HACE1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	HACE1
Entrez Gene ID	475010
Full Name	HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1
General protein information	Preferred Names HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 Names E3 ubiquitin-protein ligase HACE1
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_849139.4	XP_854232.1	E3 ubiquitin-protein ligase HACE1 isoform X1
XM_022426401.1	XP_022282109.1	E3 ubiquitin-protein ligase HACE1 isoform X3
XM_022426402.1	XP_022282110.1	E3 ubiquitin-protein ligase HACE1 isoform X3
XM_022426403.1	XP_022282111.1	E3 ubiquitin-protein ligase HACE1 isoform X5
XM_022426399.1	XP_022282107.1	E3 ubiquitin-protein ligase HACE1 isoform X2
XM_005627667.2	XP_005627724.1	E3 ubiquitin-protein ligase HACE1 isoform X2
XM_849139.5	XP_854232.1	E3 ubiquitin-protein ligase HACE1 isoform X1
XM_005627667.3	XP_005627724.1	E3 ubiquitin-protein ligase HACE1 isoform X4

Pathways from BioSystems

Homologies

Homo sapiens (human)	HACE1	NP_065822.2
Canis lupus familiaris (dog)	HACE1	XP_854232.1
Pan troglodytes (chimpanzee)	HACE1	XP_518654.2
Rattus norvegicus (Norway rat)	Hace1	NP_001102009.2
Gallus gallus (chicken)	HACE1	NP_001265084.1
Xenopus tropicalis (tropical clawed frog)	hace1	NP_001039269.1
Bos taurus (cattle)	HACE1	NP_001179574.2
Mus musculus (house mouse)	Hace1	NP_766061.2
Danio rerio (zebrafish)	hace1	XP_701235.5

Gene Ontology

The following HACE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HACE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe15637	XM_849139.5 Latest version!	Canis lupus familiaris HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe15638	XM_005627667.3 Latest version!	Canis lupus familiaris HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe292658	XM_022426399.1 Latest version!	Canis lupus familiaris HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OCe292659	XM_022426401.1 Latest version!	Canis lupus familiaris HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCe292659	XM_022426402.1 Latest version!	Canis lupus familiaris HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCe292660	XM_022426403.1 Latest version!	Canis lupus familiaris HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
OCe15637	XM_849139.4	Canis lupus familiaris HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe15638	XM_005627667.2	Canis lupus familiaris HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HACE1 ( XM_849139.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_854232.1 Canis lupus familiaris HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe15637
Clone ID Related Accession (Same CDS sequence)	XM_849139.4 , XM_849139.5
Accession Version	XM_849139.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase HACE1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_849139.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAG CGATGGAGCA GCTCAACCGC CTCACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGCTGC CGGACGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT 
GATCAACACA GGTCTGTTTC TGAGCTACTA TCAAATTCAA AGTTTGATGT CAATTATGCT 
TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC 
TTGGTCCTGC TATTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC AGGATGTACA 
CCCCTTCATT TGGCAGCTAG AAATGGGCAG AAGAAATGCA TGAGTAAATT ATTAGAATAC 
AGTGCTGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAATACACTG GCTCGCTGTG 
AATGGGCGGA CAGAACTACT CCATGACCTT GTGCAGCACG TCAGTGACGT CGACGTTGAG 
GATGCGATGG GGCAGACAGC ACTGCATGTC GCCTGCCAGA ATGGTCACAA GACGACAGTG 
CAGTGCTTGC TAGACAGTGG TGCTGATATT AACAGGCCAA ATGTATCTGG AGCCACTCCA 
TTGTACTTTG CTTGCAGTCA TGGTCAGAGA GACACAGCAC AAATTCTTCT GTTACGAGGA 
GCTAAATATC TGTCAGATAA AAATGGAGTA ACGCCTCTGG ATTTATGTGT ACAGGGTGGC 
TATGGAGAGA CTTGTGAAGT ATTAATTCAG TATCACCCTA GGCTTTTCCA GACAATTATT 
CAAATGACAC AGAATGAAGA TCTCCGAGAA AACATGTTAC GGCAAGTTCT GGAACATTTG 
TCTCAGCAAA GTGAAAGCCA GTACCTAAAG ATTCTAACAA GCCTTGCTGA AGTTGCCACA 
ACAAATGGGC ATAAACTGCT TAGTCTGTCT AGTAATTATG ATGCTCAGAT GAAGAGCCTC 
TTAAGGATTG TGAGGATATT TTGTCATGTC TTTCGAATTG GTCCATCCTC TCCCAGTAAT 
GGAATTGATT TGGGCTACAA CGGGAATAAA ACTCCAAGAA GCCAAGTGTT CAAGCCTCTT 
GAATTGCTTT GGCACTCATT AGATGAATGG CTAGTTTTAA TAGCTACAGA ATTGATGAAA 
AACAAAAAGG ACTCAACGGA TATCACTTCT ATTTTACTGA AACAAAAAGG CCAAGATCAA 
GATGGTACTT CCATTCCTCC ATTTGAACCT CCAGGACCTG GGAGCTATGA AAATCTATCC 
AGTGGCACAG GAGAATCTAA ACCAGATGCT CTTGGAGGGA AACAGGAAGC CAGTGCAGAC 
TGTCAGGATG TTATTTCTAT GACAGCTAAC CGGCTAAGTG CTGTCATTCA AGCTTTTTAC 
ATGTGCTGTT CTTGTCAGAT GCCTCCAGGA ATGACCTCAC CCCGTTTCAT TGAATTTGTC 
TGCAAACATG ATGAAGTTTT AAAATGCTTT GTTAATAGAA ATCCCAAAAT TATATTTGAT 
CACTTTCATT TCCTCCTTGA ATGTCCTGAG TTGATGTCAA GATTCATGCA TATCATAAAA 
GCACAGCCAT TTAAAGATCG CTGTGAGTGG TTCTATGAAC ATTTGCACTC AGGACAACCA 
GATTCTGACA TGGTGCACAG GCCAGTGAAT GAAAATGATA TCCTGCTTGT TCACAGAGAT 
TCTATTTTTA GGAGTAGCTG TGAAGTTGTG TCAAAAGCAA ACTGTGCAAA GCTAAAGCAA 
GGAATTGCTG TACGGTTCCA TGGAGAAGAA GGCATGGGCC AAGGTGTTGT GCGTGAGTGG 
TTTGATATTC TGTCTAATGA GATAGTGAAT CCTGATTATG CATTGTTCAC CCAGTCAGCT 
GATGGAACAA CTTTTCAGCC TAATAGTAAT TCCTATGTAA ATCCTGATCA CTTGAACTAT 
TTCCGGTTTG CTGGACAGAT CTTGGGATTA GCTTTGAACC ACAGGCAGCT GGTCAACATT 
TACTTCACAA GATCGTTCTA TAAGCACATT CTTGGTATCC CTGTAAATTA CCAAGATGTG 
GCATCCATTG ATCCAGAATA TGCCAAAAAT TTGCAGTGGA TTTTAGATAA TGACATTAGT 
GATCTGGGTC TAGAACTAAC CTTTTCTGTT GAAACTGATG TGTTTGGAGC AATGGAAGAG 
GTGCCTTTGA AACCTGGGGG TGGAAGTATT CTTGTGACAC AAAATAACAA AGCGGAGTAT 
GTCCAGCTTG TTACTGAACT TCGAATGACA AGAGCCATTC AACCTCAGAT CAATGCTTTT 
TTACAGGGCT TTCATATGTT CATTCCACCT TCCCTCATAC AACTTTTTGA TGAATATGAA 
TTGGAGCTAC TGCTTTCTGG CATGCCAGAA ATTGATGTGA GCGATTGGAT AAGAAATACA 
GAATACACAA GTGGCTATGA AAGAGAAGAT CCAGTTATTC AGTGGTTCTG GGAAGTTGTG 
GAAGGCATTA CTCCAGAGGA GAGAGTTCTT CTCTTGCAGT TTGTTACTGG CAGTTCCAGG 
GTCCCACATG GTGGATTTGC TAACATCATG GGTGGAAGTG GATTGCAAAA CTTTACAATT 
GCTGCTGTGC CATATACTCC AAATCTCCTA CCAACTTCAA GCACATGCAT CAACATGCTC 
AAGTTACCTG AATACCCAAG TAAAGAAATA CTCAAGGACA GACTTCTTGT GGCGTTACAT 
TGTGGCAGCT ATGGTTACAC AATGGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_854232.1
CDS	190..2919
Translation	MERAMEQLNRLTRSLRRARTVELPDDNETAVYTLMPMVMADQHRSVSELLSNSKFDVNYAFGRVKRSLLHIAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTAIHWLAVNGRTELLHDLVQHVSDVDVEDAMGQTALHVACQNGHKTTVQCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLLRGAKYLSDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRIFCHVFRIGPSSPSNGIDLGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKKDSTDITSILLKQKGQDQDGTSIPPFEPPGPGSYENLSSGTGESKPDALGGKQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIRNTEYTSGYEREDPVIQWFWEVVEGITPEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	XM_849139.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_849139.4

ORF Insert Sequence:

ATGGAGAGAG CGATGGAGCA GCTCAACCGC CTCACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGCTGC CGGACGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT 
GATCAACACA GGTCTGTTTC TGAGCTACTA TCAAATTCAA AGTTTGATGT CAATTATGCT 
TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC 
TTGGTCCTGC TATTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC AGGATGTACA 
CCCCTTCATT TGGCAGCTAG AAATGGGCAG AAGAAATGCA TGAGTAAATT ATTAGAATAC 
AGTGCTGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAATACACTG GCTCGCTGTG 
AATGGGCGGA CAGAACTACT CCATGACCTT GTGCAGCACG TCAGTGACGT CGACGTTGAG 
GATGCGATGG GGCAGACAGC ACTGCATGTC GCCTGCCAGA ATGGTCACAA GACGACAGTG 
CAGTGCTTGC TAGACAGTGG TGCTGATATT AACAGGCCAA ATGTATCTGG AGCCACTCCA 
TTGTACTTTG CTTGCAGTCA TGGTCAGAGA GACACAGCAC AAATTCTTCT GTTACGAGGA 
GCTAAATATC TGTCAGATAA AAATGGAGTA ACGCCTCTGG ATTTATGTGT ACAGGGTGGC 
TATGGAGAGA CTTGTGAAGT ATTAATTCAG TATCACCCTA GGCTTTTCCA GACAATTATT 
CAAATGACAC AGAATGAAGA TCTCCGAGAA AACATGTTAC GGCAAGTTCT GGAACATTTG 
TCTCAGCAAA GTGAAAGCCA GTACCTAAAG ATTCTAACAA GCCTTGCTGA AGTTGCCACA 
ACAAATGGGC ATAAACTGCT TAGTCTGTCT AGTAATTATG ATGCTCAGAT GAAGAGCCTC 
TTAAGGATTG TGAGGATATT TTGTCATGTC TTTCGAATTG GTCCATCCTC TCCCAGTAAT 
GGAATTGATT TGGGCTACAA CGGGAATAAA ACTCCAAGAA GCCAAGTGTT CAAGCCTCTT 
GAATTGCTTT GGCACTCATT AGATGAATGG CTAGTTTTAA TAGCTACAGA ATTGATGAAA 
AACAAAAAGG ACTCAACGGA TATCACTTCT ATTTTACTGA AACAAAAAGG CCAAGATCAA 
GATGGTACTT CCATTCCTCC ATTTGAACCT CCAGGACCTG GGAGCTATGA AAATCTATCC 
AGTGGCACAG GAGAATCTAA ACCAGATGCT CTTGGAGGGA AACAGGAAGC CAGTGCAGAC 
TGTCAGGATG TTATTTCTAT GACAGCTAAC CGGCTAAGTG CTGTCATTCA AGCTTTTTAC 
ATGTGCTGTT CTTGTCAGAT GCCTCCAGGA ATGACCTCAC CCCGTTTCAT TGAATTTGTC 
TGCAAACATG ATGAAGTTTT AAAATGCTTT GTTAATAGAA ATCCCAAAAT TATATTTGAT 
CACTTTCATT TCCTCCTTGA ATGTCCTGAG TTGATGTCAA GATTCATGCA TATCATAAAA 
GCACAGCCAT TTAAAGATCG CTGTGAGTGG TTCTATGAAC ATTTGCACTC AGGACAACCA 
GATTCTGACA TGGTGCACAG GCCAGTGAAT GAAAATGATA TCCTGCTTGT TCACAGAGAT 
TCTATTTTTA GGAGTAGCTG TGAAGTTGTG TCAAAAGCAA ACTGTGCAAA GCTAAAGCAA 
GGAATTGCTG TACGGTTCCA TGGAGAAGAA GGCATGGGCC AAGGTGTTGT GCGTGAGTGG 
TTTGATATTC TGTCTAATGA GATAGTGAAT CCTGATTATG CATTGTTCAC CCAGTCAGCT 
GATGGAACAA CTTTTCAGCC TAATAGTAAT TCCTATGTAA ATCCTGATCA CTTGAACTAT 
TTCCGGTTTG CTGGACAGAT CTTGGGATTA GCTTTGAACC ACAGGCAGCT GGTCAACATT 
TACTTCACAA GATCGTTCTA TAAGCACATT CTTGGTATCC CTGTAAATTA CCAAGATGTG 
GCATCCATTG ATCCAGAATA TGCCAAAAAT TTGCAGTGGA TTTTAGATAA TGACATTAGT 
GATCTGGGTC TAGAACTAAC CTTTTCTGTT GAAACTGATG TGTTTGGAGC AATGGAAGAG 
GTGCCTTTGA AACCTGGGGG TGGAAGTATT CTTGTGACAC AAAATAACAA AGCGGAGTAT 
GTCCAGCTTG TTACTGAACT TCGAATGACA AGAGCCATTC AACCTCAGAT CAATGCTTTT 
TTACAGGGCT TTCATATGTT CATTCCACCT TCCCTCATAC AACTTTTTGA TGAATATGAA 
TTGGAGCTAC TGCTTTCTGG CATGCCAGAA ATTGATGTGA GCGATTGGAT AAGAAATACA 
GAATACACAA GTGGCTATGA AAGAGAAGAT CCAGTTATTC AGTGGTTCTG GGAAGTTGTG 
GAAGGCATTA CTCCAGAGGA GAGAGTTCTT CTCTTGCAGT TTGTTACTGG CAGTTCCAGG 
GTCCCACATG GTGGATTTGC TAACATCATG GGTGGAAGTG GATTGCAAAA CTTTACAATT 
GCTGCTGTGC CATATACTCC AAATCTCCTA CCAACTTCAA GCACATGCAT CAACATGCTC 
AAGTTACCTG AATACCCAAG TAAAGAAATA CTCAAGGACA GACTTCTTGT GGCGTTACAT 
TGTGGCAGCT ATGGTTACAC AATGGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( XM_849139.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_854232.1 Canis lupus familiaris HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe15637
Clone ID Related Accession (Same CDS sequence)	XM_849139.4 , XM_849139.5
Accession Version	XM_849139.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase HACE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_849139.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAG CGATGGAGCA GCTCAACCGC CTCACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGCTGC CGGACGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT 
GATCAACACA GGTCTGTTTC TGAGCTACTA TCAAATTCAA AGTTTGATGT CAATTATGCT 
TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC 
TTGGTCCTGC TATTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC AGGATGTACA 
CCCCTTCATT TGGCAGCTAG AAATGGGCAG AAGAAATGCA TGAGTAAATT ATTAGAATAC 
AGTGCTGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAATACACTG GCTCGCTGTG 
AATGGGCGGA CAGAACTACT CCATGACCTT GTGCAGCACG TCAGTGACGT CGACGTTGAG 
GATGCGATGG GGCAGACAGC ACTGCATGTC GCCTGCCAGA ATGGTCACAA GACGACAGTG 
CAGTGCTTGC TAGACAGTGG TGCTGATATT AACAGGCCAA ATGTATCTGG AGCCACTCCA 
TTGTACTTTG CTTGCAGTCA TGGTCAGAGA GACACAGCAC AAATTCTTCT GTTACGAGGA 
GCTAAATATC TGTCAGATAA AAATGGAGTA ACGCCTCTGG ATTTATGTGT ACAGGGTGGC 
TATGGAGAGA CTTGTGAAGT ATTAATTCAG TATCACCCTA GGCTTTTCCA GACAATTATT 
CAAATGACAC AGAATGAAGA TCTCCGAGAA AACATGTTAC GGCAAGTTCT GGAACATTTG 
TCTCAGCAAA GTGAAAGCCA GTACCTAAAG ATTCTAACAA GCCTTGCTGA AGTTGCCACA 
ACAAATGGGC ATAAACTGCT TAGTCTGTCT AGTAATTATG ATGCTCAGAT GAAGAGCCTC 
TTAAGGATTG TGAGGATATT TTGTCATGTC TTTCGAATTG GTCCATCCTC TCCCAGTAAT 
GGAATTGATT TGGGCTACAA CGGGAATAAA ACTCCAAGAA GCCAAGTGTT CAAGCCTCTT 
GAATTGCTTT GGCACTCATT AGATGAATGG CTAGTTTTAA TAGCTACAGA ATTGATGAAA 
AACAAAAAGG ACTCAACGGA TATCACTTCT ATTTTACTGA AACAAAAAGG CCAAGATCAA 
GATGGTACTT CCATTCCTCC ATTTGAACCT CCAGGACCTG GGAGCTATGA AAATCTATCC 
AGTGGCACAG GAGAATCTAA ACCAGATGCT CTTGGAGGGA AACAGGAAGC CAGTGCAGAC 
TGTCAGGATG TTATTTCTAT GACAGCTAAC CGGCTAAGTG CTGTCATTCA AGCTTTTTAC 
ATGTGCTGTT CTTGTCAGAT GCCTCCAGGA ATGACCTCAC CCCGTTTCAT TGAATTTGTC 
TGCAAACATG ATGAAGTTTT AAAATGCTTT GTTAATAGAA ATCCCAAAAT TATATTTGAT 
CACTTTCATT TCCTCCTTGA ATGTCCTGAG TTGATGTCAA GATTCATGCA TATCATAAAA 
GCACAGCCAT TTAAAGATCG CTGTGAGTGG TTCTATGAAC ATTTGCACTC AGGACAACCA 
GATTCTGACA TGGTGCACAG GCCAGTGAAT GAAAATGATA TCCTGCTTGT TCACAGAGAT 
TCTATTTTTA GGAGTAGCTG TGAAGTTGTG TCAAAAGCAA ACTGTGCAAA GCTAAAGCAA 
GGAATTGCTG TACGGTTCCA TGGAGAAGAA GGCATGGGCC AAGGTGTTGT GCGTGAGTGG 
TTTGATATTC TGTCTAATGA GATAGTGAAT CCTGATTATG CATTGTTCAC CCAGTCAGCT 
GATGGAACAA CTTTTCAGCC TAATAGTAAT TCCTATGTAA ATCCTGATCA CTTGAACTAT 
TTCCGGTTTG CTGGACAGAT CTTGGGATTA GCTTTGAACC ACAGGCAGCT GGTCAACATT 
TACTTCACAA GATCGTTCTA TAAGCACATT CTTGGTATCC CTGTAAATTA CCAAGATGTG 
GCATCCATTG ATCCAGAATA TGCCAAAAAT TTGCAGTGGA TTTTAGATAA TGACATTAGT 
GATCTGGGTC TAGAACTAAC CTTTTCTGTT GAAACTGATG TGTTTGGAGC AATGGAAGAG 
GTGCCTTTGA AACCTGGGGG TGGAAGTATT CTTGTGACAC AAAATAACAA AGCGGAGTAT 
GTCCAGCTTG TTACTGAACT TCGAATGACA AGAGCCATTC AACCTCAGAT CAATGCTTTT 
TTACAGGGCT TTCATATGTT CATTCCACCT TCCCTCATAC AACTTTTTGA TGAATATGAA 
TTGGAGCTAC TGCTTTCTGG CATGCCAGAA ATTGATGTGA GCGATTGGAT AAGAAATACA 
GAATACACAA GTGGCTATGA AAGAGAAGAT CCAGTTATTC AGTGGTTCTG GGAAGTTGTG 
GAAGGCATTA CTCCAGAGGA GAGAGTTCTT CTCTTGCAGT TTGTTACTGG CAGTTCCAGG 
GTCCCACATG GTGGATTTGC TAACATCATG GGTGGAAGTG GATTGCAAAA CTTTACAATT 
GCTGCTGTGC CATATACTCC AAATCTCCTA CCAACTTCAA GCACATGCAT CAACATGCTC 
AAGTTACCTG AATACCCAAG TAAAGAAATA CTCAAGGACA GACTTCTTGT GGCGTTACAT 
TGTGGCAGCT ATGGTTACAC AATGGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_854232.1
CDS	80..2809
Translation	MERAMEQLNRLTRSLRRARTVELPDDNETAVYTLMPMVMADQHRSVSELLSNSKFDVNYAFGRVKRSLLHIAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTAIHWLAVNGRTELLHDLVQHVSDVDVEDAMGQTALHVACQNGHKTTVQCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLLRGAKYLSDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRIFCHVFRIGPSSPSNGIDLGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKKDSTDITSILLKQKGQDQDGTSIPPFEPPGPGSYENLSSGTGESKPDALGGKQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIRNTEYTSGYEREDPVIQWFWEVVEGITPEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	XM_849139.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_849139.5

ORF Insert Sequence:

ATGGAGAGAG CGATGGAGCA GCTCAACCGC CTCACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGCTGC CGGACGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT 
GATCAACACA GGTCTGTTTC TGAGCTACTA TCAAATTCAA AGTTTGATGT CAATTATGCT 
TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC 
TTGGTCCTGC TATTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC AGGATGTACA 
CCCCTTCATT TGGCAGCTAG AAATGGGCAG AAGAAATGCA TGAGTAAATT ATTAGAATAC 
AGTGCTGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAATACACTG GCTCGCTGTG 
AATGGGCGGA CAGAACTACT CCATGACCTT GTGCAGCACG TCAGTGACGT CGACGTTGAG 
GATGCGATGG GGCAGACAGC ACTGCATGTC GCCTGCCAGA ATGGTCACAA GACGACAGTG 
CAGTGCTTGC TAGACAGTGG TGCTGATATT AACAGGCCAA ATGTATCTGG AGCCACTCCA 
TTGTACTTTG CTTGCAGTCA TGGTCAGAGA GACACAGCAC AAATTCTTCT GTTACGAGGA 
GCTAAATATC TGTCAGATAA AAATGGAGTA ACGCCTCTGG ATTTATGTGT ACAGGGTGGC 
TATGGAGAGA CTTGTGAAGT ATTAATTCAG TATCACCCTA GGCTTTTCCA GACAATTATT 
CAAATGACAC AGAATGAAGA TCTCCGAGAA AACATGTTAC GGCAAGTTCT GGAACATTTG 
TCTCAGCAAA GTGAAAGCCA GTACCTAAAG ATTCTAACAA GCCTTGCTGA AGTTGCCACA 
ACAAATGGGC ATAAACTGCT TAGTCTGTCT AGTAATTATG ATGCTCAGAT GAAGAGCCTC 
TTAAGGATTG TGAGGATATT TTGTCATGTC TTTCGAATTG GTCCATCCTC TCCCAGTAAT 
GGAATTGATT TGGGCTACAA CGGGAATAAA ACTCCAAGAA GCCAAGTGTT CAAGCCTCTT 
GAATTGCTTT GGCACTCATT AGATGAATGG CTAGTTTTAA TAGCTACAGA ATTGATGAAA 
AACAAAAAGG ACTCAACGGA TATCACTTCT ATTTTACTGA AACAAAAAGG CCAAGATCAA 
GATGGTACTT CCATTCCTCC ATTTGAACCT CCAGGACCTG GGAGCTATGA AAATCTATCC 
AGTGGCACAG GAGAATCTAA ACCAGATGCT CTTGGAGGGA AACAGGAAGC CAGTGCAGAC 
TGTCAGGATG TTATTTCTAT GACAGCTAAC CGGCTAAGTG CTGTCATTCA AGCTTTTTAC 
ATGTGCTGTT CTTGTCAGAT GCCTCCAGGA ATGACCTCAC CCCGTTTCAT TGAATTTGTC 
TGCAAACATG ATGAAGTTTT AAAATGCTTT GTTAATAGAA ATCCCAAAAT TATATTTGAT 
CACTTTCATT TCCTCCTTGA ATGTCCTGAG TTGATGTCAA GATTCATGCA TATCATAAAA 
GCACAGCCAT TTAAAGATCG CTGTGAGTGG TTCTATGAAC ATTTGCACTC AGGACAACCA 
GATTCTGACA TGGTGCACAG GCCAGTGAAT GAAAATGATA TCCTGCTTGT TCACAGAGAT 
TCTATTTTTA GGAGTAGCTG TGAAGTTGTG TCAAAAGCAA ACTGTGCAAA GCTAAAGCAA 
GGAATTGCTG TACGGTTCCA TGGAGAAGAA GGCATGGGCC AAGGTGTTGT GCGTGAGTGG 
TTTGATATTC TGTCTAATGA GATAGTGAAT CCTGATTATG CATTGTTCAC CCAGTCAGCT 
GATGGAACAA CTTTTCAGCC TAATAGTAAT TCCTATGTAA ATCCTGATCA CTTGAACTAT 
TTCCGGTTTG CTGGACAGAT CTTGGGATTA GCTTTGAACC ACAGGCAGCT GGTCAACATT 
TACTTCACAA GATCGTTCTA TAAGCACATT CTTGGTATCC CTGTAAATTA CCAAGATGTG 
GCATCCATTG ATCCAGAATA TGCCAAAAAT TTGCAGTGGA TTTTAGATAA TGACATTAGT 
GATCTGGGTC TAGAACTAAC CTTTTCTGTT GAAACTGATG TGTTTGGAGC AATGGAAGAG 
GTGCCTTTGA AACCTGGGGG TGGAAGTATT CTTGTGACAC AAAATAACAA AGCGGAGTAT 
GTCCAGCTTG TTACTGAACT TCGAATGACA AGAGCCATTC AACCTCAGAT CAATGCTTTT 
TTACAGGGCT TTCATATGTT CATTCCACCT TCCCTCATAC AACTTTTTGA TGAATATGAA 
TTGGAGCTAC TGCTTTCTGG CATGCCAGAA ATTGATGTGA GCGATTGGAT AAGAAATACA 
GAATACACAA GTGGCTATGA AAGAGAAGAT CCAGTTATTC AGTGGTTCTG GGAAGTTGTG 
GAAGGCATTA CTCCAGAGGA GAGAGTTCTT CTCTTGCAGT TTGTTACTGG CAGTTCCAGG 
GTCCCACATG GTGGATTTGC TAACATCATG GGTGGAAGTG GATTGCAAAA CTTTACAATT 
GCTGCTGTGC CATATACTCC AAATCTCCTA CCAACTTCAA GCACATGCAT CAACATGCTC 
AAGTTACCTG AATACCCAAG TAAAGAAATA CTCAAGGACA GACTTCTTGT GGCGTTACAT 
TGTGGCAGCT ATGGTTACAC AATGGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( XM_005627667.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005627724.1 Canis lupus familiaris HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe15638
Clone ID Related Accession (Same CDS sequence)	XM_005627667.2 , XM_005627667.3
Accession Version	XM_005627667.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase HACE1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005627667.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAG CGATGGAGCA GCTCAACCGC CTCACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGCTGC CGGACGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT 
GATCAACACA GGTCTGTTTC TGAGCTACTA TCAAATTCAA AGTTTGATGT CAATTATGCT 
TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC 
TTGGTCCTGC TATTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC AGGATGTACA 
CCCCTTCATT TGGCAGCTAG AAATGGGCAG AAGAAATGCA TGAGTAAATT ATTAGAATAC 
AGTGCTGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAACAGTGCA GTGCTTGCTA 
GACAGTGGTG CTGATATTAA CAGGCCAAAT GTATCTGGAG CCACTCCATT GTACTTTGCT 
TGCAGTCATG GTCAGAGAGA CACAGCACAA ATTCTTCTGT TACGAGGAGC TAAATATCTG 
TCAGATAAAA ATGGAGTAAC GCCTCTGGAT TTATGTGTAC AGGGTGGCTA TGGAGAGACT 
TGTGAAGTAT TAATTCAGTA TCACCCTAGG CTTTTCCAGA CAATTATTCA AATGACACAG 
AATGAAGATC TCCGAGAAAA CATGTTACGG CAAGTTCTGG AACATTTGTC TCAGCAAAGT 
GAAAGCCAGT ACCTAAAGAT TCTAACAAGC CTTGCTGAAG TTGCCACAAC AAATGGGCAT 
AAACTGCTTA GTCTGTCTAG TAATTATGAT GCTCAGATGA AGAGCCTCTT AAGGATTGTG 
AGGATATTTT GTCATGTCTT TCGAATTGGT CCATCCTCTC CCAGTAATGG AATTGATTTG 
GGCTACAACG GGAATAAAAC TCCAAGAAGC CAAGTGTTCA AGCCTCTTGA ATTGCTTTGG 
CACTCATTAG ATGAATGGCT AGTTTTAATA GCTACAGAAT TGATGAAAAA CAAAAAGGAC 
TCAACGGATA TCACTTCTAT TTTACTGAAA CAAAAAGGCC AAGATCAAGA TGGTACTTCC 
ATTCCTCCAT TTGAACCTCC AGGACCTGGG AGCTATGAAA ATCTATCCAG TGGCACAGGA 
GAATCTAAAC CAGATGCTCT TGGAGGGAAA CAGGAAGCCA GTGCAGACTG TCAGGATGTT 
ATTTCTATGA CAGCTAACCG GCTAAGTGCT GTCATTCAAG CTTTTTACAT GTGCTGTTCT 
TGTCAGATGC CTCCAGGAAT GACCTCACCC CGTTTCATTG AATTTGTCTG CAAACATGAT 
GAAGTTTTAA AATGCTTTGT TAATAGAAAT CCCAAAATTA TATTTGATCA CTTTCATTTC 
CTCCTTGAAT GTCCTGAGTT GATGTCAAGA TTCATGCATA TCATAAAAGC ACAGCCATTT 
AAAGATCGCT GTGAGTGGTT CTATGAACAT TTGCACTCAG GACAACCAGA TTCTGACATG 
GTGCACAGGC CAGTGAATGA AAATGATATC CTGCTTGTTC ACAGAGATTC TATTTTTAGG 
AGTAGCTGTG AAGTTGTGTC AAAAGCAAAC TGTGCAAAGC TAAAGCAAGG AATTGCTGTA 
CGGTTCCATG GAGAAGAAGG CATGGGCCAA GGTGTTGTGC GTGAGTGGTT TGATATTCTG 
TCTAATGAGA TAGTGAATCC TGATTATGCA TTGTTCACCC AGTCAGCTGA TGGAACAACT 
TTTCAGCCTA ATAGTAATTC CTATGTAAAT CCTGATCACT TGAACTATTT CCGGTTTGCT 
GGACAGATCT TGGGATTAGC TTTGAACCAC AGGCAGCTGG TCAACATTTA CTTCACAAGA 
TCGTTCTATA AGCACATTCT TGGTATCCCT GTAAATTACC AAGATGTGGC ATCCATTGAT 
CCAGAATATG CCAAAAATTT GCAGTGGATT TTAGATAATG ACATTAGTGA TCTGGGTCTA 
GAACTAACCT TTTCTGTTGA AACTGATGTG TTTGGAGCAA TGGAAGAGGT GCCTTTGAAA 
CCTGGGGGTG GAAGTATTCT TGTGACACAA AATAACAAAG CGGAGTATGT CCAGCTTGTT 
ACTGAACTTC GAATGACAAG AGCCATTCAA CCTCAGATCA ATGCTTTTTT ACAGGGCTTT 
CATATGTTCA TTCCACCTTC CCTCATACAA CTTTTTGATG AATATGAATT GGAGCTACTG 
CTTTCTGGCA TGCCAGAAAT TGATGTGAGC GATTGGATAA GAAATACAGA ATACACAAGT 
GGCTATGAAA GAGAAGATCC AGTTATTCAG TGGTTCTGGG AAGTTGTGGA AGGCATTACT 
CCAGAGGAGA GAGTTCTTCT CTTGCAGTTT GTTACTGGCA GTTCCAGGGT CCCACATGGT 
GGATTTGCTA ACATCATGGG TGGAAGTGGA TTGCAAAACT TTACAATTGC TGCTGTGCCA 
TATACTCCAA ATCTCCTACC AACTTCAAGC ACATGCATCA ACATGCTCAA GTTACCTGAA 
TACCCAAGTA AAGAAATACT CAAGGACAGA CTTCTTGTGG CGTTACATTG TGGCAGCTAT 
GGTTACACAA TGGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005627724.1
CDS	190..2787
Translation	MERAMEQLNRLTRSLRRARTVELPDDNETAVYTLMPMVMADQHRSVSELLSNSKFDVNYAFGRVKRSLLHIAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTATVQCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLLRGAKYLSDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRIFCHVFRIGPSSPSNGIDLGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKKDSTDITSILLKQKGQDQDGTSIPPFEPPGPGSYENLSSGTGESKPDALGGKQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIRNTEYTSGYEREDPVIQWFWEVVEGITPEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	XM_005627667.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005627667.2

ORF Insert Sequence:

ATGGAGAGAG CGATGGAGCA GCTCAACCGC CTCACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGCTGC CGGACGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT 
GATCAACACA GGTCTGTTTC TGAGCTACTA TCAAATTCAA AGTTTGATGT CAATTATGCT 
TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC 
TTGGTCCTGC TATTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC AGGATGTACA 
CCCCTTCATT TGGCAGCTAG AAATGGGCAG AAGAAATGCA TGAGTAAATT ATTAGAATAC 
AGTGCTGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAACAGTGCA GTGCTTGCTA 
GACAGTGGTG CTGATATTAA CAGGCCAAAT GTATCTGGAG CCACTCCATT GTACTTTGCT 
TGCAGTCATG GTCAGAGAGA CACAGCACAA ATTCTTCTGT TACGAGGAGC TAAATATCTG 
TCAGATAAAA ATGGAGTAAC GCCTCTGGAT TTATGTGTAC AGGGTGGCTA TGGAGAGACT 
TGTGAAGTAT TAATTCAGTA TCACCCTAGG CTTTTCCAGA CAATTATTCA AATGACACAG 
AATGAAGATC TCCGAGAAAA CATGTTACGG CAAGTTCTGG AACATTTGTC TCAGCAAAGT 
GAAAGCCAGT ACCTAAAGAT TCTAACAAGC CTTGCTGAAG TTGCCACAAC AAATGGGCAT 
AAACTGCTTA GTCTGTCTAG TAATTATGAT GCTCAGATGA AGAGCCTCTT AAGGATTGTG 
AGGATATTTT GTCATGTCTT TCGAATTGGT CCATCCTCTC CCAGTAATGG AATTGATTTG 
GGCTACAACG GGAATAAAAC TCCAAGAAGC CAAGTGTTCA AGCCTCTTGA ATTGCTTTGG 
CACTCATTAG ATGAATGGCT AGTTTTAATA GCTACAGAAT TGATGAAAAA CAAAAAGGAC 
TCAACGGATA TCACTTCTAT TTTACTGAAA CAAAAAGGCC AAGATCAAGA TGGTACTTCC 
ATTCCTCCAT TTGAACCTCC AGGACCTGGG AGCTATGAAA ATCTATCCAG TGGCACAGGA 
GAATCTAAAC CAGATGCTCT TGGAGGGAAA CAGGAAGCCA GTGCAGACTG TCAGGATGTT 
ATTTCTATGA CAGCTAACCG GCTAAGTGCT GTCATTCAAG CTTTTTACAT GTGCTGTTCT 
TGTCAGATGC CTCCAGGAAT GACCTCACCC CGTTTCATTG AATTTGTCTG CAAACATGAT 
GAAGTTTTAA AATGCTTTGT TAATAGAAAT CCCAAAATTA TATTTGATCA CTTTCATTTC 
CTCCTTGAAT GTCCTGAGTT GATGTCAAGA TTCATGCATA TCATAAAAGC ACAGCCATTT 
AAAGATCGCT GTGAGTGGTT CTATGAACAT TTGCACTCAG GACAACCAGA TTCTGACATG 
GTGCACAGGC CAGTGAATGA AAATGATATC CTGCTTGTTC ACAGAGATTC TATTTTTAGG 
AGTAGCTGTG AAGTTGTGTC AAAAGCAAAC TGTGCAAAGC TAAAGCAAGG AATTGCTGTA 
CGGTTCCATG GAGAAGAAGG CATGGGCCAA GGTGTTGTGC GTGAGTGGTT TGATATTCTG 
TCTAATGAGA TAGTGAATCC TGATTATGCA TTGTTCACCC AGTCAGCTGA TGGAACAACT 
TTTCAGCCTA ATAGTAATTC CTATGTAAAT CCTGATCACT TGAACTATTT CCGGTTTGCT 
GGACAGATCT TGGGATTAGC TTTGAACCAC AGGCAGCTGG TCAACATTTA CTTCACAAGA 
TCGTTCTATA AGCACATTCT TGGTATCCCT GTAAATTACC AAGATGTGGC ATCCATTGAT 
CCAGAATATG CCAAAAATTT GCAGTGGATT TTAGATAATG ACATTAGTGA TCTGGGTCTA 
GAACTAACCT TTTCTGTTGA AACTGATGTG TTTGGAGCAA TGGAAGAGGT GCCTTTGAAA 
CCTGGGGGTG GAAGTATTCT TGTGACACAA AATAACAAAG CGGAGTATGT CCAGCTTGTT 
ACTGAACTTC GAATGACAAG AGCCATTCAA CCTCAGATCA ATGCTTTTTT ACAGGGCTTT 
CATATGTTCA TTCCACCTTC CCTCATACAA CTTTTTGATG AATATGAATT GGAGCTACTG 
CTTTCTGGCA TGCCAGAAAT TGATGTGAGC GATTGGATAA GAAATACAGA ATACACAAGT 
GGCTATGAAA GAGAAGATCC AGTTATTCAG TGGTTCTGGG AAGTTGTGGA AGGCATTACT 
CCAGAGGAGA GAGTTCTTCT CTTGCAGTTT GTTACTGGCA GTTCCAGGGT CCCACATGGT 
GGATTTGCTA ACATCATGGG TGGAAGTGGA TTGCAAAACT TTACAATTGC TGCTGTGCCA 
TATACTCCAA ATCTCCTACC AACTTCAAGC ACATGCATCA ACATGCTCAA GTTACCTGAA 
TACCCAAGTA AAGAAATACT CAAGGACAGA CTTCTTGTGG CGTTACATTG TGGCAGCTAT 
GGTTACACAA TGGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( XM_005627667.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005627724.1 Canis lupus familiaris HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe15638
Clone ID Related Accession (Same CDS sequence)	XM_005627667.2 , XM_005627667.3
Accession Version	XM_005627667.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase HACE1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005627667.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAG CGATGGAGCA GCTCAACCGC CTCACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGCTGC CGGACGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT 
GATCAACACA GGTCTGTTTC TGAGCTACTA TCAAATTCAA AGTTTGATGT CAATTATGCT 
TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC 
TTGGTCCTGC TATTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC AGGATGTACA 
CCCCTTCATT TGGCAGCTAG AAATGGGCAG AAGAAATGCA TGAGTAAATT ATTAGAATAC 
AGTGCTGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAACAGTGCA GTGCTTGCTA 
GACAGTGGTG CTGATATTAA CAGGCCAAAT GTATCTGGAG CCACTCCATT GTACTTTGCT 
TGCAGTCATG GTCAGAGAGA CACAGCACAA ATTCTTCTGT TACGAGGAGC TAAATATCTG 
TCAGATAAAA ATGGAGTAAC GCCTCTGGAT TTATGTGTAC AGGGTGGCTA TGGAGAGACT 
TGTGAAGTAT TAATTCAGTA TCACCCTAGG CTTTTCCAGA CAATTATTCA AATGACACAG 
AATGAAGATC TCCGAGAAAA CATGTTACGG CAAGTTCTGG AACATTTGTC TCAGCAAAGT 
GAAAGCCAGT ACCTAAAGAT TCTAACAAGC CTTGCTGAAG TTGCCACAAC AAATGGGCAT 
AAACTGCTTA GTCTGTCTAG TAATTATGAT GCTCAGATGA AGAGCCTCTT AAGGATTGTG 
AGGATATTTT GTCATGTCTT TCGAATTGGT CCATCCTCTC CCAGTAATGG AATTGATTTG 
GGCTACAACG GGAATAAAAC TCCAAGAAGC CAAGTGTTCA AGCCTCTTGA ATTGCTTTGG 
CACTCATTAG ATGAATGGCT AGTTTTAATA GCTACAGAAT TGATGAAAAA CAAAAAGGAC 
TCAACGGATA TCACTTCTAT TTTACTGAAA CAAAAAGGCC AAGATCAAGA TGGTACTTCC 
ATTCCTCCAT TTGAACCTCC AGGACCTGGG AGCTATGAAA ATCTATCCAG TGGCACAGGA 
GAATCTAAAC CAGATGCTCT TGGAGGGAAA CAGGAAGCCA GTGCAGACTG TCAGGATGTT 
ATTTCTATGA CAGCTAACCG GCTAAGTGCT GTCATTCAAG CTTTTTACAT GTGCTGTTCT 
TGTCAGATGC CTCCAGGAAT GACCTCACCC CGTTTCATTG AATTTGTCTG CAAACATGAT 
GAAGTTTTAA AATGCTTTGT TAATAGAAAT CCCAAAATTA TATTTGATCA CTTTCATTTC 
CTCCTTGAAT GTCCTGAGTT GATGTCAAGA TTCATGCATA TCATAAAAGC ACAGCCATTT 
AAAGATCGCT GTGAGTGGTT CTATGAACAT TTGCACTCAG GACAACCAGA TTCTGACATG 
GTGCACAGGC CAGTGAATGA AAATGATATC CTGCTTGTTC ACAGAGATTC TATTTTTAGG 
AGTAGCTGTG AAGTTGTGTC AAAAGCAAAC TGTGCAAAGC TAAAGCAAGG AATTGCTGTA 
CGGTTCCATG GAGAAGAAGG CATGGGCCAA GGTGTTGTGC GTGAGTGGTT TGATATTCTG 
TCTAATGAGA TAGTGAATCC TGATTATGCA TTGTTCACCC AGTCAGCTGA TGGAACAACT 
TTTCAGCCTA ATAGTAATTC CTATGTAAAT CCTGATCACT TGAACTATTT CCGGTTTGCT 
GGACAGATCT TGGGATTAGC TTTGAACCAC AGGCAGCTGG TCAACATTTA CTTCACAAGA 
TCGTTCTATA AGCACATTCT TGGTATCCCT GTAAATTACC AAGATGTGGC ATCCATTGAT 
CCAGAATATG CCAAAAATTT GCAGTGGATT TTAGATAATG ACATTAGTGA TCTGGGTCTA 
GAACTAACCT TTTCTGTTGA AACTGATGTG TTTGGAGCAA TGGAAGAGGT GCCTTTGAAA 
CCTGGGGGTG GAAGTATTCT TGTGACACAA AATAACAAAG CGGAGTATGT CCAGCTTGTT 
ACTGAACTTC GAATGACAAG AGCCATTCAA CCTCAGATCA ATGCTTTTTT ACAGGGCTTT 
CATATGTTCA TTCCACCTTC CCTCATACAA CTTTTTGATG AATATGAATT GGAGCTACTG 
CTTTCTGGCA TGCCAGAAAT TGATGTGAGC GATTGGATAA GAAATACAGA ATACACAAGT 
GGCTATGAAA GAGAAGATCC AGTTATTCAG TGGTTCTGGG AAGTTGTGGA AGGCATTACT 
CCAGAGGAGA GAGTTCTTCT CTTGCAGTTT GTTACTGGCA GTTCCAGGGT CCCACATGGT 
GGATTTGCTA ACATCATGGG TGGAAGTGGA TTGCAAAACT TTACAATTGC TGCTGTGCCA 
TATACTCCAA ATCTCCTACC AACTTCAAGC ACATGCATCA ACATGCTCAA GTTACCTGAA 
TACCCAAGTA AAGAAATACT CAAGGACAGA CTTCTTGTGG CGTTACATTG TGGCAGCTAT 
GGTTACACAA TGGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005627724.1
CDS	80..2677
Translation	MERAMEQLNRLTRSLRRARTVELPDDNETAVYTLMPMVMADQHRSVSELLSNSKFDVNYAFGRVKRSLLHIAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTATVQCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLLRGAKYLSDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRIFCHVFRIGPSSPSNGIDLGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKKDSTDITSILLKQKGQDQDGTSIPPFEPPGPGSYENLSSGTGESKPDALGGKQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIRNTEYTSGYEREDPVIQWFWEVVEGITPEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	XM_005627667.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005627667.3

ORF Insert Sequence:

ATGGAGAGAG CGATGGAGCA GCTCAACCGC CTCACGCGCT CGCTGCGCCG CGCGCGCACC 
GTGGAGCTGC CGGACGATAA TGAAACTGCT GTTTATACAT TAATGCCAAT GGTTATGGCT 
GATCAACACA GGTCTGTTTC TGAGCTACTA TCAAATTCAA AGTTTGATGT CAATTATGCT 
TTTGGACGTG TGAAAAGAAG CTTGCTTCAC ATTGCAGCAA ATTGTGGATC AGTGGAATGC 
TTGGTCCTGC TATTAAAGAA AGGAGCAAAT CCTAACTATC AAGATATTTC AGGATGTACA 
CCCCTTCATT TGGCAGCTAG AAATGGGCAG AAGAAATGCA TGAGTAAATT ATTAGAATAC 
AGTGCTGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAACAGTGCA GTGCTTGCTA 
GACAGTGGTG CTGATATTAA CAGGCCAAAT GTATCTGGAG CCACTCCATT GTACTTTGCT 
TGCAGTCATG GTCAGAGAGA CACAGCACAA ATTCTTCTGT TACGAGGAGC TAAATATCTG 
TCAGATAAAA ATGGAGTAAC GCCTCTGGAT TTATGTGTAC AGGGTGGCTA TGGAGAGACT 
TGTGAAGTAT TAATTCAGTA TCACCCTAGG CTTTTCCAGA CAATTATTCA AATGACACAG 
AATGAAGATC TCCGAGAAAA CATGTTACGG CAAGTTCTGG AACATTTGTC TCAGCAAAGT 
GAAAGCCAGT ACCTAAAGAT TCTAACAAGC CTTGCTGAAG TTGCCACAAC AAATGGGCAT 
AAACTGCTTA GTCTGTCTAG TAATTATGAT GCTCAGATGA AGAGCCTCTT AAGGATTGTG 
AGGATATTTT GTCATGTCTT TCGAATTGGT CCATCCTCTC CCAGTAATGG AATTGATTTG 
GGCTACAACG GGAATAAAAC TCCAAGAAGC CAAGTGTTCA AGCCTCTTGA ATTGCTTTGG 
CACTCATTAG ATGAATGGCT AGTTTTAATA GCTACAGAAT TGATGAAAAA CAAAAAGGAC 
TCAACGGATA TCACTTCTAT TTTACTGAAA CAAAAAGGCC AAGATCAAGA TGGTACTTCC 
ATTCCTCCAT TTGAACCTCC AGGACCTGGG AGCTATGAAA ATCTATCCAG TGGCACAGGA 
GAATCTAAAC CAGATGCTCT TGGAGGGAAA CAGGAAGCCA GTGCAGACTG TCAGGATGTT 
ATTTCTATGA CAGCTAACCG GCTAAGTGCT GTCATTCAAG CTTTTTACAT GTGCTGTTCT 
TGTCAGATGC CTCCAGGAAT GACCTCACCC CGTTTCATTG AATTTGTCTG CAAACATGAT 
GAAGTTTTAA AATGCTTTGT TAATAGAAAT CCCAAAATTA TATTTGATCA CTTTCATTTC 
CTCCTTGAAT GTCCTGAGTT GATGTCAAGA TTCATGCATA TCATAAAAGC ACAGCCATTT 
AAAGATCGCT GTGAGTGGTT CTATGAACAT TTGCACTCAG GACAACCAGA TTCTGACATG 
GTGCACAGGC CAGTGAATGA AAATGATATC CTGCTTGTTC ACAGAGATTC TATTTTTAGG 
AGTAGCTGTG AAGTTGTGTC AAAAGCAAAC TGTGCAAAGC TAAAGCAAGG AATTGCTGTA 
CGGTTCCATG GAGAAGAAGG CATGGGCCAA GGTGTTGTGC GTGAGTGGTT TGATATTCTG 
TCTAATGAGA TAGTGAATCC TGATTATGCA TTGTTCACCC AGTCAGCTGA TGGAACAACT 
TTTCAGCCTA ATAGTAATTC CTATGTAAAT CCTGATCACT TGAACTATTT CCGGTTTGCT 
GGACAGATCT TGGGATTAGC TTTGAACCAC AGGCAGCTGG TCAACATTTA CTTCACAAGA 
TCGTTCTATA AGCACATTCT TGGTATCCCT GTAAATTACC AAGATGTGGC ATCCATTGAT 
CCAGAATATG CCAAAAATTT GCAGTGGATT TTAGATAATG ACATTAGTGA TCTGGGTCTA 
GAACTAACCT TTTCTGTTGA AACTGATGTG TTTGGAGCAA TGGAAGAGGT GCCTTTGAAA 
CCTGGGGGTG GAAGTATTCT TGTGACACAA AATAACAAAG CGGAGTATGT CCAGCTTGTT 
ACTGAACTTC GAATGACAAG AGCCATTCAA CCTCAGATCA ATGCTTTTTT ACAGGGCTTT 
CATATGTTCA TTCCACCTTC CCTCATACAA CTTTTTGATG AATATGAATT GGAGCTACTG 
CTTTCTGGCA TGCCAGAAAT TGATGTGAGC GATTGGATAA GAAATACAGA ATACACAAGT 
GGCTATGAAA GAGAAGATCC AGTTATTCAG TGGTTCTGGG AAGTTGTGGA AGGCATTACT 
CCAGAGGAGA GAGTTCTTCT CTTGCAGTTT GTTACTGGCA GTTCCAGGGT CCCACATGGT 
GGATTTGCTA ACATCATGGG TGGAAGTGGA TTGCAAAACT TTACAATTGC TGCTGTGCCA 
TATACTCCAA ATCTCCTACC AACTTCAAGC ACATGCATCA ACATGCTCAA GTTACCTGAA 
TACCCAAGTA AAGAAATACT CAAGGACAGA CTTCTTGTGG CGTTACATTG TGGCAGCTAT 
GGTTACACAA TGGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( XM_022426399.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022282107.1 Canis lupus familiaris HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe292658
Clone ID Related Accession (Same CDS sequence)	XM_022426399.1
Accession Version	XM_022426399.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase HACE1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAATGG TTATGGCTGA TCAACACAGG TCTGTTTCTG AGCTACTATC AAATTCAAAG 
TTTGATGTCA ATTATGCTTT TGGACGTGTG AAAAGAAGCT TGCTTCACAT TGCAGCAAAT 
TGTGGATCAG TGGAATGCTT GGTCCTGCTA TTAAAGAAAG GAGCAAATCC TAACTATCAA 
GATATTTCAG GATGTACACC CCTTCATTTG GCAGCTAGAA ATGGGCAGAA GAAATGCATG 
AGTAAATTAT TAGAATACAG TGCTGATGTC AACATTTGTA ATAATGAAGG CCTTACAGCA 
ATACACTGGC TCGCTGTGAA TGGGCGGACA GAACTACTCC ATGACCTTGT GCAGCACGTC 
AGTGACGTCG ACGTTGAGGA TGCGATGGGG CAGACAGCAC TGCATGTCGC CTGCCAGAAT 
GGTCACAAGA CGACAGTGCA GTGCTTGCTA GACAGTGGTG CTGATATTAA CAGGCCAAAT 
GTATCTGGAG CCACTCCATT GTACTTTGCT TGCAGTCATG GTCAGAGAGA CACAGCACAA 
ATTCTTCTGT TACGAGGAGC TAAATATCTG TCAGATAAAA ATGGAGTAAC GCCTCTGGAT 
TTATGTGTAC AGGGTGGCTA TGGAGAGACT TGTGAAGTAT TAATTCAGTA TCACCCTAGG 
CTTTTCCAGA CAATTATTCA AATGACACAG AATGAAGATC TCCGAGAAAA CATGTTACGG 
CAAGTTCTGG AACATTTGTC TCAGCAAAGT GAAAGCCAGT ACCTAAAGAT TCTAACAAGC 
CTTGCTGAAG TTGCCACAAC AAATGGGCAT AAACTGCTTA GTCTGTCTAG TAATTATGAT 
GCTCAGATGA AGAGCCTCTT AAGGATTGTG AGGATATTTT GTCATGTCTT TCGAATTGGT 
CCATCCTCTC CCAGTAATGG AATTGATTTG GGCTACAACG GGAATAAAAC TCCAAGAAGC 
CAAGTGTTCA AGCCTCTTGA ATTGCTTTGG CACTCATTAG ATGAATGGCT AGTTTTAATA 
GCTACAGAAT TGATGAAAAA CAAAAAGGAC TCAACGGATA TCACTTCTAT TTTACTGAAA 
CAAAAAGGCC AAGATCAAGA TGGTACTTCC ATTCCTCCAT TTGAACCTCC AGGACCTGGG 
AGCTATGAAA ATCTATCCAG TGGCACAGGA GAATCTAAAC CAGATGCTCT TGGAGGGAAA 
CAGGAAGCCA GTGCAGACTG TCAGGATGTT ATTTCTATGA CAGCTAACCG GCTAAGTGCT 
GTCATTCAAG CTTTTTACAT GTGCTGTTCT TGTCAGATGC CTCCAGGAAT GACCTCACCC 
CGTTTCATTG AATTTGTCTG CAAACATGAT GAAGTTTTAA AATGCTTTGT TAATAGAAAT 
CCCAAAATTA TATTTGATCA CTTTCATTTC CTCCTTGAAT GTCCTGAGTT GATGTCAAGA 
TTCATGCATA TCATAAAAGC ACAGCCATTT AAAGATCGCT GTGAGTGGTT CTATGAACAT 
TTGCACTCAG GACAACCAGA TTCTGACATG GTGCACAGGC CAGTGAATGA AAATGATATC 
CTGCTTGTTC ACAGAGATTC TATTTTTAGG AGTAGCTGTG AAGTTGTGTC AAAAGCAAAC 
TGTGCAAAGC TAAAGCAAGG AATTGCTGTA CGGTTCCATG GAGAAGAAGG CATGGGCCAA 
GGTGTTGTGC GTGAGTGGTT TGATATTCTG TCTAATGAGA TAGTGAATCC TGATTATGCA 
TTGTTCACCC AGTCAGCTGA TGGAACAACT TTTCAGCCTA ATAGTAATTC CTATGTAAAT 
CCTGATCACT TGAACTATTT CCGGTTTGCT GGACAGATCT TGGGATTAGC TTTGAACCAC 
AGGCAGCTGG TCAACATTTA CTTCACAAGA TCGTTCTATA AGCACATTCT TGGTATCCCT 
GTAAATTACC AAGATGTGGC ATCCATTGAT CCAGAATATG CCAAAAATTT GCAGTGGATT 
TTAGATAATG ACATTAGTGA TCTGGGTCTA GAACTAACCT TTTCTGTTGA AACTGATGTG 
TTTGGAGCAA TGGAAGAGGT GCCTTTGAAA CCTGGGGGTG GAAGTATTCT TGTGACACAA 
AATAACAAAG CGGAGTATGT CCAGCTTGTT ACTGAACTTC GAATGACAAG AGCCATTCAA 
CCTCAGATCA ATGCTTTTTT ACAGGGCTTT CATATGTTCA TTCCACCTTC CCTCATACAA 
CTTTTTGATG AATATGAATT GGAGCTACTG CTTTCTGGCA TGCCAGAAAT TGATGTGAGC 
GATTGGATAA GAAATACAGA ATACACAAGT GGCTATGAAA GAGAAGATCC AGTTATTCAG 
TGGTTCTGGG AAGTTGTGGA AGGCATTACT CCAGAGGAGA GAGTTCTTCT CTTGCAGTTT 
GTTACTGGCA GTTCCAGGGT CCCACATGGT GGATTTGCTA ACATCATGGG TGGAAGTGGA 
TTGCAAAACT TTACAATTGC TGCTGTGCCA TATACTCCAA ATCTCCTACC AACTTCAAGC 
ACATGCATCA ACATGCTCAA GTTACCTGAA TACCCAAGTA AAGAAATACT CAAGGACAGA 
CTTCTTGTGG CGTTACATTG TGGCAGCTAT GGTTACACAA TGGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022282107.1
CDS	46..2673
Translation	MPMVMADQHRSVSELLSNSKFDVNYAFGRVKRSLLHIAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTAIHWLAVNGRTELLHDLVQHVSDVDVEDAMGQTALHVACQNGHKTTVQCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLLRGAKYLSDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRIFCHVFRIGPSSPSNGIDLGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKKDSTDITSILLKQKGQDQDGTSIPPFEPPGPGSYENLSSGTGESKPDALGGKQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIRNTEYTSGYEREDPVIQWFWEVVEGITPEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	XM_022426399.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022426399.1

ORF Insert Sequence:

ATGCCAATGG TTATGGCTGA TCAACACAGG TCTGTTTCTG AGCTACTATC AAATTCAAAG 
TTTGATGTCA ATTATGCTTT TGGACGTGTG AAAAGAAGCT TGCTTCACAT TGCAGCAAAT 
TGTGGATCAG TGGAATGCTT GGTCCTGCTA TTAAAGAAAG GAGCAAATCC TAACTATCAA 
GATATTTCAG GATGTACACC CCTTCATTTG GCAGCTAGAA ATGGGCAGAA GAAATGCATG 
AGTAAATTAT TAGAATACAG TGCTGATGTC AACATTTGTA ATAATGAAGG CCTTACAGCA 
ATACACTGGC TCGCTGTGAA TGGGCGGACA GAACTACTCC ATGACCTTGT GCAGCACGTC 
AGTGACGTCG ACGTTGAGGA TGCGATGGGG CAGACAGCAC TGCATGTCGC CTGCCAGAAT 
GGTCACAAGA CGACAGTGCA GTGCTTGCTA GACAGTGGTG CTGATATTAA CAGGCCAAAT 
GTATCTGGAG CCACTCCATT GTACTTTGCT TGCAGTCATG GTCAGAGAGA CACAGCACAA 
ATTCTTCTGT TACGAGGAGC TAAATATCTG TCAGATAAAA ATGGAGTAAC GCCTCTGGAT 
TTATGTGTAC AGGGTGGCTA TGGAGAGACT TGTGAAGTAT TAATTCAGTA TCACCCTAGG 
CTTTTCCAGA CAATTATTCA AATGACACAG AATGAAGATC TCCGAGAAAA CATGTTACGG 
CAAGTTCTGG AACATTTGTC TCAGCAAAGT GAAAGCCAGT ACCTAAAGAT TCTAACAAGC 
CTTGCTGAAG TTGCCACAAC AAATGGGCAT AAACTGCTTA GTCTGTCTAG TAATTATGAT 
GCTCAGATGA AGAGCCTCTT AAGGATTGTG AGGATATTTT GTCATGTCTT TCGAATTGGT 
CCATCCTCTC CCAGTAATGG AATTGATTTG GGCTACAACG GGAATAAAAC TCCAAGAAGC 
CAAGTGTTCA AGCCTCTTGA ATTGCTTTGG CACTCATTAG ATGAATGGCT AGTTTTAATA 
GCTACAGAAT TGATGAAAAA CAAAAAGGAC TCAACGGATA TCACTTCTAT TTTACTGAAA 
CAAAAAGGCC AAGATCAAGA TGGTACTTCC ATTCCTCCAT TTGAACCTCC AGGACCTGGG 
AGCTATGAAA ATCTATCCAG TGGCACAGGA GAATCTAAAC CAGATGCTCT TGGAGGGAAA 
CAGGAAGCCA GTGCAGACTG TCAGGATGTT ATTTCTATGA CAGCTAACCG GCTAAGTGCT 
GTCATTCAAG CTTTTTACAT GTGCTGTTCT TGTCAGATGC CTCCAGGAAT GACCTCACCC 
CGTTTCATTG AATTTGTCTG CAAACATGAT GAAGTTTTAA AATGCTTTGT TAATAGAAAT 
CCCAAAATTA TATTTGATCA CTTTCATTTC CTCCTTGAAT GTCCTGAGTT GATGTCAAGA 
TTCATGCATA TCATAAAAGC ACAGCCATTT AAAGATCGCT GTGAGTGGTT CTATGAACAT 
TTGCACTCAG GACAACCAGA TTCTGACATG GTGCACAGGC CAGTGAATGA AAATGATATC 
CTGCTTGTTC ACAGAGATTC TATTTTTAGG AGTAGCTGTG AAGTTGTGTC AAAAGCAAAC 
TGTGCAAAGC TAAAGCAAGG AATTGCTGTA CGGTTCCATG GAGAAGAAGG CATGGGCCAA 
GGTGTTGTGC GTGAGTGGTT TGATATTCTG TCTAATGAGA TAGTGAATCC TGATTATGCA 
TTGTTCACCC AGTCAGCTGA TGGAACAACT TTTCAGCCTA ATAGTAATTC CTATGTAAAT 
CCTGATCACT TGAACTATTT CCGGTTTGCT GGACAGATCT TGGGATTAGC TTTGAACCAC 
AGGCAGCTGG TCAACATTTA CTTCACAAGA TCGTTCTATA AGCACATTCT TGGTATCCCT 
GTAAATTACC AAGATGTGGC ATCCATTGAT CCAGAATATG CCAAAAATTT GCAGTGGATT 
TTAGATAATG ACATTAGTGA TCTGGGTCTA GAACTAACCT TTTCTGTTGA AACTGATGTG 
TTTGGAGCAA TGGAAGAGGT GCCTTTGAAA CCTGGGGGTG GAAGTATTCT TGTGACACAA 
AATAACAAAG CGGAGTATGT CCAGCTTGTT ACTGAACTTC GAATGACAAG AGCCATTCAA 
CCTCAGATCA ATGCTTTTTT ACAGGGCTTT CATATGTTCA TTCCACCTTC CCTCATACAA 
CTTTTTGATG AATATGAATT GGAGCTACTG CTTTCTGGCA TGCCAGAAAT TGATGTGAGC 
GATTGGATAA GAAATACAGA ATACACAAGT GGCTATGAAA GAGAAGATCC AGTTATTCAG 
TGGTTCTGGG AAGTTGTGGA AGGCATTACT CCAGAGGAGA GAGTTCTTCT CTTGCAGTTT 
GTTACTGGCA GTTCCAGGGT CCCACATGGT GGATTTGCTA ACATCATGGG TGGAAGTGGA 
TTGCAAAACT TTACAATTGC TGCTGTGCCA TATACTCCAA ATCTCCTACC AACTTCAAGC 
ACATGCATCA ACATGCTCAA GTTACCTGAA TACCCAAGTA AAGAAATACT CAAGGACAGA 
CTTCTTGTGG CGTTACATTG TGGCAGCTAT GGTTACACAA TGGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( XM_022426401.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022282109.1 Canis lupus familiaris HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe292659
Clone ID Related Accession (Same CDS sequence)	XM_022426401.1 , XM_022426402.1
Accession Version	XM_022426401.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase HACE1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAAAT TATTAGAATA CAGTGCTGAT GTCAACATTT GTAATAATGA AGGCCTTACA 
GCAATACACT GGCTCGCTGT GAATGGGCGG ACAGAACTAC TCCATGACCT TGTGCAGCAC 
GTCAGTGACG TCGACGTTGA GGATGCGATG GGGCAGACAG CACTGCATGT CGCCTGCCAG 
AATGGTCACA AGACGACAGT GCAGTGCTTG CTAGACAGTG GTGCTGATAT TAACAGGCCA 
AATGTATCTG GAGCCACTCC ATTGTACTTT GCTTGCAGTC ATGGTCAGAG AGACACAGCA 
CAAATTCTTC TGTTACGAGG AGCTAAATAT CTGTCAGATA AAAATGGAGT AACGCCTCTG 
GATTTATGTG TACAGGGTGG CTATGGAGAG ACTTGTGAAG TATTAATTCA GTATCACCCT 
AGGCTTTTCC AGACAATTAT TCAAATGACA CAGAATGAAG ATCTCCGAGA AAACATGTTA 
CGGCAAGTTC TGGAACATTT GTCTCAGCAA AGTGAAAGCC AGTACCTAAA GATTCTAACA 
AGCCTTGCTG AAGTTGCCAC AACAAATGGG CATAAACTGC TTAGTCTGTC TAGTAATTAT 
GATGCTCAGA TGAAGAGCCT CTTAAGGATT GTGAGGATAT TTTGTCATGT CTTTCGAATT 
GGTCCATCCT CTCCCAGTAA TGGAATTGAT TTGGGCTACA ACGGGAATAA AACTCCAAGA 
AGCCAAGTGT TCAAGCCTCT TGAATTGCTT TGGCACTCAT TAGATGAATG GCTAGTTTTA 
ATAGCTACAG AATTGATGAA AAACAAAAAG GACTCAACGG ATATCACTTC TATTTTACTG 
AAACAAAAAG GCCAAGATCA AGATGGTACT TCCATTCCTC CATTTGAACC TCCAGGACCT 
GGGAGCTATG AAAATCTATC CAGTGGCACA GGAGAATCTA AACCAGATGC TCTTGGAGGG 
AAACAGGAAG CCAGTGCAGA CTGTCAGGAT GTTATTTCTA TGACAGCTAA CCGGCTAAGT 
GCTGTCATTC AAGCTTTTTA CATGTGCTGT TCTTGTCAGA TGCCTCCAGG AATGACCTCA 
CCCCGTTTCA TTGAATTTGT CTGCAAACAT GATGAAGTTT TAAAATGCTT TGTTAATAGA 
AATCCCAAAA TTATATTTGA TCACTTTCAT TTCCTCCTTG AATGTCCTGA GTTGATGTCA 
AGATTCATGC ATATCATAAA AGCACAGCCA TTTAAAGATC GCTGTGAGTG GTTCTATGAA 
CATTTGCACT CAGGACAACC AGATTCTGAC ATGGTGCACA GGCCAGTGAA TGAAAATGAT 
ATCCTGCTTG TTCACAGAGA TTCTATTTTT AGGAGTAGCT GTGAAGTTGT GTCAAAAGCA 
AACTGTGCAA AGCTAAAGCA AGGAATTGCT GTACGGTTCC ATGGAGAAGA AGGCATGGGC 
CAAGGTGTTG TGCGTGAGTG GTTTGATATT CTGTCTAATG AGATAGTGAA TCCTGATTAT 
GCATTGTTCA CCCAGTCAGC TGATGGAACA ACTTTTCAGC CTAATAGTAA TTCCTATGTA 
AATCCTGATC ACTTGAACTA TTTCCGGTTT GCTGGACAGA TCTTGGGATT AGCTTTGAAC 
CACAGGCAGC TGGTCAACAT TTACTTCACA AGATCGTTCT ATAAGCACAT TCTTGGTATC 
CCTGTAAATT ACCAAGATGT GGCATCCATT GATCCAGAAT ATGCCAAAAA TTTGCAGTGG 
ATTTTAGATA ATGACATTAG TGATCTGGGT CTAGAACTAA CCTTTTCTGT TGAAACTGAT 
GTGTTTGGAG CAATGGAAGA GGTGCCTTTG AAACCTGGGG GTGGAAGTAT TCTTGTGACA 
CAAAATAACA AAGCGGAGTA TGTCCAGCTT GTTACTGAAC TTCGAATGAC AAGAGCCATT 
CAACCTCAGA TCAATGCTTT TTTACAGGGC TTTCATATGT TCATTCCACC TTCCCTCATA 
CAACTTTTTG ATGAATATGA ATTGGAGCTA CTGCTTTCTG GCATGCCAGA AATTGATGTG 
AGCGATTGGA TAAGAAATAC AGAATACACA AGTGGCTATG AAAGAGAAGA TCCAGTTATT 
CAGTGGTTCT GGGAAGTTGT GGAAGGCATT ACTCCAGAGG AGAGAGTTCT TCTCTTGCAG 
TTTGTTACTG GCAGTTCCAG GGTCCCACAT GGTGGATTTG CTAACATCAT GGGTGGAAGT 
GGATTGCAAA ACTTTACAAT TGCTGCTGTG CCATATACTC CAAATCTCCT ACCAACTTCA 
AGCACATGCA TCAACATGCT CAAGTTACCT GAATACCCAA GTAAAGAAAT ACTCAAGGAC 
AGACTTCTTG TGGCGTTACA TTGTGGCAGC TATGGTTACA CAATGGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022282109.1
CDS	231..2621
Translation	MSKLLEYSADVNICNNEGLTAIHWLAVNGRTELLHDLVQHVSDVDVEDAMGQTALHVACQNGHKTTVQCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLLRGAKYLSDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRIFCHVFRIGPSSPSNGIDLGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKKDSTDITSILLKQKGQDQDGTSIPPFEPPGPGSYENLSSGTGESKPDALGGKQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIRNTEYTSGYEREDPVIQWFWEVVEGITPEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	XM_022426401.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022426401.1

ORF Insert Sequence:

ATGAGTAAAT TATTAGAATA CAGTGCTGAT GTCAACATTT GTAATAATGA AGGCCTTACA 
GCAATACACT GGCTCGCTGT GAATGGGCGG ACAGAACTAC TCCATGACCT TGTGCAGCAC 
GTCAGTGACG TCGACGTTGA GGATGCGATG GGGCAGACAG CACTGCATGT CGCCTGCCAG 
AATGGTCACA AGACGACAGT GCAGTGCTTG CTAGACAGTG GTGCTGATAT TAACAGGCCA 
AATGTATCTG GAGCCACTCC ATTGTACTTT GCTTGCAGTC ATGGTCAGAG AGACACAGCA 
CAAATTCTTC TGTTACGAGG AGCTAAATAT CTGTCAGATA AAAATGGAGT AACGCCTCTG 
GATTTATGTG TACAGGGTGG CTATGGAGAG ACTTGTGAAG TATTAATTCA GTATCACCCT 
AGGCTTTTCC AGACAATTAT TCAAATGACA CAGAATGAAG ATCTCCGAGA AAACATGTTA 
CGGCAAGTTC TGGAACATTT GTCTCAGCAA AGTGAAAGCC AGTACCTAAA GATTCTAACA 
AGCCTTGCTG AAGTTGCCAC AACAAATGGG CATAAACTGC TTAGTCTGTC TAGTAATTAT 
GATGCTCAGA TGAAGAGCCT CTTAAGGATT GTGAGGATAT TTTGTCATGT CTTTCGAATT 
GGTCCATCCT CTCCCAGTAA TGGAATTGAT TTGGGCTACA ACGGGAATAA AACTCCAAGA 
AGCCAAGTGT TCAAGCCTCT TGAATTGCTT TGGCACTCAT TAGATGAATG GCTAGTTTTA 
ATAGCTACAG AATTGATGAA AAACAAAAAG GACTCAACGG ATATCACTTC TATTTTACTG 
AAACAAAAAG GCCAAGATCA AGATGGTACT TCCATTCCTC CATTTGAACC TCCAGGACCT 
GGGAGCTATG AAAATCTATC CAGTGGCACA GGAGAATCTA AACCAGATGC TCTTGGAGGG 
AAACAGGAAG CCAGTGCAGA CTGTCAGGAT GTTATTTCTA TGACAGCTAA CCGGCTAAGT 
GCTGTCATTC AAGCTTTTTA CATGTGCTGT TCTTGTCAGA TGCCTCCAGG AATGACCTCA 
CCCCGTTTCA TTGAATTTGT CTGCAAACAT GATGAAGTTT TAAAATGCTT TGTTAATAGA 
AATCCCAAAA TTATATTTGA TCACTTTCAT TTCCTCCTTG AATGTCCTGA GTTGATGTCA 
AGATTCATGC ATATCATAAA AGCACAGCCA TTTAAAGATC GCTGTGAGTG GTTCTATGAA 
CATTTGCACT CAGGACAACC AGATTCTGAC ATGGTGCACA GGCCAGTGAA TGAAAATGAT 
ATCCTGCTTG TTCACAGAGA TTCTATTTTT AGGAGTAGCT GTGAAGTTGT GTCAAAAGCA 
AACTGTGCAA AGCTAAAGCA AGGAATTGCT GTACGGTTCC ATGGAGAAGA AGGCATGGGC 
CAAGGTGTTG TGCGTGAGTG GTTTGATATT CTGTCTAATG AGATAGTGAA TCCTGATTAT 
GCATTGTTCA CCCAGTCAGC TGATGGAACA ACTTTTCAGC CTAATAGTAA TTCCTATGTA 
AATCCTGATC ACTTGAACTA TTTCCGGTTT GCTGGACAGA TCTTGGGATT AGCTTTGAAC 
CACAGGCAGC TGGTCAACAT TTACTTCACA AGATCGTTCT ATAAGCACAT TCTTGGTATC 
CCTGTAAATT ACCAAGATGT GGCATCCATT GATCCAGAAT ATGCCAAAAA TTTGCAGTGG 
ATTTTAGATA ATGACATTAG TGATCTGGGT CTAGAACTAA CCTTTTCTGT TGAAACTGAT 
GTGTTTGGAG CAATGGAAGA GGTGCCTTTG AAACCTGGGG GTGGAAGTAT TCTTGTGACA 
CAAAATAACA AAGCGGAGTA TGTCCAGCTT GTTACTGAAC TTCGAATGAC AAGAGCCATT 
CAACCTCAGA TCAATGCTTT TTTACAGGGC TTTCATATGT TCATTCCACC TTCCCTCATA 
CAACTTTTTG ATGAATATGA ATTGGAGCTA CTGCTTTCTG GCATGCCAGA AATTGATGTG 
AGCGATTGGA TAAGAAATAC AGAATACACA AGTGGCTATG AAAGAGAAGA TCCAGTTATT 
CAGTGGTTCT GGGAAGTTGT GGAAGGCATT ACTCCAGAGG AGAGAGTTCT TCTCTTGCAG 
TTTGTTACTG GCAGTTCCAG GGTCCCACAT GGTGGATTTG CTAACATCAT GGGTGGAAGT 
GGATTGCAAA ACTTTACAAT TGCTGCTGTG CCATATACTC CAAATCTCCT ACCAACTTCA 
AGCACATGCA TCAACATGCT CAAGTTACCT GAATACCCAA GTAAAGAAAT ACTCAAGGAC 
AGACTTCTTG TGGCGTTACA TTGTGGCAGC TATGGTTACA CAATGGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( XM_022426402.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022282110.1 Canis lupus familiaris HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe292659
Clone ID Related Accession (Same CDS sequence)	XM_022426401.1 , XM_022426402.1
Accession Version	XM_022426402.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase HACE1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAAAT TATTAGAATA CAGTGCTGAT GTCAACATTT GTAATAATGA AGGCCTTACA 
GCAATACACT GGCTCGCTGT GAATGGGCGG ACAGAACTAC TCCATGACCT TGTGCAGCAC 
GTCAGTGACG TCGACGTTGA GGATGCGATG GGGCAGACAG CACTGCATGT CGCCTGCCAG 
AATGGTCACA AGACGACAGT GCAGTGCTTG CTAGACAGTG GTGCTGATAT TAACAGGCCA 
AATGTATCTG GAGCCACTCC ATTGTACTTT GCTTGCAGTC ATGGTCAGAG AGACACAGCA 
CAAATTCTTC TGTTACGAGG AGCTAAATAT CTGTCAGATA AAAATGGAGT AACGCCTCTG 
GATTTATGTG TACAGGGTGG CTATGGAGAG ACTTGTGAAG TATTAATTCA GTATCACCCT 
AGGCTTTTCC AGACAATTAT TCAAATGACA CAGAATGAAG ATCTCCGAGA AAACATGTTA 
CGGCAAGTTC TGGAACATTT GTCTCAGCAA AGTGAAAGCC AGTACCTAAA GATTCTAACA 
AGCCTTGCTG AAGTTGCCAC AACAAATGGG CATAAACTGC TTAGTCTGTC TAGTAATTAT 
GATGCTCAGA TGAAGAGCCT CTTAAGGATT GTGAGGATAT TTTGTCATGT CTTTCGAATT 
GGTCCATCCT CTCCCAGTAA TGGAATTGAT TTGGGCTACA ACGGGAATAA AACTCCAAGA 
AGCCAAGTGT TCAAGCCTCT TGAATTGCTT TGGCACTCAT TAGATGAATG GCTAGTTTTA 
ATAGCTACAG AATTGATGAA AAACAAAAAG GACTCAACGG ATATCACTTC TATTTTACTG 
AAACAAAAAG GCCAAGATCA AGATGGTACT TCCATTCCTC CATTTGAACC TCCAGGACCT 
GGGAGCTATG AAAATCTATC CAGTGGCACA GGAGAATCTA AACCAGATGC TCTTGGAGGG 
AAACAGGAAG CCAGTGCAGA CTGTCAGGAT GTTATTTCTA TGACAGCTAA CCGGCTAAGT 
GCTGTCATTC AAGCTTTTTA CATGTGCTGT TCTTGTCAGA TGCCTCCAGG AATGACCTCA 
CCCCGTTTCA TTGAATTTGT CTGCAAACAT GATGAAGTTT TAAAATGCTT TGTTAATAGA 
AATCCCAAAA TTATATTTGA TCACTTTCAT TTCCTCCTTG AATGTCCTGA GTTGATGTCA 
AGATTCATGC ATATCATAAA AGCACAGCCA TTTAAAGATC GCTGTGAGTG GTTCTATGAA 
CATTTGCACT CAGGACAACC AGATTCTGAC ATGGTGCACA GGCCAGTGAA TGAAAATGAT 
ATCCTGCTTG TTCACAGAGA TTCTATTTTT AGGAGTAGCT GTGAAGTTGT GTCAAAAGCA 
AACTGTGCAA AGCTAAAGCA AGGAATTGCT GTACGGTTCC ATGGAGAAGA AGGCATGGGC 
CAAGGTGTTG TGCGTGAGTG GTTTGATATT CTGTCTAATG AGATAGTGAA TCCTGATTAT 
GCATTGTTCA CCCAGTCAGC TGATGGAACA ACTTTTCAGC CTAATAGTAA TTCCTATGTA 
AATCCTGATC ACTTGAACTA TTTCCGGTTT GCTGGACAGA TCTTGGGATT AGCTTTGAAC 
CACAGGCAGC TGGTCAACAT TTACTTCACA AGATCGTTCT ATAAGCACAT TCTTGGTATC 
CCTGTAAATT ACCAAGATGT GGCATCCATT GATCCAGAAT ATGCCAAAAA TTTGCAGTGG 
ATTTTAGATA ATGACATTAG TGATCTGGGT CTAGAACTAA CCTTTTCTGT TGAAACTGAT 
GTGTTTGGAG CAATGGAAGA GGTGCCTTTG AAACCTGGGG GTGGAAGTAT TCTTGTGACA 
CAAAATAACA AAGCGGAGTA TGTCCAGCTT GTTACTGAAC TTCGAATGAC AAGAGCCATT 
CAACCTCAGA TCAATGCTTT TTTACAGGGC TTTCATATGT TCATTCCACC TTCCCTCATA 
CAACTTTTTG ATGAATATGA ATTGGAGCTA CTGCTTTCTG GCATGCCAGA AATTGATGTG 
AGCGATTGGA TAAGAAATAC AGAATACACA AGTGGCTATG AAAGAGAAGA TCCAGTTATT 
CAGTGGTTCT GGGAAGTTGT GGAAGGCATT ACTCCAGAGG AGAGAGTTCT TCTCTTGCAG 
TTTGTTACTG GCAGTTCCAG GGTCCCACAT GGTGGATTTG CTAACATCAT GGGTGGAAGT 
GGATTGCAAA ACTTTACAAT TGCTGCTGTG CCATATACTC CAAATCTCCT ACCAACTTCA 
AGCACATGCA TCAACATGCT CAAGTTACCT GAATACCCAA GTAAAGAAAT ACTCAAGGAC 
AGACTTCTTG TGGCGTTACA TTGTGGCAGC TATGGTTACA CAATGGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022282110.1
CDS	138..2528
Translation	MSKLLEYSADVNICNNEGLTAIHWLAVNGRTELLHDLVQHVSDVDVEDAMGQTALHVACQNGHKTTVQCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLLRGAKYLSDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRIFCHVFRIGPSSPSNGIDLGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKKDSTDITSILLKQKGQDQDGTSIPPFEPPGPGSYENLSSGTGESKPDALGGKQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIRNTEYTSGYEREDPVIQWFWEVVEGITPEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	XM_022426402.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022426402.1

ORF Insert Sequence:

ATGAGTAAAT TATTAGAATA CAGTGCTGAT GTCAACATTT GTAATAATGA AGGCCTTACA 
GCAATACACT GGCTCGCTGT GAATGGGCGG ACAGAACTAC TCCATGACCT TGTGCAGCAC 
GTCAGTGACG TCGACGTTGA GGATGCGATG GGGCAGACAG CACTGCATGT CGCCTGCCAG 
AATGGTCACA AGACGACAGT GCAGTGCTTG CTAGACAGTG GTGCTGATAT TAACAGGCCA 
AATGTATCTG GAGCCACTCC ATTGTACTTT GCTTGCAGTC ATGGTCAGAG AGACACAGCA 
CAAATTCTTC TGTTACGAGG AGCTAAATAT CTGTCAGATA AAAATGGAGT AACGCCTCTG 
GATTTATGTG TACAGGGTGG CTATGGAGAG ACTTGTGAAG TATTAATTCA GTATCACCCT 
AGGCTTTTCC AGACAATTAT TCAAATGACA CAGAATGAAG ATCTCCGAGA AAACATGTTA 
CGGCAAGTTC TGGAACATTT GTCTCAGCAA AGTGAAAGCC AGTACCTAAA GATTCTAACA 
AGCCTTGCTG AAGTTGCCAC AACAAATGGG CATAAACTGC TTAGTCTGTC TAGTAATTAT 
GATGCTCAGA TGAAGAGCCT CTTAAGGATT GTGAGGATAT TTTGTCATGT CTTTCGAATT 
GGTCCATCCT CTCCCAGTAA TGGAATTGAT TTGGGCTACA ACGGGAATAA AACTCCAAGA 
AGCCAAGTGT TCAAGCCTCT TGAATTGCTT TGGCACTCAT TAGATGAATG GCTAGTTTTA 
ATAGCTACAG AATTGATGAA AAACAAAAAG GACTCAACGG ATATCACTTC TATTTTACTG 
AAACAAAAAG GCCAAGATCA AGATGGTACT TCCATTCCTC CATTTGAACC TCCAGGACCT 
GGGAGCTATG AAAATCTATC CAGTGGCACA GGAGAATCTA AACCAGATGC TCTTGGAGGG 
AAACAGGAAG CCAGTGCAGA CTGTCAGGAT GTTATTTCTA TGACAGCTAA CCGGCTAAGT 
GCTGTCATTC AAGCTTTTTA CATGTGCTGT TCTTGTCAGA TGCCTCCAGG AATGACCTCA 
CCCCGTTTCA TTGAATTTGT CTGCAAACAT GATGAAGTTT TAAAATGCTT TGTTAATAGA 
AATCCCAAAA TTATATTTGA TCACTTTCAT TTCCTCCTTG AATGTCCTGA GTTGATGTCA 
AGATTCATGC ATATCATAAA AGCACAGCCA TTTAAAGATC GCTGTGAGTG GTTCTATGAA 
CATTTGCACT CAGGACAACC AGATTCTGAC ATGGTGCACA GGCCAGTGAA TGAAAATGAT 
ATCCTGCTTG TTCACAGAGA TTCTATTTTT AGGAGTAGCT GTGAAGTTGT GTCAAAAGCA 
AACTGTGCAA AGCTAAAGCA AGGAATTGCT GTACGGTTCC ATGGAGAAGA AGGCATGGGC 
CAAGGTGTTG TGCGTGAGTG GTTTGATATT CTGTCTAATG AGATAGTGAA TCCTGATTAT 
GCATTGTTCA CCCAGTCAGC TGATGGAACA ACTTTTCAGC CTAATAGTAA TTCCTATGTA 
AATCCTGATC ACTTGAACTA TTTCCGGTTT GCTGGACAGA TCTTGGGATT AGCTTTGAAC 
CACAGGCAGC TGGTCAACAT TTACTTCACA AGATCGTTCT ATAAGCACAT TCTTGGTATC 
CCTGTAAATT ACCAAGATGT GGCATCCATT GATCCAGAAT ATGCCAAAAA TTTGCAGTGG 
ATTTTAGATA ATGACATTAG TGATCTGGGT CTAGAACTAA CCTTTTCTGT TGAAACTGAT 
GTGTTTGGAG CAATGGAAGA GGTGCCTTTG AAACCTGGGG GTGGAAGTAT TCTTGTGACA 
CAAAATAACA AAGCGGAGTA TGTCCAGCTT GTTACTGAAC TTCGAATGAC AAGAGCCATT 
CAACCTCAGA TCAATGCTTT TTTACAGGGC TTTCATATGT TCATTCCACC TTCCCTCATA 
CAACTTTTTG ATGAATATGA ATTGGAGCTA CTGCTTTCTG GCATGCCAGA AATTGATGTG 
AGCGATTGGA TAAGAAATAC AGAATACACA AGTGGCTATG AAAGAGAAGA TCCAGTTATT 
CAGTGGTTCT GGGAAGTTGT GGAAGGCATT ACTCCAGAGG AGAGAGTTCT TCTCTTGCAG 
TTTGTTACTG GCAGTTCCAG GGTCCCACAT GGTGGATTTG CTAACATCAT GGGTGGAAGT 
GGATTGCAAA ACTTTACAAT TGCTGCTGTG CCATATACTC CAAATCTCCT ACCAACTTCA 
AGCACATGCA TCAACATGCT CAAGTTACCT GAATACCCAA GTAAAGAAAT ACTCAAGGAC 
AGACTTCTTG TGGCGTTACA TTGTGGCAGC TATGGTTACA CAATGGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACE1 ( XM_022426403.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022282111.1 Canis lupus familiaris HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe292660
Clone ID Related Accession (Same CDS sequence)	XM_022426403.1
Accession Version	XM_022426403.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase HACE1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGAC AGAACTACTC CATGACCTTG TGCAGCACGT CAGTGACGTC GACGTTGAGG 
ATGCGATGGG GCAGACAGCA CTGCATGTCG CCTGCCAGAA TGGTCACAAG ACGTCATGGT 
CAGAGAGACA CAGCACAAAT TCTTCTGTTA CGAGGAGCTA AATATCTGTC AGATAAAAAT 
GGAGTAACGC CTCTGGATTT ATGTGTACAG GGTGGCTATG GAGAGACTTG TGAAGTATTA 
ATTCAGTATC ACCCTAGGCT TTTCCAGACA ATTATTCAAA TGACACAGAA TGAAGATCTC 
CGAGAAAACA TGTTACGGCA AGTTCTGGAA CATTTGTCTC AGCAAAGTGA AAGCCAGTAC 
CTAAAGATTC TAACAAGCCT TGCTGAAGTT GCCACAACAA ATGGGCATAA ACTGCTTAGT 
CTGTCTAGTA ATTATGATGC TCAGATGAAG AGCCTCTTAA GGATTGTGAG GATATTTTGT 
CATGTCTTTC GAATTGGTCC ATCCTCTCCC AGTAATGGAA TTGATTTGGG CTACAACGGG 
AATAAAACTC CAAGAAGCCA AGTGTTCAAG CCTCTTGAAT TGCTTTGGCA CTCATTAGAT 
GAATGGCTAG TTTTAATAGC TACAGAATTG ATGAAAAACA AAAAGGACTC AACGGATATC 
ACTTCTATTT TACTGAAACA AAAAGGCCAA GATCAAGATG GTACTTCCAT TCCTCCATTT 
GAACCTCCAG GACCTGGGAG CTATGAAAAT CTATCCAGTG GCACAGGAGA ATCTAAACCA 
GATGCTCTTG GAGGGAAACA GGAAGCCAGT GCAGACTGTC AGGATGTTAT TTCTATGACA 
GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT 
CCAGGAATGA CCTCACCCCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA 
TGCTTTGTTA ATAGAAATCC CAAAATTATA TTTGATCACT TTCATTTCCT CCTTGAATGT 
CCTGAGTTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCATTTAA AGATCGCTGT 
GAGTGGTTCT ATGAACATTT GCACTCAGGA CAACCAGATT CTGACATGGT GCACAGGCCA 
GTGAATGAAA ATGATATCCT GCTTGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA 
GTTGTGTCAA AAGCAAACTG TGCAAAGCTA AAGCAAGGAA TTGCTGTACG GTTCCATGGA 
GAAGAAGGCA TGGGCCAAGG TGTTGTGCGT GAGTGGTTTG ATATTCTGTC TAATGAGATA 
GTGAATCCTG ATTATGCATT GTTCACCCAG TCAGCTGATG GAACAACTTT TCAGCCTAAT 
AGTAATTCCT ATGTAAATCC TGATCACTTG AACTATTTCC GGTTTGCTGG ACAGATCTTG 
GGATTAGCTT TGAACCACAG GCAGCTGGTC AACATTTACT TCACAAGATC GTTCTATAAG 
CACATTCTTG GTATCCCTGT AAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCC 
AAAAATTTGC AGTGGATTTT AGATAATGAC ATTAGTGATC TGGGTCTAGA ACTAACCTTT 
TCTGTTGAAA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CTTTGAAACC TGGGGGTGGA 
AGTATTCTTG TGACACAAAA TAACAAAGCG GAGTATGTCC AGCTTGTTAC TGAACTTCGA 
ATGACAAGAG CCATTCAACC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT 
CCACCTTCCC TCATACAACT TTTTGATGAA TATGAATTGG AGCTACTGCT TTCTGGCATG 
CCAGAAATTG ATGTGAGCGA TTGGATAAGA AATACAGAAT ACACAAGTGG CTATGAAAGA 
GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTGGAAG GCATTACTCC AGAGGAGAGA 
GTTCTTCTCT TGCAGTTTGT TACTGGCAGT TCCAGGGTCC CACATGGTGG ATTTGCTAAC 
ATCATGGGTG GAAGTGGATT GCAAAACTTT ACAATTGCTG CTGTGCCATA TACTCCAAAT 
CTCCTACCAA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA 
GAAATACTCA AGGACAGACT TCTTGTGGCG TTACATTGTG GCAGCTATGG TTACACAATG 
GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022282111.1
CDS	1..2226
Translation	MGGQNYSMTLCSTSVTSTLRMRWGRQHCMSPARMVTRRHGQRDTAQILLLRGAKYLSDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRIFCHVFRIGPSSPSNGIDLGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKKDSTDITSILLKQKGQDQDGTSIPPFEPPGPGSYENLSSGTGESKPDALGGKQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIRNTEYTSGYEREDPVIQWFWEVVEGITPEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

Target ORF information:

RefSeq Version	XM_022426403.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022426403.1

ORF Insert Sequence:

ATGGGCGGAC AGAACTACTC CATGACCTTG TGCAGCACGT CAGTGACGTC GACGTTGAGG 
ATGCGATGGG GCAGACAGCA CTGCATGTCG CCTGCCAGAA TGGTCACAAG ACGTCATGGT 
CAGAGAGACA CAGCACAAAT TCTTCTGTTA CGAGGAGCTA AATATCTGTC AGATAAAAAT 
GGAGTAACGC CTCTGGATTT ATGTGTACAG GGTGGCTATG GAGAGACTTG TGAAGTATTA 
ATTCAGTATC ACCCTAGGCT TTTCCAGACA ATTATTCAAA TGACACAGAA TGAAGATCTC 
CGAGAAAACA TGTTACGGCA AGTTCTGGAA CATTTGTCTC AGCAAAGTGA AAGCCAGTAC 
CTAAAGATTC TAACAAGCCT TGCTGAAGTT GCCACAACAA ATGGGCATAA ACTGCTTAGT 
CTGTCTAGTA ATTATGATGC TCAGATGAAG AGCCTCTTAA GGATTGTGAG GATATTTTGT 
CATGTCTTTC GAATTGGTCC ATCCTCTCCC AGTAATGGAA TTGATTTGGG CTACAACGGG 
AATAAAACTC CAAGAAGCCA AGTGTTCAAG CCTCTTGAAT TGCTTTGGCA CTCATTAGAT 
GAATGGCTAG TTTTAATAGC TACAGAATTG ATGAAAAACA AAAAGGACTC AACGGATATC 
ACTTCTATTT TACTGAAACA AAAAGGCCAA GATCAAGATG GTACTTCCAT TCCTCCATTT 
GAACCTCCAG GACCTGGGAG CTATGAAAAT CTATCCAGTG GCACAGGAGA ATCTAAACCA 
GATGCTCTTG GAGGGAAACA GGAAGCCAGT GCAGACTGTC AGGATGTTAT TTCTATGACA 
GCTAACCGGC TAAGTGCTGT CATTCAAGCT TTTTACATGT GCTGTTCTTG TCAGATGCCT 
CCAGGAATGA CCTCACCCCG TTTCATTGAA TTTGTCTGCA AACATGATGA AGTTTTAAAA 
TGCTTTGTTA ATAGAAATCC CAAAATTATA TTTGATCACT TTCATTTCCT CCTTGAATGT 
CCTGAGTTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCATTTAA AGATCGCTGT 
GAGTGGTTCT ATGAACATTT GCACTCAGGA CAACCAGATT CTGACATGGT GCACAGGCCA 
GTGAATGAAA ATGATATCCT GCTTGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA 
GTTGTGTCAA AAGCAAACTG TGCAAAGCTA AAGCAAGGAA TTGCTGTACG GTTCCATGGA 
GAAGAAGGCA TGGGCCAAGG TGTTGTGCGT GAGTGGTTTG ATATTCTGTC TAATGAGATA 
GTGAATCCTG ATTATGCATT GTTCACCCAG TCAGCTGATG GAACAACTTT TCAGCCTAAT 
AGTAATTCCT ATGTAAATCC TGATCACTTG AACTATTTCC GGTTTGCTGG ACAGATCTTG 
GGATTAGCTT TGAACCACAG GCAGCTGGTC AACATTTACT TCACAAGATC GTTCTATAAG 
CACATTCTTG GTATCCCTGT AAATTACCAA GATGTGGCAT CCATTGATCC AGAATATGCC 
AAAAATTTGC AGTGGATTTT AGATAATGAC ATTAGTGATC TGGGTCTAGA ACTAACCTTT 
TCTGTTGAAA CTGATGTGTT TGGAGCAATG GAAGAGGTGC CTTTGAAACC TGGGGGTGGA 
AGTATTCTTG TGACACAAAA TAACAAAGCG GAGTATGTCC AGCTTGTTAC TGAACTTCGA 
ATGACAAGAG CCATTCAACC TCAGATCAAT GCTTTTTTAC AGGGCTTTCA TATGTTCATT 
CCACCTTCCC TCATACAACT TTTTGATGAA TATGAATTGG AGCTACTGCT TTCTGGCATG 
CCAGAAATTG ATGTGAGCGA TTGGATAAGA AATACAGAAT ACACAAGTGG CTATGAAAGA 
GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTGGAAG GCATTACTCC AGAGGAGAGA 
GTTCTTCTCT TGCAGTTTGT TACTGGCAGT TCCAGGGTCC CACATGGTGG ATTTGCTAAC 
ATCATGGGTG GAAGTGGATT GCAAAACTTT ACAATTGCTG CTGTGCCATA TACTCCAAAT 
CTCCTACCAA CTTCAAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA 
GAAATACTCA AGGACAGACT TCTTGTGGCG TTACATTGTG GCAGCTATGG TTACACAATG 
GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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HACE1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

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