TIE1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	TIE1
Entrez Gene ID	424562
Full Name	tyrosine kinase with immunoglobulin like and EGF like domains 1
General protein information	Preferred Names tyrosine kinase with immunoglobulin like and EGF like domains 1 Names tyrosine-protein kinase receptor Tie-1
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015291320.1	XP_015146806.1	tyrosine-protein kinase receptor Tie-1 isoform X1
XM_015291320.2	XP_015146806.1	tyrosine-protein kinase receptor Tie-1 isoform X1
XM_422400.5	XP_422400.4	tyrosine-protein kinase receptor Tie-1 isoform X2
XM_422400.6	XP_422400.4	tyrosine-protein kinase receptor Tie-1 isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	TIE1	NP_776390.1
Rattus norvegicus (Norway rat)	Tie1	NP_445997.1
Gallus gallus (chicken)	TIE1	XP_004936746.1
Danio rerio (zebrafish)	tie1	XP_001334673.5
Pan troglodytes (chimpanzee)	TIE1	XP_001173341.1
Canis lupus familiaris (dog)	TIE1	XP_539652.3
Mus musculus (house mouse)	Tie1	NP_035717.2
Xenopus tropicalis (tropical clawed frog)	tie1	XP_002931650.2
Homo sapiens (human)	TIE1	NP_005415.1

Gene Ontology

The following TIE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TIE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa39588	XM_015291320.2 Latest version!	Gallus gallus tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa12010	XM_422400.6 Latest version!	Gallus gallus tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OGa39588	XM_015291320.1	Gallus gallus tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (TIE1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa12010	XM_422400.5	Gallus gallus tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (TIE1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TIE1 ( XM_015291320.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015146806.1 Gallus gallus tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (TIE1), transcript variant X1, mRNA.

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CloneID	OGa39588
Clone ID Related Accession (Same CDS sequence)	XM_015291320.1 , XM_015291320.2
Accession Version	XM_015291320.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	tyrosine-protein kinase receptor Tie-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455913.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCTGGA TATTTTCCTG TGCCAGATTT GGAACACACT TCAAGGACAT GGGACTCCAG 
TTTTATCTAC TGCTTCTCCT CCCATGGATG GCAGGGGCCA TCCTAGACAT CACCCTGATT 
GCCAACGTGC AGAGCCTGTC CCACTCTGAC TTCTTCCTCT CCTGCATCAT GGGCGAGCGT 
GACGTGAGCT ACCTGCAGAT TGAGAGGGAG AACAAGATTG TGATGACGCA CCCCAAGACA 
GGCTTCCAAA ACTACCGCAA CCGCAGCAAC TACGTCCAGG CCAGGGGCTT CTCCATGCCT 
GACCTGGTGG GCATCCTCTA CTGCCTGGGG CGCACGCCAA CAGAGCAGGC CCAGGTGGTC 
TACGTGCACA ACAGCCACAA TGCCCACCTC TTCCCAGTGA AGGCCACGCA GTCTGTGAAT 
GTCGGCGAGC CGGCCACCTT CTCCGCCAGG GTCCTCAGGA GGAAGGAGAT GGATGTCATG 
TGGAAAAGAA ATGGTACCTA CTACCAGACC ACGGACCGGG GCGAGGTGCG GGGTGACCTT 
TTCACTCTAA CACTCCCGAA TGTCAGCGTA ACTGAAAATG GTGTCTACAG TGCCACTTTC 
ATGGGAGATA GCCCTCTGTG GAGTGCCTTC TACAGGCTAA TCGTCAGAGC GTGTGCTGCA 
AAGAAATGGG GTCCGTCCTG TGAGAAGGAC TGTCCTGACT GCCTGAATGG GGGCATCTGT 
CATGACCACG TCGGTGAATG CATCTGTCCT CCTGGCTTCA CAGGCACCCG TTGTGAGAGA 
GCCTGCCGGG AAGGCCAGTT TGGCCGCAAC TGTCAGGAGA CGTGCCAGAG AGCCCAGGGC 
TGCAGGGGGC TGAGCTTCTG CCTGCCGGAC CCCTATGGCT GCTCCTGCGC CTCGGGGTGG 
AGCGGCTCCC GCTGCAGCCA AGCCTGTCCC CCAGGATATT ATGGCCCCGA CTGCGCTCTG 
ATGTGTGCCT GCCGGAATGG TGGCAGCTGT AACCGCTTCA GTGGCTGTGT CTGCCCTGCA 
GGCTGGCATG GGCAGCACTG CGAGAAGTCA GACCGCTTCC CCCAGATCAT CAAACTGGCC 
TCGGAGCTGG AGTTCAACCT GGGCTCAGAG CCCATCATCA GCTGCGTGGC CACTGGCAAC 
CCACTGCCTG CAAGTGATGG TGTGGAGCTG CGCAAGGCTG ATGGCACCAT GCTCAAGCTG 
GTCAAAGCCA TCATAGAGTC GGGGCAGATC ACGTGCGAGT TTCAGGTGCA GCATCTGACG 
AAGGCCGACG CAGGGCTCTG GGAGTGCCGA GTCTCTACTA CTGGGGGCCA GGACAGCCGG 
AAAGTCAAGG TCAACATTCG AGTGCCACCA GCACCCCTGA GCCCCCCCCG GCTGCTGGCC 
AAGCAGAGTC GCCAGCTTGT GGTGTCACCT GTGGACTGCT TCTCTGGTGA TGGGCCCATT 
GTCTCTGTCA AGTTACTCTA CAAGCCCAAG GATGATGCCT CGGTGTGGTC GTCCATTGTG 
GTTGACAACA GTGAAAACAT CACCCTCATG AACCTCCGGC CGGTGACCGC CTATGTTGTC 
AAGGTACAGC TGAGCCGACC AGGGGATGGT GGTGAGGGCA GCAAGGGACC TGAGGCCGTC 
ATGGTGACTG AGTGCCTAGA GCCCACAGTC AAGCCTGTGA TTGAAGGTTG GTCCATTGAG 
GAGAAGAACA CCCTTCATGT CAACTGGAAA TTGCCGAGTA ACCATGAGCC AGCACATGGA 
TTCATCGTCC ATCTCTTCGA CTCCGCAAAG CAACTGGTCT ACAAGAAAAA CATCACGTCC 
ATTTCCGTAC TCTCTGCCCA CATTGGGGGC CTGGAGTTCA ACAAGGAGTA TGGGCTGGAG 
GTGCTGGTGT ACCACTGCAC CAGCCTGGGG CCCCCCTCTG ACCTCTACAA GGTCATGATC 
AACAGCAAAG GGCCTTCCTC CCCACGGTCA CTCTCAGCAG AGTCTGTGTC TGACACAGCC 
ATCAGGCTCT ACTGGCAGGT CCCCGAGTAC CCCAACGGGG GCATCACCAA GTACATTGTG 
GAGCTGCAGC AGGTGGGGGG GACCAGTGAG CCCCAGTGGA TTGACACCGA CAATGGCGCC 
GAGACCACCA AGATCATTGG AGGCCTCAAC GCCAGCACCA CCTACCAGTT TCGTGTCCGG 
GCCAACTCCC ATGTCCCAGG GGAGTGGAGC CAGCTGGTGA AAGCCAAAAC CCTGGGGGAT 
GGAGCACTGA GTGTGCCACC CAGCCTGGGC AGCCAGAGCA CCGAGCAGGC AGGAGTAGAC 
CAGCAGCTGC TCTTGGCCAT CATCGGCTCC GTGTCCGTCA CCTGCCTCAC CATCCTCTTT 
GCCCTCCTGG CACTTTTTCT CATCAAGAAG AACTTTTTCC ACCGGCGCCG CACCTTCACA 
TACCAGTCTG GCTCGGTGAG TGCTGCCTGC CCTGCCAGGA CAGTGTCCCC AGCTCCCCAC 
GTCCCAGCCC GATCCAGGTC ACGCTCCATA GCCTCCAGTC CGGATGCAGC CAGACCTCAC 
CTCCACACAG AGAGGGGAGA AGAGACCATC CTGCAGTTCA ATTCAGGGAC CCTGACCCTG 
ACGCGCCGGC CCAAGCCACA GCCCGAACCC CTCAGCTACC CCATCCTGGA GTGGGAGGAC 
ATCAAGTTTG AGGACATGAT TGGGGAAGGG AACTTTGGGC AGGTCATCAG GGCCATGATC 
AAAAAAGATG GCTTGAAAAT GAATGCAGCC ATCAAGATGC TGAAGGAGTT CGCCTCAGAG 
AATGACCACC GGGACTTTGC TGGAGAGTTA GAGGTGCTGT GCAAGCTGGG CCATCACCCC 
AACATAATCA ACTTGCTAGG CGCCTGTGAG AACAAGGGTT ACCTGTATAT TGCCATCGAG 
TATGCCCCCT ATGGAAACCT CCTCGACTTC CTCCGCAAAA GCCGAGTCCT GGAGACCGAC 
CCAGCCTTTG CCAAGGAGCA TGGCACCGCG TCCACCCTCA CCTCCCAGCA GCTCCTCCAG 
TTCGCTTCAG ACGTGGCCAA GGGGATGCAG TACCTAAGTG AGAAGCAGTT CATTCACAGG 
GACCTGGCAG CCAGGAATAT CCTGGTGGGA GAAAACCTGG CCTCCAAGAT CGCTGACTTT 
GGCCTTTCCA GGGGGGAGGA GGTCTACGTG AAGAAGACAA TGGGCCGCTT GCCAGTTCGT 
TGGATGGCCA TCGAGTCCCT CAACTACAGC GTGTACACCA CCAAGAGCGA TGTGTGGTCC 
TTTGGCGTCC TTCTCTGGGA GATCGTCAGC TTGGGGGGCA CGCCGTACTG CGGGATGACG 
TGTGCTGAGC TCTACGAGAA GCTGCCCCAG GGCTATCGCA TGGAGAAACC CCGCAACTGT 
GATGATGAGG TGTACGAGCT GATGAGGCAG TGCTGGCGCG ACCGTCCCTA CGAGCGCCCA 
CCCTTTGCCC AGATCTCCAT GCAGCTCATC CGCATGCTGG AGGCCAGGAA GGCATACGTG 
AACATGGCCC TGTTTGAGAA CTTCACCTAC GCAGGGATTG ATGCCACCGC GGAGGAGGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015146806.1
CDS	1362..4904
Translation	MCWIFSCARFGTHFKDMGLQFYLLLLLPWMAGAILDITLIANVQSLSHSDFFLSCIMGERDVSYLQIERENKIVMTHPKTGFQNYRNRSNYVQARGFSMPDLVGILYCLGRTPTEQAQVVYVHNSHNAHLFPVKATQSVNVGEPATFSARVLRRKEMDVMWKRNGTYYQTTDRGEVRGDLFTLTLPNVSVTENGVYSATFMGDSPLWSAFYRLIVRACAAKKWGPSCEKDCPDCLNGGICHDHVGECICPPGFTGTRCERACREGQFGRNCQETCQRAQGCRGLSFCLPDPYGCSCASGWSGSRCSQACPPGYYGPDCALMCACRNGGSCNRFSGCVCPAGWHGQHCEKSDRFPQIIKLASELEFNLGSEPIISCVATGNPLPASDGVELRKADGTMLKLVKAIIESGQITCEFQVQHLTKADAGLWECRVSTTGGQDSRKVKVNIRVPPAPLSPPRLLAKQSRQLVVSPVDCFSGDGPIVSVKLLYKPKDDASVWSSIVVDNSENITLMNLRPVTAYVVKVQLSRPGDGGEGSKGPEAVMVTECLEPTVKPVIEGWSIEEKNTLHVNWKLPSNHEPAHGFIVHLFDSAKQLVYKKNITSISVLSAHIGGLEFNKEYGLEVLVYHCTSLGPPSDLYKVMINSKGPSSPRSLSAESVSDTAIRLYWQVPEYPNGGITKYIVELQQVGGTSEPQWIDTDNGAETTKIIGGLNASTTYQFRVRANSHVPGEWSQLVKAKTLGDGALSVPPSLGSQSTEQAGVDQQLLLAIIGSVSVTCLTILFALLALFLIKKNFFHRRRTFTYQSGSVSAACPARTVSPAPHVPARSRSRSIASSPDAARPHLHTERGEETILQFNSGTLTLTRRPKPQPEPLSYPILEWEDIKFEDMIGEGNFGQVIRAMIKKDGLKMNAAIKMLKEFASENDHRDFAGELEVLCKLGHHPNIINLLGACENKGYLYIAIEYAPYGNLLDFLRKSRVLETDPAFAKEHGTASTLTSQQLLQFASDVAKGMQYLSEKQFIHRDLAARNILVGENLASKIADFGLSRGEEVYVKKTMGRLPVRWMAIESLNYSVYTTKSDVWSFGVLLWEIVSLGGTPYCGMTCAELYEKLPQGYRMEKPRNCDDEVYELMRQCWRDRPYERPPFAQISMQLIRMLEARKAYVNMALFENFTYAGIDATAEEA

Target ORF information:

RefSeq Version	XM_015291320.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (TIE1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291320.1

ORF Insert Sequence:

ATGTGCTGGA TATTTTCCTG TGCCAGATTT GGAACACACT TCAAGGACAT GGGACTCCAG 
TTTTATCTAC TGCTTCTCCT CCCATGGATG GCAGGGGCCA TCCTAGACAT CACCCTGATT 
GCCAACGTGC AGAGCCTGTC CCACTCTGAC TTCTTCCTCT CCTGCATCAT GGGCGAGCGT 
GACGTGAGCT ACCTGCAGAT TGAGAGGGAG AACAAGATTG TGATGACGCA CCCCAAGACA 
GGCTTCCAAA ACTACCGCAA CCGCAGCAAC TACGTCCAGG CCAGGGGCTT CTCCATGCCT 
GACCTGGTGG GCATCCTCTA CTGCCTGGGG CGCACGCCAA CAGAGCAGGC CCAGGTGGTC 
TACGTGCACA ACAGCCACAA TGCCCACCTC TTCCCAGTGA AGGCCACGCA GTCTGTGAAT 
GTCGGCGAGC CGGCCACCTT CTCCGCCAGG GTCCTCAGGA GGAAGGAGAT GGATGTCATG 
TGGAAAAGAA ATGGTACCTA CTACCAGACC ACGGACCGGG GCGAGGTGCG GGGTGACCTT 
TTCACTCTAA CACTCCCGAA TGTCAGCGTA ACTGAAAATG GTGTCTACAG TGCCACTTTC 
ATGGGAGATA GCCCTCTGTG GAGTGCCTTC TACAGGCTAA TCGTCAGAGC GTGTGCTGCA 
AAGAAATGGG GTCCGTCCTG TGAGAAGGAC TGTCCTGACT GCCTGAATGG GGGCATCTGT 
CATGACCACG TCGGTGAATG CATCTGTCCT CCTGGCTTCA CAGGCACCCG TTGTGAGAGA 
GCCTGCCGGG AAGGCCAGTT TGGCCGCAAC TGTCAGGAGA CGTGCCAGAG AGCCCAGGGC 
TGCAGGGGGC TGAGCTTCTG CCTGCCGGAC CCCTATGGCT GCTCCTGCGC CTCGGGGTGG 
AGCGGCTCCC GCTGCAGCCA AGCCTGTCCC CCAGGATATT ATGGCCCCGA CTGCGCTCTG 
ATGTGTGCCT GCCGGAATGG TGGCAGCTGT AACCGCTTCA GTGGCTGTGT CTGCCCTGCA 
GGCTGGCATG GGCAGCACTG CGAGAAGTCA GACCGCTTCC CCCAGATCAT CAAACTGGCC 
TCGGAGCTGG AGTTCAACCT GGGCTCAGAG CCCATCATCA GCTGCGTGGC CACTGGCAAC 
CCACTGCCTG CAAGTGATGG TGTGGAGCTG CGCAAGGCTG ATGGCACCAT GCTCAAGCTG 
GTCAAAGCCA TCATAGAGTC GGGGCAGATC ACGTGCGAGT TTCAGGTGCA GCATCTGACG 
AAGGCCGACG CAGGGCTCTG GGAGTGCCGA GTCTCTACTA CTGGGGGCCA GGACAGCCGG 
AAAGTCAAGG TCAACATTCG AGTGCCACCA GCACCCCTGA GCCCCCCCCG GCTGCTGGCC 
AAGCAGAGTC GCCAGCTTGT GGTGTCACCT GTGGACTGCT TCTCTGGTGA TGGGCCCATT 
GTCTCTGTCA AGTTACTCTA CAAGCCCAAG GATGATGCCT CGGTGTGGTC GTCCATTGTG 
GTTGACAACA GTGAAAACAT CACCCTCATG AACCTCCGGC CGGTGACCGC CTATGTTGTC 
AAGGTACAGC TGAGCCGACC AGGGGATGGT GGTGAGGGCA GCAAGGGACC TGAGGCCGTC 
ATGGTGACTG AGTGCCTAGA GCCCACAGTC AAGCCTGTGA TTGAAGGTTG GTCCATTGAG 
GAGAAGAACA CCCTTCATGT CAACTGGAAA TTGCCGAGTA ACCATGAGCC AGCACATGGA 
TTCATCGTCC ATCTCTTCGA CTCCGCAAAG CAACTGGTCT ACAAGAAAAA CATCACGTCC 
ATTTCCGTAC TCTCTGCCCA CATTGGGGGC CTGGAGTTCA ACAAGGAGTA TGGGCTGGAG 
GTGCTGGTGT ACCACTGCAC CAGCCTGGGG CCCCCCTCTG ACCTCTACAA GGTCATGATC 
AACAGCAAAG GGCCTTCCTC CCCACGGTCA CTCTCAGCAG AGTCTGTGTC TGACACAGCC 
ATCAGGCTCT ACTGGCAGGT CCCCGAGTAC CCCAACGGGG GCATCACCAA GTACATTGTG 
GAGCTGCAGC AGGTGGGGGG GACCAGTGAG CCCCAGTGGA TTGACACCGA CAATGGCGCC 
GAGACCACCA AGATCATTGG AGGCCTCAAC GCCAGCACCA CCTACCAGTT TCGTGTCCGG 
GCCAACTCCC ATGTCCCAGG GGAGTGGAGC CAGCTGGTGA AAGCCAAAAC CCTGGGGGAT 
GGAGCACTGA GTGTGCCACC CAGCCTGGGC AGCCAGAGCA CCGAGCAGGC AGGAGTAGAC 
CAGCAGCTGC TCTTGGCCAT CATCGGCTCC GTGTCCGTCA CCTGCCTCAC CATCCTCTTT 
GCCCTCCTGG CACTTTTTCT CATCAAGAAG AACTTTTTCC ACCGGCGCCG CACCTTCACA 
TACCAGTCTG GCTCGGTGAG TGCTGCCTGC CCTGCCAGGA CAGTGTCCCC AGCTCCCCAC 
GTCCCAGCCC GATCCAGGTC ACGCTCCATA GCCTCCAGTC CGGATGCAGC CAGACCTCAC 
CTCCACACAG AGAGGGGAGA AGAGACCATC CTGCAGTTCA ATTCAGGGAC CCTGACCCTG 
ACGCGCCGGC CCAAGCCACA GCCCGAACCC CTCAGCTACC CCATCCTGGA GTGGGAGGAC 
ATCAAGTTTG AGGACATGAT TGGGGAAGGG AACTTTGGGC AGGTCATCAG GGCCATGATC 
AAAAAAGATG GCTTGAAAAT GAATGCAGCC ATCAAGATGC TGAAGGAGTT CGCCTCAGAG 
AATGACCACC GGGACTTTGC TGGAGAGTTA GAGGTGCTGT GCAAGCTGGG CCATCACCCC 
AACATAATCA ACTTGCTAGG CGCCTGTGAG AACAAGGGTT ACCTGTATAT TGCCATCGAG 
TATGCCCCCT ATGGAAACCT CCTCGACTTC CTCCGCAAAA GCCGAGTCCT GGAGACCGAC 
CCAGCCTTTG CCAAGGAGCA TGGCACCGCG TCCACCCTCA CCTCCCAGCA GCTCCTCCAG 
TTCGCTTCAG ACGTGGCCAA GGGGATGCAG TACCTAAGTG AGAAGCAGTT CATTCACAGG 
GACCTGGCAG CCAGGAATAT CCTGGTGGGA GAAAACCTGG CCTCCAAGAT CGCTGACTTT 
GGCCTTTCCA GGGGGGAGGA GGTCTACGTG AAGAAGACAA TGGGCCGCTT GCCAGTTCGT 
TGGATGGCCA TCGAGTCCCT CAACTACAGC GTGTACACCA CCAAGAGCGA TGTGTGGTCC 
TTTGGCGTCC TTCTCTGGGA GATCGTCAGC TTGGGGGGCA CGCCGTACTG CGGGATGACG 
TGTGCTGAGC TCTACGAGAA GCTGCCCCAG GGCTATCGCA TGGAGAAACC CCGCAACTGT 
GATGATGAGG TGTACGAGCT GATGAGGCAG TGCTGGCGCG ACCGTCCCTA CGAGCGCCCA 
CCCTTTGCCC AGATCTCCAT GCAGCTCATC CGCATGCTGG AGGCCAGGAA GGCATACGTG 
AACATGGCCC TGTTTGAGAA CTTCACCTAC GCAGGGATTG ATGCCACCGC GGAGGAGGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIE1 ( XM_015291320.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015146806.1 Gallus gallus tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa39588
Clone ID Related Accession (Same CDS sequence)	XM_015291320.1 , XM_015291320.2
Accession Version	XM_015291320.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	tyrosine-protein kinase receptor Tie-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291320.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCTGGA TATTTTCCTG TGCCAGATTT GGAACACACT TCAAGGACAT GGGACTCCAG 
TTTTATCTAC TGCTTCTCCT CCCATGGATG GCAGGGGCCA TCCTAGACAT CACCCTGATT 
GCCAACGTGC AGAGCCTGTC CCACTCTGAC TTCTTCCTCT CCTGCATCAT GGGCGAGCGT 
GACGTGAGCT ACCTGCAGAT TGAGAGGGAG AACAAGATTG TGATGACGCA CCCCAAGACA 
GGCTTCCAAA ACTACCGCAA CCGCAGCAAC TACGTCCAGG CCAGGGGCTT CTCCATGCCT 
GACCTGGTGG GCATCCTCTA CTGCCTGGGG CGCACGCCAA CAGAGCAGGC CCAGGTGGTC 
TACGTGCACA ACAGCCACAA TGCCCACCTC TTCCCAGTGA AGGCCACGCA GTCTGTGAAT 
GTCGGCGAGC CGGCCACCTT CTCCGCCAGG GTCCTCAGGA GGAAGGAGAT GGATGTCATG 
TGGAAAAGAA ATGGTACCTA CTACCAGACC ACGGACCGGG GCGAGGTGCG GGGTGACCTT 
TTCACTCTAA CACTCCCGAA TGTCAGCGTA ACTGAAAATG GTGTCTACAG TGCCACTTTC 
ATGGGAGATA GCCCTCTGTG GAGTGCCTTC TACAGGCTAA TCGTCAGAGC GTGTGCTGCA 
AAGAAATGGG GTCCGTCCTG TGAGAAGGAC TGTCCTGACT GCCTGAATGG GGGCATCTGT 
CATGACCACG TCGGTGAATG CATCTGTCCT CCTGGCTTCA CAGGCACCCG TTGTGAGAGA 
GCCTGCCGGG AAGGCCAGTT TGGCCGCAAC TGTCAGGAGA CGTGCCAGAG AGCCCAGGGC 
TGCAGGGGGC TGAGCTTCTG CCTGCCGGAC CCCTATGGCT GCTCCTGCGC CTCGGGGTGG 
AGCGGCTCCC GCTGCAGCCA AGCCTGTCCC CCAGGATATT ATGGCCCCGA CTGCGCTCTG 
ATGTGTGCCT GCCGGAATGG TGGCAGCTGT AACCGCTTCA GTGGCTGTGT CTGCCCTGCA 
GGCTGGCATG GGCAGCACTG CGAGAAGTCA GACCGCTTCC CCCAGATCAT CAAACTGGCC 
TCGGAGCTGG AGTTCAACCT GGGCTCAGAG CCCATCATCA GCTGCGTGGC CACTGGCAAC 
CCACTGCCTG CAAGTGATGG TGTGGAGCTG CGCAAGGCTG ATGGCACCAT GCTCAAGCTG 
GTCAAAGCCA TCATAGAGTC GGGGCAGATC ACGTGCGAGT TTCAGGTGCA GCATCTGACG 
AAGGCCGACG CAGGGCTCTG GGAGTGCCGA GTCTCTACTA CTGGGGGCCA GGACAGCCGG 
AAAGTCAAGG TCAACATTCG AGTGCCACCA GCACCCCTGA GCCCCCCCCG GCTGCTGGCC 
AAGCAGAGTC GCCAGCTTGT GGTGTCACCT GTGGACTGCT TCTCTGGTGA TGGGCCCATT 
GTCTCTGTCA AGTTACTCTA CAAGCCCAAG GATGATGCCT CGGTGTGGTC GTCCATTGTG 
GTTGACAACA GTGAAAACAT CACCCTCATG AACCTCCGGC CGGTGACCGC CTATGTTGTC 
AAGGTACAGC TGAGCCGACC AGGGGATGGT GGTGAGGGCA GCAAGGGACC TGAGGCCGTC 
ATGGTGACTG AGTGCCTAGA GCCCACAGTC AAGCCTGTGA TTGAAGGTTG GTCCATTGAG 
GAGAAGAACA CCCTTCATGT CAACTGGAAA TTGCCGAGTA ACCATGAGCC AGCACATGGA 
TTCATCGTCC ATCTCTTCGA CTCCGCAAAG CAACTGGTCT ACAAGAAAAA CATCACGTCC 
ATTTCCGTAC TCTCTGCCCA CATTGGGGGC CTGGAGTTCA ACAAGGAGTA TGGGCTGGAG 
GTGCTGGTGT ACCACTGCAC CAGCCTGGGG CCCCCCTCTG ACCTCTACAA GGTCATGATC 
AACAGCAAAG GGCCTTCCTC CCCACGGTCA CTCTCAGCAG AGTCTGTGTC TGACACAGCC 
ATCAGGCTCT ACTGGCAGGT CCCCGAGTAC CCCAACGGGG GCATCACCAA GTACATTGTG 
GAGCTGCAGC AGGTGGGGGG GACCAGTGAG CCCCAGTGGA TTGACACCGA CAATGGCGCC 
GAGACCACCA AGATCATTGG AGGCCTCAAC GCCAGCACCA CCTACCAGTT TCGTGTCCGG 
GCCAACTCCC ATGTCCCAGG GGAGTGGAGC CAGCTGGTGA AAGCCAAAAC CCTGGGGGAT 
GGAGCACTGA GTGTGCCACC CAGCCTGGGC AGCCAGAGCA CCGAGCAGGC AGGAGTAGAC 
CAGCAGCTGC TCTTGGCCAT CATCGGCTCC GTGTCCGTCA CCTGCCTCAC CATCCTCTTT 
GCCCTCCTGG CACTTTTTCT CATCAAGAAG AACTTTTTCC ACCGGCGCCG CACCTTCACA 
TACCAGTCTG GCTCGGTGAG TGCTGCCTGC CCTGCCAGGA CAGTGTCCCC AGCTCCCCAC 
GTCCCAGCCC GATCCAGGTC ACGCTCCATA GCCTCCAGTC CGGATGCAGC CAGACCTCAC 
CTCCACACAG AGAGGGGAGA AGAGACCATC CTGCAGTTCA ATTCAGGGAC CCTGACCCTG 
ACGCGCCGGC CCAAGCCACA GCCCGAACCC CTCAGCTACC CCATCCTGGA GTGGGAGGAC 
ATCAAGTTTG AGGACATGAT TGGGGAAGGG AACTTTGGGC AGGTCATCAG GGCCATGATC 
AAAAAAGATG GCTTGAAAAT GAATGCAGCC ATCAAGATGC TGAAGGAGTT CGCCTCAGAG 
AATGACCACC GGGACTTTGC TGGAGAGTTA GAGGTGCTGT GCAAGCTGGG CCATCACCCC 
AACATAATCA ACTTGCTAGG CGCCTGTGAG AACAAGGGTT ACCTGTATAT TGCCATCGAG 
TATGCCCCCT ATGGAAACCT CCTCGACTTC CTCCGCAAAA GCCGAGTCCT GGAGACCGAC 
CCAGCCTTTG CCAAGGAGCA TGGCACCGCG TCCACCCTCA CCTCCCAGCA GCTCCTCCAG 
TTCGCTTCAG ACGTGGCCAA GGGGATGCAG TACCTAAGTG AGAAGCAGTT CATTCACAGG 
GACCTGGCAG CCAGGAATAT CCTGGTGGGA GAAAACCTGG CCTCCAAGAT CGCTGACTTT 
GGCCTTTCCA GGGGGGAGGA GGTCTACGTG AAGAAGACAA TGGGCCGCTT GCCAGTTCGT 
TGGATGGCCA TCGAGTCCCT CAACTACAGC GTGTACACCA CCAAGAGCGA TGTGTGGTCC 
TTTGGCGTCC TTCTCTGGGA GATCGTCAGC TTGGGGGGCA CGCCGTACTG CGGGATGACG 
TGTGCTGAGC TCTACGAGAA GCTGCCCCAG GGCTATCGCA TGGAGAAACC CCGCAACTGT 
GATGATGAGG TGTACGAGCT GATGAGGCAG TGCTGGCGCG ACCGTCCCTA CGAGCGCCCA 
CCCTTTGCCC AGATCTCCAT GCAGCTCATC CGCATGCTGG AGGCCAGGAA GGCATACGTG 
AACATGGCCC TGTTTGAGAA CTTCACCTAC GCAGGGATTG ATGCCACCGC GGAGGAGGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015146806.1
CDS	464..4006
Translation	MCWIFSCARFGTHFKDMGLQFYLLLLLPWMAGAILDITLIANVQSLSHSDFFLSCIMGERDVSYLQIERENKIVMTHPKTGFQNYRNRSNYVQARGFSMPDLVGILYCLGRTPTEQAQVVYVHNSHNAHLFPVKATQSVNVGEPATFSARVLRRKEMDVMWKRNGTYYQTTDRGEVRGDLFTLTLPNVSVTENGVYSATFMGDSPLWSAFYRLIVRACAAKKWGPSCEKDCPDCLNGGICHDHVGECICPPGFTGTRCERACREGQFGRNCQETCQRAQGCRGLSFCLPDPYGCSCASGWSGSRCSQACPPGYYGPDCALMCACRNGGSCNRFSGCVCPAGWHGQHCEKSDRFPQIIKLASELEFNLGSEPIISCVATGNPLPASDGVELRKADGTMLKLVKAIIESGQITCEFQVQHLTKADAGLWECRVSTTGGQDSRKVKVNIRVPPAPLSPPRLLAKQSRQLVVSPVDCFSGDGPIVSVKLLYKPKDDASVWSSIVVDNSENITLMNLRPVTAYVVKVQLSRPGDGGEGSKGPEAVMVTECLEPTVKPVIEGWSIEEKNTLHVNWKLPSNHEPAHGFIVHLFDSAKQLVYKKNITSISVLSAHIGGLEFNKEYGLEVLVYHCTSLGPPSDLYKVMINSKGPSSPRSLSAESVSDTAIRLYWQVPEYPNGGITKYIVELQQVGGTSEPQWIDTDNGAETTKIIGGLNASTTYQFRVRANSHVPGEWSQLVKAKTLGDGALSVPPSLGSQSTEQAGVDQQLLLAIIGSVSVTCLTILFALLALFLIKKNFFHRRRTFTYQSGSVSAACPARTVSPAPHVPARSRSRSIASSPDAARPHLHTERGEETILQFNSGTLTLTRRPKPQPEPLSYPILEWEDIKFEDMIGEGNFGQVIRAMIKKDGLKMNAAIKMLKEFASENDHRDFAGELEVLCKLGHHPNIINLLGACENKGYLYIAIEYAPYGNLLDFLRKSRVLETDPAFAKEHGTASTLTSQQLLQFASDVAKGMQYLSEKQFIHRDLAARNILVGENLASKIADFGLSRGEEVYVKKTMGRLPVRWMAIESLNYSVYTTKSDVWSFGVLLWEIVSLGGTPYCGMTCAELYEKLPQGYRMEKPRNCDDEVYELMRQCWRDRPYERPPFAQISMQLIRMLEARKAYVNMALFENFTYAGIDATAEEA

Target ORF information:

RefSeq Version	XM_015291320.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291320.2

ORF Insert Sequence:

ATGTGCTGGA TATTTTCCTG TGCCAGATTT GGAACACACT TCAAGGACAT GGGACTCCAG 
TTTTATCTAC TGCTTCTCCT CCCATGGATG GCAGGGGCCA TCCTAGACAT CACCCTGATT 
GCCAACGTGC AGAGCCTGTC CCACTCTGAC TTCTTCCTCT CCTGCATCAT GGGCGAGCGT 
GACGTGAGCT ACCTGCAGAT TGAGAGGGAG AACAAGATTG TGATGACGCA CCCCAAGACA 
GGCTTCCAAA ACTACCGCAA CCGCAGCAAC TACGTCCAGG CCAGGGGCTT CTCCATGCCT 
GACCTGGTGG GCATCCTCTA CTGCCTGGGG CGCACGCCAA CAGAGCAGGC CCAGGTGGTC 
TACGTGCACA ACAGCCACAA TGCCCACCTC TTCCCAGTGA AGGCCACGCA GTCTGTGAAT 
GTCGGCGAGC CGGCCACCTT CTCCGCCAGG GTCCTCAGGA GGAAGGAGAT GGATGTCATG 
TGGAAAAGAA ATGGTACCTA CTACCAGACC ACGGACCGGG GCGAGGTGCG GGGTGACCTT 
TTCACTCTAA CACTCCCGAA TGTCAGCGTA ACTGAAAATG GTGTCTACAG TGCCACTTTC 
ATGGGAGATA GCCCTCTGTG GAGTGCCTTC TACAGGCTAA TCGTCAGAGC GTGTGCTGCA 
AAGAAATGGG GTCCGTCCTG TGAGAAGGAC TGTCCTGACT GCCTGAATGG GGGCATCTGT 
CATGACCACG TCGGTGAATG CATCTGTCCT CCTGGCTTCA CAGGCACCCG TTGTGAGAGA 
GCCTGCCGGG AAGGCCAGTT TGGCCGCAAC TGTCAGGAGA CGTGCCAGAG AGCCCAGGGC 
TGCAGGGGGC TGAGCTTCTG CCTGCCGGAC CCCTATGGCT GCTCCTGCGC CTCGGGGTGG 
AGCGGCTCCC GCTGCAGCCA AGCCTGTCCC CCAGGATATT ATGGCCCCGA CTGCGCTCTG 
ATGTGTGCCT GCCGGAATGG TGGCAGCTGT AACCGCTTCA GTGGCTGTGT CTGCCCTGCA 
GGCTGGCATG GGCAGCACTG CGAGAAGTCA GACCGCTTCC CCCAGATCAT CAAACTGGCC 
TCGGAGCTGG AGTTCAACCT GGGCTCAGAG CCCATCATCA GCTGCGTGGC CACTGGCAAC 
CCACTGCCTG CAAGTGATGG TGTGGAGCTG CGCAAGGCTG ATGGCACCAT GCTCAAGCTG 
GTCAAAGCCA TCATAGAGTC GGGGCAGATC ACGTGCGAGT TTCAGGTGCA GCATCTGACG 
AAGGCCGACG CAGGGCTCTG GGAGTGCCGA GTCTCTACTA CTGGGGGCCA GGACAGCCGG 
AAAGTCAAGG TCAACATTCG AGTGCCACCA GCACCCCTGA GCCCCCCCCG GCTGCTGGCC 
AAGCAGAGTC GCCAGCTTGT GGTGTCACCT GTGGACTGCT TCTCTGGTGA TGGGCCCATT 
GTCTCTGTCA AGTTACTCTA CAAGCCCAAG GATGATGCCT CGGTGTGGTC GTCCATTGTG 
GTTGACAACA GTGAAAACAT CACCCTCATG AACCTCCGGC CGGTGACCGC CTATGTTGTC 
AAGGTACAGC TGAGCCGACC AGGGGATGGT GGTGAGGGCA GCAAGGGACC TGAGGCCGTC 
ATGGTGACTG AGTGCCTAGA GCCCACAGTC AAGCCTGTGA TTGAAGGTTG GTCCATTGAG 
GAGAAGAACA CCCTTCATGT CAACTGGAAA TTGCCGAGTA ACCATGAGCC AGCACATGGA 
TTCATCGTCC ATCTCTTCGA CTCCGCAAAG CAACTGGTCT ACAAGAAAAA CATCACGTCC 
ATTTCCGTAC TCTCTGCCCA CATTGGGGGC CTGGAGTTCA ACAAGGAGTA TGGGCTGGAG 
GTGCTGGTGT ACCACTGCAC CAGCCTGGGG CCCCCCTCTG ACCTCTACAA GGTCATGATC 
AACAGCAAAG GGCCTTCCTC CCCACGGTCA CTCTCAGCAG AGTCTGTGTC TGACACAGCC 
ATCAGGCTCT ACTGGCAGGT CCCCGAGTAC CCCAACGGGG GCATCACCAA GTACATTGTG 
GAGCTGCAGC AGGTGGGGGG GACCAGTGAG CCCCAGTGGA TTGACACCGA CAATGGCGCC 
GAGACCACCA AGATCATTGG AGGCCTCAAC GCCAGCACCA CCTACCAGTT TCGTGTCCGG 
GCCAACTCCC ATGTCCCAGG GGAGTGGAGC CAGCTGGTGA AAGCCAAAAC CCTGGGGGAT 
GGAGCACTGA GTGTGCCACC CAGCCTGGGC AGCCAGAGCA CCGAGCAGGC AGGAGTAGAC 
CAGCAGCTGC TCTTGGCCAT CATCGGCTCC GTGTCCGTCA CCTGCCTCAC CATCCTCTTT 
GCCCTCCTGG CACTTTTTCT CATCAAGAAG AACTTTTTCC ACCGGCGCCG CACCTTCACA 
TACCAGTCTG GCTCGGTGAG TGCTGCCTGC CCTGCCAGGA CAGTGTCCCC AGCTCCCCAC 
GTCCCAGCCC GATCCAGGTC ACGCTCCATA GCCTCCAGTC CGGATGCAGC CAGACCTCAC 
CTCCACACAG AGAGGGGAGA AGAGACCATC CTGCAGTTCA ATTCAGGGAC CCTGACCCTG 
ACGCGCCGGC CCAAGCCACA GCCCGAACCC CTCAGCTACC CCATCCTGGA GTGGGAGGAC 
ATCAAGTTTG AGGACATGAT TGGGGAAGGG AACTTTGGGC AGGTCATCAG GGCCATGATC 
AAAAAAGATG GCTTGAAAAT GAATGCAGCC ATCAAGATGC TGAAGGAGTT CGCCTCAGAG 
AATGACCACC GGGACTTTGC TGGAGAGTTA GAGGTGCTGT GCAAGCTGGG CCATCACCCC 
AACATAATCA ACTTGCTAGG CGCCTGTGAG AACAAGGGTT ACCTGTATAT TGCCATCGAG 
TATGCCCCCT ATGGAAACCT CCTCGACTTC CTCCGCAAAA GCCGAGTCCT GGAGACCGAC 
CCAGCCTTTG CCAAGGAGCA TGGCACCGCG TCCACCCTCA CCTCCCAGCA GCTCCTCCAG 
TTCGCTTCAG ACGTGGCCAA GGGGATGCAG TACCTAAGTG AGAAGCAGTT CATTCACAGG 
GACCTGGCAG CCAGGAATAT CCTGGTGGGA GAAAACCTGG CCTCCAAGAT CGCTGACTTT 
GGCCTTTCCA GGGGGGAGGA GGTCTACGTG AAGAAGACAA TGGGCCGCTT GCCAGTTCGT 
TGGATGGCCA TCGAGTCCCT CAACTACAGC GTGTACACCA CCAAGAGCGA TGTGTGGTCC 
TTTGGCGTCC TTCTCTGGGA GATCGTCAGC TTGGGGGGCA CGCCGTACTG CGGGATGACG 
TGTGCTGAGC TCTACGAGAA GCTGCCCCAG GGCTATCGCA TGGAGAAACC CCGCAACTGT 
GATGATGAGG TGTACGAGCT GATGAGGCAG TGCTGGCGCG ACCGTCCCTA CGAGCGCCCA 
CCCTTTGCCC AGATCTCCAT GCAGCTCATC CGCATGCTGG AGGCCAGGAA GGCATACGTG 
AACATGGCCC TGTTTGAGAA CTTCACCTAC GCAGGGATTG ATGCCACCGC GGAGGAGGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIE1 ( XM_422400.5 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_422400.4 Gallus gallus tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (TIE1), transcript variant X2, mRNA.

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CloneID	OGa12010
Clone ID Related Accession (Same CDS sequence)	XM_422400.5 , XM_422400.6
Accession Version	XM_422400.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	tyrosine-protein kinase receptor Tie-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455913.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_422400.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCTGGA TATTTTCCTG TGCCAGATTT GGAACACACT TCAAGGACAT GGGACTCCAG 
TTTTATCTAC TGCTTCTCCT CCCATGGATG GCAGGGGCCA TCCTAGACAT CACCCTGATT 
GCCAACGTGC AGAGCCTGTC CCACTCTGAC TTCTTCCTCT CCTGCATCAT GGGCGAGCGT 
GACGTGAGCT ACCTGCAGAT TGAGAGGGAG AACAAGATTG TGATGACGCA CCCCAAGACA 
GGCTTCCAAA ACTACCGCAA CCGCAGCAAC TACGTCCAGG CCAGGGGCTT CTCCATGCCT 
GACCTGGTGG GCATCCTCTA CTGCCTGGGG CGCACGCCAA CAGAGCAGGC CCAGGTGGTC 
TACGTGCACA ACAGCCACAA TGCCCACCTC TTCCCAGTGA AGGCCACGCA GTCTGTGAAT 
GTCGGCGAGC CGGCCACCTT CTCCGCCAGG GTCCTCAGGA GGAAGGAGAT GGATGTCATG 
TGGAAAAGAA ATGGTACCTA CTACCAGACC ACGGACCGGG GCGAGGTGCG GGGTGACCTT 
TTCACTCTAA CACTCCCGAA TGTCAGCGTA ACTGAAAATG GTGTCTACAG TGCCACTTTC 
ATGGGAGATA GCCCTCTGTG GAGTGCCTTC TACAGGCTAA TCGTCAGAGC GTGTGCTGCA 
AAGAAATGGG GTCCGTCCTG TGAGAAGGAC TGTCCTGACT GCCTGAATGG GGGCATCTGT 
CATGACCACG TCGGTGAATG CATCTGTCCT CCTGGCTTCA CAGGCACCCG TTGTGAGAGA 
GCCTGCCGGG AAGGCCAGTT TGGCCGCAAC TGTCAGGAGA CGTGCCAGAG AGCCCAGGGC 
TGCAGGGGGC TGAGCTTCTG CCTGCCGGAC CCCTATGGCT GCTCCTGCGC CTCGGGGTGG 
AGCGGCTCCC GCTGCAGCCA AGCCTGTCCC CCAGGATATT ATGGCCCCGA CTGCGCTCTG 
ATGTGTGCCT GCCGGAATGG TGGCAGCTGT AACCGCTTCA GTGGCTGTGT CTGCCCTGCA 
GGCTGGCATG GGCAGCACTG CGAGAAGTCA GACCGCTTCC CCCAGATCAT CAAACTGGCC 
TCGGAGCTGG AGTTCAACCT GGGCTCAGAG CCCATCATCA GCTGCGTGGC CACTGGCAAC 
CCACTGCCTG CAAGTGATGG TGTGGAGCTG CGCAAGGCTG ATGGCACCAT GCTCAAGCTG 
GTCAAAGCCA TCATAGAGTC GGGGCAGATC ACGTGCGAGT TTCAGGTGCA GCATCTGACG 
AAGGCCGACG CAGGGCTCTG GGAGTGCCGA GTCTCTACTA CTGGGGGCCA GGACAGCCGG 
AAAGTCAAGG TCAACATTCG AGTGCCACCA GCACCCCTGA GCCCCCCCCG GCTGCTGGCC 
AAGCAGAGTC GCCAGCTTGT GGTGTCACCT GTGGACTGCT TCTCTGGTGA TGGGCCCATT 
GTCTCTGTCA AGTTACTCTA CAAGCCCAAG GATGATGCCT CGGTGTGGTC GTCCATTGTG 
GTTGACAACA GTGAAAACAT CACCCTCATG AACCTCCGGC CGGTGACCGC CTATGTTGTC 
AAGGTACAGC TGAGCCGACC AGGGGATGGT GGTGAGGGCA GCAAGGGACC TGAGGCCGTC 
ATGGTGACTG AGTGCCTAGA GCCCACAGTC AAGCCTGTGA TTGAAGGTTG GTCCATTGAG 
GAGAAGAACA CCCTTCATGT CAACTGGAAA TTGCCGAGTA ACCATGAGCC AGCACATGGA 
TTCATCGTCC ATCTCTTCGA CTCCGCAAAG CAACTGGTCT ACAAGAAAAA CATCACGTCC 
ATTTCCGTAC TCTCTGCCCA CATTGGGGGC CTGGAGTTCA ACAAGGAGTA TGGGCTGGAG 
GTGCTGGTGT ACCACTGCAC CAGCCTGGGG CCCCCCTCTG ACCTCTACAA GGTCATGATC 
AACAGCAAAG GGCCTTCCTC CCCACGGTCA CTCTCAGCAG AGTCTGTGTC TGACACAGCC 
ATCAGGCTCT ACTGGCAGGT CCCCGAGTAC CCCAACGGGG GCATCACCAA GTACATTGTG 
GAGCTGCAGC AGGTGGGGGG GACCAGTGAG CCCCAGTGGA TTGACACCGA CAATGGCGCC 
GAGACCACCA AGATCATTGG AGGCCTCAAC GCCAGCACCA CCTACCAGTT TCGTGTCCGG 
GCCAACTCCC ATGTCCCAGG GGAGTGGAGC CAGCTGGTGA AAGCCAAAAC CCTGGGGGAT 
GGAGCACTGA GTGTGCCACC CAGCCTGGGC AGCCAGAGCA CCGAGCAGGC AGGAGTAGAC 
CAGCAGCTGC TCTTGGCCAT CATCGGCTCC GTGTCCGTCA CCTGCCTCAC CATCCTCTTT 
GCCCTCCTGG CACTTTTTCT CATCAAGAAG AACTTTTTCC ACCGGCGCCG CACCTTCACA 
TACCAGTCTG GCTCGGGAGA AGAGACCATC CTGCAGTTCA ATTCAGGGAC CCTGACCCTG 
ACGCGCCGGC CCAAGCCACA GCCCGAACCC CTCAGCTACC CCATCCTGGA GTGGGAGGAC 
ATCAAGTTTG AGGACATGAT TGGGGAAGGG AACTTTGGGC AGGTCATCAG GGCCATGATC 
AAAAAAGATG GCTTGAAAAT GAATGCAGCC ATCAAGATGC TGAAGGAGTT CGCCTCAGAG 
AATGACCACC GGGACTTTGC TGGAGAGTTA GAGGTGCTGT GCAAGCTGGG CCATCACCCC 
AACATAATCA ACTTGCTAGG CGCCTGTGAG AACAAGGGTT ACCTGTATAT TGCCATCGAG 
TATGCCCCCT ATGGAAACCT CCTCGACTTC CTCCGCAAAA GCCGAGTCCT GGAGACCGAC 
CCAGCCTTTG CCAAGGAGCA TGGCACCGCG TCCACCCTCA CCTCCCAGCA GCTCCTCCAG 
TTCGCTTCAG ACGTGGCCAA GGGGATGCAG TACCTAAGTG AGAAGCAGTT CATTCACAGG 
GACCTGGCAG CCAGGAATAT CCTGGTGGGA GAAAACCTGG CCTCCAAGAT CGCTGACTTT 
GGCCTTTCCA GGGGGGAGGA GGTCTACGTG AAGAAGACAA TGGGCCGCTT GCCAGTTCGT 
TGGATGGCCA TCGAGTCCCT CAACTACAGC GTGTACACCA CCAAGAGCGA TGTGTGGTCC 
TTTGGCGTCC TTCTCTGGGA GATCGTCAGC TTGGGGGGCA CGCCGTACTG CGGGATGACG 
TGTGCTGAGC TCTACGAGAA GCTGCCCCAG GGCTATCGCA TGGAGAAACC CCGCAACTGT 
GATGATGAGG TGTACGAGCT GATGAGGCAG TGCTGGCGCG ACCGTCCCTA CGAGCGCCCA 
CCCTTTGCCC AGATCTCCAT GCAGCTCATC CGCATGCTGG AGGCCAGGAA GGCATACGTG 
AACATGGCCC TGTTTGAGAA CTTCACCTAC GCAGGGATTG ATGCCACCGC GGAGGAGGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_422400.4
CDS	1362..4784
Translation	MCWIFSCARFGTHFKDMGLQFYLLLLLPWMAGAILDITLIANVQSLSHSDFFLSCIMGERDVSYLQIERENKIVMTHPKTGFQNYRNRSNYVQARGFSMPDLVGILYCLGRTPTEQAQVVYVHNSHNAHLFPVKATQSVNVGEPATFSARVLRRKEMDVMWKRNGTYYQTTDRGEVRGDLFTLTLPNVSVTENGVYSATFMGDSPLWSAFYRLIVRACAAKKWGPSCEKDCPDCLNGGICHDHVGECICPPGFTGTRCERACREGQFGRNCQETCQRAQGCRGLSFCLPDPYGCSCASGWSGSRCSQACPPGYYGPDCALMCACRNGGSCNRFSGCVCPAGWHGQHCEKSDRFPQIIKLASELEFNLGSEPIISCVATGNPLPASDGVELRKADGTMLKLVKAIIESGQITCEFQVQHLTKADAGLWECRVSTTGGQDSRKVKVNIRVPPAPLSPPRLLAKQSRQLVVSPVDCFSGDGPIVSVKLLYKPKDDASVWSSIVVDNSENITLMNLRPVTAYVVKVQLSRPGDGGEGSKGPEAVMVTECLEPTVKPVIEGWSIEEKNTLHVNWKLPSNHEPAHGFIVHLFDSAKQLVYKKNITSISVLSAHIGGLEFNKEYGLEVLVYHCTSLGPPSDLYKVMINSKGPSSPRSLSAESVSDTAIRLYWQVPEYPNGGITKYIVELQQVGGTSEPQWIDTDNGAETTKIIGGLNASTTYQFRVRANSHVPGEWSQLVKAKTLGDGALSVPPSLGSQSTEQAGVDQQLLLAIIGSVSVTCLTILFALLALFLIKKNFFHRRRTFTYQSGSGEETILQFNSGTLTLTRRPKPQPEPLSYPILEWEDIKFEDMIGEGNFGQVIRAMIKKDGLKMNAAIKMLKEFASENDHRDFAGELEVLCKLGHHPNIINLLGACENKGYLYIAIEYAPYGNLLDFLRKSRVLETDPAFAKEHGTASTLTSQQLLQFASDVAKGMQYLSEKQFIHRDLAARNILVGENLASKIADFGLSRGEEVYVKKTMGRLPVRWMAIESLNYSVYTTKSDVWSFGVLLWEIVSLGGTPYCGMTCAELYEKLPQGYRMEKPRNCDDEVYELMRQCWRDRPYERPPFAQISMQLIRMLEARKAYVNMALFENFTYAGIDATAEEA

Target ORF information:

RefSeq Version	XM_422400.5
Organism	Gallus gallus(chicken)
Definition	Gallus gallus tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (TIE1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_422400.5

ORF Insert Sequence:

ATGTGCTGGA TATTTTCCTG TGCCAGATTT GGAACACACT TCAAGGACAT GGGACTCCAG 
TTTTATCTAC TGCTTCTCCT CCCATGGATG GCAGGGGCCA TCCTAGACAT CACCCTGATT 
GCCAACGTGC AGAGCCTGTC CCACTCTGAC TTCTTCCTCT CCTGCATCAT GGGCGAGCGT 
GACGTGAGCT ACCTGCAGAT TGAGAGGGAG AACAAGATTG TGATGACGCA CCCCAAGACA 
GGCTTCCAAA ACTACCGCAA CCGCAGCAAC TACGTCCAGG CCAGGGGCTT CTCCATGCCT 
GACCTGGTGG GCATCCTCTA CTGCCTGGGG CGCACGCCAA CAGAGCAGGC CCAGGTGGTC 
TACGTGCACA ACAGCCACAA TGCCCACCTC TTCCCAGTGA AGGCCACGCA GTCTGTGAAT 
GTCGGCGAGC CGGCCACCTT CTCCGCCAGG GTCCTCAGGA GGAAGGAGAT GGATGTCATG 
TGGAAAAGAA ATGGTACCTA CTACCAGACC ACGGACCGGG GCGAGGTGCG GGGTGACCTT 
TTCACTCTAA CACTCCCGAA TGTCAGCGTA ACTGAAAATG GTGTCTACAG TGCCACTTTC 
ATGGGAGATA GCCCTCTGTG GAGTGCCTTC TACAGGCTAA TCGTCAGAGC GTGTGCTGCA 
AAGAAATGGG GTCCGTCCTG TGAGAAGGAC TGTCCTGACT GCCTGAATGG GGGCATCTGT 
CATGACCACG TCGGTGAATG CATCTGTCCT CCTGGCTTCA CAGGCACCCG TTGTGAGAGA 
GCCTGCCGGG AAGGCCAGTT TGGCCGCAAC TGTCAGGAGA CGTGCCAGAG AGCCCAGGGC 
TGCAGGGGGC TGAGCTTCTG CCTGCCGGAC CCCTATGGCT GCTCCTGCGC CTCGGGGTGG 
AGCGGCTCCC GCTGCAGCCA AGCCTGTCCC CCAGGATATT ATGGCCCCGA CTGCGCTCTG 
ATGTGTGCCT GCCGGAATGG TGGCAGCTGT AACCGCTTCA GTGGCTGTGT CTGCCCTGCA 
GGCTGGCATG GGCAGCACTG CGAGAAGTCA GACCGCTTCC CCCAGATCAT CAAACTGGCC 
TCGGAGCTGG AGTTCAACCT GGGCTCAGAG CCCATCATCA GCTGCGTGGC CACTGGCAAC 
CCACTGCCTG CAAGTGATGG TGTGGAGCTG CGCAAGGCTG ATGGCACCAT GCTCAAGCTG 
GTCAAAGCCA TCATAGAGTC GGGGCAGATC ACGTGCGAGT TTCAGGTGCA GCATCTGACG 
AAGGCCGACG CAGGGCTCTG GGAGTGCCGA GTCTCTACTA CTGGGGGCCA GGACAGCCGG 
AAAGTCAAGG TCAACATTCG AGTGCCACCA GCACCCCTGA GCCCCCCCCG GCTGCTGGCC 
AAGCAGAGTC GCCAGCTTGT GGTGTCACCT GTGGACTGCT TCTCTGGTGA TGGGCCCATT 
GTCTCTGTCA AGTTACTCTA CAAGCCCAAG GATGATGCCT CGGTGTGGTC GTCCATTGTG 
GTTGACAACA GTGAAAACAT CACCCTCATG AACCTCCGGC CGGTGACCGC CTATGTTGTC 
AAGGTACAGC TGAGCCGACC AGGGGATGGT GGTGAGGGCA GCAAGGGACC TGAGGCCGTC 
ATGGTGACTG AGTGCCTAGA GCCCACAGTC AAGCCTGTGA TTGAAGGTTG GTCCATTGAG 
GAGAAGAACA CCCTTCATGT CAACTGGAAA TTGCCGAGTA ACCATGAGCC AGCACATGGA 
TTCATCGTCC ATCTCTTCGA CTCCGCAAAG CAACTGGTCT ACAAGAAAAA CATCACGTCC 
ATTTCCGTAC TCTCTGCCCA CATTGGGGGC CTGGAGTTCA ACAAGGAGTA TGGGCTGGAG 
GTGCTGGTGT ACCACTGCAC CAGCCTGGGG CCCCCCTCTG ACCTCTACAA GGTCATGATC 
AACAGCAAAG GGCCTTCCTC CCCACGGTCA CTCTCAGCAG AGTCTGTGTC TGACACAGCC 
ATCAGGCTCT ACTGGCAGGT CCCCGAGTAC CCCAACGGGG GCATCACCAA GTACATTGTG 
GAGCTGCAGC AGGTGGGGGG GACCAGTGAG CCCCAGTGGA TTGACACCGA CAATGGCGCC 
GAGACCACCA AGATCATTGG AGGCCTCAAC GCCAGCACCA CCTACCAGTT TCGTGTCCGG 
GCCAACTCCC ATGTCCCAGG GGAGTGGAGC CAGCTGGTGA AAGCCAAAAC CCTGGGGGAT 
GGAGCACTGA GTGTGCCACC CAGCCTGGGC AGCCAGAGCA CCGAGCAGGC AGGAGTAGAC 
CAGCAGCTGC TCTTGGCCAT CATCGGCTCC GTGTCCGTCA CCTGCCTCAC CATCCTCTTT 
GCCCTCCTGG CACTTTTTCT CATCAAGAAG AACTTTTTCC ACCGGCGCCG CACCTTCACA 
TACCAGTCTG GCTCGGGAGA AGAGACCATC CTGCAGTTCA ATTCAGGGAC CCTGACCCTG 
ACGCGCCGGC CCAAGCCACA GCCCGAACCC CTCAGCTACC CCATCCTGGA GTGGGAGGAC 
ATCAAGTTTG AGGACATGAT TGGGGAAGGG AACTTTGGGC AGGTCATCAG GGCCATGATC 
AAAAAAGATG GCTTGAAAAT GAATGCAGCC ATCAAGATGC TGAAGGAGTT CGCCTCAGAG 
AATGACCACC GGGACTTTGC TGGAGAGTTA GAGGTGCTGT GCAAGCTGGG CCATCACCCC 
AACATAATCA ACTTGCTAGG CGCCTGTGAG AACAAGGGTT ACCTGTATAT TGCCATCGAG 
TATGCCCCCT ATGGAAACCT CCTCGACTTC CTCCGCAAAA GCCGAGTCCT GGAGACCGAC 
CCAGCCTTTG CCAAGGAGCA TGGCACCGCG TCCACCCTCA CCTCCCAGCA GCTCCTCCAG 
TTCGCTTCAG ACGTGGCCAA GGGGATGCAG TACCTAAGTG AGAAGCAGTT CATTCACAGG 
GACCTGGCAG CCAGGAATAT CCTGGTGGGA GAAAACCTGG CCTCCAAGAT CGCTGACTTT 
GGCCTTTCCA GGGGGGAGGA GGTCTACGTG AAGAAGACAA TGGGCCGCTT GCCAGTTCGT 
TGGATGGCCA TCGAGTCCCT CAACTACAGC GTGTACACCA CCAAGAGCGA TGTGTGGTCC 
TTTGGCGTCC TTCTCTGGGA GATCGTCAGC TTGGGGGGCA CGCCGTACTG CGGGATGACG 
TGTGCTGAGC TCTACGAGAA GCTGCCCCAG GGCTATCGCA TGGAGAAACC CCGCAACTGT 
GATGATGAGG TGTACGAGCT GATGAGGCAG TGCTGGCGCG ACCGTCCCTA CGAGCGCCCA 
CCCTTTGCCC AGATCTCCAT GCAGCTCATC CGCATGCTGG AGGCCAGGAA GGCATACGTG 
AACATGGCCC TGTTTGAGAA CTTCACCTAC GCAGGGATTG ATGCCACCGC GGAGGAGGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIE1 ( XM_422400.6 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_422400.4 Gallus gallus tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa12010
Clone ID Related Accession (Same CDS sequence)	XM_422400.5 , XM_422400.6
Accession Version	XM_422400.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	tyrosine-protein kinase receptor Tie-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_422400.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCTGGA TATTTTCCTG TGCCAGATTT GGAACACACT TCAAGGACAT GGGACTCCAG 
TTTTATCTAC TGCTTCTCCT CCCATGGATG GCAGGGGCCA TCCTAGACAT CACCCTGATT 
GCCAACGTGC AGAGCCTGTC CCACTCTGAC TTCTTCCTCT CCTGCATCAT GGGCGAGCGT 
GACGTGAGCT ACCTGCAGAT TGAGAGGGAG AACAAGATTG TGATGACGCA CCCCAAGACA 
GGCTTCCAAA ACTACCGCAA CCGCAGCAAC TACGTCCAGG CCAGGGGCTT CTCCATGCCT 
GACCTGGTGG GCATCCTCTA CTGCCTGGGG CGCACGCCAA CAGAGCAGGC CCAGGTGGTC 
TACGTGCACA ACAGCCACAA TGCCCACCTC TTCCCAGTGA AGGCCACGCA GTCTGTGAAT 
GTCGGCGAGC CGGCCACCTT CTCCGCCAGG GTCCTCAGGA GGAAGGAGAT GGATGTCATG 
TGGAAAAGAA ATGGTACCTA CTACCAGACC ACGGACCGGG GCGAGGTGCG GGGTGACCTT 
TTCACTCTAA CACTCCCGAA TGTCAGCGTA ACTGAAAATG GTGTCTACAG TGCCACTTTC 
ATGGGAGATA GCCCTCTGTG GAGTGCCTTC TACAGGCTAA TCGTCAGAGC GTGTGCTGCA 
AAGAAATGGG GTCCGTCCTG TGAGAAGGAC TGTCCTGACT GCCTGAATGG GGGCATCTGT 
CATGACCACG TCGGTGAATG CATCTGTCCT CCTGGCTTCA CAGGCACCCG TTGTGAGAGA 
GCCTGCCGGG AAGGCCAGTT TGGCCGCAAC TGTCAGGAGA CGTGCCAGAG AGCCCAGGGC 
TGCAGGGGGC TGAGCTTCTG CCTGCCGGAC CCCTATGGCT GCTCCTGCGC CTCGGGGTGG 
AGCGGCTCCC GCTGCAGCCA AGCCTGTCCC CCAGGATATT ATGGCCCCGA CTGCGCTCTG 
ATGTGTGCCT GCCGGAATGG TGGCAGCTGT AACCGCTTCA GTGGCTGTGT CTGCCCTGCA 
GGCTGGCATG GGCAGCACTG CGAGAAGTCA GACCGCTTCC CCCAGATCAT CAAACTGGCC 
TCGGAGCTGG AGTTCAACCT GGGCTCAGAG CCCATCATCA GCTGCGTGGC CACTGGCAAC 
CCACTGCCTG CAAGTGATGG TGTGGAGCTG CGCAAGGCTG ATGGCACCAT GCTCAAGCTG 
GTCAAAGCCA TCATAGAGTC GGGGCAGATC ACGTGCGAGT TTCAGGTGCA GCATCTGACG 
AAGGCCGACG CAGGGCTCTG GGAGTGCCGA GTCTCTACTA CTGGGGGCCA GGACAGCCGG 
AAAGTCAAGG TCAACATTCG AGTGCCACCA GCACCCCTGA GCCCCCCCCG GCTGCTGGCC 
AAGCAGAGTC GCCAGCTTGT GGTGTCACCT GTGGACTGCT TCTCTGGTGA TGGGCCCATT 
GTCTCTGTCA AGTTACTCTA CAAGCCCAAG GATGATGCCT CGGTGTGGTC GTCCATTGTG 
GTTGACAACA GTGAAAACAT CACCCTCATG AACCTCCGGC CGGTGACCGC CTATGTTGTC 
AAGGTACAGC TGAGCCGACC AGGGGATGGT GGTGAGGGCA GCAAGGGACC TGAGGCCGTC 
ATGGTGACTG AGTGCCTAGA GCCCACAGTC AAGCCTGTGA TTGAAGGTTG GTCCATTGAG 
GAGAAGAACA CCCTTCATGT CAACTGGAAA TTGCCGAGTA ACCATGAGCC AGCACATGGA 
TTCATCGTCC ATCTCTTCGA CTCCGCAAAG CAACTGGTCT ACAAGAAAAA CATCACGTCC 
ATTTCCGTAC TCTCTGCCCA CATTGGGGGC CTGGAGTTCA ACAAGGAGTA TGGGCTGGAG 
GTGCTGGTGT ACCACTGCAC CAGCCTGGGG CCCCCCTCTG ACCTCTACAA GGTCATGATC 
AACAGCAAAG GGCCTTCCTC CCCACGGTCA CTCTCAGCAG AGTCTGTGTC TGACACAGCC 
ATCAGGCTCT ACTGGCAGGT CCCCGAGTAC CCCAACGGGG GCATCACCAA GTACATTGTG 
GAGCTGCAGC AGGTGGGGGG GACCAGTGAG CCCCAGTGGA TTGACACCGA CAATGGCGCC 
GAGACCACCA AGATCATTGG AGGCCTCAAC GCCAGCACCA CCTACCAGTT TCGTGTCCGG 
GCCAACTCCC ATGTCCCAGG GGAGTGGAGC CAGCTGGTGA AAGCCAAAAC CCTGGGGGAT 
GGAGCACTGA GTGTGCCACC CAGCCTGGGC AGCCAGAGCA CCGAGCAGGC AGGAGTAGAC 
CAGCAGCTGC TCTTGGCCAT CATCGGCTCC GTGTCCGTCA CCTGCCTCAC CATCCTCTTT 
GCCCTCCTGG CACTTTTTCT CATCAAGAAG AACTTTTTCC ACCGGCGCCG CACCTTCACA 
TACCAGTCTG GCTCGGGAGA AGAGACCATC CTGCAGTTCA ATTCAGGGAC CCTGACCCTG 
ACGCGCCGGC CCAAGCCACA GCCCGAACCC CTCAGCTACC CCATCCTGGA GTGGGAGGAC 
ATCAAGTTTG AGGACATGAT TGGGGAAGGG AACTTTGGGC AGGTCATCAG GGCCATGATC 
AAAAAAGATG GCTTGAAAAT GAATGCAGCC ATCAAGATGC TGAAGGAGTT CGCCTCAGAG 
AATGACCACC GGGACTTTGC TGGAGAGTTA GAGGTGCTGT GCAAGCTGGG CCATCACCCC 
AACATAATCA ACTTGCTAGG CGCCTGTGAG AACAAGGGTT ACCTGTATAT TGCCATCGAG 
TATGCCCCCT ATGGAAACCT CCTCGACTTC CTCCGCAAAA GCCGAGTCCT GGAGACCGAC 
CCAGCCTTTG CCAAGGAGCA TGGCACCGCG TCCACCCTCA CCTCCCAGCA GCTCCTCCAG 
TTCGCTTCAG ACGTGGCCAA GGGGATGCAG TACCTAAGTG AGAAGCAGTT CATTCACAGG 
GACCTGGCAG CCAGGAATAT CCTGGTGGGA GAAAACCTGG CCTCCAAGAT CGCTGACTTT 
GGCCTTTCCA GGGGGGAGGA GGTCTACGTG AAGAAGACAA TGGGCCGCTT GCCAGTTCGT 
TGGATGGCCA TCGAGTCCCT CAACTACAGC GTGTACACCA CCAAGAGCGA TGTGTGGTCC 
TTTGGCGTCC TTCTCTGGGA GATCGTCAGC TTGGGGGGCA CGCCGTACTG CGGGATGACG 
TGTGCTGAGC TCTACGAGAA GCTGCCCCAG GGCTATCGCA TGGAGAAACC CCGCAACTGT 
GATGATGAGG TGTACGAGCT GATGAGGCAG TGCTGGCGCG ACCGTCCCTA CGAGCGCCCA 
CCCTTTGCCC AGATCTCCAT GCAGCTCATC CGCATGCTGG AGGCCAGGAA GGCATACGTG 
AACATGGCCC TGTTTGAGAA CTTCACCTAC GCAGGGATTG ATGCCACCGC GGAGGAGGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_422400.4
CDS	464..3886
Translation	MCWIFSCARFGTHFKDMGLQFYLLLLLPWMAGAILDITLIANVQSLSHSDFFLSCIMGERDVSYLQIERENKIVMTHPKTGFQNYRNRSNYVQARGFSMPDLVGILYCLGRTPTEQAQVVYVHNSHNAHLFPVKATQSVNVGEPATFSARVLRRKEMDVMWKRNGTYYQTTDRGEVRGDLFTLTLPNVSVTENGVYSATFMGDSPLWSAFYRLIVRACAAKKWGPSCEKDCPDCLNGGICHDHVGECICPPGFTGTRCERACREGQFGRNCQETCQRAQGCRGLSFCLPDPYGCSCASGWSGSRCSQACPPGYYGPDCALMCACRNGGSCNRFSGCVCPAGWHGQHCEKSDRFPQIIKLASELEFNLGSEPIISCVATGNPLPASDGVELRKADGTMLKLVKAIIESGQITCEFQVQHLTKADAGLWECRVSTTGGQDSRKVKVNIRVPPAPLSPPRLLAKQSRQLVVSPVDCFSGDGPIVSVKLLYKPKDDASVWSSIVVDNSENITLMNLRPVTAYVVKVQLSRPGDGGEGSKGPEAVMVTECLEPTVKPVIEGWSIEEKNTLHVNWKLPSNHEPAHGFIVHLFDSAKQLVYKKNITSISVLSAHIGGLEFNKEYGLEVLVYHCTSLGPPSDLYKVMINSKGPSSPRSLSAESVSDTAIRLYWQVPEYPNGGITKYIVELQQVGGTSEPQWIDTDNGAETTKIIGGLNASTTYQFRVRANSHVPGEWSQLVKAKTLGDGALSVPPSLGSQSTEQAGVDQQLLLAIIGSVSVTCLTILFALLALFLIKKNFFHRRRTFTYQSGSGEETILQFNSGTLTLTRRPKPQPEPLSYPILEWEDIKFEDMIGEGNFGQVIRAMIKKDGLKMNAAIKMLKEFASENDHRDFAGELEVLCKLGHHPNIINLLGACENKGYLYIAIEYAPYGNLLDFLRKSRVLETDPAFAKEHGTASTLTSQQLLQFASDVAKGMQYLSEKQFIHRDLAARNILVGENLASKIADFGLSRGEEVYVKKTMGRLPVRWMAIESLNYSVYTTKSDVWSFGVLLWEIVSLGGTPYCGMTCAELYEKLPQGYRMEKPRNCDDEVYELMRQCWRDRPYERPPFAQISMQLIRMLEARKAYVNMALFENFTYAGIDATAEEA

Target ORF information:

RefSeq Version	XM_422400.6
Organism	Gallus gallus(chicken)
Definition	Gallus gallus tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_422400.6

ORF Insert Sequence:

ATGTGCTGGA TATTTTCCTG TGCCAGATTT GGAACACACT TCAAGGACAT GGGACTCCAG 
TTTTATCTAC TGCTTCTCCT CCCATGGATG GCAGGGGCCA TCCTAGACAT CACCCTGATT 
GCCAACGTGC AGAGCCTGTC CCACTCTGAC TTCTTCCTCT CCTGCATCAT GGGCGAGCGT 
GACGTGAGCT ACCTGCAGAT TGAGAGGGAG AACAAGATTG TGATGACGCA CCCCAAGACA 
GGCTTCCAAA ACTACCGCAA CCGCAGCAAC TACGTCCAGG CCAGGGGCTT CTCCATGCCT 
GACCTGGTGG GCATCCTCTA CTGCCTGGGG CGCACGCCAA CAGAGCAGGC CCAGGTGGTC 
TACGTGCACA ACAGCCACAA TGCCCACCTC TTCCCAGTGA AGGCCACGCA GTCTGTGAAT 
GTCGGCGAGC CGGCCACCTT CTCCGCCAGG GTCCTCAGGA GGAAGGAGAT GGATGTCATG 
TGGAAAAGAA ATGGTACCTA CTACCAGACC ACGGACCGGG GCGAGGTGCG GGGTGACCTT 
TTCACTCTAA CACTCCCGAA TGTCAGCGTA ACTGAAAATG GTGTCTACAG TGCCACTTTC 
ATGGGAGATA GCCCTCTGTG GAGTGCCTTC TACAGGCTAA TCGTCAGAGC GTGTGCTGCA 
AAGAAATGGG GTCCGTCCTG TGAGAAGGAC TGTCCTGACT GCCTGAATGG GGGCATCTGT 
CATGACCACG TCGGTGAATG CATCTGTCCT CCTGGCTTCA CAGGCACCCG TTGTGAGAGA 
GCCTGCCGGG AAGGCCAGTT TGGCCGCAAC TGTCAGGAGA CGTGCCAGAG AGCCCAGGGC 
TGCAGGGGGC TGAGCTTCTG CCTGCCGGAC CCCTATGGCT GCTCCTGCGC CTCGGGGTGG 
AGCGGCTCCC GCTGCAGCCA AGCCTGTCCC CCAGGATATT ATGGCCCCGA CTGCGCTCTG 
ATGTGTGCCT GCCGGAATGG TGGCAGCTGT AACCGCTTCA GTGGCTGTGT CTGCCCTGCA 
GGCTGGCATG GGCAGCACTG CGAGAAGTCA GACCGCTTCC CCCAGATCAT CAAACTGGCC 
TCGGAGCTGG AGTTCAACCT GGGCTCAGAG CCCATCATCA GCTGCGTGGC CACTGGCAAC 
CCACTGCCTG CAAGTGATGG TGTGGAGCTG CGCAAGGCTG ATGGCACCAT GCTCAAGCTG 
GTCAAAGCCA TCATAGAGTC GGGGCAGATC ACGTGCGAGT TTCAGGTGCA GCATCTGACG 
AAGGCCGACG CAGGGCTCTG GGAGTGCCGA GTCTCTACTA CTGGGGGCCA GGACAGCCGG 
AAAGTCAAGG TCAACATTCG AGTGCCACCA GCACCCCTGA GCCCCCCCCG GCTGCTGGCC 
AAGCAGAGTC GCCAGCTTGT GGTGTCACCT GTGGACTGCT TCTCTGGTGA TGGGCCCATT 
GTCTCTGTCA AGTTACTCTA CAAGCCCAAG GATGATGCCT CGGTGTGGTC GTCCATTGTG 
GTTGACAACA GTGAAAACAT CACCCTCATG AACCTCCGGC CGGTGACCGC CTATGTTGTC 
AAGGTACAGC TGAGCCGACC AGGGGATGGT GGTGAGGGCA GCAAGGGACC TGAGGCCGTC 
ATGGTGACTG AGTGCCTAGA GCCCACAGTC AAGCCTGTGA TTGAAGGTTG GTCCATTGAG 
GAGAAGAACA CCCTTCATGT CAACTGGAAA TTGCCGAGTA ACCATGAGCC AGCACATGGA 
TTCATCGTCC ATCTCTTCGA CTCCGCAAAG CAACTGGTCT ACAAGAAAAA CATCACGTCC 
ATTTCCGTAC TCTCTGCCCA CATTGGGGGC CTGGAGTTCA ACAAGGAGTA TGGGCTGGAG 
GTGCTGGTGT ACCACTGCAC CAGCCTGGGG CCCCCCTCTG ACCTCTACAA GGTCATGATC 
AACAGCAAAG GGCCTTCCTC CCCACGGTCA CTCTCAGCAG AGTCTGTGTC TGACACAGCC 
ATCAGGCTCT ACTGGCAGGT CCCCGAGTAC CCCAACGGGG GCATCACCAA GTACATTGTG 
GAGCTGCAGC AGGTGGGGGG GACCAGTGAG CCCCAGTGGA TTGACACCGA CAATGGCGCC 
GAGACCACCA AGATCATTGG AGGCCTCAAC GCCAGCACCA CCTACCAGTT TCGTGTCCGG 
GCCAACTCCC ATGTCCCAGG GGAGTGGAGC CAGCTGGTGA AAGCCAAAAC CCTGGGGGAT 
GGAGCACTGA GTGTGCCACC CAGCCTGGGC AGCCAGAGCA CCGAGCAGGC AGGAGTAGAC 
CAGCAGCTGC TCTTGGCCAT CATCGGCTCC GTGTCCGTCA CCTGCCTCAC CATCCTCTTT 
GCCCTCCTGG CACTTTTTCT CATCAAGAAG AACTTTTTCC ACCGGCGCCG CACCTTCACA 
TACCAGTCTG GCTCGGGAGA AGAGACCATC CTGCAGTTCA ATTCAGGGAC CCTGACCCTG 
ACGCGCCGGC CCAAGCCACA GCCCGAACCC CTCAGCTACC CCATCCTGGA GTGGGAGGAC 
ATCAAGTTTG AGGACATGAT TGGGGAAGGG AACTTTGGGC AGGTCATCAG GGCCATGATC 
AAAAAAGATG GCTTGAAAAT GAATGCAGCC ATCAAGATGC TGAAGGAGTT CGCCTCAGAG 
AATGACCACC GGGACTTTGC TGGAGAGTTA GAGGTGCTGT GCAAGCTGGG CCATCACCCC 
AACATAATCA ACTTGCTAGG CGCCTGTGAG AACAAGGGTT ACCTGTATAT TGCCATCGAG 
TATGCCCCCT ATGGAAACCT CCTCGACTTC CTCCGCAAAA GCCGAGTCCT GGAGACCGAC 
CCAGCCTTTG CCAAGGAGCA TGGCACCGCG TCCACCCTCA CCTCCCAGCA GCTCCTCCAG 
TTCGCTTCAG ACGTGGCCAA GGGGATGCAG TACCTAAGTG AGAAGCAGTT CATTCACAGG 
GACCTGGCAG CCAGGAATAT CCTGGTGGGA GAAAACCTGG CCTCCAAGAT CGCTGACTTT 
GGCCTTTCCA GGGGGGAGGA GGTCTACGTG AAGAAGACAA TGGGCCGCTT GCCAGTTCGT 
TGGATGGCCA TCGAGTCCCT CAACTACAGC GTGTACACCA CCAAGAGCGA TGTGTGGTCC 
TTTGGCGTCC TTCTCTGGGA GATCGTCAGC TTGGGGGGCA CGCCGTACTG CGGGATGACG 
TGTGCTGAGC TCTACGAGAA GCTGCCCCAG GGCTATCGCA TGGAGAAACC CCGCAACTGT 
GATGATGAGG TGTACGAGCT GATGAGGCAG TGCTGGCGCG ACCGTCCCTA CGAGCGCCCA 
CCCTTTGCCC AGATCTCCAT GCAGCTCATC CGCATGCTGG AGGCCAGGAA GGCATACGTG 
AACATGGCCC TGTTTGAGAA CTTCACCTAC GCAGGGATTG ATGCCACCGC GGAGGAGGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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TIE1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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