P2RX7 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	P2RX7
Entrez Gene ID	448778
Full Name	purinergic receptor P2X 7
Synonyms	P2X7
General protein information	Preferred Names purinergic receptor P2X 7 Names P2X purinoceptor 7 P2X7 purinoceptor purinergic receptor P2X, ligand-gated ion channel, 7
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001113456.1	NP_001106927.1	P2X purinoceptor 7
XM_014107713.1	XP_013963188.1	P2X purinoceptor 7 isoform X1
XM_014107714.1	XP_013963189.1	P2X purinoceptor 7 isoform X2
XM_014107713.2	XP_013963188.1	P2X purinoceptor 7 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	P2RX7	NP_001193445.1
Mus musculus (house mouse)	P2rx7	NP_035157.2
Xenopus tropicalis (tropical clawed frog)	LOC100490828	XP_002937746.1
Homo sapiens (human)	P2RX7	NP_002553.3
Danio rerio (zebrafish)	p2rx7	NP_945335.1
Pan troglodytes (chimpanzee)	P2RX7	XP_509436.2
	P2RX7	XP_001092531.1
Xenopus tropicalis (tropical clawed frog)	LOC100490662	XP_002937745.1
Canis lupus familiaris (dog)	P2RX7	NP_001106927.1
Gallus gallus (chicken)	P2RX7	XP_001235163.3

Gene Ontology
Bibliography

Related articles in PubMed

Utilizing the Dog Genome in the Search for Novel Candidate Genes Involved in Glioma Development-Genome Wide Association Mapping followed by Targeted Massive Parallel Sequencing Identifies a Strongly Associated Locus.
Truv? K, Dickinson P, Xiong A, York D, Jayashankar K, Pielberg G, Koltookian M, Mur?n E, Fuxelius HH, Weishaupt H, Swartling FJ, Andersson G, Hedhammar ?, Bongcam-Rudloff E, Forsberg-Nilsson K, Bannasch D, Lindblad-Toh K
PLoS genetics12(5)e1006000(2016 May)

R270C polymorphism leads to loss of function of the canine P2X7 receptor.
Spildrejorde M, Bartlett R, Stokes L, Jalilian I, Peranec M, Sluyter V, Curtis BL, Skarratt KK, Skora A, Bakhsh T, Seavers A, McArthur JD, Dowton M, Sluyter R
Physiological genomics46(14)512-22(2014 Jul)

Functional expression of the damage-associated molecular pattern receptor P2X7 on canine kidney epithelial cells.
Jalilian I, Spildrejorde M, Seavers A, Curtis BL, McArthur JD, Sluyter R
Veterinary immunology and immunopathology150(3-4)228-33(2012 Dec)

Cloning and pharmacological characterization of the dog P2X7 receptor.
Roman S, Cusdin FS, Fonfria E, Goodwin JA, Reeves J, Lappin SC, Chambers L, Walter DS, Clay WC, Michel AD
British journal of pharmacology158(6)1513-26(2009 Nov)

Mechanism of action of species-selective P2X(7) receptor antagonists.
Michel AD, Ng SW, Roman S, Clay WC, Dean DK, Walter DS
British journal of pharmacology156(8)1312-25(2009 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We identified three candidate genes that were highly associated with glioma susceptibility: CAMKK2, P2RX7 and DENR. CAMKK2 showed reduced expression in both canine and human brain tumors, and a non-synonymous variant in P2RX7, previously demonstrated to have a 50% decrease in receptor function, was also associated with disease.
Title: Utilizing the Dog Genome in the Search for Novel Candidate Genes Involved in Glioma Development-Genome Wide Association Mapping followed by Targeted Massive Parallel Sequencing Identifies a Strongly Associated Locus.

The canine P2RX7 gene encoded four missense polymorphisms.
Title: R270C polymorphism leads to loss of function of the canine P2X7 receptor.

The MDCK epithelial cell line expresses functional P2X7, as well as Toll-like receptor 4 and molecules associated with the NALP3 inflammasome.
Title: Functional expression of the damage-associated molecular pattern receptor P2X7 on canine kidney epithelial cells.

The dog P2X7 receptor was 595 amino acids long and exhibited high homology (>70%) to the human and rodent orthologues although it contained an additional threonine at position 284 and an amino acid deletion at position 538
Title: Cloning and pharmacological characterization of the dog P2X7 receptor.

Report mechanism of action of species-selective P2X(7) receptor antagonists.
Title: Mechanism of action of species-selective P2X(7) receptor antagonists.

The following P2RX7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the P2RX7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe33868	NM_001113456.1 Latest version!	Canis lupus familiaris purinergic receptor P2X 7 (P2RX7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OCe43185	XM_014107713.2 Latest version!	Canis lupus familiaris purinergic receptor P2X 7 (P2RX7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OCe43186	XM_014107714.1 Latest version!	Canis lupus familiaris purinergic receptor P2X, ligand gated ion channel, 7 (P2RX7), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
View Old Accession Versions >>
OCe43185	XM_014107713.1	Canis lupus familiaris purinergic receptor P2X, ligand gated ion channel, 7 (P2RX7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

P2RX7 ( NM_001113456.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001106927.1 Canis lupus familiaris purinergic receptor P2X 7 (P2RX7), mRNA.

Price & Availability↑

CloneID	OCe33868
Clone ID Related Accession (Same CDS sequence)	NM_001113456.1
Accession Version	NM_001113456.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1788bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Canis lupus familiaris(dog)
Product	P2X purinoceptor 7
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EU334661.1. On Jan 15, 2008 this sequence version replaced XM_534669.2. ##Evidence-Data-START## Transcript exon combination :: EU334661.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA103936002 [ECO:0000348] ##Evidence-Data-END##

ATGTCTGCCT GCTGCAGCTG TAACGATATT TTCCAGTATG AGACAAACAA AATCATCCGG 
ATCCAGAGCA TGAATTATGG CACCATTAAG TGGATCTTCC ATGTGATCAT CTTTTCTTAC 
ATCAGCTTCG CTTTAATCAG TGACAAGCGG TACCAACAGA AAGAGCCTCT TATCAGTTCT 
GTACACACCA AAGTGAAAGG GACAGCAGAG GTGAAAATGG AGATATTGGA GAATGGAATC 
AAGAAGATGG TGTCTACTGT CTTTGACACT GCGGATTACA CCTTCCCCTT GCAGGGGAAC 
TCCTTCTTCG TGATGACAAA CTTTCTCAAG ACAGAAGGCC AACAGCAGGG GTTCTGTCCT 
GAGTTTCCCA CCCGCAGGAC ACTCTGTTCC AATGACTGGG GCTGTAAAAA AGGATGGATG 
GACCCACAAA GCAAGGGGAT CCAGACCGGA AGGTGTATAG AGTATAAAGG GAAGCAGAAG 
ACTTGTGAAG TCTCCGCCTG GTGTCCCATC GAGGCAGTGG AAGAGGCCCC CCGGCCTGCA 
CTCTTGAACG GTGCTGAAAA TTTCACTGTG CTCATCAAGA ATAACATTGA CTTCCCTGGC 
CACAACTACA CCACGAGAAA CATCTTGCCA GATATAAACA TCACTTGTAC CTTTCACAAG 
ACTCAGAACC CACAGTGTCC CATTTTTCGA TTAGGAGATA TCTTCCAACA AACAGGAGAT 
AATTTTTCAG ATGTGGCAAT TCAGGGAGGG ATCATGGGCA TTGAGATCCA CTGGGACTGC 
AACTTAGACA GTTGGTTCCA TCATTGCCGT CCAAAATACA GTTTCCGCCG CCTTGATGAC 
AAGAACACCA CCACTGAGTC CTTGTACCCT GGATACAACT TCAGATACGC CAAGTACTAT 
AAGGAAAACA ACGTTGAAAA ACGGACTCTG ATAAAAGTCT TTGGGATCCG TTTTGACATC 
CTGGTTTTTG GCACCGGAGG AAAATTTGAC ATTATCCAGT TGGTCGTGTA CATTGGCTCA 
ACCCTCTCCT ACTTTGGTTT GGCCACTCTG TTCATTGACT TTCTCATCAA CACTTACTCA 
AGTAAATGCT GTCGATCCCA CATTTATCCC TGTTTCAAGT GCTGTGAATA CTGTGCAGTC 
AATGAATACT ACTACAAGAA GAAGTGTGAG ACCATTGTGG AGCCAAAGCC GACATTAAAA 
TATGTGTCCT TTGTGGATGA AGCCCACATT AGGATGGTGG ACCAACAGCT GCTTCGGAGA 
CGTCTACAAG ATGTCGAAGG TGAAGAAGTC CCAAGACCCT CAATGGAATT CACAGACTTG 
TCCAGGCTGT CCCTGTCTCT CCATGACCTG TCCCCTATTC CTGGACAATC AGAGGAGATG 
CAGCTGTTAA GTGAAGAAGT GACCCCTAGA TCCAGCAACA GCCCAGACTG GTGCCAGTGT 
GGACACTGCC TCCCATCCCA GCTCCCCGAG AGCCAGCGAT GCCTGGAAGA GCTGTGCTGT 
CGGAAAAAGG CTGGTGCCTG CATTACCACC TCAGAGCCGT TCAGGAAGCT CATCTTGTCC 
CGACAGGTCC TGCAGTTCCT CCTGCTCTAC CAGGAGCCCT TGCTGGTGCT GGATGAAAAC 
TCCAACAGCC GGCTGCGGCA CTGTGCCTAC AGGTGCTACA CCACCTGGCG CTTTGGCTCC 
CAGGACCTAG CCGACTTTGC CATCCTGCCC AGCTGCTGCC GCTGGAGGAT CCGGAGAGAA 
TTTCCCAAGA GTGAAGGCCA GTACAGTGGC TTCAGGAGTC CTTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106927.1
CDS	1..1788
Translation	MSACCSCNDIFQYETNKIIRIQSMNYGTIKWIFHVIIFSYISFALISDKRYQQKEPLISSVHTKVKGTAEVKMEILENGIKKMVSTVFDTADYTFPLQGNSFFVMTNFLKTEGQQQGFCPEFPTRRTLCSNDWGCKKGWMDPQSKGIQTGRCIEYKGKQKTCEVSAWCPIEAVEEAPRPALLNGAENFTVLIKNNIDFPGHNYTTRNILPDINITCTFHKTQNPQCPIFRLGDIFQQTGDNFSDVAIQGGIMGIEIHWDCNLDSWFHHCRPKYSFRRLDDKNTTTESLYPGYNFRYAKYYKENNVEKRTLIKVFGIRFDILVFGTGGKFDIIQLVVYIGSTLSYFGLATLFIDFLINTYSSKCCRSHIYPCFKCCEYCAVNEYYYKKKCETIVEPKPTLKYVSFVDEAHIRMVDQQLLRRRLQDVEGEEVPRPSMEFTDLSRLSLSLHDLSPIPGQSEEMQLLSEEVTPRSSNSPDWCQCGHCLPSQLPESQRCLEELCCRKKAGACITTSEPFRKLILSRQVLQFLLLYQEPLLVLDENSNSRLRHCAYRCYTTWRFGSQDLADFAILPSCCRWRIRREFPKSEGQYSGFRSPY

Target ORF information:

RefSeq Version	NM_001113456.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris purinergic receptor P2X 7 (P2RX7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113456.1

ORF Insert Sequence:

ATGTCTGCCT GCTGCAGCTG TAACGATATT TTCCAGTATG AGACAAACAA AATCATCCGG 
ATCCAGAGCA TGAATTATGG CACCATTAAG TGGATCTTCC ATGTGATCAT CTTTTCTTAC 
ATCAGCTTCG CTTTAATCAG TGACAAGCGG TACCAACAGA AAGAGCCTCT TATCAGTTCT 
GTACACACCA AAGTGAAAGG GACAGCAGAG GTGAAAATGG AGATATTGGA GAATGGAATC 
AAGAAGATGG TGTCTACTGT CTTTGACACT GCGGATTACA CCTTCCCCTT GCAGGGGAAC 
TCCTTCTTCG TGATGACAAA CTTTCTCAAG ACAGAAGGCC AACAGCAGGG GTTCTGTCCT 
GAGTTTCCCA CCCGCAGGAC ACTCTGTTCC AATGACTGGG GCTGTAAAAA AGGATGGATG 
GACCCACAAA GCAAGGGGAT CCAGACCGGA AGGTGTATAG AGTATAAAGG GAAGCAGAAG 
ACTTGTGAAG TCTCCGCCTG GTGTCCCATC GAGGCAGTGG AAGAGGCCCC CCGGCCTGCA 
CTCTTGAACG GTGCTGAAAA TTTCACTGTG CTCATCAAGA ATAACATTGA CTTCCCTGGC 
CACAACTACA CCACGAGAAA CATCTTGCCA GATATAAACA TCACTTGTAC CTTTCACAAG 
ACTCAGAACC CACAGTGTCC CATTTTTCGA TTAGGAGATA TCTTCCAACA AACAGGAGAT 
AATTTTTCAG ATGTGGCAAT TCAGGGAGGG ATCATGGGCA TTGAGATCCA CTGGGACTGC 
AACTTAGACA GTTGGTTCCA TCATTGCCGT CCAAAATACA GTTTCCGCCG CCTTGATGAC 
AAGAACACCA CCACTGAGTC CTTGTACCCT GGATACAACT TCAGATACGC CAAGTACTAT 
AAGGAAAACA ACGTTGAAAA ACGGACTCTG ATAAAAGTCT TTGGGATCCG TTTTGACATC 
CTGGTTTTTG GCACCGGAGG AAAATTTGAC ATTATCCAGT TGGTCGTGTA CATTGGCTCA 
ACCCTCTCCT ACTTTGGTTT GGCCACTCTG TTCATTGACT TTCTCATCAA CACTTACTCA 
AGTAAATGCT GTCGATCCCA CATTTATCCC TGTTTCAAGT GCTGTGAATA CTGTGCAGTC 
AATGAATACT ACTACAAGAA GAAGTGTGAG ACCATTGTGG AGCCAAAGCC GACATTAAAA 
TATGTGTCCT TTGTGGATGA AGCCCACATT AGGATGGTGG ACCAACAGCT GCTTCGGAGA 
CGTCTACAAG ATGTCGAAGG TGAAGAAGTC CCAAGACCCT CAATGGAATT CACAGACTTG 
TCCAGGCTGT CCCTGTCTCT CCATGACCTG TCCCCTATTC CTGGACAATC AGAGGAGATG 
CAGCTGTTAA GTGAAGAAGT GACCCCTAGA TCCAGCAACA GCCCAGACTG GTGCCAGTGT 
GGACACTGCC TCCCATCCCA GCTCCCCGAG AGCCAGCGAT GCCTGGAAGA GCTGTGCTGT 
CGGAAAAAGG CTGGTGCCTG CATTACCACC TCAGAGCCGT TCAGGAAGCT CATCTTGTCC 
CGACAGGTCC TGCAGTTCCT CCTGCTCTAC CAGGAGCCCT TGCTGGTGCT GGATGAAAAC 
TCCAACAGCC GGCTGCGGCA CTGTGCCTAC AGGTGCTACA CCACCTGGCG CTTTGGCTCC 
CAGGACCTAG CCGACTTTGC CATCCTGCCC AGCTGCTGCC GCTGGAGGAT CCGGAGAGAA 
TTTCCCAAGA GTGAAGGCCA GTACAGTGGC TTCAGGAGTC CTTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

P2RX7 ( XM_014107713.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963188.1 Canis lupus familiaris purinergic receptor P2X, ligand gated ion channel, 7 (P2RX7), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe43185
Clone ID Related Accession (Same CDS sequence)	XM_014107713.1 , XM_014107713.2
Accession Version	XM_014107713.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	P2X purinoceptor 7 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCCT GCTGCAGCTG TAACGATATT TTCCAGTATG AGACAAACAA AATCATCCGG 
ATCCAGAGCA TGAATTATGG CACCATTAAG TGGATCTTCC ATGTGATCAT CTTTTCTTAC 
ATCAGCTTCG CTTTAATCAG TGACAAGCGG TACCAACAGA AAGAGCCTCT TATCAGTTCT 
GTACACACCA AAGTGAAAGG GACAGCAGAG GTGAAAATGG AGATATTGGA GAATGGAATC 
AAGAAGATGG TGTCTACTGT CTTTGACACT GCGGATTACA CCTTCCCCTT GCAGGGGAAC 
TCCTTCCTCG TGATGACAAA CTTTCTCAAG ACAGAAGGCC AACAGCAGGG GTTCTGTCCT 
GAGTTTCCCA CCCGCAGGAC ACTCTGTTCC AATGACTGGG GCTGTAAAAA AGGATGGATG 
GACCCACAAA GCAAGGGGAT CCAGACCGGA AGGTGTATAG AGTATAAAGG GAAGCAGAAG 
ACTTGTGAAG TCTCCGCCTG GTGTCCCATC GAGGCAGTGG AAGAGGCCCC CCGGCCTGCA 
CTCTTGAACG GTGCTGAAAA TTTCACTGTG CTCATCAAGA ATAACATTGA CTTCCCTGGC 
CACAACTACA CCACGAGAAA CATCTTGCCA GATATAAACA TCACTTGTAC CTTTCACAAG 
ACTCAGAACC CACAGTGTCC CATTTTTCGA TTAGGAGATA TCTTCCAACA AACAGGAGAT 
AATTTTTCAG ATGTGGCAAT TCAGGGAGGG ATCATGGGCA TTGAGATCCA CTGGGACTGC 
AACTTAGACA GTTGGTTCCA TCATTGCCGT CCAAAATACA GTTTCCGCCG CCTTGATGAC 
AAGAACACCA CCACTGAGTC CTTGTACCCT GGATACAACT TCAGATACGC CAAGTACTAT 
AAGGAAAACA ACGTTGAAAA ACGGACTCTG ATAAAAGTCT TTGGGATCCG TTTTGACATC 
CTGGTTTTTG GCACCGGAGG AAAATTTGAC ATTATCCAGT TGGTCGTGTA CATTGGCTCA 
ACCCTCTCCT ACTTTGGTTT GGCCACTCTG TTCATTGACT TTCTCATCAA CACTTACTCA 
AGTAAATGCT GTCGATCCCA CATTTATCCC TGTTTCAAGT GCTGTGAATA CTGTGCAGTC 
AATGAATACT ACTACAAGAA GAAGTGTGAG ACCATTGTGG AGCCAAAGCC GACATTAAAA 
TATGTGTCCT TTGTGGATGA AGCCCACATT AGGATGGTGG ACCAACAGCT GCTTCGGAGA 
CGTCTACAAG ATGTCGAAGG TGAAGAAGTC CCAATTCTAT ATCATCCTGG TAAAGACATG 
TCCTCCTCAA CTTCTGTATG CAAAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963188.1
CDS	150..1499
Translation	MSACCSCNDIFQYETNKIIRIQSMNYGTIKWIFHVIIFSYISFALISDKRYQQKEPLISSVHTKVKGTAEVKMEILENGIKKMVSTVFDTADYTFPLQGNSFLVMTNFLKTEGQQQGFCPEFPTRRTLCSNDWGCKKGWMDPQSKGIQTGRCIEYKGKQKTCEVSAWCPIEAVEEAPRPALLNGAENFTVLIKNNIDFPGHNYTTRNILPDINITCTFHKTQNPQCPIFRLGDIFQQTGDNFSDVAIQGGIMGIEIHWDCNLDSWFHHCRPKYSFRRLDDKNTTTESLYPGYNFRYAKYYKENNVEKRTLIKVFGIRFDILVFGTGGKFDIIQLVVYIGSTLSYFGLATLFIDFLINTYSSKCCRSHIYPCFKCCEYCAVNEYYYKKKCETIVEPKPTLKYVSFVDEAHIRMVDQQLLRRRLQDVEGEEVPILYHPGKDMSSSTSVCKN

Target ORF information:

RefSeq Version	XM_014107713.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris purinergic receptor P2X, ligand gated ion channel, 7 (P2RX7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107713.1

ORF Insert Sequence:

ATGTCTGCCT GCTGCAGCTG TAACGATATT TTCCAGTATG AGACAAACAA AATCATCCGG 
ATCCAGAGCA TGAATTATGG CACCATTAAG TGGATCTTCC ATGTGATCAT CTTTTCTTAC 
ATCAGCTTCG CTTTAATCAG TGACAAGCGG TACCAACAGA AAGAGCCTCT TATCAGTTCT 
GTACACACCA AAGTGAAAGG GACAGCAGAG GTGAAAATGG AGATATTGGA GAATGGAATC 
AAGAAGATGG TGTCTACTGT CTTTGACACT GCGGATTACA CCTTCCCCTT GCAGGGGAAC 
TCCTTCCTCG TGATGACAAA CTTTCTCAAG ACAGAAGGCC AACAGCAGGG GTTCTGTCCT 
GAGTTTCCCA CCCGCAGGAC ACTCTGTTCC AATGACTGGG GCTGTAAAAA AGGATGGATG 
GACCCACAAA GCAAGGGGAT CCAGACCGGA AGGTGTATAG AGTATAAAGG GAAGCAGAAG 
ACTTGTGAAG TCTCCGCCTG GTGTCCCATC GAGGCAGTGG AAGAGGCCCC CCGGCCTGCA 
CTCTTGAACG GTGCTGAAAA TTTCACTGTG CTCATCAAGA ATAACATTGA CTTCCCTGGC 
CACAACTACA CCACGAGAAA CATCTTGCCA GATATAAACA TCACTTGTAC CTTTCACAAG 
ACTCAGAACC CACAGTGTCC CATTTTTCGA TTAGGAGATA TCTTCCAACA AACAGGAGAT 
AATTTTTCAG ATGTGGCAAT TCAGGGAGGG ATCATGGGCA TTGAGATCCA CTGGGACTGC 
AACTTAGACA GTTGGTTCCA TCATTGCCGT CCAAAATACA GTTTCCGCCG CCTTGATGAC 
AAGAACACCA CCACTGAGTC CTTGTACCCT GGATACAACT TCAGATACGC CAAGTACTAT 
AAGGAAAACA ACGTTGAAAA ACGGACTCTG ATAAAAGTCT TTGGGATCCG TTTTGACATC 
CTGGTTTTTG GCACCGGAGG AAAATTTGAC ATTATCCAGT TGGTCGTGTA CATTGGCTCA 
ACCCTCTCCT ACTTTGGTTT GGCCACTCTG TTCATTGACT TTCTCATCAA CACTTACTCA 
AGTAAATGCT GTCGATCCCA CATTTATCCC TGTTTCAAGT GCTGTGAATA CTGTGCAGTC 
AATGAATACT ACTACAAGAA GAAGTGTGAG ACCATTGTGG AGCCAAAGCC GACATTAAAA 
TATGTGTCCT TTGTGGATGA AGCCCACATT AGGATGGTGG ACCAACAGCT GCTTCGGAGA 
CGTCTACAAG ATGTCGAAGG TGAAGAAGTC CCAATTCTAT ATCATCCTGG TAAAGACATG 
TCCTCCTCAA CTTCTGTATG CAAAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

P2RX7 ( XM_014107713.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963188.1 Canis lupus familiaris purinergic receptor P2X 7 (P2RX7), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe43185
Clone ID Related Accession (Same CDS sequence)	XM_014107713.1 , XM_014107713.2
Accession Version	XM_014107713.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	P2X purinoceptor 7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014107713.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCCT GCTGCAGCTG TAACGATATT TTCCAGTATG AGACAAACAA AATCATCCGG 
ATCCAGAGCA TGAATTATGG CACCATTAAG TGGATCTTCC ATGTGATCAT CTTTTCTTAC 
ATCAGCTTCG CTTTAATCAG TGACAAGCGG TACCAACAGA AAGAGCCTCT TATCAGTTCT 
GTACACACCA AAGTGAAAGG GACAGCAGAG GTGAAAATGG AGATATTGGA GAATGGAATC 
AAGAAGATGG TGTCTACTGT CTTTGACACT GCGGATTACA CCTTCCCCTT GCAGGGGAAC 
TCCTTCCTCG TGATGACAAA CTTTCTCAAG ACAGAAGGCC AACAGCAGGG GTTCTGTCCT 
GAGTTTCCCA CCCGCAGGAC ACTCTGTTCC AATGACTGGG GCTGTAAAAA AGGATGGATG 
GACCCACAAA GCAAGGGGAT CCAGACCGGA AGGTGTATAG AGTATAAAGG GAAGCAGAAG 
ACTTGTGAAG TCTCCGCCTG GTGTCCCATC GAGGCAGTGG AAGAGGCCCC CCGGCCTGCA 
CTCTTGAACG GTGCTGAAAA TTTCACTGTG CTCATCAAGA ATAACATTGA CTTCCCTGGC 
CACAACTACA CCACGAGAAA CATCTTGCCA GATATAAACA TCACTTGTAC CTTTCACAAG 
ACTCAGAACC CACAGTGTCC CATTTTTCGA TTAGGAGATA TCTTCCAACA AACAGGAGAT 
AATTTTTCAG ATGTGGCAAT TCAGGGAGGG ATCATGGGCA TTGAGATCCA CTGGGACTGC 
AACTTAGACA GTTGGTTCCA TCATTGCCGT CCAAAATACA GTTTCCGCCG CCTTGATGAC 
AAGAACACCA CCACTGAGTC CTTGTACCCT GGATACAACT TCAGATACGC CAAGTACTAT 
AAGGAAAACA ACGTTGAAAA ACGGACTCTG ATAAAAGTCT TTGGGATCCG TTTTGACATC 
CTGGTTTTTG GCACCGGAGG AAAATTTGAC ATTATCCAGT TGGTCGTGTA CATTGGCTCA 
ACCCTCTCCT ACTTTGGTTT GGCCACTCTG TTCATTGACT TTCTCATCAA CACTTACTCA 
AGTAAATGCT GTCGATCCCA CATTTATCCC TGTTTCAAGT GCTGTGAATA CTGTGCAGTC 
AATGAATACT ACTACAAGAA GAAGTGTGAG ACCATTGTGG AGCCAAAGCC GACATTAAAA 
TATGTGTCCT TTGTGGATGA AGCCCACATT AGGATGGTGG ACCAACAGCT GCTTCGGAGA 
CGTCTACAAG ATGTCGAAGG TGAAGAAGTC CCAATTCTAT ATCATCCTGG TAAAGACATG 
TCCTCCTCAA CTTCTGTATG CAAAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963188.1
CDS	217..1566
Translation	MSACCSCNDIFQYETNKIIRIQSMNYGTIKWIFHVIIFSYISFALISDKRYQQKEPLISSVHTKVKGTAEVKMEILENGIKKMVSTVFDTADYTFPLQGNSFLVMTNFLKTEGQQQGFCPEFPTRRTLCSNDWGCKKGWMDPQSKGIQTGRCIEYKGKQKTCEVSAWCPIEAVEEAPRPALLNGAENFTVLIKNNIDFPGHNYTTRNILPDINITCTFHKTQNPQCPIFRLGDIFQQTGDNFSDVAIQGGIMGIEIHWDCNLDSWFHHCRPKYSFRRLDDKNTTTESLYPGYNFRYAKYYKENNVEKRTLIKVFGIRFDILVFGTGGKFDIIQLVVYIGSTLSYFGLATLFIDFLINTYSSKCCRSHIYPCFKCCEYCAVNEYYYKKKCETIVEPKPTLKYVSFVDEAHIRMVDQQLLRRRLQDVEGEEVPILYHPGKDMSSSTSVCKN

Target ORF information:

RefSeq Version	XM_014107713.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris purinergic receptor P2X 7 (P2RX7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107713.2

ORF Insert Sequence:

ATGTCTGCCT GCTGCAGCTG TAACGATATT TTCCAGTATG AGACAAACAA AATCATCCGG 
ATCCAGAGCA TGAATTATGG CACCATTAAG TGGATCTTCC ATGTGATCAT CTTTTCTTAC 
ATCAGCTTCG CTTTAATCAG TGACAAGCGG TACCAACAGA AAGAGCCTCT TATCAGTTCT 
GTACACACCA AAGTGAAAGG GACAGCAGAG GTGAAAATGG AGATATTGGA GAATGGAATC 
AAGAAGATGG TGTCTACTGT CTTTGACACT GCGGATTACA CCTTCCCCTT GCAGGGGAAC 
TCCTTCCTCG TGATGACAAA CTTTCTCAAG ACAGAAGGCC AACAGCAGGG GTTCTGTCCT 
GAGTTTCCCA CCCGCAGGAC ACTCTGTTCC AATGACTGGG GCTGTAAAAA AGGATGGATG 
GACCCACAAA GCAAGGGGAT CCAGACCGGA AGGTGTATAG AGTATAAAGG GAAGCAGAAG 
ACTTGTGAAG TCTCCGCCTG GTGTCCCATC GAGGCAGTGG AAGAGGCCCC CCGGCCTGCA 
CTCTTGAACG GTGCTGAAAA TTTCACTGTG CTCATCAAGA ATAACATTGA CTTCCCTGGC 
CACAACTACA CCACGAGAAA CATCTTGCCA GATATAAACA TCACTTGTAC CTTTCACAAG 
ACTCAGAACC CACAGTGTCC CATTTTTCGA TTAGGAGATA TCTTCCAACA AACAGGAGAT 
AATTTTTCAG ATGTGGCAAT TCAGGGAGGG ATCATGGGCA TTGAGATCCA CTGGGACTGC 
AACTTAGACA GTTGGTTCCA TCATTGCCGT CCAAAATACA GTTTCCGCCG CCTTGATGAC 
AAGAACACCA CCACTGAGTC CTTGTACCCT GGATACAACT TCAGATACGC CAAGTACTAT 
AAGGAAAACA ACGTTGAAAA ACGGACTCTG ATAAAAGTCT TTGGGATCCG TTTTGACATC 
CTGGTTTTTG GCACCGGAGG AAAATTTGAC ATTATCCAGT TGGTCGTGTA CATTGGCTCA 
ACCCTCTCCT ACTTTGGTTT GGCCACTCTG TTCATTGACT TTCTCATCAA CACTTACTCA 
AGTAAATGCT GTCGATCCCA CATTTATCCC TGTTTCAAGT GCTGTGAATA CTGTGCAGTC 
AATGAATACT ACTACAAGAA GAAGTGTGAG ACCATTGTGG AGCCAAAGCC GACATTAAAA 
TATGTGTCCT TTGTGGATGA AGCCCACATT AGGATGGTGG ACCAACAGCT GCTTCGGAGA 
CGTCTACAAG ATGTCGAAGG TGAAGAAGTC CCAATTCTAT ATCATCCTGG TAAAGACATG 
TCCTCCTCAA CTTCTGTATG CAAAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

P2RX7 ( XM_014107714.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963189.1 Canis lupus familiaris purinergic receptor P2X, ligand gated ion channel, 7 (P2RX7), transcript variant X2, mRNA.

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CloneID	OCe43186
Clone ID Related Accession (Same CDS sequence)	XM_014107714.1
Accession Version	XM_014107714.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	P2X purinoceptor 7 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCCT GCTGCAGCTG TAACGATATT TTCCAGTATG AGACAAACAA AATCATCCGG 
ATCCAGAGCA TGAATTATGG CACCATTAAG TGGATCTTCC ATGTGATCAT CTTTTCTTAC 
ATCAGCTTCG CTTTAATCAG TGACAAGCGG TACCAACAGA AAGAGCCTCT TATCAGTTCT 
GTACACACCA AAGTGAAAGG GACAGCAGAG GTGAAAATGG AGATATTGGA GAATGGAATC 
AAGAAGATGG TGTCTACTGT CTTTGACACT GCGGATTACA CCTTCCCCTT GCAGGGGAAC 
TCCTTCCTCG TGATGACAAA CTTTCTCAAG ACAGAAGGCC AACAGCAGGG GTTCTGTCCT 
GAGTTTCCCA CCCGCAGGAC ACTCTGTTCC AATGACTGGG GCTGTAAAAA AGGATGGATG 
GACCCACAAA GCAAGGGGAT CCAGACCGGA AGGTGTATAG AGTATAAAGG GAAGCAGAAG 
ACTTGTGAAG TCTCCGCCTG GTGTCCCATC GAGGCAGTGG AAGAGGCCCC CCGGCCTGCA 
CTCTTGAACG GTGCTGAAAA TTTCACTGTG CTCATCAAGA ATAACATTGA CTTCCCTGGC 
CACAACTACA CCACGAGAAA CATCTTGCCA GATATAAACA TCACTTGTAC CTTTCACAAG 
ACTCAGAACC CACAGTGTCC CATTTTTCGA TTAGGAGATA TCTTCCAACA AACAGGAGAT 
AATTTTTCAG ATGTGGCAAT TCAGGGAGGG ATCATGGGCA TTGAGATCCA CTGGGACTGC 
AACTTAGACA GTTGGTTCCA TCATTGCCGT CCAAAATACA GTTTCCGCCG CCTTGATGAC 
AAGAACACCA CCACTGAGTC CTTGTACCCT GGATACAACT TCAGATACGC CAAGTACTAT 
AAGGAAAACA ACGTTGAAAA ACGGACTCTG ATAAAAGTCT TTGGGATCCG TTTTGACATC 
CTGGTTTTTG GCACCGGAGG AAAATTTGAC ATTATCCAGT TGGTCGTGTA CATTGGCTCA 
ACCCTCTCCT ACTTTGGTTT GGCCACTCTG TTCATTGACT TTCTCATCAA CACTTACTCA 
AGTAAATGCT GTCGATCCCA CATTTATCCC TGTTTCAAGT GCTGTGAATA CTGTGCAGTC 
AATGAATACT ACTACAAGAA GAAGTGTGAG ACCATTGTGG AGCCAAAGCC GACATTAAAA 
TATGTGTCCT TTGTGGATGA AGCCCACATT AGGATGGTGG ACCAACAGCT GCTTCGGAGA 
CGTCTACAAG ATGTCGAAGG TGAAGAAGTC CCAGCACTGG GGATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963189.1
CDS	150..1457
Translation	MSACCSCNDIFQYETNKIIRIQSMNYGTIKWIFHVIIFSYISFALISDKRYQQKEPLISSVHTKVKGTAEVKMEILENGIKKMVSTVFDTADYTFPLQGNSFLVMTNFLKTEGQQQGFCPEFPTRRTLCSNDWGCKKGWMDPQSKGIQTGRCIEYKGKQKTCEVSAWCPIEAVEEAPRPALLNGAENFTVLIKNNIDFPGHNYTTRNILPDINITCTFHKTQNPQCPIFRLGDIFQQTGDNFSDVAIQGGIMGIEIHWDCNLDSWFHHCRPKYSFRRLDDKNTTTESLYPGYNFRYAKYYKENNVEKRTLIKVFGIRFDILVFGTGGKFDIIQLVVYIGSTLSYFGLATLFIDFLINTYSSKCCRSHIYPCFKCCEYCAVNEYYYKKKCETIVEPKPTLKYVSFVDEAHIRMVDQQLLRRRLQDVEGEEVPALGI

Target ORF information:

RefSeq Version	XM_014107714.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris purinergic receptor P2X, ligand gated ion channel, 7 (P2RX7), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107714.1

ORF Insert Sequence:

ATGTCTGCCT GCTGCAGCTG TAACGATATT TTCCAGTATG AGACAAACAA AATCATCCGG 
ATCCAGAGCA TGAATTATGG CACCATTAAG TGGATCTTCC ATGTGATCAT CTTTTCTTAC 
ATCAGCTTCG CTTTAATCAG TGACAAGCGG TACCAACAGA AAGAGCCTCT TATCAGTTCT 
GTACACACCA AAGTGAAAGG GACAGCAGAG GTGAAAATGG AGATATTGGA GAATGGAATC 
AAGAAGATGG TGTCTACTGT CTTTGACACT GCGGATTACA CCTTCCCCTT GCAGGGGAAC 
TCCTTCCTCG TGATGACAAA CTTTCTCAAG ACAGAAGGCC AACAGCAGGG GTTCTGTCCT 
GAGTTTCCCA CCCGCAGGAC ACTCTGTTCC AATGACTGGG GCTGTAAAAA AGGATGGATG 
GACCCACAAA GCAAGGGGAT CCAGACCGGA AGGTGTATAG AGTATAAAGG GAAGCAGAAG 
ACTTGTGAAG TCTCCGCCTG GTGTCCCATC GAGGCAGTGG AAGAGGCCCC CCGGCCTGCA 
CTCTTGAACG GTGCTGAAAA TTTCACTGTG CTCATCAAGA ATAACATTGA CTTCCCTGGC 
CACAACTACA CCACGAGAAA CATCTTGCCA GATATAAACA TCACTTGTAC CTTTCACAAG 
ACTCAGAACC CACAGTGTCC CATTTTTCGA TTAGGAGATA TCTTCCAACA AACAGGAGAT 
AATTTTTCAG ATGTGGCAAT TCAGGGAGGG ATCATGGGCA TTGAGATCCA CTGGGACTGC 
AACTTAGACA GTTGGTTCCA TCATTGCCGT CCAAAATACA GTTTCCGCCG CCTTGATGAC 
AAGAACACCA CCACTGAGTC CTTGTACCCT GGATACAACT TCAGATACGC CAAGTACTAT 
AAGGAAAACA ACGTTGAAAA ACGGACTCTG ATAAAAGTCT TTGGGATCCG TTTTGACATC 
CTGGTTTTTG GCACCGGAGG AAAATTTGAC ATTATCCAGT TGGTCGTGTA CATTGGCTCA 
ACCCTCTCCT ACTTTGGTTT GGCCACTCTG TTCATTGACT TTCTCATCAA CACTTACTCA 
AGTAAATGCT GTCGATCCCA CATTTATCCC TGTTTCAAGT GCTGTGAATA CTGTGCAGTC 
AATGAATACT ACTACAAGAA GAAGTGTGAG ACCATTGTGG AGCCAAAGCC GACATTAAAA 
TATGTGTCCT TTGTGGATGA AGCCCACATT AGGATGGTGG ACCAACAGCT GCTTCGGAGA 
CGTCTACAAG ATGTCGAAGG TGAAGAAGTC CCAGCACTGG GGATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Utilizing the Dog Genome in the Search for Novel Candidate Genes Involved in Glioma Development-Genome Wide Association Mapping followed by Targeted Massive Parallel Sequencing Identifies a Strongly Associated Locus.
PLoS genetics12(5)e1006000(2016 May)
Truv? K,Dickinson P,Xiong A,York D,Jayashankar K,Pielberg G,Koltookian M,Mur?n E,Fuxelius HH,Weishaupt H,Swartling FJ,Andersson G,Hedhammar ?,Bongcam-Rudloff E,Forsberg-Nilsson K,Bannasch D,Lindblad-Toh K

R270C polymorphism leads to loss of function of the canine P2X7 receptor.
Physiological genomics46(14)512-22(2014 Jul)
Spildrejorde M,Bartlett R,Stokes L,Jalilian I,Peranec M,Sluyter V,Curtis BL,Skarratt KK,Skora A,Bakhsh T,Seavers A,McArthur JD,Dowton M,Sluyter R

Functional expression of the damage-associated molecular pattern receptor P2X7 on canine kidney epithelial cells.
Veterinary immunology and immunopathology150(3-4)228-33(2012 Dec)
Jalilian I,Spildrejorde M,Seavers A,Curtis BL,McArthur JD,Sluyter R

Cloning and pharmacological characterization of the dog P2X7 receptor.
British journal of pharmacology158(6)1513-26(2009 Nov)
Roman S,Cusdin FS,Fonfria E,Goodwin JA,Reeves J,Lappin SC,Chambers L,Walter DS,Clay WC,Michel AD

Mechanism of action of species-selective P2X(7) receptor antagonists.
British journal of pharmacology156(8)1312-25(2009 Apr)
Michel AD,Ng SW,Roman S,Clay WC,Dean DK,Walter DS

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P2RX7 cDNA ORF clone, Canis lupus familiaris(dog)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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