COL4A4 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL4A4
Entrez Gene ID	424799
Full Name	collagen type IV alpha 4 chain
General protein information	Preferred Names collagen type IV alpha 4 chain Names collagen alpha-4(IV) chain collagen, type IV, alpha 4
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015276988.1	XP_015132474.1	collagen alpha-4(IV) chain isoform X2
XM_015276986.1	XP_015132472.1	collagen alpha-4(IV) chain isoform X1
XM_015276987.1	XP_015132473.1	collagen alpha-4(IV) chain isoform X1
XM_015276987.2	XP_015132473.1	collagen alpha-4(IV) chain isoform X1
XM_025153677.1	XP_025009445.1	collagen alpha-4(IV) chain isoform X2
XM_015276986.2	XP_015132472.1	collagen alpha-4(IV) chain isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	COL4A4	XP_422615.4
Danio rerio (zebrafish)	col4a4	XP_693954.6

Gene Ontology

The following COL4A4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL4A4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa152836	XM_025153677.1 Latest version!	Gallus gallus collagen type IV alpha 4 chain (COL4A4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa39728	XM_015276987.2 Latest version!	Gallus gallus collagen type IV alpha 4 chain (COL4A4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OGa39728	XM_015276986.2 Latest version!	Gallus gallus collagen type IV alpha 4 chain (COL4A4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OGa39729	XM_015276988.1 Latest version!	Gallus gallus collagen, type IV, alpha 4 (COL4A4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
View Old Accession Versions >>
OGa39728	XM_015276987.1	Gallus gallus collagen, type IV, alpha 4 (COL4A4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OGa39728	XM_015276986.1	Gallus gallus collagen, type IV, alpha 4 (COL4A4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL4A4 ( XM_025153677.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025009445.1 Gallus gallus collagen type IV alpha 4 chain (COL4A4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa152836
Clone ID Related Accession (Same CDS sequence)	XM_025153677.1
Accession Version	XM_025153677.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	collagen alpha-4(IV) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTTAA CAAAGGATTC ATTTGAACGA ATTAAGTGGC TTATCAGAAC TGCCTGGTGG 
CTGCTGATTG TTTTTTCAGC ACAAGAAATT GATGGGGGAG GATACGCATA TATTGACCCA 
TGTGGGGGAC AGGATTGTTC GGTTTGTCGG TGCTTTCCAG AAAAAGGATC AAGAGGTCAA 
CCTGGTGAGC TGGGAGCTCA AGGTCCAATT GGATCGTTAG GACCTACAGG ACCAGCTGGT 
CTGCCAGGGG AAAAAGGACA GAGAGGTGAG AATGGGCAGC CTGGGCCAGC TGGAGAAAAA 
GGTGATAAGG GCCCAACTGG AGTCCCAGGA TTTCCTGGTT TAGATGGTGT TCCTGGATTG 
CCAGGAAGTG AAGGACCCAG GGGCAAGCAC GGTGTAGATG GCTGCAATGG CTCAAGAGGA 
GATCCAGGAT TTCCAGGCGA AAATGGATAT ATTGGACCAA GAGGTCCTTA CGGAAACCCA 
GGGCAAAAAG GAGAAAAAGG AAATTCAGTG TATGTTTCGT ATTATGGAAA AGGACCCCCA 
GGAGACAGAG GTGATCCTGG CCCACCTGGT ATGCCCGGAC CTAGAGGATC GGCAGGTACA 
ACAGGCCCAC CTGGATACCC AGGCCAACCA GGCATGCCCG GTATTCCAGG TTACCCTGGT 
TTGCCAGGTG AACAGGGAAA TCCAGGTATT GGAGTGGATG GACAAAAGGG GGAGCCGGGT 
GATGTTGGAC TTCCCGGGCC ACCTGGGTCA CCACTGTTAG TTGGACCTCC TGGAGCTCAG 
CTGTTCAAAG GAGAAAAGGG CCAAAAAGGA CTACCTGGGA TAACGGGATA CAGAGGACCA 
CATGGACCTA AAGGAATACC TGGGAAAGGA GAAAAGGGTG AAAAAGGCAT TCCTGGATTC 
CCTGGATTGC GGGGTAGCCC TGGTTCTCAT GGACCTGCAG GCTTTCCTGG AATAAAGGGA 
GAAATGGGTG TTGCTGGTTT TCCTGGGAAA CCTGGCTATC CAGGCATCAA GGGGGATCCT 
GGAGAAAAAG GGCTCCCCGG TCCTCCTGGA ACTCCACTGC TTCCTATGAA AGGCCCACCA 
GGAGATCCTG GATTTCCAGG TCCTGCGGGT GACATGGGGT CAGTTGGACC TTCTGGCCCT 
GTGGGTATGC TAGGAAGACC AGGAGACGAT GGAACAAGTC TGCCTGGCCT GCCAGGAATA 
AGTGGGGCAC CAGGACCTCA AGGTTTTCAG GGTGACCCTG GTTTGCCTGG AACAGGTGAA 
GCAATTCCAG GAAGACCTGG ATTTCCTGGA CCACCTGGAC TACCAGGACA GCCAGGAAGA 
CAAGGCTTAC CTGGACTACC TAGTGTAATT TGTACTGAGA GAGGGATTCC TGGGGAACCT 
GGAGCAAAAG GTCAGATAGG CCTTCCGGGT AGAAAAGGTG AAAAAGGAGA AAAAGGAAAT 
CAAGGCCTCT GCTCATGTAG TGCTGGTCCT CCTGGTCCCC GTGGTATACA AGGACCTTCA 
GGTACTCAAG GGAAGAAAGG ACAAATGGGT TACCCTGGAA GACGTGGAGA AAAGGGTGAT 
TCAGGCTTAT CTGGTGCAGT GGGATCACCA GGACTCCCTG GGACACCTGG TTCTGGAGGA 
CAACATGGTG AAAAAGGAGA GAAGGGTGAC CAGGGAAGAG TTTCAATAAA AGGAATAAAA 
GGTGAAAGAG GTCCTACTGG GGTTCCAGGA TTTCCAGGCC AAAAAGGTAA TGATGGCAGA 
GATGGGGATT TAGGATTGCC TGGGGAAAAA GGAGATGAGG GTGATTCTGG AGTACCAATA 
CCAGGTGATA AAGGATTTCC TGGAGTTCCA GGACTCCCTG GAGTAAAAGG ACCAATGGGA 
TTGCCTGGTT TGGGGTTTCC TGGCCCTCCG GGAGTTAGAG GTACCCCTGG AGACTTTGGT 
GATGATGGTA ACATTGGTCT GCCTGGTCCA AAGGGTCAGA AAGGTGACAC TATCTGTATT 
ACATCACCGT ACCCTGGAAA CCCAGGTCCC CCAGGTTTTA AAGGTGTTCA AGGACCAAAG 
GGTTTAAAAG GACTCCCTGG TCTTCCTGGA CGAAGTGGCT TTGATGGGCC AAAAGGCCAG 
CAAGGCAGGC CAGGAGCAGG AATCCCTGGA CCAGAAGGCT TCCGAGGCAC AGCTGGTGAT 
CCTGGTGATG AAGGTGAAAG AGGATCTACC ATTGATGGTA AAAATGGTGC TCCAGGTCCA 
CCTGGCATAG ATGGCCAGAA GGGTGTTTCA GGTGATACTA CATATGGCCC TCCAGGATTC 
CCAGGCAACA GGGGACTGCC AGGACCACCT GGTGCACAAG GAGCAAGGGG TGATCCAGGC 
ATTCCTGGGC TTCAAGGGCA TCCTGGGACC CCAGGATTTC CAGGTGCTAA AGGTTTTAGA 
GGTCCAGAAG GTGATACAGG AGCGCCAGGC TGTCCAGGCA CCCCTGGTCC ACCATGTGAC 
ACACAATTAC CAGGGCCACC TGGACTCAGA GGTGTCATAG GCCTGCCAGG TTCTCAAGGC 
TTACCAGGCT TTAAAGGTCA GAAAGGAGAC AGGGGCCTAG CTGGGCCACC TGGAATCAAG 
GGTCTCAAGG GCTCTCCTGG CTCACAGGGC CCCCCAGGTC CTCCAGGGTC CCGAGGACTT 
CCAGGACTAC CAGGCAATAA AGGACAACCT GGCTTTCCAG GTCAAACAGG AAGCAAAGGG 
ATTCAAGGAC CCCAAGGATT CCCTGGACTC CCAGGAACAC AAGGCCAAAT GGGAATTCCT 
GGTGTAAAAG GGGAAGAAGG AAACATGGGT CCACCTGGGC CTAGTGGAGA ATGTGGGGAT 
ATGGGAATGA AAGGTGAAAG AGGGCTTCCA GGAGGTGGTG GCAGCATTAA CATTCGGCTT 
GAGAAAGGAC AAAAAGGAGA ACCAGGGTTT CCTGGTGAGA ATGGATTTAG AGGTGAAAGA 
GGTGAAAAAG GAAATACAGG TTTCAGAGGT GCCCCAGGAT TTCCAGGTAA CAACGGTGCC 
CCAGGACTTC CAGGTGACCA TGGTGACATT GGACTGATGG GATTTCCAGG ATCACGTGGT 
TTCCCAGGAC CAAAGGGCTC TAAGGGAGTT ACAGGTTTCA AAGGACTACC AGGTGACCAG 
GGTGATGTTG GCTCACCAGG AATGCCTGGA AACCCAGGAC TGACAGGCCC CAAAGGACCT 
AAAGGAAAGA GAGGTGACCC AACTCTTCTG CTTGGTACAC AAGGTCAGAA AGGGCCTCCT 
GGAGATCTAG GATTACCAGG ATTCTGTGGA TTCCCAGGTG AGAAAGGTTC ACCAGGTATC 
CGAGGGCAAC CTGGAAGACC AGGATCCAAG GGCGAGCCTG GATACCCAGG AATACCTGGA 
TTTCCAGGAG CTACAGGTCC TCCAGGATTG CCAGGAGAAC CTGGAGAAAA AGGGAAACAT 
GGAATTTTGG GACCTCCAGG GCTGCTAGGA TTAACTGGAT CACCAGGTAG AAAAGGTCTT 
CCTGGACTGC CTGGACTGGA TGGCTTGGAC GGACTAAAAG GTCAAAAAGG TTCTGCAGGA 
GCTCCAGGTC AAAGTGAAAC AGGGCGCCCA GGATACTCTG GAGAACCTGG ACCAAAAGGA 
GAGAGAGGTG AACCTGGATG GCCTGGTATT TCCATTCCAG GCACCCCTGG AGAAAGAGGA 
TTCCCAGGAT TCCCAGGTAG AAGAGGACCT GTTGGACCCA TTGGACCTAG GGGACCATCT 
CCCAGTAGTG CTCCTCCTGG TCTTCCAGGT GATCAAGGAC CACCTGGTTT AGATGGCATC 
AGAGGTCAGC CTGGGAATCC TGGTCCTCCT GGAGAGACTG TCTTTGTACG AGGAGATCCA 
GGTGACACCG GTATTCGAGG GGCACCAGGC ATTCCTGGAC ACCGAGGGCT ACAAGGAGCC 
AGAGGTCTGC CAGGAAATCA AGGAAGAGAA GGTCCAAAGG GTCCAATGGG AATCCATGGC 
TTACAGGGTC CACCTGGTGC TGTAGGACAA CCAGGAGACC AAGGTTTTCA AGGAAAACCT 
GGTCCTAAAG GTCCCACAGG TGAGCCTGGT GAACCAGGAA AAACAGATGA CTCATGTCCT 
GCAATCCCAG GGCCTCCAGG GGAAGCAGGA CATAGAGGAG ATGATGGTTC TATAGGATTA 
CCAGGGCCAA TAGGTCACCC TGGACCCCAA GGAAGGAAAG GTGAAGAAGG GTCATGTGGC 
CTGCCAGGTC AAGATGGCTT ACCAGGAGCA CCTGGACCAC CAGGTGACCA AGGGCATGTG 
GGAGAACAAG GATATGCTGG ACCACAAGGT CCACCTGGCC AGACTGGTAT CCCAGGACCA 
CCAGGGCCCC ATGTTAGATC TTCAAGTGGA TTCTTGCTCG TCCTTCATAG TCAGTCAGAC 
AGAGAACCAC TTTGTCCACA GGGGATGCCA AAATTATGGA CTGGCTATAG TCTGCTGTAC 
CTGGAAGGAC AAGAGAAAGC TCATAATCAA GATCTTGGTC TGGCAGGATC TTGTCTTCCT 
GTATTTAATA CCATGCCCTT TGCTTACTGT AACATCAACC AAGTTTGTTA CTATGCCAGC 
CGAAATGATA AGTCCTACTG GTTGTCAAGT GCCGCTCCCT TACCAATGAC ACCACTGTCT 
GAGGAAGAAA TACAACCATA TATAAGCAGA TGTGCTGTAT GTGAGGCCCC AGCCCAAGCA 
GTAGCAGTTC ACAGCCAAGA TCAGTCAATT CCTCCCTGCC CAATGAACTG GAGAAGTCTT 
TGGATAGGAT ATTCTTTTCT GATGCACACT GGATCTGGTG ATCAGGGAGG TGGGCAGTCC 
CTTATGTCAC CAGGAAGCTG CTTAGAAGAC TTCAGATCAG CACCGTTCAT TGAATGCCAG 
GGTCAGCGGG GAACTTGTCA GTTCTTTGCT AATGAATACA GCTTTTGGCT GACAACAGTG 
ATGCCTGACT TGCAGTTTGC ATCAGCCCCC TTGTCAGGAA CTCTTAAAGA AGGACAAGAG 
CAGAGGAAAA AAATCAGTAG ATGCCAGGTA TGCCTGAAGC ATGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025009445.1
CDS	276..5303
Translation	MALTKDSFERIKWLIRTAWWLLIVFSAQEIDGGGYAYIDPCGGQDCSVCRCFPEKGSRGQPGELGAQGPIGSLGPTGPAGLPGEKGQRGENGQPGPAGEKGDKGPTGVPGFPGLDGVPGLPGSEGPRGKHGVDGCNGSRGDPGFPGENGYIGPRGPYGNPGQKGEKGNSVYVSYYGKGPPGDRGDPGPPGMPGPRGSAGTTGPPGYPGQPGMPGIPGYPGLPGEQGNPGIGVDGQKGEPGDVGLPGPPGSPLLVGPPGAQLFKGEKGQKGLPGITGYRGPHGPKGIPGKGEKGEKGIPGFPGLRGSPGSHGPAGFPGIKGEMGVAGFPGKPGYPGIKGDPGEKGLPGPPGTPLLPMKGPPGDPGFPGPAGDMGSVGPSGPVGMLGRPGDDGTSLPGLPGISGAPGPQGFQGDPGLPGTGEAIPGRPGFPGPPGLPGQPGRQGLPGLPSVICTERGIPGEPGAKGQIGLPGRKGEKGEKGNQGLCSCSAGPPGPRGIQGPSGTQGKKGQMGYPGRRGEKGDSGLSGAVGSPGLPGTPGSGGQHGEKGEKGDQGRVSIKGIKGERGPTGVPGFPGQKGNDGRDGDLGLPGEKGDEGDSGVPIPGDKGFPGVPGLPGVKGPMGLPGLGFPGPPGVRGTPGDFGDDGNIGLPGPKGQKGDTICITSPYPGNPGPPGFKGVQGPKGLKGLPGLPGRSGFDGPKGQQGRPGAGIPGPEGFRGTAGDPGDEGERGSTIDGKNGAPGPPGIDGQKGVSGDTTYGPPGFPGNRGLPGPPGAQGARGDPGIPGLQGHPGTPGFPGAKGFRGPEGDTGAPGCPGTPGPPCDTQLPGPPGLRGVIGLPGSQGLPGFKGQKGDRGLAGPPGIKGLKGSPGSQGPPGPPGSRGLPGLPGNKGQPGFPGQTGSKGIQGPQGFPGLPGTQGQMGIPGVKGEEGNMGPPGPSGECGDMGMKGERGLPGGGGSINIRLEKGQKGEPGFPGENGFRGERGEKGNTGFRGAPGFPGNNGAPGLPGDHGDIGLMGFPGSRGFPGPKGSKGVTGFKGLPGDQGDVGSPGMPGNPGLTGPKGPKGKRGDPTLLLGTQGQKGPPGDLGLPGFCGFPGEKGSPGIRGQPGRPGSKGEPGYPGIPGFPGATGPPGLPGEPGEKGKHGILGPPGLLGLTGSPGRKGLPGLPGLDGLDGLKGQKGSAGAPGQSETGRPGYSGEPGPKGERGEPGWPGISIPGTPGERGFPGFPGRRGPVGPIGPRGPSPSSAPPGLPGDQGPPGLDGIRGQPGNPGPPGETVFVRGDPGDTGIRGAPGIPGHRGLQGARGLPGNQGREGPKGPMGIHGLQGPPGAVGQPGDQGFQGKPGPKGPTGEPGEPGKTDDSCPAIPGPPGEAGHRGDDGSIGLPGPIGHPGPQGRKGEEGSCGLPGQDGLPGAPGPPGDQGHVGEQGYAGPQGPPGQTGIPGPPGPHVRSSSGFLLVLHSQSDREPLCPQGMPKLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFNTMPFAYCNINQVCYYASRNDKSYWLSSAAPLPMTPLSEEEIQPYISRCAVCEAPAQAVAVHSQDQSIPPCPMNWRSLWIGYSFLMHTGSGDQGGGQSLMSPGSCLEDFRSAPFIECQGQRGTCQFFANEYSFWLTTVMPDLQFASAPLSGTLKEGQEQRKKISRCQVCLKHD

Target ORF information:

RefSeq Version	XM_025153677.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus collagen type IV alpha 4 chain (COL4A4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025153677.1

ORF Insert Sequence:

ATGGCGTTAA CAAAGGATTC ATTTGAACGA ATTAAGTGGC TTATCAGAAC TGCCTGGTGG 
CTGCTGATTG TTTTTTCAGC ACAAGAAATT GATGGGGGAG GATACGCATA TATTGACCCA 
TGTGGGGGAC AGGATTGTTC GGTTTGTCGG TGCTTTCCAG AAAAAGGATC AAGAGGTCAA 
CCTGGTGAGC TGGGAGCTCA AGGTCCAATT GGATCGTTAG GACCTACAGG ACCAGCTGGT 
CTGCCAGGGG AAAAAGGACA GAGAGGTGAG AATGGGCAGC CTGGGCCAGC TGGAGAAAAA 
GGTGATAAGG GCCCAACTGG AGTCCCAGGA TTTCCTGGTT TAGATGGTGT TCCTGGATTG 
CCAGGAAGTG AAGGACCCAG GGGCAAGCAC GGTGTAGATG GCTGCAATGG CTCAAGAGGA 
GATCCAGGAT TTCCAGGCGA AAATGGATAT ATTGGACCAA GAGGTCCTTA CGGAAACCCA 
GGGCAAAAAG GAGAAAAAGG AAATTCAGTG TATGTTTCGT ATTATGGAAA AGGACCCCCA 
GGAGACAGAG GTGATCCTGG CCCACCTGGT ATGCCCGGAC CTAGAGGATC GGCAGGTACA 
ACAGGCCCAC CTGGATACCC AGGCCAACCA GGCATGCCCG GTATTCCAGG TTACCCTGGT 
TTGCCAGGTG AACAGGGAAA TCCAGGTATT GGAGTGGATG GACAAAAGGG GGAGCCGGGT 
GATGTTGGAC TTCCCGGGCC ACCTGGGTCA CCACTGTTAG TTGGACCTCC TGGAGCTCAG 
CTGTTCAAAG GAGAAAAGGG CCAAAAAGGA CTACCTGGGA TAACGGGATA CAGAGGACCA 
CATGGACCTA AAGGAATACC TGGGAAAGGA GAAAAGGGTG AAAAAGGCAT TCCTGGATTC 
CCTGGATTGC GGGGTAGCCC TGGTTCTCAT GGACCTGCAG GCTTTCCTGG AATAAAGGGA 
GAAATGGGTG TTGCTGGTTT TCCTGGGAAA CCTGGCTATC CAGGCATCAA GGGGGATCCT 
GGAGAAAAAG GGCTCCCCGG TCCTCCTGGA ACTCCACTGC TTCCTATGAA AGGCCCACCA 
GGAGATCCTG GATTTCCAGG TCCTGCGGGT GACATGGGGT CAGTTGGACC TTCTGGCCCT 
GTGGGTATGC TAGGAAGACC AGGAGACGAT GGAACAAGTC TGCCTGGCCT GCCAGGAATA 
AGTGGGGCAC CAGGACCTCA AGGTTTTCAG GGTGACCCTG GTTTGCCTGG AACAGGTGAA 
GCAATTCCAG GAAGACCTGG ATTTCCTGGA CCACCTGGAC TACCAGGACA GCCAGGAAGA 
CAAGGCTTAC CTGGACTACC TAGTGTAATT TGTACTGAGA GAGGGATTCC TGGGGAACCT 
GGAGCAAAAG GTCAGATAGG CCTTCCGGGT AGAAAAGGTG AAAAAGGAGA AAAAGGAAAT 
CAAGGCCTCT GCTCATGTAG TGCTGGTCCT CCTGGTCCCC GTGGTATACA AGGACCTTCA 
GGTACTCAAG GGAAGAAAGG ACAAATGGGT TACCCTGGAA GACGTGGAGA AAAGGGTGAT 
TCAGGCTTAT CTGGTGCAGT GGGATCACCA GGACTCCCTG GGACACCTGG TTCTGGAGGA 
CAACATGGTG AAAAAGGAGA GAAGGGTGAC CAGGGAAGAG TTTCAATAAA AGGAATAAAA 
GGTGAAAGAG GTCCTACTGG GGTTCCAGGA TTTCCAGGCC AAAAAGGTAA TGATGGCAGA 
GATGGGGATT TAGGATTGCC TGGGGAAAAA GGAGATGAGG GTGATTCTGG AGTACCAATA 
CCAGGTGATA AAGGATTTCC TGGAGTTCCA GGACTCCCTG GAGTAAAAGG ACCAATGGGA 
TTGCCTGGTT TGGGGTTTCC TGGCCCTCCG GGAGTTAGAG GTACCCCTGG AGACTTTGGT 
GATGATGGTA ACATTGGTCT GCCTGGTCCA AAGGGTCAGA AAGGTGACAC TATCTGTATT 
ACATCACCGT ACCCTGGAAA CCCAGGTCCC CCAGGTTTTA AAGGTGTTCA AGGACCAAAG 
GGTTTAAAAG GACTCCCTGG TCTTCCTGGA CGAAGTGGCT TTGATGGGCC AAAAGGCCAG 
CAAGGCAGGC CAGGAGCAGG AATCCCTGGA CCAGAAGGCT TCCGAGGCAC AGCTGGTGAT 
CCTGGTGATG AAGGTGAAAG AGGATCTACC ATTGATGGTA AAAATGGTGC TCCAGGTCCA 
CCTGGCATAG ATGGCCAGAA GGGTGTTTCA GGTGATACTA CATATGGCCC TCCAGGATTC 
CCAGGCAACA GGGGACTGCC AGGACCACCT GGTGCACAAG GAGCAAGGGG TGATCCAGGC 
ATTCCTGGGC TTCAAGGGCA TCCTGGGACC CCAGGATTTC CAGGTGCTAA AGGTTTTAGA 
GGTCCAGAAG GTGATACAGG AGCGCCAGGC TGTCCAGGCA CCCCTGGTCC ACCATGTGAC 
ACACAATTAC CAGGGCCACC TGGACTCAGA GGTGTCATAG GCCTGCCAGG TTCTCAAGGC 
TTACCAGGCT TTAAAGGTCA GAAAGGAGAC AGGGGCCTAG CTGGGCCACC TGGAATCAAG 
GGTCTCAAGG GCTCTCCTGG CTCACAGGGC CCCCCAGGTC CTCCAGGGTC CCGAGGACTT 
CCAGGACTAC CAGGCAATAA AGGACAACCT GGCTTTCCAG GTCAAACAGG AAGCAAAGGG 
ATTCAAGGAC CCCAAGGATT CCCTGGACTC CCAGGAACAC AAGGCCAAAT GGGAATTCCT 
GGTGTAAAAG GGGAAGAAGG AAACATGGGT CCACCTGGGC CTAGTGGAGA ATGTGGGGAT 
ATGGGAATGA AAGGTGAAAG AGGGCTTCCA GGAGGTGGTG GCAGCATTAA CATTCGGCTT 
GAGAAAGGAC AAAAAGGAGA ACCAGGGTTT CCTGGTGAGA ATGGATTTAG AGGTGAAAGA 
GGTGAAAAAG GAAATACAGG TTTCAGAGGT GCCCCAGGAT TTCCAGGTAA CAACGGTGCC 
CCAGGACTTC CAGGTGACCA TGGTGACATT GGACTGATGG GATTTCCAGG ATCACGTGGT 
TTCCCAGGAC CAAAGGGCTC TAAGGGAGTT ACAGGTTTCA AAGGACTACC AGGTGACCAG 
GGTGATGTTG GCTCACCAGG AATGCCTGGA AACCCAGGAC TGACAGGCCC CAAAGGACCT 
AAAGGAAAGA GAGGTGACCC AACTCTTCTG CTTGGTACAC AAGGTCAGAA AGGGCCTCCT 
GGAGATCTAG GATTACCAGG ATTCTGTGGA TTCCCAGGTG AGAAAGGTTC ACCAGGTATC 
CGAGGGCAAC CTGGAAGACC AGGATCCAAG GGCGAGCCTG GATACCCAGG AATACCTGGA 
TTTCCAGGAG CTACAGGTCC TCCAGGATTG CCAGGAGAAC CTGGAGAAAA AGGGAAACAT 
GGAATTTTGG GACCTCCAGG GCTGCTAGGA TTAACTGGAT CACCAGGTAG AAAAGGTCTT 
CCTGGACTGC CTGGACTGGA TGGCTTGGAC GGACTAAAAG GTCAAAAAGG TTCTGCAGGA 
GCTCCAGGTC AAAGTGAAAC AGGGCGCCCA GGATACTCTG GAGAACCTGG ACCAAAAGGA 
GAGAGAGGTG AACCTGGATG GCCTGGTATT TCCATTCCAG GCACCCCTGG AGAAAGAGGA 
TTCCCAGGAT TCCCAGGTAG AAGAGGACCT GTTGGACCCA TTGGACCTAG GGGACCATCT 
CCCAGTAGTG CTCCTCCTGG TCTTCCAGGT GATCAAGGAC CACCTGGTTT AGATGGCATC 
AGAGGTCAGC CTGGGAATCC TGGTCCTCCT GGAGAGACTG TCTTTGTACG AGGAGATCCA 
GGTGACACCG GTATTCGAGG GGCACCAGGC ATTCCTGGAC ACCGAGGGCT ACAAGGAGCC 
AGAGGTCTGC CAGGAAATCA AGGAAGAGAA GGTCCAAAGG GTCCAATGGG AATCCATGGC 
TTACAGGGTC CACCTGGTGC TGTAGGACAA CCAGGAGACC AAGGTTTTCA AGGAAAACCT 
GGTCCTAAAG GTCCCACAGG TGAGCCTGGT GAACCAGGAA AAACAGATGA CTCATGTCCT 
GCAATCCCAG GGCCTCCAGG GGAAGCAGGA CATAGAGGAG ATGATGGTTC TATAGGATTA 
CCAGGGCCAA TAGGTCACCC TGGACCCCAA GGAAGGAAAG GTGAAGAAGG GTCATGTGGC 
CTGCCAGGTC AAGATGGCTT ACCAGGAGCA CCTGGACCAC CAGGTGACCA AGGGCATGTG 
GGAGAACAAG GATATGCTGG ACCACAAGGT CCACCTGGCC AGACTGGTAT CCCAGGACCA 
CCAGGGCCCC ATGTTAGATC TTCAAGTGGA TTCTTGCTCG TCCTTCATAG TCAGTCAGAC 
AGAGAACCAC TTTGTCCACA GGGGATGCCA AAATTATGGA CTGGCTATAG TCTGCTGTAC 
CTGGAAGGAC AAGAGAAAGC TCATAATCAA GATCTTGGTC TGGCAGGATC TTGTCTTCCT 
GTATTTAATA CCATGCCCTT TGCTTACTGT AACATCAACC AAGTTTGTTA CTATGCCAGC 
CGAAATGATA AGTCCTACTG GTTGTCAAGT GCCGCTCCCT TACCAATGAC ACCACTGTCT 
GAGGAAGAAA TACAACCATA TATAAGCAGA TGTGCTGTAT GTGAGGCCCC AGCCCAAGCA 
GTAGCAGTTC ACAGCCAAGA TCAGTCAATT CCTCCCTGCC CAATGAACTG GAGAAGTCTT 
TGGATAGGAT ATTCTTTTCT GATGCACACT GGATCTGGTG ATCAGGGAGG TGGGCAGTCC 
CTTATGTCAC CAGGAAGCTG CTTAGAAGAC TTCAGATCAG CACCGTTCAT TGAATGCCAG 
GGTCAGCGGG GAACTTGTCA GTTCTTTGCT AATGAATACA GCTTTTGGCT GACAACAGTG 
ATGCCTGACT TGCAGTTTGC ATCAGCCCCC TTGTCAGGAA CTCTTAAAGA AGGACAAGAG 
CAGAGGAAAA AAATCAGTAG ATGCCAGGTA TGCCTGAAGC ATGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_015276986.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015132472.1 Gallus gallus collagen, type IV, alpha 4 (COL4A4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa39728
Clone ID Related Accession (Same CDS sequence)	XM_015276986.1 , XM_015276987.1 , XM_015276987.2 , XM_015276986.2
Accession Version	XM_015276986.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	collagen alpha-4(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471743.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTTAA CAAAGGATTC ATTTGAACGA ATTAAGTGGC TTATCAGAAC TGCCTGGTGG 
CTGCTGATTG TTTTTTCAGC ACAAGAAATT GATGGGGGAG GATACGCATA TATTGACCCA 
TGTGGGGGAC AGGATTGTTC GGTTTGTCGG TGCTTTCCAG AAAAAGGATC AAGAGGTCAA 
CCTGGTGAGC TGGGAGCTCA AGGTCCAATT GGATCGTTAG GACCTACAGG ACCAGCTGGT 
CTGCCAGGGG AAAAAGGACA GAGAGGTGAG AATGGGCAGC CTGGGCCAGC TGGAGAAAAA 
GGTGATAAGG GCCCAACTGG AGTCCCAGGA TTTCCTGGTT TAGATGGTGT TCCTGGATTG 
CCAGGAAGTG AAGGACCCAG GGGCAAGCAC GGTGTAGATG GCTGCAATGG CTCAAGAGGA 
GATCCAGGAT TTCCAGGCGA AAATGGATAT ATTGGACCAA GAGGTCCTTA CGGAAACCCA 
GGGCAAAAAG GAGAAAAAGG AAATTCAGTG TATGTTTCGT ATTATGGAAA AGGACCCCCA 
GGAGACAGAG GTGATCCTGG CCCACCTGGT ATGCCCGGAC CTAGAGGATC GGCAGGTACA 
ACAGGCCCAC CTGGATACCC AGGCCAACCA GGCATGCCCG GTATTCCAGG TTACCCTGGT 
TTGCCAGGTG AACAGGGAAA TCCAGGTATT GGAGTGGATG GACAAAAGGG GGAGCCGGGT 
GATGTTGGAC TTCCCGGGCC ACCTGGGTCA CCACTGTTAG TTGGACCTCC TGGAGCTCAG 
CTGTTCAAAG GAGAAAAGGG CCAAAAAGGA CTACCTGGGA TAACGGGATA CAGAGGACCA 
CATGGACCTA AAGGAATACC TGGGAAAGGA GAAAAGGGTG AAAAAGGCAT TCCTGGATTC 
CCTGGATTGC GGGGTAGCCC TGGTTCTCAT GGACCTGCAG GCTTTCCTGG AATAAAGGGA 
GAAATGGGTG TTGCTGGTTT TCCTGGGAAA CCTGGCTATC CAGGCATCAA GGGGGATCCT 
GGAGAAAAAG GGCTCCCCGG TCCTCCTGGA ACTCCACTGC TTCCTATGAA AGGCCCACCA 
GGAGATCCTG GATTTCCAGG TCCTGCGGGT GACATGGGGT CAGTTGGACC TTCTGGCCCT 
GTGGGTATGC TAGGAAGACC AGGAGACGAT GGAACAAGTC TGCCTGGCCT GCCAGGAATA 
AGTGGGGCAC CAGGACCTCA AGGTTTTCAG GGTGACCCTG GTTTGCCTGG AACAGGTGAA 
GCAATTCCAG GAAGACCTGG ATTTCCTGGA CCACCTGGAC TACCAGGACA GCCAGGAAGA 
CAAGGCTTAC CTGGACTACC TAGTGTAATT TGTACTGAGA GAGGGATTCC TGGGGAACCT 
GGAGCAAAAG GTCAGATAGG CCTTCCGGGT AGAAAAGGTG AAAAAGGAGA AAAAGGAAAT 
CAAGGCCTCT GCTCATGTAG TGCTGGTCCT CCTGGTCCCC GTGGTATACA AGGACCTTCA 
GGTACTCAAG GGAAGAAAGG ACAAATGGGT TACCCTGGAA GACGTGGAGA AAAGGGTGAT 
TCAGGCTTAT CTGGTGCAGT GGGATCACCA GGACTCCCTG GGACACCTGG TTCTGGAGGA 
CAACATGGTG AAAAAGGAGA GAAGGGTGAC CAGGGAAGAG TTTCAATAAA AGGAATAAAA 
GGTGAAAGAG GTCCTACTGG GGTTCCAGGA TTTCCAGGCC AAAAAGGTAA TGATGGCAGA 
GATGGGGATT TAGGATTGCC TGGGGAAAAA GGAGATGAGG GTGATTCTGG AGTACCAATA 
CCAGGTGATA AAGGATTTCC TGGAGTTCCA GGACTCCCTG GAGTAAAAGG ACCAATGGGA 
TTGCCTGGTT TGGGGTTTCC TGGCCCTCCG GGAGTTAGAG GTACCCCTGG AGACTTTGGT 
GATGATGGTA ACATTGGTCT GCCTGGTCCA AAGGGTCAGA AAGGTGACAC TATCTGTATT 
ACATCACCGT ACCCTGGAAA CCCAGGTCCC CCAGGTTTTA AAGGTGTTCA AGGACCAAAG 
GGTTTAAAAG GACTCCCTGG TCTTCCTGGA CGAAGTGGCT TTGATGGGCC AAAAGGCCAG 
CAAGGCAGGC CAGGAGCAGG AATCCCTGGA CCAGAAGGCT TCCGAGGCAC AGCTGGTGAT 
CCTGGTGATG AAGGTGAAAG AGGATCTACC ATTGATGGTA AAAATGGTGC TCCAGGTCCA 
CCTGGCATAG ATGGCCAGAA GGGTGTTTCA GGTGATACTA CATATGGCCC TCCAGGATTC 
CCAGGCAACA GGGGACTGCC AGGACCACCT GGTGCACAAG GAGCAAGGGG TGATCCAGGC 
ATTCCTGGGC TTCAAGGGCA TCCTGGGACC CCAGGATTTC CAGGTGCTAA AGGTTTTAGA 
GGTCCAGAAG GTGATACAGG AGCGCCAGGC TGTCCAGGCA CCCCTGGTCC ACCATGTGAC 
ACACAATTAC CAGGGCCACC TGGACTCAGA GGTGTCATAG GCCTGCCAGG TTCTCAAGGC 
TTACCAGGCT TTAAAGGTCA GAAAGGAGAC AGGGGCCTAG CTGGGCCACC TGGAATCAAG 
GGTCTCAAGG GCTCTCCTGG CTCACAGGGC CCCCCAGGTC CTCCAGGGTC CCGAGGACTT 
CCAGGACTAC CAGGCAATAA AGGACAACCT GGCTTTCCAG GTCAAACAGG AAGCAAAGGG 
ATTCAAGGAC CCCAAGGATT CCCTGGACTC CCAGGAACAC AAGGCCAAAT GGGAATTCCT 
GGTGTAAAAG GGGAAGAAGG AAACATGGGT CCACCTGGGC CTAGTGGAGA ATGTGGGGAT 
ATGGGAATGA AAGGTGAAAG AGGGCTTCCA GGAGGTGGTG GCAGCATTAA CATTCGGCTT 
GAGAAAGGAC AAAAAGGAGA ACCAGGGTTT CCTGGTGAGA ATGGATTTAG AGGTGAAAGA 
GGTGAAAAAG GAAATACAGG TTTCAGAGGT GCCCCAGGAT TTCCAGGTAA CAACGGTGCC 
CCAGGACTTC CAGGTGACCA TGGTGACATT GGACTGATGG GATTTCCAGG ATCACGTGGT 
TTCCCAGGAC CAAAGGGCTC TAAGGGAGTT ACAGGTTTCA AAGGACTACC AGGTGACCAG 
GGTGATGTTG GCTCACCAGG AATGCCTGGA AACCCAGGAC TGACAGGCCC CAAAGGACCT 
AAAGGAAAGA GAGGTGACCC AACTCTTCTG CTTGGTACAC AAGGTCAGAA AGGGCCTCCT 
GGAGATCTAG GATTACCAGG ATTCTGTGGA TTCCCAGGTG AGAAAGGTTC ACCAGGTATC 
CGAGGGCAAC CTGGAAGACC AGGATCCAAG GGCGAGCCTG GATACCCAGG AATACCTGGA 
TTTCCAGGAG CTACAGGTCC TCCAGGATTG CCAGGAGAAC CTGGAGAAAA AGGGAAACAT 
GGAATTTTGG GACCTCCAGG GCTGCTAGGA TTAACTGGAT CACCAGGTAG AAAAGGTCTT 
CCTGGACTGC CTGGACTGGA TGGCTTGGAC GGACTAAAAG GTCAAAAAGG TTCTGCAGGA 
GCTCCAGGTC AAAGTGAAAC AGGGCGCCCA GGATACTCTG GAGAACCTGG ACCAAAAGGA 
GAGAGAGGTG AACCTGGATG GCCTGGTATT TCCATTCCAG GCACCCCTGG AGAAAGAGGA 
TTCCCAGGAT TCCCAGGTAG AAGAGGACCT GTTGGACCCA TTGGACCTAG GGGACCATCT 
CCCAGTAGTG CTCCTCCTGG TCTTCCAGGT GATCAAGGAC CACCTGGTTT AGATGGCATC 
AGAGGTCAGC CTGGGAATCC TGGTCCTCCT GGAGAGACTG TCTTTGTACG AGGAGATCCA 
GGTGACACCG GTATTCGAGG GGCACCAGGC ATTCCTGGAC ACCGAGGGCT ACAAGGAGCC 
AGAGGTCTGC CAGGAAATCA AGGAAGAGAA GGTCCAAAGG GTCCAATGGG AATCCATGGC 
TTACAGGGTC CACCTGGTGC TGTAGGACAA CCAGGAGACC AAGGTTTTCA AGGAAAACCT 
GGTCCTAAAG GTCCCACAGG GTTCATAGGT GAGCCTGGTG AACCAGGAAA AACAGATGAC 
TCATGTCCTG CAATCCCAGG GCCTCCAGGG GAAGCAGGAC ATAGAGGAGA TGATGGTTCT 
ATAGGATTAC CAGGGCCAAT AGGTCACCCT GGACCCCAAG GAAGGAAAGG TGAAGAAGGG 
TCATGTGGCC TGCCAGGTCA AGATGGCTTA CCAGGAGCAC CTGGACCACC AGGTGACCAA 
GGGCATGTGG GAGAACAAGG ATATGCTGGA CCACAAGGTC CACCTGGCCA GACTGGTATC 
CCAGGACCAC CAGGGCCCCA TGTTAGATCT TCAAGTGGAT TCTTGCTCGT CCTTCATAGT 
CAGTCAGACA GAGAACCACT TTGTCCACAG GGGATGCCAA AATTATGGAC TGGCTATAGT 
CTGCTGTACC TGGAAGGACA AGAGAAAGCT CATAATCAAG ATCTTGGTCT GGCAGGATCT 
TGTCTTCCTG TATTTAATAC CATGCCCTTT GCTTACTGTA ACATCAACCA AGTTTGTTAC 
TATGCCAGCC GAAATGATAA GTCCTACTGG TTGTCAAGTG CCGCTCCCTT ACCAATGACA 
CCACTGTCTG AGGAAGAAAT ACAACCATAT ATAAGCAGAT GTGCTGTATG TGAGGCCCCA 
GCCCAAGCAG TAGCAGTTCA CAGCCAAGAT CAGTCAATTC CTCCCTGCCC AATGAACTGG 
AGAAGTCTTT GGATAGGATA TTCTTTTCTG ATGCACACTG GATCTGGTGA TCAGGGAGGT 
GGGCAGTCCC TTATGTCACC AGGAAGCTGC TTAGAAGACT TCAGATCAGC ACCGTTCATT 
GAATGCCAGG GTCAGCGGGG AACTTGTCAG TTCTTTGCTA ATGAATACAG CTTTTGGCTG 
ACAACAGTGA TGCCTGACTT GCAGTTTGCA TCAGCCCCCT TGTCAGGAAC TCTTAAAGAA 
GGACAAGAGC AGAGGAAAAA AATCAGTAGA TGCCAGGTAT GCCTGAAGCA TGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015132472.1
CDS	237..5273
Translation	MALTKDSFERIKWLIRTAWWLLIVFSAQEIDGGGYAYIDPCGGQDCSVCRCFPEKGSRGQPGELGAQGPIGSLGPTGPAGLPGEKGQRGENGQPGPAGEKGDKGPTGVPGFPGLDGVPGLPGSEGPRGKHGVDGCNGSRGDPGFPGENGYIGPRGPYGNPGQKGEKGNSVYVSYYGKGPPGDRGDPGPPGMPGPRGSAGTTGPPGYPGQPGMPGIPGYPGLPGEQGNPGIGVDGQKGEPGDVGLPGPPGSPLLVGPPGAQLFKGEKGQKGLPGITGYRGPHGPKGIPGKGEKGEKGIPGFPGLRGSPGSHGPAGFPGIKGEMGVAGFPGKPGYPGIKGDPGEKGLPGPPGTPLLPMKGPPGDPGFPGPAGDMGSVGPSGPVGMLGRPGDDGTSLPGLPGISGAPGPQGFQGDPGLPGTGEAIPGRPGFPGPPGLPGQPGRQGLPGLPSVICTERGIPGEPGAKGQIGLPGRKGEKGEKGNQGLCSCSAGPPGPRGIQGPSGTQGKKGQMGYPGRRGEKGDSGLSGAVGSPGLPGTPGSGGQHGEKGEKGDQGRVSIKGIKGERGPTGVPGFPGQKGNDGRDGDLGLPGEKGDEGDSGVPIPGDKGFPGVPGLPGVKGPMGLPGLGFPGPPGVRGTPGDFGDDGNIGLPGPKGQKGDTICITSPYPGNPGPPGFKGVQGPKGLKGLPGLPGRSGFDGPKGQQGRPGAGIPGPEGFRGTAGDPGDEGERGSTIDGKNGAPGPPGIDGQKGVSGDTTYGPPGFPGNRGLPGPPGAQGARGDPGIPGLQGHPGTPGFPGAKGFRGPEGDTGAPGCPGTPGPPCDTQLPGPPGLRGVIGLPGSQGLPGFKGQKGDRGLAGPPGIKGLKGSPGSQGPPGPPGSRGLPGLPGNKGQPGFPGQTGSKGIQGPQGFPGLPGTQGQMGIPGVKGEEGNMGPPGPSGECGDMGMKGERGLPGGGGSINIRLEKGQKGEPGFPGENGFRGERGEKGNTGFRGAPGFPGNNGAPGLPGDHGDIGLMGFPGSRGFPGPKGSKGVTGFKGLPGDQGDVGSPGMPGNPGLTGPKGPKGKRGDPTLLLGTQGQKGPPGDLGLPGFCGFPGEKGSPGIRGQPGRPGSKGEPGYPGIPGFPGATGPPGLPGEPGEKGKHGILGPPGLLGLTGSPGRKGLPGLPGLDGLDGLKGQKGSAGAPGQSETGRPGYSGEPGPKGERGEPGWPGISIPGTPGERGFPGFPGRRGPVGPIGPRGPSPSSAPPGLPGDQGPPGLDGIRGQPGNPGPPGETVFVRGDPGDTGIRGAPGIPGHRGLQGARGLPGNQGREGPKGPMGIHGLQGPPGAVGQPGDQGFQGKPGPKGPTGFIGEPGEPGKTDDSCPAIPGPPGEAGHRGDDGSIGLPGPIGHPGPQGRKGEEGSCGLPGQDGLPGAPGPPGDQGHVGEQGYAGPQGPPGQTGIPGPPGPHVRSSSGFLLVLHSQSDREPLCPQGMPKLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFNTMPFAYCNINQVCYYASRNDKSYWLSSAAPLPMTPLSEEEIQPYISRCAVCEAPAQAVAVHSQDQSIPPCPMNWRSLWIGYSFLMHTGSGDQGGGQSLMSPGSCLEDFRSAPFIECQGQRGTCQFFANEYSFWLTTVMPDLQFASAPLSGTLKEGQEQRKKISRCQVCLKHD

Target ORF information:

RefSeq Version	XM_015276986.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus collagen, type IV, alpha 4 (COL4A4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276986.1

ORF Insert Sequence:

ATGGCGTTAA CAAAGGATTC ATTTGAACGA ATTAAGTGGC TTATCAGAAC TGCCTGGTGG 
CTGCTGATTG TTTTTTCAGC ACAAGAAATT GATGGGGGAG GATACGCATA TATTGACCCA 
TGTGGGGGAC AGGATTGTTC GGTTTGTCGG TGCTTTCCAG AAAAAGGATC AAGAGGTCAA 
CCTGGTGAGC TGGGAGCTCA AGGTCCAATT GGATCGTTAG GACCTACAGG ACCAGCTGGT 
CTGCCAGGGG AAAAAGGACA GAGAGGTGAG AATGGGCAGC CTGGGCCAGC TGGAGAAAAA 
GGTGATAAGG GCCCAACTGG AGTCCCAGGA TTTCCTGGTT TAGATGGTGT TCCTGGATTG 
CCAGGAAGTG AAGGACCCAG GGGCAAGCAC GGTGTAGATG GCTGCAATGG CTCAAGAGGA 
GATCCAGGAT TTCCAGGCGA AAATGGATAT ATTGGACCAA GAGGTCCTTA CGGAAACCCA 
GGGCAAAAAG GAGAAAAAGG AAATTCAGTG TATGTTTCGT ATTATGGAAA AGGACCCCCA 
GGAGACAGAG GTGATCCTGG CCCACCTGGT ATGCCCGGAC CTAGAGGATC GGCAGGTACA 
ACAGGCCCAC CTGGATACCC AGGCCAACCA GGCATGCCCG GTATTCCAGG TTACCCTGGT 
TTGCCAGGTG AACAGGGAAA TCCAGGTATT GGAGTGGATG GACAAAAGGG GGAGCCGGGT 
GATGTTGGAC TTCCCGGGCC ACCTGGGTCA CCACTGTTAG TTGGACCTCC TGGAGCTCAG 
CTGTTCAAAG GAGAAAAGGG CCAAAAAGGA CTACCTGGGA TAACGGGATA CAGAGGACCA 
CATGGACCTA AAGGAATACC TGGGAAAGGA GAAAAGGGTG AAAAAGGCAT TCCTGGATTC 
CCTGGATTGC GGGGTAGCCC TGGTTCTCAT GGACCTGCAG GCTTTCCTGG AATAAAGGGA 
GAAATGGGTG TTGCTGGTTT TCCTGGGAAA CCTGGCTATC CAGGCATCAA GGGGGATCCT 
GGAGAAAAAG GGCTCCCCGG TCCTCCTGGA ACTCCACTGC TTCCTATGAA AGGCCCACCA 
GGAGATCCTG GATTTCCAGG TCCTGCGGGT GACATGGGGT CAGTTGGACC TTCTGGCCCT 
GTGGGTATGC TAGGAAGACC AGGAGACGAT GGAACAAGTC TGCCTGGCCT GCCAGGAATA 
AGTGGGGCAC CAGGACCTCA AGGTTTTCAG GGTGACCCTG GTTTGCCTGG AACAGGTGAA 
GCAATTCCAG GAAGACCTGG ATTTCCTGGA CCACCTGGAC TACCAGGACA GCCAGGAAGA 
CAAGGCTTAC CTGGACTACC TAGTGTAATT TGTACTGAGA GAGGGATTCC TGGGGAACCT 
GGAGCAAAAG GTCAGATAGG CCTTCCGGGT AGAAAAGGTG AAAAAGGAGA AAAAGGAAAT 
CAAGGCCTCT GCTCATGTAG TGCTGGTCCT CCTGGTCCCC GTGGTATACA AGGACCTTCA 
GGTACTCAAG GGAAGAAAGG ACAAATGGGT TACCCTGGAA GACGTGGAGA AAAGGGTGAT 
TCAGGCTTAT CTGGTGCAGT GGGATCACCA GGACTCCCTG GGACACCTGG TTCTGGAGGA 
CAACATGGTG AAAAAGGAGA GAAGGGTGAC CAGGGAAGAG TTTCAATAAA AGGAATAAAA 
GGTGAAAGAG GTCCTACTGG GGTTCCAGGA TTTCCAGGCC AAAAAGGTAA TGATGGCAGA 
GATGGGGATT TAGGATTGCC TGGGGAAAAA GGAGATGAGG GTGATTCTGG AGTACCAATA 
CCAGGTGATA AAGGATTTCC TGGAGTTCCA GGACTCCCTG GAGTAAAAGG ACCAATGGGA 
TTGCCTGGTT TGGGGTTTCC TGGCCCTCCG GGAGTTAGAG GTACCCCTGG AGACTTTGGT 
GATGATGGTA ACATTGGTCT GCCTGGTCCA AAGGGTCAGA AAGGTGACAC TATCTGTATT 
ACATCACCGT ACCCTGGAAA CCCAGGTCCC CCAGGTTTTA AAGGTGTTCA AGGACCAAAG 
GGTTTAAAAG GACTCCCTGG TCTTCCTGGA CGAAGTGGCT TTGATGGGCC AAAAGGCCAG 
CAAGGCAGGC CAGGAGCAGG AATCCCTGGA CCAGAAGGCT TCCGAGGCAC AGCTGGTGAT 
CCTGGTGATG AAGGTGAAAG AGGATCTACC ATTGATGGTA AAAATGGTGC TCCAGGTCCA 
CCTGGCATAG ATGGCCAGAA GGGTGTTTCA GGTGATACTA CATATGGCCC TCCAGGATTC 
CCAGGCAACA GGGGACTGCC AGGACCACCT GGTGCACAAG GAGCAAGGGG TGATCCAGGC 
ATTCCTGGGC TTCAAGGGCA TCCTGGGACC CCAGGATTTC CAGGTGCTAA AGGTTTTAGA 
GGTCCAGAAG GTGATACAGG AGCGCCAGGC TGTCCAGGCA CCCCTGGTCC ACCATGTGAC 
ACACAATTAC CAGGGCCACC TGGACTCAGA GGTGTCATAG GCCTGCCAGG TTCTCAAGGC 
TTACCAGGCT TTAAAGGTCA GAAAGGAGAC AGGGGCCTAG CTGGGCCACC TGGAATCAAG 
GGTCTCAAGG GCTCTCCTGG CTCACAGGGC CCCCCAGGTC CTCCAGGGTC CCGAGGACTT 
CCAGGACTAC CAGGCAATAA AGGACAACCT GGCTTTCCAG GTCAAACAGG AAGCAAAGGG 
ATTCAAGGAC CCCAAGGATT CCCTGGACTC CCAGGAACAC AAGGCCAAAT GGGAATTCCT 
GGTGTAAAAG GGGAAGAAGG AAACATGGGT CCACCTGGGC CTAGTGGAGA ATGTGGGGAT 
ATGGGAATGA AAGGTGAAAG AGGGCTTCCA GGAGGTGGTG GCAGCATTAA CATTCGGCTT 
GAGAAAGGAC AAAAAGGAGA ACCAGGGTTT CCTGGTGAGA ATGGATTTAG AGGTGAAAGA 
GGTGAAAAAG GAAATACAGG TTTCAGAGGT GCCCCAGGAT TTCCAGGTAA CAACGGTGCC 
CCAGGACTTC CAGGTGACCA TGGTGACATT GGACTGATGG GATTTCCAGG ATCACGTGGT 
TTCCCAGGAC CAAAGGGCTC TAAGGGAGTT ACAGGTTTCA AAGGACTACC AGGTGACCAG 
GGTGATGTTG GCTCACCAGG AATGCCTGGA AACCCAGGAC TGACAGGCCC CAAAGGACCT 
AAAGGAAAGA GAGGTGACCC AACTCTTCTG CTTGGTACAC AAGGTCAGAA AGGGCCTCCT 
GGAGATCTAG GATTACCAGG ATTCTGTGGA TTCCCAGGTG AGAAAGGTTC ACCAGGTATC 
CGAGGGCAAC CTGGAAGACC AGGATCCAAG GGCGAGCCTG GATACCCAGG AATACCTGGA 
TTTCCAGGAG CTACAGGTCC TCCAGGATTG CCAGGAGAAC CTGGAGAAAA AGGGAAACAT 
GGAATTTTGG GACCTCCAGG GCTGCTAGGA TTAACTGGAT CACCAGGTAG AAAAGGTCTT 
CCTGGACTGC CTGGACTGGA TGGCTTGGAC GGACTAAAAG GTCAAAAAGG TTCTGCAGGA 
GCTCCAGGTC AAAGTGAAAC AGGGCGCCCA GGATACTCTG GAGAACCTGG ACCAAAAGGA 
GAGAGAGGTG AACCTGGATG GCCTGGTATT TCCATTCCAG GCACCCCTGG AGAAAGAGGA 
TTCCCAGGAT TCCCAGGTAG AAGAGGACCT GTTGGACCCA TTGGACCTAG GGGACCATCT 
CCCAGTAGTG CTCCTCCTGG TCTTCCAGGT GATCAAGGAC CACCTGGTTT AGATGGCATC 
AGAGGTCAGC CTGGGAATCC TGGTCCTCCT GGAGAGACTG TCTTTGTACG AGGAGATCCA 
GGTGACACCG GTATTCGAGG GGCACCAGGC ATTCCTGGAC ACCGAGGGCT ACAAGGAGCC 
AGAGGTCTGC CAGGAAATCA AGGAAGAGAA GGTCCAAAGG GTCCAATGGG AATCCATGGC 
TTACAGGGTC CACCTGGTGC TGTAGGACAA CCAGGAGACC AAGGTTTTCA AGGAAAACCT 
GGTCCTAAAG GTCCCACAGG GTTCATAGGT GAGCCTGGTG AACCAGGAAA AACAGATGAC 
TCATGTCCTG CAATCCCAGG GCCTCCAGGG GAAGCAGGAC ATAGAGGAGA TGATGGTTCT 
ATAGGATTAC CAGGGCCAAT AGGTCACCCT GGACCCCAAG GAAGGAAAGG TGAAGAAGGG 
TCATGTGGCC TGCCAGGTCA AGATGGCTTA CCAGGAGCAC CTGGACCACC AGGTGACCAA 
GGGCATGTGG GAGAACAAGG ATATGCTGGA CCACAAGGTC CACCTGGCCA GACTGGTATC 
CCAGGACCAC CAGGGCCCCA TGTTAGATCT TCAAGTGGAT TCTTGCTCGT CCTTCATAGT 
CAGTCAGACA GAGAACCACT TTGTCCACAG GGGATGCCAA AATTATGGAC TGGCTATAGT 
CTGCTGTACC TGGAAGGACA AGAGAAAGCT CATAATCAAG ATCTTGGTCT GGCAGGATCT 
TGTCTTCCTG TATTTAATAC CATGCCCTTT GCTTACTGTA ACATCAACCA AGTTTGTTAC 
TATGCCAGCC GAAATGATAA GTCCTACTGG TTGTCAAGTG CCGCTCCCTT ACCAATGACA 
CCACTGTCTG AGGAAGAAAT ACAACCATAT ATAAGCAGAT GTGCTGTATG TGAGGCCCCA 
GCCCAAGCAG TAGCAGTTCA CAGCCAAGAT CAGTCAATTC CTCCCTGCCC AATGAACTGG 
AGAAGTCTTT GGATAGGATA TTCTTTTCTG ATGCACACTG GATCTGGTGA TCAGGGAGGT 
GGGCAGTCCC TTATGTCACC AGGAAGCTGC TTAGAAGACT TCAGATCAGC ACCGTTCATT 
GAATGCCAGG GTCAGCGGGG AACTTGTCAG TTCTTTGCTA ATGAATACAG CTTTTGGCTG 
ACAACAGTGA TGCCTGACTT GCAGTTTGCA TCAGCCCCCT TGTCAGGAAC TCTTAAAGAA 
GGACAAGAGC AGAGGAAAAA AATCAGTAGA TGCCAGGTAT GCCTGAAGCA TGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_015276987.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015132473.1 Gallus gallus collagen, type IV, alpha 4 (COL4A4), transcript variant X2, mRNA.

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CloneID	OGa39728
Clone ID Related Accession (Same CDS sequence)	XM_015276986.1 , XM_015276987.1 , XM_015276987.2 , XM_015276986.2
Accession Version	XM_015276987.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	collagen alpha-4(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471743.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTTAA CAAAGGATTC ATTTGAACGA ATTAAGTGGC TTATCAGAAC TGCCTGGTGG 
CTGCTGATTG TTTTTTCAGC ACAAGAAATT GATGGGGGAG GATACGCATA TATTGACCCA 
TGTGGGGGAC AGGATTGTTC GGTTTGTCGG TGCTTTCCAG AAAAAGGATC AAGAGGTCAA 
CCTGGTGAGC TGGGAGCTCA AGGTCCAATT GGATCGTTAG GACCTACAGG ACCAGCTGGT 
CTGCCAGGGG AAAAAGGACA GAGAGGTGAG AATGGGCAGC CTGGGCCAGC TGGAGAAAAA 
GGTGATAAGG GCCCAACTGG AGTCCCAGGA TTTCCTGGTT TAGATGGTGT TCCTGGATTG 
CCAGGAAGTG AAGGACCCAG GGGCAAGCAC GGTGTAGATG GCTGCAATGG CTCAAGAGGA 
GATCCAGGAT TTCCAGGCGA AAATGGATAT ATTGGACCAA GAGGTCCTTA CGGAAACCCA 
GGGCAAAAAG GAGAAAAAGG AAATTCAGTG TATGTTTCGT ATTATGGAAA AGGACCCCCA 
GGAGACAGAG GTGATCCTGG CCCACCTGGT ATGCCCGGAC CTAGAGGATC GGCAGGTACA 
ACAGGCCCAC CTGGATACCC AGGCCAACCA GGCATGCCCG GTATTCCAGG TTACCCTGGT 
TTGCCAGGTG AACAGGGAAA TCCAGGTATT GGAGTGGATG GACAAAAGGG GGAGCCGGGT 
GATGTTGGAC TTCCCGGGCC ACCTGGGTCA CCACTGTTAG TTGGACCTCC TGGAGCTCAG 
CTGTTCAAAG GAGAAAAGGG CCAAAAAGGA CTACCTGGGA TAACGGGATA CAGAGGACCA 
CATGGACCTA AAGGAATACC TGGGAAAGGA GAAAAGGGTG AAAAAGGCAT TCCTGGATTC 
CCTGGATTGC GGGGTAGCCC TGGTTCTCAT GGACCTGCAG GCTTTCCTGG AATAAAGGGA 
GAAATGGGTG TTGCTGGTTT TCCTGGGAAA CCTGGCTATC CAGGCATCAA GGGGGATCCT 
GGAGAAAAAG GGCTCCCCGG TCCTCCTGGA ACTCCACTGC TTCCTATGAA AGGCCCACCA 
GGAGATCCTG GATTTCCAGG TCCTGCGGGT GACATGGGGT CAGTTGGACC TTCTGGCCCT 
GTGGGTATGC TAGGAAGACC AGGAGACGAT GGAACAAGTC TGCCTGGCCT GCCAGGAATA 
AGTGGGGCAC CAGGACCTCA AGGTTTTCAG GGTGACCCTG GTTTGCCTGG AACAGGTGAA 
GCAATTCCAG GAAGACCTGG ATTTCCTGGA CCACCTGGAC TACCAGGACA GCCAGGAAGA 
CAAGGCTTAC CTGGACTACC TAGTGTAATT TGTACTGAGA GAGGGATTCC TGGGGAACCT 
GGAGCAAAAG GTCAGATAGG CCTTCCGGGT AGAAAAGGTG AAAAAGGAGA AAAAGGAAAT 
CAAGGCCTCT GCTCATGTAG TGCTGGTCCT CCTGGTCCCC GTGGTATACA AGGACCTTCA 
GGTACTCAAG GGAAGAAAGG ACAAATGGGT TACCCTGGAA GACGTGGAGA AAAGGGTGAT 
TCAGGCTTAT CTGGTGCAGT GGGATCACCA GGACTCCCTG GGACACCTGG TTCTGGAGGA 
CAACATGGTG AAAAAGGAGA GAAGGGTGAC CAGGGAAGAG TTTCAATAAA AGGAATAAAA 
GGTGAAAGAG GTCCTACTGG GGTTCCAGGA TTTCCAGGCC AAAAAGGTAA TGATGGCAGA 
GATGGGGATT TAGGATTGCC TGGGGAAAAA GGAGATGAGG GTGATTCTGG AGTACCAATA 
CCAGGTGATA AAGGATTTCC TGGAGTTCCA GGACTCCCTG GAGTAAAAGG ACCAATGGGA 
TTGCCTGGTT TGGGGTTTCC TGGCCCTCCG GGAGTTAGAG GTACCCCTGG AGACTTTGGT 
GATGATGGTA ACATTGGTCT GCCTGGTCCA AAGGGTCAGA AAGGTGACAC TATCTGTATT 
ACATCACCGT ACCCTGGAAA CCCAGGTCCC CCAGGTTTTA AAGGTGTTCA AGGACCAAAG 
GGTTTAAAAG GACTCCCTGG TCTTCCTGGA CGAAGTGGCT TTGATGGGCC AAAAGGCCAG 
CAAGGCAGGC CAGGAGCAGG AATCCCTGGA CCAGAAGGCT TCCGAGGCAC AGCTGGTGAT 
CCTGGTGATG AAGGTGAAAG AGGATCTACC ATTGATGGTA AAAATGGTGC TCCAGGTCCA 
CCTGGCATAG ATGGCCAGAA GGGTGTTTCA GGTGATACTA CATATGGCCC TCCAGGATTC 
CCAGGCAACA GGGGACTGCC AGGACCACCT GGTGCACAAG GAGCAAGGGG TGATCCAGGC 
ATTCCTGGGC TTCAAGGGCA TCCTGGGACC CCAGGATTTC CAGGTGCTAA AGGTTTTAGA 
GGTCCAGAAG GTGATACAGG AGCGCCAGGC TGTCCAGGCA CCCCTGGTCC ACCATGTGAC 
ACACAATTAC CAGGGCCACC TGGACTCAGA GGTGTCATAG GCCTGCCAGG TTCTCAAGGC 
TTACCAGGCT TTAAAGGTCA GAAAGGAGAC AGGGGCCTAG CTGGGCCACC TGGAATCAAG 
GGTCTCAAGG GCTCTCCTGG CTCACAGGGC CCCCCAGGTC CTCCAGGGTC CCGAGGACTT 
CCAGGACTAC CAGGCAATAA AGGACAACCT GGCTTTCCAG GTCAAACAGG AAGCAAAGGG 
ATTCAAGGAC CCCAAGGATT CCCTGGACTC CCAGGAACAC AAGGCCAAAT GGGAATTCCT 
GGTGTAAAAG GGGAAGAAGG AAACATGGGT CCACCTGGGC CTAGTGGAGA ATGTGGGGAT 
ATGGGAATGA AAGGTGAAAG AGGGCTTCCA GGAGGTGGTG GCAGCATTAA CATTCGGCTT 
GAGAAAGGAC AAAAAGGAGA ACCAGGGTTT CCTGGTGAGA ATGGATTTAG AGGTGAAAGA 
GGTGAAAAAG GAAATACAGG TTTCAGAGGT GCCCCAGGAT TTCCAGGTAA CAACGGTGCC 
CCAGGACTTC CAGGTGACCA TGGTGACATT GGACTGATGG GATTTCCAGG ATCACGTGGT 
TTCCCAGGAC CAAAGGGCTC TAAGGGAGTT ACAGGTTTCA AAGGACTACC AGGTGACCAG 
GGTGATGTTG GCTCACCAGG AATGCCTGGA AACCCAGGAC TGACAGGCCC CAAAGGACCT 
AAAGGAAAGA GAGGTGACCC AACTCTTCTG CTTGGTACAC AAGGTCAGAA AGGGCCTCCT 
GGAGATCTAG GATTACCAGG ATTCTGTGGA TTCCCAGGTG AGAAAGGTTC ACCAGGTATC 
CGAGGGCAAC CTGGAAGACC AGGATCCAAG GGCGAGCCTG GATACCCAGG AATACCTGGA 
TTTCCAGGAG CTACAGGTCC TCCAGGATTG CCAGGAGAAC CTGGAGAAAA AGGGAAACAT 
GGAATTTTGG GACCTCCAGG GCTGCTAGGA TTAACTGGAT CACCAGGTAG AAAAGGTCTT 
CCTGGACTGC CTGGACTGGA TGGCTTGGAC GGACTAAAAG GTCAAAAAGG TTCTGCAGGA 
GCTCCAGGTC AAAGTGAAAC AGGGCGCCCA GGATACTCTG GAGAACCTGG ACCAAAAGGA 
GAGAGAGGTG AACCTGGATG GCCTGGTATT TCCATTCCAG GCACCCCTGG AGAAAGAGGA 
TTCCCAGGAT TCCCAGGTAG AAGAGGACCT GTTGGACCCA TTGGACCTAG GGGACCATCT 
CCCAGTAGTG CTCCTCCTGG TCTTCCAGGT GATCAAGGAC CACCTGGTTT AGATGGCATC 
AGAGGTCAGC CTGGGAATCC TGGTCCTCCT GGAGAGACTG TCTTTGTACG AGGAGATCCA 
GGTGACACCG GTATTCGAGG GGCACCAGGC ATTCCTGGAC ACCGAGGGCT ACAAGGAGCC 
AGAGGTCTGC CAGGAAATCA AGGAAGAGAA GGTCCAAAGG GTCCAATGGG AATCCATGGC 
TTACAGGGTC CACCTGGTGC TGTAGGACAA CCAGGAGACC AAGGTTTTCA AGGAAAACCT 
GGTCCTAAAG GTCCCACAGG GTTCATAGGT GAGCCTGGTG AACCAGGAAA AACAGATGAC 
TCATGTCCTG CAATCCCAGG GCCTCCAGGG GAAGCAGGAC ATAGAGGAGA TGATGGTTCT 
ATAGGATTAC CAGGGCCAAT AGGTCACCCT GGACCCCAAG GAAGGAAAGG TGAAGAAGGG 
TCATGTGGCC TGCCAGGTCA AGATGGCTTA CCAGGAGCAC CTGGACCACC AGGTGACCAA 
GGGCATGTGG GAGAACAAGG ATATGCTGGA CCACAAGGTC CACCTGGCCA GACTGGTATC 
CCAGGACCAC CAGGGCCCCA TGTTAGATCT TCAAGTGGAT TCTTGCTCGT CCTTCATAGT 
CAGTCAGACA GAGAACCACT TTGTCCACAG GGGATGCCAA AATTATGGAC TGGCTATAGT 
CTGCTGTACC TGGAAGGACA AGAGAAAGCT CATAATCAAG ATCTTGGTCT GGCAGGATCT 
TGTCTTCCTG TATTTAATAC CATGCCCTTT GCTTACTGTA ACATCAACCA AGTTTGTTAC 
TATGCCAGCC GAAATGATAA GTCCTACTGG TTGTCAAGTG CCGCTCCCTT ACCAATGACA 
CCACTGTCTG AGGAAGAAAT ACAACCATAT ATAAGCAGAT GTGCTGTATG TGAGGCCCCA 
GCCCAAGCAG TAGCAGTTCA CAGCCAAGAT CAGTCAATTC CTCCCTGCCC AATGAACTGG 
AGAAGTCTTT GGATAGGATA TTCTTTTCTG ATGCACACTG GATCTGGTGA TCAGGGAGGT 
GGGCAGTCCC TTATGTCACC AGGAAGCTGC TTAGAAGACT TCAGATCAGC ACCGTTCATT 
GAATGCCAGG GTCAGCGGGG AACTTGTCAG TTCTTTGCTA ATGAATACAG CTTTTGGCTG 
ACAACAGTGA TGCCTGACTT GCAGTTTGCA TCAGCCCCCT TGTCAGGAAC TCTTAAAGAA 
GGACAAGAGC AGAGGAAAAA AATCAGTAGA TGCCAGGTAT GCCTGAAGCA TGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015132473.1
CDS	298..5334
Translation	MALTKDSFERIKWLIRTAWWLLIVFSAQEIDGGGYAYIDPCGGQDCSVCRCFPEKGSRGQPGELGAQGPIGSLGPTGPAGLPGEKGQRGENGQPGPAGEKGDKGPTGVPGFPGLDGVPGLPGSEGPRGKHGVDGCNGSRGDPGFPGENGYIGPRGPYGNPGQKGEKGNSVYVSYYGKGPPGDRGDPGPPGMPGPRGSAGTTGPPGYPGQPGMPGIPGYPGLPGEQGNPGIGVDGQKGEPGDVGLPGPPGSPLLVGPPGAQLFKGEKGQKGLPGITGYRGPHGPKGIPGKGEKGEKGIPGFPGLRGSPGSHGPAGFPGIKGEMGVAGFPGKPGYPGIKGDPGEKGLPGPPGTPLLPMKGPPGDPGFPGPAGDMGSVGPSGPVGMLGRPGDDGTSLPGLPGISGAPGPQGFQGDPGLPGTGEAIPGRPGFPGPPGLPGQPGRQGLPGLPSVICTERGIPGEPGAKGQIGLPGRKGEKGEKGNQGLCSCSAGPPGPRGIQGPSGTQGKKGQMGYPGRRGEKGDSGLSGAVGSPGLPGTPGSGGQHGEKGEKGDQGRVSIKGIKGERGPTGVPGFPGQKGNDGRDGDLGLPGEKGDEGDSGVPIPGDKGFPGVPGLPGVKGPMGLPGLGFPGPPGVRGTPGDFGDDGNIGLPGPKGQKGDTICITSPYPGNPGPPGFKGVQGPKGLKGLPGLPGRSGFDGPKGQQGRPGAGIPGPEGFRGTAGDPGDEGERGSTIDGKNGAPGPPGIDGQKGVSGDTTYGPPGFPGNRGLPGPPGAQGARGDPGIPGLQGHPGTPGFPGAKGFRGPEGDTGAPGCPGTPGPPCDTQLPGPPGLRGVIGLPGSQGLPGFKGQKGDRGLAGPPGIKGLKGSPGSQGPPGPPGSRGLPGLPGNKGQPGFPGQTGSKGIQGPQGFPGLPGTQGQMGIPGVKGEEGNMGPPGPSGECGDMGMKGERGLPGGGGSINIRLEKGQKGEPGFPGENGFRGERGEKGNTGFRGAPGFPGNNGAPGLPGDHGDIGLMGFPGSRGFPGPKGSKGVTGFKGLPGDQGDVGSPGMPGNPGLTGPKGPKGKRGDPTLLLGTQGQKGPPGDLGLPGFCGFPGEKGSPGIRGQPGRPGSKGEPGYPGIPGFPGATGPPGLPGEPGEKGKHGILGPPGLLGLTGSPGRKGLPGLPGLDGLDGLKGQKGSAGAPGQSETGRPGYSGEPGPKGERGEPGWPGISIPGTPGERGFPGFPGRRGPVGPIGPRGPSPSSAPPGLPGDQGPPGLDGIRGQPGNPGPPGETVFVRGDPGDTGIRGAPGIPGHRGLQGARGLPGNQGREGPKGPMGIHGLQGPPGAVGQPGDQGFQGKPGPKGPTGFIGEPGEPGKTDDSCPAIPGPPGEAGHRGDDGSIGLPGPIGHPGPQGRKGEEGSCGLPGQDGLPGAPGPPGDQGHVGEQGYAGPQGPPGQTGIPGPPGPHVRSSSGFLLVLHSQSDREPLCPQGMPKLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFNTMPFAYCNINQVCYYASRNDKSYWLSSAAPLPMTPLSEEEIQPYISRCAVCEAPAQAVAVHSQDQSIPPCPMNWRSLWIGYSFLMHTGSGDQGGGQSLMSPGSCLEDFRSAPFIECQGQRGTCQFFANEYSFWLTTVMPDLQFASAPLSGTLKEGQEQRKKISRCQVCLKHD

Target ORF information:

RefSeq Version	XM_015276987.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus collagen, type IV, alpha 4 (COL4A4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276987.1

ORF Insert Sequence:

ATGGCGTTAA CAAAGGATTC ATTTGAACGA ATTAAGTGGC TTATCAGAAC TGCCTGGTGG 
CTGCTGATTG TTTTTTCAGC ACAAGAAATT GATGGGGGAG GATACGCATA TATTGACCCA 
TGTGGGGGAC AGGATTGTTC GGTTTGTCGG TGCTTTCCAG AAAAAGGATC AAGAGGTCAA 
CCTGGTGAGC TGGGAGCTCA AGGTCCAATT GGATCGTTAG GACCTACAGG ACCAGCTGGT 
CTGCCAGGGG AAAAAGGACA GAGAGGTGAG AATGGGCAGC CTGGGCCAGC TGGAGAAAAA 
GGTGATAAGG GCCCAACTGG AGTCCCAGGA TTTCCTGGTT TAGATGGTGT TCCTGGATTG 
CCAGGAAGTG AAGGACCCAG GGGCAAGCAC GGTGTAGATG GCTGCAATGG CTCAAGAGGA 
GATCCAGGAT TTCCAGGCGA AAATGGATAT ATTGGACCAA GAGGTCCTTA CGGAAACCCA 
GGGCAAAAAG GAGAAAAAGG AAATTCAGTG TATGTTTCGT ATTATGGAAA AGGACCCCCA 
GGAGACAGAG GTGATCCTGG CCCACCTGGT ATGCCCGGAC CTAGAGGATC GGCAGGTACA 
ACAGGCCCAC CTGGATACCC AGGCCAACCA GGCATGCCCG GTATTCCAGG TTACCCTGGT 
TTGCCAGGTG AACAGGGAAA TCCAGGTATT GGAGTGGATG GACAAAAGGG GGAGCCGGGT 
GATGTTGGAC TTCCCGGGCC ACCTGGGTCA CCACTGTTAG TTGGACCTCC TGGAGCTCAG 
CTGTTCAAAG GAGAAAAGGG CCAAAAAGGA CTACCTGGGA TAACGGGATA CAGAGGACCA 
CATGGACCTA AAGGAATACC TGGGAAAGGA GAAAAGGGTG AAAAAGGCAT TCCTGGATTC 
CCTGGATTGC GGGGTAGCCC TGGTTCTCAT GGACCTGCAG GCTTTCCTGG AATAAAGGGA 
GAAATGGGTG TTGCTGGTTT TCCTGGGAAA CCTGGCTATC CAGGCATCAA GGGGGATCCT 
GGAGAAAAAG GGCTCCCCGG TCCTCCTGGA ACTCCACTGC TTCCTATGAA AGGCCCACCA 
GGAGATCCTG GATTTCCAGG TCCTGCGGGT GACATGGGGT CAGTTGGACC TTCTGGCCCT 
GTGGGTATGC TAGGAAGACC AGGAGACGAT GGAACAAGTC TGCCTGGCCT GCCAGGAATA 
AGTGGGGCAC CAGGACCTCA AGGTTTTCAG GGTGACCCTG GTTTGCCTGG AACAGGTGAA 
GCAATTCCAG GAAGACCTGG ATTTCCTGGA CCACCTGGAC TACCAGGACA GCCAGGAAGA 
CAAGGCTTAC CTGGACTACC TAGTGTAATT TGTACTGAGA GAGGGATTCC TGGGGAACCT 
GGAGCAAAAG GTCAGATAGG CCTTCCGGGT AGAAAAGGTG AAAAAGGAGA AAAAGGAAAT 
CAAGGCCTCT GCTCATGTAG TGCTGGTCCT CCTGGTCCCC GTGGTATACA AGGACCTTCA 
GGTACTCAAG GGAAGAAAGG ACAAATGGGT TACCCTGGAA GACGTGGAGA AAAGGGTGAT 
TCAGGCTTAT CTGGTGCAGT GGGATCACCA GGACTCCCTG GGACACCTGG TTCTGGAGGA 
CAACATGGTG AAAAAGGAGA GAAGGGTGAC CAGGGAAGAG TTTCAATAAA AGGAATAAAA 
GGTGAAAGAG GTCCTACTGG GGTTCCAGGA TTTCCAGGCC AAAAAGGTAA TGATGGCAGA 
GATGGGGATT TAGGATTGCC TGGGGAAAAA GGAGATGAGG GTGATTCTGG AGTACCAATA 
CCAGGTGATA AAGGATTTCC TGGAGTTCCA GGACTCCCTG GAGTAAAAGG ACCAATGGGA 
TTGCCTGGTT TGGGGTTTCC TGGCCCTCCG GGAGTTAGAG GTACCCCTGG AGACTTTGGT 
GATGATGGTA ACATTGGTCT GCCTGGTCCA AAGGGTCAGA AAGGTGACAC TATCTGTATT 
ACATCACCGT ACCCTGGAAA CCCAGGTCCC CCAGGTTTTA AAGGTGTTCA AGGACCAAAG 
GGTTTAAAAG GACTCCCTGG TCTTCCTGGA CGAAGTGGCT TTGATGGGCC AAAAGGCCAG 
CAAGGCAGGC CAGGAGCAGG AATCCCTGGA CCAGAAGGCT TCCGAGGCAC AGCTGGTGAT 
CCTGGTGATG AAGGTGAAAG AGGATCTACC ATTGATGGTA AAAATGGTGC TCCAGGTCCA 
CCTGGCATAG ATGGCCAGAA GGGTGTTTCA GGTGATACTA CATATGGCCC TCCAGGATTC 
CCAGGCAACA GGGGACTGCC AGGACCACCT GGTGCACAAG GAGCAAGGGG TGATCCAGGC 
ATTCCTGGGC TTCAAGGGCA TCCTGGGACC CCAGGATTTC CAGGTGCTAA AGGTTTTAGA 
GGTCCAGAAG GTGATACAGG AGCGCCAGGC TGTCCAGGCA CCCCTGGTCC ACCATGTGAC 
ACACAATTAC CAGGGCCACC TGGACTCAGA GGTGTCATAG GCCTGCCAGG TTCTCAAGGC 
TTACCAGGCT TTAAAGGTCA GAAAGGAGAC AGGGGCCTAG CTGGGCCACC TGGAATCAAG 
GGTCTCAAGG GCTCTCCTGG CTCACAGGGC CCCCCAGGTC CTCCAGGGTC CCGAGGACTT 
CCAGGACTAC CAGGCAATAA AGGACAACCT GGCTTTCCAG GTCAAACAGG AAGCAAAGGG 
ATTCAAGGAC CCCAAGGATT CCCTGGACTC CCAGGAACAC AAGGCCAAAT GGGAATTCCT 
GGTGTAAAAG GGGAAGAAGG AAACATGGGT CCACCTGGGC CTAGTGGAGA ATGTGGGGAT 
ATGGGAATGA AAGGTGAAAG AGGGCTTCCA GGAGGTGGTG GCAGCATTAA CATTCGGCTT 
GAGAAAGGAC AAAAAGGAGA ACCAGGGTTT CCTGGTGAGA ATGGATTTAG AGGTGAAAGA 
GGTGAAAAAG GAAATACAGG TTTCAGAGGT GCCCCAGGAT TTCCAGGTAA CAACGGTGCC 
CCAGGACTTC CAGGTGACCA TGGTGACATT GGACTGATGG GATTTCCAGG ATCACGTGGT 
TTCCCAGGAC CAAAGGGCTC TAAGGGAGTT ACAGGTTTCA AAGGACTACC AGGTGACCAG 
GGTGATGTTG GCTCACCAGG AATGCCTGGA AACCCAGGAC TGACAGGCCC CAAAGGACCT 
AAAGGAAAGA GAGGTGACCC AACTCTTCTG CTTGGTACAC AAGGTCAGAA AGGGCCTCCT 
GGAGATCTAG GATTACCAGG ATTCTGTGGA TTCCCAGGTG AGAAAGGTTC ACCAGGTATC 
CGAGGGCAAC CTGGAAGACC AGGATCCAAG GGCGAGCCTG GATACCCAGG AATACCTGGA 
TTTCCAGGAG CTACAGGTCC TCCAGGATTG CCAGGAGAAC CTGGAGAAAA AGGGAAACAT 
GGAATTTTGG GACCTCCAGG GCTGCTAGGA TTAACTGGAT CACCAGGTAG AAAAGGTCTT 
CCTGGACTGC CTGGACTGGA TGGCTTGGAC GGACTAAAAG GTCAAAAAGG TTCTGCAGGA 
GCTCCAGGTC AAAGTGAAAC AGGGCGCCCA GGATACTCTG GAGAACCTGG ACCAAAAGGA 
GAGAGAGGTG AACCTGGATG GCCTGGTATT TCCATTCCAG GCACCCCTGG AGAAAGAGGA 
TTCCCAGGAT TCCCAGGTAG AAGAGGACCT GTTGGACCCA TTGGACCTAG GGGACCATCT 
CCCAGTAGTG CTCCTCCTGG TCTTCCAGGT GATCAAGGAC CACCTGGTTT AGATGGCATC 
AGAGGTCAGC CTGGGAATCC TGGTCCTCCT GGAGAGACTG TCTTTGTACG AGGAGATCCA 
GGTGACACCG GTATTCGAGG GGCACCAGGC ATTCCTGGAC ACCGAGGGCT ACAAGGAGCC 
AGAGGTCTGC CAGGAAATCA AGGAAGAGAA GGTCCAAAGG GTCCAATGGG AATCCATGGC 
TTACAGGGTC CACCTGGTGC TGTAGGACAA CCAGGAGACC AAGGTTTTCA AGGAAAACCT 
GGTCCTAAAG GTCCCACAGG GTTCATAGGT GAGCCTGGTG AACCAGGAAA AACAGATGAC 
TCATGTCCTG CAATCCCAGG GCCTCCAGGG GAAGCAGGAC ATAGAGGAGA TGATGGTTCT 
ATAGGATTAC CAGGGCCAAT AGGTCACCCT GGACCCCAAG GAAGGAAAGG TGAAGAAGGG 
TCATGTGGCC TGCCAGGTCA AGATGGCTTA CCAGGAGCAC CTGGACCACC AGGTGACCAA 
GGGCATGTGG GAGAACAAGG ATATGCTGGA CCACAAGGTC CACCTGGCCA GACTGGTATC 
CCAGGACCAC CAGGGCCCCA TGTTAGATCT TCAAGTGGAT TCTTGCTCGT CCTTCATAGT 
CAGTCAGACA GAGAACCACT TTGTCCACAG GGGATGCCAA AATTATGGAC TGGCTATAGT 
CTGCTGTACC TGGAAGGACA AGAGAAAGCT CATAATCAAG ATCTTGGTCT GGCAGGATCT 
TGTCTTCCTG TATTTAATAC CATGCCCTTT GCTTACTGTA ACATCAACCA AGTTTGTTAC 
TATGCCAGCC GAAATGATAA GTCCTACTGG TTGTCAAGTG CCGCTCCCTT ACCAATGACA 
CCACTGTCTG AGGAAGAAAT ACAACCATAT ATAAGCAGAT GTGCTGTATG TGAGGCCCCA 
GCCCAAGCAG TAGCAGTTCA CAGCCAAGAT CAGTCAATTC CTCCCTGCCC AATGAACTGG 
AGAAGTCTTT GGATAGGATA TTCTTTTCTG ATGCACACTG GATCTGGTGA TCAGGGAGGT 
GGGCAGTCCC TTATGTCACC AGGAAGCTGC TTAGAAGACT TCAGATCAGC ACCGTTCATT 
GAATGCCAGG GTCAGCGGGG AACTTGTCAG TTCTTTGCTA ATGAATACAG CTTTTGGCTG 
ACAACAGTGA TGCCTGACTT GCAGTTTGCA TCAGCCCCCT TGTCAGGAAC TCTTAAAGAA 
GGACAAGAGC AGAGGAAAAA AATCAGTAGA TGCCAGGTAT GCCTGAAGCA TGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_015276987.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015132473.1 Gallus gallus collagen type IV alpha 4 chain (COL4A4), transcript variant X2, mRNA.

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CloneID	OGa39728
Clone ID Related Accession (Same CDS sequence)	XM_015276986.1 , XM_015276987.1 , XM_015276987.2 , XM_015276986.2
Accession Version	XM_015276987.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	collagen alpha-4(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015276987.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTTAA CAAAGGATTC ATTTGAACGA ATTAAGTGGC TTATCAGAAC TGCCTGGTGG 
CTGCTGATTG TTTTTTCAGC ACAAGAAATT GATGGGGGAG GATACGCATA TATTGACCCA 
TGTGGGGGAC AGGATTGTTC GGTTTGTCGG TGCTTTCCAG AAAAAGGATC AAGAGGTCAA 
CCTGGTGAGC TGGGAGCTCA AGGTCCAATT GGATCGTTAG GACCTACAGG ACCAGCTGGT 
CTGCCAGGGG AAAAAGGACA GAGAGGTGAG AATGGGCAGC CTGGGCCAGC TGGAGAAAAA 
GGTGATAAGG GCCCAACTGG AGTCCCAGGA TTTCCTGGTT TAGATGGTGT TCCTGGATTG 
CCAGGAAGTG AAGGACCCAG GGGCAAGCAC GGTGTAGATG GCTGCAATGG CTCAAGAGGA 
GATCCAGGAT TTCCAGGCGA AAATGGATAT ATTGGACCAA GAGGTCCTTA CGGAAACCCA 
GGGCAAAAAG GAGAAAAAGG AAATTCAGTG TATGTTTCGT ATTATGGAAA AGGACCCCCA 
GGAGACAGAG GTGATCCTGG CCCACCTGGT ATGCCCGGAC CTAGAGGATC GGCAGGTACA 
ACAGGCCCAC CTGGATACCC AGGCCAACCA GGCATGCCCG GTATTCCAGG TTACCCTGGT 
TTGCCAGGTG AACAGGGAAA TCCAGGTATT GGAGTGGATG GACAAAAGGG GGAGCCGGGT 
GATGTTGGAC TTCCCGGGCC ACCTGGGTCA CCACTGTTAG TTGGACCTCC TGGAGCTCAG 
CTGTTCAAAG GAGAAAAGGG CCAAAAAGGA CTACCTGGGA TAACGGGATA CAGAGGACCA 
CATGGACCTA AAGGAATACC TGGGAAAGGA GAAAAGGGTG AAAAAGGCAT TCCTGGATTC 
CCTGGATTGC GGGGTAGCCC TGGTTCTCAT GGACCTGCAG GCTTTCCTGG AATAAAGGGA 
GAAATGGGTG TTGCTGGTTT TCCTGGGAAA CCTGGCTATC CAGGCATCAA GGGGGATCCT 
GGAGAAAAAG GGCTCCCCGG TCCTCCTGGA ACTCCACTGC TTCCTATGAA AGGCCCACCA 
GGAGATCCTG GATTTCCAGG TCCTGCGGGT GACATGGGGT CAGTTGGACC TTCTGGCCCT 
GTGGGTATGC TAGGAAGACC AGGAGACGAT GGAACAAGTC TGCCTGGCCT GCCAGGAATA 
AGTGGGGCAC CAGGACCTCA AGGTTTTCAG GGTGACCCTG GTTTGCCTGG AACAGGTGAA 
GCAATTCCAG GAAGACCTGG ATTTCCTGGA CCACCTGGAC TACCAGGACA GCCAGGAAGA 
CAAGGCTTAC CTGGACTACC TAGTGTAATT TGTACTGAGA GAGGGATTCC TGGGGAACCT 
GGAGCAAAAG GTCAGATAGG CCTTCCGGGT AGAAAAGGTG AAAAAGGAGA AAAAGGAAAT 
CAAGGCCTCT GCTCATGTAG TGCTGGTCCT CCTGGTCCCC GTGGTATACA AGGACCTTCA 
GGTACTCAAG GGAAGAAAGG ACAAATGGGT TACCCTGGAA GACGTGGAGA AAAGGGTGAT 
TCAGGCTTAT CTGGTGCAGT GGGATCACCA GGACTCCCTG GGACACCTGG TTCTGGAGGA 
CAACATGGTG AAAAAGGAGA GAAGGGTGAC CAGGGAAGAG TTTCAATAAA AGGAATAAAA 
GGTGAAAGAG GTCCTACTGG GGTTCCAGGA TTTCCAGGCC AAAAAGGTAA TGATGGCAGA 
GATGGGGATT TAGGATTGCC TGGGGAAAAA GGAGATGAGG GTGATTCTGG AGTACCAATA 
CCAGGTGATA AAGGATTTCC TGGAGTTCCA GGACTCCCTG GAGTAAAAGG ACCAATGGGA 
TTGCCTGGTT TGGGGTTTCC TGGCCCTCCG GGAGTTAGAG GTACCCCTGG AGACTTTGGT 
GATGATGGTA ACATTGGTCT GCCTGGTCCA AAGGGTCAGA AAGGTGACAC TATCTGTATT 
ACATCACCGT ACCCTGGAAA CCCAGGTCCC CCAGGTTTTA AAGGTGTTCA AGGACCAAAG 
GGTTTAAAAG GACTCCCTGG TCTTCCTGGA CGAAGTGGCT TTGATGGGCC AAAAGGCCAG 
CAAGGCAGGC CAGGAGCAGG AATCCCTGGA CCAGAAGGCT TCCGAGGCAC AGCTGGTGAT 
CCTGGTGATG AAGGTGAAAG AGGATCTACC ATTGATGGTA AAAATGGTGC TCCAGGTCCA 
CCTGGCATAG ATGGCCAGAA GGGTGTTTCA GGTGATACTA CATATGGCCC TCCAGGATTC 
CCAGGCAACA GGGGACTGCC AGGACCACCT GGTGCACAAG GAGCAAGGGG TGATCCAGGC 
ATTCCTGGGC TTCAAGGGCA TCCTGGGACC CCAGGATTTC CAGGTGCTAA AGGTTTTAGA 
GGTCCAGAAG GTGATACAGG AGCGCCAGGC TGTCCAGGCA CCCCTGGTCC ACCATGTGAC 
ACACAATTAC CAGGGCCACC TGGACTCAGA GGTGTCATAG GCCTGCCAGG TTCTCAAGGC 
TTACCAGGCT TTAAAGGTCA GAAAGGAGAC AGGGGCCTAG CTGGGCCACC TGGAATCAAG 
GGTCTCAAGG GCTCTCCTGG CTCACAGGGC CCCCCAGGTC CTCCAGGGTC CCGAGGACTT 
CCAGGACTAC CAGGCAATAA AGGACAACCT GGCTTTCCAG GTCAAACAGG AAGCAAAGGG 
ATTCAAGGAC CCCAAGGATT CCCTGGACTC CCAGGAACAC AAGGCCAAAT GGGAATTCCT 
GGTGTAAAAG GGGAAGAAGG AAACATGGGT CCACCTGGGC CTAGTGGAGA ATGTGGGGAT 
ATGGGAATGA AAGGTGAAAG AGGGCTTCCA GGAGGTGGTG GCAGCATTAA CATTCGGCTT 
GAGAAAGGAC AAAAAGGAGA ACCAGGGTTT CCTGGTGAGA ATGGATTTAG AGGTGAAAGA 
GGTGAAAAAG GAAATACAGG TTTCAGAGGT GCCCCAGGAT TTCCAGGTAA CAACGGTGCC 
CCAGGACTTC CAGGTGACCA TGGTGACATT GGACTGATGG GATTTCCAGG ATCACGTGGT 
TTCCCAGGAC CAAAGGGCTC TAAGGGAGTT ACAGGTTTCA AAGGACTACC AGGTGACCAG 
GGTGATGTTG GCTCACCAGG AATGCCTGGA AACCCAGGAC TGACAGGCCC CAAAGGACCT 
AAAGGAAAGA GAGGTGACCC AACTCTTCTG CTTGGTACAC AAGGTCAGAA AGGGCCTCCT 
GGAGATCTAG GATTACCAGG ATTCTGTGGA TTCCCAGGTG AGAAAGGTTC ACCAGGTATC 
CGAGGGCAAC CTGGAAGACC AGGATCCAAG GGCGAGCCTG GATACCCAGG AATACCTGGA 
TTTCCAGGAG CTACAGGTCC TCCAGGATTG CCAGGAGAAC CTGGAGAAAA AGGGAAACAT 
GGAATTTTGG GACCTCCAGG GCTGCTAGGA TTAACTGGAT CACCAGGTAG AAAAGGTCTT 
CCTGGACTGC CTGGACTGGA TGGCTTGGAC GGACTAAAAG GTCAAAAAGG TTCTGCAGGA 
GCTCCAGGTC AAAGTGAAAC AGGGCGCCCA GGATACTCTG GAGAACCTGG ACCAAAAGGA 
GAGAGAGGTG AACCTGGATG GCCTGGTATT TCCATTCCAG GCACCCCTGG AGAAAGAGGA 
TTCCCAGGAT TCCCAGGTAG AAGAGGACCT GTTGGACCCA TTGGACCTAG GGGACCATCT 
CCCAGTAGTG CTCCTCCTGG TCTTCCAGGT GATCAAGGAC CACCTGGTTT AGATGGCATC 
AGAGGTCAGC CTGGGAATCC TGGTCCTCCT GGAGAGACTG TCTTTGTACG AGGAGATCCA 
GGTGACACCG GTATTCGAGG GGCACCAGGC ATTCCTGGAC ACCGAGGGCT ACAAGGAGCC 
AGAGGTCTGC CAGGAAATCA AGGAAGAGAA GGTCCAAAGG GTCCAATGGG AATCCATGGC 
TTACAGGGTC CACCTGGTGC TGTAGGACAA CCAGGAGACC AAGGTTTTCA AGGAAAACCT 
GGTCCTAAAG GTCCCACAGG GTTCATAGGT GAGCCTGGTG AACCAGGAAA AACAGATGAC 
TCATGTCCTG CAATCCCAGG GCCTCCAGGG GAAGCAGGAC ATAGAGGAGA TGATGGTTCT 
ATAGGATTAC CAGGGCCAAT AGGTCACCCT GGACCCCAAG GAAGGAAAGG TGAAGAAGGG 
TCATGTGGCC TGCCAGGTCA AGATGGCTTA CCAGGAGCAC CTGGACCACC AGGTGACCAA 
GGGCATGTGG GAGAACAAGG ATATGCTGGA CCACAAGGTC CACCTGGCCA GACTGGTATC 
CCAGGACCAC CAGGGCCCCA TGTTAGATCT TCAAGTGGAT TCTTGCTCGT CCTTCATAGT 
CAGTCAGACA GAGAACCACT TTGTCCACAG GGGATGCCAA AATTATGGAC TGGCTATAGT 
CTGCTGTACC TGGAAGGACA AGAGAAAGCT CATAATCAAG ATCTTGGTCT GGCAGGATCT 
TGTCTTCCTG TATTTAATAC CATGCCCTTT GCTTACTGTA ACATCAACCA AGTTTGTTAC 
TATGCCAGCC GAAATGATAA GTCCTACTGG TTGTCAAGTG CCGCTCCCTT ACCAATGACA 
CCACTGTCTG AGGAAGAAAT ACAACCATAT ATAAGCAGAT GTGCTGTATG TGAGGCCCCA 
GCCCAAGCAG TAGCAGTTCA CAGCCAAGAT CAGTCAATTC CTCCCTGCCC AATGAACTGG 
AGAAGTCTTT GGATAGGATA TTCTTTTCTG ATGCACACTG GATCTGGTGA TCAGGGAGGT 
GGGCAGTCCC TTATGTCACC AGGAAGCTGC TTAGAAGACT TCAGATCAGC ACCGTTCATT 
GAATGCCAGG GTCAGCGGGG AACTTGTCAG TTCTTTGCTA ATGAATACAG CTTTTGGCTG 
ACAACAGTGA TGCCTGACTT GCAGTTTGCA TCAGCCCCCT TGTCAGGAAC TCTTAAAGAA 
GGACAAGAGC AGAGGAAAAA AATCAGTAGA TGCCAGGTAT GCCTGAAGCA TGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015132473.1
CDS	280..5316
Translation	MALTKDSFERIKWLIRTAWWLLIVFSAQEIDGGGYAYIDPCGGQDCSVCRCFPEKGSRGQPGELGAQGPIGSLGPTGPAGLPGEKGQRGENGQPGPAGEKGDKGPTGVPGFPGLDGVPGLPGSEGPRGKHGVDGCNGSRGDPGFPGENGYIGPRGPYGNPGQKGEKGNSVYVSYYGKGPPGDRGDPGPPGMPGPRGSAGTTGPPGYPGQPGMPGIPGYPGLPGEQGNPGIGVDGQKGEPGDVGLPGPPGSPLLVGPPGAQLFKGEKGQKGLPGITGYRGPHGPKGIPGKGEKGEKGIPGFPGLRGSPGSHGPAGFPGIKGEMGVAGFPGKPGYPGIKGDPGEKGLPGPPGTPLLPMKGPPGDPGFPGPAGDMGSVGPSGPVGMLGRPGDDGTSLPGLPGISGAPGPQGFQGDPGLPGTGEAIPGRPGFPGPPGLPGQPGRQGLPGLPSVICTERGIPGEPGAKGQIGLPGRKGEKGEKGNQGLCSCSAGPPGPRGIQGPSGTQGKKGQMGYPGRRGEKGDSGLSGAVGSPGLPGTPGSGGQHGEKGEKGDQGRVSIKGIKGERGPTGVPGFPGQKGNDGRDGDLGLPGEKGDEGDSGVPIPGDKGFPGVPGLPGVKGPMGLPGLGFPGPPGVRGTPGDFGDDGNIGLPGPKGQKGDTICITSPYPGNPGPPGFKGVQGPKGLKGLPGLPGRSGFDGPKGQQGRPGAGIPGPEGFRGTAGDPGDEGERGSTIDGKNGAPGPPGIDGQKGVSGDTTYGPPGFPGNRGLPGPPGAQGARGDPGIPGLQGHPGTPGFPGAKGFRGPEGDTGAPGCPGTPGPPCDTQLPGPPGLRGVIGLPGSQGLPGFKGQKGDRGLAGPPGIKGLKGSPGSQGPPGPPGSRGLPGLPGNKGQPGFPGQTGSKGIQGPQGFPGLPGTQGQMGIPGVKGEEGNMGPPGPSGECGDMGMKGERGLPGGGGSINIRLEKGQKGEPGFPGENGFRGERGEKGNTGFRGAPGFPGNNGAPGLPGDHGDIGLMGFPGSRGFPGPKGSKGVTGFKGLPGDQGDVGSPGMPGNPGLTGPKGPKGKRGDPTLLLGTQGQKGPPGDLGLPGFCGFPGEKGSPGIRGQPGRPGSKGEPGYPGIPGFPGATGPPGLPGEPGEKGKHGILGPPGLLGLTGSPGRKGLPGLPGLDGLDGLKGQKGSAGAPGQSETGRPGYSGEPGPKGERGEPGWPGISIPGTPGERGFPGFPGRRGPVGPIGPRGPSPSSAPPGLPGDQGPPGLDGIRGQPGNPGPPGETVFVRGDPGDTGIRGAPGIPGHRGLQGARGLPGNQGREGPKGPMGIHGLQGPPGAVGQPGDQGFQGKPGPKGPTGFIGEPGEPGKTDDSCPAIPGPPGEAGHRGDDGSIGLPGPIGHPGPQGRKGEEGSCGLPGQDGLPGAPGPPGDQGHVGEQGYAGPQGPPGQTGIPGPPGPHVRSSSGFLLVLHSQSDREPLCPQGMPKLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFNTMPFAYCNINQVCYYASRNDKSYWLSSAAPLPMTPLSEEEIQPYISRCAVCEAPAQAVAVHSQDQSIPPCPMNWRSLWIGYSFLMHTGSGDQGGGQSLMSPGSCLEDFRSAPFIECQGQRGTCQFFANEYSFWLTTVMPDLQFASAPLSGTLKEGQEQRKKISRCQVCLKHD

Target ORF information:

RefSeq Version	XM_015276987.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus collagen type IV alpha 4 chain (COL4A4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276987.2

ORF Insert Sequence:

ATGGCGTTAA CAAAGGATTC ATTTGAACGA ATTAAGTGGC TTATCAGAAC TGCCTGGTGG 
CTGCTGATTG TTTTTTCAGC ACAAGAAATT GATGGGGGAG GATACGCATA TATTGACCCA 
TGTGGGGGAC AGGATTGTTC GGTTTGTCGG TGCTTTCCAG AAAAAGGATC AAGAGGTCAA 
CCTGGTGAGC TGGGAGCTCA AGGTCCAATT GGATCGTTAG GACCTACAGG ACCAGCTGGT 
CTGCCAGGGG AAAAAGGACA GAGAGGTGAG AATGGGCAGC CTGGGCCAGC TGGAGAAAAA 
GGTGATAAGG GCCCAACTGG AGTCCCAGGA TTTCCTGGTT TAGATGGTGT TCCTGGATTG 
CCAGGAAGTG AAGGACCCAG GGGCAAGCAC GGTGTAGATG GCTGCAATGG CTCAAGAGGA 
GATCCAGGAT TTCCAGGCGA AAATGGATAT ATTGGACCAA GAGGTCCTTA CGGAAACCCA 
GGGCAAAAAG GAGAAAAAGG AAATTCAGTG TATGTTTCGT ATTATGGAAA AGGACCCCCA 
GGAGACAGAG GTGATCCTGG CCCACCTGGT ATGCCCGGAC CTAGAGGATC GGCAGGTACA 
ACAGGCCCAC CTGGATACCC AGGCCAACCA GGCATGCCCG GTATTCCAGG TTACCCTGGT 
TTGCCAGGTG AACAGGGAAA TCCAGGTATT GGAGTGGATG GACAAAAGGG GGAGCCGGGT 
GATGTTGGAC TTCCCGGGCC ACCTGGGTCA CCACTGTTAG TTGGACCTCC TGGAGCTCAG 
CTGTTCAAAG GAGAAAAGGG CCAAAAAGGA CTACCTGGGA TAACGGGATA CAGAGGACCA 
CATGGACCTA AAGGAATACC TGGGAAAGGA GAAAAGGGTG AAAAAGGCAT TCCTGGATTC 
CCTGGATTGC GGGGTAGCCC TGGTTCTCAT GGACCTGCAG GCTTTCCTGG AATAAAGGGA 
GAAATGGGTG TTGCTGGTTT TCCTGGGAAA CCTGGCTATC CAGGCATCAA GGGGGATCCT 
GGAGAAAAAG GGCTCCCCGG TCCTCCTGGA ACTCCACTGC TTCCTATGAA AGGCCCACCA 
GGAGATCCTG GATTTCCAGG TCCTGCGGGT GACATGGGGT CAGTTGGACC TTCTGGCCCT 
GTGGGTATGC TAGGAAGACC AGGAGACGAT GGAACAAGTC TGCCTGGCCT GCCAGGAATA 
AGTGGGGCAC CAGGACCTCA AGGTTTTCAG GGTGACCCTG GTTTGCCTGG AACAGGTGAA 
GCAATTCCAG GAAGACCTGG ATTTCCTGGA CCACCTGGAC TACCAGGACA GCCAGGAAGA 
CAAGGCTTAC CTGGACTACC TAGTGTAATT TGTACTGAGA GAGGGATTCC TGGGGAACCT 
GGAGCAAAAG GTCAGATAGG CCTTCCGGGT AGAAAAGGTG AAAAAGGAGA AAAAGGAAAT 
CAAGGCCTCT GCTCATGTAG TGCTGGTCCT CCTGGTCCCC GTGGTATACA AGGACCTTCA 
GGTACTCAAG GGAAGAAAGG ACAAATGGGT TACCCTGGAA GACGTGGAGA AAAGGGTGAT 
TCAGGCTTAT CTGGTGCAGT GGGATCACCA GGACTCCCTG GGACACCTGG TTCTGGAGGA 
CAACATGGTG AAAAAGGAGA GAAGGGTGAC CAGGGAAGAG TTTCAATAAA AGGAATAAAA 
GGTGAAAGAG GTCCTACTGG GGTTCCAGGA TTTCCAGGCC AAAAAGGTAA TGATGGCAGA 
GATGGGGATT TAGGATTGCC TGGGGAAAAA GGAGATGAGG GTGATTCTGG AGTACCAATA 
CCAGGTGATA AAGGATTTCC TGGAGTTCCA GGACTCCCTG GAGTAAAAGG ACCAATGGGA 
TTGCCTGGTT TGGGGTTTCC TGGCCCTCCG GGAGTTAGAG GTACCCCTGG AGACTTTGGT 
GATGATGGTA ACATTGGTCT GCCTGGTCCA AAGGGTCAGA AAGGTGACAC TATCTGTATT 
ACATCACCGT ACCCTGGAAA CCCAGGTCCC CCAGGTTTTA AAGGTGTTCA AGGACCAAAG 
GGTTTAAAAG GACTCCCTGG TCTTCCTGGA CGAAGTGGCT TTGATGGGCC AAAAGGCCAG 
CAAGGCAGGC CAGGAGCAGG AATCCCTGGA CCAGAAGGCT TCCGAGGCAC AGCTGGTGAT 
CCTGGTGATG AAGGTGAAAG AGGATCTACC ATTGATGGTA AAAATGGTGC TCCAGGTCCA 
CCTGGCATAG ATGGCCAGAA GGGTGTTTCA GGTGATACTA CATATGGCCC TCCAGGATTC 
CCAGGCAACA GGGGACTGCC AGGACCACCT GGTGCACAAG GAGCAAGGGG TGATCCAGGC 
ATTCCTGGGC TTCAAGGGCA TCCTGGGACC CCAGGATTTC CAGGTGCTAA AGGTTTTAGA 
GGTCCAGAAG GTGATACAGG AGCGCCAGGC TGTCCAGGCA CCCCTGGTCC ACCATGTGAC 
ACACAATTAC CAGGGCCACC TGGACTCAGA GGTGTCATAG GCCTGCCAGG TTCTCAAGGC 
TTACCAGGCT TTAAAGGTCA GAAAGGAGAC AGGGGCCTAG CTGGGCCACC TGGAATCAAG 
GGTCTCAAGG GCTCTCCTGG CTCACAGGGC CCCCCAGGTC CTCCAGGGTC CCGAGGACTT 
CCAGGACTAC CAGGCAATAA AGGACAACCT GGCTTTCCAG GTCAAACAGG AAGCAAAGGG 
ATTCAAGGAC CCCAAGGATT CCCTGGACTC CCAGGAACAC AAGGCCAAAT GGGAATTCCT 
GGTGTAAAAG GGGAAGAAGG AAACATGGGT CCACCTGGGC CTAGTGGAGA ATGTGGGGAT 
ATGGGAATGA AAGGTGAAAG AGGGCTTCCA GGAGGTGGTG GCAGCATTAA CATTCGGCTT 
GAGAAAGGAC AAAAAGGAGA ACCAGGGTTT CCTGGTGAGA ATGGATTTAG AGGTGAAAGA 
GGTGAAAAAG GAAATACAGG TTTCAGAGGT GCCCCAGGAT TTCCAGGTAA CAACGGTGCC 
CCAGGACTTC CAGGTGACCA TGGTGACATT GGACTGATGG GATTTCCAGG ATCACGTGGT 
TTCCCAGGAC CAAAGGGCTC TAAGGGAGTT ACAGGTTTCA AAGGACTACC AGGTGACCAG 
GGTGATGTTG GCTCACCAGG AATGCCTGGA AACCCAGGAC TGACAGGCCC CAAAGGACCT 
AAAGGAAAGA GAGGTGACCC AACTCTTCTG CTTGGTACAC AAGGTCAGAA AGGGCCTCCT 
GGAGATCTAG GATTACCAGG ATTCTGTGGA TTCCCAGGTG AGAAAGGTTC ACCAGGTATC 
CGAGGGCAAC CTGGAAGACC AGGATCCAAG GGCGAGCCTG GATACCCAGG AATACCTGGA 
TTTCCAGGAG CTACAGGTCC TCCAGGATTG CCAGGAGAAC CTGGAGAAAA AGGGAAACAT 
GGAATTTTGG GACCTCCAGG GCTGCTAGGA TTAACTGGAT CACCAGGTAG AAAAGGTCTT 
CCTGGACTGC CTGGACTGGA TGGCTTGGAC GGACTAAAAG GTCAAAAAGG TTCTGCAGGA 
GCTCCAGGTC AAAGTGAAAC AGGGCGCCCA GGATACTCTG GAGAACCTGG ACCAAAAGGA 
GAGAGAGGTG AACCTGGATG GCCTGGTATT TCCATTCCAG GCACCCCTGG AGAAAGAGGA 
TTCCCAGGAT TCCCAGGTAG AAGAGGACCT GTTGGACCCA TTGGACCTAG GGGACCATCT 
CCCAGTAGTG CTCCTCCTGG TCTTCCAGGT GATCAAGGAC CACCTGGTTT AGATGGCATC 
AGAGGTCAGC CTGGGAATCC TGGTCCTCCT GGAGAGACTG TCTTTGTACG AGGAGATCCA 
GGTGACACCG GTATTCGAGG GGCACCAGGC ATTCCTGGAC ACCGAGGGCT ACAAGGAGCC 
AGAGGTCTGC CAGGAAATCA AGGAAGAGAA GGTCCAAAGG GTCCAATGGG AATCCATGGC 
TTACAGGGTC CACCTGGTGC TGTAGGACAA CCAGGAGACC AAGGTTTTCA AGGAAAACCT 
GGTCCTAAAG GTCCCACAGG GTTCATAGGT GAGCCTGGTG AACCAGGAAA AACAGATGAC 
TCATGTCCTG CAATCCCAGG GCCTCCAGGG GAAGCAGGAC ATAGAGGAGA TGATGGTTCT 
ATAGGATTAC CAGGGCCAAT AGGTCACCCT GGACCCCAAG GAAGGAAAGG TGAAGAAGGG 
TCATGTGGCC TGCCAGGTCA AGATGGCTTA CCAGGAGCAC CTGGACCACC AGGTGACCAA 
GGGCATGTGG GAGAACAAGG ATATGCTGGA CCACAAGGTC CACCTGGCCA GACTGGTATC 
CCAGGACCAC CAGGGCCCCA TGTTAGATCT TCAAGTGGAT TCTTGCTCGT CCTTCATAGT 
CAGTCAGACA GAGAACCACT TTGTCCACAG GGGATGCCAA AATTATGGAC TGGCTATAGT 
CTGCTGTACC TGGAAGGACA AGAGAAAGCT CATAATCAAG ATCTTGGTCT GGCAGGATCT 
TGTCTTCCTG TATTTAATAC CATGCCCTTT GCTTACTGTA ACATCAACCA AGTTTGTTAC 
TATGCCAGCC GAAATGATAA GTCCTACTGG TTGTCAAGTG CCGCTCCCTT ACCAATGACA 
CCACTGTCTG AGGAAGAAAT ACAACCATAT ATAAGCAGAT GTGCTGTATG TGAGGCCCCA 
GCCCAAGCAG TAGCAGTTCA CAGCCAAGAT CAGTCAATTC CTCCCTGCCC AATGAACTGG 
AGAAGTCTTT GGATAGGATA TTCTTTTCTG ATGCACACTG GATCTGGTGA TCAGGGAGGT 
GGGCAGTCCC TTATGTCACC AGGAAGCTGC TTAGAAGACT TCAGATCAGC ACCGTTCATT 
GAATGCCAGG GTCAGCGGGG AACTTGTCAG TTCTTTGCTA ATGAATACAG CTTTTGGCTG 
ACAACAGTGA TGCCTGACTT GCAGTTTGCA TCAGCCCCCT TGTCAGGAAC TCTTAAAGAA 
GGACAAGAGC AGAGGAAAAA AATCAGTAGA TGCCAGGTAT GCCTGAAGCA TGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_015276986.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015132472.1 Gallus gallus collagen type IV alpha 4 chain (COL4A4), transcript variant X1, mRNA.

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CloneID	OGa39728
Clone ID Related Accession (Same CDS sequence)	XM_015276986.1 , XM_015276987.1 , XM_015276987.2 , XM_015276986.2
Accession Version	XM_015276986.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	collagen alpha-4(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015276986.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTTAA CAAAGGATTC ATTTGAACGA ATTAAGTGGC TTATCAGAAC TGCCTGGTGG 
CTGCTGATTG TTTTTTCAGC ACAAGAAATT GATGGGGGAG GATACGCATA TATTGACCCA 
TGTGGGGGAC AGGATTGTTC GGTTTGTCGG TGCTTTCCAG AAAAAGGATC AAGAGGTCAA 
CCTGGTGAGC TGGGAGCTCA AGGTCCAATT GGATCGTTAG GACCTACAGG ACCAGCTGGT 
CTGCCAGGGG AAAAAGGACA GAGAGGTGAG AATGGGCAGC CTGGGCCAGC TGGAGAAAAA 
GGTGATAAGG GCCCAACTGG AGTCCCAGGA TTTCCTGGTT TAGATGGTGT TCCTGGATTG 
CCAGGAAGTG AAGGACCCAG GGGCAAGCAC GGTGTAGATG GCTGCAATGG CTCAAGAGGA 
GATCCAGGAT TTCCAGGCGA AAATGGATAT ATTGGACCAA GAGGTCCTTA CGGAAACCCA 
GGGCAAAAAG GAGAAAAAGG AAATTCAGTG TATGTTTCGT ATTATGGAAA AGGACCCCCA 
GGAGACAGAG GTGATCCTGG CCCACCTGGT ATGCCCGGAC CTAGAGGATC GGCAGGTACA 
ACAGGCCCAC CTGGATACCC AGGCCAACCA GGCATGCCCG GTATTCCAGG TTACCCTGGT 
TTGCCAGGTG AACAGGGAAA TCCAGGTATT GGAGTGGATG GACAAAAGGG GGAGCCGGGT 
GATGTTGGAC TTCCCGGGCC ACCTGGGTCA CCACTGTTAG TTGGACCTCC TGGAGCTCAG 
CTGTTCAAAG GAGAAAAGGG CCAAAAAGGA CTACCTGGGA TAACGGGATA CAGAGGACCA 
CATGGACCTA AAGGAATACC TGGGAAAGGA GAAAAGGGTG AAAAAGGCAT TCCTGGATTC 
CCTGGATTGC GGGGTAGCCC TGGTTCTCAT GGACCTGCAG GCTTTCCTGG AATAAAGGGA 
GAAATGGGTG TTGCTGGTTT TCCTGGGAAA CCTGGCTATC CAGGCATCAA GGGGGATCCT 
GGAGAAAAAG GGCTCCCCGG TCCTCCTGGA ACTCCACTGC TTCCTATGAA AGGCCCACCA 
GGAGATCCTG GATTTCCAGG TCCTGCGGGT GACATGGGGT CAGTTGGACC TTCTGGCCCT 
GTGGGTATGC TAGGAAGACC AGGAGACGAT GGAACAAGTC TGCCTGGCCT GCCAGGAATA 
AGTGGGGCAC CAGGACCTCA AGGTTTTCAG GGTGACCCTG GTTTGCCTGG AACAGGTGAA 
GCAATTCCAG GAAGACCTGG ATTTCCTGGA CCACCTGGAC TACCAGGACA GCCAGGAAGA 
CAAGGCTTAC CTGGACTACC TAGTGTAATT TGTACTGAGA GAGGGATTCC TGGGGAACCT 
GGAGCAAAAG GTCAGATAGG CCTTCCGGGT AGAAAAGGTG AAAAAGGAGA AAAAGGAAAT 
CAAGGCCTCT GCTCATGTAG TGCTGGTCCT CCTGGTCCCC GTGGTATACA AGGACCTTCA 
GGTACTCAAG GGAAGAAAGG ACAAATGGGT TACCCTGGAA GACGTGGAGA AAAGGGTGAT 
TCAGGCTTAT CTGGTGCAGT GGGATCACCA GGACTCCCTG GGACACCTGG TTCTGGAGGA 
CAACATGGTG AAAAAGGAGA GAAGGGTGAC CAGGGAAGAG TTTCAATAAA AGGAATAAAA 
GGTGAAAGAG GTCCTACTGG GGTTCCAGGA TTTCCAGGCC AAAAAGGTAA TGATGGCAGA 
GATGGGGATT TAGGATTGCC TGGGGAAAAA GGAGATGAGG GTGATTCTGG AGTACCAATA 
CCAGGTGATA AAGGATTTCC TGGAGTTCCA GGACTCCCTG GAGTAAAAGG ACCAATGGGA 
TTGCCTGGTT TGGGGTTTCC TGGCCCTCCG GGAGTTAGAG GTACCCCTGG AGACTTTGGT 
GATGATGGTA ACATTGGTCT GCCTGGTCCA AAGGGTCAGA AAGGTGACAC TATCTGTATT 
ACATCACCGT ACCCTGGAAA CCCAGGTCCC CCAGGTTTTA AAGGTGTTCA AGGACCAAAG 
GGTTTAAAAG GACTCCCTGG TCTTCCTGGA CGAAGTGGCT TTGATGGGCC AAAAGGCCAG 
CAAGGCAGGC CAGGAGCAGG AATCCCTGGA CCAGAAGGCT TCCGAGGCAC AGCTGGTGAT 
CCTGGTGATG AAGGTGAAAG AGGATCTACC ATTGATGGTA AAAATGGTGC TCCAGGTCCA 
CCTGGCATAG ATGGCCAGAA GGGTGTTTCA GGTGATACTA CATATGGCCC TCCAGGATTC 
CCAGGCAACA GGGGACTGCC AGGACCACCT GGTGCACAAG GAGCAAGGGG TGATCCAGGC 
ATTCCTGGGC TTCAAGGGCA TCCTGGGACC CCAGGATTTC CAGGTGCTAA AGGTTTTAGA 
GGTCCAGAAG GTGATACAGG AGCGCCAGGC TGTCCAGGCA CCCCTGGTCC ACCATGTGAC 
ACACAATTAC CAGGGCCACC TGGACTCAGA GGTGTCATAG GCCTGCCAGG TTCTCAAGGC 
TTACCAGGCT TTAAAGGTCA GAAAGGAGAC AGGGGCCTAG CTGGGCCACC TGGAATCAAG 
GGTCTCAAGG GCTCTCCTGG CTCACAGGGC CCCCCAGGTC CTCCAGGGTC CCGAGGACTT 
CCAGGACTAC CAGGCAATAA AGGACAACCT GGCTTTCCAG GTCAAACAGG AAGCAAAGGG 
ATTCAAGGAC CCCAAGGATT CCCTGGACTC CCAGGAACAC AAGGCCAAAT GGGAATTCCT 
GGTGTAAAAG GGGAAGAAGG AAACATGGGT CCACCTGGGC CTAGTGGAGA ATGTGGGGAT 
ATGGGAATGA AAGGTGAAAG AGGGCTTCCA GGAGGTGGTG GCAGCATTAA CATTCGGCTT 
GAGAAAGGAC AAAAAGGAGA ACCAGGGTTT CCTGGTGAGA ATGGATTTAG AGGTGAAAGA 
GGTGAAAAAG GAAATACAGG TTTCAGAGGT GCCCCAGGAT TTCCAGGTAA CAACGGTGCC 
CCAGGACTTC CAGGTGACCA TGGTGACATT GGACTGATGG GATTTCCAGG ATCACGTGGT 
TTCCCAGGAC CAAAGGGCTC TAAGGGAGTT ACAGGTTTCA AAGGACTACC AGGTGACCAG 
GGTGATGTTG GCTCACCAGG AATGCCTGGA AACCCAGGAC TGACAGGCCC CAAAGGACCT 
AAAGGAAAGA GAGGTGACCC AACTCTTCTG CTTGGTACAC AAGGTCAGAA AGGGCCTCCT 
GGAGATCTAG GATTACCAGG ATTCTGTGGA TTCCCAGGTG AGAAAGGTTC ACCAGGTATC 
CGAGGGCAAC CTGGAAGACC AGGATCCAAG GGCGAGCCTG GATACCCAGG AATACCTGGA 
TTTCCAGGAG CTACAGGTCC TCCAGGATTG CCAGGAGAAC CTGGAGAAAA AGGGAAACAT 
GGAATTTTGG GACCTCCAGG GCTGCTAGGA TTAACTGGAT CACCAGGTAG AAAAGGTCTT 
CCTGGACTGC CTGGACTGGA TGGCTTGGAC GGACTAAAAG GTCAAAAAGG TTCTGCAGGA 
GCTCCAGGTC AAAGTGAAAC AGGGCGCCCA GGATACTCTG GAGAACCTGG ACCAAAAGGA 
GAGAGAGGTG AACCTGGATG GCCTGGTATT TCCATTCCAG GCACCCCTGG AGAAAGAGGA 
TTCCCAGGAT TCCCAGGTAG AAGAGGACCT GTTGGACCCA TTGGACCTAG GGGACCATCT 
CCCAGTAGTG CTCCTCCTGG TCTTCCAGGT GATCAAGGAC CACCTGGTTT AGATGGCATC 
AGAGGTCAGC CTGGGAATCC TGGTCCTCCT GGAGAGACTG TCTTTGTACG AGGAGATCCA 
GGTGACACCG GTATTCGAGG GGCACCAGGC ATTCCTGGAC ACCGAGGGCT ACAAGGAGCC 
AGAGGTCTGC CAGGAAATCA AGGAAGAGAA GGTCCAAAGG GTCCAATGGG AATCCATGGC 
TTACAGGGTC CACCTGGTGC TGTAGGACAA CCAGGAGACC AAGGTTTTCA AGGAAAACCT 
GGTCCTAAAG GTCCCACAGG GTTCATAGGT GAGCCTGGTG AACCAGGAAA AACAGATGAC 
TCATGTCCTG CAATCCCAGG GCCTCCAGGG GAAGCAGGAC ATAGAGGAGA TGATGGTTCT 
ATAGGATTAC CAGGGCCAAT AGGTCACCCT GGACCCCAAG GAAGGAAAGG TGAAGAAGGG 
TCATGTGGCC TGCCAGGTCA AGATGGCTTA CCAGGAGCAC CTGGACCACC AGGTGACCAA 
GGGCATGTGG GAGAACAAGG ATATGCTGGA CCACAAGGTC CACCTGGCCA GACTGGTATC 
CCAGGACCAC CAGGGCCCCA TGTTAGATCT TCAAGTGGAT TCTTGCTCGT CCTTCATAGT 
CAGTCAGACA GAGAACCACT TTGTCCACAG GGGATGCCAA AATTATGGAC TGGCTATAGT 
CTGCTGTACC TGGAAGGACA AGAGAAAGCT CATAATCAAG ATCTTGGTCT GGCAGGATCT 
TGTCTTCCTG TATTTAATAC CATGCCCTTT GCTTACTGTA ACATCAACCA AGTTTGTTAC 
TATGCCAGCC GAAATGATAA GTCCTACTGG TTGTCAAGTG CCGCTCCCTT ACCAATGACA 
CCACTGTCTG AGGAAGAAAT ACAACCATAT ATAAGCAGAT GTGCTGTATG TGAGGCCCCA 
GCCCAAGCAG TAGCAGTTCA CAGCCAAGAT CAGTCAATTC CTCCCTGCCC AATGAACTGG 
AGAAGTCTTT GGATAGGATA TTCTTTTCTG ATGCACACTG GATCTGGTGA TCAGGGAGGT 
GGGCAGTCCC TTATGTCACC AGGAAGCTGC TTAGAAGACT TCAGATCAGC ACCGTTCATT 
GAATGCCAGG GTCAGCGGGG AACTTGTCAG TTCTTTGCTA ATGAATACAG CTTTTGGCTG 
ACAACAGTGA TGCCTGACTT GCAGTTTGCA TCAGCCCCCT TGTCAGGAAC TCTTAAAGAA 
GGACAAGAGC AGAGGAAAAA AATCAGTAGA TGCCAGGTAT GCCTGAAGCA TGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015132472.1
CDS	276..5312
Translation	MALTKDSFERIKWLIRTAWWLLIVFSAQEIDGGGYAYIDPCGGQDCSVCRCFPEKGSRGQPGELGAQGPIGSLGPTGPAGLPGEKGQRGENGQPGPAGEKGDKGPTGVPGFPGLDGVPGLPGSEGPRGKHGVDGCNGSRGDPGFPGENGYIGPRGPYGNPGQKGEKGNSVYVSYYGKGPPGDRGDPGPPGMPGPRGSAGTTGPPGYPGQPGMPGIPGYPGLPGEQGNPGIGVDGQKGEPGDVGLPGPPGSPLLVGPPGAQLFKGEKGQKGLPGITGYRGPHGPKGIPGKGEKGEKGIPGFPGLRGSPGSHGPAGFPGIKGEMGVAGFPGKPGYPGIKGDPGEKGLPGPPGTPLLPMKGPPGDPGFPGPAGDMGSVGPSGPVGMLGRPGDDGTSLPGLPGISGAPGPQGFQGDPGLPGTGEAIPGRPGFPGPPGLPGQPGRQGLPGLPSVICTERGIPGEPGAKGQIGLPGRKGEKGEKGNQGLCSCSAGPPGPRGIQGPSGTQGKKGQMGYPGRRGEKGDSGLSGAVGSPGLPGTPGSGGQHGEKGEKGDQGRVSIKGIKGERGPTGVPGFPGQKGNDGRDGDLGLPGEKGDEGDSGVPIPGDKGFPGVPGLPGVKGPMGLPGLGFPGPPGVRGTPGDFGDDGNIGLPGPKGQKGDTICITSPYPGNPGPPGFKGVQGPKGLKGLPGLPGRSGFDGPKGQQGRPGAGIPGPEGFRGTAGDPGDEGERGSTIDGKNGAPGPPGIDGQKGVSGDTTYGPPGFPGNRGLPGPPGAQGARGDPGIPGLQGHPGTPGFPGAKGFRGPEGDTGAPGCPGTPGPPCDTQLPGPPGLRGVIGLPGSQGLPGFKGQKGDRGLAGPPGIKGLKGSPGSQGPPGPPGSRGLPGLPGNKGQPGFPGQTGSKGIQGPQGFPGLPGTQGQMGIPGVKGEEGNMGPPGPSGECGDMGMKGERGLPGGGGSINIRLEKGQKGEPGFPGENGFRGERGEKGNTGFRGAPGFPGNNGAPGLPGDHGDIGLMGFPGSRGFPGPKGSKGVTGFKGLPGDQGDVGSPGMPGNPGLTGPKGPKGKRGDPTLLLGTQGQKGPPGDLGLPGFCGFPGEKGSPGIRGQPGRPGSKGEPGYPGIPGFPGATGPPGLPGEPGEKGKHGILGPPGLLGLTGSPGRKGLPGLPGLDGLDGLKGQKGSAGAPGQSETGRPGYSGEPGPKGERGEPGWPGISIPGTPGERGFPGFPGRRGPVGPIGPRGPSPSSAPPGLPGDQGPPGLDGIRGQPGNPGPPGETVFVRGDPGDTGIRGAPGIPGHRGLQGARGLPGNQGREGPKGPMGIHGLQGPPGAVGQPGDQGFQGKPGPKGPTGFIGEPGEPGKTDDSCPAIPGPPGEAGHRGDDGSIGLPGPIGHPGPQGRKGEEGSCGLPGQDGLPGAPGPPGDQGHVGEQGYAGPQGPPGQTGIPGPPGPHVRSSSGFLLVLHSQSDREPLCPQGMPKLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFNTMPFAYCNINQVCYYASRNDKSYWLSSAAPLPMTPLSEEEIQPYISRCAVCEAPAQAVAVHSQDQSIPPCPMNWRSLWIGYSFLMHTGSGDQGGGQSLMSPGSCLEDFRSAPFIECQGQRGTCQFFANEYSFWLTTVMPDLQFASAPLSGTLKEGQEQRKKISRCQVCLKHD

Target ORF information:

RefSeq Version	XM_015276986.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus collagen type IV alpha 4 chain (COL4A4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276986.2

ORF Insert Sequence:

ATGGCGTTAA CAAAGGATTC ATTTGAACGA ATTAAGTGGC TTATCAGAAC TGCCTGGTGG 
CTGCTGATTG TTTTTTCAGC ACAAGAAATT GATGGGGGAG GATACGCATA TATTGACCCA 
TGTGGGGGAC AGGATTGTTC GGTTTGTCGG TGCTTTCCAG AAAAAGGATC AAGAGGTCAA 
CCTGGTGAGC TGGGAGCTCA AGGTCCAATT GGATCGTTAG GACCTACAGG ACCAGCTGGT 
CTGCCAGGGG AAAAAGGACA GAGAGGTGAG AATGGGCAGC CTGGGCCAGC TGGAGAAAAA 
GGTGATAAGG GCCCAACTGG AGTCCCAGGA TTTCCTGGTT TAGATGGTGT TCCTGGATTG 
CCAGGAAGTG AAGGACCCAG GGGCAAGCAC GGTGTAGATG GCTGCAATGG CTCAAGAGGA 
GATCCAGGAT TTCCAGGCGA AAATGGATAT ATTGGACCAA GAGGTCCTTA CGGAAACCCA 
GGGCAAAAAG GAGAAAAAGG AAATTCAGTG TATGTTTCGT ATTATGGAAA AGGACCCCCA 
GGAGACAGAG GTGATCCTGG CCCACCTGGT ATGCCCGGAC CTAGAGGATC GGCAGGTACA 
ACAGGCCCAC CTGGATACCC AGGCCAACCA GGCATGCCCG GTATTCCAGG TTACCCTGGT 
TTGCCAGGTG AACAGGGAAA TCCAGGTATT GGAGTGGATG GACAAAAGGG GGAGCCGGGT 
GATGTTGGAC TTCCCGGGCC ACCTGGGTCA CCACTGTTAG TTGGACCTCC TGGAGCTCAG 
CTGTTCAAAG GAGAAAAGGG CCAAAAAGGA CTACCTGGGA TAACGGGATA CAGAGGACCA 
CATGGACCTA AAGGAATACC TGGGAAAGGA GAAAAGGGTG AAAAAGGCAT TCCTGGATTC 
CCTGGATTGC GGGGTAGCCC TGGTTCTCAT GGACCTGCAG GCTTTCCTGG AATAAAGGGA 
GAAATGGGTG TTGCTGGTTT TCCTGGGAAA CCTGGCTATC CAGGCATCAA GGGGGATCCT 
GGAGAAAAAG GGCTCCCCGG TCCTCCTGGA ACTCCACTGC TTCCTATGAA AGGCCCACCA 
GGAGATCCTG GATTTCCAGG TCCTGCGGGT GACATGGGGT CAGTTGGACC TTCTGGCCCT 
GTGGGTATGC TAGGAAGACC AGGAGACGAT GGAACAAGTC TGCCTGGCCT GCCAGGAATA 
AGTGGGGCAC CAGGACCTCA AGGTTTTCAG GGTGACCCTG GTTTGCCTGG AACAGGTGAA 
GCAATTCCAG GAAGACCTGG ATTTCCTGGA CCACCTGGAC TACCAGGACA GCCAGGAAGA 
CAAGGCTTAC CTGGACTACC TAGTGTAATT TGTACTGAGA GAGGGATTCC TGGGGAACCT 
GGAGCAAAAG GTCAGATAGG CCTTCCGGGT AGAAAAGGTG AAAAAGGAGA AAAAGGAAAT 
CAAGGCCTCT GCTCATGTAG TGCTGGTCCT CCTGGTCCCC GTGGTATACA AGGACCTTCA 
GGTACTCAAG GGAAGAAAGG ACAAATGGGT TACCCTGGAA GACGTGGAGA AAAGGGTGAT 
TCAGGCTTAT CTGGTGCAGT GGGATCACCA GGACTCCCTG GGACACCTGG TTCTGGAGGA 
CAACATGGTG AAAAAGGAGA GAAGGGTGAC CAGGGAAGAG TTTCAATAAA AGGAATAAAA 
GGTGAAAGAG GTCCTACTGG GGTTCCAGGA TTTCCAGGCC AAAAAGGTAA TGATGGCAGA 
GATGGGGATT TAGGATTGCC TGGGGAAAAA GGAGATGAGG GTGATTCTGG AGTACCAATA 
CCAGGTGATA AAGGATTTCC TGGAGTTCCA GGACTCCCTG GAGTAAAAGG ACCAATGGGA 
TTGCCTGGTT TGGGGTTTCC TGGCCCTCCG GGAGTTAGAG GTACCCCTGG AGACTTTGGT 
GATGATGGTA ACATTGGTCT GCCTGGTCCA AAGGGTCAGA AAGGTGACAC TATCTGTATT 
ACATCACCGT ACCCTGGAAA CCCAGGTCCC CCAGGTTTTA AAGGTGTTCA AGGACCAAAG 
GGTTTAAAAG GACTCCCTGG TCTTCCTGGA CGAAGTGGCT TTGATGGGCC AAAAGGCCAG 
CAAGGCAGGC CAGGAGCAGG AATCCCTGGA CCAGAAGGCT TCCGAGGCAC AGCTGGTGAT 
CCTGGTGATG AAGGTGAAAG AGGATCTACC ATTGATGGTA AAAATGGTGC TCCAGGTCCA 
CCTGGCATAG ATGGCCAGAA GGGTGTTTCA GGTGATACTA CATATGGCCC TCCAGGATTC 
CCAGGCAACA GGGGACTGCC AGGACCACCT GGTGCACAAG GAGCAAGGGG TGATCCAGGC 
ATTCCTGGGC TTCAAGGGCA TCCTGGGACC CCAGGATTTC CAGGTGCTAA AGGTTTTAGA 
GGTCCAGAAG GTGATACAGG AGCGCCAGGC TGTCCAGGCA CCCCTGGTCC ACCATGTGAC 
ACACAATTAC CAGGGCCACC TGGACTCAGA GGTGTCATAG GCCTGCCAGG TTCTCAAGGC 
TTACCAGGCT TTAAAGGTCA GAAAGGAGAC AGGGGCCTAG CTGGGCCACC TGGAATCAAG 
GGTCTCAAGG GCTCTCCTGG CTCACAGGGC CCCCCAGGTC CTCCAGGGTC CCGAGGACTT 
CCAGGACTAC CAGGCAATAA AGGACAACCT GGCTTTCCAG GTCAAACAGG AAGCAAAGGG 
ATTCAAGGAC CCCAAGGATT CCCTGGACTC CCAGGAACAC AAGGCCAAAT GGGAATTCCT 
GGTGTAAAAG GGGAAGAAGG AAACATGGGT CCACCTGGGC CTAGTGGAGA ATGTGGGGAT 
ATGGGAATGA AAGGTGAAAG AGGGCTTCCA GGAGGTGGTG GCAGCATTAA CATTCGGCTT 
GAGAAAGGAC AAAAAGGAGA ACCAGGGTTT CCTGGTGAGA ATGGATTTAG AGGTGAAAGA 
GGTGAAAAAG GAAATACAGG TTTCAGAGGT GCCCCAGGAT TTCCAGGTAA CAACGGTGCC 
CCAGGACTTC CAGGTGACCA TGGTGACATT GGACTGATGG GATTTCCAGG ATCACGTGGT 
TTCCCAGGAC CAAAGGGCTC TAAGGGAGTT ACAGGTTTCA AAGGACTACC AGGTGACCAG 
GGTGATGTTG GCTCACCAGG AATGCCTGGA AACCCAGGAC TGACAGGCCC CAAAGGACCT 
AAAGGAAAGA GAGGTGACCC AACTCTTCTG CTTGGTACAC AAGGTCAGAA AGGGCCTCCT 
GGAGATCTAG GATTACCAGG ATTCTGTGGA TTCCCAGGTG AGAAAGGTTC ACCAGGTATC 
CGAGGGCAAC CTGGAAGACC AGGATCCAAG GGCGAGCCTG GATACCCAGG AATACCTGGA 
TTTCCAGGAG CTACAGGTCC TCCAGGATTG CCAGGAGAAC CTGGAGAAAA AGGGAAACAT 
GGAATTTTGG GACCTCCAGG GCTGCTAGGA TTAACTGGAT CACCAGGTAG AAAAGGTCTT 
CCTGGACTGC CTGGACTGGA TGGCTTGGAC GGACTAAAAG GTCAAAAAGG TTCTGCAGGA 
GCTCCAGGTC AAAGTGAAAC AGGGCGCCCA GGATACTCTG GAGAACCTGG ACCAAAAGGA 
GAGAGAGGTG AACCTGGATG GCCTGGTATT TCCATTCCAG GCACCCCTGG AGAAAGAGGA 
TTCCCAGGAT TCCCAGGTAG AAGAGGACCT GTTGGACCCA TTGGACCTAG GGGACCATCT 
CCCAGTAGTG CTCCTCCTGG TCTTCCAGGT GATCAAGGAC CACCTGGTTT AGATGGCATC 
AGAGGTCAGC CTGGGAATCC TGGTCCTCCT GGAGAGACTG TCTTTGTACG AGGAGATCCA 
GGTGACACCG GTATTCGAGG GGCACCAGGC ATTCCTGGAC ACCGAGGGCT ACAAGGAGCC 
AGAGGTCTGC CAGGAAATCA AGGAAGAGAA GGTCCAAAGG GTCCAATGGG AATCCATGGC 
TTACAGGGTC CACCTGGTGC TGTAGGACAA CCAGGAGACC AAGGTTTTCA AGGAAAACCT 
GGTCCTAAAG GTCCCACAGG GTTCATAGGT GAGCCTGGTG AACCAGGAAA AACAGATGAC 
TCATGTCCTG CAATCCCAGG GCCTCCAGGG GAAGCAGGAC ATAGAGGAGA TGATGGTTCT 
ATAGGATTAC CAGGGCCAAT AGGTCACCCT GGACCCCAAG GAAGGAAAGG TGAAGAAGGG 
TCATGTGGCC TGCCAGGTCA AGATGGCTTA CCAGGAGCAC CTGGACCACC AGGTGACCAA 
GGGCATGTGG GAGAACAAGG ATATGCTGGA CCACAAGGTC CACCTGGCCA GACTGGTATC 
CCAGGACCAC CAGGGCCCCA TGTTAGATCT TCAAGTGGAT TCTTGCTCGT CCTTCATAGT 
CAGTCAGACA GAGAACCACT TTGTCCACAG GGGATGCCAA AATTATGGAC TGGCTATAGT 
CTGCTGTACC TGGAAGGACA AGAGAAAGCT CATAATCAAG ATCTTGGTCT GGCAGGATCT 
TGTCTTCCTG TATTTAATAC CATGCCCTTT GCTTACTGTA ACATCAACCA AGTTTGTTAC 
TATGCCAGCC GAAATGATAA GTCCTACTGG TTGTCAAGTG CCGCTCCCTT ACCAATGACA 
CCACTGTCTG AGGAAGAAAT ACAACCATAT ATAAGCAGAT GTGCTGTATG TGAGGCCCCA 
GCCCAAGCAG TAGCAGTTCA CAGCCAAGAT CAGTCAATTC CTCCCTGCCC AATGAACTGG 
AGAAGTCTTT GGATAGGATA TTCTTTTCTG ATGCACACTG GATCTGGTGA TCAGGGAGGT 
GGGCAGTCCC TTATGTCACC AGGAAGCTGC TTAGAAGACT TCAGATCAGC ACCGTTCATT 
GAATGCCAGG GTCAGCGGGG AACTTGTCAG TTCTTTGCTA ATGAATACAG CTTTTGGCTG 
ACAACAGTGA TGCCTGACTT GCAGTTTGCA TCAGCCCCCT TGTCAGGAAC TCTTAAAGAA 
GGACAAGAGC AGAGGAAAAA AATCAGTAGA TGCCAGGTAT GCCTGAAGCA TGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_015276988.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015132474.1 Gallus gallus collagen, type IV, alpha 4 (COL4A4), transcript variant X3, mRNA.

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CloneID	OGa39729
Clone ID Related Accession (Same CDS sequence)	XM_015276988.1
Accession Version	XM_015276988.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	collagen alpha-4(IV) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471743.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTTAA CAAAGGATTC ATTTGAACGA ATTAAGTGGC TTATCAGAAC TGCCTGGTGG 
CTGCTGATTG TTTTTTCAGC ACAAGAAATT GATGGGGGAG GATACGCATA TATTGACCCA 
TGTGGGGGAC AGGATTGTTC GGTTTGTCGG TGCTTTCCAG AAAAAGGATC AAGAGGTCAA 
CCTGGTGAGC TGGGAGCTCA AGGTCCAATT GGATCGTTAG GACCTACAGG ACCAGCTGGT 
CTGCCAGGGG AAAAAGGACA GAGAGGTGAG AATGGGCAGC CTGGGCCAGC TGGAGAAAAA 
GGTGATAAGG GCCCAACTGG AGTCCCAGGA TTTCCTGGTT TAGATGGTGT TCCTGGATTG 
CCAGGAAGTG AAGGACCCAG GGGCAAGCAC GGTGTAGATG GCTGCAATGG CTCAAGAGGA 
GATCCAGGAT TTCCAGGCGA AAATGGATAT ATTGGACCAA GAGGTCCTTA CGGAAACCCA 
GGGCAAAAAG GAGAAAAAGG AAATTCAGTG TATGTTTCGT ATTATGGAAA AGGACCCCCA 
GGAGACAGAG GTGATCCTGG CCCACCTGGT ATGCCCGGAC CTAGAGGATC GGCAGGTACA 
ACAGGCCCAC CTGGATACCC AGGCCAACCA GGCATGCCCG GTATTCCAGG TTACCCTGGT 
TTGCCAGGTG AACAGGGAAA TCCAGGTATT GGAGTGGATG GACAAAAGGG GGAGCCGGGT 
GATGTTGGAC TTCCCGGGCC ACCTGGGTCA CCACTGTTAG TTGGACCTCC TGGAGCTCAG 
CTGTTCAAAG GAGAAAAGGG CCAAAAAGGA CTACCTGGGA TAACGGGATA CAGAGGACCA 
CATGGACCTA AAGGAATACC TGGGAAAGGA GAAAAGGGTG AAAAAGGCAT TCCTGGATTC 
CCTGGATTGC GGGGTAGCCC TGGTTCTCAT GGACCTGCAG GCTTTCCTGG AATAAAGGGA 
GAAATGGGTG TTGCTGGTTT TCCTGGGAAA CCTGGCTATC CAGGCATCAA GGGGGATCCT 
GGAGAAAAAG GGCTCCCCGG TCCTCCTGGA ACTCCACTGC TTCCTATGAA AGGCCCACCA 
GGAGATCCTG GATTTCCAGG TCCTGCGGGT GACATGGGGT CAGTTGGACC TTCTGGCCCT 
GTGGGTATGC TAGGAAGACC AGGAGACGAT GGAACAAGTC TGCCTGGCCT GCCAGGAATA 
AGTGGGGCAC CAGGACCTCA AGGTTTTCAG GGTGACCCTG GTTTGCCTGG AACAGGTGAA 
GCAATTCCAG GAAGACCTGG ATTTCCTGGA CCACCTGGAC TACCAGGACA GCCAGGAAGA 
CAAGGCTTAC CTGGACTACC TAGTGTAATT TGTACTGAGA GAGGGATTCC TGGGGAACCT 
GGAGCAAAAG GTCAGATAGG CCTTCCGGGT AGAAAAGGTG AAAAAGGAGA AAAAGGAAAT 
CAAGGCCTCT GCTCATGTAG TGCTGGTCCT CCTGGTCCCC GTGGTATACA AGGACCTTCA 
GGTACTCAAG GGAAGAAAGG ACAAATGGGT TACCCTGGAA GACGTGGAGA AAAGGGTGAT 
TCAGGCTTAT CTGGTGCAGT GGGATCACCA GGACTCCCTG GGACACCTGG TTCTGGAGGA 
CAACATGGTG AAAAAGGAGA GAAGGGTGAC CAGGGAAGAG TTTCAATAAA AGGAATAAAA 
GGTGAAAGAG GTCCTACTGG GGTTCCAGGA TTTCCAGGCC AAAAAGGTAA TGATGGCAGA 
GATGGGGATT TAGGATTGCC TGGGGAAAAA GGAGATGAGG GTGATTCTGG AGTACCAATA 
CCAGGTGATA AAGGATTTCC TGGAGTTCCA GGACTCCCTG GAGTAAAAGG ACCAATGGGA 
TTGCCTGGTT TGGGGTTTCC TGGCCCTCCG GGAGTTAGAG GTACCCCTGG AGACTTTGGT 
GATGATGGTA ACATTGGTCT GCCTGGTCCA AAGGGTCAGA AAGGTGACAC TATCTGTATT 
ACATCACCGT ACCCTGGAAA CCCAGGTCCC CCAGGTTTTA AAGGTGTTCA AGGACCAAAG 
GGTTTAAAAG GACTCCCTGG TCTTCCTGGA CGAAGTGGCT TTGATGGGCC AAAAGGCCAG 
CAAGGCAGGC CAGGAGCAGG AATCCCTGGA CCAGAAGGCT TCCGAGGCAC AGCTGGTGAT 
CCTGGTGATG AAGGTGAAAG AGGATCTACC ATTGATGGAT TCCCAGGCAA CAGGGGACTG 
CCAGGACCAC CTGGTGCACA AGGAGCAAGG GGTGATCCAG GCATTCCTGG GCTTCAAGGG 
CATCCTGGGA CCCCAGGATT TCCAGGTGCT AAAGGTTTTA GAGGTCCAGA AGGTGATACA 
GGAGCGCCAG GCTGTCCAGG CACCCCTGGT CCACCATGTG ACACACAATT ACCAGGGCCA 
CCTGGACTCA GAGGTGTCAT AGGCCTGCCA GGTTCTCAAG GCTTACCAGG CTTTAAAGGT 
CAGAAAGGAG ACAGGGGCCT AGCTGGGCCA CCTGGAATCA AGGGTCTCAA GGGCTCTCCT 
GGCTCACAGG GCCCCCCAGG TCCTCCAGGG TCCCGAGGAC TTCCAGGACT ACCAGGCAAT 
AAAGGACAAC CTGGCTTTCC AGGTCAAACA GGAAGCAAAG GGATTCAAGG ACCCCAAGGA 
TTCCCTGGAC TCCCAGGAAC ACAAGGCCAA ATGGGAATTC CTGGTGTAAA AGGGGAAGAA 
GGAAACATGG GTCCACCTGG GCCTAGTGGA GAATGTGGGG ATATGGGAAT GAAAGGTGAA 
AGAGGGCTTC CAGGAGGTGG TGGCAGCATT AACATTCGGC TTGAGAAAGG ACAAAAAGGA 
GAACCAGGGT TTCCTGGTGA GAATGGATTT AGAGGTGAAA GAGGTGAAAA AGGAAATACA 
GGTTTCAGAG GTGCCCCAGG ATTTCCAGGT AACAACGGTG CCCCAGGACT TCCAGGTGAC 
CATGGTGACA TTGGACTGAT GGGATTTCCA GGATCACGTG GTTTCCCAGG ACCAAAGGGC 
TCTAAGGGAG TTACAGGTTT CAAAGGACTA CCAGGTGACC AGGGTGATGT TGGCTCACCA 
GGAATGCCTG GAAACCCAGG ACTGACAGGC CCCAAAGGAC CTAAAGGAAA GAGAGGTGAC 
CCAACTCTTC TGCTTGGTAC ACAAGGTCAG AAAGGGCCTC CTGGAGATCT AGGATTACCA 
GGATTCTGTG GATTCCCAGG TGAGAAAGGT TCACCAGGTA TCCGAGGGCA ACCTGGAAGA 
CCAGGATCCA AGGGCGAGCC TGGATACCCA GGAATACCTG GATTTCCAGG AGCTACAGGT 
CCTCCAGGAT TGCCAGGAGA ACCTGGAGAA AAAGGGAAAC ATGGAATTTT GGGACCTCCA 
GGGCTGCTAG GATTAACTGG ATCACCAGGT AGAAAAGGTC TTCCTGGACT GCCTGGACTG 
GATGGCTTGG ACGGACTAAA AGGTCAAAAA GGTTCTGCAG GAGCTCCAGG TCAAAGTGAA 
ACAGGGCGCC CAGGATACTC TGGAGAACCT GGACCAAAAG GAGAGAGAGG TGAACCTGGA 
TGGCCTGGTA TTTCCATTCC AGGCACCCCT GGAGAAAGAG GATTCCCAGG ATTCCCAGGT 
AGAAGAGGAC CTGTTGGACC CATTGGACCT AGGGGACCAT CTCCCAGTAG TGCTCCTCCT 
GGTCTTCCAG GTGATCAAGG ACCACCTGGT TTAGATGGCA TCAGAGGTCA GCCTGGGAAT 
CCTGGTCCTC CTGGAGAGAC TGTCTTTGTA CGAGGAGATC CAGGTGACAC CGGTATTCGA 
GGGGCACCAG GCATTCCTGG ACACCGAGGG CTACAAGGAG CCAGAGGTCT GCCAGGAAAT 
CAAGGAAGAG AAGGTCCAAA GGGTCCAATG GGAATCCATG GCTTACAGGG TCCACCTGGT 
GCTGTAGGAC AACCAGGAGA CCAAGGTTTT CAAGGAAAAC CTGGTCCTAA AGGTCCCACA 
GGGTTCATAG GTGAGCCTGG TGAACCAGGA AAAACAGATG ACTCATGTCC TGCAATCCCA 
GGGCCTCCAG GGGAAGCAGG ACATAGAGGA GATGATGGTT CTATAGGATT ACCAGGGCCA 
ATAGGTCACC CTGGACCCCA AGGAAGGAAA GGTGAAGAAG GGTCATGTGG CCTGCCAGGT 
CAAGATGGCT TACCAGGAGC ACCTGGACCA CCAGGTGACC AAGGGCATGT GGGAGAACAA 
GGATATGCTG GACCACAAGG TCCACCTGGC CAGACTGGTA TCCCAGGACC ACCAGGGCCC 
CATGTTAGAT CTTCAAGTGG ATTCTTGCTC GTCCTTCATA GTCAGTCAGA CAGAGAACCA 
CTTTGTCCAC AGGGGATGCC AAAATTATGG ACTGGCTATA GTCTGCTGTA CCTGGAAGGA 
CAAGAGAAAG CTCATAATCA AGATCTTGGT CTGGCAGGAT CTTGTCTTCC TGTATTTAAT 
ACCATGCCCT TTGCTTACTG TAACATCAAC CAAGTTTGTT ACTATGCCAG CCGAAATGAT 
AAGTCCTACT GGTTGTCAAG TGCCGCTCCC TTACCAATGA CACCACTGTC TGAGGAAGAA 
ATACAACCAT ATATAAGCAG ATGTGCTGTA TGTGAGGCCC CAGCCCAAGC AGTAGCAGTT 
CACAGCCAAG ATCAGTCAAT TCCTCCCTGC CCAATGAACT GGAGAAGTCT TTGGATAGGA 
TATTCTTTTC TGATGCACAC TGGATCTGGT GATCAGGGAG GTGGGCAGTC CCTTATGTCA 
CCAGGAAGCT GCTTAGAAGA CTTCAGATCA GCACCGTTCA TTGAATGCCA GGGTCAGCGG 
GGAACTTGTC AGTTCTTTGC TAATGAATAC AGCTTTTGGC TGACAACAGT GATGCCTGAC 
TTGCAGTTTG CATCAGCCCC CTTGTCAGGA ACTCTTAAAG AAGGACAAGA GCAGAGGAAA 
AAAATCAGTA GATGCCAGGT ATGCCTGAAG CATGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015132474.1
CDS	237..5195
Translation	MALTKDSFERIKWLIRTAWWLLIVFSAQEIDGGGYAYIDPCGGQDCSVCRCFPEKGSRGQPGELGAQGPIGSLGPTGPAGLPGEKGQRGENGQPGPAGEKGDKGPTGVPGFPGLDGVPGLPGSEGPRGKHGVDGCNGSRGDPGFPGENGYIGPRGPYGNPGQKGEKGNSVYVSYYGKGPPGDRGDPGPPGMPGPRGSAGTTGPPGYPGQPGMPGIPGYPGLPGEQGNPGIGVDGQKGEPGDVGLPGPPGSPLLVGPPGAQLFKGEKGQKGLPGITGYRGPHGPKGIPGKGEKGEKGIPGFPGLRGSPGSHGPAGFPGIKGEMGVAGFPGKPGYPGIKGDPGEKGLPGPPGTPLLPMKGPPGDPGFPGPAGDMGSVGPSGPVGMLGRPGDDGTSLPGLPGISGAPGPQGFQGDPGLPGTGEAIPGRPGFPGPPGLPGQPGRQGLPGLPSVICTERGIPGEPGAKGQIGLPGRKGEKGEKGNQGLCSCSAGPPGPRGIQGPSGTQGKKGQMGYPGRRGEKGDSGLSGAVGSPGLPGTPGSGGQHGEKGEKGDQGRVSIKGIKGERGPTGVPGFPGQKGNDGRDGDLGLPGEKGDEGDSGVPIPGDKGFPGVPGLPGVKGPMGLPGLGFPGPPGVRGTPGDFGDDGNIGLPGPKGQKGDTICITSPYPGNPGPPGFKGVQGPKGLKGLPGLPGRSGFDGPKGQQGRPGAGIPGPEGFRGTAGDPGDEGERGSTIDGFPGNRGLPGPPGAQGARGDPGIPGLQGHPGTPGFPGAKGFRGPEGDTGAPGCPGTPGPPCDTQLPGPPGLRGVIGLPGSQGLPGFKGQKGDRGLAGPPGIKGLKGSPGSQGPPGPPGSRGLPGLPGNKGQPGFPGQTGSKGIQGPQGFPGLPGTQGQMGIPGVKGEEGNMGPPGPSGECGDMGMKGERGLPGGGGSINIRLEKGQKGEPGFPGENGFRGERGEKGNTGFRGAPGFPGNNGAPGLPGDHGDIGLMGFPGSRGFPGPKGSKGVTGFKGLPGDQGDVGSPGMPGNPGLTGPKGPKGKRGDPTLLLGTQGQKGPPGDLGLPGFCGFPGEKGSPGIRGQPGRPGSKGEPGYPGIPGFPGATGPPGLPGEPGEKGKHGILGPPGLLGLTGSPGRKGLPGLPGLDGLDGLKGQKGSAGAPGQSETGRPGYSGEPGPKGERGEPGWPGISIPGTPGERGFPGFPGRRGPVGPIGPRGPSPSSAPPGLPGDQGPPGLDGIRGQPGNPGPPGETVFVRGDPGDTGIRGAPGIPGHRGLQGARGLPGNQGREGPKGPMGIHGLQGPPGAVGQPGDQGFQGKPGPKGPTGFIGEPGEPGKTDDSCPAIPGPPGEAGHRGDDGSIGLPGPIGHPGPQGRKGEEGSCGLPGQDGLPGAPGPPGDQGHVGEQGYAGPQGPPGQTGIPGPPGPHVRSSSGFLLVLHSQSDREPLCPQGMPKLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFNTMPFAYCNINQVCYYASRNDKSYWLSSAAPLPMTPLSEEEIQPYISRCAVCEAPAQAVAVHSQDQSIPPCPMNWRSLWIGYSFLMHTGSGDQGGGQSLMSPGSCLEDFRSAPFIECQGQRGTCQFFANEYSFWLTTVMPDLQFASAPLSGTLKEGQEQRKKISRCQVCLKHD

Target ORF information:

RefSeq Version	XM_015276988.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus collagen, type IV, alpha 4 (COL4A4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276988.1

ORF Insert Sequence:

ATGGCGTTAA CAAAGGATTC ATTTGAACGA ATTAAGTGGC TTATCAGAAC TGCCTGGTGG 
CTGCTGATTG TTTTTTCAGC ACAAGAAATT GATGGGGGAG GATACGCATA TATTGACCCA 
TGTGGGGGAC AGGATTGTTC GGTTTGTCGG TGCTTTCCAG AAAAAGGATC AAGAGGTCAA 
CCTGGTGAGC TGGGAGCTCA AGGTCCAATT GGATCGTTAG GACCTACAGG ACCAGCTGGT 
CTGCCAGGGG AAAAAGGACA GAGAGGTGAG AATGGGCAGC CTGGGCCAGC TGGAGAAAAA 
GGTGATAAGG GCCCAACTGG AGTCCCAGGA TTTCCTGGTT TAGATGGTGT TCCTGGATTG 
CCAGGAAGTG AAGGACCCAG GGGCAAGCAC GGTGTAGATG GCTGCAATGG CTCAAGAGGA 
GATCCAGGAT TTCCAGGCGA AAATGGATAT ATTGGACCAA GAGGTCCTTA CGGAAACCCA 
GGGCAAAAAG GAGAAAAAGG AAATTCAGTG TATGTTTCGT ATTATGGAAA AGGACCCCCA 
GGAGACAGAG GTGATCCTGG CCCACCTGGT ATGCCCGGAC CTAGAGGATC GGCAGGTACA 
ACAGGCCCAC CTGGATACCC AGGCCAACCA GGCATGCCCG GTATTCCAGG TTACCCTGGT 
TTGCCAGGTG AACAGGGAAA TCCAGGTATT GGAGTGGATG GACAAAAGGG GGAGCCGGGT 
GATGTTGGAC TTCCCGGGCC ACCTGGGTCA CCACTGTTAG TTGGACCTCC TGGAGCTCAG 
CTGTTCAAAG GAGAAAAGGG CCAAAAAGGA CTACCTGGGA TAACGGGATA CAGAGGACCA 
CATGGACCTA AAGGAATACC TGGGAAAGGA GAAAAGGGTG AAAAAGGCAT TCCTGGATTC 
CCTGGATTGC GGGGTAGCCC TGGTTCTCAT GGACCTGCAG GCTTTCCTGG AATAAAGGGA 
GAAATGGGTG TTGCTGGTTT TCCTGGGAAA CCTGGCTATC CAGGCATCAA GGGGGATCCT 
GGAGAAAAAG GGCTCCCCGG TCCTCCTGGA ACTCCACTGC TTCCTATGAA AGGCCCACCA 
GGAGATCCTG GATTTCCAGG TCCTGCGGGT GACATGGGGT CAGTTGGACC TTCTGGCCCT 
GTGGGTATGC TAGGAAGACC AGGAGACGAT GGAACAAGTC TGCCTGGCCT GCCAGGAATA 
AGTGGGGCAC CAGGACCTCA AGGTTTTCAG GGTGACCCTG GTTTGCCTGG AACAGGTGAA 
GCAATTCCAG GAAGACCTGG ATTTCCTGGA CCACCTGGAC TACCAGGACA GCCAGGAAGA 
CAAGGCTTAC CTGGACTACC TAGTGTAATT TGTACTGAGA GAGGGATTCC TGGGGAACCT 
GGAGCAAAAG GTCAGATAGG CCTTCCGGGT AGAAAAGGTG AAAAAGGAGA AAAAGGAAAT 
CAAGGCCTCT GCTCATGTAG TGCTGGTCCT CCTGGTCCCC GTGGTATACA AGGACCTTCA 
GGTACTCAAG GGAAGAAAGG ACAAATGGGT TACCCTGGAA GACGTGGAGA AAAGGGTGAT 
TCAGGCTTAT CTGGTGCAGT GGGATCACCA GGACTCCCTG GGACACCTGG TTCTGGAGGA 
CAACATGGTG AAAAAGGAGA GAAGGGTGAC CAGGGAAGAG TTTCAATAAA AGGAATAAAA 
GGTGAAAGAG GTCCTACTGG GGTTCCAGGA TTTCCAGGCC AAAAAGGTAA TGATGGCAGA 
GATGGGGATT TAGGATTGCC TGGGGAAAAA GGAGATGAGG GTGATTCTGG AGTACCAATA 
CCAGGTGATA AAGGATTTCC TGGAGTTCCA GGACTCCCTG GAGTAAAAGG ACCAATGGGA 
TTGCCTGGTT TGGGGTTTCC TGGCCCTCCG GGAGTTAGAG GTACCCCTGG AGACTTTGGT 
GATGATGGTA ACATTGGTCT GCCTGGTCCA AAGGGTCAGA AAGGTGACAC TATCTGTATT 
ACATCACCGT ACCCTGGAAA CCCAGGTCCC CCAGGTTTTA AAGGTGTTCA AGGACCAAAG 
GGTTTAAAAG GACTCCCTGG TCTTCCTGGA CGAAGTGGCT TTGATGGGCC AAAAGGCCAG 
CAAGGCAGGC CAGGAGCAGG AATCCCTGGA CCAGAAGGCT TCCGAGGCAC AGCTGGTGAT 
CCTGGTGATG AAGGTGAAAG AGGATCTACC ATTGATGGAT TCCCAGGCAA CAGGGGACTG 
CCAGGACCAC CTGGTGCACA AGGAGCAAGG GGTGATCCAG GCATTCCTGG GCTTCAAGGG 
CATCCTGGGA CCCCAGGATT TCCAGGTGCT AAAGGTTTTA GAGGTCCAGA AGGTGATACA 
GGAGCGCCAG GCTGTCCAGG CACCCCTGGT CCACCATGTG ACACACAATT ACCAGGGCCA 
CCTGGACTCA GAGGTGTCAT AGGCCTGCCA GGTTCTCAAG GCTTACCAGG CTTTAAAGGT 
CAGAAAGGAG ACAGGGGCCT AGCTGGGCCA CCTGGAATCA AGGGTCTCAA GGGCTCTCCT 
GGCTCACAGG GCCCCCCAGG TCCTCCAGGG TCCCGAGGAC TTCCAGGACT ACCAGGCAAT 
AAAGGACAAC CTGGCTTTCC AGGTCAAACA GGAAGCAAAG GGATTCAAGG ACCCCAAGGA 
TTCCCTGGAC TCCCAGGAAC ACAAGGCCAA ATGGGAATTC CTGGTGTAAA AGGGGAAGAA 
GGAAACATGG GTCCACCTGG GCCTAGTGGA GAATGTGGGG ATATGGGAAT GAAAGGTGAA 
AGAGGGCTTC CAGGAGGTGG TGGCAGCATT AACATTCGGC TTGAGAAAGG ACAAAAAGGA 
GAACCAGGGT TTCCTGGTGA GAATGGATTT AGAGGTGAAA GAGGTGAAAA AGGAAATACA 
GGTTTCAGAG GTGCCCCAGG ATTTCCAGGT AACAACGGTG CCCCAGGACT TCCAGGTGAC 
CATGGTGACA TTGGACTGAT GGGATTTCCA GGATCACGTG GTTTCCCAGG ACCAAAGGGC 
TCTAAGGGAG TTACAGGTTT CAAAGGACTA CCAGGTGACC AGGGTGATGT TGGCTCACCA 
GGAATGCCTG GAAACCCAGG ACTGACAGGC CCCAAAGGAC CTAAAGGAAA GAGAGGTGAC 
CCAACTCTTC TGCTTGGTAC ACAAGGTCAG AAAGGGCCTC CTGGAGATCT AGGATTACCA 
GGATTCTGTG GATTCCCAGG TGAGAAAGGT TCACCAGGTA TCCGAGGGCA ACCTGGAAGA 
CCAGGATCCA AGGGCGAGCC TGGATACCCA GGAATACCTG GATTTCCAGG AGCTACAGGT 
CCTCCAGGAT TGCCAGGAGA ACCTGGAGAA AAAGGGAAAC ATGGAATTTT GGGACCTCCA 
GGGCTGCTAG GATTAACTGG ATCACCAGGT AGAAAAGGTC TTCCTGGACT GCCTGGACTG 
GATGGCTTGG ACGGACTAAA AGGTCAAAAA GGTTCTGCAG GAGCTCCAGG TCAAAGTGAA 
ACAGGGCGCC CAGGATACTC TGGAGAACCT GGACCAAAAG GAGAGAGAGG TGAACCTGGA 
TGGCCTGGTA TTTCCATTCC AGGCACCCCT GGAGAAAGAG GATTCCCAGG ATTCCCAGGT 
AGAAGAGGAC CTGTTGGACC CATTGGACCT AGGGGACCAT CTCCCAGTAG TGCTCCTCCT 
GGTCTTCCAG GTGATCAAGG ACCACCTGGT TTAGATGGCA TCAGAGGTCA GCCTGGGAAT 
CCTGGTCCTC CTGGAGAGAC TGTCTTTGTA CGAGGAGATC CAGGTGACAC CGGTATTCGA 
GGGGCACCAG GCATTCCTGG ACACCGAGGG CTACAAGGAG CCAGAGGTCT GCCAGGAAAT 
CAAGGAAGAG AAGGTCCAAA GGGTCCAATG GGAATCCATG GCTTACAGGG TCCACCTGGT 
GCTGTAGGAC AACCAGGAGA CCAAGGTTTT CAAGGAAAAC CTGGTCCTAA AGGTCCCACA 
GGGTTCATAG GTGAGCCTGG TGAACCAGGA AAAACAGATG ACTCATGTCC TGCAATCCCA 
GGGCCTCCAG GGGAAGCAGG ACATAGAGGA GATGATGGTT CTATAGGATT ACCAGGGCCA 
ATAGGTCACC CTGGACCCCA AGGAAGGAAA GGTGAAGAAG GGTCATGTGG CCTGCCAGGT 
CAAGATGGCT TACCAGGAGC ACCTGGACCA CCAGGTGACC AAGGGCATGT GGGAGAACAA 
GGATATGCTG GACCACAAGG TCCACCTGGC CAGACTGGTA TCCCAGGACC ACCAGGGCCC 
CATGTTAGAT CTTCAAGTGG ATTCTTGCTC GTCCTTCATA GTCAGTCAGA CAGAGAACCA 
CTTTGTCCAC AGGGGATGCC AAAATTATGG ACTGGCTATA GTCTGCTGTA CCTGGAAGGA 
CAAGAGAAAG CTCATAATCA AGATCTTGGT CTGGCAGGAT CTTGTCTTCC TGTATTTAAT 
ACCATGCCCT TTGCTTACTG TAACATCAAC CAAGTTTGTT ACTATGCCAG CCGAAATGAT 
AAGTCCTACT GGTTGTCAAG TGCCGCTCCC TTACCAATGA CACCACTGTC TGAGGAAGAA 
ATACAACCAT ATATAAGCAG ATGTGCTGTA TGTGAGGCCC CAGCCCAAGC AGTAGCAGTT 
CACAGCCAAG ATCAGTCAAT TCCTCCCTGC CCAATGAACT GGAGAAGTCT TTGGATAGGA 
TATTCTTTTC TGATGCACAC TGGATCTGGT GATCAGGGAG GTGGGCAGTC CCTTATGTCA 
CCAGGAAGCT GCTTAGAAGA CTTCAGATCA GCACCGTTCA TTGAATGCCA GGGTCAGCGG 
GGAACTTGTC AGTTCTTTGC TAATGAATAC AGCTTTTGGC TGACAACAGT GATGCCTGAC 
TTGCAGTTTG CATCAGCCCC CTTGTCAGGA ACTCTTAAAG AAGGACAAGA GCAGAGGAAA 
AAAATCAGTA GATGCCAGGT ATGCCTGAAG CATGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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03-6811-6573
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オンライン注文

COL4A4 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報