RNF20 cDNA ORF clone, Canis lupus familiaris(dog)

The following RNF20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RNF20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe14708 XM_005626880.3
Latest version!
Canis lupus familiaris ring finger protein 20 (RNF20), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe14708 XM_532018.5
Latest version!
Canis lupus familiaris ring finger protein 20 (RNF20), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe14708 XM_022425660.1
Latest version!
Canis lupus familiaris ring finger protein 20 (RNF20), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe292198 XM_022425661.1
Latest version!
Canis lupus familiaris ring finger protein 20 (RNF20), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
View Old Accession Versions >>
OCe14708 XM_005626880.2 Canis lupus familiaris ring finger protein 20, E3 ubiquitin protein ligase (RNF20), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe14708 XM_532018.4 Canis lupus familiaris ring finger protein 20, E3 ubiquitin protein ligase (RNF20), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe14708
    Clone ID Related Accession (Same CDS sequence) XM_005626880.2 , XM_022425660.1 , XM_005626880.3 , XM_532018.4 , XM_532018.5
    Accession Version XM_005626880.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2928bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase BRE1A
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005626880.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTCAGGAA TTGGAAATAA AAGAGCAGCT GGAGAGCCTG GCACATCCAT GCCTCCGGAG 
    AAGAAGACAG CCGTTGAAGA TTCAGGGACC ACAGTGGAGA CAATTAAGCT TGGAGGTGTC
    TCCTCCACGG AGGAGCTAGA CATTCGAACA CTGCAGACCA AAAATCGCAA GCTGGCAGAA
    ATGCTGGATC AACGGCAGGC CATCGAAGAT GAACTCCGTG AGCACATTGA AAAACTGGAG
    CGAAGACAGG CCACTGATGA TGCCTCACTG CTGATCGTCA ACCGGTACTG GAGTCAGTTT
    GATGAAAACA TCCGTATCAT CCTTAAACGG TACGATCTGG AGCAGGGTTT GGGAGATCTC
    CTCACAGAAA GGAAAGCCCT TGTCGTGCCC GAACCAGAGC CGGACTCTGA TAGCAATCAG
    GAACGGAAAG ATGACCGAGA GAGAGGGGAA GGGCAAGAGC CAGCTTTCTC TTTCCTTGCT
    ACTTTGGCCA GCAGTTCCAG TGAAGAAATG GAATCTCAGC TGCAGGAGCG AGTGGAGTCC
    TCCCGCCGAG CTGTGTCCCA GATCGTGACT GTCTATGATA AATTACAAGA GAAAGTGGAG
    CTGTTATCAT GCAAACTAAA TAGTGGAGAT AATCTGATAG TGGAAGAGGC GGTGCAGGAG
    CTGAATTCCT TCCTTGCACA GGAGAATATG AGGCTACAGG AATTGACAGA CCTCCTTCAG
    GAGAAGCATC GCACCATGTC TCAGGAGTTC TCCAAGTTAC AGAGTAAAGT GGAGACAGCC
    GAGTCGCGAG TGTCTGTCCT AGAGTCCATG ATCGATGACC TGCAGTGGGA TATTGACAAA
    ATTCGAAAGA GGGAACAACG ACTCAACCGA CACTTAGCAG AAGTTCTTGA ACGGGTGAAT
    TCGAAAGGTT ATAAGGTCTA TGGAGCCGGG AGCAGTCTCT ATGGTGGCAC AATCACTATC
    AATGCCCGGA AGTTTGAGGA AATGAATGCA GAGCTGGAGG AGAACAAAGA GTTGGCTCAG
    AACCGTCACT GTGAGCTGGA GAAACTTCGG CAAGACTTGG AGGAGGTCAC TACGCAAAAT
    GAAAAGCTGA AGGTGGAATT GCGGAGCGCG GTGGAGGAGG CAGTTAAGGA GACTCCGGAA
    TACCGCTGTA TGCAGTCACA GTTCTCCGTC CTGTACAATG AGAGCCTGCA GTTGAAAGCG
    CACTTGGATG AGGCTCGGAC TCTGCTTCAT GGCACCAGGG GGACCCACCA GCGCCAAGTT
    GAGCTTATTG AGCGAGATGA AGTTAGTCTT CATAAGAAGC TGAGGACTGA AGTGATCCAG
    CTGGAAGATA CTCTGGCCCA GGTCCGCAAG GAGTATGAGA TGCTGAGGAT AGAATTTGAG
    CAGACCCTTG CTGCCAATGA GCAAGCAGGC CCCATAAACC GGGAGATGCG TCACCTCATC
    AGTAGCCTCC AGAATCACAA TCACCAGCTG AAAGGGGAGG TCCTAAGATA TAAGCGGAAA
    CTGAGAGAAG CCCAGTCTGA CCTGAACAAG ACACGTCTGC GCAGTGGCAG TGCCCTCCTG
    CAGTCTCAGT CCAGTACTGA GGATCCAAAG GATGAGCCTG CAGAGCTGAA GCAAGATCCT
    GAGGACGTAC CTGCCCAGTC CTCCGCATCA AAGGCATCTC AGGAGGAGGT CAATGAAATT
    AAGTCCAAAC GGGACGAGGA AGAACGAGAA CGAGAAAGGA GGGAGAAAGA GAGGGAGCGA
    GAAAGAGAGC GGGAGAAGGA AAAGGAGAGG GAACGAGAGA AGCAGAAACT GAAAGAGTCA
    GAAAAAGAAA GAGACTCTGC TAAGGATAAA GAGAAAGGGA AACATGACGA TGGAAGGAAA
    AAGGAAGCAG AAGTTATCAA ACAATTGAAG ATTGAACTCA AGAAGGCACA AGAGAGCCAA
    AAGGAGATGA AACTATTGCT AGATATGTAC CGCTCTGCCC CAAAGGAACA GAGAGACAAA
    GTTCAGCTGA TGGCAGCTGA GAAGAAGTCA AAGGCAGAGT TGGAAGATTT AAGGCAAAGA
    CTCAAGGATC TAGAGGATAA GGAGAAAAAG GAGAACAAGA AAATGGCTGA TGAGGATGCC
    TTGAGGAAGA TCCGGGCAGT AGAAGAGCAG ATAGAGTACC TGCAGAAGAA GCTGGCCATG
    GCCAAGCAGG AGGAAGAGGC ACTCCTCTCA GAGATGGATG TTACAGGCCA GGCCTTTGAA
    GACATGCAGG AGCAAAATAT CCGTTTAATG CAGCAGTTGC GGGAGAAGGA TGATGCAAAT
    TTCAAGCTCA TGTCTGAGCG TATCAAGTCC AATCAGATCC ACAAGTTGCT CAAAGAAGAG
    AAGGAGGAGC TGGCTGACCA AGTTTTGACT CTAAAGACTC AGGTTGATGC CCAGTTACAG
    GTAGTAAGGA AATTGGAAGA GAAGGAGCAT CTGTTACAAA GCAACATTGG CACAGGGGAG
    AAGGAACTGG GTCTTAGGAC ACAAGCCTTA GAGATGAATA AACGCAAGGC GATGGAAGCA
    GCCCAGCTGG CAGATGACCT CAAAGCGCAA CTGGAATTGG CTCAGAAGAA GCTACATGAT
    TTTCAGGATG AGATCGTGGA GAATAGTGTC ACCAAAGAAA AGGATATGTT TAATTTCAAA
    CGAGCTCAGG AGGACATCTC TAGACTCCGA AGGAAGCTGG AGACCACAAA GAAACCAGAC
    AATGTGCCCA AATGTGATGA GATCCTGATG GAGGAGATTA AGGATTACAA GGCACGCCTG
    ACCTGTCCAT GTTGCAATAT GCGTAAAAAG GATGCTGTAC TTACCAAGTG TTTTCATGTT
    TTCTGCTTTG AGTGTGTAAA GACACGCTAT GACACCCGTC AGCGCAAATG TCCCAAGTGT
    AATGCTGCGT TTGGTGCCAA TGATTTCCAC CGCATCTACA TTGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005626937.1
    CDS285..3212
    Translation

    Target ORF information:

    RefSeq Version XM_005626880.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ring finger protein 20, E3 ubiquitin protein ligase (RNF20), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005626880.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTCAGGAA TTGGAAATAA AAGAGCAGCT GGAGAGCCTG GCACATCCAT GCCTCCGGAG 
    AAGAAGACAG CCGTTGAAGA TTCAGGGACC ACAGTGGAGA CAATTAAGCT TGGAGGTGTC
    TCCTCCACGG AGGAGCTAGA CATTCGAACA CTGCAGACCA AAAATCGCAA GCTGGCAGAA
    ATGCTGGATC AACGGCAGGC CATCGAAGAT GAACTCCGTG AGCACATTGA AAAACTGGAG
    CGAAGACAGG CCACTGATGA TGCCTCACTG CTGATCGTCA ACCGGTACTG GAGTCAGTTT
    GATGAAAACA TCCGTATCAT CCTTAAACGG TACGATCTGG AGCAGGGTTT GGGAGATCTC
    CTCACAGAAA GGAAAGCCCT TGTCGTGCCC GAACCAGAGC CGGACTCTGA TAGCAATCAG
    GAACGGAAAG ATGACCGAGA GAGAGGGGAA GGGCAAGAGC CAGCTTTCTC TTTCCTTGCT
    ACTTTGGCCA GCAGTTCCAG TGAAGAAATG GAATCTCAGC TGCAGGAGCG AGTGGAGTCC
    TCCCGCCGAG CTGTGTCCCA GATCGTGACT GTCTATGATA AATTACAAGA GAAAGTGGAG
    CTGTTATCAT GCAAACTAAA TAGTGGAGAT AATCTGATAG TGGAAGAGGC GGTGCAGGAG
    CTGAATTCCT TCCTTGCACA GGAGAATATG AGGCTACAGG AATTGACAGA CCTCCTTCAG
    GAGAAGCATC GCACCATGTC TCAGGAGTTC TCCAAGTTAC AGAGTAAAGT GGAGACAGCC
    GAGTCGCGAG TGTCTGTCCT AGAGTCCATG ATCGATGACC TGCAGTGGGA TATTGACAAA
    ATTCGAAAGA GGGAACAACG ACTCAACCGA CACTTAGCAG AAGTTCTTGA ACGGGTGAAT
    TCGAAAGGTT ATAAGGTCTA TGGAGCCGGG AGCAGTCTCT ATGGTGGCAC AATCACTATC
    AATGCCCGGA AGTTTGAGGA AATGAATGCA GAGCTGGAGG AGAACAAAGA GTTGGCTCAG
    AACCGTCACT GTGAGCTGGA GAAACTTCGG CAAGACTTGG AGGAGGTCAC TACGCAAAAT
    GAAAAGCTGA AGGTGGAATT GCGGAGCGCG GTGGAGGAGG CAGTTAAGGA GACTCCGGAA
    TACCGCTGTA TGCAGTCACA GTTCTCCGTC CTGTACAATG AGAGCCTGCA GTTGAAAGCG
    CACTTGGATG AGGCTCGGAC TCTGCTTCAT GGCACCAGGG GGACCCACCA GCGCCAAGTT
    GAGCTTATTG AGCGAGATGA AGTTAGTCTT CATAAGAAGC TGAGGACTGA AGTGATCCAG
    CTGGAAGATA CTCTGGCCCA GGTCCGCAAG GAGTATGAGA TGCTGAGGAT AGAATTTGAG
    CAGACCCTTG CTGCCAATGA GCAAGCAGGC CCCATAAACC GGGAGATGCG TCACCTCATC
    AGTAGCCTCC AGAATCACAA TCACCAGCTG AAAGGGGAGG TCCTAAGATA TAAGCGGAAA
    CTGAGAGAAG CCCAGTCTGA CCTGAACAAG ACACGTCTGC GCAGTGGCAG TGCCCTCCTG
    CAGTCTCAGT CCAGTACTGA GGATCCAAAG GATGAGCCTG CAGAGCTGAA GCAAGATCCT
    GAGGACGTAC CTGCCCAGTC CTCCGCATCA AAGGCATCTC AGGAGGAGGT CAATGAAATT
    AAGTCCAAAC GGGACGAGGA AGAACGAGAA CGAGAAAGGA GGGAGAAAGA GAGGGAGCGA
    GAAAGAGAGC GGGAGAAGGA AAAGGAGAGG GAACGAGAGA AGCAGAAACT GAAAGAGTCA
    GAAAAAGAAA GAGACTCTGC TAAGGATAAA GAGAAAGGGA AACATGACGA TGGAAGGAAA
    AAGGAAGCAG AAGTTATCAA ACAATTGAAG ATTGAACTCA AGAAGGCACA AGAGAGCCAA
    AAGGAGATGA AACTATTGCT AGATATGTAC CGCTCTGCCC CAAAGGAACA GAGAGACAAA
    GTTCAGCTGA TGGCAGCTGA GAAGAAGTCA AAGGCAGAGT TGGAAGATTT AAGGCAAAGA
    CTCAAGGATC TAGAGGATAA GGAGAAAAAG GAGAACAAGA AAATGGCTGA TGAGGATGCC
    TTGAGGAAGA TCCGGGCAGT AGAAGAGCAG ATAGAGTACC TGCAGAAGAA GCTGGCCATG
    GCCAAGCAGG AGGAAGAGGC ACTCCTCTCA GAGATGGATG TTACAGGCCA GGCCTTTGAA
    GACATGCAGG AGCAAAATAT CCGTTTAATG CAGCAGTTGC GGGAGAAGGA TGATGCAAAT
    TTCAAGCTCA TGTCTGAGCG TATCAAGTCC AATCAGATCC ACAAGTTGCT CAAAGAAGAG
    AAGGAGGAGC TGGCTGACCA AGTTTTGACT CTAAAGACTC AGGTTGATGC CCAGTTACAG
    GTAGTAAGGA AATTGGAAGA GAAGGAGCAT CTGTTACAAA GCAACATTGG CACAGGGGAG
    AAGGAACTGG GTCTTAGGAC ACAAGCCTTA GAGATGAATA AACGCAAGGC GATGGAAGCA
    GCCCAGCTGG CAGATGACCT CAAAGCGCAA CTGGAATTGG CTCAGAAGAA GCTACATGAT
    TTTCAGGATG AGATCGTGGA GAATAGTGTC ACCAAAGAAA AGGATATGTT TAATTTCAAA
    CGAGCTCAGG AGGACATCTC TAGACTCCGA AGGAAGCTGG AGACCACAAA GAAACCAGAC
    AATGTGCCCA AATGTGATGA GATCCTGATG GAGGAGATTA AGGATTACAA GGCACGCCTG
    ACCTGTCCAT GTTGCAATAT GCGTAAAAAG GATGCTGTAC TTACCAAGTG TTTTCATGTT
    TTCTGCTTTG AGTGTGTAAA GACACGCTAT GACACCCGTC AGCGCAAATG TCCCAAGTGT
    AATGCTGCGT TTGGTGCCAA TGATTTCCAC CGCATCTACA TTGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe14708
    Clone ID Related Accession (Same CDS sequence) XM_005626880.2 , XM_022425660.1 , XM_005626880.3 , XM_532018.4 , XM_532018.5
    Accession Version XM_022425660.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2928bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase BRE1A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTCAGGAA TTGGAAATAA AAGAGCAGCT GGAGAGCCTG GCACATCCAT GCCTCCGGAG 
    AAGAAGACAG CCGTTGAAGA TTCAGGGACC ACAGTGGAGA CAATTAAGCT TGGAGGTGTC
    TCCTCCACGG AGGAGCTAGA CATTCGAACA CTGCAGACCA AAAATCGCAA GCTGGCAGAA
    ATGCTGGATC AACGGCAGGC CATCGAAGAT GAACTCCGTG AGCACATTGA AAAACTGGAG
    CGAAGACAGG CCACTGATGA TGCCTCACTG CTGATCGTCA ACCGGTACTG GAGTCAGTTT
    GATGAAAACA TCCGTATCAT CCTTAAACGG TACGATCTGG AGCAGGGTTT GGGAGATCTC
    CTCACAGAAA GGAAAGCCCT TGTCGTGCCC GAACCAGAGC CGGACTCTGA TAGCAATCAG
    GAACGGAAAG ATGACCGAGA GAGAGGGGAA GGGCAAGAGC CAGCTTTCTC TTTCCTTGCT
    ACTTTGGCCA GCAGTTCCAG TGAAGAAATG GAATCTCAGC TGCAGGAGCG AGTGGAGTCC
    TCCCGCCGAG CTGTGTCCCA GATCGTGACT GTCTATGATA AATTACAAGA GAAAGTGGAG
    CTGTTATCAT GCAAACTAAA TAGTGGAGAT AATCTGATAG TGGAAGAGGC GGTGCAGGAG
    CTGAATTCCT TCCTTGCACA GGAGAATATG AGGCTACAGG AATTGACAGA CCTCCTTCAG
    GAGAAGCATC GCACCATGTC TCAGGAGTTC TCCAAGTTAC AGAGTAAAGT GGAGACAGCC
    GAGTCGCGAG TGTCTGTCCT AGAGTCCATG ATCGATGACC TGCAGTGGGA TATTGACAAA
    ATTCGAAAGA GGGAACAACG ACTCAACCGA CACTTAGCAG AAGTTCTTGA ACGGGTGAAT
    TCGAAAGGTT ATAAGGTCTA TGGAGCCGGG AGCAGTCTCT ATGGTGGCAC AATCACTATC
    AATGCCCGGA AGTTTGAGGA AATGAATGCA GAGCTGGAGG AGAACAAAGA GTTGGCTCAG
    AACCGTCACT GTGAGCTGGA GAAACTTCGG CAAGACTTGG AGGAGGTCAC TACGCAAAAT
    GAAAAGCTGA AGGTGGAATT GCGGAGCGCG GTGGAGGAGG CAGTTAAGGA GACTCCGGAA
    TACCGCTGTA TGCAGTCACA GTTCTCCGTC CTGTACAATG AGAGCCTGCA GTTGAAAGCG
    CACTTGGATG AGGCTCGGAC TCTGCTTCAT GGCACCAGGG GGACCCACCA GCGCCAAGTT
    GAGCTTATTG AGCGAGATGA AGTTAGTCTT CATAAGAAGC TGAGGACTGA AGTGATCCAG
    CTGGAAGATA CTCTGGCCCA GGTCCGCAAG GAGTATGAGA TGCTGAGGAT AGAATTTGAG
    CAGACCCTTG CTGCCAATGA GCAAGCAGGC CCCATAAACC GGGAGATGCG TCACCTCATC
    AGTAGCCTCC AGAATCACAA TCACCAGCTG AAAGGGGAGG TCCTAAGATA TAAGCGGAAA
    CTGAGAGAAG CCCAGTCTGA CCTGAACAAG ACACGTCTGC GCAGTGGCAG TGCCCTCCTG
    CAGTCTCAGT CCAGTACTGA GGATCCAAAG GATGAGCCTG CAGAGCTGAA GCAAGATCCT
    GAGGACGTAC CTGCCCAGTC CTCCGCATCA AAGGCATCTC AGGAGGAGGT CAATGAAATT
    AAGTCCAAAC GGGACGAGGA AGAACGAGAA CGAGAAAGGA GGGAGAAAGA GAGGGAGCGA
    GAAAGAGAGC GGGAGAAGGA AAAGGAGAGG GAACGAGAGA AGCAGAAACT GAAAGAGTCA
    GAAAAAGAAA GAGACTCTGC TAAGGATAAA GAGAAAGGGA AACATGACGA TGGAAGGAAA
    AAGGAAGCAG AAGTTATCAA ACAATTGAAG ATTGAACTCA AGAAGGCACA AGAGAGCCAA
    AAGGAGATGA AACTATTGCT AGATATGTAC CGCTCTGCCC CAAAGGAACA GAGAGACAAA
    GTTCAGCTGA TGGCAGCTGA GAAGAAGTCA AAGGCAGAGT TGGAAGATTT AAGGCAAAGA
    CTCAAGGATC TAGAGGATAA GGAGAAAAAG GAGAACAAGA AAATGGCTGA TGAGGATGCC
    TTGAGGAAGA TCCGGGCAGT AGAAGAGCAG ATAGAGTACC TGCAGAAGAA GCTGGCCATG
    GCCAAGCAGG AGGAAGAGGC ACTCCTCTCA GAGATGGATG TTACAGGCCA GGCCTTTGAA
    GACATGCAGG AGCAAAATAT CCGTTTAATG CAGCAGTTGC GGGAGAAGGA TGATGCAAAT
    TTCAAGCTCA TGTCTGAGCG TATCAAGTCC AATCAGATCC ACAAGTTGCT CAAAGAAGAG
    AAGGAGGAGC TGGCTGACCA AGTTTTGACT CTAAAGACTC AGGTTGATGC CCAGTTACAG
    GTAGTAAGGA AATTGGAAGA GAAGGAGCAT CTGTTACAAA GCAACATTGG CACAGGGGAG
    AAGGAACTGG GTCTTAGGAC ACAAGCCTTA GAGATGAATA AACGCAAGGC GATGGAAGCA
    GCCCAGCTGG CAGATGACCT CAAAGCGCAA CTGGAATTGG CTCAGAAGAA GCTACATGAT
    TTTCAGGATG AGATCGTGGA GAATAGTGTC ACCAAAGAAA AGGATATGTT TAATTTCAAA
    CGAGCTCAGG AGGACATCTC TAGACTCCGA AGGAAGCTGG AGACCACAAA GAAACCAGAC
    AATGTGCCCA AATGTGATGA GATCCTGATG GAGGAGATTA AGGATTACAA GGCACGCCTG
    ACCTGTCCAT GTTGCAATAT GCGTAAAAAG GATGCTGTAC TTACCAAGTG TTTTCATGTT
    TTCTGCTTTG AGTGTGTAAA GACACGCTAT GACACCCGTC AGCGCAAATG TCCCAAGTGT
    AATGCTGCGT TTGGTGCCAA TGATTTCCAC CGCATCTACA TTGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022281368.1
    CDS152..3079
    Translation

    Target ORF information:

    RefSeq Version XM_022425660.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ring finger protein 20 (RNF20), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022425660.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTCAGGAA TTGGAAATAA AAGAGCAGCT GGAGAGCCTG GCACATCCAT GCCTCCGGAG 
    AAGAAGACAG CCGTTGAAGA TTCAGGGACC ACAGTGGAGA CAATTAAGCT TGGAGGTGTC
    TCCTCCACGG AGGAGCTAGA CATTCGAACA CTGCAGACCA AAAATCGCAA GCTGGCAGAA
    ATGCTGGATC AACGGCAGGC CATCGAAGAT GAACTCCGTG AGCACATTGA AAAACTGGAG
    CGAAGACAGG CCACTGATGA TGCCTCACTG CTGATCGTCA ACCGGTACTG GAGTCAGTTT
    GATGAAAACA TCCGTATCAT CCTTAAACGG TACGATCTGG AGCAGGGTTT GGGAGATCTC
    CTCACAGAAA GGAAAGCCCT TGTCGTGCCC GAACCAGAGC CGGACTCTGA TAGCAATCAG
    GAACGGAAAG ATGACCGAGA GAGAGGGGAA GGGCAAGAGC CAGCTTTCTC TTTCCTTGCT
    ACTTTGGCCA GCAGTTCCAG TGAAGAAATG GAATCTCAGC TGCAGGAGCG AGTGGAGTCC
    TCCCGCCGAG CTGTGTCCCA GATCGTGACT GTCTATGATA AATTACAAGA GAAAGTGGAG
    CTGTTATCAT GCAAACTAAA TAGTGGAGAT AATCTGATAG TGGAAGAGGC GGTGCAGGAG
    CTGAATTCCT TCCTTGCACA GGAGAATATG AGGCTACAGG AATTGACAGA CCTCCTTCAG
    GAGAAGCATC GCACCATGTC TCAGGAGTTC TCCAAGTTAC AGAGTAAAGT GGAGACAGCC
    GAGTCGCGAG TGTCTGTCCT AGAGTCCATG ATCGATGACC TGCAGTGGGA TATTGACAAA
    ATTCGAAAGA GGGAACAACG ACTCAACCGA CACTTAGCAG AAGTTCTTGA ACGGGTGAAT
    TCGAAAGGTT ATAAGGTCTA TGGAGCCGGG AGCAGTCTCT ATGGTGGCAC AATCACTATC
    AATGCCCGGA AGTTTGAGGA AATGAATGCA GAGCTGGAGG AGAACAAAGA GTTGGCTCAG
    AACCGTCACT GTGAGCTGGA GAAACTTCGG CAAGACTTGG AGGAGGTCAC TACGCAAAAT
    GAAAAGCTGA AGGTGGAATT GCGGAGCGCG GTGGAGGAGG CAGTTAAGGA GACTCCGGAA
    TACCGCTGTA TGCAGTCACA GTTCTCCGTC CTGTACAATG AGAGCCTGCA GTTGAAAGCG
    CACTTGGATG AGGCTCGGAC TCTGCTTCAT GGCACCAGGG GGACCCACCA GCGCCAAGTT
    GAGCTTATTG AGCGAGATGA AGTTAGTCTT CATAAGAAGC TGAGGACTGA AGTGATCCAG
    CTGGAAGATA CTCTGGCCCA GGTCCGCAAG GAGTATGAGA TGCTGAGGAT AGAATTTGAG
    CAGACCCTTG CTGCCAATGA GCAAGCAGGC CCCATAAACC GGGAGATGCG TCACCTCATC
    AGTAGCCTCC AGAATCACAA TCACCAGCTG AAAGGGGAGG TCCTAAGATA TAAGCGGAAA
    CTGAGAGAAG CCCAGTCTGA CCTGAACAAG ACACGTCTGC GCAGTGGCAG TGCCCTCCTG
    CAGTCTCAGT CCAGTACTGA GGATCCAAAG GATGAGCCTG CAGAGCTGAA GCAAGATCCT
    GAGGACGTAC CTGCCCAGTC CTCCGCATCA AAGGCATCTC AGGAGGAGGT CAATGAAATT
    AAGTCCAAAC GGGACGAGGA AGAACGAGAA CGAGAAAGGA GGGAGAAAGA GAGGGAGCGA
    GAAAGAGAGC GGGAGAAGGA AAAGGAGAGG GAACGAGAGA AGCAGAAACT GAAAGAGTCA
    GAAAAAGAAA GAGACTCTGC TAAGGATAAA GAGAAAGGGA AACATGACGA TGGAAGGAAA
    AAGGAAGCAG AAGTTATCAA ACAATTGAAG ATTGAACTCA AGAAGGCACA AGAGAGCCAA
    AAGGAGATGA AACTATTGCT AGATATGTAC CGCTCTGCCC CAAAGGAACA GAGAGACAAA
    GTTCAGCTGA TGGCAGCTGA GAAGAAGTCA AAGGCAGAGT TGGAAGATTT AAGGCAAAGA
    CTCAAGGATC TAGAGGATAA GGAGAAAAAG GAGAACAAGA AAATGGCTGA TGAGGATGCC
    TTGAGGAAGA TCCGGGCAGT AGAAGAGCAG ATAGAGTACC TGCAGAAGAA GCTGGCCATG
    GCCAAGCAGG AGGAAGAGGC ACTCCTCTCA GAGATGGATG TTACAGGCCA GGCCTTTGAA
    GACATGCAGG AGCAAAATAT CCGTTTAATG CAGCAGTTGC GGGAGAAGGA TGATGCAAAT
    TTCAAGCTCA TGTCTGAGCG TATCAAGTCC AATCAGATCC ACAAGTTGCT CAAAGAAGAG
    AAGGAGGAGC TGGCTGACCA AGTTTTGACT CTAAAGACTC AGGTTGATGC CCAGTTACAG
    GTAGTAAGGA AATTGGAAGA GAAGGAGCAT CTGTTACAAA GCAACATTGG CACAGGGGAG
    AAGGAACTGG GTCTTAGGAC ACAAGCCTTA GAGATGAATA AACGCAAGGC GATGGAAGCA
    GCCCAGCTGG CAGATGACCT CAAAGCGCAA CTGGAATTGG CTCAGAAGAA GCTACATGAT
    TTTCAGGATG AGATCGTGGA GAATAGTGTC ACCAAAGAAA AGGATATGTT TAATTTCAAA
    CGAGCTCAGG AGGACATCTC TAGACTCCGA AGGAAGCTGG AGACCACAAA GAAACCAGAC
    AATGTGCCCA AATGTGATGA GATCCTGATG GAGGAGATTA AGGATTACAA GGCACGCCTG
    ACCTGTCCAT GTTGCAATAT GCGTAAAAAG GATGCTGTAC TTACCAAGTG TTTTCATGTT
    TTCTGCTTTG AGTGTGTAAA GACACGCTAT GACACCCGTC AGCGCAAATG TCCCAAGTGT
    AATGCTGCGT TTGGTGCCAA TGATTTCCAC CGCATCTACA TTGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe14708
    Clone ID Related Accession (Same CDS sequence) XM_005626880.2 , XM_022425660.1 , XM_005626880.3 , XM_532018.4 , XM_532018.5
    Accession Version XM_005626880.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2928bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase BRE1A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005626880.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTCAGGAA TTGGAAATAA AAGAGCAGCT GGAGAGCCTG GCACATCCAT GCCTCCGGAG 
    AAGAAGACAG CCGTTGAAGA TTCAGGGACC ACAGTGGAGA CAATTAAGCT TGGAGGTGTC
    TCCTCCACGG AGGAGCTAGA CATTCGAACA CTGCAGACCA AAAATCGCAA GCTGGCAGAA
    ATGCTGGATC AACGGCAGGC CATCGAAGAT GAACTCCGTG AGCACATTGA AAAACTGGAG
    CGAAGACAGG CCACTGATGA TGCCTCACTG CTGATCGTCA ACCGGTACTG GAGTCAGTTT
    GATGAAAACA TCCGTATCAT CCTTAAACGG TACGATCTGG AGCAGGGTTT GGGAGATCTC
    CTCACAGAAA GGAAAGCCCT TGTCGTGCCC GAACCAGAGC CGGACTCTGA TAGCAATCAG
    GAACGGAAAG ATGACCGAGA GAGAGGGGAA GGGCAAGAGC CAGCTTTCTC TTTCCTTGCT
    ACTTTGGCCA GCAGTTCCAG TGAAGAAATG GAATCTCAGC TGCAGGAGCG AGTGGAGTCC
    TCCCGCCGAG CTGTGTCCCA GATCGTGACT GTCTATGATA AATTACAAGA GAAAGTGGAG
    CTGTTATCAT GCAAACTAAA TAGTGGAGAT AATCTGATAG TGGAAGAGGC GGTGCAGGAG
    CTGAATTCCT TCCTTGCACA GGAGAATATG AGGCTACAGG AATTGACAGA CCTCCTTCAG
    GAGAAGCATC GCACCATGTC TCAGGAGTTC TCCAAGTTAC AGAGTAAAGT GGAGACAGCC
    GAGTCGCGAG TGTCTGTCCT AGAGTCCATG ATCGATGACC TGCAGTGGGA TATTGACAAA
    ATTCGAAAGA GGGAACAACG ACTCAACCGA CACTTAGCAG AAGTTCTTGA ACGGGTGAAT
    TCGAAAGGTT ATAAGGTCTA TGGAGCCGGG AGCAGTCTCT ATGGTGGCAC AATCACTATC
    AATGCCCGGA AGTTTGAGGA AATGAATGCA GAGCTGGAGG AGAACAAAGA GTTGGCTCAG
    AACCGTCACT GTGAGCTGGA GAAACTTCGG CAAGACTTGG AGGAGGTCAC TACGCAAAAT
    GAAAAGCTGA AGGTGGAATT GCGGAGCGCG GTGGAGGAGG CAGTTAAGGA GACTCCGGAA
    TACCGCTGTA TGCAGTCACA GTTCTCCGTC CTGTACAATG AGAGCCTGCA GTTGAAAGCG
    CACTTGGATG AGGCTCGGAC TCTGCTTCAT GGCACCAGGG GGACCCACCA GCGCCAAGTT
    GAGCTTATTG AGCGAGATGA AGTTAGTCTT CATAAGAAGC TGAGGACTGA AGTGATCCAG
    CTGGAAGATA CTCTGGCCCA GGTCCGCAAG GAGTATGAGA TGCTGAGGAT AGAATTTGAG
    CAGACCCTTG CTGCCAATGA GCAAGCAGGC CCCATAAACC GGGAGATGCG TCACCTCATC
    AGTAGCCTCC AGAATCACAA TCACCAGCTG AAAGGGGAGG TCCTAAGATA TAAGCGGAAA
    CTGAGAGAAG CCCAGTCTGA CCTGAACAAG ACACGTCTGC GCAGTGGCAG TGCCCTCCTG
    CAGTCTCAGT CCAGTACTGA GGATCCAAAG GATGAGCCTG CAGAGCTGAA GCAAGATCCT
    GAGGACGTAC CTGCCCAGTC CTCCGCATCA AAGGCATCTC AGGAGGAGGT CAATGAAATT
    AAGTCCAAAC GGGACGAGGA AGAACGAGAA CGAGAAAGGA GGGAGAAAGA GAGGGAGCGA
    GAAAGAGAGC GGGAGAAGGA AAAGGAGAGG GAACGAGAGA AGCAGAAACT GAAAGAGTCA
    GAAAAAGAAA GAGACTCTGC TAAGGATAAA GAGAAAGGGA AACATGACGA TGGAAGGAAA
    AAGGAAGCAG AAGTTATCAA ACAATTGAAG ATTGAACTCA AGAAGGCACA AGAGAGCCAA
    AAGGAGATGA AACTATTGCT AGATATGTAC CGCTCTGCCC CAAAGGAACA GAGAGACAAA
    GTTCAGCTGA TGGCAGCTGA GAAGAAGTCA AAGGCAGAGT TGGAAGATTT AAGGCAAAGA
    CTCAAGGATC TAGAGGATAA GGAGAAAAAG GAGAACAAGA AAATGGCTGA TGAGGATGCC
    TTGAGGAAGA TCCGGGCAGT AGAAGAGCAG ATAGAGTACC TGCAGAAGAA GCTGGCCATG
    GCCAAGCAGG AGGAAGAGGC ACTCCTCTCA GAGATGGATG TTACAGGCCA GGCCTTTGAA
    GACATGCAGG AGCAAAATAT CCGTTTAATG CAGCAGTTGC GGGAGAAGGA TGATGCAAAT
    TTCAAGCTCA TGTCTGAGCG TATCAAGTCC AATCAGATCC ACAAGTTGCT CAAAGAAGAG
    AAGGAGGAGC TGGCTGACCA AGTTTTGACT CTAAAGACTC AGGTTGATGC CCAGTTACAG
    GTAGTAAGGA AATTGGAAGA GAAGGAGCAT CTGTTACAAA GCAACATTGG CACAGGGGAG
    AAGGAACTGG GTCTTAGGAC ACAAGCCTTA GAGATGAATA AACGCAAGGC GATGGAAGCA
    GCCCAGCTGG CAGATGACCT CAAAGCGCAA CTGGAATTGG CTCAGAAGAA GCTACATGAT
    TTTCAGGATG AGATCGTGGA GAATAGTGTC ACCAAAGAAA AGGATATGTT TAATTTCAAA
    CGAGCTCAGG AGGACATCTC TAGACTCCGA AGGAAGCTGG AGACCACAAA GAAACCAGAC
    AATGTGCCCA AATGTGATGA GATCCTGATG GAGGAGATTA AGGATTACAA GGCACGCCTG
    ACCTGTCCAT GTTGCAATAT GCGTAAAAAG GATGCTGTAC TTACCAAGTG TTTTCATGTT
    TTCTGCTTTG AGTGTGTAAA GACACGCTAT GACACCCGTC AGCGCAAATG TCCCAAGTGT
    AATGCTGCGT TTGGTGCCAA TGATTTCCAC CGCATCTACA TTGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005626937.1
    CDS178..3105
    Translation

    Target ORF information:

    RefSeq Version XM_005626880.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ring finger protein 20 (RNF20), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005626880.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTCAGGAA TTGGAAATAA AAGAGCAGCT GGAGAGCCTG GCACATCCAT GCCTCCGGAG 
    AAGAAGACAG CCGTTGAAGA TTCAGGGACC ACAGTGGAGA CAATTAAGCT TGGAGGTGTC
    TCCTCCACGG AGGAGCTAGA CATTCGAACA CTGCAGACCA AAAATCGCAA GCTGGCAGAA
    ATGCTGGATC AACGGCAGGC CATCGAAGAT GAACTCCGTG AGCACATTGA AAAACTGGAG
    CGAAGACAGG CCACTGATGA TGCCTCACTG CTGATCGTCA ACCGGTACTG GAGTCAGTTT
    GATGAAAACA TCCGTATCAT CCTTAAACGG TACGATCTGG AGCAGGGTTT GGGAGATCTC
    CTCACAGAAA GGAAAGCCCT TGTCGTGCCC GAACCAGAGC CGGACTCTGA TAGCAATCAG
    GAACGGAAAG ATGACCGAGA GAGAGGGGAA GGGCAAGAGC CAGCTTTCTC TTTCCTTGCT
    ACTTTGGCCA GCAGTTCCAG TGAAGAAATG GAATCTCAGC TGCAGGAGCG AGTGGAGTCC
    TCCCGCCGAG CTGTGTCCCA GATCGTGACT GTCTATGATA AATTACAAGA GAAAGTGGAG
    CTGTTATCAT GCAAACTAAA TAGTGGAGAT AATCTGATAG TGGAAGAGGC GGTGCAGGAG
    CTGAATTCCT TCCTTGCACA GGAGAATATG AGGCTACAGG AATTGACAGA CCTCCTTCAG
    GAGAAGCATC GCACCATGTC TCAGGAGTTC TCCAAGTTAC AGAGTAAAGT GGAGACAGCC
    GAGTCGCGAG TGTCTGTCCT AGAGTCCATG ATCGATGACC TGCAGTGGGA TATTGACAAA
    ATTCGAAAGA GGGAACAACG ACTCAACCGA CACTTAGCAG AAGTTCTTGA ACGGGTGAAT
    TCGAAAGGTT ATAAGGTCTA TGGAGCCGGG AGCAGTCTCT ATGGTGGCAC AATCACTATC
    AATGCCCGGA AGTTTGAGGA AATGAATGCA GAGCTGGAGG AGAACAAAGA GTTGGCTCAG
    AACCGTCACT GTGAGCTGGA GAAACTTCGG CAAGACTTGG AGGAGGTCAC TACGCAAAAT
    GAAAAGCTGA AGGTGGAATT GCGGAGCGCG GTGGAGGAGG CAGTTAAGGA GACTCCGGAA
    TACCGCTGTA TGCAGTCACA GTTCTCCGTC CTGTACAATG AGAGCCTGCA GTTGAAAGCG
    CACTTGGATG AGGCTCGGAC TCTGCTTCAT GGCACCAGGG GGACCCACCA GCGCCAAGTT
    GAGCTTATTG AGCGAGATGA AGTTAGTCTT CATAAGAAGC TGAGGACTGA AGTGATCCAG
    CTGGAAGATA CTCTGGCCCA GGTCCGCAAG GAGTATGAGA TGCTGAGGAT AGAATTTGAG
    CAGACCCTTG CTGCCAATGA GCAAGCAGGC CCCATAAACC GGGAGATGCG TCACCTCATC
    AGTAGCCTCC AGAATCACAA TCACCAGCTG AAAGGGGAGG TCCTAAGATA TAAGCGGAAA
    CTGAGAGAAG CCCAGTCTGA CCTGAACAAG ACACGTCTGC GCAGTGGCAG TGCCCTCCTG
    CAGTCTCAGT CCAGTACTGA GGATCCAAAG GATGAGCCTG CAGAGCTGAA GCAAGATCCT
    GAGGACGTAC CTGCCCAGTC CTCCGCATCA AAGGCATCTC AGGAGGAGGT CAATGAAATT
    AAGTCCAAAC GGGACGAGGA AGAACGAGAA CGAGAAAGGA GGGAGAAAGA GAGGGAGCGA
    GAAAGAGAGC GGGAGAAGGA AAAGGAGAGG GAACGAGAGA AGCAGAAACT GAAAGAGTCA
    GAAAAAGAAA GAGACTCTGC TAAGGATAAA GAGAAAGGGA AACATGACGA TGGAAGGAAA
    AAGGAAGCAG AAGTTATCAA ACAATTGAAG ATTGAACTCA AGAAGGCACA AGAGAGCCAA
    AAGGAGATGA AACTATTGCT AGATATGTAC CGCTCTGCCC CAAAGGAACA GAGAGACAAA
    GTTCAGCTGA TGGCAGCTGA GAAGAAGTCA AAGGCAGAGT TGGAAGATTT AAGGCAAAGA
    CTCAAGGATC TAGAGGATAA GGAGAAAAAG GAGAACAAGA AAATGGCTGA TGAGGATGCC
    TTGAGGAAGA TCCGGGCAGT AGAAGAGCAG ATAGAGTACC TGCAGAAGAA GCTGGCCATG
    GCCAAGCAGG AGGAAGAGGC ACTCCTCTCA GAGATGGATG TTACAGGCCA GGCCTTTGAA
    GACATGCAGG AGCAAAATAT CCGTTTAATG CAGCAGTTGC GGGAGAAGGA TGATGCAAAT
    TTCAAGCTCA TGTCTGAGCG TATCAAGTCC AATCAGATCC ACAAGTTGCT CAAAGAAGAG
    AAGGAGGAGC TGGCTGACCA AGTTTTGACT CTAAAGACTC AGGTTGATGC CCAGTTACAG
    GTAGTAAGGA AATTGGAAGA GAAGGAGCAT CTGTTACAAA GCAACATTGG CACAGGGGAG
    AAGGAACTGG GTCTTAGGAC ACAAGCCTTA GAGATGAATA AACGCAAGGC GATGGAAGCA
    GCCCAGCTGG CAGATGACCT CAAAGCGCAA CTGGAATTGG CTCAGAAGAA GCTACATGAT
    TTTCAGGATG AGATCGTGGA GAATAGTGTC ACCAAAGAAA AGGATATGTT TAATTTCAAA
    CGAGCTCAGG AGGACATCTC TAGACTCCGA AGGAAGCTGG AGACCACAAA GAAACCAGAC
    AATGTGCCCA AATGTGATGA GATCCTGATG GAGGAGATTA AGGATTACAA GGCACGCCTG
    ACCTGTCCAT GTTGCAATAT GCGTAAAAAG GATGCTGTAC TTACCAAGTG TTTTCATGTT
    TTCTGCTTTG AGTGTGTAAA GACACGCTAT GACACCCGTC AGCGCAAATG TCCCAAGTGT
    AATGCTGCGT TTGGTGCCAA TGATTTCCAC CGCATCTACA TTGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe14708
    Clone ID Related Accession (Same CDS sequence) XM_005626880.2 , XM_022425660.1 , XM_005626880.3 , XM_532018.4 , XM_532018.5
    Accession Version XM_532018.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2928bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase BRE1A
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2013 this sequence version replaced XM_532018.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTCAGGAA TTGGAAATAA AAGAGCAGCT GGAGAGCCTG GCACATCCAT GCCTCCGGAG 
    AAGAAGACAG CCGTTGAAGA TTCAGGGACC ACAGTGGAGA CAATTAAGCT TGGAGGTGTC
    TCCTCCACGG AGGAGCTAGA CATTCGAACA CTGCAGACCA AAAATCGCAA GCTGGCAGAA
    ATGCTGGATC AACGGCAGGC CATCGAAGAT GAACTCCGTG AGCACATTGA AAAACTGGAG
    CGAAGACAGG CCACTGATGA TGCCTCACTG CTGATCGTCA ACCGGTACTG GAGTCAGTTT
    GATGAAAACA TCCGTATCAT CCTTAAACGG TACGATCTGG AGCAGGGTTT GGGAGATCTC
    CTCACAGAAA GGAAAGCCCT TGTCGTGCCC GAACCAGAGC CGGACTCTGA TAGCAATCAG
    GAACGGAAAG ATGACCGAGA GAGAGGGGAA GGGCAAGAGC CAGCTTTCTC TTTCCTTGCT
    ACTTTGGCCA GCAGTTCCAG TGAAGAAATG GAATCTCAGC TGCAGGAGCG AGTGGAGTCC
    TCCCGCCGAG CTGTGTCCCA GATCGTGACT GTCTATGATA AATTACAAGA GAAAGTGGAG
    CTGTTATCAT GCAAACTAAA TAGTGGAGAT AATCTGATAG TGGAAGAGGC GGTGCAGGAG
    CTGAATTCCT TCCTTGCACA GGAGAATATG AGGCTACAGG AATTGACAGA CCTCCTTCAG
    GAGAAGCATC GCACCATGTC TCAGGAGTTC TCCAAGTTAC AGAGTAAAGT GGAGACAGCC
    GAGTCGCGAG TGTCTGTCCT AGAGTCCATG ATCGATGACC TGCAGTGGGA TATTGACAAA
    ATTCGAAAGA GGGAACAACG ACTCAACCGA CACTTAGCAG AAGTTCTTGA ACGGGTGAAT
    TCGAAAGGTT ATAAGGTCTA TGGAGCCGGG AGCAGTCTCT ATGGTGGCAC AATCACTATC
    AATGCCCGGA AGTTTGAGGA AATGAATGCA GAGCTGGAGG AGAACAAAGA GTTGGCTCAG
    AACCGTCACT GTGAGCTGGA GAAACTTCGG CAAGACTTGG AGGAGGTCAC TACGCAAAAT
    GAAAAGCTGA AGGTGGAATT GCGGAGCGCG GTGGAGGAGG CAGTTAAGGA GACTCCGGAA
    TACCGCTGTA TGCAGTCACA GTTCTCCGTC CTGTACAATG AGAGCCTGCA GTTGAAAGCG
    CACTTGGATG AGGCTCGGAC TCTGCTTCAT GGCACCAGGG GGACCCACCA GCGCCAAGTT
    GAGCTTATTG AGCGAGATGA AGTTAGTCTT CATAAGAAGC TGAGGACTGA AGTGATCCAG
    CTGGAAGATA CTCTGGCCCA GGTCCGCAAG GAGTATGAGA TGCTGAGGAT AGAATTTGAG
    CAGACCCTTG CTGCCAATGA GCAAGCAGGC CCCATAAACC GGGAGATGCG TCACCTCATC
    AGTAGCCTCC AGAATCACAA TCACCAGCTG AAAGGGGAGG TCCTAAGATA TAAGCGGAAA
    CTGAGAGAAG CCCAGTCTGA CCTGAACAAG ACACGTCTGC GCAGTGGCAG TGCCCTCCTG
    CAGTCTCAGT CCAGTACTGA GGATCCAAAG GATGAGCCTG CAGAGCTGAA GCAAGATCCT
    GAGGACGTAC CTGCCCAGTC CTCCGCATCA AAGGCATCTC AGGAGGAGGT CAATGAAATT
    AAGTCCAAAC GGGACGAGGA AGAACGAGAA CGAGAAAGGA GGGAGAAAGA GAGGGAGCGA
    GAAAGAGAGC GGGAGAAGGA AAAGGAGAGG GAACGAGAGA AGCAGAAACT GAAAGAGTCA
    GAAAAAGAAA GAGACTCTGC TAAGGATAAA GAGAAAGGGA AACATGACGA TGGAAGGAAA
    AAGGAAGCAG AAGTTATCAA ACAATTGAAG ATTGAACTCA AGAAGGCACA AGAGAGCCAA
    AAGGAGATGA AACTATTGCT AGATATGTAC CGCTCTGCCC CAAAGGAACA GAGAGACAAA
    GTTCAGCTGA TGGCAGCTGA GAAGAAGTCA AAGGCAGAGT TGGAAGATTT AAGGCAAAGA
    CTCAAGGATC TAGAGGATAA GGAGAAAAAG GAGAACAAGA AAATGGCTGA TGAGGATGCC
    TTGAGGAAGA TCCGGGCAGT AGAAGAGCAG ATAGAGTACC TGCAGAAGAA GCTGGCCATG
    GCCAAGCAGG AGGAAGAGGC ACTCCTCTCA GAGATGGATG TTACAGGCCA GGCCTTTGAA
    GACATGCAGG AGCAAAATAT CCGTTTAATG CAGCAGTTGC GGGAGAAGGA TGATGCAAAT
    TTCAAGCTCA TGTCTGAGCG TATCAAGTCC AATCAGATCC ACAAGTTGCT CAAAGAAGAG
    AAGGAGGAGC TGGCTGACCA AGTTTTGACT CTAAAGACTC AGGTTGATGC CCAGTTACAG
    GTAGTAAGGA AATTGGAAGA GAAGGAGCAT CTGTTACAAA GCAACATTGG CACAGGGGAG
    AAGGAACTGG GTCTTAGGAC ACAAGCCTTA GAGATGAATA AACGCAAGGC GATGGAAGCA
    GCCCAGCTGG CAGATGACCT CAAAGCGCAA CTGGAATTGG CTCAGAAGAA GCTACATGAT
    TTTCAGGATG AGATCGTGGA GAATAGTGTC ACCAAAGAAA AGGATATGTT TAATTTCAAA
    CGAGCTCAGG AGGACATCTC TAGACTCCGA AGGAAGCTGG AGACCACAAA GAAACCAGAC
    AATGTGCCCA AATGTGATGA GATCCTGATG GAGGAGATTA AGGATTACAA GGCACGCCTG
    ACCTGTCCAT GTTGCAATAT GCGTAAAAAG GATGCTGTAC TTACCAAGTG TTTTCATGTT
    TTCTGCTTTG AGTGTGTAAA GACACGCTAT GACACCCGTC AGCGCAAATG TCCCAAGTGT
    AATGCTGCGT TTGGTGCCAA TGATTTCCAC CGCATCTACA TTGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_532018.3
    CDS183..3110
    Translation

    Target ORF information:

    RefSeq Version XM_532018.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ring finger protein 20, E3 ubiquitin protein ligase (RNF20), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_532018.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTCAGGAA TTGGAAATAA AAGAGCAGCT GGAGAGCCTG GCACATCCAT GCCTCCGGAG 
    AAGAAGACAG CCGTTGAAGA TTCAGGGACC ACAGTGGAGA CAATTAAGCT TGGAGGTGTC
    TCCTCCACGG AGGAGCTAGA CATTCGAACA CTGCAGACCA AAAATCGCAA GCTGGCAGAA
    ATGCTGGATC AACGGCAGGC CATCGAAGAT GAACTCCGTG AGCACATTGA AAAACTGGAG
    CGAAGACAGG CCACTGATGA TGCCTCACTG CTGATCGTCA ACCGGTACTG GAGTCAGTTT
    GATGAAAACA TCCGTATCAT CCTTAAACGG TACGATCTGG AGCAGGGTTT GGGAGATCTC
    CTCACAGAAA GGAAAGCCCT TGTCGTGCCC GAACCAGAGC CGGACTCTGA TAGCAATCAG
    GAACGGAAAG ATGACCGAGA GAGAGGGGAA GGGCAAGAGC CAGCTTTCTC TTTCCTTGCT
    ACTTTGGCCA GCAGTTCCAG TGAAGAAATG GAATCTCAGC TGCAGGAGCG AGTGGAGTCC
    TCCCGCCGAG CTGTGTCCCA GATCGTGACT GTCTATGATA AATTACAAGA GAAAGTGGAG
    CTGTTATCAT GCAAACTAAA TAGTGGAGAT AATCTGATAG TGGAAGAGGC GGTGCAGGAG
    CTGAATTCCT TCCTTGCACA GGAGAATATG AGGCTACAGG AATTGACAGA CCTCCTTCAG
    GAGAAGCATC GCACCATGTC TCAGGAGTTC TCCAAGTTAC AGAGTAAAGT GGAGACAGCC
    GAGTCGCGAG TGTCTGTCCT AGAGTCCATG ATCGATGACC TGCAGTGGGA TATTGACAAA
    ATTCGAAAGA GGGAACAACG ACTCAACCGA CACTTAGCAG AAGTTCTTGA ACGGGTGAAT
    TCGAAAGGTT ATAAGGTCTA TGGAGCCGGG AGCAGTCTCT ATGGTGGCAC AATCACTATC
    AATGCCCGGA AGTTTGAGGA AATGAATGCA GAGCTGGAGG AGAACAAAGA GTTGGCTCAG
    AACCGTCACT GTGAGCTGGA GAAACTTCGG CAAGACTTGG AGGAGGTCAC TACGCAAAAT
    GAAAAGCTGA AGGTGGAATT GCGGAGCGCG GTGGAGGAGG CAGTTAAGGA GACTCCGGAA
    TACCGCTGTA TGCAGTCACA GTTCTCCGTC CTGTACAATG AGAGCCTGCA GTTGAAAGCG
    CACTTGGATG AGGCTCGGAC TCTGCTTCAT GGCACCAGGG GGACCCACCA GCGCCAAGTT
    GAGCTTATTG AGCGAGATGA AGTTAGTCTT CATAAGAAGC TGAGGACTGA AGTGATCCAG
    CTGGAAGATA CTCTGGCCCA GGTCCGCAAG GAGTATGAGA TGCTGAGGAT AGAATTTGAG
    CAGACCCTTG CTGCCAATGA GCAAGCAGGC CCCATAAACC GGGAGATGCG TCACCTCATC
    AGTAGCCTCC AGAATCACAA TCACCAGCTG AAAGGGGAGG TCCTAAGATA TAAGCGGAAA
    CTGAGAGAAG CCCAGTCTGA CCTGAACAAG ACACGTCTGC GCAGTGGCAG TGCCCTCCTG
    CAGTCTCAGT CCAGTACTGA GGATCCAAAG GATGAGCCTG CAGAGCTGAA GCAAGATCCT
    GAGGACGTAC CTGCCCAGTC CTCCGCATCA AAGGCATCTC AGGAGGAGGT CAATGAAATT
    AAGTCCAAAC GGGACGAGGA AGAACGAGAA CGAGAAAGGA GGGAGAAAGA GAGGGAGCGA
    GAAAGAGAGC GGGAGAAGGA AAAGGAGAGG GAACGAGAGA AGCAGAAACT GAAAGAGTCA
    GAAAAAGAAA GAGACTCTGC TAAGGATAAA GAGAAAGGGA AACATGACGA TGGAAGGAAA
    AAGGAAGCAG AAGTTATCAA ACAATTGAAG ATTGAACTCA AGAAGGCACA AGAGAGCCAA
    AAGGAGATGA AACTATTGCT AGATATGTAC CGCTCTGCCC CAAAGGAACA GAGAGACAAA
    GTTCAGCTGA TGGCAGCTGA GAAGAAGTCA AAGGCAGAGT TGGAAGATTT AAGGCAAAGA
    CTCAAGGATC TAGAGGATAA GGAGAAAAAG GAGAACAAGA AAATGGCTGA TGAGGATGCC
    TTGAGGAAGA TCCGGGCAGT AGAAGAGCAG ATAGAGTACC TGCAGAAGAA GCTGGCCATG
    GCCAAGCAGG AGGAAGAGGC ACTCCTCTCA GAGATGGATG TTACAGGCCA GGCCTTTGAA
    GACATGCAGG AGCAAAATAT CCGTTTAATG CAGCAGTTGC GGGAGAAGGA TGATGCAAAT
    TTCAAGCTCA TGTCTGAGCG TATCAAGTCC AATCAGATCC ACAAGTTGCT CAAAGAAGAG
    AAGGAGGAGC TGGCTGACCA AGTTTTGACT CTAAAGACTC AGGTTGATGC CCAGTTACAG
    GTAGTAAGGA AATTGGAAGA GAAGGAGCAT CTGTTACAAA GCAACATTGG CACAGGGGAG
    AAGGAACTGG GTCTTAGGAC ACAAGCCTTA GAGATGAATA AACGCAAGGC GATGGAAGCA
    GCCCAGCTGG CAGATGACCT CAAAGCGCAA CTGGAATTGG CTCAGAAGAA GCTACATGAT
    TTTCAGGATG AGATCGTGGA GAATAGTGTC ACCAAAGAAA AGGATATGTT TAATTTCAAA
    CGAGCTCAGG AGGACATCTC TAGACTCCGA AGGAAGCTGG AGACCACAAA GAAACCAGAC
    AATGTGCCCA AATGTGATGA GATCCTGATG GAGGAGATTA AGGATTACAA GGCACGCCTG
    ACCTGTCCAT GTTGCAATAT GCGTAAAAAG GATGCTGTAC TTACCAAGTG TTTTCATGTT
    TTCTGCTTTG AGTGTGTAAA GACACGCTAT GACACCCGTC AGCGCAAATG TCCCAAGTGT
    AATGCTGCGT TTGGTGCCAA TGATTTCCAC CGCATCTACA TTGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe14708
    Clone ID Related Accession (Same CDS sequence) XM_005626880.2 , XM_022425660.1 , XM_005626880.3 , XM_532018.4 , XM_532018.5
    Accession Version XM_532018.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2928bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase BRE1A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_532018.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTCAGGAA TTGGAAATAA AAGAGCAGCT GGAGAGCCTG GCACATCCAT GCCTCCGGAG 
    AAGAAGACAG CCGTTGAAGA TTCAGGGACC ACAGTGGAGA CAATTAAGCT TGGAGGTGTC
    TCCTCCACGG AGGAGCTAGA CATTCGAACA CTGCAGACCA AAAATCGCAA GCTGGCAGAA
    ATGCTGGATC AACGGCAGGC CATCGAAGAT GAACTCCGTG AGCACATTGA AAAACTGGAG
    CGAAGACAGG CCACTGATGA TGCCTCACTG CTGATCGTCA ACCGGTACTG GAGTCAGTTT
    GATGAAAACA TCCGTATCAT CCTTAAACGG TACGATCTGG AGCAGGGTTT GGGAGATCTC
    CTCACAGAAA GGAAAGCCCT TGTCGTGCCC GAACCAGAGC CGGACTCTGA TAGCAATCAG
    GAACGGAAAG ATGACCGAGA GAGAGGGGAA GGGCAAGAGC CAGCTTTCTC TTTCCTTGCT
    ACTTTGGCCA GCAGTTCCAG TGAAGAAATG GAATCTCAGC TGCAGGAGCG AGTGGAGTCC
    TCCCGCCGAG CTGTGTCCCA GATCGTGACT GTCTATGATA AATTACAAGA GAAAGTGGAG
    CTGTTATCAT GCAAACTAAA TAGTGGAGAT AATCTGATAG TGGAAGAGGC GGTGCAGGAG
    CTGAATTCCT TCCTTGCACA GGAGAATATG AGGCTACAGG AATTGACAGA CCTCCTTCAG
    GAGAAGCATC GCACCATGTC TCAGGAGTTC TCCAAGTTAC AGAGTAAAGT GGAGACAGCC
    GAGTCGCGAG TGTCTGTCCT AGAGTCCATG ATCGATGACC TGCAGTGGGA TATTGACAAA
    ATTCGAAAGA GGGAACAACG ACTCAACCGA CACTTAGCAG AAGTTCTTGA ACGGGTGAAT
    TCGAAAGGTT ATAAGGTCTA TGGAGCCGGG AGCAGTCTCT ATGGTGGCAC AATCACTATC
    AATGCCCGGA AGTTTGAGGA AATGAATGCA GAGCTGGAGG AGAACAAAGA GTTGGCTCAG
    AACCGTCACT GTGAGCTGGA GAAACTTCGG CAAGACTTGG AGGAGGTCAC TACGCAAAAT
    GAAAAGCTGA AGGTGGAATT GCGGAGCGCG GTGGAGGAGG CAGTTAAGGA GACTCCGGAA
    TACCGCTGTA TGCAGTCACA GTTCTCCGTC CTGTACAATG AGAGCCTGCA GTTGAAAGCG
    CACTTGGATG AGGCTCGGAC TCTGCTTCAT GGCACCAGGG GGACCCACCA GCGCCAAGTT
    GAGCTTATTG AGCGAGATGA AGTTAGTCTT CATAAGAAGC TGAGGACTGA AGTGATCCAG
    CTGGAAGATA CTCTGGCCCA GGTCCGCAAG GAGTATGAGA TGCTGAGGAT AGAATTTGAG
    CAGACCCTTG CTGCCAATGA GCAAGCAGGC CCCATAAACC GGGAGATGCG TCACCTCATC
    AGTAGCCTCC AGAATCACAA TCACCAGCTG AAAGGGGAGG TCCTAAGATA TAAGCGGAAA
    CTGAGAGAAG CCCAGTCTGA CCTGAACAAG ACACGTCTGC GCAGTGGCAG TGCCCTCCTG
    CAGTCTCAGT CCAGTACTGA GGATCCAAAG GATGAGCCTG CAGAGCTGAA GCAAGATCCT
    GAGGACGTAC CTGCCCAGTC CTCCGCATCA AAGGCATCTC AGGAGGAGGT CAATGAAATT
    AAGTCCAAAC GGGACGAGGA AGAACGAGAA CGAGAAAGGA GGGAGAAAGA GAGGGAGCGA
    GAAAGAGAGC GGGAGAAGGA AAAGGAGAGG GAACGAGAGA AGCAGAAACT GAAAGAGTCA
    GAAAAAGAAA GAGACTCTGC TAAGGATAAA GAGAAAGGGA AACATGACGA TGGAAGGAAA
    AAGGAAGCAG AAGTTATCAA ACAATTGAAG ATTGAACTCA AGAAGGCACA AGAGAGCCAA
    AAGGAGATGA AACTATTGCT AGATATGTAC CGCTCTGCCC CAAAGGAACA GAGAGACAAA
    GTTCAGCTGA TGGCAGCTGA GAAGAAGTCA AAGGCAGAGT TGGAAGATTT AAGGCAAAGA
    CTCAAGGATC TAGAGGATAA GGAGAAAAAG GAGAACAAGA AAATGGCTGA TGAGGATGCC
    TTGAGGAAGA TCCGGGCAGT AGAAGAGCAG ATAGAGTACC TGCAGAAGAA GCTGGCCATG
    GCCAAGCAGG AGGAAGAGGC ACTCCTCTCA GAGATGGATG TTACAGGCCA GGCCTTTGAA
    GACATGCAGG AGCAAAATAT CCGTTTAATG CAGCAGTTGC GGGAGAAGGA TGATGCAAAT
    TTCAAGCTCA TGTCTGAGCG TATCAAGTCC AATCAGATCC ACAAGTTGCT CAAAGAAGAG
    AAGGAGGAGC TGGCTGACCA AGTTTTGACT CTAAAGACTC AGGTTGATGC CCAGTTACAG
    GTAGTAAGGA AATTGGAAGA GAAGGAGCAT CTGTTACAAA GCAACATTGG CACAGGGGAG
    AAGGAACTGG GTCTTAGGAC ACAAGCCTTA GAGATGAATA AACGCAAGGC GATGGAAGCA
    GCCCAGCTGG CAGATGACCT CAAAGCGCAA CTGGAATTGG CTCAGAAGAA GCTACATGAT
    TTTCAGGATG AGATCGTGGA GAATAGTGTC ACCAAAGAAA AGGATATGTT TAATTTCAAA
    CGAGCTCAGG AGGACATCTC TAGACTCCGA AGGAAGCTGG AGACCACAAA GAAACCAGAC
    AATGTGCCCA AATGTGATGA GATCCTGATG GAGGAGATTA AGGATTACAA GGCACGCCTG
    ACCTGTCCAT GTTGCAATAT GCGTAAAAAG GATGCTGTAC TTACCAAGTG TTTTCATGTT
    TTCTGCTTTG AGTGTGTAAA GACACGCTAT GACACCCGTC AGCGCAAATG TCCCAAGTGT
    AATGCTGCGT TTGGTGCCAA TGATTTCCAC CGCATCTACA TTGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_532018.3
    CDS180..3107
    Translation

    Target ORF information:

    RefSeq Version XM_532018.5
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ring finger protein 20 (RNF20), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_532018.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTCAGGAA TTGGAAATAA AAGAGCAGCT GGAGAGCCTG GCACATCCAT GCCTCCGGAG 
    AAGAAGACAG CCGTTGAAGA TTCAGGGACC ACAGTGGAGA CAATTAAGCT TGGAGGTGTC
    TCCTCCACGG AGGAGCTAGA CATTCGAACA CTGCAGACCA AAAATCGCAA GCTGGCAGAA
    ATGCTGGATC AACGGCAGGC CATCGAAGAT GAACTCCGTG AGCACATTGA AAAACTGGAG
    CGAAGACAGG CCACTGATGA TGCCTCACTG CTGATCGTCA ACCGGTACTG GAGTCAGTTT
    GATGAAAACA TCCGTATCAT CCTTAAACGG TACGATCTGG AGCAGGGTTT GGGAGATCTC
    CTCACAGAAA GGAAAGCCCT TGTCGTGCCC GAACCAGAGC CGGACTCTGA TAGCAATCAG
    GAACGGAAAG ATGACCGAGA GAGAGGGGAA GGGCAAGAGC CAGCTTTCTC TTTCCTTGCT
    ACTTTGGCCA GCAGTTCCAG TGAAGAAATG GAATCTCAGC TGCAGGAGCG AGTGGAGTCC
    TCCCGCCGAG CTGTGTCCCA GATCGTGACT GTCTATGATA AATTACAAGA GAAAGTGGAG
    CTGTTATCAT GCAAACTAAA TAGTGGAGAT AATCTGATAG TGGAAGAGGC GGTGCAGGAG
    CTGAATTCCT TCCTTGCACA GGAGAATATG AGGCTACAGG AATTGACAGA CCTCCTTCAG
    GAGAAGCATC GCACCATGTC TCAGGAGTTC TCCAAGTTAC AGAGTAAAGT GGAGACAGCC
    GAGTCGCGAG TGTCTGTCCT AGAGTCCATG ATCGATGACC TGCAGTGGGA TATTGACAAA
    ATTCGAAAGA GGGAACAACG ACTCAACCGA CACTTAGCAG AAGTTCTTGA ACGGGTGAAT
    TCGAAAGGTT ATAAGGTCTA TGGAGCCGGG AGCAGTCTCT ATGGTGGCAC AATCACTATC
    AATGCCCGGA AGTTTGAGGA AATGAATGCA GAGCTGGAGG AGAACAAAGA GTTGGCTCAG
    AACCGTCACT GTGAGCTGGA GAAACTTCGG CAAGACTTGG AGGAGGTCAC TACGCAAAAT
    GAAAAGCTGA AGGTGGAATT GCGGAGCGCG GTGGAGGAGG CAGTTAAGGA GACTCCGGAA
    TACCGCTGTA TGCAGTCACA GTTCTCCGTC CTGTACAATG AGAGCCTGCA GTTGAAAGCG
    CACTTGGATG AGGCTCGGAC TCTGCTTCAT GGCACCAGGG GGACCCACCA GCGCCAAGTT
    GAGCTTATTG AGCGAGATGA AGTTAGTCTT CATAAGAAGC TGAGGACTGA AGTGATCCAG
    CTGGAAGATA CTCTGGCCCA GGTCCGCAAG GAGTATGAGA TGCTGAGGAT AGAATTTGAG
    CAGACCCTTG CTGCCAATGA GCAAGCAGGC CCCATAAACC GGGAGATGCG TCACCTCATC
    AGTAGCCTCC AGAATCACAA TCACCAGCTG AAAGGGGAGG TCCTAAGATA TAAGCGGAAA
    CTGAGAGAAG CCCAGTCTGA CCTGAACAAG ACACGTCTGC GCAGTGGCAG TGCCCTCCTG
    CAGTCTCAGT CCAGTACTGA GGATCCAAAG GATGAGCCTG CAGAGCTGAA GCAAGATCCT
    GAGGACGTAC CTGCCCAGTC CTCCGCATCA AAGGCATCTC AGGAGGAGGT CAATGAAATT
    AAGTCCAAAC GGGACGAGGA AGAACGAGAA CGAGAAAGGA GGGAGAAAGA GAGGGAGCGA
    GAAAGAGAGC GGGAGAAGGA AAAGGAGAGG GAACGAGAGA AGCAGAAACT GAAAGAGTCA
    GAAAAAGAAA GAGACTCTGC TAAGGATAAA GAGAAAGGGA AACATGACGA TGGAAGGAAA
    AAGGAAGCAG AAGTTATCAA ACAATTGAAG ATTGAACTCA AGAAGGCACA AGAGAGCCAA
    AAGGAGATGA AACTATTGCT AGATATGTAC CGCTCTGCCC CAAAGGAACA GAGAGACAAA
    GTTCAGCTGA TGGCAGCTGA GAAGAAGTCA AAGGCAGAGT TGGAAGATTT AAGGCAAAGA
    CTCAAGGATC TAGAGGATAA GGAGAAAAAG GAGAACAAGA AAATGGCTGA TGAGGATGCC
    TTGAGGAAGA TCCGGGCAGT AGAAGAGCAG ATAGAGTACC TGCAGAAGAA GCTGGCCATG
    GCCAAGCAGG AGGAAGAGGC ACTCCTCTCA GAGATGGATG TTACAGGCCA GGCCTTTGAA
    GACATGCAGG AGCAAAATAT CCGTTTAATG CAGCAGTTGC GGGAGAAGGA TGATGCAAAT
    TTCAAGCTCA TGTCTGAGCG TATCAAGTCC AATCAGATCC ACAAGTTGCT CAAAGAAGAG
    AAGGAGGAGC TGGCTGACCA AGTTTTGACT CTAAAGACTC AGGTTGATGC CCAGTTACAG
    GTAGTAAGGA AATTGGAAGA GAAGGAGCAT CTGTTACAAA GCAACATTGG CACAGGGGAG
    AAGGAACTGG GTCTTAGGAC ACAAGCCTTA GAGATGAATA AACGCAAGGC GATGGAAGCA
    GCCCAGCTGG CAGATGACCT CAAAGCGCAA CTGGAATTGG CTCAGAAGAA GCTACATGAT
    TTTCAGGATG AGATCGTGGA GAATAGTGTC ACCAAAGAAA AGGATATGTT TAATTTCAAA
    CGAGCTCAGG AGGACATCTC TAGACTCCGA AGGAAGCTGG AGACCACAAA GAAACCAGAC
    AATGTGCCCA AATGTGATGA GATCCTGATG GAGGAGATTA AGGATTACAA GGCACGCCTG
    ACCTGTCCAT GTTGCAATAT GCGTAAAAAG GATGCTGTAC TTACCAAGTG TTTTCATGTT
    TTCTGCTTTG AGTGTGTAAA GACACGCTAT GACACCCGTC AGCGCAAATG TCCCAAGTGT
    AATGCTGCGT TTGGTGCCAA TGATTTCCAC CGCATCTACA TTGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe292198
    Clone ID Related Accession (Same CDS sequence) XM_022425661.1
    Accession Version XM_022425661.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2841bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase BRE1A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGGGAGG AAAGTGTTGG AGGTTTGAAG AGACAGGAGA AGGAGGAGCT AGACATTCGA 
    ACACTGCAGA CCAAAAATCG CAAGCTGGCA GAAATGCTGG ATCAACGGCA GGCCATCGAA
    GATGAACTCC GTGAGCACAT TGAAAAACTG GAGCGAAGAC AGGCCACTGA TGATGCCTCA
    CTGCTGATCG TCAACCGGTA CTGGAGTCAG TTTGATGAAA ACATCCGTAT CATCCTTAAA
    CGGTACGATC TGGAGCAGGG TTTGGGAGAT CTCCTCACAG AAAGGAAAGC CCTTGTCGTG
    CCCGAACCAG AGCCGGACTC TGATAGCAAT CAGGAACGGA AAGATGACCG AGAGAGAGGG
    GAAGGGCAAG AGCCAGCTTT CTCTTTCCTT GCTACTTTGG CCAGCAGTTC CAGTGAAGAA
    ATGGAATCTC AGCTGCAGGA GCGAGTGGAG TCCTCCCGCC GAGCTGTGTC CCAGATCGTG
    ACTGTCTATG ATAAATTACA AGAGAAAGTG GAGCTGTTAT CATGCAAACT AAATAGTGGA
    GATAATCTGA TAGTGGAAGA GGCGGTGCAG GAGCTGAATT CCTTCCTTGC ACAGGAGAAT
    ATGAGGCTAC AGGAATTGAC AGACCTCCTT CAGGAGAAGC ATCGCACCAT GTCTCAGGAG
    TTCTCCAAGT TACAGAGTAA AGTGGAGACA GCCGAGTCGC GAGTGTCTGT CCTAGAGTCC
    ATGATCGATG ACCTGCAGTG GGATATTGAC AAAATTCGAA AGAGGGAACA ACGACTCAAC
    CGACACTTAG CAGAAGTTCT TGAACGGGTG AATTCGAAAG GTTATAAGGT CTATGGAGCC
    GGGAGCAGTC TCTATGGTGG CACAATCACT ATCAATGCCC GGAAGTTTGA GGAAATGAAT
    GCAGAGCTGG AGGAGAACAA AGAGTTGGCT CAGAACCGTC ACTGTGAGCT GGAGAAACTT
    CGGCAAGACT TGGAGGAGGT CACTACGCAA AATGAAAAGC TGAAGGTGGA ATTGCGGAGC
    GCGGTGGAGG AGGCAGTTAA GGAGACTCCG GAATACCGCT GTATGCAGTC ACAGTTCTCC
    GTCCTGTACA ATGAGAGCCT GCAGTTGAAA GCGCACTTGG ATGAGGCTCG GACTCTGCTT
    CATGGCACCA GGGGGACCCA CCAGCGCCAA GTTGAGCTTA TTGAGCGAGA TGAAGTTAGT
    CTTCATAAGA AGCTGAGGAC TGAAGTGATC CAGCTGGAAG ATACTCTGGC CCAGGTCCGC
    AAGGAGTATG AGATGCTGAG GATAGAATTT GAGCAGACCC TTGCTGCCAA TGAGCAAGCA
    GGCCCCATAA ACCGGGAGAT GCGTCACCTC ATCAGTAGCC TCCAGAATCA CAATCACCAG
    CTGAAAGGGG AGGTCCTAAG ATATAAGCGG AAACTGAGAG AAGCCCAGTC TGACCTGAAC
    AAGACACGTC TGCGCAGTGG CAGTGCCCTC CTGCAGTCTC AGTCCAGTAC TGAGGATCCA
    AAGGATGAGC CTGCAGAGCT GAAGCAAGAT CCTGAGGACG TACCTGCCCA GTCCTCCGCA
    TCAAAGGCAT CTCAGGAGGA GGTCAATGAA ATTAAGTCCA AACGGGACGA GGAAGAACGA
    GAACGAGAAA GGAGGGAGAA AGAGAGGGAG CGAGAAAGAG AGCGGGAGAA GGAAAAGGAG
    AGGGAACGAG AGAAGCAGAA ACTGAAAGAG TCAGAAAAAG AAAGAGACTC TGCTAAGGAT
    AAAGAGAAAG GGAAACATGA CGATGGAAGG AAAAAGGAAG CAGAAGTTAT CAAACAATTG
    AAGATTGAAC TCAAGAAGGC ACAAGAGAGC CAAAAGGAGA TGAAACTATT GCTAGATATG
    TACCGCTCTG CCCCAAAGGA ACAGAGAGAC AAAGTTCAGC TGATGGCAGC TGAGAAGAAG
    TCAAAGGCAG AGTTGGAAGA TTTAAGGCAA AGACTCAAGG ATCTAGAGGA TAAGGAGAAA
    AAGGAGAACA AGAAAATGGC TGATGAGGAT GCCTTGAGGA AGATCCGGGC AGTAGAAGAG
    CAGATAGAGT ACCTGCAGAA GAAGCTGGCC ATGGCCAAGC AGGAGGAAGA GGCACTCCTC
    TCAGAGATGG ATGTTACAGG CCAGGCCTTT GAAGACATGC AGGAGCAAAA TATCCGTTTA
    ATGCAGCAGT TGCGGGAGAA GGATGATGCA AATTTCAAGC TCATGTCTGA GCGTATCAAG
    TCCAATCAGA TCCACAAGTT GCTCAAAGAA GAGAAGGAGG AGCTGGCTGA CCAAGTTTTG
    ACTCTAAAGA CTCAGGTTGA TGCCCAGTTA CAGGTAGTAA GGAAATTGGA AGAGAAGGAG
    CATCTGTTAC AAAGCAACAT TGGCACAGGG GAGAAGGAAC TGGGTCTTAG GACACAAGCC
    TTAGAGATGA ATAAACGCAA GGCGATGGAA GCAGCCCAGC TGGCAGATGA CCTCAAAGCG
    CAACTGGAAT TGGCTCAGAA GAAGCTACAT GATTTTCAGG ATGAGATCGT GGAGAATAGT
    GTCACCAAAG AAAAGGATAT GTTTAATTTC AAACGAGCTC AGGAGGACAT CTCTAGACTC
    CGAAGGAAGC TGGAGACCAC AAAGAAACCA GACAATGTGC CCAAATGTGA TGAGATCCTG
    ATGGAGGAGA TTAAGGATTA CAAGGCACGC CTGACCTGTC CATGTTGCAA TATGCGTAAA
    AAGGATGCTG TACTTACCAA GTGTTTTCAT GTTTTCTGCT TTGAGTGTGT AAAGACACGC
    TATGACACCC GTCAGCGCAA ATGTCCCAAG TGTAATGCTG CGTTTGGTGC CAATGATTTC
    CACCGCATCT ACATTGGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022281369.1
    CDS169..3009
    Translation

    Target ORF information:

    RefSeq Version XM_022425661.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ring finger protein 20 (RNF20), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022425661.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGGGAGG AAAGTGTTGG AGGTTTGAAG AGACAGGAGA AGGAGGAGCT AGACATTCGA 
    ACACTGCAGA CCAAAAATCG CAAGCTGGCA GAAATGCTGG ATCAACGGCA GGCCATCGAA
    GATGAACTCC GTGAGCACAT TGAAAAACTG GAGCGAAGAC AGGCCACTGA TGATGCCTCA
    CTGCTGATCG TCAACCGGTA CTGGAGTCAG TTTGATGAAA ACATCCGTAT CATCCTTAAA
    CGGTACGATC TGGAGCAGGG TTTGGGAGAT CTCCTCACAG AAAGGAAAGC CCTTGTCGTG
    CCCGAACCAG AGCCGGACTC TGATAGCAAT CAGGAACGGA AAGATGACCG AGAGAGAGGG
    GAAGGGCAAG AGCCAGCTTT CTCTTTCCTT GCTACTTTGG CCAGCAGTTC CAGTGAAGAA
    ATGGAATCTC AGCTGCAGGA GCGAGTGGAG TCCTCCCGCC GAGCTGTGTC CCAGATCGTG
    ACTGTCTATG ATAAATTACA AGAGAAAGTG GAGCTGTTAT CATGCAAACT AAATAGTGGA
    GATAATCTGA TAGTGGAAGA GGCGGTGCAG GAGCTGAATT CCTTCCTTGC ACAGGAGAAT
    ATGAGGCTAC AGGAATTGAC AGACCTCCTT CAGGAGAAGC ATCGCACCAT GTCTCAGGAG
    TTCTCCAAGT TACAGAGTAA AGTGGAGACA GCCGAGTCGC GAGTGTCTGT CCTAGAGTCC
    ATGATCGATG ACCTGCAGTG GGATATTGAC AAAATTCGAA AGAGGGAACA ACGACTCAAC
    CGACACTTAG CAGAAGTTCT TGAACGGGTG AATTCGAAAG GTTATAAGGT CTATGGAGCC
    GGGAGCAGTC TCTATGGTGG CACAATCACT ATCAATGCCC GGAAGTTTGA GGAAATGAAT
    GCAGAGCTGG AGGAGAACAA AGAGTTGGCT CAGAACCGTC ACTGTGAGCT GGAGAAACTT
    CGGCAAGACT TGGAGGAGGT CACTACGCAA AATGAAAAGC TGAAGGTGGA ATTGCGGAGC
    GCGGTGGAGG AGGCAGTTAA GGAGACTCCG GAATACCGCT GTATGCAGTC ACAGTTCTCC
    GTCCTGTACA ATGAGAGCCT GCAGTTGAAA GCGCACTTGG ATGAGGCTCG GACTCTGCTT
    CATGGCACCA GGGGGACCCA CCAGCGCCAA GTTGAGCTTA TTGAGCGAGA TGAAGTTAGT
    CTTCATAAGA AGCTGAGGAC TGAAGTGATC CAGCTGGAAG ATACTCTGGC CCAGGTCCGC
    AAGGAGTATG AGATGCTGAG GATAGAATTT GAGCAGACCC TTGCTGCCAA TGAGCAAGCA
    GGCCCCATAA ACCGGGAGAT GCGTCACCTC ATCAGTAGCC TCCAGAATCA CAATCACCAG
    CTGAAAGGGG AGGTCCTAAG ATATAAGCGG AAACTGAGAG AAGCCCAGTC TGACCTGAAC
    AAGACACGTC TGCGCAGTGG CAGTGCCCTC CTGCAGTCTC AGTCCAGTAC TGAGGATCCA
    AAGGATGAGC CTGCAGAGCT GAAGCAAGAT CCTGAGGACG TACCTGCCCA GTCCTCCGCA
    TCAAAGGCAT CTCAGGAGGA GGTCAATGAA ATTAAGTCCA AACGGGACGA GGAAGAACGA
    GAACGAGAAA GGAGGGAGAA AGAGAGGGAG CGAGAAAGAG AGCGGGAGAA GGAAAAGGAG
    AGGGAACGAG AGAAGCAGAA ACTGAAAGAG TCAGAAAAAG AAAGAGACTC TGCTAAGGAT
    AAAGAGAAAG GGAAACATGA CGATGGAAGG AAAAAGGAAG CAGAAGTTAT CAAACAATTG
    AAGATTGAAC TCAAGAAGGC ACAAGAGAGC CAAAAGGAGA TGAAACTATT GCTAGATATG
    TACCGCTCTG CCCCAAAGGA ACAGAGAGAC AAAGTTCAGC TGATGGCAGC TGAGAAGAAG
    TCAAAGGCAG AGTTGGAAGA TTTAAGGCAA AGACTCAAGG ATCTAGAGGA TAAGGAGAAA
    AAGGAGAACA AGAAAATGGC TGATGAGGAT GCCTTGAGGA AGATCCGGGC AGTAGAAGAG
    CAGATAGAGT ACCTGCAGAA GAAGCTGGCC ATGGCCAAGC AGGAGGAAGA GGCACTCCTC
    TCAGAGATGG ATGTTACAGG CCAGGCCTTT GAAGACATGC AGGAGCAAAA TATCCGTTTA
    ATGCAGCAGT TGCGGGAGAA GGATGATGCA AATTTCAAGC TCATGTCTGA GCGTATCAAG
    TCCAATCAGA TCCACAAGTT GCTCAAAGAA GAGAAGGAGG AGCTGGCTGA CCAAGTTTTG
    ACTCTAAAGA CTCAGGTTGA TGCCCAGTTA CAGGTAGTAA GGAAATTGGA AGAGAAGGAG
    CATCTGTTAC AAAGCAACAT TGGCACAGGG GAGAAGGAAC TGGGTCTTAG GACACAAGCC
    TTAGAGATGA ATAAACGCAA GGCGATGGAA GCAGCCCAGC TGGCAGATGA CCTCAAAGCG
    CAACTGGAAT TGGCTCAGAA GAAGCTACAT GATTTTCAGG ATGAGATCGT GGAGAATAGT
    GTCACCAAAG AAAAGGATAT GTTTAATTTC AAACGAGCTC AGGAGGACAT CTCTAGACTC
    CGAAGGAAGC TGGAGACCAC AAAGAAACCA GACAATGTGC CCAAATGTGA TGAGATCCTG
    ATGGAGGAGA TTAAGGATTA CAAGGCACGC CTGACCTGTC CATGTTGCAA TATGCGTAAA
    AAGGATGCTG TACTTACCAA GTGTTTTCAT GTTTTCTGCT TTGAGTGTGT AAAGACACGC
    TATGACACCC GTCAGCGCAA ATGTCCCAAG TGTAATGCTG CGTTTGGTGC CAATGATTTC
    CACCGCATCT ACATTGGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.