MEGF10 cDNA ORF clone, Canis lupus familiaris(dog)

The following MEGF10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MEGF10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe14211 XM_014117690.2
Latest version!
Canis lupus familiaris multiple EGF like domains 10 (MEGF10), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OCe14211 XM_005626476.3
Latest version!
Canis lupus familiaris multiple EGF like domains 10 (MEGF10), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OCe14211 XM_014117692.1
Latest version!
Canis lupus familiaris multiple EGF-like-domains 10 (MEGF10), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OCe14211 XM_531893.6
Latest version!
Canis lupus familiaris multiple EGF like domains 10 (MEGF10), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OCe14211 XM_014117691.2
Latest version!
Canis lupus familiaris multiple EGF like domains 10 (MEGF10), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OCe14212 XM_022425234.1
Latest version!
Canis lupus familiaris multiple EGF like domains 10 (MEGF10), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OCe14213 XM_005626478.3
Latest version!
Canis lupus familiaris multiple EGF like domains 10 (MEGF10), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OCe291935 XM_022425235.1
Latest version!
Canis lupus familiaris multiple EGF like domains 10 (MEGF10), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
View Old Accession Versions >>
OCe14213 XM_005626478.2 Canis lupus familiaris multiple EGF-like-domains 10 (MEGF10), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OCe14211 XM_014117690.1 Canis lupus familiaris multiple EGF-like-domains 10 (MEGF10), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OCe14211 XM_005626476.2 Canis lupus familiaris multiple EGF-like-domains 10 (MEGF10), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OCe14211 XM_531893.5 Canis lupus familiaris multiple EGF-like-domains 10 (MEGF10), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OCe14211 XM_014117691.1 Canis lupus familiaris multiple EGF-like-domains 10 (MEGF10), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe14211
    Clone ID Related Accession (Same CDS sequence) XM_014117692.1 , XM_014117690.2 , XM_005626476.2 , XM_531893.6 , XM_014117690.1 , XM_014117691.1 , XM_005626476.3 , XM_531893.5 , XM_014117691.2
    Accession Version XM_014117692.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3414bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product multiple epidermal growth factor-like domains protein 10 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGTTGCTT CTTTGAACTC ATGCCTGAGC TTTATCTGTT TCTCATTATG CCTCTGGAGC 
    AGGACAGCAT CAGCACTGAA TCTTGAAGAC CCTAATGTGT GTAGCCACTG GGAAAGCTAC
    TCAGTGACTG TGCAAGAGTC ATACCCACAT CCTTTCGATC AAATCTACTA CACCAGCTGC
    ACTGATATTC TGAACTGGTT TAAATGTACA CGGCACAGAA TCAGTTATCG GACTGCCTAT
    CGACATGGGG AGAAAACTAT GTATAGGCGC AAATCCCAGT GTTGTCCTGG ATTTTATGAA
    AGCAGGGAAA TGTGTGTCCC CCACTGTGCT GATAAATGTG TCCATGGTCG CTGCATTGCT
    CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGTGGAACCA ACTGCTCCAG TGCCTGTGAT
    GGTGATCACT GGGGGCCCCA TTGCAGCAGC CGGTGCCAGT GCAAGAACGG GGCCCTGTGC
    AACCCCATCA CGGGGGCTTG CCACTGCGCT GCAGGTTTCC GGGGCTGGCG TTGTGAGGAC
    CGCTGTGAGC AGGGCACCTA CGGTAATGAC TGTCACCAGA GATGCCAGTG TCAGAATGGA
    GCCACCTGCG ACCACGTCAC AGGGGAATGC CGCTGCCCAC CAGGGTACAC TGGAGCCTTC
    TGTGAGGACC TTTGTCCCCC TGGCAAGCAC GGTCCGCAGT GCGAGCAGAG ATGTCCCTGC
    CAAAATGGAG GAATATGTCA TCACGTCACC GGAGAATGCT CTTGCCCTTC GGGCTGGCTG
    GGCACAGTCT GTGGTCAGCC TTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCACAAGAA
    TGTCAGTGCC ATAATGGAGG GACGTGTGAT GCTGCCACAG GCCAATGTCA TTGCAGTCCA
    GGATACACAG GGGAACGCTG CCAGGACGAG TGTCCCGTTG GCACATACGG AGTTCGCTGT
    GCTGAGACCT GCCAGTGTGT CAATGGAGGG AAGTGTTATC ACGTGAGTGG CTCATGCCTC
    TGTGAAGCAG GCTTTGCTGG TGAGCACTGT GAGGCACGCC TGTGTCCCGA AGGGCTCTAC
    GGCATCAAAT GTGACAAGCG GTGTCCCTGC CACTTGGACA ACACTCATAG CTGTCACCCC
    ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC TGGTCAGGAC TCTACTGTAA CGAAACATGT
    TCTCCTGGAT TCTTTGGGGA AGCTTGCCAA CAGATCTGCA GCTGCCACAA CGGGGCTGAT
    TGTGACAGTG TGACTGGAAA ATGCATCTGT GCCCCAGGAT TCAAAGGAAT TGACTGCTCT
    ATCCCATGCC CATTGGAAAC CTATGGGATA AACTGCTCCT CTCTCTGTGG CTGTAAAAAC
    GATGCTGTCT GTTCACCTGT AGATGGGTCT TGTACTTGCA AGGCAGGCTG GCACGGGGTG
    GACTGCTCCA TCAGCTGTCC CAGTGGCACG TGGGGCTTCA GCTGTAACCT GACCTGCCAG
    TGCCTCCACG GGGGAGCCTG CAACACCCAG GACGGGACCT GCACGTGTGC GCCTGGATGG
    CGCGGGGACA AATGTGAACT CCCCTGCCAG GATGGCACAT ATGGGCTGAA CTGTGCGGAG
    CGCTGTGACT GTAGCCATGC GGATGGCTGC CACTCCACCA CGGGCCACTG CCGCTGCCTC
    CCTGGATGGT CAGGTGTCCA CTGCGACAGC GTGTGTGCTG AGGGGCGCTG GGGGCCCAAC
    TGCTCTCTGC CCTGCTACTG TAAAAATGGG GCTTCATGCT CCCCAGACGA TGGCATCTGT
    GAGTGTGCGC CAGGGTTCCG AGGCACCACC TGTCAGAGAA TCTGCTCCCC TGGTTTTTAT
    GGGCATCGCT GCAGCCAGAC ATGCCCGCAG TGCGTGCATA GCAGTGGGCC CTGCCACCAC
    ATCACAGGCC TGTGTGACTG CTTGCCTGGC TTCACAGGTG CCCTCTGCAA TGAAGTGTGT
    CCCAGTGGCA GATTTGGGAA AAACTGTGCT GGAGTTTGTA CCTGTACAAA CAATGGAACC
    TGTAACCCCA TTGACCGATC TTGTCAGTGT TATCCAGGGT GGATTGGCAG TGACTGCTCG
    CAACCTTGTC CTCCTGCGCA TTGGGGTCCA AACTGCATCC ACACGTGCAA CTGCCACAAT
    GGGGCGTTCT GCAGTGCATA CGACGGGGAA TGTAAATGCA CTCCTGGCTG GACCGGACTC
    TACTGCACTC AGAGATGTCC CCTGGGTTTT TACGGAAAGG ACTGTGCGTT GATATGTCAG
    TGTCAGAATG GAGCTGACTG TGACCACATC TCTGGGCAGT GTACATGCCG CACTGGGTTC
    ATGGGGAAGC ACTGTGAACA GAAATGCCCT GCGGGAACAT ATGGCTATGG CTGTCGCCAG
    ATATGTGACT GTCTGAACAA CTCCACCTGT GACCATATCA CTGGGACTTG TTACTGTAGC
    CCAGGCTGGA AGGGGGCACG GTGTGATCAA GCTGGAGTCA TTATCGTTGG AAATCTGAAC
    AGCTTAAGCA GGACCAGTAC AGCTCTCCCT GCCAATTCCT ACCAGATCGG AGCCATCGTG
    GGCATCATCA TTCTTGTACT GGTTGTTCTC TTCCTCCTGG CGTTGTTCAT TATTTACAGA
    CAGAAGCAAA AGGGAAAGGA ATCAAGCATG CCTGCAGTTA CCTACACCCC TGCCCTGAGG
    GTCATGAATG CAGACTATAC CATTTCAGAA ACCCTTCCCC ACAGCAACAG TGGAAGTGCT
    AACAGCCATT ACTTCACTAA CCCCAGTTAC CACACACTTA CCCAGTGCGC CACATCCCCA
    CATGTCAACA ACAGGGACAG GATGACCATC GCAAAGTCAA AGAACAATCA GCTGTTTGTG
    AATCTGAAAA ATGTGAATCC TGGGAAGAGA GGCCCAGTGG CAGACTGCAC GGGAACATTG
    CCAGCAGACT GGAAACATGG CGGCTACCTT AATGAGCTTG GAGCTTTTGG ACTTGATAGA
    AGTTATATGG GAAAATCCTT GAAAGATTTG GGAAAGAATT CTGAATATAA TTCAAGTAAC
    TGCTCCTTAA GCAGTTCTGA AAACCCATAT GCCACTATTA AAGACCCGCC TGTACTTATC
    CCCAAAAACT CAGAGTGTGG CTATGTGGAA ATGAAGTCAC CAGCACGGAG AGATTCCCCC
    TATGCAGAGA TCAATAACTC AACTTCAGCC AACAAGAATG TCTATGAAGT TGAACCTACA
    GTGAGTGTTG TCCAAGGAAT ATTCAGCAAT AATGGGCATC TCACCCAGGA TCCATACGAC
    CTCCCAAAGA ACAGTCACAT CCCTTGCCAT TATGACCTGC TGCCAGTCCG AGACAGTTCA
    TCCTCCCCCA AGCGAGAGGA TGGTGGCAGT GGCAGCAGCA GCAGCAGTGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973167.1
    CDS38..3451
    Translation

    Target ORF information:

    RefSeq Version XM_014117692.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris multiple EGF-like-domains 10 (MEGF10), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014117692.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGTTGCTT CTTTGAACTC ATGCCTGAGC TTTATCTGTT TCTCATTATG CCTCTGGAGC 
    AGGACAGCAT CAGCACTGAA TCTTGAAGAC CCTAATGTGT GTAGCCACTG GGAAAGCTAC
    TCAGTGACTG TGCAAGAGTC ATACCCACAT CCTTTCGATC AAATCTACTA CACCAGCTGC
    ACTGATATTC TGAACTGGTT TAAATGTACA CGGCACAGAA TCAGTTATCG GACTGCCTAT
    CGACATGGGG AGAAAACTAT GTATAGGCGC AAATCCCAGT GTTGTCCTGG ATTTTATGAA
    AGCAGGGAAA TGTGTGTCCC CCACTGTGCT GATAAATGTG TCCATGGTCG CTGCATTGCT
    CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGTGGAACCA ACTGCTCCAG TGCCTGTGAT
    GGTGATCACT GGGGGCCCCA TTGCAGCAGC CGGTGCCAGT GCAAGAACGG GGCCCTGTGC
    AACCCCATCA CGGGGGCTTG CCACTGCGCT GCAGGTTTCC GGGGCTGGCG TTGTGAGGAC
    CGCTGTGAGC AGGGCACCTA CGGTAATGAC TGTCACCAGA GATGCCAGTG TCAGAATGGA
    GCCACCTGCG ACCACGTCAC AGGGGAATGC CGCTGCCCAC CAGGGTACAC TGGAGCCTTC
    TGTGAGGACC TTTGTCCCCC TGGCAAGCAC GGTCCGCAGT GCGAGCAGAG ATGTCCCTGC
    CAAAATGGAG GAATATGTCA TCACGTCACC GGAGAATGCT CTTGCCCTTC GGGCTGGCTG
    GGCACAGTCT GTGGTCAGCC TTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCACAAGAA
    TGTCAGTGCC ATAATGGAGG GACGTGTGAT GCTGCCACAG GCCAATGTCA TTGCAGTCCA
    GGATACACAG GGGAACGCTG CCAGGACGAG TGTCCCGTTG GCACATACGG AGTTCGCTGT
    GCTGAGACCT GCCAGTGTGT CAATGGAGGG AAGTGTTATC ACGTGAGTGG CTCATGCCTC
    TGTGAAGCAG GCTTTGCTGG TGAGCACTGT GAGGCACGCC TGTGTCCCGA AGGGCTCTAC
    GGCATCAAAT GTGACAAGCG GTGTCCCTGC CACTTGGACA ACACTCATAG CTGTCACCCC
    ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC TGGTCAGGAC TCTACTGTAA CGAAACATGT
    TCTCCTGGAT TCTTTGGGGA AGCTTGCCAA CAGATCTGCA GCTGCCACAA CGGGGCTGAT
    TGTGACAGTG TGACTGGAAA ATGCATCTGT GCCCCAGGAT TCAAAGGAAT TGACTGCTCT
    ATCCCATGCC CATTGGAAAC CTATGGGATA AACTGCTCCT CTCTCTGTGG CTGTAAAAAC
    GATGCTGTCT GTTCACCTGT AGATGGGTCT TGTACTTGCA AGGCAGGCTG GCACGGGGTG
    GACTGCTCCA TCAGCTGTCC CAGTGGCACG TGGGGCTTCA GCTGTAACCT GACCTGCCAG
    TGCCTCCACG GGGGAGCCTG CAACACCCAG GACGGGACCT GCACGTGTGC GCCTGGATGG
    CGCGGGGACA AATGTGAACT CCCCTGCCAG GATGGCACAT ATGGGCTGAA CTGTGCGGAG
    CGCTGTGACT GTAGCCATGC GGATGGCTGC CACTCCACCA CGGGCCACTG CCGCTGCCTC
    CCTGGATGGT CAGGTGTCCA CTGCGACAGC GTGTGTGCTG AGGGGCGCTG GGGGCCCAAC
    TGCTCTCTGC CCTGCTACTG TAAAAATGGG GCTTCATGCT CCCCAGACGA TGGCATCTGT
    GAGTGTGCGC CAGGGTTCCG AGGCACCACC TGTCAGAGAA TCTGCTCCCC TGGTTTTTAT
    GGGCATCGCT GCAGCCAGAC ATGCCCGCAG TGCGTGCATA GCAGTGGGCC CTGCCACCAC
    ATCACAGGCC TGTGTGACTG CTTGCCTGGC TTCACAGGTG CCCTCTGCAA TGAAGTGTGT
    CCCAGTGGCA GATTTGGGAA AAACTGTGCT GGAGTTTGTA CCTGTACAAA CAATGGAACC
    TGTAACCCCA TTGACCGATC TTGTCAGTGT TATCCAGGGT GGATTGGCAG TGACTGCTCG
    CAACCTTGTC CTCCTGCGCA TTGGGGTCCA AACTGCATCC ACACGTGCAA CTGCCACAAT
    GGGGCGTTCT GCAGTGCATA CGACGGGGAA TGTAAATGCA CTCCTGGCTG GACCGGACTC
    TACTGCACTC AGAGATGTCC CCTGGGTTTT TACGGAAAGG ACTGTGCGTT GATATGTCAG
    TGTCAGAATG GAGCTGACTG TGACCACATC TCTGGGCAGT GTACATGCCG CACTGGGTTC
    ATGGGGAAGC ACTGTGAACA GAAATGCCCT GCGGGAACAT ATGGCTATGG CTGTCGCCAG
    ATATGTGACT GTCTGAACAA CTCCACCTGT GACCATATCA CTGGGACTTG TTACTGTAGC
    CCAGGCTGGA AGGGGGCACG GTGTGATCAA GCTGGAGTCA TTATCGTTGG AAATCTGAAC
    AGCTTAAGCA GGACCAGTAC AGCTCTCCCT GCCAATTCCT ACCAGATCGG AGCCATCGTG
    GGCATCATCA TTCTTGTACT GGTTGTTCTC TTCCTCCTGG CGTTGTTCAT TATTTACAGA
    CAGAAGCAAA AGGGAAAGGA ATCAAGCATG CCTGCAGTTA CCTACACCCC TGCCCTGAGG
    GTCATGAATG CAGACTATAC CATTTCAGAA ACCCTTCCCC ACAGCAACAG TGGAAGTGCT
    AACAGCCATT ACTTCACTAA CCCCAGTTAC CACACACTTA CCCAGTGCGC CACATCCCCA
    CATGTCAACA ACAGGGACAG GATGACCATC GCAAAGTCAA AGAACAATCA GCTGTTTGTG
    AATCTGAAAA ATGTGAATCC TGGGAAGAGA GGCCCAGTGG CAGACTGCAC GGGAACATTG
    CCAGCAGACT GGAAACATGG CGGCTACCTT AATGAGCTTG GAGCTTTTGG ACTTGATAGA
    AGTTATATGG GAAAATCCTT GAAAGATTTG GGAAAGAATT CTGAATATAA TTCAAGTAAC
    TGCTCCTTAA GCAGTTCTGA AAACCCATAT GCCACTATTA AAGACCCGCC TGTACTTATC
    CCCAAAAACT CAGAGTGTGG CTATGTGGAA ATGAAGTCAC CAGCACGGAG AGATTCCCCC
    TATGCAGAGA TCAATAACTC AACTTCAGCC AACAAGAATG TCTATGAAGT TGAACCTACA
    GTGAGTGTTG TCCAAGGAAT ATTCAGCAAT AATGGGCATC TCACCCAGGA TCCATACGAC
    CTCCCAAAGA ACAGTCACAT CCCTTGCCAT TATGACCTGC TGCCAGTCCG AGACAGTTCA
    TCCTCCCCCA AGCGAGAGGA TGGTGGCAGT GGCAGCAGCA GCAGCAGTGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe14211
    Clone ID Related Accession (Same CDS sequence) XM_014117692.1 , XM_014117690.2 , XM_005626476.2 , XM_531893.6 , XM_014117690.1 , XM_014117691.1 , XM_005626476.3 , XM_531893.5 , XM_014117691.2
    Accession Version XM_014117690.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3414bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product multiple epidermal growth factor-like domains protein 10 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014117690.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGTTGCTT CTTTGAACTC ATGCCTGAGC TTTATCTGTT TCTCATTATG CCTCTGGAGC 
    AGGACAGCAT CAGCACTGAA TCTTGAAGAC CCTAATGTGT GTAGCCACTG GGAAAGCTAC
    TCAGTGACTG TGCAAGAGTC ATACCCACAT CCTTTCGATC AAATCTACTA CACCAGCTGC
    ACTGATATTC TGAACTGGTT TAAATGTACA CGGCACAGAA TCAGTTATCG GACTGCCTAT
    CGACATGGGG AGAAAACTAT GTATAGGCGC AAATCCCAGT GTTGTCCTGG ATTTTATGAA
    AGCAGGGAAA TGTGTGTCCC CCACTGTGCT GATAAATGTG TCCATGGTCG CTGCATTGCT
    CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGTGGAACCA ACTGCTCCAG TGCCTGTGAT
    GGTGATCACT GGGGGCCCCA TTGCAGCAGC CGGTGCCAGT GCAAGAACGG GGCCCTGTGC
    AACCCCATCA CGGGGGCTTG CCACTGCGCT GCAGGTTTCC GGGGCTGGCG TTGTGAGGAC
    CGCTGTGAGC AGGGCACCTA CGGTAATGAC TGTCACCAGA GATGCCAGTG TCAGAATGGA
    GCCACCTGCG ACCACGTCAC AGGGGAATGC CGCTGCCCAC CAGGGTACAC TGGAGCCTTC
    TGTGAGGACC TTTGTCCCCC TGGCAAGCAC GGTCCGCAGT GCGAGCAGAG ATGTCCCTGC
    CAAAATGGAG GAATATGTCA TCACGTCACC GGAGAATGCT CTTGCCCTTC GGGCTGGCTG
    GGCACAGTCT GTGGTCAGCC TTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCACAAGAA
    TGTCAGTGCC ATAATGGAGG GACGTGTGAT GCTGCCACAG GCCAATGTCA TTGCAGTCCA
    GGATACACAG GGGAACGCTG CCAGGACGAG TGTCCCGTTG GCACATACGG AGTTCGCTGT
    GCTGAGACCT GCCAGTGTGT CAATGGAGGG AAGTGTTATC ACGTGAGTGG CTCATGCCTC
    TGTGAAGCAG GCTTTGCTGG TGAGCACTGT GAGGCACGCC TGTGTCCCGA AGGGCTCTAC
    GGCATCAAAT GTGACAAGCG GTGTCCCTGC CACTTGGACA ACACTCATAG CTGTCACCCC
    ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC TGGTCAGGAC TCTACTGTAA CGAAACATGT
    TCTCCTGGAT TCTTTGGGGA AGCTTGCCAA CAGATCTGCA GCTGCCACAA CGGGGCTGAT
    TGTGACAGTG TGACTGGAAA ATGCATCTGT GCCCCAGGAT TCAAAGGAAT TGACTGCTCT
    ATCCCATGCC CATTGGAAAC CTATGGGATA AACTGCTCCT CTCTCTGTGG CTGTAAAAAC
    GATGCTGTCT GTTCACCTGT AGATGGGTCT TGTACTTGCA AGGCAGGCTG GCACGGGGTG
    GACTGCTCCA TCAGCTGTCC CAGTGGCACG TGGGGCTTCA GCTGTAACCT GACCTGCCAG
    TGCCTCCACG GGGGAGCCTG CAACACCCAG GACGGGACCT GCACGTGTGC GCCTGGATGG
    CGCGGGGACA AATGTGAACT CCCCTGCCAG GATGGCACAT ATGGGCTGAA CTGTGCGGAG
    CGCTGTGACT GTAGCCATGC GGATGGCTGC CACTCCACCA CGGGCCACTG CCGCTGCCTC
    CCTGGATGGT CAGGTGTCCA CTGCGACAGC GTGTGTGCTG AGGGGCGCTG GGGGCCCAAC
    TGCTCTCTGC CCTGCTACTG TAAAAATGGG GCTTCATGCT CCCCAGACGA TGGCATCTGT
    GAGTGTGCGC CAGGGTTCCG AGGCACCACC TGTCAGAGAA TCTGCTCCCC TGGTTTTTAT
    GGGCATCGCT GCAGCCAGAC ATGCCCGCAG TGCGTGCATA GCAGTGGGCC CTGCCACCAC
    ATCACAGGCC TGTGTGACTG CTTGCCTGGC TTCACAGGTG CCCTCTGCAA TGAAGTGTGT
    CCCAGTGGCA GATTTGGGAA AAACTGTGCT GGAGTTTGTA CCTGTACAAA CAATGGAACC
    TGTAACCCCA TTGACCGATC TTGTCAGTGT TATCCAGGGT GGATTGGCAG TGACTGCTCG
    CAACCTTGTC CTCCTGCGCA TTGGGGTCCA AACTGCATCC ACACGTGCAA CTGCCACAAT
    GGGGCGTTCT GCAGTGCATA CGACGGGGAA TGTAAATGCA CTCCTGGCTG GACCGGACTC
    TACTGCACTC AGAGATGTCC CCTGGGTTTT TACGGAAAGG ACTGTGCGTT GATATGTCAG
    TGTCAGAATG GAGCTGACTG TGACCACATC TCTGGGCAGT GTACATGCCG CACTGGGTTC
    ATGGGGAAGC ACTGTGAACA GAAATGCCCT GCGGGAACAT ATGGCTATGG CTGTCGCCAG
    ATATGTGACT GTCTGAACAA CTCCACCTGT GACCATATCA CTGGGACTTG TTACTGTAGC
    CCAGGCTGGA AGGGGGCACG GTGTGATCAA GCTGGAGTCA TTATCGTTGG AAATCTGAAC
    AGCTTAAGCA GGACCAGTAC AGCTCTCCCT GCCAATTCCT ACCAGATCGG AGCCATCGTG
    GGCATCATCA TTCTTGTACT GGTTGTTCTC TTCCTCCTGG CGTTGTTCAT TATTTACAGA
    CAGAAGCAAA AGGGAAAGGA ATCAAGCATG CCTGCAGTTA CCTACACCCC TGCCCTGAGG
    GTCATGAATG CAGACTATAC CATTTCAGAA ACCCTTCCCC ACAGCAACAG TGGAAGTGCT
    AACAGCCATT ACTTCACTAA CCCCAGTTAC CACACACTTA CCCAGTGCGC CACATCCCCA
    CATGTCAACA ACAGGGACAG GATGACCATC GCAAAGTCAA AGAACAATCA GCTGTTTGTG
    AATCTGAAAA ATGTGAATCC TGGGAAGAGA GGCCCAGTGG CAGACTGCAC GGGAACATTG
    CCAGCAGACT GGAAACATGG CGGCTACCTT AATGAGCTTG GAGCTTTTGG ACTTGATAGA
    AGTTATATGG GAAAATCCTT GAAAGATTTG GGAAAGAATT CTGAATATAA TTCAAGTAAC
    TGCTCCTTAA GCAGTTCTGA AAACCCATAT GCCACTATTA AAGACCCGCC TGTACTTATC
    CCCAAAAACT CAGAGTGTGG CTATGTGGAA ATGAAGTCAC CAGCACGGAG AGATTCCCCC
    TATGCAGAGA TCAATAACTC AACTTCAGCC AACAAGAATG TCTATGAAGT TGAACCTACA
    GTGAGTGTTG TCCAAGGAAT ATTCAGCAAT AATGGGCATC TCACCCAGGA TCCATACGAC
    CTCCCAAAGA ACAGTCACAT CCCTTGCCAT TATGACCTGC TGCCAGTCCG AGACAGTTCA
    TCCTCCCCCA AGCGAGAGGA TGGTGGCAGT GGCAGCAGCA GCAGCAGTGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973165.1
    CDS147..3560
    Translation

    Target ORF information:

    RefSeq Version XM_014117690.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris multiple EGF like domains 10 (MEGF10), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014117690.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGTTGCTT CTTTGAACTC ATGCCTGAGC TTTATCTGTT TCTCATTATG CCTCTGGAGC 
    AGGACAGCAT CAGCACTGAA TCTTGAAGAC CCTAATGTGT GTAGCCACTG GGAAAGCTAC
    TCAGTGACTG TGCAAGAGTC ATACCCACAT CCTTTCGATC AAATCTACTA CACCAGCTGC
    ACTGATATTC TGAACTGGTT TAAATGTACA CGGCACAGAA TCAGTTATCG GACTGCCTAT
    CGACATGGGG AGAAAACTAT GTATAGGCGC AAATCCCAGT GTTGTCCTGG ATTTTATGAA
    AGCAGGGAAA TGTGTGTCCC CCACTGTGCT GATAAATGTG TCCATGGTCG CTGCATTGCT
    CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGTGGAACCA ACTGCTCCAG TGCCTGTGAT
    GGTGATCACT GGGGGCCCCA TTGCAGCAGC CGGTGCCAGT GCAAGAACGG GGCCCTGTGC
    AACCCCATCA CGGGGGCTTG CCACTGCGCT GCAGGTTTCC GGGGCTGGCG TTGTGAGGAC
    CGCTGTGAGC AGGGCACCTA CGGTAATGAC TGTCACCAGA GATGCCAGTG TCAGAATGGA
    GCCACCTGCG ACCACGTCAC AGGGGAATGC CGCTGCCCAC CAGGGTACAC TGGAGCCTTC
    TGTGAGGACC TTTGTCCCCC TGGCAAGCAC GGTCCGCAGT GCGAGCAGAG ATGTCCCTGC
    CAAAATGGAG GAATATGTCA TCACGTCACC GGAGAATGCT CTTGCCCTTC GGGCTGGCTG
    GGCACAGTCT GTGGTCAGCC TTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCACAAGAA
    TGTCAGTGCC ATAATGGAGG GACGTGTGAT GCTGCCACAG GCCAATGTCA TTGCAGTCCA
    GGATACACAG GGGAACGCTG CCAGGACGAG TGTCCCGTTG GCACATACGG AGTTCGCTGT
    GCTGAGACCT GCCAGTGTGT CAATGGAGGG AAGTGTTATC ACGTGAGTGG CTCATGCCTC
    TGTGAAGCAG GCTTTGCTGG TGAGCACTGT GAGGCACGCC TGTGTCCCGA AGGGCTCTAC
    GGCATCAAAT GTGACAAGCG GTGTCCCTGC CACTTGGACA ACACTCATAG CTGTCACCCC
    ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC TGGTCAGGAC TCTACTGTAA CGAAACATGT
    TCTCCTGGAT TCTTTGGGGA AGCTTGCCAA CAGATCTGCA GCTGCCACAA CGGGGCTGAT
    TGTGACAGTG TGACTGGAAA ATGCATCTGT GCCCCAGGAT TCAAAGGAAT TGACTGCTCT
    ATCCCATGCC CATTGGAAAC CTATGGGATA AACTGCTCCT CTCTCTGTGG CTGTAAAAAC
    GATGCTGTCT GTTCACCTGT AGATGGGTCT TGTACTTGCA AGGCAGGCTG GCACGGGGTG
    GACTGCTCCA TCAGCTGTCC CAGTGGCACG TGGGGCTTCA GCTGTAACCT GACCTGCCAG
    TGCCTCCACG GGGGAGCCTG CAACACCCAG GACGGGACCT GCACGTGTGC GCCTGGATGG
    CGCGGGGACA AATGTGAACT CCCCTGCCAG GATGGCACAT ATGGGCTGAA CTGTGCGGAG
    CGCTGTGACT GTAGCCATGC GGATGGCTGC CACTCCACCA CGGGCCACTG CCGCTGCCTC
    CCTGGATGGT CAGGTGTCCA CTGCGACAGC GTGTGTGCTG AGGGGCGCTG GGGGCCCAAC
    TGCTCTCTGC CCTGCTACTG TAAAAATGGG GCTTCATGCT CCCCAGACGA TGGCATCTGT
    GAGTGTGCGC CAGGGTTCCG AGGCACCACC TGTCAGAGAA TCTGCTCCCC TGGTTTTTAT
    GGGCATCGCT GCAGCCAGAC ATGCCCGCAG TGCGTGCATA GCAGTGGGCC CTGCCACCAC
    ATCACAGGCC TGTGTGACTG CTTGCCTGGC TTCACAGGTG CCCTCTGCAA TGAAGTGTGT
    CCCAGTGGCA GATTTGGGAA AAACTGTGCT GGAGTTTGTA CCTGTACAAA CAATGGAACC
    TGTAACCCCA TTGACCGATC TTGTCAGTGT TATCCAGGGT GGATTGGCAG TGACTGCTCG
    CAACCTTGTC CTCCTGCGCA TTGGGGTCCA AACTGCATCC ACACGTGCAA CTGCCACAAT
    GGGGCGTTCT GCAGTGCATA CGACGGGGAA TGTAAATGCA CTCCTGGCTG GACCGGACTC
    TACTGCACTC AGAGATGTCC CCTGGGTTTT TACGGAAAGG ACTGTGCGTT GATATGTCAG
    TGTCAGAATG GAGCTGACTG TGACCACATC TCTGGGCAGT GTACATGCCG CACTGGGTTC
    ATGGGGAAGC ACTGTGAACA GAAATGCCCT GCGGGAACAT ATGGCTATGG CTGTCGCCAG
    ATATGTGACT GTCTGAACAA CTCCACCTGT GACCATATCA CTGGGACTTG TTACTGTAGC
    CCAGGCTGGA AGGGGGCACG GTGTGATCAA GCTGGAGTCA TTATCGTTGG AAATCTGAAC
    AGCTTAAGCA GGACCAGTAC AGCTCTCCCT GCCAATTCCT ACCAGATCGG AGCCATCGTG
    GGCATCATCA TTCTTGTACT GGTTGTTCTC TTCCTCCTGG CGTTGTTCAT TATTTACAGA
    CAGAAGCAAA AGGGAAAGGA ATCAAGCATG CCTGCAGTTA CCTACACCCC TGCCCTGAGG
    GTCATGAATG CAGACTATAC CATTTCAGAA ACCCTTCCCC ACAGCAACAG TGGAAGTGCT
    AACAGCCATT ACTTCACTAA CCCCAGTTAC CACACACTTA CCCAGTGCGC CACATCCCCA
    CATGTCAACA ACAGGGACAG GATGACCATC GCAAAGTCAA AGAACAATCA GCTGTTTGTG
    AATCTGAAAA ATGTGAATCC TGGGAAGAGA GGCCCAGTGG CAGACTGCAC GGGAACATTG
    CCAGCAGACT GGAAACATGG CGGCTACCTT AATGAGCTTG GAGCTTTTGG ACTTGATAGA
    AGTTATATGG GAAAATCCTT GAAAGATTTG GGAAAGAATT CTGAATATAA TTCAAGTAAC
    TGCTCCTTAA GCAGTTCTGA AAACCCATAT GCCACTATTA AAGACCCGCC TGTACTTATC
    CCCAAAAACT CAGAGTGTGG CTATGTGGAA ATGAAGTCAC CAGCACGGAG AGATTCCCCC
    TATGCAGAGA TCAATAACTC AACTTCAGCC AACAAGAATG TCTATGAAGT TGAACCTACA
    GTGAGTGTTG TCCAAGGAAT ATTCAGCAAT AATGGGCATC TCACCCAGGA TCCATACGAC
    CTCCCAAAGA ACAGTCACAT CCCTTGCCAT TATGACCTGC TGCCAGTCCG AGACAGTTCA
    TCCTCCCCCA AGCGAGAGGA TGGTGGCAGT GGCAGCAGCA GCAGCAGTGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe14211
    Clone ID Related Accession (Same CDS sequence) XM_014117692.1 , XM_014117690.2 , XM_005626476.2 , XM_531893.6 , XM_014117690.1 , XM_014117691.1 , XM_005626476.3 , XM_531893.5 , XM_014117691.2
    Accession Version XM_005626476.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3414bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product multiple epidermal growth factor-like domains protein 10 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005626476.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGTTGCTT CTTTGAACTC ATGCCTGAGC TTTATCTGTT TCTCATTATG CCTCTGGAGC 
    AGGACAGCAT CAGCACTGAA TCTTGAAGAC CCTAATGTGT GTAGCCACTG GGAAAGCTAC
    TCAGTGACTG TGCAAGAGTC ATACCCACAT CCTTTCGATC AAATCTACTA CACCAGCTGC
    ACTGATATTC TGAACTGGTT TAAATGTACA CGGCACAGAA TCAGTTATCG GACTGCCTAT
    CGACATGGGG AGAAAACTAT GTATAGGCGC AAATCCCAGT GTTGTCCTGG ATTTTATGAA
    AGCAGGGAAA TGTGTGTCCC CCACTGTGCT GATAAATGTG TCCATGGTCG CTGCATTGCT
    CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGTGGAACCA ACTGCTCCAG TGCCTGTGAT
    GGTGATCACT GGGGGCCCCA TTGCAGCAGC CGGTGCCAGT GCAAGAACGG GGCCCTGTGC
    AACCCCATCA CGGGGGCTTG CCACTGCGCT GCAGGTTTCC GGGGCTGGCG TTGTGAGGAC
    CGCTGTGAGC AGGGCACCTA CGGTAATGAC TGTCACCAGA GATGCCAGTG TCAGAATGGA
    GCCACCTGCG ACCACGTCAC AGGGGAATGC CGCTGCCCAC CAGGGTACAC TGGAGCCTTC
    TGTGAGGACC TTTGTCCCCC TGGCAAGCAC GGTCCGCAGT GCGAGCAGAG ATGTCCCTGC
    CAAAATGGAG GAATATGTCA TCACGTCACC GGAGAATGCT CTTGCCCTTC GGGCTGGCTG
    GGCACAGTCT GTGGTCAGCC TTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCACAAGAA
    TGTCAGTGCC ATAATGGAGG GACGTGTGAT GCTGCCACAG GCCAATGTCA TTGCAGTCCA
    GGATACACAG GGGAACGCTG CCAGGACGAG TGTCCCGTTG GCACATACGG AGTTCGCTGT
    GCTGAGACCT GCCAGTGTGT CAATGGAGGG AAGTGTTATC ACGTGAGTGG CTCATGCCTC
    TGTGAAGCAG GCTTTGCTGG TGAGCACTGT GAGGCACGCC TGTGTCCCGA AGGGCTCTAC
    GGCATCAAAT GTGACAAGCG GTGTCCCTGC CACTTGGACA ACACTCATAG CTGTCACCCC
    ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC TGGTCAGGAC TCTACTGTAA CGAAACATGT
    TCTCCTGGAT TCTTTGGGGA AGCTTGCCAA CAGATCTGCA GCTGCCACAA CGGGGCTGAT
    TGTGACAGTG TGACTGGAAA ATGCATCTGT GCCCCAGGAT TCAAAGGAAT TGACTGCTCT
    ATCCCATGCC CATTGGAAAC CTATGGGATA AACTGCTCCT CTCTCTGTGG CTGTAAAAAC
    GATGCTGTCT GTTCACCTGT AGATGGGTCT TGTACTTGCA AGGCAGGCTG GCACGGGGTG
    GACTGCTCCA TCAGCTGTCC CAGTGGCACG TGGGGCTTCA GCTGTAACCT GACCTGCCAG
    TGCCTCCACG GGGGAGCCTG CAACACCCAG GACGGGACCT GCACGTGTGC GCCTGGATGG
    CGCGGGGACA AATGTGAACT CCCCTGCCAG GATGGCACAT ATGGGCTGAA CTGTGCGGAG
    CGCTGTGACT GTAGCCATGC GGATGGCTGC CACTCCACCA CGGGCCACTG CCGCTGCCTC
    CCTGGATGGT CAGGTGTCCA CTGCGACAGC GTGTGTGCTG AGGGGCGCTG GGGGCCCAAC
    TGCTCTCTGC CCTGCTACTG TAAAAATGGG GCTTCATGCT CCCCAGACGA TGGCATCTGT
    GAGTGTGCGC CAGGGTTCCG AGGCACCACC TGTCAGAGAA TCTGCTCCCC TGGTTTTTAT
    GGGCATCGCT GCAGCCAGAC ATGCCCGCAG TGCGTGCATA GCAGTGGGCC CTGCCACCAC
    ATCACAGGCC TGTGTGACTG CTTGCCTGGC TTCACAGGTG CCCTCTGCAA TGAAGTGTGT
    CCCAGTGGCA GATTTGGGAA AAACTGTGCT GGAGTTTGTA CCTGTACAAA CAATGGAACC
    TGTAACCCCA TTGACCGATC TTGTCAGTGT TATCCAGGGT GGATTGGCAG TGACTGCTCG
    CAACCTTGTC CTCCTGCGCA TTGGGGTCCA AACTGCATCC ACACGTGCAA CTGCCACAAT
    GGGGCGTTCT GCAGTGCATA CGACGGGGAA TGTAAATGCA CTCCTGGCTG GACCGGACTC
    TACTGCACTC AGAGATGTCC CCTGGGTTTT TACGGAAAGG ACTGTGCGTT GATATGTCAG
    TGTCAGAATG GAGCTGACTG TGACCACATC TCTGGGCAGT GTACATGCCG CACTGGGTTC
    ATGGGGAAGC ACTGTGAACA GAAATGCCCT GCGGGAACAT ATGGCTATGG CTGTCGCCAG
    ATATGTGACT GTCTGAACAA CTCCACCTGT GACCATATCA CTGGGACTTG TTACTGTAGC
    CCAGGCTGGA AGGGGGCACG GTGTGATCAA GCTGGAGTCA TTATCGTTGG AAATCTGAAC
    AGCTTAAGCA GGACCAGTAC AGCTCTCCCT GCCAATTCCT ACCAGATCGG AGCCATCGTG
    GGCATCATCA TTCTTGTACT GGTTGTTCTC TTCCTCCTGG CGTTGTTCAT TATTTACAGA
    CAGAAGCAAA AGGGAAAGGA ATCAAGCATG CCTGCAGTTA CCTACACCCC TGCCCTGAGG
    GTCATGAATG CAGACTATAC CATTTCAGAA ACCCTTCCCC ACAGCAACAG TGGAAGTGCT
    AACAGCCATT ACTTCACTAA CCCCAGTTAC CACACACTTA CCCAGTGCGC CACATCCCCA
    CATGTCAACA ACAGGGACAG GATGACCATC GCAAAGTCAA AGAACAATCA GCTGTTTGTG
    AATCTGAAAA ATGTGAATCC TGGGAAGAGA GGCCCAGTGG CAGACTGCAC GGGAACATTG
    CCAGCAGACT GGAAACATGG CGGCTACCTT AATGAGCTTG GAGCTTTTGG ACTTGATAGA
    AGTTATATGG GAAAATCCTT GAAAGATTTG GGAAAGAATT CTGAATATAA TTCAAGTAAC
    TGCTCCTTAA GCAGTTCTGA AAACCCATAT GCCACTATTA AAGACCCGCC TGTACTTATC
    CCCAAAAACT CAGAGTGTGG CTATGTGGAA ATGAAGTCAC CAGCACGGAG AGATTCCCCC
    TATGCAGAGA TCAATAACTC AACTTCAGCC AACAAGAATG TCTATGAAGT TGAACCTACA
    GTGAGTGTTG TCCAAGGAAT ATTCAGCAAT AATGGGCATC TCACCCAGGA TCCATACGAC
    CTCCCAAAGA ACAGTCACAT CCCTTGCCAT TATGACCTGC TGCCAGTCCG AGACAGTTCA
    TCCTCCCCCA AGCGAGAGGA TGGTGGCAGT GGCAGCAGCA GCAGCAGTGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005626533.1
    CDS800..4213
    Translation

    Target ORF information:

    RefSeq Version XM_005626476.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris multiple EGF-like-domains 10 (MEGF10), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005626476.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGTTGCTT CTTTGAACTC ATGCCTGAGC TTTATCTGTT TCTCATTATG CCTCTGGAGC 
    AGGACAGCAT CAGCACTGAA TCTTGAAGAC CCTAATGTGT GTAGCCACTG GGAAAGCTAC
    TCAGTGACTG TGCAAGAGTC ATACCCACAT CCTTTCGATC AAATCTACTA CACCAGCTGC
    ACTGATATTC TGAACTGGTT TAAATGTACA CGGCACAGAA TCAGTTATCG GACTGCCTAT
    CGACATGGGG AGAAAACTAT GTATAGGCGC AAATCCCAGT GTTGTCCTGG ATTTTATGAA
    AGCAGGGAAA TGTGTGTCCC CCACTGTGCT GATAAATGTG TCCATGGTCG CTGCATTGCT
    CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGTGGAACCA ACTGCTCCAG TGCCTGTGAT
    GGTGATCACT GGGGGCCCCA TTGCAGCAGC CGGTGCCAGT GCAAGAACGG GGCCCTGTGC
    AACCCCATCA CGGGGGCTTG CCACTGCGCT GCAGGTTTCC GGGGCTGGCG TTGTGAGGAC
    CGCTGTGAGC AGGGCACCTA CGGTAATGAC TGTCACCAGA GATGCCAGTG TCAGAATGGA
    GCCACCTGCG ACCACGTCAC AGGGGAATGC CGCTGCCCAC CAGGGTACAC TGGAGCCTTC
    TGTGAGGACC TTTGTCCCCC TGGCAAGCAC GGTCCGCAGT GCGAGCAGAG ATGTCCCTGC
    CAAAATGGAG GAATATGTCA TCACGTCACC GGAGAATGCT CTTGCCCTTC GGGCTGGCTG
    GGCACAGTCT GTGGTCAGCC TTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCACAAGAA
    TGTCAGTGCC ATAATGGAGG GACGTGTGAT GCTGCCACAG GCCAATGTCA TTGCAGTCCA
    GGATACACAG GGGAACGCTG CCAGGACGAG TGTCCCGTTG GCACATACGG AGTTCGCTGT
    GCTGAGACCT GCCAGTGTGT CAATGGAGGG AAGTGTTATC ACGTGAGTGG CTCATGCCTC
    TGTGAAGCAG GCTTTGCTGG TGAGCACTGT GAGGCACGCC TGTGTCCCGA AGGGCTCTAC
    GGCATCAAAT GTGACAAGCG GTGTCCCTGC CACTTGGACA ACACTCATAG CTGTCACCCC
    ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC TGGTCAGGAC TCTACTGTAA CGAAACATGT
    TCTCCTGGAT TCTTTGGGGA AGCTTGCCAA CAGATCTGCA GCTGCCACAA CGGGGCTGAT
    TGTGACAGTG TGACTGGAAA ATGCATCTGT GCCCCAGGAT TCAAAGGAAT TGACTGCTCT
    ATCCCATGCC CATTGGAAAC CTATGGGATA AACTGCTCCT CTCTCTGTGG CTGTAAAAAC
    GATGCTGTCT GTTCACCTGT AGATGGGTCT TGTACTTGCA AGGCAGGCTG GCACGGGGTG
    GACTGCTCCA TCAGCTGTCC CAGTGGCACG TGGGGCTTCA GCTGTAACCT GACCTGCCAG
    TGCCTCCACG GGGGAGCCTG CAACACCCAG GACGGGACCT GCACGTGTGC GCCTGGATGG
    CGCGGGGACA AATGTGAACT CCCCTGCCAG GATGGCACAT ATGGGCTGAA CTGTGCGGAG
    CGCTGTGACT GTAGCCATGC GGATGGCTGC CACTCCACCA CGGGCCACTG CCGCTGCCTC
    CCTGGATGGT CAGGTGTCCA CTGCGACAGC GTGTGTGCTG AGGGGCGCTG GGGGCCCAAC
    TGCTCTCTGC CCTGCTACTG TAAAAATGGG GCTTCATGCT CCCCAGACGA TGGCATCTGT
    GAGTGTGCGC CAGGGTTCCG AGGCACCACC TGTCAGAGAA TCTGCTCCCC TGGTTTTTAT
    GGGCATCGCT GCAGCCAGAC ATGCCCGCAG TGCGTGCATA GCAGTGGGCC CTGCCACCAC
    ATCACAGGCC TGTGTGACTG CTTGCCTGGC TTCACAGGTG CCCTCTGCAA TGAAGTGTGT
    CCCAGTGGCA GATTTGGGAA AAACTGTGCT GGAGTTTGTA CCTGTACAAA CAATGGAACC
    TGTAACCCCA TTGACCGATC TTGTCAGTGT TATCCAGGGT GGATTGGCAG TGACTGCTCG
    CAACCTTGTC CTCCTGCGCA TTGGGGTCCA AACTGCATCC ACACGTGCAA CTGCCACAAT
    GGGGCGTTCT GCAGTGCATA CGACGGGGAA TGTAAATGCA CTCCTGGCTG GACCGGACTC
    TACTGCACTC AGAGATGTCC CCTGGGTTTT TACGGAAAGG ACTGTGCGTT GATATGTCAG
    TGTCAGAATG GAGCTGACTG TGACCACATC TCTGGGCAGT GTACATGCCG CACTGGGTTC
    ATGGGGAAGC ACTGTGAACA GAAATGCCCT GCGGGAACAT ATGGCTATGG CTGTCGCCAG
    ATATGTGACT GTCTGAACAA CTCCACCTGT GACCATATCA CTGGGACTTG TTACTGTAGC
    CCAGGCTGGA AGGGGGCACG GTGTGATCAA GCTGGAGTCA TTATCGTTGG AAATCTGAAC
    AGCTTAAGCA GGACCAGTAC AGCTCTCCCT GCCAATTCCT ACCAGATCGG AGCCATCGTG
    GGCATCATCA TTCTTGTACT GGTTGTTCTC TTCCTCCTGG CGTTGTTCAT TATTTACAGA
    CAGAAGCAAA AGGGAAAGGA ATCAAGCATG CCTGCAGTTA CCTACACCCC TGCCCTGAGG
    GTCATGAATG CAGACTATAC CATTTCAGAA ACCCTTCCCC ACAGCAACAG TGGAAGTGCT
    AACAGCCATT ACTTCACTAA CCCCAGTTAC CACACACTTA CCCAGTGCGC CACATCCCCA
    CATGTCAACA ACAGGGACAG GATGACCATC GCAAAGTCAA AGAACAATCA GCTGTTTGTG
    AATCTGAAAA ATGTGAATCC TGGGAAGAGA GGCCCAGTGG CAGACTGCAC GGGAACATTG
    CCAGCAGACT GGAAACATGG CGGCTACCTT AATGAGCTTG GAGCTTTTGG ACTTGATAGA
    AGTTATATGG GAAAATCCTT GAAAGATTTG GGAAAGAATT CTGAATATAA TTCAAGTAAC
    TGCTCCTTAA GCAGTTCTGA AAACCCATAT GCCACTATTA AAGACCCGCC TGTACTTATC
    CCCAAAAACT CAGAGTGTGG CTATGTGGAA ATGAAGTCAC CAGCACGGAG AGATTCCCCC
    TATGCAGAGA TCAATAACTC AACTTCAGCC AACAAGAATG TCTATGAAGT TGAACCTACA
    GTGAGTGTTG TCCAAGGAAT ATTCAGCAAT AATGGGCATC TCACCCAGGA TCCATACGAC
    CTCCCAAAGA ACAGTCACAT CCCTTGCCAT TATGACCTGC TGCCAGTCCG AGACAGTTCA
    TCCTCCCCCA AGCGAGAGGA TGGTGGCAGT GGCAGCAGCA GCAGCAGTGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe14211
    Clone ID Related Accession (Same CDS sequence) XM_014117692.1 , XM_014117690.2 , XM_005626476.2 , XM_531893.6 , XM_014117690.1 , XM_014117691.1 , XM_005626476.3 , XM_531893.5 , XM_014117691.2
    Accession Version XM_531893.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3414bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product multiple epidermal growth factor-like domains protein 10 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_531893.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGTTGCTT CTTTGAACTC ATGCCTGAGC TTTATCTGTT TCTCATTATG CCTCTGGAGC 
    AGGACAGCAT CAGCACTGAA TCTTGAAGAC CCTAATGTGT GTAGCCACTG GGAAAGCTAC
    TCAGTGACTG TGCAAGAGTC ATACCCACAT CCTTTCGATC AAATCTACTA CACCAGCTGC
    ACTGATATTC TGAACTGGTT TAAATGTACA CGGCACAGAA TCAGTTATCG GACTGCCTAT
    CGACATGGGG AGAAAACTAT GTATAGGCGC AAATCCCAGT GTTGTCCTGG ATTTTATGAA
    AGCAGGGAAA TGTGTGTCCC CCACTGTGCT GATAAATGTG TCCATGGTCG CTGCATTGCT
    CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGTGGAACCA ACTGCTCCAG TGCCTGTGAT
    GGTGATCACT GGGGGCCCCA TTGCAGCAGC CGGTGCCAGT GCAAGAACGG GGCCCTGTGC
    AACCCCATCA CGGGGGCTTG CCACTGCGCT GCAGGTTTCC GGGGCTGGCG TTGTGAGGAC
    CGCTGTGAGC AGGGCACCTA CGGTAATGAC TGTCACCAGA GATGCCAGTG TCAGAATGGA
    GCCACCTGCG ACCACGTCAC AGGGGAATGC CGCTGCCCAC CAGGGTACAC TGGAGCCTTC
    TGTGAGGACC TTTGTCCCCC TGGCAAGCAC GGTCCGCAGT GCGAGCAGAG ATGTCCCTGC
    CAAAATGGAG GAATATGTCA TCACGTCACC GGAGAATGCT CTTGCCCTTC GGGCTGGCTG
    GGCACAGTCT GTGGTCAGCC TTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCACAAGAA
    TGTCAGTGCC ATAATGGAGG GACGTGTGAT GCTGCCACAG GCCAATGTCA TTGCAGTCCA
    GGATACACAG GGGAACGCTG CCAGGACGAG TGTCCCGTTG GCACATACGG AGTTCGCTGT
    GCTGAGACCT GCCAGTGTGT CAATGGAGGG AAGTGTTATC ACGTGAGTGG CTCATGCCTC
    TGTGAAGCAG GCTTTGCTGG TGAGCACTGT GAGGCACGCC TGTGTCCCGA AGGGCTCTAC
    GGCATCAAAT GTGACAAGCG GTGTCCCTGC CACTTGGACA ACACTCATAG CTGTCACCCC
    ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC TGGTCAGGAC TCTACTGTAA CGAAACATGT
    TCTCCTGGAT TCTTTGGGGA AGCTTGCCAA CAGATCTGCA GCTGCCACAA CGGGGCTGAT
    TGTGACAGTG TGACTGGAAA ATGCATCTGT GCCCCAGGAT TCAAAGGAAT TGACTGCTCT
    ATCCCATGCC CATTGGAAAC CTATGGGATA AACTGCTCCT CTCTCTGTGG CTGTAAAAAC
    GATGCTGTCT GTTCACCTGT AGATGGGTCT TGTACTTGCA AGGCAGGCTG GCACGGGGTG
    GACTGCTCCA TCAGCTGTCC CAGTGGCACG TGGGGCTTCA GCTGTAACCT GACCTGCCAG
    TGCCTCCACG GGGGAGCCTG CAACACCCAG GACGGGACCT GCACGTGTGC GCCTGGATGG
    CGCGGGGACA AATGTGAACT CCCCTGCCAG GATGGCACAT ATGGGCTGAA CTGTGCGGAG
    CGCTGTGACT GTAGCCATGC GGATGGCTGC CACTCCACCA CGGGCCACTG CCGCTGCCTC
    CCTGGATGGT CAGGTGTCCA CTGCGACAGC GTGTGTGCTG AGGGGCGCTG GGGGCCCAAC
    TGCTCTCTGC CCTGCTACTG TAAAAATGGG GCTTCATGCT CCCCAGACGA TGGCATCTGT
    GAGTGTGCGC CAGGGTTCCG AGGCACCACC TGTCAGAGAA TCTGCTCCCC TGGTTTTTAT
    GGGCATCGCT GCAGCCAGAC ATGCCCGCAG TGCGTGCATA GCAGTGGGCC CTGCCACCAC
    ATCACAGGCC TGTGTGACTG CTTGCCTGGC TTCACAGGTG CCCTCTGCAA TGAAGTGTGT
    CCCAGTGGCA GATTTGGGAA AAACTGTGCT GGAGTTTGTA CCTGTACAAA CAATGGAACC
    TGTAACCCCA TTGACCGATC TTGTCAGTGT TATCCAGGGT GGATTGGCAG TGACTGCTCG
    CAACCTTGTC CTCCTGCGCA TTGGGGTCCA AACTGCATCC ACACGTGCAA CTGCCACAAT
    GGGGCGTTCT GCAGTGCATA CGACGGGGAA TGTAAATGCA CTCCTGGCTG GACCGGACTC
    TACTGCACTC AGAGATGTCC CCTGGGTTTT TACGGAAAGG ACTGTGCGTT GATATGTCAG
    TGTCAGAATG GAGCTGACTG TGACCACATC TCTGGGCAGT GTACATGCCG CACTGGGTTC
    ATGGGGAAGC ACTGTGAACA GAAATGCCCT GCGGGAACAT ATGGCTATGG CTGTCGCCAG
    ATATGTGACT GTCTGAACAA CTCCACCTGT GACCATATCA CTGGGACTTG TTACTGTAGC
    CCAGGCTGGA AGGGGGCACG GTGTGATCAA GCTGGAGTCA TTATCGTTGG AAATCTGAAC
    AGCTTAAGCA GGACCAGTAC AGCTCTCCCT GCCAATTCCT ACCAGATCGG AGCCATCGTG
    GGCATCATCA TTCTTGTACT GGTTGTTCTC TTCCTCCTGG CGTTGTTCAT TATTTACAGA
    CAGAAGCAAA AGGGAAAGGA ATCAAGCATG CCTGCAGTTA CCTACACCCC TGCCCTGAGG
    GTCATGAATG CAGACTATAC CATTTCAGAA ACCCTTCCCC ACAGCAACAG TGGAAGTGCT
    AACAGCCATT ACTTCACTAA CCCCAGTTAC CACACACTTA CCCAGTGCGC CACATCCCCA
    CATGTCAACA ACAGGGACAG GATGACCATC GCAAAGTCAA AGAACAATCA GCTGTTTGTG
    AATCTGAAAA ATGTGAATCC TGGGAAGAGA GGCCCAGTGG CAGACTGCAC GGGAACATTG
    CCAGCAGACT GGAAACATGG CGGCTACCTT AATGAGCTTG GAGCTTTTGG ACTTGATAGA
    AGTTATATGG GAAAATCCTT GAAAGATTTG GGAAAGAATT CTGAATATAA TTCAAGTAAC
    TGCTCCTTAA GCAGTTCTGA AAACCCATAT GCCACTATTA AAGACCCGCC TGTACTTATC
    CCCAAAAACT CAGAGTGTGG CTATGTGGAA ATGAAGTCAC CAGCACGGAG AGATTCCCCC
    TATGCAGAGA TCAATAACTC AACTTCAGCC AACAAGAATG TCTATGAAGT TGAACCTACA
    GTGAGTGTTG TCCAAGGAAT ATTCAGCAAT AATGGGCATC TCACCCAGGA TCCATACGAC
    CTCCCAAAGA ACAGTCACAT CCCTTGCCAT TATGACCTGC TGCCAGTCCG AGACAGTTCA
    TCCTCCCCCA AGCGAGAGGA TGGTGGCAGT GGCAGCAGCA GCAGCAGTGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_531893.3
    CDS246..3659
    Translation

    Target ORF information:

    RefSeq Version XM_531893.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris multiple EGF like domains 10 (MEGF10), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_531893.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGTTGCTT CTTTGAACTC ATGCCTGAGC TTTATCTGTT TCTCATTATG CCTCTGGAGC 
    AGGACAGCAT CAGCACTGAA TCTTGAAGAC CCTAATGTGT GTAGCCACTG GGAAAGCTAC
    TCAGTGACTG TGCAAGAGTC ATACCCACAT CCTTTCGATC AAATCTACTA CACCAGCTGC
    ACTGATATTC TGAACTGGTT TAAATGTACA CGGCACAGAA TCAGTTATCG GACTGCCTAT
    CGACATGGGG AGAAAACTAT GTATAGGCGC AAATCCCAGT GTTGTCCTGG ATTTTATGAA
    AGCAGGGAAA TGTGTGTCCC CCACTGTGCT GATAAATGTG TCCATGGTCG CTGCATTGCT
    CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGTGGAACCA ACTGCTCCAG TGCCTGTGAT
    GGTGATCACT GGGGGCCCCA TTGCAGCAGC CGGTGCCAGT GCAAGAACGG GGCCCTGTGC
    AACCCCATCA CGGGGGCTTG CCACTGCGCT GCAGGTTTCC GGGGCTGGCG TTGTGAGGAC
    CGCTGTGAGC AGGGCACCTA CGGTAATGAC TGTCACCAGA GATGCCAGTG TCAGAATGGA
    GCCACCTGCG ACCACGTCAC AGGGGAATGC CGCTGCCCAC CAGGGTACAC TGGAGCCTTC
    TGTGAGGACC TTTGTCCCCC TGGCAAGCAC GGTCCGCAGT GCGAGCAGAG ATGTCCCTGC
    CAAAATGGAG GAATATGTCA TCACGTCACC GGAGAATGCT CTTGCCCTTC GGGCTGGCTG
    GGCACAGTCT GTGGTCAGCC TTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCACAAGAA
    TGTCAGTGCC ATAATGGAGG GACGTGTGAT GCTGCCACAG GCCAATGTCA TTGCAGTCCA
    GGATACACAG GGGAACGCTG CCAGGACGAG TGTCCCGTTG GCACATACGG AGTTCGCTGT
    GCTGAGACCT GCCAGTGTGT CAATGGAGGG AAGTGTTATC ACGTGAGTGG CTCATGCCTC
    TGTGAAGCAG GCTTTGCTGG TGAGCACTGT GAGGCACGCC TGTGTCCCGA AGGGCTCTAC
    GGCATCAAAT GTGACAAGCG GTGTCCCTGC CACTTGGACA ACACTCATAG CTGTCACCCC
    ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC TGGTCAGGAC TCTACTGTAA CGAAACATGT
    TCTCCTGGAT TCTTTGGGGA AGCTTGCCAA CAGATCTGCA GCTGCCACAA CGGGGCTGAT
    TGTGACAGTG TGACTGGAAA ATGCATCTGT GCCCCAGGAT TCAAAGGAAT TGACTGCTCT
    ATCCCATGCC CATTGGAAAC CTATGGGATA AACTGCTCCT CTCTCTGTGG CTGTAAAAAC
    GATGCTGTCT GTTCACCTGT AGATGGGTCT TGTACTTGCA AGGCAGGCTG GCACGGGGTG
    GACTGCTCCA TCAGCTGTCC CAGTGGCACG TGGGGCTTCA GCTGTAACCT GACCTGCCAG
    TGCCTCCACG GGGGAGCCTG CAACACCCAG GACGGGACCT GCACGTGTGC GCCTGGATGG
    CGCGGGGACA AATGTGAACT CCCCTGCCAG GATGGCACAT ATGGGCTGAA CTGTGCGGAG
    CGCTGTGACT GTAGCCATGC GGATGGCTGC CACTCCACCA CGGGCCACTG CCGCTGCCTC
    CCTGGATGGT CAGGTGTCCA CTGCGACAGC GTGTGTGCTG AGGGGCGCTG GGGGCCCAAC
    TGCTCTCTGC CCTGCTACTG TAAAAATGGG GCTTCATGCT CCCCAGACGA TGGCATCTGT
    GAGTGTGCGC CAGGGTTCCG AGGCACCACC TGTCAGAGAA TCTGCTCCCC TGGTTTTTAT
    GGGCATCGCT GCAGCCAGAC ATGCCCGCAG TGCGTGCATA GCAGTGGGCC CTGCCACCAC
    ATCACAGGCC TGTGTGACTG CTTGCCTGGC TTCACAGGTG CCCTCTGCAA TGAAGTGTGT
    CCCAGTGGCA GATTTGGGAA AAACTGTGCT GGAGTTTGTA CCTGTACAAA CAATGGAACC
    TGTAACCCCA TTGACCGATC TTGTCAGTGT TATCCAGGGT GGATTGGCAG TGACTGCTCG
    CAACCTTGTC CTCCTGCGCA TTGGGGTCCA AACTGCATCC ACACGTGCAA CTGCCACAAT
    GGGGCGTTCT GCAGTGCATA CGACGGGGAA TGTAAATGCA CTCCTGGCTG GACCGGACTC
    TACTGCACTC AGAGATGTCC CCTGGGTTTT TACGGAAAGG ACTGTGCGTT GATATGTCAG
    TGTCAGAATG GAGCTGACTG TGACCACATC TCTGGGCAGT GTACATGCCG CACTGGGTTC
    ATGGGGAAGC ACTGTGAACA GAAATGCCCT GCGGGAACAT ATGGCTATGG CTGTCGCCAG
    ATATGTGACT GTCTGAACAA CTCCACCTGT GACCATATCA CTGGGACTTG TTACTGTAGC
    CCAGGCTGGA AGGGGGCACG GTGTGATCAA GCTGGAGTCA TTATCGTTGG AAATCTGAAC
    AGCTTAAGCA GGACCAGTAC AGCTCTCCCT GCCAATTCCT ACCAGATCGG AGCCATCGTG
    GGCATCATCA TTCTTGTACT GGTTGTTCTC TTCCTCCTGG CGTTGTTCAT TATTTACAGA
    CAGAAGCAAA AGGGAAAGGA ATCAAGCATG CCTGCAGTTA CCTACACCCC TGCCCTGAGG
    GTCATGAATG CAGACTATAC CATTTCAGAA ACCCTTCCCC ACAGCAACAG TGGAAGTGCT
    AACAGCCATT ACTTCACTAA CCCCAGTTAC CACACACTTA CCCAGTGCGC CACATCCCCA
    CATGTCAACA ACAGGGACAG GATGACCATC GCAAAGTCAA AGAACAATCA GCTGTTTGTG
    AATCTGAAAA ATGTGAATCC TGGGAAGAGA GGCCCAGTGG CAGACTGCAC GGGAACATTG
    CCAGCAGACT GGAAACATGG CGGCTACCTT AATGAGCTTG GAGCTTTTGG ACTTGATAGA
    AGTTATATGG GAAAATCCTT GAAAGATTTG GGAAAGAATT CTGAATATAA TTCAAGTAAC
    TGCTCCTTAA GCAGTTCTGA AAACCCATAT GCCACTATTA AAGACCCGCC TGTACTTATC
    CCCAAAAACT CAGAGTGTGG CTATGTGGAA ATGAAGTCAC CAGCACGGAG AGATTCCCCC
    TATGCAGAGA TCAATAACTC AACTTCAGCC AACAAGAATG TCTATGAAGT TGAACCTACA
    GTGAGTGTTG TCCAAGGAAT ATTCAGCAAT AATGGGCATC TCACCCAGGA TCCATACGAC
    CTCCCAAAGA ACAGTCACAT CCCTTGCCAT TATGACCTGC TGCCAGTCCG AGACAGTTCA
    TCCTCCCCCA AGCGAGAGGA TGGTGGCAGT GGCAGCAGCA GCAGCAGTGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe14211
    Clone ID Related Accession (Same CDS sequence) XM_014117692.1 , XM_014117690.2 , XM_005626476.2 , XM_531893.6 , XM_014117690.1 , XM_014117691.1 , XM_005626476.3 , XM_531893.5 , XM_014117691.2
    Accession Version XM_014117690.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3414bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product multiple epidermal growth factor-like domains protein 10 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGTTGCTT CTTTGAACTC ATGCCTGAGC TTTATCTGTT TCTCATTATG CCTCTGGAGC 
    AGGACAGCAT CAGCACTGAA TCTTGAAGAC CCTAATGTGT GTAGCCACTG GGAAAGCTAC
    TCAGTGACTG TGCAAGAGTC ATACCCACAT CCTTTCGATC AAATCTACTA CACCAGCTGC
    ACTGATATTC TGAACTGGTT TAAATGTACA CGGCACAGAA TCAGTTATCG GACTGCCTAT
    CGACATGGGG AGAAAACTAT GTATAGGCGC AAATCCCAGT GTTGTCCTGG ATTTTATGAA
    AGCAGGGAAA TGTGTGTCCC CCACTGTGCT GATAAATGTG TCCATGGTCG CTGCATTGCT
    CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGTGGAACCA ACTGCTCCAG TGCCTGTGAT
    GGTGATCACT GGGGGCCCCA TTGCAGCAGC CGGTGCCAGT GCAAGAACGG GGCCCTGTGC
    AACCCCATCA CGGGGGCTTG CCACTGCGCT GCAGGTTTCC GGGGCTGGCG TTGTGAGGAC
    CGCTGTGAGC AGGGCACCTA CGGTAATGAC TGTCACCAGA GATGCCAGTG TCAGAATGGA
    GCCACCTGCG ACCACGTCAC AGGGGAATGC CGCTGCCCAC CAGGGTACAC TGGAGCCTTC
    TGTGAGGACC TTTGTCCCCC TGGCAAGCAC GGTCCGCAGT GCGAGCAGAG ATGTCCCTGC
    CAAAATGGAG GAATATGTCA TCACGTCACC GGAGAATGCT CTTGCCCTTC GGGCTGGCTG
    GGCACAGTCT GTGGTCAGCC TTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCACAAGAA
    TGTCAGTGCC ATAATGGAGG GACGTGTGAT GCTGCCACAG GCCAATGTCA TTGCAGTCCA
    GGATACACAG GGGAACGCTG CCAGGACGAG TGTCCCGTTG GCACATACGG AGTTCGCTGT
    GCTGAGACCT GCCAGTGTGT CAATGGAGGG AAGTGTTATC ACGTGAGTGG CTCATGCCTC
    TGTGAAGCAG GCTTTGCTGG TGAGCACTGT GAGGCACGCC TGTGTCCCGA AGGGCTCTAC
    GGCATCAAAT GTGACAAGCG GTGTCCCTGC CACTTGGACA ACACTCATAG CTGTCACCCC
    ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC TGGTCAGGAC TCTACTGTAA CGAAACATGT
    TCTCCTGGAT TCTTTGGGGA AGCTTGCCAA CAGATCTGCA GCTGCCACAA CGGGGCTGAT
    TGTGACAGTG TGACTGGAAA ATGCATCTGT GCCCCAGGAT TCAAAGGAAT TGACTGCTCT
    ATCCCATGCC CATTGGAAAC CTATGGGATA AACTGCTCCT CTCTCTGTGG CTGTAAAAAC
    GATGCTGTCT GTTCACCTGT AGATGGGTCT TGTACTTGCA AGGCAGGCTG GCACGGGGTG
    GACTGCTCCA TCAGCTGTCC CAGTGGCACG TGGGGCTTCA GCTGTAACCT GACCTGCCAG
    TGCCTCCACG GGGGAGCCTG CAACACCCAG GACGGGACCT GCACGTGTGC GCCTGGATGG
    CGCGGGGACA AATGTGAACT CCCCTGCCAG GATGGCACAT ATGGGCTGAA CTGTGCGGAG
    CGCTGTGACT GTAGCCATGC GGATGGCTGC CACTCCACCA CGGGCCACTG CCGCTGCCTC
    CCTGGATGGT CAGGTGTCCA CTGCGACAGC GTGTGTGCTG AGGGGCGCTG GGGGCCCAAC
    TGCTCTCTGC CCTGCTACTG TAAAAATGGG GCTTCATGCT CCCCAGACGA TGGCATCTGT
    GAGTGTGCGC CAGGGTTCCG AGGCACCACC TGTCAGAGAA TCTGCTCCCC TGGTTTTTAT
    GGGCATCGCT GCAGCCAGAC ATGCCCGCAG TGCGTGCATA GCAGTGGGCC CTGCCACCAC
    ATCACAGGCC TGTGTGACTG CTTGCCTGGC TTCACAGGTG CCCTCTGCAA TGAAGTGTGT
    CCCAGTGGCA GATTTGGGAA AAACTGTGCT GGAGTTTGTA CCTGTACAAA CAATGGAACC
    TGTAACCCCA TTGACCGATC TTGTCAGTGT TATCCAGGGT GGATTGGCAG TGACTGCTCG
    CAACCTTGTC CTCCTGCGCA TTGGGGTCCA AACTGCATCC ACACGTGCAA CTGCCACAAT
    GGGGCGTTCT GCAGTGCATA CGACGGGGAA TGTAAATGCA CTCCTGGCTG GACCGGACTC
    TACTGCACTC AGAGATGTCC CCTGGGTTTT TACGGAAAGG ACTGTGCGTT GATATGTCAG
    TGTCAGAATG GAGCTGACTG TGACCACATC TCTGGGCAGT GTACATGCCG CACTGGGTTC
    ATGGGGAAGC ACTGTGAACA GAAATGCCCT GCGGGAACAT ATGGCTATGG CTGTCGCCAG
    ATATGTGACT GTCTGAACAA CTCCACCTGT GACCATATCA CTGGGACTTG TTACTGTAGC
    CCAGGCTGGA AGGGGGCACG GTGTGATCAA GCTGGAGTCA TTATCGTTGG AAATCTGAAC
    AGCTTAAGCA GGACCAGTAC AGCTCTCCCT GCCAATTCCT ACCAGATCGG AGCCATCGTG
    GGCATCATCA TTCTTGTACT GGTTGTTCTC TTCCTCCTGG CGTTGTTCAT TATTTACAGA
    CAGAAGCAAA AGGGAAAGGA ATCAAGCATG CCTGCAGTTA CCTACACCCC TGCCCTGAGG
    GTCATGAATG CAGACTATAC CATTTCAGAA ACCCTTCCCC ACAGCAACAG TGGAAGTGCT
    AACAGCCATT ACTTCACTAA CCCCAGTTAC CACACACTTA CCCAGTGCGC CACATCCCCA
    CATGTCAACA ACAGGGACAG GATGACCATC GCAAAGTCAA AGAACAATCA GCTGTTTGTG
    AATCTGAAAA ATGTGAATCC TGGGAAGAGA GGCCCAGTGG CAGACTGCAC GGGAACATTG
    CCAGCAGACT GGAAACATGG CGGCTACCTT AATGAGCTTG GAGCTTTTGG ACTTGATAGA
    AGTTATATGG GAAAATCCTT GAAAGATTTG GGAAAGAATT CTGAATATAA TTCAAGTAAC
    TGCTCCTTAA GCAGTTCTGA AAACCCATAT GCCACTATTA AAGACCCGCC TGTACTTATC
    CCCAAAAACT CAGAGTGTGG CTATGTGGAA ATGAAGTCAC CAGCACGGAG AGATTCCCCC
    TATGCAGAGA TCAATAACTC AACTTCAGCC AACAAGAATG TCTATGAAGT TGAACCTACA
    GTGAGTGTTG TCCAAGGAAT ATTCAGCAAT AATGGGCATC TCACCCAGGA TCCATACGAC
    CTCCCAAAGA ACAGTCACAT CCCTTGCCAT TATGACCTGC TGCCAGTCCG AGACAGTTCA
    TCCTCCCCCA AGCGAGAGGA TGGTGGCAGT GGCAGCAGCA GCAGCAGTGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973165.1
    CDS97..3510
    Translation

    Target ORF information:

    RefSeq Version XM_014117690.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris multiple EGF-like-domains 10 (MEGF10), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014117690.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGTTGCTT CTTTGAACTC ATGCCTGAGC TTTATCTGTT TCTCATTATG CCTCTGGAGC 
    AGGACAGCAT CAGCACTGAA TCTTGAAGAC CCTAATGTGT GTAGCCACTG GGAAAGCTAC
    TCAGTGACTG TGCAAGAGTC ATACCCACAT CCTTTCGATC AAATCTACTA CACCAGCTGC
    ACTGATATTC TGAACTGGTT TAAATGTACA CGGCACAGAA TCAGTTATCG GACTGCCTAT
    CGACATGGGG AGAAAACTAT GTATAGGCGC AAATCCCAGT GTTGTCCTGG ATTTTATGAA
    AGCAGGGAAA TGTGTGTCCC CCACTGTGCT GATAAATGTG TCCATGGTCG CTGCATTGCT
    CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGTGGAACCA ACTGCTCCAG TGCCTGTGAT
    GGTGATCACT GGGGGCCCCA TTGCAGCAGC CGGTGCCAGT GCAAGAACGG GGCCCTGTGC
    AACCCCATCA CGGGGGCTTG CCACTGCGCT GCAGGTTTCC GGGGCTGGCG TTGTGAGGAC
    CGCTGTGAGC AGGGCACCTA CGGTAATGAC TGTCACCAGA GATGCCAGTG TCAGAATGGA
    GCCACCTGCG ACCACGTCAC AGGGGAATGC CGCTGCCCAC CAGGGTACAC TGGAGCCTTC
    TGTGAGGACC TTTGTCCCCC TGGCAAGCAC GGTCCGCAGT GCGAGCAGAG ATGTCCCTGC
    CAAAATGGAG GAATATGTCA TCACGTCACC GGAGAATGCT CTTGCCCTTC GGGCTGGCTG
    GGCACAGTCT GTGGTCAGCC TTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCACAAGAA
    TGTCAGTGCC ATAATGGAGG GACGTGTGAT GCTGCCACAG GCCAATGTCA TTGCAGTCCA
    GGATACACAG GGGAACGCTG CCAGGACGAG TGTCCCGTTG GCACATACGG AGTTCGCTGT
    GCTGAGACCT GCCAGTGTGT CAATGGAGGG AAGTGTTATC ACGTGAGTGG CTCATGCCTC
    TGTGAAGCAG GCTTTGCTGG TGAGCACTGT GAGGCACGCC TGTGTCCCGA AGGGCTCTAC
    GGCATCAAAT GTGACAAGCG GTGTCCCTGC CACTTGGACA ACACTCATAG CTGTCACCCC
    ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC TGGTCAGGAC TCTACTGTAA CGAAACATGT
    TCTCCTGGAT TCTTTGGGGA AGCTTGCCAA CAGATCTGCA GCTGCCACAA CGGGGCTGAT
    TGTGACAGTG TGACTGGAAA ATGCATCTGT GCCCCAGGAT TCAAAGGAAT TGACTGCTCT
    ATCCCATGCC CATTGGAAAC CTATGGGATA AACTGCTCCT CTCTCTGTGG CTGTAAAAAC
    GATGCTGTCT GTTCACCTGT AGATGGGTCT TGTACTTGCA AGGCAGGCTG GCACGGGGTG
    GACTGCTCCA TCAGCTGTCC CAGTGGCACG TGGGGCTTCA GCTGTAACCT GACCTGCCAG
    TGCCTCCACG GGGGAGCCTG CAACACCCAG GACGGGACCT GCACGTGTGC GCCTGGATGG
    CGCGGGGACA AATGTGAACT CCCCTGCCAG GATGGCACAT ATGGGCTGAA CTGTGCGGAG
    CGCTGTGACT GTAGCCATGC GGATGGCTGC CACTCCACCA CGGGCCACTG CCGCTGCCTC
    CCTGGATGGT CAGGTGTCCA CTGCGACAGC GTGTGTGCTG AGGGGCGCTG GGGGCCCAAC
    TGCTCTCTGC CCTGCTACTG TAAAAATGGG GCTTCATGCT CCCCAGACGA TGGCATCTGT
    GAGTGTGCGC CAGGGTTCCG AGGCACCACC TGTCAGAGAA TCTGCTCCCC TGGTTTTTAT
    GGGCATCGCT GCAGCCAGAC ATGCCCGCAG TGCGTGCATA GCAGTGGGCC CTGCCACCAC
    ATCACAGGCC TGTGTGACTG CTTGCCTGGC TTCACAGGTG CCCTCTGCAA TGAAGTGTGT
    CCCAGTGGCA GATTTGGGAA AAACTGTGCT GGAGTTTGTA CCTGTACAAA CAATGGAACC
    TGTAACCCCA TTGACCGATC TTGTCAGTGT TATCCAGGGT GGATTGGCAG TGACTGCTCG
    CAACCTTGTC CTCCTGCGCA TTGGGGTCCA AACTGCATCC ACACGTGCAA CTGCCACAAT
    GGGGCGTTCT GCAGTGCATA CGACGGGGAA TGTAAATGCA CTCCTGGCTG GACCGGACTC
    TACTGCACTC AGAGATGTCC CCTGGGTTTT TACGGAAAGG ACTGTGCGTT GATATGTCAG
    TGTCAGAATG GAGCTGACTG TGACCACATC TCTGGGCAGT GTACATGCCG CACTGGGTTC
    ATGGGGAAGC ACTGTGAACA GAAATGCCCT GCGGGAACAT ATGGCTATGG CTGTCGCCAG
    ATATGTGACT GTCTGAACAA CTCCACCTGT GACCATATCA CTGGGACTTG TTACTGTAGC
    CCAGGCTGGA AGGGGGCACG GTGTGATCAA GCTGGAGTCA TTATCGTTGG AAATCTGAAC
    AGCTTAAGCA GGACCAGTAC AGCTCTCCCT GCCAATTCCT ACCAGATCGG AGCCATCGTG
    GGCATCATCA TTCTTGTACT GGTTGTTCTC TTCCTCCTGG CGTTGTTCAT TATTTACAGA
    CAGAAGCAAA AGGGAAAGGA ATCAAGCATG CCTGCAGTTA CCTACACCCC TGCCCTGAGG
    GTCATGAATG CAGACTATAC CATTTCAGAA ACCCTTCCCC ACAGCAACAG TGGAAGTGCT
    AACAGCCATT ACTTCACTAA CCCCAGTTAC CACACACTTA CCCAGTGCGC CACATCCCCA
    CATGTCAACA ACAGGGACAG GATGACCATC GCAAAGTCAA AGAACAATCA GCTGTTTGTG
    AATCTGAAAA ATGTGAATCC TGGGAAGAGA GGCCCAGTGG CAGACTGCAC GGGAACATTG
    CCAGCAGACT GGAAACATGG CGGCTACCTT AATGAGCTTG GAGCTTTTGG ACTTGATAGA
    AGTTATATGG GAAAATCCTT GAAAGATTTG GGAAAGAATT CTGAATATAA TTCAAGTAAC
    TGCTCCTTAA GCAGTTCTGA AAACCCATAT GCCACTATTA AAGACCCGCC TGTACTTATC
    CCCAAAAACT CAGAGTGTGG CTATGTGGAA ATGAAGTCAC CAGCACGGAG AGATTCCCCC
    TATGCAGAGA TCAATAACTC AACTTCAGCC AACAAGAATG TCTATGAAGT TGAACCTACA
    GTGAGTGTTG TCCAAGGAAT ATTCAGCAAT AATGGGCATC TCACCCAGGA TCCATACGAC
    CTCCCAAAGA ACAGTCACAT CCCTTGCCAT TATGACCTGC TGCCAGTCCG AGACAGTTCA
    TCCTCCCCCA AGCGAGAGGA TGGTGGCAGT GGCAGCAGCA GCAGCAGTGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe14211
    Clone ID Related Accession (Same CDS sequence) XM_014117692.1 , XM_014117690.2 , XM_005626476.2 , XM_531893.6 , XM_014117690.1 , XM_014117691.1 , XM_005626476.3 , XM_531893.5 , XM_014117691.2
    Accession Version XM_014117691.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3414bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product multiple epidermal growth factor-like domains protein 10 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGTTGCTT CTTTGAACTC ATGCCTGAGC TTTATCTGTT TCTCATTATG CCTCTGGAGC 
    AGGACAGCAT CAGCACTGAA TCTTGAAGAC CCTAATGTGT GTAGCCACTG GGAAAGCTAC
    TCAGTGACTG TGCAAGAGTC ATACCCACAT CCTTTCGATC AAATCTACTA CACCAGCTGC
    ACTGATATTC TGAACTGGTT TAAATGTACA CGGCACAGAA TCAGTTATCG GACTGCCTAT
    CGACATGGGG AGAAAACTAT GTATAGGCGC AAATCCCAGT GTTGTCCTGG ATTTTATGAA
    AGCAGGGAAA TGTGTGTCCC CCACTGTGCT GATAAATGTG TCCATGGTCG CTGCATTGCT
    CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGTGGAACCA ACTGCTCCAG TGCCTGTGAT
    GGTGATCACT GGGGGCCCCA TTGCAGCAGC CGGTGCCAGT GCAAGAACGG GGCCCTGTGC
    AACCCCATCA CGGGGGCTTG CCACTGCGCT GCAGGTTTCC GGGGCTGGCG TTGTGAGGAC
    CGCTGTGAGC AGGGCACCTA CGGTAATGAC TGTCACCAGA GATGCCAGTG TCAGAATGGA
    GCCACCTGCG ACCACGTCAC AGGGGAATGC CGCTGCCCAC CAGGGTACAC TGGAGCCTTC
    TGTGAGGACC TTTGTCCCCC TGGCAAGCAC GGTCCGCAGT GCGAGCAGAG ATGTCCCTGC
    CAAAATGGAG GAATATGTCA TCACGTCACC GGAGAATGCT CTTGCCCTTC GGGCTGGCTG
    GGCACAGTCT GTGGTCAGCC TTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCACAAGAA
    TGTCAGTGCC ATAATGGAGG GACGTGTGAT GCTGCCACAG GCCAATGTCA TTGCAGTCCA
    GGATACACAG GGGAACGCTG CCAGGACGAG TGTCCCGTTG GCACATACGG AGTTCGCTGT
    GCTGAGACCT GCCAGTGTGT CAATGGAGGG AAGTGTTATC ACGTGAGTGG CTCATGCCTC
    TGTGAAGCAG GCTTTGCTGG TGAGCACTGT GAGGCACGCC TGTGTCCCGA AGGGCTCTAC
    GGCATCAAAT GTGACAAGCG GTGTCCCTGC CACTTGGACA ACACTCATAG CTGTCACCCC
    ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC TGGTCAGGAC TCTACTGTAA CGAAACATGT
    TCTCCTGGAT TCTTTGGGGA AGCTTGCCAA CAGATCTGCA GCTGCCACAA CGGGGCTGAT
    TGTGACAGTG TGACTGGAAA ATGCATCTGT GCCCCAGGAT TCAAAGGAAT TGACTGCTCT
    ATCCCATGCC CATTGGAAAC CTATGGGATA AACTGCTCCT CTCTCTGTGG CTGTAAAAAC
    GATGCTGTCT GTTCACCTGT AGATGGGTCT TGTACTTGCA AGGCAGGCTG GCACGGGGTG
    GACTGCTCCA TCAGCTGTCC CAGTGGCACG TGGGGCTTCA GCTGTAACCT GACCTGCCAG
    TGCCTCCACG GGGGAGCCTG CAACACCCAG GACGGGACCT GCACGTGTGC GCCTGGATGG
    CGCGGGGACA AATGTGAACT CCCCTGCCAG GATGGCACAT ATGGGCTGAA CTGTGCGGAG
    CGCTGTGACT GTAGCCATGC GGATGGCTGC CACTCCACCA CGGGCCACTG CCGCTGCCTC
    CCTGGATGGT CAGGTGTCCA CTGCGACAGC GTGTGTGCTG AGGGGCGCTG GGGGCCCAAC
    TGCTCTCTGC CCTGCTACTG TAAAAATGGG GCTTCATGCT CCCCAGACGA TGGCATCTGT
    GAGTGTGCGC CAGGGTTCCG AGGCACCACC TGTCAGAGAA TCTGCTCCCC TGGTTTTTAT
    GGGCATCGCT GCAGCCAGAC ATGCCCGCAG TGCGTGCATA GCAGTGGGCC CTGCCACCAC
    ATCACAGGCC TGTGTGACTG CTTGCCTGGC TTCACAGGTG CCCTCTGCAA TGAAGTGTGT
    CCCAGTGGCA GATTTGGGAA AAACTGTGCT GGAGTTTGTA CCTGTACAAA CAATGGAACC
    TGTAACCCCA TTGACCGATC TTGTCAGTGT TATCCAGGGT GGATTGGCAG TGACTGCTCG
    CAACCTTGTC CTCCTGCGCA TTGGGGTCCA AACTGCATCC ACACGTGCAA CTGCCACAAT
    GGGGCGTTCT GCAGTGCATA CGACGGGGAA TGTAAATGCA CTCCTGGCTG GACCGGACTC
    TACTGCACTC AGAGATGTCC CCTGGGTTTT TACGGAAAGG ACTGTGCGTT GATATGTCAG
    TGTCAGAATG GAGCTGACTG TGACCACATC TCTGGGCAGT GTACATGCCG CACTGGGTTC
    ATGGGGAAGC ACTGTGAACA GAAATGCCCT GCGGGAACAT ATGGCTATGG CTGTCGCCAG
    ATATGTGACT GTCTGAACAA CTCCACCTGT GACCATATCA CTGGGACTTG TTACTGTAGC
    CCAGGCTGGA AGGGGGCACG GTGTGATCAA GCTGGAGTCA TTATCGTTGG AAATCTGAAC
    AGCTTAAGCA GGACCAGTAC AGCTCTCCCT GCCAATTCCT ACCAGATCGG AGCCATCGTG
    GGCATCATCA TTCTTGTACT GGTTGTTCTC TTCCTCCTGG CGTTGTTCAT TATTTACAGA
    CAGAAGCAAA AGGGAAAGGA ATCAAGCATG CCTGCAGTTA CCTACACCCC TGCCCTGAGG
    GTCATGAATG CAGACTATAC CATTTCAGAA ACCCTTCCCC ACAGCAACAG TGGAAGTGCT
    AACAGCCATT ACTTCACTAA CCCCAGTTAC CACACACTTA CCCAGTGCGC CACATCCCCA
    CATGTCAACA ACAGGGACAG GATGACCATC GCAAAGTCAA AGAACAATCA GCTGTTTGTG
    AATCTGAAAA ATGTGAATCC TGGGAAGAGA GGCCCAGTGG CAGACTGCAC GGGAACATTG
    CCAGCAGACT GGAAACATGG CGGCTACCTT AATGAGCTTG GAGCTTTTGG ACTTGATAGA
    AGTTATATGG GAAAATCCTT GAAAGATTTG GGAAAGAATT CTGAATATAA TTCAAGTAAC
    TGCTCCTTAA GCAGTTCTGA AAACCCATAT GCCACTATTA AAGACCCGCC TGTACTTATC
    CCCAAAAACT CAGAGTGTGG CTATGTGGAA ATGAAGTCAC CAGCACGGAG AGATTCCCCC
    TATGCAGAGA TCAATAACTC AACTTCAGCC AACAAGAATG TCTATGAAGT TGAACCTACA
    GTGAGTGTTG TCCAAGGAAT ATTCAGCAAT AATGGGCATC TCACCCAGGA TCCATACGAC
    CTCCCAAAGA ACAGTCACAT CCCTTGCCAT TATGACCTGC TGCCAGTCCG AGACAGTTCA
    TCCTCCCCCA AGCGAGAGGA TGGTGGCAGT GGCAGCAGCA GCAGCAGTGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973166.1
    CDS166..3579
    Translation

    Target ORF information:

    RefSeq Version XM_014117691.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris multiple EGF-like-domains 10 (MEGF10), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014117691.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGTTGCTT CTTTGAACTC ATGCCTGAGC TTTATCTGTT TCTCATTATG CCTCTGGAGC 
    AGGACAGCAT CAGCACTGAA TCTTGAAGAC CCTAATGTGT GTAGCCACTG GGAAAGCTAC
    TCAGTGACTG TGCAAGAGTC ATACCCACAT CCTTTCGATC AAATCTACTA CACCAGCTGC
    ACTGATATTC TGAACTGGTT TAAATGTACA CGGCACAGAA TCAGTTATCG GACTGCCTAT
    CGACATGGGG AGAAAACTAT GTATAGGCGC AAATCCCAGT GTTGTCCTGG ATTTTATGAA
    AGCAGGGAAA TGTGTGTCCC CCACTGTGCT GATAAATGTG TCCATGGTCG CTGCATTGCT
    CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGTGGAACCA ACTGCTCCAG TGCCTGTGAT
    GGTGATCACT GGGGGCCCCA TTGCAGCAGC CGGTGCCAGT GCAAGAACGG GGCCCTGTGC
    AACCCCATCA CGGGGGCTTG CCACTGCGCT GCAGGTTTCC GGGGCTGGCG TTGTGAGGAC
    CGCTGTGAGC AGGGCACCTA CGGTAATGAC TGTCACCAGA GATGCCAGTG TCAGAATGGA
    GCCACCTGCG ACCACGTCAC AGGGGAATGC CGCTGCCCAC CAGGGTACAC TGGAGCCTTC
    TGTGAGGACC TTTGTCCCCC TGGCAAGCAC GGTCCGCAGT GCGAGCAGAG ATGTCCCTGC
    CAAAATGGAG GAATATGTCA TCACGTCACC GGAGAATGCT CTTGCCCTTC GGGCTGGCTG
    GGCACAGTCT GTGGTCAGCC TTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCACAAGAA
    TGTCAGTGCC ATAATGGAGG GACGTGTGAT GCTGCCACAG GCCAATGTCA TTGCAGTCCA
    GGATACACAG GGGAACGCTG CCAGGACGAG TGTCCCGTTG GCACATACGG AGTTCGCTGT
    GCTGAGACCT GCCAGTGTGT CAATGGAGGG AAGTGTTATC ACGTGAGTGG CTCATGCCTC
    TGTGAAGCAG GCTTTGCTGG TGAGCACTGT GAGGCACGCC TGTGTCCCGA AGGGCTCTAC
    GGCATCAAAT GTGACAAGCG GTGTCCCTGC CACTTGGACA ACACTCATAG CTGTCACCCC
    ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC TGGTCAGGAC TCTACTGTAA CGAAACATGT
    TCTCCTGGAT TCTTTGGGGA AGCTTGCCAA CAGATCTGCA GCTGCCACAA CGGGGCTGAT
    TGTGACAGTG TGACTGGAAA ATGCATCTGT GCCCCAGGAT TCAAAGGAAT TGACTGCTCT
    ATCCCATGCC CATTGGAAAC CTATGGGATA AACTGCTCCT CTCTCTGTGG CTGTAAAAAC
    GATGCTGTCT GTTCACCTGT AGATGGGTCT TGTACTTGCA AGGCAGGCTG GCACGGGGTG
    GACTGCTCCA TCAGCTGTCC CAGTGGCACG TGGGGCTTCA GCTGTAACCT GACCTGCCAG
    TGCCTCCACG GGGGAGCCTG CAACACCCAG GACGGGACCT GCACGTGTGC GCCTGGATGG
    CGCGGGGACA AATGTGAACT CCCCTGCCAG GATGGCACAT ATGGGCTGAA CTGTGCGGAG
    CGCTGTGACT GTAGCCATGC GGATGGCTGC CACTCCACCA CGGGCCACTG CCGCTGCCTC
    CCTGGATGGT CAGGTGTCCA CTGCGACAGC GTGTGTGCTG AGGGGCGCTG GGGGCCCAAC
    TGCTCTCTGC CCTGCTACTG TAAAAATGGG GCTTCATGCT CCCCAGACGA TGGCATCTGT
    GAGTGTGCGC CAGGGTTCCG AGGCACCACC TGTCAGAGAA TCTGCTCCCC TGGTTTTTAT
    GGGCATCGCT GCAGCCAGAC ATGCCCGCAG TGCGTGCATA GCAGTGGGCC CTGCCACCAC
    ATCACAGGCC TGTGTGACTG CTTGCCTGGC TTCACAGGTG CCCTCTGCAA TGAAGTGTGT
    CCCAGTGGCA GATTTGGGAA AAACTGTGCT GGAGTTTGTA CCTGTACAAA CAATGGAACC
    TGTAACCCCA TTGACCGATC TTGTCAGTGT TATCCAGGGT GGATTGGCAG TGACTGCTCG
    CAACCTTGTC CTCCTGCGCA TTGGGGTCCA AACTGCATCC ACACGTGCAA CTGCCACAAT
    GGGGCGTTCT GCAGTGCATA CGACGGGGAA TGTAAATGCA CTCCTGGCTG GACCGGACTC
    TACTGCACTC AGAGATGTCC CCTGGGTTTT TACGGAAAGG ACTGTGCGTT GATATGTCAG
    TGTCAGAATG GAGCTGACTG TGACCACATC TCTGGGCAGT GTACATGCCG CACTGGGTTC
    ATGGGGAAGC ACTGTGAACA GAAATGCCCT GCGGGAACAT ATGGCTATGG CTGTCGCCAG
    ATATGTGACT GTCTGAACAA CTCCACCTGT GACCATATCA CTGGGACTTG TTACTGTAGC
    CCAGGCTGGA AGGGGGCACG GTGTGATCAA GCTGGAGTCA TTATCGTTGG AAATCTGAAC
    AGCTTAAGCA GGACCAGTAC AGCTCTCCCT GCCAATTCCT ACCAGATCGG AGCCATCGTG
    GGCATCATCA TTCTTGTACT GGTTGTTCTC TTCCTCCTGG CGTTGTTCAT TATTTACAGA
    CAGAAGCAAA AGGGAAAGGA ATCAAGCATG CCTGCAGTTA CCTACACCCC TGCCCTGAGG
    GTCATGAATG CAGACTATAC CATTTCAGAA ACCCTTCCCC ACAGCAACAG TGGAAGTGCT
    AACAGCCATT ACTTCACTAA CCCCAGTTAC CACACACTTA CCCAGTGCGC CACATCCCCA
    CATGTCAACA ACAGGGACAG GATGACCATC GCAAAGTCAA AGAACAATCA GCTGTTTGTG
    AATCTGAAAA ATGTGAATCC TGGGAAGAGA GGCCCAGTGG CAGACTGCAC GGGAACATTG
    CCAGCAGACT GGAAACATGG CGGCTACCTT AATGAGCTTG GAGCTTTTGG ACTTGATAGA
    AGTTATATGG GAAAATCCTT GAAAGATTTG GGAAAGAATT CTGAATATAA TTCAAGTAAC
    TGCTCCTTAA GCAGTTCTGA AAACCCATAT GCCACTATTA AAGACCCGCC TGTACTTATC
    CCCAAAAACT CAGAGTGTGG CTATGTGGAA ATGAAGTCAC CAGCACGGAG AGATTCCCCC
    TATGCAGAGA TCAATAACTC AACTTCAGCC AACAAGAATG TCTATGAAGT TGAACCTACA
    GTGAGTGTTG TCCAAGGAAT ATTCAGCAAT AATGGGCATC TCACCCAGGA TCCATACGAC
    CTCCCAAAGA ACAGTCACAT CCCTTGCCAT TATGACCTGC TGCCAGTCCG AGACAGTTCA
    TCCTCCCCCA AGCGAGAGGA TGGTGGCAGT GGCAGCAGCA GCAGCAGTGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe14211
    Clone ID Related Accession (Same CDS sequence) XM_014117692.1 , XM_014117690.2 , XM_005626476.2 , XM_531893.6 , XM_014117690.1 , XM_014117691.1 , XM_005626476.3 , XM_531893.5 , XM_014117691.2
    Accession Version XM_005626476.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3414bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product multiple epidermal growth factor-like domains protein 10 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005626476.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGTTGCTT CTTTGAACTC ATGCCTGAGC TTTATCTGTT TCTCATTATG CCTCTGGAGC 
    AGGACAGCAT CAGCACTGAA TCTTGAAGAC CCTAATGTGT GTAGCCACTG GGAAAGCTAC
    TCAGTGACTG TGCAAGAGTC ATACCCACAT CCTTTCGATC AAATCTACTA CACCAGCTGC
    ACTGATATTC TGAACTGGTT TAAATGTACA CGGCACAGAA TCAGTTATCG GACTGCCTAT
    CGACATGGGG AGAAAACTAT GTATAGGCGC AAATCCCAGT GTTGTCCTGG ATTTTATGAA
    AGCAGGGAAA TGTGTGTCCC CCACTGTGCT GATAAATGTG TCCATGGTCG CTGCATTGCT
    CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGTGGAACCA ACTGCTCCAG TGCCTGTGAT
    GGTGATCACT GGGGGCCCCA TTGCAGCAGC CGGTGCCAGT GCAAGAACGG GGCCCTGTGC
    AACCCCATCA CGGGGGCTTG CCACTGCGCT GCAGGTTTCC GGGGCTGGCG TTGTGAGGAC
    CGCTGTGAGC AGGGCACCTA CGGTAATGAC TGTCACCAGA GATGCCAGTG TCAGAATGGA
    GCCACCTGCG ACCACGTCAC AGGGGAATGC CGCTGCCCAC CAGGGTACAC TGGAGCCTTC
    TGTGAGGACC TTTGTCCCCC TGGCAAGCAC GGTCCGCAGT GCGAGCAGAG ATGTCCCTGC
    CAAAATGGAG GAATATGTCA TCACGTCACC GGAGAATGCT CTTGCCCTTC GGGCTGGCTG
    GGCACAGTCT GTGGTCAGCC TTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCACAAGAA
    TGTCAGTGCC ATAATGGAGG GACGTGTGAT GCTGCCACAG GCCAATGTCA TTGCAGTCCA
    GGATACACAG GGGAACGCTG CCAGGACGAG TGTCCCGTTG GCACATACGG AGTTCGCTGT
    GCTGAGACCT GCCAGTGTGT CAATGGAGGG AAGTGTTATC ACGTGAGTGG CTCATGCCTC
    TGTGAAGCAG GCTTTGCTGG TGAGCACTGT GAGGCACGCC TGTGTCCCGA AGGGCTCTAC
    GGCATCAAAT GTGACAAGCG GTGTCCCTGC CACTTGGACA ACACTCATAG CTGTCACCCC
    ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC TGGTCAGGAC TCTACTGTAA CGAAACATGT
    TCTCCTGGAT TCTTTGGGGA AGCTTGCCAA CAGATCTGCA GCTGCCACAA CGGGGCTGAT
    TGTGACAGTG TGACTGGAAA ATGCATCTGT GCCCCAGGAT TCAAAGGAAT TGACTGCTCT
    ATCCCATGCC CATTGGAAAC CTATGGGATA AACTGCTCCT CTCTCTGTGG CTGTAAAAAC
    GATGCTGTCT GTTCACCTGT AGATGGGTCT TGTACTTGCA AGGCAGGCTG GCACGGGGTG
    GACTGCTCCA TCAGCTGTCC CAGTGGCACG TGGGGCTTCA GCTGTAACCT GACCTGCCAG
    TGCCTCCACG GGGGAGCCTG CAACACCCAG GACGGGACCT GCACGTGTGC GCCTGGATGG
    CGCGGGGACA AATGTGAACT CCCCTGCCAG GATGGCACAT ATGGGCTGAA CTGTGCGGAG
    CGCTGTGACT GTAGCCATGC GGATGGCTGC CACTCCACCA CGGGCCACTG CCGCTGCCTC
    CCTGGATGGT CAGGTGTCCA CTGCGACAGC GTGTGTGCTG AGGGGCGCTG GGGGCCCAAC
    TGCTCTCTGC CCTGCTACTG TAAAAATGGG GCTTCATGCT CCCCAGACGA TGGCATCTGT
    GAGTGTGCGC CAGGGTTCCG AGGCACCACC TGTCAGAGAA TCTGCTCCCC TGGTTTTTAT
    GGGCATCGCT GCAGCCAGAC ATGCCCGCAG TGCGTGCATA GCAGTGGGCC CTGCCACCAC
    ATCACAGGCC TGTGTGACTG CTTGCCTGGC TTCACAGGTG CCCTCTGCAA TGAAGTGTGT
    CCCAGTGGCA GATTTGGGAA AAACTGTGCT GGAGTTTGTA CCTGTACAAA CAATGGAACC
    TGTAACCCCA TTGACCGATC TTGTCAGTGT TATCCAGGGT GGATTGGCAG TGACTGCTCG
    CAACCTTGTC CTCCTGCGCA TTGGGGTCCA AACTGCATCC ACACGTGCAA CTGCCACAAT
    GGGGCGTTCT GCAGTGCATA CGACGGGGAA TGTAAATGCA CTCCTGGCTG GACCGGACTC
    TACTGCACTC AGAGATGTCC CCTGGGTTTT TACGGAAAGG ACTGTGCGTT GATATGTCAG
    TGTCAGAATG GAGCTGACTG TGACCACATC TCTGGGCAGT GTACATGCCG CACTGGGTTC
    ATGGGGAAGC ACTGTGAACA GAAATGCCCT GCGGGAACAT ATGGCTATGG CTGTCGCCAG
    ATATGTGACT GTCTGAACAA CTCCACCTGT GACCATATCA CTGGGACTTG TTACTGTAGC
    CCAGGCTGGA AGGGGGCACG GTGTGATCAA GCTGGAGTCA TTATCGTTGG AAATCTGAAC
    AGCTTAAGCA GGACCAGTAC AGCTCTCCCT GCCAATTCCT ACCAGATCGG AGCCATCGTG
    GGCATCATCA TTCTTGTACT GGTTGTTCTC TTCCTCCTGG CGTTGTTCAT TATTTACAGA
    CAGAAGCAAA AGGGAAAGGA ATCAAGCATG CCTGCAGTTA CCTACACCCC TGCCCTGAGG
    GTCATGAATG CAGACTATAC CATTTCAGAA ACCCTTCCCC ACAGCAACAG TGGAAGTGCT
    AACAGCCATT ACTTCACTAA CCCCAGTTAC CACACACTTA CCCAGTGCGC CACATCCCCA
    CATGTCAACA ACAGGGACAG GATGACCATC GCAAAGTCAA AGAACAATCA GCTGTTTGTG
    AATCTGAAAA ATGTGAATCC TGGGAAGAGA GGCCCAGTGG CAGACTGCAC GGGAACATTG
    CCAGCAGACT GGAAACATGG CGGCTACCTT AATGAGCTTG GAGCTTTTGG ACTTGATAGA
    AGTTATATGG GAAAATCCTT GAAAGATTTG GGAAAGAATT CTGAATATAA TTCAAGTAAC
    TGCTCCTTAA GCAGTTCTGA AAACCCATAT GCCACTATTA AAGACCCGCC TGTACTTATC
    CCCAAAAACT CAGAGTGTGG CTATGTGGAA ATGAAGTCAC CAGCACGGAG AGATTCCCCC
    TATGCAGAGA TCAATAACTC AACTTCAGCC AACAAGAATG TCTATGAAGT TGAACCTACA
    GTGAGTGTTG TCCAAGGAAT ATTCAGCAAT AATGGGCATC TCACCCAGGA TCCATACGAC
    CTCCCAAAGA ACAGTCACAT CCCTTGCCAT TATGACCTGC TGCCAGTCCG AGACAGTTCA
    TCCTCCCCCA AGCGAGAGGA TGGTGGCAGT GGCAGCAGCA GCAGCAGTGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005626533.1
    CDS818..4231
    Translation

    Target ORF information:

    RefSeq Version XM_005626476.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris multiple EGF like domains 10 (MEGF10), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005626476.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGTTGCTT CTTTGAACTC ATGCCTGAGC TTTATCTGTT TCTCATTATG CCTCTGGAGC 
    AGGACAGCAT CAGCACTGAA TCTTGAAGAC CCTAATGTGT GTAGCCACTG GGAAAGCTAC
    TCAGTGACTG TGCAAGAGTC ATACCCACAT CCTTTCGATC AAATCTACTA CACCAGCTGC
    ACTGATATTC TGAACTGGTT TAAATGTACA CGGCACAGAA TCAGTTATCG GACTGCCTAT
    CGACATGGGG AGAAAACTAT GTATAGGCGC AAATCCCAGT GTTGTCCTGG ATTTTATGAA
    AGCAGGGAAA TGTGTGTCCC CCACTGTGCT GATAAATGTG TCCATGGTCG CTGCATTGCT
    CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGTGGAACCA ACTGCTCCAG TGCCTGTGAT
    GGTGATCACT GGGGGCCCCA TTGCAGCAGC CGGTGCCAGT GCAAGAACGG GGCCCTGTGC
    AACCCCATCA CGGGGGCTTG CCACTGCGCT GCAGGTTTCC GGGGCTGGCG TTGTGAGGAC
    CGCTGTGAGC AGGGCACCTA CGGTAATGAC TGTCACCAGA GATGCCAGTG TCAGAATGGA
    GCCACCTGCG ACCACGTCAC AGGGGAATGC CGCTGCCCAC CAGGGTACAC TGGAGCCTTC
    TGTGAGGACC TTTGTCCCCC TGGCAAGCAC GGTCCGCAGT GCGAGCAGAG ATGTCCCTGC
    CAAAATGGAG GAATATGTCA TCACGTCACC GGAGAATGCT CTTGCCCTTC GGGCTGGCTG
    GGCACAGTCT GTGGTCAGCC TTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCACAAGAA
    TGTCAGTGCC ATAATGGAGG GACGTGTGAT GCTGCCACAG GCCAATGTCA TTGCAGTCCA
    GGATACACAG GGGAACGCTG CCAGGACGAG TGTCCCGTTG GCACATACGG AGTTCGCTGT
    GCTGAGACCT GCCAGTGTGT CAATGGAGGG AAGTGTTATC ACGTGAGTGG CTCATGCCTC
    TGTGAAGCAG GCTTTGCTGG TGAGCACTGT GAGGCACGCC TGTGTCCCGA AGGGCTCTAC
    GGCATCAAAT GTGACAAGCG GTGTCCCTGC CACTTGGACA ACACTCATAG CTGTCACCCC
    ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC TGGTCAGGAC TCTACTGTAA CGAAACATGT
    TCTCCTGGAT TCTTTGGGGA AGCTTGCCAA CAGATCTGCA GCTGCCACAA CGGGGCTGAT
    TGTGACAGTG TGACTGGAAA ATGCATCTGT GCCCCAGGAT TCAAAGGAAT TGACTGCTCT
    ATCCCATGCC CATTGGAAAC CTATGGGATA AACTGCTCCT CTCTCTGTGG CTGTAAAAAC
    GATGCTGTCT GTTCACCTGT AGATGGGTCT TGTACTTGCA AGGCAGGCTG GCACGGGGTG
    GACTGCTCCA TCAGCTGTCC CAGTGGCACG TGGGGCTTCA GCTGTAACCT GACCTGCCAG
    TGCCTCCACG GGGGAGCCTG CAACACCCAG GACGGGACCT GCACGTGTGC GCCTGGATGG
    CGCGGGGACA AATGTGAACT CCCCTGCCAG GATGGCACAT ATGGGCTGAA CTGTGCGGAG
    CGCTGTGACT GTAGCCATGC GGATGGCTGC CACTCCACCA CGGGCCACTG CCGCTGCCTC
    CCTGGATGGT CAGGTGTCCA CTGCGACAGC GTGTGTGCTG AGGGGCGCTG GGGGCCCAAC
    TGCTCTCTGC CCTGCTACTG TAAAAATGGG GCTTCATGCT CCCCAGACGA TGGCATCTGT
    GAGTGTGCGC CAGGGTTCCG AGGCACCACC TGTCAGAGAA TCTGCTCCCC TGGTTTTTAT
    GGGCATCGCT GCAGCCAGAC ATGCCCGCAG TGCGTGCATA GCAGTGGGCC CTGCCACCAC
    ATCACAGGCC TGTGTGACTG CTTGCCTGGC TTCACAGGTG CCCTCTGCAA TGAAGTGTGT
    CCCAGTGGCA GATTTGGGAA AAACTGTGCT GGAGTTTGTA CCTGTACAAA CAATGGAACC
    TGTAACCCCA TTGACCGATC TTGTCAGTGT TATCCAGGGT GGATTGGCAG TGACTGCTCG
    CAACCTTGTC CTCCTGCGCA TTGGGGTCCA AACTGCATCC ACACGTGCAA CTGCCACAAT
    GGGGCGTTCT GCAGTGCATA CGACGGGGAA TGTAAATGCA CTCCTGGCTG GACCGGACTC
    TACTGCACTC AGAGATGTCC CCTGGGTTTT TACGGAAAGG ACTGTGCGTT GATATGTCAG
    TGTCAGAATG GAGCTGACTG TGACCACATC TCTGGGCAGT GTACATGCCG CACTGGGTTC
    ATGGGGAAGC ACTGTGAACA GAAATGCCCT GCGGGAACAT ATGGCTATGG CTGTCGCCAG
    ATATGTGACT GTCTGAACAA CTCCACCTGT GACCATATCA CTGGGACTTG TTACTGTAGC
    CCAGGCTGGA AGGGGGCACG GTGTGATCAA GCTGGAGTCA TTATCGTTGG AAATCTGAAC
    AGCTTAAGCA GGACCAGTAC AGCTCTCCCT GCCAATTCCT ACCAGATCGG AGCCATCGTG
    GGCATCATCA TTCTTGTACT GGTTGTTCTC TTCCTCCTGG CGTTGTTCAT TATTTACAGA
    CAGAAGCAAA AGGGAAAGGA ATCAAGCATG CCTGCAGTTA CCTACACCCC TGCCCTGAGG
    GTCATGAATG CAGACTATAC CATTTCAGAA ACCCTTCCCC ACAGCAACAG TGGAAGTGCT
    AACAGCCATT ACTTCACTAA CCCCAGTTAC CACACACTTA CCCAGTGCGC CACATCCCCA
    CATGTCAACA ACAGGGACAG GATGACCATC GCAAAGTCAA AGAACAATCA GCTGTTTGTG
    AATCTGAAAA ATGTGAATCC TGGGAAGAGA GGCCCAGTGG CAGACTGCAC GGGAACATTG
    CCAGCAGACT GGAAACATGG CGGCTACCTT AATGAGCTTG GAGCTTTTGG ACTTGATAGA
    AGTTATATGG GAAAATCCTT GAAAGATTTG GGAAAGAATT CTGAATATAA TTCAAGTAAC
    TGCTCCTTAA GCAGTTCTGA AAACCCATAT GCCACTATTA AAGACCCGCC TGTACTTATC
    CCCAAAAACT CAGAGTGTGG CTATGTGGAA ATGAAGTCAC CAGCACGGAG AGATTCCCCC
    TATGCAGAGA TCAATAACTC AACTTCAGCC AACAAGAATG TCTATGAAGT TGAACCTACA
    GTGAGTGTTG TCCAAGGAAT ATTCAGCAAT AATGGGCATC TCACCCAGGA TCCATACGAC
    CTCCCAAAGA ACAGTCACAT CCCTTGCCAT TATGACCTGC TGCCAGTCCG AGACAGTTCA
    TCCTCCCCCA AGCGAGAGGA TGGTGGCAGT GGCAGCAGCA GCAGCAGTGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe14211
    Clone ID Related Accession (Same CDS sequence) XM_014117692.1 , XM_014117690.2 , XM_005626476.2 , XM_531893.6 , XM_014117690.1 , XM_014117691.1 , XM_005626476.3 , XM_531893.5 , XM_014117691.2
    Accession Version XM_531893.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3414bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product multiple epidermal growth factor-like domains protein 10 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_531893.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGTTGCTT CTTTGAACTC ATGCCTGAGC TTTATCTGTT TCTCATTATG CCTCTGGAGC 
    AGGACAGCAT CAGCACTGAA TCTTGAAGAC CCTAATGTGT GTAGCCACTG GGAAAGCTAC
    TCAGTGACTG TGCAAGAGTC ATACCCACAT CCTTTCGATC AAATCTACTA CACCAGCTGC
    ACTGATATTC TGAACTGGTT TAAATGTACA CGGCACAGAA TCAGTTATCG GACTGCCTAT
    CGACATGGGG AGAAAACTAT GTATAGGCGC AAATCCCAGT GTTGTCCTGG ATTTTATGAA
    AGCAGGGAAA TGTGTGTCCC CCACTGTGCT GATAAATGTG TCCATGGTCG CTGCATTGCT
    CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGTGGAACCA ACTGCTCCAG TGCCTGTGAT
    GGTGATCACT GGGGGCCCCA TTGCAGCAGC CGGTGCCAGT GCAAGAACGG GGCCCTGTGC
    AACCCCATCA CGGGGGCTTG CCACTGCGCT GCAGGTTTCC GGGGCTGGCG TTGTGAGGAC
    CGCTGTGAGC AGGGCACCTA CGGTAATGAC TGTCACCAGA GATGCCAGTG TCAGAATGGA
    GCCACCTGCG ACCACGTCAC AGGGGAATGC CGCTGCCCAC CAGGGTACAC TGGAGCCTTC
    TGTGAGGACC TTTGTCCCCC TGGCAAGCAC GGTCCGCAGT GCGAGCAGAG ATGTCCCTGC
    CAAAATGGAG GAATATGTCA TCACGTCACC GGAGAATGCT CTTGCCCTTC GGGCTGGCTG
    GGCACAGTCT GTGGTCAGCC TTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCACAAGAA
    TGTCAGTGCC ATAATGGAGG GACGTGTGAT GCTGCCACAG GCCAATGTCA TTGCAGTCCA
    GGATACACAG GGGAACGCTG CCAGGACGAG TGTCCCGTTG GCACATACGG AGTTCGCTGT
    GCTGAGACCT GCCAGTGTGT CAATGGAGGG AAGTGTTATC ACGTGAGTGG CTCATGCCTC
    TGTGAAGCAG GCTTTGCTGG TGAGCACTGT GAGGCACGCC TGTGTCCCGA AGGGCTCTAC
    GGCATCAAAT GTGACAAGCG GTGTCCCTGC CACTTGGACA ACACTCATAG CTGTCACCCC
    ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC TGGTCAGGAC TCTACTGTAA CGAAACATGT
    TCTCCTGGAT TCTTTGGGGA AGCTTGCCAA CAGATCTGCA GCTGCCACAA CGGGGCTGAT
    TGTGACAGTG TGACTGGAAA ATGCATCTGT GCCCCAGGAT TCAAAGGAAT TGACTGCTCT
    ATCCCATGCC CATTGGAAAC CTATGGGATA AACTGCTCCT CTCTCTGTGG CTGTAAAAAC
    GATGCTGTCT GTTCACCTGT AGATGGGTCT TGTACTTGCA AGGCAGGCTG GCACGGGGTG
    GACTGCTCCA TCAGCTGTCC CAGTGGCACG TGGGGCTTCA GCTGTAACCT GACCTGCCAG
    TGCCTCCACG GGGGAGCCTG CAACACCCAG GACGGGACCT GCACGTGTGC GCCTGGATGG
    CGCGGGGACA AATGTGAACT CCCCTGCCAG GATGGCACAT ATGGGCTGAA CTGTGCGGAG
    CGCTGTGACT GTAGCCATGC GGATGGCTGC CACTCCACCA CGGGCCACTG CCGCTGCCTC
    CCTGGATGGT CAGGTGTCCA CTGCGACAGC GTGTGTGCTG AGGGGCGCTG GGGGCCCAAC
    TGCTCTCTGC CCTGCTACTG TAAAAATGGG GCTTCATGCT CCCCAGACGA TGGCATCTGT
    GAGTGTGCGC CAGGGTTCCG AGGCACCACC TGTCAGAGAA TCTGCTCCCC TGGTTTTTAT
    GGGCATCGCT GCAGCCAGAC ATGCCCGCAG TGCGTGCATA GCAGTGGGCC CTGCCACCAC
    ATCACAGGCC TGTGTGACTG CTTGCCTGGC TTCACAGGTG CCCTCTGCAA TGAAGTGTGT
    CCCAGTGGCA GATTTGGGAA AAACTGTGCT GGAGTTTGTA CCTGTACAAA CAATGGAACC
    TGTAACCCCA TTGACCGATC TTGTCAGTGT TATCCAGGGT GGATTGGCAG TGACTGCTCG
    CAACCTTGTC CTCCTGCGCA TTGGGGTCCA AACTGCATCC ACACGTGCAA CTGCCACAAT
    GGGGCGTTCT GCAGTGCATA CGACGGGGAA TGTAAATGCA CTCCTGGCTG GACCGGACTC
    TACTGCACTC AGAGATGTCC CCTGGGTTTT TACGGAAAGG ACTGTGCGTT GATATGTCAG
    TGTCAGAATG GAGCTGACTG TGACCACATC TCTGGGCAGT GTACATGCCG CACTGGGTTC
    ATGGGGAAGC ACTGTGAACA GAAATGCCCT GCGGGAACAT ATGGCTATGG CTGTCGCCAG
    ATATGTGACT GTCTGAACAA CTCCACCTGT GACCATATCA CTGGGACTTG TTACTGTAGC
    CCAGGCTGGA AGGGGGCACG GTGTGATCAA GCTGGAGTCA TTATCGTTGG AAATCTGAAC
    AGCTTAAGCA GGACCAGTAC AGCTCTCCCT GCCAATTCCT ACCAGATCGG AGCCATCGTG
    GGCATCATCA TTCTTGTACT GGTTGTTCTC TTCCTCCTGG CGTTGTTCAT TATTTACAGA
    CAGAAGCAAA AGGGAAAGGA ATCAAGCATG CCTGCAGTTA CCTACACCCC TGCCCTGAGG
    GTCATGAATG CAGACTATAC CATTTCAGAA ACCCTTCCCC ACAGCAACAG TGGAAGTGCT
    AACAGCCATT ACTTCACTAA CCCCAGTTAC CACACACTTA CCCAGTGCGC CACATCCCCA
    CATGTCAACA ACAGGGACAG GATGACCATC GCAAAGTCAA AGAACAATCA GCTGTTTGTG
    AATCTGAAAA ATGTGAATCC TGGGAAGAGA GGCCCAGTGG CAGACTGCAC GGGAACATTG
    CCAGCAGACT GGAAACATGG CGGCTACCTT AATGAGCTTG GAGCTTTTGG ACTTGATAGA
    AGTTATATGG GAAAATCCTT GAAAGATTTG GGAAAGAATT CTGAATATAA TTCAAGTAAC
    TGCTCCTTAA GCAGTTCTGA AAACCCATAT GCCACTATTA AAGACCCGCC TGTACTTATC
    CCCAAAAACT CAGAGTGTGG CTATGTGGAA ATGAAGTCAC CAGCACGGAG AGATTCCCCC
    TATGCAGAGA TCAATAACTC AACTTCAGCC AACAAGAATG TCTATGAAGT TGAACCTACA
    GTGAGTGTTG TCCAAGGAAT ATTCAGCAAT AATGGGCATC TCACCCAGGA TCCATACGAC
    CTCCCAAAGA ACAGTCACAT CCCTTGCCAT TATGACCTGC TGCCAGTCCG AGACAGTTCA
    TCCTCCCCCA AGCGAGAGGA TGGTGGCAGT GGCAGCAGCA GCAGCAGTGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_531893.3
    CDS221..3634
    Translation

    Target ORF information:

    RefSeq Version XM_531893.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris multiple EGF-like-domains 10 (MEGF10), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_531893.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGTTGCTT CTTTGAACTC ATGCCTGAGC TTTATCTGTT TCTCATTATG CCTCTGGAGC 
    AGGACAGCAT CAGCACTGAA TCTTGAAGAC CCTAATGTGT GTAGCCACTG GGAAAGCTAC
    TCAGTGACTG TGCAAGAGTC ATACCCACAT CCTTTCGATC AAATCTACTA CACCAGCTGC
    ACTGATATTC TGAACTGGTT TAAATGTACA CGGCACAGAA TCAGTTATCG GACTGCCTAT
    CGACATGGGG AGAAAACTAT GTATAGGCGC AAATCCCAGT GTTGTCCTGG ATTTTATGAA
    AGCAGGGAAA TGTGTGTCCC CCACTGTGCT GATAAATGTG TCCATGGTCG CTGCATTGCT
    CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGTGGAACCA ACTGCTCCAG TGCCTGTGAT
    GGTGATCACT GGGGGCCCCA TTGCAGCAGC CGGTGCCAGT GCAAGAACGG GGCCCTGTGC
    AACCCCATCA CGGGGGCTTG CCACTGCGCT GCAGGTTTCC GGGGCTGGCG TTGTGAGGAC
    CGCTGTGAGC AGGGCACCTA CGGTAATGAC TGTCACCAGA GATGCCAGTG TCAGAATGGA
    GCCACCTGCG ACCACGTCAC AGGGGAATGC CGCTGCCCAC CAGGGTACAC TGGAGCCTTC
    TGTGAGGACC TTTGTCCCCC TGGCAAGCAC GGTCCGCAGT GCGAGCAGAG ATGTCCCTGC
    CAAAATGGAG GAATATGTCA TCACGTCACC GGAGAATGCT CTTGCCCTTC GGGCTGGCTG
    GGCACAGTCT GTGGTCAGCC TTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCACAAGAA
    TGTCAGTGCC ATAATGGAGG GACGTGTGAT GCTGCCACAG GCCAATGTCA TTGCAGTCCA
    GGATACACAG GGGAACGCTG CCAGGACGAG TGTCCCGTTG GCACATACGG AGTTCGCTGT
    GCTGAGACCT GCCAGTGTGT CAATGGAGGG AAGTGTTATC ACGTGAGTGG CTCATGCCTC
    TGTGAAGCAG GCTTTGCTGG TGAGCACTGT GAGGCACGCC TGTGTCCCGA AGGGCTCTAC
    GGCATCAAAT GTGACAAGCG GTGTCCCTGC CACTTGGACA ACACTCATAG CTGTCACCCC
    ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC TGGTCAGGAC TCTACTGTAA CGAAACATGT
    TCTCCTGGAT TCTTTGGGGA AGCTTGCCAA CAGATCTGCA GCTGCCACAA CGGGGCTGAT
    TGTGACAGTG TGACTGGAAA ATGCATCTGT GCCCCAGGAT TCAAAGGAAT TGACTGCTCT
    ATCCCATGCC CATTGGAAAC CTATGGGATA AACTGCTCCT CTCTCTGTGG CTGTAAAAAC
    GATGCTGTCT GTTCACCTGT AGATGGGTCT TGTACTTGCA AGGCAGGCTG GCACGGGGTG
    GACTGCTCCA TCAGCTGTCC CAGTGGCACG TGGGGCTTCA GCTGTAACCT GACCTGCCAG
    TGCCTCCACG GGGGAGCCTG CAACACCCAG GACGGGACCT GCACGTGTGC GCCTGGATGG
    CGCGGGGACA AATGTGAACT CCCCTGCCAG GATGGCACAT ATGGGCTGAA CTGTGCGGAG
    CGCTGTGACT GTAGCCATGC GGATGGCTGC CACTCCACCA CGGGCCACTG CCGCTGCCTC
    CCTGGATGGT CAGGTGTCCA CTGCGACAGC GTGTGTGCTG AGGGGCGCTG GGGGCCCAAC
    TGCTCTCTGC CCTGCTACTG TAAAAATGGG GCTTCATGCT CCCCAGACGA TGGCATCTGT
    GAGTGTGCGC CAGGGTTCCG AGGCACCACC TGTCAGAGAA TCTGCTCCCC TGGTTTTTAT
    GGGCATCGCT GCAGCCAGAC ATGCCCGCAG TGCGTGCATA GCAGTGGGCC CTGCCACCAC
    ATCACAGGCC TGTGTGACTG CTTGCCTGGC TTCACAGGTG CCCTCTGCAA TGAAGTGTGT
    CCCAGTGGCA GATTTGGGAA AAACTGTGCT GGAGTTTGTA CCTGTACAAA CAATGGAACC
    TGTAACCCCA TTGACCGATC TTGTCAGTGT TATCCAGGGT GGATTGGCAG TGACTGCTCG
    CAACCTTGTC CTCCTGCGCA TTGGGGTCCA AACTGCATCC ACACGTGCAA CTGCCACAAT
    GGGGCGTTCT GCAGTGCATA CGACGGGGAA TGTAAATGCA CTCCTGGCTG GACCGGACTC
    TACTGCACTC AGAGATGTCC CCTGGGTTTT TACGGAAAGG ACTGTGCGTT GATATGTCAG
    TGTCAGAATG GAGCTGACTG TGACCACATC TCTGGGCAGT GTACATGCCG CACTGGGTTC
    ATGGGGAAGC ACTGTGAACA GAAATGCCCT GCGGGAACAT ATGGCTATGG CTGTCGCCAG
    ATATGTGACT GTCTGAACAA CTCCACCTGT GACCATATCA CTGGGACTTG TTACTGTAGC
    CCAGGCTGGA AGGGGGCACG GTGTGATCAA GCTGGAGTCA TTATCGTTGG AAATCTGAAC
    AGCTTAAGCA GGACCAGTAC AGCTCTCCCT GCCAATTCCT ACCAGATCGG AGCCATCGTG
    GGCATCATCA TTCTTGTACT GGTTGTTCTC TTCCTCCTGG CGTTGTTCAT TATTTACAGA
    CAGAAGCAAA AGGGAAAGGA ATCAAGCATG CCTGCAGTTA CCTACACCCC TGCCCTGAGG
    GTCATGAATG CAGACTATAC CATTTCAGAA ACCCTTCCCC ACAGCAACAG TGGAAGTGCT
    AACAGCCATT ACTTCACTAA CCCCAGTTAC CACACACTTA CCCAGTGCGC CACATCCCCA
    CATGTCAACA ACAGGGACAG GATGACCATC GCAAAGTCAA AGAACAATCA GCTGTTTGTG
    AATCTGAAAA ATGTGAATCC TGGGAAGAGA GGCCCAGTGG CAGACTGCAC GGGAACATTG
    CCAGCAGACT GGAAACATGG CGGCTACCTT AATGAGCTTG GAGCTTTTGG ACTTGATAGA
    AGTTATATGG GAAAATCCTT GAAAGATTTG GGAAAGAATT CTGAATATAA TTCAAGTAAC
    TGCTCCTTAA GCAGTTCTGA AAACCCATAT GCCACTATTA AAGACCCGCC TGTACTTATC
    CCCAAAAACT CAGAGTGTGG CTATGTGGAA ATGAAGTCAC CAGCACGGAG AGATTCCCCC
    TATGCAGAGA TCAATAACTC AACTTCAGCC AACAAGAATG TCTATGAAGT TGAACCTACA
    GTGAGTGTTG TCCAAGGAAT ATTCAGCAAT AATGGGCATC TCACCCAGGA TCCATACGAC
    CTCCCAAAGA ACAGTCACAT CCCTTGCCAT TATGACCTGC TGCCAGTCCG AGACAGTTCA
    TCCTCCCCCA AGCGAGAGGA TGGTGGCAGT GGCAGCAGCA GCAGCAGTGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe14211
    Clone ID Related Accession (Same CDS sequence) XM_014117692.1 , XM_014117690.2 , XM_005626476.2 , XM_531893.6 , XM_014117690.1 , XM_014117691.1 , XM_005626476.3 , XM_531893.5 , XM_014117691.2
    Accession Version XM_014117691.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3414bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product multiple epidermal growth factor-like domains protein 10 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014117691.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGTTGCTT CTTTGAACTC ATGCCTGAGC TTTATCTGTT TCTCATTATG CCTCTGGAGC 
    AGGACAGCAT CAGCACTGAA TCTTGAAGAC CCTAATGTGT GTAGCCACTG GGAAAGCTAC
    TCAGTGACTG TGCAAGAGTC ATACCCACAT CCTTTCGATC AAATCTACTA CACCAGCTGC
    ACTGATATTC TGAACTGGTT TAAATGTACA CGGCACAGAA TCAGTTATCG GACTGCCTAT
    CGACATGGGG AGAAAACTAT GTATAGGCGC AAATCCCAGT GTTGTCCTGG ATTTTATGAA
    AGCAGGGAAA TGTGTGTCCC CCACTGTGCT GATAAATGTG TCCATGGTCG CTGCATTGCT
    CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGTGGAACCA ACTGCTCCAG TGCCTGTGAT
    GGTGATCACT GGGGGCCCCA TTGCAGCAGC CGGTGCCAGT GCAAGAACGG GGCCCTGTGC
    AACCCCATCA CGGGGGCTTG CCACTGCGCT GCAGGTTTCC GGGGCTGGCG TTGTGAGGAC
    CGCTGTGAGC AGGGCACCTA CGGTAATGAC TGTCACCAGA GATGCCAGTG TCAGAATGGA
    GCCACCTGCG ACCACGTCAC AGGGGAATGC CGCTGCCCAC CAGGGTACAC TGGAGCCTTC
    TGTGAGGACC TTTGTCCCCC TGGCAAGCAC GGTCCGCAGT GCGAGCAGAG ATGTCCCTGC
    CAAAATGGAG GAATATGTCA TCACGTCACC GGAGAATGCT CTTGCCCTTC GGGCTGGCTG
    GGCACAGTCT GTGGTCAGCC TTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCACAAGAA
    TGTCAGTGCC ATAATGGAGG GACGTGTGAT GCTGCCACAG GCCAATGTCA TTGCAGTCCA
    GGATACACAG GGGAACGCTG CCAGGACGAG TGTCCCGTTG GCACATACGG AGTTCGCTGT
    GCTGAGACCT GCCAGTGTGT CAATGGAGGG AAGTGTTATC ACGTGAGTGG CTCATGCCTC
    TGTGAAGCAG GCTTTGCTGG TGAGCACTGT GAGGCACGCC TGTGTCCCGA AGGGCTCTAC
    GGCATCAAAT GTGACAAGCG GTGTCCCTGC CACTTGGACA ACACTCATAG CTGTCACCCC
    ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC TGGTCAGGAC TCTACTGTAA CGAAACATGT
    TCTCCTGGAT TCTTTGGGGA AGCTTGCCAA CAGATCTGCA GCTGCCACAA CGGGGCTGAT
    TGTGACAGTG TGACTGGAAA ATGCATCTGT GCCCCAGGAT TCAAAGGAAT TGACTGCTCT
    ATCCCATGCC CATTGGAAAC CTATGGGATA AACTGCTCCT CTCTCTGTGG CTGTAAAAAC
    GATGCTGTCT GTTCACCTGT AGATGGGTCT TGTACTTGCA AGGCAGGCTG GCACGGGGTG
    GACTGCTCCA TCAGCTGTCC CAGTGGCACG TGGGGCTTCA GCTGTAACCT GACCTGCCAG
    TGCCTCCACG GGGGAGCCTG CAACACCCAG GACGGGACCT GCACGTGTGC GCCTGGATGG
    CGCGGGGACA AATGTGAACT CCCCTGCCAG GATGGCACAT ATGGGCTGAA CTGTGCGGAG
    CGCTGTGACT GTAGCCATGC GGATGGCTGC CACTCCACCA CGGGCCACTG CCGCTGCCTC
    CCTGGATGGT CAGGTGTCCA CTGCGACAGC GTGTGTGCTG AGGGGCGCTG GGGGCCCAAC
    TGCTCTCTGC CCTGCTACTG TAAAAATGGG GCTTCATGCT CCCCAGACGA TGGCATCTGT
    GAGTGTGCGC CAGGGTTCCG AGGCACCACC TGTCAGAGAA TCTGCTCCCC TGGTTTTTAT
    GGGCATCGCT GCAGCCAGAC ATGCCCGCAG TGCGTGCATA GCAGTGGGCC CTGCCACCAC
    ATCACAGGCC TGTGTGACTG CTTGCCTGGC TTCACAGGTG CCCTCTGCAA TGAAGTGTGT
    CCCAGTGGCA GATTTGGGAA AAACTGTGCT GGAGTTTGTA CCTGTACAAA CAATGGAACC
    TGTAACCCCA TTGACCGATC TTGTCAGTGT TATCCAGGGT GGATTGGCAG TGACTGCTCG
    CAACCTTGTC CTCCTGCGCA TTGGGGTCCA AACTGCATCC ACACGTGCAA CTGCCACAAT
    GGGGCGTTCT GCAGTGCATA CGACGGGGAA TGTAAATGCA CTCCTGGCTG GACCGGACTC
    TACTGCACTC AGAGATGTCC CCTGGGTTTT TACGGAAAGG ACTGTGCGTT GATATGTCAG
    TGTCAGAATG GAGCTGACTG TGACCACATC TCTGGGCAGT GTACATGCCG CACTGGGTTC
    ATGGGGAAGC ACTGTGAACA GAAATGCCCT GCGGGAACAT ATGGCTATGG CTGTCGCCAG
    ATATGTGACT GTCTGAACAA CTCCACCTGT GACCATATCA CTGGGACTTG TTACTGTAGC
    CCAGGCTGGA AGGGGGCACG GTGTGATCAA GCTGGAGTCA TTATCGTTGG AAATCTGAAC
    AGCTTAAGCA GGACCAGTAC AGCTCTCCCT GCCAATTCCT ACCAGATCGG AGCCATCGTG
    GGCATCATCA TTCTTGTACT GGTTGTTCTC TTCCTCCTGG CGTTGTTCAT TATTTACAGA
    CAGAAGCAAA AGGGAAAGGA ATCAAGCATG CCTGCAGTTA CCTACACCCC TGCCCTGAGG
    GTCATGAATG CAGACTATAC CATTTCAGAA ACCCTTCCCC ACAGCAACAG TGGAAGTGCT
    AACAGCCATT ACTTCACTAA CCCCAGTTAC CACACACTTA CCCAGTGCGC CACATCCCCA
    CATGTCAACA ACAGGGACAG GATGACCATC GCAAAGTCAA AGAACAATCA GCTGTTTGTG
    AATCTGAAAA ATGTGAATCC TGGGAAGAGA GGCCCAGTGG CAGACTGCAC GGGAACATTG
    CCAGCAGACT GGAAACATGG CGGCTACCTT AATGAGCTTG GAGCTTTTGG ACTTGATAGA
    AGTTATATGG GAAAATCCTT GAAAGATTTG GGAAAGAATT CTGAATATAA TTCAAGTAAC
    TGCTCCTTAA GCAGTTCTGA AAACCCATAT GCCACTATTA AAGACCCGCC TGTACTTATC
    CCCAAAAACT CAGAGTGTGG CTATGTGGAA ATGAAGTCAC CAGCACGGAG AGATTCCCCC
    TATGCAGAGA TCAATAACTC AACTTCAGCC AACAAGAATG TCTATGAAGT TGAACCTACA
    GTGAGTGTTG TCCAAGGAAT ATTCAGCAAT AATGGGCATC TCACCCAGGA TCCATACGAC
    CTCCCAAAGA ACAGTCACAT CCCTTGCCAT TATGACCTGC TGCCAGTCCG AGACAGTTCA
    TCCTCCCCCA AGCGAGAGGA TGGTGGCAGT GGCAGCAGCA GCAGCAGTGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973166.1
    CDS143..3556
    Translation

    Target ORF information:

    RefSeq Version XM_014117691.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris multiple EGF like domains 10 (MEGF10), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014117691.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGTTGCTT CTTTGAACTC ATGCCTGAGC TTTATCTGTT TCTCATTATG CCTCTGGAGC 
    AGGACAGCAT CAGCACTGAA TCTTGAAGAC CCTAATGTGT GTAGCCACTG GGAAAGCTAC
    TCAGTGACTG TGCAAGAGTC ATACCCACAT CCTTTCGATC AAATCTACTA CACCAGCTGC
    ACTGATATTC TGAACTGGTT TAAATGTACA CGGCACAGAA TCAGTTATCG GACTGCCTAT
    CGACATGGGG AGAAAACTAT GTATAGGCGC AAATCCCAGT GTTGTCCTGG ATTTTATGAA
    AGCAGGGAAA TGTGTGTCCC CCACTGTGCT GATAAATGTG TCCATGGTCG CTGCATTGCT
    CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGTGGAACCA ACTGCTCCAG TGCCTGTGAT
    GGTGATCACT GGGGGCCCCA TTGCAGCAGC CGGTGCCAGT GCAAGAACGG GGCCCTGTGC
    AACCCCATCA CGGGGGCTTG CCACTGCGCT GCAGGTTTCC GGGGCTGGCG TTGTGAGGAC
    CGCTGTGAGC AGGGCACCTA CGGTAATGAC TGTCACCAGA GATGCCAGTG TCAGAATGGA
    GCCACCTGCG ACCACGTCAC AGGGGAATGC CGCTGCCCAC CAGGGTACAC TGGAGCCTTC
    TGTGAGGACC TTTGTCCCCC TGGCAAGCAC GGTCCGCAGT GCGAGCAGAG ATGTCCCTGC
    CAAAATGGAG GAATATGTCA TCACGTCACC GGAGAATGCT CTTGCCCTTC GGGCTGGCTG
    GGCACAGTCT GTGGTCAGCC TTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCACAAGAA
    TGTCAGTGCC ATAATGGAGG GACGTGTGAT GCTGCCACAG GCCAATGTCA TTGCAGTCCA
    GGATACACAG GGGAACGCTG CCAGGACGAG TGTCCCGTTG GCACATACGG AGTTCGCTGT
    GCTGAGACCT GCCAGTGTGT CAATGGAGGG AAGTGTTATC ACGTGAGTGG CTCATGCCTC
    TGTGAAGCAG GCTTTGCTGG TGAGCACTGT GAGGCACGCC TGTGTCCCGA AGGGCTCTAC
    GGCATCAAAT GTGACAAGCG GTGTCCCTGC CACTTGGACA ACACTCATAG CTGTCACCCC
    ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC TGGTCAGGAC TCTACTGTAA CGAAACATGT
    TCTCCTGGAT TCTTTGGGGA AGCTTGCCAA CAGATCTGCA GCTGCCACAA CGGGGCTGAT
    TGTGACAGTG TGACTGGAAA ATGCATCTGT GCCCCAGGAT TCAAAGGAAT TGACTGCTCT
    ATCCCATGCC CATTGGAAAC CTATGGGATA AACTGCTCCT CTCTCTGTGG CTGTAAAAAC
    GATGCTGTCT GTTCACCTGT AGATGGGTCT TGTACTTGCA AGGCAGGCTG GCACGGGGTG
    GACTGCTCCA TCAGCTGTCC CAGTGGCACG TGGGGCTTCA GCTGTAACCT GACCTGCCAG
    TGCCTCCACG GGGGAGCCTG CAACACCCAG GACGGGACCT GCACGTGTGC GCCTGGATGG
    CGCGGGGACA AATGTGAACT CCCCTGCCAG GATGGCACAT ATGGGCTGAA CTGTGCGGAG
    CGCTGTGACT GTAGCCATGC GGATGGCTGC CACTCCACCA CGGGCCACTG CCGCTGCCTC
    CCTGGATGGT CAGGTGTCCA CTGCGACAGC GTGTGTGCTG AGGGGCGCTG GGGGCCCAAC
    TGCTCTCTGC CCTGCTACTG TAAAAATGGG GCTTCATGCT CCCCAGACGA TGGCATCTGT
    GAGTGTGCGC CAGGGTTCCG AGGCACCACC TGTCAGAGAA TCTGCTCCCC TGGTTTTTAT
    GGGCATCGCT GCAGCCAGAC ATGCCCGCAG TGCGTGCATA GCAGTGGGCC CTGCCACCAC
    ATCACAGGCC TGTGTGACTG CTTGCCTGGC TTCACAGGTG CCCTCTGCAA TGAAGTGTGT
    CCCAGTGGCA GATTTGGGAA AAACTGTGCT GGAGTTTGTA CCTGTACAAA CAATGGAACC
    TGTAACCCCA TTGACCGATC TTGTCAGTGT TATCCAGGGT GGATTGGCAG TGACTGCTCG
    CAACCTTGTC CTCCTGCGCA TTGGGGTCCA AACTGCATCC ACACGTGCAA CTGCCACAAT
    GGGGCGTTCT GCAGTGCATA CGACGGGGAA TGTAAATGCA CTCCTGGCTG GACCGGACTC
    TACTGCACTC AGAGATGTCC CCTGGGTTTT TACGGAAAGG ACTGTGCGTT GATATGTCAG
    TGTCAGAATG GAGCTGACTG TGACCACATC TCTGGGCAGT GTACATGCCG CACTGGGTTC
    ATGGGGAAGC ACTGTGAACA GAAATGCCCT GCGGGAACAT ATGGCTATGG CTGTCGCCAG
    ATATGTGACT GTCTGAACAA CTCCACCTGT GACCATATCA CTGGGACTTG TTACTGTAGC
    CCAGGCTGGA AGGGGGCACG GTGTGATCAA GCTGGAGTCA TTATCGTTGG AAATCTGAAC
    AGCTTAAGCA GGACCAGTAC AGCTCTCCCT GCCAATTCCT ACCAGATCGG AGCCATCGTG
    GGCATCATCA TTCTTGTACT GGTTGTTCTC TTCCTCCTGG CGTTGTTCAT TATTTACAGA
    CAGAAGCAAA AGGGAAAGGA ATCAAGCATG CCTGCAGTTA CCTACACCCC TGCCCTGAGG
    GTCATGAATG CAGACTATAC CATTTCAGAA ACCCTTCCCC ACAGCAACAG TGGAAGTGCT
    AACAGCCATT ACTTCACTAA CCCCAGTTAC CACACACTTA CCCAGTGCGC CACATCCCCA
    CATGTCAACA ACAGGGACAG GATGACCATC GCAAAGTCAA AGAACAATCA GCTGTTTGTG
    AATCTGAAAA ATGTGAATCC TGGGAAGAGA GGCCCAGTGG CAGACTGCAC GGGAACATTG
    CCAGCAGACT GGAAACATGG CGGCTACCTT AATGAGCTTG GAGCTTTTGG ACTTGATAGA
    AGTTATATGG GAAAATCCTT GAAAGATTTG GGAAAGAATT CTGAATATAA TTCAAGTAAC
    TGCTCCTTAA GCAGTTCTGA AAACCCATAT GCCACTATTA AAGACCCGCC TGTACTTATC
    CCCAAAAACT CAGAGTGTGG CTATGTGGAA ATGAAGTCAC CAGCACGGAG AGATTCCCCC
    TATGCAGAGA TCAATAACTC AACTTCAGCC AACAAGAATG TCTATGAAGT TGAACCTACA
    GTGAGTGTTG TCCAAGGAAT ATTCAGCAAT AATGGGCATC TCACCCAGGA TCCATACGAC
    CTCCCAAAGA ACAGTCACAT CCCTTGCCAT TATGACCTGC TGCCAGTCCG AGACAGTTCA
    TCCTCCCCCA AGCGAGAGGA TGGTGGCAGT GGCAGCAGCA GCAGCAGTGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe14212
    Clone ID Related Accession (Same CDS sequence) XM_022425234.1
    Accession Version XM_022425234.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3303bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product multiple epidermal growth factor-like domains protein 10 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGTTGCTT CTTTGAACTC ATGCCTGAGC TTTATCTGTT TCTCATTATG CCTCTGGAGC 
    AGGACAGCAT CAGCACTGAA TCTTGAAGAC CCTAATGTGT GTAGCCACTG GGAAAGCTAC
    TCAGTGACTG TGCAAGAGTC ATACCCACAT CCTTTCGATC AAATCTACTA CACCAGCTGC
    ACTGATATTC TGAACTGGTT TAAATGTACA CGGCACAGAA TCAGTTATCG GACTGCCTAT
    CGACATGGGG AGAAAACTAT GTATAGGCGC AAATCCCAGT GTTGTCCTGG ATTTTATGAA
    AGCAGGGAAA TGTGTGTCCC CCACTGTGCT GATAAATGTG TCCATGGTCG CTGCATTGCT
    CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGTGGAACCA ACTGCTCCAG TGCCTGTGAT
    GGTGATCACT GGGGGCCCCA TTGCAGCAGC CGGTGCCAGT GCAAGAACGG GGCCCTGTGC
    AACCCCATCA CGGGGGCTTG CCACTGCGCT GCAGGTTTCC GGGGCTGGCG TTGTGAGGAC
    CGCTGTGAGC AGGGCACCTA CGGTAATGAC TGTCACCAGA GATGCCAGTG TCAGAATGGA
    GCCACCTGCG ACCACGTCAC AGGGGAATGC CGCTGCCCAC CAGGGTACAC TGGAGCCTTC
    TGTGAGGACC TTTGTCCCCC TGGCAAGCAC GGTCCGCAGT GCGAGCAGAG ATGTCCCTGC
    CAAAATGGAG GAATATGTCA TCACGTCACC GGAGAATGCT CTTGCCCTTC GGGCTGGCTG
    GGCACAGTCT GTGGTCAGCC TTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCACAAGAA
    TGTCAGTGCC ATAATGGAGG GACGTGTGAT GCTGCCACAG GCCAATGTCA TTGCAGTCCA
    GGATACACAG GGGAACGCTG CCAGGACGAG TGTCCCGTTG GCACATACGG AGTTCGCTGT
    GCTGAGACCT GCCAGTGTGT CAATGGAGGG AAGTGTTATC ACGTGAGTGG CTCATGCCTC
    TGTGAAGCAG GCTTTGCTGG TGAGCACTGT GAGGCACGCC TGTGTCCCGA AGGGCTCTAC
    GGCATCAAAT GTGACAAGCG GTGTCCCTGC CACTTGGACA ACACTCATAG CTGTCACCCC
    ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC TGGTCAGGAC TCTACTGTAA CGAAACATGT
    TCTCCTGGAT TCTTTGGGGA AGCTTGCCAA CAGATCTGCA GCTGCCACAA CGGGGCTGAT
    TGTGACAGTG TGACTGGAAA ATGCATCTGT GCCCCAGGAT TCAAAGGAAT TGACTGCTCT
    ATCCCATGCC CATTGGAAAC CTATGGGATA AACTGCTCCT CTCTCTGTGG CTGTAAAAAC
    GATGCTGTCT GTTCACCTGT AGATGGGTCT TGTACTTGCA AGGCAGGCTG GCACGGGGTG
    GACTGCTCCA TCAGCTGTCC CAGTGGCACG TGGGGCTTCA GCTGTAACCT GACCTGCCAG
    TGCCTCCACG GGGGAGCCTG CAACACCCAG GACGGGACCT GCACGTGTGC GCCTGGATGG
    CGCGGGGACA AATGTGAACT CCCCTGCCAG GATGGCACAT ATGGGCTGAA CTGTGCGGAG
    CGCTGTGACT GTAGCCATGC GGATGGCTGC CACTCCACCA CGGGCCACTG CCGCTGCCTC
    CCTGGATGGT CAGGTGTCCA CTGCGACAGC GTGTGTGCTG AGGGGCGCTG GGGGCCCAAC
    TGCTCTCTGC CCTGCTACTG TAAAAATGGG GCTTCATGCT CCCCAGACGA TGGCATCTGT
    GAGTGTGCGC CAGGGTTCCG AGGCACCACC TGTCAGAGAA TCTGCTCCCC TGGTTTTTAT
    GGGCATCGCT GCAGCCAGAC ATGCCCGCAG TGCGTGCATA GCAGTGGGCC CTGCCACCAC
    ATCACAGGCC TGTGTGACTG CTTGCCTGGC TTCACAGGTG CCCTCTGCAA TGAAGTGTGT
    CCCAGTGGCA GATTTGGGAA AAACTGTGCT GGAGTTTGTA CCTGTACAAA CAATGGAACC
    TGTAACCCCA TTGACCGATC TTGTCAGTGT TATCCAGGGT GGATTGGCAG TGACTGCTCG
    CAACCTTGTC CTCCTGCGCA TTGGGGTCCA AACTGCATCC ACACGTGCAA CTGCCACAAT
    GGGGCGTTCT GCAGTGCATA CGACGGGGAA TGTAAATGCA CTCCTGGCTG GACCGGACTC
    TACTGCACTC AGAGATGTCC CCTGGGTTTT TACGGAAAGG ACTGTGCGTT GATATGTCAG
    TGTCAGAATG GAGCTGACTG TGACCACATC TCTGGGCAGT GTACATGCCG CACTGGGTTC
    ATGGGGAAGC ACTGTGAACA GAAATGCCCT GCGGGAACAT ATGGCTATGG CTGTCGCCAG
    ATATGTGACT GTCTGAACAA CTCCACCTGT GACCATATCA CTGGGACTTG TTACTGTAGC
    CCAGGCTGGA AGGGGGCACG GTGTGATCAA GCTGGAGTCA TTATCGTTGG AAATCTGAAC
    AGCTTAAGCA GGACCAGTAC AGCTCTCCCT GCCAATTCCT ACCAGATCGG AGCCATCGTG
    GGCATCATCA TTCTTGTACT GGTTGTTCTC TTCCTCCTGG CGTTGTTCAT TATTTACAGA
    CAGAAGCAAA AGGGAAAGGA ATCAAGCATG CCTGCAGTTA CCTACACCCC TGCCCTGAGG
    GTCATGAATG CAGACTATAC CATTTCAGAA ACCCTTCCCC ACAGCAACAG TGGAAGTGCT
    AACAGCCATT ACTTCACTAA CCCCAGTTAC CACACACTTA CCCAGTGCGC CACATCCCCA
    CATGTCAACA ACAGGGACAG GATGACCATC GCAAAGTCAA AGAACAATCA GCTGTTTGTG
    AATCTGAAAA ATGTGAATCC TGGGAAGAGA GGCCCAGTGG CAGACTGCAC GGGAACATTG
    CCAGCAGACT GGAAACATGG CGGCTACCTT AATGAGCTTG GAGCTTTTGG ACTTGATAGA
    AGTTATATGG GAAAATCCTT GAAAGATTTG GGAAAGAATT CTGAATATAA TTCAAGTAAC
    TGCTCCTTAA GCAGTTCTGA AAACCCATAT GCCACTATTA AAGACCCGCC TGTACTTATC
    CCCAAAAACT CAGAGTGTGG CTATGTGGAA ATGAAGTCAC CAGCACGGAG AGATTCCCCC
    TATGCAGAGA TCAATAACTC AACTTCAGCC AACAAGAATG TCTATGAAGT TGGTGCATCT
    TGGACACTGG GACAATTAGG AGAACAGACA GGAACAGTGT CCTCTCTGAA GTTCCAGCCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022280942.1
    CDS818..4120
    Translation

    Target ORF information:

    RefSeq Version XM_022425234.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris multiple EGF like domains 10 (MEGF10), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022425234.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGTTGCTT CTTTGAACTC ATGCCTGAGC TTTATCTGTT TCTCATTATG CCTCTGGAGC 
    AGGACAGCAT CAGCACTGAA TCTTGAAGAC CCTAATGTGT GTAGCCACTG GGAAAGCTAC
    TCAGTGACTG TGCAAGAGTC ATACCCACAT CCTTTCGATC AAATCTACTA CACCAGCTGC
    ACTGATATTC TGAACTGGTT TAAATGTACA CGGCACAGAA TCAGTTATCG GACTGCCTAT
    CGACATGGGG AGAAAACTAT GTATAGGCGC AAATCCCAGT GTTGTCCTGG ATTTTATGAA
    AGCAGGGAAA TGTGTGTCCC CCACTGTGCT GATAAATGTG TCCATGGTCG CTGCATTGCT
    CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGTGGAACCA ACTGCTCCAG TGCCTGTGAT
    GGTGATCACT GGGGGCCCCA TTGCAGCAGC CGGTGCCAGT GCAAGAACGG GGCCCTGTGC
    AACCCCATCA CGGGGGCTTG CCACTGCGCT GCAGGTTTCC GGGGCTGGCG TTGTGAGGAC
    CGCTGTGAGC AGGGCACCTA CGGTAATGAC TGTCACCAGA GATGCCAGTG TCAGAATGGA
    GCCACCTGCG ACCACGTCAC AGGGGAATGC CGCTGCCCAC CAGGGTACAC TGGAGCCTTC
    TGTGAGGACC TTTGTCCCCC TGGCAAGCAC GGTCCGCAGT GCGAGCAGAG ATGTCCCTGC
    CAAAATGGAG GAATATGTCA TCACGTCACC GGAGAATGCT CTTGCCCTTC GGGCTGGCTG
    GGCACAGTCT GTGGTCAGCC TTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCACAAGAA
    TGTCAGTGCC ATAATGGAGG GACGTGTGAT GCTGCCACAG GCCAATGTCA TTGCAGTCCA
    GGATACACAG GGGAACGCTG CCAGGACGAG TGTCCCGTTG GCACATACGG AGTTCGCTGT
    GCTGAGACCT GCCAGTGTGT CAATGGAGGG AAGTGTTATC ACGTGAGTGG CTCATGCCTC
    TGTGAAGCAG GCTTTGCTGG TGAGCACTGT GAGGCACGCC TGTGTCCCGA AGGGCTCTAC
    GGCATCAAAT GTGACAAGCG GTGTCCCTGC CACTTGGACA ACACTCATAG CTGTCACCCC
    ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC TGGTCAGGAC TCTACTGTAA CGAAACATGT
    TCTCCTGGAT TCTTTGGGGA AGCTTGCCAA CAGATCTGCA GCTGCCACAA CGGGGCTGAT
    TGTGACAGTG TGACTGGAAA ATGCATCTGT GCCCCAGGAT TCAAAGGAAT TGACTGCTCT
    ATCCCATGCC CATTGGAAAC CTATGGGATA AACTGCTCCT CTCTCTGTGG CTGTAAAAAC
    GATGCTGTCT GTTCACCTGT AGATGGGTCT TGTACTTGCA AGGCAGGCTG GCACGGGGTG
    GACTGCTCCA TCAGCTGTCC CAGTGGCACG TGGGGCTTCA GCTGTAACCT GACCTGCCAG
    TGCCTCCACG GGGGAGCCTG CAACACCCAG GACGGGACCT GCACGTGTGC GCCTGGATGG
    CGCGGGGACA AATGTGAACT CCCCTGCCAG GATGGCACAT ATGGGCTGAA CTGTGCGGAG
    CGCTGTGACT GTAGCCATGC GGATGGCTGC CACTCCACCA CGGGCCACTG CCGCTGCCTC
    CCTGGATGGT CAGGTGTCCA CTGCGACAGC GTGTGTGCTG AGGGGCGCTG GGGGCCCAAC
    TGCTCTCTGC CCTGCTACTG TAAAAATGGG GCTTCATGCT CCCCAGACGA TGGCATCTGT
    GAGTGTGCGC CAGGGTTCCG AGGCACCACC TGTCAGAGAA TCTGCTCCCC TGGTTTTTAT
    GGGCATCGCT GCAGCCAGAC ATGCCCGCAG TGCGTGCATA GCAGTGGGCC CTGCCACCAC
    ATCACAGGCC TGTGTGACTG CTTGCCTGGC TTCACAGGTG CCCTCTGCAA TGAAGTGTGT
    CCCAGTGGCA GATTTGGGAA AAACTGTGCT GGAGTTTGTA CCTGTACAAA CAATGGAACC
    TGTAACCCCA TTGACCGATC TTGTCAGTGT TATCCAGGGT GGATTGGCAG TGACTGCTCG
    CAACCTTGTC CTCCTGCGCA TTGGGGTCCA AACTGCATCC ACACGTGCAA CTGCCACAAT
    GGGGCGTTCT GCAGTGCATA CGACGGGGAA TGTAAATGCA CTCCTGGCTG GACCGGACTC
    TACTGCACTC AGAGATGTCC CCTGGGTTTT TACGGAAAGG ACTGTGCGTT GATATGTCAG
    TGTCAGAATG GAGCTGACTG TGACCACATC TCTGGGCAGT GTACATGCCG CACTGGGTTC
    ATGGGGAAGC ACTGTGAACA GAAATGCCCT GCGGGAACAT ATGGCTATGG CTGTCGCCAG
    ATATGTGACT GTCTGAACAA CTCCACCTGT GACCATATCA CTGGGACTTG TTACTGTAGC
    CCAGGCTGGA AGGGGGCACG GTGTGATCAA GCTGGAGTCA TTATCGTTGG AAATCTGAAC
    AGCTTAAGCA GGACCAGTAC AGCTCTCCCT GCCAATTCCT ACCAGATCGG AGCCATCGTG
    GGCATCATCA TTCTTGTACT GGTTGTTCTC TTCCTCCTGG CGTTGTTCAT TATTTACAGA
    CAGAAGCAAA AGGGAAAGGA ATCAAGCATG CCTGCAGTTA CCTACACCCC TGCCCTGAGG
    GTCATGAATG CAGACTATAC CATTTCAGAA ACCCTTCCCC ACAGCAACAG TGGAAGTGCT
    AACAGCCATT ACTTCACTAA CCCCAGTTAC CACACACTTA CCCAGTGCGC CACATCCCCA
    CATGTCAACA ACAGGGACAG GATGACCATC GCAAAGTCAA AGAACAATCA GCTGTTTGTG
    AATCTGAAAA ATGTGAATCC TGGGAAGAGA GGCCCAGTGG CAGACTGCAC GGGAACATTG
    CCAGCAGACT GGAAACATGG CGGCTACCTT AATGAGCTTG GAGCTTTTGG ACTTGATAGA
    AGTTATATGG GAAAATCCTT GAAAGATTTG GGAAAGAATT CTGAATATAA TTCAAGTAAC
    TGCTCCTTAA GCAGTTCTGA AAACCCATAT GCCACTATTA AAGACCCGCC TGTACTTATC
    CCCAAAAACT CAGAGTGTGG CTATGTGGAA ATGAAGTCAC CAGCACGGAG AGATTCCCCC
    TATGCAGAGA TCAATAACTC AACTTCAGCC AACAAGAATG TCTATGAAGT TGGTGCATCT
    TGGACACTGG GACAATTAGG AGAACAGACA GGAACAGTGT CCTCTCTGAA GTTCCAGCCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe14213
    Clone ID Related Accession (Same CDS sequence) XM_005626478.3 , XM_005626478.2
    Accession Version XM_005626478.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3264bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product multiple epidermal growth factor-like domains protein 10 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005626478.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGTTGCTT CTTTGAACTC ATGCCTGAGC TTTATCTGTT TCTCATTATG CCTCTGGAGC 
    AGGACAGCAT CAGCACTGAA TCTTGAAGAC CCTAATGTGT GTAGCCACTG GGAAAGCTAC
    TCAGTGACTG TGCAAGAGTC ATACCCACAT CCTTTCGATC AAATCTACTA CACCAGCTGC
    ACTGATATTC TGAACTGGTT TAAATGTACA CGGCACAGAA TCAGTTATCG GACTGCCTAT
    CGACATGGGG AGAAAACTAT GTATAGGCGC AAATCCCAGT GTTGTCCTGG ATTTTATGAA
    AGCAGGGAAA TGTGTGTCCC CCACTGTGCT GATAAATGTG TCCATGGTCG CTGCATTGCT
    CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGTGGAACCA ACTGCTCCAG TGCCTGTGAT
    GGTGATCACT GGGGGCCCCA TTGCAGCAGC CGGTGCCAGT GCAAGAACGG GGCCCTGTGC
    AACCCCATCA CGGGGGCTTG CCACTGCGCT GCAGGTTTCC GGGGCTGGCG TTGTGAGGAC
    CGCTGTGAGC AGGGCACCTA CGGTAATGAC TGTCACCAGA GATGCCAGTG TCAGAATGGA
    GCCACCTGCG ACCACGTCAC AGGGGAATGC CGCTGCCCAC CAGGGTACAC TGGAGCCTTC
    TGTGAGGACC TTTGTCCCCC TGGCAAGCAC GGTCCGCAGT GCGAGCAGAG ATGTCCCTGC
    CAAAATGGAG GAATATGTCA TCACGTCACC GGAGAATGCT CTTGCCCTTC GGGCTGGCTG
    GGCACAGTCT GTGGTCAGCC TTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCACAAGAA
    TGTCAGTGCC ATAATGGAGG GACGTGTGAT GCTGCCACAG GCCAATGTCA TTGCAGTCCA
    GGATACACAG GGGAACGCTG CCAGGACGAG TGTCCCGTTG GCACATACGG AGTTCGCTGT
    GCTGAGACCT GCCAGTGTGT CAATGGAGGG AAGTGTTATC ACGTGAGTGG CTCATGCCTC
    TGTGAAGCAG GCTTTGCTGG TGAGCACTGT GAGGCACGCC TGTGTCCCGA AGGGCTCTAC
    GGCATCAAAT GTGACAAGCG GTGTCCCTGC CACTTGGACA ACACTCATAG CTGTCACCCC
    ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC TGGTCAGGAC TCTACTGTAA CGAAACATGT
    TCTCCTGGAT TCTTTGGGGA AGCTTGCCAA CAGATCTGCA GCTGCCACAA CGGGGCTGAT
    TGTGACAGTG TGACTGGAAA ATGCATCTGT GCCCCAGGAT TCAAAGGAAT TGACTGCTCT
    ATCCCATGCC CATTGGAAAC CTATGGGATA AACTGCTCCT CTCTCTGTGG CTGTAAAAAC
    GATGCTGTCT GTTCACCTGT AGATGGGTCT TGTACTTGCA AGGCAGGCTG GCACGGGGTG
    GACTGCTCCA TCAGCTGTCC CAGTGGCACG TGGGGCTTCA GCTGTAACCT GACCTGCCAG
    TGCCTCCACG GGGGAGCCTG CAACACCCAG GACGGGACCT GCACGTGTGC GCCTGGATGG
    CGCGGGGACA AATGTGAACT CCCCTGCCAG GATGGCACAT ATGGGCTGAA CTGTGCGGAG
    CGCTGTGACT GTAGCCATGC GGATGGCTGC CACTCCACCA CGGGCCACTG CCGCTGCCTC
    CCTGGATGGT CAGGTGTCCA CTGCGACAGC GTGTGTGCTG AGGGGCGCTG GGGGCCCAAC
    TGCTCTCTGC CCTGCTACTG TAAAAATGGG GCTTCATGCT CCCCAGACGA TGGCATCTGT
    GAGTGTGCGC CAGGGTTCCG AGGCACCACC TGTCAGAGAA TCTGCTCCCC TGGTTTTTAT
    GGGCATCGCT GCAGCCAGAC ATGCCCGCAG TGCGTGCATA GCAGTGGGCC CTGCCACCAC
    ATCACAGGCC TGTGTGACTG CTTGCCTGGC TTCACAGGTG CCCTCTGCAA TGAAGTGTGT
    CCCAGTGGCA GATTTGGGAA AAACTGTGCT GGAGTTTGTA CCTGTACAAA CAATGGAACC
    TGTAACCCCA TTGACCGATC TTGTCAGTGT TATCCAGGGT GGATTGGCAG TGACTGCTCG
    CAACCTTGTC CTCCTGCGCA TTGGGGTCCA AACTGCATCC ACACGTGCAA CTGCCACAAT
    GGGGCGTTCT GCAGTGCATA CGACGGGGAA TGTAAATGCA CTCCTGGCTG GACCGGACTC
    TACTGCACTC AGAGATGTCC CCTGGGTTTT TACGGAAAGG ACTGTGCGTT GATATGTCAG
    TGTCAGAATG GAGCTGACTG TGACCACATC TCTGGGCAGT GTACATGCCG CACTGGGTTC
    ATGGGGAAGC ACTGTGAACA GAAATGCCCT GCGGGAACAT ATGGCTATGG CTGTCGCCAG
    ATATGTGACT GTCTGAACAA CTCCACCTGT GACCATATCA CTGGGACTTG TTACTGTAGC
    CCAGGCTGGA AGGGGGCACG GTGTGATCAA GCTGGAGTCA TTATCGTTGG AAATCTGAAC
    AGCTTAAGCA GGACCAGTAC AGCTCTCCCT GCCAATTCCT ACCAGATCGG AGCCATCGTG
    GGCATCATCA TTCTTGTACT GGTTGTTCTC TTCCTCCTGG CGTTGTTCAT TATTTACAGA
    CAGAAGCAAA AGGGAAAGGA ATCAAGCATG CCTGCAGTTA CCTACACCCC TGCCCTGAGG
    GTCATGAATG CAGACTATAC CATTTCAGAA ACCCTTCCCC ACAGCAACAG TGGAAGTGCT
    AACAGCCATT ACTTCACTAA CCCCAGTTAC CACACACTTA CCCAGTGCGC CACATCCCCA
    CATGTCAACA ACAGGGACAG GATGACCATC GCAAAGTCAA AGAACAATCA GCTGTTTGTG
    AATCTGAAAA ATGTGAATCC TGGGAAGAGA GGCCCAGTGG CAGACTGCAC GGGAACATTG
    CCAGCAGACT GGAAACATGG CGGCTACCTT AATGAGCTTG GAGCTTTTGG ACTTGATAGA
    AGTTATATGG GAAAATCCTT GAAAGATTTG GGAAAGAATT CTGAATATAA TTCAAGTAAC
    TGCTCCTTAA GCAGTTCTGA AAACCCATAT GCCACTATTA AAGACCCGCC TGTACTTATC
    CCCAAAAACT CAGAGTGTGG CTATGTGGAA ATGAAGTCAC CAGCACGGAG AGATTCCCCC
    TATGCAGAGA TCAATAACTC AACTTCAGCC AACAAGAATG TCTATGAAGT TGAGCTGTAC
    CGAGGATTGT GCAGTTTATC ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005626535.1
    CDS818..4081
    Translation

    Target ORF information:

    RefSeq Version XM_005626478.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris multiple EGF like domains 10 (MEGF10), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005626478.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGTTGCTT CTTTGAACTC ATGCCTGAGC TTTATCTGTT TCTCATTATG CCTCTGGAGC 
    AGGACAGCAT CAGCACTGAA TCTTGAAGAC CCTAATGTGT GTAGCCACTG GGAAAGCTAC
    TCAGTGACTG TGCAAGAGTC ATACCCACAT CCTTTCGATC AAATCTACTA CACCAGCTGC
    ACTGATATTC TGAACTGGTT TAAATGTACA CGGCACAGAA TCAGTTATCG GACTGCCTAT
    CGACATGGGG AGAAAACTAT GTATAGGCGC AAATCCCAGT GTTGTCCTGG ATTTTATGAA
    AGCAGGGAAA TGTGTGTCCC CCACTGTGCT GATAAATGTG TCCATGGTCG CTGCATTGCT
    CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGTGGAACCA ACTGCTCCAG TGCCTGTGAT
    GGTGATCACT GGGGGCCCCA TTGCAGCAGC CGGTGCCAGT GCAAGAACGG GGCCCTGTGC
    AACCCCATCA CGGGGGCTTG CCACTGCGCT GCAGGTTTCC GGGGCTGGCG TTGTGAGGAC
    CGCTGTGAGC AGGGCACCTA CGGTAATGAC TGTCACCAGA GATGCCAGTG TCAGAATGGA
    GCCACCTGCG ACCACGTCAC AGGGGAATGC CGCTGCCCAC CAGGGTACAC TGGAGCCTTC
    TGTGAGGACC TTTGTCCCCC TGGCAAGCAC GGTCCGCAGT GCGAGCAGAG ATGTCCCTGC
    CAAAATGGAG GAATATGTCA TCACGTCACC GGAGAATGCT CTTGCCCTTC GGGCTGGCTG
    GGCACAGTCT GTGGTCAGCC TTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCACAAGAA
    TGTCAGTGCC ATAATGGAGG GACGTGTGAT GCTGCCACAG GCCAATGTCA TTGCAGTCCA
    GGATACACAG GGGAACGCTG CCAGGACGAG TGTCCCGTTG GCACATACGG AGTTCGCTGT
    GCTGAGACCT GCCAGTGTGT CAATGGAGGG AAGTGTTATC ACGTGAGTGG CTCATGCCTC
    TGTGAAGCAG GCTTTGCTGG TGAGCACTGT GAGGCACGCC TGTGTCCCGA AGGGCTCTAC
    GGCATCAAAT GTGACAAGCG GTGTCCCTGC CACTTGGACA ACACTCATAG CTGTCACCCC
    ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC TGGTCAGGAC TCTACTGTAA CGAAACATGT
    TCTCCTGGAT TCTTTGGGGA AGCTTGCCAA CAGATCTGCA GCTGCCACAA CGGGGCTGAT
    TGTGACAGTG TGACTGGAAA ATGCATCTGT GCCCCAGGAT TCAAAGGAAT TGACTGCTCT
    ATCCCATGCC CATTGGAAAC CTATGGGATA AACTGCTCCT CTCTCTGTGG CTGTAAAAAC
    GATGCTGTCT GTTCACCTGT AGATGGGTCT TGTACTTGCA AGGCAGGCTG GCACGGGGTG
    GACTGCTCCA TCAGCTGTCC CAGTGGCACG TGGGGCTTCA GCTGTAACCT GACCTGCCAG
    TGCCTCCACG GGGGAGCCTG CAACACCCAG GACGGGACCT GCACGTGTGC GCCTGGATGG
    CGCGGGGACA AATGTGAACT CCCCTGCCAG GATGGCACAT ATGGGCTGAA CTGTGCGGAG
    CGCTGTGACT GTAGCCATGC GGATGGCTGC CACTCCACCA CGGGCCACTG CCGCTGCCTC
    CCTGGATGGT CAGGTGTCCA CTGCGACAGC GTGTGTGCTG AGGGGCGCTG GGGGCCCAAC
    TGCTCTCTGC CCTGCTACTG TAAAAATGGG GCTTCATGCT CCCCAGACGA TGGCATCTGT
    GAGTGTGCGC CAGGGTTCCG AGGCACCACC TGTCAGAGAA TCTGCTCCCC TGGTTTTTAT
    GGGCATCGCT GCAGCCAGAC ATGCCCGCAG TGCGTGCATA GCAGTGGGCC CTGCCACCAC
    ATCACAGGCC TGTGTGACTG CTTGCCTGGC TTCACAGGTG CCCTCTGCAA TGAAGTGTGT
    CCCAGTGGCA GATTTGGGAA AAACTGTGCT GGAGTTTGTA CCTGTACAAA CAATGGAACC
    TGTAACCCCA TTGACCGATC TTGTCAGTGT TATCCAGGGT GGATTGGCAG TGACTGCTCG
    CAACCTTGTC CTCCTGCGCA TTGGGGTCCA AACTGCATCC ACACGTGCAA CTGCCACAAT
    GGGGCGTTCT GCAGTGCATA CGACGGGGAA TGTAAATGCA CTCCTGGCTG GACCGGACTC
    TACTGCACTC AGAGATGTCC CCTGGGTTTT TACGGAAAGG ACTGTGCGTT GATATGTCAG
    TGTCAGAATG GAGCTGACTG TGACCACATC TCTGGGCAGT GTACATGCCG CACTGGGTTC
    ATGGGGAAGC ACTGTGAACA GAAATGCCCT GCGGGAACAT ATGGCTATGG CTGTCGCCAG
    ATATGTGACT GTCTGAACAA CTCCACCTGT GACCATATCA CTGGGACTTG TTACTGTAGC
    CCAGGCTGGA AGGGGGCACG GTGTGATCAA GCTGGAGTCA TTATCGTTGG AAATCTGAAC
    AGCTTAAGCA GGACCAGTAC AGCTCTCCCT GCCAATTCCT ACCAGATCGG AGCCATCGTG
    GGCATCATCA TTCTTGTACT GGTTGTTCTC TTCCTCCTGG CGTTGTTCAT TATTTACAGA
    CAGAAGCAAA AGGGAAAGGA ATCAAGCATG CCTGCAGTTA CCTACACCCC TGCCCTGAGG
    GTCATGAATG CAGACTATAC CATTTCAGAA ACCCTTCCCC ACAGCAACAG TGGAAGTGCT
    AACAGCCATT ACTTCACTAA CCCCAGTTAC CACACACTTA CCCAGTGCGC CACATCCCCA
    CATGTCAACA ACAGGGACAG GATGACCATC GCAAAGTCAA AGAACAATCA GCTGTTTGTG
    AATCTGAAAA ATGTGAATCC TGGGAAGAGA GGCCCAGTGG CAGACTGCAC GGGAACATTG
    CCAGCAGACT GGAAACATGG CGGCTACCTT AATGAGCTTG GAGCTTTTGG ACTTGATAGA
    AGTTATATGG GAAAATCCTT GAAAGATTTG GGAAAGAATT CTGAATATAA TTCAAGTAAC
    TGCTCCTTAA GCAGTTCTGA AAACCCATAT GCCACTATTA AAGACCCGCC TGTACTTATC
    CCCAAAAACT CAGAGTGTGG CTATGTGGAA ATGAAGTCAC CAGCACGGAG AGATTCCCCC
    TATGCAGAGA TCAATAACTC AACTTCAGCC AACAAGAATG TCTATGAAGT TGAGCTGTAC
    CGAGGATTGT GCAGTTTATC ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe14213
    Clone ID Related Accession (Same CDS sequence) XM_005626478.3 , XM_005626478.2
    Accession Version XM_005626478.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3264bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product multiple epidermal growth factor-like domains protein 10 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005626478.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGTTGCTT CTTTGAACTC ATGCCTGAGC TTTATCTGTT TCTCATTATG CCTCTGGAGC 
    AGGACAGCAT CAGCACTGAA TCTTGAAGAC CCTAATGTGT GTAGCCACTG GGAAAGCTAC
    TCAGTGACTG TGCAAGAGTC ATACCCACAT CCTTTCGATC AAATCTACTA CACCAGCTGC
    ACTGATATTC TGAACTGGTT TAAATGTACA CGGCACAGAA TCAGTTATCG GACTGCCTAT
    CGACATGGGG AGAAAACTAT GTATAGGCGC AAATCCCAGT GTTGTCCTGG ATTTTATGAA
    AGCAGGGAAA TGTGTGTCCC CCACTGTGCT GATAAATGTG TCCATGGTCG CTGCATTGCT
    CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGTGGAACCA ACTGCTCCAG TGCCTGTGAT
    GGTGATCACT GGGGGCCCCA TTGCAGCAGC CGGTGCCAGT GCAAGAACGG GGCCCTGTGC
    AACCCCATCA CGGGGGCTTG CCACTGCGCT GCAGGTTTCC GGGGCTGGCG TTGTGAGGAC
    CGCTGTGAGC AGGGCACCTA CGGTAATGAC TGTCACCAGA GATGCCAGTG TCAGAATGGA
    GCCACCTGCG ACCACGTCAC AGGGGAATGC CGCTGCCCAC CAGGGTACAC TGGAGCCTTC
    TGTGAGGACC TTTGTCCCCC TGGCAAGCAC GGTCCGCAGT GCGAGCAGAG ATGTCCCTGC
    CAAAATGGAG GAATATGTCA TCACGTCACC GGAGAATGCT CTTGCCCTTC GGGCTGGCTG
    GGCACAGTCT GTGGTCAGCC TTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCACAAGAA
    TGTCAGTGCC ATAATGGAGG GACGTGTGAT GCTGCCACAG GCCAATGTCA TTGCAGTCCA
    GGATACACAG GGGAACGCTG CCAGGACGAG TGTCCCGTTG GCACATACGG AGTTCGCTGT
    GCTGAGACCT GCCAGTGTGT CAATGGAGGG AAGTGTTATC ACGTGAGTGG CTCATGCCTC
    TGTGAAGCAG GCTTTGCTGG TGAGCACTGT GAGGCACGCC TGTGTCCCGA AGGGCTCTAC
    GGCATCAAAT GTGACAAGCG GTGTCCCTGC CACTTGGACA ACACTCATAG CTGTCACCCC
    ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC TGGTCAGGAC TCTACTGTAA CGAAACATGT
    TCTCCTGGAT TCTTTGGGGA AGCTTGCCAA CAGATCTGCA GCTGCCACAA CGGGGCTGAT
    TGTGACAGTG TGACTGGAAA ATGCATCTGT GCCCCAGGAT TCAAAGGAAT TGACTGCTCT
    ATCCCATGCC CATTGGAAAC CTATGGGATA AACTGCTCCT CTCTCTGTGG CTGTAAAAAC
    GATGCTGTCT GTTCACCTGT AGATGGGTCT TGTACTTGCA AGGCAGGCTG GCACGGGGTG
    GACTGCTCCA TCAGCTGTCC CAGTGGCACG TGGGGCTTCA GCTGTAACCT GACCTGCCAG
    TGCCTCCACG GGGGAGCCTG CAACACCCAG GACGGGACCT GCACGTGTGC GCCTGGATGG
    CGCGGGGACA AATGTGAACT CCCCTGCCAG GATGGCACAT ATGGGCTGAA CTGTGCGGAG
    CGCTGTGACT GTAGCCATGC GGATGGCTGC CACTCCACCA CGGGCCACTG CCGCTGCCTC
    CCTGGATGGT CAGGTGTCCA CTGCGACAGC GTGTGTGCTG AGGGGCGCTG GGGGCCCAAC
    TGCTCTCTGC CCTGCTACTG TAAAAATGGG GCTTCATGCT CCCCAGACGA TGGCATCTGT
    GAGTGTGCGC CAGGGTTCCG AGGCACCACC TGTCAGAGAA TCTGCTCCCC TGGTTTTTAT
    GGGCATCGCT GCAGCCAGAC ATGCCCGCAG TGCGTGCATA GCAGTGGGCC CTGCCACCAC
    ATCACAGGCC TGTGTGACTG CTTGCCTGGC TTCACAGGTG CCCTCTGCAA TGAAGTGTGT
    CCCAGTGGCA GATTTGGGAA AAACTGTGCT GGAGTTTGTA CCTGTACAAA CAATGGAACC
    TGTAACCCCA TTGACCGATC TTGTCAGTGT TATCCAGGGT GGATTGGCAG TGACTGCTCG
    CAACCTTGTC CTCCTGCGCA TTGGGGTCCA AACTGCATCC ACACGTGCAA CTGCCACAAT
    GGGGCGTTCT GCAGTGCATA CGACGGGGAA TGTAAATGCA CTCCTGGCTG GACCGGACTC
    TACTGCACTC AGAGATGTCC CCTGGGTTTT TACGGAAAGG ACTGTGCGTT GATATGTCAG
    TGTCAGAATG GAGCTGACTG TGACCACATC TCTGGGCAGT GTACATGCCG CACTGGGTTC
    ATGGGGAAGC ACTGTGAACA GAAATGCCCT GCGGGAACAT ATGGCTATGG CTGTCGCCAG
    ATATGTGACT GTCTGAACAA CTCCACCTGT GACCATATCA CTGGGACTTG TTACTGTAGC
    CCAGGCTGGA AGGGGGCACG GTGTGATCAA GCTGGAGTCA TTATCGTTGG AAATCTGAAC
    AGCTTAAGCA GGACCAGTAC AGCTCTCCCT GCCAATTCCT ACCAGATCGG AGCCATCGTG
    GGCATCATCA TTCTTGTACT GGTTGTTCTC TTCCTCCTGG CGTTGTTCAT TATTTACAGA
    CAGAAGCAAA AGGGAAAGGA ATCAAGCATG CCTGCAGTTA CCTACACCCC TGCCCTGAGG
    GTCATGAATG CAGACTATAC CATTTCAGAA ACCCTTCCCC ACAGCAACAG TGGAAGTGCT
    AACAGCCATT ACTTCACTAA CCCCAGTTAC CACACACTTA CCCAGTGCGC CACATCCCCA
    CATGTCAACA ACAGGGACAG GATGACCATC GCAAAGTCAA AGAACAATCA GCTGTTTGTG
    AATCTGAAAA ATGTGAATCC TGGGAAGAGA GGCCCAGTGG CAGACTGCAC GGGAACATTG
    CCAGCAGACT GGAAACATGG CGGCTACCTT AATGAGCTTG GAGCTTTTGG ACTTGATAGA
    AGTTATATGG GAAAATCCTT GAAAGATTTG GGAAAGAATT CTGAATATAA TTCAAGTAAC
    TGCTCCTTAA GCAGTTCTGA AAACCCATAT GCCACTATTA AAGACCCGCC TGTACTTATC
    CCCAAAAACT CAGAGTGTGG CTATGTGGAA ATGAAGTCAC CAGCACGGAG AGATTCCCCC
    TATGCAGAGA TCAATAACTC AACTTCAGCC AACAAGAATG TCTATGAAGT TGAGCTGTAC
    CGAGGATTGT GCAGTTTATC ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005626535.1
    CDS800..4063
    Translation

    Target ORF information:

    RefSeq Version XM_005626478.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris multiple EGF-like-domains 10 (MEGF10), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005626478.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGTTGCTT CTTTGAACTC ATGCCTGAGC TTTATCTGTT TCTCATTATG CCTCTGGAGC 
    AGGACAGCAT CAGCACTGAA TCTTGAAGAC CCTAATGTGT GTAGCCACTG GGAAAGCTAC
    TCAGTGACTG TGCAAGAGTC ATACCCACAT CCTTTCGATC AAATCTACTA CACCAGCTGC
    ACTGATATTC TGAACTGGTT TAAATGTACA CGGCACAGAA TCAGTTATCG GACTGCCTAT
    CGACATGGGG AGAAAACTAT GTATAGGCGC AAATCCCAGT GTTGTCCTGG ATTTTATGAA
    AGCAGGGAAA TGTGTGTCCC CCACTGTGCT GATAAATGTG TCCATGGTCG CTGCATTGCT
    CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGTGGAACCA ACTGCTCCAG TGCCTGTGAT
    GGTGATCACT GGGGGCCCCA TTGCAGCAGC CGGTGCCAGT GCAAGAACGG GGCCCTGTGC
    AACCCCATCA CGGGGGCTTG CCACTGCGCT GCAGGTTTCC GGGGCTGGCG TTGTGAGGAC
    CGCTGTGAGC AGGGCACCTA CGGTAATGAC TGTCACCAGA GATGCCAGTG TCAGAATGGA
    GCCACCTGCG ACCACGTCAC AGGGGAATGC CGCTGCCCAC CAGGGTACAC TGGAGCCTTC
    TGTGAGGACC TTTGTCCCCC TGGCAAGCAC GGTCCGCAGT GCGAGCAGAG ATGTCCCTGC
    CAAAATGGAG GAATATGTCA TCACGTCACC GGAGAATGCT CTTGCCCTTC GGGCTGGCTG
    GGCACAGTCT GTGGTCAGCC TTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCACAAGAA
    TGTCAGTGCC ATAATGGAGG GACGTGTGAT GCTGCCACAG GCCAATGTCA TTGCAGTCCA
    GGATACACAG GGGAACGCTG CCAGGACGAG TGTCCCGTTG GCACATACGG AGTTCGCTGT
    GCTGAGACCT GCCAGTGTGT CAATGGAGGG AAGTGTTATC ACGTGAGTGG CTCATGCCTC
    TGTGAAGCAG GCTTTGCTGG TGAGCACTGT GAGGCACGCC TGTGTCCCGA AGGGCTCTAC
    GGCATCAAAT GTGACAAGCG GTGTCCCTGC CACTTGGACA ACACTCATAG CTGTCACCCC
    ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC TGGTCAGGAC TCTACTGTAA CGAAACATGT
    TCTCCTGGAT TCTTTGGGGA AGCTTGCCAA CAGATCTGCA GCTGCCACAA CGGGGCTGAT
    TGTGACAGTG TGACTGGAAA ATGCATCTGT GCCCCAGGAT TCAAAGGAAT TGACTGCTCT
    ATCCCATGCC CATTGGAAAC CTATGGGATA AACTGCTCCT CTCTCTGTGG CTGTAAAAAC
    GATGCTGTCT GTTCACCTGT AGATGGGTCT TGTACTTGCA AGGCAGGCTG GCACGGGGTG
    GACTGCTCCA TCAGCTGTCC CAGTGGCACG TGGGGCTTCA GCTGTAACCT GACCTGCCAG
    TGCCTCCACG GGGGAGCCTG CAACACCCAG GACGGGACCT GCACGTGTGC GCCTGGATGG
    CGCGGGGACA AATGTGAACT CCCCTGCCAG GATGGCACAT ATGGGCTGAA CTGTGCGGAG
    CGCTGTGACT GTAGCCATGC GGATGGCTGC CACTCCACCA CGGGCCACTG CCGCTGCCTC
    CCTGGATGGT CAGGTGTCCA CTGCGACAGC GTGTGTGCTG AGGGGCGCTG GGGGCCCAAC
    TGCTCTCTGC CCTGCTACTG TAAAAATGGG GCTTCATGCT CCCCAGACGA TGGCATCTGT
    GAGTGTGCGC CAGGGTTCCG AGGCACCACC TGTCAGAGAA TCTGCTCCCC TGGTTTTTAT
    GGGCATCGCT GCAGCCAGAC ATGCCCGCAG TGCGTGCATA GCAGTGGGCC CTGCCACCAC
    ATCACAGGCC TGTGTGACTG CTTGCCTGGC TTCACAGGTG CCCTCTGCAA TGAAGTGTGT
    CCCAGTGGCA GATTTGGGAA AAACTGTGCT GGAGTTTGTA CCTGTACAAA CAATGGAACC
    TGTAACCCCA TTGACCGATC TTGTCAGTGT TATCCAGGGT GGATTGGCAG TGACTGCTCG
    CAACCTTGTC CTCCTGCGCA TTGGGGTCCA AACTGCATCC ACACGTGCAA CTGCCACAAT
    GGGGCGTTCT GCAGTGCATA CGACGGGGAA TGTAAATGCA CTCCTGGCTG GACCGGACTC
    TACTGCACTC AGAGATGTCC CCTGGGTTTT TACGGAAAGG ACTGTGCGTT GATATGTCAG
    TGTCAGAATG GAGCTGACTG TGACCACATC TCTGGGCAGT GTACATGCCG CACTGGGTTC
    ATGGGGAAGC ACTGTGAACA GAAATGCCCT GCGGGAACAT ATGGCTATGG CTGTCGCCAG
    ATATGTGACT GTCTGAACAA CTCCACCTGT GACCATATCA CTGGGACTTG TTACTGTAGC
    CCAGGCTGGA AGGGGGCACG GTGTGATCAA GCTGGAGTCA TTATCGTTGG AAATCTGAAC
    AGCTTAAGCA GGACCAGTAC AGCTCTCCCT GCCAATTCCT ACCAGATCGG AGCCATCGTG
    GGCATCATCA TTCTTGTACT GGTTGTTCTC TTCCTCCTGG CGTTGTTCAT TATTTACAGA
    CAGAAGCAAA AGGGAAAGGA ATCAAGCATG CCTGCAGTTA CCTACACCCC TGCCCTGAGG
    GTCATGAATG CAGACTATAC CATTTCAGAA ACCCTTCCCC ACAGCAACAG TGGAAGTGCT
    AACAGCCATT ACTTCACTAA CCCCAGTTAC CACACACTTA CCCAGTGCGC CACATCCCCA
    CATGTCAACA ACAGGGACAG GATGACCATC GCAAAGTCAA AGAACAATCA GCTGTTTGTG
    AATCTGAAAA ATGTGAATCC TGGGAAGAGA GGCCCAGTGG CAGACTGCAC GGGAACATTG
    CCAGCAGACT GGAAACATGG CGGCTACCTT AATGAGCTTG GAGCTTTTGG ACTTGATAGA
    AGTTATATGG GAAAATCCTT GAAAGATTTG GGAAAGAATT CTGAATATAA TTCAAGTAAC
    TGCTCCTTAA GCAGTTCTGA AAACCCATAT GCCACTATTA AAGACCCGCC TGTACTTATC
    CCCAAAAACT CAGAGTGTGG CTATGTGGAA ATGAAGTCAC CAGCACGGAG AGATTCCCCC
    TATGCAGAGA TCAATAACTC AACTTCAGCC AACAAGAATG TCTATGAAGT TGAGCTGTAC
    CGAGGATTGT GCAGTTTATC ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe291935
    Clone ID Related Accession (Same CDS sequence) XM_022425235.1
    Accession Version XM_022425235.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3444bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product multiple epidermal growth factor-like domains protein 10 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCCAGAA GAGGTTTTTC TTCCGAAAAA ATGGTTGCTT CTTTGAACTC ATGCCTGAGC 
    TTTATCTGTT TCTCATTATG CCTCTGGAGC AGGACAGCAT CAGCACTGAA TCTTGAAGAC
    CCTAATGTGT GTAGCCACTG GGAAAGCTAC TCAGTGACTG TGCAAGAGTC ATACCCACAT
    CCTTTCGATC AAATCTACTA CACCAGCTGC ACTGATATTC TGAACTGGTT TAAATGTACA
    CGGCACAGAA TCAGTTATCG GACTGCCTAT CGACATGGGG AGAAAACTAT GTATAGGCGC
    AAATCCCAGT GTTGTCCTGG ATTTTATGAA AGCAGGGAAA TGTGTGTCCC CCACTGTGCT
    GATAAATGTG TCCATGGTCG CTGCATTGCT CCAAACACCT GTCAGTGTGA GCCTGGCTGG
    GGTGGAACCA ACTGCTCCAG TGCCTGTGAT GGTGATCACT GGGGGCCCCA TTGCAGCAGC
    CGGTGCCAGT GCAAGAACGG GGCCCTGTGC AACCCCATCA CGGGGGCTTG CCACTGCGCT
    GCAGGTTTCC GGGGCTGGCG TTGTGAGGAC CGCTGTGAGC AGGGCACCTA CGGTAATGAC
    TGTCACCAGA GATGCCAGTG TCAGAATGGA GCCACCTGCG ACCACGTCAC AGGGGAATGC
    CGCTGCCCAC CAGGGTACAC TGGAGCCTTC TGTGAGGACC TTTGTCCCCC TGGCAAGCAC
    GGTCCGCAGT GCGAGCAGAG ATGTCCCTGC CAAAATGGAG GAATATGTCA TCACGTCACC
    GGAGAATGCT CTTGCCCTTC GGGCTGGCTG GGCACAGTCT GTGGTCAGCC TTGCCCCGAG
    GGTCGCTTTG GAAAGAACTG TTCACAAGAA TGTCAGTGCC ATAATGGAGG GACGTGTGAT
    GCTGCCACAG GCCAATGTCA TTGCAGTCCA GGATACACAG GGGAACGCTG CCAGGACGAG
    TGTCCCGTTG GCACATACGG AGTTCGCTGT GCTGAGACCT GCCAGTGTGT CAATGGAGGG
    AAGTGTTATC ACGTGAGTGG CTCATGCCTC TGTGAAGCAG GCTTTGCTGG TGAGCACTGT
    GAGGCACGCC TGTGTCCCGA AGGGCTCTAC GGCATCAAAT GTGACAAGCG GTGTCCCTGC
    CACTTGGACA ACACTCATAG CTGTCACCCC ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC
    TGGTCAGGAC TCTACTGTAA CGAAACATGT TCTCCTGGAT TCTTTGGGGA AGCTTGCCAA
    CAGATCTGCA GCTGCCACAA CGGGGCTGAT TGTGACAGTG TGACTGGAAA ATGCATCTGT
    GCCCCAGGAT TCAAAGGAAT TGACTGCTCT ATCCCATGCC CATTGGAAAC CTATGGGATA
    AACTGCTCCT CTCTCTGTGG CTGTAAAAAC GATGCTGTCT GTTCACCTGT AGATGGGTCT
    TGTACTTGCA AGGCAGGCTG GCACGGGGTG GACTGCTCCA TCAGCTGTCC CAGTGGCACG
    TGGGGCTTCA GCTGTAACCT GACCTGCCAG TGCCTCCACG GGGGAGCCTG CAACACCCAG
    GACGGGACCT GCACGTGTGC GCCTGGATGG CGCGGGGACA AATGTGAACT CCCCTGCCAG
    GATGGCACAT ATGGGCTGAA CTGTGCGGAG CGCTGTGACT GTAGCCATGC GGATGGCTGC
    CACTCCACCA CGGGCCACTG CCGCTGCCTC CCTGGATGGT CAGGTGTCCA CTGCGACAGC
    GTGTGTGCTG AGGGGCGCTG GGGGCCCAAC TGCTCTCTGC CCTGCTACTG TAAAAATGGG
    GCTTCATGCT CCCCAGACGA TGGCATCTGT GAGTGTGCGC CAGGGTTCCG AGGCACCACC
    TGTCAGAGAA TCTGCTCCCC TGGTTTTTAT GGGCATCGCT GCAGCCAGAC ATGCCCGCAG
    TGCGTGCATA GCAGTGGGCC CTGCCACCAC ATCACAGGCC TGTGTGACTG CTTGCCTGGC
    TTCACAGGTG CCCTCTGCAA TGAAGTGTGT CCCAGTGGCA GATTTGGGAA AAACTGTGCT
    GGAGTTTGTA CCTGTACAAA CAATGGAACC TGTAACCCCA TTGACCGATC TTGTCAGTGT
    TATCCAGGGT GGATTGGCAG TGACTGCTCG CAACCTTGTC CTCCTGCGCA TTGGGGTCCA
    AACTGCATCC ACACGTGCAA CTGCCACAAT GGGGCGTTCT GCAGTGCATA CGACGGGGAA
    TGTAAATGCA CTCCTGGCTG GACCGGACTC TACTGCACTC AGAGATGTCC CCTGGGTTTT
    TACGGAAAGG ACTGTGCGTT GATATGTCAG TGTCAGAATG GAGCTGACTG TGACCACATC
    TCTGGGCAGT GTACATGCCG CACTGGGTTC ATGGGGAAGC ACTGTGAACA GAAATGCCCT
    GCGGGAACAT ATGGCTATGG CTGTCGCCAG ATATGTGACT GTCTGAACAA CTCCACCTGT
    GACCATATCA CTGGGACTTG TTACTGTAGC CCAGGCTGGA AGGGGGCACG GTGTGATCAA
    GCTGGAGTCA TTATCGTTGG AAATCTGAAC AGCTTAAGCA GGACCAGTAC AGCTCTCCCT
    GCCAATTCCT ACCAGATCGG AGCCATCGTG GGCATCATCA TTCTTGTACT GGTTGTTCTC
    TTCCTCCTGG CGTTGTTCAT TATTTACAGA CAGAAGCAAA AGGGAAAGGA ATCAAGCATG
    CCTGCAGTTA CCTACACCCC TGCCCTGAGG GTCATGAATG CAGACTATAC CATTTCAGAA
    ACCCTTCCCC ACAGCAACAG TGGAAGTGCT AACAGCCATT ACTTCACTAA CCCCAGTTAC
    CACACACTTA CCCAGTGCGC CACATCCCCA CATGTCAACA ACAGGGACAG GATGACCATC
    GCAAAGTCAA AGAACAATCA GCTGTTTGTG AATCTGAAAA ATGTGAATCC TGGGAAGAGA
    GGCCCAGTGG CAGACTGCAC GGGAACATTG CCAGCAGACT GGAAACATGG CGGCTACCTT
    AATGAGCTTG GAGCTTTTGG ACTTGATAGA AGTTATATGG GAAAATCCTT GAAAGATTTG
    GGAAAGAATT CTGAATATAA TTCAAGTAAC TGCTCCTTAA GCAGTTCTGA AAACCCATAT
    GCCACTATTA AAGACCCGCC TGTACTTATC CCCAAAAACT CAGAGTGTGG CTATGTGGAA
    ATGAAGTCAC CAGCACGGAG AGATTCCCCC TATGCAGAGA TCAATAACTC AACTTCAGCC
    AACAAGAATG TCTATGAAGT TGAACCTACA GTGAGTGTTG TCCAAGGAAT ATTCAGCAAT
    AATGGGCATC TCACCCAGGA TCCATACGAC CTCCCAAAGA ACAGTCACAT CCCTTGCCAT
    TATGACCTGC TGCCAGTCCG AGACAGTTCA TCCTCCCCCA AGCGAGAGGA TGGTGGCAGT
    GGCAGCAGCA GCAGCAGTGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022280943.1
    CDS554..3997
    Translation

    Target ORF information:

    RefSeq Version XM_022425235.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris multiple EGF like domains 10 (MEGF10), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022425235.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCCAGAA GAGGTTTTTC TTCCGAAAAA ATGGTTGCTT CTTTGAACTC ATGCCTGAGC 
    TTTATCTGTT TCTCATTATG CCTCTGGAGC AGGACAGCAT CAGCACTGAA TCTTGAAGAC
    CCTAATGTGT GTAGCCACTG GGAAAGCTAC TCAGTGACTG TGCAAGAGTC ATACCCACAT
    CCTTTCGATC AAATCTACTA CACCAGCTGC ACTGATATTC TGAACTGGTT TAAATGTACA
    CGGCACAGAA TCAGTTATCG GACTGCCTAT CGACATGGGG AGAAAACTAT GTATAGGCGC
    AAATCCCAGT GTTGTCCTGG ATTTTATGAA AGCAGGGAAA TGTGTGTCCC CCACTGTGCT
    GATAAATGTG TCCATGGTCG CTGCATTGCT CCAAACACCT GTCAGTGTGA GCCTGGCTGG
    GGTGGAACCA ACTGCTCCAG TGCCTGTGAT GGTGATCACT GGGGGCCCCA TTGCAGCAGC
    CGGTGCCAGT GCAAGAACGG GGCCCTGTGC AACCCCATCA CGGGGGCTTG CCACTGCGCT
    GCAGGTTTCC GGGGCTGGCG TTGTGAGGAC CGCTGTGAGC AGGGCACCTA CGGTAATGAC
    TGTCACCAGA GATGCCAGTG TCAGAATGGA GCCACCTGCG ACCACGTCAC AGGGGAATGC
    CGCTGCCCAC CAGGGTACAC TGGAGCCTTC TGTGAGGACC TTTGTCCCCC TGGCAAGCAC
    GGTCCGCAGT GCGAGCAGAG ATGTCCCTGC CAAAATGGAG GAATATGTCA TCACGTCACC
    GGAGAATGCT CTTGCCCTTC GGGCTGGCTG GGCACAGTCT GTGGTCAGCC TTGCCCCGAG
    GGTCGCTTTG GAAAGAACTG TTCACAAGAA TGTCAGTGCC ATAATGGAGG GACGTGTGAT
    GCTGCCACAG GCCAATGTCA TTGCAGTCCA GGATACACAG GGGAACGCTG CCAGGACGAG
    TGTCCCGTTG GCACATACGG AGTTCGCTGT GCTGAGACCT GCCAGTGTGT CAATGGAGGG
    AAGTGTTATC ACGTGAGTGG CTCATGCCTC TGTGAAGCAG GCTTTGCTGG TGAGCACTGT
    GAGGCACGCC TGTGTCCCGA AGGGCTCTAC GGCATCAAAT GTGACAAGCG GTGTCCCTGC
    CACTTGGACA ACACTCATAG CTGTCACCCC ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC
    TGGTCAGGAC TCTACTGTAA CGAAACATGT TCTCCTGGAT TCTTTGGGGA AGCTTGCCAA
    CAGATCTGCA GCTGCCACAA CGGGGCTGAT TGTGACAGTG TGACTGGAAA ATGCATCTGT
    GCCCCAGGAT TCAAAGGAAT TGACTGCTCT ATCCCATGCC CATTGGAAAC CTATGGGATA
    AACTGCTCCT CTCTCTGTGG CTGTAAAAAC GATGCTGTCT GTTCACCTGT AGATGGGTCT
    TGTACTTGCA AGGCAGGCTG GCACGGGGTG GACTGCTCCA TCAGCTGTCC CAGTGGCACG
    TGGGGCTTCA GCTGTAACCT GACCTGCCAG TGCCTCCACG GGGGAGCCTG CAACACCCAG
    GACGGGACCT GCACGTGTGC GCCTGGATGG CGCGGGGACA AATGTGAACT CCCCTGCCAG
    GATGGCACAT ATGGGCTGAA CTGTGCGGAG CGCTGTGACT GTAGCCATGC GGATGGCTGC
    CACTCCACCA CGGGCCACTG CCGCTGCCTC CCTGGATGGT CAGGTGTCCA CTGCGACAGC
    GTGTGTGCTG AGGGGCGCTG GGGGCCCAAC TGCTCTCTGC CCTGCTACTG TAAAAATGGG
    GCTTCATGCT CCCCAGACGA TGGCATCTGT GAGTGTGCGC CAGGGTTCCG AGGCACCACC
    TGTCAGAGAA TCTGCTCCCC TGGTTTTTAT GGGCATCGCT GCAGCCAGAC ATGCCCGCAG
    TGCGTGCATA GCAGTGGGCC CTGCCACCAC ATCACAGGCC TGTGTGACTG CTTGCCTGGC
    TTCACAGGTG CCCTCTGCAA TGAAGTGTGT CCCAGTGGCA GATTTGGGAA AAACTGTGCT
    GGAGTTTGTA CCTGTACAAA CAATGGAACC TGTAACCCCA TTGACCGATC TTGTCAGTGT
    TATCCAGGGT GGATTGGCAG TGACTGCTCG CAACCTTGTC CTCCTGCGCA TTGGGGTCCA
    AACTGCATCC ACACGTGCAA CTGCCACAAT GGGGCGTTCT GCAGTGCATA CGACGGGGAA
    TGTAAATGCA CTCCTGGCTG GACCGGACTC TACTGCACTC AGAGATGTCC CCTGGGTTTT
    TACGGAAAGG ACTGTGCGTT GATATGTCAG TGTCAGAATG GAGCTGACTG TGACCACATC
    TCTGGGCAGT GTACATGCCG CACTGGGTTC ATGGGGAAGC ACTGTGAACA GAAATGCCCT
    GCGGGAACAT ATGGCTATGG CTGTCGCCAG ATATGTGACT GTCTGAACAA CTCCACCTGT
    GACCATATCA CTGGGACTTG TTACTGTAGC CCAGGCTGGA AGGGGGCACG GTGTGATCAA
    GCTGGAGTCA TTATCGTTGG AAATCTGAAC AGCTTAAGCA GGACCAGTAC AGCTCTCCCT
    GCCAATTCCT ACCAGATCGG AGCCATCGTG GGCATCATCA TTCTTGTACT GGTTGTTCTC
    TTCCTCCTGG CGTTGTTCAT TATTTACAGA CAGAAGCAAA AGGGAAAGGA ATCAAGCATG
    CCTGCAGTTA CCTACACCCC TGCCCTGAGG GTCATGAATG CAGACTATAC CATTTCAGAA
    ACCCTTCCCC ACAGCAACAG TGGAAGTGCT AACAGCCATT ACTTCACTAA CCCCAGTTAC
    CACACACTTA CCCAGTGCGC CACATCCCCA CATGTCAACA ACAGGGACAG GATGACCATC
    GCAAAGTCAA AGAACAATCA GCTGTTTGTG AATCTGAAAA ATGTGAATCC TGGGAAGAGA
    GGCCCAGTGG CAGACTGCAC GGGAACATTG CCAGCAGACT GGAAACATGG CGGCTACCTT
    AATGAGCTTG GAGCTTTTGG ACTTGATAGA AGTTATATGG GAAAATCCTT GAAAGATTTG
    GGAAAGAATT CTGAATATAA TTCAAGTAAC TGCTCCTTAA GCAGTTCTGA AAACCCATAT
    GCCACTATTA AAGACCCGCC TGTACTTATC CCCAAAAACT CAGAGTGTGG CTATGTGGAA
    ATGAAGTCAC CAGCACGGAG AGATTCCCCC TATGCAGAGA TCAATAACTC AACTTCAGCC
    AACAAGAATG TCTATGAAGT TGAACCTACA GTGAGTGTTG TCCAAGGAAT ATTCAGCAAT
    AATGGGCATC TCACCCAGGA TCCATACGAC CTCCCAAAGA ACAGTCACAT CCCTTGCCAT
    TATGACCTGC TGCCAGTCCG AGACAGTTCA TCCTCCCCCA AGCGAGAGGA TGGTGGCAGT
    GGCAGCAGCA GCAGCAGTGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.