LOC477699 cDNA ORF clone, Canis lupus familiaris(dog)

The following LOC477699 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC477699 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe27347 XM_534893.6
Latest version!
Canis lupus familiaris alpha-2-macroglobulin (LOC477699), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
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OCe27347 XM_534893.5 Canis lupus familiaris alpha-2-macroglobulin (LOC477699), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe27347
    Clone ID Related Accession (Same CDS sequence) XM_534893.5 , XM_534893.6
    Accession Version XM_534893.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4428bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product alpha-2-macroglobulin
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_534893.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAGGAAAA ACAAACTCCT TCATCCCAAT TTTATTCTCC TCCTTCTGGT CTTCCTGCCT 
    GCAGATGCCT CAGTCTCTGG AAAACCGCAG TACATGGTCC TAGTCCCCTC CCTGCTCCAC
    TCTGAAATCC CTGAGAAGGG CTGCCTCATT CTGAGCTACC TGAATGAGAC GGTGACTGTA
    AGTGCTACCT TGGAGTCCGT CAGGGAGAAT AGGAGCCTCT TCACTGACCT GGTGGGGGAA
    AAGGACTTAT TCAGCTGTAT CTCCTTCACT GTGCCAAGAT CTCCATCCAA TGAAGAGGTC
    ATGTTCCTCA CTATCCAAGT GAAAGGACCA ACACAAGAAT TCAAGAGACG GACCACAGTG
    GTGGTTAAAA ACCAAGAGAG TCTGGTCTTT GTCCAGACAG ACAAACCCAT CTACAAACCA
    GACCAGACAG TGAAGTTTCG TGTTGTCTCA TTGGATGAAA ATTTTCACCC CCTAAATGAG
    TTGATTCCAC TAGTATATAT TCAGGACCCA AAAGGAAATC GCATCACACA ATGGCAGAAA
    CTCAGATTAG AGAATGGTCT CAAGCAATTG AGCTTTCCCC TCTCATCTGA GCCCTTTCAG
    GGCTCCTATA AGGTGGTGGT GCAGAAGGAA TCAGGTGAAA GGACAGAGCA CCCCTTCACC
    GTGGAAGAAT TTGTGCTTCC CAAGTTTGAA GTGAAAGTAA CAATGCCAAG GATAATCACT
    ATCTTGGAAG AAGAAGTGAA TGTTTCAGTG TGTGGCCTAT ACACATATGG GAAGCCCGTC
    CCAGGACATA TAACTATGAG AGTGTGCAGA AAGTACAGTA ACCCTTCTAA CTGCTATAGT
    GGAGAGTCAC AGGCTGTCTG TGAGAAATTC AGTCATCAGC TAAACAGCCA GGGCTGCTTC
    TCTCAGCAAA TAAAAACCAA GATCTTCCAG ATGAAAAGAC AAGGATATGA AATGAAACTT
    GAAGTGGAGG CCAAGATCCA AGAAGAAGGA ACAGAGGTAG AATTAACTGG AAAAGGATCC
    ACTGAAATTA CAAGAACCAT AACCAAACTC TCATTCGTGA AAGTGGACCC ATATTTTAGA
    CAAGGGATCC CCTTCTTTGG GCAGGTGCAA CTCATGGATG GGAAAGGTGT CCCCATGCCA
    AATGAACTCA TCTTCATCAC AGCAAATGAA GCTAATTATA ATTCCAATGC TACCACTGAT
    GAGCGTGGCC TGGTTCAGTT CTCTATCAAT ACCACCAACA TTATGGGTAC CTCTCTCACT
    GTCAGGGTCA GACACAAGGA TTCTCGTTCC TGTTATGGTT ACCAGTGGCT GTCAATAGAA
    CACAAAGAAG CTGATCACAC TGCTAATCTT GTATTCTCCC TAAGCAAGAG TTTTGTCCAC
    CTAGAGCCCA TGCCTCGTGA ACTGCCCTGT GGCCAAACTC AGACAATCCA AGCACATTAT
    ATTCTGAATG GACAGGTCCT GCAGGAGCTG AAGGAGCTCG TGTTCTATTA TCTGATAATG
    GCAAAGGGAG GCATTGTCCG GAAAGGGACA CATGTGCTGC CTGTGGAACA GGGAGAAATG
    AAAGGCCATT TTTCCATGTC AGTCCCCGTG GAGTCAGACA TTGCTCCCGT TGCTCGATTA
    CTCATCTATG CCATTTTACC TGATGGGGAA GTAGTTGGGG ATTCTGCAAA ATATAAGGTT
    GAAAACTGTC TAGCCAACAA GGTGGACTTG AGCTTCCGGC CAACACAAAG TCTCCCGGCC
    TCACAAGCCC ACCTGCAAAT TTCAGCATCT CCTCAGTCCC TGTGCGCGCT CCGAGCTGTG
    GACCAAAGTG TGCTGCTTGC TAAGCCAGAA GCTGAGCTGT CCGCTGCCTC GGTTTATAAC
    CTGCTACCAG TGAAGGACCT CAGCGGTTTC CCTGGGAGCT TAAATCAACA GGAGGAAGAT
    GATAGAGACT GTGTCCATCA CCATAATATC TATATCAATG GAGTCAAGTA TTCCCCTGTA
    TCAAATACAA ATGAAAAAGA TATGTATAGC TTCCTAAAGG ATATGGGCTT AAAGGTATTC
    ACCAACACAA AGATTCATAA ACCCAAAGTA TGTGAACAGC CTGAACACAT GGTAGCGCAC
    TCATCTCGAA GACTATTTGC TTCTCCAGTA GTGTCTGAAT TGCAAAGATC CCATGAGCTT
    ACAGACATGG TCCATTTTTC CGAGCCTCTC ACCGAAACTG TGCGAAAGTA CTTCCCTGAG
    ACGTGGATCT GGGATTTGGT GGTGGTAGAC TCATCAGGGG TGGCTGAAGT GGAAGTGACA
    GTCCCTGATA CCATCACAGA GTGGAAGGCA GGGGCGCTCT GTCTGTCCAA CGACACTGGA
    CTTGGTCTCT CTCTTCCCAC CTCCCTCCAA GCCTTCCAGC CCTTCTTTGT GGAGCTCACA
    ATGCCCTACT CTGTGATCCG TGGAGAAGCC TTCAACCTCA AGGCCACGGT CCTCAACTAC
    CTTTCCAAAT GCATCCGGGT TAGTGTGCAG CTAGACCCAA ATCCTGCATT CCTAGTACAG
    GAGAAGGAAC AAGAGTCTCA TTGCATCTGT GGGAATGGCC GGCAAACCGT GTCCTGGGCA
    GTAACGCCAA AGGCATTAGG AAATGTGAAT TTCACCGTGA GTGCAGAGGC ACTGGAGTCG
    CAAGAGCTAT GTGGGACTGA GGTGACAGTG GTCCCTGAAT ATGGAAAGAA AGACACCATC
    ATTAAATCCC TGCTGGTTGA ACCTGAAGGA CTAGAGACGG AAACAACATT CAACTCCCTG
    CTCTGTGCAT CAGATGCTGA GGTGTCTGAA CAATTATCTT TAAAGTTGCC AAAGAATGTA
    GTAGAAGAAT CTGCTCGAGC CTCCATCTCA GTATTGGGAG ACATACTGGG ATCTGCCATG
    CAAAACATAC AGAATCTCCT CCGAATGCCT TATGGCTGTG GAGAACAGAA CATGGTCCTC
    TTTGCTCCCA ACATCTATGT CTTGAATTAT CTAAATAAAA CACACCAGCT GACTCCAGAA
    GTCATGTCCA AGGCCATTGG TTACCTCAAT ACTGGTTACC AGAGACAGCT GAAGTACAAA
    CACCGTGATG GCTCCTATAG CACCTTTGGA GAGCAATATG GCAAGAATAA GGGCAATACT
    TGGCTCACTG CCTTTGTACT GAAGACTTTT GCCCAAGCTC GAACCCATAT CTTCATTGAT
    GAAGCACACA TCACCCAAGC CCTCATGTGG CTCTCCCAAA AGCAGAAGGA CAATGGCTGT
    TTCAGGAGTT CCGGGTCTCT GCTCAACAAT GCCATTAAGG GTGGAGTGGA AGACGAAGTG
    ACCCTCTCTG CCTACGTCAC CATTGCCCTT CTGGAGATCC CTCTCTCAAC CACTCACCCT
    GTTGTCCGCA GTGCTCTGTT CTGCCTGGAG TCAGCCTGGA AGTCAGCAAA GGAAGGACCC
    CATGGAAAGC ATGTCTACAC CAAGGCACTG TTGGCCTACG CTTTTGCCCT GGCAGGTAAC
    CAGGAAAAGA GGAGAGAGAT ACTCACCTTA CTTAATGAGG AAGCTGTGAA GGAAGAGAGT
    TCCGTCCACT GGGAGCGACC TCAGAAACCC AGGGCACCAG TGGAGCGTTT TTACCAACCC
    CGGGCTCCCT CAGCTGAGGT GGAGATGACG TCCTATGTGC TCCTGGCTTA CCTCGTGGCC
    CAGCCTGCCC CGACCTCAGA GGAGCTGACT TCTGCCTCTC GCATCGTGAA GTGGATCACA
    AAGCAACAGA ATTCCCAGGG GGGCTTCTCC TCCACCCAGG ACACAGTGGT GGCTCTCCAG
    TCCTTGTCCA GATACGGAGC AGCCACTTTC ACCAGGACTG GGAAGCCAAC CCAGGTGACC
    ATCCAATATT CAGGGACATT TTTCACAAAA TTCCAAGTGG ACGATGACAA CCGCCTGTTA
    CTACAGCAGA CCTCATTGCC AAAGGTGCCT GAGGAATACA CCATGACGGT GACAGGAGGA
    GGGTGTGTCT ACCTCCAGAC ATCCTTGAAG TACAATATTC TTCCAGAAAG GGAAGAGTCC
    CCATTCGCTT TGGAGGTACA GACTCTGCCC CAAACTTGTG ACAGACCCAA AGCCCACACC
    AGCTTCCATA TCTTGCTGAA TGTCAGTTAC ACCGGGAGTC GTCCTGAATC CAATATGGTG
    ATTACTGACG TGAAGATGGT ATCTGGCTTC ATTCCCCTGA AACCAACAGT GAAAATGCTT
    GAGAGATCTA GCCATGTGAG CCGAACAGAA GTCAGCAACA ACCATGTGTT GATTTATCTG
    GATAAGGTGA CAAATCAGAC ACTCAGCTTG TCCTTCATGG TTTTGCAAGA TGTCCCAGTA
    AGAGATCTGA AGCCAGCCAT AGTGAAAGTC TATGATTACT ATGAGACAGA TGAGTTTGCA
    ATTGCTGAGT ACAATGCTCC TTGCAGCAAG GATCATGGAA ATGCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_534893.3
    CDS123..4550
    Translation

    Target ORF information:

    RefSeq Version XM_534893.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris alpha-2-macroglobulin (LOC477699), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_534893.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAGGAAAA ACAAACTCCT TCATCCCAAT TTTATTCTCC TCCTTCTGGT CTTCCTGCCT 
    GCAGATGCCT CAGTCTCTGG AAAACCGCAG TACATGGTCC TAGTCCCCTC CCTGCTCCAC
    TCTGAAATCC CTGAGAAGGG CTGCCTCATT CTGAGCTACC TGAATGAGAC GGTGACTGTA
    AGTGCTACCT TGGAGTCCGT CAGGGAGAAT AGGAGCCTCT TCACTGACCT GGTGGGGGAA
    AAGGACTTAT TCAGCTGTAT CTCCTTCACT GTGCCAAGAT CTCCATCCAA TGAAGAGGTC
    ATGTTCCTCA CTATCCAAGT GAAAGGACCA ACACAAGAAT TCAAGAGACG GACCACAGTG
    GTGGTTAAAA ACCAAGAGAG TCTGGTCTTT GTCCAGACAG ACAAACCCAT CTACAAACCA
    GACCAGACAG TGAAGTTTCG TGTTGTCTCA TTGGATGAAA ATTTTCACCC CCTAAATGAG
    TTGATTCCAC TAGTATATAT TCAGGACCCA AAAGGAAATC GCATCACACA ATGGCAGAAA
    CTCAGATTAG AGAATGGTCT CAAGCAATTG AGCTTTCCCC TCTCATCTGA GCCCTTTCAG
    GGCTCCTATA AGGTGGTGGT GCAGAAGGAA TCAGGTGAAA GGACAGAGCA CCCCTTCACC
    GTGGAAGAAT TTGTGCTTCC CAAGTTTGAA GTGAAAGTAA CAATGCCAAG GATAATCACT
    ATCTTGGAAG AAGAAGTGAA TGTTTCAGTG TGTGGCCTAT ACACATATGG GAAGCCCGTC
    CCAGGACATA TAACTATGAG AGTGTGCAGA AAGTACAGTA ACCCTTCTAA CTGCTATAGT
    GGAGAGTCAC AGGCTGTCTG TGAGAAATTC AGTCATCAGC TAAACAGCCA GGGCTGCTTC
    TCTCAGCAAA TAAAAACCAA GATCTTCCAG ATGAAAAGAC AAGGATATGA AATGAAACTT
    GAAGTGGAGG CCAAGATCCA AGAAGAAGGA ACAGAGGTAG AATTAACTGG AAAAGGATCC
    ACTGAAATTA CAAGAACCAT AACCAAACTC TCATTCGTGA AAGTGGACCC ATATTTTAGA
    CAAGGGATCC CCTTCTTTGG GCAGGTGCAA CTCATGGATG GGAAAGGTGT CCCCATGCCA
    AATGAACTCA TCTTCATCAC AGCAAATGAA GCTAATTATA ATTCCAATGC TACCACTGAT
    GAGCGTGGCC TGGTTCAGTT CTCTATCAAT ACCACCAACA TTATGGGTAC CTCTCTCACT
    GTCAGGGTCA GACACAAGGA TTCTCGTTCC TGTTATGGTT ACCAGTGGCT GTCAATAGAA
    CACAAAGAAG CTGATCACAC TGCTAATCTT GTATTCTCCC TAAGCAAGAG TTTTGTCCAC
    CTAGAGCCCA TGCCTCGTGA ACTGCCCTGT GGCCAAACTC AGACAATCCA AGCACATTAT
    ATTCTGAATG GACAGGTCCT GCAGGAGCTG AAGGAGCTCG TGTTCTATTA TCTGATAATG
    GCAAAGGGAG GCATTGTCCG GAAAGGGACA CATGTGCTGC CTGTGGAACA GGGAGAAATG
    AAAGGCCATT TTTCCATGTC AGTCCCCGTG GAGTCAGACA TTGCTCCCGT TGCTCGATTA
    CTCATCTATG CCATTTTACC TGATGGGGAA GTAGTTGGGG ATTCTGCAAA ATATAAGGTT
    GAAAACTGTC TAGCCAACAA GGTGGACTTG AGCTTCCGGC CAACACAAAG TCTCCCGGCC
    TCACAAGCCC ACCTGCAAAT TTCAGCATCT CCTCAGTCCC TGTGCGCGCT CCGAGCTGTG
    GACCAAAGTG TGCTGCTTGC TAAGCCAGAA GCTGAGCTGT CCGCTGCCTC GGTTTATAAC
    CTGCTACCAG TGAAGGACCT CAGCGGTTTC CCTGGGAGCT TAAATCAACA GGAGGAAGAT
    GATAGAGACT GTGTCCATCA CCATAATATC TATATCAATG GAGTCAAGTA TTCCCCTGTA
    TCAAATACAA ATGAAAAAGA TATGTATAGC TTCCTAAAGG ATATGGGCTT AAAGGTATTC
    ACCAACACAA AGATTCATAA ACCCAAAGTA TGTGAACAGC CTGAACACAT GGTAGCGCAC
    TCATCTCGAA GACTATTTGC TTCTCCAGTA GTGTCTGAAT TGCAAAGATC CCATGAGCTT
    ACAGACATGG TCCATTTTTC CGAGCCTCTC ACCGAAACTG TGCGAAAGTA CTTCCCTGAG
    ACGTGGATCT GGGATTTGGT GGTGGTAGAC TCATCAGGGG TGGCTGAAGT GGAAGTGACA
    GTCCCTGATA CCATCACAGA GTGGAAGGCA GGGGCGCTCT GTCTGTCCAA CGACACTGGA
    CTTGGTCTCT CTCTTCCCAC CTCCCTCCAA GCCTTCCAGC CCTTCTTTGT GGAGCTCACA
    ATGCCCTACT CTGTGATCCG TGGAGAAGCC TTCAACCTCA AGGCCACGGT CCTCAACTAC
    CTTTCCAAAT GCATCCGGGT TAGTGTGCAG CTAGACCCAA ATCCTGCATT CCTAGTACAG
    GAGAAGGAAC AAGAGTCTCA TTGCATCTGT GGGAATGGCC GGCAAACCGT GTCCTGGGCA
    GTAACGCCAA AGGCATTAGG AAATGTGAAT TTCACCGTGA GTGCAGAGGC ACTGGAGTCG
    CAAGAGCTAT GTGGGACTGA GGTGACAGTG GTCCCTGAAT ATGGAAAGAA AGACACCATC
    ATTAAATCCC TGCTGGTTGA ACCTGAAGGA CTAGAGACGG AAACAACATT CAACTCCCTG
    CTCTGTGCAT CAGATGCTGA GGTGTCTGAA CAATTATCTT TAAAGTTGCC AAAGAATGTA
    GTAGAAGAAT CTGCTCGAGC CTCCATCTCA GTATTGGGAG ACATACTGGG ATCTGCCATG
    CAAAACATAC AGAATCTCCT CCGAATGCCT TATGGCTGTG GAGAACAGAA CATGGTCCTC
    TTTGCTCCCA ACATCTATGT CTTGAATTAT CTAAATAAAA CACACCAGCT GACTCCAGAA
    GTCATGTCCA AGGCCATTGG TTACCTCAAT ACTGGTTACC AGAGACAGCT GAAGTACAAA
    CACCGTGATG GCTCCTATAG CACCTTTGGA GAGCAATATG GCAAGAATAA GGGCAATACT
    TGGCTCACTG CCTTTGTACT GAAGACTTTT GCCCAAGCTC GAACCCATAT CTTCATTGAT
    GAAGCACACA TCACCCAAGC CCTCATGTGG CTCTCCCAAA AGCAGAAGGA CAATGGCTGT
    TTCAGGAGTT CCGGGTCTCT GCTCAACAAT GCCATTAAGG GTGGAGTGGA AGACGAAGTG
    ACCCTCTCTG CCTACGTCAC CATTGCCCTT CTGGAGATCC CTCTCTCAAC CACTCACCCT
    GTTGTCCGCA GTGCTCTGTT CTGCCTGGAG TCAGCCTGGA AGTCAGCAAA GGAAGGACCC
    CATGGAAAGC ATGTCTACAC CAAGGCACTG TTGGCCTACG CTTTTGCCCT GGCAGGTAAC
    CAGGAAAAGA GGAGAGAGAT ACTCACCTTA CTTAATGAGG AAGCTGTGAA GGAAGAGAGT
    TCCGTCCACT GGGAGCGACC TCAGAAACCC AGGGCACCAG TGGAGCGTTT TTACCAACCC
    CGGGCTCCCT CAGCTGAGGT GGAGATGACG TCCTATGTGC TCCTGGCTTA CCTCGTGGCC
    CAGCCTGCCC CGACCTCAGA GGAGCTGACT TCTGCCTCTC GCATCGTGAA GTGGATCACA
    AAGCAACAGA ATTCCCAGGG GGGCTTCTCC TCCACCCAGG ACACAGTGGT GGCTCTCCAG
    TCCTTGTCCA GATACGGAGC AGCCACTTTC ACCAGGACTG GGAAGCCAAC CCAGGTGACC
    ATCCAATATT CAGGGACATT TTTCACAAAA TTCCAAGTGG ACGATGACAA CCGCCTGTTA
    CTACAGCAGA CCTCATTGCC AAAGGTGCCT GAGGAATACA CCATGACGGT GACAGGAGGA
    GGGTGTGTCT ACCTCCAGAC ATCCTTGAAG TACAATATTC TTCCAGAAAG GGAAGAGTCC
    CCATTCGCTT TGGAGGTACA GACTCTGCCC CAAACTTGTG ACAGACCCAA AGCCCACACC
    AGCTTCCATA TCTTGCTGAA TGTCAGTTAC ACCGGGAGTC GTCCTGAATC CAATATGGTG
    ATTACTGACG TGAAGATGGT ATCTGGCTTC ATTCCCCTGA AACCAACAGT GAAAATGCTT
    GAGAGATCTA GCCATGTGAG CCGAACAGAA GTCAGCAACA ACCATGTGTT GATTTATCTG
    GATAAGGTGA CAAATCAGAC ACTCAGCTTG TCCTTCATGG TTTTGCAAGA TGTCCCAGTA
    AGAGATCTGA AGCCAGCCAT AGTGAAAGTC TATGATTACT ATGAGACAGA TGAGTTTGCA
    ATTGCTGAGT ACAATGCTCC TTGCAGCAAG GATCATGGAA ATGCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27347
    Clone ID Related Accession (Same CDS sequence) XM_534893.5 , XM_534893.6
    Accession Version XM_534893.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4428bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product alpha-2-macroglobulin
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_534893.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAGGAAAA ACAAACTCCT TCATCCCAAT TTTATTCTCC TCCTTCTGGT CTTCCTGCCT 
    GCAGATGCCT CAGTCTCTGG AAAACCGCAG TACATGGTCC TAGTCCCCTC CCTGCTCCAC
    TCTGAAATCC CTGAGAAGGG CTGCCTCATT CTGAGCTACC TGAATGAGAC GGTGACTGTA
    AGTGCTACCT TGGAGTCCGT CAGGGAGAAT AGGAGCCTCT TCACTGACCT GGTGGGGGAA
    AAGGACTTAT TCAGCTGTAT CTCCTTCACT GTGCCAAGAT CTCCATCCAA TGAAGAGGTC
    ATGTTCCTCA CTATCCAAGT GAAAGGACCA ACACAAGAAT TCAAGAGACG GACCACAGTG
    GTGGTTAAAA ACCAAGAGAG TCTGGTCTTT GTCCAGACAG ACAAACCCAT CTACAAACCA
    GACCAGACAG TGAAGTTTCG TGTTGTCTCA TTGGATGAAA ATTTTCACCC CCTAAATGAG
    TTGATTCCAC TAGTATATAT TCAGGACCCA AAAGGAAATC GCATCACACA ATGGCAGAAA
    CTCAGATTAG AGAATGGTCT CAAGCAATTG AGCTTTCCCC TCTCATCTGA GCCCTTTCAG
    GGCTCCTATA AGGTGGTGGT GCAGAAGGAA TCAGGTGAAA GGACAGAGCA CCCCTTCACC
    GTGGAAGAAT TTGTGCTTCC CAAGTTTGAA GTGAAAGTAA CAATGCCAAG GATAATCACT
    ATCTTGGAAG AAGAAGTGAA TGTTTCAGTG TGTGGCCTAT ACACATATGG GAAGCCCGTC
    CCAGGACATA TAACTATGAG AGTGTGCAGA AAGTACAGTA ACCCTTCTAA CTGCTATAGT
    GGAGAGTCAC AGGCTGTCTG TGAGAAATTC AGTCATCAGC TAAACAGCCA GGGCTGCTTC
    TCTCAGCAAA TAAAAACCAA GATCTTCCAG ATGAAAAGAC AAGGATATGA AATGAAACTT
    GAAGTGGAGG CCAAGATCCA AGAAGAAGGA ACAGAGGTAG AATTAACTGG AAAAGGATCC
    ACTGAAATTA CAAGAACCAT AACCAAACTC TCATTCGTGA AAGTGGACCC ATATTTTAGA
    CAAGGGATCC CCTTCTTTGG GCAGGTGCAA CTCATGGATG GGAAAGGTGT CCCCATGCCA
    AATGAACTCA TCTTCATCAC AGCAAATGAA GCTAATTATA ATTCCAATGC TACCACTGAT
    GAGCGTGGCC TGGTTCAGTT CTCTATCAAT ACCACCAACA TTATGGGTAC CTCTCTCACT
    GTCAGGGTCA GACACAAGGA TTCTCGTTCC TGTTATGGTT ACCAGTGGCT GTCAATAGAA
    CACAAAGAAG CTGATCACAC TGCTAATCTT GTATTCTCCC TAAGCAAGAG TTTTGTCCAC
    CTAGAGCCCA TGCCTCGTGA ACTGCCCTGT GGCCAAACTC AGACAATCCA AGCACATTAT
    ATTCTGAATG GACAGGTCCT GCAGGAGCTG AAGGAGCTCG TGTTCTATTA TCTGATAATG
    GCAAAGGGAG GCATTGTCCG GAAAGGGACA CATGTGCTGC CTGTGGAACA GGGAGAAATG
    AAAGGCCATT TTTCCATGTC AGTCCCCGTG GAGTCAGACA TTGCTCCCGT TGCTCGATTA
    CTCATCTATG CCATTTTACC TGATGGGGAA GTAGTTGGGG ATTCTGCAAA ATATAAGGTT
    GAAAACTGTC TAGCCAACAA GGTGGACTTG AGCTTCCGGC CAACACAAAG TCTCCCGGCC
    TCACAAGCCC ACCTGCAAAT TTCAGCATCT CCTCAGTCCC TGTGCGCGCT CCGAGCTGTG
    GACCAAAGTG TGCTGCTTGC TAAGCCAGAA GCTGAGCTGT CCGCTGCCTC GGTTTATAAC
    CTGCTACCAG TGAAGGACCT CAGCGGTTTC CCTGGGAGCT TAAATCAACA GGAGGAAGAT
    GATAGAGACT GTGTCCATCA CCATAATATC TATATCAATG GAGTCAAGTA TTCCCCTGTA
    TCAAATACAA ATGAAAAAGA TATGTATAGC TTCCTAAAGG ATATGGGCTT AAAGGTATTC
    ACCAACACAA AGATTCATAA ACCCAAAGTA TGTGAACAGC CTGAACACAT GGTAGCGCAC
    TCATCTCGAA GACTATTTGC TTCTCCAGTA GTGTCTGAAT TGCAAAGATC CCATGAGCTT
    ACAGACATGG TCCATTTTTC CGAGCCTCTC ACCGAAACTG TGCGAAAGTA CTTCCCTGAG
    ACGTGGATCT GGGATTTGGT GGTGGTAGAC TCATCAGGGG TGGCTGAAGT GGAAGTGACA
    GTCCCTGATA CCATCACAGA GTGGAAGGCA GGGGCGCTCT GTCTGTCCAA CGACACTGGA
    CTTGGTCTCT CTCTTCCCAC CTCCCTCCAA GCCTTCCAGC CCTTCTTTGT GGAGCTCACA
    ATGCCCTACT CTGTGATCCG TGGAGAAGCC TTCAACCTCA AGGCCACGGT CCTCAACTAC
    CTTTCCAAAT GCATCCGGGT TAGTGTGCAG CTAGACCCAA ATCCTGCATT CCTAGTACAG
    GAGAAGGAAC AAGAGTCTCA TTGCATCTGT GGGAATGGCC GGCAAACCGT GTCCTGGGCA
    GTAACGCCAA AGGCATTAGG AAATGTGAAT TTCACCGTGA GTGCAGAGGC ACTGGAGTCG
    CAAGAGCTAT GTGGGACTGA GGTGACAGTG GTCCCTGAAT ATGGAAAGAA AGACACCATC
    ATTAAATCCC TGCTGGTTGA ACCTGAAGGA CTAGAGACGG AAACAACATT CAACTCCCTG
    CTCTGTGCAT CAGATGCTGA GGTGTCTGAA CAATTATCTT TAAAGTTGCC AAAGAATGTA
    GTAGAAGAAT CTGCTCGAGC CTCCATCTCA GTATTGGGAG ACATACTGGG ATCTGCCATG
    CAAAACATAC AGAATCTCCT CCGAATGCCT TATGGCTGTG GAGAACAGAA CATGGTCCTC
    TTTGCTCCCA ACATCTATGT CTTGAATTAT CTAAATAAAA CACACCAGCT GACTCCAGAA
    GTCATGTCCA AGGCCATTGG TTACCTCAAT ACTGGTTACC AGAGACAGCT GAAGTACAAA
    CACCGTGATG GCTCCTATAG CACCTTTGGA GAGCAATATG GCAAGAATAA GGGCAATACT
    TGGCTCACTG CCTTTGTACT GAAGACTTTT GCCCAAGCTC GAACCCATAT CTTCATTGAT
    GAAGCACACA TCACCCAAGC CCTCATGTGG CTCTCCCAAA AGCAGAAGGA CAATGGCTGT
    TTCAGGAGTT CCGGGTCTCT GCTCAACAAT GCCATTAAGG GTGGAGTGGA AGACGAAGTG
    ACCCTCTCTG CCTACGTCAC CATTGCCCTT CTGGAGATCC CTCTCTCAAC CACTCACCCT
    GTTGTCCGCA GTGCTCTGTT CTGCCTGGAG TCAGCCTGGA AGTCAGCAAA GGAAGGACCC
    CATGGAAAGC ATGTCTACAC CAAGGCACTG TTGGCCTACG CTTTTGCCCT GGCAGGTAAC
    CAGGAAAAGA GGAGAGAGAT ACTCACCTTA CTTAATGAGG AAGCTGTGAA GGAAGAGAGT
    TCCGTCCACT GGGAGCGACC TCAGAAACCC AGGGCACCAG TGGAGCGTTT TTACCAACCC
    CGGGCTCCCT CAGCTGAGGT GGAGATGACG TCCTATGTGC TCCTGGCTTA CCTCGTGGCC
    CAGCCTGCCC CGACCTCAGA GGAGCTGACT TCTGCCTCTC GCATCGTGAA GTGGATCACA
    AAGCAACAGA ATTCCCAGGG GGGCTTCTCC TCCACCCAGG ACACAGTGGT GGCTCTCCAG
    TCCTTGTCCA GATACGGAGC AGCCACTTTC ACCAGGACTG GGAAGCCAAC CCAGGTGACC
    ATCCAATATT CAGGGACATT TTTCACAAAA TTCCAAGTGG ACGATGACAA CCGCCTGTTA
    CTACAGCAGA CCTCATTGCC AAAGGTGCCT GAGGAATACA CCATGACGGT GACAGGAGGA
    GGGTGTGTCT ACCTCCAGAC ATCCTTGAAG TACAATATTC TTCCAGAAAG GGAAGAGTCC
    CCATTCGCTT TGGAGGTACA GACTCTGCCC CAAACTTGTG ACAGACCCAA AGCCCACACC
    AGCTTCCATA TCTTGCTGAA TGTCAGTTAC ACCGGGAGTC GTCCTGAATC CAATATGGTG
    ATTACTGACG TGAAGATGGT ATCTGGCTTC ATTCCCCTGA AACCAACAGT GAAAATGCTT
    GAGAGATCTA GCCATGTGAG CCGAACAGAA GTCAGCAACA ACCATGTGTT GATTTATCTG
    GATAAGGTGA CAAATCAGAC ACTCAGCTTG TCCTTCATGG TTTTGCAAGA TGTCCCAGTA
    AGAGATCTGA AGCCAGCCAT AGTGAAAGTC TATGATTACT ATGAGACAGA TGAGTTTGCA
    ATTGCTGAGT ACAATGCTCC TTGCAGCAAG GATCATGGAA ATGCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_534893.3
    CDS114..4541
    Translation

    Target ORF information:

    RefSeq Version XM_534893.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris alpha-2-macroglobulin (LOC477699), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_534893.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAGGAAAA ACAAACTCCT TCATCCCAAT TTTATTCTCC TCCTTCTGGT CTTCCTGCCT 
    GCAGATGCCT CAGTCTCTGG AAAACCGCAG TACATGGTCC TAGTCCCCTC CCTGCTCCAC
    TCTGAAATCC CTGAGAAGGG CTGCCTCATT CTGAGCTACC TGAATGAGAC GGTGACTGTA
    AGTGCTACCT TGGAGTCCGT CAGGGAGAAT AGGAGCCTCT TCACTGACCT GGTGGGGGAA
    AAGGACTTAT TCAGCTGTAT CTCCTTCACT GTGCCAAGAT CTCCATCCAA TGAAGAGGTC
    ATGTTCCTCA CTATCCAAGT GAAAGGACCA ACACAAGAAT TCAAGAGACG GACCACAGTG
    GTGGTTAAAA ACCAAGAGAG TCTGGTCTTT GTCCAGACAG ACAAACCCAT CTACAAACCA
    GACCAGACAG TGAAGTTTCG TGTTGTCTCA TTGGATGAAA ATTTTCACCC CCTAAATGAG
    TTGATTCCAC TAGTATATAT TCAGGACCCA AAAGGAAATC GCATCACACA ATGGCAGAAA
    CTCAGATTAG AGAATGGTCT CAAGCAATTG AGCTTTCCCC TCTCATCTGA GCCCTTTCAG
    GGCTCCTATA AGGTGGTGGT GCAGAAGGAA TCAGGTGAAA GGACAGAGCA CCCCTTCACC
    GTGGAAGAAT TTGTGCTTCC CAAGTTTGAA GTGAAAGTAA CAATGCCAAG GATAATCACT
    ATCTTGGAAG AAGAAGTGAA TGTTTCAGTG TGTGGCCTAT ACACATATGG GAAGCCCGTC
    CCAGGACATA TAACTATGAG AGTGTGCAGA AAGTACAGTA ACCCTTCTAA CTGCTATAGT
    GGAGAGTCAC AGGCTGTCTG TGAGAAATTC AGTCATCAGC TAAACAGCCA GGGCTGCTTC
    TCTCAGCAAA TAAAAACCAA GATCTTCCAG ATGAAAAGAC AAGGATATGA AATGAAACTT
    GAAGTGGAGG CCAAGATCCA AGAAGAAGGA ACAGAGGTAG AATTAACTGG AAAAGGATCC
    ACTGAAATTA CAAGAACCAT AACCAAACTC TCATTCGTGA AAGTGGACCC ATATTTTAGA
    CAAGGGATCC CCTTCTTTGG GCAGGTGCAA CTCATGGATG GGAAAGGTGT CCCCATGCCA
    AATGAACTCA TCTTCATCAC AGCAAATGAA GCTAATTATA ATTCCAATGC TACCACTGAT
    GAGCGTGGCC TGGTTCAGTT CTCTATCAAT ACCACCAACA TTATGGGTAC CTCTCTCACT
    GTCAGGGTCA GACACAAGGA TTCTCGTTCC TGTTATGGTT ACCAGTGGCT GTCAATAGAA
    CACAAAGAAG CTGATCACAC TGCTAATCTT GTATTCTCCC TAAGCAAGAG TTTTGTCCAC
    CTAGAGCCCA TGCCTCGTGA ACTGCCCTGT GGCCAAACTC AGACAATCCA AGCACATTAT
    ATTCTGAATG GACAGGTCCT GCAGGAGCTG AAGGAGCTCG TGTTCTATTA TCTGATAATG
    GCAAAGGGAG GCATTGTCCG GAAAGGGACA CATGTGCTGC CTGTGGAACA GGGAGAAATG
    AAAGGCCATT TTTCCATGTC AGTCCCCGTG GAGTCAGACA TTGCTCCCGT TGCTCGATTA
    CTCATCTATG CCATTTTACC TGATGGGGAA GTAGTTGGGG ATTCTGCAAA ATATAAGGTT
    GAAAACTGTC TAGCCAACAA GGTGGACTTG AGCTTCCGGC CAACACAAAG TCTCCCGGCC
    TCACAAGCCC ACCTGCAAAT TTCAGCATCT CCTCAGTCCC TGTGCGCGCT CCGAGCTGTG
    GACCAAAGTG TGCTGCTTGC TAAGCCAGAA GCTGAGCTGT CCGCTGCCTC GGTTTATAAC
    CTGCTACCAG TGAAGGACCT CAGCGGTTTC CCTGGGAGCT TAAATCAACA GGAGGAAGAT
    GATAGAGACT GTGTCCATCA CCATAATATC TATATCAATG GAGTCAAGTA TTCCCCTGTA
    TCAAATACAA ATGAAAAAGA TATGTATAGC TTCCTAAAGG ATATGGGCTT AAAGGTATTC
    ACCAACACAA AGATTCATAA ACCCAAAGTA TGTGAACAGC CTGAACACAT GGTAGCGCAC
    TCATCTCGAA GACTATTTGC TTCTCCAGTA GTGTCTGAAT TGCAAAGATC CCATGAGCTT
    ACAGACATGG TCCATTTTTC CGAGCCTCTC ACCGAAACTG TGCGAAAGTA CTTCCCTGAG
    ACGTGGATCT GGGATTTGGT GGTGGTAGAC TCATCAGGGG TGGCTGAAGT GGAAGTGACA
    GTCCCTGATA CCATCACAGA GTGGAAGGCA GGGGCGCTCT GTCTGTCCAA CGACACTGGA
    CTTGGTCTCT CTCTTCCCAC CTCCCTCCAA GCCTTCCAGC CCTTCTTTGT GGAGCTCACA
    ATGCCCTACT CTGTGATCCG TGGAGAAGCC TTCAACCTCA AGGCCACGGT CCTCAACTAC
    CTTTCCAAAT GCATCCGGGT TAGTGTGCAG CTAGACCCAA ATCCTGCATT CCTAGTACAG
    GAGAAGGAAC AAGAGTCTCA TTGCATCTGT GGGAATGGCC GGCAAACCGT GTCCTGGGCA
    GTAACGCCAA AGGCATTAGG AAATGTGAAT TTCACCGTGA GTGCAGAGGC ACTGGAGTCG
    CAAGAGCTAT GTGGGACTGA GGTGACAGTG GTCCCTGAAT ATGGAAAGAA AGACACCATC
    ATTAAATCCC TGCTGGTTGA ACCTGAAGGA CTAGAGACGG AAACAACATT CAACTCCCTG
    CTCTGTGCAT CAGATGCTGA GGTGTCTGAA CAATTATCTT TAAAGTTGCC AAAGAATGTA
    GTAGAAGAAT CTGCTCGAGC CTCCATCTCA GTATTGGGAG ACATACTGGG ATCTGCCATG
    CAAAACATAC AGAATCTCCT CCGAATGCCT TATGGCTGTG GAGAACAGAA CATGGTCCTC
    TTTGCTCCCA ACATCTATGT CTTGAATTAT CTAAATAAAA CACACCAGCT GACTCCAGAA
    GTCATGTCCA AGGCCATTGG TTACCTCAAT ACTGGTTACC AGAGACAGCT GAAGTACAAA
    CACCGTGATG GCTCCTATAG CACCTTTGGA GAGCAATATG GCAAGAATAA GGGCAATACT
    TGGCTCACTG CCTTTGTACT GAAGACTTTT GCCCAAGCTC GAACCCATAT CTTCATTGAT
    GAAGCACACA TCACCCAAGC CCTCATGTGG CTCTCCCAAA AGCAGAAGGA CAATGGCTGT
    TTCAGGAGTT CCGGGTCTCT GCTCAACAAT GCCATTAAGG GTGGAGTGGA AGACGAAGTG
    ACCCTCTCTG CCTACGTCAC CATTGCCCTT CTGGAGATCC CTCTCTCAAC CACTCACCCT
    GTTGTCCGCA GTGCTCTGTT CTGCCTGGAG TCAGCCTGGA AGTCAGCAAA GGAAGGACCC
    CATGGAAAGC ATGTCTACAC CAAGGCACTG TTGGCCTACG CTTTTGCCCT GGCAGGTAAC
    CAGGAAAAGA GGAGAGAGAT ACTCACCTTA CTTAATGAGG AAGCTGTGAA GGAAGAGAGT
    TCCGTCCACT GGGAGCGACC TCAGAAACCC AGGGCACCAG TGGAGCGTTT TTACCAACCC
    CGGGCTCCCT CAGCTGAGGT GGAGATGACG TCCTATGTGC TCCTGGCTTA CCTCGTGGCC
    CAGCCTGCCC CGACCTCAGA GGAGCTGACT TCTGCCTCTC GCATCGTGAA GTGGATCACA
    AAGCAACAGA ATTCCCAGGG GGGCTTCTCC TCCACCCAGG ACACAGTGGT GGCTCTCCAG
    TCCTTGTCCA GATACGGAGC AGCCACTTTC ACCAGGACTG GGAAGCCAAC CCAGGTGACC
    ATCCAATATT CAGGGACATT TTTCACAAAA TTCCAAGTGG ACGATGACAA CCGCCTGTTA
    CTACAGCAGA CCTCATTGCC AAAGGTGCCT GAGGAATACA CCATGACGGT GACAGGAGGA
    GGGTGTGTCT ACCTCCAGAC ATCCTTGAAG TACAATATTC TTCCAGAAAG GGAAGAGTCC
    CCATTCGCTT TGGAGGTACA GACTCTGCCC CAAACTTGTG ACAGACCCAA AGCCCACACC
    AGCTTCCATA TCTTGCTGAA TGTCAGTTAC ACCGGGAGTC GTCCTGAATC CAATATGGTG
    ATTACTGACG TGAAGATGGT ATCTGGCTTC ATTCCCCTGA AACCAACAGT GAAAATGCTT
    GAGAGATCTA GCCATGTGAG CCGAACAGAA GTCAGCAACA ACCATGTGTT GATTTATCTG
    GATAAGGTGA CAAATCAGAC ACTCAGCTTG TCCTTCATGG TTTTGCAAGA TGTCCCAGTA
    AGAGATCTGA AGCCAGCCAT AGTGAAAGTC TATGATTACT ATGAGACAGA TGAGTTTGCA
    ATTGCTGAGT ACAATGCTCC TTGCAGCAAG GATCATGGAA ATGCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.