SPTB cDNA ORF clone, Gallus gallus(chicken)

The following SPTB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPTB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa151188 XM_015286286.2
Latest version!
Gallus gallus spectrin beta, erythrocytic (SPTB), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OGa151189 XM_015286287.2
Latest version!
Gallus gallus spectrin beta, erythrocytic (SPTB), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OGa36099 XM_015286286.1
Latest version!
Gallus gallus spectrin, beta, erythrocytic (SPTB), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OGa36100 XM_015286287.1
Latest version!
Gallus gallus spectrin, beta, erythrocytic (SPTB), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa151188
    Clone ID Related Accession (Same CDS sequence) XM_015286286.2
    Accession Version XM_015286286.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6891bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product spectrin beta chain, erythrocytic isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015286286.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGACCTCCG AGAATGACTA CGACCATCTG GAGCTGCAGC AGTCCTACAG CCACTGGGAT 
    GCCTCCGACG ATGAGTTGGA CAACGACAAC AGCTCAGCGC GGCTCTTTGA GCGCTCCCGC
    ATCAAAGCTC TGGCAGATGA GCGGGAGGCG GTGCAGAAGA AGACCTTCAC CAAGTGGGTG
    AATCTGCACT TGGCGTGCGT CACCTGCCGC ATCTCCGACC TCTACCTGGA CCTCCGGGAC
    GGGCGGATGC TCATCAAGCT GCTGGAGGTG CTGTCCAAAG AGATGCTGCC CAAGCCCACC
    AAGGGCCGGA TGCGCATCCA CTGCCTGGAG AACGTGGACA AGGCACTGCA GTTCCTGAAG
    GAGAAGCAAG TGCACCTGGA GAACATGGGC TCCCACGACA TCGTGGATGG CAACCACCGC
    CTCATCCTCG GCCTCATCTG GACCATCATC CTCCGCTTCC AGGTGCAAGG CGTCATCGAG
    GATATGGAGG AGGATACAGA GACACGCTCG CCCAGGGAAG CGCTGCTGCT CTGGTGCCAA
    ATGAAGACGA AAGGCTACCC TCACGTGAAT GTCACCAACT TCACCTCCAG CTGGAAGGAT
    GGACTGGCCT TCAACGCCCT CATCCACAAG CACAGGCCTG AGCTGTTTGA CTTCAAAACT
    CTGACCAAGT CCAACGCCCG GCACAACCTG GAGCATGCCT TCAGCATGGC CGAGCGGCAC
    CTGGGCATCA CCCCGCTCCT CGACCCTGAA GATGTGTTCA CGGAGAACCC CGATGAGAAG
    TCCATCATCA CCTACGTGGT GGCCTTCTAC CACTACTTCT CCGAGATGAA GAAACTGGAG
    GTGAAGGGCA GGCGGCTGGG CAAGGTCATT GAGCACGCCA AAGAGACCAA GCGGATGATC
    GAGGGCTACG GGGGCCTGGC CTCCAACCTG CTCACCTGGA TCGAGCAGAC CATCATCTCC
    CTCAACAGCC GCAGCTTTGC CAACTCGCTG GCCGGTGTGC AGCACCAGCT GCAGACCTTC
    AGCACCTACC GCACGGTGGA GAAGCCCCCC AAGTTTCAGG AGAAGGGCAA CCTGGAGGTG
    CTGCTCTTCA CCATCCAGTC TCGGATGCGA GCCAACAACC AGCGTGTCTA CACACCACAA
    GAGGGGCGTC TGGTCTGTGA CATCAACCGG GCATGGGAGA AGCTGGAGAA AGCAGAGCAT
    GAGCGGGAGC TGGCACTGCG CAATGAGCTC ATCCGGCAGG AGAAGCTGGA GCAGCTGGCA
    CGGCGCTTTG ACCGCAAAGC AGCCATGCGG GAGGCCTGGC TGAGTGAGAA CCAACGCCTG
    GTGGCACAGC AGGATAACTT TGGCCACGAC CTGACGGCGG TGGAGGCGGC CAAGAAGAAA
    CACGAGGCCA TTGAGACGGA CACAGCTGCC TACATGGAAC GGGTGCAGGC CATTGATGCA
    GTGGCGAAGG AGCTGGAGAG GGAGGGCTAC CACGACATCA AACGCATCAG GGGGCGCAAG
    GACAACATCC TGCAGCACTG GGAGTACCTC CAGGAGCTGC TGCGCAACCG GCGCCAGCGC
    CTTGAGATGA ACCTCACCCT GCAGCACCTC TTCCAGGAGA TGCTGCACAG CATCAACTGG
    ATGGATGAGG TCAAGGTGCA GCTGGCTTCA TCCAAATCTG GGAAGCACCT CCTTGAGGTG
    GAGGAGCTTC TGGAGACCCA CCGGCTGCTG GAAAGTGACA TGGCCCTGCA GGCAGAGAAG
    GTGCGGGCCA TCAGCACTGC TGCCCTCCGA TTTGCCGACG CTGAGGGCTA CCGCCCCTGC
    GACCCCAGAG TGATCCGAGA CCGCGTGAAC CACCTGGAGA TGTGCCGGCG GGAGCTGCAG
    GCACTGGCGG CGAGGCGTAA AGCCCTGCTG GAGCAGTCCC GGGCTGTCTG GCAGTGTCTG
    CAGGAGCTGG ACGAGGCAGA GAGCTGGATC AAGGAGCAGG AGCAGATCTA CTCAGCGCTG
    GACTACGGCA AAGACCTGGC GGGCGTGCTG CTGCTGCGAC GCCGTCACAC CGCGCTGGAG
    GCTGAGCTGG AGGCACGGGG AGCCCGGCTG GACAAGGCGC TGGTGATGGC TGAGCAGCTG
    GCAGCAGCAG GGCGTGAGGC CGGGCGGCTG CGGGACCGGG CGGCTGCCGT GCGGGTGCTG
    TGGGACCAGC TGCAGGAGCT GGTGGCCTTC CGCCGCCGCG GGCTGCGGGA TGCCGAGGGC
    TTCTTCCAGT TCCAGGCAGA GGCTGAAGAG CTGTCAGAGG TTCTTACGGA GGCTCAGCAG
    CGGGCGGCCA GCGAGGAGCT GGGTCACGAC GAGGTCCACA CGCAGGCACT GCTGCGGGAG
    CACCAGGAGC TGCTGGAGGA GCTGGCGGCT GCCCAGCAGC TCCTGGAGCG CTTGGGTCAC
    CAAGCAGAGG GCTTCCCACC AGAGCTGCGG GCCGGACCCG AGGCGCACAA CCGGCTGGCA
    GCGCTGCGGG AGCTGCACCA CAAGGTGAGC ACGCTGGCCG AGACGCGTGG CCGAAGGCTG
    CGGGATGCCC TCAACCTCTA CACTGTTTTT GGAGAGAGCG AAGCCTGCCA GCTCTGGATG
    GGCGCCAAGG AGCGGTGGCT GGAGAAGCTG GAGGTGCCCA ACAAGCTGGA GGACCTGGAC
    GTGGTGAAGC ACAGGCTGGA TGGGCTGGAG CAGGAGATGG TTGGCGTGGC TTCTCAAATC
    GACGCTGTCA ACCGCTCAGC TGACGGTCTG CTGGAGAGCG GCCACCCACG CAGCCCACAG
    GTCCGGCAGT GCCAGCAGCA GCTCAATGAG CGGTGGGACC GTTTTCGGGA GCTGGTGTCC
    CAGTGGCGTT CAGCAGTGGG CTCGGCGCTC AACCTGCTCA GCTTCCAACT GGAGTGCGAG
    GAGACGCGCG CCTGGCTGCT GAGCAAGACA CGGGTGGTGG AGTCCACAAA GGAGCTCGGC
    CGTGATCTGG CTGGAGTCCT GGCCACCCAG CGCAAGCTGT ATGGCATTGA ACGTGAGCTG
    GCAGCAACCG AGAGCCGCCT GGGTGCCCTG CGCCCGCAAG CCGCCCTCCT GGCCCAGGAG
    CGCCCTGAGT TGGCCGAGGA CACGGCACGG CGTCTGGCGG GGGCACAGGA TGCCATGGAC
    GGGCTGCAGG GCGCCCTGCG GGACCGAGCG GCTGCGCTGG GAGAAGTGGG GCAGCTGCAG
    AGCTTCCTGC AGGACCTGGA TGACTTCCAA GCGTGGCTCT TTGGGGCGCA GAAAGCCGTG
    GCAGGCACCG AAGAGGTGCC GACTTCGGTG GGTGAGGCAG AAGAGCTGCT GCGGAAGCAT
    GCGGCTGCCC TCGAGGATGC CGAGGGGCAC ACAGCTGCCT TCACGGCGCT GCTGGAGGCA
    GGCGAGCGGG TGACAAGCAA CCAGGAGGAC CCCGAGTATG AGCAGCTGCG GGAGAGGCTG
    CGGGGCGTGG AGGCGGGCTG GGGAGCCCTG CACAAGATGT GGGATGCCCG GCAGCGCTTC
    CTTGCCCAGT GCTTGGGATT CCAGGAGTTC CTGCGTGACG CCAAACAGGC TGAGATTCTC
    CTCGCCAACC AGGAGTACAC GCTGGCCCAC CTGGAGCTAC CCCCTACACT GGAGGGTTCC
    ACAGCTGCCC TGCGCCGCTT TGAGGATTTC CGTGCCTCCA TGGAGAGCAG CGCTGAGAAG
    GTCCCTAGCG TGGTGGCCGC TGGCTCCAAG CTTGTGGCAG AGGGCAACAT CTTCTCTGAG
    AAGATCAGTG AGAAGAACCA GGCCCTGCAG GAGCGGCACC AGGTGAATGT GGCCAAGGCA
    CAGGAGGCGG CGGGCTTGCT GCAGGACAAC CACAGGCTGC AGAGCTTCCT GCAGAGCTGC
    CGGGAGCTCG CTGCTTGGGT GGATGAGAAG ATGCTGACAG TGCAGGACGC GTCCTATGGA
    GGTGCACGTG GTTTGCATGG CAAGTGGCAG AAGCACCAAG CCTTTATGGC AGAGCTGGCA
    GCCAACGAGG CATGGCTGGA GAAGATCAAA GCGGAGGGCA TGGAGCTGGC GAGCTGCAAA
    CCACAGTACG GTGCAGTGGT GGGCCAGCGG CTGGACGAGC TGCAGGCGCT GTGGAATGGA
    CTGCACGACG CTGCCAAGGA GAAGGGCCAG CAGCTCTTTG AGGCCAACCG CACTGAGCTG
    TACGCCCAGA GCTACAGTGA CCTGGAGCGC TGGCTGGGGC AGGTGGAGGG CGAGCTGCAC
    ACCACTGAGC GTGCCAAGGA CCTCACCAGC GCCAACCTAC TGCTCAAGAA ACTGACGCGG
    CTGGAGGAGC AGGTGGCGGC ACGACAGAAG GAACTGATGG AGCTGAAAGC ACCACTTGCT
    GGGGTCGCAC CAGAGCCCGA TGCGCAGGAG GAGAAGCTCC AGCAGCGATT CCTTGACCTG
    CTGGAGCCAC TGAGGAAGAA GAGGAAGGAG CTGGAAACCT CCAAGGCGAT GTACCAGCTG
    GGGAGGGACC TGGAGGACGA GATGTTATGG GTGCAGGAGA GGATGCCATG GGCCAGGTCA
    ACTGAGAACG GCACCAACCT CCAGACCGTG CAGCGTTTGG CCAAGAGGAA TGAGACCCTA
    CAGAAGGAGC TGCAGGGCCA TGCCCCCCGC CTGGCCGAGG TGCTGGAACG TGGGGAGGCG
    GCTGCTGGGG GACCCTGCCC GGAGCTGGCG GAGAGGGCAC GGGAGCTGCA GGCGCAGTGG
    GAAGCGCTGA GGGAAGAAGT GGTCGCACGG CAGCAGCGGC TGCGGGAGGC CAGCGAGGCC
    CAGCAGTACT ACCTGGATGC TGATGAGGCC AAAGCGTGGG TCAGCGAGCA GGAGCTCTTC
    ATGGGAGATG AGGAGAAGCC GAAGGACGAG GAAAGTGGTT TGGTGATGCT GAAGAGACAC
    GTGAGGCAGC AGCGCTCCAT CGAGGACTAT GGGCAGACCA TCAAGGAGCT GGCGGGGAGG
    GCTCAGCAGC TGCTCTCTGC TGGCCACCCT GAGGGGGAGC AGATCATCCG GCTGCAGGGC
    CAGGTGGACA AGTATTACGC GGGGCTGAAG GAGGCGGCCG AGGAGCGCCG GCGGCGCTTG
    GAGAACATGT GCCACCTCTT CCAGCTGAAG CGTGAGGTGG AGGACCTGGA GCAGTGGATT
    GCTGAGCGTG ACGTGGCAGC CTCCTCCCAG GAGCTGGGGC AGGACCTGGA CCATGTCACG
    ATACTGAGGG ATAAGTTTCG GGAGTTTGCA CGGGAAACAG GCAGCGTAGG GCAGGAACGT
    GTGGACCACG TCAACCTGAC CATCGAGGAC CTCATCGACG CGGGGCACGC CGAGGCTGCC
    ACCATGGCTG AGTGGAAGGA CGGACTGAAC GAGAGCTGGG CTGACCTCCT GGAGCTGATT
    GACACACGCA TGCAGTTGCT GGCCGCCTCC TATGATCTGC GCAAGTACTT CTATGACGGC
    TCTGAGCTGC TGGCCCTCAT TGCTGCCCGC CACCAGGAGC TGCCCCAGGA CCTGGGCGAC
    GACTCGGGTT CGGTGGAGGC TTTCCACCGC ATGCACAGCG CCTTCGAGCG TGACCTGCAG
    CTGCTGGAGG GGCAGGTGCA GCGGTTTCGA GAGGTGGCAG CTCGCCTGCA AACCGCCTAT
    GCTGGGGAGA AGGCAGTCAG CATCCAGCAG CAGGAGCAGG AGGTGGCACG GGCGCTGCAG
    GCACTGCTGG AGGCATGCAG TGGGCGCCGG GCGCAGCTGG TGGACACAGC CGACAAGTTC
    CGCTTCTTCA GCATGGCCCG TGACCTGCTC TCCTGGATGG AGAGCACTGT GCGGCAGATC
    CAGACACAGG AGAAGCCCAG GGACGTTTCC TCGGTGGAGC TGCTGATGAA GTACCACCAA
    GGCATCCACG CCGAGATAGA TGCCCGCGAC AAGAGCTTTG ATGCCTGCAT TGAACTGGGC
    AAGAAGCTGC TGCAGCGCAA ACACCGGGAG TCTCCTGAGA TCAAGGCAAA GCTGATGGAG
    CTGCTGGACA AGAGGAAGGC CATGATGGAG ATGTGGCAGA AGCGCTGGGA CCGGCTGCGG
    CTGCTGTTGG AGGTGTGCCA GTTCTCCCGG GACGCCTCAG TGGCCGAGTC GTGGCTGATG
    GCACAGGAGC CCTACCTGGC CAGCAGCGAC TACGGGCAGA CGGTGGACGC GGTGGAGAAG
    TTGCTGAAAC GGCACGAAGC CTTTGAGAAG TCCACGGCCA CGTGGGAGGA GCGCATCGCT
    GCCCTGAGGA AGCTGACGAC GCTGGAGCTC ATGGGCGGGC GGACGCTGCG GGAGGAGGAC
    GTGGTGCACG ACGCTGCTCC CGAACGTCAG CTGGATCTGG ATGCAGAGCT GGAGGCAGGG
    TCTGAGGAGG AGGAGGAGAA GAGGAAGGGG GTGATCCCTC AGGAGGCCTC TCCGCCCGCT
    GTCGATGGAC CCCAGCCGCT GACAGTGCCC GAGGAGGAGC CCGTAGCACC GCGGGAGGAG
    GTGGCCACAT TGCCCGCCCA GCCTGCCCAC GTTCAGCTGG AGGGCTACCT GGGCCGCAAG
    CACGACCTGG AGGCGGCCAC CAAGCGTGCG TCCAACAGGT CGTGGAGCAC GCGGTACTGC
    GTGCTGCGGG GCGAGGAGCT TGCCTTCTTC AAGGACGCCA AGAGCCGGGC ACTGGGGGTG
    CCCTGCCAGG GTGAGGAGCC CCTGGGGCTG CGCAACGCAC GCTGCCAGGT GGCTGCTGGC
    TACAAGAAGA AGAAGCACGT CTTTGCGCTC AGGCTCAGCA ATGGCAGTGA GTGGCTCTTC
    CACGGCAAGG ATGAGGAGGA GATGCAGACG TGGCTGCAGG GGCTGCACGT GGCCATCACC
    TCCTGTCAGA ACCTGCCGGC CAAGGCGCGC AGCCTGCCCC CGCCCCACGC CCCCCCCGAG
    CCCCGGAGGG ACAAGGAGAA GCGCTTCAGC TTCTTCCCCA AGAAGAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015141772.2
    CDS193..7083
    Translation

    Target ORF information:

    RefSeq Version XM_015286286.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spectrin beta, erythrocytic (SPTB), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015286286.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGACCTCCG AGAATGACTA CGACCATCTG GAGCTGCAGC AGTCCTACAG CCACTGGGAT 
    GCCTCCGACG ATGAGTTGGA CAACGACAAC AGCTCAGCGC GGCTCTTTGA GCGCTCCCGC
    ATCAAAGCTC TGGCAGATGA GCGGGAGGCG GTGCAGAAGA AGACCTTCAC CAAGTGGGTG
    AATCTGCACT TGGCGTGCGT CACCTGCCGC ATCTCCGACC TCTACCTGGA CCTCCGGGAC
    GGGCGGATGC TCATCAAGCT GCTGGAGGTG CTGTCCAAAG AGATGCTGCC CAAGCCCACC
    AAGGGCCGGA TGCGCATCCA CTGCCTGGAG AACGTGGACA AGGCACTGCA GTTCCTGAAG
    GAGAAGCAAG TGCACCTGGA GAACATGGGC TCCCACGACA TCGTGGATGG CAACCACCGC
    CTCATCCTCG GCCTCATCTG GACCATCATC CTCCGCTTCC AGGTGCAAGG CGTCATCGAG
    GATATGGAGG AGGATACAGA GACACGCTCG CCCAGGGAAG CGCTGCTGCT CTGGTGCCAA
    ATGAAGACGA AAGGCTACCC TCACGTGAAT GTCACCAACT TCACCTCCAG CTGGAAGGAT
    GGACTGGCCT TCAACGCCCT CATCCACAAG CACAGGCCTG AGCTGTTTGA CTTCAAAACT
    CTGACCAAGT CCAACGCCCG GCACAACCTG GAGCATGCCT TCAGCATGGC CGAGCGGCAC
    CTGGGCATCA CCCCGCTCCT CGACCCTGAA GATGTGTTCA CGGAGAACCC CGATGAGAAG
    TCCATCATCA CCTACGTGGT GGCCTTCTAC CACTACTTCT CCGAGATGAA GAAACTGGAG
    GTGAAGGGCA GGCGGCTGGG CAAGGTCATT GAGCACGCCA AAGAGACCAA GCGGATGATC
    GAGGGCTACG GGGGCCTGGC CTCCAACCTG CTCACCTGGA TCGAGCAGAC CATCATCTCC
    CTCAACAGCC GCAGCTTTGC CAACTCGCTG GCCGGTGTGC AGCACCAGCT GCAGACCTTC
    AGCACCTACC GCACGGTGGA GAAGCCCCCC AAGTTTCAGG AGAAGGGCAA CCTGGAGGTG
    CTGCTCTTCA CCATCCAGTC TCGGATGCGA GCCAACAACC AGCGTGTCTA CACACCACAA
    GAGGGGCGTC TGGTCTGTGA CATCAACCGG GCATGGGAGA AGCTGGAGAA AGCAGAGCAT
    GAGCGGGAGC TGGCACTGCG CAATGAGCTC ATCCGGCAGG AGAAGCTGGA GCAGCTGGCA
    CGGCGCTTTG ACCGCAAAGC AGCCATGCGG GAGGCCTGGC TGAGTGAGAA CCAACGCCTG
    GTGGCACAGC AGGATAACTT TGGCCACGAC CTGACGGCGG TGGAGGCGGC CAAGAAGAAA
    CACGAGGCCA TTGAGACGGA CACAGCTGCC TACATGGAAC GGGTGCAGGC CATTGATGCA
    GTGGCGAAGG AGCTGGAGAG GGAGGGCTAC CACGACATCA AACGCATCAG GGGGCGCAAG
    GACAACATCC TGCAGCACTG GGAGTACCTC CAGGAGCTGC TGCGCAACCG GCGCCAGCGC
    CTTGAGATGA ACCTCACCCT GCAGCACCTC TTCCAGGAGA TGCTGCACAG CATCAACTGG
    ATGGATGAGG TCAAGGTGCA GCTGGCTTCA TCCAAATCTG GGAAGCACCT CCTTGAGGTG
    GAGGAGCTTC TGGAGACCCA CCGGCTGCTG GAAAGTGACA TGGCCCTGCA GGCAGAGAAG
    GTGCGGGCCA TCAGCACTGC TGCCCTCCGA TTTGCCGACG CTGAGGGCTA CCGCCCCTGC
    GACCCCAGAG TGATCCGAGA CCGCGTGAAC CACCTGGAGA TGTGCCGGCG GGAGCTGCAG
    GCACTGGCGG CGAGGCGTAA AGCCCTGCTG GAGCAGTCCC GGGCTGTCTG GCAGTGTCTG
    CAGGAGCTGG ACGAGGCAGA GAGCTGGATC AAGGAGCAGG AGCAGATCTA CTCAGCGCTG
    GACTACGGCA AAGACCTGGC GGGCGTGCTG CTGCTGCGAC GCCGTCACAC CGCGCTGGAG
    GCTGAGCTGG AGGCACGGGG AGCCCGGCTG GACAAGGCGC TGGTGATGGC TGAGCAGCTG
    GCAGCAGCAG GGCGTGAGGC CGGGCGGCTG CGGGACCGGG CGGCTGCCGT GCGGGTGCTG
    TGGGACCAGC TGCAGGAGCT GGTGGCCTTC CGCCGCCGCG GGCTGCGGGA TGCCGAGGGC
    TTCTTCCAGT TCCAGGCAGA GGCTGAAGAG CTGTCAGAGG TTCTTACGGA GGCTCAGCAG
    CGGGCGGCCA GCGAGGAGCT GGGTCACGAC GAGGTCCACA CGCAGGCACT GCTGCGGGAG
    CACCAGGAGC TGCTGGAGGA GCTGGCGGCT GCCCAGCAGC TCCTGGAGCG CTTGGGTCAC
    CAAGCAGAGG GCTTCCCACC AGAGCTGCGG GCCGGACCCG AGGCGCACAA CCGGCTGGCA
    GCGCTGCGGG AGCTGCACCA CAAGGTGAGC ACGCTGGCCG AGACGCGTGG CCGAAGGCTG
    CGGGATGCCC TCAACCTCTA CACTGTTTTT GGAGAGAGCG AAGCCTGCCA GCTCTGGATG
    GGCGCCAAGG AGCGGTGGCT GGAGAAGCTG GAGGTGCCCA ACAAGCTGGA GGACCTGGAC
    GTGGTGAAGC ACAGGCTGGA TGGGCTGGAG CAGGAGATGG TTGGCGTGGC TTCTCAAATC
    GACGCTGTCA ACCGCTCAGC TGACGGTCTG CTGGAGAGCG GCCACCCACG CAGCCCACAG
    GTCCGGCAGT GCCAGCAGCA GCTCAATGAG CGGTGGGACC GTTTTCGGGA GCTGGTGTCC
    CAGTGGCGTT CAGCAGTGGG CTCGGCGCTC AACCTGCTCA GCTTCCAACT GGAGTGCGAG
    GAGACGCGCG CCTGGCTGCT GAGCAAGACA CGGGTGGTGG AGTCCACAAA GGAGCTCGGC
    CGTGATCTGG CTGGAGTCCT GGCCACCCAG CGCAAGCTGT ATGGCATTGA ACGTGAGCTG
    GCAGCAACCG AGAGCCGCCT GGGTGCCCTG CGCCCGCAAG CCGCCCTCCT GGCCCAGGAG
    CGCCCTGAGT TGGCCGAGGA CACGGCACGG CGTCTGGCGG GGGCACAGGA TGCCATGGAC
    GGGCTGCAGG GCGCCCTGCG GGACCGAGCG GCTGCGCTGG GAGAAGTGGG GCAGCTGCAG
    AGCTTCCTGC AGGACCTGGA TGACTTCCAA GCGTGGCTCT TTGGGGCGCA GAAAGCCGTG
    GCAGGCACCG AAGAGGTGCC GACTTCGGTG GGTGAGGCAG AAGAGCTGCT GCGGAAGCAT
    GCGGCTGCCC TCGAGGATGC CGAGGGGCAC ACAGCTGCCT TCACGGCGCT GCTGGAGGCA
    GGCGAGCGGG TGACAAGCAA CCAGGAGGAC CCCGAGTATG AGCAGCTGCG GGAGAGGCTG
    CGGGGCGTGG AGGCGGGCTG GGGAGCCCTG CACAAGATGT GGGATGCCCG GCAGCGCTTC
    CTTGCCCAGT GCTTGGGATT CCAGGAGTTC CTGCGTGACG CCAAACAGGC TGAGATTCTC
    CTCGCCAACC AGGAGTACAC GCTGGCCCAC CTGGAGCTAC CCCCTACACT GGAGGGTTCC
    ACAGCTGCCC TGCGCCGCTT TGAGGATTTC CGTGCCTCCA TGGAGAGCAG CGCTGAGAAG
    GTCCCTAGCG TGGTGGCCGC TGGCTCCAAG CTTGTGGCAG AGGGCAACAT CTTCTCTGAG
    AAGATCAGTG AGAAGAACCA GGCCCTGCAG GAGCGGCACC AGGTGAATGT GGCCAAGGCA
    CAGGAGGCGG CGGGCTTGCT GCAGGACAAC CACAGGCTGC AGAGCTTCCT GCAGAGCTGC
    CGGGAGCTCG CTGCTTGGGT GGATGAGAAG ATGCTGACAG TGCAGGACGC GTCCTATGGA
    GGTGCACGTG GTTTGCATGG CAAGTGGCAG AAGCACCAAG CCTTTATGGC AGAGCTGGCA
    GCCAACGAGG CATGGCTGGA GAAGATCAAA GCGGAGGGCA TGGAGCTGGC GAGCTGCAAA
    CCACAGTACG GTGCAGTGGT GGGCCAGCGG CTGGACGAGC TGCAGGCGCT GTGGAATGGA
    CTGCACGACG CTGCCAAGGA GAAGGGCCAG CAGCTCTTTG AGGCCAACCG CACTGAGCTG
    TACGCCCAGA GCTACAGTGA CCTGGAGCGC TGGCTGGGGC AGGTGGAGGG CGAGCTGCAC
    ACCACTGAGC GTGCCAAGGA CCTCACCAGC GCCAACCTAC TGCTCAAGAA ACTGACGCGG
    CTGGAGGAGC AGGTGGCGGC ACGACAGAAG GAACTGATGG AGCTGAAAGC ACCACTTGCT
    GGGGTCGCAC CAGAGCCCGA TGCGCAGGAG GAGAAGCTCC AGCAGCGATT CCTTGACCTG
    CTGGAGCCAC TGAGGAAGAA GAGGAAGGAG CTGGAAACCT CCAAGGCGAT GTACCAGCTG
    GGGAGGGACC TGGAGGACGA GATGTTATGG GTGCAGGAGA GGATGCCATG GGCCAGGTCA
    ACTGAGAACG GCACCAACCT CCAGACCGTG CAGCGTTTGG CCAAGAGGAA TGAGACCCTA
    CAGAAGGAGC TGCAGGGCCA TGCCCCCCGC CTGGCCGAGG TGCTGGAACG TGGGGAGGCG
    GCTGCTGGGG GACCCTGCCC GGAGCTGGCG GAGAGGGCAC GGGAGCTGCA GGCGCAGTGG
    GAAGCGCTGA GGGAAGAAGT GGTCGCACGG CAGCAGCGGC TGCGGGAGGC CAGCGAGGCC
    CAGCAGTACT ACCTGGATGC TGATGAGGCC AAAGCGTGGG TCAGCGAGCA GGAGCTCTTC
    ATGGGAGATG AGGAGAAGCC GAAGGACGAG GAAAGTGGTT TGGTGATGCT GAAGAGACAC
    GTGAGGCAGC AGCGCTCCAT CGAGGACTAT GGGCAGACCA TCAAGGAGCT GGCGGGGAGG
    GCTCAGCAGC TGCTCTCTGC TGGCCACCCT GAGGGGGAGC AGATCATCCG GCTGCAGGGC
    CAGGTGGACA AGTATTACGC GGGGCTGAAG GAGGCGGCCG AGGAGCGCCG GCGGCGCTTG
    GAGAACATGT GCCACCTCTT CCAGCTGAAG CGTGAGGTGG AGGACCTGGA GCAGTGGATT
    GCTGAGCGTG ACGTGGCAGC CTCCTCCCAG GAGCTGGGGC AGGACCTGGA CCATGTCACG
    ATACTGAGGG ATAAGTTTCG GGAGTTTGCA CGGGAAACAG GCAGCGTAGG GCAGGAACGT
    GTGGACCACG TCAACCTGAC CATCGAGGAC CTCATCGACG CGGGGCACGC CGAGGCTGCC
    ACCATGGCTG AGTGGAAGGA CGGACTGAAC GAGAGCTGGG CTGACCTCCT GGAGCTGATT
    GACACACGCA TGCAGTTGCT GGCCGCCTCC TATGATCTGC GCAAGTACTT CTATGACGGC
    TCTGAGCTGC TGGCCCTCAT TGCTGCCCGC CACCAGGAGC TGCCCCAGGA CCTGGGCGAC
    GACTCGGGTT CGGTGGAGGC TTTCCACCGC ATGCACAGCG CCTTCGAGCG TGACCTGCAG
    CTGCTGGAGG GGCAGGTGCA GCGGTTTCGA GAGGTGGCAG CTCGCCTGCA AACCGCCTAT
    GCTGGGGAGA AGGCAGTCAG CATCCAGCAG CAGGAGCAGG AGGTGGCACG GGCGCTGCAG
    GCACTGCTGG AGGCATGCAG TGGGCGCCGG GCGCAGCTGG TGGACACAGC CGACAAGTTC
    CGCTTCTTCA GCATGGCCCG TGACCTGCTC TCCTGGATGG AGAGCACTGT GCGGCAGATC
    CAGACACAGG AGAAGCCCAG GGACGTTTCC TCGGTGGAGC TGCTGATGAA GTACCACCAA
    GGCATCCACG CCGAGATAGA TGCCCGCGAC AAGAGCTTTG ATGCCTGCAT TGAACTGGGC
    AAGAAGCTGC TGCAGCGCAA ACACCGGGAG TCTCCTGAGA TCAAGGCAAA GCTGATGGAG
    CTGCTGGACA AGAGGAAGGC CATGATGGAG ATGTGGCAGA AGCGCTGGGA CCGGCTGCGG
    CTGCTGTTGG AGGTGTGCCA GTTCTCCCGG GACGCCTCAG TGGCCGAGTC GTGGCTGATG
    GCACAGGAGC CCTACCTGGC CAGCAGCGAC TACGGGCAGA CGGTGGACGC GGTGGAGAAG
    TTGCTGAAAC GGCACGAAGC CTTTGAGAAG TCCACGGCCA CGTGGGAGGA GCGCATCGCT
    GCCCTGAGGA AGCTGACGAC GCTGGAGCTC ATGGGCGGGC GGACGCTGCG GGAGGAGGAC
    GTGGTGCACG ACGCTGCTCC CGAACGTCAG CTGGATCTGG ATGCAGAGCT GGAGGCAGGG
    TCTGAGGAGG AGGAGGAGAA GAGGAAGGGG GTGATCCCTC AGGAGGCCTC TCCGCCCGCT
    GTCGATGGAC CCCAGCCGCT GACAGTGCCC GAGGAGGAGC CCGTAGCACC GCGGGAGGAG
    GTGGCCACAT TGCCCGCCCA GCCTGCCCAC GTTCAGCTGG AGGGCTACCT GGGCCGCAAG
    CACGACCTGG AGGCGGCCAC CAAGCGTGCG TCCAACAGGT CGTGGAGCAC GCGGTACTGC
    GTGCTGCGGG GCGAGGAGCT TGCCTTCTTC AAGGACGCCA AGAGCCGGGC ACTGGGGGTG
    CCCTGCCAGG GTGAGGAGCC CCTGGGGCTG CGCAACGCAC GCTGCCAGGT GGCTGCTGGC
    TACAAGAAGA AGAAGCACGT CTTTGCGCTC AGGCTCAGCA ATGGCAGTGA GTGGCTCTTC
    CACGGCAAGG ATGAGGAGGA GATGCAGACG TGGCTGCAGG GGCTGCACGT GGCCATCACC
    TCCTGTCAGA ACCTGCCGGC CAAGGCGCGC AGCCTGCCCC CGCCCCACGC CCCCCCCGAG
    CCCCGGAGGG ACAAGGAGAA GCGCTTCAGC TTCTTCCCCA AGAAGAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa151189
    Clone ID Related Accession (Same CDS sequence) XM_015286287.2
    Accession Version XM_015286287.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6888bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product spectrin beta chain, erythrocytic isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015286287.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGACCTCCG AGAATGACTA CGACCATCTG GAGCTGCAGC AGTCCTACAG CCACTGGGAT 
    GCCTCCGACG ATGAGTTGGA CAACGACAAC AGCTCAGCGC GGCTCTTTGA GCGCTCCCGC
    ATCAAAGCTC TGGCAGATGA GCGGGAGGCG GTGCAGAAGA AGACCTTCAC CAAGTGGGTG
    AATCTGCACT TGGCGTGCGT CACCTGCCGC ATCTCCGACC TCTACCTGGA CCTCCGGGAC
    GGGCGGATGC TCATCAAGCT GCTGGAGGTG CTGTCCAAAG AGATGCTGCC CAAGCCCACC
    AAGGGCCGGA TGCGCATCCA CTGCCTGGAG AACGTGGACA AGGCACTGCA GTTCCTGAAG
    GAGAAGCAAG TGCACCTGGA GAACATGGGC TCCCACGACA TCGTGGATGG CAACCACCGC
    CTCATCCTCG GCCTCATCTG GACCATCATC CTCCGCTTCC AGGTGCAAGG CGTCATCGAG
    GATATGGAGG AGGATACAGA GACACGCTCG CCCAGGGAAG CGCTGCTGCT CTGGTGCCAA
    ATGAAGACGA AAGGCTACCC TCACGTGAAT GTCACCAACT TCACCTCCAG CTGGAAGGAT
    GGACTGGCCT TCAACGCCCT CATCCACAAG CACAGGCCTG AGCTGTTTGA CTTCAAAACT
    CTGACCAAGT CCAACGCCCG GCACAACCTG GAGCATGCCT TCAGCATGGC CGAGCGGCAC
    CTGGGCATCA CCCCGCTCCT CGACCCTGAA GATGTGTTCA CGGAGAACCC CGATGAGAAG
    TCCATCATCA CCTACGTGGT GGCCTTCTAC CACTACTTCT CCGAGATGAA GAAACTGGAG
    GTGAAGGGCA GGCGGCTGGG CAAGGTCATT GAGCACGCCA AAGAGACCAA GCGGATGATC
    GAGGGCTACG GGGGCCTGGC CTCCAACCTG CTCACCTGGA TCGAGCAGAC CATCATCTCC
    CTCAACAGCC GCAGCTTTGC CAACTCGCTG GCCGGTGTGC AGCACCAGCT GCAGACCTTC
    AGCACCTACC GCACGGTGGA GAAGCCCCCC AAGTTTCAGG AGAAGGGCAA CCTGGAGGTG
    CTGCTCTTCA CCATCCAGTC TCGGATGCGA GCCAACAACC AGCGTGTCTA CACACCACAA
    GAGGGGCGTC TGGTCTGTGA CATCAACCGG GCATGGGAGA AGCTGGAGAA AGCAGAGCAT
    GAGCGGGAGC TGGCACTGCG CAATGAGCTC ATCCGGCAGG AGAAGCTGGA GCAGCTGGCA
    CGGCGCTTTG ACCGCAAAGC AGCCATGCGG GAGGCCTGGC TGAGTGAGAA CCAACGCCTG
    GTGGCACAGG ATAACTTTGG CCACGACCTG ACGGCGGTGG AGGCGGCCAA GAAGAAACAC
    GAGGCCATTG AGACGGACAC AGCTGCCTAC ATGGAACGGG TGCAGGCCAT TGATGCAGTG
    GCGAAGGAGC TGGAGAGGGA GGGCTACCAC GACATCAAAC GCATCAGGGG GCGCAAGGAC
    AACATCCTGC AGCACTGGGA GTACCTCCAG GAGCTGCTGC GCAACCGGCG CCAGCGCCTT
    GAGATGAACC TCACCCTGCA GCACCTCTTC CAGGAGATGC TGCACAGCAT CAACTGGATG
    GATGAGGTCA AGGTGCAGCT GGCTTCATCC AAATCTGGGA AGCACCTCCT TGAGGTGGAG
    GAGCTTCTGG AGACCCACCG GCTGCTGGAA AGTGACATGG CCCTGCAGGC AGAGAAGGTG
    CGGGCCATCA GCACTGCTGC CCTCCGATTT GCCGACGCTG AGGGCTACCG CCCCTGCGAC
    CCCAGAGTGA TCCGAGACCG CGTGAACCAC CTGGAGATGT GCCGGCGGGA GCTGCAGGCA
    CTGGCGGCGA GGCGTAAAGC CCTGCTGGAG CAGTCCCGGG CTGTCTGGCA GTGTCTGCAG
    GAGCTGGACG AGGCAGAGAG CTGGATCAAG GAGCAGGAGC AGATCTACTC AGCGCTGGAC
    TACGGCAAAG ACCTGGCGGG CGTGCTGCTG CTGCGACGCC GTCACACCGC GCTGGAGGCT
    GAGCTGGAGG CACGGGGAGC CCGGCTGGAC AAGGCGCTGG TGATGGCTGA GCAGCTGGCA
    GCAGCAGGGC GTGAGGCCGG GCGGCTGCGG GACCGGGCGG CTGCCGTGCG GGTGCTGTGG
    GACCAGCTGC AGGAGCTGGT GGCCTTCCGC CGCCGCGGGC TGCGGGATGC CGAGGGCTTC
    TTCCAGTTCC AGGCAGAGGC TGAAGAGCTG TCAGAGGTTC TTACGGAGGC TCAGCAGCGG
    GCGGCCAGCG AGGAGCTGGG TCACGACGAG GTCCACACGC AGGCACTGCT GCGGGAGCAC
    CAGGAGCTGC TGGAGGAGCT GGCGGCTGCC CAGCAGCTCC TGGAGCGCTT GGGTCACCAA
    GCAGAGGGCT TCCCACCAGA GCTGCGGGCC GGACCCGAGG CGCACAACCG GCTGGCAGCG
    CTGCGGGAGC TGCACCACAA GGTGAGCACG CTGGCCGAGA CGCGTGGCCG AAGGCTGCGG
    GATGCCCTCA ACCTCTACAC TGTTTTTGGA GAGAGCGAAG CCTGCCAGCT CTGGATGGGC
    GCCAAGGAGC GGTGGCTGGA GAAGCTGGAG GTGCCCAACA AGCTGGAGGA CCTGGACGTG
    GTGAAGCACA GGCTGGATGG GCTGGAGCAG GAGATGGTTG GCGTGGCTTC TCAAATCGAC
    GCTGTCAACC GCTCAGCTGA CGGTCTGCTG GAGAGCGGCC ACCCACGCAG CCCACAGGTC
    CGGCAGTGCC AGCAGCAGCT CAATGAGCGG TGGGACCGTT TTCGGGAGCT GGTGTCCCAG
    TGGCGTTCAG CAGTGGGCTC GGCGCTCAAC CTGCTCAGCT TCCAACTGGA GTGCGAGGAG
    ACGCGCGCCT GGCTGCTGAG CAAGACACGG GTGGTGGAGT CCACAAAGGA GCTCGGCCGT
    GATCTGGCTG GAGTCCTGGC CACCCAGCGC AAGCTGTATG GCATTGAACG TGAGCTGGCA
    GCAACCGAGA GCCGCCTGGG TGCCCTGCGC CCGCAAGCCG CCCTCCTGGC CCAGGAGCGC
    CCTGAGTTGG CCGAGGACAC GGCACGGCGT CTGGCGGGGG CACAGGATGC CATGGACGGG
    CTGCAGGGCG CCCTGCGGGA CCGAGCGGCT GCGCTGGGAG AAGTGGGGCA GCTGCAGAGC
    TTCCTGCAGG ACCTGGATGA CTTCCAAGCG TGGCTCTTTG GGGCGCAGAA AGCCGTGGCA
    GGCACCGAAG AGGTGCCGAC TTCGGTGGGT GAGGCAGAAG AGCTGCTGCG GAAGCATGCG
    GCTGCCCTCG AGGATGCCGA GGGGCACACA GCTGCCTTCA CGGCGCTGCT GGAGGCAGGC
    GAGCGGGTGA CAAGCAACCA GGAGGACCCC GAGTATGAGC AGCTGCGGGA GAGGCTGCGG
    GGCGTGGAGG CGGGCTGGGG AGCCCTGCAC AAGATGTGGG ATGCCCGGCA GCGCTTCCTT
    GCCCAGTGCT TGGGATTCCA GGAGTTCCTG CGTGACGCCA AACAGGCTGA GATTCTCCTC
    GCCAACCAGG AGTACACGCT GGCCCACCTG GAGCTACCCC CTACACTGGA GGGTTCCACA
    GCTGCCCTGC GCCGCTTTGA GGATTTCCGT GCCTCCATGG AGAGCAGCGC TGAGAAGGTC
    CCTAGCGTGG TGGCCGCTGG CTCCAAGCTT GTGGCAGAGG GCAACATCTT CTCTGAGAAG
    ATCAGTGAGA AGAACCAGGC CCTGCAGGAG CGGCACCAGG TGAATGTGGC CAAGGCACAG
    GAGGCGGCGG GCTTGCTGCA GGACAACCAC AGGCTGCAGA GCTTCCTGCA GAGCTGCCGG
    GAGCTCGCTG CTTGGGTGGA TGAGAAGATG CTGACAGTGC AGGACGCGTC CTATGGAGGT
    GCACGTGGTT TGCATGGCAA GTGGCAGAAG CACCAAGCCT TTATGGCAGA GCTGGCAGCC
    AACGAGGCAT GGCTGGAGAA GATCAAAGCG GAGGGCATGG AGCTGGCGAG CTGCAAACCA
    CAGTACGGTG CAGTGGTGGG CCAGCGGCTG GACGAGCTGC AGGCGCTGTG GAATGGACTG
    CACGACGCTG CCAAGGAGAA GGGCCAGCAG CTCTTTGAGG CCAACCGCAC TGAGCTGTAC
    GCCCAGAGCT ACAGTGACCT GGAGCGCTGG CTGGGGCAGG TGGAGGGCGA GCTGCACACC
    ACTGAGCGTG CCAAGGACCT CACCAGCGCC AACCTACTGC TCAAGAAACT GACGCGGCTG
    GAGGAGCAGG TGGCGGCACG ACAGAAGGAA CTGATGGAGC TGAAAGCACC ACTTGCTGGG
    GTCGCACCAG AGCCCGATGC GCAGGAGGAG AAGCTCCAGC AGCGATTCCT TGACCTGCTG
    GAGCCACTGA GGAAGAAGAG GAAGGAGCTG GAAACCTCCA AGGCGATGTA CCAGCTGGGG
    AGGGACCTGG AGGACGAGAT GTTATGGGTG CAGGAGAGGA TGCCATGGGC CAGGTCAACT
    GAGAACGGCA CCAACCTCCA GACCGTGCAG CGTTTGGCCA AGAGGAATGA GACCCTACAG
    AAGGAGCTGC AGGGCCATGC CCCCCGCCTG GCCGAGGTGC TGGAACGTGG GGAGGCGGCT
    GCTGGGGGAC CCTGCCCGGA GCTGGCGGAG AGGGCACGGG AGCTGCAGGC GCAGTGGGAA
    GCGCTGAGGG AAGAAGTGGT CGCACGGCAG CAGCGGCTGC GGGAGGCCAG CGAGGCCCAG
    CAGTACTACC TGGATGCTGA TGAGGCCAAA GCGTGGGTCA GCGAGCAGGA GCTCTTCATG
    GGAGATGAGG AGAAGCCGAA GGACGAGGAA AGTGGTTTGG TGATGCTGAA GAGACACGTG
    AGGCAGCAGC GCTCCATCGA GGACTATGGG CAGACCATCA AGGAGCTGGC GGGGAGGGCT
    CAGCAGCTGC TCTCTGCTGG CCACCCTGAG GGGGAGCAGA TCATCCGGCT GCAGGGCCAG
    GTGGACAAGT ATTACGCGGG GCTGAAGGAG GCGGCCGAGG AGCGCCGGCG GCGCTTGGAG
    AACATGTGCC ACCTCTTCCA GCTGAAGCGT GAGGTGGAGG ACCTGGAGCA GTGGATTGCT
    GAGCGTGACG TGGCAGCCTC CTCCCAGGAG CTGGGGCAGG ACCTGGACCA TGTCACGATA
    CTGAGGGATA AGTTTCGGGA GTTTGCACGG GAAACAGGCA GCGTAGGGCA GGAACGTGTG
    GACCACGTCA ACCTGACCAT CGAGGACCTC ATCGACGCGG GGCACGCCGA GGCTGCCACC
    ATGGCTGAGT GGAAGGACGG ACTGAACGAG AGCTGGGCTG ACCTCCTGGA GCTGATTGAC
    ACACGCATGC AGTTGCTGGC CGCCTCCTAT GATCTGCGCA AGTACTTCTA TGACGGCTCT
    GAGCTGCTGG CCCTCATTGC TGCCCGCCAC CAGGAGCTGC CCCAGGACCT GGGCGACGAC
    TCGGGTTCGG TGGAGGCTTT CCACCGCATG CACAGCGCCT TCGAGCGTGA CCTGCAGCTG
    CTGGAGGGGC AGGTGCAGCG GTTTCGAGAG GTGGCAGCTC GCCTGCAAAC CGCCTATGCT
    GGGGAGAAGG CAGTCAGCAT CCAGCAGCAG GAGCAGGAGG TGGCACGGGC GCTGCAGGCA
    CTGCTGGAGG CATGCAGTGG GCGCCGGGCG CAGCTGGTGG ACACAGCCGA CAAGTTCCGC
    TTCTTCAGCA TGGCCCGTGA CCTGCTCTCC TGGATGGAGA GCACTGTGCG GCAGATCCAG
    ACACAGGAGA AGCCCAGGGA CGTTTCCTCG GTGGAGCTGC TGATGAAGTA CCACCAAGGC
    ATCCACGCCG AGATAGATGC CCGCGACAAG AGCTTTGATG CCTGCATTGA ACTGGGCAAG
    AAGCTGCTGC AGCGCAAACA CCGGGAGTCT CCTGAGATCA AGGCAAAGCT GATGGAGCTG
    CTGGACAAGA GGAAGGCCAT GATGGAGATG TGGCAGAAGC GCTGGGACCG GCTGCGGCTG
    CTGTTGGAGG TGTGCCAGTT CTCCCGGGAC GCCTCAGTGG CCGAGTCGTG GCTGATGGCA
    CAGGAGCCCT ACCTGGCCAG CAGCGACTAC GGGCAGACGG TGGACGCGGT GGAGAAGTTG
    CTGAAACGGC ACGAAGCCTT TGAGAAGTCC ACGGCCACGT GGGAGGAGCG CATCGCTGCC
    CTGAGGAAGC TGACGACGCT GGAGCTCATG GGCGGGCGGA CGCTGCGGGA GGAGGACGTG
    GTGCACGACG CTGCTCCCGA ACGTCAGCTG GATCTGGATG CAGAGCTGGA GGCAGGGTCT
    GAGGAGGAGG AGGAGAAGAG GAAGGGGGTG ATCCCTCAGG AGGCCTCTCC GCCCGCTGTC
    GATGGACCCC AGCCGCTGAC AGTGCCCGAG GAGGAGCCCG TAGCACCGCG GGAGGAGGTG
    GCCACATTGC CCGCCCAGCC TGCCCACGTT CAGCTGGAGG GCTACCTGGG CCGCAAGCAC
    GACCTGGAGG CGGCCACCAA GCGTGCGTCC AACAGGTCGT GGAGCACGCG GTACTGCGTG
    CTGCGGGGCG AGGAGCTTGC CTTCTTCAAG GACGCCAAGA GCCGGGCACT GGGGGTGCCC
    TGCCAGGGTG AGGAGCCCCT GGGGCTGCGC AACGCACGCT GCCAGGTGGC TGCTGGCTAC
    AAGAAGAAGA AGCACGTCTT TGCGCTCAGG CTCAGCAATG GCAGTGAGTG GCTCTTCCAC
    GGCAAGGATG AGGAGGAGAT GCAGACGTGG CTGCAGGGGC TGCACGTGGC CATCACCTCC
    TGTCAGAACC TGCCGGCCAA GGCGCGCAGC CTGCCCCCGC CCCACGCCCC CCCCGAGCCC
    CGGAGGGACA AGGAGAAGCG CTTCAGCTTC TTCCCCAAGA AGAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015141773.2
    CDS191..7078
    Translation

    Target ORF information:

    RefSeq Version XM_015286287.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spectrin beta, erythrocytic (SPTB), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015286287.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGACCTCCG AGAATGACTA CGACCATCTG GAGCTGCAGC AGTCCTACAG CCACTGGGAT 
    GCCTCCGACG ATGAGTTGGA CAACGACAAC AGCTCAGCGC GGCTCTTTGA GCGCTCCCGC
    ATCAAAGCTC TGGCAGATGA GCGGGAGGCG GTGCAGAAGA AGACCTTCAC CAAGTGGGTG
    AATCTGCACT TGGCGTGCGT CACCTGCCGC ATCTCCGACC TCTACCTGGA CCTCCGGGAC
    GGGCGGATGC TCATCAAGCT GCTGGAGGTG CTGTCCAAAG AGATGCTGCC CAAGCCCACC
    AAGGGCCGGA TGCGCATCCA CTGCCTGGAG AACGTGGACA AGGCACTGCA GTTCCTGAAG
    GAGAAGCAAG TGCACCTGGA GAACATGGGC TCCCACGACA TCGTGGATGG CAACCACCGC
    CTCATCCTCG GCCTCATCTG GACCATCATC CTCCGCTTCC AGGTGCAAGG CGTCATCGAG
    GATATGGAGG AGGATACAGA GACACGCTCG CCCAGGGAAG CGCTGCTGCT CTGGTGCCAA
    ATGAAGACGA AAGGCTACCC TCACGTGAAT GTCACCAACT TCACCTCCAG CTGGAAGGAT
    GGACTGGCCT TCAACGCCCT CATCCACAAG CACAGGCCTG AGCTGTTTGA CTTCAAAACT
    CTGACCAAGT CCAACGCCCG GCACAACCTG GAGCATGCCT TCAGCATGGC CGAGCGGCAC
    CTGGGCATCA CCCCGCTCCT CGACCCTGAA GATGTGTTCA CGGAGAACCC CGATGAGAAG
    TCCATCATCA CCTACGTGGT GGCCTTCTAC CACTACTTCT CCGAGATGAA GAAACTGGAG
    GTGAAGGGCA GGCGGCTGGG CAAGGTCATT GAGCACGCCA AAGAGACCAA GCGGATGATC
    GAGGGCTACG GGGGCCTGGC CTCCAACCTG CTCACCTGGA TCGAGCAGAC CATCATCTCC
    CTCAACAGCC GCAGCTTTGC CAACTCGCTG GCCGGTGTGC AGCACCAGCT GCAGACCTTC
    AGCACCTACC GCACGGTGGA GAAGCCCCCC AAGTTTCAGG AGAAGGGCAA CCTGGAGGTG
    CTGCTCTTCA CCATCCAGTC TCGGATGCGA GCCAACAACC AGCGTGTCTA CACACCACAA
    GAGGGGCGTC TGGTCTGTGA CATCAACCGG GCATGGGAGA AGCTGGAGAA AGCAGAGCAT
    GAGCGGGAGC TGGCACTGCG CAATGAGCTC ATCCGGCAGG AGAAGCTGGA GCAGCTGGCA
    CGGCGCTTTG ACCGCAAAGC AGCCATGCGG GAGGCCTGGC TGAGTGAGAA CCAACGCCTG
    GTGGCACAGG ATAACTTTGG CCACGACCTG ACGGCGGTGG AGGCGGCCAA GAAGAAACAC
    GAGGCCATTG AGACGGACAC AGCTGCCTAC ATGGAACGGG TGCAGGCCAT TGATGCAGTG
    GCGAAGGAGC TGGAGAGGGA GGGCTACCAC GACATCAAAC GCATCAGGGG GCGCAAGGAC
    AACATCCTGC AGCACTGGGA GTACCTCCAG GAGCTGCTGC GCAACCGGCG CCAGCGCCTT
    GAGATGAACC TCACCCTGCA GCACCTCTTC CAGGAGATGC TGCACAGCAT CAACTGGATG
    GATGAGGTCA AGGTGCAGCT GGCTTCATCC AAATCTGGGA AGCACCTCCT TGAGGTGGAG
    GAGCTTCTGG AGACCCACCG GCTGCTGGAA AGTGACATGG CCCTGCAGGC AGAGAAGGTG
    CGGGCCATCA GCACTGCTGC CCTCCGATTT GCCGACGCTG AGGGCTACCG CCCCTGCGAC
    CCCAGAGTGA TCCGAGACCG CGTGAACCAC CTGGAGATGT GCCGGCGGGA GCTGCAGGCA
    CTGGCGGCGA GGCGTAAAGC CCTGCTGGAG CAGTCCCGGG CTGTCTGGCA GTGTCTGCAG
    GAGCTGGACG AGGCAGAGAG CTGGATCAAG GAGCAGGAGC AGATCTACTC AGCGCTGGAC
    TACGGCAAAG ACCTGGCGGG CGTGCTGCTG CTGCGACGCC GTCACACCGC GCTGGAGGCT
    GAGCTGGAGG CACGGGGAGC CCGGCTGGAC AAGGCGCTGG TGATGGCTGA GCAGCTGGCA
    GCAGCAGGGC GTGAGGCCGG GCGGCTGCGG GACCGGGCGG CTGCCGTGCG GGTGCTGTGG
    GACCAGCTGC AGGAGCTGGT GGCCTTCCGC CGCCGCGGGC TGCGGGATGC CGAGGGCTTC
    TTCCAGTTCC AGGCAGAGGC TGAAGAGCTG TCAGAGGTTC TTACGGAGGC TCAGCAGCGG
    GCGGCCAGCG AGGAGCTGGG TCACGACGAG GTCCACACGC AGGCACTGCT GCGGGAGCAC
    CAGGAGCTGC TGGAGGAGCT GGCGGCTGCC CAGCAGCTCC TGGAGCGCTT GGGTCACCAA
    GCAGAGGGCT TCCCACCAGA GCTGCGGGCC GGACCCGAGG CGCACAACCG GCTGGCAGCG
    CTGCGGGAGC TGCACCACAA GGTGAGCACG CTGGCCGAGA CGCGTGGCCG AAGGCTGCGG
    GATGCCCTCA ACCTCTACAC TGTTTTTGGA GAGAGCGAAG CCTGCCAGCT CTGGATGGGC
    GCCAAGGAGC GGTGGCTGGA GAAGCTGGAG GTGCCCAACA AGCTGGAGGA CCTGGACGTG
    GTGAAGCACA GGCTGGATGG GCTGGAGCAG GAGATGGTTG GCGTGGCTTC TCAAATCGAC
    GCTGTCAACC GCTCAGCTGA CGGTCTGCTG GAGAGCGGCC ACCCACGCAG CCCACAGGTC
    CGGCAGTGCC AGCAGCAGCT CAATGAGCGG TGGGACCGTT TTCGGGAGCT GGTGTCCCAG
    TGGCGTTCAG CAGTGGGCTC GGCGCTCAAC CTGCTCAGCT TCCAACTGGA GTGCGAGGAG
    ACGCGCGCCT GGCTGCTGAG CAAGACACGG GTGGTGGAGT CCACAAAGGA GCTCGGCCGT
    GATCTGGCTG GAGTCCTGGC CACCCAGCGC AAGCTGTATG GCATTGAACG TGAGCTGGCA
    GCAACCGAGA GCCGCCTGGG TGCCCTGCGC CCGCAAGCCG CCCTCCTGGC CCAGGAGCGC
    CCTGAGTTGG CCGAGGACAC GGCACGGCGT CTGGCGGGGG CACAGGATGC CATGGACGGG
    CTGCAGGGCG CCCTGCGGGA CCGAGCGGCT GCGCTGGGAG AAGTGGGGCA GCTGCAGAGC
    TTCCTGCAGG ACCTGGATGA CTTCCAAGCG TGGCTCTTTG GGGCGCAGAA AGCCGTGGCA
    GGCACCGAAG AGGTGCCGAC TTCGGTGGGT GAGGCAGAAG AGCTGCTGCG GAAGCATGCG
    GCTGCCCTCG AGGATGCCGA GGGGCACACA GCTGCCTTCA CGGCGCTGCT GGAGGCAGGC
    GAGCGGGTGA CAAGCAACCA GGAGGACCCC GAGTATGAGC AGCTGCGGGA GAGGCTGCGG
    GGCGTGGAGG CGGGCTGGGG AGCCCTGCAC AAGATGTGGG ATGCCCGGCA GCGCTTCCTT
    GCCCAGTGCT TGGGATTCCA GGAGTTCCTG CGTGACGCCA AACAGGCTGA GATTCTCCTC
    GCCAACCAGG AGTACACGCT GGCCCACCTG GAGCTACCCC CTACACTGGA GGGTTCCACA
    GCTGCCCTGC GCCGCTTTGA GGATTTCCGT GCCTCCATGG AGAGCAGCGC TGAGAAGGTC
    CCTAGCGTGG TGGCCGCTGG CTCCAAGCTT GTGGCAGAGG GCAACATCTT CTCTGAGAAG
    ATCAGTGAGA AGAACCAGGC CCTGCAGGAG CGGCACCAGG TGAATGTGGC CAAGGCACAG
    GAGGCGGCGG GCTTGCTGCA GGACAACCAC AGGCTGCAGA GCTTCCTGCA GAGCTGCCGG
    GAGCTCGCTG CTTGGGTGGA TGAGAAGATG CTGACAGTGC AGGACGCGTC CTATGGAGGT
    GCACGTGGTT TGCATGGCAA GTGGCAGAAG CACCAAGCCT TTATGGCAGA GCTGGCAGCC
    AACGAGGCAT GGCTGGAGAA GATCAAAGCG GAGGGCATGG AGCTGGCGAG CTGCAAACCA
    CAGTACGGTG CAGTGGTGGG CCAGCGGCTG GACGAGCTGC AGGCGCTGTG GAATGGACTG
    CACGACGCTG CCAAGGAGAA GGGCCAGCAG CTCTTTGAGG CCAACCGCAC TGAGCTGTAC
    GCCCAGAGCT ACAGTGACCT GGAGCGCTGG CTGGGGCAGG TGGAGGGCGA GCTGCACACC
    ACTGAGCGTG CCAAGGACCT CACCAGCGCC AACCTACTGC TCAAGAAACT GACGCGGCTG
    GAGGAGCAGG TGGCGGCACG ACAGAAGGAA CTGATGGAGC TGAAAGCACC ACTTGCTGGG
    GTCGCACCAG AGCCCGATGC GCAGGAGGAG AAGCTCCAGC AGCGATTCCT TGACCTGCTG
    GAGCCACTGA GGAAGAAGAG GAAGGAGCTG GAAACCTCCA AGGCGATGTA CCAGCTGGGG
    AGGGACCTGG AGGACGAGAT GTTATGGGTG CAGGAGAGGA TGCCATGGGC CAGGTCAACT
    GAGAACGGCA CCAACCTCCA GACCGTGCAG CGTTTGGCCA AGAGGAATGA GACCCTACAG
    AAGGAGCTGC AGGGCCATGC CCCCCGCCTG GCCGAGGTGC TGGAACGTGG GGAGGCGGCT
    GCTGGGGGAC CCTGCCCGGA GCTGGCGGAG AGGGCACGGG AGCTGCAGGC GCAGTGGGAA
    GCGCTGAGGG AAGAAGTGGT CGCACGGCAG CAGCGGCTGC GGGAGGCCAG CGAGGCCCAG
    CAGTACTACC TGGATGCTGA TGAGGCCAAA GCGTGGGTCA GCGAGCAGGA GCTCTTCATG
    GGAGATGAGG AGAAGCCGAA GGACGAGGAA AGTGGTTTGG TGATGCTGAA GAGACACGTG
    AGGCAGCAGC GCTCCATCGA GGACTATGGG CAGACCATCA AGGAGCTGGC GGGGAGGGCT
    CAGCAGCTGC TCTCTGCTGG CCACCCTGAG GGGGAGCAGA TCATCCGGCT GCAGGGCCAG
    GTGGACAAGT ATTACGCGGG GCTGAAGGAG GCGGCCGAGG AGCGCCGGCG GCGCTTGGAG
    AACATGTGCC ACCTCTTCCA GCTGAAGCGT GAGGTGGAGG ACCTGGAGCA GTGGATTGCT
    GAGCGTGACG TGGCAGCCTC CTCCCAGGAG CTGGGGCAGG ACCTGGACCA TGTCACGATA
    CTGAGGGATA AGTTTCGGGA GTTTGCACGG GAAACAGGCA GCGTAGGGCA GGAACGTGTG
    GACCACGTCA ACCTGACCAT CGAGGACCTC ATCGACGCGG GGCACGCCGA GGCTGCCACC
    ATGGCTGAGT GGAAGGACGG ACTGAACGAG AGCTGGGCTG ACCTCCTGGA GCTGATTGAC
    ACACGCATGC AGTTGCTGGC CGCCTCCTAT GATCTGCGCA AGTACTTCTA TGACGGCTCT
    GAGCTGCTGG CCCTCATTGC TGCCCGCCAC CAGGAGCTGC CCCAGGACCT GGGCGACGAC
    TCGGGTTCGG TGGAGGCTTT CCACCGCATG CACAGCGCCT TCGAGCGTGA CCTGCAGCTG
    CTGGAGGGGC AGGTGCAGCG GTTTCGAGAG GTGGCAGCTC GCCTGCAAAC CGCCTATGCT
    GGGGAGAAGG CAGTCAGCAT CCAGCAGCAG GAGCAGGAGG TGGCACGGGC GCTGCAGGCA
    CTGCTGGAGG CATGCAGTGG GCGCCGGGCG CAGCTGGTGG ACACAGCCGA CAAGTTCCGC
    TTCTTCAGCA TGGCCCGTGA CCTGCTCTCC TGGATGGAGA GCACTGTGCG GCAGATCCAG
    ACACAGGAGA AGCCCAGGGA CGTTTCCTCG GTGGAGCTGC TGATGAAGTA CCACCAAGGC
    ATCCACGCCG AGATAGATGC CCGCGACAAG AGCTTTGATG CCTGCATTGA ACTGGGCAAG
    AAGCTGCTGC AGCGCAAACA CCGGGAGTCT CCTGAGATCA AGGCAAAGCT GATGGAGCTG
    CTGGACAAGA GGAAGGCCAT GATGGAGATG TGGCAGAAGC GCTGGGACCG GCTGCGGCTG
    CTGTTGGAGG TGTGCCAGTT CTCCCGGGAC GCCTCAGTGG CCGAGTCGTG GCTGATGGCA
    CAGGAGCCCT ACCTGGCCAG CAGCGACTAC GGGCAGACGG TGGACGCGGT GGAGAAGTTG
    CTGAAACGGC ACGAAGCCTT TGAGAAGTCC ACGGCCACGT GGGAGGAGCG CATCGCTGCC
    CTGAGGAAGC TGACGACGCT GGAGCTCATG GGCGGGCGGA CGCTGCGGGA GGAGGACGTG
    GTGCACGACG CTGCTCCCGA ACGTCAGCTG GATCTGGATG CAGAGCTGGA GGCAGGGTCT
    GAGGAGGAGG AGGAGAAGAG GAAGGGGGTG ATCCCTCAGG AGGCCTCTCC GCCCGCTGTC
    GATGGACCCC AGCCGCTGAC AGTGCCCGAG GAGGAGCCCG TAGCACCGCG GGAGGAGGTG
    GCCACATTGC CCGCCCAGCC TGCCCACGTT CAGCTGGAGG GCTACCTGGG CCGCAAGCAC
    GACCTGGAGG CGGCCACCAA GCGTGCGTCC AACAGGTCGT GGAGCACGCG GTACTGCGTG
    CTGCGGGGCG AGGAGCTTGC CTTCTTCAAG GACGCCAAGA GCCGGGCACT GGGGGTGCCC
    TGCCAGGGTG AGGAGCCCCT GGGGCTGCGC AACGCACGCT GCCAGGTGGC TGCTGGCTAC
    AAGAAGAAGA AGCACGTCTT TGCGCTCAGG CTCAGCAATG GCAGTGAGTG GCTCTTCCAC
    GGCAAGGATG AGGAGGAGAT GCAGACGTGG CTGCAGGGGC TGCACGTGGC CATCACCTCC
    TGTCAGAACC TGCCGGCCAA GGCGCGCAGC CTGCCCCCGC CCCACGCCCC CCCCGAGCCC
    CGGAGGGACA AGGAGAAGCG CTTCAGCTTC TTCCCCAAGA AGAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa36099
    Clone ID Related Accession (Same CDS sequence) XM_015286286.1
    Accession Version XM_015286286.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6891bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product spectrin beta chain, erythrocytic isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455869.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGACCTCCG AGAATGACTA CGACCATCTG GAGCTGCAGC AGTCCTACAG CCACTGGGAT 
    GCCTCCGACG ATGAGTTGGA CAACGACAAC AGCTCAGCGC GGCTCTTTGA GCGCTCCCGC
    ATCAAAGCTC TGGCAGATGA GCGGGAGGCG GTGCAGAAGA AGACCTTCAC CAAGTGGGTG
    AACCTGCACT TGGCGTGCGT CACCTGCCGC ATCTCCGACC TCTACCTGGA CCTCCGGGAC
    GGGCGGATGC TCATCAAGCT GCTGGAGGTG CTGTCCAAAG AGATGCTGCC CAAGCCCACC
    AAGGGCCGGA TGCGCATCCA CTGCCTGGAG AACGTGGACA AAGCACTGCA GTTCCTGAAG
    GAGCAGCGAG TGCACCTGGA GAACATGGGC TCCCACGACA TCGTGGATGG CAACCACCGC
    CTCATCCTCG GCCTCATCTG GACCATCATC CTCCGCTTCC AGGTGCAAGG CGTCATCGAG
    GATATGGAGG AGGATACAGA GACACGCTCG CCCAGGGAAG CGCTGCTGCT CTGGTGCCAA
    ATGAAGACGA AAGGCTACCC TCACGTGAAT GTCACCAACT TCACCTCCAG CTGGAAGGAT
    GGACTGGCCT TCAACGCCCT CATCCACAAG CACAGGCCTG AGCTGTTTGA CTTCAAAACT
    CTGACCAAGT CCAACGCCCG GCACAACCTG GAGCATGCCT TCAGCATGGC CGAGAGGCAC
    CTGGGCATCA CCCCGCTCCT CGACCCTGAA GATGTGTTCA CGGAGAACCC CGATGAGAAG
    TCCATCATCA CCTACGTGGT GGCCTTCTAC CACTACTTCT CCGAGATGAA GAAACTGGAG
    GTGAAGGGCA GGCGGCTGGG CAAGGTCATT GAGCACGCCA AAGAGACCAA GCGGATGATC
    GAGGGCTACG GGGGCCTGGC CTCCAACCTG CTCACCTGGA TCGAGCAGAC CATCATCTCC
    CTCAACAGCC GCAGCTTTGC CAACTCGCTG GCCGGTGTGC AGCACCAGCT GCAGACCTTC
    AGCACCTACC GCACGGTGGA GAAGCCCCCC AAGTTTCAGG AGAAGGGCAA CCTGGAGGTG
    CTGCTCTTCA CCATCCAGTC TCGGATGCGA GCCAACAACC AGCGTGTCTA CACACCACAA
    GAGGGGCGTC TGGTCTGTGA CATCAACCGG GCATGGGAGA AGCTGGAGAA AGCAGAGCAT
    GAGCGGGAGC TGGCACTGCG CAATGAGCTC ATCCGGCAGG AGAAGCTGGA GCAGCTGGCA
    CGGCGCTTTG ACCGCAAAGC AGCCATGCGG GAGGCCTGGC TGAGTGAGAA CCAACGCCTG
    GTGGCACAGC AGGATAACTT TGGCCACGAC CTGACGGCGG TGGAGGCGGC CAAGAAGAAA
    CACGAGGCCA TTGAGACGGA CACAGCTGCC TACATGGAAC GGGTGCAGGC CATTGATGCA
    GTGGCGAAGG AGCTGGAGAG GGAGGGCTAC CACGACATCA AACGCATCAG GGGGCGCAAG
    GACAACATCC TGCAGCACTG GGAGTACCTC CAGGAGCTGC TGCGCAACCG GCGCCAGCGC
    CTTGAGATGA ACCTCACCCT GCAGCACCTC TTCCAGGAGA TGCTGCACAG CATCAACTGG
    ATGGATGAGG TCAAGGTGCA GCTGGCCTCA TCCAAATCTG GGAAGCACCT CCTTGAGGTG
    GAGGAGCTTC TGGAGACCCA CCGGCTACTG GAAAGTGACA TGGCCCTGCA GGCAGAGAAG
    GTGCGGGCCG TCAGCACTGC TGCCCTCCGA TTTGCCGACG CTGAGGGCTA CCGCCCCTGC
    GACCCCAGAG TGATCCGAGA CCGCGTGAAC CACCTGGAGA TGTGCCGGCG GGAGCTGCAG
    GCACTGGCGG CGAGGCGTAA AGCCCTGCTG GAGCAGTCCC GGGCTGTCTG GCAGTGTCTG
    CAGGAGCTGG ACGAGGCAGA GAGCTGGATC AAGGAGCAGG AGCAGATCTA CTCAGCGCTG
    GACTACGGCA AAGACCTGGC GGGCGTGCTG CTGCTGCGAC GCCGTCACAC CGCGCTGGAG
    GCTGAGCTGG AGGCACGGGG AGCCCGGCTG GACAAGGCGC TGGTGATGGC TGAGCAGCTG
    GCAGCAGCAG GGCGTGAGGC CGGGCGGCTG CGGGACCGGG CGGCTGCCGT GCGGGTGCTG
    TGGGACCAGC TGCAGGAGCT GGTGGCCTTC CGCCGCCGCG GGCTGCGGGA TGCCGAGGGC
    TTCTTCCAGT TCCAGGCAGA GGCTGAAGAG CTGGCAGAGG TTCTTACGGA GGCTCAGCAG
    CGGGCGGCCA GCGAGGAGCT GGGTCACGAC GAGGTCCACA CGCAGGCACT GCTGCGGGAG
    CACCAGGAGC TGCTGGAGGA GCTGGCGGCT GCCCAGCAGC TCCTGGAGCG CTTGGGTCAC
    CAAGCAGAGG GCTTCCCACC AGAGCTGCGG GCCGGACCCG AGGCGCACAA CCGGCTGGCA
    GCGCTGCGGG AGCTGCACCA CAAGGTGAGC ACGCTGGCCG AGACGCGTGG CCGAAGGCTG
    CGGGATGCCC TCAACCTCTA CACTGTTTTT GGAGAGAGCG AAGCCTGCCA GCTCTGGATG
    GGCGCCAAGG AGCGGTGGCT GGAGAAGCTG GAGGTGCCCA ACAAGCTGGA GGACCTGGAC
    GTGGTGAAGC ACAGGCTGGA TGGGCTGGAG CAGGAGATGG TTGGCGTGGC TTCTCAAATC
    GACGCTGTCA ACCGCTCAGC TGACGGTCTG CTGGAGAGCG GCCACCCACG CAGCCCACAG
    GTCCGGCAGT GCCAGCAGCA GCTCAATGAG CGGTGGGACC GTTTTCGGGA GCTGGTGTCC
    CAGTGGCGTG CAGCAGTGGG CTCGGCGCTC AACCTGCTCA GCTTCCAACT GGAGTGCGAG
    GAGACGCGCG CCTGGCTGCT GAGCAAGACA CGGGTGGTGG AGTCCACAAA GGAGCTCGGC
    CGTGATCTGG CTGGAGTCCT GGCCACCCAG CGCAAGCTGT ATGGCATTGA ACGTGAGCTG
    GCAGCAACCG AGAGCCGCCT GGGTGCCCTG CGCCCGCAAG CCGCCCTCCT GGCCCAGGAG
    CGCCCTGAGT TGGCCGAGGA CACGGCACGG CGTCTGGCGG GGGCACAGGA TGCCATGGAC
    GGGCTGCAGG GCGCCCTGCG GGACCGAGCG GCTGCGCTGG GAGAAGTGGG GCAGCTGCAG
    AGCTTCCTGC AGGACCTGGA TGACTTCCAA GCGTGGCTCT TTGGGGCGCA GAAAGCCGTG
    GCAGGCACCG AAGAGGTGCC GACTTCGGTG GGTGAGGCAG AAGAGCTGCT GCGGAAGCAT
    GCGGCTGCCC TCGAGGATGC CGAGGGGCAC ACAGCTGCCT TCACGGCGCT GCTGGAGGCA
    GGCGAGCGGG TGACAAGCAA CCAGGAGGAC CCCGAGTATG AGCAGCTGCG GGAGAGGCTG
    CGGGGCGTGG AGGCGGGCTG GGGAGCCCTG CACAAGATGT GGGATGCCCG GCAGCGCTTC
    CTTGCCCAGT GCTTGGGATT CCAGGAGTTC CTGCGTGACG CCAAACAGGC TGAGATTCTC
    CTCGCCAACC AGGAGTACAC GCTGGCCCAC CTGGAGCTAC CCCCTACACT GGAGGGTTCT
    ACAGCTGCCC TGCGCCGCTT TGAGGATTTC CGTGCCTCCA TGGAGAGCAG CGCTGAGAAG
    GTCCCTAGCG TGGTGGCCGC TGGCTCCAAG CTTGTGGCAG AGGGCAACAT CTTCTCTGAG
    AAGATCAGTG AGAAGAACCA GGCCCTGCAG GAGCGGCACC AGGTGAATGT GGCCAAGGCA
    CAGGAGGCGG CGGGCTTGCT GCAGGACAAC CACAGGCTGC AGAGCTTCCT GCAGAGCTGC
    CGGGAGCTCG CTGCTTGGGT GGATGAGAAG ATGCTGACAG TGCAGGACGC GTCCTATGGA
    GGTGCACGTG GTTTGCATGG CAAGTGGCAG AAGCACCAAG CCTTTATGGC AGAGCTGGCA
    GCCAACGAGG CATGGCTGGA GAAGATCAAA GCGGAGGGCA TGGAGCTGGC GAGCTGCAAA
    CCACAGTACG GTGCAGTGGT GGGCCAGCGG CTGGACGAGC TGCAGGCGCT GTGGAATGGA
    CTGCACGACG CTGCCAAGGA GAAGGGCCAG CAGCTCTTTG AGGCCAACCG CACTGAGCTG
    TACGCCCAGA GCTACAGTGA CCTGGAGCGC TGGCTGGGGC AGGTGGAGGG CGAGCTGCAC
    ACCACTGAGC GTGCCAAGGA CCTCACCAGC GCCAACCTAC TGCTCAAGAA ACTGACGCGG
    CTGGAGGAGC AGGTGGCGGC ACGACAGAAG GAACTGATGG AGCTGAAAGC ACCACTTGCT
    GGGGTCGCAC CAGAGCCCGA TGCGCAGGAG GAGAAGCTCC AGCAGCGATT CCTTGACCTG
    CTGGAGCCAC TGAGGAAGAA GAGGAAGGAG CTGGAAACCT CCAAGGCGAT GTACCAGCTG
    GGGAGGGACC TGGAGGACGA GATGTTATGG GTGCAGGAGA GGATGCCATG GGCCAGGTCA
    ACTGAGAACG GCACCAACCT CCAGACCGTG CAGCGTTTGG CCAAGAGGAA TGAGACCCTA
    CAGAAGGAGC TGCAGGGCCA TGCCCCCCGC CTGGCCGAGG TGCTGGAACG TGGGGAGGCG
    GCTGCTGGGG GACCCTGCCC GGAGCTGGCG GAGAGGGCAC GGGAGCTGCA GGCGCAGTGG
    GAAGCGCTGA GGGAAGAAGT GGTCGCACGG CAGCAGCGGC TGCGGGAGGC CAGCGAGGCC
    CAGCAGTACT ACCTGGATGC TGATGAGGCC AAAGCGTGGG TCAGCGAGCA GGAGCTCTTC
    ATGGGAGATG AGGAGAAGCC GAAGGACGAG GAAAGTGGTT TGGTGATGCT GAAGAGACAC
    GTGAGGCAGC AGCGCTCCAT CGAGGACTAT GGGCAGACCA TCAAGGAGCT GGCGGGGAGG
    GCTCAGCAGC TGCTCTCTGC TGGCCACCCT GAGGGGGAGC AGATCATCCG GCTGCAGGGC
    CAGGTGGACA AGTATTACGC GGGGCTGAAG GAGGCGGCCG AGGAGCGCCG GCGGCGCTTG
    GAGAACATGT GCCACCTCTT CCAGCTGAAG CGTGAGGTGG AGGACCTGGA GCAGTGGATT
    GCTGAGCGTG ACGTGGCAGC CTCCTCCCAG GAGCTGGGGC AGGACCTGGA CCATGTCACG
    ATACTGAGGG ATAAGTTTCG GGAGTTTGCA CGGGAAACAG GCAGCGTAGG GCAGGAACGT
    GTGGACCACG TCAACCTGAC CATCGAGGAC CTCATCGACG CGGGGCACGC CGAGGCTGCC
    ACCATGGCTG AGTGGAAGGA CGGACTGAAC GAGAGCTGGG CTGACCTCCT GGAGCTGATT
    GACACACGCA TGCAGTTGCT GGCCGCCTCC TATGATCTGC GCAAGTACTT CTATGACGGC
    TCTGAGCTGC TGGCCCTCAT TGCTGCCCGC CACCAGGAGC TGCCCCAGGA CCTGGGCGAC
    GACTCGGGTT CGGTGGAGGC TTTCCACCGC ATGCACAGCG CCTTCGAGCG TGACCTGCAG
    CTGCTGGAGG GGCAGGTGCA GCGGTTTCGA GAGGTGGCAG CTCGCCTGCA AACCGCCTAT
    GCTGGGGAGA AGGCAGTCAG CATCCAGCAG CAGGAGCAGG AGGTGGCACG GGCGCTGCAG
    GCACTGCTGG AGGCATGCAG TGGGCGCCGG GCGCAGCTGG TGGACACAGC CGACAAGTTC
    CGCTTCTTCA GCATGGCCCG TGACCTGCTC TCCTGGATGG AGAGCACTGT GCGGCAGATC
    CAGACACAGG AGAAGCCCAG GGACGTTTCC TCGGTGGAGC TGCTGATGAA GTACCACCAA
    GGCATCCACG CCGAGATAGA TGCCCGCGAC AAGAGCTTTG ATGCCTGCAT TGAACTGGGC
    AAGAAGCTGC TGCAGCGCAA ACACCGGGAG TCTCCTGAGA TCAAGGCAAA GCTGATGGAG
    CTGCTGGACA AGAGGAAGGC CATGATGGAG ATGTGGCAGA AGCGCTGGGA CCGGCTGCGG
    CTGCTGTTGG AGGTGTGCCA GTTCTCCCGG GACGCCTCAG TGGCCGAGTC GTGGCTGATG
    GCACAGGAGC CCTACCTGGC CAGCAGCGAC TACGGGCAGA CGGTGGACGC GGTGGAGAAG
    TTGCTGAAAC GGCACGAAGC CTTTGAGAAG TCCACGGCCA CGTGGGAGGA GCGCATCGCT
    GCCCTGAGGA AGCTGACGAC GCTGGAGCTC ATGGGCGGGC GGACGCTGCG GGAGGAGGAC
    GTGGTGCACG ACGCTGCTCC CGAACGTCAG CTGGATCTGG ATGCAGAGCT GGAGGCAGGG
    TCTGAGGAGG AGGAGGAGAA GAGGAAGGGG GTGATCCCTC AGGAGGCCTC TCCGCCCGCT
    GTCGATGGAC CCCAGCCGCT GACAGTGCCC GAGGAGGAGC CCGTAGCACC GCGGGAGGAG
    GTGGCCACAT TGCCCGCCCA GCCTGCCCAC GTTCAGCTGG AGGGCTACCT GGGCCGCAAG
    CACGACCTGG AGGCGGCCAC CAAGCGTGCG TCCAACAGGT CGTGGAGCAC GCGGTACTGC
    GTGCTGCGGG GCGAGGAGCT TGCCTTCTTC AAGGACGCCA AGAGCCGGGC ACTGGGGGTG
    CCCTGCCAGG GTGAGGAGCC CCTGGGGCTG CGCAACGCAC GCTGCCAGGT GGCTGCTGGC
    TACAAGAAGA AGAAGCACGT CTTTGCGCTC AGGCTCAGCA ATGGCAGTGA GTGGCTCTTC
    CACGGCAAGG ATGAGGAGGA GATGCAGACG TGGCTGCAGG GGCTGCACGT GGCCATCACC
    TCCTGTCAGA ACCTGCCGGC CAAGGCGCGC AGCCTGCCCC CGCCCCACGC CCCCCCCGAG
    CCCCGGAGGG ACAAGGAGAA GCGCTTCAGC TTCTTCCCCA AGAAGAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015141772.1
    CDS196..7086
    Translation

    Target ORF information:

    RefSeq Version XM_015286286.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spectrin, beta, erythrocytic (SPTB), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015286286.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGACCTCCG AGAATGACTA CGACCATCTG GAGCTGCAGC AGTCCTACAG CCACTGGGAT 
    GCCTCCGACG ATGAGTTGGA CAACGACAAC AGCTCAGCGC GGCTCTTTGA GCGCTCCCGC
    ATCAAAGCTC TGGCAGATGA GCGGGAGGCG GTGCAGAAGA AGACCTTCAC CAAGTGGGTG
    AACCTGCACT TGGCGTGCGT CACCTGCCGC ATCTCCGACC TCTACCTGGA CCTCCGGGAC
    GGGCGGATGC TCATCAAGCT GCTGGAGGTG CTGTCCAAAG AGATGCTGCC CAAGCCCACC
    AAGGGCCGGA TGCGCATCCA CTGCCTGGAG AACGTGGACA AAGCACTGCA GTTCCTGAAG
    GAGCAGCGAG TGCACCTGGA GAACATGGGC TCCCACGACA TCGTGGATGG CAACCACCGC
    CTCATCCTCG GCCTCATCTG GACCATCATC CTCCGCTTCC AGGTGCAAGG CGTCATCGAG
    GATATGGAGG AGGATACAGA GACACGCTCG CCCAGGGAAG CGCTGCTGCT CTGGTGCCAA
    ATGAAGACGA AAGGCTACCC TCACGTGAAT GTCACCAACT TCACCTCCAG CTGGAAGGAT
    GGACTGGCCT TCAACGCCCT CATCCACAAG CACAGGCCTG AGCTGTTTGA CTTCAAAACT
    CTGACCAAGT CCAACGCCCG GCACAACCTG GAGCATGCCT TCAGCATGGC CGAGAGGCAC
    CTGGGCATCA CCCCGCTCCT CGACCCTGAA GATGTGTTCA CGGAGAACCC CGATGAGAAG
    TCCATCATCA CCTACGTGGT GGCCTTCTAC CACTACTTCT CCGAGATGAA GAAACTGGAG
    GTGAAGGGCA GGCGGCTGGG CAAGGTCATT GAGCACGCCA AAGAGACCAA GCGGATGATC
    GAGGGCTACG GGGGCCTGGC CTCCAACCTG CTCACCTGGA TCGAGCAGAC CATCATCTCC
    CTCAACAGCC GCAGCTTTGC CAACTCGCTG GCCGGTGTGC AGCACCAGCT GCAGACCTTC
    AGCACCTACC GCACGGTGGA GAAGCCCCCC AAGTTTCAGG AGAAGGGCAA CCTGGAGGTG
    CTGCTCTTCA CCATCCAGTC TCGGATGCGA GCCAACAACC AGCGTGTCTA CACACCACAA
    GAGGGGCGTC TGGTCTGTGA CATCAACCGG GCATGGGAGA AGCTGGAGAA AGCAGAGCAT
    GAGCGGGAGC TGGCACTGCG CAATGAGCTC ATCCGGCAGG AGAAGCTGGA GCAGCTGGCA
    CGGCGCTTTG ACCGCAAAGC AGCCATGCGG GAGGCCTGGC TGAGTGAGAA CCAACGCCTG
    GTGGCACAGC AGGATAACTT TGGCCACGAC CTGACGGCGG TGGAGGCGGC CAAGAAGAAA
    CACGAGGCCA TTGAGACGGA CACAGCTGCC TACATGGAAC GGGTGCAGGC CATTGATGCA
    GTGGCGAAGG AGCTGGAGAG GGAGGGCTAC CACGACATCA AACGCATCAG GGGGCGCAAG
    GACAACATCC TGCAGCACTG GGAGTACCTC CAGGAGCTGC TGCGCAACCG GCGCCAGCGC
    CTTGAGATGA ACCTCACCCT GCAGCACCTC TTCCAGGAGA TGCTGCACAG CATCAACTGG
    ATGGATGAGG TCAAGGTGCA GCTGGCCTCA TCCAAATCTG GGAAGCACCT CCTTGAGGTG
    GAGGAGCTTC TGGAGACCCA CCGGCTACTG GAAAGTGACA TGGCCCTGCA GGCAGAGAAG
    GTGCGGGCCG TCAGCACTGC TGCCCTCCGA TTTGCCGACG CTGAGGGCTA CCGCCCCTGC
    GACCCCAGAG TGATCCGAGA CCGCGTGAAC CACCTGGAGA TGTGCCGGCG GGAGCTGCAG
    GCACTGGCGG CGAGGCGTAA AGCCCTGCTG GAGCAGTCCC GGGCTGTCTG GCAGTGTCTG
    CAGGAGCTGG ACGAGGCAGA GAGCTGGATC AAGGAGCAGG AGCAGATCTA CTCAGCGCTG
    GACTACGGCA AAGACCTGGC GGGCGTGCTG CTGCTGCGAC GCCGTCACAC CGCGCTGGAG
    GCTGAGCTGG AGGCACGGGG AGCCCGGCTG GACAAGGCGC TGGTGATGGC TGAGCAGCTG
    GCAGCAGCAG GGCGTGAGGC CGGGCGGCTG CGGGACCGGG CGGCTGCCGT GCGGGTGCTG
    TGGGACCAGC TGCAGGAGCT GGTGGCCTTC CGCCGCCGCG GGCTGCGGGA TGCCGAGGGC
    TTCTTCCAGT TCCAGGCAGA GGCTGAAGAG CTGGCAGAGG TTCTTACGGA GGCTCAGCAG
    CGGGCGGCCA GCGAGGAGCT GGGTCACGAC GAGGTCCACA CGCAGGCACT GCTGCGGGAG
    CACCAGGAGC TGCTGGAGGA GCTGGCGGCT GCCCAGCAGC TCCTGGAGCG CTTGGGTCAC
    CAAGCAGAGG GCTTCCCACC AGAGCTGCGG GCCGGACCCG AGGCGCACAA CCGGCTGGCA
    GCGCTGCGGG AGCTGCACCA CAAGGTGAGC ACGCTGGCCG AGACGCGTGG CCGAAGGCTG
    CGGGATGCCC TCAACCTCTA CACTGTTTTT GGAGAGAGCG AAGCCTGCCA GCTCTGGATG
    GGCGCCAAGG AGCGGTGGCT GGAGAAGCTG GAGGTGCCCA ACAAGCTGGA GGACCTGGAC
    GTGGTGAAGC ACAGGCTGGA TGGGCTGGAG CAGGAGATGG TTGGCGTGGC TTCTCAAATC
    GACGCTGTCA ACCGCTCAGC TGACGGTCTG CTGGAGAGCG GCCACCCACG CAGCCCACAG
    GTCCGGCAGT GCCAGCAGCA GCTCAATGAG CGGTGGGACC GTTTTCGGGA GCTGGTGTCC
    CAGTGGCGTG CAGCAGTGGG CTCGGCGCTC AACCTGCTCA GCTTCCAACT GGAGTGCGAG
    GAGACGCGCG CCTGGCTGCT GAGCAAGACA CGGGTGGTGG AGTCCACAAA GGAGCTCGGC
    CGTGATCTGG CTGGAGTCCT GGCCACCCAG CGCAAGCTGT ATGGCATTGA ACGTGAGCTG
    GCAGCAACCG AGAGCCGCCT GGGTGCCCTG CGCCCGCAAG CCGCCCTCCT GGCCCAGGAG
    CGCCCTGAGT TGGCCGAGGA CACGGCACGG CGTCTGGCGG GGGCACAGGA TGCCATGGAC
    GGGCTGCAGG GCGCCCTGCG GGACCGAGCG GCTGCGCTGG GAGAAGTGGG GCAGCTGCAG
    AGCTTCCTGC AGGACCTGGA TGACTTCCAA GCGTGGCTCT TTGGGGCGCA GAAAGCCGTG
    GCAGGCACCG AAGAGGTGCC GACTTCGGTG GGTGAGGCAG AAGAGCTGCT GCGGAAGCAT
    GCGGCTGCCC TCGAGGATGC CGAGGGGCAC ACAGCTGCCT TCACGGCGCT GCTGGAGGCA
    GGCGAGCGGG TGACAAGCAA CCAGGAGGAC CCCGAGTATG AGCAGCTGCG GGAGAGGCTG
    CGGGGCGTGG AGGCGGGCTG GGGAGCCCTG CACAAGATGT GGGATGCCCG GCAGCGCTTC
    CTTGCCCAGT GCTTGGGATT CCAGGAGTTC CTGCGTGACG CCAAACAGGC TGAGATTCTC
    CTCGCCAACC AGGAGTACAC GCTGGCCCAC CTGGAGCTAC CCCCTACACT GGAGGGTTCT
    ACAGCTGCCC TGCGCCGCTT TGAGGATTTC CGTGCCTCCA TGGAGAGCAG CGCTGAGAAG
    GTCCCTAGCG TGGTGGCCGC TGGCTCCAAG CTTGTGGCAG AGGGCAACAT CTTCTCTGAG
    AAGATCAGTG AGAAGAACCA GGCCCTGCAG GAGCGGCACC AGGTGAATGT GGCCAAGGCA
    CAGGAGGCGG CGGGCTTGCT GCAGGACAAC CACAGGCTGC AGAGCTTCCT GCAGAGCTGC
    CGGGAGCTCG CTGCTTGGGT GGATGAGAAG ATGCTGACAG TGCAGGACGC GTCCTATGGA
    GGTGCACGTG GTTTGCATGG CAAGTGGCAG AAGCACCAAG CCTTTATGGC AGAGCTGGCA
    GCCAACGAGG CATGGCTGGA GAAGATCAAA GCGGAGGGCA TGGAGCTGGC GAGCTGCAAA
    CCACAGTACG GTGCAGTGGT GGGCCAGCGG CTGGACGAGC TGCAGGCGCT GTGGAATGGA
    CTGCACGACG CTGCCAAGGA GAAGGGCCAG CAGCTCTTTG AGGCCAACCG CACTGAGCTG
    TACGCCCAGA GCTACAGTGA CCTGGAGCGC TGGCTGGGGC AGGTGGAGGG CGAGCTGCAC
    ACCACTGAGC GTGCCAAGGA CCTCACCAGC GCCAACCTAC TGCTCAAGAA ACTGACGCGG
    CTGGAGGAGC AGGTGGCGGC ACGACAGAAG GAACTGATGG AGCTGAAAGC ACCACTTGCT
    GGGGTCGCAC CAGAGCCCGA TGCGCAGGAG GAGAAGCTCC AGCAGCGATT CCTTGACCTG
    CTGGAGCCAC TGAGGAAGAA GAGGAAGGAG CTGGAAACCT CCAAGGCGAT GTACCAGCTG
    GGGAGGGACC TGGAGGACGA GATGTTATGG GTGCAGGAGA GGATGCCATG GGCCAGGTCA
    ACTGAGAACG GCACCAACCT CCAGACCGTG CAGCGTTTGG CCAAGAGGAA TGAGACCCTA
    CAGAAGGAGC TGCAGGGCCA TGCCCCCCGC CTGGCCGAGG TGCTGGAACG TGGGGAGGCG
    GCTGCTGGGG GACCCTGCCC GGAGCTGGCG GAGAGGGCAC GGGAGCTGCA GGCGCAGTGG
    GAAGCGCTGA GGGAAGAAGT GGTCGCACGG CAGCAGCGGC TGCGGGAGGC CAGCGAGGCC
    CAGCAGTACT ACCTGGATGC TGATGAGGCC AAAGCGTGGG TCAGCGAGCA GGAGCTCTTC
    ATGGGAGATG AGGAGAAGCC GAAGGACGAG GAAAGTGGTT TGGTGATGCT GAAGAGACAC
    GTGAGGCAGC AGCGCTCCAT CGAGGACTAT GGGCAGACCA TCAAGGAGCT GGCGGGGAGG
    GCTCAGCAGC TGCTCTCTGC TGGCCACCCT GAGGGGGAGC AGATCATCCG GCTGCAGGGC
    CAGGTGGACA AGTATTACGC GGGGCTGAAG GAGGCGGCCG AGGAGCGCCG GCGGCGCTTG
    GAGAACATGT GCCACCTCTT CCAGCTGAAG CGTGAGGTGG AGGACCTGGA GCAGTGGATT
    GCTGAGCGTG ACGTGGCAGC CTCCTCCCAG GAGCTGGGGC AGGACCTGGA CCATGTCACG
    ATACTGAGGG ATAAGTTTCG GGAGTTTGCA CGGGAAACAG GCAGCGTAGG GCAGGAACGT
    GTGGACCACG TCAACCTGAC CATCGAGGAC CTCATCGACG CGGGGCACGC CGAGGCTGCC
    ACCATGGCTG AGTGGAAGGA CGGACTGAAC GAGAGCTGGG CTGACCTCCT GGAGCTGATT
    GACACACGCA TGCAGTTGCT GGCCGCCTCC TATGATCTGC GCAAGTACTT CTATGACGGC
    TCTGAGCTGC TGGCCCTCAT TGCTGCCCGC CACCAGGAGC TGCCCCAGGA CCTGGGCGAC
    GACTCGGGTT CGGTGGAGGC TTTCCACCGC ATGCACAGCG CCTTCGAGCG TGACCTGCAG
    CTGCTGGAGG GGCAGGTGCA GCGGTTTCGA GAGGTGGCAG CTCGCCTGCA AACCGCCTAT
    GCTGGGGAGA AGGCAGTCAG CATCCAGCAG CAGGAGCAGG AGGTGGCACG GGCGCTGCAG
    GCACTGCTGG AGGCATGCAG TGGGCGCCGG GCGCAGCTGG TGGACACAGC CGACAAGTTC
    CGCTTCTTCA GCATGGCCCG TGACCTGCTC TCCTGGATGG AGAGCACTGT GCGGCAGATC
    CAGACACAGG AGAAGCCCAG GGACGTTTCC TCGGTGGAGC TGCTGATGAA GTACCACCAA
    GGCATCCACG CCGAGATAGA TGCCCGCGAC AAGAGCTTTG ATGCCTGCAT TGAACTGGGC
    AAGAAGCTGC TGCAGCGCAA ACACCGGGAG TCTCCTGAGA TCAAGGCAAA GCTGATGGAG
    CTGCTGGACA AGAGGAAGGC CATGATGGAG ATGTGGCAGA AGCGCTGGGA CCGGCTGCGG
    CTGCTGTTGG AGGTGTGCCA GTTCTCCCGG GACGCCTCAG TGGCCGAGTC GTGGCTGATG
    GCACAGGAGC CCTACCTGGC CAGCAGCGAC TACGGGCAGA CGGTGGACGC GGTGGAGAAG
    TTGCTGAAAC GGCACGAAGC CTTTGAGAAG TCCACGGCCA CGTGGGAGGA GCGCATCGCT
    GCCCTGAGGA AGCTGACGAC GCTGGAGCTC ATGGGCGGGC GGACGCTGCG GGAGGAGGAC
    GTGGTGCACG ACGCTGCTCC CGAACGTCAG CTGGATCTGG ATGCAGAGCT GGAGGCAGGG
    TCTGAGGAGG AGGAGGAGAA GAGGAAGGGG GTGATCCCTC AGGAGGCCTC TCCGCCCGCT
    GTCGATGGAC CCCAGCCGCT GACAGTGCCC GAGGAGGAGC CCGTAGCACC GCGGGAGGAG
    GTGGCCACAT TGCCCGCCCA GCCTGCCCAC GTTCAGCTGG AGGGCTACCT GGGCCGCAAG
    CACGACCTGG AGGCGGCCAC CAAGCGTGCG TCCAACAGGT CGTGGAGCAC GCGGTACTGC
    GTGCTGCGGG GCGAGGAGCT TGCCTTCTTC AAGGACGCCA AGAGCCGGGC ACTGGGGGTG
    CCCTGCCAGG GTGAGGAGCC CCTGGGGCTG CGCAACGCAC GCTGCCAGGT GGCTGCTGGC
    TACAAGAAGA AGAAGCACGT CTTTGCGCTC AGGCTCAGCA ATGGCAGTGA GTGGCTCTTC
    CACGGCAAGG ATGAGGAGGA GATGCAGACG TGGCTGCAGG GGCTGCACGT GGCCATCACC
    TCCTGTCAGA ACCTGCCGGC CAAGGCGCGC AGCCTGCCCC CGCCCCACGC CCCCCCCGAG
    CCCCGGAGGG ACAAGGAGAA GCGCTTCAGC TTCTTCCCCA AGAAGAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa36100
    Clone ID Related Accession (Same CDS sequence) XM_015286287.1
    Accession Version XM_015286287.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6888bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product spectrin beta chain, erythrocytic isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455869.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGACCTCCG AGAATGACTA CGACCATCTG GAGCTGCAGC AGTCCTACAG CCACTGGGAT 
    GCCTCCGACG ATGAGTTGGA CAACGACAAC AGCTCAGCGC GGCTCTTTGA GCGCTCCCGC
    ATCAAAGCTC TGGCAGATGA GCGGGAGGCG GTGCAGAAGA AGACCTTCAC CAAGTGGGTG
    AACCTGCACT TGGCGTGCGT CACCTGCCGC ATCTCCGACC TCTACCTGGA CCTCCGGGAC
    GGGCGGATGC TCATCAAGCT GCTGGAGGTG CTGTCCAAAG AGATGCTGCC CAAGCCCACC
    AAGGGCCGGA TGCGCATCCA CTGCCTGGAG AACGTGGACA AAGCACTGCA GTTCCTGAAG
    GAGCAGCGAG TGCACCTGGA GAACATGGGC TCCCACGACA TCGTGGATGG CAACCACCGC
    CTCATCCTCG GCCTCATCTG GACCATCATC CTCCGCTTCC AGGTGCAAGG CGTCATCGAG
    GATATGGAGG AGGATACAGA GACACGCTCG CCCAGGGAAG CGCTGCTGCT CTGGTGCCAA
    ATGAAGACGA AAGGCTACCC TCACGTGAAT GTCACCAACT TCACCTCCAG CTGGAAGGAT
    GGACTGGCCT TCAACGCCCT CATCCACAAG CACAGGCCTG AGCTGTTTGA CTTCAAAACT
    CTGACCAAGT CCAACGCCCG GCACAACCTG GAGCATGCCT TCAGCATGGC CGAGAGGCAC
    CTGGGCATCA CCCCGCTCCT CGACCCTGAA GATGTGTTCA CGGAGAACCC CGATGAGAAG
    TCCATCATCA CCTACGTGGT GGCCTTCTAC CACTACTTCT CCGAGATGAA GAAACTGGAG
    GTGAAGGGCA GGCGGCTGGG CAAGGTCATT GAGCACGCCA AAGAGACCAA GCGGATGATC
    GAGGGCTACG GGGGCCTGGC CTCCAACCTG CTCACCTGGA TCGAGCAGAC CATCATCTCC
    CTCAACAGCC GCAGCTTTGC CAACTCGCTG GCCGGTGTGC AGCACCAGCT GCAGACCTTC
    AGCACCTACC GCACGGTGGA GAAGCCCCCC AAGTTTCAGG AGAAGGGCAA CCTGGAGGTG
    CTGCTCTTCA CCATCCAGTC TCGGATGCGA GCCAACAACC AGCGTGTCTA CACACCACAA
    GAGGGGCGTC TGGTCTGTGA CATCAACCGG GCATGGGAGA AGCTGGAGAA AGCAGAGCAT
    GAGCGGGAGC TGGCACTGCG CAATGAGCTC ATCCGGCAGG AGAAGCTGGA GCAGCTGGCA
    CGGCGCTTTG ACCGCAAAGC AGCCATGCGG GAGGCCTGGC TGAGTGAGAA CCAACGCCTG
    GTGGCACAGG ATAACTTTGG CCACGACCTG ACGGCGGTGG AGGCGGCCAA GAAGAAACAC
    GAGGCCATTG AGACGGACAC AGCTGCCTAC ATGGAACGGG TGCAGGCCAT TGATGCAGTG
    GCGAAGGAGC TGGAGAGGGA GGGCTACCAC GACATCAAAC GCATCAGGGG GCGCAAGGAC
    AACATCCTGC AGCACTGGGA GTACCTCCAG GAGCTGCTGC GCAACCGGCG CCAGCGCCTT
    GAGATGAACC TCACCCTGCA GCACCTCTTC CAGGAGATGC TGCACAGCAT CAACTGGATG
    GATGAGGTCA AGGTGCAGCT GGCCTCATCC AAATCTGGGA AGCACCTCCT TGAGGTGGAG
    GAGCTTCTGG AGACCCACCG GCTACTGGAA AGTGACATGG CCCTGCAGGC AGAGAAGGTG
    CGGGCCGTCA GCACTGCTGC CCTCCGATTT GCCGACGCTG AGGGCTACCG CCCCTGCGAC
    CCCAGAGTGA TCCGAGACCG CGTGAACCAC CTGGAGATGT GCCGGCGGGA GCTGCAGGCA
    CTGGCGGCGA GGCGTAAAGC CCTGCTGGAG CAGTCCCGGG CTGTCTGGCA GTGTCTGCAG
    GAGCTGGACG AGGCAGAGAG CTGGATCAAG GAGCAGGAGC AGATCTACTC AGCGCTGGAC
    TACGGCAAAG ACCTGGCGGG CGTGCTGCTG CTGCGACGCC GTCACACCGC GCTGGAGGCT
    GAGCTGGAGG CACGGGGAGC CCGGCTGGAC AAGGCGCTGG TGATGGCTGA GCAGCTGGCA
    GCAGCAGGGC GTGAGGCCGG GCGGCTGCGG GACCGGGCGG CTGCCGTGCG GGTGCTGTGG
    GACCAGCTGC AGGAGCTGGT GGCCTTCCGC CGCCGCGGGC TGCGGGATGC CGAGGGCTTC
    TTCCAGTTCC AGGCAGAGGC TGAAGAGCTG GCAGAGGTTC TTACGGAGGC TCAGCAGCGG
    GCGGCCAGCG AGGAGCTGGG TCACGACGAG GTCCACACGC AGGCACTGCT GCGGGAGCAC
    CAGGAGCTGC TGGAGGAGCT GGCGGCTGCC CAGCAGCTCC TGGAGCGCTT GGGTCACCAA
    GCAGAGGGCT TCCCACCAGA GCTGCGGGCC GGACCCGAGG CGCACAACCG GCTGGCAGCG
    CTGCGGGAGC TGCACCACAA GGTGAGCACG CTGGCCGAGA CGCGTGGCCG AAGGCTGCGG
    GATGCCCTCA ACCTCTACAC TGTTTTTGGA GAGAGCGAAG CCTGCCAGCT CTGGATGGGC
    GCCAAGGAGC GGTGGCTGGA GAAGCTGGAG GTGCCCAACA AGCTGGAGGA CCTGGACGTG
    GTGAAGCACA GGCTGGATGG GCTGGAGCAG GAGATGGTTG GCGTGGCTTC TCAAATCGAC
    GCTGTCAACC GCTCAGCTGA CGGTCTGCTG GAGAGCGGCC ACCCACGCAG CCCACAGGTC
    CGGCAGTGCC AGCAGCAGCT CAATGAGCGG TGGGACCGTT TTCGGGAGCT GGTGTCCCAG
    TGGCGTGCAG CAGTGGGCTC GGCGCTCAAC CTGCTCAGCT TCCAACTGGA GTGCGAGGAG
    ACGCGCGCCT GGCTGCTGAG CAAGACACGG GTGGTGGAGT CCACAAAGGA GCTCGGCCGT
    GATCTGGCTG GAGTCCTGGC CACCCAGCGC AAGCTGTATG GCATTGAACG TGAGCTGGCA
    GCAACCGAGA GCCGCCTGGG TGCCCTGCGC CCGCAAGCCG CCCTCCTGGC CCAGGAGCGC
    CCTGAGTTGG CCGAGGACAC GGCACGGCGT CTGGCGGGGG CACAGGATGC CATGGACGGG
    CTGCAGGGCG CCCTGCGGGA CCGAGCGGCT GCGCTGGGAG AAGTGGGGCA GCTGCAGAGC
    TTCCTGCAGG ACCTGGATGA CTTCCAAGCG TGGCTCTTTG GGGCGCAGAA AGCCGTGGCA
    GGCACCGAAG AGGTGCCGAC TTCGGTGGGT GAGGCAGAAG AGCTGCTGCG GAAGCATGCG
    GCTGCCCTCG AGGATGCCGA GGGGCACACA GCTGCCTTCA CGGCGCTGCT GGAGGCAGGC
    GAGCGGGTGA CAAGCAACCA GGAGGACCCC GAGTATGAGC AGCTGCGGGA GAGGCTGCGG
    GGCGTGGAGG CGGGCTGGGG AGCCCTGCAC AAGATGTGGG ATGCCCGGCA GCGCTTCCTT
    GCCCAGTGCT TGGGATTCCA GGAGTTCCTG CGTGACGCCA AACAGGCTGA GATTCTCCTC
    GCCAACCAGG AGTACACGCT GGCCCACCTG GAGCTACCCC CTACACTGGA GGGTTCTACA
    GCTGCCCTGC GCCGCTTTGA GGATTTCCGT GCCTCCATGG AGAGCAGCGC TGAGAAGGTC
    CCTAGCGTGG TGGCCGCTGG CTCCAAGCTT GTGGCAGAGG GCAACATCTT CTCTGAGAAG
    ATCAGTGAGA AGAACCAGGC CCTGCAGGAG CGGCACCAGG TGAATGTGGC CAAGGCACAG
    GAGGCGGCGG GCTTGCTGCA GGACAACCAC AGGCTGCAGA GCTTCCTGCA GAGCTGCCGG
    GAGCTCGCTG CTTGGGTGGA TGAGAAGATG CTGACAGTGC AGGACGCGTC CTATGGAGGT
    GCACGTGGTT TGCATGGCAA GTGGCAGAAG CACCAAGCCT TTATGGCAGA GCTGGCAGCC
    AACGAGGCAT GGCTGGAGAA GATCAAAGCG GAGGGCATGG AGCTGGCGAG CTGCAAACCA
    CAGTACGGTG CAGTGGTGGG CCAGCGGCTG GACGAGCTGC AGGCGCTGTG GAATGGACTG
    CACGACGCTG CCAAGGAGAA GGGCCAGCAG CTCTTTGAGG CCAACCGCAC TGAGCTGTAC
    GCCCAGAGCT ACAGTGACCT GGAGCGCTGG CTGGGGCAGG TGGAGGGCGA GCTGCACACC
    ACTGAGCGTG CCAAGGACCT CACCAGCGCC AACCTACTGC TCAAGAAACT GACGCGGCTG
    GAGGAGCAGG TGGCGGCACG ACAGAAGGAA CTGATGGAGC TGAAAGCACC ACTTGCTGGG
    GTCGCACCAG AGCCCGATGC GCAGGAGGAG AAGCTCCAGC AGCGATTCCT TGACCTGCTG
    GAGCCACTGA GGAAGAAGAG GAAGGAGCTG GAAACCTCCA AGGCGATGTA CCAGCTGGGG
    AGGGACCTGG AGGACGAGAT GTTATGGGTG CAGGAGAGGA TGCCATGGGC CAGGTCAACT
    GAGAACGGCA CCAACCTCCA GACCGTGCAG CGTTTGGCCA AGAGGAATGA GACCCTACAG
    AAGGAGCTGC AGGGCCATGC CCCCCGCCTG GCCGAGGTGC TGGAACGTGG GGAGGCGGCT
    GCTGGGGGAC CCTGCCCGGA GCTGGCGGAG AGGGCACGGG AGCTGCAGGC GCAGTGGGAA
    GCGCTGAGGG AAGAAGTGGT CGCACGGCAG CAGCGGCTGC GGGAGGCCAG CGAGGCCCAG
    CAGTACTACC TGGATGCTGA TGAGGCCAAA GCGTGGGTCA GCGAGCAGGA GCTCTTCATG
    GGAGATGAGG AGAAGCCGAA GGACGAGGAA AGTGGTTTGG TGATGCTGAA GAGACACGTG
    AGGCAGCAGC GCTCCATCGA GGACTATGGG CAGACCATCA AGGAGCTGGC GGGGAGGGCT
    CAGCAGCTGC TCTCTGCTGG CCACCCTGAG GGGGAGCAGA TCATCCGGCT GCAGGGCCAG
    GTGGACAAGT ATTACGCGGG GCTGAAGGAG GCGGCCGAGG AGCGCCGGCG GCGCTTGGAG
    AACATGTGCC ACCTCTTCCA GCTGAAGCGT GAGGTGGAGG ACCTGGAGCA GTGGATTGCT
    GAGCGTGACG TGGCAGCCTC CTCCCAGGAG CTGGGGCAGG ACCTGGACCA TGTCACGATA
    CTGAGGGATA AGTTTCGGGA GTTTGCACGG GAAACAGGCA GCGTAGGGCA GGAACGTGTG
    GACCACGTCA ACCTGACCAT CGAGGACCTC ATCGACGCGG GGCACGCCGA GGCTGCCACC
    ATGGCTGAGT GGAAGGACGG ACTGAACGAG AGCTGGGCTG ACCTCCTGGA GCTGATTGAC
    ACACGCATGC AGTTGCTGGC CGCCTCCTAT GATCTGCGCA AGTACTTCTA TGACGGCTCT
    GAGCTGCTGG CCCTCATTGC TGCCCGCCAC CAGGAGCTGC CCCAGGACCT GGGCGACGAC
    TCGGGTTCGG TGGAGGCTTT CCACCGCATG CACAGCGCCT TCGAGCGTGA CCTGCAGCTG
    CTGGAGGGGC AGGTGCAGCG GTTTCGAGAG GTGGCAGCTC GCCTGCAAAC CGCCTATGCT
    GGGGAGAAGG CAGTCAGCAT CCAGCAGCAG GAGCAGGAGG TGGCACGGGC GCTGCAGGCA
    CTGCTGGAGG CATGCAGTGG GCGCCGGGCG CAGCTGGTGG ACACAGCCGA CAAGTTCCGC
    TTCTTCAGCA TGGCCCGTGA CCTGCTCTCC TGGATGGAGA GCACTGTGCG GCAGATCCAG
    ACACAGGAGA AGCCCAGGGA CGTTTCCTCG GTGGAGCTGC TGATGAAGTA CCACCAAGGC
    ATCCACGCCG AGATAGATGC CCGCGACAAG AGCTTTGATG CCTGCATTGA ACTGGGCAAG
    AAGCTGCTGC AGCGCAAACA CCGGGAGTCT CCTGAGATCA AGGCAAAGCT GATGGAGCTG
    CTGGACAAGA GGAAGGCCAT GATGGAGATG TGGCAGAAGC GCTGGGACCG GCTGCGGCTG
    CTGTTGGAGG TGTGCCAGTT CTCCCGGGAC GCCTCAGTGG CCGAGTCGTG GCTGATGGCA
    CAGGAGCCCT ACCTGGCCAG CAGCGACTAC GGGCAGACGG TGGACGCGGT GGAGAAGTTG
    CTGAAACGGC ACGAAGCCTT TGAGAAGTCC ACGGCCACGT GGGAGGAGCG CATCGCTGCC
    CTGAGGAAGC TGACGACGCT GGAGCTCATG GGCGGGCGGA CGCTGCGGGA GGAGGACGTG
    GTGCACGACG CTGCTCCCGA ACGTCAGCTG GATCTGGATG CAGAGCTGGA GGCAGGGTCT
    GAGGAGGAGG AGGAGAAGAG GAAGGGGGTG ATCCCTCAGG AGGCCTCTCC GCCCGCTGTC
    GATGGACCCC AGCCGCTGAC AGTGCCCGAG GAGGAGCCCG TAGCACCGCG GGAGGAGGTG
    GCCACATTGC CCGCCCAGCC TGCCCACGTT CAGCTGGAGG GCTACCTGGG CCGCAAGCAC
    GACCTGGAGG CGGCCACCAA GCGTGCGTCC AACAGGTCGT GGAGCACGCG GTACTGCGTG
    CTGCGGGGCG AGGAGCTTGC CTTCTTCAAG GACGCCAAGA GCCGGGCACT GGGGGTGCCC
    TGCCAGGGTG AGGAGCCCCT GGGGCTGCGC AACGCACGCT GCCAGGTGGC TGCTGGCTAC
    AAGAAGAAGA AGCACGTCTT TGCGCTCAGG CTCAGCAATG GCAGTGAGTG GCTCTTCCAC
    GGCAAGGATG AGGAGGAGAT GCAGACGTGG CTGCAGGGGC TGCACGTGGC CATCACCTCC
    TGTCAGAACC TGCCGGCCAA GGCGCGCAGC CTGCCCCCGC CCCACGCCCC CCCCGAGCCC
    CGGAGGGACA AGGAGAAGCG CTTCAGCTTC TTCCCCAAGA AGAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015141773.1
    CDS195..7082
    Translation

    Target ORF information:

    RefSeq Version XM_015286287.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spectrin, beta, erythrocytic (SPTB), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015286287.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGACCTCCG AGAATGACTA CGACCATCTG GAGCTGCAGC AGTCCTACAG CCACTGGGAT 
    GCCTCCGACG ATGAGTTGGA CAACGACAAC AGCTCAGCGC GGCTCTTTGA GCGCTCCCGC
    ATCAAAGCTC TGGCAGATGA GCGGGAGGCG GTGCAGAAGA AGACCTTCAC CAAGTGGGTG
    AACCTGCACT TGGCGTGCGT CACCTGCCGC ATCTCCGACC TCTACCTGGA CCTCCGGGAC
    GGGCGGATGC TCATCAAGCT GCTGGAGGTG CTGTCCAAAG AGATGCTGCC CAAGCCCACC
    AAGGGCCGGA TGCGCATCCA CTGCCTGGAG AACGTGGACA AAGCACTGCA GTTCCTGAAG
    GAGCAGCGAG TGCACCTGGA GAACATGGGC TCCCACGACA TCGTGGATGG CAACCACCGC
    CTCATCCTCG GCCTCATCTG GACCATCATC CTCCGCTTCC AGGTGCAAGG CGTCATCGAG
    GATATGGAGG AGGATACAGA GACACGCTCG CCCAGGGAAG CGCTGCTGCT CTGGTGCCAA
    ATGAAGACGA AAGGCTACCC TCACGTGAAT GTCACCAACT TCACCTCCAG CTGGAAGGAT
    GGACTGGCCT TCAACGCCCT CATCCACAAG CACAGGCCTG AGCTGTTTGA CTTCAAAACT
    CTGACCAAGT CCAACGCCCG GCACAACCTG GAGCATGCCT TCAGCATGGC CGAGAGGCAC
    CTGGGCATCA CCCCGCTCCT CGACCCTGAA GATGTGTTCA CGGAGAACCC CGATGAGAAG
    TCCATCATCA CCTACGTGGT GGCCTTCTAC CACTACTTCT CCGAGATGAA GAAACTGGAG
    GTGAAGGGCA GGCGGCTGGG CAAGGTCATT GAGCACGCCA AAGAGACCAA GCGGATGATC
    GAGGGCTACG GGGGCCTGGC CTCCAACCTG CTCACCTGGA TCGAGCAGAC CATCATCTCC
    CTCAACAGCC GCAGCTTTGC CAACTCGCTG GCCGGTGTGC AGCACCAGCT GCAGACCTTC
    AGCACCTACC GCACGGTGGA GAAGCCCCCC AAGTTTCAGG AGAAGGGCAA CCTGGAGGTG
    CTGCTCTTCA CCATCCAGTC TCGGATGCGA GCCAACAACC AGCGTGTCTA CACACCACAA
    GAGGGGCGTC TGGTCTGTGA CATCAACCGG GCATGGGAGA AGCTGGAGAA AGCAGAGCAT
    GAGCGGGAGC TGGCACTGCG CAATGAGCTC ATCCGGCAGG AGAAGCTGGA GCAGCTGGCA
    CGGCGCTTTG ACCGCAAAGC AGCCATGCGG GAGGCCTGGC TGAGTGAGAA CCAACGCCTG
    GTGGCACAGG ATAACTTTGG CCACGACCTG ACGGCGGTGG AGGCGGCCAA GAAGAAACAC
    GAGGCCATTG AGACGGACAC AGCTGCCTAC ATGGAACGGG TGCAGGCCAT TGATGCAGTG
    GCGAAGGAGC TGGAGAGGGA GGGCTACCAC GACATCAAAC GCATCAGGGG GCGCAAGGAC
    AACATCCTGC AGCACTGGGA GTACCTCCAG GAGCTGCTGC GCAACCGGCG CCAGCGCCTT
    GAGATGAACC TCACCCTGCA GCACCTCTTC CAGGAGATGC TGCACAGCAT CAACTGGATG
    GATGAGGTCA AGGTGCAGCT GGCCTCATCC AAATCTGGGA AGCACCTCCT TGAGGTGGAG
    GAGCTTCTGG AGACCCACCG GCTACTGGAA AGTGACATGG CCCTGCAGGC AGAGAAGGTG
    CGGGCCGTCA GCACTGCTGC CCTCCGATTT GCCGACGCTG AGGGCTACCG CCCCTGCGAC
    CCCAGAGTGA TCCGAGACCG CGTGAACCAC CTGGAGATGT GCCGGCGGGA GCTGCAGGCA
    CTGGCGGCGA GGCGTAAAGC CCTGCTGGAG CAGTCCCGGG CTGTCTGGCA GTGTCTGCAG
    GAGCTGGACG AGGCAGAGAG CTGGATCAAG GAGCAGGAGC AGATCTACTC AGCGCTGGAC
    TACGGCAAAG ACCTGGCGGG CGTGCTGCTG CTGCGACGCC GTCACACCGC GCTGGAGGCT
    GAGCTGGAGG CACGGGGAGC CCGGCTGGAC AAGGCGCTGG TGATGGCTGA GCAGCTGGCA
    GCAGCAGGGC GTGAGGCCGG GCGGCTGCGG GACCGGGCGG CTGCCGTGCG GGTGCTGTGG
    GACCAGCTGC AGGAGCTGGT GGCCTTCCGC CGCCGCGGGC TGCGGGATGC CGAGGGCTTC
    TTCCAGTTCC AGGCAGAGGC TGAAGAGCTG GCAGAGGTTC TTACGGAGGC TCAGCAGCGG
    GCGGCCAGCG AGGAGCTGGG TCACGACGAG GTCCACACGC AGGCACTGCT GCGGGAGCAC
    CAGGAGCTGC TGGAGGAGCT GGCGGCTGCC CAGCAGCTCC TGGAGCGCTT GGGTCACCAA
    GCAGAGGGCT TCCCACCAGA GCTGCGGGCC GGACCCGAGG CGCACAACCG GCTGGCAGCG
    CTGCGGGAGC TGCACCACAA GGTGAGCACG CTGGCCGAGA CGCGTGGCCG AAGGCTGCGG
    GATGCCCTCA ACCTCTACAC TGTTTTTGGA GAGAGCGAAG CCTGCCAGCT CTGGATGGGC
    GCCAAGGAGC GGTGGCTGGA GAAGCTGGAG GTGCCCAACA AGCTGGAGGA CCTGGACGTG
    GTGAAGCACA GGCTGGATGG GCTGGAGCAG GAGATGGTTG GCGTGGCTTC TCAAATCGAC
    GCTGTCAACC GCTCAGCTGA CGGTCTGCTG GAGAGCGGCC ACCCACGCAG CCCACAGGTC
    CGGCAGTGCC AGCAGCAGCT CAATGAGCGG TGGGACCGTT TTCGGGAGCT GGTGTCCCAG
    TGGCGTGCAG CAGTGGGCTC GGCGCTCAAC CTGCTCAGCT TCCAACTGGA GTGCGAGGAG
    ACGCGCGCCT GGCTGCTGAG CAAGACACGG GTGGTGGAGT CCACAAAGGA GCTCGGCCGT
    GATCTGGCTG GAGTCCTGGC CACCCAGCGC AAGCTGTATG GCATTGAACG TGAGCTGGCA
    GCAACCGAGA GCCGCCTGGG TGCCCTGCGC CCGCAAGCCG CCCTCCTGGC CCAGGAGCGC
    CCTGAGTTGG CCGAGGACAC GGCACGGCGT CTGGCGGGGG CACAGGATGC CATGGACGGG
    CTGCAGGGCG CCCTGCGGGA CCGAGCGGCT GCGCTGGGAG AAGTGGGGCA GCTGCAGAGC
    TTCCTGCAGG ACCTGGATGA CTTCCAAGCG TGGCTCTTTG GGGCGCAGAA AGCCGTGGCA
    GGCACCGAAG AGGTGCCGAC TTCGGTGGGT GAGGCAGAAG AGCTGCTGCG GAAGCATGCG
    GCTGCCCTCG AGGATGCCGA GGGGCACACA GCTGCCTTCA CGGCGCTGCT GGAGGCAGGC
    GAGCGGGTGA CAAGCAACCA GGAGGACCCC GAGTATGAGC AGCTGCGGGA GAGGCTGCGG
    GGCGTGGAGG CGGGCTGGGG AGCCCTGCAC AAGATGTGGG ATGCCCGGCA GCGCTTCCTT
    GCCCAGTGCT TGGGATTCCA GGAGTTCCTG CGTGACGCCA AACAGGCTGA GATTCTCCTC
    GCCAACCAGG AGTACACGCT GGCCCACCTG GAGCTACCCC CTACACTGGA GGGTTCTACA
    GCTGCCCTGC GCCGCTTTGA GGATTTCCGT GCCTCCATGG AGAGCAGCGC TGAGAAGGTC
    CCTAGCGTGG TGGCCGCTGG CTCCAAGCTT GTGGCAGAGG GCAACATCTT CTCTGAGAAG
    ATCAGTGAGA AGAACCAGGC CCTGCAGGAG CGGCACCAGG TGAATGTGGC CAAGGCACAG
    GAGGCGGCGG GCTTGCTGCA GGACAACCAC AGGCTGCAGA GCTTCCTGCA GAGCTGCCGG
    GAGCTCGCTG CTTGGGTGGA TGAGAAGATG CTGACAGTGC AGGACGCGTC CTATGGAGGT
    GCACGTGGTT TGCATGGCAA GTGGCAGAAG CACCAAGCCT TTATGGCAGA GCTGGCAGCC
    AACGAGGCAT GGCTGGAGAA GATCAAAGCG GAGGGCATGG AGCTGGCGAG CTGCAAACCA
    CAGTACGGTG CAGTGGTGGG CCAGCGGCTG GACGAGCTGC AGGCGCTGTG GAATGGACTG
    CACGACGCTG CCAAGGAGAA GGGCCAGCAG CTCTTTGAGG CCAACCGCAC TGAGCTGTAC
    GCCCAGAGCT ACAGTGACCT GGAGCGCTGG CTGGGGCAGG TGGAGGGCGA GCTGCACACC
    ACTGAGCGTG CCAAGGACCT CACCAGCGCC AACCTACTGC TCAAGAAACT GACGCGGCTG
    GAGGAGCAGG TGGCGGCACG ACAGAAGGAA CTGATGGAGC TGAAAGCACC ACTTGCTGGG
    GTCGCACCAG AGCCCGATGC GCAGGAGGAG AAGCTCCAGC AGCGATTCCT TGACCTGCTG
    GAGCCACTGA GGAAGAAGAG GAAGGAGCTG GAAACCTCCA AGGCGATGTA CCAGCTGGGG
    AGGGACCTGG AGGACGAGAT GTTATGGGTG CAGGAGAGGA TGCCATGGGC CAGGTCAACT
    GAGAACGGCA CCAACCTCCA GACCGTGCAG CGTTTGGCCA AGAGGAATGA GACCCTACAG
    AAGGAGCTGC AGGGCCATGC CCCCCGCCTG GCCGAGGTGC TGGAACGTGG GGAGGCGGCT
    GCTGGGGGAC CCTGCCCGGA GCTGGCGGAG AGGGCACGGG AGCTGCAGGC GCAGTGGGAA
    GCGCTGAGGG AAGAAGTGGT CGCACGGCAG CAGCGGCTGC GGGAGGCCAG CGAGGCCCAG
    CAGTACTACC TGGATGCTGA TGAGGCCAAA GCGTGGGTCA GCGAGCAGGA GCTCTTCATG
    GGAGATGAGG AGAAGCCGAA GGACGAGGAA AGTGGTTTGG TGATGCTGAA GAGACACGTG
    AGGCAGCAGC GCTCCATCGA GGACTATGGG CAGACCATCA AGGAGCTGGC GGGGAGGGCT
    CAGCAGCTGC TCTCTGCTGG CCACCCTGAG GGGGAGCAGA TCATCCGGCT GCAGGGCCAG
    GTGGACAAGT ATTACGCGGG GCTGAAGGAG GCGGCCGAGG AGCGCCGGCG GCGCTTGGAG
    AACATGTGCC ACCTCTTCCA GCTGAAGCGT GAGGTGGAGG ACCTGGAGCA GTGGATTGCT
    GAGCGTGACG TGGCAGCCTC CTCCCAGGAG CTGGGGCAGG ACCTGGACCA TGTCACGATA
    CTGAGGGATA AGTTTCGGGA GTTTGCACGG GAAACAGGCA GCGTAGGGCA GGAACGTGTG
    GACCACGTCA ACCTGACCAT CGAGGACCTC ATCGACGCGG GGCACGCCGA GGCTGCCACC
    ATGGCTGAGT GGAAGGACGG ACTGAACGAG AGCTGGGCTG ACCTCCTGGA GCTGATTGAC
    ACACGCATGC AGTTGCTGGC CGCCTCCTAT GATCTGCGCA AGTACTTCTA TGACGGCTCT
    GAGCTGCTGG CCCTCATTGC TGCCCGCCAC CAGGAGCTGC CCCAGGACCT GGGCGACGAC
    TCGGGTTCGG TGGAGGCTTT CCACCGCATG CACAGCGCCT TCGAGCGTGA CCTGCAGCTG
    CTGGAGGGGC AGGTGCAGCG GTTTCGAGAG GTGGCAGCTC GCCTGCAAAC CGCCTATGCT
    GGGGAGAAGG CAGTCAGCAT CCAGCAGCAG GAGCAGGAGG TGGCACGGGC GCTGCAGGCA
    CTGCTGGAGG CATGCAGTGG GCGCCGGGCG CAGCTGGTGG ACACAGCCGA CAAGTTCCGC
    TTCTTCAGCA TGGCCCGTGA CCTGCTCTCC TGGATGGAGA GCACTGTGCG GCAGATCCAG
    ACACAGGAGA AGCCCAGGGA CGTTTCCTCG GTGGAGCTGC TGATGAAGTA CCACCAAGGC
    ATCCACGCCG AGATAGATGC CCGCGACAAG AGCTTTGATG CCTGCATTGA ACTGGGCAAG
    AAGCTGCTGC AGCGCAAACA CCGGGAGTCT CCTGAGATCA AGGCAAAGCT GATGGAGCTG
    CTGGACAAGA GGAAGGCCAT GATGGAGATG TGGCAGAAGC GCTGGGACCG GCTGCGGCTG
    CTGTTGGAGG TGTGCCAGTT CTCCCGGGAC GCCTCAGTGG CCGAGTCGTG GCTGATGGCA
    CAGGAGCCCT ACCTGGCCAG CAGCGACTAC GGGCAGACGG TGGACGCGGT GGAGAAGTTG
    CTGAAACGGC ACGAAGCCTT TGAGAAGTCC ACGGCCACGT GGGAGGAGCG CATCGCTGCC
    CTGAGGAAGC TGACGACGCT GGAGCTCATG GGCGGGCGGA CGCTGCGGGA GGAGGACGTG
    GTGCACGACG CTGCTCCCGA ACGTCAGCTG GATCTGGATG CAGAGCTGGA GGCAGGGTCT
    GAGGAGGAGG AGGAGAAGAG GAAGGGGGTG ATCCCTCAGG AGGCCTCTCC GCCCGCTGTC
    GATGGACCCC AGCCGCTGAC AGTGCCCGAG GAGGAGCCCG TAGCACCGCG GGAGGAGGTG
    GCCACATTGC CCGCCCAGCC TGCCCACGTT CAGCTGGAGG GCTACCTGGG CCGCAAGCAC
    GACCTGGAGG CGGCCACCAA GCGTGCGTCC AACAGGTCGT GGAGCACGCG GTACTGCGTG
    CTGCGGGGCG AGGAGCTTGC CTTCTTCAAG GACGCCAAGA GCCGGGCACT GGGGGTGCCC
    TGCCAGGGTG AGGAGCCCCT GGGGCTGCGC AACGCACGCT GCCAGGTGGC TGCTGGCTAC
    AAGAAGAAGA AGCACGTCTT TGCGCTCAGG CTCAGCAATG GCAGTGAGTG GCTCTTCCAC
    GGCAAGGATG AGGAGGAGAT GCAGACGTGG CTGCAGGGGC TGCACGTGGC CATCACCTCC
    TGTCAGAACC TGCCGGCCAA GGCGCGCAGC CTGCCCCCGC CCCACGCCCC CCCCGAGCCC
    CGGAGGGACA AGGAGAAGCG CTTCAGCTTC TTCCCCAAGA AGAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.