AGO1 cDNA ORF clone, Macaca mulatta(Rhesus monkey)

Gene
Clones
gRNAs

Summary

Gene Symbol	AGO1
Entrez Gene ID	712398
Full Name	argonaute 1, RISC catalytic component
Synonyms	EIF2C1
Gene Type	protein-coding
Organism	Macaca mulatta(Rhesus monkey)
Summary	This gene encodes a member of the argonaute family of proteins, which associate with small RNAs and have important roles in RNA interference (RNAi) and RNA silencing. This protein binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses translation of mRNAs that are complementary to them. It is also involved in transcriptional gene silencing (TGS) of promoter regions that are complementary to bound short antigene RNAs (agRNAs), as well as in the degradation of miRNA-bound mRNA targets. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA could give rise to an additional C-terminally extended isoform by use of an alternative in-frame translation termination codon. [provided by RefSeq, Feb 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001320502.1	NP_001307431.1	protein argonaute-1 isoform 1x
NM_001320501.1	NP_001307430.1	protein argonaute-1 isoform 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

A new rhesus macaque assembly and annotation for next-generation sequencing analyses.
Zimin AV, Cornish AS, Maudhoo MD, Gibbs RM, Zhang X, Pandey S, Meehan DT, Wipfler K, Bosinger SE, Johnson ZP, Tharp GK, Mar?ais G, Roberts M, Ferguson B, Fox HS, Treangen T, Salzberg SL, Yorke JA, Norgren RB Jr
Biology direct9(1)20(2014 Oct)

Programmed translational readthrough generates antiangiogenic VEGF-Ax.
Eswarappa SM, Potdar AA, Koch WJ, Fan Y, Vasu K, Lindner D, Willard B, Graham LM, DiCorleto PE, Fox PL
Cell157(7)1605-18(2014 Jun)

Misreading of termination codons in eukaryotes by natural nonsense suppressor tRNAs.
Beier H, Grimm M
Nucleic acids research29(23)4767-82(2001 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AGO1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AGO1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMb57533	NM_001320502.1 Latest version!	Macaca mulatta argonaute RISC component 1 (AGO1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OMb57534	NM_001320501.1 Latest version!	Macaca mulatta argonaute RISC component 1 (AGO1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AGO1 ( NM_001320502.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> NP_001307431.1 Macaca mulatta argonaute RISC component 1 (AGO1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMb57533
Clone ID Related Accession (Same CDS sequence)	NM_001320502.1
Accession Version	NM_001320502.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-30
Organism	Macaca mulatta(Rhesus monkey)
Product	protein argonaute-1 isoform 1x
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from JU475150.1, JV540982.1 and JV578265.1. Transcript Variant: This variant (1) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon, while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the longer, C-terminally extended isoform (1x). As the UGA stop codon has been reported to specify several alternative amino acids (tryptophan, cysteine, arginine and serine), its location in the longer isoform is denoted by an 'X'. ##Evidence-Data-START## Transcript exon combination :: JU475150.1, JV703716.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA4688315, SAMEA4688316 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## stop codon readthrough :: inferred from conservation ##RefSeq-Attributes-END##

ATGGAAGCGG GACCCTCGGG AGCAGCTGCG GGCGCTTACC TGCCCCCCCT GCAGCAGGTG 
TTCCAGGCAC CTCGCCGGCC TGGCATTGGC ACTGTGGGGA AACCAATCAA GCTCCTGGCC 
AATTACTTTG AGGTGGACAT CCCTAAGATC GACGTGTACC ACTACGAGGT GGACATCAAG 
CCGGATAAGT GTCCCCGTAG AGTCAACCGG GAAGTGGTGG AATACATGGT CCAGCATTTC 
AAGCCTCAGA TCTTTGGTGA TCGCAAGCCT GTGTATGATG GAAAGAAGAA CATTTACACT 
GTCACAGCAC TGCCCATTGG CAACGAACGG GTCGACTTTG AGGTGACAAT CCCTGGGGAA 
GGGAAGGATC GAATCTTTAA GGTCTCCATC AAGTGGCTAG CCATTGTGAG CTGGCGAATG 
CTGCATGAGG CCCTGGTCAG CGGCCAGATC CCTGTTCCCT TGGAGTCTGT GCAAGCCCTG 
GATGTGGCCA TGAGGCACCT GGCATCCATG AGGTACACCC CTGTGGGCCG CTCCTTCTTC 
TCACCGCCTG AGGGCTACTA CCACCCGCTG GGGGGTGGGC GCGAGGTCTG GTTCGGCTTT 
CACCAGTCTG TGCGCCCTGC CATGTGGAAG ATGATGCTCA ACATTGATGT CTCAGCCACT 
GCCTTCTATA AGGCGCAGCC AGTGATTGAG TTCATGTGTG AGGTGCTGGA CATCAGGAAC 
ATAGATGAGC AGCCCAAGCC CCTCACGGAC TCTCAGCGTG TTCGCTTCAC CAAGGAGATC 
AAGGGCCTGA AGGTGGAAGT CACCCACTGT GGACAGATGA AGAGGAAATA CCGCGTGTGT 
AATGTTACCC GTCGCCCTGC TAGCCATCAG ACATTCCCCT TACAGCTGGA GAGTGGACAG 
ACTGTGGAGT GCACAGTGGC ACAGTATTTC AAGCAGAAAT ATAACCTTCA GCTCAAGTAT 
CCCCATCTGC CCTGCCTACA AGTTGGCCAG GAACAAAAGC ATACCTACCT TCCCCTAGAG 
GTCTGTAACA TTGTGGCTGG GCAACGCTGT ATTAAAAAGC TGACCGACAA CCAGACTTCG 
ACCATGATAA AGGCCACAGC TAGATCCGCT CCAGACAGAC AGGAGGAGAT CAGTCGCCTG 
ATGAAGAATG CCAGCTACAA CTTAGATCCC TACATCCAGG AATTTGGGAT CAAAGTGAAG 
GATGACATGA CGGAGGTGAC AGGGCGAGTG CTGCCGGCGC CCATCTTGCA GTACGGCGGC 
CGGAACCGGG CCATTGCCAC ACCCAATCAG GGTGTCTGGG ACATGCGGGG GAAACAGTTC 
TACAATGGGA TTGAGATCAA AGTCTGGGCC ATCGCCTGCT TCGCACCCCA AAAACAGTGT 
CGAGAAGAGG TGCTCAAGAA CTTCACAGAC CAGCTGCGGA AGATTTCCAA GGATGCGGGG 
ATGCCTATCC AGGGTCAGCC TTGTTTCTGC AAATATGCCC AGGGGGCAGA CAGCGTGGAG 
CCTATGTTCC GGCATCTCAA GAACACCTAC TCAGGGCTGC AGCTCATTAT TGTCATCCTG 
CCAGGGAAGA CGCCAGTGTA TGCTGAGGTG AAACGTGTTG GAGATACACT CTTGGGAATG 
GCTACACAGT GTGTGCAGGT GAAGAATGTG GTCAAGACCT CACCTCAGAC TCTGTCCAAC 
CTCTGCCTCA AGATCAATGT CAAACTTGGT GGCATTAACA ACATCCTAGT CCCACACCAG 
CGCTCTGCCG TTTTTCAACA GCCAGTGATA TTCCTGGGAG CAGATGTTAC ACACCCCCCA 
GCAGGGGATG GGAAAAAACC TTCTATCACA GCAGTGGTAG GCAGTATGGA TGCCCACCCC 
AGCCGATACT GTGCTACTGT GCGGGTACAG CGACCACGCC AAGAGATCAT TGAAGACTTG 
TCCTACATGG TGCGTGAGCT CCTCATCCAG TTCTACAAGT CCACCCGCTT CAAGCCTACC 
CGCATCATCT TCTACCGAGA TGGGGTCCCT GAAGGCCAGC TACCCCAGAT ACTCCACTAT 
GAGCTACTGG CCATTCGTGA TGCCTGCATC AAACTGGAAA AGGACTACCA GCCTGGGATC 
ACTTATATTG TGGTGCAGAA ACGCCATCAC ACCCGCCTTT TCTGTGCTGA CAAGAACGAG 
CGAATTGGGA AGAGTGGTAA CATCCCAGCT GGGACCACAG TGGACACCAA CATCACCCAC 
CCATTTGAGT TTGACTTCTA TCTGTGCAGC CATGCAGGCA TCCAGGGCAC CAGCCGACCA 
TCCCATTACT ATGTTCTTTG GGATGACAAC CGTTTCACAG CGGATGAGCT CCAGATCCTG 
ACATACCAGC TGTGCCACAC TTACGTACGA TGCACACGCT CTGTCTCTAT CCCAGCACCT 
GCCTACTATG CCCGCCTGGT GGCTTTCCGG GCACGATACC ACCTGGTGGA CAAGGAGCAT 
GACAGTGGAG AGGGGAGCCA CATATCGGGG CAAAGCAATG GGCGGGACCC CCAGGCCCTG 
GCCAAAGCCG TGCAGGTTCA TCAGGATACT CTGCGCACCA TGTACTTCGC TTGAAGGCAG 
AACGCTGTTA CCTCACTGGA TAGAAGAAAG CTTTCCAAGC CCCAGGAGCT GTGCCACCCA 
AATCCAGAGG AAGCAAGGAG GAGGGAGGTG GGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001307431.1
CDS	161..2836
Translation	MEAGPSGAAAGAYLPPLQQVFQAPRRPGIGTVGKPIKLLANYFEVDIPKIDVYHYEVDIKPDKCPRRVNREVVEYMVQHFKPQIFGDRKPVYDGKKNIYTVTALPIGNERVDFEVTIPGEGKDRIFKVSIKWLAIVSWRMLHEALVSGQIPVPLESVQALDVAMRHLASMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKPLTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNASYNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYGGRNRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGITYIVVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHISGQSNGRDPQALAKAVQVHQDTLRTMYFA*RQNAVTSLDRRKLSKPQELCHPNPEEARRREVG

Target ORF information:

RefSeq Version	NM_001320502.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta argonaute RISC component 1 (AGO1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001320502.1

ORF Insert Sequence:

ATGGAAGCGG GACCCTCGGG AGCAGCTGCG GGCGCTTACC TGCCCCCCCT GCAGCAGGTG 
TTCCAGGCAC CTCGCCGGCC TGGCATTGGC ACTGTGGGGA AACCAATCAA GCTCCTGGCC 
AATTACTTTG AGGTGGACAT CCCTAAGATC GACGTGTACC ACTACGAGGT GGACATCAAG 
CCGGATAAGT GTCCCCGTAG AGTCAACCGG GAAGTGGTGG AATACATGGT CCAGCATTTC 
AAGCCTCAGA TCTTTGGTGA TCGCAAGCCT GTGTATGATG GAAAGAAGAA CATTTACACT 
GTCACAGCAC TGCCCATTGG CAACGAACGG GTCGACTTTG AGGTGACAAT CCCTGGGGAA 
GGGAAGGATC GAATCTTTAA GGTCTCCATC AAGTGGCTAG CCATTGTGAG CTGGCGAATG 
CTGCATGAGG CCCTGGTCAG CGGCCAGATC CCTGTTCCCT TGGAGTCTGT GCAAGCCCTG 
GATGTGGCCA TGAGGCACCT GGCATCCATG AGGTACACCC CTGTGGGCCG CTCCTTCTTC 
TCACCGCCTG AGGGCTACTA CCACCCGCTG GGGGGTGGGC GCGAGGTCTG GTTCGGCTTT 
CACCAGTCTG TGCGCCCTGC CATGTGGAAG ATGATGCTCA ACATTGATGT CTCAGCCACT 
GCCTTCTATA AGGCGCAGCC AGTGATTGAG TTCATGTGTG AGGTGCTGGA CATCAGGAAC 
ATAGATGAGC AGCCCAAGCC CCTCACGGAC TCTCAGCGTG TTCGCTTCAC CAAGGAGATC 
AAGGGCCTGA AGGTGGAAGT CACCCACTGT GGACAGATGA AGAGGAAATA CCGCGTGTGT 
AATGTTACCC GTCGCCCTGC TAGCCATCAG ACATTCCCCT TACAGCTGGA GAGTGGACAG 
ACTGTGGAGT GCACAGTGGC ACAGTATTTC AAGCAGAAAT ATAACCTTCA GCTCAAGTAT 
CCCCATCTGC CCTGCCTACA AGTTGGCCAG GAACAAAAGC ATACCTACCT TCCCCTAGAG 
GTCTGTAACA TTGTGGCTGG GCAACGCTGT ATTAAAAAGC TGACCGACAA CCAGACTTCG 
ACCATGATAA AGGCCACAGC TAGATCCGCT CCAGACAGAC AGGAGGAGAT CAGTCGCCTG 
ATGAAGAATG CCAGCTACAA CTTAGATCCC TACATCCAGG AATTTGGGAT CAAAGTGAAG 
GATGACATGA CGGAGGTGAC AGGGCGAGTG CTGCCGGCGC CCATCTTGCA GTACGGCGGC 
CGGAACCGGG CCATTGCCAC ACCCAATCAG GGTGTCTGGG ACATGCGGGG GAAACAGTTC 
TACAATGGGA TTGAGATCAA AGTCTGGGCC ATCGCCTGCT TCGCACCCCA AAAACAGTGT 
CGAGAAGAGG TGCTCAAGAA CTTCACAGAC CAGCTGCGGA AGATTTCCAA GGATGCGGGG 
ATGCCTATCC AGGGTCAGCC TTGTTTCTGC AAATATGCCC AGGGGGCAGA CAGCGTGGAG 
CCTATGTTCC GGCATCTCAA GAACACCTAC TCAGGGCTGC AGCTCATTAT TGTCATCCTG 
CCAGGGAAGA CGCCAGTGTA TGCTGAGGTG AAACGTGTTG GAGATACACT CTTGGGAATG 
GCTACACAGT GTGTGCAGGT GAAGAATGTG GTCAAGACCT CACCTCAGAC TCTGTCCAAC 
CTCTGCCTCA AGATCAATGT CAAACTTGGT GGCATTAACA ACATCCTAGT CCCACACCAG 
CGCTCTGCCG TTTTTCAACA GCCAGTGATA TTCCTGGGAG CAGATGTTAC ACACCCCCCA 
GCAGGGGATG GGAAAAAACC TTCTATCACA GCAGTGGTAG GCAGTATGGA TGCCCACCCC 
AGCCGATACT GTGCTACTGT GCGGGTACAG CGACCACGCC AAGAGATCAT TGAAGACTTG 
TCCTACATGG TGCGTGAGCT CCTCATCCAG TTCTACAAGT CCACCCGCTT CAAGCCTACC 
CGCATCATCT TCTACCGAGA TGGGGTCCCT GAAGGCCAGC TACCCCAGAT ACTCCACTAT 
GAGCTACTGG CCATTCGTGA TGCCTGCATC AAACTGGAAA AGGACTACCA GCCTGGGATC 
ACTTATATTG TGGTGCAGAA ACGCCATCAC ACCCGCCTTT TCTGTGCTGA CAAGAACGAG 
CGAATTGGGA AGAGTGGTAA CATCCCAGCT GGGACCACAG TGGACACCAA CATCACCCAC 
CCATTTGAGT TTGACTTCTA TCTGTGCAGC CATGCAGGCA TCCAGGGCAC CAGCCGACCA 
TCCCATTACT ATGTTCTTTG GGATGACAAC CGTTTCACAG CGGATGAGCT CCAGATCCTG 
ACATACCAGC TGTGCCACAC TTACGTACGA TGCACACGCT CTGTCTCTAT CCCAGCACCT 
GCCTACTATG CCCGCCTGGT GGCTTTCCGG GCACGATACC ACCTGGTGGA CAAGGAGCAT 
GACAGTGGAG AGGGGAGCCA CATATCGGGG CAAAGCAATG GGCGGGACCC CCAGGCCCTG 
GCCAAAGCCG TGCAGGTTCA TCAGGATACT CTGCGCACCA TGTACTTCGC TTGAAGGCAG 
AACGCTGTTA CCTCACTGGA TAGAAGAAAG CTTTCCAAGC CCCAGGAGCT GTGCCACCCA 
AATCCAGAGG AAGCAAGGAG GAGGGAGGTG GGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGO1 ( NM_001320501.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> NP_001307430.1 Macaca mulatta argonaute RISC component 1 (AGO1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMb57534
Clone ID Related Accession (Same CDS sequence)	NM_001320501.1
Accession Version	NM_001320501.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2574bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-30
Organism	Macaca mulatta(Rhesus monkey)
Product	protein argonaute-1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from JU475150.1, JV540982.1 and JV578265.1. On Feb 25, 2016 this sequence version replaced XM_015135323.1. Transcript Variant: This variant (1) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon, while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the shorter isoform (1). ##Evidence-Data-START## Transcript exon combination :: JU475150.1, JV703716.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA4688315, SAMEA4688316 [ECO:0000350] ##Evidence-Data-END##

ATGGAAGCGG GACCCTCGGG AGCAGCTGCG GGCGCTTACC TGCCCCCCCT GCAGCAGGTG 
TTCCAGGCAC CTCGCCGGCC TGGCATTGGC ACTGTGGGGA AACCAATCAA GCTCCTGGCC 
AATTACTTTG AGGTGGACAT CCCTAAGATC GACGTGTACC ACTACGAGGT GGACATCAAG 
CCGGATAAGT GTCCCCGTAG AGTCAACCGG GAAGTGGTGG AATACATGGT CCAGCATTTC 
AAGCCTCAGA TCTTTGGTGA TCGCAAGCCT GTGTATGATG GAAAGAAGAA CATTTACACT 
GTCACAGCAC TGCCCATTGG CAACGAACGG GTCGACTTTG AGGTGACAAT CCCTGGGGAA 
GGGAAGGATC GAATCTTTAA GGTCTCCATC AAGTGGCTAG CCATTGTGAG CTGGCGAATG 
CTGCATGAGG CCCTGGTCAG CGGCCAGATC CCTGTTCCCT TGGAGTCTGT GCAAGCCCTG 
GATGTGGCCA TGAGGCACCT GGCATCCATG AGGTACACCC CTGTGGGCCG CTCCTTCTTC 
TCACCGCCTG AGGGCTACTA CCACCCGCTG GGGGGTGGGC GCGAGGTCTG GTTCGGCTTT 
CACCAGTCTG TGCGCCCTGC CATGTGGAAG ATGATGCTCA ACATTGATGT CTCAGCCACT 
GCCTTCTATA AGGCGCAGCC AGTGATTGAG TTCATGTGTG AGGTGCTGGA CATCAGGAAC 
ATAGATGAGC AGCCCAAGCC CCTCACGGAC TCTCAGCGTG TTCGCTTCAC CAAGGAGATC 
AAGGGCCTGA AGGTGGAAGT CACCCACTGT GGACAGATGA AGAGGAAATA CCGCGTGTGT 
AATGTTACCC GTCGCCCTGC TAGCCATCAG ACATTCCCCT TACAGCTGGA GAGTGGACAG 
ACTGTGGAGT GCACAGTGGC ACAGTATTTC AAGCAGAAAT ATAACCTTCA GCTCAAGTAT 
CCCCATCTGC CCTGCCTACA AGTTGGCCAG GAACAAAAGC ATACCTACCT TCCCCTAGAG 
GTCTGTAACA TTGTGGCTGG GCAACGCTGT ATTAAAAAGC TGACCGACAA CCAGACTTCG 
ACCATGATAA AGGCCACAGC TAGATCCGCT CCAGACAGAC AGGAGGAGAT CAGTCGCCTG 
ATGAAGAATG CCAGCTACAA CTTAGATCCC TACATCCAGG AATTTGGGAT CAAAGTGAAG 
GATGACATGA CGGAGGTGAC AGGGCGAGTG CTGCCGGCGC CCATCTTGCA GTACGGCGGC 
CGGAACCGGG CCATTGCCAC ACCCAATCAG GGTGTCTGGG ACATGCGGGG GAAACAGTTC 
TACAATGGGA TTGAGATCAA AGTCTGGGCC ATCGCCTGCT TCGCACCCCA AAAACAGTGT 
CGAGAAGAGG TGCTCAAGAA CTTCACAGAC CAGCTGCGGA AGATTTCCAA GGATGCGGGG 
ATGCCTATCC AGGGTCAGCC TTGTTTCTGC AAATATGCCC AGGGGGCAGA CAGCGTGGAG 
CCTATGTTCC GGCATCTCAA GAACACCTAC TCAGGGCTGC AGCTCATTAT TGTCATCCTG 
CCAGGGAAGA CGCCAGTGTA TGCTGAGGTG AAACGTGTTG GAGATACACT CTTGGGAATG 
GCTACACAGT GTGTGCAGGT GAAGAATGTG GTCAAGACCT CACCTCAGAC TCTGTCCAAC 
CTCTGCCTCA AGATCAATGT CAAACTTGGT GGCATTAACA ACATCCTAGT CCCACACCAG 
CGCTCTGCCG TTTTTCAACA GCCAGTGATA TTCCTGGGAG CAGATGTTAC ACACCCCCCA 
GCAGGGGATG GGAAAAAACC TTCTATCACA GCAGTGGTAG GCAGTATGGA TGCCCACCCC 
AGCCGATACT GTGCTACTGT GCGGGTACAG CGACCACGCC AAGAGATCAT TGAAGACTTG 
TCCTACATGG TGCGTGAGCT CCTCATCCAG TTCTACAAGT CCACCCGCTT CAAGCCTACC 
CGCATCATCT TCTACCGAGA TGGGGTCCCT GAAGGCCAGC TACCCCAGAT ACTCCACTAT 
GAGCTACTGG CCATTCGTGA TGCCTGCATC AAACTGGAAA AGGACTACCA GCCTGGGATC 
ACTTATATTG TGGTGCAGAA ACGCCATCAC ACCCGCCTTT TCTGTGCTGA CAAGAACGAG 
CGAATTGGGA AGAGTGGTAA CATCCCAGCT GGGACCACAG TGGACACCAA CATCACCCAC 
CCATTTGAGT TTGACTTCTA TCTGTGCAGC CATGCAGGCA TCCAGGGCAC CAGCCGACCA 
TCCCATTACT ATGTTCTTTG GGATGACAAC CGTTTCACAG CGGATGAGCT CCAGATCCTG 
ACATACCAGC TGTGCCACAC TTACGTACGA TGCACACGCT CTGTCTCTAT CCCAGCACCT 
GCCTACTATG CCCGCCTGGT GGCTTTCCGG GCACGATACC ACCTGGTGGA CAAGGAGCAT 
GACAGTGGAG AGGGGAGCCA CATATCGGGG CAAAGCAATG GGCGGGACCC CCAGGCCCTG 
GCCAAAGCCG TGCAGGTTCA TCAGGATACT CTGCGCACCA TGTACTTCGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001307430.1
CDS	161..2734
Translation	MEAGPSGAAAGAYLPPLQQVFQAPRRPGIGTVGKPIKLLANYFEVDIPKIDVYHYEVDIKPDKCPRRVNREVVEYMVQHFKPQIFGDRKPVYDGKKNIYTVTALPIGNERVDFEVTIPGEGKDRIFKVSIKWLAIVSWRMLHEALVSGQIPVPLESVQALDVAMRHLASMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKPLTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNASYNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYGGRNRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGITYIVVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHISGQSNGRDPQALAKAVQVHQDTLRTMYFA

Target ORF information:

RefSeq Version	NM_001320501.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta argonaute RISC component 1 (AGO1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001320501.1

ORF Insert Sequence:

ATGGAAGCGG GACCCTCGGG AGCAGCTGCG GGCGCTTACC TGCCCCCCCT GCAGCAGGTG 
TTCCAGGCAC CTCGCCGGCC TGGCATTGGC ACTGTGGGGA AACCAATCAA GCTCCTGGCC 
AATTACTTTG AGGTGGACAT CCCTAAGATC GACGTGTACC ACTACGAGGT GGACATCAAG 
CCGGATAAGT GTCCCCGTAG AGTCAACCGG GAAGTGGTGG AATACATGGT CCAGCATTTC 
AAGCCTCAGA TCTTTGGTGA TCGCAAGCCT GTGTATGATG GAAAGAAGAA CATTTACACT 
GTCACAGCAC TGCCCATTGG CAACGAACGG GTCGACTTTG AGGTGACAAT CCCTGGGGAA 
GGGAAGGATC GAATCTTTAA GGTCTCCATC AAGTGGCTAG CCATTGTGAG CTGGCGAATG 
CTGCATGAGG CCCTGGTCAG CGGCCAGATC CCTGTTCCCT TGGAGTCTGT GCAAGCCCTG 
GATGTGGCCA TGAGGCACCT GGCATCCATG AGGTACACCC CTGTGGGCCG CTCCTTCTTC 
TCACCGCCTG AGGGCTACTA CCACCCGCTG GGGGGTGGGC GCGAGGTCTG GTTCGGCTTT 
CACCAGTCTG TGCGCCCTGC CATGTGGAAG ATGATGCTCA ACATTGATGT CTCAGCCACT 
GCCTTCTATA AGGCGCAGCC AGTGATTGAG TTCATGTGTG AGGTGCTGGA CATCAGGAAC 
ATAGATGAGC AGCCCAAGCC CCTCACGGAC TCTCAGCGTG TTCGCTTCAC CAAGGAGATC 
AAGGGCCTGA AGGTGGAAGT CACCCACTGT GGACAGATGA AGAGGAAATA CCGCGTGTGT 
AATGTTACCC GTCGCCCTGC TAGCCATCAG ACATTCCCCT TACAGCTGGA GAGTGGACAG 
ACTGTGGAGT GCACAGTGGC ACAGTATTTC AAGCAGAAAT ATAACCTTCA GCTCAAGTAT 
CCCCATCTGC CCTGCCTACA AGTTGGCCAG GAACAAAAGC ATACCTACCT TCCCCTAGAG 
GTCTGTAACA TTGTGGCTGG GCAACGCTGT ATTAAAAAGC TGACCGACAA CCAGACTTCG 
ACCATGATAA AGGCCACAGC TAGATCCGCT CCAGACAGAC AGGAGGAGAT CAGTCGCCTG 
ATGAAGAATG CCAGCTACAA CTTAGATCCC TACATCCAGG AATTTGGGAT CAAAGTGAAG 
GATGACATGA CGGAGGTGAC AGGGCGAGTG CTGCCGGCGC CCATCTTGCA GTACGGCGGC 
CGGAACCGGG CCATTGCCAC ACCCAATCAG GGTGTCTGGG ACATGCGGGG GAAACAGTTC 
TACAATGGGA TTGAGATCAA AGTCTGGGCC ATCGCCTGCT TCGCACCCCA AAAACAGTGT 
CGAGAAGAGG TGCTCAAGAA CTTCACAGAC CAGCTGCGGA AGATTTCCAA GGATGCGGGG 
ATGCCTATCC AGGGTCAGCC TTGTTTCTGC AAATATGCCC AGGGGGCAGA CAGCGTGGAG 
CCTATGTTCC GGCATCTCAA GAACACCTAC TCAGGGCTGC AGCTCATTAT TGTCATCCTG 
CCAGGGAAGA CGCCAGTGTA TGCTGAGGTG AAACGTGTTG GAGATACACT CTTGGGAATG 
GCTACACAGT GTGTGCAGGT GAAGAATGTG GTCAAGACCT CACCTCAGAC TCTGTCCAAC 
CTCTGCCTCA AGATCAATGT CAAACTTGGT GGCATTAACA ACATCCTAGT CCCACACCAG 
CGCTCTGCCG TTTTTCAACA GCCAGTGATA TTCCTGGGAG CAGATGTTAC ACACCCCCCA 
GCAGGGGATG GGAAAAAACC TTCTATCACA GCAGTGGTAG GCAGTATGGA TGCCCACCCC 
AGCCGATACT GTGCTACTGT GCGGGTACAG CGACCACGCC AAGAGATCAT TGAAGACTTG 
TCCTACATGG TGCGTGAGCT CCTCATCCAG TTCTACAAGT CCACCCGCTT CAAGCCTACC 
CGCATCATCT TCTACCGAGA TGGGGTCCCT GAAGGCCAGC TACCCCAGAT ACTCCACTAT 
GAGCTACTGG CCATTCGTGA TGCCTGCATC AAACTGGAAA AGGACTACCA GCCTGGGATC 
ACTTATATTG TGGTGCAGAA ACGCCATCAC ACCCGCCTTT TCTGTGCTGA CAAGAACGAG 
CGAATTGGGA AGAGTGGTAA CATCCCAGCT GGGACCACAG TGGACACCAA CATCACCCAC 
CCATTTGAGT TTGACTTCTA TCTGTGCAGC CATGCAGGCA TCCAGGGCAC CAGCCGACCA 
TCCCATTACT ATGTTCTTTG GGATGACAAC CGTTTCACAG CGGATGAGCT CCAGATCCTG 
ACATACCAGC TGTGCCACAC TTACGTACGA TGCACACGCT CTGTCTCTAT CCCAGCACCT 
GCCTACTATG CCCGCCTGGT GGCTTTCCGG GCACGATACC ACCTGGTGGA CAAGGAGCAT 
GACAGTGGAG AGGGGAGCCA CATATCGGGG CAAAGCAATG GGCGGGACCC CCAGGCCCTG 
GCCAAAGCCG TGCAGGTTCA TCAGGATACT CTGCGCACCA TGTACTTCGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A new rhesus macaque assembly and annotation for next-generation sequencing analyses.
Biology direct9(1)20(2014 Oct)
Zimin AV,Cornish AS,Maudhoo MD,Gibbs RM,Zhang X,Pandey S,Meehan DT,Wipfler K,Bosinger SE,Johnson ZP,Tharp GK,Mar?ais G,Roberts M,Ferguson B,Fox HS,Treangen T,Salzberg SL,Yorke JA,Norgren RB Jr

Programmed translational readthrough generates antiangiogenic VEGF-Ax.
Cell157(7)1605-18(2014 Jun)
Eswarappa SM,Potdar AA,Koch WJ,Fan Y,Vasu K,Lindner D,Willard B,Graham LM,DiCorleto PE,Fox PL

Misreading of termination codons in eukaryotes by natural nonsense suppressor tRNAs.
Nucleic acids research29(23)4767-82(2001 Dec)
Beier H,Grimm M

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AGO1 cDNA ORF clone, Macaca mulatta(Rhesus monkey)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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