LOC100856005 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100856005
Entrez Gene ID	100856005
Full Name	rho guanine nucleotide exchange factor 1-like
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014112162.2	XP_013967637.1	rho guanine nucleotide exchange factor 1-like isoform X2
XM_014112163.2	XP_013967638.1	rho guanine nucleotide exchange factor 1-like isoform X3
XM_014112161.1	XP_013967636.1	rho guanine nucleotide exchange factor 1-like isoform X2
XM_014112162.1	XP_013967637.1	rho guanine nucleotide exchange factor 1-like isoform X3
XM_003640309.4	XP_003640357.3	rho guanine nucleotide exchange factor 1-like isoform X1
XM_003640309.3	XP_003640357.3	rho guanine nucleotide exchange factor 1-like isoform X1
XM_014112163.1	XP_013967638.1	rho guanine nucleotide exchange factor 1-like isoform X4

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	arhgef1	XP_002941974.2
Canis lupus familiaris (dog)	ARHGEF1	XP_005616572.1
Canis lupus familiaris (dog)	LOC100856005	XP_003640357.2
Pan troglodytes (chimpanzee)	ARHGEF1	XP_003953551.1
Mus musculus (house mouse)	Arhgef1	NP_032514.1
Rattus norvegicus (Norway rat)	Arhgef1	NP_067726.3
Homo sapiens (human)	ARHGEF1	NP_945353.1
Bos taurus (cattle)	ARHGEF1	XP_005219451.1

Gene Ontology

The following LOC100856005 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100856005 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe45888	XM_003640309.4 Latest version!	Canis lupus familiaris rho guanine nucleotide exchange factor 1-like (LOC100856005), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OCe45889	XM_014112161.1 Latest version!	Canis lupus familiaris rho guanine nucleotide exchange factor 1-like (LOC100856005), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OCe45890	XM_014112162.2 Latest version!	Canis lupus familiaris rho guanine nucleotide exchange factor 1-like (LOC100856005), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OCe45891	XM_014112163.2 Latest version!	Canis lupus familiaris rho guanine nucleotide exchange factor 1-like (LOC100856005), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
View Old Accession Versions >>
OCe45890	XM_014112162.1	Canis lupus familiaris rho guanine nucleotide exchange factor 1-like (LOC100856005), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OCe45888	XM_003640309.3	Canis lupus familiaris rho guanine nucleotide exchange factor 1-like (LOC100856005), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OCe45891	XM_014112163.1	Canis lupus familiaris rho guanine nucleotide exchange factor 1-like (LOC100856005), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100856005 ( XM_003640309.4 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_003640357.3 Canis lupus familiaris rho guanine nucleotide exchange factor 1-like (LOC100856005), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe45888
Clone ID Related Accession (Same CDS sequence)	XM_003640309.4 , XM_003640309.3
Accession Version	XM_003640309.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	rho guanine nucleotide exchange factor 1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003727276.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003640309.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGTT CCCTGGGCCC CAAGGAGGCC AAGAAGGCCT TCCTCGACTT CTGCCATTGC 
TTCCTGGACA AGACGGCGGT TTTGCGGGTG CTAGTCCCTC CCGCCGTCGC CTTTGAACTT 
GACCGCACGC GGCCCGAGGT CATCTCCGAG GACTTGCAGC GGCAGTTTGT ACAGGAGGTA 
GTGCAGAGCC AGCAGGCAGC TGTTAGCCGG CTGCTGGAGG ACTTCCGCTC CAAGCGGCTC 
ATGGGCATGA CACCCTGGGA GCAGGACCTG ACCCAGCTGG AGGCCTGGGT TGGGCGGGAC 
CGTGCCAGCT TTGAGGCCCG GGAGCGGCAC CTGGCTGAAC GGCTGCTGGC CCACCTGGAG 
GAGATGCAAC ACACCATCTC TAACGATGAG GAAAAGAGTG CCGCTGTGGT CAATGCCATC 
AGCCTGTACA TGCGCCACCT TGGGGTGCGG ACCAAGAGCG GAGACAAGAA GTCAGGGATG 
AATTTCTTCC GCAAAAAGGT GATGGGGAAT CGGCGGTCAG ATGAGCCAAC CAAGACCAAA 
AAAGGCCTGA GCATCTTCCT AGACCCTGCT CGCTGGAACC GGGGAGAGCC TCAGGCCTCG 
GATTTCCGAC ACCTCAAAGT CGAGACTGAT GCTGAGAAAC CAGGCCTTAC GGAAAGGAAG 
GGAGGCCTGG GGGTGCCCTC CCGGGACCGG AATGTTGGGG CTCCTGGGCA GGATGTCCCT 
GGAGTTTCTC TGCACCCTCT GCCCGGGGAC AGCCCTGACC GGGAACCAGG TGTTGACACC 
CCGCCAGAGC TGGGGGACCC ACCCCCCCAG GGCCCGGCCA GCCTGGAGCC CCCGGGGCCC 
TCGGAGAGCA CTGAGGAGGG TCCTGACACA GAGAGAAGGT GGAAGAGGCT GTCGGGGCGT 
CTGGGGCGCT CGGAGAGCCT GCGGGTGAGT GACCGCCGCC GGCCTTCCCG GGGCAGCCTC 
GGGGCTAAGG GCCGGGGTGG GGGCCGCTCC CGGAGTGACG TGGACATGGA CCCCAGCTCT 
GCCACGGCCG TGCTTGGCCC TGCCCGACGA GCCACCCCTG AGCCTGGAGA CGAAGGGGAC 
CCTGGGCGGT CAGGACTAGA GCTGGAACCA GAAGAGCCCC CTGGCTGGCG GGAGCTCATC 
CCCCCAGGCA CCCTGCACAG CCTGCCTAAG AGCCAGGTGA AGCGGCAGGA GGTCATCAGC 
GAGCTACTGG TGACAGAGGC TGCCCATGTG CGCATGCTCC GGGTGCTGCA TGACCTCTTC 
TACCAGCCCA TGTTGGAAGG GAACTTCTTC TCCACGGAGG ATCTACAGAA CATCTTCCCC 
AGCCTGGATG AGCTCATCGA GGTGCATTCC CTGTTCCTCG ATCGACTGAT GAAGCGGAGG 
CAGGATAGTG GCTGCCTCAT TGAGGAGATC GGAGATGTAC TGCTGGCCCG GTTTGATGGT 
GCCGAAGGCA AATGGTTCCA GAAAATCTCC TCCCGCTTCT GCAGCCGCCA GTCATTTGCC 
TTAGAGCAGC TCAAAGCCAA GCAGCGCAAG GATCCTCGGT TCTGTGCCTT TGTGCAGGAG 
GCCGAGAGCC GCCCCCGGTG CCGCCGCCTG CAGCTGAAGG ATATGATCCC CACGGAGATG 
CAGCGTCTGA CCAAGTACCC CCTGCTCCTG CAGAGCATTG GGCAGAACAC AGAAGAGTCA 
GCAGAACGGG AGAAAGTAGA ACTGGCAGCT GAGTGCTGCC GGGAAATTCT GCACCATGTT 
AACCAAGCCG TGCGTGACAT GGAGGACCTG CTGCGGCTCA AGGATTATCA GAAGCGCCTG 
GACTTGTCCC TCCTGCGGCA GAGCAGCGAC CCCATGCTAA GCGAGTTCAA GAACCTGGAC 
ATCACCAAAA AGAAGCTGAT CTACGAGGGT CCGCTGACGT GGCGGCTGAC GAAGGACAAG 
GCTGTAGAGG TACACGTGCT GCTGCTGGAT GACCTGCTGC TGCTGCTCCA GCGCCAGGAC 
GATCGGCTGC TGCTCAAGTC CCACAGCCGG ACGCTGACAC CCACACCCGA CGGCAAGACC 
ATGCTGCGGC CGGTGCTGCG GCTCACCTCC GCCATAACCC GCGAGGTGGC TACCGATCAC 
AAAGCCTTCT ATGTCCTTTT TACCTGGGAC CAGGAGGCCC AGATATATGA GCTGGTGGCC 
CAGACGGTGT CGGAACGGAA GAACTGGTGT TCCCTCATCA CTGAAACTGC TGGATCCTTG 
AAGGTCCCTG CCCCCGCCTC TCGCCCCAAA CCCCGGCCCA GCCCAAGCAG CACCCGAGAA 
CCCCTGCTCA GCAGCTCAGA GAATGGCAAC GGTGGTGGCC GAGAGATGCC CCCAGCTGAC 
GGCCAGACGG AGAGAATCCT CAGCACCCTC CTGCCCTACT GCAGGCCCCG CCCTGAGGGC 
CAGCTTGCTG CCAAGGCCCT TGAGAAAGTG CTGTCCCTGA AGCAGCTCCT GTTTCCCTCG 
GAGGAAGACA GTGGGGCAGG GCCTCCCCAG GAAGGGGATG GGGTCCCTGG GGGTGGCCCC 
CTGAACCCGA CACAGACCCA CACCCAGGAA ATTCGGGAAA AGCTGCTCAG CCTGGAGGAG 
ACCATGAAAC AGCTGGAGGA GGTGGAGGAG GAATTTTGTC ACCTGCGACT CCTCCTGTCT 
CAGCTTGGGG AGAACAGCAC CCCCCAGCCT GGGTGTACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003640357.3
CDS	482..3163
Translation	MLSSLGPKEAKKAFLDFCHCFLDKTAVLRVLVPPAVAFELDRTRPEVISEDLQRQFVQEVVQSQQAAVSRLLEDFRSKRLMGMTPWEQDLTQLEAWVGRDRASFEARERHLAERLLAHLEEMQHTISNDEEKSAAVVNAISLYMRHLGVRTKSGDKKSGMNFFRKKVMGNRRSDEPTKTKKGLSIFLDPARWNRGEPQASDFRHLKVETDAEKPGLTERKGGLGVPSRDRNVGAPGQDVPGVSLHPLPGDSPDREPGVDTPPELGDPPPQGPASLEPPGPSESTEEGPDTERRWKRLSGRLGRSESLRVSDRRRPSRGSLGAKGRGGGRSRSDVDMDPSSATAVLGPARRATPEPGDEGDPGRSGLELEPEEPPGWRELIPPGTLHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDLFYQPMLEGNFFSTEDLQNIFPSLDELIEVHSLFLDRLMKRRQDSGCLIEEIGDVLLARFDGAEGKWFQKISSRFCSRQSFALEQLKAKQRKDPRFCAFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIGQNTEESAEREKVELAAECCREILHHVNQAVRDMEDLLRLKDYQKRLDLSLLRQSSDPMLSEFKNLDITKKKLIYEGPLTWRLTKDKAVEVHVLLLDDLLLLLQRQDDRLLLKSHSRTLTPTPDGKTMLRPVLRLTSAITREVATDHKAFYVLFTWDQEAQIYELVAQTVSERKNWCSLITETAGSLKVPAPASRPKPRPSPSSTREPLLSSSENGNGGGREMPPADGQTERILSTLLPYCRPRPEGQLAAKALEKVLSLKQLLFPSEEDSGAGPPQEGDGVPGGGPLNPTQTHTQEIREKLLSLEETMKQLEEVEEEFCHLRLLLSQLGENSTPQPGCT

Target ORF information:

RefSeq Version	XM_003640309.4
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris rho guanine nucleotide exchange factor 1-like (LOC100856005), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003640309.4

ORF Insert Sequence:

ATGCTGAGTT CCCTGGGCCC CAAGGAGGCC AAGAAGGCCT TCCTCGACTT CTGCCATTGC 
TTCCTGGACA AGACGGCGGT TTTGCGGGTG CTAGTCCCTC CCGCCGTCGC CTTTGAACTT 
GACCGCACGC GGCCCGAGGT CATCTCCGAG GACTTGCAGC GGCAGTTTGT ACAGGAGGTA 
GTGCAGAGCC AGCAGGCAGC TGTTAGCCGG CTGCTGGAGG ACTTCCGCTC CAAGCGGCTC 
ATGGGCATGA CACCCTGGGA GCAGGACCTG ACCCAGCTGG AGGCCTGGGT TGGGCGGGAC 
CGTGCCAGCT TTGAGGCCCG GGAGCGGCAC CTGGCTGAAC GGCTGCTGGC CCACCTGGAG 
GAGATGCAAC ACACCATCTC TAACGATGAG GAAAAGAGTG CCGCTGTGGT CAATGCCATC 
AGCCTGTACA TGCGCCACCT TGGGGTGCGG ACCAAGAGCG GAGACAAGAA GTCAGGGATG 
AATTTCTTCC GCAAAAAGGT GATGGGGAAT CGGCGGTCAG ATGAGCCAAC CAAGACCAAA 
AAAGGCCTGA GCATCTTCCT AGACCCTGCT CGCTGGAACC GGGGAGAGCC TCAGGCCTCG 
GATTTCCGAC ACCTCAAAGT CGAGACTGAT GCTGAGAAAC CAGGCCTTAC GGAAAGGAAG 
GGAGGCCTGG GGGTGCCCTC CCGGGACCGG AATGTTGGGG CTCCTGGGCA GGATGTCCCT 
GGAGTTTCTC TGCACCCTCT GCCCGGGGAC AGCCCTGACC GGGAACCAGG TGTTGACACC 
CCGCCAGAGC TGGGGGACCC ACCCCCCCAG GGCCCGGCCA GCCTGGAGCC CCCGGGGCCC 
TCGGAGAGCA CTGAGGAGGG TCCTGACACA GAGAGAAGGT GGAAGAGGCT GTCGGGGCGT 
CTGGGGCGCT CGGAGAGCCT GCGGGTGAGT GACCGCCGCC GGCCTTCCCG GGGCAGCCTC 
GGGGCTAAGG GCCGGGGTGG GGGCCGCTCC CGGAGTGACG TGGACATGGA CCCCAGCTCT 
GCCACGGCCG TGCTTGGCCC TGCCCGACGA GCCACCCCTG AGCCTGGAGA CGAAGGGGAC 
CCTGGGCGGT CAGGACTAGA GCTGGAACCA GAAGAGCCCC CTGGCTGGCG GGAGCTCATC 
CCCCCAGGCA CCCTGCACAG CCTGCCTAAG AGCCAGGTGA AGCGGCAGGA GGTCATCAGC 
GAGCTACTGG TGACAGAGGC TGCCCATGTG CGCATGCTCC GGGTGCTGCA TGACCTCTTC 
TACCAGCCCA TGTTGGAAGG GAACTTCTTC TCCACGGAGG ATCTACAGAA CATCTTCCCC 
AGCCTGGATG AGCTCATCGA GGTGCATTCC CTGTTCCTCG ATCGACTGAT GAAGCGGAGG 
CAGGATAGTG GCTGCCTCAT TGAGGAGATC GGAGATGTAC TGCTGGCCCG GTTTGATGGT 
GCCGAAGGCA AATGGTTCCA GAAAATCTCC TCCCGCTTCT GCAGCCGCCA GTCATTTGCC 
TTAGAGCAGC TCAAAGCCAA GCAGCGCAAG GATCCTCGGT TCTGTGCCTT TGTGCAGGAG 
GCCGAGAGCC GCCCCCGGTG CCGCCGCCTG CAGCTGAAGG ATATGATCCC CACGGAGATG 
CAGCGTCTGA CCAAGTACCC CCTGCTCCTG CAGAGCATTG GGCAGAACAC AGAAGAGTCA 
GCAGAACGGG AGAAAGTAGA ACTGGCAGCT GAGTGCTGCC GGGAAATTCT GCACCATGTT 
AACCAAGCCG TGCGTGACAT GGAGGACCTG CTGCGGCTCA AGGATTATCA GAAGCGCCTG 
GACTTGTCCC TCCTGCGGCA GAGCAGCGAC CCCATGCTAA GCGAGTTCAA GAACCTGGAC 
ATCACCAAAA AGAAGCTGAT CTACGAGGGT CCGCTGACGT GGCGGCTGAC GAAGGACAAG 
GCTGTAGAGG TACACGTGCT GCTGCTGGAT GACCTGCTGC TGCTGCTCCA GCGCCAGGAC 
GATCGGCTGC TGCTCAAGTC CCACAGCCGG ACGCTGACAC CCACACCCGA CGGCAAGACC 
ATGCTGCGGC CGGTGCTGCG GCTCACCTCC GCCATAACCC GCGAGGTGGC TACCGATCAC 
AAAGCCTTCT ATGTCCTTTT TACCTGGGAC CAGGAGGCCC AGATATATGA GCTGGTGGCC 
CAGACGGTGT CGGAACGGAA GAACTGGTGT TCCCTCATCA CTGAAACTGC TGGATCCTTG 
AAGGTCCCTG CCCCCGCCTC TCGCCCCAAA CCCCGGCCCA GCCCAAGCAG CACCCGAGAA 
CCCCTGCTCA GCAGCTCAGA GAATGGCAAC GGTGGTGGCC GAGAGATGCC CCCAGCTGAC 
GGCCAGACGG AGAGAATCCT CAGCACCCTC CTGCCCTACT GCAGGCCCCG CCCTGAGGGC 
CAGCTTGCTG CCAAGGCCCT TGAGAAAGTG CTGTCCCTGA AGCAGCTCCT GTTTCCCTCG 
GAGGAAGACA GTGGGGCAGG GCCTCCCCAG GAAGGGGATG GGGTCCCTGG GGGTGGCCCC 
CTGAACCCGA CACAGACCCA CACCCAGGAA ATTCGGGAAA AGCTGCTCAG CCTGGAGGAG 
ACCATGAAAC AGCTGGAGGA GGTGGAGGAG GAATTTTGTC ACCTGCGACT CCTCCTGTCT 
CAGCTTGGGG AGAACAGCAC CCCCCAGCCT GGGTGTACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100856005 ( XM_003640309.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_003640357.3 Canis lupus familiaris rho guanine nucleotide exchange factor 1-like (LOC100856005), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe45888
Clone ID Related Accession (Same CDS sequence)	XM_003640309.4 , XM_003640309.3
Accession Version	XM_003640309.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	rho guanine nucleotide exchange factor 1-like isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003727276.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003640309.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGTT CCCTGGGCCC CAAGGAGGCC AAGAAGGCCT TCCTCGACTT CTGCCATTGC 
TTCCTGGACA AGACGGCGGT TTTGCGGGTG CTAGTCCCTC CCGCCGTCGC CTTTGAACTT 
GACCGCACGC GGCCCGAGGT CATCTCCGAG GACTTGCAGC GGCAGTTTGT ACAGGAGGTA 
GTGCAGAGCC AGCAGGCAGC TGTTAGCCGG CTGCTGGAGG ACTTCCGCTC CAAGCGGCTC 
ATGGGCATGA CACCCTGGGA GCAGGACCTG ACCCAGCTGG AGGCCTGGGT TGGGCGGGAC 
CGTGCCAGCT TTGAGGCCCG GGAGCGGCAC CTGGCTGAAC GGCTGCTGGC CCACCTGGAG 
GAGATGCAAC ACACCATCTC TAACGATGAG GAAAAGAGTG CCGCTGTGGT CAATGCCATC 
AGCCTGTACA TGCGCCACCT TGGGGTGCGG ACCAAGAGCG GAGACAAGAA GTCAGGGATG 
AATTTCTTCC GCAAAAAGGT GATGGGGAAT CGGCGGTCAG ATGAGCCAAC CAAGACCAAA 
AAAGGCCTGA GCATCTTCCT AGACCCTGCT CGCTGGAACC GGGGAGAGCC TCAGGCCTCG 
GATTTCCGAC ACCTCAAAGT CGAGACTGAT GCTGAGAAAC CAGGCCTTAC GGAAAGGAAG 
GGAGGCCTGG GGGTGCCCTC CCGGGACCGG AATGTTGGGG CTCCTGGGCA GGATGTCCCT 
GGAGTTTCTC TGCACCCTCT GCCCGGGGAC AGCCCTGACC GGGAACCAGG TGTTGACACC 
CCGCCAGAGC TGGGGGACCC ACCCCCCCAG GGCCCGGCCA GCCTGGAGCC CCCGGGGCCC 
TCGGAGAGCA CTGAGGAGGG TCCTGACACA GAGAGAAGGT GGAAGAGGCT GTCGGGGCGT 
CTGGGGCGCT CGGAGAGCCT GCGGGTGAGT GACCGCCGCC GGCCTTCCCG GGGCAGCCTC 
GGGGCTAAGG GCCGGGGTGG GGGCCGCTCC CGGAGTGACG TGGACATGGA CCCCAGCTCT 
GCCACGGCCG TGCTTGGCCC TGCCCGACGA GCCACCCCTG AGCCTGGAGA CGAAGGGGAC 
CCTGGGCGGT CAGGACTAGA GCTGGAACCA GAAGAGCCCC CTGGCTGGCG GGAGCTCATC 
CCCCCAGGCA CCCTGCACAG CCTGCCTAAG AGCCAGGTGA AGCGGCAGGA GGTCATCAGC 
GAGCTACTGG TGACAGAGGC TGCCCATGTG CGCATGCTCC GGGTGCTGCA TGACCTCTTC 
TACCAGCCCA TGTTGGAAGG GAACTTCTTC TCCACGGAGG ATCTACAGAA CATCTTCCCC 
AGCCTGGATG AGCTCATCGA GGTGCATTCC CTGTTCCTCG ATCGACTGAT GAAGCGGAGG 
CAGGATAGTG GCTGCCTCAT TGAGGAGATC GGAGATGTAC TGCTGGCCCG GTTTGATGGT 
GCCGAAGGCA AATGGTTCCA GAAAATCTCC TCCCGCTTCT GCAGCCGCCA GTCATTTGCC 
TTAGAGCAGC TCAAAGCCAA GCAGCGCAAG GATCCTCGGT TCTGTGCCTT TGTGCAGGAG 
GCCGAGAGCC GCCCCCGGTG CCGCCGCCTG CAGCTGAAGG ATATGATCCC CACGGAGATG 
CAGCGTCTGA CCAAGTACCC CCTGCTCCTG CAGAGCATTG GGCAGAACAC AGAAGAGTCA 
GCAGAACGGG AGAAAGTAGA ACTGGCAGCT GAGTGCTGCC GGGAAATTCT GCACCATGTT 
AACCAAGCCG TGCGTGACAT GGAGGACCTG CTGCGGCTCA AGGATTATCA GAAGCGCCTG 
GACTTGTCCC TCCTGCGGCA GAGCAGCGAC CCCATGCTAA GCGAGTTCAA GAACCTGGAC 
ATCACCAAAA AGAAGCTGAT CTACGAGGGT CCGCTGACGT GGCGGCTGAC GAAGGACAAG 
GCTGTAGAGG TACACGTGCT GCTGCTGGAT GACCTGCTGC TGCTGCTCCA GCGCCAGGAC 
GATCGGCTGC TGCTCAAGTC CCACAGCCGG ACGCTGACAC CCACACCCGA CGGCAAGACC 
ATGCTGCGGC CGGTGCTGCG GCTCACCTCC GCCATAACCC GCGAGGTGGC TACCGATCAC 
AAAGCCTTCT ATGTCCTTTT TACCTGGGAC CAGGAGGCCC AGATATATGA GCTGGTGGCC 
CAGACGGTGT CGGAACGGAA GAACTGGTGT TCCCTCATCA CTGAAACTGC TGGATCCTTG 
AAGGTCCCTG CCCCCGCCTC TCGCCCCAAA CCCCGGCCCA GCCCAAGCAG CACCCGAGAA 
CCCCTGCTCA GCAGCTCAGA GAATGGCAAC GGTGGTGGCC GAGAGATGCC CCCAGCTGAC 
GGCCAGACGG AGAGAATCCT CAGCACCCTC CTGCCCTACT GCAGGCCCCG CCCTGAGGGC 
CAGCTTGCTG CCAAGGCCCT TGAGAAAGTG CTGTCCCTGA AGCAGCTCCT GTTTCCCTCG 
GAGGAAGACA GTGGGGCAGG GCCTCCCCAG GAAGGGGATG GGGTCCCTGG GGGTGGCCCC 
CTGAACCCGA CACAGACCCA CACCCAGGAA ATTCGGGAAA AGCTGCTCAG CCTGGAGGAG 
ACCATGAAAC AGCTGGAGGA GGTGGAGGAG GAATTTTGTC ACCTGCGACT CCTCCTGTCT 
CAGCTTGGGG AGAACAGCAC CCCCCAGCCT GGGTGTACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003640357.3
CDS	458..3139
Translation	MLSSLGPKEAKKAFLDFCHCFLDKTAVLRVLVPPAVAFELDRTRPEVISEDLQRQFVQEVVQSQQAAVSRLLEDFRSKRLMGMTPWEQDLTQLEAWVGRDRASFEARERHLAERLLAHLEEMQHTISNDEEKSAAVVNAISLYMRHLGVRTKSGDKKSGMNFFRKKVMGNRRSDEPTKTKKGLSIFLDPARWNRGEPQASDFRHLKVETDAEKPGLTERKGGLGVPSRDRNVGAPGQDVPGVSLHPLPGDSPDREPGVDTPPELGDPPPQGPASLEPPGPSESTEEGPDTERRWKRLSGRLGRSESLRVSDRRRPSRGSLGAKGRGGGRSRSDVDMDPSSATAVLGPARRATPEPGDEGDPGRSGLELEPEEPPGWRELIPPGTLHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDLFYQPMLEGNFFSTEDLQNIFPSLDELIEVHSLFLDRLMKRRQDSGCLIEEIGDVLLARFDGAEGKWFQKISSRFCSRQSFALEQLKAKQRKDPRFCAFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIGQNTEESAEREKVELAAECCREILHHVNQAVRDMEDLLRLKDYQKRLDLSLLRQSSDPMLSEFKNLDITKKKLIYEGPLTWRLTKDKAVEVHVLLLDDLLLLLQRQDDRLLLKSHSRTLTPTPDGKTMLRPVLRLTSAITREVATDHKAFYVLFTWDQEAQIYELVAQTVSERKNWCSLITETAGSLKVPAPASRPKPRPSPSSTREPLLSSSENGNGGGREMPPADGQTERILSTLLPYCRPRPEGQLAAKALEKVLSLKQLLFPSEEDSGAGPPQEGDGVPGGGPLNPTQTHTQEIREKLLSLEETMKQLEEVEEEFCHLRLLLSQLGENSTPQPGCT

Target ORF information:

RefSeq Version	XM_003640309.3
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris rho guanine nucleotide exchange factor 1-like (LOC100856005), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003640309.3

ORF Insert Sequence:

ATGCTGAGTT CCCTGGGCCC CAAGGAGGCC AAGAAGGCCT TCCTCGACTT CTGCCATTGC 
TTCCTGGACA AGACGGCGGT TTTGCGGGTG CTAGTCCCTC CCGCCGTCGC CTTTGAACTT 
GACCGCACGC GGCCCGAGGT CATCTCCGAG GACTTGCAGC GGCAGTTTGT ACAGGAGGTA 
GTGCAGAGCC AGCAGGCAGC TGTTAGCCGG CTGCTGGAGG ACTTCCGCTC CAAGCGGCTC 
ATGGGCATGA CACCCTGGGA GCAGGACCTG ACCCAGCTGG AGGCCTGGGT TGGGCGGGAC 
CGTGCCAGCT TTGAGGCCCG GGAGCGGCAC CTGGCTGAAC GGCTGCTGGC CCACCTGGAG 
GAGATGCAAC ACACCATCTC TAACGATGAG GAAAAGAGTG CCGCTGTGGT CAATGCCATC 
AGCCTGTACA TGCGCCACCT TGGGGTGCGG ACCAAGAGCG GAGACAAGAA GTCAGGGATG 
AATTTCTTCC GCAAAAAGGT GATGGGGAAT CGGCGGTCAG ATGAGCCAAC CAAGACCAAA 
AAAGGCCTGA GCATCTTCCT AGACCCTGCT CGCTGGAACC GGGGAGAGCC TCAGGCCTCG 
GATTTCCGAC ACCTCAAAGT CGAGACTGAT GCTGAGAAAC CAGGCCTTAC GGAAAGGAAG 
GGAGGCCTGG GGGTGCCCTC CCGGGACCGG AATGTTGGGG CTCCTGGGCA GGATGTCCCT 
GGAGTTTCTC TGCACCCTCT GCCCGGGGAC AGCCCTGACC GGGAACCAGG TGTTGACACC 
CCGCCAGAGC TGGGGGACCC ACCCCCCCAG GGCCCGGCCA GCCTGGAGCC CCCGGGGCCC 
TCGGAGAGCA CTGAGGAGGG TCCTGACACA GAGAGAAGGT GGAAGAGGCT GTCGGGGCGT 
CTGGGGCGCT CGGAGAGCCT GCGGGTGAGT GACCGCCGCC GGCCTTCCCG GGGCAGCCTC 
GGGGCTAAGG GCCGGGGTGG GGGCCGCTCC CGGAGTGACG TGGACATGGA CCCCAGCTCT 
GCCACGGCCG TGCTTGGCCC TGCCCGACGA GCCACCCCTG AGCCTGGAGA CGAAGGGGAC 
CCTGGGCGGT CAGGACTAGA GCTGGAACCA GAAGAGCCCC CTGGCTGGCG GGAGCTCATC 
CCCCCAGGCA CCCTGCACAG CCTGCCTAAG AGCCAGGTGA AGCGGCAGGA GGTCATCAGC 
GAGCTACTGG TGACAGAGGC TGCCCATGTG CGCATGCTCC GGGTGCTGCA TGACCTCTTC 
TACCAGCCCA TGTTGGAAGG GAACTTCTTC TCCACGGAGG ATCTACAGAA CATCTTCCCC 
AGCCTGGATG AGCTCATCGA GGTGCATTCC CTGTTCCTCG ATCGACTGAT GAAGCGGAGG 
CAGGATAGTG GCTGCCTCAT TGAGGAGATC GGAGATGTAC TGCTGGCCCG GTTTGATGGT 
GCCGAAGGCA AATGGTTCCA GAAAATCTCC TCCCGCTTCT GCAGCCGCCA GTCATTTGCC 
TTAGAGCAGC TCAAAGCCAA GCAGCGCAAG GATCCTCGGT TCTGTGCCTT TGTGCAGGAG 
GCCGAGAGCC GCCCCCGGTG CCGCCGCCTG CAGCTGAAGG ATATGATCCC CACGGAGATG 
CAGCGTCTGA CCAAGTACCC CCTGCTCCTG CAGAGCATTG GGCAGAACAC AGAAGAGTCA 
GCAGAACGGG AGAAAGTAGA ACTGGCAGCT GAGTGCTGCC GGGAAATTCT GCACCATGTT 
AACCAAGCCG TGCGTGACAT GGAGGACCTG CTGCGGCTCA AGGATTATCA GAAGCGCCTG 
GACTTGTCCC TCCTGCGGCA GAGCAGCGAC CCCATGCTAA GCGAGTTCAA GAACCTGGAC 
ATCACCAAAA AGAAGCTGAT CTACGAGGGT CCGCTGACGT GGCGGCTGAC GAAGGACAAG 
GCTGTAGAGG TACACGTGCT GCTGCTGGAT GACCTGCTGC TGCTGCTCCA GCGCCAGGAC 
GATCGGCTGC TGCTCAAGTC CCACAGCCGG ACGCTGACAC CCACACCCGA CGGCAAGACC 
ATGCTGCGGC CGGTGCTGCG GCTCACCTCC GCCATAACCC GCGAGGTGGC TACCGATCAC 
AAAGCCTTCT ATGTCCTTTT TACCTGGGAC CAGGAGGCCC AGATATATGA GCTGGTGGCC 
CAGACGGTGT CGGAACGGAA GAACTGGTGT TCCCTCATCA CTGAAACTGC TGGATCCTTG 
AAGGTCCCTG CCCCCGCCTC TCGCCCCAAA CCCCGGCCCA GCCCAAGCAG CACCCGAGAA 
CCCCTGCTCA GCAGCTCAGA GAATGGCAAC GGTGGTGGCC GAGAGATGCC CCCAGCTGAC 
GGCCAGACGG AGAGAATCCT CAGCACCCTC CTGCCCTACT GCAGGCCCCG CCCTGAGGGC 
CAGCTTGCTG CCAAGGCCCT TGAGAAAGTG CTGTCCCTGA AGCAGCTCCT GTTTCCCTCG 
GAGGAAGACA GTGGGGCAGG GCCTCCCCAG GAAGGGGATG GGGTCCCTGG GGGTGGCCCC 
CTGAACCCGA CACAGACCCA CACCCAGGAA ATTCGGGAAA AGCTGCTCAG CCTGGAGGAG 
ACCATGAAAC AGCTGGAGGA GGTGGAGGAG GAATTTTGTC ACCTGCGACT CCTCCTGTCT 
CAGCTTGGGG AGAACAGCAC CCCCCAGCCT GGGTGTACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100856005 ( XM_014112161.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013967636.1 Canis lupus familiaris rho guanine nucleotide exchange factor 1-like (LOC100856005), transcript variant X2, mRNA.

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CloneID	OCe45889
Clone ID Related Accession (Same CDS sequence)	XM_014112161.1
Accession Version	XM_014112161.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	rho guanine nucleotide exchange factor 1-like isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003727276.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGTT CCCTGGGCCC CAAGGAGGCC AAGAAGGCCT TCCTCGACTT CTGCCATTGC 
TTCCTGGACA AGACGGCGGT TTTGCGGGTG CTAGTCCCTC CCGCCGTCGC CTTTGAACTT 
GACCGCACGC GGCCCGAGGT CATCTCCGAG GACTTGCAGC GGCAGTTTGT ACAGGAGGTA 
GTGCAGAGCC AGCAGGCAGC TGTTAGCCGG CTGCTGGAGG ACTTCCGCTC CAAGCGGCTC 
ATGGGCATGA CACCCTGGGA GCAGGACCTG ACCCAGCTGG AGGCCTGGGT TGGGCGGGAC 
CGTGCCAGCT TTGAGGCCCG GGAGCGGCAC CTGGCTGAAC GGCTGCTGGC CCACCTGGAG 
GAGATGCAAC ACACCATCTC TAACGATGAG GAAAAGAGTG CCGCTGTGGT CAATGCCATC 
AGCCTGTACA TGCGCCACCT TGGGGTGCGG ACCAAGAGCG GAGACAAGAA GTCAGGGATG 
AATTTCTTCC GCAAAAAGGT GATGGGGAAT CGGCGGTCAG ATGAGCCAAC CAAGACCAAA 
AAAGGCCTGA GCATCTTCCT AGACCCTGCT CGCTGGAACC GGGGAGAGCC TCAGGCCTCG 
GATTTCCGAC ACCTCAAAGT CGAGACTGAT GCTGAGAAAC CAGGCCTTAC GGAAAGGAAG 
GGAGGCCTGG GGGTGCCCTC CCGGGACCGG AATGTTGGGG CTCCTGGGCA GGATGTCCCT 
GGAGTTTCTC TGCACCCTCT GCCCGGGGAC AGCCCTGACC GGGAACCAGG TGTTGACACC 
CCGCCAGAGC TGGGGGACCC ACCCCCCCAG GGCCCGGCCA GCCTGGAGCC CCCGGGGCCC 
TCGGAGAGCA CTGAGGAGGG TCCTGACACA GAGAGGCTGT CGGGGCGTCT GGGGCGCTCG 
GAGAGCCTGC GGGTGAGTGA CCGCCGCCGG CCTTCCCGGG GCAGCCTCGG GGCTAAGGGC 
CGGGGTGGGG GCCGCTCCCG GAGTGACGTG GACATGGACC CCAGCTCTGC CACGGCCGTG 
CTTGGCCCTG CCCGACGAGC CACCCCTGAG CCTGGAGACG AAGGGGACCC TGGGCGGTCA 
GGACTAGAGC TGGAACCAGA AGAGCCCCCT GGCTGGCGGG AGCTCATCCC CCCAGGCACC 
CTGCACAGCC TGCCTAAGAG CCAGGTGAAG CGGCAGGAGG TCATCAGCGA GCTACTGGTG 
ACAGAGGCTG CCCATGTGCG CATGCTCCGG GTGCTGCATG ACCTCTTCTA CCAGCCCATG 
TTGGAAGGGA ACTTCTTCTC CACGGAGGAT CTACAGAACA TCTTCCCCAG CCTGGATGAG 
CTCATCGAGG TGCATTCCCT GTTCCTCGAT CGACTGATGA AGCGGAGGCA GGATAGTGGC 
TGCCTCATTG AGGAGATCGG AGATGTACTG CTGGCCCGGT TTGATGGTGC CGAAGGCAAA 
TGGTTCCAGA AAATCTCCTC CCGCTTCTGC AGCCGCCAGT CATTTGCCTT AGAGCAGCTC 
AAAGCCAAGC AGCGCAAGGA TCCTCGGTTC TGTGCCTTTG TGCAGGAGGC CGAGAGCCGC 
CCCCGGTGCC GCCGCCTGCA GCTGAAGGAT ATGATCCCCA CGGAGATGCA GCGTCTGACC 
AAGTACCCCC TGCTCCTGCA GAGCATTGGG CAGAACACAG AAGAGTCAGC AGAACGGGAG 
AAAGTAGAAC TGGCAGCTGA GTGCTGCCGG GAAATTCTGC ACCATGTTAA CCAAGCCGTG 
CGTGACATGG AGGACCTGCT GCGGCTCAAG GATTATCAGA AGCGCCTGGA CTTGTCCCTC 
CTGCGGCAGA GCAGCGACCC CATGCTAAGC GAGTTCAAGA ACCTGGACAT CACCAAAAAG 
AAGCTGATCT ACGAGGGTCC GCTGACGTGG CGGCTGACGA AGGACAAGGC TGTAGAGGTA 
CACGTGCTGC TGCTGGATGA CCTGCTGCTG CTGCTCCAGC GCCAGGACGA TCGGCTGCTG 
CTCAAGTCCC ACAGCCGGAC GCTGACACCC ACACCCGACG GCAAGACCAT GCTGCGGCCG 
GTGCTGCGGC TCACCTCCGC CATAACCCGC GAGGTGGCTA CCGATCACAA AGCCTTCTAT 
GTCCTTTTTA CCTGGGACCA GGAGGCCCAG ATATATGAGC TGGTGGCCCA GACGGTGTCG 
GAACGGAAGA ACTGGTGTTC CCTCATCACT GAAACTGCTG GATCCTTGAA GGTCCCTGCC 
CCCGCCTCTC GCCCCAAACC CCGGCCCAGC CCAAGCAGCA CCCGAGAACC CCTGCTCAGC 
AGCTCAGAGA ATGGCAACGG TGGTGGCCGA GAGATGCCCC CAGCTGACGG CCAGACGGAG 
AGAATCCTCA GCACCCTCCT GCCCTACTGC AGGCCCCGCC CTGAGGGCCA GCTTGCTGCC 
AAGGCCCTTG AGAAAGTGCT GTCCCTGAAG CAGCTCCTGT TTCCCTCGGA GGAAGACAGT 
GGGGCAGGGC CTCCCCAGGA AGGGGATGGG GTCCCTGGGG GTGGCCCCCT GAACCCGACA 
CAGACCCACA CCCAGGAAAT TCGGGAAAAG CTGCTCAGCC TGGAGGAGAC CATGAAACAG 
CTGGAGGAGG TGGAGGAGGA ATTTTGTCAC CTGCGACTCC TCCTGTCTCA GCTTGGGGAG 
AACAGCACCC CCCAGCCTGG GTGTACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967636.1
CDS	457..3126
Translation	MLSSLGPKEAKKAFLDFCHCFLDKTAVLRVLVPPAVAFELDRTRPEVISEDLQRQFVQEVVQSQQAAVSRLLEDFRSKRLMGMTPWEQDLTQLEAWVGRDRASFEARERHLAERLLAHLEEMQHTISNDEEKSAAVVNAISLYMRHLGVRTKSGDKKSGMNFFRKKVMGNRRSDEPTKTKKGLSIFLDPARWNRGEPQASDFRHLKVETDAEKPGLTERKGGLGVPSRDRNVGAPGQDVPGVSLHPLPGDSPDREPGVDTPPELGDPPPQGPASLEPPGPSESTEEGPDTERLSGRLGRSESLRVSDRRRPSRGSLGAKGRGGGRSRSDVDMDPSSATAVLGPARRATPEPGDEGDPGRSGLELEPEEPPGWRELIPPGTLHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDLFYQPMLEGNFFSTEDLQNIFPSLDELIEVHSLFLDRLMKRRQDSGCLIEEIGDVLLARFDGAEGKWFQKISSRFCSRQSFALEQLKAKQRKDPRFCAFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIGQNTEESAEREKVELAAECCREILHHVNQAVRDMEDLLRLKDYQKRLDLSLLRQSSDPMLSEFKNLDITKKKLIYEGPLTWRLTKDKAVEVHVLLLDDLLLLLQRQDDRLLLKSHSRTLTPTPDGKTMLRPVLRLTSAITREVATDHKAFYVLFTWDQEAQIYELVAQTVSERKNWCSLITETAGSLKVPAPASRPKPRPSPSSTREPLLSSSENGNGGGREMPPADGQTERILSTLLPYCRPRPEGQLAAKALEKVLSLKQLLFPSEEDSGAGPPQEGDGVPGGGPLNPTQTHTQEIREKLLSLEETMKQLEEVEEEFCHLRLLLSQLGENSTPQPGCT

Target ORF information:

RefSeq Version	XM_014112161.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris rho guanine nucleotide exchange factor 1-like (LOC100856005), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014112161.1

ORF Insert Sequence:

ATGCTGAGTT CCCTGGGCCC CAAGGAGGCC AAGAAGGCCT TCCTCGACTT CTGCCATTGC 
TTCCTGGACA AGACGGCGGT TTTGCGGGTG CTAGTCCCTC CCGCCGTCGC CTTTGAACTT 
GACCGCACGC GGCCCGAGGT CATCTCCGAG GACTTGCAGC GGCAGTTTGT ACAGGAGGTA 
GTGCAGAGCC AGCAGGCAGC TGTTAGCCGG CTGCTGGAGG ACTTCCGCTC CAAGCGGCTC 
ATGGGCATGA CACCCTGGGA GCAGGACCTG ACCCAGCTGG AGGCCTGGGT TGGGCGGGAC 
CGTGCCAGCT TTGAGGCCCG GGAGCGGCAC CTGGCTGAAC GGCTGCTGGC CCACCTGGAG 
GAGATGCAAC ACACCATCTC TAACGATGAG GAAAAGAGTG CCGCTGTGGT CAATGCCATC 
AGCCTGTACA TGCGCCACCT TGGGGTGCGG ACCAAGAGCG GAGACAAGAA GTCAGGGATG 
AATTTCTTCC GCAAAAAGGT GATGGGGAAT CGGCGGTCAG ATGAGCCAAC CAAGACCAAA 
AAAGGCCTGA GCATCTTCCT AGACCCTGCT CGCTGGAACC GGGGAGAGCC TCAGGCCTCG 
GATTTCCGAC ACCTCAAAGT CGAGACTGAT GCTGAGAAAC CAGGCCTTAC GGAAAGGAAG 
GGAGGCCTGG GGGTGCCCTC CCGGGACCGG AATGTTGGGG CTCCTGGGCA GGATGTCCCT 
GGAGTTTCTC TGCACCCTCT GCCCGGGGAC AGCCCTGACC GGGAACCAGG TGTTGACACC 
CCGCCAGAGC TGGGGGACCC ACCCCCCCAG GGCCCGGCCA GCCTGGAGCC CCCGGGGCCC 
TCGGAGAGCA CTGAGGAGGG TCCTGACACA GAGAGGCTGT CGGGGCGTCT GGGGCGCTCG 
GAGAGCCTGC GGGTGAGTGA CCGCCGCCGG CCTTCCCGGG GCAGCCTCGG GGCTAAGGGC 
CGGGGTGGGG GCCGCTCCCG GAGTGACGTG GACATGGACC CCAGCTCTGC CACGGCCGTG 
CTTGGCCCTG CCCGACGAGC CACCCCTGAG CCTGGAGACG AAGGGGACCC TGGGCGGTCA 
GGACTAGAGC TGGAACCAGA AGAGCCCCCT GGCTGGCGGG AGCTCATCCC CCCAGGCACC 
CTGCACAGCC TGCCTAAGAG CCAGGTGAAG CGGCAGGAGG TCATCAGCGA GCTACTGGTG 
ACAGAGGCTG CCCATGTGCG CATGCTCCGG GTGCTGCATG ACCTCTTCTA CCAGCCCATG 
TTGGAAGGGA ACTTCTTCTC CACGGAGGAT CTACAGAACA TCTTCCCCAG CCTGGATGAG 
CTCATCGAGG TGCATTCCCT GTTCCTCGAT CGACTGATGA AGCGGAGGCA GGATAGTGGC 
TGCCTCATTG AGGAGATCGG AGATGTACTG CTGGCCCGGT TTGATGGTGC CGAAGGCAAA 
TGGTTCCAGA AAATCTCCTC CCGCTTCTGC AGCCGCCAGT CATTTGCCTT AGAGCAGCTC 
AAAGCCAAGC AGCGCAAGGA TCCTCGGTTC TGTGCCTTTG TGCAGGAGGC CGAGAGCCGC 
CCCCGGTGCC GCCGCCTGCA GCTGAAGGAT ATGATCCCCA CGGAGATGCA GCGTCTGACC 
AAGTACCCCC TGCTCCTGCA GAGCATTGGG CAGAACACAG AAGAGTCAGC AGAACGGGAG 
AAAGTAGAAC TGGCAGCTGA GTGCTGCCGG GAAATTCTGC ACCATGTTAA CCAAGCCGTG 
CGTGACATGG AGGACCTGCT GCGGCTCAAG GATTATCAGA AGCGCCTGGA CTTGTCCCTC 
CTGCGGCAGA GCAGCGACCC CATGCTAAGC GAGTTCAAGA ACCTGGACAT CACCAAAAAG 
AAGCTGATCT ACGAGGGTCC GCTGACGTGG CGGCTGACGA AGGACAAGGC TGTAGAGGTA 
CACGTGCTGC TGCTGGATGA CCTGCTGCTG CTGCTCCAGC GCCAGGACGA TCGGCTGCTG 
CTCAAGTCCC ACAGCCGGAC GCTGACACCC ACACCCGACG GCAAGACCAT GCTGCGGCCG 
GTGCTGCGGC TCACCTCCGC CATAACCCGC GAGGTGGCTA CCGATCACAA AGCCTTCTAT 
GTCCTTTTTA CCTGGGACCA GGAGGCCCAG ATATATGAGC TGGTGGCCCA GACGGTGTCG 
GAACGGAAGA ACTGGTGTTC CCTCATCACT GAAACTGCTG GATCCTTGAA GGTCCCTGCC 
CCCGCCTCTC GCCCCAAACC CCGGCCCAGC CCAAGCAGCA CCCGAGAACC CCTGCTCAGC 
AGCTCAGAGA ATGGCAACGG TGGTGGCCGA GAGATGCCCC CAGCTGACGG CCAGACGGAG 
AGAATCCTCA GCACCCTCCT GCCCTACTGC AGGCCCCGCC CTGAGGGCCA GCTTGCTGCC 
AAGGCCCTTG AGAAAGTGCT GTCCCTGAAG CAGCTCCTGT TTCCCTCGGA GGAAGACAGT 
GGGGCAGGGC CTCCCCAGGA AGGGGATGGG GTCCCTGGGG GTGGCCCCCT GAACCCGACA 
CAGACCCACA CCCAGGAAAT TCGGGAAAAG CTGCTCAGCC TGGAGGAGAC CATGAAACAG 
CTGGAGGAGG TGGAGGAGGA ATTTTGTCAC CTGCGACTCC TCCTGTCTCA GCTTGGGGAG 
AACAGCACCC CCCAGCCTGG GTGTACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100856005 ( XM_014112162.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013967637.1 Canis lupus familiaris rho guanine nucleotide exchange factor 1-like (LOC100856005), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe45890
Clone ID Related Accession (Same CDS sequence)	XM_014112162.2 , XM_014112162.1
Accession Version	XM_014112162.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	rho guanine nucleotide exchange factor 1-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003727276.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014112162.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGTT CCCTGGGCCC CAAGGAGGCC AAGAAGGCCT TCCTCGACTT CTGCCATTGC 
TTCCTGGACA AGACGGCGGT TTTGCGGGTG CTAGTCCCTC CCGCCGTCGC CTTTGAACTT 
GACCGCACGC GGCCCGAGGT CATCTCCGAG GACTTGCAGC GGCAGTTTGT ACAGGAGGTA 
GTGCAGAGCC AGCAGGCAGC TGTTAGCCGG CTGCTGGAGG ACTTCCGCTC CAAGCGGCTC 
ATGGGCATGA CACCCTGGGA GCAGGACCTG ACCCAGCTGG AGGCCTGGGT TGGGCGGGAC 
CGTGCCAGCT TTGAGGCCCG GGAGCGGCAC CTGGCTGAAC GGCTGCTGGC CCACCTGGAG 
GAGATGCAAC ACACCATCTC TAACGATGAG GAAAAGAGTG CCGCTGTGGT CAATGCCATC 
AGCCTGTACA TGCGCCACCT TGGGGTGCGG ACCAAGAGCG GAGACAAGAA GTCAGGGATG 
AATTTCTTCC GCAAAAAGGT GATGGGGAAT CGGCGGTCAG ATGAGCCAAC CAAGACCAAA 
AAAGGCCTGA GCATCTTCCT AGACCCTGCT CGCTGGAACC GGGGAGAGCC TCAGGCCTCG 
GATTTCCGAC ACCTCAAAGT CGAGACTGAT GCTGAGAAAC CAGGCCTTAC GGAAAGGAAG 
GGAGGCCTGG GGGTGCCCTC CCGGGACCGG AATGTTGGGG CTCCTGGGCA GGATGTCCCT 
GGAGTTTCTC TGCACCCTCT GCCCGGGGAC AGCCCTGACC GGGAACCAGG TGTTGACACC 
CCGCCAGAGC TGGGGGACCC ACCCCCCCAG GGCCCGGCCA GCCTGGAGCC CCCGGGGCCC 
TCGGAGAGCA CTGAGGAGGG TCCTGACACA GAGAGAAGGT GGAAGAGGCT GTCGGGGCGT 
CTGGGGCGCT CGGAGAGCCT GCGGGTGAGT GACCGCCGCC GGCCTTCCCG GGGCAGCCTC 
GGGGCTAAGG GCCGGGGTGG GGGCCGCTCC CGGAGTGACG TGGACATGGA CCCCAGCTCT 
GCCACGGCCG TGCTTGGCCC TGCCCGACGA GCCACCCCTG AGCCTGGAGA CGAAGGGGAC 
CCTGGGCGGT CAGGACTAGA GCTGGAACCA GAAGAGCCCC CTGGCTGGCG GGAGCTCATC 
CCCCCAGGCA CCCTGCACAG CCTGCCTAAG AGCCAGGTGA AGCGGCAGGA GGTCATCAGC 
GAGCTACTGG TGACAGAGGC TGCCCATGTG CGCATGCTCC GGGTGCTGCA TGACCTCTTC 
TACCAGCCCA TGTTGGAAGG GAACTTCTTC TCCACGGAGG ATCTACAGAA CATCTTCCCC 
AGCCTGGATG AGCTCATCGA GGTGCATTCC CTGTTCCTCG ATCGACTGAT GAAGCGGAGG 
CAGGATAGTG GCTGCCTCAT TGAGGAGATC GGAGATGTAC TGCTGGCCCG GTTTGATGGT 
GCCGAAGGCA AATGGTTCCA GAAAATCTCC TCCCGCTTCT GCAGCCGCCA GTCATTTGCC 
TTAGAGCAGC TCAAAGCCAA GCAGCGCAAG GATCCTCGGT TCTGTGCCTT TGTGCAGGAG 
GCCGAGAGCC GCCCCCGGTG CCGCCGCCTG CAGCTGAAGG ATATGATCCC CACGGAGATG 
CAGCGTCTGA CCAAGTACCC CCTGCTCCTG CAGAGCATTG GGCAGAACAC AGAAGAGTCA 
GCAGAACGGG AGAAAGTAGA ACTGGCAGCT GAGTGCTGCC GGGAAATTCT GCACCATGTT 
AACCAAGCCG TGCGTGACAT GGAGGACCTG CTGCGGCTCA AGGATTATCA GAAGCGCCTG 
GACTTGTCCC TCCTGCGGCA GAGCAGCGAC CCCATGCTAA GCGAGTTCAA GAACCTGGAC 
ATCACCAAAA AGAAGCTGAT CTACGAGGGT CCGCTGACGT GGCGGCTGAC GAAGGACAAG 
GCTGTAGAGG TACACGTGCT GCTGCTGGAT GACCTGCTGC TGCTGCTCCA GCGCCAGGAC 
GATCGGCTGC TGCTCAAGTC CCACAGCCGG ACGCTGACAC CCACACCCGA CGGCAAGACC 
ATGCTGCGGC CGGTGCTGCG GCTCACCTCC GCCATAACCC GCGAGGTGGC TACCGATCAC 
AAAGCCTTCT ATGTCCTTTT TACCTGGGAC CAGGAGGCCC AGATATATGA GCTGGTGGCC 
CAGACGGTGT CGGAACGGAA GAACTGGTGT TCCCTCATCA CTGAAACTGC TGGATCCTTG 
AAGGTCCCTG CCCCCGCCTC TCGCCCCAAA CCCCGGCCCA GCCCAAGCAG CACCCGAGAA 
CCCCTGCTCA GCAGCTCAGA GAATGGCAAC GGTGGTGGCC GAGAGATGCC CCCAGCTGAC 
GGCCAGACGG AGAGAATCCT CAGCACCCTC CTGCCCTACT GCAGGCCCCG CCCTGAGGGC 
CAGCTTGCTG CCAAGGCCCT TGAGAAAGGA GGTGGAGGAG GAATTTTGTC ACCTGCGACT 
CCTCCTGTCT CAGCTTGGGG AGAACAGCAC CCCCCAGCCT GGGTGTACCT GAGGCTCCTT 
GGGCCAGGCA GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967637.1
CDS	489..3023
Translation	MLSSLGPKEAKKAFLDFCHCFLDKTAVLRVLVPPAVAFELDRTRPEVISEDLQRQFVQEVVQSQQAAVSRLLEDFRSKRLMGMTPWEQDLTQLEAWVGRDRASFEARERHLAERLLAHLEEMQHTISNDEEKSAAVVNAISLYMRHLGVRTKSGDKKSGMNFFRKKVMGNRRSDEPTKTKKGLSIFLDPARWNRGEPQASDFRHLKVETDAEKPGLTERKGGLGVPSRDRNVGAPGQDVPGVSLHPLPGDSPDREPGVDTPPELGDPPPQGPASLEPPGPSESTEEGPDTERRWKRLSGRLGRSESLRVSDRRRPSRGSLGAKGRGGGRSRSDVDMDPSSATAVLGPARRATPEPGDEGDPGRSGLELEPEEPPGWRELIPPGTLHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDLFYQPMLEGNFFSTEDLQNIFPSLDELIEVHSLFLDRLMKRRQDSGCLIEEIGDVLLARFDGAEGKWFQKISSRFCSRQSFALEQLKAKQRKDPRFCAFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIGQNTEESAEREKVELAAECCREILHHVNQAVRDMEDLLRLKDYQKRLDLSLLRQSSDPMLSEFKNLDITKKKLIYEGPLTWRLTKDKAVEVHVLLLDDLLLLLQRQDDRLLLKSHSRTLTPTPDGKTMLRPVLRLTSAITREVATDHKAFYVLFTWDQEAQIYELVAQTVSERKNWCSLITETAGSLKVPAPASRPKPRPSPSSTREPLLSSSENGNGGGREMPPADGQTERILSTLLPYCRPRPEGQLAAKALEKGGGGGILSPATPPVSAWGEQHPPAWVYLRLLGPGR

Target ORF information:

RefSeq Version	XM_014112162.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris rho guanine nucleotide exchange factor 1-like (LOC100856005), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014112162.2

ORF Insert Sequence:

ATGCTGAGTT CCCTGGGCCC CAAGGAGGCC AAGAAGGCCT TCCTCGACTT CTGCCATTGC 
TTCCTGGACA AGACGGCGGT TTTGCGGGTG CTAGTCCCTC CCGCCGTCGC CTTTGAACTT 
GACCGCACGC GGCCCGAGGT CATCTCCGAG GACTTGCAGC GGCAGTTTGT ACAGGAGGTA 
GTGCAGAGCC AGCAGGCAGC TGTTAGCCGG CTGCTGGAGG ACTTCCGCTC CAAGCGGCTC 
ATGGGCATGA CACCCTGGGA GCAGGACCTG ACCCAGCTGG AGGCCTGGGT TGGGCGGGAC 
CGTGCCAGCT TTGAGGCCCG GGAGCGGCAC CTGGCTGAAC GGCTGCTGGC CCACCTGGAG 
GAGATGCAAC ACACCATCTC TAACGATGAG GAAAAGAGTG CCGCTGTGGT CAATGCCATC 
AGCCTGTACA TGCGCCACCT TGGGGTGCGG ACCAAGAGCG GAGACAAGAA GTCAGGGATG 
AATTTCTTCC GCAAAAAGGT GATGGGGAAT CGGCGGTCAG ATGAGCCAAC CAAGACCAAA 
AAAGGCCTGA GCATCTTCCT AGACCCTGCT CGCTGGAACC GGGGAGAGCC TCAGGCCTCG 
GATTTCCGAC ACCTCAAAGT CGAGACTGAT GCTGAGAAAC CAGGCCTTAC GGAAAGGAAG 
GGAGGCCTGG GGGTGCCCTC CCGGGACCGG AATGTTGGGG CTCCTGGGCA GGATGTCCCT 
GGAGTTTCTC TGCACCCTCT GCCCGGGGAC AGCCCTGACC GGGAACCAGG TGTTGACACC 
CCGCCAGAGC TGGGGGACCC ACCCCCCCAG GGCCCGGCCA GCCTGGAGCC CCCGGGGCCC 
TCGGAGAGCA CTGAGGAGGG TCCTGACACA GAGAGAAGGT GGAAGAGGCT GTCGGGGCGT 
CTGGGGCGCT CGGAGAGCCT GCGGGTGAGT GACCGCCGCC GGCCTTCCCG GGGCAGCCTC 
GGGGCTAAGG GCCGGGGTGG GGGCCGCTCC CGGAGTGACG TGGACATGGA CCCCAGCTCT 
GCCACGGCCG TGCTTGGCCC TGCCCGACGA GCCACCCCTG AGCCTGGAGA CGAAGGGGAC 
CCTGGGCGGT CAGGACTAGA GCTGGAACCA GAAGAGCCCC CTGGCTGGCG GGAGCTCATC 
CCCCCAGGCA CCCTGCACAG CCTGCCTAAG AGCCAGGTGA AGCGGCAGGA GGTCATCAGC 
GAGCTACTGG TGACAGAGGC TGCCCATGTG CGCATGCTCC GGGTGCTGCA TGACCTCTTC 
TACCAGCCCA TGTTGGAAGG GAACTTCTTC TCCACGGAGG ATCTACAGAA CATCTTCCCC 
AGCCTGGATG AGCTCATCGA GGTGCATTCC CTGTTCCTCG ATCGACTGAT GAAGCGGAGG 
CAGGATAGTG GCTGCCTCAT TGAGGAGATC GGAGATGTAC TGCTGGCCCG GTTTGATGGT 
GCCGAAGGCA AATGGTTCCA GAAAATCTCC TCCCGCTTCT GCAGCCGCCA GTCATTTGCC 
TTAGAGCAGC TCAAAGCCAA GCAGCGCAAG GATCCTCGGT TCTGTGCCTT TGTGCAGGAG 
GCCGAGAGCC GCCCCCGGTG CCGCCGCCTG CAGCTGAAGG ATATGATCCC CACGGAGATG 
CAGCGTCTGA CCAAGTACCC CCTGCTCCTG CAGAGCATTG GGCAGAACAC AGAAGAGTCA 
GCAGAACGGG AGAAAGTAGA ACTGGCAGCT GAGTGCTGCC GGGAAATTCT GCACCATGTT 
AACCAAGCCG TGCGTGACAT GGAGGACCTG CTGCGGCTCA AGGATTATCA GAAGCGCCTG 
GACTTGTCCC TCCTGCGGCA GAGCAGCGAC CCCATGCTAA GCGAGTTCAA GAACCTGGAC 
ATCACCAAAA AGAAGCTGAT CTACGAGGGT CCGCTGACGT GGCGGCTGAC GAAGGACAAG 
GCTGTAGAGG TACACGTGCT GCTGCTGGAT GACCTGCTGC TGCTGCTCCA GCGCCAGGAC 
GATCGGCTGC TGCTCAAGTC CCACAGCCGG ACGCTGACAC CCACACCCGA CGGCAAGACC 
ATGCTGCGGC CGGTGCTGCG GCTCACCTCC GCCATAACCC GCGAGGTGGC TACCGATCAC 
AAAGCCTTCT ATGTCCTTTT TACCTGGGAC CAGGAGGCCC AGATATATGA GCTGGTGGCC 
CAGACGGTGT CGGAACGGAA GAACTGGTGT TCCCTCATCA CTGAAACTGC TGGATCCTTG 
AAGGTCCCTG CCCCCGCCTC TCGCCCCAAA CCCCGGCCCA GCCCAAGCAG CACCCGAGAA 
CCCCTGCTCA GCAGCTCAGA GAATGGCAAC GGTGGTGGCC GAGAGATGCC CCCAGCTGAC 
GGCCAGACGG AGAGAATCCT CAGCACCCTC CTGCCCTACT GCAGGCCCCG CCCTGAGGGC 
CAGCTTGCTG CCAAGGCCCT TGAGAAAGGA GGTGGAGGAG GAATTTTGTC ACCTGCGACT 
CCTCCTGTCT CAGCTTGGGG AGAACAGCAC CCCCCAGCCT GGGTGTACCT GAGGCTCCTT 
GGGCCAGGCA GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100856005 ( XM_014112162.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013967637.1 Canis lupus familiaris rho guanine nucleotide exchange factor 1-like (LOC100856005), transcript variant X3, mRNA.

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CloneID	OCe45890
Clone ID Related Accession (Same CDS sequence)	XM_014112162.2 , XM_014112162.1
Accession Version	XM_014112162.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	rho guanine nucleotide exchange factor 1-like isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003727276.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGTT CCCTGGGCCC CAAGGAGGCC AAGAAGGCCT TCCTCGACTT CTGCCATTGC 
TTCCTGGACA AGACGGCGGT TTTGCGGGTG CTAGTCCCTC CCGCCGTCGC CTTTGAACTT 
GACCGCACGC GGCCCGAGGT CATCTCCGAG GACTTGCAGC GGCAGTTTGT ACAGGAGGTA 
GTGCAGAGCC AGCAGGCAGC TGTTAGCCGG CTGCTGGAGG ACTTCCGCTC CAAGCGGCTC 
ATGGGCATGA CACCCTGGGA GCAGGACCTG ACCCAGCTGG AGGCCTGGGT TGGGCGGGAC 
CGTGCCAGCT TTGAGGCCCG GGAGCGGCAC CTGGCTGAAC GGCTGCTGGC CCACCTGGAG 
GAGATGCAAC ACACCATCTC TAACGATGAG GAAAAGAGTG CCGCTGTGGT CAATGCCATC 
AGCCTGTACA TGCGCCACCT TGGGGTGCGG ACCAAGAGCG GAGACAAGAA GTCAGGGATG 
AATTTCTTCC GCAAAAAGGT GATGGGGAAT CGGCGGTCAG ATGAGCCAAC CAAGACCAAA 
AAAGGCCTGA GCATCTTCCT AGACCCTGCT CGCTGGAACC GGGGAGAGCC TCAGGCCTCG 
GATTTCCGAC ACCTCAAAGT CGAGACTGAT GCTGAGAAAC CAGGCCTTAC GGAAAGGAAG 
GGAGGCCTGG GGGTGCCCTC CCGGGACCGG AATGTTGGGG CTCCTGGGCA GGATGTCCCT 
GGAGTTTCTC TGCACCCTCT GCCCGGGGAC AGCCCTGACC GGGAACCAGG TGTTGACACC 
CCGCCAGAGC TGGGGGACCC ACCCCCCCAG GGCCCGGCCA GCCTGGAGCC CCCGGGGCCC 
TCGGAGAGCA CTGAGGAGGG TCCTGACACA GAGAGAAGGT GGAAGAGGCT GTCGGGGCGT 
CTGGGGCGCT CGGAGAGCCT GCGGGTGAGT GACCGCCGCC GGCCTTCCCG GGGCAGCCTC 
GGGGCTAAGG GCCGGGGTGG GGGCCGCTCC CGGAGTGACG TGGACATGGA CCCCAGCTCT 
GCCACGGCCG TGCTTGGCCC TGCCCGACGA GCCACCCCTG AGCCTGGAGA CGAAGGGGAC 
CCTGGGCGGT CAGGACTAGA GCTGGAACCA GAAGAGCCCC CTGGCTGGCG GGAGCTCATC 
CCCCCAGGCA CCCTGCACAG CCTGCCTAAG AGCCAGGTGA AGCGGCAGGA GGTCATCAGC 
GAGCTACTGG TGACAGAGGC TGCCCATGTG CGCATGCTCC GGGTGCTGCA TGACCTCTTC 
TACCAGCCCA TGTTGGAAGG GAACTTCTTC TCCACGGAGG ATCTACAGAA CATCTTCCCC 
AGCCTGGATG AGCTCATCGA GGTGCATTCC CTGTTCCTCG ATCGACTGAT GAAGCGGAGG 
CAGGATAGTG GCTGCCTCAT TGAGGAGATC GGAGATGTAC TGCTGGCCCG GTTTGATGGT 
GCCGAAGGCA AATGGTTCCA GAAAATCTCC TCCCGCTTCT GCAGCCGCCA GTCATTTGCC 
TTAGAGCAGC TCAAAGCCAA GCAGCGCAAG GATCCTCGGT TCTGTGCCTT TGTGCAGGAG 
GCCGAGAGCC GCCCCCGGTG CCGCCGCCTG CAGCTGAAGG ATATGATCCC CACGGAGATG 
CAGCGTCTGA CCAAGTACCC CCTGCTCCTG CAGAGCATTG GGCAGAACAC AGAAGAGTCA 
GCAGAACGGG AGAAAGTAGA ACTGGCAGCT GAGTGCTGCC GGGAAATTCT GCACCATGTT 
AACCAAGCCG TGCGTGACAT GGAGGACCTG CTGCGGCTCA AGGATTATCA GAAGCGCCTG 
GACTTGTCCC TCCTGCGGCA GAGCAGCGAC CCCATGCTAA GCGAGTTCAA GAACCTGGAC 
ATCACCAAAA AGAAGCTGAT CTACGAGGGT CCGCTGACGT GGCGGCTGAC GAAGGACAAG 
GCTGTAGAGG TACACGTGCT GCTGCTGGAT GACCTGCTGC TGCTGCTCCA GCGCCAGGAC 
GATCGGCTGC TGCTCAAGTC CCACAGCCGG ACGCTGACAC CCACACCCGA CGGCAAGACC 
ATGCTGCGGC CGGTGCTGCG GCTCACCTCC GCCATAACCC GCGAGGTGGC TACCGATCAC 
AAAGCCTTCT ATGTCCTTTT TACCTGGGAC CAGGAGGCCC AGATATATGA GCTGGTGGCC 
CAGACGGTGT CGGAACGGAA GAACTGGTGT TCCCTCATCA CTGAAACTGC TGGATCCTTG 
AAGGTCCCTG CCCCCGCCTC TCGCCCCAAA CCCCGGCCCA GCCCAAGCAG CACCCGAGAA 
CCCCTGCTCA GCAGCTCAGA GAATGGCAAC GGTGGTGGCC GAGAGATGCC CCCAGCTGAC 
GGCCAGACGG AGAGAATCCT CAGCACCCTC CTGCCCTACT GCAGGCCCCG CCCTGAGGGC 
CAGCTTGCTG CCAAGGCCCT TGAGAAAGGA GGTGGAGGAG GAATTTTGTC ACCTGCGACT 
CCTCCTGTCT CAGCTTGGGG AGAACAGCAC CCCCCAGCCT GGGTGTACCT GAGGCTCCTT 
GGGCCAGGCA GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967637.1
CDS	460..2994
Translation	MLSSLGPKEAKKAFLDFCHCFLDKTAVLRVLVPPAVAFELDRTRPEVISEDLQRQFVQEVVQSQQAAVSRLLEDFRSKRLMGMTPWEQDLTQLEAWVGRDRASFEARERHLAERLLAHLEEMQHTISNDEEKSAAVVNAISLYMRHLGVRTKSGDKKSGMNFFRKKVMGNRRSDEPTKTKKGLSIFLDPARWNRGEPQASDFRHLKVETDAEKPGLTERKGGLGVPSRDRNVGAPGQDVPGVSLHPLPGDSPDREPGVDTPPELGDPPPQGPASLEPPGPSESTEEGPDTERRWKRLSGRLGRSESLRVSDRRRPSRGSLGAKGRGGGRSRSDVDMDPSSATAVLGPARRATPEPGDEGDPGRSGLELEPEEPPGWRELIPPGTLHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDLFYQPMLEGNFFSTEDLQNIFPSLDELIEVHSLFLDRLMKRRQDSGCLIEEIGDVLLARFDGAEGKWFQKISSRFCSRQSFALEQLKAKQRKDPRFCAFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIGQNTEESAEREKVELAAECCREILHHVNQAVRDMEDLLRLKDYQKRLDLSLLRQSSDPMLSEFKNLDITKKKLIYEGPLTWRLTKDKAVEVHVLLLDDLLLLLQRQDDRLLLKSHSRTLTPTPDGKTMLRPVLRLTSAITREVATDHKAFYVLFTWDQEAQIYELVAQTVSERKNWCSLITETAGSLKVPAPASRPKPRPSPSSTREPLLSSSENGNGGGREMPPADGQTERILSTLLPYCRPRPEGQLAAKALEKGGGGGILSPATPPVSAWGEQHPPAWVYLRLLGPGR

Target ORF information:

RefSeq Version	XM_014112162.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris rho guanine nucleotide exchange factor 1-like (LOC100856005), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014112162.1

ORF Insert Sequence:

ATGCTGAGTT CCCTGGGCCC CAAGGAGGCC AAGAAGGCCT TCCTCGACTT CTGCCATTGC 
TTCCTGGACA AGACGGCGGT TTTGCGGGTG CTAGTCCCTC CCGCCGTCGC CTTTGAACTT 
GACCGCACGC GGCCCGAGGT CATCTCCGAG GACTTGCAGC GGCAGTTTGT ACAGGAGGTA 
GTGCAGAGCC AGCAGGCAGC TGTTAGCCGG CTGCTGGAGG ACTTCCGCTC CAAGCGGCTC 
ATGGGCATGA CACCCTGGGA GCAGGACCTG ACCCAGCTGG AGGCCTGGGT TGGGCGGGAC 
CGTGCCAGCT TTGAGGCCCG GGAGCGGCAC CTGGCTGAAC GGCTGCTGGC CCACCTGGAG 
GAGATGCAAC ACACCATCTC TAACGATGAG GAAAAGAGTG CCGCTGTGGT CAATGCCATC 
AGCCTGTACA TGCGCCACCT TGGGGTGCGG ACCAAGAGCG GAGACAAGAA GTCAGGGATG 
AATTTCTTCC GCAAAAAGGT GATGGGGAAT CGGCGGTCAG ATGAGCCAAC CAAGACCAAA 
AAAGGCCTGA GCATCTTCCT AGACCCTGCT CGCTGGAACC GGGGAGAGCC TCAGGCCTCG 
GATTTCCGAC ACCTCAAAGT CGAGACTGAT GCTGAGAAAC CAGGCCTTAC GGAAAGGAAG 
GGAGGCCTGG GGGTGCCCTC CCGGGACCGG AATGTTGGGG CTCCTGGGCA GGATGTCCCT 
GGAGTTTCTC TGCACCCTCT GCCCGGGGAC AGCCCTGACC GGGAACCAGG TGTTGACACC 
CCGCCAGAGC TGGGGGACCC ACCCCCCCAG GGCCCGGCCA GCCTGGAGCC CCCGGGGCCC 
TCGGAGAGCA CTGAGGAGGG TCCTGACACA GAGAGAAGGT GGAAGAGGCT GTCGGGGCGT 
CTGGGGCGCT CGGAGAGCCT GCGGGTGAGT GACCGCCGCC GGCCTTCCCG GGGCAGCCTC 
GGGGCTAAGG GCCGGGGTGG GGGCCGCTCC CGGAGTGACG TGGACATGGA CCCCAGCTCT 
GCCACGGCCG TGCTTGGCCC TGCCCGACGA GCCACCCCTG AGCCTGGAGA CGAAGGGGAC 
CCTGGGCGGT CAGGACTAGA GCTGGAACCA GAAGAGCCCC CTGGCTGGCG GGAGCTCATC 
CCCCCAGGCA CCCTGCACAG CCTGCCTAAG AGCCAGGTGA AGCGGCAGGA GGTCATCAGC 
GAGCTACTGG TGACAGAGGC TGCCCATGTG CGCATGCTCC GGGTGCTGCA TGACCTCTTC 
TACCAGCCCA TGTTGGAAGG GAACTTCTTC TCCACGGAGG ATCTACAGAA CATCTTCCCC 
AGCCTGGATG AGCTCATCGA GGTGCATTCC CTGTTCCTCG ATCGACTGAT GAAGCGGAGG 
CAGGATAGTG GCTGCCTCAT TGAGGAGATC GGAGATGTAC TGCTGGCCCG GTTTGATGGT 
GCCGAAGGCA AATGGTTCCA GAAAATCTCC TCCCGCTTCT GCAGCCGCCA GTCATTTGCC 
TTAGAGCAGC TCAAAGCCAA GCAGCGCAAG GATCCTCGGT TCTGTGCCTT TGTGCAGGAG 
GCCGAGAGCC GCCCCCGGTG CCGCCGCCTG CAGCTGAAGG ATATGATCCC CACGGAGATG 
CAGCGTCTGA CCAAGTACCC CCTGCTCCTG CAGAGCATTG GGCAGAACAC AGAAGAGTCA 
GCAGAACGGG AGAAAGTAGA ACTGGCAGCT GAGTGCTGCC GGGAAATTCT GCACCATGTT 
AACCAAGCCG TGCGTGACAT GGAGGACCTG CTGCGGCTCA AGGATTATCA GAAGCGCCTG 
GACTTGTCCC TCCTGCGGCA GAGCAGCGAC CCCATGCTAA GCGAGTTCAA GAACCTGGAC 
ATCACCAAAA AGAAGCTGAT CTACGAGGGT CCGCTGACGT GGCGGCTGAC GAAGGACAAG 
GCTGTAGAGG TACACGTGCT GCTGCTGGAT GACCTGCTGC TGCTGCTCCA GCGCCAGGAC 
GATCGGCTGC TGCTCAAGTC CCACAGCCGG ACGCTGACAC CCACACCCGA CGGCAAGACC 
ATGCTGCGGC CGGTGCTGCG GCTCACCTCC GCCATAACCC GCGAGGTGGC TACCGATCAC 
AAAGCCTTCT ATGTCCTTTT TACCTGGGAC CAGGAGGCCC AGATATATGA GCTGGTGGCC 
CAGACGGTGT CGGAACGGAA GAACTGGTGT TCCCTCATCA CTGAAACTGC TGGATCCTTG 
AAGGTCCCTG CCCCCGCCTC TCGCCCCAAA CCCCGGCCCA GCCCAAGCAG CACCCGAGAA 
CCCCTGCTCA GCAGCTCAGA GAATGGCAAC GGTGGTGGCC GAGAGATGCC CCCAGCTGAC 
GGCCAGACGG AGAGAATCCT CAGCACCCTC CTGCCCTACT GCAGGCCCCG CCCTGAGGGC 
CAGCTTGCTG CCAAGGCCCT TGAGAAAGGA GGTGGAGGAG GAATTTTGTC ACCTGCGACT 
CCTCCTGTCT CAGCTTGGGG AGAACAGCAC CCCCCAGCCT GGGTGTACCT GAGGCTCCTT 
GGGCCAGGCA GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100856005 ( XM_014112163.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013967638.1 Canis lupus familiaris rho guanine nucleotide exchange factor 1-like (LOC100856005), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe45891
Clone ID Related Accession (Same CDS sequence)	XM_014112163.2 , XM_014112163.1
Accession Version	XM_014112163.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2502bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	rho guanine nucleotide exchange factor 1-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003727276.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014112163.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGTT CCCTGGGCCC CAAGGAGGCC AAGAAGGCCT TCCTCGACTT CTGCCATTGC 
TTCCTGGACA AGACGGCGGT TTTGCGGGTG CTAGTCCCTC CCGCCGTCGC CTTTGAACTT 
GACCGCACGC GGCCCGAGGT CATCTCCGAG GACTTGCAGC GGCAGTTTGT ACAGGAGGTA 
GTGCAGAGCC AGCAGGCAGC TGTTAGCCGG CTGCTGGAGG ACTTCCGCTC CAAGCGGCTC 
ATGGGCATGA CACCCTGGGA GCAGGACCTG ACCCAGCTGG AGGCCTGGGT TGGGCGGGAC 
CGTGCCAGCT TTGAGGCCCG GGAGCGGCAC CTGGCTGAAC GGCTGCTGGC CCACCTGGAG 
GAGATGCAAC ACACCATCTC TAACGATGAG GAAAAGAGTG CCGCTGTGGT CAATGCCATC 
AGCCTGTACA TGCGCCACCT TGGGGTGCGG ACCAAGAGCG GAGACAAGAA GTCAGGGATG 
AATTTCTTCC GCAAAAAGGT GATGGGGAAT CGGCGGTCAG ATGAGCCAAC CAAGACCAAA 
AAAGGCCTGA GCATCTTCCT AGACCCTGCT CGCTGGAACC GGGGAGAGCC TCAGGCCTCG 
GATTTCCGAC ACCTCAAAGT CGAGACTGAT GCTGAGAAAC CAGGCCTTAC GGAAAGGAAG 
GGAGGCCTGG GGGTGCCCTC CCGGGACCGG AATGTTGGGG CTCCTGGGCA GGATGTCCCT 
GGAGTTTCTC TGCACCCTCT GCCCGGGGAC AGCCCTGACC GGGAACCAGG TGTTGACACC 
CCGCCAGAGC TGGGGGACCC ACCCCCCCAG GGCCCGGCCA GCCTGGAGCC CCCGGGGCCC 
TCGGAGAGCA CTGAGGAGGG TCCTGACACA GAGAGCCCTG AGCCTGGAGA CGAAGGGGAC 
CCTGGGCGGT CAGGACTAGA GCTGGAACCA GAAGAGCCCC CTGGCTGGCG GGAGCTCATC 
CCCCCAGGCA CCCTGCACAG CCTGCCTAAG AGCCAGGTGA AGCGGCAGGA GGTCATCAGC 
GAGCTACTGG TGACAGAGGC TGCCCATGTG CGCATGCTCC GGGTGCTGCA TGACCTCTTC 
TACCAGCCCA TGTTGGAAGG GAACTTCTTC TCCACGGAGG ATCTACAGAA CATCTTCCCC 
AGCCTGGATG AGCTCATCGA GGTGCATTCC CTGTTCCTCG ATCGACTGAT GAAGCGGAGG 
CAGGATAGTG GCTGCCTCAT TGAGGAGATC GGAGATGTAC TGCTGGCCCG GTTTGATGGT 
GCCGAAGGCA AATGGTTCCA GAAAATCTCC TCCCGCTTCT GCAGCCGCCA GTCATTTGCC 
TTAGAGCAGC TCAAAGCCAA GCAGCGCAAG GATCCTCGGT TCTGTGCCTT TGTGCAGGAG 
GCCGAGAGCC GCCCCCGGTG CCGCCGCCTG CAGCTGAAGG ATATGATCCC CACGGAGATG 
CAGCGTCTGA CCAAGTACCC CCTGCTCCTG CAGAGCATTG GGCAGAACAC AGAAGAGTCA 
GCAGAACGGG AGAAAGTAGA ACTGGCAGCT GAGTGCTGCC GGGAAATTCT GCACCATGTT 
AACCAAGCCG TGCGTGACAT GGAGGACCTG CTGCGGCTCA AGGATTATCA GAAGCGCCTG 
GACTTGTCCC TCCTGCGGCA GAGCAGCGAC CCCATGCTAA GCGAGTTCAA GAACCTGGAC 
ATCACCAAAA AGAAGCTGAT CTACGAGGGT CCGCTGACGT GGCGGCTGAC GAAGGACAAG 
GCTGTAGAGG TACACGTGCT GCTGCTGGAT GACCTGCTGC TGCTGCTCCA GCGCCAGGAC 
GATCGGCTGC TGCTCAAGTC CCACAGCCGG ACGCTGACAC CCACACCCGA CGGCAAGACC 
ATGCTGCGGC CGGTGCTGCG GCTCACCTCC GCCATAACCC GCGAGGTGGC TACCGATCAC 
AAAGCCTTCT ATGTCCTTTT TACCTGGGAC CAGGAGGCCC AGATATATGA GCTGGTGGCC 
CAGACGGTGT CGGAACGGAA GAACTGGTGT TCCCTCATCA CTGAAACTGC TGGATCCTTG 
AAGGTCCCTG CCCCCGCCTC TCGCCCCAAA CCCCGGCCCA GCCCAAGCAG CACCCGAGAA 
CCCCTGCTCA GCAGCTCAGA GAATGGCAAC GGTGGTGGCC GAGAGATGCC CCCAGCTGAC 
GGCCAGACGG AGAGAATCCT CAGCACCCTC CTGCCCTACT GCAGGCCCCG CCCTGAGGGC 
CAGCTTGCTG CCAAGGCCCT TGAGAAAGTG CTGTCCCTGA AGCAGCTCCT GTTTCCCTCG 
GAGGAAGACA GTGGGGCAGG GCCTCCCCAG GAAGGGGATG GGGTCCCTGG GGGTGGCCCC 
CTGAACCCGA CACAGACCCA CACCCAGGAA ATTCGGGAAA AGCTGCTCAG CCTGGAGGAG 
ACCATGAAAC AGCTGGAGGA GGTGGAGGAG GAATTTTGTC ACCTGCGACT CCTCCTGTCT 
CAGCTTGGGG AGAACAGCAC CCCCCAGCCT GGGTGTACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967638.1
CDS	475..2976
Translation	MLSSLGPKEAKKAFLDFCHCFLDKTAVLRVLVPPAVAFELDRTRPEVISEDLQRQFVQEVVQSQQAAVSRLLEDFRSKRLMGMTPWEQDLTQLEAWVGRDRASFEARERHLAERLLAHLEEMQHTISNDEEKSAAVVNAISLYMRHLGVRTKSGDKKSGMNFFRKKVMGNRRSDEPTKTKKGLSIFLDPARWNRGEPQASDFRHLKVETDAEKPGLTERKGGLGVPSRDRNVGAPGQDVPGVSLHPLPGDSPDREPGVDTPPELGDPPPQGPASLEPPGPSESTEEGPDTESPEPGDEGDPGRSGLELEPEEPPGWRELIPPGTLHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDLFYQPMLEGNFFSTEDLQNIFPSLDELIEVHSLFLDRLMKRRQDSGCLIEEIGDVLLARFDGAEGKWFQKISSRFCSRQSFALEQLKAKQRKDPRFCAFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIGQNTEESAEREKVELAAECCREILHHVNQAVRDMEDLLRLKDYQKRLDLSLLRQSSDPMLSEFKNLDITKKKLIYEGPLTWRLTKDKAVEVHVLLLDDLLLLLQRQDDRLLLKSHSRTLTPTPDGKTMLRPVLRLTSAITREVATDHKAFYVLFTWDQEAQIYELVAQTVSERKNWCSLITETAGSLKVPAPASRPKPRPSPSSTREPLLSSSENGNGGGREMPPADGQTERILSTLLPYCRPRPEGQLAAKALEKVLSLKQLLFPSEEDSGAGPPQEGDGVPGGGPLNPTQTHTQEIREKLLSLEETMKQLEEVEEEFCHLRLLLSQLGENSTPQPGCT

Target ORF information:

RefSeq Version	XM_014112163.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris rho guanine nucleotide exchange factor 1-like (LOC100856005), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014112163.2

ORF Insert Sequence:

ATGCTGAGTT CCCTGGGCCC CAAGGAGGCC AAGAAGGCCT TCCTCGACTT CTGCCATTGC 
TTCCTGGACA AGACGGCGGT TTTGCGGGTG CTAGTCCCTC CCGCCGTCGC CTTTGAACTT 
GACCGCACGC GGCCCGAGGT CATCTCCGAG GACTTGCAGC GGCAGTTTGT ACAGGAGGTA 
GTGCAGAGCC AGCAGGCAGC TGTTAGCCGG CTGCTGGAGG ACTTCCGCTC CAAGCGGCTC 
ATGGGCATGA CACCCTGGGA GCAGGACCTG ACCCAGCTGG AGGCCTGGGT TGGGCGGGAC 
CGTGCCAGCT TTGAGGCCCG GGAGCGGCAC CTGGCTGAAC GGCTGCTGGC CCACCTGGAG 
GAGATGCAAC ACACCATCTC TAACGATGAG GAAAAGAGTG CCGCTGTGGT CAATGCCATC 
AGCCTGTACA TGCGCCACCT TGGGGTGCGG ACCAAGAGCG GAGACAAGAA GTCAGGGATG 
AATTTCTTCC GCAAAAAGGT GATGGGGAAT CGGCGGTCAG ATGAGCCAAC CAAGACCAAA 
AAAGGCCTGA GCATCTTCCT AGACCCTGCT CGCTGGAACC GGGGAGAGCC TCAGGCCTCG 
GATTTCCGAC ACCTCAAAGT CGAGACTGAT GCTGAGAAAC CAGGCCTTAC GGAAAGGAAG 
GGAGGCCTGG GGGTGCCCTC CCGGGACCGG AATGTTGGGG CTCCTGGGCA GGATGTCCCT 
GGAGTTTCTC TGCACCCTCT GCCCGGGGAC AGCCCTGACC GGGAACCAGG TGTTGACACC 
CCGCCAGAGC TGGGGGACCC ACCCCCCCAG GGCCCGGCCA GCCTGGAGCC CCCGGGGCCC 
TCGGAGAGCA CTGAGGAGGG TCCTGACACA GAGAGCCCTG AGCCTGGAGA CGAAGGGGAC 
CCTGGGCGGT CAGGACTAGA GCTGGAACCA GAAGAGCCCC CTGGCTGGCG GGAGCTCATC 
CCCCCAGGCA CCCTGCACAG CCTGCCTAAG AGCCAGGTGA AGCGGCAGGA GGTCATCAGC 
GAGCTACTGG TGACAGAGGC TGCCCATGTG CGCATGCTCC GGGTGCTGCA TGACCTCTTC 
TACCAGCCCA TGTTGGAAGG GAACTTCTTC TCCACGGAGG ATCTACAGAA CATCTTCCCC 
AGCCTGGATG AGCTCATCGA GGTGCATTCC CTGTTCCTCG ATCGACTGAT GAAGCGGAGG 
CAGGATAGTG GCTGCCTCAT TGAGGAGATC GGAGATGTAC TGCTGGCCCG GTTTGATGGT 
GCCGAAGGCA AATGGTTCCA GAAAATCTCC TCCCGCTTCT GCAGCCGCCA GTCATTTGCC 
TTAGAGCAGC TCAAAGCCAA GCAGCGCAAG GATCCTCGGT TCTGTGCCTT TGTGCAGGAG 
GCCGAGAGCC GCCCCCGGTG CCGCCGCCTG CAGCTGAAGG ATATGATCCC CACGGAGATG 
CAGCGTCTGA CCAAGTACCC CCTGCTCCTG CAGAGCATTG GGCAGAACAC AGAAGAGTCA 
GCAGAACGGG AGAAAGTAGA ACTGGCAGCT GAGTGCTGCC GGGAAATTCT GCACCATGTT 
AACCAAGCCG TGCGTGACAT GGAGGACCTG CTGCGGCTCA AGGATTATCA GAAGCGCCTG 
GACTTGTCCC TCCTGCGGCA GAGCAGCGAC CCCATGCTAA GCGAGTTCAA GAACCTGGAC 
ATCACCAAAA AGAAGCTGAT CTACGAGGGT CCGCTGACGT GGCGGCTGAC GAAGGACAAG 
GCTGTAGAGG TACACGTGCT GCTGCTGGAT GACCTGCTGC TGCTGCTCCA GCGCCAGGAC 
GATCGGCTGC TGCTCAAGTC CCACAGCCGG ACGCTGACAC CCACACCCGA CGGCAAGACC 
ATGCTGCGGC CGGTGCTGCG GCTCACCTCC GCCATAACCC GCGAGGTGGC TACCGATCAC 
AAAGCCTTCT ATGTCCTTTT TACCTGGGAC CAGGAGGCCC AGATATATGA GCTGGTGGCC 
CAGACGGTGT CGGAACGGAA GAACTGGTGT TCCCTCATCA CTGAAACTGC TGGATCCTTG 
AAGGTCCCTG CCCCCGCCTC TCGCCCCAAA CCCCGGCCCA GCCCAAGCAG CACCCGAGAA 
CCCCTGCTCA GCAGCTCAGA GAATGGCAAC GGTGGTGGCC GAGAGATGCC CCCAGCTGAC 
GGCCAGACGG AGAGAATCCT CAGCACCCTC CTGCCCTACT GCAGGCCCCG CCCTGAGGGC 
CAGCTTGCTG CCAAGGCCCT TGAGAAAGTG CTGTCCCTGA AGCAGCTCCT GTTTCCCTCG 
GAGGAAGACA GTGGGGCAGG GCCTCCCCAG GAAGGGGATG GGGTCCCTGG GGGTGGCCCC 
CTGAACCCGA CACAGACCCA CACCCAGGAA ATTCGGGAAA AGCTGCTCAG CCTGGAGGAG 
ACCATGAAAC AGCTGGAGGA GGTGGAGGAG GAATTTTGTC ACCTGCGACT CCTCCTGTCT 
CAGCTTGGGG AGAACAGCAC CCCCCAGCCT GGGTGTACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100856005 ( XM_014112163.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013967638.1 Canis lupus familiaris rho guanine nucleotide exchange factor 1-like (LOC100856005), transcript variant X4, mRNA.

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CloneID	OCe45891
Clone ID Related Accession (Same CDS sequence)	XM_014112163.2 , XM_014112163.1
Accession Version	XM_014112163.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2502bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	rho guanine nucleotide exchange factor 1-like isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003727276.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGTT CCCTGGGCCC CAAGGAGGCC AAGAAGGCCT TCCTCGACTT CTGCCATTGC 
TTCCTGGACA AGACGGCGGT TTTGCGGGTG CTAGTCCCTC CCGCCGTCGC CTTTGAACTT 
GACCGCACGC GGCCCGAGGT CATCTCCGAG GACTTGCAGC GGCAGTTTGT ACAGGAGGTA 
GTGCAGAGCC AGCAGGCAGC TGTTAGCCGG CTGCTGGAGG ACTTCCGCTC CAAGCGGCTC 
ATGGGCATGA CACCCTGGGA GCAGGACCTG ACCCAGCTGG AGGCCTGGGT TGGGCGGGAC 
CGTGCCAGCT TTGAGGCCCG GGAGCGGCAC CTGGCTGAAC GGCTGCTGGC CCACCTGGAG 
GAGATGCAAC ACACCATCTC TAACGATGAG GAAAAGAGTG CCGCTGTGGT CAATGCCATC 
AGCCTGTACA TGCGCCACCT TGGGGTGCGG ACCAAGAGCG GAGACAAGAA GTCAGGGATG 
AATTTCTTCC GCAAAAAGGT GATGGGGAAT CGGCGGTCAG ATGAGCCAAC CAAGACCAAA 
AAAGGCCTGA GCATCTTCCT AGACCCTGCT CGCTGGAACC GGGGAGAGCC TCAGGCCTCG 
GATTTCCGAC ACCTCAAAGT CGAGACTGAT GCTGAGAAAC CAGGCCTTAC GGAAAGGAAG 
GGAGGCCTGG GGGTGCCCTC CCGGGACCGG AATGTTGGGG CTCCTGGGCA GGATGTCCCT 
GGAGTTTCTC TGCACCCTCT GCCCGGGGAC AGCCCTGACC GGGAACCAGG TGTTGACACC 
CCGCCAGAGC TGGGGGACCC ACCCCCCCAG GGCCCGGCCA GCCTGGAGCC CCCGGGGCCC 
TCGGAGAGCA CTGAGGAGGG TCCTGACACA GAGAGCCCTG AGCCTGGAGA CGAAGGGGAC 
CCTGGGCGGT CAGGACTAGA GCTGGAACCA GAAGAGCCCC CTGGCTGGCG GGAGCTCATC 
CCCCCAGGCA CCCTGCACAG CCTGCCTAAG AGCCAGGTGA AGCGGCAGGA GGTCATCAGC 
GAGCTACTGG TGACAGAGGC TGCCCATGTG CGCATGCTCC GGGTGCTGCA TGACCTCTTC 
TACCAGCCCA TGTTGGAAGG GAACTTCTTC TCCACGGAGG ATCTACAGAA CATCTTCCCC 
AGCCTGGATG AGCTCATCGA GGTGCATTCC CTGTTCCTCG ATCGACTGAT GAAGCGGAGG 
CAGGATAGTG GCTGCCTCAT TGAGGAGATC GGAGATGTAC TGCTGGCCCG GTTTGATGGT 
GCCGAAGGCA AATGGTTCCA GAAAATCTCC TCCCGCTTCT GCAGCCGCCA GTCATTTGCC 
TTAGAGCAGC TCAAAGCCAA GCAGCGCAAG GATCCTCGGT TCTGTGCCTT TGTGCAGGAG 
GCCGAGAGCC GCCCCCGGTG CCGCCGCCTG CAGCTGAAGG ATATGATCCC CACGGAGATG 
CAGCGTCTGA CCAAGTACCC CCTGCTCCTG CAGAGCATTG GGCAGAACAC AGAAGAGTCA 
GCAGAACGGG AGAAAGTAGA ACTGGCAGCT GAGTGCTGCC GGGAAATTCT GCACCATGTT 
AACCAAGCCG TGCGTGACAT GGAGGACCTG CTGCGGCTCA AGGATTATCA GAAGCGCCTG 
GACTTGTCCC TCCTGCGGCA GAGCAGCGAC CCCATGCTAA GCGAGTTCAA GAACCTGGAC 
ATCACCAAAA AGAAGCTGAT CTACGAGGGT CCGCTGACGT GGCGGCTGAC GAAGGACAAG 
GCTGTAGAGG TACACGTGCT GCTGCTGGAT GACCTGCTGC TGCTGCTCCA GCGCCAGGAC 
GATCGGCTGC TGCTCAAGTC CCACAGCCGG ACGCTGACAC CCACACCCGA CGGCAAGACC 
ATGCTGCGGC CGGTGCTGCG GCTCACCTCC GCCATAACCC GCGAGGTGGC TACCGATCAC 
AAAGCCTTCT ATGTCCTTTT TACCTGGGAC CAGGAGGCCC AGATATATGA GCTGGTGGCC 
CAGACGGTGT CGGAACGGAA GAACTGGTGT TCCCTCATCA CTGAAACTGC TGGATCCTTG 
AAGGTCCCTG CCCCCGCCTC TCGCCCCAAA CCCCGGCCCA GCCCAAGCAG CACCCGAGAA 
CCCCTGCTCA GCAGCTCAGA GAATGGCAAC GGTGGTGGCC GAGAGATGCC CCCAGCTGAC 
GGCCAGACGG AGAGAATCCT CAGCACCCTC CTGCCCTACT GCAGGCCCCG CCCTGAGGGC 
CAGCTTGCTG CCAAGGCCCT TGAGAAAGTG CTGTCCCTGA AGCAGCTCCT GTTTCCCTCG 
GAGGAAGACA GTGGGGCAGG GCCTCCCCAG GAAGGGGATG GGGTCCCTGG GGGTGGCCCC 
CTGAACCCGA CACAGACCCA CACCCAGGAA ATTCGGGAAA AGCTGCTCAG CCTGGAGGAG 
ACCATGAAAC AGCTGGAGGA GGTGGAGGAG GAATTTTGTC ACCTGCGACT CCTCCTGTCT 
CAGCTTGGGG AGAACAGCAC CCCCCAGCCT GGGTGTACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967638.1
CDS	454..2955
Translation	MLSSLGPKEAKKAFLDFCHCFLDKTAVLRVLVPPAVAFELDRTRPEVISEDLQRQFVQEVVQSQQAAVSRLLEDFRSKRLMGMTPWEQDLTQLEAWVGRDRASFEARERHLAERLLAHLEEMQHTISNDEEKSAAVVNAISLYMRHLGVRTKSGDKKSGMNFFRKKVMGNRRSDEPTKTKKGLSIFLDPARWNRGEPQASDFRHLKVETDAEKPGLTERKGGLGVPSRDRNVGAPGQDVPGVSLHPLPGDSPDREPGVDTPPELGDPPPQGPASLEPPGPSESTEEGPDTESPEPGDEGDPGRSGLELEPEEPPGWRELIPPGTLHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDLFYQPMLEGNFFSTEDLQNIFPSLDELIEVHSLFLDRLMKRRQDSGCLIEEIGDVLLARFDGAEGKWFQKISSRFCSRQSFALEQLKAKQRKDPRFCAFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIGQNTEESAEREKVELAAECCREILHHVNQAVRDMEDLLRLKDYQKRLDLSLLRQSSDPMLSEFKNLDITKKKLIYEGPLTWRLTKDKAVEVHVLLLDDLLLLLQRQDDRLLLKSHSRTLTPTPDGKTMLRPVLRLTSAITREVATDHKAFYVLFTWDQEAQIYELVAQTVSERKNWCSLITETAGSLKVPAPASRPKPRPSPSSTREPLLSSSENGNGGGREMPPADGQTERILSTLLPYCRPRPEGQLAAKALEKVLSLKQLLFPSEEDSGAGPPQEGDGVPGGGPLNPTQTHTQEIREKLLSLEETMKQLEEVEEEFCHLRLLLSQLGENSTPQPGCT

Target ORF information:

RefSeq Version	XM_014112163.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris rho guanine nucleotide exchange factor 1-like (LOC100856005), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014112163.1

ORF Insert Sequence:

ATGCTGAGTT CCCTGGGCCC CAAGGAGGCC AAGAAGGCCT TCCTCGACTT CTGCCATTGC 
TTCCTGGACA AGACGGCGGT TTTGCGGGTG CTAGTCCCTC CCGCCGTCGC CTTTGAACTT 
GACCGCACGC GGCCCGAGGT CATCTCCGAG GACTTGCAGC GGCAGTTTGT ACAGGAGGTA 
GTGCAGAGCC AGCAGGCAGC TGTTAGCCGG CTGCTGGAGG ACTTCCGCTC CAAGCGGCTC 
ATGGGCATGA CACCCTGGGA GCAGGACCTG ACCCAGCTGG AGGCCTGGGT TGGGCGGGAC 
CGTGCCAGCT TTGAGGCCCG GGAGCGGCAC CTGGCTGAAC GGCTGCTGGC CCACCTGGAG 
GAGATGCAAC ACACCATCTC TAACGATGAG GAAAAGAGTG CCGCTGTGGT CAATGCCATC 
AGCCTGTACA TGCGCCACCT TGGGGTGCGG ACCAAGAGCG GAGACAAGAA GTCAGGGATG 
AATTTCTTCC GCAAAAAGGT GATGGGGAAT CGGCGGTCAG ATGAGCCAAC CAAGACCAAA 
AAAGGCCTGA GCATCTTCCT AGACCCTGCT CGCTGGAACC GGGGAGAGCC TCAGGCCTCG 
GATTTCCGAC ACCTCAAAGT CGAGACTGAT GCTGAGAAAC CAGGCCTTAC GGAAAGGAAG 
GGAGGCCTGG GGGTGCCCTC CCGGGACCGG AATGTTGGGG CTCCTGGGCA GGATGTCCCT 
GGAGTTTCTC TGCACCCTCT GCCCGGGGAC AGCCCTGACC GGGAACCAGG TGTTGACACC 
CCGCCAGAGC TGGGGGACCC ACCCCCCCAG GGCCCGGCCA GCCTGGAGCC CCCGGGGCCC 
TCGGAGAGCA CTGAGGAGGG TCCTGACACA GAGAGCCCTG AGCCTGGAGA CGAAGGGGAC 
CCTGGGCGGT CAGGACTAGA GCTGGAACCA GAAGAGCCCC CTGGCTGGCG GGAGCTCATC 
CCCCCAGGCA CCCTGCACAG CCTGCCTAAG AGCCAGGTGA AGCGGCAGGA GGTCATCAGC 
GAGCTACTGG TGACAGAGGC TGCCCATGTG CGCATGCTCC GGGTGCTGCA TGACCTCTTC 
TACCAGCCCA TGTTGGAAGG GAACTTCTTC TCCACGGAGG ATCTACAGAA CATCTTCCCC 
AGCCTGGATG AGCTCATCGA GGTGCATTCC CTGTTCCTCG ATCGACTGAT GAAGCGGAGG 
CAGGATAGTG GCTGCCTCAT TGAGGAGATC GGAGATGTAC TGCTGGCCCG GTTTGATGGT 
GCCGAAGGCA AATGGTTCCA GAAAATCTCC TCCCGCTTCT GCAGCCGCCA GTCATTTGCC 
TTAGAGCAGC TCAAAGCCAA GCAGCGCAAG GATCCTCGGT TCTGTGCCTT TGTGCAGGAG 
GCCGAGAGCC GCCCCCGGTG CCGCCGCCTG CAGCTGAAGG ATATGATCCC CACGGAGATG 
CAGCGTCTGA CCAAGTACCC CCTGCTCCTG CAGAGCATTG GGCAGAACAC AGAAGAGTCA 
GCAGAACGGG AGAAAGTAGA ACTGGCAGCT GAGTGCTGCC GGGAAATTCT GCACCATGTT 
AACCAAGCCG TGCGTGACAT GGAGGACCTG CTGCGGCTCA AGGATTATCA GAAGCGCCTG 
GACTTGTCCC TCCTGCGGCA GAGCAGCGAC CCCATGCTAA GCGAGTTCAA GAACCTGGAC 
ATCACCAAAA AGAAGCTGAT CTACGAGGGT CCGCTGACGT GGCGGCTGAC GAAGGACAAG 
GCTGTAGAGG TACACGTGCT GCTGCTGGAT GACCTGCTGC TGCTGCTCCA GCGCCAGGAC 
GATCGGCTGC TGCTCAAGTC CCACAGCCGG ACGCTGACAC CCACACCCGA CGGCAAGACC 
ATGCTGCGGC CGGTGCTGCG GCTCACCTCC GCCATAACCC GCGAGGTGGC TACCGATCAC 
AAAGCCTTCT ATGTCCTTTT TACCTGGGAC CAGGAGGCCC AGATATATGA GCTGGTGGCC 
CAGACGGTGT CGGAACGGAA GAACTGGTGT TCCCTCATCA CTGAAACTGC TGGATCCTTG 
AAGGTCCCTG CCCCCGCCTC TCGCCCCAAA CCCCGGCCCA GCCCAAGCAG CACCCGAGAA 
CCCCTGCTCA GCAGCTCAGA GAATGGCAAC GGTGGTGGCC GAGAGATGCC CCCAGCTGAC 
GGCCAGACGG AGAGAATCCT CAGCACCCTC CTGCCCTACT GCAGGCCCCG CCCTGAGGGC 
CAGCTTGCTG CCAAGGCCCT TGAGAAAGTG CTGTCCCTGA AGCAGCTCCT GTTTCCCTCG 
GAGGAAGACA GTGGGGCAGG GCCTCCCCAG GAAGGGGATG GGGTCCCTGG GGGTGGCCCC 
CTGAACCCGA CACAGACCCA CACCCAGGAA ATTCGGGAAA AGCTGCTCAG CCTGGAGGAG 
ACCATGAAAC AGCTGGAGGA GGTGGAGGAG GAATTTTGTC ACCTGCGACT CCTCCTGTCT 
CAGCTTGGGG AGAACAGCAC CCCCCAGCCT GGGTGTACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC100856005 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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