COL4A4 cDNA ORF clone, Gallus gallus(chicken)

The following COL4A4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL4A4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa152836 XM_025153677.1
Latest version!
Gallus gallus collagen type IV alpha 4 chain (COL4A4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OGa39728 XM_015276987.2
Latest version!
Gallus gallus collagen type IV alpha 4 chain (COL4A4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OGa39728 XM_015276986.2
Latest version!
Gallus gallus collagen type IV alpha 4 chain (COL4A4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OGa39729 XM_015276988.1
Latest version!
Gallus gallus collagen, type IV, alpha 4 (COL4A4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
View Old Accession Versions >>
OGa39728 XM_015276987.1 Gallus gallus collagen, type IV, alpha 4 (COL4A4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OGa39728 XM_015276986.1 Gallus gallus collagen, type IV, alpha 4 (COL4A4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa152836
    Clone ID Related Accession (Same CDS sequence) XM_025153677.1
    Accession Version XM_025153677.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5028bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product collagen alpha-4(IV) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGCGTTAA CAAAGGATTC ATTTGAACGA ATTAAGTGGC TTATCAGAAC TGCCTGGTGG 
    CTGCTGATTG TTTTTTCAGC ACAAGAAATT GATGGGGGAG GATACGCATA TATTGACCCA
    TGTGGGGGAC AGGATTGTTC GGTTTGTCGG TGCTTTCCAG AAAAAGGATC AAGAGGTCAA
    CCTGGTGAGC TGGGAGCTCA AGGTCCAATT GGATCGTTAG GACCTACAGG ACCAGCTGGT
    CTGCCAGGGG AAAAAGGACA GAGAGGTGAG AATGGGCAGC CTGGGCCAGC TGGAGAAAAA
    GGTGATAAGG GCCCAACTGG AGTCCCAGGA TTTCCTGGTT TAGATGGTGT TCCTGGATTG
    CCAGGAAGTG AAGGACCCAG GGGCAAGCAC GGTGTAGATG GCTGCAATGG CTCAAGAGGA
    GATCCAGGAT TTCCAGGCGA AAATGGATAT ATTGGACCAA GAGGTCCTTA CGGAAACCCA
    GGGCAAAAAG GAGAAAAAGG AAATTCAGTG TATGTTTCGT ATTATGGAAA AGGACCCCCA
    GGAGACAGAG GTGATCCTGG CCCACCTGGT ATGCCCGGAC CTAGAGGATC GGCAGGTACA
    ACAGGCCCAC CTGGATACCC AGGCCAACCA GGCATGCCCG GTATTCCAGG TTACCCTGGT
    TTGCCAGGTG AACAGGGAAA TCCAGGTATT GGAGTGGATG GACAAAAGGG GGAGCCGGGT
    GATGTTGGAC TTCCCGGGCC ACCTGGGTCA CCACTGTTAG TTGGACCTCC TGGAGCTCAG
    CTGTTCAAAG GAGAAAAGGG CCAAAAAGGA CTACCTGGGA TAACGGGATA CAGAGGACCA
    CATGGACCTA AAGGAATACC TGGGAAAGGA GAAAAGGGTG AAAAAGGCAT TCCTGGATTC
    CCTGGATTGC GGGGTAGCCC TGGTTCTCAT GGACCTGCAG GCTTTCCTGG AATAAAGGGA
    GAAATGGGTG TTGCTGGTTT TCCTGGGAAA CCTGGCTATC CAGGCATCAA GGGGGATCCT
    GGAGAAAAAG GGCTCCCCGG TCCTCCTGGA ACTCCACTGC TTCCTATGAA AGGCCCACCA
    GGAGATCCTG GATTTCCAGG TCCTGCGGGT GACATGGGGT CAGTTGGACC TTCTGGCCCT
    GTGGGTATGC TAGGAAGACC AGGAGACGAT GGAACAAGTC TGCCTGGCCT GCCAGGAATA
    AGTGGGGCAC CAGGACCTCA AGGTTTTCAG GGTGACCCTG GTTTGCCTGG AACAGGTGAA
    GCAATTCCAG GAAGACCTGG ATTTCCTGGA CCACCTGGAC TACCAGGACA GCCAGGAAGA
    CAAGGCTTAC CTGGACTACC TAGTGTAATT TGTACTGAGA GAGGGATTCC TGGGGAACCT
    GGAGCAAAAG GTCAGATAGG CCTTCCGGGT AGAAAAGGTG AAAAAGGAGA AAAAGGAAAT
    CAAGGCCTCT GCTCATGTAG TGCTGGTCCT CCTGGTCCCC GTGGTATACA AGGACCTTCA
    GGTACTCAAG GGAAGAAAGG ACAAATGGGT TACCCTGGAA GACGTGGAGA AAAGGGTGAT
    TCAGGCTTAT CTGGTGCAGT GGGATCACCA GGACTCCCTG GGACACCTGG TTCTGGAGGA
    CAACATGGTG AAAAAGGAGA GAAGGGTGAC CAGGGAAGAG TTTCAATAAA AGGAATAAAA
    GGTGAAAGAG GTCCTACTGG GGTTCCAGGA TTTCCAGGCC AAAAAGGTAA TGATGGCAGA
    GATGGGGATT TAGGATTGCC TGGGGAAAAA GGAGATGAGG GTGATTCTGG AGTACCAATA
    CCAGGTGATA AAGGATTTCC TGGAGTTCCA GGACTCCCTG GAGTAAAAGG ACCAATGGGA
    TTGCCTGGTT TGGGGTTTCC TGGCCCTCCG GGAGTTAGAG GTACCCCTGG AGACTTTGGT
    GATGATGGTA ACATTGGTCT GCCTGGTCCA AAGGGTCAGA AAGGTGACAC TATCTGTATT
    ACATCACCGT ACCCTGGAAA CCCAGGTCCC CCAGGTTTTA AAGGTGTTCA AGGACCAAAG
    GGTTTAAAAG GACTCCCTGG TCTTCCTGGA CGAAGTGGCT TTGATGGGCC AAAAGGCCAG
    CAAGGCAGGC CAGGAGCAGG AATCCCTGGA CCAGAAGGCT TCCGAGGCAC AGCTGGTGAT
    CCTGGTGATG AAGGTGAAAG AGGATCTACC ATTGATGGTA AAAATGGTGC TCCAGGTCCA
    CCTGGCATAG ATGGCCAGAA GGGTGTTTCA GGTGATACTA CATATGGCCC TCCAGGATTC
    CCAGGCAACA GGGGACTGCC AGGACCACCT GGTGCACAAG GAGCAAGGGG TGATCCAGGC
    ATTCCTGGGC TTCAAGGGCA TCCTGGGACC CCAGGATTTC CAGGTGCTAA AGGTTTTAGA
    GGTCCAGAAG GTGATACAGG AGCGCCAGGC TGTCCAGGCA CCCCTGGTCC ACCATGTGAC
    ACACAATTAC CAGGGCCACC TGGACTCAGA GGTGTCATAG GCCTGCCAGG TTCTCAAGGC
    TTACCAGGCT TTAAAGGTCA GAAAGGAGAC AGGGGCCTAG CTGGGCCACC TGGAATCAAG
    GGTCTCAAGG GCTCTCCTGG CTCACAGGGC CCCCCAGGTC CTCCAGGGTC CCGAGGACTT
    CCAGGACTAC CAGGCAATAA AGGACAACCT GGCTTTCCAG GTCAAACAGG AAGCAAAGGG
    ATTCAAGGAC CCCAAGGATT CCCTGGACTC CCAGGAACAC AAGGCCAAAT GGGAATTCCT
    GGTGTAAAAG GGGAAGAAGG AAACATGGGT CCACCTGGGC CTAGTGGAGA ATGTGGGGAT
    ATGGGAATGA AAGGTGAAAG AGGGCTTCCA GGAGGTGGTG GCAGCATTAA CATTCGGCTT
    GAGAAAGGAC AAAAAGGAGA ACCAGGGTTT CCTGGTGAGA ATGGATTTAG AGGTGAAAGA
    GGTGAAAAAG GAAATACAGG TTTCAGAGGT GCCCCAGGAT TTCCAGGTAA CAACGGTGCC
    CCAGGACTTC CAGGTGACCA TGGTGACATT GGACTGATGG GATTTCCAGG ATCACGTGGT
    TTCCCAGGAC CAAAGGGCTC TAAGGGAGTT ACAGGTTTCA AAGGACTACC AGGTGACCAG
    GGTGATGTTG GCTCACCAGG AATGCCTGGA AACCCAGGAC TGACAGGCCC CAAAGGACCT
    AAAGGAAAGA GAGGTGACCC AACTCTTCTG CTTGGTACAC AAGGTCAGAA AGGGCCTCCT
    GGAGATCTAG GATTACCAGG ATTCTGTGGA TTCCCAGGTG AGAAAGGTTC ACCAGGTATC
    CGAGGGCAAC CTGGAAGACC AGGATCCAAG GGCGAGCCTG GATACCCAGG AATACCTGGA
    TTTCCAGGAG CTACAGGTCC TCCAGGATTG CCAGGAGAAC CTGGAGAAAA AGGGAAACAT
    GGAATTTTGG GACCTCCAGG GCTGCTAGGA TTAACTGGAT CACCAGGTAG AAAAGGTCTT
    CCTGGACTGC CTGGACTGGA TGGCTTGGAC GGACTAAAAG GTCAAAAAGG TTCTGCAGGA
    GCTCCAGGTC AAAGTGAAAC AGGGCGCCCA GGATACTCTG GAGAACCTGG ACCAAAAGGA
    GAGAGAGGTG AACCTGGATG GCCTGGTATT TCCATTCCAG GCACCCCTGG AGAAAGAGGA
    TTCCCAGGAT TCCCAGGTAG AAGAGGACCT GTTGGACCCA TTGGACCTAG GGGACCATCT
    CCCAGTAGTG CTCCTCCTGG TCTTCCAGGT GATCAAGGAC CACCTGGTTT AGATGGCATC
    AGAGGTCAGC CTGGGAATCC TGGTCCTCCT GGAGAGACTG TCTTTGTACG AGGAGATCCA
    GGTGACACCG GTATTCGAGG GGCACCAGGC ATTCCTGGAC ACCGAGGGCT ACAAGGAGCC
    AGAGGTCTGC CAGGAAATCA AGGAAGAGAA GGTCCAAAGG GTCCAATGGG AATCCATGGC
    TTACAGGGTC CACCTGGTGC TGTAGGACAA CCAGGAGACC AAGGTTTTCA AGGAAAACCT
    GGTCCTAAAG GTCCCACAGG TGAGCCTGGT GAACCAGGAA AAACAGATGA CTCATGTCCT
    GCAATCCCAG GGCCTCCAGG GGAAGCAGGA CATAGAGGAG ATGATGGTTC TATAGGATTA
    CCAGGGCCAA TAGGTCACCC TGGACCCCAA GGAAGGAAAG GTGAAGAAGG GTCATGTGGC
    CTGCCAGGTC AAGATGGCTT ACCAGGAGCA CCTGGACCAC CAGGTGACCA AGGGCATGTG
    GGAGAACAAG GATATGCTGG ACCACAAGGT CCACCTGGCC AGACTGGTAT CCCAGGACCA
    CCAGGGCCCC ATGTTAGATC TTCAAGTGGA TTCTTGCTCG TCCTTCATAG TCAGTCAGAC
    AGAGAACCAC TTTGTCCACA GGGGATGCCA AAATTATGGA CTGGCTATAG TCTGCTGTAC
    CTGGAAGGAC AAGAGAAAGC TCATAATCAA GATCTTGGTC TGGCAGGATC TTGTCTTCCT
    GTATTTAATA CCATGCCCTT TGCTTACTGT AACATCAACC AAGTTTGTTA CTATGCCAGC
    CGAAATGATA AGTCCTACTG GTTGTCAAGT GCCGCTCCCT TACCAATGAC ACCACTGTCT
    GAGGAAGAAA TACAACCATA TATAAGCAGA TGTGCTGTAT GTGAGGCCCC AGCCCAAGCA
    GTAGCAGTTC ACAGCCAAGA TCAGTCAATT CCTCCCTGCC CAATGAACTG GAGAAGTCTT
    TGGATAGGAT ATTCTTTTCT GATGCACACT GGATCTGGTG ATCAGGGAGG TGGGCAGTCC
    CTTATGTCAC CAGGAAGCTG CTTAGAAGAC TTCAGATCAG CACCGTTCAT TGAATGCCAG
    GGTCAGCGGG GAACTTGTCA GTTCTTTGCT AATGAATACA GCTTTTGGCT GACAACAGTG
    ATGCCTGACT TGCAGTTTGC ATCAGCCCCC TTGTCAGGAA CTCTTAAAGA AGGACAAGAG
    CAGAGGAAAA AAATCAGTAG ATGCCAGGTA TGCCTGAAGC ATGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025009445.1
    CDS276..5303
    Translation

    Target ORF information:

    RefSeq Version XM_025153677.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus collagen type IV alpha 4 chain (COL4A4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025153677.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGCGTTAA CAAAGGATTC ATTTGAACGA ATTAAGTGGC TTATCAGAAC TGCCTGGTGG 
    CTGCTGATTG TTTTTTCAGC ACAAGAAATT GATGGGGGAG GATACGCATA TATTGACCCA
    TGTGGGGGAC AGGATTGTTC GGTTTGTCGG TGCTTTCCAG AAAAAGGATC AAGAGGTCAA
    CCTGGTGAGC TGGGAGCTCA AGGTCCAATT GGATCGTTAG GACCTACAGG ACCAGCTGGT
    CTGCCAGGGG AAAAAGGACA GAGAGGTGAG AATGGGCAGC CTGGGCCAGC TGGAGAAAAA
    GGTGATAAGG GCCCAACTGG AGTCCCAGGA TTTCCTGGTT TAGATGGTGT TCCTGGATTG
    CCAGGAAGTG AAGGACCCAG GGGCAAGCAC GGTGTAGATG GCTGCAATGG CTCAAGAGGA
    GATCCAGGAT TTCCAGGCGA AAATGGATAT ATTGGACCAA GAGGTCCTTA CGGAAACCCA
    GGGCAAAAAG GAGAAAAAGG AAATTCAGTG TATGTTTCGT ATTATGGAAA AGGACCCCCA
    GGAGACAGAG GTGATCCTGG CCCACCTGGT ATGCCCGGAC CTAGAGGATC GGCAGGTACA
    ACAGGCCCAC CTGGATACCC AGGCCAACCA GGCATGCCCG GTATTCCAGG TTACCCTGGT
    TTGCCAGGTG AACAGGGAAA TCCAGGTATT GGAGTGGATG GACAAAAGGG GGAGCCGGGT
    GATGTTGGAC TTCCCGGGCC ACCTGGGTCA CCACTGTTAG TTGGACCTCC TGGAGCTCAG
    CTGTTCAAAG GAGAAAAGGG CCAAAAAGGA CTACCTGGGA TAACGGGATA CAGAGGACCA
    CATGGACCTA AAGGAATACC TGGGAAAGGA GAAAAGGGTG AAAAAGGCAT TCCTGGATTC
    CCTGGATTGC GGGGTAGCCC TGGTTCTCAT GGACCTGCAG GCTTTCCTGG AATAAAGGGA
    GAAATGGGTG TTGCTGGTTT TCCTGGGAAA CCTGGCTATC CAGGCATCAA GGGGGATCCT
    GGAGAAAAAG GGCTCCCCGG TCCTCCTGGA ACTCCACTGC TTCCTATGAA AGGCCCACCA
    GGAGATCCTG GATTTCCAGG TCCTGCGGGT GACATGGGGT CAGTTGGACC TTCTGGCCCT
    GTGGGTATGC TAGGAAGACC AGGAGACGAT GGAACAAGTC TGCCTGGCCT GCCAGGAATA
    AGTGGGGCAC CAGGACCTCA AGGTTTTCAG GGTGACCCTG GTTTGCCTGG AACAGGTGAA
    GCAATTCCAG GAAGACCTGG ATTTCCTGGA CCACCTGGAC TACCAGGACA GCCAGGAAGA
    CAAGGCTTAC CTGGACTACC TAGTGTAATT TGTACTGAGA GAGGGATTCC TGGGGAACCT
    GGAGCAAAAG GTCAGATAGG CCTTCCGGGT AGAAAAGGTG AAAAAGGAGA AAAAGGAAAT
    CAAGGCCTCT GCTCATGTAG TGCTGGTCCT CCTGGTCCCC GTGGTATACA AGGACCTTCA
    GGTACTCAAG GGAAGAAAGG ACAAATGGGT TACCCTGGAA GACGTGGAGA AAAGGGTGAT
    TCAGGCTTAT CTGGTGCAGT GGGATCACCA GGACTCCCTG GGACACCTGG TTCTGGAGGA
    CAACATGGTG AAAAAGGAGA GAAGGGTGAC CAGGGAAGAG TTTCAATAAA AGGAATAAAA
    GGTGAAAGAG GTCCTACTGG GGTTCCAGGA TTTCCAGGCC AAAAAGGTAA TGATGGCAGA
    GATGGGGATT TAGGATTGCC TGGGGAAAAA GGAGATGAGG GTGATTCTGG AGTACCAATA
    CCAGGTGATA AAGGATTTCC TGGAGTTCCA GGACTCCCTG GAGTAAAAGG ACCAATGGGA
    TTGCCTGGTT TGGGGTTTCC TGGCCCTCCG GGAGTTAGAG GTACCCCTGG AGACTTTGGT
    GATGATGGTA ACATTGGTCT GCCTGGTCCA AAGGGTCAGA AAGGTGACAC TATCTGTATT
    ACATCACCGT ACCCTGGAAA CCCAGGTCCC CCAGGTTTTA AAGGTGTTCA AGGACCAAAG
    GGTTTAAAAG GACTCCCTGG TCTTCCTGGA CGAAGTGGCT TTGATGGGCC AAAAGGCCAG
    CAAGGCAGGC CAGGAGCAGG AATCCCTGGA CCAGAAGGCT TCCGAGGCAC AGCTGGTGAT
    CCTGGTGATG AAGGTGAAAG AGGATCTACC ATTGATGGTA AAAATGGTGC TCCAGGTCCA
    CCTGGCATAG ATGGCCAGAA GGGTGTTTCA GGTGATACTA CATATGGCCC TCCAGGATTC
    CCAGGCAACA GGGGACTGCC AGGACCACCT GGTGCACAAG GAGCAAGGGG TGATCCAGGC
    ATTCCTGGGC TTCAAGGGCA TCCTGGGACC CCAGGATTTC CAGGTGCTAA AGGTTTTAGA
    GGTCCAGAAG GTGATACAGG AGCGCCAGGC TGTCCAGGCA CCCCTGGTCC ACCATGTGAC
    ACACAATTAC CAGGGCCACC TGGACTCAGA GGTGTCATAG GCCTGCCAGG TTCTCAAGGC
    TTACCAGGCT TTAAAGGTCA GAAAGGAGAC AGGGGCCTAG CTGGGCCACC TGGAATCAAG
    GGTCTCAAGG GCTCTCCTGG CTCACAGGGC CCCCCAGGTC CTCCAGGGTC CCGAGGACTT
    CCAGGACTAC CAGGCAATAA AGGACAACCT GGCTTTCCAG GTCAAACAGG AAGCAAAGGG
    ATTCAAGGAC CCCAAGGATT CCCTGGACTC CCAGGAACAC AAGGCCAAAT GGGAATTCCT
    GGTGTAAAAG GGGAAGAAGG AAACATGGGT CCACCTGGGC CTAGTGGAGA ATGTGGGGAT
    ATGGGAATGA AAGGTGAAAG AGGGCTTCCA GGAGGTGGTG GCAGCATTAA CATTCGGCTT
    GAGAAAGGAC AAAAAGGAGA ACCAGGGTTT CCTGGTGAGA ATGGATTTAG AGGTGAAAGA
    GGTGAAAAAG GAAATACAGG TTTCAGAGGT GCCCCAGGAT TTCCAGGTAA CAACGGTGCC
    CCAGGACTTC CAGGTGACCA TGGTGACATT GGACTGATGG GATTTCCAGG ATCACGTGGT
    TTCCCAGGAC CAAAGGGCTC TAAGGGAGTT ACAGGTTTCA AAGGACTACC AGGTGACCAG
    GGTGATGTTG GCTCACCAGG AATGCCTGGA AACCCAGGAC TGACAGGCCC CAAAGGACCT
    AAAGGAAAGA GAGGTGACCC AACTCTTCTG CTTGGTACAC AAGGTCAGAA AGGGCCTCCT
    GGAGATCTAG GATTACCAGG ATTCTGTGGA TTCCCAGGTG AGAAAGGTTC ACCAGGTATC
    CGAGGGCAAC CTGGAAGACC AGGATCCAAG GGCGAGCCTG GATACCCAGG AATACCTGGA
    TTTCCAGGAG CTACAGGTCC TCCAGGATTG CCAGGAGAAC CTGGAGAAAA AGGGAAACAT
    GGAATTTTGG GACCTCCAGG GCTGCTAGGA TTAACTGGAT CACCAGGTAG AAAAGGTCTT
    CCTGGACTGC CTGGACTGGA TGGCTTGGAC GGACTAAAAG GTCAAAAAGG TTCTGCAGGA
    GCTCCAGGTC AAAGTGAAAC AGGGCGCCCA GGATACTCTG GAGAACCTGG ACCAAAAGGA
    GAGAGAGGTG AACCTGGATG GCCTGGTATT TCCATTCCAG GCACCCCTGG AGAAAGAGGA
    TTCCCAGGAT TCCCAGGTAG AAGAGGACCT GTTGGACCCA TTGGACCTAG GGGACCATCT
    CCCAGTAGTG CTCCTCCTGG TCTTCCAGGT GATCAAGGAC CACCTGGTTT AGATGGCATC
    AGAGGTCAGC CTGGGAATCC TGGTCCTCCT GGAGAGACTG TCTTTGTACG AGGAGATCCA
    GGTGACACCG GTATTCGAGG GGCACCAGGC ATTCCTGGAC ACCGAGGGCT ACAAGGAGCC
    AGAGGTCTGC CAGGAAATCA AGGAAGAGAA GGTCCAAAGG GTCCAATGGG AATCCATGGC
    TTACAGGGTC CACCTGGTGC TGTAGGACAA CCAGGAGACC AAGGTTTTCA AGGAAAACCT
    GGTCCTAAAG GTCCCACAGG TGAGCCTGGT GAACCAGGAA AAACAGATGA CTCATGTCCT
    GCAATCCCAG GGCCTCCAGG GGAAGCAGGA CATAGAGGAG ATGATGGTTC TATAGGATTA
    CCAGGGCCAA TAGGTCACCC TGGACCCCAA GGAAGGAAAG GTGAAGAAGG GTCATGTGGC
    CTGCCAGGTC AAGATGGCTT ACCAGGAGCA CCTGGACCAC CAGGTGACCA AGGGCATGTG
    GGAGAACAAG GATATGCTGG ACCACAAGGT CCACCTGGCC AGACTGGTAT CCCAGGACCA
    CCAGGGCCCC ATGTTAGATC TTCAAGTGGA TTCTTGCTCG TCCTTCATAG TCAGTCAGAC
    AGAGAACCAC TTTGTCCACA GGGGATGCCA AAATTATGGA CTGGCTATAG TCTGCTGTAC
    CTGGAAGGAC AAGAGAAAGC TCATAATCAA GATCTTGGTC TGGCAGGATC TTGTCTTCCT
    GTATTTAATA CCATGCCCTT TGCTTACTGT AACATCAACC AAGTTTGTTA CTATGCCAGC
    CGAAATGATA AGTCCTACTG GTTGTCAAGT GCCGCTCCCT TACCAATGAC ACCACTGTCT
    GAGGAAGAAA TACAACCATA TATAAGCAGA TGTGCTGTAT GTGAGGCCCC AGCCCAAGCA
    GTAGCAGTTC ACAGCCAAGA TCAGTCAATT CCTCCCTGCC CAATGAACTG GAGAAGTCTT
    TGGATAGGAT ATTCTTTTCT GATGCACACT GGATCTGGTG ATCAGGGAGG TGGGCAGTCC
    CTTATGTCAC CAGGAAGCTG CTTAGAAGAC TTCAGATCAG CACCGTTCAT TGAATGCCAG
    GGTCAGCGGG GAACTTGTCA GTTCTTTGCT AATGAATACA GCTTTTGGCT GACAACAGTG
    ATGCCTGACT TGCAGTTTGC ATCAGCCCCC TTGTCAGGAA CTCTTAAAGA AGGACAAGAG
    CAGAGGAAAA AAATCAGTAG ATGCCAGGTA TGCCTGAAGC ATGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39728
    Clone ID Related Accession (Same CDS sequence) XM_015276986.1 , XM_015276987.1 , XM_015276987.2 , XM_015276986.2
    Accession Version XM_015276986.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5037bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product collagen alpha-4(IV) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471743.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGCGTTAA CAAAGGATTC ATTTGAACGA ATTAAGTGGC TTATCAGAAC TGCCTGGTGG 
    CTGCTGATTG TTTTTTCAGC ACAAGAAATT GATGGGGGAG GATACGCATA TATTGACCCA
    TGTGGGGGAC AGGATTGTTC GGTTTGTCGG TGCTTTCCAG AAAAAGGATC AAGAGGTCAA
    CCTGGTGAGC TGGGAGCTCA AGGTCCAATT GGATCGTTAG GACCTACAGG ACCAGCTGGT
    CTGCCAGGGG AAAAAGGACA GAGAGGTGAG AATGGGCAGC CTGGGCCAGC TGGAGAAAAA
    GGTGATAAGG GCCCAACTGG AGTCCCAGGA TTTCCTGGTT TAGATGGTGT TCCTGGATTG
    CCAGGAAGTG AAGGACCCAG GGGCAAGCAC GGTGTAGATG GCTGCAATGG CTCAAGAGGA
    GATCCAGGAT TTCCAGGCGA AAATGGATAT ATTGGACCAA GAGGTCCTTA CGGAAACCCA
    GGGCAAAAAG GAGAAAAAGG AAATTCAGTG TATGTTTCGT ATTATGGAAA AGGACCCCCA
    GGAGACAGAG GTGATCCTGG CCCACCTGGT ATGCCCGGAC CTAGAGGATC GGCAGGTACA
    ACAGGCCCAC CTGGATACCC AGGCCAACCA GGCATGCCCG GTATTCCAGG TTACCCTGGT
    TTGCCAGGTG AACAGGGAAA TCCAGGTATT GGAGTGGATG GACAAAAGGG GGAGCCGGGT
    GATGTTGGAC TTCCCGGGCC ACCTGGGTCA CCACTGTTAG TTGGACCTCC TGGAGCTCAG
    CTGTTCAAAG GAGAAAAGGG CCAAAAAGGA CTACCTGGGA TAACGGGATA CAGAGGACCA
    CATGGACCTA AAGGAATACC TGGGAAAGGA GAAAAGGGTG AAAAAGGCAT TCCTGGATTC
    CCTGGATTGC GGGGTAGCCC TGGTTCTCAT GGACCTGCAG GCTTTCCTGG AATAAAGGGA
    GAAATGGGTG TTGCTGGTTT TCCTGGGAAA CCTGGCTATC CAGGCATCAA GGGGGATCCT
    GGAGAAAAAG GGCTCCCCGG TCCTCCTGGA ACTCCACTGC TTCCTATGAA AGGCCCACCA
    GGAGATCCTG GATTTCCAGG TCCTGCGGGT GACATGGGGT CAGTTGGACC TTCTGGCCCT
    GTGGGTATGC TAGGAAGACC AGGAGACGAT GGAACAAGTC TGCCTGGCCT GCCAGGAATA
    AGTGGGGCAC CAGGACCTCA AGGTTTTCAG GGTGACCCTG GTTTGCCTGG AACAGGTGAA
    GCAATTCCAG GAAGACCTGG ATTTCCTGGA CCACCTGGAC TACCAGGACA GCCAGGAAGA
    CAAGGCTTAC CTGGACTACC TAGTGTAATT TGTACTGAGA GAGGGATTCC TGGGGAACCT
    GGAGCAAAAG GTCAGATAGG CCTTCCGGGT AGAAAAGGTG AAAAAGGAGA AAAAGGAAAT
    CAAGGCCTCT GCTCATGTAG TGCTGGTCCT CCTGGTCCCC GTGGTATACA AGGACCTTCA
    GGTACTCAAG GGAAGAAAGG ACAAATGGGT TACCCTGGAA GACGTGGAGA AAAGGGTGAT
    TCAGGCTTAT CTGGTGCAGT GGGATCACCA GGACTCCCTG GGACACCTGG TTCTGGAGGA
    CAACATGGTG AAAAAGGAGA GAAGGGTGAC CAGGGAAGAG TTTCAATAAA AGGAATAAAA
    GGTGAAAGAG GTCCTACTGG GGTTCCAGGA TTTCCAGGCC AAAAAGGTAA TGATGGCAGA
    GATGGGGATT TAGGATTGCC TGGGGAAAAA GGAGATGAGG GTGATTCTGG AGTACCAATA
    CCAGGTGATA AAGGATTTCC TGGAGTTCCA GGACTCCCTG GAGTAAAAGG ACCAATGGGA
    TTGCCTGGTT TGGGGTTTCC TGGCCCTCCG GGAGTTAGAG GTACCCCTGG AGACTTTGGT
    GATGATGGTA ACATTGGTCT GCCTGGTCCA AAGGGTCAGA AAGGTGACAC TATCTGTATT
    ACATCACCGT ACCCTGGAAA CCCAGGTCCC CCAGGTTTTA AAGGTGTTCA AGGACCAAAG
    GGTTTAAAAG GACTCCCTGG TCTTCCTGGA CGAAGTGGCT TTGATGGGCC AAAAGGCCAG
    CAAGGCAGGC CAGGAGCAGG AATCCCTGGA CCAGAAGGCT TCCGAGGCAC AGCTGGTGAT
    CCTGGTGATG AAGGTGAAAG AGGATCTACC ATTGATGGTA AAAATGGTGC TCCAGGTCCA
    CCTGGCATAG ATGGCCAGAA GGGTGTTTCA GGTGATACTA CATATGGCCC TCCAGGATTC
    CCAGGCAACA GGGGACTGCC AGGACCACCT GGTGCACAAG GAGCAAGGGG TGATCCAGGC
    ATTCCTGGGC TTCAAGGGCA TCCTGGGACC CCAGGATTTC CAGGTGCTAA AGGTTTTAGA
    GGTCCAGAAG GTGATACAGG AGCGCCAGGC TGTCCAGGCA CCCCTGGTCC ACCATGTGAC
    ACACAATTAC CAGGGCCACC TGGACTCAGA GGTGTCATAG GCCTGCCAGG TTCTCAAGGC
    TTACCAGGCT TTAAAGGTCA GAAAGGAGAC AGGGGCCTAG CTGGGCCACC TGGAATCAAG
    GGTCTCAAGG GCTCTCCTGG CTCACAGGGC CCCCCAGGTC CTCCAGGGTC CCGAGGACTT
    CCAGGACTAC CAGGCAATAA AGGACAACCT GGCTTTCCAG GTCAAACAGG AAGCAAAGGG
    ATTCAAGGAC CCCAAGGATT CCCTGGACTC CCAGGAACAC AAGGCCAAAT GGGAATTCCT
    GGTGTAAAAG GGGAAGAAGG AAACATGGGT CCACCTGGGC CTAGTGGAGA ATGTGGGGAT
    ATGGGAATGA AAGGTGAAAG AGGGCTTCCA GGAGGTGGTG GCAGCATTAA CATTCGGCTT
    GAGAAAGGAC AAAAAGGAGA ACCAGGGTTT CCTGGTGAGA ATGGATTTAG AGGTGAAAGA
    GGTGAAAAAG GAAATACAGG TTTCAGAGGT GCCCCAGGAT TTCCAGGTAA CAACGGTGCC
    CCAGGACTTC CAGGTGACCA TGGTGACATT GGACTGATGG GATTTCCAGG ATCACGTGGT
    TTCCCAGGAC CAAAGGGCTC TAAGGGAGTT ACAGGTTTCA AAGGACTACC AGGTGACCAG
    GGTGATGTTG GCTCACCAGG AATGCCTGGA AACCCAGGAC TGACAGGCCC CAAAGGACCT
    AAAGGAAAGA GAGGTGACCC AACTCTTCTG CTTGGTACAC AAGGTCAGAA AGGGCCTCCT
    GGAGATCTAG GATTACCAGG ATTCTGTGGA TTCCCAGGTG AGAAAGGTTC ACCAGGTATC
    CGAGGGCAAC CTGGAAGACC AGGATCCAAG GGCGAGCCTG GATACCCAGG AATACCTGGA
    TTTCCAGGAG CTACAGGTCC TCCAGGATTG CCAGGAGAAC CTGGAGAAAA AGGGAAACAT
    GGAATTTTGG GACCTCCAGG GCTGCTAGGA TTAACTGGAT CACCAGGTAG AAAAGGTCTT
    CCTGGACTGC CTGGACTGGA TGGCTTGGAC GGACTAAAAG GTCAAAAAGG TTCTGCAGGA
    GCTCCAGGTC AAAGTGAAAC AGGGCGCCCA GGATACTCTG GAGAACCTGG ACCAAAAGGA
    GAGAGAGGTG AACCTGGATG GCCTGGTATT TCCATTCCAG GCACCCCTGG AGAAAGAGGA
    TTCCCAGGAT TCCCAGGTAG AAGAGGACCT GTTGGACCCA TTGGACCTAG GGGACCATCT
    CCCAGTAGTG CTCCTCCTGG TCTTCCAGGT GATCAAGGAC CACCTGGTTT AGATGGCATC
    AGAGGTCAGC CTGGGAATCC TGGTCCTCCT GGAGAGACTG TCTTTGTACG AGGAGATCCA
    GGTGACACCG GTATTCGAGG GGCACCAGGC ATTCCTGGAC ACCGAGGGCT ACAAGGAGCC
    AGAGGTCTGC CAGGAAATCA AGGAAGAGAA GGTCCAAAGG GTCCAATGGG AATCCATGGC
    TTACAGGGTC CACCTGGTGC TGTAGGACAA CCAGGAGACC AAGGTTTTCA AGGAAAACCT
    GGTCCTAAAG GTCCCACAGG GTTCATAGGT GAGCCTGGTG AACCAGGAAA AACAGATGAC
    TCATGTCCTG CAATCCCAGG GCCTCCAGGG GAAGCAGGAC ATAGAGGAGA TGATGGTTCT
    ATAGGATTAC CAGGGCCAAT AGGTCACCCT GGACCCCAAG GAAGGAAAGG TGAAGAAGGG
    TCATGTGGCC TGCCAGGTCA AGATGGCTTA CCAGGAGCAC CTGGACCACC AGGTGACCAA
    GGGCATGTGG GAGAACAAGG ATATGCTGGA CCACAAGGTC CACCTGGCCA GACTGGTATC
    CCAGGACCAC CAGGGCCCCA TGTTAGATCT TCAAGTGGAT TCTTGCTCGT CCTTCATAGT
    CAGTCAGACA GAGAACCACT TTGTCCACAG GGGATGCCAA AATTATGGAC TGGCTATAGT
    CTGCTGTACC TGGAAGGACA AGAGAAAGCT CATAATCAAG ATCTTGGTCT GGCAGGATCT
    TGTCTTCCTG TATTTAATAC CATGCCCTTT GCTTACTGTA ACATCAACCA AGTTTGTTAC
    TATGCCAGCC GAAATGATAA GTCCTACTGG TTGTCAAGTG CCGCTCCCTT ACCAATGACA
    CCACTGTCTG AGGAAGAAAT ACAACCATAT ATAAGCAGAT GTGCTGTATG TGAGGCCCCA
    GCCCAAGCAG TAGCAGTTCA CAGCCAAGAT CAGTCAATTC CTCCCTGCCC AATGAACTGG
    AGAAGTCTTT GGATAGGATA TTCTTTTCTG ATGCACACTG GATCTGGTGA TCAGGGAGGT
    GGGCAGTCCC TTATGTCACC AGGAAGCTGC TTAGAAGACT TCAGATCAGC ACCGTTCATT
    GAATGCCAGG GTCAGCGGGG AACTTGTCAG TTCTTTGCTA ATGAATACAG CTTTTGGCTG
    ACAACAGTGA TGCCTGACTT GCAGTTTGCA TCAGCCCCCT TGTCAGGAAC TCTTAAAGAA
    GGACAAGAGC AGAGGAAAAA AATCAGTAGA TGCCAGGTAT GCCTGAAGCA TGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015132472.1
    CDS237..5273
    Translation

    Target ORF information:

    RefSeq Version XM_015276986.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus collagen, type IV, alpha 4 (COL4A4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015276986.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGCGTTAA CAAAGGATTC ATTTGAACGA ATTAAGTGGC TTATCAGAAC TGCCTGGTGG 
    CTGCTGATTG TTTTTTCAGC ACAAGAAATT GATGGGGGAG GATACGCATA TATTGACCCA
    TGTGGGGGAC AGGATTGTTC GGTTTGTCGG TGCTTTCCAG AAAAAGGATC AAGAGGTCAA
    CCTGGTGAGC TGGGAGCTCA AGGTCCAATT GGATCGTTAG GACCTACAGG ACCAGCTGGT
    CTGCCAGGGG AAAAAGGACA GAGAGGTGAG AATGGGCAGC CTGGGCCAGC TGGAGAAAAA
    GGTGATAAGG GCCCAACTGG AGTCCCAGGA TTTCCTGGTT TAGATGGTGT TCCTGGATTG
    CCAGGAAGTG AAGGACCCAG GGGCAAGCAC GGTGTAGATG GCTGCAATGG CTCAAGAGGA
    GATCCAGGAT TTCCAGGCGA AAATGGATAT ATTGGACCAA GAGGTCCTTA CGGAAACCCA
    GGGCAAAAAG GAGAAAAAGG AAATTCAGTG TATGTTTCGT ATTATGGAAA AGGACCCCCA
    GGAGACAGAG GTGATCCTGG CCCACCTGGT ATGCCCGGAC CTAGAGGATC GGCAGGTACA
    ACAGGCCCAC CTGGATACCC AGGCCAACCA GGCATGCCCG GTATTCCAGG TTACCCTGGT
    TTGCCAGGTG AACAGGGAAA TCCAGGTATT GGAGTGGATG GACAAAAGGG GGAGCCGGGT
    GATGTTGGAC TTCCCGGGCC ACCTGGGTCA CCACTGTTAG TTGGACCTCC TGGAGCTCAG
    CTGTTCAAAG GAGAAAAGGG CCAAAAAGGA CTACCTGGGA TAACGGGATA CAGAGGACCA
    CATGGACCTA AAGGAATACC TGGGAAAGGA GAAAAGGGTG AAAAAGGCAT TCCTGGATTC
    CCTGGATTGC GGGGTAGCCC TGGTTCTCAT GGACCTGCAG GCTTTCCTGG AATAAAGGGA
    GAAATGGGTG TTGCTGGTTT TCCTGGGAAA CCTGGCTATC CAGGCATCAA GGGGGATCCT
    GGAGAAAAAG GGCTCCCCGG TCCTCCTGGA ACTCCACTGC TTCCTATGAA AGGCCCACCA
    GGAGATCCTG GATTTCCAGG TCCTGCGGGT GACATGGGGT CAGTTGGACC TTCTGGCCCT
    GTGGGTATGC TAGGAAGACC AGGAGACGAT GGAACAAGTC TGCCTGGCCT GCCAGGAATA
    AGTGGGGCAC CAGGACCTCA AGGTTTTCAG GGTGACCCTG GTTTGCCTGG AACAGGTGAA
    GCAATTCCAG GAAGACCTGG ATTTCCTGGA CCACCTGGAC TACCAGGACA GCCAGGAAGA
    CAAGGCTTAC CTGGACTACC TAGTGTAATT TGTACTGAGA GAGGGATTCC TGGGGAACCT
    GGAGCAAAAG GTCAGATAGG CCTTCCGGGT AGAAAAGGTG AAAAAGGAGA AAAAGGAAAT
    CAAGGCCTCT GCTCATGTAG TGCTGGTCCT CCTGGTCCCC GTGGTATACA AGGACCTTCA
    GGTACTCAAG GGAAGAAAGG ACAAATGGGT TACCCTGGAA GACGTGGAGA AAAGGGTGAT
    TCAGGCTTAT CTGGTGCAGT GGGATCACCA GGACTCCCTG GGACACCTGG TTCTGGAGGA
    CAACATGGTG AAAAAGGAGA GAAGGGTGAC CAGGGAAGAG TTTCAATAAA AGGAATAAAA
    GGTGAAAGAG GTCCTACTGG GGTTCCAGGA TTTCCAGGCC AAAAAGGTAA TGATGGCAGA
    GATGGGGATT TAGGATTGCC TGGGGAAAAA GGAGATGAGG GTGATTCTGG AGTACCAATA
    CCAGGTGATA AAGGATTTCC TGGAGTTCCA GGACTCCCTG GAGTAAAAGG ACCAATGGGA
    TTGCCTGGTT TGGGGTTTCC TGGCCCTCCG GGAGTTAGAG GTACCCCTGG AGACTTTGGT
    GATGATGGTA ACATTGGTCT GCCTGGTCCA AAGGGTCAGA AAGGTGACAC TATCTGTATT
    ACATCACCGT ACCCTGGAAA CCCAGGTCCC CCAGGTTTTA AAGGTGTTCA AGGACCAAAG
    GGTTTAAAAG GACTCCCTGG TCTTCCTGGA CGAAGTGGCT TTGATGGGCC AAAAGGCCAG
    CAAGGCAGGC CAGGAGCAGG AATCCCTGGA CCAGAAGGCT TCCGAGGCAC AGCTGGTGAT
    CCTGGTGATG AAGGTGAAAG AGGATCTACC ATTGATGGTA AAAATGGTGC TCCAGGTCCA
    CCTGGCATAG ATGGCCAGAA GGGTGTTTCA GGTGATACTA CATATGGCCC TCCAGGATTC
    CCAGGCAACA GGGGACTGCC AGGACCACCT GGTGCACAAG GAGCAAGGGG TGATCCAGGC
    ATTCCTGGGC TTCAAGGGCA TCCTGGGACC CCAGGATTTC CAGGTGCTAA AGGTTTTAGA
    GGTCCAGAAG GTGATACAGG AGCGCCAGGC TGTCCAGGCA CCCCTGGTCC ACCATGTGAC
    ACACAATTAC CAGGGCCACC TGGACTCAGA GGTGTCATAG GCCTGCCAGG TTCTCAAGGC
    TTACCAGGCT TTAAAGGTCA GAAAGGAGAC AGGGGCCTAG CTGGGCCACC TGGAATCAAG
    GGTCTCAAGG GCTCTCCTGG CTCACAGGGC CCCCCAGGTC CTCCAGGGTC CCGAGGACTT
    CCAGGACTAC CAGGCAATAA AGGACAACCT GGCTTTCCAG GTCAAACAGG AAGCAAAGGG
    ATTCAAGGAC CCCAAGGATT CCCTGGACTC CCAGGAACAC AAGGCCAAAT GGGAATTCCT
    GGTGTAAAAG GGGAAGAAGG AAACATGGGT CCACCTGGGC CTAGTGGAGA ATGTGGGGAT
    ATGGGAATGA AAGGTGAAAG AGGGCTTCCA GGAGGTGGTG GCAGCATTAA CATTCGGCTT
    GAGAAAGGAC AAAAAGGAGA ACCAGGGTTT CCTGGTGAGA ATGGATTTAG AGGTGAAAGA
    GGTGAAAAAG GAAATACAGG TTTCAGAGGT GCCCCAGGAT TTCCAGGTAA CAACGGTGCC
    CCAGGACTTC CAGGTGACCA TGGTGACATT GGACTGATGG GATTTCCAGG ATCACGTGGT
    TTCCCAGGAC CAAAGGGCTC TAAGGGAGTT ACAGGTTTCA AAGGACTACC AGGTGACCAG
    GGTGATGTTG GCTCACCAGG AATGCCTGGA AACCCAGGAC TGACAGGCCC CAAAGGACCT
    AAAGGAAAGA GAGGTGACCC AACTCTTCTG CTTGGTACAC AAGGTCAGAA AGGGCCTCCT
    GGAGATCTAG GATTACCAGG ATTCTGTGGA TTCCCAGGTG AGAAAGGTTC ACCAGGTATC
    CGAGGGCAAC CTGGAAGACC AGGATCCAAG GGCGAGCCTG GATACCCAGG AATACCTGGA
    TTTCCAGGAG CTACAGGTCC TCCAGGATTG CCAGGAGAAC CTGGAGAAAA AGGGAAACAT
    GGAATTTTGG GACCTCCAGG GCTGCTAGGA TTAACTGGAT CACCAGGTAG AAAAGGTCTT
    CCTGGACTGC CTGGACTGGA TGGCTTGGAC GGACTAAAAG GTCAAAAAGG TTCTGCAGGA
    GCTCCAGGTC AAAGTGAAAC AGGGCGCCCA GGATACTCTG GAGAACCTGG ACCAAAAGGA
    GAGAGAGGTG AACCTGGATG GCCTGGTATT TCCATTCCAG GCACCCCTGG AGAAAGAGGA
    TTCCCAGGAT TCCCAGGTAG AAGAGGACCT GTTGGACCCA TTGGACCTAG GGGACCATCT
    CCCAGTAGTG CTCCTCCTGG TCTTCCAGGT GATCAAGGAC CACCTGGTTT AGATGGCATC
    AGAGGTCAGC CTGGGAATCC TGGTCCTCCT GGAGAGACTG TCTTTGTACG AGGAGATCCA
    GGTGACACCG GTATTCGAGG GGCACCAGGC ATTCCTGGAC ACCGAGGGCT ACAAGGAGCC
    AGAGGTCTGC CAGGAAATCA AGGAAGAGAA GGTCCAAAGG GTCCAATGGG AATCCATGGC
    TTACAGGGTC CACCTGGTGC TGTAGGACAA CCAGGAGACC AAGGTTTTCA AGGAAAACCT
    GGTCCTAAAG GTCCCACAGG GTTCATAGGT GAGCCTGGTG AACCAGGAAA AACAGATGAC
    TCATGTCCTG CAATCCCAGG GCCTCCAGGG GAAGCAGGAC ATAGAGGAGA TGATGGTTCT
    ATAGGATTAC CAGGGCCAAT AGGTCACCCT GGACCCCAAG GAAGGAAAGG TGAAGAAGGG
    TCATGTGGCC TGCCAGGTCA AGATGGCTTA CCAGGAGCAC CTGGACCACC AGGTGACCAA
    GGGCATGTGG GAGAACAAGG ATATGCTGGA CCACAAGGTC CACCTGGCCA GACTGGTATC
    CCAGGACCAC CAGGGCCCCA TGTTAGATCT TCAAGTGGAT TCTTGCTCGT CCTTCATAGT
    CAGTCAGACA GAGAACCACT TTGTCCACAG GGGATGCCAA AATTATGGAC TGGCTATAGT
    CTGCTGTACC TGGAAGGACA AGAGAAAGCT CATAATCAAG ATCTTGGTCT GGCAGGATCT
    TGTCTTCCTG TATTTAATAC CATGCCCTTT GCTTACTGTA ACATCAACCA AGTTTGTTAC
    TATGCCAGCC GAAATGATAA GTCCTACTGG TTGTCAAGTG CCGCTCCCTT ACCAATGACA
    CCACTGTCTG AGGAAGAAAT ACAACCATAT ATAAGCAGAT GTGCTGTATG TGAGGCCCCA
    GCCCAAGCAG TAGCAGTTCA CAGCCAAGAT CAGTCAATTC CTCCCTGCCC AATGAACTGG
    AGAAGTCTTT GGATAGGATA TTCTTTTCTG ATGCACACTG GATCTGGTGA TCAGGGAGGT
    GGGCAGTCCC TTATGTCACC AGGAAGCTGC TTAGAAGACT TCAGATCAGC ACCGTTCATT
    GAATGCCAGG GTCAGCGGGG AACTTGTCAG TTCTTTGCTA ATGAATACAG CTTTTGGCTG
    ACAACAGTGA TGCCTGACTT GCAGTTTGCA TCAGCCCCCT TGTCAGGAAC TCTTAAAGAA
    GGACAAGAGC AGAGGAAAAA AATCAGTAGA TGCCAGGTAT GCCTGAAGCA TGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39728
    Clone ID Related Accession (Same CDS sequence) XM_015276986.1 , XM_015276987.1 , XM_015276987.2 , XM_015276986.2
    Accession Version XM_015276987.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5037bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product collagen alpha-4(IV) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471743.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGCGTTAA CAAAGGATTC ATTTGAACGA ATTAAGTGGC TTATCAGAAC TGCCTGGTGG 
    CTGCTGATTG TTTTTTCAGC ACAAGAAATT GATGGGGGAG GATACGCATA TATTGACCCA
    TGTGGGGGAC AGGATTGTTC GGTTTGTCGG TGCTTTCCAG AAAAAGGATC AAGAGGTCAA
    CCTGGTGAGC TGGGAGCTCA AGGTCCAATT GGATCGTTAG GACCTACAGG ACCAGCTGGT
    CTGCCAGGGG AAAAAGGACA GAGAGGTGAG AATGGGCAGC CTGGGCCAGC TGGAGAAAAA
    GGTGATAAGG GCCCAACTGG AGTCCCAGGA TTTCCTGGTT TAGATGGTGT TCCTGGATTG
    CCAGGAAGTG AAGGACCCAG GGGCAAGCAC GGTGTAGATG GCTGCAATGG CTCAAGAGGA
    GATCCAGGAT TTCCAGGCGA AAATGGATAT ATTGGACCAA GAGGTCCTTA CGGAAACCCA
    GGGCAAAAAG GAGAAAAAGG AAATTCAGTG TATGTTTCGT ATTATGGAAA AGGACCCCCA
    GGAGACAGAG GTGATCCTGG CCCACCTGGT ATGCCCGGAC CTAGAGGATC GGCAGGTACA
    ACAGGCCCAC CTGGATACCC AGGCCAACCA GGCATGCCCG GTATTCCAGG TTACCCTGGT
    TTGCCAGGTG AACAGGGAAA TCCAGGTATT GGAGTGGATG GACAAAAGGG GGAGCCGGGT
    GATGTTGGAC TTCCCGGGCC ACCTGGGTCA CCACTGTTAG TTGGACCTCC TGGAGCTCAG
    CTGTTCAAAG GAGAAAAGGG CCAAAAAGGA CTACCTGGGA TAACGGGATA CAGAGGACCA
    CATGGACCTA AAGGAATACC TGGGAAAGGA GAAAAGGGTG AAAAAGGCAT TCCTGGATTC
    CCTGGATTGC GGGGTAGCCC TGGTTCTCAT GGACCTGCAG GCTTTCCTGG AATAAAGGGA
    GAAATGGGTG TTGCTGGTTT TCCTGGGAAA CCTGGCTATC CAGGCATCAA GGGGGATCCT
    GGAGAAAAAG GGCTCCCCGG TCCTCCTGGA ACTCCACTGC TTCCTATGAA AGGCCCACCA
    GGAGATCCTG GATTTCCAGG TCCTGCGGGT GACATGGGGT CAGTTGGACC TTCTGGCCCT
    GTGGGTATGC TAGGAAGACC AGGAGACGAT GGAACAAGTC TGCCTGGCCT GCCAGGAATA
    AGTGGGGCAC CAGGACCTCA AGGTTTTCAG GGTGACCCTG GTTTGCCTGG AACAGGTGAA
    GCAATTCCAG GAAGACCTGG ATTTCCTGGA CCACCTGGAC TACCAGGACA GCCAGGAAGA
    CAAGGCTTAC CTGGACTACC TAGTGTAATT TGTACTGAGA GAGGGATTCC TGGGGAACCT
    GGAGCAAAAG GTCAGATAGG CCTTCCGGGT AGAAAAGGTG AAAAAGGAGA AAAAGGAAAT
    CAAGGCCTCT GCTCATGTAG TGCTGGTCCT CCTGGTCCCC GTGGTATACA AGGACCTTCA
    GGTACTCAAG GGAAGAAAGG ACAAATGGGT TACCCTGGAA GACGTGGAGA AAAGGGTGAT
    TCAGGCTTAT CTGGTGCAGT GGGATCACCA GGACTCCCTG GGACACCTGG TTCTGGAGGA
    CAACATGGTG AAAAAGGAGA GAAGGGTGAC CAGGGAAGAG TTTCAATAAA AGGAATAAAA
    GGTGAAAGAG GTCCTACTGG GGTTCCAGGA TTTCCAGGCC AAAAAGGTAA TGATGGCAGA
    GATGGGGATT TAGGATTGCC TGGGGAAAAA GGAGATGAGG GTGATTCTGG AGTACCAATA
    CCAGGTGATA AAGGATTTCC TGGAGTTCCA GGACTCCCTG GAGTAAAAGG ACCAATGGGA
    TTGCCTGGTT TGGGGTTTCC TGGCCCTCCG GGAGTTAGAG GTACCCCTGG AGACTTTGGT
    GATGATGGTA ACATTGGTCT GCCTGGTCCA AAGGGTCAGA AAGGTGACAC TATCTGTATT
    ACATCACCGT ACCCTGGAAA CCCAGGTCCC CCAGGTTTTA AAGGTGTTCA AGGACCAAAG
    GGTTTAAAAG GACTCCCTGG TCTTCCTGGA CGAAGTGGCT TTGATGGGCC AAAAGGCCAG
    CAAGGCAGGC CAGGAGCAGG AATCCCTGGA CCAGAAGGCT TCCGAGGCAC AGCTGGTGAT
    CCTGGTGATG AAGGTGAAAG AGGATCTACC ATTGATGGTA AAAATGGTGC TCCAGGTCCA
    CCTGGCATAG ATGGCCAGAA GGGTGTTTCA GGTGATACTA CATATGGCCC TCCAGGATTC
    CCAGGCAACA GGGGACTGCC AGGACCACCT GGTGCACAAG GAGCAAGGGG TGATCCAGGC
    ATTCCTGGGC TTCAAGGGCA TCCTGGGACC CCAGGATTTC CAGGTGCTAA AGGTTTTAGA
    GGTCCAGAAG GTGATACAGG AGCGCCAGGC TGTCCAGGCA CCCCTGGTCC ACCATGTGAC
    ACACAATTAC CAGGGCCACC TGGACTCAGA GGTGTCATAG GCCTGCCAGG TTCTCAAGGC
    TTACCAGGCT TTAAAGGTCA GAAAGGAGAC AGGGGCCTAG CTGGGCCACC TGGAATCAAG
    GGTCTCAAGG GCTCTCCTGG CTCACAGGGC CCCCCAGGTC CTCCAGGGTC CCGAGGACTT
    CCAGGACTAC CAGGCAATAA AGGACAACCT GGCTTTCCAG GTCAAACAGG AAGCAAAGGG
    ATTCAAGGAC CCCAAGGATT CCCTGGACTC CCAGGAACAC AAGGCCAAAT GGGAATTCCT
    GGTGTAAAAG GGGAAGAAGG AAACATGGGT CCACCTGGGC CTAGTGGAGA ATGTGGGGAT
    ATGGGAATGA AAGGTGAAAG AGGGCTTCCA GGAGGTGGTG GCAGCATTAA CATTCGGCTT
    GAGAAAGGAC AAAAAGGAGA ACCAGGGTTT CCTGGTGAGA ATGGATTTAG AGGTGAAAGA
    GGTGAAAAAG GAAATACAGG TTTCAGAGGT GCCCCAGGAT TTCCAGGTAA CAACGGTGCC
    CCAGGACTTC CAGGTGACCA TGGTGACATT GGACTGATGG GATTTCCAGG ATCACGTGGT
    TTCCCAGGAC CAAAGGGCTC TAAGGGAGTT ACAGGTTTCA AAGGACTACC AGGTGACCAG
    GGTGATGTTG GCTCACCAGG AATGCCTGGA AACCCAGGAC TGACAGGCCC CAAAGGACCT
    AAAGGAAAGA GAGGTGACCC AACTCTTCTG CTTGGTACAC AAGGTCAGAA AGGGCCTCCT
    GGAGATCTAG GATTACCAGG ATTCTGTGGA TTCCCAGGTG AGAAAGGTTC ACCAGGTATC
    CGAGGGCAAC CTGGAAGACC AGGATCCAAG GGCGAGCCTG GATACCCAGG AATACCTGGA
    TTTCCAGGAG CTACAGGTCC TCCAGGATTG CCAGGAGAAC CTGGAGAAAA AGGGAAACAT
    GGAATTTTGG GACCTCCAGG GCTGCTAGGA TTAACTGGAT CACCAGGTAG AAAAGGTCTT
    CCTGGACTGC CTGGACTGGA TGGCTTGGAC GGACTAAAAG GTCAAAAAGG TTCTGCAGGA
    GCTCCAGGTC AAAGTGAAAC AGGGCGCCCA GGATACTCTG GAGAACCTGG ACCAAAAGGA
    GAGAGAGGTG AACCTGGATG GCCTGGTATT TCCATTCCAG GCACCCCTGG AGAAAGAGGA
    TTCCCAGGAT TCCCAGGTAG AAGAGGACCT GTTGGACCCA TTGGACCTAG GGGACCATCT
    CCCAGTAGTG CTCCTCCTGG TCTTCCAGGT GATCAAGGAC CACCTGGTTT AGATGGCATC
    AGAGGTCAGC CTGGGAATCC TGGTCCTCCT GGAGAGACTG TCTTTGTACG AGGAGATCCA
    GGTGACACCG GTATTCGAGG GGCACCAGGC ATTCCTGGAC ACCGAGGGCT ACAAGGAGCC
    AGAGGTCTGC CAGGAAATCA AGGAAGAGAA GGTCCAAAGG GTCCAATGGG AATCCATGGC
    TTACAGGGTC CACCTGGTGC TGTAGGACAA CCAGGAGACC AAGGTTTTCA AGGAAAACCT
    GGTCCTAAAG GTCCCACAGG GTTCATAGGT GAGCCTGGTG AACCAGGAAA AACAGATGAC
    TCATGTCCTG CAATCCCAGG GCCTCCAGGG GAAGCAGGAC ATAGAGGAGA TGATGGTTCT
    ATAGGATTAC CAGGGCCAAT AGGTCACCCT GGACCCCAAG GAAGGAAAGG TGAAGAAGGG
    TCATGTGGCC TGCCAGGTCA AGATGGCTTA CCAGGAGCAC CTGGACCACC AGGTGACCAA
    GGGCATGTGG GAGAACAAGG ATATGCTGGA CCACAAGGTC CACCTGGCCA GACTGGTATC
    CCAGGACCAC CAGGGCCCCA TGTTAGATCT TCAAGTGGAT TCTTGCTCGT CCTTCATAGT
    CAGTCAGACA GAGAACCACT TTGTCCACAG GGGATGCCAA AATTATGGAC TGGCTATAGT
    CTGCTGTACC TGGAAGGACA AGAGAAAGCT CATAATCAAG ATCTTGGTCT GGCAGGATCT
    TGTCTTCCTG TATTTAATAC CATGCCCTTT GCTTACTGTA ACATCAACCA AGTTTGTTAC
    TATGCCAGCC GAAATGATAA GTCCTACTGG TTGTCAAGTG CCGCTCCCTT ACCAATGACA
    CCACTGTCTG AGGAAGAAAT ACAACCATAT ATAAGCAGAT GTGCTGTATG TGAGGCCCCA
    GCCCAAGCAG TAGCAGTTCA CAGCCAAGAT CAGTCAATTC CTCCCTGCCC AATGAACTGG
    AGAAGTCTTT GGATAGGATA TTCTTTTCTG ATGCACACTG GATCTGGTGA TCAGGGAGGT
    GGGCAGTCCC TTATGTCACC AGGAAGCTGC TTAGAAGACT TCAGATCAGC ACCGTTCATT
    GAATGCCAGG GTCAGCGGGG AACTTGTCAG TTCTTTGCTA ATGAATACAG CTTTTGGCTG
    ACAACAGTGA TGCCTGACTT GCAGTTTGCA TCAGCCCCCT TGTCAGGAAC TCTTAAAGAA
    GGACAAGAGC AGAGGAAAAA AATCAGTAGA TGCCAGGTAT GCCTGAAGCA TGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015132473.1
    CDS298..5334
    Translation

    Target ORF information:

    RefSeq Version XM_015276987.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus collagen, type IV, alpha 4 (COL4A4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015276987.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGCGTTAA CAAAGGATTC ATTTGAACGA ATTAAGTGGC TTATCAGAAC TGCCTGGTGG 
    CTGCTGATTG TTTTTTCAGC ACAAGAAATT GATGGGGGAG GATACGCATA TATTGACCCA
    TGTGGGGGAC AGGATTGTTC GGTTTGTCGG TGCTTTCCAG AAAAAGGATC AAGAGGTCAA
    CCTGGTGAGC TGGGAGCTCA AGGTCCAATT GGATCGTTAG GACCTACAGG ACCAGCTGGT
    CTGCCAGGGG AAAAAGGACA GAGAGGTGAG AATGGGCAGC CTGGGCCAGC TGGAGAAAAA
    GGTGATAAGG GCCCAACTGG AGTCCCAGGA TTTCCTGGTT TAGATGGTGT TCCTGGATTG
    CCAGGAAGTG AAGGACCCAG GGGCAAGCAC GGTGTAGATG GCTGCAATGG CTCAAGAGGA
    GATCCAGGAT TTCCAGGCGA AAATGGATAT ATTGGACCAA GAGGTCCTTA CGGAAACCCA
    GGGCAAAAAG GAGAAAAAGG AAATTCAGTG TATGTTTCGT ATTATGGAAA AGGACCCCCA
    GGAGACAGAG GTGATCCTGG CCCACCTGGT ATGCCCGGAC CTAGAGGATC GGCAGGTACA
    ACAGGCCCAC CTGGATACCC AGGCCAACCA GGCATGCCCG GTATTCCAGG TTACCCTGGT
    TTGCCAGGTG AACAGGGAAA TCCAGGTATT GGAGTGGATG GACAAAAGGG GGAGCCGGGT
    GATGTTGGAC TTCCCGGGCC ACCTGGGTCA CCACTGTTAG TTGGACCTCC TGGAGCTCAG
    CTGTTCAAAG GAGAAAAGGG CCAAAAAGGA CTACCTGGGA TAACGGGATA CAGAGGACCA
    CATGGACCTA AAGGAATACC TGGGAAAGGA GAAAAGGGTG AAAAAGGCAT TCCTGGATTC
    CCTGGATTGC GGGGTAGCCC TGGTTCTCAT GGACCTGCAG GCTTTCCTGG AATAAAGGGA
    GAAATGGGTG TTGCTGGTTT TCCTGGGAAA CCTGGCTATC CAGGCATCAA GGGGGATCCT
    GGAGAAAAAG GGCTCCCCGG TCCTCCTGGA ACTCCACTGC TTCCTATGAA AGGCCCACCA
    GGAGATCCTG GATTTCCAGG TCCTGCGGGT GACATGGGGT CAGTTGGACC TTCTGGCCCT
    GTGGGTATGC TAGGAAGACC AGGAGACGAT GGAACAAGTC TGCCTGGCCT GCCAGGAATA
    AGTGGGGCAC CAGGACCTCA AGGTTTTCAG GGTGACCCTG GTTTGCCTGG AACAGGTGAA
    GCAATTCCAG GAAGACCTGG ATTTCCTGGA CCACCTGGAC TACCAGGACA GCCAGGAAGA
    CAAGGCTTAC CTGGACTACC TAGTGTAATT TGTACTGAGA GAGGGATTCC TGGGGAACCT
    GGAGCAAAAG GTCAGATAGG CCTTCCGGGT AGAAAAGGTG AAAAAGGAGA AAAAGGAAAT
    CAAGGCCTCT GCTCATGTAG TGCTGGTCCT CCTGGTCCCC GTGGTATACA AGGACCTTCA
    GGTACTCAAG GGAAGAAAGG ACAAATGGGT TACCCTGGAA GACGTGGAGA AAAGGGTGAT
    TCAGGCTTAT CTGGTGCAGT GGGATCACCA GGACTCCCTG GGACACCTGG TTCTGGAGGA
    CAACATGGTG AAAAAGGAGA GAAGGGTGAC CAGGGAAGAG TTTCAATAAA AGGAATAAAA
    GGTGAAAGAG GTCCTACTGG GGTTCCAGGA TTTCCAGGCC AAAAAGGTAA TGATGGCAGA
    GATGGGGATT TAGGATTGCC TGGGGAAAAA GGAGATGAGG GTGATTCTGG AGTACCAATA
    CCAGGTGATA AAGGATTTCC TGGAGTTCCA GGACTCCCTG GAGTAAAAGG ACCAATGGGA
    TTGCCTGGTT TGGGGTTTCC TGGCCCTCCG GGAGTTAGAG GTACCCCTGG AGACTTTGGT
    GATGATGGTA ACATTGGTCT GCCTGGTCCA AAGGGTCAGA AAGGTGACAC TATCTGTATT
    ACATCACCGT ACCCTGGAAA CCCAGGTCCC CCAGGTTTTA AAGGTGTTCA AGGACCAAAG
    GGTTTAAAAG GACTCCCTGG TCTTCCTGGA CGAAGTGGCT TTGATGGGCC AAAAGGCCAG
    CAAGGCAGGC CAGGAGCAGG AATCCCTGGA CCAGAAGGCT TCCGAGGCAC AGCTGGTGAT
    CCTGGTGATG AAGGTGAAAG AGGATCTACC ATTGATGGTA AAAATGGTGC TCCAGGTCCA
    CCTGGCATAG ATGGCCAGAA GGGTGTTTCA GGTGATACTA CATATGGCCC TCCAGGATTC
    CCAGGCAACA GGGGACTGCC AGGACCACCT GGTGCACAAG GAGCAAGGGG TGATCCAGGC
    ATTCCTGGGC TTCAAGGGCA TCCTGGGACC CCAGGATTTC CAGGTGCTAA AGGTTTTAGA
    GGTCCAGAAG GTGATACAGG AGCGCCAGGC TGTCCAGGCA CCCCTGGTCC ACCATGTGAC
    ACACAATTAC CAGGGCCACC TGGACTCAGA GGTGTCATAG GCCTGCCAGG TTCTCAAGGC
    TTACCAGGCT TTAAAGGTCA GAAAGGAGAC AGGGGCCTAG CTGGGCCACC TGGAATCAAG
    GGTCTCAAGG GCTCTCCTGG CTCACAGGGC CCCCCAGGTC CTCCAGGGTC CCGAGGACTT
    CCAGGACTAC CAGGCAATAA AGGACAACCT GGCTTTCCAG GTCAAACAGG AAGCAAAGGG
    ATTCAAGGAC CCCAAGGATT CCCTGGACTC CCAGGAACAC AAGGCCAAAT GGGAATTCCT
    GGTGTAAAAG GGGAAGAAGG AAACATGGGT CCACCTGGGC CTAGTGGAGA ATGTGGGGAT
    ATGGGAATGA AAGGTGAAAG AGGGCTTCCA GGAGGTGGTG GCAGCATTAA CATTCGGCTT
    GAGAAAGGAC AAAAAGGAGA ACCAGGGTTT CCTGGTGAGA ATGGATTTAG AGGTGAAAGA
    GGTGAAAAAG GAAATACAGG TTTCAGAGGT GCCCCAGGAT TTCCAGGTAA CAACGGTGCC
    CCAGGACTTC CAGGTGACCA TGGTGACATT GGACTGATGG GATTTCCAGG ATCACGTGGT
    TTCCCAGGAC CAAAGGGCTC TAAGGGAGTT ACAGGTTTCA AAGGACTACC AGGTGACCAG
    GGTGATGTTG GCTCACCAGG AATGCCTGGA AACCCAGGAC TGACAGGCCC CAAAGGACCT
    AAAGGAAAGA GAGGTGACCC AACTCTTCTG CTTGGTACAC AAGGTCAGAA AGGGCCTCCT
    GGAGATCTAG GATTACCAGG ATTCTGTGGA TTCCCAGGTG AGAAAGGTTC ACCAGGTATC
    CGAGGGCAAC CTGGAAGACC AGGATCCAAG GGCGAGCCTG GATACCCAGG AATACCTGGA
    TTTCCAGGAG CTACAGGTCC TCCAGGATTG CCAGGAGAAC CTGGAGAAAA AGGGAAACAT
    GGAATTTTGG GACCTCCAGG GCTGCTAGGA TTAACTGGAT CACCAGGTAG AAAAGGTCTT
    CCTGGACTGC CTGGACTGGA TGGCTTGGAC GGACTAAAAG GTCAAAAAGG TTCTGCAGGA
    GCTCCAGGTC AAAGTGAAAC AGGGCGCCCA GGATACTCTG GAGAACCTGG ACCAAAAGGA
    GAGAGAGGTG AACCTGGATG GCCTGGTATT TCCATTCCAG GCACCCCTGG AGAAAGAGGA
    TTCCCAGGAT TCCCAGGTAG AAGAGGACCT GTTGGACCCA TTGGACCTAG GGGACCATCT
    CCCAGTAGTG CTCCTCCTGG TCTTCCAGGT GATCAAGGAC CACCTGGTTT AGATGGCATC
    AGAGGTCAGC CTGGGAATCC TGGTCCTCCT GGAGAGACTG TCTTTGTACG AGGAGATCCA
    GGTGACACCG GTATTCGAGG GGCACCAGGC ATTCCTGGAC ACCGAGGGCT ACAAGGAGCC
    AGAGGTCTGC CAGGAAATCA AGGAAGAGAA GGTCCAAAGG GTCCAATGGG AATCCATGGC
    TTACAGGGTC CACCTGGTGC TGTAGGACAA CCAGGAGACC AAGGTTTTCA AGGAAAACCT
    GGTCCTAAAG GTCCCACAGG GTTCATAGGT GAGCCTGGTG AACCAGGAAA AACAGATGAC
    TCATGTCCTG CAATCCCAGG GCCTCCAGGG GAAGCAGGAC ATAGAGGAGA TGATGGTTCT
    ATAGGATTAC CAGGGCCAAT AGGTCACCCT GGACCCCAAG GAAGGAAAGG TGAAGAAGGG
    TCATGTGGCC TGCCAGGTCA AGATGGCTTA CCAGGAGCAC CTGGACCACC AGGTGACCAA
    GGGCATGTGG GAGAACAAGG ATATGCTGGA CCACAAGGTC CACCTGGCCA GACTGGTATC
    CCAGGACCAC CAGGGCCCCA TGTTAGATCT TCAAGTGGAT TCTTGCTCGT CCTTCATAGT
    CAGTCAGACA GAGAACCACT TTGTCCACAG GGGATGCCAA AATTATGGAC TGGCTATAGT
    CTGCTGTACC TGGAAGGACA AGAGAAAGCT CATAATCAAG ATCTTGGTCT GGCAGGATCT
    TGTCTTCCTG TATTTAATAC CATGCCCTTT GCTTACTGTA ACATCAACCA AGTTTGTTAC
    TATGCCAGCC GAAATGATAA GTCCTACTGG TTGTCAAGTG CCGCTCCCTT ACCAATGACA
    CCACTGTCTG AGGAAGAAAT ACAACCATAT ATAAGCAGAT GTGCTGTATG TGAGGCCCCA
    GCCCAAGCAG TAGCAGTTCA CAGCCAAGAT CAGTCAATTC CTCCCTGCCC AATGAACTGG
    AGAAGTCTTT GGATAGGATA TTCTTTTCTG ATGCACACTG GATCTGGTGA TCAGGGAGGT
    GGGCAGTCCC TTATGTCACC AGGAAGCTGC TTAGAAGACT TCAGATCAGC ACCGTTCATT
    GAATGCCAGG GTCAGCGGGG AACTTGTCAG TTCTTTGCTA ATGAATACAG CTTTTGGCTG
    ACAACAGTGA TGCCTGACTT GCAGTTTGCA TCAGCCCCCT TGTCAGGAAC TCTTAAAGAA
    GGACAAGAGC AGAGGAAAAA AATCAGTAGA TGCCAGGTAT GCCTGAAGCA TGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39728
    Clone ID Related Accession (Same CDS sequence) XM_015276986.1 , XM_015276987.1 , XM_015276987.2 , XM_015276986.2
    Accession Version XM_015276987.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5037bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product collagen alpha-4(IV) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015276987.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGCGTTAA CAAAGGATTC ATTTGAACGA ATTAAGTGGC TTATCAGAAC TGCCTGGTGG 
    CTGCTGATTG TTTTTTCAGC ACAAGAAATT GATGGGGGAG GATACGCATA TATTGACCCA
    TGTGGGGGAC AGGATTGTTC GGTTTGTCGG TGCTTTCCAG AAAAAGGATC AAGAGGTCAA
    CCTGGTGAGC TGGGAGCTCA AGGTCCAATT GGATCGTTAG GACCTACAGG ACCAGCTGGT
    CTGCCAGGGG AAAAAGGACA GAGAGGTGAG AATGGGCAGC CTGGGCCAGC TGGAGAAAAA
    GGTGATAAGG GCCCAACTGG AGTCCCAGGA TTTCCTGGTT TAGATGGTGT TCCTGGATTG
    CCAGGAAGTG AAGGACCCAG GGGCAAGCAC GGTGTAGATG GCTGCAATGG CTCAAGAGGA
    GATCCAGGAT TTCCAGGCGA AAATGGATAT ATTGGACCAA GAGGTCCTTA CGGAAACCCA
    GGGCAAAAAG GAGAAAAAGG AAATTCAGTG TATGTTTCGT ATTATGGAAA AGGACCCCCA
    GGAGACAGAG GTGATCCTGG CCCACCTGGT ATGCCCGGAC CTAGAGGATC GGCAGGTACA
    ACAGGCCCAC CTGGATACCC AGGCCAACCA GGCATGCCCG GTATTCCAGG TTACCCTGGT
    TTGCCAGGTG AACAGGGAAA TCCAGGTATT GGAGTGGATG GACAAAAGGG GGAGCCGGGT
    GATGTTGGAC TTCCCGGGCC ACCTGGGTCA CCACTGTTAG TTGGACCTCC TGGAGCTCAG
    CTGTTCAAAG GAGAAAAGGG CCAAAAAGGA CTACCTGGGA TAACGGGATA CAGAGGACCA
    CATGGACCTA AAGGAATACC TGGGAAAGGA GAAAAGGGTG AAAAAGGCAT TCCTGGATTC
    CCTGGATTGC GGGGTAGCCC TGGTTCTCAT GGACCTGCAG GCTTTCCTGG AATAAAGGGA
    GAAATGGGTG TTGCTGGTTT TCCTGGGAAA CCTGGCTATC CAGGCATCAA GGGGGATCCT
    GGAGAAAAAG GGCTCCCCGG TCCTCCTGGA ACTCCACTGC TTCCTATGAA AGGCCCACCA
    GGAGATCCTG GATTTCCAGG TCCTGCGGGT GACATGGGGT CAGTTGGACC TTCTGGCCCT
    GTGGGTATGC TAGGAAGACC AGGAGACGAT GGAACAAGTC TGCCTGGCCT GCCAGGAATA
    AGTGGGGCAC CAGGACCTCA AGGTTTTCAG GGTGACCCTG GTTTGCCTGG AACAGGTGAA
    GCAATTCCAG GAAGACCTGG ATTTCCTGGA CCACCTGGAC TACCAGGACA GCCAGGAAGA
    CAAGGCTTAC CTGGACTACC TAGTGTAATT TGTACTGAGA GAGGGATTCC TGGGGAACCT
    GGAGCAAAAG GTCAGATAGG CCTTCCGGGT AGAAAAGGTG AAAAAGGAGA AAAAGGAAAT
    CAAGGCCTCT GCTCATGTAG TGCTGGTCCT CCTGGTCCCC GTGGTATACA AGGACCTTCA
    GGTACTCAAG GGAAGAAAGG ACAAATGGGT TACCCTGGAA GACGTGGAGA AAAGGGTGAT
    TCAGGCTTAT CTGGTGCAGT GGGATCACCA GGACTCCCTG GGACACCTGG TTCTGGAGGA
    CAACATGGTG AAAAAGGAGA GAAGGGTGAC CAGGGAAGAG TTTCAATAAA AGGAATAAAA
    GGTGAAAGAG GTCCTACTGG GGTTCCAGGA TTTCCAGGCC AAAAAGGTAA TGATGGCAGA
    GATGGGGATT TAGGATTGCC TGGGGAAAAA GGAGATGAGG GTGATTCTGG AGTACCAATA
    CCAGGTGATA AAGGATTTCC TGGAGTTCCA GGACTCCCTG GAGTAAAAGG ACCAATGGGA
    TTGCCTGGTT TGGGGTTTCC TGGCCCTCCG GGAGTTAGAG GTACCCCTGG AGACTTTGGT
    GATGATGGTA ACATTGGTCT GCCTGGTCCA AAGGGTCAGA AAGGTGACAC TATCTGTATT
    ACATCACCGT ACCCTGGAAA CCCAGGTCCC CCAGGTTTTA AAGGTGTTCA AGGACCAAAG
    GGTTTAAAAG GACTCCCTGG TCTTCCTGGA CGAAGTGGCT TTGATGGGCC AAAAGGCCAG
    CAAGGCAGGC CAGGAGCAGG AATCCCTGGA CCAGAAGGCT TCCGAGGCAC AGCTGGTGAT
    CCTGGTGATG AAGGTGAAAG AGGATCTACC ATTGATGGTA AAAATGGTGC TCCAGGTCCA
    CCTGGCATAG ATGGCCAGAA GGGTGTTTCA GGTGATACTA CATATGGCCC TCCAGGATTC
    CCAGGCAACA GGGGACTGCC AGGACCACCT GGTGCACAAG GAGCAAGGGG TGATCCAGGC
    ATTCCTGGGC TTCAAGGGCA TCCTGGGACC CCAGGATTTC CAGGTGCTAA AGGTTTTAGA
    GGTCCAGAAG GTGATACAGG AGCGCCAGGC TGTCCAGGCA CCCCTGGTCC ACCATGTGAC
    ACACAATTAC CAGGGCCACC TGGACTCAGA GGTGTCATAG GCCTGCCAGG TTCTCAAGGC
    TTACCAGGCT TTAAAGGTCA GAAAGGAGAC AGGGGCCTAG CTGGGCCACC TGGAATCAAG
    GGTCTCAAGG GCTCTCCTGG CTCACAGGGC CCCCCAGGTC CTCCAGGGTC CCGAGGACTT
    CCAGGACTAC CAGGCAATAA AGGACAACCT GGCTTTCCAG GTCAAACAGG AAGCAAAGGG
    ATTCAAGGAC CCCAAGGATT CCCTGGACTC CCAGGAACAC AAGGCCAAAT GGGAATTCCT
    GGTGTAAAAG GGGAAGAAGG AAACATGGGT CCACCTGGGC CTAGTGGAGA ATGTGGGGAT
    ATGGGAATGA AAGGTGAAAG AGGGCTTCCA GGAGGTGGTG GCAGCATTAA CATTCGGCTT
    GAGAAAGGAC AAAAAGGAGA ACCAGGGTTT CCTGGTGAGA ATGGATTTAG AGGTGAAAGA
    GGTGAAAAAG GAAATACAGG TTTCAGAGGT GCCCCAGGAT TTCCAGGTAA CAACGGTGCC
    CCAGGACTTC CAGGTGACCA TGGTGACATT GGACTGATGG GATTTCCAGG ATCACGTGGT
    TTCCCAGGAC CAAAGGGCTC TAAGGGAGTT ACAGGTTTCA AAGGACTACC AGGTGACCAG
    GGTGATGTTG GCTCACCAGG AATGCCTGGA AACCCAGGAC TGACAGGCCC CAAAGGACCT
    AAAGGAAAGA GAGGTGACCC AACTCTTCTG CTTGGTACAC AAGGTCAGAA AGGGCCTCCT
    GGAGATCTAG GATTACCAGG ATTCTGTGGA TTCCCAGGTG AGAAAGGTTC ACCAGGTATC
    CGAGGGCAAC CTGGAAGACC AGGATCCAAG GGCGAGCCTG GATACCCAGG AATACCTGGA
    TTTCCAGGAG CTACAGGTCC TCCAGGATTG CCAGGAGAAC CTGGAGAAAA AGGGAAACAT
    GGAATTTTGG GACCTCCAGG GCTGCTAGGA TTAACTGGAT CACCAGGTAG AAAAGGTCTT
    CCTGGACTGC CTGGACTGGA TGGCTTGGAC GGACTAAAAG GTCAAAAAGG TTCTGCAGGA
    GCTCCAGGTC AAAGTGAAAC AGGGCGCCCA GGATACTCTG GAGAACCTGG ACCAAAAGGA
    GAGAGAGGTG AACCTGGATG GCCTGGTATT TCCATTCCAG GCACCCCTGG AGAAAGAGGA
    TTCCCAGGAT TCCCAGGTAG AAGAGGACCT GTTGGACCCA TTGGACCTAG GGGACCATCT
    CCCAGTAGTG CTCCTCCTGG TCTTCCAGGT GATCAAGGAC CACCTGGTTT AGATGGCATC
    AGAGGTCAGC CTGGGAATCC TGGTCCTCCT GGAGAGACTG TCTTTGTACG AGGAGATCCA
    GGTGACACCG GTATTCGAGG GGCACCAGGC ATTCCTGGAC ACCGAGGGCT ACAAGGAGCC
    AGAGGTCTGC CAGGAAATCA AGGAAGAGAA GGTCCAAAGG GTCCAATGGG AATCCATGGC
    TTACAGGGTC CACCTGGTGC TGTAGGACAA CCAGGAGACC AAGGTTTTCA AGGAAAACCT
    GGTCCTAAAG GTCCCACAGG GTTCATAGGT GAGCCTGGTG AACCAGGAAA AACAGATGAC
    TCATGTCCTG CAATCCCAGG GCCTCCAGGG GAAGCAGGAC ATAGAGGAGA TGATGGTTCT
    ATAGGATTAC CAGGGCCAAT AGGTCACCCT GGACCCCAAG GAAGGAAAGG TGAAGAAGGG
    TCATGTGGCC TGCCAGGTCA AGATGGCTTA CCAGGAGCAC CTGGACCACC AGGTGACCAA
    GGGCATGTGG GAGAACAAGG ATATGCTGGA CCACAAGGTC CACCTGGCCA GACTGGTATC
    CCAGGACCAC CAGGGCCCCA TGTTAGATCT TCAAGTGGAT TCTTGCTCGT CCTTCATAGT
    CAGTCAGACA GAGAACCACT TTGTCCACAG GGGATGCCAA AATTATGGAC TGGCTATAGT
    CTGCTGTACC TGGAAGGACA AGAGAAAGCT CATAATCAAG ATCTTGGTCT GGCAGGATCT
    TGTCTTCCTG TATTTAATAC CATGCCCTTT GCTTACTGTA ACATCAACCA AGTTTGTTAC
    TATGCCAGCC GAAATGATAA GTCCTACTGG TTGTCAAGTG CCGCTCCCTT ACCAATGACA
    CCACTGTCTG AGGAAGAAAT ACAACCATAT ATAAGCAGAT GTGCTGTATG TGAGGCCCCA
    GCCCAAGCAG TAGCAGTTCA CAGCCAAGAT CAGTCAATTC CTCCCTGCCC AATGAACTGG
    AGAAGTCTTT GGATAGGATA TTCTTTTCTG ATGCACACTG GATCTGGTGA TCAGGGAGGT
    GGGCAGTCCC TTATGTCACC AGGAAGCTGC TTAGAAGACT TCAGATCAGC ACCGTTCATT
    GAATGCCAGG GTCAGCGGGG AACTTGTCAG TTCTTTGCTA ATGAATACAG CTTTTGGCTG
    ACAACAGTGA TGCCTGACTT GCAGTTTGCA TCAGCCCCCT TGTCAGGAAC TCTTAAAGAA
    GGACAAGAGC AGAGGAAAAA AATCAGTAGA TGCCAGGTAT GCCTGAAGCA TGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015132473.1
    CDS280..5316
    Translation

    Target ORF information:

    RefSeq Version XM_015276987.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus collagen type IV alpha 4 chain (COL4A4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015276987.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGCGTTAA CAAAGGATTC ATTTGAACGA ATTAAGTGGC TTATCAGAAC TGCCTGGTGG 
    CTGCTGATTG TTTTTTCAGC ACAAGAAATT GATGGGGGAG GATACGCATA TATTGACCCA
    TGTGGGGGAC AGGATTGTTC GGTTTGTCGG TGCTTTCCAG AAAAAGGATC AAGAGGTCAA
    CCTGGTGAGC TGGGAGCTCA AGGTCCAATT GGATCGTTAG GACCTACAGG ACCAGCTGGT
    CTGCCAGGGG AAAAAGGACA GAGAGGTGAG AATGGGCAGC CTGGGCCAGC TGGAGAAAAA
    GGTGATAAGG GCCCAACTGG AGTCCCAGGA TTTCCTGGTT TAGATGGTGT TCCTGGATTG
    CCAGGAAGTG AAGGACCCAG GGGCAAGCAC GGTGTAGATG GCTGCAATGG CTCAAGAGGA
    GATCCAGGAT TTCCAGGCGA AAATGGATAT ATTGGACCAA GAGGTCCTTA CGGAAACCCA
    GGGCAAAAAG GAGAAAAAGG AAATTCAGTG TATGTTTCGT ATTATGGAAA AGGACCCCCA
    GGAGACAGAG GTGATCCTGG CCCACCTGGT ATGCCCGGAC CTAGAGGATC GGCAGGTACA
    ACAGGCCCAC CTGGATACCC AGGCCAACCA GGCATGCCCG GTATTCCAGG TTACCCTGGT
    TTGCCAGGTG AACAGGGAAA TCCAGGTATT GGAGTGGATG GACAAAAGGG GGAGCCGGGT
    GATGTTGGAC TTCCCGGGCC ACCTGGGTCA CCACTGTTAG TTGGACCTCC TGGAGCTCAG
    CTGTTCAAAG GAGAAAAGGG CCAAAAAGGA CTACCTGGGA TAACGGGATA CAGAGGACCA
    CATGGACCTA AAGGAATACC TGGGAAAGGA GAAAAGGGTG AAAAAGGCAT TCCTGGATTC
    CCTGGATTGC GGGGTAGCCC TGGTTCTCAT GGACCTGCAG GCTTTCCTGG AATAAAGGGA
    GAAATGGGTG TTGCTGGTTT TCCTGGGAAA CCTGGCTATC CAGGCATCAA GGGGGATCCT
    GGAGAAAAAG GGCTCCCCGG TCCTCCTGGA ACTCCACTGC TTCCTATGAA AGGCCCACCA
    GGAGATCCTG GATTTCCAGG TCCTGCGGGT GACATGGGGT CAGTTGGACC TTCTGGCCCT
    GTGGGTATGC TAGGAAGACC AGGAGACGAT GGAACAAGTC TGCCTGGCCT GCCAGGAATA
    AGTGGGGCAC CAGGACCTCA AGGTTTTCAG GGTGACCCTG GTTTGCCTGG AACAGGTGAA
    GCAATTCCAG GAAGACCTGG ATTTCCTGGA CCACCTGGAC TACCAGGACA GCCAGGAAGA
    CAAGGCTTAC CTGGACTACC TAGTGTAATT TGTACTGAGA GAGGGATTCC TGGGGAACCT
    GGAGCAAAAG GTCAGATAGG CCTTCCGGGT AGAAAAGGTG AAAAAGGAGA AAAAGGAAAT
    CAAGGCCTCT GCTCATGTAG TGCTGGTCCT CCTGGTCCCC GTGGTATACA AGGACCTTCA
    GGTACTCAAG GGAAGAAAGG ACAAATGGGT TACCCTGGAA GACGTGGAGA AAAGGGTGAT
    TCAGGCTTAT CTGGTGCAGT GGGATCACCA GGACTCCCTG GGACACCTGG TTCTGGAGGA
    CAACATGGTG AAAAAGGAGA GAAGGGTGAC CAGGGAAGAG TTTCAATAAA AGGAATAAAA
    GGTGAAAGAG GTCCTACTGG GGTTCCAGGA TTTCCAGGCC AAAAAGGTAA TGATGGCAGA
    GATGGGGATT TAGGATTGCC TGGGGAAAAA GGAGATGAGG GTGATTCTGG AGTACCAATA
    CCAGGTGATA AAGGATTTCC TGGAGTTCCA GGACTCCCTG GAGTAAAAGG ACCAATGGGA
    TTGCCTGGTT TGGGGTTTCC TGGCCCTCCG GGAGTTAGAG GTACCCCTGG AGACTTTGGT
    GATGATGGTA ACATTGGTCT GCCTGGTCCA AAGGGTCAGA AAGGTGACAC TATCTGTATT
    ACATCACCGT ACCCTGGAAA CCCAGGTCCC CCAGGTTTTA AAGGTGTTCA AGGACCAAAG
    GGTTTAAAAG GACTCCCTGG TCTTCCTGGA CGAAGTGGCT TTGATGGGCC AAAAGGCCAG
    CAAGGCAGGC CAGGAGCAGG AATCCCTGGA CCAGAAGGCT TCCGAGGCAC AGCTGGTGAT
    CCTGGTGATG AAGGTGAAAG AGGATCTACC ATTGATGGTA AAAATGGTGC TCCAGGTCCA
    CCTGGCATAG ATGGCCAGAA GGGTGTTTCA GGTGATACTA CATATGGCCC TCCAGGATTC
    CCAGGCAACA GGGGACTGCC AGGACCACCT GGTGCACAAG GAGCAAGGGG TGATCCAGGC
    ATTCCTGGGC TTCAAGGGCA TCCTGGGACC CCAGGATTTC CAGGTGCTAA AGGTTTTAGA
    GGTCCAGAAG GTGATACAGG AGCGCCAGGC TGTCCAGGCA CCCCTGGTCC ACCATGTGAC
    ACACAATTAC CAGGGCCACC TGGACTCAGA GGTGTCATAG GCCTGCCAGG TTCTCAAGGC
    TTACCAGGCT TTAAAGGTCA GAAAGGAGAC AGGGGCCTAG CTGGGCCACC TGGAATCAAG
    GGTCTCAAGG GCTCTCCTGG CTCACAGGGC CCCCCAGGTC CTCCAGGGTC CCGAGGACTT
    CCAGGACTAC CAGGCAATAA AGGACAACCT GGCTTTCCAG GTCAAACAGG AAGCAAAGGG
    ATTCAAGGAC CCCAAGGATT CCCTGGACTC CCAGGAACAC AAGGCCAAAT GGGAATTCCT
    GGTGTAAAAG GGGAAGAAGG AAACATGGGT CCACCTGGGC CTAGTGGAGA ATGTGGGGAT
    ATGGGAATGA AAGGTGAAAG AGGGCTTCCA GGAGGTGGTG GCAGCATTAA CATTCGGCTT
    GAGAAAGGAC AAAAAGGAGA ACCAGGGTTT CCTGGTGAGA ATGGATTTAG AGGTGAAAGA
    GGTGAAAAAG GAAATACAGG TTTCAGAGGT GCCCCAGGAT TTCCAGGTAA CAACGGTGCC
    CCAGGACTTC CAGGTGACCA TGGTGACATT GGACTGATGG GATTTCCAGG ATCACGTGGT
    TTCCCAGGAC CAAAGGGCTC TAAGGGAGTT ACAGGTTTCA AAGGACTACC AGGTGACCAG
    GGTGATGTTG GCTCACCAGG AATGCCTGGA AACCCAGGAC TGACAGGCCC CAAAGGACCT
    AAAGGAAAGA GAGGTGACCC AACTCTTCTG CTTGGTACAC AAGGTCAGAA AGGGCCTCCT
    GGAGATCTAG GATTACCAGG ATTCTGTGGA TTCCCAGGTG AGAAAGGTTC ACCAGGTATC
    CGAGGGCAAC CTGGAAGACC AGGATCCAAG GGCGAGCCTG GATACCCAGG AATACCTGGA
    TTTCCAGGAG CTACAGGTCC TCCAGGATTG CCAGGAGAAC CTGGAGAAAA AGGGAAACAT
    GGAATTTTGG GACCTCCAGG GCTGCTAGGA TTAACTGGAT CACCAGGTAG AAAAGGTCTT
    CCTGGACTGC CTGGACTGGA TGGCTTGGAC GGACTAAAAG GTCAAAAAGG TTCTGCAGGA
    GCTCCAGGTC AAAGTGAAAC AGGGCGCCCA GGATACTCTG GAGAACCTGG ACCAAAAGGA
    GAGAGAGGTG AACCTGGATG GCCTGGTATT TCCATTCCAG GCACCCCTGG AGAAAGAGGA
    TTCCCAGGAT TCCCAGGTAG AAGAGGACCT GTTGGACCCA TTGGACCTAG GGGACCATCT
    CCCAGTAGTG CTCCTCCTGG TCTTCCAGGT GATCAAGGAC CACCTGGTTT AGATGGCATC
    AGAGGTCAGC CTGGGAATCC TGGTCCTCCT GGAGAGACTG TCTTTGTACG AGGAGATCCA
    GGTGACACCG GTATTCGAGG GGCACCAGGC ATTCCTGGAC ACCGAGGGCT ACAAGGAGCC
    AGAGGTCTGC CAGGAAATCA AGGAAGAGAA GGTCCAAAGG GTCCAATGGG AATCCATGGC
    TTACAGGGTC CACCTGGTGC TGTAGGACAA CCAGGAGACC AAGGTTTTCA AGGAAAACCT
    GGTCCTAAAG GTCCCACAGG GTTCATAGGT GAGCCTGGTG AACCAGGAAA AACAGATGAC
    TCATGTCCTG CAATCCCAGG GCCTCCAGGG GAAGCAGGAC ATAGAGGAGA TGATGGTTCT
    ATAGGATTAC CAGGGCCAAT AGGTCACCCT GGACCCCAAG GAAGGAAAGG TGAAGAAGGG
    TCATGTGGCC TGCCAGGTCA AGATGGCTTA CCAGGAGCAC CTGGACCACC AGGTGACCAA
    GGGCATGTGG GAGAACAAGG ATATGCTGGA CCACAAGGTC CACCTGGCCA GACTGGTATC
    CCAGGACCAC CAGGGCCCCA TGTTAGATCT TCAAGTGGAT TCTTGCTCGT CCTTCATAGT
    CAGTCAGACA GAGAACCACT TTGTCCACAG GGGATGCCAA AATTATGGAC TGGCTATAGT
    CTGCTGTACC TGGAAGGACA AGAGAAAGCT CATAATCAAG ATCTTGGTCT GGCAGGATCT
    TGTCTTCCTG TATTTAATAC CATGCCCTTT GCTTACTGTA ACATCAACCA AGTTTGTTAC
    TATGCCAGCC GAAATGATAA GTCCTACTGG TTGTCAAGTG CCGCTCCCTT ACCAATGACA
    CCACTGTCTG AGGAAGAAAT ACAACCATAT ATAAGCAGAT GTGCTGTATG TGAGGCCCCA
    GCCCAAGCAG TAGCAGTTCA CAGCCAAGAT CAGTCAATTC CTCCCTGCCC AATGAACTGG
    AGAAGTCTTT GGATAGGATA TTCTTTTCTG ATGCACACTG GATCTGGTGA TCAGGGAGGT
    GGGCAGTCCC TTATGTCACC AGGAAGCTGC TTAGAAGACT TCAGATCAGC ACCGTTCATT
    GAATGCCAGG GTCAGCGGGG AACTTGTCAG TTCTTTGCTA ATGAATACAG CTTTTGGCTG
    ACAACAGTGA TGCCTGACTT GCAGTTTGCA TCAGCCCCCT TGTCAGGAAC TCTTAAAGAA
    GGACAAGAGC AGAGGAAAAA AATCAGTAGA TGCCAGGTAT GCCTGAAGCA TGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39728
    Clone ID Related Accession (Same CDS sequence) XM_015276986.1 , XM_015276987.1 , XM_015276987.2 , XM_015276986.2
    Accession Version XM_015276986.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5037bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product collagen alpha-4(IV) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015276986.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGCGTTAA CAAAGGATTC ATTTGAACGA ATTAAGTGGC TTATCAGAAC TGCCTGGTGG 
    CTGCTGATTG TTTTTTCAGC ACAAGAAATT GATGGGGGAG GATACGCATA TATTGACCCA
    TGTGGGGGAC AGGATTGTTC GGTTTGTCGG TGCTTTCCAG AAAAAGGATC AAGAGGTCAA
    CCTGGTGAGC TGGGAGCTCA AGGTCCAATT GGATCGTTAG GACCTACAGG ACCAGCTGGT
    CTGCCAGGGG AAAAAGGACA GAGAGGTGAG AATGGGCAGC CTGGGCCAGC TGGAGAAAAA
    GGTGATAAGG GCCCAACTGG AGTCCCAGGA TTTCCTGGTT TAGATGGTGT TCCTGGATTG
    CCAGGAAGTG AAGGACCCAG GGGCAAGCAC GGTGTAGATG GCTGCAATGG CTCAAGAGGA
    GATCCAGGAT TTCCAGGCGA AAATGGATAT ATTGGACCAA GAGGTCCTTA CGGAAACCCA
    GGGCAAAAAG GAGAAAAAGG AAATTCAGTG TATGTTTCGT ATTATGGAAA AGGACCCCCA
    GGAGACAGAG GTGATCCTGG CCCACCTGGT ATGCCCGGAC CTAGAGGATC GGCAGGTACA
    ACAGGCCCAC CTGGATACCC AGGCCAACCA GGCATGCCCG GTATTCCAGG TTACCCTGGT
    TTGCCAGGTG AACAGGGAAA TCCAGGTATT GGAGTGGATG GACAAAAGGG GGAGCCGGGT
    GATGTTGGAC TTCCCGGGCC ACCTGGGTCA CCACTGTTAG TTGGACCTCC TGGAGCTCAG
    CTGTTCAAAG GAGAAAAGGG CCAAAAAGGA CTACCTGGGA TAACGGGATA CAGAGGACCA
    CATGGACCTA AAGGAATACC TGGGAAAGGA GAAAAGGGTG AAAAAGGCAT TCCTGGATTC
    CCTGGATTGC GGGGTAGCCC TGGTTCTCAT GGACCTGCAG GCTTTCCTGG AATAAAGGGA
    GAAATGGGTG TTGCTGGTTT TCCTGGGAAA CCTGGCTATC CAGGCATCAA GGGGGATCCT
    GGAGAAAAAG GGCTCCCCGG TCCTCCTGGA ACTCCACTGC TTCCTATGAA AGGCCCACCA
    GGAGATCCTG GATTTCCAGG TCCTGCGGGT GACATGGGGT CAGTTGGACC TTCTGGCCCT
    GTGGGTATGC TAGGAAGACC AGGAGACGAT GGAACAAGTC TGCCTGGCCT GCCAGGAATA
    AGTGGGGCAC CAGGACCTCA AGGTTTTCAG GGTGACCCTG GTTTGCCTGG AACAGGTGAA
    GCAATTCCAG GAAGACCTGG ATTTCCTGGA CCACCTGGAC TACCAGGACA GCCAGGAAGA
    CAAGGCTTAC CTGGACTACC TAGTGTAATT TGTACTGAGA GAGGGATTCC TGGGGAACCT
    GGAGCAAAAG GTCAGATAGG CCTTCCGGGT AGAAAAGGTG AAAAAGGAGA AAAAGGAAAT
    CAAGGCCTCT GCTCATGTAG TGCTGGTCCT CCTGGTCCCC GTGGTATACA AGGACCTTCA
    GGTACTCAAG GGAAGAAAGG ACAAATGGGT TACCCTGGAA GACGTGGAGA AAAGGGTGAT
    TCAGGCTTAT CTGGTGCAGT GGGATCACCA GGACTCCCTG GGACACCTGG TTCTGGAGGA
    CAACATGGTG AAAAAGGAGA GAAGGGTGAC CAGGGAAGAG TTTCAATAAA AGGAATAAAA
    GGTGAAAGAG GTCCTACTGG GGTTCCAGGA TTTCCAGGCC AAAAAGGTAA TGATGGCAGA
    GATGGGGATT TAGGATTGCC TGGGGAAAAA GGAGATGAGG GTGATTCTGG AGTACCAATA
    CCAGGTGATA AAGGATTTCC TGGAGTTCCA GGACTCCCTG GAGTAAAAGG ACCAATGGGA
    TTGCCTGGTT TGGGGTTTCC TGGCCCTCCG GGAGTTAGAG GTACCCCTGG AGACTTTGGT
    GATGATGGTA ACATTGGTCT GCCTGGTCCA AAGGGTCAGA AAGGTGACAC TATCTGTATT
    ACATCACCGT ACCCTGGAAA CCCAGGTCCC CCAGGTTTTA AAGGTGTTCA AGGACCAAAG
    GGTTTAAAAG GACTCCCTGG TCTTCCTGGA CGAAGTGGCT TTGATGGGCC AAAAGGCCAG
    CAAGGCAGGC CAGGAGCAGG AATCCCTGGA CCAGAAGGCT TCCGAGGCAC AGCTGGTGAT
    CCTGGTGATG AAGGTGAAAG AGGATCTACC ATTGATGGTA AAAATGGTGC TCCAGGTCCA
    CCTGGCATAG ATGGCCAGAA GGGTGTTTCA GGTGATACTA CATATGGCCC TCCAGGATTC
    CCAGGCAACA GGGGACTGCC AGGACCACCT GGTGCACAAG GAGCAAGGGG TGATCCAGGC
    ATTCCTGGGC TTCAAGGGCA TCCTGGGACC CCAGGATTTC CAGGTGCTAA AGGTTTTAGA
    GGTCCAGAAG GTGATACAGG AGCGCCAGGC TGTCCAGGCA CCCCTGGTCC ACCATGTGAC
    ACACAATTAC CAGGGCCACC TGGACTCAGA GGTGTCATAG GCCTGCCAGG TTCTCAAGGC
    TTACCAGGCT TTAAAGGTCA GAAAGGAGAC AGGGGCCTAG CTGGGCCACC TGGAATCAAG
    GGTCTCAAGG GCTCTCCTGG CTCACAGGGC CCCCCAGGTC CTCCAGGGTC CCGAGGACTT
    CCAGGACTAC CAGGCAATAA AGGACAACCT GGCTTTCCAG GTCAAACAGG AAGCAAAGGG
    ATTCAAGGAC CCCAAGGATT CCCTGGACTC CCAGGAACAC AAGGCCAAAT GGGAATTCCT
    GGTGTAAAAG GGGAAGAAGG AAACATGGGT CCACCTGGGC CTAGTGGAGA ATGTGGGGAT
    ATGGGAATGA AAGGTGAAAG AGGGCTTCCA GGAGGTGGTG GCAGCATTAA CATTCGGCTT
    GAGAAAGGAC AAAAAGGAGA ACCAGGGTTT CCTGGTGAGA ATGGATTTAG AGGTGAAAGA
    GGTGAAAAAG GAAATACAGG TTTCAGAGGT GCCCCAGGAT TTCCAGGTAA CAACGGTGCC
    CCAGGACTTC CAGGTGACCA TGGTGACATT GGACTGATGG GATTTCCAGG ATCACGTGGT
    TTCCCAGGAC CAAAGGGCTC TAAGGGAGTT ACAGGTTTCA AAGGACTACC AGGTGACCAG
    GGTGATGTTG GCTCACCAGG AATGCCTGGA AACCCAGGAC TGACAGGCCC CAAAGGACCT
    AAAGGAAAGA GAGGTGACCC AACTCTTCTG CTTGGTACAC AAGGTCAGAA AGGGCCTCCT
    GGAGATCTAG GATTACCAGG ATTCTGTGGA TTCCCAGGTG AGAAAGGTTC ACCAGGTATC
    CGAGGGCAAC CTGGAAGACC AGGATCCAAG GGCGAGCCTG GATACCCAGG AATACCTGGA
    TTTCCAGGAG CTACAGGTCC TCCAGGATTG CCAGGAGAAC CTGGAGAAAA AGGGAAACAT
    GGAATTTTGG GACCTCCAGG GCTGCTAGGA TTAACTGGAT CACCAGGTAG AAAAGGTCTT
    CCTGGACTGC CTGGACTGGA TGGCTTGGAC GGACTAAAAG GTCAAAAAGG TTCTGCAGGA
    GCTCCAGGTC AAAGTGAAAC AGGGCGCCCA GGATACTCTG GAGAACCTGG ACCAAAAGGA
    GAGAGAGGTG AACCTGGATG GCCTGGTATT TCCATTCCAG GCACCCCTGG AGAAAGAGGA
    TTCCCAGGAT TCCCAGGTAG AAGAGGACCT GTTGGACCCA TTGGACCTAG GGGACCATCT
    CCCAGTAGTG CTCCTCCTGG TCTTCCAGGT GATCAAGGAC CACCTGGTTT AGATGGCATC
    AGAGGTCAGC CTGGGAATCC TGGTCCTCCT GGAGAGACTG TCTTTGTACG AGGAGATCCA
    GGTGACACCG GTATTCGAGG GGCACCAGGC ATTCCTGGAC ACCGAGGGCT ACAAGGAGCC
    AGAGGTCTGC CAGGAAATCA AGGAAGAGAA GGTCCAAAGG GTCCAATGGG AATCCATGGC
    TTACAGGGTC CACCTGGTGC TGTAGGACAA CCAGGAGACC AAGGTTTTCA AGGAAAACCT
    GGTCCTAAAG GTCCCACAGG GTTCATAGGT GAGCCTGGTG AACCAGGAAA AACAGATGAC
    TCATGTCCTG CAATCCCAGG GCCTCCAGGG GAAGCAGGAC ATAGAGGAGA TGATGGTTCT
    ATAGGATTAC CAGGGCCAAT AGGTCACCCT GGACCCCAAG GAAGGAAAGG TGAAGAAGGG
    TCATGTGGCC TGCCAGGTCA AGATGGCTTA CCAGGAGCAC CTGGACCACC AGGTGACCAA
    GGGCATGTGG GAGAACAAGG ATATGCTGGA CCACAAGGTC CACCTGGCCA GACTGGTATC
    CCAGGACCAC CAGGGCCCCA TGTTAGATCT TCAAGTGGAT TCTTGCTCGT CCTTCATAGT
    CAGTCAGACA GAGAACCACT TTGTCCACAG GGGATGCCAA AATTATGGAC TGGCTATAGT
    CTGCTGTACC TGGAAGGACA AGAGAAAGCT CATAATCAAG ATCTTGGTCT GGCAGGATCT
    TGTCTTCCTG TATTTAATAC CATGCCCTTT GCTTACTGTA ACATCAACCA AGTTTGTTAC
    TATGCCAGCC GAAATGATAA GTCCTACTGG TTGTCAAGTG CCGCTCCCTT ACCAATGACA
    CCACTGTCTG AGGAAGAAAT ACAACCATAT ATAAGCAGAT GTGCTGTATG TGAGGCCCCA
    GCCCAAGCAG TAGCAGTTCA CAGCCAAGAT CAGTCAATTC CTCCCTGCCC AATGAACTGG
    AGAAGTCTTT GGATAGGATA TTCTTTTCTG ATGCACACTG GATCTGGTGA TCAGGGAGGT
    GGGCAGTCCC TTATGTCACC AGGAAGCTGC TTAGAAGACT TCAGATCAGC ACCGTTCATT
    GAATGCCAGG GTCAGCGGGG AACTTGTCAG TTCTTTGCTA ATGAATACAG CTTTTGGCTG
    ACAACAGTGA TGCCTGACTT GCAGTTTGCA TCAGCCCCCT TGTCAGGAAC TCTTAAAGAA
    GGACAAGAGC AGAGGAAAAA AATCAGTAGA TGCCAGGTAT GCCTGAAGCA TGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015132472.1
    CDS276..5312
    Translation

    Target ORF information:

    RefSeq Version XM_015276986.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus collagen type IV alpha 4 chain (COL4A4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015276986.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGCGTTAA CAAAGGATTC ATTTGAACGA ATTAAGTGGC TTATCAGAAC TGCCTGGTGG 
    CTGCTGATTG TTTTTTCAGC ACAAGAAATT GATGGGGGAG GATACGCATA TATTGACCCA
    TGTGGGGGAC AGGATTGTTC GGTTTGTCGG TGCTTTCCAG AAAAAGGATC AAGAGGTCAA
    CCTGGTGAGC TGGGAGCTCA AGGTCCAATT GGATCGTTAG GACCTACAGG ACCAGCTGGT
    CTGCCAGGGG AAAAAGGACA GAGAGGTGAG AATGGGCAGC CTGGGCCAGC TGGAGAAAAA
    GGTGATAAGG GCCCAACTGG AGTCCCAGGA TTTCCTGGTT TAGATGGTGT TCCTGGATTG
    CCAGGAAGTG AAGGACCCAG GGGCAAGCAC GGTGTAGATG GCTGCAATGG CTCAAGAGGA
    GATCCAGGAT TTCCAGGCGA AAATGGATAT ATTGGACCAA GAGGTCCTTA CGGAAACCCA
    GGGCAAAAAG GAGAAAAAGG AAATTCAGTG TATGTTTCGT ATTATGGAAA AGGACCCCCA
    GGAGACAGAG GTGATCCTGG CCCACCTGGT ATGCCCGGAC CTAGAGGATC GGCAGGTACA
    ACAGGCCCAC CTGGATACCC AGGCCAACCA GGCATGCCCG GTATTCCAGG TTACCCTGGT
    TTGCCAGGTG AACAGGGAAA TCCAGGTATT GGAGTGGATG GACAAAAGGG GGAGCCGGGT
    GATGTTGGAC TTCCCGGGCC ACCTGGGTCA CCACTGTTAG TTGGACCTCC TGGAGCTCAG
    CTGTTCAAAG GAGAAAAGGG CCAAAAAGGA CTACCTGGGA TAACGGGATA CAGAGGACCA
    CATGGACCTA AAGGAATACC TGGGAAAGGA GAAAAGGGTG AAAAAGGCAT TCCTGGATTC
    CCTGGATTGC GGGGTAGCCC TGGTTCTCAT GGACCTGCAG GCTTTCCTGG AATAAAGGGA
    GAAATGGGTG TTGCTGGTTT TCCTGGGAAA CCTGGCTATC CAGGCATCAA GGGGGATCCT
    GGAGAAAAAG GGCTCCCCGG TCCTCCTGGA ACTCCACTGC TTCCTATGAA AGGCCCACCA
    GGAGATCCTG GATTTCCAGG TCCTGCGGGT GACATGGGGT CAGTTGGACC TTCTGGCCCT
    GTGGGTATGC TAGGAAGACC AGGAGACGAT GGAACAAGTC TGCCTGGCCT GCCAGGAATA
    AGTGGGGCAC CAGGACCTCA AGGTTTTCAG GGTGACCCTG GTTTGCCTGG AACAGGTGAA
    GCAATTCCAG GAAGACCTGG ATTTCCTGGA CCACCTGGAC TACCAGGACA GCCAGGAAGA
    CAAGGCTTAC CTGGACTACC TAGTGTAATT TGTACTGAGA GAGGGATTCC TGGGGAACCT
    GGAGCAAAAG GTCAGATAGG CCTTCCGGGT AGAAAAGGTG AAAAAGGAGA AAAAGGAAAT
    CAAGGCCTCT GCTCATGTAG TGCTGGTCCT CCTGGTCCCC GTGGTATACA AGGACCTTCA
    GGTACTCAAG GGAAGAAAGG ACAAATGGGT TACCCTGGAA GACGTGGAGA AAAGGGTGAT
    TCAGGCTTAT CTGGTGCAGT GGGATCACCA GGACTCCCTG GGACACCTGG TTCTGGAGGA
    CAACATGGTG AAAAAGGAGA GAAGGGTGAC CAGGGAAGAG TTTCAATAAA AGGAATAAAA
    GGTGAAAGAG GTCCTACTGG GGTTCCAGGA TTTCCAGGCC AAAAAGGTAA TGATGGCAGA
    GATGGGGATT TAGGATTGCC TGGGGAAAAA GGAGATGAGG GTGATTCTGG AGTACCAATA
    CCAGGTGATA AAGGATTTCC TGGAGTTCCA GGACTCCCTG GAGTAAAAGG ACCAATGGGA
    TTGCCTGGTT TGGGGTTTCC TGGCCCTCCG GGAGTTAGAG GTACCCCTGG AGACTTTGGT
    GATGATGGTA ACATTGGTCT GCCTGGTCCA AAGGGTCAGA AAGGTGACAC TATCTGTATT
    ACATCACCGT ACCCTGGAAA CCCAGGTCCC CCAGGTTTTA AAGGTGTTCA AGGACCAAAG
    GGTTTAAAAG GACTCCCTGG TCTTCCTGGA CGAAGTGGCT TTGATGGGCC AAAAGGCCAG
    CAAGGCAGGC CAGGAGCAGG AATCCCTGGA CCAGAAGGCT TCCGAGGCAC AGCTGGTGAT
    CCTGGTGATG AAGGTGAAAG AGGATCTACC ATTGATGGTA AAAATGGTGC TCCAGGTCCA
    CCTGGCATAG ATGGCCAGAA GGGTGTTTCA GGTGATACTA CATATGGCCC TCCAGGATTC
    CCAGGCAACA GGGGACTGCC AGGACCACCT GGTGCACAAG GAGCAAGGGG TGATCCAGGC
    ATTCCTGGGC TTCAAGGGCA TCCTGGGACC CCAGGATTTC CAGGTGCTAA AGGTTTTAGA
    GGTCCAGAAG GTGATACAGG AGCGCCAGGC TGTCCAGGCA CCCCTGGTCC ACCATGTGAC
    ACACAATTAC CAGGGCCACC TGGACTCAGA GGTGTCATAG GCCTGCCAGG TTCTCAAGGC
    TTACCAGGCT TTAAAGGTCA GAAAGGAGAC AGGGGCCTAG CTGGGCCACC TGGAATCAAG
    GGTCTCAAGG GCTCTCCTGG CTCACAGGGC CCCCCAGGTC CTCCAGGGTC CCGAGGACTT
    CCAGGACTAC CAGGCAATAA AGGACAACCT GGCTTTCCAG GTCAAACAGG AAGCAAAGGG
    ATTCAAGGAC CCCAAGGATT CCCTGGACTC CCAGGAACAC AAGGCCAAAT GGGAATTCCT
    GGTGTAAAAG GGGAAGAAGG AAACATGGGT CCACCTGGGC CTAGTGGAGA ATGTGGGGAT
    ATGGGAATGA AAGGTGAAAG AGGGCTTCCA GGAGGTGGTG GCAGCATTAA CATTCGGCTT
    GAGAAAGGAC AAAAAGGAGA ACCAGGGTTT CCTGGTGAGA ATGGATTTAG AGGTGAAAGA
    GGTGAAAAAG GAAATACAGG TTTCAGAGGT GCCCCAGGAT TTCCAGGTAA CAACGGTGCC
    CCAGGACTTC CAGGTGACCA TGGTGACATT GGACTGATGG GATTTCCAGG ATCACGTGGT
    TTCCCAGGAC CAAAGGGCTC TAAGGGAGTT ACAGGTTTCA AAGGACTACC AGGTGACCAG
    GGTGATGTTG GCTCACCAGG AATGCCTGGA AACCCAGGAC TGACAGGCCC CAAAGGACCT
    AAAGGAAAGA GAGGTGACCC AACTCTTCTG CTTGGTACAC AAGGTCAGAA AGGGCCTCCT
    GGAGATCTAG GATTACCAGG ATTCTGTGGA TTCCCAGGTG AGAAAGGTTC ACCAGGTATC
    CGAGGGCAAC CTGGAAGACC AGGATCCAAG GGCGAGCCTG GATACCCAGG AATACCTGGA
    TTTCCAGGAG CTACAGGTCC TCCAGGATTG CCAGGAGAAC CTGGAGAAAA AGGGAAACAT
    GGAATTTTGG GACCTCCAGG GCTGCTAGGA TTAACTGGAT CACCAGGTAG AAAAGGTCTT
    CCTGGACTGC CTGGACTGGA TGGCTTGGAC GGACTAAAAG GTCAAAAAGG TTCTGCAGGA
    GCTCCAGGTC AAAGTGAAAC AGGGCGCCCA GGATACTCTG GAGAACCTGG ACCAAAAGGA
    GAGAGAGGTG AACCTGGATG GCCTGGTATT TCCATTCCAG GCACCCCTGG AGAAAGAGGA
    TTCCCAGGAT TCCCAGGTAG AAGAGGACCT GTTGGACCCA TTGGACCTAG GGGACCATCT
    CCCAGTAGTG CTCCTCCTGG TCTTCCAGGT GATCAAGGAC CACCTGGTTT AGATGGCATC
    AGAGGTCAGC CTGGGAATCC TGGTCCTCCT GGAGAGACTG TCTTTGTACG AGGAGATCCA
    GGTGACACCG GTATTCGAGG GGCACCAGGC ATTCCTGGAC ACCGAGGGCT ACAAGGAGCC
    AGAGGTCTGC CAGGAAATCA AGGAAGAGAA GGTCCAAAGG GTCCAATGGG AATCCATGGC
    TTACAGGGTC CACCTGGTGC TGTAGGACAA CCAGGAGACC AAGGTTTTCA AGGAAAACCT
    GGTCCTAAAG GTCCCACAGG GTTCATAGGT GAGCCTGGTG AACCAGGAAA AACAGATGAC
    TCATGTCCTG CAATCCCAGG GCCTCCAGGG GAAGCAGGAC ATAGAGGAGA TGATGGTTCT
    ATAGGATTAC CAGGGCCAAT AGGTCACCCT GGACCCCAAG GAAGGAAAGG TGAAGAAGGG
    TCATGTGGCC TGCCAGGTCA AGATGGCTTA CCAGGAGCAC CTGGACCACC AGGTGACCAA
    GGGCATGTGG GAGAACAAGG ATATGCTGGA CCACAAGGTC CACCTGGCCA GACTGGTATC
    CCAGGACCAC CAGGGCCCCA TGTTAGATCT TCAAGTGGAT TCTTGCTCGT CCTTCATAGT
    CAGTCAGACA GAGAACCACT TTGTCCACAG GGGATGCCAA AATTATGGAC TGGCTATAGT
    CTGCTGTACC TGGAAGGACA AGAGAAAGCT CATAATCAAG ATCTTGGTCT GGCAGGATCT
    TGTCTTCCTG TATTTAATAC CATGCCCTTT GCTTACTGTA ACATCAACCA AGTTTGTTAC
    TATGCCAGCC GAAATGATAA GTCCTACTGG TTGTCAAGTG CCGCTCCCTT ACCAATGACA
    CCACTGTCTG AGGAAGAAAT ACAACCATAT ATAAGCAGAT GTGCTGTATG TGAGGCCCCA
    GCCCAAGCAG TAGCAGTTCA CAGCCAAGAT CAGTCAATTC CTCCCTGCCC AATGAACTGG
    AGAAGTCTTT GGATAGGATA TTCTTTTCTG ATGCACACTG GATCTGGTGA TCAGGGAGGT
    GGGCAGTCCC TTATGTCACC AGGAAGCTGC TTAGAAGACT TCAGATCAGC ACCGTTCATT
    GAATGCCAGG GTCAGCGGGG AACTTGTCAG TTCTTTGCTA ATGAATACAG CTTTTGGCTG
    ACAACAGTGA TGCCTGACTT GCAGTTTGCA TCAGCCCCCT TGTCAGGAAC TCTTAAAGAA
    GGACAAGAGC AGAGGAAAAA AATCAGTAGA TGCCAGGTAT GCCTGAAGCA TGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39729
    Clone ID Related Accession (Same CDS sequence) XM_015276988.1
    Accession Version XM_015276988.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4959bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product collagen alpha-4(IV) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471743.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTTAA CAAAGGATTC ATTTGAACGA ATTAAGTGGC TTATCAGAAC TGCCTGGTGG 
    CTGCTGATTG TTTTTTCAGC ACAAGAAATT GATGGGGGAG GATACGCATA TATTGACCCA
    TGTGGGGGAC AGGATTGTTC GGTTTGTCGG TGCTTTCCAG AAAAAGGATC AAGAGGTCAA
    CCTGGTGAGC TGGGAGCTCA AGGTCCAATT GGATCGTTAG GACCTACAGG ACCAGCTGGT
    CTGCCAGGGG AAAAAGGACA GAGAGGTGAG AATGGGCAGC CTGGGCCAGC TGGAGAAAAA
    GGTGATAAGG GCCCAACTGG AGTCCCAGGA TTTCCTGGTT TAGATGGTGT TCCTGGATTG
    CCAGGAAGTG AAGGACCCAG GGGCAAGCAC GGTGTAGATG GCTGCAATGG CTCAAGAGGA
    GATCCAGGAT TTCCAGGCGA AAATGGATAT ATTGGACCAA GAGGTCCTTA CGGAAACCCA
    GGGCAAAAAG GAGAAAAAGG AAATTCAGTG TATGTTTCGT ATTATGGAAA AGGACCCCCA
    GGAGACAGAG GTGATCCTGG CCCACCTGGT ATGCCCGGAC CTAGAGGATC GGCAGGTACA
    ACAGGCCCAC CTGGATACCC AGGCCAACCA GGCATGCCCG GTATTCCAGG TTACCCTGGT
    TTGCCAGGTG AACAGGGAAA TCCAGGTATT GGAGTGGATG GACAAAAGGG GGAGCCGGGT
    GATGTTGGAC TTCCCGGGCC ACCTGGGTCA CCACTGTTAG TTGGACCTCC TGGAGCTCAG
    CTGTTCAAAG GAGAAAAGGG CCAAAAAGGA CTACCTGGGA TAACGGGATA CAGAGGACCA
    CATGGACCTA AAGGAATACC TGGGAAAGGA GAAAAGGGTG AAAAAGGCAT TCCTGGATTC
    CCTGGATTGC GGGGTAGCCC TGGTTCTCAT GGACCTGCAG GCTTTCCTGG AATAAAGGGA
    GAAATGGGTG TTGCTGGTTT TCCTGGGAAA CCTGGCTATC CAGGCATCAA GGGGGATCCT
    GGAGAAAAAG GGCTCCCCGG TCCTCCTGGA ACTCCACTGC TTCCTATGAA AGGCCCACCA
    GGAGATCCTG GATTTCCAGG TCCTGCGGGT GACATGGGGT CAGTTGGACC TTCTGGCCCT
    GTGGGTATGC TAGGAAGACC AGGAGACGAT GGAACAAGTC TGCCTGGCCT GCCAGGAATA
    AGTGGGGCAC CAGGACCTCA AGGTTTTCAG GGTGACCCTG GTTTGCCTGG AACAGGTGAA
    GCAATTCCAG GAAGACCTGG ATTTCCTGGA CCACCTGGAC TACCAGGACA GCCAGGAAGA
    CAAGGCTTAC CTGGACTACC TAGTGTAATT TGTACTGAGA GAGGGATTCC TGGGGAACCT
    GGAGCAAAAG GTCAGATAGG CCTTCCGGGT AGAAAAGGTG AAAAAGGAGA AAAAGGAAAT
    CAAGGCCTCT GCTCATGTAG TGCTGGTCCT CCTGGTCCCC GTGGTATACA AGGACCTTCA
    GGTACTCAAG GGAAGAAAGG ACAAATGGGT TACCCTGGAA GACGTGGAGA AAAGGGTGAT
    TCAGGCTTAT CTGGTGCAGT GGGATCACCA GGACTCCCTG GGACACCTGG TTCTGGAGGA
    CAACATGGTG AAAAAGGAGA GAAGGGTGAC CAGGGAAGAG TTTCAATAAA AGGAATAAAA
    GGTGAAAGAG GTCCTACTGG GGTTCCAGGA TTTCCAGGCC AAAAAGGTAA TGATGGCAGA
    GATGGGGATT TAGGATTGCC TGGGGAAAAA GGAGATGAGG GTGATTCTGG AGTACCAATA
    CCAGGTGATA AAGGATTTCC TGGAGTTCCA GGACTCCCTG GAGTAAAAGG ACCAATGGGA
    TTGCCTGGTT TGGGGTTTCC TGGCCCTCCG GGAGTTAGAG GTACCCCTGG AGACTTTGGT
    GATGATGGTA ACATTGGTCT GCCTGGTCCA AAGGGTCAGA AAGGTGACAC TATCTGTATT
    ACATCACCGT ACCCTGGAAA CCCAGGTCCC CCAGGTTTTA AAGGTGTTCA AGGACCAAAG
    GGTTTAAAAG GACTCCCTGG TCTTCCTGGA CGAAGTGGCT TTGATGGGCC AAAAGGCCAG
    CAAGGCAGGC CAGGAGCAGG AATCCCTGGA CCAGAAGGCT TCCGAGGCAC AGCTGGTGAT
    CCTGGTGATG AAGGTGAAAG AGGATCTACC ATTGATGGAT TCCCAGGCAA CAGGGGACTG
    CCAGGACCAC CTGGTGCACA AGGAGCAAGG GGTGATCCAG GCATTCCTGG GCTTCAAGGG
    CATCCTGGGA CCCCAGGATT TCCAGGTGCT AAAGGTTTTA GAGGTCCAGA AGGTGATACA
    GGAGCGCCAG GCTGTCCAGG CACCCCTGGT CCACCATGTG ACACACAATT ACCAGGGCCA
    CCTGGACTCA GAGGTGTCAT AGGCCTGCCA GGTTCTCAAG GCTTACCAGG CTTTAAAGGT
    CAGAAAGGAG ACAGGGGCCT AGCTGGGCCA CCTGGAATCA AGGGTCTCAA GGGCTCTCCT
    GGCTCACAGG GCCCCCCAGG TCCTCCAGGG TCCCGAGGAC TTCCAGGACT ACCAGGCAAT
    AAAGGACAAC CTGGCTTTCC AGGTCAAACA GGAAGCAAAG GGATTCAAGG ACCCCAAGGA
    TTCCCTGGAC TCCCAGGAAC ACAAGGCCAA ATGGGAATTC CTGGTGTAAA AGGGGAAGAA
    GGAAACATGG GTCCACCTGG GCCTAGTGGA GAATGTGGGG ATATGGGAAT GAAAGGTGAA
    AGAGGGCTTC CAGGAGGTGG TGGCAGCATT AACATTCGGC TTGAGAAAGG ACAAAAAGGA
    GAACCAGGGT TTCCTGGTGA GAATGGATTT AGAGGTGAAA GAGGTGAAAA AGGAAATACA
    GGTTTCAGAG GTGCCCCAGG ATTTCCAGGT AACAACGGTG CCCCAGGACT TCCAGGTGAC
    CATGGTGACA TTGGACTGAT GGGATTTCCA GGATCACGTG GTTTCCCAGG ACCAAAGGGC
    TCTAAGGGAG TTACAGGTTT CAAAGGACTA CCAGGTGACC AGGGTGATGT TGGCTCACCA
    GGAATGCCTG GAAACCCAGG ACTGACAGGC CCCAAAGGAC CTAAAGGAAA GAGAGGTGAC
    CCAACTCTTC TGCTTGGTAC ACAAGGTCAG AAAGGGCCTC CTGGAGATCT AGGATTACCA
    GGATTCTGTG GATTCCCAGG TGAGAAAGGT TCACCAGGTA TCCGAGGGCA ACCTGGAAGA
    CCAGGATCCA AGGGCGAGCC TGGATACCCA GGAATACCTG GATTTCCAGG AGCTACAGGT
    CCTCCAGGAT TGCCAGGAGA ACCTGGAGAA AAAGGGAAAC ATGGAATTTT GGGACCTCCA
    GGGCTGCTAG GATTAACTGG ATCACCAGGT AGAAAAGGTC TTCCTGGACT GCCTGGACTG
    GATGGCTTGG ACGGACTAAA AGGTCAAAAA GGTTCTGCAG GAGCTCCAGG TCAAAGTGAA
    ACAGGGCGCC CAGGATACTC TGGAGAACCT GGACCAAAAG GAGAGAGAGG TGAACCTGGA
    TGGCCTGGTA TTTCCATTCC AGGCACCCCT GGAGAAAGAG GATTCCCAGG ATTCCCAGGT
    AGAAGAGGAC CTGTTGGACC CATTGGACCT AGGGGACCAT CTCCCAGTAG TGCTCCTCCT
    GGTCTTCCAG GTGATCAAGG ACCACCTGGT TTAGATGGCA TCAGAGGTCA GCCTGGGAAT
    CCTGGTCCTC CTGGAGAGAC TGTCTTTGTA CGAGGAGATC CAGGTGACAC CGGTATTCGA
    GGGGCACCAG GCATTCCTGG ACACCGAGGG CTACAAGGAG CCAGAGGTCT GCCAGGAAAT
    CAAGGAAGAG AAGGTCCAAA GGGTCCAATG GGAATCCATG GCTTACAGGG TCCACCTGGT
    GCTGTAGGAC AACCAGGAGA CCAAGGTTTT CAAGGAAAAC CTGGTCCTAA AGGTCCCACA
    GGGTTCATAG GTGAGCCTGG TGAACCAGGA AAAACAGATG ACTCATGTCC TGCAATCCCA
    GGGCCTCCAG GGGAAGCAGG ACATAGAGGA GATGATGGTT CTATAGGATT ACCAGGGCCA
    ATAGGTCACC CTGGACCCCA AGGAAGGAAA GGTGAAGAAG GGTCATGTGG CCTGCCAGGT
    CAAGATGGCT TACCAGGAGC ACCTGGACCA CCAGGTGACC AAGGGCATGT GGGAGAACAA
    GGATATGCTG GACCACAAGG TCCACCTGGC CAGACTGGTA TCCCAGGACC ACCAGGGCCC
    CATGTTAGAT CTTCAAGTGG ATTCTTGCTC GTCCTTCATA GTCAGTCAGA CAGAGAACCA
    CTTTGTCCAC AGGGGATGCC AAAATTATGG ACTGGCTATA GTCTGCTGTA CCTGGAAGGA
    CAAGAGAAAG CTCATAATCA AGATCTTGGT CTGGCAGGAT CTTGTCTTCC TGTATTTAAT
    ACCATGCCCT TTGCTTACTG TAACATCAAC CAAGTTTGTT ACTATGCCAG CCGAAATGAT
    AAGTCCTACT GGTTGTCAAG TGCCGCTCCC TTACCAATGA CACCACTGTC TGAGGAAGAA
    ATACAACCAT ATATAAGCAG ATGTGCTGTA TGTGAGGCCC CAGCCCAAGC AGTAGCAGTT
    CACAGCCAAG ATCAGTCAAT TCCTCCCTGC CCAATGAACT GGAGAAGTCT TTGGATAGGA
    TATTCTTTTC TGATGCACAC TGGATCTGGT GATCAGGGAG GTGGGCAGTC CCTTATGTCA
    CCAGGAAGCT GCTTAGAAGA CTTCAGATCA GCACCGTTCA TTGAATGCCA GGGTCAGCGG
    GGAACTTGTC AGTTCTTTGC TAATGAATAC AGCTTTTGGC TGACAACAGT GATGCCTGAC
    TTGCAGTTTG CATCAGCCCC CTTGTCAGGA ACTCTTAAAG AAGGACAAGA GCAGAGGAAA
    AAAATCAGTA GATGCCAGGT ATGCCTGAAG CATGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015132474.1
    CDS237..5195
    Translation

    Target ORF information:

    RefSeq Version XM_015276988.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus collagen, type IV, alpha 4 (COL4A4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015276988.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTTAA CAAAGGATTC ATTTGAACGA ATTAAGTGGC TTATCAGAAC TGCCTGGTGG 
    CTGCTGATTG TTTTTTCAGC ACAAGAAATT GATGGGGGAG GATACGCATA TATTGACCCA
    TGTGGGGGAC AGGATTGTTC GGTTTGTCGG TGCTTTCCAG AAAAAGGATC AAGAGGTCAA
    CCTGGTGAGC TGGGAGCTCA AGGTCCAATT GGATCGTTAG GACCTACAGG ACCAGCTGGT
    CTGCCAGGGG AAAAAGGACA GAGAGGTGAG AATGGGCAGC CTGGGCCAGC TGGAGAAAAA
    GGTGATAAGG GCCCAACTGG AGTCCCAGGA TTTCCTGGTT TAGATGGTGT TCCTGGATTG
    CCAGGAAGTG AAGGACCCAG GGGCAAGCAC GGTGTAGATG GCTGCAATGG CTCAAGAGGA
    GATCCAGGAT TTCCAGGCGA AAATGGATAT ATTGGACCAA GAGGTCCTTA CGGAAACCCA
    GGGCAAAAAG GAGAAAAAGG AAATTCAGTG TATGTTTCGT ATTATGGAAA AGGACCCCCA
    GGAGACAGAG GTGATCCTGG CCCACCTGGT ATGCCCGGAC CTAGAGGATC GGCAGGTACA
    ACAGGCCCAC CTGGATACCC AGGCCAACCA GGCATGCCCG GTATTCCAGG TTACCCTGGT
    TTGCCAGGTG AACAGGGAAA TCCAGGTATT GGAGTGGATG GACAAAAGGG GGAGCCGGGT
    GATGTTGGAC TTCCCGGGCC ACCTGGGTCA CCACTGTTAG TTGGACCTCC TGGAGCTCAG
    CTGTTCAAAG GAGAAAAGGG CCAAAAAGGA CTACCTGGGA TAACGGGATA CAGAGGACCA
    CATGGACCTA AAGGAATACC TGGGAAAGGA GAAAAGGGTG AAAAAGGCAT TCCTGGATTC
    CCTGGATTGC GGGGTAGCCC TGGTTCTCAT GGACCTGCAG GCTTTCCTGG AATAAAGGGA
    GAAATGGGTG TTGCTGGTTT TCCTGGGAAA CCTGGCTATC CAGGCATCAA GGGGGATCCT
    GGAGAAAAAG GGCTCCCCGG TCCTCCTGGA ACTCCACTGC TTCCTATGAA AGGCCCACCA
    GGAGATCCTG GATTTCCAGG TCCTGCGGGT GACATGGGGT CAGTTGGACC TTCTGGCCCT
    GTGGGTATGC TAGGAAGACC AGGAGACGAT GGAACAAGTC TGCCTGGCCT GCCAGGAATA
    AGTGGGGCAC CAGGACCTCA AGGTTTTCAG GGTGACCCTG GTTTGCCTGG AACAGGTGAA
    GCAATTCCAG GAAGACCTGG ATTTCCTGGA CCACCTGGAC TACCAGGACA GCCAGGAAGA
    CAAGGCTTAC CTGGACTACC TAGTGTAATT TGTACTGAGA GAGGGATTCC TGGGGAACCT
    GGAGCAAAAG GTCAGATAGG CCTTCCGGGT AGAAAAGGTG AAAAAGGAGA AAAAGGAAAT
    CAAGGCCTCT GCTCATGTAG TGCTGGTCCT CCTGGTCCCC GTGGTATACA AGGACCTTCA
    GGTACTCAAG GGAAGAAAGG ACAAATGGGT TACCCTGGAA GACGTGGAGA AAAGGGTGAT
    TCAGGCTTAT CTGGTGCAGT GGGATCACCA GGACTCCCTG GGACACCTGG TTCTGGAGGA
    CAACATGGTG AAAAAGGAGA GAAGGGTGAC CAGGGAAGAG TTTCAATAAA AGGAATAAAA
    GGTGAAAGAG GTCCTACTGG GGTTCCAGGA TTTCCAGGCC AAAAAGGTAA TGATGGCAGA
    GATGGGGATT TAGGATTGCC TGGGGAAAAA GGAGATGAGG GTGATTCTGG AGTACCAATA
    CCAGGTGATA AAGGATTTCC TGGAGTTCCA GGACTCCCTG GAGTAAAAGG ACCAATGGGA
    TTGCCTGGTT TGGGGTTTCC TGGCCCTCCG GGAGTTAGAG GTACCCCTGG AGACTTTGGT
    GATGATGGTA ACATTGGTCT GCCTGGTCCA AAGGGTCAGA AAGGTGACAC TATCTGTATT
    ACATCACCGT ACCCTGGAAA CCCAGGTCCC CCAGGTTTTA AAGGTGTTCA AGGACCAAAG
    GGTTTAAAAG GACTCCCTGG TCTTCCTGGA CGAAGTGGCT TTGATGGGCC AAAAGGCCAG
    CAAGGCAGGC CAGGAGCAGG AATCCCTGGA CCAGAAGGCT TCCGAGGCAC AGCTGGTGAT
    CCTGGTGATG AAGGTGAAAG AGGATCTACC ATTGATGGAT TCCCAGGCAA CAGGGGACTG
    CCAGGACCAC CTGGTGCACA AGGAGCAAGG GGTGATCCAG GCATTCCTGG GCTTCAAGGG
    CATCCTGGGA CCCCAGGATT TCCAGGTGCT AAAGGTTTTA GAGGTCCAGA AGGTGATACA
    GGAGCGCCAG GCTGTCCAGG CACCCCTGGT CCACCATGTG ACACACAATT ACCAGGGCCA
    CCTGGACTCA GAGGTGTCAT AGGCCTGCCA GGTTCTCAAG GCTTACCAGG CTTTAAAGGT
    CAGAAAGGAG ACAGGGGCCT AGCTGGGCCA CCTGGAATCA AGGGTCTCAA GGGCTCTCCT
    GGCTCACAGG GCCCCCCAGG TCCTCCAGGG TCCCGAGGAC TTCCAGGACT ACCAGGCAAT
    AAAGGACAAC CTGGCTTTCC AGGTCAAACA GGAAGCAAAG GGATTCAAGG ACCCCAAGGA
    TTCCCTGGAC TCCCAGGAAC ACAAGGCCAA ATGGGAATTC CTGGTGTAAA AGGGGAAGAA
    GGAAACATGG GTCCACCTGG GCCTAGTGGA GAATGTGGGG ATATGGGAAT GAAAGGTGAA
    AGAGGGCTTC CAGGAGGTGG TGGCAGCATT AACATTCGGC TTGAGAAAGG ACAAAAAGGA
    GAACCAGGGT TTCCTGGTGA GAATGGATTT AGAGGTGAAA GAGGTGAAAA AGGAAATACA
    GGTTTCAGAG GTGCCCCAGG ATTTCCAGGT AACAACGGTG CCCCAGGACT TCCAGGTGAC
    CATGGTGACA TTGGACTGAT GGGATTTCCA GGATCACGTG GTTTCCCAGG ACCAAAGGGC
    TCTAAGGGAG TTACAGGTTT CAAAGGACTA CCAGGTGACC AGGGTGATGT TGGCTCACCA
    GGAATGCCTG GAAACCCAGG ACTGACAGGC CCCAAAGGAC CTAAAGGAAA GAGAGGTGAC
    CCAACTCTTC TGCTTGGTAC ACAAGGTCAG AAAGGGCCTC CTGGAGATCT AGGATTACCA
    GGATTCTGTG GATTCCCAGG TGAGAAAGGT TCACCAGGTA TCCGAGGGCA ACCTGGAAGA
    CCAGGATCCA AGGGCGAGCC TGGATACCCA GGAATACCTG GATTTCCAGG AGCTACAGGT
    CCTCCAGGAT TGCCAGGAGA ACCTGGAGAA AAAGGGAAAC ATGGAATTTT GGGACCTCCA
    GGGCTGCTAG GATTAACTGG ATCACCAGGT AGAAAAGGTC TTCCTGGACT GCCTGGACTG
    GATGGCTTGG ACGGACTAAA AGGTCAAAAA GGTTCTGCAG GAGCTCCAGG TCAAAGTGAA
    ACAGGGCGCC CAGGATACTC TGGAGAACCT GGACCAAAAG GAGAGAGAGG TGAACCTGGA
    TGGCCTGGTA TTTCCATTCC AGGCACCCCT GGAGAAAGAG GATTCCCAGG ATTCCCAGGT
    AGAAGAGGAC CTGTTGGACC CATTGGACCT AGGGGACCAT CTCCCAGTAG TGCTCCTCCT
    GGTCTTCCAG GTGATCAAGG ACCACCTGGT TTAGATGGCA TCAGAGGTCA GCCTGGGAAT
    CCTGGTCCTC CTGGAGAGAC TGTCTTTGTA CGAGGAGATC CAGGTGACAC CGGTATTCGA
    GGGGCACCAG GCATTCCTGG ACACCGAGGG CTACAAGGAG CCAGAGGTCT GCCAGGAAAT
    CAAGGAAGAG AAGGTCCAAA GGGTCCAATG GGAATCCATG GCTTACAGGG TCCACCTGGT
    GCTGTAGGAC AACCAGGAGA CCAAGGTTTT CAAGGAAAAC CTGGTCCTAA AGGTCCCACA
    GGGTTCATAG GTGAGCCTGG TGAACCAGGA AAAACAGATG ACTCATGTCC TGCAATCCCA
    GGGCCTCCAG GGGAAGCAGG ACATAGAGGA GATGATGGTT CTATAGGATT ACCAGGGCCA
    ATAGGTCACC CTGGACCCCA AGGAAGGAAA GGTGAAGAAG GGTCATGTGG CCTGCCAGGT
    CAAGATGGCT TACCAGGAGC ACCTGGACCA CCAGGTGACC AAGGGCATGT GGGAGAACAA
    GGATATGCTG GACCACAAGG TCCACCTGGC CAGACTGGTA TCCCAGGACC ACCAGGGCCC
    CATGTTAGAT CTTCAAGTGG ATTCTTGCTC GTCCTTCATA GTCAGTCAGA CAGAGAACCA
    CTTTGTCCAC AGGGGATGCC AAAATTATGG ACTGGCTATA GTCTGCTGTA CCTGGAAGGA
    CAAGAGAAAG CTCATAATCA AGATCTTGGT CTGGCAGGAT CTTGTCTTCC TGTATTTAAT
    ACCATGCCCT TTGCTTACTG TAACATCAAC CAAGTTTGTT ACTATGCCAG CCGAAATGAT
    AAGTCCTACT GGTTGTCAAG TGCCGCTCCC TTACCAATGA CACCACTGTC TGAGGAAGAA
    ATACAACCAT ATATAAGCAG ATGTGCTGTA TGTGAGGCCC CAGCCCAAGC AGTAGCAGTT
    CACAGCCAAG ATCAGTCAAT TCCTCCCTGC CCAATGAACT GGAGAAGTCT TTGGATAGGA
    TATTCTTTTC TGATGCACAC TGGATCTGGT GATCAGGGAG GTGGGCAGTC CCTTATGTCA
    CCAGGAAGCT GCTTAGAAGA CTTCAGATCA GCACCGTTCA TTGAATGCCA GGGTCAGCGG
    GGAACTTGTC AGTTCTTTGC TAATGAATAC AGCTTTTGGC TGACAACAGT GATGCCTGAC
    TTGCAGTTTG CATCAGCCCC CTTGTCAGGA ACTCTTAAAG AAGGACAAGA GCAGAGGAAA
    AAAATCAGTA GATGCCAGGT ATGCCTGAAG CATGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.