACTAGAT AAAACATCTC ATAGCAGTAC AACTGGATTG 
GCTTTGTCCC TGTCTGCATC ATCTTCCTCC CCTGGAGCAG GATTATCTAG TTCTATTTCT 
CAGCCTGGTG GGGTGCATCC TTTGGTTACC CCATCAGCTA TGGCTTCTAT TCCTGCCCTT 
CCTCAAACAG CAGGACCTAC TTCTACACCT TTATTGCCCC AAGTACTTAG TATCCCACCC 
TTGGTACAAC CTGTTGCCAG TGTGCCTGCT GTGCAGCAGA CACTAATTCA TAGTCAGCCT 
CAACCAGCTT TACTTCCCAA CCAGCCCCAC ACTCACTGTC CTGAAATAGA TGCTGACTTA 
CAGCCCAAAG CTCCTGGAAT TGATGACATA AAGACTCTAG AGGAAAAGCT GCGGTCTCTC 
TTTAGTGAAC CGAGCTCATC TGGAGCCCAA CATACATCGG TCTCACTAGA GACTTCACTG 
GTAGTAGAGA CCACTGTCAC GCCAGGTATC CCAACCACTG CTATTGCACC AAGCAAGCTC 
ATGACATCTA CTACAAGTAC ATGCTTACCA CCAACCAGTT TGCAATTAGG AACATCTGGT 
TTGTCAGTTA TACCAGTGGT CACGCCTGGG CAGGTTTCTA CCCCAGTCAG CTATGTGTCA 
GCCCCAGGCA GCACTACATC AGGAGTGAAA GCTGGAACTG CTCCATCAAA GCCACCTTTG 
ACTAAAGCTC CAGTGCTGCC AGTGGGTACT GAACTTCCAG CTGGTACTCC ACCCAGCGAG 
CAGCTGCCAC CTTTTCCAGG ACCTTCACTA ACTCAGTCCC AGCAACCTCT GGAAGATCTT 
GATGCTCAGT TGAGAAGAAC ACTTAGTCCA GAGACTATTG CGGTGACATC TACAATTGGT 
CCTGTGTCCA TGGTGGCTCC AACAGCAGTC ACAGAAGCAG GAGCACAGCC TCAGAAGGAT 
GTTTCTCAGA TCACAGAAGG TCCTGTCCTA GCGACTAGTT CGGGAACTGG TGTTTTTAAG 
ATGGGAAGAT TTCAGGTTTC AGTTGCAATG GATGATGTTC AAAAGGAGGG TAAAAATAAG 
TTAGAAGATG GAAAGTCTGT TCATTTTGAA TCCAGCACTT CAGAGTCCTC AGTGCTATCA 
AGTAGTAGTC CAGAAAGTAC CCTGGTGAAG ACAGAGCCTA ATGGGATAAC CATCCCTGGC 
ATCTCATCAG ATATGCCAGA TAGTGCCCAC AAAACTCCTG CCTCAGAAGC AAAGTCAGAA 
ACTGGGCAGC CTACCAAGGT TGGACGTTTT CAGGTGACAA CTACAGCAAA CAAAGTAGGT 
CGTTTCTCTG TGTCAAGAAC TGAAGACAAG CTCATTGAGG CAAAGAAAGA GGGACCAATA 
GCGTCTCCTC CTTTTATGGA TTTGGAACAA GGTGTTCTTC CAGCTGTGAT ACCAAAGAAA 
GAAAAGCCTG AACTGTCAGA GCCTTCACAT CTGAACGGAC CATCTTCTGA CCTGGAGGCT 
GCCTTCCTGA GCAGGGATGT GGATGATGGT TCAGGTAGTC CACACTCCCC CCATCAGCTG 
AGCTCAAAGA GCCTGCCTAT CCAGAATCTA AGTCAAAGCC TTAGTAATTC ATTCAACTCC 
TCTTACATGA GTAGTGACAA TGAGTCAGAT ATTGAAGATG AAGATTTAAA ATTAGAGCTG 
CGAAGACTAC GAGAAAAACA TCTTAAAGAG ATTCAAGACC TGCAGAGTCG CCAGAAGCAT 
GAAATTGAAT CTTTGTATAC CAAACTGGGA AAGGTTCCCC CTGCTGTCAT TATCCCCCCA 
GCTGCCCCCC TTTCAGGGAG AAGAAGGCGA CCCACTAAAA GCAAAGGCAG CAAGTCTAGT 
CGCAGCAGTT CCTTGGGGAA TAAAAGTCCC CAGCTTTCAG GTAACCTGTC TGGTCAGAGT 
GCTGCTTCAG TTTTGCACGC TCAGCAGACC CTCCACCCTC CTGGCAACAT CCCTGAGACT 
GGGCCAAATC AGCTGTTACA GCCCCTTAAG CCATCTCCCT CCAGTGACAA CCTCTACTCA 
GCCTTTACCA GTGATGGTGC CATTTCAGTC CCAAGCCTTT CTGCTCCAGG TCAAGGGACC 
AGCAGCACAA ACACTGTCGG GGGAACAGTG AACAGCCAAG CTGCCCCAGC TCAGCCTCCT 
GCCATGACAT CCAGCAGGAA GGGCACATTC ACAGATGACC TACACAAGTT GGTAGACAAT 
TGGGCCCGAG ATGCCATGAA TCTCTCAGGC AGAAGAGGAA GCAAAGGACA CATGAATTAT 
GAGGGCCCTG GAATGGCAAG AAAGTTCTCC GCACCTGGTC AGCTGTGCAT CTCCATGACC 
TCAAATCTGG GTGGTTCTGC CCCCATCTCT GCAGCATCAG CTACCTCTCT AGGTCACTTC 
ACCAAGTCTA TGTGCCCCCC ACAGCAGTAC GGTTTTCCAG CTCCCCCCTT TGGCACTCAG 
TGGAGTGGGA CGGGTGGCCC GGCACCACAG CCACTTGGCC AGTTCCAGCC TGTGGGAACT 
GCCTCCTTAC AGAATTTCAA CATCAGCAAT TTGCAGAAAT CCATCAGCAA CCCCCCAGGT 
TCCAACCTGC GGACCACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_003433482.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003433530.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X22, mRNA.

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CloneID	OCe27551
Clone ID Related Accession (Same CDS sequence)	XM_003433482.4 , XM_003433482.3
Accession Version	XM_003433482.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase WNK1 isoform X22
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003433482.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGCCT 
CAGTCCATGG CGCATCCGTG TGGGGGGACC CCAACATACC CAGAATCACA GATATTTTTC 
CCAACTATTC ATGAACGTCC AGTTTCTTTT TCACCACCTC CCACCTGTCC ACCGAAGGTG 
GCCATTTCCC AGCGACGTAA GAGCACCTCC TTCTTGGAAG CCCAAACTCG CCATTTCCAA 
CCCCTGCTGA GGACTGTTGG CCAAAATCTT CTTCCACCTG GTGGCAGCCC AACTAACTGG 
ACACCAGAGG CTGTAGTTAT GTTGGGCACT ACAGCCAGTA GAATAACTGG AGAGCCATGT 
GAGATACAGG TCCATCCTAT GTTTGAACCA ACTCAAGTTC ACAGTGACTA TAGACCTGGA 
CTGGTACTTC CAGAAGAAGC TCACTATTTT ATTCCTCAGG AAGCAATGTA TGTAGCTTAC 
CAGGCCCAGG TGACAGAACA GTATGAGGGT ATTCCATACA ACTCACCAGT ACCGTCAAGT 
CCTATGAAAC AGATACCTGA ACAGAAGCCA GTACAAGGGG GCCCTACTTC AAGTTCTGTC 
TTTGAATTTC CATCTGGACA GGCTTTCCTG GTAGGACACC TTCAAAATCT AAGGTTAGGT 
TCTGGATTGA GTCCAGGATC TCCCCTCTCT AGTATTTCTG CACCTATCAG TACAGATGCT 
ACATGTTTGA AATTTCACCC TGTCTTTGTT CCTCATTCTG CGCCTGCTGT GTTAACTCAT 
AACAATGAGA GCAGAAGCAA TTGTGTATTT GAGTTTCATG TTCATACTCC AAGCTCCTCT 
TCAGGAGAAG GAGGAGGAAT TTTACCTCAG CGTGTTTACC GAAATCGGCA GGTTGCAGTG 
GACTTGAATC AGGAAGAACC ACCTCCTCAA TCAGTTGGAT TACACGGCCG CCTACAGCCT 
GTGACTGAAG AACAGCGTAA TTACCATGCC CCAGATTTGA CCATTTCTGT GGTAGAGCCT 
ATCGGACAGA CCTGGCCAAT AGGAAGCCCA GAATACTCCA GTGATTCCTC ACAAATTACT 
TCTTCAGACC CCAGTGATTT TCAATCACCT CCCCCTACAG GGGGAGCAGC TGTACCATTT 
GGCTCTGACG TCTCATTACC CTTTATCCAT CTGCCTCAGA CAGTGTTACA AGAATCCCCA 
CTTTTCTTCT GTTTCCCCCA AGGAACCACA TCTCAGCAGG TTTTATCTGC CTCATTTTCT 
TCAGGAGGAT CTGCACTCCA TCCACAGGCA CAGGGGCAAA GCCAGGGTCA GCCATCCTCA 
AGTAGCTTAA CAGGGATTCC ATCTTCCCTC CCCATACAAC ATTCTCAGCA GCAGCAGGGA 
GTACAGCAGA CAGCCCCTCC ACAACAGACA GCGCAGTATT CGCTTCCACA GACATCAGCC 
TCCAGTGAGG CCACTATTGC ACAACCAGTC AGTCAGTCTC AGGCTCCACA GGTCTTGCCT 
CAAATATCAG CTGGAAAACA GGGCTTTCCA TCTCGACTGC CACCACAGTA CCCAGGAGAT 
TCAAATATTG CTCCTTCTTC CAGCGTGGCT TCTATTTGCA TCCCTTCTAC AGTCCTATCC 
CCTCCCATGC CCACAGAAGC ACTGGCTACA CCGGGTTACT TTCCTACAGT GGTGCAGCCT 
TTTGTGGAAT CTAACCTTTT GGTTCCTGTG GGCAGTATAG GAGGACAGGT TCAAGTGTCC 
CAGCCAGCAG TGAGTTTAGC ACAACAAGCC CCCACTACAT CCTCCCAGCA AGCAGCTCTG 
GAGAGCACTC AGGGAGTCTC TCAAGTTGCT CCTCCAGAGC CAGTTCCAGT GGCACAGGCA 
CAGCCTACCA CTTTGGTCTC CTCTATAGAC AGTGCACATT CAGATGTTGC TTCAGGAATG 
AGTGATGGCA ATGAGAATGT CCCGTCCTCC AGTGGAAGGC ATGAAGGGAG AGCTACAAAA 
CGACATTACC GAAAATCTGT AAGAAGTCGC TCTCGTCATG AAAAGACTTC GCGTCCAAAA 
TTAAGAATTT TGAATGTTTC AAATAAAGGA GACCGAGTAG TAGAATGTCA ACTAGAGACT 
CATAATCGGA AAATGGTTAC ATTCAAATTT GATTTAGATG GTGACAATCC TGAGGAGATA 
GCAACAATTA TGGTGAACAA TGACTTTATT CTAGCAATGG AGAGAGAGTC CTTTGTGGAT 
CAAGTACGAG AAATTATTGA AAAGGCTGAT GAAATGCTCA GTGAGGATGT CAGTGTGGAA 
CCAGAGGGTG ACCAGGGATT GGAGAGTCTG CAGGGAAAGG ATGACTATGG CTTTACAGGT 
TCTCAAAAAT TGGAAGGAGA ATTCAAACAA CCAGTTCCTG CGTCTTCCAT GCCACAGCAA 
ATAGGCATTC CTACCAGTTC TTTAACTCAA GTTGTTCATT CTGCTGGAAG ACGGTTTATA 
GTGAGTCCTG TGCCAGAAAG TCGATTACGA GAATCAAAAG TTTTCACTAG TGAAATACCA 
GATACAGTTG CTGCCTCTAC TCACAGCGCT GGAATGAACT TGTCTCACTC TGCTTCATCC 
CTTAGCCTAC AGCAAGCCTT TTCTGAACTT AGACATGTGC AGATGACAGA AGGGCCCAGC 
ACAGCACCTC CCAACTTCAG TCATACAGGA CCAACATTTC CAGTTGTTCC TCCTTCCATG 
AGTAGCATTG CTGGAGTCCC AACCACAGCA GTAGCCACAC CTTCAGTATC AGTCCCTGCA 
ACTAGCAGCC CTCTAAATGA CAGTTCCACA TCAGTAATTC AATCTGAGAT TCCAGTGCCC 
ACTGAAACAG GGGTTGGTGG AGTTCCCACC TGCACAAGTG TAATGCCTTC AAGTGGTCTC 
CCTGTACCAC CCATATCTGA ATCGCCAATA TTTTCCACTA TGGTTTCAAG CATCACAGTA 
CCTACAGTTG TCTCAATATC AGCAACATCC CAGCCAGTTC AAGCTCCCAC ATCTGGGAGC 
ACTGTTTCTA GCATAGGAAC TTTGCCTTCT ATACCAGTTT CAACAGCTTT AGCCTCTGCA 
GTGGGCAGTG TTGCTATCCC AGGTGCTAAG CCTCCACCTG TACTATCACA GCAGGCAGCA 
GGCAGTACTA CTGGGGCAGC CACATTAACA TCCGTTCCTG CCATCACTCC GTTCCCAAGC 
ATAGCTTCAC AGCCATCTCT TCAGCTTAGC AGCAGCACCT CTGCTCCAAC TCTAGCTGAA 
ACAGTGGTGG TTAGTGCACA CTCACTAGAT AAAACATCTC ATAGCAGTAC AACTGGATTG 
GCTTTGTCCC TGTCTGCATC ATCTTCCTCC CCTGGAGCAG GATTATCTAG TTCTATTTCT 
CAGCCTGGTG GGGTGCATCC TTTGGTTACC CCATCAGCTA TGGCTTCTAT TCCTGCCCTT 
CCTCAAACAG CAGGACCTAC TTCTACACCT TTATTGCCCC AAGTACTTAG TATCCCACCC 
TTGGTACAAC CTGTTGCCAG TGTGCCTGCT GTGCAGCAGA CACTAATTCA TAGTCAGCCT 
CAACCAGCTT TACTTCCCAA CCAGCCCCAC ACTCACTGTC CTGAAATAGA TGCTGACTTA 
CAGCCCAAAG CTCCTGGAAT TGATGACATA AAGACTCTAG AGGAAAAGCT GCGGTCTCTC 
TTTAGTGAAC CGAGCTCATC TGGAGCCCAA CATACATCGG TCTCACTAGA GACTTCACTG 
GTAGTAGAGA CCACTGTCAC GCCAGGTATC CCAACCACTG CTATTGCACC AAGCAAGCTC 
ATGACATCTA CTACAAGTAC ATGCTTACCA CCAACCAGTT TGCAATTAGG AACATCTGGT 
TTGTCAGTTA TACCAGTGGT CACGCCTGGG CAGGTTTCTA CCCCAGTCAG CTATGTGTCA 
GCCCCAGGCA GCACTACATC AGGAGTGAAA GCTGGAACTG CTCCATCAAA GCCACCTTTG 
ACTAAAGCTC CAGTGCTGCC AGTGGGTACT GAACTTCCAG CTGGTACTCC ACCCAGCGAG 
CAGCTGCCAC CTTTTCCAGG ACCTTCACTA ACTCAGTCCC AGCAACCTCT GGAAGATCTT 
GATGCTCAGT TGAGAAGAAC ACTTAGTCCA GAGACTATTG CGGTGACATC TACAATTGGT 
CCTGTGTCCA TGGTGGCTCC AACAGCAGTC ACAGAAGCAG GAGCACAGCC TCAGAAGGAT 
GTTTCTCAGA TCACAGAAGG TCCTGTCCTA GCGACTAGTT CGGGAACTGG TGTTTTTAAG 
ATGGGAAGAT TTCAGGTTTC AGTTGCAATG GATGATGTTC AAAAGGAGGG TAAAAATAAG 
TTAGAAGATG GAAAGTCTGT TCATTTTGAA TCCAGCACTT CAGAGTCCTC AGTGCTATCA 
AGTAGTAGTC CAGAAAGTAC CCTGGTGAAG ACAGAGCCTA ATGGGATAAC CATCCCTGGC 
ATCTCATCAG ATATGCCAGA TAGTGCCCAC AAAACTCCTG CCTCAGAAGC AAAGTCAGAA 
ACTGGGCAGC CTACCAAGGT TGGACGTTTT CAGGTGACAA CTACAGCAAA CAAAGTAGGT 
CGTTTCTCTG TGTCAAGAAC TGAAGACAAG CTCATTGAGG CAAAGAAAGA GGGACCAATA 
GCGTCTCCTC CTTTTATGGA TTTGGAACAA GGTGTTCTTC CAGCTGTGAT ACCAAAGAAA 
GAAAAGCCTG AACTGTCAGA GCCTTCACAT CTGAACGGAC CATCTTCTGA CCTGGAGGCT 
GCCTTCCTGA GCAGGGATGT GGATGATGGT TCAGGTAGTC CACACTCCCC CCATCAGCTG 
AGCTCAAAGA GCCTGCCTAT CCAGAATCTA AGTCAAAGCC TTAGTAATTC ATTCAACTCC 
TCTTACATGA GTAGTGACAA TGAGTCAGAT ATTGAAGATG AAGATTTAAA ATTAGAGCTG 
CGAAGACTAC GAGAAAAACA TCTTAAAGAG ATTCAAGACC TGCAGAGTCG CCAGAAGCAT 
GAAATTGAAT CTTTGTATAC CAAACTGGGA AAGGTTCCCC CTGCTGTCAT TATCCCCCCA 
GCTGCCCCCC TTTCAGGGAG AAGAAGGCGA CCCACTAAAA GCAAAGGCAG CAAGTCTAGT 
CGCAGCAGTT CCTTGGGGAA TAAAAGTCCC CAGCTTTCAG GTAACCTGTC TGGTCAGAGT 
GCTGCTTCAG TTTTGCACGC TCAGCAGACC CTCCACCCTC CTGGCAACAT CCCTGAGACT 
GGGCCAAATC AGCTGTTACA GCCCCTTAAG CCATCTCCCT CCAGTGACAA CCTCTACTCA 
GCCTTTACCA GTGATGGTGC CATTTCAGTC CCAAGCCTTT CTGCTCCAGG TCAAGGGACC 
AGCAGCACAA ACACTGTCGG GGGAACAGTG AACAGCCAAG CTGCCCCAGC TCAGCCTCCT 
GCCATGACAT CCAGCAGGAA GGGCACATTC ACAGATGACC TACACAAGTT GGTAGACAAT 
TGGGCCCGAG ATGCCATGAA TCTCTCAGGC AGAAGAGGAA GCAAAGGACA CATGAATTAT 
GAGGGCCCTG GAATGGCAAG AAAGTTCTCC GCACCTGGTC AGCTGTGCAT CTCCATGACC 
TCAAATCTGG GTGGTTCTGC CCCCATCTCT GCAGCATCAG CTACCTCTCT AGGTCACTTC 
ACCAAGTCTA TGTGCCCCCC ACAGCAGTAC GGTTTTCCAG CTCCCCCCTT TGGCACTCAG 
TGGAGTGGGA CGGGTGGCCC GGCACCACAG CCACTTGGCC AGTTCCAGCC TGTGGGAACT 
GCCTCCTTAC AGAATTTCAA CATCAGCAAT TTGCAGAAAT CCATCAGCAA CCCCCCAGGT 
TCCAACCTGC GGACCACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003433530.1
CDS	99..8039
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVPQSMAHPCGGTPTYPESQIFFPTIHERPVSFSPPPTCPPKVAISQRRKSTSFLEAQTRHFQPLLRTVGQNLLPPGGSPTNWTPEAVVMLGTTASRITGEPCEIQVHPMFEPTQVHSDYRPGLVLPEEAHYFIPQEAMYVAYQAQVTEQYEGIPYNSPVPSSPMKQIPEQKPVQGGPTSSSVFEFPSGQAFLVGHLQNLRLGSGLSPGSPLSSISAPISTDATCLKFHPVFVPHSAPAVLTHNNESRSNCVFEFHVHTPSSSSGEGGGILPQRVYRNRQVAVDLNQEEPPPQSVGLHGRLQPVTEEQRNYHAPDLTISVVEPIGQTWPIGSPEYSSDSSQITSSDPSDFQSPPPTGGAAVPFGSDVSLPFIHLPQTVLQESPLFFCFPQGTTSQQVLSASFSSGGSALHPQAQGQSQGQPSSSSLTGIPSSLPIQHSQQQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQGFPSRLPPQYPGDSNIAPSSSVASICIPSTVLSPPMPTEALATPGYFPTVVQPFVESNLLVPVGSIGGQVQVSQPAVSLAQQAPTTSSQQAALESTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_003433482.3
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003433482.3

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGCCT 
CAGTCCATGG CGCATCCGTG TGGGGGGACC CCAACATACC CAGAATCACA GATATTTTTC 
CCAACTATTC ATGAACGTCC AGTTTCTTTT TCACCACCTC CCACCTGTCC ACCGAAGGTG 
GCCATTTCCC AGCGACGTAA GAGCACCTCC TTCTTGGAAG CCCAAACTCG CCATTTCCAA 
CCCCTGCTGA GGACTGTTGG CCAAAATCTT CTTCCACCTG GTGGCAGCCC AACTAACTGG 
ACACCAGAGG CTGTAGTTAT GTTGGGCACT ACAGCCAGTA GAATAACTGG AGAGCCATGT 
GAGATACAGG TCCATCCTAT GTTTGAACCA ACTCAAGTTC ACAGTGACTA TAGACCTGGA 
CTGGTACTTC CAGAAGAAGC TCACTATTTT ATTCCTCAGG AAGCAATGTA TGTAGCTTAC 
CAGGCCCAGG TGACAGAACA GTATGAGGGT ATTCCATACA ACTCACCAGT ACCGTCAAGT 
CCTATGAAAC AGATACCTGA ACAGAAGCCA GTACAAGGGG GCCCTACTTC AAGTTCTGTC 
TTTGAATTTC CATCTGGACA GGCTTTCCTG GTAGGACACC TTCAAAATCT AAGGTTAGGT 
TCTGGATTGA GTCCAGGATC TCCCCTCTCT AGTATTTCTG CACCTATCAG TACAGATGCT 
ACATGTTTGA AATTTCACCC TGTCTTTGTT CCTCATTCTG CGCCTGCTGT GTTAACTCAT 
AACAATGAGA GCAGAAGCAA TTGTGTATTT GAGTTTCATG TTCATACTCC AAGCTCCTCT 
TCAGGAGAAG GAGGAGGAAT TTTACCTCAG CGTGTTTACC GAAATCGGCA GGTTGCAGTG 
GACTTGAATC AGGAAGAACC ACCTCCTCAA TCAGTTGGAT TACACGGCCG CCTACAGCCT 
GTGACTGAAG AACAGCGTAA TTACCATGCC CCAGATTTGA CCATTTCTGT GGTAGAGCCT 
ATCGGACAGA CCTGGCCAAT AGGAAGCCCA GAATACTCCA GTGATTCCTC ACAAATTACT 
TCTTCAGACC CCAGTGATTT TCAATCACCT CCCCCTACAG GGGGAGCAGC TGTACCATTT 
GGCTCTGACG TCTCATTACC CTTTATCCAT CTGCCTCAGA CAGTGTTACA AGAATCCCCA 
CTTTTCTTCT GTTTCCCCCA AGGAACCACA TCTCAGCAGG TTTTATCTGC CTCATTTTCT 
TCAGGAGGAT CTGCACTCCA TCCACAGGCA CAGGGGCAAA GCCAGGGTCA GCCATCCTCA 
AGTAGCTTAA CAGGGATTCC ATCTTCCCTC CCCATACAAC ATTCTCAGCA GCAGCAGGGA 
GTACAGCAGA CAGCCCCTCC ACAACAGACA GCGCAGTATT CGCTTCCACA GACATCAGCC 
TCCAGTGAGG CCACTATTGC ACAACCAGTC AGTCAGTCTC AGGCTCCACA GGTCTTGCCT 
CAAATATCAG CTGGAAAACA GGGCTTTCCA TCTCGACTGC CACCACAGTA CCCAGGAGAT 
TCAAATATTG CTCCTTCTTC CAGCGTGGCT TCTATTTGCA TCCCTTCTAC AGTCCTATCC 
CCTCCCATGC CCACAGAAGC ACTGGCTACA CCGGGTTACT TTCCTACAGT GGTGCAGCCT 
TTTGTGGAAT CTAACCTTTT GGTTCCTGTG GGCAGTATAG GAGGACAGGT TCAAGTGTCC 
CAGCCAGCAG TGAGTTTAGC ACAACAAGCC CCCACTACAT CCTCCCAGCA AGCAGCTCTG 
GAGAGCACTC AGGGAGTCTC TCAAGTTGCT CCTCCAGAGC CAGTTCCAGT GGCACAGGCA 
CAGCCTACCA CTTTGGTCTC CTCTATAGAC AGTGCACATT CAGATGTTGC TTCAGGAATG 
AGTGATGGCA ATGAGAATGT CCCGTCCTCC AGTGGAAGGC ATGAAGGGAG AGCTACAAAA 
CGACATTACC GAAAATCTGT AAGAAGTCGC TCTCGTCATG AAAAGACTTC GCGTCCAAAA 
TTAAGAATTT TGAATGTTTC AAATAAAGGA GACCGAGTAG TAGAATGTCA ACTAGAGACT 
CATAATCGGA AAATGGTTAC ATTCAAATTT GATTTAGATG GTGACAATCC TGAGGAGATA 
GCAACAATTA TGGTGAACAA TGACTTTATT CTAGCAATGG AGAGAGAGTC CTTTGTGGAT 
CAAGTACGAG AAATTATTGA AAAGGCTGAT GAAATGCTCA GTGAGGATGT CAGTGTGGAA 
CCAGAGGGTG ACCAGGGATT GGAGAGTCTG CAGGGAAAGG ATGACTATGG CTTTACAGGT 
TCTCAAAAAT TGGAAGGAGA ATTCAAACAA CCAGTTCCTG CGTCTTCCAT GCCACAGCAA 
ATAGGCATTC CTACCAGTTC TTTAACTCAA GTTGTTCATT CTGCTGGAAG ACGGTTTATA 
GTGAGTCCTG TGCCAGAAAG TCGATTACGA GAATCAAAAG TTTTCACTAG TGAAATACCA 
GATACAGTTG CTGCCTCTAC TCACAGCGCT GGAATGAACT TGTCTCACTC TGCTTCATCC 
CTTAGCCTAC AGCAAGCCTT TTCTGAACTT AGACATGTGC AGATGACAGA AGGGCCCAGC 
ACAGCACCTC CCAACTTCAG TCATACAGGA CCAACATTTC CAGTTGTTCC TCCTTCCATG 
AGTAGCATTG CTGGAGTCCC AACCACAGCA GTAGCCACAC CTTCAGTATC AGTCCCTGCA 
ACTAGCAGCC CTCTAAATGA CAGTTCCACA TCAGTAATTC AATCTGAGAT TCCAGTGCCC 
ACTGAAACAG GGGTTGGTGG AGTTCCCACC TGCACAAGTG TAATGCCTTC AAGTGGTCTC 
CCTGTACCAC CCATATCTGA ATCGCCAATA TTTTCCACTA TGGTTTCAAG CATCACAGTA 
CCTACAGTTG TCTCAATATC AGCAACATCC CAGCCAGTTC AAGCTCCCAC ATCTGGGAGC 
ACTGTTTCTA GCATAGGAAC TTTGCCTTCT ATACCAGTTT CAACAGCTTT AGCCTCTGCA 
GTGGGCAGTG TTGCTATCCC AGGTGCTAAG CCTCCACCTG TACTATCACA GCAGGCAGCA 
GGCAGTACTA CTGGGGCAGC CACATTAACA TCCGTTCCTG CCATCACTCC GTTCCCAAGC 
ATAGCTTCAC AGCCATCTCT TCAGCTTAGC AGCAGCACCT CTGCTCCAAC TCTAGCTGAA 
ACAGTGGTGG TTAGTGCACA CTCACTAGAT AAAACATCTC ATAGCAGTAC AACTGGATTG 
GCTTTGTCCC TGTCTGCATC ATCTTCCTCC CCTGGAGCAG GATTATCTAG TTCTATTTCT 
CAGCCTGGTG GGGTGCATCC TTTGGTTACC CCATCAGCTA TGGCTTCTAT TCCTGCCCTT 
CCTCAAACAG CAGGACCTAC TTCTACACCT TTATTGCCCC AAGTACTTAG TATCCCACCC 
TTGGTACAAC CTGTTGCCAG TGTGCCTGCT GTGCAGCAGA CACTAATTCA TAGTCAGCCT 
CAACCAGCTT TACTTCCCAA CCAGCCCCAC ACTCACTGTC CTGAAATAGA TGCTGACTTA 
CAGCCCAAAG CTCCTGGAAT TGATGACATA AAGACTCTAG AGGAAAAGCT GCGGTCTCTC 
TTTAGTGAAC CGAGCTCATC TGGAGCCCAA CATACATCGG TCTCACTAGA GACTTCACTG 
GTAGTAGAGA CCACTGTCAC GCCAGGTATC CCAACCACTG CTATTGCACC AAGCAAGCTC 
ATGACATCTA CTACAAGTAC ATGCTTACCA CCAACCAGTT TGCAATTAGG AACATCTGGT 
TTGTCAGTTA TACCAGTGGT CACGCCTGGG CAGGTTTCTA CCCCAGTCAG CTATGTGTCA 
GCCCCAGGCA GCACTACATC AGGAGTGAAA GCTGGAACTG CTCCATCAAA GCCACCTTTG 
ACTAAAGCTC CAGTGCTGCC AGTGGGTACT GAACTTCCAG CTGGTACTCC ACCCAGCGAG 
CAGCTGCCAC CTTTTCCAGG ACCTTCACTA ACTCAGTCCC AGCAACCTCT GGAAGATCTT 
GATGCTCAGT TGAGAAGAAC ACTTAGTCCA GAGACTATTG CGGTGACATC TACAATTGGT 
CCTGTGTCCA TGGTGGCTCC AACAGCAGTC ACAGAAGCAG GAGCACAGCC TCAGAAGGAT 
GTTTCTCAGA TCACAGAAGG TCCTGTCCTA GCGACTAGTT CGGGAACTGG TGTTTTTAAG 
ATGGGAAGAT TTCAGGTTTC AGTTGCAATG GATGATGTTC AAAAGGAGGG TAAAAATAAG 
TTAGAAGATG GAAAGTCTGT TCATTTTGAA TCCAGCACTT CAGAGTCCTC AGTGCTATCA 
AGTAGTAGTC CAGAAAGTAC CCTGGTGAAG ACAGAGCCTA ATGGGATAAC CATCCCTGGC 
ATCTCATCAG ATATGCCAGA TAGTGCCCAC AAAACTCCTG CCTCAGAAGC AAAGTCAGAA 
ACTGGGCAGC CTACCAAGGT TGGACGTTTT CAGGTGACAA CTACAGCAAA CAAAGTAGGT 
CGTTTCTCTG TGTCAAGAAC TGAAGACAAG CTCATTGAGG CAAAGAAAGA GGGACCAATA 
GCGTCTCCTC CTTTTATGGA TTTGGAACAA GGTGTTCTTC CAGCTGTGAT ACCAAAGAAA 
GAAAAGCCTG AACTGTCAGA GCCTTCACAT CTGAACGGAC CATCTTCTGA CCTGGAGGCT 
GCCTTCCTGA GCAGGGATGT GGATGATGGT TCAGGTAGTC CACACTCCCC CCATCAGCTG 
AGCTCAAAGA GCCTGCCTAT CCAGAATCTA AGTCAAAGCC TTAGTAATTC ATTCAACTCC 
TCTTACATGA GTAGTGACAA TGAGTCAGAT ATTGAAGATG AAGATTTAAA ATTAGAGCTG 
CGAAGACTAC GAGAAAAACA TCTTAAAGAG ATTCAAGACC TGCAGAGTCG CCAGAAGCAT 
GAAATTGAAT CTTTGTATAC CAAACTGGGA AAGGTTCCCC CTGCTGTCAT TATCCCCCCA 
GCTGCCCCCC TTTCAGGGAG AAGAAGGCGA CCCACTAAAA GCAAAGGCAG CAAGTCTAGT 
CGCAGCAGTT CCTTGGGGAA TAAAAGTCCC CAGCTTTCAG GTAACCTGTC TGGTCAGAGT 
GCTGCTTCAG TTTTGCACGC TCAGCAGACC CTCCACCCTC CTGGCAACAT CCCTGAGACT 
GGGCCAAATC AGCTGTTACA GCCCCTTAAG CCATCTCCCT CCAGTGACAA CCTCTACTCA 
GCCTTTACCA GTGATGGTGC CATTTCAGTC CCAAGCCTTT CTGCTCCAGG TCAAGGGACC 
AGCAGCACAA ACACTGTCGG GGGAACAGTG AACAGCCAAG CTGCCCCAGC TCAGCCTCCT 
GCCATGACAT CCAGCAGGAA GGGCACATTC ACAGATGACC TACACAAGTT GGTAGACAAT 
TGGGCCCGAG ATGCCATGAA TCTCTCAGGC AGAAGAGGAA GCAAAGGACA CATGAATTAT 
GAGGGCCCTG GAATGGCAAG AAAGTTCTCC GCACCTGGTC AGCTGTGCAT CTCCATGACC 
TCAAATCTGG GTGGTTCTGC CCCCATCTCT GCAGCATCAG CTACCTCTCT AGGTCACTTC 
ACCAAGTCTA TGTGCCCCCC ACAGCAGTAC GGTTTTCCAG CTCCCCCCTT TGGCACTCAG 
TGGAGTGGGA CGGGTGGCCC GGCACCACAG CCACTTGGCC AGTTCCAGCC TGTGGGAACT 
GCCTCCTTAC AGAATTTCAA CATCAGCAAT TTGCAGAAAT CCATCAGCAA CCCCCCAGGT 
TCCAACCTGC GGACCACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_005637356.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005637413.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA.

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CloneID	OCe27552
Clone ID Related Accession (Same CDS sequence)	XM_005637356.3 , XM_005637356.2
Accession Version	XM_005637356.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase WNK1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005637356.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG 
CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT 
CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC 
CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA 
GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG 
GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT 
CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA 
CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT 
CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA 
GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA 
TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG 
CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG 
TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT 
CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA 
AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG 
CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT 
GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA 
CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT 
AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA 
GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT 
CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG 
AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT 
ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT 
AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA 
GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT 
AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT 
AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT 
GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT 
GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT 
ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT 
GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG 
GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC 
AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA 
GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA 
GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT 
TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG 
CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT 
CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG 
GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG 
CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT 
AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA 
GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG 
ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG 
TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC 
CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT 
AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG 
CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT 
GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT 
GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT 
TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG 
GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA 
GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT 
AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC 
TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT 
GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT 
TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG 
TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA 
AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC 
TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC 
TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT 
TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA 
AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA 
ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT 
GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC 
AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT 
GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG 
CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC 
TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG 
AAGTTTAACT GTGCATCTGA GCAGGTGACA TTCAAACCTG GTGGCAGGAG GACCCGATTT 
CTGAGTACGC CCTGCTTGGC TCTTTGGAAG ATGGTGAAAA AAGTCTGTCC TTGCAACCAG 
CTCTGTAGGA CCAGCAGCAC AAACACTGTC GGGGGAACAG TGAACAGCCA AGCTGCCCCA 
GCTCAGCCTC CTGCCATGAC ATCCAGCAGG AAGGGCACAT TCACAGATGA CCTACACAAG 
TTGGTAGACA ATTGGGCCCG AGATGCCATG AATCTCTCAG GCAGAAGAGG AAGCAAAGGA 
CACATGAATT ATGAGGGCCC TGGAATGGCA AGAAAGTTCT CCGCACCTGG TCAGCTGTGC 
ATCTCCATGA CCTCAAATCT GGGTGGTTCT GCCCCCATCT CTGCAGCATC AGCTACCTCT 
CTAGGTCACT TCACCAAGTC TATGTGCCCC CCACAGCAGT ACGGTTTTCC AGCTCCCCCC 
TTTGGCACTC AGTGGAGTGG GACGGGTGGC CCGGCACCAC AGCCACTTGG CCAGTTCCAG 
CCTGTGGGAA CTGCCTCCTT ACAGAATTTC AACATCAGCA ATTTGCAGAA ATCCATCAGC 
AACCCCCCAG GTTCCAACCT GCGGACCACT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005637413.1
CDS	996..8348
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVGQSQGQPSSSSLTGIPSSLPIQHSQQQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQLPVSQPVPTIQGEPQIPVAAQPSVVPVHSGAHFLPVGQPLSTSLLPQYPVSQIPISTPHASAPQTGFSSLPITMAAGINQPLLTLASSATAAAIPGGPTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPSRLPPQYPGDSNIAPSSSVASICIPSTVLSPPMPTEALATPGYFPTVVQPFVESNLLVPVGSIGGQVQVSQPAVSLAQQAPTTSSQQAALEQNEMPMWGPCCHWLFCWESTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGCAKFNCASEQVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_005637356.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005637356.3

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG 
CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT 
CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC 
CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA 
GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG 
GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT 
CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA 
CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT 
CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA 
GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA 
TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG 
CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG 
TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT 
CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA 
AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG 
CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT 
GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA 
CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT 
AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA 
GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT 
CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG 
AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT 
ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT 
AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA 
GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT 
AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT 
AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT 
GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT 
GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT 
ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT 
GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG 
GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC 
AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA 
GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA 
GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT 
TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG 
CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT 
CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG 
GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG 
CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT 
AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA 
GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG 
ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG 
TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC 
CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT 
AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG 
CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT 
GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT 
GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT 
TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG 
GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA 
GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT 
AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC 
TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT 
GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT 
TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG 
TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA 
AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC 
TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC 
TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT 
TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA 
AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA 
ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT 
GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC 
AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT 
GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG 
CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC 
TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG 
AAGTTTAACT GTGCATCTGA GCAGGTGACA TTCAAACCTG GTGGCAGGAG GACCCGATTT 
CTGAGTACGC CCTGCTTGGC TCTTTGGAAG ATGGTGAAAA AAGTCTGTCC TTGCAACCAG 
CTCTGTAGGA CCAGCAGCAC AAACACTGTC GGGGGAACAG TGAACAGCCA AGCTGCCCCA 
GCTCAGCCTC CTGCCATGAC ATCCAGCAGG AAGGGCACAT TCACAGATGA CCTACACAAG 
TTGGTAGACA ATTGGGCCCG AGATGCCATG AATCTCTCAG GCAGAAGAGG AAGCAAAGGA 
CACATGAATT ATGAGGGCCC TGGAATGGCA AGAAAGTTCT CCGCACCTGG TCAGCTGTGC 
ATCTCCATGA CCTCAAATCT GGGTGGTTCT GCCCCCATCT CTGCAGCATC AGCTACCTCT 
CTAGGTCACT TCACCAAGTC TATGTGCCCC CCACAGCAGT ACGGTTTTCC AGCTCCCCCC 
TTTGGCACTC AGTGGAGTGG GACGGGTGGC CCGGCACCAC AGCCACTTGG CCAGTTCCAG 
CCTGTGGGAA CTGCCTCCTT ACAGAATTTC AACATCAGCA ATTTGCAGAA ATCCATCAGC 
AACCCCCCAG GTTCCAACCT GCGGACCACT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_005637356.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005637413.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X4, mRNA.

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CloneID	OCe27552
Clone ID Related Accession (Same CDS sequence)	XM_005637356.3 , XM_005637356.2
Accession Version	XM_005637356.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase WNK1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005637356.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG 
CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT 
CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC 
CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA 
GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG 
GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT 
CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA 
CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT 
CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA 
GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA 
TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG 
CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG 
TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT 
CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA 
AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG 
CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT 
GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA 
CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT 
AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA 
GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT 
CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG 
AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT 
ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT 
AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA 
GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT 
AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT 
AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT 
GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT 
GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT 
ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT 
GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG 
GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC 
AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA 
GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA 
GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT 
TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG 
CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT 
CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG 
GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG 
CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT 
AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA 
GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG 
ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG 
TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC 
CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT 
AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG 
CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT 
GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT 
GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT 
TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG 
GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA 
GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT 
AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC 
TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT 
GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT 
TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG 
TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA 
AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC 
TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC 
TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT 
TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA 
AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA 
ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT 
GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC 
AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT 
GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG 
CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC 
TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG 
AAGTTTAACT GTGCATCTGA GCAGGTGACA TTCAAACCTG GTGGCAGGAG GACCCGATTT 
CTGAGTACGC CCTGCTTGGC TCTTTGGAAG ATGGTGAAAA AAGTCTGTCC TTGCAACCAG 
CTCTGTAGGA CCAGCAGCAC AAACACTGTC GGGGGAACAG TGAACAGCCA AGCTGCCCCA 
GCTCAGCCTC CTGCCATGAC ATCCAGCAGG AAGGGCACAT TCACAGATGA CCTACACAAG 
TTGGTAGACA ATTGGGCCCG AGATGCCATG AATCTCTCAG GCAGAAGAGG AAGCAAAGGA 
CACATGAATT ATGAGGGCCC TGGAATGGCA AGAAAGTTCT CCGCACCTGG TCAGCTGTGC 
ATCTCCATGA CCTCAAATCT GGGTGGTTCT GCCCCCATCT CTGCAGCATC AGCTACCTCT 
CTAGGTCACT TCACCAAGTC TATGTGCCCC CCACAGCAGT ACGGTTTTCC AGCTCCCCCC 
TTTGGCACTC AGTGGAGTGG GACGGGTGGC CCGGCACCAC AGCCACTTGG CCAGTTCCAG 
CCTGTGGGAA CTGCCTCCTT ACAGAATTTC AACATCAGCA ATTTGCAGAA ATCCATCAGC 
AACCCCCCAG GTTCCAACCT GCGGACCACT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005637413.1
CDS	99..7451
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVGQSQGQPSSSSLTGIPSSLPIQHSQQQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQLPVSQPVPTIQGEPQIPVAAQPSVVPVHSGAHFLPVGQPLSTSLLPQYPVSQIPISTPHASAPQTGFSSLPITMAAGINQPLLTLASSATAAAIPGGPTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPSRLPPQYPGDSNIAPSSSVASICIPSTVLSPPMPTEALATPGYFPTVVQPFVESNLLVPVGSIGGQVQVSQPAVSLAQQAPTTSSQQAALEQNEMPMWGPCCHWLFCWESTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGCAKFNCASEQVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_005637356.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005637356.2

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG 
CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT 
CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC 
CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA 
GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG 
GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT 
CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA 
CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT 
CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA 
GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA 
TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG 
CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG 
TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT 
CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA 
AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG 
CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT 
GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA 
CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT 
AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA 
GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT 
CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG 
AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT 
ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT 
AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA 
GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT 
AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT 
AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT 
GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT 
GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT 
ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT 
GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG 
GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC 
AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA 
GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA 
GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT 
TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG 
CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT 
CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG 
GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG 
CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT 
AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA 
GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG 
ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG 
TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC 
CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT 
AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG 
CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT 
GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT 
GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT 
TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG 
GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA 
GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT 
AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC 
TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT 
GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT 
TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG 
TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA 
AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC 
TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC 
TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT 
TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA 
AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA 
ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT 
GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC 
AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT 
GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG 
CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC 
TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG 
AAGTTTAACT GTGCATCTGA GCAGGTGACA TTCAAACCTG GTGGCAGGAG GACCCGATTT 
CTGAGTACGC CCTGCTTGGC TCTTTGGAAG ATGGTGAAAA AAGTCTGTCC TTGCAACCAG 
CTCTGTAGGA CCAGCAGCAC AAACACTGTC GGGGGAACAG TGAACAGCCA AGCTGCCCCA 
GCTCAGCCTC CTGCCATGAC ATCCAGCAGG AAGGGCACAT TCACAGATGA CCTACACAAG 
TTGGTAGACA ATTGGGCCCG AGATGCCATG AATCTCTCAG GCAGAAGAGG AAGCAAAGGA 
CACATGAATT ATGAGGGCCC TGGAATGGCA AGAAAGTTCT CCGCACCTGG TCAGCTGTGC 
ATCTCCATGA CCTCAAATCT GGGTGGTTCT GCCCCCATCT CTGCAGCATC AGCTACCTCT 
CTAGGTCACT TCACCAAGTC TATGTGCCCC CCACAGCAGT ACGGTTTTCC AGCTCCCCCC 
TTTGGCACTC AGTGGAGTGG GACGGGTGGC CCGGCACCAC AGCCACTTGG CCAGTTCCAG 
CCTGTGGGAA CTGCCTCCTT ACAGAATTTC AACATCAGCA ATTTGCAGAA ATCCATCAGC 
AACCCCCCAG GTTCCAACCT GCGGACCACT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_022411000.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022266708.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X2, mRNA.

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CloneID	OCe27553
Clone ID Related Accession (Same CDS sequence)	XM_022411000.1
Accession Version	XM_022411000.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase WNK1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGGG AGTACAGCAG ACAGCCCCTC CACAACAGAC AGCGCAGTAT 
TCGCTTCCAC AGACATCAGC CTCCAGTGAG GCCACTATTG CACAACCAGT CAGTCAGTCT 
CAGGCTCCAC AGGTCTTGCC TCAAATATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA 
GTACCAACTA TCCAAGGCGA ACCTCAGATC CCAGTTGCGG CACAACCCTC AGTTGTTCCA 
GTCCACTCTG GTGCTCATTT CCTCCCTGTG GGACAGCCAC TCTCTACTTC CTTACTCCCT 
CAGTACCCGG TCTCTCAAAT TCCCATATCA ACTCCTCATG CGTCTGCGCC CCAGACAGGT 
TTTTCATCCC TTCCCATTAC AATGGCAGCT GGCATTAATC AGCCTCTGCT CACCTTGGCT 
TCATCTGCTA CAGCAGCTGC GATCCCAGGG GGACCAACTG TGGTTCCTAG TCAGCTTCCA 
ACCCTTCTGC AGCCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAACT CCTGCAACCA 
GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCTGAGGT TCCACTTCCC 
TCTGGAGATG TTCTGTACCA GGGCTTTCCA TCTCGACTGC CACCACAGTA CCCAGGAGAT 
TCAAATATTG CTCCTTCTTC CAGCGTGGCT TCTATTTGCA TCCCTTCTAC AGTCCTATCC 
CCTCCCATGC CCACAGAAGC ACTGGCTACA CCGGGTTACT TTCCTACAGT GGTGCAGCCT 
TTTGTGGAAT CTAACCTTTT GGTTCCTGTG GGCAGTATAG GAGGACAGGT TCAAGTGTCC 
CAGCCAGCAG TGAGTTTAGC ACAACAAGCC CCCACTACAT CCTCCCAGCA AGCAGCTCTG 
GAGCAGAATG AAATGCCAAT GTGGGGGCCT TGTTGCCACT GGCTGTTCTG TTGGGAAAGC 
ACTCAGGGAG TCTCTCAAGT TGCTCCTCCA GAGCCAGTTC CAGTGGCACA GGCACAGCCT 
ACCACTTTGG TCTCCTCTAT AGACAGTGCA CATTCAGATG TTGCTTCAGG AATGAGTGAT 
GGCAATGAGA ATGTCCCGTC CTCCAGTGGA AGGCATGAAG GGAGAGCTAC AAAACGACAT 
TACCGAAAAT CTGTAAGAAG TCGCTCTCGT CATGAAAAGA CTTCGCGTCC AAAATTAAGA 
ATTTTGAATG TTTCAAATAA AGGAGACCGA GTAGTAGAAT GTCAACTAGA GACTCATAAT 
CGGAAAATGG TTACATTCAA ATTTGATTTA GATGGTGACA ATCCTGAGGA GATAGCAACA 
ATTATGGTGA ACAATGACTT TATTCTAGCA ATGGAGAGAG AGTCCTTTGT GGATCAAGTA 
CGAGAAATTA TTGAAAAGGC TGATGAAATG CTCAGTGAGG ATGTCAGTGT GGAACCAGAG 
GGTGACCAGG GATTGGAGAG TCTGCAGGGA AAGGATGACT ATGGCTTTAC AGGTTCTCAA 
AAATTGGAAG GAGAATTCAA ACAACCAGTT CCTGCGTCTT CCATGCCACA GCAAATAGGC 
ATTCCTACCA GTTCTTTAAC TCAAGTTGTT CATTCTGCTG GAAGACGGTT TATAGTGAGT 
CCTGTGCCAG AAAGTCGATT ACGAGAATCA AAAGTTTTCA CTAGTGAAAT ACCAGATACA 
GTTGCTGCCT CTACTCACAG CGCTGGAATG AACTTGTCTC ACTCTGCTTC ATCCCTTAGC 
CTACAGCAAG CCTTTTCTGA ACTTAGACAT GTGCAGATGA CAGAAGGGCC CAGCACAGCA 
CCTCCCAACT TCAGTCATAC AGGACCAACA TTTCCAGTTG TTCCTCCTTC CATGAGTAGC 
ATTGCTGGAG TCCCAACCAC AGCAGTAGCC ACACCTTCAG TATCAGTCCC TGCAACTAGC 
AGCCCTCTAA ATGACAGTTC CACATCAGTA ATTCAATCTG AGATTCCAGT GCCCACTGAA 
ACAGGGGTTG GTGGAGTTCC CACCTGCACA AGTGTAATGC CTTCAAGTGG TCTCCCTGTA 
CCACCCATAT CTGAATCGCC AATATTTTCC ACTATGGTTT CAAGCATCAC AGTACCTACA 
GTTGTCTCAA TATCAGCAAC ATCCCAGCCA GTTCAAGCTC CCACATCTGG GAGCACTGTT 
TCTAGCATAG GAACTTTGCC TTCTATACCA GTTTCAACAG CTTTAGCCTC TGCAGTGGGC 
AGTGTTGCTA TCCCAGGTGC TAAGCCTCCA CCTGTACTAT CACAGCAGGC AGCAGGCAGT 
ACTACTGGGG CAGCCACATT AACATCCGTT CCTGCCATCA CTCCGTTCCC AAGCATAGCT 
TCACAGCCAT CTCTTCAGCT TAGCAGCAGC ACCTCTGCTC CAACTCTAGC TGAAACAGTG 
GTGGTTAGTG CACACTCACT AGATAAAACA TCTCATAGCA GTACAACTGG ATTGGCTTTG 
TCCCTGTCTG CATCATCTTC CTCCCCTGGA GCAGGATTAT CTAGTTCTAT TTCTCAGCCT 
GGTGGGGTGC ATCCTTTGGT TACCCCATCA GCTATGGCTT CTATTCCTGC CCTTCCTCAA 
ACAGCAGGAC CTACTTCTAC ACCTTTATTG CCCCAAGTAC TTAGTATCCC ACCCTTGGTA 
CAACCTGTTG CCAGTGTGCC TGCTGTGCAG CAGACACTAA TTCATAGTCA GCCTCAACCA 
GCTTTACTTC CCAACCAGCC CCACACTCAC TGTCCTGAAA TAGATGCTGA CTTACAGCCC 
AAAGCTCCTG GAATTGATGA CATAAAGACT CTAGAGGAAA AGCTGCGGTC TCTCTTTAGT 
GAACCGAGCT CATCTGGAGC CCAACATACA TCGGTCTCAC TAGAGACTTC ACTGGTAGTA 
GAGACCACTG TCACGCCAGG TATCCCAACC ACTGCTATTG CACCAAGCAA GCTCATGACA 
TCTACTACAA GTACATGCTT ACCACCAACC AGTTTGCAAT TAGGAACATC TGGTTTGTCA 
GTTATACCAG TGGTCACGCC TGGGCAGGTT TCTACCCCAG TCAGCTATGT GTCAGCCCCA 
GGCAGCACTA CATCAGGAGT GAAAGCTGGA ACTGCTCCAT CAAAGCCACC TTTGACTAAA 
GCTCCAGTGC TGCCAGTGGG TACTGAACTT CCAGCTGGTA CTCCACCCAG CGAGCAGCTG 
CCACCTTTTC CAGGACCTTC ACTAACTCAG TCCCAGCAAC CTCTGGAAGA TCTTGATGCT 
CAGTTGAGAA GAACACTTAG TCCAGAGACT ATTGCGGTGA CATCTACAAT TGGTCCTGTG 
TCCATGGTGG CTCCAACAGC AGTCACAGAA GCAGGAGCAC AGCCTCAGAA GGATGTTTCT 
CAGATCACAG AAGGTCCTGT CCTAGCGACT AGTTCGGGAA CTGGTGTTTT TAAGATGGGA 
AGATTTCAGG TTTCAGTTGC AATGGATGAT GTTCAAAAGG AGGGTAAAAA TAAGTTAGAA 
GATGGAAAGT CTGTTCATTT TGAATCCAGC ACTTCAGAGT CCTCAGTGCT ATCAAGTAGT 
AGTCCAGAAA GTACCCTGGT GAAGACAGAG CCTAATGGGA TAACCATCCC TGGCATCTCA 
TCAGATATGC CAGATAGTGC CCACAAAACT CCTGCCTCAG AAGCAAAGTC AGAAACTGGG 
CAGCCTACCA AGGTTGGACG TTTTCAGGTG ACAACTACAG CAAACAAAGT AGGTCGTTTC 
TCTGTGTCAA GAACTGAAGA CAAGCTCATT GAGGCAAAGA AAGAGGGACC AATAGCGTCT 
CCTCCTTTTA TGGATTTGGA ACAAGGTGTT CTTCCAGCTG TGATACCAAA GAAAGAAAAG 
CCTGAACTGT CAGAGCCTTC ACATCTGAAC GGACCATCTT CTGACCTGGA GGCTGCCTTC 
CTGAGCAGGG ATGTGGATGA TGGTTCAGGT AGTCCACACT CCCCCCATCA GCTGAGCTCA 
AAGAGCCTGC CTATCCAGAA TCTAAGTCAA AGCCTTAGTA ATTCATTCAA CTCCTCTTAC 
ATGAGTAGTG ACAATGAGTC AGATATTGAA GATGAAGATT TAAAATTAGA GCTGCGAAGA 
CTACGAGAAA AACATCTTAA AGAGATTCAA GACCTGCAGA GTCGCCAGAA GCATGAAATT 
GAATCTTTGT ATACCAAACT GGGAAAGGTT CCCCCTGCTG TCATTATCCC CCCAGCTGCC 
CCCCTTTCAG GGAGAAGAAG GCGACCCACT AAAAGCAAAG GCAGCAAGTC TAGTCGCAGC 
AGTTCCTTGG GGAATAAAAG TCCCCAGCTT TCAGGTAACC TGTCTGGTCA GAGTGCTGCT 
TCAGTTTTGC ACGCTCAGCA GACCCTCCAC CCTCCTGGCA ACATCCCTGA GACTGGGCCA 
AATCAGCTGT TACAGCCCCT TAAGCCATCT CCCTCCAGTG ACAACCTCTA CTCAGCCTTT 
ACCAGTGATG GTGCCATTTC AGTCCCAAGC CTTTCTGCTC CAGGTCAAGG CTGTGCGAAG 
TTTAACTGTG CATCTGAGCA GGTGACATTC AAACCTGGTG GCAGGAGGAC CCGATTTCTG 
AGTACGCCCT GCTTGGCTCT TTGGAAGATG GTGAAAAAAG TCTGTCCTTG CAACCAGCTC 
TGTAGGACCA GCAGCACAAA CACTGTCGGG GGAACAGTGA ACAGCCAAGC TGCCCCAGCT 
CAGCCTCCTG CCATGACATC CAGCAGGAAG GGCACATTCA CAGATGACCT ACACAAGTTG 
GTAGACAATT GGGCCCGAGA TGCCATGAAT CTCTCAGGCA GAAGAGGAAG CAAAGGACAC 
ATGAATTATG AGGGCCCTGG AATGGCAAGA AAGTTCTCCG CACCTGGTCA GCTGTGCATC 
TCCATGACCT CAAATCTGGG TGGTTCTGCC CCCATCTCTG CAGCATCAGC TACCTCTCTA 
GGTCACTTCA CCAAGTCTAT GTGCCCCCCA CAGCAGTACG GTTTTCCAGC TCCCCCCTTT 
GGCACTCAGT GGAGTGGGAC GGGTGGCCCG GCACCACAGC CACTTGGCCA GTTCCAGCCT 
GTGGGAACTG CCTCCTTACA GAATTTCAAC ATCAGCAATT TGCAGAAATC CATCAGCAAC 
CCCCCAGGTT CCAACCTGCG GACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022266708.1
CDS	996..8345
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVGQSQGQPSSSSLTGIPSSLPIQHSQQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQLPVSQPVPTIQGEPQIPVAAQPSVVPVHSGAHFLPVGQPLSTSLLPQYPVSQIPISTPHASAPQTGFSSLPITMAAGINQPLLTLASSATAAAIPGGPTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPSRLPPQYPGDSNIAPSSSVASICIPSTVLSPPMPTEALATPGYFPTVVQPFVESNLLVPVGSIGGQVQVSQPAVSLAQQAPTTSSQQAALEQNEMPMWGPCCHWLFCWESTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGCAKFNCASEQVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_022411000.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022411000.1

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGGG AGTACAGCAG ACAGCCCCTC CACAACAGAC AGCGCAGTAT 
TCGCTTCCAC AGACATCAGC CTCCAGTGAG GCCACTATTG CACAACCAGT CAGTCAGTCT 
CAGGCTCCAC AGGTCTTGCC TCAAATATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA 
GTACCAACTA TCCAAGGCGA ACCTCAGATC CCAGTTGCGG CACAACCCTC AGTTGTTCCA 
GTCCACTCTG GTGCTCATTT CCTCCCTGTG GGACAGCCAC TCTCTACTTC CTTACTCCCT 
CAGTACCCGG TCTCTCAAAT TCCCATATCA ACTCCTCATG CGTCTGCGCC CCAGACAGGT 
TTTTCATCCC TTCCCATTAC AATGGCAGCT GGCATTAATC AGCCTCTGCT CACCTTGGCT 
TCATCTGCTA CAGCAGCTGC GATCCCAGGG GGACCAACTG TGGTTCCTAG TCAGCTTCCA 
ACCCTTCTGC AGCCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAACT CCTGCAACCA 
GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCTGAGGT TCCACTTCCC 
TCTGGAGATG TTCTGTACCA GGGCTTTCCA TCTCGACTGC CACCACAGTA CCCAGGAGAT 
TCAAATATTG CTCCTTCTTC CAGCGTGGCT TCTATTTGCA TCCCTTCTAC AGTCCTATCC 
CCTCCCATGC CCACAGAAGC ACTGGCTACA CCGGGTTACT TTCCTACAGT GGTGCAGCCT 
TTTGTGGAAT CTAACCTTTT GGTTCCTGTG GGCAGTATAG GAGGACAGGT TCAAGTGTCC 
CAGCCAGCAG TGAGTTTAGC ACAACAAGCC CCCACTACAT CCTCCCAGCA AGCAGCTCTG 
GAGCAGAATG AAATGCCAAT GTGGGGGCCT TGTTGCCACT GGCTGTTCTG TTGGGAAAGC 
ACTCAGGGAG TCTCTCAAGT TGCTCCTCCA GAGCCAGTTC CAGTGGCACA GGCACAGCCT 
ACCACTTTGG TCTCCTCTAT AGACAGTGCA CATTCAGATG TTGCTTCAGG AATGAGTGAT 
GGCAATGAGA ATGTCCCGTC CTCCAGTGGA AGGCATGAAG GGAGAGCTAC AAAACGACAT 
TACCGAAAAT CTGTAAGAAG TCGCTCTCGT CATGAAAAGA CTTCGCGTCC AAAATTAAGA 
ATTTTGAATG TTTCAAATAA AGGAGACCGA GTAGTAGAAT GTCAACTAGA GACTCATAAT 
CGGAAAATGG TTACATTCAA ATTTGATTTA GATGGTGACA ATCCTGAGGA GATAGCAACA 
ATTATGGTGA ACAATGACTT TATTCTAGCA ATGGAGAGAG AGTCCTTTGT GGATCAAGTA 
CGAGAAATTA TTGAAAAGGC TGATGAAATG CTCAGTGAGG ATGTCAGTGT GGAACCAGAG 
GGTGACCAGG GATTGGAGAG TCTGCAGGGA AAGGATGACT ATGGCTTTAC AGGTTCTCAA 
AAATTGGAAG GAGAATTCAA ACAACCAGTT CCTGCGTCTT CCATGCCACA GCAAATAGGC 
ATTCCTACCA GTTCTTTAAC TCAAGTTGTT CATTCTGCTG GAAGACGGTT TATAGTGAGT 
CCTGTGCCAG AAAGTCGATT ACGAGAATCA AAAGTTTTCA CTAGTGAAAT ACCAGATACA 
GTTGCTGCCT CTACTCACAG CGCTGGAATG AACTTGTCTC ACTCTGCTTC ATCCCTTAGC 
CTACAGCAAG CCTTTTCTGA ACTTAGACAT GTGCAGATGA CAGAAGGGCC CAGCACAGCA 
CCTCCCAACT TCAGTCATAC AGGACCAACA TTTCCAGTTG TTCCTCCTTC CATGAGTAGC 
ATTGCTGGAG TCCCAACCAC AGCAGTAGCC ACACCTTCAG TATCAGTCCC TGCAACTAGC 
AGCCCTCTAA ATGACAGTTC CACATCAGTA ATTCAATCTG AGATTCCAGT GCCCACTGAA 
ACAGGGGTTG GTGGAGTTCC CACCTGCACA AGTGTAATGC CTTCAAGTGG TCTCCCTGTA 
CCACCCATAT CTGAATCGCC AATATTTTCC ACTATGGTTT CAAGCATCAC AGTACCTACA 
GTTGTCTCAA TATCAGCAAC ATCCCAGCCA GTTCAAGCTC CCACATCTGG GAGCACTGTT 
TCTAGCATAG GAACTTTGCC TTCTATACCA GTTTCAACAG CTTTAGCCTC TGCAGTGGGC 
AGTGTTGCTA TCCCAGGTGC TAAGCCTCCA CCTGTACTAT CACAGCAGGC AGCAGGCAGT 
ACTACTGGGG CAGCCACATT AACATCCGTT CCTGCCATCA CTCCGTTCCC AAGCATAGCT 
TCACAGCCAT CTCTTCAGCT TAGCAGCAGC ACCTCTGCTC CAACTCTAGC TGAAACAGTG 
GTGGTTAGTG CACACTCACT AGATAAAACA TCTCATAGCA GTACAACTGG ATTGGCTTTG 
TCCCTGTCTG CATCATCTTC CTCCCCTGGA GCAGGATTAT CTAGTTCTAT TTCTCAGCCT 
GGTGGGGTGC ATCCTTTGGT TACCCCATCA GCTATGGCTT CTATTCCTGC CCTTCCTCAA 
ACAGCAGGAC CTACTTCTAC ACCTTTATTG CCCCAAGTAC TTAGTATCCC ACCCTTGGTA 
CAACCTGTTG CCAGTGTGCC TGCTGTGCAG CAGACACTAA TTCATAGTCA GCCTCAACCA 
GCTTTACTTC CCAACCAGCC CCACACTCAC TGTCCTGAAA TAGATGCTGA CTTACAGCCC 
AAAGCTCCTG GAATTGATGA CATAAAGACT CTAGAGGAAA AGCTGCGGTC TCTCTTTAGT 
GAACCGAGCT CATCTGGAGC CCAACATACA TCGGTCTCAC TAGAGACTTC ACTGGTAGTA 
GAGACCACTG TCACGCCAGG TATCCCAACC ACTGCTATTG CACCAAGCAA GCTCATGACA 
TCTACTACAA GTACATGCTT ACCACCAACC AGTTTGCAAT TAGGAACATC TGGTTTGTCA 
GTTATACCAG TGGTCACGCC TGGGCAGGTT TCTACCCCAG TCAGCTATGT GTCAGCCCCA 
GGCAGCACTA CATCAGGAGT GAAAGCTGGA ACTGCTCCAT CAAAGCCACC TTTGACTAAA 
GCTCCAGTGC TGCCAGTGGG TACTGAACTT CCAGCTGGTA CTCCACCCAG CGAGCAGCTG 
CCACCTTTTC CAGGACCTTC ACTAACTCAG TCCCAGCAAC CTCTGGAAGA TCTTGATGCT 
CAGTTGAGAA GAACACTTAG TCCAGAGACT ATTGCGGTGA CATCTACAAT TGGTCCTGTG 
TCCATGGTGG CTCCAACAGC AGTCACAGAA GCAGGAGCAC AGCCTCAGAA GGATGTTTCT 
CAGATCACAG AAGGTCCTGT CCTAGCGACT AGTTCGGGAA CTGGTGTTTT TAAGATGGGA 
AGATTTCAGG TTTCAGTTGC AATGGATGAT GTTCAAAAGG AGGGTAAAAA TAAGTTAGAA 
GATGGAAAGT CTGTTCATTT TGAATCCAGC ACTTCAGAGT CCTCAGTGCT ATCAAGTAGT 
AGTCCAGAAA GTACCCTGGT GAAGACAGAG CCTAATGGGA TAACCATCCC TGGCATCTCA 
TCAGATATGC CAGATAGTGC CCACAAAACT CCTGCCTCAG AAGCAAAGTC AGAAACTGGG 
CAGCCTACCA AGGTTGGACG TTTTCAGGTG ACAACTACAG CAAACAAAGT AGGTCGTTTC 
TCTGTGTCAA GAACTGAAGA CAAGCTCATT GAGGCAAAGA AAGAGGGACC AATAGCGTCT 
CCTCCTTTTA TGGATTTGGA ACAAGGTGTT CTTCCAGCTG TGATACCAAA GAAAGAAAAG 
CCTGAACTGT CAGAGCCTTC ACATCTGAAC GGACCATCTT CTGACCTGGA GGCTGCCTTC 
CTGAGCAGGG ATGTGGATGA TGGTTCAGGT AGTCCACACT CCCCCCATCA GCTGAGCTCA 
AAGAGCCTGC CTATCCAGAA TCTAAGTCAA AGCCTTAGTA ATTCATTCAA CTCCTCTTAC 
ATGAGTAGTG ACAATGAGTC AGATATTGAA GATGAAGATT TAAAATTAGA GCTGCGAAGA 
CTACGAGAAA AACATCTTAA AGAGATTCAA GACCTGCAGA GTCGCCAGAA GCATGAAATT 
GAATCTTTGT ATACCAAACT GGGAAAGGTT CCCCCTGCTG TCATTATCCC CCCAGCTGCC 
CCCCTTTCAG GGAGAAGAAG GCGACCCACT AAAAGCAAAG GCAGCAAGTC TAGTCGCAGC 
AGTTCCTTGG GGAATAAAAG TCCCCAGCTT TCAGGTAACC TGTCTGGTCA GAGTGCTGCT 
TCAGTTTTGC ACGCTCAGCA GACCCTCCAC CCTCCTGGCA ACATCCCTGA GACTGGGCCA 
AATCAGCTGT TACAGCCCCT TAAGCCATCT CCCTCCAGTG ACAACCTCTA CTCAGCCTTT 
ACCAGTGATG GTGCCATTTC AGTCCCAAGC CTTTCTGCTC CAGGTCAAGG CTGTGCGAAG 
TTTAACTGTG CATCTGAGCA GGTGACATTC AAACCTGGTG GCAGGAGGAC CCGATTTCTG 
AGTACGCCCT GCTTGGCTCT TTGGAAGATG GTGAAAAAAG TCTGTCCTTG CAACCAGCTC 
TGTAGGACCA GCAGCACAAA CACTGTCGGG GGAACAGTGA ACAGCCAAGC TGCCCCAGCT 
CAGCCTCCTG CCATGACATC CAGCAGGAAG GGCACATTCA CAGATGACCT ACACAAGTTG 
GTAGACAATT GGGCCCGAGA TGCCATGAAT CTCTCAGGCA GAAGAGGAAG CAAAGGACAC 
ATGAATTATG AGGGCCCTGG AATGGCAAGA AAGTTCTCCG CACCTGGTCA GCTGTGCATC 
TCCATGACCT CAAATCTGGG TGGTTCTGCC CCCATCTCTG CAGCATCAGC TACCTCTCTA 
GGTCACTTCA CCAAGTCTAT GTGCCCCCCA CAGCAGTACG GTTTTCCAGC TCCCCCCTTT 
GGCACTCAGT GGAGTGGGAC GGGTGGCCCG GCACCACAGC CACTTGGCCA GTTCCAGCCT 
GTGGGAACTG CCTCCTTACA GAATTTCAAC ATCAGCAATT TGCAGAAATC CATCAGCAAC 
CCCCCAGGTT CCAACCTGCG GACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_022411003.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022266711.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X7, mRNA.

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CloneID	OCe27554
Clone ID Related Accession (Same CDS sequence)	XM_022411003.1
Accession Version	XM_022411003.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase WNK1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGCAG 
CAGGGAGTAC AGCAGACAGC CCCTCCACAA CAGACAGCGC AGTATTCGCT TCCACAGACA 
TCAGCCTCCA GTGAGGCCAC TATTGCACAA CCAGTCAGTC AGTCTCAGGC TCCACAGGTC 
TTGCCTCAAA TATCAGCTGG AAAACAGCTT CCAGTTTCCC AGCCAGTACC AACTATCCAA 
GGCGAACCTC AGATCCCAGT TGCGGCACAA CCCTCAGTTG TTCCAGTCCA CTCTGGTGCT 
CATTTCCTCC CTGTGGGACA GCCACTCTCT ACTTCCTTAC TCCCTCAGTA CCCGGTCTCT 
CAAATTCCCA TATCAACTCC TCATGCGTCT GCGCCCCAGA CAGGTTTTTC ATCCCTTCCC 
ATTACAATGG CAGCTGGCAT TAATCAGCCT CTGCTCACCT TGGCTTCATC TGCTACAGCA 
GCTGCGATCC CAGGGGGACC AACTGTGGTT CCTAGTCAGC TTCCAACCCT TCTGCAGCCT 
GTGACTCAGC TGCCAAGTCA GGTTCACCCA CAACTCCTGC AACCAGCAGT TCAGTCCATG 
GGAATACCAG CTAACCTTGG ACAAGCTGCT GAGGTTCCAC TTCCCTCTGG AGATGTTCTG 
TACCAGGGCT TTCCATCTCG ACTGCCACCA CAGTACCCAG GAGATTCAAA TATTGCTCCT 
TCTTCCAGCG TGGCTTCTAT TTGCATCCCT TCTACAGTCC TATCCCCTCC CATGCCCACA 
GAAGCACTGG CTACACCGGG TTACTTTCCT ACAGTGGTGC AGCCTTTTGT GGAATCTAAC 
CTTTTGGTTC CTGTGGGCAG TATAGGAGGA CAGGTTCAAG TGTCCCAGCC AGCAGTGAGT 
TTAGCACAAC AAGCCCCCAC TACATCCTCC CAGCAAGCAG CTCTGGAGCA GAATGAAATG 
CCAATGTGGG GGCCTTGTTG CCACTGGCTG TTCTGTTGGG AAAGCACTCA GGGAGTCTCT 
CAAGTTGCTC CTCCAGAGCC AGTTCCAGTG GCACAGGCAC AGCCTACCAC TTTGGTCTCC 
TCTATAGACA GTGCACATTC AGATGTTGCT TCAGGAATGA GTGATGGCAA TGAGAATGTC 
CCGTCCTCCA GTGGAAGGCA TGAAGGGAGA GCTACAAAAC GACATTACCG AAAATCTGTA 
AGAAGTCGCT CTCGTCATGA AAAGACTTCG CGTCCAAAAT TAAGAATTTT GAATGTTTCA 
AATAAAGGAG ACCGAGTAGT AGAATGTCAA CTAGAGACTC ATAATCGGAA AATGGTTACA 
TTCAAATTTG ATTTAGATGG TGACAATCCT GAGGAGATAG CAACAATTAT GGTGAACAAT 
GACTTTATTC TAGCAATGGA GAGAGAGTCC TTTGTGGATC AAGTACGAGA AATTATTGAA 
AAGGCTGATG AAATGCTCAG TGAGGATGTC AGTGTGGAAC CAGAGGGTGA CCAGGGATTG 
GAGAGTCTGC AGGGAAAGGA TGACTATGGC TTTACAGGTT CTCAAAAATT GGAAGGAGAA 
TTCAAACAAC CAGTTCCTGC GTCTTCCATG CCACAGCAAA TAGGCATTCC TACCAGTTCT 
TTAACTCAAG TTGTTCATTC TGCTGGAAGA CGGTTTATAG TGAGTCCTGT GCCAGAAAGT 
CGATTACGAG AATCAAAAGT TTTCACTAGT GAAATACCAG ATACAGTTGC TGCCTCTACT 
CACAGCGCTG GAATGAACTT GTCTCACTCT GCTTCATCCC TTAGCCTACA GCAAGCCTTT 
TCTGAACTTA GACATGTGCA GATGACAGAA GGGCCCAGCA CAGCACCTCC CAACTTCAGT 
CATACAGGAC CAACATTTCC AGTTGTTCCT CCTTCCATGA GTAGCATTGC TGGAGTCCCA 
ACCACAGCAG TAGCCACACC TTCAGTATCA GTCCCTGCAA CTAGCAGCCC TCTAAATGAC 
AGTTCCACAT CAGTAATTCA ATCTGAGATT CCAGTGCCCA CTGAAACAGG GGTTGGTGGA 
GTTCCCACCT GCACAAGTGT AATGCCTTCA AGTGGTCTCC CTGTACCACC CATATCTGAA 
TCGCCAATAT TTTCCACTAT GGTTTCAAGC ATCACAGTAC CTACAGTTGT CTCAATATCA 
GCAACATCCC AGCCAGTTCA AGCTCCCACA TCTGGGAGCA CTGTTTCTAG CATAGGAACT 
TTGCCTTCTA TACCAGTTTC AACAGCTTTA GCCTCTGCAG TGGGCAGTGT TGCTATCCCA 
GGTGCTAAGC CTCCACCTGT ACTATCACAG CAGGCAGCAG GCAGTACTAC TGGGGCAGCC 
ACATTAACAT CCGTTCCTGC CATCACTCCG TTCCCAAGCA TAGCTTCACA GCCATCTCTT 
CAGCTTAGCA GCAGCACCTC TGCTCCAACT CTAGCTGAAA CAGTGGTGGT TAGTGCACAC 
TCACTAGATA AAACATCTCA TAGCAGTACA ACTGGATTGG CTTTGTCCCT GTCTGCATCA 
TCTTCCTCCC CTGGAGCAGG ATTATCTAGT TCTATTTCTC AGCCTGGTGG GGTGCATCCT 
TTGGTTACCC CATCAGCTAT GGCTTCTATT CCTGCCCTTC CTCAAACAGC AGGACCTACT 
TCTACACCTT TATTGCCCCA AGTACTTAGT ATCCCACCCT TGGTACAACC TGTTGCCAGT 
GTGCCTGCTG TGCAGCAGAC ACTAATTCAT AGTCAGCCTC AACCAGCTTT ACTTCCCAAC 
CAGCCCCACA CTCACTGTCC TGAAATAGAT GCTGACTTAC AGCCCAAAGC TCCTGGAATT 
GATGACATAA AGACTCTAGA GGAAAAGCTG CGGTCTCTCT TTAGTGAACC GAGCTCATCT 
GGAGCCCAAC ATACATCGGT CTCACTAGAG ACTTCACTGG TAGTAGAGAC CACTGTCACG 
CCAGGTATCC CAACCACTGC TATTGCACCA AGCAAGCTCA TGACATCTAC TACAAGTACA 
TGCTTACCAC CAACCAGTTT GCAATTAGGA ACATCTGGTT TGTCAGTTAT ACCAGTGGTC 
ACGCCTGGGC AGGTTTCTAC CCCAGTCAGC TATGTGTCAG CCCCAGGCAG CACTACATCA 
GGAGTGAAAG CTGGAACTGC TCCATCAAAG CCACCTTTGA CTAAAGCTCC AGTGCTGCCA 
GTGGGTACTG AACTTCCAGC TGGTACTCCA CCCAGCGAGC AGCTGCCACC TTTTCCAGGA 
CCTTCACTAA CTCAGTCCCA GCAACCTCTG GAAGATCTTG ATGCTCAGTT GAGAAGAACA 
CTTAGTCCAG AGACTATTGC GGTGACATCT ACAATTGGTC CTGTGTCCAT GGTGGCTCCA 
ACAGCAGTCA CAGAAGCAGG AGCACAGCCT CAGAAGGATG TTTCTCAGAT CACAGAAGGT 
CCTGTCCTAG CGACTAGTTC GGGAACTGGT GTTTTTAAGA TGGGAAGATT TCAGGTTTCA 
GTTGCAATGG ATGATGTTCA AAAGGAGGGT AAAAATAAGT TAGAAGATGG AAAGTCTGTT 
CATTTTGAAT CCAGCACTTC AGAGTCCTCA GTGCTATCAA GTAGTAGTCC AGAAAGTACC 
CTGGTGAAGA CAGAGCCTAA TGGGATAACC ATCCCTGGCA TCTCATCAGA TATGCCAGAT 
AGTGCCCACA AAACTCCTGC CTCAGAAGCA AAGTCAGAAA CTGGGCAGCC TACCAAGGTT 
GGACGTTTTC AGGTGACAAC TACAGCAAAC AAAGTAGGTC GTTTCTCTGT GTCAAGAACT 
GAAGACAAGC TCATTGAGGC AAAGAAAGAG GGACCAATAG CGTCTCCTCC TTTTATGGAT 
TTGGAACAAG GTGTTCTTCC AGCTGTGATA CCAAAGAAAG AAAAGCCTGA ACTGTCAGAG 
CCTTCACATC TGAACGGACC ATCTTCTGAC CTGGAGGCTG CCTTCCTGAG CAGGGATGTG 
GATGATGGTT CAGGTAGTCC ACACTCCCCC CATCAGCTGA GCTCAAAGAG CCTGCCTATC 
CAGAATCTAA GTCAAAGCCT TAGTAATTCA TTCAACTCCT CTTACATGAG TAGTGACAAT 
GAGTCAGATA TTGAAGATGA AGATTTAAAA TTAGAGCTGC GAAGACTACG AGAAAAACAT 
CTTAAAGAGA TTCAAGACCT GCAGAGTCGC CAGAAGCATG AAATTGAATC TTTGTATACC 
AAACTGGGAA AGGTTCCCCC TGCTGTCATT ATCCCCCCAG CTGCCCCCCT TTCAGGGAGA 
AGAAGGCGAC CCACTAAAAG CAAAGGCAGC AAGTCTAGTC GCAGCAGTTC CTTGGGGAAT 
AAAAGTCCCC AGCTTTCAGG TAACCTGTCT GGTCAGAGTG CTGCTTCAGT TTTGCACGCT 
CAGCAGACCC TCCACCCTCC TGGCAACATC CCTGAGACTG GGCCAAATCA GCTGTTACAG 
CCCCTTAAGC CATCTCCCTC CAGTGACAAC CTCTACTCAG CCTTTACCAG TGATGGTGCC 
ATTTCAGTCC CAAGCCTTTC TGCTCCAGGT CAAGGCTGTG CGAAGTTTAA CTGTGCATCT 
GAGCAGGTGA CATTCAAACC TGGTGGCAGG AGGACCCGAT TTCTGAGTAC GCCCTGCTTG 
GCTCTTTGGA AGATGGTGAA AAAAGTCTGT CCTTGCAACC AGCTCTGTAG GACCAGCAGC 
ACAAACACTG TCGGGGGAAC AGTGAACAGC CAAGCTGCCC CAGCTCAGCC TCCTGCCATG 
ACATCCAGCA GGAAGGGCAC ATTCACAGAT GACCTACACA AGTTGGTAGA CAATTGGGCC 
CGAGATGCCA TGAATCTCTC AGGCAGAAGA GGAAGCAAAG GACACATGAA TTATGAGGGC 
CCTGGAATGG CAAGAAAGTT CTCCGCACCT GGTCAGCTGT GCATCTCCAT GACCTCAAAT 
CTGGGTGGTT CTGCCCCCAT CTCTGCAGCA TCAGCTACCT CTCTAGGTCA CTTCACCAAG 
TCTATGTGCC CCCCACAGCA GTACGGTTTT CCAGCTCCCC CCTTTGGCAC TCAGTGGAGT 
GGGACGGGTG GCCCGGCACC ACAGCCACTT GGCCAGTTCC AGCCTGTGGG AACTGCCTCC 
TTACAGAATT TCAACATCAG CAATTTGCAG AAATCCATCA GCAACCCCCC AGGTTCCAAC 
CTGCGGACCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022266711.1
CDS	996..8270
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQLPVSQPVPTIQGEPQIPVAAQPSVVPVHSGAHFLPVGQPLSTSLLPQYPVSQIPISTPHASAPQTGFSSLPITMAAGINQPLLTLASSATAAAIPGGPTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPSRLPPQYPGDSNIAPSSSVASICIPSTVLSPPMPTEALATPGYFPTVVQPFVESNLLVPVGSIGGQVQVSQPAVSLAQQAPTTSSQQAALEQNEMPMWGPCCHWLFCWESTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGCAKFNCASEQVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_022411003.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022411003.1

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGCAG 
CAGGGAGTAC AGCAGACAGC CCCTCCACAA CAGACAGCGC AGTATTCGCT TCCACAGACA 
TCAGCCTCCA GTGAGGCCAC TATTGCACAA CCAGTCAGTC AGTCTCAGGC TCCACAGGTC 
TTGCCTCAAA TATCAGCTGG AAAACAGCTT CCAGTTTCCC AGCCAGTACC AACTATCCAA 
GGCGAACCTC AGATCCCAGT TGCGGCACAA CCCTCAGTTG TTCCAGTCCA CTCTGGTGCT 
CATTTCCTCC CTGTGGGACA GCCACTCTCT ACTTCCTTAC TCCCTCAGTA CCCGGTCTCT 
CAAATTCCCA TATCAACTCC TCATGCGTCT GCGCCCCAGA CAGGTTTTTC ATCCCTTCCC 
ATTACAATGG CAGCTGGCAT TAATCAGCCT CTGCTCACCT TGGCTTCATC TGCTACAGCA 
GCTGCGATCC CAGGGGGACC AACTGTGGTT CCTAGTCAGC TTCCAACCCT TCTGCAGCCT 
GTGACTCAGC TGCCAAGTCA GGTTCACCCA CAACTCCTGC AACCAGCAGT TCAGTCCATG 
GGAATACCAG CTAACCTTGG ACAAGCTGCT GAGGTTCCAC TTCCCTCTGG AGATGTTCTG 
TACCAGGGCT TTCCATCTCG ACTGCCACCA CAGTACCCAG GAGATTCAAA TATTGCTCCT 
TCTTCCAGCG TGGCTTCTAT TTGCATCCCT TCTACAGTCC TATCCCCTCC CATGCCCACA 
GAAGCACTGG CTACACCGGG TTACTTTCCT ACAGTGGTGC AGCCTTTTGT GGAATCTAAC 
CTTTTGGTTC CTGTGGGCAG TATAGGAGGA CAGGTTCAAG TGTCCCAGCC AGCAGTGAGT 
TTAGCACAAC AAGCCCCCAC TACATCCTCC CAGCAAGCAG CTCTGGAGCA GAATGAAATG 
CCAATGTGGG GGCCTTGTTG CCACTGGCTG TTCTGTTGGG AAAGCACTCA GGGAGTCTCT 
CAAGTTGCTC CTCCAGAGCC AGTTCCAGTG GCACAGGCAC AGCCTACCAC TTTGGTCTCC 
TCTATAGACA GTGCACATTC AGATGTTGCT TCAGGAATGA GTGATGGCAA TGAGAATGTC 
CCGTCCTCCA GTGGAAGGCA TGAAGGGAGA GCTACAAAAC GACATTACCG AAAATCTGTA 
AGAAGTCGCT CTCGTCATGA AAAGACTTCG CGTCCAAAAT TAAGAATTTT GAATGTTTCA 
AATAAAGGAG ACCGAGTAGT AGAATGTCAA CTAGAGACTC ATAATCGGAA AATGGTTACA 
TTCAAATTTG ATTTAGATGG TGACAATCCT GAGGAGATAG CAACAATTAT GGTGAACAAT 
GACTTTATTC TAGCAATGGA GAGAGAGTCC TTTGTGGATC AAGTACGAGA AATTATTGAA 
AAGGCTGATG AAATGCTCAG TGAGGATGTC AGTGTGGAAC CAGAGGGTGA CCAGGGATTG 
GAGAGTCTGC AGGGAAAGGA TGACTATGGC TTTACAGGTT CTCAAAAATT GGAAGGAGAA 
TTCAAACAAC CAGTTCCTGC GTCTTCCATG CCACAGCAAA TAGGCATTCC TACCAGTTCT 
TTAACTCAAG TTGTTCATTC TGCTGGAAGA CGGTTTATAG TGAGTCCTGT GCCAGAAAGT 
CGATTACGAG AATCAAAAGT TTTCACTAGT GAAATACCAG ATACAGTTGC TGCCTCTACT 
CACAGCGCTG GAATGAACTT GTCTCACTCT GCTTCATCCC TTAGCCTACA GCAAGCCTTT 
TCTGAACTTA GACATGTGCA GATGACAGAA GGGCCCAGCA CAGCACCTCC CAACTTCAGT 
CATACAGGAC CAACATTTCC AGTTGTTCCT CCTTCCATGA GTAGCATTGC TGGAGTCCCA 
ACCACAGCAG TAGCCACACC TTCAGTATCA GTCCCTGCAA CTAGCAGCCC TCTAAATGAC 
AGTTCCACAT CAGTAATTCA ATCTGAGATT CCAGTGCCCA CTGAAACAGG GGTTGGTGGA 
GTTCCCACCT GCACAAGTGT AATGCCTTCA AGTGGTCTCC CTGTACCACC CATATCTGAA 
TCGCCAATAT TTTCCACTAT GGTTTCAAGC ATCACAGTAC CTACAGTTGT CTCAATATCA 
GCAACATCCC AGCCAGTTCA AGCTCCCACA TCTGGGAGCA CTGTTTCTAG CATAGGAACT 
TTGCCTTCTA TACCAGTTTC AACAGCTTTA GCCTCTGCAG TGGGCAGTGT TGCTATCCCA 
GGTGCTAAGC CTCCACCTGT ACTATCACAG CAGGCAGCAG GCAGTACTAC TGGGGCAGCC 
ACATTAACAT CCGTTCCTGC CATCACTCCG TTCCCAAGCA TAGCTTCACA GCCATCTCTT 
CAGCTTAGCA GCAGCACCTC TGCTCCAACT CTAGCTGAAA CAGTGGTGGT TAGTGCACAC 
TCACTAGATA AAACATCTCA TAGCAGTACA ACTGGATTGG CTTTGTCCCT GTCTGCATCA 
TCTTCCTCCC CTGGAGCAGG ATTATCTAGT TCTATTTCTC AGCCTGGTGG GGTGCATCCT 
TTGGTTACCC CATCAGCTAT GGCTTCTATT CCTGCCCTTC CTCAAACAGC AGGACCTACT 
TCTACACCTT TATTGCCCCA AGTACTTAGT ATCCCACCCT TGGTACAACC TGTTGCCAGT 
GTGCCTGCTG TGCAGCAGAC ACTAATTCAT AGTCAGCCTC AACCAGCTTT ACTTCCCAAC 
CAGCCCCACA CTCACTGTCC TGAAATAGAT GCTGACTTAC AGCCCAAAGC TCCTGGAATT 
GATGACATAA AGACTCTAGA GGAAAAGCTG CGGTCTCTCT TTAGTGAACC GAGCTCATCT 
GGAGCCCAAC ATACATCGGT CTCACTAGAG ACTTCACTGG TAGTAGAGAC CACTGTCACG 
CCAGGTATCC CAACCACTGC TATTGCACCA AGCAAGCTCA TGACATCTAC TACAAGTACA 
TGCTTACCAC CAACCAGTTT GCAATTAGGA ACATCTGGTT TGTCAGTTAT ACCAGTGGTC 
ACGCCTGGGC AGGTTTCTAC CCCAGTCAGC TATGTGTCAG CCCCAGGCAG CACTACATCA 
GGAGTGAAAG CTGGAACTGC TCCATCAAAG CCACCTTTGA CTAAAGCTCC AGTGCTGCCA 
GTGGGTACTG AACTTCCAGC TGGTACTCCA CCCAGCGAGC AGCTGCCACC TTTTCCAGGA 
CCTTCACTAA CTCAGTCCCA GCAACCTCTG GAAGATCTTG ATGCTCAGTT GAGAAGAACA 
CTTAGTCCAG AGACTATTGC GGTGACATCT ACAATTGGTC CTGTGTCCAT GGTGGCTCCA 
ACAGCAGTCA CAGAAGCAGG AGCACAGCCT CAGAAGGATG TTTCTCAGAT CACAGAAGGT 
CCTGTCCTAG CGACTAGTTC GGGAACTGGT GTTTTTAAGA TGGGAAGATT TCAGGTTTCA 
GTTGCAATGG ATGATGTTCA AAAGGAGGGT AAAAATAAGT TAGAAGATGG AAAGTCTGTT 
CATTTTGAAT CCAGCACTTC AGAGTCCTCA GTGCTATCAA GTAGTAGTCC AGAAAGTACC 
CTGGTGAAGA CAGAGCCTAA TGGGATAACC ATCCCTGGCA TCTCATCAGA TATGCCAGAT 
AGTGCCCACA AAACTCCTGC CTCAGAAGCA AAGTCAGAAA CTGGGCAGCC TACCAAGGTT 
GGACGTTTTC AGGTGACAAC TACAGCAAAC AAAGTAGGTC GTTTCTCTGT GTCAAGAACT 
GAAGACAAGC TCATTGAGGC AAAGAAAGAG GGACCAATAG CGTCTCCTCC TTTTATGGAT 
TTGGAACAAG GTGTTCTTCC AGCTGTGATA CCAAAGAAAG AAAAGCCTGA ACTGTCAGAG 
CCTTCACATC TGAACGGACC ATCTTCTGAC CTGGAGGCTG CCTTCCTGAG CAGGGATGTG 
GATGATGGTT CAGGTAGTCC ACACTCCCCC CATCAGCTGA GCTCAAAGAG CCTGCCTATC 
CAGAATCTAA GTCAAAGCCT TAGTAATTCA TTCAACTCCT CTTACATGAG TAGTGACAAT 
GAGTCAGATA TTGAAGATGA AGATTTAAAA TTAGAGCTGC GAAGACTACG AGAAAAACAT 
CTTAAAGAGA TTCAAGACCT GCAGAGTCGC CAGAAGCATG AAATTGAATC TTTGTATACC 
AAACTGGGAA AGGTTCCCCC TGCTGTCATT ATCCCCCCAG CTGCCCCCCT TTCAGGGAGA 
AGAAGGCGAC CCACTAAAAG CAAAGGCAGC AAGTCTAGTC GCAGCAGTTC CTTGGGGAAT 
AAAAGTCCCC AGCTTTCAGG TAACCTGTCT GGTCAGAGTG CTGCTTCAGT TTTGCACGCT 
CAGCAGACCC TCCACCCTCC TGGCAACATC CCTGAGACTG GGCCAAATCA GCTGTTACAG 
CCCCTTAAGC CATCTCCCTC CAGTGACAAC CTCTACTCAG CCTTTACCAG TGATGGTGCC 
ATTTCAGTCC CAAGCCTTTC TGCTCCAGGT CAAGGCTGTG CGAAGTTTAA CTGTGCATCT 
GAGCAGGTGA CATTCAAACC TGGTGGCAGG AGGACCCGAT TTCTGAGTAC GCCCTGCTTG 
GCTCTTTGGA AGATGGTGAA AAAAGTCTGT CCTTGCAACC AGCTCTGTAG GACCAGCAGC 
ACAAACACTG TCGGGGGAAC AGTGAACAGC CAAGCTGCCC CAGCTCAGCC TCCTGCCATG 
ACATCCAGCA GGAAGGGCAC ATTCACAGAT GACCTACACA AGTTGGTAGA CAATTGGGCC 
CGAGATGCCA TGAATCTCTC AGGCAGAAGA GGAAGCAAAG GACACATGAA TTATGAGGGC 
CCTGGAATGG CAAGAAAGTT CTCCGCACCT GGTCAGCTGT GCATCTCCAT GACCTCAAAT 
CTGGGTGGTT CTGCCCCCAT CTCTGCAGCA TCAGCTACCT CTCTAGGTCA CTTCACCAAG 
TCTATGTGCC CCCCACAGCA GTACGGTTTT CCAGCTCCCC CCTTTGGCAC TCAGTGGAGT 
GGGACGGGTG GCCCGGCACC ACAGCCACTT GGCCAGTTCC AGCCTGTGGG AACTGCCTCC 
TTACAGAATT TCAACATCAG CAATTTGCAG AAATCCATCA GCAACCCCCC AGGTTCCAAC 
CTGCGGACCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_022411004.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022266712.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X8, mRNA.

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CloneID	OCe27555
Clone ID Related Accession (Same CDS sequence)	XM_022411004.1
Accession Version	XM_022411004.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase WNK1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGCAG 
GGAGTACAGC AGACAGCCCC TCCACAACAG ACAGCGCAGT ATTCGCTTCC ACAGACATCA 
GCCTCCAGTG AGGCCACTAT TGCACAACCA GTCAGTCAGT CTCAGGCTCC ACAGGTCTTG 
CCTCAAATAT CAGCTGGAAA ACAGCTTCCA GTTTCCCAGC CAGTACCAAC TATCCAAGGC 
GAACCTCAGA TCCCAGTTGC GGCACAACCC TCAGTTGTTC CAGTCCACTC TGGTGCTCAT 
TTCCTCCCTG TGGGACAGCC ACTCTCTACT TCCTTACTCC CTCAGTACCC GGTCTCTCAA 
ATTCCCATAT CAACTCCTCA TGCGTCTGCG CCCCAGACAG GTTTTTCATC CCTTCCCATT 
ACAATGGCAG CTGGCATTAA TCAGCCTCTG CTCACCTTGG CTTCATCTGC TACAGCAGCT 
GCGATCCCAG GGGGACCAAC TGTGGTTCCT AGTCAGCTTC CAACCCTTCT GCAGCCTGTG 
ACTCAGCTGC CAAGTCAGGT TCACCCACAA CTCCTGCAAC CAGCAGTTCA GTCCATGGGA 
ATACCAGCTA ACCTTGGACA AGCTGCTGAG GTTCCACTTC CCTCTGGAGA TGTTCTGTAC 
CAGGGCTTTC CATCTCGACT GCCACCACAG TACCCAGGAG ATTCAAATAT TGCTCCTTCT 
TCCAGCGTGG CTTCTATTTG CATCCCTTCT ACAGTCCTAT CCCCTCCCAT GCCCACAGAA 
GCACTGGCTA CACCGGGTTA CTTTCCTACA GTGGTGCAGC CTTTTGTGGA ATCTAACCTT 
TTGGTTCCTG TGGGCAGTAT AGGAGGACAG GTTCAAGTGT CCCAGCCAGC AGTGAGTTTA 
GCACAACAAG CCCCCACTAC ATCCTCCCAG CAAGCAGCTC TGGAGCAGAA TGAAATGCCA 
ATGTGGGGGC CTTGTTGCCA CTGGCTGTTC TGTTGGGAAA GCACTCAGGG AGTCTCTCAA 
GTTGCTCCTC CAGAGCCAGT TCCAGTGGCA CAGGCACAGC CTACCACTTT GGTCTCCTCT 
ATAGACAGTG CACATTCAGA TGTTGCTTCA GGAATGAGTG ATGGCAATGA GAATGTCCCG 
TCCTCCAGTG GAAGGCATGA AGGGAGAGCT ACAAAACGAC ATTACCGAAA ATCTGTAAGA 
AGTCGCTCTC GTCATGAAAA GACTTCGCGT CCAAAATTAA GAATTTTGAA TGTTTCAAAT 
AAAGGAGACC GAGTAGTAGA ATGTCAACTA GAGACTCATA ATCGGAAAAT GGTTACATTC 
AAATTTGATT TAGATGGTGA CAATCCTGAG GAGATAGCAA CAATTATGGT GAACAATGAC 
TTTATTCTAG CAATGGAGAG AGAGTCCTTT GTGGATCAAG TACGAGAAAT TATTGAAAAG 
GCTGATGAAA TGCTCAGTGA GGATGTCAGT GTGGAACCAG AGGGTGACCA GGGATTGGAG 
AGTCTGCAGG GAAAGGATGA CTATGGCTTT ACAGGTTCTC AAAAATTGGA AGGAGAATTC 
AAACAACCAG TTCCTGCGTC TTCCATGCCA CAGCAAATAG GCATTCCTAC CAGTTCTTTA 
ACTCAAGTTG TTCATTCTGC TGGAAGACGG TTTATAGTGA GTCCTGTGCC AGAAAGTCGA 
TTACGAGAAT CAAAAGTTTT CACTAGTGAA ATACCAGATA CAGTTGCTGC CTCTACTCAC 
AGCGCTGGAA TGAACTTGTC TCACTCTGCT TCATCCCTTA GCCTACAGCA AGCCTTTTCT 
GAACTTAGAC ATGTGCAGAT GACAGAAGGG CCCAGCACAG CACCTCCCAA CTTCAGTCAT 
ACAGGACCAA CATTTCCAGT TGTTCCTCCT TCCATGAGTA GCATTGCTGG AGTCCCAACC 
ACAGCAGTAG CCACACCTTC AGTATCAGTC CCTGCAACTA GCAGCCCTCT AAATGACAGT 
TCCACATCAG TAATTCAATC TGAGATTCCA GTGCCCACTG AAACAGGGGT TGGTGGAGTT 
CCCACCTGCA CAAGTGTAAT GCCTTCAAGT GGTCTCCCTG TACCACCCAT ATCTGAATCG 
CCAATATTTT CCACTATGGT TTCAAGCATC ACAGTACCTA CAGTTGTCTC AATATCAGCA 
ACATCCCAGC CAGTTCAAGC TCCCACATCT GGGAGCACTG TTTCTAGCAT AGGAACTTTG 
CCTTCTATAC CAGTTTCAAC AGCTTTAGCC TCTGCAGTGG GCAGTGTTGC TATCCCAGGT 
GCTAAGCCTC CACCTGTACT ATCACAGCAG GCAGCAGGCA GTACTACTGG GGCAGCCACA 
TTAACATCCG TTCCTGCCAT CACTCCGTTC CCAAGCATAG CTTCACAGCC ATCTCTTCAG 
CTTAGCAGCA GCACCTCTGC TCCAACTCTA GCTGAAACAG TGGTGGTTAG TGCACACTCA 
CTAGATAAAA CATCTCATAG CAGTACAACT GGATTGGCTT TGTCCCTGTC TGCATCATCT 
TCCTCCCCTG GAGCAGGATT ATCTAGTTCT ATTTCTCAGC CTGGTGGGGT GCATCCTTTG 
GTTACCCCAT CAGCTATGGC TTCTATTCCT GCCCTTCCTC AAACAGCAGG ACCTACTTCT 
ACACCTTTAT TGCCCCAAGT ACTTAGTATC CCACCCTTGG TACAACCTGT TGCCAGTGTG 
CCTGCTGTGC AGCAGACACT AATTCATAGT CAGCCTCAAC CAGCTTTACT TCCCAACCAG 
CCCCACACTC ACTGTCCTGA AATAGATGCT GACTTACAGC CCAAAGCTCC TGGAATTGAT 
GACATAAAGA CTCTAGAGGA AAAGCTGCGG TCTCTCTTTA GTGAACCGAG CTCATCTGGA 
GCCCAACATA CATCGGTCTC ACTAGAGACT TCACTGGTAG TAGAGACCAC TGTCACGCCA 
GGTATCCCAA CCACTGCTAT TGCACCAAGC AAGCTCATGA CATCTACTAC AAGTACATGC 
TTACCACCAA CCAGTTTGCA ATTAGGAACA TCTGGTTTGT CAGTTATACC AGTGGTCACG 
CCTGGGCAGG TTTCTACCCC AGTCAGCTAT GTGTCAGCCC CAGGCAGCAC TACATCAGGA 
GTGAAAGCTG GAACTGCTCC ATCAAAGCCA CCTTTGACTA AAGCTCCAGT GCTGCCAGTG 
GGTACTGAAC TTCCAGCTGG TACTCCACCC AGCGAGCAGC TGCCACCTTT TCCAGGACCT 
TCACTAACTC AGTCCCAGCA ACCTCTGGAA GATCTTGATG CTCAGTTGAG AAGAACACTT 
AGTCCAGAGA CTATTGCGGT GACATCTACA ATTGGTCCTG TGTCCATGGT GGCTCCAACA 
GCAGTCACAG AAGCAGGAGC ACAGCCTCAG AAGGATGTTT CTCAGATCAC AGAAGGTCCT 
GTCCTAGCGA CTAGTTCGGG AACTGGTGTT TTTAAGATGG GAAGATTTCA GGTTTCAGTT 
GCAATGGATG ATGTTCAAAA GGAGGGTAAA AATAAGTTAG AAGATGGAAA GTCTGTTCAT 
TTTGAATCCA GCACTTCAGA GTCCTCAGTG CTATCAAGTA GTAGTCCAGA AAGTACCCTG 
GTGAAGACAG AGCCTAATGG GATAACCATC CCTGGCATCT CATCAGATAT GCCAGATAGT 
GCCCACAAAA CTCCTGCCTC AGAAGCAAAG TCAGAAACTG GGCAGCCTAC CAAGGTTGGA 
CGTTTTCAGG TGACAACTAC AGCAAACAAA GTAGGTCGTT TCTCTGTGTC AAGAACTGAA 
GACAAGCTCA TTGAGGCAAA GAAAGAGGGA CCAATAGCGT CTCCTCCTTT TATGGATTTG 
GAACAAGGTG TTCTTCCAGC TGTGATACCA AAGAAAGAAA AGCCTGAACT GTCAGAGCCT 
TCACATCTGA ACGGACCATC TTCTGACCTG GAGGCTGCCT TCCTGAGCAG GGATGTGGAT 
GATGGTTCAG GTAGTCCACA CTCCCCCCAT CAGCTGAGCT CAAAGAGCCT GCCTATCCAG 
AATCTAAGTC AAAGCCTTAG TAATTCATTC AACTCCTCTT ACATGAGTAG TGACAATGAG 
TCAGATATTG AAGATGAAGA TTTAAAATTA GAGCTGCGAA GACTACGAGA AAAACATCTT 
AAAGAGATTC AAGACCTGCA GAGTCGCCAG AAGCATGAAA TTGAATCTTT GTATACCAAA 
CTGGGAAAGG TTCCCCCTGC TGTCATTATC CCCCCAGCTG CCCCCCTTTC AGGGAGAAGA 
AGGCGACCCA CTAAAAGCAA AGGCAGCAAG TCTAGTCGCA GCAGTTCCTT GGGGAATAAA 
AGTCCCCAGC TTTCAGGTAA CCTGTCTGGT CAGAGTGCTG CTTCAGTTTT GCACGCTCAG 
CAGACCCTCC ACCCTCCTGG CAACATCCCT GAGACTGGGC CAAATCAGCT GTTACAGCCC 
CTTAAGCCAT CTCCCTCCAG TGACAACCTC TACTCAGCCT TTACCAGTGA TGGTGCCATT 
TCAGTCCCAA GCCTTTCTGC TCCAGGTCAA GGCTGTGCGA AGTTTAACTG TGCATCTGAG 
CAGGTGACAT TCAAACCTGG TGGCAGGAGG ACCCGATTTC TGAGTACGCC CTGCTTGGCT 
CTTTGGAAGA TGGTGAAAAA AGTCTGTCCT TGCAACCAGC TCTGTAGGAC CAGCAGCACA 
AACACTGTCG GGGGAACAGT GAACAGCCAA GCTGCCCCAG CTCAGCCTCC TGCCATGACA 
TCCAGCAGGA AGGGCACATT CACAGATGAC CTACACAAGT TGGTAGACAA TTGGGCCCGA 
GATGCCATGA ATCTCTCAGG CAGAAGAGGA AGCAAAGGAC ACATGAATTA TGAGGGCCCT 
GGAATGGCAA GAAAGTTCTC CGCACCTGGT CAGCTGTGCA TCTCCATGAC CTCAAATCTG 
GGTGGTTCTG CCCCCATCTC TGCAGCATCA GCTACCTCTC TAGGTCACTT CACCAAGTCT 
ATGTGCCCCC CACAGCAGTA CGGTTTTCCA GCTCCCCCCT TTGGCACTCA GTGGAGTGGG 
ACGGGTGGCC CGGCACCACA GCCACTTGGC CAGTTCCAGC CTGTGGGAAC TGCCTCCTTA 
CAGAATTTCA ACATCAGCAA TTTGCAGAAA TCCATCAGCA ACCCCCCAGG TTCCAACCTG 
CGGACCACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022266712.1
CDS	996..8267
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQLPVSQPVPTIQGEPQIPVAAQPSVVPVHSGAHFLPVGQPLSTSLLPQYPVSQIPISTPHASAPQTGFSSLPITMAAGINQPLLTLASSATAAAIPGGPTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPSRLPPQYPGDSNIAPSSSVASICIPSTVLSPPMPTEALATPGYFPTVVQPFVESNLLVPVGSIGGQVQVSQPAVSLAQQAPTTSSQQAALEQNEMPMWGPCCHWLFCWESTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGCAKFNCASEQVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_022411004.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022411004.1

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGCAG 
GGAGTACAGC AGACAGCCCC TCCACAACAG ACAGCGCAGT ATTCGCTTCC ACAGACATCA 
GCCTCCAGTG AGGCCACTAT TGCACAACCA GTCAGTCAGT CTCAGGCTCC ACAGGTCTTG 
CCTCAAATAT CAGCTGGAAA ACAGCTTCCA GTTTCCCAGC CAGTACCAAC TATCCAAGGC 
GAACCTCAGA TCCCAGTTGC GGCACAACCC TCAGTTGTTC CAGTCCACTC TGGTGCTCAT 
TTCCTCCCTG TGGGACAGCC ACTCTCTACT TCCTTACTCC CTCAGTACCC GGTCTCTCAA 
ATTCCCATAT CAACTCCTCA TGCGTCTGCG CCCCAGACAG GTTTTTCATC CCTTCCCATT 
ACAATGGCAG CTGGCATTAA TCAGCCTCTG CTCACCTTGG CTTCATCTGC TACAGCAGCT 
GCGATCCCAG GGGGACCAAC TGTGGTTCCT AGTCAGCTTC CAACCCTTCT GCAGCCTGTG 
ACTCAGCTGC CAAGTCAGGT TCACCCACAA CTCCTGCAAC CAGCAGTTCA GTCCATGGGA 
ATACCAGCTA ACCTTGGACA AGCTGCTGAG GTTCCACTTC CCTCTGGAGA TGTTCTGTAC 
CAGGGCTTTC CATCTCGACT GCCACCACAG TACCCAGGAG ATTCAAATAT TGCTCCTTCT 
TCCAGCGTGG CTTCTATTTG CATCCCTTCT ACAGTCCTAT CCCCTCCCAT GCCCACAGAA 
GCACTGGCTA CACCGGGTTA CTTTCCTACA GTGGTGCAGC CTTTTGTGGA ATCTAACCTT 
TTGGTTCCTG TGGGCAGTAT AGGAGGACAG GTTCAAGTGT CCCAGCCAGC AGTGAGTTTA 
GCACAACAAG CCCCCACTAC ATCCTCCCAG CAAGCAGCTC TGGAGCAGAA TGAAATGCCA 
ATGTGGGGGC CTTGTTGCCA CTGGCTGTTC TGTTGGGAAA GCACTCAGGG AGTCTCTCAA 
GTTGCTCCTC CAGAGCCAGT TCCAGTGGCA CAGGCACAGC CTACCACTTT GGTCTCCTCT 
ATAGACAGTG CACATTCAGA TGTTGCTTCA GGAATGAGTG ATGGCAATGA GAATGTCCCG 
TCCTCCAGTG GAAGGCATGA AGGGAGAGCT ACAAAACGAC ATTACCGAAA ATCTGTAAGA 
AGTCGCTCTC GTCATGAAAA GACTTCGCGT CCAAAATTAA GAATTTTGAA TGTTTCAAAT 
AAAGGAGACC GAGTAGTAGA ATGTCAACTA GAGACTCATA ATCGGAAAAT GGTTACATTC 
AAATTTGATT TAGATGGTGA CAATCCTGAG GAGATAGCAA CAATTATGGT GAACAATGAC 
TTTATTCTAG CAATGGAGAG AGAGTCCTTT GTGGATCAAG TACGAGAAAT TATTGAAAAG 
GCTGATGAAA TGCTCAGTGA GGATGTCAGT GTGGAACCAG AGGGTGACCA GGGATTGGAG 
AGTCTGCAGG GAAAGGATGA CTATGGCTTT ACAGGTTCTC AAAAATTGGA AGGAGAATTC 
AAACAACCAG TTCCTGCGTC TTCCATGCCA CAGCAAATAG GCATTCCTAC CAGTTCTTTA 
ACTCAAGTTG TTCATTCTGC TGGAAGACGG TTTATAGTGA GTCCTGTGCC AGAAAGTCGA 
TTACGAGAAT CAAAAGTTTT CACTAGTGAA ATACCAGATA CAGTTGCTGC CTCTACTCAC 
AGCGCTGGAA TGAACTTGTC TCACTCTGCT TCATCCCTTA GCCTACAGCA AGCCTTTTCT 
GAACTTAGAC ATGTGCAGAT GACAGAAGGG CCCAGCACAG CACCTCCCAA CTTCAGTCAT 
ACAGGACCAA CATTTCCAGT TGTTCCTCCT TCCATGAGTA GCATTGCTGG AGTCCCAACC 
ACAGCAGTAG CCACACCTTC AGTATCAGTC CCTGCAACTA GCAGCCCTCT AAATGACAGT 
TCCACATCAG TAATTCAATC TGAGATTCCA GTGCCCACTG AAACAGGGGT TGGTGGAGTT 
CCCACCTGCA CAAGTGTAAT GCCTTCAAGT GGTCTCCCTG TACCACCCAT ATCTGAATCG 
CCAATATTTT CCACTATGGT TTCAAGCATC ACAGTACCTA CAGTTGTCTC AATATCAGCA 
ACATCCCAGC CAGTTCAAGC TCCCACATCT GGGAGCACTG TTTCTAGCAT AGGAACTTTG 
CCTTCTATAC CAGTTTCAAC AGCTTTAGCC TCTGCAGTGG GCAGTGTTGC TATCCCAGGT 
GCTAAGCCTC CACCTGTACT ATCACAGCAG GCAGCAGGCA GTACTACTGG GGCAGCCACA 
TTAACATCCG TTCCTGCCAT CACTCCGTTC CCAAGCATAG CTTCACAGCC ATCTCTTCAG 
CTTAGCAGCA GCACCTCTGC TCCAACTCTA GCTGAAACAG TGGTGGTTAG TGCACACTCA 
CTAGATAAAA CATCTCATAG CAGTACAACT GGATTGGCTT TGTCCCTGTC TGCATCATCT 
TCCTCCCCTG GAGCAGGATT ATCTAGTTCT ATTTCTCAGC CTGGTGGGGT GCATCCTTTG 
GTTACCCCAT CAGCTATGGC TTCTATTCCT GCCCTTCCTC AAACAGCAGG ACCTACTTCT 
ACACCTTTAT TGCCCCAAGT ACTTAGTATC CCACCCTTGG TACAACCTGT TGCCAGTGTG 
CCTGCTGTGC AGCAGACACT AATTCATAGT CAGCCTCAAC CAGCTTTACT TCCCAACCAG 
CCCCACACTC ACTGTCCTGA AATAGATGCT GACTTACAGC CCAAAGCTCC TGGAATTGAT 
GACATAAAGA CTCTAGAGGA AAAGCTGCGG TCTCTCTTTA GTGAACCGAG CTCATCTGGA 
GCCCAACATA CATCGGTCTC ACTAGAGACT TCACTGGTAG TAGAGACCAC TGTCACGCCA 
GGTATCCCAA CCACTGCTAT TGCACCAAGC AAGCTCATGA CATCTACTAC AAGTACATGC 
TTACCACCAA CCAGTTTGCA ATTAGGAACA TCTGGTTTGT CAGTTATACC AGTGGTCACG 
CCTGGGCAGG TTTCTACCCC AGTCAGCTAT GTGTCAGCCC CAGGCAGCAC TACATCAGGA 
GTGAAAGCTG GAACTGCTCC ATCAAAGCCA CCTTTGACTA AAGCTCCAGT GCTGCCAGTG 
GGTACTGAAC TTCCAGCTGG TACTCCACCC AGCGAGCAGC TGCCACCTTT TCCAGGACCT 
TCACTAACTC AGTCCCAGCA ACCTCTGGAA GATCTTGATG CTCAGTTGAG AAGAACACTT 
AGTCCAGAGA CTATTGCGGT GACATCTACA ATTGGTCCTG TGTCCATGGT GGCTCCAACA 
GCAGTCACAG AAGCAGGAGC ACAGCCTCAG AAGGATGTTT CTCAGATCAC AGAAGGTCCT 
GTCCTAGCGA CTAGTTCGGG AACTGGTGTT TTTAAGATGG GAAGATTTCA GGTTTCAGTT 
GCAATGGATG ATGTTCAAAA GGAGGGTAAA AATAAGTTAG AAGATGGAAA GTCTGTTCAT 
TTTGAATCCA GCACTTCAGA GTCCTCAGTG CTATCAAGTA GTAGTCCAGA AAGTACCCTG 
GTGAAGACAG AGCCTAATGG GATAACCATC CCTGGCATCT CATCAGATAT GCCAGATAGT 
GCCCACAAAA CTCCTGCCTC AGAAGCAAAG TCAGAAACTG GGCAGCCTAC CAAGGTTGGA 
CGTTTTCAGG TGACAACTAC AGCAAACAAA GTAGGTCGTT TCTCTGTGTC AAGAACTGAA 
GACAAGCTCA TTGAGGCAAA GAAAGAGGGA CCAATAGCGT CTCCTCCTTT TATGGATTTG 
GAACAAGGTG TTCTTCCAGC TGTGATACCA AAGAAAGAAA AGCCTGAACT GTCAGAGCCT 
TCACATCTGA ACGGACCATC TTCTGACCTG GAGGCTGCCT TCCTGAGCAG GGATGTGGAT 
GATGGTTCAG GTAGTCCACA CTCCCCCCAT CAGCTGAGCT CAAAGAGCCT GCCTATCCAG 
AATCTAAGTC AAAGCCTTAG TAATTCATTC AACTCCTCTT ACATGAGTAG TGACAATGAG 
TCAGATATTG AAGATGAAGA TTTAAAATTA GAGCTGCGAA GACTACGAGA AAAACATCTT 
AAAGAGATTC AAGACCTGCA GAGTCGCCAG AAGCATGAAA TTGAATCTTT GTATACCAAA 
CTGGGAAAGG TTCCCCCTGC TGTCATTATC CCCCCAGCTG CCCCCCTTTC AGGGAGAAGA 
AGGCGACCCA CTAAAAGCAA AGGCAGCAAG TCTAGTCGCA GCAGTTCCTT GGGGAATAAA 
AGTCCCCAGC TTTCAGGTAA CCTGTCTGGT CAGAGTGCTG CTTCAGTTTT GCACGCTCAG 
CAGACCCTCC ACCCTCCTGG CAACATCCCT GAGACTGGGC CAAATCAGCT GTTACAGCCC 
CTTAAGCCAT CTCCCTCCAG TGACAACCTC TACTCAGCCT TTACCAGTGA TGGTGCCATT 
TCAGTCCCAA GCCTTTCTGC TCCAGGTCAA GGCTGTGCGA AGTTTAACTG TGCATCTGAG 
CAGGTGACAT TCAAACCTGG TGGCAGGAGG ACCCGATTTC TGAGTACGCC CTGCTTGGCT 
CTTTGGAAGA TGGTGAAAAA AGTCTGTCCT TGCAACCAGC TCTGTAGGAC CAGCAGCACA 
AACACTGTCG GGGGAACAGT GAACAGCCAA GCTGCCCCAG CTCAGCCTCC TGCCATGACA 
TCCAGCAGGA AGGGCACATT CACAGATGAC CTACACAAGT TGGTAGACAA TTGGGCCCGA 
GATGCCATGA ATCTCTCAGG CAGAAGAGGA AGCAAAGGAC ACATGAATTA TGAGGGCCCT 
GGAATGGCAA GAAAGTTCTC CGCACCTGGT CAGCTGTGCA TCTCCATGAC CTCAAATCTG 
GGTGGTTCTG CCCCCATCTC TGCAGCATCA GCTACCTCTC TAGGTCACTT CACCAAGTCT 
ATGTGCCCCC CACAGCAGTA CGGTTTTCCA GCTCCCCCCT TTGGCACTCA GTGGAGTGGG 
ACGGGTGGCC CGGCACCACA GCCACTTGGC CAGTTCCAGC CTGTGGGAAC TGCCTCCTTA 
CAGAATTTCA ACATCAGCAA TTTGCAGAAA TCCATCAGCA ACCCCCCAGG TTCCAACCTG 
CGGACCACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_022411006.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022266714.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X9, mRNA.

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CloneID	OCe27556
Clone ID Related Accession (Same CDS sequence)	XM_022411006.1
Accession Version	XM_022411006.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase WNK1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG 
CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT 
CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC 
CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA 
GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG 
GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT 
CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA 
CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT 
CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA 
GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA 
TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG 
CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG 
TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT 
CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA 
AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG 
CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT 
GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA 
CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT 
AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA 
GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT 
CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG 
AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT 
ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT 
AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA 
GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT 
AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT 
AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT 
GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT 
GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT 
ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT 
GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG 
GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC 
AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA 
GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA 
GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT 
TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG 
CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT 
CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG 
GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG 
CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT 
AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA 
GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG 
ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG 
TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC 
CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT 
AAAGCTCCAT CCCAGCAACC TCTGGAAGAT CTTGATGCTC AGTTGAGAAG AACACTTAGT 
CCAGAGACTA TTGCGGTGAC ATCTACAATT GGTCCTGTGT CCATGGTGGC TCCAACAGCA 
GTCACAGAAG CAGGAGCACA GCCTCAGAAG GATGTTTCTC AGATCACAGA AGGTCCTGTC 
CTAGCGACTA GTTCGGGAAC TGGTGTTTTT AAGATGGGAA GATTTCAGGT TTCAGTTGCA 
ATGGATGATG TTCAAAAGGA GGGTAAAAAT AAGTTAGAAG ATGGAAAGTC TGTTCATTTT 
GAATCCAGCA CTTCAGAGTC CTCAGTGCTA TCAAGTAGTA GTCCAGAAAG TACCCTGGTG 
AAGACAGAGC CTAATGGGAT AACCATCCCT GGCATCTCAT CAGATATGCC AGATAGTGCC 
CACAAAACTC CTGCCTCAGA AGCAAAGTCA GAAACTGGGC AGCCTACCAA GGTTGGACGT 
TTTCAGGTGA CAACTACAGC AAACAAAGTA GGTCGTTTCT CTGTGTCAAG AACTGAAGAC 
AAGCTCATTG AGGCAAAGAA AGAGGGACCA ATAGCGTCTC CTCCTTTTAT GGATTTGGAA 
CAAGGTGTTC TTCCAGCTGT GATACCAAAG AAAGAAAAGC CTGAACTGTC AGAGCCTTCA 
CATCTGAACG GACCATCTTC TGACCTGGAG GCTGCCTTCC TGAGCAGGGA TGTGGATGAT 
GGTTCAGGTA GTCCACACTC CCCCCATCAG CTGAGCTCAA AGAGCCTGCC TATCCAGAAT 
CTAAGTCAAA GCCTTAGTAA TTCATTCAAC TCCTCTTACA TGAGTAGTGA CAATGAGTCA 
GATATTGAAG ATGAAGATTT AAAATTAGAG CTGCGAAGAC TACGAGAAAA ACATCTTAAA 
GAGATTCAAG ACCTGCAGAG TCGCCAGAAG CATGAAATTG AATCTTTGTA TACCAAACTG 
GGAAAGGTTC CCCCTGCTGT CATTATCCCC CCAGCTGCCC CCCTTTCAGG GAGAAGAAGG 
CGACCCACTA AAAGCAAAGG CAGCAAGTCT AGTCGCAGCA GTTCCTTGGG GAATAAAAGT 
CCCCAGCTTT CAGGTAACCT GTCTGGTCAG AGTGCTGCTT CAGTTTTGCA CGCTCAGCAG 
ACCCTCCACC CTCCTGGCAA CATCCCTGAG ACTGGGCCAA ATCAGCTGTT ACAGCCCCTT 
AAGCCATCTC CCTCCAGTGA CAACCTCTAC TCAGCCTTTA CCAGTGATGG TGCCATTTCA 
GTCCCAAGCC TTTCTGCTCC AGGTCAAGGC TGTGCGAAGT TTAACTGTGC ATCTGAGCAG 
GTGACATTCA AACCTGGTGG CAGGAGGACC CGATTTCTGA GTACGCCCTG CTTGGCTCTT 
TGGAAGATGG TGAAAAAAGT CTGTCCTTGC AACCAGCTCT GTAGGACCAG CAGCACAAAC 
ACTGTCGGGG GAACAGTGAA CAGCCAAGCT GCCCCAGCTC AGCCTCCTGC CATGACATCC 
AGCAGGAAGG GCACATTCAC AGATGACCTA CACAAGTTGG TAGACAATTG GGCCCGAGAT 
GCCATGAATC TCTCAGGCAG AAGAGGAAGC AAAGGACACA TGAATTATGA GGGCCCTGGA 
ATGGCAAGAA AGTTCTCCGC ACCTGGTCAG CTGTGCATCT CCATGACCTC AAATCTGGGT 
GGTTCTGCCC CCATCTCTGC AGCATCAGCT ACCTCTCTAG GTCACTTCAC CAAGTCTATG 
TGCCCCCCAC AGCAGTACGG TTTTCCAGCT CCCCCCTTTG GCACTCAGTG GAGTGGGACG 
GGTGGCCCGG CACCACAGCC ACTTGGCCAG TTCCAGCCTG TGGGAACTGC CTCCTTACAG 
AATTTCAACA TCAGCAATTT GCAGAAATCC ATCAGCAACC CCCCAGGTTC CAACCTGCGG 
ACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022266714.1
CDS	996..8264
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVGQSQGQPSSSSLTGIPSSLPIQHSQQQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQLPVSQPVPTIQGEPQIPVAAQPSVVPVHSGAHFLPVGQPLSTSLLPQYPVSQIPISTPHASAPQTGFSSLPITMAAGINQPLLTLASSATAAAIPGGPTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPSRLPPQYPGDSNIAPSSSVASICIPSTVLSPPMPTEALATPGYFPTVVQPFVESNLLVPVGSIGGQVQVSQPAVSLAQQAPTTSSQQAALEQNEMPMWGPCCHWLFCWESTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGCAKFNCASEQVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_022411006.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022411006.1

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG 
CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT 
CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC 
CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA 
GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG 
GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT 
CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA 
CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT 
CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA 
GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA 
TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG 
CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG 
TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT 
CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA 
AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG 
CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT 
GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA 
CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT 
AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA 
GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT 
CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG 
AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT 
ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT 
AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA 
GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT 
AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT 
AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT 
GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT 
GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT 
ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT 
GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG 
GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC 
AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA 
GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA 
GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT 
TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG 
CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT 
CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG 
GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG 
CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT 
AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA 
GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG 
ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG 
TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC 
CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT 
AAAGCTCCAT CCCAGCAACC TCTGGAAGAT CTTGATGCTC AGTTGAGAAG AACACTTAGT 
CCAGAGACTA TTGCGGTGAC ATCTACAATT GGTCCTGTGT CCATGGTGGC TCCAACAGCA 
GTCACAGAAG CAGGAGCACA GCCTCAGAAG GATGTTTCTC AGATCACAGA AGGTCCTGTC 
CTAGCGACTA GTTCGGGAAC TGGTGTTTTT AAGATGGGAA GATTTCAGGT TTCAGTTGCA 
ATGGATGATG TTCAAAAGGA GGGTAAAAAT AAGTTAGAAG ATGGAAAGTC TGTTCATTTT 
GAATCCAGCA CTTCAGAGTC CTCAGTGCTA TCAAGTAGTA GTCCAGAAAG TACCCTGGTG 
AAGACAGAGC CTAATGGGAT AACCATCCCT GGCATCTCAT CAGATATGCC AGATAGTGCC 
CACAAAACTC CTGCCTCAGA AGCAAAGTCA GAAACTGGGC AGCCTACCAA GGTTGGACGT 
TTTCAGGTGA CAACTACAGC AAACAAAGTA GGTCGTTTCT CTGTGTCAAG AACTGAAGAC 
AAGCTCATTG AGGCAAAGAA AGAGGGACCA ATAGCGTCTC CTCCTTTTAT GGATTTGGAA 
CAAGGTGTTC TTCCAGCTGT GATACCAAAG AAAGAAAAGC CTGAACTGTC AGAGCCTTCA 
CATCTGAACG GACCATCTTC TGACCTGGAG GCTGCCTTCC TGAGCAGGGA TGTGGATGAT 
GGTTCAGGTA GTCCACACTC CCCCCATCAG CTGAGCTCAA AGAGCCTGCC TATCCAGAAT 
CTAAGTCAAA GCCTTAGTAA TTCATTCAAC TCCTCTTACA TGAGTAGTGA CAATGAGTCA 
GATATTGAAG ATGAAGATTT AAAATTAGAG CTGCGAAGAC TACGAGAAAA ACATCTTAAA 
GAGATTCAAG ACCTGCAGAG TCGCCAGAAG CATGAAATTG AATCTTTGTA TACCAAACTG 
GGAAAGGTTC CCCCTGCTGT CATTATCCCC CCAGCTGCCC CCCTTTCAGG GAGAAGAAGG 
CGACCCACTA AAAGCAAAGG CAGCAAGTCT AGTCGCAGCA GTTCCTTGGG GAATAAAAGT 
CCCCAGCTTT CAGGTAACCT GTCTGGTCAG AGTGCTGCTT CAGTTTTGCA CGCTCAGCAG 
ACCCTCCACC CTCCTGGCAA CATCCCTGAG ACTGGGCCAA ATCAGCTGTT ACAGCCCCTT 
AAGCCATCTC CCTCCAGTGA CAACCTCTAC TCAGCCTTTA CCAGTGATGG TGCCATTTCA 
GTCCCAAGCC TTTCTGCTCC AGGTCAAGGC TGTGCGAAGT TTAACTGTGC ATCTGAGCAG 
GTGACATTCA AACCTGGTGG CAGGAGGACC CGATTTCTGA GTACGCCCTG CTTGGCTCTT 
TGGAAGATGG TGAAAAAAGT CTGTCCTTGC AACCAGCTCT GTAGGACCAG CAGCACAAAC 
ACTGTCGGGG GAACAGTGAA CAGCCAAGCT GCCCCAGCTC AGCCTCCTGC CATGACATCC 
AGCAGGAAGG GCACATTCAC AGATGACCTA CACAAGTTGG TAGACAATTG GGCCCGAGAT 
GCCATGAATC TCTCAGGCAG AAGAGGAAGC AAAGGACACA TGAATTATGA GGGCCCTGGA 
ATGGCAAGAA AGTTCTCCGC ACCTGGTCAG CTGTGCATCT CCATGACCTC AAATCTGGGT 
GGTTCTGCCC CCATCTCTGC AGCATCAGCT ACCTCTCTAG GTCACTTCAC CAAGTCTATG 
TGCCCCCCAC AGCAGTACGG TTTTCCAGCT CCCCCCTTTG GCACTCAGTG GAGTGGGACG 
GGTGGCCCGG CACCACAGCC ACTTGGCCAG TTCCAGCCTG TGGGAACTGC CTCCTTACAG 
AATTTCAACA TCAGCAATTT GCAGAAATCC ATCAGCAACC CCCCAGGTTC CAACCTGCGG 
ACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_022411001.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022266709.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X4, mRNA.

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CloneID	OCe282743
Clone ID Related Accession (Same CDS sequence)	XM_022411001.1
Accession Version	XM_022411001.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase WNK1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG 
CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT 
CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC 
CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA 
GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG 
GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT 
CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA 
CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT 
CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA 
GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA 
TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG 
CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG 
TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT 
CTGGAGAGCA CTCAGGGAGT CTCTCAAGTT GCTCCTCCAG AGCCAGTTCC AGTGGCACAG 
GCACAGCCTA CCACTTTGGT CTCCTCTATA GACAGTGCAC ATTCAGATGT TGCTTCAGGA 
ATGAGTGATG GCAATGAGAA TGTCCCGTCC TCCAGTGGAA GGCATGAAGG GAGAGCTACA 
AAACGACATT ACCGAAAATC TGTAAGAAGT CGCTCTCGTC ATGAAAAGAC TTCGCGTCCA 
AAATTAAGAA TTTTGAATGT TTCAAATAAA GGAGACCGAG TAGTAGAATG TCAACTAGAG 
ACTCATAATC GGAAAATGGT TACATTCAAA TTTGATTTAG ATGGTGACAA TCCTGAGGAG 
ATAGCAACAA TTATGGTGAA CAATGACTTT ATTCTAGCAA TGGAGAGAGA GTCCTTTGTG 
GATCAAGTAC GAGAAATTAT TGAAAAGGCT GATGAAATGC TCAGTGAGGA TGTCAGTGTG 
GAACCAGAGG GTGACCAGGG ATTGGAGAGT CTGCAGGGAA AGGATGACTA TGGCTTTACA 
GGTTCTCAAA AATTGGAAGG AGAATTCAAA CAACCAGTTC CTGCGTCTTC CATGCCACAG 
CAAATAGGCA TTCCTACCAG TTCTTTAACT CAAGTTGTTC ATTCTGCTGG AAGACGGTTT 
ATAGTGAGTC CTGTGCCAGA AAGTCGATTA CGAGAATCAA AAGTTTTCAC TAGTGAAATA 
CCAGATACAG TTGCTGCCTC TACTCACAGC GCTGGAATGA ACTTGTCTCA CTCTGCTTCA 
TCCCTTAGCC TACAGCAAGC CTTTTCTGAA CTTAGACATG TGCAGATGAC AGAAGGGCCC 
AGCACAGCAC CTCCCAACTT CAGTCATACA GGACCAACAT TTCCAGTTGT TCCTCCTTCC 
ATGAGTAGCA TTGCTGGAGT CCCAACCACA GCAGTAGCCA CACCTTCAGT ATCAGTCCCT 
GCAACTAGCA GCCCTCTAAA TGACAGTTCC ACATCAGTAA TTCAATCTGA GATTCCAGTG 
CCCACTGAAA CAGGGGTTGG TGGAGTTCCC ACCTGCACAA GTGTAATGCC TTCAAGTGGT 
CTCCCTGTAC CACCCATATC TGAATCGCCA ATATTTTCCA CTATGGTTTC AAGCATCACA 
GTACCTACAG TTGTCTCAAT ATCAGCAACA TCCCAGCCAG TTCAAGCTCC CACATCTGGG 
AGCACTGTTT CTAGCATAGG AACTTTGCCT TCTATACCAG TTTCAACAGC TTTAGCCTCT 
GCAGTGGGCA GTGTTGCTAT CCCAGGTGCT AAGCCTCCAC CTGTACTATC ACAGCAGGCA 
GCAGGCAGTA CTACTGGGGC AGCCACATTA ACATCCGTTC CTGCCATCAC TCCGTTCCCA 
AGCATAGCTT CACAGCCATC TCTTCAGCTT AGCAGCAGCA CCTCTGCTCC AACTCTAGCT 
GAAACAGTGG TGGTTAGTGC ACACTCACTA GATAAAACAT CTCATAGCAG TACAACTGGA 
TTGGCTTTGT CCCTGTCTGC ATCATCTTCC TCCCCTGGAG CAGGATTATC TAGTTCTATT 
TCTCAGCCTG GTGGGGTGCA TCCTTTGGTT ACCCCATCAG CTATGGCTTC TATTCCTGCC 
CTTCCTCAAA CAGCAGGACC TACTTCTACA CCTTTATTGC CCCAAGTACT TAGTATCCCA 
CCCTTGGTAC AACCTGTTGC CAGTGTGCCT GCTGTGCAGC AGACACTAAT TCATAGTCAG 
CCTCAACCAG CTTTACTTCC CAACCAGCCC CACACTCACT GTCCTGAAAT AGATGCTGAC 
TTACAGCCCA AAGCTCCTGG AATTGATGAC ATAAAGACTC TAGAGGAAAA GCTGCGGTCT 
CTCTTTAGTG AACCGAGCTC ATCTGGAGCC CAACATACAT CGGTCTCACT AGAGACTTCA 
CTGGTAGTAG AGACCACTGT CACGCCAGGT ATCCCAACCA CTGCTATTGC ACCAAGCAAG 
CTCATGACAT CTACTACAAG TACATGCTTA CCACCAACCA GTTTGCAATT AGGAACATCT 
GGTTTGTCAG TTATACCAGT GGTCACGCCT GGGCAGGTTT CTACCCCAGT CAGCTATGTG 
TCAGCCCCAG GCAGCACTAC ATCAGGAGTG AAAGCTGGAA CTGCTCCATC AAAGCCACCT 
TTGACTAAAG CTCCAGTGCT GCCAGTGGGT ACTGAACTTC CAGCTGGTAC TCCACCCAGC 
GAGCAGCTGC CACCTTTTCC AGGACCTTCA CTAACTCAGT CCCAGCAACC TCTGGAAGAT 
CTTGATGCTC AGTTGAGAAG AACACTTAGT CCAGAGACTA TTGCGGTGAC ATCTACAATT 
GGTCCTGTGT CCATGGTGGC TCCAACAGCA GTCACAGAAG CAGGAGCACA GCCTCAGAAG 
GATGTTTCTC AGATCACAGA AGGTCCTGTC CTAGCGACTA GTTCGGGAAC TGGTGTTTTT 
AAGATGGGAA GATTTCAGGT TTCAGTTGCA ATGGATGATG TTCAAAAGGA GGGTAAAAAT 
AAGTTAGAAG ATGGAAAGTC TGTTCATTTT GAATCCAGCA CTTCAGAGTC CTCAGTGCTA 
TCAAGTAGTA GTCCAGAAAG TACCCTGGTG AAGACAGAGC CTAATGGGAT AACCATCCCT 
GGCATCTCAT CAGATATGCC AGATAGTGCC CACAAAACTC CTGCCTCAGA AGCAAAGTCA 
GAAACTGGGC AGCCTACCAA GGTTGGACGT TTTCAGGTGA CAACTACAGC AAACAAAGTA 
GGTCGTTTCT CTGTGTCAAG AACTGAAGAC AAGCTCATTG AGGCAAAGAA AGAGGGACCA 
ATAGCGTCTC CTCCTTTTAT GGATTTGGAA CAAGGTGTTC TTCCAGCTGT GATACCAAAG 
AAAGAAAAGC CTGAACTGTC AGAGCCTTCA CATCTGAACG GACCATCTTC TGACCTGGAG 
GCTGCCTTCC TGAGCAGGGA TGTGGATGAT GGTTCAGGTA GTCCACACTC CCCCCATCAG 
CTGAGCTCAA AGAGCCTGCC TATCCAGAAT CTAAGTCAAA GCCTTAGTAA TTCATTCAAC 
TCCTCTTACA TGAGTAGTGA CAATGAGTCA GATATTGAAG ATGAAGATTT AAAATTAGAG 
CTGCGAAGAC TACGAGAAAA ACATCTTAAA GAGATTCAAG ACCTGCAGAG TCGCCAGAAG 
CATGAAATTG AATCTTTGTA TACCAAACTG GGAAAGGTTC CCCCTGCTGT CATTATCCCC 
CCAGCTGCCC CCCTTTCAGG GAGAAGAAGG CGACCCACTA AAAGCAAAGG CAGCAAGTCT 
AGTCGCAGCA GTTCCTTGGG GAATAAAAGT CCCCAGCTTT CAGGTAACCT GTCTGGTCAG 
AGTGCTGCTT CAGTTTTGCA CGCTCAGCAG ACCCTCCACC CTCCTGGCAA CATCCCTGAG 
ACTGGGCCAA ATCAGCTGTT ACAGCCCCTT AAGCCATCTC CCTCCAGTGA CAACCTCTAC 
TCAGCCTTTA CCAGTGATGG TGCCATTTCA GTCCCAAGCC TTTCTGCTCC AGGTCAAGGC 
TGTGCGAAGT TTAACTGTGC ATCTGAGCAG GTGACATTCA AACCTGGTGG CAGGAGGACC 
CGATTTCTGA GTACGCCCTG CTTGGCTCTT TGGAAGATGG TGAAAAAAGT CTGTCCTTGC 
AACCAGCTCT GTAGGACCAG CAGCACAAAC ACTGTCGGGG GAACAGTGAA CAGCCAAGCT 
GCCCCAGCTC AGCCTCCTGC CATGACATCC AGCAGGAAGG GCACATTCAC AGATGACCTA 
CACAAGTTGG TAGACAATTG GGCCCGAGAT GCCATGAATC TCTCAGGCAG AAGAGGAAGC 
AAAGGACACA TGAATTATGA GGGCCCTGGA ATGGCAAGAA AGTTCTCCGC ACCTGGTCAG 
CTGTGCATCT CCATGACCTC AAATCTGGGT GGTTCTGCCC CCATCTCTGC AGCATCAGCT 
ACCTCTCTAG GTCACTTCAC CAAGTCTATG TGCCCCCCAC AGCAGTACGG TTTTCCAGCT 
CCCCCCTTTG GCACTCAGTG GAGTGGGACG GGTGGCCCGG CACCACAGCC ACTTGGCCAG 
TTCCAGCCTG TGGGAACTGC CTCCTTACAG AATTTCAACA TCAGCAATTT GCAGAAATCC 
ATCAGCAACC CCCCAGGTTC CAACCTGCGG ACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022266709.1
CDS	996..8294
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVGQSQGQPSSSSLTGIPSSLPIQHSQQQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQLPVSQPVPTIQGEPQIPVAAQPSVVPVHSGAHFLPVGQPLSTSLLPQYPVSQIPISTPHASAPQTGFSSLPITMAAGINQPLLTLASSATAAAIPGGPTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPSRLPPQYPGDSNIAPSSSVASICIPSTVLSPPMPTEALATPGYFPTVVQPFVESNLLVPVGSIGGQVQVSQPAVSLAQQAPTTSSQQAALESTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGCAKFNCASEQVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_022411001.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022411001.1

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG 
CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT 
CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC 
CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA 
GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG 
GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT 
CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA 
CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT 
CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA 
GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA 
TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG 
CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG 
TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT 
CTGGAGAGCA CTCAGGGAGT CTCTCAAGTT GCTCCTCCAG AGCCAGTTCC AGTGGCACAG 
GCACAGCCTA CCACTTTGGT CTCCTCTATA GACAGTGCAC ATTCAGATGT TGCTTCAGGA 
ATGAGTGATG GCAATGAGAA TGTCCCGTCC TCCAGTGGAA GGCATGAAGG GAGAGCTACA 
AAACGACATT ACCGAAAATC TGTAAGAAGT CGCTCTCGTC ATGAAAAGAC TTCGCGTCCA 
AAATTAAGAA TTTTGAATGT TTCAAATAAA GGAGACCGAG TAGTAGAATG TCAACTAGAG 
ACTCATAATC GGAAAATGGT TACATTCAAA TTTGATTTAG ATGGTGACAA TCCTGAGGAG 
ATAGCAACAA TTATGGTGAA CAATGACTTT ATTCTAGCAA TGGAGAGAGA GTCCTTTGTG 
GATCAAGTAC GAGAAATTAT TGAAAAGGCT GATGAAATGC TCAGTGAGGA TGTCAGTGTG 
GAACCAGAGG GTGACCAGGG ATTGGAGAGT CTGCAGGGAA AGGATGACTA TGGCTTTACA 
GGTTCTCAAA AATTGGAAGG AGAATTCAAA CAACCAGTTC CTGCGTCTTC CATGCCACAG 
CAAATAGGCA TTCCTACCAG TTCTTTAACT CAAGTTGTTC ATTCTGCTGG AAGACGGTTT 
ATAGTGAGTC CTGTGCCAGA AAGTCGATTA CGAGAATCAA AAGTTTTCAC TAGTGAAATA 
CCAGATACAG TTGCTGCCTC TACTCACAGC GCTGGAATGA ACTTGTCTCA CTCTGCTTCA 
TCCCTTAGCC TACAGCAAGC CTTTTCTGAA CTTAGACATG TGCAGATGAC AGAAGGGCCC 
AGCACAGCAC CTCCCAACTT CAGTCATACA GGACCAACAT TTCCAGTTGT TCCTCCTTCC 
ATGAGTAGCA TTGCTGGAGT CCCAACCACA GCAGTAGCCA CACCTTCAGT ATCAGTCCCT 
GCAACTAGCA GCCCTCTAAA TGACAGTTCC ACATCAGTAA TTCAATCTGA GATTCCAGTG 
CCCACTGAAA CAGGGGTTGG TGGAGTTCCC ACCTGCACAA GTGTAATGCC TTCAAGTGGT 
CTCCCTGTAC CACCCATATC TGAATCGCCA ATATTTTCCA CTATGGTTTC AAGCATCACA 
GTACCTACAG TTGTCTCAAT ATCAGCAACA TCCCAGCCAG TTCAAGCTCC CACATCTGGG 
AGCACTGTTT CTAGCATAGG AACTTTGCCT TCTATACCAG TTTCAACAGC TTTAGCCTCT 
GCAGTGGGCA GTGTTGCTAT CCCAGGTGCT AAGCCTCCAC CTGTACTATC ACAGCAGGCA 
GCAGGCAGTA CTACTGGGGC AGCCACATTA ACATCCGTTC CTGCCATCAC TCCGTTCCCA 
AGCATAGCTT CACAGCCATC TCTTCAGCTT AGCAGCAGCA CCTCTGCTCC AACTCTAGCT 
GAAACAGTGG TGGTTAGTGC ACACTCACTA GATAAAACAT CTCATAGCAG TACAACTGGA 
TTGGCTTTGT CCCTGTCTGC ATCATCTTCC TCCCCTGGAG CAGGATTATC TAGTTCTATT 
TCTCAGCCTG GTGGGGTGCA TCCTTTGGTT ACCCCATCAG CTATGGCTTC TATTCCTGCC 
CTTCCTCAAA CAGCAGGACC TACTTCTACA CCTTTATTGC CCCAAGTACT TAGTATCCCA 
CCCTTGGTAC AACCTGTTGC CAGTGTGCCT GCTGTGCAGC AGACACTAAT TCATAGTCAG 
CCTCAACCAG CTTTACTTCC CAACCAGCCC CACACTCACT GTCCTGAAAT AGATGCTGAC 
TTACAGCCCA AAGCTCCTGG AATTGATGAC ATAAAGACTC TAGAGGAAAA GCTGCGGTCT 
CTCTTTAGTG AACCGAGCTC ATCTGGAGCC CAACATACAT CGGTCTCACT AGAGACTTCA 
CTGGTAGTAG AGACCACTGT CACGCCAGGT ATCCCAACCA CTGCTATTGC ACCAAGCAAG 
CTCATGACAT CTACTACAAG TACATGCTTA CCACCAACCA GTTTGCAATT AGGAACATCT 
GGTTTGTCAG TTATACCAGT GGTCACGCCT GGGCAGGTTT CTACCCCAGT CAGCTATGTG 
TCAGCCCCAG GCAGCACTAC ATCAGGAGTG AAAGCTGGAA CTGCTCCATC AAAGCCACCT 
TTGACTAAAG CTCCAGTGCT GCCAGTGGGT ACTGAACTTC CAGCTGGTAC TCCACCCAGC 
GAGCAGCTGC CACCTTTTCC AGGACCTTCA CTAACTCAGT CCCAGCAACC TCTGGAAGAT 
CTTGATGCTC AGTTGAGAAG AACACTTAGT CCAGAGACTA TTGCGGTGAC ATCTACAATT 
GGTCCTGTGT CCATGGTGGC TCCAACAGCA GTCACAGAAG CAGGAGCACA GCCTCAGAAG 
GATGTTTCTC AGATCACAGA AGGTCCTGTC CTAGCGACTA GTTCGGGAAC TGGTGTTTTT 
AAGATGGGAA GATTTCAGGT TTCAGTTGCA ATGGATGATG TTCAAAAGGA GGGTAAAAAT 
AAGTTAGAAG ATGGAAAGTC TGTTCATTTT GAATCCAGCA CTTCAGAGTC CTCAGTGCTA 
TCAAGTAGTA GTCCAGAAAG TACCCTGGTG AAGACAGAGC CTAATGGGAT AACCATCCCT 
GGCATCTCAT CAGATATGCC AGATAGTGCC CACAAAACTC CTGCCTCAGA AGCAAAGTCA 
GAAACTGGGC AGCCTACCAA GGTTGGACGT TTTCAGGTGA CAACTACAGC AAACAAAGTA 
GGTCGTTTCT CTGTGTCAAG AACTGAAGAC AAGCTCATTG AGGCAAAGAA AGAGGGACCA 
ATAGCGTCTC CTCCTTTTAT GGATTTGGAA CAAGGTGTTC TTCCAGCTGT GATACCAAAG 
AAAGAAAAGC CTGAACTGTC AGAGCCTTCA CATCTGAACG GACCATCTTC TGACCTGGAG 
GCTGCCTTCC TGAGCAGGGA TGTGGATGAT GGTTCAGGTA GTCCACACTC CCCCCATCAG 
CTGAGCTCAA AGAGCCTGCC TATCCAGAAT CTAAGTCAAA GCCTTAGTAA TTCATTCAAC 
TCCTCTTACA TGAGTAGTGA CAATGAGTCA GATATTGAAG ATGAAGATTT AAAATTAGAG 
CTGCGAAGAC TACGAGAAAA ACATCTTAAA GAGATTCAAG ACCTGCAGAG TCGCCAGAAG 
CATGAAATTG AATCTTTGTA TACCAAACTG GGAAAGGTTC CCCCTGCTGT CATTATCCCC 
CCAGCTGCCC CCCTTTCAGG GAGAAGAAGG CGACCCACTA AAAGCAAAGG CAGCAAGTCT 
AGTCGCAGCA GTTCCTTGGG GAATAAAAGT CCCCAGCTTT CAGGTAACCT GTCTGGTCAG 
AGTGCTGCTT CAGTTTTGCA CGCTCAGCAG ACCCTCCACC CTCCTGGCAA CATCCCTGAG 
ACTGGGCCAA ATCAGCTGTT ACAGCCCCTT AAGCCATCTC CCTCCAGTGA CAACCTCTAC 
TCAGCCTTTA CCAGTGATGG TGCCATTTCA GTCCCAAGCC TTTCTGCTCC AGGTCAAGGC 
TGTGCGAAGT TTAACTGTGC ATCTGAGCAG GTGACATTCA AACCTGGTGG CAGGAGGACC 
CGATTTCTGA GTACGCCCTG CTTGGCTCTT TGGAAGATGG TGAAAAAAGT CTGTCCTTGC 
AACCAGCTCT GTAGGACCAG CAGCACAAAC ACTGTCGGGG GAACAGTGAA CAGCCAAGCT 
GCCCCAGCTC AGCCTCCTGC CATGACATCC AGCAGGAAGG GCACATTCAC AGATGACCTA 
CACAAGTTGG TAGACAATTG GGCCCGAGAT GCCATGAATC TCTCAGGCAG AAGAGGAAGC 
AAAGGACACA TGAATTATGA GGGCCCTGGA ATGGCAAGAA AGTTCTCCGC ACCTGGTCAG 
CTGTGCATCT CCATGACCTC AAATCTGGGT GGTTCTGCCC CCATCTCTGC AGCATCAGCT 
ACCTCTCTAG GTCACTTCAC CAAGTCTATG TGCCCCCCAC AGCAGTACGG TTTTCCAGCT 
CCCCCCTTTG GCACTCAGTG GAGTGGGACG GGTGGCCCGG CACCACAGCC ACTTGGCCAG 
TTCCAGCCTG TGGGAACTGC CTCCTTACAG AATTTCAACA TCAGCAATTT GCAGAAATCC 
ATCAGCAACC CCCCAGGTTC CAACCTGCGG ACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_022411002.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022266710.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X5, mRNA.

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CloneID	OCe282744
Clone ID Related Accession (Same CDS sequence)	XM_022411002.1
Accession Version	XM_022411002.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase WNK1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGGG AGTACAGCAG ACAGCCCCTC CACAACAGAC AGCGCAGTAT 
TCGCTTCCAC AGACATCAGC CTCCAGTGAG GCCACTATTG CACAACCAGT CAGTCAGTCT 
CAGGCTCCAC AGGTCTTGCC TCAAATATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA 
GTACCAACTA TCCAAGGCGA ACCTCAGATC CCAGTTGCGG CACAACCCTC AGTTGTTCCA 
GTCCACTCTG GTGCTCATTT CCTCCCTGTG GGACAGCCAC TCTCTACTTC CTTACTCCCT 
CAGTACCCGG TCTCTCAAAT TCCCATATCA ACTCCTCATG CGTCTGCGCC CCAGACAGGT 
TTTTCATCCC TTCCCATTAC AATGGCAGCT GGCATTAATC AGCCTCTGCT CACCTTGGCT 
TCATCTGCTA CAGCAGCTGC GATCCCAGGG GGACCAACTG TGGTTCCTAG TCAGCTTCCA 
ACCCTTCTGC AGCCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAACT CCTGCAACCA 
GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCTGAGGT TCCACTTCCC 
TCTGGAGATG TTCTGTACCA GGGCTTTCCA TCTCGACTGC CACCACAGTA CCCAGGAGAT 
TCAAATATTG CTCCTTCTTC CAGCGTGGCT TCTATTTGCA TCCCTTCTAC AGTCCTATCC 
CCTCCCATGC CCACAGAAGC ACTGGCTACA CCGGGTTACT TTCCTACAGT GGTGCAGCCT 
TTTGTGGAAT CTAACCTTTT GGTTCCTGTG GGCAGTATAG GAGGACAGGT TCAAGTGTCC 
CAGCCAGCAG TGAGTTTAGC ACAACAAGCC CCCACTACAT CCTCCCAGCA AGCAGCTCTG 
GAGAGCACTC AGGGAGTCTC TCAAGTTGCT CCTCCAGAGC CAGTTCCAGT GGCACAGGCA 
CAGCCTACCA CTTTGGTCTC CTCTATAGAC AGTGCACATT CAGATGTTGC TTCAGGAATG 
AGTGATGGCA ATGAGAATGT CCCGTCCTCC AGTGGAAGGC ATGAAGGGAG AGCTACAAAA 
CGACATTACC GAAAATCTGT AAGAAGTCGC TCTCGTCATG AAAAGACTTC GCGTCCAAAA 
TTAAGAATTT TGAATGTTTC AAATAAAGGA GACCGAGTAG TAGAATGTCA ACTAGAGACT 
CATAATCGGA AAATGGTTAC ATTCAAATTT GATTTAGATG GTGACAATCC TGAGGAGATA 
GCAACAATTA TGGTGAACAA TGACTTTATT CTAGCAATGG AGAGAGAGTC CTTTGTGGAT 
CAAGTACGAG AAATTATTGA AAAGGCTGAT GAAATGCTCA GTGAGGATGT CAGTGTGGAA 
CCAGAGGGTG ACCAGGGATT GGAGAGTCTG CAGGGAAAGG ATGACTATGG CTTTACAGGT 
TCTCAAAAAT TGGAAGGAGA ATTCAAACAA CCAGTTCCTG CGTCTTCCAT GCCACAGCAA 
ATAGGCATTC CTACCAGTTC TTTAACTCAA GTTGTTCATT CTGCTGGAAG ACGGTTTATA 
GTGAGTCCTG TGCCAGAAAG TCGATTACGA GAATCAAAAG TTTTCACTAG TGAAATACCA 
GATACAGTTG CTGCCTCTAC TCACAGCGCT GGAATGAACT TGTCTCACTC TGCTTCATCC 
CTTAGCCTAC AGCAAGCCTT TTCTGAACTT AGACATGTGC AGATGACAGA AGGGCCCAGC 
ACAGCACCTC CCAACTTCAG TCATACAGGA CCAACATTTC CAGTTGTTCC TCCTTCCATG 
AGTAGCATTG CTGGAGTCCC AACCACAGCA GTAGCCACAC CTTCAGTATC AGTCCCTGCA 
ACTAGCAGCC CTCTAAATGA CAGTTCCACA TCAGTAATTC AATCTGAGAT TCCAGTGCCC 
ACTGAAACAG GGGTTGGTGG AGTTCCCACC TGCACAAGTG TAATGCCTTC AAGTGGTCTC 
CCTGTACCAC CCATATCTGA ATCGCCAATA TTTTCCACTA TGGTTTCAAG CATCACAGTA 
CCTACAGTTG TCTCAATATC AGCAACATCC CAGCCAGTTC AAGCTCCCAC ATCTGGGAGC 
ACTGTTTCTA GCATAGGAAC TTTGCCTTCT ATACCAGTTT CAACAGCTTT AGCCTCTGCA 
GTGGGCAGTG TTGCTATCCC AGGTGCTAAG CCTCCACCTG TACTATCACA GCAGGCAGCA 
GGCAGTACTA CTGGGGCAGC CACATTAACA TCCGTTCCTG CCATCACTCC GTTCCCAAGC 
ATAGCTTCAC AGCCATCTCT TCAGCTTAGC AGCAGCACCT CTGCTCCAAC TCTAGCTGAA 
ACAGTGGTGG TTAGTGCACA CTCACTAGAT AAAACATCTC ATAGCAGTAC AACTGGATTG 
GCTTTGTCCC TGTCTGCATC ATCTTCCTCC CCTGGAGCAG GATTATCTAG TTCTATTTCT 
CAGCCTGGTG GGGTGCATCC TTTGGTTACC CCATCAGCTA TGGCTTCTAT TCCTGCCCTT 
CCTCAAACAG CAGGACCTAC TTCTACACCT TTATTGCCCC AAGTACTTAG TATCCCACCC 
TTGGTACAAC CTGTTGCCAG TGTGCCTGCT GTGCAGCAGA CACTAATTCA TAGTCAGCCT 
CAACCAGCTT TACTTCCCAA CCAGCCCCAC ACTCACTGTC CTGAAATAGA TGCTGACTTA 
CAGCCCAAAG CTCCTGGAAT TGATGACATA AAGACTCTAG AGGAAAAGCT GCGGTCTCTC 
TTTAGTGAAC CGAGCTCATC TGGAGCCCAA CATACATCGG TCTCACTAGA GACTTCACTG 
GTAGTAGAGA CCACTGTCAC GCCAGGTATC CCAACCACTG CTATTGCACC AAGCAAGCTC 
ATGACATCTA CTACAAGTAC ATGCTTACCA CCAACCAGTT TGCAATTAGG AACATCTGGT 
TTGTCAGTTA TACCAGTGGT CACGCCTGGG CAGGTTTCTA CCCCAGTCAG CTATGTGTCA 
GCCCCAGGCA GCACTACATC AGGAGTGAAA GCTGGAACTG CTCCATCAAA GCCACCTTTG 
ACTAAAGCTC CAGTGCTGCC AGTGGGTACT GAACTTCCAG CTGGTACTCC ACCCAGCGAG 
CAGCTGCCAC CTTTTCCAGG ACCTTCACTA ACTCAGTCCC AGCAACCTCT GGAAGATCTT 
GATGCTCAGT TGAGAAGAAC ACTTAGTCCA GAGACTATTG CGGTGACATC TACAATTGGT 
CCTGTGTCCA TGGTGGCTCC AACAGCAGTC ACAGAAGCAG GAGCACAGCC TCAGAAGGAT 
GTTTCTCAGA TCACAGAAGG TCCTGTCCTA GCGACTAGTT CGGGAACTGG TGTTTTTAAG 
ATGGGAAGAT TTCAGGTTTC AGTTGCAATG GATGATGTTC AAAAGGAGGG TAAAAATAAG 
TTAGAAGATG GAAAGTCTGT TCATTTTGAA TCCAGCACTT CAGAGTCCTC AGTGCTATCA 
AGTAGTAGTC CAGAAAGTAC CCTGGTGAAG ACAGAGCCTA ATGGGATAAC CATCCCTGGC 
ATCTCATCAG ATATGCCAGA TAGTGCCCAC AAAACTCCTG CCTCAGAAGC AAAGTCAGAA 
ACTGGGCAGC CTACCAAGGT TGGACGTTTT CAGGTGACAA CTACAGCAAA CAAAGTAGGT 
CGTTTCTCTG TGTCAAGAAC TGAAGACAAG CTCATTGAGG CAAAGAAAGA GGGACCAATA 
GCGTCTCCTC CTTTTATGGA TTTGGAACAA GGTGTTCTTC CAGCTGTGAT ACCAAAGAAA 
GAAAAGCCTG AACTGTCAGA GCCTTCACAT CTGAACGGAC CATCTTCTGA CCTGGAGGCT 
GCCTTCCTGA GCAGGGATGT GGATGATGGT TCAGGTAGTC CACACTCCCC CCATCAGCTG 
AGCTCAAAGA GCCTGCCTAT CCAGAATCTA AGTCAAAGCC TTAGTAATTC ATTCAACTCC 
TCTTACATGA GTAGTGACAA TGAGTCAGAT ATTGAAGATG AAGATTTAAA ATTAGAGCTG 
CGAAGACTAC GAGAAAAACA TCTTAAAGAG ATTCAAGACC TGCAGAGTCG CCAGAAGCAT 
GAAATTGAAT CTTTGTATAC CAAACTGGGA AAGGTTCCCC CTGCTGTCAT TATCCCCCCA 
GCTGCCCCCC TTTCAGGGAG AAGAAGGCGA CCCACTAAAA GCAAAGGCAG CAAGTCTAGT 
CGCAGCAGTT CCTTGGGGAA TAAAAGTCCC CAGCTTTCAG GTAACCTGTC TGGTCAGAGT 
GCTGCTTCAG TTTTGCACGC TCAGCAGACC CTCCACCCTC CTGGCAACAT CCCTGAGACT 
GGGCCAAATC AGCTGTTACA GCCCCTTAAG CCATCTCCCT CCAGTGACAA CCTCTACTCA 
GCCTTTACCA GTGATGGTGC CATTTCAGTC CCAAGCCTTT CTGCTCCAGG TCAAGGCTGT 
GCGAAGTTTA ACTGTGCATC TGAGCAGGTG ACATTCAAAC CTGGTGGCAG GAGGACCCGA 
TTTCTGAGTA CGCCCTGCTT GGCTCTTTGG AAGATGGTGA AAAAAGTCTG TCCTTGCAAC 
CAGCTCTGTA GGACCAGCAG CACAAACACT GTCGGGGGAA CAGTGAACAG CCAAGCTGCC 
CCAGCTCAGC CTCCTGCCAT GACATCCAGC AGGAAGGGCA CATTCACAGA TGACCTACAC 
AAGTTGGTAG ACAATTGGGC CCGAGATGCC ATGAATCTCT CAGGCAGAAG AGGAAGCAAA 
GGACACATGA ATTATGAGGG CCCTGGAATG GCAAGAAAGT TCTCCGCACC TGGTCAGCTG 
TGCATCTCCA TGACCTCAAA TCTGGGTGGT TCTGCCCCCA TCTCTGCAGC ATCAGCTACC 
TCTCTAGGTC ACTTCACCAA GTCTATGTGC CCCCCACAGC AGTACGGTTT TCCAGCTCCC 
CCCTTTGGCA CTCAGTGGAG TGGGACGGGT GGCCCGGCAC CACAGCCACT TGGCCAGTTC 
CAGCCTGTGG GAACTGCCTC CTTACAGAAT TTCAACATCA GCAATTTGCA GAAATCCATC 
AGCAACCCCC CAGGTTCCAA CCTGCGGACC ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022266710.1
CDS	996..8291
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVGQSQGQPSSSSLTGIPSSLPIQHSQQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQLPVSQPVPTIQGEPQIPVAAQPSVVPVHSGAHFLPVGQPLSTSLLPQYPVSQIPISTPHASAPQTGFSSLPITMAAGINQPLLTLASSATAAAIPGGPTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPSRLPPQYPGDSNIAPSSSVASICIPSTVLSPPMPTEALATPGYFPTVVQPFVESNLLVPVGSIGGQVQVSQPAVSLAQQAPTTSSQQAALESTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGCAKFNCASEQVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_022411002.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022411002.1

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGGG AGTACAGCAG ACAGCCCCTC CACAACAGAC AGCGCAGTAT 
TCGCTTCCAC AGACATCAGC CTCCAGTGAG GCCACTATTG CACAACCAGT CAGTCAGTCT 
CAGGCTCCAC AGGTCTTGCC TCAAATATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA 
GTACCAACTA TCCAAGGCGA ACCTCAGATC CCAGTTGCGG CACAACCCTC AGTTGTTCCA 
GTCCACTCTG GTGCTCATTT CCTCCCTGTG GGACAGCCAC TCTCTACTTC CTTACTCCCT 
CAGTACCCGG TCTCTCAAAT TCCCATATCA ACTCCTCATG CGTCTGCGCC CCAGACAGGT 
TTTTCATCCC TTCCCATTAC AATGGCAGCT GGCATTAATC AGCCTCTGCT CACCTTGGCT 
TCATCTGCTA CAGCAGCTGC GATCCCAGGG GGACCAACTG TGGTTCCTAG TCAGCTTCCA 
ACCCTTCTGC AGCCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAACT CCTGCAACCA 
GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCTGAGGT TCCACTTCCC 
TCTGGAGATG TTCTGTACCA GGGCTTTCCA TCTCGACTGC CACCACAGTA CCCAGGAGAT 
TCAAATATTG CTCCTTCTTC CAGCGTGGCT TCTATTTGCA TCCCTTCTAC AGTCCTATCC 
CCTCCCATGC CCACAGAAGC ACTGGCTACA CCGGGTTACT TTCCTACAGT GGTGCAGCCT 
TTTGTGGAAT CTAACCTTTT GGTTCCTGTG GGCAGTATAG GAGGACAGGT TCAAGTGTCC 
CAGCCAGCAG TGAGTTTAGC ACAACAAGCC CCCACTACAT CCTCCCAGCA AGCAGCTCTG 
GAGAGCACTC AGGGAGTCTC TCAAGTTGCT CCTCCAGAGC CAGTTCCAGT GGCACAGGCA 
CAGCCTACCA CTTTGGTCTC CTCTATAGAC AGTGCACATT CAGATGTTGC TTCAGGAATG 
AGTGATGGCA ATGAGAATGT CCCGTCCTCC AGTGGAAGGC ATGAAGGGAG AGCTACAAAA 
CGACATTACC GAAAATCTGT AAGAAGTCGC TCTCGTCATG AAAAGACTTC GCGTCCAAAA 
TTAAGAATTT TGAATGTTTC AAATAAAGGA GACCGAGTAG TAGAATGTCA ACTAGAGACT 
CATAATCGGA AAATGGTTAC ATTCAAATTT GATTTAGATG GTGACAATCC TGAGGAGATA 
GCAACAATTA TGGTGAACAA TGACTTTATT CTAGCAATGG AGAGAGAGTC CTTTGTGGAT 
CAAGTACGAG AAATTATTGA AAAGGCTGAT GAAATGCTCA GTGAGGATGT CAGTGTGGAA 
CCAGAGGGTG ACCAGGGATT GGAGAGTCTG CAGGGAAAGG ATGACTATGG CTTTACAGGT 
TCTCAAAAAT TGGAAGGAGA ATTCAAACAA CCAGTTCCTG CGTCTTCCAT GCCACAGCAA 
ATAGGCATTC CTACCAGTTC TTTAACTCAA GTTGTTCATT CTGCTGGAAG ACGGTTTATA 
GTGAGTCCTG TGCCAGAAAG TCGATTACGA GAATCAAAAG TTTTCACTAG TGAAATACCA 
GATACAGTTG CTGCCTCTAC TCACAGCGCT GGAATGAACT TGTCTCACTC TGCTTCATCC 
CTTAGCCTAC AGCAAGCCTT TTCTGAACTT AGACATGTGC AGATGACAGA AGGGCCCAGC 
ACAGCACCTC CCAACTTCAG TCATACAGGA CCAACATTTC CAGTTGTTCC TCCTTCCATG 
AGTAGCATTG CTGGAGTCCC AACCACAGCA GTAGCCACAC CTTCAGTATC AGTCCCTGCA 
ACTAGCAGCC CTCTAAATGA CAGTTCCACA TCAGTAATTC AATCTGAGAT TCCAGTGCCC 
ACTGAAACAG GGGTTGGTGG AGTTCCCACC TGCACAAGTG TAATGCCTTC AAGTGGTCTC 
CCTGTACCAC CCATATCTGA ATCGCCAATA TTTTCCACTA TGGTTTCAAG CATCACAGTA 
CCTACAGTTG TCTCAATATC AGCAACATCC CAGCCAGTTC AAGCTCCCAC ATCTGGGAGC 
ACTGTTTCTA GCATAGGAAC TTTGCCTTCT ATACCAGTTT CAACAGCTTT AGCCTCTGCA 
GTGGGCAGTG TTGCTATCCC AGGTGCTAAG CCTCCACCTG TACTATCACA GCAGGCAGCA 
GGCAGTACTA CTGGGGCAGC CACATTAACA TCCGTTCCTG CCATCACTCC GTTCCCAAGC 
ATAGCTTCAC AGCCATCTCT TCAGCTTAGC AGCAGCACCT CTGCTCCAAC TCTAGCTGAA 
ACAGTGGTGG TTAGTGCACA CTCACTAGAT AAAACATCTC ATAGCAGTAC AACTGGATTG 
GCTTTGTCCC TGTCTGCATC ATCTTCCTCC CCTGGAGCAG GATTATCTAG TTCTATTTCT 
CAGCCTGGTG GGGTGCATCC TTTGGTTACC CCATCAGCTA TGGCTTCTAT TCCTGCCCTT 
CCTCAAACAG CAGGACCTAC TTCTACACCT TTATTGCCCC AAGTACTTAG TATCCCACCC 
TTGGTACAAC CTGTTGCCAG TGTGCCTGCT GTGCAGCAGA CACTAATTCA TAGTCAGCCT 
CAACCAGCTT TACTTCCCAA CCAGCCCCAC ACTCACTGTC CTGAAATAGA TGCTGACTTA 
CAGCCCAAAG CTCCTGGAAT TGATGACATA AAGACTCTAG AGGAAAAGCT GCGGTCTCTC 
TTTAGTGAAC CGAGCTCATC TGGAGCCCAA CATACATCGG TCTCACTAGA GACTTCACTG 
GTAGTAGAGA CCACTGTCAC GCCAGGTATC CCAACCACTG CTATTGCACC AAGCAAGCTC 
ATGACATCTA CTACAAGTAC ATGCTTACCA CCAACCAGTT TGCAATTAGG AACATCTGGT 
TTGTCAGTTA TACCAGTGGT CACGCCTGGG CAGGTTTCTA CCCCAGTCAG CTATGTGTCA 
GCCCCAGGCA GCACTACATC AGGAGTGAAA GCTGGAACTG CTCCATCAAA GCCACCTTTG 
ACTAAAGCTC CAGTGCTGCC AGTGGGTACT GAACTTCCAG CTGGTACTCC ACCCAGCGAG 
CAGCTGCCAC CTTTTCCAGG ACCTTCACTA ACTCAGTCCC AGCAACCTCT GGAAGATCTT 
GATGCTCAGT TGAGAAGAAC ACTTAGTCCA GAGACTATTG CGGTGACATC TACAATTGGT 
CCTGTGTCCA TGGTGGCTCC AACAGCAGTC ACAGAAGCAG GAGCACAGCC TCAGAAGGAT 
GTTTCTCAGA TCACAGAAGG TCCTGTCCTA GCGACTAGTT CGGGAACTGG TGTTTTTAAG 
ATGGGAAGAT TTCAGGTTTC AGTTGCAATG GATGATGTTC AAAAGGAGGG TAAAAATAAG 
TTAGAAGATG GAAAGTCTGT TCATTTTGAA TCCAGCACTT CAGAGTCCTC AGTGCTATCA 
AGTAGTAGTC CAGAAAGTAC CCTGGTGAAG ACAGAGCCTA ATGGGATAAC CATCCCTGGC 
ATCTCATCAG ATATGCCAGA TAGTGCCCAC AAAACTCCTG CCTCAGAAGC AAAGTCAGAA 
ACTGGGCAGC CTACCAAGGT TGGACGTTTT CAGGTGACAA CTACAGCAAA CAAAGTAGGT 
CGTTTCTCTG TGTCAAGAAC TGAAGACAAG CTCATTGAGG CAAAGAAAGA GGGACCAATA 
GCGTCTCCTC CTTTTATGGA TTTGGAACAA GGTGTTCTTC CAGCTGTGAT ACCAAAGAAA 
GAAAAGCCTG AACTGTCAGA GCCTTCACAT CTGAACGGAC CATCTTCTGA CCTGGAGGCT 
GCCTTCCTGA GCAGGGATGT GGATGATGGT TCAGGTAGTC CACACTCCCC CCATCAGCTG 
AGCTCAAAGA GCCTGCCTAT CCAGAATCTA AGTCAAAGCC TTAGTAATTC ATTCAACTCC 
TCTTACATGA GTAGTGACAA TGAGTCAGAT ATTGAAGATG AAGATTTAAA ATTAGAGCTG 
CGAAGACTAC GAGAAAAACA TCTTAAAGAG ATTCAAGACC TGCAGAGTCG CCAGAAGCAT 
GAAATTGAAT CTTTGTATAC CAAACTGGGA AAGGTTCCCC CTGCTGTCAT TATCCCCCCA 
GCTGCCCCCC TTTCAGGGAG AAGAAGGCGA CCCACTAAAA GCAAAGGCAG CAAGTCTAGT 
CGCAGCAGTT CCTTGGGGAA TAAAAGTCCC CAGCTTTCAG GTAACCTGTC TGGTCAGAGT 
GCTGCTTCAG TTTTGCACGC TCAGCAGACC CTCCACCCTC CTGGCAACAT CCCTGAGACT 
GGGCCAAATC AGCTGTTACA GCCCCTTAAG CCATCTCCCT CCAGTGACAA CCTCTACTCA 
GCCTTTACCA GTGATGGTGC CATTTCAGTC CCAAGCCTTT CTGCTCCAGG TCAAGGCTGT 
GCGAAGTTTA ACTGTGCATC TGAGCAGGTG ACATTCAAAC CTGGTGGCAG GAGGACCCGA 
TTTCTGAGTA CGCCCTGCTT GGCTCTTTGG AAGATGGTGA AAAAAGTCTG TCCTTGCAAC 
CAGCTCTGTA GGACCAGCAG CACAAACACT GTCGGGGGAA CAGTGAACAG CCAAGCTGCC 
CCAGCTCAGC CTCCTGCCAT GACATCCAGC AGGAAGGGCA CATTCACAGA TGACCTACAC 
AAGTTGGTAG ACAATTGGGC CCGAGATGCC ATGAATCTCT CAGGCAGAAG AGGAAGCAAA 
GGACACATGA ATTATGAGGG CCCTGGAATG GCAAGAAAGT TCTCCGCACC TGGTCAGCTG 
TGCATCTCCA TGACCTCAAA TCTGGGTGGT TCTGCCCCCA TCTCTGCAGC ATCAGCTACC 
TCTCTAGGTC ACTTCACCAA GTCTATGTGC CCCCCACAGC AGTACGGTTT TCCAGCTCCC 
CCCTTTGGCA CTCAGTGGAG TGGGACGGGT GGCCCGGCAC CACAGCCACT TGGCCAGTTC 
CAGCCTGTGG GAACTGCCTC CTTACAGAAT TTCAACATCA GCAATTTGCA GAAATCCATC 
AGCAACCCCC CAGGTTCCAA CCTGCGGACC ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_022411007.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022266715.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X12, mRNA.

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CloneID	OCe282745
Clone ID Related Accession (Same CDS sequence)	XM_022411007.1
Accession Version	XM_022411007.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase WNK1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG 
CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT 
CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC 
CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA 
GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG 
GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT 
CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA 
CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT 
CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA 
GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA 
TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG 
CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG 
TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT 
CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA 
AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG 
CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT 
GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA 
CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT 
AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA 
GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT 
CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG 
AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT 
ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT 
AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA 
GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT 
AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT 
AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT 
GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT 
GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT 
ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT 
GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG 
GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC 
AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA 
GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA 
GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT 
TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG 
CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT 
CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG 
GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG 
CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT 
AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA 
GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG 
ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG 
TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC 
CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT 
AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG 
CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT 
GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT 
GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT 
TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG 
GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA 
GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT 
AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC 
TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT 
GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT 
TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG 
TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA 
AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC 
TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC 
TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT 
TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA 
AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA 
ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT 
GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC 
AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT 
GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG 
CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC 
TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG 
AAGTTTAACT GTGCATCTGA GCAGGTGACA TTCAAACCTG GTGGCAGGAG GACCCGATTT 
CTGAGTACGC CCTGCTTGGC TCTTTGGAAG ATGGTGAAAA AAGTCTGTCC TTGCAACCAG 
CTCTGTATGT GCCCTCCAGC AGAGCCGAAA TCAGGCAGGA CACAGACCCC TTCTTCCTTT 
CTCATCAAAC CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022266715.1
CDS	998..7912
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVGQSQGQPSSSSLTGIPSSLPIQHSQQQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQLPVSQPVPTIQGEPQIPVAAQPSVVPVHSGAHFLPVGQPLSTSLLPQYPVSQIPISTPHASAPQTGFSSLPITMAAGINQPLLTLASSATAAAIPGGPTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPSRLPPQYPGDSNIAPSSSVASICIPSTVLSPPMPTEALATPGYFPTVVQPFVESNLLVPVGSIGGQVQVSQPAVSLAQQAPTTSSQQAALEQNEMPMWGPCCHWLFCWESTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGCAKFNCASEQVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCMCPPAEPKSGRTQTPSSFLIKP

Target ORF information:

RefSeq Version	XM_022411007.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022411007.1

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG 
CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT 
CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC 
CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA 
GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG 
GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT 
CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA 
CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT 
CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA 
GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA 
TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG 
CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG 
TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT 
CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA 
AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG 
CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT 
GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA 
CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT 
AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA 
GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT 
CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG 
AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT 
ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT 
AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA 
GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT 
AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT 
AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT 
GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT 
GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT 
ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT 
GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG 
GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC 
AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA 
GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA 
GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT 
TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG 
CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT 
CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG 
GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG 
CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT 
AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA 
GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG 
ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG 
TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC 
CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT 
AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG 
CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT 
GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT 
GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT 
TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG 
GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA 
GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT 
AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC 
TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT 
GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT 
TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG 
TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA 
AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC 
TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC 
TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT 
TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA 
AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA 
ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT 
GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC 
AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT 
GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG 
CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC 
TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG 
AAGTTTAACT GTGCATCTGA GCAGGTGACA TTCAAACCTG GTGGCAGGAG GACCCGATTT 
CTGAGTACGC CCTGCTTGGC TCTTTGGAAG ATGGTGAAAA AAGTCTGTCC TTGCAACCAG 
CTCTGTATGT GCCCTCCAGC AGAGCCGAAA TCAGGCAGGA CACAGACCCC TTCTTCCTTT 
CTCATCAAAC CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_022411008.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022266716.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X13, mRNA.

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CloneID	OCe282746
Clone ID Related Accession (Same CDS sequence)	XM_022411008.1
Accession Version	XM_022411008.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase WNK1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGGGCTT TCCATCTCGA 
CTGCCACCAC AGTACCCAGG AGATTCAAAT ATTGCTCCTT CTTCCAGCGT GGCTTCTATT 
TGCATCCCTT CTACAGTCCT ATCCCCTCCC ATGCCCACAG AAGCACTGGC TACACCGGGT 
TACTTTCCTA CAGTGGTGCA GCCTTTTGTG GAATCTAACC TTTTGGTTCC TGTGGGCAGT 
ATAGGAGGAC AGGTTCAAGT GTCCCAGCCA GCAGTGAGTT TAGCACAACA AGCCCCCACT 
ACATCCTCCC AGCAAGCAGC TCTGGAGCAG AATGAAATGC CAATGTGGGG GCCTTGTTGC 
CACTGGCTGT TCTGTTGGGA AAGCACTCAG GGAGTCTCTC AAGTTGCTCC TCCAGAGCCA 
GTTCCAGTGG CACAGGCACA GCCTACCACT TTGGTCTCCT CTATAGACAG TGCACATTCA 
GATGTTGCTT CAGGAATGAG TGATGGCAAT GAGAATGTCC CGTCCTCCAG TGGAAGGCAT 
GAAGGGAGAG CTACAAAACG ACATTACCGA AAATCTGTAA GAAGTCGCTC TCGTCATGAA 
AAGACTTCGC GTCCAAAATT AAGAATTTTG AATGTTTCAA ATAAAGGAGA CCGAGTAGTA 
GAATGTCAAC TAGAGACTCA TAATCGGAAA ATGGTTACAT TCAAATTTGA TTTAGATGGT 
GACAATCCTG AGGAGATAGC AACAATTATG GTGAACAATG ACTTTATTCT AGCAATGGAG 
AGAGAGTCCT TTGTGGATCA AGTACGAGAA ATTATTGAAA AGGCTGATGA AATGCTCAGT 
GAGGATGTCA GTGTGGAACC AGAGGGTGAC CAGGGATTGG AGAGTCTGCA GGGAAAGGAT 
GACTATGGCT TTACAGGTTC TCAAAAATTG GAAGGAGAAT TCAAACAACC AGTTCCTGCG 
TCTTCCATGC CACAGCAAAT AGGCATTCCT ACCAGTTCTT TAACTCAAGT TGTTCATTCT 
GCTGGAAGAC GGTTTATAGT GAGTCCTGTG CCAGAAAGTC GATTACGAGA ATCAAAAGTT 
TTCACTAGTG AAATACCAGA TACAGTTGCT GCCTCTACTC ACAGCGCTGG AATGAACTTG 
TCTCACTCTG CTTCATCCCT TAGCCTACAG CAAGCCTTTT CTGAACTTAG ACATGTGCAG 
ATGACAGAAG GGCCCAGCAC AGCACCTCCC AACTTCAGTC ATACAGGACC AACATTTCCA 
GTTGTTCCTC CTTCCATGAG TAGCATTGCT GGAGTCCCAA CCACAGCAGT AGCCACACCT 
TCAGTATCAG TCCCTGCAAC TAGCAGCCCT CTAAATGACA GTTCCACATC AGTAATTCAA 
TCTGAGATTC CAGTGCCCAC TGAAACAGGG GTTGGTGGAG TTCCCACCTG CACAAGTGTA 
ATGCCTTCAA GTGGTCTCCC TGTACCACCC ATATCTGAAT CGCCAATATT TTCCACTATG 
GTTTCAAGCA TCACAGTACC TACAGTTGTC TCAATATCAG CAACATCCCA GCCAGTTCAA 
GCTCCCACAT CTGGGAGCAC TGTTTCTAGC ATAGGAACTT TGCCTTCTAT ACCAGTTTCA 
ACAGCTTTAG CCTCTGCAGT GGGCAGTGTT GCTATCCCAG GTGCTAAGCC TCCACCTGTA 
CTATCACAGC AGGCAGCAGG CAGTACTACT GGGGCAGCCA CATTAACATC CGTTCCTGCC 
ATCACTCCGT TCCCAAGCAT AGCTTCACAG CCATCTCTTC AGCTTAGCAG CAGCACCTCT 
GCTCCAACTC TAGCTGAAAC AGTGGTGGTT AGTGCACACT CACTAGATAA AACATCTCAT 
AGCAGTACAA CTGGATTGGC TTTGTCCCTG TCTGCATCAT CTTCCTCCCC TGGAGCAGGA 
TTATCTAGTT CTATTTCTCA GCCTGGTGGG GTGCATCCTT TGGTTACCCC ATCAGCTATG 
GCTTCTATTC CTGCCCTTCC TCAAACAGCA GGACCTACTT CTACACCTTT ATTGCCCCAA 
GTACTTAGTA TCCCACCCTT GGTACAACCT GTTGCCAGTG TGCCTGCTGT GCAGCAGACA 
CTAATTCATA GTCAGCCTCA ACCAGCTTTA CTTCCCAACC AGCCCCACAC TCACTGTCCT 
GAAATAGATG CTGACTTACA GCCCAAAGCT CCTGGAATTG ATGACATAAA GACTCTAGAG 
GAAAAGCTGC GGTCTCTCTT TAGTGAACCG AGCTCATCTG GAGCCCAACA TACATCGGTC 
TCACTAGAGA CTTCACTGGT AGTAGAGACC ACTGTCACGC CAGGTATCCC AACCACTGCT 
ATTGCACCAA GCAAGCTCAT GACATCTACT ACAAGTACAT GCTTACCACC AACCAGTTTG 
CAATTAGGAA CATCTGGTTT GTCAGTTATA CCAGTGGTCA CGCCTGGGCA GGTTTCTACC 
CCAGTCAGCT ATGTGTCAGC CCCAGGCAGC ACTACATCAG GAGTGAAAGC TGGAACTGCT 
CCATCAAAGC CACCTTTGAC TAAAGCTCCA GTGCTGCCAG TGGGTACTGA ACTTCCAGCT 
GGTACTCCAC CCAGCGAGCA GCTGCCACCT TTTCCAGGAC CTTCACTAAC TCAGTCCCAG 
CAACCTCTGG AAGATCTTGA TGCTCAGTTG AGAAGAACAC TTAGTCCAGA GACTATTGCG 
GTGACATCTA CAATTGGTCC TGTGTCCATG GTGGCTCCAA CAGCAGTCAC AGAAGCAGGA 
GCACAGCCTC AGAAGGATGT TTCTCAGATC ACAGAAGGTC CTGTCCTAGC GACTAGTTCG 
GGAACTGGTG TTTTTAAGAT GGGAAGATTT CAGGTTTCAG TTGCAATGGA TGATGTTCAA 
AAGGAGGGTA AAAATAAGTT AGAAGATGGA AAGTCTGTTC ATTTTGAATC CAGCACTTCA 
GAGTCCTCAG TGCTATCAAG TAGTAGTCCA GAAAGTACCC TGGTGAAGAC AGAGCCTAAT 
GGGATAACCA TCCCTGGCAT CTCATCAGAT ATGCCAGATA GTGCCCACAA AACTCCTGCC 
TCAGAAGCAA AGTCAGAAAC TGGGCAGCCT ACCAAGGTTG GACGTTTTCA GGTGACAACT 
ACAGCAAACA AAGTAGGTCG TTTCTCTGTG TCAAGAACTG AAGACAAGCT CATTGAGGCA 
AAGAAAGAGG GACCAATAGC GTCTCCTCCT TTTATGGATT TGGAACAAGG TGTTCTTCCA 
GCTGTGATAC CAAAGAAAGA AAAGCCTGAA CTGTCAGAGC CTTCACATCT GAACGGACCA 
TCTTCTGACC TGGAGGCTGC CTTCCTGAGC AGGGATGTGG ATGATGGTTC AGGTAGTCCA 
CACTCCCCCC ATCAGCTGAG CTCAAAGAGC CTGCCTATCC AGAATCTAAG TCAAAGCCTT 
AGTAATTCAT TCAACTCCTC TTACATGAGT AGTGACAATG AGTCAGATAT TGAAGATGAA 
GATTTAAAAT TAGAGCTGCG AAGACTACGA GAAAAACATC TTAAAGAGAT TCAAGACCTG 
CAGAGTCGCC AGAAGCATGA AATTGAATCT TTGTATACCA AACTGGGAAA GGTTCCCCCT 
GCTGTCATTA TCCCCCCAGC TGCCCCCCTT TCAGGGAGAA GAAGGCGACC CACTAAAAGC 
AAAGGCAGCA AGTCTAGTCG CAGCAGTTCC TTGGGGAATA AAAGTCCCCA GCTTTCAGGT 
AACCTGTCTG GTCAGAGTGC TGCTTCAGTT TTGCACGCTC AGCAGACCCT CCACCCTCCT 
GGCAACATCC CTGAGACTGG GCCAAATCAG CTGTTACAGC CCCTTAAGCC ATCTCCCTCC 
AGTGACAACC TCTACTCAGC CTTTACCAGT GATGGTGCCA TTTCAGTCCC AAGCCTTTCT 
GCTCCAGGTC AAGGCTGTGC GAAGTTTAAC TGTGCATCTG AGCAGGTGAC ATTCAAACCT 
GGTGGCAGGA GGACCCGATT TCTGAGTACG CCCTGCTTGG CTCTTTGGAA GATGGTGAAA 
AAAGTCTGTC CTTGCAACCA GCTCTGTAGG ACCAGCAGCA CAAACACTGT CGGGGGAACA 
GTGAACAGCC AAGCTGCCCC AGCTCAGCCT CCTGCCATGA CATCCAGCAG GAAGGGCACA 
TTCACAGATG ACCTACACAA GTTGGTAGAC AATTGGGCCC GAGATGCCAT GAATCTCTCA 
GGCAGAAGAG GAAGCAAAGG ACACATGAAT TATGAGGGCC CTGGAATGGC AAGAAAGTTC 
TCCGCACCTG GTCAGCTGTG CATCTCCATG ACCTCAAATC TGGGTGGTTC TGCCCCCATC 
TCTGCAGCAT CAGCTACCTC TCTAGGTCAC TTCACCAAGT CTATGTGCCC CCCACAGCAG 
TACGGTTTTC CAGCTCCCCC CTTTGGCACT CAGTGGAGTG GGACGGGTGG CCCGGCACCA 
CAGCCACTTG GCCAGTTCCA GCCTGTGGGA ACTGCCTCCT TACAGAATTT CAACATCAGC 
AATTTGCAGA AATCCATCAG CAACCCCCCA GGTTCCAACC TGCGGACCAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022266716.1
CDS	996..7889
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVGQSQGQPSSSSLTGIPSSLPIQHSQQQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQGFPSRLPPQYPGDSNIAPSSSVASICIPSTVLSPPMPTEALATPGYFPTVVQPFVESNLLVPVGSIGGQVQVSQPAVSLAQQAPTTSSQQAALEQNEMPMWGPCCHWLFCWESTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGCAKFNCASEQVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_022411008.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022411008.1

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGGGCTT TCCATCTCGA 
CTGCCACCAC AGTACCCAGG AGATTCAAAT ATTGCTCCTT CTTCCAGCGT GGCTTCTATT 
TGCATCCCTT CTACAGTCCT ATCCCCTCCC ATGCCCACAG AAGCACTGGC TACACCGGGT 
TACTTTCCTA CAGTGGTGCA GCCTTTTGTG GAATCTAACC TTTTGGTTCC TGTGGGCAGT 
ATAGGAGGAC AGGTTCAAGT GTCCCAGCCA GCAGTGAGTT TAGCACAACA AGCCCCCACT 
ACATCCTCCC AGCAAGCAGC TCTGGAGCAG AATGAAATGC CAATGTGGGG GCCTTGTTGC 
CACTGGCTGT TCTGTTGGGA AAGCACTCAG GGAGTCTCTC AAGTTGCTCC TCCAGAGCCA 
GTTCCAGTGG CACAGGCACA GCCTACCACT TTGGTCTCCT CTATAGACAG TGCACATTCA 
GATGTTGCTT CAGGAATGAG TGATGGCAAT GAGAATGTCC CGTCCTCCAG TGGAAGGCAT 
GAAGGGAGAG CTACAAAACG ACATTACCGA AAATCTGTAA GAAGTCGCTC TCGTCATGAA 
AAGACTTCGC GTCCAAAATT AAGAATTTTG AATGTTTCAA ATAAAGGAGA CCGAGTAGTA 
GAATGTCAAC TAGAGACTCA TAATCGGAAA ATGGTTACAT TCAAATTTGA TTTAGATGGT 
GACAATCCTG AGGAGATAGC AACAATTATG GTGAACAATG ACTTTATTCT AGCAATGGAG 
AGAGAGTCCT TTGTGGATCA AGTACGAGAA ATTATTGAAA AGGCTGATGA AATGCTCAGT 
GAGGATGTCA GTGTGGAACC AGAGGGTGAC CAGGGATTGG AGAGTCTGCA GGGAAAGGAT 
GACTATGGCT TTACAGGTTC TCAAAAATTG GAAGGAGAAT TCAAACAACC AGTTCCTGCG 
TCTTCCATGC CACAGCAAAT AGGCATTCCT ACCAGTTCTT TAACTCAAGT TGTTCATTCT 
GCTGGAAGAC GGTTTATAGT GAGTCCTGTG CCAGAAAGTC GATTACGAGA ATCAAAAGTT 
TTCACTAGTG AAATACCAGA TACAGTTGCT GCCTCTACTC ACAGCGCTGG AATGAACTTG 
TCTCACTCTG CTTCATCCCT TAGCCTACAG CAAGCCTTTT CTGAACTTAG ACATGTGCAG 
ATGACAGAAG GGCCCAGCAC AGCACCTCCC AACTTCAGTC ATACAGGACC AACATTTCCA 
GTTGTTCCTC CTTCCATGAG TAGCATTGCT GGAGTCCCAA CCACAGCAGT AGCCACACCT 
TCAGTATCAG TCCCTGCAAC TAGCAGCCCT CTAAATGACA GTTCCACATC AGTAATTCAA 
TCTGAGATTC CAGTGCCCAC TGAAACAGGG GTTGGTGGAG TTCCCACCTG CACAAGTGTA 
ATGCCTTCAA GTGGTCTCCC TGTACCACCC ATATCTGAAT CGCCAATATT TTCCACTATG 
GTTTCAAGCA TCACAGTACC TACAGTTGTC TCAATATCAG CAACATCCCA GCCAGTTCAA 
GCTCCCACAT CTGGGAGCAC TGTTTCTAGC ATAGGAACTT TGCCTTCTAT ACCAGTTTCA 
ACAGCTTTAG CCTCTGCAGT GGGCAGTGTT GCTATCCCAG GTGCTAAGCC TCCACCTGTA 
CTATCACAGC AGGCAGCAGG CAGTACTACT GGGGCAGCCA CATTAACATC CGTTCCTGCC 
ATCACTCCGT TCCCAAGCAT AGCTTCACAG CCATCTCTTC AGCTTAGCAG CAGCACCTCT 
GCTCCAACTC TAGCTGAAAC AGTGGTGGTT AGTGCACACT CACTAGATAA AACATCTCAT 
AGCAGTACAA CTGGATTGGC TTTGTCCCTG TCTGCATCAT CTTCCTCCCC TGGAGCAGGA 
TTATCTAGTT CTATTTCTCA GCCTGGTGGG GTGCATCCTT TGGTTACCCC ATCAGCTATG 
GCTTCTATTC CTGCCCTTCC TCAAACAGCA GGACCTACTT CTACACCTTT ATTGCCCCAA 
GTACTTAGTA TCCCACCCTT GGTACAACCT GTTGCCAGTG TGCCTGCTGT GCAGCAGACA 
CTAATTCATA GTCAGCCTCA ACCAGCTTTA CTTCCCAACC AGCCCCACAC TCACTGTCCT 
GAAATAGATG CTGACTTACA GCCCAAAGCT CCTGGAATTG ATGACATAAA GACTCTAGAG 
GAAAAGCTGC GGTCTCTCTT TAGTGAACCG AGCTCATCTG GAGCCCAACA TACATCGGTC 
TCACTAGAGA CTTCACTGGT AGTAGAGACC ACTGTCACGC CAGGTATCCC AACCACTGCT 
ATTGCACCAA GCAAGCTCAT GACATCTACT ACAAGTACAT GCTTACCACC AACCAGTTTG 
CAATTAGGAA CATCTGGTTT GTCAGTTATA CCAGTGGTCA CGCCTGGGCA GGTTTCTACC 
CCAGTCAGCT ATGTGTCAGC CCCAGGCAGC ACTACATCAG GAGTGAAAGC TGGAACTGCT 
CCATCAAAGC CACCTTTGAC TAAAGCTCCA GTGCTGCCAG TGGGTACTGA ACTTCCAGCT 
GGTACTCCAC CCAGCGAGCA GCTGCCACCT TTTCCAGGAC CTTCACTAAC TCAGTCCCAG 
CAACCTCTGG AAGATCTTGA TGCTCAGTTG AGAAGAACAC TTAGTCCAGA GACTATTGCG 
GTGACATCTA CAATTGGTCC TGTGTCCATG GTGGCTCCAA CAGCAGTCAC AGAAGCAGGA 
GCACAGCCTC AGAAGGATGT TTCTCAGATC ACAGAAGGTC CTGTCCTAGC GACTAGTTCG 
GGAACTGGTG TTTTTAAGAT GGGAAGATTT CAGGTTTCAG TTGCAATGGA TGATGTTCAA 
AAGGAGGGTA AAAATAAGTT AGAAGATGGA AAGTCTGTTC ATTTTGAATC CAGCACTTCA 
GAGTCCTCAG TGCTATCAAG TAGTAGTCCA GAAAGTACCC TGGTGAAGAC AGAGCCTAAT 
GGGATAACCA TCCCTGGCAT CTCATCAGAT ATGCCAGATA GTGCCCACAA AACTCCTGCC 
TCAGAAGCAA AGTCAGAAAC TGGGCAGCCT ACCAAGGTTG GACGTTTTCA GGTGACAACT 
ACAGCAAACA AAGTAGGTCG TTTCTCTGTG TCAAGAACTG AAGACAAGCT CATTGAGGCA 
AAGAAAGAGG GACCAATAGC GTCTCCTCCT TTTATGGATT TGGAACAAGG TGTTCTTCCA 
GCTGTGATAC CAAAGAAAGA AAAGCCTGAA CTGTCAGAGC CTTCACATCT GAACGGACCA 
TCTTCTGACC TGGAGGCTGC CTTCCTGAGC AGGGATGTGG ATGATGGTTC AGGTAGTCCA 
CACTCCCCCC ATCAGCTGAG CTCAAAGAGC CTGCCTATCC AGAATCTAAG TCAAAGCCTT 
AGTAATTCAT TCAACTCCTC TTACATGAGT AGTGACAATG AGTCAGATAT TGAAGATGAA 
GATTTAAAAT TAGAGCTGCG AAGACTACGA GAAAAACATC TTAAAGAGAT TCAAGACCTG 
CAGAGTCGCC AGAAGCATGA AATTGAATCT TTGTATACCA AACTGGGAAA GGTTCCCCCT 
GCTGTCATTA TCCCCCCAGC TGCCCCCCTT TCAGGGAGAA GAAGGCGACC CACTAAAAGC 
AAAGGCAGCA AGTCTAGTCG CAGCAGTTCC TTGGGGAATA AAAGTCCCCA GCTTTCAGGT 
AACCTGTCTG GTCAGAGTGC TGCTTCAGTT TTGCACGCTC AGCAGACCCT CCACCCTCCT 
GGCAACATCC CTGAGACTGG GCCAAATCAG CTGTTACAGC CCCTTAAGCC ATCTCCCTCC 
AGTGACAACC TCTACTCAGC CTTTACCAGT GATGGTGCCA TTTCAGTCCC AAGCCTTTCT 
GCTCCAGGTC AAGGCTGTGC GAAGTTTAAC TGTGCATCTG AGCAGGTGAC ATTCAAACCT 
GGTGGCAGGA GGACCCGATT TCTGAGTACG CCCTGCTTGG CTCTTTGGAA GATGGTGAAA 
AAAGTCTGTC CTTGCAACCA GCTCTGTAGG ACCAGCAGCA CAAACACTGT CGGGGGAACA 
GTGAACAGCC AAGCTGCCCC AGCTCAGCCT CCTGCCATGA CATCCAGCAG GAAGGGCACA 
TTCACAGATG ACCTACACAA GTTGGTAGAC AATTGGGCCC GAGATGCCAT GAATCTCTCA 
GGCAGAAGAG GAAGCAAAGG ACACATGAAT TATGAGGGCC CTGGAATGGC AAGAAAGTTC 
TCCGCACCTG GTCAGCTGTG CATCTCCATG ACCTCAAATC TGGGTGGTTC TGCCCCCATC 
TCTGCAGCAT CAGCTACCTC TCTAGGTCAC TTCACCAAGT CTATGTGCCC CCCACAGCAG 
TACGGTTTTC CAGCTCCCCC CTTTGGCACT CAGTGGAGTG GGACGGGTGG CCCGGCACCA 
CAGCCACTTG GCCAGTTCCA GCCTGTGGGA ACTGCCTCCT TACAGAATTT CAACATCAGC 
AATTTGCAGA AATCCATCAG CAACCCCCCA GGTTCCAACC TGCGGACCAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_022411009.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022266717.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X14, mRNA.

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CloneID	OCe282747
Clone ID Related Accession (Same CDS sequence)	XM_022411009.1
Accession Version	XM_022411009.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase WNK1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG 
CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT 
CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC 
CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA 
GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG 
GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT 
CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA 
CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT 
CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA 
GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA 
TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG 
CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG 
TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT 
CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA 
AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG 
CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT 
GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA 
CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT 
AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA 
GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT 
CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG 
AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT 
ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT 
AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA 
GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT 
AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT 
AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT 
GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT 
GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT 
ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT 
GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG 
GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC 
AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA 
GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA 
GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT 
TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG 
CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT 
CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG 
GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG 
CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT 
AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA 
GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG 
ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG 
TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC 
CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT 
AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG 
CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT 
GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT 
GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT 
TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG 
GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA 
GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT 
AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC 
TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT 
GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT 
TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG 
TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA 
AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC 
TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC 
TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT 
TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA 
AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA 
ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT 
GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC 
AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT 
GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG 
CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC 
TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG 
AAGTTTAACT GTGCATCTGA GCAGGTGACA TTCAAACCTG GTGGCAGGAG GACCCGATTT 
CTGAGGAAGA TGGTGAAAAA AGTCTGTCCT TGCAACCAGC TCTGTATGTG CCCTCCAGCA 
GAGCCGAAAT CAGGCAGGAC ACAGACCCCT TCTTCCTTTC TCATCAAACC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022266717.1
CDS	998..7891
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVGQSQGQPSSSSLTGIPSSLPIQHSQQQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQLPVSQPVPTIQGEPQIPVAAQPSVVPVHSGAHFLPVGQPLSTSLLPQYPVSQIPISTPHASAPQTGFSSLPITMAAGINQPLLTLASSATAAAIPGGPTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPSRLPPQYPGDSNIAPSSSVASICIPSTVLSPPMPTEALATPGYFPTVVQPFVESNLLVPVGSIGGQVQVSQPAVSLAQQAPTTSSQQAALEQNEMPMWGPCCHWLFCWESTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGCAKFNCASEQVTFKPGGRRTRFLRKMVKKVCPCNQLCMCPPAEPKSGRTQTPSSFLIKP

Target ORF information:

RefSeq Version	XM_022411009.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022411009.1

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG 
CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT 
CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC 
CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA 
GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG 
GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT 
CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA 
CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT 
CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA 
GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA 
TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG 
CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG 
TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT 
CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA 
AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG 
CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT 
GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA 
CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT 
AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA 
GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT 
CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG 
AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT 
ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT 
AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA 
GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT 
AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT 
AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT 
GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT 
GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT 
ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT 
GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG 
GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC 
AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA 
GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA 
GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT 
TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG 
CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT 
CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG 
GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG 
CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT 
AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA 
GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG 
ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG 
TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC 
CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT 
AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG 
CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT 
GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT 
GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT 
TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG 
GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA 
GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT 
AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC 
TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT 
GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT 
TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG 
TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA 
AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC 
TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC 
TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT 
TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA 
AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA 
ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT 
GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC 
AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT 
GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG 
CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC 
TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG 
AAGTTTAACT GTGCATCTGA GCAGGTGACA TTCAAACCTG GTGGCAGGAG GACCCGATTT 
CTGAGGAAGA TGGTGAAAAA AGTCTGTCCT TGCAACCAGC TCTGTATGTG CCCTCCAGCA 
GAGCCGAAAT CAGGCAGGAC ACAGACCCCT TCTTCCTTTC TCATCAAACC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_022411010.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022266718.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X16, mRNA.

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CloneID	OCe282748
Clone ID Related Accession (Same CDS sequence)	XM_022411010.1
Accession Version	XM_022411010.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase WNK1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGAGCAC TCAGGGAGTC 
TCTCAAGTTG CTCCTCCAGA GCCAGTTCCA GTGGCACAGG CACAGCCTAC CACTTTGGTC 
TCCTCTATAG ACAGTGCACA TTCAGATGTT GCTTCAGGAA TGAGTGATGG CAATGAGAAT 
GTCCCGTCCT CCAGTGGAAG GCATGAAGGG AGAGCTACAA AACGACATTA CCGAAAATCT 
GTAAGAAGTC GCTCTCGTCA TGAAAAGACT TCGCGTCCAA AATTAAGAAT TTTGAATGTT 
TCAAATAAAG GAGACCGAGT AGTAGAATGT CAACTAGAGA CTCATAATCG GAAAATGGTT 
ACATTCAAAT TTGATTTAGA TGGTGACAAT CCTGAGGAGA TAGCAACAAT TATGGTGAAC 
AATGACTTTA TTCTAGCAAT GGAGAGAGAG TCCTTTGTGG ATCAAGTACG AGAAATTATT 
GAAAAGGCTG ATGAAATGCT CAGTGAGGAT GTCAGTGTGG AACCAGAGGG TGACCAGGGA 
TTGGAGAGTC TGCAGGGAAA GGATGACTAT GGCTTTACAG GTTCTCAAAA ATTGGAAGGA 
GAATTCAAAC AACCAGTTCC TGCGTCTTCC ATGCCACAGC AAATAGGCAT TCCTACCAGT 
TCTTTAACTC AAGTTGTTCA TTCTGCTGGA AGACGGTTTA TAGTGAGTCC TGTGCCAGAA 
AGTCGATTAC GAGAATCAAA AGTTTTCACT AGTGAAATAC CAGATACAGT TGCTGCCTCT 
ACTCACAGCG CTGGAATGAA CTTGTCTCAC TCTGCTTCAT CCCTTAGCCT ACAGCAAGCC 
TTTTCTGAAC TTAGACATGT GCAGATGACA GAAGGGCCCA GCACAGCACC TCCCAACTTC 
AGTCATACAG GACCAACATT TCCAGTTGTT CCTCCTTCCA TGAGTAGCAT TGCTGGAGTC 
CCAACCACAG CAGTAGCCAC ACCTTCAGTA TCAGTCCCTG CAACTAGCAG CCCTCTAAAT 
GACAGTTCCA CATCAGTAAT TCAATCTGAG ATTCCAGTGC CCACTGAAAC AGGGGTTGGT 
GGAGTTCCCA CCTGCACAAG TGTAATGCCT TCAAGTGGTC TCCCTGTACC ACCCATATCT 
GAATCGCCAA TATTTTCCAC TATGGTTTCA AGCATCACAG TACCTACAGT TGTCTCAATA 
TCAGCAACAT CCCAGCCAGT TCAAGCTCCC ACATCTGGGA GCACTGTTTC TAGCATAGGA 
ACTTTGCCTT CTATACCAGT TTCAACAGCT TTAGCCTCTG CAGTGGGCAG TGTTGCTATC 
CCAGGTGCTA AGCCTCCACC TGTACTATCA CAGCAGGCAG CAGGCAGTAC TACTGGGGCA 
GCCACATTAA CATCCGTTCC TGCCATCACT CCGTTCCCAA GCATAGCTTC ACAGCCATCT 
CTTCAGCTTA GCAGCAGCAC CTCTGCTCCA ACTCTAGCTG AAACAGTGGT GGTTAGTGCA 
CACTCACTAG ATAAAACATC TCATAGCAGT ACAACTGGAT TGGCTTTGTC CCTGTCTGCA 
TCATCTTCCT CCCCTGGAGC AGGATTATCT AGTTCTATTT CTCAGCCTGG TGGGGTGCAT 
CCTTTGGTTA CCCCATCAGC TATGGCTTCT ATTCCTGCCC TTCCTCAAAC AGCAGGACCT 
ACTTCTACAC CTTTATTGCC CCAAGTACTT AGTATCCCAC CCTTGGTACA ACCTGTTGCC 
AGTGTGCCTG CTGTGCAGCA GACACTAATT CATAGTCAGC CTCAACCAGC TTTACTTCCC 
AACCAGCCCC ACACTCACTG TCCTGAAATA GATGCTGACT TACAGCCCAA AGCTCCTGGA 
ATTGATGACA TAAAGACTCT AGAGGAAAAG CTGCGGTCTC TCTTTAGTGA ACCGAGCTCA 
TCTGGAGCCC AACATACATC GGTCTCACTA GAGACTTCAC TGGTAGTAGA GACCACTGTC 
ACGCCAGGTA TCCCAACCAC TGCTATTGCA CCAAGCAAGC TCATGACATC TACTACAAGT 
ACATGCTTAC CACCAACCAG TTTGCAATTA GGAACATCTG GTTTGTCAGT TATACCAGTG 
GTCACGCCTG GGCAGGTTTC TACCCCAGTC AGCTATGTGT CAGCCCCAGG CAGCACTACA 
TCAGGAGTGA AAGCTGGAAC TGCTCCATCA AAGCCACCTT TGACTAAAGC TCCAGTGCTG 
CCAGTGGGTA CTGAACTTCC AGCTGGTACT CCACCCAGCG AGCAGCTGCC ACCTTTTCCA 
GGACCTTCAC TAACTCAGTC CCAGCAACCT CTGGAAGATC TTGATGCTCA GTTGAGAAGA 
ACACTTAGTC CAGAGACTAT TGCGGTGACA TCTACAATTG GTCCTGTGTC CATGGTGGCT 
CCAACAGCAG TCACAGAAGC AGGAGCACAG CCTCAGAAGG ATGTTTCTCA GATCACAGAA 
GGTCCTGTCC TAGCGACTAG TTCGGGAACT GGTGTTTTTA AGATGGGAAG ATTTCAGGTT 
TCAGTTGCAA TGGATGATGT TCAAAAGGAG GGTAAAAATA AGTTAGAAGA TGGAAAGTCT 
GTTCATTTTG AATCCAGCAC TTCAGAGTCC TCAGTGCTAT CAAGTAGTAG TCCAGAAAGT 
ACCCTGGTGA AGACAGAGCC TAATGGGATA ACCATCCCTG GCATCTCATC AGATATGCCA 
GATAGTGCCC ACAAAACTCC TGCCTCAGAA GCAAAGTCAG AAACTGGGCA GCCTACCAAG 
GTTGGACGTT TTCAGGTGAC AACTACAGCA AACAAAGTAG GTCGTTTCTC TGTGTCAAGA 
ACTGAAGACA AGCTCATTGA GGCAAAGAAA GAGGGACCAA TAGCGTCTCC TCCTTTTATG 
GATTTGGAAC AAGGTGTTCT TCCAGCTGTG ATACCAAAGA AAGAAAAGCC TGAACTGTCA 
GAGCCTTCAC ATCTGAACGG ACCATCTTCT GACCTGGAGG CTGCCTTCCT GAGCAGGGAT 
GTGGATGATG GTTCAGGTAG TCCACACTCC CCCCATCAGC TGAGCTCAAA GAGCCTGCCT 
ATCCAGAATC TAAGTCAAAG CCTTAGTAAT TCATTCAACT CCTCTTACAT GAGTAGTGAC 
AATGAGTCAG ATATTGAAGA TGAAGATTTA AAATTAGAGC TGCGAAGACT ACGAGAAAAA 
CATCTTAAAG AGATTCAAGA CCTGCAGAGT CGCCAGAAGC ATGAAATTGA ATCTTTGTAT 
ACCAAACTGG GAAAGGTTCC CCCTGCTGTC ATTATCCCCC CAGCTGCCCC CCTTTCAGGG 
AGAAGAAGGC GACCCACTAA AAGCAAAGGC AGCAAGTCTA GTCGCAGCAG TTCCTTGGGG 
AATAAAAGTC CCCAGCTTTC AGGTAACCTG TCTGGTCAGA GTGCTGCTTC AGTTTTGCAC 
GCTCAGCAGA CCCTCCACCC TCCTGGCAAC ATCCCTGAGA CTGGGCCAAA TCAGCTGTTA 
CAGCCCCTTA AGCCATCTCC CTCCAGTGAC AACCTCTACT CAGCCTTTAC CAGTGATGGT 
GCCATTTCAG TCCCAAGCCT TTCTGCTCCA GGTCAAGGCT GTGCGAAGTT TAACTGTGCA 
TCTGAGCAGG TGACATTCAA ACCTGGTGGC AGGAGGACCC GATTTCTGAG TACGCCCTGC 
TTGGCTCTTT GGAAGATGGT GAAAAAAGTC TGTCCTTGCA ACCAGCTCTG TAGGACCAGC 
AGCACAAACA CTGTCGGGGG AACAGTGAAC AGCCAAGCTG CCCCAGCTCA GCCTCCTGCC 
ATGACATCCA GCAGGAAGGG CACATTCACA GATGACCTAC ACAAGTTGGT AGACAATTGG 
GCCCGAGATG CCATGAATCT CTCAGGCAGA AGAGGAAGCA AAGGACACAT GAATTATGAG 
GGCCCTGGAA TGGCAAGAAA GTTCTCCGCA CCTGGTCAGC TGTGCATCTC CATGACCTCA 
AATCTGGGTG GTTCTGCCCC CATCTCTGCA GCATCAGCTA CCTCTCTAGG TCACTTCACC 
AAGTCTATGT GCCCCCCACA GCAGTACGGT TTTCCAGCTC CCCCCTTTGG CACTCAGTGG 
AGTGGGACGG GTGGCCCGGC ACCACAGCCA CTTGGCCAGT TCCAGCCTGT GGGAACTGCC 
TCCTTACAGA ATTTCAACAT CAGCAATTTG CAGAAATCCA TCAGCAACCC CCCAGGTTCC 
AACCTGCGGA CCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022266718.1
CDS	995..7552
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVGQSQGQPSSSSLTGIPSSLPIQHSQQQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQSTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGCAKFNCASEQVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_022411010.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022411010.1

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGAGCAC TCAGGGAGTC 
TCTCAAGTTG CTCCTCCAGA GCCAGTTCCA GTGGCACAGG CACAGCCTAC CACTTTGGTC 
TCCTCTATAG ACAGTGCACA TTCAGATGTT GCTTCAGGAA TGAGTGATGG CAATGAGAAT 
GTCCCGTCCT CCAGTGGAAG GCATGAAGGG AGAGCTACAA AACGACATTA CCGAAAATCT 
GTAAGAAGTC GCTCTCGTCA TGAAAAGACT TCGCGTCCAA AATTAAGAAT TTTGAATGTT 
TCAAATAAAG GAGACCGAGT AGTAGAATGT CAACTAGAGA CTCATAATCG GAAAATGGTT 
ACATTCAAAT TTGATTTAGA TGGTGACAAT CCTGAGGAGA TAGCAACAAT TATGGTGAAC 
AATGACTTTA TTCTAGCAAT GGAGAGAGAG TCCTTTGTGG ATCAAGTACG AGAAATTATT 
GAAAAGGCTG ATGAAATGCT CAGTGAGGAT GTCAGTGTGG AACCAGAGGG TGACCAGGGA 
TTGGAGAGTC TGCAGGGAAA GGATGACTAT GGCTTTACAG GTTCTCAAAA ATTGGAAGGA 
GAATTCAAAC AACCAGTTCC TGCGTCTTCC ATGCCACAGC AAATAGGCAT TCCTACCAGT 
TCTTTAACTC AAGTTGTTCA TTCTGCTGGA AGACGGTTTA TAGTGAGTCC TGTGCCAGAA 
AGTCGATTAC GAGAATCAAA AGTTTTCACT AGTGAAATAC CAGATACAGT TGCTGCCTCT 
ACTCACAGCG CTGGAATGAA CTTGTCTCAC TCTGCTTCAT CCCTTAGCCT ACAGCAAGCC 
TTTTCTGAAC TTAGACATGT GCAGATGACA GAAGGGCCCA GCACAGCACC TCCCAACTTC 
AGTCATACAG GACCAACATT TCCAGTTGTT CCTCCTTCCA TGAGTAGCAT TGCTGGAGTC 
CCAACCACAG CAGTAGCCAC ACCTTCAGTA TCAGTCCCTG CAACTAGCAG CCCTCTAAAT 
GACAGTTCCA CATCAGTAAT TCAATCTGAG ATTCCAGTGC CCACTGAAAC AGGGGTTGGT 
GGAGTTCCCA CCTGCACAAG TGTAATGCCT TCAAGTGGTC TCCCTGTACC ACCCATATCT 
GAATCGCCAA TATTTTCCAC TATGGTTTCA AGCATCACAG TACCTACAGT TGTCTCAATA 
TCAGCAACAT CCCAGCCAGT TCAAGCTCCC ACATCTGGGA GCACTGTTTC TAGCATAGGA 
ACTTTGCCTT CTATACCAGT TTCAACAGCT TTAGCCTCTG CAGTGGGCAG TGTTGCTATC 
CCAGGTGCTA AGCCTCCACC TGTACTATCA CAGCAGGCAG CAGGCAGTAC TACTGGGGCA 
GCCACATTAA CATCCGTTCC TGCCATCACT CCGTTCCCAA GCATAGCTTC ACAGCCATCT 
CTTCAGCTTA GCAGCAGCAC CTCTGCTCCA ACTCTAGCTG AAACAGTGGT GGTTAGTGCA 
CACTCACTAG ATAAAACATC TCATAGCAGT ACAACTGGAT TGGCTTTGTC CCTGTCTGCA 
TCATCTTCCT CCCCTGGAGC AGGATTATCT AGTTCTATTT CTCAGCCTGG TGGGGTGCAT 
CCTTTGGTTA CCCCATCAGC TATGGCTTCT ATTCCTGCCC TTCCTCAAAC AGCAGGACCT 
ACTTCTACAC CTTTATTGCC CCAAGTACTT AGTATCCCAC CCTTGGTACA ACCTGTTGCC 
AGTGTGCCTG CTGTGCAGCA GACACTAATT CATAGTCAGC CTCAACCAGC TTTACTTCCC 
AACCAGCCCC ACACTCACTG TCCTGAAATA GATGCTGACT TACAGCCCAA AGCTCCTGGA 
ATTGATGACA TAAAGACTCT AGAGGAAAAG CTGCGGTCTC TCTTTAGTGA ACCGAGCTCA 
TCTGGAGCCC AACATACATC GGTCTCACTA GAGACTTCAC TGGTAGTAGA GACCACTGTC 
ACGCCAGGTA TCCCAACCAC TGCTATTGCA CCAAGCAAGC TCATGACATC TACTACAAGT 
ACATGCTTAC CACCAACCAG TTTGCAATTA GGAACATCTG GTTTGTCAGT TATACCAGTG 
GTCACGCCTG GGCAGGTTTC TACCCCAGTC AGCTATGTGT CAGCCCCAGG CAGCACTACA 
TCAGGAGTGA AAGCTGGAAC TGCTCCATCA AAGCCACCTT TGACTAAAGC TCCAGTGCTG 
CCAGTGGGTA CTGAACTTCC AGCTGGTACT CCACCCAGCG AGCAGCTGCC ACCTTTTCCA 
GGACCTTCAC TAACTCAGTC CCAGCAACCT CTGGAAGATC TTGATGCTCA GTTGAGAAGA 
ACACTTAGTC CAGAGACTAT TGCGGTGACA TCTACAATTG GTCCTGTGTC CATGGTGGCT 
CCAACAGCAG TCACAGAAGC AGGAGCACAG CCTCAGAAGG ATGTTTCTCA GATCACAGAA 
GGTCCTGTCC TAGCGACTAG TTCGGGAACT GGTGTTTTTA AGATGGGAAG ATTTCAGGTT 
TCAGTTGCAA TGGATGATGT TCAAAAGGAG GGTAAAAATA AGTTAGAAGA TGGAAAGTCT 
GTTCATTTTG AATCCAGCAC TTCAGAGTCC TCAGTGCTAT CAAGTAGTAG TCCAGAAAGT 
ACCCTGGTGA AGACAGAGCC TAATGGGATA ACCATCCCTG GCATCTCATC AGATATGCCA 
GATAGTGCCC ACAAAACTCC TGCCTCAGAA GCAAAGTCAG AAACTGGGCA GCCTACCAAG 
GTTGGACGTT TTCAGGTGAC AACTACAGCA AACAAAGTAG GTCGTTTCTC TGTGTCAAGA 
ACTGAAGACA AGCTCATTGA GGCAAAGAAA GAGGGACCAA TAGCGTCTCC TCCTTTTATG 
GATTTGGAAC AAGGTGTTCT TCCAGCTGTG ATACCAAAGA AAGAAAAGCC TGAACTGTCA 
GAGCCTTCAC ATCTGAACGG ACCATCTTCT GACCTGGAGG CTGCCTTCCT GAGCAGGGAT 
GTGGATGATG GTTCAGGTAG TCCACACTCC CCCCATCAGC TGAGCTCAAA GAGCCTGCCT 
ATCCAGAATC TAAGTCAAAG CCTTAGTAAT TCATTCAACT CCTCTTACAT GAGTAGTGAC 
AATGAGTCAG ATATTGAAGA TGAAGATTTA AAATTAGAGC TGCGAAGACT ACGAGAAAAA 
CATCTTAAAG AGATTCAAGA CCTGCAGAGT CGCCAGAAGC ATGAAATTGA ATCTTTGTAT 
ACCAAACTGG GAAAGGTTCC CCCTGCTGTC ATTATCCCCC CAGCTGCCCC CCTTTCAGGG 
AGAAGAAGGC GACCCACTAA AAGCAAAGGC AGCAAGTCTA GTCGCAGCAG TTCCTTGGGG 
AATAAAAGTC CCCAGCTTTC AGGTAACCTG TCTGGTCAGA GTGCTGCTTC AGTTTTGCAC 
GCTCAGCAGA CCCTCCACCC TCCTGGCAAC ATCCCTGAGA CTGGGCCAAA TCAGCTGTTA 
CAGCCCCTTA AGCCATCTCC CTCCAGTGAC AACCTCTACT CAGCCTTTAC CAGTGATGGT 
GCCATTTCAG TCCCAAGCCT TTCTGCTCCA GGTCAAGGCT GTGCGAAGTT TAACTGTGCA 
TCTGAGCAGG TGACATTCAA ACCTGGTGGC AGGAGGACCC GATTTCTGAG TACGCCCTGC 
TTGGCTCTTT GGAAGATGGT GAAAAAAGTC TGTCCTTGCA ACCAGCTCTG TAGGACCAGC 
AGCACAAACA CTGTCGGGGG AACAGTGAAC AGCCAAGCTG CCCCAGCTCA GCCTCCTGCC 
ATGACATCCA GCAGGAAGGG CACATTCACA GATGACCTAC ACAAGTTGGT AGACAATTGG 
GCCCGAGATG CCATGAATCT CTCAGGCAGA AGAGGAAGCA AAGGACACAT GAATTATGAG 
GGCCCTGGAA TGGCAAGAAA GTTCTCCGCA CCTGGTCAGC TGTGCATCTC CATGACCTCA 
AATCTGGGTG GTTCTGCCCC CATCTCTGCA GCATCAGCTA CCTCTCTAGG TCACTTCACC 
AAGTCTATGT GCCCCCCACA GCAGTACGGT TTTCCAGCTC CCCCCTTTGG CACTCAGTGG 
AGTGGGACGG GTGGCCCGGC ACCACAGCCA CTTGGCCAGT TCCAGCCTGT GGGAACTGCC 
TCCTTACAGA ATTTCAACAT CAGCAATTTG CAGAAATCCA TCAGCAACCC CCCAGGTTCC 
AACCTGCGGA CCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_022411011.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022266719.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X17, mRNA.

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CloneID	OCe282749
Clone ID Related Accession (Same CDS sequence)	XM_022411011.1
Accession Version	XM_022411011.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase WNK1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGGG AGTACAGCAG ACAGCCCCTC CACAACAGAC AGCGCAGTAT 
TCGCTTCCAC AGACATCAGC CTCCAGTGAG GCCACTATTG CACAACCAGT CAGTCAGTCT 
CAGGCTCCAC AGGTCTTGCC TCAAATATCA GCTGGAAAAC AGAGCACTCA GGGAGTCTCT 
CAAGTTGCTC CTCCAGAGCC AGTTCCAGTG GCACAGGCAC AGCCTACCAC TTTGGTCTCC 
TCTATAGACA GTGCACATTC AGATGTTGCT TCAGGAATGA GTGATGGCAA TGAGAATGTC 
CCGTCCTCCA GTGGAAGGCA TGAAGGGAGA GCTACAAAAC GACATTACCG AAAATCTGTA 
AGAAGTCGCT CTCGTCATGA AAAGACTTCG CGTCCAAAAT TAAGAATTTT GAATGTTTCA 
AATAAAGGAG ACCGAGTAGT AGAATGTCAA CTAGAGACTC ATAATCGGAA AATGGTTACA 
TTCAAATTTG ATTTAGATGG TGACAATCCT GAGGAGATAG CAACAATTAT GGTGAACAAT 
GACTTTATTC TAGCAATGGA GAGAGAGTCC TTTGTGGATC AAGTACGAGA AATTATTGAA 
AAGGCTGATG AAATGCTCAG TGAGGATGTC AGTGTGGAAC CAGAGGGTGA CCAGGGATTG 
GAGAGTCTGC AGGGAAAGGA TGACTATGGC TTTACAGGTT CTCAAAAATT GGAAGGAGAA 
TTCAAACAAC CAGTTCCTGC GTCTTCCATG CCACAGCAAA TAGGCATTCC TACCAGTTCT 
TTAACTCAAG TTGTTCATTC TGCTGGAAGA CGGTTTATAG TGAGTCCTGT GCCAGAAAGT 
CGATTACGAG AATCAAAAGT TTTCACTAGT GAAATACCAG ATACAGTTGC TGCCTCTACT 
CACAGCGCTG GAATGAACTT GTCTCACTCT GCTTCATCCC TTAGCCTACA GCAAGCCTTT 
TCTGAACTTA GACATGTGCA GATGACAGAA GGGCCCAGCA CAGCACCTCC CAACTTCAGT 
CATACAGGAC CAACATTTCC AGTTGTTCCT CCTTCCATGA GTAGCATTGC TGGAGTCCCA 
ACCACAGCAG TAGCCACACC TTCAGTATCA GTCCCTGCAA CTAGCAGCCC TCTAAATGAC 
AGTTCCACAT CAGTAATTCA ATCTGAGATT CCAGTGCCCA CTGAAACAGG GGTTGGTGGA 
GTTCCCACCT GCACAAGTGT AATGCCTTCA AGTGGTCTCC CTGTACCACC CATATCTGAA 
TCGCCAATAT TTTCCACTAT GGTTTCAAGC ATCACAGTAC CTACAGTTGT CTCAATATCA 
GCAACATCCC AGCCAGTTCA AGCTCCCACA TCTGGGAGCA CTGTTTCTAG CATAGGAACT 
TTGCCTTCTA TACCAGTTTC AACAGCTTTA GCCTCTGCAG TGGGCAGTGT TGCTATCCCA 
GGTGCTAAGC CTCCACCTGT ACTATCACAG CAGGCAGCAG GCAGTACTAC TGGGGCAGCC 
ACATTAACAT CCGTTCCTGC CATCACTCCG TTCCCAAGCA TAGCTTCACA GCCATCTCTT 
CAGCTTAGCA GCAGCACCTC TGCTCCAACT CTAGCTGAAA CAGTGGTGGT TAGTGCACAC 
TCACTAGATA AAACATCTCA TAGCAGTACA ACTGGATTGG CTTTGTCCCT GTCTGCATCA 
TCTTCCTCCC CTGGAGCAGG ATTATCTAGT TCTATTTCTC AGCCTGGTGG GGTGCATCCT 
TTGGTTACCC CATCAGCTAT GGCTTCTATT CCTGCCCTTC CTCAAACAGC AGGACCTACT 
TCTACACCTT TATTGCCCCA AGTACTTAGT ATCCCACCCT TGGTACAACC TGTTGCCAGT 
GTGCCTGCTG TGCAGCAGAC ACTAATTCAT AGTCAGCCTC AACCAGCTTT ACTTCCCAAC 
CAGCCCCACA CTCACTGTCC TGAAATAGAT GCTGACTTAC AGCCCAAAGC TCCTGGAATT 
GATGACATAA AGACTCTAGA GGAAAAGCTG CGGTCTCTCT TTAGTGAACC GAGCTCATCT 
GGAGCCCAAC ATACATCGGT CTCACTAGAG ACTTCACTGG TAGTAGAGAC CACTGTCACG 
CCAGGTATCC CAACCACTGC TATTGCACCA AGCAAGCTCA TGACATCTAC TACAAGTACA 
TGCTTACCAC CAACCAGTTT GCAATTAGGA ACATCTGGTT TGTCAGTTAT ACCAGTGGTC 
ACGCCTGGGC AGGTTTCTAC CCCAGTCAGC TATGTGTCAG CCCCAGGCAG CACTACATCA 
GGAGTGAAAG CTGGAACTGC TCCATCAAAG CCACCTTTGA CTAAAGCTCC AGTGCTGCCA 
GTGGGTACTG AACTTCCAGC TGGTACTCCA CCCAGCGAGC AGCTGCCACC TTTTCCAGGA 
CCTTCACTAA CTCAGTCCCA GCAACCTCTG GAAGATCTTG ATGCTCAGTT GAGAAGAACA 
CTTAGTCCAG AGACTATTGC GGTGACATCT ACAATTGGTC CTGTGTCCAT GGTGGCTCCA 
ACAGCAGTCA CAGAAGCAGG AGCACAGCCT CAGAAGGATG TTTCTCAGAT CACAGAAGGT 
CCTGTCCTAG CGACTAGTTC GGGAACTGGT GTTTTTAAGA TGGGAAGATT TCAGGTTTCA 
GTTGCAATGG ATGATGTTCA AAAGGAGGGT AAAAATAAGT TAGAAGATGG AAAGTCTGTT 
CATTTTGAAT CCAGCACTTC AGAGTCCTCA GTGCTATCAA GTAGTAGTCC AGAAAGTACC 
CTGGTGAAGA CAGAGCCTAA TGGGATAACC ATCCCTGGCA TCTCATCAGA TATGCCAGAT 
AGTGCCCACA AAACTCCTGC CTCAGAAGCA AAGTCAGAAA CTGGGCAGCC TACCAAGGTT 
GGACGTTTTC AGGTGACAAC TACAGCAAAC AAAGTAGGTC GTTTCTCTGT GTCAAGAACT 
GAAGACAAGC TCATTGAGGC AAAGAAAGAG GGACCAATAG CGTCTCCTCC TTTTATGGAT 
TTGGAACAAG GTGTTCTTCC AGCTGTGATA CCAAAGAAAG AAAAGCCTGA ACTGTCAGAG 
CCTTCACATC TGAACGGACC ATCTTCTGAC CTGGAGGCTG CCTTCCTGAG CAGGGATGTG 
GATGATGGTT CAGGTAGTCC ACACTCCCCC CATCAGCTGA GCTCAAAGAG CCTGCCTATC 
CAGAATCTAA GTCAAAGCCT TAGTAATTCA TTCAACTCCT CTTACATGAG TAGTGACAAT 
GAGTCAGATA TTGAAGATGA AGATTTAAAA TTAGAGCTGC GAAGACTACG AGAAAAACAT 
CTTAAAGAGA TTCAAGACCT GCAGAGTCGC CAGAAGCATG AAATTGAATC TTTGTATACC 
AAACTGGGAA AGGTTCCCCC TGCTGTCATT ATCCCCCCAG CTGCCCCCCT TTCAGGGAGA 
AGAAGGCGAC CCACTAAAAG CAAAGGCAGC AAGTCTAGTC GCAGCAGTTC CTTGGGGAAT 
AAAAGTCCCC AGCTTTCAGG TAACCTGTCT GGTCAGAGTG CTGCTTCAGT TTTGCACGCT 
CAGCAGACCC TCCACCCTCC TGGCAACATC CCTGAGACTG GGCCAAATCA GCTGTTACAG 
CCCCTTAAGC CATCTCCCTC CAGTGACAAC CTCTACTCAG CCTTTACCAG TGATGGTGCC 
ATTTCAGTCC CAAGCCTTTC TGCTCCAGGT CAAGGCTGTG CGAAGTTTAA CTGTGCATCT 
GAGCAGGTGA CATTCAAACC TGGTGGCAGG AGGACCCGAT TTCTGAGTAC GCCCTGCTTG 
GCTCTTTGGA AGATGGTGAA AAAAGTCTGT CCTTGCAACC AGCTCTGTAG GACCAGCAGC 
ACAAACACTG TCGGGGGAAC AGTGAACAGC CAAGCTGCCC CAGCTCAGCC TCCTGCCATG 
ACATCCAGCA GGAAGGGCAC ATTCACAGAT GACCTACACA AGTTGGTAGA CAATTGGGCC 
CGAGATGCCA TGAATCTCTC AGGCAGAAGA GGAAGCAAAG GACACATGAA TTATGAGGGC 
CCTGGAATGG CAAGAAAGTT CTCCGCACCT GGTCAGCTGT GCATCTCCAT GACCTCAAAT 
CTGGGTGGTT CTGCCCCCAT CTCTGCAGCA TCAGCTACCT CTCTAGGTCA CTTCACCAAG 
TCTATGTGCC CCCCACAGCA GTACGGTTTT CCAGCTCCCC CCTTTGGCAC TCAGTGGAGT 
GGGACGGGTG GCCCGGCACC ACAGCCACTT GGCCAGTTCC AGCCTGTGGG AACTGCCTCC 
TTACAGAATT TCAACATCAG CAATTTGCAG AAATCCATCA GCAACCCCCC AGGTTCCAAC 
CTGCGGACCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022266719.1
CDS	995..7549
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVGQSQGQPSSSSLTGIPSSLPIQHSQQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQSTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGCAKFNCASEQVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_022411011.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022411011.1

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGGG AGTACAGCAG ACAGCCCCTC CACAACAGAC AGCGCAGTAT 
TCGCTTCCAC AGACATCAGC CTCCAGTGAG GCCACTATTG CACAACCAGT CAGTCAGTCT 
CAGGCTCCAC AGGTCTTGCC TCAAATATCA GCTGGAAAAC AGAGCACTCA GGGAGTCTCT 
CAAGTTGCTC CTCCAGAGCC AGTTCCAGTG GCACAGGCAC AGCCTACCAC TTTGGTCTCC 
TCTATAGACA GTGCACATTC AGATGTTGCT TCAGGAATGA GTGATGGCAA TGAGAATGTC 
CCGTCCTCCA GTGGAAGGCA TGAAGGGAGA GCTACAAAAC GACATTACCG AAAATCTGTA 
AGAAGTCGCT CTCGTCATGA AAAGACTTCG CGTCCAAAAT TAAGAATTTT GAATGTTTCA 
AATAAAGGAG ACCGAGTAGT AGAATGTCAA CTAGAGACTC ATAATCGGAA AATGGTTACA 
TTCAAATTTG ATTTAGATGG TGACAATCCT GAGGAGATAG CAACAATTAT GGTGAACAAT 
GACTTTATTC TAGCAATGGA GAGAGAGTCC TTTGTGGATC AAGTACGAGA AATTATTGAA 
AAGGCTGATG AAATGCTCAG TGAGGATGTC AGTGTGGAAC CAGAGGGTGA CCAGGGATTG 
GAGAGTCTGC AGGGAAAGGA TGACTATGGC TTTACAGGTT CTCAAAAATT GGAAGGAGAA 
TTCAAACAAC CAGTTCCTGC GTCTTCCATG CCACAGCAAA TAGGCATTCC TACCAGTTCT 
TTAACTCAAG TTGTTCATTC TGCTGGAAGA CGGTTTATAG TGAGTCCTGT GCCAGAAAGT 
CGATTACGAG AATCAAAAGT TTTCACTAGT GAAATACCAG ATACAGTTGC TGCCTCTACT 
CACAGCGCTG GAATGAACTT GTCTCACTCT GCTTCATCCC TTAGCCTACA GCAAGCCTTT 
TCTGAACTTA GACATGTGCA GATGACAGAA GGGCCCAGCA CAGCACCTCC CAACTTCAGT 
CATACAGGAC CAACATTTCC AGTTGTTCCT CCTTCCATGA GTAGCATTGC TGGAGTCCCA 
ACCACAGCAG TAGCCACACC TTCAGTATCA GTCCCTGCAA CTAGCAGCCC TCTAAATGAC 
AGTTCCACAT CAGTAATTCA ATCTGAGATT CCAGTGCCCA CTGAAACAGG GGTTGGTGGA 
GTTCCCACCT GCACAAGTGT AATGCCTTCA AGTGGTCTCC CTGTACCACC CATATCTGAA 
TCGCCAATAT TTTCCACTAT GGTTTCAAGC ATCACAGTAC CTACAGTTGT CTCAATATCA 
GCAACATCCC AGCCAGTTCA AGCTCCCACA TCTGGGAGCA CTGTTTCTAG CATAGGAACT 
TTGCCTTCTA TACCAGTTTC AACAGCTTTA GCCTCTGCAG TGGGCAGTGT TGCTATCCCA 
GGTGCTAAGC CTCCACCTGT ACTATCACAG CAGGCAGCAG GCAGTACTAC TGGGGCAGCC 
ACATTAACAT CCGTTCCTGC CATCACTCCG TTCCCAAGCA TAGCTTCACA GCCATCTCTT 
CAGCTTAGCA GCAGCACCTC TGCTCCAACT CTAGCTGAAA CAGTGGTGGT TAGTGCACAC 
TCACTAGATA AAACATCTCA TAGCAGTACA ACTGGATTGG CTTTGTCCCT GTCTGCATCA 
TCTTCCTCCC CTGGAGCAGG ATTATCTAGT TCTATTTCTC AGCCTGGTGG GGTGCATCCT 
TTGGTTACCC CATCAGCTAT GGCTTCTATT CCTGCCCTTC CTCAAACAGC AGGACCTACT 
TCTACACCTT TATTGCCCCA AGTACTTAGT ATCCCACCCT TGGTACAACC TGTTGCCAGT 
GTGCCTGCTG TGCAGCAGAC ACTAATTCAT AGTCAGCCTC AACCAGCTTT ACTTCCCAAC 
CAGCCCCACA CTCACTGTCC TGAAATAGAT GCTGACTTAC AGCCCAAAGC TCCTGGAATT 
GATGACATAA AGACTCTAGA GGAAAAGCTG CGGTCTCTCT TTAGTGAACC GAGCTCATCT 
GGAGCCCAAC ATACATCGGT CTCACTAGAG ACTTCACTGG TAGTAGAGAC CACTGTCACG 
CCAGGTATCC CAACCACTGC TATTGCACCA AGCAAGCTCA TGACATCTAC TACAAGTACA 
TGCTTACCAC CAACCAGTTT GCAATTAGGA ACATCTGGTT TGTCAGTTAT ACCAGTGGTC 
ACGCCTGGGC AGGTTTCTAC CCCAGTCAGC TATGTGTCAG CCCCAGGCAG CACTACATCA 
GGAGTGAAAG CTGGAACTGC TCCATCAAAG CCACCTTTGA CTAAAGCTCC AGTGCTGCCA 
GTGGGTACTG AACTTCCAGC TGGTACTCCA CCCAGCGAGC AGCTGCCACC TTTTCCAGGA 
CCTTCACTAA CTCAGTCCCA GCAACCTCTG GAAGATCTTG ATGCTCAGTT GAGAAGAACA 
CTTAGTCCAG AGACTATTGC GGTGACATCT ACAATTGGTC CTGTGTCCAT GGTGGCTCCA 
ACAGCAGTCA CAGAAGCAGG AGCACAGCCT CAGAAGGATG TTTCTCAGAT CACAGAAGGT 
CCTGTCCTAG CGACTAGTTC GGGAACTGGT GTTTTTAAGA TGGGAAGATT TCAGGTTTCA 
GTTGCAATGG ATGATGTTCA AAAGGAGGGT AAAAATAAGT TAGAAGATGG AAAGTCTGTT 
CATTTTGAAT CCAGCACTTC AGAGTCCTCA GTGCTATCAA GTAGTAGTCC AGAAAGTACC 
CTGGTGAAGA CAGAGCCTAA TGGGATAACC ATCCCTGGCA TCTCATCAGA TATGCCAGAT 
AGTGCCCACA AAACTCCTGC CTCAGAAGCA AAGTCAGAAA CTGGGCAGCC TACCAAGGTT 
GGACGTTTTC AGGTGACAAC TACAGCAAAC AAAGTAGGTC GTTTCTCTGT GTCAAGAACT 
GAAGACAAGC TCATTGAGGC AAAGAAAGAG GGACCAATAG CGTCTCCTCC TTTTATGGAT 
TTGGAACAAG GTGTTCTTCC AGCTGTGATA CCAAAGAAAG AAAAGCCTGA ACTGTCAGAG 
CCTTCACATC TGAACGGACC ATCTTCTGAC CTGGAGGCTG CCTTCCTGAG CAGGGATGTG 
GATGATGGTT CAGGTAGTCC ACACTCCCCC CATCAGCTGA GCTCAAAGAG CCTGCCTATC 
CAGAATCTAA GTCAAAGCCT TAGTAATTCA TTCAACTCCT CTTACATGAG TAGTGACAAT 
GAGTCAGATA TTGAAGATGA AGATTTAAAA TTAGAGCTGC GAAGACTACG AGAAAAACAT 
CTTAAAGAGA TTCAAGACCT GCAGAGTCGC CAGAAGCATG AAATTGAATC TTTGTATACC 
AAACTGGGAA AGGTTCCCCC TGCTGTCATT ATCCCCCCAG CTGCCCCCCT TTCAGGGAGA 
AGAAGGCGAC CCACTAAAAG CAAAGGCAGC AAGTCTAGTC GCAGCAGTTC CTTGGGGAAT 
AAAAGTCCCC AGCTTTCAGG TAACCTGTCT GGTCAGAGTG CTGCTTCAGT TTTGCACGCT 
CAGCAGACCC TCCACCCTCC TGGCAACATC CCTGAGACTG GGCCAAATCA GCTGTTACAG 
CCCCTTAAGC CATCTCCCTC CAGTGACAAC CTCTACTCAG CCTTTACCAG TGATGGTGCC 
ATTTCAGTCC CAAGCCTTTC TGCTCCAGGT CAAGGCTGTG CGAAGTTTAA CTGTGCATCT 
GAGCAGGTGA CATTCAAACC TGGTGGCAGG AGGACCCGAT TTCTGAGTAC GCCCTGCTTG 
GCTCTTTGGA AGATGGTGAA AAAAGTCTGT CCTTGCAACC AGCTCTGTAG GACCAGCAGC 
ACAAACACTG TCGGGGGAAC AGTGAACAGC CAAGCTGCCC CAGCTCAGCC TCCTGCCATG 
ACATCCAGCA GGAAGGGCAC ATTCACAGAT GACCTACACA AGTTGGTAGA CAATTGGGCC 
CGAGATGCCA TGAATCTCTC AGGCAGAAGA GGAAGCAAAG GACACATGAA TTATGAGGGC 
CCTGGAATGG CAAGAAAGTT CTCCGCACCT GGTCAGCTGT GCATCTCCAT GACCTCAAAT 
CTGGGTGGTT CTGCCCCCAT CTCTGCAGCA TCAGCTACCT CTCTAGGTCA CTTCACCAAG 
TCTATGTGCC CCCCACAGCA GTACGGTTTT CCAGCTCCCC CCTTTGGCAC TCAGTGGAGT 
GGGACGGGTG GCCCGGCACC ACAGCCACTT GGCCAGTTCC AGCCTGTGGG AACTGCCTCC 
TTACAGAATT TCAACATCAG CAATTTGCAG AAATCCATCA GCAACCCCCC AGGTTCCAAC 
CTGCGGACCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_014108325.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963800.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA.

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CloneID	OCe43570
Clone ID Related Accession (Same CDS sequence)	XM_014108325.1
Accession Version	XM_014108325.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase WNK1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG 
CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT 
CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC 
CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA 
GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG 
GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT 
CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA 
CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT 
CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA 
GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA 
TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG 
CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG 
TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT 
CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA 
AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG 
CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT 
GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA 
CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT 
AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA 
GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT 
CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG 
AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT 
ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT 
AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA 
GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT 
AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT 
AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT 
GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT 
GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT 
ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT 
GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG 
GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC 
AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA 
GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA 
GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT 
TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG 
CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT 
CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG 
GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG 
CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT 
AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA 
GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG 
ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG 
TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC 
CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT 
AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG 
CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT 
GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT 
GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT 
TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG 
GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA 
GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT 
AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC 
TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT 
GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT 
TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG 
TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA 
AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC 
TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC 
TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT 
TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA 
AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA 
ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT 
GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC 
AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT 
GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG 
CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC 
TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG 
AAGTTTAACT GTGCATCTGA GCAGGTGACA TTCAAACCTG GTGGCAGGAG GACCCGATTT 
CTGAGTACGC CCTGCTTGGC TCTTTGGAAG ATGGTGAAAA AAGTCTGTCC TTGCAACCAG 
CTCTGTATGT GCCCTCCAGC AGAGCCGAAA TCAGGGACCA GCAGCACAAA CACTGTCGGG 
GGAACAGTGA ACAGCCAAGC TGCCCCAGCT CAGCCTCCTG CCATGACATC CAGCAGGAAG 
GGCACATTCA CAGATGACCT ACACAAGTTG GTAGACAATT GGGCCCGAGA TGCCATGAAT 
CTCTCAGGCA GAAGAGGAAG CAAAGGACAC ATGAATTATG AGGGCCCTGG AATGGCAAGA 
AAGTTCTCCG CACCTGGTCA GCTGTGCATC TCCATGACCT CAAATCTGGG TGGTTCTGCC 
CCCATCTCTG CAGCATCAGC TACCTCTCTA GGTCACTTCA CCAAGTCTAT GTGCCCCCCA 
CAGCAGTACG GTTTTCCAGC TCCCCCCTTT GGCACTCAGT GGAGTGGGAC GGGTGGCCCG 
GCACCACAGC CACTTGGCCA GTTCCAGCCT GTGGGAACTG CCTCCTTACA GAATTTCAAC 
ATCAGCAATT TGCAGAAATC CATCAGCAAC CCCCCAGGTT CCAACCTGCG GACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963800.1
CDS	99..7478
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVGQSQGQPSSSSLTGIPSSLPIQHSQQQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQLPVSQPVPTIQGEPQIPVAAQPSVVPVHSGAHFLPVGQPLSTSLLPQYPVSQIPISTPHASAPQTGFSSLPITMAAGINQPLLTLASSATAAAIPGGPTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPSRLPPQYPGDSNIAPSSSVASICIPSTVLSPPMPTEALATPGYFPTVVQPFVESNLLVPVGSIGGQVQVSQPAVSLAQQAPTTSSQQAALEQNEMPMWGPCCHWLFCWESTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGCAKFNCASEQVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCMCPPAEPKSGTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_014108325.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108325.1

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG 
CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT 
CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC 
CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA 
GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG 
GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT 
CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA 
CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT 
CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA 
GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA 
TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG 
CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG 
TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT 
CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA 
AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG 
CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT 
GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA 
CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT 
AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA 
GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT 
CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG 
AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT 
ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT 
AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA 
GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT 
AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT 
AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT 
GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT 
GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT 
ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT 
GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG 
GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC 
AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA 
GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA 
GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT 
TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG 
CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT 
CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG 
GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG 
CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT 
AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA 
GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG 
ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG 
TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC 
CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT 
AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG 
CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT 
GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT 
GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT 
TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG 
GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA 
GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT 
AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC 
TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT 
GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT 
TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG 
TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA 
AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC 
TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC 
TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT 
TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA 
AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA 
ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT 
GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC 
AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT 
GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG 
CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC 
TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG 
AAGTTTAACT GTGCATCTGA GCAGGTGACA TTCAAACCTG GTGGCAGGAG GACCCGATTT 
CTGAGTACGC CCTGCTTGGC TCTTTGGAAG ATGGTGAAAA AAGTCTGTCC TTGCAACCAG 
CTCTGTATGT GCCCTCCAGC AGAGCCGAAA TCAGGGACCA GCAGCACAAA CACTGTCGGG 
GGAACAGTGA ACAGCCAAGC TGCCCCAGCT CAGCCTCCTG CCATGACATC CAGCAGGAAG 
GGCACATTCA CAGATGACCT ACACAAGTTG GTAGACAATT GGGCCCGAGA TGCCATGAAT 
CTCTCAGGCA GAAGAGGAAG CAAAGGACAC ATGAATTATG AGGGCCCTGG AATGGCAAGA 
AAGTTCTCCG CACCTGGTCA GCTGTGCATC TCCATGACCT CAAATCTGGG TGGTTCTGCC 
CCCATCTCTG CAGCATCAGC TACCTCTCTA GGTCACTTCA CCAAGTCTAT GTGCCCCCCA 
CAGCAGTACG GTTTTCCAGC TCCCCCCTTT GGCACTCAGT GGAGTGGGAC GGGTGGCCCG 
GCACCACAGC CACTTGGCCA GTTCCAGCCT GTGGGAACTG CCTCCTTACA GAATTTCAAC 
ATCAGCAATT TGCAGAAATC CATCAGCAAC CCCCCAGGTT CCAACCTGCG GACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_014108326.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963801.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X2, mRNA.

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CloneID	OCe43571
Clone ID Related Accession (Same CDS sequence)	XM_014108326.1
Accession Version	XM_014108326.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase WNK1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGGG AGTACAGCAG ACAGCCCCTC CACAACAGAC AGCGCAGTAT 
TCGCTTCCAC AGACATCAGC CTCCAGTGAG GCCACTATTG CACAACCAGT CAGTCAGTCT 
CAGGCTCCAC AGGTCTTGCC TCAAATATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA 
GTACCAACTA TCCAAGGCGA ACCTCAGATC CCAGTTGCGG CACAACCCTC AGTTGTTCCA 
GTCCACTCTG GTGCTCATTT CCTCCCTGTG GGACAGCCAC TCTCTACTTC CTTACTCCCT 
CAGTACCCGG TCTCTCAAAT TCCCATATCA ACTCCTCATG CGTCTGCGCC CCAGACAGGT 
TTTTCATCCC TTCCCATTAC AATGGCAGCT GGCATTAATC AGCCTCTGCT CACCTTGGCT 
TCATCTGCTA CAGCAGCTGC GATCCCAGGG GGACCAACTG TGGTTCCTAG TCAGCTTCCA 
ACCCTTCTGC AGCCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAACT CCTGCAACCA 
GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCTGAGGT TCCACTTCCC 
TCTGGAGATG TTCTGTACCA GGGCTTTCCA TCTCGACTGC CACCACAGTA CCCAGGAGAT 
TCAAATATTG CTCCTTCTTC CAGCGTGGCT TCTATTTGCA TCCCTTCTAC AGTCCTATCC 
CCTCCCATGC CCACAGAAGC ACTGGCTACA CCGGGTTACT TTCCTACAGT GGTGCAGCCT 
TTTGTGGAAT CTAACCTTTT GGTTCCTGTG GGCAGTATAG GAGGACAGGT TCAAGTGTCC 
CAGCCAGCAG TGAGTTTAGC ACAACAAGCC CCCACTACAT CCTCCCAGCA AGCAGCTCTG 
GAGCAGAATG AAATGCCAAT GTGGGGGCCT TGTTGCCACT GGCTGTTCTG TTGGGAAAGC 
ACTCAGGGAG TCTCTCAAGT TGCTCCTCCA GAGCCAGTTC CAGTGGCACA GGCACAGCCT 
ACCACTTTGG TCTCCTCTAT AGACAGTGCA CATTCAGATG TTGCTTCAGG AATGAGTGAT 
GGCAATGAGA ATGTCCCGTC CTCCAGTGGA AGGCATGAAG GGAGAGCTAC AAAACGACAT 
TACCGAAAAT CTGTAAGAAG TCGCTCTCGT CATGAAAAGA CTTCGCGTCC AAAATTAAGA 
ATTTTGAATG TTTCAAATAA AGGAGACCGA GTAGTAGAAT GTCAACTAGA GACTCATAAT 
CGGAAAATGG TTACATTCAA ATTTGATTTA GATGGTGACA ATCCTGAGGA GATAGCAACA 
ATTATGGTGA ACAATGACTT TATTCTAGCA ATGGAGAGAG AGTCCTTTGT GGATCAAGTA 
CGAGAAATTA TTGAAAAGGC TGATGAAATG CTCAGTGAGG ATGTCAGTGT GGAACCAGAG 
GGTGACCAGG GATTGGAGAG TCTGCAGGGA AAGGATGACT ATGGCTTTAC AGGTTCTCAA 
AAATTGGAAG GAGAATTCAA ACAACCAGTT CCTGCGTCTT CCATGCCACA GCAAATAGGC 
ATTCCTACCA GTTCTTTAAC TCAAGTTGTT CATTCTGCTG GAAGACGGTT TATAGTGAGT 
CCTGTGCCAG AAAGTCGATT ACGAGAATCA AAAGTTTTCA CTAGTGAAAT ACCAGATACA 
GTTGCTGCCT CTACTCACAG CGCTGGAATG AACTTGTCTC ACTCTGCTTC ATCCCTTAGC 
CTACAGCAAG CCTTTTCTGA ACTTAGACAT GTGCAGATGA CAGAAGGGCC CAGCACAGCA 
CCTCCCAACT TCAGTCATAC AGGACCAACA TTTCCAGTTG TTCCTCCTTC CATGAGTAGC 
ATTGCTGGAG TCCCAACCAC AGCAGTAGCC ACACCTTCAG TATCAGTCCC TGCAACTAGC 
AGCCCTCTAA ATGACAGTTC CACATCAGTA ATTCAATCTG AGATTCCAGT GCCCACTGAA 
ACAGGGGTTG GTGGAGTTCC CACCTGCACA AGTGTAATGC CTTCAAGTGG TCTCCCTGTA 
CCACCCATAT CTGAATCGCC AATATTTTCC ACTATGGTTT CAAGCATCAC AGTACCTACA 
GTTGTCTCAA TATCAGCAAC ATCCCAGCCA GTTCAAGCTC CCACATCTGG GAGCACTGTT 
TCTAGCATAG GAACTTTGCC TTCTATACCA GTTTCAACAG CTTTAGCCTC TGCAGTGGGC 
AGTGTTGCTA TCCCAGGTGC TAAGCCTCCA CCTGTACTAT CACAGCAGGC AGCAGGCAGT 
ACTACTGGGG CAGCCACATT AACATCCGTT CCTGCCATCA CTCCGTTCCC AAGCATAGCT 
TCACAGCCAT CTCTTCAGCT TAGCAGCAGC ACCTCTGCTC CAACTCTAGC TGAAACAGTG 
GTGGTTAGTG CACACTCACT AGATAAAACA TCTCATAGCA GTACAACTGG ATTGGCTTTG 
TCCCTGTCTG CATCATCTTC CTCCCCTGGA GCAGGATTAT CTAGTTCTAT TTCTCAGCCT 
GGTGGGGTGC ATCCTTTGGT TACCCCATCA GCTATGGCTT CTATTCCTGC CCTTCCTCAA 
ACAGCAGGAC CTACTTCTAC ACCTTTATTG CCCCAAGTAC TTAGTATCCC ACCCTTGGTA 
CAACCTGTTG CCAGTGTGCC TGCTGTGCAG CAGACACTAA TTCATAGTCA GCCTCAACCA 
GCTTTACTTC CCAACCAGCC CCACACTCAC TGTCCTGAAA TAGATGCTGA CTTACAGCCC 
AAAGCTCCTG GAATTGATGA CATAAAGACT CTAGAGGAAA AGCTGCGGTC TCTCTTTAGT 
GAACCGAGCT CATCTGGAGC CCAACATACA TCGGTCTCAC TAGAGACTTC ACTGGTAGTA 
GAGACCACTG TCACGCCAGG TATCCCAACC ACTGCTATTG CACCAAGCAA GCTCATGACA 
TCTACTACAA GTACATGCTT ACCACCAACC AGTTTGCAAT TAGGAACATC TGGTTTGTCA 
GTTATACCAG TGGTCACGCC TGGGCAGGTT TCTACCCCAG TCAGCTATGT GTCAGCCCCA 
GGCAGCACTA CATCAGGAGT GAAAGCTGGA ACTGCTCCAT CAAAGCCACC TTTGACTAAA 
GCTCCAGTGC TGCCAGTGGG TACTGAACTT CCAGCTGGTA CTCCACCCAG CGAGCAGCTG 
CCACCTTTTC CAGGACCTTC ACTAACTCAG TCCCAGCAAC CTCTGGAAGA TCTTGATGCT 
CAGTTGAGAA GAACACTTAG TCCAGAGACT ATTGCGGTGA CATCTACAAT TGGTCCTGTG 
TCCATGGTGG CTCCAACAGC AGTCACAGAA GCAGGAGCAC AGCCTCAGAA GGATGTTTCT 
CAGATCACAG AAGGTCCTGT CCTAGCGACT AGTTCGGGAA CTGGTGTTTT TAAGATGGGA 
AGATTTCAGG TTTCAGTTGC AATGGATGAT GTTCAAAAGG AGGGTAAAAA TAAGTTAGAA 
GATGGAAAGT CTGTTCATTT TGAATCCAGC ACTTCAGAGT CCTCAGTGCT ATCAAGTAGT 
AGTCCAGAAA GTACCCTGGT GAAGACAGAG CCTAATGGGA TAACCATCCC TGGCATCTCA 
TCAGATATGC CAGATAGTGC CCACAAAACT CCTGCCTCAG AAGCAAAGTC AGAAACTGGG 
CAGCCTACCA AGGTTGGACG TTTTCAGGTG ACAACTACAG CAAACAAAGT AGGTCGTTTC 
TCTGTGTCAA GAACTGAAGA CAAGCTCATT GAGGCAAAGA AAGAGGGACC AATAGCGTCT 
CCTCCTTTTA TGGATTTGGA ACAAGGTGTT CTTCCAGCTG TGATACCAAA GAAAGAAAAG 
CCTGAACTGT CAGAGCCTTC ACATCTGAAC GGACCATCTT CTGACCTGGA GGCTGCCTTC 
CTGAGCAGGG ATGTGGATGA TGGTTCAGGT AGTCCACACT CCCCCCATCA GCTGAGCTCA 
AAGAGCCTGC CTATCCAGAA TCTAAGTCAA AGCCTTAGTA ATTCATTCAA CTCCTCTTAC 
ATGAGTAGTG ACAATGAGTC AGATATTGAA GATGAAGATT TAAAATTAGA GCTGCGAAGA 
CTACGAGAAA AACATCTTAA AGAGATTCAA GACCTGCAGA GTCGCCAGAA GCATGAAATT 
GAATCTTTGT ATACCAAACT GGGAAAGGTT CCCCCTGCTG TCATTATCCC CCCAGCTGCC 
CCCCTTTCAG GGAGAAGAAG GCGACCCACT AAAAGCAAAG GCAGCAAGTC TAGTCGCAGC 
AGTTCCTTGG GGAATAAAAG TCCCCAGCTT TCAGGTAACC TGTCTGGTCA GAGTGCTGCT 
TCAGTTTTGC ACGCTCAGCA GACCCTCCAC CCTCCTGGCA ACATCCCTGA GACTGGGCCA 
AATCAGCTGT TACAGCCCCT TAAGCCATCT CCCTCCAGTG ACAACCTCTA CTCAGCCTTT 
ACCAGTGATG GTGCCATTTC AGTCCCAAGC CTTTCTGCTC CAGGTCAAGG CTGTGCGAAG 
TTTAACTGTG CATCTGAGCA GGTGACATTC AAACCTGGTG GCAGGAGGAC CCGATTTCTG 
AGTACGCCCT GCTTGGCTCT TTGGAAGATG GTGAAAAAAG TCTGTCCTTG CAACCAGCTC 
TGTATGTGCC CTCCAGCAGA GCCGAAATCA GGGACCAGCA GCACAAACAC TGTCGGGGGA 
ACAGTGAACA GCCAAGCTGC CCCAGCTCAG CCTCCTGCCA TGACATCCAG CAGGAAGGGC 
ACATTCACAG ATGACCTACA CAAGTTGGTA GACAATTGGG CCCGAGATGC CATGAATCTC 
TCAGGCAGAA GAGGAAGCAA AGGACACATG AATTATGAGG GCCCTGGAAT GGCAAGAAAG 
TTCTCCGCAC CTGGTCAGCT GTGCATCTCC ATGACCTCAA ATCTGGGTGG TTCTGCCCCC 
ATCTCTGCAG CATCAGCTAC CTCTCTAGGT CACTTCACCA AGTCTATGTG CCCCCCACAG 
CAGTACGGTT TTCCAGCTCC CCCCTTTGGC ACTCAGTGGA GTGGGACGGG TGGCCCGGCA 
CCACAGCCAC TTGGCCAGTT CCAGCCTGTG GGAACTGCCT CCTTACAGAA TTTCAACATC 
AGCAATTTGC AGAAATCCAT CAGCAACCCC CCAGGTTCCA ACCTGCGGAC CACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963801.1
CDS	99..7475
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVGQSQGQPSSSSLTGIPSSLPIQHSQQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQLPVSQPVPTIQGEPQIPVAAQPSVVPVHSGAHFLPVGQPLSTSLLPQYPVSQIPISTPHASAPQTGFSSLPITMAAGINQPLLTLASSATAAAIPGGPTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPSRLPPQYPGDSNIAPSSSVASICIPSTVLSPPMPTEALATPGYFPTVVQPFVESNLLVPVGSIGGQVQVSQPAVSLAQQAPTTSSQQAALEQNEMPMWGPCCHWLFCWESTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGCAKFNCASEQVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCMCPPAEPKSGTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_014108326.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108326.1

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGGG AGTACAGCAG ACAGCCCCTC CACAACAGAC AGCGCAGTAT 
TCGCTTCCAC AGACATCAGC CTCCAGTGAG GCCACTATTG CACAACCAGT CAGTCAGTCT 
CAGGCTCCAC AGGTCTTGCC TCAAATATCA GCTGGAAAAC AGCTTCCAGT TTCCCAGCCA 
GTACCAACTA TCCAAGGCGA ACCTCAGATC CCAGTTGCGG CACAACCCTC AGTTGTTCCA 
GTCCACTCTG GTGCTCATTT CCTCCCTGTG GGACAGCCAC TCTCTACTTC CTTACTCCCT 
CAGTACCCGG TCTCTCAAAT TCCCATATCA ACTCCTCATG CGTCTGCGCC CCAGACAGGT 
TTTTCATCCC TTCCCATTAC AATGGCAGCT GGCATTAATC AGCCTCTGCT CACCTTGGCT 
TCATCTGCTA CAGCAGCTGC GATCCCAGGG GGACCAACTG TGGTTCCTAG TCAGCTTCCA 
ACCCTTCTGC AGCCTGTGAC TCAGCTGCCA AGTCAGGTTC ACCCACAACT CCTGCAACCA 
GCAGTTCAGT CCATGGGAAT ACCAGCTAAC CTTGGACAAG CTGCTGAGGT TCCACTTCCC 
TCTGGAGATG TTCTGTACCA GGGCTTTCCA TCTCGACTGC CACCACAGTA CCCAGGAGAT 
TCAAATATTG CTCCTTCTTC CAGCGTGGCT TCTATTTGCA TCCCTTCTAC AGTCCTATCC 
CCTCCCATGC CCACAGAAGC ACTGGCTACA CCGGGTTACT TTCCTACAGT GGTGCAGCCT 
TTTGTGGAAT CTAACCTTTT GGTTCCTGTG GGCAGTATAG GAGGACAGGT TCAAGTGTCC 
CAGCCAGCAG TGAGTTTAGC ACAACAAGCC CCCACTACAT CCTCCCAGCA AGCAGCTCTG 
GAGCAGAATG AAATGCCAAT GTGGGGGCCT TGTTGCCACT GGCTGTTCTG TTGGGAAAGC 
ACTCAGGGAG TCTCTCAAGT TGCTCCTCCA GAGCCAGTTC CAGTGGCACA GGCACAGCCT 
ACCACTTTGG TCTCCTCTAT AGACAGTGCA CATTCAGATG TTGCTTCAGG AATGAGTGAT 
GGCAATGAGA ATGTCCCGTC CTCCAGTGGA AGGCATGAAG GGAGAGCTAC AAAACGACAT 
TACCGAAAAT CTGTAAGAAG TCGCTCTCGT CATGAAAAGA CTTCGCGTCC AAAATTAAGA 
ATTTTGAATG TTTCAAATAA AGGAGACCGA GTAGTAGAAT GTCAACTAGA GACTCATAAT 
CGGAAAATGG TTACATTCAA ATTTGATTTA GATGGTGACA ATCCTGAGGA GATAGCAACA 
ATTATGGTGA ACAATGACTT TATTCTAGCA ATGGAGAGAG AGTCCTTTGT GGATCAAGTA 
CGAGAAATTA TTGAAAAGGC TGATGAAATG CTCAGTGAGG ATGTCAGTGT GGAACCAGAG 
GGTGACCAGG GATTGGAGAG TCTGCAGGGA AAGGATGACT ATGGCTTTAC AGGTTCTCAA 
AAATTGGAAG GAGAATTCAA ACAACCAGTT CCTGCGTCTT CCATGCCACA GCAAATAGGC 
ATTCCTACCA GTTCTTTAAC TCAAGTTGTT CATTCTGCTG GAAGACGGTT TATAGTGAGT 
CCTGTGCCAG AAAGTCGATT ACGAGAATCA AAAGTTTTCA CTAGTGAAAT ACCAGATACA 
GTTGCTGCCT CTACTCACAG CGCTGGAATG AACTTGTCTC ACTCTGCTTC ATCCCTTAGC 
CTACAGCAAG CCTTTTCTGA ACTTAGACAT GTGCAGATGA CAGAAGGGCC CAGCACAGCA 
CCTCCCAACT TCAGTCATAC AGGACCAACA TTTCCAGTTG TTCCTCCTTC CATGAGTAGC 
ATTGCTGGAG TCCCAACCAC AGCAGTAGCC ACACCTTCAG TATCAGTCCC TGCAACTAGC 
AGCCCTCTAA ATGACAGTTC CACATCAGTA ATTCAATCTG AGATTCCAGT GCCCACTGAA 
ACAGGGGTTG GTGGAGTTCC CACCTGCACA AGTGTAATGC CTTCAAGTGG TCTCCCTGTA 
CCACCCATAT CTGAATCGCC AATATTTTCC ACTATGGTTT CAAGCATCAC AGTACCTACA 
GTTGTCTCAA TATCAGCAAC ATCCCAGCCA GTTCAAGCTC CCACATCTGG GAGCACTGTT 
TCTAGCATAG GAACTTTGCC TTCTATACCA GTTTCAACAG CTTTAGCCTC TGCAGTGGGC 
AGTGTTGCTA TCCCAGGTGC TAAGCCTCCA CCTGTACTAT CACAGCAGGC AGCAGGCAGT 
ACTACTGGGG CAGCCACATT AACATCCGTT CCTGCCATCA CTCCGTTCCC AAGCATAGCT 
TCACAGCCAT CTCTTCAGCT TAGCAGCAGC ACCTCTGCTC CAACTCTAGC TGAAACAGTG 
GTGGTTAGTG CACACTCACT AGATAAAACA TCTCATAGCA GTACAACTGG ATTGGCTTTG 
TCCCTGTCTG CATCATCTTC CTCCCCTGGA GCAGGATTAT CTAGTTCTAT TTCTCAGCCT 
GGTGGGGTGC ATCCTTTGGT TACCCCATCA GCTATGGCTT CTATTCCTGC CCTTCCTCAA 
ACAGCAGGAC CTACTTCTAC ACCTTTATTG CCCCAAGTAC TTAGTATCCC ACCCTTGGTA 
CAACCTGTTG CCAGTGTGCC TGCTGTGCAG CAGACACTAA TTCATAGTCA GCCTCAACCA 
GCTTTACTTC CCAACCAGCC CCACACTCAC TGTCCTGAAA TAGATGCTGA CTTACAGCCC 
AAAGCTCCTG GAATTGATGA CATAAAGACT CTAGAGGAAA AGCTGCGGTC TCTCTTTAGT 
GAACCGAGCT CATCTGGAGC CCAACATACA TCGGTCTCAC TAGAGACTTC ACTGGTAGTA 
GAGACCACTG TCACGCCAGG TATCCCAACC ACTGCTATTG CACCAAGCAA GCTCATGACA 
TCTACTACAA GTACATGCTT ACCACCAACC AGTTTGCAAT TAGGAACATC TGGTTTGTCA 
GTTATACCAG TGGTCACGCC TGGGCAGGTT TCTACCCCAG TCAGCTATGT GTCAGCCCCA 
GGCAGCACTA CATCAGGAGT GAAAGCTGGA ACTGCTCCAT CAAAGCCACC TTTGACTAAA 
GCTCCAGTGC TGCCAGTGGG TACTGAACTT CCAGCTGGTA CTCCACCCAG CGAGCAGCTG 
CCACCTTTTC CAGGACCTTC ACTAACTCAG TCCCAGCAAC CTCTGGAAGA TCTTGATGCT 
CAGTTGAGAA GAACACTTAG TCCAGAGACT ATTGCGGTGA CATCTACAAT TGGTCCTGTG 
TCCATGGTGG CTCCAACAGC AGTCACAGAA GCAGGAGCAC AGCCTCAGAA GGATGTTTCT 
CAGATCACAG AAGGTCCTGT CCTAGCGACT AGTTCGGGAA CTGGTGTTTT TAAGATGGGA 
AGATTTCAGG TTTCAGTTGC AATGGATGAT GTTCAAAAGG AGGGTAAAAA TAAGTTAGAA 
GATGGAAAGT CTGTTCATTT TGAATCCAGC ACTTCAGAGT CCTCAGTGCT ATCAAGTAGT 
AGTCCAGAAA GTACCCTGGT GAAGACAGAG CCTAATGGGA TAACCATCCC TGGCATCTCA 
TCAGATATGC CAGATAGTGC CCACAAAACT CCTGCCTCAG AAGCAAAGTC AGAAACTGGG 
CAGCCTACCA AGGTTGGACG TTTTCAGGTG ACAACTACAG CAAACAAAGT AGGTCGTTTC 
TCTGTGTCAA GAACTGAAGA CAAGCTCATT GAGGCAAAGA AAGAGGGACC AATAGCGTCT 
CCTCCTTTTA TGGATTTGGA ACAAGGTGTT CTTCCAGCTG TGATACCAAA GAAAGAAAAG 
CCTGAACTGT CAGAGCCTTC ACATCTGAAC GGACCATCTT CTGACCTGGA GGCTGCCTTC 
CTGAGCAGGG ATGTGGATGA TGGTTCAGGT AGTCCACACT CCCCCCATCA GCTGAGCTCA 
AAGAGCCTGC CTATCCAGAA TCTAAGTCAA AGCCTTAGTA ATTCATTCAA CTCCTCTTAC 
ATGAGTAGTG ACAATGAGTC AGATATTGAA GATGAAGATT TAAAATTAGA GCTGCGAAGA 
CTACGAGAAA AACATCTTAA AGAGATTCAA GACCTGCAGA GTCGCCAGAA GCATGAAATT 
GAATCTTTGT ATACCAAACT GGGAAAGGTT CCCCCTGCTG TCATTATCCC CCCAGCTGCC 
CCCCTTTCAG GGAGAAGAAG GCGACCCACT AAAAGCAAAG GCAGCAAGTC TAGTCGCAGC 
AGTTCCTTGG GGAATAAAAG TCCCCAGCTT TCAGGTAACC TGTCTGGTCA GAGTGCTGCT 
TCAGTTTTGC ACGCTCAGCA GACCCTCCAC CCTCCTGGCA ACATCCCTGA GACTGGGCCA 
AATCAGCTGT TACAGCCCCT TAAGCCATCT CCCTCCAGTG ACAACCTCTA CTCAGCCTTT 
ACCAGTGATG GTGCCATTTC AGTCCCAAGC CTTTCTGCTC CAGGTCAAGG CTGTGCGAAG 
TTTAACTGTG CATCTGAGCA GGTGACATTC AAACCTGGTG GCAGGAGGAC CCGATTTCTG 
AGTACGCCCT GCTTGGCTCT TTGGAAGATG GTGAAAAAAG TCTGTCCTTG CAACCAGCTC 
TGTATGTGCC CTCCAGCAGA GCCGAAATCA GGGACCAGCA GCACAAACAC TGTCGGGGGA 
ACAGTGAACA GCCAAGCTGC CCCAGCTCAG CCTCCTGCCA TGACATCCAG CAGGAAGGGC 
ACATTCACAG ATGACCTACA CAAGTTGGTA GACAATTGGG CCCGAGATGC CATGAATCTC 
TCAGGCAGAA GAGGAAGCAA AGGACACATG AATTATGAGG GCCCTGGAAT GGCAAGAAAG 
TTCTCCGCAC CTGGTCAGCT GTGCATCTCC ATGACCTCAA ATCTGGGTGG TTCTGCCCCC 
ATCTCTGCAG CATCAGCTAC CTCTCTAGGT CACTTCACCA AGTCTATGTG CCCCCCACAG 
CAGTACGGTT TTCCAGCTCC CCCCTTTGGC ACTCAGTGGA GTGGGACGGG TGGCCCGGCA 
CCACAGCCAC TTGGCCAGTT CCAGCCTGTG GGAACTGCCT CCTTACAGAA TTTCAACATC 
AGCAATTTGC AGAAATCCAT CAGCAACCCC CCAGGTTCCA ACCTGCGGAC CACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_014108327.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963802.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X3, mRNA.

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CloneID	OCe43572
Clone ID Related Accession (Same CDS sequence)	XM_014108327.1
Accession Version	XM_014108327.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase WNK1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG 
CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT 
CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC 
CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA 
GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG 
GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT 
CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA 
CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT 
CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA 
GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA 
TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG 
CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG 
TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT 
CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA 
AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG 
CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT 
GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA 
CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT 
AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA 
GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT 
CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG 
AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT 
ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT 
AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA 
GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT 
AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT 
AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT 
GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT 
GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT 
ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT 
GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG 
GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC 
AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA 
GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA 
GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT 
TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG 
CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT 
CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG 
GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG 
CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT 
AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA 
GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG 
ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG 
TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC 
CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT 
AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG 
CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT 
GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT 
GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT 
TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG 
GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA 
GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT 
AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC 
TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT 
GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT 
TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG 
TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA 
AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC 
TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC 
TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT 
TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA 
AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA 
ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT 
GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC 
AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT 
GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG 
CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC 
TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG 
AAGTTTAACT GTGCATCTGA GCAGGTGACA TTCAAACCTG GTGGCAGGAG GACCCGATTT 
CTGAGGAAGA TGGTGAAAAA AGTCTGTCCT TGCAACCAGC TCTGTATGTG CCCTCCAGCA 
GAGCCGAAAT CAGGGACCAG CAGCACAAAC ACTGTCGGGG GAACAGTGAA CAGCCAAGCT 
GCCCCAGCTC AGCCTCCTGC CATGACATCC AGCAGGAAGG GCACATTCAC AGATGACCTA 
CACAAGTTGG TAGACAATTG GGCCCGAGAT GCCATGAATC TCTCAGGCAG AAGAGGAAGC 
AAAGGACACA TGAATTATGA GGGCCCTGGA ATGGCAAGAA AGTTCTCCGC ACCTGGTCAG 
CTGTGCATCT CCATGACCTC AAATCTGGGT GGTTCTGCCC CCATCTCTGC AGCATCAGCT 
ACCTCTCTAG GTCACTTCAC CAAGTCTATG TGCCCCCCAC AGCAGTACGG TTTTCCAGCT 
CCCCCCTTTG GCACTCAGTG GAGTGGGACG GGTGGCCCGG CACCACAGCC ACTTGGCCAG 
TTCCAGCCTG TGGGAACTGC CTCCTTACAG AATTTCAACA TCAGCAATTT GCAGAAATCC 
ATCAGCAACC CCCCAGGTTC CAACCTGCGG ACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963802.1
CDS	99..7457
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVGQSQGQPSSSSLTGIPSSLPIQHSQQQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQLPVSQPVPTIQGEPQIPVAAQPSVVPVHSGAHFLPVGQPLSTSLLPQYPVSQIPISTPHASAPQTGFSSLPITMAAGINQPLLTLASSATAAAIPGGPTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPSRLPPQYPGDSNIAPSSSVASICIPSTVLSPPMPTEALATPGYFPTVVQPFVESNLLVPVGSIGGQVQVSQPAVSLAQQAPTTSSQQAALEQNEMPMWGPCCHWLFCWESTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGCAKFNCASEQVTFKPGGRRTRFLRKMVKKVCPCNQLCMCPPAEPKSGTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_014108327.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108327.1

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG 
CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT 
CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC 
CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA 
GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG 
GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT 
CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA 
CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT 
CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA 
GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA 
TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG 
CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG 
TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT 
CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA 
AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG 
CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT 
GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA 
CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT 
AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA 
GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT 
CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG 
AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT 
ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT 
AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA 
GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT 
AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT 
AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT 
GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT 
GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT 
ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT 
GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG 
GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC 
AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA 
GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA 
GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT 
TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG 
CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT 
CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG 
GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG 
CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT 
AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA 
GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG 
ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG 
TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC 
CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT 
AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG 
CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT 
GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT 
GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT 
TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG 
GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA 
GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT 
AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC 
TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT 
GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT 
TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG 
TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA 
AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC 
TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC 
TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT 
TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA 
AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA 
ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT 
GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC 
AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT 
GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG 
CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC 
TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG 
AAGTTTAACT GTGCATCTGA GCAGGTGACA TTCAAACCTG GTGGCAGGAG GACCCGATTT 
CTGAGGAAGA TGGTGAAAAA AGTCTGTCCT TGCAACCAGC TCTGTATGTG CCCTCCAGCA 
GAGCCGAAAT CAGGGACCAG CAGCACAAAC ACTGTCGGGG GAACAGTGAA CAGCCAAGCT 
GCCCCAGCTC AGCCTCCTGC CATGACATCC AGCAGGAAGG GCACATTCAC AGATGACCTA 
CACAAGTTGG TAGACAATTG GGCCCGAGAT GCCATGAATC TCTCAGGCAG AAGAGGAAGC 
AAAGGACACA TGAATTATGA GGGCCCTGGA ATGGCAAGAA AGTTCTCCGC ACCTGGTCAG 
CTGTGCATCT CCATGACCTC AAATCTGGGT GGTTCTGCCC CCATCTCTGC AGCATCAGCT 
ACCTCTCTAG GTCACTTCAC CAAGTCTATG TGCCCCCCAC AGCAGTACGG TTTTCCAGCT 
CCCCCCTTTG GCACTCAGTG GAGTGGGACG GGTGGCCCGG CACCACAGCC ACTTGGCCAG 
TTCCAGCCTG TGGGAACTGC CTCCTTACAG AATTTCAACA TCAGCAATTT GCAGAAATCC 
ATCAGCAACC CCCCAGGTTC CAACCTGCGG ACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_014108328.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963803.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X3, mRNA.

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CloneID	OCe43573
Clone ID Related Accession (Same CDS sequence)	XM_014108328.2 , XM_014108328.1
Accession Version	XM_014108328.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase WNK1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014108328.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG 
CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT 
CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC 
CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA 
GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG 
GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT 
CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA 
CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT 
CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA 
GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA 
TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG 
CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG 
TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT 
CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA 
AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG 
CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT 
GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA 
CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT 
AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA 
GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT 
CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG 
AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT 
ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT 
AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA 
GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT 
AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT 
AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT 
GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT 
GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT 
ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT 
GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG 
GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC 
AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA 
GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA 
GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT 
TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG 
CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT 
CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG 
GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG 
CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT 
AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA 
GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG 
ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG 
TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC 
CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT 
AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG 
CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT 
GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT 
GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT 
TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG 
GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA 
GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT 
AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC 
TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT 
GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT 
TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG 
TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA 
AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC 
TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC 
TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT 
TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA 
AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA 
ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT 
GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC 
AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT 
GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG 
CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC 
TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG 
AAGTTTAACT GTGCATCTGA GCAGGTGACA TTCAAACCTG GTGGCAGGAG GACCCGATTT 
CTGAGGAAGA TGGTGAAAAA AGTCTGTCCT TGCAACCAGC TCTGTAGGAC CAGCAGCACA 
AACACTGTCG GGGGAACAGT GAACAGCCAA GCTGCCCCAG CTCAGCCTCC TGCCATGACA 
TCCAGCAGGA AGGGCACATT CACAGATGAC CTACACAAGT TGGTAGACAA TTGGGCCCGA 
GATGCCATGA ATCTCTCAGG CAGAAGAGGA AGCAAAGGAC ACATGAATTA TGAGGGCCCT 
GGAATGGCAA GAAAGTTCTC CGCACCTGGT CAGCTGTGCA TCTCCATGAC CTCAAATCTG 
GGTGGTTCTG CCCCCATCTC TGCAGCATCA GCTACCTCTC TAGGTCACTT CACCAAGTCT 
ATGTGCCCCC CACAGCAGTA CGGTTTTCCA GCTCCCCCCT TTGGCACTCA GTGGAGTGGG 
ACGGGTGGCC CGGCACCACA GCCACTTGGC CAGTTCCAGC CTGTGGGAAC TGCCTCCTTA 
CAGAATTTCA ACATCAGCAA TTTGCAGAAA TCCATCAGCA ACCCCCCAGG TTCCAACCTG 
CGGACCACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963803.1
CDS	996..8327
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVGQSQGQPSSSSLTGIPSSLPIQHSQQQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQLPVSQPVPTIQGEPQIPVAAQPSVVPVHSGAHFLPVGQPLSTSLLPQYPVSQIPISTPHASAPQTGFSSLPITMAAGINQPLLTLASSATAAAIPGGPTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPSRLPPQYPGDSNIAPSSSVASICIPSTVLSPPMPTEALATPGYFPTVVQPFVESNLLVPVGSIGGQVQVSQPAVSLAQQAPTTSSQQAALEQNEMPMWGPCCHWLFCWESTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGCAKFNCASEQVTFKPGGRRTRFLRKMVKKVCPCNQLCRTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_014108328.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108328.2

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG 
CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT 
CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC 
CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA 
GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG 
GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT 
CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA 
CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT 
CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA 
GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA 
TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG 
CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG 
TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT 
CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA 
AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG 
CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT 
GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA 
CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT 
AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA 
GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT 
CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG 
AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT 
ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT 
AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA 
GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT 
AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT 
AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT 
GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT 
GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT 
ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT 
GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG 
GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC 
AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA 
GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA 
GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT 
TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG 
CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT 
CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG 
GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG 
CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT 
AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA 
GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG 
ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG 
TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC 
CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT 
AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG 
CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT 
GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT 
GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT 
TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG 
GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA 
GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT 
AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC 
TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT 
GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT 
TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG 
TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA 
AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC 
TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC 
TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT 
TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA 
AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA 
ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT 
GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC 
AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT 
GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG 
CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC 
TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG 
AAGTTTAACT GTGCATCTGA GCAGGTGACA TTCAAACCTG GTGGCAGGAG GACCCGATTT 
CTGAGGAAGA TGGTGAAAAA AGTCTGTCCT TGCAACCAGC TCTGTAGGAC CAGCAGCACA 
AACACTGTCG GGGGAACAGT GAACAGCCAA GCTGCCCCAG CTCAGCCTCC TGCCATGACA 
TCCAGCAGGA AGGGCACATT CACAGATGAC CTACACAAGT TGGTAGACAA TTGGGCCCGA 
GATGCCATGA ATCTCTCAGG CAGAAGAGGA AGCAAAGGAC ACATGAATTA TGAGGGCCCT 
GGAATGGCAA GAAAGTTCTC CGCACCTGGT CAGCTGTGCA TCTCCATGAC CTCAAATCTG 
GGTGGTTCTG CCCCCATCTC TGCAGCATCA GCTACCTCTC TAGGTCACTT CACCAAGTCT 
ATGTGCCCCC CACAGCAGTA CGGTTTTCCA GCTCCCCCCT TTGGCACTCA GTGGAGTGGG 
ACGGGTGGCC CGGCACCACA GCCACTTGGC CAGTTCCAGC CTGTGGGAAC TGCCTCCTTA 
CAGAATTTCA ACATCAGCAA TTTGCAGAAA TCCATCAGCA ACCCCCCAGG TTCCAACCTG 
CGGACCACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_014108328.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963803.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X5, mRNA.

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CloneID	OCe43573
Clone ID Related Accession (Same CDS sequence)	XM_014108328.2 , XM_014108328.1
Accession Version	XM_014108328.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase WNK1 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG 
CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT 
CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC 
CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA 
GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG 
GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT 
CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA 
CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT 
CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA 
GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA 
TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG 
CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG 
TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT 
CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA 
AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG 
CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT 
GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA 
CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT 
AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA 
GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT 
CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG 
AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT 
ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT 
AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA 
GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT 
AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT 
AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT 
GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT 
GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT 
ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT 
GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG 
GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC 
AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA 
GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA 
GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT 
TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG 
CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT 
CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG 
GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG 
CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT 
AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA 
GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG 
ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG 
TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC 
CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT 
AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG 
CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT 
GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT 
GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT 
TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG 
GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA 
GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT 
AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC 
TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT 
GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT 
TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG 
TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA 
AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC 
TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC 
TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT 
TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA 
AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA 
ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT 
GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC 
AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT 
GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG 
CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC 
TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG 
AAGTTTAACT GTGCATCTGA GCAGGTGACA TTCAAACCTG GTGGCAGGAG GACCCGATTT 
CTGAGGAAGA TGGTGAAAAA AGTCTGTCCT TGCAACCAGC TCTGTAGGAC CAGCAGCACA 
AACACTGTCG GGGGAACAGT GAACAGCCAA GCTGCCCCAG CTCAGCCTCC TGCCATGACA 
TCCAGCAGGA AGGGCACATT CACAGATGAC CTACACAAGT TGGTAGACAA TTGGGCCCGA 
GATGCCATGA ATCTCTCAGG CAGAAGAGGA AGCAAAGGAC ACATGAATTA TGAGGGCCCT 
GGAATGGCAA GAAAGTTCTC CGCACCTGGT CAGCTGTGCA TCTCCATGAC CTCAAATCTG 
GGTGGTTCTG CCCCCATCTC TGCAGCATCA GCTACCTCTC TAGGTCACTT CACCAAGTCT 
ATGTGCCCCC CACAGCAGTA CGGTTTTCCA GCTCCCCCCT TTGGCACTCA GTGGAGTGGG 
ACGGGTGGCC CGGCACCACA GCCACTTGGC CAGTTCCAGC CTGTGGGAAC TGCCTCCTTA 
CAGAATTTCA ACATCAGCAA TTTGCAGAAA TCCATCAGCA ACCCCCCAGG TTCCAACCTG 
CGGACCACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963803.1
CDS	99..7430
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVGQSQGQPSSSSLTGIPSSLPIQHSQQQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQLPVSQPVPTIQGEPQIPVAAQPSVVPVHSGAHFLPVGQPLSTSLLPQYPVSQIPISTPHASAPQTGFSSLPITMAAGINQPLLTLASSATAAAIPGGPTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPSRLPPQYPGDSNIAPSSSVASICIPSTVLSPPMPTEALATPGYFPTVVQPFVESNLLVPVGSIGGQVQVSQPAVSLAQQAPTTSSQQAALEQNEMPMWGPCCHWLFCWESTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGCAKFNCASEQVTFKPGGRRTRFLRKMVKKVCPCNQLCRTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_014108328.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108328.1

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG 
CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT 
CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC 
CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA 
GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG 
GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT 
CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA 
CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT 
CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA 
GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA 
TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG 
CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG 
TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT 
CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA 
AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG 
CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT 
GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA 
CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT 
AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA 
GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT 
CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG 
AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT 
ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT 
AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA 
GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT 
AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT 
AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT 
GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT 
GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT 
ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT 
GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG 
GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC 
AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA 
GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA 
GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT 
TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG 
CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT 
CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG 
GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG 
CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT 
AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA 
GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG 
ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG 
TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC 
CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT 
AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG 
CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT 
GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT 
GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT 
TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG 
GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA 
GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT 
AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC 
TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT 
GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT 
TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG 
TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA 
AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC 
TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC 
TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT 
TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA 
AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA 
ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT 
GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC 
AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT 
GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG 
CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC 
TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGCTGTGCG 
AAGTTTAACT GTGCATCTGA GCAGGTGACA TTCAAACCTG GTGGCAGGAG GACCCGATTT 
CTGAGGAAGA TGGTGAAAAA AGTCTGTCCT TGCAACCAGC TCTGTAGGAC CAGCAGCACA 
AACACTGTCG GGGGAACAGT GAACAGCCAA GCTGCCCCAG CTCAGCCTCC TGCCATGACA 
TCCAGCAGGA AGGGCACATT CACAGATGAC CTACACAAGT TGGTAGACAA TTGGGCCCGA 
GATGCCATGA ATCTCTCAGG CAGAAGAGGA AGCAAAGGAC ACATGAATTA TGAGGGCCCT 
GGAATGGCAA GAAAGTTCTC CGCACCTGGT CAGCTGTGCA TCTCCATGAC CTCAAATCTG 
GGTGGTTCTG CCCCCATCTC TGCAGCATCA GCTACCTCTC TAGGTCACTT CACCAAGTCT 
ATGTGCCCCC CACAGCAGTA CGGTTTTCCA GCTCCCCCCT TTGGCACTCA GTGGAGTGGG 
ACGGGTGGCC CGGCACCACA GCCACTTGGC CAGTTCCAGC CTGTGGGAAC TGCCTCCTTA 
CAGAATTTCA ACATCAGCAA TTTGCAGAAA TCCATCAGCA ACCCCCCAGG TTCCAACCTG 
CGGACCACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_014108329.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963804.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X6, mRNA.

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CloneID	OCe43574
Clone ID Related Accession (Same CDS sequence)	XM_014108329.1
Accession Version	XM_014108329.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase WNK1 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG 
CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT 
CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC 
CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA 
GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG 
GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT 
CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA 
CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT 
CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA 
GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA 
TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG 
CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG 
TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT 
CTGGAGAGCA CTCAGGGAGT CTCTCAAGTT GCTCCTCCAG AGCCAGTTCC AGTGGCACAG 
GCACAGCCTA CCACTTTGGT CTCCTCTATA GACAGTGCAC ATTCAGATGT TGCTTCAGGA 
ATGAGTGATG GCAATGAGAA TGTCCCGTCC TCCAGTGGAA GGCATGAAGG GAGAGCTACA 
AAACGACATT ACCGAAAATC TGTAAGAAGT CGCTCTCGTC ATGAAAAGAC TTCGCGTCCA 
AAATTAAGAA TTTTGAATGT TTCAAATAAA GGAGACCGAG TAGTAGAATG TCAACTAGAG 
ACTCATAATC GGAAAATGGT TACATTCAAA TTTGATTTAG ATGGTGACAA TCCTGAGGAG 
ATAGCAACAA TTATGGTGAA CAATGACTTT ATTCTAGCAA TGGAGAGAGA GTCCTTTGTG 
GATCAAGTAC GAGAAATTAT TGAAAAGGCT GATGAAATGC TCAGTGAGGA TGTCAGTGTG 
GAACCAGAGG GTGACCAGGG ATTGGAGAGT CTGCAGGGAA AGGATGACTA TGGCTTTACA 
GGTTCTCAAA AATTGGAAGG AGAATTCAAA CAACCAGTTC CTGCGTCTTC CATGCCACAG 
CAAATAGGCA TTCCTACCAG TTCTTTAACT CAAGTTGTTC ATTCTGCTGG AAGACGGTTT 
ATAGTGAGTC CTGTGCCAGA AAGTCGATTA CGAGAATCAA AAGTTTTCAC TAGTGAAATA 
CCAGATACAG TTGCTGCCTC TACTCACAGC GCTGGAATGA ACTTGTCTCA CTCTGCTTCA 
TCCCTTAGCC TACAGCAAGC CTTTTCTGAA CTTAGACATG TGCAGATGAC AGAAGGGCCC 
AGCACAGCAC CTCCCAACTT CAGTCATACA GGACCAACAT TTCCAGTTGT TCCTCCTTCC 
ATGAGTAGCA TTGCTGGAGT CCCAACCACA GCAGTAGCCA CACCTTCAGT ATCAGTCCCT 
GCAACTAGCA GCCCTCTAAA TGACAGTTCC ACATCAGTAA TTCAATCTGA GATTCCAGTG 
CCCACTGAAA CAGGGGTTGG TGGAGTTCCC ACCTGCACAA GTGTAATGCC TTCAAGTGGT 
CTCCCTGTAC CACCCATATC TGAATCGCCA ATATTTTCCA CTATGGTTTC AAGCATCACA 
GTACCTACAG TTGTCTCAAT ATCAGCAACA TCCCAGCCAG TTCAAGCTCC CACATCTGGG 
AGCACTGTTT CTAGCATAGG AACTTTGCCT TCTATACCAG TTTCAACAGC TTTAGCCTCT 
GCAGTGGGCA GTGTTGCTAT CCCAGGTGCT AAGCCTCCAC CTGTACTATC ACAGCAGGCA 
GCAGGCAGTA CTACTGGGGC AGCCACATTA ACATCCGTTC CTGCCATCAC TCCGTTCCCA 
AGCATAGCTT CACAGCCATC TCTTCAGCTT AGCAGCAGCA CCTCTGCTCC AACTCTAGCT 
GAAACAGTGG TGGTTAGTGC ACACTCACTA GATAAAACAT CTCATAGCAG TACAACTGGA 
TTGGCTTTGT CCCTGTCTGC ATCATCTTCC TCCCCTGGAG CAGGATTATC TAGTTCTATT 
TCTCAGCCTG GTGGGGTGCA TCCTTTGGTT ACCCCATCAG CTATGGCTTC TATTCCTGCC 
CTTCCTCAAA CAGCAGGACC TACTTCTACA CCTTTATTGC CCCAAGTACT TAGTATCCCA 
CCCTTGGTAC AACCTGTTGC CAGTGTGCCT GCTGTGCAGC AGACACTAAT TCATAGTCAG 
CCTCAACCAG CTTTACTTCC CAACCAGCCC CACACTCACT GTCCTGAAAT AGATGCTGAC 
TTACAGCCCA AAGCTCCTGG AATTGATGAC ATAAAGACTC TAGAGGAAAA GCTGCGGTCT 
CTCTTTAGTG AACCGAGCTC ATCTGGAGCC CAACATACAT CGGTCTCACT AGAGACTTCA 
CTGGTAGTAG AGACCACTGT CACGCCAGGT ATCCCAACCA CTGCTATTGC ACCAAGCAAG 
CTCATGACAT CTACTACAAG TACATGCTTA CCACCAACCA GTTTGCAATT AGGAACATCT 
GGTTTGTCAG TTATACCAGT GGTCACGCCT GGGCAGGTTT CTACCCCAGT CAGCTATGTG 
TCAGCCCCAG GCAGCACTAC ATCAGGAGTG AAAGCTGGAA CTGCTCCATC AAAGCCACCT 
TTGACTAAAG CTCCAGTGCT GCCAGTGGGT ACTGAACTTC CAGCTGGTAC TCCACCCAGC 
GAGCAGCTGC CACCTTTTCC AGGACCTTCA CTAACTCAGT CCCAGCAACC TCTGGAAGAT 
CTTGATGCTC AGTTGAGAAG AACACTTAGT CCAGAGACTA TTGCGGTGAC ATCTACAATT 
GGTCCTGTGT CCATGGTGGC TCCAACAGCA GTCACAGAAG CAGGAGCACA GCCTCAGAAG 
GATGTTTCTC AGATCACAGA AGGTCCTGTC CTAGCGACTA GTTCGGGAAC TGGTGTTTTT 
AAGATGGGAA GATTTCAGGT TTCAGTTGCA ATGGATGATG TTCAAAAGGA GGGTAAAAAT 
AAGTTAGAAG ATGGAAAGTC TGTTCATTTT GAATCCAGCA CTTCAGAGTC CTCAGTGCTA 
TCAAGTAGTA GTCCAGAAAG TACCCTGGTG AAGACAGAGC CTAATGGGAT AACCATCCCT 
GGCATCTCAT CAGATATGCC AGATAGTGCC CACAAAACTC CTGCCTCAGA AGCAAAGTCA 
GAAACTGGGC AGCCTACCAA GGTTGGACGT TTTCAGGTGA CAACTACAGC AAACAAAGTA 
GGTCGTTTCT CTGTGTCAAG AACTGAAGAC AAGCTCATTG AGGCAAAGAA AGAGGGACCA 
ATAGCGTCTC CTCCTTTTAT GGATTTGGAA CAAGGTGTTC TTCCAGCTGT GATACCAAAG 
AAAGAAAAGC CTGAACTGTC AGAGCCTTCA CATCTGAACG GACCATCTTC TGACCTGGAG 
GCTGCCTTCC TGAGCAGGGA TGTGGATGAT GGTTCAGGTA GTCCACACTC CCCCCATCAG 
CTGAGCTCAA AGAGCCTGCC TATCCAGAAT CTAAGTCAAA GCCTTAGTAA TTCATTCAAC 
TCCTCTTACA TGAGTAGTGA CAATGAGTCA GATATTGAAG ATGAAGATTT AAAATTAGAG 
CTGCGAAGAC TACGAGAAAA ACATCTTAAA GAGATTCAAG ACCTGCAGAG TCGCCAGAAG 
CATGAAATTG AATCTTTGTA TACCAAACTG GGAAAGGTTC CCCCTGCTGT CATTATCCCC 
CCAGCTGCCC CCCTTTCAGG GAGAAGAAGG CGACCCACTA AAAGCAAAGG CAGCAAGTCT 
AGTCGCAGCA GTTCCTTGGG GAATAAAAGT CCCCAGCTTT CAGGTAACCT GTCTGGTCAG 
AGTGCTGCTT CAGTTTTGCA CGCTCAGCAG ACCCTCCACC CTCCTGGCAA CATCCCTGAG 
ACTGGGCCAA ATCAGCTGTT ACAGCCCCTT AAGCCATCTC CCTCCAGTGA CAACCTCTAC 
TCAGCCTTTA CCAGTGATGG TGCCATTTCA GTCCCAAGCC TTTCTGCTCC AGGTCAAGGC 
TGTGCGAAGT TTAACTGTGC ATCTGAGCAG GTGACATTCA AACCTGGTGG CAGGAGGACC 
CGATTTCTGA GTACGCCCTG CTTGGCTCTT TGGAAGATGG TGAAAAAAGT CTGTCCTTGC 
AACCAGCTCT GTATGTGCCC TCCAGCAGAG CCGAAATCAG GGACCAGCAG CACAAACACT 
GTCGGGGGAA CAGTGAACAG CCAAGCTGCC CCAGCTCAGC CTCCTGCCAT GACATCCAGC 
AGGAAGGGCA CATTCACAGA TGACCTACAC AAGTTGGTAG ACAATTGGGC CCGAGATGCC 
ATGAATCTCT CAGGCAGAAG AGGAAGCAAA GGACACATGA ATTATGAGGG CCCTGGAATG 
GCAAGAAAGT TCTCCGCACC TGGTCAGCTG TGCATCTCCA TGACCTCAAA TCTGGGTGGT 
TCTGCCCCCA TCTCTGCAGC ATCAGCTACC TCTCTAGGTC ACTTCACCAA GTCTATGTGC 
CCCCCACAGC AGTACGGTTT TCCAGCTCCC CCCTTTGGCA CTCAGTGGAG TGGGACGGGT 
GGCCCGGCAC CACAGCCACT TGGCCAGTTC CAGCCTGTGG GAACTGCCTC CTTACAGAAT 
TTCAACATCA GCAATTTGCA GAAATCCATC AGCAACCCCC CAGGTTCCAA CCTGCGGACC 
ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963804.1
CDS	99..7424
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVGQSQGQPSSSSLTGIPSSLPIQHSQQQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQLPVSQPVPTIQGEPQIPVAAQPSVVPVHSGAHFLPVGQPLSTSLLPQYPVSQIPISTPHASAPQTGFSSLPITMAAGINQPLLTLASSATAAAIPGGPTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPSRLPPQYPGDSNIAPSSSVASICIPSTVLSPPMPTEALATPGYFPTVVQPFVESNLLVPVGSIGGQVQVSQPAVSLAQQAPTTSSQQAALESTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGCAKFNCASEQVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCMCPPAEPKSGTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_014108329.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108329.1

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG 
CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT 
CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC 
CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA 
GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG 
GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT 
CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA 
CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT 
CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA 
GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA 
TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG 
CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG 
TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT 
CTGGAGAGCA CTCAGGGAGT CTCTCAAGTT GCTCCTCCAG AGCCAGTTCC AGTGGCACAG 
GCACAGCCTA CCACTTTGGT CTCCTCTATA GACAGTGCAC ATTCAGATGT TGCTTCAGGA 
ATGAGTGATG GCAATGAGAA TGTCCCGTCC TCCAGTGGAA GGCATGAAGG GAGAGCTACA 
AAACGACATT ACCGAAAATC TGTAAGAAGT CGCTCTCGTC ATGAAAAGAC TTCGCGTCCA 
AAATTAAGAA TTTTGAATGT TTCAAATAAA GGAGACCGAG TAGTAGAATG TCAACTAGAG 
ACTCATAATC GGAAAATGGT TACATTCAAA TTTGATTTAG ATGGTGACAA TCCTGAGGAG 
ATAGCAACAA TTATGGTGAA CAATGACTTT ATTCTAGCAA TGGAGAGAGA GTCCTTTGTG 
GATCAAGTAC GAGAAATTAT TGAAAAGGCT GATGAAATGC TCAGTGAGGA TGTCAGTGTG 
GAACCAGAGG GTGACCAGGG ATTGGAGAGT CTGCAGGGAA AGGATGACTA TGGCTTTACA 
GGTTCTCAAA AATTGGAAGG AGAATTCAAA CAACCAGTTC CTGCGTCTTC CATGCCACAG 
CAAATAGGCA TTCCTACCAG TTCTTTAACT CAAGTTGTTC ATTCTGCTGG AAGACGGTTT 
ATAGTGAGTC CTGTGCCAGA AAGTCGATTA CGAGAATCAA AAGTTTTCAC TAGTGAAATA 
CCAGATACAG TTGCTGCCTC TACTCACAGC GCTGGAATGA ACTTGTCTCA CTCTGCTTCA 
TCCCTTAGCC TACAGCAAGC CTTTTCTGAA CTTAGACATG TGCAGATGAC AGAAGGGCCC 
AGCACAGCAC CTCCCAACTT CAGTCATACA GGACCAACAT TTCCAGTTGT TCCTCCTTCC 
ATGAGTAGCA TTGCTGGAGT CCCAACCACA GCAGTAGCCA CACCTTCAGT ATCAGTCCCT 
GCAACTAGCA GCCCTCTAAA TGACAGTTCC ACATCAGTAA TTCAATCTGA GATTCCAGTG 
CCCACTGAAA CAGGGGTTGG TGGAGTTCCC ACCTGCACAA GTGTAATGCC TTCAAGTGGT 
CTCCCTGTAC CACCCATATC TGAATCGCCA ATATTTTCCA CTATGGTTTC AAGCATCACA 
GTACCTACAG TTGTCTCAAT ATCAGCAACA TCCCAGCCAG TTCAAGCTCC CACATCTGGG 
AGCACTGTTT CTAGCATAGG AACTTTGCCT TCTATACCAG TTTCAACAGC TTTAGCCTCT 
GCAGTGGGCA GTGTTGCTAT CCCAGGTGCT AAGCCTCCAC CTGTACTATC ACAGCAGGCA 
GCAGGCAGTA CTACTGGGGC AGCCACATTA ACATCCGTTC CTGCCATCAC TCCGTTCCCA 
AGCATAGCTT CACAGCCATC TCTTCAGCTT AGCAGCAGCA CCTCTGCTCC AACTCTAGCT 
GAAACAGTGG TGGTTAGTGC ACACTCACTA GATAAAACAT CTCATAGCAG TACAACTGGA 
TTGGCTTTGT CCCTGTCTGC ATCATCTTCC TCCCCTGGAG CAGGATTATC TAGTTCTATT 
TCTCAGCCTG GTGGGGTGCA TCCTTTGGTT ACCCCATCAG CTATGGCTTC TATTCCTGCC 
CTTCCTCAAA CAGCAGGACC TACTTCTACA CCTTTATTGC CCCAAGTACT TAGTATCCCA 
CCCTTGGTAC AACCTGTTGC CAGTGTGCCT GCTGTGCAGC AGACACTAAT TCATAGTCAG 
CCTCAACCAG CTTTACTTCC CAACCAGCCC CACACTCACT GTCCTGAAAT AGATGCTGAC 
TTACAGCCCA AAGCTCCTGG AATTGATGAC ATAAAGACTC TAGAGGAAAA GCTGCGGTCT 
CTCTTTAGTG AACCGAGCTC ATCTGGAGCC CAACATACAT CGGTCTCACT AGAGACTTCA 
CTGGTAGTAG AGACCACTGT CACGCCAGGT ATCCCAACCA CTGCTATTGC ACCAAGCAAG 
CTCATGACAT CTACTACAAG TACATGCTTA CCACCAACCA GTTTGCAATT AGGAACATCT 
GGTTTGTCAG TTATACCAGT GGTCACGCCT GGGCAGGTTT CTACCCCAGT CAGCTATGTG 
TCAGCCCCAG GCAGCACTAC ATCAGGAGTG AAAGCTGGAA CTGCTCCATC AAAGCCACCT 
TTGACTAAAG CTCCAGTGCT GCCAGTGGGT ACTGAACTTC CAGCTGGTAC TCCACCCAGC 
GAGCAGCTGC CACCTTTTCC AGGACCTTCA CTAACTCAGT CCCAGCAACC TCTGGAAGAT 
CTTGATGCTC AGTTGAGAAG AACACTTAGT CCAGAGACTA TTGCGGTGAC ATCTACAATT 
GGTCCTGTGT CCATGGTGGC TCCAACAGCA GTCACAGAAG CAGGAGCACA GCCTCAGAAG 
GATGTTTCTC AGATCACAGA AGGTCCTGTC CTAGCGACTA GTTCGGGAAC TGGTGTTTTT 
AAGATGGGAA GATTTCAGGT TTCAGTTGCA ATGGATGATG TTCAAAAGGA GGGTAAAAAT 
AAGTTAGAAG ATGGAAAGTC TGTTCATTTT GAATCCAGCA CTTCAGAGTC CTCAGTGCTA 
TCAAGTAGTA GTCCAGAAAG TACCCTGGTG AAGACAGAGC CTAATGGGAT AACCATCCCT 
GGCATCTCAT CAGATATGCC AGATAGTGCC CACAAAACTC CTGCCTCAGA AGCAAAGTCA 
GAAACTGGGC AGCCTACCAA GGTTGGACGT TTTCAGGTGA CAACTACAGC AAACAAAGTA 
GGTCGTTTCT CTGTGTCAAG AACTGAAGAC AAGCTCATTG AGGCAAAGAA AGAGGGACCA 
ATAGCGTCTC CTCCTTTTAT GGATTTGGAA CAAGGTGTTC TTCCAGCTGT GATACCAAAG 
AAAGAAAAGC CTGAACTGTC AGAGCCTTCA CATCTGAACG GACCATCTTC TGACCTGGAG 
GCTGCCTTCC TGAGCAGGGA TGTGGATGAT GGTTCAGGTA GTCCACACTC CCCCCATCAG 
CTGAGCTCAA AGAGCCTGCC TATCCAGAAT CTAAGTCAAA GCCTTAGTAA TTCATTCAAC 
TCCTCTTACA TGAGTAGTGA CAATGAGTCA GATATTGAAG ATGAAGATTT AAAATTAGAG 
CTGCGAAGAC TACGAGAAAA ACATCTTAAA GAGATTCAAG ACCTGCAGAG TCGCCAGAAG 
CATGAAATTG AATCTTTGTA TACCAAACTG GGAAAGGTTC CCCCTGCTGT CATTATCCCC 
CCAGCTGCCC CCCTTTCAGG GAGAAGAAGG CGACCCACTA AAAGCAAAGG CAGCAAGTCT 
AGTCGCAGCA GTTCCTTGGG GAATAAAAGT CCCCAGCTTT CAGGTAACCT GTCTGGTCAG 
AGTGCTGCTT CAGTTTTGCA CGCTCAGCAG ACCCTCCACC CTCCTGGCAA CATCCCTGAG 
ACTGGGCCAA ATCAGCTGTT ACAGCCCCTT AAGCCATCTC CCTCCAGTGA CAACCTCTAC 
TCAGCCTTTA CCAGTGATGG TGCCATTTCA GTCCCAAGCC TTTCTGCTCC AGGTCAAGGC 
TGTGCGAAGT TTAACTGTGC ATCTGAGCAG GTGACATTCA AACCTGGTGG CAGGAGGACC 
CGATTTCTGA GTACGCCCTG CTTGGCTCTT TGGAAGATGG TGAAAAAAGT CTGTCCTTGC 
AACCAGCTCT GTATGTGCCC TCCAGCAGAG CCGAAATCAG GGACCAGCAG CACAAACACT 
GTCGGGGGAA CAGTGAACAG CCAAGCTGCC CCAGCTCAGC CTCCTGCCAT GACATCCAGC 
AGGAAGGGCA CATTCACAGA TGACCTACAC AAGTTGGTAG ACAATTGGGC CCGAGATGCC 
ATGAATCTCT CAGGCAGAAG AGGAAGCAAA GGACACATGA ATTATGAGGG CCCTGGAATG 
GCAAGAAAGT TCTCCGCACC TGGTCAGCTG TGCATCTCCA TGACCTCAAA TCTGGGTGGT 
TCTGCCCCCA TCTCTGCAGC ATCAGCTACC TCTCTAGGTC ACTTCACCAA GTCTATGTGC 
CCCCCACAGC AGTACGGTTT TCCAGCTCCC CCCTTTGGCA CTCAGTGGAG TGGGACGGGT 
GGCCCGGCAC CACAGCCACT TGGCCAGTTC CAGCCTGTGG GAACTGCCTC CTTACAGAAT 
TTCAACATCA GCAATTTGCA GAAATCCATC AGCAACCCCC CAGGTTCCAA CCTGCGGACC 
ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_014108330.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963805.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X7, mRNA.

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CloneID	OCe43575
Clone ID Related Accession (Same CDS sequence)	XM_014108330.1
Accession Version	XM_014108330.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7302bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase WNK1 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGCAG 
CAGGGAGTAC AGCAGACAGC CCCTCCACAA CAGACAGCGC AGTATTCGCT TCCACAGACA 
TCAGCCTCCA GTGAGGCCAC TATTGCACAA CCAGTCAGTC AGTCTCAGGC TCCACAGGTC 
TTGCCTCAAA TATCAGCTGG AAAACAGCTT CCAGTTTCCC AGCCAGTACC AACTATCCAA 
GGCGAACCTC AGATCCCAGT TGCGGCACAA CCCTCAGTTG TTCCAGTCCA CTCTGGTGCT 
CATTTCCTCC CTGTGGGACA GCCACTCTCT ACTTCCTTAC TCCCTCAGTA CCCGGTCTCT 
CAAATTCCCA TATCAACTCC TCATGCGTCT GCGCCCCAGA CAGGTTTTTC ATCCCTTCCC 
ATTACAATGG CAGCTGGCAT TAATCAGCCT CTGCTCACCT TGGCTTCATC TGCTACAGCA 
GCTGCGATCC CAGGGGGACC AACTGTGGTT CCTAGTCAGC TTCCAACCCT TCTGCAGCCT 
GTGACTCAGC TGCCAAGTCA GGTTCACCCA CAACTCCTGC AACCAGCAGT TCAGTCCATG 
GGAATACCAG CTAACCTTGG ACAAGCTGCT GAGGTTCCAC TTCCCTCTGG AGATGTTCTG 
TACCAGGGCT TTCCATCTCG ACTGCCACCA CAGTACCCAG GAGATTCAAA TATTGCTCCT 
TCTTCCAGCG TGGCTTCTAT TTGCATCCCT TCTACAGTCC TATCCCCTCC CATGCCCACA 
GAAGCACTGG CTACACCGGG TTACTTTCCT ACAGTGGTGC AGCCTTTTGT GGAATCTAAC 
CTTTTGGTTC CTGTGGGCAG TATAGGAGGA CAGGTTCAAG TGTCCCAGCC AGCAGTGAGT 
TTAGCACAAC AAGCCCCCAC TACATCCTCC CAGCAAGCAG CTCTGGAGCA GAATGAAATG 
CCAATGTGGG GGCCTTGTTG CCACTGGCTG TTCTGTTGGG AAAGCACTCA GGGAGTCTCT 
CAAGTTGCTC CTCCAGAGCC AGTTCCAGTG GCACAGGCAC AGCCTACCAC TTTGGTCTCC 
TCTATAGACA GTGCACATTC AGATGTTGCT TCAGGAATGA GTGATGGCAA TGAGAATGTC 
CCGTCCTCCA GTGGAAGGCA TGAAGGGAGA GCTACAAAAC GACATTACCG AAAATCTGTA 
AGAAGTCGCT CTCGTCATGA AAAGACTTCG CGTCCAAAAT TAAGAATTTT GAATGTTTCA 
AATAAAGGAG ACCGAGTAGT AGAATGTCAA CTAGAGACTC ATAATCGGAA AATGGTTACA 
TTCAAATTTG ATTTAGATGG TGACAATCCT GAGGAGATAG CAACAATTAT GGTGAACAAT 
GACTTTATTC TAGCAATGGA GAGAGAGTCC TTTGTGGATC AAGTACGAGA AATTATTGAA 
AAGGCTGATG AAATGCTCAG TGAGGATGTC AGTGTGGAAC CAGAGGGTGA CCAGGGATTG 
GAGAGTCTGC AGGGAAAGGA TGACTATGGC TTTACAGGTT CTCAAAAATT GGAAGGAGAA 
TTCAAACAAC CAGTTCCTGC GTCTTCCATG CCACAGCAAA TAGGCATTCC TACCAGTTCT 
TTAACTCAAG TTGTTCATTC TGCTGGAAGA CGGTTTATAG TGAGTCCTGT GCCAGAAAGT 
CGATTACGAG AATCAAAAGT TTTCACTAGT GAAATACCAG ATACAGTTGC TGCCTCTACT 
CACAGCGCTG GAATGAACTT GTCTCACTCT GCTTCATCCC TTAGCCTACA GCAAGCCTTT 
TCTGAACTTA GACATGTGCA GATGACAGAA GGGCCCAGCA CAGCACCTCC CAACTTCAGT 
CATACAGGAC CAACATTTCC AGTTGTTCCT CCTTCCATGA GTAGCATTGC TGGAGTCCCA 
ACCACAGCAG TAGCCACACC TTCAGTATCA GTCCCTGCAA CTAGCAGCCC TCTAAATGAC 
AGTTCCACAT CAGTAATTCA ATCTGAGATT CCAGTGCCCA CTGAAACAGG GGTTGGTGGA 
GTTCCCACCT GCACAAGTGT AATGCCTTCA AGTGGTCTCC CTGTACCACC CATATCTGAA 
TCGCCAATAT TTTCCACTAT GGTTTCAAGC ATCACAGTAC CTACAGTTGT CTCAATATCA 
GCAACATCCC AGCCAGTTCA AGCTCCCACA TCTGGGAGCA CTGTTTCTAG CATAGGAACT 
TTGCCTTCTA TACCAGTTTC AACAGCTTTA GCCTCTGCAG TGGGCAGTGT TGCTATCCCA 
GGTGCTAAGC CTCCACCTGT ACTATCACAG CAGGCAGCAG GCAGTACTAC TGGGGCAGCC 
ACATTAACAT CCGTTCCTGC CATCACTCCG TTCCCAAGCA TAGCTTCACA GCCATCTCTT 
CAGCTTAGCA GCAGCACCTC TGCTCCAACT CTAGCTGAAA CAGTGGTGGT TAGTGCACAC 
TCACTAGATA AAACATCTCA TAGCAGTACA ACTGGATTGG CTTTGTCCCT GTCTGCATCA 
TCTTCCTCCC CTGGAGCAGG ATTATCTAGT TCTATTTCTC AGCCTGGTGG GGTGCATCCT 
TTGGTTACCC CATCAGCTAT GGCTTCTATT CCTGCCCTTC CTCAAACAGC AGGACCTACT 
TCTACACCTT TATTGCCCCA AGTACTTAGT ATCCCACCCT TGGTACAACC TGTTGCCAGT 
GTGCCTGCTG TGCAGCAGAC ACTAATTCAT AGTCAGCCTC AACCAGCTTT ACTTCCCAAC 
CAGCCCCACA CTCACTGTCC TGAAATAGAT GCTGACTTAC AGCCCAAAGC TCCTGGAATT 
GATGACATAA AGACTCTAGA GGAAAAGCTG CGGTCTCTCT TTAGTGAACC GAGCTCATCT 
GGAGCCCAAC ATACATCGGT CTCACTAGAG ACTTCACTGG TAGTAGAGAC CACTGTCACG 
CCAGGTATCC CAACCACTGC TATTGCACCA AGCAAGCTCA TGACATCTAC TACAAGTACA 
TGCTTACCAC CAACCAGTTT GCAATTAGGA ACATCTGGTT TGTCAGTTAT ACCAGTGGTC 
ACGCCTGGGC AGGTTTCTAC CCCAGTCAGC TATGTGTCAG CCCCAGGCAG CACTACATCA 
GGAGTGAAAG CTGGAACTGC TCCATCAAAG CCACCTTTGA CTAAAGCTCC AGTGCTGCCA 
GTGGGTACTG AACTTCCAGC TGGTACTCCA CCCAGCGAGC AGCTGCCACC TTTTCCAGGA 
CCTTCACTAA CTCAGTCCCA GCAACCTCTG GAAGATCTTG ATGCTCAGTT GAGAAGAACA 
CTTAGTCCAG AGACTATTGC GGTGACATCT ACAATTGGTC CTGTGTCCAT GGTGGCTCCA 
ACAGCAGTCA CAGAAGCAGG AGCACAGCCT CAGAAGGATG TTTCTCAGAT CACAGAAGGT 
CCTGTCCTAG CGACTAGTTC GGGAACTGGT GTTTTTAAGA TGGGAAGATT TCAGGTTTCA 
GTTGCAATGG ATGATGTTCA AAAGGAGGGT AAAAATAAGT TAGAAGATGG AAAGTCTGTT 
CATTTTGAAT CCAGCACTTC AGAGTCCTCA GTGCTATCAA GTAGTAGTCC AGAAAGTACC 
CTGGTGAAGA CAGAGCCTAA TGGGATAACC ATCCCTGGCA TCTCATCAGA TATGCCAGAT 
AGTGCCCACA AAACTCCTGC CTCAGAAGCA AAGTCAGAAA CTGGGCAGCC TACCAAGGTT 
GGACGTTTTC AGGTGACAAC TACAGCAAAC AAAGTAGGTC GTTTCTCTGT GTCAAGAACT 
GAAGACAAGC TCATTGAGGC AAAGAAAGAG GGACCAATAG CGTCTCCTCC TTTTATGGAT 
TTGGAACAAG GTGTTCTTCC AGCTGTGATA CCAAAGAAAG AAAAGCCTGA ACTGTCAGAG 
CCTTCACATC TGAACGGACC ATCTTCTGAC CTGGAGGCTG CCTTCCTGAG CAGGGATGTG 
GATGATGGTT CAGGTAGTCC ACACTCCCCC CATCAGCTGA GCTCAAAGAG CCTGCCTATC 
CAGAATCTAA GTCAAAGCCT TAGTAATTCA TTCAACTCCT CTTACATGAG TAGTGACAAT 
GAGTCAGATA TTGAAGATGA AGATTTAAAA TTAGAGCTGC GAAGACTACG AGAAAAACAT 
CTTAAAGAGA TTCAAGACCT GCAGAGTCGC CAGAAGCATG AAATTGAATC TTTGTATACC 
AAACTGGGAA AGGTTCCCCC TGCTGTCATT ATCCCCCCAG CTGCCCCCCT TTCAGGGAGA 
AGAAGGCGAC CCACTAAAAG CAAAGGCAGC AAGTCTAGTC GCAGCAGTTC CTTGGGGAAT 
AAAAGTCCCC AGCTTTCAGG TAACCTGTCT GGTCAGAGTG CTGCTTCAGT TTTGCACGCT 
CAGCAGACCC TCCACCCTCC TGGCAACATC CCTGAGACTG GGCCAAATCA GCTGTTACAG 
CCCCTTAAGC CATCTCCCTC CAGTGACAAC CTCTACTCAG CCTTTACCAG TGATGGTGCC 
ATTTCAGTCC CAAGCCTTTC TGCTCCAGGT CAAGGCTGTG CGAAGTTTAA CTGTGCATCT 
GAGCAGGTGA CATTCAAACC TGGTGGCAGG AGGACCCGAT TTCTGAGTAC GCCCTGCTTG 
GCTCTTTGGA AGATGGTGAA AAAAGTCTGT CCTTGCAACC AGCTCTGTAT GTGCCCTCCA 
GCAGAGCCGA AATCAGGGAC CAGCAGCACA AACACTGTCG GGGGAACAGT GAACAGCCAA 
GCTGCCCCAG CTCAGCCTCC TGCCATGACA TCCAGCAGGA AGGGCACATT CACAGATGAC 
CTACACAAGT TGGTAGACAA TTGGGCCCGA GATGCCATGA ATCTCTCAGG CAGAAGAGGA 
AGCAAAGGAC ACATGAATTA TGAGGGCCCT GGAATGGCAA GAAAGTTCTC CGCACCTGGT 
CAGCTGTGCA TCTCCATGAC CTCAAATCTG GGTGGTTCTG CCCCCATCTC TGCAGCATCA 
GCTACCTCTC TAGGTCACTT CACCAAGTCT ATGTGCCCCC CACAGCAGTA CGGTTTTCCA 
GCTCCCCCCT TTGGCACTCA GTGGAGTGGG ACGGGTGGCC CGGCACCACA GCCACTTGGC 
CAGTTCCAGC CTGTGGGAAC TGCCTCCTTA CAGAATTTCA ACATCAGCAA TTTGCAGAAA 
TCCATCAGCA ACCCCCCAGG TTCCAACCTG CGGACCACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963805.1
CDS	99..7400
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQLPVSQPVPTIQGEPQIPVAAQPSVVPVHSGAHFLPVGQPLSTSLLPQYPVSQIPISTPHASAPQTGFSSLPITMAAGINQPLLTLASSATAAAIPGGPTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPSRLPPQYPGDSNIAPSSSVASICIPSTVLSPPMPTEALATPGYFPTVVQPFVESNLLVPVGSIGGQVQVSQPAVSLAQQAPTTSSQQAALEQNEMPMWGPCCHWLFCWESTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGCAKFNCASEQVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCMCPPAEPKSGTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_014108330.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108330.1

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGCAG 
CAGGGAGTAC AGCAGACAGC CCCTCCACAA CAGACAGCGC AGTATTCGCT TCCACAGACA 
TCAGCCTCCA GTGAGGCCAC TATTGCACAA CCAGTCAGTC AGTCTCAGGC TCCACAGGTC 
TTGCCTCAAA TATCAGCTGG AAAACAGCTT CCAGTTTCCC AGCCAGTACC AACTATCCAA 
GGCGAACCTC AGATCCCAGT TGCGGCACAA CCCTCAGTTG TTCCAGTCCA CTCTGGTGCT 
CATTTCCTCC CTGTGGGACA GCCACTCTCT ACTTCCTTAC TCCCTCAGTA CCCGGTCTCT 
CAAATTCCCA TATCAACTCC TCATGCGTCT GCGCCCCAGA CAGGTTTTTC ATCCCTTCCC 
ATTACAATGG CAGCTGGCAT TAATCAGCCT CTGCTCACCT TGGCTTCATC TGCTACAGCA 
GCTGCGATCC CAGGGGGACC AACTGTGGTT CCTAGTCAGC TTCCAACCCT TCTGCAGCCT 
GTGACTCAGC TGCCAAGTCA GGTTCACCCA CAACTCCTGC AACCAGCAGT TCAGTCCATG 
GGAATACCAG CTAACCTTGG ACAAGCTGCT GAGGTTCCAC TTCCCTCTGG AGATGTTCTG 
TACCAGGGCT TTCCATCTCG ACTGCCACCA CAGTACCCAG GAGATTCAAA TATTGCTCCT 
TCTTCCAGCG TGGCTTCTAT TTGCATCCCT TCTACAGTCC TATCCCCTCC CATGCCCACA 
GAAGCACTGG CTACACCGGG TTACTTTCCT ACAGTGGTGC AGCCTTTTGT GGAATCTAAC 
CTTTTGGTTC CTGTGGGCAG TATAGGAGGA CAGGTTCAAG TGTCCCAGCC AGCAGTGAGT 
TTAGCACAAC AAGCCCCCAC TACATCCTCC CAGCAAGCAG CTCTGGAGCA GAATGAAATG 
CCAATGTGGG GGCCTTGTTG CCACTGGCTG TTCTGTTGGG AAAGCACTCA GGGAGTCTCT 
CAAGTTGCTC CTCCAGAGCC AGTTCCAGTG GCACAGGCAC AGCCTACCAC TTTGGTCTCC 
TCTATAGACA GTGCACATTC AGATGTTGCT TCAGGAATGA GTGATGGCAA TGAGAATGTC 
CCGTCCTCCA GTGGAAGGCA TGAAGGGAGA GCTACAAAAC GACATTACCG AAAATCTGTA 
AGAAGTCGCT CTCGTCATGA AAAGACTTCG CGTCCAAAAT TAAGAATTTT GAATGTTTCA 
AATAAAGGAG ACCGAGTAGT AGAATGTCAA CTAGAGACTC ATAATCGGAA AATGGTTACA 
TTCAAATTTG ATTTAGATGG TGACAATCCT GAGGAGATAG CAACAATTAT GGTGAACAAT 
GACTTTATTC TAGCAATGGA GAGAGAGTCC TTTGTGGATC AAGTACGAGA AATTATTGAA 
AAGGCTGATG AAATGCTCAG TGAGGATGTC AGTGTGGAAC CAGAGGGTGA CCAGGGATTG 
GAGAGTCTGC AGGGAAAGGA TGACTATGGC TTTACAGGTT CTCAAAAATT GGAAGGAGAA 
TTCAAACAAC CAGTTCCTGC GTCTTCCATG CCACAGCAAA TAGGCATTCC TACCAGTTCT 
TTAACTCAAG TTGTTCATTC TGCTGGAAGA CGGTTTATAG TGAGTCCTGT GCCAGAAAGT 
CGATTACGAG AATCAAAAGT TTTCACTAGT GAAATACCAG ATACAGTTGC TGCCTCTACT 
CACAGCGCTG GAATGAACTT GTCTCACTCT GCTTCATCCC TTAGCCTACA GCAAGCCTTT 
TCTGAACTTA GACATGTGCA GATGACAGAA GGGCCCAGCA CAGCACCTCC CAACTTCAGT 
CATACAGGAC CAACATTTCC AGTTGTTCCT CCTTCCATGA GTAGCATTGC TGGAGTCCCA 
ACCACAGCAG TAGCCACACC TTCAGTATCA GTCCCTGCAA CTAGCAGCCC TCTAAATGAC 
AGTTCCACAT CAGTAATTCA ATCTGAGATT CCAGTGCCCA CTGAAACAGG GGTTGGTGGA 
GTTCCCACCT GCACAAGTGT AATGCCTTCA AGTGGTCTCC CTGTACCACC CATATCTGAA 
TCGCCAATAT TTTCCACTAT GGTTTCAAGC ATCACAGTAC CTACAGTTGT CTCAATATCA 
GCAACATCCC AGCCAGTTCA AGCTCCCACA TCTGGGAGCA CTGTTTCTAG CATAGGAACT 
TTGCCTTCTA TACCAGTTTC AACAGCTTTA GCCTCTGCAG TGGGCAGTGT TGCTATCCCA 
GGTGCTAAGC CTCCACCTGT ACTATCACAG CAGGCAGCAG GCAGTACTAC TGGGGCAGCC 
ACATTAACAT CCGTTCCTGC CATCACTCCG TTCCCAAGCA TAGCTTCACA GCCATCTCTT 
CAGCTTAGCA GCAGCACCTC TGCTCCAACT CTAGCTGAAA CAGTGGTGGT TAGTGCACAC 
TCACTAGATA AAACATCTCA TAGCAGTACA ACTGGATTGG CTTTGTCCCT GTCTGCATCA 
TCTTCCTCCC CTGGAGCAGG ATTATCTAGT TCTATTTCTC AGCCTGGTGG GGTGCATCCT 
TTGGTTACCC CATCAGCTAT GGCTTCTATT CCTGCCCTTC CTCAAACAGC AGGACCTACT 
TCTACACCTT TATTGCCCCA AGTACTTAGT ATCCCACCCT TGGTACAACC TGTTGCCAGT 
GTGCCTGCTG TGCAGCAGAC ACTAATTCAT AGTCAGCCTC AACCAGCTTT ACTTCCCAAC 
CAGCCCCACA CTCACTGTCC TGAAATAGAT GCTGACTTAC AGCCCAAAGC TCCTGGAATT 
GATGACATAA AGACTCTAGA GGAAAAGCTG CGGTCTCTCT TTAGTGAACC GAGCTCATCT 
GGAGCCCAAC ATACATCGGT CTCACTAGAG ACTTCACTGG TAGTAGAGAC CACTGTCACG 
CCAGGTATCC CAACCACTGC TATTGCACCA AGCAAGCTCA TGACATCTAC TACAAGTACA 
TGCTTACCAC CAACCAGTTT GCAATTAGGA ACATCTGGTT TGTCAGTTAT ACCAGTGGTC 
ACGCCTGGGC AGGTTTCTAC CCCAGTCAGC TATGTGTCAG CCCCAGGCAG CACTACATCA 
GGAGTGAAAG CTGGAACTGC TCCATCAAAG CCACCTTTGA CTAAAGCTCC AGTGCTGCCA 
GTGGGTACTG AACTTCCAGC TGGTACTCCA CCCAGCGAGC AGCTGCCACC TTTTCCAGGA 
CCTTCACTAA CTCAGTCCCA GCAACCTCTG GAAGATCTTG ATGCTCAGTT GAGAAGAACA 
CTTAGTCCAG AGACTATTGC GGTGACATCT ACAATTGGTC CTGTGTCCAT GGTGGCTCCA 
ACAGCAGTCA CAGAAGCAGG AGCACAGCCT CAGAAGGATG TTTCTCAGAT CACAGAAGGT 
CCTGTCCTAG CGACTAGTTC GGGAACTGGT GTTTTTAAGA TGGGAAGATT TCAGGTTTCA 
GTTGCAATGG ATGATGTTCA AAAGGAGGGT AAAAATAAGT TAGAAGATGG AAAGTCTGTT 
CATTTTGAAT CCAGCACTTC AGAGTCCTCA GTGCTATCAA GTAGTAGTCC AGAAAGTACC 
CTGGTGAAGA CAGAGCCTAA TGGGATAACC ATCCCTGGCA TCTCATCAGA TATGCCAGAT 
AGTGCCCACA AAACTCCTGC CTCAGAAGCA AAGTCAGAAA CTGGGCAGCC TACCAAGGTT 
GGACGTTTTC AGGTGACAAC TACAGCAAAC AAAGTAGGTC GTTTCTCTGT GTCAAGAACT 
GAAGACAAGC TCATTGAGGC AAAGAAAGAG GGACCAATAG CGTCTCCTCC TTTTATGGAT 
TTGGAACAAG GTGTTCTTCC AGCTGTGATA CCAAAGAAAG AAAAGCCTGA ACTGTCAGAG 
CCTTCACATC TGAACGGACC ATCTTCTGAC CTGGAGGCTG CCTTCCTGAG CAGGGATGTG 
GATGATGGTT CAGGTAGTCC ACACTCCCCC CATCAGCTGA GCTCAAAGAG CCTGCCTATC 
CAGAATCTAA GTCAAAGCCT TAGTAATTCA TTCAACTCCT CTTACATGAG TAGTGACAAT 
GAGTCAGATA TTGAAGATGA AGATTTAAAA TTAGAGCTGC GAAGACTACG AGAAAAACAT 
CTTAAAGAGA TTCAAGACCT GCAGAGTCGC CAGAAGCATG AAATTGAATC TTTGTATACC 
AAACTGGGAA AGGTTCCCCC TGCTGTCATT ATCCCCCCAG CTGCCCCCCT TTCAGGGAGA 
AGAAGGCGAC CCACTAAAAG CAAAGGCAGC AAGTCTAGTC GCAGCAGTTC CTTGGGGAAT 
AAAAGTCCCC AGCTTTCAGG TAACCTGTCT GGTCAGAGTG CTGCTTCAGT TTTGCACGCT 
CAGCAGACCC TCCACCCTCC TGGCAACATC CCTGAGACTG GGCCAAATCA GCTGTTACAG 
CCCCTTAAGC CATCTCCCTC CAGTGACAAC CTCTACTCAG CCTTTACCAG TGATGGTGCC 
ATTTCAGTCC CAAGCCTTTC TGCTCCAGGT CAAGGCTGTG CGAAGTTTAA CTGTGCATCT 
GAGCAGGTGA CATTCAAACC TGGTGGCAGG AGGACCCGAT TTCTGAGTAC GCCCTGCTTG 
GCTCTTTGGA AGATGGTGAA AAAAGTCTGT CCTTGCAACC AGCTCTGTAT GTGCCCTCCA 
GCAGAGCCGA AATCAGGGAC CAGCAGCACA AACACTGTCG GGGGAACAGT GAACAGCCAA 
GCTGCCCCAG CTCAGCCTCC TGCCATGACA TCCAGCAGGA AGGGCACATT CACAGATGAC 
CTACACAAGT TGGTAGACAA TTGGGCCCGA GATGCCATGA ATCTCTCAGG CAGAAGAGGA 
AGCAAAGGAC ACATGAATTA TGAGGGCCCT GGAATGGCAA GAAAGTTCTC CGCACCTGGT 
CAGCTGTGCA TCTCCATGAC CTCAAATCTG GGTGGTTCTG CCCCCATCTC TGCAGCATCA 
GCTACCTCTC TAGGTCACTT CACCAAGTCT ATGTGCCCCC CACAGCAGTA CGGTTTTCCA 
GCTCCCCCCT TTGGCACTCA GTGGAGTGGG ACGGGTGGCC CGGCACCACA GCCACTTGGC 
CAGTTCCAGC CTGTGGGAAC TGCCTCCTTA CAGAATTTCA ACATCAGCAA TTTGCAGAAA 
TCCATCAGCA ACCCCCCAGG TTCCAACCTG CGGACCACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_014108331.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963806.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X8, mRNA.

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CloneID	OCe43576
Clone ID Related Accession (Same CDS sequence)	XM_014108331.1
Accession Version	XM_014108331.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase WNK1 isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGCAG 
GGAGTACAGC AGACAGCCCC TCCACAACAG ACAGCGCAGT ATTCGCTTCC ACAGACATCA 
GCCTCCAGTG AGGCCACTAT TGCACAACCA GTCAGTCAGT CTCAGGCTCC ACAGGTCTTG 
CCTCAAATAT CAGCTGGAAA ACAGCTTCCA GTTTCCCAGC CAGTACCAAC TATCCAAGGC 
GAACCTCAGA TCCCAGTTGC GGCACAACCC TCAGTTGTTC CAGTCCACTC TGGTGCTCAT 
TTCCTCCCTG TGGGACAGCC ACTCTCTACT TCCTTACTCC CTCAGTACCC GGTCTCTCAA 
ATTCCCATAT CAACTCCTCA TGCGTCTGCG CCCCAGACAG GTTTTTCATC CCTTCCCATT 
ACAATGGCAG CTGGCATTAA TCAGCCTCTG CTCACCTTGG CTTCATCTGC TACAGCAGCT 
GCGATCCCAG GGGGACCAAC TGTGGTTCCT AGTCAGCTTC CAACCCTTCT GCAGCCTGTG 
ACTCAGCTGC CAAGTCAGGT TCACCCACAA CTCCTGCAAC CAGCAGTTCA GTCCATGGGA 
ATACCAGCTA ACCTTGGACA AGCTGCTGAG GTTCCACTTC CCTCTGGAGA TGTTCTGTAC 
CAGGGCTTTC CATCTCGACT GCCACCACAG TACCCAGGAG ATTCAAATAT TGCTCCTTCT 
TCCAGCGTGG CTTCTATTTG CATCCCTTCT ACAGTCCTAT CCCCTCCCAT GCCCACAGAA 
GCACTGGCTA CACCGGGTTA CTTTCCTACA GTGGTGCAGC CTTTTGTGGA ATCTAACCTT 
TTGGTTCCTG TGGGCAGTAT AGGAGGACAG GTTCAAGTGT CCCAGCCAGC AGTGAGTTTA 
GCACAACAAG CCCCCACTAC ATCCTCCCAG CAAGCAGCTC TGGAGCAGAA TGAAATGCCA 
ATGTGGGGGC CTTGTTGCCA CTGGCTGTTC TGTTGGGAAA GCACTCAGGG AGTCTCTCAA 
GTTGCTCCTC CAGAGCCAGT TCCAGTGGCA CAGGCACAGC CTACCACTTT GGTCTCCTCT 
ATAGACAGTG CACATTCAGA TGTTGCTTCA GGAATGAGTG ATGGCAATGA GAATGTCCCG 
TCCTCCAGTG GAAGGCATGA AGGGAGAGCT ACAAAACGAC ATTACCGAAA ATCTGTAAGA 
AGTCGCTCTC GTCATGAAAA GACTTCGCGT CCAAAATTAA GAATTTTGAA TGTTTCAAAT 
AAAGGAGACC GAGTAGTAGA ATGTCAACTA GAGACTCATA ATCGGAAAAT GGTTACATTC 
AAATTTGATT TAGATGGTGA CAATCCTGAG GAGATAGCAA CAATTATGGT GAACAATGAC 
TTTATTCTAG CAATGGAGAG AGAGTCCTTT GTGGATCAAG TACGAGAAAT TATTGAAAAG 
GCTGATGAAA TGCTCAGTGA GGATGTCAGT GTGGAACCAG AGGGTGACCA GGGATTGGAG 
AGTCTGCAGG GAAAGGATGA CTATGGCTTT ACAGGTTCTC AAAAATTGGA AGGAGAATTC 
AAACAACCAG TTCCTGCGTC TTCCATGCCA CAGCAAATAG GCATTCCTAC CAGTTCTTTA 
ACTCAAGTTG TTCATTCTGC TGGAAGACGG TTTATAGTGA GTCCTGTGCC AGAAAGTCGA 
TTACGAGAAT CAAAAGTTTT CACTAGTGAA ATACCAGATA CAGTTGCTGC CTCTACTCAC 
AGCGCTGGAA TGAACTTGTC TCACTCTGCT TCATCCCTTA GCCTACAGCA AGCCTTTTCT 
GAACTTAGAC ATGTGCAGAT GACAGAAGGG CCCAGCACAG CACCTCCCAA CTTCAGTCAT 
ACAGGACCAA CATTTCCAGT TGTTCCTCCT TCCATGAGTA GCATTGCTGG AGTCCCAACC 
ACAGCAGTAG CCACACCTTC AGTATCAGTC CCTGCAACTA GCAGCCCTCT AAATGACAGT 
TCCACATCAG TAATTCAATC TGAGATTCCA GTGCCCACTG AAACAGGGGT TGGTGGAGTT 
CCCACCTGCA CAAGTGTAAT GCCTTCAAGT GGTCTCCCTG TACCACCCAT ATCTGAATCG 
CCAATATTTT CCACTATGGT TTCAAGCATC ACAGTACCTA CAGTTGTCTC AATATCAGCA 
ACATCCCAGC CAGTTCAAGC TCCCACATCT GGGAGCACTG TTTCTAGCAT AGGAACTTTG 
CCTTCTATAC CAGTTTCAAC AGCTTTAGCC TCTGCAGTGG GCAGTGTTGC TATCCCAGGT 
GCTAAGCCTC CACCTGTACT ATCACAGCAG GCAGCAGGCA GTACTACTGG GGCAGCCACA 
TTAACATCCG TTCCTGCCAT CACTCCGTTC CCAAGCATAG CTTCACAGCC ATCTCTTCAG 
CTTAGCAGCA GCACCTCTGC TCCAACTCTA GCTGAAACAG TGGTGGTTAG TGCACACTCA 
CTAGATAAAA CATCTCATAG CAGTACAACT GGATTGGCTT TGTCCCTGTC TGCATCATCT 
TCCTCCCCTG GAGCAGGATT ATCTAGTTCT ATTTCTCAGC CTGGTGGGGT GCATCCTTTG 
GTTACCCCAT CAGCTATGGC TTCTATTCCT GCCCTTCCTC AAACAGCAGG ACCTACTTCT 
ACACCTTTAT TGCCCCAAGT ACTTAGTATC CCACCCTTGG TACAACCTGT TGCCAGTGTG 
CCTGCTGTGC AGCAGACACT AATTCATAGT CAGCCTCAAC CAGCTTTACT TCCCAACCAG 
CCCCACACTC ACTGTCCTGA AATAGATGCT GACTTACAGC CCAAAGCTCC TGGAATTGAT 
GACATAAAGA CTCTAGAGGA AAAGCTGCGG TCTCTCTTTA GTGAACCGAG CTCATCTGGA 
GCCCAACATA CATCGGTCTC ACTAGAGACT TCACTGGTAG TAGAGACCAC TGTCACGCCA 
GGTATCCCAA CCACTGCTAT TGCACCAAGC AAGCTCATGA CATCTACTAC AAGTACATGC 
TTACCACCAA CCAGTTTGCA ATTAGGAACA TCTGGTTTGT CAGTTATACC AGTGGTCACG 
CCTGGGCAGG TTTCTACCCC AGTCAGCTAT GTGTCAGCCC CAGGCAGCAC TACATCAGGA 
GTGAAAGCTG GAACTGCTCC ATCAAAGCCA CCTTTGACTA AAGCTCCAGT GCTGCCAGTG 
GGTACTGAAC TTCCAGCTGG TACTCCACCC AGCGAGCAGC TGCCACCTTT TCCAGGACCT 
TCACTAACTC AGTCCCAGCA ACCTCTGGAA GATCTTGATG CTCAGTTGAG AAGAACACTT 
AGTCCAGAGA CTATTGCGGT GACATCTACA ATTGGTCCTG TGTCCATGGT GGCTCCAACA 
GCAGTCACAG AAGCAGGAGC ACAGCCTCAG AAGGATGTTT CTCAGATCAC AGAAGGTCCT 
GTCCTAGCGA CTAGTTCGGG AACTGGTGTT TTTAAGATGG GAAGATTTCA GGTTTCAGTT 
GCAATGGATG ATGTTCAAAA GGAGGGTAAA AATAAGTTAG AAGATGGAAA GTCTGTTCAT 
TTTGAATCCA GCACTTCAGA GTCCTCAGTG CTATCAAGTA GTAGTCCAGA AAGTACCCTG 
GTGAAGACAG AGCCTAATGG GATAACCATC CCTGGCATCT CATCAGATAT GCCAGATAGT 
GCCCACAAAA CTCCTGCCTC AGAAGCAAAG TCAGAAACTG GGCAGCCTAC CAAGGTTGGA 
CGTTTTCAGG TGACAACTAC AGCAAACAAA GTAGGTCGTT TCTCTGTGTC AAGAACTGAA 
GACAAGCTCA TTGAGGCAAA GAAAGAGGGA CCAATAGCGT CTCCTCCTTT TATGGATTTG 
GAACAAGGTG TTCTTCCAGC TGTGATACCA AAGAAAGAAA AGCCTGAACT GTCAGAGCCT 
TCACATCTGA ACGGACCATC TTCTGACCTG GAGGCTGCCT TCCTGAGCAG GGATGTGGAT 
GATGGTTCAG GTAGTCCACA CTCCCCCCAT CAGCTGAGCT CAAAGAGCCT GCCTATCCAG 
AATCTAAGTC AAAGCCTTAG TAATTCATTC AACTCCTCTT ACATGAGTAG TGACAATGAG 
TCAGATATTG AAGATGAAGA TTTAAAATTA GAGCTGCGAA GACTACGAGA AAAACATCTT 
AAAGAGATTC AAGACCTGCA GAGTCGCCAG AAGCATGAAA TTGAATCTTT GTATACCAAA 
CTGGGAAAGG TTCCCCCTGC TGTCATTATC CCCCCAGCTG CCCCCCTTTC AGGGAGAAGA 
AGGCGACCCA CTAAAAGCAA AGGCAGCAAG TCTAGTCGCA GCAGTTCCTT GGGGAATAAA 
AGTCCCCAGC TTTCAGGTAA CCTGTCTGGT CAGAGTGCTG CTTCAGTTTT GCACGCTCAG 
CAGACCCTCC ACCCTCCTGG CAACATCCCT GAGACTGGGC CAAATCAGCT GTTACAGCCC 
CTTAAGCCAT CTCCCTCCAG TGACAACCTC TACTCAGCCT TTACCAGTGA TGGTGCCATT 
TCAGTCCCAA GCCTTTCTGC TCCAGGTCAA GGCTGTGCGA AGTTTAACTG TGCATCTGAG 
CAGGTGACAT TCAAACCTGG TGGCAGGAGG ACCCGATTTC TGAGTACGCC CTGCTTGGCT 
CTTTGGAAGA TGGTGAAAAA AGTCTGTCCT TGCAACCAGC TCTGTATGTG CCCTCCAGCA 
GAGCCGAAAT CAGGGACCAG CAGCACAAAC ACTGTCGGGG GAACAGTGAA CAGCCAAGCT 
GCCCCAGCTC AGCCTCCTGC CATGACATCC AGCAGGAAGG GCACATTCAC AGATGACCTA 
CACAAGTTGG TAGACAATTG GGCCCGAGAT GCCATGAATC TCTCAGGCAG AAGAGGAAGC 
AAAGGACACA TGAATTATGA GGGCCCTGGA ATGGCAAGAA AGTTCTCCGC ACCTGGTCAG 
CTGTGCATCT CCATGACCTC AAATCTGGGT GGTTCTGCCC CCATCTCTGC AGCATCAGCT 
ACCTCTCTAG GTCACTTCAC CAAGTCTATG TGCCCCCCAC AGCAGTACGG TTTTCCAGCT 
CCCCCCTTTG GCACTCAGTG GAGTGGGACG GGTGGCCCGG CACCACAGCC ACTTGGCCAG 
TTCCAGCCTG TGGGAACTGC CTCCTTACAG AATTTCAACA TCAGCAATTT GCAGAAATCC 
ATCAGCAACC CCCCAGGTTC CAACCTGCGG ACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963806.1
CDS	99..7397
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQLPVSQPVPTIQGEPQIPVAAQPSVVPVHSGAHFLPVGQPLSTSLLPQYPVSQIPISTPHASAPQTGFSSLPITMAAGINQPLLTLASSATAAAIPGGPTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPSRLPPQYPGDSNIAPSSSVASICIPSTVLSPPMPTEALATPGYFPTVVQPFVESNLLVPVGSIGGQVQVSQPAVSLAQQAPTTSSQQAALEQNEMPMWGPCCHWLFCWESTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGCAKFNCASEQVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCMCPPAEPKSGTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_014108331.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108331.1

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGCAG 
GGAGTACAGC AGACAGCCCC TCCACAACAG ACAGCGCAGT ATTCGCTTCC ACAGACATCA 
GCCTCCAGTG AGGCCACTAT TGCACAACCA GTCAGTCAGT CTCAGGCTCC ACAGGTCTTG 
CCTCAAATAT CAGCTGGAAA ACAGCTTCCA GTTTCCCAGC CAGTACCAAC TATCCAAGGC 
GAACCTCAGA TCCCAGTTGC GGCACAACCC TCAGTTGTTC CAGTCCACTC TGGTGCTCAT 
TTCCTCCCTG TGGGACAGCC ACTCTCTACT TCCTTACTCC CTCAGTACCC GGTCTCTCAA 
ATTCCCATAT CAACTCCTCA TGCGTCTGCG CCCCAGACAG GTTTTTCATC CCTTCCCATT 
ACAATGGCAG CTGGCATTAA TCAGCCTCTG CTCACCTTGG CTTCATCTGC TACAGCAGCT 
GCGATCCCAG GGGGACCAAC TGTGGTTCCT AGTCAGCTTC CAACCCTTCT GCAGCCTGTG 
ACTCAGCTGC CAAGTCAGGT TCACCCACAA CTCCTGCAAC CAGCAGTTCA GTCCATGGGA 
ATACCAGCTA ACCTTGGACA AGCTGCTGAG GTTCCACTTC CCTCTGGAGA TGTTCTGTAC 
CAGGGCTTTC CATCTCGACT GCCACCACAG TACCCAGGAG ATTCAAATAT TGCTCCTTCT 
TCCAGCGTGG CTTCTATTTG CATCCCTTCT ACAGTCCTAT CCCCTCCCAT GCCCACAGAA 
GCACTGGCTA CACCGGGTTA CTTTCCTACA GTGGTGCAGC CTTTTGTGGA ATCTAACCTT 
TTGGTTCCTG TGGGCAGTAT AGGAGGACAG GTTCAAGTGT CCCAGCCAGC AGTGAGTTTA 
GCACAACAAG CCCCCACTAC ATCCTCCCAG CAAGCAGCTC TGGAGCAGAA TGAAATGCCA 
ATGTGGGGGC CTTGTTGCCA CTGGCTGTTC TGTTGGGAAA GCACTCAGGG AGTCTCTCAA 
GTTGCTCCTC CAGAGCCAGT TCCAGTGGCA CAGGCACAGC CTACCACTTT GGTCTCCTCT 
ATAGACAGTG CACATTCAGA TGTTGCTTCA GGAATGAGTG ATGGCAATGA GAATGTCCCG 
TCCTCCAGTG GAAGGCATGA AGGGAGAGCT ACAAAACGAC ATTACCGAAA ATCTGTAAGA 
AGTCGCTCTC GTCATGAAAA GACTTCGCGT CCAAAATTAA GAATTTTGAA TGTTTCAAAT 
AAAGGAGACC GAGTAGTAGA ATGTCAACTA GAGACTCATA ATCGGAAAAT GGTTACATTC 
AAATTTGATT TAGATGGTGA CAATCCTGAG GAGATAGCAA CAATTATGGT GAACAATGAC 
TTTATTCTAG CAATGGAGAG AGAGTCCTTT GTGGATCAAG TACGAGAAAT TATTGAAAAG 
GCTGATGAAA TGCTCAGTGA GGATGTCAGT GTGGAACCAG AGGGTGACCA GGGATTGGAG 
AGTCTGCAGG GAAAGGATGA CTATGGCTTT ACAGGTTCTC AAAAATTGGA AGGAGAATTC 
AAACAACCAG TTCCTGCGTC TTCCATGCCA CAGCAAATAG GCATTCCTAC CAGTTCTTTA 
ACTCAAGTTG TTCATTCTGC TGGAAGACGG TTTATAGTGA GTCCTGTGCC AGAAAGTCGA 
TTACGAGAAT CAAAAGTTTT CACTAGTGAA ATACCAGATA CAGTTGCTGC CTCTACTCAC 
AGCGCTGGAA TGAACTTGTC TCACTCTGCT TCATCCCTTA GCCTACAGCA AGCCTTTTCT 
GAACTTAGAC ATGTGCAGAT GACAGAAGGG CCCAGCACAG CACCTCCCAA CTTCAGTCAT 
ACAGGACCAA CATTTCCAGT TGTTCCTCCT TCCATGAGTA GCATTGCTGG AGTCCCAACC 
ACAGCAGTAG CCACACCTTC AGTATCAGTC CCTGCAACTA GCAGCCCTCT AAATGACAGT 
TCCACATCAG TAATTCAATC TGAGATTCCA GTGCCCACTG AAACAGGGGT TGGTGGAGTT 
CCCACCTGCA CAAGTGTAAT GCCTTCAAGT GGTCTCCCTG TACCACCCAT ATCTGAATCG 
CCAATATTTT CCACTATGGT TTCAAGCATC ACAGTACCTA CAGTTGTCTC AATATCAGCA 
ACATCCCAGC CAGTTCAAGC TCCCACATCT GGGAGCACTG TTTCTAGCAT AGGAACTTTG 
CCTTCTATAC CAGTTTCAAC AGCTTTAGCC TCTGCAGTGG GCAGTGTTGC TATCCCAGGT 
GCTAAGCCTC CACCTGTACT ATCACAGCAG GCAGCAGGCA GTACTACTGG GGCAGCCACA 
TTAACATCCG TTCCTGCCAT CACTCCGTTC CCAAGCATAG CTTCACAGCC ATCTCTTCAG 
CTTAGCAGCA GCACCTCTGC TCCAACTCTA GCTGAAACAG TGGTGGTTAG TGCACACTCA 
CTAGATAAAA CATCTCATAG CAGTACAACT GGATTGGCTT TGTCCCTGTC TGCATCATCT 
TCCTCCCCTG GAGCAGGATT ATCTAGTTCT ATTTCTCAGC CTGGTGGGGT GCATCCTTTG 
GTTACCCCAT CAGCTATGGC TTCTATTCCT GCCCTTCCTC AAACAGCAGG ACCTACTTCT 
ACACCTTTAT TGCCCCAAGT ACTTAGTATC CCACCCTTGG TACAACCTGT TGCCAGTGTG 
CCTGCTGTGC AGCAGACACT AATTCATAGT CAGCCTCAAC CAGCTTTACT TCCCAACCAG 
CCCCACACTC ACTGTCCTGA AATAGATGCT GACTTACAGC CCAAAGCTCC TGGAATTGAT 
GACATAAAGA CTCTAGAGGA AAAGCTGCGG TCTCTCTTTA GTGAACCGAG CTCATCTGGA 
GCCCAACATA CATCGGTCTC ACTAGAGACT TCACTGGTAG TAGAGACCAC TGTCACGCCA 
GGTATCCCAA CCACTGCTAT TGCACCAAGC AAGCTCATGA CATCTACTAC AAGTACATGC 
TTACCACCAA CCAGTTTGCA ATTAGGAACA TCTGGTTTGT CAGTTATACC AGTGGTCACG 
CCTGGGCAGG TTTCTACCCC AGTCAGCTAT GTGTCAGCCC CAGGCAGCAC TACATCAGGA 
GTGAAAGCTG GAACTGCTCC ATCAAAGCCA CCTTTGACTA AAGCTCCAGT GCTGCCAGTG 
GGTACTGAAC TTCCAGCTGG TACTCCACCC AGCGAGCAGC TGCCACCTTT TCCAGGACCT 
TCACTAACTC AGTCCCAGCA ACCTCTGGAA GATCTTGATG CTCAGTTGAG AAGAACACTT 
AGTCCAGAGA CTATTGCGGT GACATCTACA ATTGGTCCTG TGTCCATGGT GGCTCCAACA 
GCAGTCACAG AAGCAGGAGC ACAGCCTCAG AAGGATGTTT CTCAGATCAC AGAAGGTCCT 
GTCCTAGCGA CTAGTTCGGG AACTGGTGTT TTTAAGATGG GAAGATTTCA GGTTTCAGTT 
GCAATGGATG ATGTTCAAAA GGAGGGTAAA AATAAGTTAG AAGATGGAAA GTCTGTTCAT 
TTTGAATCCA GCACTTCAGA GTCCTCAGTG CTATCAAGTA GTAGTCCAGA AAGTACCCTG 
GTGAAGACAG AGCCTAATGG GATAACCATC CCTGGCATCT CATCAGATAT GCCAGATAGT 
GCCCACAAAA CTCCTGCCTC AGAAGCAAAG TCAGAAACTG GGCAGCCTAC CAAGGTTGGA 
CGTTTTCAGG TGACAACTAC AGCAAACAAA GTAGGTCGTT TCTCTGTGTC AAGAACTGAA 
GACAAGCTCA TTGAGGCAAA GAAAGAGGGA CCAATAGCGT CTCCTCCTTT TATGGATTTG 
GAACAAGGTG TTCTTCCAGC TGTGATACCA AAGAAAGAAA AGCCTGAACT GTCAGAGCCT 
TCACATCTGA ACGGACCATC TTCTGACCTG GAGGCTGCCT TCCTGAGCAG GGATGTGGAT 
GATGGTTCAG GTAGTCCACA CTCCCCCCAT CAGCTGAGCT CAAAGAGCCT GCCTATCCAG 
AATCTAAGTC AAAGCCTTAG TAATTCATTC AACTCCTCTT ACATGAGTAG TGACAATGAG 
TCAGATATTG AAGATGAAGA TTTAAAATTA GAGCTGCGAA GACTACGAGA AAAACATCTT 
AAAGAGATTC AAGACCTGCA GAGTCGCCAG AAGCATGAAA TTGAATCTTT GTATACCAAA 
CTGGGAAAGG TTCCCCCTGC TGTCATTATC CCCCCAGCTG CCCCCCTTTC AGGGAGAAGA 
AGGCGACCCA CTAAAAGCAA AGGCAGCAAG TCTAGTCGCA GCAGTTCCTT GGGGAATAAA 
AGTCCCCAGC TTTCAGGTAA CCTGTCTGGT CAGAGTGCTG CTTCAGTTTT GCACGCTCAG 
CAGACCCTCC ACCCTCCTGG CAACATCCCT GAGACTGGGC CAAATCAGCT GTTACAGCCC 
CTTAAGCCAT CTCCCTCCAG TGACAACCTC TACTCAGCCT TTACCAGTGA TGGTGCCATT 
TCAGTCCCAA GCCTTTCTGC TCCAGGTCAA GGCTGTGCGA AGTTTAACTG TGCATCTGAG 
CAGGTGACAT TCAAACCTGG TGGCAGGAGG ACCCGATTTC TGAGTACGCC CTGCTTGGCT 
CTTTGGAAGA TGGTGAAAAA AGTCTGTCCT TGCAACCAGC TCTGTATGTG CCCTCCAGCA 
GAGCCGAAAT CAGGGACCAG CAGCACAAAC ACTGTCGGGG GAACAGTGAA CAGCCAAGCT 
GCCCCAGCTC AGCCTCCTGC CATGACATCC AGCAGGAAGG GCACATTCAC AGATGACCTA 
CACAAGTTGG TAGACAATTG GGCCCGAGAT GCCATGAATC TCTCAGGCAG AAGAGGAAGC 
AAAGGACACA TGAATTATGA GGGCCCTGGA ATGGCAAGAA AGTTCTCCGC ACCTGGTCAG 
CTGTGCATCT CCATGACCTC AAATCTGGGT GGTTCTGCCC CCATCTCTGC AGCATCAGCT 
ACCTCTCTAG GTCACTTCAC CAAGTCTATG TGCCCCCCAC AGCAGTACGG TTTTCCAGCT 
CCCCCCTTTG GCACTCAGTG GAGTGGGACG GGTGGCCCGG CACCACAGCC ACTTGGCCAG 
TTCCAGCCTG TGGGAACTGC CTCCTTACAG AATTTCAACA TCAGCAATTT GCAGAAATCC 
ATCAGCAACC CCCCAGGTTC CAACCTGCGG ACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_014108332.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963807.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X9, mRNA.

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CloneID	OCe43577
Clone ID Related Accession (Same CDS sequence)	XM_014108332.1
Accession Version	XM_014108332.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase WNK1 isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG 
CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT 
CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC 
CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA 
GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG 
GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT 
CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA 
CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT 
CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA 
GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA 
TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG 
CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG 
TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT 
CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA 
AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG 
CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT 
GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA 
CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT 
AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA 
GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT 
CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG 
AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT 
ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT 
AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA 
GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT 
AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT 
AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT 
GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT 
GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT 
ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT 
GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG 
GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC 
AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA 
GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA 
GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT 
TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG 
CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT 
CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG 
GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG 
CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT 
AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA 
GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG 
ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG 
TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC 
CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT 
AAAGCTCCAT CCCAGCAACC TCTGGAAGAT CTTGATGCTC AGTTGAGAAG AACACTTAGT 
CCAGAGACTA TTGCGGTGAC ATCTACAATT GGTCCTGTGT CCATGGTGGC TCCAACAGCA 
GTCACAGAAG CAGGAGCACA GCCTCAGAAG GATGTTTCTC AGATCACAGA AGGTCCTGTC 
CTAGCGACTA GTTCGGGAAC TGGTGTTTTT AAGATGGGAA GATTTCAGGT TTCAGTTGCA 
ATGGATGATG TTCAAAAGGA GGGTAAAAAT AAGTTAGAAG ATGGAAAGTC TGTTCATTTT 
GAATCCAGCA CTTCAGAGTC CTCAGTGCTA TCAAGTAGTA GTCCAGAAAG TACCCTGGTG 
AAGACAGAGC CTAATGGGAT AACCATCCCT GGCATCTCAT CAGATATGCC AGATAGTGCC 
CACAAAACTC CTGCCTCAGA AGCAAAGTCA GAAACTGGGC AGCCTACCAA GGTTGGACGT 
TTTCAGGTGA CAACTACAGC AAACAAAGTA GGTCGTTTCT CTGTGTCAAG AACTGAAGAC 
AAGCTCATTG AGGCAAAGAA AGAGGGACCA ATAGCGTCTC CTCCTTTTAT GGATTTGGAA 
CAAGGTGTTC TTCCAGCTGT GATACCAAAG AAAGAAAAGC CTGAACTGTC AGAGCCTTCA 
CATCTGAACG GACCATCTTC TGACCTGGAG GCTGCCTTCC TGAGCAGGGA TGTGGATGAT 
GGTTCAGGTA GTCCACACTC CCCCCATCAG CTGAGCTCAA AGAGCCTGCC TATCCAGAAT 
CTAAGTCAAA GCCTTAGTAA TTCATTCAAC TCCTCTTACA TGAGTAGTGA CAATGAGTCA 
GATATTGAAG ATGAAGATTT AAAATTAGAG CTGCGAAGAC TACGAGAAAA ACATCTTAAA 
GAGATTCAAG ACCTGCAGAG TCGCCAGAAG CATGAAATTG AATCTTTGTA TACCAAACTG 
GGAAAGGTTC CCCCTGCTGT CATTATCCCC CCAGCTGCCC CCCTTTCAGG GAGAAGAAGG 
CGACCCACTA AAAGCAAAGG CAGCAAGTCT AGTCGCAGCA GTTCCTTGGG GAATAAAAGT 
CCCCAGCTTT CAGGTAACCT GTCTGGTCAG AGTGCTGCTT CAGTTTTGCA CGCTCAGCAG 
ACCCTCCACC CTCCTGGCAA CATCCCTGAG ACTGGGCCAA ATCAGCTGTT ACAGCCCCTT 
AAGCCATCTC CCTCCAGTGA CAACCTCTAC TCAGCCTTTA CCAGTGATGG TGCCATTTCA 
GTCCCAAGCC TTTCTGCTCC AGGTCAAGGC TGTGCGAAGT TTAACTGTGC ATCTGAGCAG 
GTGACATTCA AACCTGGTGG CAGGAGGACC CGATTTCTGA GTACGCCCTG CTTGGCTCTT 
TGGAAGATGG TGAAAAAAGT CTGTCCTTGC AACCAGCTCT GTATGTGCCC TCCAGCAGAG 
CCGAAATCAG GGACCAGCAG CACAAACACT GTCGGGGGAA CAGTGAACAG CCAAGCTGCC 
CCAGCTCAGC CTCCTGCCAT GACATCCAGC AGGAAGGGCA CATTCACAGA TGACCTACAC 
AAGTTGGTAG ACAATTGGGC CCGAGATGCC ATGAATCTCT CAGGCAGAAG AGGAAGCAAA 
GGACACATGA ATTATGAGGG CCCTGGAATG GCAAGAAAGT TCTCCGCACC TGGTCAGCTG 
TGCATCTCCA TGACCTCAAA TCTGGGTGGT TCTGCCCCCA TCTCTGCAGC ATCAGCTACC 
TCTCTAGGTC ACTTCACCAA GTCTATGTGC CCCCCACAGC AGTACGGTTT TCCAGCTCCC 
CCCTTTGGCA CTCAGTGGAG TGGGACGGGT GGCCCGGCAC CACAGCCACT TGGCCAGTTC 
CAGCCTGTGG GAACTGCCTC CTTACAGAAT TTCAACATCA GCAATTTGCA GAAATCCATC 
AGCAACCCCC CAGGTTCCAA CCTGCGGACC ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963807.1
CDS	99..7394
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVGQSQGQPSSSSLTGIPSSLPIQHSQQQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQLPVSQPVPTIQGEPQIPVAAQPSVVPVHSGAHFLPVGQPLSTSLLPQYPVSQIPISTPHASAPQTGFSSLPITMAAGINQPLLTLASSATAAAIPGGPTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPSRLPPQYPGDSNIAPSSSVASICIPSTVLSPPMPTEALATPGYFPTVVQPFVESNLLVPVGSIGGQVQVSQPAVSLAQQAPTTSSQQAALEQNEMPMWGPCCHWLFCWESTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGCAKFNCASEQVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCMCPPAEPKSGTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_014108332.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108332.1

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG 
CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT 
CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC 
CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA 
GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG 
GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT 
CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA 
CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT 
CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA 
GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA 
TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG 
CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG 
TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT 
CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA 
AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG 
CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT 
GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA 
CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT 
AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA 
GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT 
CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG 
AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT 
ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT 
AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA 
GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT 
AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT 
AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT 
GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT 
GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT 
ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT 
GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG 
GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC 
AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA 
GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA 
GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT 
TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG 
CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT 
CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG 
GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG 
CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT 
AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA 
GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG 
ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG 
TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC 
CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT 
AAAGCTCCAT CCCAGCAACC TCTGGAAGAT CTTGATGCTC AGTTGAGAAG AACACTTAGT 
CCAGAGACTA TTGCGGTGAC ATCTACAATT GGTCCTGTGT CCATGGTGGC TCCAACAGCA 
GTCACAGAAG CAGGAGCACA GCCTCAGAAG GATGTTTCTC AGATCACAGA AGGTCCTGTC 
CTAGCGACTA GTTCGGGAAC TGGTGTTTTT AAGATGGGAA GATTTCAGGT TTCAGTTGCA 
ATGGATGATG TTCAAAAGGA GGGTAAAAAT AAGTTAGAAG ATGGAAAGTC TGTTCATTTT 
GAATCCAGCA CTTCAGAGTC CTCAGTGCTA TCAAGTAGTA GTCCAGAAAG TACCCTGGTG 
AAGACAGAGC CTAATGGGAT AACCATCCCT GGCATCTCAT CAGATATGCC AGATAGTGCC 
CACAAAACTC CTGCCTCAGA AGCAAAGTCA GAAACTGGGC AGCCTACCAA GGTTGGACGT 
TTTCAGGTGA CAACTACAGC AAACAAAGTA GGTCGTTTCT CTGTGTCAAG AACTGAAGAC 
AAGCTCATTG AGGCAAAGAA AGAGGGACCA ATAGCGTCTC CTCCTTTTAT GGATTTGGAA 
CAAGGTGTTC TTCCAGCTGT GATACCAAAG AAAGAAAAGC CTGAACTGTC AGAGCCTTCA 
CATCTGAACG GACCATCTTC TGACCTGGAG GCTGCCTTCC TGAGCAGGGA TGTGGATGAT 
GGTTCAGGTA GTCCACACTC CCCCCATCAG CTGAGCTCAA AGAGCCTGCC TATCCAGAAT 
CTAAGTCAAA GCCTTAGTAA TTCATTCAAC TCCTCTTACA TGAGTAGTGA CAATGAGTCA 
GATATTGAAG ATGAAGATTT AAAATTAGAG CTGCGAAGAC TACGAGAAAA ACATCTTAAA 
GAGATTCAAG ACCTGCAGAG TCGCCAGAAG CATGAAATTG AATCTTTGTA TACCAAACTG 
GGAAAGGTTC CCCCTGCTGT CATTATCCCC CCAGCTGCCC CCCTTTCAGG GAGAAGAAGG 
CGACCCACTA AAAGCAAAGG CAGCAAGTCT AGTCGCAGCA GTTCCTTGGG GAATAAAAGT 
CCCCAGCTTT CAGGTAACCT GTCTGGTCAG AGTGCTGCTT CAGTTTTGCA CGCTCAGCAG 
ACCCTCCACC CTCCTGGCAA CATCCCTGAG ACTGGGCCAA ATCAGCTGTT ACAGCCCCTT 
AAGCCATCTC CCTCCAGTGA CAACCTCTAC TCAGCCTTTA CCAGTGATGG TGCCATTTCA 
GTCCCAAGCC TTTCTGCTCC AGGTCAAGGC TGTGCGAAGT TTAACTGTGC ATCTGAGCAG 
GTGACATTCA AACCTGGTGG CAGGAGGACC CGATTTCTGA GTACGCCCTG CTTGGCTCTT 
TGGAAGATGG TGAAAAAAGT CTGTCCTTGC AACCAGCTCT GTATGTGCCC TCCAGCAGAG 
CCGAAATCAG GGACCAGCAG CACAAACACT GTCGGGGGAA CAGTGAACAG CCAAGCTGCC 
CCAGCTCAGC CTCCTGCCAT GACATCCAGC AGGAAGGGCA CATTCACAGA TGACCTACAC 
AAGTTGGTAG ACAATTGGGC CCGAGATGCC ATGAATCTCT CAGGCAGAAG AGGAAGCAAA 
GGACACATGA ATTATGAGGG CCCTGGAATG GCAAGAAAGT TCTCCGCACC TGGTCAGCTG 
TGCATCTCCA TGACCTCAAA TCTGGGTGGT TCTGCCCCCA TCTCTGCAGC ATCAGCTACC 
TCTCTAGGTC ACTTCACCAA GTCTATGTGC CCCCCACAGC AGTACGGTTT TCCAGCTCCC 
CCCTTTGGCA CTCAGTGGAG TGGGACGGGT GGCCCGGCAC CACAGCCACT TGGCCAGTTC 
CAGCCTGTGG GAACTGCCTC CTTACAGAAT TTCAACATCA GCAATTTGCA GAAATCCATC 
AGCAACCCCC CAGGTTCCAA CCTGCGGACC ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_014108333.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963808.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X10, mRNA.

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CloneID	OCe43578
Clone ID Related Accession (Same CDS sequence)	XM_014108333.1
Accession Version	XM_014108333.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase WNK1 isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG 
CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT 
CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC 
CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA 
GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG 
GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT 
CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA 
CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT 
CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA 
GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA 
TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG 
CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG 
TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT 
CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA 
AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG 
CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT 
GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA 
CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT 
AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA 
GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT 
CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG 
AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT 
ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT 
AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA 
GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT 
AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT 
AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT 
GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT 
GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT 
ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT 
GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG 
GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC 
AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA 
GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA 
GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT 
TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG 
CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT 
CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG 
GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG 
CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT 
AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA 
GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG 
ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG 
TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC 
CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT 
AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG 
CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT 
GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT 
GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT 
TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG 
GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA 
GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT 
AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC 
TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT 
GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT 
TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG 
TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA 
AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC 
TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC 
TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT 
TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA 
AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA 
ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT 
GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC 
AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT 
GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG 
CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC 
TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGGAAGATG 
GTGAAAAAAG TCTGTCCTTG CAACCAGCTC TGTATGTGCC CTCCAGCAGA GCCGAAATCA 
GGGACCAGCA GCACAAACAC TGTCGGGGGA ACAGTGAACA GCCAAGCTGC CCCAGCTCAG 
CCTCCTGCCA TGACATCCAG CAGGAAGGGC ACATTCACAG ATGACCTACA CAAGTTGGTA 
GACAATTGGG CCCGAGATGC CATGAATCTC TCAGGCAGAA GAGGAAGCAA AGGACACATG 
AATTATGAGG GCCCTGGAAT GGCAAGAAAG TTCTCCGCAC CTGGTCAGCT GTGCATCTCC 
ATGACCTCAA ATCTGGGTGG TTCTGCCCCC ATCTCTGCAG CATCAGCTAC CTCTCTAGGT 
CACTTCACCA AGTCTATGTG CCCCCCACAG CAGTACGGTT TTCCAGCTCC CCCCTTTGGC 
ACTCAGTGGA GTGGGACGGG TGGCCCGGCA CCACAGCCAC TTGGCCAGTT CCAGCCTGTG 
GGAACTGCCT CCTTACAGAA TTTCAACATC AGCAATTTGC AGAAATCCAT CAGCAACCCC 
CCAGGTTCCA ACCTGCGGAC CACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963808.1
CDS	99..7385
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVGQSQGQPSSSSLTGIPSSLPIQHSQQQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQLPVSQPVPTIQGEPQIPVAAQPSVVPVHSGAHFLPVGQPLSTSLLPQYPVSQIPISTPHASAPQTGFSSLPITMAAGINQPLLTLASSATAAAIPGGPTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPSRLPPQYPGDSNIAPSSSVASICIPSTVLSPPMPTEALATPGYFPTVVQPFVESNLLVPVGSIGGQVQVSQPAVSLAQQAPTTSSQQAALEQNEMPMWGPCCHWLFCWESTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGKMVKKVCPCNQLCMCPPAEPKSGTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_014108333.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108333.1

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG 
CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT 
CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC 
CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA 
GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG 
GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT 
CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA 
CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT 
CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA 
GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA 
TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG 
CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG 
TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT 
CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA 
AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG 
CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT 
GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA 
CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT 
AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA 
GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT 
CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG 
AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT 
ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT 
AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA 
GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT 
AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT 
AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT 
GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT 
GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT 
ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT 
GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG 
GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC 
AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA 
GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA 
GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT 
TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG 
CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT 
CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG 
GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG 
CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT 
AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA 
GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG 
ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG 
TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC 
CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT 
AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG 
CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT 
GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT 
GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT 
TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG 
GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA 
GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT 
AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC 
TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT 
GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT 
TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG 
TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA 
AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC 
TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC 
TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT 
TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA 
AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA 
ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT 
GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC 
AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT 
GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG 
CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC 
TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGGAAGATG 
GTGAAAAAAG TCTGTCCTTG CAACCAGCTC TGTATGTGCC CTCCAGCAGA GCCGAAATCA 
GGGACCAGCA GCACAAACAC TGTCGGGGGA ACAGTGAACA GCCAAGCTGC CCCAGCTCAG 
CCTCCTGCCA TGACATCCAG CAGGAAGGGC ACATTCACAG ATGACCTACA CAAGTTGGTA 
GACAATTGGG CCCGAGATGC CATGAATCTC TCAGGCAGAA GAGGAAGCAA AGGACACATG 
AATTATGAGG GCCCTGGAAT GGCAAGAAAG TTCTCCGCAC CTGGTCAGCT GTGCATCTCC 
ATGACCTCAA ATCTGGGTGG TTCTGCCCCC ATCTCTGCAG CATCAGCTAC CTCTCTAGGT 
CACTTCACCA AGTCTATGTG CCCCCCACAG CAGTACGGTT TTCCAGCTCC CCCCTTTGGC 
ACTCAGTGGA GTGGGACGGG TGGCCCGGCA CCACAGCCAC TTGGCCAGTT CCAGCCTGTG 
GGAACTGCCT CCTTACAGAA TTTCAACATC AGCAATTTGC AGAAATCCAT CAGCAACCCC 
CCAGGTTCCA ACCTGCGGAC CACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_014108334.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963809.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X11, mRNA.

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CloneID	OCe43579
Clone ID Related Accession (Same CDS sequence)	XM_014108334.1 , XM_014108334.2
Accession Version	XM_014108334.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase WNK1 isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG 
CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT 
CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC 
CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA 
GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG 
GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT 
CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA 
CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT 
CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA 
GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA 
TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG 
CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG 
TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT 
CTGGAGAGCA CTCAGGGAGT CTCTCAAGTT GCTCCTCCAG AGCCAGTTCC AGTGGCACAG 
GCACAGCCTA CCACTTTGGT CTCCTCTATA GACAGTGCAC ATTCAGATGT TGCTTCAGGA 
ATGAGTGATG GCAATGAGAA TGTCCCGTCC TCCAGTGGAA GGCATGAAGG GAGAGCTACA 
AAACGACATT ACCGAAAATC TGTAAGAAGT CGCTCTCGTC ATGAAAAGAC TTCGCGTCCA 
AAATTAAGAA TTTTGAATGT TTCAAATAAA GGAGACCGAG TAGTAGAATG TCAACTAGAG 
ACTCATAATC GGAAAATGGT TACATTCAAA TTTGATTTAG ATGGTGACAA TCCTGAGGAG 
ATAGCAACAA TTATGGTGAA CAATGACTTT ATTCTAGCAA TGGAGAGAGA GTCCTTTGTG 
GATCAAGTAC GAGAAATTAT TGAAAAGGCT GATGAAATGC TCAGTGAGGA TGTCAGTGTG 
GAACCAGAGG GTGACCAGGG ATTGGAGAGT CTGCAGGGAA AGGATGACTA TGGCTTTACA 
GGTTCTCAAA AATTGGAAGG AGAATTCAAA CAACCAGTTC CTGCGTCTTC CATGCCACAG 
CAAATAGGCA TTCCTACCAG TTCTTTAACT CAAGTTGTTC ATTCTGCTGG AAGACGGTTT 
ATAGTGAGTC CTGTGCCAGA AAGTCGATTA CGAGAATCAA AAGTTTTCAC TAGTGAAATA 
CCAGATACAG TTGCTGCCTC TACTCACAGC GCTGGAATGA ACTTGTCTCA CTCTGCTTCA 
TCCCTTAGCC TACAGCAAGC CTTTTCTGAA CTTAGACATG TGCAGATGAC AGAAGGGCCC 
AGCACAGCAC CTCCCAACTT CAGTCATACA GGACCAACAT TTCCAGTTGT TCCTCCTTCC 
ATGAGTAGCA TTGCTGGAGT CCCAACCACA GCAGTAGCCA CACCTTCAGT ATCAGTCCCT 
GCAACTAGCA GCCCTCTAAA TGACAGTTCC ACATCAGTAA TTCAATCTGA GATTCCAGTG 
CCCACTGAAA CAGGGGTTGG TGGAGTTCCC ACCTGCACAA GTGTAATGCC TTCAAGTGGT 
CTCCCTGTAC CACCCATATC TGAATCGCCA ATATTTTCCA CTATGGTTTC AAGCATCACA 
GTACCTACAG TTGTCTCAAT ATCAGCAACA TCCCAGCCAG TTCAAGCTCC CACATCTGGG 
AGCACTGTTT CTAGCATAGG AACTTTGCCT TCTATACCAG TTTCAACAGC TTTAGCCTCT 
GCAGTGGGCA GTGTTGCTAT CCCAGGTGCT AAGCCTCCAC CTGTACTATC ACAGCAGGCA 
GCAGGCAGTA CTACTGGGGC AGCCACATTA ACATCCGTTC CTGCCATCAC TCCGTTCCCA 
AGCATAGCTT CACAGCCATC TCTTCAGCTT AGCAGCAGCA CCTCTGCTCC AACTCTAGCT 
GAAACAGTGG TGGTTAGTGC ACACTCACTA GATAAAACAT CTCATAGCAG TACAACTGGA 
TTGGCTTTGT CCCTGTCTGC ATCATCTTCC TCCCCTGGAG CAGGATTATC TAGTTCTATT 
TCTCAGCCTG GTGGGGTGCA TCCTTTGGTT ACCCCATCAG CTATGGCTTC TATTCCTGCC 
CTTCCTCAAA CAGCAGGACC TACTTCTACA CCTTTATTGC CCCAAGTACT TAGTATCCCA 
CCCTTGGTAC AACCTGTTGC CAGTGTGCCT GCTGTGCAGC AGACACTAAT TCATAGTCAG 
CCTCAACCAG CTTTACTTCC CAACCAGCCC CACACTCACT GTCCTGAAAT AGATGCTGAC 
TTACAGCCCA AAGCTCCTGG AATTGATGAC ATAAAGACTC TAGAGGAAAA GCTGCGGTCT 
CTCTTTAGTG AACCGAGCTC ATCTGGAGCC CAACATACAT CGGTCTCACT AGAGACTTCA 
CTGGTAGTAG AGACCACTGT CACGCCAGGT ATCCCAACCA CTGCTATTGC ACCAAGCAAG 
CTCATGACAT CTACTACAAG TACATGCTTA CCACCAACCA GTTTGCAATT AGGAACATCT 
GGTTTGTCAG TTATACCAGT GGTCACGCCT GGGCAGGTTT CTACCCCAGT CAGCTATGTG 
TCAGCCCCAG GCAGCACTAC ATCAGGAGTG AAAGCTGGAA CTGCTCCATC AAAGCCACCT 
TTGACTAAAG CTCCAGTGCT GCCAGTGGGT ACTGAACTTC CAGCTGGTAC TCCACCCAGC 
GAGCAGCTGC CACCTTTTCC AGGACCTTCA CTAACTCAGT CCCAGCAACC TCTGGAAGAT 
CTTGATGCTC AGTTGAGAAG AACACTTAGT CCAGAGACTA TTGCGGTGAC ATCTACAATT 
GGTCCTGTGT CCATGGTGGC TCCAACAGCA GTCACAGAAG CAGGAGCACA GCCTCAGAAG 
GATGTTTCTC AGATCACAGA AGGTCCTGTC CTAGCGACTA GTTCGGGAAC TGGTGTTTTT 
AAGATGGGAA GATTTCAGGT TTCAGTTGCA ATGGATGATG TTCAAAAGGA GGGTAAAAAT 
AAGTTAGAAG ATGGAAAGTC TGTTCATTTT GAATCCAGCA CTTCAGAGTC CTCAGTGCTA 
TCAAGTAGTA GTCCAGAAAG TACCCTGGTG AAGACAGAGC CTAATGGGAT AACCATCCCT 
GGCATCTCAT CAGATATGCC AGATAGTGCC CACAAAACTC CTGCCTCAGA AGCAAAGTCA 
GAAACTGGGC AGCCTACCAA GGTTGGACGT TTTCAGGTGA CAACTACAGC AAACAAAGTA 
GGTCGTTTCT CTGTGTCAAG AACTGAAGAC AAGCTCATTG AGGCAAAGAA AGAGGGACCA 
ATAGCGTCTC CTCCTTTTAT GGATTTGGAA CAAGGTGTTC TTCCAGCTGT GATACCAAAG 
AAAGAAAAGC CTGAACTGTC AGAGCCTTCA CATCTGAACG GACCATCTTC TGACCTGGAG 
GCTGCCTTCC TGAGCAGGGA TGTGGATGAT GGTTCAGGTA GTCCACACTC CCCCCATCAG 
CTGAGCTCAA AGAGCCTGCC TATCCAGAAT CTAAGTCAAA GCCTTAGTAA TTCATTCAAC 
TCCTCTTACA TGAGTAGTGA CAATGAGTCA GATATTGAAG ATGAAGATTT AAAATTAGAG 
CTGCGAAGAC TACGAGAAAA ACATCTTAAA GAGATTCAAG ACCTGCAGAG TCGCCAGAAG 
CATGAAATTG AATCTTTGTA TACCAAACTG GGAAAGGTTC CCCCTGCTGT CATTATCCCC 
CCAGCTGCCC CCCTTTCAGG GAGAAGAAGG CGACCCACTA AAAGCAAAGG CAGCAAGTCT 
AGTCGCAGCA GTTCCTTGGG GAATAAAAGT CCCCAGCTTT CAGGTAACCT GTCTGGTCAG 
AGTGCTGCTT CAGTTTTGCA CGCTCAGCAG ACCCTCCACC CTCCTGGCAA CATCCCTGAG 
ACTGGGCCAA ATCAGCTGTT ACAGCCCCTT AAGCCATCTC CCTCCAGTGA CAACCTCTAC 
TCAGCCTTTA CCAGTGATGG TGCCATTTCA GTCCCAAGCC TTTCTGCTCC AGGTCAAGGC 
TGTGCGAAGT TTAACTGTGC ATCTGAGCAG GTGACATTCA AACCTGGTGG CAGGAGGACC 
CGATTTCTGA GGAAGATGGT GAAAAAAGTC TGTCCTTGCA ACCAGCTCTG TAGGACCAGC 
AGCACAAACA CTGTCGGGGG AACAGTGAAC AGCCAAGCTG CCCCAGCTCA GCCTCCTGCC 
ATGACATCCA GCAGGAAGGG CACATTCACA GATGACCTAC ACAAGTTGGT AGACAATTGG 
GCCCGAGATG CCATGAATCT CTCAGGCAGA AGAGGAAGCA AAGGACACAT GAATTATGAG 
GGCCCTGGAA TGGCAAGAAA GTTCTCCGCA CCTGGTCAGC TGTGCATCTC CATGACCTCA 
AATCTGGGTG GTTCTGCCCC CATCTCTGCA GCATCAGCTA CCTCTCTAGG TCACTTCACC 
AAGTCTATGT GCCCCCCACA GCAGTACGGT TTTCCAGCTC CCCCCTTTGG CACTCAGTGG 
AGTGGGACGG GTGGCCCGGC ACCACAGCCA CTTGGCCAGT TCCAGCCTGT GGGAACTGCC 
TCCTTACAGA ATTTCAACAT CAGCAATTTG CAGAAATCCA TCAGCAACCC CCCAGGTTCC 
AACCTGCGGA CCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963809.1
CDS	99..7376
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVGQSQGQPSSSSLTGIPSSLPIQHSQQQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQLPVSQPVPTIQGEPQIPVAAQPSVVPVHSGAHFLPVGQPLSTSLLPQYPVSQIPISTPHASAPQTGFSSLPITMAAGINQPLLTLASSATAAAIPGGPTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPSRLPPQYPGDSNIAPSSSVASICIPSTVLSPPMPTEALATPGYFPTVVQPFVESNLLVPVGSIGGQVQVSQPAVSLAQQAPTTSSQQAALESTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGCAKFNCASEQVTFKPGGRRTRFLRKMVKKVCPCNQLCRTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_014108334.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108334.1

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG 
CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT 
CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC 
CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA 
GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG 
GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT 
CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA 
CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT 
CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA 
GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA 
TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG 
CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG 
TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT 
CTGGAGAGCA CTCAGGGAGT CTCTCAAGTT GCTCCTCCAG AGCCAGTTCC AGTGGCACAG 
GCACAGCCTA CCACTTTGGT CTCCTCTATA GACAGTGCAC ATTCAGATGT TGCTTCAGGA 
ATGAGTGATG GCAATGAGAA TGTCCCGTCC TCCAGTGGAA GGCATGAAGG GAGAGCTACA 
AAACGACATT ACCGAAAATC TGTAAGAAGT CGCTCTCGTC ATGAAAAGAC TTCGCGTCCA 
AAATTAAGAA TTTTGAATGT TTCAAATAAA GGAGACCGAG TAGTAGAATG TCAACTAGAG 
ACTCATAATC GGAAAATGGT TACATTCAAA TTTGATTTAG ATGGTGACAA TCCTGAGGAG 
ATAGCAACAA TTATGGTGAA CAATGACTTT ATTCTAGCAA TGGAGAGAGA GTCCTTTGTG 
GATCAAGTAC GAGAAATTAT TGAAAAGGCT GATGAAATGC TCAGTGAGGA TGTCAGTGTG 
GAACCAGAGG GTGACCAGGG ATTGGAGAGT CTGCAGGGAA AGGATGACTA TGGCTTTACA 
GGTTCTCAAA AATTGGAAGG AGAATTCAAA CAACCAGTTC CTGCGTCTTC CATGCCACAG 
CAAATAGGCA TTCCTACCAG TTCTTTAACT CAAGTTGTTC ATTCTGCTGG AAGACGGTTT 
ATAGTGAGTC CTGTGCCAGA AAGTCGATTA CGAGAATCAA AAGTTTTCAC TAGTGAAATA 
CCAGATACAG TTGCTGCCTC TACTCACAGC GCTGGAATGA ACTTGTCTCA CTCTGCTTCA 
TCCCTTAGCC TACAGCAAGC CTTTTCTGAA CTTAGACATG TGCAGATGAC AGAAGGGCCC 
AGCACAGCAC CTCCCAACTT CAGTCATACA GGACCAACAT TTCCAGTTGT TCCTCCTTCC 
ATGAGTAGCA TTGCTGGAGT CCCAACCACA GCAGTAGCCA CACCTTCAGT ATCAGTCCCT 
GCAACTAGCA GCCCTCTAAA TGACAGTTCC ACATCAGTAA TTCAATCTGA GATTCCAGTG 
CCCACTGAAA CAGGGGTTGG TGGAGTTCCC ACCTGCACAA GTGTAATGCC TTCAAGTGGT 
CTCCCTGTAC CACCCATATC TGAATCGCCA ATATTTTCCA CTATGGTTTC AAGCATCACA 
GTACCTACAG TTGTCTCAAT ATCAGCAACA TCCCAGCCAG TTCAAGCTCC CACATCTGGG 
AGCACTGTTT CTAGCATAGG AACTTTGCCT TCTATACCAG TTTCAACAGC TTTAGCCTCT 
GCAGTGGGCA GTGTTGCTAT CCCAGGTGCT AAGCCTCCAC CTGTACTATC ACAGCAGGCA 
GCAGGCAGTA CTACTGGGGC AGCCACATTA ACATCCGTTC CTGCCATCAC TCCGTTCCCA 
AGCATAGCTT CACAGCCATC TCTTCAGCTT AGCAGCAGCA CCTCTGCTCC AACTCTAGCT 
GAAACAGTGG TGGTTAGTGC ACACTCACTA GATAAAACAT CTCATAGCAG TACAACTGGA 
TTGGCTTTGT CCCTGTCTGC ATCATCTTCC TCCCCTGGAG CAGGATTATC TAGTTCTATT 
TCTCAGCCTG GTGGGGTGCA TCCTTTGGTT ACCCCATCAG CTATGGCTTC TATTCCTGCC 
CTTCCTCAAA CAGCAGGACC TACTTCTACA CCTTTATTGC CCCAAGTACT TAGTATCCCA 
CCCTTGGTAC AACCTGTTGC CAGTGTGCCT GCTGTGCAGC AGACACTAAT TCATAGTCAG 
CCTCAACCAG CTTTACTTCC CAACCAGCCC CACACTCACT GTCCTGAAAT AGATGCTGAC 
TTACAGCCCA AAGCTCCTGG AATTGATGAC ATAAAGACTC TAGAGGAAAA GCTGCGGTCT 
CTCTTTAGTG AACCGAGCTC ATCTGGAGCC CAACATACAT CGGTCTCACT AGAGACTTCA 
CTGGTAGTAG AGACCACTGT CACGCCAGGT ATCCCAACCA CTGCTATTGC ACCAAGCAAG 
CTCATGACAT CTACTACAAG TACATGCTTA CCACCAACCA GTTTGCAATT AGGAACATCT 
GGTTTGTCAG TTATACCAGT GGTCACGCCT GGGCAGGTTT CTACCCCAGT CAGCTATGTG 
TCAGCCCCAG GCAGCACTAC ATCAGGAGTG AAAGCTGGAA CTGCTCCATC AAAGCCACCT 
TTGACTAAAG CTCCAGTGCT GCCAGTGGGT ACTGAACTTC CAGCTGGTAC TCCACCCAGC 
GAGCAGCTGC CACCTTTTCC AGGACCTTCA CTAACTCAGT CCCAGCAACC TCTGGAAGAT 
CTTGATGCTC AGTTGAGAAG AACACTTAGT CCAGAGACTA TTGCGGTGAC ATCTACAATT 
GGTCCTGTGT CCATGGTGGC TCCAACAGCA GTCACAGAAG CAGGAGCACA GCCTCAGAAG 
GATGTTTCTC AGATCACAGA AGGTCCTGTC CTAGCGACTA GTTCGGGAAC TGGTGTTTTT 
AAGATGGGAA GATTTCAGGT TTCAGTTGCA ATGGATGATG TTCAAAAGGA GGGTAAAAAT 
AAGTTAGAAG ATGGAAAGTC TGTTCATTTT GAATCCAGCA CTTCAGAGTC CTCAGTGCTA 
TCAAGTAGTA GTCCAGAAAG TACCCTGGTG AAGACAGAGC CTAATGGGAT AACCATCCCT 
GGCATCTCAT CAGATATGCC AGATAGTGCC CACAAAACTC CTGCCTCAGA AGCAAAGTCA 
GAAACTGGGC AGCCTACCAA GGTTGGACGT TTTCAGGTGA CAACTACAGC AAACAAAGTA 
GGTCGTTTCT CTGTGTCAAG AACTGAAGAC AAGCTCATTG AGGCAAAGAA AGAGGGACCA 
ATAGCGTCTC CTCCTTTTAT GGATTTGGAA CAAGGTGTTC TTCCAGCTGT GATACCAAAG 
AAAGAAAAGC CTGAACTGTC AGAGCCTTCA CATCTGAACG GACCATCTTC TGACCTGGAG 
GCTGCCTTCC TGAGCAGGGA TGTGGATGAT GGTTCAGGTA GTCCACACTC CCCCCATCAG 
CTGAGCTCAA AGAGCCTGCC TATCCAGAAT CTAAGTCAAA GCCTTAGTAA TTCATTCAAC 
TCCTCTTACA TGAGTAGTGA CAATGAGTCA GATATTGAAG ATGAAGATTT AAAATTAGAG 
CTGCGAAGAC TACGAGAAAA ACATCTTAAA GAGATTCAAG ACCTGCAGAG TCGCCAGAAG 
CATGAAATTG AATCTTTGTA TACCAAACTG GGAAAGGTTC CCCCTGCTGT CATTATCCCC 
CCAGCTGCCC CCCTTTCAGG GAGAAGAAGG CGACCCACTA AAAGCAAAGG CAGCAAGTCT 
AGTCGCAGCA GTTCCTTGGG GAATAAAAGT CCCCAGCTTT CAGGTAACCT GTCTGGTCAG 
AGTGCTGCTT CAGTTTTGCA CGCTCAGCAG ACCCTCCACC CTCCTGGCAA CATCCCTGAG 
ACTGGGCCAA ATCAGCTGTT ACAGCCCCTT AAGCCATCTC CCTCCAGTGA CAACCTCTAC 
TCAGCCTTTA CCAGTGATGG TGCCATTTCA GTCCCAAGCC TTTCTGCTCC AGGTCAAGGC 
TGTGCGAAGT TTAACTGTGC ATCTGAGCAG GTGACATTCA AACCTGGTGG CAGGAGGACC 
CGATTTCTGA GGAAGATGGT GAAAAAAGTC TGTCCTTGCA ACCAGCTCTG TAGGACCAGC 
AGCACAAACA CTGTCGGGGG AACAGTGAAC AGCCAAGCTG CCCCAGCTCA GCCTCCTGCC 
ATGACATCCA GCAGGAAGGG CACATTCACA GATGACCTAC ACAAGTTGGT AGACAATTGG 
GCCCGAGATG CCATGAATCT CTCAGGCAGA AGAGGAAGCA AAGGACACAT GAATTATGAG 
GGCCCTGGAA TGGCAAGAAA GTTCTCCGCA CCTGGTCAGC TGTGCATCTC CATGACCTCA 
AATCTGGGTG GTTCTGCCCC CATCTCTGCA GCATCAGCTA CCTCTCTAGG TCACTTCACC 
AAGTCTATGT GCCCCCCACA GCAGTACGGT TTTCCAGCTC CCCCCTTTGG CACTCAGTGG 
AGTGGGACGG GTGGCCCGGC ACCACAGCCA CTTGGCCAGT TCCAGCCTGT GGGAACTGCC 
TCCTTACAGA ATTTCAACAT CAGCAATTTG CAGAAATCCA TCAGCAACCC CCCAGGTTCC 
AACCTGCGGA CCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_014108334.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963809.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X6, mRNA.

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CloneID	OCe43579
Clone ID Related Accession (Same CDS sequence)	XM_014108334.1 , XM_014108334.2
Accession Version	XM_014108334.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase WNK1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014108334.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG 
CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT 
CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC 
CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA 
GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG 
GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT 
CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA 
CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT 
CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA 
GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA 
TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG 
CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG 
TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT 
CTGGAGAGCA CTCAGGGAGT CTCTCAAGTT GCTCCTCCAG AGCCAGTTCC AGTGGCACAG 
GCACAGCCTA CCACTTTGGT CTCCTCTATA GACAGTGCAC ATTCAGATGT TGCTTCAGGA 
ATGAGTGATG GCAATGAGAA TGTCCCGTCC TCCAGTGGAA GGCATGAAGG GAGAGCTACA 
AAACGACATT ACCGAAAATC TGTAAGAAGT CGCTCTCGTC ATGAAAAGAC TTCGCGTCCA 
AAATTAAGAA TTTTGAATGT TTCAAATAAA GGAGACCGAG TAGTAGAATG TCAACTAGAG 
ACTCATAATC GGAAAATGGT TACATTCAAA TTTGATTTAG ATGGTGACAA TCCTGAGGAG 
ATAGCAACAA TTATGGTGAA CAATGACTTT ATTCTAGCAA TGGAGAGAGA GTCCTTTGTG 
GATCAAGTAC GAGAAATTAT TGAAAAGGCT GATGAAATGC TCAGTGAGGA TGTCAGTGTG 
GAACCAGAGG GTGACCAGGG ATTGGAGAGT CTGCAGGGAA AGGATGACTA TGGCTTTACA 
GGTTCTCAAA AATTGGAAGG AGAATTCAAA CAACCAGTTC CTGCGTCTTC CATGCCACAG 
CAAATAGGCA TTCCTACCAG TTCTTTAACT CAAGTTGTTC ATTCTGCTGG AAGACGGTTT 
ATAGTGAGTC CTGTGCCAGA AAGTCGATTA CGAGAATCAA AAGTTTTCAC TAGTGAAATA 
CCAGATACAG TTGCTGCCTC TACTCACAGC GCTGGAATGA ACTTGTCTCA CTCTGCTTCA 
TCCCTTAGCC TACAGCAAGC CTTTTCTGAA CTTAGACATG TGCAGATGAC AGAAGGGCCC 
AGCACAGCAC CTCCCAACTT CAGTCATACA GGACCAACAT TTCCAGTTGT TCCTCCTTCC 
ATGAGTAGCA TTGCTGGAGT CCCAACCACA GCAGTAGCCA CACCTTCAGT ATCAGTCCCT 
GCAACTAGCA GCCCTCTAAA TGACAGTTCC ACATCAGTAA TTCAATCTGA GATTCCAGTG 
CCCACTGAAA CAGGGGTTGG TGGAGTTCCC ACCTGCACAA GTGTAATGCC TTCAAGTGGT 
CTCCCTGTAC CACCCATATC TGAATCGCCA ATATTTTCCA CTATGGTTTC AAGCATCACA 
GTACCTACAG TTGTCTCAAT ATCAGCAACA TCCCAGCCAG TTCAAGCTCC CACATCTGGG 
AGCACTGTTT CTAGCATAGG AACTTTGCCT TCTATACCAG TTTCAACAGC TTTAGCCTCT 
GCAGTGGGCA GTGTTGCTAT CCCAGGTGCT AAGCCTCCAC CTGTACTATC ACAGCAGGCA 
GCAGGCAGTA CTACTGGGGC AGCCACATTA ACATCCGTTC CTGCCATCAC TCCGTTCCCA 
AGCATAGCTT CACAGCCATC TCTTCAGCTT AGCAGCAGCA CCTCTGCTCC AACTCTAGCT 
GAAACAGTGG TGGTTAGTGC ACACTCACTA GATAAAACAT CTCATAGCAG TACAACTGGA 
TTGGCTTTGT CCCTGTCTGC ATCATCTTCC TCCCCTGGAG CAGGATTATC TAGTTCTATT 
TCTCAGCCTG GTGGGGTGCA TCCTTTGGTT ACCCCATCAG CTATGGCTTC TATTCCTGCC 
CTTCCTCAAA CAGCAGGACC TACTTCTACA CCTTTATTGC CCCAAGTACT TAGTATCCCA 
CCCTTGGTAC AACCTGTTGC CAGTGTGCCT GCTGTGCAGC AGACACTAAT TCATAGTCAG 
CCTCAACCAG CTTTACTTCC CAACCAGCCC CACACTCACT GTCCTGAAAT AGATGCTGAC 
TTACAGCCCA AAGCTCCTGG AATTGATGAC ATAAAGACTC TAGAGGAAAA GCTGCGGTCT 
CTCTTTAGTG AACCGAGCTC ATCTGGAGCC CAACATACAT CGGTCTCACT AGAGACTTCA 
CTGGTAGTAG AGACCACTGT CACGCCAGGT ATCCCAACCA CTGCTATTGC ACCAAGCAAG 
CTCATGACAT CTACTACAAG TACATGCTTA CCACCAACCA GTTTGCAATT AGGAACATCT 
GGTTTGTCAG TTATACCAGT GGTCACGCCT GGGCAGGTTT CTACCCCAGT CAGCTATGTG 
TCAGCCCCAG GCAGCACTAC ATCAGGAGTG AAAGCTGGAA CTGCTCCATC AAAGCCACCT 
TTGACTAAAG CTCCAGTGCT GCCAGTGGGT ACTGAACTTC CAGCTGGTAC TCCACCCAGC 
GAGCAGCTGC CACCTTTTCC AGGACCTTCA CTAACTCAGT CCCAGCAACC TCTGGAAGAT 
CTTGATGCTC AGTTGAGAAG AACACTTAGT CCAGAGACTA TTGCGGTGAC ATCTACAATT 
GGTCCTGTGT CCATGGTGGC TCCAACAGCA GTCACAGAAG CAGGAGCACA GCCTCAGAAG 
GATGTTTCTC AGATCACAGA AGGTCCTGTC CTAGCGACTA GTTCGGGAAC TGGTGTTTTT 
AAGATGGGAA GATTTCAGGT TTCAGTTGCA ATGGATGATG TTCAAAAGGA GGGTAAAAAT 
AAGTTAGAAG ATGGAAAGTC TGTTCATTTT GAATCCAGCA CTTCAGAGTC CTCAGTGCTA 
TCAAGTAGTA GTCCAGAAAG TACCCTGGTG AAGACAGAGC CTAATGGGAT AACCATCCCT 
GGCATCTCAT CAGATATGCC AGATAGTGCC CACAAAACTC CTGCCTCAGA AGCAAAGTCA 
GAAACTGGGC AGCCTACCAA GGTTGGACGT TTTCAGGTGA CAACTACAGC AAACAAAGTA 
GGTCGTTTCT CTGTGTCAAG AACTGAAGAC AAGCTCATTG AGGCAAAGAA AGAGGGACCA 
ATAGCGTCTC CTCCTTTTAT GGATTTGGAA CAAGGTGTTC TTCCAGCTGT GATACCAAAG 
AAAGAAAAGC CTGAACTGTC AGAGCCTTCA CATCTGAACG GACCATCTTC TGACCTGGAG 
GCTGCCTTCC TGAGCAGGGA TGTGGATGAT GGTTCAGGTA GTCCACACTC CCCCCATCAG 
CTGAGCTCAA AGAGCCTGCC TATCCAGAAT CTAAGTCAAA GCCTTAGTAA TTCATTCAAC 
TCCTCTTACA TGAGTAGTGA CAATGAGTCA GATATTGAAG ATGAAGATTT AAAATTAGAG 
CTGCGAAGAC TACGAGAAAA ACATCTTAAA GAGATTCAAG ACCTGCAGAG TCGCCAGAAG 
CATGAAATTG AATCTTTGTA TACCAAACTG GGAAAGGTTC CCCCTGCTGT CATTATCCCC 
CCAGCTGCCC CCCTTTCAGG GAGAAGAAGG CGACCCACTA AAAGCAAAGG CAGCAAGTCT 
AGTCGCAGCA GTTCCTTGGG GAATAAAAGT CCCCAGCTTT CAGGTAACCT GTCTGGTCAG 
AGTGCTGCTT CAGTTTTGCA CGCTCAGCAG ACCCTCCACC CTCCTGGCAA CATCCCTGAG 
ACTGGGCCAA ATCAGCTGTT ACAGCCCCTT AAGCCATCTC CCTCCAGTGA CAACCTCTAC 
TCAGCCTTTA CCAGTGATGG TGCCATTTCA GTCCCAAGCC TTTCTGCTCC AGGTCAAGGC 
TGTGCGAAGT TTAACTGTGC ATCTGAGCAG GTGACATTCA AACCTGGTGG CAGGAGGACC 
CGATTTCTGA GGAAGATGGT GAAAAAAGTC TGTCCTTGCA ACCAGCTCTG TAGGACCAGC 
AGCACAAACA CTGTCGGGGG AACAGTGAAC AGCCAAGCTG CCCCAGCTCA GCCTCCTGCC 
ATGACATCCA GCAGGAAGGG CACATTCACA GATGACCTAC ACAAGTTGGT AGACAATTGG 
GCCCGAGATG CCATGAATCT CTCAGGCAGA AGAGGAAGCA AAGGACACAT GAATTATGAG 
GGCCCTGGAA TGGCAAGAAA GTTCTCCGCA CCTGGTCAGC TGTGCATCTC CATGACCTCA 
AATCTGGGTG GTTCTGCCCC CATCTCTGCA GCATCAGCTA CCTCTCTAGG TCACTTCACC 
AAGTCTATGT GCCCCCCACA GCAGTACGGT TTTCCAGCTC CCCCCTTTGG CACTCAGTGG 
AGTGGGACGG GTGGCCCGGC ACCACAGCCA CTTGGCCAGT TCCAGCCTGT GGGAACTGCC 
TCCTTACAGA ATTTCAACAT CAGCAATTTG CAGAAATCCA TCAGCAACCC CCCAGGTTCC 
AACCTGCGGA CCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963809.1
CDS	996..8273
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVGQSQGQPSSSSLTGIPSSLPIQHSQQQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQLPVSQPVPTIQGEPQIPVAAQPSVVPVHSGAHFLPVGQPLSTSLLPQYPVSQIPISTPHASAPQTGFSSLPITMAAGINQPLLTLASSATAAAIPGGPTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPSRLPPQYPGDSNIAPSSSVASICIPSTVLSPPMPTEALATPGYFPTVVQPFVESNLLVPVGSIGGQVQVSQPAVSLAQQAPTTSSQQAALESTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGCAKFNCASEQVTFKPGGRRTRFLRKMVKKVCPCNQLCRTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_014108334.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108334.2

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG 
CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT 
CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC 
CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA 
GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG 
GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT 
CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA 
CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT 
CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA 
GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA 
TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG 
CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG 
TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT 
CTGGAGAGCA CTCAGGGAGT CTCTCAAGTT GCTCCTCCAG AGCCAGTTCC AGTGGCACAG 
GCACAGCCTA CCACTTTGGT CTCCTCTATA GACAGTGCAC ATTCAGATGT TGCTTCAGGA 
ATGAGTGATG GCAATGAGAA TGTCCCGTCC TCCAGTGGAA GGCATGAAGG GAGAGCTACA 
AAACGACATT ACCGAAAATC TGTAAGAAGT CGCTCTCGTC ATGAAAAGAC TTCGCGTCCA 
AAATTAAGAA TTTTGAATGT TTCAAATAAA GGAGACCGAG TAGTAGAATG TCAACTAGAG 
ACTCATAATC GGAAAATGGT TACATTCAAA TTTGATTTAG ATGGTGACAA TCCTGAGGAG 
ATAGCAACAA TTATGGTGAA CAATGACTTT ATTCTAGCAA TGGAGAGAGA GTCCTTTGTG 
GATCAAGTAC GAGAAATTAT TGAAAAGGCT GATGAAATGC TCAGTGAGGA TGTCAGTGTG 
GAACCAGAGG GTGACCAGGG ATTGGAGAGT CTGCAGGGAA AGGATGACTA TGGCTTTACA 
GGTTCTCAAA AATTGGAAGG AGAATTCAAA CAACCAGTTC CTGCGTCTTC CATGCCACAG 
CAAATAGGCA TTCCTACCAG TTCTTTAACT CAAGTTGTTC ATTCTGCTGG AAGACGGTTT 
ATAGTGAGTC CTGTGCCAGA AAGTCGATTA CGAGAATCAA AAGTTTTCAC TAGTGAAATA 
CCAGATACAG TTGCTGCCTC TACTCACAGC GCTGGAATGA ACTTGTCTCA CTCTGCTTCA 
TCCCTTAGCC TACAGCAAGC CTTTTCTGAA CTTAGACATG TGCAGATGAC AGAAGGGCCC 
AGCACAGCAC CTCCCAACTT CAGTCATACA GGACCAACAT TTCCAGTTGT TCCTCCTTCC 
ATGAGTAGCA TTGCTGGAGT CCCAACCACA GCAGTAGCCA CACCTTCAGT ATCAGTCCCT 
GCAACTAGCA GCCCTCTAAA TGACAGTTCC ACATCAGTAA TTCAATCTGA GATTCCAGTG 
CCCACTGAAA CAGGGGTTGG TGGAGTTCCC ACCTGCACAA GTGTAATGCC TTCAAGTGGT 
CTCCCTGTAC CACCCATATC TGAATCGCCA ATATTTTCCA CTATGGTTTC AAGCATCACA 
GTACCTACAG TTGTCTCAAT ATCAGCAACA TCCCAGCCAG TTCAAGCTCC CACATCTGGG 
AGCACTGTTT CTAGCATAGG AACTTTGCCT TCTATACCAG TTTCAACAGC TTTAGCCTCT 
GCAGTGGGCA GTGTTGCTAT CCCAGGTGCT AAGCCTCCAC CTGTACTATC ACAGCAGGCA 
GCAGGCAGTA CTACTGGGGC AGCCACATTA ACATCCGTTC CTGCCATCAC TCCGTTCCCA 
AGCATAGCTT CACAGCCATC TCTTCAGCTT AGCAGCAGCA CCTCTGCTCC AACTCTAGCT 
GAAACAGTGG TGGTTAGTGC ACACTCACTA GATAAAACAT CTCATAGCAG TACAACTGGA 
TTGGCTTTGT CCCTGTCTGC ATCATCTTCC TCCCCTGGAG CAGGATTATC TAGTTCTATT 
TCTCAGCCTG GTGGGGTGCA TCCTTTGGTT ACCCCATCAG CTATGGCTTC TATTCCTGCC 
CTTCCTCAAA CAGCAGGACC TACTTCTACA CCTTTATTGC CCCAAGTACT TAGTATCCCA 
CCCTTGGTAC AACCTGTTGC CAGTGTGCCT GCTGTGCAGC AGACACTAAT TCATAGTCAG 
CCTCAACCAG CTTTACTTCC CAACCAGCCC CACACTCACT GTCCTGAAAT AGATGCTGAC 
TTACAGCCCA AAGCTCCTGG AATTGATGAC ATAAAGACTC TAGAGGAAAA GCTGCGGTCT 
CTCTTTAGTG AACCGAGCTC ATCTGGAGCC CAACATACAT CGGTCTCACT AGAGACTTCA 
CTGGTAGTAG AGACCACTGT CACGCCAGGT ATCCCAACCA CTGCTATTGC ACCAAGCAAG 
CTCATGACAT CTACTACAAG TACATGCTTA CCACCAACCA GTTTGCAATT AGGAACATCT 
GGTTTGTCAG TTATACCAGT GGTCACGCCT GGGCAGGTTT CTACCCCAGT CAGCTATGTG 
TCAGCCCCAG GCAGCACTAC ATCAGGAGTG AAAGCTGGAA CTGCTCCATC AAAGCCACCT 
TTGACTAAAG CTCCAGTGCT GCCAGTGGGT ACTGAACTTC CAGCTGGTAC TCCACCCAGC 
GAGCAGCTGC CACCTTTTCC AGGACCTTCA CTAACTCAGT CCCAGCAACC TCTGGAAGAT 
CTTGATGCTC AGTTGAGAAG AACACTTAGT CCAGAGACTA TTGCGGTGAC ATCTACAATT 
GGTCCTGTGT CCATGGTGGC TCCAACAGCA GTCACAGAAG CAGGAGCACA GCCTCAGAAG 
GATGTTTCTC AGATCACAGA AGGTCCTGTC CTAGCGACTA GTTCGGGAAC TGGTGTTTTT 
AAGATGGGAA GATTTCAGGT TTCAGTTGCA ATGGATGATG TTCAAAAGGA GGGTAAAAAT 
AAGTTAGAAG ATGGAAAGTC TGTTCATTTT GAATCCAGCA CTTCAGAGTC CTCAGTGCTA 
TCAAGTAGTA GTCCAGAAAG TACCCTGGTG AAGACAGAGC CTAATGGGAT AACCATCCCT 
GGCATCTCAT CAGATATGCC AGATAGTGCC CACAAAACTC CTGCCTCAGA AGCAAAGTCA 
GAAACTGGGC AGCCTACCAA GGTTGGACGT TTTCAGGTGA CAACTACAGC AAACAAAGTA 
GGTCGTTTCT CTGTGTCAAG AACTGAAGAC AAGCTCATTG AGGCAAAGAA AGAGGGACCA 
ATAGCGTCTC CTCCTTTTAT GGATTTGGAA CAAGGTGTTC TTCCAGCTGT GATACCAAAG 
AAAGAAAAGC CTGAACTGTC AGAGCCTTCA CATCTGAACG GACCATCTTC TGACCTGGAG 
GCTGCCTTCC TGAGCAGGGA TGTGGATGAT GGTTCAGGTA GTCCACACTC CCCCCATCAG 
CTGAGCTCAA AGAGCCTGCC TATCCAGAAT CTAAGTCAAA GCCTTAGTAA TTCATTCAAC 
TCCTCTTACA TGAGTAGTGA CAATGAGTCA GATATTGAAG ATGAAGATTT AAAATTAGAG 
CTGCGAAGAC TACGAGAAAA ACATCTTAAA GAGATTCAAG ACCTGCAGAG TCGCCAGAAG 
CATGAAATTG AATCTTTGTA TACCAAACTG GGAAAGGTTC CCCCTGCTGT CATTATCCCC 
CCAGCTGCCC CCCTTTCAGG GAGAAGAAGG CGACCCACTA AAAGCAAAGG CAGCAAGTCT 
AGTCGCAGCA GTTCCTTGGG GAATAAAAGT CCCCAGCTTT CAGGTAACCT GTCTGGTCAG 
AGTGCTGCTT CAGTTTTGCA CGCTCAGCAG ACCCTCCACC CTCCTGGCAA CATCCCTGAG 
ACTGGGCCAA ATCAGCTGTT ACAGCCCCTT AAGCCATCTC CCTCCAGTGA CAACCTCTAC 
TCAGCCTTTA CCAGTGATGG TGCCATTTCA GTCCCAAGCC TTTCTGCTCC AGGTCAAGGC 
TGTGCGAAGT TTAACTGTGC ATCTGAGCAG GTGACATTCA AACCTGGTGG CAGGAGGACC 
CGATTTCTGA GGAAGATGGT GAAAAAAGTC TGTCCTTGCA ACCAGCTCTG TAGGACCAGC 
AGCACAAACA CTGTCGGGGG AACAGTGAAC AGCCAAGCTG CCCCAGCTCA GCCTCCTGCC 
ATGACATCCA GCAGGAAGGG CACATTCACA GATGACCTAC ACAAGTTGGT AGACAATTGG 
GCCCGAGATG CCATGAATCT CTCAGGCAGA AGAGGAAGCA AAGGACACAT GAATTATGAG 
GGCCCTGGAA TGGCAAGAAA GTTCTCCGCA CCTGGTCAGC TGTGCATCTC CATGACCTCA 
AATCTGGGTG GTTCTGCCCC CATCTCTGCA GCATCAGCTA CCTCTCTAGG TCACTTCACC 
AAGTCTATGT GCCCCCCACA GCAGTACGGT TTTCCAGCTC CCCCCTTTGG CACTCAGTGG 
AGTGGGACGG GTGGCCCGGC ACCACAGCCA CTTGGCCAGT TCCAGCCTGT GGGAACTGCC 
TCCTTACAGA ATTTCAACAT CAGCAATTTG CAGAAATCCA TCAGCAACCC CCCAGGTTCC 
AACCTGCGGA CCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_014108335.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963810.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X12, mRNA.

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CloneID	OCe43580
Clone ID Related Accession (Same CDS sequence)	XM_014108335.1
Accession Version	XM_014108335.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase WNK1 isoform X12
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG 
CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT 
CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC 
CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA 
GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG 
GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT 
CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA 
CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT 
CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA 
GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA 
TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG 
CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG 
TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT 
CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA 
AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG 
CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT 
GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA 
CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT 
AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA 
GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT 
CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG 
AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT 
ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT 
AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA 
GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT 
AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT 
AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT 
GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT 
GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT 
ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT 
GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG 
GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC 
AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA 
GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA 
GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT 
TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG 
CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT 
CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG 
GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG 
CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT 
AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA 
GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG 
ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG 
TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC 
CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT 
AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG 
CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT 
GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT 
GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT 
TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG 
GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA 
GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT 
AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC 
TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT 
GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT 
TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG 
TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA 
AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC 
TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC 
TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT 
TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA 
AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA 
ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT 
GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC 
AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT 
GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG 
CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC 
TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGGAAGATG 
GTGAAAAAAG TCTGTCCTTG CAACCAGCTC TGTAGGACCA GCAGCACAAA CACTGTCGGG 
GGAACAGTGA ACAGCCAAGC TGCCCCAGCT CAGCCTCCTG CCATGACATC CAGCAGGAAG 
GGCACATTCA CAGATGACCT ACACAAGTTG GTAGACAATT GGGCCCGAGA TGCCATGAAT 
CTCTCAGGCA GAAGAGGAAG CAAAGGACAC ATGAATTATG AGGGCCCTGG AATGGCAAGA 
AAGTTCTCCG CACCTGGTCA GCTGTGCATC TCCATGACCT CAAATCTGGG TGGTTCTGCC 
CCCATCTCTG CAGCATCAGC TACCTCTCTA GGTCACTTCA CCAAGTCTAT GTGCCCCCCA 
CAGCAGTACG GTTTTCCAGC TCCCCCCTTT GGCACTCAGT GGAGTGGGAC GGGTGGCCCG 
GCACCACAGC CACTTGGCCA GTTCCAGCCT GTGGGAACTG CCTCCTTACA GAATTTCAAC 
ATCAGCAATT TGCAGAAATC CATCAGCAAC CCCCCAGGTT CCAACCTGCG GACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963810.1
CDS	99..7358
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVGQSQGQPSSSSLTGIPSSLPIQHSQQQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQLPVSQPVPTIQGEPQIPVAAQPSVVPVHSGAHFLPVGQPLSTSLLPQYPVSQIPISTPHASAPQTGFSSLPITMAAGINQPLLTLASSATAAAIPGGPTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPSRLPPQYPGDSNIAPSSSVASICIPSTVLSPPMPTEALATPGYFPTVVQPFVESNLLVPVGSIGGQVQVSQPAVSLAQQAPTTSSQQAALEQNEMPMWGPCCHWLFCWESTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGKMVKKVCPCNQLCRTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_014108335.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108335.1

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG 
CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT 
CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC 
CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA 
GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG 
GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT 
CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA 
CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT 
CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA 
GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA 
TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG 
CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG 
TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT 
CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA 
AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG 
CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT 
GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA 
CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT 
AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA 
GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT 
CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG 
AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT 
ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT 
AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA 
GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT 
AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT 
AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT 
GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT 
GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT 
ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT 
GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG 
GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC 
AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA 
GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA 
GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT 
TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG 
CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT 
CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG 
GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG 
CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT 
AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA 
GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG 
ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG 
TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC 
CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT 
AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG 
CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT 
GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT 
GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT 
TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG 
GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA 
GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT 
AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC 
TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT 
GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT 
TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG 
TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA 
AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC 
TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC 
TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT 
TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA 
AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA 
ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT 
GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC 
AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT 
GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG 
CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC 
TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGGAAGATG 
GTGAAAAAAG TCTGTCCTTG CAACCAGCTC TGTAGGACCA GCAGCACAAA CACTGTCGGG 
GGAACAGTGA ACAGCCAAGC TGCCCCAGCT CAGCCTCCTG CCATGACATC CAGCAGGAAG 
GGCACATTCA CAGATGACCT ACACAAGTTG GTAGACAATT GGGCCCGAGA TGCCATGAAT 
CTCTCAGGCA GAAGAGGAAG CAAAGGACAC ATGAATTATG AGGGCCCTGG AATGGCAAGA 
AAGTTCTCCG CACCTGGTCA GCTGTGCATC TCCATGACCT CAAATCTGGG TGGTTCTGCC 
CCCATCTCTG CAGCATCAGC TACCTCTCTA GGTCACTTCA CCAAGTCTAT GTGCCCCCCA 
CAGCAGTACG GTTTTCCAGC TCCCCCCTTT GGCACTCAGT GGAGTGGGAC GGGTGGCCCG 
GCACCACAGC CACTTGGCCA GTTCCAGCCT GTGGGAACTG CCTCCTTACA GAATTTCAAC 
ATCAGCAATT TGCAGAAATC CATCAGCAAC CCCCCAGGTT CCAACCTGCG GACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_014108336.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963811.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X10, mRNA.

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CloneID	OCe43581
Clone ID Related Accession (Same CDS sequence)	XM_014108336.2 , XM_014108336.1
Accession Version	XM_014108336.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7218bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase WNK1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014108336.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG 
CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT 
CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC 
CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA 
GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG 
GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT 
CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA 
CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT 
CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA 
GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA 
TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG 
CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG 
TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT 
CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA 
AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG 
CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT 
GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA 
CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT 
AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA 
GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT 
CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG 
AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT 
ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT 
AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA 
GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT 
AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT 
AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT 
GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT 
GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT 
ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT 
GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG 
GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC 
AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA 
GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA 
GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT 
TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG 
CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT 
CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG 
GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG 
CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT 
AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA 
GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG 
ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG 
TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC 
CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT 
AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG 
CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT 
GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT 
GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT 
TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG 
GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA 
GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT 
AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC 
TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT 
GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT 
TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG 
TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA 
AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC 
TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC 
TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT 
TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA 
AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA 
ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT 
GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC 
AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT 
GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG 
CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC 
TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGGACCAGC 
AGCACAAACA CTGTCGGGGG AACAGTGAAC AGCCAAGCTG CCCCAGCTCA GCCTCCTGCC 
ATGACATCCA GCAGGAAGGG CACATTCACA GATGACCTAC ACAAGTTGGT AGACAATTGG 
GCCCGAGATG CCATGAATCT CTCAGGCAGA AGAGGAAGCA AAGGACACAT GAATTATGAG 
GGCCCTGGAA TGGCAAGAAA GTTCTCCGCA CCTGGTCAGC TGTGCATCTC CATGACCTCA 
AATCTGGGTG GTTCTGCCCC CATCTCTGCA GCATCAGCTA CCTCTCTAGG TCACTTCACC 
AAGTCTATGT GCCCCCCACA GCAGTACGGT TTTCCAGCTC CCCCCTTTGG CACTCAGTGG 
AGTGGGACGG GTGGCCCGGC ACCACAGCCA CTTGGCCAGT TCCAGCCTGT GGGAACTGCC 
TCCTTACAGA ATTTCAACAT CAGCAATTTG CAGAAATCCA TCAGCAACCC CCCAGGTTCC 
AACCTGCGGA CCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963811.1
CDS	995..8212
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVGQSQGQPSSSSLTGIPSSLPIQHSQQQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQLPVSQPVPTIQGEPQIPVAAQPSVVPVHSGAHFLPVGQPLSTSLLPQYPVSQIPISTPHASAPQTGFSSLPITMAAGINQPLLTLASSATAAAIPGGPTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPSRLPPQYPGDSNIAPSSSVASICIPSTVLSPPMPTEALATPGYFPTVVQPFVESNLLVPVGSIGGQVQVSQPAVSLAQQAPTTSSQQAALEQNEMPMWGPCCHWLFCWESTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_014108336.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108336.2

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG 
CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT 
CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC 
CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA 
GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG 
GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT 
CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA 
CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT 
CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA 
GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA 
TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG 
CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG 
TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT 
CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA 
AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG 
CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT 
GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA 
CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT 
AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA 
GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT 
CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG 
AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT 
ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT 
AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA 
GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT 
AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT 
AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT 
GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT 
GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT 
ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT 
GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG 
GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC 
AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA 
GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA 
GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT 
TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG 
CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT 
CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG 
GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG 
CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT 
AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA 
GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG 
ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG 
TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC 
CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT 
AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG 
CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT 
GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT 
GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT 
TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG 
GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA 
GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT 
AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC 
TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT 
GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT 
TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG 
TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA 
AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC 
TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC 
TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT 
TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA 
AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA 
ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT 
GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC 
AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT 
GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG 
CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC 
TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGGACCAGC 
AGCACAAACA CTGTCGGGGG AACAGTGAAC AGCCAAGCTG CCCCAGCTCA GCCTCCTGCC 
ATGACATCCA GCAGGAAGGG CACATTCACA GATGACCTAC ACAAGTTGGT AGACAATTGG 
GCCCGAGATG CCATGAATCT CTCAGGCAGA AGAGGAAGCA AAGGACACAT GAATTATGAG 
GGCCCTGGAA TGGCAAGAAA GTTCTCCGCA CCTGGTCAGC TGTGCATCTC CATGACCTCA 
AATCTGGGTG GTTCTGCCCC CATCTCTGCA GCATCAGCTA CCTCTCTAGG TCACTTCACC 
AAGTCTATGT GCCCCCCACA GCAGTACGGT TTTCCAGCTC CCCCCTTTGG CACTCAGTGG 
AGTGGGACGG GTGGCCCGGC ACCACAGCCA CTTGGCCAGT TCCAGCCTGT GGGAACTGCC 
TCCTTACAGA ATTTCAACAT CAGCAATTTG CAGAAATCCA TCAGCAACCC CCCAGGTTCC 
AACCTGCGGA CCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_014108336.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963811.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X13, mRNA.

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CloneID	OCe43581
Clone ID Related Accession (Same CDS sequence)	XM_014108336.2 , XM_014108336.1
Accession Version	XM_014108336.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7218bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase WNK1 isoform X13
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG 
CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT 
CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC 
CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA 
GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG 
GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT 
CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA 
CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT 
CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA 
GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA 
TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG 
CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG 
TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT 
CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA 
AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG 
CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT 
GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA 
CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT 
AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA 
GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT 
CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG 
AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT 
ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT 
AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA 
GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT 
AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT 
AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT 
GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT 
GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT 
ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT 
GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG 
GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC 
AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA 
GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA 
GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT 
TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG 
CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT 
CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG 
GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG 
CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT 
AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA 
GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG 
ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG 
TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC 
CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT 
AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG 
CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT 
GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT 
GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT 
TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG 
GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA 
GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT 
AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC 
TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT 
GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT 
TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG 
TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA 
AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC 
TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC 
TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT 
TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA 
AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA 
ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT 
GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC 
AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT 
GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG 
CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC 
TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGGACCAGC 
AGCACAAACA CTGTCGGGGG AACAGTGAAC AGCCAAGCTG CCCCAGCTCA GCCTCCTGCC 
ATGACATCCA GCAGGAAGGG CACATTCACA GATGACCTAC ACAAGTTGGT AGACAATTGG 
GCCCGAGATG CCATGAATCT CTCAGGCAGA AGAGGAAGCA AAGGACACAT GAATTATGAG 
GGCCCTGGAA TGGCAAGAAA GTTCTCCGCA CCTGGTCAGC TGTGCATCTC CATGACCTCA 
AATCTGGGTG GTTCTGCCCC CATCTCTGCA GCATCAGCTA CCTCTCTAGG TCACTTCACC 
AAGTCTATGT GCCCCCCACA GCAGTACGGT TTTCCAGCTC CCCCCTTTGG CACTCAGTGG 
AGTGGGACGG GTGGCCCGGC ACCACAGCCA CTTGGCCAGT TCCAGCCTGT GGGAACTGCC 
TCCTTACAGA ATTTCAACAT CAGCAATTTG CAGAAATCCA TCAGCAACCC CCCAGGTTCC 
AACCTGCGGA CCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963811.1
CDS	99..7316
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVGQSQGQPSSSSLTGIPSSLPIQHSQQQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQLPVSQPVPTIQGEPQIPVAAQPSVVPVHSGAHFLPVGQPLSTSLLPQYPVSQIPISTPHASAPQTGFSSLPITMAAGINQPLLTLASSATAAAIPGGPTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPSRLPPQYPGDSNIAPSSSVASICIPSTVLSPPMPTEALATPGYFPTVVQPFVESNLLVPVGSIGGQVQVSQPAVSLAQQAPTTSSQQAALEQNEMPMWGPCCHWLFCWESTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_014108336.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108336.1

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG 
CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT 
CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC 
CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA 
GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG 
GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT 
CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA 
CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT 
CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA 
GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA 
TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG 
CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG 
TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT 
CTGGAGCAGA ATGAAATGCC AATGTGGGGG CCTTGTTGCC ACTGGCTGTT CTGTTGGGAA 
AGCACTCAGG GAGTCTCTCA AGTTGCTCCT CCAGAGCCAG TTCCAGTGGC ACAGGCACAG 
CCTACCACTT TGGTCTCCTC TATAGACAGT GCACATTCAG ATGTTGCTTC AGGAATGAGT 
GATGGCAATG AGAATGTCCC GTCCTCCAGT GGAAGGCATG AAGGGAGAGC TACAAAACGA 
CATTACCGAA AATCTGTAAG AAGTCGCTCT CGTCATGAAA AGACTTCGCG TCCAAAATTA 
AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAACT AGAGACTCAT 
AATCGGAAAA TGGTTACATT CAAATTTGAT TTAGATGGTG ACAATCCTGA GGAGATAGCA 
ACAATTATGG TGAACAATGA CTTTATTCTA GCAATGGAGA GAGAGTCCTT TGTGGATCAA 
GTACGAGAAA TTATTGAAAA GGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA 
GAGGGTGACC AGGGATTGGA GAGTCTGCAG GGAAAGGATG ACTATGGCTT TACAGGTTCT 
CAAAAATTGG AAGGAGAATT CAAACAACCA GTTCCTGCGT CTTCCATGCC ACAGCAAATA 
GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CTGGAAGACG GTTTATAGTG 
AGTCCTGTGC CAGAAAGTCG ATTACGAGAA TCAAAAGTTT TCACTAGTGA AATACCAGAT 
ACAGTTGCTG CCTCTACTCA CAGCGCTGGA ATGAACTTGT CTCACTCTGC TTCATCCCTT 
AGCCTACAGC AAGCCTTTTC TGAACTTAGA CATGTGCAGA TGACAGAAGG GCCCAGCACA 
GCACCTCCCA ACTTCAGTCA TACAGGACCA ACATTTCCAG TTGTTCCTCC TTCCATGAGT 
AGCATTGCTG GAGTCCCAAC CACAGCAGTA GCCACACCTT CAGTATCAGT CCCTGCAACT 
AGCAGCCCTC TAAATGACAG TTCCACATCA GTAATTCAAT CTGAGATTCC AGTGCCCACT 
GAAACAGGGG TTGGTGGAGT TCCCACCTGC ACAAGTGTAA TGCCTTCAAG TGGTCTCCCT 
GTACCACCCA TATCTGAATC GCCAATATTT TCCACTATGG TTTCAAGCAT CACAGTACCT 
ACAGTTGTCT CAATATCAGC AACATCCCAG CCAGTTCAAG CTCCCACATC TGGGAGCACT 
GTTTCTAGCA TAGGAACTTT GCCTTCTATA CCAGTTTCAA CAGCTTTAGC CTCTGCAGTG 
GGCAGTGTTG CTATCCCAGG TGCTAAGCCT CCACCTGTAC TATCACAGCA GGCAGCAGGC 
AGTACTACTG GGGCAGCCAC ATTAACATCC GTTCCTGCCA TCACTCCGTT CCCAAGCATA 
GCTTCACAGC CATCTCTTCA GCTTAGCAGC AGCACCTCTG CTCCAACTCT AGCTGAAACA 
GTGGTGGTTA GTGCACACTC ACTAGATAAA ACATCTCATA GCAGTACAAC TGGATTGGCT 
TTGTCCCTGT CTGCATCATC TTCCTCCCCT GGAGCAGGAT TATCTAGTTC TATTTCTCAG 
CCTGGTGGGG TGCATCCTTT GGTTACCCCA TCAGCTATGG CTTCTATTCC TGCCCTTCCT 
CAAACAGCAG GACCTACTTC TACACCTTTA TTGCCCCAAG TACTTAGTAT CCCACCCTTG 
GTACAACCTG TTGCCAGTGT GCCTGCTGTG CAGCAGACAC TAATTCATAG TCAGCCTCAA 
CCAGCTTTAC TTCCCAACCA GCCCCACACT CACTGTCCTG AAATAGATGC TGACTTACAG 
CCCAAAGCTC CTGGAATTGA TGACATAAAG ACTCTAGAGG AAAAGCTGCG GTCTCTCTTT 
AGTGAACCGA GCTCATCTGG AGCCCAACAT ACATCGGTCT CACTAGAGAC TTCACTGGTA 
GTAGAGACCA CTGTCACGCC AGGTATCCCA ACCACTGCTA TTGCACCAAG CAAGCTCATG 
ACATCTACTA CAAGTACATG CTTACCACCA ACCAGTTTGC AATTAGGAAC ATCTGGTTTG 
TCAGTTATAC CAGTGGTCAC GCCTGGGCAG GTTTCTACCC CAGTCAGCTA TGTGTCAGCC 
CCAGGCAGCA CTACATCAGG AGTGAAAGCT GGAACTGCTC CATCAAAGCC ACCTTTGACT 
AAAGCTCCAG TGCTGCCAGT GGGTACTGAA CTTCCAGCTG GTACTCCACC CAGCGAGCAG 
CTGCCACCTT TTCCAGGACC TTCACTAACT CAGTCCCAGC AACCTCTGGA AGATCTTGAT 
GCTCAGTTGA GAAGAACACT TAGTCCAGAG ACTATTGCGG TGACATCTAC AATTGGTCCT 
GTGTCCATGG TGGCTCCAAC AGCAGTCACA GAAGCAGGAG CACAGCCTCA GAAGGATGTT 
TCTCAGATCA CAGAAGGTCC TGTCCTAGCG ACTAGTTCGG GAACTGGTGT TTTTAAGATG 
GGAAGATTTC AGGTTTCAGT TGCAATGGAT GATGTTCAAA AGGAGGGTAA AAATAAGTTA 
GAAGATGGAA AGTCTGTTCA TTTTGAATCC AGCACTTCAG AGTCCTCAGT GCTATCAAGT 
AGTAGTCCAG AAAGTACCCT GGTGAAGACA GAGCCTAATG GGATAACCAT CCCTGGCATC 
TCATCAGATA TGCCAGATAG TGCCCACAAA ACTCCTGCCT CAGAAGCAAA GTCAGAAACT 
GGGCAGCCTA CCAAGGTTGG ACGTTTTCAG GTGACAACTA CAGCAAACAA AGTAGGTCGT 
TTCTCTGTGT CAAGAACTGA AGACAAGCTC ATTGAGGCAA AGAAAGAGGG ACCAATAGCG 
TCTCCTCCTT TTATGGATTT GGAACAAGGT GTTCTTCCAG CTGTGATACC AAAGAAAGAA 
AAGCCTGAAC TGTCAGAGCC TTCACATCTG AACGGACCAT CTTCTGACCT GGAGGCTGCC 
TTCCTGAGCA GGGATGTGGA TGATGGTTCA GGTAGTCCAC ACTCCCCCCA TCAGCTGAGC 
TCAAAGAGCC TGCCTATCCA GAATCTAAGT CAAAGCCTTA GTAATTCATT CAACTCCTCT 
TACATGAGTA GTGACAATGA GTCAGATATT GAAGATGAAG ATTTAAAATT AGAGCTGCGA 
AGACTACGAG AAAAACATCT TAAAGAGATT CAAGACCTGC AGAGTCGCCA GAAGCATGAA 
ATTGAATCTT TGTATACCAA ACTGGGAAAG GTTCCCCCTG CTGTCATTAT CCCCCCAGCT 
GCCCCCCTTT CAGGGAGAAG AAGGCGACCC ACTAAAAGCA AAGGCAGCAA GTCTAGTCGC 
AGCAGTTCCT TGGGGAATAA AAGTCCCCAG CTTTCAGGTA ACCTGTCTGG TCAGAGTGCT 
GCTTCAGTTT TGCACGCTCA GCAGACCCTC CACCCTCCTG GCAACATCCC TGAGACTGGG 
CCAAATCAGC TGTTACAGCC CCTTAAGCCA TCTCCCTCCA GTGACAACCT CTACTCAGCC 
TTTACCAGTG ATGGTGCCAT TTCAGTCCCA AGCCTTTCTG CTCCAGGTCA AGGGACCAGC 
AGCACAAACA CTGTCGGGGG AACAGTGAAC AGCCAAGCTG CCCCAGCTCA GCCTCCTGCC 
ATGACATCCA GCAGGAAGGG CACATTCACA GATGACCTAC ACAAGTTGGT AGACAATTGG 
GCCCGAGATG CCATGAATCT CTCAGGCAGA AGAGGAAGCA AAGGACACAT GAATTATGAG 
GGCCCTGGAA TGGCAAGAAA GTTCTCCGCA CCTGGTCAGC TGTGCATCTC CATGACCTCA 
AATCTGGGTG GTTCTGCCCC CATCTCTGCA GCATCAGCTA CCTCTCTAGG TCACTTCACC 
AAGTCTATGT GCCCCCCACA GCAGTACGGT TTTCCAGCTC CCCCCTTTGG CACTCAGTGG 
AGTGGGACGG GTGGCCCGGC ACCACAGCCA CTTGGCCAGT TCCAGCCTGT GGGAACTGCC 
TCCTTACAGA ATTTCAACAT CAGCAATTTG CAGAAATCCA TCAGCAACCC CCCAGGTTCC 
AACCTGCGGA CCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_014108338.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963813.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X14, mRNA.

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CloneID	OCe43582
Clone ID Related Accession (Same CDS sequence)	XM_014108338.1 , XM_014108338.2
Accession Version	XM_014108338.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase WNK1 isoform X14
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG 
CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT 
CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC 
CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA 
GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG 
GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT 
CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA 
CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT 
CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA 
GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA 
TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG 
CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG 
TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT 
CTGGAGAGCA CTCAGGGAGT CTCTCAAGTT GCTCCTCCAG AGCCAGTTCC AGTGGCACAG 
GCACAGCCTA CCACTTTGGT CTCCTCTATA GACAGTGCAC ATTCAGATGT TGCTTCAGGA 
ATGAGTGATG GCAATGAGAA TGTCCCGTCC TCCAGTGGAA GGCATGAAGG GAGAGCTACA 
AAACGACATT ACCGAAAATC TGTAAGAAGT CGCTCTCGTC ATGAAAAGAC TTCGCGTCCA 
AAATTAAGAA TTTTGAATGT TTCAAATAAA GGAGACCGAG TAGTAGAATG TCAACTAGAG 
ACTCATAATC GGAAAATGGT TACATTCAAA TTTGATTTAG ATGGTGACAA TCCTGAGGAG 
ATAGCAACAA TTATGGTGAA CAATGACTTT ATTCTAGCAA TGGAGAGAGA GTCCTTTGTG 
GATCAAGTAC GAGAAATTAT TGAAAAGGCT GATGAAATGC TCAGTGAGGA TGTCAGTGTG 
GAACCAGAGG GTGACCAGGG ATTGGAGAGT CTGCAGGGAA AGGATGACTA TGGCTTTACA 
GGTTCTCAAA AATTGGAAGG AGAATTCAAA CAACCAGTTC CTGCGTCTTC CATGCCACAG 
CAAATAGGCA TTCCTACCAG TTCTTTAACT CAAGTTGTTC ATTCTGCTGG AAGACGGTTT 
ATAGTGAGTC CTGTGCCAGA AAGTCGATTA CGAGAATCAA AAGTTTTCAC TAGTGAAATA 
CCAGATACAG TTGCTGCCTC TACTCACAGC GCTGGAATGA ACTTGTCTCA CTCTGCTTCA 
TCCCTTAGCC TACAGCAAGC CTTTTCTGAA CTTAGACATG TGCAGATGAC AGAAGGGCCC 
AGCACAGCAC CTCCCAACTT CAGTCATACA GGACCAACAT TTCCAGTTGT TCCTCCTTCC 
ATGAGTAGCA TTGCTGGAGT CCCAACCACA GCAGTAGCCA CACCTTCAGT ATCAGTCCCT 
GCAACTAGCA GCCCTCTAAA TGACAGTTCC ACATCAGTAA TTCAATCTGA GATTCCAGTG 
CCCACTGAAA CAGGGGTTGG TGGAGTTCCC ACCTGCACAA GTGTAATGCC TTCAAGTGGT 
CTCCCTGTAC CACCCATATC TGAATCGCCA ATATTTTCCA CTATGGTTTC AAGCATCACA 
GTACCTACAG TTGTCTCAAT ATCAGCAACA TCCCAGCCAG TTCAAGCTCC CACATCTGGG 
AGCACTGTTT CTAGCATAGG AACTTTGCCT TCTATACCAG TTTCAACAGC TTTAGCCTCT 
GCAGTGGGCA GTGTTGCTAT CCCAGGTGCT AAGCCTCCAC CTGTACTATC ACAGCAGGCA 
GCAGGCAGTA CTACTGGGGC AGCCACATTA ACATCCGTTC CTGCCATCAC TCCGTTCCCA 
AGCATAGCTT CACAGCCATC TCTTCAGCTT AGCAGCAGCA CCTCTGCTCC AACTCTAGCT 
GAAACAGTGG TGGTTAGTGC ACACTCACTA GATAAAACAT CTCATAGCAG TACAACTGGA 
TTGGCTTTGT CCCTGTCTGC ATCATCTTCC TCCCCTGGAG CAGGATTATC TAGTTCTATT 
TCTCAGCCTG GTGGGGTGCA TCCTTTGGTT ACCCCATCAG CTATGGCTTC TATTCCTGCC 
CTTCCTCAAA CAGCAGGACC TACTTCTACA CCTTTATTGC CCCAAGTACT TAGTATCCCA 
CCCTTGGTAC AACCTGTTGC CAGTGTGCCT GCTGTGCAGC AGACACTAAT TCATAGTCAG 
CCTCAACCAG CTTTACTTCC CAACCAGCCC CACACTCACT GTCCTGAAAT AGATGCTGAC 
TTACAGCCCA AAGCTCCTGG AATTGATGAC ATAAAGACTC TAGAGGAAAA GCTGCGGTCT 
CTCTTTAGTG AACCGAGCTC ATCTGGAGCC CAACATACAT CGGTCTCACT AGAGACTTCA 
CTGGTAGTAG AGACCACTGT CACGCCAGGT ATCCCAACCA CTGCTATTGC ACCAAGCAAG 
CTCATGACAT CTACTACAAG TACATGCTTA CCACCAACCA GTTTGCAATT AGGAACATCT 
GGTTTGTCAG TTATACCAGT GGTCACGCCT GGGCAGGTTT CTACCCCAGT CAGCTATGTG 
TCAGCCCCAG GCAGCACTAC ATCAGGAGTG AAAGCTGGAA CTGCTCCATC AAAGCCACCT 
TTGACTAAAG CTCCAGTGCT GCCAGTGGGT ACTGAACTTC CAGCTGGTAC TCCACCCAGC 
GAGCAGCTGC CACCTTTTCC AGGACCTTCA CTAACTCAGT CCCAGCAACC TCTGGAAGAT 
CTTGATGCTC AGTTGAGAAG AACACTTAGT CCAGAGACTA TTGCGGTGAC ATCTACAATT 
GGTCCTGTGT CCATGGTGGC TCCAACAGCA GTCACAGAAG CAGGAGCACA GCCTCAGAAG 
GATGTTTCTC AGATCACAGA AGGTCCTGTC CTAGCGACTA GTTCGGGAAC TGGTGTTTTT 
AAGATGGGAA GATTTCAGGT TTCAGTTGCA ATGGATGATG TTCAAAAGGA GGGTAAAAAT 
AAGTTAGAAG ATGGAAAGTC TGTTCATTTT GAATCCAGCA CTTCAGAGTC CTCAGTGCTA 
TCAAGTAGTA GTCCAGAAAG TACCCTGGTG AAGACAGAGC CTAATGGGAT AACCATCCCT 
GGCATCTCAT CAGATATGCC AGATAGTGCC CACAAAACTC CTGCCTCAGA AGCAAAGTCA 
GAAACTGGGC AGCCTACCAA GGTTGGACGT TTTCAGGTGA CAACTACAGC AAACAAAGTA 
GGTCGTTTCT CTGTGTCAAG AACTGAAGAC AAGCTCATTG AGGCAAAGAA AGAGGGACCA 
ATAGCGTCTC CTCCTTTTAT GGATTTGGAA CAAGGTGTTC TTCCAGCTGT GATACCAAAG 
AAAGAAAAGC CTGAACTGTC AGAGCCTTCA CATCTGAACG GACCATCTTC TGACCTGGAG 
GCTGCCTTCC TGAGCAGGGA TGTGGATGAT GGTTCAGGTA GTCCACACTC CCCCCATCAG 
CTGAGCTCAA AGAGCCTGCC TATCCAGAAT CTAAGTCAAA GCCTTAGTAA TTCATTCAAC 
TCCTCTTACA TGAGTAGTGA CAATGAGTCA GATATTGAAG ATGAAGATTT AAAATTAGAG 
CTGCGAAGAC TACGAGAAAA ACATCTTAAA GAGATTCAAG ACCTGCAGAG TCGCCAGAAG 
CATGAAATTG AATCTTTGTA TACCAAACTG GGAAAGGTTC CCCCTGCTGT CATTATCCCC 
CCAGCTGCCC CCCTTTCAGG GAGAAGAAGG CGACCCACTA AAAGCAAAGG CAGCAAGTCT 
AGTCGCAGCA GTTCCTTGGG GAATAAAAGT CCCCAGCTTT CAGGTAACCT GTCTGGTCAG 
AGTGCTGCTT CAGTTTTGCA CGCTCAGCAG ACCCTCCACC CTCCTGGCAA CATCCCTGAG 
ACTGGGCCAA ATCAGCTGTT ACAGCCCCTT AAGCCATCTC CCTCCAGTGA CAACCTCTAC 
TCAGCCTTTA CCAGTGATGG TGCCATTTCA GTCCCAAGCC TTTCTGCTCC AGGTCAAGGG 
ACCAGCAGCA CAAACACTGT CGGGGGAACA GTGAACAGCC AAGCTGCCCC AGCTCAGCCT 
CCTGCCATGA CATCCAGCAG GAAGGGCACA TTCACAGATG ACCTACACAA GTTGGTAGAC 
AATTGGGCCC GAGATGCCAT GAATCTCTCA GGCAGAAGAG GAAGCAAAGG ACACATGAAT 
TATGAGGGCC CTGGAATGGC AAGAAAGTTC TCCGCACCTG GTCAGCTGTG CATCTCCATG 
ACCTCAAATC TGGGTGGTTC TGCCCCCATC TCTGCAGCAT CAGCTACCTC TCTAGGTCAC 
TTCACCAAGT CTATGTGCCC CCCACAGCAG TACGGTTTTC CAGCTCCCCC CTTTGGCACT 
CAGTGGAGTG GGACGGGTGG CCCGGCACCA CAGCCACTTG GCCAGTTCCA GCCTGTGGGA 
ACTGCCTCCT TACAGAATTT CAACATCAGC AATTTGCAGA AATCCATCAG CAACCCCCCA 
GGTTCCAACC TGCGGACCAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963813.1
CDS	99..7262
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVGQSQGQPSSSSLTGIPSSLPIQHSQQQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQLPVSQPVPTIQGEPQIPVAAQPSVVPVHSGAHFLPVGQPLSTSLLPQYPVSQIPISTPHASAPQTGFSSLPITMAAGINQPLLTLASSATAAAIPGGPTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPSRLPPQYPGDSNIAPSSSVASICIPSTVLSPPMPTEALATPGYFPTVVQPFVESNLLVPVGSIGGQVQVSQPAVSLAQQAPTTSSQQAALESTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_014108338.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108338.1

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG 
CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT 
CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC 
CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA 
GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG 
GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT 
CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA 
CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT 
CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA 
GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA 
TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG 
CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG 
TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT 
CTGGAGAGCA CTCAGGGAGT CTCTCAAGTT GCTCCTCCAG AGCCAGTTCC AGTGGCACAG 
GCACAGCCTA CCACTTTGGT CTCCTCTATA GACAGTGCAC ATTCAGATGT TGCTTCAGGA 
ATGAGTGATG GCAATGAGAA TGTCCCGTCC TCCAGTGGAA GGCATGAAGG GAGAGCTACA 
AAACGACATT ACCGAAAATC TGTAAGAAGT CGCTCTCGTC ATGAAAAGAC TTCGCGTCCA 
AAATTAAGAA TTTTGAATGT TTCAAATAAA GGAGACCGAG TAGTAGAATG TCAACTAGAG 
ACTCATAATC GGAAAATGGT TACATTCAAA TTTGATTTAG ATGGTGACAA TCCTGAGGAG 
ATAGCAACAA TTATGGTGAA CAATGACTTT ATTCTAGCAA TGGAGAGAGA GTCCTTTGTG 
GATCAAGTAC GAGAAATTAT TGAAAAGGCT GATGAAATGC TCAGTGAGGA TGTCAGTGTG 
GAACCAGAGG GTGACCAGGG ATTGGAGAGT CTGCAGGGAA AGGATGACTA TGGCTTTACA 
GGTTCTCAAA AATTGGAAGG AGAATTCAAA CAACCAGTTC CTGCGTCTTC CATGCCACAG 
CAAATAGGCA TTCCTACCAG TTCTTTAACT CAAGTTGTTC ATTCTGCTGG AAGACGGTTT 
ATAGTGAGTC CTGTGCCAGA AAGTCGATTA CGAGAATCAA AAGTTTTCAC TAGTGAAATA 
CCAGATACAG TTGCTGCCTC TACTCACAGC GCTGGAATGA ACTTGTCTCA CTCTGCTTCA 
TCCCTTAGCC TACAGCAAGC CTTTTCTGAA CTTAGACATG TGCAGATGAC AGAAGGGCCC 
AGCACAGCAC CTCCCAACTT CAGTCATACA GGACCAACAT TTCCAGTTGT TCCTCCTTCC 
ATGAGTAGCA TTGCTGGAGT CCCAACCACA GCAGTAGCCA CACCTTCAGT ATCAGTCCCT 
GCAACTAGCA GCCCTCTAAA TGACAGTTCC ACATCAGTAA TTCAATCTGA GATTCCAGTG 
CCCACTGAAA CAGGGGTTGG TGGAGTTCCC ACCTGCACAA GTGTAATGCC TTCAAGTGGT 
CTCCCTGTAC CACCCATATC TGAATCGCCA ATATTTTCCA CTATGGTTTC AAGCATCACA 
GTACCTACAG TTGTCTCAAT ATCAGCAACA TCCCAGCCAG TTCAAGCTCC CACATCTGGG 
AGCACTGTTT CTAGCATAGG AACTTTGCCT TCTATACCAG TTTCAACAGC TTTAGCCTCT 
GCAGTGGGCA GTGTTGCTAT CCCAGGTGCT AAGCCTCCAC CTGTACTATC ACAGCAGGCA 
GCAGGCAGTA CTACTGGGGC AGCCACATTA ACATCCGTTC CTGCCATCAC TCCGTTCCCA 
AGCATAGCTT CACAGCCATC TCTTCAGCTT AGCAGCAGCA CCTCTGCTCC AACTCTAGCT 
GAAACAGTGG TGGTTAGTGC ACACTCACTA GATAAAACAT CTCATAGCAG TACAACTGGA 
TTGGCTTTGT CCCTGTCTGC ATCATCTTCC TCCCCTGGAG CAGGATTATC TAGTTCTATT 
TCTCAGCCTG GTGGGGTGCA TCCTTTGGTT ACCCCATCAG CTATGGCTTC TATTCCTGCC 
CTTCCTCAAA CAGCAGGACC TACTTCTACA CCTTTATTGC CCCAAGTACT TAGTATCCCA 
CCCTTGGTAC AACCTGTTGC CAGTGTGCCT GCTGTGCAGC AGACACTAAT TCATAGTCAG 
CCTCAACCAG CTTTACTTCC CAACCAGCCC CACACTCACT GTCCTGAAAT AGATGCTGAC 
TTACAGCCCA AAGCTCCTGG AATTGATGAC ATAAAGACTC TAGAGGAAAA GCTGCGGTCT 
CTCTTTAGTG AACCGAGCTC ATCTGGAGCC CAACATACAT CGGTCTCACT AGAGACTTCA 
CTGGTAGTAG AGACCACTGT CACGCCAGGT ATCCCAACCA CTGCTATTGC ACCAAGCAAG 
CTCATGACAT CTACTACAAG TACATGCTTA CCACCAACCA GTTTGCAATT AGGAACATCT 
GGTTTGTCAG TTATACCAGT GGTCACGCCT GGGCAGGTTT CTACCCCAGT CAGCTATGTG 
TCAGCCCCAG GCAGCACTAC ATCAGGAGTG AAAGCTGGAA CTGCTCCATC AAAGCCACCT 
TTGACTAAAG CTCCAGTGCT GCCAGTGGGT ACTGAACTTC CAGCTGGTAC TCCACCCAGC 
GAGCAGCTGC CACCTTTTCC AGGACCTTCA CTAACTCAGT CCCAGCAACC TCTGGAAGAT 
CTTGATGCTC AGTTGAGAAG AACACTTAGT CCAGAGACTA TTGCGGTGAC ATCTACAATT 
GGTCCTGTGT CCATGGTGGC TCCAACAGCA GTCACAGAAG CAGGAGCACA GCCTCAGAAG 
GATGTTTCTC AGATCACAGA AGGTCCTGTC CTAGCGACTA GTTCGGGAAC TGGTGTTTTT 
AAGATGGGAA GATTTCAGGT TTCAGTTGCA ATGGATGATG TTCAAAAGGA GGGTAAAAAT 
AAGTTAGAAG ATGGAAAGTC TGTTCATTTT GAATCCAGCA CTTCAGAGTC CTCAGTGCTA 
TCAAGTAGTA GTCCAGAAAG TACCCTGGTG AAGACAGAGC CTAATGGGAT AACCATCCCT 
GGCATCTCAT CAGATATGCC AGATAGTGCC CACAAAACTC CTGCCTCAGA AGCAAAGTCA 
GAAACTGGGC AGCCTACCAA GGTTGGACGT TTTCAGGTGA CAACTACAGC AAACAAAGTA 
GGTCGTTTCT CTGTGTCAAG AACTGAAGAC AAGCTCATTG AGGCAAAGAA AGAGGGACCA 
ATAGCGTCTC CTCCTTTTAT GGATTTGGAA CAAGGTGTTC TTCCAGCTGT GATACCAAAG 
AAAGAAAAGC CTGAACTGTC AGAGCCTTCA CATCTGAACG GACCATCTTC TGACCTGGAG 
GCTGCCTTCC TGAGCAGGGA TGTGGATGAT GGTTCAGGTA GTCCACACTC CCCCCATCAG 
CTGAGCTCAA AGAGCCTGCC TATCCAGAAT CTAAGTCAAA GCCTTAGTAA TTCATTCAAC 
TCCTCTTACA TGAGTAGTGA CAATGAGTCA GATATTGAAG ATGAAGATTT AAAATTAGAG 
CTGCGAAGAC TACGAGAAAA ACATCTTAAA GAGATTCAAG ACCTGCAGAG TCGCCAGAAG 
CATGAAATTG AATCTTTGTA TACCAAACTG GGAAAGGTTC CCCCTGCTGT CATTATCCCC 
CCAGCTGCCC CCCTTTCAGG GAGAAGAAGG CGACCCACTA AAAGCAAAGG CAGCAAGTCT 
AGTCGCAGCA GTTCCTTGGG GAATAAAAGT CCCCAGCTTT CAGGTAACCT GTCTGGTCAG 
AGTGCTGCTT CAGTTTTGCA CGCTCAGCAG ACCCTCCACC CTCCTGGCAA CATCCCTGAG 
ACTGGGCCAA ATCAGCTGTT ACAGCCCCTT AAGCCATCTC CCTCCAGTGA CAACCTCTAC 
TCAGCCTTTA CCAGTGATGG TGCCATTTCA GTCCCAAGCC TTTCTGCTCC AGGTCAAGGG 
ACCAGCAGCA CAAACACTGT CGGGGGAACA GTGAACAGCC AAGCTGCCCC AGCTCAGCCT 
CCTGCCATGA CATCCAGCAG GAAGGGCACA TTCACAGATG ACCTACACAA GTTGGTAGAC 
AATTGGGCCC GAGATGCCAT GAATCTCTCA GGCAGAAGAG GAAGCAAAGG ACACATGAAT 
TATGAGGGCC CTGGAATGGC AAGAAAGTTC TCCGCACCTG GTCAGCTGTG CATCTCCATG 
ACCTCAAATC TGGGTGGTTC TGCCCCCATC TCTGCAGCAT CAGCTACCTC TCTAGGTCAC 
TTCACCAAGT CTATGTGCCC CCCACAGCAG TACGGTTTTC CAGCTCCCCC CTTTGGCACT 
CAGTGGAGTG GGACGGGTGG CCCGGCACCA CAGCCACTTG GCCAGTTCCA GCCTGTGGGA 
ACTGCCTCCT TACAGAATTT CAACATCAGC AATTTGCAGA AATCCATCAG CAACCCCCCA 
GGTTCCAACC TGCGGACCAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_014108338.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963813.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X11, mRNA.

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CloneID	OCe43582
Clone ID Related Accession (Same CDS sequence)	XM_014108338.1 , XM_014108338.2
Accession Version	XM_014108338.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase WNK1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014108338.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG 
CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT 
CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC 
CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA 
GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG 
GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT 
CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA 
CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT 
CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA 
GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA 
TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG 
CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG 
TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT 
CTGGAGAGCA CTCAGGGAGT CTCTCAAGTT GCTCCTCCAG AGCCAGTTCC AGTGGCACAG 
GCACAGCCTA CCACTTTGGT CTCCTCTATA GACAGTGCAC ATTCAGATGT TGCTTCAGGA 
ATGAGTGATG GCAATGAGAA TGTCCCGTCC TCCAGTGGAA GGCATGAAGG GAGAGCTACA 
AAACGACATT ACCGAAAATC TGTAAGAAGT CGCTCTCGTC ATGAAAAGAC TTCGCGTCCA 
AAATTAAGAA TTTTGAATGT TTCAAATAAA GGAGACCGAG TAGTAGAATG TCAACTAGAG 
ACTCATAATC GGAAAATGGT TACATTCAAA TTTGATTTAG ATGGTGACAA TCCTGAGGAG 
ATAGCAACAA TTATGGTGAA CAATGACTTT ATTCTAGCAA TGGAGAGAGA GTCCTTTGTG 
GATCAAGTAC GAGAAATTAT TGAAAAGGCT GATGAAATGC TCAGTGAGGA TGTCAGTGTG 
GAACCAGAGG GTGACCAGGG ATTGGAGAGT CTGCAGGGAA AGGATGACTA TGGCTTTACA 
GGTTCTCAAA AATTGGAAGG AGAATTCAAA CAACCAGTTC CTGCGTCTTC CATGCCACAG 
CAAATAGGCA TTCCTACCAG TTCTTTAACT CAAGTTGTTC ATTCTGCTGG AAGACGGTTT 
ATAGTGAGTC CTGTGCCAGA AAGTCGATTA CGAGAATCAA AAGTTTTCAC TAGTGAAATA 
CCAGATACAG TTGCTGCCTC TACTCACAGC GCTGGAATGA ACTTGTCTCA CTCTGCTTCA 
TCCCTTAGCC TACAGCAAGC CTTTTCTGAA CTTAGACATG TGCAGATGAC AGAAGGGCCC 
AGCACAGCAC CTCCCAACTT CAGTCATACA GGACCAACAT TTCCAGTTGT TCCTCCTTCC 
ATGAGTAGCA TTGCTGGAGT CCCAACCACA GCAGTAGCCA CACCTTCAGT ATCAGTCCCT 
GCAACTAGCA GCCCTCTAAA TGACAGTTCC ACATCAGTAA TTCAATCTGA GATTCCAGTG 
CCCACTGAAA CAGGGGTTGG TGGAGTTCCC ACCTGCACAA GTGTAATGCC TTCAAGTGGT 
CTCCCTGTAC CACCCATATC TGAATCGCCA ATATTTTCCA CTATGGTTTC AAGCATCACA 
GTACCTACAG TTGTCTCAAT ATCAGCAACA TCCCAGCCAG TTCAAGCTCC CACATCTGGG 
AGCACTGTTT CTAGCATAGG AACTTTGCCT TCTATACCAG TTTCAACAGC TTTAGCCTCT 
GCAGTGGGCA GTGTTGCTAT CCCAGGTGCT AAGCCTCCAC CTGTACTATC ACAGCAGGCA 
GCAGGCAGTA CTACTGGGGC AGCCACATTA ACATCCGTTC CTGCCATCAC TCCGTTCCCA 
AGCATAGCTT CACAGCCATC TCTTCAGCTT AGCAGCAGCA CCTCTGCTCC AACTCTAGCT 
GAAACAGTGG TGGTTAGTGC ACACTCACTA GATAAAACAT CTCATAGCAG TACAACTGGA 
TTGGCTTTGT CCCTGTCTGC ATCATCTTCC TCCCCTGGAG CAGGATTATC TAGTTCTATT 
TCTCAGCCTG GTGGGGTGCA TCCTTTGGTT ACCCCATCAG CTATGGCTTC TATTCCTGCC 
CTTCCTCAAA CAGCAGGACC TACTTCTACA CCTTTATTGC CCCAAGTACT TAGTATCCCA 
CCCTTGGTAC AACCTGTTGC CAGTGTGCCT GCTGTGCAGC AGACACTAAT TCATAGTCAG 
CCTCAACCAG CTTTACTTCC CAACCAGCCC CACACTCACT GTCCTGAAAT AGATGCTGAC 
TTACAGCCCA AAGCTCCTGG AATTGATGAC ATAAAGACTC TAGAGGAAAA GCTGCGGTCT 
CTCTTTAGTG AACCGAGCTC ATCTGGAGCC CAACATACAT CGGTCTCACT AGAGACTTCA 
CTGGTAGTAG AGACCACTGT CACGCCAGGT ATCCCAACCA CTGCTATTGC ACCAAGCAAG 
CTCATGACAT CTACTACAAG TACATGCTTA CCACCAACCA GTTTGCAATT AGGAACATCT 
GGTTTGTCAG TTATACCAGT GGTCACGCCT GGGCAGGTTT CTACCCCAGT CAGCTATGTG 
TCAGCCCCAG GCAGCACTAC ATCAGGAGTG AAAGCTGGAA CTGCTCCATC AAAGCCACCT 
TTGACTAAAG CTCCAGTGCT GCCAGTGGGT ACTGAACTTC CAGCTGGTAC TCCACCCAGC 
GAGCAGCTGC CACCTTTTCC AGGACCTTCA CTAACTCAGT CCCAGCAACC TCTGGAAGAT 
CTTGATGCTC AGTTGAGAAG AACACTTAGT CCAGAGACTA TTGCGGTGAC ATCTACAATT 
GGTCCTGTGT CCATGGTGGC TCCAACAGCA GTCACAGAAG CAGGAGCACA GCCTCAGAAG 
GATGTTTCTC AGATCACAGA AGGTCCTGTC CTAGCGACTA GTTCGGGAAC TGGTGTTTTT 
AAGATGGGAA GATTTCAGGT TTCAGTTGCA ATGGATGATG TTCAAAAGGA GGGTAAAAAT 
AAGTTAGAAG ATGGAAAGTC TGTTCATTTT GAATCCAGCA CTTCAGAGTC CTCAGTGCTA 
TCAAGTAGTA GTCCAGAAAG TACCCTGGTG AAGACAGAGC CTAATGGGAT AACCATCCCT 
GGCATCTCAT CAGATATGCC AGATAGTGCC CACAAAACTC CTGCCTCAGA AGCAAAGTCA 
GAAACTGGGC AGCCTACCAA GGTTGGACGT TTTCAGGTGA CAACTACAGC AAACAAAGTA 
GGTCGTTTCT CTGTGTCAAG AACTGAAGAC AAGCTCATTG AGGCAAAGAA AGAGGGACCA 
ATAGCGTCTC CTCCTTTTAT GGATTTGGAA CAAGGTGTTC TTCCAGCTGT GATACCAAAG 
AAAGAAAAGC CTGAACTGTC AGAGCCTTCA CATCTGAACG GACCATCTTC TGACCTGGAG 
GCTGCCTTCC TGAGCAGGGA TGTGGATGAT GGTTCAGGTA GTCCACACTC CCCCCATCAG 
CTGAGCTCAA AGAGCCTGCC TATCCAGAAT CTAAGTCAAA GCCTTAGTAA TTCATTCAAC 
TCCTCTTACA TGAGTAGTGA CAATGAGTCA GATATTGAAG ATGAAGATTT AAAATTAGAG 
CTGCGAAGAC TACGAGAAAA ACATCTTAAA GAGATTCAAG ACCTGCAGAG TCGCCAGAAG 
CATGAAATTG AATCTTTGTA TACCAAACTG GGAAAGGTTC CCCCTGCTGT CATTATCCCC 
CCAGCTGCCC CCCTTTCAGG GAGAAGAAGG CGACCCACTA AAAGCAAAGG CAGCAAGTCT 
AGTCGCAGCA GTTCCTTGGG GAATAAAAGT CCCCAGCTTT CAGGTAACCT GTCTGGTCAG 
AGTGCTGCTT CAGTTTTGCA CGCTCAGCAG ACCCTCCACC CTCCTGGCAA CATCCCTGAG 
ACTGGGCCAA ATCAGCTGTT ACAGCCCCTT AAGCCATCTC CCTCCAGTGA CAACCTCTAC 
TCAGCCTTTA CCAGTGATGG TGCCATTTCA GTCCCAAGCC TTTCTGCTCC AGGTCAAGGG 
ACCAGCAGCA CAAACACTGT CGGGGGAACA GTGAACAGCC AAGCTGCCCC AGCTCAGCCT 
CCTGCCATGA CATCCAGCAG GAAGGGCACA TTCACAGATG ACCTACACAA GTTGGTAGAC 
AATTGGGCCC GAGATGCCAT GAATCTCTCA GGCAGAAGAG GAAGCAAAGG ACACATGAAT 
TATGAGGGCC CTGGAATGGC AAGAAAGTTC TCCGCACCTG GTCAGCTGTG CATCTCCATG 
ACCTCAAATC TGGGTGGTTC TGCCCCCATC TCTGCAGCAT CAGCTACCTC TCTAGGTCAC 
TTCACCAAGT CTATGTGCCC CCCACAGCAG TACGGTTTTC CAGCTCCCCC CTTTGGCACT 
CAGTGGAGTG GGACGGGTGG CCCGGCACCA CAGCCACTTG GCCAGTTCCA GCCTGTGGGA 
ACTGCCTCCT TACAGAATTT CAACATCAGC AATTTGCAGA AATCCATCAG CAACCCCCCA 
GGTTCCAACC TGCGGACCAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963813.1
CDS	995..8158
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVGQSQGQPSSSSLTGIPSSLPIQHSQQQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQLPVSQPVPTIQGEPQIPVAAQPSVVPVHSGAHFLPVGQPLSTSLLPQYPVSQIPISTPHASAPQTGFSSLPITMAAGINQPLLTLASSATAAAIPGGPTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLPSGDVLYQGFPSRLPPQYPGDSNIAPSSSVASICIPSTVLSPPMPTEALATPGYFPTVVQPFVESNLLVPVGSIGGQVQVSQPAVSLAQQAPTTSSQQAALESTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_014108338.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108338.2

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGCTTCC AGTTTCCCAG 
CCAGTACCAA CTATCCAAGG CGAACCTCAG ATCCCAGTTG CGGCACAACC CTCAGTTGTT 
CCAGTCCACT CTGGTGCTCA TTTCCTCCCT GTGGGACAGC CACTCTCTAC TTCCTTACTC 
CCTCAGTACC CGGTCTCTCA AATTCCCATA TCAACTCCTC ATGCGTCTGC GCCCCAGACA 
GGTTTTTCAT CCCTTCCCAT TACAATGGCA GCTGGCATTA ATCAGCCTCT GCTCACCTTG 
GCTTCATCTG CTACAGCAGC TGCGATCCCA GGGGGACCAA CTGTGGTTCC TAGTCAGCTT 
CCAACCCTTC TGCAGCCTGT GACTCAGCTG CCAAGTCAGG TTCACCCACA ACTCCTGCAA 
CCAGCAGTTC AGTCCATGGG AATACCAGCT AACCTTGGAC AAGCTGCTGA GGTTCCACTT 
CCCTCTGGAG ATGTTCTGTA CCAGGGCTTT CCATCTCGAC TGCCACCACA GTACCCAGGA 
GATTCAAATA TTGCTCCTTC TTCCAGCGTG GCTTCTATTT GCATCCCTTC TACAGTCCTA 
TCCCCTCCCA TGCCCACAGA AGCACTGGCT ACACCGGGTT ACTTTCCTAC AGTGGTGCAG 
CCTTTTGTGG AATCTAACCT TTTGGTTCCT GTGGGCAGTA TAGGAGGACA GGTTCAAGTG 
TCCCAGCCAG CAGTGAGTTT AGCACAACAA GCCCCCACTA CATCCTCCCA GCAAGCAGCT 
CTGGAGAGCA CTCAGGGAGT CTCTCAAGTT GCTCCTCCAG AGCCAGTTCC AGTGGCACAG 
GCACAGCCTA CCACTTTGGT CTCCTCTATA GACAGTGCAC ATTCAGATGT TGCTTCAGGA 
ATGAGTGATG GCAATGAGAA TGTCCCGTCC TCCAGTGGAA GGCATGAAGG GAGAGCTACA 
AAACGACATT ACCGAAAATC TGTAAGAAGT CGCTCTCGTC ATGAAAAGAC TTCGCGTCCA 
AAATTAAGAA TTTTGAATGT TTCAAATAAA GGAGACCGAG TAGTAGAATG TCAACTAGAG 
ACTCATAATC GGAAAATGGT TACATTCAAA TTTGATTTAG ATGGTGACAA TCCTGAGGAG 
ATAGCAACAA TTATGGTGAA CAATGACTTT ATTCTAGCAA TGGAGAGAGA GTCCTTTGTG 
GATCAAGTAC GAGAAATTAT TGAAAAGGCT GATGAAATGC TCAGTGAGGA TGTCAGTGTG 
GAACCAGAGG GTGACCAGGG ATTGGAGAGT CTGCAGGGAA AGGATGACTA TGGCTTTACA 
GGTTCTCAAA AATTGGAAGG AGAATTCAAA CAACCAGTTC CTGCGTCTTC CATGCCACAG 
CAAATAGGCA TTCCTACCAG TTCTTTAACT CAAGTTGTTC ATTCTGCTGG AAGACGGTTT 
ATAGTGAGTC CTGTGCCAGA AAGTCGATTA CGAGAATCAA AAGTTTTCAC TAGTGAAATA 
CCAGATACAG TTGCTGCCTC TACTCACAGC GCTGGAATGA ACTTGTCTCA CTCTGCTTCA 
TCCCTTAGCC TACAGCAAGC CTTTTCTGAA CTTAGACATG TGCAGATGAC AGAAGGGCCC 
AGCACAGCAC CTCCCAACTT CAGTCATACA GGACCAACAT TTCCAGTTGT TCCTCCTTCC 
ATGAGTAGCA TTGCTGGAGT CCCAACCACA GCAGTAGCCA CACCTTCAGT ATCAGTCCCT 
GCAACTAGCA GCCCTCTAAA TGACAGTTCC ACATCAGTAA TTCAATCTGA GATTCCAGTG 
CCCACTGAAA CAGGGGTTGG TGGAGTTCCC ACCTGCACAA GTGTAATGCC TTCAAGTGGT 
CTCCCTGTAC CACCCATATC TGAATCGCCA ATATTTTCCA CTATGGTTTC AAGCATCACA 
GTACCTACAG TTGTCTCAAT ATCAGCAACA TCCCAGCCAG TTCAAGCTCC CACATCTGGG 
AGCACTGTTT CTAGCATAGG AACTTTGCCT TCTATACCAG TTTCAACAGC TTTAGCCTCT 
GCAGTGGGCA GTGTTGCTAT CCCAGGTGCT AAGCCTCCAC CTGTACTATC ACAGCAGGCA 
GCAGGCAGTA CTACTGGGGC AGCCACATTA ACATCCGTTC CTGCCATCAC TCCGTTCCCA 
AGCATAGCTT CACAGCCATC TCTTCAGCTT AGCAGCAGCA CCTCTGCTCC AACTCTAGCT 
GAAACAGTGG TGGTTAGTGC ACACTCACTA GATAAAACAT CTCATAGCAG TACAACTGGA 
TTGGCTTTGT CCCTGTCTGC ATCATCTTCC TCCCCTGGAG CAGGATTATC TAGTTCTATT 
TCTCAGCCTG GTGGGGTGCA TCCTTTGGTT ACCCCATCAG CTATGGCTTC TATTCCTGCC 
CTTCCTCAAA CAGCAGGACC TACTTCTACA CCTTTATTGC CCCAAGTACT TAGTATCCCA 
CCCTTGGTAC AACCTGTTGC CAGTGTGCCT GCTGTGCAGC AGACACTAAT TCATAGTCAG 
CCTCAACCAG CTTTACTTCC CAACCAGCCC CACACTCACT GTCCTGAAAT AGATGCTGAC 
TTACAGCCCA AAGCTCCTGG AATTGATGAC ATAAAGACTC TAGAGGAAAA GCTGCGGTCT 
CTCTTTAGTG AACCGAGCTC ATCTGGAGCC CAACATACAT CGGTCTCACT AGAGACTTCA 
CTGGTAGTAG AGACCACTGT CACGCCAGGT ATCCCAACCA CTGCTATTGC ACCAAGCAAG 
CTCATGACAT CTACTACAAG TACATGCTTA CCACCAACCA GTTTGCAATT AGGAACATCT 
GGTTTGTCAG TTATACCAGT GGTCACGCCT GGGCAGGTTT CTACCCCAGT CAGCTATGTG 
TCAGCCCCAG GCAGCACTAC ATCAGGAGTG AAAGCTGGAA CTGCTCCATC AAAGCCACCT 
TTGACTAAAG CTCCAGTGCT GCCAGTGGGT ACTGAACTTC CAGCTGGTAC TCCACCCAGC 
GAGCAGCTGC CACCTTTTCC AGGACCTTCA CTAACTCAGT CCCAGCAACC TCTGGAAGAT 
CTTGATGCTC AGTTGAGAAG AACACTTAGT CCAGAGACTA TTGCGGTGAC ATCTACAATT 
GGTCCTGTGT CCATGGTGGC TCCAACAGCA GTCACAGAAG CAGGAGCACA GCCTCAGAAG 
GATGTTTCTC AGATCACAGA AGGTCCTGTC CTAGCGACTA GTTCGGGAAC TGGTGTTTTT 
AAGATGGGAA GATTTCAGGT TTCAGTTGCA ATGGATGATG TTCAAAAGGA GGGTAAAAAT 
AAGTTAGAAG ATGGAAAGTC TGTTCATTTT GAATCCAGCA CTTCAGAGTC CTCAGTGCTA 
TCAAGTAGTA GTCCAGAAAG TACCCTGGTG AAGACAGAGC CTAATGGGAT AACCATCCCT 
GGCATCTCAT CAGATATGCC AGATAGTGCC CACAAAACTC CTGCCTCAGA AGCAAAGTCA 
GAAACTGGGC AGCCTACCAA GGTTGGACGT TTTCAGGTGA CAACTACAGC AAACAAAGTA 
GGTCGTTTCT CTGTGTCAAG AACTGAAGAC AAGCTCATTG AGGCAAAGAA AGAGGGACCA 
ATAGCGTCTC CTCCTTTTAT GGATTTGGAA CAAGGTGTTC TTCCAGCTGT GATACCAAAG 
AAAGAAAAGC CTGAACTGTC AGAGCCTTCA CATCTGAACG GACCATCTTC TGACCTGGAG 
GCTGCCTTCC TGAGCAGGGA TGTGGATGAT GGTTCAGGTA GTCCACACTC CCCCCATCAG 
CTGAGCTCAA AGAGCCTGCC TATCCAGAAT CTAAGTCAAA GCCTTAGTAA TTCATTCAAC 
TCCTCTTACA TGAGTAGTGA CAATGAGTCA GATATTGAAG ATGAAGATTT AAAATTAGAG 
CTGCGAAGAC TACGAGAAAA ACATCTTAAA GAGATTCAAG ACCTGCAGAG TCGCCAGAAG 
CATGAAATTG AATCTTTGTA TACCAAACTG GGAAAGGTTC CCCCTGCTGT CATTATCCCC 
CCAGCTGCCC CCCTTTCAGG GAGAAGAAGG CGACCCACTA AAAGCAAAGG CAGCAAGTCT 
AGTCGCAGCA GTTCCTTGGG GAATAAAAGT CCCCAGCTTT CAGGTAACCT GTCTGGTCAG 
AGTGCTGCTT CAGTTTTGCA CGCTCAGCAG ACCCTCCACC CTCCTGGCAA CATCCCTGAG 
ACTGGGCCAA ATCAGCTGTT ACAGCCCCTT AAGCCATCTC CCTCCAGTGA CAACCTCTAC 
TCAGCCTTTA CCAGTGATGG TGCCATTTCA GTCCCAAGCC TTTCTGCTCC AGGTCAAGGG 
ACCAGCAGCA CAAACACTGT CGGGGGAACA GTGAACAGCC AAGCTGCCCC AGCTCAGCCT 
CCTGCCATGA CATCCAGCAG GAAGGGCACA TTCACAGATG ACCTACACAA GTTGGTAGAC 
AATTGGGCCC GAGATGCCAT GAATCTCTCA GGCAGAAGAG GAAGCAAAGG ACACATGAAT 
TATGAGGGCC CTGGAATGGC AAGAAAGTTC TCCGCACCTG GTCAGCTGTG CATCTCCATG 
ACCTCAAATC TGGGTGGTTC TGCCCCCATC TCTGCAGCAT CAGCTACCTC TCTAGGTCAC 
TTCACCAAGT CTATGTGCCC CCCACAGCAG TACGGTTTTC CAGCTCCCCC CTTTGGCACT 
CAGTGGAGTG GGACGGGTGG CCCGGCACCA CAGCCACTTG GCCAGTTCCA GCCTGTGGGA 
ACTGCCTCCT TACAGAATTT CAACATCAGC AATTTGCAGA AATCCATCAG CAACCCCCCA 
GGTTCCAACC TGCGGACCAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_014108339.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963814.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X15, mRNA.

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CloneID	OCe43583
Clone ID Related Accession (Same CDS sequence)	XM_014108339.1
Accession Version	XM_014108339.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase WNK1 isoform X15
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGGGCTT TCCATCTCGA 
CTGCCACCAC AGTACCCAGG AGATTCAAAT ATTGCTCCTT CTTCCAGCGT GGCTTCTATT 
TGCATCCCTT CTACAGTCCT ATCCCCTCCC ATGCCCACAG AAGCACTGGC TACACCGGGT 
TACTTTCCTA CAGTGGTGCA GCCTTTTGTG GAATCTAACC TTTTGGTTCC TGTGGGCAGT 
ATAGGAGGAC AGGTTCAAGT GTCCCAGCCA GCAGTGAGTT TAGCACAACA AGCCCCCACT 
ACATCCTCCC AGCAAGCAGC TCTGGAGCAG AATGAAATGC CAATGTGGGG GCCTTGTTGC 
CACTGGCTGT TCTGTTGGGA AAGCACTCAG GGAGTCTCTC AAGTTGCTCC TCCAGAGCCA 
GTTCCAGTGG CACAGGCACA GCCTACCACT TTGGTCTCCT CTATAGACAG TGCACATTCA 
GATGTTGCTT CAGGAATGAG TGATGGCAAT GAGAATGTCC CGTCCTCCAG TGGAAGGCAT 
GAAGGGAGAG CTACAAAACG ACATTACCGA AAATCTGTAA GAAGTCGCTC TCGTCATGAA 
AAGACTTCGC GTCCAAAATT AAGAATTTTG AATGTTTCAA ATAAAGGAGA CCGAGTAGTA 
GAATGTCAAC TAGAGACTCA TAATCGGAAA ATGGTTACAT TCAAATTTGA TTTAGATGGT 
GACAATCCTG AGGAGATAGC AACAATTATG GTGAACAATG ACTTTATTCT AGCAATGGAG 
AGAGAGTCCT TTGTGGATCA AGTACGAGAA ATTATTGAAA AGGCTGATGA AATGCTCAGT 
GAGGATGTCA GTGTGGAACC AGAGGGTGAC CAGGGATTGG AGAGTCTGCA GGGAAAGGAT 
GACTATGGCT TTACAGGTTC TCAAAAATTG GAAGGAGAAT TCAAACAACC AGTTCCTGCG 
TCTTCCATGC CACAGCAAAT AGGCATTCCT ACCAGTTCTT TAACTCAAGT TGTTCATTCT 
GCTGGAAGAC GGTTTATAGT GAGTCCTGTG CCAGAAAGTC GATTACGAGA ATCAAAAGTT 
TTCACTAGTG AAATACCAGA TACAGTTGCT GCCTCTACTC ACAGCGCTGG AATGAACTTG 
TCTCACTCTG CTTCATCCCT TAGCCTACAG CAAGCCTTTT CTGAACTTAG ACATGTGCAG 
ATGACAGAAG GGCCCAGCAC AGCACCTCCC AACTTCAGTC ATACAGGACC AACATTTCCA 
GTTGTTCCTC CTTCCATGAG TAGCATTGCT GGAGTCCCAA CCACAGCAGT AGCCACACCT 
TCAGTATCAG TCCCTGCAAC TAGCAGCCCT CTAAATGACA GTTCCACATC AGTAATTCAA 
TCTGAGATTC CAGTGCCCAC TGAAACAGGG GTTGGTGGAG TTCCCACCTG CACAAGTGTA 
ATGCCTTCAA GTGGTCTCCC TGTACCACCC ATATCTGAAT CGCCAATATT TTCCACTATG 
GTTTCAAGCA TCACAGTACC TACAGTTGTC TCAATATCAG CAACATCCCA GCCAGTTCAA 
GCTCCCACAT CTGGGAGCAC TGTTTCTAGC ATAGGAACTT TGCCTTCTAT ACCAGTTTCA 
ACAGCTTTAG CCTCTGCAGT GGGCAGTGTT GCTATCCCAG GTGCTAAGCC TCCACCTGTA 
CTATCACAGC AGGCAGCAGG CAGTACTACT GGGGCAGCCA CATTAACATC CGTTCCTGCC 
ATCACTCCGT TCCCAAGCAT AGCTTCACAG CCATCTCTTC AGCTTAGCAG CAGCACCTCT 
GCTCCAACTC TAGCTGAAAC AGTGGTGGTT AGTGCACACT CACTAGATAA AACATCTCAT 
AGCAGTACAA CTGGATTGGC TTTGTCCCTG TCTGCATCAT CTTCCTCCCC TGGAGCAGGA 
TTATCTAGTT CTATTTCTCA GCCTGGTGGG GTGCATCCTT TGGTTACCCC ATCAGCTATG 
GCTTCTATTC CTGCCCTTCC TCAAACAGCA GGACCTACTT CTACACCTTT ATTGCCCCAA 
GTACTTAGTA TCCCACCCTT GGTACAACCT GTTGCCAGTG TGCCTGCTGT GCAGCAGACA 
CTAATTCATA GTCAGCCTCA ACCAGCTTTA CTTCCCAACC AGCCCCACAC TCACTGTCCT 
GAAATAGATG CTGACTTACA GCCCAAAGCT CCTGGAATTG ATGACATAAA GACTCTAGAG 
GAAAAGCTGC GGTCTCTCTT TAGTGAACCG AGCTCATCTG GAGCCCAACA TACATCGGTC 
TCACTAGAGA CTTCACTGGT AGTAGAGACC ACTGTCACGC CAGGTATCCC AACCACTGCT 
ATTGCACCAA GCAAGCTCAT GACATCTACT ACAAGTACAT GCTTACCACC AACCAGTTTG 
CAATTAGGAA CATCTGGTTT GTCAGTTATA CCAGTGGTCA CGCCTGGGCA GGTTTCTACC 
CCAGTCAGCT ATGTGTCAGC CCCAGGCAGC ACTACATCAG GAGTGAAAGC TGGAACTGCT 
CCATCAAAGC CACCTTTGAC TAAAGCTCCA GTGCTGCCAG TGGGTACTGA ACTTCCAGCT 
GGTACTCCAC CCAGCGAGCA GCTGCCACCT TTTCCAGGAC CTTCACTAAC TCAGTCCCAG 
CAACCTCTGG AAGATCTTGA TGCTCAGTTG AGAAGAACAC TTAGTCCAGA GACTATTGCG 
GTGACATCTA CAATTGGTCC TGTGTCCATG GTGGCTCCAA CAGCAGTCAC AGAAGCAGGA 
GCACAGCCTC AGAAGGATGT TTCTCAGATC ACAGAAGGTC CTGTCCTAGC GACTAGTTCG 
GGAACTGGTG TTTTTAAGAT GGGAAGATTT CAGGTTTCAG TTGCAATGGA TGATGTTCAA 
AAGGAGGGTA AAAATAAGTT AGAAGATGGA AAGTCTGTTC ATTTTGAATC CAGCACTTCA 
GAGTCCTCAG TGCTATCAAG TAGTAGTCCA GAAAGTACCC TGGTGAAGAC AGAGCCTAAT 
GGGATAACCA TCCCTGGCAT CTCATCAGAT ATGCCAGATA GTGCCCACAA AACTCCTGCC 
TCAGAAGCAA AGTCAGAAAC TGGGCAGCCT ACCAAGGTTG GACGTTTTCA GGTGACAACT 
ACAGCAAACA AAGTAGGTCG TTTCTCTGTG TCAAGAACTG AAGACAAGCT CATTGAGGCA 
AAGAAAGAGG GACCAATAGC GTCTCCTCCT TTTATGGATT TGGAACAAGG TGTTCTTCCA 
GCTGTGATAC CAAAGAAAGA AAAGCCTGAA CTGTCAGAGC CTTCACATCT GAACGGACCA 
TCTTCTGACC TGGAGGCTGC CTTCCTGAGC AGGGATGTGG ATGATGGTTC AGGTAGTCCA 
CACTCCCCCC ATCAGCTGAG CTCAAAGAGC CTGCCTATCC AGAATCTAAG TCAAAGCCTT 
AGTAATTCAT TCAACTCCTC TTACATGAGT AGTGACAATG AGTCAGATAT TGAAGATGAA 
GATTTAAAAT TAGAGCTGCG AAGACTACGA GAAAAACATC TTAAAGAGAT TCAAGACCTG 
CAGAGTCGCC AGAAGCATGA AATTGAATCT TTGTATACCA AACTGGGAAA GGTTCCCCCT 
GCTGTCATTA TCCCCCCAGC TGCCCCCCTT TCAGGGAGAA GAAGGCGACC CACTAAAAGC 
AAAGGCAGCA AGTCTAGTCG CAGCAGTTCC TTGGGGAATA AAAGTCCCCA GCTTTCAGGT 
AACCTGTCTG GTCAGAGTGC TGCTTCAGTT TTGCACGCTC AGCAGACCCT CCACCCTCCT 
GGCAACATCC CTGAGACTGG GCCAAATCAG CTGTTACAGC CCCTTAAGCC ATCTCCCTCC 
AGTGACAACC TCTACTCAGC CTTTACCAGT GATGGTGCCA TTTCAGTCCC AAGCCTTTCT 
GCTCCAGGTC AAGGCTGTGC GAAGTTTAAC TGTGCATCTG AGCAGGTGAC ATTCAAACCT 
GGTGGCAGGA GGACCCGATT TCTGAGTACG CCCTGCTTGG CTCTTTGGAA GATGGTGAAA 
AAAGTCTGTC CTTGCAACCA GCTCTGTATG TGCCCTCCAG CAGAGCCGAA ATCAGGGACC 
AGCAGCACAA ACACTGTCGG GGGAACAGTG AACAGCCAAG CTGCCCCAGC TCAGCCTCCT 
GCCATGACAT CCAGCAGGAA GGGCACATTC ACAGATGACC TACACAAGTT GGTAGACAAT 
TGGGCCCGAG ATGCCATGAA TCTCTCAGGC AGAAGAGGAA GCAAAGGACA CATGAATTAT 
GAGGGCCCTG GAATGGCAAG AAAGTTCTCC GCACCTGGTC AGCTGTGCAT CTCCATGACC 
TCAAATCTGG GTGGTTCTGC CCCCATCTCT GCAGCATCAG CTACCTCTCT AGGTCACTTC 
ACCAAGTCTA TGTGCCCCCC ACAGCAGTAC GGTTTTCCAG CTCCCCCCTT TGGCACTCAG 
TGGAGTGGGA CGGGTGGCCC GGCACCACAG CCACTTGGCC AGTTCCAGCC TGTGGGAACT 
GCCTCCTTAC AGAATTTCAA CATCAGCAAT TTGCAGAAAT CCATCAGCAA CCCCCCAGGT 
TCCAACCTGC GGACCACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963814.1
CDS	99..7019
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVGQSQGQPSSSSLTGIPSSLPIQHSQQQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQGFPSRLPPQYPGDSNIAPSSSVASICIPSTVLSPPMPTEALATPGYFPTVVQPFVESNLLVPVGSIGGQVQVSQPAVSLAQQAPTTSSQQAALEQNEMPMWGPCCHWLFCWESTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGCAKFNCASEQVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCMCPPAEPKSGTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_014108339.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108339.1

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGGGCTT TCCATCTCGA 
CTGCCACCAC AGTACCCAGG AGATTCAAAT ATTGCTCCTT CTTCCAGCGT GGCTTCTATT 
TGCATCCCTT CTACAGTCCT ATCCCCTCCC ATGCCCACAG AAGCACTGGC TACACCGGGT 
TACTTTCCTA CAGTGGTGCA GCCTTTTGTG GAATCTAACC TTTTGGTTCC TGTGGGCAGT 
ATAGGAGGAC AGGTTCAAGT GTCCCAGCCA GCAGTGAGTT TAGCACAACA AGCCCCCACT 
ACATCCTCCC AGCAAGCAGC TCTGGAGCAG AATGAAATGC CAATGTGGGG GCCTTGTTGC 
CACTGGCTGT TCTGTTGGGA AAGCACTCAG GGAGTCTCTC AAGTTGCTCC TCCAGAGCCA 
GTTCCAGTGG CACAGGCACA GCCTACCACT TTGGTCTCCT CTATAGACAG TGCACATTCA 
GATGTTGCTT CAGGAATGAG TGATGGCAAT GAGAATGTCC CGTCCTCCAG TGGAAGGCAT 
GAAGGGAGAG CTACAAAACG ACATTACCGA AAATCTGTAA GAAGTCGCTC TCGTCATGAA 
AAGACTTCGC GTCCAAAATT AAGAATTTTG AATGTTTCAA ATAAAGGAGA CCGAGTAGTA 
GAATGTCAAC TAGAGACTCA TAATCGGAAA ATGGTTACAT TCAAATTTGA TTTAGATGGT 
GACAATCCTG AGGAGATAGC AACAATTATG GTGAACAATG ACTTTATTCT AGCAATGGAG 
AGAGAGTCCT TTGTGGATCA AGTACGAGAA ATTATTGAAA AGGCTGATGA AATGCTCAGT 
GAGGATGTCA GTGTGGAACC AGAGGGTGAC CAGGGATTGG AGAGTCTGCA GGGAAAGGAT 
GACTATGGCT TTACAGGTTC TCAAAAATTG GAAGGAGAAT TCAAACAACC AGTTCCTGCG 
TCTTCCATGC CACAGCAAAT AGGCATTCCT ACCAGTTCTT TAACTCAAGT TGTTCATTCT 
GCTGGAAGAC GGTTTATAGT GAGTCCTGTG CCAGAAAGTC GATTACGAGA ATCAAAAGTT 
TTCACTAGTG AAATACCAGA TACAGTTGCT GCCTCTACTC ACAGCGCTGG AATGAACTTG 
TCTCACTCTG CTTCATCCCT TAGCCTACAG CAAGCCTTTT CTGAACTTAG ACATGTGCAG 
ATGACAGAAG GGCCCAGCAC AGCACCTCCC AACTTCAGTC ATACAGGACC AACATTTCCA 
GTTGTTCCTC CTTCCATGAG TAGCATTGCT GGAGTCCCAA CCACAGCAGT AGCCACACCT 
TCAGTATCAG TCCCTGCAAC TAGCAGCCCT CTAAATGACA GTTCCACATC AGTAATTCAA 
TCTGAGATTC CAGTGCCCAC TGAAACAGGG GTTGGTGGAG TTCCCACCTG CACAAGTGTA 
ATGCCTTCAA GTGGTCTCCC TGTACCACCC ATATCTGAAT CGCCAATATT TTCCACTATG 
GTTTCAAGCA TCACAGTACC TACAGTTGTC TCAATATCAG CAACATCCCA GCCAGTTCAA 
GCTCCCACAT CTGGGAGCAC TGTTTCTAGC ATAGGAACTT TGCCTTCTAT ACCAGTTTCA 
ACAGCTTTAG CCTCTGCAGT GGGCAGTGTT GCTATCCCAG GTGCTAAGCC TCCACCTGTA 
CTATCACAGC AGGCAGCAGG CAGTACTACT GGGGCAGCCA CATTAACATC CGTTCCTGCC 
ATCACTCCGT TCCCAAGCAT AGCTTCACAG CCATCTCTTC AGCTTAGCAG CAGCACCTCT 
GCTCCAACTC TAGCTGAAAC AGTGGTGGTT AGTGCACACT CACTAGATAA AACATCTCAT 
AGCAGTACAA CTGGATTGGC TTTGTCCCTG TCTGCATCAT CTTCCTCCCC TGGAGCAGGA 
TTATCTAGTT CTATTTCTCA GCCTGGTGGG GTGCATCCTT TGGTTACCCC ATCAGCTATG 
GCTTCTATTC CTGCCCTTCC TCAAACAGCA GGACCTACTT CTACACCTTT ATTGCCCCAA 
GTACTTAGTA TCCCACCCTT GGTACAACCT GTTGCCAGTG TGCCTGCTGT GCAGCAGACA 
CTAATTCATA GTCAGCCTCA ACCAGCTTTA CTTCCCAACC AGCCCCACAC TCACTGTCCT 
GAAATAGATG CTGACTTACA GCCCAAAGCT CCTGGAATTG ATGACATAAA GACTCTAGAG 
GAAAAGCTGC GGTCTCTCTT TAGTGAACCG AGCTCATCTG GAGCCCAACA TACATCGGTC 
TCACTAGAGA CTTCACTGGT AGTAGAGACC ACTGTCACGC CAGGTATCCC AACCACTGCT 
ATTGCACCAA GCAAGCTCAT GACATCTACT ACAAGTACAT GCTTACCACC AACCAGTTTG 
CAATTAGGAA CATCTGGTTT GTCAGTTATA CCAGTGGTCA CGCCTGGGCA GGTTTCTACC 
CCAGTCAGCT ATGTGTCAGC CCCAGGCAGC ACTACATCAG GAGTGAAAGC TGGAACTGCT 
CCATCAAAGC CACCTTTGAC TAAAGCTCCA GTGCTGCCAG TGGGTACTGA ACTTCCAGCT 
GGTACTCCAC CCAGCGAGCA GCTGCCACCT TTTCCAGGAC CTTCACTAAC TCAGTCCCAG 
CAACCTCTGG AAGATCTTGA TGCTCAGTTG AGAAGAACAC TTAGTCCAGA GACTATTGCG 
GTGACATCTA CAATTGGTCC TGTGTCCATG GTGGCTCCAA CAGCAGTCAC AGAAGCAGGA 
GCACAGCCTC AGAAGGATGT TTCTCAGATC ACAGAAGGTC CTGTCCTAGC GACTAGTTCG 
GGAACTGGTG TTTTTAAGAT GGGAAGATTT CAGGTTTCAG TTGCAATGGA TGATGTTCAA 
AAGGAGGGTA AAAATAAGTT AGAAGATGGA AAGTCTGTTC ATTTTGAATC CAGCACTTCA 
GAGTCCTCAG TGCTATCAAG TAGTAGTCCA GAAAGTACCC TGGTGAAGAC AGAGCCTAAT 
GGGATAACCA TCCCTGGCAT CTCATCAGAT ATGCCAGATA GTGCCCACAA AACTCCTGCC 
TCAGAAGCAA AGTCAGAAAC TGGGCAGCCT ACCAAGGTTG GACGTTTTCA GGTGACAACT 
ACAGCAAACA AAGTAGGTCG TTTCTCTGTG TCAAGAACTG AAGACAAGCT CATTGAGGCA 
AAGAAAGAGG GACCAATAGC GTCTCCTCCT TTTATGGATT TGGAACAAGG TGTTCTTCCA 
GCTGTGATAC CAAAGAAAGA AAAGCCTGAA CTGTCAGAGC CTTCACATCT GAACGGACCA 
TCTTCTGACC TGGAGGCTGC CTTCCTGAGC AGGGATGTGG ATGATGGTTC AGGTAGTCCA 
CACTCCCCCC ATCAGCTGAG CTCAAAGAGC CTGCCTATCC AGAATCTAAG TCAAAGCCTT 
AGTAATTCAT TCAACTCCTC TTACATGAGT AGTGACAATG AGTCAGATAT TGAAGATGAA 
GATTTAAAAT TAGAGCTGCG AAGACTACGA GAAAAACATC TTAAAGAGAT TCAAGACCTG 
CAGAGTCGCC AGAAGCATGA AATTGAATCT TTGTATACCA AACTGGGAAA GGTTCCCCCT 
GCTGTCATTA TCCCCCCAGC TGCCCCCCTT TCAGGGAGAA GAAGGCGACC CACTAAAAGC 
AAAGGCAGCA AGTCTAGTCG CAGCAGTTCC TTGGGGAATA AAAGTCCCCA GCTTTCAGGT 
AACCTGTCTG GTCAGAGTGC TGCTTCAGTT TTGCACGCTC AGCAGACCCT CCACCCTCCT 
GGCAACATCC CTGAGACTGG GCCAAATCAG CTGTTACAGC CCCTTAAGCC ATCTCCCTCC 
AGTGACAACC TCTACTCAGC CTTTACCAGT GATGGTGCCA TTTCAGTCCC AAGCCTTTCT 
GCTCCAGGTC AAGGCTGTGC GAAGTTTAAC TGTGCATCTG AGCAGGTGAC ATTCAAACCT 
GGTGGCAGGA GGACCCGATT TCTGAGTACG CCCTGCTTGG CTCTTTGGAA GATGGTGAAA 
AAAGTCTGTC CTTGCAACCA GCTCTGTATG TGCCCTCCAG CAGAGCCGAA ATCAGGGACC 
AGCAGCACAA ACACTGTCGG GGGAACAGTG AACAGCCAAG CTGCCCCAGC TCAGCCTCCT 
GCCATGACAT CCAGCAGGAA GGGCACATTC ACAGATGACC TACACAAGTT GGTAGACAAT 
TGGGCCCGAG ATGCCATGAA TCTCTCAGGC AGAAGAGGAA GCAAAGGACA CATGAATTAT 
GAGGGCCCTG GAATGGCAAG AAAGTTCTCC GCACCTGGTC AGCTGTGCAT CTCCATGACC 
TCAAATCTGG GTGGTTCTGC CCCCATCTCT GCAGCATCAG CTACCTCTCT AGGTCACTTC 
ACCAAGTCTA TGTGCCCCCC ACAGCAGTAC GGTTTTCCAG CTCCCCCCTT TGGCACTCAG 
TGGAGTGGGA CGGGTGGCCC GGCACCACAG CCACTTGGCC AGTTCCAGCC TGTGGGAACT 
GCCTCCTTAC AGAATTTCAA CATCAGCAAT TTGCAGAAAT CCATCAGCAA CCCCCCAGGT 
TCCAACCTGC GGACCACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_014108340.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963815.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X16, mRNA.

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CloneID	OCe43584
Clone ID Related Accession (Same CDS sequence)	XM_014108340.1
Accession Version	XM_014108340.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase WNK1 isoform X16
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGGG AGTACAGCAG ACAGCCCCTC CACAACAGAC AGCGCAGTAT 
TCGCTTCCAC AGACATCAGC CTCCAGTGAG GCCACTATTG CACAACCAGT CAGTCAGTCT 
CAGGCTCCAC AGGTCTTGCC TCAAATATCA GCTGGAAAAC AGGGCTTTCC ATCTCGACTG 
CCACCACAGT ACCCAGGAGA TTCAAATATT GCTCCTTCTT CCAGCGTGGC TTCTATTTGC 
ATCCCTTCTA CAGTCCTATC CCCTCCCATG CCCACAGAAG CACTGGCTAC ACCGGGTTAC 
TTTCCTACAG TGGTGCAGCC TTTTGTGGAA TCTAACCTTT TGGTTCCTGT GGGCAGTATA 
GGAGGACAGG TTCAAGTGTC CCAGCCAGCA GTGAGTTTAG CACAACAAGC CCCCACTACA 
TCCTCCCAGC AAGCAGCTCT GGAGAGCACT CAGGGAGTCT CTCAAGTTGC TCCTCCAGAG 
CCAGTTCCAG TGGCACAGGC ACAGCCTACC ACTTTGGTCT CCTCTATAGA CAGTGCACAT 
TCAGATGTTG CTTCAGGAAT GAGTGATGGC AATGAGAATG TCCCGTCCTC CAGTGGAAGG 
CATGAAGGGA GAGCTACAAA ACGACATTAC CGAAAATCTG TAAGAAGTCG CTCTCGTCAT 
GAAAAGACTT CGCGTCCAAA ATTAAGAATT TTGAATGTTT CAAATAAAGG AGACCGAGTA 
GTAGAATGTC AACTAGAGAC TCATAATCGG AAAATGGTTA CATTCAAATT TGATTTAGAT 
GGTGACAATC CTGAGGAGAT AGCAACAATT ATGGTGAACA ATGACTTTAT TCTAGCAATG 
GAGAGAGAGT CCTTTGTGGA TCAAGTACGA GAAATTATTG AAAAGGCTGA TGAAATGCTC 
AGTGAGGATG TCAGTGTGGA ACCAGAGGGT GACCAGGGAT TGGAGAGTCT GCAGGGAAAG 
GATGACTATG GCTTTACAGG TTCTCAAAAA TTGGAAGGAG AATTCAAACA ACCAGTTCCT 
GCGTCTTCCA TGCCACAGCA AATAGGCATT CCTACCAGTT CTTTAACTCA AGTTGTTCAT 
TCTGCTGGAA GACGGTTTAT AGTGAGTCCT GTGCCAGAAA GTCGATTACG AGAATCAAAA 
GTTTTCACTA GTGAAATACC AGATACAGTT GCTGCCTCTA CTCACAGCGC TGGAATGAAC 
TTGTCTCACT CTGCTTCATC CCTTAGCCTA CAGCAAGCCT TTTCTGAACT TAGACATGTG 
CAGATGACAG AAGGGCCCAG CACAGCACCT CCCAACTTCA GTCATACAGG ACCAACATTT 
CCAGTTGTTC CTCCTTCCAT GAGTAGCATT GCTGGAGTCC CAACCACAGC AGTAGCCACA 
CCTTCAGTAT CAGTCCCTGC AACTAGCAGC CCTCTAAATG ACAGTTCCAC ATCAGTAATT 
CAATCTGAGA TTCCAGTGCC CACTGAAACA GGGGTTGGTG GAGTTCCCAC CTGCACAAGT 
GTAATGCCTT CAAGTGGTCT CCCTGTACCA CCCATATCTG AATCGCCAAT ATTTTCCACT 
ATGGTTTCAA GCATCACAGT ACCTACAGTT GTCTCAATAT CAGCAACATC CCAGCCAGTT 
CAAGCTCCCA CATCTGGGAG CACTGTTTCT AGCATAGGAA CTTTGCCTTC TATACCAGTT 
TCAACAGCTT TAGCCTCTGC AGTGGGCAGT GTTGCTATCC CAGGTGCTAA GCCTCCACCT 
GTACTATCAC AGCAGGCAGC AGGCAGTACT ACTGGGGCAG CCACATTAAC ATCCGTTCCT 
GCCATCACTC CGTTCCCAAG CATAGCTTCA CAGCCATCTC TTCAGCTTAG CAGCAGCACC 
TCTGCTCCAA CTCTAGCTGA AACAGTGGTG GTTAGTGCAC ACTCACTAGA TAAAACATCT 
CATAGCAGTA CAACTGGATT GGCTTTGTCC CTGTCTGCAT CATCTTCCTC CCCTGGAGCA 
GGATTATCTA GTTCTATTTC TCAGCCTGGT GGGGTGCATC CTTTGGTTAC CCCATCAGCT 
ATGGCTTCTA TTCCTGCCCT TCCTCAAACA GCAGGACCTA CTTCTACACC TTTATTGCCC 
CAAGTACTTA GTATCCCACC CTTGGTACAA CCTGTTGCCA GTGTGCCTGC TGTGCAGCAG 
ACACTAATTC ATAGTCAGCC TCAACCAGCT TTACTTCCCA ACCAGCCCCA CACTCACTGT 
CCTGAAATAG ATGCTGACTT ACAGCCCAAA GCTCCTGGAA TTGATGACAT AAAGACTCTA 
GAGGAAAAGC TGCGGTCTCT CTTTAGTGAA CCGAGCTCAT CTGGAGCCCA ACATACATCG 
GTCTCACTAG AGACTTCACT GGTAGTAGAG ACCACTGTCA CGCCAGGTAT CCCAACCACT 
GCTATTGCAC CAAGCAAGCT CATGACATCT ACTACAAGTA CATGCTTACC ACCAACCAGT 
TTGCAATTAG GAACATCTGG TTTGTCAGTT ATACCAGTGG TCACGCCTGG GCAGGTTTCT 
ACCCCAGTCA GCTATGTGTC AGCCCCAGGC AGCACTACAT CAGGAGTGAA AGCTGGAACT 
GCTCCATCAA AGCCACCTTT GACTAAAGCT CCAGTGCTGC CAGTGGGTAC TGAACTTCCA 
GCTGGTACTC CACCCAGCGA GCAGCTGCCA CCTTTTCCAG GACCTTCACT AACTCAGTCC 
CAGCAACCTC TGGAAGATCT TGATGCTCAG TTGAGAAGAA CACTTAGTCC AGAGACTATT 
GCGGTGACAT CTACAATTGG TCCTGTGTCC ATGGTGGCTC CAACAGCAGT CACAGAAGCA 
GGAGCACAGC CTCAGAAGGA TGTTTCTCAG ATCACAGAAG GTCCTGTCCT AGCGACTAGT 
TCGGGAACTG GTGTTTTTAA GATGGGAAGA TTTCAGGTTT CAGTTGCAAT GGATGATGTT 
CAAAAGGAGG GTAAAAATAA GTTAGAAGAT GGAAAGTCTG TTCATTTTGA ATCCAGCACT 
TCAGAGTCCT CAGTGCTATC AAGTAGTAGT CCAGAAAGTA CCCTGGTGAA GACAGAGCCT 
AATGGGATAA CCATCCCTGG CATCTCATCA GATATGCCAG ATAGTGCCCA CAAAACTCCT 
GCCTCAGAAG CAAAGTCAGA AACTGGGCAG CCTACCAAGG TTGGACGTTT TCAGGTGACA 
ACTACAGCAA ACAAAGTAGG TCGTTTCTCT GTGTCAAGAA CTGAAGACAA GCTCATTGAG 
GCAAAGAAAG AGGGACCAAT AGCGTCTCCT CCTTTTATGG ATTTGGAACA AGGTGTTCTT 
CCAGCTGTGA TACCAAAGAA AGAAAAGCCT GAACTGTCAG AGCCTTCACA TCTGAACGGA 
CCATCTTCTG ACCTGGAGGC TGCCTTCCTG AGCAGGGATG TGGATGATGG TTCAGGTAGT 
CCACACTCCC CCCATCAGCT GAGCTCAAAG AGCCTGCCTA TCCAGAATCT AAGTCAAAGC 
CTTAGTAATT CATTCAACTC CTCTTACATG AGTAGTGACA ATGAGTCAGA TATTGAAGAT 
GAAGATTTAA AATTAGAGCT GCGAAGACTA CGAGAAAAAC ATCTTAAAGA GATTCAAGAC 
CTGCAGAGTC GCCAGAAGCA TGAAATTGAA TCTTTGTATA CCAAACTGGG AAAGGTTCCC 
CCTGCTGTCA TTATCCCCCC AGCTGCCCCC CTTTCAGGGA GAAGAAGGCG ACCCACTAAA 
AGCAAAGGCA GCAAGTCTAG TCGCAGCAGT TCCTTGGGGA ATAAAAGTCC CCAGCTTTCA 
GGTAACCTGT CTGGTCAGAG TGCTGCTTCA GTTTTGCACG CTCAGCAGAC CCTCCACCCT 
CCTGGCAACA TCCCTGAGAC TGGGCCAAAT CAGCTGTTAC AGCCCCTTAA GCCATCTCCC 
TCCAGTGACA ACCTCTACTC AGCCTTTACC AGTGATGGTG CCATTTCAGT CCCAAGCCTT 
TCTGCTCCAG GTCAAGGCTG TGCGAAGTTT AACTGTGCAT CTGAGCAGGT GACATTCAAA 
CCTGGTGGCA GGAGGACCCG ATTTCTGAGT ACGCCCTGCT TGGCTCTTTG GAAGATGGTG 
AAAAAAGTCT GTCCTTGCAA CCAGCTCTGT ATGTGCCCTC CAGCAGAGCC GAAATCAGGG 
ACCAGCAGCA CAAACACTGT CGGGGGAACA GTGAACAGCC AAGCTGCCCC AGCTCAGCCT 
CCTGCCATGA CATCCAGCAG GAAGGGCACA TTCACAGATG ACCTACACAA GTTGGTAGAC 
AATTGGGCCC GAGATGCCAT GAATCTCTCA GGCAGAAGAG GAAGCAAAGG ACACATGAAT 
TATGAGGGCC CTGGAATGGC AAGAAAGTTC TCCGCACCTG GTCAGCTGTG CATCTCCATG 
ACCTCAAATC TGGGTGGTTC TGCCCCCATC TCTGCAGCAT CAGCTACCTC TCTAGGTCAC 
TTCACCAAGT CTATGTGCCC CCCACAGCAG TACGGTTTTC CAGCTCCCCC CTTTGGCACT 
CAGTGGAGTG GGACGGGTGG CCCGGCACCA CAGCCACTTG GCCAGTTCCA GCCTGTGGGA 
ACTGCCTCCT TACAGAATTT CAACATCAGC AATTTGCAGA AATCCATCAG CAACCCCCCA 
GGTTCCAACC TGCGGACCAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963815.1
CDS	99..6962
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVGQSQGQPSSSSLTGIPSSLPIQHSQQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQGFPSRLPPQYPGDSNIAPSSSVASICIPSTVLSPPMPTEALATPGYFPTVVQPFVESNLLVPVGSIGGQVQVSQPAVSLAQQAPTTSSQQAALESTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGCAKFNCASEQVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCMCPPAEPKSGTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_014108340.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108340.1

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGGG AGTACAGCAG ACAGCCCCTC CACAACAGAC AGCGCAGTAT 
TCGCTTCCAC AGACATCAGC CTCCAGTGAG GCCACTATTG CACAACCAGT CAGTCAGTCT 
CAGGCTCCAC AGGTCTTGCC TCAAATATCA GCTGGAAAAC AGGGCTTTCC ATCTCGACTG 
CCACCACAGT ACCCAGGAGA TTCAAATATT GCTCCTTCTT CCAGCGTGGC TTCTATTTGC 
ATCCCTTCTA CAGTCCTATC CCCTCCCATG CCCACAGAAG CACTGGCTAC ACCGGGTTAC 
TTTCCTACAG TGGTGCAGCC TTTTGTGGAA TCTAACCTTT TGGTTCCTGT GGGCAGTATA 
GGAGGACAGG TTCAAGTGTC CCAGCCAGCA GTGAGTTTAG CACAACAAGC CCCCACTACA 
TCCTCCCAGC AAGCAGCTCT GGAGAGCACT CAGGGAGTCT CTCAAGTTGC TCCTCCAGAG 
CCAGTTCCAG TGGCACAGGC ACAGCCTACC ACTTTGGTCT CCTCTATAGA CAGTGCACAT 
TCAGATGTTG CTTCAGGAAT GAGTGATGGC AATGAGAATG TCCCGTCCTC CAGTGGAAGG 
CATGAAGGGA GAGCTACAAA ACGACATTAC CGAAAATCTG TAAGAAGTCG CTCTCGTCAT 
GAAAAGACTT CGCGTCCAAA ATTAAGAATT TTGAATGTTT CAAATAAAGG AGACCGAGTA 
GTAGAATGTC AACTAGAGAC TCATAATCGG AAAATGGTTA CATTCAAATT TGATTTAGAT 
GGTGACAATC CTGAGGAGAT AGCAACAATT ATGGTGAACA ATGACTTTAT TCTAGCAATG 
GAGAGAGAGT CCTTTGTGGA TCAAGTACGA GAAATTATTG AAAAGGCTGA TGAAATGCTC 
AGTGAGGATG TCAGTGTGGA ACCAGAGGGT GACCAGGGAT TGGAGAGTCT GCAGGGAAAG 
GATGACTATG GCTTTACAGG TTCTCAAAAA TTGGAAGGAG AATTCAAACA ACCAGTTCCT 
GCGTCTTCCA TGCCACAGCA AATAGGCATT CCTACCAGTT CTTTAACTCA AGTTGTTCAT 
TCTGCTGGAA GACGGTTTAT AGTGAGTCCT GTGCCAGAAA GTCGATTACG AGAATCAAAA 
GTTTTCACTA GTGAAATACC AGATACAGTT GCTGCCTCTA CTCACAGCGC TGGAATGAAC 
TTGTCTCACT CTGCTTCATC CCTTAGCCTA CAGCAAGCCT TTTCTGAACT TAGACATGTG 
CAGATGACAG AAGGGCCCAG CACAGCACCT CCCAACTTCA GTCATACAGG ACCAACATTT 
CCAGTTGTTC CTCCTTCCAT GAGTAGCATT GCTGGAGTCC CAACCACAGC AGTAGCCACA 
CCTTCAGTAT CAGTCCCTGC AACTAGCAGC CCTCTAAATG ACAGTTCCAC ATCAGTAATT 
CAATCTGAGA TTCCAGTGCC CACTGAAACA GGGGTTGGTG GAGTTCCCAC CTGCACAAGT 
GTAATGCCTT CAAGTGGTCT CCCTGTACCA CCCATATCTG AATCGCCAAT ATTTTCCACT 
ATGGTTTCAA GCATCACAGT ACCTACAGTT GTCTCAATAT CAGCAACATC CCAGCCAGTT 
CAAGCTCCCA CATCTGGGAG CACTGTTTCT AGCATAGGAA CTTTGCCTTC TATACCAGTT 
TCAACAGCTT TAGCCTCTGC AGTGGGCAGT GTTGCTATCC CAGGTGCTAA GCCTCCACCT 
GTACTATCAC AGCAGGCAGC AGGCAGTACT ACTGGGGCAG CCACATTAAC ATCCGTTCCT 
GCCATCACTC CGTTCCCAAG CATAGCTTCA CAGCCATCTC TTCAGCTTAG CAGCAGCACC 
TCTGCTCCAA CTCTAGCTGA AACAGTGGTG GTTAGTGCAC ACTCACTAGA TAAAACATCT 
CATAGCAGTA CAACTGGATT GGCTTTGTCC CTGTCTGCAT CATCTTCCTC CCCTGGAGCA 
GGATTATCTA GTTCTATTTC TCAGCCTGGT GGGGTGCATC CTTTGGTTAC CCCATCAGCT 
ATGGCTTCTA TTCCTGCCCT TCCTCAAACA GCAGGACCTA CTTCTACACC TTTATTGCCC 
CAAGTACTTA GTATCCCACC CTTGGTACAA CCTGTTGCCA GTGTGCCTGC TGTGCAGCAG 
ACACTAATTC ATAGTCAGCC TCAACCAGCT TTACTTCCCA ACCAGCCCCA CACTCACTGT 
CCTGAAATAG ATGCTGACTT ACAGCCCAAA GCTCCTGGAA TTGATGACAT AAAGACTCTA 
GAGGAAAAGC TGCGGTCTCT CTTTAGTGAA CCGAGCTCAT CTGGAGCCCA ACATACATCG 
GTCTCACTAG AGACTTCACT GGTAGTAGAG ACCACTGTCA CGCCAGGTAT CCCAACCACT 
GCTATTGCAC CAAGCAAGCT CATGACATCT ACTACAAGTA CATGCTTACC ACCAACCAGT 
TTGCAATTAG GAACATCTGG TTTGTCAGTT ATACCAGTGG TCACGCCTGG GCAGGTTTCT 
ACCCCAGTCA GCTATGTGTC AGCCCCAGGC AGCACTACAT CAGGAGTGAA AGCTGGAACT 
GCTCCATCAA AGCCACCTTT GACTAAAGCT CCAGTGCTGC CAGTGGGTAC TGAACTTCCA 
GCTGGTACTC CACCCAGCGA GCAGCTGCCA CCTTTTCCAG GACCTTCACT AACTCAGTCC 
CAGCAACCTC TGGAAGATCT TGATGCTCAG TTGAGAAGAA CACTTAGTCC AGAGACTATT 
GCGGTGACAT CTACAATTGG TCCTGTGTCC ATGGTGGCTC CAACAGCAGT CACAGAAGCA 
GGAGCACAGC CTCAGAAGGA TGTTTCTCAG ATCACAGAAG GTCCTGTCCT AGCGACTAGT 
TCGGGAACTG GTGTTTTTAA GATGGGAAGA TTTCAGGTTT CAGTTGCAAT GGATGATGTT 
CAAAAGGAGG GTAAAAATAA GTTAGAAGAT GGAAAGTCTG TTCATTTTGA ATCCAGCACT 
TCAGAGTCCT CAGTGCTATC AAGTAGTAGT CCAGAAAGTA CCCTGGTGAA GACAGAGCCT 
AATGGGATAA CCATCCCTGG CATCTCATCA GATATGCCAG ATAGTGCCCA CAAAACTCCT 
GCCTCAGAAG CAAAGTCAGA AACTGGGCAG CCTACCAAGG TTGGACGTTT TCAGGTGACA 
ACTACAGCAA ACAAAGTAGG TCGTTTCTCT GTGTCAAGAA CTGAAGACAA GCTCATTGAG 
GCAAAGAAAG AGGGACCAAT AGCGTCTCCT CCTTTTATGG ATTTGGAACA AGGTGTTCTT 
CCAGCTGTGA TACCAAAGAA AGAAAAGCCT GAACTGTCAG AGCCTTCACA TCTGAACGGA 
CCATCTTCTG ACCTGGAGGC TGCCTTCCTG AGCAGGGATG TGGATGATGG TTCAGGTAGT 
CCACACTCCC CCCATCAGCT GAGCTCAAAG AGCCTGCCTA TCCAGAATCT AAGTCAAAGC 
CTTAGTAATT CATTCAACTC CTCTTACATG AGTAGTGACA ATGAGTCAGA TATTGAAGAT 
GAAGATTTAA AATTAGAGCT GCGAAGACTA CGAGAAAAAC ATCTTAAAGA GATTCAAGAC 
CTGCAGAGTC GCCAGAAGCA TGAAATTGAA TCTTTGTATA CCAAACTGGG AAAGGTTCCC 
CCTGCTGTCA TTATCCCCCC AGCTGCCCCC CTTTCAGGGA GAAGAAGGCG ACCCACTAAA 
AGCAAAGGCA GCAAGTCTAG TCGCAGCAGT TCCTTGGGGA ATAAAAGTCC CCAGCTTTCA 
GGTAACCTGT CTGGTCAGAG TGCTGCTTCA GTTTTGCACG CTCAGCAGAC CCTCCACCCT 
CCTGGCAACA TCCCTGAGAC TGGGCCAAAT CAGCTGTTAC AGCCCCTTAA GCCATCTCCC 
TCCAGTGACA ACCTCTACTC AGCCTTTACC AGTGATGGTG CCATTTCAGT CCCAAGCCTT 
TCTGCTCCAG GTCAAGGCTG TGCGAAGTTT AACTGTGCAT CTGAGCAGGT GACATTCAAA 
CCTGGTGGCA GGAGGACCCG ATTTCTGAGT ACGCCCTGCT TGGCTCTTTG GAAGATGGTG 
AAAAAAGTCT GTCCTTGCAA CCAGCTCTGT ATGTGCCCTC CAGCAGAGCC GAAATCAGGG 
ACCAGCAGCA CAAACACTGT CGGGGGAACA GTGAACAGCC AAGCTGCCCC AGCTCAGCCT 
CCTGCCATGA CATCCAGCAG GAAGGGCACA TTCACAGATG ACCTACACAA GTTGGTAGAC 
AATTGGGCCC GAGATGCCAT GAATCTCTCA GGCAGAAGAG GAAGCAAAGG ACACATGAAT 
TATGAGGGCC CTGGAATGGC AAGAAAGTTC TCCGCACCTG GTCAGCTGTG CATCTCCATG 
ACCTCAAATC TGGGTGGTTC TGCCCCCATC TCTGCAGCAT CAGCTACCTC TCTAGGTCAC 
TTCACCAAGT CTATGTGCCC CCCACAGCAG TACGGTTTTC CAGCTCCCCC CTTTGGCACT 
CAGTGGAGTG GGACGGGTGG CCCGGCACCA CAGCCACTTG GCCAGTTCCA GCCTGTGGGA 
ACTGCCTCCT TACAGAATTT CAACATCAGC AATTTGCAGA AATCCATCAG CAACCCCCCA 
GGTTCCAACC TGCGGACCAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_014108341.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963816.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X15, mRNA.

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CloneID	OCe43585
Clone ID Related Accession (Same CDS sequence)	XM_014108341.2 , XM_014108341.1
Accession Version	XM_014108341.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase WNK1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014108341.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGGGCTT TCCATCTCGA 
CTGCCACCAC AGTACCCAGG AGATTCAAAT ATTGCTCCTT CTTCCAGCGT GGCTTCTATT 
TGCATCCCTT CTACAGTCCT ATCCCCTCCC ATGCCCACAG AAGCACTGGC TACACCGGGT 
TACTTTCCTA CAGTGGTGCA GCCTTTTGTG GAATCTAACC TTTTGGTTCC TGTGGGCAGT 
ATAGGAGGAC AGGTTCAAGT GTCCCAGCCA GCAGTGAGTT TAGCACAACA AGCCCCCACT 
ACATCCTCCC AGCAAGCAGC TCTGGAGAGC ACTCAGGGAG TCTCTCAAGT TGCTCCTCCA 
GAGCCAGTTC CAGTGGCACA GGCACAGCCT ACCACTTTGG TCTCCTCTAT AGACAGTGCA 
CATTCAGATG TTGCTTCAGG AATGAGTGAT GGCAATGAGA ATGTCCCGTC CTCCAGTGGA 
AGGCATGAAG GGAGAGCTAC AAAACGACAT TACCGAAAAT CTGTAAGAAG TCGCTCTCGT 
CATGAAAAGA CTTCGCGTCC AAAATTAAGA ATTTTGAATG TTTCAAATAA AGGAGACCGA 
GTAGTAGAAT GTCAACTAGA GACTCATAAT CGGAAAATGG TTACATTCAA ATTTGATTTA 
GATGGTGACA ATCCTGAGGA GATAGCAACA ATTATGGTGA ACAATGACTT TATTCTAGCA 
ATGGAGAGAG AGTCCTTTGT GGATCAAGTA CGAGAAATTA TTGAAAAGGC TGATGAAATG 
CTCAGTGAGG ATGTCAGTGT GGAACCAGAG GGTGACCAGG GATTGGAGAG TCTGCAGGGA 
AAGGATGACT ATGGCTTTAC AGGTTCTCAA AAATTGGAAG GAGAATTCAA ACAACCAGTT 
CCTGCGTCTT CCATGCCACA GCAAATAGGC ATTCCTACCA GTTCTTTAAC TCAAGTTGTT 
CATTCTGCTG GAAGACGGTT TATAGTGAGT CCTGTGCCAG AAAGTCGATT ACGAGAATCA 
AAAGTTTTCA CTAGTGAAAT ACCAGATACA GTTGCTGCCT CTACTCACAG CGCTGGAATG 
AACTTGTCTC ACTCTGCTTC ATCCCTTAGC CTACAGCAAG CCTTTTCTGA ACTTAGACAT 
GTGCAGATGA CAGAAGGGCC CAGCACAGCA CCTCCCAACT TCAGTCATAC AGGACCAACA 
TTTCCAGTTG TTCCTCCTTC CATGAGTAGC ATTGCTGGAG TCCCAACCAC AGCAGTAGCC 
ACACCTTCAG TATCAGTCCC TGCAACTAGC AGCCCTCTAA ATGACAGTTC CACATCAGTA 
ATTCAATCTG AGATTCCAGT GCCCACTGAA ACAGGGGTTG GTGGAGTTCC CACCTGCACA 
AGTGTAATGC CTTCAAGTGG TCTCCCTGTA CCACCCATAT CTGAATCGCC AATATTTTCC 
ACTATGGTTT CAAGCATCAC AGTACCTACA GTTGTCTCAA TATCAGCAAC ATCCCAGCCA 
GTTCAAGCTC CCACATCTGG GAGCACTGTT TCTAGCATAG GAACTTTGCC TTCTATACCA 
GTTTCAACAG CTTTAGCCTC TGCAGTGGGC AGTGTTGCTA TCCCAGGTGC TAAGCCTCCA 
CCTGTACTAT CACAGCAGGC AGCAGGCAGT ACTACTGGGG CAGCCACATT AACATCCGTT 
CCTGCCATCA CTCCGTTCCC AAGCATAGCT TCACAGCCAT CTCTTCAGCT TAGCAGCAGC 
ACCTCTGCTC CAACTCTAGC TGAAACAGTG GTGGTTAGTG CACACTCACT AGATAAAACA 
TCTCATAGCA GTACAACTGG ATTGGCTTTG TCCCTGTCTG CATCATCTTC CTCCCCTGGA 
GCAGGATTAT CTAGTTCTAT TTCTCAGCCT GGTGGGGTGC ATCCTTTGGT TACCCCATCA 
GCTATGGCTT CTATTCCTGC CCTTCCTCAA ACAGCAGGAC CTACTTCTAC ACCTTTATTG 
CCCCAAGTAC TTAGTATCCC ACCCTTGGTA CAACCTGTTG CCAGTGTGCC TGCTGTGCAG 
CAGACACTAA TTCATAGTCA GCCTCAACCA GCTTTACTTC CCAACCAGCC CCACACTCAC 
TGTCCTGAAA TAGATGCTGA CTTACAGCCC AAAGCTCCTG GAATTGATGA CATAAAGACT 
CTAGAGGAAA AGCTGCGGTC TCTCTTTAGT GAACCGAGCT CATCTGGAGC CCAACATACA 
TCGGTCTCAC TAGAGACTTC ACTGGTAGTA GAGACCACTG TCACGCCAGG TATCCCAACC 
ACTGCTATTG CACCAAGCAA GCTCATGACA TCTACTACAA GTACATGCTT ACCACCAACC 
AGTTTGCAAT TAGGAACATC TGGTTTGTCA GTTATACCAG TGGTCACGCC TGGGCAGGTT 
TCTACCCCAG TCAGCTATGT GTCAGCCCCA GGCAGCACTA CATCAGGAGT GAAAGCTGGA 
ACTGCTCCAT CAAAGCCACC TTTGACTAAA GCTCCAGTGC TGCCAGTGGG TACTGAACTT 
CCAGCTGGTA CTCCACCCAG CGAGCAGCTG CCACCTTTTC CAGGACCTTC ACTAACTCAG 
TCCCAGCAAC CTCTGGAAGA TCTTGATGCT CAGTTGAGAA GAACACTTAG TCCAGAGACT 
ATTGCGGTGA CATCTACAAT TGGTCCTGTG TCCATGGTGG CTCCAACAGC AGTCACAGAA 
GCAGGAGCAC AGCCTCAGAA GGATGTTTCT CAGATCACAG AAGGTCCTGT CCTAGCGACT 
AGTTCGGGAA CTGGTGTTTT TAAGATGGGA AGATTTCAGG TTTCAGTTGC AATGGATGAT 
GTTCAAAAGG AGGGTAAAAA TAAGTTAGAA GATGGAAAGT CTGTTCATTT TGAATCCAGC 
ACTTCAGAGT CCTCAGTGCT ATCAAGTAGT AGTCCAGAAA GTACCCTGGT GAAGACAGAG 
CCTAATGGGA TAACCATCCC TGGCATCTCA TCAGATATGC CAGATAGTGC CCACAAAACT 
CCTGCCTCAG AAGCAAAGTC AGAAACTGGG CAGCCTACCA AGGTTGGACG TTTTCAGGTG 
ACAACTACAG CAAACAAAGT AGGTCGTTTC TCTGTGTCAA GAACTGAAGA CAAGCTCATT 
GAGGCAAAGA AAGAGGGACC AATAGCGTCT CCTCCTTTTA TGGATTTGGA ACAAGGTGTT 
CTTCCAGCTG TGATACCAAA GAAAGAAAAG CCTGAACTGT CAGAGCCTTC ACATCTGAAC 
GGACCATCTT CTGACCTGGA GGCTGCCTTC CTGAGCAGGG ATGTGGATGA TGGTTCAGGT 
AGTCCACACT CCCCCCATCA GCTGAGCTCA AAGAGCCTGC CTATCCAGAA TCTAAGTCAA 
AGCCTTAGTA ATTCATTCAA CTCCTCTTAC ATGAGTAGTG ACAATGAGTC AGATATTGAA 
GATGAAGATT TAAAATTAGA GCTGCGAAGA CTACGAGAAA AACATCTTAA AGAGATTCAA 
GACCTGCAGA GTCGCCAGAA GCATGAAATT GAATCTTTGT ATACCAAACT GGGAAAGGTT 
CCCCCTGCTG TCATTATCCC CCCAGCTGCC CCCCTTTCAG GGAGAAGAAG GCGACCCACT 
AAAAGCAAAG GCAGCAAGTC TAGTCGCAGC AGTTCCTTGG GGAATAAAAG TCCCCAGCTT 
TCAGGTAACC TGTCTGGTCA GAGTGCTGCT TCAGTTTTGC ACGCTCAGCA GACCCTCCAC 
CCTCCTGGCA ACATCCCTGA GACTGGGCCA AATCAGCTGT TACAGCCCCT TAAGCCATCT 
CCCTCCAGTG ACAACCTCTA CTCAGCCTTT ACCAGTGATG GTGCCATTTC AGTCCCAAGC 
CTTTCTGCTC CAGGTCAAGG GACCAGCAGC ACAAACACTG TCGGGGGAAC AGTGAACAGC 
CAAGCTGCCC CAGCTCAGCC TCCTGCCATG ACATCCAGCA GGAAGGGCAC ATTCACAGAT 
GACCTACACA AGTTGGTAGA CAATTGGGCC CGAGATGCCA TGAATCTCTC AGGCAGAAGA 
GGAAGCAAAG GACACATGAA TTATGAGGGC CCTGGAATGG CAAGAAAGTT CTCCGCACCT 
GGTCAGCTGT GCATCTCCAT GACCTCAAAT CTGGGTGGTT CTGCCCCCAT CTCTGCAGCA 
TCAGCTACCT CTCTAGGTCA CTTCACCAAG TCTATGTGCC CCCCACAGCA GTACGGTTTT 
CCAGCTCCCC CCTTTGGCAC TCAGTGGAGT GGGACGGGTG GCCCGGCACC ACAGCCACTT 
GGCCAGTTCC AGCCTGTGGG AACTGCCTCC TTACAGAATT TCAACATCAG CAATTTGCAG 
AAATCCATCA GCAACCCCCC AGGTTCCAAC CTGCGGACCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963816.1
CDS	995..7699
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVGQSQGQPSSSSLTGIPSSLPIQHSQQQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQGFPSRLPPQYPGDSNIAPSSSVASICIPSTVLSPPMPTEALATPGYFPTVVQPFVESNLLVPVGSIGGQVQVSQPAVSLAQQAPTTSSQQAALESTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_014108341.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108341.2

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGGGCTT TCCATCTCGA 
CTGCCACCAC AGTACCCAGG AGATTCAAAT ATTGCTCCTT CTTCCAGCGT GGCTTCTATT 
TGCATCCCTT CTACAGTCCT ATCCCCTCCC ATGCCCACAG AAGCACTGGC TACACCGGGT 
TACTTTCCTA CAGTGGTGCA GCCTTTTGTG GAATCTAACC TTTTGGTTCC TGTGGGCAGT 
ATAGGAGGAC AGGTTCAAGT GTCCCAGCCA GCAGTGAGTT TAGCACAACA AGCCCCCACT 
ACATCCTCCC AGCAAGCAGC TCTGGAGAGC ACTCAGGGAG TCTCTCAAGT TGCTCCTCCA 
GAGCCAGTTC CAGTGGCACA GGCACAGCCT ACCACTTTGG TCTCCTCTAT AGACAGTGCA 
CATTCAGATG TTGCTTCAGG AATGAGTGAT GGCAATGAGA ATGTCCCGTC CTCCAGTGGA 
AGGCATGAAG GGAGAGCTAC AAAACGACAT TACCGAAAAT CTGTAAGAAG TCGCTCTCGT 
CATGAAAAGA CTTCGCGTCC AAAATTAAGA ATTTTGAATG TTTCAAATAA AGGAGACCGA 
GTAGTAGAAT GTCAACTAGA GACTCATAAT CGGAAAATGG TTACATTCAA ATTTGATTTA 
GATGGTGACA ATCCTGAGGA GATAGCAACA ATTATGGTGA ACAATGACTT TATTCTAGCA 
ATGGAGAGAG AGTCCTTTGT GGATCAAGTA CGAGAAATTA TTGAAAAGGC TGATGAAATG 
CTCAGTGAGG ATGTCAGTGT GGAACCAGAG GGTGACCAGG GATTGGAGAG TCTGCAGGGA 
AAGGATGACT ATGGCTTTAC AGGTTCTCAA AAATTGGAAG GAGAATTCAA ACAACCAGTT 
CCTGCGTCTT CCATGCCACA GCAAATAGGC ATTCCTACCA GTTCTTTAAC TCAAGTTGTT 
CATTCTGCTG GAAGACGGTT TATAGTGAGT CCTGTGCCAG AAAGTCGATT ACGAGAATCA 
AAAGTTTTCA CTAGTGAAAT ACCAGATACA GTTGCTGCCT CTACTCACAG CGCTGGAATG 
AACTTGTCTC ACTCTGCTTC ATCCCTTAGC CTACAGCAAG CCTTTTCTGA ACTTAGACAT 
GTGCAGATGA CAGAAGGGCC CAGCACAGCA CCTCCCAACT TCAGTCATAC AGGACCAACA 
TTTCCAGTTG TTCCTCCTTC CATGAGTAGC ATTGCTGGAG TCCCAACCAC AGCAGTAGCC 
ACACCTTCAG TATCAGTCCC TGCAACTAGC AGCCCTCTAA ATGACAGTTC CACATCAGTA 
ATTCAATCTG AGATTCCAGT GCCCACTGAA ACAGGGGTTG GTGGAGTTCC CACCTGCACA 
AGTGTAATGC CTTCAAGTGG TCTCCCTGTA CCACCCATAT CTGAATCGCC AATATTTTCC 
ACTATGGTTT CAAGCATCAC AGTACCTACA GTTGTCTCAA TATCAGCAAC ATCCCAGCCA 
GTTCAAGCTC CCACATCTGG GAGCACTGTT TCTAGCATAG GAACTTTGCC TTCTATACCA 
GTTTCAACAG CTTTAGCCTC TGCAGTGGGC AGTGTTGCTA TCCCAGGTGC TAAGCCTCCA 
CCTGTACTAT CACAGCAGGC AGCAGGCAGT ACTACTGGGG CAGCCACATT AACATCCGTT 
CCTGCCATCA CTCCGTTCCC AAGCATAGCT TCACAGCCAT CTCTTCAGCT TAGCAGCAGC 
ACCTCTGCTC CAACTCTAGC TGAAACAGTG GTGGTTAGTG CACACTCACT AGATAAAACA 
TCTCATAGCA GTACAACTGG ATTGGCTTTG TCCCTGTCTG CATCATCTTC CTCCCCTGGA 
GCAGGATTAT CTAGTTCTAT TTCTCAGCCT GGTGGGGTGC ATCCTTTGGT TACCCCATCA 
GCTATGGCTT CTATTCCTGC CCTTCCTCAA ACAGCAGGAC CTACTTCTAC ACCTTTATTG 
CCCCAAGTAC TTAGTATCCC ACCCTTGGTA CAACCTGTTG CCAGTGTGCC TGCTGTGCAG 
CAGACACTAA TTCATAGTCA GCCTCAACCA GCTTTACTTC CCAACCAGCC CCACACTCAC 
TGTCCTGAAA TAGATGCTGA CTTACAGCCC AAAGCTCCTG GAATTGATGA CATAAAGACT 
CTAGAGGAAA AGCTGCGGTC TCTCTTTAGT GAACCGAGCT CATCTGGAGC CCAACATACA 
TCGGTCTCAC TAGAGACTTC ACTGGTAGTA GAGACCACTG TCACGCCAGG TATCCCAACC 
ACTGCTATTG CACCAAGCAA GCTCATGACA TCTACTACAA GTACATGCTT ACCACCAACC 
AGTTTGCAAT TAGGAACATC TGGTTTGTCA GTTATACCAG TGGTCACGCC TGGGCAGGTT 
TCTACCCCAG TCAGCTATGT GTCAGCCCCA GGCAGCACTA CATCAGGAGT GAAAGCTGGA 
ACTGCTCCAT CAAAGCCACC TTTGACTAAA GCTCCAGTGC TGCCAGTGGG TACTGAACTT 
CCAGCTGGTA CTCCACCCAG CGAGCAGCTG CCACCTTTTC CAGGACCTTC ACTAACTCAG 
TCCCAGCAAC CTCTGGAAGA TCTTGATGCT CAGTTGAGAA GAACACTTAG TCCAGAGACT 
ATTGCGGTGA CATCTACAAT TGGTCCTGTG TCCATGGTGG CTCCAACAGC AGTCACAGAA 
GCAGGAGCAC AGCCTCAGAA GGATGTTTCT CAGATCACAG AAGGTCCTGT CCTAGCGACT 
AGTTCGGGAA CTGGTGTTTT TAAGATGGGA AGATTTCAGG TTTCAGTTGC AATGGATGAT 
GTTCAAAAGG AGGGTAAAAA TAAGTTAGAA GATGGAAAGT CTGTTCATTT TGAATCCAGC 
ACTTCAGAGT CCTCAGTGCT ATCAAGTAGT AGTCCAGAAA GTACCCTGGT GAAGACAGAG 
CCTAATGGGA TAACCATCCC TGGCATCTCA TCAGATATGC CAGATAGTGC CCACAAAACT 
CCTGCCTCAG AAGCAAAGTC AGAAACTGGG CAGCCTACCA AGGTTGGACG TTTTCAGGTG 
ACAACTACAG CAAACAAAGT AGGTCGTTTC TCTGTGTCAA GAACTGAAGA CAAGCTCATT 
GAGGCAAAGA AAGAGGGACC AATAGCGTCT CCTCCTTTTA TGGATTTGGA ACAAGGTGTT 
CTTCCAGCTG TGATACCAAA GAAAGAAAAG CCTGAACTGT CAGAGCCTTC ACATCTGAAC 
GGACCATCTT CTGACCTGGA GGCTGCCTTC CTGAGCAGGG ATGTGGATGA TGGTTCAGGT 
AGTCCACACT CCCCCCATCA GCTGAGCTCA AAGAGCCTGC CTATCCAGAA TCTAAGTCAA 
AGCCTTAGTA ATTCATTCAA CTCCTCTTAC ATGAGTAGTG ACAATGAGTC AGATATTGAA 
GATGAAGATT TAAAATTAGA GCTGCGAAGA CTACGAGAAA AACATCTTAA AGAGATTCAA 
GACCTGCAGA GTCGCCAGAA GCATGAAATT GAATCTTTGT ATACCAAACT GGGAAAGGTT 
CCCCCTGCTG TCATTATCCC CCCAGCTGCC CCCCTTTCAG GGAGAAGAAG GCGACCCACT 
AAAAGCAAAG GCAGCAAGTC TAGTCGCAGC AGTTCCTTGG GGAATAAAAG TCCCCAGCTT 
TCAGGTAACC TGTCTGGTCA GAGTGCTGCT TCAGTTTTGC ACGCTCAGCA GACCCTCCAC 
CCTCCTGGCA ACATCCCTGA GACTGGGCCA AATCAGCTGT TACAGCCCCT TAAGCCATCT 
CCCTCCAGTG ACAACCTCTA CTCAGCCTTT ACCAGTGATG GTGCCATTTC AGTCCCAAGC 
CTTTCTGCTC CAGGTCAAGG GACCAGCAGC ACAAACACTG TCGGGGGAAC AGTGAACAGC 
CAAGCTGCCC CAGCTCAGCC TCCTGCCATG ACATCCAGCA GGAAGGGCAC ATTCACAGAT 
GACCTACACA AGTTGGTAGA CAATTGGGCC CGAGATGCCA TGAATCTCTC AGGCAGAAGA 
GGAAGCAAAG GACACATGAA TTATGAGGGC CCTGGAATGG CAAGAAAGTT CTCCGCACCT 
GGTCAGCTGT GCATCTCCAT GACCTCAAAT CTGGGTGGTT CTGCCCCCAT CTCTGCAGCA 
TCAGCTACCT CTCTAGGTCA CTTCACCAAG TCTATGTGCC CCCCACAGCA GTACGGTTTT 
CCAGCTCCCC CCTTTGGCAC TCAGTGGAGT GGGACGGGTG GCCCGGCACC ACAGCCACTT 
GGCCAGTTCC AGCCTGTGGG AACTGCCTCC TTACAGAATT TCAACATCAG CAATTTGCAG 
AAATCCATCA GCAACCCCCC AGGTTCCAAC CTGCGGACCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_014108341.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963816.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X17, mRNA.

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CloneID	OCe43585
Clone ID Related Accession (Same CDS sequence)	XM_014108341.2 , XM_014108341.1
Accession Version	XM_014108341.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase WNK1 isoform X17
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGGGCTT TCCATCTCGA 
CTGCCACCAC AGTACCCAGG AGATTCAAAT ATTGCTCCTT CTTCCAGCGT GGCTTCTATT 
TGCATCCCTT CTACAGTCCT ATCCCCTCCC ATGCCCACAG AAGCACTGGC TACACCGGGT 
TACTTTCCTA CAGTGGTGCA GCCTTTTGTG GAATCTAACC TTTTGGTTCC TGTGGGCAGT 
ATAGGAGGAC AGGTTCAAGT GTCCCAGCCA GCAGTGAGTT TAGCACAACA AGCCCCCACT 
ACATCCTCCC AGCAAGCAGC TCTGGAGAGC ACTCAGGGAG TCTCTCAAGT TGCTCCTCCA 
GAGCCAGTTC CAGTGGCACA GGCACAGCCT ACCACTTTGG TCTCCTCTAT AGACAGTGCA 
CATTCAGATG TTGCTTCAGG AATGAGTGAT GGCAATGAGA ATGTCCCGTC CTCCAGTGGA 
AGGCATGAAG GGAGAGCTAC AAAACGACAT TACCGAAAAT CTGTAAGAAG TCGCTCTCGT 
CATGAAAAGA CTTCGCGTCC AAAATTAAGA ATTTTGAATG TTTCAAATAA AGGAGACCGA 
GTAGTAGAAT GTCAACTAGA GACTCATAAT CGGAAAATGG TTACATTCAA ATTTGATTTA 
GATGGTGACA ATCCTGAGGA GATAGCAACA ATTATGGTGA ACAATGACTT TATTCTAGCA 
ATGGAGAGAG AGTCCTTTGT GGATCAAGTA CGAGAAATTA TTGAAAAGGC TGATGAAATG 
CTCAGTGAGG ATGTCAGTGT GGAACCAGAG GGTGACCAGG GATTGGAGAG TCTGCAGGGA 
AAGGATGACT ATGGCTTTAC AGGTTCTCAA AAATTGGAAG GAGAATTCAA ACAACCAGTT 
CCTGCGTCTT CCATGCCACA GCAAATAGGC ATTCCTACCA GTTCTTTAAC TCAAGTTGTT 
CATTCTGCTG GAAGACGGTT TATAGTGAGT CCTGTGCCAG AAAGTCGATT ACGAGAATCA 
AAAGTTTTCA CTAGTGAAAT ACCAGATACA GTTGCTGCCT CTACTCACAG CGCTGGAATG 
AACTTGTCTC ACTCTGCTTC ATCCCTTAGC CTACAGCAAG CCTTTTCTGA ACTTAGACAT 
GTGCAGATGA CAGAAGGGCC CAGCACAGCA CCTCCCAACT TCAGTCATAC AGGACCAACA 
TTTCCAGTTG TTCCTCCTTC CATGAGTAGC ATTGCTGGAG TCCCAACCAC AGCAGTAGCC 
ACACCTTCAG TATCAGTCCC TGCAACTAGC AGCCCTCTAA ATGACAGTTC CACATCAGTA 
ATTCAATCTG AGATTCCAGT GCCCACTGAA ACAGGGGTTG GTGGAGTTCC CACCTGCACA 
AGTGTAATGC CTTCAAGTGG TCTCCCTGTA CCACCCATAT CTGAATCGCC AATATTTTCC 
ACTATGGTTT CAAGCATCAC AGTACCTACA GTTGTCTCAA TATCAGCAAC ATCCCAGCCA 
GTTCAAGCTC CCACATCTGG GAGCACTGTT TCTAGCATAG GAACTTTGCC TTCTATACCA 
GTTTCAACAG CTTTAGCCTC TGCAGTGGGC AGTGTTGCTA TCCCAGGTGC TAAGCCTCCA 
CCTGTACTAT CACAGCAGGC AGCAGGCAGT ACTACTGGGG CAGCCACATT AACATCCGTT 
CCTGCCATCA CTCCGTTCCC AAGCATAGCT TCACAGCCAT CTCTTCAGCT TAGCAGCAGC 
ACCTCTGCTC CAACTCTAGC TGAAACAGTG GTGGTTAGTG CACACTCACT AGATAAAACA 
TCTCATAGCA GTACAACTGG ATTGGCTTTG TCCCTGTCTG CATCATCTTC CTCCCCTGGA 
GCAGGATTAT CTAGTTCTAT TTCTCAGCCT GGTGGGGTGC ATCCTTTGGT TACCCCATCA 
GCTATGGCTT CTATTCCTGC CCTTCCTCAA ACAGCAGGAC CTACTTCTAC ACCTTTATTG 
CCCCAAGTAC TTAGTATCCC ACCCTTGGTA CAACCTGTTG CCAGTGTGCC TGCTGTGCAG 
CAGACACTAA TTCATAGTCA GCCTCAACCA GCTTTACTTC CCAACCAGCC CCACACTCAC 
TGTCCTGAAA TAGATGCTGA CTTACAGCCC AAAGCTCCTG GAATTGATGA CATAAAGACT 
CTAGAGGAAA AGCTGCGGTC TCTCTTTAGT GAACCGAGCT CATCTGGAGC CCAACATACA 
TCGGTCTCAC TAGAGACTTC ACTGGTAGTA GAGACCACTG TCACGCCAGG TATCCCAACC 
ACTGCTATTG CACCAAGCAA GCTCATGACA TCTACTACAA GTACATGCTT ACCACCAACC 
AGTTTGCAAT TAGGAACATC TGGTTTGTCA GTTATACCAG TGGTCACGCC TGGGCAGGTT 
TCTACCCCAG TCAGCTATGT GTCAGCCCCA GGCAGCACTA CATCAGGAGT GAAAGCTGGA 
ACTGCTCCAT CAAAGCCACC TTTGACTAAA GCTCCAGTGC TGCCAGTGGG TACTGAACTT 
CCAGCTGGTA CTCCACCCAG CGAGCAGCTG CCACCTTTTC CAGGACCTTC ACTAACTCAG 
TCCCAGCAAC CTCTGGAAGA TCTTGATGCT CAGTTGAGAA GAACACTTAG TCCAGAGACT 
ATTGCGGTGA CATCTACAAT TGGTCCTGTG TCCATGGTGG CTCCAACAGC AGTCACAGAA 
GCAGGAGCAC AGCCTCAGAA GGATGTTTCT CAGATCACAG AAGGTCCTGT CCTAGCGACT 
AGTTCGGGAA CTGGTGTTTT TAAGATGGGA AGATTTCAGG TTTCAGTTGC AATGGATGAT 
GTTCAAAAGG AGGGTAAAAA TAAGTTAGAA GATGGAAAGT CTGTTCATTT TGAATCCAGC 
ACTTCAGAGT CCTCAGTGCT ATCAAGTAGT AGTCCAGAAA GTACCCTGGT GAAGACAGAG 
CCTAATGGGA TAACCATCCC TGGCATCTCA TCAGATATGC CAGATAGTGC CCACAAAACT 
CCTGCCTCAG AAGCAAAGTC AGAAACTGGG CAGCCTACCA AGGTTGGACG TTTTCAGGTG 
ACAACTACAG CAAACAAAGT AGGTCGTTTC TCTGTGTCAA GAACTGAAGA CAAGCTCATT 
GAGGCAAAGA AAGAGGGACC AATAGCGTCT CCTCCTTTTA TGGATTTGGA ACAAGGTGTT 
CTTCCAGCTG TGATACCAAA GAAAGAAAAG CCTGAACTGT CAGAGCCTTC ACATCTGAAC 
GGACCATCTT CTGACCTGGA GGCTGCCTTC CTGAGCAGGG ATGTGGATGA TGGTTCAGGT 
AGTCCACACT CCCCCCATCA GCTGAGCTCA AAGAGCCTGC CTATCCAGAA TCTAAGTCAA 
AGCCTTAGTA ATTCATTCAA CTCCTCTTAC ATGAGTAGTG ACAATGAGTC AGATATTGAA 
GATGAAGATT TAAAATTAGA GCTGCGAAGA CTACGAGAAA AACATCTTAA AGAGATTCAA 
GACCTGCAGA GTCGCCAGAA GCATGAAATT GAATCTTTGT ATACCAAACT GGGAAAGGTT 
CCCCCTGCTG TCATTATCCC CCCAGCTGCC CCCCTTTCAG GGAGAAGAAG GCGACCCACT 
AAAAGCAAAG GCAGCAAGTC TAGTCGCAGC AGTTCCTTGG GGAATAAAAG TCCCCAGCTT 
TCAGGTAACC TGTCTGGTCA GAGTGCTGCT TCAGTTTTGC ACGCTCAGCA GACCCTCCAC 
CCTCCTGGCA ACATCCCTGA GACTGGGCCA AATCAGCTGT TACAGCCCCT TAAGCCATCT 
CCCTCCAGTG ACAACCTCTA CTCAGCCTTT ACCAGTGATG GTGCCATTTC AGTCCCAAGC 
CTTTCTGCTC CAGGTCAAGG GACCAGCAGC ACAAACACTG TCGGGGGAAC AGTGAACAGC 
CAAGCTGCCC CAGCTCAGCC TCCTGCCATG ACATCCAGCA GGAAGGGCAC ATTCACAGAT 
GACCTACACA AGTTGGTAGA CAATTGGGCC CGAGATGCCA TGAATCTCTC AGGCAGAAGA 
GGAAGCAAAG GACACATGAA TTATGAGGGC CCTGGAATGG CAAGAAAGTT CTCCGCACCT 
GGTCAGCTGT GCATCTCCAT GACCTCAAAT CTGGGTGGTT CTGCCCCCAT CTCTGCAGCA 
TCAGCTACCT CTCTAGGTCA CTTCACCAAG TCTATGTGCC CCCCACAGCA GTACGGTTTT 
CCAGCTCCCC CCTTTGGCAC TCAGTGGAGT GGGACGGGTG GCCCGGCACC ACAGCCACTT 
GGCCAGTTCC AGCCTGTGGG AACTGCCTCC TTACAGAATT TCAACATCAG CAATTTGCAG 
AAATCCATCA GCAACCCCCC AGGTTCCAAC CTGCGGACCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963816.1
CDS	99..6803
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVGQSQGQPSSSSLTGIPSSLPIQHSQQQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQGFPSRLPPQYPGDSNIAPSSSVASICIPSTVLSPPMPTEALATPGYFPTVVQPFVESNLLVPVGSIGGQVQVSQPAVSLAQQAPTTSSQQAALESTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_014108341.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108341.1

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGGGCTT TCCATCTCGA 
CTGCCACCAC AGTACCCAGG AGATTCAAAT ATTGCTCCTT CTTCCAGCGT GGCTTCTATT 
TGCATCCCTT CTACAGTCCT ATCCCCTCCC ATGCCCACAG AAGCACTGGC TACACCGGGT 
TACTTTCCTA CAGTGGTGCA GCCTTTTGTG GAATCTAACC TTTTGGTTCC TGTGGGCAGT 
ATAGGAGGAC AGGTTCAAGT GTCCCAGCCA GCAGTGAGTT TAGCACAACA AGCCCCCACT 
ACATCCTCCC AGCAAGCAGC TCTGGAGAGC ACTCAGGGAG TCTCTCAAGT TGCTCCTCCA 
GAGCCAGTTC CAGTGGCACA GGCACAGCCT ACCACTTTGG TCTCCTCTAT AGACAGTGCA 
CATTCAGATG TTGCTTCAGG AATGAGTGAT GGCAATGAGA ATGTCCCGTC CTCCAGTGGA 
AGGCATGAAG GGAGAGCTAC AAAACGACAT TACCGAAAAT CTGTAAGAAG TCGCTCTCGT 
CATGAAAAGA CTTCGCGTCC AAAATTAAGA ATTTTGAATG TTTCAAATAA AGGAGACCGA 
GTAGTAGAAT GTCAACTAGA GACTCATAAT CGGAAAATGG TTACATTCAA ATTTGATTTA 
GATGGTGACA ATCCTGAGGA GATAGCAACA ATTATGGTGA ACAATGACTT TATTCTAGCA 
ATGGAGAGAG AGTCCTTTGT GGATCAAGTA CGAGAAATTA TTGAAAAGGC TGATGAAATG 
CTCAGTGAGG ATGTCAGTGT GGAACCAGAG GGTGACCAGG GATTGGAGAG TCTGCAGGGA 
AAGGATGACT ATGGCTTTAC AGGTTCTCAA AAATTGGAAG GAGAATTCAA ACAACCAGTT 
CCTGCGTCTT CCATGCCACA GCAAATAGGC ATTCCTACCA GTTCTTTAAC TCAAGTTGTT 
CATTCTGCTG GAAGACGGTT TATAGTGAGT CCTGTGCCAG AAAGTCGATT ACGAGAATCA 
AAAGTTTTCA CTAGTGAAAT ACCAGATACA GTTGCTGCCT CTACTCACAG CGCTGGAATG 
AACTTGTCTC ACTCTGCTTC ATCCCTTAGC CTACAGCAAG CCTTTTCTGA ACTTAGACAT 
GTGCAGATGA CAGAAGGGCC CAGCACAGCA CCTCCCAACT TCAGTCATAC AGGACCAACA 
TTTCCAGTTG TTCCTCCTTC CATGAGTAGC ATTGCTGGAG TCCCAACCAC AGCAGTAGCC 
ACACCTTCAG TATCAGTCCC TGCAACTAGC AGCCCTCTAA ATGACAGTTC CACATCAGTA 
ATTCAATCTG AGATTCCAGT GCCCACTGAA ACAGGGGTTG GTGGAGTTCC CACCTGCACA 
AGTGTAATGC CTTCAAGTGG TCTCCCTGTA CCACCCATAT CTGAATCGCC AATATTTTCC 
ACTATGGTTT CAAGCATCAC AGTACCTACA GTTGTCTCAA TATCAGCAAC ATCCCAGCCA 
GTTCAAGCTC CCACATCTGG GAGCACTGTT TCTAGCATAG GAACTTTGCC TTCTATACCA 
GTTTCAACAG CTTTAGCCTC TGCAGTGGGC AGTGTTGCTA TCCCAGGTGC TAAGCCTCCA 
CCTGTACTAT CACAGCAGGC AGCAGGCAGT ACTACTGGGG CAGCCACATT AACATCCGTT 
CCTGCCATCA CTCCGTTCCC AAGCATAGCT TCACAGCCAT CTCTTCAGCT TAGCAGCAGC 
ACCTCTGCTC CAACTCTAGC TGAAACAGTG GTGGTTAGTG CACACTCACT AGATAAAACA 
TCTCATAGCA GTACAACTGG ATTGGCTTTG TCCCTGTCTG CATCATCTTC CTCCCCTGGA 
GCAGGATTAT CTAGTTCTAT TTCTCAGCCT GGTGGGGTGC ATCCTTTGGT TACCCCATCA 
GCTATGGCTT CTATTCCTGC CCTTCCTCAA ACAGCAGGAC CTACTTCTAC ACCTTTATTG 
CCCCAAGTAC TTAGTATCCC ACCCTTGGTA CAACCTGTTG CCAGTGTGCC TGCTGTGCAG 
CAGACACTAA TTCATAGTCA GCCTCAACCA GCTTTACTTC CCAACCAGCC CCACACTCAC 
TGTCCTGAAA TAGATGCTGA CTTACAGCCC AAAGCTCCTG GAATTGATGA CATAAAGACT 
CTAGAGGAAA AGCTGCGGTC TCTCTTTAGT GAACCGAGCT CATCTGGAGC CCAACATACA 
TCGGTCTCAC TAGAGACTTC ACTGGTAGTA GAGACCACTG TCACGCCAGG TATCCCAACC 
ACTGCTATTG CACCAAGCAA GCTCATGACA TCTACTACAA GTACATGCTT ACCACCAACC 
AGTTTGCAAT TAGGAACATC TGGTTTGTCA GTTATACCAG TGGTCACGCC TGGGCAGGTT 
TCTACCCCAG TCAGCTATGT GTCAGCCCCA GGCAGCACTA CATCAGGAGT GAAAGCTGGA 
ACTGCTCCAT CAAAGCCACC TTTGACTAAA GCTCCAGTGC TGCCAGTGGG TACTGAACTT 
CCAGCTGGTA CTCCACCCAG CGAGCAGCTG CCACCTTTTC CAGGACCTTC ACTAACTCAG 
TCCCAGCAAC CTCTGGAAGA TCTTGATGCT CAGTTGAGAA GAACACTTAG TCCAGAGACT 
ATTGCGGTGA CATCTACAAT TGGTCCTGTG TCCATGGTGG CTCCAACAGC AGTCACAGAA 
GCAGGAGCAC AGCCTCAGAA GGATGTTTCT CAGATCACAG AAGGTCCTGT CCTAGCGACT 
AGTTCGGGAA CTGGTGTTTT TAAGATGGGA AGATTTCAGG TTTCAGTTGC AATGGATGAT 
GTTCAAAAGG AGGGTAAAAA TAAGTTAGAA GATGGAAAGT CTGTTCATTT TGAATCCAGC 
ACTTCAGAGT CCTCAGTGCT ATCAAGTAGT AGTCCAGAAA GTACCCTGGT GAAGACAGAG 
CCTAATGGGA TAACCATCCC TGGCATCTCA TCAGATATGC CAGATAGTGC CCACAAAACT 
CCTGCCTCAG AAGCAAAGTC AGAAACTGGG CAGCCTACCA AGGTTGGACG TTTTCAGGTG 
ACAACTACAG CAAACAAAGT AGGTCGTTTC TCTGTGTCAA GAACTGAAGA CAAGCTCATT 
GAGGCAAAGA AAGAGGGACC AATAGCGTCT CCTCCTTTTA TGGATTTGGA ACAAGGTGTT 
CTTCCAGCTG TGATACCAAA GAAAGAAAAG CCTGAACTGT CAGAGCCTTC ACATCTGAAC 
GGACCATCTT CTGACCTGGA GGCTGCCTTC CTGAGCAGGG ATGTGGATGA TGGTTCAGGT 
AGTCCACACT CCCCCCATCA GCTGAGCTCA AAGAGCCTGC CTATCCAGAA TCTAAGTCAA 
AGCCTTAGTA ATTCATTCAA CTCCTCTTAC ATGAGTAGTG ACAATGAGTC AGATATTGAA 
GATGAAGATT TAAAATTAGA GCTGCGAAGA CTACGAGAAA AACATCTTAA AGAGATTCAA 
GACCTGCAGA GTCGCCAGAA GCATGAAATT GAATCTTTGT ATACCAAACT GGGAAAGGTT 
CCCCCTGCTG TCATTATCCC CCCAGCTGCC CCCCTTTCAG GGAGAAGAAG GCGACCCACT 
AAAAGCAAAG GCAGCAAGTC TAGTCGCAGC AGTTCCTTGG GGAATAAAAG TCCCCAGCTT 
TCAGGTAACC TGTCTGGTCA GAGTGCTGCT TCAGTTTTGC ACGCTCAGCA GACCCTCCAC 
CCTCCTGGCA ACATCCCTGA GACTGGGCCA AATCAGCTGT TACAGCCCCT TAAGCCATCT 
CCCTCCAGTG ACAACCTCTA CTCAGCCTTT ACCAGTGATG GTGCCATTTC AGTCCCAAGC 
CTTTCTGCTC CAGGTCAAGG GACCAGCAGC ACAAACACTG TCGGGGGAAC AGTGAACAGC 
CAAGCTGCCC CAGCTCAGCC TCCTGCCATG ACATCCAGCA GGAAGGGCAC ATTCACAGAT 
GACCTACACA AGTTGGTAGA CAATTGGGCC CGAGATGCCA TGAATCTCTC AGGCAGAAGA 
GGAAGCAAAG GACACATGAA TTATGAGGGC CCTGGAATGG CAAGAAAGTT CTCCGCACCT 
GGTCAGCTGT GCATCTCCAT GACCTCAAAT CTGGGTGGTT CTGCCCCCAT CTCTGCAGCA 
TCAGCTACCT CTCTAGGTCA CTTCACCAAG TCTATGTGCC CCCCACAGCA GTACGGTTTT 
CCAGCTCCCC CCTTTGGCAC TCAGTGGAGT GGGACGGGTG GCCCGGCACC ACAGCCACTT 
GGCCAGTTCC AGCCTGTGGG AACTGCCTCC TTACAGAATT TCAACATCAG CAATTTGCAG 
AAATCCATCA GCAACCCCCC AGGTTCCAAC CTGCGGACCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_014108342.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963817.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X18, mRNA.

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CloneID	OCe43586
Clone ID Related Accession (Same CDS sequence)	XM_014108342.1
Accession Version	XM_014108342.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase WNK1 isoform X18
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGAGCAC TCAGGGAGTC 
TCTCAAGTTG CTCCTCCAGA GCCAGTTCCA GTGGCACAGG CACAGCCTAC CACTTTGGTC 
TCCTCTATAG ACAGTGCACA TTCAGATGTT GCTTCAGGAA TGAGTGATGG CAATGAGAAT 
GTCCCGTCCT CCAGTGGAAG GCATGAAGGG AGAGCTACAA AACGACATTA CCGAAAATCT 
GTAAGAAGTC GCTCTCGTCA TGAAAAGACT TCGCGTCCAA AATTAAGAAT TTTGAATGTT 
TCAAATAAAG GAGACCGAGT AGTAGAATGT CAACTAGAGA CTCATAATCG GAAAATGGTT 
ACATTCAAAT TTGATTTAGA TGGTGACAAT CCTGAGGAGA TAGCAACAAT TATGGTGAAC 
AATGACTTTA TTCTAGCAAT GGAGAGAGAG TCCTTTGTGG ATCAAGTACG AGAAATTATT 
GAAAAGGCTG ATGAAATGCT CAGTGAGGAT GTCAGTGTGG AACCAGAGGG TGACCAGGGA 
TTGGAGAGTC TGCAGGGAAA GGATGACTAT GGCTTTACAG GTTCTCAAAA ATTGGAAGGA 
GAATTCAAAC AACCAGTTCC TGCGTCTTCC ATGCCACAGC AAATAGGCAT TCCTACCAGT 
TCTTTAACTC AAGTTGTTCA TTCTGCTGGA AGACGGTTTA TAGTGAGTCC TGTGCCAGAA 
AGTCGATTAC GAGAATCAAA AGTTTTCACT AGTGAAATAC CAGATACAGT TGCTGCCTCT 
ACTCACAGCG CTGGAATGAA CTTGTCTCAC TCTGCTTCAT CCCTTAGCCT ACAGCAAGCC 
TTTTCTGAAC TTAGACATGT GCAGATGACA GAAGGGCCCA GCACAGCACC TCCCAACTTC 
AGTCATACAG GACCAACATT TCCAGTTGTT CCTCCTTCCA TGAGTAGCAT TGCTGGAGTC 
CCAACCACAG CAGTAGCCAC ACCTTCAGTA TCAGTCCCTG CAACTAGCAG CCCTCTAAAT 
GACAGTTCCA CATCAGTAAT TCAATCTGAG ATTCCAGTGC CCACTGAAAC AGGGGTTGGT 
GGAGTTCCCA CCTGCACAAG TGTAATGCCT TCAAGTGGTC TCCCTGTACC ACCCATATCT 
GAATCGCCAA TATTTTCCAC TATGGTTTCA AGCATCACAG TACCTACAGT TGTCTCAATA 
TCAGCAACAT CCCAGCCAGT TCAAGCTCCC ACATCTGGGA GCACTGTTTC TAGCATAGGA 
ACTTTGCCTT CTATACCAGT TTCAACAGCT TTAGCCTCTG CAGTGGGCAG TGTTGCTATC 
CCAGGTGCTA AGCCTCCACC TGTACTATCA CAGCAGGCAG CAGGCAGTAC TACTGGGGCA 
GCCACATTAA CATCCGTTCC TGCCATCACT CCGTTCCCAA GCATAGCTTC ACAGCCATCT 
CTTCAGCTTA GCAGCAGCAC CTCTGCTCCA ACTCTAGCTG AAACAGTGGT GGTTAGTGCA 
CACTCACTAG ATAAAACATC TCATAGCAGT ACAACTGGAT TGGCTTTGTC CCTGTCTGCA 
TCATCTTCCT CCCCTGGAGC AGGATTATCT AGTTCTATTT CTCAGCCTGG TGGGGTGCAT 
CCTTTGGTTA CCCCATCAGC TATGGCTTCT ATTCCTGCCC TTCCTCAAAC AGCAGGACCT 
ACTTCTACAC CTTTATTGCC CCAAGTACTT AGTATCCCAC CCTTGGTACA ACCTGTTGCC 
AGTGTGCCTG CTGTGCAGCA GACACTAATT CATAGTCAGC CTCAACCAGC TTTACTTCCC 
AACCAGCCCC ACACTCACTG TCCTGAAATA GATGCTGACT TACAGCCCAA AGCTCCTGGA 
ATTGATGACA TAAAGACTCT AGAGGAAAAG CTGCGGTCTC TCTTTAGTGA ACCGAGCTCA 
TCTGGAGCCC AACATACATC GGTCTCACTA GAGACTTCAC TGGTAGTAGA GACCACTGTC 
ACGCCAGGTA TCCCAACCAC TGCTATTGCA CCAAGCAAGC TCATGACATC TACTACAAGT 
ACATGCTTAC CACCAACCAG TTTGCAATTA GGAACATCTG GTTTGTCAGT TATACCAGTG 
GTCACGCCTG GGCAGGTTTC TACCCCAGTC AGCTATGTGT CAGCCCCAGG CAGCACTACA 
TCAGGAGTGA AAGCTGGAAC TGCTCCATCA AAGCCACCTT TGACTAAAGC TCCAGTGCTG 
CCAGTGGGTA CTGAACTTCC AGCTGGTACT CCACCCAGCG AGCAGCTGCC ACCTTTTCCA 
GGACCTTCAC TAACTCAGTC CCAGCAACCT CTGGAAGATC TTGATGCTCA GTTGAGAAGA 
ACACTTAGTC CAGAGACTAT TGCGGTGACA TCTACAATTG GTCCTGTGTC CATGGTGGCT 
CCAACAGCAG TCACAGAAGC AGGAGCACAG CCTCAGAAGG ATGTTTCTCA GATCACAGAA 
GGTCCTGTCC TAGCGACTAG TTCGGGAACT GGTGTTTTTA AGATGGGAAG ATTTCAGGTT 
TCAGTTGCAA TGGATGATGT TCAAAAGGAG GGTAAAAATA AGTTAGAAGA TGGAAAGTCT 
GTTCATTTTG AATCCAGCAC TTCAGAGTCC TCAGTGCTAT CAAGTAGTAG TCCAGAAAGT 
ACCCTGGTGA AGACAGAGCC TAATGGGATA ACCATCCCTG GCATCTCATC AGATATGCCA 
GATAGTGCCC ACAAAACTCC TGCCTCAGAA GCAAAGTCAG AAACTGGGCA GCCTACCAAG 
GTTGGACGTT TTCAGGTGAC AACTACAGCA AACAAAGTAG GTCGTTTCTC TGTGTCAAGA 
ACTGAAGACA AGCTCATTGA GGCAAAGAAA GAGGGACCAA TAGCGTCTCC TCCTTTTATG 
GATTTGGAAC AAGGTGTTCT TCCAGCTGTG ATACCAAAGA AAGAAAAGCC TGAACTGTCA 
GAGCCTTCAC ATCTGAACGG ACCATCTTCT GACCTGGAGG CTGCCTTCCT GAGCAGGGAT 
GTGGATGATG GTTCAGGTAG TCCACACTCC CCCCATCAGC TGAGCTCAAA GAGCCTGCCT 
ATCCAGAATC TAAGTCAAAG CCTTAGTAAT TCATTCAACT CCTCTTACAT GAGTAGTGAC 
AATGAGTCAG ATATTGAAGA TGAAGATTTA AAATTAGAGC TGCGAAGACT ACGAGAAAAA 
CATCTTAAAG AGATTCAAGA CCTGCAGAGT CGCCAGAAGC ATGAAATTGA ATCTTTGTAT 
ACCAAACTGG GAAAGGTTCC CCCTGCTGTC ATTATCCCCC CAGCTGCCCC CCTTTCAGGG 
AGAAGAAGGC GACCCACTAA AAGCAAAGGC AGCAAGTCTA GTCGCAGCAG TTCCTTGGGG 
AATAAAAGTC CCCAGCTTTC AGGTAACCTG TCTGGTCAGA GTGCTGCTTC AGTTTTGCAC 
GCTCAGCAGA CCCTCCACCC TCCTGGCAAC ATCCCTGAGA CTGGGCCAAA TCAGCTGTTA 
CAGCCCCTTA AGCCATCTCC CTCCAGTGAC AACCTCTACT CAGCCTTTAC CAGTGATGGT 
GCCATTTCAG TCCCAAGCCT TTCTGCTCCA GGTCAAGGCT GTGCGAAGTT TAACTGTGCA 
TCTGAGCAGG TGACATTCAA ACCTGGTGGC AGGAGGACCC GATTTCTGAG TACGCCCTGC 
TTGGCTCTTT GGAAGATGGT GAAAAAAGTC TGTCCTTGCA ACCAGCTCTG TATGTGCCCT 
CCAGCAGAGC CGAAATCAGG GACCAGCAGC ACAAACACTG TCGGGGGAAC AGTGAACAGC 
CAAGCTGCCC CAGCTCAGCC TCCTGCCATG ACATCCAGCA GGAAGGGCAC ATTCACAGAT 
GACCTACACA AGTTGGTAGA CAATTGGGCC CGAGATGCCA TGAATCTCTC AGGCAGAAGA 
GGAAGCAAAG GACACATGAA TTATGAGGGC CCTGGAATGG CAAGAAAGTT CTCCGCACCT 
GGTCAGCTGT GCATCTCCAT GACCTCAAAT CTGGGTGGTT CTGCCCCCAT CTCTGCAGCA 
TCAGCTACCT CTCTAGGTCA CTTCACCAAG TCTATGTGCC CCCCACAGCA GTACGGTTTT 
CCAGCTCCCC CCTTTGGCAC TCAGTGGAGT GGGACGGGTG GCCCGGCACC ACAGCCACTT 
GGCCAGTTCC AGCCTGTGGG AACTGCCTCC TTACAGAATT TCAACATCAG CAATTTGCAG 
AAATCCATCA GCAACCCCCC AGGTTCCAAC CTGCGGACCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963817.1
CDS	99..6683
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVGQSQGQPSSSSLTGIPSSLPIQHSQQQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQSTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGCAKFNCASEQVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCMCPPAEPKSGTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_014108342.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108342.1

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGAGCAC TCAGGGAGTC 
TCTCAAGTTG CTCCTCCAGA GCCAGTTCCA GTGGCACAGG CACAGCCTAC CACTTTGGTC 
TCCTCTATAG ACAGTGCACA TTCAGATGTT GCTTCAGGAA TGAGTGATGG CAATGAGAAT 
GTCCCGTCCT CCAGTGGAAG GCATGAAGGG AGAGCTACAA AACGACATTA CCGAAAATCT 
GTAAGAAGTC GCTCTCGTCA TGAAAAGACT TCGCGTCCAA AATTAAGAAT TTTGAATGTT 
TCAAATAAAG GAGACCGAGT AGTAGAATGT CAACTAGAGA CTCATAATCG GAAAATGGTT 
ACATTCAAAT TTGATTTAGA TGGTGACAAT CCTGAGGAGA TAGCAACAAT TATGGTGAAC 
AATGACTTTA TTCTAGCAAT GGAGAGAGAG TCCTTTGTGG ATCAAGTACG AGAAATTATT 
GAAAAGGCTG ATGAAATGCT CAGTGAGGAT GTCAGTGTGG AACCAGAGGG TGACCAGGGA 
TTGGAGAGTC TGCAGGGAAA GGATGACTAT GGCTTTACAG GTTCTCAAAA ATTGGAAGGA 
GAATTCAAAC AACCAGTTCC TGCGTCTTCC ATGCCACAGC AAATAGGCAT TCCTACCAGT 
TCTTTAACTC AAGTTGTTCA TTCTGCTGGA AGACGGTTTA TAGTGAGTCC TGTGCCAGAA 
AGTCGATTAC GAGAATCAAA AGTTTTCACT AGTGAAATAC CAGATACAGT TGCTGCCTCT 
ACTCACAGCG CTGGAATGAA CTTGTCTCAC TCTGCTTCAT CCCTTAGCCT ACAGCAAGCC 
TTTTCTGAAC TTAGACATGT GCAGATGACA GAAGGGCCCA GCACAGCACC TCCCAACTTC 
AGTCATACAG GACCAACATT TCCAGTTGTT CCTCCTTCCA TGAGTAGCAT TGCTGGAGTC 
CCAACCACAG CAGTAGCCAC ACCTTCAGTA TCAGTCCCTG CAACTAGCAG CCCTCTAAAT 
GACAGTTCCA CATCAGTAAT TCAATCTGAG ATTCCAGTGC CCACTGAAAC AGGGGTTGGT 
GGAGTTCCCA CCTGCACAAG TGTAATGCCT TCAAGTGGTC TCCCTGTACC ACCCATATCT 
GAATCGCCAA TATTTTCCAC TATGGTTTCA AGCATCACAG TACCTACAGT TGTCTCAATA 
TCAGCAACAT CCCAGCCAGT TCAAGCTCCC ACATCTGGGA GCACTGTTTC TAGCATAGGA 
ACTTTGCCTT CTATACCAGT TTCAACAGCT TTAGCCTCTG CAGTGGGCAG TGTTGCTATC 
CCAGGTGCTA AGCCTCCACC TGTACTATCA CAGCAGGCAG CAGGCAGTAC TACTGGGGCA 
GCCACATTAA CATCCGTTCC TGCCATCACT CCGTTCCCAA GCATAGCTTC ACAGCCATCT 
CTTCAGCTTA GCAGCAGCAC CTCTGCTCCA ACTCTAGCTG AAACAGTGGT GGTTAGTGCA 
CACTCACTAG ATAAAACATC TCATAGCAGT ACAACTGGAT TGGCTTTGTC CCTGTCTGCA 
TCATCTTCCT CCCCTGGAGC AGGATTATCT AGTTCTATTT CTCAGCCTGG TGGGGTGCAT 
CCTTTGGTTA CCCCATCAGC TATGGCTTCT ATTCCTGCCC TTCCTCAAAC AGCAGGACCT 
ACTTCTACAC CTTTATTGCC CCAAGTACTT AGTATCCCAC CCTTGGTACA ACCTGTTGCC 
AGTGTGCCTG CTGTGCAGCA GACACTAATT CATAGTCAGC CTCAACCAGC TTTACTTCCC 
AACCAGCCCC ACACTCACTG TCCTGAAATA GATGCTGACT TACAGCCCAA AGCTCCTGGA 
ATTGATGACA TAAAGACTCT AGAGGAAAAG CTGCGGTCTC TCTTTAGTGA ACCGAGCTCA 
TCTGGAGCCC AACATACATC GGTCTCACTA GAGACTTCAC TGGTAGTAGA GACCACTGTC 
ACGCCAGGTA TCCCAACCAC TGCTATTGCA CCAAGCAAGC TCATGACATC TACTACAAGT 
ACATGCTTAC CACCAACCAG TTTGCAATTA GGAACATCTG GTTTGTCAGT TATACCAGTG 
GTCACGCCTG GGCAGGTTTC TACCCCAGTC AGCTATGTGT CAGCCCCAGG CAGCACTACA 
TCAGGAGTGA AAGCTGGAAC TGCTCCATCA AAGCCACCTT TGACTAAAGC TCCAGTGCTG 
CCAGTGGGTA CTGAACTTCC AGCTGGTACT CCACCCAGCG AGCAGCTGCC ACCTTTTCCA 
GGACCTTCAC TAACTCAGTC CCAGCAACCT CTGGAAGATC TTGATGCTCA GTTGAGAAGA 
ACACTTAGTC CAGAGACTAT TGCGGTGACA TCTACAATTG GTCCTGTGTC CATGGTGGCT 
CCAACAGCAG TCACAGAAGC AGGAGCACAG CCTCAGAAGG ATGTTTCTCA GATCACAGAA 
GGTCCTGTCC TAGCGACTAG TTCGGGAACT GGTGTTTTTA AGATGGGAAG ATTTCAGGTT 
TCAGTTGCAA TGGATGATGT TCAAAAGGAG GGTAAAAATA AGTTAGAAGA TGGAAAGTCT 
GTTCATTTTG AATCCAGCAC TTCAGAGTCC TCAGTGCTAT CAAGTAGTAG TCCAGAAAGT 
ACCCTGGTGA AGACAGAGCC TAATGGGATA ACCATCCCTG GCATCTCATC AGATATGCCA 
GATAGTGCCC ACAAAACTCC TGCCTCAGAA GCAAAGTCAG AAACTGGGCA GCCTACCAAG 
GTTGGACGTT TTCAGGTGAC AACTACAGCA AACAAAGTAG GTCGTTTCTC TGTGTCAAGA 
ACTGAAGACA AGCTCATTGA GGCAAAGAAA GAGGGACCAA TAGCGTCTCC TCCTTTTATG 
GATTTGGAAC AAGGTGTTCT TCCAGCTGTG ATACCAAAGA AAGAAAAGCC TGAACTGTCA 
GAGCCTTCAC ATCTGAACGG ACCATCTTCT GACCTGGAGG CTGCCTTCCT GAGCAGGGAT 
GTGGATGATG GTTCAGGTAG TCCACACTCC CCCCATCAGC TGAGCTCAAA GAGCCTGCCT 
ATCCAGAATC TAAGTCAAAG CCTTAGTAAT TCATTCAACT CCTCTTACAT GAGTAGTGAC 
AATGAGTCAG ATATTGAAGA TGAAGATTTA AAATTAGAGC TGCGAAGACT ACGAGAAAAA 
CATCTTAAAG AGATTCAAGA CCTGCAGAGT CGCCAGAAGC ATGAAATTGA ATCTTTGTAT 
ACCAAACTGG GAAAGGTTCC CCCTGCTGTC ATTATCCCCC CAGCTGCCCC CCTTTCAGGG 
AGAAGAAGGC GACCCACTAA AAGCAAAGGC AGCAAGTCTA GTCGCAGCAG TTCCTTGGGG 
AATAAAAGTC CCCAGCTTTC AGGTAACCTG TCTGGTCAGA GTGCTGCTTC AGTTTTGCAC 
GCTCAGCAGA CCCTCCACCC TCCTGGCAAC ATCCCTGAGA CTGGGCCAAA TCAGCTGTTA 
CAGCCCCTTA AGCCATCTCC CTCCAGTGAC AACCTCTACT CAGCCTTTAC CAGTGATGGT 
GCCATTTCAG TCCCAAGCCT TTCTGCTCCA GGTCAAGGCT GTGCGAAGTT TAACTGTGCA 
TCTGAGCAGG TGACATTCAA ACCTGGTGGC AGGAGGACCC GATTTCTGAG TACGCCCTGC 
TTGGCTCTTT GGAAGATGGT GAAAAAAGTC TGTCCTTGCA ACCAGCTCTG TATGTGCCCT 
CCAGCAGAGC CGAAATCAGG GACCAGCAGC ACAAACACTG TCGGGGGAAC AGTGAACAGC 
CAAGCTGCCC CAGCTCAGCC TCCTGCCATG ACATCCAGCA GGAAGGGCAC ATTCACAGAT 
GACCTACACA AGTTGGTAGA CAATTGGGCC CGAGATGCCA TGAATCTCTC AGGCAGAAGA 
GGAAGCAAAG GACACATGAA TTATGAGGGC CCTGGAATGG CAAGAAAGTT CTCCGCACCT 
GGTCAGCTGT GCATCTCCAT GACCTCAAAT CTGGGTGGTT CTGCCCCCAT CTCTGCAGCA 
TCAGCTACCT CTCTAGGTCA CTTCACCAAG TCTATGTGCC CCCCACAGCA GTACGGTTTT 
CCAGCTCCCC CCTTTGGCAC TCAGTGGAGT GGGACGGGTG GCCCGGCACC ACAGCCACTT 
GGCCAGTTCC AGCCTGTGGG AACTGCCTCC TTACAGAATT TCAACATCAG CAATTTGCAG 
AAATCCATCA GCAACCCCCC AGGTTCCAAC CTGCGGACCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_014108343.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963818.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X19, mRNA.

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CloneID	OCe43587
Clone ID Related Accession (Same CDS sequence)	XM_014108343.1 , XM_014108343.2
Accession Version	XM_014108343.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase WNK1 isoform X19
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGAGCAC TCAGGGAGTC 
TCTCAAGTTG CTCCTCCAGA GCCAGTTCCA GTGGCACAGG CACAGCCTAC CACTTTGGTC 
TCCTCTATAG ACAGTGCACA TTCAGATGTT GCTTCAGGAA TGAGTGATGG CAATGAGAAT 
GTCCCGTCCT CCAGTGGAAG GCATGAAGGG AGAGCTACAA AACGACATTA CCGAAAATCT 
GTAAGAAGTC GCTCTCGTCA TGAAAAGACT TCGCGTCCAA AATTAAGAAT TTTGAATGTT 
TCAAATAAAG GAGACCGAGT AGTAGAATGT CAACTAGAGA CTCATAATCG GAAAATGGTT 
ACATTCAAAT TTGATTTAGA TGGTGACAAT CCTGAGGAGA TAGCAACAAT TATGGTGAAC 
AATGACTTTA TTCTAGCAAT GGAGAGAGAG TCCTTTGTGG ATCAAGTACG AGAAATTATT 
GAAAAGGCTG ATGAAATGCT CAGTGAGGAT GTCAGTGTGG AACCAGAGGG TGACCAGGGA 
TTGGAGAGTC TGCAGGGAAA GGATGACTAT GGCTTTACAG GTTCTCAAAA ATTGGAAGGA 
GAATTCAAAC AACCAGTTCC TGCGTCTTCC ATGCCACAGC AAATAGGCAT TCCTACCAGT 
TCTTTAACTC AAGTTGTTCA TTCTGCTGGA AGACGGTTTA TAGTGAGTCC TGTGCCAGAA 
AGTCGATTAC GAGAATCAAA AGTTTTCACT AGTGAAATAC CAGATACAGT TGCTGCCTCT 
ACTCACAGCG CTGGAATGAA CTTGTCTCAC TCTGCTTCAT CCCTTAGCCT ACAGCAAGCC 
TTTTCTGAAC TTAGACATGT GCAGATGACA GAAGGGCCCA GCACAGCACC TCCCAACTTC 
AGTCATACAG GACCAACATT TCCAGTTGTT CCTCCTTCCA TGAGTAGCAT TGCTGGAGTC 
CCAACCACAG CAGTAGCCAC ACCTTCAGTA TCAGTCCCTG CAACTAGCAG CCCTCTAAAT 
GACAGTTCCA CATCAGTAAT TCAATCTGAG ATTCCAGTGC CCACTGAAAC AGGGGTTGGT 
GGAGTTCCCA CCTGCACAAG TGTAATGCCT TCAAGTGGTC TCCCTGTACC ACCCATATCT 
GAATCGCCAA TATTTTCCAC TATGGTTTCA AGCATCACAG TACCTACAGT TGTCTCAATA 
TCAGCAACAT CCCAGCCAGT TCAAGCTCCC ACATCTGGGA GCACTGTTTC TAGCATAGGA 
ACTTTGCCTT CTATACCAGT TTCAACAGCT TTAGCCTCTG CAGTGGGCAG TGTTGCTATC 
CCAGGTGCTA AGCCTCCACC TGTACTATCA CAGCAGGCAG CAGGCAGTAC TACTGGGGCA 
GCCACATTAA CATCCGTTCC TGCCATCACT CCGTTCCCAA GCATAGCTTC ACAGCCATCT 
CTTCAGCTTA GCAGCAGCAC CTCTGCTCCA ACTCTAGCTG AAACAGTGGT GGTTAGTGCA 
CACTCACTAG ATAAAACATC TCATAGCAGT ACAACTGGAT TGGCTTTGTC CCTGTCTGCA 
TCATCTTCCT CCCCTGGAGC AGGATTATCT AGTTCTATTT CTCAGCCTGG TGGGGTGCAT 
CCTTTGGTTA CCCCATCAGC TATGGCTTCT ATTCCTGCCC TTCCTCAAAC AGCAGGACCT 
ACTTCTACAC CTTTATTGCC CCAAGTACTT AGTATCCCAC CCTTGGTACA ACCTGTTGCC 
AGTGTGCCTG CTGTGCAGCA GACACTAATT CATAGTCAGC CTCAACCAGC TTTACTTCCC 
AACCAGCCCC ACACTCACTG TCCTGAAATA GATGCTGACT TACAGCCCAA AGCTCCTGGA 
ATTGATGACA TAAAGACTCT AGAGGAAAAG CTGCGGTCTC TCTTTAGTGA ACCGAGCTCA 
TCTGGAGCCC AACATACATC GGTCTCACTA GAGACTTCAC TGGTAGTAGA GACCACTGTC 
ACGCCAGGTA TCCCAACCAC TGCTATTGCA CCAAGCAAGC TCATGACATC TACTACAAGT 
ACATGCTTAC CACCAACCAG TTTGCAATTA GGAACATCTG GTTTGTCAGT TATACCAGTG 
GTCACGCCTG GGCAGGTTTC TACCCCAGTC AGCTATGTGT CAGCCCCAGG CAGCACTACA 
TCAGGAGTGA AAGCTGGAAC TGCTCCATCA AAGCCACCTT TGACTAAAGC TCCAGTGCTG 
CCAGTGGGTA CTGAACTTCC AGCTGGTACT CCACCCAGCG AGCAGCTGCC ACCTTTTCCA 
GGACCTTCAC TAACTCAGTC CCAGCAACCT CTGGAAGATC TTGATGCTCA GTTGAGAAGA 
ACACTTAGTC CAGAGACTAT TGCGGTGACA TCTACAATTG GTCCTGTGTC CATGGTGGCT 
CCAACAGCAG TCACAGAAGC AGGAGCACAG CCTCAGAAGG ATGTTTCTCA GATCACAGAA 
GGTCCTGTCC TAGCGACTAG TTCGGGAACT GGTGTTTTTA AGATGGGAAG ATTTCAGGTT 
TCAGTTGCAA TGGATGATGT TCAAAAGGAG GGTAAAAATA AGTTAGAAGA TGGAAAGTCT 
GTTCATTTTG AATCCAGCAC TTCAGAGTCC TCAGTGCTAT CAAGTAGTAG TCCAGAAAGT 
ACCCTGGTGA AGACAGAGCC TAATGGGATA ACCATCCCTG GCATCTCATC AGATATGCCA 
GATAGTGCCC ACAAAACTCC TGCCTCAGAA GCAAAGTCAG AAACTGGGCA GCCTACCAAG 
GTTGGACGTT TTCAGGTGAC AACTACAGCA AACAAAGTAG GTCGTTTCTC TGTGTCAAGA 
ACTGAAGACA AGCTCATTGA GGCAAAGAAA GAGGGACCAA TAGCGTCTCC TCCTTTTATG 
GATTTGGAAC AAGGTGTTCT TCCAGCTGTG ATACCAAAGA AAGAAAAGCC TGAACTGTCA 
GAGCCTTCAC ATCTGAACGG ACCATCTTCT GACCTGGAGG CTGCCTTCCT GAGCAGGGAT 
GTGGATGATG GTTCAGGTAG TCCACACTCC CCCCATCAGC TGAGCTCAAA GAGCCTGCCT 
ATCCAGAATC TAAGTCAAAG CCTTAGTAAT TCATTCAACT CCTCTTACAT GAGTAGTGAC 
AATGAGTCAG ATATTGAAGA TGAAGATTTA AAATTAGAGC TGCGAAGACT ACGAGAAAAA 
CATCTTAAAG AGATTCAAGA CCTGCAGAGT CGCCAGAAGC ATGAAATTGA ATCTTTGTAT 
ACCAAACTGG GAAAGGTTCC CCCTGCTGTC ATTATCCCCC CAGCTGCCCC CCTTTCAGGG 
AGAAGAAGGC GACCCACTAA AAGCAAAGGC AGCAAGTCTA GTCGCAGCAG TTCCTTGGGG 
AATAAAAGTC CCCAGCTTTC AGGTAACCTG TCTGGTCAGA GTGCTGCTTC AGTTTTGCAC 
GCTCAGCAGA CCCTCCACCC TCCTGGCAAC ATCCCTGAGA CTGGGCCAAA TCAGCTGTTA 
CAGCCCCTTA AGCCATCTCC CTCCAGTGAC AACCTCTACT CAGCCTTTAC CAGTGATGGT 
GCCATTTCAG TCCCAAGCCT TTCTGCTCCA GGTCAAGGGA CCAGCAGCAC AAACACTGTC 
GGGGGAACAG TGAACAGCCA AGCTGCCCCA GCTCAGCCTC CTGCCATGAC ATCCAGCAGG 
AAGGGCACAT TCACAGATGA CCTACACAAG TTGGTAGACA ATTGGGCCCG AGATGCCATG 
AATCTCTCAG GCAGAAGAGG AAGCAAAGGA CACATGAATT ATGAGGGCCC TGGAATGGCA 
AGAAAGTTCT CCGCACCTGG TCAGCTGTGC ATCTCCATGA CCTCAAATCT GGGTGGTTCT 
GCCCCCATCT CTGCAGCATC AGCTACCTCT CTAGGTCACT TCACCAAGTC TATGTGCCCC 
CCACAGCAGT ACGGTTTTCC AGCTCCCCCC TTTGGCACTC AGTGGAGTGG GACGGGTGGC 
CCGGCACCAC AGCCACTTGG CCAGTTCCAG CCTGTGGGAA CTGCCTCCTT ACAGAATTTC 
AACATCAGCA ATTTGCAGAA ATCCATCAGC AACCCCCCAG GTTCCAACCT GCGGACCACT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963818.1
CDS	99..6521
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVGQSQGQPSSSSLTGIPSSLPIQHSQQQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQSTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_014108343.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108343.1

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGAGCAC TCAGGGAGTC 
TCTCAAGTTG CTCCTCCAGA GCCAGTTCCA GTGGCACAGG CACAGCCTAC CACTTTGGTC 
TCCTCTATAG ACAGTGCACA TTCAGATGTT GCTTCAGGAA TGAGTGATGG CAATGAGAAT 
GTCCCGTCCT CCAGTGGAAG GCATGAAGGG AGAGCTACAA AACGACATTA CCGAAAATCT 
GTAAGAAGTC GCTCTCGTCA TGAAAAGACT TCGCGTCCAA AATTAAGAAT TTTGAATGTT 
TCAAATAAAG GAGACCGAGT AGTAGAATGT CAACTAGAGA CTCATAATCG GAAAATGGTT 
ACATTCAAAT TTGATTTAGA TGGTGACAAT CCTGAGGAGA TAGCAACAAT TATGGTGAAC 
AATGACTTTA TTCTAGCAAT GGAGAGAGAG TCCTTTGTGG ATCAAGTACG AGAAATTATT 
GAAAAGGCTG ATGAAATGCT CAGTGAGGAT GTCAGTGTGG AACCAGAGGG TGACCAGGGA 
TTGGAGAGTC TGCAGGGAAA GGATGACTAT GGCTTTACAG GTTCTCAAAA ATTGGAAGGA 
GAATTCAAAC AACCAGTTCC TGCGTCTTCC ATGCCACAGC AAATAGGCAT TCCTACCAGT 
TCTTTAACTC AAGTTGTTCA TTCTGCTGGA AGACGGTTTA TAGTGAGTCC TGTGCCAGAA 
AGTCGATTAC GAGAATCAAA AGTTTTCACT AGTGAAATAC CAGATACAGT TGCTGCCTCT 
ACTCACAGCG CTGGAATGAA CTTGTCTCAC TCTGCTTCAT CCCTTAGCCT ACAGCAAGCC 
TTTTCTGAAC TTAGACATGT GCAGATGACA GAAGGGCCCA GCACAGCACC TCCCAACTTC 
AGTCATACAG GACCAACATT TCCAGTTGTT CCTCCTTCCA TGAGTAGCAT TGCTGGAGTC 
CCAACCACAG CAGTAGCCAC ACCTTCAGTA TCAGTCCCTG CAACTAGCAG CCCTCTAAAT 
GACAGTTCCA CATCAGTAAT TCAATCTGAG ATTCCAGTGC CCACTGAAAC AGGGGTTGGT 
GGAGTTCCCA CCTGCACAAG TGTAATGCCT TCAAGTGGTC TCCCTGTACC ACCCATATCT 
GAATCGCCAA TATTTTCCAC TATGGTTTCA AGCATCACAG TACCTACAGT TGTCTCAATA 
TCAGCAACAT CCCAGCCAGT TCAAGCTCCC ACATCTGGGA GCACTGTTTC TAGCATAGGA 
ACTTTGCCTT CTATACCAGT TTCAACAGCT TTAGCCTCTG CAGTGGGCAG TGTTGCTATC 
CCAGGTGCTA AGCCTCCACC TGTACTATCA CAGCAGGCAG CAGGCAGTAC TACTGGGGCA 
GCCACATTAA CATCCGTTCC TGCCATCACT CCGTTCCCAA GCATAGCTTC ACAGCCATCT 
CTTCAGCTTA GCAGCAGCAC CTCTGCTCCA ACTCTAGCTG AAACAGTGGT GGTTAGTGCA 
CACTCACTAG ATAAAACATC TCATAGCAGT ACAACTGGAT TGGCTTTGTC CCTGTCTGCA 
TCATCTTCCT CCCCTGGAGC AGGATTATCT AGTTCTATTT CTCAGCCTGG TGGGGTGCAT 
CCTTTGGTTA CCCCATCAGC TATGGCTTCT ATTCCTGCCC TTCCTCAAAC AGCAGGACCT 
ACTTCTACAC CTTTATTGCC CCAAGTACTT AGTATCCCAC CCTTGGTACA ACCTGTTGCC 
AGTGTGCCTG CTGTGCAGCA GACACTAATT CATAGTCAGC CTCAACCAGC TTTACTTCCC 
AACCAGCCCC ACACTCACTG TCCTGAAATA GATGCTGACT TACAGCCCAA AGCTCCTGGA 
ATTGATGACA TAAAGACTCT AGAGGAAAAG CTGCGGTCTC TCTTTAGTGA ACCGAGCTCA 
TCTGGAGCCC AACATACATC GGTCTCACTA GAGACTTCAC TGGTAGTAGA GACCACTGTC 
ACGCCAGGTA TCCCAACCAC TGCTATTGCA CCAAGCAAGC TCATGACATC TACTACAAGT 
ACATGCTTAC CACCAACCAG TTTGCAATTA GGAACATCTG GTTTGTCAGT TATACCAGTG 
GTCACGCCTG GGCAGGTTTC TACCCCAGTC AGCTATGTGT CAGCCCCAGG CAGCACTACA 
TCAGGAGTGA AAGCTGGAAC TGCTCCATCA AAGCCACCTT TGACTAAAGC TCCAGTGCTG 
CCAGTGGGTA CTGAACTTCC AGCTGGTACT CCACCCAGCG AGCAGCTGCC ACCTTTTCCA 
GGACCTTCAC TAACTCAGTC CCAGCAACCT CTGGAAGATC TTGATGCTCA GTTGAGAAGA 
ACACTTAGTC CAGAGACTAT TGCGGTGACA TCTACAATTG GTCCTGTGTC CATGGTGGCT 
CCAACAGCAG TCACAGAAGC AGGAGCACAG CCTCAGAAGG ATGTTTCTCA GATCACAGAA 
GGTCCTGTCC TAGCGACTAG TTCGGGAACT GGTGTTTTTA AGATGGGAAG ATTTCAGGTT 
TCAGTTGCAA TGGATGATGT TCAAAAGGAG GGTAAAAATA AGTTAGAAGA TGGAAAGTCT 
GTTCATTTTG AATCCAGCAC TTCAGAGTCC TCAGTGCTAT CAAGTAGTAG TCCAGAAAGT 
ACCCTGGTGA AGACAGAGCC TAATGGGATA ACCATCCCTG GCATCTCATC AGATATGCCA 
GATAGTGCCC ACAAAACTCC TGCCTCAGAA GCAAAGTCAG AAACTGGGCA GCCTACCAAG 
GTTGGACGTT TTCAGGTGAC AACTACAGCA AACAAAGTAG GTCGTTTCTC TGTGTCAAGA 
ACTGAAGACA AGCTCATTGA GGCAAAGAAA GAGGGACCAA TAGCGTCTCC TCCTTTTATG 
GATTTGGAAC AAGGTGTTCT TCCAGCTGTG ATACCAAAGA AAGAAAAGCC TGAACTGTCA 
GAGCCTTCAC ATCTGAACGG ACCATCTTCT GACCTGGAGG CTGCCTTCCT GAGCAGGGAT 
GTGGATGATG GTTCAGGTAG TCCACACTCC CCCCATCAGC TGAGCTCAAA GAGCCTGCCT 
ATCCAGAATC TAAGTCAAAG CCTTAGTAAT TCATTCAACT CCTCTTACAT GAGTAGTGAC 
AATGAGTCAG ATATTGAAGA TGAAGATTTA AAATTAGAGC TGCGAAGACT ACGAGAAAAA 
CATCTTAAAG AGATTCAAGA CCTGCAGAGT CGCCAGAAGC ATGAAATTGA ATCTTTGTAT 
ACCAAACTGG GAAAGGTTCC CCCTGCTGTC ATTATCCCCC CAGCTGCCCC CCTTTCAGGG 
AGAAGAAGGC GACCCACTAA AAGCAAAGGC AGCAAGTCTA GTCGCAGCAG TTCCTTGGGG 
AATAAAAGTC CCCAGCTTTC AGGTAACCTG TCTGGTCAGA GTGCTGCTTC AGTTTTGCAC 
GCTCAGCAGA CCCTCCACCC TCCTGGCAAC ATCCCTGAGA CTGGGCCAAA TCAGCTGTTA 
CAGCCCCTTA AGCCATCTCC CTCCAGTGAC AACCTCTACT CAGCCTTTAC CAGTGATGGT 
GCCATTTCAG TCCCAAGCCT TTCTGCTCCA GGTCAAGGGA CCAGCAGCAC AAACACTGTC 
GGGGGAACAG TGAACAGCCA AGCTGCCCCA GCTCAGCCTC CTGCCATGAC ATCCAGCAGG 
AAGGGCACAT TCACAGATGA CCTACACAAG TTGGTAGACA ATTGGGCCCG AGATGCCATG 
AATCTCTCAG GCAGAAGAGG AAGCAAAGGA CACATGAATT ATGAGGGCCC TGGAATGGCA 
AGAAAGTTCT CCGCACCTGG TCAGCTGTGC ATCTCCATGA CCTCAAATCT GGGTGGTTCT 
GCCCCCATCT CTGCAGCATC AGCTACCTCT CTAGGTCACT TCACCAAGTC TATGTGCCCC 
CCACAGCAGT ACGGTTTTCC AGCTCCCCCC TTTGGCACTC AGTGGAGTGG GACGGGTGGC 
CCGGCACCAC AGCCACTTGG CCAGTTCCAG CCTGTGGGAA CTGCCTCCTT ACAGAATTTC 
AACATCAGCA ATTTGCAGAA ATCCATCAGC AACCCCCCAG GTTCCAACCT GCGGACCACT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_014108343.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963818.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X18, mRNA.

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CloneID	OCe43587
Clone ID Related Accession (Same CDS sequence)	XM_014108343.1 , XM_014108343.2
Accession Version	XM_014108343.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase WNK1 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014108343.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGAGCAC TCAGGGAGTC 
TCTCAAGTTG CTCCTCCAGA GCCAGTTCCA GTGGCACAGG CACAGCCTAC CACTTTGGTC 
TCCTCTATAG ACAGTGCACA TTCAGATGTT GCTTCAGGAA TGAGTGATGG CAATGAGAAT 
GTCCCGTCCT CCAGTGGAAG GCATGAAGGG AGAGCTACAA AACGACATTA CCGAAAATCT 
GTAAGAAGTC GCTCTCGTCA TGAAAAGACT TCGCGTCCAA AATTAAGAAT TTTGAATGTT 
TCAAATAAAG GAGACCGAGT AGTAGAATGT CAACTAGAGA CTCATAATCG GAAAATGGTT 
ACATTCAAAT TTGATTTAGA TGGTGACAAT CCTGAGGAGA TAGCAACAAT TATGGTGAAC 
AATGACTTTA TTCTAGCAAT GGAGAGAGAG TCCTTTGTGG ATCAAGTACG AGAAATTATT 
GAAAAGGCTG ATGAAATGCT CAGTGAGGAT GTCAGTGTGG AACCAGAGGG TGACCAGGGA 
TTGGAGAGTC TGCAGGGAAA GGATGACTAT GGCTTTACAG GTTCTCAAAA ATTGGAAGGA 
GAATTCAAAC AACCAGTTCC TGCGTCTTCC ATGCCACAGC AAATAGGCAT TCCTACCAGT 
TCTTTAACTC AAGTTGTTCA TTCTGCTGGA AGACGGTTTA TAGTGAGTCC TGTGCCAGAA 
AGTCGATTAC GAGAATCAAA AGTTTTCACT AGTGAAATAC CAGATACAGT TGCTGCCTCT 
ACTCACAGCG CTGGAATGAA CTTGTCTCAC TCTGCTTCAT CCCTTAGCCT ACAGCAAGCC 
TTTTCTGAAC TTAGACATGT GCAGATGACA GAAGGGCCCA GCACAGCACC TCCCAACTTC 
AGTCATACAG GACCAACATT TCCAGTTGTT CCTCCTTCCA TGAGTAGCAT TGCTGGAGTC 
CCAACCACAG CAGTAGCCAC ACCTTCAGTA TCAGTCCCTG CAACTAGCAG CCCTCTAAAT 
GACAGTTCCA CATCAGTAAT TCAATCTGAG ATTCCAGTGC CCACTGAAAC AGGGGTTGGT 
GGAGTTCCCA CCTGCACAAG TGTAATGCCT TCAAGTGGTC TCCCTGTACC ACCCATATCT 
GAATCGCCAA TATTTTCCAC TATGGTTTCA AGCATCACAG TACCTACAGT TGTCTCAATA 
TCAGCAACAT CCCAGCCAGT TCAAGCTCCC ACATCTGGGA GCACTGTTTC TAGCATAGGA 
ACTTTGCCTT CTATACCAGT TTCAACAGCT TTAGCCTCTG CAGTGGGCAG TGTTGCTATC 
CCAGGTGCTA AGCCTCCACC TGTACTATCA CAGCAGGCAG CAGGCAGTAC TACTGGGGCA 
GCCACATTAA CATCCGTTCC TGCCATCACT CCGTTCCCAA GCATAGCTTC ACAGCCATCT 
CTTCAGCTTA GCAGCAGCAC CTCTGCTCCA ACTCTAGCTG AAACAGTGGT GGTTAGTGCA 
CACTCACTAG ATAAAACATC TCATAGCAGT ACAACTGGAT TGGCTTTGTC CCTGTCTGCA 
TCATCTTCCT CCCCTGGAGC AGGATTATCT AGTTCTATTT CTCAGCCTGG TGGGGTGCAT 
CCTTTGGTTA CCCCATCAGC TATGGCTTCT ATTCCTGCCC TTCCTCAAAC AGCAGGACCT 
ACTTCTACAC CTTTATTGCC CCAAGTACTT AGTATCCCAC CCTTGGTACA ACCTGTTGCC 
AGTGTGCCTG CTGTGCAGCA GACACTAATT CATAGTCAGC CTCAACCAGC TTTACTTCCC 
AACCAGCCCC ACACTCACTG TCCTGAAATA GATGCTGACT TACAGCCCAA AGCTCCTGGA 
ATTGATGACA TAAAGACTCT AGAGGAAAAG CTGCGGTCTC TCTTTAGTGA ACCGAGCTCA 
TCTGGAGCCC AACATACATC GGTCTCACTA GAGACTTCAC TGGTAGTAGA GACCACTGTC 
ACGCCAGGTA TCCCAACCAC TGCTATTGCA CCAAGCAAGC TCATGACATC TACTACAAGT 
ACATGCTTAC CACCAACCAG TTTGCAATTA GGAACATCTG GTTTGTCAGT TATACCAGTG 
GTCACGCCTG GGCAGGTTTC TACCCCAGTC AGCTATGTGT CAGCCCCAGG CAGCACTACA 
TCAGGAGTGA AAGCTGGAAC TGCTCCATCA AAGCCACCTT TGACTAAAGC TCCAGTGCTG 
CCAGTGGGTA CTGAACTTCC AGCTGGTACT CCACCCAGCG AGCAGCTGCC ACCTTTTCCA 
GGACCTTCAC TAACTCAGTC CCAGCAACCT CTGGAAGATC TTGATGCTCA GTTGAGAAGA 
ACACTTAGTC CAGAGACTAT TGCGGTGACA TCTACAATTG GTCCTGTGTC CATGGTGGCT 
CCAACAGCAG TCACAGAAGC AGGAGCACAG CCTCAGAAGG ATGTTTCTCA GATCACAGAA 
GGTCCTGTCC TAGCGACTAG TTCGGGAACT GGTGTTTTTA AGATGGGAAG ATTTCAGGTT 
TCAGTTGCAA TGGATGATGT TCAAAAGGAG GGTAAAAATA AGTTAGAAGA TGGAAAGTCT 
GTTCATTTTG AATCCAGCAC TTCAGAGTCC TCAGTGCTAT CAAGTAGTAG TCCAGAAAGT 
ACCCTGGTGA AGACAGAGCC TAATGGGATA ACCATCCCTG GCATCTCATC AGATATGCCA 
GATAGTGCCC ACAAAACTCC TGCCTCAGAA GCAAAGTCAG AAACTGGGCA GCCTACCAAG 
GTTGGACGTT TTCAGGTGAC AACTACAGCA AACAAAGTAG GTCGTTTCTC TGTGTCAAGA 
ACTGAAGACA AGCTCATTGA GGCAAAGAAA GAGGGACCAA TAGCGTCTCC TCCTTTTATG 
GATTTGGAAC AAGGTGTTCT TCCAGCTGTG ATACCAAAGA AAGAAAAGCC TGAACTGTCA 
GAGCCTTCAC ATCTGAACGG ACCATCTTCT GACCTGGAGG CTGCCTTCCT GAGCAGGGAT 
GTGGATGATG GTTCAGGTAG TCCACACTCC CCCCATCAGC TGAGCTCAAA GAGCCTGCCT 
ATCCAGAATC TAAGTCAAAG CCTTAGTAAT TCATTCAACT CCTCTTACAT GAGTAGTGAC 
AATGAGTCAG ATATTGAAGA TGAAGATTTA AAATTAGAGC TGCGAAGACT ACGAGAAAAA 
CATCTTAAAG AGATTCAAGA CCTGCAGAGT CGCCAGAAGC ATGAAATTGA ATCTTTGTAT 
ACCAAACTGG GAAAGGTTCC CCCTGCTGTC ATTATCCCCC CAGCTGCCCC CCTTTCAGGG 
AGAAGAAGGC GACCCACTAA AAGCAAAGGC AGCAAGTCTA GTCGCAGCAG TTCCTTGGGG 
AATAAAAGTC CCCAGCTTTC AGGTAACCTG TCTGGTCAGA GTGCTGCTTC AGTTTTGCAC 
GCTCAGCAGA CCCTCCACCC TCCTGGCAAC ATCCCTGAGA CTGGGCCAAA TCAGCTGTTA 
CAGCCCCTTA AGCCATCTCC CTCCAGTGAC AACCTCTACT CAGCCTTTAC CAGTGATGGT 
GCCATTTCAG TCCCAAGCCT TTCTGCTCCA GGTCAAGGGA CCAGCAGCAC AAACACTGTC 
GGGGGAACAG TGAACAGCCA AGCTGCCCCA GCTCAGCCTC CTGCCATGAC ATCCAGCAGG 
AAGGGCACAT TCACAGATGA CCTACACAAG TTGGTAGACA ATTGGGCCCG AGATGCCATG 
AATCTCTCAG GCAGAAGAGG AAGCAAAGGA CACATGAATT ATGAGGGCCC TGGAATGGCA 
AGAAAGTTCT CCGCACCTGG TCAGCTGTGC ATCTCCATGA CCTCAAATCT GGGTGGTTCT 
GCCCCCATCT CTGCAGCATC AGCTACCTCT CTAGGTCACT TCACCAAGTC TATGTGCCCC 
CCACAGCAGT ACGGTTTTCC AGCTCCCCCC TTTGGCACTC AGTGGAGTGG GACGGGTGGC 
CCGGCACCAC AGCCACTTGG CCAGTTCCAG CCTGTGGGAA CTGCCTCCTT ACAGAATTTC 
AACATCAGCA ATTTGCAGAA ATCCATCAGC AACCCCCCAG GTTCCAACCT GCGGACCACT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963818.1
CDS	994..7416
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVGQSQGQPSSSSLTGIPSSLPIQHSQQQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQSTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_014108343.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108343.2

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGCA GGGAGTACAG CAGACAGCCC CTCCACAACA GACAGCGCAG 
TATTCGCTTC CACAGACATC AGCCTCCAGT GAGGCCACTA TTGCACAACC AGTCAGTCAG 
TCTCAGGCTC CACAGGTCTT GCCTCAAATA TCAGCTGGAA AACAGAGCAC TCAGGGAGTC 
TCTCAAGTTG CTCCTCCAGA GCCAGTTCCA GTGGCACAGG CACAGCCTAC CACTTTGGTC 
TCCTCTATAG ACAGTGCACA TTCAGATGTT GCTTCAGGAA TGAGTGATGG CAATGAGAAT 
GTCCCGTCCT CCAGTGGAAG GCATGAAGGG AGAGCTACAA AACGACATTA CCGAAAATCT 
GTAAGAAGTC GCTCTCGTCA TGAAAAGACT TCGCGTCCAA AATTAAGAAT TTTGAATGTT 
TCAAATAAAG GAGACCGAGT AGTAGAATGT CAACTAGAGA CTCATAATCG GAAAATGGTT 
ACATTCAAAT TTGATTTAGA TGGTGACAAT CCTGAGGAGA TAGCAACAAT TATGGTGAAC 
AATGACTTTA TTCTAGCAAT GGAGAGAGAG TCCTTTGTGG ATCAAGTACG AGAAATTATT 
GAAAAGGCTG ATGAAATGCT CAGTGAGGAT GTCAGTGTGG AACCAGAGGG TGACCAGGGA 
TTGGAGAGTC TGCAGGGAAA GGATGACTAT GGCTTTACAG GTTCTCAAAA ATTGGAAGGA 
GAATTCAAAC AACCAGTTCC TGCGTCTTCC ATGCCACAGC AAATAGGCAT TCCTACCAGT 
TCTTTAACTC AAGTTGTTCA TTCTGCTGGA AGACGGTTTA TAGTGAGTCC TGTGCCAGAA 
AGTCGATTAC GAGAATCAAA AGTTTTCACT AGTGAAATAC CAGATACAGT TGCTGCCTCT 
ACTCACAGCG CTGGAATGAA CTTGTCTCAC TCTGCTTCAT CCCTTAGCCT ACAGCAAGCC 
TTTTCTGAAC TTAGACATGT GCAGATGACA GAAGGGCCCA GCACAGCACC TCCCAACTTC 
AGTCATACAG GACCAACATT TCCAGTTGTT CCTCCTTCCA TGAGTAGCAT TGCTGGAGTC 
CCAACCACAG CAGTAGCCAC ACCTTCAGTA TCAGTCCCTG CAACTAGCAG CCCTCTAAAT 
GACAGTTCCA CATCAGTAAT TCAATCTGAG ATTCCAGTGC CCACTGAAAC AGGGGTTGGT 
GGAGTTCCCA CCTGCACAAG TGTAATGCCT TCAAGTGGTC TCCCTGTACC ACCCATATCT 
GAATCGCCAA TATTTTCCAC TATGGTTTCA AGCATCACAG TACCTACAGT TGTCTCAATA 
TCAGCAACAT CCCAGCCAGT TCAAGCTCCC ACATCTGGGA GCACTGTTTC TAGCATAGGA 
ACTTTGCCTT CTATACCAGT TTCAACAGCT TTAGCCTCTG CAGTGGGCAG TGTTGCTATC 
CCAGGTGCTA AGCCTCCACC TGTACTATCA CAGCAGGCAG CAGGCAGTAC TACTGGGGCA 
GCCACATTAA CATCCGTTCC TGCCATCACT CCGTTCCCAA GCATAGCTTC ACAGCCATCT 
CTTCAGCTTA GCAGCAGCAC CTCTGCTCCA ACTCTAGCTG AAACAGTGGT GGTTAGTGCA 
CACTCACTAG ATAAAACATC TCATAGCAGT ACAACTGGAT TGGCTTTGTC CCTGTCTGCA 
TCATCTTCCT CCCCTGGAGC AGGATTATCT AGTTCTATTT CTCAGCCTGG TGGGGTGCAT 
CCTTTGGTTA CCCCATCAGC TATGGCTTCT ATTCCTGCCC TTCCTCAAAC AGCAGGACCT 
ACTTCTACAC CTTTATTGCC CCAAGTACTT AGTATCCCAC CCTTGGTACA ACCTGTTGCC 
AGTGTGCCTG CTGTGCAGCA GACACTAATT CATAGTCAGC CTCAACCAGC TTTACTTCCC 
AACCAGCCCC ACACTCACTG TCCTGAAATA GATGCTGACT TACAGCCCAA AGCTCCTGGA 
ATTGATGACA TAAAGACTCT AGAGGAAAAG CTGCGGTCTC TCTTTAGTGA ACCGAGCTCA 
TCTGGAGCCC AACATACATC GGTCTCACTA GAGACTTCAC TGGTAGTAGA GACCACTGTC 
ACGCCAGGTA TCCCAACCAC TGCTATTGCA CCAAGCAAGC TCATGACATC TACTACAAGT 
ACATGCTTAC CACCAACCAG TTTGCAATTA GGAACATCTG GTTTGTCAGT TATACCAGTG 
GTCACGCCTG GGCAGGTTTC TACCCCAGTC AGCTATGTGT CAGCCCCAGG CAGCACTACA 
TCAGGAGTGA AAGCTGGAAC TGCTCCATCA AAGCCACCTT TGACTAAAGC TCCAGTGCTG 
CCAGTGGGTA CTGAACTTCC AGCTGGTACT CCACCCAGCG AGCAGCTGCC ACCTTTTCCA 
GGACCTTCAC TAACTCAGTC CCAGCAACCT CTGGAAGATC TTGATGCTCA GTTGAGAAGA 
ACACTTAGTC CAGAGACTAT TGCGGTGACA TCTACAATTG GTCCTGTGTC CATGGTGGCT 
CCAACAGCAG TCACAGAAGC AGGAGCACAG CCTCAGAAGG ATGTTTCTCA GATCACAGAA 
GGTCCTGTCC TAGCGACTAG TTCGGGAACT GGTGTTTTTA AGATGGGAAG ATTTCAGGTT 
TCAGTTGCAA TGGATGATGT TCAAAAGGAG GGTAAAAATA AGTTAGAAGA TGGAAAGTCT 
GTTCATTTTG AATCCAGCAC TTCAGAGTCC TCAGTGCTAT CAAGTAGTAG TCCAGAAAGT 
ACCCTGGTGA AGACAGAGCC TAATGGGATA ACCATCCCTG GCATCTCATC AGATATGCCA 
GATAGTGCCC ACAAAACTCC TGCCTCAGAA GCAAAGTCAG AAACTGGGCA GCCTACCAAG 
GTTGGACGTT TTCAGGTGAC AACTACAGCA AACAAAGTAG GTCGTTTCTC TGTGTCAAGA 
ACTGAAGACA AGCTCATTGA GGCAAAGAAA GAGGGACCAA TAGCGTCTCC TCCTTTTATG 
GATTTGGAAC AAGGTGTTCT TCCAGCTGTG ATACCAAAGA AAGAAAAGCC TGAACTGTCA 
GAGCCTTCAC ATCTGAACGG ACCATCTTCT GACCTGGAGG CTGCCTTCCT GAGCAGGGAT 
GTGGATGATG GTTCAGGTAG TCCACACTCC CCCCATCAGC TGAGCTCAAA GAGCCTGCCT 
ATCCAGAATC TAAGTCAAAG CCTTAGTAAT TCATTCAACT CCTCTTACAT GAGTAGTGAC 
AATGAGTCAG ATATTGAAGA TGAAGATTTA AAATTAGAGC TGCGAAGACT ACGAGAAAAA 
CATCTTAAAG AGATTCAAGA CCTGCAGAGT CGCCAGAAGC ATGAAATTGA ATCTTTGTAT 
ACCAAACTGG GAAAGGTTCC CCCTGCTGTC ATTATCCCCC CAGCTGCCCC CCTTTCAGGG 
AGAAGAAGGC GACCCACTAA AAGCAAAGGC AGCAAGTCTA GTCGCAGCAG TTCCTTGGGG 
AATAAAAGTC CCCAGCTTTC AGGTAACCTG TCTGGTCAGA GTGCTGCTTC AGTTTTGCAC 
GCTCAGCAGA CCCTCCACCC TCCTGGCAAC ATCCCTGAGA CTGGGCCAAA TCAGCTGTTA 
CAGCCCCTTA AGCCATCTCC CTCCAGTGAC AACCTCTACT CAGCCTTTAC CAGTGATGGT 
GCCATTTCAG TCCCAAGCCT TTCTGCTCCA GGTCAAGGGA CCAGCAGCAC AAACACTGTC 
GGGGGAACAG TGAACAGCCA AGCTGCCCCA GCTCAGCCTC CTGCCATGAC ATCCAGCAGG 
AAGGGCACAT TCACAGATGA CCTACACAAG TTGGTAGACA ATTGGGCCCG AGATGCCATG 
AATCTCTCAG GCAGAAGAGG AAGCAAAGGA CACATGAATT ATGAGGGCCC TGGAATGGCA 
AGAAAGTTCT CCGCACCTGG TCAGCTGTGC ATCTCCATGA CCTCAAATCT GGGTGGTTCT 
GCCCCCATCT CTGCAGCATC AGCTACCTCT CTAGGTCACT TCACCAAGTC TATGTGCCCC 
CCACAGCAGT ACGGTTTTCC AGCTCCCCCC TTTGGCACTC AGTGGAGTGG GACGGGTGGC 
CCGGCACCAC AGCCACTTGG CCAGTTCCAG CCTGTGGGAA CTGCCTCCTT ACAGAATTTC 
AACATCAGCA ATTTGCAGAA ATCCATCAGC AACCCCCCAG GTTCCAACCT GCGGACCACT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_014108344.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963819.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X19, mRNA.

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CloneID	OCe43588
Clone ID Related Accession (Same CDS sequence)	XM_014108344.2 , XM_014108344.1
Accession Version	XM_014108344.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase WNK1 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014108344.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGGG AGTACAGCAG ACAGCCCCTC CACAACAGAC AGCGCAGTAT 
TCGCTTCCAC AGACATCAGC CTCCAGTGAG GCCACTATTG CACAACCAGT CAGTCAGTCT 
CAGGCTCCAC AGGTCTTGCC TCAAATATCA GCTGGAAAAC AGAGCACTCA GGGAGTCTCT 
CAAGTTGCTC CTCCAGAGCC AGTTCCAGTG GCACAGGCAC AGCCTACCAC TTTGGTCTCC 
TCTATAGACA GTGCACATTC AGATGTTGCT TCAGGAATGA GTGATGGCAA TGAGAATGTC 
CCGTCCTCCA GTGGAAGGCA TGAAGGGAGA GCTACAAAAC GACATTACCG AAAATCTGTA 
AGAAGTCGCT CTCGTCATGA AAAGACTTCG CGTCCAAAAT TAAGAATTTT GAATGTTTCA 
AATAAAGGAG ACCGAGTAGT AGAATGTCAA CTAGAGACTC ATAATCGGAA AATGGTTACA 
TTCAAATTTG ATTTAGATGG TGACAATCCT GAGGAGATAG CAACAATTAT GGTGAACAAT 
GACTTTATTC TAGCAATGGA GAGAGAGTCC TTTGTGGATC AAGTACGAGA AATTATTGAA 
AAGGCTGATG AAATGCTCAG TGAGGATGTC AGTGTGGAAC CAGAGGGTGA CCAGGGATTG 
GAGAGTCTGC AGGGAAAGGA TGACTATGGC TTTACAGGTT CTCAAAAATT GGAAGGAGAA 
TTCAAACAAC CAGTTCCTGC GTCTTCCATG CCACAGCAAA TAGGCATTCC TACCAGTTCT 
TTAACTCAAG TTGTTCATTC TGCTGGAAGA CGGTTTATAG TGAGTCCTGT GCCAGAAAGT 
CGATTACGAG AATCAAAAGT TTTCACTAGT GAAATACCAG ATACAGTTGC TGCCTCTACT 
CACAGCGCTG GAATGAACTT GTCTCACTCT GCTTCATCCC TTAGCCTACA GCAAGCCTTT 
TCTGAACTTA GACATGTGCA GATGACAGAA GGGCCCAGCA CAGCACCTCC CAACTTCAGT 
CATACAGGAC CAACATTTCC AGTTGTTCCT CCTTCCATGA GTAGCATTGC TGGAGTCCCA 
ACCACAGCAG TAGCCACACC TTCAGTATCA GTCCCTGCAA CTAGCAGCCC TCTAAATGAC 
AGTTCCACAT CAGTAATTCA ATCTGAGATT CCAGTGCCCA CTGAAACAGG GGTTGGTGGA 
GTTCCCACCT GCACAAGTGT AATGCCTTCA AGTGGTCTCC CTGTACCACC CATATCTGAA 
TCGCCAATAT TTTCCACTAT GGTTTCAAGC ATCACAGTAC CTACAGTTGT CTCAATATCA 
GCAACATCCC AGCCAGTTCA AGCTCCCACA TCTGGGAGCA CTGTTTCTAG CATAGGAACT 
TTGCCTTCTA TACCAGTTTC AACAGCTTTA GCCTCTGCAG TGGGCAGTGT TGCTATCCCA 
GGTGCTAAGC CTCCACCTGT ACTATCACAG CAGGCAGCAG GCAGTACTAC TGGGGCAGCC 
ACATTAACAT CCGTTCCTGC CATCACTCCG TTCCCAAGCA TAGCTTCACA GCCATCTCTT 
CAGCTTAGCA GCAGCACCTC TGCTCCAACT CTAGCTGAAA CAGTGGTGGT TAGTGCACAC 
TCACTAGATA AAACATCTCA TAGCAGTACA ACTGGATTGG CTTTGTCCCT GTCTGCATCA 
TCTTCCTCCC CTGGAGCAGG ATTATCTAGT TCTATTTCTC AGCCTGGTGG GGTGCATCCT 
TTGGTTACCC CATCAGCTAT GGCTTCTATT CCTGCCCTTC CTCAAACAGC AGGACCTACT 
TCTACACCTT TATTGCCCCA AGTACTTAGT ATCCCACCCT TGGTACAACC TGTTGCCAGT 
GTGCCTGCTG TGCAGCAGAC ACTAATTCAT AGTCAGCCTC AACCAGCTTT ACTTCCCAAC 
CAGCCCCACA CTCACTGTCC TGAAATAGAT GCTGACTTAC AGCCCAAAGC TCCTGGAATT 
GATGACATAA AGACTCTAGA GGAAAAGCTG CGGTCTCTCT TTAGTGAACC GAGCTCATCT 
GGAGCCCAAC ATACATCGGT CTCACTAGAG ACTTCACTGG TAGTAGAGAC CACTGTCACG 
CCAGGTATCC CAACCACTGC TATTGCACCA AGCAAGCTCA TGACATCTAC TACAAGTACA 
TGCTTACCAC CAACCAGTTT GCAATTAGGA ACATCTGGTT TGTCAGTTAT ACCAGTGGTC 
ACGCCTGGGC AGGTTTCTAC CCCAGTCAGC TATGTGTCAG CCCCAGGCAG CACTACATCA 
GGAGTGAAAG CTGGAACTGC TCCATCAAAG CCACCTTTGA CTAAAGCTCC AGTGCTGCCA 
GTGGGTACTG AACTTCCAGC TGGTACTCCA CCCAGCGAGC AGCTGCCACC TTTTCCAGGA 
CCTTCACTAA CTCAGTCCCA GCAACCTCTG GAAGATCTTG ATGCTCAGTT GAGAAGAACA 
CTTAGTCCAG AGACTATTGC GGTGACATCT ACAATTGGTC CTGTGTCCAT GGTGGCTCCA 
ACAGCAGTCA CAGAAGCAGG AGCACAGCCT CAGAAGGATG TTTCTCAGAT CACAGAAGGT 
CCTGTCCTAG CGACTAGTTC GGGAACTGGT GTTTTTAAGA TGGGAAGATT TCAGGTTTCA 
GTTGCAATGG ATGATGTTCA AAAGGAGGGT AAAAATAAGT TAGAAGATGG AAAGTCTGTT 
CATTTTGAAT CCAGCACTTC AGAGTCCTCA GTGCTATCAA GTAGTAGTCC AGAAAGTACC 
CTGGTGAAGA CAGAGCCTAA TGGGATAACC ATCCCTGGCA TCTCATCAGA TATGCCAGAT 
AGTGCCCACA AAACTCCTGC CTCAGAAGCA AAGTCAGAAA CTGGGCAGCC TACCAAGGTT 
GGACGTTTTC AGGTGACAAC TACAGCAAAC AAAGTAGGTC GTTTCTCTGT GTCAAGAACT 
GAAGACAAGC TCATTGAGGC AAAGAAAGAG GGACCAATAG CGTCTCCTCC TTTTATGGAT 
TTGGAACAAG GTGTTCTTCC AGCTGTGATA CCAAAGAAAG AAAAGCCTGA ACTGTCAGAG 
CCTTCACATC TGAACGGACC ATCTTCTGAC CTGGAGGCTG CCTTCCTGAG CAGGGATGTG 
GATGATGGTT CAGGTAGTCC ACACTCCCCC CATCAGCTGA GCTCAAAGAG CCTGCCTATC 
CAGAATCTAA GTCAAAGCCT TAGTAATTCA TTCAACTCCT CTTACATGAG TAGTGACAAT 
GAGTCAGATA TTGAAGATGA AGATTTAAAA TTAGAGCTGC GAAGACTACG AGAAAAACAT 
CTTAAAGAGA TTCAAGACCT GCAGAGTCGC CAGAAGCATG AAATTGAATC TTTGTATACC 
AAACTGGGAA AGGTTCCCCC TGCTGTCATT ATCCCCCCAG CTGCCCCCCT TTCAGGGAGA 
AGAAGGCGAC CCACTAAAAG CAAAGGCAGC AAGTCTAGTC GCAGCAGTTC CTTGGGGAAT 
AAAAGTCCCC AGCTTTCAGG TAACCTGTCT GGTCAGAGTG CTGCTTCAGT TTTGCACGCT 
CAGCAGACCC TCCACCCTCC TGGCAACATC CCTGAGACTG GGCCAAATCA GCTGTTACAG 
CCCCTTAAGC CATCTCCCTC CAGTGACAAC CTCTACTCAG CCTTTACCAG TGATGGTGCC 
ATTTCAGTCC CAAGCCTTTC TGCTCCAGGT CAAGGGACCA GCAGCACAAA CACTGTCGGG 
GGAACAGTGA ACAGCCAAGC TGCCCCAGCT CAGCCTCCTG CCATGACATC CAGCAGGAAG 
GGCACATTCA CAGATGACCT ACACAAGTTG GTAGACAATT GGGCCCGAGA TGCCATGAAT 
CTCTCAGGCA GAAGAGGAAG CAAAGGACAC ATGAATTATG AGGGCCCTGG AATGGCAAGA 
AAGTTCTCCG CACCTGGTCA GCTGTGCATC TCCATGACCT CAAATCTGGG TGGTTCTGCC 
CCCATCTCTG CAGCATCAGC TACCTCTCTA GGTCACTTCA CCAAGTCTAT GTGCCCCCCA 
CAGCAGTACG GTTTTCCAGC TCCCCCCTTT GGCACTCAGT GGAGTGGGAC GGGTGGCCCG 
GCACCACAGC CACTTGGCCA GTTCCAGCCT GTGGGAACTG CCTCCTTACA GAATTTCAAC 
ATCAGCAATT TGCAGAAATC CATCAGCAAC CCCCCAGGTT CCAACCTGCG GACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963819.1
CDS	994..7413
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVGQSQGQPSSSSLTGIPSSLPIQHSQQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQSTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_014108344.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108344.2

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGGG AGTACAGCAG ACAGCCCCTC CACAACAGAC AGCGCAGTAT 
TCGCTTCCAC AGACATCAGC CTCCAGTGAG GCCACTATTG CACAACCAGT CAGTCAGTCT 
CAGGCTCCAC AGGTCTTGCC TCAAATATCA GCTGGAAAAC AGAGCACTCA GGGAGTCTCT 
CAAGTTGCTC CTCCAGAGCC AGTTCCAGTG GCACAGGCAC AGCCTACCAC TTTGGTCTCC 
TCTATAGACA GTGCACATTC AGATGTTGCT TCAGGAATGA GTGATGGCAA TGAGAATGTC 
CCGTCCTCCA GTGGAAGGCA TGAAGGGAGA GCTACAAAAC GACATTACCG AAAATCTGTA 
AGAAGTCGCT CTCGTCATGA AAAGACTTCG CGTCCAAAAT TAAGAATTTT GAATGTTTCA 
AATAAAGGAG ACCGAGTAGT AGAATGTCAA CTAGAGACTC ATAATCGGAA AATGGTTACA 
TTCAAATTTG ATTTAGATGG TGACAATCCT GAGGAGATAG CAACAATTAT GGTGAACAAT 
GACTTTATTC TAGCAATGGA GAGAGAGTCC TTTGTGGATC AAGTACGAGA AATTATTGAA 
AAGGCTGATG AAATGCTCAG TGAGGATGTC AGTGTGGAAC CAGAGGGTGA CCAGGGATTG 
GAGAGTCTGC AGGGAAAGGA TGACTATGGC TTTACAGGTT CTCAAAAATT GGAAGGAGAA 
TTCAAACAAC CAGTTCCTGC GTCTTCCATG CCACAGCAAA TAGGCATTCC TACCAGTTCT 
TTAACTCAAG TTGTTCATTC TGCTGGAAGA CGGTTTATAG TGAGTCCTGT GCCAGAAAGT 
CGATTACGAG AATCAAAAGT TTTCACTAGT GAAATACCAG ATACAGTTGC TGCCTCTACT 
CACAGCGCTG GAATGAACTT GTCTCACTCT GCTTCATCCC TTAGCCTACA GCAAGCCTTT 
TCTGAACTTA GACATGTGCA GATGACAGAA GGGCCCAGCA CAGCACCTCC CAACTTCAGT 
CATACAGGAC CAACATTTCC AGTTGTTCCT CCTTCCATGA GTAGCATTGC TGGAGTCCCA 
ACCACAGCAG TAGCCACACC TTCAGTATCA GTCCCTGCAA CTAGCAGCCC TCTAAATGAC 
AGTTCCACAT CAGTAATTCA ATCTGAGATT CCAGTGCCCA CTGAAACAGG GGTTGGTGGA 
GTTCCCACCT GCACAAGTGT AATGCCTTCA AGTGGTCTCC CTGTACCACC CATATCTGAA 
TCGCCAATAT TTTCCACTAT GGTTTCAAGC ATCACAGTAC CTACAGTTGT CTCAATATCA 
GCAACATCCC AGCCAGTTCA AGCTCCCACA TCTGGGAGCA CTGTTTCTAG CATAGGAACT 
TTGCCTTCTA TACCAGTTTC AACAGCTTTA GCCTCTGCAG TGGGCAGTGT TGCTATCCCA 
GGTGCTAAGC CTCCACCTGT ACTATCACAG CAGGCAGCAG GCAGTACTAC TGGGGCAGCC 
ACATTAACAT CCGTTCCTGC CATCACTCCG TTCCCAAGCA TAGCTTCACA GCCATCTCTT 
CAGCTTAGCA GCAGCACCTC TGCTCCAACT CTAGCTGAAA CAGTGGTGGT TAGTGCACAC 
TCACTAGATA AAACATCTCA TAGCAGTACA ACTGGATTGG CTTTGTCCCT GTCTGCATCA 
TCTTCCTCCC CTGGAGCAGG ATTATCTAGT TCTATTTCTC AGCCTGGTGG GGTGCATCCT 
TTGGTTACCC CATCAGCTAT GGCTTCTATT CCTGCCCTTC CTCAAACAGC AGGACCTACT 
TCTACACCTT TATTGCCCCA AGTACTTAGT ATCCCACCCT TGGTACAACC TGTTGCCAGT 
GTGCCTGCTG TGCAGCAGAC ACTAATTCAT AGTCAGCCTC AACCAGCTTT ACTTCCCAAC 
CAGCCCCACA CTCACTGTCC TGAAATAGAT GCTGACTTAC AGCCCAAAGC TCCTGGAATT 
GATGACATAA AGACTCTAGA GGAAAAGCTG CGGTCTCTCT TTAGTGAACC GAGCTCATCT 
GGAGCCCAAC ATACATCGGT CTCACTAGAG ACTTCACTGG TAGTAGAGAC CACTGTCACG 
CCAGGTATCC CAACCACTGC TATTGCACCA AGCAAGCTCA TGACATCTAC TACAAGTACA 
TGCTTACCAC CAACCAGTTT GCAATTAGGA ACATCTGGTT TGTCAGTTAT ACCAGTGGTC 
ACGCCTGGGC AGGTTTCTAC CCCAGTCAGC TATGTGTCAG CCCCAGGCAG CACTACATCA 
GGAGTGAAAG CTGGAACTGC TCCATCAAAG CCACCTTTGA CTAAAGCTCC AGTGCTGCCA 
GTGGGTACTG AACTTCCAGC TGGTACTCCA CCCAGCGAGC AGCTGCCACC TTTTCCAGGA 
CCTTCACTAA CTCAGTCCCA GCAACCTCTG GAAGATCTTG ATGCTCAGTT GAGAAGAACA 
CTTAGTCCAG AGACTATTGC GGTGACATCT ACAATTGGTC CTGTGTCCAT GGTGGCTCCA 
ACAGCAGTCA CAGAAGCAGG AGCACAGCCT CAGAAGGATG TTTCTCAGAT CACAGAAGGT 
CCTGTCCTAG CGACTAGTTC GGGAACTGGT GTTTTTAAGA TGGGAAGATT TCAGGTTTCA 
GTTGCAATGG ATGATGTTCA AAAGGAGGGT AAAAATAAGT TAGAAGATGG AAAGTCTGTT 
CATTTTGAAT CCAGCACTTC AGAGTCCTCA GTGCTATCAA GTAGTAGTCC AGAAAGTACC 
CTGGTGAAGA CAGAGCCTAA TGGGATAACC ATCCCTGGCA TCTCATCAGA TATGCCAGAT 
AGTGCCCACA AAACTCCTGC CTCAGAAGCA AAGTCAGAAA CTGGGCAGCC TACCAAGGTT 
GGACGTTTTC AGGTGACAAC TACAGCAAAC AAAGTAGGTC GTTTCTCTGT GTCAAGAACT 
GAAGACAAGC TCATTGAGGC AAAGAAAGAG GGACCAATAG CGTCTCCTCC TTTTATGGAT 
TTGGAACAAG GTGTTCTTCC AGCTGTGATA CCAAAGAAAG AAAAGCCTGA ACTGTCAGAG 
CCTTCACATC TGAACGGACC ATCTTCTGAC CTGGAGGCTG CCTTCCTGAG CAGGGATGTG 
GATGATGGTT CAGGTAGTCC ACACTCCCCC CATCAGCTGA GCTCAAAGAG CCTGCCTATC 
CAGAATCTAA GTCAAAGCCT TAGTAATTCA TTCAACTCCT CTTACATGAG TAGTGACAAT 
GAGTCAGATA TTGAAGATGA AGATTTAAAA TTAGAGCTGC GAAGACTACG AGAAAAACAT 
CTTAAAGAGA TTCAAGACCT GCAGAGTCGC CAGAAGCATG AAATTGAATC TTTGTATACC 
AAACTGGGAA AGGTTCCCCC TGCTGTCATT ATCCCCCCAG CTGCCCCCCT TTCAGGGAGA 
AGAAGGCGAC CCACTAAAAG CAAAGGCAGC AAGTCTAGTC GCAGCAGTTC CTTGGGGAAT 
AAAAGTCCCC AGCTTTCAGG TAACCTGTCT GGTCAGAGTG CTGCTTCAGT TTTGCACGCT 
CAGCAGACCC TCCACCCTCC TGGCAACATC CCTGAGACTG GGCCAAATCA GCTGTTACAG 
CCCCTTAAGC CATCTCCCTC CAGTGACAAC CTCTACTCAG CCTTTACCAG TGATGGTGCC 
ATTTCAGTCC CAAGCCTTTC TGCTCCAGGT CAAGGGACCA GCAGCACAAA CACTGTCGGG 
GGAACAGTGA ACAGCCAAGC TGCCCCAGCT CAGCCTCCTG CCATGACATC CAGCAGGAAG 
GGCACATTCA CAGATGACCT ACACAAGTTG GTAGACAATT GGGCCCGAGA TGCCATGAAT 
CTCTCAGGCA GAAGAGGAAG CAAAGGACAC ATGAATTATG AGGGCCCTGG AATGGCAAGA 
AAGTTCTCCG CACCTGGTCA GCTGTGCATC TCCATGACCT CAAATCTGGG TGGTTCTGCC 
CCCATCTCTG CAGCATCAGC TACCTCTCTA GGTCACTTCA CCAAGTCTAT GTGCCCCCCA 
CAGCAGTACG GTTTTCCAGC TCCCCCCTTT GGCACTCAGT GGAGTGGGAC GGGTGGCCCG 
GCACCACAGC CACTTGGCCA GTTCCAGCCT GTGGGAACTG CCTCCTTACA GAATTTCAAC 
ATCAGCAATT TGCAGAAATC CATCAGCAAC CCCCCAGGTT CCAACCTGCG GACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_014108344.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963819.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X20, mRNA.

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CloneID	OCe43588
Clone ID Related Accession (Same CDS sequence)	XM_014108344.2 , XM_014108344.1
Accession Version	XM_014108344.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase WNK1 isoform X20
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGGG AGTACAGCAG ACAGCCCCTC CACAACAGAC AGCGCAGTAT 
TCGCTTCCAC AGACATCAGC CTCCAGTGAG GCCACTATTG CACAACCAGT CAGTCAGTCT 
CAGGCTCCAC AGGTCTTGCC TCAAATATCA GCTGGAAAAC AGAGCACTCA GGGAGTCTCT 
CAAGTTGCTC CTCCAGAGCC AGTTCCAGTG GCACAGGCAC AGCCTACCAC TTTGGTCTCC 
TCTATAGACA GTGCACATTC AGATGTTGCT TCAGGAATGA GTGATGGCAA TGAGAATGTC 
CCGTCCTCCA GTGGAAGGCA TGAAGGGAGA GCTACAAAAC GACATTACCG AAAATCTGTA 
AGAAGTCGCT CTCGTCATGA AAAGACTTCG CGTCCAAAAT TAAGAATTTT GAATGTTTCA 
AATAAAGGAG ACCGAGTAGT AGAATGTCAA CTAGAGACTC ATAATCGGAA AATGGTTACA 
TTCAAATTTG ATTTAGATGG TGACAATCCT GAGGAGATAG CAACAATTAT GGTGAACAAT 
GACTTTATTC TAGCAATGGA GAGAGAGTCC TTTGTGGATC AAGTACGAGA AATTATTGAA 
AAGGCTGATG AAATGCTCAG TGAGGATGTC AGTGTGGAAC CAGAGGGTGA CCAGGGATTG 
GAGAGTCTGC AGGGAAAGGA TGACTATGGC TTTACAGGTT CTCAAAAATT GGAAGGAGAA 
TTCAAACAAC CAGTTCCTGC GTCTTCCATG CCACAGCAAA TAGGCATTCC TACCAGTTCT 
TTAACTCAAG TTGTTCATTC TGCTGGAAGA CGGTTTATAG TGAGTCCTGT GCCAGAAAGT 
CGATTACGAG AATCAAAAGT TTTCACTAGT GAAATACCAG ATACAGTTGC TGCCTCTACT 
CACAGCGCTG GAATGAACTT GTCTCACTCT GCTTCATCCC TTAGCCTACA GCAAGCCTTT 
TCTGAACTTA GACATGTGCA GATGACAGAA GGGCCCAGCA CAGCACCTCC CAACTTCAGT 
CATACAGGAC CAACATTTCC AGTTGTTCCT CCTTCCATGA GTAGCATTGC TGGAGTCCCA 
ACCACAGCAG TAGCCACACC TTCAGTATCA GTCCCTGCAA CTAGCAGCCC TCTAAATGAC 
AGTTCCACAT CAGTAATTCA ATCTGAGATT CCAGTGCCCA CTGAAACAGG GGTTGGTGGA 
GTTCCCACCT GCACAAGTGT AATGCCTTCA AGTGGTCTCC CTGTACCACC CATATCTGAA 
TCGCCAATAT TTTCCACTAT GGTTTCAAGC ATCACAGTAC CTACAGTTGT CTCAATATCA 
GCAACATCCC AGCCAGTTCA AGCTCCCACA TCTGGGAGCA CTGTTTCTAG CATAGGAACT 
TTGCCTTCTA TACCAGTTTC AACAGCTTTA GCCTCTGCAG TGGGCAGTGT TGCTATCCCA 
GGTGCTAAGC CTCCACCTGT ACTATCACAG CAGGCAGCAG GCAGTACTAC TGGGGCAGCC 
ACATTAACAT CCGTTCCTGC CATCACTCCG TTCCCAAGCA TAGCTTCACA GCCATCTCTT 
CAGCTTAGCA GCAGCACCTC TGCTCCAACT CTAGCTGAAA CAGTGGTGGT TAGTGCACAC 
TCACTAGATA AAACATCTCA TAGCAGTACA ACTGGATTGG CTTTGTCCCT GTCTGCATCA 
TCTTCCTCCC CTGGAGCAGG ATTATCTAGT TCTATTTCTC AGCCTGGTGG GGTGCATCCT 
TTGGTTACCC CATCAGCTAT GGCTTCTATT CCTGCCCTTC CTCAAACAGC AGGACCTACT 
TCTACACCTT TATTGCCCCA AGTACTTAGT ATCCCACCCT TGGTACAACC TGTTGCCAGT 
GTGCCTGCTG TGCAGCAGAC ACTAATTCAT AGTCAGCCTC AACCAGCTTT ACTTCCCAAC 
CAGCCCCACA CTCACTGTCC TGAAATAGAT GCTGACTTAC AGCCCAAAGC TCCTGGAATT 
GATGACATAA AGACTCTAGA GGAAAAGCTG CGGTCTCTCT TTAGTGAACC GAGCTCATCT 
GGAGCCCAAC ATACATCGGT CTCACTAGAG ACTTCACTGG TAGTAGAGAC CACTGTCACG 
CCAGGTATCC CAACCACTGC TATTGCACCA AGCAAGCTCA TGACATCTAC TACAAGTACA 
TGCTTACCAC CAACCAGTTT GCAATTAGGA ACATCTGGTT TGTCAGTTAT ACCAGTGGTC 
ACGCCTGGGC AGGTTTCTAC CCCAGTCAGC TATGTGTCAG CCCCAGGCAG CACTACATCA 
GGAGTGAAAG CTGGAACTGC TCCATCAAAG CCACCTTTGA CTAAAGCTCC AGTGCTGCCA 
GTGGGTACTG AACTTCCAGC TGGTACTCCA CCCAGCGAGC AGCTGCCACC TTTTCCAGGA 
CCTTCACTAA CTCAGTCCCA GCAACCTCTG GAAGATCTTG ATGCTCAGTT GAGAAGAACA 
CTTAGTCCAG AGACTATTGC GGTGACATCT ACAATTGGTC CTGTGTCCAT GGTGGCTCCA 
ACAGCAGTCA CAGAAGCAGG AGCACAGCCT CAGAAGGATG TTTCTCAGAT CACAGAAGGT 
CCTGTCCTAG CGACTAGTTC GGGAACTGGT GTTTTTAAGA TGGGAAGATT TCAGGTTTCA 
GTTGCAATGG ATGATGTTCA AAAGGAGGGT AAAAATAAGT TAGAAGATGG AAAGTCTGTT 
CATTTTGAAT CCAGCACTTC AGAGTCCTCA GTGCTATCAA GTAGTAGTCC AGAAAGTACC 
CTGGTGAAGA CAGAGCCTAA TGGGATAACC ATCCCTGGCA TCTCATCAGA TATGCCAGAT 
AGTGCCCACA AAACTCCTGC CTCAGAAGCA AAGTCAGAAA CTGGGCAGCC TACCAAGGTT 
GGACGTTTTC AGGTGACAAC TACAGCAAAC AAAGTAGGTC GTTTCTCTGT GTCAAGAACT 
GAAGACAAGC TCATTGAGGC AAAGAAAGAG GGACCAATAG CGTCTCCTCC TTTTATGGAT 
TTGGAACAAG GTGTTCTTCC AGCTGTGATA CCAAAGAAAG AAAAGCCTGA ACTGTCAGAG 
CCTTCACATC TGAACGGACC ATCTTCTGAC CTGGAGGCTG CCTTCCTGAG CAGGGATGTG 
GATGATGGTT CAGGTAGTCC ACACTCCCCC CATCAGCTGA GCTCAAAGAG CCTGCCTATC 
CAGAATCTAA GTCAAAGCCT TAGTAATTCA TTCAACTCCT CTTACATGAG TAGTGACAAT 
GAGTCAGATA TTGAAGATGA AGATTTAAAA TTAGAGCTGC GAAGACTACG AGAAAAACAT 
CTTAAAGAGA TTCAAGACCT GCAGAGTCGC CAGAAGCATG AAATTGAATC TTTGTATACC 
AAACTGGGAA AGGTTCCCCC TGCTGTCATT ATCCCCCCAG CTGCCCCCCT TTCAGGGAGA 
AGAAGGCGAC CCACTAAAAG CAAAGGCAGC AAGTCTAGTC GCAGCAGTTC CTTGGGGAAT 
AAAAGTCCCC AGCTTTCAGG TAACCTGTCT GGTCAGAGTG CTGCTTCAGT TTTGCACGCT 
CAGCAGACCC TCCACCCTCC TGGCAACATC CCTGAGACTG GGCCAAATCA GCTGTTACAG 
CCCCTTAAGC CATCTCCCTC CAGTGACAAC CTCTACTCAG CCTTTACCAG TGATGGTGCC 
ATTTCAGTCC CAAGCCTTTC TGCTCCAGGT CAAGGGACCA GCAGCACAAA CACTGTCGGG 
GGAACAGTGA ACAGCCAAGC TGCCCCAGCT CAGCCTCCTG CCATGACATC CAGCAGGAAG 
GGCACATTCA CAGATGACCT ACACAAGTTG GTAGACAATT GGGCCCGAGA TGCCATGAAT 
CTCTCAGGCA GAAGAGGAAG CAAAGGACAC ATGAATTATG AGGGCCCTGG AATGGCAAGA 
AAGTTCTCCG CACCTGGTCA GCTGTGCATC TCCATGACCT CAAATCTGGG TGGTTCTGCC 
CCCATCTCTG CAGCATCAGC TACCTCTCTA GGTCACTTCA CCAAGTCTAT GTGCCCCCCA 
CAGCAGTACG GTTTTCCAGC TCCCCCCTTT GGCACTCAGT GGAGTGGGAC GGGTGGCCCG 
GCACCACAGC CACTTGGCCA GTTCCAGCCT GTGGGAACTG CCTCCTTACA GAATTTCAAC 
ATCAGCAATT TGCAGAAATC CATCAGCAAC CCCCCAGGTT CCAACCTGCG GACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963819.1
CDS	99..6518
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVGQSQGQPSSSSLTGIPSSLPIQHSQQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQSTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_014108344.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108344.1

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGGGG 
CAAAGCCAGG GTCAGCCATC CTCAAGTAGC TTAACAGGGA TTCCATCTTC CCTCCCCATA 
CAACATTCTC AGCAGCAGGG AGTACAGCAG ACAGCCCCTC CACAACAGAC AGCGCAGTAT 
TCGCTTCCAC AGACATCAGC CTCCAGTGAG GCCACTATTG CACAACCAGT CAGTCAGTCT 
CAGGCTCCAC AGGTCTTGCC TCAAATATCA GCTGGAAAAC AGAGCACTCA GGGAGTCTCT 
CAAGTTGCTC CTCCAGAGCC AGTTCCAGTG GCACAGGCAC AGCCTACCAC TTTGGTCTCC 
TCTATAGACA GTGCACATTC AGATGTTGCT TCAGGAATGA GTGATGGCAA TGAGAATGTC 
CCGTCCTCCA GTGGAAGGCA TGAAGGGAGA GCTACAAAAC GACATTACCG AAAATCTGTA 
AGAAGTCGCT CTCGTCATGA AAAGACTTCG CGTCCAAAAT TAAGAATTTT GAATGTTTCA 
AATAAAGGAG ACCGAGTAGT AGAATGTCAA CTAGAGACTC ATAATCGGAA AATGGTTACA 
TTCAAATTTG ATTTAGATGG TGACAATCCT GAGGAGATAG CAACAATTAT GGTGAACAAT 
GACTTTATTC TAGCAATGGA GAGAGAGTCC TTTGTGGATC AAGTACGAGA AATTATTGAA 
AAGGCTGATG AAATGCTCAG TGAGGATGTC AGTGTGGAAC CAGAGGGTGA CCAGGGATTG 
GAGAGTCTGC AGGGAAAGGA TGACTATGGC TTTACAGGTT CTCAAAAATT GGAAGGAGAA 
TTCAAACAAC CAGTTCCTGC GTCTTCCATG CCACAGCAAA TAGGCATTCC TACCAGTTCT 
TTAACTCAAG TTGTTCATTC TGCTGGAAGA CGGTTTATAG TGAGTCCTGT GCCAGAAAGT 
CGATTACGAG AATCAAAAGT TTTCACTAGT GAAATACCAG ATACAGTTGC TGCCTCTACT 
CACAGCGCTG GAATGAACTT GTCTCACTCT GCTTCATCCC TTAGCCTACA GCAAGCCTTT 
TCTGAACTTA GACATGTGCA GATGACAGAA GGGCCCAGCA CAGCACCTCC CAACTTCAGT 
CATACAGGAC CAACATTTCC AGTTGTTCCT CCTTCCATGA GTAGCATTGC TGGAGTCCCA 
ACCACAGCAG TAGCCACACC TTCAGTATCA GTCCCTGCAA CTAGCAGCCC TCTAAATGAC 
AGTTCCACAT CAGTAATTCA ATCTGAGATT CCAGTGCCCA CTGAAACAGG GGTTGGTGGA 
GTTCCCACCT GCACAAGTGT AATGCCTTCA AGTGGTCTCC CTGTACCACC CATATCTGAA 
TCGCCAATAT TTTCCACTAT GGTTTCAAGC ATCACAGTAC CTACAGTTGT CTCAATATCA 
GCAACATCCC AGCCAGTTCA AGCTCCCACA TCTGGGAGCA CTGTTTCTAG CATAGGAACT 
TTGCCTTCTA TACCAGTTTC AACAGCTTTA GCCTCTGCAG TGGGCAGTGT TGCTATCCCA 
GGTGCTAAGC CTCCACCTGT ACTATCACAG CAGGCAGCAG GCAGTACTAC TGGGGCAGCC 
ACATTAACAT CCGTTCCTGC CATCACTCCG TTCCCAAGCA TAGCTTCACA GCCATCTCTT 
CAGCTTAGCA GCAGCACCTC TGCTCCAACT CTAGCTGAAA CAGTGGTGGT TAGTGCACAC 
TCACTAGATA AAACATCTCA TAGCAGTACA ACTGGATTGG CTTTGTCCCT GTCTGCATCA 
TCTTCCTCCC CTGGAGCAGG ATTATCTAGT TCTATTTCTC AGCCTGGTGG GGTGCATCCT 
TTGGTTACCC CATCAGCTAT GGCTTCTATT CCTGCCCTTC CTCAAACAGC AGGACCTACT 
TCTACACCTT TATTGCCCCA AGTACTTAGT ATCCCACCCT TGGTACAACC TGTTGCCAGT 
GTGCCTGCTG TGCAGCAGAC ACTAATTCAT AGTCAGCCTC AACCAGCTTT ACTTCCCAAC 
CAGCCCCACA CTCACTGTCC TGAAATAGAT GCTGACTTAC AGCCCAAAGC TCCTGGAATT 
GATGACATAA AGACTCTAGA GGAAAAGCTG CGGTCTCTCT TTAGTGAACC GAGCTCATCT 
GGAGCCCAAC ATACATCGGT CTCACTAGAG ACTTCACTGG TAGTAGAGAC CACTGTCACG 
CCAGGTATCC CAACCACTGC TATTGCACCA AGCAAGCTCA TGACATCTAC TACAAGTACA 
TGCTTACCAC CAACCAGTTT GCAATTAGGA ACATCTGGTT TGTCAGTTAT ACCAGTGGTC 
ACGCCTGGGC AGGTTTCTAC CCCAGTCAGC TATGTGTCAG CCCCAGGCAG CACTACATCA 
GGAGTGAAAG CTGGAACTGC TCCATCAAAG CCACCTTTGA CTAAAGCTCC AGTGCTGCCA 
GTGGGTACTG AACTTCCAGC TGGTACTCCA CCCAGCGAGC AGCTGCCACC TTTTCCAGGA 
CCTTCACTAA CTCAGTCCCA GCAACCTCTG GAAGATCTTG ATGCTCAGTT GAGAAGAACA 
CTTAGTCCAG AGACTATTGC GGTGACATCT ACAATTGGTC CTGTGTCCAT GGTGGCTCCA 
ACAGCAGTCA CAGAAGCAGG AGCACAGCCT CAGAAGGATG TTTCTCAGAT CACAGAAGGT 
CCTGTCCTAG CGACTAGTTC GGGAACTGGT GTTTTTAAGA TGGGAAGATT TCAGGTTTCA 
GTTGCAATGG ATGATGTTCA AAAGGAGGGT AAAAATAAGT TAGAAGATGG AAAGTCTGTT 
CATTTTGAAT CCAGCACTTC AGAGTCCTCA GTGCTATCAA GTAGTAGTCC AGAAAGTACC 
CTGGTGAAGA CAGAGCCTAA TGGGATAACC ATCCCTGGCA TCTCATCAGA TATGCCAGAT 
AGTGCCCACA AAACTCCTGC CTCAGAAGCA AAGTCAGAAA CTGGGCAGCC TACCAAGGTT 
GGACGTTTTC AGGTGACAAC TACAGCAAAC AAAGTAGGTC GTTTCTCTGT GTCAAGAACT 
GAAGACAAGC TCATTGAGGC AAAGAAAGAG GGACCAATAG CGTCTCCTCC TTTTATGGAT 
TTGGAACAAG GTGTTCTTCC AGCTGTGATA CCAAAGAAAG AAAAGCCTGA ACTGTCAGAG 
CCTTCACATC TGAACGGACC ATCTTCTGAC CTGGAGGCTG CCTTCCTGAG CAGGGATGTG 
GATGATGGTT CAGGTAGTCC ACACTCCCCC CATCAGCTGA GCTCAAAGAG CCTGCCTATC 
CAGAATCTAA GTCAAAGCCT TAGTAATTCA TTCAACTCCT CTTACATGAG TAGTGACAAT 
GAGTCAGATA TTGAAGATGA AGATTTAAAA TTAGAGCTGC GAAGACTACG AGAAAAACAT 
CTTAAAGAGA TTCAAGACCT GCAGAGTCGC CAGAAGCATG AAATTGAATC TTTGTATACC 
AAACTGGGAA AGGTTCCCCC TGCTGTCATT ATCCCCCCAG CTGCCCCCCT TTCAGGGAGA 
AGAAGGCGAC CCACTAAAAG CAAAGGCAGC AAGTCTAGTC GCAGCAGTTC CTTGGGGAAT 
AAAAGTCCCC AGCTTTCAGG TAACCTGTCT GGTCAGAGTG CTGCTTCAGT TTTGCACGCT 
CAGCAGACCC TCCACCCTCC TGGCAACATC CCTGAGACTG GGCCAAATCA GCTGTTACAG 
CCCCTTAAGC CATCTCCCTC CAGTGACAAC CTCTACTCAG CCTTTACCAG TGATGGTGCC 
ATTTCAGTCC CAAGCCTTTC TGCTCCAGGT CAAGGGACCA GCAGCACAAA CACTGTCGGG 
GGAACAGTGA ACAGCCAAGC TGCCCCAGCT CAGCCTCCTG CCATGACATC CAGCAGGAAG 
GGCACATTCA CAGATGACCT ACACAAGTTG GTAGACAATT GGGCCCGAGA TGCCATGAAT 
CTCTCAGGCA GAAGAGGAAG CAAAGGACAC ATGAATTATG AGGGCCCTGG AATGGCAAGA 
AAGTTCTCCG CACCTGGTCA GCTGTGCATC TCCATGACCT CAAATCTGGG TGGTTCTGCC 
CCCATCTCTG CAGCATCAGC TACCTCTCTA GGTCACTTCA CCAAGTCTAT GTGCCCCCCA 
CAGCAGTACG GTTTTCCAGC TCCCCCCTTT GGCACTCAGT GGAGTGGGAC GGGTGGCCCG 
GCACCACAGC CACTTGGCCA GTTCCAGCCT GTGGGAACTG CCTCCTTACA GAATTTCAAC 
ATCAGCAATT TGCAGAAATC CATCAGCAAC CCCCCAGGTT CCAACCTGCG GACCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_014108345.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963820.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X20, mRNA.

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CloneID	OCe43589
Clone ID Related Accession (Same CDS sequence)	XM_014108345.2 , XM_014108345.1
Accession Version	XM_014108345.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase WNK1 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014108345.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGCAG 
CAGGGAGTAC AGCAGACAGC CCCTCCACAA CAGACAGCGC AGTATTCGCT TCCACAGACA 
TCAGCCTCCA GTGAGGCCAC TATTGCACAA CCAGTCAGTC AGTCTCAGGC TCCACAGGTC 
TTGCCTCAAA TATCAGCTGG AAAACAGAGC ACTCAGGGAG TCTCTCAAGT TGCTCCTCCA 
GAGCCAGTTC CAGTGGCACA GGCACAGCCT ACCACTTTGG TCTCCTCTAT AGACAGTGCA 
CATTCAGATG TTGCTTCAGG AATGAGTGAT GGCAATGAGA ATGTCCCGTC CTCCAGTGGA 
AGGCATGAAG GGAGAGCTAC AAAACGACAT TACCGAAAAT CTGTAAGAAG TCGCTCTCGT 
CATGAAAAGA CTTCGCGTCC AAAATTAAGA ATTTTGAATG TTTCAAATAA AGGAGACCGA 
GTAGTAGAAT GTCAACTAGA GACTCATAAT CGGAAAATGG TTACATTCAA ATTTGATTTA 
GATGGTGACA ATCCTGAGGA GATAGCAACA ATTATGGTGA ACAATGACTT TATTCTAGCA 
ATGGAGAGAG AGTCCTTTGT GGATCAAGTA CGAGAAATTA TTGAAAAGGC TGATGAAATG 
CTCAGTGAGG ATGTCAGTGT GGAACCAGAG GGTGACCAGG GATTGGAGAG TCTGCAGGGA 
AAGGATGACT ATGGCTTTAC AGGTTCTCAA AAATTGGAAG GAGAATTCAA ACAACCAGTT 
CCTGCGTCTT CCATGCCACA GCAAATAGGC ATTCCTACCA GTTCTTTAAC TCAAGTTGTT 
CATTCTGCTG GAAGACGGTT TATAGTGAGT CCTGTGCCAG AAAGTCGATT ACGAGAATCA 
AAAGTTTTCA CTAGTGAAAT ACCAGATACA GTTGCTGCCT CTACTCACAG CGCTGGAATG 
AACTTGTCTC ACTCTGCTTC ATCCCTTAGC CTACAGCAAG CCTTTTCTGA ACTTAGACAT 
GTGCAGATGA CAGAAGGGCC CAGCACAGCA CCTCCCAACT TCAGTCATAC AGGACCAACA 
TTTCCAGTTG TTCCTCCTTC CATGAGTAGC ATTGCTGGAG TCCCAACCAC AGCAGTAGCC 
ACACCTTCAG TATCAGTCCC TGCAACTAGC AGCCCTCTAA ATGACAGTTC CACATCAGTA 
ATTCAATCTG AGATTCCAGT GCCCACTGAA ACAGGGGTTG GTGGAGTTCC CACCTGCACA 
AGTGTAATGC CTTCAAGTGG TCTCCCTGTA CCACCCATAT CTGAATCGCC AATATTTTCC 
ACTATGGTTT CAAGCATCAC AGTACCTACA GTTGTCTCAA TATCAGCAAC ATCCCAGCCA 
GTTCAAGCTC CCACATCTGG GAGCACTGTT TCTAGCATAG GAACTTTGCC TTCTATACCA 
GTTTCAACAG CTTTAGCCTC TGCAGTGGGC AGTGTTGCTA TCCCAGGTGC TAAGCCTCCA 
CCTGTACTAT CACAGCAGGC AGCAGGCAGT ACTACTGGGG CAGCCACATT AACATCCGTT 
CCTGCCATCA CTCCGTTCCC AAGCATAGCT TCACAGCCAT CTCTTCAGCT TAGCAGCAGC 
ACCTCTGCTC CAACTCTAGC TGAAACAGTG GTGGTTAGTG CACACTCACT AGATAAAACA 
TCTCATAGCA GTACAACTGG ATTGGCTTTG TCCCTGTCTG CATCATCTTC CTCCCCTGGA 
GCAGGATTAT CTAGTTCTAT TTCTCAGCCT GGTGGGGTGC ATCCTTTGGT TACCCCATCA 
GCTATGGCTT CTATTCCTGC CCTTCCTCAA ACAGCAGGAC CTACTTCTAC ACCTTTATTG 
CCCCAAGTAC TTAGTATCCC ACCCTTGGTA CAACCTGTTG CCAGTGTGCC TGCTGTGCAG 
CAGACACTAA TTCATAGTCA GCCTCAACCA GCTTTACTTC CCAACCAGCC CCACACTCAC 
TGTCCTGAAA TAGATGCTGA CTTACAGCCC AAAGCTCCTG GAATTGATGA CATAAAGACT 
CTAGAGGAAA AGCTGCGGTC TCTCTTTAGT GAACCGAGCT CATCTGGAGC CCAACATACA 
TCGGTCTCAC TAGAGACTTC ACTGGTAGTA GAGACCACTG TCACGCCAGG TATCCCAACC 
ACTGCTATTG CACCAAGCAA GCTCATGACA TCTACTACAA GTACATGCTT ACCACCAACC 
AGTTTGCAAT TAGGAACATC TGGTTTGTCA GTTATACCAG TGGTCACGCC TGGGCAGGTT 
TCTACCCCAG TCAGCTATGT GTCAGCCCCA GGCAGCACTA CATCAGGAGT GAAAGCTGGA 
ACTGCTCCAT CAAAGCCACC TTTGACTAAA GCTCCAGTGC TGCCAGTGGG TACTGAACTT 
CCAGCTGGTA CTCCACCCAG CGAGCAGCTG CCACCTTTTC CAGGACCTTC ACTAACTCAG 
TCCCAGCAAC CTCTGGAAGA TCTTGATGCT CAGTTGAGAA GAACACTTAG TCCAGAGACT 
ATTGCGGTGA CATCTACAAT TGGTCCTGTG TCCATGGTGG CTCCAACAGC AGTCACAGAA 
GCAGGAGCAC AGCCTCAGAA GGATGTTTCT CAGATCACAG AAGGTCCTGT CCTAGCGACT 
AGTTCGGGAA CTGGTGTTTT TAAGATGGGA AGATTTCAGG TTTCAGTTGC AATGGATGAT 
GTTCAAAAGG AGGGTAAAAA TAAGTTAGAA GATGGAAAGT CTGTTCATTT TGAATCCAGC 
ACTTCAGAGT CCTCAGTGCT ATCAAGTAGT AGTCCAGAAA GTACCCTGGT GAAGACAGAG 
CCTAATGGGA TAACCATCCC TGGCATCTCA TCAGATATGC CAGATAGTGC CCACAAAACT 
CCTGCCTCAG AAGCAAAGTC AGAAACTGGG CAGCCTACCA AGGTTGGACG TTTTCAGGTG 
ACAACTACAG CAAACAAAGT AGGTCGTTTC TCTGTGTCAA GAACTGAAGA CAAGCTCATT 
GAGGCAAAGA AAGAGGGACC AATAGCGTCT CCTCCTTTTA TGGATTTGGA ACAAGGTGTT 
CTTCCAGCTG TGATACCAAA GAAAGAAAAG CCTGAACTGT CAGAGCCTTC ACATCTGAAC 
GGACCATCTT CTGACCTGGA GGCTGCCTTC CTGAGCAGGG ATGTGGATGA TGGTTCAGGT 
AGTCCACACT CCCCCCATCA GCTGAGCTCA AAGAGCCTGC CTATCCAGAA TCTAAGTCAA 
AGCCTTAGTA ATTCATTCAA CTCCTCTTAC ATGAGTAGTG ACAATGAGTC AGATATTGAA 
GATGAAGATT TAAAATTAGA GCTGCGAAGA CTACGAGAAA AACATCTTAA AGAGATTCAA 
GACCTGCAGA GTCGCCAGAA GCATGAAATT GAATCTTTGT ATACCAAACT GGGAAAGGTT 
CCCCCTGCTG TCATTATCCC CCCAGCTGCC CCCCTTTCAG GGAGAAGAAG GCGACCCACT 
AAAAGCAAAG GCAGCAAGTC TAGTCGCAGC AGTTCCTTGG GGAATAAAAG TCCCCAGCTT 
TCAGGTAACC TGTCTGGTCA GAGTGCTGCT TCAGTTTTGC ACGCTCAGCA GACCCTCCAC 
CCTCCTGGCA ACATCCCTGA GACTGGGCCA AATCAGCTGT TACAGCCCCT TAAGCCATCT 
CCCTCCAGTG ACAACCTCTA CTCAGCCTTT ACCAGTGATG GTGCCATTTC AGTCCCAAGC 
CTTTCTGCTC CAGGTCAAGG GACCAGCAGC ACAAACACTG TCGGGGGAAC AGTGAACAGC 
CAAGCTGCCC CAGCTCAGCC TCCTGCCATG ACATCCAGCA GGAAGGGCAC ATTCACAGAT 
GACCTACACA AGTTGGTAGA CAATTGGGCC CGAGATGCCA TGAATCTCTC AGGCAGAAGA 
GGAAGCAAAG GACACATGAA TTATGAGGGC CCTGGAATGG CAAGAAAGTT CTCCGCACCT 
GGTCAGCTGT GCATCTCCAT GACCTCAAAT CTGGGTGGTT CTGCCCCCAT CTCTGCAGCA 
TCAGCTACCT CTCTAGGTCA CTTCACCAAG TCTATGTGCC CCCCACAGCA GTACGGTTTT 
CCAGCTCCCC CCTTTGGCAC TCAGTGGAGT GGGACGGGTG GCCCGGCACC ACAGCCACTT 
GGCCAGTTCC AGCCTGTGGG AACTGCCTCC TTACAGAATT TCAACATCAG CAATTTGCAG 
AAATCCATCA GCAACCCCCC AGGTTCCAAC CTGCGGACCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963820.1
CDS	994..7338
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQSTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_014108345.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108345.2

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGCAG 
CAGGGAGTAC AGCAGACAGC CCCTCCACAA CAGACAGCGC AGTATTCGCT TCCACAGACA 
TCAGCCTCCA GTGAGGCCAC TATTGCACAA CCAGTCAGTC AGTCTCAGGC TCCACAGGTC 
TTGCCTCAAA TATCAGCTGG AAAACAGAGC ACTCAGGGAG TCTCTCAAGT TGCTCCTCCA 
GAGCCAGTTC CAGTGGCACA GGCACAGCCT ACCACTTTGG TCTCCTCTAT AGACAGTGCA 
CATTCAGATG TTGCTTCAGG AATGAGTGAT GGCAATGAGA ATGTCCCGTC CTCCAGTGGA 
AGGCATGAAG GGAGAGCTAC AAAACGACAT TACCGAAAAT CTGTAAGAAG TCGCTCTCGT 
CATGAAAAGA CTTCGCGTCC AAAATTAAGA ATTTTGAATG TTTCAAATAA AGGAGACCGA 
GTAGTAGAAT GTCAACTAGA GACTCATAAT CGGAAAATGG TTACATTCAA ATTTGATTTA 
GATGGTGACA ATCCTGAGGA GATAGCAACA ATTATGGTGA ACAATGACTT TATTCTAGCA 
ATGGAGAGAG AGTCCTTTGT GGATCAAGTA CGAGAAATTA TTGAAAAGGC TGATGAAATG 
CTCAGTGAGG ATGTCAGTGT GGAACCAGAG GGTGACCAGG GATTGGAGAG TCTGCAGGGA 
AAGGATGACT ATGGCTTTAC AGGTTCTCAA AAATTGGAAG GAGAATTCAA ACAACCAGTT 
CCTGCGTCTT CCATGCCACA GCAAATAGGC ATTCCTACCA GTTCTTTAAC TCAAGTTGTT 
CATTCTGCTG GAAGACGGTT TATAGTGAGT CCTGTGCCAG AAAGTCGATT ACGAGAATCA 
AAAGTTTTCA CTAGTGAAAT ACCAGATACA GTTGCTGCCT CTACTCACAG CGCTGGAATG 
AACTTGTCTC ACTCTGCTTC ATCCCTTAGC CTACAGCAAG CCTTTTCTGA ACTTAGACAT 
GTGCAGATGA CAGAAGGGCC CAGCACAGCA CCTCCCAACT TCAGTCATAC AGGACCAACA 
TTTCCAGTTG TTCCTCCTTC CATGAGTAGC ATTGCTGGAG TCCCAACCAC AGCAGTAGCC 
ACACCTTCAG TATCAGTCCC TGCAACTAGC AGCCCTCTAA ATGACAGTTC CACATCAGTA 
ATTCAATCTG AGATTCCAGT GCCCACTGAA ACAGGGGTTG GTGGAGTTCC CACCTGCACA 
AGTGTAATGC CTTCAAGTGG TCTCCCTGTA CCACCCATAT CTGAATCGCC AATATTTTCC 
ACTATGGTTT CAAGCATCAC AGTACCTACA GTTGTCTCAA TATCAGCAAC ATCCCAGCCA 
GTTCAAGCTC CCACATCTGG GAGCACTGTT TCTAGCATAG GAACTTTGCC TTCTATACCA 
GTTTCAACAG CTTTAGCCTC TGCAGTGGGC AGTGTTGCTA TCCCAGGTGC TAAGCCTCCA 
CCTGTACTAT CACAGCAGGC AGCAGGCAGT ACTACTGGGG CAGCCACATT AACATCCGTT 
CCTGCCATCA CTCCGTTCCC AAGCATAGCT TCACAGCCAT CTCTTCAGCT TAGCAGCAGC 
ACCTCTGCTC CAACTCTAGC TGAAACAGTG GTGGTTAGTG CACACTCACT AGATAAAACA 
TCTCATAGCA GTACAACTGG ATTGGCTTTG TCCCTGTCTG CATCATCTTC CTCCCCTGGA 
GCAGGATTAT CTAGTTCTAT TTCTCAGCCT GGTGGGGTGC ATCCTTTGGT TACCCCATCA 
GCTATGGCTT CTATTCCTGC CCTTCCTCAA ACAGCAGGAC CTACTTCTAC ACCTTTATTG 
CCCCAAGTAC TTAGTATCCC ACCCTTGGTA CAACCTGTTG CCAGTGTGCC TGCTGTGCAG 
CAGACACTAA TTCATAGTCA GCCTCAACCA GCTTTACTTC CCAACCAGCC CCACACTCAC 
TGTCCTGAAA TAGATGCTGA CTTACAGCCC AAAGCTCCTG GAATTGATGA CATAAAGACT 
CTAGAGGAAA AGCTGCGGTC TCTCTTTAGT GAACCGAGCT CATCTGGAGC CCAACATACA 
TCGGTCTCAC TAGAGACTTC ACTGGTAGTA GAGACCACTG TCACGCCAGG TATCCCAACC 
ACTGCTATTG CACCAAGCAA GCTCATGACA TCTACTACAA GTACATGCTT ACCACCAACC 
AGTTTGCAAT TAGGAACATC TGGTTTGTCA GTTATACCAG TGGTCACGCC TGGGCAGGTT 
TCTACCCCAG TCAGCTATGT GTCAGCCCCA GGCAGCACTA CATCAGGAGT GAAAGCTGGA 
ACTGCTCCAT CAAAGCCACC TTTGACTAAA GCTCCAGTGC TGCCAGTGGG TACTGAACTT 
CCAGCTGGTA CTCCACCCAG CGAGCAGCTG CCACCTTTTC CAGGACCTTC ACTAACTCAG 
TCCCAGCAAC CTCTGGAAGA TCTTGATGCT CAGTTGAGAA GAACACTTAG TCCAGAGACT 
ATTGCGGTGA CATCTACAAT TGGTCCTGTG TCCATGGTGG CTCCAACAGC AGTCACAGAA 
GCAGGAGCAC AGCCTCAGAA GGATGTTTCT CAGATCACAG AAGGTCCTGT CCTAGCGACT 
AGTTCGGGAA CTGGTGTTTT TAAGATGGGA AGATTTCAGG TTTCAGTTGC AATGGATGAT 
GTTCAAAAGG AGGGTAAAAA TAAGTTAGAA GATGGAAAGT CTGTTCATTT TGAATCCAGC 
ACTTCAGAGT CCTCAGTGCT ATCAAGTAGT AGTCCAGAAA GTACCCTGGT GAAGACAGAG 
CCTAATGGGA TAACCATCCC TGGCATCTCA TCAGATATGC CAGATAGTGC CCACAAAACT 
CCTGCCTCAG AAGCAAAGTC AGAAACTGGG CAGCCTACCA AGGTTGGACG TTTTCAGGTG 
ACAACTACAG CAAACAAAGT AGGTCGTTTC TCTGTGTCAA GAACTGAAGA CAAGCTCATT 
GAGGCAAAGA AAGAGGGACC AATAGCGTCT CCTCCTTTTA TGGATTTGGA ACAAGGTGTT 
CTTCCAGCTG TGATACCAAA GAAAGAAAAG CCTGAACTGT CAGAGCCTTC ACATCTGAAC 
GGACCATCTT CTGACCTGGA GGCTGCCTTC CTGAGCAGGG ATGTGGATGA TGGTTCAGGT 
AGTCCACACT CCCCCCATCA GCTGAGCTCA AAGAGCCTGC CTATCCAGAA TCTAAGTCAA 
AGCCTTAGTA ATTCATTCAA CTCCTCTTAC ATGAGTAGTG ACAATGAGTC AGATATTGAA 
GATGAAGATT TAAAATTAGA GCTGCGAAGA CTACGAGAAA AACATCTTAA AGAGATTCAA 
GACCTGCAGA GTCGCCAGAA GCATGAAATT GAATCTTTGT ATACCAAACT GGGAAAGGTT 
CCCCCTGCTG TCATTATCCC CCCAGCTGCC CCCCTTTCAG GGAGAAGAAG GCGACCCACT 
AAAAGCAAAG GCAGCAAGTC TAGTCGCAGC AGTTCCTTGG GGAATAAAAG TCCCCAGCTT 
TCAGGTAACC TGTCTGGTCA GAGTGCTGCT TCAGTTTTGC ACGCTCAGCA GACCCTCCAC 
CCTCCTGGCA ACATCCCTGA GACTGGGCCA AATCAGCTGT TACAGCCCCT TAAGCCATCT 
CCCTCCAGTG ACAACCTCTA CTCAGCCTTT ACCAGTGATG GTGCCATTTC AGTCCCAAGC 
CTTTCTGCTC CAGGTCAAGG GACCAGCAGC ACAAACACTG TCGGGGGAAC AGTGAACAGC 
CAAGCTGCCC CAGCTCAGCC TCCTGCCATG ACATCCAGCA GGAAGGGCAC ATTCACAGAT 
GACCTACACA AGTTGGTAGA CAATTGGGCC CGAGATGCCA TGAATCTCTC AGGCAGAAGA 
GGAAGCAAAG GACACATGAA TTATGAGGGC CCTGGAATGG CAAGAAAGTT CTCCGCACCT 
GGTCAGCTGT GCATCTCCAT GACCTCAAAT CTGGGTGGTT CTGCCCCCAT CTCTGCAGCA 
TCAGCTACCT CTCTAGGTCA CTTCACCAAG TCTATGTGCC CCCCACAGCA GTACGGTTTT 
CCAGCTCCCC CCTTTGGCAC TCAGTGGAGT GGGACGGGTG GCCCGGCACC ACAGCCACTT 
GGCCAGTTCC AGCCTGTGGG AACTGCCTCC TTACAGAATT TCAACATCAG CAATTTGCAG 
AAATCCATCA GCAACCCCCC AGGTTCCAAC CTGCGGACCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_014108345.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963820.1 Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X21, mRNA.

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CloneID	OCe43589
Clone ID Related Accession (Same CDS sequence)	XM_014108345.2 , XM_014108345.1
Accession Version	XM_014108345.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase WNK1 isoform X21
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGCAG 
CAGGGAGTAC AGCAGACAGC CCCTCCACAA CAGACAGCGC AGTATTCGCT TCCACAGACA 
TCAGCCTCCA GTGAGGCCAC TATTGCACAA CCAGTCAGTC AGTCTCAGGC TCCACAGGTC 
TTGCCTCAAA TATCAGCTGG AAAACAGAGC ACTCAGGGAG TCTCTCAAGT TGCTCCTCCA 
GAGCCAGTTC CAGTGGCACA GGCACAGCCT ACCACTTTGG TCTCCTCTAT AGACAGTGCA 
CATTCAGATG TTGCTTCAGG AATGAGTGAT GGCAATGAGA ATGTCCCGTC CTCCAGTGGA 
AGGCATGAAG GGAGAGCTAC AAAACGACAT TACCGAAAAT CTGTAAGAAG TCGCTCTCGT 
CATGAAAAGA CTTCGCGTCC AAAATTAAGA ATTTTGAATG TTTCAAATAA AGGAGACCGA 
GTAGTAGAAT GTCAACTAGA GACTCATAAT CGGAAAATGG TTACATTCAA ATTTGATTTA 
GATGGTGACA ATCCTGAGGA GATAGCAACA ATTATGGTGA ACAATGACTT TATTCTAGCA 
ATGGAGAGAG AGTCCTTTGT GGATCAAGTA CGAGAAATTA TTGAAAAGGC TGATGAAATG 
CTCAGTGAGG ATGTCAGTGT GGAACCAGAG GGTGACCAGG GATTGGAGAG TCTGCAGGGA 
AAGGATGACT ATGGCTTTAC AGGTTCTCAA AAATTGGAAG GAGAATTCAA ACAACCAGTT 
CCTGCGTCTT CCATGCCACA GCAAATAGGC ATTCCTACCA GTTCTTTAAC TCAAGTTGTT 
CATTCTGCTG GAAGACGGTT TATAGTGAGT CCTGTGCCAG AAAGTCGATT ACGAGAATCA 
AAAGTTTTCA CTAGTGAAAT ACCAGATACA GTTGCTGCCT CTACTCACAG CGCTGGAATG 
AACTTGTCTC ACTCTGCTTC ATCCCTTAGC CTACAGCAAG CCTTTTCTGA ACTTAGACAT 
GTGCAGATGA CAGAAGGGCC CAGCACAGCA CCTCCCAACT TCAGTCATAC AGGACCAACA 
TTTCCAGTTG TTCCTCCTTC CATGAGTAGC ATTGCTGGAG TCCCAACCAC AGCAGTAGCC 
ACACCTTCAG TATCAGTCCC TGCAACTAGC AGCCCTCTAA ATGACAGTTC CACATCAGTA 
ATTCAATCTG AGATTCCAGT GCCCACTGAA ACAGGGGTTG GTGGAGTTCC CACCTGCACA 
AGTGTAATGC CTTCAAGTGG TCTCCCTGTA CCACCCATAT CTGAATCGCC AATATTTTCC 
ACTATGGTTT CAAGCATCAC AGTACCTACA GTTGTCTCAA TATCAGCAAC ATCCCAGCCA 
GTTCAAGCTC CCACATCTGG GAGCACTGTT TCTAGCATAG GAACTTTGCC TTCTATACCA 
GTTTCAACAG CTTTAGCCTC TGCAGTGGGC AGTGTTGCTA TCCCAGGTGC TAAGCCTCCA 
CCTGTACTAT CACAGCAGGC AGCAGGCAGT ACTACTGGGG CAGCCACATT AACATCCGTT 
CCTGCCATCA CTCCGTTCCC AAGCATAGCT TCACAGCCAT CTCTTCAGCT TAGCAGCAGC 
ACCTCTGCTC CAACTCTAGC TGAAACAGTG GTGGTTAGTG CACACTCACT AGATAAAACA 
TCTCATAGCA GTACAACTGG ATTGGCTTTG TCCCTGTCTG CATCATCTTC CTCCCCTGGA 
GCAGGATTAT CTAGTTCTAT TTCTCAGCCT GGTGGGGTGC ATCCTTTGGT TACCCCATCA 
GCTATGGCTT CTATTCCTGC CCTTCCTCAA ACAGCAGGAC CTACTTCTAC ACCTTTATTG 
CCCCAAGTAC TTAGTATCCC ACCCTTGGTA CAACCTGTTG CCAGTGTGCC TGCTGTGCAG 
CAGACACTAA TTCATAGTCA GCCTCAACCA GCTTTACTTC CCAACCAGCC CCACACTCAC 
TGTCCTGAAA TAGATGCTGA CTTACAGCCC AAAGCTCCTG GAATTGATGA CATAAAGACT 
CTAGAGGAAA AGCTGCGGTC TCTCTTTAGT GAACCGAGCT CATCTGGAGC CCAACATACA 
TCGGTCTCAC TAGAGACTTC ACTGGTAGTA GAGACCACTG TCACGCCAGG TATCCCAACC 
ACTGCTATTG CACCAAGCAA GCTCATGACA TCTACTACAA GTACATGCTT ACCACCAACC 
AGTTTGCAAT TAGGAACATC TGGTTTGTCA GTTATACCAG TGGTCACGCC TGGGCAGGTT 
TCTACCCCAG TCAGCTATGT GTCAGCCCCA GGCAGCACTA CATCAGGAGT GAAAGCTGGA 
ACTGCTCCAT CAAAGCCACC TTTGACTAAA GCTCCAGTGC TGCCAGTGGG TACTGAACTT 
CCAGCTGGTA CTCCACCCAG CGAGCAGCTG CCACCTTTTC CAGGACCTTC ACTAACTCAG 
TCCCAGCAAC CTCTGGAAGA TCTTGATGCT CAGTTGAGAA GAACACTTAG TCCAGAGACT 
ATTGCGGTGA CATCTACAAT TGGTCCTGTG TCCATGGTGG CTCCAACAGC AGTCACAGAA 
GCAGGAGCAC AGCCTCAGAA GGATGTTTCT CAGATCACAG AAGGTCCTGT CCTAGCGACT 
AGTTCGGGAA CTGGTGTTTT TAAGATGGGA AGATTTCAGG TTTCAGTTGC AATGGATGAT 
GTTCAAAAGG AGGGTAAAAA TAAGTTAGAA GATGGAAAGT CTGTTCATTT TGAATCCAGC 
ACTTCAGAGT CCTCAGTGCT ATCAAGTAGT AGTCCAGAAA GTACCCTGGT GAAGACAGAG 
CCTAATGGGA TAACCATCCC TGGCATCTCA TCAGATATGC CAGATAGTGC CCACAAAACT 
CCTGCCTCAG AAGCAAAGTC AGAAACTGGG CAGCCTACCA AGGTTGGACG TTTTCAGGTG 
ACAACTACAG CAAACAAAGT AGGTCGTTTC TCTGTGTCAA GAACTGAAGA CAAGCTCATT 
GAGGCAAAGA AAGAGGGACC AATAGCGTCT CCTCCTTTTA TGGATTTGGA ACAAGGTGTT 
CTTCCAGCTG TGATACCAAA GAAAGAAAAG CCTGAACTGT CAGAGCCTTC ACATCTGAAC 
GGACCATCTT CTGACCTGGA GGCTGCCTTC CTGAGCAGGG ATGTGGATGA TGGTTCAGGT 
AGTCCACACT CCCCCCATCA GCTGAGCTCA AAGAGCCTGC CTATCCAGAA TCTAAGTCAA 
AGCCTTAGTA ATTCATTCAA CTCCTCTTAC ATGAGTAGTG ACAATGAGTC AGATATTGAA 
GATGAAGATT TAAAATTAGA GCTGCGAAGA CTACGAGAAA AACATCTTAA AGAGATTCAA 
GACCTGCAGA GTCGCCAGAA GCATGAAATT GAATCTTTGT ATACCAAACT GGGAAAGGTT 
CCCCCTGCTG TCATTATCCC CCCAGCTGCC CCCCTTTCAG GGAGAAGAAG GCGACCCACT 
AAAAGCAAAG GCAGCAAGTC TAGTCGCAGC AGTTCCTTGG GGAATAAAAG TCCCCAGCTT 
TCAGGTAACC TGTCTGGTCA GAGTGCTGCT TCAGTTTTGC ACGCTCAGCA GACCCTCCAC 
CCTCCTGGCA ACATCCCTGA GACTGGGCCA AATCAGCTGT TACAGCCCCT TAAGCCATCT 
CCCTCCAGTG ACAACCTCTA CTCAGCCTTT ACCAGTGATG GTGCCATTTC AGTCCCAAGC 
CTTTCTGCTC CAGGTCAAGG GACCAGCAGC ACAAACACTG TCGGGGGAAC AGTGAACAGC 
CAAGCTGCCC CAGCTCAGCC TCCTGCCATG ACATCCAGCA GGAAGGGCAC ATTCACAGAT 
GACCTACACA AGTTGGTAGA CAATTGGGCC CGAGATGCCA TGAATCTCTC AGGCAGAAGA 
GGAAGCAAAG GACACATGAA TTATGAGGGC CCTGGAATGG CAAGAAAGTT CTCCGCACCT 
GGTCAGCTGT GCATCTCCAT GACCTCAAAT CTGGGTGGTT CTGCCCCCAT CTCTGCAGCA 
TCAGCTACCT CTCTAGGTCA CTTCACCAAG TCTATGTGCC CCCCACAGCA GTACGGTTTT 
CCAGCTCCCC CCTTTGGCAC TCAGTGGAGT GGGACGGGTG GCCCGGCACC ACAGCCACTT 
GGCCAGTTCC AGCCTGTGGG AACTGCCTCC TTACAGAATT TCAACATCAG CAATTTGCAG 
AAATCCATCA GCAACCCCCC AGGTTCCAAC CTGCGGACCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963820.1
CDS	99..6443
Translation	MSGGAAEKQSSTPSSLFLSPPAPSPKNGSSSDSSVGEKLGGAAPDAGAGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPAVGVSVAAPQSAPPEPPREETLTAAAAVQVAQPPPAAAGPGEPVSSGPAAAAAAAAPSSAGRDRQASQPNHVGSKEEPSAARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEGSLKQQVEQQSSTSQAGVKQIPSVSTGVPTASTTSASVSTQVEPEEPEADQHQQLQYQQTSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTLPGHTASVVQAQSQPHGVYPPSSVQQGVQQTAPPQQTAQYSLPQTSASSEATIAQPVSQSQAPQVLPQISAGKQSTQGVSQVAPPEPVPVAQAQPTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRATKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAMERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFTGSQKLEGEFKQPVPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSEIPDTVAASTHSAGMNLSHSASSLSLQQAFSELRHVQMTEGPSTAPPNFSHTGPTFPVVPPSMSSIAGVPTTAVATPSVSVPATSSPLNDSSTSVIQSEIPVPTETGVGGVPTCTSVMPSSGLPVPPISESPIFSTMVSSITVPTVVSISATSQPVQAPTSGSTVSSIGTLPSIPVSTALASAVGSVAIPGAKPPPVLSQQAAGSTTGAATLTSVPAITPFPSIASQPSLQLSSSTSAPTLAETVVVSAHSLDKTSHSSTTGLALSLSASSSSPGAGLSSSISQPGGVHPLVTPSAMASIPALPQTAGPTSTPLLPQVLSIPPLVQPVASVPAVQQTLIHSQPQPALLPNQPHTHCPEIDADLQPKAPGIDDIKTLEEKLRSLFSEPSSSGAQHTSVSLETSLVVETTVTPGIPTTAIAPSKLMTSTTSTCLPPTSLQLGTSGLSVIPVVTPGQVSTPVSYVSAPGSTTSGVKAGTAPSKPPLTKAPVLPVGTELPAGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPETIAVTSTIGPVSMVAPTAVTEAGAQPQKDVSQITEGPVLATSSGTGVFKMGRFQVSVAMDDVQKEGKNKLEDGKSVHFESSTSESSVLSSSSPESTLVKTEPNGITIPGISSDMPDSAHKTPASEAKSETGQPTKVGRFQVTTTANKVGRFSVSRTEDKLIEAKKEGPIASPPFMDLEQGVLPAVIPKKEKPELSEPSHLNGPSSDLEAAFLSRDVDDGSGSPHSPHQLSSKSLPIQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHAQQTLHPPGNIPETGPNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGTVNSQAAPAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPAPPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_014108345.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris WNK lysine deficient protein kinase 1 (WNK1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108345.1

ORF Insert Sequence:

ATGTCTGGTG GCGCCGCAGA GAAGCAGAGC AGCACTCCGA GCTCCCTGTT CCTCTCGCCA 
CCGGCGCCCT CCCCCAAGAA TGGCTCCAGC TCCGACTCCT CGGTGGGGGA GAAACTGGGC 
GGCGCGGCGC CCGACGCTGG GGCCGGCCGC ACGGAGGAGT ACCGGCGTCG CCGCCACACC 
ATGGACAAGG ACAGCCGTGG CGCGGCGGCG ACCACCACCA CCACCGAGCA CCGCTTCTTC 
CGCCGCAGCG TCATCTGCGA CTCCAACGCC ACCGCGCTGG AGCTGCCCGG CCTCCCTCTG 
CCTCTGCCCC AGCCCGCGGT GGGGGTGTCG GTGGCTGCGC CGCAGAGCGC CCCGCCGGAG 
CCGCCGCGTG AGGAGACCTT GACGGCCGCC GCCGCCGTGC AGGTAGCCCA GCCGCCCCCC 
GCCGCTGCCG GCCCCGGGGA GCCAGTCAGC TCAGGCCCTG CCGCTGCCGC TGCCGCCGCC 
GCCCCCAGCA GTGCCGGCAG AGATCGGCAG GCGTCCCAGC CCAACCATGT GGGGAGCAAA 
GAGGAGCCTT CGGCGGCCAG GAGCGGCAGC GGCGGCGGCA GCGCCAAGGA ACCACAGGAG 
GAACGCAGCC AGCAGCAGGA TGATATCGAA GAGCTGGAGA CCAAGGCCGT GGGAATGTCC 
AACGACGGCC GCTTTCTCAA GTTCGACATC GAAATTGGCA GAGGCTCCTT TAAGACCGTC 
TACAAAGGTC TGGACACTGA AACCACGGTG GAGGTCGCCT GGTGTGAACT GCAGGATCGC 
AAATTAACAA AGTCTGAGAG GCAGAGATTT AAAGAAGAGG CTGAAATGTT AAAGGGCCTT 
CAGCATCCCA ATATTGTTCG ATTCTATGAT TCCTGGGAGT CCACAGTAAA AGGAAAGAAA 
TGCATTGTTT TGGTGACGGA ACTTATGACA TCTGGAACAC TTAAAACGTA CCTGAAAAGG 
TTTAAGGTGA TGAAGATCAA AGTTCTAAGA AGCTGGTGCC GTCAGATCCT TAAAGGACTT 
CAGTTTCTTC ATACTCGAAC TCCACCTATT ATTCACCGAG ATCTTAAGTG TGACAATATC 
TTTATCACTG GCCCTACAGG CTCAGTCAAG ATTGGAGACC TAGGTCTGGC AACCCTGAAG 
CGGGCTTCTT TTGCCAAGAG TGTGATAGGT ACCCCAGAGT TCATGGCCCC TGAGATGTAT 
GAGGAAAAAT ATGATGAATC TGTTGACGTT TACGCTTTTG GAATGTGCAT GCTTGAGATG 
GCCACATCTG AATACCCTTA CTCAGAGTGC CAAAATGCTG CGCAGATCTA CCGTCGAGTG 
ACCAGTGGAG TAAAGCCAGC CAGTTTTGAC AAAGTAGCAA TTCCTGAAGT GAAGGAAATT 
ATTGAAGGAT GCATAAGGCA AAACAAAGAT GAAAGGTATT CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGGGTA CGGGTAGAAT TAGCAGAAGA AGATGATGGA 
GAAAAGATAG CCATTAAGTT ATGGCTACGT ATTGAAGATA TTAAGAAATT AAAGGGAAAA 
TACAAAGACA ATGAAGCTAT TGAGTTTTCC TTTGACTTGG AAAGAGATGT GCCGGAAGAT 
GTTGCTCAAG AAATGGTAGA GTCTGGGTAC GTCTGTGAAG GTGATCACAA GACCATGGCA 
AAAGCCATCA AAGATAGAGT ATCATTAATT AAGAGAAAAC GAGAACAGCG GCAGTTGGTT 
CGAGAGGAGC AAGAAAAAAG AAAGCAGGAA GAGGGCAGTC TCAAACAGCA GGTAGAACAA 
CAATCAAGTA CTTCCCAGGC AGGAGTCAAG CAGATCCCTT CTGTCAGCAC TGGCGTGCCT 
ACTGCTTCTA CCACTTCAGC TTCAGTTTCT ACGCAAGTAG AACCTGAAGA ACCTGAGGCA 
GATCAACATC AACAGCTACA GTACCAACAA ACTAGTATAT CTGTATTATC TGACGGGACT 
GTTGACAGTG GTCAAGGATC TTCTGTCTTC ACAGAATCTC GAGTGAGCAG CCAACAGACA 
GTTTCATATG GGTCCCAGCA TGAACAGGCA CATTCTACTG GCACACTCCC AGGGCATACA 
GCTTCTGTTG TCCAGGCACA ATCTCAGCCC CATGGGGTCT ATCCACCCTC AAGTGTGCAG 
CAGGGAGTAC AGCAGACAGC CCCTCCACAA CAGACAGCGC AGTATTCGCT TCCACAGACA 
TCAGCCTCCA GTGAGGCCAC TATTGCACAA CCAGTCAGTC AGTCTCAGGC TCCACAGGTC 
TTGCCTCAAA TATCAGCTGG AAAACAGAGC ACTCAGGGAG TCTCTCAAGT TGCTCCTCCA 
GAGCCAGTTC CAGTGGCACA GGCACAGCCT ACCACTTTGG TCTCCTCTAT AGACAGTGCA 
CATTCAGATG TTGCTTCAGG AATGAGTGAT GGCAATGAGA ATGTCCCGTC CTCCAGTGGA 
AGGCATGAAG GGAGAGCTAC AAAACGACAT TACCGAAAAT CTGTAAGAAG TCGCTCTCGT 
CATGAAAAGA CTTCGCGTCC AAAATTAAGA ATTTTGAATG TTTCAAATAA AGGAGACCGA 
GTAGTAGAAT GTCAACTAGA GACTCATAAT CGGAAAATGG TTACATTCAA ATTTGATTTA 
GATGGTGACA ATCCTGAGGA GATAGCAACA ATTATGGTGA ACAATGACTT TATTCTAGCA 
ATGGAGAGAG AGTCCTTTGT GGATCAAGTA CGAGAAATTA TTGAAAAGGC TGATGAAATG 
CTCAGTGAGG ATGTCAGTGT GGAACCAGAG GGTGACCAGG GATTGGAGAG TCTGCAGGGA 
AAGGATGACT ATGGCTTTAC AGGTTCTCAA AAATTGGAAG GAGAATTCAA ACAACCAGTT 
CCTGCGTCTT CCATGCCACA GCAAATAGGC ATTCCTACCA GTTCTTTAAC TCAAGTTGTT 
CATTCTGCTG GAAGACGGTT TATAGTGAGT CCTGTGCCAG AAAGTCGATT ACGAGAATCA 
AAAGTTTTCA CTAGTGAAAT ACCAGATACA GTTGCTGCCT CTACTCACAG CGCTGGAATG 
AACTTGTCTC ACTCTGCTTC ATCCCTTAGC CTACAGCAAG CCTTTTCTGA ACTTAGACAT 
GTGCAGATGA CAGAAGGGCC CAGCACAGCA CCTCCCAACT TCAGTCATAC AGGACCAACA 
TTTCCAGTTG TTCCTCCTTC CATGAGTAGC ATTGCTGGAG TCCCAACCAC AGCAGTAGCC 
ACACCTTCAG TATCAGTCCC TGCAACTAGC AGCCCTCTAA ATGACAGTTC CACATCAGTA 
ATTCAATCTG AGATTCCAGT GCCCACTGAA ACAGGGGTTG GTGGAGTTCC CACCTGCACA 
AGTGTAATGC CTTCAAGTGG TCTCCCTGTA CCACCCATAT CTGAATCGCC AATATTTTCC 
ACTATGGTTT CAAGCATCAC AGTACCTACA GTTGTCTCAA TATCAGCAAC ATCCCAGCCA 
GTTCAAGCTC CCACATCTGG GAGCACTGTT TCTAGCATAG GAACTTTGCC TTCTATACCA 
GTTTCAACAG CTTTAGCCTC TGCAGTGGGC AGTGTTGCTA TCCCAGGTGC TAAGCCTCCA 
CCTGTACTAT CACAGCAGGC AGCAGGCAGT ACTACTGGGG CAGCCACATT AACATCCGTT 
CCTGCCATCA CTCCGTTCCC AAGCATAGCT TCACAGCCAT CTCTTCAGCT TAGCAGCAGC 
ACCTCTGCTC CAACTCTAGC TGAAACAGTG GTGGTTAGTG CACACTCACT AGATAAAACA 
TCTCATAGCA GTACAACTGG ATTGGCTTTG TCCCTGTCTG CATCATCTTC CTCCCCTGGA 
GCAGGATTAT CTAGTTCTAT TTCTCAGCCT GGTGGGGTGC ATCCTTTGGT TACCCCATCA 
GCTATGGCTT CTATTCCTGC CCTTCCTCAA ACAGCAGGAC CTACTTCTAC ACCTTTATTG 
CCCCAAGTAC TTAGTATCCC ACCCTTGGTA CAACCTGTTG CCAGTGTGCC TGCTGTGCAG 
CAGACACTAA TTCATAGTCA GCCTCAACCA GCTTTACTTC CCAACCAGCC CCACACTCAC 
TGTCCTGAAA TAGATGCTGA CTTACAGCCC AAAGCTCCTG GAATTGATGA CATAAAGACT 
CTAGAGGAAA AGCTGCGGTC TCTCTTTAGT GAACCGAGCT CATCTGGAGC CCAACATACA 
TCGGTCTCAC TAGAGACTTC ACTGGTAGTA GAGACCACTG TCACGCCAGG TATCCCAACC 
ACTGCTATTG CACCAAGCAA GCTCATGACA TCTACTACAA GTACATGCTT ACCACCAACC 
AGTTTGCAAT TAGGAACATC TGGTTTGTCA GTTATACCAG TGGTCACGCC TGGGCAGGTT 
TCTACCCCAG TCAGCTATGT GTCAGCCCCA GGCAGCACTA CATCAGGAGT GAAAGCTGGA 
ACTGCTCCAT CAAAGCCACC TTTGACTAAA GCTCCAGTGC TGCCAGTGGG TACTGAACTT 
CCAGCTGGTA CTCCACCCAG CGAGCAGCTG CCACCTTTTC CAGGACCTTC ACTAACTCAG 
TCCCAGCAAC CTCTGGAAGA TCTTGATGCT CAGTTGAGAA GAACACTTAG TCCAGAGACT 
ATTGCGGTGA CATCTACAAT TGGTCCTGTG TCCATGGTGG CTCCAACAGC AGTCACAGAA 
GCAGGAGCAC AGCCTCAGAA GGATGTTTCT CAGATCACAG AAGGTCCTGT CCTAGCGACT 
AGTTCGGGAA CTGGTGTTTT TAAGATGGGA AGATTTCAGG TTTCAGTTGC AATGGATGAT 
GTTCAAAAGG AGGGTAAAAA TAAGTTAGAA GATGGAAAGT CTGTTCATTT TGAATCCAGC 
ACTTCAGAGT CCTCAGTGCT ATCAAGTAGT AGTCCAGAAA GTACCCTGGT GAAGACAGAG 
CCTAATGGGA TAACCATCCC TGGCATCTCA TCAGATATGC CAGATAGTGC CCACAAAACT 
CCTGCCTCAG AAGCAAAGTC AGAAACTGGG CAGCCTACCA AGGTTGGACG TTTTCAGGTG 
ACAACTACAG CAAACAAAGT AGGTCGTTTC TCTGTGTCAA GAACTGAAGA CAAGCTCATT 
GAGGCAAAGA AAGAGGGACC AATAGCGTCT CCTCCTTTTA TGGATTTGGA ACAAGGTGTT 
CTTCCAGCTG TGATACCAAA GAAAGAAAAG CCTGAACTGT CAGAGCCTTC ACATCTGAAC 
GGACCATCTT CTGACCTGGA GGCTGCCTTC CTGAGCAGGG ATGTGGATGA TGGTTCAGGT 
AGTCCACACT CCCCCCATCA GCTGAGCTCA AAGAGCCTGC CTATCCAGAA TCTAAGTCAA 
AGCCTTAGTA ATTCATTCAA CTCCTCTTAC ATGAGTAGTG ACAATGAGTC AGATATTGAA 
GATGAAGATT TAAAATTAGA GCTGCGAAGA CTACGAGAAA AACATCTTAA AGAGATTCAA 
GACCTGCAGA GTCGCCAGAA GCATGAAATT GAATCTTTGT ATACCAAACT GGGAAAGGTT 
CCCCCTGCTG TCATTATCCC CCCAGCTGCC CCCCTTTCAG GGAGAAGAAG GCGACCCACT 
AAAAGCAAAG GCAGCAAGTC TAGTCGCAGC AGTTCCTTGG GGAATAAAAG TCCCCAGCTT 
TCAGGTAACC TGTCTGGTCA GAGTGCTGCT TCAGTTTTGC ACGCTCAGCA GACCCTCCAC 
CCTCCTGGCA ACATCCCTGA GACTGGGCCA AATCAGCTGT TACAGCCCCT TAAGCCATCT 
CCCTCCAGTG ACAACCTCTA CTCAGCCTTT ACCAGTGATG GTGCCATTTC AGTCCCAAGC 
CTTTCTGCTC CAGGTCAAGG GACCAGCAGC ACAAACACTG TCGGGGGAAC AGTGAACAGC 
CAAGCTGCCC CAGCTCAGCC TCCTGCCATG ACATCCAGCA GGAAGGGCAC ATTCACAGAT 
GACCTACACA AGTTGGTAGA CAATTGGGCC CGAGATGCCA TGAATCTCTC AGGCAGAAGA 
GGAAGCAAAG GACACATGAA TTATGAGGGC CCTGGAATGG CAAGAAAGTT CTCCGCACCT 
GGTCAGCTGT GCATCTCCAT GACCTCAAAT CTGGGTGGTT CTGCCCCCAT CTCTGCAGCA 
TCAGCTACCT CTCTAGGTCA CTTCACCAAG TCTATGTGCC CCCCACAGCA GTACGGTTTT 
CCAGCTCCCC CCTTTGGCAC TCAGTGGAGT GGGACGGGTG GCCCGGCACC ACAGCCACTT 
GGCCAGTTCC AGCCTGTGGG AACTGCCTCC TTACAGAATT TCAACATCAG CAATTTGCAG 
AAATCCATCA GCAACCCCCC AGGTTCCAAC CTGCGGACCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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WNK1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information: