HACE1 cDNA ORF clone, Canis lupus familiaris(dog)

The following HACE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HACE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe15637 XM_849139.5
Latest version!
Canis lupus familiaris HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe15638 XM_005627667.3
Latest version!
Canis lupus familiaris HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe292658 XM_022426399.1
Latest version!
Canis lupus familiaris HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe292659 XM_022426401.1
Latest version!
Canis lupus familiaris HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OCe292659 XM_022426402.1
Latest version!
Canis lupus familiaris HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OCe292660 XM_022426403.1
Latest version!
Canis lupus familiaris HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
View Old Accession Versions >>
OCe15637 XM_849139.4 Canis lupus familiaris HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe15638 XM_005627667.2 Canis lupus familiaris HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe15637
    Clone ID Related Accession (Same CDS sequence) XM_849139.4 , XM_849139.5
    Accession Version XM_849139.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2730bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase HACE1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_849139.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAGAGAG CGATGGAGCA GCTCAACCGC CTCACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CGGACGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT
    GATCAACACA GGTCTGTTTC TGAGCTACTA TCAAATTCAA AGTTTGATGT CAATTATGCT
    TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC
    TTGGTCCTGC TATTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC AGGATGTACA
    CCCCTTCATT TGGCAGCTAG AAATGGGCAG AAGAAATGCA TGAGTAAATT ATTAGAATAC
    AGTGCTGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAATACACTG GCTCGCTGTG
    AATGGGCGGA CAGAACTACT CCATGACCTT GTGCAGCACG TCAGTGACGT CGACGTTGAG
    GATGCGATGG GGCAGACAGC ACTGCATGTC GCCTGCCAGA ATGGTCACAA GACGACAGTG
    CAGTGCTTGC TAGACAGTGG TGCTGATATT AACAGGCCAA ATGTATCTGG AGCCACTCCA
    TTGTACTTTG CTTGCAGTCA TGGTCAGAGA GACACAGCAC AAATTCTTCT GTTACGAGGA
    GCTAAATATC TGTCAGATAA AAATGGAGTA ACGCCTCTGG ATTTATGTGT ACAGGGTGGC
    TATGGAGAGA CTTGTGAAGT ATTAATTCAG TATCACCCTA GGCTTTTCCA GACAATTATT
    CAAATGACAC AGAATGAAGA TCTCCGAGAA AACATGTTAC GGCAAGTTCT GGAACATTTG
    TCTCAGCAAA GTGAAAGCCA GTACCTAAAG ATTCTAACAA GCCTTGCTGA AGTTGCCACA
    ACAAATGGGC ATAAACTGCT TAGTCTGTCT AGTAATTATG ATGCTCAGAT GAAGAGCCTC
    TTAAGGATTG TGAGGATATT TTGTCATGTC TTTCGAATTG GTCCATCCTC TCCCAGTAAT
    GGAATTGATT TGGGCTACAA CGGGAATAAA ACTCCAAGAA GCCAAGTGTT CAAGCCTCTT
    GAATTGCTTT GGCACTCATT AGATGAATGG CTAGTTTTAA TAGCTACAGA ATTGATGAAA
    AACAAAAAGG ACTCAACGGA TATCACTTCT ATTTTACTGA AACAAAAAGG CCAAGATCAA
    GATGGTACTT CCATTCCTCC ATTTGAACCT CCAGGACCTG GGAGCTATGA AAATCTATCC
    AGTGGCACAG GAGAATCTAA ACCAGATGCT CTTGGAGGGA AACAGGAAGC CAGTGCAGAC
    TGTCAGGATG TTATTTCTAT GACAGCTAAC CGGCTAAGTG CTGTCATTCA AGCTTTTTAC
    ATGTGCTGTT CTTGTCAGAT GCCTCCAGGA ATGACCTCAC CCCGTTTCAT TGAATTTGTC
    TGCAAACATG ATGAAGTTTT AAAATGCTTT GTTAATAGAA ATCCCAAAAT TATATTTGAT
    CACTTTCATT TCCTCCTTGA ATGTCCTGAG TTGATGTCAA GATTCATGCA TATCATAAAA
    GCACAGCCAT TTAAAGATCG CTGTGAGTGG TTCTATGAAC ATTTGCACTC AGGACAACCA
    GATTCTGACA TGGTGCACAG GCCAGTGAAT GAAAATGATA TCCTGCTTGT TCACAGAGAT
    TCTATTTTTA GGAGTAGCTG TGAAGTTGTG TCAAAAGCAA ACTGTGCAAA GCTAAAGCAA
    GGAATTGCTG TACGGTTCCA TGGAGAAGAA GGCATGGGCC AAGGTGTTGT GCGTGAGTGG
    TTTGATATTC TGTCTAATGA GATAGTGAAT CCTGATTATG CATTGTTCAC CCAGTCAGCT
    GATGGAACAA CTTTTCAGCC TAATAGTAAT TCCTATGTAA ATCCTGATCA CTTGAACTAT
    TTCCGGTTTG CTGGACAGAT CTTGGGATTA GCTTTGAACC ACAGGCAGCT GGTCAACATT
    TACTTCACAA GATCGTTCTA TAAGCACATT CTTGGTATCC CTGTAAATTA CCAAGATGTG
    GCATCCATTG ATCCAGAATA TGCCAAAAAT TTGCAGTGGA TTTTAGATAA TGACATTAGT
    GATCTGGGTC TAGAACTAAC CTTTTCTGTT GAAACTGATG TGTTTGGAGC AATGGAAGAG
    GTGCCTTTGA AACCTGGGGG TGGAAGTATT CTTGTGACAC AAAATAACAA AGCGGAGTAT
    GTCCAGCTTG TTACTGAACT TCGAATGACA AGAGCCATTC AACCTCAGAT CAATGCTTTT
    TTACAGGGCT TTCATATGTT CATTCCACCT TCCCTCATAC AACTTTTTGA TGAATATGAA
    TTGGAGCTAC TGCTTTCTGG CATGCCAGAA ATTGATGTGA GCGATTGGAT AAGAAATACA
    GAATACACAA GTGGCTATGA AAGAGAAGAT CCAGTTATTC AGTGGTTCTG GGAAGTTGTG
    GAAGGCATTA CTCCAGAGGA GAGAGTTCTT CTCTTGCAGT TTGTTACTGG CAGTTCCAGG
    GTCCCACATG GTGGATTTGC TAACATCATG GGTGGAAGTG GATTGCAAAA CTTTACAATT
    GCTGCTGTGC CATATACTCC AAATCTCCTA CCAACTTCAA GCACATGCAT CAACATGCTC
    AAGTTACCTG AATACCCAAG TAAAGAAATA CTCAAGGACA GACTTCTTGT GGCGTTACAT
    TGTGGCAGCT ATGGTTACAC AATGGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_854232.1
    CDS190..2919
    Translation

    Target ORF information:

    RefSeq Version XM_849139.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_849139.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAGAGAG CGATGGAGCA GCTCAACCGC CTCACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CGGACGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT
    GATCAACACA GGTCTGTTTC TGAGCTACTA TCAAATTCAA AGTTTGATGT CAATTATGCT
    TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC
    TTGGTCCTGC TATTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC AGGATGTACA
    CCCCTTCATT TGGCAGCTAG AAATGGGCAG AAGAAATGCA TGAGTAAATT ATTAGAATAC
    AGTGCTGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAATACACTG GCTCGCTGTG
    AATGGGCGGA CAGAACTACT CCATGACCTT GTGCAGCACG TCAGTGACGT CGACGTTGAG
    GATGCGATGG GGCAGACAGC ACTGCATGTC GCCTGCCAGA ATGGTCACAA GACGACAGTG
    CAGTGCTTGC TAGACAGTGG TGCTGATATT AACAGGCCAA ATGTATCTGG AGCCACTCCA
    TTGTACTTTG CTTGCAGTCA TGGTCAGAGA GACACAGCAC AAATTCTTCT GTTACGAGGA
    GCTAAATATC TGTCAGATAA AAATGGAGTA ACGCCTCTGG ATTTATGTGT ACAGGGTGGC
    TATGGAGAGA CTTGTGAAGT ATTAATTCAG TATCACCCTA GGCTTTTCCA GACAATTATT
    CAAATGACAC AGAATGAAGA TCTCCGAGAA AACATGTTAC GGCAAGTTCT GGAACATTTG
    TCTCAGCAAA GTGAAAGCCA GTACCTAAAG ATTCTAACAA GCCTTGCTGA AGTTGCCACA
    ACAAATGGGC ATAAACTGCT TAGTCTGTCT AGTAATTATG ATGCTCAGAT GAAGAGCCTC
    TTAAGGATTG TGAGGATATT TTGTCATGTC TTTCGAATTG GTCCATCCTC TCCCAGTAAT
    GGAATTGATT TGGGCTACAA CGGGAATAAA ACTCCAAGAA GCCAAGTGTT CAAGCCTCTT
    GAATTGCTTT GGCACTCATT AGATGAATGG CTAGTTTTAA TAGCTACAGA ATTGATGAAA
    AACAAAAAGG ACTCAACGGA TATCACTTCT ATTTTACTGA AACAAAAAGG CCAAGATCAA
    GATGGTACTT CCATTCCTCC ATTTGAACCT CCAGGACCTG GGAGCTATGA AAATCTATCC
    AGTGGCACAG GAGAATCTAA ACCAGATGCT CTTGGAGGGA AACAGGAAGC CAGTGCAGAC
    TGTCAGGATG TTATTTCTAT GACAGCTAAC CGGCTAAGTG CTGTCATTCA AGCTTTTTAC
    ATGTGCTGTT CTTGTCAGAT GCCTCCAGGA ATGACCTCAC CCCGTTTCAT TGAATTTGTC
    TGCAAACATG ATGAAGTTTT AAAATGCTTT GTTAATAGAA ATCCCAAAAT TATATTTGAT
    CACTTTCATT TCCTCCTTGA ATGTCCTGAG TTGATGTCAA GATTCATGCA TATCATAAAA
    GCACAGCCAT TTAAAGATCG CTGTGAGTGG TTCTATGAAC ATTTGCACTC AGGACAACCA
    GATTCTGACA TGGTGCACAG GCCAGTGAAT GAAAATGATA TCCTGCTTGT TCACAGAGAT
    TCTATTTTTA GGAGTAGCTG TGAAGTTGTG TCAAAAGCAA ACTGTGCAAA GCTAAAGCAA
    GGAATTGCTG TACGGTTCCA TGGAGAAGAA GGCATGGGCC AAGGTGTTGT GCGTGAGTGG
    TTTGATATTC TGTCTAATGA GATAGTGAAT CCTGATTATG CATTGTTCAC CCAGTCAGCT
    GATGGAACAA CTTTTCAGCC TAATAGTAAT TCCTATGTAA ATCCTGATCA CTTGAACTAT
    TTCCGGTTTG CTGGACAGAT CTTGGGATTA GCTTTGAACC ACAGGCAGCT GGTCAACATT
    TACTTCACAA GATCGTTCTA TAAGCACATT CTTGGTATCC CTGTAAATTA CCAAGATGTG
    GCATCCATTG ATCCAGAATA TGCCAAAAAT TTGCAGTGGA TTTTAGATAA TGACATTAGT
    GATCTGGGTC TAGAACTAAC CTTTTCTGTT GAAACTGATG TGTTTGGAGC AATGGAAGAG
    GTGCCTTTGA AACCTGGGGG TGGAAGTATT CTTGTGACAC AAAATAACAA AGCGGAGTAT
    GTCCAGCTTG TTACTGAACT TCGAATGACA AGAGCCATTC AACCTCAGAT CAATGCTTTT
    TTACAGGGCT TTCATATGTT CATTCCACCT TCCCTCATAC AACTTTTTGA TGAATATGAA
    TTGGAGCTAC TGCTTTCTGG CATGCCAGAA ATTGATGTGA GCGATTGGAT AAGAAATACA
    GAATACACAA GTGGCTATGA AAGAGAAGAT CCAGTTATTC AGTGGTTCTG GGAAGTTGTG
    GAAGGCATTA CTCCAGAGGA GAGAGTTCTT CTCTTGCAGT TTGTTACTGG CAGTTCCAGG
    GTCCCACATG GTGGATTTGC TAACATCATG GGTGGAAGTG GATTGCAAAA CTTTACAATT
    GCTGCTGTGC CATATACTCC AAATCTCCTA CCAACTTCAA GCACATGCAT CAACATGCTC
    AAGTTACCTG AATACCCAAG TAAAGAAATA CTCAAGGACA GACTTCTTGT GGCGTTACAT
    TGTGGCAGCT ATGGTTACAC AATGGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe15637
    Clone ID Related Accession (Same CDS sequence) XM_849139.4 , XM_849139.5
    Accession Version XM_849139.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2730bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase HACE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_849139.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAGAGAG CGATGGAGCA GCTCAACCGC CTCACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CGGACGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT
    GATCAACACA GGTCTGTTTC TGAGCTACTA TCAAATTCAA AGTTTGATGT CAATTATGCT
    TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC
    TTGGTCCTGC TATTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC AGGATGTACA
    CCCCTTCATT TGGCAGCTAG AAATGGGCAG AAGAAATGCA TGAGTAAATT ATTAGAATAC
    AGTGCTGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAATACACTG GCTCGCTGTG
    AATGGGCGGA CAGAACTACT CCATGACCTT GTGCAGCACG TCAGTGACGT CGACGTTGAG
    GATGCGATGG GGCAGACAGC ACTGCATGTC GCCTGCCAGA ATGGTCACAA GACGACAGTG
    CAGTGCTTGC TAGACAGTGG TGCTGATATT AACAGGCCAA ATGTATCTGG AGCCACTCCA
    TTGTACTTTG CTTGCAGTCA TGGTCAGAGA GACACAGCAC AAATTCTTCT GTTACGAGGA
    GCTAAATATC TGTCAGATAA AAATGGAGTA ACGCCTCTGG ATTTATGTGT ACAGGGTGGC
    TATGGAGAGA CTTGTGAAGT ATTAATTCAG TATCACCCTA GGCTTTTCCA GACAATTATT
    CAAATGACAC AGAATGAAGA TCTCCGAGAA AACATGTTAC GGCAAGTTCT GGAACATTTG
    TCTCAGCAAA GTGAAAGCCA GTACCTAAAG ATTCTAACAA GCCTTGCTGA AGTTGCCACA
    ACAAATGGGC ATAAACTGCT TAGTCTGTCT AGTAATTATG ATGCTCAGAT GAAGAGCCTC
    TTAAGGATTG TGAGGATATT TTGTCATGTC TTTCGAATTG GTCCATCCTC TCCCAGTAAT
    GGAATTGATT TGGGCTACAA CGGGAATAAA ACTCCAAGAA GCCAAGTGTT CAAGCCTCTT
    GAATTGCTTT GGCACTCATT AGATGAATGG CTAGTTTTAA TAGCTACAGA ATTGATGAAA
    AACAAAAAGG ACTCAACGGA TATCACTTCT ATTTTACTGA AACAAAAAGG CCAAGATCAA
    GATGGTACTT CCATTCCTCC ATTTGAACCT CCAGGACCTG GGAGCTATGA AAATCTATCC
    AGTGGCACAG GAGAATCTAA ACCAGATGCT CTTGGAGGGA AACAGGAAGC CAGTGCAGAC
    TGTCAGGATG TTATTTCTAT GACAGCTAAC CGGCTAAGTG CTGTCATTCA AGCTTTTTAC
    ATGTGCTGTT CTTGTCAGAT GCCTCCAGGA ATGACCTCAC CCCGTTTCAT TGAATTTGTC
    TGCAAACATG ATGAAGTTTT AAAATGCTTT GTTAATAGAA ATCCCAAAAT TATATTTGAT
    CACTTTCATT TCCTCCTTGA ATGTCCTGAG TTGATGTCAA GATTCATGCA TATCATAAAA
    GCACAGCCAT TTAAAGATCG CTGTGAGTGG TTCTATGAAC ATTTGCACTC AGGACAACCA
    GATTCTGACA TGGTGCACAG GCCAGTGAAT GAAAATGATA TCCTGCTTGT TCACAGAGAT
    TCTATTTTTA GGAGTAGCTG TGAAGTTGTG TCAAAAGCAA ACTGTGCAAA GCTAAAGCAA
    GGAATTGCTG TACGGTTCCA TGGAGAAGAA GGCATGGGCC AAGGTGTTGT GCGTGAGTGG
    TTTGATATTC TGTCTAATGA GATAGTGAAT CCTGATTATG CATTGTTCAC CCAGTCAGCT
    GATGGAACAA CTTTTCAGCC TAATAGTAAT TCCTATGTAA ATCCTGATCA CTTGAACTAT
    TTCCGGTTTG CTGGACAGAT CTTGGGATTA GCTTTGAACC ACAGGCAGCT GGTCAACATT
    TACTTCACAA GATCGTTCTA TAAGCACATT CTTGGTATCC CTGTAAATTA CCAAGATGTG
    GCATCCATTG ATCCAGAATA TGCCAAAAAT TTGCAGTGGA TTTTAGATAA TGACATTAGT
    GATCTGGGTC TAGAACTAAC CTTTTCTGTT GAAACTGATG TGTTTGGAGC AATGGAAGAG
    GTGCCTTTGA AACCTGGGGG TGGAAGTATT CTTGTGACAC AAAATAACAA AGCGGAGTAT
    GTCCAGCTTG TTACTGAACT TCGAATGACA AGAGCCATTC AACCTCAGAT CAATGCTTTT
    TTACAGGGCT TTCATATGTT CATTCCACCT TCCCTCATAC AACTTTTTGA TGAATATGAA
    TTGGAGCTAC TGCTTTCTGG CATGCCAGAA ATTGATGTGA GCGATTGGAT AAGAAATACA
    GAATACACAA GTGGCTATGA AAGAGAAGAT CCAGTTATTC AGTGGTTCTG GGAAGTTGTG
    GAAGGCATTA CTCCAGAGGA GAGAGTTCTT CTCTTGCAGT TTGTTACTGG CAGTTCCAGG
    GTCCCACATG GTGGATTTGC TAACATCATG GGTGGAAGTG GATTGCAAAA CTTTACAATT
    GCTGCTGTGC CATATACTCC AAATCTCCTA CCAACTTCAA GCACATGCAT CAACATGCTC
    AAGTTACCTG AATACCCAAG TAAAGAAATA CTCAAGGACA GACTTCTTGT GGCGTTACAT
    TGTGGCAGCT ATGGTTACAC AATGGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_854232.1
    CDS80..2809
    Translation

    Target ORF information:

    RefSeq Version XM_849139.5
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_849139.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAGAGAG CGATGGAGCA GCTCAACCGC CTCACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CGGACGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT
    GATCAACACA GGTCTGTTTC TGAGCTACTA TCAAATTCAA AGTTTGATGT CAATTATGCT
    TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC
    TTGGTCCTGC TATTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC AGGATGTACA
    CCCCTTCATT TGGCAGCTAG AAATGGGCAG AAGAAATGCA TGAGTAAATT ATTAGAATAC
    AGTGCTGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAATACACTG GCTCGCTGTG
    AATGGGCGGA CAGAACTACT CCATGACCTT GTGCAGCACG TCAGTGACGT CGACGTTGAG
    GATGCGATGG GGCAGACAGC ACTGCATGTC GCCTGCCAGA ATGGTCACAA GACGACAGTG
    CAGTGCTTGC TAGACAGTGG TGCTGATATT AACAGGCCAA ATGTATCTGG AGCCACTCCA
    TTGTACTTTG CTTGCAGTCA TGGTCAGAGA GACACAGCAC AAATTCTTCT GTTACGAGGA
    GCTAAATATC TGTCAGATAA AAATGGAGTA ACGCCTCTGG ATTTATGTGT ACAGGGTGGC
    TATGGAGAGA CTTGTGAAGT ATTAATTCAG TATCACCCTA GGCTTTTCCA GACAATTATT
    CAAATGACAC AGAATGAAGA TCTCCGAGAA AACATGTTAC GGCAAGTTCT GGAACATTTG
    TCTCAGCAAA GTGAAAGCCA GTACCTAAAG ATTCTAACAA GCCTTGCTGA AGTTGCCACA
    ACAAATGGGC ATAAACTGCT TAGTCTGTCT AGTAATTATG ATGCTCAGAT GAAGAGCCTC
    TTAAGGATTG TGAGGATATT TTGTCATGTC TTTCGAATTG GTCCATCCTC TCCCAGTAAT
    GGAATTGATT TGGGCTACAA CGGGAATAAA ACTCCAAGAA GCCAAGTGTT CAAGCCTCTT
    GAATTGCTTT GGCACTCATT AGATGAATGG CTAGTTTTAA TAGCTACAGA ATTGATGAAA
    AACAAAAAGG ACTCAACGGA TATCACTTCT ATTTTACTGA AACAAAAAGG CCAAGATCAA
    GATGGTACTT CCATTCCTCC ATTTGAACCT CCAGGACCTG GGAGCTATGA AAATCTATCC
    AGTGGCACAG GAGAATCTAA ACCAGATGCT CTTGGAGGGA AACAGGAAGC CAGTGCAGAC
    TGTCAGGATG TTATTTCTAT GACAGCTAAC CGGCTAAGTG CTGTCATTCA AGCTTTTTAC
    ATGTGCTGTT CTTGTCAGAT GCCTCCAGGA ATGACCTCAC CCCGTTTCAT TGAATTTGTC
    TGCAAACATG ATGAAGTTTT AAAATGCTTT GTTAATAGAA ATCCCAAAAT TATATTTGAT
    CACTTTCATT TCCTCCTTGA ATGTCCTGAG TTGATGTCAA GATTCATGCA TATCATAAAA
    GCACAGCCAT TTAAAGATCG CTGTGAGTGG TTCTATGAAC ATTTGCACTC AGGACAACCA
    GATTCTGACA TGGTGCACAG GCCAGTGAAT GAAAATGATA TCCTGCTTGT TCACAGAGAT
    TCTATTTTTA GGAGTAGCTG TGAAGTTGTG TCAAAAGCAA ACTGTGCAAA GCTAAAGCAA
    GGAATTGCTG TACGGTTCCA TGGAGAAGAA GGCATGGGCC AAGGTGTTGT GCGTGAGTGG
    TTTGATATTC TGTCTAATGA GATAGTGAAT CCTGATTATG CATTGTTCAC CCAGTCAGCT
    GATGGAACAA CTTTTCAGCC TAATAGTAAT TCCTATGTAA ATCCTGATCA CTTGAACTAT
    TTCCGGTTTG CTGGACAGAT CTTGGGATTA GCTTTGAACC ACAGGCAGCT GGTCAACATT
    TACTTCACAA GATCGTTCTA TAAGCACATT CTTGGTATCC CTGTAAATTA CCAAGATGTG
    GCATCCATTG ATCCAGAATA TGCCAAAAAT TTGCAGTGGA TTTTAGATAA TGACATTAGT
    GATCTGGGTC TAGAACTAAC CTTTTCTGTT GAAACTGATG TGTTTGGAGC AATGGAAGAG
    GTGCCTTTGA AACCTGGGGG TGGAAGTATT CTTGTGACAC AAAATAACAA AGCGGAGTAT
    GTCCAGCTTG TTACTGAACT TCGAATGACA AGAGCCATTC AACCTCAGAT CAATGCTTTT
    TTACAGGGCT TTCATATGTT CATTCCACCT TCCCTCATAC AACTTTTTGA TGAATATGAA
    TTGGAGCTAC TGCTTTCTGG CATGCCAGAA ATTGATGTGA GCGATTGGAT AAGAAATACA
    GAATACACAA GTGGCTATGA AAGAGAAGAT CCAGTTATTC AGTGGTTCTG GGAAGTTGTG
    GAAGGCATTA CTCCAGAGGA GAGAGTTCTT CTCTTGCAGT TTGTTACTGG CAGTTCCAGG
    GTCCCACATG GTGGATTTGC TAACATCATG GGTGGAAGTG GATTGCAAAA CTTTACAATT
    GCTGCTGTGC CATATACTCC AAATCTCCTA CCAACTTCAA GCACATGCAT CAACATGCTC
    AAGTTACCTG AATACCCAAG TAAAGAAATA CTCAAGGACA GACTTCTTGT GGCGTTACAT
    TGTGGCAGCT ATGGTTACAC AATGGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe15638
    Clone ID Related Accession (Same CDS sequence) XM_005627667.2 , XM_005627667.3
    Accession Version XM_005627667.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2598bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase HACE1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005627667.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGAGAG CGATGGAGCA GCTCAACCGC CTCACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CGGACGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT
    GATCAACACA GGTCTGTTTC TGAGCTACTA TCAAATTCAA AGTTTGATGT CAATTATGCT
    TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC
    TTGGTCCTGC TATTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC AGGATGTACA
    CCCCTTCATT TGGCAGCTAG AAATGGGCAG AAGAAATGCA TGAGTAAATT ATTAGAATAC
    AGTGCTGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAACAGTGCA GTGCTTGCTA
    GACAGTGGTG CTGATATTAA CAGGCCAAAT GTATCTGGAG CCACTCCATT GTACTTTGCT
    TGCAGTCATG GTCAGAGAGA CACAGCACAA ATTCTTCTGT TACGAGGAGC TAAATATCTG
    TCAGATAAAA ATGGAGTAAC GCCTCTGGAT TTATGTGTAC AGGGTGGCTA TGGAGAGACT
    TGTGAAGTAT TAATTCAGTA TCACCCTAGG CTTTTCCAGA CAATTATTCA AATGACACAG
    AATGAAGATC TCCGAGAAAA CATGTTACGG CAAGTTCTGG AACATTTGTC TCAGCAAAGT
    GAAAGCCAGT ACCTAAAGAT TCTAACAAGC CTTGCTGAAG TTGCCACAAC AAATGGGCAT
    AAACTGCTTA GTCTGTCTAG TAATTATGAT GCTCAGATGA AGAGCCTCTT AAGGATTGTG
    AGGATATTTT GTCATGTCTT TCGAATTGGT CCATCCTCTC CCAGTAATGG AATTGATTTG
    GGCTACAACG GGAATAAAAC TCCAAGAAGC CAAGTGTTCA AGCCTCTTGA ATTGCTTTGG
    CACTCATTAG ATGAATGGCT AGTTTTAATA GCTACAGAAT TGATGAAAAA CAAAAAGGAC
    TCAACGGATA TCACTTCTAT TTTACTGAAA CAAAAAGGCC AAGATCAAGA TGGTACTTCC
    ATTCCTCCAT TTGAACCTCC AGGACCTGGG AGCTATGAAA ATCTATCCAG TGGCACAGGA
    GAATCTAAAC CAGATGCTCT TGGAGGGAAA CAGGAAGCCA GTGCAGACTG TCAGGATGTT
    ATTTCTATGA CAGCTAACCG GCTAAGTGCT GTCATTCAAG CTTTTTACAT GTGCTGTTCT
    TGTCAGATGC CTCCAGGAAT GACCTCACCC CGTTTCATTG AATTTGTCTG CAAACATGAT
    GAAGTTTTAA AATGCTTTGT TAATAGAAAT CCCAAAATTA TATTTGATCA CTTTCATTTC
    CTCCTTGAAT GTCCTGAGTT GATGTCAAGA TTCATGCATA TCATAAAAGC ACAGCCATTT
    AAAGATCGCT GTGAGTGGTT CTATGAACAT TTGCACTCAG GACAACCAGA TTCTGACATG
    GTGCACAGGC CAGTGAATGA AAATGATATC CTGCTTGTTC ACAGAGATTC TATTTTTAGG
    AGTAGCTGTG AAGTTGTGTC AAAAGCAAAC TGTGCAAAGC TAAAGCAAGG AATTGCTGTA
    CGGTTCCATG GAGAAGAAGG CATGGGCCAA GGTGTTGTGC GTGAGTGGTT TGATATTCTG
    TCTAATGAGA TAGTGAATCC TGATTATGCA TTGTTCACCC AGTCAGCTGA TGGAACAACT
    TTTCAGCCTA ATAGTAATTC CTATGTAAAT CCTGATCACT TGAACTATTT CCGGTTTGCT
    GGACAGATCT TGGGATTAGC TTTGAACCAC AGGCAGCTGG TCAACATTTA CTTCACAAGA
    TCGTTCTATA AGCACATTCT TGGTATCCCT GTAAATTACC AAGATGTGGC ATCCATTGAT
    CCAGAATATG CCAAAAATTT GCAGTGGATT TTAGATAATG ACATTAGTGA TCTGGGTCTA
    GAACTAACCT TTTCTGTTGA AACTGATGTG TTTGGAGCAA TGGAAGAGGT GCCTTTGAAA
    CCTGGGGGTG GAAGTATTCT TGTGACACAA AATAACAAAG CGGAGTATGT CCAGCTTGTT
    ACTGAACTTC GAATGACAAG AGCCATTCAA CCTCAGATCA ATGCTTTTTT ACAGGGCTTT
    CATATGTTCA TTCCACCTTC CCTCATACAA CTTTTTGATG AATATGAATT GGAGCTACTG
    CTTTCTGGCA TGCCAGAAAT TGATGTGAGC GATTGGATAA GAAATACAGA ATACACAAGT
    GGCTATGAAA GAGAAGATCC AGTTATTCAG TGGTTCTGGG AAGTTGTGGA AGGCATTACT
    CCAGAGGAGA GAGTTCTTCT CTTGCAGTTT GTTACTGGCA GTTCCAGGGT CCCACATGGT
    GGATTTGCTA ACATCATGGG TGGAAGTGGA TTGCAAAACT TTACAATTGC TGCTGTGCCA
    TATACTCCAA ATCTCCTACC AACTTCAAGC ACATGCATCA ACATGCTCAA GTTACCTGAA
    TACCCAAGTA AAGAAATACT CAAGGACAGA CTTCTTGTGG CGTTACATTG TGGCAGCTAT
    GGTTACACAA TGGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005627724.1
    CDS190..2787
    Translation

    Target ORF information:

    RefSeq Version XM_005627667.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005627667.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGAGAG CGATGGAGCA GCTCAACCGC CTCACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CGGACGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT
    GATCAACACA GGTCTGTTTC TGAGCTACTA TCAAATTCAA AGTTTGATGT CAATTATGCT
    TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC
    TTGGTCCTGC TATTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC AGGATGTACA
    CCCCTTCATT TGGCAGCTAG AAATGGGCAG AAGAAATGCA TGAGTAAATT ATTAGAATAC
    AGTGCTGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAACAGTGCA GTGCTTGCTA
    GACAGTGGTG CTGATATTAA CAGGCCAAAT GTATCTGGAG CCACTCCATT GTACTTTGCT
    TGCAGTCATG GTCAGAGAGA CACAGCACAA ATTCTTCTGT TACGAGGAGC TAAATATCTG
    TCAGATAAAA ATGGAGTAAC GCCTCTGGAT TTATGTGTAC AGGGTGGCTA TGGAGAGACT
    TGTGAAGTAT TAATTCAGTA TCACCCTAGG CTTTTCCAGA CAATTATTCA AATGACACAG
    AATGAAGATC TCCGAGAAAA CATGTTACGG CAAGTTCTGG AACATTTGTC TCAGCAAAGT
    GAAAGCCAGT ACCTAAAGAT TCTAACAAGC CTTGCTGAAG TTGCCACAAC AAATGGGCAT
    AAACTGCTTA GTCTGTCTAG TAATTATGAT GCTCAGATGA AGAGCCTCTT AAGGATTGTG
    AGGATATTTT GTCATGTCTT TCGAATTGGT CCATCCTCTC CCAGTAATGG AATTGATTTG
    GGCTACAACG GGAATAAAAC TCCAAGAAGC CAAGTGTTCA AGCCTCTTGA ATTGCTTTGG
    CACTCATTAG ATGAATGGCT AGTTTTAATA GCTACAGAAT TGATGAAAAA CAAAAAGGAC
    TCAACGGATA TCACTTCTAT TTTACTGAAA CAAAAAGGCC AAGATCAAGA TGGTACTTCC
    ATTCCTCCAT TTGAACCTCC AGGACCTGGG AGCTATGAAA ATCTATCCAG TGGCACAGGA
    GAATCTAAAC CAGATGCTCT TGGAGGGAAA CAGGAAGCCA GTGCAGACTG TCAGGATGTT
    ATTTCTATGA CAGCTAACCG GCTAAGTGCT GTCATTCAAG CTTTTTACAT GTGCTGTTCT
    TGTCAGATGC CTCCAGGAAT GACCTCACCC CGTTTCATTG AATTTGTCTG CAAACATGAT
    GAAGTTTTAA AATGCTTTGT TAATAGAAAT CCCAAAATTA TATTTGATCA CTTTCATTTC
    CTCCTTGAAT GTCCTGAGTT GATGTCAAGA TTCATGCATA TCATAAAAGC ACAGCCATTT
    AAAGATCGCT GTGAGTGGTT CTATGAACAT TTGCACTCAG GACAACCAGA TTCTGACATG
    GTGCACAGGC CAGTGAATGA AAATGATATC CTGCTTGTTC ACAGAGATTC TATTTTTAGG
    AGTAGCTGTG AAGTTGTGTC AAAAGCAAAC TGTGCAAAGC TAAAGCAAGG AATTGCTGTA
    CGGTTCCATG GAGAAGAAGG CATGGGCCAA GGTGTTGTGC GTGAGTGGTT TGATATTCTG
    TCTAATGAGA TAGTGAATCC TGATTATGCA TTGTTCACCC AGTCAGCTGA TGGAACAACT
    TTTCAGCCTA ATAGTAATTC CTATGTAAAT CCTGATCACT TGAACTATTT CCGGTTTGCT
    GGACAGATCT TGGGATTAGC TTTGAACCAC AGGCAGCTGG TCAACATTTA CTTCACAAGA
    TCGTTCTATA AGCACATTCT TGGTATCCCT GTAAATTACC AAGATGTGGC ATCCATTGAT
    CCAGAATATG CCAAAAATTT GCAGTGGATT TTAGATAATG ACATTAGTGA TCTGGGTCTA
    GAACTAACCT TTTCTGTTGA AACTGATGTG TTTGGAGCAA TGGAAGAGGT GCCTTTGAAA
    CCTGGGGGTG GAAGTATTCT TGTGACACAA AATAACAAAG CGGAGTATGT CCAGCTTGTT
    ACTGAACTTC GAATGACAAG AGCCATTCAA CCTCAGATCA ATGCTTTTTT ACAGGGCTTT
    CATATGTTCA TTCCACCTTC CCTCATACAA CTTTTTGATG AATATGAATT GGAGCTACTG
    CTTTCTGGCA TGCCAGAAAT TGATGTGAGC GATTGGATAA GAAATACAGA ATACACAAGT
    GGCTATGAAA GAGAAGATCC AGTTATTCAG TGGTTCTGGG AAGTTGTGGA AGGCATTACT
    CCAGAGGAGA GAGTTCTTCT CTTGCAGTTT GTTACTGGCA GTTCCAGGGT CCCACATGGT
    GGATTTGCTA ACATCATGGG TGGAAGTGGA TTGCAAAACT TTACAATTGC TGCTGTGCCA
    TATACTCCAA ATCTCCTACC AACTTCAAGC ACATGCATCA ACATGCTCAA GTTACCTGAA
    TACCCAAGTA AAGAAATACT CAAGGACAGA CTTCTTGTGG CGTTACATTG TGGCAGCTAT
    GGTTACACAA TGGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe15638
    Clone ID Related Accession (Same CDS sequence) XM_005627667.2 , XM_005627667.3
    Accession Version XM_005627667.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2598bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase HACE1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005627667.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGAGAG CGATGGAGCA GCTCAACCGC CTCACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CGGACGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT
    GATCAACACA GGTCTGTTTC TGAGCTACTA TCAAATTCAA AGTTTGATGT CAATTATGCT
    TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC
    TTGGTCCTGC TATTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC AGGATGTACA
    CCCCTTCATT TGGCAGCTAG AAATGGGCAG AAGAAATGCA TGAGTAAATT ATTAGAATAC
    AGTGCTGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAACAGTGCA GTGCTTGCTA
    GACAGTGGTG CTGATATTAA CAGGCCAAAT GTATCTGGAG CCACTCCATT GTACTTTGCT
    TGCAGTCATG GTCAGAGAGA CACAGCACAA ATTCTTCTGT TACGAGGAGC TAAATATCTG
    TCAGATAAAA ATGGAGTAAC GCCTCTGGAT TTATGTGTAC AGGGTGGCTA TGGAGAGACT
    TGTGAAGTAT TAATTCAGTA TCACCCTAGG CTTTTCCAGA CAATTATTCA AATGACACAG
    AATGAAGATC TCCGAGAAAA CATGTTACGG CAAGTTCTGG AACATTTGTC TCAGCAAAGT
    GAAAGCCAGT ACCTAAAGAT TCTAACAAGC CTTGCTGAAG TTGCCACAAC AAATGGGCAT
    AAACTGCTTA GTCTGTCTAG TAATTATGAT GCTCAGATGA AGAGCCTCTT AAGGATTGTG
    AGGATATTTT GTCATGTCTT TCGAATTGGT CCATCCTCTC CCAGTAATGG AATTGATTTG
    GGCTACAACG GGAATAAAAC TCCAAGAAGC CAAGTGTTCA AGCCTCTTGA ATTGCTTTGG
    CACTCATTAG ATGAATGGCT AGTTTTAATA GCTACAGAAT TGATGAAAAA CAAAAAGGAC
    TCAACGGATA TCACTTCTAT TTTACTGAAA CAAAAAGGCC AAGATCAAGA TGGTACTTCC
    ATTCCTCCAT TTGAACCTCC AGGACCTGGG AGCTATGAAA ATCTATCCAG TGGCACAGGA
    GAATCTAAAC CAGATGCTCT TGGAGGGAAA CAGGAAGCCA GTGCAGACTG TCAGGATGTT
    ATTTCTATGA CAGCTAACCG GCTAAGTGCT GTCATTCAAG CTTTTTACAT GTGCTGTTCT
    TGTCAGATGC CTCCAGGAAT GACCTCACCC CGTTTCATTG AATTTGTCTG CAAACATGAT
    GAAGTTTTAA AATGCTTTGT TAATAGAAAT CCCAAAATTA TATTTGATCA CTTTCATTTC
    CTCCTTGAAT GTCCTGAGTT GATGTCAAGA TTCATGCATA TCATAAAAGC ACAGCCATTT
    AAAGATCGCT GTGAGTGGTT CTATGAACAT TTGCACTCAG GACAACCAGA TTCTGACATG
    GTGCACAGGC CAGTGAATGA AAATGATATC CTGCTTGTTC ACAGAGATTC TATTTTTAGG
    AGTAGCTGTG AAGTTGTGTC AAAAGCAAAC TGTGCAAAGC TAAAGCAAGG AATTGCTGTA
    CGGTTCCATG GAGAAGAAGG CATGGGCCAA GGTGTTGTGC GTGAGTGGTT TGATATTCTG
    TCTAATGAGA TAGTGAATCC TGATTATGCA TTGTTCACCC AGTCAGCTGA TGGAACAACT
    TTTCAGCCTA ATAGTAATTC CTATGTAAAT CCTGATCACT TGAACTATTT CCGGTTTGCT
    GGACAGATCT TGGGATTAGC TTTGAACCAC AGGCAGCTGG TCAACATTTA CTTCACAAGA
    TCGTTCTATA AGCACATTCT TGGTATCCCT GTAAATTACC AAGATGTGGC ATCCATTGAT
    CCAGAATATG CCAAAAATTT GCAGTGGATT TTAGATAATG ACATTAGTGA TCTGGGTCTA
    GAACTAACCT TTTCTGTTGA AACTGATGTG TTTGGAGCAA TGGAAGAGGT GCCTTTGAAA
    CCTGGGGGTG GAAGTATTCT TGTGACACAA AATAACAAAG CGGAGTATGT CCAGCTTGTT
    ACTGAACTTC GAATGACAAG AGCCATTCAA CCTCAGATCA ATGCTTTTTT ACAGGGCTTT
    CATATGTTCA TTCCACCTTC CCTCATACAA CTTTTTGATG AATATGAATT GGAGCTACTG
    CTTTCTGGCA TGCCAGAAAT TGATGTGAGC GATTGGATAA GAAATACAGA ATACACAAGT
    GGCTATGAAA GAGAAGATCC AGTTATTCAG TGGTTCTGGG AAGTTGTGGA AGGCATTACT
    CCAGAGGAGA GAGTTCTTCT CTTGCAGTTT GTTACTGGCA GTTCCAGGGT CCCACATGGT
    GGATTTGCTA ACATCATGGG TGGAAGTGGA TTGCAAAACT TTACAATTGC TGCTGTGCCA
    TATACTCCAA ATCTCCTACC AACTTCAAGC ACATGCATCA ACATGCTCAA GTTACCTGAA
    TACCCAAGTA AAGAAATACT CAAGGACAGA CTTCTTGTGG CGTTACATTG TGGCAGCTAT
    GGTTACACAA TGGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005627724.1
    CDS80..2677
    Translation

    Target ORF information:

    RefSeq Version XM_005627667.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005627667.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGAGAG CGATGGAGCA GCTCAACCGC CTCACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CGGACGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT
    GATCAACACA GGTCTGTTTC TGAGCTACTA TCAAATTCAA AGTTTGATGT CAATTATGCT
    TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC
    TTGGTCCTGC TATTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC AGGATGTACA
    CCCCTTCATT TGGCAGCTAG AAATGGGCAG AAGAAATGCA TGAGTAAATT ATTAGAATAC
    AGTGCTGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAACAGTGCA GTGCTTGCTA
    GACAGTGGTG CTGATATTAA CAGGCCAAAT GTATCTGGAG CCACTCCATT GTACTTTGCT
    TGCAGTCATG GTCAGAGAGA CACAGCACAA ATTCTTCTGT TACGAGGAGC TAAATATCTG
    TCAGATAAAA ATGGAGTAAC GCCTCTGGAT TTATGTGTAC AGGGTGGCTA TGGAGAGACT
    TGTGAAGTAT TAATTCAGTA TCACCCTAGG CTTTTCCAGA CAATTATTCA AATGACACAG
    AATGAAGATC TCCGAGAAAA CATGTTACGG CAAGTTCTGG AACATTTGTC TCAGCAAAGT
    GAAAGCCAGT ACCTAAAGAT TCTAACAAGC CTTGCTGAAG TTGCCACAAC AAATGGGCAT
    AAACTGCTTA GTCTGTCTAG TAATTATGAT GCTCAGATGA AGAGCCTCTT AAGGATTGTG
    AGGATATTTT GTCATGTCTT TCGAATTGGT CCATCCTCTC CCAGTAATGG AATTGATTTG
    GGCTACAACG GGAATAAAAC TCCAAGAAGC CAAGTGTTCA AGCCTCTTGA ATTGCTTTGG
    CACTCATTAG ATGAATGGCT AGTTTTAATA GCTACAGAAT TGATGAAAAA CAAAAAGGAC
    TCAACGGATA TCACTTCTAT TTTACTGAAA CAAAAAGGCC AAGATCAAGA TGGTACTTCC
    ATTCCTCCAT TTGAACCTCC AGGACCTGGG AGCTATGAAA ATCTATCCAG TGGCACAGGA
    GAATCTAAAC CAGATGCTCT TGGAGGGAAA CAGGAAGCCA GTGCAGACTG TCAGGATGTT
    ATTTCTATGA CAGCTAACCG GCTAAGTGCT GTCATTCAAG CTTTTTACAT GTGCTGTTCT
    TGTCAGATGC CTCCAGGAAT GACCTCACCC CGTTTCATTG AATTTGTCTG CAAACATGAT
    GAAGTTTTAA AATGCTTTGT TAATAGAAAT CCCAAAATTA TATTTGATCA CTTTCATTTC
    CTCCTTGAAT GTCCTGAGTT GATGTCAAGA TTCATGCATA TCATAAAAGC ACAGCCATTT
    AAAGATCGCT GTGAGTGGTT CTATGAACAT TTGCACTCAG GACAACCAGA TTCTGACATG
    GTGCACAGGC CAGTGAATGA AAATGATATC CTGCTTGTTC ACAGAGATTC TATTTTTAGG
    AGTAGCTGTG AAGTTGTGTC AAAAGCAAAC TGTGCAAAGC TAAAGCAAGG AATTGCTGTA
    CGGTTCCATG GAGAAGAAGG CATGGGCCAA GGTGTTGTGC GTGAGTGGTT TGATATTCTG
    TCTAATGAGA TAGTGAATCC TGATTATGCA TTGTTCACCC AGTCAGCTGA TGGAACAACT
    TTTCAGCCTA ATAGTAATTC CTATGTAAAT CCTGATCACT TGAACTATTT CCGGTTTGCT
    GGACAGATCT TGGGATTAGC TTTGAACCAC AGGCAGCTGG TCAACATTTA CTTCACAAGA
    TCGTTCTATA AGCACATTCT TGGTATCCCT GTAAATTACC AAGATGTGGC ATCCATTGAT
    CCAGAATATG CCAAAAATTT GCAGTGGATT TTAGATAATG ACATTAGTGA TCTGGGTCTA
    GAACTAACCT TTTCTGTTGA AACTGATGTG TTTGGAGCAA TGGAAGAGGT GCCTTTGAAA
    CCTGGGGGTG GAAGTATTCT TGTGACACAA AATAACAAAG CGGAGTATGT CCAGCTTGTT
    ACTGAACTTC GAATGACAAG AGCCATTCAA CCTCAGATCA ATGCTTTTTT ACAGGGCTTT
    CATATGTTCA TTCCACCTTC CCTCATACAA CTTTTTGATG AATATGAATT GGAGCTACTG
    CTTTCTGGCA TGCCAGAAAT TGATGTGAGC GATTGGATAA GAAATACAGA ATACACAAGT
    GGCTATGAAA GAGAAGATCC AGTTATTCAG TGGTTCTGGG AAGTTGTGGA AGGCATTACT
    CCAGAGGAGA GAGTTCTTCT CTTGCAGTTT GTTACTGGCA GTTCCAGGGT CCCACATGGT
    GGATTTGCTA ACATCATGGG TGGAAGTGGA TTGCAAAACT TTACAATTGC TGCTGTGCCA
    TATACTCCAA ATCTCCTACC AACTTCAAGC ACATGCATCA ACATGCTCAA GTTACCTGAA
    TACCCAAGTA AAGAAATACT CAAGGACAGA CTTCTTGTGG CGTTACATTG TGGCAGCTAT
    GGTTACACAA TGGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe292658
    Clone ID Related Accession (Same CDS sequence) XM_022426399.1
    Accession Version XM_022426399.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2628bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase HACE1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCCAATGG TTATGGCTGA TCAACACAGG TCTGTTTCTG AGCTACTATC AAATTCAAAG 
    TTTGATGTCA ATTATGCTTT TGGACGTGTG AAAAGAAGCT TGCTTCACAT TGCAGCAAAT
    TGTGGATCAG TGGAATGCTT GGTCCTGCTA TTAAAGAAAG GAGCAAATCC TAACTATCAA
    GATATTTCAG GATGTACACC CCTTCATTTG GCAGCTAGAA ATGGGCAGAA GAAATGCATG
    AGTAAATTAT TAGAATACAG TGCTGATGTC AACATTTGTA ATAATGAAGG CCTTACAGCA
    ATACACTGGC TCGCTGTGAA TGGGCGGACA GAACTACTCC ATGACCTTGT GCAGCACGTC
    AGTGACGTCG ACGTTGAGGA TGCGATGGGG CAGACAGCAC TGCATGTCGC CTGCCAGAAT
    GGTCACAAGA CGACAGTGCA GTGCTTGCTA GACAGTGGTG CTGATATTAA CAGGCCAAAT
    GTATCTGGAG CCACTCCATT GTACTTTGCT TGCAGTCATG GTCAGAGAGA CACAGCACAA
    ATTCTTCTGT TACGAGGAGC TAAATATCTG TCAGATAAAA ATGGAGTAAC GCCTCTGGAT
    TTATGTGTAC AGGGTGGCTA TGGAGAGACT TGTGAAGTAT TAATTCAGTA TCACCCTAGG
    CTTTTCCAGA CAATTATTCA AATGACACAG AATGAAGATC TCCGAGAAAA CATGTTACGG
    CAAGTTCTGG AACATTTGTC TCAGCAAAGT GAAAGCCAGT ACCTAAAGAT TCTAACAAGC
    CTTGCTGAAG TTGCCACAAC AAATGGGCAT AAACTGCTTA GTCTGTCTAG TAATTATGAT
    GCTCAGATGA AGAGCCTCTT AAGGATTGTG AGGATATTTT GTCATGTCTT TCGAATTGGT
    CCATCCTCTC CCAGTAATGG AATTGATTTG GGCTACAACG GGAATAAAAC TCCAAGAAGC
    CAAGTGTTCA AGCCTCTTGA ATTGCTTTGG CACTCATTAG ATGAATGGCT AGTTTTAATA
    GCTACAGAAT TGATGAAAAA CAAAAAGGAC TCAACGGATA TCACTTCTAT TTTACTGAAA
    CAAAAAGGCC AAGATCAAGA TGGTACTTCC ATTCCTCCAT TTGAACCTCC AGGACCTGGG
    AGCTATGAAA ATCTATCCAG TGGCACAGGA GAATCTAAAC CAGATGCTCT TGGAGGGAAA
    CAGGAAGCCA GTGCAGACTG TCAGGATGTT ATTTCTATGA CAGCTAACCG GCTAAGTGCT
    GTCATTCAAG CTTTTTACAT GTGCTGTTCT TGTCAGATGC CTCCAGGAAT GACCTCACCC
    CGTTTCATTG AATTTGTCTG CAAACATGAT GAAGTTTTAA AATGCTTTGT TAATAGAAAT
    CCCAAAATTA TATTTGATCA CTTTCATTTC CTCCTTGAAT GTCCTGAGTT GATGTCAAGA
    TTCATGCATA TCATAAAAGC ACAGCCATTT AAAGATCGCT GTGAGTGGTT CTATGAACAT
    TTGCACTCAG GACAACCAGA TTCTGACATG GTGCACAGGC CAGTGAATGA AAATGATATC
    CTGCTTGTTC ACAGAGATTC TATTTTTAGG AGTAGCTGTG AAGTTGTGTC AAAAGCAAAC
    TGTGCAAAGC TAAAGCAAGG AATTGCTGTA CGGTTCCATG GAGAAGAAGG CATGGGCCAA
    GGTGTTGTGC GTGAGTGGTT TGATATTCTG TCTAATGAGA TAGTGAATCC TGATTATGCA
    TTGTTCACCC AGTCAGCTGA TGGAACAACT TTTCAGCCTA ATAGTAATTC CTATGTAAAT
    CCTGATCACT TGAACTATTT CCGGTTTGCT GGACAGATCT TGGGATTAGC TTTGAACCAC
    AGGCAGCTGG TCAACATTTA CTTCACAAGA TCGTTCTATA AGCACATTCT TGGTATCCCT
    GTAAATTACC AAGATGTGGC ATCCATTGAT CCAGAATATG CCAAAAATTT GCAGTGGATT
    TTAGATAATG ACATTAGTGA TCTGGGTCTA GAACTAACCT TTTCTGTTGA AACTGATGTG
    TTTGGAGCAA TGGAAGAGGT GCCTTTGAAA CCTGGGGGTG GAAGTATTCT TGTGACACAA
    AATAACAAAG CGGAGTATGT CCAGCTTGTT ACTGAACTTC GAATGACAAG AGCCATTCAA
    CCTCAGATCA ATGCTTTTTT ACAGGGCTTT CATATGTTCA TTCCACCTTC CCTCATACAA
    CTTTTTGATG AATATGAATT GGAGCTACTG CTTTCTGGCA TGCCAGAAAT TGATGTGAGC
    GATTGGATAA GAAATACAGA ATACACAAGT GGCTATGAAA GAGAAGATCC AGTTATTCAG
    TGGTTCTGGG AAGTTGTGGA AGGCATTACT CCAGAGGAGA GAGTTCTTCT CTTGCAGTTT
    GTTACTGGCA GTTCCAGGGT CCCACATGGT GGATTTGCTA ACATCATGGG TGGAAGTGGA
    TTGCAAAACT TTACAATTGC TGCTGTGCCA TATACTCCAA ATCTCCTACC AACTTCAAGC
    ACATGCATCA ACATGCTCAA GTTACCTGAA TACCCAAGTA AAGAAATACT CAAGGACAGA
    CTTCTTGTGG CGTTACATTG TGGCAGCTAT GGTTACACAA TGGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022282107.1
    CDS46..2673
    Translation

    Target ORF information:

    RefSeq Version XM_022426399.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426399.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCCAATGG TTATGGCTGA TCAACACAGG TCTGTTTCTG AGCTACTATC AAATTCAAAG 
    TTTGATGTCA ATTATGCTTT TGGACGTGTG AAAAGAAGCT TGCTTCACAT TGCAGCAAAT
    TGTGGATCAG TGGAATGCTT GGTCCTGCTA TTAAAGAAAG GAGCAAATCC TAACTATCAA
    GATATTTCAG GATGTACACC CCTTCATTTG GCAGCTAGAA ATGGGCAGAA GAAATGCATG
    AGTAAATTAT TAGAATACAG TGCTGATGTC AACATTTGTA ATAATGAAGG CCTTACAGCA
    ATACACTGGC TCGCTGTGAA TGGGCGGACA GAACTACTCC ATGACCTTGT GCAGCACGTC
    AGTGACGTCG ACGTTGAGGA TGCGATGGGG CAGACAGCAC TGCATGTCGC CTGCCAGAAT
    GGTCACAAGA CGACAGTGCA GTGCTTGCTA GACAGTGGTG CTGATATTAA CAGGCCAAAT
    GTATCTGGAG CCACTCCATT GTACTTTGCT TGCAGTCATG GTCAGAGAGA CACAGCACAA
    ATTCTTCTGT TACGAGGAGC TAAATATCTG TCAGATAAAA ATGGAGTAAC GCCTCTGGAT
    TTATGTGTAC AGGGTGGCTA TGGAGAGACT TGTGAAGTAT TAATTCAGTA TCACCCTAGG
    CTTTTCCAGA CAATTATTCA AATGACACAG AATGAAGATC TCCGAGAAAA CATGTTACGG
    CAAGTTCTGG AACATTTGTC TCAGCAAAGT GAAAGCCAGT ACCTAAAGAT TCTAACAAGC
    CTTGCTGAAG TTGCCACAAC AAATGGGCAT AAACTGCTTA GTCTGTCTAG TAATTATGAT
    GCTCAGATGA AGAGCCTCTT AAGGATTGTG AGGATATTTT GTCATGTCTT TCGAATTGGT
    CCATCCTCTC CCAGTAATGG AATTGATTTG GGCTACAACG GGAATAAAAC TCCAAGAAGC
    CAAGTGTTCA AGCCTCTTGA ATTGCTTTGG CACTCATTAG ATGAATGGCT AGTTTTAATA
    GCTACAGAAT TGATGAAAAA CAAAAAGGAC TCAACGGATA TCACTTCTAT TTTACTGAAA
    CAAAAAGGCC AAGATCAAGA TGGTACTTCC ATTCCTCCAT TTGAACCTCC AGGACCTGGG
    AGCTATGAAA ATCTATCCAG TGGCACAGGA GAATCTAAAC CAGATGCTCT TGGAGGGAAA
    CAGGAAGCCA GTGCAGACTG TCAGGATGTT ATTTCTATGA CAGCTAACCG GCTAAGTGCT
    GTCATTCAAG CTTTTTACAT GTGCTGTTCT TGTCAGATGC CTCCAGGAAT GACCTCACCC
    CGTTTCATTG AATTTGTCTG CAAACATGAT GAAGTTTTAA AATGCTTTGT TAATAGAAAT
    CCCAAAATTA TATTTGATCA CTTTCATTTC CTCCTTGAAT GTCCTGAGTT GATGTCAAGA
    TTCATGCATA TCATAAAAGC ACAGCCATTT AAAGATCGCT GTGAGTGGTT CTATGAACAT
    TTGCACTCAG GACAACCAGA TTCTGACATG GTGCACAGGC CAGTGAATGA AAATGATATC
    CTGCTTGTTC ACAGAGATTC TATTTTTAGG AGTAGCTGTG AAGTTGTGTC AAAAGCAAAC
    TGTGCAAAGC TAAAGCAAGG AATTGCTGTA CGGTTCCATG GAGAAGAAGG CATGGGCCAA
    GGTGTTGTGC GTGAGTGGTT TGATATTCTG TCTAATGAGA TAGTGAATCC TGATTATGCA
    TTGTTCACCC AGTCAGCTGA TGGAACAACT TTTCAGCCTA ATAGTAATTC CTATGTAAAT
    CCTGATCACT TGAACTATTT CCGGTTTGCT GGACAGATCT TGGGATTAGC TTTGAACCAC
    AGGCAGCTGG TCAACATTTA CTTCACAAGA TCGTTCTATA AGCACATTCT TGGTATCCCT
    GTAAATTACC AAGATGTGGC ATCCATTGAT CCAGAATATG CCAAAAATTT GCAGTGGATT
    TTAGATAATG ACATTAGTGA TCTGGGTCTA GAACTAACCT TTTCTGTTGA AACTGATGTG
    TTTGGAGCAA TGGAAGAGGT GCCTTTGAAA CCTGGGGGTG GAAGTATTCT TGTGACACAA
    AATAACAAAG CGGAGTATGT CCAGCTTGTT ACTGAACTTC GAATGACAAG AGCCATTCAA
    CCTCAGATCA ATGCTTTTTT ACAGGGCTTT CATATGTTCA TTCCACCTTC CCTCATACAA
    CTTTTTGATG AATATGAATT GGAGCTACTG CTTTCTGGCA TGCCAGAAAT TGATGTGAGC
    GATTGGATAA GAAATACAGA ATACACAAGT GGCTATGAAA GAGAAGATCC AGTTATTCAG
    TGGTTCTGGG AAGTTGTGGA AGGCATTACT CCAGAGGAGA GAGTTCTTCT CTTGCAGTTT
    GTTACTGGCA GTTCCAGGGT CCCACATGGT GGATTTGCTA ACATCATGGG TGGAAGTGGA
    TTGCAAAACT TTACAATTGC TGCTGTGCCA TATACTCCAA ATCTCCTACC AACTTCAAGC
    ACATGCATCA ACATGCTCAA GTTACCTGAA TACCCAAGTA AAGAAATACT CAAGGACAGA
    CTTCTTGTGG CGTTACATTG TGGCAGCTAT GGTTACACAA TGGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe292659
    Clone ID Related Accession (Same CDS sequence) XM_022426401.1 , XM_022426402.1
    Accession Version XM_022426401.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2391bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase HACE1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGAGTAAAT TATTAGAATA CAGTGCTGAT GTCAACATTT GTAATAATGA AGGCCTTACA 
    GCAATACACT GGCTCGCTGT GAATGGGCGG ACAGAACTAC TCCATGACCT TGTGCAGCAC
    GTCAGTGACG TCGACGTTGA GGATGCGATG GGGCAGACAG CACTGCATGT CGCCTGCCAG
    AATGGTCACA AGACGACAGT GCAGTGCTTG CTAGACAGTG GTGCTGATAT TAACAGGCCA
    AATGTATCTG GAGCCACTCC ATTGTACTTT GCTTGCAGTC ATGGTCAGAG AGACACAGCA
    CAAATTCTTC TGTTACGAGG AGCTAAATAT CTGTCAGATA AAAATGGAGT AACGCCTCTG
    GATTTATGTG TACAGGGTGG CTATGGAGAG ACTTGTGAAG TATTAATTCA GTATCACCCT
    AGGCTTTTCC AGACAATTAT TCAAATGACA CAGAATGAAG ATCTCCGAGA AAACATGTTA
    CGGCAAGTTC TGGAACATTT GTCTCAGCAA AGTGAAAGCC AGTACCTAAA GATTCTAACA
    AGCCTTGCTG AAGTTGCCAC AACAAATGGG CATAAACTGC TTAGTCTGTC TAGTAATTAT
    GATGCTCAGA TGAAGAGCCT CTTAAGGATT GTGAGGATAT TTTGTCATGT CTTTCGAATT
    GGTCCATCCT CTCCCAGTAA TGGAATTGAT TTGGGCTACA ACGGGAATAA AACTCCAAGA
    AGCCAAGTGT TCAAGCCTCT TGAATTGCTT TGGCACTCAT TAGATGAATG GCTAGTTTTA
    ATAGCTACAG AATTGATGAA AAACAAAAAG GACTCAACGG ATATCACTTC TATTTTACTG
    AAACAAAAAG GCCAAGATCA AGATGGTACT TCCATTCCTC CATTTGAACC TCCAGGACCT
    GGGAGCTATG AAAATCTATC CAGTGGCACA GGAGAATCTA AACCAGATGC TCTTGGAGGG
    AAACAGGAAG CCAGTGCAGA CTGTCAGGAT GTTATTTCTA TGACAGCTAA CCGGCTAAGT
    GCTGTCATTC AAGCTTTTTA CATGTGCTGT TCTTGTCAGA TGCCTCCAGG AATGACCTCA
    CCCCGTTTCA TTGAATTTGT CTGCAAACAT GATGAAGTTT TAAAATGCTT TGTTAATAGA
    AATCCCAAAA TTATATTTGA TCACTTTCAT TTCCTCCTTG AATGTCCTGA GTTGATGTCA
    AGATTCATGC ATATCATAAA AGCACAGCCA TTTAAAGATC GCTGTGAGTG GTTCTATGAA
    CATTTGCACT CAGGACAACC AGATTCTGAC ATGGTGCACA GGCCAGTGAA TGAAAATGAT
    ATCCTGCTTG TTCACAGAGA TTCTATTTTT AGGAGTAGCT GTGAAGTTGT GTCAAAAGCA
    AACTGTGCAA AGCTAAAGCA AGGAATTGCT GTACGGTTCC ATGGAGAAGA AGGCATGGGC
    CAAGGTGTTG TGCGTGAGTG GTTTGATATT CTGTCTAATG AGATAGTGAA TCCTGATTAT
    GCATTGTTCA CCCAGTCAGC TGATGGAACA ACTTTTCAGC CTAATAGTAA TTCCTATGTA
    AATCCTGATC ACTTGAACTA TTTCCGGTTT GCTGGACAGA TCTTGGGATT AGCTTTGAAC
    CACAGGCAGC TGGTCAACAT TTACTTCACA AGATCGTTCT ATAAGCACAT TCTTGGTATC
    CCTGTAAATT ACCAAGATGT GGCATCCATT GATCCAGAAT ATGCCAAAAA TTTGCAGTGG
    ATTTTAGATA ATGACATTAG TGATCTGGGT CTAGAACTAA CCTTTTCTGT TGAAACTGAT
    GTGTTTGGAG CAATGGAAGA GGTGCCTTTG AAACCTGGGG GTGGAAGTAT TCTTGTGACA
    CAAAATAACA AAGCGGAGTA TGTCCAGCTT GTTACTGAAC TTCGAATGAC AAGAGCCATT
    CAACCTCAGA TCAATGCTTT TTTACAGGGC TTTCATATGT TCATTCCACC TTCCCTCATA
    CAACTTTTTG ATGAATATGA ATTGGAGCTA CTGCTTTCTG GCATGCCAGA AATTGATGTG
    AGCGATTGGA TAAGAAATAC AGAATACACA AGTGGCTATG AAAGAGAAGA TCCAGTTATT
    CAGTGGTTCT GGGAAGTTGT GGAAGGCATT ACTCCAGAGG AGAGAGTTCT TCTCTTGCAG
    TTTGTTACTG GCAGTTCCAG GGTCCCACAT GGTGGATTTG CTAACATCAT GGGTGGAAGT
    GGATTGCAAA ACTTTACAAT TGCTGCTGTG CCATATACTC CAAATCTCCT ACCAACTTCA
    AGCACATGCA TCAACATGCT CAAGTTACCT GAATACCCAA GTAAAGAAAT ACTCAAGGAC
    AGACTTCTTG TGGCGTTACA TTGTGGCAGC TATGGTTACA CAATGGCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022282109.1
    CDS231..2621
    Translation

    Target ORF information:

    RefSeq Version XM_022426401.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426401.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGAGTAAAT TATTAGAATA CAGTGCTGAT GTCAACATTT GTAATAATGA AGGCCTTACA 
    GCAATACACT GGCTCGCTGT GAATGGGCGG ACAGAACTAC TCCATGACCT TGTGCAGCAC
    GTCAGTGACG TCGACGTTGA GGATGCGATG GGGCAGACAG CACTGCATGT CGCCTGCCAG
    AATGGTCACA AGACGACAGT GCAGTGCTTG CTAGACAGTG GTGCTGATAT TAACAGGCCA
    AATGTATCTG GAGCCACTCC ATTGTACTTT GCTTGCAGTC ATGGTCAGAG AGACACAGCA
    CAAATTCTTC TGTTACGAGG AGCTAAATAT CTGTCAGATA AAAATGGAGT AACGCCTCTG
    GATTTATGTG TACAGGGTGG CTATGGAGAG ACTTGTGAAG TATTAATTCA GTATCACCCT
    AGGCTTTTCC AGACAATTAT TCAAATGACA CAGAATGAAG ATCTCCGAGA AAACATGTTA
    CGGCAAGTTC TGGAACATTT GTCTCAGCAA AGTGAAAGCC AGTACCTAAA GATTCTAACA
    AGCCTTGCTG AAGTTGCCAC AACAAATGGG CATAAACTGC TTAGTCTGTC TAGTAATTAT
    GATGCTCAGA TGAAGAGCCT CTTAAGGATT GTGAGGATAT TTTGTCATGT CTTTCGAATT
    GGTCCATCCT CTCCCAGTAA TGGAATTGAT TTGGGCTACA ACGGGAATAA AACTCCAAGA
    AGCCAAGTGT TCAAGCCTCT TGAATTGCTT TGGCACTCAT TAGATGAATG GCTAGTTTTA
    ATAGCTACAG AATTGATGAA AAACAAAAAG GACTCAACGG ATATCACTTC TATTTTACTG
    AAACAAAAAG GCCAAGATCA AGATGGTACT TCCATTCCTC CATTTGAACC TCCAGGACCT
    GGGAGCTATG AAAATCTATC CAGTGGCACA GGAGAATCTA AACCAGATGC TCTTGGAGGG
    AAACAGGAAG CCAGTGCAGA CTGTCAGGAT GTTATTTCTA TGACAGCTAA CCGGCTAAGT
    GCTGTCATTC AAGCTTTTTA CATGTGCTGT TCTTGTCAGA TGCCTCCAGG AATGACCTCA
    CCCCGTTTCA TTGAATTTGT CTGCAAACAT GATGAAGTTT TAAAATGCTT TGTTAATAGA
    AATCCCAAAA TTATATTTGA TCACTTTCAT TTCCTCCTTG AATGTCCTGA GTTGATGTCA
    AGATTCATGC ATATCATAAA AGCACAGCCA TTTAAAGATC GCTGTGAGTG GTTCTATGAA
    CATTTGCACT CAGGACAACC AGATTCTGAC ATGGTGCACA GGCCAGTGAA TGAAAATGAT
    ATCCTGCTTG TTCACAGAGA TTCTATTTTT AGGAGTAGCT GTGAAGTTGT GTCAAAAGCA
    AACTGTGCAA AGCTAAAGCA AGGAATTGCT GTACGGTTCC ATGGAGAAGA AGGCATGGGC
    CAAGGTGTTG TGCGTGAGTG GTTTGATATT CTGTCTAATG AGATAGTGAA TCCTGATTAT
    GCATTGTTCA CCCAGTCAGC TGATGGAACA ACTTTTCAGC CTAATAGTAA TTCCTATGTA
    AATCCTGATC ACTTGAACTA TTTCCGGTTT GCTGGACAGA TCTTGGGATT AGCTTTGAAC
    CACAGGCAGC TGGTCAACAT TTACTTCACA AGATCGTTCT ATAAGCACAT TCTTGGTATC
    CCTGTAAATT ACCAAGATGT GGCATCCATT GATCCAGAAT ATGCCAAAAA TTTGCAGTGG
    ATTTTAGATA ATGACATTAG TGATCTGGGT CTAGAACTAA CCTTTTCTGT TGAAACTGAT
    GTGTTTGGAG CAATGGAAGA GGTGCCTTTG AAACCTGGGG GTGGAAGTAT TCTTGTGACA
    CAAAATAACA AAGCGGAGTA TGTCCAGCTT GTTACTGAAC TTCGAATGAC AAGAGCCATT
    CAACCTCAGA TCAATGCTTT TTTACAGGGC TTTCATATGT TCATTCCACC TTCCCTCATA
    CAACTTTTTG ATGAATATGA ATTGGAGCTA CTGCTTTCTG GCATGCCAGA AATTGATGTG
    AGCGATTGGA TAAGAAATAC AGAATACACA AGTGGCTATG AAAGAGAAGA TCCAGTTATT
    CAGTGGTTCT GGGAAGTTGT GGAAGGCATT ACTCCAGAGG AGAGAGTTCT TCTCTTGCAG
    TTTGTTACTG GCAGTTCCAG GGTCCCACAT GGTGGATTTG CTAACATCAT GGGTGGAAGT
    GGATTGCAAA ACTTTACAAT TGCTGCTGTG CCATATACTC CAAATCTCCT ACCAACTTCA
    AGCACATGCA TCAACATGCT CAAGTTACCT GAATACCCAA GTAAAGAAAT ACTCAAGGAC
    AGACTTCTTG TGGCGTTACA TTGTGGCAGC TATGGTTACA CAATGGCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe292659
    Clone ID Related Accession (Same CDS sequence) XM_022426401.1 , XM_022426402.1
    Accession Version XM_022426402.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2391bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase HACE1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGAGTAAAT TATTAGAATA CAGTGCTGAT GTCAACATTT GTAATAATGA AGGCCTTACA 
    GCAATACACT GGCTCGCTGT GAATGGGCGG ACAGAACTAC TCCATGACCT TGTGCAGCAC
    GTCAGTGACG TCGACGTTGA GGATGCGATG GGGCAGACAG CACTGCATGT CGCCTGCCAG
    AATGGTCACA AGACGACAGT GCAGTGCTTG CTAGACAGTG GTGCTGATAT TAACAGGCCA
    AATGTATCTG GAGCCACTCC ATTGTACTTT GCTTGCAGTC ATGGTCAGAG AGACACAGCA
    CAAATTCTTC TGTTACGAGG AGCTAAATAT CTGTCAGATA AAAATGGAGT AACGCCTCTG
    GATTTATGTG TACAGGGTGG CTATGGAGAG ACTTGTGAAG TATTAATTCA GTATCACCCT
    AGGCTTTTCC AGACAATTAT TCAAATGACA CAGAATGAAG ATCTCCGAGA AAACATGTTA
    CGGCAAGTTC TGGAACATTT GTCTCAGCAA AGTGAAAGCC AGTACCTAAA GATTCTAACA
    AGCCTTGCTG AAGTTGCCAC AACAAATGGG CATAAACTGC TTAGTCTGTC TAGTAATTAT
    GATGCTCAGA TGAAGAGCCT CTTAAGGATT GTGAGGATAT TTTGTCATGT CTTTCGAATT
    GGTCCATCCT CTCCCAGTAA TGGAATTGAT TTGGGCTACA ACGGGAATAA AACTCCAAGA
    AGCCAAGTGT TCAAGCCTCT TGAATTGCTT TGGCACTCAT TAGATGAATG GCTAGTTTTA
    ATAGCTACAG AATTGATGAA AAACAAAAAG GACTCAACGG ATATCACTTC TATTTTACTG
    AAACAAAAAG GCCAAGATCA AGATGGTACT TCCATTCCTC CATTTGAACC TCCAGGACCT
    GGGAGCTATG AAAATCTATC CAGTGGCACA GGAGAATCTA AACCAGATGC TCTTGGAGGG
    AAACAGGAAG CCAGTGCAGA CTGTCAGGAT GTTATTTCTA TGACAGCTAA CCGGCTAAGT
    GCTGTCATTC AAGCTTTTTA CATGTGCTGT TCTTGTCAGA TGCCTCCAGG AATGACCTCA
    CCCCGTTTCA TTGAATTTGT CTGCAAACAT GATGAAGTTT TAAAATGCTT TGTTAATAGA
    AATCCCAAAA TTATATTTGA TCACTTTCAT TTCCTCCTTG AATGTCCTGA GTTGATGTCA
    AGATTCATGC ATATCATAAA AGCACAGCCA TTTAAAGATC GCTGTGAGTG GTTCTATGAA
    CATTTGCACT CAGGACAACC AGATTCTGAC ATGGTGCACA GGCCAGTGAA TGAAAATGAT
    ATCCTGCTTG TTCACAGAGA TTCTATTTTT AGGAGTAGCT GTGAAGTTGT GTCAAAAGCA
    AACTGTGCAA AGCTAAAGCA AGGAATTGCT GTACGGTTCC ATGGAGAAGA AGGCATGGGC
    CAAGGTGTTG TGCGTGAGTG GTTTGATATT CTGTCTAATG AGATAGTGAA TCCTGATTAT
    GCATTGTTCA CCCAGTCAGC TGATGGAACA ACTTTTCAGC CTAATAGTAA TTCCTATGTA
    AATCCTGATC ACTTGAACTA TTTCCGGTTT GCTGGACAGA TCTTGGGATT AGCTTTGAAC
    CACAGGCAGC TGGTCAACAT TTACTTCACA AGATCGTTCT ATAAGCACAT TCTTGGTATC
    CCTGTAAATT ACCAAGATGT GGCATCCATT GATCCAGAAT ATGCCAAAAA TTTGCAGTGG
    ATTTTAGATA ATGACATTAG TGATCTGGGT CTAGAACTAA CCTTTTCTGT TGAAACTGAT
    GTGTTTGGAG CAATGGAAGA GGTGCCTTTG AAACCTGGGG GTGGAAGTAT TCTTGTGACA
    CAAAATAACA AAGCGGAGTA TGTCCAGCTT GTTACTGAAC TTCGAATGAC AAGAGCCATT
    CAACCTCAGA TCAATGCTTT TTTACAGGGC TTTCATATGT TCATTCCACC TTCCCTCATA
    CAACTTTTTG ATGAATATGA ATTGGAGCTA CTGCTTTCTG GCATGCCAGA AATTGATGTG
    AGCGATTGGA TAAGAAATAC AGAATACACA AGTGGCTATG AAAGAGAAGA TCCAGTTATT
    CAGTGGTTCT GGGAAGTTGT GGAAGGCATT ACTCCAGAGG AGAGAGTTCT TCTCTTGCAG
    TTTGTTACTG GCAGTTCCAG GGTCCCACAT GGTGGATTTG CTAACATCAT GGGTGGAAGT
    GGATTGCAAA ACTTTACAAT TGCTGCTGTG CCATATACTC CAAATCTCCT ACCAACTTCA
    AGCACATGCA TCAACATGCT CAAGTTACCT GAATACCCAA GTAAAGAAAT ACTCAAGGAC
    AGACTTCTTG TGGCGTTACA TTGTGGCAGC TATGGTTACA CAATGGCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022282110.1
    CDS138..2528
    Translation

    Target ORF information:

    RefSeq Version XM_022426402.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426402.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGAGTAAAT TATTAGAATA CAGTGCTGAT GTCAACATTT GTAATAATGA AGGCCTTACA 
    GCAATACACT GGCTCGCTGT GAATGGGCGG ACAGAACTAC TCCATGACCT TGTGCAGCAC
    GTCAGTGACG TCGACGTTGA GGATGCGATG GGGCAGACAG CACTGCATGT CGCCTGCCAG
    AATGGTCACA AGACGACAGT GCAGTGCTTG CTAGACAGTG GTGCTGATAT TAACAGGCCA
    AATGTATCTG GAGCCACTCC ATTGTACTTT GCTTGCAGTC ATGGTCAGAG AGACACAGCA
    CAAATTCTTC TGTTACGAGG AGCTAAATAT CTGTCAGATA AAAATGGAGT AACGCCTCTG
    GATTTATGTG TACAGGGTGG CTATGGAGAG ACTTGTGAAG TATTAATTCA GTATCACCCT
    AGGCTTTTCC AGACAATTAT TCAAATGACA CAGAATGAAG ATCTCCGAGA AAACATGTTA
    CGGCAAGTTC TGGAACATTT GTCTCAGCAA AGTGAAAGCC AGTACCTAAA GATTCTAACA
    AGCCTTGCTG AAGTTGCCAC AACAAATGGG CATAAACTGC TTAGTCTGTC TAGTAATTAT
    GATGCTCAGA TGAAGAGCCT CTTAAGGATT GTGAGGATAT TTTGTCATGT CTTTCGAATT
    GGTCCATCCT CTCCCAGTAA TGGAATTGAT TTGGGCTACA ACGGGAATAA AACTCCAAGA
    AGCCAAGTGT TCAAGCCTCT TGAATTGCTT TGGCACTCAT TAGATGAATG GCTAGTTTTA
    ATAGCTACAG AATTGATGAA AAACAAAAAG GACTCAACGG ATATCACTTC TATTTTACTG
    AAACAAAAAG GCCAAGATCA AGATGGTACT TCCATTCCTC CATTTGAACC TCCAGGACCT
    GGGAGCTATG AAAATCTATC CAGTGGCACA GGAGAATCTA AACCAGATGC TCTTGGAGGG
    AAACAGGAAG CCAGTGCAGA CTGTCAGGAT GTTATTTCTA TGACAGCTAA CCGGCTAAGT
    GCTGTCATTC AAGCTTTTTA CATGTGCTGT TCTTGTCAGA TGCCTCCAGG AATGACCTCA
    CCCCGTTTCA TTGAATTTGT CTGCAAACAT GATGAAGTTT TAAAATGCTT TGTTAATAGA
    AATCCCAAAA TTATATTTGA TCACTTTCAT TTCCTCCTTG AATGTCCTGA GTTGATGTCA
    AGATTCATGC ATATCATAAA AGCACAGCCA TTTAAAGATC GCTGTGAGTG GTTCTATGAA
    CATTTGCACT CAGGACAACC AGATTCTGAC ATGGTGCACA GGCCAGTGAA TGAAAATGAT
    ATCCTGCTTG TTCACAGAGA TTCTATTTTT AGGAGTAGCT GTGAAGTTGT GTCAAAAGCA
    AACTGTGCAA AGCTAAAGCA AGGAATTGCT GTACGGTTCC ATGGAGAAGA AGGCATGGGC
    CAAGGTGTTG TGCGTGAGTG GTTTGATATT CTGTCTAATG AGATAGTGAA TCCTGATTAT
    GCATTGTTCA CCCAGTCAGC TGATGGAACA ACTTTTCAGC CTAATAGTAA TTCCTATGTA
    AATCCTGATC ACTTGAACTA TTTCCGGTTT GCTGGACAGA TCTTGGGATT AGCTTTGAAC
    CACAGGCAGC TGGTCAACAT TTACTTCACA AGATCGTTCT ATAAGCACAT TCTTGGTATC
    CCTGTAAATT ACCAAGATGT GGCATCCATT GATCCAGAAT ATGCCAAAAA TTTGCAGTGG
    ATTTTAGATA ATGACATTAG TGATCTGGGT CTAGAACTAA CCTTTTCTGT TGAAACTGAT
    GTGTTTGGAG CAATGGAAGA GGTGCCTTTG AAACCTGGGG GTGGAAGTAT TCTTGTGACA
    CAAAATAACA AAGCGGAGTA TGTCCAGCTT GTTACTGAAC TTCGAATGAC AAGAGCCATT
    CAACCTCAGA TCAATGCTTT TTTACAGGGC TTTCATATGT TCATTCCACC TTCCCTCATA
    CAACTTTTTG ATGAATATGA ATTGGAGCTA CTGCTTTCTG GCATGCCAGA AATTGATGTG
    AGCGATTGGA TAAGAAATAC AGAATACACA AGTGGCTATG AAAGAGAAGA TCCAGTTATT
    CAGTGGTTCT GGGAAGTTGT GGAAGGCATT ACTCCAGAGG AGAGAGTTCT TCTCTTGCAG
    TTTGTTACTG GCAGTTCCAG GGTCCCACAT GGTGGATTTG CTAACATCAT GGGTGGAAGT
    GGATTGCAAA ACTTTACAAT TGCTGCTGTG CCATATACTC CAAATCTCCT ACCAACTTCA
    AGCACATGCA TCAACATGCT CAAGTTACCT GAATACCCAA GTAAAGAAAT ACTCAAGGAC
    AGACTTCTTG TGGCGTTACA TTGTGGCAGC TATGGTTACA CAATGGCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe292660
    Clone ID Related Accession (Same CDS sequence) XM_022426403.1
    Accession Version XM_022426403.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2226bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase HACE1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCGGAC AGAACTACTC CATGACCTTG TGCAGCACGT CAGTGACGTC GACGTTGAGG 
    ATGCGATGGG GCAGACAGCA CTGCATGTCG CCTGCCAGAA TGGTCACAAG ACGTCATGGT
    CAGAGAGACA CAGCACAAAT TCTTCTGTTA CGAGGAGCTA AATATCTGTC AGATAAAAAT
    GGAGTAACGC CTCTGGATTT ATGTGTACAG GGTGGCTATG GAGAGACTTG TGAAGTATTA
    ATTCAGTATC ACCCTAGGCT TTTCCAGACA ATTATTCAAA TGACACAGAA TGAAGATCTC
    CGAGAAAACA TGTTACGGCA AGTTCTGGAA CATTTGTCTC AGCAAAGTGA AAGCCAGTAC
    CTAAAGATTC TAACAAGCCT TGCTGAAGTT GCCACAACAA ATGGGCATAA ACTGCTTAGT
    CTGTCTAGTA ATTATGATGC TCAGATGAAG AGCCTCTTAA GGATTGTGAG GATATTTTGT
    CATGTCTTTC GAATTGGTCC ATCCTCTCCC AGTAATGGAA TTGATTTGGG CTACAACGGG
    AATAAAACTC CAAGAAGCCA AGTGTTCAAG CCTCTTGAAT TGCTTTGGCA CTCATTAGAT
    GAATGGCTAG TTTTAATAGC TACAGAATTG ATGAAAAACA AAAAGGACTC AACGGATATC
    ACTTCTATTT TACTGAAACA AAAAGGCCAA GATCAAGATG GTACTTCCAT TCCTCCATTT
    GAACCTCCAG GACCTGGGAG CTATGAAAAT CTATCCAGTG GCACAGGAGA ATCTAAACCA
    GATGCTCTTG GAGGGAAACA GGAAGCCAGT GCAGACTGTC AGGATGTTAT TTCTATGACA
    GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT
    CCAGGAATGA CCTCACCCCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA
    TGCTTTGTTA ATAGAAATCC CAAAATTATA TTTGATCACT TTCATTTCCT CCTTGAATGT
    CCTGAGTTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCATTTAA AGATCGCTGT
    GAGTGGTTCT ATGAACATTT GCACTCAGGA CAACCAGATT CTGACATGGT GCACAGGCCA
    GTGAATGAAA ATGATATCCT GCTTGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA
    GTTGTGTCAA AAGCAAACTG TGCAAAGCTA AAGCAAGGAA TTGCTGTACG GTTCCATGGA
    GAAGAAGGCA TGGGCCAAGG TGTTGTGCGT GAGTGGTTTG ATATTCTGTC TAATGAGATA
    GTGAATCCTG ATTATGCATT GTTCACCCAG TCAGCTGATG GAACAACTTT TCAGCCTAAT
    AGTAATTCCT ATGTAAATCC TGATCACTTG AACTATTTCC GGTTTGCTGG ACAGATCTTG
    GGATTAGCTT TGAACCACAG GCAGCTGGTC AACATTTACT TCACAAGATC GTTCTATAAG
    CACATTCTTG GTATCCCTGT AAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCC
    AAAAATTTGC AGTGGATTTT AGATAATGAC ATTAGTGATC TGGGTCTAGA ACTAACCTTT
    TCTGTTGAAA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CTTTGAAACC TGGGGGTGGA
    AGTATTCTTG TGACACAAAA TAACAAAGCG GAGTATGTCC AGCTTGTTAC TGAACTTCGA
    ATGACAAGAG CCATTCAACC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT
    CCACCTTCCC TCATACAACT TTTTGATGAA TATGAATTGG AGCTACTGCT TTCTGGCATG
    CCAGAAATTG ATGTGAGCGA TTGGATAAGA AATACAGAAT ACACAAGTGG CTATGAAAGA
    GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTGGAAG GCATTACTCC AGAGGAGAGA
    GTTCTTCTCT TGCAGTTTGT TACTGGCAGT TCCAGGGTCC CACATGGTGG ATTTGCTAAC
    ATCATGGGTG GAAGTGGATT GCAAAACTTT ACAATTGCTG CTGTGCCATA TACTCCAAAT
    CTCCTACCAA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA
    GAAATACTCA AGGACAGACT TCTTGTGGCG TTACATTGTG GCAGCTATGG TTACACAATG
    GCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022282111.1
    CDS1..2226
    Translation

    Target ORF information:

    RefSeq Version XM_022426403.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426403.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGCGGAC AGAACTACTC CATGACCTTG TGCAGCACGT CAGTGACGTC GACGTTGAGG 
    ATGCGATGGG GCAGACAGCA CTGCATGTCG CCTGCCAGAA TGGTCACAAG ACGTCATGGT
    CAGAGAGACA CAGCACAAAT TCTTCTGTTA CGAGGAGCTA AATATCTGTC AGATAAAAAT
    GGAGTAACGC CTCTGGATTT ATGTGTACAG GGTGGCTATG GAGAGACTTG TGAAGTATTA
    ATTCAGTATC ACCCTAGGCT TTTCCAGACA ATTATTCAAA TGACACAGAA TGAAGATCTC
    CGAGAAAACA TGTTACGGCA AGTTCTGGAA CATTTGTCTC AGCAAAGTGA AAGCCAGTAC
    CTAAAGATTC TAACAAGCCT TGCTGAAGTT GCCACAACAA ATGGGCATAA ACTGCTTAGT
    CTGTCTAGTA ATTATGATGC TCAGATGAAG AGCCTCTTAA GGATTGTGAG GATATTTTGT
    CATGTCTTTC GAATTGGTCC ATCCTCTCCC AGTAATGGAA TTGATTTGGG CTACAACGGG
    AATAAAACTC CAAGAAGCCA AGTGTTCAAG CCTCTTGAAT TGCTTTGGCA CTCATTAGAT
    GAATGGCTAG TTTTAATAGC TACAGAATTG ATGAAAAACA AAAAGGACTC AACGGATATC
    ACTTCTATTT TACTGAAACA AAAAGGCCAA GATCAAGATG GTACTTCCAT TCCTCCATTT
    GAACCTCCAG GACCTGGGAG CTATGAAAAT CTATCCAGTG GCACAGGAGA ATCTAAACCA
    GATGCTCTTG GAGGGAAACA GGAAGCCAGT GCAGACTGTC AGGATGTTAT TTCTATGACA
    GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT
    CCAGGAATGA CCTCACCCCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA
    TGCTTTGTTA ATAGAAATCC CAAAATTATA TTTGATCACT TTCATTTCCT CCTTGAATGT
    CCTGAGTTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCATTTAA AGATCGCTGT
    GAGTGGTTCT ATGAACATTT GCACTCAGGA CAACCAGATT CTGACATGGT GCACAGGCCA
    GTGAATGAAA ATGATATCCT GCTTGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA
    GTTGTGTCAA AAGCAAACTG TGCAAAGCTA AAGCAAGGAA TTGCTGTACG GTTCCATGGA
    GAAGAAGGCA TGGGCCAAGG TGTTGTGCGT GAGTGGTTTG ATATTCTGTC TAATGAGATA
    GTGAATCCTG ATTATGCATT GTTCACCCAG TCAGCTGATG GAACAACTTT TCAGCCTAAT
    AGTAATTCCT ATGTAAATCC TGATCACTTG AACTATTTCC GGTTTGCTGG ACAGATCTTG
    GGATTAGCTT TGAACCACAG GCAGCTGGTC AACATTTACT TCACAAGATC GTTCTATAAG
    CACATTCTTG GTATCCCTGT AAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCC
    AAAAATTTGC AGTGGATTTT AGATAATGAC ATTAGTGATC TGGGTCTAGA ACTAACCTTT
    TCTGTTGAAA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CTTTGAAACC TGGGGGTGGA
    AGTATTCTTG TGACACAAAA TAACAAAGCG GAGTATGTCC AGCTTGTTAC TGAACTTCGA
    ATGACAAGAG CCATTCAACC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT
    CCACCTTCCC TCATACAACT TTTTGATGAA TATGAATTGG AGCTACTGCT TTCTGGCATG
    CCAGAAATTG ATGTGAGCGA TTGGATAAGA AATACAGAAT ACACAAGTGG CTATGAAAGA
    GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTGGAAG GCATTACTCC AGAGGAGAGA
    GTTCTTCTCT TGCAGTTTGT TACTGGCAGT TCCAGGGTCC CACATGGTGG ATTTGCTAAC
    ATCATGGGTG GAAGTGGATT GCAAAACTTT ACAATTGCTG CTGTGCCATA TACTCCAAAT
    CTCCTACCAA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA
    GAAATACTCA AGGACAGACT TCTTGTGGCG TTACATTGTG GCAGCTATGG TTACACAATG
    GCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.