CRIM1 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	CRIM1
Entrez Gene ID	475723
Full Name	cysteine rich transmembrane BMP regulator 1
General protein information	Preferred Names cysteine rich transmembrane BMP regulator 1 Names cysteine-rich motor neuron 1 protein
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014120280.1	XP_013975755.1	cysteine-rich motor neuron 1 protein isoform X4
XM_005630329.2	XP_005630386.1	cysteine-rich motor neuron 1 protein isoform X2
XM_532931.5	XP_532931.2	cysteine-rich motor neuron 1 protein isoform X1
XM_005630330.3	XP_005630387.1	cysteine-rich motor neuron 1 protein isoform X2
XM_005630330.2	XP_005630387.1	cysteine-rich motor neuron 1 protein isoform X3
XM_532931.4	XP_532931.2	cysteine-rich motor neuron 1 protein isoform X1
XM_014120281.1	XP_013975756.1	cysteine-rich motor neuron 1 protein isoform X5

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CRIM1	NP_001192227.1
Mus musculus (house mouse)	Crim1	NP_056615.1
Rattus norvegicus (Norway rat)	Crim1	NP_001162574.1
Homo sapiens (human)	CRIM1	NP_057525.1
Danio rerio (zebrafish)	crim1	NP_997986.1
Caenorhabditis elegans (roundworm)	crm-1	NP_741616.2
Xenopus tropicalis (tropical clawed frog)	LOC100488895	XP_002936419.1
Pan troglodytes (chimpanzee)	CRIM1	XP_001153694.2
Canis lupus familiaris (dog)	CRIM1	XP_532931.2
Gallus gallus (chicken)	CRIM1	NP_989756.1

Gene Ontology

The following CRIM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CRIM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe18880	XM_005630330.3 Latest version!	Canis lupus familiaris cysteine rich transmembrane BMP regulator 1 (CRIM1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe40692	XM_014120280.1 Latest version!	Canis lupus familiaris cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OCe40693	XM_014120281.1 Latest version!	Canis lupus familiaris cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OCe18878	XM_532931.5 Latest version!	Canis lupus familiaris cysteine rich transmembrane BMP regulator 1 (CRIM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OCe18879	XM_005630329.2 Latest version!	Canis lupus familiaris cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OCe18880	XM_005630330.2	Canis lupus familiaris cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe18878	XM_532931.4	Canis lupus familiaris cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CRIM1 ( XM_005630330.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005630387.1 Canis lupus familiaris cysteine rich transmembrane BMP regulator 1 (CRIM1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe18880
Clone ID Related Accession (Same CDS sequence)	XM_005630330.3 , XM_005630330.2
Accession Version	XM_005630330.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2904bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	cysteine-rich motor neuron 1 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005630330.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTTGG TGGCGGGGGG CAGGGGGCTG GCCGGCTGCG GGCACCTCCC GGTCTCGCTG 
CTGGGGCTGC TGCTGCTGCT GGCGCGCTCC GGCACCCGGG CGCTGGTCTG CCTGCCCTGC 
GACGAGTCCA AGTGCGAGGA GCCCAGGAGC TGCCCCGGGA GCATCGTGCA GGGCGTCTGC 
GGCTGCTGCT ACATGTGCGC CCGCCAGAGG AACGAGAGCT GCGGCGGCGC CTACGGGCTC 
CACGGAGCCT GCGACCGGGG GCTGCGCTGT GTCATCCGCC CCCCGCTCAA TGGCGACTCC 
ATCACCGAGT ACGAAGTGGG CGTCTGCGAA GATGAGAACT GGGATGATGA CCAGCTGCTT 
GGTTTTGAAC CCTGCAATGA AAATCTTGTG TCTGGCTGCA ATATAATCAA TGGGAAATGC 
GAATGTGACA CCATTCGCAC CTGCAACAAT CCCTTTGAGT TTCCAAGGAA GGATATGTGC 
CTTTCAGCTT TGAAGAGGAT TGAAGAAGAG AAGCCAGATT GCTCCAAGGC CCGCTGTGAA 
GTCCAGTTCT CTCCACGTTG TCCTGAAGAT TCCGTTCTGA TCGAGGGCTA TGCTCCCCCC 
GGGGAGTGCT GCCCCTTACC CAGCCGCTGC GTGTGCGACC CTGCGGGCTG TCTGCGCAAA 
GTCTGCCAGC CGGGATACCT GAACATACTA GTGTCAAAGG CCTCAGGGAA GCCGGGAGAG 
TGTTGTGACC TCTATGAGTG CAAACCAGTT TTCAGCGTGG ACTGCAGCAC CGTGGAGTGC 
CCCTCCGTTC AGCAGGCCGT GTGCCCTCTG GACAGCTACG AGACTCAAGT CAGACTCACG 
GCGGACGGTT GCTGCACCCT GCCAACAAGA TGCGAGTGTC TCTCTGGCTT ATGTGGTTTC 
CCCGTGTGTG AGGTGGGATC CACTCCTCGC ATAGTCTCTC GTGGAGATGG GACACCTGGA 
AAGTGCTGTG ATGTCTTTGA ATGTGTTAAT GAAACAAAGC CAGCCTGTGT ATTCAACAAC 
GTGGAATATA ACGATGGGGA TATGTTTCGA ATGGACAACT GTCGATTCTG TCGATGCCAG 
GGGGGTGTTT CCATCTGCTT CACTGCCCAG TGTGGTGAGC TGAACTGCGA GAGGTATTAT 
GTGCCCGAAG GAGAGTGCTG CCCAGTGTGT GAAGATCCGG TGTATCCTTT TAATAATCCT 
GCTGGCTGCT ATGCCAATGG TCAGATCCGC GCGCACGGGG ACCGGTGGCG AGAAGACGAC 
TGCACTTTCT GCCAGTGTAT CAATGGAGAA CCCCACTGTG TGGCCACGGC CTGTGGGCAG 
AGCTGCATGA ACCCCGTCAA AGTGCCTGGC GAGTGTTGCC CTGTGTGTGA AGAGCCAACG 
TACATCACAA TTGATCCACC TGCATGTGGG GAGTTATCAA ACTGCACGCT GACAGAGAAG 
GACTGCGTTT ATGGTTTCAA ACTTGATCAC AATGGTTGTC GAACCTGTCA GTGCAAAAAC 
AGGGAGGAGC TGTGCTCGGG CCTCAAGCGA GGCTGTACTT TGGACTGCCC CTTCGGCTTC 
CTGACTGATG CCCACAACTG TGAGATCTGT CAGTGCCGCC CACGGCCCAA GAAATGCAGA 
CCTGTAATCT GTGACAAGTA TTGTCCATTT GGATATCTGA AGAATAAGCA TGGCTGTAAC 
ATCTGTCGCT GCAAGAAATG CCCAGAGCTC CTGTGCAGTA AAATTTGCCC CTTGGGTTTC 
CAGCAGGACA GTCATGGCTG TCTTATCTGC AAGTGCAGAG ATGACTTTGT GGTGCAAAAG 
CCAGAGCTCA GCACCCCCTC CATTTGCCAT GCCCCTGGAG GAGAGTACTT CGTGGAAGGA 
GAGACATGGA ACATCGACTC GTGTACTCAG TGCACCTGCC ACAGCGGGCG GGTGCTGTGT 
GAGACGGAGG TGTGCCCACC GCTGCTCTGC GAGAACCCCT CGCGCACCCA GGACTCCTGC 
TGCCCGCAGT GCACAGATGA ACCTCTTCAG CCTTCCTCAT CCCATAATGA CAGTGTGCCT 
AGTTACTGCA AAAATGACGA AGGGGATATA TTCCTGGCAG CCGAGTCCTG GAAGCCTGAC 
GTCTGTACCA GCTGTGTATG CATGGATAGC GTAATTAGCT GTTACTCTGA ATCCTGCCCT 
TCAGTATCCT GTGAAAGACC TGTTTTGAGA AAAGGCCAGT GTTGTCCCTA TTGCATAGAA 
GACACGATTC CGAAGAAGGT GGTGTGCCAC TTCAGTGGGA AGGCCTACGC CGACGAGGAG 
CGCTGGGACA TTGACAGCTG CACACACTGC TACTGCCTGC AGGGCCAGAC CCTCTGCTCG 
ACCGTCAGCT GCCCCCCTCT GCCCTGTGTC GAGCCCATCA ACGTGGAAGG CAGTTGCTGC 
CCAATGTGTC CAGAAATGTA TGTCCCAGAA CCAACCAATA TACCCATTGA GAAAACAAAT 
CACCGTGGCG ACATCGACCT GGAGGTTCCC TTGTGGCCTA CACCAAGTGA AAATGACATC 
ATCCATCTTC CCAGAGACAT GGGTCACCTC CAGGTAGATT ACAGAGATAA CAACAGGCTG 
CATTCAGGCG AAGATTCTTC ACTGGACTCC ATTGCCTCGG TTGTGGTTCC CATCATCATC 
GGCCTCTCGA TTATAATAGC ATTCCTGTTC ATCAATCAGA AGAAACAGTG GATACCACTG 
CTTTGCTGGT ATCGAACACC AACTAAGCCT TCTTCCTTAA ATAATCAGCT GGTGTCTGTG 
GACTGCAAGA AAGGAAGCAG AGTCCCAGTG GACAGTTCAC AGAGAATGCT AAGAATTGCT 
GAACCAGATG CAAGATTCAG TGGCTTCTAC AGCATGCAAA AACAGAACCA CCTACAGGCA 
GATAATTTCT ACCAGACAGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005630387.1
CDS	10..2913
Translation	MYLVAGGRGLAGCGHLPVSLLGLLLLLARSGTRALVCLPCDESKCEEPRSCPGSIVQGVCGCCYMCARQRNESCGGAYGLHGACDRGLRCVIRPPLNGDSITEYEVGVCEDENWDDDQLLGFEPCNENLVSGCNIINGKCECDTIRTCNNPFEFPRKDMCLSALKRIEEEKPDCSKARCEVQFSPRCPEDSVLIEGYAPPGECCPLPSRCVCDPAGCLRKVCQPGYLNILVSKASGKPGECCDLYECKPVFSVDCSTVECPSVQQAVCPLDSYETQVRLTADGCCTLPTRCECLSGLCGFPVCEVGSTPRIVSRGDGTPGKCCDVFECVNETKPACVFNNVEYNDGDMFRMDNCRFCRCQGGVSICFTAQCGELNCERYYVPEGECCPVCEDPVYPFNNPAGCYANGQIRAHGDRWREDDCTFCQCINGEPHCVATACGQSCMNPVKVPGECCPVCEEPTYITIDPPACGELSNCTLTEKDCVYGFKLDHNGCRTCQCKNREELCSGLKRGCTLDCPFGFLTDAHNCEICQCRPRPKKCRPVICDKYCPFGYLKNKHGCNICRCKKCPELLCSKICPLGFQQDSHGCLICKCRDDFVVQKPELSTPSICHAPGGEYFVEGETWNIDSCTQCTCHSGRVLCETEVCPPLLCENPSRTQDSCCPQCTDEPLQPSSSHNDSVPSYCKNDEGDIFLAAESWKPDVCTSCVCMDSVISCYSESCPSVSCERPVLRKGQCCPYCIEDTIPKKVVCHFSGKAYADEERWDIDSCTHCYCLQGQTLCSTVSCPPLPCVEPINVEGSCCPMCPEMYVPEPTNIPIEKTNHRGDIDLEVPLWPTPSENDIIHLPRDMGHLQVDYRDNNRLHSGEDSSLDSIASVVVPIIIGLSIIIAFLFINQKKQWIPLLCWYRTPTKPSSLNNQLVSVDCKKGSRVPVDSSQRMLRIAEPDARFSGFYSMQKQNHLQADNFYQTV

Target ORF information:

RefSeq Version	XM_005630330.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris cysteine rich transmembrane BMP regulator 1 (CRIM1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005630330.3

ORF Insert Sequence:

ATGTACTTGG TGGCGGGGGG CAGGGGGCTG GCCGGCTGCG GGCACCTCCC GGTCTCGCTG 
CTGGGGCTGC TGCTGCTGCT GGCGCGCTCC GGCACCCGGG CGCTGGTCTG CCTGCCCTGC 
GACGAGTCCA AGTGCGAGGA GCCCAGGAGC TGCCCCGGGA GCATCGTGCA GGGCGTCTGC 
GGCTGCTGCT ACATGTGCGC CCGCCAGAGG AACGAGAGCT GCGGCGGCGC CTACGGGCTC 
CACGGAGCCT GCGACCGGGG GCTGCGCTGT GTCATCCGCC CCCCGCTCAA TGGCGACTCC 
ATCACCGAGT ACGAAGTGGG CGTCTGCGAA GATGAGAACT GGGATGATGA CCAGCTGCTT 
GGTTTTGAAC CCTGCAATGA AAATCTTGTG TCTGGCTGCA ATATAATCAA TGGGAAATGC 
GAATGTGACA CCATTCGCAC CTGCAACAAT CCCTTTGAGT TTCCAAGGAA GGATATGTGC 
CTTTCAGCTT TGAAGAGGAT TGAAGAAGAG AAGCCAGATT GCTCCAAGGC CCGCTGTGAA 
GTCCAGTTCT CTCCACGTTG TCCTGAAGAT TCCGTTCTGA TCGAGGGCTA TGCTCCCCCC 
GGGGAGTGCT GCCCCTTACC CAGCCGCTGC GTGTGCGACC CTGCGGGCTG TCTGCGCAAA 
GTCTGCCAGC CGGGATACCT GAACATACTA GTGTCAAAGG CCTCAGGGAA GCCGGGAGAG 
TGTTGTGACC TCTATGAGTG CAAACCAGTT TTCAGCGTGG ACTGCAGCAC CGTGGAGTGC 
CCCTCCGTTC AGCAGGCCGT GTGCCCTCTG GACAGCTACG AGACTCAAGT CAGACTCACG 
GCGGACGGTT GCTGCACCCT GCCAACAAGA TGCGAGTGTC TCTCTGGCTT ATGTGGTTTC 
CCCGTGTGTG AGGTGGGATC CACTCCTCGC ATAGTCTCTC GTGGAGATGG GACACCTGGA 
AAGTGCTGTG ATGTCTTTGA ATGTGTTAAT GAAACAAAGC CAGCCTGTGT ATTCAACAAC 
GTGGAATATA ACGATGGGGA TATGTTTCGA ATGGACAACT GTCGATTCTG TCGATGCCAG 
GGGGGTGTTT CCATCTGCTT CACTGCCCAG TGTGGTGAGC TGAACTGCGA GAGGTATTAT 
GTGCCCGAAG GAGAGTGCTG CCCAGTGTGT GAAGATCCGG TGTATCCTTT TAATAATCCT 
GCTGGCTGCT ATGCCAATGG TCAGATCCGC GCGCACGGGG ACCGGTGGCG AGAAGACGAC 
TGCACTTTCT GCCAGTGTAT CAATGGAGAA CCCCACTGTG TGGCCACGGC CTGTGGGCAG 
AGCTGCATGA ACCCCGTCAA AGTGCCTGGC GAGTGTTGCC CTGTGTGTGA AGAGCCAACG 
TACATCACAA TTGATCCACC TGCATGTGGG GAGTTATCAA ACTGCACGCT GACAGAGAAG 
GACTGCGTTT ATGGTTTCAA ACTTGATCAC AATGGTTGTC GAACCTGTCA GTGCAAAAAC 
AGGGAGGAGC TGTGCTCGGG CCTCAAGCGA GGCTGTACTT TGGACTGCCC CTTCGGCTTC 
CTGACTGATG CCCACAACTG TGAGATCTGT CAGTGCCGCC CACGGCCCAA GAAATGCAGA 
CCTGTAATCT GTGACAAGTA TTGTCCATTT GGATATCTGA AGAATAAGCA TGGCTGTAAC 
ATCTGTCGCT GCAAGAAATG CCCAGAGCTC CTGTGCAGTA AAATTTGCCC CTTGGGTTTC 
CAGCAGGACA GTCATGGCTG TCTTATCTGC AAGTGCAGAG ATGACTTTGT GGTGCAAAAG 
CCAGAGCTCA GCACCCCCTC CATTTGCCAT GCCCCTGGAG GAGAGTACTT CGTGGAAGGA 
GAGACATGGA ACATCGACTC GTGTACTCAG TGCACCTGCC ACAGCGGGCG GGTGCTGTGT 
GAGACGGAGG TGTGCCCACC GCTGCTCTGC GAGAACCCCT CGCGCACCCA GGACTCCTGC 
TGCCCGCAGT GCACAGATGA ACCTCTTCAG CCTTCCTCAT CCCATAATGA CAGTGTGCCT 
AGTTACTGCA AAAATGACGA AGGGGATATA TTCCTGGCAG CCGAGTCCTG GAAGCCTGAC 
GTCTGTACCA GCTGTGTATG CATGGATAGC GTAATTAGCT GTTACTCTGA ATCCTGCCCT 
TCAGTATCCT GTGAAAGACC TGTTTTGAGA AAAGGCCAGT GTTGTCCCTA TTGCATAGAA 
GACACGATTC CGAAGAAGGT GGTGTGCCAC TTCAGTGGGA AGGCCTACGC CGACGAGGAG 
CGCTGGGACA TTGACAGCTG CACACACTGC TACTGCCTGC AGGGCCAGAC CCTCTGCTCG 
ACCGTCAGCT GCCCCCCTCT GCCCTGTGTC GAGCCCATCA ACGTGGAAGG CAGTTGCTGC 
CCAATGTGTC CAGAAATGTA TGTCCCAGAA CCAACCAATA TACCCATTGA GAAAACAAAT 
CACCGTGGCG ACATCGACCT GGAGGTTCCC TTGTGGCCTA CACCAAGTGA AAATGACATC 
ATCCATCTTC CCAGAGACAT GGGTCACCTC CAGGTAGATT ACAGAGATAA CAACAGGCTG 
CATTCAGGCG AAGATTCTTC ACTGGACTCC ATTGCCTCGG TTGTGGTTCC CATCATCATC 
GGCCTCTCGA TTATAATAGC ATTCCTGTTC ATCAATCAGA AGAAACAGTG GATACCACTG 
CTTTGCTGGT ATCGAACACC AACTAAGCCT TCTTCCTTAA ATAATCAGCT GGTGTCTGTG 
GACTGCAAGA AAGGAAGCAG AGTCCCAGTG GACAGTTCAC AGAGAATGCT AAGAATTGCT 
GAACCAGATG CAAGATTCAG TGGCTTCTAC AGCATGCAAA AACAGAACCA CCTACAGGCA 
GATAATTTCT ACCAGACAGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRIM1 ( XM_005630330.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005630387.1 Canis lupus familiaris cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe18880
Clone ID Related Accession (Same CDS sequence)	XM_005630330.3 , XM_005630330.2
Accession Version	XM_005630330.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2904bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	cysteine-rich motor neuron 1 protein isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005630330.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTTGG TGGCGGGGGG CAGGGGGCTG GCCGGCTGCG GGCACCTCCC GGTCTCGCTG 
CTGGGGCTGC TGCTGCTGCT GGCGCGCTCC GGCACCCGGG CGCTGGTCTG CCTGCCCTGC 
GACGAGTCCA AGTGCGAGGA GCCCAGGAGC TGCCCCGGGA GCATCGTGCA GGGCGTCTGC 
GGCTGCTGCT ACATGTGCGC CCGCCAGAGG AACGAGAGCT GCGGCGGCGC CTACGGGCTC 
CACGGAGCCT GCGACCGGGG GCTGCGCTGT GTCATCCGCC CCCCGCTCAA TGGCGACTCC 
ATCACCGAGT ACGAAGTGGG CGTCTGCGAA GATGAGAACT GGGATGATGA CCAGCTGCTT 
GGTTTTGAAC CCTGCAATGA AAATCTTGTG TCTGGCTGCA ATATAATCAA TGGGAAATGC 
GAATGTGACA CCATTCGCAC CTGCAACAAT CCCTTTGAGT TTCCAAGGAA GGATATGTGC 
CTTTCAGCTT TGAAGAGGAT TGAAGAAGAG AAGCCAGATT GCTCCAAGGC CCGCTGTGAA 
GTCCAGTTCT CTCCACGTTG TCCTGAAGAT TCCGTTCTGA TCGAGGGCTA TGCTCCCCCC 
GGGGAGTGCT GCCCCTTACC CAGCCGCTGC GTGTGCGACC CTGCGGGCTG TCTGCGCAAA 
GTCTGCCAGC CGGGATACCT GAACATACTA GTGTCAAAGG CCTCAGGGAA GCCGGGAGAG 
TGTTGTGACC TCTATGAGTG CAAACCAGTT TTCAGCGTGG ACTGCAGCAC CGTGGAGTGC 
CCCTCCGTTC AGCAGGCCGT GTGCCCTCTG GACAGCTACG AGACTCAAGT CAGACTCACG 
GCGGACGGTT GCTGCACCCT GCCAACAAGA TGCGAGTGTC TCTCTGGCTT ATGTGGTTTC 
CCCGTGTGTG AGGTGGGATC CACTCCTCGC ATAGTCTCTC GTGGAGATGG GACACCTGGA 
AAGTGCTGTG ATGTCTTTGA ATGTGTTAAT GAAACAAAGC CAGCCTGTGT ATTCAACAAC 
GTGGAATATA ACGATGGGGA TATGTTTCGA ATGGACAACT GTCGATTCTG TCGATGCCAG 
GGGGGTGTTT CCATCTGCTT CACTGCCCAG TGTGGTGAGC TGAACTGCGA GAGGTATTAT 
GTGCCCGAAG GAGAGTGCTG CCCAGTGTGT GAAGATCCGG TGTATCCTTT TAATAATCCT 
GCTGGCTGCT ATGCCAATGG TCAGATCCGC GCGCACGGGG ACCGGTGGCG AGAAGACGAC 
TGCACTTTCT GCCAGTGTAT CAATGGAGAA CCCCACTGTG TGGCCACGGC CTGTGGGCAG 
AGCTGCATGA ACCCCGTCAA AGTGCCTGGC GAGTGTTGCC CTGTGTGTGA AGAGCCAACG 
TACATCACAA TTGATCCACC TGCATGTGGG GAGTTATCAA ACTGCACGCT GACAGAGAAG 
GACTGCGTTT ATGGTTTCAA ACTTGATCAC AATGGTTGTC GAACCTGTCA GTGCAAAAAC 
AGGGAGGAGC TGTGCTCGGG CCTCAAGCGA GGCTGTACTT TGGACTGCCC CTTCGGCTTC 
CTGACTGATG CCCACAACTG TGAGATCTGT CAGTGCCGCC CACGGCCCAA GAAATGCAGA 
CCTGTAATCT GTGACAAGTA TTGTCCATTT GGATATCTGA AGAATAAGCA TGGCTGTAAC 
ATCTGTCGCT GCAAGAAATG CCCAGAGCTC CTGTGCAGTA AAATTTGCCC CTTGGGTTTC 
CAGCAGGACA GTCATGGCTG TCTTATCTGC AAGTGCAGAG ATGACTTTGT GGTGCAAAAG 
CCAGAGCTCA GCACCCCCTC CATTTGCCAT GCCCCTGGAG GAGAGTACTT CGTGGAAGGA 
GAGACATGGA ACATCGACTC GTGTACTCAG TGCACCTGCC ACAGCGGGCG GGTGCTGTGT 
GAGACGGAGG TGTGCCCACC GCTGCTCTGC GAGAACCCCT CGCGCACCCA GGACTCCTGC 
TGCCCGCAGT GCACAGATGA ACCTCTTCAG CCTTCCTCAT CCCATAATGA CAGTGTGCCT 
AGTTACTGCA AAAATGACGA AGGGGATATA TTCCTGGCAG CCGAGTCCTG GAAGCCTGAC 
GTCTGTACCA GCTGTGTATG CATGGATAGC GTAATTAGCT GTTACTCTGA ATCCTGCCCT 
TCAGTATCCT GTGAAAGACC TGTTTTGAGA AAAGGCCAGT GTTGTCCCTA TTGCATAGAA 
GACACGATTC CGAAGAAGGT GGTGTGCCAC TTCAGTGGGA AGGCCTACGC CGACGAGGAG 
CGCTGGGACA TTGACAGCTG CACACACTGC TACTGCCTGC AGGGCCAGAC CCTCTGCTCG 
ACCGTCAGCT GCCCCCCTCT GCCCTGTGTC GAGCCCATCA ACGTGGAAGG CAGTTGCTGC 
CCAATGTGTC CAGAAATGTA TGTCCCAGAA CCAACCAATA TACCCATTGA GAAAACAAAT 
CACCGTGGCG ACATCGACCT GGAGGTTCCC TTGTGGCCTA CACCAAGTGA AAATGACATC 
ATCCATCTTC CCAGAGACAT GGGTCACCTC CAGGTAGATT ACAGAGATAA CAACAGGCTG 
CATTCAGGCG AAGATTCTTC ACTGGACTCC ATTGCCTCGG TTGTGGTTCC CATCATCATC 
GGCCTCTCGA TTATAATAGC ATTCCTGTTC ATCAATCAGA AGAAACAGTG GATACCACTG 
CTTTGCTGGT ATCGAACACC AACTAAGCCT TCTTCCTTAA ATAATCAGCT GGTGTCTGTG 
GACTGCAAGA AAGGAAGCAG AGTCCCAGTG GACAGTTCAC AGAGAATGCT AAGAATTGCT 
GAACCAGATG CAAGATTCAG TGGCTTCTAC AGCATGCAAA AACAGAACCA CCTACAGGCA 
GATAATTTCT ACCAGACAGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005630387.1
CDS	10..2913
Translation	MYLVAGGRGLAGCGHLPVSLLGLLLLLARSGTRALVCLPCDESKCEEPRSCPGSIVQGVCGCCYMCARQRNESCGGAYGLHGACDRGLRCVIRPPLNGDSITEYEVGVCEDENWDDDQLLGFEPCNENLVSGCNIINGKCECDTIRTCNNPFEFPRKDMCLSALKRIEEEKPDCSKARCEVQFSPRCPEDSVLIEGYAPPGECCPLPSRCVCDPAGCLRKVCQPGYLNILVSKASGKPGECCDLYECKPVFSVDCSTVECPSVQQAVCPLDSYETQVRLTADGCCTLPTRCECLSGLCGFPVCEVGSTPRIVSRGDGTPGKCCDVFECVNETKPACVFNNVEYNDGDMFRMDNCRFCRCQGGVSICFTAQCGELNCERYYVPEGECCPVCEDPVYPFNNPAGCYANGQIRAHGDRWREDDCTFCQCINGEPHCVATACGQSCMNPVKVPGECCPVCEEPTYITIDPPACGELSNCTLTEKDCVYGFKLDHNGCRTCQCKNREELCSGLKRGCTLDCPFGFLTDAHNCEICQCRPRPKKCRPVICDKYCPFGYLKNKHGCNICRCKKCPELLCSKICPLGFQQDSHGCLICKCRDDFVVQKPELSTPSICHAPGGEYFVEGETWNIDSCTQCTCHSGRVLCETEVCPPLLCENPSRTQDSCCPQCTDEPLQPSSSHNDSVPSYCKNDEGDIFLAAESWKPDVCTSCVCMDSVISCYSESCPSVSCERPVLRKGQCCPYCIEDTIPKKVVCHFSGKAYADEERWDIDSCTHCYCLQGQTLCSTVSCPPLPCVEPINVEGSCCPMCPEMYVPEPTNIPIEKTNHRGDIDLEVPLWPTPSENDIIHLPRDMGHLQVDYRDNNRLHSGEDSSLDSIASVVVPIIIGLSIIIAFLFINQKKQWIPLLCWYRTPTKPSSLNNQLVSVDCKKGSRVPVDSSQRMLRIAEPDARFSGFYSMQKQNHLQADNFYQTV

Target ORF information:

RefSeq Version	XM_005630330.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005630330.2

ORF Insert Sequence:

ATGTACTTGG TGGCGGGGGG CAGGGGGCTG GCCGGCTGCG GGCACCTCCC GGTCTCGCTG 
CTGGGGCTGC TGCTGCTGCT GGCGCGCTCC GGCACCCGGG CGCTGGTCTG CCTGCCCTGC 
GACGAGTCCA AGTGCGAGGA GCCCAGGAGC TGCCCCGGGA GCATCGTGCA GGGCGTCTGC 
GGCTGCTGCT ACATGTGCGC CCGCCAGAGG AACGAGAGCT GCGGCGGCGC CTACGGGCTC 
CACGGAGCCT GCGACCGGGG GCTGCGCTGT GTCATCCGCC CCCCGCTCAA TGGCGACTCC 
ATCACCGAGT ACGAAGTGGG CGTCTGCGAA GATGAGAACT GGGATGATGA CCAGCTGCTT 
GGTTTTGAAC CCTGCAATGA AAATCTTGTG TCTGGCTGCA ATATAATCAA TGGGAAATGC 
GAATGTGACA CCATTCGCAC CTGCAACAAT CCCTTTGAGT TTCCAAGGAA GGATATGTGC 
CTTTCAGCTT TGAAGAGGAT TGAAGAAGAG AAGCCAGATT GCTCCAAGGC CCGCTGTGAA 
GTCCAGTTCT CTCCACGTTG TCCTGAAGAT TCCGTTCTGA TCGAGGGCTA TGCTCCCCCC 
GGGGAGTGCT GCCCCTTACC CAGCCGCTGC GTGTGCGACC CTGCGGGCTG TCTGCGCAAA 
GTCTGCCAGC CGGGATACCT GAACATACTA GTGTCAAAGG CCTCAGGGAA GCCGGGAGAG 
TGTTGTGACC TCTATGAGTG CAAACCAGTT TTCAGCGTGG ACTGCAGCAC CGTGGAGTGC 
CCCTCCGTTC AGCAGGCCGT GTGCCCTCTG GACAGCTACG AGACTCAAGT CAGACTCACG 
GCGGACGGTT GCTGCACCCT GCCAACAAGA TGCGAGTGTC TCTCTGGCTT ATGTGGTTTC 
CCCGTGTGTG AGGTGGGATC CACTCCTCGC ATAGTCTCTC GTGGAGATGG GACACCTGGA 
AAGTGCTGTG ATGTCTTTGA ATGTGTTAAT GAAACAAAGC CAGCCTGTGT ATTCAACAAC 
GTGGAATATA ACGATGGGGA TATGTTTCGA ATGGACAACT GTCGATTCTG TCGATGCCAG 
GGGGGTGTTT CCATCTGCTT CACTGCCCAG TGTGGTGAGC TGAACTGCGA GAGGTATTAT 
GTGCCCGAAG GAGAGTGCTG CCCAGTGTGT GAAGATCCGG TGTATCCTTT TAATAATCCT 
GCTGGCTGCT ATGCCAATGG TCAGATCCGC GCGCACGGGG ACCGGTGGCG AGAAGACGAC 
TGCACTTTCT GCCAGTGTAT CAATGGAGAA CCCCACTGTG TGGCCACGGC CTGTGGGCAG 
AGCTGCATGA ACCCCGTCAA AGTGCCTGGC GAGTGTTGCC CTGTGTGTGA AGAGCCAACG 
TACATCACAA TTGATCCACC TGCATGTGGG GAGTTATCAA ACTGCACGCT GACAGAGAAG 
GACTGCGTTT ATGGTTTCAA ACTTGATCAC AATGGTTGTC GAACCTGTCA GTGCAAAAAC 
AGGGAGGAGC TGTGCTCGGG CCTCAAGCGA GGCTGTACTT TGGACTGCCC CTTCGGCTTC 
CTGACTGATG CCCACAACTG TGAGATCTGT CAGTGCCGCC CACGGCCCAA GAAATGCAGA 
CCTGTAATCT GTGACAAGTA TTGTCCATTT GGATATCTGA AGAATAAGCA TGGCTGTAAC 
ATCTGTCGCT GCAAGAAATG CCCAGAGCTC CTGTGCAGTA AAATTTGCCC CTTGGGTTTC 
CAGCAGGACA GTCATGGCTG TCTTATCTGC AAGTGCAGAG ATGACTTTGT GGTGCAAAAG 
CCAGAGCTCA GCACCCCCTC CATTTGCCAT GCCCCTGGAG GAGAGTACTT CGTGGAAGGA 
GAGACATGGA ACATCGACTC GTGTACTCAG TGCACCTGCC ACAGCGGGCG GGTGCTGTGT 
GAGACGGAGG TGTGCCCACC GCTGCTCTGC GAGAACCCCT CGCGCACCCA GGACTCCTGC 
TGCCCGCAGT GCACAGATGA ACCTCTTCAG CCTTCCTCAT CCCATAATGA CAGTGTGCCT 
AGTTACTGCA AAAATGACGA AGGGGATATA TTCCTGGCAG CCGAGTCCTG GAAGCCTGAC 
GTCTGTACCA GCTGTGTATG CATGGATAGC GTAATTAGCT GTTACTCTGA ATCCTGCCCT 
TCAGTATCCT GTGAAAGACC TGTTTTGAGA AAAGGCCAGT GTTGTCCCTA TTGCATAGAA 
GACACGATTC CGAAGAAGGT GGTGTGCCAC TTCAGTGGGA AGGCCTACGC CGACGAGGAG 
CGCTGGGACA TTGACAGCTG CACACACTGC TACTGCCTGC AGGGCCAGAC CCTCTGCTCG 
ACCGTCAGCT GCCCCCCTCT GCCCTGTGTC GAGCCCATCA ACGTGGAAGG CAGTTGCTGC 
CCAATGTGTC CAGAAATGTA TGTCCCAGAA CCAACCAATA TACCCATTGA GAAAACAAAT 
CACCGTGGCG ACATCGACCT GGAGGTTCCC TTGTGGCCTA CACCAAGTGA AAATGACATC 
ATCCATCTTC CCAGAGACAT GGGTCACCTC CAGGTAGATT ACAGAGATAA CAACAGGCTG 
CATTCAGGCG AAGATTCTTC ACTGGACTCC ATTGCCTCGG TTGTGGTTCC CATCATCATC 
GGCCTCTCGA TTATAATAGC ATTCCTGTTC ATCAATCAGA AGAAACAGTG GATACCACTG 
CTTTGCTGGT ATCGAACACC AACTAAGCCT TCTTCCTTAA ATAATCAGCT GGTGTCTGTG 
GACTGCAAGA AAGGAAGCAG AGTCCCAGTG GACAGTTCAC AGAGAATGCT AAGAATTGCT 
GAACCAGATG CAAGATTCAG TGGCTTCTAC AGCATGCAAA AACAGAACCA CCTACAGGCA 
GATAATTTCT ACCAGACAGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRIM1 ( XM_014120280.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013975755.1 Canis lupus familiaris cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe40692
Clone ID Related Accession (Same CDS sequence)	XM_014120280.1
Accession Version	XM_014120280.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	cysteine-rich motor neuron 1 protein isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTTGG TGGCGGGGGG CAGGGGGCTG GCCGGCTGCG GGCACCTCCC GGTCTCGCTG 
CTGGGGCTGC TGCTGCTGCT GGCGCGCTCC GGCACCCGGG CGCTGGTCTG CCTGCCCTGC 
GACGAGTCCA AGTGCGAGGA GCCCAGGAGC TGCCCCGGGA GCATCGTGCA GGGCGTCTGC 
GGCTGCTGCT ACATGTGCGC CCGCCAGAGG AACGAGAGCT GCGGCGGCGC CTACGGGCTC 
CACGGAGCCT GCGACCGGGG GCTGCGCTGT GTCATCCGCC CCCCGCTCAA TGGCGACTCC 
ATCACCGAGT ACGAAGTGGG CGTCTGCGAA GATGAGAACT GGGATGATGA CCAGCTGCTT 
GGTTTTGAAC CCTGCAATGA AAATCTTGTG TCTGGCTGCA ATATAATCAA TGGGAAATGC 
GAATGTGACA CCATTCGCAC CTGCAACAAT CCCTTTGAGT TTCCAAGGAA GGATATGTGC 
CTTTCAGCTT TGAAGAGGAT TGAAGTTTTC AGCGTGGACT GCAGCACCGT GGAGTGCCCC 
TCCGTTCAGC AGGCCGTGTG CCCTCTGGAC AGCTACGAGA CTCAAGTCAG ACTCACGGCG 
GACGGTTGCT GCACCCTGCC AACAAGATGC GAGTGTCTCT CTGGCTTATG TGGTTTCCCC 
GTGTGTGAGG TGGGATCCAC TCCTCGCATA GTCTCTCGTG GAGATGGGAC ACCTGGAAAG 
TGCTGTGATG TCTTTGAATG TGTTAATGAA ACAAAGCCAG CCTGTGTATT CAACAACGTG 
GAATATAACG ATGGGGATAT GTTTCGAATG GACAACTGTC GATTCTGTCG ATGCCAGGGG 
GGTGTTTCCA TCTGCTTCAC TGCCCAGTGT GGTGAGCTGA ACTGCGAGAG GTATTATGTG 
CCCGAAGGAG AGTGCTGCCC AGTGTGTGAA GATCCGGTGT ATCCTTTTAA TAATCCTGCT 
GGCTGCTATG CCAATGGTCA GATCCGCGCG CACGGGGACC GGTGGCGAGA AGACGACTGC 
ACTTTCTGCC AGTGTATCAA TGGAGAACCC CACTGTGTGG CCACGGCCTG TGGGCAGAGC 
TGCATGAACC CCGTCAAAGT GCCTGGCGAG TGTTGCCCTG TGTGTGAAGA GCCAACGTAC 
ATCACAATTG ATCCACCTGC ATGTGGGGAG TTATCAAACT GCACGCTGAC AGAGAAGGAC 
TGCGTTTATG GTTTCAAACT TGATCACAAT GGTTGTCGAA CCTGTCAGTG CAAAAACAGG 
GAGGAGCTGT GCTCGGGCCT CAAGCGAGGC TGTACTTTGG ACTGCCCCTT CGGCTTCCTG 
ACTGATGCCC ACAACTGTGA GATCTGTCAG TGCCGCCCAC GGCCCAAGAA ATGCAGACCT 
GTAATCTGTG ACAAGTATTG TCCATTTGGA TATCTGAAGA ATAAGCATGG CTGTAACATC 
TGTCGCTGCA AGAAATGCCC AGAGCTCCTG TGCAGTAAAA TTTGCCCCTT GGGTTTCCAG 
CAGGACAGTC ATGGCTGTCT TATCTGCAAG TGCAGAGAGG TCCTTGCTTC AGCCGGGCCA 
CCTGTCCTGT CAGGCACATG TCTGTCCATG GATGGCCATC ATCATAAAAA TGAGGAAAGC 
TGGCATGATG GGTGCCGGGA ATGCTACTGT CACAATGGAC GGGAAATGTG TGCTCTGATC 
ACCTGCCCGG TGCCTGCCTG TGGCAACCCC ACCATTCATC CCGGACAGTG CTGCCCGTCA 
TGTTCAGATG ACTTTGTGGT GCAAAAGCCA GAGCTCAGCA CCCCCTCCAT TTGCCATGCC 
CCTGGAGGAG AGTACTTCGT GGAAGGAGAG ACATGGAACA TCGACTCGTG TACTCAGTGC 
ACCTGCCACA GCGGGCGGGT GCTGTGTGAG ACGGAGGTGT GCCCACCGCT GCTCTGCGAG 
AACCCCTCGC GCACCCAGGA CTCCTGCTGC CCGCAGTGCA CAGATGAACC TCTTCAGCCT 
TCCTCATCCC ATAATGACAG TGTGCCTAGT TACTGCAAAA ATGACGAAGG GGATATATTC 
CTGGCAGCCG AGTCCTGGAA GCCTGACGTC TGTACCAGCT GTGTATGCAT GGATAGCGTA 
ATTAGCTGTT ACTCTGAATC CTGCCCTTCA GTATCCTGTG AAAGACCTGT TTTGAGAAAA 
GGCCAGTGTT GTCCCTATTG CATAGAAGAC ACGATTCCGA AGAAGGTGGT GTGCCACTTC 
AGTGGGAAGG CCTACGCCGA CGAGGAGCGC TGGGACATTG ACAGCTGCAC ACACTGCTAC 
TGCCTGCAGG GCCAGACCCT CTGCTCGACC GTCAGCTGCC CCCCTCTGCC CTGTGTCGAG 
CCCATCAACG TGGAAGGCAG TTGCTGCCCA ATGTGTCCAG AAATGTATGT CCCAGAACCA 
ACCAATATAC CCATTGAGAA AACAAATCAC CGTGGCGACA TCGACCTGGA GGTTCCCTTG 
TGGCCTACAC CAAGTGAAAA TGACATCATC CATCTTCCCA GAGACATGGG TCACCTCCAG 
GTAGATTACA GAGATAACAA CAGGCTGCAT TCAGGCGAAG ATTCTTCACT GGACTCCATT 
GCCTCGGTTG TGGTTCCCAT CATCATCGGC CTCTCGATTA TAATAGCATT CCTGTTCATC 
AATCAGAAGA AACAGTGGAT ACCACTGCTT TGCTGGTATC GAACACCAAC TAAGCCTTCT 
TCCTTAAATA ATCAGCTGGT GTCTGTGGAC TGCAAGAAAG GAAGCAGAGT CCCAGTGGAC 
AGTTCACAGA GAATGCTAAG AATTGCTGAA CCAGATGCAA GATTCAGTGG CTTCTACAGC 
ATGCAAAAAC AGAACCACCT ACAGGCAGAT AATTTCTACC AGACAGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013975755.1
CDS	10..2880
Translation	MYLVAGGRGLAGCGHLPVSLLGLLLLLARSGTRALVCLPCDESKCEEPRSCPGSIVQGVCGCCYMCARQRNESCGGAYGLHGACDRGLRCVIRPPLNGDSITEYEVGVCEDENWDDDQLLGFEPCNENLVSGCNIINGKCECDTIRTCNNPFEFPRKDMCLSALKRIEVFSVDCSTVECPSVQQAVCPLDSYETQVRLTADGCCTLPTRCECLSGLCGFPVCEVGSTPRIVSRGDGTPGKCCDVFECVNETKPACVFNNVEYNDGDMFRMDNCRFCRCQGGVSICFTAQCGELNCERYYVPEGECCPVCEDPVYPFNNPAGCYANGQIRAHGDRWREDDCTFCQCINGEPHCVATACGQSCMNPVKVPGECCPVCEEPTYITIDPPACGELSNCTLTEKDCVYGFKLDHNGCRTCQCKNREELCSGLKRGCTLDCPFGFLTDAHNCEICQCRPRPKKCRPVICDKYCPFGYLKNKHGCNICRCKKCPELLCSKICPLGFQQDSHGCLICKCREVLASAGPPVLSGTCLSMDGHHHKNEESWHDGCRECYCHNGREMCALITCPVPACGNPTIHPGQCCPSCSDDFVVQKPELSTPSICHAPGGEYFVEGETWNIDSCTQCTCHSGRVLCETEVCPPLLCENPSRTQDSCCPQCTDEPLQPSSSHNDSVPSYCKNDEGDIFLAAESWKPDVCTSCVCMDSVISCYSESCPSVSCERPVLRKGQCCPYCIEDTIPKKVVCHFSGKAYADEERWDIDSCTHCYCLQGQTLCSTVSCPPLPCVEPINVEGSCCPMCPEMYVPEPTNIPIEKTNHRGDIDLEVPLWPTPSENDIIHLPRDMGHLQVDYRDNNRLHSGEDSSLDSIASVVVPIIIGLSIIIAFLFINQKKQWIPLLCWYRTPTKPSSLNNQLVSVDCKKGSRVPVDSSQRMLRIAEPDARFSGFYSMQKQNHLQADNFYQTV

Target ORF information:

RefSeq Version	XM_014120280.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120280.1

ORF Insert Sequence:

ATGTACTTGG TGGCGGGGGG CAGGGGGCTG GCCGGCTGCG GGCACCTCCC GGTCTCGCTG 
CTGGGGCTGC TGCTGCTGCT GGCGCGCTCC GGCACCCGGG CGCTGGTCTG CCTGCCCTGC 
GACGAGTCCA AGTGCGAGGA GCCCAGGAGC TGCCCCGGGA GCATCGTGCA GGGCGTCTGC 
GGCTGCTGCT ACATGTGCGC CCGCCAGAGG AACGAGAGCT GCGGCGGCGC CTACGGGCTC 
CACGGAGCCT GCGACCGGGG GCTGCGCTGT GTCATCCGCC CCCCGCTCAA TGGCGACTCC 
ATCACCGAGT ACGAAGTGGG CGTCTGCGAA GATGAGAACT GGGATGATGA CCAGCTGCTT 
GGTTTTGAAC CCTGCAATGA AAATCTTGTG TCTGGCTGCA ATATAATCAA TGGGAAATGC 
GAATGTGACA CCATTCGCAC CTGCAACAAT CCCTTTGAGT TTCCAAGGAA GGATATGTGC 
CTTTCAGCTT TGAAGAGGAT TGAAGTTTTC AGCGTGGACT GCAGCACCGT GGAGTGCCCC 
TCCGTTCAGC AGGCCGTGTG CCCTCTGGAC AGCTACGAGA CTCAAGTCAG ACTCACGGCG 
GACGGTTGCT GCACCCTGCC AACAAGATGC GAGTGTCTCT CTGGCTTATG TGGTTTCCCC 
GTGTGTGAGG TGGGATCCAC TCCTCGCATA GTCTCTCGTG GAGATGGGAC ACCTGGAAAG 
TGCTGTGATG TCTTTGAATG TGTTAATGAA ACAAAGCCAG CCTGTGTATT CAACAACGTG 
GAATATAACG ATGGGGATAT GTTTCGAATG GACAACTGTC GATTCTGTCG ATGCCAGGGG 
GGTGTTTCCA TCTGCTTCAC TGCCCAGTGT GGTGAGCTGA ACTGCGAGAG GTATTATGTG 
CCCGAAGGAG AGTGCTGCCC AGTGTGTGAA GATCCGGTGT ATCCTTTTAA TAATCCTGCT 
GGCTGCTATG CCAATGGTCA GATCCGCGCG CACGGGGACC GGTGGCGAGA AGACGACTGC 
ACTTTCTGCC AGTGTATCAA TGGAGAACCC CACTGTGTGG CCACGGCCTG TGGGCAGAGC 
TGCATGAACC CCGTCAAAGT GCCTGGCGAG TGTTGCCCTG TGTGTGAAGA GCCAACGTAC 
ATCACAATTG ATCCACCTGC ATGTGGGGAG TTATCAAACT GCACGCTGAC AGAGAAGGAC 
TGCGTTTATG GTTTCAAACT TGATCACAAT GGTTGTCGAA CCTGTCAGTG CAAAAACAGG 
GAGGAGCTGT GCTCGGGCCT CAAGCGAGGC TGTACTTTGG ACTGCCCCTT CGGCTTCCTG 
ACTGATGCCC ACAACTGTGA GATCTGTCAG TGCCGCCCAC GGCCCAAGAA ATGCAGACCT 
GTAATCTGTG ACAAGTATTG TCCATTTGGA TATCTGAAGA ATAAGCATGG CTGTAACATC 
TGTCGCTGCA AGAAATGCCC AGAGCTCCTG TGCAGTAAAA TTTGCCCCTT GGGTTTCCAG 
CAGGACAGTC ATGGCTGTCT TATCTGCAAG TGCAGAGAGG TCCTTGCTTC AGCCGGGCCA 
CCTGTCCTGT CAGGCACATG TCTGTCCATG GATGGCCATC ATCATAAAAA TGAGGAAAGC 
TGGCATGATG GGTGCCGGGA ATGCTACTGT CACAATGGAC GGGAAATGTG TGCTCTGATC 
ACCTGCCCGG TGCCTGCCTG TGGCAACCCC ACCATTCATC CCGGACAGTG CTGCCCGTCA 
TGTTCAGATG ACTTTGTGGT GCAAAAGCCA GAGCTCAGCA CCCCCTCCAT TTGCCATGCC 
CCTGGAGGAG AGTACTTCGT GGAAGGAGAG ACATGGAACA TCGACTCGTG TACTCAGTGC 
ACCTGCCACA GCGGGCGGGT GCTGTGTGAG ACGGAGGTGT GCCCACCGCT GCTCTGCGAG 
AACCCCTCGC GCACCCAGGA CTCCTGCTGC CCGCAGTGCA CAGATGAACC TCTTCAGCCT 
TCCTCATCCC ATAATGACAG TGTGCCTAGT TACTGCAAAA ATGACGAAGG GGATATATTC 
CTGGCAGCCG AGTCCTGGAA GCCTGACGTC TGTACCAGCT GTGTATGCAT GGATAGCGTA 
ATTAGCTGTT ACTCTGAATC CTGCCCTTCA GTATCCTGTG AAAGACCTGT TTTGAGAAAA 
GGCCAGTGTT GTCCCTATTG CATAGAAGAC ACGATTCCGA AGAAGGTGGT GTGCCACTTC 
AGTGGGAAGG CCTACGCCGA CGAGGAGCGC TGGGACATTG ACAGCTGCAC ACACTGCTAC 
TGCCTGCAGG GCCAGACCCT CTGCTCGACC GTCAGCTGCC CCCCTCTGCC CTGTGTCGAG 
CCCATCAACG TGGAAGGCAG TTGCTGCCCA ATGTGTCCAG AAATGTATGT CCCAGAACCA 
ACCAATATAC CCATTGAGAA AACAAATCAC CGTGGCGACA TCGACCTGGA GGTTCCCTTG 
TGGCCTACAC CAAGTGAAAA TGACATCATC CATCTTCCCA GAGACATGGG TCACCTCCAG 
GTAGATTACA GAGATAACAA CAGGCTGCAT TCAGGCGAAG ATTCTTCACT GGACTCCATT 
GCCTCGGTTG TGGTTCCCAT CATCATCGGC CTCTCGATTA TAATAGCATT CCTGTTCATC 
AATCAGAAGA AACAGTGGAT ACCACTGCTT TGCTGGTATC GAACACCAAC TAAGCCTTCT 
TCCTTAAATA ATCAGCTGGT GTCTGTGGAC TGCAAGAAAG GAAGCAGAGT CCCAGTGGAC 
AGTTCACAGA GAATGCTAAG AATTGCTGAA CCAGATGCAA GATTCAGTGG CTTCTACAGC 
ATGCAAAAAC AGAACCACCT ACAGGCAGAT AATTTCTACC AGACAGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRIM1 ( XM_014120281.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013975756.1 Canis lupus familiaris cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe40693
Clone ID Related Accession (Same CDS sequence)	XM_014120281.1
Accession Version	XM_014120281.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	cysteine-rich motor neuron 1 protein isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTTGG TGGCGGGGGG CAGGGGGCTG GCCGGCTGCG GGCACCTCCC GGTCTCGCTG 
CTGGGGCTGC TGCTGCTGCT GGCGCGCTCC GGCACCCGGG CGCTGGTCTG CCTGCCCTGC 
GACGAGTCCA AGTGCGAGGA GCCCAGGAGC TGCCCCGGGA GCATCGTGCA GGGCGTCTGC 
GGCTGCTGCT ACATGTGCGC CCGCCAGAGG AACGAGAGCT GCGGCGGCGC CTACGGGCTC 
CACGGAGCCT GCGACCGGGG GCTGCGCTGT GTCATCCGCC CCCCGCTCAA TGGCGACTCC 
ATCACCGAGT ACGAAGTGGG CGTCTGCGAA GATGAGAACT GGGATGATGA CCAGCTGCTT 
GGTTTTGAAC CCTGCAATGA AAATCTTGTG TCTGGCTGCA ATATAATCAA TGGGAAATGC 
GAATGTGACA CCATTCGCAC CTGCAACAAT CCCTTTGAGT TTCCAAGGAA GGATATGTGC 
CTTTCAGCTT TGAAGAGGAT TGAAGAAACA AAGCCAGCCT GTGTATTCAA CAACGTGGAA 
TATAACGATG GGGATATGTT TCGAATGGAC AACTGTCGAT TCTGTCGATG CCAGGGGGGT 
GTTTCCATCT GCTTCACTGC CCAGTGTGGT GAGCTGAACT GCGAGAGGTA TTATGTGCCC 
GAAGGAGAGT GCTGCCCAGT GTGTGAAGAT CCGGTGTATC CTTTTAATAA TCCTGCTGGC 
TGCTATGCCA ATGGTCAGAT CCGCGCGCAC GGGGACCGGT GGCGAGAAGA CGACTGCACT 
TTCTGCCAGT GTATCAATGG AGAACCCCAC TGTGTGGCCA CGGCCTGTGG GCAGAGCTGC 
ATGAACCCCG TCAAAGTGCC TGGCGAGTGT TGCCCTGTGT GTGAAGAGCC AACGTACATC 
ACAATTGATC CACCTGCATG TGGGGAGTTA TCAAACTGCA CGCTGACAGA GAAGGACTGC 
GTTTATGGTT TCAAACTTGA TCACAATGGT TGTCGAACCT GTCAGTGCAA AAACAGGGAG 
GAGCTGTGCT CGGGCCTCAA GCGAGGCTGT ACTTTGGACT GCCCCTTCGG CTTCCTGACT 
GATGCCCACA ACTGTGAGAT CTGTCAGTGC CGCCCACGGC CCAAGAAATG CAGACCTGTA 
ATCTGTGACA AGTATTGTCC ATTTGGATAT CTGAAGAATA AGCATGGCTG TAACATCTGT 
CGCTGCAAGA AATGCCCAGA GCTCCTGTGC AGTAAAATTT GCCCCTTGGG TTTCCAGCAG 
GACAGTCATG GCTGTCTTAT CTGCAAGTGC AGAGAGGTCC TTGCTTCAGC CGGGCCACCT 
GTCCTGTCAG GCACATGTCT GTCCATGGAT GGCCATCATC ATAAAAATGA GGAAAGCTGG 
CATGATGGGT GCCGGGAATG CTACTGTCAC AATGGACGGG AAATGTGTGC TCTGATCACC 
TGCCCGGTGC CTGCCTGTGG CAACCCCACC ATTCATCCCG GACAGTGCTG CCCGTCATGT 
TCAGATGACT TTGTGGTGCA AAAGCCAGAG CTCAGCACCC CCTCCATTTG CCATGCCCCT 
GGAGGAGAGT ACTTCGTGGA AGGAGAGACA TGGAACATCG ACTCGTGTAC TCAGTGCACC 
TGCCACAGCG GGCGGGTGCT GTGTGAGACG GAGGTGTGCC CACCGCTGCT CTGCGAGAAC 
CCCTCGCGCA CCCAGGACTC CTGCTGCCCG CAGTGCACAG ATGAACCTCT TCAGCCTTCC 
TCATCCCATA ATGACAGTGT GCCTAGTTAC TGCAAAAATG ACGAAGGGGA TATATTCCTG 
GCAGCCGAGT CCTGGAAGCC TGACGTCTGT ACCAGCTGTG TATGCATGGA TAGCGTAATT 
AGCTGTTACT CTGAATCCTG CCCTTCAGTA TCCTGTGAAA GACCTGTTTT GAGAAAAGGC 
CAGTGTTGTC CCTATTGCAT AGAAGACACG ATTCCGAAGA AGGTGGTGTG CCACTTCAGT 
GGGAAGGCCT ACGCCGACGA GGAGCGCTGG GACATTGACA GCTGCACACA CTGCTACTGC 
CTGCAGGGCC AGACCCTCTG CTCGACCGTC AGCTGCCCCC CTCTGCCCTG TGTCGAGCCC 
ATCAACGTGG AAGGCAGTTG CTGCCCAATG TGTCCAGAAA TGTATGTCCC AGAACCAACC 
AATATACCCA TTGAGAAAAC AAATCACCGT GGCGACATCG ACCTGGAGGT TCCCTTGTGG 
CCTACACCAA GTGAAAATGA CATCATCCAT CTTCCCAGAG ACATGGGTCA CCTCCAGGTA 
GATTACAGAG ATAACAACAG GCTGCATTCA GGCGAAGATT CTTCACTGGA CTCCATTGCC 
TCGGTTGTGG TTCCCATCAT CATCGGCCTC TCGATTATAA TAGCATTCCT GTTCATCAAT 
CAGAAGAAAC AGTGGATACC ACTGCTTTGC TGGTATCGAA CACCAACTAA GCCTTCTTCC 
TTAAATAATC AGCTGGTGTC TGTGGACTGC AAGAAAGGAA GCAGAGTCCC AGTGGACAGT 
TCACAGAGAA TGCTAAGAAT TGCTGAACCA GATGCAAGAT TCAGTGGCTT CTACAGCATG 
CAAAAACAGA ACCACCTACA GGCAGATAAT TTCTACCAGA CAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013975756.1
CDS	10..2637
Translation	MYLVAGGRGLAGCGHLPVSLLGLLLLLARSGTRALVCLPCDESKCEEPRSCPGSIVQGVCGCCYMCARQRNESCGGAYGLHGACDRGLRCVIRPPLNGDSITEYEVGVCEDENWDDDQLLGFEPCNENLVSGCNIINGKCECDTIRTCNNPFEFPRKDMCLSALKRIEETKPACVFNNVEYNDGDMFRMDNCRFCRCQGGVSICFTAQCGELNCERYYVPEGECCPVCEDPVYPFNNPAGCYANGQIRAHGDRWREDDCTFCQCINGEPHCVATACGQSCMNPVKVPGECCPVCEEPTYITIDPPACGELSNCTLTEKDCVYGFKLDHNGCRTCQCKNREELCSGLKRGCTLDCPFGFLTDAHNCEICQCRPRPKKCRPVICDKYCPFGYLKNKHGCNICRCKKCPELLCSKICPLGFQQDSHGCLICKCREVLASAGPPVLSGTCLSMDGHHHKNEESWHDGCRECYCHNGREMCALITCPVPACGNPTIHPGQCCPSCSDDFVVQKPELSTPSICHAPGGEYFVEGETWNIDSCTQCTCHSGRVLCETEVCPPLLCENPSRTQDSCCPQCTDEPLQPSSSHNDSVPSYCKNDEGDIFLAAESWKPDVCTSCVCMDSVISCYSESCPSVSCERPVLRKGQCCPYCIEDTIPKKVVCHFSGKAYADEERWDIDSCTHCYCLQGQTLCSTVSCPPLPCVEPINVEGSCCPMCPEMYVPEPTNIPIEKTNHRGDIDLEVPLWPTPSENDIIHLPRDMGHLQVDYRDNNRLHSGEDSSLDSIASVVVPIIIGLSIIIAFLFINQKKQWIPLLCWYRTPTKPSSLNNQLVSVDCKKGSRVPVDSSQRMLRIAEPDARFSGFYSMQKQNHLQADNFYQTV

Target ORF information:

RefSeq Version	XM_014120281.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120281.1

ORF Insert Sequence:

ATGTACTTGG TGGCGGGGGG CAGGGGGCTG GCCGGCTGCG GGCACCTCCC GGTCTCGCTG 
CTGGGGCTGC TGCTGCTGCT GGCGCGCTCC GGCACCCGGG CGCTGGTCTG CCTGCCCTGC 
GACGAGTCCA AGTGCGAGGA GCCCAGGAGC TGCCCCGGGA GCATCGTGCA GGGCGTCTGC 
GGCTGCTGCT ACATGTGCGC CCGCCAGAGG AACGAGAGCT GCGGCGGCGC CTACGGGCTC 
CACGGAGCCT GCGACCGGGG GCTGCGCTGT GTCATCCGCC CCCCGCTCAA TGGCGACTCC 
ATCACCGAGT ACGAAGTGGG CGTCTGCGAA GATGAGAACT GGGATGATGA CCAGCTGCTT 
GGTTTTGAAC CCTGCAATGA AAATCTTGTG TCTGGCTGCA ATATAATCAA TGGGAAATGC 
GAATGTGACA CCATTCGCAC CTGCAACAAT CCCTTTGAGT TTCCAAGGAA GGATATGTGC 
CTTTCAGCTT TGAAGAGGAT TGAAGAAACA AAGCCAGCCT GTGTATTCAA CAACGTGGAA 
TATAACGATG GGGATATGTT TCGAATGGAC AACTGTCGAT TCTGTCGATG CCAGGGGGGT 
GTTTCCATCT GCTTCACTGC CCAGTGTGGT GAGCTGAACT GCGAGAGGTA TTATGTGCCC 
GAAGGAGAGT GCTGCCCAGT GTGTGAAGAT CCGGTGTATC CTTTTAATAA TCCTGCTGGC 
TGCTATGCCA ATGGTCAGAT CCGCGCGCAC GGGGACCGGT GGCGAGAAGA CGACTGCACT 
TTCTGCCAGT GTATCAATGG AGAACCCCAC TGTGTGGCCA CGGCCTGTGG GCAGAGCTGC 
ATGAACCCCG TCAAAGTGCC TGGCGAGTGT TGCCCTGTGT GTGAAGAGCC AACGTACATC 
ACAATTGATC CACCTGCATG TGGGGAGTTA TCAAACTGCA CGCTGACAGA GAAGGACTGC 
GTTTATGGTT TCAAACTTGA TCACAATGGT TGTCGAACCT GTCAGTGCAA AAACAGGGAG 
GAGCTGTGCT CGGGCCTCAA GCGAGGCTGT ACTTTGGACT GCCCCTTCGG CTTCCTGACT 
GATGCCCACA ACTGTGAGAT CTGTCAGTGC CGCCCACGGC CCAAGAAATG CAGACCTGTA 
ATCTGTGACA AGTATTGTCC ATTTGGATAT CTGAAGAATA AGCATGGCTG TAACATCTGT 
CGCTGCAAGA AATGCCCAGA GCTCCTGTGC AGTAAAATTT GCCCCTTGGG TTTCCAGCAG 
GACAGTCATG GCTGTCTTAT CTGCAAGTGC AGAGAGGTCC TTGCTTCAGC CGGGCCACCT 
GTCCTGTCAG GCACATGTCT GTCCATGGAT GGCCATCATC ATAAAAATGA GGAAAGCTGG 
CATGATGGGT GCCGGGAATG CTACTGTCAC AATGGACGGG AAATGTGTGC TCTGATCACC 
TGCCCGGTGC CTGCCTGTGG CAACCCCACC ATTCATCCCG GACAGTGCTG CCCGTCATGT 
TCAGATGACT TTGTGGTGCA AAAGCCAGAG CTCAGCACCC CCTCCATTTG CCATGCCCCT 
GGAGGAGAGT ACTTCGTGGA AGGAGAGACA TGGAACATCG ACTCGTGTAC TCAGTGCACC 
TGCCACAGCG GGCGGGTGCT GTGTGAGACG GAGGTGTGCC CACCGCTGCT CTGCGAGAAC 
CCCTCGCGCA CCCAGGACTC CTGCTGCCCG CAGTGCACAG ATGAACCTCT TCAGCCTTCC 
TCATCCCATA ATGACAGTGT GCCTAGTTAC TGCAAAAATG ACGAAGGGGA TATATTCCTG 
GCAGCCGAGT CCTGGAAGCC TGACGTCTGT ACCAGCTGTG TATGCATGGA TAGCGTAATT 
AGCTGTTACT CTGAATCCTG CCCTTCAGTA TCCTGTGAAA GACCTGTTTT GAGAAAAGGC 
CAGTGTTGTC CCTATTGCAT AGAAGACACG ATTCCGAAGA AGGTGGTGTG CCACTTCAGT 
GGGAAGGCCT ACGCCGACGA GGAGCGCTGG GACATTGACA GCTGCACACA CTGCTACTGC 
CTGCAGGGCC AGACCCTCTG CTCGACCGTC AGCTGCCCCC CTCTGCCCTG TGTCGAGCCC 
ATCAACGTGG AAGGCAGTTG CTGCCCAATG TGTCCAGAAA TGTATGTCCC AGAACCAACC 
AATATACCCA TTGAGAAAAC AAATCACCGT GGCGACATCG ACCTGGAGGT TCCCTTGTGG 
CCTACACCAA GTGAAAATGA CATCATCCAT CTTCCCAGAG ACATGGGTCA CCTCCAGGTA 
GATTACAGAG ATAACAACAG GCTGCATTCA GGCGAAGATT CTTCACTGGA CTCCATTGCC 
TCGGTTGTGG TTCCCATCAT CATCGGCCTC TCGATTATAA TAGCATTCCT GTTCATCAAT 
CAGAAGAAAC AGTGGATACC ACTGCTTTGC TGGTATCGAA CACCAACTAA GCCTTCTTCC 
TTAAATAATC AGCTGGTGTC TGTGGACTGC AAGAAAGGAA GCAGAGTCCC AGTGGACAGT 
TCACAGAGAA TGCTAAGAAT TGCTGAACCA GATGCAAGAT TCAGTGGCTT CTACAGCATG 
CAAAAACAGA ACCACCTACA GGCAGATAAT TTCTACCAGA CAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRIM1 ( XM_532931.5 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_532931.2 Canis lupus familiaris cysteine rich transmembrane BMP regulator 1 (CRIM1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe18878
Clone ID Related Accession (Same CDS sequence)	XM_532931.5 , XM_532931.4
Accession Version	XM_532931.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3114bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	cysteine-rich motor neuron 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_532931.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTTGG TGGCGGGGGG CAGGGGGCTG GCCGGCTGCG GGCACCTCCC GGTCTCGCTG 
CTGGGGCTGC TGCTGCTGCT GGCGCGCTCC GGCACCCGGG CGCTGGTCTG CCTGCCCTGC 
GACGAGTCCA AGTGCGAGGA GCCCAGGAGC TGCCCCGGGA GCATCGTGCA GGGCGTCTGC 
GGCTGCTGCT ACATGTGCGC CCGCCAGAGG AACGAGAGCT GCGGCGGCGC CTACGGGCTC 
CACGGAGCCT GCGACCGGGG GCTGCGCTGT GTCATCCGCC CCCCGCTCAA TGGCGACTCC 
ATCACCGAGT ACGAAGTGGG CGTCTGCGAA GATGAGAACT GGGATGATGA CCAGCTGCTT 
GGTTTTGAAC CCTGCAATGA AAATCTTGTG TCTGGCTGCA ATATAATCAA TGGGAAATGC 
GAATGTGACA CCATTCGCAC CTGCAACAAT CCCTTTGAGT TTCCAAGGAA GGATATGTGC 
CTTTCAGCTT TGAAGAGGAT TGAAGAAGAG AAGCCAGATT GCTCCAAGGC CCGCTGTGAA 
GTCCAGTTCT CTCCACGTTG TCCTGAAGAT TCCGTTCTGA TCGAGGGCTA TGCTCCCCCC 
GGGGAGTGCT GCCCCTTACC CAGCCGCTGC GTGTGCGACC CTGCGGGCTG TCTGCGCAAA 
GTCTGCCAGC CGGGATACCT GAACATACTA GTGTCAAAGG CCTCAGGGAA GCCGGGAGAG 
TGTTGTGACC TCTATGAGTG CAAACCAGTT TTCAGCGTGG ACTGCAGCAC CGTGGAGTGC 
CCCTCCGTTC AGCAGGCCGT GTGCCCTCTG GACAGCTACG AGACTCAAGT CAGACTCACG 
GCGGACGGTT GCTGCACCCT GCCAACAAGA TGCGAGTGTC TCTCTGGCTT ATGTGGTTTC 
CCCGTGTGTG AGGTGGGATC CACTCCTCGC ATAGTCTCTC GTGGAGATGG GACACCTGGA 
AAGTGCTGTG ATGTCTTTGA ATGTGTTAAT GAAACAAAGC CAGCCTGTGT ATTCAACAAC 
GTGGAATATA ACGATGGGGA TATGTTTCGA ATGGACAACT GTCGATTCTG TCGATGCCAG 
GGGGGTGTTT CCATCTGCTT CACTGCCCAG TGTGGTGAGC TGAACTGCGA GAGGTATTAT 
GTGCCCGAAG GAGAGTGCTG CCCAGTGTGT GAAGATCCGG TGTATCCTTT TAATAATCCT 
GCTGGCTGCT ATGCCAATGG TCAGATCCGC GCGCACGGGG ACCGGTGGCG AGAAGACGAC 
TGCACTTTCT GCCAGTGTAT CAATGGAGAA CCCCACTGTG TGGCCACGGC CTGTGGGCAG 
AGCTGCATGA ACCCCGTCAA AGTGCCTGGC GAGTGTTGCC CTGTGTGTGA AGAGCCAACG 
TACATCACAA TTGATCCACC TGCATGTGGG GAGTTATCAA ACTGCACGCT GACAGAGAAG 
GACTGCGTTT ATGGTTTCAA ACTTGATCAC AATGGTTGTC GAACCTGTCA GTGCAAAAAC 
AGGGAGGAGC TGTGCTCGGG CCTCAAGCGA GGCTGTACTT TGGACTGCCC CTTCGGCTTC 
CTGACTGATG CCCACAACTG TGAGATCTGT CAGTGCCGCC CACGGCCCAA GAAATGCAGA 
CCTGTAATCT GTGACAAGTA TTGTCCATTT GGATATCTGA AGAATAAGCA TGGCTGTAAC 
ATCTGTCGCT GCAAGAAATG CCCAGAGCTC CTGTGCAGTA AAATTTGCCC CTTGGGTTTC 
CAGCAGGACA GTCATGGCTG TCTTATCTGC AAGTGCAGAG AGGTCCTTGC TTCAGCCGGG 
CCACCTGTCC TGTCAGGCAC ATGTCTGTCC ATGGATGGCC ATCATCATAA AAATGAGGAA 
AGCTGGCATG ATGGGTGCCG GGAATGCTAC TGTCACAATG GACGGGAAAT GTGTGCTCTG 
ATCACCTGCC CGGTGCCTGC CTGTGGCAAC CCCACCATTC ATCCCGGACA GTGCTGCCCG 
TCATGTTCAG ATGACTTTGT GGTGCAAAAG CCAGAGCTCA GCACCCCCTC CATTTGCCAT 
GCCCCTGGAG GAGAGTACTT CGTGGAAGGA GAGACATGGA ACATCGACTC GTGTACTCAG 
TGCACCTGCC ACAGCGGGCG GGTGCTGTGT GAGACGGAGG TGTGCCCACC GCTGCTCTGC 
GAGAACCCCT CGCGCACCCA GGACTCCTGC TGCCCGCAGT GCACAGATGA ACCTCTTCAG 
CCTTCCTCAT CCCATAATGA CAGTGTGCCT AGTTACTGCA AAAATGACGA AGGGGATATA 
TTCCTGGCAG CCGAGTCCTG GAAGCCTGAC GTCTGTACCA GCTGTGTATG CATGGATAGC 
GTAATTAGCT GTTACTCTGA ATCCTGCCCT TCAGTATCCT GTGAAAGACC TGTTTTGAGA 
AAAGGCCAGT GTTGTCCCTA TTGCATAGAA GACACGATTC CGAAGAAGGT GGTGTGCCAC 
TTCAGTGGGA AGGCCTACGC CGACGAGGAG CGCTGGGACA TTGACAGCTG CACACACTGC 
TACTGCCTGC AGGGCCAGAC CCTCTGCTCG ACCGTCAGCT GCCCCCCTCT GCCCTGTGTC 
GAGCCCATCA ACGTGGAAGG CAGTTGCTGC CCAATGTGTC CAGAAATGTA TGTCCCAGAA 
CCAACCAATA TACCCATTGA GAAAACAAAT CACCGTGGCG ACATCGACCT GGAGGTTCCC 
TTGTGGCCTA CACCAAGTGA AAATGACATC ATCCATCTTC CCAGAGACAT GGGTCACCTC 
CAGGTAGATT ACAGAGATAA CAACAGGCTG CATTCAGGCG AAGATTCTTC ACTGGACTCC 
ATTGCCTCGG TTGTGGTTCC CATCATCATC GGCCTCTCGA TTATAATAGC ATTCCTGTTC 
ATCAATCAGA AGAAACAGTG GATACCACTG CTTTGCTGGT ATCGAACACC AACTAAGCCT 
TCTTCCTTAA ATAATCAGCT GGTGTCTGTG GACTGCAAGA AAGGAAGCAG AGTCCCAGTG 
GACAGTTCAC AGAGAATGCT AAGAATTGCT GAACCAGATG CAAGATTCAG TGGCTTCTAC 
AGCATGCAAA AACAGAACCA CCTACAGGCA GATAATTTCT ACCAGACAGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_532931.2
CDS	10..3123
Translation	MYLVAGGRGLAGCGHLPVSLLGLLLLLARSGTRALVCLPCDESKCEEPRSCPGSIVQGVCGCCYMCARQRNESCGGAYGLHGACDRGLRCVIRPPLNGDSITEYEVGVCEDENWDDDQLLGFEPCNENLVSGCNIINGKCECDTIRTCNNPFEFPRKDMCLSALKRIEEEKPDCSKARCEVQFSPRCPEDSVLIEGYAPPGECCPLPSRCVCDPAGCLRKVCQPGYLNILVSKASGKPGECCDLYECKPVFSVDCSTVECPSVQQAVCPLDSYETQVRLTADGCCTLPTRCECLSGLCGFPVCEVGSTPRIVSRGDGTPGKCCDVFECVNETKPACVFNNVEYNDGDMFRMDNCRFCRCQGGVSICFTAQCGELNCERYYVPEGECCPVCEDPVYPFNNPAGCYANGQIRAHGDRWREDDCTFCQCINGEPHCVATACGQSCMNPVKVPGECCPVCEEPTYITIDPPACGELSNCTLTEKDCVYGFKLDHNGCRTCQCKNREELCSGLKRGCTLDCPFGFLTDAHNCEICQCRPRPKKCRPVICDKYCPFGYLKNKHGCNICRCKKCPELLCSKICPLGFQQDSHGCLICKCREVLASAGPPVLSGTCLSMDGHHHKNEESWHDGCRECYCHNGREMCALITCPVPACGNPTIHPGQCCPSCSDDFVVQKPELSTPSICHAPGGEYFVEGETWNIDSCTQCTCHSGRVLCETEVCPPLLCENPSRTQDSCCPQCTDEPLQPSSSHNDSVPSYCKNDEGDIFLAAESWKPDVCTSCVCMDSVISCYSESCPSVSCERPVLRKGQCCPYCIEDTIPKKVVCHFSGKAYADEERWDIDSCTHCYCLQGQTLCSTVSCPPLPCVEPINVEGSCCPMCPEMYVPEPTNIPIEKTNHRGDIDLEVPLWPTPSENDIIHLPRDMGHLQVDYRDNNRLHSGEDSSLDSIASVVVPIIIGLSIIIAFLFINQKKQWIPLLCWYRTPTKPSSLNNQLVSVDCKKGSRVPVDSSQRMLRIAEPDARFSGFYSMQKQNHLQADNFYQTV

Target ORF information:

RefSeq Version	XM_532931.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris cysteine rich transmembrane BMP regulator 1 (CRIM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_532931.5

ORF Insert Sequence:

ATGTACTTGG TGGCGGGGGG CAGGGGGCTG GCCGGCTGCG GGCACCTCCC GGTCTCGCTG 
CTGGGGCTGC TGCTGCTGCT GGCGCGCTCC GGCACCCGGG CGCTGGTCTG CCTGCCCTGC 
GACGAGTCCA AGTGCGAGGA GCCCAGGAGC TGCCCCGGGA GCATCGTGCA GGGCGTCTGC 
GGCTGCTGCT ACATGTGCGC CCGCCAGAGG AACGAGAGCT GCGGCGGCGC CTACGGGCTC 
CACGGAGCCT GCGACCGGGG GCTGCGCTGT GTCATCCGCC CCCCGCTCAA TGGCGACTCC 
ATCACCGAGT ACGAAGTGGG CGTCTGCGAA GATGAGAACT GGGATGATGA CCAGCTGCTT 
GGTTTTGAAC CCTGCAATGA AAATCTTGTG TCTGGCTGCA ATATAATCAA TGGGAAATGC 
GAATGTGACA CCATTCGCAC CTGCAACAAT CCCTTTGAGT TTCCAAGGAA GGATATGTGC 
CTTTCAGCTT TGAAGAGGAT TGAAGAAGAG AAGCCAGATT GCTCCAAGGC CCGCTGTGAA 
GTCCAGTTCT CTCCACGTTG TCCTGAAGAT TCCGTTCTGA TCGAGGGCTA TGCTCCCCCC 
GGGGAGTGCT GCCCCTTACC CAGCCGCTGC GTGTGCGACC CTGCGGGCTG TCTGCGCAAA 
GTCTGCCAGC CGGGATACCT GAACATACTA GTGTCAAAGG CCTCAGGGAA GCCGGGAGAG 
TGTTGTGACC TCTATGAGTG CAAACCAGTT TTCAGCGTGG ACTGCAGCAC CGTGGAGTGC 
CCCTCCGTTC AGCAGGCCGT GTGCCCTCTG GACAGCTACG AGACTCAAGT CAGACTCACG 
GCGGACGGTT GCTGCACCCT GCCAACAAGA TGCGAGTGTC TCTCTGGCTT ATGTGGTTTC 
CCCGTGTGTG AGGTGGGATC CACTCCTCGC ATAGTCTCTC GTGGAGATGG GACACCTGGA 
AAGTGCTGTG ATGTCTTTGA ATGTGTTAAT GAAACAAAGC CAGCCTGTGT ATTCAACAAC 
GTGGAATATA ACGATGGGGA TATGTTTCGA ATGGACAACT GTCGATTCTG TCGATGCCAG 
GGGGGTGTTT CCATCTGCTT CACTGCCCAG TGTGGTGAGC TGAACTGCGA GAGGTATTAT 
GTGCCCGAAG GAGAGTGCTG CCCAGTGTGT GAAGATCCGG TGTATCCTTT TAATAATCCT 
GCTGGCTGCT ATGCCAATGG TCAGATCCGC GCGCACGGGG ACCGGTGGCG AGAAGACGAC 
TGCACTTTCT GCCAGTGTAT CAATGGAGAA CCCCACTGTG TGGCCACGGC CTGTGGGCAG 
AGCTGCATGA ACCCCGTCAA AGTGCCTGGC GAGTGTTGCC CTGTGTGTGA AGAGCCAACG 
TACATCACAA TTGATCCACC TGCATGTGGG GAGTTATCAA ACTGCACGCT GACAGAGAAG 
GACTGCGTTT ATGGTTTCAA ACTTGATCAC AATGGTTGTC GAACCTGTCA GTGCAAAAAC 
AGGGAGGAGC TGTGCTCGGG CCTCAAGCGA GGCTGTACTT TGGACTGCCC CTTCGGCTTC 
CTGACTGATG CCCACAACTG TGAGATCTGT CAGTGCCGCC CACGGCCCAA GAAATGCAGA 
CCTGTAATCT GTGACAAGTA TTGTCCATTT GGATATCTGA AGAATAAGCA TGGCTGTAAC 
ATCTGTCGCT GCAAGAAATG CCCAGAGCTC CTGTGCAGTA AAATTTGCCC CTTGGGTTTC 
CAGCAGGACA GTCATGGCTG TCTTATCTGC AAGTGCAGAG AGGTCCTTGC TTCAGCCGGG 
CCACCTGTCC TGTCAGGCAC ATGTCTGTCC ATGGATGGCC ATCATCATAA AAATGAGGAA 
AGCTGGCATG ATGGGTGCCG GGAATGCTAC TGTCACAATG GACGGGAAAT GTGTGCTCTG 
ATCACCTGCC CGGTGCCTGC CTGTGGCAAC CCCACCATTC ATCCCGGACA GTGCTGCCCG 
TCATGTTCAG ATGACTTTGT GGTGCAAAAG CCAGAGCTCA GCACCCCCTC CATTTGCCAT 
GCCCCTGGAG GAGAGTACTT CGTGGAAGGA GAGACATGGA ACATCGACTC GTGTACTCAG 
TGCACCTGCC ACAGCGGGCG GGTGCTGTGT GAGACGGAGG TGTGCCCACC GCTGCTCTGC 
GAGAACCCCT CGCGCACCCA GGACTCCTGC TGCCCGCAGT GCACAGATGA ACCTCTTCAG 
CCTTCCTCAT CCCATAATGA CAGTGTGCCT AGTTACTGCA AAAATGACGA AGGGGATATA 
TTCCTGGCAG CCGAGTCCTG GAAGCCTGAC GTCTGTACCA GCTGTGTATG CATGGATAGC 
GTAATTAGCT GTTACTCTGA ATCCTGCCCT TCAGTATCCT GTGAAAGACC TGTTTTGAGA 
AAAGGCCAGT GTTGTCCCTA TTGCATAGAA GACACGATTC CGAAGAAGGT GGTGTGCCAC 
TTCAGTGGGA AGGCCTACGC CGACGAGGAG CGCTGGGACA TTGACAGCTG CACACACTGC 
TACTGCCTGC AGGGCCAGAC CCTCTGCTCG ACCGTCAGCT GCCCCCCTCT GCCCTGTGTC 
GAGCCCATCA ACGTGGAAGG CAGTTGCTGC CCAATGTGTC CAGAAATGTA TGTCCCAGAA 
CCAACCAATA TACCCATTGA GAAAACAAAT CACCGTGGCG ACATCGACCT GGAGGTTCCC 
TTGTGGCCTA CACCAAGTGA AAATGACATC ATCCATCTTC CCAGAGACAT GGGTCACCTC 
CAGGTAGATT ACAGAGATAA CAACAGGCTG CATTCAGGCG AAGATTCTTC ACTGGACTCC 
ATTGCCTCGG TTGTGGTTCC CATCATCATC GGCCTCTCGA TTATAATAGC ATTCCTGTTC 
ATCAATCAGA AGAAACAGTG GATACCACTG CTTTGCTGGT ATCGAACACC AACTAAGCCT 
TCTTCCTTAA ATAATCAGCT GGTGTCTGTG GACTGCAAGA AAGGAAGCAG AGTCCCAGTG 
GACAGTTCAC AGAGAATGCT AAGAATTGCT GAACCAGATG CAAGATTCAG TGGCTTCTAC 
AGCATGCAAA AACAGAACCA CCTACAGGCA GATAATTTCT ACCAGACAGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRIM1 ( XM_532931.4 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_532931.2 Canis lupus familiaris cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe18878
Clone ID Related Accession (Same CDS sequence)	XM_532931.5 , XM_532931.4
Accession Version	XM_532931.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3114bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	cysteine-rich motor neuron 1 protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_532931.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTTGG TGGCGGGGGG CAGGGGGCTG GCCGGCTGCG GGCACCTCCC GGTCTCGCTG 
CTGGGGCTGC TGCTGCTGCT GGCGCGCTCC GGCACCCGGG CGCTGGTCTG CCTGCCCTGC 
GACGAGTCCA AGTGCGAGGA GCCCAGGAGC TGCCCCGGGA GCATCGTGCA GGGCGTCTGC 
GGCTGCTGCT ACATGTGCGC CCGCCAGAGG AACGAGAGCT GCGGCGGCGC CTACGGGCTC 
CACGGAGCCT GCGACCGGGG GCTGCGCTGT GTCATCCGCC CCCCGCTCAA TGGCGACTCC 
ATCACCGAGT ACGAAGTGGG CGTCTGCGAA GATGAGAACT GGGATGATGA CCAGCTGCTT 
GGTTTTGAAC CCTGCAATGA AAATCTTGTG TCTGGCTGCA ATATAATCAA TGGGAAATGC 
GAATGTGACA CCATTCGCAC CTGCAACAAT CCCTTTGAGT TTCCAAGGAA GGATATGTGC 
CTTTCAGCTT TGAAGAGGAT TGAAGAAGAG AAGCCAGATT GCTCCAAGGC CCGCTGTGAA 
GTCCAGTTCT CTCCACGTTG TCCTGAAGAT TCCGTTCTGA TCGAGGGCTA TGCTCCCCCC 
GGGGAGTGCT GCCCCTTACC CAGCCGCTGC GTGTGCGACC CTGCGGGCTG TCTGCGCAAA 
GTCTGCCAGC CGGGATACCT GAACATACTA GTGTCAAAGG CCTCAGGGAA GCCGGGAGAG 
TGTTGTGACC TCTATGAGTG CAAACCAGTT TTCAGCGTGG ACTGCAGCAC CGTGGAGTGC 
CCCTCCGTTC AGCAGGCCGT GTGCCCTCTG GACAGCTACG AGACTCAAGT CAGACTCACG 
GCGGACGGTT GCTGCACCCT GCCAACAAGA TGCGAGTGTC TCTCTGGCTT ATGTGGTTTC 
CCCGTGTGTG AGGTGGGATC CACTCCTCGC ATAGTCTCTC GTGGAGATGG GACACCTGGA 
AAGTGCTGTG ATGTCTTTGA ATGTGTTAAT GAAACAAAGC CAGCCTGTGT ATTCAACAAC 
GTGGAATATA ACGATGGGGA TATGTTTCGA ATGGACAACT GTCGATTCTG TCGATGCCAG 
GGGGGTGTTT CCATCTGCTT CACTGCCCAG TGTGGTGAGC TGAACTGCGA GAGGTATTAT 
GTGCCCGAAG GAGAGTGCTG CCCAGTGTGT GAAGATCCGG TGTATCCTTT TAATAATCCT 
GCTGGCTGCT ATGCCAATGG TCAGATCCGC GCGCACGGGG ACCGGTGGCG AGAAGACGAC 
TGCACTTTCT GCCAGTGTAT CAATGGAGAA CCCCACTGTG TGGCCACGGC CTGTGGGCAG 
AGCTGCATGA ACCCCGTCAA AGTGCCTGGC GAGTGTTGCC CTGTGTGTGA AGAGCCAACG 
TACATCACAA TTGATCCACC TGCATGTGGG GAGTTATCAA ACTGCACGCT GACAGAGAAG 
GACTGCGTTT ATGGTTTCAA ACTTGATCAC AATGGTTGTC GAACCTGTCA GTGCAAAAAC 
AGGGAGGAGC TGTGCTCGGG CCTCAAGCGA GGCTGTACTT TGGACTGCCC CTTCGGCTTC 
CTGACTGATG CCCACAACTG TGAGATCTGT CAGTGCCGCC CACGGCCCAA GAAATGCAGA 
CCTGTAATCT GTGACAAGTA TTGTCCATTT GGATATCTGA AGAATAAGCA TGGCTGTAAC 
ATCTGTCGCT GCAAGAAATG CCCAGAGCTC CTGTGCAGTA AAATTTGCCC CTTGGGTTTC 
CAGCAGGACA GTCATGGCTG TCTTATCTGC AAGTGCAGAG AGGTCCTTGC TTCAGCCGGG 
CCACCTGTCC TGTCAGGCAC ATGTCTGTCC ATGGATGGCC ATCATCATAA AAATGAGGAA 
AGCTGGCATG ATGGGTGCCG GGAATGCTAC TGTCACAATG GACGGGAAAT GTGTGCTCTG 
ATCACCTGCC CGGTGCCTGC CTGTGGCAAC CCCACCATTC ATCCCGGACA GTGCTGCCCG 
TCATGTTCAG ATGACTTTGT GGTGCAAAAG CCAGAGCTCA GCACCCCCTC CATTTGCCAT 
GCCCCTGGAG GAGAGTACTT CGTGGAAGGA GAGACATGGA ACATCGACTC GTGTACTCAG 
TGCACCTGCC ACAGCGGGCG GGTGCTGTGT GAGACGGAGG TGTGCCCACC GCTGCTCTGC 
GAGAACCCCT CGCGCACCCA GGACTCCTGC TGCCCGCAGT GCACAGATGA ACCTCTTCAG 
CCTTCCTCAT CCCATAATGA CAGTGTGCCT AGTTACTGCA AAAATGACGA AGGGGATATA 
TTCCTGGCAG CCGAGTCCTG GAAGCCTGAC GTCTGTACCA GCTGTGTATG CATGGATAGC 
GTAATTAGCT GTTACTCTGA ATCCTGCCCT TCAGTATCCT GTGAAAGACC TGTTTTGAGA 
AAAGGCCAGT GTTGTCCCTA TTGCATAGAA GACACGATTC CGAAGAAGGT GGTGTGCCAC 
TTCAGTGGGA AGGCCTACGC CGACGAGGAG CGCTGGGACA TTGACAGCTG CACACACTGC 
TACTGCCTGC AGGGCCAGAC CCTCTGCTCG ACCGTCAGCT GCCCCCCTCT GCCCTGTGTC 
GAGCCCATCA ACGTGGAAGG CAGTTGCTGC CCAATGTGTC CAGAAATGTA TGTCCCAGAA 
CCAACCAATA TACCCATTGA GAAAACAAAT CACCGTGGCG ACATCGACCT GGAGGTTCCC 
TTGTGGCCTA CACCAAGTGA AAATGACATC ATCCATCTTC CCAGAGACAT GGGTCACCTC 
CAGGTAGATT ACAGAGATAA CAACAGGCTG CATTCAGGCG AAGATTCTTC ACTGGACTCC 
ATTGCCTCGG TTGTGGTTCC CATCATCATC GGCCTCTCGA TTATAATAGC ATTCCTGTTC 
ATCAATCAGA AGAAACAGTG GATACCACTG CTTTGCTGGT ATCGAACACC AACTAAGCCT 
TCTTCCTTAA ATAATCAGCT GGTGTCTGTG GACTGCAAGA AAGGAAGCAG AGTCCCAGTG 
GACAGTTCAC AGAGAATGCT AAGAATTGCT GAACCAGATG CAAGATTCAG TGGCTTCTAC 
AGCATGCAAA AACAGAACCA CCTACAGGCA GATAATTTCT ACCAGACAGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_532931.2
CDS	10..3123
Translation	MYLVAGGRGLAGCGHLPVSLLGLLLLLARSGTRALVCLPCDESKCEEPRSCPGSIVQGVCGCCYMCARQRNESCGGAYGLHGACDRGLRCVIRPPLNGDSITEYEVGVCEDENWDDDQLLGFEPCNENLVSGCNIINGKCECDTIRTCNNPFEFPRKDMCLSALKRIEEEKPDCSKARCEVQFSPRCPEDSVLIEGYAPPGECCPLPSRCVCDPAGCLRKVCQPGYLNILVSKASGKPGECCDLYECKPVFSVDCSTVECPSVQQAVCPLDSYETQVRLTADGCCTLPTRCECLSGLCGFPVCEVGSTPRIVSRGDGTPGKCCDVFECVNETKPACVFNNVEYNDGDMFRMDNCRFCRCQGGVSICFTAQCGELNCERYYVPEGECCPVCEDPVYPFNNPAGCYANGQIRAHGDRWREDDCTFCQCINGEPHCVATACGQSCMNPVKVPGECCPVCEEPTYITIDPPACGELSNCTLTEKDCVYGFKLDHNGCRTCQCKNREELCSGLKRGCTLDCPFGFLTDAHNCEICQCRPRPKKCRPVICDKYCPFGYLKNKHGCNICRCKKCPELLCSKICPLGFQQDSHGCLICKCREVLASAGPPVLSGTCLSMDGHHHKNEESWHDGCRECYCHNGREMCALITCPVPACGNPTIHPGQCCPSCSDDFVVQKPELSTPSICHAPGGEYFVEGETWNIDSCTQCTCHSGRVLCETEVCPPLLCENPSRTQDSCCPQCTDEPLQPSSSHNDSVPSYCKNDEGDIFLAAESWKPDVCTSCVCMDSVISCYSESCPSVSCERPVLRKGQCCPYCIEDTIPKKVVCHFSGKAYADEERWDIDSCTHCYCLQGQTLCSTVSCPPLPCVEPINVEGSCCPMCPEMYVPEPTNIPIEKTNHRGDIDLEVPLWPTPSENDIIHLPRDMGHLQVDYRDNNRLHSGEDSSLDSIASVVVPIIIGLSIIIAFLFINQKKQWIPLLCWYRTPTKPSSLNNQLVSVDCKKGSRVPVDSSQRMLRIAEPDARFSGFYSMQKQNHLQADNFYQTV

Target ORF information:

RefSeq Version	XM_532931.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_532931.4

ORF Insert Sequence:

ATGTACTTGG TGGCGGGGGG CAGGGGGCTG GCCGGCTGCG GGCACCTCCC GGTCTCGCTG 
CTGGGGCTGC TGCTGCTGCT GGCGCGCTCC GGCACCCGGG CGCTGGTCTG CCTGCCCTGC 
GACGAGTCCA AGTGCGAGGA GCCCAGGAGC TGCCCCGGGA GCATCGTGCA GGGCGTCTGC 
GGCTGCTGCT ACATGTGCGC CCGCCAGAGG AACGAGAGCT GCGGCGGCGC CTACGGGCTC 
CACGGAGCCT GCGACCGGGG GCTGCGCTGT GTCATCCGCC CCCCGCTCAA TGGCGACTCC 
ATCACCGAGT ACGAAGTGGG CGTCTGCGAA GATGAGAACT GGGATGATGA CCAGCTGCTT 
GGTTTTGAAC CCTGCAATGA AAATCTTGTG TCTGGCTGCA ATATAATCAA TGGGAAATGC 
GAATGTGACA CCATTCGCAC CTGCAACAAT CCCTTTGAGT TTCCAAGGAA GGATATGTGC 
CTTTCAGCTT TGAAGAGGAT TGAAGAAGAG AAGCCAGATT GCTCCAAGGC CCGCTGTGAA 
GTCCAGTTCT CTCCACGTTG TCCTGAAGAT TCCGTTCTGA TCGAGGGCTA TGCTCCCCCC 
GGGGAGTGCT GCCCCTTACC CAGCCGCTGC GTGTGCGACC CTGCGGGCTG TCTGCGCAAA 
GTCTGCCAGC CGGGATACCT GAACATACTA GTGTCAAAGG CCTCAGGGAA GCCGGGAGAG 
TGTTGTGACC TCTATGAGTG CAAACCAGTT TTCAGCGTGG ACTGCAGCAC CGTGGAGTGC 
CCCTCCGTTC AGCAGGCCGT GTGCCCTCTG GACAGCTACG AGACTCAAGT CAGACTCACG 
GCGGACGGTT GCTGCACCCT GCCAACAAGA TGCGAGTGTC TCTCTGGCTT ATGTGGTTTC 
CCCGTGTGTG AGGTGGGATC CACTCCTCGC ATAGTCTCTC GTGGAGATGG GACACCTGGA 
AAGTGCTGTG ATGTCTTTGA ATGTGTTAAT GAAACAAAGC CAGCCTGTGT ATTCAACAAC 
GTGGAATATA ACGATGGGGA TATGTTTCGA ATGGACAACT GTCGATTCTG TCGATGCCAG 
GGGGGTGTTT CCATCTGCTT CACTGCCCAG TGTGGTGAGC TGAACTGCGA GAGGTATTAT 
GTGCCCGAAG GAGAGTGCTG CCCAGTGTGT GAAGATCCGG TGTATCCTTT TAATAATCCT 
GCTGGCTGCT ATGCCAATGG TCAGATCCGC GCGCACGGGG ACCGGTGGCG AGAAGACGAC 
TGCACTTTCT GCCAGTGTAT CAATGGAGAA CCCCACTGTG TGGCCACGGC CTGTGGGCAG 
AGCTGCATGA ACCCCGTCAA AGTGCCTGGC GAGTGTTGCC CTGTGTGTGA AGAGCCAACG 
TACATCACAA TTGATCCACC TGCATGTGGG GAGTTATCAA ACTGCACGCT GACAGAGAAG 
GACTGCGTTT ATGGTTTCAA ACTTGATCAC AATGGTTGTC GAACCTGTCA GTGCAAAAAC 
AGGGAGGAGC TGTGCTCGGG CCTCAAGCGA GGCTGTACTT TGGACTGCCC CTTCGGCTTC 
CTGACTGATG CCCACAACTG TGAGATCTGT CAGTGCCGCC CACGGCCCAA GAAATGCAGA 
CCTGTAATCT GTGACAAGTA TTGTCCATTT GGATATCTGA AGAATAAGCA TGGCTGTAAC 
ATCTGTCGCT GCAAGAAATG CCCAGAGCTC CTGTGCAGTA AAATTTGCCC CTTGGGTTTC 
CAGCAGGACA GTCATGGCTG TCTTATCTGC AAGTGCAGAG AGGTCCTTGC TTCAGCCGGG 
CCACCTGTCC TGTCAGGCAC ATGTCTGTCC ATGGATGGCC ATCATCATAA AAATGAGGAA 
AGCTGGCATG ATGGGTGCCG GGAATGCTAC TGTCACAATG GACGGGAAAT GTGTGCTCTG 
ATCACCTGCC CGGTGCCTGC CTGTGGCAAC CCCACCATTC ATCCCGGACA GTGCTGCCCG 
TCATGTTCAG ATGACTTTGT GGTGCAAAAG CCAGAGCTCA GCACCCCCTC CATTTGCCAT 
GCCCCTGGAG GAGAGTACTT CGTGGAAGGA GAGACATGGA ACATCGACTC GTGTACTCAG 
TGCACCTGCC ACAGCGGGCG GGTGCTGTGT GAGACGGAGG TGTGCCCACC GCTGCTCTGC 
GAGAACCCCT CGCGCACCCA GGACTCCTGC TGCCCGCAGT GCACAGATGA ACCTCTTCAG 
CCTTCCTCAT CCCATAATGA CAGTGTGCCT AGTTACTGCA AAAATGACGA AGGGGATATA 
TTCCTGGCAG CCGAGTCCTG GAAGCCTGAC GTCTGTACCA GCTGTGTATG CATGGATAGC 
GTAATTAGCT GTTACTCTGA ATCCTGCCCT TCAGTATCCT GTGAAAGACC TGTTTTGAGA 
AAAGGCCAGT GTTGTCCCTA TTGCATAGAA GACACGATTC CGAAGAAGGT GGTGTGCCAC 
TTCAGTGGGA AGGCCTACGC CGACGAGGAG CGCTGGGACA TTGACAGCTG CACACACTGC 
TACTGCCTGC AGGGCCAGAC CCTCTGCTCG ACCGTCAGCT GCCCCCCTCT GCCCTGTGTC 
GAGCCCATCA ACGTGGAAGG CAGTTGCTGC CCAATGTGTC CAGAAATGTA TGTCCCAGAA 
CCAACCAATA TACCCATTGA GAAAACAAAT CACCGTGGCG ACATCGACCT GGAGGTTCCC 
TTGTGGCCTA CACCAAGTGA AAATGACATC ATCCATCTTC CCAGAGACAT GGGTCACCTC 
CAGGTAGATT ACAGAGATAA CAACAGGCTG CATTCAGGCG AAGATTCTTC ACTGGACTCC 
ATTGCCTCGG TTGTGGTTCC CATCATCATC GGCCTCTCGA TTATAATAGC ATTCCTGTTC 
ATCAATCAGA AGAAACAGTG GATACCACTG CTTTGCTGGT ATCGAACACC AACTAAGCCT 
TCTTCCTTAA ATAATCAGCT GGTGTCTGTG GACTGCAAGA AAGGAAGCAG AGTCCCAGTG 
GACAGTTCAC AGAGAATGCT AAGAATTGCT GAACCAGATG CAAGATTCAG TGGCTTCTAC 
AGCATGCAAA AACAGAACCA CCTACAGGCA GATAATTTCT ACCAGACAGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRIM1 ( XM_005630329.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005630386.1 Canis lupus familiaris cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X2, mRNA.

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CloneID	OCe18879
Clone ID Related Accession (Same CDS sequence)	XM_005630329.2
Accession Version	XM_005630329.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3111bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	cysteine-rich motor neuron 1 protein isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005630329.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTTGG TGGCGGGGGG CAGGGGGCTG GCCGGCTGCG GGCACCTCCC GGTCTCGCTG 
CTGGGGCTGC TGCTGCTGCT GGCGCGCTCC GGCACCCGGG CGCTGGTCTG CCTGCCCTGC 
GACGAGTCCA AGTGCGAGGA GCCCAGGAGC TGCCCCGGGA GCATCGTGCA GGGCGTCTGC 
GGCTGCTGCT ACATGTGCGC CCGCCAGAGG AACGAGAGCT GCGGCGGCGC CTACGGGCTC 
CACGGAGCCT GCGACCGGGG GCTGCGCTGT GTCATCCGCC CCCCGCTCAA TGGCGACTCC 
ATCACCGAGT ACGAAGTGGG CGTCTGCGAA GATGAGAACT GGGATGATGA CCAGCTGCTT 
GGTTTTGAAC CCTGCAATGA AAATCTTGTG TCTGGCTGCA ATATAATCAA TGGGAAATGC 
GAATGTGACA CCATTCGCAC CTGCAACAAT CCCTTTGAGT TTCCAAGGAA GGATATGTGC 
CTTTCAGCTT TGAAGAGGAT TGAAGAAGAG AAGCCAGATT GCTCCAAGGC CCGCTGTGAA 
GTCCAGTTCT CTCCACGTTG TCCTGAAGAT TCCGTTCTGA TCGAGGGCTA TGCTCCCCCC 
GGGGAGTGCT GCCCCTTACC CAGCCGCTGC GTGTGCGACC CTGCGGGCTG TCTGCGCAAA 
GTCTGCCAGC CGGGATACCT GAACATACTA GTGTCAAAGG CCTCAGGGAA GCCGGGAGAG 
TGTTGTGACC TCTATGAGTG CAAACCAGTT TTCAGCGTGG ACTGCAGCAC CGTGGAGTGC 
CCCTCCGTTC AGCAGGCCGT GTGCCCTCTG GACAGCTACG AGACTCAAGT CAGACTCACG 
GCGGACGGTT GCTGCACCCT GCCAACAAGA TGCGAGTGTC TCTCTGGCTT ATGTGGTTTC 
CCCGTGTGTG AGGTGGGATC CACTCCTCGC ATAGTCTCTC GTGGAGATGG GACACCTGGA 
AAGTGCTGTG ATGTCTTTGA ATGTGTTAAT GAAACAAAGC CAGCCTGTGT ATTCAACAAC 
GTGGAATATA ACGATGGGGA TATGTTTCGA ATGGACAACT GTCGATTCTG TCGATGCCAG 
GGGGGTGTTT CCATCTGCTT CACTGCCCAG TGTGGTGAGC TGAACTGCGA GAGGTATTAT 
GTGCCCGAAG GAGAGTGCTG CCCAGTGTGT GAAGATCCGG TGTATCCTTT TAATAATCCT 
GCTGGCTGCT ATGCCAATGG TCAGATCCGC GCGCACGGGG ACCGGTGGCG AGAAGACGAC 
TGCACTTTCT GCCAGTGTAT CAATGGAGAA CCCCACTGTG TGGCCACGGC CTGTGGGCAG 
AGCTGCATGA ACCCCGTCAA AGTGCCTGGC GAGTGTTGCC CTGTGTGTGA AGAGCCAACG 
TACATCACAA TTGATCCACC TGCATGTGGG GAGTTATCAA ACTGCACGCT GACAGAGAAG 
GACTGCGTTT ATGGTTTCAA ACTTGATCAC AATGGTTGTC GAACCTGTCA GTGCAAAAAC 
AGGGAGGAGC TGTGCTCGGG CCTCAAGCGA GGCTGTACTT TGGACTGCCC CTTCGGCTTC 
CTGACTGATG CCCACAACTG TGAGATCTGT CAGTGCCGCC CACGGCCCAA GAAATGCAGA 
CCTGTAATCT GTGACAAGTA TTGTCCATTT GGATATCTGA AGAATAAGCA TGGCTGTAAC 
ATCTGTCGCT GCAAGAAATG CCCAGAGCTC CTGTGCAGTA AAATTTGCCC CTTGGGTTTC 
CAGCAGGACA GTCATGGCTG TCTTATCTGC AAGTGCAGAG AGGTCCTTGC TTCAGCCGGG 
CCACCTGTCC TGTCAGGCAC ATGTCTGTCC ATGGATGGCC ATCATCATAA AAATGAGGAA 
AGCTGGCATG ATGGGTGCCG GGAATGCTAC TGTCACAATG GACGGGAAAT GTGTGCTCTG 
ATCACCTGCC CGGTGCCTGC CTGTGGCAAC CCCACCATTC ATCCCGGACA GTGCTGCCCG 
TCATGTTCAG ATGACTTTGT GGTGCAAAAG CCAGAGCTCA GCACCCCCTC CATTTGCCAT 
GCCCCTGGAG GAGAGTACTT CGTGGAAGGA GAGACATGGA ACATCGACTC GTGTACTCAG 
TGCACCTGCC ACAGCGGGCG GGTGCTGTGT GAGACGGAGG TGTGCCCACC GCTGCTCTGC 
GAGAACCCCT CGCGCACCCA GGACTCCTGC TGCCCGCAGT GCACAGATGA ACCTCTTCAG 
CCTTCCTCAT CCCATAATGA CAGTGTGCCT AGTTACTGCA AAAATGACGA AGGGGATATA 
TTCCTGGCAG CCGAGTCCTG GAAGCCTGAC GTCTGTACCA GCTGTGTATG CATGGATAGC 
GTAATTAGCT GTTACTCTGA ATCCTGCCCT TCAGTATCCT GTGAAAGACC TGTTTTGAGA 
AAAGGCCAGT GTTGTCCCTA TTGCATAGAC ACGATTCCGA AGAAGGTGGT GTGCCACTTC 
AGTGGGAAGG CCTACGCCGA CGAGGAGCGC TGGGACATTG ACAGCTGCAC ACACTGCTAC 
TGCCTGCAGG GCCAGACCCT CTGCTCGACC GTCAGCTGCC CCCCTCTGCC CTGTGTCGAG 
CCCATCAACG TGGAAGGCAG TTGCTGCCCA ATGTGTCCAG AAATGTATGT CCCAGAACCA 
ACCAATATAC CCATTGAGAA AACAAATCAC CGTGGCGACA TCGACCTGGA GGTTCCCTTG 
TGGCCTACAC CAAGTGAAAA TGACATCATC CATCTTCCCA GAGACATGGG TCACCTCCAG 
GTAGATTACA GAGATAACAA CAGGCTGCAT TCAGGCGAAG ATTCTTCACT GGACTCCATT 
GCCTCGGTTG TGGTTCCCAT CATCATCGGC CTCTCGATTA TAATAGCATT CCTGTTCATC 
AATCAGAAGA AACAGTGGAT ACCACTGCTT TGCTGGTATC GAACACCAAC TAAGCCTTCT 
TCCTTAAATA ATCAGCTGGT GTCTGTGGAC TGCAAGAAAG GAAGCAGAGT CCCAGTGGAC 
AGTTCACAGA GAATGCTAAG AATTGCTGAA CCAGATGCAA GATTCAGTGG CTTCTACAGC 
ATGCAAAAAC AGAACCACCT ACAGGCAGAT AATTTCTACC AGACAGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005630386.1
CDS	10..3120
Translation	MYLVAGGRGLAGCGHLPVSLLGLLLLLARSGTRALVCLPCDESKCEEPRSCPGSIVQGVCGCCYMCARQRNESCGGAYGLHGACDRGLRCVIRPPLNGDSITEYEVGVCEDENWDDDQLLGFEPCNENLVSGCNIINGKCECDTIRTCNNPFEFPRKDMCLSALKRIEEEKPDCSKARCEVQFSPRCPEDSVLIEGYAPPGECCPLPSRCVCDPAGCLRKVCQPGYLNILVSKASGKPGECCDLYECKPVFSVDCSTVECPSVQQAVCPLDSYETQVRLTADGCCTLPTRCECLSGLCGFPVCEVGSTPRIVSRGDGTPGKCCDVFECVNETKPACVFNNVEYNDGDMFRMDNCRFCRCQGGVSICFTAQCGELNCERYYVPEGECCPVCEDPVYPFNNPAGCYANGQIRAHGDRWREDDCTFCQCINGEPHCVATACGQSCMNPVKVPGECCPVCEEPTYITIDPPACGELSNCTLTEKDCVYGFKLDHNGCRTCQCKNREELCSGLKRGCTLDCPFGFLTDAHNCEICQCRPRPKKCRPVICDKYCPFGYLKNKHGCNICRCKKCPELLCSKICPLGFQQDSHGCLICKCREVLASAGPPVLSGTCLSMDGHHHKNEESWHDGCRECYCHNGREMCALITCPVPACGNPTIHPGQCCPSCSDDFVVQKPELSTPSICHAPGGEYFVEGETWNIDSCTQCTCHSGRVLCETEVCPPLLCENPSRTQDSCCPQCTDEPLQPSSSHNDSVPSYCKNDEGDIFLAAESWKPDVCTSCVCMDSVISCYSESCPSVSCERPVLRKGQCCPYCIDTIPKKVVCHFSGKAYADEERWDIDSCTHCYCLQGQTLCSTVSCPPLPCVEPINVEGSCCPMCPEMYVPEPTNIPIEKTNHRGDIDLEVPLWPTPSENDIIHLPRDMGHLQVDYRDNNRLHSGEDSSLDSIASVVVPIIIGLSIIIAFLFINQKKQWIPLLCWYRTPTKPSSLNNQLVSVDCKKGSRVPVDSSQRMLRIAEPDARFSGFYSMQKQNHLQADNFYQTV

Target ORF information:

RefSeq Version	XM_005630329.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005630329.2

ORF Insert Sequence:

ATGTACTTGG TGGCGGGGGG CAGGGGGCTG GCCGGCTGCG GGCACCTCCC GGTCTCGCTG 
CTGGGGCTGC TGCTGCTGCT GGCGCGCTCC GGCACCCGGG CGCTGGTCTG CCTGCCCTGC 
GACGAGTCCA AGTGCGAGGA GCCCAGGAGC TGCCCCGGGA GCATCGTGCA GGGCGTCTGC 
GGCTGCTGCT ACATGTGCGC CCGCCAGAGG AACGAGAGCT GCGGCGGCGC CTACGGGCTC 
CACGGAGCCT GCGACCGGGG GCTGCGCTGT GTCATCCGCC CCCCGCTCAA TGGCGACTCC 
ATCACCGAGT ACGAAGTGGG CGTCTGCGAA GATGAGAACT GGGATGATGA CCAGCTGCTT 
GGTTTTGAAC CCTGCAATGA AAATCTTGTG TCTGGCTGCA ATATAATCAA TGGGAAATGC 
GAATGTGACA CCATTCGCAC CTGCAACAAT CCCTTTGAGT TTCCAAGGAA GGATATGTGC 
CTTTCAGCTT TGAAGAGGAT TGAAGAAGAG AAGCCAGATT GCTCCAAGGC CCGCTGTGAA 
GTCCAGTTCT CTCCACGTTG TCCTGAAGAT TCCGTTCTGA TCGAGGGCTA TGCTCCCCCC 
GGGGAGTGCT GCCCCTTACC CAGCCGCTGC GTGTGCGACC CTGCGGGCTG TCTGCGCAAA 
GTCTGCCAGC CGGGATACCT GAACATACTA GTGTCAAAGG CCTCAGGGAA GCCGGGAGAG 
TGTTGTGACC TCTATGAGTG CAAACCAGTT TTCAGCGTGG ACTGCAGCAC CGTGGAGTGC 
CCCTCCGTTC AGCAGGCCGT GTGCCCTCTG GACAGCTACG AGACTCAAGT CAGACTCACG 
GCGGACGGTT GCTGCACCCT GCCAACAAGA TGCGAGTGTC TCTCTGGCTT ATGTGGTTTC 
CCCGTGTGTG AGGTGGGATC CACTCCTCGC ATAGTCTCTC GTGGAGATGG GACACCTGGA 
AAGTGCTGTG ATGTCTTTGA ATGTGTTAAT GAAACAAAGC CAGCCTGTGT ATTCAACAAC 
GTGGAATATA ACGATGGGGA TATGTTTCGA ATGGACAACT GTCGATTCTG TCGATGCCAG 
GGGGGTGTTT CCATCTGCTT CACTGCCCAG TGTGGTGAGC TGAACTGCGA GAGGTATTAT 
GTGCCCGAAG GAGAGTGCTG CCCAGTGTGT GAAGATCCGG TGTATCCTTT TAATAATCCT 
GCTGGCTGCT ATGCCAATGG TCAGATCCGC GCGCACGGGG ACCGGTGGCG AGAAGACGAC 
TGCACTTTCT GCCAGTGTAT CAATGGAGAA CCCCACTGTG TGGCCACGGC CTGTGGGCAG 
AGCTGCATGA ACCCCGTCAA AGTGCCTGGC GAGTGTTGCC CTGTGTGTGA AGAGCCAACG 
TACATCACAA TTGATCCACC TGCATGTGGG GAGTTATCAA ACTGCACGCT GACAGAGAAG 
GACTGCGTTT ATGGTTTCAA ACTTGATCAC AATGGTTGTC GAACCTGTCA GTGCAAAAAC 
AGGGAGGAGC TGTGCTCGGG CCTCAAGCGA GGCTGTACTT TGGACTGCCC CTTCGGCTTC 
CTGACTGATG CCCACAACTG TGAGATCTGT CAGTGCCGCC CACGGCCCAA GAAATGCAGA 
CCTGTAATCT GTGACAAGTA TTGTCCATTT GGATATCTGA AGAATAAGCA TGGCTGTAAC 
ATCTGTCGCT GCAAGAAATG CCCAGAGCTC CTGTGCAGTA AAATTTGCCC CTTGGGTTTC 
CAGCAGGACA GTCATGGCTG TCTTATCTGC AAGTGCAGAG AGGTCCTTGC TTCAGCCGGG 
CCACCTGTCC TGTCAGGCAC ATGTCTGTCC ATGGATGGCC ATCATCATAA AAATGAGGAA 
AGCTGGCATG ATGGGTGCCG GGAATGCTAC TGTCACAATG GACGGGAAAT GTGTGCTCTG 
ATCACCTGCC CGGTGCCTGC CTGTGGCAAC CCCACCATTC ATCCCGGACA GTGCTGCCCG 
TCATGTTCAG ATGACTTTGT GGTGCAAAAG CCAGAGCTCA GCACCCCCTC CATTTGCCAT 
GCCCCTGGAG GAGAGTACTT CGTGGAAGGA GAGACATGGA ACATCGACTC GTGTACTCAG 
TGCACCTGCC ACAGCGGGCG GGTGCTGTGT GAGACGGAGG TGTGCCCACC GCTGCTCTGC 
GAGAACCCCT CGCGCACCCA GGACTCCTGC TGCCCGCAGT GCACAGATGA ACCTCTTCAG 
CCTTCCTCAT CCCATAATGA CAGTGTGCCT AGTTACTGCA AAAATGACGA AGGGGATATA 
TTCCTGGCAG CCGAGTCCTG GAAGCCTGAC GTCTGTACCA GCTGTGTATG CATGGATAGC 
GTAATTAGCT GTTACTCTGA ATCCTGCCCT TCAGTATCCT GTGAAAGACC TGTTTTGAGA 
AAAGGCCAGT GTTGTCCCTA TTGCATAGAC ACGATTCCGA AGAAGGTGGT GTGCCACTTC 
AGTGGGAAGG CCTACGCCGA CGAGGAGCGC TGGGACATTG ACAGCTGCAC ACACTGCTAC 
TGCCTGCAGG GCCAGACCCT CTGCTCGACC GTCAGCTGCC CCCCTCTGCC CTGTGTCGAG 
CCCATCAACG TGGAAGGCAG TTGCTGCCCA ATGTGTCCAG AAATGTATGT CCCAGAACCA 
ACCAATATAC CCATTGAGAA AACAAATCAC CGTGGCGACA TCGACCTGGA GGTTCCCTTG 
TGGCCTACAC CAAGTGAAAA TGACATCATC CATCTTCCCA GAGACATGGG TCACCTCCAG 
GTAGATTACA GAGATAACAA CAGGCTGCAT TCAGGCGAAG ATTCTTCACT GGACTCCATT 
GCCTCGGTTG TGGTTCCCAT CATCATCGGC CTCTCGATTA TAATAGCATT CCTGTTCATC 
AATCAGAAGA AACAGTGGAT ACCACTGCTT TGCTGGTATC GAACACCAAC TAAGCCTTCT 
TCCTTAAATA ATCAGCTGGT GTCTGTGGAC TGCAAGAAAG GAAGCAGAGT CCCAGTGGAC 
AGTTCACAGA GAATGCTAAG AATTGCTGAA CCAGATGCAA GATTCAGTGG CTTCTACAGC 
ATGCAAAAAC AGAACCACCT ACAGGCAGAT AATTTCTACC AGACAGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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CRIM1 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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