ADGRF5 cDNA ORF clone, Canis lupus familiaris(Dog)

The following ADGRF5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ADGRF5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe15371 XM_005627441.3
Latest version!
Canis lupus familiaris adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OCe15371 XM_005627442.3
Latest version!
Canis lupus familiaris adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OCe15371 XM_022426127.1
Latest version!
Canis lupus familiaris adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OCe15372 XM_005627443.3
Latest version!
Canis lupus familiaris adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OCe15373 XM_005627444.3
Latest version!
Canis lupus familiaris adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OCe15374 XM_005627445.3
Latest version!
Canis lupus familiaris adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OCe15375 XM_532160.6
Latest version!
Canis lupus familiaris adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
View Old Accession Versions >>
OCe15373 XM_005627444.2 Canis lupus familiaris adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OCe15371 XM_005627442.2 Canis lupus familiaris adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OCe15372 XM_005627443.2 Canis lupus familiaris adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OCe15371 XM_005627441.2 Canis lupus familiaris adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OCe15374 XM_005627445.2 Canis lupus familiaris adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OCe15375 XM_532160.5 Canis lupus familiaris adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe15371
    Clone ID Related Accession (Same CDS sequence) XM_005627442.2 , XM_005627441.3 , XM_022426127.1 , XM_005627442.3 , XM_005627441.2
    Accession Version XM_005627442.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4167bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product adhesion G protein-coupled receptor F5 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005627442.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAATCCC CAAGGAGAAC CACATTGTGC TTCATGCTTA TTGTGATTTA TTTTTCCCAA 
    GCTACACTGA ACTTGAATTT AGAATCTATT GTTCATCCTT TGATTCTCCA TGAACATGAA
    CTAACAGAGA AGGAGCCACT AAGGGAAAAA CGAGCAGTTG CCTCCAGCAG CCCAGTACCT
    GCAGAGTACA CTGTTGATGT GGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAGCCTATC
    AAAGCTTATG TGAACAGCCT CAGTTTCCCA ATTCCAGGGA ACAGCACAGA CCTGGCCACC
    AACATTTTAA GCATTGAGGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CGGGCTATAG GTGGCCTCAG GAGAAGTGCC TCCACAATCC CACTTGTCAA
    GAGCATGACA GCTCCCTTGC AGGGCATCGT TGCAGTTGCC TGAAAGGACT ACCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGCAGAT GTTACTTTAA GAATGTCAGT CAGACTCAAT
    GTGGGCTTTC AAGAGGACCT CAAGAATTCT TCCTCTGCTC TCTATAGGTC CTACAAGACT
    GACCTGGAAA CAGCGTTCTG GAAAGGTTAC AGTACTTTAC CAGGCTTCAA ATTGGTGACT
    GTGACAGGGT TCAGGCCCGG AAGTGTGGTT GTGACATATG AGGTGAAGAC TACAACACCA
    TCACCTCAAT TGATGCATAA AGCAAATGAG CAAGTGATGC AGAACCTGAA TCAGACCTAC
    AGAATGGACT ACAGTTCCTT TCAAGCCGTT ACTAGCAATG AAACTAAGTT CACCATCATA
    CCAGAAATCA TTTTTGAAGG GGATACGGTA AACATGATGT GTGAAACCGA CGTTTTGTCC
    TCTAACGTGT CCTGGCACCA TGTTGAAAGG CACTCTGACA TCCAGAACAG CAGCAGATTC
    TCAGTTTACA CCACCGTGCT GAACAACATG ACCTCAGTCT CCCGCCTCAC GATTTACAAC
    ATCACCCAGC GGGATGCAGG TGAATATATT TGCAAGCTGA CATTAGATAT TTTTGAATAT
    GAGTCCAGAA AAAAAATAGA TGTCACGCCC ATCCGAATTC TGGCAACTGA AGAAATGAAG
    GTGACGTGCG ACAACAATCC TATATCTTTG AACTGCTGCA GTGAGATTAA TGCTAACTGG
    AGCACAATAG AATGGAAGCA GGAGGGGAAA ATAAACATTC CAGGCAACCC TGAAACTGAC
    CTAGAGTCCA GCTGCAGCAG ATACACCCTC AAGGCAGATG GAACCCAGTG TCCGAGTGGG
    TCGGCTGGAA CCACGGGCAT CTTTGTGTGT GAGTTCATCA GCACCTACGG AGCCAGAGGC
    AGTAAGAGCA TAGAAGTGAC GTTCATCTCT GTGGGCGATA ATTCCAGCCA CAAAGGGGAA
    GTGCACGAGA TCCTCAGGCA TGATACGGAA ATGAGTACAG TTCAGAGCAC CACCGGGAAC
    AGCCTAGAAA CAAGTAAAGC CCAGGAGCAG CAAGCCAACC TAACAATAAC CCCGGACCCA
    ATTTCTGTTT CTGAGGGACA AAACTTTTCC ATAAAGTGCA TCAGCGATGT GAGTAACTAT
    GATGAGGTAT ACTGGAACAC GTCTGCTGGA ATTAAAATAT ACCAAAAGTT TTATACCACG
    AGGAGGTTTT CTGATGGAGC AGAGTCAGTG CTGACAGTGA AGACTGTGAC CCGGGAGTGG
    AACGGAACCT ATCACTGCAT ATTTAGATAC AAGAATTCAT ACAGTGTCGT AATGAAAGAT
    GTGACCGTTT ACCCACTGCC TCTAGAGCCA AACATCATGG TTGATCCTCT GGAGGCTACA
    GTTCCCTGCA AGGGTTCCCA TCACTTCAGA TGCTGCATTG ACGAGGATGA GAACTACAGA
    GTGACTTTCC AAGTGGATTC CTTATCCTTT CCTGCAGAAA AAGAAGTTAA TGGCAAGCAA
    GTGTGCTACA AATACAAGTT CATGGCAAGG ACATCAGTTT CCTGGTGTCT AAAAAAGCTG
    GACATATTTT GTCACTTTAC CAATGCTGCT AATAATTCAG TCCGGAGCCC ATCTATGAAG
    CTTAATGTGG TTCCTGGAAA GAACATCATG TGCCGAGATC CCGTAATAGG TGTTGGGGAG
    CCTGGAAAAG TCATCCAGAA ACTCTGCCAT TTCTCAAAAG TACCAAACAT CTCTGGAGGT
    CCTACTGGAG GGATCATAAC TTACAAATGT GTAGGCTCCC AATGGGAGGT GAAGAAAAAT
    GACTGCATCT CTGCCCCAAT AAATAGTCTG CTCCAGCTGG CCAAGGCTTT GACCAAGAGC
    CCCACTCAGG ATAAGAAGCT CCCAACATAC CTAAAGGATC TTTCTATTAG CACAGGCAAG
    GTAAAACATG AAGTCAGCTC ATTTCCTGGG AGCCTGGGAG CCATCGTTAA CATCCTTGAT
    TTGCTTTCAA CAGTTACAAC CCAAGTGAAT TCAGAGATGA TGATGAACGT TCTCTCTACG
    GTTAACACTG TCCTCAGCAA GCCCATGTTG AGTACCTGGA AGGTGTCCCA ACAGCAACAG
    ACCAACCAGA GCTCACAGCT ACTGGATTCA GTAGAAAGAT TTTCCCGAGC ATTACAGACA
    GAAGATAGCA CCTTTTTATC CATCAACCAA ACTAACGTGC AGATGAGGAG CATGGTAATA
    AAAACTGGCC ATCCAAAAAC CTACAAACAG AGCTTTGTTT TCTCAGACTT GGACCTCTGG
    GGCAATGTGG CCATTGACAA GTGCGAGGTG GGAAGCCTGC AGCAAGATTC ATCTATTGTC
    ACCGTGGCTT TCCCAACTCT CCAAACCATC CTCACTCAGG ATGTTCAAGG AAAGAATTTT
    GCCAACAGCT TAGTGATGAC AACCACTGTT AGCCACAACA TAACCATGCC ATTTAGGATT
    TCAATGACTT TTAAGAACAT CAATCCTTCA GGTGGGGTAC CACAATGTGT CTACTGGAAT
    TTCAACCTCG CCAACCACAC AGGGGGTTGG GATAGTAGTG GGTGTTATGT GCACAATGTC
    GATGGGGACA GTGTCTCCTG CATCTGTGAC CACCTAACAT CATTCTCCAT CCTCATGTCC
    CCCGACTCTC CGTACCCTGG TTCCCTCCTG GGAATACTAC TGGATATCAT TTCCTACATT
    GGGTTATGCT TTTCCATCTT GAGCTTAGCA GCCTGTCTAG TGGTGGAAGC TGTGGTGTGG
    AAATCAGTGA CCAAGAATCG GACTTCCTAT ATGCGCCACA CCTGCATAGT GAACATTGCA
    GCCTCTCTTC TGGTTGCTGA CATCTGGTTC ATTGTAGCTG CTGCCATCCA CGACCATTTT
    TACCCACTCA ATGAGACAGC CTGTGTGGCC GCCACCTTTT TCATCCACTT CTTCTACCTC
    AGCGTCTTTT TCTGGATGCT GACTCTGGGC CTCATGCTCT TCTACCGCCT GGTTTTCATC
    CTGCATGACA CAAGCAAGTC CATTCAGAAG ACTATTGCAT TTTCTCTTGG CTATGGCTGC
    CCTCTTGTCA TCTCAGTCAT CACAGTGGGG GCCACCCAAC CCCAAGAAGT CTACACCAGG
    AAGAATGCCT GCTGGCTCAA CTGGGAGGAT ACCAAGGCCC TGCTGGCCTT TGTCATCCCA
    GCACTGATCA TCGTGGTGGT GAACATGACC ATCACGGTTG TGGTCATCTC CAAGATCCTG
    AGACCCTCCA TTGGAGACAA GCCAAGCAAG CAGGAGAAGA GTAGCTTGTT TCAGATCAGC
    AAGAGCATTG GGGTCCTAAC ACCACTCTTG GGGCTCACCT GGGGTTTTGG TCTTGCCACT
    GTGTTCCAAG GAAGCAATGC TGTGTTCCAT ATTATATTTA CACTCCTCAA TGCCTTTCAG
    GGATTATTCA TTTTGCTCTT TGGATGCCTC TGGGATCAGA AGGTACAGGA AGCCTTACTA
    AAGAAGTTTT CACTGTCAAG ATGGTCTTCT CAGCACTCAA AGTCAACATC CCTAGGTTCA
    TCTACACCAG TATTTTCTAT GAGTTCTCCA ATATCAAGAA GATTTAACAA TTTATTTGGA
    AAAACAGGAA CGTACAATGT TTCCACCCCA GAAACAACCA GCTCATCCCT GGAAAACACA
    TCCAGTGCTT ACTCCTTGCT GAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005627499.1
    CDS376..4542
    Translation

    Target ORF information:

    RefSeq Version XM_005627442.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005627442.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAATCCC CAAGGAGAAC CACATTGTGC TTCATGCTTA TTGTGATTTA TTTTTCCCAA 
    GCTACACTGA ACTTGAATTT AGAATCTATT GTTCATCCTT TGATTCTCCA TGAACATGAA
    CTAACAGAGA AGGAGCCACT AAGGGAAAAA CGAGCAGTTG CCTCCAGCAG CCCAGTACCT
    GCAGAGTACA CTGTTGATGT GGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAGCCTATC
    AAAGCTTATG TGAACAGCCT CAGTTTCCCA ATTCCAGGGA ACAGCACAGA CCTGGCCACC
    AACATTTTAA GCATTGAGGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CGGGCTATAG GTGGCCTCAG GAGAAGTGCC TCCACAATCC CACTTGTCAA
    GAGCATGACA GCTCCCTTGC AGGGCATCGT TGCAGTTGCC TGAAAGGACT ACCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGCAGAT GTTACTTTAA GAATGTCAGT CAGACTCAAT
    GTGGGCTTTC AAGAGGACCT CAAGAATTCT TCCTCTGCTC TCTATAGGTC CTACAAGACT
    GACCTGGAAA CAGCGTTCTG GAAAGGTTAC AGTACTTTAC CAGGCTTCAA ATTGGTGACT
    GTGACAGGGT TCAGGCCCGG AAGTGTGGTT GTGACATATG AGGTGAAGAC TACAACACCA
    TCACCTCAAT TGATGCATAA AGCAAATGAG CAAGTGATGC AGAACCTGAA TCAGACCTAC
    AGAATGGACT ACAGTTCCTT TCAAGCCGTT ACTAGCAATG AAACTAAGTT CACCATCATA
    CCAGAAATCA TTTTTGAAGG GGATACGGTA AACATGATGT GTGAAACCGA CGTTTTGTCC
    TCTAACGTGT CCTGGCACCA TGTTGAAAGG CACTCTGACA TCCAGAACAG CAGCAGATTC
    TCAGTTTACA CCACCGTGCT GAACAACATG ACCTCAGTCT CCCGCCTCAC GATTTACAAC
    ATCACCCAGC GGGATGCAGG TGAATATATT TGCAAGCTGA CATTAGATAT TTTTGAATAT
    GAGTCCAGAA AAAAAATAGA TGTCACGCCC ATCCGAATTC TGGCAACTGA AGAAATGAAG
    GTGACGTGCG ACAACAATCC TATATCTTTG AACTGCTGCA GTGAGATTAA TGCTAACTGG
    AGCACAATAG AATGGAAGCA GGAGGGGAAA ATAAACATTC CAGGCAACCC TGAAACTGAC
    CTAGAGTCCA GCTGCAGCAG ATACACCCTC AAGGCAGATG GAACCCAGTG TCCGAGTGGG
    TCGGCTGGAA CCACGGGCAT CTTTGTGTGT GAGTTCATCA GCACCTACGG AGCCAGAGGC
    AGTAAGAGCA TAGAAGTGAC GTTCATCTCT GTGGGCGATA ATTCCAGCCA CAAAGGGGAA
    GTGCACGAGA TCCTCAGGCA TGATACGGAA ATGAGTACAG TTCAGAGCAC CACCGGGAAC
    AGCCTAGAAA CAAGTAAAGC CCAGGAGCAG CAAGCCAACC TAACAATAAC CCCGGACCCA
    ATTTCTGTTT CTGAGGGACA AAACTTTTCC ATAAAGTGCA TCAGCGATGT GAGTAACTAT
    GATGAGGTAT ACTGGAACAC GTCTGCTGGA ATTAAAATAT ACCAAAAGTT TTATACCACG
    AGGAGGTTTT CTGATGGAGC AGAGTCAGTG CTGACAGTGA AGACTGTGAC CCGGGAGTGG
    AACGGAACCT ATCACTGCAT ATTTAGATAC AAGAATTCAT ACAGTGTCGT AATGAAAGAT
    GTGACCGTTT ACCCACTGCC TCTAGAGCCA AACATCATGG TTGATCCTCT GGAGGCTACA
    GTTCCCTGCA AGGGTTCCCA TCACTTCAGA TGCTGCATTG ACGAGGATGA GAACTACAGA
    GTGACTTTCC AAGTGGATTC CTTATCCTTT CCTGCAGAAA AAGAAGTTAA TGGCAAGCAA
    GTGTGCTACA AATACAAGTT CATGGCAAGG ACATCAGTTT CCTGGTGTCT AAAAAAGCTG
    GACATATTTT GTCACTTTAC CAATGCTGCT AATAATTCAG TCCGGAGCCC ATCTATGAAG
    CTTAATGTGG TTCCTGGAAA GAACATCATG TGCCGAGATC CCGTAATAGG TGTTGGGGAG
    CCTGGAAAAG TCATCCAGAA ACTCTGCCAT TTCTCAAAAG TACCAAACAT CTCTGGAGGT
    CCTACTGGAG GGATCATAAC TTACAAATGT GTAGGCTCCC AATGGGAGGT GAAGAAAAAT
    GACTGCATCT CTGCCCCAAT AAATAGTCTG CTCCAGCTGG CCAAGGCTTT GACCAAGAGC
    CCCACTCAGG ATAAGAAGCT CCCAACATAC CTAAAGGATC TTTCTATTAG CACAGGCAAG
    GTAAAACATG AAGTCAGCTC ATTTCCTGGG AGCCTGGGAG CCATCGTTAA CATCCTTGAT
    TTGCTTTCAA CAGTTACAAC CCAAGTGAAT TCAGAGATGA TGATGAACGT TCTCTCTACG
    GTTAACACTG TCCTCAGCAA GCCCATGTTG AGTACCTGGA AGGTGTCCCA ACAGCAACAG
    ACCAACCAGA GCTCACAGCT ACTGGATTCA GTAGAAAGAT TTTCCCGAGC ATTACAGACA
    GAAGATAGCA CCTTTTTATC CATCAACCAA ACTAACGTGC AGATGAGGAG CATGGTAATA
    AAAACTGGCC ATCCAAAAAC CTACAAACAG AGCTTTGTTT TCTCAGACTT GGACCTCTGG
    GGCAATGTGG CCATTGACAA GTGCGAGGTG GGAAGCCTGC AGCAAGATTC ATCTATTGTC
    ACCGTGGCTT TCCCAACTCT CCAAACCATC CTCACTCAGG ATGTTCAAGG AAAGAATTTT
    GCCAACAGCT TAGTGATGAC AACCACTGTT AGCCACAACA TAACCATGCC ATTTAGGATT
    TCAATGACTT TTAAGAACAT CAATCCTTCA GGTGGGGTAC CACAATGTGT CTACTGGAAT
    TTCAACCTCG CCAACCACAC AGGGGGTTGG GATAGTAGTG GGTGTTATGT GCACAATGTC
    GATGGGGACA GTGTCTCCTG CATCTGTGAC CACCTAACAT CATTCTCCAT CCTCATGTCC
    CCCGACTCTC CGTACCCTGG TTCCCTCCTG GGAATACTAC TGGATATCAT TTCCTACATT
    GGGTTATGCT TTTCCATCTT GAGCTTAGCA GCCTGTCTAG TGGTGGAAGC TGTGGTGTGG
    AAATCAGTGA CCAAGAATCG GACTTCCTAT ATGCGCCACA CCTGCATAGT GAACATTGCA
    GCCTCTCTTC TGGTTGCTGA CATCTGGTTC ATTGTAGCTG CTGCCATCCA CGACCATTTT
    TACCCACTCA ATGAGACAGC CTGTGTGGCC GCCACCTTTT TCATCCACTT CTTCTACCTC
    AGCGTCTTTT TCTGGATGCT GACTCTGGGC CTCATGCTCT TCTACCGCCT GGTTTTCATC
    CTGCATGACA CAAGCAAGTC CATTCAGAAG ACTATTGCAT TTTCTCTTGG CTATGGCTGC
    CCTCTTGTCA TCTCAGTCAT CACAGTGGGG GCCACCCAAC CCCAAGAAGT CTACACCAGG
    AAGAATGCCT GCTGGCTCAA CTGGGAGGAT ACCAAGGCCC TGCTGGCCTT TGTCATCCCA
    GCACTGATCA TCGTGGTGGT GAACATGACC ATCACGGTTG TGGTCATCTC CAAGATCCTG
    AGACCCTCCA TTGGAGACAA GCCAAGCAAG CAGGAGAAGA GTAGCTTGTT TCAGATCAGC
    AAGAGCATTG GGGTCCTAAC ACCACTCTTG GGGCTCACCT GGGGTTTTGG TCTTGCCACT
    GTGTTCCAAG GAAGCAATGC TGTGTTCCAT ATTATATTTA CACTCCTCAA TGCCTTTCAG
    GGATTATTCA TTTTGCTCTT TGGATGCCTC TGGGATCAGA AGGTACAGGA AGCCTTACTA
    AAGAAGTTTT CACTGTCAAG ATGGTCTTCT CAGCACTCAA AGTCAACATC CCTAGGTTCA
    TCTACACCAG TATTTTCTAT GAGTTCTCCA ATATCAAGAA GATTTAACAA TTTATTTGGA
    AAAACAGGAA CGTACAATGT TTCCACCCCA GAAACAACCA GCTCATCCCT GGAAAACACA
    TCCAGTGCTT ACTCCTTGCT GAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe15371
    Clone ID Related Accession (Same CDS sequence) XM_005627442.2 , XM_005627441.3 , XM_022426127.1 , XM_005627442.3 , XM_005627441.2
    Accession Version XM_005627441.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4167bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product adhesion G protein-coupled receptor F5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005627441.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAATCCC CAAGGAGAAC CACATTGTGC TTCATGCTTA TTGTGATTTA TTTTTCCCAA 
    GCTACACTGA ACTTGAATTT AGAATCTATT GTTCATCCTT TGATTCTCCA TGAACATGAA
    CTAACAGAGA AGGAGCCACT AAGGGAAAAA CGAGCAGTTG CCTCCAGCAG CCCAGTACCT
    GCAGAGTACA CTGTTGATGT GGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAGCCTATC
    AAAGCTTATG TGAACAGCCT CAGTTTCCCA ATTCCAGGGA ACAGCACAGA CCTGGCCACC
    AACATTTTAA GCATTGAGGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CGGGCTATAG GTGGCCTCAG GAGAAGTGCC TCCACAATCC CACTTGTCAA
    GAGCATGACA GCTCCCTTGC AGGGCATCGT TGCAGTTGCC TGAAAGGACT ACCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGCAGAT GTTACTTTAA GAATGTCAGT CAGACTCAAT
    GTGGGCTTTC AAGAGGACCT CAAGAATTCT TCCTCTGCTC TCTATAGGTC CTACAAGACT
    GACCTGGAAA CAGCGTTCTG GAAAGGTTAC AGTACTTTAC CAGGCTTCAA ATTGGTGACT
    GTGACAGGGT TCAGGCCCGG AAGTGTGGTT GTGACATATG AGGTGAAGAC TACAACACCA
    TCACCTCAAT TGATGCATAA AGCAAATGAG CAAGTGATGC AGAACCTGAA TCAGACCTAC
    AGAATGGACT ACAGTTCCTT TCAAGCCGTT ACTAGCAATG AAACTAAGTT CACCATCATA
    CCAGAAATCA TTTTTGAAGG GGATACGGTA AACATGATGT GTGAAACCGA CGTTTTGTCC
    TCTAACGTGT CCTGGCACCA TGTTGAAAGG CACTCTGACA TCCAGAACAG CAGCAGATTC
    TCAGTTTACA CCACCGTGCT GAACAACATG ACCTCAGTCT CCCGCCTCAC GATTTACAAC
    ATCACCCAGC GGGATGCAGG TGAATATATT TGCAAGCTGA CATTAGATAT TTTTGAATAT
    GAGTCCAGAA AAAAAATAGA TGTCACGCCC ATCCGAATTC TGGCAACTGA AGAAATGAAG
    GTGACGTGCG ACAACAATCC TATATCTTTG AACTGCTGCA GTGAGATTAA TGCTAACTGG
    AGCACAATAG AATGGAAGCA GGAGGGGAAA ATAAACATTC CAGGCAACCC TGAAACTGAC
    CTAGAGTCCA GCTGCAGCAG ATACACCCTC AAGGCAGATG GAACCCAGTG TCCGAGTGGG
    TCGGCTGGAA CCACGGGCAT CTTTGTGTGT GAGTTCATCA GCACCTACGG AGCCAGAGGC
    AGTAAGAGCA TAGAAGTGAC GTTCATCTCT GTGGGCGATA ATTCCAGCCA CAAAGGGGAA
    GTGCACGAGA TCCTCAGGCA TGATACGGAA ATGAGTACAG TTCAGAGCAC CACCGGGAAC
    AGCCTAGAAA CAAGTAAAGC CCAGGAGCAG CAAGCCAACC TAACAATAAC CCCGGACCCA
    ATTTCTGTTT CTGAGGGACA AAACTTTTCC ATAAAGTGCA TCAGCGATGT GAGTAACTAT
    GATGAGGTAT ACTGGAACAC GTCTGCTGGA ATTAAAATAT ACCAAAAGTT TTATACCACG
    AGGAGGTTTT CTGATGGAGC AGAGTCAGTG CTGACAGTGA AGACTGTGAC CCGGGAGTGG
    AACGGAACCT ATCACTGCAT ATTTAGATAC AAGAATTCAT ACAGTGTCGT AATGAAAGAT
    GTGACCGTTT ACCCACTGCC TCTAGAGCCA AACATCATGG TTGATCCTCT GGAGGCTACA
    GTTCCCTGCA AGGGTTCCCA TCACTTCAGA TGCTGCATTG ACGAGGATGA GAACTACAGA
    GTGACTTTCC AAGTGGATTC CTTATCCTTT CCTGCAGAAA AAGAAGTTAA TGGCAAGCAA
    GTGTGCTACA AATACAAGTT CATGGCAAGG ACATCAGTTT CCTGGTGTCT AAAAAAGCTG
    GACATATTTT GTCACTTTAC CAATGCTGCT AATAATTCAG TCCGGAGCCC ATCTATGAAG
    CTTAATGTGG TTCCTGGAAA GAACATCATG TGCCGAGATC CCGTAATAGG TGTTGGGGAG
    CCTGGAAAAG TCATCCAGAA ACTCTGCCAT TTCTCAAAAG TACCAAACAT CTCTGGAGGT
    CCTACTGGAG GGATCATAAC TTACAAATGT GTAGGCTCCC AATGGGAGGT GAAGAAAAAT
    GACTGCATCT CTGCCCCAAT AAATAGTCTG CTCCAGCTGG CCAAGGCTTT GACCAAGAGC
    CCCACTCAGG ATAAGAAGCT CCCAACATAC CTAAAGGATC TTTCTATTAG CACAGGCAAG
    GTAAAACATG AAGTCAGCTC ATTTCCTGGG AGCCTGGGAG CCATCGTTAA CATCCTTGAT
    TTGCTTTCAA CAGTTACAAC CCAAGTGAAT TCAGAGATGA TGATGAACGT TCTCTCTACG
    GTTAACACTG TCCTCAGCAA GCCCATGTTG AGTACCTGGA AGGTGTCCCA ACAGCAACAG
    ACCAACCAGA GCTCACAGCT ACTGGATTCA GTAGAAAGAT TTTCCCGAGC ATTACAGACA
    GAAGATAGCA CCTTTTTATC CATCAACCAA ACTAACGTGC AGATGAGGAG CATGGTAATA
    AAAACTGGCC ATCCAAAAAC CTACAAACAG AGCTTTGTTT TCTCAGACTT GGACCTCTGG
    GGCAATGTGG CCATTGACAA GTGCGAGGTG GGAAGCCTGC AGCAAGATTC ATCTATTGTC
    ACCGTGGCTT TCCCAACTCT CCAAACCATC CTCACTCAGG ATGTTCAAGG AAAGAATTTT
    GCCAACAGCT TAGTGATGAC AACCACTGTT AGCCACAACA TAACCATGCC ATTTAGGATT
    TCAATGACTT TTAAGAACAT CAATCCTTCA GGTGGGGTAC CACAATGTGT CTACTGGAAT
    TTCAACCTCG CCAACCACAC AGGGGGTTGG GATAGTAGTG GGTGTTATGT GCACAATGTC
    GATGGGGACA GTGTCTCCTG CATCTGTGAC CACCTAACAT CATTCTCCAT CCTCATGTCC
    CCCGACTCTC CGTACCCTGG TTCCCTCCTG GGAATACTAC TGGATATCAT TTCCTACATT
    GGGTTATGCT TTTCCATCTT GAGCTTAGCA GCCTGTCTAG TGGTGGAAGC TGTGGTGTGG
    AAATCAGTGA CCAAGAATCG GACTTCCTAT ATGCGCCACA CCTGCATAGT GAACATTGCA
    GCCTCTCTTC TGGTTGCTGA CATCTGGTTC ATTGTAGCTG CTGCCATCCA CGACCATTTT
    TACCCACTCA ATGAGACAGC CTGTGTGGCC GCCACCTTTT TCATCCACTT CTTCTACCTC
    AGCGTCTTTT TCTGGATGCT GACTCTGGGC CTCATGCTCT TCTACCGCCT GGTTTTCATC
    CTGCATGACA CAAGCAAGTC CATTCAGAAG ACTATTGCAT TTTCTCTTGG CTATGGCTGC
    CCTCTTGTCA TCTCAGTCAT CACAGTGGGG GCCACCCAAC CCCAAGAAGT CTACACCAGG
    AAGAATGCCT GCTGGCTCAA CTGGGAGGAT ACCAAGGCCC TGCTGGCCTT TGTCATCCCA
    GCACTGATCA TCGTGGTGGT GAACATGACC ATCACGGTTG TGGTCATCTC CAAGATCCTG
    AGACCCTCCA TTGGAGACAA GCCAAGCAAG CAGGAGAAGA GTAGCTTGTT TCAGATCAGC
    AAGAGCATTG GGGTCCTAAC ACCACTCTTG GGGCTCACCT GGGGTTTTGG TCTTGCCACT
    GTGTTCCAAG GAAGCAATGC TGTGTTCCAT ATTATATTTA CACTCCTCAA TGCCTTTCAG
    GGATTATTCA TTTTGCTCTT TGGATGCCTC TGGGATCAGA AGGTACAGGA AGCCTTACTA
    AAGAAGTTTT CACTGTCAAG ATGGTCTTCT CAGCACTCAA AGTCAACATC CCTAGGTTCA
    TCTACACCAG TATTTTCTAT GAGTTCTCCA ATATCAAGAA GATTTAACAA TTTATTTGGA
    AAAACAGGAA CGTACAATGT TTCCACCCCA GAAACAACCA GCTCATCCCT GGAAAACACA
    TCCAGTGCTT ACTCCTTGCT GAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005627498.1
    CDS416..4582
    Translation

    Target ORF information:

    RefSeq Version XM_005627441.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005627441.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAATCCC CAAGGAGAAC CACATTGTGC TTCATGCTTA TTGTGATTTA TTTTTCCCAA 
    GCTACACTGA ACTTGAATTT AGAATCTATT GTTCATCCTT TGATTCTCCA TGAACATGAA
    CTAACAGAGA AGGAGCCACT AAGGGAAAAA CGAGCAGTTG CCTCCAGCAG CCCAGTACCT
    GCAGAGTACA CTGTTGATGT GGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAGCCTATC
    AAAGCTTATG TGAACAGCCT CAGTTTCCCA ATTCCAGGGA ACAGCACAGA CCTGGCCACC
    AACATTTTAA GCATTGAGGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CGGGCTATAG GTGGCCTCAG GAGAAGTGCC TCCACAATCC CACTTGTCAA
    GAGCATGACA GCTCCCTTGC AGGGCATCGT TGCAGTTGCC TGAAAGGACT ACCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGCAGAT GTTACTTTAA GAATGTCAGT CAGACTCAAT
    GTGGGCTTTC AAGAGGACCT CAAGAATTCT TCCTCTGCTC TCTATAGGTC CTACAAGACT
    GACCTGGAAA CAGCGTTCTG GAAAGGTTAC AGTACTTTAC CAGGCTTCAA ATTGGTGACT
    GTGACAGGGT TCAGGCCCGG AAGTGTGGTT GTGACATATG AGGTGAAGAC TACAACACCA
    TCACCTCAAT TGATGCATAA AGCAAATGAG CAAGTGATGC AGAACCTGAA TCAGACCTAC
    AGAATGGACT ACAGTTCCTT TCAAGCCGTT ACTAGCAATG AAACTAAGTT CACCATCATA
    CCAGAAATCA TTTTTGAAGG GGATACGGTA AACATGATGT GTGAAACCGA CGTTTTGTCC
    TCTAACGTGT CCTGGCACCA TGTTGAAAGG CACTCTGACA TCCAGAACAG CAGCAGATTC
    TCAGTTTACA CCACCGTGCT GAACAACATG ACCTCAGTCT CCCGCCTCAC GATTTACAAC
    ATCACCCAGC GGGATGCAGG TGAATATATT TGCAAGCTGA CATTAGATAT TTTTGAATAT
    GAGTCCAGAA AAAAAATAGA TGTCACGCCC ATCCGAATTC TGGCAACTGA AGAAATGAAG
    GTGACGTGCG ACAACAATCC TATATCTTTG AACTGCTGCA GTGAGATTAA TGCTAACTGG
    AGCACAATAG AATGGAAGCA GGAGGGGAAA ATAAACATTC CAGGCAACCC TGAAACTGAC
    CTAGAGTCCA GCTGCAGCAG ATACACCCTC AAGGCAGATG GAACCCAGTG TCCGAGTGGG
    TCGGCTGGAA CCACGGGCAT CTTTGTGTGT GAGTTCATCA GCACCTACGG AGCCAGAGGC
    AGTAAGAGCA TAGAAGTGAC GTTCATCTCT GTGGGCGATA ATTCCAGCCA CAAAGGGGAA
    GTGCACGAGA TCCTCAGGCA TGATACGGAA ATGAGTACAG TTCAGAGCAC CACCGGGAAC
    AGCCTAGAAA CAAGTAAAGC CCAGGAGCAG CAAGCCAACC TAACAATAAC CCCGGACCCA
    ATTTCTGTTT CTGAGGGACA AAACTTTTCC ATAAAGTGCA TCAGCGATGT GAGTAACTAT
    GATGAGGTAT ACTGGAACAC GTCTGCTGGA ATTAAAATAT ACCAAAAGTT TTATACCACG
    AGGAGGTTTT CTGATGGAGC AGAGTCAGTG CTGACAGTGA AGACTGTGAC CCGGGAGTGG
    AACGGAACCT ATCACTGCAT ATTTAGATAC AAGAATTCAT ACAGTGTCGT AATGAAAGAT
    GTGACCGTTT ACCCACTGCC TCTAGAGCCA AACATCATGG TTGATCCTCT GGAGGCTACA
    GTTCCCTGCA AGGGTTCCCA TCACTTCAGA TGCTGCATTG ACGAGGATGA GAACTACAGA
    GTGACTTTCC AAGTGGATTC CTTATCCTTT CCTGCAGAAA AAGAAGTTAA TGGCAAGCAA
    GTGTGCTACA AATACAAGTT CATGGCAAGG ACATCAGTTT CCTGGTGTCT AAAAAAGCTG
    GACATATTTT GTCACTTTAC CAATGCTGCT AATAATTCAG TCCGGAGCCC ATCTATGAAG
    CTTAATGTGG TTCCTGGAAA GAACATCATG TGCCGAGATC CCGTAATAGG TGTTGGGGAG
    CCTGGAAAAG TCATCCAGAA ACTCTGCCAT TTCTCAAAAG TACCAAACAT CTCTGGAGGT
    CCTACTGGAG GGATCATAAC TTACAAATGT GTAGGCTCCC AATGGGAGGT GAAGAAAAAT
    GACTGCATCT CTGCCCCAAT AAATAGTCTG CTCCAGCTGG CCAAGGCTTT GACCAAGAGC
    CCCACTCAGG ATAAGAAGCT CCCAACATAC CTAAAGGATC TTTCTATTAG CACAGGCAAG
    GTAAAACATG AAGTCAGCTC ATTTCCTGGG AGCCTGGGAG CCATCGTTAA CATCCTTGAT
    TTGCTTTCAA CAGTTACAAC CCAAGTGAAT TCAGAGATGA TGATGAACGT TCTCTCTACG
    GTTAACACTG TCCTCAGCAA GCCCATGTTG AGTACCTGGA AGGTGTCCCA ACAGCAACAG
    ACCAACCAGA GCTCACAGCT ACTGGATTCA GTAGAAAGAT TTTCCCGAGC ATTACAGACA
    GAAGATAGCA CCTTTTTATC CATCAACCAA ACTAACGTGC AGATGAGGAG CATGGTAATA
    AAAACTGGCC ATCCAAAAAC CTACAAACAG AGCTTTGTTT TCTCAGACTT GGACCTCTGG
    GGCAATGTGG CCATTGACAA GTGCGAGGTG GGAAGCCTGC AGCAAGATTC ATCTATTGTC
    ACCGTGGCTT TCCCAACTCT CCAAACCATC CTCACTCAGG ATGTTCAAGG AAAGAATTTT
    GCCAACAGCT TAGTGATGAC AACCACTGTT AGCCACAACA TAACCATGCC ATTTAGGATT
    TCAATGACTT TTAAGAACAT CAATCCTTCA GGTGGGGTAC CACAATGTGT CTACTGGAAT
    TTCAACCTCG CCAACCACAC AGGGGGTTGG GATAGTAGTG GGTGTTATGT GCACAATGTC
    GATGGGGACA GTGTCTCCTG CATCTGTGAC CACCTAACAT CATTCTCCAT CCTCATGTCC
    CCCGACTCTC CGTACCCTGG TTCCCTCCTG GGAATACTAC TGGATATCAT TTCCTACATT
    GGGTTATGCT TTTCCATCTT GAGCTTAGCA GCCTGTCTAG TGGTGGAAGC TGTGGTGTGG
    AAATCAGTGA CCAAGAATCG GACTTCCTAT ATGCGCCACA CCTGCATAGT GAACATTGCA
    GCCTCTCTTC TGGTTGCTGA CATCTGGTTC ATTGTAGCTG CTGCCATCCA CGACCATTTT
    TACCCACTCA ATGAGACAGC CTGTGTGGCC GCCACCTTTT TCATCCACTT CTTCTACCTC
    AGCGTCTTTT TCTGGATGCT GACTCTGGGC CTCATGCTCT TCTACCGCCT GGTTTTCATC
    CTGCATGACA CAAGCAAGTC CATTCAGAAG ACTATTGCAT TTTCTCTTGG CTATGGCTGC
    CCTCTTGTCA TCTCAGTCAT CACAGTGGGG GCCACCCAAC CCCAAGAAGT CTACACCAGG
    AAGAATGCCT GCTGGCTCAA CTGGGAGGAT ACCAAGGCCC TGCTGGCCTT TGTCATCCCA
    GCACTGATCA TCGTGGTGGT GAACATGACC ATCACGGTTG TGGTCATCTC CAAGATCCTG
    AGACCCTCCA TTGGAGACAA GCCAAGCAAG CAGGAGAAGA GTAGCTTGTT TCAGATCAGC
    AAGAGCATTG GGGTCCTAAC ACCACTCTTG GGGCTCACCT GGGGTTTTGG TCTTGCCACT
    GTGTTCCAAG GAAGCAATGC TGTGTTCCAT ATTATATTTA CACTCCTCAA TGCCTTTCAG
    GGATTATTCA TTTTGCTCTT TGGATGCCTC TGGGATCAGA AGGTACAGGA AGCCTTACTA
    AAGAAGTTTT CACTGTCAAG ATGGTCTTCT CAGCACTCAA AGTCAACATC CCTAGGTTCA
    TCTACACCAG TATTTTCTAT GAGTTCTCCA ATATCAAGAA GATTTAACAA TTTATTTGGA
    AAAACAGGAA CGTACAATGT TTCCACCCCA GAAACAACCA GCTCATCCCT GGAAAACACA
    TCCAGTGCTT ACTCCTTGCT GAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe15371
    Clone ID Related Accession (Same CDS sequence) XM_005627442.2 , XM_005627441.3 , XM_022426127.1 , XM_005627442.3 , XM_005627441.2
    Accession Version XM_022426127.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4167bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product adhesion G protein-coupled receptor F5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAATCCC CAAGGAGAAC CACATTGTGC TTCATGCTTA TTGTGATTTA TTTTTCCCAA 
    GCTACACTGA ACTTGAATTT AGAATCTATT GTTCATCCTT TGATTCTCCA TGAACATGAA
    CTAACAGAGA AGGAGCCACT AAGGGAAAAA CGAGCAGTTG CCTCCAGCAG CCCAGTACCT
    GCAGAGTACA CTGTTGATGT GGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAGCCTATC
    AAAGCTTATG TGAACAGCCT CAGTTTCCCA ATTCCAGGGA ACAGCACAGA CCTGGCCACC
    AACATTTTAA GCATTGAGGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CGGGCTATAG GTGGCCTCAG GAGAAGTGCC TCCACAATCC CACTTGTCAA
    GAGCATGACA GCTCCCTTGC AGGGCATCGT TGCAGTTGCC TGAAAGGACT ACCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGCAGAT GTTACTTTAA GAATGTCAGT CAGACTCAAT
    GTGGGCTTTC AAGAGGACCT CAAGAATTCT TCCTCTGCTC TCTATAGGTC CTACAAGACT
    GACCTGGAAA CAGCGTTCTG GAAAGGTTAC AGTACTTTAC CAGGCTTCAA ATTGGTGACT
    GTGACAGGGT TCAGGCCCGG AAGTGTGGTT GTGACATATG AGGTGAAGAC TACAACACCA
    TCACCTCAAT TGATGCATAA AGCAAATGAG CAAGTGATGC AGAACCTGAA TCAGACCTAC
    AGAATGGACT ACAGTTCCTT TCAAGCCGTT ACTAGCAATG AAACTAAGTT CACCATCATA
    CCAGAAATCA TTTTTGAAGG GGATACGGTA AACATGATGT GTGAAACCGA CGTTTTGTCC
    TCTAACGTGT CCTGGCACCA TGTTGAAAGG CACTCTGACA TCCAGAACAG CAGCAGATTC
    TCAGTTTACA CCACCGTGCT GAACAACATG ACCTCAGTCT CCCGCCTCAC GATTTACAAC
    ATCACCCAGC GGGATGCAGG TGAATATATT TGCAAGCTGA CATTAGATAT TTTTGAATAT
    GAGTCCAGAA AAAAAATAGA TGTCACGCCC ATCCGAATTC TGGCAACTGA AGAAATGAAG
    GTGACGTGCG ACAACAATCC TATATCTTTG AACTGCTGCA GTGAGATTAA TGCTAACTGG
    AGCACAATAG AATGGAAGCA GGAGGGGAAA ATAAACATTC CAGGCAACCC TGAAACTGAC
    CTAGAGTCCA GCTGCAGCAG ATACACCCTC AAGGCAGATG GAACCCAGTG TCCGAGTGGG
    TCGGCTGGAA CCACGGGCAT CTTTGTGTGT GAGTTCATCA GCACCTACGG AGCCAGAGGC
    AGTAAGAGCA TAGAAGTGAC GTTCATCTCT GTGGGCGATA ATTCCAGCCA CAAAGGGGAA
    GTGCACGAGA TCCTCAGGCA TGATACGGAA ATGAGTACAG TTCAGAGCAC CACCGGGAAC
    AGCCTAGAAA CAAGTAAAGC CCAGGAGCAG CAAGCCAACC TAACAATAAC CCCGGACCCA
    ATTTCTGTTT CTGAGGGACA AAACTTTTCC ATAAAGTGCA TCAGCGATGT GAGTAACTAT
    GATGAGGTAT ACTGGAACAC GTCTGCTGGA ATTAAAATAT ACCAAAAGTT TTATACCACG
    AGGAGGTTTT CTGATGGAGC AGAGTCAGTG CTGACAGTGA AGACTGTGAC CCGGGAGTGG
    AACGGAACCT ATCACTGCAT ATTTAGATAC AAGAATTCAT ACAGTGTCGT AATGAAAGAT
    GTGACCGTTT ACCCACTGCC TCTAGAGCCA AACATCATGG TTGATCCTCT GGAGGCTACA
    GTTCCCTGCA AGGGTTCCCA TCACTTCAGA TGCTGCATTG ACGAGGATGA GAACTACAGA
    GTGACTTTCC AAGTGGATTC CTTATCCTTT CCTGCAGAAA AAGAAGTTAA TGGCAAGCAA
    GTGTGCTACA AATACAAGTT CATGGCAAGG ACATCAGTTT CCTGGTGTCT AAAAAAGCTG
    GACATATTTT GTCACTTTAC CAATGCTGCT AATAATTCAG TCCGGAGCCC ATCTATGAAG
    CTTAATGTGG TTCCTGGAAA GAACATCATG TGCCGAGATC CCGTAATAGG TGTTGGGGAG
    CCTGGAAAAG TCATCCAGAA ACTCTGCCAT TTCTCAAAAG TACCAAACAT CTCTGGAGGT
    CCTACTGGAG GGATCATAAC TTACAAATGT GTAGGCTCCC AATGGGAGGT GAAGAAAAAT
    GACTGCATCT CTGCCCCAAT AAATAGTCTG CTCCAGCTGG CCAAGGCTTT GACCAAGAGC
    CCCACTCAGG ATAAGAAGCT CCCAACATAC CTAAAGGATC TTTCTATTAG CACAGGCAAG
    GTAAAACATG AAGTCAGCTC ATTTCCTGGG AGCCTGGGAG CCATCGTTAA CATCCTTGAT
    TTGCTTTCAA CAGTTACAAC CCAAGTGAAT TCAGAGATGA TGATGAACGT TCTCTCTACG
    GTTAACACTG TCCTCAGCAA GCCCATGTTG AGTACCTGGA AGGTGTCCCA ACAGCAACAG
    ACCAACCAGA GCTCACAGCT ACTGGATTCA GTAGAAAGAT TTTCCCGAGC ATTACAGACA
    GAAGATAGCA CCTTTTTATC CATCAACCAA ACTAACGTGC AGATGAGGAG CATGGTAATA
    AAAACTGGCC ATCCAAAAAC CTACAAACAG AGCTTTGTTT TCTCAGACTT GGACCTCTGG
    GGCAATGTGG CCATTGACAA GTGCGAGGTG GGAAGCCTGC AGCAAGATTC ATCTATTGTC
    ACCGTGGCTT TCCCAACTCT CCAAACCATC CTCACTCAGG ATGTTCAAGG AAAGAATTTT
    GCCAACAGCT TAGTGATGAC AACCACTGTT AGCCACAACA TAACCATGCC ATTTAGGATT
    TCAATGACTT TTAAGAACAT CAATCCTTCA GGTGGGGTAC CACAATGTGT CTACTGGAAT
    TTCAACCTCG CCAACCACAC AGGGGGTTGG GATAGTAGTG GGTGTTATGT GCACAATGTC
    GATGGGGACA GTGTCTCCTG CATCTGTGAC CACCTAACAT CATTCTCCAT CCTCATGTCC
    CCCGACTCTC CGTACCCTGG TTCCCTCCTG GGAATACTAC TGGATATCAT TTCCTACATT
    GGGTTATGCT TTTCCATCTT GAGCTTAGCA GCCTGTCTAG TGGTGGAAGC TGTGGTGTGG
    AAATCAGTGA CCAAGAATCG GACTTCCTAT ATGCGCCACA CCTGCATAGT GAACATTGCA
    GCCTCTCTTC TGGTTGCTGA CATCTGGTTC ATTGTAGCTG CTGCCATCCA CGACCATTTT
    TACCCACTCA ATGAGACAGC CTGTGTGGCC GCCACCTTTT TCATCCACTT CTTCTACCTC
    AGCGTCTTTT TCTGGATGCT GACTCTGGGC CTCATGCTCT TCTACCGCCT GGTTTTCATC
    CTGCATGACA CAAGCAAGTC CATTCAGAAG ACTATTGCAT TTTCTCTTGG CTATGGCTGC
    CCTCTTGTCA TCTCAGTCAT CACAGTGGGG GCCACCCAAC CCCAAGAAGT CTACACCAGG
    AAGAATGCCT GCTGGCTCAA CTGGGAGGAT ACCAAGGCCC TGCTGGCCTT TGTCATCCCA
    GCACTGATCA TCGTGGTGGT GAACATGACC ATCACGGTTG TGGTCATCTC CAAGATCCTG
    AGACCCTCCA TTGGAGACAA GCCAAGCAAG CAGGAGAAGA GTAGCTTGTT TCAGATCAGC
    AAGAGCATTG GGGTCCTAAC ACCACTCTTG GGGCTCACCT GGGGTTTTGG TCTTGCCACT
    GTGTTCCAAG GAAGCAATGC TGTGTTCCAT ATTATATTTA CACTCCTCAA TGCCTTTCAG
    GGATTATTCA TTTTGCTCTT TGGATGCCTC TGGGATCAGA AGGTACAGGA AGCCTTACTA
    AAGAAGTTTT CACTGTCAAG ATGGTCTTCT CAGCACTCAA AGTCAACATC CCTAGGTTCA
    TCTACACCAG TATTTTCTAT GAGTTCTCCA ATATCAAGAA GATTTAACAA TTTATTTGGA
    AAAACAGGAA CGTACAATGT TTCCACCCCA GAAACAACCA GCTCATCCCT GGAAAACACA
    TCCAGTGCTT ACTCCTTGCT GAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022281835.1
    CDS509..4675
    Translation

    Target ORF information:

    RefSeq Version XM_022426127.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426127.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAATCCC CAAGGAGAAC CACATTGTGC TTCATGCTTA TTGTGATTTA TTTTTCCCAA 
    GCTACACTGA ACTTGAATTT AGAATCTATT GTTCATCCTT TGATTCTCCA TGAACATGAA
    CTAACAGAGA AGGAGCCACT AAGGGAAAAA CGAGCAGTTG CCTCCAGCAG CCCAGTACCT
    GCAGAGTACA CTGTTGATGT GGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAGCCTATC
    AAAGCTTATG TGAACAGCCT CAGTTTCCCA ATTCCAGGGA ACAGCACAGA CCTGGCCACC
    AACATTTTAA GCATTGAGGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CGGGCTATAG GTGGCCTCAG GAGAAGTGCC TCCACAATCC CACTTGTCAA
    GAGCATGACA GCTCCCTTGC AGGGCATCGT TGCAGTTGCC TGAAAGGACT ACCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGCAGAT GTTACTTTAA GAATGTCAGT CAGACTCAAT
    GTGGGCTTTC AAGAGGACCT CAAGAATTCT TCCTCTGCTC TCTATAGGTC CTACAAGACT
    GACCTGGAAA CAGCGTTCTG GAAAGGTTAC AGTACTTTAC CAGGCTTCAA ATTGGTGACT
    GTGACAGGGT TCAGGCCCGG AAGTGTGGTT GTGACATATG AGGTGAAGAC TACAACACCA
    TCACCTCAAT TGATGCATAA AGCAAATGAG CAAGTGATGC AGAACCTGAA TCAGACCTAC
    AGAATGGACT ACAGTTCCTT TCAAGCCGTT ACTAGCAATG AAACTAAGTT CACCATCATA
    CCAGAAATCA TTTTTGAAGG GGATACGGTA AACATGATGT GTGAAACCGA CGTTTTGTCC
    TCTAACGTGT CCTGGCACCA TGTTGAAAGG CACTCTGACA TCCAGAACAG CAGCAGATTC
    TCAGTTTACA CCACCGTGCT GAACAACATG ACCTCAGTCT CCCGCCTCAC GATTTACAAC
    ATCACCCAGC GGGATGCAGG TGAATATATT TGCAAGCTGA CATTAGATAT TTTTGAATAT
    GAGTCCAGAA AAAAAATAGA TGTCACGCCC ATCCGAATTC TGGCAACTGA AGAAATGAAG
    GTGACGTGCG ACAACAATCC TATATCTTTG AACTGCTGCA GTGAGATTAA TGCTAACTGG
    AGCACAATAG AATGGAAGCA GGAGGGGAAA ATAAACATTC CAGGCAACCC TGAAACTGAC
    CTAGAGTCCA GCTGCAGCAG ATACACCCTC AAGGCAGATG GAACCCAGTG TCCGAGTGGG
    TCGGCTGGAA CCACGGGCAT CTTTGTGTGT GAGTTCATCA GCACCTACGG AGCCAGAGGC
    AGTAAGAGCA TAGAAGTGAC GTTCATCTCT GTGGGCGATA ATTCCAGCCA CAAAGGGGAA
    GTGCACGAGA TCCTCAGGCA TGATACGGAA ATGAGTACAG TTCAGAGCAC CACCGGGAAC
    AGCCTAGAAA CAAGTAAAGC CCAGGAGCAG CAAGCCAACC TAACAATAAC CCCGGACCCA
    ATTTCTGTTT CTGAGGGACA AAACTTTTCC ATAAAGTGCA TCAGCGATGT GAGTAACTAT
    GATGAGGTAT ACTGGAACAC GTCTGCTGGA ATTAAAATAT ACCAAAAGTT TTATACCACG
    AGGAGGTTTT CTGATGGAGC AGAGTCAGTG CTGACAGTGA AGACTGTGAC CCGGGAGTGG
    AACGGAACCT ATCACTGCAT ATTTAGATAC AAGAATTCAT ACAGTGTCGT AATGAAAGAT
    GTGACCGTTT ACCCACTGCC TCTAGAGCCA AACATCATGG TTGATCCTCT GGAGGCTACA
    GTTCCCTGCA AGGGTTCCCA TCACTTCAGA TGCTGCATTG ACGAGGATGA GAACTACAGA
    GTGACTTTCC AAGTGGATTC CTTATCCTTT CCTGCAGAAA AAGAAGTTAA TGGCAAGCAA
    GTGTGCTACA AATACAAGTT CATGGCAAGG ACATCAGTTT CCTGGTGTCT AAAAAAGCTG
    GACATATTTT GTCACTTTAC CAATGCTGCT AATAATTCAG TCCGGAGCCC ATCTATGAAG
    CTTAATGTGG TTCCTGGAAA GAACATCATG TGCCGAGATC CCGTAATAGG TGTTGGGGAG
    CCTGGAAAAG TCATCCAGAA ACTCTGCCAT TTCTCAAAAG TACCAAACAT CTCTGGAGGT
    CCTACTGGAG GGATCATAAC TTACAAATGT GTAGGCTCCC AATGGGAGGT GAAGAAAAAT
    GACTGCATCT CTGCCCCAAT AAATAGTCTG CTCCAGCTGG CCAAGGCTTT GACCAAGAGC
    CCCACTCAGG ATAAGAAGCT CCCAACATAC CTAAAGGATC TTTCTATTAG CACAGGCAAG
    GTAAAACATG AAGTCAGCTC ATTTCCTGGG AGCCTGGGAG CCATCGTTAA CATCCTTGAT
    TTGCTTTCAA CAGTTACAAC CCAAGTGAAT TCAGAGATGA TGATGAACGT TCTCTCTACG
    GTTAACACTG TCCTCAGCAA GCCCATGTTG AGTACCTGGA AGGTGTCCCA ACAGCAACAG
    ACCAACCAGA GCTCACAGCT ACTGGATTCA GTAGAAAGAT TTTCCCGAGC ATTACAGACA
    GAAGATAGCA CCTTTTTATC CATCAACCAA ACTAACGTGC AGATGAGGAG CATGGTAATA
    AAAACTGGCC ATCCAAAAAC CTACAAACAG AGCTTTGTTT TCTCAGACTT GGACCTCTGG
    GGCAATGTGG CCATTGACAA GTGCGAGGTG GGAAGCCTGC AGCAAGATTC ATCTATTGTC
    ACCGTGGCTT TCCCAACTCT CCAAACCATC CTCACTCAGG ATGTTCAAGG AAAGAATTTT
    GCCAACAGCT TAGTGATGAC AACCACTGTT AGCCACAACA TAACCATGCC ATTTAGGATT
    TCAATGACTT TTAAGAACAT CAATCCTTCA GGTGGGGTAC CACAATGTGT CTACTGGAAT
    TTCAACCTCG CCAACCACAC AGGGGGTTGG GATAGTAGTG GGTGTTATGT GCACAATGTC
    GATGGGGACA GTGTCTCCTG CATCTGTGAC CACCTAACAT CATTCTCCAT CCTCATGTCC
    CCCGACTCTC CGTACCCTGG TTCCCTCCTG GGAATACTAC TGGATATCAT TTCCTACATT
    GGGTTATGCT TTTCCATCTT GAGCTTAGCA GCCTGTCTAG TGGTGGAAGC TGTGGTGTGG
    AAATCAGTGA CCAAGAATCG GACTTCCTAT ATGCGCCACA CCTGCATAGT GAACATTGCA
    GCCTCTCTTC TGGTTGCTGA CATCTGGTTC ATTGTAGCTG CTGCCATCCA CGACCATTTT
    TACCCACTCA ATGAGACAGC CTGTGTGGCC GCCACCTTTT TCATCCACTT CTTCTACCTC
    AGCGTCTTTT TCTGGATGCT GACTCTGGGC CTCATGCTCT TCTACCGCCT GGTTTTCATC
    CTGCATGACA CAAGCAAGTC CATTCAGAAG ACTATTGCAT TTTCTCTTGG CTATGGCTGC
    CCTCTTGTCA TCTCAGTCAT CACAGTGGGG GCCACCCAAC CCCAAGAAGT CTACACCAGG
    AAGAATGCCT GCTGGCTCAA CTGGGAGGAT ACCAAGGCCC TGCTGGCCTT TGTCATCCCA
    GCACTGATCA TCGTGGTGGT GAACATGACC ATCACGGTTG TGGTCATCTC CAAGATCCTG
    AGACCCTCCA TTGGAGACAA GCCAAGCAAG CAGGAGAAGA GTAGCTTGTT TCAGATCAGC
    AAGAGCATTG GGGTCCTAAC ACCACTCTTG GGGCTCACCT GGGGTTTTGG TCTTGCCACT
    GTGTTCCAAG GAAGCAATGC TGTGTTCCAT ATTATATTTA CACTCCTCAA TGCCTTTCAG
    GGATTATTCA TTTTGCTCTT TGGATGCCTC TGGGATCAGA AGGTACAGGA AGCCTTACTA
    AAGAAGTTTT CACTGTCAAG ATGGTCTTCT CAGCACTCAA AGTCAACATC CCTAGGTTCA
    TCTACACCAG TATTTTCTAT GAGTTCTCCA ATATCAAGAA GATTTAACAA TTTATTTGGA
    AAAACAGGAA CGTACAATGT TTCCACCCCA GAAACAACCA GCTCATCCCT GGAAAACACA
    TCCAGTGCTT ACTCCTTGCT GAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe15371
    Clone ID Related Accession (Same CDS sequence) XM_005627442.2 , XM_005627441.3 , XM_022426127.1 , XM_005627442.3 , XM_005627441.2
    Accession Version XM_005627442.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4167bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product adhesion G protein-coupled receptor F5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005627442.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAATCCC CAAGGAGAAC CACATTGTGC TTCATGCTTA TTGTGATTTA TTTTTCCCAA 
    GCTACACTGA ACTTGAATTT AGAATCTATT GTTCATCCTT TGATTCTCCA TGAACATGAA
    CTAACAGAGA AGGAGCCACT AAGGGAAAAA CGAGCAGTTG CCTCCAGCAG CCCAGTACCT
    GCAGAGTACA CTGTTGATGT GGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAGCCTATC
    AAAGCTTATG TGAACAGCCT CAGTTTCCCA ATTCCAGGGA ACAGCACAGA CCTGGCCACC
    AACATTTTAA GCATTGAGGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CGGGCTATAG GTGGCCTCAG GAGAAGTGCC TCCACAATCC CACTTGTCAA
    GAGCATGACA GCTCCCTTGC AGGGCATCGT TGCAGTTGCC TGAAAGGACT ACCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGCAGAT GTTACTTTAA GAATGTCAGT CAGACTCAAT
    GTGGGCTTTC AAGAGGACCT CAAGAATTCT TCCTCTGCTC TCTATAGGTC CTACAAGACT
    GACCTGGAAA CAGCGTTCTG GAAAGGTTAC AGTACTTTAC CAGGCTTCAA ATTGGTGACT
    GTGACAGGGT TCAGGCCCGG AAGTGTGGTT GTGACATATG AGGTGAAGAC TACAACACCA
    TCACCTCAAT TGATGCATAA AGCAAATGAG CAAGTGATGC AGAACCTGAA TCAGACCTAC
    AGAATGGACT ACAGTTCCTT TCAAGCCGTT ACTAGCAATG AAACTAAGTT CACCATCATA
    CCAGAAATCA TTTTTGAAGG GGATACGGTA AACATGATGT GTGAAACCGA CGTTTTGTCC
    TCTAACGTGT CCTGGCACCA TGTTGAAAGG CACTCTGACA TCCAGAACAG CAGCAGATTC
    TCAGTTTACA CCACCGTGCT GAACAACATG ACCTCAGTCT CCCGCCTCAC GATTTACAAC
    ATCACCCAGC GGGATGCAGG TGAATATATT TGCAAGCTGA CATTAGATAT TTTTGAATAT
    GAGTCCAGAA AAAAAATAGA TGTCACGCCC ATCCGAATTC TGGCAACTGA AGAAATGAAG
    GTGACGTGCG ACAACAATCC TATATCTTTG AACTGCTGCA GTGAGATTAA TGCTAACTGG
    AGCACAATAG AATGGAAGCA GGAGGGGAAA ATAAACATTC CAGGCAACCC TGAAACTGAC
    CTAGAGTCCA GCTGCAGCAG ATACACCCTC AAGGCAGATG GAACCCAGTG TCCGAGTGGG
    TCGGCTGGAA CCACGGGCAT CTTTGTGTGT GAGTTCATCA GCACCTACGG AGCCAGAGGC
    AGTAAGAGCA TAGAAGTGAC GTTCATCTCT GTGGGCGATA ATTCCAGCCA CAAAGGGGAA
    GTGCACGAGA TCCTCAGGCA TGATACGGAA ATGAGTACAG TTCAGAGCAC CACCGGGAAC
    AGCCTAGAAA CAAGTAAAGC CCAGGAGCAG CAAGCCAACC TAACAATAAC CCCGGACCCA
    ATTTCTGTTT CTGAGGGACA AAACTTTTCC ATAAAGTGCA TCAGCGATGT GAGTAACTAT
    GATGAGGTAT ACTGGAACAC GTCTGCTGGA ATTAAAATAT ACCAAAAGTT TTATACCACG
    AGGAGGTTTT CTGATGGAGC AGAGTCAGTG CTGACAGTGA AGACTGTGAC CCGGGAGTGG
    AACGGAACCT ATCACTGCAT ATTTAGATAC AAGAATTCAT ACAGTGTCGT AATGAAAGAT
    GTGACCGTTT ACCCACTGCC TCTAGAGCCA AACATCATGG TTGATCCTCT GGAGGCTACA
    GTTCCCTGCA AGGGTTCCCA TCACTTCAGA TGCTGCATTG ACGAGGATGA GAACTACAGA
    GTGACTTTCC AAGTGGATTC CTTATCCTTT CCTGCAGAAA AAGAAGTTAA TGGCAAGCAA
    GTGTGCTACA AATACAAGTT CATGGCAAGG ACATCAGTTT CCTGGTGTCT AAAAAAGCTG
    GACATATTTT GTCACTTTAC CAATGCTGCT AATAATTCAG TCCGGAGCCC ATCTATGAAG
    CTTAATGTGG TTCCTGGAAA GAACATCATG TGCCGAGATC CCGTAATAGG TGTTGGGGAG
    CCTGGAAAAG TCATCCAGAA ACTCTGCCAT TTCTCAAAAG TACCAAACAT CTCTGGAGGT
    CCTACTGGAG GGATCATAAC TTACAAATGT GTAGGCTCCC AATGGGAGGT GAAGAAAAAT
    GACTGCATCT CTGCCCCAAT AAATAGTCTG CTCCAGCTGG CCAAGGCTTT GACCAAGAGC
    CCCACTCAGG ATAAGAAGCT CCCAACATAC CTAAAGGATC TTTCTATTAG CACAGGCAAG
    GTAAAACATG AAGTCAGCTC ATTTCCTGGG AGCCTGGGAG CCATCGTTAA CATCCTTGAT
    TTGCTTTCAA CAGTTACAAC CCAAGTGAAT TCAGAGATGA TGATGAACGT TCTCTCTACG
    GTTAACACTG TCCTCAGCAA GCCCATGTTG AGTACCTGGA AGGTGTCCCA ACAGCAACAG
    ACCAACCAGA GCTCACAGCT ACTGGATTCA GTAGAAAGAT TTTCCCGAGC ATTACAGACA
    GAAGATAGCA CCTTTTTATC CATCAACCAA ACTAACGTGC AGATGAGGAG CATGGTAATA
    AAAACTGGCC ATCCAAAAAC CTACAAACAG AGCTTTGTTT TCTCAGACTT GGACCTCTGG
    GGCAATGTGG CCATTGACAA GTGCGAGGTG GGAAGCCTGC AGCAAGATTC ATCTATTGTC
    ACCGTGGCTT TCCCAACTCT CCAAACCATC CTCACTCAGG ATGTTCAAGG AAAGAATTTT
    GCCAACAGCT TAGTGATGAC AACCACTGTT AGCCACAACA TAACCATGCC ATTTAGGATT
    TCAATGACTT TTAAGAACAT CAATCCTTCA GGTGGGGTAC CACAATGTGT CTACTGGAAT
    TTCAACCTCG CCAACCACAC AGGGGGTTGG GATAGTAGTG GGTGTTATGT GCACAATGTC
    GATGGGGACA GTGTCTCCTG CATCTGTGAC CACCTAACAT CATTCTCCAT CCTCATGTCC
    CCCGACTCTC CGTACCCTGG TTCCCTCCTG GGAATACTAC TGGATATCAT TTCCTACATT
    GGGTTATGCT TTTCCATCTT GAGCTTAGCA GCCTGTCTAG TGGTGGAAGC TGTGGTGTGG
    AAATCAGTGA CCAAGAATCG GACTTCCTAT ATGCGCCACA CCTGCATAGT GAACATTGCA
    GCCTCTCTTC TGGTTGCTGA CATCTGGTTC ATTGTAGCTG CTGCCATCCA CGACCATTTT
    TACCCACTCA ATGAGACAGC CTGTGTGGCC GCCACCTTTT TCATCCACTT CTTCTACCTC
    AGCGTCTTTT TCTGGATGCT GACTCTGGGC CTCATGCTCT TCTACCGCCT GGTTTTCATC
    CTGCATGACA CAAGCAAGTC CATTCAGAAG ACTATTGCAT TTTCTCTTGG CTATGGCTGC
    CCTCTTGTCA TCTCAGTCAT CACAGTGGGG GCCACCCAAC CCCAAGAAGT CTACACCAGG
    AAGAATGCCT GCTGGCTCAA CTGGGAGGAT ACCAAGGCCC TGCTGGCCTT TGTCATCCCA
    GCACTGATCA TCGTGGTGGT GAACATGACC ATCACGGTTG TGGTCATCTC CAAGATCCTG
    AGACCCTCCA TTGGAGACAA GCCAAGCAAG CAGGAGAAGA GTAGCTTGTT TCAGATCAGC
    AAGAGCATTG GGGTCCTAAC ACCACTCTTG GGGCTCACCT GGGGTTTTGG TCTTGCCACT
    GTGTTCCAAG GAAGCAATGC TGTGTTCCAT ATTATATTTA CACTCCTCAA TGCCTTTCAG
    GGATTATTCA TTTTGCTCTT TGGATGCCTC TGGGATCAGA AGGTACAGGA AGCCTTACTA
    AAGAAGTTTT CACTGTCAAG ATGGTCTTCT CAGCACTCAA AGTCAACATC CCTAGGTTCA
    TCTACACCAG TATTTTCTAT GAGTTCTCCA ATATCAAGAA GATTTAACAA TTTATTTGGA
    AAAACAGGAA CGTACAATGT TTCCACCCCA GAAACAACCA GCTCATCCCT GGAAAACACA
    TCCAGTGCTT ACTCCTTGCT GAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005627499.1
    CDS364..4530
    Translation

    Target ORF information:

    RefSeq Version XM_005627442.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005627442.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAATCCC CAAGGAGAAC CACATTGTGC TTCATGCTTA TTGTGATTTA TTTTTCCCAA 
    GCTACACTGA ACTTGAATTT AGAATCTATT GTTCATCCTT TGATTCTCCA TGAACATGAA
    CTAACAGAGA AGGAGCCACT AAGGGAAAAA CGAGCAGTTG CCTCCAGCAG CCCAGTACCT
    GCAGAGTACA CTGTTGATGT GGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAGCCTATC
    AAAGCTTATG TGAACAGCCT CAGTTTCCCA ATTCCAGGGA ACAGCACAGA CCTGGCCACC
    AACATTTTAA GCATTGAGGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CGGGCTATAG GTGGCCTCAG GAGAAGTGCC TCCACAATCC CACTTGTCAA
    GAGCATGACA GCTCCCTTGC AGGGCATCGT TGCAGTTGCC TGAAAGGACT ACCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGCAGAT GTTACTTTAA GAATGTCAGT CAGACTCAAT
    GTGGGCTTTC AAGAGGACCT CAAGAATTCT TCCTCTGCTC TCTATAGGTC CTACAAGACT
    GACCTGGAAA CAGCGTTCTG GAAAGGTTAC AGTACTTTAC CAGGCTTCAA ATTGGTGACT
    GTGACAGGGT TCAGGCCCGG AAGTGTGGTT GTGACATATG AGGTGAAGAC TACAACACCA
    TCACCTCAAT TGATGCATAA AGCAAATGAG CAAGTGATGC AGAACCTGAA TCAGACCTAC
    AGAATGGACT ACAGTTCCTT TCAAGCCGTT ACTAGCAATG AAACTAAGTT CACCATCATA
    CCAGAAATCA TTTTTGAAGG GGATACGGTA AACATGATGT GTGAAACCGA CGTTTTGTCC
    TCTAACGTGT CCTGGCACCA TGTTGAAAGG CACTCTGACA TCCAGAACAG CAGCAGATTC
    TCAGTTTACA CCACCGTGCT GAACAACATG ACCTCAGTCT CCCGCCTCAC GATTTACAAC
    ATCACCCAGC GGGATGCAGG TGAATATATT TGCAAGCTGA CATTAGATAT TTTTGAATAT
    GAGTCCAGAA AAAAAATAGA TGTCACGCCC ATCCGAATTC TGGCAACTGA AGAAATGAAG
    GTGACGTGCG ACAACAATCC TATATCTTTG AACTGCTGCA GTGAGATTAA TGCTAACTGG
    AGCACAATAG AATGGAAGCA GGAGGGGAAA ATAAACATTC CAGGCAACCC TGAAACTGAC
    CTAGAGTCCA GCTGCAGCAG ATACACCCTC AAGGCAGATG GAACCCAGTG TCCGAGTGGG
    TCGGCTGGAA CCACGGGCAT CTTTGTGTGT GAGTTCATCA GCACCTACGG AGCCAGAGGC
    AGTAAGAGCA TAGAAGTGAC GTTCATCTCT GTGGGCGATA ATTCCAGCCA CAAAGGGGAA
    GTGCACGAGA TCCTCAGGCA TGATACGGAA ATGAGTACAG TTCAGAGCAC CACCGGGAAC
    AGCCTAGAAA CAAGTAAAGC CCAGGAGCAG CAAGCCAACC TAACAATAAC CCCGGACCCA
    ATTTCTGTTT CTGAGGGACA AAACTTTTCC ATAAAGTGCA TCAGCGATGT GAGTAACTAT
    GATGAGGTAT ACTGGAACAC GTCTGCTGGA ATTAAAATAT ACCAAAAGTT TTATACCACG
    AGGAGGTTTT CTGATGGAGC AGAGTCAGTG CTGACAGTGA AGACTGTGAC CCGGGAGTGG
    AACGGAACCT ATCACTGCAT ATTTAGATAC AAGAATTCAT ACAGTGTCGT AATGAAAGAT
    GTGACCGTTT ACCCACTGCC TCTAGAGCCA AACATCATGG TTGATCCTCT GGAGGCTACA
    GTTCCCTGCA AGGGTTCCCA TCACTTCAGA TGCTGCATTG ACGAGGATGA GAACTACAGA
    GTGACTTTCC AAGTGGATTC CTTATCCTTT CCTGCAGAAA AAGAAGTTAA TGGCAAGCAA
    GTGTGCTACA AATACAAGTT CATGGCAAGG ACATCAGTTT CCTGGTGTCT AAAAAAGCTG
    GACATATTTT GTCACTTTAC CAATGCTGCT AATAATTCAG TCCGGAGCCC ATCTATGAAG
    CTTAATGTGG TTCCTGGAAA GAACATCATG TGCCGAGATC CCGTAATAGG TGTTGGGGAG
    CCTGGAAAAG TCATCCAGAA ACTCTGCCAT TTCTCAAAAG TACCAAACAT CTCTGGAGGT
    CCTACTGGAG GGATCATAAC TTACAAATGT GTAGGCTCCC AATGGGAGGT GAAGAAAAAT
    GACTGCATCT CTGCCCCAAT AAATAGTCTG CTCCAGCTGG CCAAGGCTTT GACCAAGAGC
    CCCACTCAGG ATAAGAAGCT CCCAACATAC CTAAAGGATC TTTCTATTAG CACAGGCAAG
    GTAAAACATG AAGTCAGCTC ATTTCCTGGG AGCCTGGGAG CCATCGTTAA CATCCTTGAT
    TTGCTTTCAA CAGTTACAAC CCAAGTGAAT TCAGAGATGA TGATGAACGT TCTCTCTACG
    GTTAACACTG TCCTCAGCAA GCCCATGTTG AGTACCTGGA AGGTGTCCCA ACAGCAACAG
    ACCAACCAGA GCTCACAGCT ACTGGATTCA GTAGAAAGAT TTTCCCGAGC ATTACAGACA
    GAAGATAGCA CCTTTTTATC CATCAACCAA ACTAACGTGC AGATGAGGAG CATGGTAATA
    AAAACTGGCC ATCCAAAAAC CTACAAACAG AGCTTTGTTT TCTCAGACTT GGACCTCTGG
    GGCAATGTGG CCATTGACAA GTGCGAGGTG GGAAGCCTGC AGCAAGATTC ATCTATTGTC
    ACCGTGGCTT TCCCAACTCT CCAAACCATC CTCACTCAGG ATGTTCAAGG AAAGAATTTT
    GCCAACAGCT TAGTGATGAC AACCACTGTT AGCCACAACA TAACCATGCC ATTTAGGATT
    TCAATGACTT TTAAGAACAT CAATCCTTCA GGTGGGGTAC CACAATGTGT CTACTGGAAT
    TTCAACCTCG CCAACCACAC AGGGGGTTGG GATAGTAGTG GGTGTTATGT GCACAATGTC
    GATGGGGACA GTGTCTCCTG CATCTGTGAC CACCTAACAT CATTCTCCAT CCTCATGTCC
    CCCGACTCTC CGTACCCTGG TTCCCTCCTG GGAATACTAC TGGATATCAT TTCCTACATT
    GGGTTATGCT TTTCCATCTT GAGCTTAGCA GCCTGTCTAG TGGTGGAAGC TGTGGTGTGG
    AAATCAGTGA CCAAGAATCG GACTTCCTAT ATGCGCCACA CCTGCATAGT GAACATTGCA
    GCCTCTCTTC TGGTTGCTGA CATCTGGTTC ATTGTAGCTG CTGCCATCCA CGACCATTTT
    TACCCACTCA ATGAGACAGC CTGTGTGGCC GCCACCTTTT TCATCCACTT CTTCTACCTC
    AGCGTCTTTT TCTGGATGCT GACTCTGGGC CTCATGCTCT TCTACCGCCT GGTTTTCATC
    CTGCATGACA CAAGCAAGTC CATTCAGAAG ACTATTGCAT TTTCTCTTGG CTATGGCTGC
    CCTCTTGTCA TCTCAGTCAT CACAGTGGGG GCCACCCAAC CCCAAGAAGT CTACACCAGG
    AAGAATGCCT GCTGGCTCAA CTGGGAGGAT ACCAAGGCCC TGCTGGCCTT TGTCATCCCA
    GCACTGATCA TCGTGGTGGT GAACATGACC ATCACGGTTG TGGTCATCTC CAAGATCCTG
    AGACCCTCCA TTGGAGACAA GCCAAGCAAG CAGGAGAAGA GTAGCTTGTT TCAGATCAGC
    AAGAGCATTG GGGTCCTAAC ACCACTCTTG GGGCTCACCT GGGGTTTTGG TCTTGCCACT
    GTGTTCCAAG GAAGCAATGC TGTGTTCCAT ATTATATTTA CACTCCTCAA TGCCTTTCAG
    GGATTATTCA TTTTGCTCTT TGGATGCCTC TGGGATCAGA AGGTACAGGA AGCCTTACTA
    AAGAAGTTTT CACTGTCAAG ATGGTCTTCT CAGCACTCAA AGTCAACATC CCTAGGTTCA
    TCTACACCAG TATTTTCTAT GAGTTCTCCA ATATCAAGAA GATTTAACAA TTTATTTGGA
    AAAACAGGAA CGTACAATGT TTCCACCCCA GAAACAACCA GCTCATCCCT GGAAAACACA
    TCCAGTGCTT ACTCCTTGCT GAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe15371
    Clone ID Related Accession (Same CDS sequence) XM_005627442.2 , XM_005627441.3 , XM_022426127.1 , XM_005627442.3 , XM_005627441.2
    Accession Version XM_005627441.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4167bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product adhesion G protein-coupled receptor F5 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005627441.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAATCCC CAAGGAGAAC CACATTGTGC TTCATGCTTA TTGTGATTTA TTTTTCCCAA 
    GCTACACTGA ACTTGAATTT AGAATCTATT GTTCATCCTT TGATTCTCCA TGAACATGAA
    CTAACAGAGA AGGAGCCACT AAGGGAAAAA CGAGCAGTTG CCTCCAGCAG CCCAGTACCT
    GCAGAGTACA CTGTTGATGT GGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAGCCTATC
    AAAGCTTATG TGAACAGCCT CAGTTTCCCA ATTCCAGGGA ACAGCACAGA CCTGGCCACC
    AACATTTTAA GCATTGAGGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CGGGCTATAG GTGGCCTCAG GAGAAGTGCC TCCACAATCC CACTTGTCAA
    GAGCATGACA GCTCCCTTGC AGGGCATCGT TGCAGTTGCC TGAAAGGACT ACCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGCAGAT GTTACTTTAA GAATGTCAGT CAGACTCAAT
    GTGGGCTTTC AAGAGGACCT CAAGAATTCT TCCTCTGCTC TCTATAGGTC CTACAAGACT
    GACCTGGAAA CAGCGTTCTG GAAAGGTTAC AGTACTTTAC CAGGCTTCAA ATTGGTGACT
    GTGACAGGGT TCAGGCCCGG AAGTGTGGTT GTGACATATG AGGTGAAGAC TACAACACCA
    TCACCTCAAT TGATGCATAA AGCAAATGAG CAAGTGATGC AGAACCTGAA TCAGACCTAC
    AGAATGGACT ACAGTTCCTT TCAAGCCGTT ACTAGCAATG AAACTAAGTT CACCATCATA
    CCAGAAATCA TTTTTGAAGG GGATACGGTA AACATGATGT GTGAAACCGA CGTTTTGTCC
    TCTAACGTGT CCTGGCACCA TGTTGAAAGG CACTCTGACA TCCAGAACAG CAGCAGATTC
    TCAGTTTACA CCACCGTGCT GAACAACATG ACCTCAGTCT CCCGCCTCAC GATTTACAAC
    ATCACCCAGC GGGATGCAGG TGAATATATT TGCAAGCTGA CATTAGATAT TTTTGAATAT
    GAGTCCAGAA AAAAAATAGA TGTCACGCCC ATCCGAATTC TGGCAACTGA AGAAATGAAG
    GTGACGTGCG ACAACAATCC TATATCTTTG AACTGCTGCA GTGAGATTAA TGCTAACTGG
    AGCACAATAG AATGGAAGCA GGAGGGGAAA ATAAACATTC CAGGCAACCC TGAAACTGAC
    CTAGAGTCCA GCTGCAGCAG ATACACCCTC AAGGCAGATG GAACCCAGTG TCCGAGTGGG
    TCGGCTGGAA CCACGGGCAT CTTTGTGTGT GAGTTCATCA GCACCTACGG AGCCAGAGGC
    AGTAAGAGCA TAGAAGTGAC GTTCATCTCT GTGGGCGATA ATTCCAGCCA CAAAGGGGAA
    GTGCACGAGA TCCTCAGGCA TGATACGGAA ATGAGTACAG TTCAGAGCAC CACCGGGAAC
    AGCCTAGAAA CAAGTAAAGC CCAGGAGCAG CAAGCCAACC TAACAATAAC CCCGGACCCA
    ATTTCTGTTT CTGAGGGACA AAACTTTTCC ATAAAGTGCA TCAGCGATGT GAGTAACTAT
    GATGAGGTAT ACTGGAACAC GTCTGCTGGA ATTAAAATAT ACCAAAAGTT TTATACCACG
    AGGAGGTTTT CTGATGGAGC AGAGTCAGTG CTGACAGTGA AGACTGTGAC CCGGGAGTGG
    AACGGAACCT ATCACTGCAT ATTTAGATAC AAGAATTCAT ACAGTGTCGT AATGAAAGAT
    GTGACCGTTT ACCCACTGCC TCTAGAGCCA AACATCATGG TTGATCCTCT GGAGGCTACA
    GTTCCCTGCA AGGGTTCCCA TCACTTCAGA TGCTGCATTG ACGAGGATGA GAACTACAGA
    GTGACTTTCC AAGTGGATTC CTTATCCTTT CCTGCAGAAA AAGAAGTTAA TGGCAAGCAA
    GTGTGCTACA AATACAAGTT CATGGCAAGG ACATCAGTTT CCTGGTGTCT AAAAAAGCTG
    GACATATTTT GTCACTTTAC CAATGCTGCT AATAATTCAG TCCGGAGCCC ATCTATGAAG
    CTTAATGTGG TTCCTGGAAA GAACATCATG TGCCGAGATC CCGTAATAGG TGTTGGGGAG
    CCTGGAAAAG TCATCCAGAA ACTCTGCCAT TTCTCAAAAG TACCAAACAT CTCTGGAGGT
    CCTACTGGAG GGATCATAAC TTACAAATGT GTAGGCTCCC AATGGGAGGT GAAGAAAAAT
    GACTGCATCT CTGCCCCAAT AAATAGTCTG CTCCAGCTGG CCAAGGCTTT GACCAAGAGC
    CCCACTCAGG ATAAGAAGCT CCCAACATAC CTAAAGGATC TTTCTATTAG CACAGGCAAG
    GTAAAACATG AAGTCAGCTC ATTTCCTGGG AGCCTGGGAG CCATCGTTAA CATCCTTGAT
    TTGCTTTCAA CAGTTACAAC CCAAGTGAAT TCAGAGATGA TGATGAACGT TCTCTCTACG
    GTTAACACTG TCCTCAGCAA GCCCATGTTG AGTACCTGGA AGGTGTCCCA ACAGCAACAG
    ACCAACCAGA GCTCACAGCT ACTGGATTCA GTAGAAAGAT TTTCCCGAGC ATTACAGACA
    GAAGATAGCA CCTTTTTATC CATCAACCAA ACTAACGTGC AGATGAGGAG CATGGTAATA
    AAAACTGGCC ATCCAAAAAC CTACAAACAG AGCTTTGTTT TCTCAGACTT GGACCTCTGG
    GGCAATGTGG CCATTGACAA GTGCGAGGTG GGAAGCCTGC AGCAAGATTC ATCTATTGTC
    ACCGTGGCTT TCCCAACTCT CCAAACCATC CTCACTCAGG ATGTTCAAGG AAAGAATTTT
    GCCAACAGCT TAGTGATGAC AACCACTGTT AGCCACAACA TAACCATGCC ATTTAGGATT
    TCAATGACTT TTAAGAACAT CAATCCTTCA GGTGGGGTAC CACAATGTGT CTACTGGAAT
    TTCAACCTCG CCAACCACAC AGGGGGTTGG GATAGTAGTG GGTGTTATGT GCACAATGTC
    GATGGGGACA GTGTCTCCTG CATCTGTGAC CACCTAACAT CATTCTCCAT CCTCATGTCC
    CCCGACTCTC CGTACCCTGG TTCCCTCCTG GGAATACTAC TGGATATCAT TTCCTACATT
    GGGTTATGCT TTTCCATCTT GAGCTTAGCA GCCTGTCTAG TGGTGGAAGC TGTGGTGTGG
    AAATCAGTGA CCAAGAATCG GACTTCCTAT ATGCGCCACA CCTGCATAGT GAACATTGCA
    GCCTCTCTTC TGGTTGCTGA CATCTGGTTC ATTGTAGCTG CTGCCATCCA CGACCATTTT
    TACCCACTCA ATGAGACAGC CTGTGTGGCC GCCACCTTTT TCATCCACTT CTTCTACCTC
    AGCGTCTTTT TCTGGATGCT GACTCTGGGC CTCATGCTCT TCTACCGCCT GGTTTTCATC
    CTGCATGACA CAAGCAAGTC CATTCAGAAG ACTATTGCAT TTTCTCTTGG CTATGGCTGC
    CCTCTTGTCA TCTCAGTCAT CACAGTGGGG GCCACCCAAC CCCAAGAAGT CTACACCAGG
    AAGAATGCCT GCTGGCTCAA CTGGGAGGAT ACCAAGGCCC TGCTGGCCTT TGTCATCCCA
    GCACTGATCA TCGTGGTGGT GAACATGACC ATCACGGTTG TGGTCATCTC CAAGATCCTG
    AGACCCTCCA TTGGAGACAA GCCAAGCAAG CAGGAGAAGA GTAGCTTGTT TCAGATCAGC
    AAGAGCATTG GGGTCCTAAC ACCACTCTTG GGGCTCACCT GGGGTTTTGG TCTTGCCACT
    GTGTTCCAAG GAAGCAATGC TGTGTTCCAT ATTATATTTA CACTCCTCAA TGCCTTTCAG
    GGATTATTCA TTTTGCTCTT TGGATGCCTC TGGGATCAGA AGGTACAGGA AGCCTTACTA
    AAGAAGTTTT CACTGTCAAG ATGGTCTTCT CAGCACTCAA AGTCAACATC CCTAGGTTCA
    TCTACACCAG TATTTTCTAT GAGTTCTCCA ATATCAAGAA GATTTAACAA TTTATTTGGA
    AAAACAGGAA CGTACAATGT TTCCACCCCA GAAACAACCA GCTCATCCCT GGAAAACACA
    TCCAGTGCTT ACTCCTTGCT GAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005627498.1
    CDS398..4564
    Translation

    Target ORF information:

    RefSeq Version XM_005627441.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005627441.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAATCCC CAAGGAGAAC CACATTGTGC TTCATGCTTA TTGTGATTTA TTTTTCCCAA 
    GCTACACTGA ACTTGAATTT AGAATCTATT GTTCATCCTT TGATTCTCCA TGAACATGAA
    CTAACAGAGA AGGAGCCACT AAGGGAAAAA CGAGCAGTTG CCTCCAGCAG CCCAGTACCT
    GCAGAGTACA CTGTTGATGT GGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAGCCTATC
    AAAGCTTATG TGAACAGCCT CAGTTTCCCA ATTCCAGGGA ACAGCACAGA CCTGGCCACC
    AACATTTTAA GCATTGAGGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CGGGCTATAG GTGGCCTCAG GAGAAGTGCC TCCACAATCC CACTTGTCAA
    GAGCATGACA GCTCCCTTGC AGGGCATCGT TGCAGTTGCC TGAAAGGACT ACCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGCAGAT GTTACTTTAA GAATGTCAGT CAGACTCAAT
    GTGGGCTTTC AAGAGGACCT CAAGAATTCT TCCTCTGCTC TCTATAGGTC CTACAAGACT
    GACCTGGAAA CAGCGTTCTG GAAAGGTTAC AGTACTTTAC CAGGCTTCAA ATTGGTGACT
    GTGACAGGGT TCAGGCCCGG AAGTGTGGTT GTGACATATG AGGTGAAGAC TACAACACCA
    TCACCTCAAT TGATGCATAA AGCAAATGAG CAAGTGATGC AGAACCTGAA TCAGACCTAC
    AGAATGGACT ACAGTTCCTT TCAAGCCGTT ACTAGCAATG AAACTAAGTT CACCATCATA
    CCAGAAATCA TTTTTGAAGG GGATACGGTA AACATGATGT GTGAAACCGA CGTTTTGTCC
    TCTAACGTGT CCTGGCACCA TGTTGAAAGG CACTCTGACA TCCAGAACAG CAGCAGATTC
    TCAGTTTACA CCACCGTGCT GAACAACATG ACCTCAGTCT CCCGCCTCAC GATTTACAAC
    ATCACCCAGC GGGATGCAGG TGAATATATT TGCAAGCTGA CATTAGATAT TTTTGAATAT
    GAGTCCAGAA AAAAAATAGA TGTCACGCCC ATCCGAATTC TGGCAACTGA AGAAATGAAG
    GTGACGTGCG ACAACAATCC TATATCTTTG AACTGCTGCA GTGAGATTAA TGCTAACTGG
    AGCACAATAG AATGGAAGCA GGAGGGGAAA ATAAACATTC CAGGCAACCC TGAAACTGAC
    CTAGAGTCCA GCTGCAGCAG ATACACCCTC AAGGCAGATG GAACCCAGTG TCCGAGTGGG
    TCGGCTGGAA CCACGGGCAT CTTTGTGTGT GAGTTCATCA GCACCTACGG AGCCAGAGGC
    AGTAAGAGCA TAGAAGTGAC GTTCATCTCT GTGGGCGATA ATTCCAGCCA CAAAGGGGAA
    GTGCACGAGA TCCTCAGGCA TGATACGGAA ATGAGTACAG TTCAGAGCAC CACCGGGAAC
    AGCCTAGAAA CAAGTAAAGC CCAGGAGCAG CAAGCCAACC TAACAATAAC CCCGGACCCA
    ATTTCTGTTT CTGAGGGACA AAACTTTTCC ATAAAGTGCA TCAGCGATGT GAGTAACTAT
    GATGAGGTAT ACTGGAACAC GTCTGCTGGA ATTAAAATAT ACCAAAAGTT TTATACCACG
    AGGAGGTTTT CTGATGGAGC AGAGTCAGTG CTGACAGTGA AGACTGTGAC CCGGGAGTGG
    AACGGAACCT ATCACTGCAT ATTTAGATAC AAGAATTCAT ACAGTGTCGT AATGAAAGAT
    GTGACCGTTT ACCCACTGCC TCTAGAGCCA AACATCATGG TTGATCCTCT GGAGGCTACA
    GTTCCCTGCA AGGGTTCCCA TCACTTCAGA TGCTGCATTG ACGAGGATGA GAACTACAGA
    GTGACTTTCC AAGTGGATTC CTTATCCTTT CCTGCAGAAA AAGAAGTTAA TGGCAAGCAA
    GTGTGCTACA AATACAAGTT CATGGCAAGG ACATCAGTTT CCTGGTGTCT AAAAAAGCTG
    GACATATTTT GTCACTTTAC CAATGCTGCT AATAATTCAG TCCGGAGCCC ATCTATGAAG
    CTTAATGTGG TTCCTGGAAA GAACATCATG TGCCGAGATC CCGTAATAGG TGTTGGGGAG
    CCTGGAAAAG TCATCCAGAA ACTCTGCCAT TTCTCAAAAG TACCAAACAT CTCTGGAGGT
    CCTACTGGAG GGATCATAAC TTACAAATGT GTAGGCTCCC AATGGGAGGT GAAGAAAAAT
    GACTGCATCT CTGCCCCAAT AAATAGTCTG CTCCAGCTGG CCAAGGCTTT GACCAAGAGC
    CCCACTCAGG ATAAGAAGCT CCCAACATAC CTAAAGGATC TTTCTATTAG CACAGGCAAG
    GTAAAACATG AAGTCAGCTC ATTTCCTGGG AGCCTGGGAG CCATCGTTAA CATCCTTGAT
    TTGCTTTCAA CAGTTACAAC CCAAGTGAAT TCAGAGATGA TGATGAACGT TCTCTCTACG
    GTTAACACTG TCCTCAGCAA GCCCATGTTG AGTACCTGGA AGGTGTCCCA ACAGCAACAG
    ACCAACCAGA GCTCACAGCT ACTGGATTCA GTAGAAAGAT TTTCCCGAGC ATTACAGACA
    GAAGATAGCA CCTTTTTATC CATCAACCAA ACTAACGTGC AGATGAGGAG CATGGTAATA
    AAAACTGGCC ATCCAAAAAC CTACAAACAG AGCTTTGTTT TCTCAGACTT GGACCTCTGG
    GGCAATGTGG CCATTGACAA GTGCGAGGTG GGAAGCCTGC AGCAAGATTC ATCTATTGTC
    ACCGTGGCTT TCCCAACTCT CCAAACCATC CTCACTCAGG ATGTTCAAGG AAAGAATTTT
    GCCAACAGCT TAGTGATGAC AACCACTGTT AGCCACAACA TAACCATGCC ATTTAGGATT
    TCAATGACTT TTAAGAACAT CAATCCTTCA GGTGGGGTAC CACAATGTGT CTACTGGAAT
    TTCAACCTCG CCAACCACAC AGGGGGTTGG GATAGTAGTG GGTGTTATGT GCACAATGTC
    GATGGGGACA GTGTCTCCTG CATCTGTGAC CACCTAACAT CATTCTCCAT CCTCATGTCC
    CCCGACTCTC CGTACCCTGG TTCCCTCCTG GGAATACTAC TGGATATCAT TTCCTACATT
    GGGTTATGCT TTTCCATCTT GAGCTTAGCA GCCTGTCTAG TGGTGGAAGC TGTGGTGTGG
    AAATCAGTGA CCAAGAATCG GACTTCCTAT ATGCGCCACA CCTGCATAGT GAACATTGCA
    GCCTCTCTTC TGGTTGCTGA CATCTGGTTC ATTGTAGCTG CTGCCATCCA CGACCATTTT
    TACCCACTCA ATGAGACAGC CTGTGTGGCC GCCACCTTTT TCATCCACTT CTTCTACCTC
    AGCGTCTTTT TCTGGATGCT GACTCTGGGC CTCATGCTCT TCTACCGCCT GGTTTTCATC
    CTGCATGACA CAAGCAAGTC CATTCAGAAG ACTATTGCAT TTTCTCTTGG CTATGGCTGC
    CCTCTTGTCA TCTCAGTCAT CACAGTGGGG GCCACCCAAC CCCAAGAAGT CTACACCAGG
    AAGAATGCCT GCTGGCTCAA CTGGGAGGAT ACCAAGGCCC TGCTGGCCTT TGTCATCCCA
    GCACTGATCA TCGTGGTGGT GAACATGACC ATCACGGTTG TGGTCATCTC CAAGATCCTG
    AGACCCTCCA TTGGAGACAA GCCAAGCAAG CAGGAGAAGA GTAGCTTGTT TCAGATCAGC
    AAGAGCATTG GGGTCCTAAC ACCACTCTTG GGGCTCACCT GGGGTTTTGG TCTTGCCACT
    GTGTTCCAAG GAAGCAATGC TGTGTTCCAT ATTATATTTA CACTCCTCAA TGCCTTTCAG
    GGATTATTCA TTTTGCTCTT TGGATGCCTC TGGGATCAGA AGGTACAGGA AGCCTTACTA
    AAGAAGTTTT CACTGTCAAG ATGGTCTTCT CAGCACTCAA AGTCAACATC CCTAGGTTCA
    TCTACACCAG TATTTTCTAT GAGTTCTCCA ATATCAAGAA GATTTAACAA TTTATTTGGA
    AAAACAGGAA CGTACAATGT TTCCACCCCA GAAACAACCA GCTCATCCCT GGAAAACACA
    TCCAGTGCTT ACTCCTTGCT GAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe15372
    Clone ID Related Accession (Same CDS sequence) XM_005627443.2 , XM_005627443.3
    Accession Version XM_005627443.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4164bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product adhesion G protein-coupled receptor F5 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005627443.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAATCCC CAAGGAGAAC CACATTGTGC TTCATGCTTA TTGTGATTTA TTTTTCCCAA 
    GCTACACTGA ACTTGAATTT AGAATCTATT GTTCATCCTT TGATTCTCCA TGAACATGAA
    CTAACAGAGA AGGAGCCACT AAGGGAAAAA CGAGCAGTTG CCTCCAGCAG CCCAGTACCT
    GCAGAGTACA CTGTTGATGT GGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAGCCTATC
    AAAGCTTATG TGAACAGCCT CAGTTTCCCA ATTCCAGGGA ACAGCACAGA CCTGGCCACC
    AACATTTTAA GCATTGAGGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CGGGCTATAG GTGGCCTCAG GAGAAGTGCC TCCACAATCC CACTTGTCAA
    GAGCATGACA GCTCCCTTGC AGGGCATCGT TGCAGTTGCC TGAAAGGACT ACCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGATGTT ACTTTAAGAA TGTCAGTCAG ACTCAATGTG
    GGCTTTCAAG AGGACCTCAA GAATTCTTCC TCTGCTCTCT ATAGGTCCTA CAAGACTGAC
    CTGGAAACAG CGTTCTGGAA AGGTTACAGT ACTTTACCAG GCTTCAAATT GGTGACTGTG
    ACAGGGTTCA GGCCCGGAAG TGTGGTTGTG ACATATGAGG TGAAGACTAC AACACCATCA
    CCTCAATTGA TGCATAAAGC AAATGAGCAA GTGATGCAGA ACCTGAATCA GACCTACAGA
    ATGGACTACA GTTCCTTTCA AGCCGTTACT AGCAATGAAA CTAAGTTCAC CATCATACCA
    GAAATCATTT TTGAAGGGGA TACGGTAAAC ATGATGTGTG AAACCGACGT TTTGTCCTCT
    AACGTGTCCT GGCACCATGT TGAAAGGCAC TCTGACATCC AGAACAGCAG CAGATTCTCA
    GTTTACACCA CCGTGCTGAA CAACATGACC TCAGTCTCCC GCCTCACGAT TTACAACATC
    ACCCAGCGGG ATGCAGGTGA ATATATTTGC AAGCTGACAT TAGATATTTT TGAATATGAG
    TCCAGAAAAA AAATAGATGT CACGCCCATC CGAATTCTGG CAACTGAAGA AATGAAGGTG
    ACGTGCGACA ACAATCCTAT ATCTTTGAAC TGCTGCAGTG AGATTAATGC TAACTGGAGC
    ACAATAGAAT GGAAGCAGGA GGGGAAAATA AACATTCCAG GCAACCCTGA AACTGACCTA
    GAGTCCAGCT GCAGCAGATA CACCCTCAAG GCAGATGGAA CCCAGTGTCC GAGTGGGTCG
    GCTGGAACCA CGGGCATCTT TGTGTGTGAG TTCATCAGCA CCTACGGAGC CAGAGGCAGT
    AAGAGCATAG AAGTGACGTT CATCTCTGTG GGCGATAATT CCAGCCACAA AGGGGAAGTG
    CACGAGATCC TCAGGCATGA TACGGAAATG AGTACAGTTC AGAGCACCAC CGGGAACAGC
    CTAGAAACAA GTAAAGCCCA GGAGCAGCAA GCCAACCTAA CAATAACCCC GGACCCAATT
    TCTGTTTCTG AGGGACAAAA CTTTTCCATA AAGTGCATCA GCGATGTGAG TAACTATGAT
    GAGGTATACT GGAACACGTC TGCTGGAATT AAAATATACC AAAAGTTTTA TACCACGAGG
    AGGTTTTCTG ATGGAGCAGA GTCAGTGCTG ACAGTGAAGA CTGTGACCCG GGAGTGGAAC
    GGAACCTATC ACTGCATATT TAGATACAAG AATTCATACA GTGTCGTAAT GAAAGATGTG
    ACCGTTTACC CACTGCCTCT AGAGCCAAAC ATCATGGTTG ATCCTCTGGA GGCTACAGTT
    CCCTGCAAGG GTTCCCATCA CTTCAGATGC TGCATTGACG AGGATGAGAA CTACAGAGTG
    ACTTTCCAAG TGGATTCCTT ATCCTTTCCT GCAGAAAAAG AAGTTAATGG CAAGCAAGTG
    TGCTACAAAT ACAAGTTCAT GGCAAGGACA TCAGTTTCCT GGTGTCTAAA AAAGCTGGAC
    ATATTTTGTC ACTTTACCAA TGCTGCTAAT AATTCAGTCC GGAGCCCATC TATGAAGCTT
    AATGTGGTTC CTGGAAAGAA CATCATGTGC CGAGATCCCG TAATAGGTGT TGGGGAGCCT
    GGAAAAGTCA TCCAGAAACT CTGCCATTTC TCAAAAGTAC CAAACATCTC TGGAGGTCCT
    ACTGGAGGGA TCATAACTTA CAAATGTGTA GGCTCCCAAT GGGAGGTGAA GAAAAATGAC
    TGCATCTCTG CCCCAATAAA TAGTCTGCTC CAGCTGGCCA AGGCTTTGAC CAAGAGCCCC
    ACTCAGGATA AGAAGCTCCC AACATACCTA AAGGATCTTT CTATTAGCAC AGGCAAGGTA
    AAACATGAAG TCAGCTCATT TCCTGGGAGC CTGGGAGCCA TCGTTAACAT CCTTGATTTG
    CTTTCAACAG TTACAACCCA AGTGAATTCA GAGATGATGA TGAACGTTCT CTCTACGGTT
    AACACTGTCC TCAGCAAGCC CATGTTGAGT ACCTGGAAGG TGTCCCAACA GCAACAGACC
    AACCAGAGCT CACAGCTACT GGATTCAGTA GAAAGATTTT CCCGAGCATT ACAGACAGAA
    GATAGCACCT TTTTATCCAT CAACCAAACT AACGTGCAGA TGAGGAGCAT GGTAATAAAA
    ACTGGCCATC CAAAAACCTA CAAACAGAGC TTTGTTTTCT CAGACTTGGA CCTCTGGGGC
    AATGTGGCCA TTGACAAGTG CGAGGTGGGA AGCCTGCAGC AAGATTCATC TATTGTCACC
    GTGGCTTTCC CAACTCTCCA AACCATCCTC ACTCAGGATG TTCAAGGAAA GAATTTTGCC
    AACAGCTTAG TGATGACAAC CACTGTTAGC CACAACATAA CCATGCCATT TAGGATTTCA
    ATGACTTTTA AGAACATCAA TCCTTCAGGT GGGGTACCAC AATGTGTCTA CTGGAATTTC
    AACCTCGCCA ACCACACAGG GGGTTGGGAT AGTAGTGGGT GTTATGTGCA CAATGTCGAT
    GGGGACAGTG TCTCCTGCAT CTGTGACCAC CTAACATCAT TCTCCATCCT CATGTCCCCC
    GACTCTCCGT ACCCTGGTTC CCTCCTGGGA ATACTACTGG ATATCATTTC CTACATTGGG
    TTATGCTTTT CCATCTTGAG CTTAGCAGCC TGTCTAGTGG TGGAAGCTGT GGTGTGGAAA
    TCAGTGACCA AGAATCGGAC TTCCTATATG CGCCACACCT GCATAGTGAA CATTGCAGCC
    TCTCTTCTGG TTGCTGACAT CTGGTTCATT GTAGCTGCTG CCATCCACGA CCATTTTTAC
    CCACTCAATG AGACAGCCTG TGTGGCCGCC ACCTTTTTCA TCCACTTCTT CTACCTCAGC
    GTCTTTTTCT GGATGCTGAC TCTGGGCCTC ATGCTCTTCT ACCGCCTGGT TTTCATCCTG
    CATGACACAA GCAAGTCCAT TCAGAAGACT ATTGCATTTT CTCTTGGCTA TGGCTGCCCT
    CTTGTCATCT CAGTCATCAC AGTGGGGGCC ACCCAACCCC AAGAAGTCTA CACCAGGAAG
    AATGCCTGCT GGCTCAACTG GGAGGATACC AAGGCCCTGC TGGCCTTTGT CATCCCAGCA
    CTGATCATCG TGGTGGTGAA CATGACCATC ACGGTTGTGG TCATCTCCAA GATCCTGAGA
    CCCTCCATTG GAGACAAGCC AAGCAAGCAG GAGAAGAGTA GCTTGTTTCA GATCAGCAAG
    AGCATTGGGG TCCTAACACC ACTCTTGGGG CTCACCTGGG GTTTTGGTCT TGCCACTGTG
    TTCCAAGGAA GCAATGCTGT GTTCCATATT ATATTTACAC TCCTCAATGC CTTTCAGGGA
    TTATTCATTT TGCTCTTTGG ATGCCTCTGG GATCAGAAGG TACAGGAAGC CTTACTAAAG
    AAGTTTTCAC TGTCAAGATG GTCTTCTCAG CACTCAAAGT CAACATCCCT AGGTTCATCT
    ACACCAGTAT TTTCTATGAG TTCTCCAATA TCAAGAAGAT TTAACAATTT ATTTGGAAAA
    ACAGGAACGT ACAATGTTTC CACCCCAGAA ACAACCAGCT CATCCCTGGA AAACACATCC
    AGTGCTTACT CCTTGCTGAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005627500.1
    CDS397..4560
    Translation

    Target ORF information:

    RefSeq Version XM_005627443.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005627443.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAATCCC CAAGGAGAAC CACATTGTGC TTCATGCTTA TTGTGATTTA TTTTTCCCAA 
    GCTACACTGA ACTTGAATTT AGAATCTATT GTTCATCCTT TGATTCTCCA TGAACATGAA
    CTAACAGAGA AGGAGCCACT AAGGGAAAAA CGAGCAGTTG CCTCCAGCAG CCCAGTACCT
    GCAGAGTACA CTGTTGATGT GGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAGCCTATC
    AAAGCTTATG TGAACAGCCT CAGTTTCCCA ATTCCAGGGA ACAGCACAGA CCTGGCCACC
    AACATTTTAA GCATTGAGGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CGGGCTATAG GTGGCCTCAG GAGAAGTGCC TCCACAATCC CACTTGTCAA
    GAGCATGACA GCTCCCTTGC AGGGCATCGT TGCAGTTGCC TGAAAGGACT ACCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGATGTT ACTTTAAGAA TGTCAGTCAG ACTCAATGTG
    GGCTTTCAAG AGGACCTCAA GAATTCTTCC TCTGCTCTCT ATAGGTCCTA CAAGACTGAC
    CTGGAAACAG CGTTCTGGAA AGGTTACAGT ACTTTACCAG GCTTCAAATT GGTGACTGTG
    ACAGGGTTCA GGCCCGGAAG TGTGGTTGTG ACATATGAGG TGAAGACTAC AACACCATCA
    CCTCAATTGA TGCATAAAGC AAATGAGCAA GTGATGCAGA ACCTGAATCA GACCTACAGA
    ATGGACTACA GTTCCTTTCA AGCCGTTACT AGCAATGAAA CTAAGTTCAC CATCATACCA
    GAAATCATTT TTGAAGGGGA TACGGTAAAC ATGATGTGTG AAACCGACGT TTTGTCCTCT
    AACGTGTCCT GGCACCATGT TGAAAGGCAC TCTGACATCC AGAACAGCAG CAGATTCTCA
    GTTTACACCA CCGTGCTGAA CAACATGACC TCAGTCTCCC GCCTCACGAT TTACAACATC
    ACCCAGCGGG ATGCAGGTGA ATATATTTGC AAGCTGACAT TAGATATTTT TGAATATGAG
    TCCAGAAAAA AAATAGATGT CACGCCCATC CGAATTCTGG CAACTGAAGA AATGAAGGTG
    ACGTGCGACA ACAATCCTAT ATCTTTGAAC TGCTGCAGTG AGATTAATGC TAACTGGAGC
    ACAATAGAAT GGAAGCAGGA GGGGAAAATA AACATTCCAG GCAACCCTGA AACTGACCTA
    GAGTCCAGCT GCAGCAGATA CACCCTCAAG GCAGATGGAA CCCAGTGTCC GAGTGGGTCG
    GCTGGAACCA CGGGCATCTT TGTGTGTGAG TTCATCAGCA CCTACGGAGC CAGAGGCAGT
    AAGAGCATAG AAGTGACGTT CATCTCTGTG GGCGATAATT CCAGCCACAA AGGGGAAGTG
    CACGAGATCC TCAGGCATGA TACGGAAATG AGTACAGTTC AGAGCACCAC CGGGAACAGC
    CTAGAAACAA GTAAAGCCCA GGAGCAGCAA GCCAACCTAA CAATAACCCC GGACCCAATT
    TCTGTTTCTG AGGGACAAAA CTTTTCCATA AAGTGCATCA GCGATGTGAG TAACTATGAT
    GAGGTATACT GGAACACGTC TGCTGGAATT AAAATATACC AAAAGTTTTA TACCACGAGG
    AGGTTTTCTG ATGGAGCAGA GTCAGTGCTG ACAGTGAAGA CTGTGACCCG GGAGTGGAAC
    GGAACCTATC ACTGCATATT TAGATACAAG AATTCATACA GTGTCGTAAT GAAAGATGTG
    ACCGTTTACC CACTGCCTCT AGAGCCAAAC ATCATGGTTG ATCCTCTGGA GGCTACAGTT
    CCCTGCAAGG GTTCCCATCA CTTCAGATGC TGCATTGACG AGGATGAGAA CTACAGAGTG
    ACTTTCCAAG TGGATTCCTT ATCCTTTCCT GCAGAAAAAG AAGTTAATGG CAAGCAAGTG
    TGCTACAAAT ACAAGTTCAT GGCAAGGACA TCAGTTTCCT GGTGTCTAAA AAAGCTGGAC
    ATATTTTGTC ACTTTACCAA TGCTGCTAAT AATTCAGTCC GGAGCCCATC TATGAAGCTT
    AATGTGGTTC CTGGAAAGAA CATCATGTGC CGAGATCCCG TAATAGGTGT TGGGGAGCCT
    GGAAAAGTCA TCCAGAAACT CTGCCATTTC TCAAAAGTAC CAAACATCTC TGGAGGTCCT
    ACTGGAGGGA TCATAACTTA CAAATGTGTA GGCTCCCAAT GGGAGGTGAA GAAAAATGAC
    TGCATCTCTG CCCCAATAAA TAGTCTGCTC CAGCTGGCCA AGGCTTTGAC CAAGAGCCCC
    ACTCAGGATA AGAAGCTCCC AACATACCTA AAGGATCTTT CTATTAGCAC AGGCAAGGTA
    AAACATGAAG TCAGCTCATT TCCTGGGAGC CTGGGAGCCA TCGTTAACAT CCTTGATTTG
    CTTTCAACAG TTACAACCCA AGTGAATTCA GAGATGATGA TGAACGTTCT CTCTACGGTT
    AACACTGTCC TCAGCAAGCC CATGTTGAGT ACCTGGAAGG TGTCCCAACA GCAACAGACC
    AACCAGAGCT CACAGCTACT GGATTCAGTA GAAAGATTTT CCCGAGCATT ACAGACAGAA
    GATAGCACCT TTTTATCCAT CAACCAAACT AACGTGCAGA TGAGGAGCAT GGTAATAAAA
    ACTGGCCATC CAAAAACCTA CAAACAGAGC TTTGTTTTCT CAGACTTGGA CCTCTGGGGC
    AATGTGGCCA TTGACAAGTG CGAGGTGGGA AGCCTGCAGC AAGATTCATC TATTGTCACC
    GTGGCTTTCC CAACTCTCCA AACCATCCTC ACTCAGGATG TTCAAGGAAA GAATTTTGCC
    AACAGCTTAG TGATGACAAC CACTGTTAGC CACAACATAA CCATGCCATT TAGGATTTCA
    ATGACTTTTA AGAACATCAA TCCTTCAGGT GGGGTACCAC AATGTGTCTA CTGGAATTTC
    AACCTCGCCA ACCACACAGG GGGTTGGGAT AGTAGTGGGT GTTATGTGCA CAATGTCGAT
    GGGGACAGTG TCTCCTGCAT CTGTGACCAC CTAACATCAT TCTCCATCCT CATGTCCCCC
    GACTCTCCGT ACCCTGGTTC CCTCCTGGGA ATACTACTGG ATATCATTTC CTACATTGGG
    TTATGCTTTT CCATCTTGAG CTTAGCAGCC TGTCTAGTGG TGGAAGCTGT GGTGTGGAAA
    TCAGTGACCA AGAATCGGAC TTCCTATATG CGCCACACCT GCATAGTGAA CATTGCAGCC
    TCTCTTCTGG TTGCTGACAT CTGGTTCATT GTAGCTGCTG CCATCCACGA CCATTTTTAC
    CCACTCAATG AGACAGCCTG TGTGGCCGCC ACCTTTTTCA TCCACTTCTT CTACCTCAGC
    GTCTTTTTCT GGATGCTGAC TCTGGGCCTC ATGCTCTTCT ACCGCCTGGT TTTCATCCTG
    CATGACACAA GCAAGTCCAT TCAGAAGACT ATTGCATTTT CTCTTGGCTA TGGCTGCCCT
    CTTGTCATCT CAGTCATCAC AGTGGGGGCC ACCCAACCCC AAGAAGTCTA CACCAGGAAG
    AATGCCTGCT GGCTCAACTG GGAGGATACC AAGGCCCTGC TGGCCTTTGT CATCCCAGCA
    CTGATCATCG TGGTGGTGAA CATGACCATC ACGGTTGTGG TCATCTCCAA GATCCTGAGA
    CCCTCCATTG GAGACAAGCC AAGCAAGCAG GAGAAGAGTA GCTTGTTTCA GATCAGCAAG
    AGCATTGGGG TCCTAACACC ACTCTTGGGG CTCACCTGGG GTTTTGGTCT TGCCACTGTG
    TTCCAAGGAA GCAATGCTGT GTTCCATATT ATATTTACAC TCCTCAATGC CTTTCAGGGA
    TTATTCATTT TGCTCTTTGG ATGCCTCTGG GATCAGAAGG TACAGGAAGC CTTACTAAAG
    AAGTTTTCAC TGTCAAGATG GTCTTCTCAG CACTCAAAGT CAACATCCCT AGGTTCATCT
    ACACCAGTAT TTTCTATGAG TTCTCCAATA TCAAGAAGAT TTAACAATTT ATTTGGAAAA
    ACAGGAACGT ACAATGTTTC CACCCCAGAA ACAACCAGCT CATCCCTGGA AAACACATCC
    AGTGCTTACT CCTTGCTGAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe15372
    Clone ID Related Accession (Same CDS sequence) XM_005627443.2 , XM_005627443.3
    Accession Version XM_005627443.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4164bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product adhesion G protein-coupled receptor F5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005627443.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAATCCC CAAGGAGAAC CACATTGTGC TTCATGCTTA TTGTGATTTA TTTTTCCCAA 
    GCTACACTGA ACTTGAATTT AGAATCTATT GTTCATCCTT TGATTCTCCA TGAACATGAA
    CTAACAGAGA AGGAGCCACT AAGGGAAAAA CGAGCAGTTG CCTCCAGCAG CCCAGTACCT
    GCAGAGTACA CTGTTGATGT GGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAGCCTATC
    AAAGCTTATG TGAACAGCCT CAGTTTCCCA ATTCCAGGGA ACAGCACAGA CCTGGCCACC
    AACATTTTAA GCATTGAGGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CGGGCTATAG GTGGCCTCAG GAGAAGTGCC TCCACAATCC CACTTGTCAA
    GAGCATGACA GCTCCCTTGC AGGGCATCGT TGCAGTTGCC TGAAAGGACT ACCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGATGTT ACTTTAAGAA TGTCAGTCAG ACTCAATGTG
    GGCTTTCAAG AGGACCTCAA GAATTCTTCC TCTGCTCTCT ATAGGTCCTA CAAGACTGAC
    CTGGAAACAG CGTTCTGGAA AGGTTACAGT ACTTTACCAG GCTTCAAATT GGTGACTGTG
    ACAGGGTTCA GGCCCGGAAG TGTGGTTGTG ACATATGAGG TGAAGACTAC AACACCATCA
    CCTCAATTGA TGCATAAAGC AAATGAGCAA GTGATGCAGA ACCTGAATCA GACCTACAGA
    ATGGACTACA GTTCCTTTCA AGCCGTTACT AGCAATGAAA CTAAGTTCAC CATCATACCA
    GAAATCATTT TTGAAGGGGA TACGGTAAAC ATGATGTGTG AAACCGACGT TTTGTCCTCT
    AACGTGTCCT GGCACCATGT TGAAAGGCAC TCTGACATCC AGAACAGCAG CAGATTCTCA
    GTTTACACCA CCGTGCTGAA CAACATGACC TCAGTCTCCC GCCTCACGAT TTACAACATC
    ACCCAGCGGG ATGCAGGTGA ATATATTTGC AAGCTGACAT TAGATATTTT TGAATATGAG
    TCCAGAAAAA AAATAGATGT CACGCCCATC CGAATTCTGG CAACTGAAGA AATGAAGGTG
    ACGTGCGACA ACAATCCTAT ATCTTTGAAC TGCTGCAGTG AGATTAATGC TAACTGGAGC
    ACAATAGAAT GGAAGCAGGA GGGGAAAATA AACATTCCAG GCAACCCTGA AACTGACCTA
    GAGTCCAGCT GCAGCAGATA CACCCTCAAG GCAGATGGAA CCCAGTGTCC GAGTGGGTCG
    GCTGGAACCA CGGGCATCTT TGTGTGTGAG TTCATCAGCA CCTACGGAGC CAGAGGCAGT
    AAGAGCATAG AAGTGACGTT CATCTCTGTG GGCGATAATT CCAGCCACAA AGGGGAAGTG
    CACGAGATCC TCAGGCATGA TACGGAAATG AGTACAGTTC AGAGCACCAC CGGGAACAGC
    CTAGAAACAA GTAAAGCCCA GGAGCAGCAA GCCAACCTAA CAATAACCCC GGACCCAATT
    TCTGTTTCTG AGGGACAAAA CTTTTCCATA AAGTGCATCA GCGATGTGAG TAACTATGAT
    GAGGTATACT GGAACACGTC TGCTGGAATT AAAATATACC AAAAGTTTTA TACCACGAGG
    AGGTTTTCTG ATGGAGCAGA GTCAGTGCTG ACAGTGAAGA CTGTGACCCG GGAGTGGAAC
    GGAACCTATC ACTGCATATT TAGATACAAG AATTCATACA GTGTCGTAAT GAAAGATGTG
    ACCGTTTACC CACTGCCTCT AGAGCCAAAC ATCATGGTTG ATCCTCTGGA GGCTACAGTT
    CCCTGCAAGG GTTCCCATCA CTTCAGATGC TGCATTGACG AGGATGAGAA CTACAGAGTG
    ACTTTCCAAG TGGATTCCTT ATCCTTTCCT GCAGAAAAAG AAGTTAATGG CAAGCAAGTG
    TGCTACAAAT ACAAGTTCAT GGCAAGGACA TCAGTTTCCT GGTGTCTAAA AAAGCTGGAC
    ATATTTTGTC ACTTTACCAA TGCTGCTAAT AATTCAGTCC GGAGCCCATC TATGAAGCTT
    AATGTGGTTC CTGGAAAGAA CATCATGTGC CGAGATCCCG TAATAGGTGT TGGGGAGCCT
    GGAAAAGTCA TCCAGAAACT CTGCCATTTC TCAAAAGTAC CAAACATCTC TGGAGGTCCT
    ACTGGAGGGA TCATAACTTA CAAATGTGTA GGCTCCCAAT GGGAGGTGAA GAAAAATGAC
    TGCATCTCTG CCCCAATAAA TAGTCTGCTC CAGCTGGCCA AGGCTTTGAC CAAGAGCCCC
    ACTCAGGATA AGAAGCTCCC AACATACCTA AAGGATCTTT CTATTAGCAC AGGCAAGGTA
    AAACATGAAG TCAGCTCATT TCCTGGGAGC CTGGGAGCCA TCGTTAACAT CCTTGATTTG
    CTTTCAACAG TTACAACCCA AGTGAATTCA GAGATGATGA TGAACGTTCT CTCTACGGTT
    AACACTGTCC TCAGCAAGCC CATGTTGAGT ACCTGGAAGG TGTCCCAACA GCAACAGACC
    AACCAGAGCT CACAGCTACT GGATTCAGTA GAAAGATTTT CCCGAGCATT ACAGACAGAA
    GATAGCACCT TTTTATCCAT CAACCAAACT AACGTGCAGA TGAGGAGCAT GGTAATAAAA
    ACTGGCCATC CAAAAACCTA CAAACAGAGC TTTGTTTTCT CAGACTTGGA CCTCTGGGGC
    AATGTGGCCA TTGACAAGTG CGAGGTGGGA AGCCTGCAGC AAGATTCATC TATTGTCACC
    GTGGCTTTCC CAACTCTCCA AACCATCCTC ACTCAGGATG TTCAAGGAAA GAATTTTGCC
    AACAGCTTAG TGATGACAAC CACTGTTAGC CACAACATAA CCATGCCATT TAGGATTTCA
    ATGACTTTTA AGAACATCAA TCCTTCAGGT GGGGTACCAC AATGTGTCTA CTGGAATTTC
    AACCTCGCCA ACCACACAGG GGGTTGGGAT AGTAGTGGGT GTTATGTGCA CAATGTCGAT
    GGGGACAGTG TCTCCTGCAT CTGTGACCAC CTAACATCAT TCTCCATCCT CATGTCCCCC
    GACTCTCCGT ACCCTGGTTC CCTCCTGGGA ATACTACTGG ATATCATTTC CTACATTGGG
    TTATGCTTTT CCATCTTGAG CTTAGCAGCC TGTCTAGTGG TGGAAGCTGT GGTGTGGAAA
    TCAGTGACCA AGAATCGGAC TTCCTATATG CGCCACACCT GCATAGTGAA CATTGCAGCC
    TCTCTTCTGG TTGCTGACAT CTGGTTCATT GTAGCTGCTG CCATCCACGA CCATTTTTAC
    CCACTCAATG AGACAGCCTG TGTGGCCGCC ACCTTTTTCA TCCACTTCTT CTACCTCAGC
    GTCTTTTTCT GGATGCTGAC TCTGGGCCTC ATGCTCTTCT ACCGCCTGGT TTTCATCCTG
    CATGACACAA GCAAGTCCAT TCAGAAGACT ATTGCATTTT CTCTTGGCTA TGGCTGCCCT
    CTTGTCATCT CAGTCATCAC AGTGGGGGCC ACCCAACCCC AAGAAGTCTA CACCAGGAAG
    AATGCCTGCT GGCTCAACTG GGAGGATACC AAGGCCCTGC TGGCCTTTGT CATCCCAGCA
    CTGATCATCG TGGTGGTGAA CATGACCATC ACGGTTGTGG TCATCTCCAA GATCCTGAGA
    CCCTCCATTG GAGACAAGCC AAGCAAGCAG GAGAAGAGTA GCTTGTTTCA GATCAGCAAG
    AGCATTGGGG TCCTAACACC ACTCTTGGGG CTCACCTGGG GTTTTGGTCT TGCCACTGTG
    TTCCAAGGAA GCAATGCTGT GTTCCATATT ATATTTACAC TCCTCAATGC CTTTCAGGGA
    TTATTCATTT TGCTCTTTGG ATGCCTCTGG GATCAGAAGG TACAGGAAGC CTTACTAAAG
    AAGTTTTCAC TGTCAAGATG GTCTTCTCAG CACTCAAAGT CAACATCCCT AGGTTCATCT
    ACACCAGTAT TTTCTATGAG TTCTCCAATA TCAAGAAGAT TTAACAATTT ATTTGGAAAA
    ACAGGAACGT ACAATGTTTC CACCCCAGAA ACAACCAGCT CATCCCTGGA AAACACATCC
    AGTGCTTACT CCTTGCTGAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005627500.1
    CDS415..4578
    Translation

    Target ORF information:

    RefSeq Version XM_005627443.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005627443.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAATCCC CAAGGAGAAC CACATTGTGC TTCATGCTTA TTGTGATTTA TTTTTCCCAA 
    GCTACACTGA ACTTGAATTT AGAATCTATT GTTCATCCTT TGATTCTCCA TGAACATGAA
    CTAACAGAGA AGGAGCCACT AAGGGAAAAA CGAGCAGTTG CCTCCAGCAG CCCAGTACCT
    GCAGAGTACA CTGTTGATGT GGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAGCCTATC
    AAAGCTTATG TGAACAGCCT CAGTTTCCCA ATTCCAGGGA ACAGCACAGA CCTGGCCACC
    AACATTTTAA GCATTGAGGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CGGGCTATAG GTGGCCTCAG GAGAAGTGCC TCCACAATCC CACTTGTCAA
    GAGCATGACA GCTCCCTTGC AGGGCATCGT TGCAGTTGCC TGAAAGGACT ACCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGATGTT ACTTTAAGAA TGTCAGTCAG ACTCAATGTG
    GGCTTTCAAG AGGACCTCAA GAATTCTTCC TCTGCTCTCT ATAGGTCCTA CAAGACTGAC
    CTGGAAACAG CGTTCTGGAA AGGTTACAGT ACTTTACCAG GCTTCAAATT GGTGACTGTG
    ACAGGGTTCA GGCCCGGAAG TGTGGTTGTG ACATATGAGG TGAAGACTAC AACACCATCA
    CCTCAATTGA TGCATAAAGC AAATGAGCAA GTGATGCAGA ACCTGAATCA GACCTACAGA
    ATGGACTACA GTTCCTTTCA AGCCGTTACT AGCAATGAAA CTAAGTTCAC CATCATACCA
    GAAATCATTT TTGAAGGGGA TACGGTAAAC ATGATGTGTG AAACCGACGT TTTGTCCTCT
    AACGTGTCCT GGCACCATGT TGAAAGGCAC TCTGACATCC AGAACAGCAG CAGATTCTCA
    GTTTACACCA CCGTGCTGAA CAACATGACC TCAGTCTCCC GCCTCACGAT TTACAACATC
    ACCCAGCGGG ATGCAGGTGA ATATATTTGC AAGCTGACAT TAGATATTTT TGAATATGAG
    TCCAGAAAAA AAATAGATGT CACGCCCATC CGAATTCTGG CAACTGAAGA AATGAAGGTG
    ACGTGCGACA ACAATCCTAT ATCTTTGAAC TGCTGCAGTG AGATTAATGC TAACTGGAGC
    ACAATAGAAT GGAAGCAGGA GGGGAAAATA AACATTCCAG GCAACCCTGA AACTGACCTA
    GAGTCCAGCT GCAGCAGATA CACCCTCAAG GCAGATGGAA CCCAGTGTCC GAGTGGGTCG
    GCTGGAACCA CGGGCATCTT TGTGTGTGAG TTCATCAGCA CCTACGGAGC CAGAGGCAGT
    AAGAGCATAG AAGTGACGTT CATCTCTGTG GGCGATAATT CCAGCCACAA AGGGGAAGTG
    CACGAGATCC TCAGGCATGA TACGGAAATG AGTACAGTTC AGAGCACCAC CGGGAACAGC
    CTAGAAACAA GTAAAGCCCA GGAGCAGCAA GCCAACCTAA CAATAACCCC GGACCCAATT
    TCTGTTTCTG AGGGACAAAA CTTTTCCATA AAGTGCATCA GCGATGTGAG TAACTATGAT
    GAGGTATACT GGAACACGTC TGCTGGAATT AAAATATACC AAAAGTTTTA TACCACGAGG
    AGGTTTTCTG ATGGAGCAGA GTCAGTGCTG ACAGTGAAGA CTGTGACCCG GGAGTGGAAC
    GGAACCTATC ACTGCATATT TAGATACAAG AATTCATACA GTGTCGTAAT GAAAGATGTG
    ACCGTTTACC CACTGCCTCT AGAGCCAAAC ATCATGGTTG ATCCTCTGGA GGCTACAGTT
    CCCTGCAAGG GTTCCCATCA CTTCAGATGC TGCATTGACG AGGATGAGAA CTACAGAGTG
    ACTTTCCAAG TGGATTCCTT ATCCTTTCCT GCAGAAAAAG AAGTTAATGG CAAGCAAGTG
    TGCTACAAAT ACAAGTTCAT GGCAAGGACA TCAGTTTCCT GGTGTCTAAA AAAGCTGGAC
    ATATTTTGTC ACTTTACCAA TGCTGCTAAT AATTCAGTCC GGAGCCCATC TATGAAGCTT
    AATGTGGTTC CTGGAAAGAA CATCATGTGC CGAGATCCCG TAATAGGTGT TGGGGAGCCT
    GGAAAAGTCA TCCAGAAACT CTGCCATTTC TCAAAAGTAC CAAACATCTC TGGAGGTCCT
    ACTGGAGGGA TCATAACTTA CAAATGTGTA GGCTCCCAAT GGGAGGTGAA GAAAAATGAC
    TGCATCTCTG CCCCAATAAA TAGTCTGCTC CAGCTGGCCA AGGCTTTGAC CAAGAGCCCC
    ACTCAGGATA AGAAGCTCCC AACATACCTA AAGGATCTTT CTATTAGCAC AGGCAAGGTA
    AAACATGAAG TCAGCTCATT TCCTGGGAGC CTGGGAGCCA TCGTTAACAT CCTTGATTTG
    CTTTCAACAG TTACAACCCA AGTGAATTCA GAGATGATGA TGAACGTTCT CTCTACGGTT
    AACACTGTCC TCAGCAAGCC CATGTTGAGT ACCTGGAAGG TGTCCCAACA GCAACAGACC
    AACCAGAGCT CACAGCTACT GGATTCAGTA GAAAGATTTT CCCGAGCATT ACAGACAGAA
    GATAGCACCT TTTTATCCAT CAACCAAACT AACGTGCAGA TGAGGAGCAT GGTAATAAAA
    ACTGGCCATC CAAAAACCTA CAAACAGAGC TTTGTTTTCT CAGACTTGGA CCTCTGGGGC
    AATGTGGCCA TTGACAAGTG CGAGGTGGGA AGCCTGCAGC AAGATTCATC TATTGTCACC
    GTGGCTTTCC CAACTCTCCA AACCATCCTC ACTCAGGATG TTCAAGGAAA GAATTTTGCC
    AACAGCTTAG TGATGACAAC CACTGTTAGC CACAACATAA CCATGCCATT TAGGATTTCA
    ATGACTTTTA AGAACATCAA TCCTTCAGGT GGGGTACCAC AATGTGTCTA CTGGAATTTC
    AACCTCGCCA ACCACACAGG GGGTTGGGAT AGTAGTGGGT GTTATGTGCA CAATGTCGAT
    GGGGACAGTG TCTCCTGCAT CTGTGACCAC CTAACATCAT TCTCCATCCT CATGTCCCCC
    GACTCTCCGT ACCCTGGTTC CCTCCTGGGA ATACTACTGG ATATCATTTC CTACATTGGG
    TTATGCTTTT CCATCTTGAG CTTAGCAGCC TGTCTAGTGG TGGAAGCTGT GGTGTGGAAA
    TCAGTGACCA AGAATCGGAC TTCCTATATG CGCCACACCT GCATAGTGAA CATTGCAGCC
    TCTCTTCTGG TTGCTGACAT CTGGTTCATT GTAGCTGCTG CCATCCACGA CCATTTTTAC
    CCACTCAATG AGACAGCCTG TGTGGCCGCC ACCTTTTTCA TCCACTTCTT CTACCTCAGC
    GTCTTTTTCT GGATGCTGAC TCTGGGCCTC ATGCTCTTCT ACCGCCTGGT TTTCATCCTG
    CATGACACAA GCAAGTCCAT TCAGAAGACT ATTGCATTTT CTCTTGGCTA TGGCTGCCCT
    CTTGTCATCT CAGTCATCAC AGTGGGGGCC ACCCAACCCC AAGAAGTCTA CACCAGGAAG
    AATGCCTGCT GGCTCAACTG GGAGGATACC AAGGCCCTGC TGGCCTTTGT CATCCCAGCA
    CTGATCATCG TGGTGGTGAA CATGACCATC ACGGTTGTGG TCATCTCCAA GATCCTGAGA
    CCCTCCATTG GAGACAAGCC AAGCAAGCAG GAGAAGAGTA GCTTGTTTCA GATCAGCAAG
    AGCATTGGGG TCCTAACACC ACTCTTGGGG CTCACCTGGG GTTTTGGTCT TGCCACTGTG
    TTCCAAGGAA GCAATGCTGT GTTCCATATT ATATTTACAC TCCTCAATGC CTTTCAGGGA
    TTATTCATTT TGCTCTTTGG ATGCCTCTGG GATCAGAAGG TACAGGAAGC CTTACTAAAG
    AAGTTTTCAC TGTCAAGATG GTCTTCTCAG CACTCAAAGT CAACATCCCT AGGTTCATCT
    ACACCAGTAT TTTCTATGAG TTCTCCAATA TCAAGAAGAT TTAACAATTT ATTTGGAAAA
    ACAGGAACGT ACAATGTTTC CACCCCAGAA ACAACCAGCT CATCCCTGGA AAACACATCC
    AGTGCTTACT CCTTGCTGAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe15373
    Clone ID Related Accession (Same CDS sequence) XM_005627444.2 , XM_005627444.3
    Accession Version XM_005627444.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4152bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product adhesion G protein-coupled receptor F5 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005627444.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAATCCC CAAGGAGAAC CACATTGTGC TTCATGCTTA TTGTGATTTA TTTTTCCCAA 
    GCTACACTGA ACTTGAATTT AGAATCTATT GTTCATCCTT TGATTCTCCA TGAACATGAA
    CTAACAGAGA AGGAGCCACT AAGGGAAAAA CGAGCAGTTG CCTCCAGCAG CCCAGTACCT
    GCAGAGTACA CTGTTGATGT GGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAGCCTATC
    AAAGCTTATG TGAACAGCCT CAGTTTCCCA ATTCCAGGGA ACAGCACAGA CCTGGCCACC
    AACATTTTAA GCATTGAGGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CGGGCTATAG GTGGCCTCAG GAGAAGTGCC TCCACAATCC CACTTGTCAA
    GAGCATGACA GCTCCCTTGC AGGGCATCGT TGCAGTTGCC TGAAAGGACT ACCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGCAGAT GTTACTTTAA GAATGTCAGT CAGACTCAAT
    GTGGGCTTTC AAGAGGACCT CAAGAATTCT TCCTCTGCTC TCTATAGGTC CTACAAGACT
    GACCTGGAAA CAGCGTTCTG GAAAGGTTAC AGTACTTTAC CAGGCTTCAA ATTGGTGACT
    GTGACAGGGT TCAGGCCCGG AAGTGTGGTT GTGACATATG AGGTGAAGAC TACAACACCA
    TCACCTCAAT TGATGCATAA AGCAAATGAG CAAGTGATGC AGAACCTGAA TCAGACCTAC
    AGAATGGACT ACAGTTCCTT TCAAGCCGTT ACTAGCAATG AAACTAAGTT CACCATCATA
    CCAGAAATCA TTTTTGAAGG GGATACGGTA AACATGATGT GTGAAACCGA CGTTTTGTCC
    TCTAACGTGT CCTGGCACCA TGTTGAAAGG CACTCTGACA TCCAGAACAG CAGCAGATTC
    TCAGTTTACA CCACCGTGCT GAACAACATG ACCTCAGTCT CCCGCCTCAC GATTTACAAC
    ATCACCCAGC GGGATGCAGG TGAATATATT TGCAAGCTGA CATTAGATAT TTTTGAATAT
    GAGTCCAGAA AAAAAATAGA TGTCACGCCC ATCCGAATTC TGGCAACTGA AGAAATGAAG
    GTGACGTGCG ACAACAATCC TATATCTTTG AACTGCTGCA GTGAGATTAA TGCTAACTGG
    AGCACAATAG AATGGAAGCA GGAGGGGAAA ATAAACATTC CAGGCAACCC TGAAACTGAC
    CTAGAGTCCA GCTGCAGCAG ATACACCCTC AAGGCAGATG GAACCCAGTG TCCGAGTGGG
    TCGGCTGGAA CCACGGGCAT CTTTGTGTGT GAGTTCATCA GCACCTACGG AGCCAGAGGC
    AGTAAGAGCA TAGAAGTGAC GTTCATCTCT GTGGGCGATA ATTCCAGCCA CAAAGGGGAA
    GTGCACGAGA TCCTCAGGCA TGATACGGAA ATGAGTACAG TTCAGAGCAC CACCGGGAAC
    AGCCTAGAAA CAAGTAAAGC CCAGGAGCAG CAAGCCAACC TAACAATAAC CCCGGACCCA
    ATTTCTGTTT CTGAGGGACA AAACTTTTCC ATAAAGTGCA TCAGCGATGT GAGTAACTAT
    GATGAGGTAT ACTGGAACAC GTCTGCTGGA ATTAAAATAT ACCAAAAGTT TTATACCACG
    AGGAGGTTTT CTGATGGAGC AGAGTCAGTG CTGACAGTGA AGACTGTGAC CCGGGAGTGG
    AACGGAACCT ATCACTGCAT ATTTAGATAC AAGAATTCAT ACAGTGTCGT AATGAAAGAT
    GTGACCGTTT ACCCACTGCC TCTAGAGCCA AACATCATGG TTGATCCTCT GGAGGCTACA
    GTTCCCTGCA AGGGTTCCCA TCACTTCAGA TGCTGCATTG ACGAGGATGA GAACTACAGA
    GTGACTTTCC AAGTGGATTC CTTATCCTTT CCTGCAGAAA AAGAAGTTAA TGGCAAGCAA
    GTGTGCTACA AATACAAGTT CATGGCAAGG ACATCAGTTT CCTGGTGTCT AAAAAAGCTG
    GACATATTTT GTCACTTTAC CAATGCTGCT AATAATTCAG TCCGGAGCCC ATCTATGAAG
    CTTAATGTGG TTCCTGGAAA GAACATCATG TGCCGAGATC CCGTAATAGG TGTTGGGGAG
    CCTGGAAAAG TCATCCAGAA ACTCTGCCAT TTCTCAAAAG TACCAAACAT CTCTGGAGGT
    CCTACTGGAG GGATCATAAC TTACAAATGT GTAGGCTCCC AATGGGAGGT GAAGAAAAAT
    GACTGCATCT CTGCCCCAAT AAATAGTCTG CTCCAGCTGG CCAAGGCTTT GACCAAGAGC
    CCCACTCAGG ATAAGAAGCT CCCAACATAC CTAAAGGATC TTTCTATTAG CACAGGCAAG
    GTAAAACATG AAGTCAGCTC ATTTCCTGGG AGCCTGGGAG CCATCGTTAA CATCCTTGAT
    TTGCTTTCAA CAGTTACAAC CCAAGTGAAT TCAGAGATGA TGATGAACGT TCTCTCTACG
    GTTAACACTG TCCTCAGCAA GCCCATGTTG AGTACCTGGA AGGTGTCCCA ACAGCAACAG
    ACCAACCAGA GCTCACAGCT ACTGGATTCA GTAGAAAGAT TTTCCCGAGC ATTACAGACA
    GAAGATAGCA CCTTTTTATC CATCAACCAA ACTAACGTGC AGATGAGGAG CATGGTAATA
    AAAACTGGCC ATCCAAAAAC CTACAAACAG AGCTTTGTTT TCTCAGACTT GGACCTCTGG
    GGCAATGTGG CCATTGACAA GTGCGAGGTG GGAAGCCTGC AGCAAGATTC ATCTATTGTC
    ACCGTGGCTT TCCCAACTCT CCAAACCATC CTCACTCAGG ATGTTCAAGG AAAGAATTTT
    GCCAACAGCT TAGTGATGAC AACCACTGTT AGCCACAACA TAACCATGCC ATTTAGGATT
    TCAATGACTT TTAAGAACAT CAATCCTTCA GGTGGGGTAC CACAATGTGT CTACTGGAAT
    TTCAACCTCG CCAACCACAC AGGGGGTTGG GATAGTAGTG GGTGTTATGT GCACAATGTC
    GATGGGGACA GTGTCTCCTG CATCTGTGAC CACCTAACAT CATTCTCCAT CCTCATGTCC
    CCCGACTCTC CGTACCCTGG TTCCCTCCTG GGAATACTAC TGGATATCAT TTCCTACATT
    GGGTTATGCT TTTCCATCTT GAGCTTAGCA GCCTGTCTAG TGGTGGAAGC TGTGGTGTGG
    AAATCAGTGA CCAAGAATCG GACTTCCTAT ATGCGCCACA CCTGCATAGT GAACATTGCA
    GCCTCTCTTC TGGTTGCTGA CATCTGGTTC ATTGTAGCTG CTGCCATCCA CGACCATTTT
    TACCCACTCA ATGAGACAGC CTGTGTGGCC GCCACCTTTT TCATCCACTT CTTCTACCTC
    AGCGTCTTTT TCTGGATGCT GACTCTGGGC CTCATGCTCT TCTACCGCCT GGTTTTCATC
    CTGCATGACA CAAGCAAGTC CATTCAGAAG ACTATTGCAT TTTCTCTTGG CTATGGCTGC
    CCTCTTGTCA TCTCAGTCAT CACAGTGGGG GCCACCCAAC CCCAAGAAGT CTACACCAGG
    AAGAATGCCT GCTGGCTCAA CTGGGAGGAT ACCAAGGCCC TGCTGGCCTT TGTCATCCCA
    GCACTGATCA TCGTGGTGGT GAACATGACC ATCACGGTTG TGGTCATCTC CAAGATCCTG
    AGACCCTCCA TTGGAGACAA GCCAAGCAAG CAGGAGAAGA GTAGCTTGTT TCAGATCAGC
    AAGAGCATTG GGGTCCTAAC ACCACTCTTG GGGCTCACCT GGGGTTTTGG TCTTGCCACT
    GTGTTCCAAG GAAGCAATGC TGTGTTCCAT ATTATATTTA CACTCCTCAA TGCCTTTCAG
    GGATTATTCA TTTTGCTCTT TGGATGCCTC TGGGATCAGA AGGTACAGGA AGCCTTACTA
    AAGAAGTTTT CACTGTCAAG ATGGTCTTCT CAGCACTCAA AGTCAACATC CCTAGGTTCA
    TCTACACCAG TATTTTCTAT GAGTTCTCCA ATATCAAGAA GATTTAACAA TTTATTTGGA
    AAAACAGCGG CCCCAAAGGG CCCCCCAGGC AATGATGGAA ACCTTGTTAG CAGTGGCAGC
    TCACCAATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005627501.1
    CDS399..4550
    Translation

    Target ORF information:

    RefSeq Version XM_005627444.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005627444.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAATCCC CAAGGAGAAC CACATTGTGC TTCATGCTTA TTGTGATTTA TTTTTCCCAA 
    GCTACACTGA ACTTGAATTT AGAATCTATT GTTCATCCTT TGATTCTCCA TGAACATGAA
    CTAACAGAGA AGGAGCCACT AAGGGAAAAA CGAGCAGTTG CCTCCAGCAG CCCAGTACCT
    GCAGAGTACA CTGTTGATGT GGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAGCCTATC
    AAAGCTTATG TGAACAGCCT CAGTTTCCCA ATTCCAGGGA ACAGCACAGA CCTGGCCACC
    AACATTTTAA GCATTGAGGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CGGGCTATAG GTGGCCTCAG GAGAAGTGCC TCCACAATCC CACTTGTCAA
    GAGCATGACA GCTCCCTTGC AGGGCATCGT TGCAGTTGCC TGAAAGGACT ACCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGCAGAT GTTACTTTAA GAATGTCAGT CAGACTCAAT
    GTGGGCTTTC AAGAGGACCT CAAGAATTCT TCCTCTGCTC TCTATAGGTC CTACAAGACT
    GACCTGGAAA CAGCGTTCTG GAAAGGTTAC AGTACTTTAC CAGGCTTCAA ATTGGTGACT
    GTGACAGGGT TCAGGCCCGG AAGTGTGGTT GTGACATATG AGGTGAAGAC TACAACACCA
    TCACCTCAAT TGATGCATAA AGCAAATGAG CAAGTGATGC AGAACCTGAA TCAGACCTAC
    AGAATGGACT ACAGTTCCTT TCAAGCCGTT ACTAGCAATG AAACTAAGTT CACCATCATA
    CCAGAAATCA TTTTTGAAGG GGATACGGTA AACATGATGT GTGAAACCGA CGTTTTGTCC
    TCTAACGTGT CCTGGCACCA TGTTGAAAGG CACTCTGACA TCCAGAACAG CAGCAGATTC
    TCAGTTTACA CCACCGTGCT GAACAACATG ACCTCAGTCT CCCGCCTCAC GATTTACAAC
    ATCACCCAGC GGGATGCAGG TGAATATATT TGCAAGCTGA CATTAGATAT TTTTGAATAT
    GAGTCCAGAA AAAAAATAGA TGTCACGCCC ATCCGAATTC TGGCAACTGA AGAAATGAAG
    GTGACGTGCG ACAACAATCC TATATCTTTG AACTGCTGCA GTGAGATTAA TGCTAACTGG
    AGCACAATAG AATGGAAGCA GGAGGGGAAA ATAAACATTC CAGGCAACCC TGAAACTGAC
    CTAGAGTCCA GCTGCAGCAG ATACACCCTC AAGGCAGATG GAACCCAGTG TCCGAGTGGG
    TCGGCTGGAA CCACGGGCAT CTTTGTGTGT GAGTTCATCA GCACCTACGG AGCCAGAGGC
    AGTAAGAGCA TAGAAGTGAC GTTCATCTCT GTGGGCGATA ATTCCAGCCA CAAAGGGGAA
    GTGCACGAGA TCCTCAGGCA TGATACGGAA ATGAGTACAG TTCAGAGCAC CACCGGGAAC
    AGCCTAGAAA CAAGTAAAGC CCAGGAGCAG CAAGCCAACC TAACAATAAC CCCGGACCCA
    ATTTCTGTTT CTGAGGGACA AAACTTTTCC ATAAAGTGCA TCAGCGATGT GAGTAACTAT
    GATGAGGTAT ACTGGAACAC GTCTGCTGGA ATTAAAATAT ACCAAAAGTT TTATACCACG
    AGGAGGTTTT CTGATGGAGC AGAGTCAGTG CTGACAGTGA AGACTGTGAC CCGGGAGTGG
    AACGGAACCT ATCACTGCAT ATTTAGATAC AAGAATTCAT ACAGTGTCGT AATGAAAGAT
    GTGACCGTTT ACCCACTGCC TCTAGAGCCA AACATCATGG TTGATCCTCT GGAGGCTACA
    GTTCCCTGCA AGGGTTCCCA TCACTTCAGA TGCTGCATTG ACGAGGATGA GAACTACAGA
    GTGACTTTCC AAGTGGATTC CTTATCCTTT CCTGCAGAAA AAGAAGTTAA TGGCAAGCAA
    GTGTGCTACA AATACAAGTT CATGGCAAGG ACATCAGTTT CCTGGTGTCT AAAAAAGCTG
    GACATATTTT GTCACTTTAC CAATGCTGCT AATAATTCAG TCCGGAGCCC ATCTATGAAG
    CTTAATGTGG TTCCTGGAAA GAACATCATG TGCCGAGATC CCGTAATAGG TGTTGGGGAG
    CCTGGAAAAG TCATCCAGAA ACTCTGCCAT TTCTCAAAAG TACCAAACAT CTCTGGAGGT
    CCTACTGGAG GGATCATAAC TTACAAATGT GTAGGCTCCC AATGGGAGGT GAAGAAAAAT
    GACTGCATCT CTGCCCCAAT AAATAGTCTG CTCCAGCTGG CCAAGGCTTT GACCAAGAGC
    CCCACTCAGG ATAAGAAGCT CCCAACATAC CTAAAGGATC TTTCTATTAG CACAGGCAAG
    GTAAAACATG AAGTCAGCTC ATTTCCTGGG AGCCTGGGAG CCATCGTTAA CATCCTTGAT
    TTGCTTTCAA CAGTTACAAC CCAAGTGAAT TCAGAGATGA TGATGAACGT TCTCTCTACG
    GTTAACACTG TCCTCAGCAA GCCCATGTTG AGTACCTGGA AGGTGTCCCA ACAGCAACAG
    ACCAACCAGA GCTCACAGCT ACTGGATTCA GTAGAAAGAT TTTCCCGAGC ATTACAGACA
    GAAGATAGCA CCTTTTTATC CATCAACCAA ACTAACGTGC AGATGAGGAG CATGGTAATA
    AAAACTGGCC ATCCAAAAAC CTACAAACAG AGCTTTGTTT TCTCAGACTT GGACCTCTGG
    GGCAATGTGG CCATTGACAA GTGCGAGGTG GGAAGCCTGC AGCAAGATTC ATCTATTGTC
    ACCGTGGCTT TCCCAACTCT CCAAACCATC CTCACTCAGG ATGTTCAAGG AAAGAATTTT
    GCCAACAGCT TAGTGATGAC AACCACTGTT AGCCACAACA TAACCATGCC ATTTAGGATT
    TCAATGACTT TTAAGAACAT CAATCCTTCA GGTGGGGTAC CACAATGTGT CTACTGGAAT
    TTCAACCTCG CCAACCACAC AGGGGGTTGG GATAGTAGTG GGTGTTATGT GCACAATGTC
    GATGGGGACA GTGTCTCCTG CATCTGTGAC CACCTAACAT CATTCTCCAT CCTCATGTCC
    CCCGACTCTC CGTACCCTGG TTCCCTCCTG GGAATACTAC TGGATATCAT TTCCTACATT
    GGGTTATGCT TTTCCATCTT GAGCTTAGCA GCCTGTCTAG TGGTGGAAGC TGTGGTGTGG
    AAATCAGTGA CCAAGAATCG GACTTCCTAT ATGCGCCACA CCTGCATAGT GAACATTGCA
    GCCTCTCTTC TGGTTGCTGA CATCTGGTTC ATTGTAGCTG CTGCCATCCA CGACCATTTT
    TACCCACTCA ATGAGACAGC CTGTGTGGCC GCCACCTTTT TCATCCACTT CTTCTACCTC
    AGCGTCTTTT TCTGGATGCT GACTCTGGGC CTCATGCTCT TCTACCGCCT GGTTTTCATC
    CTGCATGACA CAAGCAAGTC CATTCAGAAG ACTATTGCAT TTTCTCTTGG CTATGGCTGC
    CCTCTTGTCA TCTCAGTCAT CACAGTGGGG GCCACCCAAC CCCAAGAAGT CTACACCAGG
    AAGAATGCCT GCTGGCTCAA CTGGGAGGAT ACCAAGGCCC TGCTGGCCTT TGTCATCCCA
    GCACTGATCA TCGTGGTGGT GAACATGACC ATCACGGTTG TGGTCATCTC CAAGATCCTG
    AGACCCTCCA TTGGAGACAA GCCAAGCAAG CAGGAGAAGA GTAGCTTGTT TCAGATCAGC
    AAGAGCATTG GGGTCCTAAC ACCACTCTTG GGGCTCACCT GGGGTTTTGG TCTTGCCACT
    GTGTTCCAAG GAAGCAATGC TGTGTTCCAT ATTATATTTA CACTCCTCAA TGCCTTTCAG
    GGATTATTCA TTTTGCTCTT TGGATGCCTC TGGGATCAGA AGGTACAGGA AGCCTTACTA
    AAGAAGTTTT CACTGTCAAG ATGGTCTTCT CAGCACTCAA AGTCAACATC CCTAGGTTCA
    TCTACACCAG TATTTTCTAT GAGTTCTCCA ATATCAAGAA GATTTAACAA TTTATTTGGA
    AAAACAGCGG CCCCAAAGGG CCCCCCAGGC AATGATGGAA ACCTTGTTAG CAGTGGCAGC
    TCACCAATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe15373
    Clone ID Related Accession (Same CDS sequence) XM_005627444.2 , XM_005627444.3
    Accession Version XM_005627444.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4152bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product adhesion G protein-coupled receptor F5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005627444.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAATCCC CAAGGAGAAC CACATTGTGC TTCATGCTTA TTGTGATTTA TTTTTCCCAA 
    GCTACACTGA ACTTGAATTT AGAATCTATT GTTCATCCTT TGATTCTCCA TGAACATGAA
    CTAACAGAGA AGGAGCCACT AAGGGAAAAA CGAGCAGTTG CCTCCAGCAG CCCAGTACCT
    GCAGAGTACA CTGTTGATGT GGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAGCCTATC
    AAAGCTTATG TGAACAGCCT CAGTTTCCCA ATTCCAGGGA ACAGCACAGA CCTGGCCACC
    AACATTTTAA GCATTGAGGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CGGGCTATAG GTGGCCTCAG GAGAAGTGCC TCCACAATCC CACTTGTCAA
    GAGCATGACA GCTCCCTTGC AGGGCATCGT TGCAGTTGCC TGAAAGGACT ACCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGCAGAT GTTACTTTAA GAATGTCAGT CAGACTCAAT
    GTGGGCTTTC AAGAGGACCT CAAGAATTCT TCCTCTGCTC TCTATAGGTC CTACAAGACT
    GACCTGGAAA CAGCGTTCTG GAAAGGTTAC AGTACTTTAC CAGGCTTCAA ATTGGTGACT
    GTGACAGGGT TCAGGCCCGG AAGTGTGGTT GTGACATATG AGGTGAAGAC TACAACACCA
    TCACCTCAAT TGATGCATAA AGCAAATGAG CAAGTGATGC AGAACCTGAA TCAGACCTAC
    AGAATGGACT ACAGTTCCTT TCAAGCCGTT ACTAGCAATG AAACTAAGTT CACCATCATA
    CCAGAAATCA TTTTTGAAGG GGATACGGTA AACATGATGT GTGAAACCGA CGTTTTGTCC
    TCTAACGTGT CCTGGCACCA TGTTGAAAGG CACTCTGACA TCCAGAACAG CAGCAGATTC
    TCAGTTTACA CCACCGTGCT GAACAACATG ACCTCAGTCT CCCGCCTCAC GATTTACAAC
    ATCACCCAGC GGGATGCAGG TGAATATATT TGCAAGCTGA CATTAGATAT TTTTGAATAT
    GAGTCCAGAA AAAAAATAGA TGTCACGCCC ATCCGAATTC TGGCAACTGA AGAAATGAAG
    GTGACGTGCG ACAACAATCC TATATCTTTG AACTGCTGCA GTGAGATTAA TGCTAACTGG
    AGCACAATAG AATGGAAGCA GGAGGGGAAA ATAAACATTC CAGGCAACCC TGAAACTGAC
    CTAGAGTCCA GCTGCAGCAG ATACACCCTC AAGGCAGATG GAACCCAGTG TCCGAGTGGG
    TCGGCTGGAA CCACGGGCAT CTTTGTGTGT GAGTTCATCA GCACCTACGG AGCCAGAGGC
    AGTAAGAGCA TAGAAGTGAC GTTCATCTCT GTGGGCGATA ATTCCAGCCA CAAAGGGGAA
    GTGCACGAGA TCCTCAGGCA TGATACGGAA ATGAGTACAG TTCAGAGCAC CACCGGGAAC
    AGCCTAGAAA CAAGTAAAGC CCAGGAGCAG CAAGCCAACC TAACAATAAC CCCGGACCCA
    ATTTCTGTTT CTGAGGGACA AAACTTTTCC ATAAAGTGCA TCAGCGATGT GAGTAACTAT
    GATGAGGTAT ACTGGAACAC GTCTGCTGGA ATTAAAATAT ACCAAAAGTT TTATACCACG
    AGGAGGTTTT CTGATGGAGC AGAGTCAGTG CTGACAGTGA AGACTGTGAC CCGGGAGTGG
    AACGGAACCT ATCACTGCAT ATTTAGATAC AAGAATTCAT ACAGTGTCGT AATGAAAGAT
    GTGACCGTTT ACCCACTGCC TCTAGAGCCA AACATCATGG TTGATCCTCT GGAGGCTACA
    GTTCCCTGCA AGGGTTCCCA TCACTTCAGA TGCTGCATTG ACGAGGATGA GAACTACAGA
    GTGACTTTCC AAGTGGATTC CTTATCCTTT CCTGCAGAAA AAGAAGTTAA TGGCAAGCAA
    GTGTGCTACA AATACAAGTT CATGGCAAGG ACATCAGTTT CCTGGTGTCT AAAAAAGCTG
    GACATATTTT GTCACTTTAC CAATGCTGCT AATAATTCAG TCCGGAGCCC ATCTATGAAG
    CTTAATGTGG TTCCTGGAAA GAACATCATG TGCCGAGATC CCGTAATAGG TGTTGGGGAG
    CCTGGAAAAG TCATCCAGAA ACTCTGCCAT TTCTCAAAAG TACCAAACAT CTCTGGAGGT
    CCTACTGGAG GGATCATAAC TTACAAATGT GTAGGCTCCC AATGGGAGGT GAAGAAAAAT
    GACTGCATCT CTGCCCCAAT AAATAGTCTG CTCCAGCTGG CCAAGGCTTT GACCAAGAGC
    CCCACTCAGG ATAAGAAGCT CCCAACATAC CTAAAGGATC TTTCTATTAG CACAGGCAAG
    GTAAAACATG AAGTCAGCTC ATTTCCTGGG AGCCTGGGAG CCATCGTTAA CATCCTTGAT
    TTGCTTTCAA CAGTTACAAC CCAAGTGAAT TCAGAGATGA TGATGAACGT TCTCTCTACG
    GTTAACACTG TCCTCAGCAA GCCCATGTTG AGTACCTGGA AGGTGTCCCA ACAGCAACAG
    ACCAACCAGA GCTCACAGCT ACTGGATTCA GTAGAAAGAT TTTCCCGAGC ATTACAGACA
    GAAGATAGCA CCTTTTTATC CATCAACCAA ACTAACGTGC AGATGAGGAG CATGGTAATA
    AAAACTGGCC ATCCAAAAAC CTACAAACAG AGCTTTGTTT TCTCAGACTT GGACCTCTGG
    GGCAATGTGG CCATTGACAA GTGCGAGGTG GGAAGCCTGC AGCAAGATTC ATCTATTGTC
    ACCGTGGCTT TCCCAACTCT CCAAACCATC CTCACTCAGG ATGTTCAAGG AAAGAATTTT
    GCCAACAGCT TAGTGATGAC AACCACTGTT AGCCACAACA TAACCATGCC ATTTAGGATT
    TCAATGACTT TTAAGAACAT CAATCCTTCA GGTGGGGTAC CACAATGTGT CTACTGGAAT
    TTCAACCTCG CCAACCACAC AGGGGGTTGG GATAGTAGTG GGTGTTATGT GCACAATGTC
    GATGGGGACA GTGTCTCCTG CATCTGTGAC CACCTAACAT CATTCTCCAT CCTCATGTCC
    CCCGACTCTC CGTACCCTGG TTCCCTCCTG GGAATACTAC TGGATATCAT TTCCTACATT
    GGGTTATGCT TTTCCATCTT GAGCTTAGCA GCCTGTCTAG TGGTGGAAGC TGTGGTGTGG
    AAATCAGTGA CCAAGAATCG GACTTCCTAT ATGCGCCACA CCTGCATAGT GAACATTGCA
    GCCTCTCTTC TGGTTGCTGA CATCTGGTTC ATTGTAGCTG CTGCCATCCA CGACCATTTT
    TACCCACTCA ATGAGACAGC CTGTGTGGCC GCCACCTTTT TCATCCACTT CTTCTACCTC
    AGCGTCTTTT TCTGGATGCT GACTCTGGGC CTCATGCTCT TCTACCGCCT GGTTTTCATC
    CTGCATGACA CAAGCAAGTC CATTCAGAAG ACTATTGCAT TTTCTCTTGG CTATGGCTGC
    CCTCTTGTCA TCTCAGTCAT CACAGTGGGG GCCACCCAAC CCCAAGAAGT CTACACCAGG
    AAGAATGCCT GCTGGCTCAA CTGGGAGGAT ACCAAGGCCC TGCTGGCCTT TGTCATCCCA
    GCACTGATCA TCGTGGTGGT GAACATGACC ATCACGGTTG TGGTCATCTC CAAGATCCTG
    AGACCCTCCA TTGGAGACAA GCCAAGCAAG CAGGAGAAGA GTAGCTTGTT TCAGATCAGC
    AAGAGCATTG GGGTCCTAAC ACCACTCTTG GGGCTCACCT GGGGTTTTGG TCTTGCCACT
    GTGTTCCAAG GAAGCAATGC TGTGTTCCAT ATTATATTTA CACTCCTCAA TGCCTTTCAG
    GGATTATTCA TTTTGCTCTT TGGATGCCTC TGGGATCAGA AGGTACAGGA AGCCTTACTA
    AAGAAGTTTT CACTGTCAAG ATGGTCTTCT CAGCACTCAA AGTCAACATC CCTAGGTTCA
    TCTACACCAG TATTTTCTAT GAGTTCTCCA ATATCAAGAA GATTTAACAA TTTATTTGGA
    AAAACAGCGG CCCCAAAGGG CCCCCCAGGC AATGATGGAA ACCTTGTTAG CAGTGGCAGC
    TCACCAATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005627501.1
    CDS419..4570
    Translation

    Target ORF information:

    RefSeq Version XM_005627444.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005627444.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAATCCC CAAGGAGAAC CACATTGTGC TTCATGCTTA TTGTGATTTA TTTTTCCCAA 
    GCTACACTGA ACTTGAATTT AGAATCTATT GTTCATCCTT TGATTCTCCA TGAACATGAA
    CTAACAGAGA AGGAGCCACT AAGGGAAAAA CGAGCAGTTG CCTCCAGCAG CCCAGTACCT
    GCAGAGTACA CTGTTGATGT GGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAGCCTATC
    AAAGCTTATG TGAACAGCCT CAGTTTCCCA ATTCCAGGGA ACAGCACAGA CCTGGCCACC
    AACATTTTAA GCATTGAGGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CGGGCTATAG GTGGCCTCAG GAGAAGTGCC TCCACAATCC CACTTGTCAA
    GAGCATGACA GCTCCCTTGC AGGGCATCGT TGCAGTTGCC TGAAAGGACT ACCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGCAGAT GTTACTTTAA GAATGTCAGT CAGACTCAAT
    GTGGGCTTTC AAGAGGACCT CAAGAATTCT TCCTCTGCTC TCTATAGGTC CTACAAGACT
    GACCTGGAAA CAGCGTTCTG GAAAGGTTAC AGTACTTTAC CAGGCTTCAA ATTGGTGACT
    GTGACAGGGT TCAGGCCCGG AAGTGTGGTT GTGACATATG AGGTGAAGAC TACAACACCA
    TCACCTCAAT TGATGCATAA AGCAAATGAG CAAGTGATGC AGAACCTGAA TCAGACCTAC
    AGAATGGACT ACAGTTCCTT TCAAGCCGTT ACTAGCAATG AAACTAAGTT CACCATCATA
    CCAGAAATCA TTTTTGAAGG GGATACGGTA AACATGATGT GTGAAACCGA CGTTTTGTCC
    TCTAACGTGT CCTGGCACCA TGTTGAAAGG CACTCTGACA TCCAGAACAG CAGCAGATTC
    TCAGTTTACA CCACCGTGCT GAACAACATG ACCTCAGTCT CCCGCCTCAC GATTTACAAC
    ATCACCCAGC GGGATGCAGG TGAATATATT TGCAAGCTGA CATTAGATAT TTTTGAATAT
    GAGTCCAGAA AAAAAATAGA TGTCACGCCC ATCCGAATTC TGGCAACTGA AGAAATGAAG
    GTGACGTGCG ACAACAATCC TATATCTTTG AACTGCTGCA GTGAGATTAA TGCTAACTGG
    AGCACAATAG AATGGAAGCA GGAGGGGAAA ATAAACATTC CAGGCAACCC TGAAACTGAC
    CTAGAGTCCA GCTGCAGCAG ATACACCCTC AAGGCAGATG GAACCCAGTG TCCGAGTGGG
    TCGGCTGGAA CCACGGGCAT CTTTGTGTGT GAGTTCATCA GCACCTACGG AGCCAGAGGC
    AGTAAGAGCA TAGAAGTGAC GTTCATCTCT GTGGGCGATA ATTCCAGCCA CAAAGGGGAA
    GTGCACGAGA TCCTCAGGCA TGATACGGAA ATGAGTACAG TTCAGAGCAC CACCGGGAAC
    AGCCTAGAAA CAAGTAAAGC CCAGGAGCAG CAAGCCAACC TAACAATAAC CCCGGACCCA
    ATTTCTGTTT CTGAGGGACA AAACTTTTCC ATAAAGTGCA TCAGCGATGT GAGTAACTAT
    GATGAGGTAT ACTGGAACAC GTCTGCTGGA ATTAAAATAT ACCAAAAGTT TTATACCACG
    AGGAGGTTTT CTGATGGAGC AGAGTCAGTG CTGACAGTGA AGACTGTGAC CCGGGAGTGG
    AACGGAACCT ATCACTGCAT ATTTAGATAC AAGAATTCAT ACAGTGTCGT AATGAAAGAT
    GTGACCGTTT ACCCACTGCC TCTAGAGCCA AACATCATGG TTGATCCTCT GGAGGCTACA
    GTTCCCTGCA AGGGTTCCCA TCACTTCAGA TGCTGCATTG ACGAGGATGA GAACTACAGA
    GTGACTTTCC AAGTGGATTC CTTATCCTTT CCTGCAGAAA AAGAAGTTAA TGGCAAGCAA
    GTGTGCTACA AATACAAGTT CATGGCAAGG ACATCAGTTT CCTGGTGTCT AAAAAAGCTG
    GACATATTTT GTCACTTTAC CAATGCTGCT AATAATTCAG TCCGGAGCCC ATCTATGAAG
    CTTAATGTGG TTCCTGGAAA GAACATCATG TGCCGAGATC CCGTAATAGG TGTTGGGGAG
    CCTGGAAAAG TCATCCAGAA ACTCTGCCAT TTCTCAAAAG TACCAAACAT CTCTGGAGGT
    CCTACTGGAG GGATCATAAC TTACAAATGT GTAGGCTCCC AATGGGAGGT GAAGAAAAAT
    GACTGCATCT CTGCCCCAAT AAATAGTCTG CTCCAGCTGG CCAAGGCTTT GACCAAGAGC
    CCCACTCAGG ATAAGAAGCT CCCAACATAC CTAAAGGATC TTTCTATTAG CACAGGCAAG
    GTAAAACATG AAGTCAGCTC ATTTCCTGGG AGCCTGGGAG CCATCGTTAA CATCCTTGAT
    TTGCTTTCAA CAGTTACAAC CCAAGTGAAT TCAGAGATGA TGATGAACGT TCTCTCTACG
    GTTAACACTG TCCTCAGCAA GCCCATGTTG AGTACCTGGA AGGTGTCCCA ACAGCAACAG
    ACCAACCAGA GCTCACAGCT ACTGGATTCA GTAGAAAGAT TTTCCCGAGC ATTACAGACA
    GAAGATAGCA CCTTTTTATC CATCAACCAA ACTAACGTGC AGATGAGGAG CATGGTAATA
    AAAACTGGCC ATCCAAAAAC CTACAAACAG AGCTTTGTTT TCTCAGACTT GGACCTCTGG
    GGCAATGTGG CCATTGACAA GTGCGAGGTG GGAAGCCTGC AGCAAGATTC ATCTATTGTC
    ACCGTGGCTT TCCCAACTCT CCAAACCATC CTCACTCAGG ATGTTCAAGG AAAGAATTTT
    GCCAACAGCT TAGTGATGAC AACCACTGTT AGCCACAACA TAACCATGCC ATTTAGGATT
    TCAATGACTT TTAAGAACAT CAATCCTTCA GGTGGGGTAC CACAATGTGT CTACTGGAAT
    TTCAACCTCG CCAACCACAC AGGGGGTTGG GATAGTAGTG GGTGTTATGT GCACAATGTC
    GATGGGGACA GTGTCTCCTG CATCTGTGAC CACCTAACAT CATTCTCCAT CCTCATGTCC
    CCCGACTCTC CGTACCCTGG TTCCCTCCTG GGAATACTAC TGGATATCAT TTCCTACATT
    GGGTTATGCT TTTCCATCTT GAGCTTAGCA GCCTGTCTAG TGGTGGAAGC TGTGGTGTGG
    AAATCAGTGA CCAAGAATCG GACTTCCTAT ATGCGCCACA CCTGCATAGT GAACATTGCA
    GCCTCTCTTC TGGTTGCTGA CATCTGGTTC ATTGTAGCTG CTGCCATCCA CGACCATTTT
    TACCCACTCA ATGAGACAGC CTGTGTGGCC GCCACCTTTT TCATCCACTT CTTCTACCTC
    AGCGTCTTTT TCTGGATGCT GACTCTGGGC CTCATGCTCT TCTACCGCCT GGTTTTCATC
    CTGCATGACA CAAGCAAGTC CATTCAGAAG ACTATTGCAT TTTCTCTTGG CTATGGCTGC
    CCTCTTGTCA TCTCAGTCAT CACAGTGGGG GCCACCCAAC CCCAAGAAGT CTACACCAGG
    AAGAATGCCT GCTGGCTCAA CTGGGAGGAT ACCAAGGCCC TGCTGGCCTT TGTCATCCCA
    GCACTGATCA TCGTGGTGGT GAACATGACC ATCACGGTTG TGGTCATCTC CAAGATCCTG
    AGACCCTCCA TTGGAGACAA GCCAAGCAAG CAGGAGAAGA GTAGCTTGTT TCAGATCAGC
    AAGAGCATTG GGGTCCTAAC ACCACTCTTG GGGCTCACCT GGGGTTTTGG TCTTGCCACT
    GTGTTCCAAG GAAGCAATGC TGTGTTCCAT ATTATATTTA CACTCCTCAA TGCCTTTCAG
    GGATTATTCA TTTTGCTCTT TGGATGCCTC TGGGATCAGA AGGTACAGGA AGCCTTACTA
    AAGAAGTTTT CACTGTCAAG ATGGTCTTCT CAGCACTCAA AGTCAACATC CCTAGGTTCA
    TCTACACCAG TATTTTCTAT GAGTTCTCCA ATATCAAGAA GATTTAACAA TTTATTTGGA
    AAAACAGCGG CCCCAAAGGG CCCCCCAGGC AATGATGGAA ACCTTGTTAG CAGTGGCAGC
    TCACCAATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe15374
    Clone ID Related Accession (Same CDS sequence) XM_005627445.3 , XM_005627445.2
    Accession Version XM_005627445.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4047bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product adhesion G protein-coupled receptor F5 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005627445.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAAATCCC CAAGGAGAAC CACATTGTGC TTCATGCTTA TTGTGATTTA TTTTTCCCAA 
    GCTACACTGA ACTTGAATTT AGAATCTATT GTTCATCCTT TGATTCTCCA TGAACATGAA
    CTAACAGAGA AGGAGCCACT AAGGGAAAAA CGAGCAGTTG CCTCCAGCAG CCCAGTACCT
    GCAGAGTACA CTGTTGATGT GGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAGCCTATC
    AAAGCTTATG TGAACAGCCT CAGTTTCCCA ATTCCAGGGA ACAGCACAGA CCTGGCCACC
    AACATTTTAA GCATTGAGGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CGGGCTATAG GTGGCCTCAG GAGAAGTGCC TCCACAATCC CACTTGTCAA
    GAGCATGACA GCTCCCTTGC AGGGCATCGT TGCAGTTGCC TGAAAGGACT ACCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGCAGAT GTTACTTTAA GAATGTCAGT CAGACTCAAT
    GTGGGCTTTC AAGAGGACCT CAAGAATTCT TCCTCTGCTC TCTATAGGTC CTACAAGACT
    GACCTGGAAA CAGCGTTCTG GAAAGGTTAC AGTACTTTAC CAGGCTTCAA ATTGGTGACT
    GTGACAGGGT TCAGGCCCGG AAGTGTGGTT GTGACATATG AGGTGAAGAC TACAACACCA
    TCACCTCAAT TGATGCATAA AGCAAATGAG CAAGTGATGC AGAACCTGAA TCAGACCTAC
    AGAATGGACT ACAGTTCCTT TCAAGCCGTT ACTAGCAATG AAACTAAGTT CACCATCATA
    CCAGAAATCA TTTTTGAAGG GGATACGGTA AACATGATGT GTGAAACCGA CGTTTTGTCC
    TCTAACGTGT CCTGGCACCA TGTTGAAAGG CACTCTGACA TCCAGAACAG CAGCAGATTC
    TCAGTTTACA CCACCGTGCT GAACAACATG ACCTCAGTCT CCCGCCTCAC GATTTACAAC
    ATCACCCAGC GGGATGCAGG TGAATATATT TGCAAGCTGA CATTAGATAT TTTTGAATAT
    GAGTCCAGAA AAAAAATAGA TGTCACGCCC ATCCGAATTC TGGCAACTGA AGAAATGAAG
    GTGACGTGCG ACAACAATCC TATATCTTTG AACTGCTGCA GTGAGATTAA TGCTAACTGG
    AGCACAATAG AATGGAAGCA GGAGGGGAAA ATAAACATTC CAGGCAACCC TGAAACTGAC
    CTAGAGTCCA GCTGCAGCAG ATACACCCTC AAGGCAGATG GAACCCAGTG TCCGAGTGGG
    TCGGCTGGAA CCACGGGCAT CTTTGTGTGT GAGTTCATCA GCACCTACGG AGCCAGAGGC
    AGTAAGAGCA TAGAAGTGAC GTTCATCTCT GTGGCCAACC TAACAATAAC CCCGGACCCA
    ATTTCTGTTT CTGAGGGACA AAACTTTTCC ATAAAGTGCA TCAGCGATGT GAGTAACTAT
    GATGAGGTAT ACTGGAACAC GTCTGCTGGA ATTAAAATAT ACCAAAAGTT TTATACCACG
    AGGAGGTTTT CTGATGGAGC AGAGTCAGTG CTGACAGTGA AGACTGTGAC CCGGGAGTGG
    AACGGAACCT ATCACTGCAT ATTTAGATAC AAGAATTCAT ACAGTGTCGT AATGAAAGAT
    GTGACCGTTT ACCCACTGCC TCTAGAGCCA AACATCATGG TTGATCCTCT GGAGGCTACA
    GTTCCCTGCA AGGGTTCCCA TCACTTCAGA TGCTGCATTG ACGAGGATGA GAACTACAGA
    GTGACTTTCC AAGTGGATTC CTTATCCTTT CCTGCAGAAA AAGAAGTTAA TGGCAAGCAA
    GTGTGCTACA AATACAAGTT CATGGCAAGG ACATCAGTTT CCTGGTGTCT AAAAAAGCTG
    GACATATTTT GTCACTTTAC CAATGCTGCT AATAATTCAG TCCGGAGCCC ATCTATGAAG
    CTTAATGTGG TTCCTGGAAA GAACATCATG TGCCGAGATC CCGTAATAGG TGTTGGGGAG
    CCTGGAAAAG TCATCCAGAA ACTCTGCCAT TTCTCAAAAG TACCAAACAT CTCTGGAGGT
    CCTACTGGAG GGATCATAAC TTACAAATGT GTAGGCTCCC AATGGGAGGT GAAGAAAAAT
    GACTGCATCT CTGCCCCAAT AAATAGTCTG CTCCAGCTGG CCAAGGCTTT GACCAAGAGC
    CCCACTCAGG ATAAGAAGCT CCCAACATAC CTAAAGGATC TTTCTATTAG CACAGGCAAG
    GTAAAACATG AAGTCAGCTC ATTTCCTGGG AGCCTGGGAG CCATCGTTAA CATCCTTGAT
    TTGCTTTCAA CAGTTACAAC CCAAGTGAAT TCAGAGATGA TGATGAACGT TCTCTCTACG
    GTTAACACTG TCCTCAGCAA GCCCATGTTG AGTACCTGGA AGGTGTCCCA ACAGCAACAG
    ACCAACCAGA GCTCACAGCT ACTGGATTCA GTAGAAAGAT TTTCCCGAGC ATTACAGACA
    GAAGATAGCA CCTTTTTATC CATCAACCAA ACTAACGTGC AGATGAGGAG CATGGTAATA
    AAAACTGGCC ATCCAAAAAC CTACAAACAG AGCTTTGTTT TCTCAGACTT GGACCTCTGG
    GGCAATGTGG CCATTGACAA GTGCGAGGTG GGAAGCCTGC AGCAAGATTC ATCTATTGTC
    ACCGTGGCTT TCCCAACTCT CCAAACCATC CTCACTCAGG ATGTTCAAGG AAAGAATTTT
    GCCAACAGCT TAGTGATGAC AACCACTGTT AGCCACAACA TAACCATGCC ATTTAGGATT
    TCAATGACTT TTAAGAACAT CAATCCTTCA GGTGGGGTAC CACAATGTGT CTACTGGAAT
    TTCAACCTCG CCAACCACAC AGGGGGTTGG GATAGTAGTG GGTGTTATGT GCACAATGTC
    GATGGGGACA GTGTCTCCTG CATCTGTGAC CACCTAACAT CATTCTCCAT CCTCATGTCC
    CCCGACTCTC CGTACCCTGG TTCCCTCCTG GGAATACTAC TGGATATCAT TTCCTACATT
    GGGTTATGCT TTTCCATCTT GAGCTTAGCA GCCTGTCTAG TGGTGGAAGC TGTGGTGTGG
    AAATCAGTGA CCAAGAATCG GACTTCCTAT ATGCGCCACA CCTGCATAGT GAACATTGCA
    GCCTCTCTTC TGGTTGCTGA CATCTGGTTC ATTGTAGCTG CTGCCATCCA CGACCATTTT
    TACCCACTCA ATGAGACAGC CTGTGTGGCC GCCACCTTTT TCATCCACTT CTTCTACCTC
    AGCGTCTTTT TCTGGATGCT GACTCTGGGC CTCATGCTCT TCTACCGCCT GGTTTTCATC
    CTGCATGACA CAAGCAAGTC CATTCAGAAG ACTATTGCAT TTTCTCTTGG CTATGGCTGC
    CCTCTTGTCA TCTCAGTCAT CACAGTGGGG GCCACCCAAC CCCAAGAAGT CTACACCAGG
    AAGAATGCCT GCTGGCTCAA CTGGGAGGAT ACCAAGGCCC TGCTGGCCTT TGTCATCCCA
    GCACTGATCA TCGTGGTGGT GAACATGACC ATCACGGTTG TGGTCATCTC CAAGATCCTG
    AGACCCTCCA TTGGAGACAA GCCAAGCAAG CAGGAGAAGA GTAGCTTGTT TCAGATCAGC
    AAGAGCATTG GGGTCCTAAC ACCACTCTTG GGGCTCACCT GGGGTTTTGG TCTTGCCACT
    GTGTTCCAAG GAAGCAATGC TGTGTTCCAT ATTATATTTA CACTCCTCAA TGCCTTTCAG
    GGATTATTCA TTTTGCTCTT TGGATGCCTC TGGGATCAGA AGGTACAGGA AGCCTTACTA
    AAGAAGTTTT CACTGTCAAG ATGGTCTTCT CAGCACTCAA AGTCAACATC CCTAGGTTCA
    TCTACACCAG TATTTTCTAT GAGTTCTCCA ATATCAAGAA GATTTAACAA TTTATTTGGA
    AAAACAGGAA CGTACAATGT TTCCACCCCA GAAACAACCA GCTCATCCCT GGAAAACACA
    TCCAGTGCTT ACTCCTTGCT GAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005627502.1
    CDS410..4456
    Translation

    Target ORF information:

    RefSeq Version XM_005627445.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005627445.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAAATCCC CAAGGAGAAC CACATTGTGC TTCATGCTTA TTGTGATTTA TTTTTCCCAA 
    GCTACACTGA ACTTGAATTT AGAATCTATT GTTCATCCTT TGATTCTCCA TGAACATGAA
    CTAACAGAGA AGGAGCCACT AAGGGAAAAA CGAGCAGTTG CCTCCAGCAG CCCAGTACCT
    GCAGAGTACA CTGTTGATGT GGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAGCCTATC
    AAAGCTTATG TGAACAGCCT CAGTTTCCCA ATTCCAGGGA ACAGCACAGA CCTGGCCACC
    AACATTTTAA GCATTGAGGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CGGGCTATAG GTGGCCTCAG GAGAAGTGCC TCCACAATCC CACTTGTCAA
    GAGCATGACA GCTCCCTTGC AGGGCATCGT TGCAGTTGCC TGAAAGGACT ACCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGCAGAT GTTACTTTAA GAATGTCAGT CAGACTCAAT
    GTGGGCTTTC AAGAGGACCT CAAGAATTCT TCCTCTGCTC TCTATAGGTC CTACAAGACT
    GACCTGGAAA CAGCGTTCTG GAAAGGTTAC AGTACTTTAC CAGGCTTCAA ATTGGTGACT
    GTGACAGGGT TCAGGCCCGG AAGTGTGGTT GTGACATATG AGGTGAAGAC TACAACACCA
    TCACCTCAAT TGATGCATAA AGCAAATGAG CAAGTGATGC AGAACCTGAA TCAGACCTAC
    AGAATGGACT ACAGTTCCTT TCAAGCCGTT ACTAGCAATG AAACTAAGTT CACCATCATA
    CCAGAAATCA TTTTTGAAGG GGATACGGTA AACATGATGT GTGAAACCGA CGTTTTGTCC
    TCTAACGTGT CCTGGCACCA TGTTGAAAGG CACTCTGACA TCCAGAACAG CAGCAGATTC
    TCAGTTTACA CCACCGTGCT GAACAACATG ACCTCAGTCT CCCGCCTCAC GATTTACAAC
    ATCACCCAGC GGGATGCAGG TGAATATATT TGCAAGCTGA CATTAGATAT TTTTGAATAT
    GAGTCCAGAA AAAAAATAGA TGTCACGCCC ATCCGAATTC TGGCAACTGA AGAAATGAAG
    GTGACGTGCG ACAACAATCC TATATCTTTG AACTGCTGCA GTGAGATTAA TGCTAACTGG
    AGCACAATAG AATGGAAGCA GGAGGGGAAA ATAAACATTC CAGGCAACCC TGAAACTGAC
    CTAGAGTCCA GCTGCAGCAG ATACACCCTC AAGGCAGATG GAACCCAGTG TCCGAGTGGG
    TCGGCTGGAA CCACGGGCAT CTTTGTGTGT GAGTTCATCA GCACCTACGG AGCCAGAGGC
    AGTAAGAGCA TAGAAGTGAC GTTCATCTCT GTGGCCAACC TAACAATAAC CCCGGACCCA
    ATTTCTGTTT CTGAGGGACA AAACTTTTCC ATAAAGTGCA TCAGCGATGT GAGTAACTAT
    GATGAGGTAT ACTGGAACAC GTCTGCTGGA ATTAAAATAT ACCAAAAGTT TTATACCACG
    AGGAGGTTTT CTGATGGAGC AGAGTCAGTG CTGACAGTGA AGACTGTGAC CCGGGAGTGG
    AACGGAACCT ATCACTGCAT ATTTAGATAC AAGAATTCAT ACAGTGTCGT AATGAAAGAT
    GTGACCGTTT ACCCACTGCC TCTAGAGCCA AACATCATGG TTGATCCTCT GGAGGCTACA
    GTTCCCTGCA AGGGTTCCCA TCACTTCAGA TGCTGCATTG ACGAGGATGA GAACTACAGA
    GTGACTTTCC AAGTGGATTC CTTATCCTTT CCTGCAGAAA AAGAAGTTAA TGGCAAGCAA
    GTGTGCTACA AATACAAGTT CATGGCAAGG ACATCAGTTT CCTGGTGTCT AAAAAAGCTG
    GACATATTTT GTCACTTTAC CAATGCTGCT AATAATTCAG TCCGGAGCCC ATCTATGAAG
    CTTAATGTGG TTCCTGGAAA GAACATCATG TGCCGAGATC CCGTAATAGG TGTTGGGGAG
    CCTGGAAAAG TCATCCAGAA ACTCTGCCAT TTCTCAAAAG TACCAAACAT CTCTGGAGGT
    CCTACTGGAG GGATCATAAC TTACAAATGT GTAGGCTCCC AATGGGAGGT GAAGAAAAAT
    GACTGCATCT CTGCCCCAAT AAATAGTCTG CTCCAGCTGG CCAAGGCTTT GACCAAGAGC
    CCCACTCAGG ATAAGAAGCT CCCAACATAC CTAAAGGATC TTTCTATTAG CACAGGCAAG
    GTAAAACATG AAGTCAGCTC ATTTCCTGGG AGCCTGGGAG CCATCGTTAA CATCCTTGAT
    TTGCTTTCAA CAGTTACAAC CCAAGTGAAT TCAGAGATGA TGATGAACGT TCTCTCTACG
    GTTAACACTG TCCTCAGCAA GCCCATGTTG AGTACCTGGA AGGTGTCCCA ACAGCAACAG
    ACCAACCAGA GCTCACAGCT ACTGGATTCA GTAGAAAGAT TTTCCCGAGC ATTACAGACA
    GAAGATAGCA CCTTTTTATC CATCAACCAA ACTAACGTGC AGATGAGGAG CATGGTAATA
    AAAACTGGCC ATCCAAAAAC CTACAAACAG AGCTTTGTTT TCTCAGACTT GGACCTCTGG
    GGCAATGTGG CCATTGACAA GTGCGAGGTG GGAAGCCTGC AGCAAGATTC ATCTATTGTC
    ACCGTGGCTT TCCCAACTCT CCAAACCATC CTCACTCAGG ATGTTCAAGG AAAGAATTTT
    GCCAACAGCT TAGTGATGAC AACCACTGTT AGCCACAACA TAACCATGCC ATTTAGGATT
    TCAATGACTT TTAAGAACAT CAATCCTTCA GGTGGGGTAC CACAATGTGT CTACTGGAAT
    TTCAACCTCG CCAACCACAC AGGGGGTTGG GATAGTAGTG GGTGTTATGT GCACAATGTC
    GATGGGGACA GTGTCTCCTG CATCTGTGAC CACCTAACAT CATTCTCCAT CCTCATGTCC
    CCCGACTCTC CGTACCCTGG TTCCCTCCTG GGAATACTAC TGGATATCAT TTCCTACATT
    GGGTTATGCT TTTCCATCTT GAGCTTAGCA GCCTGTCTAG TGGTGGAAGC TGTGGTGTGG
    AAATCAGTGA CCAAGAATCG GACTTCCTAT ATGCGCCACA CCTGCATAGT GAACATTGCA
    GCCTCTCTTC TGGTTGCTGA CATCTGGTTC ATTGTAGCTG CTGCCATCCA CGACCATTTT
    TACCCACTCA ATGAGACAGC CTGTGTGGCC GCCACCTTTT TCATCCACTT CTTCTACCTC
    AGCGTCTTTT TCTGGATGCT GACTCTGGGC CTCATGCTCT TCTACCGCCT GGTTTTCATC
    CTGCATGACA CAAGCAAGTC CATTCAGAAG ACTATTGCAT TTTCTCTTGG CTATGGCTGC
    CCTCTTGTCA TCTCAGTCAT CACAGTGGGG GCCACCCAAC CCCAAGAAGT CTACACCAGG
    AAGAATGCCT GCTGGCTCAA CTGGGAGGAT ACCAAGGCCC TGCTGGCCTT TGTCATCCCA
    GCACTGATCA TCGTGGTGGT GAACATGACC ATCACGGTTG TGGTCATCTC CAAGATCCTG
    AGACCCTCCA TTGGAGACAA GCCAAGCAAG CAGGAGAAGA GTAGCTTGTT TCAGATCAGC
    AAGAGCATTG GGGTCCTAAC ACCACTCTTG GGGCTCACCT GGGGTTTTGG TCTTGCCACT
    GTGTTCCAAG GAAGCAATGC TGTGTTCCAT ATTATATTTA CACTCCTCAA TGCCTTTCAG
    GGATTATTCA TTTTGCTCTT TGGATGCCTC TGGGATCAGA AGGTACAGGA AGCCTTACTA
    AAGAAGTTTT CACTGTCAAG ATGGTCTTCT CAGCACTCAA AGTCAACATC CCTAGGTTCA
    TCTACACCAG TATTTTCTAT GAGTTCTCCA ATATCAAGAA GATTTAACAA TTTATTTGGA
    AAAACAGGAA CGTACAATGT TTCCACCCCA GAAACAACCA GCTCATCCCT GGAAAACACA
    TCCAGTGCTT ACTCCTTGCT GAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe15374
    Clone ID Related Accession (Same CDS sequence) XM_005627445.3 , XM_005627445.2
    Accession Version XM_005627445.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4047bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product adhesion G protein-coupled receptor F5 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005627445.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAAATCCC CAAGGAGAAC CACATTGTGC TTCATGCTTA TTGTGATTTA TTTTTCCCAA 
    GCTACACTGA ACTTGAATTT AGAATCTATT GTTCATCCTT TGATTCTCCA TGAACATGAA
    CTAACAGAGA AGGAGCCACT AAGGGAAAAA CGAGCAGTTG CCTCCAGCAG CCCAGTACCT
    GCAGAGTACA CTGTTGATGT GGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAGCCTATC
    AAAGCTTATG TGAACAGCCT CAGTTTCCCA ATTCCAGGGA ACAGCACAGA CCTGGCCACC
    AACATTTTAA GCATTGAGGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CGGGCTATAG GTGGCCTCAG GAGAAGTGCC TCCACAATCC CACTTGTCAA
    GAGCATGACA GCTCCCTTGC AGGGCATCGT TGCAGTTGCC TGAAAGGACT ACCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGCAGAT GTTACTTTAA GAATGTCAGT CAGACTCAAT
    GTGGGCTTTC AAGAGGACCT CAAGAATTCT TCCTCTGCTC TCTATAGGTC CTACAAGACT
    GACCTGGAAA CAGCGTTCTG GAAAGGTTAC AGTACTTTAC CAGGCTTCAA ATTGGTGACT
    GTGACAGGGT TCAGGCCCGG AAGTGTGGTT GTGACATATG AGGTGAAGAC TACAACACCA
    TCACCTCAAT TGATGCATAA AGCAAATGAG CAAGTGATGC AGAACCTGAA TCAGACCTAC
    AGAATGGACT ACAGTTCCTT TCAAGCCGTT ACTAGCAATG AAACTAAGTT CACCATCATA
    CCAGAAATCA TTTTTGAAGG GGATACGGTA AACATGATGT GTGAAACCGA CGTTTTGTCC
    TCTAACGTGT CCTGGCACCA TGTTGAAAGG CACTCTGACA TCCAGAACAG CAGCAGATTC
    TCAGTTTACA CCACCGTGCT GAACAACATG ACCTCAGTCT CCCGCCTCAC GATTTACAAC
    ATCACCCAGC GGGATGCAGG TGAATATATT TGCAAGCTGA CATTAGATAT TTTTGAATAT
    GAGTCCAGAA AAAAAATAGA TGTCACGCCC ATCCGAATTC TGGCAACTGA AGAAATGAAG
    GTGACGTGCG ACAACAATCC TATATCTTTG AACTGCTGCA GTGAGATTAA TGCTAACTGG
    AGCACAATAG AATGGAAGCA GGAGGGGAAA ATAAACATTC CAGGCAACCC TGAAACTGAC
    CTAGAGTCCA GCTGCAGCAG ATACACCCTC AAGGCAGATG GAACCCAGTG TCCGAGTGGG
    TCGGCTGGAA CCACGGGCAT CTTTGTGTGT GAGTTCATCA GCACCTACGG AGCCAGAGGC
    AGTAAGAGCA TAGAAGTGAC GTTCATCTCT GTGGCCAACC TAACAATAAC CCCGGACCCA
    ATTTCTGTTT CTGAGGGACA AAACTTTTCC ATAAAGTGCA TCAGCGATGT GAGTAACTAT
    GATGAGGTAT ACTGGAACAC GTCTGCTGGA ATTAAAATAT ACCAAAAGTT TTATACCACG
    AGGAGGTTTT CTGATGGAGC AGAGTCAGTG CTGACAGTGA AGACTGTGAC CCGGGAGTGG
    AACGGAACCT ATCACTGCAT ATTTAGATAC AAGAATTCAT ACAGTGTCGT AATGAAAGAT
    GTGACCGTTT ACCCACTGCC TCTAGAGCCA AACATCATGG TTGATCCTCT GGAGGCTACA
    GTTCCCTGCA AGGGTTCCCA TCACTTCAGA TGCTGCATTG ACGAGGATGA GAACTACAGA
    GTGACTTTCC AAGTGGATTC CTTATCCTTT CCTGCAGAAA AAGAAGTTAA TGGCAAGCAA
    GTGTGCTACA AATACAAGTT CATGGCAAGG ACATCAGTTT CCTGGTGTCT AAAAAAGCTG
    GACATATTTT GTCACTTTAC CAATGCTGCT AATAATTCAG TCCGGAGCCC ATCTATGAAG
    CTTAATGTGG TTCCTGGAAA GAACATCATG TGCCGAGATC CCGTAATAGG TGTTGGGGAG
    CCTGGAAAAG TCATCCAGAA ACTCTGCCAT TTCTCAAAAG TACCAAACAT CTCTGGAGGT
    CCTACTGGAG GGATCATAAC TTACAAATGT GTAGGCTCCC AATGGGAGGT GAAGAAAAAT
    GACTGCATCT CTGCCCCAAT AAATAGTCTG CTCCAGCTGG CCAAGGCTTT GACCAAGAGC
    CCCACTCAGG ATAAGAAGCT CCCAACATAC CTAAAGGATC TTTCTATTAG CACAGGCAAG
    GTAAAACATG AAGTCAGCTC ATTTCCTGGG AGCCTGGGAG CCATCGTTAA CATCCTTGAT
    TTGCTTTCAA CAGTTACAAC CCAAGTGAAT TCAGAGATGA TGATGAACGT TCTCTCTACG
    GTTAACACTG TCCTCAGCAA GCCCATGTTG AGTACCTGGA AGGTGTCCCA ACAGCAACAG
    ACCAACCAGA GCTCACAGCT ACTGGATTCA GTAGAAAGAT TTTCCCGAGC ATTACAGACA
    GAAGATAGCA CCTTTTTATC CATCAACCAA ACTAACGTGC AGATGAGGAG CATGGTAATA
    AAAACTGGCC ATCCAAAAAC CTACAAACAG AGCTTTGTTT TCTCAGACTT GGACCTCTGG
    GGCAATGTGG CCATTGACAA GTGCGAGGTG GGAAGCCTGC AGCAAGATTC ATCTATTGTC
    ACCGTGGCTT TCCCAACTCT CCAAACCATC CTCACTCAGG ATGTTCAAGG AAAGAATTTT
    GCCAACAGCT TAGTGATGAC AACCACTGTT AGCCACAACA TAACCATGCC ATTTAGGATT
    TCAATGACTT TTAAGAACAT CAATCCTTCA GGTGGGGTAC CACAATGTGT CTACTGGAAT
    TTCAACCTCG CCAACCACAC AGGGGGTTGG GATAGTAGTG GGTGTTATGT GCACAATGTC
    GATGGGGACA GTGTCTCCTG CATCTGTGAC CACCTAACAT CATTCTCCAT CCTCATGTCC
    CCCGACTCTC CGTACCCTGG TTCCCTCCTG GGAATACTAC TGGATATCAT TTCCTACATT
    GGGTTATGCT TTTCCATCTT GAGCTTAGCA GCCTGTCTAG TGGTGGAAGC TGTGGTGTGG
    AAATCAGTGA CCAAGAATCG GACTTCCTAT ATGCGCCACA CCTGCATAGT GAACATTGCA
    GCCTCTCTTC TGGTTGCTGA CATCTGGTTC ATTGTAGCTG CTGCCATCCA CGACCATTTT
    TACCCACTCA ATGAGACAGC CTGTGTGGCC GCCACCTTTT TCATCCACTT CTTCTACCTC
    AGCGTCTTTT TCTGGATGCT GACTCTGGGC CTCATGCTCT TCTACCGCCT GGTTTTCATC
    CTGCATGACA CAAGCAAGTC CATTCAGAAG ACTATTGCAT TTTCTCTTGG CTATGGCTGC
    CCTCTTGTCA TCTCAGTCAT CACAGTGGGG GCCACCCAAC CCCAAGAAGT CTACACCAGG
    AAGAATGCCT GCTGGCTCAA CTGGGAGGAT ACCAAGGCCC TGCTGGCCTT TGTCATCCCA
    GCACTGATCA TCGTGGTGGT GAACATGACC ATCACGGTTG TGGTCATCTC CAAGATCCTG
    AGACCCTCCA TTGGAGACAA GCCAAGCAAG CAGGAGAAGA GTAGCTTGTT TCAGATCAGC
    AAGAGCATTG GGGTCCTAAC ACCACTCTTG GGGCTCACCT GGGGTTTTGG TCTTGCCACT
    GTGTTCCAAG GAAGCAATGC TGTGTTCCAT ATTATATTTA CACTCCTCAA TGCCTTTCAG
    GGATTATTCA TTTTGCTCTT TGGATGCCTC TGGGATCAGA AGGTACAGGA AGCCTTACTA
    AAGAAGTTTT CACTGTCAAG ATGGTCTTCT CAGCACTCAA AGTCAACATC CCTAGGTTCA
    TCTACACCAG TATTTTCTAT GAGTTCTCCA ATATCAAGAA GATTTAACAA TTTATTTGGA
    AAAACAGGAA CGTACAATGT TTCCACCCCA GAAACAACCA GCTCATCCCT GGAAAACACA
    TCCAGTGCTT ACTCCTTGCT GAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005627502.1
    CDS396..4442
    Translation

    Target ORF information:

    RefSeq Version XM_005627445.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005627445.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAAATCCC CAAGGAGAAC CACATTGTGC TTCATGCTTA TTGTGATTTA TTTTTCCCAA 
    GCTACACTGA ACTTGAATTT AGAATCTATT GTTCATCCTT TGATTCTCCA TGAACATGAA
    CTAACAGAGA AGGAGCCACT AAGGGAAAAA CGAGCAGTTG CCTCCAGCAG CCCAGTACCT
    GCAGAGTACA CTGTTGATGT GGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAGCCTATC
    AAAGCTTATG TGAACAGCCT CAGTTTCCCA ATTCCAGGGA ACAGCACAGA CCTGGCCACC
    AACATTTTAA GCATTGAGGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CGGGCTATAG GTGGCCTCAG GAGAAGTGCC TCCACAATCC CACTTGTCAA
    GAGCATGACA GCTCCCTTGC AGGGCATCGT TGCAGTTGCC TGAAAGGACT ACCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGCAGAT GTTACTTTAA GAATGTCAGT CAGACTCAAT
    GTGGGCTTTC AAGAGGACCT CAAGAATTCT TCCTCTGCTC TCTATAGGTC CTACAAGACT
    GACCTGGAAA CAGCGTTCTG GAAAGGTTAC AGTACTTTAC CAGGCTTCAA ATTGGTGACT
    GTGACAGGGT TCAGGCCCGG AAGTGTGGTT GTGACATATG AGGTGAAGAC TACAACACCA
    TCACCTCAAT TGATGCATAA AGCAAATGAG CAAGTGATGC AGAACCTGAA TCAGACCTAC
    AGAATGGACT ACAGTTCCTT TCAAGCCGTT ACTAGCAATG AAACTAAGTT CACCATCATA
    CCAGAAATCA TTTTTGAAGG GGATACGGTA AACATGATGT GTGAAACCGA CGTTTTGTCC
    TCTAACGTGT CCTGGCACCA TGTTGAAAGG CACTCTGACA TCCAGAACAG CAGCAGATTC
    TCAGTTTACA CCACCGTGCT GAACAACATG ACCTCAGTCT CCCGCCTCAC GATTTACAAC
    ATCACCCAGC GGGATGCAGG TGAATATATT TGCAAGCTGA CATTAGATAT TTTTGAATAT
    GAGTCCAGAA AAAAAATAGA TGTCACGCCC ATCCGAATTC TGGCAACTGA AGAAATGAAG
    GTGACGTGCG ACAACAATCC TATATCTTTG AACTGCTGCA GTGAGATTAA TGCTAACTGG
    AGCACAATAG AATGGAAGCA GGAGGGGAAA ATAAACATTC CAGGCAACCC TGAAACTGAC
    CTAGAGTCCA GCTGCAGCAG ATACACCCTC AAGGCAGATG GAACCCAGTG TCCGAGTGGG
    TCGGCTGGAA CCACGGGCAT CTTTGTGTGT GAGTTCATCA GCACCTACGG AGCCAGAGGC
    AGTAAGAGCA TAGAAGTGAC GTTCATCTCT GTGGCCAACC TAACAATAAC CCCGGACCCA
    ATTTCTGTTT CTGAGGGACA AAACTTTTCC ATAAAGTGCA TCAGCGATGT GAGTAACTAT
    GATGAGGTAT ACTGGAACAC GTCTGCTGGA ATTAAAATAT ACCAAAAGTT TTATACCACG
    AGGAGGTTTT CTGATGGAGC AGAGTCAGTG CTGACAGTGA AGACTGTGAC CCGGGAGTGG
    AACGGAACCT ATCACTGCAT ATTTAGATAC AAGAATTCAT ACAGTGTCGT AATGAAAGAT
    GTGACCGTTT ACCCACTGCC TCTAGAGCCA AACATCATGG TTGATCCTCT GGAGGCTACA
    GTTCCCTGCA AGGGTTCCCA TCACTTCAGA TGCTGCATTG ACGAGGATGA GAACTACAGA
    GTGACTTTCC AAGTGGATTC CTTATCCTTT CCTGCAGAAA AAGAAGTTAA TGGCAAGCAA
    GTGTGCTACA AATACAAGTT CATGGCAAGG ACATCAGTTT CCTGGTGTCT AAAAAAGCTG
    GACATATTTT GTCACTTTAC CAATGCTGCT AATAATTCAG TCCGGAGCCC ATCTATGAAG
    CTTAATGTGG TTCCTGGAAA GAACATCATG TGCCGAGATC CCGTAATAGG TGTTGGGGAG
    CCTGGAAAAG TCATCCAGAA ACTCTGCCAT TTCTCAAAAG TACCAAACAT CTCTGGAGGT
    CCTACTGGAG GGATCATAAC TTACAAATGT GTAGGCTCCC AATGGGAGGT GAAGAAAAAT
    GACTGCATCT CTGCCCCAAT AAATAGTCTG CTCCAGCTGG CCAAGGCTTT GACCAAGAGC
    CCCACTCAGG ATAAGAAGCT CCCAACATAC CTAAAGGATC TTTCTATTAG CACAGGCAAG
    GTAAAACATG AAGTCAGCTC ATTTCCTGGG AGCCTGGGAG CCATCGTTAA CATCCTTGAT
    TTGCTTTCAA CAGTTACAAC CCAAGTGAAT TCAGAGATGA TGATGAACGT TCTCTCTACG
    GTTAACACTG TCCTCAGCAA GCCCATGTTG AGTACCTGGA AGGTGTCCCA ACAGCAACAG
    ACCAACCAGA GCTCACAGCT ACTGGATTCA GTAGAAAGAT TTTCCCGAGC ATTACAGACA
    GAAGATAGCA CCTTTTTATC CATCAACCAA ACTAACGTGC AGATGAGGAG CATGGTAATA
    AAAACTGGCC ATCCAAAAAC CTACAAACAG AGCTTTGTTT TCTCAGACTT GGACCTCTGG
    GGCAATGTGG CCATTGACAA GTGCGAGGTG GGAAGCCTGC AGCAAGATTC ATCTATTGTC
    ACCGTGGCTT TCCCAACTCT CCAAACCATC CTCACTCAGG ATGTTCAAGG AAAGAATTTT
    GCCAACAGCT TAGTGATGAC AACCACTGTT AGCCACAACA TAACCATGCC ATTTAGGATT
    TCAATGACTT TTAAGAACAT CAATCCTTCA GGTGGGGTAC CACAATGTGT CTACTGGAAT
    TTCAACCTCG CCAACCACAC AGGGGGTTGG GATAGTAGTG GGTGTTATGT GCACAATGTC
    GATGGGGACA GTGTCTCCTG CATCTGTGAC CACCTAACAT CATTCTCCAT CCTCATGTCC
    CCCGACTCTC CGTACCCTGG TTCCCTCCTG GGAATACTAC TGGATATCAT TTCCTACATT
    GGGTTATGCT TTTCCATCTT GAGCTTAGCA GCCTGTCTAG TGGTGGAAGC TGTGGTGTGG
    AAATCAGTGA CCAAGAATCG GACTTCCTAT ATGCGCCACA CCTGCATAGT GAACATTGCA
    GCCTCTCTTC TGGTTGCTGA CATCTGGTTC ATTGTAGCTG CTGCCATCCA CGACCATTTT
    TACCCACTCA ATGAGACAGC CTGTGTGGCC GCCACCTTTT TCATCCACTT CTTCTACCTC
    AGCGTCTTTT TCTGGATGCT GACTCTGGGC CTCATGCTCT TCTACCGCCT GGTTTTCATC
    CTGCATGACA CAAGCAAGTC CATTCAGAAG ACTATTGCAT TTTCTCTTGG CTATGGCTGC
    CCTCTTGTCA TCTCAGTCAT CACAGTGGGG GCCACCCAAC CCCAAGAAGT CTACACCAGG
    AAGAATGCCT GCTGGCTCAA CTGGGAGGAT ACCAAGGCCC TGCTGGCCTT TGTCATCCCA
    GCACTGATCA TCGTGGTGGT GAACATGACC ATCACGGTTG TGGTCATCTC CAAGATCCTG
    AGACCCTCCA TTGGAGACAA GCCAAGCAAG CAGGAGAAGA GTAGCTTGTT TCAGATCAGC
    AAGAGCATTG GGGTCCTAAC ACCACTCTTG GGGCTCACCT GGGGTTTTGG TCTTGCCACT
    GTGTTCCAAG GAAGCAATGC TGTGTTCCAT ATTATATTTA CACTCCTCAA TGCCTTTCAG
    GGATTATTCA TTTTGCTCTT TGGATGCCTC TGGGATCAGA AGGTACAGGA AGCCTTACTA
    AAGAAGTTTT CACTGTCAAG ATGGTCTTCT CAGCACTCAA AGTCAACATC CCTAGGTTCA
    TCTACACCAG TATTTTCTAT GAGTTCTCCA ATATCAAGAA GATTTAACAA TTTATTTGGA
    AAAACAGGAA CGTACAATGT TTCCACCCCA GAAACAACCA GCTCATCCCT GGAAAACACA
    TCCAGTGCTT ACTCCTTGCT GAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe15375
    Clone ID Related Accession (Same CDS sequence) XM_532160.6 , XM_532160.5
    Accession Version XM_532160.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4044bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product adhesion G protein-coupled receptor F5 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_532160.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAAATCCC CAAGGAGAAC CACATTGTGC TTCATGCTTA TTGTGATTTA TTTTTCCCAA 
    GCTACACTGA ACTTGAATTT AGAATCTATT GTTCATCCTT TGATTCTCCA TGAACATGAA
    CTAACAGAGA AGGAGCCACT AAGGGAAAAA CGAGCAGTTG CCTCCAGCAG CCCAGTACCT
    GCAGAGTACA CTGTTGATGT GGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAGCCTATC
    AAAGCTTATG TGAACAGCCT CAGTTTCCCA ATTCCAGGGA ACAGCACAGA CCTGGCCACC
    AACATTTTAA GCATTGAGGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CGGGCTATAG GTGGCCTCAG GAGAAGTGCC TCCACAATCC CACTTGTCAA
    GAGCATGACA GCTCCCTTGC AGGGCATCGT TGCAGTTGCC TGAAAGGACT ACCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGATGTT ACTTTAAGAA TGTCAGTCAG ACTCAATGTG
    GGCTTTCAAG AGGACCTCAA GAATTCTTCC TCTGCTCTCT ATAGGTCCTA CAAGACTGAC
    CTGGAAACAG CGTTCTGGAA AGGTTACAGT ACTTTACCAG GCTTCAAATT GGTGACTGTG
    ACAGGGTTCA GGCCCGGAAG TGTGGTTGTG ACATATGAGG TGAAGACTAC AACACCATCA
    CCTCAATTGA TGCATAAAGC AAATGAGCAA GTGATGCAGA ACCTGAATCA GACCTACAGA
    ATGGACTACA GTTCCTTTCA AGCCGTTACT AGCAATGAAA CTAAGTTCAC CATCATACCA
    GAAATCATTT TTGAAGGGGA TACGGTAAAC ATGATGTGTG AAACCGACGT TTTGTCCTCT
    AACGTGTCCT GGCACCATGT TGAAAGGCAC TCTGACATCC AGAACAGCAG CAGATTCTCA
    GTTTACACCA CCGTGCTGAA CAACATGACC TCAGTCTCCC GCCTCACGAT TTACAACATC
    ACCCAGCGGG ATGCAGGTGA ATATATTTGC AAGCTGACAT TAGATATTTT TGAATATGAG
    TCCAGAAAAA AAATAGATGT CACGCCCATC CGAATTCTGG CAACTGAAGA AATGAAGGTG
    ACGTGCGACA ACAATCCTAT ATCTTTGAAC TGCTGCAGTG AGATTAATGC TAACTGGAGC
    ACAATAGAAT GGAAGCAGGA GGGGAAAATA AACATTCCAG GCAACCCTGA AACTGACCTA
    GAGTCCAGCT GCAGCAGATA CACCCTCAAG GCAGATGGAA CCCAGTGTCC GAGTGGGTCG
    GCTGGAACCA CGGGCATCTT TGTGTGTGAG TTCATCAGCA CCTACGGAGC CAGAGGCAGT
    AAGAGCATAG AAGTGACGTT CATCTCTGTG GCCAACCTAA CAATAACCCC GGACCCAATT
    TCTGTTTCTG AGGGACAAAA CTTTTCCATA AAGTGCATCA GCGATGTGAG TAACTATGAT
    GAGGTATACT GGAACACGTC TGCTGGAATT AAAATATACC AAAAGTTTTA TACCACGAGG
    AGGTTTTCTG ATGGAGCAGA GTCAGTGCTG ACAGTGAAGA CTGTGACCCG GGAGTGGAAC
    GGAACCTATC ACTGCATATT TAGATACAAG AATTCATACA GTGTCGTAAT GAAAGATGTG
    ACCGTTTACC CACTGCCTCT AGAGCCAAAC ATCATGGTTG ATCCTCTGGA GGCTACAGTT
    CCCTGCAAGG GTTCCCATCA CTTCAGATGC TGCATTGACG AGGATGAGAA CTACAGAGTG
    ACTTTCCAAG TGGATTCCTT ATCCTTTCCT GCAGAAAAAG AAGTTAATGG CAAGCAAGTG
    TGCTACAAAT ACAAGTTCAT GGCAAGGACA TCAGTTTCCT GGTGTCTAAA AAAGCTGGAC
    ATATTTTGTC ACTTTACCAA TGCTGCTAAT AATTCAGTCC GGAGCCCATC TATGAAGCTT
    AATGTGGTTC CTGGAAAGAA CATCATGTGC CGAGATCCCG TAATAGGTGT TGGGGAGCCT
    GGAAAAGTCA TCCAGAAACT CTGCCATTTC TCAAAAGTAC CAAACATCTC TGGAGGTCCT
    ACTGGAGGGA TCATAACTTA CAAATGTGTA GGCTCCCAAT GGGAGGTGAA GAAAAATGAC
    TGCATCTCTG CCCCAATAAA TAGTCTGCTC CAGCTGGCCA AGGCTTTGAC CAAGAGCCCC
    ACTCAGGATA AGAAGCTCCC AACATACCTA AAGGATCTTT CTATTAGCAC AGGCAAGGTA
    AAACATGAAG TCAGCTCATT TCCTGGGAGC CTGGGAGCCA TCGTTAACAT CCTTGATTTG
    CTTTCAACAG TTACAACCCA AGTGAATTCA GAGATGATGA TGAACGTTCT CTCTACGGTT
    AACACTGTCC TCAGCAAGCC CATGTTGAGT ACCTGGAAGG TGTCCCAACA GCAACAGACC
    AACCAGAGCT CACAGCTACT GGATTCAGTA GAAAGATTTT CCCGAGCATT ACAGACAGAA
    GATAGCACCT TTTTATCCAT CAACCAAACT AACGTGCAGA TGAGGAGCAT GGTAATAAAA
    ACTGGCCATC CAAAAACCTA CAAACAGAGC TTTGTTTTCT CAGACTTGGA CCTCTGGGGC
    AATGTGGCCA TTGACAAGTG CGAGGTGGGA AGCCTGCAGC AAGATTCATC TATTGTCACC
    GTGGCTTTCC CAACTCTCCA AACCATCCTC ACTCAGGATG TTCAAGGAAA GAATTTTGCC
    AACAGCTTAG TGATGACAAC CACTGTTAGC CACAACATAA CCATGCCATT TAGGATTTCA
    ATGACTTTTA AGAACATCAA TCCTTCAGGT GGGGTACCAC AATGTGTCTA CTGGAATTTC
    AACCTCGCCA ACCACACAGG GGGTTGGGAT AGTAGTGGGT GTTATGTGCA CAATGTCGAT
    GGGGACAGTG TCTCCTGCAT CTGTGACCAC CTAACATCAT TCTCCATCCT CATGTCCCCC
    GACTCTCCGT ACCCTGGTTC CCTCCTGGGA ATACTACTGG ATATCATTTC CTACATTGGG
    TTATGCTTTT CCATCTTGAG CTTAGCAGCC TGTCTAGTGG TGGAAGCTGT GGTGTGGAAA
    TCAGTGACCA AGAATCGGAC TTCCTATATG CGCCACACCT GCATAGTGAA CATTGCAGCC
    TCTCTTCTGG TTGCTGACAT CTGGTTCATT GTAGCTGCTG CCATCCACGA CCATTTTTAC
    CCACTCAATG AGACAGCCTG TGTGGCCGCC ACCTTTTTCA TCCACTTCTT CTACCTCAGC
    GTCTTTTTCT GGATGCTGAC TCTGGGCCTC ATGCTCTTCT ACCGCCTGGT TTTCATCCTG
    CATGACACAA GCAAGTCCAT TCAGAAGACT ATTGCATTTT CTCTTGGCTA TGGCTGCCCT
    CTTGTCATCT CAGTCATCAC AGTGGGGGCC ACCCAACCCC AAGAAGTCTA CACCAGGAAG
    AATGCCTGCT GGCTCAACTG GGAGGATACC AAGGCCCTGC TGGCCTTTGT CATCCCAGCA
    CTGATCATCG TGGTGGTGAA CATGACCATC ACGGTTGTGG TCATCTCCAA GATCCTGAGA
    CCCTCCATTG GAGACAAGCC AAGCAAGCAG GAGAAGAGTA GCTTGTTTCA GATCAGCAAG
    AGCATTGGGG TCCTAACACC ACTCTTGGGG CTCACCTGGG GTTTTGGTCT TGCCACTGTG
    TTCCAAGGAA GCAATGCTGT GTTCCATATT ATATTTACAC TCCTCAATGC CTTTCAGGGA
    TTATTCATTT TGCTCTTTGG ATGCCTCTGG GATCAGAAGG TACAGGAAGC CTTACTAAAG
    AAGTTTTCAC TGTCAAGATG GTCTTCTCAG CACTCAAAGT CAACATCCCT AGGTTCATCT
    ACACCAGTAT TTTCTATGAG TTCTCCAATA TCAAGAAGAT TTAACAATTT ATTTGGAAAA
    ACAGGAACGT ACAATGTTTC CACCCCAGAA ACAACCAGCT CATCCCTGGA AAACACATCC
    AGTGCTTACT CCTTGCTGAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_532160.3
    CDS409..4452
    Translation

    Target ORF information:

    RefSeq Version XM_532160.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_532160.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAAATCCC CAAGGAGAAC CACATTGTGC TTCATGCTTA TTGTGATTTA TTTTTCCCAA 
    GCTACACTGA ACTTGAATTT AGAATCTATT GTTCATCCTT TGATTCTCCA TGAACATGAA
    CTAACAGAGA AGGAGCCACT AAGGGAAAAA CGAGCAGTTG CCTCCAGCAG CCCAGTACCT
    GCAGAGTACA CTGTTGATGT GGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAGCCTATC
    AAAGCTTATG TGAACAGCCT CAGTTTCCCA ATTCCAGGGA ACAGCACAGA CCTGGCCACC
    AACATTTTAA GCATTGAGGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CGGGCTATAG GTGGCCTCAG GAGAAGTGCC TCCACAATCC CACTTGTCAA
    GAGCATGACA GCTCCCTTGC AGGGCATCGT TGCAGTTGCC TGAAAGGACT ACCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGATGTT ACTTTAAGAA TGTCAGTCAG ACTCAATGTG
    GGCTTTCAAG AGGACCTCAA GAATTCTTCC TCTGCTCTCT ATAGGTCCTA CAAGACTGAC
    CTGGAAACAG CGTTCTGGAA AGGTTACAGT ACTTTACCAG GCTTCAAATT GGTGACTGTG
    ACAGGGTTCA GGCCCGGAAG TGTGGTTGTG ACATATGAGG TGAAGACTAC AACACCATCA
    CCTCAATTGA TGCATAAAGC AAATGAGCAA GTGATGCAGA ACCTGAATCA GACCTACAGA
    ATGGACTACA GTTCCTTTCA AGCCGTTACT AGCAATGAAA CTAAGTTCAC CATCATACCA
    GAAATCATTT TTGAAGGGGA TACGGTAAAC ATGATGTGTG AAACCGACGT TTTGTCCTCT
    AACGTGTCCT GGCACCATGT TGAAAGGCAC TCTGACATCC AGAACAGCAG CAGATTCTCA
    GTTTACACCA CCGTGCTGAA CAACATGACC TCAGTCTCCC GCCTCACGAT TTACAACATC
    ACCCAGCGGG ATGCAGGTGA ATATATTTGC AAGCTGACAT TAGATATTTT TGAATATGAG
    TCCAGAAAAA AAATAGATGT CACGCCCATC CGAATTCTGG CAACTGAAGA AATGAAGGTG
    ACGTGCGACA ACAATCCTAT ATCTTTGAAC TGCTGCAGTG AGATTAATGC TAACTGGAGC
    ACAATAGAAT GGAAGCAGGA GGGGAAAATA AACATTCCAG GCAACCCTGA AACTGACCTA
    GAGTCCAGCT GCAGCAGATA CACCCTCAAG GCAGATGGAA CCCAGTGTCC GAGTGGGTCG
    GCTGGAACCA CGGGCATCTT TGTGTGTGAG TTCATCAGCA CCTACGGAGC CAGAGGCAGT
    AAGAGCATAG AAGTGACGTT CATCTCTGTG GCCAACCTAA CAATAACCCC GGACCCAATT
    TCTGTTTCTG AGGGACAAAA CTTTTCCATA AAGTGCATCA GCGATGTGAG TAACTATGAT
    GAGGTATACT GGAACACGTC TGCTGGAATT AAAATATACC AAAAGTTTTA TACCACGAGG
    AGGTTTTCTG ATGGAGCAGA GTCAGTGCTG ACAGTGAAGA CTGTGACCCG GGAGTGGAAC
    GGAACCTATC ACTGCATATT TAGATACAAG AATTCATACA GTGTCGTAAT GAAAGATGTG
    ACCGTTTACC CACTGCCTCT AGAGCCAAAC ATCATGGTTG ATCCTCTGGA GGCTACAGTT
    CCCTGCAAGG GTTCCCATCA CTTCAGATGC TGCATTGACG AGGATGAGAA CTACAGAGTG
    ACTTTCCAAG TGGATTCCTT ATCCTTTCCT GCAGAAAAAG AAGTTAATGG CAAGCAAGTG
    TGCTACAAAT ACAAGTTCAT GGCAAGGACA TCAGTTTCCT GGTGTCTAAA AAAGCTGGAC
    ATATTTTGTC ACTTTACCAA TGCTGCTAAT AATTCAGTCC GGAGCCCATC TATGAAGCTT
    AATGTGGTTC CTGGAAAGAA CATCATGTGC CGAGATCCCG TAATAGGTGT TGGGGAGCCT
    GGAAAAGTCA TCCAGAAACT CTGCCATTTC TCAAAAGTAC CAAACATCTC TGGAGGTCCT
    ACTGGAGGGA TCATAACTTA CAAATGTGTA GGCTCCCAAT GGGAGGTGAA GAAAAATGAC
    TGCATCTCTG CCCCAATAAA TAGTCTGCTC CAGCTGGCCA AGGCTTTGAC CAAGAGCCCC
    ACTCAGGATA AGAAGCTCCC AACATACCTA AAGGATCTTT CTATTAGCAC AGGCAAGGTA
    AAACATGAAG TCAGCTCATT TCCTGGGAGC CTGGGAGCCA TCGTTAACAT CCTTGATTTG
    CTTTCAACAG TTACAACCCA AGTGAATTCA GAGATGATGA TGAACGTTCT CTCTACGGTT
    AACACTGTCC TCAGCAAGCC CATGTTGAGT ACCTGGAAGG TGTCCCAACA GCAACAGACC
    AACCAGAGCT CACAGCTACT GGATTCAGTA GAAAGATTTT CCCGAGCATT ACAGACAGAA
    GATAGCACCT TTTTATCCAT CAACCAAACT AACGTGCAGA TGAGGAGCAT GGTAATAAAA
    ACTGGCCATC CAAAAACCTA CAAACAGAGC TTTGTTTTCT CAGACTTGGA CCTCTGGGGC
    AATGTGGCCA TTGACAAGTG CGAGGTGGGA AGCCTGCAGC AAGATTCATC TATTGTCACC
    GTGGCTTTCC CAACTCTCCA AACCATCCTC ACTCAGGATG TTCAAGGAAA GAATTTTGCC
    AACAGCTTAG TGATGACAAC CACTGTTAGC CACAACATAA CCATGCCATT TAGGATTTCA
    ATGACTTTTA AGAACATCAA TCCTTCAGGT GGGGTACCAC AATGTGTCTA CTGGAATTTC
    AACCTCGCCA ACCACACAGG GGGTTGGGAT AGTAGTGGGT GTTATGTGCA CAATGTCGAT
    GGGGACAGTG TCTCCTGCAT CTGTGACCAC CTAACATCAT TCTCCATCCT CATGTCCCCC
    GACTCTCCGT ACCCTGGTTC CCTCCTGGGA ATACTACTGG ATATCATTTC CTACATTGGG
    TTATGCTTTT CCATCTTGAG CTTAGCAGCC TGTCTAGTGG TGGAAGCTGT GGTGTGGAAA
    TCAGTGACCA AGAATCGGAC TTCCTATATG CGCCACACCT GCATAGTGAA CATTGCAGCC
    TCTCTTCTGG TTGCTGACAT CTGGTTCATT GTAGCTGCTG CCATCCACGA CCATTTTTAC
    CCACTCAATG AGACAGCCTG TGTGGCCGCC ACCTTTTTCA TCCACTTCTT CTACCTCAGC
    GTCTTTTTCT GGATGCTGAC TCTGGGCCTC ATGCTCTTCT ACCGCCTGGT TTTCATCCTG
    CATGACACAA GCAAGTCCAT TCAGAAGACT ATTGCATTTT CTCTTGGCTA TGGCTGCCCT
    CTTGTCATCT CAGTCATCAC AGTGGGGGCC ACCCAACCCC AAGAAGTCTA CACCAGGAAG
    AATGCCTGCT GGCTCAACTG GGAGGATACC AAGGCCCTGC TGGCCTTTGT CATCCCAGCA
    CTGATCATCG TGGTGGTGAA CATGACCATC ACGGTTGTGG TCATCTCCAA GATCCTGAGA
    CCCTCCATTG GAGACAAGCC AAGCAAGCAG GAGAAGAGTA GCTTGTTTCA GATCAGCAAG
    AGCATTGGGG TCCTAACACC ACTCTTGGGG CTCACCTGGG GTTTTGGTCT TGCCACTGTG
    TTCCAAGGAA GCAATGCTGT GTTCCATATT ATATTTACAC TCCTCAATGC CTTTCAGGGA
    TTATTCATTT TGCTCTTTGG ATGCCTCTGG GATCAGAAGG TACAGGAAGC CTTACTAAAG
    AAGTTTTCAC TGTCAAGATG GTCTTCTCAG CACTCAAAGT CAACATCCCT AGGTTCATCT
    ACACCAGTAT TTTCTATGAG TTCTCCAATA TCAAGAAGAT TTAACAATTT ATTTGGAAAA
    ACAGGAACGT ACAATGTTTC CACCCCAGAA ACAACCAGCT CATCCCTGGA AAACACATCC
    AGTGCTTACT CCTTGCTGAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe15375
    Clone ID Related Accession (Same CDS sequence) XM_532160.6 , XM_532160.5
    Accession Version XM_532160.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4044bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product adhesion G protein-coupled receptor F5 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_532160.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAAATCCC CAAGGAGAAC CACATTGTGC TTCATGCTTA TTGTGATTTA TTTTTCCCAA 
    GCTACACTGA ACTTGAATTT AGAATCTATT GTTCATCCTT TGATTCTCCA TGAACATGAA
    CTAACAGAGA AGGAGCCACT AAGGGAAAAA CGAGCAGTTG CCTCCAGCAG CCCAGTACCT
    GCAGAGTACA CTGTTGATGT GGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAGCCTATC
    AAAGCTTATG TGAACAGCCT CAGTTTCCCA ATTCCAGGGA ACAGCACAGA CCTGGCCACC
    AACATTTTAA GCATTGAGGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CGGGCTATAG GTGGCCTCAG GAGAAGTGCC TCCACAATCC CACTTGTCAA
    GAGCATGACA GCTCCCTTGC AGGGCATCGT TGCAGTTGCC TGAAAGGACT ACCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGATGTT ACTTTAAGAA TGTCAGTCAG ACTCAATGTG
    GGCTTTCAAG AGGACCTCAA GAATTCTTCC TCTGCTCTCT ATAGGTCCTA CAAGACTGAC
    CTGGAAACAG CGTTCTGGAA AGGTTACAGT ACTTTACCAG GCTTCAAATT GGTGACTGTG
    ACAGGGTTCA GGCCCGGAAG TGTGGTTGTG ACATATGAGG TGAAGACTAC AACACCATCA
    CCTCAATTGA TGCATAAAGC AAATGAGCAA GTGATGCAGA ACCTGAATCA GACCTACAGA
    ATGGACTACA GTTCCTTTCA AGCCGTTACT AGCAATGAAA CTAAGTTCAC CATCATACCA
    GAAATCATTT TTGAAGGGGA TACGGTAAAC ATGATGTGTG AAACCGACGT TTTGTCCTCT
    AACGTGTCCT GGCACCATGT TGAAAGGCAC TCTGACATCC AGAACAGCAG CAGATTCTCA
    GTTTACACCA CCGTGCTGAA CAACATGACC TCAGTCTCCC GCCTCACGAT TTACAACATC
    ACCCAGCGGG ATGCAGGTGA ATATATTTGC AAGCTGACAT TAGATATTTT TGAATATGAG
    TCCAGAAAAA AAATAGATGT CACGCCCATC CGAATTCTGG CAACTGAAGA AATGAAGGTG
    ACGTGCGACA ACAATCCTAT ATCTTTGAAC TGCTGCAGTG AGATTAATGC TAACTGGAGC
    ACAATAGAAT GGAAGCAGGA GGGGAAAATA AACATTCCAG GCAACCCTGA AACTGACCTA
    GAGTCCAGCT GCAGCAGATA CACCCTCAAG GCAGATGGAA CCCAGTGTCC GAGTGGGTCG
    GCTGGAACCA CGGGCATCTT TGTGTGTGAG TTCATCAGCA CCTACGGAGC CAGAGGCAGT
    AAGAGCATAG AAGTGACGTT CATCTCTGTG GCCAACCTAA CAATAACCCC GGACCCAATT
    TCTGTTTCTG AGGGACAAAA CTTTTCCATA AAGTGCATCA GCGATGTGAG TAACTATGAT
    GAGGTATACT GGAACACGTC TGCTGGAATT AAAATATACC AAAAGTTTTA TACCACGAGG
    AGGTTTTCTG ATGGAGCAGA GTCAGTGCTG ACAGTGAAGA CTGTGACCCG GGAGTGGAAC
    GGAACCTATC ACTGCATATT TAGATACAAG AATTCATACA GTGTCGTAAT GAAAGATGTG
    ACCGTTTACC CACTGCCTCT AGAGCCAAAC ATCATGGTTG ATCCTCTGGA GGCTACAGTT
    CCCTGCAAGG GTTCCCATCA CTTCAGATGC TGCATTGACG AGGATGAGAA CTACAGAGTG
    ACTTTCCAAG TGGATTCCTT ATCCTTTCCT GCAGAAAAAG AAGTTAATGG CAAGCAAGTG
    TGCTACAAAT ACAAGTTCAT GGCAAGGACA TCAGTTTCCT GGTGTCTAAA AAAGCTGGAC
    ATATTTTGTC ACTTTACCAA TGCTGCTAAT AATTCAGTCC GGAGCCCATC TATGAAGCTT
    AATGTGGTTC CTGGAAAGAA CATCATGTGC CGAGATCCCG TAATAGGTGT TGGGGAGCCT
    GGAAAAGTCA TCCAGAAACT CTGCCATTTC TCAAAAGTAC CAAACATCTC TGGAGGTCCT
    ACTGGAGGGA TCATAACTTA CAAATGTGTA GGCTCCCAAT GGGAGGTGAA GAAAAATGAC
    TGCATCTCTG CCCCAATAAA TAGTCTGCTC CAGCTGGCCA AGGCTTTGAC CAAGAGCCCC
    ACTCAGGATA AGAAGCTCCC AACATACCTA AAGGATCTTT CTATTAGCAC AGGCAAGGTA
    AAACATGAAG TCAGCTCATT TCCTGGGAGC CTGGGAGCCA TCGTTAACAT CCTTGATTTG
    CTTTCAACAG TTACAACCCA AGTGAATTCA GAGATGATGA TGAACGTTCT CTCTACGGTT
    AACACTGTCC TCAGCAAGCC CATGTTGAGT ACCTGGAAGG TGTCCCAACA GCAACAGACC
    AACCAGAGCT CACAGCTACT GGATTCAGTA GAAAGATTTT CCCGAGCATT ACAGACAGAA
    GATAGCACCT TTTTATCCAT CAACCAAACT AACGTGCAGA TGAGGAGCAT GGTAATAAAA
    ACTGGCCATC CAAAAACCTA CAAACAGAGC TTTGTTTTCT CAGACTTGGA CCTCTGGGGC
    AATGTGGCCA TTGACAAGTG CGAGGTGGGA AGCCTGCAGC AAGATTCATC TATTGTCACC
    GTGGCTTTCC CAACTCTCCA AACCATCCTC ACTCAGGATG TTCAAGGAAA GAATTTTGCC
    AACAGCTTAG TGATGACAAC CACTGTTAGC CACAACATAA CCATGCCATT TAGGATTTCA
    ATGACTTTTA AGAACATCAA TCCTTCAGGT GGGGTACCAC AATGTGTCTA CTGGAATTTC
    AACCTCGCCA ACCACACAGG GGGTTGGGAT AGTAGTGGGT GTTATGTGCA CAATGTCGAT
    GGGGACAGTG TCTCCTGCAT CTGTGACCAC CTAACATCAT TCTCCATCCT CATGTCCCCC
    GACTCTCCGT ACCCTGGTTC CCTCCTGGGA ATACTACTGG ATATCATTTC CTACATTGGG
    TTATGCTTTT CCATCTTGAG CTTAGCAGCC TGTCTAGTGG TGGAAGCTGT GGTGTGGAAA
    TCAGTGACCA AGAATCGGAC TTCCTATATG CGCCACACCT GCATAGTGAA CATTGCAGCC
    TCTCTTCTGG TTGCTGACAT CTGGTTCATT GTAGCTGCTG CCATCCACGA CCATTTTTAC
    CCACTCAATG AGACAGCCTG TGTGGCCGCC ACCTTTTTCA TCCACTTCTT CTACCTCAGC
    GTCTTTTTCT GGATGCTGAC TCTGGGCCTC ATGCTCTTCT ACCGCCTGGT TTTCATCCTG
    CATGACACAA GCAAGTCCAT TCAGAAGACT ATTGCATTTT CTCTTGGCTA TGGCTGCCCT
    CTTGTCATCT CAGTCATCAC AGTGGGGGCC ACCCAACCCC AAGAAGTCTA CACCAGGAAG
    AATGCCTGCT GGCTCAACTG GGAGGATACC AAGGCCCTGC TGGCCTTTGT CATCCCAGCA
    CTGATCATCG TGGTGGTGAA CATGACCATC ACGGTTGTGG TCATCTCCAA GATCCTGAGA
    CCCTCCATTG GAGACAAGCC AAGCAAGCAG GAGAAGAGTA GCTTGTTTCA GATCAGCAAG
    AGCATTGGGG TCCTAACACC ACTCTTGGGG CTCACCTGGG GTTTTGGTCT TGCCACTGTG
    TTCCAAGGAA GCAATGCTGT GTTCCATATT ATATTTACAC TCCTCAATGC CTTTCAGGGA
    TTATTCATTT TGCTCTTTGG ATGCCTCTGG GATCAGAAGG TACAGGAAGC CTTACTAAAG
    AAGTTTTCAC TGTCAAGATG GTCTTCTCAG CACTCAAAGT CAACATCCCT AGGTTCATCT
    ACACCAGTAT TTTCTATGAG TTCTCCAATA TCAAGAAGAT TTAACAATTT ATTTGGAAAA
    ACAGGAACGT ACAATGTTTC CACCCCAGAA ACAACCAGCT CATCCCTGGA AAACACATCC
    AGTGCTTACT CCTTGCTGAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_532160.3
    CDS396..4439
    Translation

    Target ORF information:

    RefSeq Version XM_532160.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor F5 (ADGRF5), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_532160.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAAATCCC CAAGGAGAAC CACATTGTGC TTCATGCTTA TTGTGATTTA TTTTTCCCAA 
    GCTACACTGA ACTTGAATTT AGAATCTATT GTTCATCCTT TGATTCTCCA TGAACATGAA
    CTAACAGAGA AGGAGCCACT AAGGGAAAAA CGAGCAGTTG CCTCCAGCAG CCCAGTACCT
    GCAGAGTACA CTGTTGATGT GGAGATCAGT TTTGAAAATG CCTCCTTCCT GGAGCCTATC
    AAAGCTTATG TGAACAGCCT CAGTTTCCCA ATTCCAGGGA ACAGCACAGA CCTGGCCACC
    AACATTTTAA GCATTGAGGT GACAACAGTC TGCAGACCTG CTGGAAATGA AATCTGGTGC
    TCCTGTGAGA CGGGCTATAG GTGGCCTCAG GAGAAGTGCC TCCACAATCC CACTTGTCAA
    GAGCATGACA GCTCCCTTGC AGGGCATCGT TGCAGTTGCC TGAAAGGACT ACCTCCCAAG
    GGACCTTTTT GCCAGCTCCA GGGAGATGTT ACTTTAAGAA TGTCAGTCAG ACTCAATGTG
    GGCTTTCAAG AGGACCTCAA GAATTCTTCC TCTGCTCTCT ATAGGTCCTA CAAGACTGAC
    CTGGAAACAG CGTTCTGGAA AGGTTACAGT ACTTTACCAG GCTTCAAATT GGTGACTGTG
    ACAGGGTTCA GGCCCGGAAG TGTGGTTGTG ACATATGAGG TGAAGACTAC AACACCATCA
    CCTCAATTGA TGCATAAAGC AAATGAGCAA GTGATGCAGA ACCTGAATCA GACCTACAGA
    ATGGACTACA GTTCCTTTCA AGCCGTTACT AGCAATGAAA CTAAGTTCAC CATCATACCA
    GAAATCATTT TTGAAGGGGA TACGGTAAAC ATGATGTGTG AAACCGACGT TTTGTCCTCT
    AACGTGTCCT GGCACCATGT TGAAAGGCAC TCTGACATCC AGAACAGCAG CAGATTCTCA
    GTTTACACCA CCGTGCTGAA CAACATGACC TCAGTCTCCC GCCTCACGAT TTACAACATC
    ACCCAGCGGG ATGCAGGTGA ATATATTTGC AAGCTGACAT TAGATATTTT TGAATATGAG
    TCCAGAAAAA AAATAGATGT CACGCCCATC CGAATTCTGG CAACTGAAGA AATGAAGGTG
    ACGTGCGACA ACAATCCTAT ATCTTTGAAC TGCTGCAGTG AGATTAATGC TAACTGGAGC
    ACAATAGAAT GGAAGCAGGA GGGGAAAATA AACATTCCAG GCAACCCTGA AACTGACCTA
    GAGTCCAGCT GCAGCAGATA CACCCTCAAG GCAGATGGAA CCCAGTGTCC GAGTGGGTCG
    GCTGGAACCA CGGGCATCTT TGTGTGTGAG TTCATCAGCA CCTACGGAGC CAGAGGCAGT
    AAGAGCATAG AAGTGACGTT CATCTCTGTG GCCAACCTAA CAATAACCCC GGACCCAATT
    TCTGTTTCTG AGGGACAAAA CTTTTCCATA AAGTGCATCA GCGATGTGAG TAACTATGAT
    GAGGTATACT GGAACACGTC TGCTGGAATT AAAATATACC AAAAGTTTTA TACCACGAGG
    AGGTTTTCTG ATGGAGCAGA GTCAGTGCTG ACAGTGAAGA CTGTGACCCG GGAGTGGAAC
    GGAACCTATC ACTGCATATT TAGATACAAG AATTCATACA GTGTCGTAAT GAAAGATGTG
    ACCGTTTACC CACTGCCTCT AGAGCCAAAC ATCATGGTTG ATCCTCTGGA GGCTACAGTT
    CCCTGCAAGG GTTCCCATCA CTTCAGATGC TGCATTGACG AGGATGAGAA CTACAGAGTG
    ACTTTCCAAG TGGATTCCTT ATCCTTTCCT GCAGAAAAAG AAGTTAATGG CAAGCAAGTG
    TGCTACAAAT ACAAGTTCAT GGCAAGGACA TCAGTTTCCT GGTGTCTAAA AAAGCTGGAC
    ATATTTTGTC ACTTTACCAA TGCTGCTAAT AATTCAGTCC GGAGCCCATC TATGAAGCTT
    AATGTGGTTC CTGGAAAGAA CATCATGTGC CGAGATCCCG TAATAGGTGT TGGGGAGCCT
    GGAAAAGTCA TCCAGAAACT CTGCCATTTC TCAAAAGTAC CAAACATCTC TGGAGGTCCT
    ACTGGAGGGA TCATAACTTA CAAATGTGTA GGCTCCCAAT GGGAGGTGAA GAAAAATGAC
    TGCATCTCTG CCCCAATAAA TAGTCTGCTC CAGCTGGCCA AGGCTTTGAC CAAGAGCCCC
    ACTCAGGATA AGAAGCTCCC AACATACCTA AAGGATCTTT CTATTAGCAC AGGCAAGGTA
    AAACATGAAG TCAGCTCATT TCCTGGGAGC CTGGGAGCCA TCGTTAACAT CCTTGATTTG
    CTTTCAACAG TTACAACCCA AGTGAATTCA GAGATGATGA TGAACGTTCT CTCTACGGTT
    AACACTGTCC TCAGCAAGCC CATGTTGAGT ACCTGGAAGG TGTCCCAACA GCAACAGACC
    AACCAGAGCT CACAGCTACT GGATTCAGTA GAAAGATTTT CCCGAGCATT ACAGACAGAA
    GATAGCACCT TTTTATCCAT CAACCAAACT AACGTGCAGA TGAGGAGCAT GGTAATAAAA
    ACTGGCCATC CAAAAACCTA CAAACAGAGC TTTGTTTTCT CAGACTTGGA CCTCTGGGGC
    AATGTGGCCA TTGACAAGTG CGAGGTGGGA AGCCTGCAGC AAGATTCATC TATTGTCACC
    GTGGCTTTCC CAACTCTCCA AACCATCCTC ACTCAGGATG TTCAAGGAAA GAATTTTGCC
    AACAGCTTAG TGATGACAAC CACTGTTAGC CACAACATAA CCATGCCATT TAGGATTTCA
    ATGACTTTTA AGAACATCAA TCCTTCAGGT GGGGTACCAC AATGTGTCTA CTGGAATTTC
    AACCTCGCCA ACCACACAGG GGGTTGGGAT AGTAGTGGGT GTTATGTGCA CAATGTCGAT
    GGGGACAGTG TCTCCTGCAT CTGTGACCAC CTAACATCAT TCTCCATCCT CATGTCCCCC
    GACTCTCCGT ACCCTGGTTC CCTCCTGGGA ATACTACTGG ATATCATTTC CTACATTGGG
    TTATGCTTTT CCATCTTGAG CTTAGCAGCC TGTCTAGTGG TGGAAGCTGT GGTGTGGAAA
    TCAGTGACCA AGAATCGGAC TTCCTATATG CGCCACACCT GCATAGTGAA CATTGCAGCC
    TCTCTTCTGG TTGCTGACAT CTGGTTCATT GTAGCTGCTG CCATCCACGA CCATTTTTAC
    CCACTCAATG AGACAGCCTG TGTGGCCGCC ACCTTTTTCA TCCACTTCTT CTACCTCAGC
    GTCTTTTTCT GGATGCTGAC TCTGGGCCTC ATGCTCTTCT ACCGCCTGGT TTTCATCCTG
    CATGACACAA GCAAGTCCAT TCAGAAGACT ATTGCATTTT CTCTTGGCTA TGGCTGCCCT
    CTTGTCATCT CAGTCATCAC AGTGGGGGCC ACCCAACCCC AAGAAGTCTA CACCAGGAAG
    AATGCCTGCT GGCTCAACTG GGAGGATACC AAGGCCCTGC TGGCCTTTGT CATCCCAGCA
    CTGATCATCG TGGTGGTGAA CATGACCATC ACGGTTGTGG TCATCTCCAA GATCCTGAGA
    CCCTCCATTG GAGACAAGCC AAGCAAGCAG GAGAAGAGTA GCTTGTTTCA GATCAGCAAG
    AGCATTGGGG TCCTAACACC ACTCTTGGGG CTCACCTGGG GTTTTGGTCT TGCCACTGTG
    TTCCAAGGAA GCAATGCTGT GTTCCATATT ATATTTACAC TCCTCAATGC CTTTCAGGGA
    TTATTCATTT TGCTCTTTGG ATGCCTCTGG GATCAGAAGG TACAGGAAGC CTTACTAAAG
    AAGTTTTCAC TGTCAAGATG GTCTTCTCAG CACTCAAAGT CAACATCCCT AGGTTCATCT
    ACACCAGTAT TTTCTATGAG TTCTCCAATA TCAAGAAGAT TTAACAATTT ATTTGGAAAA
    ACAGGAACGT ACAATGTTTC CACCCCAGAA ACAACCAGCT CATCCCTGGA AAACACATCC
    AGTGCTTACT CCTTGCTGAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.