NWD1 gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_014121994.1	XP_013977469.1	NACHT domain- and WD repeat-containing protein 1 isoform X3
XM_014121993.1	XP_013977468.1	NACHT domain- and WD repeat-containing protein 1 isoform X2
XM_022406921.1	XP_022262629.1	NACHT domain- and WD repeat-containing protein 1 isoform X5
XM_022406922.1	XP_022262630.1	NACHT domain- and WD repeat-containing protein 1 isoform X6
XM_533883.5	XP_533883.5	NACHT domain- and WD repeat-containing protein 1 isoform X1
XM_022406916.1	XP_022262624.1	NACHT domain- and WD repeat-containing protein 1 isoform X1
XM_022406919.1	XP_022262627.1	NACHT domain- and WD repeat-containing protein 1 isoform X4
XM_022406917.1	XP_022262625.1	NACHT domain- and WD repeat-containing protein 1 isoform X2
XM_022406920.1	XP_022262628.1	NACHT domain- and WD repeat-containing protein 1 isoform X5
XM_022406918.1	XP_022262626.1	NACHT domain- and WD repeat-containing protein 1 isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe295550	XM_022406918.1 Latest version!	Canis lupus familiaris NACHT and WD repeat domain containing 1 (NWD1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCe295551	XM_022406919.1 Latest version!	Canis lupus familiaris NACHT and WD repeat domain containing 1 (NWD1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCe295552	XM_022406922.1 Latest version!	Canis lupus familiaris NACHT and WD repeat domain containing 1 (NWD1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCe41722	XM_014121994.1 Latest version!	Canis lupus familiaris NACHT and WD repeat domain containing 1 (NWD1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCe41722	XM_022406921.1 Latest version!	Canis lupus familiaris NACHT and WD repeat domain containing 1 (NWD1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCe41722	XM_022406920.1 Latest version!	Canis lupus familiaris NACHT and WD repeat domain containing 1 (NWD1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCe295548	XM_022406916.1 Latest version!	Canis lupus familiaris NACHT and WD repeat domain containing 1 (NWD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe295549	XM_022406917.1 Latest version!	Canis lupus familiaris NACHT and WD repeat domain containing 1 (NWD1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe41720	XM_533883.5 Latest version!	Canis lupus familiaris NACHT and WD repeat domain containing 1 (NWD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe41721	XM_014121993.1 Latest version!	Canis lupus familiaris NACHT and WD repeat domain containing 1 (NWD1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00

NWD1 ( XM_022406918.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022262626.1 Canis lupus familiaris NACHT and WD repeat domain containing 1 (NWD1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe295550
Clone ID Related Accession (Same CDS sequence)	XM_022406918.1
Accession Version	XM_022406918.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	NACHT domain- and WD repeat-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGG GCCCCCTCAG CCCGGGCACC CGCCAGCGAC TTCTCCAGGG TCAGGCAGAC 
CCCCTCCCAG CGCCTCTGTC CAGAAGTGTG GCCATCTTCA TCAGCTCCAC GGTCTCGGAT 
ATGGATGCAG AGAGAGAAGC TCTGCAGAGC ACTGCCTTCC CCGAAGTGCA GACCTTCTGC 
CAAAAGAATG GCCTGATGTT CGAGGTTGTT GACCTGAGGT GGGGTATTCG GAACACTGAG 
GCCACTGACC ACATGACCAC AGAACTCTGC TTGGAGGAGC TCGACCGGTG TCGGAAAACA 
TCCATAGGGC CGGCTTTTGT TGCCCTCTTA GGTGACCAGT ACGGCCCCTG TCCAGTCCCT 
ACACTGATCG AGGAGAAGGA GTGGGAGGCC CTGAGATCCC AGCTGGCCTC CAAGCCACGT 
GACCTGGAGC TGGTGGCTCG ACGCTTCCGG AGGGATGAGA ACGCCGTGCC CCCCACCTAC 
CTGCTTCAGG CCCTAGGCAC TGCGGAGACC CAGGGGCCCG AAGAGGCCAC CCTGACCTCT 
GTGCTGCGCT CCGGAGCCCA GGAGGCCCGG AGGCTGGGCC TCATCACCCA GGAGCAGTGG 
CACCGCTACC ACTGGTCAGT CATTGAGTGG GAGATAGACC GGGGCCTGCT GAGCTTGACA 
GACGGAGACC AGGGGGCGAC TGTCTTCTTG AGAGAGATCC AAGACCTCAA CAAACACATC 
CTGGAAGACT GCGCCCTCAA GATGGTGGAC CGGCTCGCAG ATGGCTGCCT GGACACAGAT 
GCCCAGAACC TTCTCAGTGG CCTCAAGGGA CGCATTGCCG ACACACAACC TGGAGTCCTC 
AAGGCCCACC ACCTGCCATG GAGCCGAGAC CTGGTGAACC CCAAAAACAA GGCTCATGCT 
CGGTACCTGA AAGAGCTGGG GGAGCAGTTT GTGGCCAGGG CTAACCACCA GGTCCTTGAG 
CACCTCCGGG AGCAGGAGGC AGGCAGGCAG GAGTTGGCGT GGCTCTACCA GGAGATCCGC 
CACCACCTGG GGCTGAGCAC CGAGGCCACC TGGACCTTTT GTGGCCGCCA GGAGCTCCTG 
GCCCAGCTGG GGCAGCGGCT CCGGCAGAGC GATGGCCATC CGCACTCACC CCTGGTGCTT 
TTTGGACCTC CAGGCATCGG AAAGACAGCG CTGATGTGCA AGTTGGCAGA ACAAATGCCG 
GGACTGCTTG GCCGCAAGAC AGTGACCGTC CTGCGGCTTC TGGGGATGTC ACAGATGAGC 
TCCGACGCCC GAGGCCTGCT CCAGAGCATC TGCTTCCAGG TGTGCCTGGC CTACGGGCTG 
CCCCTGCCCC CTGCCCAGGT CCTGGAGGCC CATGCCAGGG TGGTCCAGTT TTTCCACACC 
CTCCTCCACA CTGTCTCCTG CCGAAACTTC GAGTCACTTG TGATCCTGTT GGACTCCATG 
GATGATGTGG ATTCCATCTG CCGCGCCCGG AGGGTCCCCT GGCTGCCTCC CCGGTGCCCC 
CCGAGGATCC ACCTTATCCT CTCAGCCTGC TCGGGGAAGC CGGGGATTCT AGACATCATG 
AGACAGACGC TGACGGACCC AGAGGCCTAC TGGGAGGTGA AACCCCTCTC CGGAAACCAA 
GGGCAGGAGA TGATCCAGCT CCTGCTGGCC GCCTCGCGGA GGACGCTGAG CCCAGGGCAG 
AGGGAGCTGC TCTGGGCCAG CCTCCCAGAG TGTGGGCACC CAGGACGGCT GAGGCTGGCC 
TTTGAGGAGG CCCGGAAGTG GGCCTCCTTC ACCATACCTG CCCCTCTGGC CTCCACTGCT 
AAGGAAGCGA TCCATCAGCT GTGTGCCCGC CTGGAGCAGA CACACGGGCA GCTCCTGGTG 
GCCCACGTGC TGAGCTACAT CGTGTCCTCC CGGCATGGGC TCTCAGAGGC AGAGCTGAAG 
GACGTCCTGT CCTTGGACGA CGAGGTCCTG GAGGCGGTGT ACCAGGACTG GACCCCGCCC 
AGCAAGGAGC TGTTGCGCTT CCCCCCGTTG CTGTGGGTGA GGCTCCGTCG GGATCTCGGC 
CACTGCTTGG CCCGGCGGCC GGTGGACGGC TTCACGCTCC TGGCCATCGC CCACAGGCAG 
CTGACTGAGG TGATCCAAGA GCGCTACCTG TCTGGGCCCG AGAGAGCCAA GAGGCACGGA 
GTCTTGGCTG ACTTCTTCTC AGGGGCCTGG AGCCAGGGCA CAAAGAAACT CATCACCCTG 
CCACTCGTGG GGAAGCCCCT GAACCTGGAC CGCAAGGTGG CGCCTCAGCC CCTGTGGTTC 
TCAGACACAG TTGCAAATCT GCGGAAGCTG AAGGAGTTGC CCCATCACCT GGTCCACTCA 
GGCCGCTTCG AAGAGCTGAA GCAGGAGGTT CTGGGCAGCA TGAACTGGAT TTCCTGCCGG 
GGCGTCTCTG GGGGCATCGA GGGGCTGTTG GAGGACTTTG ACCTGTATGG CCCTCACCTG 
GATTGTCCCG AGGTCGGCCT GGTCCGGGAC GCCCTCCAGC TGTGCCGCCC GGCCCTGGAG 
CTCCGAGGCA TGGAGAGGAG CATCCTCTAC ACTGAACTCC TGGCCAGACT CCATTTCTTC 
GCCACCTCAC ATCCAGCGCT GGTGGGACAG CTGTGCCAAC AGGCCCAGAG CTGGTTCCGG 
CTGTGCCCCC ACCCTGTGCT GGTACCTCTC GGGGGGTTCC TCCAGCCCCC CGGGGGACCC 
CTCCGGGCAA CCTTCACTGG CTGTCACAAG GGCATCACTG CCATGGGCTG GAGCCTGGAA 
GAGAAGCTGC TGGTAGTTGG CACCCAGGAT GGCCTCATGG CTGTGTGGGA CATGGAAGAG 
CAGCAGGTGA TCCACATCCT AACTGGACAC ACAGGAGAGG TGAGGTCTGT GAAAGTGTTT 
GGCAAGGGGA CACTCGTCCT CTCTGCCTCA AAGGACCACA CACTGCGTTT GTGGAACTTA 
CTCTCTGGCC AGCAGAAATT CACCATTTGG GATGGAGGCT CAAAAGATCC CACTGACCCT 
GAGATCTGGA GTCTTCATGT GGATGAAGCA AAGAAGGTTG TGTATTTGGC ATCTGGCTCA 
AAGATCAGTG CTTGGAATCT GGAAACTGCA GAGCTGGTTT TCCATATCCT GGGAGATGCC 
TCCGACTGCT GGATGTGCAC AGCGGTGCTG GCTTCCCGGG CCACGCTGCT GACCGTGTCT 
GAGGGCGGTG TGGTCAGTCT GTGGAGCTCA GGCACAGGGA AACTGCAGGG GAGGCACCAT 
TTATCCAGCA TCAAAGAAGA AACACCTACC TGTGGGGTCT CGGTCCAGAA GCAAGGAAAG 
ATGGTGACTG GGTTCAGCAA AGGCTCCATC TCTTTGGTTT CCCCCGGAGG AGATAGCCTG 
CTGGAGAAGC TTCCAGAAGC CGTGAGGTTC CTGGTAGTCT CTGAAGATGA GTCCCTTCTC 
GCTGCAGGCG AGGAACAAGA TGCCCTGGAC ACCTCCAATG AGGTCAGGTG TCTGGAGGTT 
GCCCAGCAGG CCAATCTCCT TTTCACCGGC CTCGTTTCAG GGGTTGTCCT GGTGTTTCCC 
CTGAATTCCA GACAGGATGT GATGTGCATC CCCCCTCCCG AGGCCCGGAA AGCCATCAAC 
TGCATGGCCC TCAGTAAAGG CGAGGAGCGC CTGGCCATCG CCTATGACAG CATTGTCCTT 
GTGCTGGACA TCAGTCCCGG GGATCCCTGT CCGGTTATTG ATGGGCCAAC ATACACCTTC 
TATACCCAGC TGCCTGAGGC CATCTCCAGT GTGGCCGTTC TGGCTGACTA CCGCGTGATC 
TACGGCATGA CCAACGGTGA CCTCTTTCTT TACGAGTGTG CGAATTCCAA AGTGTTCCCT 
CTGGAGGCCC ACGGGAGCCA GGTCACCTGC ATGCAGGTCA GCCACAAGGA GCAGCTGGCT 
GTCAGCGGGT CCGAGGAAGC CCTGCTGTGT CTCTGGGACC TGCAGGCCTG CAAGTGGAGG 
TTCCAGATGA GCTACACGAA TTCTTACTGT AGAGGAGTCC AGTGTGTCTG CTTCTCCAAG 
GATGACAAGT ATGTGTATGC GGGCTTGAAG GATCGCTCCA TCATTGTCTG GAGTGTGCTG 
GATGGCACCC TGCTGGCTGT CCAGTTTGTC CATGCTGTGG TGAACAGGAT CATCCCAACT 
TCGAATGGCT TCATTGCCCC CACTAGACAT GGCTACCTGA TCCGCGAGCG TTTCCAGTGC 
CCTTCATCGA GAGTCTCACA GCAAGATCCT CTCAAGAACT TCAAGAAGGC AGTGTGGAAG 
GTCAAATCCA GGCAGAGAGA AGAGCTGGCA GCAGCTGCAG GAGCACTCCA GGACTCGAGA 
TCGGCAAGTG CCCAGGGAAA TGAACGTAAA TCGAACAAAC GCTCACAAGT CTGCCTGCTA 
GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022262626.1
CDS	253..4638
Translation	MSVGPLSPGTRQRLLQGQADPLPAPLSRSVAIFISSTVSDMDAEREALQSTAFPEVQTFCQKNGLMFEVVDLRWGIRNTEATDHMTTELCLEELDRCRKTSIGPAFVALLGDQYGPCPVPTLIEEKEWEALRSQLASKPRDLELVARRFRRDENAVPPTYLLQALGTAETQGPEEATLTSVLRSGAQEARRLGLITQEQWHRYHWSVIEWEIDRGLLSLTDGDQGATVFLREIQDLNKHILEDCALKMVDRLADGCLDTDAQNLLSGLKGRIADTQPGVLKAHHLPWSRDLVNPKNKAHARYLKELGEQFVARANHQVLEHLREQEAGRQELAWLYQEIRHHLGLSTEATWTFCGRQELLAQLGQRLRQSDGHPHSPLVLFGPPGIGKTALMCKLAEQMPGLLGRKTVTVLRLLGMSQMSSDARGLLQSICFQVCLAYGLPLPPAQVLEAHARVVQFFHTLLHTVSCRNFESLVILLDSMDDVDSICRARRVPWLPPRCPPRIHLILSACSGKPGILDIMRQTLTDPEAYWEVKPLSGNQGQEMIQLLLAASRRTLSPGQRELLWASLPECGHPGRLRLAFEEARKWASFTIPAPLASTAKEAIHQLCARLEQTHGQLLVAHVLSYIVSSRHGLSEAELKDVLSLDDEVLEAVYQDWTPPSKELLRFPPLLWVRLRRDLGHCLARRPVDGFTLLAIAHRQLTEVIQERYLSGPERAKRHGVLADFFSGAWSQGTKKLITLPLVGKPLNLDRKVAPQPLWFSDTVANLRKLKELPHHLVHSGRFEELKQEVLGSMNWISCRGVSGGIEGLLEDFDLYGPHLDCPEVGLVRDALQLCRPALELRGMERSILYTELLARLHFFATSHPALVGQLCQQAQSWFRLCPHPVLVPLGGFLQPPGGPLRATFTGCHKGITAMGWSLEEKLLVVGTQDGLMAVWDMEEQQVIHILTGHTGEVRSVKVFGKGTLVLSASKDHTLRLWNLLSGQQKFTIWDGGSKDPTDPEIWSLHVDEAKKVVYLASGSKISAWNLETAELVFHILGDASDCWMCTAVLASRATLLTVSEGGVVSLWSSGTGKLQGRHHLSSIKEETPTCGVSVQKQGKMVTGFSKGSISLVSPGGDSLLEKLPEAVRFLVVSEDESLLAAGEEQDALDTSNEVRCLEVAQQANLLFTGLVSGVVLVFPLNSRQDVMCIPPPEARKAINCMALSKGEERLAIAYDSIVLVLDISPGDPCPVIDGPTYTFYTQLPEAISSVAVLADYRVIYGMTNGDLFLYECANSKVFPLEAHGSQVTCMQVSHKEQLAVSGSEEALLCLWDLQACKWRFQMSYTNSYCRGVQCVCFSKDDKYVYAGLKDRSIIVWSVLDGTLLAVQFVHAVVNRIIPTSNGFIAPTRHGYLIRERFQCPSSRVSQQDPLKNFKKAVWKVKSRQREELAAAAGALQDSRSASAQGNERKSNKRSQVCLLV

Target ORF information:

RefSeq Version	XM_022406918.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris NACHT and WD repeat domain containing 1 (NWD1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022406918.1

ORF Insert Sequence:

ATGAGTGTGG GCCCCCTCAG CCCGGGCACC CGCCAGCGAC TTCTCCAGGG TCAGGCAGAC 
CCCCTCCCAG CGCCTCTGTC CAGAAGTGTG GCCATCTTCA TCAGCTCCAC GGTCTCGGAT 
ATGGATGCAG AGAGAGAAGC TCTGCAGAGC ACTGCCTTCC CCGAAGTGCA GACCTTCTGC 
CAAAAGAATG GCCTGATGTT CGAGGTTGTT GACCTGAGGT GGGGTATTCG GAACACTGAG 
GCCACTGACC ACATGACCAC AGAACTCTGC TTGGAGGAGC TCGACCGGTG TCGGAAAACA 
TCCATAGGGC CGGCTTTTGT TGCCCTCTTA GGTGACCAGT ACGGCCCCTG TCCAGTCCCT 
ACACTGATCG AGGAGAAGGA GTGGGAGGCC CTGAGATCCC AGCTGGCCTC CAAGCCACGT 
GACCTGGAGC TGGTGGCTCG ACGCTTCCGG AGGGATGAGA ACGCCGTGCC CCCCACCTAC 
CTGCTTCAGG CCCTAGGCAC TGCGGAGACC CAGGGGCCCG AAGAGGCCAC CCTGACCTCT 
GTGCTGCGCT CCGGAGCCCA GGAGGCCCGG AGGCTGGGCC TCATCACCCA GGAGCAGTGG 
CACCGCTACC ACTGGTCAGT CATTGAGTGG GAGATAGACC GGGGCCTGCT GAGCTTGACA 
GACGGAGACC AGGGGGCGAC TGTCTTCTTG AGAGAGATCC AAGACCTCAA CAAACACATC 
CTGGAAGACT GCGCCCTCAA GATGGTGGAC CGGCTCGCAG ATGGCTGCCT GGACACAGAT 
GCCCAGAACC TTCTCAGTGG CCTCAAGGGA CGCATTGCCG ACACACAACC TGGAGTCCTC 
AAGGCCCACC ACCTGCCATG GAGCCGAGAC CTGGTGAACC CCAAAAACAA GGCTCATGCT 
CGGTACCTGA AAGAGCTGGG GGAGCAGTTT GTGGCCAGGG CTAACCACCA GGTCCTTGAG 
CACCTCCGGG AGCAGGAGGC AGGCAGGCAG GAGTTGGCGT GGCTCTACCA GGAGATCCGC 
CACCACCTGG GGCTGAGCAC CGAGGCCACC TGGACCTTTT GTGGCCGCCA GGAGCTCCTG 
GCCCAGCTGG GGCAGCGGCT CCGGCAGAGC GATGGCCATC CGCACTCACC CCTGGTGCTT 
TTTGGACCTC CAGGCATCGG AAAGACAGCG CTGATGTGCA AGTTGGCAGA ACAAATGCCG 
GGACTGCTTG GCCGCAAGAC AGTGACCGTC CTGCGGCTTC TGGGGATGTC ACAGATGAGC 
TCCGACGCCC GAGGCCTGCT CCAGAGCATC TGCTTCCAGG TGTGCCTGGC CTACGGGCTG 
CCCCTGCCCC CTGCCCAGGT CCTGGAGGCC CATGCCAGGG TGGTCCAGTT TTTCCACACC 
CTCCTCCACA CTGTCTCCTG CCGAAACTTC GAGTCACTTG TGATCCTGTT GGACTCCATG 
GATGATGTGG ATTCCATCTG CCGCGCCCGG AGGGTCCCCT GGCTGCCTCC CCGGTGCCCC 
CCGAGGATCC ACCTTATCCT CTCAGCCTGC TCGGGGAAGC CGGGGATTCT AGACATCATG 
AGACAGACGC TGACGGACCC AGAGGCCTAC TGGGAGGTGA AACCCCTCTC CGGAAACCAA 
GGGCAGGAGA TGATCCAGCT CCTGCTGGCC GCCTCGCGGA GGACGCTGAG CCCAGGGCAG 
AGGGAGCTGC TCTGGGCCAG CCTCCCAGAG TGTGGGCACC CAGGACGGCT GAGGCTGGCC 
TTTGAGGAGG CCCGGAAGTG GGCCTCCTTC ACCATACCTG CCCCTCTGGC CTCCACTGCT 
AAGGAAGCGA TCCATCAGCT GTGTGCCCGC CTGGAGCAGA CACACGGGCA GCTCCTGGTG 
GCCCACGTGC TGAGCTACAT CGTGTCCTCC CGGCATGGGC TCTCAGAGGC AGAGCTGAAG 
GACGTCCTGT CCTTGGACGA CGAGGTCCTG GAGGCGGTGT ACCAGGACTG GACCCCGCCC 
AGCAAGGAGC TGTTGCGCTT CCCCCCGTTG CTGTGGGTGA GGCTCCGTCG GGATCTCGGC 
CACTGCTTGG CCCGGCGGCC GGTGGACGGC TTCACGCTCC TGGCCATCGC CCACAGGCAG 
CTGACTGAGG TGATCCAAGA GCGCTACCTG TCTGGGCCCG AGAGAGCCAA GAGGCACGGA 
GTCTTGGCTG ACTTCTTCTC AGGGGCCTGG AGCCAGGGCA CAAAGAAACT CATCACCCTG 
CCACTCGTGG GGAAGCCCCT GAACCTGGAC CGCAAGGTGG CGCCTCAGCC CCTGTGGTTC 
TCAGACACAG TTGCAAATCT GCGGAAGCTG AAGGAGTTGC CCCATCACCT GGTCCACTCA 
GGCCGCTTCG AAGAGCTGAA GCAGGAGGTT CTGGGCAGCA TGAACTGGAT TTCCTGCCGG 
GGCGTCTCTG GGGGCATCGA GGGGCTGTTG GAGGACTTTG ACCTGTATGG CCCTCACCTG 
GATTGTCCCG AGGTCGGCCT GGTCCGGGAC GCCCTCCAGC TGTGCCGCCC GGCCCTGGAG 
CTCCGAGGCA TGGAGAGGAG CATCCTCTAC ACTGAACTCC TGGCCAGACT CCATTTCTTC 
GCCACCTCAC ATCCAGCGCT GGTGGGACAG CTGTGCCAAC AGGCCCAGAG CTGGTTCCGG 
CTGTGCCCCC ACCCTGTGCT GGTACCTCTC GGGGGGTTCC TCCAGCCCCC CGGGGGACCC 
CTCCGGGCAA CCTTCACTGG CTGTCACAAG GGCATCACTG CCATGGGCTG GAGCCTGGAA 
GAGAAGCTGC TGGTAGTTGG CACCCAGGAT GGCCTCATGG CTGTGTGGGA CATGGAAGAG 
CAGCAGGTGA TCCACATCCT AACTGGACAC ACAGGAGAGG TGAGGTCTGT GAAAGTGTTT 
GGCAAGGGGA CACTCGTCCT CTCTGCCTCA AAGGACCACA CACTGCGTTT GTGGAACTTA 
CTCTCTGGCC AGCAGAAATT CACCATTTGG GATGGAGGCT CAAAAGATCC CACTGACCCT 
GAGATCTGGA GTCTTCATGT GGATGAAGCA AAGAAGGTTG TGTATTTGGC ATCTGGCTCA 
AAGATCAGTG CTTGGAATCT GGAAACTGCA GAGCTGGTTT TCCATATCCT GGGAGATGCC 
TCCGACTGCT GGATGTGCAC AGCGGTGCTG GCTTCCCGGG CCACGCTGCT GACCGTGTCT 
GAGGGCGGTG TGGTCAGTCT GTGGAGCTCA GGCACAGGGA AACTGCAGGG GAGGCACCAT 
TTATCCAGCA TCAAAGAAGA AACACCTACC TGTGGGGTCT CGGTCCAGAA GCAAGGAAAG 
ATGGTGACTG GGTTCAGCAA AGGCTCCATC TCTTTGGTTT CCCCCGGAGG AGATAGCCTG 
CTGGAGAAGC TTCCAGAAGC CGTGAGGTTC CTGGTAGTCT CTGAAGATGA GTCCCTTCTC 
GCTGCAGGCG AGGAACAAGA TGCCCTGGAC ACCTCCAATG AGGTCAGGTG TCTGGAGGTT 
GCCCAGCAGG CCAATCTCCT TTTCACCGGC CTCGTTTCAG GGGTTGTCCT GGTGTTTCCC 
CTGAATTCCA GACAGGATGT GATGTGCATC CCCCCTCCCG AGGCCCGGAA AGCCATCAAC 
TGCATGGCCC TCAGTAAAGG CGAGGAGCGC CTGGCCATCG CCTATGACAG CATTGTCCTT 
GTGCTGGACA TCAGTCCCGG GGATCCCTGT CCGGTTATTG ATGGGCCAAC ATACACCTTC 
TATACCCAGC TGCCTGAGGC CATCTCCAGT GTGGCCGTTC TGGCTGACTA CCGCGTGATC 
TACGGCATGA CCAACGGTGA CCTCTTTCTT TACGAGTGTG CGAATTCCAA AGTGTTCCCT 
CTGGAGGCCC ACGGGAGCCA GGTCACCTGC ATGCAGGTCA GCCACAAGGA GCAGCTGGCT 
GTCAGCGGGT CCGAGGAAGC CCTGCTGTGT CTCTGGGACC TGCAGGCCTG CAAGTGGAGG 
TTCCAGATGA GCTACACGAA TTCTTACTGT AGAGGAGTCC AGTGTGTCTG CTTCTCCAAG 
GATGACAAGT ATGTGTATGC GGGCTTGAAG GATCGCTCCA TCATTGTCTG GAGTGTGCTG 
GATGGCACCC TGCTGGCTGT CCAGTTTGTC CATGCTGTGG TGAACAGGAT CATCCCAACT 
TCGAATGGCT TCATTGCCCC CACTAGACAT GGCTACCTGA TCCGCGAGCG TTTCCAGTGC 
CCTTCATCGA GAGTCTCACA GCAAGATCCT CTCAAGAACT TCAAGAAGGC AGTGTGGAAG 
GTCAAATCCA GGCAGAGAGA AGAGCTGGCA GCAGCTGCAG GAGCACTCCA GGACTCGAGA 
TCGGCAAGTG CCCAGGGAAA TGAACGTAAA TCGAACAAAC GCTCACAAGT CTGCCTGCTA 
GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NWD1 ( XM_022406919.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022262627.1 Canis lupus familiaris NACHT and WD repeat domain containing 1 (NWD1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe295551
Clone ID Related Accession (Same CDS sequence)	XM_022406919.1
Accession Version	XM_022406919.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	NACHT domain- and WD repeat-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCTT GGGCTGTTTC CACCTTTTGG CTACTGTGGA TAATACTGCT GGGAACTTGG 
GTCACAGTCA TTGAGTGGGA GATAGACCGG GGCCTGCTGA GCTTGACAGA CGGAGACCAG 
GGGGCGACTG TCTTCTTGAG AGAGATCCAA GACCTCAACA AACACATCCT GGAAGACTGC 
GCCCTCAAGA TGGTGGACCG GCTCGCAGAT GGCTGCCTGG ACACAGATGC CCAGAACCTT 
CTCAGTGGCC TCAAGGGACG CATTGCCGAC ACACAACCTG GAGTCCTCAA GGCCCACCAC 
CTGCCATGGA GCCGAGACCT GGTGAACCCC AAAAACAAGG CTCATGCTCG GTACCTGAAA 
GAGCTGGGGG AGCAGTTTGT GGCCAGGGCT AACCACCAGG TCCTTGAGCA CCTCCGGGAG 
CAGGAGGCAG GCAGGCAGGA GTTGGCGTGG CTCTACCAGG AGATCCGCCA CCACCTGGGG 
CTGAGCACCG AGGCCACCTG GACCTTTTGT GGCCGCCAGG AGCTCCTGGC CCAGCTGGGG 
CAGCGGCTCC GGCAGAGCGA TGGCCATCCG CACTCACCCC TGGTGCTTTT TGGACCTCCA 
GGCATCGGAA AGACAGCGCT GATGTGCAAG TTGGCAGAAC AAATGCCGGG ACTGCTTGGC 
CGCAAGACAG TGACCGTCCT GCGGCTTCTG GGGATGTCAC AGATGAGCTC CGACGCCCGA 
GGCCTGCTCC AGAGCATCTG CTTCCAGGTG TGCCTGGCCT ACGGGCTGCC CCTGCCCCCT 
GCCCAGGTCC TGGAGGCCCA TGCCAGGGTG GTCCAGTTTT TCCACACCCT CCTCCACACT 
GTCTCCTGCC GAAACTTCGA GTCACTTGTG ATCCTGTTGG ACTCCATGGA TGATGTGGAT 
TCCATCTGCC GCGCCCGGAG GGTCCCCTGG CTGCCTCCCC GGTGCCCCCC GAGGATCCAC 
CTTATCCTCT CAGCCTGCTC GGGGAAGCCG GGGATTCTAG ACATCATGAG ACAGACGCTG 
ACGGACCCAG AGGCCTACTG GGAGGTGAAA CCCCTCTCCG GAAACCAAGG GCAGGAGATG 
ATCCAGCTCC TGCTGGCCGC CTCGCGGAGG ACGCTGAGCC CAGGGCAGAG GGAGCTGCTC 
TGGGCCAGCC TCCCAGAGTG TGGGCACCCA GGACGGCTGA GGCTGGCCTT TGAGGAGGCC 
CGGAAGTGGG CCTCCTTCAC CATACCTGCC CCTCTGGCCT CCACTGCTAA GGAAGCGATC 
CATCAGCTGT GTGCCCGCCT GGAGCAGACA CACGGGCAGC TCCTGGTGGC CCACGTGCTG 
AGCTACATCG TGTCCTCCCG GCATGGGCTC TCAGAGGCAG AGCTGAAGGA CGTCCTGTCC 
TTGGACGACG AGGTCCTGGA GGCGGTGTAC CAGGACTGGA CCCCGCCCAG CAAGGAGCTG 
TTGCGCTTCC CCCCGTTGCT GTGGGTGAGG CTCCGTCGGG ATCTCGGCCA CTGCTTGGCC 
CGGCGGCCGG TGGACGGCTT CACGCTCCTG GCCATCGCCC ACAGGCAGCT GACTGAGGTG 
ATCCAAGAGC GCTACCTGTC TGGGCCCGAG AGAGCCAAGA GGCACGGAGT CTTGGCTGAC 
TTCTTCTCAG GGGCCTGGAG CCAGGGCACA AAGAAACTCA TCACCCTGCC ACTCGTGGGG 
AAGCCCCTGA ACCTGGACCG CAAGGTGGCG CCTCAGCCCC TGTGGTTCTC AGACACAGTT 
GCAAATCTGC GGAAGCTGAA GGAGTTGCCC CATCACCTGG TCCACTCAGG CCGCTTCGAA 
GAGCTGAAGC AGGAGGTTCT GGGCAGCATG AACTGGATTT CCTGCCGGGG CGTCTCTGGG 
GGCATCGAGG GGCTGTTGGA GGACTTTGAC CTGTATGGCC CTCACCTGGA TTGTCCCGAG 
GTCGGCCTGG TCCGGGACGC CCTCCAGCTG TGCCGCCCGG CCCTGGAGCT CCGAGGCATG 
GAGAGGAGCA TCCTCTACAC TGAACTCCTG GCCAGACTCC ATTTCTTCGC CACCTCACAT 
CCAGCGCTGG TGGGACAGCT GTGCCAACAG GCCCAGAGCT GGTTCCGGCT GTGCCCCCAC 
CCTGTGCTGG TACCTCTCGG GGGGTTCCTC CAGCCCCCCG GGGGACCCCT CCGGGCAACC 
TTCACTGGCT GTCACAAGGG CATCACTGCC ATGGGCTGGA GCCTGGAAGA GAAGCTGCTG 
GTAGTTGGCA CCCAGGATGG CCTCATGGCT GTGTGGGACA TGGAAGAGCA GCAGGTGATC 
CACATCCTAA CTGGACACAC AGGAGAGGTG AGGTCTGTGA AAGTGTTTGG CAAGGGGACA 
CTCGTCCTCT CTGCCTCAAA GGACCACACA CTGCGTTTGT GGAACTTACT CTCTGGCCAG 
CAGAAATTCA CCATTTGGGA TGGAGGCTCA AAAGATCCCA CTGACCCTGA GATCTGGAGT 
CTTCATGTGG ATGAAGCAAA GAAGGTTGTG TATTTGGCAT CTGGCTCAAA GATCAGTGCT 
TGGAATCTGG AAACTGCAGA GCTGGTTTTC CATATCCTGG GAGATGCCTC CGACTGCTGG 
ATGTGCACAG CGGTGCTGGC TTCCCGGGCC ACGCTGCTGA CCGTGTCTGA GGGCGGTGTG 
GTCAGTCTGT GGAGCTCAGG CACAGGGAAA CTGCAGGGGA GGCACCATTT ATCCAGCATC 
AAAGAAGAAA CACCTACCTG TGGGGTCTCG GTCCAGAAGC AAGGAAAGAT GGTGACTGGG 
TTCAGCAAAG GCTCCATCTC TTTGGTTTCC CCCGGAGGAG ATAGCCTGCT GGAGAAGCTT 
CCAGAAGCCG TGAGGTTCCT GGTAGTCTCT GAAGATGAGT CCCTTCTCGC TGCAGGGTTT 
GGCAGATCCG TGCGGATATT CTTGGCAGAC TCGCAGGGGT TTCATCGATT TATGGCCACC 
GACCTTGAAC ATGAGGACAC CGTGGAGATA GCTGGTTTTG GTCCTGAGAA CAATCTGATT 
GTCACTGCGT CCCGCGATGC ACTCATCCAG GTGTGGAGCC TGTCGGAGCA GGGGACCCTG 
CTGGACATCC TGGAAGGTGT CGGAGCCCCC GTGAGCCTGC TGGCCTGGGC TGGGGCCCTG 
GTGGCATCTG CTTCTCAGCA GTCCTCATCT TTGAAAGTCT GGGATCTCAC TTACATTCAG 
AAGCCGAGGG CCTGCACCCC ATTTCTGGAT CGTACTGGCC TCACTGCTGT GTCACACAAT 
GGAAGCTACG TTTACTTCCC CAAAATCGGA GACAAGAACA AAGTTACCAT TTGGGACTCT 
GCAGAAGGCG AGGAACAAGA TGCCCTGGAC ACCTCCAATG AGGTCAGGTG TCTGGAGGTT 
GCCCAGCAGG CCAATCTCCT TTTCACCGGC CTCGTTTCAG GGGTTGTCCT GGTGTTTCCC 
CTGAATTCCA GACAGGATGT GATGTGCATC CCCCCTCCCG AGGCCCGGAA AGCCATCAAC 
TGCATGGCCC TCAGTAAAGG CGAGGAGCGC CTGGCCATCG CCTATGACAG CATTGTCCTT 
GTGCTGGACA TCAGTCCCGG GGATCCCTGT CCGGTTATTG ATGGGCCAAC ATACACCTTC 
TATACCCAGC TGCCTGAGGC CATCTCCAGT GTGGCCGTTC TGGCTGACTA CCGCGTGATC 
TACGGCATGA CCAACGGTGA CCTCTTTCTT TACGAGTGTG CGAATTCCAA AGTGTTCCCT 
CTGGAGGCCC ACGGGAGCCA GGTCACCTGC ATGCAGGTCA GCCACAAGGA GCAGCTGGCT 
GTCAGCGGGT CCGAGGAAGC CCTGCTGTGT CTCTGGGACC TGCAGGCCTG CAAGTGGAGG 
TTCCAGATGA GCTACACGAA TTCTTACTGT AGAGGAGTCC AGTGTGTCTG CTTCTCCAAG 
GATGACAAGT ATGTGTATGC GGGCTTGAAG GATCGCTCCA TCATTGTCTG GAGTGTGCTG 
GATGGCACCC TGCTGGCTGT CCAGTTTGTC CATGCTGTGG TGAACAGGAT CATCCCAACT 
TCGAATGGCT TCATTGCCCC CACTAGACAT GGCTACCTGA TCCGCGAGCG TTTCCAGTGC 
CCTTCATCGA GAGTCTCACA GCAAGATCCT CTCAAGAACT TCAAGAAGGC AGTGTGGAAG 
GTCAAATCCA GGCAGAGAGA AGAGCTGGCA GCAGCTGCAG GAGCACTCCA GGACTCGAGA 
TCGGCAAGTG CCCAGGGAAA TGAACGTAAA TCGAACAAAC GCTCACAAGT CTGCCTGCTA 
GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022262627.1
CDS	63..4328
Translation	MDPWAVSTFWLLWIILLGTWVTVIEWEIDRGLLSLTDGDQGATVFLREIQDLNKHILEDCALKMVDRLADGCLDTDAQNLLSGLKGRIADTQPGVLKAHHLPWSRDLVNPKNKAHARYLKELGEQFVARANHQVLEHLREQEAGRQELAWLYQEIRHHLGLSTEATWTFCGRQELLAQLGQRLRQSDGHPHSPLVLFGPPGIGKTALMCKLAEQMPGLLGRKTVTVLRLLGMSQMSSDARGLLQSICFQVCLAYGLPLPPAQVLEAHARVVQFFHTLLHTVSCRNFESLVILLDSMDDVDSICRARRVPWLPPRCPPRIHLILSACSGKPGILDIMRQTLTDPEAYWEVKPLSGNQGQEMIQLLLAASRRTLSPGQRELLWASLPECGHPGRLRLAFEEARKWASFTIPAPLASTAKEAIHQLCARLEQTHGQLLVAHVLSYIVSSRHGLSEAELKDVLSLDDEVLEAVYQDWTPPSKELLRFPPLLWVRLRRDLGHCLARRPVDGFTLLAIAHRQLTEVIQERYLSGPERAKRHGVLADFFSGAWSQGTKKLITLPLVGKPLNLDRKVAPQPLWFSDTVANLRKLKELPHHLVHSGRFEELKQEVLGSMNWISCRGVSGGIEGLLEDFDLYGPHLDCPEVGLVRDALQLCRPALELRGMERSILYTELLARLHFFATSHPALVGQLCQQAQSWFRLCPHPVLVPLGGFLQPPGGPLRATFTGCHKGITAMGWSLEEKLLVVGTQDGLMAVWDMEEQQVIHILTGHTGEVRSVKVFGKGTLVLSASKDHTLRLWNLLSGQQKFTIWDGGSKDPTDPEIWSLHVDEAKKVVYLASGSKISAWNLETAELVFHILGDASDCWMCTAVLASRATLLTVSEGGVVSLWSSGTGKLQGRHHLSSIKEETPTCGVSVQKQGKMVTGFSKGSISLVSPGGDSLLEKLPEAVRFLVVSEDESLLAAGFGRSVRIFLADSQGFHRFMATDLEHEDTVEIAGFGPENNLIVTASRDALIQVWSLSEQGTLLDILEGVGAPVSLLAWAGALVASASQQSSSLKVWDLTYIQKPRACTPFLDRTGLTAVSHNGSYVYFPKIGDKNKVTIWDSAEGEEQDALDTSNEVRCLEVAQQANLLFTGLVSGVVLVFPLNSRQDVMCIPPPEARKAINCMALSKGEERLAIAYDSIVLVLDISPGDPCPVIDGPTYTFYTQLPEAISSVAVLADYRVIYGMTNGDLFLYECANSKVFPLEAHGSQVTCMQVSHKEQLAVSGSEEALLCLWDLQACKWRFQMSYTNSYCRGVQCVCFSKDDKYVYAGLKDRSIIVWSVLDGTLLAVQFVHAVVNRIIPTSNGFIAPTRHGYLIRERFQCPSSRVSQQDPLKNFKKAVWKVKSRQREELAAAAGALQDSRSASAQGNERKSNKRSQVCLLV

Target ORF information:

RefSeq Version	XM_022406919.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris NACHT and WD repeat domain containing 1 (NWD1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022406919.1

ORF Insert Sequence:

ATGGACCCTT GGGCTGTTTC CACCTTTTGG CTACTGTGGA TAATACTGCT GGGAACTTGG 
GTCACAGTCA TTGAGTGGGA GATAGACCGG GGCCTGCTGA GCTTGACAGA CGGAGACCAG 
GGGGCGACTG TCTTCTTGAG AGAGATCCAA GACCTCAACA AACACATCCT GGAAGACTGC 
GCCCTCAAGA TGGTGGACCG GCTCGCAGAT GGCTGCCTGG ACACAGATGC CCAGAACCTT 
CTCAGTGGCC TCAAGGGACG CATTGCCGAC ACACAACCTG GAGTCCTCAA GGCCCACCAC 
CTGCCATGGA GCCGAGACCT GGTGAACCCC AAAAACAAGG CTCATGCTCG GTACCTGAAA 
GAGCTGGGGG AGCAGTTTGT GGCCAGGGCT AACCACCAGG TCCTTGAGCA CCTCCGGGAG 
CAGGAGGCAG GCAGGCAGGA GTTGGCGTGG CTCTACCAGG AGATCCGCCA CCACCTGGGG 
CTGAGCACCG AGGCCACCTG GACCTTTTGT GGCCGCCAGG AGCTCCTGGC CCAGCTGGGG 
CAGCGGCTCC GGCAGAGCGA TGGCCATCCG CACTCACCCC TGGTGCTTTT TGGACCTCCA 
GGCATCGGAA AGACAGCGCT GATGTGCAAG TTGGCAGAAC AAATGCCGGG ACTGCTTGGC 
CGCAAGACAG TGACCGTCCT GCGGCTTCTG GGGATGTCAC AGATGAGCTC CGACGCCCGA 
GGCCTGCTCC AGAGCATCTG CTTCCAGGTG TGCCTGGCCT ACGGGCTGCC CCTGCCCCCT 
GCCCAGGTCC TGGAGGCCCA TGCCAGGGTG GTCCAGTTTT TCCACACCCT CCTCCACACT 
GTCTCCTGCC GAAACTTCGA GTCACTTGTG ATCCTGTTGG ACTCCATGGA TGATGTGGAT 
TCCATCTGCC GCGCCCGGAG GGTCCCCTGG CTGCCTCCCC GGTGCCCCCC GAGGATCCAC 
CTTATCCTCT CAGCCTGCTC GGGGAAGCCG GGGATTCTAG ACATCATGAG ACAGACGCTG 
ACGGACCCAG AGGCCTACTG GGAGGTGAAA CCCCTCTCCG GAAACCAAGG GCAGGAGATG 
ATCCAGCTCC TGCTGGCCGC CTCGCGGAGG ACGCTGAGCC CAGGGCAGAG GGAGCTGCTC 
TGGGCCAGCC TCCCAGAGTG TGGGCACCCA GGACGGCTGA GGCTGGCCTT TGAGGAGGCC 
CGGAAGTGGG CCTCCTTCAC CATACCTGCC CCTCTGGCCT CCACTGCTAA GGAAGCGATC 
CATCAGCTGT GTGCCCGCCT GGAGCAGACA CACGGGCAGC TCCTGGTGGC CCACGTGCTG 
AGCTACATCG TGTCCTCCCG GCATGGGCTC TCAGAGGCAG AGCTGAAGGA CGTCCTGTCC 
TTGGACGACG AGGTCCTGGA GGCGGTGTAC CAGGACTGGA CCCCGCCCAG CAAGGAGCTG 
TTGCGCTTCC CCCCGTTGCT GTGGGTGAGG CTCCGTCGGG ATCTCGGCCA CTGCTTGGCC 
CGGCGGCCGG TGGACGGCTT CACGCTCCTG GCCATCGCCC ACAGGCAGCT GACTGAGGTG 
ATCCAAGAGC GCTACCTGTC TGGGCCCGAG AGAGCCAAGA GGCACGGAGT CTTGGCTGAC 
TTCTTCTCAG GGGCCTGGAG CCAGGGCACA AAGAAACTCA TCACCCTGCC ACTCGTGGGG 
AAGCCCCTGA ACCTGGACCG CAAGGTGGCG CCTCAGCCCC TGTGGTTCTC AGACACAGTT 
GCAAATCTGC GGAAGCTGAA GGAGTTGCCC CATCACCTGG TCCACTCAGG CCGCTTCGAA 
GAGCTGAAGC AGGAGGTTCT GGGCAGCATG AACTGGATTT CCTGCCGGGG CGTCTCTGGG 
GGCATCGAGG GGCTGTTGGA GGACTTTGAC CTGTATGGCC CTCACCTGGA TTGTCCCGAG 
GTCGGCCTGG TCCGGGACGC CCTCCAGCTG TGCCGCCCGG CCCTGGAGCT CCGAGGCATG 
GAGAGGAGCA TCCTCTACAC TGAACTCCTG GCCAGACTCC ATTTCTTCGC CACCTCACAT 
CCAGCGCTGG TGGGACAGCT GTGCCAACAG GCCCAGAGCT GGTTCCGGCT GTGCCCCCAC 
CCTGTGCTGG TACCTCTCGG GGGGTTCCTC CAGCCCCCCG GGGGACCCCT CCGGGCAACC 
TTCACTGGCT GTCACAAGGG CATCACTGCC ATGGGCTGGA GCCTGGAAGA GAAGCTGCTG 
GTAGTTGGCA CCCAGGATGG CCTCATGGCT GTGTGGGACA TGGAAGAGCA GCAGGTGATC 
CACATCCTAA CTGGACACAC AGGAGAGGTG AGGTCTGTGA AAGTGTTTGG CAAGGGGACA 
CTCGTCCTCT CTGCCTCAAA GGACCACACA CTGCGTTTGT GGAACTTACT CTCTGGCCAG 
CAGAAATTCA CCATTTGGGA TGGAGGCTCA AAAGATCCCA CTGACCCTGA GATCTGGAGT 
CTTCATGTGG ATGAAGCAAA GAAGGTTGTG TATTTGGCAT CTGGCTCAAA GATCAGTGCT 
TGGAATCTGG AAACTGCAGA GCTGGTTTTC CATATCCTGG GAGATGCCTC CGACTGCTGG 
ATGTGCACAG CGGTGCTGGC TTCCCGGGCC ACGCTGCTGA CCGTGTCTGA GGGCGGTGTG 
GTCAGTCTGT GGAGCTCAGG CACAGGGAAA CTGCAGGGGA GGCACCATTT ATCCAGCATC 
AAAGAAGAAA CACCTACCTG TGGGGTCTCG GTCCAGAAGC AAGGAAAGAT GGTGACTGGG 
TTCAGCAAAG GCTCCATCTC TTTGGTTTCC CCCGGAGGAG ATAGCCTGCT GGAGAAGCTT 
CCAGAAGCCG TGAGGTTCCT GGTAGTCTCT GAAGATGAGT CCCTTCTCGC TGCAGGGTTT 
GGCAGATCCG TGCGGATATT CTTGGCAGAC TCGCAGGGGT TTCATCGATT TATGGCCACC 
GACCTTGAAC ATGAGGACAC CGTGGAGATA GCTGGTTTTG GTCCTGAGAA CAATCTGATT 
GTCACTGCGT CCCGCGATGC ACTCATCCAG GTGTGGAGCC TGTCGGAGCA GGGGACCCTG 
CTGGACATCC TGGAAGGTGT CGGAGCCCCC GTGAGCCTGC TGGCCTGGGC TGGGGCCCTG 
GTGGCATCTG CTTCTCAGCA GTCCTCATCT TTGAAAGTCT GGGATCTCAC TTACATTCAG 
AAGCCGAGGG CCTGCACCCC ATTTCTGGAT CGTACTGGCC TCACTGCTGT GTCACACAAT 
GGAAGCTACG TTTACTTCCC CAAAATCGGA GACAAGAACA AAGTTACCAT TTGGGACTCT 
GCAGAAGGCG AGGAACAAGA TGCCCTGGAC ACCTCCAATG AGGTCAGGTG TCTGGAGGTT 
GCCCAGCAGG CCAATCTCCT TTTCACCGGC CTCGTTTCAG GGGTTGTCCT GGTGTTTCCC 
CTGAATTCCA GACAGGATGT GATGTGCATC CCCCCTCCCG AGGCCCGGAA AGCCATCAAC 
TGCATGGCCC TCAGTAAAGG CGAGGAGCGC CTGGCCATCG CCTATGACAG CATTGTCCTT 
GTGCTGGACA TCAGTCCCGG GGATCCCTGT CCGGTTATTG ATGGGCCAAC ATACACCTTC 
TATACCCAGC TGCCTGAGGC CATCTCCAGT GTGGCCGTTC TGGCTGACTA CCGCGTGATC 
TACGGCATGA CCAACGGTGA CCTCTTTCTT TACGAGTGTG CGAATTCCAA AGTGTTCCCT 
CTGGAGGCCC ACGGGAGCCA GGTCACCTGC ATGCAGGTCA GCCACAAGGA GCAGCTGGCT 
GTCAGCGGGT CCGAGGAAGC CCTGCTGTGT CTCTGGGACC TGCAGGCCTG CAAGTGGAGG 
TTCCAGATGA GCTACACGAA TTCTTACTGT AGAGGAGTCC AGTGTGTCTG CTTCTCCAAG 
GATGACAAGT ATGTGTATGC GGGCTTGAAG GATCGCTCCA TCATTGTCTG GAGTGTGCTG 
GATGGCACCC TGCTGGCTGT CCAGTTTGTC CATGCTGTGG TGAACAGGAT CATCCCAACT 
TCGAATGGCT TCATTGCCCC CACTAGACAT GGCTACCTGA TCCGCGAGCG TTTCCAGTGC 
CCTTCATCGA GAGTCTCACA GCAAGATCCT CTCAAGAACT TCAAGAAGGC AGTGTGGAAG 
GTCAAATCCA GGCAGAGAGA AGAGCTGGCA GCAGCTGCAG GAGCACTCCA GGACTCGAGA 
TCGGCAAGTG CCCAGGGAAA TGAACGTAAA TCGAACAAAC GCTCACAAGT CTGCCTGCTA 
GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NWD1 ( XM_022406922.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022262630.1 Canis lupus familiaris NACHT and WD repeat domain containing 1 (NWD1), transcript variant X7, mRNA.

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CloneID	OCe295552
Clone ID Related Accession (Same CDS sequence)	XM_022406922.1
Accession Version	XM_022406922.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	NACHT domain- and WD repeat-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGG GCCCCCTCAG CCCGGGCACC CGCCAGCGAC TTCTCCAGGG TCAGGCAGAC 
CCCCTCCCAG CGCCTCTGTC CAGAAGTGTG GCCATCTTCA TCAGCTCCAC GGTCTCGGAT 
ATGGATGCAG AGAGAGAAGC TCTGCAGAGC ACTGCCTTCC CCGAAGTGCA GACCTTCTGC 
CAAAAGAATG GCCTGATGTT CGAGGTTGTT GACCTGAGGT GGGGTATTCG GAACACTGAG 
GCCACTGACC ACATGACCAC AGAACTCTGC TTGGAGGAGC TCGACCGGTG TCGGAAAACA 
TCCATAGGGC CGGCTTTTGT TGCCCTCTTA GGTGACCAGT ACGGCCCCTG TCCAGTCCCT 
ACACTGATCG AGGAGAAGGA GTGGGAGGCC CTGAGATCCC AGCTGGCCTC CAAGCCACGT 
GACCTGGAGC TGGTGGCTCG ACGCTTCCGG AGGGATGAGA ACGCCGTGCC CCCCACCTAC 
CTGCTTCAGG CCCTAGGCAC TGCGGAGACC CAGGGGCCCG AAGAGGCCAC CCTGACCTCT 
GTGCTGCGCT CCGGAGCCCA GGAGGCCCGG AGGCTGGGCC TCATCACCCA GGAGCAGTGG 
CACCGCTACC ACTGGTCAGT CATTGAGTGG GAGATAGACC GGGGCCTGCT GAGCTTGACA 
GACGGAGACC AGGGGGCGAC TGTCTTCTTG AGAGAGATCC AAGACCTCAA CAAACACATC 
CTGGAAGACT GCGCCCTCAA GATGGTGGAC CGGCTCGCAG ATGGCTGCCT GGACACAGAT 
GCCCAGAACC TTCTCAGTGG CCTCAAGGGA CGCATTGCCG ACACACAACC TGGAGTCCTC 
AAGGCCCACC ACCTGCCATG GAGCCGAGAC CTGGTGAACC CCAAAAACAA GGCTCATGCT 
CGGTACCTGA AAGAGCTGGG GGAGCAGTTT GTGGCCAGGG CTAACCACCA GGTCCTTGAG 
CACCTCCGGG AGCAGGAGGC AGGCAGGCAG GAGTTGGCGT GGCTCTACCA GGAGATCCGC 
CACCACCTGG GGCTGAGCAC CGAGGCCACC TGGACCTTTT GTGGCCGCCA GGAGCTCCTG 
GCCCAGCTGG GGCAGCGGCT CCGGCAGAGC GATGGCCATC CGCACTCACC CCTGGTGCTT 
TTTGGACCTC CAGGCATCGG AAAGACAGCG CTGATGTGCA AGTTGGCAGA ACAAATGCCG 
GGACTGCTTG GCCGCAAGAC AGTGACCGTC CTGCGGCTTC TGGGGATGTC ACAGATGAGC 
TCCGACGCCC GAGGCCTGCT CCAGAGCATC TGCTTCCAGG TGTGCCTGGC CTACGGGCTG 
CCCCTGCCCC CTGCCCAGGT CCTGGAGGCC CATGCCAGGG TGGTCCAGTT TTTCCACACC 
CTCCTCCACA CTGTCTCCTG CCGAAACTTC GAGTCACTTG TGATCCTGTT GGACTCCATG 
GATGATGTGG ATTCCATCTG CCGCGCCCGG AGGGTCCCCT GGCTGCCTCC CCGGTGCCCC 
CCGAGGATCC ACCTTATCCT CTCAGCCTGC TCGGGGAAGC CGGGGATTCT AGACATCATG 
AGACAGACGC TGACGGACCC AGAGGCCTAC TGGGAGGTGA AACCCCTCTC CGGAAACCAA 
GGGCAGGAGA TGATCCAGCT CCTGCTGGCC GCCTCGCGGA GGACGCTGAG CCCAGGGCAG 
AGGGAGCTGC TCTGGGCCAG CCTCCCAGAG TGTGGGCACC CAGGACGGCT GAGGCTGGCC 
TTTGAGGAGG CCCGGAAGTG GGCCTCCTTC ACCATACCTG CCCCTCTGGC CTCCACTGCT 
AAGGAAGCGA TCCATCAGCT GTGTGCCCGC CTGGAGCAGA CACACGGGCA GCTCCTGGTG 
GCCCACGTGC TGAGCTACAT CGTGTCCTCC CGGCATGGGC TCTCAGAGGC AGAGCTGAAG 
GACGTCCTGT CCTTGGACGA CGAGGTCCTG GAGGCGGTGT ACCAGGACTG GACCCCGCCC 
AGCAAGGAGC TGTTGCGCTT CCCCCCGTTG CTGTGGGTGA GGCTCCGTCG GGATCTCGGC 
CACTGCTTGG CCCGGCGGCC GGTGGACGGC TTCACGCTCC TGGCCATCGC CCACAGGCAG 
CTGACTGAGG TGATCCAAGA GCGCTACCTG TCTGGGCCCG AGAGAGCCAA GAGGCACGGA 
GTCTTGGCTG ACTTCTTCTC AGGGGCCTGG AGCCAGGGCA CAAAGAAACT CATCACCCTG 
CCACTCGTGG GGAAGCCCCT GAACCTGGAC CGCAAGGTGG CGCCTCAGCC CCTGTGGTTC 
TCAGACACAG TTGCAAATCT GCGGAAGCTG AAGGAGTTGC CCCATCACCT GGTCCACTCA 
GGCCGCTTCG AAGAGCTGAA GCAGGAGGTT CTGGGCAGCA TGAACTGGAT TTCCTGCCGG 
GGCGTCTCTG GGGGCATCGA GGGGCTGTTG GAGGACTTTG ACCTGTATGG CCCTCACCTG 
GATTGTCCCG AGGTCGGCCT GGTCCGGGAC GCCCTCCAGC TGTGCCGCCC GGCCCTGGAG 
CTCCGAGGCA TGGAGAGGAG CATCCTCTAC ACTGAACTCC TGGCCAGACT CCATTTCTTC 
GCCACCTCAC ATCCAGCGCT GGTGGGACAG CTGTGCCAAC AGGCCCAGAG CTGGTTCCGG 
CTGTGCCCCC ACCCTGTGCT GGTACCTCTC GGGGGGTTCC TCCAGCCCCC CGGGGGACCC 
CTCCGGGCAA CCTTCACTGG CTGTCACAAG GGCATCACTG CCATGGGCTG GAGCCTGGAA 
GAGAAGCTGC TGGTAGTTGG CACCCAGGAT GGCCTCATGG CTGTGTGGGA CATGGAAGAG 
CAGCAGGTGA TCCACATCCT AACTGGACAC ACAGGAGAGG TGAGGTCTGT GAAAGTGTTT 
GGCAAGGGGA CACTCGTCCT CTCTGCCTCA AAGGACCACA CACTGCGTTT GTGGAACTTA 
CTCTCTGGCC AGCAGAAATT CACCATTTGG GATGGAGGCT CAAAAGATCC CACTGACCCT 
GAGATCTGGA GTCTTCATGT GGATGAAGCA AAGAAGGTTG TGTATTTGGC ATCTGGCTCA 
AAGATCAGTG CTTGGAATCT GGAAACTGCA GAGCTGGTTT TCCATATCCT GGGAGATGCC 
TCCGACTGCT GGATGTGCAC AGCGGTGCTG GCTTCCCGGG CCACGCTGCT GACCGTGTCT 
GAGGGCGGTG TGGTCAGTCT GTGGAGCTCA GGCACAGGGA AACTGCAGGG GAGGCACCAT 
TTATCCAGCA TCAAAGAAGA AACACCTACC TGTGGGGTCT CGGTCCAGAA GCAAGGAAAG 
ATGGTGACTG GGTTCAGCAA AGGCTCCATC TCTTTGGTTT CCCCCGGAGG AGATAGCCTG 
CTGGAGAAGC TTCCAGAAGC CGTGAGGTTC CTGGTAGTCT CTGAAGATGA GTCCCTTCTC 
GCTGCAGGGT TTGGCAGATC CGTGCGGATA TTCTTGGCAG ACTCGCAGGG GTTTCATCGA 
TTTATGGCCA CCGACCTTGA ACATGAGGAC ACCGTGGAGA TAGCTGGTTT TGGTCCTGAG 
AACAATCTGA TTGTCACTGC GTCCCGCGAT GCACTCATCC AGGTGTGGAG CCTGTCGGAG 
CAGGGGACCC TGCTGGACAT CCTGGAAGGT GTCGGAGCCC CCGTGAGCCT GCTGGCCTGG 
GCTGGGGCCC TGGTGGCATC TGCTTCTCAG CAGTCCTCAT CTTTGAAAGT CTGGGATCTC 
ACTTACATTC AGAAGCCGAG GGCCTGCACC CCATTTCTGG ATCGTACTGG CCTCACTGCT 
GTGTCACACA ATGGAAGCTA CGTTTACTTC CCCAAAATCG GAGACAAGAA CAAAGTTACC 
ATTTGGGACT CTGCAGAAGT TCTGGAGGCT GGGAAGGCCA AGGCCGAGGC ACCAGCAGAC 
CGGTGTCTCG TGACAGCCGG CTCCCCGGTT GCAGATGGTC ACTTGCTCAT CACATCTTCG 
CAGGAGCACA GAGAAGGAAG CAAGCTCCCC TGCCACTTCC ATAAGGGCAC TAATCCCATT 
GTGGGGGCTC TGCCCTCACG ACCCAATCAC CTCCCAACAC CATCAGGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022262630.1
CDS	435..4505
Translation	MSVGPLSPGTRQRLLQGQADPLPAPLSRSVAIFISSTVSDMDAEREALQSTAFPEVQTFCQKNGLMFEVVDLRWGIRNTEATDHMTTELCLEELDRCRKTSIGPAFVALLGDQYGPCPVPTLIEEKEWEALRSQLASKPRDLELVARRFRRDENAVPPTYLLQALGTAETQGPEEATLTSVLRSGAQEARRLGLITQEQWHRYHWSVIEWEIDRGLLSLTDGDQGATVFLREIQDLNKHILEDCALKMVDRLADGCLDTDAQNLLSGLKGRIADTQPGVLKAHHLPWSRDLVNPKNKAHARYLKELGEQFVARANHQVLEHLREQEAGRQELAWLYQEIRHHLGLSTEATWTFCGRQELLAQLGQRLRQSDGHPHSPLVLFGPPGIGKTALMCKLAEQMPGLLGRKTVTVLRLLGMSQMSSDARGLLQSICFQVCLAYGLPLPPAQVLEAHARVVQFFHTLLHTVSCRNFESLVILLDSMDDVDSICRARRVPWLPPRCPPRIHLILSACSGKPGILDIMRQTLTDPEAYWEVKPLSGNQGQEMIQLLLAASRRTLSPGQRELLWASLPECGHPGRLRLAFEEARKWASFTIPAPLASTAKEAIHQLCARLEQTHGQLLVAHVLSYIVSSRHGLSEAELKDVLSLDDEVLEAVYQDWTPPSKELLRFPPLLWVRLRRDLGHCLARRPVDGFTLLAIAHRQLTEVIQERYLSGPERAKRHGVLADFFSGAWSQGTKKLITLPLVGKPLNLDRKVAPQPLWFSDTVANLRKLKELPHHLVHSGRFEELKQEVLGSMNWISCRGVSGGIEGLLEDFDLYGPHLDCPEVGLVRDALQLCRPALELRGMERSILYTELLARLHFFATSHPALVGQLCQQAQSWFRLCPHPVLVPLGGFLQPPGGPLRATFTGCHKGITAMGWSLEEKLLVVGTQDGLMAVWDMEEQQVIHILTGHTGEVRSVKVFGKGTLVLSASKDHTLRLWNLLSGQQKFTIWDGGSKDPTDPEIWSLHVDEAKKVVYLASGSKISAWNLETAELVFHILGDASDCWMCTAVLASRATLLTVSEGGVVSLWSSGTGKLQGRHHLSSIKEETPTCGVSVQKQGKMVTGFSKGSISLVSPGGDSLLEKLPEAVRFLVVSEDESLLAAGFGRSVRIFLADSQGFHRFMATDLEHEDTVEIAGFGPENNLIVTASRDALIQVWSLSEQGTLLDILEGVGAPVSLLAWAGALVASASQQSSSLKVWDLTYIQKPRACTPFLDRTGLTAVSHNGSYVYFPKIGDKNKVTIWDSAEVLEAGKAKAEAPADRCLVTAGSPVADGHLLITSSQEHREGSKLPCHFHKGTNPIVGALPSRPNHLPTPSG

Target ORF information:

RefSeq Version	XM_022406922.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris NACHT and WD repeat domain containing 1 (NWD1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022406922.1

ORF Insert Sequence:

ATGAGTGTGG GCCCCCTCAG CCCGGGCACC CGCCAGCGAC TTCTCCAGGG TCAGGCAGAC 
CCCCTCCCAG CGCCTCTGTC CAGAAGTGTG GCCATCTTCA TCAGCTCCAC GGTCTCGGAT 
ATGGATGCAG AGAGAGAAGC TCTGCAGAGC ACTGCCTTCC CCGAAGTGCA GACCTTCTGC 
CAAAAGAATG GCCTGATGTT CGAGGTTGTT GACCTGAGGT GGGGTATTCG GAACACTGAG 
GCCACTGACC ACATGACCAC AGAACTCTGC TTGGAGGAGC TCGACCGGTG TCGGAAAACA 
TCCATAGGGC CGGCTTTTGT TGCCCTCTTA GGTGACCAGT ACGGCCCCTG TCCAGTCCCT 
ACACTGATCG AGGAGAAGGA GTGGGAGGCC CTGAGATCCC AGCTGGCCTC CAAGCCACGT 
GACCTGGAGC TGGTGGCTCG ACGCTTCCGG AGGGATGAGA ACGCCGTGCC CCCCACCTAC 
CTGCTTCAGG CCCTAGGCAC TGCGGAGACC CAGGGGCCCG AAGAGGCCAC CCTGACCTCT 
GTGCTGCGCT CCGGAGCCCA GGAGGCCCGG AGGCTGGGCC TCATCACCCA GGAGCAGTGG 
CACCGCTACC ACTGGTCAGT CATTGAGTGG GAGATAGACC GGGGCCTGCT GAGCTTGACA 
GACGGAGACC AGGGGGCGAC TGTCTTCTTG AGAGAGATCC AAGACCTCAA CAAACACATC 
CTGGAAGACT GCGCCCTCAA GATGGTGGAC CGGCTCGCAG ATGGCTGCCT GGACACAGAT 
GCCCAGAACC TTCTCAGTGG CCTCAAGGGA CGCATTGCCG ACACACAACC TGGAGTCCTC 
AAGGCCCACC ACCTGCCATG GAGCCGAGAC CTGGTGAACC CCAAAAACAA GGCTCATGCT 
CGGTACCTGA AAGAGCTGGG GGAGCAGTTT GTGGCCAGGG CTAACCACCA GGTCCTTGAG 
CACCTCCGGG AGCAGGAGGC AGGCAGGCAG GAGTTGGCGT GGCTCTACCA GGAGATCCGC 
CACCACCTGG GGCTGAGCAC CGAGGCCACC TGGACCTTTT GTGGCCGCCA GGAGCTCCTG 
GCCCAGCTGG GGCAGCGGCT CCGGCAGAGC GATGGCCATC CGCACTCACC CCTGGTGCTT 
TTTGGACCTC CAGGCATCGG AAAGACAGCG CTGATGTGCA AGTTGGCAGA ACAAATGCCG 
GGACTGCTTG GCCGCAAGAC AGTGACCGTC CTGCGGCTTC TGGGGATGTC ACAGATGAGC 
TCCGACGCCC GAGGCCTGCT CCAGAGCATC TGCTTCCAGG TGTGCCTGGC CTACGGGCTG 
CCCCTGCCCC CTGCCCAGGT CCTGGAGGCC CATGCCAGGG TGGTCCAGTT TTTCCACACC 
CTCCTCCACA CTGTCTCCTG CCGAAACTTC GAGTCACTTG TGATCCTGTT GGACTCCATG 
GATGATGTGG ATTCCATCTG CCGCGCCCGG AGGGTCCCCT GGCTGCCTCC CCGGTGCCCC 
CCGAGGATCC ACCTTATCCT CTCAGCCTGC TCGGGGAAGC CGGGGATTCT AGACATCATG 
AGACAGACGC TGACGGACCC AGAGGCCTAC TGGGAGGTGA AACCCCTCTC CGGAAACCAA 
GGGCAGGAGA TGATCCAGCT CCTGCTGGCC GCCTCGCGGA GGACGCTGAG CCCAGGGCAG 
AGGGAGCTGC TCTGGGCCAG CCTCCCAGAG TGTGGGCACC CAGGACGGCT GAGGCTGGCC 
TTTGAGGAGG CCCGGAAGTG GGCCTCCTTC ACCATACCTG CCCCTCTGGC CTCCACTGCT 
AAGGAAGCGA TCCATCAGCT GTGTGCCCGC CTGGAGCAGA CACACGGGCA GCTCCTGGTG 
GCCCACGTGC TGAGCTACAT CGTGTCCTCC CGGCATGGGC TCTCAGAGGC AGAGCTGAAG 
GACGTCCTGT CCTTGGACGA CGAGGTCCTG GAGGCGGTGT ACCAGGACTG GACCCCGCCC 
AGCAAGGAGC TGTTGCGCTT CCCCCCGTTG CTGTGGGTGA GGCTCCGTCG GGATCTCGGC 
CACTGCTTGG CCCGGCGGCC GGTGGACGGC TTCACGCTCC TGGCCATCGC CCACAGGCAG 
CTGACTGAGG TGATCCAAGA GCGCTACCTG TCTGGGCCCG AGAGAGCCAA GAGGCACGGA 
GTCTTGGCTG ACTTCTTCTC AGGGGCCTGG AGCCAGGGCA CAAAGAAACT CATCACCCTG 
CCACTCGTGG GGAAGCCCCT GAACCTGGAC CGCAAGGTGG CGCCTCAGCC CCTGTGGTTC 
TCAGACACAG TTGCAAATCT GCGGAAGCTG AAGGAGTTGC CCCATCACCT GGTCCACTCA 
GGCCGCTTCG AAGAGCTGAA GCAGGAGGTT CTGGGCAGCA TGAACTGGAT TTCCTGCCGG 
GGCGTCTCTG GGGGCATCGA GGGGCTGTTG GAGGACTTTG ACCTGTATGG CCCTCACCTG 
GATTGTCCCG AGGTCGGCCT GGTCCGGGAC GCCCTCCAGC TGTGCCGCCC GGCCCTGGAG 
CTCCGAGGCA TGGAGAGGAG CATCCTCTAC ACTGAACTCC TGGCCAGACT CCATTTCTTC 
GCCACCTCAC ATCCAGCGCT GGTGGGACAG CTGTGCCAAC AGGCCCAGAG CTGGTTCCGG 
CTGTGCCCCC ACCCTGTGCT GGTACCTCTC GGGGGGTTCC TCCAGCCCCC CGGGGGACCC 
CTCCGGGCAA CCTTCACTGG CTGTCACAAG GGCATCACTG CCATGGGCTG GAGCCTGGAA 
GAGAAGCTGC TGGTAGTTGG CACCCAGGAT GGCCTCATGG CTGTGTGGGA CATGGAAGAG 
CAGCAGGTGA TCCACATCCT AACTGGACAC ACAGGAGAGG TGAGGTCTGT GAAAGTGTTT 
GGCAAGGGGA CACTCGTCCT CTCTGCCTCA AAGGACCACA CACTGCGTTT GTGGAACTTA 
CTCTCTGGCC AGCAGAAATT CACCATTTGG GATGGAGGCT CAAAAGATCC CACTGACCCT 
GAGATCTGGA GTCTTCATGT GGATGAAGCA AAGAAGGTTG TGTATTTGGC ATCTGGCTCA 
AAGATCAGTG CTTGGAATCT GGAAACTGCA GAGCTGGTTT TCCATATCCT GGGAGATGCC 
TCCGACTGCT GGATGTGCAC AGCGGTGCTG GCTTCCCGGG CCACGCTGCT GACCGTGTCT 
GAGGGCGGTG TGGTCAGTCT GTGGAGCTCA GGCACAGGGA AACTGCAGGG GAGGCACCAT 
TTATCCAGCA TCAAAGAAGA AACACCTACC TGTGGGGTCT CGGTCCAGAA GCAAGGAAAG 
ATGGTGACTG GGTTCAGCAA AGGCTCCATC TCTTTGGTTT CCCCCGGAGG AGATAGCCTG 
CTGGAGAAGC TTCCAGAAGC CGTGAGGTTC CTGGTAGTCT CTGAAGATGA GTCCCTTCTC 
GCTGCAGGGT TTGGCAGATC CGTGCGGATA TTCTTGGCAG ACTCGCAGGG GTTTCATCGA 
TTTATGGCCA CCGACCTTGA ACATGAGGAC ACCGTGGAGA TAGCTGGTTT TGGTCCTGAG 
AACAATCTGA TTGTCACTGC GTCCCGCGAT GCACTCATCC AGGTGTGGAG CCTGTCGGAG 
CAGGGGACCC TGCTGGACAT CCTGGAAGGT GTCGGAGCCC CCGTGAGCCT GCTGGCCTGG 
GCTGGGGCCC TGGTGGCATC TGCTTCTCAG CAGTCCTCAT CTTTGAAAGT CTGGGATCTC 
ACTTACATTC AGAAGCCGAG GGCCTGCACC CCATTTCTGG ATCGTACTGG CCTCACTGCT 
GTGTCACACA ATGGAAGCTA CGTTTACTTC CCCAAAATCG GAGACAAGAA CAAAGTTACC 
ATTTGGGACT CTGCAGAAGT TCTGGAGGCT GGGAAGGCCA AGGCCGAGGC ACCAGCAGAC 
CGGTGTCTCG TGACAGCCGG CTCCCCGGTT GCAGATGGTC ACTTGCTCAT CACATCTTCG 
CAGGAGCACA GAGAAGGAAG CAAGCTCCCC TGCCACTTCC ATAAGGGCAC TAATCCCATT 
GTGGGGGCTC TGCCCTCACG ACCCAATCAC CTCCCAACAC CATCAGGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NWD1 ( XM_014121994.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013977469.1 Canis lupus familiaris NACHT and WD repeat domain containing 1 (NWD1), transcript variant X3, mRNA.

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CloneID	OCe41722
Clone ID Related Accession (Same CDS sequence)	XM_014121994.1 , XM_022406921.1 , XM_022406920.1
Accession Version	XM_014121994.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	NACHT domain- and WD repeat-containing protein 1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726096.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACC GGCTCGCAGA TGGCTGCCTG GACACAGATG CCCAGAACCT TCTCAGTGGC 
CTCAAGGGAC GCATTGCCGA CACACAACCT GGAGTCCTCA AGGCCCACCA CCTGCCATGG 
AGCCGAGACC TGGTGAACCC CAAAAACAAG GCTCATGCTC GGTACCTGAA AGAGCTGGGG 
GAGCAGTTTG TGGCCAGGGC TAACCACCAG GTCCTTGAGC ACCTCCGGGA GCAGGAGGCA 
GGCAGGCAGG AGTTGGCGTG GCTCTACCAG GAGATCCGCC ACCACCTGGG GCTGAGCACC 
GAGGCCACCT GGACCTTTTG TGGCCGCCAG GAGCTCCTGG CCCAGCTGGG GCAGCGGCTC 
CGGCAGAGCG ATGGCCATCC GCACTCACCC CTGGTGCTTT TTGGACCTCC AGGCATCGGA 
AAGACAGCGC TGATGTGCAA GTTGGCAGAA CAAATGCCGG GACTGCTTGG CCGCAAGACA 
GTGACCGTCC TGCGGCTTCT GGGGATGTCA CAGATGAGCT CCGACGCCCG AGGCCTGCTC 
CAGAGCATCT GCTTCCAGGT GTGCCTGGCC TACGGGCTGC CCCTGCCCCC TGCCCAGGTC 
CTGGAGGCCC ATGCCAGGGT GGTCCAGTTT TTCCACACCC TCCTCCACAC TGTCTCCTGC 
CGAAACTTCG AGTCACTTGT GATCCTGTTG GACTCCATGG ATGATGTGGA TTCCATCTGC 
CGCGCCCGGA GGGTCCCCTG GCTGCCTCCC CGGTGCCCCC CGAGGATCCA CCTTATCCTC 
TCAGCCTGCT CGGGGAAGCC GGGGATTCTA GACATCATGA GACAGACGCT GACGGACCCA 
GAGGCCTACT GGGAGGTGAA ACCCCTCTCC GGAAACCAAG GGCAGGAGAT GATCCAGCTC 
CTGCTGGCCG CCTCGCGGAG GACGCTGAGC CCAGGGCAGA GGGAGCTGCT CTGGGCCAGC 
CTCCCAGAGT GTGGGCACCC AGGACGGCTG AGGCTGGCCT TTGAGGAGGC CCGGAAGTGG 
GCCTCCTTCA CCATACCTGC CCCTCTGGCC TCCACTGCTA AGGAAGCGAT CCATCAGCTG 
TGTGCCCGCC TGGAGCAGAC ACACGGGCAG CTCCTGGTGG CCCACGTGCT GAGCTACATC 
GTGTCCTCCC GGCATGGGCT CTCAGAGGCA GAGCTGAAGG ACGTCCTGTC CTTGGACGAC 
GAGGTCCTGG AGGCGGTGTA CCAGGACTGG ACCCCGCCCA GCAAGGAGCT GTTGCGCTTC 
CCCCCGTTGC TGTGGGTGAG GCTCCGTCGG GATCTCGGCC ACTGCTTGGC CCGGCGGCCG 
GTGGACGGCT TCACGCTCCT GGCCATCGCC CACAGGCAGC TGACTGAGGT GATCCAAGAG 
CGCTACCTGT CTGGGCCCGA GAGAGCCAAG AGGCACGGAG TCTTGGCTGA CTTCTTCTCA 
GGGGCCTGGA GCCAGGGCAC AAAGAAACTC ATCACCCTGC CACTCGTGGG GAAGCCCCTG 
AACCTGGACC GCAAGGTGGC GCCTCAGCCC CTGTGGTTCT CAGACACAGT TGCAAATCTG 
CGGAAGCTGA AGGAGTTGCC CCATCACCTG GTCCACTCAG GCCGCTTCGA AGAGCTGAAG 
CAGGAGGTTC TGGGCAGCAT GAACTGGATT TCCTGCCGGG GCGTCTCTGG GGGCATCGAG 
GGGCTGTTGG AGGACTTTGA CCTGTATGGC CCTCACCTGG ATTGTCCCGA GGTCGGCCTG 
GTCCGGGACG CCCTCCAGCT GTGCCGCCCG GCCCTGGAGC TCCGAGGCAT GGAGAGGAGC 
ATCCTCTACA CTGAACTCCT GGCCAGACTC CATTTCTTCG CCACCTCACA TCCAGCGCTG 
GTGGGACAGC TGTGCCAACA GGCCCAGAGC TGGTTCCGGC TGTGCCCCCA CCCTGTGCTG 
GTACCTCTCG GGGGGTTCCT CCAGCCCCCC GGGGGACCCC TCCGGGCAAC CTTCACTGGC 
TGTCACAAGG GCATCACTGC CATGGGCTGG AGCCTGGAAG AGAAGCTGCT GGTAGTTGGC 
ACCCAGGATG GCCTCATGGC TGTGTGGGAC ATGGAAGAGC AGCAGGTGAT CCACATCCTA 
ACTGGACACA CAGGAGAGGT GAGGTCTGTG AAAGTGTTTG GCAAGGGGAC ACTCGTCCTC 
TCTGCCTCAA AGGACCACAC ACTGCGTTTG TGGAACTTAC TCTCTGGCCA GCAGAAATTC 
ACCATTTGGG ATGGAGGCTC AAAAGATCCC ACTGACCCTG AGATCTGGAG TCTTCATGTG 
GATGAAGCAA AGAAGGTTGT GTATTTGGCA TCTGGCTCAA AGATCAGTGC TTGGAATCTG 
GAAACTGCAG AGCTGGTTTT CCATATCCTG GGAGATGCCT CCGACTGCTG GATGTGCACA 
GCGGTGCTGG CTTCCCGGGC CACGCTGCTG ACCGTGTCTG AGGGCGGTGT GGTCAGTCTG 
TGGAGCTCAG GCACAGGGAA ACTGCAGGGG AGGCACCATT TATCCAGCAT CAAAGAAGAA 
ACACCTACCT GTGGGGTCTC GGTCCAGAAG CAAGGAAAGA TGGTGACTGG GTTCAGCAAA 
GGCTCCATCT CTTTGGTTTC CCCCGGAGGA GATAGCCTGC TGGAGAAGCT TCCAGAAGCC 
GTGAGGTTCC TGGTAGTCTC TGAAGATGAG TCCCTTCTCG CTGCAGGGTT TGGCAGATCC 
GTGCGGATAT TCTTGGCAGA CTCGCAGGGG TTTCATCGAT TTATGGCCAC CGACCTTGAA 
CATGAGGACA CCGTGGAGAT AGCTGGTTTT GGTCCTGAGA ACAATCTGAT TGTCACTGCG 
TCCCGCGATG CACTCATCCA GGTGTGGAGC CTGTCGGAGC AGGGGACCCT GCTGGACATC 
CTGGAAGGTG TCGGAGCCCC CGTGAGCCTG CTGGCCTGGG CTGGGGCCCT GGTGGCATCT 
GCTTCTCAGC AGTCCTCATC TTTGAAAGTC TGGGATCTCA CTTACATTCA GAAGCCGAGG 
GCCTGCACCC CATTTCTGGA TCGTACTGGC CTCACTGCTG TGTCACACAA TGGAAGCTAC 
GTTTACTTCC CCAAAATCGG AGACAAGAAC AAAGTTACCA TTTGGGACTC TGCAGAAGGC 
GAGGAACAAG ATGCCCTGGA CACCTCCAAT GAGGTCAGGT GTCTGGAGGT TGCCCAGCAG 
GCCAATCTCC TTTTCACCGG CCTCGTTTCA GGGGTTGTCC TGGTGTTTCC CCTGAATTCC 
AGACAGGATG TGATGTGCAT CCCCCCTCCC GAGGCCCGGA AAGCCATCAA CTGCATGGCC 
CTCAGTAAAG GCGAGGAGCG CCTGGCCATC GCCTATGACA GCATTGTCCT TGTGCTGGAC 
ATCAGTCCCG GGGATCCCTG TCCGGTTATT GATGGGCCAA CATACACCTT CTATACCCAG 
CTGCCTGAGG CCATCTCCAG TGTGGCCGTT CTGGCTGACT ACCGCGTGAT CTACGGCATG 
ACCAACGGTG ACCTCTTTCT TTACGAGTGT GCGAATTCCA AAGTGTTCCC TCTGGAGGCC 
CACGGGAGCC AGGTCACCTG CATGCAGGTC AGCCACAAGG AGCAGCTGGC TGTCAGCGGG 
TCCGAGGAAG CCCTGCTGTG TCTCTGGGAC CTGCAGGCCT GCAAGTGGAG GTTCCAGATG 
AGCTACACGA ATTCTTACTG TAGAGGAGTC CAGTGTGTCT GCTTCTCCAA GGATGACAAG 
TATGTGTATG CGGGCTTGAA GGATCGCTCC ATCATTGTCT GGAGTGTGCT GGATGGCACC 
CTGCTGGCTG TCCAGTTTGT CCATGCTGTG GTGAACAGGA TCATCCCAAC TTCGAATGGC 
TTCATTGCCC CCACTAGACA TGGCTACCTG ATCCGCGAGC GTTTCCAGTG CCCTTCATCG 
AGAGTCTCAC AGCAAGATCC TCTCAAGAAC TTCAAGAAGG CAGTGTGGAA GGTCAAATCC 
AGGCAGAGAG AAGAGCTGGC AGCAGCTGCA GGAGCACTCC AGGACTCGAG ATCGGCAAGT 
GCCCAGGGAA ATGAACGTAA ATCGAACAAA CGCTCACAAG TCTGCCTGCT AGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013977469.1
CDS	211..4287
Translation	MVDRLADGCLDTDAQNLLSGLKGRIADTQPGVLKAHHLPWSRDLVNPKNKAHARYLKELGEQFVARANHQVLEHLREQEAGRQELAWLYQEIRHHLGLSTEATWTFCGRQELLAQLGQRLRQSDGHPHSPLVLFGPPGIGKTALMCKLAEQMPGLLGRKTVTVLRLLGMSQMSSDARGLLQSICFQVCLAYGLPLPPAQVLEAHARVVQFFHTLLHTVSCRNFESLVILLDSMDDVDSICRARRVPWLPPRCPPRIHLILSACSGKPGILDIMRQTLTDPEAYWEVKPLSGNQGQEMIQLLLAASRRTLSPGQRELLWASLPECGHPGRLRLAFEEARKWASFTIPAPLASTAKEAIHQLCARLEQTHGQLLVAHVLSYIVSSRHGLSEAELKDVLSLDDEVLEAVYQDWTPPSKELLRFPPLLWVRLRRDLGHCLARRPVDGFTLLAIAHRQLTEVIQERYLSGPERAKRHGVLADFFSGAWSQGTKKLITLPLVGKPLNLDRKVAPQPLWFSDTVANLRKLKELPHHLVHSGRFEELKQEVLGSMNWISCRGVSGGIEGLLEDFDLYGPHLDCPEVGLVRDALQLCRPALELRGMERSILYTELLARLHFFATSHPALVGQLCQQAQSWFRLCPHPVLVPLGGFLQPPGGPLRATFTGCHKGITAMGWSLEEKLLVVGTQDGLMAVWDMEEQQVIHILTGHTGEVRSVKVFGKGTLVLSASKDHTLRLWNLLSGQQKFTIWDGGSKDPTDPEIWSLHVDEAKKVVYLASGSKISAWNLETAELVFHILGDASDCWMCTAVLASRATLLTVSEGGVVSLWSSGTGKLQGRHHLSSIKEETPTCGVSVQKQGKMVTGFSKGSISLVSPGGDSLLEKLPEAVRFLVVSEDESLLAAGFGRSVRIFLADSQGFHRFMATDLEHEDTVEIAGFGPENNLIVTASRDALIQVWSLSEQGTLLDILEGVGAPVSLLAWAGALVASASQQSSSLKVWDLTYIQKPRACTPFLDRTGLTAVSHNGSYVYFPKIGDKNKVTIWDSAEGEEQDALDTSNEVRCLEVAQQANLLFTGLVSGVVLVFPLNSRQDVMCIPPPEARKAINCMALSKGEERLAIAYDSIVLVLDISPGDPCPVIDGPTYTFYTQLPEAISSVAVLADYRVIYGMTNGDLFLYECANSKVFPLEAHGSQVTCMQVSHKEQLAVSGSEEALLCLWDLQACKWRFQMSYTNSYCRGVQCVCFSKDDKYVYAGLKDRSIIVWSVLDGTLLAVQFVHAVVNRIIPTSNGFIAPTRHGYLIRERFQCPSSRVSQQDPLKNFKKAVWKVKSRQREELAAAAGALQDSRSASAQGNERKSNKRSQVCLLV

Target ORF information:

RefSeq Version	XM_014121994.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris NACHT and WD repeat domain containing 1 (NWD1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121994.1

ORF Insert Sequence:

ATGGTGGACC GGCTCGCAGA TGGCTGCCTG GACACAGATG CCCAGAACCT TCTCAGTGGC 
CTCAAGGGAC GCATTGCCGA CACACAACCT GGAGTCCTCA AGGCCCACCA CCTGCCATGG 
AGCCGAGACC TGGTGAACCC CAAAAACAAG GCTCATGCTC GGTACCTGAA AGAGCTGGGG 
GAGCAGTTTG TGGCCAGGGC TAACCACCAG GTCCTTGAGC ACCTCCGGGA GCAGGAGGCA 
GGCAGGCAGG AGTTGGCGTG GCTCTACCAG GAGATCCGCC ACCACCTGGG GCTGAGCACC 
GAGGCCACCT GGACCTTTTG TGGCCGCCAG GAGCTCCTGG CCCAGCTGGG GCAGCGGCTC 
CGGCAGAGCG ATGGCCATCC GCACTCACCC CTGGTGCTTT TTGGACCTCC AGGCATCGGA 
AAGACAGCGC TGATGTGCAA GTTGGCAGAA CAAATGCCGG GACTGCTTGG CCGCAAGACA 
GTGACCGTCC TGCGGCTTCT GGGGATGTCA CAGATGAGCT CCGACGCCCG AGGCCTGCTC 
CAGAGCATCT GCTTCCAGGT GTGCCTGGCC TACGGGCTGC CCCTGCCCCC TGCCCAGGTC 
CTGGAGGCCC ATGCCAGGGT GGTCCAGTTT TTCCACACCC TCCTCCACAC TGTCTCCTGC 
CGAAACTTCG AGTCACTTGT GATCCTGTTG GACTCCATGG ATGATGTGGA TTCCATCTGC 
CGCGCCCGGA GGGTCCCCTG GCTGCCTCCC CGGTGCCCCC CGAGGATCCA CCTTATCCTC 
TCAGCCTGCT CGGGGAAGCC GGGGATTCTA GACATCATGA GACAGACGCT GACGGACCCA 
GAGGCCTACT GGGAGGTGAA ACCCCTCTCC GGAAACCAAG GGCAGGAGAT GATCCAGCTC 
CTGCTGGCCG CCTCGCGGAG GACGCTGAGC CCAGGGCAGA GGGAGCTGCT CTGGGCCAGC 
CTCCCAGAGT GTGGGCACCC AGGACGGCTG AGGCTGGCCT TTGAGGAGGC CCGGAAGTGG 
GCCTCCTTCA CCATACCTGC CCCTCTGGCC TCCACTGCTA AGGAAGCGAT CCATCAGCTG 
TGTGCCCGCC TGGAGCAGAC ACACGGGCAG CTCCTGGTGG CCCACGTGCT GAGCTACATC 
GTGTCCTCCC GGCATGGGCT CTCAGAGGCA GAGCTGAAGG ACGTCCTGTC CTTGGACGAC 
GAGGTCCTGG AGGCGGTGTA CCAGGACTGG ACCCCGCCCA GCAAGGAGCT GTTGCGCTTC 
CCCCCGTTGC TGTGGGTGAG GCTCCGTCGG GATCTCGGCC ACTGCTTGGC CCGGCGGCCG 
GTGGACGGCT TCACGCTCCT GGCCATCGCC CACAGGCAGC TGACTGAGGT GATCCAAGAG 
CGCTACCTGT CTGGGCCCGA GAGAGCCAAG AGGCACGGAG TCTTGGCTGA CTTCTTCTCA 
GGGGCCTGGA GCCAGGGCAC AAAGAAACTC ATCACCCTGC CACTCGTGGG GAAGCCCCTG 
AACCTGGACC GCAAGGTGGC GCCTCAGCCC CTGTGGTTCT CAGACACAGT TGCAAATCTG 
CGGAAGCTGA AGGAGTTGCC CCATCACCTG GTCCACTCAG GCCGCTTCGA AGAGCTGAAG 
CAGGAGGTTC TGGGCAGCAT GAACTGGATT TCCTGCCGGG GCGTCTCTGG GGGCATCGAG 
GGGCTGTTGG AGGACTTTGA CCTGTATGGC CCTCACCTGG ATTGTCCCGA GGTCGGCCTG 
GTCCGGGACG CCCTCCAGCT GTGCCGCCCG GCCCTGGAGC TCCGAGGCAT GGAGAGGAGC 
ATCCTCTACA CTGAACTCCT GGCCAGACTC CATTTCTTCG CCACCTCACA TCCAGCGCTG 
GTGGGACAGC TGTGCCAACA GGCCCAGAGC TGGTTCCGGC TGTGCCCCCA CCCTGTGCTG 
GTACCTCTCG GGGGGTTCCT CCAGCCCCCC GGGGGACCCC TCCGGGCAAC CTTCACTGGC 
TGTCACAAGG GCATCACTGC CATGGGCTGG AGCCTGGAAG AGAAGCTGCT GGTAGTTGGC 
ACCCAGGATG GCCTCATGGC TGTGTGGGAC ATGGAAGAGC AGCAGGTGAT CCACATCCTA 
ACTGGACACA CAGGAGAGGT GAGGTCTGTG AAAGTGTTTG GCAAGGGGAC ACTCGTCCTC 
TCTGCCTCAA AGGACCACAC ACTGCGTTTG TGGAACTTAC TCTCTGGCCA GCAGAAATTC 
ACCATTTGGG ATGGAGGCTC AAAAGATCCC ACTGACCCTG AGATCTGGAG TCTTCATGTG 
GATGAAGCAA AGAAGGTTGT GTATTTGGCA TCTGGCTCAA AGATCAGTGC TTGGAATCTG 
GAAACTGCAG AGCTGGTTTT CCATATCCTG GGAGATGCCT CCGACTGCTG GATGTGCACA 
GCGGTGCTGG CTTCCCGGGC CACGCTGCTG ACCGTGTCTG AGGGCGGTGT GGTCAGTCTG 
TGGAGCTCAG GCACAGGGAA ACTGCAGGGG AGGCACCATT TATCCAGCAT CAAAGAAGAA 
ACACCTACCT GTGGGGTCTC GGTCCAGAAG CAAGGAAAGA TGGTGACTGG GTTCAGCAAA 
GGCTCCATCT CTTTGGTTTC CCCCGGAGGA GATAGCCTGC TGGAGAAGCT TCCAGAAGCC 
GTGAGGTTCC TGGTAGTCTC TGAAGATGAG TCCCTTCTCG CTGCAGGGTT TGGCAGATCC 
GTGCGGATAT TCTTGGCAGA CTCGCAGGGG TTTCATCGAT TTATGGCCAC CGACCTTGAA 
CATGAGGACA CCGTGGAGAT AGCTGGTTTT GGTCCTGAGA ACAATCTGAT TGTCACTGCG 
TCCCGCGATG CACTCATCCA GGTGTGGAGC CTGTCGGAGC AGGGGACCCT GCTGGACATC 
CTGGAAGGTG TCGGAGCCCC CGTGAGCCTG CTGGCCTGGG CTGGGGCCCT GGTGGCATCT 
GCTTCTCAGC AGTCCTCATC TTTGAAAGTC TGGGATCTCA CTTACATTCA GAAGCCGAGG 
GCCTGCACCC CATTTCTGGA TCGTACTGGC CTCACTGCTG TGTCACACAA TGGAAGCTAC 
GTTTACTTCC CCAAAATCGG AGACAAGAAC AAAGTTACCA TTTGGGACTC TGCAGAAGGC 
GAGGAACAAG ATGCCCTGGA CACCTCCAAT GAGGTCAGGT GTCTGGAGGT TGCCCAGCAG 
GCCAATCTCC TTTTCACCGG CCTCGTTTCA GGGGTTGTCC TGGTGTTTCC CCTGAATTCC 
AGACAGGATG TGATGTGCAT CCCCCCTCCC GAGGCCCGGA AAGCCATCAA CTGCATGGCC 
CTCAGTAAAG GCGAGGAGCG CCTGGCCATC GCCTATGACA GCATTGTCCT TGTGCTGGAC 
ATCAGTCCCG GGGATCCCTG TCCGGTTATT GATGGGCCAA CATACACCTT CTATACCCAG 
CTGCCTGAGG CCATCTCCAG TGTGGCCGTT CTGGCTGACT ACCGCGTGAT CTACGGCATG 
ACCAACGGTG ACCTCTTTCT TTACGAGTGT GCGAATTCCA AAGTGTTCCC TCTGGAGGCC 
CACGGGAGCC AGGTCACCTG CATGCAGGTC AGCCACAAGG AGCAGCTGGC TGTCAGCGGG 
TCCGAGGAAG CCCTGCTGTG TCTCTGGGAC CTGCAGGCCT GCAAGTGGAG GTTCCAGATG 
AGCTACACGA ATTCTTACTG TAGAGGAGTC CAGTGTGTCT GCTTCTCCAA GGATGACAAG 
TATGTGTATG CGGGCTTGAA GGATCGCTCC ATCATTGTCT GGAGTGTGCT GGATGGCACC 
CTGCTGGCTG TCCAGTTTGT CCATGCTGTG GTGAACAGGA TCATCCCAAC TTCGAATGGC 
TTCATTGCCC CCACTAGACA TGGCTACCTG ATCCGCGAGC GTTTCCAGTG CCCTTCATCG 
AGAGTCTCAC AGCAAGATCC TCTCAAGAAC TTCAAGAAGG CAGTGTGGAA GGTCAAATCC 
AGGCAGAGAG AAGAGCTGGC AGCAGCTGCA GGAGCACTCC AGGACTCGAG ATCGGCAAGT 
GCCCAGGGAA ATGAACGTAA ATCGAACAAA CGCTCACAAG TCTGCCTGCT AGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NWD1 ( XM_022406921.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022262629.1 Canis lupus familiaris NACHT and WD repeat domain containing 1 (NWD1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe41722
Clone ID Related Accession (Same CDS sequence)	XM_014121994.1 , XM_022406921.1 , XM_022406920.1
Accession Version	XM_022406921.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	NACHT domain- and WD repeat-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACC GGCTCGCAGA TGGCTGCCTG GACACAGATG CCCAGAACCT TCTCAGTGGC 
CTCAAGGGAC GCATTGCCGA CACACAACCT GGAGTCCTCA AGGCCCACCA CCTGCCATGG 
AGCCGAGACC TGGTGAACCC CAAAAACAAG GCTCATGCTC GGTACCTGAA AGAGCTGGGG 
GAGCAGTTTG TGGCCAGGGC TAACCACCAG GTCCTTGAGC ACCTCCGGGA GCAGGAGGCA 
GGCAGGCAGG AGTTGGCGTG GCTCTACCAG GAGATCCGCC ACCACCTGGG GCTGAGCACC 
GAGGCCACCT GGACCTTTTG TGGCCGCCAG GAGCTCCTGG CCCAGCTGGG GCAGCGGCTC 
CGGCAGAGCG ATGGCCATCC GCACTCACCC CTGGTGCTTT TTGGACCTCC AGGCATCGGA 
AAGACAGCGC TGATGTGCAA GTTGGCAGAA CAAATGCCGG GACTGCTTGG CCGCAAGACA 
GTGACCGTCC TGCGGCTTCT GGGGATGTCA CAGATGAGCT CCGACGCCCG AGGCCTGCTC 
CAGAGCATCT GCTTCCAGGT GTGCCTGGCC TACGGGCTGC CCCTGCCCCC TGCCCAGGTC 
CTGGAGGCCC ATGCCAGGGT GGTCCAGTTT TTCCACACCC TCCTCCACAC TGTCTCCTGC 
CGAAACTTCG AGTCACTTGT GATCCTGTTG GACTCCATGG ATGATGTGGA TTCCATCTGC 
CGCGCCCGGA GGGTCCCCTG GCTGCCTCCC CGGTGCCCCC CGAGGATCCA CCTTATCCTC 
TCAGCCTGCT CGGGGAAGCC GGGGATTCTA GACATCATGA GACAGACGCT GACGGACCCA 
GAGGCCTACT GGGAGGTGAA ACCCCTCTCC GGAAACCAAG GGCAGGAGAT GATCCAGCTC 
CTGCTGGCCG CCTCGCGGAG GACGCTGAGC CCAGGGCAGA GGGAGCTGCT CTGGGCCAGC 
CTCCCAGAGT GTGGGCACCC AGGACGGCTG AGGCTGGCCT TTGAGGAGGC CCGGAAGTGG 
GCCTCCTTCA CCATACCTGC CCCTCTGGCC TCCACTGCTA AGGAAGCGAT CCATCAGCTG 
TGTGCCCGCC TGGAGCAGAC ACACGGGCAG CTCCTGGTGG CCCACGTGCT GAGCTACATC 
GTGTCCTCCC GGCATGGGCT CTCAGAGGCA GAGCTGAAGG ACGTCCTGTC CTTGGACGAC 
GAGGTCCTGG AGGCGGTGTA CCAGGACTGG ACCCCGCCCA GCAAGGAGCT GTTGCGCTTC 
CCCCCGTTGC TGTGGGTGAG GCTCCGTCGG GATCTCGGCC ACTGCTTGGC CCGGCGGCCG 
GTGGACGGCT TCACGCTCCT GGCCATCGCC CACAGGCAGC TGACTGAGGT GATCCAAGAG 
CGCTACCTGT CTGGGCCCGA GAGAGCCAAG AGGCACGGAG TCTTGGCTGA CTTCTTCTCA 
GGGGCCTGGA GCCAGGGCAC AAAGAAACTC ATCACCCTGC CACTCGTGGG GAAGCCCCTG 
AACCTGGACC GCAAGGTGGC GCCTCAGCCC CTGTGGTTCT CAGACACAGT TGCAAATCTG 
CGGAAGCTGA AGGAGTTGCC CCATCACCTG GTCCACTCAG GCCGCTTCGA AGAGCTGAAG 
CAGGAGGTTC TGGGCAGCAT GAACTGGATT TCCTGCCGGG GCGTCTCTGG GGGCATCGAG 
GGGCTGTTGG AGGACTTTGA CCTGTATGGC CCTCACCTGG ATTGTCCCGA GGTCGGCCTG 
GTCCGGGACG CCCTCCAGCT GTGCCGCCCG GCCCTGGAGC TCCGAGGCAT GGAGAGGAGC 
ATCCTCTACA CTGAACTCCT GGCCAGACTC CATTTCTTCG CCACCTCACA TCCAGCGCTG 
GTGGGACAGC TGTGCCAACA GGCCCAGAGC TGGTTCCGGC TGTGCCCCCA CCCTGTGCTG 
GTACCTCTCG GGGGGTTCCT CCAGCCCCCC GGGGGACCCC TCCGGGCAAC CTTCACTGGC 
TGTCACAAGG GCATCACTGC CATGGGCTGG AGCCTGGAAG AGAAGCTGCT GGTAGTTGGC 
ACCCAGGATG GCCTCATGGC TGTGTGGGAC ATGGAAGAGC AGCAGGTGAT CCACATCCTA 
ACTGGACACA CAGGAGAGGT GAGGTCTGTG AAAGTGTTTG GCAAGGGGAC ACTCGTCCTC 
TCTGCCTCAA AGGACCACAC ACTGCGTTTG TGGAACTTAC TCTCTGGCCA GCAGAAATTC 
ACCATTTGGG ATGGAGGCTC AAAAGATCCC ACTGACCCTG AGATCTGGAG TCTTCATGTG 
GATGAAGCAA AGAAGGTTGT GTATTTGGCA TCTGGCTCAA AGATCAGTGC TTGGAATCTG 
GAAACTGCAG AGCTGGTTTT CCATATCCTG GGAGATGCCT CCGACTGCTG GATGTGCACA 
GCGGTGCTGG CTTCCCGGGC CACGCTGCTG ACCGTGTCTG AGGGCGGTGT GGTCAGTCTG 
TGGAGCTCAG GCACAGGGAA ACTGCAGGGG AGGCACCATT TATCCAGCAT CAAAGAAGAA 
ACACCTACCT GTGGGGTCTC GGTCCAGAAG CAAGGAAAGA TGGTGACTGG GTTCAGCAAA 
GGCTCCATCT CTTTGGTTTC CCCCGGAGGA GATAGCCTGC TGGAGAAGCT TCCAGAAGCC 
GTGAGGTTCC TGGTAGTCTC TGAAGATGAG TCCCTTCTCG CTGCAGGGTT TGGCAGATCC 
GTGCGGATAT TCTTGGCAGA CTCGCAGGGG TTTCATCGAT TTATGGCCAC CGACCTTGAA 
CATGAGGACA CCGTGGAGAT AGCTGGTTTT GGTCCTGAGA ACAATCTGAT TGTCACTGCG 
TCCCGCGATG CACTCATCCA GGTGTGGAGC CTGTCGGAGC AGGGGACCCT GCTGGACATC 
CTGGAAGGTG TCGGAGCCCC CGTGAGCCTG CTGGCCTGGG CTGGGGCCCT GGTGGCATCT 
GCTTCTCAGC AGTCCTCATC TTTGAAAGTC TGGGATCTCA CTTACATTCA GAAGCCGAGG 
GCCTGCACCC CATTTCTGGA TCGTACTGGC CTCACTGCTG TGTCACACAA TGGAAGCTAC 
GTTTACTTCC CCAAAATCGG AGACAAGAAC AAAGTTACCA TTTGGGACTC TGCAGAAGGC 
GAGGAACAAG ATGCCCTGGA CACCTCCAAT GAGGTCAGGT GTCTGGAGGT TGCCCAGCAG 
GCCAATCTCC TTTTCACCGG CCTCGTTTCA GGGGTTGTCC TGGTGTTTCC CCTGAATTCC 
AGACAGGATG TGATGTGCAT CCCCCCTCCC GAGGCCCGGA AAGCCATCAA CTGCATGGCC 
CTCAGTAAAG GCGAGGAGCG CCTGGCCATC GCCTATGACA GCATTGTCCT TGTGCTGGAC 
ATCAGTCCCG GGGATCCCTG TCCGGTTATT GATGGGCCAA CATACACCTT CTATACCCAG 
CTGCCTGAGG CCATCTCCAG TGTGGCCGTT CTGGCTGACT ACCGCGTGAT CTACGGCATG 
ACCAACGGTG ACCTCTTTCT TTACGAGTGT GCGAATTCCA AAGTGTTCCC TCTGGAGGCC 
CACGGGAGCC AGGTCACCTG CATGCAGGTC AGCCACAAGG AGCAGCTGGC TGTCAGCGGG 
TCCGAGGAAG CCCTGCTGTG TCTCTGGGAC CTGCAGGCCT GCAAGTGGAG GTTCCAGATG 
AGCTACACGA ATTCTTACTG TAGAGGAGTC CAGTGTGTCT GCTTCTCCAA GGATGACAAG 
TATGTGTATG CGGGCTTGAA GGATCGCTCC ATCATTGTCT GGAGTGTGCT GGATGGCACC 
CTGCTGGCTG TCCAGTTTGT CCATGCTGTG GTGAACAGGA TCATCCCAAC TTCGAATGGC 
TTCATTGCCC CCACTAGACA TGGCTACCTG ATCCGCGAGC GTTTCCAGTG CCCTTCATCG 
AGAGTCTCAC AGCAAGATCC TCTCAAGAAC TTCAAGAAGG CAGTGTGGAA GGTCAAATCC 
AGGCAGAGAG AAGAGCTGGC AGCAGCTGCA GGAGCACTCC AGGACTCGAG ATCGGCAAGT 
GCCCAGGGAA ATGAACGTAA ATCGAACAAA CGCTCACAAG TCTGCCTGCT AGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022262629.1
CDS	531..4607
Translation	MVDRLADGCLDTDAQNLLSGLKGRIADTQPGVLKAHHLPWSRDLVNPKNKAHARYLKELGEQFVARANHQVLEHLREQEAGRQELAWLYQEIRHHLGLSTEATWTFCGRQELLAQLGQRLRQSDGHPHSPLVLFGPPGIGKTALMCKLAEQMPGLLGRKTVTVLRLLGMSQMSSDARGLLQSICFQVCLAYGLPLPPAQVLEAHARVVQFFHTLLHTVSCRNFESLVILLDSMDDVDSICRARRVPWLPPRCPPRIHLILSACSGKPGILDIMRQTLTDPEAYWEVKPLSGNQGQEMIQLLLAASRRTLSPGQRELLWASLPECGHPGRLRLAFEEARKWASFTIPAPLASTAKEAIHQLCARLEQTHGQLLVAHVLSYIVSSRHGLSEAELKDVLSLDDEVLEAVYQDWTPPSKELLRFPPLLWVRLRRDLGHCLARRPVDGFTLLAIAHRQLTEVIQERYLSGPERAKRHGVLADFFSGAWSQGTKKLITLPLVGKPLNLDRKVAPQPLWFSDTVANLRKLKELPHHLVHSGRFEELKQEVLGSMNWISCRGVSGGIEGLLEDFDLYGPHLDCPEVGLVRDALQLCRPALELRGMERSILYTELLARLHFFATSHPALVGQLCQQAQSWFRLCPHPVLVPLGGFLQPPGGPLRATFTGCHKGITAMGWSLEEKLLVVGTQDGLMAVWDMEEQQVIHILTGHTGEVRSVKVFGKGTLVLSASKDHTLRLWNLLSGQQKFTIWDGGSKDPTDPEIWSLHVDEAKKVVYLASGSKISAWNLETAELVFHILGDASDCWMCTAVLASRATLLTVSEGGVVSLWSSGTGKLQGRHHLSSIKEETPTCGVSVQKQGKMVTGFSKGSISLVSPGGDSLLEKLPEAVRFLVVSEDESLLAAGFGRSVRIFLADSQGFHRFMATDLEHEDTVEIAGFGPENNLIVTASRDALIQVWSLSEQGTLLDILEGVGAPVSLLAWAGALVASASQQSSSLKVWDLTYIQKPRACTPFLDRTGLTAVSHNGSYVYFPKIGDKNKVTIWDSAEGEEQDALDTSNEVRCLEVAQQANLLFTGLVSGVVLVFPLNSRQDVMCIPPPEARKAINCMALSKGEERLAIAYDSIVLVLDISPGDPCPVIDGPTYTFYTQLPEAISSVAVLADYRVIYGMTNGDLFLYECANSKVFPLEAHGSQVTCMQVSHKEQLAVSGSEEALLCLWDLQACKWRFQMSYTNSYCRGVQCVCFSKDDKYVYAGLKDRSIIVWSVLDGTLLAVQFVHAVVNRIIPTSNGFIAPTRHGYLIRERFQCPSSRVSQQDPLKNFKKAVWKVKSRQREELAAAAGALQDSRSASAQGNERKSNKRSQVCLLV

Target ORF information:

RefSeq Version	XM_022406921.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris NACHT and WD repeat domain containing 1 (NWD1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022406921.1

ORF Insert Sequence:

ATGGTGGACC GGCTCGCAGA TGGCTGCCTG GACACAGATG CCCAGAACCT TCTCAGTGGC 
CTCAAGGGAC GCATTGCCGA CACACAACCT GGAGTCCTCA AGGCCCACCA CCTGCCATGG 
AGCCGAGACC TGGTGAACCC CAAAAACAAG GCTCATGCTC GGTACCTGAA AGAGCTGGGG 
GAGCAGTTTG TGGCCAGGGC TAACCACCAG GTCCTTGAGC ACCTCCGGGA GCAGGAGGCA 
GGCAGGCAGG AGTTGGCGTG GCTCTACCAG GAGATCCGCC ACCACCTGGG GCTGAGCACC 
GAGGCCACCT GGACCTTTTG TGGCCGCCAG GAGCTCCTGG CCCAGCTGGG GCAGCGGCTC 
CGGCAGAGCG ATGGCCATCC GCACTCACCC CTGGTGCTTT TTGGACCTCC AGGCATCGGA 
AAGACAGCGC TGATGTGCAA GTTGGCAGAA CAAATGCCGG GACTGCTTGG CCGCAAGACA 
GTGACCGTCC TGCGGCTTCT GGGGATGTCA CAGATGAGCT CCGACGCCCG AGGCCTGCTC 
CAGAGCATCT GCTTCCAGGT GTGCCTGGCC TACGGGCTGC CCCTGCCCCC TGCCCAGGTC 
CTGGAGGCCC ATGCCAGGGT GGTCCAGTTT TTCCACACCC TCCTCCACAC TGTCTCCTGC 
CGAAACTTCG AGTCACTTGT GATCCTGTTG GACTCCATGG ATGATGTGGA TTCCATCTGC 
CGCGCCCGGA GGGTCCCCTG GCTGCCTCCC CGGTGCCCCC CGAGGATCCA CCTTATCCTC 
TCAGCCTGCT CGGGGAAGCC GGGGATTCTA GACATCATGA GACAGACGCT GACGGACCCA 
GAGGCCTACT GGGAGGTGAA ACCCCTCTCC GGAAACCAAG GGCAGGAGAT GATCCAGCTC 
CTGCTGGCCG CCTCGCGGAG GACGCTGAGC CCAGGGCAGA GGGAGCTGCT CTGGGCCAGC 
CTCCCAGAGT GTGGGCACCC AGGACGGCTG AGGCTGGCCT TTGAGGAGGC CCGGAAGTGG 
GCCTCCTTCA CCATACCTGC CCCTCTGGCC TCCACTGCTA AGGAAGCGAT CCATCAGCTG 
TGTGCCCGCC TGGAGCAGAC ACACGGGCAG CTCCTGGTGG CCCACGTGCT GAGCTACATC 
GTGTCCTCCC GGCATGGGCT CTCAGAGGCA GAGCTGAAGG ACGTCCTGTC CTTGGACGAC 
GAGGTCCTGG AGGCGGTGTA CCAGGACTGG ACCCCGCCCA GCAAGGAGCT GTTGCGCTTC 
CCCCCGTTGC TGTGGGTGAG GCTCCGTCGG GATCTCGGCC ACTGCTTGGC CCGGCGGCCG 
GTGGACGGCT TCACGCTCCT GGCCATCGCC CACAGGCAGC TGACTGAGGT GATCCAAGAG 
CGCTACCTGT CTGGGCCCGA GAGAGCCAAG AGGCACGGAG TCTTGGCTGA CTTCTTCTCA 
GGGGCCTGGA GCCAGGGCAC AAAGAAACTC ATCACCCTGC CACTCGTGGG GAAGCCCCTG 
AACCTGGACC GCAAGGTGGC GCCTCAGCCC CTGTGGTTCT CAGACACAGT TGCAAATCTG 
CGGAAGCTGA AGGAGTTGCC CCATCACCTG GTCCACTCAG GCCGCTTCGA AGAGCTGAAG 
CAGGAGGTTC TGGGCAGCAT GAACTGGATT TCCTGCCGGG GCGTCTCTGG GGGCATCGAG 
GGGCTGTTGG AGGACTTTGA CCTGTATGGC CCTCACCTGG ATTGTCCCGA GGTCGGCCTG 
GTCCGGGACG CCCTCCAGCT GTGCCGCCCG GCCCTGGAGC TCCGAGGCAT GGAGAGGAGC 
ATCCTCTACA CTGAACTCCT GGCCAGACTC CATTTCTTCG CCACCTCACA TCCAGCGCTG 
GTGGGACAGC TGTGCCAACA GGCCCAGAGC TGGTTCCGGC TGTGCCCCCA CCCTGTGCTG 
GTACCTCTCG GGGGGTTCCT CCAGCCCCCC GGGGGACCCC TCCGGGCAAC CTTCACTGGC 
TGTCACAAGG GCATCACTGC CATGGGCTGG AGCCTGGAAG AGAAGCTGCT GGTAGTTGGC 
ACCCAGGATG GCCTCATGGC TGTGTGGGAC ATGGAAGAGC AGCAGGTGAT CCACATCCTA 
ACTGGACACA CAGGAGAGGT GAGGTCTGTG AAAGTGTTTG GCAAGGGGAC ACTCGTCCTC 
TCTGCCTCAA AGGACCACAC ACTGCGTTTG TGGAACTTAC TCTCTGGCCA GCAGAAATTC 
ACCATTTGGG ATGGAGGCTC AAAAGATCCC ACTGACCCTG AGATCTGGAG TCTTCATGTG 
GATGAAGCAA AGAAGGTTGT GTATTTGGCA TCTGGCTCAA AGATCAGTGC TTGGAATCTG 
GAAACTGCAG AGCTGGTTTT CCATATCCTG GGAGATGCCT CCGACTGCTG GATGTGCACA 
GCGGTGCTGG CTTCCCGGGC CACGCTGCTG ACCGTGTCTG AGGGCGGTGT GGTCAGTCTG 
TGGAGCTCAG GCACAGGGAA ACTGCAGGGG AGGCACCATT TATCCAGCAT CAAAGAAGAA 
ACACCTACCT GTGGGGTCTC GGTCCAGAAG CAAGGAAAGA TGGTGACTGG GTTCAGCAAA 
GGCTCCATCT CTTTGGTTTC CCCCGGAGGA GATAGCCTGC TGGAGAAGCT TCCAGAAGCC 
GTGAGGTTCC TGGTAGTCTC TGAAGATGAG TCCCTTCTCG CTGCAGGGTT TGGCAGATCC 
GTGCGGATAT TCTTGGCAGA CTCGCAGGGG TTTCATCGAT TTATGGCCAC CGACCTTGAA 
CATGAGGACA CCGTGGAGAT AGCTGGTTTT GGTCCTGAGA ACAATCTGAT TGTCACTGCG 
TCCCGCGATG CACTCATCCA GGTGTGGAGC CTGTCGGAGC AGGGGACCCT GCTGGACATC 
CTGGAAGGTG TCGGAGCCCC CGTGAGCCTG CTGGCCTGGG CTGGGGCCCT GGTGGCATCT 
GCTTCTCAGC AGTCCTCATC TTTGAAAGTC TGGGATCTCA CTTACATTCA GAAGCCGAGG 
GCCTGCACCC CATTTCTGGA TCGTACTGGC CTCACTGCTG TGTCACACAA TGGAAGCTAC 
GTTTACTTCC CCAAAATCGG AGACAAGAAC AAAGTTACCA TTTGGGACTC TGCAGAAGGC 
GAGGAACAAG ATGCCCTGGA CACCTCCAAT GAGGTCAGGT GTCTGGAGGT TGCCCAGCAG 
GCCAATCTCC TTTTCACCGG CCTCGTTTCA GGGGTTGTCC TGGTGTTTCC CCTGAATTCC 
AGACAGGATG TGATGTGCAT CCCCCCTCCC GAGGCCCGGA AAGCCATCAA CTGCATGGCC 
CTCAGTAAAG GCGAGGAGCG CCTGGCCATC GCCTATGACA GCATTGTCCT TGTGCTGGAC 
ATCAGTCCCG GGGATCCCTG TCCGGTTATT GATGGGCCAA CATACACCTT CTATACCCAG 
CTGCCTGAGG CCATCTCCAG TGTGGCCGTT CTGGCTGACT ACCGCGTGAT CTACGGCATG 
ACCAACGGTG ACCTCTTTCT TTACGAGTGT GCGAATTCCA AAGTGTTCCC TCTGGAGGCC 
CACGGGAGCC AGGTCACCTG CATGCAGGTC AGCCACAAGG AGCAGCTGGC TGTCAGCGGG 
TCCGAGGAAG CCCTGCTGTG TCTCTGGGAC CTGCAGGCCT GCAAGTGGAG GTTCCAGATG 
AGCTACACGA ATTCTTACTG TAGAGGAGTC CAGTGTGTCT GCTTCTCCAA GGATGACAAG 
TATGTGTATG CGGGCTTGAA GGATCGCTCC ATCATTGTCT GGAGTGTGCT GGATGGCACC 
CTGCTGGCTG TCCAGTTTGT CCATGCTGTG GTGAACAGGA TCATCCCAAC TTCGAATGGC 
TTCATTGCCC CCACTAGACA TGGCTACCTG ATCCGCGAGC GTTTCCAGTG CCCTTCATCG 
AGAGTCTCAC AGCAAGATCC TCTCAAGAAC TTCAAGAAGG CAGTGTGGAA GGTCAAATCC 
AGGCAGAGAG AAGAGCTGGC AGCAGCTGCA GGAGCACTCC AGGACTCGAG ATCGGCAAGT 
GCCCAGGGAA ATGAACGTAA ATCGAACAAA CGCTCACAAG TCTGCCTGCT AGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NWD1 ( XM_022406920.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022262628.1 Canis lupus familiaris NACHT and WD repeat domain containing 1 (NWD1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe41722
Clone ID Related Accession (Same CDS sequence)	XM_014121994.1 , XM_022406921.1 , XM_022406920.1
Accession Version	XM_022406920.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	NACHT domain- and WD repeat-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACC GGCTCGCAGA TGGCTGCCTG GACACAGATG CCCAGAACCT TCTCAGTGGC 
CTCAAGGGAC GCATTGCCGA CACACAACCT GGAGTCCTCA AGGCCCACCA CCTGCCATGG 
AGCCGAGACC TGGTGAACCC CAAAAACAAG GCTCATGCTC GGTACCTGAA AGAGCTGGGG 
GAGCAGTTTG TGGCCAGGGC TAACCACCAG GTCCTTGAGC ACCTCCGGGA GCAGGAGGCA 
GGCAGGCAGG AGTTGGCGTG GCTCTACCAG GAGATCCGCC ACCACCTGGG GCTGAGCACC 
GAGGCCACCT GGACCTTTTG TGGCCGCCAG GAGCTCCTGG CCCAGCTGGG GCAGCGGCTC 
CGGCAGAGCG ATGGCCATCC GCACTCACCC CTGGTGCTTT TTGGACCTCC AGGCATCGGA 
AAGACAGCGC TGATGTGCAA GTTGGCAGAA CAAATGCCGG GACTGCTTGG CCGCAAGACA 
GTGACCGTCC TGCGGCTTCT GGGGATGTCA CAGATGAGCT CCGACGCCCG AGGCCTGCTC 
CAGAGCATCT GCTTCCAGGT GTGCCTGGCC TACGGGCTGC CCCTGCCCCC TGCCCAGGTC 
CTGGAGGCCC ATGCCAGGGT GGTCCAGTTT TTCCACACCC TCCTCCACAC TGTCTCCTGC 
CGAAACTTCG AGTCACTTGT GATCCTGTTG GACTCCATGG ATGATGTGGA TTCCATCTGC 
CGCGCCCGGA GGGTCCCCTG GCTGCCTCCC CGGTGCCCCC CGAGGATCCA CCTTATCCTC 
TCAGCCTGCT CGGGGAAGCC GGGGATTCTA GACATCATGA GACAGACGCT GACGGACCCA 
GAGGCCTACT GGGAGGTGAA ACCCCTCTCC GGAAACCAAG GGCAGGAGAT GATCCAGCTC 
CTGCTGGCCG CCTCGCGGAG GACGCTGAGC CCAGGGCAGA GGGAGCTGCT CTGGGCCAGC 
CTCCCAGAGT GTGGGCACCC AGGACGGCTG AGGCTGGCCT TTGAGGAGGC CCGGAAGTGG 
GCCTCCTTCA CCATACCTGC CCCTCTGGCC TCCACTGCTA AGGAAGCGAT CCATCAGCTG 
TGTGCCCGCC TGGAGCAGAC ACACGGGCAG CTCCTGGTGG CCCACGTGCT GAGCTACATC 
GTGTCCTCCC GGCATGGGCT CTCAGAGGCA GAGCTGAAGG ACGTCCTGTC CTTGGACGAC 
GAGGTCCTGG AGGCGGTGTA CCAGGACTGG ACCCCGCCCA GCAAGGAGCT GTTGCGCTTC 
CCCCCGTTGC TGTGGGTGAG GCTCCGTCGG GATCTCGGCC ACTGCTTGGC CCGGCGGCCG 
GTGGACGGCT TCACGCTCCT GGCCATCGCC CACAGGCAGC TGACTGAGGT GATCCAAGAG 
CGCTACCTGT CTGGGCCCGA GAGAGCCAAG AGGCACGGAG TCTTGGCTGA CTTCTTCTCA 
GGGGCCTGGA GCCAGGGCAC AAAGAAACTC ATCACCCTGC CACTCGTGGG GAAGCCCCTG 
AACCTGGACC GCAAGGTGGC GCCTCAGCCC CTGTGGTTCT CAGACACAGT TGCAAATCTG 
CGGAAGCTGA AGGAGTTGCC CCATCACCTG GTCCACTCAG GCCGCTTCGA AGAGCTGAAG 
CAGGAGGTTC TGGGCAGCAT GAACTGGATT TCCTGCCGGG GCGTCTCTGG GGGCATCGAG 
GGGCTGTTGG AGGACTTTGA CCTGTATGGC CCTCACCTGG ATTGTCCCGA GGTCGGCCTG 
GTCCGGGACG CCCTCCAGCT GTGCCGCCCG GCCCTGGAGC TCCGAGGCAT GGAGAGGAGC 
ATCCTCTACA CTGAACTCCT GGCCAGACTC CATTTCTTCG CCACCTCACA TCCAGCGCTG 
GTGGGACAGC TGTGCCAACA GGCCCAGAGC TGGTTCCGGC TGTGCCCCCA CCCTGTGCTG 
GTACCTCTCG GGGGGTTCCT CCAGCCCCCC GGGGGACCCC TCCGGGCAAC CTTCACTGGC 
TGTCACAAGG GCATCACTGC CATGGGCTGG AGCCTGGAAG AGAAGCTGCT GGTAGTTGGC 
ACCCAGGATG GCCTCATGGC TGTGTGGGAC ATGGAAGAGC AGCAGGTGAT CCACATCCTA 
ACTGGACACA CAGGAGAGGT GAGGTCTGTG AAAGTGTTTG GCAAGGGGAC ACTCGTCCTC 
TCTGCCTCAA AGGACCACAC ACTGCGTTTG TGGAACTTAC TCTCTGGCCA GCAGAAATTC 
ACCATTTGGG ATGGAGGCTC AAAAGATCCC ACTGACCCTG AGATCTGGAG TCTTCATGTG 
GATGAAGCAA AGAAGGTTGT GTATTTGGCA TCTGGCTCAA AGATCAGTGC TTGGAATCTG 
GAAACTGCAG AGCTGGTTTT CCATATCCTG GGAGATGCCT CCGACTGCTG GATGTGCACA 
GCGGTGCTGG CTTCCCGGGC CACGCTGCTG ACCGTGTCTG AGGGCGGTGT GGTCAGTCTG 
TGGAGCTCAG GCACAGGGAA ACTGCAGGGG AGGCACCATT TATCCAGCAT CAAAGAAGAA 
ACACCTACCT GTGGGGTCTC GGTCCAGAAG CAAGGAAAGA TGGTGACTGG GTTCAGCAAA 
GGCTCCATCT CTTTGGTTTC CCCCGGAGGA GATAGCCTGC TGGAGAAGCT TCCAGAAGCC 
GTGAGGTTCC TGGTAGTCTC TGAAGATGAG TCCCTTCTCG CTGCAGGGTT TGGCAGATCC 
GTGCGGATAT TCTTGGCAGA CTCGCAGGGG TTTCATCGAT TTATGGCCAC CGACCTTGAA 
CATGAGGACA CCGTGGAGAT AGCTGGTTTT GGTCCTGAGA ACAATCTGAT TGTCACTGCG 
TCCCGCGATG CACTCATCCA GGTGTGGAGC CTGTCGGAGC AGGGGACCCT GCTGGACATC 
CTGGAAGGTG TCGGAGCCCC CGTGAGCCTG CTGGCCTGGG CTGGGGCCCT GGTGGCATCT 
GCTTCTCAGC AGTCCTCATC TTTGAAAGTC TGGGATCTCA CTTACATTCA GAAGCCGAGG 
GCCTGCACCC CATTTCTGGA TCGTACTGGC CTCACTGCTG TGTCACACAA TGGAAGCTAC 
GTTTACTTCC CCAAAATCGG AGACAAGAAC AAAGTTACCA TTTGGGACTC TGCAGAAGGC 
GAGGAACAAG ATGCCCTGGA CACCTCCAAT GAGGTCAGGT GTCTGGAGGT TGCCCAGCAG 
GCCAATCTCC TTTTCACCGG CCTCGTTTCA GGGGTTGTCC TGGTGTTTCC CCTGAATTCC 
AGACAGGATG TGATGTGCAT CCCCCCTCCC GAGGCCCGGA AAGCCATCAA CTGCATGGCC 
CTCAGTAAAG GCGAGGAGCG CCTGGCCATC GCCTATGACA GCATTGTCCT TGTGCTGGAC 
ATCAGTCCCG GGGATCCCTG TCCGGTTATT GATGGGCCAA CATACACCTT CTATACCCAG 
CTGCCTGAGG CCATCTCCAG TGTGGCCGTT CTGGCTGACT ACCGCGTGAT CTACGGCATG 
ACCAACGGTG ACCTCTTTCT TTACGAGTGT GCGAATTCCA AAGTGTTCCC TCTGGAGGCC 
CACGGGAGCC AGGTCACCTG CATGCAGGTC AGCCACAAGG AGCAGCTGGC TGTCAGCGGG 
TCCGAGGAAG CCCTGCTGTG TCTCTGGGAC CTGCAGGCCT GCAAGTGGAG GTTCCAGATG 
AGCTACACGA ATTCTTACTG TAGAGGAGTC CAGTGTGTCT GCTTCTCCAA GGATGACAAG 
TATGTGTATG CGGGCTTGAA GGATCGCTCC ATCATTGTCT GGAGTGTGCT GGATGGCACC 
CTGCTGGCTG TCCAGTTTGT CCATGCTGTG GTGAACAGGA TCATCCCAAC TTCGAATGGC 
TTCATTGCCC CCACTAGACA TGGCTACCTG ATCCGCGAGC GTTTCCAGTG CCCTTCATCG 
AGAGTCTCAC AGCAAGATCC TCTCAAGAAC TTCAAGAAGG CAGTGTGGAA GGTCAAATCC 
AGGCAGAGAG AAGAGCTGGC AGCAGCTGCA GGAGCACTCC AGGACTCGAG ATCGGCAAGT 
GCCCAGGGAA ATGAACGTAA ATCGAACAAA CGCTCACAAG TCTGCCTGCT AGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022262628.1
CDS	260..4336
Translation	MVDRLADGCLDTDAQNLLSGLKGRIADTQPGVLKAHHLPWSRDLVNPKNKAHARYLKELGEQFVARANHQVLEHLREQEAGRQELAWLYQEIRHHLGLSTEATWTFCGRQELLAQLGQRLRQSDGHPHSPLVLFGPPGIGKTALMCKLAEQMPGLLGRKTVTVLRLLGMSQMSSDARGLLQSICFQVCLAYGLPLPPAQVLEAHARVVQFFHTLLHTVSCRNFESLVILLDSMDDVDSICRARRVPWLPPRCPPRIHLILSACSGKPGILDIMRQTLTDPEAYWEVKPLSGNQGQEMIQLLLAASRRTLSPGQRELLWASLPECGHPGRLRLAFEEARKWASFTIPAPLASTAKEAIHQLCARLEQTHGQLLVAHVLSYIVSSRHGLSEAELKDVLSLDDEVLEAVYQDWTPPSKELLRFPPLLWVRLRRDLGHCLARRPVDGFTLLAIAHRQLTEVIQERYLSGPERAKRHGVLADFFSGAWSQGTKKLITLPLVGKPLNLDRKVAPQPLWFSDTVANLRKLKELPHHLVHSGRFEELKQEVLGSMNWISCRGVSGGIEGLLEDFDLYGPHLDCPEVGLVRDALQLCRPALELRGMERSILYTELLARLHFFATSHPALVGQLCQQAQSWFRLCPHPVLVPLGGFLQPPGGPLRATFTGCHKGITAMGWSLEEKLLVVGTQDGLMAVWDMEEQQVIHILTGHTGEVRSVKVFGKGTLVLSASKDHTLRLWNLLSGQQKFTIWDGGSKDPTDPEIWSLHVDEAKKVVYLASGSKISAWNLETAELVFHILGDASDCWMCTAVLASRATLLTVSEGGVVSLWSSGTGKLQGRHHLSSIKEETPTCGVSVQKQGKMVTGFSKGSISLVSPGGDSLLEKLPEAVRFLVVSEDESLLAAGFGRSVRIFLADSQGFHRFMATDLEHEDTVEIAGFGPENNLIVTASRDALIQVWSLSEQGTLLDILEGVGAPVSLLAWAGALVASASQQSSSLKVWDLTYIQKPRACTPFLDRTGLTAVSHNGSYVYFPKIGDKNKVTIWDSAEGEEQDALDTSNEVRCLEVAQQANLLFTGLVSGVVLVFPLNSRQDVMCIPPPEARKAINCMALSKGEERLAIAYDSIVLVLDISPGDPCPVIDGPTYTFYTQLPEAISSVAVLADYRVIYGMTNGDLFLYECANSKVFPLEAHGSQVTCMQVSHKEQLAVSGSEEALLCLWDLQACKWRFQMSYTNSYCRGVQCVCFSKDDKYVYAGLKDRSIIVWSVLDGTLLAVQFVHAVVNRIIPTSNGFIAPTRHGYLIRERFQCPSSRVSQQDPLKNFKKAVWKVKSRQREELAAAAGALQDSRSASAQGNERKSNKRSQVCLLV

Target ORF information:

RefSeq Version	XM_022406920.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris NACHT and WD repeat domain containing 1 (NWD1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022406920.1

ORF Insert Sequence:

ATGGTGGACC GGCTCGCAGA TGGCTGCCTG GACACAGATG CCCAGAACCT TCTCAGTGGC 
CTCAAGGGAC GCATTGCCGA CACACAACCT GGAGTCCTCA AGGCCCACCA CCTGCCATGG 
AGCCGAGACC TGGTGAACCC CAAAAACAAG GCTCATGCTC GGTACCTGAA AGAGCTGGGG 
GAGCAGTTTG TGGCCAGGGC TAACCACCAG GTCCTTGAGC ACCTCCGGGA GCAGGAGGCA 
GGCAGGCAGG AGTTGGCGTG GCTCTACCAG GAGATCCGCC ACCACCTGGG GCTGAGCACC 
GAGGCCACCT GGACCTTTTG TGGCCGCCAG GAGCTCCTGG CCCAGCTGGG GCAGCGGCTC 
CGGCAGAGCG ATGGCCATCC GCACTCACCC CTGGTGCTTT TTGGACCTCC AGGCATCGGA 
AAGACAGCGC TGATGTGCAA GTTGGCAGAA CAAATGCCGG GACTGCTTGG CCGCAAGACA 
GTGACCGTCC TGCGGCTTCT GGGGATGTCA CAGATGAGCT CCGACGCCCG AGGCCTGCTC 
CAGAGCATCT GCTTCCAGGT GTGCCTGGCC TACGGGCTGC CCCTGCCCCC TGCCCAGGTC 
CTGGAGGCCC ATGCCAGGGT GGTCCAGTTT TTCCACACCC TCCTCCACAC TGTCTCCTGC 
CGAAACTTCG AGTCACTTGT GATCCTGTTG GACTCCATGG ATGATGTGGA TTCCATCTGC 
CGCGCCCGGA GGGTCCCCTG GCTGCCTCCC CGGTGCCCCC CGAGGATCCA CCTTATCCTC 
TCAGCCTGCT CGGGGAAGCC GGGGATTCTA GACATCATGA GACAGACGCT GACGGACCCA 
GAGGCCTACT GGGAGGTGAA ACCCCTCTCC GGAAACCAAG GGCAGGAGAT GATCCAGCTC 
CTGCTGGCCG CCTCGCGGAG GACGCTGAGC CCAGGGCAGA GGGAGCTGCT CTGGGCCAGC 
CTCCCAGAGT GTGGGCACCC AGGACGGCTG AGGCTGGCCT TTGAGGAGGC CCGGAAGTGG 
GCCTCCTTCA CCATACCTGC CCCTCTGGCC TCCACTGCTA AGGAAGCGAT CCATCAGCTG 
TGTGCCCGCC TGGAGCAGAC ACACGGGCAG CTCCTGGTGG CCCACGTGCT GAGCTACATC 
GTGTCCTCCC GGCATGGGCT CTCAGAGGCA GAGCTGAAGG ACGTCCTGTC CTTGGACGAC 
GAGGTCCTGG AGGCGGTGTA CCAGGACTGG ACCCCGCCCA GCAAGGAGCT GTTGCGCTTC 
CCCCCGTTGC TGTGGGTGAG GCTCCGTCGG GATCTCGGCC ACTGCTTGGC CCGGCGGCCG 
GTGGACGGCT TCACGCTCCT GGCCATCGCC CACAGGCAGC TGACTGAGGT GATCCAAGAG 
CGCTACCTGT CTGGGCCCGA GAGAGCCAAG AGGCACGGAG TCTTGGCTGA CTTCTTCTCA 
GGGGCCTGGA GCCAGGGCAC AAAGAAACTC ATCACCCTGC CACTCGTGGG GAAGCCCCTG 
AACCTGGACC GCAAGGTGGC GCCTCAGCCC CTGTGGTTCT CAGACACAGT TGCAAATCTG 
CGGAAGCTGA AGGAGTTGCC CCATCACCTG GTCCACTCAG GCCGCTTCGA AGAGCTGAAG 
CAGGAGGTTC TGGGCAGCAT GAACTGGATT TCCTGCCGGG GCGTCTCTGG GGGCATCGAG 
GGGCTGTTGG AGGACTTTGA CCTGTATGGC CCTCACCTGG ATTGTCCCGA GGTCGGCCTG 
GTCCGGGACG CCCTCCAGCT GTGCCGCCCG GCCCTGGAGC TCCGAGGCAT GGAGAGGAGC 
ATCCTCTACA CTGAACTCCT GGCCAGACTC CATTTCTTCG CCACCTCACA TCCAGCGCTG 
GTGGGACAGC TGTGCCAACA GGCCCAGAGC TGGTTCCGGC TGTGCCCCCA CCCTGTGCTG 
GTACCTCTCG GGGGGTTCCT CCAGCCCCCC GGGGGACCCC TCCGGGCAAC CTTCACTGGC 
TGTCACAAGG GCATCACTGC CATGGGCTGG AGCCTGGAAG AGAAGCTGCT GGTAGTTGGC 
ACCCAGGATG GCCTCATGGC TGTGTGGGAC ATGGAAGAGC AGCAGGTGAT CCACATCCTA 
ACTGGACACA CAGGAGAGGT GAGGTCTGTG AAAGTGTTTG GCAAGGGGAC ACTCGTCCTC 
TCTGCCTCAA AGGACCACAC ACTGCGTTTG TGGAACTTAC TCTCTGGCCA GCAGAAATTC 
ACCATTTGGG ATGGAGGCTC AAAAGATCCC ACTGACCCTG AGATCTGGAG TCTTCATGTG 
GATGAAGCAA AGAAGGTTGT GTATTTGGCA TCTGGCTCAA AGATCAGTGC TTGGAATCTG 
GAAACTGCAG AGCTGGTTTT CCATATCCTG GGAGATGCCT CCGACTGCTG GATGTGCACA 
GCGGTGCTGG CTTCCCGGGC CACGCTGCTG ACCGTGTCTG AGGGCGGTGT GGTCAGTCTG 
TGGAGCTCAG GCACAGGGAA ACTGCAGGGG AGGCACCATT TATCCAGCAT CAAAGAAGAA 
ACACCTACCT GTGGGGTCTC GGTCCAGAAG CAAGGAAAGA TGGTGACTGG GTTCAGCAAA 
GGCTCCATCT CTTTGGTTTC CCCCGGAGGA GATAGCCTGC TGGAGAAGCT TCCAGAAGCC 
GTGAGGTTCC TGGTAGTCTC TGAAGATGAG TCCCTTCTCG CTGCAGGGTT TGGCAGATCC 
GTGCGGATAT TCTTGGCAGA CTCGCAGGGG TTTCATCGAT TTATGGCCAC CGACCTTGAA 
CATGAGGACA CCGTGGAGAT AGCTGGTTTT GGTCCTGAGA ACAATCTGAT TGTCACTGCG 
TCCCGCGATG CACTCATCCA GGTGTGGAGC CTGTCGGAGC AGGGGACCCT GCTGGACATC 
CTGGAAGGTG TCGGAGCCCC CGTGAGCCTG CTGGCCTGGG CTGGGGCCCT GGTGGCATCT 
GCTTCTCAGC AGTCCTCATC TTTGAAAGTC TGGGATCTCA CTTACATTCA GAAGCCGAGG 
GCCTGCACCC CATTTCTGGA TCGTACTGGC CTCACTGCTG TGTCACACAA TGGAAGCTAC 
GTTTACTTCC CCAAAATCGG AGACAAGAAC AAAGTTACCA TTTGGGACTC TGCAGAAGGC 
GAGGAACAAG ATGCCCTGGA CACCTCCAAT GAGGTCAGGT GTCTGGAGGT TGCCCAGCAG 
GCCAATCTCC TTTTCACCGG CCTCGTTTCA GGGGTTGTCC TGGTGTTTCC CCTGAATTCC 
AGACAGGATG TGATGTGCAT CCCCCCTCCC GAGGCCCGGA AAGCCATCAA CTGCATGGCC 
CTCAGTAAAG GCGAGGAGCG CCTGGCCATC GCCTATGACA GCATTGTCCT TGTGCTGGAC 
ATCAGTCCCG GGGATCCCTG TCCGGTTATT GATGGGCCAA CATACACCTT CTATACCCAG 
CTGCCTGAGG CCATCTCCAG TGTGGCCGTT CTGGCTGACT ACCGCGTGAT CTACGGCATG 
ACCAACGGTG ACCTCTTTCT TTACGAGTGT GCGAATTCCA AAGTGTTCCC TCTGGAGGCC 
CACGGGAGCC AGGTCACCTG CATGCAGGTC AGCCACAAGG AGCAGCTGGC TGTCAGCGGG 
TCCGAGGAAG CCCTGCTGTG TCTCTGGGAC CTGCAGGCCT GCAAGTGGAG GTTCCAGATG 
AGCTACACGA ATTCTTACTG TAGAGGAGTC CAGTGTGTCT GCTTCTCCAA GGATGACAAG 
TATGTGTATG CGGGCTTGAA GGATCGCTCC ATCATTGTCT GGAGTGTGCT GGATGGCACC 
CTGCTGGCTG TCCAGTTTGT CCATGCTGTG GTGAACAGGA TCATCCCAAC TTCGAATGGC 
TTCATTGCCC CCACTAGACA TGGCTACCTG ATCCGCGAGC GTTTCCAGTG CCCTTCATCG 
AGAGTCTCAC AGCAAGATCC TCTCAAGAAC TTCAAGAAGG CAGTGTGGAA GGTCAAATCC 
AGGCAGAGAG AAGAGCTGGC AGCAGCTGCA GGAGCACTCC AGGACTCGAG ATCGGCAAGT 
GCCCAGGGAA ATGAACGTAA ATCGAACAAA CGCTCACAAG TCTGCCTGCT AGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NWD1 ( XM_022406916.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022262624.1 Canis lupus familiaris NACHT and WD repeat domain containing 1 (NWD1), transcript variant X1, mRNA.

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CloneID	OCe295548
Clone ID Related Accession (Same CDS sequence)	XM_022406916.1
Accession Version	XM_022406916.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	NACHT domain- and WD repeat-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGG GCCCCCTCAG CCCGGGCACC CGCCAGCGAC TTCTCCAGGG TCAGGCAGAC 
CCCCTCCCAG CGCCTCTGTC CAGAAGTGTG GCCATCTTCA TCAGCTCCAC GGTCTCGGAT 
ATGGATGCAG AGAGAGAAGC TCTGCAGAGC ACTGCCTTCC CCGAAGTGCA GACCTTCTGC 
CAAAAGAATG GCCTGATGTT CGAGGTTGTT GACCTGAGGT GGGGTATTCG GAACACTGAG 
GCCACTGACC ACATGACCAC AGAACTCTGC TTGGAGGAGC TCGACCGGTG TCGGAAAACA 
TCCATAGGGC CGGCTTTTGT TGCCCTCTTA GGTGACCAGT ACGGCCCCTG TCCAGTCCCT 
ACACTGATCG AGGAGAAGGA GTGGGAGGCC CTGAGATCCC AGCTGGCCTC CAAGCCACGT 
GACCTGGAGC TGGTGGCTCG ACGCTTCCGG AGGGATGAGA ACGCCGTGCC CCCCACCTAC 
CTGCTTCAGG CCCTAGGCAC TGCGGAGACC CAGGGGCCCG AAGAGGCCAC CCTGACCTCT 
GTGCTGCGCT CCGGAGCCCA GGAGGCCCGG AGGCTGGGCC TCATCACCCA GGAGCAGTGG 
CACCGCTACC ACTGGTCAGT CATTGAGTGG GAGATAGACC GGGGCCTGCT GAGCTTGACA 
GACGGAGACC AGGGGGCGAC TGTCTTCTTG AGAGAGATCC AAGACCTCAA CAAACACATC 
CTGGAAGACT GCGCCCTCAA GATGGTGGAC CGGCTCGCAG ATGGCTGCCT GGACACAGAT 
GCCCAGAACC TTCTCAGTGG CCTCAAGGGA CGCATTGCCG ACACACAACC TGGAGTCCTC 
AAGGCCCACC ACCTGCCATG GAGCCGAGAC CTGGTGAACC CCAAAAACAA GGCTCATGCT 
CGGTACCTGA AAGAGCTGGG GGAGCAGTTT GTGGCCAGGG CTAACCACCA GGTCCTTGAG 
CACCTCCGGG AGCAGGAGGC AGGCAGGCAG GAGTTGGCGT GGCTCTACCA GGAGATCCGC 
CACCACCTGG GGCTGAGCAC CGAGGCCACC TGGACCTTTT GTGGCCGCCA GGAGCTCCTG 
GCCCAGCTGG GGCAGCGGCT CCGGCAGAGC GATGGCCATC CGCACTCACC CCTGGTGCTT 
TTTGGACCTC CAGGCATCGG AAAGACAGCG CTGATGTGCA AGTTGGCAGA ACAAATGCCG 
GGACTGCTTG GCCGCAAGAC AGTGACCGTC CTGCGGCTTC TGGGGATGTC ACAGATGAGC 
TCCGACGCCC GAGGCCTGCT CCAGAGCATC TGCTTCCAGG TGTGCCTGGC CTACGGGCTG 
CCCCTGCCCC CTGCCCAGGT CCTGGAGGCC CATGCCAGGG TGGTCCAGTT TTTCCACACC 
CTCCTCCACA CTGTCTCCTG CCGAAACTTC GAGTCACTTG TGATCCTGTT GGACTCCATG 
GATGATGTGG ATTCCATCTG CCGCGCCCGG AGGGTCCCCT GGCTGCCTCC CCGGTGCCCC 
CCGAGGATCC ACCTTATCCT CTCAGCCTGC TCGGGGAAGC CGGGGATTCT AGACATCATG 
AGACAGACGC TGACGGACCC AGAGGCCTAC TGGGAGGTGA AACCCCTCTC CGGAAACCAA 
GGGCAGGAGA TGATCCAGCT CCTGCTGGCC GCCTCGCGGA GGACGCTGAG CCCAGGGCAG 
AGGGAGCTGC TCTGGGCCAG CCTCCCAGAG TGTGGGCACC CAGGACGGCT GAGGCTGGCC 
TTTGAGGAGG CCCGGAAGTG GGCCTCCTTC ACCATACCTG CCCCTCTGGC CTCCACTGCT 
AAGGAAGCGA TCCATCAGCT GTGTGCCCGC CTGGAGCAGA CACACGGGCA GCTCCTGGTG 
GCCCACGTGC TGAGCTACAT CGTGTCCTCC CGGCATGGGC TCTCAGAGGC AGAGCTGAAG 
GACGTCCTGT CCTTGGACGA CGAGGTCCTG GAGGCGGTGT ACCAGGACTG GACCCCGCCC 
AGCAAGGAGC TGTTGCGCTT CCCCCCGTTG CTGTGGGTGA GGCTCCGTCG GGATCTCGGC 
CACTGCTTGG CCCGGCGGCC GGTGGACGGC TTCACGCTCC TGGCCATCGC CCACAGGCAG 
CTGACTGAGG TGATCCAAGA GCGCTACCTG TCTGGGCCCG AGAGAGCCAA GAGGCACGGA 
GTCTTGGCTG ACTTCTTCTC AGGGGCCTGG AGCCAGGGCA CAAAGAAACT CATCACCCTG 
CCACTCGTGG GGAAGCCCCT GAACCTGGAC CGCAAGGTGG CGCCTCAGCC CCTGTGGTTC 
TCAGACACAG TTGCAAATCT GCGGAAGCTG AAGGAGTTGC CCCATCACCT GGTCCACTCA 
GGCCGCTTCG AAGAGCTGAA GCAGGAGGTT CTGGGCAGCA TGAACTGGAT TTCCTGCCGG 
GGCGTCTCTG GGGGCATCGA GGGGCTGTTG GAGGACTTTG ACCTGTATGG CCCTCACCTG 
GATTGTCCCG AGGTCGGCCT GGTCCGGGAC GCCCTCCAGC TGTGCCGCCC GGCCCTGGAG 
CTCCGAGGCA TGGAGAGGAG CATCCTCTAC ACTGAACTCC TGGCCAGACT CCATTTCTTC 
GCCACCTCAC ATCCAGCGCT GGTGGGACAG CTGTGCCAAC AGGCCCAGAG CTGGTTCCGG 
CTGTGCCCCC ACCCTGTGCT GGTACCTCTC GGGGGGTTCC TCCAGCCCCC CGGGGGACCC 
CTCCGGGCAA CCTTCACTGG CTGTCACAAG GGCATCACTG CCATGGGCTG GAGCCTGGAA 
GAGAAGCTGC TGGTAGTTGG CACCCAGGAT GGCCTCATGG CTGTGTGGGA CATGGAAGAG 
CAGCAGGTGA TCCACATCCT AACTGGACAC ACAGGAGAGG TGAGGTCTGT GAAAGTGTTT 
GGCAAGGGGA CACTCGTCCT CTCTGCCTCA AAGGACCACA CACTGCGTTT GTGGAACTTA 
CTCTCTGGCC AGCAGAAATT CACCATTTGG GATGGAGGCT CAAAAGATCC CACTGACCCT 
GAGATCTGGA GTCTTCATGT GGATGAAGCA AAGAAGGTTG TGTATTTGGC ATCTGGCTCA 
AAGATCAGTG CTTGGAATCT GGAAACTGCA GAGCTGGTTT TCCATATCCT GGGAGATGCC 
TCCGACTGCT GGATGTGCAC AGCGGTGCTG GCTTCCCGGG CCACGCTGCT GACCGTGTCT 
GAGGGCGGTG TGGTCAGTCT GTGGAGCTCA GGCACAGGGA AACTGCAGGG GAGGCACCAT 
TTATCCAGCA TCAAAGAAGA AACACCTACC TGTGGGGTCT CGGTCCAGAA GCAAGGAAAG 
ATGGTGACTG GGTTCAGCAA AGGCTCCATC TCTTTGGTTT CCCCCGGAGG AGATAGCCTG 
CTGGAGAAGC TTCCAGAAGC CGTGAGGTTC CTGGTAGTCT CTGAAGATGA GTCCCTTCTC 
GCTGCAGGGT TTGGCAGATC CGTGCGGATA TTCTTGGCAG ACTCGCAGGG GTTTCATCGA 
TTTATGGCCA CCGACCTTGA ACATGAGGAC ACCGTGGAGA TAGCTGGTTT TGGTCCTGAG 
AACAATCTGA TTGTCACTGC GTCCCGCGAT GCACTCATCC AGGTGTGGAG CCTGTCGGAG 
CAGGGGACCC TGCTGGACAT CCTGGAAGGT GTCGGAGCCC CCGTGAGCCT GCTGGCCTGG 
GCTGGGGCCC TGGTGGCATC TGCTTCTCAG CAGTCCTCAT CTTTGAAAGT CTGGGATCTC 
ACTTACATTC AGAAGCCGAG GGCCTGCACC CCATTTCTGG ATCGTACTGG CCTCACTGCT 
GTGTCACACA ATGGAAGCTA CGTTTACTTC CCCAAAATCG GAGACAAGAA CAAAGTTACC 
ATTTGGGACT CTGCAGAAGG CGAGGAACAA GATGCCCTGG ACACCTCCAA TGAGGTCAGG 
TGTCTGGAGG TTGCCCAGCA GGCCAATCTC CTTTTCACCG GCCTCGTTTC AGGGGTTGTC 
CTGGTGTTTC CCCTGAATTC CAGACAGGAT GTGATGTGCA TCCCCCCTCC CGAGGCCCGG 
AAAGCCATCA ACTGCATGGC CCTCAGTAAA GGCGAGGAGC GCCTGGCCAT CGCCTATGAC 
AGCATTGTCC TTGTGCTGGA CATCAGTCCC GGGGATCCCT GTCCGGTTAT TGATGGGCCA 
ACATACACCT TCTATACCCA GCTGCCTGAG GCCATCTCCA GTGTGGCCGT TCTGGCTGAC 
TACCGCGTGA TCTACGGCAT GACCAACGGT GACCTCTTTC TTTACGAGTG TGCGAATTCC 
AAAGTGTTCC CTCTGGAGGC CCACGGGAGC CAGGTCACCT GCATGCAGGT CAGCCACAAG 
GAGCAGCTGG CTGTCAGCGG GTCCGAGGAA GCCCTGCTGT GTCTCTGGGA CCTGCAGGCC 
TGCAAGTGGA GGTTCCAGAT GAGCTACACG AATTCTTACT GTAGAGGAGT CCAGTGTGTC 
TGCTTCTCCA AGGATGACAA GTATGTGTAT GCGGGCTTGA AGGATCGCTC CATCATTGTC 
TGGAGTGTGC TGGATGGCAC CCTGCTGGCT GTCCAGTTTG TCCATGCTGT GGTGAACAGG 
ATCATCCCAA CTTCGAATGG CTTCATTGCC CCCACTAGAC ATGGCTACCT GATCCGCGAG 
CGTTTCCAGT GCCCTTCATC GAGAGTCTCA CAGCAAGATC CTCTCAAGAA CTTCAAGAAG 
GCAGTGTGGA AGGTCAAATC CAGGCAGAGA GAAGAGCTGG CAGCAGCTGC AGGAGCACTC 
CAGGACTCGA GATCGGCAAG TGCCCAGGGA AATGAACGTA AATCGAACAA ACGCTCACAA 
GTCTGCCTGC TAGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022262624.1
CDS	249..5066
Translation	MSVGPLSPGTRQRLLQGQADPLPAPLSRSVAIFISSTVSDMDAEREALQSTAFPEVQTFCQKNGLMFEVVDLRWGIRNTEATDHMTTELCLEELDRCRKTSIGPAFVALLGDQYGPCPVPTLIEEKEWEALRSQLASKPRDLELVARRFRRDENAVPPTYLLQALGTAETQGPEEATLTSVLRSGAQEARRLGLITQEQWHRYHWSVIEWEIDRGLLSLTDGDQGATVFLREIQDLNKHILEDCALKMVDRLADGCLDTDAQNLLSGLKGRIADTQPGVLKAHHLPWSRDLVNPKNKAHARYLKELGEQFVARANHQVLEHLREQEAGRQELAWLYQEIRHHLGLSTEATWTFCGRQELLAQLGQRLRQSDGHPHSPLVLFGPPGIGKTALMCKLAEQMPGLLGRKTVTVLRLLGMSQMSSDARGLLQSICFQVCLAYGLPLPPAQVLEAHARVVQFFHTLLHTVSCRNFESLVILLDSMDDVDSICRARRVPWLPPRCPPRIHLILSACSGKPGILDIMRQTLTDPEAYWEVKPLSGNQGQEMIQLLLAASRRTLSPGQRELLWASLPECGHPGRLRLAFEEARKWASFTIPAPLASTAKEAIHQLCARLEQTHGQLLVAHVLSYIVSSRHGLSEAELKDVLSLDDEVLEAVYQDWTPPSKELLRFPPLLWVRLRRDLGHCLARRPVDGFTLLAIAHRQLTEVIQERYLSGPERAKRHGVLADFFSGAWSQGTKKLITLPLVGKPLNLDRKVAPQPLWFSDTVANLRKLKELPHHLVHSGRFEELKQEVLGSMNWISCRGVSGGIEGLLEDFDLYGPHLDCPEVGLVRDALQLCRPALELRGMERSILYTELLARLHFFATSHPALVGQLCQQAQSWFRLCPHPVLVPLGGFLQPPGGPLRATFTGCHKGITAMGWSLEEKLLVVGTQDGLMAVWDMEEQQVIHILTGHTGEVRSVKVFGKGTLVLSASKDHTLRLWNLLSGQQKFTIWDGGSKDPTDPEIWSLHVDEAKKVVYLASGSKISAWNLETAELVFHILGDASDCWMCTAVLASRATLLTVSEGGVVSLWSSGTGKLQGRHHLSSIKEETPTCGVSVQKQGKMVTGFSKGSISLVSPGGDSLLEKLPEAVRFLVVSEDESLLAAGFGRSVRIFLADSQGFHRFMATDLEHEDTVEIAGFGPENNLIVTASRDALIQVWSLSEQGTLLDILEGVGAPVSLLAWAGALVASASQQSSSLKVWDLTYIQKPRACTPFLDRTGLTAVSHNGSYVYFPKIGDKNKVTIWDSAEGEEQDALDTSNEVRCLEVAQQANLLFTGLVSGVVLVFPLNSRQDVMCIPPPEARKAINCMALSKGEERLAIAYDSIVLVLDISPGDPCPVIDGPTYTFYTQLPEAISSVAVLADYRVIYGMTNGDLFLYECANSKVFPLEAHGSQVTCMQVSHKEQLAVSGSEEALLCLWDLQACKWRFQMSYTNSYCRGVQCVCFSKDDKYVYAGLKDRSIIVWSVLDGTLLAVQFVHAVVNRIIPTSNGFIAPTRHGYLIRERFQCPSSRVSQQDPLKNFKKAVWKVKSRQREELAAAAGALQDSRSASAQGNERKSNKRSQVCLLV

Target ORF information:

RefSeq Version	XM_022406916.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris NACHT and WD repeat domain containing 1 (NWD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022406916.1

ORF Insert Sequence:

ATGAGTGTGG GCCCCCTCAG CCCGGGCACC CGCCAGCGAC TTCTCCAGGG TCAGGCAGAC 
CCCCTCCCAG CGCCTCTGTC CAGAAGTGTG GCCATCTTCA TCAGCTCCAC GGTCTCGGAT 
ATGGATGCAG AGAGAGAAGC TCTGCAGAGC ACTGCCTTCC CCGAAGTGCA GACCTTCTGC 
CAAAAGAATG GCCTGATGTT CGAGGTTGTT GACCTGAGGT GGGGTATTCG GAACACTGAG 
GCCACTGACC ACATGACCAC AGAACTCTGC TTGGAGGAGC TCGACCGGTG TCGGAAAACA 
TCCATAGGGC CGGCTTTTGT TGCCCTCTTA GGTGACCAGT ACGGCCCCTG TCCAGTCCCT 
ACACTGATCG AGGAGAAGGA GTGGGAGGCC CTGAGATCCC AGCTGGCCTC CAAGCCACGT 
GACCTGGAGC TGGTGGCTCG ACGCTTCCGG AGGGATGAGA ACGCCGTGCC CCCCACCTAC 
CTGCTTCAGG CCCTAGGCAC TGCGGAGACC CAGGGGCCCG AAGAGGCCAC CCTGACCTCT 
GTGCTGCGCT CCGGAGCCCA GGAGGCCCGG AGGCTGGGCC TCATCACCCA GGAGCAGTGG 
CACCGCTACC ACTGGTCAGT CATTGAGTGG GAGATAGACC GGGGCCTGCT GAGCTTGACA 
GACGGAGACC AGGGGGCGAC TGTCTTCTTG AGAGAGATCC AAGACCTCAA CAAACACATC 
CTGGAAGACT GCGCCCTCAA GATGGTGGAC CGGCTCGCAG ATGGCTGCCT GGACACAGAT 
GCCCAGAACC TTCTCAGTGG CCTCAAGGGA CGCATTGCCG ACACACAACC TGGAGTCCTC 
AAGGCCCACC ACCTGCCATG GAGCCGAGAC CTGGTGAACC CCAAAAACAA GGCTCATGCT 
CGGTACCTGA AAGAGCTGGG GGAGCAGTTT GTGGCCAGGG CTAACCACCA GGTCCTTGAG 
CACCTCCGGG AGCAGGAGGC AGGCAGGCAG GAGTTGGCGT GGCTCTACCA GGAGATCCGC 
CACCACCTGG GGCTGAGCAC CGAGGCCACC TGGACCTTTT GTGGCCGCCA GGAGCTCCTG 
GCCCAGCTGG GGCAGCGGCT CCGGCAGAGC GATGGCCATC CGCACTCACC CCTGGTGCTT 
TTTGGACCTC CAGGCATCGG AAAGACAGCG CTGATGTGCA AGTTGGCAGA ACAAATGCCG 
GGACTGCTTG GCCGCAAGAC AGTGACCGTC CTGCGGCTTC TGGGGATGTC ACAGATGAGC 
TCCGACGCCC GAGGCCTGCT CCAGAGCATC TGCTTCCAGG TGTGCCTGGC CTACGGGCTG 
CCCCTGCCCC CTGCCCAGGT CCTGGAGGCC CATGCCAGGG TGGTCCAGTT TTTCCACACC 
CTCCTCCACA CTGTCTCCTG CCGAAACTTC GAGTCACTTG TGATCCTGTT GGACTCCATG 
GATGATGTGG ATTCCATCTG CCGCGCCCGG AGGGTCCCCT GGCTGCCTCC CCGGTGCCCC 
CCGAGGATCC ACCTTATCCT CTCAGCCTGC TCGGGGAAGC CGGGGATTCT AGACATCATG 
AGACAGACGC TGACGGACCC AGAGGCCTAC TGGGAGGTGA AACCCCTCTC CGGAAACCAA 
GGGCAGGAGA TGATCCAGCT CCTGCTGGCC GCCTCGCGGA GGACGCTGAG CCCAGGGCAG 
AGGGAGCTGC TCTGGGCCAG CCTCCCAGAG TGTGGGCACC CAGGACGGCT GAGGCTGGCC 
TTTGAGGAGG CCCGGAAGTG GGCCTCCTTC ACCATACCTG CCCCTCTGGC CTCCACTGCT 
AAGGAAGCGA TCCATCAGCT GTGTGCCCGC CTGGAGCAGA CACACGGGCA GCTCCTGGTG 
GCCCACGTGC TGAGCTACAT CGTGTCCTCC CGGCATGGGC TCTCAGAGGC AGAGCTGAAG 
GACGTCCTGT CCTTGGACGA CGAGGTCCTG GAGGCGGTGT ACCAGGACTG GACCCCGCCC 
AGCAAGGAGC TGTTGCGCTT CCCCCCGTTG CTGTGGGTGA GGCTCCGTCG GGATCTCGGC 
CACTGCTTGG CCCGGCGGCC GGTGGACGGC TTCACGCTCC TGGCCATCGC CCACAGGCAG 
CTGACTGAGG TGATCCAAGA GCGCTACCTG TCTGGGCCCG AGAGAGCCAA GAGGCACGGA 
GTCTTGGCTG ACTTCTTCTC AGGGGCCTGG AGCCAGGGCA CAAAGAAACT CATCACCCTG 
CCACTCGTGG GGAAGCCCCT GAACCTGGAC CGCAAGGTGG CGCCTCAGCC CCTGTGGTTC 
TCAGACACAG TTGCAAATCT GCGGAAGCTG AAGGAGTTGC CCCATCACCT GGTCCACTCA 
GGCCGCTTCG AAGAGCTGAA GCAGGAGGTT CTGGGCAGCA TGAACTGGAT TTCCTGCCGG 
GGCGTCTCTG GGGGCATCGA GGGGCTGTTG GAGGACTTTG ACCTGTATGG CCCTCACCTG 
GATTGTCCCG AGGTCGGCCT GGTCCGGGAC GCCCTCCAGC TGTGCCGCCC GGCCCTGGAG 
CTCCGAGGCA TGGAGAGGAG CATCCTCTAC ACTGAACTCC TGGCCAGACT CCATTTCTTC 
GCCACCTCAC ATCCAGCGCT GGTGGGACAG CTGTGCCAAC AGGCCCAGAG CTGGTTCCGG 
CTGTGCCCCC ACCCTGTGCT GGTACCTCTC GGGGGGTTCC TCCAGCCCCC CGGGGGACCC 
CTCCGGGCAA CCTTCACTGG CTGTCACAAG GGCATCACTG CCATGGGCTG GAGCCTGGAA 
GAGAAGCTGC TGGTAGTTGG CACCCAGGAT GGCCTCATGG CTGTGTGGGA CATGGAAGAG 
CAGCAGGTGA TCCACATCCT AACTGGACAC ACAGGAGAGG TGAGGTCTGT GAAAGTGTTT 
GGCAAGGGGA CACTCGTCCT CTCTGCCTCA AAGGACCACA CACTGCGTTT GTGGAACTTA 
CTCTCTGGCC AGCAGAAATT CACCATTTGG GATGGAGGCT CAAAAGATCC CACTGACCCT 
GAGATCTGGA GTCTTCATGT GGATGAAGCA AAGAAGGTTG TGTATTTGGC ATCTGGCTCA 
AAGATCAGTG CTTGGAATCT GGAAACTGCA GAGCTGGTTT TCCATATCCT GGGAGATGCC 
TCCGACTGCT GGATGTGCAC AGCGGTGCTG GCTTCCCGGG CCACGCTGCT GACCGTGTCT 
GAGGGCGGTG TGGTCAGTCT GTGGAGCTCA GGCACAGGGA AACTGCAGGG GAGGCACCAT 
TTATCCAGCA TCAAAGAAGA AACACCTACC TGTGGGGTCT CGGTCCAGAA GCAAGGAAAG 
ATGGTGACTG GGTTCAGCAA AGGCTCCATC TCTTTGGTTT CCCCCGGAGG AGATAGCCTG 
CTGGAGAAGC TTCCAGAAGC CGTGAGGTTC CTGGTAGTCT CTGAAGATGA GTCCCTTCTC 
GCTGCAGGGT TTGGCAGATC CGTGCGGATA TTCTTGGCAG ACTCGCAGGG GTTTCATCGA 
TTTATGGCCA CCGACCTTGA ACATGAGGAC ACCGTGGAGA TAGCTGGTTT TGGTCCTGAG 
AACAATCTGA TTGTCACTGC GTCCCGCGAT GCACTCATCC AGGTGTGGAG CCTGTCGGAG 
CAGGGGACCC TGCTGGACAT CCTGGAAGGT GTCGGAGCCC CCGTGAGCCT GCTGGCCTGG 
GCTGGGGCCC TGGTGGCATC TGCTTCTCAG CAGTCCTCAT CTTTGAAAGT CTGGGATCTC 
ACTTACATTC AGAAGCCGAG GGCCTGCACC CCATTTCTGG ATCGTACTGG CCTCACTGCT 
GTGTCACACA ATGGAAGCTA CGTTTACTTC CCCAAAATCG GAGACAAGAA CAAAGTTACC 
ATTTGGGACT CTGCAGAAGG CGAGGAACAA GATGCCCTGG ACACCTCCAA TGAGGTCAGG 
TGTCTGGAGG TTGCCCAGCA GGCCAATCTC CTTTTCACCG GCCTCGTTTC AGGGGTTGTC 
CTGGTGTTTC CCCTGAATTC CAGACAGGAT GTGATGTGCA TCCCCCCTCC CGAGGCCCGG 
AAAGCCATCA ACTGCATGGC CCTCAGTAAA GGCGAGGAGC GCCTGGCCAT CGCCTATGAC 
AGCATTGTCC TTGTGCTGGA CATCAGTCCC GGGGATCCCT GTCCGGTTAT TGATGGGCCA 
ACATACACCT TCTATACCCA GCTGCCTGAG GCCATCTCCA GTGTGGCCGT TCTGGCTGAC 
TACCGCGTGA TCTACGGCAT GACCAACGGT GACCTCTTTC TTTACGAGTG TGCGAATTCC 
AAAGTGTTCC CTCTGGAGGC CCACGGGAGC CAGGTCACCT GCATGCAGGT CAGCCACAAG 
GAGCAGCTGG CTGTCAGCGG GTCCGAGGAA GCCCTGCTGT GTCTCTGGGA CCTGCAGGCC 
TGCAAGTGGA GGTTCCAGAT GAGCTACACG AATTCTTACT GTAGAGGAGT CCAGTGTGTC 
TGCTTCTCCA AGGATGACAA GTATGTGTAT GCGGGCTTGA AGGATCGCTC CATCATTGTC 
TGGAGTGTGC TGGATGGCAC CCTGCTGGCT GTCCAGTTTG TCCATGCTGT GGTGAACAGG 
ATCATCCCAA CTTCGAATGG CTTCATTGCC CCCACTAGAC ATGGCTACCT GATCCGCGAG 
CGTTTCCAGT GCCCTTCATC GAGAGTCTCA CAGCAAGATC CTCTCAAGAA CTTCAAGAAG 
GCAGTGTGGA AGGTCAAATC CAGGCAGAGA GAAGAGCTGG CAGCAGCTGC AGGAGCACTC 
CAGGACTCGA GATCGGCAAG TGCCCAGGGA AATGAACGTA AATCGAACAA ACGCTCACAA 
GTCTGCCTGC TAGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NWD1 ( XM_022406917.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022262625.1 Canis lupus familiaris NACHT and WD repeat domain containing 1 (NWD1), transcript variant X2, mRNA.

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CloneID	OCe295549
Clone ID Related Accession (Same CDS sequence)	XM_022406917.1
Accession Version	XM_022406917.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	NACHT domain- and WD repeat-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGG GCCCCCTCAG CCCGGGCACC CGCCAGCGAC TTCTCCAGGG TCAGGCAGAC 
CCCCTCCCAG CGCCTCTGTC CAGAAGTGTG GCCATCTTCA TCAGCTCCAC GGTCTCGGAT 
ATGGATGCAG AGAGAGAAGC TCTGCAGAGC ACTGCCTTCC CCGAAGTGCA GACCTTCTGC 
CAAAAGAATG GCCTGATGTT CGAGGTTGTT GACCTGAGGT GGGGTATTCG GAACACTGAG 
GCCACTGACC ACATGACCAC AGAACTCTGC TTGGAGGAGC TCGACCGGTG TCGGAAAACA 
TCCATAGGGC CGGCTTTTGT TGCCCTCTTA GGTGACCAGT ACGGCCCCTG TCCAGTCCCT 
ACACTGATCG AGGAGAAGGA GTGGGAGGCC CTGAGATCCC AGCTGGCCTC CAAGCCACGT 
GACCTGGAGC TGGTGGCTCG ACGCTTCCGG AGGGATGAGA ACGCCGTGCC CCCCACCTAC 
CTGCTTCAGG CCCTAGGCAC TGCGGAGACC CAGGGGCCCG AAGAGGCCAC CCTGACCTCT 
GTGCTGCGCT CCGGAGCCCA GGAGGCCCGG AGGCTGGGCC TCATCACCCA GGAGCAGTGG 
CACCGCTACC ACTGGTCAGT CATTGAGTGG GAGATAGACC GGGGCCTGCT GAGCTTGACA 
GACGGAGACC AGGGGGCGAC TGTCTTCTTG AGAGAGATCC AAGACCTCAA CAAACACATC 
CTGGAAGACT GCGCCCTCAA GATGGTGGAC CGGCTCGCAG ATGGCTGCCT GGACACAGAT 
GCCCAGAACC TTCTCAGTGG CCTCAAGGGA CGCATTGCCG ACACACAACC TGGAGTCCTC 
AAGGCCCACC ACCTGCCATG GAGCCGAGAC CTGGTGAACC CCAAAAACAA GGCTCATGCT 
CGGTACCTGA AAGAGCTGGG GGAGCAGTTT GTGGCCAGGG CTAACCACCA GGTCCTTGAG 
CACCTCCGGG AGCAGGAGGC AGGCAGGCAG GAGTTGGCGT GGCTCTACCA GGAGATCCGC 
CACCACCTGG GGCTGAGCAC CGAGGCCACC TGGACCTTTT GTGGCCGCCA GGAGCTCCTG 
GCCCAGCTGG GGCAGCGGCT CCGGCAGAGC GATGGCCATC CGCACTCACC CCTGGTGCTT 
TTTGGACCTC CAGGCATCGG AAAGACAGCG CTGATGTGCA AGTTGGCAGA ACAAATGCCG 
GGACTGCTTG GCCGCAAGAC AGTGACCGTC CTGCGGCTTC TGGGGATGTC ACAGATGAGC 
TCCGACGCCC GAGGCCTGCT CCAGAGCATC TGCTTCCAGG TGTGCCTGGC CTACGGGCTG 
CCCCTGCCCC CTGCCCAGGT CCTGGAGGCC CATGCCAGGG TGGTCCAGTT TTTCCACACC 
CTCCTCCACA CTGTCTCCTG CCGAAACTTC GAGTCACTTG TGATCCTGTT GGACTCCATG 
GATGATGTGG ATTCCATCTG CCGCGCCCGG AGGGTCCCCT GGCTGCCTCC CCGGTGCCCC 
CCGAGGATCC ACCTTATCCT CTCAGCCTGC TCGGGGAAGC CGGGGATTCT AGACATCATG 
AGACAGACGC TGACGGACCC AGAGGCCTAC TGGGAGGTGA AACCCCTCTC CGGAAACCAA 
GGGCAGGAGA TGATCCAGCT CCTGCTGGCC GCCTCGCGGA GGACGCTGAG CCCAGGGCAG 
AGGGAGCTGC TCTGGGCCAG CCTCCCAGAG TGTGGGCACC CAGGACGGCT GAGGCTGGCC 
TTTGAGGAGG CCCGGAAGTG GGCCTCCTTC ACCATACCTG CCCCTCTGGC CTCCACTGCT 
AAGGAAGCGA TCCATCAGCT GTGTGCCCGC CTGGAGCAGA CACACGGGCA GCTCCTGGTG 
GCCCACGTGC TGAGCTACAT CGTGTCCTCC CGGCATGGGC TCTCAGAGGC AGAGCTGAAG 
GACGTCCTGT CCTTGGACGA CGAGGTCCTG GAGGCGGTGT ACCAGGACTG GACCCCGCCC 
AGCAAGGAGC TGTTGCGCTT CCCCCCGTTG CTGTGGGTGA GGCTCCGTCG GGATCTCGGC 
CACTGCTTGG CCCGGCGGCC GGTGGACGGC TTCACGCTCC TGGCCATCGC CCACAGGCAG 
CTGACTGAGG TGATCCAAGA GCGCTACCTG TCTGGGCCCG AGAGAGCCAA GAGGCACGGA 
GTCTTGGCTG ACTTCTTCTC AGGGGCCTGG AGCCAGGGCA CAAAGAAACT CATCACCCTG 
CCACTCGTGG GGAAGCCCCT GAACCTGGAC CGCAAGGTGG CGCCTCAGCC CCTGTGGTTC 
TCAGACACAG TTGCAAATCT GCGGAAGCTG AAGGAGTTGC CCCATCACCT GGTCCACTCA 
GGCCGCTTCG AAGAGCTGAA GCAGGAGGTT CTGGGCAGCA TGAACTGGAT TTCCTGCCGG 
GGCGTCTCTG GGGGCATCGA GGGGCTGTTG GAGGACTTTG ACCTGTATGG CCCTCACCTG 
GATTGTCCCG AGGTCGGCCT GGTCCGGGAC GCCCTCCAGC TGTGCCGCCC GGCCCTGGAG 
CTCCGAGGCA TGGAGAGGAG CATCCTCTAC ACTGAACTCC TGGCCAGACT CCATTTCTTC 
GCCACCTCAC ATCCAGCGCT GGTGGGACAG CTGTGCCAAC AGGCCCAGAG CTGGTTCCGG 
CTGTGCCCCC ACCCTGTGCT GGTACCTCTC GGGGGGTTCC TCCAGCCCCC CGGGGGACCC 
CTCCGGGCAA CCTTCACTGG CTGTCACAAG GGCATCACTG CCATGGGCTG GAGCCTGGAA 
GAGAAGCTGC TGGTAGTTGG CACCCAGGAT GGCCTCATGG CTGTGTGGGA CATGGAAGAG 
CAGCAGGTGA TCCACATCCT AACTGGACAC ACAGGAGAGG TGAGGTCTGT GAAAGTGTTT 
GGCAAGGGGA CACTCGTCCT CTCTGCCTCA AAGGACCACA CACTGCGTTT GTGGAACTTA 
CTCTCTGGCC AGCAGAAATT CACCATTTGG GATGGAGGCT CAAAAGATCC CACTGACCCT 
GAGATCTGGA GTCTTCATGT GGATGAAGCA AAGAAGGTTG TGTATTTGGC ATCTGGCTCA 
AAGATCAGTG CTTGGAATCT GGAAACTGCA GAGCTGGTTT TCCATATCCT GGGAGATGCC 
TCCGACTGCT GGATGTGCAC AGCGGTGCTG GCTTCCCGGG CCACGCTGCT GACCGTGTCT 
GAGGGCGGTG TGGTCAGTCT GTGGAGCTCA GGCACAGGGA AACTGCAGGG GAGGCACCAT 
TTATCCAGCA TCAAAGAAGA AACACCTACC TGTGGGGTCT CGGTCCAGAA GCAAGGAAAG 
ATGGTGACTG GGTTCAGCAA AGGCTCCATC TCTTTGGTTT CCCCCGGAGG AGATAGCCTG 
CTGGAGAAGC TTCCAGAAGC CGTGAGGTTC CTGGTAGTCT CTGAAGATGA GTCCCTTCTC 
GCTGCAGGGT TTGGCAGATC CGTGCGGATA TTCTTGGCAG ACTCGCAGGG GTTTCATCGA 
TTTATGGCCA CCGACCTTGA ACATGAGGAC ACCGTGGAGA TAGCTGGTTT TGGTCCTGAG 
AACAATCTGA TTGTCACTGC GTCCCGCGAT GCACTCATCC AGGTGTGGAG CCTGTCGGAG 
CAGGGGACCC TGCTGGACAT CCTGGAAGGT GTCGGAGCCC CCGTGAGCCT GCTGGCCTGG 
GCTGGGGCCC TGGTGGCATC TGCTTCTCAG CAGTCCTCAT CTTTGAAAGT CTGGGATCTC 
ACTTACATTC AGAAGCCGAG GGCCTGCACC CCATTTCTGG ATCGTACTGG CCTCACTGCT 
GTGTCACACA ATGGAAGCTA CGTTTACTTC CCCAAAATCG GAGACAAGAA CAAAGTTACC 
ATTTGGGACT CTGCAGAAGG GGTTGTCCTG GTGTTTCCCC TGAATTCCAG ACAGGATGTG 
ATGTGCATCC CCCCTCCCGA GGCCCGGAAA GCCATCAACT GCATGGCCCT CAGTAAAGGC 
GAGGAGCGCC TGGCCATCGC CTATGACAGC ATTGTCCTTG TGCTGGACAT CAGTCCCGGG 
GATCCCTGTC CGGTTATTGA TGGGCCAACA TACACCTTCT ATACCCAGCT GCCTGAGGCC 
ATCTCCAGTG TGGCCGTTCT GGCTGACTAC CGCGTGATCT ACGGCATGAC CAACGGTGAC 
CTCTTTCTTT ACGAGTGTGC GAATTCCAAA GTGTTCCCTC TGGAGGCCCA CGGGAGCCAG 
GTCACCTGCA TGCAGGTCAG CCACAAGGAG CAGCTGGCTG TCAGCGGGTC CGAGGAAGCC 
CTGCTGTGTC TCTGGGACCT GCAGGCCTGC AAGTGGAGGT TCCAGATGAG CTACACGAAT 
TCTTACTGTA GAGGAGTCCA GTGTGTCTGC TTCTCCAAGG ATGACAAGTA TGTGTATGCG 
GGCTTGAAGG ATCGCTCCAT CATTGTCTGG AGTGTGCTGG ATGGCACCCT GCTGGCTGTC 
CAGTTTGTCC ATGCTGTGGT GAACAGGATC ATCCCAACTT CGAATGGCTT CATTGCCCCC 
ACTAGACATG GCTACCTGAT CCGCGAGCGT TTCCAGTGCC CTTCATCGAG AGTCTCACAG 
CAAGATCCTC TCAAGAACTT CAAGAAGGCA GTGTGGAAGG TCAAATCCAG GCAGAGAGAA 
GAGCTGGCAG CAGCTGCAGG AGCACTCCAG GACTCGAGAT CGGCAAGTGC CCAGGGAAAT 
GAACGTAAAT CGAACAAACG CTCACAAGTC TGCCTGCTAG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022262625.1
CDS	252..4976
Translation	MSVGPLSPGTRQRLLQGQADPLPAPLSRSVAIFISSTVSDMDAEREALQSTAFPEVQTFCQKNGLMFEVVDLRWGIRNTEATDHMTTELCLEELDRCRKTSIGPAFVALLGDQYGPCPVPTLIEEKEWEALRSQLASKPRDLELVARRFRRDENAVPPTYLLQALGTAETQGPEEATLTSVLRSGAQEARRLGLITQEQWHRYHWSVIEWEIDRGLLSLTDGDQGATVFLREIQDLNKHILEDCALKMVDRLADGCLDTDAQNLLSGLKGRIADTQPGVLKAHHLPWSRDLVNPKNKAHARYLKELGEQFVARANHQVLEHLREQEAGRQELAWLYQEIRHHLGLSTEATWTFCGRQELLAQLGQRLRQSDGHPHSPLVLFGPPGIGKTALMCKLAEQMPGLLGRKTVTVLRLLGMSQMSSDARGLLQSICFQVCLAYGLPLPPAQVLEAHARVVQFFHTLLHTVSCRNFESLVILLDSMDDVDSICRARRVPWLPPRCPPRIHLILSACSGKPGILDIMRQTLTDPEAYWEVKPLSGNQGQEMIQLLLAASRRTLSPGQRELLWASLPECGHPGRLRLAFEEARKWASFTIPAPLASTAKEAIHQLCARLEQTHGQLLVAHVLSYIVSSRHGLSEAELKDVLSLDDEVLEAVYQDWTPPSKELLRFPPLLWVRLRRDLGHCLARRPVDGFTLLAIAHRQLTEVIQERYLSGPERAKRHGVLADFFSGAWSQGTKKLITLPLVGKPLNLDRKVAPQPLWFSDTVANLRKLKELPHHLVHSGRFEELKQEVLGSMNWISCRGVSGGIEGLLEDFDLYGPHLDCPEVGLVRDALQLCRPALELRGMERSILYTELLARLHFFATSHPALVGQLCQQAQSWFRLCPHPVLVPLGGFLQPPGGPLRATFTGCHKGITAMGWSLEEKLLVVGTQDGLMAVWDMEEQQVIHILTGHTGEVRSVKVFGKGTLVLSASKDHTLRLWNLLSGQQKFTIWDGGSKDPTDPEIWSLHVDEAKKVVYLASGSKISAWNLETAELVFHILGDASDCWMCTAVLASRATLLTVSEGGVVSLWSSGTGKLQGRHHLSSIKEETPTCGVSVQKQGKMVTGFSKGSISLVSPGGDSLLEKLPEAVRFLVVSEDESLLAAGFGRSVRIFLADSQGFHRFMATDLEHEDTVEIAGFGPENNLIVTASRDALIQVWSLSEQGTLLDILEGVGAPVSLLAWAGALVASASQQSSSLKVWDLTYIQKPRACTPFLDRTGLTAVSHNGSYVYFPKIGDKNKVTIWDSAEGVVLVFPLNSRQDVMCIPPPEARKAINCMALSKGEERLAIAYDSIVLVLDISPGDPCPVIDGPTYTFYTQLPEAISSVAVLADYRVIYGMTNGDLFLYECANSKVFPLEAHGSQVTCMQVSHKEQLAVSGSEEALLCLWDLQACKWRFQMSYTNSYCRGVQCVCFSKDDKYVYAGLKDRSIIVWSVLDGTLLAVQFVHAVVNRIIPTSNGFIAPTRHGYLIRERFQCPSSRVSQQDPLKNFKKAVWKVKSRQREELAAAAGALQDSRSASAQGNERKSNKRSQVCLLV

Target ORF information:

RefSeq Version	XM_022406917.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris NACHT and WD repeat domain containing 1 (NWD1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022406917.1

ORF Insert Sequence:

ATGAGTGTGG GCCCCCTCAG CCCGGGCACC CGCCAGCGAC TTCTCCAGGG TCAGGCAGAC 
CCCCTCCCAG CGCCTCTGTC CAGAAGTGTG GCCATCTTCA TCAGCTCCAC GGTCTCGGAT 
ATGGATGCAG AGAGAGAAGC TCTGCAGAGC ACTGCCTTCC CCGAAGTGCA GACCTTCTGC 
CAAAAGAATG GCCTGATGTT CGAGGTTGTT GACCTGAGGT GGGGTATTCG GAACACTGAG 
GCCACTGACC ACATGACCAC AGAACTCTGC TTGGAGGAGC TCGACCGGTG TCGGAAAACA 
TCCATAGGGC CGGCTTTTGT TGCCCTCTTA GGTGACCAGT ACGGCCCCTG TCCAGTCCCT 
ACACTGATCG AGGAGAAGGA GTGGGAGGCC CTGAGATCCC AGCTGGCCTC CAAGCCACGT 
GACCTGGAGC TGGTGGCTCG ACGCTTCCGG AGGGATGAGA ACGCCGTGCC CCCCACCTAC 
CTGCTTCAGG CCCTAGGCAC TGCGGAGACC CAGGGGCCCG AAGAGGCCAC CCTGACCTCT 
GTGCTGCGCT CCGGAGCCCA GGAGGCCCGG AGGCTGGGCC TCATCACCCA GGAGCAGTGG 
CACCGCTACC ACTGGTCAGT CATTGAGTGG GAGATAGACC GGGGCCTGCT GAGCTTGACA 
GACGGAGACC AGGGGGCGAC TGTCTTCTTG AGAGAGATCC AAGACCTCAA CAAACACATC 
CTGGAAGACT GCGCCCTCAA GATGGTGGAC CGGCTCGCAG ATGGCTGCCT GGACACAGAT 
GCCCAGAACC TTCTCAGTGG CCTCAAGGGA CGCATTGCCG ACACACAACC TGGAGTCCTC 
AAGGCCCACC ACCTGCCATG GAGCCGAGAC CTGGTGAACC CCAAAAACAA GGCTCATGCT 
CGGTACCTGA AAGAGCTGGG GGAGCAGTTT GTGGCCAGGG CTAACCACCA GGTCCTTGAG 
CACCTCCGGG AGCAGGAGGC AGGCAGGCAG GAGTTGGCGT GGCTCTACCA GGAGATCCGC 
CACCACCTGG GGCTGAGCAC CGAGGCCACC TGGACCTTTT GTGGCCGCCA GGAGCTCCTG 
GCCCAGCTGG GGCAGCGGCT CCGGCAGAGC GATGGCCATC CGCACTCACC CCTGGTGCTT 
TTTGGACCTC CAGGCATCGG AAAGACAGCG CTGATGTGCA AGTTGGCAGA ACAAATGCCG 
GGACTGCTTG GCCGCAAGAC AGTGACCGTC CTGCGGCTTC TGGGGATGTC ACAGATGAGC 
TCCGACGCCC GAGGCCTGCT CCAGAGCATC TGCTTCCAGG TGTGCCTGGC CTACGGGCTG 
CCCCTGCCCC CTGCCCAGGT CCTGGAGGCC CATGCCAGGG TGGTCCAGTT TTTCCACACC 
CTCCTCCACA CTGTCTCCTG CCGAAACTTC GAGTCACTTG TGATCCTGTT GGACTCCATG 
GATGATGTGG ATTCCATCTG CCGCGCCCGG AGGGTCCCCT GGCTGCCTCC CCGGTGCCCC 
CCGAGGATCC ACCTTATCCT CTCAGCCTGC TCGGGGAAGC CGGGGATTCT AGACATCATG 
AGACAGACGC TGACGGACCC AGAGGCCTAC TGGGAGGTGA AACCCCTCTC CGGAAACCAA 
GGGCAGGAGA TGATCCAGCT CCTGCTGGCC GCCTCGCGGA GGACGCTGAG CCCAGGGCAG 
AGGGAGCTGC TCTGGGCCAG CCTCCCAGAG TGTGGGCACC CAGGACGGCT GAGGCTGGCC 
TTTGAGGAGG CCCGGAAGTG GGCCTCCTTC ACCATACCTG CCCCTCTGGC CTCCACTGCT 
AAGGAAGCGA TCCATCAGCT GTGTGCCCGC CTGGAGCAGA CACACGGGCA GCTCCTGGTG 
GCCCACGTGC TGAGCTACAT CGTGTCCTCC CGGCATGGGC TCTCAGAGGC AGAGCTGAAG 
GACGTCCTGT CCTTGGACGA CGAGGTCCTG GAGGCGGTGT ACCAGGACTG GACCCCGCCC 
AGCAAGGAGC TGTTGCGCTT CCCCCCGTTG CTGTGGGTGA GGCTCCGTCG GGATCTCGGC 
CACTGCTTGG CCCGGCGGCC GGTGGACGGC TTCACGCTCC TGGCCATCGC CCACAGGCAG 
CTGACTGAGG TGATCCAAGA GCGCTACCTG TCTGGGCCCG AGAGAGCCAA GAGGCACGGA 
GTCTTGGCTG ACTTCTTCTC AGGGGCCTGG AGCCAGGGCA CAAAGAAACT CATCACCCTG 
CCACTCGTGG GGAAGCCCCT GAACCTGGAC CGCAAGGTGG CGCCTCAGCC CCTGTGGTTC 
TCAGACACAG TTGCAAATCT GCGGAAGCTG AAGGAGTTGC CCCATCACCT GGTCCACTCA 
GGCCGCTTCG AAGAGCTGAA GCAGGAGGTT CTGGGCAGCA TGAACTGGAT TTCCTGCCGG 
GGCGTCTCTG GGGGCATCGA GGGGCTGTTG GAGGACTTTG ACCTGTATGG CCCTCACCTG 
GATTGTCCCG AGGTCGGCCT GGTCCGGGAC GCCCTCCAGC TGTGCCGCCC GGCCCTGGAG 
CTCCGAGGCA TGGAGAGGAG CATCCTCTAC ACTGAACTCC TGGCCAGACT CCATTTCTTC 
GCCACCTCAC ATCCAGCGCT GGTGGGACAG CTGTGCCAAC AGGCCCAGAG CTGGTTCCGG 
CTGTGCCCCC ACCCTGTGCT GGTACCTCTC GGGGGGTTCC TCCAGCCCCC CGGGGGACCC 
CTCCGGGCAA CCTTCACTGG CTGTCACAAG GGCATCACTG CCATGGGCTG GAGCCTGGAA 
GAGAAGCTGC TGGTAGTTGG CACCCAGGAT GGCCTCATGG CTGTGTGGGA CATGGAAGAG 
CAGCAGGTGA TCCACATCCT AACTGGACAC ACAGGAGAGG TGAGGTCTGT GAAAGTGTTT 
GGCAAGGGGA CACTCGTCCT CTCTGCCTCA AAGGACCACA CACTGCGTTT GTGGAACTTA 
CTCTCTGGCC AGCAGAAATT CACCATTTGG GATGGAGGCT CAAAAGATCC CACTGACCCT 
GAGATCTGGA GTCTTCATGT GGATGAAGCA AAGAAGGTTG TGTATTTGGC ATCTGGCTCA 
AAGATCAGTG CTTGGAATCT GGAAACTGCA GAGCTGGTTT TCCATATCCT GGGAGATGCC 
TCCGACTGCT GGATGTGCAC AGCGGTGCTG GCTTCCCGGG CCACGCTGCT GACCGTGTCT 
GAGGGCGGTG TGGTCAGTCT GTGGAGCTCA GGCACAGGGA AACTGCAGGG GAGGCACCAT 
TTATCCAGCA TCAAAGAAGA AACACCTACC TGTGGGGTCT CGGTCCAGAA GCAAGGAAAG 
ATGGTGACTG GGTTCAGCAA AGGCTCCATC TCTTTGGTTT CCCCCGGAGG AGATAGCCTG 
CTGGAGAAGC TTCCAGAAGC CGTGAGGTTC CTGGTAGTCT CTGAAGATGA GTCCCTTCTC 
GCTGCAGGGT TTGGCAGATC CGTGCGGATA TTCTTGGCAG ACTCGCAGGG GTTTCATCGA 
TTTATGGCCA CCGACCTTGA ACATGAGGAC ACCGTGGAGA TAGCTGGTTT TGGTCCTGAG 
AACAATCTGA TTGTCACTGC GTCCCGCGAT GCACTCATCC AGGTGTGGAG CCTGTCGGAG 
CAGGGGACCC TGCTGGACAT CCTGGAAGGT GTCGGAGCCC CCGTGAGCCT GCTGGCCTGG 
GCTGGGGCCC TGGTGGCATC TGCTTCTCAG CAGTCCTCAT CTTTGAAAGT CTGGGATCTC 
ACTTACATTC AGAAGCCGAG GGCCTGCACC CCATTTCTGG ATCGTACTGG CCTCACTGCT 
GTGTCACACA ATGGAAGCTA CGTTTACTTC CCCAAAATCG GAGACAAGAA CAAAGTTACC 
ATTTGGGACT CTGCAGAAGG GGTTGTCCTG GTGTTTCCCC TGAATTCCAG ACAGGATGTG 
ATGTGCATCC CCCCTCCCGA GGCCCGGAAA GCCATCAACT GCATGGCCCT CAGTAAAGGC 
GAGGAGCGCC TGGCCATCGC CTATGACAGC ATTGTCCTTG TGCTGGACAT CAGTCCCGGG 
GATCCCTGTC CGGTTATTGA TGGGCCAACA TACACCTTCT ATACCCAGCT GCCTGAGGCC 
ATCTCCAGTG TGGCCGTTCT GGCTGACTAC CGCGTGATCT ACGGCATGAC CAACGGTGAC 
CTCTTTCTTT ACGAGTGTGC GAATTCCAAA GTGTTCCCTC TGGAGGCCCA CGGGAGCCAG 
GTCACCTGCA TGCAGGTCAG CCACAAGGAG CAGCTGGCTG TCAGCGGGTC CGAGGAAGCC 
CTGCTGTGTC TCTGGGACCT GCAGGCCTGC AAGTGGAGGT TCCAGATGAG CTACACGAAT 
TCTTACTGTA GAGGAGTCCA GTGTGTCTGC TTCTCCAAGG ATGACAAGTA TGTGTATGCG 
GGCTTGAAGG ATCGCTCCAT CATTGTCTGG AGTGTGCTGG ATGGCACCCT GCTGGCTGTC 
CAGTTTGTCC ATGCTGTGGT GAACAGGATC ATCCCAACTT CGAATGGCTT CATTGCCCCC 
ACTAGACATG GCTACCTGAT CCGCGAGCGT TTCCAGTGCC CTTCATCGAG AGTCTCACAG 
CAAGATCCTC TCAAGAACTT CAAGAAGGCA GTGTGGAAGG TCAAATCCAG GCAGAGAGAA 
GAGCTGGCAG CAGCTGCAGG AGCACTCCAG GACTCGAGAT CGGCAAGTGC CCAGGGAAAT 
GAACGTAAAT CGAACAAACG CTCACAAGTC TGCCTGCTAG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NWD1 ( XM_533883.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_533883.5 Canis lupus familiaris NACHT and WD repeat domain containing 1 (NWD1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe41720
Clone ID Related Accession (Same CDS sequence)	XM_533883.5
Accession Version	XM_533883.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4698bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	NACHT domain- and WD repeat-containing protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726096.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_533883.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCAG AGAGAGAAGC TCTGCAGAGC ACTGCCTTCC CCGAAGTGCA GACCTTCTGC 
CAAAAGAATG GCCTGATGTT CGAGGTTGTT GACCTGAGGT GGGGTATTCG GAACACTGAG 
GCCACTGACC ACATGACCAC AGAACTCTGC TTGGAGGAGC TCGACCGGTG TCGGAAAACA 
TCCATAGGGC CGGCTTTTGT TGCCCTCTTA GGTGACCAGT ACGGCCCCTG TCCAGTCCCT 
ACACTGATCG AGGAGAAGGA GTGGGAGGCC CTGAGATCCC AGCTGGCCTC CAAGCCACGT 
GACCTGGAGC TGGTGGCTCG ACGCTTCCGG AGGGATGAGA ACGCCGTGCC CCCCACCTAC 
CTGCTTCAGG CCCTAGGCAC TGCGGAGACC CAGGGGCCCG AAGAGGCCAC CCTGACCTCT 
GTGCTGCGCT CCGGAGCCCA GGAGGCCCGG AGGCTGGGCC TCATCACCCA GGAGCAGTGG 
CACCGCTACC ACTGGTCAGT CATTGAGTGG GAGATAGACC GGGGCCTGCT GAGCTTGACA 
GACGGAGACC AGGGGGCGAC TGTCTTCTTG AGAGAGATCC AAGACCTCAA CAAACACATC 
CTGGAAGACT GCGCCCTCAA GATGGTGGAC CGGCTCGCAG ATGGCTGCCT GGACACAGAT 
GCCCAGAACC TTCTCAGTGG CCTCAAGGGA CGCATTGCCG ACACACAACC TGGAGTCCTC 
AAGGCCCACC ACCTGCCATG GAGCCGAGAC CTGGTGAACC CCAAAAACAA GGCTCATGCT 
CGGTACCTGA AAGAGCTGGG GGAGCAGTTT GTGGCCAGGG CTAACCACCA GGTCCTTGAG 
CACCTCCGGG AGCAGGAGGC AGGCAGGCAG GAGTTGGCGT GGCTCTACCA GGAGATCCGC 
CACCACCTGG GGCTGAGCAC CGAGGCCACC TGGACCTTTT GTGGCCGCCA GGAGCTCCTG 
GCCCAGCTGG GGCAGCGGCT CCGGCAGAGC GATGGCCATC CGCACTCACC CCTGGTGCTT 
TTTGGACCTC CAGGCATCGG AAAGACAGCG CTGATGTGCA AGTTGGCAGA ACAAATGCCG 
GGACTGCTTG GCCGCAAGAC AGTGACCGTC CTGCGGCTTC TGGGGATGTC ACAGATGAGC 
TCCGACGCCC GAGGCCTGCT CCAGAGCATC TGCTTCCAGG TGTGCCTGGC CTACGGGCTG 
CCCCTGCCCC CTGCCCAGGT CCTGGAGGCC CATGCCAGGG TGGTCCAGTT TTTCCACACC 
CTCCTCCACA CTGTCTCCTG CCGAAACTTC GAGTCACTTG TGATCCTGTT GGACTCCATG 
GATGATGTGG ATTCCATCTG CCGCGCCCGG AGGGTCCCCT GGCTGCCTCC CCGGTGCCCC 
CCGAGGATCC ACCTTATCCT CTCAGCCTGC TCGGGGAAGC CGGGGATTCT AGACATCATG 
AGACAGACGC TGACGGACCC AGAGGCCTAC TGGGAGGTGA AACCCCTCTC CGGAAACCAA 
GGGCAGGAGA TGATCCAGCT CCTGCTGGCC GCCTCGCGGA GGACGCTGAG CCCAGGGCAG 
AGGGAGCTGC TCTGGGCCAG CCTCCCAGAG TGTGGGCACC CAGGACGGCT GAGGCTGGCC 
TTTGAGGAGG CCCGGAAGTG GGCCTCCTTC ACCATACCTG CCCCTCTGGC CTCCACTGCT 
AAGGAAGCGA TCCATCAGCT GTGTGCCCGC CTGGAGCAGA CACACGGGCA GCTCCTGGTG 
GCCCACGTGC TGAGCTACAT CGTGTCCTCC CGGCATGGGC TCTCAGAGGC AGAGCTGAAG 
GACGTCCTGT CCTTGGACGA CGAGGTCCTG GAGGCGGTGT ACCAGGACTG GACCCCGCCC 
AGCAAGGAGC TGTTGCGCTT CCCCCCGTTG CTGTGGGTGA GGCTCCGTCG GGATCTCGGC 
CACTGCTTGG CCCGGCGGCC GGTGGACGGC TTCACGCTCC TGGCCATCGC CCACAGGCAG 
CTGACTGAGG TGATCCAAGA GCGCTACCTG TCTGGGCCCG AGAGAGCCAA GAGGCACGGA 
GTCTTGGCTG ACTTCTTCTC AGGGGCCTGG AGCCAGGGCA CAAAGAAACT CATCACCCTG 
CCACTCGTGG GGAAGCCCCT GAACCTGGAC CGCAAGGTGG CGCCTCAGCC CCTGTGGTTC 
TCAGACACAG TTGCAAATCT GCGGAAGCTG AAGGAGTTGC CCCATCACCT GGTCCACTCA 
GGCCGCTTCG AAGAGCTGAA GCAGGAGGTT CTGGGCAGCA TGAACTGGAT TTCCTGCCGG 
GGCGTCTCTG GGGGCATCGA GGGGCTGTTG GAGGACTTTG ACCTGTATGG CCCTCACCTG 
GATTGTCCCG AGGTCGGCCT GGTCCGGGAC GCCCTCCAGC TGTGCCGCCC GGCCCTGGAG 
CTCCGAGGCA TGGAGAGGAG CATCCTCTAC ACTGAACTCC TGGCCAGACT CCATTTCTTC 
GCCACCTCAC ATCCAGCGCT GGTGGGACAG CTGTGCCAAC AGGCCCAGAG CTGGTTCCGG 
CTGTGCCCCC ACCCTGTGCT GGTACCTCTC GGGGGGTTCC TCCAGCCCCC CGGGGGACCC 
CTCCGGGCAA CCTTCACTGG CTGTCACAAG GGCATCACTG CCATGGGCTG GAGCCTGGAA 
GAGAAGCTGC TGGTAGTTGG CACCCAGGAT GGCCTCATGG CTGTGTGGGA CATGGAAGAG 
CAGCAGGTGA TCCACATCCT AACTGGACAC ACAGGAGAGG TGAGGTCTGT GAAAGTGTTT 
GGCAAGGGGA CACTCGTCCT CTCTGCCTCA AAGGACCACA CACTGCGTTT GTGGAACTTA 
CTCTCTGGCC AGCAGAAATT CACCATTTGG GATGGAGGCT CAAAAGATCC CACTGACCCT 
GAGATCTGGA GTCTTCATGT GGATGAAGCA AAGAAGGTTG TGTATTTGGC ATCTGGCTCA 
AAGATCAGTG CTTGGAATCT GGAAACTGCA GAGCTGGTTT TCCATATCCT GGGAGATGCC 
TCCGACTGCT GGATGTGCAC AGCGGTGCTG GCTTCCCGGG CCACGCTGCT GACCGTGTCT 
GAGGGCGGTG TGGTCAGTCT GTGGAGCTCA GGCACAGGGA AACTGCAGGG GAGGCACCAT 
TTATCCAGCA TCAAAGAAGA AACACCTACC TGTGGGGTCT CGGTCCAGAA GCAAGGAAAG 
ATGGTGACTG GGTTCAGCAA AGGCTCCATC TCTTTGGTTT CCCCCGGAGG AGATAGCCTG 
CTGGAGAAGC TTCCAGAAGC CGTGAGGTTC CTGGTAGTCT CTGAAGATGA GTCCCTTCTC 
GCTGCAGGGT TTGGCAGATC CGTGCGGATA TTCTTGGCAG ACTCGCAGGG GTTTCATCGA 
TTTATGGCCA CCGACCTTGA ACATGAGGAC ACCGTGGAGA TAGCTGGTTT TGGTCCTGAG 
AACAATCTGA TTGTCACTGC GTCCCGCGAT GCACTCATCC AGGTGTGGAG CCTGTCGGAG 
CAGGGGACCC TGCTGGACAT CCTGGAAGGT GTCGGAGCCC CCGTGAGCCT GCTGGCCTGG 
GCTGGGGCCC TGGTGGCATC TGCTTCTCAG CAGTCCTCAT CTTTGAAAGT CTGGGATCTC 
ACTTACATTC AGAAGCCGAG GGCCTGCACC CCATTTCTGG ATCGTACTGG CCTCACTGCT 
GTGTCACACA ATGGAAGCTA CGTTTACTTC CCCAAAATCG GAGACAAGAA CAAAGTTACC 
ATTTGGGACT CTGCAGAAGG CGAGGAACAA GATGCCCTGG ACACCTCCAA TGAGGTCAGG 
TGTCTGGAGG TTGCCCAGCA GGCCAATCTC CTTTTCACCG GCCTCGTTTC AGGGGTTGTC 
CTGGTGTTTC CCCTGAATTC CAGACAGGAT GTGATGTGCA TCCCCCCTCC CGAGGCCCGG 
AAAGCCATCA ACTGCATGGC CCTCAGTAAA GGCGAGGAGC GCCTGGCCAT CGCCTATGAC 
AGCATTGTCC TTGTGCTGGA CATCAGTCCC GGGGATCCCT GTCCGGTTAT TGATGGGCCA 
ACATACACCT TCTATACCCA GCTGCCTGAG GCCATCTCCA GTGTGGCCGT TCTGGCTGAC 
TACCGCGTGA TCTACGGCAT GACCAACGGT GACCTCTTTC TTTACGAGTG TGCGAATTCC 
AAAGTGTTCC CTCTGGAGGC CCACGGGAGC CAGGTCACCT GCATGCAGGT CAGCCACAAG 
GAGCAGCTGG CTGTCAGCGG GTCCGAGGAA GCCCTGCTGT GTCTCTGGGA CCTGCAGGCC 
TGCAAGTGGA GGTTCCAGAT GAGCTACACG AATTCTTACT GTAGAGGAGT CCAGTGTGTC 
TGCTTCTCCA AGGATGACAA GTATGTGTAT GCGGGCTTGA AGGATCGCTC CATCATTGTC 
TGGAGTGTGC TGGATGGCAC CCTGCTGGCT GTCCAGTTTG TCCATGCTGT GGTGAACAGG 
ATCATCCCAA CTTCGAATGG CTTCATTGCC CCCACTAGAC ATGGCTACCT GATCCGCGAG 
CGTTTCCAGT GCCCTTCATC GAGAGTCTCA CAGCAAGATC CTCTCAAGAA CTTCAAGAAG 
GCAGTGTGGA AGGTCAAATC CAGGCAGAGA GAAGAGCTGG CAGCAGCTGC AGGAGCACTC 
CAGGACTCGA GATCGGCAAG TGCCCAGGGA AATGAACGTA AATCGAACAA ACGCTCACAA 
GTCTGCCTGC TAGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_533883.5
CDS	522..5219
Translation	MDAEREALQSTAFPEVQTFCQKNGLMFEVVDLRWGIRNTEATDHMTTELCLEELDRCRKTSIGPAFVALLGDQYGPCPVPTLIEEKEWEALRSQLASKPRDLELVARRFRRDENAVPPTYLLQALGTAETQGPEEATLTSVLRSGAQEARRLGLITQEQWHRYHWSVIEWEIDRGLLSLTDGDQGATVFLREIQDLNKHILEDCALKMVDRLADGCLDTDAQNLLSGLKGRIADTQPGVLKAHHLPWSRDLVNPKNKAHARYLKELGEQFVARANHQVLEHLREQEAGRQELAWLYQEIRHHLGLSTEATWTFCGRQELLAQLGQRLRQSDGHPHSPLVLFGPPGIGKTALMCKLAEQMPGLLGRKTVTVLRLLGMSQMSSDARGLLQSICFQVCLAYGLPLPPAQVLEAHARVVQFFHTLLHTVSCRNFESLVILLDSMDDVDSICRARRVPWLPPRCPPRIHLILSACSGKPGILDIMRQTLTDPEAYWEVKPLSGNQGQEMIQLLLAASRRTLSPGQRELLWASLPECGHPGRLRLAFEEARKWASFTIPAPLASTAKEAIHQLCARLEQTHGQLLVAHVLSYIVSSRHGLSEAELKDVLSLDDEVLEAVYQDWTPPSKELLRFPPLLWVRLRRDLGHCLARRPVDGFTLLAIAHRQLTEVIQERYLSGPERAKRHGVLADFFSGAWSQGTKKLITLPLVGKPLNLDRKVAPQPLWFSDTVANLRKLKELPHHLVHSGRFEELKQEVLGSMNWISCRGVSGGIEGLLEDFDLYGPHLDCPEVGLVRDALQLCRPALELRGMERSILYTELLARLHFFATSHPALVGQLCQQAQSWFRLCPHPVLVPLGGFLQPPGGPLRATFTGCHKGITAMGWSLEEKLLVVGTQDGLMAVWDMEEQQVIHILTGHTGEVRSVKVFGKGTLVLSASKDHTLRLWNLLSGQQKFTIWDGGSKDPTDPEIWSLHVDEAKKVVYLASGSKISAWNLETAELVFHILGDASDCWMCTAVLASRATLLTVSEGGVVSLWSSGTGKLQGRHHLSSIKEETPTCGVSVQKQGKMVTGFSKGSISLVSPGGDSLLEKLPEAVRFLVVSEDESLLAAGFGRSVRIFLADSQGFHRFMATDLEHEDTVEIAGFGPENNLIVTASRDALIQVWSLSEQGTLLDILEGVGAPVSLLAWAGALVASASQQSSSLKVWDLTYIQKPRACTPFLDRTGLTAVSHNGSYVYFPKIGDKNKVTIWDSAEGEEQDALDTSNEVRCLEVAQQANLLFTGLVSGVVLVFPLNSRQDVMCIPPPEARKAINCMALSKGEERLAIAYDSIVLVLDISPGDPCPVIDGPTYTFYTQLPEAISSVAVLADYRVIYGMTNGDLFLYECANSKVFPLEAHGSQVTCMQVSHKEQLAVSGSEEALLCLWDLQACKWRFQMSYTNSYCRGVQCVCFSKDDKYVYAGLKDRSIIVWSVLDGTLLAVQFVHAVVNRIIPTSNGFIAPTRHGYLIRERFQCPSSRVSQQDPLKNFKKAVWKVKSRQREELAAAAGALQDSRSASAQGNERKSNKRSQVCLLV

Target ORF information:

RefSeq Version	XM_533883.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris NACHT and WD repeat domain containing 1 (NWD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_533883.5

ORF Insert Sequence:

ATGGATGCAG AGAGAGAAGC TCTGCAGAGC ACTGCCTTCC CCGAAGTGCA GACCTTCTGC 
CAAAAGAATG GCCTGATGTT CGAGGTTGTT GACCTGAGGT GGGGTATTCG GAACACTGAG 
GCCACTGACC ACATGACCAC AGAACTCTGC TTGGAGGAGC TCGACCGGTG TCGGAAAACA 
TCCATAGGGC CGGCTTTTGT TGCCCTCTTA GGTGACCAGT ACGGCCCCTG TCCAGTCCCT 
ACACTGATCG AGGAGAAGGA GTGGGAGGCC CTGAGATCCC AGCTGGCCTC CAAGCCACGT 
GACCTGGAGC TGGTGGCTCG ACGCTTCCGG AGGGATGAGA ACGCCGTGCC CCCCACCTAC 
CTGCTTCAGG CCCTAGGCAC TGCGGAGACC CAGGGGCCCG AAGAGGCCAC CCTGACCTCT 
GTGCTGCGCT CCGGAGCCCA GGAGGCCCGG AGGCTGGGCC TCATCACCCA GGAGCAGTGG 
CACCGCTACC ACTGGTCAGT CATTGAGTGG GAGATAGACC GGGGCCTGCT GAGCTTGACA 
GACGGAGACC AGGGGGCGAC TGTCTTCTTG AGAGAGATCC AAGACCTCAA CAAACACATC 
CTGGAAGACT GCGCCCTCAA GATGGTGGAC CGGCTCGCAG ATGGCTGCCT GGACACAGAT 
GCCCAGAACC TTCTCAGTGG CCTCAAGGGA CGCATTGCCG ACACACAACC TGGAGTCCTC 
AAGGCCCACC ACCTGCCATG GAGCCGAGAC CTGGTGAACC CCAAAAACAA GGCTCATGCT 
CGGTACCTGA AAGAGCTGGG GGAGCAGTTT GTGGCCAGGG CTAACCACCA GGTCCTTGAG 
CACCTCCGGG AGCAGGAGGC AGGCAGGCAG GAGTTGGCGT GGCTCTACCA GGAGATCCGC 
CACCACCTGG GGCTGAGCAC CGAGGCCACC TGGACCTTTT GTGGCCGCCA GGAGCTCCTG 
GCCCAGCTGG GGCAGCGGCT CCGGCAGAGC GATGGCCATC CGCACTCACC CCTGGTGCTT 
TTTGGACCTC CAGGCATCGG AAAGACAGCG CTGATGTGCA AGTTGGCAGA ACAAATGCCG 
GGACTGCTTG GCCGCAAGAC AGTGACCGTC CTGCGGCTTC TGGGGATGTC ACAGATGAGC 
TCCGACGCCC GAGGCCTGCT CCAGAGCATC TGCTTCCAGG TGTGCCTGGC CTACGGGCTG 
CCCCTGCCCC CTGCCCAGGT CCTGGAGGCC CATGCCAGGG TGGTCCAGTT TTTCCACACC 
CTCCTCCACA CTGTCTCCTG CCGAAACTTC GAGTCACTTG TGATCCTGTT GGACTCCATG 
GATGATGTGG ATTCCATCTG CCGCGCCCGG AGGGTCCCCT GGCTGCCTCC CCGGTGCCCC 
CCGAGGATCC ACCTTATCCT CTCAGCCTGC TCGGGGAAGC CGGGGATTCT AGACATCATG 
AGACAGACGC TGACGGACCC AGAGGCCTAC TGGGAGGTGA AACCCCTCTC CGGAAACCAA 
GGGCAGGAGA TGATCCAGCT CCTGCTGGCC GCCTCGCGGA GGACGCTGAG CCCAGGGCAG 
AGGGAGCTGC TCTGGGCCAG CCTCCCAGAG TGTGGGCACC CAGGACGGCT GAGGCTGGCC 
TTTGAGGAGG CCCGGAAGTG GGCCTCCTTC ACCATACCTG CCCCTCTGGC CTCCACTGCT 
AAGGAAGCGA TCCATCAGCT GTGTGCCCGC CTGGAGCAGA CACACGGGCA GCTCCTGGTG 
GCCCACGTGC TGAGCTACAT CGTGTCCTCC CGGCATGGGC TCTCAGAGGC AGAGCTGAAG 
GACGTCCTGT CCTTGGACGA CGAGGTCCTG GAGGCGGTGT ACCAGGACTG GACCCCGCCC 
AGCAAGGAGC TGTTGCGCTT CCCCCCGTTG CTGTGGGTGA GGCTCCGTCG GGATCTCGGC 
CACTGCTTGG CCCGGCGGCC GGTGGACGGC TTCACGCTCC TGGCCATCGC CCACAGGCAG 
CTGACTGAGG TGATCCAAGA GCGCTACCTG TCTGGGCCCG AGAGAGCCAA GAGGCACGGA 
GTCTTGGCTG ACTTCTTCTC AGGGGCCTGG AGCCAGGGCA CAAAGAAACT CATCACCCTG 
CCACTCGTGG GGAAGCCCCT GAACCTGGAC CGCAAGGTGG CGCCTCAGCC CCTGTGGTTC 
TCAGACACAG TTGCAAATCT GCGGAAGCTG AAGGAGTTGC CCCATCACCT GGTCCACTCA 
GGCCGCTTCG AAGAGCTGAA GCAGGAGGTT CTGGGCAGCA TGAACTGGAT TTCCTGCCGG 
GGCGTCTCTG GGGGCATCGA GGGGCTGTTG GAGGACTTTG ACCTGTATGG CCCTCACCTG 
GATTGTCCCG AGGTCGGCCT GGTCCGGGAC GCCCTCCAGC TGTGCCGCCC GGCCCTGGAG 
CTCCGAGGCA TGGAGAGGAG CATCCTCTAC ACTGAACTCC TGGCCAGACT CCATTTCTTC 
GCCACCTCAC ATCCAGCGCT GGTGGGACAG CTGTGCCAAC AGGCCCAGAG CTGGTTCCGG 
CTGTGCCCCC ACCCTGTGCT GGTACCTCTC GGGGGGTTCC TCCAGCCCCC CGGGGGACCC 
CTCCGGGCAA CCTTCACTGG CTGTCACAAG GGCATCACTG CCATGGGCTG GAGCCTGGAA 
GAGAAGCTGC TGGTAGTTGG CACCCAGGAT GGCCTCATGG CTGTGTGGGA CATGGAAGAG 
CAGCAGGTGA TCCACATCCT AACTGGACAC ACAGGAGAGG TGAGGTCTGT GAAAGTGTTT 
GGCAAGGGGA CACTCGTCCT CTCTGCCTCA AAGGACCACA CACTGCGTTT GTGGAACTTA 
CTCTCTGGCC AGCAGAAATT CACCATTTGG GATGGAGGCT CAAAAGATCC CACTGACCCT 
GAGATCTGGA GTCTTCATGT GGATGAAGCA AAGAAGGTTG TGTATTTGGC ATCTGGCTCA 
AAGATCAGTG CTTGGAATCT GGAAACTGCA GAGCTGGTTT TCCATATCCT GGGAGATGCC 
TCCGACTGCT GGATGTGCAC AGCGGTGCTG GCTTCCCGGG CCACGCTGCT GACCGTGTCT 
GAGGGCGGTG TGGTCAGTCT GTGGAGCTCA GGCACAGGGA AACTGCAGGG GAGGCACCAT 
TTATCCAGCA TCAAAGAAGA AACACCTACC TGTGGGGTCT CGGTCCAGAA GCAAGGAAAG 
ATGGTGACTG GGTTCAGCAA AGGCTCCATC TCTTTGGTTT CCCCCGGAGG AGATAGCCTG 
CTGGAGAAGC TTCCAGAAGC CGTGAGGTTC CTGGTAGTCT CTGAAGATGA GTCCCTTCTC 
GCTGCAGGGT TTGGCAGATC CGTGCGGATA TTCTTGGCAG ACTCGCAGGG GTTTCATCGA 
TTTATGGCCA CCGACCTTGA ACATGAGGAC ACCGTGGAGA TAGCTGGTTT TGGTCCTGAG 
AACAATCTGA TTGTCACTGC GTCCCGCGAT GCACTCATCC AGGTGTGGAG CCTGTCGGAG 
CAGGGGACCC TGCTGGACAT CCTGGAAGGT GTCGGAGCCC CCGTGAGCCT GCTGGCCTGG 
GCTGGGGCCC TGGTGGCATC TGCTTCTCAG CAGTCCTCAT CTTTGAAAGT CTGGGATCTC 
ACTTACATTC AGAAGCCGAG GGCCTGCACC CCATTTCTGG ATCGTACTGG CCTCACTGCT 
GTGTCACACA ATGGAAGCTA CGTTTACTTC CCCAAAATCG GAGACAAGAA CAAAGTTACC 
ATTTGGGACT CTGCAGAAGG CGAGGAACAA GATGCCCTGG ACACCTCCAA TGAGGTCAGG 
TGTCTGGAGG TTGCCCAGCA GGCCAATCTC CTTTTCACCG GCCTCGTTTC AGGGGTTGTC 
CTGGTGTTTC CCCTGAATTC CAGACAGGAT GTGATGTGCA TCCCCCCTCC CGAGGCCCGG 
AAAGCCATCA ACTGCATGGC CCTCAGTAAA GGCGAGGAGC GCCTGGCCAT CGCCTATGAC 
AGCATTGTCC TTGTGCTGGA CATCAGTCCC GGGGATCCCT GTCCGGTTAT TGATGGGCCA 
ACATACACCT TCTATACCCA GCTGCCTGAG GCCATCTCCA GTGTGGCCGT TCTGGCTGAC 
TACCGCGTGA TCTACGGCAT GACCAACGGT GACCTCTTTC TTTACGAGTG TGCGAATTCC 
AAAGTGTTCC CTCTGGAGGC CCACGGGAGC CAGGTCACCT GCATGCAGGT CAGCCACAAG 
GAGCAGCTGG CTGTCAGCGG GTCCGAGGAA GCCCTGCTGT GTCTCTGGGA CCTGCAGGCC 
TGCAAGTGGA GGTTCCAGAT GAGCTACACG AATTCTTACT GTAGAGGAGT CCAGTGTGTC 
TGCTTCTCCA AGGATGACAA GTATGTGTAT GCGGGCTTGA AGGATCGCTC CATCATTGTC 
TGGAGTGTGC TGGATGGCAC CCTGCTGGCT GTCCAGTTTG TCCATGCTGT GGTGAACAGG 
ATCATCCCAA CTTCGAATGG CTTCATTGCC CCCACTAGAC ATGGCTACCT GATCCGCGAG 
CGTTTCCAGT GCCCTTCATC GAGAGTCTCA CAGCAAGATC CTCTCAAGAA CTTCAAGAAG 
GCAGTGTGGA AGGTCAAATC CAGGCAGAGA GAAGAGCTGG CAGCAGCTGC AGGAGCACTC 
CAGGACTCGA GATCGGCAAG TGCCCAGGGA AATGAACGTA AATCGAACAA ACGCTCACAA 
GTCTGCCTGC TAGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NWD1 ( XM_014121993.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013977468.1 Canis lupus familiaris NACHT and WD repeat domain containing 1 (NWD1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe41721
Clone ID Related Accession (Same CDS sequence)	XM_014121993.1
Accession Version	XM_014121993.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	NACHT domain- and WD repeat-containing protein 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726096.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCAG AGAGAGAAGC TCTGCAGAGC ACTGCCTTCC CCGAAGTGCA GACCTTCTGC 
CAAAAGAATG GCCTGATGTT CGAGGTTGTT GACCTGAGGT GGGGTATTCG GAACACTGAG 
GCCACTGACC ACATGACCAC AGAACTCTGC TTGGAGGAGC TCGACCGGTG TCGGAAAACA 
TCCATAGGGC CGGCTTTTGT TGCCCTCTTA GGTGACCAGT ACGGCCCCTG TCCAGTCCCT 
ACACTGATCG AGGAGAAGGA GTGGGAGGCC CTGAGATCCC AGCTGGCCTC CAAGCCACGT 
GACCTGGAGC TGGTGGCTCG ACGCTTCCGG AGGGATGAGA ACGCCGTGCC CCCCACCTAC 
CTGCTTCAGG CCCTAGGCAC TGCGGAGACC CAGGGGCCCG AAGAGGCCAC CCTGACCTCT 
GTGCTGCGCT CCGGAGCCCA GGAGGCCCGG AGGCTGGGCC TCATCACCCA GGAGCAGTGG 
CACCGCTACC ACTGGTCAGT CATTGAGTGG GAGATAGACC GGGGCCTGCT GAGCTTGACA 
GACGGAGACC AGGGGGCGAC TGTCTTCTTG AGAGAGATCC AAGACCTCAA CAAACACATC 
CTGGAAGACT GCGCCCTCAA GATGGTGGAC CGGCTCGCAG ATGGCTGCCT GGACACAGAT 
GCCCAGAACC TTCTCAGTGG CCTCAAGGGA CGCATTGCCG ACACACAACC TGGAGTCCTC 
AAGGCCCACC ACCTGCCATG GAGCCGAGAC CTGGTGAACC CCAAAAACAA GGCTCATGCT 
CGGTACCTGA AAGAGCTGGG GGAGCAGTTT GTGGCCAGGG CTAACCACCA GGTCCTTGAG 
CACCTCCGGG AGCAGGAGGC AGGCAGGCAG GAGTTGGCGT GGCTCTACCA GGAGATCCGC 
CACCACCTGG GGCTGAGCAC CGAGGCCACC TGGACCTTTT GTGGCCGCCA GGAGCTCCTG 
GCCCAGCTGG GGCAGCGGCT CCGGCAGAGC GATGGCCATC CGCACTCACC CCTGGTGCTT 
TTTGGACCTC CAGGCATCGG AAAGACAGCG CTGATGTGCA AGTTGGCAGA ACAAATGCCG 
GGACTGCTTG GCCGCAAGAC AGTGACCGTC CTGCGGCTTC TGGGGATGTC ACAGATGAGC 
TCCGACGCCC GAGGCCTGCT CCAGAGCATC TGCTTCCAGG TGTGCCTGGC CTACGGGCTG 
CCCCTGCCCC CTGCCCAGGT CCTGGAGGCC CATGCCAGGG TGGTCCAGTT TTTCCACACC 
CTCCTCCACA CTGTCTCCTG CCGAAACTTC GAGTCACTTG TGATCCTGTT GGACTCCATG 
GATGATGTGG ATTCCATCTG CCGCGCCCGG AGGGTCCCCT GGCTGCCTCC CCGGTGCCCC 
CCGAGGATCC ACCTTATCCT CTCAGCCTGC TCGGGGAAGC CGGGGATTCT AGACATCATG 
AGACAGACGC TGACGGACCC AGAGGCCTAC TGGGAGGTGA AACCCCTCTC CGGAAACCAA 
GGGCAGGAGA TGATCCAGCT CCTGCTGGCC GCCTCGCGGA GGACGCTGAG CCCAGGGCAG 
AGGGAGCTGC TCTGGGCCAG CCTCCCAGAG TGTGGGCACC CAGGACGGCT GAGGCTGGCC 
TTTGAGGAGG CCCGGAAGTG GGCCTCCTTC ACCATACCTG CCCCTCTGGC CTCCACTGCT 
AAGGAAGCGA TCCATCAGCT GTGTGCCCGC CTGGAGCAGA CACACGGGCA GCTCCTGGTG 
GCCCACGTGC TGAGCTACAT CGTGTCCTCC CGGCATGGGC TCTCAGAGGC AGAGCTGAAG 
GACGTCCTGT CCTTGGACGA CGAGGTCCTG GAGGCGGTGT ACCAGGACTG GACCCCGCCC 
AGCAAGGAGC TGTTGCGCTT CCCCCCGTTG CTGTGGGTGA GGCTCCGTCG GGATCTCGGC 
CACTGCTTGG CCCGGCGGCC GGTGGACGGC TTCACGCTCC TGGCCATCGC CCACAGGCAG 
CTGACTGAGG TGATCCAAGA GCGCTACCTG TCTGGGCCCG AGAGAGCCAA GAGGCACGGA 
GTCTTGGCTG ACTTCTTCTC AGGGGCCTGG AGCCAGGGCA CAAAGAAACT CATCACCCTG 
CCACTCGTGG GGAAGCCCCT GAACCTGGAC CGCAAGGTGG CGCCTCAGCC CCTGTGGTTC 
TCAGACACAG TTGCAAATCT GCGGAAGCTG AAGGAGTTGC CCCATCACCT GGTCCACTCA 
GGCCGCTTCG AAGAGCTGAA GCAGGAGGTT CTGGGCAGCA TGAACTGGAT TTCCTGCCGG 
GGCGTCTCTG GGGGCATCGA GGGGCTGTTG GAGGACTTTG ACCTGTATGG CCCTCACCTG 
GATTGTCCCG AGGTCGGCCT GGTCCGGGAC GCCCTCCAGC TGTGCCGCCC GGCCCTGGAG 
CTCCGAGGCA TGGAGAGGAG CATCCTCTAC ACTGAACTCC TGGCCAGACT CCATTTCTTC 
GCCACCTCAC ATCCAGCGCT GGTGGGACAG CTGTGCCAAC AGGCCCAGAG CTGGTTCCGG 
CTGTGCCCCC ACCCTGTGCT GGTACCTCTC GGGGGGTTCC TCCAGCCCCC CGGGGGACCC 
CTCCGGGCAA CCTTCACTGG CTGTCACAAG GGCATCACTG CCATGGGCTG GAGCCTGGAA 
GAGAAGCTGC TGGTAGTTGG CACCCAGGAT GGCCTCATGG CTGTGTGGGA CATGGAAGAG 
CAGCAGGTGA TCCACATCCT AACTGGACAC ACAGGAGAGG TGAGGTCTGT GAAAGTGTTT 
GGCAAGGGGA CACTCGTCCT CTCTGCCTCA AAGGACCACA CACTGCGTTT GTGGAACTTA 
CTCTCTGGCC AGCAGAAATT CACCATTTGG GATGGAGGCT CAAAAGATCC CACTGACCCT 
GAGATCTGGA GTCTTCATGT GGATGAAGCA AAGAAGGTTG TGTATTTGGC ATCTGGCTCA 
AAGATCAGTG CTTGGAATCT GGAAACTGCA GAGCTGGTTT TCCATATCCT GGGAGATGCC 
TCCGACTGCT GGATGTGCAC AGCGGTGCTG GCTTCCCGGG CCACGCTGCT GACCGTGTCT 
GAGGGCGGTG TGGTCAGTCT GTGGAGCTCA GGCACAGGGA AACTGCAGGG GAGGCACCAT 
TTATCCAGCA TCAAAGAAGA AACACCTACC TGTGGGGTCT CGGTCCAGAA GCAAGGAAAG 
ATGGTGACTG GGTTCAGCAA AGGCTCCATC TCTTTGGTTT CCCCCGGAGG AGATAGCCTG 
CTGGAGAAGC TTCCAGAAGC CGTGAGGTTC CTGGTAGTCT CTGAAGATGA GTCCCTTCTC 
GCTGCAGGGT TTGGCAGATC CGTGCGGATA TTCTTGGCAG ACTCGCAGGG GTTTCATCGA 
TTTATGGCCA CCGACCTTGA ACATGAGGAC ACCGTGGAGA TAGCTGGTTT TGGTCCTGAG 
AACAATCTGA TTGTCACTGC GTCCCGCGAT GCACTCATCC AGGTGTGGAG CCTGTCGGAG 
CAGGGGACCC TGCTGGACAT CCTGGAAGGT GTCGGAGCCC CCGTGAGCCT GCTGGCCTGG 
GCTGGGGCCC TGGTGGCATC TGCTTCTCAG CAGTCCTCAT CTTTGAAAGT CTGGGATCTC 
ACTTACATTC AGAAGCCGAG GGCCTGCACC CCATTTCTGG ATCGTACTGG CCTCACTGCT 
GTGTCACACA ATGGAAGCTA CGTTTACTTC CCCAAAATCG GAGACAAGAA CAAAGTTACC 
ATTTGGGACT CTGCAGAAGG GGTTGTCCTG GTGTTTCCCC TGAATTCCAG ACAGGATGTG 
ATGTGCATCC CCCCTCCCGA GGCCCGGAAA GCCATCAACT GCATGGCCCT CAGTAAAGGC 
GAGGAGCGCC TGGCCATCGC CTATGACAGC ATTGTCCTTG TGCTGGACAT CAGTCCCGGG 
GATCCCTGTC CGGTTATTGA TGGGCCAACA TACACCTTCT ATACCCAGCT GCCTGAGGCC 
ATCTCCAGTG TGGCCGTTCT GGCTGACTAC CGCGTGATCT ACGGCATGAC CAACGGTGAC 
CTCTTTCTTT ACGAGTGTGC GAATTCCAAA GTGTTCCCTC TGGAGGCCCA CGGGAGCCAG 
GTCACCTGCA TGCAGGTCAG CCACAAGGAG CAGCTGGCTG TCAGCGGGTC CGAGGAAGCC 
CTGCTGTGTC TCTGGGACCT GCAGGCCTGC AAGTGGAGGT TCCAGATGAG CTACACGAAT 
TCTTACTGTA GAGGAGTCCA GTGTGTCTGC TTCTCCAAGG ATGACAAGTA TGTGTATGCG 
GGCTTGAAGG ATCGCTCCAT CATTGTCTGG AGTGTGCTGG ATGGCACCCT GCTGGCTGTC 
CAGTTTGTCC ATGCTGTGGT GAACAGGATC ATCCCAACTT CGAATGGCTT CATTGCCCCC 
ACTAGACATG GCTACCTGAT CCGCGAGCGT TTCCAGTGCC CTTCATCGAG AGTCTCACAG 
CAAGATCCTC TCAAGAACTT CAAGAAGGCA GTGTGGAAGG TCAAATCCAG GCAGAGAGAA 
GAGCTGGCAG CAGCTGCAGG AGCACTCCAG GACTCGAGAT CGGCAAGTGC CCAGGGAAAT 
GAACGTAAAT CGAACAAACG CTCACAAGTC TGCCTGCTAG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013977468.1
CDS	523..5127
Translation	MDAEREALQSTAFPEVQTFCQKNGLMFEVVDLRWGIRNTEATDHMTTELCLEELDRCRKTSIGPAFVALLGDQYGPCPVPTLIEEKEWEALRSQLASKPRDLELVARRFRRDENAVPPTYLLQALGTAETQGPEEATLTSVLRSGAQEARRLGLITQEQWHRYHWSVIEWEIDRGLLSLTDGDQGATVFLREIQDLNKHILEDCALKMVDRLADGCLDTDAQNLLSGLKGRIADTQPGVLKAHHLPWSRDLVNPKNKAHARYLKELGEQFVARANHQVLEHLREQEAGRQELAWLYQEIRHHLGLSTEATWTFCGRQELLAQLGQRLRQSDGHPHSPLVLFGPPGIGKTALMCKLAEQMPGLLGRKTVTVLRLLGMSQMSSDARGLLQSICFQVCLAYGLPLPPAQVLEAHARVVQFFHTLLHTVSCRNFESLVILLDSMDDVDSICRARRVPWLPPRCPPRIHLILSACSGKPGILDIMRQTLTDPEAYWEVKPLSGNQGQEMIQLLLAASRRTLSPGQRELLWASLPECGHPGRLRLAFEEARKWASFTIPAPLASTAKEAIHQLCARLEQTHGQLLVAHVLSYIVSSRHGLSEAELKDVLSLDDEVLEAVYQDWTPPSKELLRFPPLLWVRLRRDLGHCLARRPVDGFTLLAIAHRQLTEVIQERYLSGPERAKRHGVLADFFSGAWSQGTKKLITLPLVGKPLNLDRKVAPQPLWFSDTVANLRKLKELPHHLVHSGRFEELKQEVLGSMNWISCRGVSGGIEGLLEDFDLYGPHLDCPEVGLVRDALQLCRPALELRGMERSILYTELLARLHFFATSHPALVGQLCQQAQSWFRLCPHPVLVPLGGFLQPPGGPLRATFTGCHKGITAMGWSLEEKLLVVGTQDGLMAVWDMEEQQVIHILTGHTGEVRSVKVFGKGTLVLSASKDHTLRLWNLLSGQQKFTIWDGGSKDPTDPEIWSLHVDEAKKVVYLASGSKISAWNLETAELVFHILGDASDCWMCTAVLASRATLLTVSEGGVVSLWSSGTGKLQGRHHLSSIKEETPTCGVSVQKQGKMVTGFSKGSISLVSPGGDSLLEKLPEAVRFLVVSEDESLLAAGFGRSVRIFLADSQGFHRFMATDLEHEDTVEIAGFGPENNLIVTASRDALIQVWSLSEQGTLLDILEGVGAPVSLLAWAGALVASASQQSSSLKVWDLTYIQKPRACTPFLDRTGLTAVSHNGSYVYFPKIGDKNKVTIWDSAEGVVLVFPLNSRQDVMCIPPPEARKAINCMALSKGEERLAIAYDSIVLVLDISPGDPCPVIDGPTYTFYTQLPEAISSVAVLADYRVIYGMTNGDLFLYECANSKVFPLEAHGSQVTCMQVSHKEQLAVSGSEEALLCLWDLQACKWRFQMSYTNSYCRGVQCVCFSKDDKYVYAGLKDRSIIVWSVLDGTLLAVQFVHAVVNRIIPTSNGFIAPTRHGYLIRERFQCPSSRVSQQDPLKNFKKAVWKVKSRQREELAAAAGALQDSRSASAQGNERKSNKRSQVCLLV

Target ORF information:

RefSeq Version	XM_014121993.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris NACHT and WD repeat domain containing 1 (NWD1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121993.1

ORF Insert Sequence:

ATGGATGCAG AGAGAGAAGC TCTGCAGAGC ACTGCCTTCC CCGAAGTGCA GACCTTCTGC 
CAAAAGAATG GCCTGATGTT CGAGGTTGTT GACCTGAGGT GGGGTATTCG GAACACTGAG 
GCCACTGACC ACATGACCAC AGAACTCTGC TTGGAGGAGC TCGACCGGTG TCGGAAAACA 
TCCATAGGGC CGGCTTTTGT TGCCCTCTTA GGTGACCAGT ACGGCCCCTG TCCAGTCCCT 
ACACTGATCG AGGAGAAGGA GTGGGAGGCC CTGAGATCCC AGCTGGCCTC CAAGCCACGT 
GACCTGGAGC TGGTGGCTCG ACGCTTCCGG AGGGATGAGA ACGCCGTGCC CCCCACCTAC 
CTGCTTCAGG CCCTAGGCAC TGCGGAGACC CAGGGGCCCG AAGAGGCCAC CCTGACCTCT 
GTGCTGCGCT CCGGAGCCCA GGAGGCCCGG AGGCTGGGCC TCATCACCCA GGAGCAGTGG 
CACCGCTACC ACTGGTCAGT CATTGAGTGG GAGATAGACC GGGGCCTGCT GAGCTTGACA 
GACGGAGACC AGGGGGCGAC TGTCTTCTTG AGAGAGATCC AAGACCTCAA CAAACACATC 
CTGGAAGACT GCGCCCTCAA GATGGTGGAC CGGCTCGCAG ATGGCTGCCT GGACACAGAT 
GCCCAGAACC TTCTCAGTGG CCTCAAGGGA CGCATTGCCG ACACACAACC TGGAGTCCTC 
AAGGCCCACC ACCTGCCATG GAGCCGAGAC CTGGTGAACC CCAAAAACAA GGCTCATGCT 
CGGTACCTGA AAGAGCTGGG GGAGCAGTTT GTGGCCAGGG CTAACCACCA GGTCCTTGAG 
CACCTCCGGG AGCAGGAGGC AGGCAGGCAG GAGTTGGCGT GGCTCTACCA GGAGATCCGC 
CACCACCTGG GGCTGAGCAC CGAGGCCACC TGGACCTTTT GTGGCCGCCA GGAGCTCCTG 
GCCCAGCTGG GGCAGCGGCT CCGGCAGAGC GATGGCCATC CGCACTCACC CCTGGTGCTT 
TTTGGACCTC CAGGCATCGG AAAGACAGCG CTGATGTGCA AGTTGGCAGA ACAAATGCCG 
GGACTGCTTG GCCGCAAGAC AGTGACCGTC CTGCGGCTTC TGGGGATGTC ACAGATGAGC 
TCCGACGCCC GAGGCCTGCT CCAGAGCATC TGCTTCCAGG TGTGCCTGGC CTACGGGCTG 
CCCCTGCCCC CTGCCCAGGT CCTGGAGGCC CATGCCAGGG TGGTCCAGTT TTTCCACACC 
CTCCTCCACA CTGTCTCCTG CCGAAACTTC GAGTCACTTG TGATCCTGTT GGACTCCATG 
GATGATGTGG ATTCCATCTG CCGCGCCCGG AGGGTCCCCT GGCTGCCTCC CCGGTGCCCC 
CCGAGGATCC ACCTTATCCT CTCAGCCTGC TCGGGGAAGC CGGGGATTCT AGACATCATG 
AGACAGACGC TGACGGACCC AGAGGCCTAC TGGGAGGTGA AACCCCTCTC CGGAAACCAA 
GGGCAGGAGA TGATCCAGCT CCTGCTGGCC GCCTCGCGGA GGACGCTGAG CCCAGGGCAG 
AGGGAGCTGC TCTGGGCCAG CCTCCCAGAG TGTGGGCACC CAGGACGGCT GAGGCTGGCC 
TTTGAGGAGG CCCGGAAGTG GGCCTCCTTC ACCATACCTG CCCCTCTGGC CTCCACTGCT 
AAGGAAGCGA TCCATCAGCT GTGTGCCCGC CTGGAGCAGA CACACGGGCA GCTCCTGGTG 
GCCCACGTGC TGAGCTACAT CGTGTCCTCC CGGCATGGGC TCTCAGAGGC AGAGCTGAAG 
GACGTCCTGT CCTTGGACGA CGAGGTCCTG GAGGCGGTGT ACCAGGACTG GACCCCGCCC 
AGCAAGGAGC TGTTGCGCTT CCCCCCGTTG CTGTGGGTGA GGCTCCGTCG GGATCTCGGC 
CACTGCTTGG CCCGGCGGCC GGTGGACGGC TTCACGCTCC TGGCCATCGC CCACAGGCAG 
CTGACTGAGG TGATCCAAGA GCGCTACCTG TCTGGGCCCG AGAGAGCCAA GAGGCACGGA 
GTCTTGGCTG ACTTCTTCTC AGGGGCCTGG AGCCAGGGCA CAAAGAAACT CATCACCCTG 
CCACTCGTGG GGAAGCCCCT GAACCTGGAC CGCAAGGTGG CGCCTCAGCC CCTGTGGTTC 
TCAGACACAG TTGCAAATCT GCGGAAGCTG AAGGAGTTGC CCCATCACCT GGTCCACTCA 
GGCCGCTTCG AAGAGCTGAA GCAGGAGGTT CTGGGCAGCA TGAACTGGAT TTCCTGCCGG 
GGCGTCTCTG GGGGCATCGA GGGGCTGTTG GAGGACTTTG ACCTGTATGG CCCTCACCTG 
GATTGTCCCG AGGTCGGCCT GGTCCGGGAC GCCCTCCAGC TGTGCCGCCC GGCCCTGGAG 
CTCCGAGGCA TGGAGAGGAG CATCCTCTAC ACTGAACTCC TGGCCAGACT CCATTTCTTC 
GCCACCTCAC ATCCAGCGCT GGTGGGACAG CTGTGCCAAC AGGCCCAGAG CTGGTTCCGG 
CTGTGCCCCC ACCCTGTGCT GGTACCTCTC GGGGGGTTCC TCCAGCCCCC CGGGGGACCC 
CTCCGGGCAA CCTTCACTGG CTGTCACAAG GGCATCACTG CCATGGGCTG GAGCCTGGAA 
GAGAAGCTGC TGGTAGTTGG CACCCAGGAT GGCCTCATGG CTGTGTGGGA CATGGAAGAG 
CAGCAGGTGA TCCACATCCT AACTGGACAC ACAGGAGAGG TGAGGTCTGT GAAAGTGTTT 
GGCAAGGGGA CACTCGTCCT CTCTGCCTCA AAGGACCACA CACTGCGTTT GTGGAACTTA 
CTCTCTGGCC AGCAGAAATT CACCATTTGG GATGGAGGCT CAAAAGATCC CACTGACCCT 
GAGATCTGGA GTCTTCATGT GGATGAAGCA AAGAAGGTTG TGTATTTGGC ATCTGGCTCA 
AAGATCAGTG CTTGGAATCT GGAAACTGCA GAGCTGGTTT TCCATATCCT GGGAGATGCC 
TCCGACTGCT GGATGTGCAC AGCGGTGCTG GCTTCCCGGG CCACGCTGCT GACCGTGTCT 
GAGGGCGGTG TGGTCAGTCT GTGGAGCTCA GGCACAGGGA AACTGCAGGG GAGGCACCAT 
TTATCCAGCA TCAAAGAAGA AACACCTACC TGTGGGGTCT CGGTCCAGAA GCAAGGAAAG 
ATGGTGACTG GGTTCAGCAA AGGCTCCATC TCTTTGGTTT CCCCCGGAGG AGATAGCCTG 
CTGGAGAAGC TTCCAGAAGC CGTGAGGTTC CTGGTAGTCT CTGAAGATGA GTCCCTTCTC 
GCTGCAGGGT TTGGCAGATC CGTGCGGATA TTCTTGGCAG ACTCGCAGGG GTTTCATCGA 
TTTATGGCCA CCGACCTTGA ACATGAGGAC ACCGTGGAGA TAGCTGGTTT TGGTCCTGAG 
AACAATCTGA TTGTCACTGC GTCCCGCGAT GCACTCATCC AGGTGTGGAG CCTGTCGGAG 
CAGGGGACCC TGCTGGACAT CCTGGAAGGT GTCGGAGCCC CCGTGAGCCT GCTGGCCTGG 
GCTGGGGCCC TGGTGGCATC TGCTTCTCAG CAGTCCTCAT CTTTGAAAGT CTGGGATCTC 
ACTTACATTC AGAAGCCGAG GGCCTGCACC CCATTTCTGG ATCGTACTGG CCTCACTGCT 
GTGTCACACA ATGGAAGCTA CGTTTACTTC CCCAAAATCG GAGACAAGAA CAAAGTTACC 
ATTTGGGACT CTGCAGAAGG GGTTGTCCTG GTGTTTCCCC TGAATTCCAG ACAGGATGTG 
ATGTGCATCC CCCCTCCCGA GGCCCGGAAA GCCATCAACT GCATGGCCCT CAGTAAAGGC 
GAGGAGCGCC TGGCCATCGC CTATGACAGC ATTGTCCTTG TGCTGGACAT CAGTCCCGGG 
GATCCCTGTC CGGTTATTGA TGGGCCAACA TACACCTTCT ATACCCAGCT GCCTGAGGCC 
ATCTCCAGTG TGGCCGTTCT GGCTGACTAC CGCGTGATCT ACGGCATGAC CAACGGTGAC 
CTCTTTCTTT ACGAGTGTGC GAATTCCAAA GTGTTCCCTC TGGAGGCCCA CGGGAGCCAG 
GTCACCTGCA TGCAGGTCAG CCACAAGGAG CAGCTGGCTG TCAGCGGGTC CGAGGAAGCC 
CTGCTGTGTC TCTGGGACCT GCAGGCCTGC AAGTGGAGGT TCCAGATGAG CTACACGAAT 
TCTTACTGTA GAGGAGTCCA GTGTGTCTGC TTCTCCAAGG ATGACAAGTA TGTGTATGCG 
GGCTTGAAGG ATCGCTCCAT CATTGTCTGG AGTGTGCTGG ATGGCACCCT GCTGGCTGTC 
CAGTTTGTCC ATGCTGTGGT GAACAGGATC ATCCCAACTT CGAATGGCTT CATTGCCCCC 
ACTAGACATG GCTACCTGAT CCGCGAGCGT TTCCAGTGCC CTTCATCGAG AGTCTCACAG 
CAAGATCCTC TCAAGAACTT CAAGAAGGCA GTGTGGAAGG TCAAATCCAG GCAGAGAGAA 
GAGCTGGCAG CAGCTGCAGG AGCACTCCAG GACTCGAGAT CGGCAAGTGC CCAGGGAAAT 
GAACGTAAAT CGAACAAACG CTCACAAGTC TGCCTGCTAG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	NWD1
Entrez Gene ID	476678
Full Name	NACHT and WD repeat domain containing 1
General protein information	Preferred Names NACHT and WD repeat domain containing 1 Names NACHT and WD repeat domain-containing protein 1 NACHT domain- and WD repeat-containing protein 1
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

	LOC719640	XP_001114186.1
Canis lupus familiaris (dog)	NWD1	XP_533883.4
Xenopus tropicalis (tropical clawed frog)	nwd1	XP_002934424.2
Homo sapiens (human)	NWD1	NP_001007526.3
Pan troglodytes (chimpanzee)	NWD1	XP_003316227.2
Mus musculus (house mouse)	Nwd1	NP_795914.3
Bos taurus (cattle)	NWD1	NP_001095680.1
Rattus norvegicus (Norway rat)	LOC100364350	XP_006252881.1
Danio rerio (zebrafish)	LOC100330908	XP_002663751.3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

NWD1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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