SEMA6D cDNA ORF clone, Canis lupus familiaris(dog)

The following SEMA6D gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEMA6D cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe28820 XM_003640052.4
Latest version!
Canis lupus familiaris semaphorin 6D (SEMA6D), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe28813 XM_005638381.3
Latest version!
Canis lupus familiaris semaphorin 6D (SEMA6D), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe28813 XM_005638379.3
Latest version!
Canis lupus familiaris semaphorin 6D (SEMA6D), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe28813 XM_005638382.2
Latest version!
Canis lupus familiaris sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (SEMA6D), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe28813 XM_022413042.1
Latest version!
Canis lupus familiaris semaphorin 6D (SEMA6D), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe28814 XM_003640050.4
Latest version!
Canis lupus familiaris semaphorin 6D (SEMA6D), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe28815 XM_005638383.3
Latest version!
Canis lupus familiaris semaphorin 6D (SEMA6D), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe28816 XM_005638384.3
Latest version!
Canis lupus familiaris semaphorin 6D (SEMA6D), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe28817 XM_005638385.3
Latest version!
Canis lupus familiaris semaphorin 6D (SEMA6D), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe28818 XM_003640053.4
Latest version!
Canis lupus familiaris semaphorin 6D (SEMA6D), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe28819 XM_003640051.4
Latest version!
Canis lupus familiaris semaphorin 6D (SEMA6D), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OCe28813 XM_005638379.2 Canis lupus familiaris sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (SEMA6D), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe28818 XM_003640053.3 Canis lupus familiaris sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (SEMA6D), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe28817 XM_005638385.2 Canis lupus familiaris sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (SEMA6D), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe28814 XM_003640050.3 Canis lupus familiaris sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (SEMA6D), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe28813 XM_005638381.2 Canis lupus familiaris sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (SEMA6D), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe28815 XM_005638383.2 Canis lupus familiaris sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (SEMA6D), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe28816 XM_005638384.2 Canis lupus familiaris sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (SEMA6D), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe28820 XM_003640052.3 Canis lupus familiaris sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (SEMA6D), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe28819 XM_003640051.3 Canis lupus familiaris sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (SEMA6D), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe28820
    Clone ID Related Accession (Same CDS sequence) XM_003640052.4 , XM_003640052.3
    Accession Version XM_003640052.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2997bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product semaphorin-6D isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003640052.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGGTTCT TCCTGCTTTG TGCCTACATG CTGCTCCTGA TGATTTCCCA GCTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTC AATACTGTTG ATTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AATGAATCCC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAGTCCCCAA GACAGAAGTA ATACCAAGCA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ACCGAGAAAA CTGTGCTATG AAGGGCAAAC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTCCC AAGAAATGAT GAGATGGTTT TTGTATGTGG TACCAATGCA
    TTCAATCCCA TGTGCAGATA CTATAGGTTG AATACCTTAG AGTATGATGG AGAGGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTAT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC CCTTCGTACA ATAAAATATG ATTCCAAATG GATAAAAGAG
    CCACATTTTC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAATAATTT AGGCAAGGCT GTATATTCCC GTGTGGCCCG CATATGTAAA
    AATGACATGG GTGGCTCGCA GCGGGTCCTG GAGAAACACT GGACTTCATT TCTGAAGGCT
    CGGCTTAACT GCTCTGTCCC CGGAGATTCG TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACGGACATCA TACAAATCAA TGGCATCCCC ACTGTGGTCG GGGTGTTTAC CACACAGCTC
    AACAGCATTC CTGGGTCTGC AGTGTGTGCA TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGCT TTAAAGAACA GAAAACCCCA GATTCTGTTT GGACAGCAGT CCCTGAAGAC
    AAAGTACCCA AGCCAAGGCC TGGCTGTTGT GCAAAGCATG GCCTTGCCGA AGCTTATAAA
    ACCTCCATAG ATTTCCCTGA CGAAACCCTG TCATTCATCA AATCCCATCC GCTAATGGAC
    GCTGCTGTAC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCTGTTGA CCACACTGCT GGGCCCCATC AGAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTGCTTAAA GTTTTGGCAA AGACCAGCCC TTTCTCTTTG
    AATGACAGTG TATTACTGGA AGAGATTGAA GCATACAACC AAGCAAAGTG CAATGCTGAG
    AATGAGGAAG ACAGAAGGGT CATCTCCTTA CAGTTGGATA AAGACCATCA CGCTGTGTAC
    GTGGCATTCT CTAGCTGCGT CATCCGCCTC CCTCTCAGCC GTTGCGAGCG TTACGGATCC
    TGTAAGAAGT CTTGTGTTGC ATCTCGGGAC CCATACTGTG GCTGGTTAAG CCAGGGGGCC
    TGTGGCAGAG TGACCCCAGG GATGCTCGCT GGAGGATATG AACAAGACAC AGAGTACGGC
    AACACGGCCC ACCTAGGGGA CTGCCATGGT GTGCGATGGG AAGTCCAATC GGGAGAGTCC
    AACCAGATGG TCCACATGAA CGTCCTCATC ACCTGTGTCT TTGCCGCTTT TGTTTTGGGT
    GCGTTCATTG CAGGGGTGGC AGTATACTGC TATCGTGACA TGTTTGTTCG GAAAAACAGA
    AAGATTCATA AGGATGCAGA ATCTGCCCAG TCGTGCACAG ACTCCAGTGG AAGTTTTGCC
    AAACTGAATG GTCTGTTTGA TAGCCCGGTC AAGGAATATC AACAGAATAT CGATTCTCCC
    AAATTATACA GTAACCTGCT GACTAGTCGG AAAGAGCTGC CACCCAATGG AGATACCAAA
    TCCATGGTGA TGGACCATCG AGGCCAACCT CCCGAACTGG CTGCTCTCCC CACGCCTGAG
    TCAACTCCTG TGCTTCACCA GAAGACCCTG CAGGCCATGA AGAGCCACTC AGATAAGGCC
    CATGGCCATG GGGCTTCAAG GAAAGAAACC TCACAGTTTT TCCCATCTAG TCCACCACCC
    CATTCCCCAT TAAGTCATGG GCATATCCCC AGTGCCATTG TTCTTCCTAA TGCTACTCAT
    GACTACAACA CGTCTTTCTC AAACTCCAAT GCTCACAAAG CTGAAAAGAA GCTTCAAAAC
    ATTGACCACC CACTTACAAA GTCATCCAGT AAAAGAGATC ACCGGCGTTC TGTGGATTCC
    AGAAATACCC TCAATGATCT CCTGAAGCAT TTAAATGACC CAAACAGCAA CCCCAAAGCC
    ATCATGGGAG ATATCCAGAT GGCCCACCAG ACCCTAATGC TGGATCCTGT GGGACCTATG
    TCTGAGGTCC CGCCCAAGGT CCCTAACCGG GAGGCATCCC TATACTCTCC TCCTTCAACT
    CTCCCCAGAA ATAGCCCAAC CAAGCGAGTG GATGTCCCCA CCACTCCTGG GGTCCCAATG
    ACTTCTCTGG AAAGACAAAG GGGTTACCAC AAAAATTCCT CCCAGAGGCA CTCTATATCT
    GCTATGCCTA AAAACTTAAA CTCACCAAAT GGTGTTTTGT TATCCAGACA GCCTAGCATG
    AACCGTGGTG GGTATGTGCC CACCTCCACT GGGGCAAAGG TGGACTATAT TCAGGGAGCA
    CCAGTGAGTG TTCATCTGCA GCCTTCCCTC TCCAGACAGA GCAGCTATAC CAGTAATGGC
    ACCCTTCCTA GGACGGGACT AAAGAGGACA CCGTCCTTAA AACCTGATGT GCCACCAAAG
    CCTTCATTTG TTCCTCAAAC CACATCTGTC AGACCACTGA ACAAATACAC TTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003640100.1
    CDS287..3283
    Translation

    Target ORF information:

    RefSeq Version XM_003640052.4
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris semaphorin 6D (SEMA6D), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003640052.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGGTTCT TCCTGCTTTG TGCCTACATG CTGCTCCTGA TGATTTCCCA GCTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTC AATACTGTTG ATTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AATGAATCCC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAGTCCCCAA GACAGAAGTA ATACCAAGCA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ACCGAGAAAA CTGTGCTATG AAGGGCAAAC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTCCC AAGAAATGAT GAGATGGTTT TTGTATGTGG TACCAATGCA
    TTCAATCCCA TGTGCAGATA CTATAGGTTG AATACCTTAG AGTATGATGG AGAGGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTAT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC CCTTCGTACA ATAAAATATG ATTCCAAATG GATAAAAGAG
    CCACATTTTC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAATAATTT AGGCAAGGCT GTATATTCCC GTGTGGCCCG CATATGTAAA
    AATGACATGG GTGGCTCGCA GCGGGTCCTG GAGAAACACT GGACTTCATT TCTGAAGGCT
    CGGCTTAACT GCTCTGTCCC CGGAGATTCG TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACGGACATCA TACAAATCAA TGGCATCCCC ACTGTGGTCG GGGTGTTTAC CACACAGCTC
    AACAGCATTC CTGGGTCTGC AGTGTGTGCA TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGCT TTAAAGAACA GAAAACCCCA GATTCTGTTT GGACAGCAGT CCCTGAAGAC
    AAAGTACCCA AGCCAAGGCC TGGCTGTTGT GCAAAGCATG GCCTTGCCGA AGCTTATAAA
    ACCTCCATAG ATTTCCCTGA CGAAACCCTG TCATTCATCA AATCCCATCC GCTAATGGAC
    GCTGCTGTAC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCTGTTGA CCACACTGCT GGGCCCCATC AGAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTGCTTAAA GTTTTGGCAA AGACCAGCCC TTTCTCTTTG
    AATGACAGTG TATTACTGGA AGAGATTGAA GCATACAACC AAGCAAAGTG CAATGCTGAG
    AATGAGGAAG ACAGAAGGGT CATCTCCTTA CAGTTGGATA AAGACCATCA CGCTGTGTAC
    GTGGCATTCT CTAGCTGCGT CATCCGCCTC CCTCTCAGCC GTTGCGAGCG TTACGGATCC
    TGTAAGAAGT CTTGTGTTGC ATCTCGGGAC CCATACTGTG GCTGGTTAAG CCAGGGGGCC
    TGTGGCAGAG TGACCCCAGG GATGCTCGCT GGAGGATATG AACAAGACAC AGAGTACGGC
    AACACGGCCC ACCTAGGGGA CTGCCATGGT GTGCGATGGG AAGTCCAATC GGGAGAGTCC
    AACCAGATGG TCCACATGAA CGTCCTCATC ACCTGTGTCT TTGCCGCTTT TGTTTTGGGT
    GCGTTCATTG CAGGGGTGGC AGTATACTGC TATCGTGACA TGTTTGTTCG GAAAAACAGA
    AAGATTCATA AGGATGCAGA ATCTGCCCAG TCGTGCACAG ACTCCAGTGG AAGTTTTGCC
    AAACTGAATG GTCTGTTTGA TAGCCCGGTC AAGGAATATC AACAGAATAT CGATTCTCCC
    AAATTATACA GTAACCTGCT GACTAGTCGG AAAGAGCTGC CACCCAATGG AGATACCAAA
    TCCATGGTGA TGGACCATCG AGGCCAACCT CCCGAACTGG CTGCTCTCCC CACGCCTGAG
    TCAACTCCTG TGCTTCACCA GAAGACCCTG CAGGCCATGA AGAGCCACTC AGATAAGGCC
    CATGGCCATG GGGCTTCAAG GAAAGAAACC TCACAGTTTT TCCCATCTAG TCCACCACCC
    CATTCCCCAT TAAGTCATGG GCATATCCCC AGTGCCATTG TTCTTCCTAA TGCTACTCAT
    GACTACAACA CGTCTTTCTC AAACTCCAAT GCTCACAAAG CTGAAAAGAA GCTTCAAAAC
    ATTGACCACC CACTTACAAA GTCATCCAGT AAAAGAGATC ACCGGCGTTC TGTGGATTCC
    AGAAATACCC TCAATGATCT CCTGAAGCAT TTAAATGACC CAAACAGCAA CCCCAAAGCC
    ATCATGGGAG ATATCCAGAT GGCCCACCAG ACCCTAATGC TGGATCCTGT GGGACCTATG
    TCTGAGGTCC CGCCCAAGGT CCCTAACCGG GAGGCATCCC TATACTCTCC TCCTTCAACT
    CTCCCCAGAA ATAGCCCAAC CAAGCGAGTG GATGTCCCCA CCACTCCTGG GGTCCCAATG
    ACTTCTCTGG AAAGACAAAG GGGTTACCAC AAAAATTCCT CCCAGAGGCA CTCTATATCT
    GCTATGCCTA AAAACTTAAA CTCACCAAAT GGTGTTTTGT TATCCAGACA GCCTAGCATG
    AACCGTGGTG GGTATGTGCC CACCTCCACT GGGGCAAAGG TGGACTATAT TCAGGGAGCA
    CCAGTGAGTG TTCATCTGCA GCCTTCCCTC TCCAGACAGA GCAGCTATAC CAGTAATGGC
    ACCCTTCCTA GGACGGGACT AAAGAGGACA CCGTCCTTAA AACCTGATGT GCCACCAAAG
    CCTTCATTTG TTCCTCAAAC CACATCTGTC AGACCACTGA ACAAATACAC TTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe28820
    Clone ID Related Accession (Same CDS sequence) XM_003640052.4 , XM_003640052.3
    Accession Version XM_003640052.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2997bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product semaphorin-6D isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003640052.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGGTTCT TCCTGCTTTG TGCCTACATG CTGCTCCTGA TGATTTCCCA GCTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTC AATACTGTTG ATTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AATGAATCCC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAGTCCCCAA GACAGAAGTA ATACCAAGCA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ACCGAGAAAA CTGTGCTATG AAGGGCAAAC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTCCC AAGAAATGAT GAGATGGTTT TTGTATGTGG TACCAATGCA
    TTCAATCCCA TGTGCAGATA CTATAGGTTG AATACCTTAG AGTATGATGG AGAGGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTAT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC CCTTCGTACA ATAAAATATG ATTCCAAATG GATAAAAGAG
    CCACATTTTC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAATAATTT AGGCAAGGCT GTATATTCCC GTGTGGCCCG CATATGTAAA
    AATGACATGG GTGGCTCGCA GCGGGTCCTG GAGAAACACT GGACTTCATT TCTGAAGGCT
    CGGCTTAACT GCTCTGTCCC CGGAGATTCG TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACGGACATCA TACAAATCAA TGGCATCCCC ACTGTGGTCG GGGTGTTTAC CACACAGCTC
    AACAGCATTC CTGGGTCTGC AGTGTGTGCA TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGCT TTAAAGAACA GAAAACCCCA GATTCTGTTT GGACAGCAGT CCCTGAAGAC
    AAAGTACCCA AGCCAAGGCC TGGCTGTTGT GCAAAGCATG GCCTTGCCGA AGCTTATAAA
    ACCTCCATAG ATTTCCCTGA CGAAACCCTG TCATTCATCA AATCCCATCC GCTAATGGAC
    GCTGCTGTAC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCTGTTGA CCACACTGCT GGGCCCCATC AGAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTGCTTAAA GTTTTGGCAA AGACCAGCCC TTTCTCTTTG
    AATGACAGTG TATTACTGGA AGAGATTGAA GCATACAACC AAGCAAAGTG CAATGCTGAG
    AATGAGGAAG ACAGAAGGGT CATCTCCTTA CAGTTGGATA AAGACCATCA CGCTGTGTAC
    GTGGCATTCT CTAGCTGCGT CATCCGCCTC CCTCTCAGCC GTTGCGAGCG TTACGGATCC
    TGTAAGAAGT CTTGTGTTGC ATCTCGGGAC CCATACTGTG GCTGGTTAAG CCAGGGGGCC
    TGTGGCAGAG TGACCCCAGG GATGCTCGCT GGAGGATATG AACAAGACAC AGAGTACGGC
    AACACGGCCC ACCTAGGGGA CTGCCATGGT GTGCGATGGG AAGTCCAATC GGGAGAGTCC
    AACCAGATGG TCCACATGAA CGTCCTCATC ACCTGTGTCT TTGCCGCTTT TGTTTTGGGT
    GCGTTCATTG CAGGGGTGGC AGTATACTGC TATCGTGACA TGTTTGTTCG GAAAAACAGA
    AAGATTCATA AGGATGCAGA ATCTGCCCAG TCGTGCACAG ACTCCAGTGG AAGTTTTGCC
    AAACTGAATG GTCTGTTTGA TAGCCCGGTC AAGGAATATC AACAGAATAT CGATTCTCCC
    AAATTATACA GTAACCTGCT GACTAGTCGG AAAGAGCTGC CACCCAATGG AGATACCAAA
    TCCATGGTGA TGGACCATCG AGGCCAACCT CCCGAACTGG CTGCTCTCCC CACGCCTGAG
    TCAACTCCTG TGCTTCACCA GAAGACCCTG CAGGCCATGA AGAGCCACTC AGATAAGGCC
    CATGGCCATG GGGCTTCAAG GAAAGAAACC TCACAGTTTT TCCCATCTAG TCCACCACCC
    CATTCCCCAT TAAGTCATGG GCATATCCCC AGTGCCATTG TTCTTCCTAA TGCTACTCAT
    GACTACAACA CGTCTTTCTC AAACTCCAAT GCTCACAAAG CTGAAAAGAA GCTTCAAAAC
    ATTGACCACC CACTTACAAA GTCATCCAGT AAAAGAGATC ACCGGCGTTC TGTGGATTCC
    AGAAATACCC TCAATGATCT CCTGAAGCAT TTAAATGACC CAAACAGCAA CCCCAAAGCC
    ATCATGGGAG ATATCCAGAT GGCCCACCAG ACCCTAATGC TGGATCCTGT GGGACCTATG
    TCTGAGGTCC CGCCCAAGGT CCCTAACCGG GAGGCATCCC TATACTCTCC TCCTTCAACT
    CTCCCCAGAA ATAGCCCAAC CAAGCGAGTG GATGTCCCCA CCACTCCTGG GGTCCCAATG
    ACTTCTCTGG AAAGACAAAG GGGTTACCAC AAAAATTCCT CCCAGAGGCA CTCTATATCT
    GCTATGCCTA AAAACTTAAA CTCACCAAAT GGTGTTTTGT TATCCAGACA GCCTAGCATG
    AACCGTGGTG GGTATGTGCC CACCTCCACT GGGGCAAAGG TGGACTATAT TCAGGGAGCA
    CCAGTGAGTG TTCATCTGCA GCCTTCCCTC TCCAGACAGA GCAGCTATAC CAGTAATGGC
    ACCCTTCCTA GGACGGGACT AAAGAGGACA CCGTCCTTAA AACCTGATGT GCCACCAAAG
    CCTTCATTTG TTCCTCAAAC CACATCTGTC AGACCACTGA ACAAATACAC TTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003640100.1
    CDS314..3310
    Translation

    Target ORF information:

    RefSeq Version XM_003640052.3
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (SEMA6D), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003640052.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGGTTCT TCCTGCTTTG TGCCTACATG CTGCTCCTGA TGATTTCCCA GCTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTC AATACTGTTG ATTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AATGAATCCC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAGTCCCCAA GACAGAAGTA ATACCAAGCA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ACCGAGAAAA CTGTGCTATG AAGGGCAAAC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTCCC AAGAAATGAT GAGATGGTTT TTGTATGTGG TACCAATGCA
    TTCAATCCCA TGTGCAGATA CTATAGGTTG AATACCTTAG AGTATGATGG AGAGGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTAT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC CCTTCGTACA ATAAAATATG ATTCCAAATG GATAAAAGAG
    CCACATTTTC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAATAATTT AGGCAAGGCT GTATATTCCC GTGTGGCCCG CATATGTAAA
    AATGACATGG GTGGCTCGCA GCGGGTCCTG GAGAAACACT GGACTTCATT TCTGAAGGCT
    CGGCTTAACT GCTCTGTCCC CGGAGATTCG TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACGGACATCA TACAAATCAA TGGCATCCCC ACTGTGGTCG GGGTGTTTAC CACACAGCTC
    AACAGCATTC CTGGGTCTGC AGTGTGTGCA TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGCT TTAAAGAACA GAAAACCCCA GATTCTGTTT GGACAGCAGT CCCTGAAGAC
    AAAGTACCCA AGCCAAGGCC TGGCTGTTGT GCAAAGCATG GCCTTGCCGA AGCTTATAAA
    ACCTCCATAG ATTTCCCTGA CGAAACCCTG TCATTCATCA AATCCCATCC GCTAATGGAC
    GCTGCTGTAC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCTGTTGA CCACACTGCT GGGCCCCATC AGAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTGCTTAAA GTTTTGGCAA AGACCAGCCC TTTCTCTTTG
    AATGACAGTG TATTACTGGA AGAGATTGAA GCATACAACC AAGCAAAGTG CAATGCTGAG
    AATGAGGAAG ACAGAAGGGT CATCTCCTTA CAGTTGGATA AAGACCATCA CGCTGTGTAC
    GTGGCATTCT CTAGCTGCGT CATCCGCCTC CCTCTCAGCC GTTGCGAGCG TTACGGATCC
    TGTAAGAAGT CTTGTGTTGC ATCTCGGGAC CCATACTGTG GCTGGTTAAG CCAGGGGGCC
    TGTGGCAGAG TGACCCCAGG GATGCTCGCT GGAGGATATG AACAAGACAC AGAGTACGGC
    AACACGGCCC ACCTAGGGGA CTGCCATGGT GTGCGATGGG AAGTCCAATC GGGAGAGTCC
    AACCAGATGG TCCACATGAA CGTCCTCATC ACCTGTGTCT TTGCCGCTTT TGTTTTGGGT
    GCGTTCATTG CAGGGGTGGC AGTATACTGC TATCGTGACA TGTTTGTTCG GAAAAACAGA
    AAGATTCATA AGGATGCAGA ATCTGCCCAG TCGTGCACAG ACTCCAGTGG AAGTTTTGCC
    AAACTGAATG GTCTGTTTGA TAGCCCGGTC AAGGAATATC AACAGAATAT CGATTCTCCC
    AAATTATACA GTAACCTGCT GACTAGTCGG AAAGAGCTGC CACCCAATGG AGATACCAAA
    TCCATGGTGA TGGACCATCG AGGCCAACCT CCCGAACTGG CTGCTCTCCC CACGCCTGAG
    TCAACTCCTG TGCTTCACCA GAAGACCCTG CAGGCCATGA AGAGCCACTC AGATAAGGCC
    CATGGCCATG GGGCTTCAAG GAAAGAAACC TCACAGTTTT TCCCATCTAG TCCACCACCC
    CATTCCCCAT TAAGTCATGG GCATATCCCC AGTGCCATTG TTCTTCCTAA TGCTACTCAT
    GACTACAACA CGTCTTTCTC AAACTCCAAT GCTCACAAAG CTGAAAAGAA GCTTCAAAAC
    ATTGACCACC CACTTACAAA GTCATCCAGT AAAAGAGATC ACCGGCGTTC TGTGGATTCC
    AGAAATACCC TCAATGATCT CCTGAAGCAT TTAAATGACC CAAACAGCAA CCCCAAAGCC
    ATCATGGGAG ATATCCAGAT GGCCCACCAG ACCCTAATGC TGGATCCTGT GGGACCTATG
    TCTGAGGTCC CGCCCAAGGT CCCTAACCGG GAGGCATCCC TATACTCTCC TCCTTCAACT
    CTCCCCAGAA ATAGCCCAAC CAAGCGAGTG GATGTCCCCA CCACTCCTGG GGTCCCAATG
    ACTTCTCTGG AAAGACAAAG GGGTTACCAC AAAAATTCCT CCCAGAGGCA CTCTATATCT
    GCTATGCCTA AAAACTTAAA CTCACCAAAT GGTGTTTTGT TATCCAGACA GCCTAGCATG
    AACCGTGGTG GGTATGTGCC CACCTCCACT GGGGCAAAGG TGGACTATAT TCAGGGAGCA
    CCAGTGAGTG TTCATCTGCA GCCTTCCCTC TCCAGACAGA GCAGCTATAC CAGTAATGGC
    ACCCTTCCTA GGACGGGACT AAAGAGGACA CCGTCCTTAA AACCTGATGT GCCACCAAAG
    CCTTCATTTG TTCCTCAAAC CACATCTGTC AGACCACTGA ACAAATACAC TTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe28813
    Clone ID Related Accession (Same CDS sequence) XM_005638379.2 , XM_005638382.2 , XM_005638379.3 , XM_005638381.2 , XM_005638381.3 , XM_022413042.1
    Accession Version XM_005638379.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product semaphorin-6D isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005638379.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGGTTCT TCCTGCTTTG TGCCTACATG CTGCTCCTGA TGATTTCCCA GCTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTC AATACTGTTG ATTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AATGAATCCC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAGTCCCCAA GACAGAAGTA ATACCAAGCA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ACCGAGAAAA CTGTGCTATG AAGGGCAAAC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTCCC AAGAAATGAT GAGATGGTTT TTGTATGTGG TACCAATGCA
    TTCAATCCCA TGTGCAGATA CTATAGGTTG AATACCTTAG AGTATGATGG AGAGGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTAT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC CCTTCGTACA ATAAAATATG ATTCCAAATG GATAAAAGAG
    CCACATTTTC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAATAATTT AGGCAAGGCT GTATATTCCC GTGTGGCCCG CATATGTAAA
    AATGACATGG GTGGCTCGCA GCGGGTCCTG GAGAAACACT GGACTTCATT TCTGAAGGCT
    CGGCTTAACT GCTCTGTCCC CGGAGATTCG TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACGGACATCA TACAAATCAA TGGCATCCCC ACTGTGGTCG GGGTGTTTAC CACACAGCTC
    AACAGCATTC CTGGGTCTGC AGTGTGTGCA TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGCT TTAAAGAACA GAAAACCCCA GATTCTGTTT GGACAGCAGT CCCTGAAGAC
    AAAGTACCCA AGCCAAGGCC TGGCTGTTGT GCAAAGCATG GCCTTGCCGA AGCTTATAAA
    ACCTCCATAG ATTTCCCTGA CGAAACCCTG TCATTCATCA AATCCCATCC GCTAATGGAC
    GCTGCTGTAC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCTGTTGA CCACACTGCT GGGCCCCATC AGAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTGCTTAAA GTTTTGGCAA AGACCAGCCC TTTCTCTTTG
    AATGACAGTG TATTACTGGA AGAGATTGAA GCATACAACC AAGCAAAGTG CAATGCTGAG
    AATGAGGAAG ACAGAAGGGT CATCTCCTTA CAGTTGGATA AAGACCATCA CGCTGTGTAC
    GTGGCATTCT CTAGCTGCGT CATCCGCCTC CCTCTCAGCC GTTGCGAGCG TTACGGATCC
    TGTAAGAAGT CTTGTGTTGC ATCTCGGGAC CCATACTGTG GCTGGTTAAG CCAGGGGGCC
    TGTGGCAGAG TGACCCCAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATATGA ACAAGACACA GAGTACGGCA ACACGGCCCA CCTAGGGGAC
    TGCCATGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCTTC ATCATCTGTT ACCACAATGG CAAGTATCCC AGAAATTACA
    CCTAAAGTGA TTGATACCTG GAGGCCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ACACTTCTGA TTTTACTGAT CCTTTATCGG GTATCCCAAA GGGTGTGCGA
    TGGGAAGTCC AATCGGGAGA GTCCAACCAG ATGGTCCACA TGAACGTCCT CATCACCTGT
    GTCTTTGCCG CTTTTGTTTT GGGTGCGTTC ATTGCAGGGG TGGCAGTATA CTGCTATCGT
    GACATGTTTG TTCGGAAAAA CAGAAAGATT CATAAGGATG CAGAATCTGC CCAGTCGTGC
    ACAGACTCCA GTGGAAGTTT TGCCAAACTG AATGGTCTGT TTGATAGCCC GGTCAAGGAA
    TATCAACAGA ATATCGATTC TCCCAAATTA TACAGTAACC TGCTGACTAG TCGGAAAGAG
    CTGCCACCCA ATGGAGATAC CAAATCCATG GTGATGGACC ATCGAGGCCA ACCTCCCGAA
    CTGGCTGCTC TCCCCACGCC TGAGTCAACT CCTGTGCTTC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCAGATAA GGCCCATGGC CATGGGGCTT CAAGGAAAGA AACCTCACAG
    TTTTTCCCAT CTAGTCCACC ACCCCATTCC CCATTAAGTC ATGGGCATAT CCCCAGTGCC
    ATTGTTCTTC CTAATGCTAC TCATGACTAC AACACGTCTT TCTCAAACTC CAATGCTCAC
    AAAGCTGAAA AGAAGCTTCA AAACATTGAC CACCCACTTA CAAAGTCATC CAGTAAAAGA
    GATCACCGGC GTTCTGTGGA TTCCAGAAAT ACCCTCAATG ATCTCCTGAA GCATTTAAAT
    GACCCAAACA GCAACCCCAA AGCCATCATG GGAGATATCC AGATGGCCCA CCAGACCCTA
    ATGCTGGATC CTGTGGGACC TATGTCTGAG GTCCCGCCCA AGGTCCCTAA CCGGGAGGCA
    TCCCTATACT CTCCTCCTTC AACTCTCCCC AGAAATAGCC CAACCAAGCG AGTGGATGTC
    CCCACCACTC CTGGGGTCCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA CCACAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCTGCTATG CCTAAAAACT TAAACTCACC AAATGGTGTT
    TTGTTATCCA GACAGCCTAG CATGAACCGT GGTGGGTATG TGCCCACCTC CACTGGGGCA
    AAGGTGGACT ATATTCAGGG AGCACCAGTG AGTGTTCATC TGCAGCCTTC CCTCTCCAGA
    CAGAGCAGCT ATACCAGTAA TGGCACCCTT CCTAGGACGG GACTAAAGAG GACACCGTCC
    TTAAAACCTG ATGTGCCACC AAAGCCTTCA TTTGTTCCTC AAACCACATC TGTCAGACCA
    CTGAACAAAT ACACTTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005638436.1
    CDS314..3574
    Translation

    Target ORF information:

    RefSeq Version XM_005638379.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (SEMA6D), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005638379.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGGTTCT TCCTGCTTTG TGCCTACATG CTGCTCCTGA TGATTTCCCA GCTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTC AATACTGTTG ATTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AATGAATCCC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAGTCCCCAA GACAGAAGTA ATACCAAGCA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ACCGAGAAAA CTGTGCTATG AAGGGCAAAC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTCCC AAGAAATGAT GAGATGGTTT TTGTATGTGG TACCAATGCA
    TTCAATCCCA TGTGCAGATA CTATAGGTTG AATACCTTAG AGTATGATGG AGAGGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTAT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC CCTTCGTACA ATAAAATATG ATTCCAAATG GATAAAAGAG
    CCACATTTTC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAATAATTT AGGCAAGGCT GTATATTCCC GTGTGGCCCG CATATGTAAA
    AATGACATGG GTGGCTCGCA GCGGGTCCTG GAGAAACACT GGACTTCATT TCTGAAGGCT
    CGGCTTAACT GCTCTGTCCC CGGAGATTCG TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACGGACATCA TACAAATCAA TGGCATCCCC ACTGTGGTCG GGGTGTTTAC CACACAGCTC
    AACAGCATTC CTGGGTCTGC AGTGTGTGCA TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGCT TTAAAGAACA GAAAACCCCA GATTCTGTTT GGACAGCAGT CCCTGAAGAC
    AAAGTACCCA AGCCAAGGCC TGGCTGTTGT GCAAAGCATG GCCTTGCCGA AGCTTATAAA
    ACCTCCATAG ATTTCCCTGA CGAAACCCTG TCATTCATCA AATCCCATCC GCTAATGGAC
    GCTGCTGTAC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCTGTTGA CCACACTGCT GGGCCCCATC AGAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTGCTTAAA GTTTTGGCAA AGACCAGCCC TTTCTCTTTG
    AATGACAGTG TATTACTGGA AGAGATTGAA GCATACAACC AAGCAAAGTG CAATGCTGAG
    AATGAGGAAG ACAGAAGGGT CATCTCCTTA CAGTTGGATA AAGACCATCA CGCTGTGTAC
    GTGGCATTCT CTAGCTGCGT CATCCGCCTC CCTCTCAGCC GTTGCGAGCG TTACGGATCC
    TGTAAGAAGT CTTGTGTTGC ATCTCGGGAC CCATACTGTG GCTGGTTAAG CCAGGGGGCC
    TGTGGCAGAG TGACCCCAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATATGA ACAAGACACA GAGTACGGCA ACACGGCCCA CCTAGGGGAC
    TGCCATGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCTTC ATCATCTGTT ACCACAATGG CAAGTATCCC AGAAATTACA
    CCTAAAGTGA TTGATACCTG GAGGCCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ACACTTCTGA TTTTACTGAT CCTTTATCGG GTATCCCAAA GGGTGTGCGA
    TGGGAAGTCC AATCGGGAGA GTCCAACCAG ATGGTCCACA TGAACGTCCT CATCACCTGT
    GTCTTTGCCG CTTTTGTTTT GGGTGCGTTC ATTGCAGGGG TGGCAGTATA CTGCTATCGT
    GACATGTTTG TTCGGAAAAA CAGAAAGATT CATAAGGATG CAGAATCTGC CCAGTCGTGC
    ACAGACTCCA GTGGAAGTTT TGCCAAACTG AATGGTCTGT TTGATAGCCC GGTCAAGGAA
    TATCAACAGA ATATCGATTC TCCCAAATTA TACAGTAACC TGCTGACTAG TCGGAAAGAG
    CTGCCACCCA ATGGAGATAC CAAATCCATG GTGATGGACC ATCGAGGCCA ACCTCCCGAA
    CTGGCTGCTC TCCCCACGCC TGAGTCAACT CCTGTGCTTC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCAGATAA GGCCCATGGC CATGGGGCTT CAAGGAAAGA AACCTCACAG
    TTTTTCCCAT CTAGTCCACC ACCCCATTCC CCATTAAGTC ATGGGCATAT CCCCAGTGCC
    ATTGTTCTTC CTAATGCTAC TCATGACTAC AACACGTCTT TCTCAAACTC CAATGCTCAC
    AAAGCTGAAA AGAAGCTTCA AAACATTGAC CACCCACTTA CAAAGTCATC CAGTAAAAGA
    GATCACCGGC GTTCTGTGGA TTCCAGAAAT ACCCTCAATG ATCTCCTGAA GCATTTAAAT
    GACCCAAACA GCAACCCCAA AGCCATCATG GGAGATATCC AGATGGCCCA CCAGACCCTA
    ATGCTGGATC CTGTGGGACC TATGTCTGAG GTCCCGCCCA AGGTCCCTAA CCGGGAGGCA
    TCCCTATACT CTCCTCCTTC AACTCTCCCC AGAAATAGCC CAACCAAGCG AGTGGATGTC
    CCCACCACTC CTGGGGTCCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA CCACAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCTGCTATG CCTAAAAACT TAAACTCACC AAATGGTGTT
    TTGTTATCCA GACAGCCTAG CATGAACCGT GGTGGGTATG TGCCCACCTC CACTGGGGCA
    AAGGTGGACT ATATTCAGGG AGCACCAGTG AGTGTTCATC TGCAGCCTTC CCTCTCCAGA
    CAGAGCAGCT ATACCAGTAA TGGCACCCTT CCTAGGACGG GACTAAAGAG GACACCGTCC
    TTAAAACCTG ATGTGCCACC AAAGCCTTCA TTTGTTCCTC AAACCACATC TGTCAGACCA
    CTGAACAAAT ACACTTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe28813
    Clone ID Related Accession (Same CDS sequence) XM_005638379.2 , XM_005638382.2 , XM_005638379.3 , XM_005638381.2 , XM_005638381.3 , XM_022413042.1
    Accession Version XM_005638382.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product semaphorin-6D isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005638382.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGGTTCT TCCTGCTTTG TGCCTACATG CTGCTCCTGA TGATTTCCCA GCTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTC AATACTGTTG ATTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AATGAATCCC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAGTCCCCAA GACAGAAGTA ATACCAAGCA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ACCGAGAAAA CTGTGCTATG AAGGGCAAAC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTCCC AAGAAATGAT GAGATGGTTT TTGTATGTGG TACCAATGCA
    TTCAATCCCA TGTGCAGATA CTATAGGTTG AATACCTTAG AGTATGATGG AGAGGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTAT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC CCTTCGTACA ATAAAATATG ATTCCAAATG GATAAAAGAG
    CCACATTTTC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAATAATTT AGGCAAGGCT GTATATTCCC GTGTGGCCCG CATATGTAAA
    AATGACATGG GTGGCTCGCA GCGGGTCCTG GAGAAACACT GGACTTCATT TCTGAAGGCT
    CGGCTTAACT GCTCTGTCCC CGGAGATTCG TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACGGACATCA TACAAATCAA TGGCATCCCC ACTGTGGTCG GGGTGTTTAC CACACAGCTC
    AACAGCATTC CTGGGTCTGC AGTGTGTGCA TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGCT TTAAAGAACA GAAAACCCCA GATTCTGTTT GGACAGCAGT CCCTGAAGAC
    AAAGTACCCA AGCCAAGGCC TGGCTGTTGT GCAAAGCATG GCCTTGCCGA AGCTTATAAA
    ACCTCCATAG ATTTCCCTGA CGAAACCCTG TCATTCATCA AATCCCATCC GCTAATGGAC
    GCTGCTGTAC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCTGTTGA CCACACTGCT GGGCCCCATC AGAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTGCTTAAA GTTTTGGCAA AGACCAGCCC TTTCTCTTTG
    AATGACAGTG TATTACTGGA AGAGATTGAA GCATACAACC AAGCAAAGTG CAATGCTGAG
    AATGAGGAAG ACAGAAGGGT CATCTCCTTA CAGTTGGATA AAGACCATCA CGCTGTGTAC
    GTGGCATTCT CTAGCTGCGT CATCCGCCTC CCTCTCAGCC GTTGCGAGCG TTACGGATCC
    TGTAAGAAGT CTTGTGTTGC ATCTCGGGAC CCATACTGTG GCTGGTTAAG CCAGGGGGCC
    TGTGGCAGAG TGACCCCAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATATGA ACAAGACACA GAGTACGGCA ACACGGCCCA CCTAGGGGAC
    TGCCATGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCTTC ATCATCTGTT ACCACAATGG CAAGTATCCC AGAAATTACA
    CCTAAAGTGA TTGATACCTG GAGGCCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ACACTTCTGA TTTTACTGAT CCTTTATCGG GTATCCCAAA GGGTGTGCGA
    TGGGAAGTCC AATCGGGAGA GTCCAACCAG ATGGTCCACA TGAACGTCCT CATCACCTGT
    GTCTTTGCCG CTTTTGTTTT GGGTGCGTTC ATTGCAGGGG TGGCAGTATA CTGCTATCGT
    GACATGTTTG TTCGGAAAAA CAGAAAGATT CATAAGGATG CAGAATCTGC CCAGTCGTGC
    ACAGACTCCA GTGGAAGTTT TGCCAAACTG AATGGTCTGT TTGATAGCCC GGTCAAGGAA
    TATCAACAGA ATATCGATTC TCCCAAATTA TACAGTAACC TGCTGACTAG TCGGAAAGAG
    CTGCCACCCA ATGGAGATAC CAAATCCATG GTGATGGACC ATCGAGGCCA ACCTCCCGAA
    CTGGCTGCTC TCCCCACGCC TGAGTCAACT CCTGTGCTTC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCAGATAA GGCCCATGGC CATGGGGCTT CAAGGAAAGA AACCTCACAG
    TTTTTCCCAT CTAGTCCACC ACCCCATTCC CCATTAAGTC ATGGGCATAT CCCCAGTGCC
    ATTGTTCTTC CTAATGCTAC TCATGACTAC AACACGTCTT TCTCAAACTC CAATGCTCAC
    AAAGCTGAAA AGAAGCTTCA AAACATTGAC CACCCACTTA CAAAGTCATC CAGTAAAAGA
    GATCACCGGC GTTCTGTGGA TTCCAGAAAT ACCCTCAATG ATCTCCTGAA GCATTTAAAT
    GACCCAAACA GCAACCCCAA AGCCATCATG GGAGATATCC AGATGGCCCA CCAGACCCTA
    ATGCTGGATC CTGTGGGACC TATGTCTGAG GTCCCGCCCA AGGTCCCTAA CCGGGAGGCA
    TCCCTATACT CTCCTCCTTC AACTCTCCCC AGAAATAGCC CAACCAAGCG AGTGGATGTC
    CCCACCACTC CTGGGGTCCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA CCACAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCTGCTATG CCTAAAAACT TAAACTCACC AAATGGTGTT
    TTGTTATCCA GACAGCCTAG CATGAACCGT GGTGGGTATG TGCCCACCTC CACTGGGGCA
    AAGGTGGACT ATATTCAGGG AGCACCAGTG AGTGTTCATC TGCAGCCTTC CCTCTCCAGA
    CAGAGCAGCT ATACCAGTAA TGGCACCCTT CCTAGGACGG GACTAAAGAG GACACCGTCC
    TTAAAACCTG ATGTGCCACC AAAGCCTTCA TTTGTTCCTC AAACCACATC TGTCAGACCA
    CTGAACAAAT ACACTTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005638439.1
    CDS113..3373
    Translation

    Target ORF information:

    RefSeq Version XM_005638382.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (SEMA6D), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005638382.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGGTTCT TCCTGCTTTG TGCCTACATG CTGCTCCTGA TGATTTCCCA GCTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTC AATACTGTTG ATTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AATGAATCCC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAGTCCCCAA GACAGAAGTA ATACCAAGCA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ACCGAGAAAA CTGTGCTATG AAGGGCAAAC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTCCC AAGAAATGAT GAGATGGTTT TTGTATGTGG TACCAATGCA
    TTCAATCCCA TGTGCAGATA CTATAGGTTG AATACCTTAG AGTATGATGG AGAGGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTAT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC CCTTCGTACA ATAAAATATG ATTCCAAATG GATAAAAGAG
    CCACATTTTC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAATAATTT AGGCAAGGCT GTATATTCCC GTGTGGCCCG CATATGTAAA
    AATGACATGG GTGGCTCGCA GCGGGTCCTG GAGAAACACT GGACTTCATT TCTGAAGGCT
    CGGCTTAACT GCTCTGTCCC CGGAGATTCG TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACGGACATCA TACAAATCAA TGGCATCCCC ACTGTGGTCG GGGTGTTTAC CACACAGCTC
    AACAGCATTC CTGGGTCTGC AGTGTGTGCA TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGCT TTAAAGAACA GAAAACCCCA GATTCTGTTT GGACAGCAGT CCCTGAAGAC
    AAAGTACCCA AGCCAAGGCC TGGCTGTTGT GCAAAGCATG GCCTTGCCGA AGCTTATAAA
    ACCTCCATAG ATTTCCCTGA CGAAACCCTG TCATTCATCA AATCCCATCC GCTAATGGAC
    GCTGCTGTAC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCTGTTGA CCACACTGCT GGGCCCCATC AGAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTGCTTAAA GTTTTGGCAA AGACCAGCCC TTTCTCTTTG
    AATGACAGTG TATTACTGGA AGAGATTGAA GCATACAACC AAGCAAAGTG CAATGCTGAG
    AATGAGGAAG ACAGAAGGGT CATCTCCTTA CAGTTGGATA AAGACCATCA CGCTGTGTAC
    GTGGCATTCT CTAGCTGCGT CATCCGCCTC CCTCTCAGCC GTTGCGAGCG TTACGGATCC
    TGTAAGAAGT CTTGTGTTGC ATCTCGGGAC CCATACTGTG GCTGGTTAAG CCAGGGGGCC
    TGTGGCAGAG TGACCCCAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATATGA ACAAGACACA GAGTACGGCA ACACGGCCCA CCTAGGGGAC
    TGCCATGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCTTC ATCATCTGTT ACCACAATGG CAAGTATCCC AGAAATTACA
    CCTAAAGTGA TTGATACCTG GAGGCCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ACACTTCTGA TTTTACTGAT CCTTTATCGG GTATCCCAAA GGGTGTGCGA
    TGGGAAGTCC AATCGGGAGA GTCCAACCAG ATGGTCCACA TGAACGTCCT CATCACCTGT
    GTCTTTGCCG CTTTTGTTTT GGGTGCGTTC ATTGCAGGGG TGGCAGTATA CTGCTATCGT
    GACATGTTTG TTCGGAAAAA CAGAAAGATT CATAAGGATG CAGAATCTGC CCAGTCGTGC
    ACAGACTCCA GTGGAAGTTT TGCCAAACTG AATGGTCTGT TTGATAGCCC GGTCAAGGAA
    TATCAACAGA ATATCGATTC TCCCAAATTA TACAGTAACC TGCTGACTAG TCGGAAAGAG
    CTGCCACCCA ATGGAGATAC CAAATCCATG GTGATGGACC ATCGAGGCCA ACCTCCCGAA
    CTGGCTGCTC TCCCCACGCC TGAGTCAACT CCTGTGCTTC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCAGATAA GGCCCATGGC CATGGGGCTT CAAGGAAAGA AACCTCACAG
    TTTTTCCCAT CTAGTCCACC ACCCCATTCC CCATTAAGTC ATGGGCATAT CCCCAGTGCC
    ATTGTTCTTC CTAATGCTAC TCATGACTAC AACACGTCTT TCTCAAACTC CAATGCTCAC
    AAAGCTGAAA AGAAGCTTCA AAACATTGAC CACCCACTTA CAAAGTCATC CAGTAAAAGA
    GATCACCGGC GTTCTGTGGA TTCCAGAAAT ACCCTCAATG ATCTCCTGAA GCATTTAAAT
    GACCCAAACA GCAACCCCAA AGCCATCATG GGAGATATCC AGATGGCCCA CCAGACCCTA
    ATGCTGGATC CTGTGGGACC TATGTCTGAG GTCCCGCCCA AGGTCCCTAA CCGGGAGGCA
    TCCCTATACT CTCCTCCTTC AACTCTCCCC AGAAATAGCC CAACCAAGCG AGTGGATGTC
    CCCACCACTC CTGGGGTCCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA CCACAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCTGCTATG CCTAAAAACT TAAACTCACC AAATGGTGTT
    TTGTTATCCA GACAGCCTAG CATGAACCGT GGTGGGTATG TGCCCACCTC CACTGGGGCA
    AAGGTGGACT ATATTCAGGG AGCACCAGTG AGTGTTCATC TGCAGCCTTC CCTCTCCAGA
    CAGAGCAGCT ATACCAGTAA TGGCACCCTT CCTAGGACGG GACTAAAGAG GACACCGTCC
    TTAAAACCTG ATGTGCCACC AAAGCCTTCA TTTGTTCCTC AAACCACATC TGTCAGACCA
    CTGAACAAAT ACACTTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe28813
    Clone ID Related Accession (Same CDS sequence) XM_005638379.2 , XM_005638382.2 , XM_005638379.3 , XM_005638381.2 , XM_005638381.3 , XM_022413042.1
    Accession Version XM_005638379.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product semaphorin-6D isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005638379.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGGTTCT TCCTGCTTTG TGCCTACATG CTGCTCCTGA TGATTTCCCA GCTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTC AATACTGTTG ATTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AATGAATCCC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAGTCCCCAA GACAGAAGTA ATACCAAGCA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ACCGAGAAAA CTGTGCTATG AAGGGCAAAC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTCCC AAGAAATGAT GAGATGGTTT TTGTATGTGG TACCAATGCA
    TTCAATCCCA TGTGCAGATA CTATAGGTTG AATACCTTAG AGTATGATGG AGAGGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTAT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC CCTTCGTACA ATAAAATATG ATTCCAAATG GATAAAAGAG
    CCACATTTTC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAATAATTT AGGCAAGGCT GTATATTCCC GTGTGGCCCG CATATGTAAA
    AATGACATGG GTGGCTCGCA GCGGGTCCTG GAGAAACACT GGACTTCATT TCTGAAGGCT
    CGGCTTAACT GCTCTGTCCC CGGAGATTCG TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACGGACATCA TACAAATCAA TGGCATCCCC ACTGTGGTCG GGGTGTTTAC CACACAGCTC
    AACAGCATTC CTGGGTCTGC AGTGTGTGCA TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGCT TTAAAGAACA GAAAACCCCA GATTCTGTTT GGACAGCAGT CCCTGAAGAC
    AAAGTACCCA AGCCAAGGCC TGGCTGTTGT GCAAAGCATG GCCTTGCCGA AGCTTATAAA
    ACCTCCATAG ATTTCCCTGA CGAAACCCTG TCATTCATCA AATCCCATCC GCTAATGGAC
    GCTGCTGTAC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCTGTTGA CCACACTGCT GGGCCCCATC AGAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTGCTTAAA GTTTTGGCAA AGACCAGCCC TTTCTCTTTG
    AATGACAGTG TATTACTGGA AGAGATTGAA GCATACAACC AAGCAAAGTG CAATGCTGAG
    AATGAGGAAG ACAGAAGGGT CATCTCCTTA CAGTTGGATA AAGACCATCA CGCTGTGTAC
    GTGGCATTCT CTAGCTGCGT CATCCGCCTC CCTCTCAGCC GTTGCGAGCG TTACGGATCC
    TGTAAGAAGT CTTGTGTTGC ATCTCGGGAC CCATACTGTG GCTGGTTAAG CCAGGGGGCC
    TGTGGCAGAG TGACCCCAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATATGA ACAAGACACA GAGTACGGCA ACACGGCCCA CCTAGGGGAC
    TGCCATGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCTTC ATCATCTGTT ACCACAATGG CAAGTATCCC AGAAATTACA
    CCTAAAGTGA TTGATACCTG GAGGCCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ACACTTCTGA TTTTACTGAT CCTTTATCGG GTATCCCAAA GGGTGTGCGA
    TGGGAAGTCC AATCGGGAGA GTCCAACCAG ATGGTCCACA TGAACGTCCT CATCACCTGT
    GTCTTTGCCG CTTTTGTTTT GGGTGCGTTC ATTGCAGGGG TGGCAGTATA CTGCTATCGT
    GACATGTTTG TTCGGAAAAA CAGAAAGATT CATAAGGATG CAGAATCTGC CCAGTCGTGC
    ACAGACTCCA GTGGAAGTTT TGCCAAACTG AATGGTCTGT TTGATAGCCC GGTCAAGGAA
    TATCAACAGA ATATCGATTC TCCCAAATTA TACAGTAACC TGCTGACTAG TCGGAAAGAG
    CTGCCACCCA ATGGAGATAC CAAATCCATG GTGATGGACC ATCGAGGCCA ACCTCCCGAA
    CTGGCTGCTC TCCCCACGCC TGAGTCAACT CCTGTGCTTC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCAGATAA GGCCCATGGC CATGGGGCTT CAAGGAAAGA AACCTCACAG
    TTTTTCCCAT CTAGTCCACC ACCCCATTCC CCATTAAGTC ATGGGCATAT CCCCAGTGCC
    ATTGTTCTTC CTAATGCTAC TCATGACTAC AACACGTCTT TCTCAAACTC CAATGCTCAC
    AAAGCTGAAA AGAAGCTTCA AAACATTGAC CACCCACTTA CAAAGTCATC CAGTAAAAGA
    GATCACCGGC GTTCTGTGGA TTCCAGAAAT ACCCTCAATG ATCTCCTGAA GCATTTAAAT
    GACCCAAACA GCAACCCCAA AGCCATCATG GGAGATATCC AGATGGCCCA CCAGACCCTA
    ATGCTGGATC CTGTGGGACC TATGTCTGAG GTCCCGCCCA AGGTCCCTAA CCGGGAGGCA
    TCCCTATACT CTCCTCCTTC AACTCTCCCC AGAAATAGCC CAACCAAGCG AGTGGATGTC
    CCCACCACTC CTGGGGTCCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA CCACAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCTGCTATG CCTAAAAACT TAAACTCACC AAATGGTGTT
    TTGTTATCCA GACAGCCTAG CATGAACCGT GGTGGGTATG TGCCCACCTC CACTGGGGCA
    AAGGTGGACT ATATTCAGGG AGCACCAGTG AGTGTTCATC TGCAGCCTTC CCTCTCCAGA
    CAGAGCAGCT ATACCAGTAA TGGCACCCTT CCTAGGACGG GACTAAAGAG GACACCGTCC
    TTAAAACCTG ATGTGCCACC AAAGCCTTCA TTTGTTCCTC AAACCACATC TGTCAGACCA
    CTGAACAAAT ACACTTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005638436.1
    CDS287..3547
    Translation

    Target ORF information:

    RefSeq Version XM_005638379.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris semaphorin 6D (SEMA6D), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005638379.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGGTTCT TCCTGCTTTG TGCCTACATG CTGCTCCTGA TGATTTCCCA GCTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTC AATACTGTTG ATTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AATGAATCCC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAGTCCCCAA GACAGAAGTA ATACCAAGCA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ACCGAGAAAA CTGTGCTATG AAGGGCAAAC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTCCC AAGAAATGAT GAGATGGTTT TTGTATGTGG TACCAATGCA
    TTCAATCCCA TGTGCAGATA CTATAGGTTG AATACCTTAG AGTATGATGG AGAGGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTAT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC CCTTCGTACA ATAAAATATG ATTCCAAATG GATAAAAGAG
    CCACATTTTC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAATAATTT AGGCAAGGCT GTATATTCCC GTGTGGCCCG CATATGTAAA
    AATGACATGG GTGGCTCGCA GCGGGTCCTG GAGAAACACT GGACTTCATT TCTGAAGGCT
    CGGCTTAACT GCTCTGTCCC CGGAGATTCG TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACGGACATCA TACAAATCAA TGGCATCCCC ACTGTGGTCG GGGTGTTTAC CACACAGCTC
    AACAGCATTC CTGGGTCTGC AGTGTGTGCA TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGCT TTAAAGAACA GAAAACCCCA GATTCTGTTT GGACAGCAGT CCCTGAAGAC
    AAAGTACCCA AGCCAAGGCC TGGCTGTTGT GCAAAGCATG GCCTTGCCGA AGCTTATAAA
    ACCTCCATAG ATTTCCCTGA CGAAACCCTG TCATTCATCA AATCCCATCC GCTAATGGAC
    GCTGCTGTAC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCTGTTGA CCACACTGCT GGGCCCCATC AGAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTGCTTAAA GTTTTGGCAA AGACCAGCCC TTTCTCTTTG
    AATGACAGTG TATTACTGGA AGAGATTGAA GCATACAACC AAGCAAAGTG CAATGCTGAG
    AATGAGGAAG ACAGAAGGGT CATCTCCTTA CAGTTGGATA AAGACCATCA CGCTGTGTAC
    GTGGCATTCT CTAGCTGCGT CATCCGCCTC CCTCTCAGCC GTTGCGAGCG TTACGGATCC
    TGTAAGAAGT CTTGTGTTGC ATCTCGGGAC CCATACTGTG GCTGGTTAAG CCAGGGGGCC
    TGTGGCAGAG TGACCCCAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATATGA ACAAGACACA GAGTACGGCA ACACGGCCCA CCTAGGGGAC
    TGCCATGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCTTC ATCATCTGTT ACCACAATGG CAAGTATCCC AGAAATTACA
    CCTAAAGTGA TTGATACCTG GAGGCCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ACACTTCTGA TTTTACTGAT CCTTTATCGG GTATCCCAAA GGGTGTGCGA
    TGGGAAGTCC AATCGGGAGA GTCCAACCAG ATGGTCCACA TGAACGTCCT CATCACCTGT
    GTCTTTGCCG CTTTTGTTTT GGGTGCGTTC ATTGCAGGGG TGGCAGTATA CTGCTATCGT
    GACATGTTTG TTCGGAAAAA CAGAAAGATT CATAAGGATG CAGAATCTGC CCAGTCGTGC
    ACAGACTCCA GTGGAAGTTT TGCCAAACTG AATGGTCTGT TTGATAGCCC GGTCAAGGAA
    TATCAACAGA ATATCGATTC TCCCAAATTA TACAGTAACC TGCTGACTAG TCGGAAAGAG
    CTGCCACCCA ATGGAGATAC CAAATCCATG GTGATGGACC ATCGAGGCCA ACCTCCCGAA
    CTGGCTGCTC TCCCCACGCC TGAGTCAACT CCTGTGCTTC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCAGATAA GGCCCATGGC CATGGGGCTT CAAGGAAAGA AACCTCACAG
    TTTTTCCCAT CTAGTCCACC ACCCCATTCC CCATTAAGTC ATGGGCATAT CCCCAGTGCC
    ATTGTTCTTC CTAATGCTAC TCATGACTAC AACACGTCTT TCTCAAACTC CAATGCTCAC
    AAAGCTGAAA AGAAGCTTCA AAACATTGAC CACCCACTTA CAAAGTCATC CAGTAAAAGA
    GATCACCGGC GTTCTGTGGA TTCCAGAAAT ACCCTCAATG ATCTCCTGAA GCATTTAAAT
    GACCCAAACA GCAACCCCAA AGCCATCATG GGAGATATCC AGATGGCCCA CCAGACCCTA
    ATGCTGGATC CTGTGGGACC TATGTCTGAG GTCCCGCCCA AGGTCCCTAA CCGGGAGGCA
    TCCCTATACT CTCCTCCTTC AACTCTCCCC AGAAATAGCC CAACCAAGCG AGTGGATGTC
    CCCACCACTC CTGGGGTCCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA CCACAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCTGCTATG CCTAAAAACT TAAACTCACC AAATGGTGTT
    TTGTTATCCA GACAGCCTAG CATGAACCGT GGTGGGTATG TGCCCACCTC CACTGGGGCA
    AAGGTGGACT ATATTCAGGG AGCACCAGTG AGTGTTCATC TGCAGCCTTC CCTCTCCAGA
    CAGAGCAGCT ATACCAGTAA TGGCACCCTT CCTAGGACGG GACTAAAGAG GACACCGTCC
    TTAAAACCTG ATGTGCCACC AAAGCCTTCA TTTGTTCCTC AAACCACATC TGTCAGACCA
    CTGAACAAAT ACACTTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe28813
    Clone ID Related Accession (Same CDS sequence) XM_005638379.2 , XM_005638382.2 , XM_005638379.3 , XM_005638381.2 , XM_005638381.3 , XM_022413042.1
    Accession Version XM_005638381.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product semaphorin-6D isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005638381.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGGTTCT TCCTGCTTTG TGCCTACATG CTGCTCCTGA TGATTTCCCA GCTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTC AATACTGTTG ATTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AATGAATCCC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAGTCCCCAA GACAGAAGTA ATACCAAGCA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ACCGAGAAAA CTGTGCTATG AAGGGCAAAC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTCCC AAGAAATGAT GAGATGGTTT TTGTATGTGG TACCAATGCA
    TTCAATCCCA TGTGCAGATA CTATAGGTTG AATACCTTAG AGTATGATGG AGAGGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTAT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC CCTTCGTACA ATAAAATATG ATTCCAAATG GATAAAAGAG
    CCACATTTTC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAATAATTT AGGCAAGGCT GTATATTCCC GTGTGGCCCG CATATGTAAA
    AATGACATGG GTGGCTCGCA GCGGGTCCTG GAGAAACACT GGACTTCATT TCTGAAGGCT
    CGGCTTAACT GCTCTGTCCC CGGAGATTCG TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACGGACATCA TACAAATCAA TGGCATCCCC ACTGTGGTCG GGGTGTTTAC CACACAGCTC
    AACAGCATTC CTGGGTCTGC AGTGTGTGCA TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGCT TTAAAGAACA GAAAACCCCA GATTCTGTTT GGACAGCAGT CCCTGAAGAC
    AAAGTACCCA AGCCAAGGCC TGGCTGTTGT GCAAAGCATG GCCTTGCCGA AGCTTATAAA
    ACCTCCATAG ATTTCCCTGA CGAAACCCTG TCATTCATCA AATCCCATCC GCTAATGGAC
    GCTGCTGTAC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCTGTTGA CCACACTGCT GGGCCCCATC AGAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTGCTTAAA GTTTTGGCAA AGACCAGCCC TTTCTCTTTG
    AATGACAGTG TATTACTGGA AGAGATTGAA GCATACAACC AAGCAAAGTG CAATGCTGAG
    AATGAGGAAG ACAGAAGGGT CATCTCCTTA CAGTTGGATA AAGACCATCA CGCTGTGTAC
    GTGGCATTCT CTAGCTGCGT CATCCGCCTC CCTCTCAGCC GTTGCGAGCG TTACGGATCC
    TGTAAGAAGT CTTGTGTTGC ATCTCGGGAC CCATACTGTG GCTGGTTAAG CCAGGGGGCC
    TGTGGCAGAG TGACCCCAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATATGA ACAAGACACA GAGTACGGCA ACACGGCCCA CCTAGGGGAC
    TGCCATGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCTTC ATCATCTGTT ACCACAATGG CAAGTATCCC AGAAATTACA
    CCTAAAGTGA TTGATACCTG GAGGCCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ACACTTCTGA TTTTACTGAT CCTTTATCGG GTATCCCAAA GGGTGTGCGA
    TGGGAAGTCC AATCGGGAGA GTCCAACCAG ATGGTCCACA TGAACGTCCT CATCACCTGT
    GTCTTTGCCG CTTTTGTTTT GGGTGCGTTC ATTGCAGGGG TGGCAGTATA CTGCTATCGT
    GACATGTTTG TTCGGAAAAA CAGAAAGATT CATAAGGATG CAGAATCTGC CCAGTCGTGC
    ACAGACTCCA GTGGAAGTTT TGCCAAACTG AATGGTCTGT TTGATAGCCC GGTCAAGGAA
    TATCAACAGA ATATCGATTC TCCCAAATTA TACAGTAACC TGCTGACTAG TCGGAAAGAG
    CTGCCACCCA ATGGAGATAC CAAATCCATG GTGATGGACC ATCGAGGCCA ACCTCCCGAA
    CTGGCTGCTC TCCCCACGCC TGAGTCAACT CCTGTGCTTC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCAGATAA GGCCCATGGC CATGGGGCTT CAAGGAAAGA AACCTCACAG
    TTTTTCCCAT CTAGTCCACC ACCCCATTCC CCATTAAGTC ATGGGCATAT CCCCAGTGCC
    ATTGTTCTTC CTAATGCTAC TCATGACTAC AACACGTCTT TCTCAAACTC CAATGCTCAC
    AAAGCTGAAA AGAAGCTTCA AAACATTGAC CACCCACTTA CAAAGTCATC CAGTAAAAGA
    GATCACCGGC GTTCTGTGGA TTCCAGAAAT ACCCTCAATG ATCTCCTGAA GCATTTAAAT
    GACCCAAACA GCAACCCCAA AGCCATCATG GGAGATATCC AGATGGCCCA CCAGACCCTA
    ATGCTGGATC CTGTGGGACC TATGTCTGAG GTCCCGCCCA AGGTCCCTAA CCGGGAGGCA
    TCCCTATACT CTCCTCCTTC AACTCTCCCC AGAAATAGCC CAACCAAGCG AGTGGATGTC
    CCCACCACTC CTGGGGTCCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA CCACAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCTGCTATG CCTAAAAACT TAAACTCACC AAATGGTGTT
    TTGTTATCCA GACAGCCTAG CATGAACCGT GGTGGGTATG TGCCCACCTC CACTGGGGCA
    AAGGTGGACT ATATTCAGGG AGCACCAGTG AGTGTTCATC TGCAGCCTTC CCTCTCCAGA
    CAGAGCAGCT ATACCAGTAA TGGCACCCTT CCTAGGACGG GACTAAAGAG GACACCGTCC
    TTAAAACCTG ATGTGCCACC AAAGCCTTCA TTTGTTCCTC AAACCACATC TGTCAGACCA
    CTGAACAAAT ACACTTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005638438.1
    CDS113..3373
    Translation

    Target ORF information:

    RefSeq Version XM_005638381.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (SEMA6D), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005638381.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGGTTCT TCCTGCTTTG TGCCTACATG CTGCTCCTGA TGATTTCCCA GCTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTC AATACTGTTG ATTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AATGAATCCC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAGTCCCCAA GACAGAAGTA ATACCAAGCA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ACCGAGAAAA CTGTGCTATG AAGGGCAAAC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTCCC AAGAAATGAT GAGATGGTTT TTGTATGTGG TACCAATGCA
    TTCAATCCCA TGTGCAGATA CTATAGGTTG AATACCTTAG AGTATGATGG AGAGGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTAT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC CCTTCGTACA ATAAAATATG ATTCCAAATG GATAAAAGAG
    CCACATTTTC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAATAATTT AGGCAAGGCT GTATATTCCC GTGTGGCCCG CATATGTAAA
    AATGACATGG GTGGCTCGCA GCGGGTCCTG GAGAAACACT GGACTTCATT TCTGAAGGCT
    CGGCTTAACT GCTCTGTCCC CGGAGATTCG TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACGGACATCA TACAAATCAA TGGCATCCCC ACTGTGGTCG GGGTGTTTAC CACACAGCTC
    AACAGCATTC CTGGGTCTGC AGTGTGTGCA TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGCT TTAAAGAACA GAAAACCCCA GATTCTGTTT GGACAGCAGT CCCTGAAGAC
    AAAGTACCCA AGCCAAGGCC TGGCTGTTGT GCAAAGCATG GCCTTGCCGA AGCTTATAAA
    ACCTCCATAG ATTTCCCTGA CGAAACCCTG TCATTCATCA AATCCCATCC GCTAATGGAC
    GCTGCTGTAC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCTGTTGA CCACACTGCT GGGCCCCATC AGAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTGCTTAAA GTTTTGGCAA AGACCAGCCC TTTCTCTTTG
    AATGACAGTG TATTACTGGA AGAGATTGAA GCATACAACC AAGCAAAGTG CAATGCTGAG
    AATGAGGAAG ACAGAAGGGT CATCTCCTTA CAGTTGGATA AAGACCATCA CGCTGTGTAC
    GTGGCATTCT CTAGCTGCGT CATCCGCCTC CCTCTCAGCC GTTGCGAGCG TTACGGATCC
    TGTAAGAAGT CTTGTGTTGC ATCTCGGGAC CCATACTGTG GCTGGTTAAG CCAGGGGGCC
    TGTGGCAGAG TGACCCCAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATATGA ACAAGACACA GAGTACGGCA ACACGGCCCA CCTAGGGGAC
    TGCCATGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCTTC ATCATCTGTT ACCACAATGG CAAGTATCCC AGAAATTACA
    CCTAAAGTGA TTGATACCTG GAGGCCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ACACTTCTGA TTTTACTGAT CCTTTATCGG GTATCCCAAA GGGTGTGCGA
    TGGGAAGTCC AATCGGGAGA GTCCAACCAG ATGGTCCACA TGAACGTCCT CATCACCTGT
    GTCTTTGCCG CTTTTGTTTT GGGTGCGTTC ATTGCAGGGG TGGCAGTATA CTGCTATCGT
    GACATGTTTG TTCGGAAAAA CAGAAAGATT CATAAGGATG CAGAATCTGC CCAGTCGTGC
    ACAGACTCCA GTGGAAGTTT TGCCAAACTG AATGGTCTGT TTGATAGCCC GGTCAAGGAA
    TATCAACAGA ATATCGATTC TCCCAAATTA TACAGTAACC TGCTGACTAG TCGGAAAGAG
    CTGCCACCCA ATGGAGATAC CAAATCCATG GTGATGGACC ATCGAGGCCA ACCTCCCGAA
    CTGGCTGCTC TCCCCACGCC TGAGTCAACT CCTGTGCTTC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCAGATAA GGCCCATGGC CATGGGGCTT CAAGGAAAGA AACCTCACAG
    TTTTTCCCAT CTAGTCCACC ACCCCATTCC CCATTAAGTC ATGGGCATAT CCCCAGTGCC
    ATTGTTCTTC CTAATGCTAC TCATGACTAC AACACGTCTT TCTCAAACTC CAATGCTCAC
    AAAGCTGAAA AGAAGCTTCA AAACATTGAC CACCCACTTA CAAAGTCATC CAGTAAAAGA
    GATCACCGGC GTTCTGTGGA TTCCAGAAAT ACCCTCAATG ATCTCCTGAA GCATTTAAAT
    GACCCAAACA GCAACCCCAA AGCCATCATG GGAGATATCC AGATGGCCCA CCAGACCCTA
    ATGCTGGATC CTGTGGGACC TATGTCTGAG GTCCCGCCCA AGGTCCCTAA CCGGGAGGCA
    TCCCTATACT CTCCTCCTTC AACTCTCCCC AGAAATAGCC CAACCAAGCG AGTGGATGTC
    CCCACCACTC CTGGGGTCCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA CCACAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCTGCTATG CCTAAAAACT TAAACTCACC AAATGGTGTT
    TTGTTATCCA GACAGCCTAG CATGAACCGT GGTGGGTATG TGCCCACCTC CACTGGGGCA
    AAGGTGGACT ATATTCAGGG AGCACCAGTG AGTGTTCATC TGCAGCCTTC CCTCTCCAGA
    CAGAGCAGCT ATACCAGTAA TGGCACCCTT CCTAGGACGG GACTAAAGAG GACACCGTCC
    TTAAAACCTG ATGTGCCACC AAAGCCTTCA TTTGTTCCTC AAACCACATC TGTCAGACCA
    CTGAACAAAT ACACTTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe28813
    Clone ID Related Accession (Same CDS sequence) XM_005638379.2 , XM_005638382.2 , XM_005638379.3 , XM_005638381.2 , XM_005638381.3 , XM_022413042.1
    Accession Version XM_005638381.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product semaphorin-6D isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005638381.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGGTTCT TCCTGCTTTG TGCCTACATG CTGCTCCTGA TGATTTCCCA GCTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTC AATACTGTTG ATTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AATGAATCCC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAGTCCCCAA GACAGAAGTA ATACCAAGCA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ACCGAGAAAA CTGTGCTATG AAGGGCAAAC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTCCC AAGAAATGAT GAGATGGTTT TTGTATGTGG TACCAATGCA
    TTCAATCCCA TGTGCAGATA CTATAGGTTG AATACCTTAG AGTATGATGG AGAGGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTAT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC CCTTCGTACA ATAAAATATG ATTCCAAATG GATAAAAGAG
    CCACATTTTC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAATAATTT AGGCAAGGCT GTATATTCCC GTGTGGCCCG CATATGTAAA
    AATGACATGG GTGGCTCGCA GCGGGTCCTG GAGAAACACT GGACTTCATT TCTGAAGGCT
    CGGCTTAACT GCTCTGTCCC CGGAGATTCG TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACGGACATCA TACAAATCAA TGGCATCCCC ACTGTGGTCG GGGTGTTTAC CACACAGCTC
    AACAGCATTC CTGGGTCTGC AGTGTGTGCA TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGCT TTAAAGAACA GAAAACCCCA GATTCTGTTT GGACAGCAGT CCCTGAAGAC
    AAAGTACCCA AGCCAAGGCC TGGCTGTTGT GCAAAGCATG GCCTTGCCGA AGCTTATAAA
    ACCTCCATAG ATTTCCCTGA CGAAACCCTG TCATTCATCA AATCCCATCC GCTAATGGAC
    GCTGCTGTAC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCTGTTGA CCACACTGCT GGGCCCCATC AGAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTGCTTAAA GTTTTGGCAA AGACCAGCCC TTTCTCTTTG
    AATGACAGTG TATTACTGGA AGAGATTGAA GCATACAACC AAGCAAAGTG CAATGCTGAG
    AATGAGGAAG ACAGAAGGGT CATCTCCTTA CAGTTGGATA AAGACCATCA CGCTGTGTAC
    GTGGCATTCT CTAGCTGCGT CATCCGCCTC CCTCTCAGCC GTTGCGAGCG TTACGGATCC
    TGTAAGAAGT CTTGTGTTGC ATCTCGGGAC CCATACTGTG GCTGGTTAAG CCAGGGGGCC
    TGTGGCAGAG TGACCCCAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATATGA ACAAGACACA GAGTACGGCA ACACGGCCCA CCTAGGGGAC
    TGCCATGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCTTC ATCATCTGTT ACCACAATGG CAAGTATCCC AGAAATTACA
    CCTAAAGTGA TTGATACCTG GAGGCCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ACACTTCTGA TTTTACTGAT CCTTTATCGG GTATCCCAAA GGGTGTGCGA
    TGGGAAGTCC AATCGGGAGA GTCCAACCAG ATGGTCCACA TGAACGTCCT CATCACCTGT
    GTCTTTGCCG CTTTTGTTTT GGGTGCGTTC ATTGCAGGGG TGGCAGTATA CTGCTATCGT
    GACATGTTTG TTCGGAAAAA CAGAAAGATT CATAAGGATG CAGAATCTGC CCAGTCGTGC
    ACAGACTCCA GTGGAAGTTT TGCCAAACTG AATGGTCTGT TTGATAGCCC GGTCAAGGAA
    TATCAACAGA ATATCGATTC TCCCAAATTA TACAGTAACC TGCTGACTAG TCGGAAAGAG
    CTGCCACCCA ATGGAGATAC CAAATCCATG GTGATGGACC ATCGAGGCCA ACCTCCCGAA
    CTGGCTGCTC TCCCCACGCC TGAGTCAACT CCTGTGCTTC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCAGATAA GGCCCATGGC CATGGGGCTT CAAGGAAAGA AACCTCACAG
    TTTTTCCCAT CTAGTCCACC ACCCCATTCC CCATTAAGTC ATGGGCATAT CCCCAGTGCC
    ATTGTTCTTC CTAATGCTAC TCATGACTAC AACACGTCTT TCTCAAACTC CAATGCTCAC
    AAAGCTGAAA AGAAGCTTCA AAACATTGAC CACCCACTTA CAAAGTCATC CAGTAAAAGA
    GATCACCGGC GTTCTGTGGA TTCCAGAAAT ACCCTCAATG ATCTCCTGAA GCATTTAAAT
    GACCCAAACA GCAACCCCAA AGCCATCATG GGAGATATCC AGATGGCCCA CCAGACCCTA
    ATGCTGGATC CTGTGGGACC TATGTCTGAG GTCCCGCCCA AGGTCCCTAA CCGGGAGGCA
    TCCCTATACT CTCCTCCTTC AACTCTCCCC AGAAATAGCC CAACCAAGCG AGTGGATGTC
    CCCACCACTC CTGGGGTCCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA CCACAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCTGCTATG CCTAAAAACT TAAACTCACC AAATGGTGTT
    TTGTTATCCA GACAGCCTAG CATGAACCGT GGTGGGTATG TGCCCACCTC CACTGGGGCA
    AAGGTGGACT ATATTCAGGG AGCACCAGTG AGTGTTCATC TGCAGCCTTC CCTCTCCAGA
    CAGAGCAGCT ATACCAGTAA TGGCACCCTT CCTAGGACGG GACTAAAGAG GACACCGTCC
    TTAAAACCTG ATGTGCCACC AAAGCCTTCA TTTGTTCCTC AAACCACATC TGTCAGACCA
    CTGAACAAAT ACACTTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005638438.1
    CDS154..3414
    Translation

    Target ORF information:

    RefSeq Version XM_005638381.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris semaphorin 6D (SEMA6D), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005638381.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGGTTCT TCCTGCTTTG TGCCTACATG CTGCTCCTGA TGATTTCCCA GCTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTC AATACTGTTG ATTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AATGAATCCC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAGTCCCCAA GACAGAAGTA ATACCAAGCA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ACCGAGAAAA CTGTGCTATG AAGGGCAAAC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTCCC AAGAAATGAT GAGATGGTTT TTGTATGTGG TACCAATGCA
    TTCAATCCCA TGTGCAGATA CTATAGGTTG AATACCTTAG AGTATGATGG AGAGGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTAT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC CCTTCGTACA ATAAAATATG ATTCCAAATG GATAAAAGAG
    CCACATTTTC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAATAATTT AGGCAAGGCT GTATATTCCC GTGTGGCCCG CATATGTAAA
    AATGACATGG GTGGCTCGCA GCGGGTCCTG GAGAAACACT GGACTTCATT TCTGAAGGCT
    CGGCTTAACT GCTCTGTCCC CGGAGATTCG TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACGGACATCA TACAAATCAA TGGCATCCCC ACTGTGGTCG GGGTGTTTAC CACACAGCTC
    AACAGCATTC CTGGGTCTGC AGTGTGTGCA TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGCT TTAAAGAACA GAAAACCCCA GATTCTGTTT GGACAGCAGT CCCTGAAGAC
    AAAGTACCCA AGCCAAGGCC TGGCTGTTGT GCAAAGCATG GCCTTGCCGA AGCTTATAAA
    ACCTCCATAG ATTTCCCTGA CGAAACCCTG TCATTCATCA AATCCCATCC GCTAATGGAC
    GCTGCTGTAC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCTGTTGA CCACACTGCT GGGCCCCATC AGAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTGCTTAAA GTTTTGGCAA AGACCAGCCC TTTCTCTTTG
    AATGACAGTG TATTACTGGA AGAGATTGAA GCATACAACC AAGCAAAGTG CAATGCTGAG
    AATGAGGAAG ACAGAAGGGT CATCTCCTTA CAGTTGGATA AAGACCATCA CGCTGTGTAC
    GTGGCATTCT CTAGCTGCGT CATCCGCCTC CCTCTCAGCC GTTGCGAGCG TTACGGATCC
    TGTAAGAAGT CTTGTGTTGC ATCTCGGGAC CCATACTGTG GCTGGTTAAG CCAGGGGGCC
    TGTGGCAGAG TGACCCCAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATATGA ACAAGACACA GAGTACGGCA ACACGGCCCA CCTAGGGGAC
    TGCCATGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCTTC ATCATCTGTT ACCACAATGG CAAGTATCCC AGAAATTACA
    CCTAAAGTGA TTGATACCTG GAGGCCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ACACTTCTGA TTTTACTGAT CCTTTATCGG GTATCCCAAA GGGTGTGCGA
    TGGGAAGTCC AATCGGGAGA GTCCAACCAG ATGGTCCACA TGAACGTCCT CATCACCTGT
    GTCTTTGCCG CTTTTGTTTT GGGTGCGTTC ATTGCAGGGG TGGCAGTATA CTGCTATCGT
    GACATGTTTG TTCGGAAAAA CAGAAAGATT CATAAGGATG CAGAATCTGC CCAGTCGTGC
    ACAGACTCCA GTGGAAGTTT TGCCAAACTG AATGGTCTGT TTGATAGCCC GGTCAAGGAA
    TATCAACAGA ATATCGATTC TCCCAAATTA TACAGTAACC TGCTGACTAG TCGGAAAGAG
    CTGCCACCCA ATGGAGATAC CAAATCCATG GTGATGGACC ATCGAGGCCA ACCTCCCGAA
    CTGGCTGCTC TCCCCACGCC TGAGTCAACT CCTGTGCTTC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCAGATAA GGCCCATGGC CATGGGGCTT CAAGGAAAGA AACCTCACAG
    TTTTTCCCAT CTAGTCCACC ACCCCATTCC CCATTAAGTC ATGGGCATAT CCCCAGTGCC
    ATTGTTCTTC CTAATGCTAC TCATGACTAC AACACGTCTT TCTCAAACTC CAATGCTCAC
    AAAGCTGAAA AGAAGCTTCA AAACATTGAC CACCCACTTA CAAAGTCATC CAGTAAAAGA
    GATCACCGGC GTTCTGTGGA TTCCAGAAAT ACCCTCAATG ATCTCCTGAA GCATTTAAAT
    GACCCAAACA GCAACCCCAA AGCCATCATG GGAGATATCC AGATGGCCCA CCAGACCCTA
    ATGCTGGATC CTGTGGGACC TATGTCTGAG GTCCCGCCCA AGGTCCCTAA CCGGGAGGCA
    TCCCTATACT CTCCTCCTTC AACTCTCCCC AGAAATAGCC CAACCAAGCG AGTGGATGTC
    CCCACCACTC CTGGGGTCCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA CCACAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCTGCTATG CCTAAAAACT TAAACTCACC AAATGGTGTT
    TTGTTATCCA GACAGCCTAG CATGAACCGT GGTGGGTATG TGCCCACCTC CACTGGGGCA
    AAGGTGGACT ATATTCAGGG AGCACCAGTG AGTGTTCATC TGCAGCCTTC CCTCTCCAGA
    CAGAGCAGCT ATACCAGTAA TGGCACCCTT CCTAGGACGG GACTAAAGAG GACACCGTCC
    TTAAAACCTG ATGTGCCACC AAAGCCTTCA TTTGTTCCTC AAACCACATC TGTCAGACCA
    CTGAACAAAT ACACTTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe28813
    Clone ID Related Accession (Same CDS sequence) XM_005638379.2 , XM_005638382.2 , XM_005638379.3 , XM_005638381.2 , XM_005638381.3 , XM_022413042.1
    Accession Version XM_022413042.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product semaphorin-6D isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGGTTCT TCCTGCTTTG TGCCTACATG CTGCTCCTGA TGATTTCCCA GCTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTC AATACTGTTG ATTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AATGAATCCC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAGTCCCCAA GACAGAAGTA ATACCAAGCA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ACCGAGAAAA CTGTGCTATG AAGGGCAAAC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTCCC AAGAAATGAT GAGATGGTTT TTGTATGTGG TACCAATGCA
    TTCAATCCCA TGTGCAGATA CTATAGGTTG AATACCTTAG AGTATGATGG AGAGGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTAT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC CCTTCGTACA ATAAAATATG ATTCCAAATG GATAAAAGAG
    CCACATTTTC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAATAATTT AGGCAAGGCT GTATATTCCC GTGTGGCCCG CATATGTAAA
    AATGACATGG GTGGCTCGCA GCGGGTCCTG GAGAAACACT GGACTTCATT TCTGAAGGCT
    CGGCTTAACT GCTCTGTCCC CGGAGATTCG TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACGGACATCA TACAAATCAA TGGCATCCCC ACTGTGGTCG GGGTGTTTAC CACACAGCTC
    AACAGCATTC CTGGGTCTGC AGTGTGTGCA TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGCT TTAAAGAACA GAAAACCCCA GATTCTGTTT GGACAGCAGT CCCTGAAGAC
    AAAGTACCCA AGCCAAGGCC TGGCTGTTGT GCAAAGCATG GCCTTGCCGA AGCTTATAAA
    ACCTCCATAG ATTTCCCTGA CGAAACCCTG TCATTCATCA AATCCCATCC GCTAATGGAC
    GCTGCTGTAC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCTGTTGA CCACACTGCT GGGCCCCATC AGAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTGCTTAAA GTTTTGGCAA AGACCAGCCC TTTCTCTTTG
    AATGACAGTG TATTACTGGA AGAGATTGAA GCATACAACC AAGCAAAGTG CAATGCTGAG
    AATGAGGAAG ACAGAAGGGT CATCTCCTTA CAGTTGGATA AAGACCATCA CGCTGTGTAC
    GTGGCATTCT CTAGCTGCGT CATCCGCCTC CCTCTCAGCC GTTGCGAGCG TTACGGATCC
    TGTAAGAAGT CTTGTGTTGC ATCTCGGGAC CCATACTGTG GCTGGTTAAG CCAGGGGGCC
    TGTGGCAGAG TGACCCCAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATATGA ACAAGACACA GAGTACGGCA ACACGGCCCA CCTAGGGGAC
    TGCCATGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCTTC ATCATCTGTT ACCACAATGG CAAGTATCCC AGAAATTACA
    CCTAAAGTGA TTGATACCTG GAGGCCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ACACTTCTGA TTTTACTGAT CCTTTATCGG GTATCCCAAA GGGTGTGCGA
    TGGGAAGTCC AATCGGGAGA GTCCAACCAG ATGGTCCACA TGAACGTCCT CATCACCTGT
    GTCTTTGCCG CTTTTGTTTT GGGTGCGTTC ATTGCAGGGG TGGCAGTATA CTGCTATCGT
    GACATGTTTG TTCGGAAAAA CAGAAAGATT CATAAGGATG CAGAATCTGC CCAGTCGTGC
    ACAGACTCCA GTGGAAGTTT TGCCAAACTG AATGGTCTGT TTGATAGCCC GGTCAAGGAA
    TATCAACAGA ATATCGATTC TCCCAAATTA TACAGTAACC TGCTGACTAG TCGGAAAGAG
    CTGCCACCCA ATGGAGATAC CAAATCCATG GTGATGGACC ATCGAGGCCA ACCTCCCGAA
    CTGGCTGCTC TCCCCACGCC TGAGTCAACT CCTGTGCTTC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCAGATAA GGCCCATGGC CATGGGGCTT CAAGGAAAGA AACCTCACAG
    TTTTTCCCAT CTAGTCCACC ACCCCATTCC CCATTAAGTC ATGGGCATAT CCCCAGTGCC
    ATTGTTCTTC CTAATGCTAC TCATGACTAC AACACGTCTT TCTCAAACTC CAATGCTCAC
    AAAGCTGAAA AGAAGCTTCA AAACATTGAC CACCCACTTA CAAAGTCATC CAGTAAAAGA
    GATCACCGGC GTTCTGTGGA TTCCAGAAAT ACCCTCAATG ATCTCCTGAA GCATTTAAAT
    GACCCAAACA GCAACCCCAA AGCCATCATG GGAGATATCC AGATGGCCCA CCAGACCCTA
    ATGCTGGATC CTGTGGGACC TATGTCTGAG GTCCCGCCCA AGGTCCCTAA CCGGGAGGCA
    TCCCTATACT CTCCTCCTTC AACTCTCCCC AGAAATAGCC CAACCAAGCG AGTGGATGTC
    CCCACCACTC CTGGGGTCCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA CCACAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCTGCTATG CCTAAAAACT TAAACTCACC AAATGGTGTT
    TTGTTATCCA GACAGCCTAG CATGAACCGT GGTGGGTATG TGCCCACCTC CACTGGGGCA
    AAGGTGGACT ATATTCAGGG AGCACCAGTG AGTGTTCATC TGCAGCCTTC CCTCTCCAGA
    CAGAGCAGCT ATACCAGTAA TGGCACCCTT CCTAGGACGG GACTAAAGAG GACACCGTCC
    TTAAAACCTG ATGTGCCACC AAAGCCTTCA TTTGTTCCTC AAACCACATC TGTCAGACCA
    CTGAACAAAT ACACTTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268750.1
    CDS336..3596
    Translation

    Target ORF information:

    RefSeq Version XM_022413042.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris semaphorin 6D (SEMA6D), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413042.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGGTTCT TCCTGCTTTG TGCCTACATG CTGCTCCTGA TGATTTCCCA GCTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTC AATACTGTTG ATTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AATGAATCCC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAGTCCCCAA GACAGAAGTA ATACCAAGCA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ACCGAGAAAA CTGTGCTATG AAGGGCAAAC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTCCC AAGAAATGAT GAGATGGTTT TTGTATGTGG TACCAATGCA
    TTCAATCCCA TGTGCAGATA CTATAGGTTG AATACCTTAG AGTATGATGG AGAGGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTAT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC CCTTCGTACA ATAAAATATG ATTCCAAATG GATAAAAGAG
    CCACATTTTC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAATAATTT AGGCAAGGCT GTATATTCCC GTGTGGCCCG CATATGTAAA
    AATGACATGG GTGGCTCGCA GCGGGTCCTG GAGAAACACT GGACTTCATT TCTGAAGGCT
    CGGCTTAACT GCTCTGTCCC CGGAGATTCG TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACGGACATCA TACAAATCAA TGGCATCCCC ACTGTGGTCG GGGTGTTTAC CACACAGCTC
    AACAGCATTC CTGGGTCTGC AGTGTGTGCA TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGCT TTAAAGAACA GAAAACCCCA GATTCTGTTT GGACAGCAGT CCCTGAAGAC
    AAAGTACCCA AGCCAAGGCC TGGCTGTTGT GCAAAGCATG GCCTTGCCGA AGCTTATAAA
    ACCTCCATAG ATTTCCCTGA CGAAACCCTG TCATTCATCA AATCCCATCC GCTAATGGAC
    GCTGCTGTAC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCTGTTGA CCACACTGCT GGGCCCCATC AGAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTGCTTAAA GTTTTGGCAA AGACCAGCCC TTTCTCTTTG
    AATGACAGTG TATTACTGGA AGAGATTGAA GCATACAACC AAGCAAAGTG CAATGCTGAG
    AATGAGGAAG ACAGAAGGGT CATCTCCTTA CAGTTGGATA AAGACCATCA CGCTGTGTAC
    GTGGCATTCT CTAGCTGCGT CATCCGCCTC CCTCTCAGCC GTTGCGAGCG TTACGGATCC
    TGTAAGAAGT CTTGTGTTGC ATCTCGGGAC CCATACTGTG GCTGGTTAAG CCAGGGGGCC
    TGTGGCAGAG TGACCCCAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATATGA ACAAGACACA GAGTACGGCA ACACGGCCCA CCTAGGGGAC
    TGCCATGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGACATGG AGGTATCTTC ATCATCTGTT ACCACAATGG CAAGTATCCC AGAAATTACA
    CCTAAAGTGA TTGATACCTG GAGGCCTAAA CTGACGAGCT CCCGGAAATT TGTAGTTCAA
    GATGACCCAA ACACTTCTGA TTTTACTGAT CCTTTATCGG GTATCCCAAA GGGTGTGCGA
    TGGGAAGTCC AATCGGGAGA GTCCAACCAG ATGGTCCACA TGAACGTCCT CATCACCTGT
    GTCTTTGCCG CTTTTGTTTT GGGTGCGTTC ATTGCAGGGG TGGCAGTATA CTGCTATCGT
    GACATGTTTG TTCGGAAAAA CAGAAAGATT CATAAGGATG CAGAATCTGC CCAGTCGTGC
    ACAGACTCCA GTGGAAGTTT TGCCAAACTG AATGGTCTGT TTGATAGCCC GGTCAAGGAA
    TATCAACAGA ATATCGATTC TCCCAAATTA TACAGTAACC TGCTGACTAG TCGGAAAGAG
    CTGCCACCCA ATGGAGATAC CAAATCCATG GTGATGGACC ATCGAGGCCA ACCTCCCGAA
    CTGGCTGCTC TCCCCACGCC TGAGTCAACT CCTGTGCTTC ACCAGAAGAC CCTGCAGGCC
    ATGAAGAGCC ACTCAGATAA GGCCCATGGC CATGGGGCTT CAAGGAAAGA AACCTCACAG
    TTTTTCCCAT CTAGTCCACC ACCCCATTCC CCATTAAGTC ATGGGCATAT CCCCAGTGCC
    ATTGTTCTTC CTAATGCTAC TCATGACTAC AACACGTCTT TCTCAAACTC CAATGCTCAC
    AAAGCTGAAA AGAAGCTTCA AAACATTGAC CACCCACTTA CAAAGTCATC CAGTAAAAGA
    GATCACCGGC GTTCTGTGGA TTCCAGAAAT ACCCTCAATG ATCTCCTGAA GCATTTAAAT
    GACCCAAACA GCAACCCCAA AGCCATCATG GGAGATATCC AGATGGCCCA CCAGACCCTA
    ATGCTGGATC CTGTGGGACC TATGTCTGAG GTCCCGCCCA AGGTCCCTAA CCGGGAGGCA
    TCCCTATACT CTCCTCCTTC AACTCTCCCC AGAAATAGCC CAACCAAGCG AGTGGATGTC
    CCCACCACTC CTGGGGTCCC AATGACTTCT CTGGAAAGAC AAAGGGGTTA CCACAAAAAT
    TCCTCCCAGA GGCACTCTAT ATCTGCTATG CCTAAAAACT TAAACTCACC AAATGGTGTT
    TTGTTATCCA GACAGCCTAG CATGAACCGT GGTGGGTATG TGCCCACCTC CACTGGGGCA
    AAGGTGGACT ATATTCAGGG AGCACCAGTG AGTGTTCATC TGCAGCCTTC CCTCTCCAGA
    CAGAGCAGCT ATACCAGTAA TGGCACCCTT CCTAGGACGG GACTAAAGAG GACACCGTCC
    TTAAAACCTG ATGTGCCACC AAAGCCTTCA TTTGTTCCTC AAACCACATC TGTCAGACCA
    CTGAACAAAT ACACTTACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe28814
    Clone ID Related Accession (Same CDS sequence) XM_003640050.4 , XM_003640050.3
    Accession Version XM_003640050.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product semaphorin-6D isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003640050.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAGGTTCT TCCTGCTTTG TGCCTACATG CTGCTCCTGA TGATTTCCCA GCTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTC AATACTGTTG ATTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AATGAATCCC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAGTCCCCAA GACAGAAGTA ATACCAAGCA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ACCGAGAAAA CTGTGCTATG AAGGGCAAAC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTCCC AAGAAATGAT GAGATGGTTT TTGTATGTGG TACCAATGCA
    TTCAATCCCA TGTGCAGATA CTATAGGTTG AATACCTTAG AGTATGATGG AGAGGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTAT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC CCTTCGTACA ATAAAATATG ATTCCAAATG GATAAAAGAG
    CCACATTTTC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAATAATTT AGGCAAGGCT GTATATTCCC GTGTGGCCCG CATATGTAAA
    AATGACATGG GTGGCTCGCA GCGGGTCCTG GAGAAACACT GGACTTCATT TCTGAAGGCT
    CGGCTTAACT GCTCTGTCCC CGGAGATTCG TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACGGACATCA TACAAATCAA TGGCATCCCC ACTGTGGTCG GGGTGTTTAC CACACAGCTC
    AACAGCATTC CTGGGTCTGC AGTGTGTGCA TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGCT TTAAAGAACA GAAAACCCCA GATTCTGTTT GGACAGCAGT CCCTGAAGAC
    AAAGTACCCA AGCCAAGGCC TGGCTGTTGT GCAAAGCATG GCCTTGCCGA AGCTTATAAA
    ACCTCCATAG ATTTCCCTGA CGAAACCCTG TCATTCATCA AATCCCATCC GCTAATGGAC
    GCTGCTGTAC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCTGTTGA CCACACTGCT GGGCCCCATC AGAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTGCTTAAA GTTTTGGCAA AGACCAGCCC TTTCTCTTTG
    AATGACAGTG TATTACTGGA AGAGATTGAA GCATACAACC AAGCAAAGTG CAATGCTGAG
    AATGAGGAAG ACAGAAGGGT CATCTCCTTA CAGTTGGATA AAGACCATCA CGCTGTGTAC
    GTGGCATTCT CTAGCTGCGT CATCCGCCTC CCTCTCAGCC GTTGCGAGCG TTACGGATCC
    TGTAAGAAGT CTTGTGTTGC ATCTCGGGAC CCATACTGTG GCTGGTTAAG CCAGGGGGCC
    TGTGGCAGAG TGACCCCAGG GATGCTCGCT GGAGGATATG AACAAGACAC AGAGTACGGC
    AACACGGCCC ACCTAGGGGA CTGCCATGAA ATTTTGCCTA CTTCAACTAC ACCAGATTAC
    AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCTT CATCATCTGT TACCACAATG
    GCAAGTATCC CAGAAATTAC ACCTAAAGTG ATTGATACCT GGAGGCCTAA ACTGACGAGC
    TCCCGGAAAT TTGTAGTTCA AGATGACCCA AACACTTCTG ATTTTACTGA TCCTTTATCG
    GGTATCCCAA AGGGTGTGCG ATGGGAAGTC CAATCGGGAG AGTCCAACCA GATGGTCCAC
    ATGAACGTCC TCATCACCTG TGTCTTTGCC GCTTTTGTTT TGGGTGCGTT CATTGCAGGG
    GTGGCAGTAT ACTGCTATCG TGACATGTTT GTTCGGAAAA ACAGAAAGAT TCATAAGGAT
    GCAGAATCTG CCCAGTCGTG CACAGACTCC AGTGGAAGTT TTGCCAAACT GAATGGTCTG
    TTTGATAGCC CGGTCAAGGA ATATCAACAG AATATCGATT CTCCCAAATT ATACAGTAAC
    CTGCTGACTA GTCGGAAAGA GCTGCCACCC AATGGAGATA CCAAATCCAT GGTGATGGAC
    CATCGAGGCC AACCTCCCGA ACTGGCTGCT CTCCCCACGC CTGAGTCAAC TCCTGTGCTT
    CACCAGAAGA CCCTGCAGGC CATGAAGAGC CACTCAGATA AGGCCCATGG CCATGGGGCT
    TCAAGGAAAG AAACCTCACA GTTTTTCCCA TCTAGTCCAC CACCCCATTC CCCATTAAGT
    CATGGGCATA TCCCCAGTGC CATTGTTCTT CCTAATGCTA CTCATGACTA CAACACGTCT
    TTCTCAAACT CCAATGCTCA CAAAGCTGAA AAGAAGCTTC AAAACATTGA CCACCCACTT
    ACAAAGTCAT CCAGTAAAAG AGATCACCGG CGTTCTGTGG ATTCCAGAAA TACCCTCAAT
    GATCTCCTGA AGCATTTAAA TGACCCAAAC AGCAACCCCA AAGCCATCAT GGGAGATATC
    CAGATGGCCC ACCAGACCCT AATGCTGGAT CCTGTGGGAC CTATGTCTGA GGTCCCGCCC
    AAGGTCCCTA ACCGGGAGGC ATCCCTATAC TCTCCTCCTT CAACTCTCCC CAGAAATAGC
    CCAACCAAGC GAGTGGATGT CCCCACCACT CCTGGGGTCC CAATGACTTC TCTGGAAAGA
    CAAAGGGGTT ACCACAAAAA TTCCTCCCAG AGGCACTCTA TATCTGCTAT GCCTAAAAAC
    TTAAACTCAC CAAATGGTGT TTTGTTATCC AGACAGCCTA GCATGAACCG TGGTGGGTAT
    GTGCCCACCT CCACTGGGGC AAAGGTGGAC TATATTCAGG GAGCACCAGT GAGTGTTCAT
    CTGCAGCCTT CCCTCTCCAG ACAGAGCAGC TATACCAGTA ATGGCACCCT TCCTAGGACG
    GGACTAAAGA GGACACCGTC CTTAAAACCT GATGTGCCAC CAAAGCCTTC ATTTGTTCCT
    CAAACCACAT CTGTCAGACC ACTGAACAAA TACACTTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003640098.1
    CDS287..3508
    Translation

    Target ORF information:

    RefSeq Version XM_003640050.4
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris semaphorin 6D (SEMA6D), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003640050.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAGGTTCT TCCTGCTTTG TGCCTACATG CTGCTCCTGA TGATTTCCCA GCTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTC AATACTGTTG ATTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AATGAATCCC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAGTCCCCAA GACAGAAGTA ATACCAAGCA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ACCGAGAAAA CTGTGCTATG AAGGGCAAAC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTCCC AAGAAATGAT GAGATGGTTT TTGTATGTGG TACCAATGCA
    TTCAATCCCA TGTGCAGATA CTATAGGTTG AATACCTTAG AGTATGATGG AGAGGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTAT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC CCTTCGTACA ATAAAATATG ATTCCAAATG GATAAAAGAG
    CCACATTTTC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAATAATTT AGGCAAGGCT GTATATTCCC GTGTGGCCCG CATATGTAAA
    AATGACATGG GTGGCTCGCA GCGGGTCCTG GAGAAACACT GGACTTCATT TCTGAAGGCT
    CGGCTTAACT GCTCTGTCCC CGGAGATTCG TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACGGACATCA TACAAATCAA TGGCATCCCC ACTGTGGTCG GGGTGTTTAC CACACAGCTC
    AACAGCATTC CTGGGTCTGC AGTGTGTGCA TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGCT TTAAAGAACA GAAAACCCCA GATTCTGTTT GGACAGCAGT CCCTGAAGAC
    AAAGTACCCA AGCCAAGGCC TGGCTGTTGT GCAAAGCATG GCCTTGCCGA AGCTTATAAA
    ACCTCCATAG ATTTCCCTGA CGAAACCCTG TCATTCATCA AATCCCATCC GCTAATGGAC
    GCTGCTGTAC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCTGTTGA CCACACTGCT GGGCCCCATC AGAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTGCTTAAA GTTTTGGCAA AGACCAGCCC TTTCTCTTTG
    AATGACAGTG TATTACTGGA AGAGATTGAA GCATACAACC AAGCAAAGTG CAATGCTGAG
    AATGAGGAAG ACAGAAGGGT CATCTCCTTA CAGTTGGATA AAGACCATCA CGCTGTGTAC
    GTGGCATTCT CTAGCTGCGT CATCCGCCTC CCTCTCAGCC GTTGCGAGCG TTACGGATCC
    TGTAAGAAGT CTTGTGTTGC ATCTCGGGAC CCATACTGTG GCTGGTTAAG CCAGGGGGCC
    TGTGGCAGAG TGACCCCAGG GATGCTCGCT GGAGGATATG AACAAGACAC AGAGTACGGC
    AACACGGCCC ACCTAGGGGA CTGCCATGAA ATTTTGCCTA CTTCAACTAC ACCAGATTAC
    AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCTT CATCATCTGT TACCACAATG
    GCAAGTATCC CAGAAATTAC ACCTAAAGTG ATTGATACCT GGAGGCCTAA ACTGACGAGC
    TCCCGGAAAT TTGTAGTTCA AGATGACCCA AACACTTCTG ATTTTACTGA TCCTTTATCG
    GGTATCCCAA AGGGTGTGCG ATGGGAAGTC CAATCGGGAG AGTCCAACCA GATGGTCCAC
    ATGAACGTCC TCATCACCTG TGTCTTTGCC GCTTTTGTTT TGGGTGCGTT CATTGCAGGG
    GTGGCAGTAT ACTGCTATCG TGACATGTTT GTTCGGAAAA ACAGAAAGAT TCATAAGGAT
    GCAGAATCTG CCCAGTCGTG CACAGACTCC AGTGGAAGTT TTGCCAAACT GAATGGTCTG
    TTTGATAGCC CGGTCAAGGA ATATCAACAG AATATCGATT CTCCCAAATT ATACAGTAAC
    CTGCTGACTA GTCGGAAAGA GCTGCCACCC AATGGAGATA CCAAATCCAT GGTGATGGAC
    CATCGAGGCC AACCTCCCGA ACTGGCTGCT CTCCCCACGC CTGAGTCAAC TCCTGTGCTT
    CACCAGAAGA CCCTGCAGGC CATGAAGAGC CACTCAGATA AGGCCCATGG CCATGGGGCT
    TCAAGGAAAG AAACCTCACA GTTTTTCCCA TCTAGTCCAC CACCCCATTC CCCATTAAGT
    CATGGGCATA TCCCCAGTGC CATTGTTCTT CCTAATGCTA CTCATGACTA CAACACGTCT
    TTCTCAAACT CCAATGCTCA CAAAGCTGAA AAGAAGCTTC AAAACATTGA CCACCCACTT
    ACAAAGTCAT CCAGTAAAAG AGATCACCGG CGTTCTGTGG ATTCCAGAAA TACCCTCAAT
    GATCTCCTGA AGCATTTAAA TGACCCAAAC AGCAACCCCA AAGCCATCAT GGGAGATATC
    CAGATGGCCC ACCAGACCCT AATGCTGGAT CCTGTGGGAC CTATGTCTGA GGTCCCGCCC
    AAGGTCCCTA ACCGGGAGGC ATCCCTATAC TCTCCTCCTT CAACTCTCCC CAGAAATAGC
    CCAACCAAGC GAGTGGATGT CCCCACCACT CCTGGGGTCC CAATGACTTC TCTGGAAAGA
    CAAAGGGGTT ACCACAAAAA TTCCTCCCAG AGGCACTCTA TATCTGCTAT GCCTAAAAAC
    TTAAACTCAC CAAATGGTGT TTTGTTATCC AGACAGCCTA GCATGAACCG TGGTGGGTAT
    GTGCCCACCT CCACTGGGGC AAAGGTGGAC TATATTCAGG GAGCACCAGT GAGTGTTCAT
    CTGCAGCCTT CCCTCTCCAG ACAGAGCAGC TATACCAGTA ATGGCACCCT TCCTAGGACG
    GGACTAAAGA GGACACCGTC CTTAAAACCT GATGTGCCAC CAAAGCCTTC ATTTGTTCCT
    CAAACCACAT CTGTCAGACC ACTGAACAAA TACACTTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe28814
    Clone ID Related Accession (Same CDS sequence) XM_003640050.4 , XM_003640050.3
    Accession Version XM_003640050.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product semaphorin-6D isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003640050.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAGGTTCT TCCTGCTTTG TGCCTACATG CTGCTCCTGA TGATTTCCCA GCTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTC AATACTGTTG ATTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AATGAATCCC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAGTCCCCAA GACAGAAGTA ATACCAAGCA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ACCGAGAAAA CTGTGCTATG AAGGGCAAAC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTCCC AAGAAATGAT GAGATGGTTT TTGTATGTGG TACCAATGCA
    TTCAATCCCA TGTGCAGATA CTATAGGTTG AATACCTTAG AGTATGATGG AGAGGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTAT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC CCTTCGTACA ATAAAATATG ATTCCAAATG GATAAAAGAG
    CCACATTTTC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAATAATTT AGGCAAGGCT GTATATTCCC GTGTGGCCCG CATATGTAAA
    AATGACATGG GTGGCTCGCA GCGGGTCCTG GAGAAACACT GGACTTCATT TCTGAAGGCT
    CGGCTTAACT GCTCTGTCCC CGGAGATTCG TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACGGACATCA TACAAATCAA TGGCATCCCC ACTGTGGTCG GGGTGTTTAC CACACAGCTC
    AACAGCATTC CTGGGTCTGC AGTGTGTGCA TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGCT TTAAAGAACA GAAAACCCCA GATTCTGTTT GGACAGCAGT CCCTGAAGAC
    AAAGTACCCA AGCCAAGGCC TGGCTGTTGT GCAAAGCATG GCCTTGCCGA AGCTTATAAA
    ACCTCCATAG ATTTCCCTGA CGAAACCCTG TCATTCATCA AATCCCATCC GCTAATGGAC
    GCTGCTGTAC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCTGTTGA CCACACTGCT GGGCCCCATC AGAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTGCTTAAA GTTTTGGCAA AGACCAGCCC TTTCTCTTTG
    AATGACAGTG TATTACTGGA AGAGATTGAA GCATACAACC AAGCAAAGTG CAATGCTGAG
    AATGAGGAAG ACAGAAGGGT CATCTCCTTA CAGTTGGATA AAGACCATCA CGCTGTGTAC
    GTGGCATTCT CTAGCTGCGT CATCCGCCTC CCTCTCAGCC GTTGCGAGCG TTACGGATCC
    TGTAAGAAGT CTTGTGTTGC ATCTCGGGAC CCATACTGTG GCTGGTTAAG CCAGGGGGCC
    TGTGGCAGAG TGACCCCAGG GATGCTCGCT GGAGGATATG AACAAGACAC AGAGTACGGC
    AACACGGCCC ACCTAGGGGA CTGCCATGAA ATTTTGCCTA CTTCAACTAC ACCAGATTAC
    AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCTT CATCATCTGT TACCACAATG
    GCAAGTATCC CAGAAATTAC ACCTAAAGTG ATTGATACCT GGAGGCCTAA ACTGACGAGC
    TCCCGGAAAT TTGTAGTTCA AGATGACCCA AACACTTCTG ATTTTACTGA TCCTTTATCG
    GGTATCCCAA AGGGTGTGCG ATGGGAAGTC CAATCGGGAG AGTCCAACCA GATGGTCCAC
    ATGAACGTCC TCATCACCTG TGTCTTTGCC GCTTTTGTTT TGGGTGCGTT CATTGCAGGG
    GTGGCAGTAT ACTGCTATCG TGACATGTTT GTTCGGAAAA ACAGAAAGAT TCATAAGGAT
    GCAGAATCTG CCCAGTCGTG CACAGACTCC AGTGGAAGTT TTGCCAAACT GAATGGTCTG
    TTTGATAGCC CGGTCAAGGA ATATCAACAG AATATCGATT CTCCCAAATT ATACAGTAAC
    CTGCTGACTA GTCGGAAAGA GCTGCCACCC AATGGAGATA CCAAATCCAT GGTGATGGAC
    CATCGAGGCC AACCTCCCGA ACTGGCTGCT CTCCCCACGC CTGAGTCAAC TCCTGTGCTT
    CACCAGAAGA CCCTGCAGGC CATGAAGAGC CACTCAGATA AGGCCCATGG CCATGGGGCT
    TCAAGGAAAG AAACCTCACA GTTTTTCCCA TCTAGTCCAC CACCCCATTC CCCATTAAGT
    CATGGGCATA TCCCCAGTGC CATTGTTCTT CCTAATGCTA CTCATGACTA CAACACGTCT
    TTCTCAAACT CCAATGCTCA CAAAGCTGAA AAGAAGCTTC AAAACATTGA CCACCCACTT
    ACAAAGTCAT CCAGTAAAAG AGATCACCGG CGTTCTGTGG ATTCCAGAAA TACCCTCAAT
    GATCTCCTGA AGCATTTAAA TGACCCAAAC AGCAACCCCA AAGCCATCAT GGGAGATATC
    CAGATGGCCC ACCAGACCCT AATGCTGGAT CCTGTGGGAC CTATGTCTGA GGTCCCGCCC
    AAGGTCCCTA ACCGGGAGGC ATCCCTATAC TCTCCTCCTT CAACTCTCCC CAGAAATAGC
    CCAACCAAGC GAGTGGATGT CCCCACCACT CCTGGGGTCC CAATGACTTC TCTGGAAAGA
    CAAAGGGGTT ACCACAAAAA TTCCTCCCAG AGGCACTCTA TATCTGCTAT GCCTAAAAAC
    TTAAACTCAC CAAATGGTGT TTTGTTATCC AGACAGCCTA GCATGAACCG TGGTGGGTAT
    GTGCCCACCT CCACTGGGGC AAAGGTGGAC TATATTCAGG GAGCACCAGT GAGTGTTCAT
    CTGCAGCCTT CCCTCTCCAG ACAGAGCAGC TATACCAGTA ATGGCACCCT TCCTAGGACG
    GGACTAAAGA GGACACCGTC CTTAAAACCT GATGTGCCAC CAAAGCCTTC ATTTGTTCCT
    CAAACCACAT CTGTCAGACC ACTGAACAAA TACACTTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003640098.1
    CDS314..3535
    Translation

    Target ORF information:

    RefSeq Version XM_003640050.3
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (SEMA6D), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003640050.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAGGTTCT TCCTGCTTTG TGCCTACATG CTGCTCCTGA TGATTTCCCA GCTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTC AATACTGTTG ATTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AATGAATCCC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAGTCCCCAA GACAGAAGTA ATACCAAGCA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ACCGAGAAAA CTGTGCTATG AAGGGCAAAC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTCCC AAGAAATGAT GAGATGGTTT TTGTATGTGG TACCAATGCA
    TTCAATCCCA TGTGCAGATA CTATAGGTTG AATACCTTAG AGTATGATGG AGAGGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTAT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC CCTTCGTACA ATAAAATATG ATTCCAAATG GATAAAAGAG
    CCACATTTTC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAATAATTT AGGCAAGGCT GTATATTCCC GTGTGGCCCG CATATGTAAA
    AATGACATGG GTGGCTCGCA GCGGGTCCTG GAGAAACACT GGACTTCATT TCTGAAGGCT
    CGGCTTAACT GCTCTGTCCC CGGAGATTCG TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACGGACATCA TACAAATCAA TGGCATCCCC ACTGTGGTCG GGGTGTTTAC CACACAGCTC
    AACAGCATTC CTGGGTCTGC AGTGTGTGCA TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGCT TTAAAGAACA GAAAACCCCA GATTCTGTTT GGACAGCAGT CCCTGAAGAC
    AAAGTACCCA AGCCAAGGCC TGGCTGTTGT GCAAAGCATG GCCTTGCCGA AGCTTATAAA
    ACCTCCATAG ATTTCCCTGA CGAAACCCTG TCATTCATCA AATCCCATCC GCTAATGGAC
    GCTGCTGTAC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCTGTTGA CCACACTGCT GGGCCCCATC AGAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTGCTTAAA GTTTTGGCAA AGACCAGCCC TTTCTCTTTG
    AATGACAGTG TATTACTGGA AGAGATTGAA GCATACAACC AAGCAAAGTG CAATGCTGAG
    AATGAGGAAG ACAGAAGGGT CATCTCCTTA CAGTTGGATA AAGACCATCA CGCTGTGTAC
    GTGGCATTCT CTAGCTGCGT CATCCGCCTC CCTCTCAGCC GTTGCGAGCG TTACGGATCC
    TGTAAGAAGT CTTGTGTTGC ATCTCGGGAC CCATACTGTG GCTGGTTAAG CCAGGGGGCC
    TGTGGCAGAG TGACCCCAGG GATGCTCGCT GGAGGATATG AACAAGACAC AGAGTACGGC
    AACACGGCCC ACCTAGGGGA CTGCCATGAA ATTTTGCCTA CTTCAACTAC ACCAGATTAC
    AAAATATTTG GCGGTCCAAC ATCTGACATG GAGGTATCTT CATCATCTGT TACCACAATG
    GCAAGTATCC CAGAAATTAC ACCTAAAGTG ATTGATACCT GGAGGCCTAA ACTGACGAGC
    TCCCGGAAAT TTGTAGTTCA AGATGACCCA AACACTTCTG ATTTTACTGA TCCTTTATCG
    GGTATCCCAA AGGGTGTGCG ATGGGAAGTC CAATCGGGAG AGTCCAACCA GATGGTCCAC
    ATGAACGTCC TCATCACCTG TGTCTTTGCC GCTTTTGTTT TGGGTGCGTT CATTGCAGGG
    GTGGCAGTAT ACTGCTATCG TGACATGTTT GTTCGGAAAA ACAGAAAGAT TCATAAGGAT
    GCAGAATCTG CCCAGTCGTG CACAGACTCC AGTGGAAGTT TTGCCAAACT GAATGGTCTG
    TTTGATAGCC CGGTCAAGGA ATATCAACAG AATATCGATT CTCCCAAATT ATACAGTAAC
    CTGCTGACTA GTCGGAAAGA GCTGCCACCC AATGGAGATA CCAAATCCAT GGTGATGGAC
    CATCGAGGCC AACCTCCCGA ACTGGCTGCT CTCCCCACGC CTGAGTCAAC TCCTGTGCTT
    CACCAGAAGA CCCTGCAGGC CATGAAGAGC CACTCAGATA AGGCCCATGG CCATGGGGCT
    TCAAGGAAAG AAACCTCACA GTTTTTCCCA TCTAGTCCAC CACCCCATTC CCCATTAAGT
    CATGGGCATA TCCCCAGTGC CATTGTTCTT CCTAATGCTA CTCATGACTA CAACACGTCT
    TTCTCAAACT CCAATGCTCA CAAAGCTGAA AAGAAGCTTC AAAACATTGA CCACCCACTT
    ACAAAGTCAT CCAGTAAAAG AGATCACCGG CGTTCTGTGG ATTCCAGAAA TACCCTCAAT
    GATCTCCTGA AGCATTTAAA TGACCCAAAC AGCAACCCCA AAGCCATCAT GGGAGATATC
    CAGATGGCCC ACCAGACCCT AATGCTGGAT CCTGTGGGAC CTATGTCTGA GGTCCCGCCC
    AAGGTCCCTA ACCGGGAGGC ATCCCTATAC TCTCCTCCTT CAACTCTCCC CAGAAATAGC
    CCAACCAAGC GAGTGGATGT CCCCACCACT CCTGGGGTCC CAATGACTTC TCTGGAAAGA
    CAAAGGGGTT ACCACAAAAA TTCCTCCCAG AGGCACTCTA TATCTGCTAT GCCTAAAAAC
    TTAAACTCAC CAAATGGTGT TTTGTTATCC AGACAGCCTA GCATGAACCG TGGTGGGTAT
    GTGCCCACCT CCACTGGGGC AAAGGTGGAC TATATTCAGG GAGCACCAGT GAGTGTTCAT
    CTGCAGCCTT CCCTCTCCAG ACAGAGCAGC TATACCAGTA ATGGCACCCT TCCTAGGACG
    GGACTAAAGA GGACACCGTC CTTAAAACCT GATGTGCCAC CAAAGCCTTC ATTTGTTCCT
    CAAACCACAT CTGTCAGACC ACTGAACAAA TACACTTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe28815
    Clone ID Related Accession (Same CDS sequence) XM_005638383.2 , XM_005638383.3
    Accession Version XM_005638383.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3204bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product semaphorin-6D isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005638383.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAGGTTCT TCCTGCTTTG TGCCTACATG CTGCTCCTGA TGATTTCCCA GCTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTC AATACTGTTG ATTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AATGAATCCC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAGTCCCCAA GACAGAAGTA ATACCAAGCA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ACCGAGAAAA CTGTGCTATG AAGGGCAAAC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTCCC AAGAAATGAT GAGATGGTTT TTGTATGTGG TACCAATGCA
    TTCAATCCCA TGTGCAGATA CTATAGGTTG AATACCTTAG AGTATGATGG AGAGGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTAT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC CCTTCGTACA ATAAAATATG ATTCCAAATG GATAAAAGAG
    CCACATTTTC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAATAATTT AGGCAAGGCT GTATATTCCC GTGTGGCCCG CATATGTAAA
    AATGACATGG GTGGCTCGCA GCGGGTCCTG GAGAAACACT GGACTTCATT TCTGAAGGCT
    CGGCTTAACT GCTCTGTCCC CGGAGATTCG TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACGGACATCA TACAAATCAA TGGCATCCCC ACTGTGGTCG GGGTGTTTAC CACACAGCTC
    AACAGCATTC CTGGGTCTGC AGTGTGTGCA TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGCT TTAAAGAACA GAAAACCCCA GATTCTGTTT GGACAGCAGT CCCTGAAGAC
    AAAGTACCCA AGCCAAGGCC TGGCTGTTGT GCAAAGCATG GCCTTGCCGA AGCTTATAAA
    ACCTCCATAG ATTTCCCTGA CGAAACCCTG TCATTCATCA AATCCCATCC GCTAATGGAC
    GCTGCTGTAC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCTGTTGA CCACACTGCT GGGCCCCATC AGAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTGCTTAAA GTTTTGGCAA AGACCAGCCC TTTCTCTTTG
    AATGACAGTG TATTACTGGA AGAGATTGAA GCATACAACC AAGCAAAGTG CAATGCTGAG
    AATGAGGAAG ACAGAAGGGT CATCTCCTTA CAGTTGGATA AAGACCATCA CGCTGTGTAC
    GTGGCATTCT CTAGCTGCGT CATCCGCCTC CCTCTCAGCC GTTGCGAGCG TTACGGATCC
    TGTAAGAAGT CTTGTGTTGC ATCTCGGGAC CCATACTGTG GCTGGTTAAG CCAGGGGGCC
    TGTGGCAGAG TGACCCCAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATATGA ACAAGACACA GAGTACGGCA ACACGGCCCA CCTAGGGGAC
    TGCCATGACA TGGAGGTATC TTCATCATCT GTTACCACAA TGGCAAGTAT CCCAGAAATT
    ACACCTAAAG TGATTGATAC CTGGAGGCCT AAACTGACGA GCTCCCGGAA ATTTGTAGTT
    CAAGATGACC CAAACACTTC TGATTTTACT GATCCTTTAT CGGGTATCCC AAAGGGTGTG
    CGATGGGAAG TCCAATCGGG AGAGTCCAAC CAGATGGTCC ACATGAACGT CCTCATCACC
    TGTGTCTTTG CCGCTTTTGT TTTGGGTGCG TTCATTGCAG GGGTGGCAGT ATACTGCTAT
    CGTGACATGT TTGTTCGGAA AAACAGAAAG ATTCATAAGG ATGCAGAATC TGCCCAGTCG
    TGCACAGACT CCAGTGGAAG TTTTGCCAAA CTGAATGGTC TGTTTGATAG CCCGGTCAAG
    GAATATCAAC AGAATATCGA TTCTCCCAAA TTATACAGTA ACCTGCTGAC TAGTCGGAAA
    GAGCTGCCAC CCAATGGAGA TACCAAATCC ATGGTGATGG ACCATCGAGG CCAACCTCCC
    GAACTGGCTG CTCTCCCCAC GCCTGAGTCA ACTCCTGTGC TTCACCAGAA GACCCTGCAG
    GCCATGAAGA GCCACTCAGA TAAGGCCCAT GGCCATGGGG CTTCAAGGAA AGAAACCTCA
    CAGTTTTTCC CATCTAGTCC ACCACCCCAT TCCCCATTAA GTCATGGGCA TATCCCCAGT
    GCCATTGTTC TTCCTAATGC TACTCATGAC TACAACACGT CTTTCTCAAA CTCCAATGCT
    CACAAAGCTG AAAAGAAGCT TCAAAACATT GACCACCCAC TTACAAAGTC ATCCAGTAAA
    AGAGATCACC GGCGTTCTGT GGATTCCAGA AATACCCTCA ATGATCTCCT GAAGCATTTA
    AATGACCCAA ACAGCAACCC CAAAGCCATC ATGGGAGATA TCCAGATGGC CCACCAGACC
    CTAATGCTGG ATCCTGTGGG ACCTATGTCT GAGGTCCCGC CCAAGGTCCC TAACCGGGAG
    GCATCCCTAT ACTCTCCTCC TTCAACTCTC CCCAGAAATA GCCCAACCAA GCGAGTGGAT
    GTCCCCACCA CTCCTGGGGT CCCAATGACT TCTCTGGAAA GACAAAGGGG TTACCACAAA
    AATTCCTCCC AGAGGCACTC TATATCTGCT ATGCCTAAAA ACTTAAACTC ACCAAATGGT
    GTTTTGTTAT CCAGACAGCC TAGCATGAAC CGTGGTGGGT ATGTGCCCAC CTCCACTGGG
    GCAAAGGTGG ACTATATTCA GGGAGCACCA GTGAGTGTTC ATCTGCAGCC TTCCCTCTCC
    AGACAGAGCA GCTATACCAG TAATGGCACC CTTCCTAGGA CGGGACTAAA GAGGACACCG
    TCCTTAAAAC CTGATGTGCC ACCAAAGCCT TCATTTGTTC CTCAAACCAC ATCTGTCAGA
    CCACTGAACA AATACACTTA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005638440.1
    CDS314..3517
    Translation

    Target ORF information:

    RefSeq Version XM_005638383.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (SEMA6D), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005638383.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAGGTTCT TCCTGCTTTG TGCCTACATG CTGCTCCTGA TGATTTCCCA GCTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTC AATACTGTTG ATTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AATGAATCCC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAGTCCCCAA GACAGAAGTA ATACCAAGCA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ACCGAGAAAA CTGTGCTATG AAGGGCAAAC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTCCC AAGAAATGAT GAGATGGTTT TTGTATGTGG TACCAATGCA
    TTCAATCCCA TGTGCAGATA CTATAGGTTG AATACCTTAG AGTATGATGG AGAGGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTAT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC CCTTCGTACA ATAAAATATG ATTCCAAATG GATAAAAGAG
    CCACATTTTC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAATAATTT AGGCAAGGCT GTATATTCCC GTGTGGCCCG CATATGTAAA
    AATGACATGG GTGGCTCGCA GCGGGTCCTG GAGAAACACT GGACTTCATT TCTGAAGGCT
    CGGCTTAACT GCTCTGTCCC CGGAGATTCG TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACGGACATCA TACAAATCAA TGGCATCCCC ACTGTGGTCG GGGTGTTTAC CACACAGCTC
    AACAGCATTC CTGGGTCTGC AGTGTGTGCA TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGCT TTAAAGAACA GAAAACCCCA GATTCTGTTT GGACAGCAGT CCCTGAAGAC
    AAAGTACCCA AGCCAAGGCC TGGCTGTTGT GCAAAGCATG GCCTTGCCGA AGCTTATAAA
    ACCTCCATAG ATTTCCCTGA CGAAACCCTG TCATTCATCA AATCCCATCC GCTAATGGAC
    GCTGCTGTAC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCTGTTGA CCACACTGCT GGGCCCCATC AGAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTGCTTAAA GTTTTGGCAA AGACCAGCCC TTTCTCTTTG
    AATGACAGTG TATTACTGGA AGAGATTGAA GCATACAACC AAGCAAAGTG CAATGCTGAG
    AATGAGGAAG ACAGAAGGGT CATCTCCTTA CAGTTGGATA AAGACCATCA CGCTGTGTAC
    GTGGCATTCT CTAGCTGCGT CATCCGCCTC CCTCTCAGCC GTTGCGAGCG TTACGGATCC
    TGTAAGAAGT CTTGTGTTGC ATCTCGGGAC CCATACTGTG GCTGGTTAAG CCAGGGGGCC
    TGTGGCAGAG TGACCCCAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATATGA ACAAGACACA GAGTACGGCA ACACGGCCCA CCTAGGGGAC
    TGCCATGACA TGGAGGTATC TTCATCATCT GTTACCACAA TGGCAAGTAT CCCAGAAATT
    ACACCTAAAG TGATTGATAC CTGGAGGCCT AAACTGACGA GCTCCCGGAA ATTTGTAGTT
    CAAGATGACC CAAACACTTC TGATTTTACT GATCCTTTAT CGGGTATCCC AAAGGGTGTG
    CGATGGGAAG TCCAATCGGG AGAGTCCAAC CAGATGGTCC ACATGAACGT CCTCATCACC
    TGTGTCTTTG CCGCTTTTGT TTTGGGTGCG TTCATTGCAG GGGTGGCAGT ATACTGCTAT
    CGTGACATGT TTGTTCGGAA AAACAGAAAG ATTCATAAGG ATGCAGAATC TGCCCAGTCG
    TGCACAGACT CCAGTGGAAG TTTTGCCAAA CTGAATGGTC TGTTTGATAG CCCGGTCAAG
    GAATATCAAC AGAATATCGA TTCTCCCAAA TTATACAGTA ACCTGCTGAC TAGTCGGAAA
    GAGCTGCCAC CCAATGGAGA TACCAAATCC ATGGTGATGG ACCATCGAGG CCAACCTCCC
    GAACTGGCTG CTCTCCCCAC GCCTGAGTCA ACTCCTGTGC TTCACCAGAA GACCCTGCAG
    GCCATGAAGA GCCACTCAGA TAAGGCCCAT GGCCATGGGG CTTCAAGGAA AGAAACCTCA
    CAGTTTTTCC CATCTAGTCC ACCACCCCAT TCCCCATTAA GTCATGGGCA TATCCCCAGT
    GCCATTGTTC TTCCTAATGC TACTCATGAC TACAACACGT CTTTCTCAAA CTCCAATGCT
    CACAAAGCTG AAAAGAAGCT TCAAAACATT GACCACCCAC TTACAAAGTC ATCCAGTAAA
    AGAGATCACC GGCGTTCTGT GGATTCCAGA AATACCCTCA ATGATCTCCT GAAGCATTTA
    AATGACCCAA ACAGCAACCC CAAAGCCATC ATGGGAGATA TCCAGATGGC CCACCAGACC
    CTAATGCTGG ATCCTGTGGG ACCTATGTCT GAGGTCCCGC CCAAGGTCCC TAACCGGGAG
    GCATCCCTAT ACTCTCCTCC TTCAACTCTC CCCAGAAATA GCCCAACCAA GCGAGTGGAT
    GTCCCCACCA CTCCTGGGGT CCCAATGACT TCTCTGGAAA GACAAAGGGG TTACCACAAA
    AATTCCTCCC AGAGGCACTC TATATCTGCT ATGCCTAAAA ACTTAAACTC ACCAAATGGT
    GTTTTGTTAT CCAGACAGCC TAGCATGAAC CGTGGTGGGT ATGTGCCCAC CTCCACTGGG
    GCAAAGGTGG ACTATATTCA GGGAGCACCA GTGAGTGTTC ATCTGCAGCC TTCCCTCTCC
    AGACAGAGCA GCTATACCAG TAATGGCACC CTTCCTAGGA CGGGACTAAA GAGGACACCG
    TCCTTAAAAC CTGATGTGCC ACCAAAGCCT TCATTTGTTC CTCAAACCAC ATCTGTCAGA
    CCACTGAACA AATACACTTA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe28815
    Clone ID Related Accession (Same CDS sequence) XM_005638383.2 , XM_005638383.3
    Accession Version XM_005638383.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3204bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product semaphorin-6D isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005638383.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAGGTTCT TCCTGCTTTG TGCCTACATG CTGCTCCTGA TGATTTCCCA GCTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTC AATACTGTTG ATTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AATGAATCCC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAGTCCCCAA GACAGAAGTA ATACCAAGCA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ACCGAGAAAA CTGTGCTATG AAGGGCAAAC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTCCC AAGAAATGAT GAGATGGTTT TTGTATGTGG TACCAATGCA
    TTCAATCCCA TGTGCAGATA CTATAGGTTG AATACCTTAG AGTATGATGG AGAGGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTAT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC CCTTCGTACA ATAAAATATG ATTCCAAATG GATAAAAGAG
    CCACATTTTC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAATAATTT AGGCAAGGCT GTATATTCCC GTGTGGCCCG CATATGTAAA
    AATGACATGG GTGGCTCGCA GCGGGTCCTG GAGAAACACT GGACTTCATT TCTGAAGGCT
    CGGCTTAACT GCTCTGTCCC CGGAGATTCG TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACGGACATCA TACAAATCAA TGGCATCCCC ACTGTGGTCG GGGTGTTTAC CACACAGCTC
    AACAGCATTC CTGGGTCTGC AGTGTGTGCA TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGCT TTAAAGAACA GAAAACCCCA GATTCTGTTT GGACAGCAGT CCCTGAAGAC
    AAAGTACCCA AGCCAAGGCC TGGCTGTTGT GCAAAGCATG GCCTTGCCGA AGCTTATAAA
    ACCTCCATAG ATTTCCCTGA CGAAACCCTG TCATTCATCA AATCCCATCC GCTAATGGAC
    GCTGCTGTAC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCTGTTGA CCACACTGCT GGGCCCCATC AGAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTGCTTAAA GTTTTGGCAA AGACCAGCCC TTTCTCTTTG
    AATGACAGTG TATTACTGGA AGAGATTGAA GCATACAACC AAGCAAAGTG CAATGCTGAG
    AATGAGGAAG ACAGAAGGGT CATCTCCTTA CAGTTGGATA AAGACCATCA CGCTGTGTAC
    GTGGCATTCT CTAGCTGCGT CATCCGCCTC CCTCTCAGCC GTTGCGAGCG TTACGGATCC
    TGTAAGAAGT CTTGTGTTGC ATCTCGGGAC CCATACTGTG GCTGGTTAAG CCAGGGGGCC
    TGTGGCAGAG TGACCCCAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATATGA ACAAGACACA GAGTACGGCA ACACGGCCCA CCTAGGGGAC
    TGCCATGACA TGGAGGTATC TTCATCATCT GTTACCACAA TGGCAAGTAT CCCAGAAATT
    ACACCTAAAG TGATTGATAC CTGGAGGCCT AAACTGACGA GCTCCCGGAA ATTTGTAGTT
    CAAGATGACC CAAACACTTC TGATTTTACT GATCCTTTAT CGGGTATCCC AAAGGGTGTG
    CGATGGGAAG TCCAATCGGG AGAGTCCAAC CAGATGGTCC ACATGAACGT CCTCATCACC
    TGTGTCTTTG CCGCTTTTGT TTTGGGTGCG TTCATTGCAG GGGTGGCAGT ATACTGCTAT
    CGTGACATGT TTGTTCGGAA AAACAGAAAG ATTCATAAGG ATGCAGAATC TGCCCAGTCG
    TGCACAGACT CCAGTGGAAG TTTTGCCAAA CTGAATGGTC TGTTTGATAG CCCGGTCAAG
    GAATATCAAC AGAATATCGA TTCTCCCAAA TTATACAGTA ACCTGCTGAC TAGTCGGAAA
    GAGCTGCCAC CCAATGGAGA TACCAAATCC ATGGTGATGG ACCATCGAGG CCAACCTCCC
    GAACTGGCTG CTCTCCCCAC GCCTGAGTCA ACTCCTGTGC TTCACCAGAA GACCCTGCAG
    GCCATGAAGA GCCACTCAGA TAAGGCCCAT GGCCATGGGG CTTCAAGGAA AGAAACCTCA
    CAGTTTTTCC CATCTAGTCC ACCACCCCAT TCCCCATTAA GTCATGGGCA TATCCCCAGT
    GCCATTGTTC TTCCTAATGC TACTCATGAC TACAACACGT CTTTCTCAAA CTCCAATGCT
    CACAAAGCTG AAAAGAAGCT TCAAAACATT GACCACCCAC TTACAAAGTC ATCCAGTAAA
    AGAGATCACC GGCGTTCTGT GGATTCCAGA AATACCCTCA ATGATCTCCT GAAGCATTTA
    AATGACCCAA ACAGCAACCC CAAAGCCATC ATGGGAGATA TCCAGATGGC CCACCAGACC
    CTAATGCTGG ATCCTGTGGG ACCTATGTCT GAGGTCCCGC CCAAGGTCCC TAACCGGGAG
    GCATCCCTAT ACTCTCCTCC TTCAACTCTC CCCAGAAATA GCCCAACCAA GCGAGTGGAT
    GTCCCCACCA CTCCTGGGGT CCCAATGACT TCTCTGGAAA GACAAAGGGG TTACCACAAA
    AATTCCTCCC AGAGGCACTC TATATCTGCT ATGCCTAAAA ACTTAAACTC ACCAAATGGT
    GTTTTGTTAT CCAGACAGCC TAGCATGAAC CGTGGTGGGT ATGTGCCCAC CTCCACTGGG
    GCAAAGGTGG ACTATATTCA GGGAGCACCA GTGAGTGTTC ATCTGCAGCC TTCCCTCTCC
    AGACAGAGCA GCTATACCAG TAATGGCACC CTTCCTAGGA CGGGACTAAA GAGGACACCG
    TCCTTAAAAC CTGATGTGCC ACCAAAGCCT TCATTTGTTC CTCAAACCAC ATCTGTCAGA
    CCACTGAACA AATACACTTA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005638440.1
    CDS287..3490
    Translation

    Target ORF information:

    RefSeq Version XM_005638383.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris semaphorin 6D (SEMA6D), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005638383.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAGGTTCT TCCTGCTTTG TGCCTACATG CTGCTCCTGA TGATTTCCCA GCTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTC AATACTGTTG ATTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AATGAATCCC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAGTCCCCAA GACAGAAGTA ATACCAAGCA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ACCGAGAAAA CTGTGCTATG AAGGGCAAAC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTCCC AAGAAATGAT GAGATGGTTT TTGTATGTGG TACCAATGCA
    TTCAATCCCA TGTGCAGATA CTATAGGTTG AATACCTTAG AGTATGATGG AGAGGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTAT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC CCTTCGTACA ATAAAATATG ATTCCAAATG GATAAAAGAG
    CCACATTTTC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAATAATTT AGGCAAGGCT GTATATTCCC GTGTGGCCCG CATATGTAAA
    AATGACATGG GTGGCTCGCA GCGGGTCCTG GAGAAACACT GGACTTCATT TCTGAAGGCT
    CGGCTTAACT GCTCTGTCCC CGGAGATTCG TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACGGACATCA TACAAATCAA TGGCATCCCC ACTGTGGTCG GGGTGTTTAC CACACAGCTC
    AACAGCATTC CTGGGTCTGC AGTGTGTGCA TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGCT TTAAAGAACA GAAAACCCCA GATTCTGTTT GGACAGCAGT CCCTGAAGAC
    AAAGTACCCA AGCCAAGGCC TGGCTGTTGT GCAAAGCATG GCCTTGCCGA AGCTTATAAA
    ACCTCCATAG ATTTCCCTGA CGAAACCCTG TCATTCATCA AATCCCATCC GCTAATGGAC
    GCTGCTGTAC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCTGTTGA CCACACTGCT GGGCCCCATC AGAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTGCTTAAA GTTTTGGCAA AGACCAGCCC TTTCTCTTTG
    AATGACAGTG TATTACTGGA AGAGATTGAA GCATACAACC AAGCAAAGTG CAATGCTGAG
    AATGAGGAAG ACAGAAGGGT CATCTCCTTA CAGTTGGATA AAGACCATCA CGCTGTGTAC
    GTGGCATTCT CTAGCTGCGT CATCCGCCTC CCTCTCAGCC GTTGCGAGCG TTACGGATCC
    TGTAAGAAGT CTTGTGTTGC ATCTCGGGAC CCATACTGTG GCTGGTTAAG CCAGGGGGCC
    TGTGGCAGAG TGACCCCAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATATGA ACAAGACACA GAGTACGGCA ACACGGCCCA CCTAGGGGAC
    TGCCATGACA TGGAGGTATC TTCATCATCT GTTACCACAA TGGCAAGTAT CCCAGAAATT
    ACACCTAAAG TGATTGATAC CTGGAGGCCT AAACTGACGA GCTCCCGGAA ATTTGTAGTT
    CAAGATGACC CAAACACTTC TGATTTTACT GATCCTTTAT CGGGTATCCC AAAGGGTGTG
    CGATGGGAAG TCCAATCGGG AGAGTCCAAC CAGATGGTCC ACATGAACGT CCTCATCACC
    TGTGTCTTTG CCGCTTTTGT TTTGGGTGCG TTCATTGCAG GGGTGGCAGT ATACTGCTAT
    CGTGACATGT TTGTTCGGAA AAACAGAAAG ATTCATAAGG ATGCAGAATC TGCCCAGTCG
    TGCACAGACT CCAGTGGAAG TTTTGCCAAA CTGAATGGTC TGTTTGATAG CCCGGTCAAG
    GAATATCAAC AGAATATCGA TTCTCCCAAA TTATACAGTA ACCTGCTGAC TAGTCGGAAA
    GAGCTGCCAC CCAATGGAGA TACCAAATCC ATGGTGATGG ACCATCGAGG CCAACCTCCC
    GAACTGGCTG CTCTCCCCAC GCCTGAGTCA ACTCCTGTGC TTCACCAGAA GACCCTGCAG
    GCCATGAAGA GCCACTCAGA TAAGGCCCAT GGCCATGGGG CTTCAAGGAA AGAAACCTCA
    CAGTTTTTCC CATCTAGTCC ACCACCCCAT TCCCCATTAA GTCATGGGCA TATCCCCAGT
    GCCATTGTTC TTCCTAATGC TACTCATGAC TACAACACGT CTTTCTCAAA CTCCAATGCT
    CACAAAGCTG AAAAGAAGCT TCAAAACATT GACCACCCAC TTACAAAGTC ATCCAGTAAA
    AGAGATCACC GGCGTTCTGT GGATTCCAGA AATACCCTCA ATGATCTCCT GAAGCATTTA
    AATGACCCAA ACAGCAACCC CAAAGCCATC ATGGGAGATA TCCAGATGGC CCACCAGACC
    CTAATGCTGG ATCCTGTGGG ACCTATGTCT GAGGTCCCGC CCAAGGTCCC TAACCGGGAG
    GCATCCCTAT ACTCTCCTCC TTCAACTCTC CCCAGAAATA GCCCAACCAA GCGAGTGGAT
    GTCCCCACCA CTCCTGGGGT CCCAATGACT TCTCTGGAAA GACAAAGGGG TTACCACAAA
    AATTCCTCCC AGAGGCACTC TATATCTGCT ATGCCTAAAA ACTTAAACTC ACCAAATGGT
    GTTTTGTTAT CCAGACAGCC TAGCATGAAC CGTGGTGGGT ATGTGCCCAC CTCCACTGGG
    GCAAAGGTGG ACTATATTCA GGGAGCACCA GTGAGTGTTC ATCTGCAGCC TTCCCTCTCC
    AGACAGAGCA GCTATACCAG TAATGGCACC CTTCCTAGGA CGGGACTAAA GAGGACACCG
    TCCTTAAAAC CTGATGTGCC ACCAAAGCCT TCATTTGTTC CTCAAACCAC ATCTGTCAGA
    CCACTGAACA AATACACTTA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe28816
    Clone ID Related Accession (Same CDS sequence) XM_005638384.2 , XM_005638384.3
    Accession Version XM_005638384.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3165bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product semaphorin-6D isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005638384.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGGTTCT TCCTGCTTTG TGCCTACATG CTGCTCCTGA TGATTTCCCA GCTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTC AATACTGTTG ATTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AATGAATCCC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAGTCCCCAA GACAGAAGTA ATACCAAGCA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ACCGAGAAAA CTGTGCTATG AAGGGCAAAC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTCCC AAGAAATGAT GAGATGGTTT TTGTATGTGG TACCAATGCA
    TTCAATCCCA TGTGCAGATA CTATAGGTTG AATACCTTAG AGTATGATGG AGAGGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTAT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC CCTTCGTACA ATAAAATATG ATTCCAAATG GATAAAAGAG
    CCACATTTTC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAATAATTT AGGCAAGGCT GTATATTCCC GTGTGGCCCG CATATGTAAA
    AATGACATGG GTGGCTCGCA GCGGGTCCTG GAGAAACACT GGACTTCATT TCTGAAGGCT
    CGGCTTAACT GCTCTGTCCC CGGAGATTCG TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACGGACATCA TACAAATCAA TGGCATCCCC ACTGTGGTCG GGGTGTTTAC CACACAGCTC
    AACAGCATTC CTGGGTCTGC AGTGTGTGCA TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGCT TTAAAGAACA GAAAACCCCA GATTCTGTTT GGACAGCAGT CCCTGAAGAC
    AAAGTACCCA AGCCAAGGCC TGGCTGTTGT GCAAAGCATG GCCTTGCCGA AGCTTATAAA
    ACCTCCATAG ATTTCCCTGA CGAAACCCTG TCATTCATCA AATCCCATCC GCTAATGGAC
    GCTGCTGTAC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCTGTTGA CCACACTGCT GGGCCCCATC AGAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTGCTTAAA GTTTTGGCAA AGACCAGCCC TTTCTCTTTG
    AATGACAGTG TATTACTGGA AGAGATTGAA GCATACAACC AAGCAAAGTG CAATGCTGAG
    AATGAGGAAG ACAGAAGGGT CATCTCCTTA CAGTTGGATA AAGACCATCA CGCTGTGTAC
    GTGGCATTCT CTAGCTGCGT CATCCGCCTC CCTCTCAGCC GTTGCGAGCG TTACGGATCC
    TGTAAGAAGT CTTGTGTTGC ATCTCGGGAC CCATACTGTG GCTGGTTAAG CCAGGGGGCC
    TGTGGCAGAG TGACCCCAGG GATGCTCGCT GGAGGATATG AACAAGACAC AGAGTACGGC
    AACACGGCCC ACCTAGGGGA CTGCCATGAC ATGGAGGTAT CTTCATCATC TGTTACCACA
    ATGGCAAGTA TCCCAGAAAT TACACCTAAA GTGATTGATA CCTGGAGGCC TAAACTGACG
    AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAACACTT CTGATTTTAC TGATCCTTTA
    TCGGGTATCC CAAAGGGTGT GCGATGGGAA GTCCAATCGG GAGAGTCCAA CCAGATGGTC
    CACATGAACG TCCTCATCAC CTGTGTCTTT GCCGCTTTTG TTTTGGGTGC GTTCATTGCA
    GGGGTGGCAG TATACTGCTA TCGTGACATG TTTGTTCGGA AAAACAGAAA GATTCATAAG
    GATGCAGAAT CTGCCCAGTC GTGCACAGAC TCCAGTGGAA GTTTTGCCAA ACTGAATGGT
    CTGTTTGATA GCCCGGTCAA GGAATATCAA CAGAATATCG ATTCTCCCAA ATTATACAGT
    AACCTGCTGA CTAGTCGGAA AGAGCTGCCA CCCAATGGAG ATACCAAATC CATGGTGATG
    GACCATCGAG GCCAACCTCC CGAACTGGCT GCTCTCCCCA CGCCTGAGTC AACTCCTGTG
    CTTCACCAGA AGACCCTGCA GGCCATGAAG AGCCACTCAG ATAAGGCCCA TGGCCATGGG
    GCTTCAAGGA AAGAAACCTC ACAGTTTTTC CCATCTAGTC CACCACCCCA TTCCCCATTA
    AGTCATGGGC ATATCCCCAG TGCCATTGTT CTTCCTAATG CTACTCATGA CTACAACACG
    TCTTTCTCAA ACTCCAATGC TCACAAAGCT GAAAAGAAGC TTCAAAACAT TGACCACCCA
    CTTACAAAGT CATCCAGTAA AAGAGATCAC CGGCGTTCTG TGGATTCCAG AAATACCCTC
    AATGATCTCC TGAAGCATTT AAATGACCCA AACAGCAACC CCAAAGCCAT CATGGGAGAT
    ATCCAGATGG CCCACCAGAC CCTAATGCTG GATCCTGTGG GACCTATGTC TGAGGTCCCG
    CCCAAGGTCC CTAACCGGGA GGCATCCCTA TACTCTCCTC CTTCAACTCT CCCCAGAAAT
    AGCCCAACCA AGCGAGTGGA TGTCCCCACC ACTCCTGGGG TCCCAATGAC TTCTCTGGAA
    AGACAAAGGG GTTACCACAA AAATTCCTCC CAGAGGCACT CTATATCTGC TATGCCTAAA
    AACTTAAACT CACCAAATGG TGTTTTGTTA TCCAGACAGC CTAGCATGAA CCGTGGTGGG
    TATGTGCCCA CCTCCACTGG GGCAAAGGTG GACTATATTC AGGGAGCACC AGTGAGTGTT
    CATCTGCAGC CTTCCCTCTC CAGACAGAGC AGCTATACCA GTAATGGCAC CCTTCCTAGG
    ACGGGACTAA AGAGGACACC GTCCTTAAAA CCTGATGTGC CACCAAAGCC TTCATTTGTT
    CCTCAAACCA CATCTGTCAG ACCACTGAAC AAATACACTT ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005638441.1
    CDS314..3478
    Translation

    Target ORF information:

    RefSeq Version XM_005638384.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (SEMA6D), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005638384.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGGTTCT TCCTGCTTTG TGCCTACATG CTGCTCCTGA TGATTTCCCA GCTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTC AATACTGTTG ATTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AATGAATCCC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAGTCCCCAA GACAGAAGTA ATACCAAGCA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ACCGAGAAAA CTGTGCTATG AAGGGCAAAC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTCCC AAGAAATGAT GAGATGGTTT TTGTATGTGG TACCAATGCA
    TTCAATCCCA TGTGCAGATA CTATAGGTTG AATACCTTAG AGTATGATGG AGAGGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTAT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC CCTTCGTACA ATAAAATATG ATTCCAAATG GATAAAAGAG
    CCACATTTTC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAATAATTT AGGCAAGGCT GTATATTCCC GTGTGGCCCG CATATGTAAA
    AATGACATGG GTGGCTCGCA GCGGGTCCTG GAGAAACACT GGACTTCATT TCTGAAGGCT
    CGGCTTAACT GCTCTGTCCC CGGAGATTCG TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACGGACATCA TACAAATCAA TGGCATCCCC ACTGTGGTCG GGGTGTTTAC CACACAGCTC
    AACAGCATTC CTGGGTCTGC AGTGTGTGCA TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGCT TTAAAGAACA GAAAACCCCA GATTCTGTTT GGACAGCAGT CCCTGAAGAC
    AAAGTACCCA AGCCAAGGCC TGGCTGTTGT GCAAAGCATG GCCTTGCCGA AGCTTATAAA
    ACCTCCATAG ATTTCCCTGA CGAAACCCTG TCATTCATCA AATCCCATCC GCTAATGGAC
    GCTGCTGTAC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCTGTTGA CCACACTGCT GGGCCCCATC AGAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTGCTTAAA GTTTTGGCAA AGACCAGCCC TTTCTCTTTG
    AATGACAGTG TATTACTGGA AGAGATTGAA GCATACAACC AAGCAAAGTG CAATGCTGAG
    AATGAGGAAG ACAGAAGGGT CATCTCCTTA CAGTTGGATA AAGACCATCA CGCTGTGTAC
    GTGGCATTCT CTAGCTGCGT CATCCGCCTC CCTCTCAGCC GTTGCGAGCG TTACGGATCC
    TGTAAGAAGT CTTGTGTTGC ATCTCGGGAC CCATACTGTG GCTGGTTAAG CCAGGGGGCC
    TGTGGCAGAG TGACCCCAGG GATGCTCGCT GGAGGATATG AACAAGACAC AGAGTACGGC
    AACACGGCCC ACCTAGGGGA CTGCCATGAC ATGGAGGTAT CTTCATCATC TGTTACCACA
    ATGGCAAGTA TCCCAGAAAT TACACCTAAA GTGATTGATA CCTGGAGGCC TAAACTGACG
    AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAACACTT CTGATTTTAC TGATCCTTTA
    TCGGGTATCC CAAAGGGTGT GCGATGGGAA GTCCAATCGG GAGAGTCCAA CCAGATGGTC
    CACATGAACG TCCTCATCAC CTGTGTCTTT GCCGCTTTTG TTTTGGGTGC GTTCATTGCA
    GGGGTGGCAG TATACTGCTA TCGTGACATG TTTGTTCGGA AAAACAGAAA GATTCATAAG
    GATGCAGAAT CTGCCCAGTC GTGCACAGAC TCCAGTGGAA GTTTTGCCAA ACTGAATGGT
    CTGTTTGATA GCCCGGTCAA GGAATATCAA CAGAATATCG ATTCTCCCAA ATTATACAGT
    AACCTGCTGA CTAGTCGGAA AGAGCTGCCA CCCAATGGAG ATACCAAATC CATGGTGATG
    GACCATCGAG GCCAACCTCC CGAACTGGCT GCTCTCCCCA CGCCTGAGTC AACTCCTGTG
    CTTCACCAGA AGACCCTGCA GGCCATGAAG AGCCACTCAG ATAAGGCCCA TGGCCATGGG
    GCTTCAAGGA AAGAAACCTC ACAGTTTTTC CCATCTAGTC CACCACCCCA TTCCCCATTA
    AGTCATGGGC ATATCCCCAG TGCCATTGTT CTTCCTAATG CTACTCATGA CTACAACACG
    TCTTTCTCAA ACTCCAATGC TCACAAAGCT GAAAAGAAGC TTCAAAACAT TGACCACCCA
    CTTACAAAGT CATCCAGTAA AAGAGATCAC CGGCGTTCTG TGGATTCCAG AAATACCCTC
    AATGATCTCC TGAAGCATTT AAATGACCCA AACAGCAACC CCAAAGCCAT CATGGGAGAT
    ATCCAGATGG CCCACCAGAC CCTAATGCTG GATCCTGTGG GACCTATGTC TGAGGTCCCG
    CCCAAGGTCC CTAACCGGGA GGCATCCCTA TACTCTCCTC CTTCAACTCT CCCCAGAAAT
    AGCCCAACCA AGCGAGTGGA TGTCCCCACC ACTCCTGGGG TCCCAATGAC TTCTCTGGAA
    AGACAAAGGG GTTACCACAA AAATTCCTCC CAGAGGCACT CTATATCTGC TATGCCTAAA
    AACTTAAACT CACCAAATGG TGTTTTGTTA TCCAGACAGC CTAGCATGAA CCGTGGTGGG
    TATGTGCCCA CCTCCACTGG GGCAAAGGTG GACTATATTC AGGGAGCACC AGTGAGTGTT
    CATCTGCAGC CTTCCCTCTC CAGACAGAGC AGCTATACCA GTAATGGCAC CCTTCCTAGG
    ACGGGACTAA AGAGGACACC GTCCTTAAAA CCTGATGTGC CACCAAAGCC TTCATTTGTT
    CCTCAAACCA CATCTGTCAG ACCACTGAAC AAATACACTT ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe28816
    Clone ID Related Accession (Same CDS sequence) XM_005638384.2 , XM_005638384.3
    Accession Version XM_005638384.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3165bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product semaphorin-6D isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005638384.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGGTTCT TCCTGCTTTG TGCCTACATG CTGCTCCTGA TGATTTCCCA GCTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTC AATACTGTTG ATTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AATGAATCCC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAGTCCCCAA GACAGAAGTA ATACCAAGCA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ACCGAGAAAA CTGTGCTATG AAGGGCAAAC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTCCC AAGAAATGAT GAGATGGTTT TTGTATGTGG TACCAATGCA
    TTCAATCCCA TGTGCAGATA CTATAGGTTG AATACCTTAG AGTATGATGG AGAGGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTAT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC CCTTCGTACA ATAAAATATG ATTCCAAATG GATAAAAGAG
    CCACATTTTC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAATAATTT AGGCAAGGCT GTATATTCCC GTGTGGCCCG CATATGTAAA
    AATGACATGG GTGGCTCGCA GCGGGTCCTG GAGAAACACT GGACTTCATT TCTGAAGGCT
    CGGCTTAACT GCTCTGTCCC CGGAGATTCG TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACGGACATCA TACAAATCAA TGGCATCCCC ACTGTGGTCG GGGTGTTTAC CACACAGCTC
    AACAGCATTC CTGGGTCTGC AGTGTGTGCA TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGCT TTAAAGAACA GAAAACCCCA GATTCTGTTT GGACAGCAGT CCCTGAAGAC
    AAAGTACCCA AGCCAAGGCC TGGCTGTTGT GCAAAGCATG GCCTTGCCGA AGCTTATAAA
    ACCTCCATAG ATTTCCCTGA CGAAACCCTG TCATTCATCA AATCCCATCC GCTAATGGAC
    GCTGCTGTAC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCTGTTGA CCACACTGCT GGGCCCCATC AGAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTGCTTAAA GTTTTGGCAA AGACCAGCCC TTTCTCTTTG
    AATGACAGTG TATTACTGGA AGAGATTGAA GCATACAACC AAGCAAAGTG CAATGCTGAG
    AATGAGGAAG ACAGAAGGGT CATCTCCTTA CAGTTGGATA AAGACCATCA CGCTGTGTAC
    GTGGCATTCT CTAGCTGCGT CATCCGCCTC CCTCTCAGCC GTTGCGAGCG TTACGGATCC
    TGTAAGAAGT CTTGTGTTGC ATCTCGGGAC CCATACTGTG GCTGGTTAAG CCAGGGGGCC
    TGTGGCAGAG TGACCCCAGG GATGCTCGCT GGAGGATATG AACAAGACAC AGAGTACGGC
    AACACGGCCC ACCTAGGGGA CTGCCATGAC ATGGAGGTAT CTTCATCATC TGTTACCACA
    ATGGCAAGTA TCCCAGAAAT TACACCTAAA GTGATTGATA CCTGGAGGCC TAAACTGACG
    AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAACACTT CTGATTTTAC TGATCCTTTA
    TCGGGTATCC CAAAGGGTGT GCGATGGGAA GTCCAATCGG GAGAGTCCAA CCAGATGGTC
    CACATGAACG TCCTCATCAC CTGTGTCTTT GCCGCTTTTG TTTTGGGTGC GTTCATTGCA
    GGGGTGGCAG TATACTGCTA TCGTGACATG TTTGTTCGGA AAAACAGAAA GATTCATAAG
    GATGCAGAAT CTGCCCAGTC GTGCACAGAC TCCAGTGGAA GTTTTGCCAA ACTGAATGGT
    CTGTTTGATA GCCCGGTCAA GGAATATCAA CAGAATATCG ATTCTCCCAA ATTATACAGT
    AACCTGCTGA CTAGTCGGAA AGAGCTGCCA CCCAATGGAG ATACCAAATC CATGGTGATG
    GACCATCGAG GCCAACCTCC CGAACTGGCT GCTCTCCCCA CGCCTGAGTC AACTCCTGTG
    CTTCACCAGA AGACCCTGCA GGCCATGAAG AGCCACTCAG ATAAGGCCCA TGGCCATGGG
    GCTTCAAGGA AAGAAACCTC ACAGTTTTTC CCATCTAGTC CACCACCCCA TTCCCCATTA
    AGTCATGGGC ATATCCCCAG TGCCATTGTT CTTCCTAATG CTACTCATGA CTACAACACG
    TCTTTCTCAA ACTCCAATGC TCACAAAGCT GAAAAGAAGC TTCAAAACAT TGACCACCCA
    CTTACAAAGT CATCCAGTAA AAGAGATCAC CGGCGTTCTG TGGATTCCAG AAATACCCTC
    AATGATCTCC TGAAGCATTT AAATGACCCA AACAGCAACC CCAAAGCCAT CATGGGAGAT
    ATCCAGATGG CCCACCAGAC CCTAATGCTG GATCCTGTGG GACCTATGTC TGAGGTCCCG
    CCCAAGGTCC CTAACCGGGA GGCATCCCTA TACTCTCCTC CTTCAACTCT CCCCAGAAAT
    AGCCCAACCA AGCGAGTGGA TGTCCCCACC ACTCCTGGGG TCCCAATGAC TTCTCTGGAA
    AGACAAAGGG GTTACCACAA AAATTCCTCC CAGAGGCACT CTATATCTGC TATGCCTAAA
    AACTTAAACT CACCAAATGG TGTTTTGTTA TCCAGACAGC CTAGCATGAA CCGTGGTGGG
    TATGTGCCCA CCTCCACTGG GGCAAAGGTG GACTATATTC AGGGAGCACC AGTGAGTGTT
    CATCTGCAGC CTTCCCTCTC CAGACAGAGC AGCTATACCA GTAATGGCAC CCTTCCTAGG
    ACGGGACTAA AGAGGACACC GTCCTTAAAA CCTGATGTGC CACCAAAGCC TTCATTTGTT
    CCTCAAACCA CATCTGTCAG ACCACTGAAC AAATACACTT ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005638441.1
    CDS287..3451
    Translation

    Target ORF information:

    RefSeq Version XM_005638384.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris semaphorin 6D (SEMA6D), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005638384.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGGTTCT TCCTGCTTTG TGCCTACATG CTGCTCCTGA TGATTTCCCA GCTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTC AATACTGTTG ATTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AATGAATCCC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAGTCCCCAA GACAGAAGTA ATACCAAGCA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ACCGAGAAAA CTGTGCTATG AAGGGCAAAC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTCCC AAGAAATGAT GAGATGGTTT TTGTATGTGG TACCAATGCA
    TTCAATCCCA TGTGCAGATA CTATAGGTTG AATACCTTAG AGTATGATGG AGAGGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTAT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC CCTTCGTACA ATAAAATATG ATTCCAAATG GATAAAAGAG
    CCACATTTTC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAATAATTT AGGCAAGGCT GTATATTCCC GTGTGGCCCG CATATGTAAA
    AATGACATGG GTGGCTCGCA GCGGGTCCTG GAGAAACACT GGACTTCATT TCTGAAGGCT
    CGGCTTAACT GCTCTGTCCC CGGAGATTCG TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACGGACATCA TACAAATCAA TGGCATCCCC ACTGTGGTCG GGGTGTTTAC CACACAGCTC
    AACAGCATTC CTGGGTCTGC AGTGTGTGCA TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGCT TTAAAGAACA GAAAACCCCA GATTCTGTTT GGACAGCAGT CCCTGAAGAC
    AAAGTACCCA AGCCAAGGCC TGGCTGTTGT GCAAAGCATG GCCTTGCCGA AGCTTATAAA
    ACCTCCATAG ATTTCCCTGA CGAAACCCTG TCATTCATCA AATCCCATCC GCTAATGGAC
    GCTGCTGTAC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCTGTTGA CCACACTGCT GGGCCCCATC AGAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTGCTTAAA GTTTTGGCAA AGACCAGCCC TTTCTCTTTG
    AATGACAGTG TATTACTGGA AGAGATTGAA GCATACAACC AAGCAAAGTG CAATGCTGAG
    AATGAGGAAG ACAGAAGGGT CATCTCCTTA CAGTTGGATA AAGACCATCA CGCTGTGTAC
    GTGGCATTCT CTAGCTGCGT CATCCGCCTC CCTCTCAGCC GTTGCGAGCG TTACGGATCC
    TGTAAGAAGT CTTGTGTTGC ATCTCGGGAC CCATACTGTG GCTGGTTAAG CCAGGGGGCC
    TGTGGCAGAG TGACCCCAGG GATGCTCGCT GGAGGATATG AACAAGACAC AGAGTACGGC
    AACACGGCCC ACCTAGGGGA CTGCCATGAC ATGGAGGTAT CTTCATCATC TGTTACCACA
    ATGGCAAGTA TCCCAGAAAT TACACCTAAA GTGATTGATA CCTGGAGGCC TAAACTGACG
    AGCTCCCGGA AATTTGTAGT TCAAGATGAC CCAAACACTT CTGATTTTAC TGATCCTTTA
    TCGGGTATCC CAAAGGGTGT GCGATGGGAA GTCCAATCGG GAGAGTCCAA CCAGATGGTC
    CACATGAACG TCCTCATCAC CTGTGTCTTT GCCGCTTTTG TTTTGGGTGC GTTCATTGCA
    GGGGTGGCAG TATACTGCTA TCGTGACATG TTTGTTCGGA AAAACAGAAA GATTCATAAG
    GATGCAGAAT CTGCCCAGTC GTGCACAGAC TCCAGTGGAA GTTTTGCCAA ACTGAATGGT
    CTGTTTGATA GCCCGGTCAA GGAATATCAA CAGAATATCG ATTCTCCCAA ATTATACAGT
    AACCTGCTGA CTAGTCGGAA AGAGCTGCCA CCCAATGGAG ATACCAAATC CATGGTGATG
    GACCATCGAG GCCAACCTCC CGAACTGGCT GCTCTCCCCA CGCCTGAGTC AACTCCTGTG
    CTTCACCAGA AGACCCTGCA GGCCATGAAG AGCCACTCAG ATAAGGCCCA TGGCCATGGG
    GCTTCAAGGA AAGAAACCTC ACAGTTTTTC CCATCTAGTC CACCACCCCA TTCCCCATTA
    AGTCATGGGC ATATCCCCAG TGCCATTGTT CTTCCTAATG CTACTCATGA CTACAACACG
    TCTTTCTCAA ACTCCAATGC TCACAAAGCT GAAAAGAAGC TTCAAAACAT TGACCACCCA
    CTTACAAAGT CATCCAGTAA AAGAGATCAC CGGCGTTCTG TGGATTCCAG AAATACCCTC
    AATGATCTCC TGAAGCATTT AAATGACCCA AACAGCAACC CCAAAGCCAT CATGGGAGAT
    ATCCAGATGG CCCACCAGAC CCTAATGCTG GATCCTGTGG GACCTATGTC TGAGGTCCCG
    CCCAAGGTCC CTAACCGGGA GGCATCCCTA TACTCTCCTC CTTCAACTCT CCCCAGAAAT
    AGCCCAACCA AGCGAGTGGA TGTCCCCACC ACTCCTGGGG TCCCAATGAC TTCTCTGGAA
    AGACAAAGGG GTTACCACAA AAATTCCTCC CAGAGGCACT CTATATCTGC TATGCCTAAA
    AACTTAAACT CACCAAATGG TGTTTTGTTA TCCAGACAGC CTAGCATGAA CCGTGGTGGG
    TATGTGCCCA CCTCCACTGG GGCAAAGGTG GACTATATTC AGGGAGCACC AGTGAGTGTT
    CATCTGCAGC CTTCCCTCTC CAGACAGAGC AGCTATACCA GTAATGGCAC CCTTCCTAGG
    ACGGGACTAA AGAGGACACC GTCCTTAAAA CCTGATGTGC CACCAAAGCC TTCATTTGTT
    CCTCAAACCA CATCTGTCAG ACCACTGAAC AAATACACTT ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe28817
    Clone ID Related Accession (Same CDS sequence) XM_005638385.2 , XM_005638385.3
    Accession Version XM_005638385.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3093bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product semaphorin-6D isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005638385.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAGGTTCT TCCTGCTTTG TGCCTACATG CTGCTCCTGA TGATTTCCCA GCTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTC AATACTGTTG ATTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AATGAATCCC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAGTCCCCAA GACAGAAGTA ATACCAAGCA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ACCGAGAAAA CTGTGCTATG AAGGGCAAAC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTCCC AAGAAATGAT GAGATGGTTT TTGTATGTGG TACCAATGCA
    TTCAATCCCA TGTGCAGATA CTATAGGTTG AATACCTTAG AGTATGATGG AGAGGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTAT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC CCTTCGTACA ATAAAATATG ATTCCAAATG GATAAAAGAG
    CCACATTTTC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAATAATTT AGGCAAGGCT GTATATTCCC GTGTGGCCCG CATATGTAAA
    AATGACATGG GTGGCTCGCA GCGGGTCCTG GAGAAACACT GGACTTCATT TCTGAAGGCT
    CGGCTTAACT GCTCTGTCCC CGGAGATTCG TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACGGACATCA TACAAATCAA TGGCATCCCC ACTGTGGTCG GGGTGTTTAC CACACAGCTC
    AACAGCATTC CTGGGTCTGC AGTGTGTGCA TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGCT TTAAAGAACA GAAAACCCCA GATTCTGTTT GGACAGCAGT CCCTGAAGAC
    AAAGTACCCA AGCCAAGGCC TGGCTGTTGT GCAAAGCATG GCCTTGCCGA AGCTTATAAA
    ACCTCCATAG ATTTCCCTGA CGAAACCCTG TCATTCATCA AATCCCATCC GCTAATGGAC
    GCTGCTGTAC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCTGTTGA CCACACTGCT GGGCCCCATC AGAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTGCTTAAA GTTTTGGCAA AGACCAGCCC TTTCTCTTTG
    AATGACAGTG TATTACTGGA AGAGATTGAA GCATACAACC AAGCAAAGTG CAATGCTGAG
    AATGAGGAAG ACAGAAGGGT CATCTCCTTA CAGTTGGATA AAGACCATCA CGCTGTGTAC
    GTGGCATTCT CTAGCTGCGT CATCCGCCTC CCTCTCAGCC GTTGCGAGCG TTACGGATCC
    TGTAAGAAGT CTTGTGTTGC ATCTCGGGAC CCATACTGTG GCTGGTTAAG CCAGGGGGCC
    TGTGGCAGAG TGACCCCAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATATGA ACAAGACACA GAGTACGGCA ACACGGCCCA CCTAGGGGAC
    TGCCATGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGGTGTGC GATGGGAAGT CCAATCGGGA GAGTCCAACC AGATGGTCCA CATGAACGTC
    CTCATCACCT GTGTCTTTGC CGCTTTTGTT TTGGGTGCGT TCATTGCAGG GGTGGCAGTA
    TACTGCTATC GTGACATGTT TGTTCGGAAA AACAGAAAGA TTCATAAGGA TGCAGAATCT
    GCCCAGTCGT GCACAGACTC CAGTGGAAGT TTTGCCAAAC TGAATGGTCT GTTTGATAGC
    CCGGTCAAGG AATATCAACA GAATATCGAT TCTCCCAAAT TATACAGTAA CCTGCTGACT
    AGTCGGAAAG AGCTGCCACC CAATGGAGAT ACCAAATCCA TGGTGATGGA CCATCGAGGC
    CAACCTCCCG AACTGGCTGC TCTCCCCACG CCTGAGTCAA CTCCTGTGCT TCACCAGAAG
    ACCCTGCAGG CCATGAAGAG CCACTCAGAT AAGGCCCATG GCCATGGGGC TTCAAGGAAA
    GAAACCTCAC AGTTTTTCCC ATCTAGTCCA CCACCCCATT CCCCATTAAG TCATGGGCAT
    ATCCCCAGTG CCATTGTTCT TCCTAATGCT ACTCATGACT ACAACACGTC TTTCTCAAAC
    TCCAATGCTC ACAAAGCTGA AAAGAAGCTT CAAAACATTG ACCACCCACT TACAAAGTCA
    TCCAGTAAAA GAGATCACCG GCGTTCTGTG GATTCCAGAA ATACCCTCAA TGATCTCCTG
    AAGCATTTAA ATGACCCAAA CAGCAACCCC AAAGCCATCA TGGGAGATAT CCAGATGGCC
    CACCAGACCC TAATGCTGGA TCCTGTGGGA CCTATGTCTG AGGTCCCGCC CAAGGTCCCT
    AACCGGGAGG CATCCCTATA CTCTCCTCCT TCAACTCTCC CCAGAAATAG CCCAACCAAG
    CGAGTGGATG TCCCCACCAC TCCTGGGGTC CCAATGACTT CTCTGGAAAG ACAAAGGGGT
    TACCACAAAA ATTCCTCCCA GAGGCACTCT ATATCTGCTA TGCCTAAAAA CTTAAACTCA
    CCAAATGGTG TTTTGTTATC CAGACAGCCT AGCATGAACC GTGGTGGGTA TGTGCCCACC
    TCCACTGGGG CAAAGGTGGA CTATATTCAG GGAGCACCAG TGAGTGTTCA TCTGCAGCCT
    TCCCTCTCCA GACAGAGCAG CTATACCAGT AATGGCACCC TTCCTAGGAC GGGACTAAAG
    AGGACACCGT CCTTAAAACC TGATGTGCCA CCAAAGCCTT CATTTGTTCC TCAAACCACA
    TCTGTCAGAC CACTGAACAA ATACACTTAC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005638442.1
    CDS314..3406
    Translation

    Target ORF information:

    RefSeq Version XM_005638385.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (SEMA6D), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005638385.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAGGTTCT TCCTGCTTTG TGCCTACATG CTGCTCCTGA TGATTTCCCA GCTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTC AATACTGTTG ATTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AATGAATCCC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAGTCCCCAA GACAGAAGTA ATACCAAGCA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ACCGAGAAAA CTGTGCTATG AAGGGCAAAC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTCCC AAGAAATGAT GAGATGGTTT TTGTATGTGG TACCAATGCA
    TTCAATCCCA TGTGCAGATA CTATAGGTTG AATACCTTAG AGTATGATGG AGAGGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTAT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC CCTTCGTACA ATAAAATATG ATTCCAAATG GATAAAAGAG
    CCACATTTTC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAATAATTT AGGCAAGGCT GTATATTCCC GTGTGGCCCG CATATGTAAA
    AATGACATGG GTGGCTCGCA GCGGGTCCTG GAGAAACACT GGACTTCATT TCTGAAGGCT
    CGGCTTAACT GCTCTGTCCC CGGAGATTCG TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACGGACATCA TACAAATCAA TGGCATCCCC ACTGTGGTCG GGGTGTTTAC CACACAGCTC
    AACAGCATTC CTGGGTCTGC AGTGTGTGCA TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGCT TTAAAGAACA GAAAACCCCA GATTCTGTTT GGACAGCAGT CCCTGAAGAC
    AAAGTACCCA AGCCAAGGCC TGGCTGTTGT GCAAAGCATG GCCTTGCCGA AGCTTATAAA
    ACCTCCATAG ATTTCCCTGA CGAAACCCTG TCATTCATCA AATCCCATCC GCTAATGGAC
    GCTGCTGTAC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCTGTTGA CCACACTGCT GGGCCCCATC AGAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTGCTTAAA GTTTTGGCAA AGACCAGCCC TTTCTCTTTG
    AATGACAGTG TATTACTGGA AGAGATTGAA GCATACAACC AAGCAAAGTG CAATGCTGAG
    AATGAGGAAG ACAGAAGGGT CATCTCCTTA CAGTTGGATA AAGACCATCA CGCTGTGTAC
    GTGGCATTCT CTAGCTGCGT CATCCGCCTC CCTCTCAGCC GTTGCGAGCG TTACGGATCC
    TGTAAGAAGT CTTGTGTTGC ATCTCGGGAC CCATACTGTG GCTGGTTAAG CCAGGGGGCC
    TGTGGCAGAG TGACCCCAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATATGA ACAAGACACA GAGTACGGCA ACACGGCCCA CCTAGGGGAC
    TGCCATGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGGTGTGC GATGGGAAGT CCAATCGGGA GAGTCCAACC AGATGGTCCA CATGAACGTC
    CTCATCACCT GTGTCTTTGC CGCTTTTGTT TTGGGTGCGT TCATTGCAGG GGTGGCAGTA
    TACTGCTATC GTGACATGTT TGTTCGGAAA AACAGAAAGA TTCATAAGGA TGCAGAATCT
    GCCCAGTCGT GCACAGACTC CAGTGGAAGT TTTGCCAAAC TGAATGGTCT GTTTGATAGC
    CCGGTCAAGG AATATCAACA GAATATCGAT TCTCCCAAAT TATACAGTAA CCTGCTGACT
    AGTCGGAAAG AGCTGCCACC CAATGGAGAT ACCAAATCCA TGGTGATGGA CCATCGAGGC
    CAACCTCCCG AACTGGCTGC TCTCCCCACG CCTGAGTCAA CTCCTGTGCT TCACCAGAAG
    ACCCTGCAGG CCATGAAGAG CCACTCAGAT AAGGCCCATG GCCATGGGGC TTCAAGGAAA
    GAAACCTCAC AGTTTTTCCC ATCTAGTCCA CCACCCCATT CCCCATTAAG TCATGGGCAT
    ATCCCCAGTG CCATTGTTCT TCCTAATGCT ACTCATGACT ACAACACGTC TTTCTCAAAC
    TCCAATGCTC ACAAAGCTGA AAAGAAGCTT CAAAACATTG ACCACCCACT TACAAAGTCA
    TCCAGTAAAA GAGATCACCG GCGTTCTGTG GATTCCAGAA ATACCCTCAA TGATCTCCTG
    AAGCATTTAA ATGACCCAAA CAGCAACCCC AAAGCCATCA TGGGAGATAT CCAGATGGCC
    CACCAGACCC TAATGCTGGA TCCTGTGGGA CCTATGTCTG AGGTCCCGCC CAAGGTCCCT
    AACCGGGAGG CATCCCTATA CTCTCCTCCT TCAACTCTCC CCAGAAATAG CCCAACCAAG
    CGAGTGGATG TCCCCACCAC TCCTGGGGTC CCAATGACTT CTCTGGAAAG ACAAAGGGGT
    TACCACAAAA ATTCCTCCCA GAGGCACTCT ATATCTGCTA TGCCTAAAAA CTTAAACTCA
    CCAAATGGTG TTTTGTTATC CAGACAGCCT AGCATGAACC GTGGTGGGTA TGTGCCCACC
    TCCACTGGGG CAAAGGTGGA CTATATTCAG GGAGCACCAG TGAGTGTTCA TCTGCAGCCT
    TCCCTCTCCA GACAGAGCAG CTATACCAGT AATGGCACCC TTCCTAGGAC GGGACTAAAG
    AGGACACCGT CCTTAAAACC TGATGTGCCA CCAAAGCCTT CATTTGTTCC TCAAACCACA
    TCTGTCAGAC CACTGAACAA ATACACTTAC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe28817
    Clone ID Related Accession (Same CDS sequence) XM_005638385.2 , XM_005638385.3
    Accession Version XM_005638385.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3093bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product semaphorin-6D isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005638385.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAGGTTCT TCCTGCTTTG TGCCTACATG CTGCTCCTGA TGATTTCCCA GCTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTC AATACTGTTG ATTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AATGAATCCC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAGTCCCCAA GACAGAAGTA ATACCAAGCA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ACCGAGAAAA CTGTGCTATG AAGGGCAAAC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTCCC AAGAAATGAT GAGATGGTTT TTGTATGTGG TACCAATGCA
    TTCAATCCCA TGTGCAGATA CTATAGGTTG AATACCTTAG AGTATGATGG AGAGGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTAT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC CCTTCGTACA ATAAAATATG ATTCCAAATG GATAAAAGAG
    CCACATTTTC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAATAATTT AGGCAAGGCT GTATATTCCC GTGTGGCCCG CATATGTAAA
    AATGACATGG GTGGCTCGCA GCGGGTCCTG GAGAAACACT GGACTTCATT TCTGAAGGCT
    CGGCTTAACT GCTCTGTCCC CGGAGATTCG TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACGGACATCA TACAAATCAA TGGCATCCCC ACTGTGGTCG GGGTGTTTAC CACACAGCTC
    AACAGCATTC CTGGGTCTGC AGTGTGTGCA TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGCT TTAAAGAACA GAAAACCCCA GATTCTGTTT GGACAGCAGT CCCTGAAGAC
    AAAGTACCCA AGCCAAGGCC TGGCTGTTGT GCAAAGCATG GCCTTGCCGA AGCTTATAAA
    ACCTCCATAG ATTTCCCTGA CGAAACCCTG TCATTCATCA AATCCCATCC GCTAATGGAC
    GCTGCTGTAC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCTGTTGA CCACACTGCT GGGCCCCATC AGAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTGCTTAAA GTTTTGGCAA AGACCAGCCC TTTCTCTTTG
    AATGACAGTG TATTACTGGA AGAGATTGAA GCATACAACC AAGCAAAGTG CAATGCTGAG
    AATGAGGAAG ACAGAAGGGT CATCTCCTTA CAGTTGGATA AAGACCATCA CGCTGTGTAC
    GTGGCATTCT CTAGCTGCGT CATCCGCCTC CCTCTCAGCC GTTGCGAGCG TTACGGATCC
    TGTAAGAAGT CTTGTGTTGC ATCTCGGGAC CCATACTGTG GCTGGTTAAG CCAGGGGGCC
    TGTGGCAGAG TGACCCCAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATATGA ACAAGACACA GAGTACGGCA ACACGGCCCA CCTAGGGGAC
    TGCCATGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGGTGTGC GATGGGAAGT CCAATCGGGA GAGTCCAACC AGATGGTCCA CATGAACGTC
    CTCATCACCT GTGTCTTTGC CGCTTTTGTT TTGGGTGCGT TCATTGCAGG GGTGGCAGTA
    TACTGCTATC GTGACATGTT TGTTCGGAAA AACAGAAAGA TTCATAAGGA TGCAGAATCT
    GCCCAGTCGT GCACAGACTC CAGTGGAAGT TTTGCCAAAC TGAATGGTCT GTTTGATAGC
    CCGGTCAAGG AATATCAACA GAATATCGAT TCTCCCAAAT TATACAGTAA CCTGCTGACT
    AGTCGGAAAG AGCTGCCACC CAATGGAGAT ACCAAATCCA TGGTGATGGA CCATCGAGGC
    CAACCTCCCG AACTGGCTGC TCTCCCCACG CCTGAGTCAA CTCCTGTGCT TCACCAGAAG
    ACCCTGCAGG CCATGAAGAG CCACTCAGAT AAGGCCCATG GCCATGGGGC TTCAAGGAAA
    GAAACCTCAC AGTTTTTCCC ATCTAGTCCA CCACCCCATT CCCCATTAAG TCATGGGCAT
    ATCCCCAGTG CCATTGTTCT TCCTAATGCT ACTCATGACT ACAACACGTC TTTCTCAAAC
    TCCAATGCTC ACAAAGCTGA AAAGAAGCTT CAAAACATTG ACCACCCACT TACAAAGTCA
    TCCAGTAAAA GAGATCACCG GCGTTCTGTG GATTCCAGAA ATACCCTCAA TGATCTCCTG
    AAGCATTTAA ATGACCCAAA CAGCAACCCC AAAGCCATCA TGGGAGATAT CCAGATGGCC
    CACCAGACCC TAATGCTGGA TCCTGTGGGA CCTATGTCTG AGGTCCCGCC CAAGGTCCCT
    AACCGGGAGG CATCCCTATA CTCTCCTCCT TCAACTCTCC CCAGAAATAG CCCAACCAAG
    CGAGTGGATG TCCCCACCAC TCCTGGGGTC CCAATGACTT CTCTGGAAAG ACAAAGGGGT
    TACCACAAAA ATTCCTCCCA GAGGCACTCT ATATCTGCTA TGCCTAAAAA CTTAAACTCA
    CCAAATGGTG TTTTGTTATC CAGACAGCCT AGCATGAACC GTGGTGGGTA TGTGCCCACC
    TCCACTGGGG CAAAGGTGGA CTATATTCAG GGAGCACCAG TGAGTGTTCA TCTGCAGCCT
    TCCCTCTCCA GACAGAGCAG CTATACCAGT AATGGCACCC TTCCTAGGAC GGGACTAAAG
    AGGACACCGT CCTTAAAACC TGATGTGCCA CCAAAGCCTT CATTTGTTCC TCAAACCACA
    TCTGTCAGAC CACTGAACAA ATACACTTAC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005638442.1
    CDS288..3380
    Translation

    Target ORF information:

    RefSeq Version XM_005638385.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris semaphorin 6D (SEMA6D), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005638385.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAGGTTCT TCCTGCTTTG TGCCTACATG CTGCTCCTGA TGATTTCCCA GCTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTC AATACTGTTG ATTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AATGAATCCC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAGTCCCCAA GACAGAAGTA ATACCAAGCA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ACCGAGAAAA CTGTGCTATG AAGGGCAAAC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTCCC AAGAAATGAT GAGATGGTTT TTGTATGTGG TACCAATGCA
    TTCAATCCCA TGTGCAGATA CTATAGGTTG AATACCTTAG AGTATGATGG AGAGGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTAT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC CCTTCGTACA ATAAAATATG ATTCCAAATG GATAAAAGAG
    CCACATTTTC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAATAATTT AGGCAAGGCT GTATATTCCC GTGTGGCCCG CATATGTAAA
    AATGACATGG GTGGCTCGCA GCGGGTCCTG GAGAAACACT GGACTTCATT TCTGAAGGCT
    CGGCTTAACT GCTCTGTCCC CGGAGATTCG TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACGGACATCA TACAAATCAA TGGCATCCCC ACTGTGGTCG GGGTGTTTAC CACACAGCTC
    AACAGCATTC CTGGGTCTGC AGTGTGTGCA TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGCT TTAAAGAACA GAAAACCCCA GATTCTGTTT GGACAGCAGT CCCTGAAGAC
    AAAGTACCCA AGCCAAGGCC TGGCTGTTGT GCAAAGCATG GCCTTGCCGA AGCTTATAAA
    ACCTCCATAG ATTTCCCTGA CGAAACCCTG TCATTCATCA AATCCCATCC GCTAATGGAC
    GCTGCTGTAC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCTGTTGA CCACACTGCT GGGCCCCATC AGAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTGCTTAAA GTTTTGGCAA AGACCAGCCC TTTCTCTTTG
    AATGACAGTG TATTACTGGA AGAGATTGAA GCATACAACC AAGCAAAGTG CAATGCTGAG
    AATGAGGAAG ACAGAAGGGT CATCTCCTTA CAGTTGGATA AAGACCATCA CGCTGTGTAC
    GTGGCATTCT CTAGCTGCGT CATCCGCCTC CCTCTCAGCC GTTGCGAGCG TTACGGATCC
    TGTAAGAAGT CTTGTGTTGC ATCTCGGGAC CCATACTGTG GCTGGTTAAG CCAGGGGGCC
    TGTGGCAGAG TGACCCCAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATATGA ACAAGACACA GAGTACGGCA ACACGGCCCA CCTAGGGGAC
    TGCCATGAAA TTTTGCCTAC TTCAACTACA CCAGATTACA AAATATTTGG CGGTCCAACA
    TCTGGTGTGC GATGGGAAGT CCAATCGGGA GAGTCCAACC AGATGGTCCA CATGAACGTC
    CTCATCACCT GTGTCTTTGC CGCTTTTGTT TTGGGTGCGT TCATTGCAGG GGTGGCAGTA
    TACTGCTATC GTGACATGTT TGTTCGGAAA AACAGAAAGA TTCATAAGGA TGCAGAATCT
    GCCCAGTCGT GCACAGACTC CAGTGGAAGT TTTGCCAAAC TGAATGGTCT GTTTGATAGC
    CCGGTCAAGG AATATCAACA GAATATCGAT TCTCCCAAAT TATACAGTAA CCTGCTGACT
    AGTCGGAAAG AGCTGCCACC CAATGGAGAT ACCAAATCCA TGGTGATGGA CCATCGAGGC
    CAACCTCCCG AACTGGCTGC TCTCCCCACG CCTGAGTCAA CTCCTGTGCT TCACCAGAAG
    ACCCTGCAGG CCATGAAGAG CCACTCAGAT AAGGCCCATG GCCATGGGGC TTCAAGGAAA
    GAAACCTCAC AGTTTTTCCC ATCTAGTCCA CCACCCCATT CCCCATTAAG TCATGGGCAT
    ATCCCCAGTG CCATTGTTCT TCCTAATGCT ACTCATGACT ACAACACGTC TTTCTCAAAC
    TCCAATGCTC ACAAAGCTGA AAAGAAGCTT CAAAACATTG ACCACCCACT TACAAAGTCA
    TCCAGTAAAA GAGATCACCG GCGTTCTGTG GATTCCAGAA ATACCCTCAA TGATCTCCTG
    AAGCATTTAA ATGACCCAAA CAGCAACCCC AAAGCCATCA TGGGAGATAT CCAGATGGCC
    CACCAGACCC TAATGCTGGA TCCTGTGGGA CCTATGTCTG AGGTCCCGCC CAAGGTCCCT
    AACCGGGAGG CATCCCTATA CTCTCCTCCT TCAACTCTCC CCAGAAATAG CCCAACCAAG
    CGAGTGGATG TCCCCACCAC TCCTGGGGTC CCAATGACTT CTCTGGAAAG ACAAAGGGGT
    TACCACAAAA ATTCCTCCCA GAGGCACTCT ATATCTGCTA TGCCTAAAAA CTTAAACTCA
    CCAAATGGTG TTTTGTTATC CAGACAGCCT AGCATGAACC GTGGTGGGTA TGTGCCCACC
    TCCACTGGGG CAAAGGTGGA CTATATTCAG GGAGCACCAG TGAGTGTTCA TCTGCAGCCT
    TCCCTCTCCA GACAGAGCAG CTATACCAGT AATGGCACCC TTCCTAGGAC GGGACTAAAG
    AGGACACCGT CCTTAAAACC TGATGTGCCA CCAAAGCCTT CATTTGTTCC TCAAACCACA
    TCTGTCAGAC CACTGAACAA ATACACTTAC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe28818
    Clone ID Related Accession (Same CDS sequence) XM_003640053.3 , XM_003640053.4
    Accession Version XM_003640053.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3054bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product semaphorin-6D isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003640053.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGGTTCT TCCTGCTTTG TGCCTACATG CTGCTCCTGA TGATTTCCCA GCTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTC AATACTGTTG ATTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AATGAATCCC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAGTCCCCAA GACAGAAGTA ATACCAAGCA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ACCGAGAAAA CTGTGCTATG AAGGGCAAAC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTCCC AAGAAATGAT GAGATGGTTT TTGTATGTGG TACCAATGCA
    TTCAATCCCA TGTGCAGATA CTATAGGTTG AATACCTTAG AGTATGATGG AGAGGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTAT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC CCTTCGTACA ATAAAATATG ATTCCAAATG GATAAAAGAG
    CCACATTTTC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAATAATTT AGGCAAGGCT GTATATTCCC GTGTGGCCCG CATATGTAAA
    AATGACATGG GTGGCTCGCA GCGGGTCCTG GAGAAACACT GGACTTCATT TCTGAAGGCT
    CGGCTTAACT GCTCTGTCCC CGGAGATTCG TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACGGACATCA TACAAATCAA TGGCATCCCC ACTGTGGTCG GGGTGTTTAC CACACAGCTC
    AACAGCATTC CTGGGTCTGC AGTGTGTGCA TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGCT TTAAAGAACA GAAAACCCCA GATTCTGTTT GGACAGCAGT CCCTGAAGAC
    AAAGTACCCA AGCCAAGGCC TGGCTGTTGT GCAAAGCATG GCCTTGCCGA AGCTTATAAA
    ACCTCCATAG ATTTCCCTGA CGAAACCCTG TCATTCATCA AATCCCATCC GCTAATGGAC
    GCTGCTGTAC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCTGTTGA CCACACTGCT GGGCCCCATC AGAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTGCTTAAA GTTTTGGCAA AGACCAGCCC TTTCTCTTTG
    AATGACAGTG TATTACTGGA AGAGATTGAA GCATACAACC AAGCAAAGTG CAATGCTGAG
    AATGAGGAAG ACAGAAGGGT CATCTCCTTA CAGTTGGATA AAGACCATCA CGCTGTGTAC
    GTGGCATTCT CTAGCTGCGT CATCCGCCTC CCTCTCAGCC GTTGCGAGCG TTACGGATCC
    TGTAAGAAGT CTTGTGTTGC ATCTCGGGAC CCATACTGTG GCTGGTTAAG CCAGGGGGCC
    TGTGGCAGAG TGACCCCAGG GATGCTCGCT GGAGGATATG AACAAGACAC AGAGTACGGC
    AACACGGCCC ACCTAGGGGA CTGCCATGAA ATTTTGCCTA CTTCAACTAC ACCAGATTAC
    AAAATATTTG GCGGTCCAAC ATCTGGTGTG CGATGGGAAG TCCAATCGGG AGAGTCCAAC
    CAGATGGTCC ACATGAACGT CCTCATCACC TGTGTCTTTG CCGCTTTTGT TTTGGGTGCG
    TTCATTGCAG GGGTGGCAGT ATACTGCTAT CGTGACATGT TTGTTCGGAA AAACAGAAAG
    ATTCATAAGG ATGCAGAATC TGCCCAGTCG TGCACAGACT CCAGTGGAAG TTTTGCCAAA
    CTGAATGGTC TGTTTGATAG CCCGGTCAAG GAATATCAAC AGAATATCGA TTCTCCCAAA
    TTATACAGTA ACCTGCTGAC TAGTCGGAAA GAGCTGCCAC CCAATGGAGA TACCAAATCC
    ATGGTGATGG ACCATCGAGG CCAACCTCCC GAACTGGCTG CTCTCCCCAC GCCTGAGTCA
    ACTCCTGTGC TTCACCAGAA GACCCTGCAG GCCATGAAGA GCCACTCAGA TAAGGCCCAT
    GGCCATGGGG CTTCAAGGAA AGAAACCTCA CAGTTTTTCC CATCTAGTCC ACCACCCCAT
    TCCCCATTAA GTCATGGGCA TATCCCCAGT GCCATTGTTC TTCCTAATGC TACTCATGAC
    TACAACACGT CTTTCTCAAA CTCCAATGCT CACAAAGCTG AAAAGAAGCT TCAAAACATT
    GACCACCCAC TTACAAAGTC ATCCAGTAAA AGAGATCACC GGCGTTCTGT GGATTCCAGA
    AATACCCTCA ATGATCTCCT GAAGCATTTA AATGACCCAA ACAGCAACCC CAAAGCCATC
    ATGGGAGATA TCCAGATGGC CCACCAGACC CTAATGCTGG ATCCTGTGGG ACCTATGTCT
    GAGGTCCCGC CCAAGGTCCC TAACCGGGAG GCATCCCTAT ACTCTCCTCC TTCAACTCTC
    CCCAGAAATA GCCCAACCAA GCGAGTGGAT GTCCCCACCA CTCCTGGGGT CCCAATGACT
    TCTCTGGAAA GACAAAGGGG TTACCACAAA AATTCCTCCC AGAGGCACTC TATATCTGCT
    ATGCCTAAAA ACTTAAACTC ACCAAATGGT GTTTTGTTAT CCAGACAGCC TAGCATGAAC
    CGTGGTGGGT ATGTGCCCAC CTCCACTGGG GCAAAGGTGG ACTATATTCA GGGAGCACCA
    GTGAGTGTTC ATCTGCAGCC TTCCCTCTCC AGACAGAGCA GCTATACCAG TAATGGCACC
    CTTCCTAGGA CGGGACTAAA GAGGACACCG TCCTTAAAAC CTGATGTGCC ACCAAAGCCT
    TCATTTGTTC CTCAAACCAC ATCTGTCAGA CCACTGAACA AATACACTTA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003640101.1
    CDS314..3367
    Translation

    Target ORF information:

    RefSeq Version XM_003640053.3
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (SEMA6D), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003640053.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGGTTCT TCCTGCTTTG TGCCTACATG CTGCTCCTGA TGATTTCCCA GCTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTC AATACTGTTG ATTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AATGAATCCC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAGTCCCCAA GACAGAAGTA ATACCAAGCA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ACCGAGAAAA CTGTGCTATG AAGGGCAAAC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTCCC AAGAAATGAT GAGATGGTTT TTGTATGTGG TACCAATGCA
    TTCAATCCCA TGTGCAGATA CTATAGGTTG AATACCTTAG AGTATGATGG AGAGGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTAT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC CCTTCGTACA ATAAAATATG ATTCCAAATG GATAAAAGAG
    CCACATTTTC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAATAATTT AGGCAAGGCT GTATATTCCC GTGTGGCCCG CATATGTAAA
    AATGACATGG GTGGCTCGCA GCGGGTCCTG GAGAAACACT GGACTTCATT TCTGAAGGCT
    CGGCTTAACT GCTCTGTCCC CGGAGATTCG TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACGGACATCA TACAAATCAA TGGCATCCCC ACTGTGGTCG GGGTGTTTAC CACACAGCTC
    AACAGCATTC CTGGGTCTGC AGTGTGTGCA TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGCT TTAAAGAACA GAAAACCCCA GATTCTGTTT GGACAGCAGT CCCTGAAGAC
    AAAGTACCCA AGCCAAGGCC TGGCTGTTGT GCAAAGCATG GCCTTGCCGA AGCTTATAAA
    ACCTCCATAG ATTTCCCTGA CGAAACCCTG TCATTCATCA AATCCCATCC GCTAATGGAC
    GCTGCTGTAC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCTGTTGA CCACACTGCT GGGCCCCATC AGAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTGCTTAAA GTTTTGGCAA AGACCAGCCC TTTCTCTTTG
    AATGACAGTG TATTACTGGA AGAGATTGAA GCATACAACC AAGCAAAGTG CAATGCTGAG
    AATGAGGAAG ACAGAAGGGT CATCTCCTTA CAGTTGGATA AAGACCATCA CGCTGTGTAC
    GTGGCATTCT CTAGCTGCGT CATCCGCCTC CCTCTCAGCC GTTGCGAGCG TTACGGATCC
    TGTAAGAAGT CTTGTGTTGC ATCTCGGGAC CCATACTGTG GCTGGTTAAG CCAGGGGGCC
    TGTGGCAGAG TGACCCCAGG GATGCTCGCT GGAGGATATG AACAAGACAC AGAGTACGGC
    AACACGGCCC ACCTAGGGGA CTGCCATGAA ATTTTGCCTA CTTCAACTAC ACCAGATTAC
    AAAATATTTG GCGGTCCAAC ATCTGGTGTG CGATGGGAAG TCCAATCGGG AGAGTCCAAC
    CAGATGGTCC ACATGAACGT CCTCATCACC TGTGTCTTTG CCGCTTTTGT TTTGGGTGCG
    TTCATTGCAG GGGTGGCAGT ATACTGCTAT CGTGACATGT TTGTTCGGAA AAACAGAAAG
    ATTCATAAGG ATGCAGAATC TGCCCAGTCG TGCACAGACT CCAGTGGAAG TTTTGCCAAA
    CTGAATGGTC TGTTTGATAG CCCGGTCAAG GAATATCAAC AGAATATCGA TTCTCCCAAA
    TTATACAGTA ACCTGCTGAC TAGTCGGAAA GAGCTGCCAC CCAATGGAGA TACCAAATCC
    ATGGTGATGG ACCATCGAGG CCAACCTCCC GAACTGGCTG CTCTCCCCAC GCCTGAGTCA
    ACTCCTGTGC TTCACCAGAA GACCCTGCAG GCCATGAAGA GCCACTCAGA TAAGGCCCAT
    GGCCATGGGG CTTCAAGGAA AGAAACCTCA CAGTTTTTCC CATCTAGTCC ACCACCCCAT
    TCCCCATTAA GTCATGGGCA TATCCCCAGT GCCATTGTTC TTCCTAATGC TACTCATGAC
    TACAACACGT CTTTCTCAAA CTCCAATGCT CACAAAGCTG AAAAGAAGCT TCAAAACATT
    GACCACCCAC TTACAAAGTC ATCCAGTAAA AGAGATCACC GGCGTTCTGT GGATTCCAGA
    AATACCCTCA ATGATCTCCT GAAGCATTTA AATGACCCAA ACAGCAACCC CAAAGCCATC
    ATGGGAGATA TCCAGATGGC CCACCAGACC CTAATGCTGG ATCCTGTGGG ACCTATGTCT
    GAGGTCCCGC CCAAGGTCCC TAACCGGGAG GCATCCCTAT ACTCTCCTCC TTCAACTCTC
    CCCAGAAATA GCCCAACCAA GCGAGTGGAT GTCCCCACCA CTCCTGGGGT CCCAATGACT
    TCTCTGGAAA GACAAAGGGG TTACCACAAA AATTCCTCCC AGAGGCACTC TATATCTGCT
    ATGCCTAAAA ACTTAAACTC ACCAAATGGT GTTTTGTTAT CCAGACAGCC TAGCATGAAC
    CGTGGTGGGT ATGTGCCCAC CTCCACTGGG GCAAAGGTGG ACTATATTCA GGGAGCACCA
    GTGAGTGTTC ATCTGCAGCC TTCCCTCTCC AGACAGAGCA GCTATACCAG TAATGGCACC
    CTTCCTAGGA CGGGACTAAA GAGGACACCG TCCTTAAAAC CTGATGTGCC ACCAAAGCCT
    TCATTTGTTC CTCAAACCAC ATCTGTCAGA CCACTGAACA AATACACTTA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe28818
    Clone ID Related Accession (Same CDS sequence) XM_003640053.3 , XM_003640053.4
    Accession Version XM_003640053.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3054bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product semaphorin-6D isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003640053.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGGTTCT TCCTGCTTTG TGCCTACATG CTGCTCCTGA TGATTTCCCA GCTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTC AATACTGTTG ATTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AATGAATCCC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAGTCCCCAA GACAGAAGTA ATACCAAGCA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ACCGAGAAAA CTGTGCTATG AAGGGCAAAC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTCCC AAGAAATGAT GAGATGGTTT TTGTATGTGG TACCAATGCA
    TTCAATCCCA TGTGCAGATA CTATAGGTTG AATACCTTAG AGTATGATGG AGAGGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTAT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC CCTTCGTACA ATAAAATATG ATTCCAAATG GATAAAAGAG
    CCACATTTTC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAATAATTT AGGCAAGGCT GTATATTCCC GTGTGGCCCG CATATGTAAA
    AATGACATGG GTGGCTCGCA GCGGGTCCTG GAGAAACACT GGACTTCATT TCTGAAGGCT
    CGGCTTAACT GCTCTGTCCC CGGAGATTCG TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACGGACATCA TACAAATCAA TGGCATCCCC ACTGTGGTCG GGGTGTTTAC CACACAGCTC
    AACAGCATTC CTGGGTCTGC AGTGTGTGCA TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGCT TTAAAGAACA GAAAACCCCA GATTCTGTTT GGACAGCAGT CCCTGAAGAC
    AAAGTACCCA AGCCAAGGCC TGGCTGTTGT GCAAAGCATG GCCTTGCCGA AGCTTATAAA
    ACCTCCATAG ATTTCCCTGA CGAAACCCTG TCATTCATCA AATCCCATCC GCTAATGGAC
    GCTGCTGTAC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCTGTTGA CCACACTGCT GGGCCCCATC AGAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTGCTTAAA GTTTTGGCAA AGACCAGCCC TTTCTCTTTG
    AATGACAGTG TATTACTGGA AGAGATTGAA GCATACAACC AAGCAAAGTG CAATGCTGAG
    AATGAGGAAG ACAGAAGGGT CATCTCCTTA CAGTTGGATA AAGACCATCA CGCTGTGTAC
    GTGGCATTCT CTAGCTGCGT CATCCGCCTC CCTCTCAGCC GTTGCGAGCG TTACGGATCC
    TGTAAGAAGT CTTGTGTTGC ATCTCGGGAC CCATACTGTG GCTGGTTAAG CCAGGGGGCC
    TGTGGCAGAG TGACCCCAGG GATGCTCGCT GGAGGATATG AACAAGACAC AGAGTACGGC
    AACACGGCCC ACCTAGGGGA CTGCCATGAA ATTTTGCCTA CTTCAACTAC ACCAGATTAC
    AAAATATTTG GCGGTCCAAC ATCTGGTGTG CGATGGGAAG TCCAATCGGG AGAGTCCAAC
    CAGATGGTCC ACATGAACGT CCTCATCACC TGTGTCTTTG CCGCTTTTGT TTTGGGTGCG
    TTCATTGCAG GGGTGGCAGT ATACTGCTAT CGTGACATGT TTGTTCGGAA AAACAGAAAG
    ATTCATAAGG ATGCAGAATC TGCCCAGTCG TGCACAGACT CCAGTGGAAG TTTTGCCAAA
    CTGAATGGTC TGTTTGATAG CCCGGTCAAG GAATATCAAC AGAATATCGA TTCTCCCAAA
    TTATACAGTA ACCTGCTGAC TAGTCGGAAA GAGCTGCCAC CCAATGGAGA TACCAAATCC
    ATGGTGATGG ACCATCGAGG CCAACCTCCC GAACTGGCTG CTCTCCCCAC GCCTGAGTCA
    ACTCCTGTGC TTCACCAGAA GACCCTGCAG GCCATGAAGA GCCACTCAGA TAAGGCCCAT
    GGCCATGGGG CTTCAAGGAA AGAAACCTCA CAGTTTTTCC CATCTAGTCC ACCACCCCAT
    TCCCCATTAA GTCATGGGCA TATCCCCAGT GCCATTGTTC TTCCTAATGC TACTCATGAC
    TACAACACGT CTTTCTCAAA CTCCAATGCT CACAAAGCTG AAAAGAAGCT TCAAAACATT
    GACCACCCAC TTACAAAGTC ATCCAGTAAA AGAGATCACC GGCGTTCTGT GGATTCCAGA
    AATACCCTCA ATGATCTCCT GAAGCATTTA AATGACCCAA ACAGCAACCC CAAAGCCATC
    ATGGGAGATA TCCAGATGGC CCACCAGACC CTAATGCTGG ATCCTGTGGG ACCTATGTCT
    GAGGTCCCGC CCAAGGTCCC TAACCGGGAG GCATCCCTAT ACTCTCCTCC TTCAACTCTC
    CCCAGAAATA GCCCAACCAA GCGAGTGGAT GTCCCCACCA CTCCTGGGGT CCCAATGACT
    TCTCTGGAAA GACAAAGGGG TTACCACAAA AATTCCTCCC AGAGGCACTC TATATCTGCT
    ATGCCTAAAA ACTTAAACTC ACCAAATGGT GTTTTGTTAT CCAGACAGCC TAGCATGAAC
    CGTGGTGGGT ATGTGCCCAC CTCCACTGGG GCAAAGGTGG ACTATATTCA GGGAGCACCA
    GTGAGTGTTC ATCTGCAGCC TTCCCTCTCC AGACAGAGCA GCTATACCAG TAATGGCACC
    CTTCCTAGGA CGGGACTAAA GAGGACACCG TCCTTAAAAC CTGATGTGCC ACCAAAGCCT
    TCATTTGTTC CTCAAACCAC ATCTGTCAGA CCACTGAACA AATACACTTA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003640101.1
    CDS287..3340
    Translation

    Target ORF information:

    RefSeq Version XM_003640053.4
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris semaphorin 6D (SEMA6D), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003640053.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGGTTCT TCCTGCTTTG TGCCTACATG CTGCTCCTGA TGATTTCCCA GCTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTC AATACTGTTG ATTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AATGAATCCC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAGTCCCCAA GACAGAAGTA ATACCAAGCA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ACCGAGAAAA CTGTGCTATG AAGGGCAAAC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTCCC AAGAAATGAT GAGATGGTTT TTGTATGTGG TACCAATGCA
    TTCAATCCCA TGTGCAGATA CTATAGGTTG AATACCTTAG AGTATGATGG AGAGGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTAT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC CCTTCGTACA ATAAAATATG ATTCCAAATG GATAAAAGAG
    CCACATTTTC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAATAATTT AGGCAAGGCT GTATATTCCC GTGTGGCCCG CATATGTAAA
    AATGACATGG GTGGCTCGCA GCGGGTCCTG GAGAAACACT GGACTTCATT TCTGAAGGCT
    CGGCTTAACT GCTCTGTCCC CGGAGATTCG TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACGGACATCA TACAAATCAA TGGCATCCCC ACTGTGGTCG GGGTGTTTAC CACACAGCTC
    AACAGCATTC CTGGGTCTGC AGTGTGTGCA TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGCT TTAAAGAACA GAAAACCCCA GATTCTGTTT GGACAGCAGT CCCTGAAGAC
    AAAGTACCCA AGCCAAGGCC TGGCTGTTGT GCAAAGCATG GCCTTGCCGA AGCTTATAAA
    ACCTCCATAG ATTTCCCTGA CGAAACCCTG TCATTCATCA AATCCCATCC GCTAATGGAC
    GCTGCTGTAC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCTGTTGA CCACACTGCT GGGCCCCATC AGAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTGCTTAAA GTTTTGGCAA AGACCAGCCC TTTCTCTTTG
    AATGACAGTG TATTACTGGA AGAGATTGAA GCATACAACC AAGCAAAGTG CAATGCTGAG
    AATGAGGAAG ACAGAAGGGT CATCTCCTTA CAGTTGGATA AAGACCATCA CGCTGTGTAC
    GTGGCATTCT CTAGCTGCGT CATCCGCCTC CCTCTCAGCC GTTGCGAGCG TTACGGATCC
    TGTAAGAAGT CTTGTGTTGC ATCTCGGGAC CCATACTGTG GCTGGTTAAG CCAGGGGGCC
    TGTGGCAGAG TGACCCCAGG GATGCTCGCT GGAGGATATG AACAAGACAC AGAGTACGGC
    AACACGGCCC ACCTAGGGGA CTGCCATGAA ATTTTGCCTA CTTCAACTAC ACCAGATTAC
    AAAATATTTG GCGGTCCAAC ATCTGGTGTG CGATGGGAAG TCCAATCGGG AGAGTCCAAC
    CAGATGGTCC ACATGAACGT CCTCATCACC TGTGTCTTTG CCGCTTTTGT TTTGGGTGCG
    TTCATTGCAG GGGTGGCAGT ATACTGCTAT CGTGACATGT TTGTTCGGAA AAACAGAAAG
    ATTCATAAGG ATGCAGAATC TGCCCAGTCG TGCACAGACT CCAGTGGAAG TTTTGCCAAA
    CTGAATGGTC TGTTTGATAG CCCGGTCAAG GAATATCAAC AGAATATCGA TTCTCCCAAA
    TTATACAGTA ACCTGCTGAC TAGTCGGAAA GAGCTGCCAC CCAATGGAGA TACCAAATCC
    ATGGTGATGG ACCATCGAGG CCAACCTCCC GAACTGGCTG CTCTCCCCAC GCCTGAGTCA
    ACTCCTGTGC TTCACCAGAA GACCCTGCAG GCCATGAAGA GCCACTCAGA TAAGGCCCAT
    GGCCATGGGG CTTCAAGGAA AGAAACCTCA CAGTTTTTCC CATCTAGTCC ACCACCCCAT
    TCCCCATTAA GTCATGGGCA TATCCCCAGT GCCATTGTTC TTCCTAATGC TACTCATGAC
    TACAACACGT CTTTCTCAAA CTCCAATGCT CACAAAGCTG AAAAGAAGCT TCAAAACATT
    GACCACCCAC TTACAAAGTC ATCCAGTAAA AGAGATCACC GGCGTTCTGT GGATTCCAGA
    AATACCCTCA ATGATCTCCT GAAGCATTTA AATGACCCAA ACAGCAACCC CAAAGCCATC
    ATGGGAGATA TCCAGATGGC CCACCAGACC CTAATGCTGG ATCCTGTGGG ACCTATGTCT
    GAGGTCCCGC CCAAGGTCCC TAACCGGGAG GCATCCCTAT ACTCTCCTCC TTCAACTCTC
    CCCAGAAATA GCCCAACCAA GCGAGTGGAT GTCCCCACCA CTCCTGGGGT CCCAATGACT
    TCTCTGGAAA GACAAAGGGG TTACCACAAA AATTCCTCCC AGAGGCACTC TATATCTGCT
    ATGCCTAAAA ACTTAAACTC ACCAAATGGT GTTTTGTTAT CCAGACAGCC TAGCATGAAC
    CGTGGTGGGT ATGTGCCCAC CTCCACTGGG GCAAAGGTGG ACTATATTCA GGGAGCACCA
    GTGAGTGTTC ATCTGCAGCC TTCCCTCTCC AGACAGAGCA GCTATACCAG TAATGGCACC
    CTTCCTAGGA CGGGACTAAA GAGGACACCG TCCTTAAAAC CTGATGTGCC ACCAAAGCCT
    TCATTTGTTC CTCAAACCAC ATCTGTCAGA CCACTGAACA AATACACTTA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe28819
    Clone ID Related Accession (Same CDS sequence) XM_003640051.3 , XM_003640051.4
    Accession Version XM_003640051.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3036bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product semaphorin-6D isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003640051.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGGTTCT TCCTGCTTTG TGCCTACATG CTGCTCCTGA TGATTTCCCA GCTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTC AATACTGTTG ATTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AATGAATCCC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAGTCCCCAA GACAGAAGTA ATACCAAGCA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ACCGAGAAAA CTGTGCTATG AAGGGCAAAC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTCCC AAGAAATGAT GAGATGGTTT TTGTATGTGG TACCAATGCA
    TTCAATCCCA TGTGCAGATA CTATAGGTTG AATACCTTAG AGTATGATGG AGAGGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTAT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC CCTTCGTACA ATAAAATATG ATTCCAAATG GATAAAAGAG
    CCACATTTTC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAATAATTT AGGCAAGGCT GTATATTCCC GTGTGGCCCG CATATGTAAA
    AATGACATGG GTGGCTCGCA GCGGGTCCTG GAGAAACACT GGACTTCATT TCTGAAGGCT
    CGGCTTAACT GCTCTGTCCC CGGAGATTCG TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACGGACATCA TACAAATCAA TGGCATCCCC ACTGTGGTCG GGGTGTTTAC CACACAGCTC
    AACAGCATTC CTGGGTCTGC AGTGTGTGCA TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGCT TTAAAGAACA GAAAACCCCA GATTCTGTTT GGACAGCAGT CCCTGAAGAC
    AAAGTACCCA AGCCAAGGCC TGGCTGTTGT GCAAAGCATG GCCTTGCCGA AGCTTATAAA
    ACCTCCATAG ATTTCCCTGA CGAAACCCTG TCATTCATCA AATCCCATCC GCTAATGGAC
    GCTGCTGTAC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCTGTTGA CCACACTGCT GGGCCCCATC AGAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTGCTTAAA GTTTTGGCAA AGACCAGCCC TTTCTCTTTG
    AATGACAGTG TATTACTGGA AGAGATTGAA GCATACAACC AAGCAAAGTG CAATGCTGAG
    AATGAGGAAG ACAGAAGGGT CATCTCCTTA CAGTTGGATA AAGACCATCA CGCTGTGTAC
    GTGGCATTCT CTAGCTGCGT CATCCGCCTC CCTCTCAGCC GTTGCGAGCG TTACGGATCC
    TGTAAGAAGT CTTGTGTTGC ATCTCGGGAC CCATACTGTG GCTGGTTAAG CCAGGGGGCC
    TGTGGCAGAG TGACCCCAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATATGA ACAAGACACA GAGTACGGCA ACACGGCCCA CCTAGGGGAC
    TGCCATGGTG TGCGATGGGA AGTCCAATCG GGAGAGTCCA ACCAGATGGT CCACATGAAC
    GTCCTCATCA CCTGTGTCTT TGCCGCTTTT GTTTTGGGTG CGTTCATTGC AGGGGTGGCA
    GTATACTGCT ATCGTGACAT GTTTGTTCGG AAAAACAGAA AGATTCATAA GGATGCAGAA
    TCTGCCCAGT CGTGCACAGA CTCCAGTGGA AGTTTTGCCA AACTGAATGG TCTGTTTGAT
    AGCCCGGTCA AGGAATATCA ACAGAATATC GATTCTCCCA AATTATACAG TAACCTGCTG
    ACTAGTCGGA AAGAGCTGCC ACCCAATGGA GATACCAAAT CCATGGTGAT GGACCATCGA
    GGCCAACCTC CCGAACTGGC TGCTCTCCCC ACGCCTGAGT CAACTCCTGT GCTTCACCAG
    AAGACCCTGC AGGCCATGAA GAGCCACTCA GATAAGGCCC ATGGCCATGG GGCTTCAAGG
    AAAGAAACCT CACAGTTTTT CCCATCTAGT CCACCACCCC ATTCCCCATT AAGTCATGGG
    CATATCCCCA GTGCCATTGT TCTTCCTAAT GCTACTCATG ACTACAACAC GTCTTTCTCA
    AACTCCAATG CTCACAAAGC TGAAAAGAAG CTTCAAAACA TTGACCACCC ACTTACAAAG
    TCATCCAGTA AAAGAGATCA CCGGCGTTCT GTGGATTCCA GAAATACCCT CAATGATCTC
    CTGAAGCATT TAAATGACCC AAACAGCAAC CCCAAAGCCA TCATGGGAGA TATCCAGATG
    GCCCACCAGA CCCTAATGCT GGATCCTGTG GGACCTATGT CTGAGGTCCC GCCCAAGGTC
    CCTAACCGGG AGGCATCCCT ATACTCTCCT CCTTCAACTC TCCCCAGAAA TAGCCCAACC
    AAGCGAGTGG ATGTCCCCAC CACTCCTGGG GTCCCAATGA CTTCTCTGGA AAGACAAAGG
    GGTTACCACA AAAATTCCTC CCAGAGGCAC TCTATATCTG CTATGCCTAA AAACTTAAAC
    TCACCAAATG GTGTTTTGTT ATCCAGACAG CCTAGCATGA ACCGTGGTGG GTATGTGCCC
    ACCTCCACTG GGGCAAAGGT GGACTATATT CAGGGAGCAC CAGTGAGTGT TCATCTGCAG
    CCTTCCCTCT CCAGACAGAG CAGCTATACC AGTAATGGCA CCCTTCCTAG GACGGGACTA
    AAGAGGACAC CGTCCTTAAA ACCTGATGTG CCACCAAAGC CTTCATTTGT TCCTCAAACC
    ACATCTGTCA GACCACTGAA CAAATACACT TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003640099.1
    CDS314..3349
    Translation

    Target ORF information:

    RefSeq Version XM_003640051.3
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (SEMA6D), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003640051.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGGTTCT TCCTGCTTTG TGCCTACATG CTGCTCCTGA TGATTTCCCA GCTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTC AATACTGTTG ATTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AATGAATCCC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAGTCCCCAA GACAGAAGTA ATACCAAGCA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ACCGAGAAAA CTGTGCTATG AAGGGCAAAC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTCCC AAGAAATGAT GAGATGGTTT TTGTATGTGG TACCAATGCA
    TTCAATCCCA TGTGCAGATA CTATAGGTTG AATACCTTAG AGTATGATGG AGAGGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTAT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC CCTTCGTACA ATAAAATATG ATTCCAAATG GATAAAAGAG
    CCACATTTTC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAATAATTT AGGCAAGGCT GTATATTCCC GTGTGGCCCG CATATGTAAA
    AATGACATGG GTGGCTCGCA GCGGGTCCTG GAGAAACACT GGACTTCATT TCTGAAGGCT
    CGGCTTAACT GCTCTGTCCC CGGAGATTCG TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACGGACATCA TACAAATCAA TGGCATCCCC ACTGTGGTCG GGGTGTTTAC CACACAGCTC
    AACAGCATTC CTGGGTCTGC AGTGTGTGCA TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGCT TTAAAGAACA GAAAACCCCA GATTCTGTTT GGACAGCAGT CCCTGAAGAC
    AAAGTACCCA AGCCAAGGCC TGGCTGTTGT GCAAAGCATG GCCTTGCCGA AGCTTATAAA
    ACCTCCATAG ATTTCCCTGA CGAAACCCTG TCATTCATCA AATCCCATCC GCTAATGGAC
    GCTGCTGTAC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCTGTTGA CCACACTGCT GGGCCCCATC AGAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTGCTTAAA GTTTTGGCAA AGACCAGCCC TTTCTCTTTG
    AATGACAGTG TATTACTGGA AGAGATTGAA GCATACAACC AAGCAAAGTG CAATGCTGAG
    AATGAGGAAG ACAGAAGGGT CATCTCCTTA CAGTTGGATA AAGACCATCA CGCTGTGTAC
    GTGGCATTCT CTAGCTGCGT CATCCGCCTC CCTCTCAGCC GTTGCGAGCG TTACGGATCC
    TGTAAGAAGT CTTGTGTTGC ATCTCGGGAC CCATACTGTG GCTGGTTAAG CCAGGGGGCC
    TGTGGCAGAG TGACCCCAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATATGA ACAAGACACA GAGTACGGCA ACACGGCCCA CCTAGGGGAC
    TGCCATGGTG TGCGATGGGA AGTCCAATCG GGAGAGTCCA ACCAGATGGT CCACATGAAC
    GTCCTCATCA CCTGTGTCTT TGCCGCTTTT GTTTTGGGTG CGTTCATTGC AGGGGTGGCA
    GTATACTGCT ATCGTGACAT GTTTGTTCGG AAAAACAGAA AGATTCATAA GGATGCAGAA
    TCTGCCCAGT CGTGCACAGA CTCCAGTGGA AGTTTTGCCA AACTGAATGG TCTGTTTGAT
    AGCCCGGTCA AGGAATATCA ACAGAATATC GATTCTCCCA AATTATACAG TAACCTGCTG
    ACTAGTCGGA AAGAGCTGCC ACCCAATGGA GATACCAAAT CCATGGTGAT GGACCATCGA
    GGCCAACCTC CCGAACTGGC TGCTCTCCCC ACGCCTGAGT CAACTCCTGT GCTTCACCAG
    AAGACCCTGC AGGCCATGAA GAGCCACTCA GATAAGGCCC ATGGCCATGG GGCTTCAAGG
    AAAGAAACCT CACAGTTTTT CCCATCTAGT CCACCACCCC ATTCCCCATT AAGTCATGGG
    CATATCCCCA GTGCCATTGT TCTTCCTAAT GCTACTCATG ACTACAACAC GTCTTTCTCA
    AACTCCAATG CTCACAAAGC TGAAAAGAAG CTTCAAAACA TTGACCACCC ACTTACAAAG
    TCATCCAGTA AAAGAGATCA CCGGCGTTCT GTGGATTCCA GAAATACCCT CAATGATCTC
    CTGAAGCATT TAAATGACCC AAACAGCAAC CCCAAAGCCA TCATGGGAGA TATCCAGATG
    GCCCACCAGA CCCTAATGCT GGATCCTGTG GGACCTATGT CTGAGGTCCC GCCCAAGGTC
    CCTAACCGGG AGGCATCCCT ATACTCTCCT CCTTCAACTC TCCCCAGAAA TAGCCCAACC
    AAGCGAGTGG ATGTCCCCAC CACTCCTGGG GTCCCAATGA CTTCTCTGGA AAGACAAAGG
    GGTTACCACA AAAATTCCTC CCAGAGGCAC TCTATATCTG CTATGCCTAA AAACTTAAAC
    TCACCAAATG GTGTTTTGTT ATCCAGACAG CCTAGCATGA ACCGTGGTGG GTATGTGCCC
    ACCTCCACTG GGGCAAAGGT GGACTATATT CAGGGAGCAC CAGTGAGTGT TCATCTGCAG
    CCTTCCCTCT CCAGACAGAG CAGCTATACC AGTAATGGCA CCCTTCCTAG GACGGGACTA
    AAGAGGACAC CGTCCTTAAA ACCTGATGTG CCACCAAAGC CTTCATTTGT TCCTCAAACC
    ACATCTGTCA GACCACTGAA CAAATACACT TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe28819
    Clone ID Related Accession (Same CDS sequence) XM_003640051.3 , XM_003640051.4
    Accession Version XM_003640051.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3036bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product semaphorin-6D isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003640051.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGGTTCT TCCTGCTTTG TGCCTACATG CTGCTCCTGA TGATTTCCCA GCTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTC AATACTGTTG ATTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AATGAATCCC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAGTCCCCAA GACAGAAGTA ATACCAAGCA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ACCGAGAAAA CTGTGCTATG AAGGGCAAAC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTCCC AAGAAATGAT GAGATGGTTT TTGTATGTGG TACCAATGCA
    TTCAATCCCA TGTGCAGATA CTATAGGTTG AATACCTTAG AGTATGATGG AGAGGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTAT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC CCTTCGTACA ATAAAATATG ATTCCAAATG GATAAAAGAG
    CCACATTTTC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAATAATTT AGGCAAGGCT GTATATTCCC GTGTGGCCCG CATATGTAAA
    AATGACATGG GTGGCTCGCA GCGGGTCCTG GAGAAACACT GGACTTCATT TCTGAAGGCT
    CGGCTTAACT GCTCTGTCCC CGGAGATTCG TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACGGACATCA TACAAATCAA TGGCATCCCC ACTGTGGTCG GGGTGTTTAC CACACAGCTC
    AACAGCATTC CTGGGTCTGC AGTGTGTGCA TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGCT TTAAAGAACA GAAAACCCCA GATTCTGTTT GGACAGCAGT CCCTGAAGAC
    AAAGTACCCA AGCCAAGGCC TGGCTGTTGT GCAAAGCATG GCCTTGCCGA AGCTTATAAA
    ACCTCCATAG ATTTCCCTGA CGAAACCCTG TCATTCATCA AATCCCATCC GCTAATGGAC
    GCTGCTGTAC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCTGTTGA CCACACTGCT GGGCCCCATC AGAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTGCTTAAA GTTTTGGCAA AGACCAGCCC TTTCTCTTTG
    AATGACAGTG TATTACTGGA AGAGATTGAA GCATACAACC AAGCAAAGTG CAATGCTGAG
    AATGAGGAAG ACAGAAGGGT CATCTCCTTA CAGTTGGATA AAGACCATCA CGCTGTGTAC
    GTGGCATTCT CTAGCTGCGT CATCCGCCTC CCTCTCAGCC GTTGCGAGCG TTACGGATCC
    TGTAAGAAGT CTTGTGTTGC ATCTCGGGAC CCATACTGTG GCTGGTTAAG CCAGGGGGCC
    TGTGGCAGAG TGACCCCAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATATGA ACAAGACACA GAGTACGGCA ACACGGCCCA CCTAGGGGAC
    TGCCATGGTG TGCGATGGGA AGTCCAATCG GGAGAGTCCA ACCAGATGGT CCACATGAAC
    GTCCTCATCA CCTGTGTCTT TGCCGCTTTT GTTTTGGGTG CGTTCATTGC AGGGGTGGCA
    GTATACTGCT ATCGTGACAT GTTTGTTCGG AAAAACAGAA AGATTCATAA GGATGCAGAA
    TCTGCCCAGT CGTGCACAGA CTCCAGTGGA AGTTTTGCCA AACTGAATGG TCTGTTTGAT
    AGCCCGGTCA AGGAATATCA ACAGAATATC GATTCTCCCA AATTATACAG TAACCTGCTG
    ACTAGTCGGA AAGAGCTGCC ACCCAATGGA GATACCAAAT CCATGGTGAT GGACCATCGA
    GGCCAACCTC CCGAACTGGC TGCTCTCCCC ACGCCTGAGT CAACTCCTGT GCTTCACCAG
    AAGACCCTGC AGGCCATGAA GAGCCACTCA GATAAGGCCC ATGGCCATGG GGCTTCAAGG
    AAAGAAACCT CACAGTTTTT CCCATCTAGT CCACCACCCC ATTCCCCATT AAGTCATGGG
    CATATCCCCA GTGCCATTGT TCTTCCTAAT GCTACTCATG ACTACAACAC GTCTTTCTCA
    AACTCCAATG CTCACAAAGC TGAAAAGAAG CTTCAAAACA TTGACCACCC ACTTACAAAG
    TCATCCAGTA AAAGAGATCA CCGGCGTTCT GTGGATTCCA GAAATACCCT CAATGATCTC
    CTGAAGCATT TAAATGACCC AAACAGCAAC CCCAAAGCCA TCATGGGAGA TATCCAGATG
    GCCCACCAGA CCCTAATGCT GGATCCTGTG GGACCTATGT CTGAGGTCCC GCCCAAGGTC
    CCTAACCGGG AGGCATCCCT ATACTCTCCT CCTTCAACTC TCCCCAGAAA TAGCCCAACC
    AAGCGAGTGG ATGTCCCCAC CACTCCTGGG GTCCCAATGA CTTCTCTGGA AAGACAAAGG
    GGTTACCACA AAAATTCCTC CCAGAGGCAC TCTATATCTG CTATGCCTAA AAACTTAAAC
    TCACCAAATG GTGTTTTGTT ATCCAGACAG CCTAGCATGA ACCGTGGTGG GTATGTGCCC
    ACCTCCACTG GGGCAAAGGT GGACTATATT CAGGGAGCAC CAGTGAGTGT TCATCTGCAG
    CCTTCCCTCT CCAGACAGAG CAGCTATACC AGTAATGGCA CCCTTCCTAG GACGGGACTA
    AAGAGGACAC CGTCCTTAAA ACCTGATGTG CCACCAAAGC CTTCATTTGT TCCTCAAACC
    ACATCTGTCA GACCACTGAA CAAATACACT TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003640099.1
    CDS287..3322
    Translation

    Target ORF information:

    RefSeq Version XM_003640051.4
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris semaphorin 6D (SEMA6D), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003640051.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGGTTCT TCCTGCTTTG TGCCTACATG CTGCTCCTGA TGATTTCCCA GCTGAGGGCA 
    GTCAGCTTTC CTGAAGATGA TGAACCCCTC AATACTGTTG ATTATCACTA TTCAAGGCAA
    TATCCGGTTT TTAGAGGACG CCCTTCAGGC AATGAATCCC AGCACAGGCT GGACTTTCAG
    CTGATGTTGA AAATTCGAGA CACACTTTAT ATTGCTGGCA GGGATCAAGT TTATACAGTA
    AACTTAAATG AAGTCCCCAA GACAGAAGTA ATACCAAGCA AGAAACTGAC ATGGCGGTCA
    AGACAACAGG ACCGAGAAAA CTGTGCTATG AAGGGCAAAC ATAAAGATGA ATGCCACAAC
    TTTATTAAAG TATTTGTCCC AAGAAATGAT GAGATGGTTT TTGTATGTGG TACCAATGCA
    TTCAATCCCA TGTGCAGATA CTATAGGTTG AATACCTTAG AGTATGATGG AGAGGAAATT
    AGTGGCCTGG CAAGATGCCC ATTTGATGCC AGACAAACCA ATGTTGCCCT TTTTGCTGAT
    GGGAAGCTAT ATTCTGCCAC AGTGGCTGAC TTCTTGGCCA GTGATGCTGT TATTTATCGA
    AGCATGGGTG ATGGATCTGC CCTTCGTACA ATAAAATATG ATTCCAAATG GATAAAAGAG
    CCACATTTTC TTCATGCCAT AGAATATGGA AACTATGTCT ATTTCTTCTT TCGAGAAATT
    GCTGTAGAAC ATAATAATTT AGGCAAGGCT GTATATTCCC GTGTGGCCCG CATATGTAAA
    AATGACATGG GTGGCTCGCA GCGGGTCCTG GAGAAACACT GGACTTCATT TCTGAAGGCT
    CGGCTTAACT GCTCTGTCCC CGGAGATTCG TTTTTCTACT TTGATGTTCT GCAGTCTATT
    ACGGACATCA TACAAATCAA TGGCATCCCC ACTGTGGTCG GGGTGTTTAC CACACAGCTC
    AACAGCATTC CTGGGTCTGC AGTGTGTGCA TTTAGCATGG ATGACATTGA AAAAGTATTC
    AAAGGACGCT TTAAAGAACA GAAAACCCCA GATTCTGTTT GGACAGCAGT CCCTGAAGAC
    AAAGTACCCA AGCCAAGGCC TGGCTGTTGT GCAAAGCATG GCCTTGCCGA AGCTTATAAA
    ACCTCCATAG ATTTCCCTGA CGAAACCCTG TCATTCATCA AATCCCATCC GCTAATGGAC
    GCTGCTGTAC CACCCATTGC CGATGAGCCC TGGTTCACAA AGACTCGGAT CAGGTACAGA
    CTGACGGCCA TCGCTGTTGA CCACACTGCT GGGCCCCATC AGAACTACAC AGTCATCTTT
    GTTGGCTCTG AAGCTGGCGT GGTGCTTAAA GTTTTGGCAA AGACCAGCCC TTTCTCTTTG
    AATGACAGTG TATTACTGGA AGAGATTGAA GCATACAACC AAGCAAAGTG CAATGCTGAG
    AATGAGGAAG ACAGAAGGGT CATCTCCTTA CAGTTGGATA AAGACCATCA CGCTGTGTAC
    GTGGCATTCT CTAGCTGCGT CATCCGCCTC CCTCTCAGCC GTTGCGAGCG TTACGGATCC
    TGTAAGAAGT CTTGTGTTGC ATCTCGGGAC CCATACTGTG GCTGGTTAAG CCAGGGGGCC
    TGTGGCAGAG TGACCCCAGG GATGCTGCTG TTAACCGAAG ACTTCTTTGC TTTCCATAAC
    CACAGCGCTG GAGGATATGA ACAAGACACA GAGTACGGCA ACACGGCCCA CCTAGGGGAC
    TGCCATGGTG TGCGATGGGA AGTCCAATCG GGAGAGTCCA ACCAGATGGT CCACATGAAC
    GTCCTCATCA CCTGTGTCTT TGCCGCTTTT GTTTTGGGTG CGTTCATTGC AGGGGTGGCA
    GTATACTGCT ATCGTGACAT GTTTGTTCGG AAAAACAGAA AGATTCATAA GGATGCAGAA
    TCTGCCCAGT CGTGCACAGA CTCCAGTGGA AGTTTTGCCA AACTGAATGG TCTGTTTGAT
    AGCCCGGTCA AGGAATATCA ACAGAATATC GATTCTCCCA AATTATACAG TAACCTGCTG
    ACTAGTCGGA AAGAGCTGCC ACCCAATGGA GATACCAAAT CCATGGTGAT GGACCATCGA
    GGCCAACCTC CCGAACTGGC TGCTCTCCCC ACGCCTGAGT CAACTCCTGT GCTTCACCAG
    AAGACCCTGC AGGCCATGAA GAGCCACTCA GATAAGGCCC ATGGCCATGG GGCTTCAAGG
    AAAGAAACCT CACAGTTTTT CCCATCTAGT CCACCACCCC ATTCCCCATT AAGTCATGGG
    CATATCCCCA GTGCCATTGT TCTTCCTAAT GCTACTCATG ACTACAACAC GTCTTTCTCA
    AACTCCAATG CTCACAAAGC TGAAAAGAAG CTTCAAAACA TTGACCACCC ACTTACAAAG
    TCATCCAGTA AAAGAGATCA CCGGCGTTCT GTGGATTCCA GAAATACCCT CAATGATCTC
    CTGAAGCATT TAAATGACCC AAACAGCAAC CCCAAAGCCA TCATGGGAGA TATCCAGATG
    GCCCACCAGA CCCTAATGCT GGATCCTGTG GGACCTATGT CTGAGGTCCC GCCCAAGGTC
    CCTAACCGGG AGGCATCCCT ATACTCTCCT CCTTCAACTC TCCCCAGAAA TAGCCCAACC
    AAGCGAGTGG ATGTCCCCAC CACTCCTGGG GTCCCAATGA CTTCTCTGGA AAGACAAAGG
    GGTTACCACA AAAATTCCTC CCAGAGGCAC TCTATATCTG CTATGCCTAA AAACTTAAAC
    TCACCAAATG GTGTTTTGTT ATCCAGACAG CCTAGCATGA ACCGTGGTGG GTATGTGCCC
    ACCTCCACTG GGGCAAAGGT GGACTATATT CAGGGAGCAC CAGTGAGTGT TCATCTGCAG
    CCTTCCCTCT CCAGACAGAG CAGCTATACC AGTAATGGCA CCCTTCCTAG GACGGGACTA
    AAGAGGACAC CGTCCTTAAA ACCTGATGTG CCACCAAAGC CTTCATTTGT TCCTCAAACC
    ACATCTGTCA GACCACTGAA CAAATACACT TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.