TJP1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	TJP1
Entrez Gene ID	403752
Full Name	tight junction protein 1
Synonyms	ZO-1,ZO1
General protein information	Preferred Names tight junction protein 1 Names tight junction protein ZO-1 tight junction protein 1 (zona occludens 1) zonula occludens protein 1
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001003140.1	NP_001003140.1	tight junction protein ZO-1

Pathways from BioSystems

Homologies

Homo sapiens (human)	TJP1	NP_003248.3
	TJP1	XP_002804738.1
Canis lupus familiaris (dog)	TJP1	NP_001003140.1
Rattus norvegicus (Norway rat)	Tjp1	NP_001099736.1
Danio rerio (zebrafish)	LOC565459	XP_003201509.2
Bos taurus (cattle)	TJP1	XP_002696696.3
Gallus gallus (chicken)	TJP1	XP_413773.4
Danio rerio (zebrafish)	tjp1a	XP_005168990.1
Pan troglodytes (chimpanzee)	TJP1	XP_001163120.1
Mus musculus (house mouse)	Tjp1	NP_033412.2
Xenopus tropicalis (tropical clawed frog)	tjp1	XP_004917990.1

Gene Ontology
Bibliography

Related articles in PubMed

Expression of claudins, occludin, junction adhesion molecule A and zona occludens 1 in canine organs.
Ahn C, Shin DH, Lee D, Kang SM, Seok JH, Kang HY, Jeung EB
Molecular medicine reports14(4)3697-703(2016 Oct)

Tight junction proteins in the canine epidermis: a pilot study on their distribution in normal and in high IgE-producing canines.
Roussel AJ, Bruet V, Marsella R, Knol AC, Bourdeau PJ
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire79(1)46-51(2015 Jan)

ZO-1 knockout by TALEN-mediated gene targeting in MDCK cells: involvement of ZO-1 in the regulation of cytoskeleton and cell shape.
Tokuda S, Higashi T, Furuse M
PloS one9(8)e104994(2014)

Injury-triggered Akt phosphorylation of Cx43: a ZO-1-driven molecular switch that regulates gap junction size.
Dunn CA, Lampe PD
Journal of cell science127(Pt 2)455-64(2014 Jan)

ZO-1 recruitment to ?-catenin--a novel mechanism for coupling the assembly of tight junctions to adherens junctions.
Maiers JL, Peng X, Fanning AS, DeMali KA
Journal of cell science126(Pt 17)3904-15(2013 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In the present study, organspecific expression of the tight junction proteins, claudin, occludin, junction adhesion molecule A and zona occludens 1 was examined in the canine duodenum, lung, liver and kidney.
Title: Expression of claudins, occludin, junction adhesion molecule A and zona occludens 1 in canine organs.

Occludin and ZO-1 expression remained unchanged between atopic and clinically normal dogs.
Title: Tight junction proteins in the canine epidermis: a pilot study on their distribution in normal and in high IgE-producing canines.

ZO-1 knockout induced striking changes in myosin organization at cell-cell contacts and disrupted the localization of tight junction proteins.
Title: ZO-1 knockout by TALEN-mediated gene targeting in MDCK cells: involvement of ZO-1 in the regulation of cytoskeleton and cell shape.

Akt-dependent phosphorylation of Serine 373 increases gap junction size and communication by completely eliminating the interaction between Cx43 and ZO-1.
Title: Injury-triggered Akt phosphorylation of Cx43: a ZO-1-driven molecular switch that regulates gap junction size.

our study identifies alpha-catenin binding to ZO-1 as a new mechanism for coupling the assembly of the epithelial barrier to cell-to-cell adhesion.
Title: ZO-1 recruitment to ?-catenin--a novel mechanism for coupling the assembly of tight junctions to adherens junctions.

The following TJP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TJP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe00362	NM_001003140.1 Latest version!	Canis lupus familiaris tight junction protein 1 (TJP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TJP1 ( NM_001003140.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001003140.1 Canis lupus familiaris tight junction protein 1 (TJP1), mRNA.

Price & Availability↑

CloneID	OCe00362
Clone ID Related Accession (Same CDS sequence)	NM_001003140.1
Accession Version	NM_001003140.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Canis lupus familiaris(dog)
Product	tight junction protein ZO-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U55935.1. On Jan 12, 2005 this sequence version replaced XM_536171.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U55935.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA103936001, SAMEA103936002 [ECO:0000350] ##Evidence-Data-END##

ATGTCCGCCA GAGCTGCGGC CGCCAAGAAC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACACACAG TGACGCTTCA CAGGGCACCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGAAGAGATA ACCCTCATTT TCAGAGTGGA GAAACTTCCA TAGTAATTTC AGATGTGCTG 
AAAGGGGGAC CTGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAATGGA 
GTTTCAATGG ATAATGTTGA ACATGCGTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAG 
AATGCAAAAA TTACCATTCG GAGAAAGAAG AAAGTTCAGA TACCAGTTAG TCGTCCTGAT 
CCTGAACCAG TGTCTGAGAA TGAAGATAGT TATGATGAGG AAGTCCATGA CCCAAGAAGC 
AGCCGTGGTG GTCTGGTCAG TAGAAGGAGT GAGAAGAGTT GGGCAAGGGA CAGGAGTGCA 
AGCAGAGAGA GGAGTTTGTC TCCACGGTCT GATAGGCGAT CTGTGGCCTC CAGTCAACCT 
CCCAAACCTA CCAAAGTCAC ATTGGTGAAA TCCCGGAAAA ATGAAGAATA TGGTCTTCGA 
TTGGCCAGCC ATATATTTGT AAAGGAAATT TCACAAGATA GTTTGGCAGC AAGAGATGGC 
AATATTCAAG AAGGCGATGT TGTATTAAAG ATAAATGGTA CTGTGACAGA AAATATGTCA 
CTGACAGATG CAAAGACTTT GATAGAAAGA TCTAAAGGCA AGTTAAAAAT GGTAGTTCAA 
AGGGATGAAC GGGCTACCCT ATTGAATGTC CCTGATCTTT CTGACAGTAT CCACTCTGCT 
AATGCTTCTG AGAGAGATGA TATTTCAGAA ATTCAGTCAC TGGCATCAGA TCATTCTGGT 
CGATCACATG ATAGACCTCC CCGCCACAGC CGGTCACGGT CACCTGACCA ACGATCAGAG 
CCTTCTGATC ATTCCAGACA CTCTCCACAG CAGCCCAGCA GTGGCAGTCT CCGGAGCAGA 
GAGGAAGAGA GAATTTCTAA ACCTGGGGCT GTCTCAACTC CTGTAAAGCA TGCAGATGAT 
CATACACATA AAACAGTAGA AGAAGTTGTA GTTGAGAGAA ATGAGAAACA AGCACCCAGT 
CTTCCAGAAC CAAAGCCTGT GTATGCTCAA GTTGGACAAC CAGATGTGGA TTTACCTGTC 
AGTCCATCTG ATGGCGTCCT ACCTAATTCA ACTCATGAAG ATGGGATTCT CCGGCCCAGC 
ATGAAATTGG TAAAATTCAG AAAAGGAGAT AGTGTGGGCC TGCGCCTGGC TGGTGGAAAT 
GATGTTGGAA TATTTGTAGC TGGTGTTCTA GAAGATAGCC CTGCAGCCAA AGAAGGCTTA 
GAAGAGGGTG ATCAAATTCT CAGGGTAAAC AATGTAGATT TCACAAACAT CATAAGAGAA 
GAAGCCGTCC TTTTCCTACT CGACCTCCCT AAAGGAGAAG AAGTGACCAT ATTGGCTCAG 
AAGAAGAAAG ATGTTTATCG TCGCATTGTA GAATCAGATG TGGGAGATTC TTTCTATATT 
AGAACCCACT TTGAATATGA AAAGGAATCT CCCTACGGAC TTAGTTTTAA CAAAGGAGAG 
GTGTTTCGTG TTGTGGATAC CTTGTACAAT GGGAAACTAG GCTCTTGGCT CGCTATTCGA 
ATTGGCAAAA ATCATAAAGA AGTAGAACGA GGCATCATCC CTAATAAGAA CAGGGCTGAG 
CAGTTAGCCA GTGTACAGTA CACACTTCCA AAAACAGCTG GGGGAGACCG TGCTGACTTC 
TGGAGATTCC GGGGTCTTCG CAGCTCCAAG AGAAATCTTC GAAAGAGTAG AGAGGATTTG 
TCAGCTCAGC CAGTTCAAAC AAAGTTTCCA GCCTATGAAA GAGTTGTTCT TCGAGAAGCT 
GGATTTCTGA GGCCTGTAAC TATCTTTGGA CCCATAGCTG ATGTTGCCAG AGAAAAGCTG 
GCAAGAGAAG AACCAGATAT TTATCAGATT GCAAAAAGTG AACCACGAGA TGCTGGAACT 
GATCAACGTA GCTCTGGTAT TATTCGTCTT CATACAATAA AACAAATAAT AGATCAGGAC 
AAACATGCTT TATTAGATGT AACACCAAAT GCAGTTGATC GTCTGAATTA TGCCCAGTGG 
TATCCAATTG TAGTATTCCT TAACCCTGAT TCTAAGCAAG GTGTGAAAAC TATGAGGATG 
AGGCTATGCC CAGAATCTCG AAAAAGTGCC AGGAAATTAT ATGAACGGTC TCATAAACTT 
CGTAAAAATA ATCACCATCT TTTTACAACT ACAATTAACT TAAATTCAAT GAATGATGGT 
TGGTATGGTG CATTGAAAGA AGCAATTCAG CAGCAACAGA ACCAGTTGGT ATGGGTTTCT 
GAGGGAAAGG CGGATGGTGC TACAAGTGAT GACCTTGATT TGCATGATGA TCGTCTCTCC 
TACCTGTCAG CCCCAGGTAG TGAATACTCA ATGTATAGCA CGGACAGTAG ACACACTTCT 
GACTATGAAG ACACAGATAC AGAAGGCGGG GCCTACACTG ATCAAGAACT AGATGAAACT 
CTTAATGATG AGGTTGGGAC TCCACCGGAG TCTGCCATTA CACGGTCCTC TGAGCCTGTA 
AGAGAGGACT CCTCTGGAAT GCATCATGAA AACCAAACAT ATCCTCCTTA TTCACCACAA 
GCGCAGCCAC AACCAATTCA TAGAATAGAC TCCCCTGGAT TTAAAACAGC TTCTCAACAG 
AAAGCAGAAG CCTCATCTCC AGTCCCTTAC CTTTCGCCTG AAACAAACCC AGCATCATCA 
ACCTCTGCTG TTAACCATAA TGTAACTTTA ACTAATGTCA GACTGGAGGG GCCTACCCCA 
GCTCCTTCTA CTTCTTACTC ACCACAAGCT GATTCTTTAA GAACACCAAG CACTGAGGCA 
GCTCACATAA TGCTAAGAGA TCAAGAGCCA TCATTGCCGT CGCATGTAGA GCCAGCAAAG 
GTGTATAGAA AAGATCCCTA TCCTGAGGAA ATGATGAGAC AGAACCATGT TTTGAAACAG 
CCAGCTGTTG GTCACCCAGG GCAGAGGCCA GACAAAGAGC CTAATCTGAG CTATGAATCC 
CAACCCCCAT ATGTAGAAAA ACAGGCCAAC AGAGACCTCG AGCAGCCCAC ATACAGATAC 
GACTCCTCAA GCTATACAGA CCAGTTTTCT CGAAACTATG ATCATCGTCT CCGCTATGAA 
GAGCGCATTC CCACATATGA AGAGCAGTGG TCATATTACG ATGACAAACA GCCCTACCAG 
CCTCGGCCTT CTCTTGATAA TCAGCACCCT CGGGACCTTG ACTCCAGACA GCATCCAGAA 
GAGTCCTCAG AAAGAGGGTC TTACCCACGT TTTGAAGAGC CAGCCCCTCT GTCCTATGAC 
AGCAGACCAC GCTATGACCA GCCACCTAGA ACCTCTACCC TACGACACGA AGAGCAACCA 
ACTCCTGGAT ATGACATGCA TAACAGATAC AGACCAGAAG CGCAGTCCTA TTCTTCAGCA 
GGCCCTAAGG CATCTGAACC GAAGCAGTAT TTTGACCAGT ACCCACGAAG TTACGAGCAA 
GTACCATCAC AGGGATTTTC CTCGAAAGCC GGCCACTATG AGCCTCTCCA TGGTGCTGCG 
GTTGTCCCTC CTCTAATACC CGCATCTCAA CATAAGCCAG AAGTTCTGCC TTCAAATACC 
AAACCTCTGC CTCCACCCCC AACTCTGACT GAAGAGGAGG AAGATCCAGC AATGAAACCC 
CAGTCTGTAC TCACTAGAGT AAAAATGTTT GAAAACAAAA GATCTGCATC ATTGGAGAAC 
AAGAAGGATG AAAACCACAC CGCTGGTTTT AAGCCTCCAG AGGTAGCTTC TAAACCTCCA 
GGTGCTCCCA TCATTGGTCC TAAACCCACT CCTCAGAATC AGTTCAGTGA ACATGACAAA 
ACACTGTACA GGATCCCAGA ACCTCAAAAA CCTCAGATGA AGCCACCCGA AGATATTGTT 
CGGTCTAATC ATTATGATCC CGAAGAGGAT GAAGAATATT ATCGAAAGCA GCTCTCCTAC 
TTTGACCGAA GAAGTTTTGA AAACAAGCCT TCTACACACA TTCCTGCTGG CCATCTCTCA 
GAGCCTGCCA AGCCAGTTCA TTCTCAGAAT CAAACAAACT TTTCTAGTTA TTCTTCGAAG 
GGAAAGTCTC CTGAAGCTGA TGCCCCTGAT AGATCATTTG GTGAGAAGCG CTATGAGCCA 
GTCCAGGCCA CTCCCCCTCC TCCCCCATTG CCCTCCCAGT ATGCCCAGCC TTCTCAGCCC 
GGTACCAGCT CCTCTCTTGC CCTCCACACG CATGCCAAGG GGGCACATGG TGAAGGTAAT 
TCAATATCAC TGGACTTTCA GAATTCTTTA GTGTCCAAAC CAGACCCACC TCCATCACAG 
AATAAGCCAG CAACTTTCAG ACCACCAAAC CGAGAAGATA CTGTTCAGTC TACTTTCTAT 
CCACAGAAAA GTTTCCCAGA TAAAGCTCCA GTTAATGGAG CTGAACAGAC TCAGAAAACG 
GTCACTCCAG CATATAATCG ATTCACACCA AAACCATACA CAAGTTCTGC CCGGCCATTT 
GAACGCAAGT TTGAAAGTCC TAAATTCAAC CACAATCTCC TGCCAAGTGA AACAGCACAT 
AAACCTGACT TGTCTTCAAA AGCCCCTGCT TCTCCCAAGA CTCTTGCAAA AGCACATAGT 
CGAGCACAGC CTCCTGAGTT TGACAGTGGA GTTGAAACTT TCTCCATCCA TGCAGATAAA 
CCTAAATATC AAATGAACAA TCTCAGCACA GTGCCTAAAG CTATTCCTGT GAGCCCGTCA 
GCTGTGGAAG AAGATGAAGA TGAGGATGGC CATACTGTGG TGGCCACAGC CCGAGGCGTC 
TTTAACAACA ATGGTGGGGT GTTGAGTTCC ATAGAAACTG GCGTCAGTAT CATCATCCCC 
CAAGGAGCCA TTCCGGAGGG AGTCGAACAG GAAATCTACT TCAAGGTCTG CCGAGACAAC 
AGCATCCTCC CCCCTTTAGA TAAGGAGAAA GGTGAGACAC TGCTGAGCCC CTTAGTGATG 
TGTGGGCCCC ATGGCCTCAA GTTCCTGAAG CCCGTGGAGC TGCGCTTGCC ACACTGTGCG 
TCCATGACTC CTGACGGTTG GTCTTTTGCT CTAAAATCAT CCGACTCCTC GTCGGGTGAT 
CCTAAAACCT GGCAAAACAA GTGTCTTCCT GGAGATCCAA ATTACCTTGT TGGAGCAAAC 
TGTGTTTCTG TCCTGATTGA CCACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003140.1
CDS	13..5322
Translation	MSARAAAAKNTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSENEDSYDEEVHDPRSSRGGLVSRRSEKSWARDRSASRERSLSPRSDRRSVASSQPPKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSSGSLRSREEERISKPGAVSTPVKHADDHTHKTVEEVVVERNEKQAPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKTASQQKAEASSPVPYLSPETNPASSTSAVNHNVTLTNVRLEGPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLPSHVEPAKVYRKDPYPEEMMRQNHVLKQPAVGHPGQRPDKEPNLSYESQPPYVEKQANRDLEQPTYRYDSSSYTDQFSRNYDHRLRYEERIPTYEEQWSYYDDKQPYQPRPSLDNQHPRDLDSRQHPEESSERGSYPRFEEPAPLSYDSRPRYDQPPRTSTLRHEEQPTPGYDMHNRYRPEAQSYSSAGPKASEPKQYFDQYPRSYEQVPSQGFSSKAGHYEPLHGAAVVPPLIPASQHKPEVLPSNTKPLPPPPTLTEEEEDPAMKPQSVLTRVKMFENKRSASLENKKDENHTAGFKPPEVASKPPGAPIIGPKPTPQNQFSEHDKTLYRIPEPQKPQMKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPSTHIPAGHLSEPAKPVHSQNQTNFSSYSSKGKSPEADAPDRSFGEKRYEPVQATPPPPPLPSQYAQPSQPGTSSSLALHTHAKGAHGEGNSISLDFQNSLVSKPDPPPSQNKPATFRPPNREDTVQSTFYPQKSFPDKAPVNGAEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKAPASPKTLAKAHSRAQPPEFDSGVETFSIHADKPKYQMNNLSTVPKAIPVSPSAVEEDEDEDGHTVVATARGVFNNNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	NM_001003140.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris tight junction protein 1 (TJP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003140.1

ORF Insert Sequence:

ATGTCCGCCA GAGCTGCGGC CGCCAAGAAC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACACACAG TGACGCTTCA CAGGGCACCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGAAGAGATA ACCCTCATTT TCAGAGTGGA GAAACTTCCA TAGTAATTTC AGATGTGCTG 
AAAGGGGGAC CTGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAATGGA 
GTTTCAATGG ATAATGTTGA ACATGCGTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAG 
AATGCAAAAA TTACCATTCG GAGAAAGAAG AAAGTTCAGA TACCAGTTAG TCGTCCTGAT 
CCTGAACCAG TGTCTGAGAA TGAAGATAGT TATGATGAGG AAGTCCATGA CCCAAGAAGC 
AGCCGTGGTG GTCTGGTCAG TAGAAGGAGT GAGAAGAGTT GGGCAAGGGA CAGGAGTGCA 
AGCAGAGAGA GGAGTTTGTC TCCACGGTCT GATAGGCGAT CTGTGGCCTC CAGTCAACCT 
CCCAAACCTA CCAAAGTCAC ATTGGTGAAA TCCCGGAAAA ATGAAGAATA TGGTCTTCGA 
TTGGCCAGCC ATATATTTGT AAAGGAAATT TCACAAGATA GTTTGGCAGC AAGAGATGGC 
AATATTCAAG AAGGCGATGT TGTATTAAAG ATAAATGGTA CTGTGACAGA AAATATGTCA 
CTGACAGATG CAAAGACTTT GATAGAAAGA TCTAAAGGCA AGTTAAAAAT GGTAGTTCAA 
AGGGATGAAC GGGCTACCCT ATTGAATGTC CCTGATCTTT CTGACAGTAT CCACTCTGCT 
AATGCTTCTG AGAGAGATGA TATTTCAGAA ATTCAGTCAC TGGCATCAGA TCATTCTGGT 
CGATCACATG ATAGACCTCC CCGCCACAGC CGGTCACGGT CACCTGACCA ACGATCAGAG 
CCTTCTGATC ATTCCAGACA CTCTCCACAG CAGCCCAGCA GTGGCAGTCT CCGGAGCAGA 
GAGGAAGAGA GAATTTCTAA ACCTGGGGCT GTCTCAACTC CTGTAAAGCA TGCAGATGAT 
CATACACATA AAACAGTAGA AGAAGTTGTA GTTGAGAGAA ATGAGAAACA AGCACCCAGT 
CTTCCAGAAC CAAAGCCTGT GTATGCTCAA GTTGGACAAC CAGATGTGGA TTTACCTGTC 
AGTCCATCTG ATGGCGTCCT ACCTAATTCA ACTCATGAAG ATGGGATTCT CCGGCCCAGC 
ATGAAATTGG TAAAATTCAG AAAAGGAGAT AGTGTGGGCC TGCGCCTGGC TGGTGGAAAT 
GATGTTGGAA TATTTGTAGC TGGTGTTCTA GAAGATAGCC CTGCAGCCAA AGAAGGCTTA 
GAAGAGGGTG ATCAAATTCT CAGGGTAAAC AATGTAGATT TCACAAACAT CATAAGAGAA 
GAAGCCGTCC TTTTCCTACT CGACCTCCCT AAAGGAGAAG AAGTGACCAT ATTGGCTCAG 
AAGAAGAAAG ATGTTTATCG TCGCATTGTA GAATCAGATG TGGGAGATTC TTTCTATATT 
AGAACCCACT TTGAATATGA AAAGGAATCT CCCTACGGAC TTAGTTTTAA CAAAGGAGAG 
GTGTTTCGTG TTGTGGATAC CTTGTACAAT GGGAAACTAG GCTCTTGGCT CGCTATTCGA 
ATTGGCAAAA ATCATAAAGA AGTAGAACGA GGCATCATCC CTAATAAGAA CAGGGCTGAG 
CAGTTAGCCA GTGTACAGTA CACACTTCCA AAAACAGCTG GGGGAGACCG TGCTGACTTC 
TGGAGATTCC GGGGTCTTCG CAGCTCCAAG AGAAATCTTC GAAAGAGTAG AGAGGATTTG 
TCAGCTCAGC CAGTTCAAAC AAAGTTTCCA GCCTATGAAA GAGTTGTTCT TCGAGAAGCT 
GGATTTCTGA GGCCTGTAAC TATCTTTGGA CCCATAGCTG ATGTTGCCAG AGAAAAGCTG 
GCAAGAGAAG AACCAGATAT TTATCAGATT GCAAAAAGTG AACCACGAGA TGCTGGAACT 
GATCAACGTA GCTCTGGTAT TATTCGTCTT CATACAATAA AACAAATAAT AGATCAGGAC 
AAACATGCTT TATTAGATGT AACACCAAAT GCAGTTGATC GTCTGAATTA TGCCCAGTGG 
TATCCAATTG TAGTATTCCT TAACCCTGAT TCTAAGCAAG GTGTGAAAAC TATGAGGATG 
AGGCTATGCC CAGAATCTCG AAAAAGTGCC AGGAAATTAT ATGAACGGTC TCATAAACTT 
CGTAAAAATA ATCACCATCT TTTTACAACT ACAATTAACT TAAATTCAAT GAATGATGGT 
TGGTATGGTG CATTGAAAGA AGCAATTCAG CAGCAACAGA ACCAGTTGGT ATGGGTTTCT 
GAGGGAAAGG CGGATGGTGC TACAAGTGAT GACCTTGATT TGCATGATGA TCGTCTCTCC 
TACCTGTCAG CCCCAGGTAG TGAATACTCA ATGTATAGCA CGGACAGTAG ACACACTTCT 
GACTATGAAG ACACAGATAC AGAAGGCGGG GCCTACACTG ATCAAGAACT AGATGAAACT 
CTTAATGATG AGGTTGGGAC TCCACCGGAG TCTGCCATTA CACGGTCCTC TGAGCCTGTA 
AGAGAGGACT CCTCTGGAAT GCATCATGAA AACCAAACAT ATCCTCCTTA TTCACCACAA 
GCGCAGCCAC AACCAATTCA TAGAATAGAC TCCCCTGGAT TTAAAACAGC TTCTCAACAG 
AAAGCAGAAG CCTCATCTCC AGTCCCTTAC CTTTCGCCTG AAACAAACCC AGCATCATCA 
ACCTCTGCTG TTAACCATAA TGTAACTTTA ACTAATGTCA GACTGGAGGG GCCTACCCCA 
GCTCCTTCTA CTTCTTACTC ACCACAAGCT GATTCTTTAA GAACACCAAG CACTGAGGCA 
GCTCACATAA TGCTAAGAGA TCAAGAGCCA TCATTGCCGT CGCATGTAGA GCCAGCAAAG 
GTGTATAGAA AAGATCCCTA TCCTGAGGAA ATGATGAGAC AGAACCATGT TTTGAAACAG 
CCAGCTGTTG GTCACCCAGG GCAGAGGCCA GACAAAGAGC CTAATCTGAG CTATGAATCC 
CAACCCCCAT ATGTAGAAAA ACAGGCCAAC AGAGACCTCG AGCAGCCCAC ATACAGATAC 
GACTCCTCAA GCTATACAGA CCAGTTTTCT CGAAACTATG ATCATCGTCT CCGCTATGAA 
GAGCGCATTC CCACATATGA AGAGCAGTGG TCATATTACG ATGACAAACA GCCCTACCAG 
CCTCGGCCTT CTCTTGATAA TCAGCACCCT CGGGACCTTG ACTCCAGACA GCATCCAGAA 
GAGTCCTCAG AAAGAGGGTC TTACCCACGT TTTGAAGAGC CAGCCCCTCT GTCCTATGAC 
AGCAGACCAC GCTATGACCA GCCACCTAGA ACCTCTACCC TACGACACGA AGAGCAACCA 
ACTCCTGGAT ATGACATGCA TAACAGATAC AGACCAGAAG CGCAGTCCTA TTCTTCAGCA 
GGCCCTAAGG CATCTGAACC GAAGCAGTAT TTTGACCAGT ACCCACGAAG TTACGAGCAA 
GTACCATCAC AGGGATTTTC CTCGAAAGCC GGCCACTATG AGCCTCTCCA TGGTGCTGCG 
GTTGTCCCTC CTCTAATACC CGCATCTCAA CATAAGCCAG AAGTTCTGCC TTCAAATACC 
AAACCTCTGC CTCCACCCCC AACTCTGACT GAAGAGGAGG AAGATCCAGC AATGAAACCC 
CAGTCTGTAC TCACTAGAGT AAAAATGTTT GAAAACAAAA GATCTGCATC ATTGGAGAAC 
AAGAAGGATG AAAACCACAC CGCTGGTTTT AAGCCTCCAG AGGTAGCTTC TAAACCTCCA 
GGTGCTCCCA TCATTGGTCC TAAACCCACT CCTCAGAATC AGTTCAGTGA ACATGACAAA 
ACACTGTACA GGATCCCAGA ACCTCAAAAA CCTCAGATGA AGCCACCCGA AGATATTGTT 
CGGTCTAATC ATTATGATCC CGAAGAGGAT GAAGAATATT ATCGAAAGCA GCTCTCCTAC 
TTTGACCGAA GAAGTTTTGA AAACAAGCCT TCTACACACA TTCCTGCTGG CCATCTCTCA 
GAGCCTGCCA AGCCAGTTCA TTCTCAGAAT CAAACAAACT TTTCTAGTTA TTCTTCGAAG 
GGAAAGTCTC CTGAAGCTGA TGCCCCTGAT AGATCATTTG GTGAGAAGCG CTATGAGCCA 
GTCCAGGCCA CTCCCCCTCC TCCCCCATTG CCCTCCCAGT ATGCCCAGCC TTCTCAGCCC 
GGTACCAGCT CCTCTCTTGC CCTCCACACG CATGCCAAGG GGGCACATGG TGAAGGTAAT 
TCAATATCAC TGGACTTTCA GAATTCTTTA GTGTCCAAAC CAGACCCACC TCCATCACAG 
AATAAGCCAG CAACTTTCAG ACCACCAAAC CGAGAAGATA CTGTTCAGTC TACTTTCTAT 
CCACAGAAAA GTTTCCCAGA TAAAGCTCCA GTTAATGGAG CTGAACAGAC TCAGAAAACG 
GTCACTCCAG CATATAATCG ATTCACACCA AAACCATACA CAAGTTCTGC CCGGCCATTT 
GAACGCAAGT TTGAAAGTCC TAAATTCAAC CACAATCTCC TGCCAAGTGA AACAGCACAT 
AAACCTGACT TGTCTTCAAA AGCCCCTGCT TCTCCCAAGA CTCTTGCAAA AGCACATAGT 
CGAGCACAGC CTCCTGAGTT TGACAGTGGA GTTGAAACTT TCTCCATCCA TGCAGATAAA 
CCTAAATATC AAATGAACAA TCTCAGCACA GTGCCTAAAG CTATTCCTGT GAGCCCGTCA 
GCTGTGGAAG AAGATGAAGA TGAGGATGGC CATACTGTGG TGGCCACAGC CCGAGGCGTC 
TTTAACAACA ATGGTGGGGT GTTGAGTTCC ATAGAAACTG GCGTCAGTAT CATCATCCCC 
CAAGGAGCCA TTCCGGAGGG AGTCGAACAG GAAATCTACT TCAAGGTCTG CCGAGACAAC 
AGCATCCTCC CCCCTTTAGA TAAGGAGAAA GGTGAGACAC TGCTGAGCCC CTTAGTGATG 
TGTGGGCCCC ATGGCCTCAA GTTCCTGAAG CCCGTGGAGC TGCGCTTGCC ACACTGTGCG 
TCCATGACTC CTGACGGTTG GTCTTTTGCT CTAAAATCAT CCGACTCCTC GTCGGGTGAT 
CCTAAAACCT GGCAAAACAA GTGTCTTCCT GGAGATCCAA ATTACCTTGT TGGAGCAAAC 
TGTGTTTCTG TCCTGATTGA CCACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression of claudins, occludin, junction adhesion molecule A and zona occludens 1 in canine organs.
Molecular medicine reports14(4)3697-703(2016 Oct)
Ahn C,Shin DH,Lee D,Kang SM,Seok JH,Kang HY,Jeung EB

Tight junction proteins in the canine epidermis: a pilot study on their distribution in normal and in high IgE-producing canines.
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire79(1)46-51(2015 Jan)
Roussel AJ,Bruet V,Marsella R,Knol AC,Bourdeau PJ

ZO-1 knockout by TALEN-mediated gene targeting in MDCK cells: involvement of ZO-1 in the regulation of cytoskeleton and cell shape.
PloS one9(8)e104994(2014)
Tokuda S,Higashi T,Furuse M

Injury-triggered Akt phosphorylation of Cx43: a ZO-1-driven molecular switch that regulates gap junction size.
Journal of cell science127(Pt 2)455-64(2014 Jan)
Dunn CA,Lampe PD

ZO-1 recruitment to ?-catenin--a novel mechanism for coupling the assembly of tight junctions to adherens junctions.
Journal of cell science126(Pt 17)3904-15(2013 Sep)
Maiers JL,Peng X,Fanning AS,DeMali KA

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TJP1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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