SEMA5B cDNA ORF clone, Gallus gallus(chicken)

The following SEMA5B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEMA5B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa38686 XM_015289993.2
Latest version!
Gallus gallus semaphorin 5B (SEMA5B), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OGa38685 XM_015289992.2
Latest version!
Gallus gallus semaphorin 5B (SEMA5B), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa11314 XM_015289989.2
Latest version!
Gallus gallus semaphorin 5B (SEMA5B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OGa11314 XM_015289990.2
Latest version!
Gallus gallus semaphorin 5B (SEMA5B), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OGa11314 XM_004942927.3
Latest version!
Gallus gallus semaphorin 5B (SEMA5B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OGa11314 XM_025152596.1
Latest version!
Gallus gallus semaphorin 5B (SEMA5B), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OGa11316 XM_004942929.3
Latest version!
Gallus gallus semaphorin 5B (SEMA5B), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OGa11317 XM_015289991.2
Latest version!
Gallus gallus semaphorin 5B (SEMA5B), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OGa11316 XM_004942929.2 Gallus gallus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (SEMA5B), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OGa11317 XM_015289991.1 Gallus gallus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (SEMA5B), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OGa38686 XM_015289993.1 Gallus gallus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (SEMA5B), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OGa38685 XM_015289992.1 Gallus gallus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (SEMA5B), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa11314 XM_015289990.1 Gallus gallus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (SEMA5B), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OGa11314 XM_004942927.2 Gallus gallus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (SEMA5B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OGa11314 XM_015289989.1 Gallus gallus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (SEMA5B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa38686
    Clone ID Related Accession (Same CDS sequence) XM_015289993.2 , XM_015289993.1
    Accession Version XM_015289993.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2649bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product semaphorin-5B isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289993.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGTTGTTT CAAGATTAAA GGCCATTTCC CTGTCATTGC CAAGCCTTTT TCTTCTTGCT 
    TTTCATCTCT CTGCATCACA GAATGTACCT GAGTACTCTG AAGCTGAACA TCAGCAGTGT
    GTCAGGAAAG AACACCCCAC AATCGCTTTT GAAGATCTGA AGCCTTGGGT GTCCAATTTC
    ACCTATCCAG GTGTGCATGA TTTTTCTCAG CTCGCCCTGG ATGCCAACAG GAACCAGCTC
    ATTGTGGGAG CAAGGAACTA CCTCTTCAGA CTTAGTCTAC ACAACGTTTC TCTTATTCAG
    GCAACAGCAT GGGGTTCAGA TGAAGACACC AGGAGGTCCT GTCAAAGTAA AGGGAAAACA
    GAGGAGGAGT GCCAGAACTA TGTCCGAGTG CTGATTGTTT ATGGCAAGAA GGTTTTTACC
    TGTGGTACCA ATGCCTTTTC ACCAGTGTGT TCCAGCCGTC AGGTAGGAAA TCTTAGCAAA
    ATCATTGACA GAATTAATGG AGTGGCTCGG TGCCCATATG ACCCCCGGCA TAACTCAACA
    GCTGTGATTA CATCACGAGG AGAACTCTAT GCTGCAACTG TAATTGATTT CTCTGGACGG
    GATCCTGCTA TTTACCGCAG CCTTGGAAAT GTGCCCCCAC TCAGGACAGC ACAGTACAAC
    TCCAAATGGC TCAATGAGCC TAACTTTATC GCTGCCTATG ACATCGGTTT GTTCACCTAC
    TTCTTCTTTC GAGAGAATGC GGTAGAACAC GATTGTGGGA AAACAGTATA TTCTCGTGTG
    GCCAGGGTCT GCAAAAACGA CATTGGAGGG AGATTTTTGC TGGAGGACAC GTGGACAACT
    TTCATGAAAG CCAGGCTGAA CTGCTCCAGA GCTGGAGAAA TCCCTTTCTA TTATAATGAA
    CTGCAAAGCA CCTTCTACCT TCCTGAACAA GACCTGATCT ATGGTGTCTT CACTACTAAT
    GTAAACAGCA TAGCTGCCTC AGCAGTGTGT GCCTTCAATC TCAGTGCCAT TACTCAGGCA
    TTTAATGGTC CTTTCCGTTA CCAAGAGAAC CCAAGATCAG CCTGGCTGCC AACAGCAAAC
    CCCATCCCAA ATTTCCAGTG TGGGACACTG AGCGATGACA GCCCGAATGA GAACCTGACC
    GAGAGAGTCC TACAGGACGC GCAGCGCTTG TTCCTGATGA ACGACGTGGT GCAGCCGGTC
    ACTGTAGATC CGTATGTGAC TCAGGACAGC ATTCGATTCT CTAAACTAGT GGTTGATATT
    GTTCAGGGGA AGGATACTCT CTACCACGTG ATGTACATTG GAACAGAGTA TGGCACCATC
    TTGAAGGCCC TTTCTACCAC TAATAGGAGC CTGAGGAGTT GTTATTTAGA GGAAATGCAG
    ATCCTCCCTG ATGGACAACG GGAAGCAATC AAGAGTCTCC AAATCCTGCA CAGCGACAGA
    TCGCTCTTTG TGGGCTTAAA TAACGGAGTG CTGAAAATTC CTCTGGAGAG ATGCTCCATG
    TACAGAACAG AAGGGGAATG CCTAGGAGCC AGAGACCCTT ACTGTGGCTG GGATAACAAG
    CAGAAGCGAT GCACCACCAT TGAGGACAGC TCCAACATGA GCCTATGGAC TCAAAATATT
    ACTGAGTGCC CAGTGAAAAA CCTGACAACA AATGGAAGAC TGGGGCCCTG GTCCCCATGG
    CAACCGTGTG AGCATTCAGA TGGGGACAGC ACAGGCTCCT GTATGTGTAG GTCACGTTCG
    TGTGACAGCC CTCGTCCTCG CTGTGGAGGT CGCAGCTGTG AAGGGGCCAG GATCGAGGTC
    GCAAATTGTT CAAGAAATGG CGCTTGGACA CCCTGGTCTT CCTGGGCCCT GTGCAGCACC
    TCCTGTGGGA TAGGCTTTCA GGTCCGCCAG CGGTCCTGCA GCAACCCTGC TCCACGGTAT
    GGGGGCAGGG TCTGTGTTGG ACAGAGCAGG GAAGAGAGAT TCTGTAATGA GAATAGTCCA
    TGTCCATTGC CAATTTTTTG GTCTTCGTGG GGTCCCTGGA ATAAATGTAG TGTGAATTGT
    GGTGGAGGAA TTCACTCAAG GCAAAGGACC TGTGAAAATG GAAATACATG TCCAGGCTGT
    GCTGTGGAAT ATAAGACCTG CAACCCAGAG AGCTGCCCAG AAGTGCGTAG GAACACACCC
    TGGACCCCTT GGATGCCTGT CAACATCACC CAGAATGGTG CCCGCCAGGA GCAGCGCTTC
    CGCTATACGT GTCGTGCCCA GCTGTCTGAT CCCCATGAGC TGCAGTTTGG GAGGAAGAAA
    ACTGAGAGTC GATTCTGTCC CAATGATGGC TCAGCTATGT GTGAAACTGA CTCTCTTGTT
    GACGACCTCC TCAAGACTGG TAAGACCTCT GCACGGATTA TCAATGGGGG CTGGTCCTTT
    TGGGGGGCCT GGTCTTCCTG TTCCAGGGAC TGTGAGTTGG GCTTTAGAAT CCGGAAAAGA
    ACATGCACAA ACCCTGAACC TAAAAATGGT GGCTTGCCTT GCGTGGGGTC AGCGATGGAG
    TACCAAGACT GCAACCCACA TCCTTGCCCA GTTACCATTT CCTGCACAGC CAGCAGGGAG
    TATTCTTGCC TGGTTTCCTG GTTTTCAAGT TTGTCCTCAT GTTTATGTTG CACTCCTTGT
    CCAGAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145479.1
    CDS598..3246
    Translation

    Target ORF information:

    RefSeq Version XM_015289993.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus semaphorin 5B (SEMA5B), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289993.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGTTGTTT CAAGATTAAA GGCCATTTCC CTGTCATTGC CAAGCCTTTT TCTTCTTGCT 
    TTTCATCTCT CTGCATCACA GAATGTACCT GAGTACTCTG AAGCTGAACA TCAGCAGTGT
    GTCAGGAAAG AACACCCCAC AATCGCTTTT GAAGATCTGA AGCCTTGGGT GTCCAATTTC
    ACCTATCCAG GTGTGCATGA TTTTTCTCAG CTCGCCCTGG ATGCCAACAG GAACCAGCTC
    ATTGTGGGAG CAAGGAACTA CCTCTTCAGA CTTAGTCTAC ACAACGTTTC TCTTATTCAG
    GCAACAGCAT GGGGTTCAGA TGAAGACACC AGGAGGTCCT GTCAAAGTAA AGGGAAAACA
    GAGGAGGAGT GCCAGAACTA TGTCCGAGTG CTGATTGTTT ATGGCAAGAA GGTTTTTACC
    TGTGGTACCA ATGCCTTTTC ACCAGTGTGT TCCAGCCGTC AGGTAGGAAA TCTTAGCAAA
    ATCATTGACA GAATTAATGG AGTGGCTCGG TGCCCATATG ACCCCCGGCA TAACTCAACA
    GCTGTGATTA CATCACGAGG AGAACTCTAT GCTGCAACTG TAATTGATTT CTCTGGACGG
    GATCCTGCTA TTTACCGCAG CCTTGGAAAT GTGCCCCCAC TCAGGACAGC ACAGTACAAC
    TCCAAATGGC TCAATGAGCC TAACTTTATC GCTGCCTATG ACATCGGTTT GTTCACCTAC
    TTCTTCTTTC GAGAGAATGC GGTAGAACAC GATTGTGGGA AAACAGTATA TTCTCGTGTG
    GCCAGGGTCT GCAAAAACGA CATTGGAGGG AGATTTTTGC TGGAGGACAC GTGGACAACT
    TTCATGAAAG CCAGGCTGAA CTGCTCCAGA GCTGGAGAAA TCCCTTTCTA TTATAATGAA
    CTGCAAAGCA CCTTCTACCT TCCTGAACAA GACCTGATCT ATGGTGTCTT CACTACTAAT
    GTAAACAGCA TAGCTGCCTC AGCAGTGTGT GCCTTCAATC TCAGTGCCAT TACTCAGGCA
    TTTAATGGTC CTTTCCGTTA CCAAGAGAAC CCAAGATCAG CCTGGCTGCC AACAGCAAAC
    CCCATCCCAA ATTTCCAGTG TGGGACACTG AGCGATGACA GCCCGAATGA GAACCTGACC
    GAGAGAGTCC TACAGGACGC GCAGCGCTTG TTCCTGATGA ACGACGTGGT GCAGCCGGTC
    ACTGTAGATC CGTATGTGAC TCAGGACAGC ATTCGATTCT CTAAACTAGT GGTTGATATT
    GTTCAGGGGA AGGATACTCT CTACCACGTG ATGTACATTG GAACAGAGTA TGGCACCATC
    TTGAAGGCCC TTTCTACCAC TAATAGGAGC CTGAGGAGTT GTTATTTAGA GGAAATGCAG
    ATCCTCCCTG ATGGACAACG GGAAGCAATC AAGAGTCTCC AAATCCTGCA CAGCGACAGA
    TCGCTCTTTG TGGGCTTAAA TAACGGAGTG CTGAAAATTC CTCTGGAGAG ATGCTCCATG
    TACAGAACAG AAGGGGAATG CCTAGGAGCC AGAGACCCTT ACTGTGGCTG GGATAACAAG
    CAGAAGCGAT GCACCACCAT TGAGGACAGC TCCAACATGA GCCTATGGAC TCAAAATATT
    ACTGAGTGCC CAGTGAAAAA CCTGACAACA AATGGAAGAC TGGGGCCCTG GTCCCCATGG
    CAACCGTGTG AGCATTCAGA TGGGGACAGC ACAGGCTCCT GTATGTGTAG GTCACGTTCG
    TGTGACAGCC CTCGTCCTCG CTGTGGAGGT CGCAGCTGTG AAGGGGCCAG GATCGAGGTC
    GCAAATTGTT CAAGAAATGG CGCTTGGACA CCCTGGTCTT CCTGGGCCCT GTGCAGCACC
    TCCTGTGGGA TAGGCTTTCA GGTCCGCCAG CGGTCCTGCA GCAACCCTGC TCCACGGTAT
    GGGGGCAGGG TCTGTGTTGG ACAGAGCAGG GAAGAGAGAT TCTGTAATGA GAATAGTCCA
    TGTCCATTGC CAATTTTTTG GTCTTCGTGG GGTCCCTGGA ATAAATGTAG TGTGAATTGT
    GGTGGAGGAA TTCACTCAAG GCAAAGGACC TGTGAAAATG GAAATACATG TCCAGGCTGT
    GCTGTGGAAT ATAAGACCTG CAACCCAGAG AGCTGCCCAG AAGTGCGTAG GAACACACCC
    TGGACCCCTT GGATGCCTGT CAACATCACC CAGAATGGTG CCCGCCAGGA GCAGCGCTTC
    CGCTATACGT GTCGTGCCCA GCTGTCTGAT CCCCATGAGC TGCAGTTTGG GAGGAAGAAA
    ACTGAGAGTC GATTCTGTCC CAATGATGGC TCAGCTATGT GTGAAACTGA CTCTCTTGTT
    GACGACCTCC TCAAGACTGG TAAGACCTCT GCACGGATTA TCAATGGGGG CTGGTCCTTT
    TGGGGGGCCT GGTCTTCCTG TTCCAGGGAC TGTGAGTTGG GCTTTAGAAT CCGGAAAAGA
    ACATGCACAA ACCCTGAACC TAAAAATGGT GGCTTGCCTT GCGTGGGGTC AGCGATGGAG
    TACCAAGACT GCAACCCACA TCCTTGCCCA GTTACCATTT CCTGCACAGC CAGCAGGGAG
    TATTCTTGCC TGGTTTCCTG GTTTTCAAGT TTGTCCTCAT GTTTATGTTG CACTCCTTGT
    CCAGAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa38686
    Clone ID Related Accession (Same CDS sequence) XM_015289993.2 , XM_015289993.1
    Accession Version XM_015289993.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2649bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product semaphorin-5B isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGTTGTTT CAAGATTAAA GGCCATTTCC CTGTCATTGC CAAGCCTTTT TCTTCTTGCT 
    TTTCATCTCT CTGCATCACA GAATGTACCT GAGTACTCTG AAGCTGAACA TCAGCAGTGT
    GTCAGGAAAG AACACCCCAC AATCGCTTTT GAAGATCTGA AGCCTTGGGT GTCCAATTTC
    ACCTATCCAG GTGTGCATGA TTTTTCTCAG CTCGCCCTGG ATGCCAACAG GAACCAGCTC
    ATTGTGGGAG CAAGGAACTA CCTCTTCAGA CTTAGTCTAC ACAACGTTTC TCTTATTCAG
    GCAACAGCAT GGGGTTCAGA TGAAGACACC AGGAGGTCCT GTCAAAGTAA AGGGAAAACA
    GAGGAGGAGT GCCAGAACTA TGTCCGAGTG CTGATTGTTT ATGGCAAGAA GGTTTTTACC
    TGTGGTACCA ATGCCTTTTC ACCAGTGTGT TCCAGCCGTC AGGTAGGAAA TCTTAGCAAA
    ATCATTGACA GAATTAATGG AGTGGCTCGG TGCCCATATG ACCCCCGGCA TAACTCAACA
    GCTGTGATTA CATCACGAGG AGAACTCTAT GCTGCAACTG TAATTGATTT CTCTGGACGG
    GATCCTGCTA TTTACCGCAG CCTTGGAAAT GTGCCCCCAC TCAGGACAGC ACAGTACAAC
    TCCAAATGGC TCAATGAGCC TAACTTTATC GCTGCCTATG ACATCGGTTT GTTCACCTAC
    TTCTTCTTTC GAGAGAATGC GGTAGAACAC GATTGTGGGA AAACAGTATA TTCTCGTGTG
    GCCAGGGTCT GCAAAAACGA CATTGGAGGG AGATTTTTGC TGGAGGACAC GTGGACAACT
    TTCATGAAAG CCAGGCTGAA CTGCTCCAGA GCTGGAGAAA TCCCTTTCTA TTATAATGAA
    CTGCAAAGCA CCTTCTACCT TCCTGAACAA GACCTGATCT ATGGTGTCTT CACTACTAAT
    GTAAACAGCA TAGCTGCCTC AGCAGTGTGT GCCTTCAATC TCAGTGCCAT TACTCAGGCA
    TTTAATGGTC CTTTCCGTTA CCAAGAGAAC CCAAGATCAG CCTGGCTGCC AACAGCAAAC
    CCCATCCCAA ATTTCCAGTG TGGGACACTG AGCGATGACA GCCCGAATGA GAACCTGACC
    GAGAGAGTCC TACAGGACGC GCAGCGCTTG TTCCTGATGA ACGACGTGGT GCAGCCGGTC
    ACTGTAGATC CGTATGTGAC TCAGGACAGC ATTCGATTCT CTAAACTAGT GGTTGATATT
    GTTCAGGGGA AGGATACTCT CTACCACGTG ATGTACATTG GAACAGAGTA TGGCACCATC
    TTGAAGGCCC TTTCTACCAC TAATAGGAGC CTGAGGAGTT GTTATTTAGA GGAAATGCAG
    ATCCTCCCTG ATGGACAACG GGAAGCAATC AAGAGTCTCC AAATCCTGCA CAGCGACAGA
    TCGCTCTTTG TGGGCTTAAA TAACGGAGTG CTGAAAATTC CTCTGGAGAG ATGCTCCATG
    TACAGAACAG AAGGGGAATG CCTAGGAGCC AGAGACCCTT ACTGTGGCTG GGATAACAAG
    CAGAAGCGAT GCACCACCAT TGAGGACAGC TCCAACATGA GCCTATGGAC TCAAAATATT
    ACTGAGTGCC CAGTGAAAAA CCTGACAACA AATGGAAGAC TGGGGCCCTG GTCCCCATGG
    CAACCGTGTG AGCATTCAGA TGGGGACAGC ACAGGCTCCT GTATGTGTAG GTCACGTTCG
    TGTGACAGCC CTCGTCCTCG CTGTGGAGGT CGCAGCTGTG AAGGGGCCAG GATCGAGGTC
    GCAAATTGTT CAAGAAATGG CGCTTGGACA CCCTGGTCTT CCTGGGCCCT GTGCAGCACC
    TCCTGTGGGA TAGGCTTTCA GGTCCGCCAG CGGTCCTGCA GCAACCCTGC TCCACGGTAT
    GGGGGCAGGG TCTGTGTTGG ACAGAGCAGG GAAGAGAGAT TCTGTAATGA GAATAGTCCA
    TGTCCATTGC CAATTTTTTG GTCTTCGTGG GGTCCCTGGA ATAAATGTAG TGTGAATTGT
    GGTGGAGGAA TTCACTCAAG GCAAAGGACC TGTGAAAATG GAAATACATG TCCAGGCTGT
    GCTGTGGAAT ATAAGACCTG CAACCCAGAG AGCTGCCCAG AAGTGCGTAG GAACACACCC
    TGGACCCCTT GGATGCCTGT CAACATCACC CAGAATGGTG CCCGCCAGGA GCAGCGCTTC
    CGCTATACGT GTCGTGCCCA GCTGTCTGAT CCCCATGAGC TGCAGTTTGG GAGGAAGAAA
    ACTGAGAGTC GATTCTGTCC CAATGATGGC TCAGCTATGT GTGAAACTGA CTCTCTTGTT
    GACGACCTCC TCAAGACTGG TAAGACCTCT GCACGGATTA TCAATGGGGG CTGGTCCTTT
    TGGGGGGCCT GGTCTTCCTG TTCCAGGGAC TGTGAGTTGG GCTTTAGAAT CCGGAAAAGA
    ACATGCACAA ACCCTGAACC TAAAAATGGT GGCTTGCCTT GCGTGGGGTC AGCGATGGAG
    TACCAAGACT GCAACCCACA TCCTTGCCCA GTTACCATTT CCTGCACAGC CAGCAGGGAG
    TATTCTTGCC TGGTTTCCTG GTTTTCAAGT TTGTCCTCAT GTTTATGTTG CACTCCTTGT
    CCAGAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145479.1
    CDS608..3256
    Translation

    Target ORF information:

    RefSeq Version XM_015289993.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (SEMA5B), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289993.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGTTGTTT CAAGATTAAA GGCCATTTCC CTGTCATTGC CAAGCCTTTT TCTTCTTGCT 
    TTTCATCTCT CTGCATCACA GAATGTACCT GAGTACTCTG AAGCTGAACA TCAGCAGTGT
    GTCAGGAAAG AACACCCCAC AATCGCTTTT GAAGATCTGA AGCCTTGGGT GTCCAATTTC
    ACCTATCCAG GTGTGCATGA TTTTTCTCAG CTCGCCCTGG ATGCCAACAG GAACCAGCTC
    ATTGTGGGAG CAAGGAACTA CCTCTTCAGA CTTAGTCTAC ACAACGTTTC TCTTATTCAG
    GCAACAGCAT GGGGTTCAGA TGAAGACACC AGGAGGTCCT GTCAAAGTAA AGGGAAAACA
    GAGGAGGAGT GCCAGAACTA TGTCCGAGTG CTGATTGTTT ATGGCAAGAA GGTTTTTACC
    TGTGGTACCA ATGCCTTTTC ACCAGTGTGT TCCAGCCGTC AGGTAGGAAA TCTTAGCAAA
    ATCATTGACA GAATTAATGG AGTGGCTCGG TGCCCATATG ACCCCCGGCA TAACTCAACA
    GCTGTGATTA CATCACGAGG AGAACTCTAT GCTGCAACTG TAATTGATTT CTCTGGACGG
    GATCCTGCTA TTTACCGCAG CCTTGGAAAT GTGCCCCCAC TCAGGACAGC ACAGTACAAC
    TCCAAATGGC TCAATGAGCC TAACTTTATC GCTGCCTATG ACATCGGTTT GTTCACCTAC
    TTCTTCTTTC GAGAGAATGC GGTAGAACAC GATTGTGGGA AAACAGTATA TTCTCGTGTG
    GCCAGGGTCT GCAAAAACGA CATTGGAGGG AGATTTTTGC TGGAGGACAC GTGGACAACT
    TTCATGAAAG CCAGGCTGAA CTGCTCCAGA GCTGGAGAAA TCCCTTTCTA TTATAATGAA
    CTGCAAAGCA CCTTCTACCT TCCTGAACAA GACCTGATCT ATGGTGTCTT CACTACTAAT
    GTAAACAGCA TAGCTGCCTC AGCAGTGTGT GCCTTCAATC TCAGTGCCAT TACTCAGGCA
    TTTAATGGTC CTTTCCGTTA CCAAGAGAAC CCAAGATCAG CCTGGCTGCC AACAGCAAAC
    CCCATCCCAA ATTTCCAGTG TGGGACACTG AGCGATGACA GCCCGAATGA GAACCTGACC
    GAGAGAGTCC TACAGGACGC GCAGCGCTTG TTCCTGATGA ACGACGTGGT GCAGCCGGTC
    ACTGTAGATC CGTATGTGAC TCAGGACAGC ATTCGATTCT CTAAACTAGT GGTTGATATT
    GTTCAGGGGA AGGATACTCT CTACCACGTG ATGTACATTG GAACAGAGTA TGGCACCATC
    TTGAAGGCCC TTTCTACCAC TAATAGGAGC CTGAGGAGTT GTTATTTAGA GGAAATGCAG
    ATCCTCCCTG ATGGACAACG GGAAGCAATC AAGAGTCTCC AAATCCTGCA CAGCGACAGA
    TCGCTCTTTG TGGGCTTAAA TAACGGAGTG CTGAAAATTC CTCTGGAGAG ATGCTCCATG
    TACAGAACAG AAGGGGAATG CCTAGGAGCC AGAGACCCTT ACTGTGGCTG GGATAACAAG
    CAGAAGCGAT GCACCACCAT TGAGGACAGC TCCAACATGA GCCTATGGAC TCAAAATATT
    ACTGAGTGCC CAGTGAAAAA CCTGACAACA AATGGAAGAC TGGGGCCCTG GTCCCCATGG
    CAACCGTGTG AGCATTCAGA TGGGGACAGC ACAGGCTCCT GTATGTGTAG GTCACGTTCG
    TGTGACAGCC CTCGTCCTCG CTGTGGAGGT CGCAGCTGTG AAGGGGCCAG GATCGAGGTC
    GCAAATTGTT CAAGAAATGG CGCTTGGACA CCCTGGTCTT CCTGGGCCCT GTGCAGCACC
    TCCTGTGGGA TAGGCTTTCA GGTCCGCCAG CGGTCCTGCA GCAACCCTGC TCCACGGTAT
    GGGGGCAGGG TCTGTGTTGG ACAGAGCAGG GAAGAGAGAT TCTGTAATGA GAATAGTCCA
    TGTCCATTGC CAATTTTTTG GTCTTCGTGG GGTCCCTGGA ATAAATGTAG TGTGAATTGT
    GGTGGAGGAA TTCACTCAAG GCAAAGGACC TGTGAAAATG GAAATACATG TCCAGGCTGT
    GCTGTGGAAT ATAAGACCTG CAACCCAGAG AGCTGCCCAG AAGTGCGTAG GAACACACCC
    TGGACCCCTT GGATGCCTGT CAACATCACC CAGAATGGTG CCCGCCAGGA GCAGCGCTTC
    CGCTATACGT GTCGTGCCCA GCTGTCTGAT CCCCATGAGC TGCAGTTTGG GAGGAAGAAA
    ACTGAGAGTC GATTCTGTCC CAATGATGGC TCAGCTATGT GTGAAACTGA CTCTCTTGTT
    GACGACCTCC TCAAGACTGG TAAGACCTCT GCACGGATTA TCAATGGGGG CTGGTCCTTT
    TGGGGGGCCT GGTCTTCCTG TTCCAGGGAC TGTGAGTTGG GCTTTAGAAT CCGGAAAAGA
    ACATGCACAA ACCCTGAACC TAAAAATGGT GGCTTGCCTT GCGTGGGGTC AGCGATGGAG
    TACCAAGACT GCAACCCACA TCCTTGCCCA GTTACCATTT CCTGCACAGC CAGCAGGGAG
    TATTCTTGCC TGGTTTCCTG GTTTTCAAGT TTGTCCTCAT GTTTATGTTG CACTCCTTGT
    CCAGAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa38685
    Clone ID Related Accession (Same CDS sequence) XM_015289992.1 , XM_015289992.2
    Accession Version XM_015289992.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3201bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product semaphorin-5B isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGTTGTTT CAAGATTAAA GGCCATTTCC CTGTCATTGC CAAGCCTTTT TCTTCTTGCT 
    TTTCATCTCT CTGCATCACA GAATGTACCT GAGTACTCTG AAGCTGAACA TCAGCAGTGT
    GTCAGGAAAG AACACCCCAC AATCGCTTTT GAAGATCTGA AGCCTTGGGT GTCCAATTTC
    ACCTATCCAG GTGTGCATGA TTTTTCTCAG CTCGCCCTGG ATGCCAACAG GAACCAGCTC
    ATTGTGGGAG CAAGGAACTA CCTCTTCAGA CTTAGTCTAC ACAACGTTTC TCTTATTCAG
    GCAACAGCAT GGGGTTCAGA TGAAGACACC AGGAGGTCCT GTCAAAGTAA AGGGAAAACA
    GAGGAGGAGT GCCAGAACTA TGTCCGAGTG CTGATTGTTT ATGGCAAGAA GGTTTTTACC
    TGTGGTACCA ATGCCTTTTC ACCAGTGTGT TCCAGCCGTC AGGTAGGAAA TCTTAGCAAA
    ATCATTGACA GAATTAATGG AGTGGCTCGG TGCCCATATG ACCCCCGGCA TAACTCAACA
    GCTGTGATTA CATCACGAGG AGAACTCTAT GCTGCAACTG TAATTGATTT CTCTGGACGG
    GATCCTGCTA TTTACCGCAG CCTTGGAAAT GTGCCCCCAC TCAGGACAGC ACAGTACAAC
    TCCAAATGGC TCAATGAGCC TAACTTTATC GCTGCCTATG ACATCGGTTT GTTCACCTAC
    TTCTTCTTTC GAGAGAATGC GGTAGAACAC GATTGTGGGA AAACAGTATA TTCTCGTGTG
    GCCAGGGTCT GCAAAAACGA CATTGGAGGG AGATTTTTGC TGGAGGACAC GTGGACAACT
    TTCATGAAAG CCAGGCTGAA CTGCTCCAGA GCTGGAGAAA TCCCTTTCTA TTATAATGAA
    CTGCAAAGCA CCTTCTACCT TCCTGAACAA GACCTGATCT ATGGTGTCTT CACTACTAAT
    GTAAACAGCA TAGCTGCCTC AGCAGTGTGT GCCTTCAATC TCAGTGCCAT TACTCAGGCA
    TTTAATGGTC CTTTCCGTTA CCAAGAGAAC CCAAGATCAG CCTGGCTGCC AACAGCAAAC
    CCCATCCCAA ATTTCCAGTG TGGGACACTG AGCGATGACA GCCCGAATGA GAACCTGACC
    GAGAGAGTCC TACAGGACGC GCAGCGCTTG TTCCTGATGA ACGACGTGGT GCAGCCGGTC
    ACTGTAGATC CGTATGTGAC TCAGGACAGC ATTCGATTCT CTAAACTAGT GGTTGATATT
    GTTCAGGGGA AGGATACTCT CTACCACGTG ATGTACATTG GAACAGAGTA TGGCACCATC
    TTGAAGGCCC TTTCTACCAC TAATAGGAGC CTGAGGAGTT GTTATTTAGA GGAAATGCAG
    ATCCTCCCTG ATGGACAACG GGAAGCAATC AAGAGTCTCC AAATCCTGCA CAGCGACAGA
    TCGCTCTTTG TGGGCTTAAA TAACGGAGTG CTGAAAATTC CTCTGGAGAG ATGCTCCATG
    TACAGAACAG AAGGGGAATG CCTAGGAGCC AGAGACCCTT ACTGTGGCTG GGATAACAAG
    CAGAAGCGAT GCACCACCAT TGAGGACAGC TCCAACATGA GCCTATGGAC TCAAAATATT
    ACTGAGTGCC CAGTGAAAAA CCTGACAACA AATGGAAGAC TGGGGCCCTG GTCCCCATGG
    CAACCGTGTG AGCATTCAGA TGGGGACAGC ACAGGCTCCT GTATGTGTAG GTCACGTTCG
    TGTGACAGCC CTCGTCCTCG CTGTGGAGGT CGCAGCTGTG AAGGGGCCAG GATCGAGGTC
    GCAAATTGTT CAAGAAATGG CGCTTGGACA CCCTGGTCTT CCTGGGCCCT GTGCAGCACC
    TCCTGTGGGA TAGGCTTTCA GGTCCGCCAG CGGTCCTGCA GCAACCCTGC TCCACGGTAT
    GGGGGCAGGG TCTGTGTTGG ACAGAGCAGG GAAGAGAGAT TCTGTAATGA GAATAGTCCA
    TGTCCATTGC CAATTTTTTG GTCTTCGTGG GGTCCCTGGA ATAAATGTAG TGTGAATTGT
    GGTGGAGGAA TTCACTCAAG GCAAAGGACC TGTGAAAATG GAAATACATG TCCAGGCTGT
    GCTGTGGAAT ATAAGACCTG CAACCCAGAG AGCTGCCCAG AAGTGCGTAG GAACACACCC
    TGGACCCCTT GGATGCCTGT CAACATCACC CAGAATGGTG CCCGCCAGGA GCAGCGCTTC
    CGCTATACGT GTCGTGCCCA GCTGTCTGAT CCCCATGAGC TGCAGTTTGG GAGGAAGAAA
    ACTGAGAGTC GATTCTGTCC CAATGATGGC TCAGCTATGT GTGAAACTGA CTCTCTTGTT
    GACGACCTCC TCAAGACTGG TAAGACCTCT GCACGGATTA TCAATGGGGG CTGGTCCTTT
    TGGGGGGCCT GGTCTTCCTG TTCCAGGGAC TGTGAGTTGG GCTTTAGAAT CCGGAAAAGA
    ACATGCACAA ACCCTGAACC TAAAAATGGT GGCTTGCCTT GCGTGGGGTC AGCGATGGAG
    TACCAAGACT GCAACCCACA TCCTTGCCCA GTAAAAGGCT CATGGTCTTG CTGGACTCCT
    TGGTCTCAGT GCTCAGCAAC CTGTGGAGGA GGGCATTACC AGCGCACACG GACTTGCACC
    AACCCAGCTC CATCCAGTGG AGAGGATATC TGCATCGGGC TGCACACTGA GGAGGCTCTC
    TGCAACACCC ACCCATGTGA AGGTGGCTGG TCAGAGTGGT CAGAGTGGAG CCTGTGCAAT
    GAAGAGGGCA TCCAGTATCG CAGCCGTTAC TGTGAGGTAC ACAGCCCAGA CTCTTCTCAG
    TGCGTTGGAA ACAGCACACA ATACCAAGAT TGCCTGTATA ATGAAATTCC TGTGATCCTG
    CCAGCATCCA GCATTGATGA GAGCACAAAC TGTGGAGGTT TTAGTCTCAT CCATCTGATT
    GCCACTGGGG TCTCCTGCTT CTTTGGCTCT AGCCTCTTAA CGTTTGTGAT TTACGTGTAC
    TGCCAGCGCT GCCAGAGGCA GTCCCAGGAG TCGACCGTTA TCCACCCAAC CACTCCCAAC
    CATCTTCATT ACAAGGGCAA CACAACCCCC AAGAATGAGA AGTACACCCC CATGGAGTTC
    AAGCTGACAG AAGTGACACA CTTAATATCG GGGGGTCCAA ACCTGGATAT CTACCAATGG
    AGATGGAGAT CAGACACTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145478.1
    CDS606..3806
    Translation

    Target ORF information:

    RefSeq Version XM_015289992.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (SEMA5B), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289992.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGTTGTTT CAAGATTAAA GGCCATTTCC CTGTCATTGC CAAGCCTTTT TCTTCTTGCT 
    TTTCATCTCT CTGCATCACA GAATGTACCT GAGTACTCTG AAGCTGAACA TCAGCAGTGT
    GTCAGGAAAG AACACCCCAC AATCGCTTTT GAAGATCTGA AGCCTTGGGT GTCCAATTTC
    ACCTATCCAG GTGTGCATGA TTTTTCTCAG CTCGCCCTGG ATGCCAACAG GAACCAGCTC
    ATTGTGGGAG CAAGGAACTA CCTCTTCAGA CTTAGTCTAC ACAACGTTTC TCTTATTCAG
    GCAACAGCAT GGGGTTCAGA TGAAGACACC AGGAGGTCCT GTCAAAGTAA AGGGAAAACA
    GAGGAGGAGT GCCAGAACTA TGTCCGAGTG CTGATTGTTT ATGGCAAGAA GGTTTTTACC
    TGTGGTACCA ATGCCTTTTC ACCAGTGTGT TCCAGCCGTC AGGTAGGAAA TCTTAGCAAA
    ATCATTGACA GAATTAATGG AGTGGCTCGG TGCCCATATG ACCCCCGGCA TAACTCAACA
    GCTGTGATTA CATCACGAGG AGAACTCTAT GCTGCAACTG TAATTGATTT CTCTGGACGG
    GATCCTGCTA TTTACCGCAG CCTTGGAAAT GTGCCCCCAC TCAGGACAGC ACAGTACAAC
    TCCAAATGGC TCAATGAGCC TAACTTTATC GCTGCCTATG ACATCGGTTT GTTCACCTAC
    TTCTTCTTTC GAGAGAATGC GGTAGAACAC GATTGTGGGA AAACAGTATA TTCTCGTGTG
    GCCAGGGTCT GCAAAAACGA CATTGGAGGG AGATTTTTGC TGGAGGACAC GTGGACAACT
    TTCATGAAAG CCAGGCTGAA CTGCTCCAGA GCTGGAGAAA TCCCTTTCTA TTATAATGAA
    CTGCAAAGCA CCTTCTACCT TCCTGAACAA GACCTGATCT ATGGTGTCTT CACTACTAAT
    GTAAACAGCA TAGCTGCCTC AGCAGTGTGT GCCTTCAATC TCAGTGCCAT TACTCAGGCA
    TTTAATGGTC CTTTCCGTTA CCAAGAGAAC CCAAGATCAG CCTGGCTGCC AACAGCAAAC
    CCCATCCCAA ATTTCCAGTG TGGGACACTG AGCGATGACA GCCCGAATGA GAACCTGACC
    GAGAGAGTCC TACAGGACGC GCAGCGCTTG TTCCTGATGA ACGACGTGGT GCAGCCGGTC
    ACTGTAGATC CGTATGTGAC TCAGGACAGC ATTCGATTCT CTAAACTAGT GGTTGATATT
    GTTCAGGGGA AGGATACTCT CTACCACGTG ATGTACATTG GAACAGAGTA TGGCACCATC
    TTGAAGGCCC TTTCTACCAC TAATAGGAGC CTGAGGAGTT GTTATTTAGA GGAAATGCAG
    ATCCTCCCTG ATGGACAACG GGAAGCAATC AAGAGTCTCC AAATCCTGCA CAGCGACAGA
    TCGCTCTTTG TGGGCTTAAA TAACGGAGTG CTGAAAATTC CTCTGGAGAG ATGCTCCATG
    TACAGAACAG AAGGGGAATG CCTAGGAGCC AGAGACCCTT ACTGTGGCTG GGATAACAAG
    CAGAAGCGAT GCACCACCAT TGAGGACAGC TCCAACATGA GCCTATGGAC TCAAAATATT
    ACTGAGTGCC CAGTGAAAAA CCTGACAACA AATGGAAGAC TGGGGCCCTG GTCCCCATGG
    CAACCGTGTG AGCATTCAGA TGGGGACAGC ACAGGCTCCT GTATGTGTAG GTCACGTTCG
    TGTGACAGCC CTCGTCCTCG CTGTGGAGGT CGCAGCTGTG AAGGGGCCAG GATCGAGGTC
    GCAAATTGTT CAAGAAATGG CGCTTGGACA CCCTGGTCTT CCTGGGCCCT GTGCAGCACC
    TCCTGTGGGA TAGGCTTTCA GGTCCGCCAG CGGTCCTGCA GCAACCCTGC TCCACGGTAT
    GGGGGCAGGG TCTGTGTTGG ACAGAGCAGG GAAGAGAGAT TCTGTAATGA GAATAGTCCA
    TGTCCATTGC CAATTTTTTG GTCTTCGTGG GGTCCCTGGA ATAAATGTAG TGTGAATTGT
    GGTGGAGGAA TTCACTCAAG GCAAAGGACC TGTGAAAATG GAAATACATG TCCAGGCTGT
    GCTGTGGAAT ATAAGACCTG CAACCCAGAG AGCTGCCCAG AAGTGCGTAG GAACACACCC
    TGGACCCCTT GGATGCCTGT CAACATCACC CAGAATGGTG CCCGCCAGGA GCAGCGCTTC
    CGCTATACGT GTCGTGCCCA GCTGTCTGAT CCCCATGAGC TGCAGTTTGG GAGGAAGAAA
    ACTGAGAGTC GATTCTGTCC CAATGATGGC TCAGCTATGT GTGAAACTGA CTCTCTTGTT
    GACGACCTCC TCAAGACTGG TAAGACCTCT GCACGGATTA TCAATGGGGG CTGGTCCTTT
    TGGGGGGCCT GGTCTTCCTG TTCCAGGGAC TGTGAGTTGG GCTTTAGAAT CCGGAAAAGA
    ACATGCACAA ACCCTGAACC TAAAAATGGT GGCTTGCCTT GCGTGGGGTC AGCGATGGAG
    TACCAAGACT GCAACCCACA TCCTTGCCCA GTAAAAGGCT CATGGTCTTG CTGGACTCCT
    TGGTCTCAGT GCTCAGCAAC CTGTGGAGGA GGGCATTACC AGCGCACACG GACTTGCACC
    AACCCAGCTC CATCCAGTGG AGAGGATATC TGCATCGGGC TGCACACTGA GGAGGCTCTC
    TGCAACACCC ACCCATGTGA AGGTGGCTGG TCAGAGTGGT CAGAGTGGAG CCTGTGCAAT
    GAAGAGGGCA TCCAGTATCG CAGCCGTTAC TGTGAGGTAC ACAGCCCAGA CTCTTCTCAG
    TGCGTTGGAA ACAGCACACA ATACCAAGAT TGCCTGTATA ATGAAATTCC TGTGATCCTG
    CCAGCATCCA GCATTGATGA GAGCACAAAC TGTGGAGGTT TTAGTCTCAT CCATCTGATT
    GCCACTGGGG TCTCCTGCTT CTTTGGCTCT AGCCTCTTAA CGTTTGTGAT TTACGTGTAC
    TGCCAGCGCT GCCAGAGGCA GTCCCAGGAG TCGACCGTTA TCCACCCAAC CACTCCCAAC
    CATCTTCATT ACAAGGGCAA CACAACCCCC AAGAATGAGA AGTACACCCC CATGGAGTTC
    AAGCTGACAG AAGTGACACA CTTAATATCG GGGGGTCCAA ACCTGGATAT CTACCAATGG
    AGATGGAGAT CAGACACTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa38685
    Clone ID Related Accession (Same CDS sequence) XM_015289992.1 , XM_015289992.2
    Accession Version XM_015289992.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3201bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product semaphorin-5B isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289992.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGTTGTTT CAAGATTAAA GGCCATTTCC CTGTCATTGC CAAGCCTTTT TCTTCTTGCT 
    TTTCATCTCT CTGCATCACA GAATGTACCT GAGTACTCTG AAGCTGAACA TCAGCAGTGT
    GTCAGGAAAG AACACCCCAC AATCGCTTTT GAAGATCTGA AGCCTTGGGT GTCCAATTTC
    ACCTATCCAG GTGTGCATGA TTTTTCTCAG CTCGCCCTGG ATGCCAACAG GAACCAGCTC
    ATTGTGGGAG CAAGGAACTA CCTCTTCAGA CTTAGTCTAC ACAACGTTTC TCTTATTCAG
    GCAACAGCAT GGGGTTCAGA TGAAGACACC AGGAGGTCCT GTCAAAGTAA AGGGAAAACA
    GAGGAGGAGT GCCAGAACTA TGTCCGAGTG CTGATTGTTT ATGGCAAGAA GGTTTTTACC
    TGTGGTACCA ATGCCTTTTC ACCAGTGTGT TCCAGCCGTC AGGTAGGAAA TCTTAGCAAA
    ATCATTGACA GAATTAATGG AGTGGCTCGG TGCCCATATG ACCCCCGGCA TAACTCAACA
    GCTGTGATTA CATCACGAGG AGAACTCTAT GCTGCAACTG TAATTGATTT CTCTGGACGG
    GATCCTGCTA TTTACCGCAG CCTTGGAAAT GTGCCCCCAC TCAGGACAGC ACAGTACAAC
    TCCAAATGGC TCAATGAGCC TAACTTTATC GCTGCCTATG ACATCGGTTT GTTCACCTAC
    TTCTTCTTTC GAGAGAATGC GGTAGAACAC GATTGTGGGA AAACAGTATA TTCTCGTGTG
    GCCAGGGTCT GCAAAAACGA CATTGGAGGG AGATTTTTGC TGGAGGACAC GTGGACAACT
    TTCATGAAAG CCAGGCTGAA CTGCTCCAGA GCTGGAGAAA TCCCTTTCTA TTATAATGAA
    CTGCAAAGCA CCTTCTACCT TCCTGAACAA GACCTGATCT ATGGTGTCTT CACTACTAAT
    GTAAACAGCA TAGCTGCCTC AGCAGTGTGT GCCTTCAATC TCAGTGCCAT TACTCAGGCA
    TTTAATGGTC CTTTCCGTTA CCAAGAGAAC CCAAGATCAG CCTGGCTGCC AACAGCAAAC
    CCCATCCCAA ATTTCCAGTG TGGGACACTG AGCGATGACA GCCCGAATGA GAACCTGACC
    GAGAGAGTCC TACAGGACGC GCAGCGCTTG TTCCTGATGA ACGACGTGGT GCAGCCGGTC
    ACTGTAGATC CGTATGTGAC TCAGGACAGC ATTCGATTCT CTAAACTAGT GGTTGATATT
    GTTCAGGGGA AGGATACTCT CTACCACGTG ATGTACATTG GAACAGAGTA TGGCACCATC
    TTGAAGGCCC TTTCTACCAC TAATAGGAGC CTGAGGAGTT GTTATTTAGA GGAAATGCAG
    ATCCTCCCTG ATGGACAACG GGAAGCAATC AAGAGTCTCC AAATCCTGCA CAGCGACAGA
    TCGCTCTTTG TGGGCTTAAA TAACGGAGTG CTGAAAATTC CTCTGGAGAG ATGCTCCATG
    TACAGAACAG AAGGGGAATG CCTAGGAGCC AGAGACCCTT ACTGTGGCTG GGATAACAAG
    CAGAAGCGAT GCACCACCAT TGAGGACAGC TCCAACATGA GCCTATGGAC TCAAAATATT
    ACTGAGTGCC CAGTGAAAAA CCTGACAACA AATGGAAGAC TGGGGCCCTG GTCCCCATGG
    CAACCGTGTG AGCATTCAGA TGGGGACAGC ACAGGCTCCT GTATGTGTAG GTCACGTTCG
    TGTGACAGCC CTCGTCCTCG CTGTGGAGGT CGCAGCTGTG AAGGGGCCAG GATCGAGGTC
    GCAAATTGTT CAAGAAATGG CGCTTGGACA CCCTGGTCTT CCTGGGCCCT GTGCAGCACC
    TCCTGTGGGA TAGGCTTTCA GGTCCGCCAG CGGTCCTGCA GCAACCCTGC TCCACGGTAT
    GGGGGCAGGG TCTGTGTTGG ACAGAGCAGG GAAGAGAGAT TCTGTAATGA GAATAGTCCA
    TGTCCATTGC CAATTTTTTG GTCTTCGTGG GGTCCCTGGA ATAAATGTAG TGTGAATTGT
    GGTGGAGGAA TTCACTCAAG GCAAAGGACC TGTGAAAATG GAAATACATG TCCAGGCTGT
    GCTGTGGAAT ATAAGACCTG CAACCCAGAG AGCTGCCCAG AAGTGCGTAG GAACACACCC
    TGGACCCCTT GGATGCCTGT CAACATCACC CAGAATGGTG CCCGCCAGGA GCAGCGCTTC
    CGCTATACGT GTCGTGCCCA GCTGTCTGAT CCCCATGAGC TGCAGTTTGG GAGGAAGAAA
    ACTGAGAGTC GATTCTGTCC CAATGATGGC TCAGCTATGT GTGAAACTGA CTCTCTTGTT
    GACGACCTCC TCAAGACTGG TAAGACCTCT GCACGGATTA TCAATGGGGG CTGGTCCTTT
    TGGGGGGCCT GGTCTTCCTG TTCCAGGGAC TGTGAGTTGG GCTTTAGAAT CCGGAAAAGA
    ACATGCACAA ACCCTGAACC TAAAAATGGT GGCTTGCCTT GCGTGGGGTC AGCGATGGAG
    TACCAAGACT GCAACCCACA TCCTTGCCCA GTAAAAGGCT CATGGTCTTG CTGGACTCCT
    TGGTCTCAGT GCTCAGCAAC CTGTGGAGGA GGGCATTACC AGCGCACACG GACTTGCACC
    AACCCAGCTC CATCCAGTGG AGAGGATATC TGCATCGGGC TGCACACTGA GGAGGCTCTC
    TGCAACACCC ACCCATGTGA AGGTGGCTGG TCAGAGTGGT CAGAGTGGAG CCTGTGCAAT
    GAAGAGGGCA TCCAGTATCG CAGCCGTTAC TGTGAGGTAC ACAGCCCAGA CTCTTCTCAG
    TGCGTTGGAA ACAGCACACA ATACCAAGAT TGCCTGTATA ATGAAATTCC TGTGATCCTG
    CCAGCATCCA GCATTGATGA GAGCACAAAC TGTGGAGGTT TTAGTCTCAT CCATCTGATT
    GCCACTGGGG TCTCCTGCTT CTTTGGCTCT AGCCTCTTAA CGTTTGTGAT TTACGTGTAC
    TGCCAGCGCT GCCAGAGGCA GTCCCAGGAG TCGACCGTTA TCCACCCAAC CACTCCCAAC
    CATCTTCATT ACAAGGGCAA CACAACCCCC AAGAATGAGA AGTACACCCC CATGGAGTTC
    AAGCTGACAG AAGTGACACA CTTAATATCG GGGGGTCCAA ACCTGGATAT CTACCAATGG
    AGATGGAGAT CAGACACTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145478.1
    CDS597..3797
    Translation

    Target ORF information:

    RefSeq Version XM_015289992.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus semaphorin 5B (SEMA5B), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289992.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGTTGTTT CAAGATTAAA GGCCATTTCC CTGTCATTGC CAAGCCTTTT TCTTCTTGCT 
    TTTCATCTCT CTGCATCACA GAATGTACCT GAGTACTCTG AAGCTGAACA TCAGCAGTGT
    GTCAGGAAAG AACACCCCAC AATCGCTTTT GAAGATCTGA AGCCTTGGGT GTCCAATTTC
    ACCTATCCAG GTGTGCATGA TTTTTCTCAG CTCGCCCTGG ATGCCAACAG GAACCAGCTC
    ATTGTGGGAG CAAGGAACTA CCTCTTCAGA CTTAGTCTAC ACAACGTTTC TCTTATTCAG
    GCAACAGCAT GGGGTTCAGA TGAAGACACC AGGAGGTCCT GTCAAAGTAA AGGGAAAACA
    GAGGAGGAGT GCCAGAACTA TGTCCGAGTG CTGATTGTTT ATGGCAAGAA GGTTTTTACC
    TGTGGTACCA ATGCCTTTTC ACCAGTGTGT TCCAGCCGTC AGGTAGGAAA TCTTAGCAAA
    ATCATTGACA GAATTAATGG AGTGGCTCGG TGCCCATATG ACCCCCGGCA TAACTCAACA
    GCTGTGATTA CATCACGAGG AGAACTCTAT GCTGCAACTG TAATTGATTT CTCTGGACGG
    GATCCTGCTA TTTACCGCAG CCTTGGAAAT GTGCCCCCAC TCAGGACAGC ACAGTACAAC
    TCCAAATGGC TCAATGAGCC TAACTTTATC GCTGCCTATG ACATCGGTTT GTTCACCTAC
    TTCTTCTTTC GAGAGAATGC GGTAGAACAC GATTGTGGGA AAACAGTATA TTCTCGTGTG
    GCCAGGGTCT GCAAAAACGA CATTGGAGGG AGATTTTTGC TGGAGGACAC GTGGACAACT
    TTCATGAAAG CCAGGCTGAA CTGCTCCAGA GCTGGAGAAA TCCCTTTCTA TTATAATGAA
    CTGCAAAGCA CCTTCTACCT TCCTGAACAA GACCTGATCT ATGGTGTCTT CACTACTAAT
    GTAAACAGCA TAGCTGCCTC AGCAGTGTGT GCCTTCAATC TCAGTGCCAT TACTCAGGCA
    TTTAATGGTC CTTTCCGTTA CCAAGAGAAC CCAAGATCAG CCTGGCTGCC AACAGCAAAC
    CCCATCCCAA ATTTCCAGTG TGGGACACTG AGCGATGACA GCCCGAATGA GAACCTGACC
    GAGAGAGTCC TACAGGACGC GCAGCGCTTG TTCCTGATGA ACGACGTGGT GCAGCCGGTC
    ACTGTAGATC CGTATGTGAC TCAGGACAGC ATTCGATTCT CTAAACTAGT GGTTGATATT
    GTTCAGGGGA AGGATACTCT CTACCACGTG ATGTACATTG GAACAGAGTA TGGCACCATC
    TTGAAGGCCC TTTCTACCAC TAATAGGAGC CTGAGGAGTT GTTATTTAGA GGAAATGCAG
    ATCCTCCCTG ATGGACAACG GGAAGCAATC AAGAGTCTCC AAATCCTGCA CAGCGACAGA
    TCGCTCTTTG TGGGCTTAAA TAACGGAGTG CTGAAAATTC CTCTGGAGAG ATGCTCCATG
    TACAGAACAG AAGGGGAATG CCTAGGAGCC AGAGACCCTT ACTGTGGCTG GGATAACAAG
    CAGAAGCGAT GCACCACCAT TGAGGACAGC TCCAACATGA GCCTATGGAC TCAAAATATT
    ACTGAGTGCC CAGTGAAAAA CCTGACAACA AATGGAAGAC TGGGGCCCTG GTCCCCATGG
    CAACCGTGTG AGCATTCAGA TGGGGACAGC ACAGGCTCCT GTATGTGTAG GTCACGTTCG
    TGTGACAGCC CTCGTCCTCG CTGTGGAGGT CGCAGCTGTG AAGGGGCCAG GATCGAGGTC
    GCAAATTGTT CAAGAAATGG CGCTTGGACA CCCTGGTCTT CCTGGGCCCT GTGCAGCACC
    TCCTGTGGGA TAGGCTTTCA GGTCCGCCAG CGGTCCTGCA GCAACCCTGC TCCACGGTAT
    GGGGGCAGGG TCTGTGTTGG ACAGAGCAGG GAAGAGAGAT TCTGTAATGA GAATAGTCCA
    TGTCCATTGC CAATTTTTTG GTCTTCGTGG GGTCCCTGGA ATAAATGTAG TGTGAATTGT
    GGTGGAGGAA TTCACTCAAG GCAAAGGACC TGTGAAAATG GAAATACATG TCCAGGCTGT
    GCTGTGGAAT ATAAGACCTG CAACCCAGAG AGCTGCCCAG AAGTGCGTAG GAACACACCC
    TGGACCCCTT GGATGCCTGT CAACATCACC CAGAATGGTG CCCGCCAGGA GCAGCGCTTC
    CGCTATACGT GTCGTGCCCA GCTGTCTGAT CCCCATGAGC TGCAGTTTGG GAGGAAGAAA
    ACTGAGAGTC GATTCTGTCC CAATGATGGC TCAGCTATGT GTGAAACTGA CTCTCTTGTT
    GACGACCTCC TCAAGACTGG TAAGACCTCT GCACGGATTA TCAATGGGGG CTGGTCCTTT
    TGGGGGGCCT GGTCTTCCTG TTCCAGGGAC TGTGAGTTGG GCTTTAGAAT CCGGAAAAGA
    ACATGCACAA ACCCTGAACC TAAAAATGGT GGCTTGCCTT GCGTGGGGTC AGCGATGGAG
    TACCAAGACT GCAACCCACA TCCTTGCCCA GTAAAAGGCT CATGGTCTTG CTGGACTCCT
    TGGTCTCAGT GCTCAGCAAC CTGTGGAGGA GGGCATTACC AGCGCACACG GACTTGCACC
    AACCCAGCTC CATCCAGTGG AGAGGATATC TGCATCGGGC TGCACACTGA GGAGGCTCTC
    TGCAACACCC ACCCATGTGA AGGTGGCTGG TCAGAGTGGT CAGAGTGGAG CCTGTGCAAT
    GAAGAGGGCA TCCAGTATCG CAGCCGTTAC TGTGAGGTAC ACAGCCCAGA CTCTTCTCAG
    TGCGTTGGAA ACAGCACACA ATACCAAGAT TGCCTGTATA ATGAAATTCC TGTGATCCTG
    CCAGCATCCA GCATTGATGA GAGCACAAAC TGTGGAGGTT TTAGTCTCAT CCATCTGATT
    GCCACTGGGG TCTCCTGCTT CTTTGGCTCT AGCCTCTTAA CGTTTGTGAT TTACGTGTAC
    TGCCAGCGCT GCCAGAGGCA GTCCCAGGAG TCGACCGTTA TCCACCCAAC CACTCCCAAC
    CATCTTCATT ACAAGGGCAA CACAACCCCC AAGAATGAGA AGTACACCCC CATGGAGTTC
    AAGCTGACAG AAGTGACACA CTTAATATCG GGGGGTCCAA ACCTGGATAT CTACCAATGG
    AGATGGAGAT CAGACACTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa11314
    Clone ID Related Accession (Same CDS sequence) XM_004942927.2 , XM_025152596.1 , XM_015289990.2 , XM_015289989.1 , XM_015289990.1 , XM_004942927.3 , XM_015289989.2
    Accession Version XM_004942927.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3453bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product semaphorin-5B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004942927.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGTTGTTT CAAGATTAAA GGCCATTTCC CTGTCATTGC CAAGCCTTTT TCTTCTTGCT 
    TTTCATCTCT CTGCATCACA GAATGTACCT GAGTACTCTG AAGCTGAACA TCAGCAGTGT
    GTCAGGAAAG AACACCCCAC AATCGCTTTT GAAGATCTGA AGCCTTGGGT GTCCAATTTC
    ACCTATCCAG GTGTGCATGA TTTTTCTCAG CTCGCCCTGG ATGCCAACAG GAACCAGCTC
    ATTGTGGGAG CAAGGAACTA CCTCTTCAGA CTTAGTCTAC ACAACGTTTC TCTTATTCAG
    GCAACAGCAT GGGGTTCAGA TGAAGACACC AGGAGGTCCT GTCAAAGTAA AGGGAAAACA
    GAGGAGGAGT GCCAGAACTA TGTCCGAGTG CTGATTGTTT ATGGCAAGAA GGTTTTTACC
    TGTGGTACCA ATGCCTTTTC ACCAGTGTGT TCCAGCCGTC AGGTAGGAAA TCTTAGCAAA
    ATCATTGACA GAATTAATGG AGTGGCTCGG TGCCCATATG ACCCCCGGCA TAACTCAACA
    GCTGTGATTA CATCACGAGG AGAACTCTAT GCTGCAACTG TAATTGATTT CTCTGGACGG
    GATCCTGCTA TTTACCGCAG CCTTGGAAAT GTGCCCCCAC TCAGGACAGC ACAGTACAAC
    TCCAAATGGC TCAATGAGCC TAACTTTATC GCTGCCTATG ACATCGGTTT GTTCACCTAC
    TTCTTCTTTC GAGAGAATGC GGTAGAACAC GATTGTGGGA AAACAGTATA TTCTCGTGTG
    GCCAGGGTCT GCAAAAACGA CATTGGAGGG AGATTTTTGC TGGAGGACAC GTGGACAACT
    TTCATGAAAG CCAGGCTGAA CTGCTCCAGA GCTGGAGAAA TCCCTTTCTA TTATAATGAA
    CTGCAAAGCA CCTTCTACCT TCCTGAACAA GACCTGATCT ATGGTGTCTT CACTACTAAT
    GTAAACAGCA TAGCTGCCTC AGCAGTGTGT GCCTTCAATC TCAGTGCCAT TACTCAGGCA
    TTTAATGGTC CTTTCCGTTA CCAAGAGAAC CCAAGATCAG CCTGGCTGCC AACAGCAAAC
    CCCATCCCAA ATTTCCAGTG TGGGACACTG AGCGATGACA GCCCGAATGA GAACCTGACC
    GAGAGAGTCC TACAGGACGC GCAGCGCTTG TTCCTGATGA ACGACGTGGT GCAGCCGGTC
    ACTGTAGATC CGTATGTGAC TCAGGACAGC ATTCGATTCT CTAAACTAGT GGTTGATATT
    GTTCAGGGGA AGGATACTCT CTACCACGTG ATGTACATTG GAACAGAGTA TGGCACCATC
    TTGAAGGCCC TTTCTACCAC TAATAGGAGC CTGAGGAGTT GTTATTTAGA GGAAATGCAG
    ATCCTCCCTG ATGGACAACG GGAAGCAATC AAGAGTCTCC AAATCCTGCA CAGCGACAGA
    TCGCTCTTTG TGGGCTTAAA TAACGGAGTG CTGAAAATTC CTCTGGAGAG ATGCTCCATG
    TACAGAACAG AAGGGGAATG CCTAGGAGCC AGAGACCCTT ACTGTGGCTG GGATAACAAG
    CAGAAGCGAT GCACCACCAT TGAGGACAGC TCCAACATGA GCCTATGGAC TCAAAATATT
    ACTGAGTGCC CAGTGAAAAA CCTGACAACA AATGGAAGAC TGGGGCCCTG GTCCCCATGG
    CAACCGTGTG AGCATTCAGA TGGGGACAGC ACAGGCTCCT GTATGTGTAG GTCACGTTCG
    TGTGACAGCC CTCGTCCTCG CTGTGGAGGT CGCAGCTGTG AAGGGGCCAG GATCGAGGTC
    GCAAATTGTT CAAGAAATGG CGCTTGGACA CCCTGGTCTT CCTGGGCCCT GTGCAGCACC
    TCCTGTGGGA TAGGCTTTCA GGTCCGCCAG CGGTCCTGCA GCAACCCTGC TCCACGGTAT
    GGGGGCAGGG TCTGTGTTGG ACAGAGCAGG GAAGAGAGAT TCTGTAATGA GAATAGTCCA
    TGTCCATTGC CAATTTTTTG GTCTTCGTGG GGTCCCTGGA ATAAATGTAG TGTGAATTGT
    GGTGGAGGAA TTCACTCAAG GCAAAGGACC TGTGAAAATG GAAATACATG TCCAGGCTGT
    GCTGTGGAAT ATAAGACCTG CAACCCAGAG AGCTGCCCAG AAGTGCGTAG GAACACACCC
    TGGACCCCTT GGATGCCTGT CAACATCACC CAGAATGGTG CCCGCCAGGA GCAGCGCTTC
    CGCTATACGT GTCGTGCCCA GCTGTCTGAT CCCCATGAGC TGCAGTTTGG GAGGAAGAAA
    ACTGAGAGTC GATTCTGTCC CAATGATGGC TCAGCTATGT GTGAAACTGA CTCTCTTGTT
    GACGACCTCC TCAAGACTGG TAAGACCTCT GCACGGATTA TCAATGGGGG CTGGTCCTTT
    TGGGGGGCCT GGTCTTCCTG TTCCAGGGAC TGTGAGTTGG GCTTTAGAAT CCGGAAAAGA
    ACATGCACAA ACCCTGAACC TAAAAATGGT GGCTTGCCTT GCGTGGGGTC AGCGATGGAG
    TACCAAGACT GCAACCCACA TCCTTGCCCA GTAAAAGGCT CATGGTCTTG CTGGACTCCT
    TGGTCTCAGT GCTCAGCAAC CTGTGGAGGA GGGCATTACC AGCGCACACG GACTTGCACC
    AACCCAGCTC CATCCAGTGG AGAGGATATC TGCATCGGGC TGCACACTGA GGAGGCTCTC
    TGCAACACCC ACCCATGTGA AGGTGGCTGG TCAGAGTGGT CAGAGTGGAG CCTGTGCAAT
    GAAGAGGGCA TCCAGTATCG CAGCCGTTAC TGTGAGGTAC ACAGCCCAGA CTCTTCTCAG
    TGCGTTGGAA ACAGCACACA ATACCAAGAT TGCCTGTATA ATGAAATTCC TGTGATCCTG
    CCAGCATCCA GCATTGATGA GAGCACAAAC TGTGGAGGTT TTAGTCTCAT CCATCTGATT
    GCCACTGGGG TCTCCTGCTT CTTTGGCTCT AGCCTCTTAA CGTTTGTGAT TTACGTGTAC
    TGCCAGCGCT GCCAGAGGCA GTCCCAGGAG TCGACCGTTA TCCACCCAAC CACTCCCAAC
    CATCTTCATT ACAAGGGCAA CACAACCCCC AAGAATGAGA AGTACACCCC CATGGAGTTC
    AAGACAGGAG ATGCGCTCAT ACCTTCCCTA AACGTGCTGT GCCTCGATCA CGAGGATGCT
    GTAGCAAACA AAGCCTGTGT TGACCCTCAA ATTCAAGCAG TGAGTTCCCT ACTGTACTGC
    ATGGCAAACA TTAAGGATGT GTGTAGGAGC ATTATAGGCC AGAGACAAGA AGTTGAAGCC
    AGGGACTCGT TGAAAAATGT TGTTAGAAAT GTGCGTCAAC CAAAGTTGTA TACTGTAAAG
    ATGAAGGAGA AAGCTTCATA CAATCATTGC TTCTTCCAAA ATGGGATTTT ATCCTTTTGC
    ATAGGAAAAG CCTGGCATAG CAATGGGTTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004942984.1
    CDS608..4060
    Translation

    Target ORF information:

    RefSeq Version XM_004942927.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (SEMA5B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004942927.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGTTGTTT CAAGATTAAA GGCCATTTCC CTGTCATTGC CAAGCCTTTT TCTTCTTGCT 
    TTTCATCTCT CTGCATCACA GAATGTACCT GAGTACTCTG AAGCTGAACA TCAGCAGTGT
    GTCAGGAAAG AACACCCCAC AATCGCTTTT GAAGATCTGA AGCCTTGGGT GTCCAATTTC
    ACCTATCCAG GTGTGCATGA TTTTTCTCAG CTCGCCCTGG ATGCCAACAG GAACCAGCTC
    ATTGTGGGAG CAAGGAACTA CCTCTTCAGA CTTAGTCTAC ACAACGTTTC TCTTATTCAG
    GCAACAGCAT GGGGTTCAGA TGAAGACACC AGGAGGTCCT GTCAAAGTAA AGGGAAAACA
    GAGGAGGAGT GCCAGAACTA TGTCCGAGTG CTGATTGTTT ATGGCAAGAA GGTTTTTACC
    TGTGGTACCA ATGCCTTTTC ACCAGTGTGT TCCAGCCGTC AGGTAGGAAA TCTTAGCAAA
    ATCATTGACA GAATTAATGG AGTGGCTCGG TGCCCATATG ACCCCCGGCA TAACTCAACA
    GCTGTGATTA CATCACGAGG AGAACTCTAT GCTGCAACTG TAATTGATTT CTCTGGACGG
    GATCCTGCTA TTTACCGCAG CCTTGGAAAT GTGCCCCCAC TCAGGACAGC ACAGTACAAC
    TCCAAATGGC TCAATGAGCC TAACTTTATC GCTGCCTATG ACATCGGTTT GTTCACCTAC
    TTCTTCTTTC GAGAGAATGC GGTAGAACAC GATTGTGGGA AAACAGTATA TTCTCGTGTG
    GCCAGGGTCT GCAAAAACGA CATTGGAGGG AGATTTTTGC TGGAGGACAC GTGGACAACT
    TTCATGAAAG CCAGGCTGAA CTGCTCCAGA GCTGGAGAAA TCCCTTTCTA TTATAATGAA
    CTGCAAAGCA CCTTCTACCT TCCTGAACAA GACCTGATCT ATGGTGTCTT CACTACTAAT
    GTAAACAGCA TAGCTGCCTC AGCAGTGTGT GCCTTCAATC TCAGTGCCAT TACTCAGGCA
    TTTAATGGTC CTTTCCGTTA CCAAGAGAAC CCAAGATCAG CCTGGCTGCC AACAGCAAAC
    CCCATCCCAA ATTTCCAGTG TGGGACACTG AGCGATGACA GCCCGAATGA GAACCTGACC
    GAGAGAGTCC TACAGGACGC GCAGCGCTTG TTCCTGATGA ACGACGTGGT GCAGCCGGTC
    ACTGTAGATC CGTATGTGAC TCAGGACAGC ATTCGATTCT CTAAACTAGT GGTTGATATT
    GTTCAGGGGA AGGATACTCT CTACCACGTG ATGTACATTG GAACAGAGTA TGGCACCATC
    TTGAAGGCCC TTTCTACCAC TAATAGGAGC CTGAGGAGTT GTTATTTAGA GGAAATGCAG
    ATCCTCCCTG ATGGACAACG GGAAGCAATC AAGAGTCTCC AAATCCTGCA CAGCGACAGA
    TCGCTCTTTG TGGGCTTAAA TAACGGAGTG CTGAAAATTC CTCTGGAGAG ATGCTCCATG
    TACAGAACAG AAGGGGAATG CCTAGGAGCC AGAGACCCTT ACTGTGGCTG GGATAACAAG
    CAGAAGCGAT GCACCACCAT TGAGGACAGC TCCAACATGA GCCTATGGAC TCAAAATATT
    ACTGAGTGCC CAGTGAAAAA CCTGACAACA AATGGAAGAC TGGGGCCCTG GTCCCCATGG
    CAACCGTGTG AGCATTCAGA TGGGGACAGC ACAGGCTCCT GTATGTGTAG GTCACGTTCG
    TGTGACAGCC CTCGTCCTCG CTGTGGAGGT CGCAGCTGTG AAGGGGCCAG GATCGAGGTC
    GCAAATTGTT CAAGAAATGG CGCTTGGACA CCCTGGTCTT CCTGGGCCCT GTGCAGCACC
    TCCTGTGGGA TAGGCTTTCA GGTCCGCCAG CGGTCCTGCA GCAACCCTGC TCCACGGTAT
    GGGGGCAGGG TCTGTGTTGG ACAGAGCAGG GAAGAGAGAT TCTGTAATGA GAATAGTCCA
    TGTCCATTGC CAATTTTTTG GTCTTCGTGG GGTCCCTGGA ATAAATGTAG TGTGAATTGT
    GGTGGAGGAA TTCACTCAAG GCAAAGGACC TGTGAAAATG GAAATACATG TCCAGGCTGT
    GCTGTGGAAT ATAAGACCTG CAACCCAGAG AGCTGCCCAG AAGTGCGTAG GAACACACCC
    TGGACCCCTT GGATGCCTGT CAACATCACC CAGAATGGTG CCCGCCAGGA GCAGCGCTTC
    CGCTATACGT GTCGTGCCCA GCTGTCTGAT CCCCATGAGC TGCAGTTTGG GAGGAAGAAA
    ACTGAGAGTC GATTCTGTCC CAATGATGGC TCAGCTATGT GTGAAACTGA CTCTCTTGTT
    GACGACCTCC TCAAGACTGG TAAGACCTCT GCACGGATTA TCAATGGGGG CTGGTCCTTT
    TGGGGGGCCT GGTCTTCCTG TTCCAGGGAC TGTGAGTTGG GCTTTAGAAT CCGGAAAAGA
    ACATGCACAA ACCCTGAACC TAAAAATGGT GGCTTGCCTT GCGTGGGGTC AGCGATGGAG
    TACCAAGACT GCAACCCACA TCCTTGCCCA GTAAAAGGCT CATGGTCTTG CTGGACTCCT
    TGGTCTCAGT GCTCAGCAAC CTGTGGAGGA GGGCATTACC AGCGCACACG GACTTGCACC
    AACCCAGCTC CATCCAGTGG AGAGGATATC TGCATCGGGC TGCACACTGA GGAGGCTCTC
    TGCAACACCC ACCCATGTGA AGGTGGCTGG TCAGAGTGGT CAGAGTGGAG CCTGTGCAAT
    GAAGAGGGCA TCCAGTATCG CAGCCGTTAC TGTGAGGTAC ACAGCCCAGA CTCTTCTCAG
    TGCGTTGGAA ACAGCACACA ATACCAAGAT TGCCTGTATA ATGAAATTCC TGTGATCCTG
    CCAGCATCCA GCATTGATGA GAGCACAAAC TGTGGAGGTT TTAGTCTCAT CCATCTGATT
    GCCACTGGGG TCTCCTGCTT CTTTGGCTCT AGCCTCTTAA CGTTTGTGAT TTACGTGTAC
    TGCCAGCGCT GCCAGAGGCA GTCCCAGGAG TCGACCGTTA TCCACCCAAC CACTCCCAAC
    CATCTTCATT ACAAGGGCAA CACAACCCCC AAGAATGAGA AGTACACCCC CATGGAGTTC
    AAGACAGGAG ATGCGCTCAT ACCTTCCCTA AACGTGCTGT GCCTCGATCA CGAGGATGCT
    GTAGCAAACA AAGCCTGTGT TGACCCTCAA ATTCAAGCAG TGAGTTCCCT ACTGTACTGC
    ATGGCAAACA TTAAGGATGT GTGTAGGAGC ATTATAGGCC AGAGACAAGA AGTTGAAGCC
    AGGGACTCGT TGAAAAATGT TGTTAGAAAT GTGCGTCAAC CAAAGTTGTA TACTGTAAAG
    ATGAAGGAGA AAGCTTCATA CAATCATTGC TTCTTCCAAA ATGGGATTTT ATCCTTTTGC
    ATAGGAAAAG CCTGGCATAG CAATGGGTTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa11314
    Clone ID Related Accession (Same CDS sequence) XM_004942927.2 , XM_025152596.1 , XM_015289990.2 , XM_015289989.1 , XM_015289990.1 , XM_004942927.3 , XM_015289989.2
    Accession Version XM_025152596.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3453bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product semaphorin-5B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGTTGTTT CAAGATTAAA GGCCATTTCC CTGTCATTGC CAAGCCTTTT TCTTCTTGCT 
    TTTCATCTCT CTGCATCACA GAATGTACCT GAGTACTCTG AAGCTGAACA TCAGCAGTGT
    GTCAGGAAAG AACACCCCAC AATCGCTTTT GAAGATCTGA AGCCTTGGGT GTCCAATTTC
    ACCTATCCAG GTGTGCATGA TTTTTCTCAG CTCGCCCTGG ATGCCAACAG GAACCAGCTC
    ATTGTGGGAG CAAGGAACTA CCTCTTCAGA CTTAGTCTAC ACAACGTTTC TCTTATTCAG
    GCAACAGCAT GGGGTTCAGA TGAAGACACC AGGAGGTCCT GTCAAAGTAA AGGGAAAACA
    GAGGAGGAGT GCCAGAACTA TGTCCGAGTG CTGATTGTTT ATGGCAAGAA GGTTTTTACC
    TGTGGTACCA ATGCCTTTTC ACCAGTGTGT TCCAGCCGTC AGGTAGGAAA TCTTAGCAAA
    ATCATTGACA GAATTAATGG AGTGGCTCGG TGCCCATATG ACCCCCGGCA TAACTCAACA
    GCTGTGATTA CATCACGAGG AGAACTCTAT GCTGCAACTG TAATTGATTT CTCTGGACGG
    GATCCTGCTA TTTACCGCAG CCTTGGAAAT GTGCCCCCAC TCAGGACAGC ACAGTACAAC
    TCCAAATGGC TCAATGAGCC TAACTTTATC GCTGCCTATG ACATCGGTTT GTTCACCTAC
    TTCTTCTTTC GAGAGAATGC GGTAGAACAC GATTGTGGGA AAACAGTATA TTCTCGTGTG
    GCCAGGGTCT GCAAAAACGA CATTGGAGGG AGATTTTTGC TGGAGGACAC GTGGACAACT
    TTCATGAAAG CCAGGCTGAA CTGCTCCAGA GCTGGAGAAA TCCCTTTCTA TTATAATGAA
    CTGCAAAGCA CCTTCTACCT TCCTGAACAA GACCTGATCT ATGGTGTCTT CACTACTAAT
    GTAAACAGCA TAGCTGCCTC AGCAGTGTGT GCCTTCAATC TCAGTGCCAT TACTCAGGCA
    TTTAATGGTC CTTTCCGTTA CCAAGAGAAC CCAAGATCAG CCTGGCTGCC AACAGCAAAC
    CCCATCCCAA ATTTCCAGTG TGGGACACTG AGCGATGACA GCCCGAATGA GAACCTGACC
    GAGAGAGTCC TACAGGACGC GCAGCGCTTG TTCCTGATGA ACGACGTGGT GCAGCCGGTC
    ACTGTAGATC CGTATGTGAC TCAGGACAGC ATTCGATTCT CTAAACTAGT GGTTGATATT
    GTTCAGGGGA AGGATACTCT CTACCACGTG ATGTACATTG GAACAGAGTA TGGCACCATC
    TTGAAGGCCC TTTCTACCAC TAATAGGAGC CTGAGGAGTT GTTATTTAGA GGAAATGCAG
    ATCCTCCCTG ATGGACAACG GGAAGCAATC AAGAGTCTCC AAATCCTGCA CAGCGACAGA
    TCGCTCTTTG TGGGCTTAAA TAACGGAGTG CTGAAAATTC CTCTGGAGAG ATGCTCCATG
    TACAGAACAG AAGGGGAATG CCTAGGAGCC AGAGACCCTT ACTGTGGCTG GGATAACAAG
    CAGAAGCGAT GCACCACCAT TGAGGACAGC TCCAACATGA GCCTATGGAC TCAAAATATT
    ACTGAGTGCC CAGTGAAAAA CCTGACAACA AATGGAAGAC TGGGGCCCTG GTCCCCATGG
    CAACCGTGTG AGCATTCAGA TGGGGACAGC ACAGGCTCCT GTATGTGTAG GTCACGTTCG
    TGTGACAGCC CTCGTCCTCG CTGTGGAGGT CGCAGCTGTG AAGGGGCCAG GATCGAGGTC
    GCAAATTGTT CAAGAAATGG CGCTTGGACA CCCTGGTCTT CCTGGGCCCT GTGCAGCACC
    TCCTGTGGGA TAGGCTTTCA GGTCCGCCAG CGGTCCTGCA GCAACCCTGC TCCACGGTAT
    GGGGGCAGGG TCTGTGTTGG ACAGAGCAGG GAAGAGAGAT TCTGTAATGA GAATAGTCCA
    TGTCCATTGC CAATTTTTTG GTCTTCGTGG GGTCCCTGGA ATAAATGTAG TGTGAATTGT
    GGTGGAGGAA TTCACTCAAG GCAAAGGACC TGTGAAAATG GAAATACATG TCCAGGCTGT
    GCTGTGGAAT ATAAGACCTG CAACCCAGAG AGCTGCCCAG AAGTGCGTAG GAACACACCC
    TGGACCCCTT GGATGCCTGT CAACATCACC CAGAATGGTG CCCGCCAGGA GCAGCGCTTC
    CGCTATACGT GTCGTGCCCA GCTGTCTGAT CCCCATGAGC TGCAGTTTGG GAGGAAGAAA
    ACTGAGAGTC GATTCTGTCC CAATGATGGC TCAGCTATGT GTGAAACTGA CTCTCTTGTT
    GACGACCTCC TCAAGACTGG TAAGACCTCT GCACGGATTA TCAATGGGGG CTGGTCCTTT
    TGGGGGGCCT GGTCTTCCTG TTCCAGGGAC TGTGAGTTGG GCTTTAGAAT CCGGAAAAGA
    ACATGCACAA ACCCTGAACC TAAAAATGGT GGCTTGCCTT GCGTGGGGTC AGCGATGGAG
    TACCAAGACT GCAACCCACA TCCTTGCCCA GTAAAAGGCT CATGGTCTTG CTGGACTCCT
    TGGTCTCAGT GCTCAGCAAC CTGTGGAGGA GGGCATTACC AGCGCACACG GACTTGCACC
    AACCCAGCTC CATCCAGTGG AGAGGATATC TGCATCGGGC TGCACACTGA GGAGGCTCTC
    TGCAACACCC ACCCATGTGA AGGTGGCTGG TCAGAGTGGT CAGAGTGGAG CCTGTGCAAT
    GAAGAGGGCA TCCAGTATCG CAGCCGTTAC TGTGAGGTAC ACAGCCCAGA CTCTTCTCAG
    TGCGTTGGAA ACAGCACACA ATACCAAGAT TGCCTGTATA ATGAAATTCC TGTGATCCTG
    CCAGCATCCA GCATTGATGA GAGCACAAAC TGTGGAGGTT TTAGTCTCAT CCATCTGATT
    GCCACTGGGG TCTCCTGCTT CTTTGGCTCT AGCCTCTTAA CGTTTGTGAT TTACGTGTAC
    TGCCAGCGCT GCCAGAGGCA GTCCCAGGAG TCGACCGTTA TCCACCCAAC CACTCCCAAC
    CATCTTCATT ACAAGGGCAA CACAACCCCC AAGAATGAGA AGTACACCCC CATGGAGTTC
    AAGACAGGAG ATGCGCTCAT ACCTTCCCTA AACGTGCTGT GCCTCGATCA CGAGGATGCT
    GTAGCAAACA AAGCCTGTGT TGACCCTCAA ATTCAAGCAG TGAGTTCCCT ACTGTACTGC
    ATGGCAAACA TTAAGGATGT GTGTAGGAGC ATTATAGGCC AGAGACAAGA AGTTGAAGCC
    AGGGACTCGT TGAAAAATGT TGTTAGAAAT GTGCGTCAAC CAAAGTTGTA TACTGTAAAG
    ATGAAGGAGA AAGCTTCATA CAATCATTGC TTCTTCCAAA ATGGGATTTT ATCCTTTTGC
    ATAGGAAAAG CCTGGCATAG CAATGGGTTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025008364.1
    CDS179..3631
    Translation

    Target ORF information:

    RefSeq Version XM_025152596.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus semaphorin 5B (SEMA5B), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025152596.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGTTGTTT CAAGATTAAA GGCCATTTCC CTGTCATTGC CAAGCCTTTT TCTTCTTGCT 
    TTTCATCTCT CTGCATCACA GAATGTACCT GAGTACTCTG AAGCTGAACA TCAGCAGTGT
    GTCAGGAAAG AACACCCCAC AATCGCTTTT GAAGATCTGA AGCCTTGGGT GTCCAATTTC
    ACCTATCCAG GTGTGCATGA TTTTTCTCAG CTCGCCCTGG ATGCCAACAG GAACCAGCTC
    ATTGTGGGAG CAAGGAACTA CCTCTTCAGA CTTAGTCTAC ACAACGTTTC TCTTATTCAG
    GCAACAGCAT GGGGTTCAGA TGAAGACACC AGGAGGTCCT GTCAAAGTAA AGGGAAAACA
    GAGGAGGAGT GCCAGAACTA TGTCCGAGTG CTGATTGTTT ATGGCAAGAA GGTTTTTACC
    TGTGGTACCA ATGCCTTTTC ACCAGTGTGT TCCAGCCGTC AGGTAGGAAA TCTTAGCAAA
    ATCATTGACA GAATTAATGG AGTGGCTCGG TGCCCATATG ACCCCCGGCA TAACTCAACA
    GCTGTGATTA CATCACGAGG AGAACTCTAT GCTGCAACTG TAATTGATTT CTCTGGACGG
    GATCCTGCTA TTTACCGCAG CCTTGGAAAT GTGCCCCCAC TCAGGACAGC ACAGTACAAC
    TCCAAATGGC TCAATGAGCC TAACTTTATC GCTGCCTATG ACATCGGTTT GTTCACCTAC
    TTCTTCTTTC GAGAGAATGC GGTAGAACAC GATTGTGGGA AAACAGTATA TTCTCGTGTG
    GCCAGGGTCT GCAAAAACGA CATTGGAGGG AGATTTTTGC TGGAGGACAC GTGGACAACT
    TTCATGAAAG CCAGGCTGAA CTGCTCCAGA GCTGGAGAAA TCCCTTTCTA TTATAATGAA
    CTGCAAAGCA CCTTCTACCT TCCTGAACAA GACCTGATCT ATGGTGTCTT CACTACTAAT
    GTAAACAGCA TAGCTGCCTC AGCAGTGTGT GCCTTCAATC TCAGTGCCAT TACTCAGGCA
    TTTAATGGTC CTTTCCGTTA CCAAGAGAAC CCAAGATCAG CCTGGCTGCC AACAGCAAAC
    CCCATCCCAA ATTTCCAGTG TGGGACACTG AGCGATGACA GCCCGAATGA GAACCTGACC
    GAGAGAGTCC TACAGGACGC GCAGCGCTTG TTCCTGATGA ACGACGTGGT GCAGCCGGTC
    ACTGTAGATC CGTATGTGAC TCAGGACAGC ATTCGATTCT CTAAACTAGT GGTTGATATT
    GTTCAGGGGA AGGATACTCT CTACCACGTG ATGTACATTG GAACAGAGTA TGGCACCATC
    TTGAAGGCCC TTTCTACCAC TAATAGGAGC CTGAGGAGTT GTTATTTAGA GGAAATGCAG
    ATCCTCCCTG ATGGACAACG GGAAGCAATC AAGAGTCTCC AAATCCTGCA CAGCGACAGA
    TCGCTCTTTG TGGGCTTAAA TAACGGAGTG CTGAAAATTC CTCTGGAGAG ATGCTCCATG
    TACAGAACAG AAGGGGAATG CCTAGGAGCC AGAGACCCTT ACTGTGGCTG GGATAACAAG
    CAGAAGCGAT GCACCACCAT TGAGGACAGC TCCAACATGA GCCTATGGAC TCAAAATATT
    ACTGAGTGCC CAGTGAAAAA CCTGACAACA AATGGAAGAC TGGGGCCCTG GTCCCCATGG
    CAACCGTGTG AGCATTCAGA TGGGGACAGC ACAGGCTCCT GTATGTGTAG GTCACGTTCG
    TGTGACAGCC CTCGTCCTCG CTGTGGAGGT CGCAGCTGTG AAGGGGCCAG GATCGAGGTC
    GCAAATTGTT CAAGAAATGG CGCTTGGACA CCCTGGTCTT CCTGGGCCCT GTGCAGCACC
    TCCTGTGGGA TAGGCTTTCA GGTCCGCCAG CGGTCCTGCA GCAACCCTGC TCCACGGTAT
    GGGGGCAGGG TCTGTGTTGG ACAGAGCAGG GAAGAGAGAT TCTGTAATGA GAATAGTCCA
    TGTCCATTGC CAATTTTTTG GTCTTCGTGG GGTCCCTGGA ATAAATGTAG TGTGAATTGT
    GGTGGAGGAA TTCACTCAAG GCAAAGGACC TGTGAAAATG GAAATACATG TCCAGGCTGT
    GCTGTGGAAT ATAAGACCTG CAACCCAGAG AGCTGCCCAG AAGTGCGTAG GAACACACCC
    TGGACCCCTT GGATGCCTGT CAACATCACC CAGAATGGTG CCCGCCAGGA GCAGCGCTTC
    CGCTATACGT GTCGTGCCCA GCTGTCTGAT CCCCATGAGC TGCAGTTTGG GAGGAAGAAA
    ACTGAGAGTC GATTCTGTCC CAATGATGGC TCAGCTATGT GTGAAACTGA CTCTCTTGTT
    GACGACCTCC TCAAGACTGG TAAGACCTCT GCACGGATTA TCAATGGGGG CTGGTCCTTT
    TGGGGGGCCT GGTCTTCCTG TTCCAGGGAC TGTGAGTTGG GCTTTAGAAT CCGGAAAAGA
    ACATGCACAA ACCCTGAACC TAAAAATGGT GGCTTGCCTT GCGTGGGGTC AGCGATGGAG
    TACCAAGACT GCAACCCACA TCCTTGCCCA GTAAAAGGCT CATGGTCTTG CTGGACTCCT
    TGGTCTCAGT GCTCAGCAAC CTGTGGAGGA GGGCATTACC AGCGCACACG GACTTGCACC
    AACCCAGCTC CATCCAGTGG AGAGGATATC TGCATCGGGC TGCACACTGA GGAGGCTCTC
    TGCAACACCC ACCCATGTGA AGGTGGCTGG TCAGAGTGGT CAGAGTGGAG CCTGTGCAAT
    GAAGAGGGCA TCCAGTATCG CAGCCGTTAC TGTGAGGTAC ACAGCCCAGA CTCTTCTCAG
    TGCGTTGGAA ACAGCACACA ATACCAAGAT TGCCTGTATA ATGAAATTCC TGTGATCCTG
    CCAGCATCCA GCATTGATGA GAGCACAAAC TGTGGAGGTT TTAGTCTCAT CCATCTGATT
    GCCACTGGGG TCTCCTGCTT CTTTGGCTCT AGCCTCTTAA CGTTTGTGAT TTACGTGTAC
    TGCCAGCGCT GCCAGAGGCA GTCCCAGGAG TCGACCGTTA TCCACCCAAC CACTCCCAAC
    CATCTTCATT ACAAGGGCAA CACAACCCCC AAGAATGAGA AGTACACCCC CATGGAGTTC
    AAGACAGGAG ATGCGCTCAT ACCTTCCCTA AACGTGCTGT GCCTCGATCA CGAGGATGCT
    GTAGCAAACA AAGCCTGTGT TGACCCTCAA ATTCAAGCAG TGAGTTCCCT ACTGTACTGC
    ATGGCAAACA TTAAGGATGT GTGTAGGAGC ATTATAGGCC AGAGACAAGA AGTTGAAGCC
    AGGGACTCGT TGAAAAATGT TGTTAGAAAT GTGCGTCAAC CAAAGTTGTA TACTGTAAAG
    ATGAAGGAGA AAGCTTCATA CAATCATTGC TTCTTCCAAA ATGGGATTTT ATCCTTTTGC
    ATAGGAAAAG CCTGGCATAG CAATGGGTTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa11314
    Clone ID Related Accession (Same CDS sequence) XM_004942927.2 , XM_025152596.1 , XM_015289990.2 , XM_015289989.1 , XM_015289990.1 , XM_004942927.3 , XM_015289989.2
    Accession Version XM_015289990.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3453bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product semaphorin-5B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289990.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGTTGTTT CAAGATTAAA GGCCATTTCC CTGTCATTGC CAAGCCTTTT TCTTCTTGCT 
    TTTCATCTCT CTGCATCACA GAATGTACCT GAGTACTCTG AAGCTGAACA TCAGCAGTGT
    GTCAGGAAAG AACACCCCAC AATCGCTTTT GAAGATCTGA AGCCTTGGGT GTCCAATTTC
    ACCTATCCAG GTGTGCATGA TTTTTCTCAG CTCGCCCTGG ATGCCAACAG GAACCAGCTC
    ATTGTGGGAG CAAGGAACTA CCTCTTCAGA CTTAGTCTAC ACAACGTTTC TCTTATTCAG
    GCAACAGCAT GGGGTTCAGA TGAAGACACC AGGAGGTCCT GTCAAAGTAA AGGGAAAACA
    GAGGAGGAGT GCCAGAACTA TGTCCGAGTG CTGATTGTTT ATGGCAAGAA GGTTTTTACC
    TGTGGTACCA ATGCCTTTTC ACCAGTGTGT TCCAGCCGTC AGGTAGGAAA TCTTAGCAAA
    ATCATTGACA GAATTAATGG AGTGGCTCGG TGCCCATATG ACCCCCGGCA TAACTCAACA
    GCTGTGATTA CATCACGAGG AGAACTCTAT GCTGCAACTG TAATTGATTT CTCTGGACGG
    GATCCTGCTA TTTACCGCAG CCTTGGAAAT GTGCCCCCAC TCAGGACAGC ACAGTACAAC
    TCCAAATGGC TCAATGAGCC TAACTTTATC GCTGCCTATG ACATCGGTTT GTTCACCTAC
    TTCTTCTTTC GAGAGAATGC GGTAGAACAC GATTGTGGGA AAACAGTATA TTCTCGTGTG
    GCCAGGGTCT GCAAAAACGA CATTGGAGGG AGATTTTTGC TGGAGGACAC GTGGACAACT
    TTCATGAAAG CCAGGCTGAA CTGCTCCAGA GCTGGAGAAA TCCCTTTCTA TTATAATGAA
    CTGCAAAGCA CCTTCTACCT TCCTGAACAA GACCTGATCT ATGGTGTCTT CACTACTAAT
    GTAAACAGCA TAGCTGCCTC AGCAGTGTGT GCCTTCAATC TCAGTGCCAT TACTCAGGCA
    TTTAATGGTC CTTTCCGTTA CCAAGAGAAC CCAAGATCAG CCTGGCTGCC AACAGCAAAC
    CCCATCCCAA ATTTCCAGTG TGGGACACTG AGCGATGACA GCCCGAATGA GAACCTGACC
    GAGAGAGTCC TACAGGACGC GCAGCGCTTG TTCCTGATGA ACGACGTGGT GCAGCCGGTC
    ACTGTAGATC CGTATGTGAC TCAGGACAGC ATTCGATTCT CTAAACTAGT GGTTGATATT
    GTTCAGGGGA AGGATACTCT CTACCACGTG ATGTACATTG GAACAGAGTA TGGCACCATC
    TTGAAGGCCC TTTCTACCAC TAATAGGAGC CTGAGGAGTT GTTATTTAGA GGAAATGCAG
    ATCCTCCCTG ATGGACAACG GGAAGCAATC AAGAGTCTCC AAATCCTGCA CAGCGACAGA
    TCGCTCTTTG TGGGCTTAAA TAACGGAGTG CTGAAAATTC CTCTGGAGAG ATGCTCCATG
    TACAGAACAG AAGGGGAATG CCTAGGAGCC AGAGACCCTT ACTGTGGCTG GGATAACAAG
    CAGAAGCGAT GCACCACCAT TGAGGACAGC TCCAACATGA GCCTATGGAC TCAAAATATT
    ACTGAGTGCC CAGTGAAAAA CCTGACAACA AATGGAAGAC TGGGGCCCTG GTCCCCATGG
    CAACCGTGTG AGCATTCAGA TGGGGACAGC ACAGGCTCCT GTATGTGTAG GTCACGTTCG
    TGTGACAGCC CTCGTCCTCG CTGTGGAGGT CGCAGCTGTG AAGGGGCCAG GATCGAGGTC
    GCAAATTGTT CAAGAAATGG CGCTTGGACA CCCTGGTCTT CCTGGGCCCT GTGCAGCACC
    TCCTGTGGGA TAGGCTTTCA GGTCCGCCAG CGGTCCTGCA GCAACCCTGC TCCACGGTAT
    GGGGGCAGGG TCTGTGTTGG ACAGAGCAGG GAAGAGAGAT TCTGTAATGA GAATAGTCCA
    TGTCCATTGC CAATTTTTTG GTCTTCGTGG GGTCCCTGGA ATAAATGTAG TGTGAATTGT
    GGTGGAGGAA TTCACTCAAG GCAAAGGACC TGTGAAAATG GAAATACATG TCCAGGCTGT
    GCTGTGGAAT ATAAGACCTG CAACCCAGAG AGCTGCCCAG AAGTGCGTAG GAACACACCC
    TGGACCCCTT GGATGCCTGT CAACATCACC CAGAATGGTG CCCGCCAGGA GCAGCGCTTC
    CGCTATACGT GTCGTGCCCA GCTGTCTGAT CCCCATGAGC TGCAGTTTGG GAGGAAGAAA
    ACTGAGAGTC GATTCTGTCC CAATGATGGC TCAGCTATGT GTGAAACTGA CTCTCTTGTT
    GACGACCTCC TCAAGACTGG TAAGACCTCT GCACGGATTA TCAATGGGGG CTGGTCCTTT
    TGGGGGGCCT GGTCTTCCTG TTCCAGGGAC TGTGAGTTGG GCTTTAGAAT CCGGAAAAGA
    ACATGCACAA ACCCTGAACC TAAAAATGGT GGCTTGCCTT GCGTGGGGTC AGCGATGGAG
    TACCAAGACT GCAACCCACA TCCTTGCCCA GTAAAAGGCT CATGGTCTTG CTGGACTCCT
    TGGTCTCAGT GCTCAGCAAC CTGTGGAGGA GGGCATTACC AGCGCACACG GACTTGCACC
    AACCCAGCTC CATCCAGTGG AGAGGATATC TGCATCGGGC TGCACACTGA GGAGGCTCTC
    TGCAACACCC ACCCATGTGA AGGTGGCTGG TCAGAGTGGT CAGAGTGGAG CCTGTGCAAT
    GAAGAGGGCA TCCAGTATCG CAGCCGTTAC TGTGAGGTAC ACAGCCCAGA CTCTTCTCAG
    TGCGTTGGAA ACAGCACACA ATACCAAGAT TGCCTGTATA ATGAAATTCC TGTGATCCTG
    CCAGCATCCA GCATTGATGA GAGCACAAAC TGTGGAGGTT TTAGTCTCAT CCATCTGATT
    GCCACTGGGG TCTCCTGCTT CTTTGGCTCT AGCCTCTTAA CGTTTGTGAT TTACGTGTAC
    TGCCAGCGCT GCCAGAGGCA GTCCCAGGAG TCGACCGTTA TCCACCCAAC CACTCCCAAC
    CATCTTCATT ACAAGGGCAA CACAACCCCC AAGAATGAGA AGTACACCCC CATGGAGTTC
    AAGACAGGAG ATGCGCTCAT ACCTTCCCTA AACGTGCTGT GCCTCGATCA CGAGGATGCT
    GTAGCAAACA AAGCCTGTGT TGACCCTCAA ATTCAAGCAG TGAGTTCCCT ACTGTACTGC
    ATGGCAAACA TTAAGGATGT GTGTAGGAGC ATTATAGGCC AGAGACAAGA AGTTGAAGCC
    AGGGACTCGT TGAAAAATGT TGTTAGAAAT GTGCGTCAAC CAAAGTTGTA TACTGTAAAG
    ATGAAGGAGA AAGCTTCATA CAATCATTGC TTCTTCCAAA ATGGGATTTT ATCCTTTTGC
    ATAGGAAAAG CCTGGCATAG CAATGGGTTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145476.1
    CDS382..3834
    Translation

    Target ORF information:

    RefSeq Version XM_015289990.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus semaphorin 5B (SEMA5B), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289990.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGTTGTTT CAAGATTAAA GGCCATTTCC CTGTCATTGC CAAGCCTTTT TCTTCTTGCT 
    TTTCATCTCT CTGCATCACA GAATGTACCT GAGTACTCTG AAGCTGAACA TCAGCAGTGT
    GTCAGGAAAG AACACCCCAC AATCGCTTTT GAAGATCTGA AGCCTTGGGT GTCCAATTTC
    ACCTATCCAG GTGTGCATGA TTTTTCTCAG CTCGCCCTGG ATGCCAACAG GAACCAGCTC
    ATTGTGGGAG CAAGGAACTA CCTCTTCAGA CTTAGTCTAC ACAACGTTTC TCTTATTCAG
    GCAACAGCAT GGGGTTCAGA TGAAGACACC AGGAGGTCCT GTCAAAGTAA AGGGAAAACA
    GAGGAGGAGT GCCAGAACTA TGTCCGAGTG CTGATTGTTT ATGGCAAGAA GGTTTTTACC
    TGTGGTACCA ATGCCTTTTC ACCAGTGTGT TCCAGCCGTC AGGTAGGAAA TCTTAGCAAA
    ATCATTGACA GAATTAATGG AGTGGCTCGG TGCCCATATG ACCCCCGGCA TAACTCAACA
    GCTGTGATTA CATCACGAGG AGAACTCTAT GCTGCAACTG TAATTGATTT CTCTGGACGG
    GATCCTGCTA TTTACCGCAG CCTTGGAAAT GTGCCCCCAC TCAGGACAGC ACAGTACAAC
    TCCAAATGGC TCAATGAGCC TAACTTTATC GCTGCCTATG ACATCGGTTT GTTCACCTAC
    TTCTTCTTTC GAGAGAATGC GGTAGAACAC GATTGTGGGA AAACAGTATA TTCTCGTGTG
    GCCAGGGTCT GCAAAAACGA CATTGGAGGG AGATTTTTGC TGGAGGACAC GTGGACAACT
    TTCATGAAAG CCAGGCTGAA CTGCTCCAGA GCTGGAGAAA TCCCTTTCTA TTATAATGAA
    CTGCAAAGCA CCTTCTACCT TCCTGAACAA GACCTGATCT ATGGTGTCTT CACTACTAAT
    GTAAACAGCA TAGCTGCCTC AGCAGTGTGT GCCTTCAATC TCAGTGCCAT TACTCAGGCA
    TTTAATGGTC CTTTCCGTTA CCAAGAGAAC CCAAGATCAG CCTGGCTGCC AACAGCAAAC
    CCCATCCCAA ATTTCCAGTG TGGGACACTG AGCGATGACA GCCCGAATGA GAACCTGACC
    GAGAGAGTCC TACAGGACGC GCAGCGCTTG TTCCTGATGA ACGACGTGGT GCAGCCGGTC
    ACTGTAGATC CGTATGTGAC TCAGGACAGC ATTCGATTCT CTAAACTAGT GGTTGATATT
    GTTCAGGGGA AGGATACTCT CTACCACGTG ATGTACATTG GAACAGAGTA TGGCACCATC
    TTGAAGGCCC TTTCTACCAC TAATAGGAGC CTGAGGAGTT GTTATTTAGA GGAAATGCAG
    ATCCTCCCTG ATGGACAACG GGAAGCAATC AAGAGTCTCC AAATCCTGCA CAGCGACAGA
    TCGCTCTTTG TGGGCTTAAA TAACGGAGTG CTGAAAATTC CTCTGGAGAG ATGCTCCATG
    TACAGAACAG AAGGGGAATG CCTAGGAGCC AGAGACCCTT ACTGTGGCTG GGATAACAAG
    CAGAAGCGAT GCACCACCAT TGAGGACAGC TCCAACATGA GCCTATGGAC TCAAAATATT
    ACTGAGTGCC CAGTGAAAAA CCTGACAACA AATGGAAGAC TGGGGCCCTG GTCCCCATGG
    CAACCGTGTG AGCATTCAGA TGGGGACAGC ACAGGCTCCT GTATGTGTAG GTCACGTTCG
    TGTGACAGCC CTCGTCCTCG CTGTGGAGGT CGCAGCTGTG AAGGGGCCAG GATCGAGGTC
    GCAAATTGTT CAAGAAATGG CGCTTGGACA CCCTGGTCTT CCTGGGCCCT GTGCAGCACC
    TCCTGTGGGA TAGGCTTTCA GGTCCGCCAG CGGTCCTGCA GCAACCCTGC TCCACGGTAT
    GGGGGCAGGG TCTGTGTTGG ACAGAGCAGG GAAGAGAGAT TCTGTAATGA GAATAGTCCA
    TGTCCATTGC CAATTTTTTG GTCTTCGTGG GGTCCCTGGA ATAAATGTAG TGTGAATTGT
    GGTGGAGGAA TTCACTCAAG GCAAAGGACC TGTGAAAATG GAAATACATG TCCAGGCTGT
    GCTGTGGAAT ATAAGACCTG CAACCCAGAG AGCTGCCCAG AAGTGCGTAG GAACACACCC
    TGGACCCCTT GGATGCCTGT CAACATCACC CAGAATGGTG CCCGCCAGGA GCAGCGCTTC
    CGCTATACGT GTCGTGCCCA GCTGTCTGAT CCCCATGAGC TGCAGTTTGG GAGGAAGAAA
    ACTGAGAGTC GATTCTGTCC CAATGATGGC TCAGCTATGT GTGAAACTGA CTCTCTTGTT
    GACGACCTCC TCAAGACTGG TAAGACCTCT GCACGGATTA TCAATGGGGG CTGGTCCTTT
    TGGGGGGCCT GGTCTTCCTG TTCCAGGGAC TGTGAGTTGG GCTTTAGAAT CCGGAAAAGA
    ACATGCACAA ACCCTGAACC TAAAAATGGT GGCTTGCCTT GCGTGGGGTC AGCGATGGAG
    TACCAAGACT GCAACCCACA TCCTTGCCCA GTAAAAGGCT CATGGTCTTG CTGGACTCCT
    TGGTCTCAGT GCTCAGCAAC CTGTGGAGGA GGGCATTACC AGCGCACACG GACTTGCACC
    AACCCAGCTC CATCCAGTGG AGAGGATATC TGCATCGGGC TGCACACTGA GGAGGCTCTC
    TGCAACACCC ACCCATGTGA AGGTGGCTGG TCAGAGTGGT CAGAGTGGAG CCTGTGCAAT
    GAAGAGGGCA TCCAGTATCG CAGCCGTTAC TGTGAGGTAC ACAGCCCAGA CTCTTCTCAG
    TGCGTTGGAA ACAGCACACA ATACCAAGAT TGCCTGTATA ATGAAATTCC TGTGATCCTG
    CCAGCATCCA GCATTGATGA GAGCACAAAC TGTGGAGGTT TTAGTCTCAT CCATCTGATT
    GCCACTGGGG TCTCCTGCTT CTTTGGCTCT AGCCTCTTAA CGTTTGTGAT TTACGTGTAC
    TGCCAGCGCT GCCAGAGGCA GTCCCAGGAG TCGACCGTTA TCCACCCAAC CACTCCCAAC
    CATCTTCATT ACAAGGGCAA CACAACCCCC AAGAATGAGA AGTACACCCC CATGGAGTTC
    AAGACAGGAG ATGCGCTCAT ACCTTCCCTA AACGTGCTGT GCCTCGATCA CGAGGATGCT
    GTAGCAAACA AAGCCTGTGT TGACCCTCAA ATTCAAGCAG TGAGTTCCCT ACTGTACTGC
    ATGGCAAACA TTAAGGATGT GTGTAGGAGC ATTATAGGCC AGAGACAAGA AGTTGAAGCC
    AGGGACTCGT TGAAAAATGT TGTTAGAAAT GTGCGTCAAC CAAAGTTGTA TACTGTAAAG
    ATGAAGGAGA AAGCTTCATA CAATCATTGC TTCTTCCAAA ATGGGATTTT ATCCTTTTGC
    ATAGGAAAAG CCTGGCATAG CAATGGGTTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa11314
    Clone ID Related Accession (Same CDS sequence) XM_004942927.2 , XM_025152596.1 , XM_015289990.2 , XM_015289989.1 , XM_015289990.1 , XM_004942927.3 , XM_015289989.2
    Accession Version XM_015289989.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3453bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product semaphorin-5B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGTTGTTT CAAGATTAAA GGCCATTTCC CTGTCATTGC CAAGCCTTTT TCTTCTTGCT 
    TTTCATCTCT CTGCATCACA GAATGTACCT GAGTACTCTG AAGCTGAACA TCAGCAGTGT
    GTCAGGAAAG AACACCCCAC AATCGCTTTT GAAGATCTGA AGCCTTGGGT GTCCAATTTC
    ACCTATCCAG GTGTGCATGA TTTTTCTCAG CTCGCCCTGG ATGCCAACAG GAACCAGCTC
    ATTGTGGGAG CAAGGAACTA CCTCTTCAGA CTTAGTCTAC ACAACGTTTC TCTTATTCAG
    GCAACAGCAT GGGGTTCAGA TGAAGACACC AGGAGGTCCT GTCAAAGTAA AGGGAAAACA
    GAGGAGGAGT GCCAGAACTA TGTCCGAGTG CTGATTGTTT ATGGCAAGAA GGTTTTTACC
    TGTGGTACCA ATGCCTTTTC ACCAGTGTGT TCCAGCCGTC AGGTAGGAAA TCTTAGCAAA
    ATCATTGACA GAATTAATGG AGTGGCTCGG TGCCCATATG ACCCCCGGCA TAACTCAACA
    GCTGTGATTA CATCACGAGG AGAACTCTAT GCTGCAACTG TAATTGATTT CTCTGGACGG
    GATCCTGCTA TTTACCGCAG CCTTGGAAAT GTGCCCCCAC TCAGGACAGC ACAGTACAAC
    TCCAAATGGC TCAATGAGCC TAACTTTATC GCTGCCTATG ACATCGGTTT GTTCACCTAC
    TTCTTCTTTC GAGAGAATGC GGTAGAACAC GATTGTGGGA AAACAGTATA TTCTCGTGTG
    GCCAGGGTCT GCAAAAACGA CATTGGAGGG AGATTTTTGC TGGAGGACAC GTGGACAACT
    TTCATGAAAG CCAGGCTGAA CTGCTCCAGA GCTGGAGAAA TCCCTTTCTA TTATAATGAA
    CTGCAAAGCA CCTTCTACCT TCCTGAACAA GACCTGATCT ATGGTGTCTT CACTACTAAT
    GTAAACAGCA TAGCTGCCTC AGCAGTGTGT GCCTTCAATC TCAGTGCCAT TACTCAGGCA
    TTTAATGGTC CTTTCCGTTA CCAAGAGAAC CCAAGATCAG CCTGGCTGCC AACAGCAAAC
    CCCATCCCAA ATTTCCAGTG TGGGACACTG AGCGATGACA GCCCGAATGA GAACCTGACC
    GAGAGAGTCC TACAGGACGC GCAGCGCTTG TTCCTGATGA ACGACGTGGT GCAGCCGGTC
    ACTGTAGATC CGTATGTGAC TCAGGACAGC ATTCGATTCT CTAAACTAGT GGTTGATATT
    GTTCAGGGGA AGGATACTCT CTACCACGTG ATGTACATTG GAACAGAGTA TGGCACCATC
    TTGAAGGCCC TTTCTACCAC TAATAGGAGC CTGAGGAGTT GTTATTTAGA GGAAATGCAG
    ATCCTCCCTG ATGGACAACG GGAAGCAATC AAGAGTCTCC AAATCCTGCA CAGCGACAGA
    TCGCTCTTTG TGGGCTTAAA TAACGGAGTG CTGAAAATTC CTCTGGAGAG ATGCTCCATG
    TACAGAACAG AAGGGGAATG CCTAGGAGCC AGAGACCCTT ACTGTGGCTG GGATAACAAG
    CAGAAGCGAT GCACCACCAT TGAGGACAGC TCCAACATGA GCCTATGGAC TCAAAATATT
    ACTGAGTGCC CAGTGAAAAA CCTGACAACA AATGGAAGAC TGGGGCCCTG GTCCCCATGG
    CAACCGTGTG AGCATTCAGA TGGGGACAGC ACAGGCTCCT GTATGTGTAG GTCACGTTCG
    TGTGACAGCC CTCGTCCTCG CTGTGGAGGT CGCAGCTGTG AAGGGGCCAG GATCGAGGTC
    GCAAATTGTT CAAGAAATGG CGCTTGGACA CCCTGGTCTT CCTGGGCCCT GTGCAGCACC
    TCCTGTGGGA TAGGCTTTCA GGTCCGCCAG CGGTCCTGCA GCAACCCTGC TCCACGGTAT
    GGGGGCAGGG TCTGTGTTGG ACAGAGCAGG GAAGAGAGAT TCTGTAATGA GAATAGTCCA
    TGTCCATTGC CAATTTTTTG GTCTTCGTGG GGTCCCTGGA ATAAATGTAG TGTGAATTGT
    GGTGGAGGAA TTCACTCAAG GCAAAGGACC TGTGAAAATG GAAATACATG TCCAGGCTGT
    GCTGTGGAAT ATAAGACCTG CAACCCAGAG AGCTGCCCAG AAGTGCGTAG GAACACACCC
    TGGACCCCTT GGATGCCTGT CAACATCACC CAGAATGGTG CCCGCCAGGA GCAGCGCTTC
    CGCTATACGT GTCGTGCCCA GCTGTCTGAT CCCCATGAGC TGCAGTTTGG GAGGAAGAAA
    ACTGAGAGTC GATTCTGTCC CAATGATGGC TCAGCTATGT GTGAAACTGA CTCTCTTGTT
    GACGACCTCC TCAAGACTGG TAAGACCTCT GCACGGATTA TCAATGGGGG CTGGTCCTTT
    TGGGGGGCCT GGTCTTCCTG TTCCAGGGAC TGTGAGTTGG GCTTTAGAAT CCGGAAAAGA
    ACATGCACAA ACCCTGAACC TAAAAATGGT GGCTTGCCTT GCGTGGGGTC AGCGATGGAG
    TACCAAGACT GCAACCCACA TCCTTGCCCA GTAAAAGGCT CATGGTCTTG CTGGACTCCT
    TGGTCTCAGT GCTCAGCAAC CTGTGGAGGA GGGCATTACC AGCGCACACG GACTTGCACC
    AACCCAGCTC CATCCAGTGG AGAGGATATC TGCATCGGGC TGCACACTGA GGAGGCTCTC
    TGCAACACCC ACCCATGTGA AGGTGGCTGG TCAGAGTGGT CAGAGTGGAG CCTGTGCAAT
    GAAGAGGGCA TCCAGTATCG CAGCCGTTAC TGTGAGGTAC ACAGCCCAGA CTCTTCTCAG
    TGCGTTGGAA ACAGCACACA ATACCAAGAT TGCCTGTATA ATGAAATTCC TGTGATCCTG
    CCAGCATCCA GCATTGATGA GAGCACAAAC TGTGGAGGTT TTAGTCTCAT CCATCTGATT
    GCCACTGGGG TCTCCTGCTT CTTTGGCTCT AGCCTCTTAA CGTTTGTGAT TTACGTGTAC
    TGCCAGCGCT GCCAGAGGCA GTCCCAGGAG TCGACCGTTA TCCACCCAAC CACTCCCAAC
    CATCTTCATT ACAAGGGCAA CACAACCCCC AAGAATGAGA AGTACACCCC CATGGAGTTC
    AAGACAGGAG ATGCGCTCAT ACCTTCCCTA AACGTGCTGT GCCTCGATCA CGAGGATGCT
    GTAGCAAACA AAGCCTGTGT TGACCCTCAA ATTCAAGCAG TGAGTTCCCT ACTGTACTGC
    ATGGCAAACA TTAAGGATGT GTGTAGGAGC ATTATAGGCC AGAGACAAGA AGTTGAAGCC
    AGGGACTCGT TGAAAAATGT TGTTAGAAAT GTGCGTCAAC CAAAGTTGTA TACTGTAAAG
    ATGAAGGAGA AAGCTTCATA CAATCATTGC TTCTTCCAAA ATGGGATTTT ATCCTTTTGC
    ATAGGAAAAG CCTGGCATAG CAATGGGTTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145475.1
    CDS699..4151
    Translation

    Target ORF information:

    RefSeq Version XM_015289989.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (SEMA5B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289989.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGTTGTTT CAAGATTAAA GGCCATTTCC CTGTCATTGC CAAGCCTTTT TCTTCTTGCT 
    TTTCATCTCT CTGCATCACA GAATGTACCT GAGTACTCTG AAGCTGAACA TCAGCAGTGT
    GTCAGGAAAG AACACCCCAC AATCGCTTTT GAAGATCTGA AGCCTTGGGT GTCCAATTTC
    ACCTATCCAG GTGTGCATGA TTTTTCTCAG CTCGCCCTGG ATGCCAACAG GAACCAGCTC
    ATTGTGGGAG CAAGGAACTA CCTCTTCAGA CTTAGTCTAC ACAACGTTTC TCTTATTCAG
    GCAACAGCAT GGGGTTCAGA TGAAGACACC AGGAGGTCCT GTCAAAGTAA AGGGAAAACA
    GAGGAGGAGT GCCAGAACTA TGTCCGAGTG CTGATTGTTT ATGGCAAGAA GGTTTTTACC
    TGTGGTACCA ATGCCTTTTC ACCAGTGTGT TCCAGCCGTC AGGTAGGAAA TCTTAGCAAA
    ATCATTGACA GAATTAATGG AGTGGCTCGG TGCCCATATG ACCCCCGGCA TAACTCAACA
    GCTGTGATTA CATCACGAGG AGAACTCTAT GCTGCAACTG TAATTGATTT CTCTGGACGG
    GATCCTGCTA TTTACCGCAG CCTTGGAAAT GTGCCCCCAC TCAGGACAGC ACAGTACAAC
    TCCAAATGGC TCAATGAGCC TAACTTTATC GCTGCCTATG ACATCGGTTT GTTCACCTAC
    TTCTTCTTTC GAGAGAATGC GGTAGAACAC GATTGTGGGA AAACAGTATA TTCTCGTGTG
    GCCAGGGTCT GCAAAAACGA CATTGGAGGG AGATTTTTGC TGGAGGACAC GTGGACAACT
    TTCATGAAAG CCAGGCTGAA CTGCTCCAGA GCTGGAGAAA TCCCTTTCTA TTATAATGAA
    CTGCAAAGCA CCTTCTACCT TCCTGAACAA GACCTGATCT ATGGTGTCTT CACTACTAAT
    GTAAACAGCA TAGCTGCCTC AGCAGTGTGT GCCTTCAATC TCAGTGCCAT TACTCAGGCA
    TTTAATGGTC CTTTCCGTTA CCAAGAGAAC CCAAGATCAG CCTGGCTGCC AACAGCAAAC
    CCCATCCCAA ATTTCCAGTG TGGGACACTG AGCGATGACA GCCCGAATGA GAACCTGACC
    GAGAGAGTCC TACAGGACGC GCAGCGCTTG TTCCTGATGA ACGACGTGGT GCAGCCGGTC
    ACTGTAGATC CGTATGTGAC TCAGGACAGC ATTCGATTCT CTAAACTAGT GGTTGATATT
    GTTCAGGGGA AGGATACTCT CTACCACGTG ATGTACATTG GAACAGAGTA TGGCACCATC
    TTGAAGGCCC TTTCTACCAC TAATAGGAGC CTGAGGAGTT GTTATTTAGA GGAAATGCAG
    ATCCTCCCTG ATGGACAACG GGAAGCAATC AAGAGTCTCC AAATCCTGCA CAGCGACAGA
    TCGCTCTTTG TGGGCTTAAA TAACGGAGTG CTGAAAATTC CTCTGGAGAG ATGCTCCATG
    TACAGAACAG AAGGGGAATG CCTAGGAGCC AGAGACCCTT ACTGTGGCTG GGATAACAAG
    CAGAAGCGAT GCACCACCAT TGAGGACAGC TCCAACATGA GCCTATGGAC TCAAAATATT
    ACTGAGTGCC CAGTGAAAAA CCTGACAACA AATGGAAGAC TGGGGCCCTG GTCCCCATGG
    CAACCGTGTG AGCATTCAGA TGGGGACAGC ACAGGCTCCT GTATGTGTAG GTCACGTTCG
    TGTGACAGCC CTCGTCCTCG CTGTGGAGGT CGCAGCTGTG AAGGGGCCAG GATCGAGGTC
    GCAAATTGTT CAAGAAATGG CGCTTGGACA CCCTGGTCTT CCTGGGCCCT GTGCAGCACC
    TCCTGTGGGA TAGGCTTTCA GGTCCGCCAG CGGTCCTGCA GCAACCCTGC TCCACGGTAT
    GGGGGCAGGG TCTGTGTTGG ACAGAGCAGG GAAGAGAGAT TCTGTAATGA GAATAGTCCA
    TGTCCATTGC CAATTTTTTG GTCTTCGTGG GGTCCCTGGA ATAAATGTAG TGTGAATTGT
    GGTGGAGGAA TTCACTCAAG GCAAAGGACC TGTGAAAATG GAAATACATG TCCAGGCTGT
    GCTGTGGAAT ATAAGACCTG CAACCCAGAG AGCTGCCCAG AAGTGCGTAG GAACACACCC
    TGGACCCCTT GGATGCCTGT CAACATCACC CAGAATGGTG CCCGCCAGGA GCAGCGCTTC
    CGCTATACGT GTCGTGCCCA GCTGTCTGAT CCCCATGAGC TGCAGTTTGG GAGGAAGAAA
    ACTGAGAGTC GATTCTGTCC CAATGATGGC TCAGCTATGT GTGAAACTGA CTCTCTTGTT
    GACGACCTCC TCAAGACTGG TAAGACCTCT GCACGGATTA TCAATGGGGG CTGGTCCTTT
    TGGGGGGCCT GGTCTTCCTG TTCCAGGGAC TGTGAGTTGG GCTTTAGAAT CCGGAAAAGA
    ACATGCACAA ACCCTGAACC TAAAAATGGT GGCTTGCCTT GCGTGGGGTC AGCGATGGAG
    TACCAAGACT GCAACCCACA TCCTTGCCCA GTAAAAGGCT CATGGTCTTG CTGGACTCCT
    TGGTCTCAGT GCTCAGCAAC CTGTGGAGGA GGGCATTACC AGCGCACACG GACTTGCACC
    AACCCAGCTC CATCCAGTGG AGAGGATATC TGCATCGGGC TGCACACTGA GGAGGCTCTC
    TGCAACACCC ACCCATGTGA AGGTGGCTGG TCAGAGTGGT CAGAGTGGAG CCTGTGCAAT
    GAAGAGGGCA TCCAGTATCG CAGCCGTTAC TGTGAGGTAC ACAGCCCAGA CTCTTCTCAG
    TGCGTTGGAA ACAGCACACA ATACCAAGAT TGCCTGTATA ATGAAATTCC TGTGATCCTG
    CCAGCATCCA GCATTGATGA GAGCACAAAC TGTGGAGGTT TTAGTCTCAT CCATCTGATT
    GCCACTGGGG TCTCCTGCTT CTTTGGCTCT AGCCTCTTAA CGTTTGTGAT TTACGTGTAC
    TGCCAGCGCT GCCAGAGGCA GTCCCAGGAG TCGACCGTTA TCCACCCAAC CACTCCCAAC
    CATCTTCATT ACAAGGGCAA CACAACCCCC AAGAATGAGA AGTACACCCC CATGGAGTTC
    AAGACAGGAG ATGCGCTCAT ACCTTCCCTA AACGTGCTGT GCCTCGATCA CGAGGATGCT
    GTAGCAAACA AAGCCTGTGT TGACCCTCAA ATTCAAGCAG TGAGTTCCCT ACTGTACTGC
    ATGGCAAACA TTAAGGATGT GTGTAGGAGC ATTATAGGCC AGAGACAAGA AGTTGAAGCC
    AGGGACTCGT TGAAAAATGT TGTTAGAAAT GTGCGTCAAC CAAAGTTGTA TACTGTAAAG
    ATGAAGGAGA AAGCTTCATA CAATCATTGC TTCTTCCAAA ATGGGATTTT ATCCTTTTGC
    ATAGGAAAAG CCTGGCATAG CAATGGGTTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa11314
    Clone ID Related Accession (Same CDS sequence) XM_004942927.2 , XM_025152596.1 , XM_015289990.2 , XM_015289989.1 , XM_015289990.1 , XM_004942927.3 , XM_015289989.2
    Accession Version XM_015289990.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3453bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product semaphorin-5B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGTTGTTT CAAGATTAAA GGCCATTTCC CTGTCATTGC CAAGCCTTTT TCTTCTTGCT 
    TTTCATCTCT CTGCATCACA GAATGTACCT GAGTACTCTG AAGCTGAACA TCAGCAGTGT
    GTCAGGAAAG AACACCCCAC AATCGCTTTT GAAGATCTGA AGCCTTGGGT GTCCAATTTC
    ACCTATCCAG GTGTGCATGA TTTTTCTCAG CTCGCCCTGG ATGCCAACAG GAACCAGCTC
    ATTGTGGGAG CAAGGAACTA CCTCTTCAGA CTTAGTCTAC ACAACGTTTC TCTTATTCAG
    GCAACAGCAT GGGGTTCAGA TGAAGACACC AGGAGGTCCT GTCAAAGTAA AGGGAAAACA
    GAGGAGGAGT GCCAGAACTA TGTCCGAGTG CTGATTGTTT ATGGCAAGAA GGTTTTTACC
    TGTGGTACCA ATGCCTTTTC ACCAGTGTGT TCCAGCCGTC AGGTAGGAAA TCTTAGCAAA
    ATCATTGACA GAATTAATGG AGTGGCTCGG TGCCCATATG ACCCCCGGCA TAACTCAACA
    GCTGTGATTA CATCACGAGG AGAACTCTAT GCTGCAACTG TAATTGATTT CTCTGGACGG
    GATCCTGCTA TTTACCGCAG CCTTGGAAAT GTGCCCCCAC TCAGGACAGC ACAGTACAAC
    TCCAAATGGC TCAATGAGCC TAACTTTATC GCTGCCTATG ACATCGGTTT GTTCACCTAC
    TTCTTCTTTC GAGAGAATGC GGTAGAACAC GATTGTGGGA AAACAGTATA TTCTCGTGTG
    GCCAGGGTCT GCAAAAACGA CATTGGAGGG AGATTTTTGC TGGAGGACAC GTGGACAACT
    TTCATGAAAG CCAGGCTGAA CTGCTCCAGA GCTGGAGAAA TCCCTTTCTA TTATAATGAA
    CTGCAAAGCA CCTTCTACCT TCCTGAACAA GACCTGATCT ATGGTGTCTT CACTACTAAT
    GTAAACAGCA TAGCTGCCTC AGCAGTGTGT GCCTTCAATC TCAGTGCCAT TACTCAGGCA
    TTTAATGGTC CTTTCCGTTA CCAAGAGAAC CCAAGATCAG CCTGGCTGCC AACAGCAAAC
    CCCATCCCAA ATTTCCAGTG TGGGACACTG AGCGATGACA GCCCGAATGA GAACCTGACC
    GAGAGAGTCC TACAGGACGC GCAGCGCTTG TTCCTGATGA ACGACGTGGT GCAGCCGGTC
    ACTGTAGATC CGTATGTGAC TCAGGACAGC ATTCGATTCT CTAAACTAGT GGTTGATATT
    GTTCAGGGGA AGGATACTCT CTACCACGTG ATGTACATTG GAACAGAGTA TGGCACCATC
    TTGAAGGCCC TTTCTACCAC TAATAGGAGC CTGAGGAGTT GTTATTTAGA GGAAATGCAG
    ATCCTCCCTG ATGGACAACG GGAAGCAATC AAGAGTCTCC AAATCCTGCA CAGCGACAGA
    TCGCTCTTTG TGGGCTTAAA TAACGGAGTG CTGAAAATTC CTCTGGAGAG ATGCTCCATG
    TACAGAACAG AAGGGGAATG CCTAGGAGCC AGAGACCCTT ACTGTGGCTG GGATAACAAG
    CAGAAGCGAT GCACCACCAT TGAGGACAGC TCCAACATGA GCCTATGGAC TCAAAATATT
    ACTGAGTGCC CAGTGAAAAA CCTGACAACA AATGGAAGAC TGGGGCCCTG GTCCCCATGG
    CAACCGTGTG AGCATTCAGA TGGGGACAGC ACAGGCTCCT GTATGTGTAG GTCACGTTCG
    TGTGACAGCC CTCGTCCTCG CTGTGGAGGT CGCAGCTGTG AAGGGGCCAG GATCGAGGTC
    GCAAATTGTT CAAGAAATGG CGCTTGGACA CCCTGGTCTT CCTGGGCCCT GTGCAGCACC
    TCCTGTGGGA TAGGCTTTCA GGTCCGCCAG CGGTCCTGCA GCAACCCTGC TCCACGGTAT
    GGGGGCAGGG TCTGTGTTGG ACAGAGCAGG GAAGAGAGAT TCTGTAATGA GAATAGTCCA
    TGTCCATTGC CAATTTTTTG GTCTTCGTGG GGTCCCTGGA ATAAATGTAG TGTGAATTGT
    GGTGGAGGAA TTCACTCAAG GCAAAGGACC TGTGAAAATG GAAATACATG TCCAGGCTGT
    GCTGTGGAAT ATAAGACCTG CAACCCAGAG AGCTGCCCAG AAGTGCGTAG GAACACACCC
    TGGACCCCTT GGATGCCTGT CAACATCACC CAGAATGGTG CCCGCCAGGA GCAGCGCTTC
    CGCTATACGT GTCGTGCCCA GCTGTCTGAT CCCCATGAGC TGCAGTTTGG GAGGAAGAAA
    ACTGAGAGTC GATTCTGTCC CAATGATGGC TCAGCTATGT GTGAAACTGA CTCTCTTGTT
    GACGACCTCC TCAAGACTGG TAAGACCTCT GCACGGATTA TCAATGGGGG CTGGTCCTTT
    TGGGGGGCCT GGTCTTCCTG TTCCAGGGAC TGTGAGTTGG GCTTTAGAAT CCGGAAAAGA
    ACATGCACAA ACCCTGAACC TAAAAATGGT GGCTTGCCTT GCGTGGGGTC AGCGATGGAG
    TACCAAGACT GCAACCCACA TCCTTGCCCA GTAAAAGGCT CATGGTCTTG CTGGACTCCT
    TGGTCTCAGT GCTCAGCAAC CTGTGGAGGA GGGCATTACC AGCGCACACG GACTTGCACC
    AACCCAGCTC CATCCAGTGG AGAGGATATC TGCATCGGGC TGCACACTGA GGAGGCTCTC
    TGCAACACCC ACCCATGTGA AGGTGGCTGG TCAGAGTGGT CAGAGTGGAG CCTGTGCAAT
    GAAGAGGGCA TCCAGTATCG CAGCCGTTAC TGTGAGGTAC ACAGCCCAGA CTCTTCTCAG
    TGCGTTGGAA ACAGCACACA ATACCAAGAT TGCCTGTATA ATGAAATTCC TGTGATCCTG
    CCAGCATCCA GCATTGATGA GAGCACAAAC TGTGGAGGTT TTAGTCTCAT CCATCTGATT
    GCCACTGGGG TCTCCTGCTT CTTTGGCTCT AGCCTCTTAA CGTTTGTGAT TTACGTGTAC
    TGCCAGCGCT GCCAGAGGCA GTCCCAGGAG TCGACCGTTA TCCACCCAAC CACTCCCAAC
    CATCTTCATT ACAAGGGCAA CACAACCCCC AAGAATGAGA AGTACACCCC CATGGAGTTC
    AAGACAGGAG ATGCGCTCAT ACCTTCCCTA AACGTGCTGT GCCTCGATCA CGAGGATGCT
    GTAGCAAACA AAGCCTGTGT TGACCCTCAA ATTCAAGCAG TGAGTTCCCT ACTGTACTGC
    ATGGCAAACA TTAAGGATGT GTGTAGGAGC ATTATAGGCC AGAGACAAGA AGTTGAAGCC
    AGGGACTCGT TGAAAAATGT TGTTAGAAAT GTGCGTCAAC CAAAGTTGTA TACTGTAAAG
    ATGAAGGAGA AAGCTTCATA CAATCATTGC TTCTTCCAAA ATGGGATTTT ATCCTTTTGC
    ATAGGAAAAG CCTGGCATAG CAATGGGTTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145476.1
    CDS392..3844
    Translation

    Target ORF information:

    RefSeq Version XM_015289990.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (SEMA5B), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289990.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGTTGTTT CAAGATTAAA GGCCATTTCC CTGTCATTGC CAAGCCTTTT TCTTCTTGCT 
    TTTCATCTCT CTGCATCACA GAATGTACCT GAGTACTCTG AAGCTGAACA TCAGCAGTGT
    GTCAGGAAAG AACACCCCAC AATCGCTTTT GAAGATCTGA AGCCTTGGGT GTCCAATTTC
    ACCTATCCAG GTGTGCATGA TTTTTCTCAG CTCGCCCTGG ATGCCAACAG GAACCAGCTC
    ATTGTGGGAG CAAGGAACTA CCTCTTCAGA CTTAGTCTAC ACAACGTTTC TCTTATTCAG
    GCAACAGCAT GGGGTTCAGA TGAAGACACC AGGAGGTCCT GTCAAAGTAA AGGGAAAACA
    GAGGAGGAGT GCCAGAACTA TGTCCGAGTG CTGATTGTTT ATGGCAAGAA GGTTTTTACC
    TGTGGTACCA ATGCCTTTTC ACCAGTGTGT TCCAGCCGTC AGGTAGGAAA TCTTAGCAAA
    ATCATTGACA GAATTAATGG AGTGGCTCGG TGCCCATATG ACCCCCGGCA TAACTCAACA
    GCTGTGATTA CATCACGAGG AGAACTCTAT GCTGCAACTG TAATTGATTT CTCTGGACGG
    GATCCTGCTA TTTACCGCAG CCTTGGAAAT GTGCCCCCAC TCAGGACAGC ACAGTACAAC
    TCCAAATGGC TCAATGAGCC TAACTTTATC GCTGCCTATG ACATCGGTTT GTTCACCTAC
    TTCTTCTTTC GAGAGAATGC GGTAGAACAC GATTGTGGGA AAACAGTATA TTCTCGTGTG
    GCCAGGGTCT GCAAAAACGA CATTGGAGGG AGATTTTTGC TGGAGGACAC GTGGACAACT
    TTCATGAAAG CCAGGCTGAA CTGCTCCAGA GCTGGAGAAA TCCCTTTCTA TTATAATGAA
    CTGCAAAGCA CCTTCTACCT TCCTGAACAA GACCTGATCT ATGGTGTCTT CACTACTAAT
    GTAAACAGCA TAGCTGCCTC AGCAGTGTGT GCCTTCAATC TCAGTGCCAT TACTCAGGCA
    TTTAATGGTC CTTTCCGTTA CCAAGAGAAC CCAAGATCAG CCTGGCTGCC AACAGCAAAC
    CCCATCCCAA ATTTCCAGTG TGGGACACTG AGCGATGACA GCCCGAATGA GAACCTGACC
    GAGAGAGTCC TACAGGACGC GCAGCGCTTG TTCCTGATGA ACGACGTGGT GCAGCCGGTC
    ACTGTAGATC CGTATGTGAC TCAGGACAGC ATTCGATTCT CTAAACTAGT GGTTGATATT
    GTTCAGGGGA AGGATACTCT CTACCACGTG ATGTACATTG GAACAGAGTA TGGCACCATC
    TTGAAGGCCC TTTCTACCAC TAATAGGAGC CTGAGGAGTT GTTATTTAGA GGAAATGCAG
    ATCCTCCCTG ATGGACAACG GGAAGCAATC AAGAGTCTCC AAATCCTGCA CAGCGACAGA
    TCGCTCTTTG TGGGCTTAAA TAACGGAGTG CTGAAAATTC CTCTGGAGAG ATGCTCCATG
    TACAGAACAG AAGGGGAATG CCTAGGAGCC AGAGACCCTT ACTGTGGCTG GGATAACAAG
    CAGAAGCGAT GCACCACCAT TGAGGACAGC TCCAACATGA GCCTATGGAC TCAAAATATT
    ACTGAGTGCC CAGTGAAAAA CCTGACAACA AATGGAAGAC TGGGGCCCTG GTCCCCATGG
    CAACCGTGTG AGCATTCAGA TGGGGACAGC ACAGGCTCCT GTATGTGTAG GTCACGTTCG
    TGTGACAGCC CTCGTCCTCG CTGTGGAGGT CGCAGCTGTG AAGGGGCCAG GATCGAGGTC
    GCAAATTGTT CAAGAAATGG CGCTTGGACA CCCTGGTCTT CCTGGGCCCT GTGCAGCACC
    TCCTGTGGGA TAGGCTTTCA GGTCCGCCAG CGGTCCTGCA GCAACCCTGC TCCACGGTAT
    GGGGGCAGGG TCTGTGTTGG ACAGAGCAGG GAAGAGAGAT TCTGTAATGA GAATAGTCCA
    TGTCCATTGC CAATTTTTTG GTCTTCGTGG GGTCCCTGGA ATAAATGTAG TGTGAATTGT
    GGTGGAGGAA TTCACTCAAG GCAAAGGACC TGTGAAAATG GAAATACATG TCCAGGCTGT
    GCTGTGGAAT ATAAGACCTG CAACCCAGAG AGCTGCCCAG AAGTGCGTAG GAACACACCC
    TGGACCCCTT GGATGCCTGT CAACATCACC CAGAATGGTG CCCGCCAGGA GCAGCGCTTC
    CGCTATACGT GTCGTGCCCA GCTGTCTGAT CCCCATGAGC TGCAGTTTGG GAGGAAGAAA
    ACTGAGAGTC GATTCTGTCC CAATGATGGC TCAGCTATGT GTGAAACTGA CTCTCTTGTT
    GACGACCTCC TCAAGACTGG TAAGACCTCT GCACGGATTA TCAATGGGGG CTGGTCCTTT
    TGGGGGGCCT GGTCTTCCTG TTCCAGGGAC TGTGAGTTGG GCTTTAGAAT CCGGAAAAGA
    ACATGCACAA ACCCTGAACC TAAAAATGGT GGCTTGCCTT GCGTGGGGTC AGCGATGGAG
    TACCAAGACT GCAACCCACA TCCTTGCCCA GTAAAAGGCT CATGGTCTTG CTGGACTCCT
    TGGTCTCAGT GCTCAGCAAC CTGTGGAGGA GGGCATTACC AGCGCACACG GACTTGCACC
    AACCCAGCTC CATCCAGTGG AGAGGATATC TGCATCGGGC TGCACACTGA GGAGGCTCTC
    TGCAACACCC ACCCATGTGA AGGTGGCTGG TCAGAGTGGT CAGAGTGGAG CCTGTGCAAT
    GAAGAGGGCA TCCAGTATCG CAGCCGTTAC TGTGAGGTAC ACAGCCCAGA CTCTTCTCAG
    TGCGTTGGAA ACAGCACACA ATACCAAGAT TGCCTGTATA ATGAAATTCC TGTGATCCTG
    CCAGCATCCA GCATTGATGA GAGCACAAAC TGTGGAGGTT TTAGTCTCAT CCATCTGATT
    GCCACTGGGG TCTCCTGCTT CTTTGGCTCT AGCCTCTTAA CGTTTGTGAT TTACGTGTAC
    TGCCAGCGCT GCCAGAGGCA GTCCCAGGAG TCGACCGTTA TCCACCCAAC CACTCCCAAC
    CATCTTCATT ACAAGGGCAA CACAACCCCC AAGAATGAGA AGTACACCCC CATGGAGTTC
    AAGACAGGAG ATGCGCTCAT ACCTTCCCTA AACGTGCTGT GCCTCGATCA CGAGGATGCT
    GTAGCAAACA AAGCCTGTGT TGACCCTCAA ATTCAAGCAG TGAGTTCCCT ACTGTACTGC
    ATGGCAAACA TTAAGGATGT GTGTAGGAGC ATTATAGGCC AGAGACAAGA AGTTGAAGCC
    AGGGACTCGT TGAAAAATGT TGTTAGAAAT GTGCGTCAAC CAAAGTTGTA TACTGTAAAG
    ATGAAGGAGA AAGCTTCATA CAATCATTGC TTCTTCCAAA ATGGGATTTT ATCCTTTTGC
    ATAGGAAAAG CCTGGCATAG CAATGGGTTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa11314
    Clone ID Related Accession (Same CDS sequence) XM_004942927.2 , XM_025152596.1 , XM_015289990.2 , XM_015289989.1 , XM_015289990.1 , XM_004942927.3 , XM_015289989.2
    Accession Version XM_004942927.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3453bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product semaphorin-5B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004942927.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGTTGTTT CAAGATTAAA GGCCATTTCC CTGTCATTGC CAAGCCTTTT TCTTCTTGCT 
    TTTCATCTCT CTGCATCACA GAATGTACCT GAGTACTCTG AAGCTGAACA TCAGCAGTGT
    GTCAGGAAAG AACACCCCAC AATCGCTTTT GAAGATCTGA AGCCTTGGGT GTCCAATTTC
    ACCTATCCAG GTGTGCATGA TTTTTCTCAG CTCGCCCTGG ATGCCAACAG GAACCAGCTC
    ATTGTGGGAG CAAGGAACTA CCTCTTCAGA CTTAGTCTAC ACAACGTTTC TCTTATTCAG
    GCAACAGCAT GGGGTTCAGA TGAAGACACC AGGAGGTCCT GTCAAAGTAA AGGGAAAACA
    GAGGAGGAGT GCCAGAACTA TGTCCGAGTG CTGATTGTTT ATGGCAAGAA GGTTTTTACC
    TGTGGTACCA ATGCCTTTTC ACCAGTGTGT TCCAGCCGTC AGGTAGGAAA TCTTAGCAAA
    ATCATTGACA GAATTAATGG AGTGGCTCGG TGCCCATATG ACCCCCGGCA TAACTCAACA
    GCTGTGATTA CATCACGAGG AGAACTCTAT GCTGCAACTG TAATTGATTT CTCTGGACGG
    GATCCTGCTA TTTACCGCAG CCTTGGAAAT GTGCCCCCAC TCAGGACAGC ACAGTACAAC
    TCCAAATGGC TCAATGAGCC TAACTTTATC GCTGCCTATG ACATCGGTTT GTTCACCTAC
    TTCTTCTTTC GAGAGAATGC GGTAGAACAC GATTGTGGGA AAACAGTATA TTCTCGTGTG
    GCCAGGGTCT GCAAAAACGA CATTGGAGGG AGATTTTTGC TGGAGGACAC GTGGACAACT
    TTCATGAAAG CCAGGCTGAA CTGCTCCAGA GCTGGAGAAA TCCCTTTCTA TTATAATGAA
    CTGCAAAGCA CCTTCTACCT TCCTGAACAA GACCTGATCT ATGGTGTCTT CACTACTAAT
    GTAAACAGCA TAGCTGCCTC AGCAGTGTGT GCCTTCAATC TCAGTGCCAT TACTCAGGCA
    TTTAATGGTC CTTTCCGTTA CCAAGAGAAC CCAAGATCAG CCTGGCTGCC AACAGCAAAC
    CCCATCCCAA ATTTCCAGTG TGGGACACTG AGCGATGACA GCCCGAATGA GAACCTGACC
    GAGAGAGTCC TACAGGACGC GCAGCGCTTG TTCCTGATGA ACGACGTGGT GCAGCCGGTC
    ACTGTAGATC CGTATGTGAC TCAGGACAGC ATTCGATTCT CTAAACTAGT GGTTGATATT
    GTTCAGGGGA AGGATACTCT CTACCACGTG ATGTACATTG GAACAGAGTA TGGCACCATC
    TTGAAGGCCC TTTCTACCAC TAATAGGAGC CTGAGGAGTT GTTATTTAGA GGAAATGCAG
    ATCCTCCCTG ATGGACAACG GGAAGCAATC AAGAGTCTCC AAATCCTGCA CAGCGACAGA
    TCGCTCTTTG TGGGCTTAAA TAACGGAGTG CTGAAAATTC CTCTGGAGAG ATGCTCCATG
    TACAGAACAG AAGGGGAATG CCTAGGAGCC AGAGACCCTT ACTGTGGCTG GGATAACAAG
    CAGAAGCGAT GCACCACCAT TGAGGACAGC TCCAACATGA GCCTATGGAC TCAAAATATT
    ACTGAGTGCC CAGTGAAAAA CCTGACAACA AATGGAAGAC TGGGGCCCTG GTCCCCATGG
    CAACCGTGTG AGCATTCAGA TGGGGACAGC ACAGGCTCCT GTATGTGTAG GTCACGTTCG
    TGTGACAGCC CTCGTCCTCG CTGTGGAGGT CGCAGCTGTG AAGGGGCCAG GATCGAGGTC
    GCAAATTGTT CAAGAAATGG CGCTTGGACA CCCTGGTCTT CCTGGGCCCT GTGCAGCACC
    TCCTGTGGGA TAGGCTTTCA GGTCCGCCAG CGGTCCTGCA GCAACCCTGC TCCACGGTAT
    GGGGGCAGGG TCTGTGTTGG ACAGAGCAGG GAAGAGAGAT TCTGTAATGA GAATAGTCCA
    TGTCCATTGC CAATTTTTTG GTCTTCGTGG GGTCCCTGGA ATAAATGTAG TGTGAATTGT
    GGTGGAGGAA TTCACTCAAG GCAAAGGACC TGTGAAAATG GAAATACATG TCCAGGCTGT
    GCTGTGGAAT ATAAGACCTG CAACCCAGAG AGCTGCCCAG AAGTGCGTAG GAACACACCC
    TGGACCCCTT GGATGCCTGT CAACATCACC CAGAATGGTG CCCGCCAGGA GCAGCGCTTC
    CGCTATACGT GTCGTGCCCA GCTGTCTGAT CCCCATGAGC TGCAGTTTGG GAGGAAGAAA
    ACTGAGAGTC GATTCTGTCC CAATGATGGC TCAGCTATGT GTGAAACTGA CTCTCTTGTT
    GACGACCTCC TCAAGACTGG TAAGACCTCT GCACGGATTA TCAATGGGGG CTGGTCCTTT
    TGGGGGGCCT GGTCTTCCTG TTCCAGGGAC TGTGAGTTGG GCTTTAGAAT CCGGAAAAGA
    ACATGCACAA ACCCTGAACC TAAAAATGGT GGCTTGCCTT GCGTGGGGTC AGCGATGGAG
    TACCAAGACT GCAACCCACA TCCTTGCCCA GTAAAAGGCT CATGGTCTTG CTGGACTCCT
    TGGTCTCAGT GCTCAGCAAC CTGTGGAGGA GGGCATTACC AGCGCACACG GACTTGCACC
    AACCCAGCTC CATCCAGTGG AGAGGATATC TGCATCGGGC TGCACACTGA GGAGGCTCTC
    TGCAACACCC ACCCATGTGA AGGTGGCTGG TCAGAGTGGT CAGAGTGGAG CCTGTGCAAT
    GAAGAGGGCA TCCAGTATCG CAGCCGTTAC TGTGAGGTAC ACAGCCCAGA CTCTTCTCAG
    TGCGTTGGAA ACAGCACACA ATACCAAGAT TGCCTGTATA ATGAAATTCC TGTGATCCTG
    CCAGCATCCA GCATTGATGA GAGCACAAAC TGTGGAGGTT TTAGTCTCAT CCATCTGATT
    GCCACTGGGG TCTCCTGCTT CTTTGGCTCT AGCCTCTTAA CGTTTGTGAT TTACGTGTAC
    TGCCAGCGCT GCCAGAGGCA GTCCCAGGAG TCGACCGTTA TCCACCCAAC CACTCCCAAC
    CATCTTCATT ACAAGGGCAA CACAACCCCC AAGAATGAGA AGTACACCCC CATGGAGTTC
    AAGACAGGAG ATGCGCTCAT ACCTTCCCTA AACGTGCTGT GCCTCGATCA CGAGGATGCT
    GTAGCAAACA AAGCCTGTGT TGACCCTCAA ATTCAAGCAG TGAGTTCCCT ACTGTACTGC
    ATGGCAAACA TTAAGGATGT GTGTAGGAGC ATTATAGGCC AGAGACAAGA AGTTGAAGCC
    AGGGACTCGT TGAAAAATGT TGTTAGAAAT GTGCGTCAAC CAAAGTTGTA TACTGTAAAG
    ATGAAGGAGA AAGCTTCATA CAATCATTGC TTCTTCCAAA ATGGGATTTT ATCCTTTTGC
    ATAGGAAAAG CCTGGCATAG CAATGGGTTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004942984.1
    CDS598..4050
    Translation

    Target ORF information:

    RefSeq Version XM_004942927.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus semaphorin 5B (SEMA5B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004942927.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGTTGTTT CAAGATTAAA GGCCATTTCC CTGTCATTGC CAAGCCTTTT TCTTCTTGCT 
    TTTCATCTCT CTGCATCACA GAATGTACCT GAGTACTCTG AAGCTGAACA TCAGCAGTGT
    GTCAGGAAAG AACACCCCAC AATCGCTTTT GAAGATCTGA AGCCTTGGGT GTCCAATTTC
    ACCTATCCAG GTGTGCATGA TTTTTCTCAG CTCGCCCTGG ATGCCAACAG GAACCAGCTC
    ATTGTGGGAG CAAGGAACTA CCTCTTCAGA CTTAGTCTAC ACAACGTTTC TCTTATTCAG
    GCAACAGCAT GGGGTTCAGA TGAAGACACC AGGAGGTCCT GTCAAAGTAA AGGGAAAACA
    GAGGAGGAGT GCCAGAACTA TGTCCGAGTG CTGATTGTTT ATGGCAAGAA GGTTTTTACC
    TGTGGTACCA ATGCCTTTTC ACCAGTGTGT TCCAGCCGTC AGGTAGGAAA TCTTAGCAAA
    ATCATTGACA GAATTAATGG AGTGGCTCGG TGCCCATATG ACCCCCGGCA TAACTCAACA
    GCTGTGATTA CATCACGAGG AGAACTCTAT GCTGCAACTG TAATTGATTT CTCTGGACGG
    GATCCTGCTA TTTACCGCAG CCTTGGAAAT GTGCCCCCAC TCAGGACAGC ACAGTACAAC
    TCCAAATGGC TCAATGAGCC TAACTTTATC GCTGCCTATG ACATCGGTTT GTTCACCTAC
    TTCTTCTTTC GAGAGAATGC GGTAGAACAC GATTGTGGGA AAACAGTATA TTCTCGTGTG
    GCCAGGGTCT GCAAAAACGA CATTGGAGGG AGATTTTTGC TGGAGGACAC GTGGACAACT
    TTCATGAAAG CCAGGCTGAA CTGCTCCAGA GCTGGAGAAA TCCCTTTCTA TTATAATGAA
    CTGCAAAGCA CCTTCTACCT TCCTGAACAA GACCTGATCT ATGGTGTCTT CACTACTAAT
    GTAAACAGCA TAGCTGCCTC AGCAGTGTGT GCCTTCAATC TCAGTGCCAT TACTCAGGCA
    TTTAATGGTC CTTTCCGTTA CCAAGAGAAC CCAAGATCAG CCTGGCTGCC AACAGCAAAC
    CCCATCCCAA ATTTCCAGTG TGGGACACTG AGCGATGACA GCCCGAATGA GAACCTGACC
    GAGAGAGTCC TACAGGACGC GCAGCGCTTG TTCCTGATGA ACGACGTGGT GCAGCCGGTC
    ACTGTAGATC CGTATGTGAC TCAGGACAGC ATTCGATTCT CTAAACTAGT GGTTGATATT
    GTTCAGGGGA AGGATACTCT CTACCACGTG ATGTACATTG GAACAGAGTA TGGCACCATC
    TTGAAGGCCC TTTCTACCAC TAATAGGAGC CTGAGGAGTT GTTATTTAGA GGAAATGCAG
    ATCCTCCCTG ATGGACAACG GGAAGCAATC AAGAGTCTCC AAATCCTGCA CAGCGACAGA
    TCGCTCTTTG TGGGCTTAAA TAACGGAGTG CTGAAAATTC CTCTGGAGAG ATGCTCCATG
    TACAGAACAG AAGGGGAATG CCTAGGAGCC AGAGACCCTT ACTGTGGCTG GGATAACAAG
    CAGAAGCGAT GCACCACCAT TGAGGACAGC TCCAACATGA GCCTATGGAC TCAAAATATT
    ACTGAGTGCC CAGTGAAAAA CCTGACAACA AATGGAAGAC TGGGGCCCTG GTCCCCATGG
    CAACCGTGTG AGCATTCAGA TGGGGACAGC ACAGGCTCCT GTATGTGTAG GTCACGTTCG
    TGTGACAGCC CTCGTCCTCG CTGTGGAGGT CGCAGCTGTG AAGGGGCCAG GATCGAGGTC
    GCAAATTGTT CAAGAAATGG CGCTTGGACA CCCTGGTCTT CCTGGGCCCT GTGCAGCACC
    TCCTGTGGGA TAGGCTTTCA GGTCCGCCAG CGGTCCTGCA GCAACCCTGC TCCACGGTAT
    GGGGGCAGGG TCTGTGTTGG ACAGAGCAGG GAAGAGAGAT TCTGTAATGA GAATAGTCCA
    TGTCCATTGC CAATTTTTTG GTCTTCGTGG GGTCCCTGGA ATAAATGTAG TGTGAATTGT
    GGTGGAGGAA TTCACTCAAG GCAAAGGACC TGTGAAAATG GAAATACATG TCCAGGCTGT
    GCTGTGGAAT ATAAGACCTG CAACCCAGAG AGCTGCCCAG AAGTGCGTAG GAACACACCC
    TGGACCCCTT GGATGCCTGT CAACATCACC CAGAATGGTG CCCGCCAGGA GCAGCGCTTC
    CGCTATACGT GTCGTGCCCA GCTGTCTGAT CCCCATGAGC TGCAGTTTGG GAGGAAGAAA
    ACTGAGAGTC GATTCTGTCC CAATGATGGC TCAGCTATGT GTGAAACTGA CTCTCTTGTT
    GACGACCTCC TCAAGACTGG TAAGACCTCT GCACGGATTA TCAATGGGGG CTGGTCCTTT
    TGGGGGGCCT GGTCTTCCTG TTCCAGGGAC TGTGAGTTGG GCTTTAGAAT CCGGAAAAGA
    ACATGCACAA ACCCTGAACC TAAAAATGGT GGCTTGCCTT GCGTGGGGTC AGCGATGGAG
    TACCAAGACT GCAACCCACA TCCTTGCCCA GTAAAAGGCT CATGGTCTTG CTGGACTCCT
    TGGTCTCAGT GCTCAGCAAC CTGTGGAGGA GGGCATTACC AGCGCACACG GACTTGCACC
    AACCCAGCTC CATCCAGTGG AGAGGATATC TGCATCGGGC TGCACACTGA GGAGGCTCTC
    TGCAACACCC ACCCATGTGA AGGTGGCTGG TCAGAGTGGT CAGAGTGGAG CCTGTGCAAT
    GAAGAGGGCA TCCAGTATCG CAGCCGTTAC TGTGAGGTAC ACAGCCCAGA CTCTTCTCAG
    TGCGTTGGAA ACAGCACACA ATACCAAGAT TGCCTGTATA ATGAAATTCC TGTGATCCTG
    CCAGCATCCA GCATTGATGA GAGCACAAAC TGTGGAGGTT TTAGTCTCAT CCATCTGATT
    GCCACTGGGG TCTCCTGCTT CTTTGGCTCT AGCCTCTTAA CGTTTGTGAT TTACGTGTAC
    TGCCAGCGCT GCCAGAGGCA GTCCCAGGAG TCGACCGTTA TCCACCCAAC CACTCCCAAC
    CATCTTCATT ACAAGGGCAA CACAACCCCC AAGAATGAGA AGTACACCCC CATGGAGTTC
    AAGACAGGAG ATGCGCTCAT ACCTTCCCTA AACGTGCTGT GCCTCGATCA CGAGGATGCT
    GTAGCAAACA AAGCCTGTGT TGACCCTCAA ATTCAAGCAG TGAGTTCCCT ACTGTACTGC
    ATGGCAAACA TTAAGGATGT GTGTAGGAGC ATTATAGGCC AGAGACAAGA AGTTGAAGCC
    AGGGACTCGT TGAAAAATGT TGTTAGAAAT GTGCGTCAAC CAAAGTTGTA TACTGTAAAG
    ATGAAGGAGA AAGCTTCATA CAATCATTGC TTCTTCCAAA ATGGGATTTT ATCCTTTTGC
    ATAGGAAAAG CCTGGCATAG CAATGGGTTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa11314
    Clone ID Related Accession (Same CDS sequence) XM_004942927.2 , XM_025152596.1 , XM_015289990.2 , XM_015289989.1 , XM_015289990.1 , XM_004942927.3 , XM_015289989.2
    Accession Version XM_015289989.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3453bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product semaphorin-5B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289989.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGTTGTTT CAAGATTAAA GGCCATTTCC CTGTCATTGC CAAGCCTTTT TCTTCTTGCT 
    TTTCATCTCT CTGCATCACA GAATGTACCT GAGTACTCTG AAGCTGAACA TCAGCAGTGT
    GTCAGGAAAG AACACCCCAC AATCGCTTTT GAAGATCTGA AGCCTTGGGT GTCCAATTTC
    ACCTATCCAG GTGTGCATGA TTTTTCTCAG CTCGCCCTGG ATGCCAACAG GAACCAGCTC
    ATTGTGGGAG CAAGGAACTA CCTCTTCAGA CTTAGTCTAC ACAACGTTTC TCTTATTCAG
    GCAACAGCAT GGGGTTCAGA TGAAGACACC AGGAGGTCCT GTCAAAGTAA AGGGAAAACA
    GAGGAGGAGT GCCAGAACTA TGTCCGAGTG CTGATTGTTT ATGGCAAGAA GGTTTTTACC
    TGTGGTACCA ATGCCTTTTC ACCAGTGTGT TCCAGCCGTC AGGTAGGAAA TCTTAGCAAA
    ATCATTGACA GAATTAATGG AGTGGCTCGG TGCCCATATG ACCCCCGGCA TAACTCAACA
    GCTGTGATTA CATCACGAGG AGAACTCTAT GCTGCAACTG TAATTGATTT CTCTGGACGG
    GATCCTGCTA TTTACCGCAG CCTTGGAAAT GTGCCCCCAC TCAGGACAGC ACAGTACAAC
    TCCAAATGGC TCAATGAGCC TAACTTTATC GCTGCCTATG ACATCGGTTT GTTCACCTAC
    TTCTTCTTTC GAGAGAATGC GGTAGAACAC GATTGTGGGA AAACAGTATA TTCTCGTGTG
    GCCAGGGTCT GCAAAAACGA CATTGGAGGG AGATTTTTGC TGGAGGACAC GTGGACAACT
    TTCATGAAAG CCAGGCTGAA CTGCTCCAGA GCTGGAGAAA TCCCTTTCTA TTATAATGAA
    CTGCAAAGCA CCTTCTACCT TCCTGAACAA GACCTGATCT ATGGTGTCTT CACTACTAAT
    GTAAACAGCA TAGCTGCCTC AGCAGTGTGT GCCTTCAATC TCAGTGCCAT TACTCAGGCA
    TTTAATGGTC CTTTCCGTTA CCAAGAGAAC CCAAGATCAG CCTGGCTGCC AACAGCAAAC
    CCCATCCCAA ATTTCCAGTG TGGGACACTG AGCGATGACA GCCCGAATGA GAACCTGACC
    GAGAGAGTCC TACAGGACGC GCAGCGCTTG TTCCTGATGA ACGACGTGGT GCAGCCGGTC
    ACTGTAGATC CGTATGTGAC TCAGGACAGC ATTCGATTCT CTAAACTAGT GGTTGATATT
    GTTCAGGGGA AGGATACTCT CTACCACGTG ATGTACATTG GAACAGAGTA TGGCACCATC
    TTGAAGGCCC TTTCTACCAC TAATAGGAGC CTGAGGAGTT GTTATTTAGA GGAAATGCAG
    ATCCTCCCTG ATGGACAACG GGAAGCAATC AAGAGTCTCC AAATCCTGCA CAGCGACAGA
    TCGCTCTTTG TGGGCTTAAA TAACGGAGTG CTGAAAATTC CTCTGGAGAG ATGCTCCATG
    TACAGAACAG AAGGGGAATG CCTAGGAGCC AGAGACCCTT ACTGTGGCTG GGATAACAAG
    CAGAAGCGAT GCACCACCAT TGAGGACAGC TCCAACATGA GCCTATGGAC TCAAAATATT
    ACTGAGTGCC CAGTGAAAAA CCTGACAACA AATGGAAGAC TGGGGCCCTG GTCCCCATGG
    CAACCGTGTG AGCATTCAGA TGGGGACAGC ACAGGCTCCT GTATGTGTAG GTCACGTTCG
    TGTGACAGCC CTCGTCCTCG CTGTGGAGGT CGCAGCTGTG AAGGGGCCAG GATCGAGGTC
    GCAAATTGTT CAAGAAATGG CGCTTGGACA CCCTGGTCTT CCTGGGCCCT GTGCAGCACC
    TCCTGTGGGA TAGGCTTTCA GGTCCGCCAG CGGTCCTGCA GCAACCCTGC TCCACGGTAT
    GGGGGCAGGG TCTGTGTTGG ACAGAGCAGG GAAGAGAGAT TCTGTAATGA GAATAGTCCA
    TGTCCATTGC CAATTTTTTG GTCTTCGTGG GGTCCCTGGA ATAAATGTAG TGTGAATTGT
    GGTGGAGGAA TTCACTCAAG GCAAAGGACC TGTGAAAATG GAAATACATG TCCAGGCTGT
    GCTGTGGAAT ATAAGACCTG CAACCCAGAG AGCTGCCCAG AAGTGCGTAG GAACACACCC
    TGGACCCCTT GGATGCCTGT CAACATCACC CAGAATGGTG CCCGCCAGGA GCAGCGCTTC
    CGCTATACGT GTCGTGCCCA GCTGTCTGAT CCCCATGAGC TGCAGTTTGG GAGGAAGAAA
    ACTGAGAGTC GATTCTGTCC CAATGATGGC TCAGCTATGT GTGAAACTGA CTCTCTTGTT
    GACGACCTCC TCAAGACTGG TAAGACCTCT GCACGGATTA TCAATGGGGG CTGGTCCTTT
    TGGGGGGCCT GGTCTTCCTG TTCCAGGGAC TGTGAGTTGG GCTTTAGAAT CCGGAAAAGA
    ACATGCACAA ACCCTGAACC TAAAAATGGT GGCTTGCCTT GCGTGGGGTC AGCGATGGAG
    TACCAAGACT GCAACCCACA TCCTTGCCCA GTAAAAGGCT CATGGTCTTG CTGGACTCCT
    TGGTCTCAGT GCTCAGCAAC CTGTGGAGGA GGGCATTACC AGCGCACACG GACTTGCACC
    AACCCAGCTC CATCCAGTGG AGAGGATATC TGCATCGGGC TGCACACTGA GGAGGCTCTC
    TGCAACACCC ACCCATGTGA AGGTGGCTGG TCAGAGTGGT CAGAGTGGAG CCTGTGCAAT
    GAAGAGGGCA TCCAGTATCG CAGCCGTTAC TGTGAGGTAC ACAGCCCAGA CTCTTCTCAG
    TGCGTTGGAA ACAGCACACA ATACCAAGAT TGCCTGTATA ATGAAATTCC TGTGATCCTG
    CCAGCATCCA GCATTGATGA GAGCACAAAC TGTGGAGGTT TTAGTCTCAT CCATCTGATT
    GCCACTGGGG TCTCCTGCTT CTTTGGCTCT AGCCTCTTAA CGTTTGTGAT TTACGTGTAC
    TGCCAGCGCT GCCAGAGGCA GTCCCAGGAG TCGACCGTTA TCCACCCAAC CACTCCCAAC
    CATCTTCATT ACAAGGGCAA CACAACCCCC AAGAATGAGA AGTACACCCC CATGGAGTTC
    AAGACAGGAG ATGCGCTCAT ACCTTCCCTA AACGTGCTGT GCCTCGATCA CGAGGATGCT
    GTAGCAAACA AAGCCTGTGT TGACCCTCAA ATTCAAGCAG TGAGTTCCCT ACTGTACTGC
    ATGGCAAACA TTAAGGATGT GTGTAGGAGC ATTATAGGCC AGAGACAAGA AGTTGAAGCC
    AGGGACTCGT TGAAAAATGT TGTTAGAAAT GTGCGTCAAC CAAAGTTGTA TACTGTAAAG
    ATGAAGGAGA AAGCTTCATA CAATCATTGC TTCTTCCAAA ATGGGATTTT ATCCTTTTGC
    ATAGGAAAAG CCTGGCATAG CAATGGGTTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145475.1
    CDS689..4141
    Translation

    Target ORF information:

    RefSeq Version XM_015289989.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus semaphorin 5B (SEMA5B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289989.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGTTGTTT CAAGATTAAA GGCCATTTCC CTGTCATTGC CAAGCCTTTT TCTTCTTGCT 
    TTTCATCTCT CTGCATCACA GAATGTACCT GAGTACTCTG AAGCTGAACA TCAGCAGTGT
    GTCAGGAAAG AACACCCCAC AATCGCTTTT GAAGATCTGA AGCCTTGGGT GTCCAATTTC
    ACCTATCCAG GTGTGCATGA TTTTTCTCAG CTCGCCCTGG ATGCCAACAG GAACCAGCTC
    ATTGTGGGAG CAAGGAACTA CCTCTTCAGA CTTAGTCTAC ACAACGTTTC TCTTATTCAG
    GCAACAGCAT GGGGTTCAGA TGAAGACACC AGGAGGTCCT GTCAAAGTAA AGGGAAAACA
    GAGGAGGAGT GCCAGAACTA TGTCCGAGTG CTGATTGTTT ATGGCAAGAA GGTTTTTACC
    TGTGGTACCA ATGCCTTTTC ACCAGTGTGT TCCAGCCGTC AGGTAGGAAA TCTTAGCAAA
    ATCATTGACA GAATTAATGG AGTGGCTCGG TGCCCATATG ACCCCCGGCA TAACTCAACA
    GCTGTGATTA CATCACGAGG AGAACTCTAT GCTGCAACTG TAATTGATTT CTCTGGACGG
    GATCCTGCTA TTTACCGCAG CCTTGGAAAT GTGCCCCCAC TCAGGACAGC ACAGTACAAC
    TCCAAATGGC TCAATGAGCC TAACTTTATC GCTGCCTATG ACATCGGTTT GTTCACCTAC
    TTCTTCTTTC GAGAGAATGC GGTAGAACAC GATTGTGGGA AAACAGTATA TTCTCGTGTG
    GCCAGGGTCT GCAAAAACGA CATTGGAGGG AGATTTTTGC TGGAGGACAC GTGGACAACT
    TTCATGAAAG CCAGGCTGAA CTGCTCCAGA GCTGGAGAAA TCCCTTTCTA TTATAATGAA
    CTGCAAAGCA CCTTCTACCT TCCTGAACAA GACCTGATCT ATGGTGTCTT CACTACTAAT
    GTAAACAGCA TAGCTGCCTC AGCAGTGTGT GCCTTCAATC TCAGTGCCAT TACTCAGGCA
    TTTAATGGTC CTTTCCGTTA CCAAGAGAAC CCAAGATCAG CCTGGCTGCC AACAGCAAAC
    CCCATCCCAA ATTTCCAGTG TGGGACACTG AGCGATGACA GCCCGAATGA GAACCTGACC
    GAGAGAGTCC TACAGGACGC GCAGCGCTTG TTCCTGATGA ACGACGTGGT GCAGCCGGTC
    ACTGTAGATC CGTATGTGAC TCAGGACAGC ATTCGATTCT CTAAACTAGT GGTTGATATT
    GTTCAGGGGA AGGATACTCT CTACCACGTG ATGTACATTG GAACAGAGTA TGGCACCATC
    TTGAAGGCCC TTTCTACCAC TAATAGGAGC CTGAGGAGTT GTTATTTAGA GGAAATGCAG
    ATCCTCCCTG ATGGACAACG GGAAGCAATC AAGAGTCTCC AAATCCTGCA CAGCGACAGA
    TCGCTCTTTG TGGGCTTAAA TAACGGAGTG CTGAAAATTC CTCTGGAGAG ATGCTCCATG
    TACAGAACAG AAGGGGAATG CCTAGGAGCC AGAGACCCTT ACTGTGGCTG GGATAACAAG
    CAGAAGCGAT GCACCACCAT TGAGGACAGC TCCAACATGA GCCTATGGAC TCAAAATATT
    ACTGAGTGCC CAGTGAAAAA CCTGACAACA AATGGAAGAC TGGGGCCCTG GTCCCCATGG
    CAACCGTGTG AGCATTCAGA TGGGGACAGC ACAGGCTCCT GTATGTGTAG GTCACGTTCG
    TGTGACAGCC CTCGTCCTCG CTGTGGAGGT CGCAGCTGTG AAGGGGCCAG GATCGAGGTC
    GCAAATTGTT CAAGAAATGG CGCTTGGACA CCCTGGTCTT CCTGGGCCCT GTGCAGCACC
    TCCTGTGGGA TAGGCTTTCA GGTCCGCCAG CGGTCCTGCA GCAACCCTGC TCCACGGTAT
    GGGGGCAGGG TCTGTGTTGG ACAGAGCAGG GAAGAGAGAT TCTGTAATGA GAATAGTCCA
    TGTCCATTGC CAATTTTTTG GTCTTCGTGG GGTCCCTGGA ATAAATGTAG TGTGAATTGT
    GGTGGAGGAA TTCACTCAAG GCAAAGGACC TGTGAAAATG GAAATACATG TCCAGGCTGT
    GCTGTGGAAT ATAAGACCTG CAACCCAGAG AGCTGCCCAG AAGTGCGTAG GAACACACCC
    TGGACCCCTT GGATGCCTGT CAACATCACC CAGAATGGTG CCCGCCAGGA GCAGCGCTTC
    CGCTATACGT GTCGTGCCCA GCTGTCTGAT CCCCATGAGC TGCAGTTTGG GAGGAAGAAA
    ACTGAGAGTC GATTCTGTCC CAATGATGGC TCAGCTATGT GTGAAACTGA CTCTCTTGTT
    GACGACCTCC TCAAGACTGG TAAGACCTCT GCACGGATTA TCAATGGGGG CTGGTCCTTT
    TGGGGGGCCT GGTCTTCCTG TTCCAGGGAC TGTGAGTTGG GCTTTAGAAT CCGGAAAAGA
    ACATGCACAA ACCCTGAACC TAAAAATGGT GGCTTGCCTT GCGTGGGGTC AGCGATGGAG
    TACCAAGACT GCAACCCACA TCCTTGCCCA GTAAAAGGCT CATGGTCTTG CTGGACTCCT
    TGGTCTCAGT GCTCAGCAAC CTGTGGAGGA GGGCATTACC AGCGCACACG GACTTGCACC
    AACCCAGCTC CATCCAGTGG AGAGGATATC TGCATCGGGC TGCACACTGA GGAGGCTCTC
    TGCAACACCC ACCCATGTGA AGGTGGCTGG TCAGAGTGGT CAGAGTGGAG CCTGTGCAAT
    GAAGAGGGCA TCCAGTATCG CAGCCGTTAC TGTGAGGTAC ACAGCCCAGA CTCTTCTCAG
    TGCGTTGGAA ACAGCACACA ATACCAAGAT TGCCTGTATA ATGAAATTCC TGTGATCCTG
    CCAGCATCCA GCATTGATGA GAGCACAAAC TGTGGAGGTT TTAGTCTCAT CCATCTGATT
    GCCACTGGGG TCTCCTGCTT CTTTGGCTCT AGCCTCTTAA CGTTTGTGAT TTACGTGTAC
    TGCCAGCGCT GCCAGAGGCA GTCCCAGGAG TCGACCGTTA TCCACCCAAC CACTCCCAAC
    CATCTTCATT ACAAGGGCAA CACAACCCCC AAGAATGAGA AGTACACCCC CATGGAGTTC
    AAGACAGGAG ATGCGCTCAT ACCTTCCCTA AACGTGCTGT GCCTCGATCA CGAGGATGCT
    GTAGCAAACA AAGCCTGTGT TGACCCTCAA ATTCAAGCAG TGAGTTCCCT ACTGTACTGC
    ATGGCAAACA TTAAGGATGT GTGTAGGAGC ATTATAGGCC AGAGACAAGA AGTTGAAGCC
    AGGGACTCGT TGAAAAATGT TGTTAGAAAT GTGCGTCAAC CAAAGTTGTA TACTGTAAAG
    ATGAAGGAGA AAGCTTCATA CAATCATTGC TTCTTCCAAA ATGGGATTTT ATCCTTTTGC
    ATAGGAAAAG CCTGGCATAG CAATGGGTTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa11316
    Clone ID Related Accession (Same CDS sequence) XM_004942929.3 , XM_004942929.2
    Accession Version XM_004942929.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3297bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product semaphorin-5B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004942929.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGTTGTTT CAAGATTAAA GGCCATTTCC CTGTCATTGC CAAGCCTTTT TCTTCTTGCT 
    TTTCATCTCT CTGCATCACA GAATGTACCT GAGTACTCTG AAGCTGAACA TCAGCAGTGT
    GTCAGGAAAG AACACCCCAC AATCGCTTTT GAAGATCTGA AGCCTTGGGT GTCCAATTTC
    ACCTATCCAG GTGTGCATGA TTTTTCTCAG CTCGCCCTGG ATGCCAACAG GAACCAGCTC
    ATTGTGGGAG CAAGGAACTA CCTCTTCAGA CTTAGTCTAC ACAACGTTTC TCTTATTCAG
    GCAACAGCAT GGGGTTCAGA TGAAGACACC AGGAGGTCCT GTCAAAGTAA AGGGAAAACA
    GAGGAGGAGT GCCAGAACTA TGTCCGAGTG CTGATTGTTT ATGGCAAGAA GGTTTTTACC
    TGTGGTACCA ATGCCTTTTC ACCAGTGTGT TCCAGCCGTC AGGTAGGAAA TCTTAGCAAA
    ATCATTGACA GAATTAATGG AGTGGCTCGG TGCCCATATG ACCCCCGGCA TAACTCAACA
    GCTGTGATTA CATCACGAGG AGAACTCTAT GCTGCAACTG TAATTGATTT CTCTGGACGG
    GATCCTGCTA TTTACCGCAG CCTTGGAAAT GTGCCCCCAC TCAGGACAGC ACAGTACAAC
    TCCAAATGGC TCAATGAGCC TAACTTTATC GCTGCCTATG ACATCGGTTT GTTCACCTAC
    TTCTTCTTTC GAGAGAATGC GGTAGAACAC GATTGTGGGA AAACAGTATA TTCTCGTGTG
    GCCAGGGTCT GCAAAAACGA CATTGGAGGG AGATTTTTGC TGGAGGACAC GTGGACAACT
    TTCATGAAAG CCAGGCTGAA CTGCTCCAGA GCTGGAGAAA TCCCTTTCTA TTATAATGAA
    CTGCAAAGCA CCTTCTACCT TCCTGAACAA GACCTGATCT ATGGTGTCTT CACTACTAAT
    GTAAACAGCA TAGCTGCCTC AGCAGTGTGT GCCTTCAATC TCAGTGCCAT TACTCAGGCA
    TTTAATGGTC CTTTCCGTTA CCAAGAGAAC CCAAGATCAG CCTGGCTGCC AACAGCAAAC
    CCCATCCCAA ATTTCCAGTG TGGGACACTG AGCGATGACA GCCCGAATGA GAACCTGACC
    GAGAGAGTCC TACAGGACGC GCAGCGCTTG TTCCTGATGA ACGACGTGGT GCAGCCGGTC
    ACTGTAGATC CGTATGTGAC TCAGGACAGC ATTCGATTCT CTAAACTAGT GGTTGATATT
    GTTCAGGGGA AGGATACTCT CTACCACGTG ATGTACATTG GAACAGAGTA TGGCACCATC
    TTGAAGGCCC TTTCTACCAC TAATAGGAGC CTGAGGAGTT GTTATTTAGA GGAAATGCAG
    ATCCTCCCTG ATGGACAACG GGAAGCAATC AAGAGTCTCC AAATCCTGCA CAGCGACAGA
    TCGCTCTTTG TGGGCTTAAA TAACGGAGTG CTGAAAATTC CTCTGGAGAG ATGCTCCATG
    TACAGAACAG AAGGGGAATG CCTAGGAGCC AGAGACCCTT ACTGTGGCTG GGATAACAAG
    CAGAAGCGAT GCACCACCAT TGAGGACAGC TCCAACATGA GCCTATGGAC TCAAAATATT
    ACTGAGTGCC CAGTGAAAAA CCTGACAACA AATGGAAGAC TGGGGCCCTG GTCCCCATGG
    CAACCGTGTG AGCATTCAGA TGGGGACAGC ACAGGCTCCT GTATGTGTAG GTCACGTTCG
    TGTGACAGCC CTCGTCCTCG CTGTGGAGGT CGCAGCTGTG AAGGGGCCAG GATCGAGGTC
    GCAAATTGTT CAAGAAATGG CGCTTGGACA CCCTGGTCTT CCTGGGCCCT GTGCAGCACC
    TCCTGTGGGA TAGGCTTTCA GGTCCGCCAG CGGTCCTGCA GCAACCCTGC TCCACGGTAT
    GGGGGCAGGG TCTGTGTTGG ACAGAGCAGG GAAGAGAGAT TCTGTAATGA GAATAGTCCA
    TGTCCATTGC CAATTTTTTG GTCTTCGTGG GGTCCCTGGA ATAAATGTAG TGTGAATTGT
    GGTGGAGGAA TTCACTCAAG GCAAAGGACC TGTGAAAATG GAAATACATG TCCAGGCTGT
    GCTGTGGAAT ATAAGACCTG CAACCCAGAG AGCTGCCCAG AAGTGCGTAG GAACACACCC
    TGGACCCCTT GGATGCCTGT CAACATCACC CAGAATGGTG CCCGCCAGGA GCAGCGCTTC
    CGCTATACGT GTCGTGCCCA GCTGTCTGAT CCCCATGAGC TGCAGTTTGG GAGGAAGAAA
    ACTGAGAGTC GATTCTGTCC CAATGATGGC TCAGCTATGT GTGAAACTGA CTCTCTTGTT
    GACGACCTCC TCAAGACTGG TAAGACCTCT GCACGGATTA TCAATGGGGG CTGGTCCTTT
    TGGGGGGCCT GGTCTTCCTG TTCCAGGGAC TGTGAGTTGG GCTTTAGAAT CCGGAAAAGA
    ACATGCACAA ACCCTGAACC TAAAAATGGT GGCTTGCCTT GCGTGGGGTC AGCGATGGAG
    TACCAAGACT GCAACCCACA TCCTTGCCCA GTAAAAGGCT CATGGTCTTG CTGGACTCCT
    TGGTCTCAGT GCTCAGCAAC CTGTGGAGGA GGGCATTACC AGCGCACACG GACTTGCACC
    AACCCAGCTC CATCCAGTGG AGAGGATATC TGCATCGGGC TGCACACTGA GGAGGCTCTC
    TGCAACACCC ACCCATGTGA AGGTGGCTGG TCAGAGTGGT CAGAGTGGAG CCTGTGCAAT
    GAAGAGGGCA TCCAGTATCG CAGCCGTTAC TGTGAGGTAC ACAGCCCAGA CTCTTCTCAG
    TGCGTTGGAA ACAGCACACA ATACCAAGAT TGCCTGTATA ATGAAATTCC TGTGATCCTG
    CCAGCATCCA GCATTGATGA GAGCACAAAC TGTGGAGGTT TTAGTCTCAT CCATCTGATT
    GCCACTGGGG TCTCCTGCTT CTTTGGCTCT AGCCTCTTAA CGTTTGTGAT TTACGTGTAC
    TGCCAGCGCT GCCAGAGGCA GTCCCAGGAG TCGACCGTTA TCCACCCAAC CACTCCCAAC
    CATCTTCATT ACAAGGGCAA CACAACCCCC AAGAATGAGA AGTACACCCC CATGGAGTTC
    AAGACAGGAG ATGCGCTCAT ACCTTCCCTA AACGTGCTGT GCCTCGATCA CGAGGATGCT
    GTAGCAAACA AAGCCTGTGT TGACCCTCAA ATTCAAGCAC TGACAGAAGT GACACACTTA
    ATATCGGGGG GTCCAAACCT GGATATCTAC CAATGGAGAT GGAGATCAGA CACTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004942986.1
    CDS598..3894
    Translation

    Target ORF information:

    RefSeq Version XM_004942929.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus semaphorin 5B (SEMA5B), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004942929.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGTTGTTT CAAGATTAAA GGCCATTTCC CTGTCATTGC CAAGCCTTTT TCTTCTTGCT 
    TTTCATCTCT CTGCATCACA GAATGTACCT GAGTACTCTG AAGCTGAACA TCAGCAGTGT
    GTCAGGAAAG AACACCCCAC AATCGCTTTT GAAGATCTGA AGCCTTGGGT GTCCAATTTC
    ACCTATCCAG GTGTGCATGA TTTTTCTCAG CTCGCCCTGG ATGCCAACAG GAACCAGCTC
    ATTGTGGGAG CAAGGAACTA CCTCTTCAGA CTTAGTCTAC ACAACGTTTC TCTTATTCAG
    GCAACAGCAT GGGGTTCAGA TGAAGACACC AGGAGGTCCT GTCAAAGTAA AGGGAAAACA
    GAGGAGGAGT GCCAGAACTA TGTCCGAGTG CTGATTGTTT ATGGCAAGAA GGTTTTTACC
    TGTGGTACCA ATGCCTTTTC ACCAGTGTGT TCCAGCCGTC AGGTAGGAAA TCTTAGCAAA
    ATCATTGACA GAATTAATGG AGTGGCTCGG TGCCCATATG ACCCCCGGCA TAACTCAACA
    GCTGTGATTA CATCACGAGG AGAACTCTAT GCTGCAACTG TAATTGATTT CTCTGGACGG
    GATCCTGCTA TTTACCGCAG CCTTGGAAAT GTGCCCCCAC TCAGGACAGC ACAGTACAAC
    TCCAAATGGC TCAATGAGCC TAACTTTATC GCTGCCTATG ACATCGGTTT GTTCACCTAC
    TTCTTCTTTC GAGAGAATGC GGTAGAACAC GATTGTGGGA AAACAGTATA TTCTCGTGTG
    GCCAGGGTCT GCAAAAACGA CATTGGAGGG AGATTTTTGC TGGAGGACAC GTGGACAACT
    TTCATGAAAG CCAGGCTGAA CTGCTCCAGA GCTGGAGAAA TCCCTTTCTA TTATAATGAA
    CTGCAAAGCA CCTTCTACCT TCCTGAACAA GACCTGATCT ATGGTGTCTT CACTACTAAT
    GTAAACAGCA TAGCTGCCTC AGCAGTGTGT GCCTTCAATC TCAGTGCCAT TACTCAGGCA
    TTTAATGGTC CTTTCCGTTA CCAAGAGAAC CCAAGATCAG CCTGGCTGCC AACAGCAAAC
    CCCATCCCAA ATTTCCAGTG TGGGACACTG AGCGATGACA GCCCGAATGA GAACCTGACC
    GAGAGAGTCC TACAGGACGC GCAGCGCTTG TTCCTGATGA ACGACGTGGT GCAGCCGGTC
    ACTGTAGATC CGTATGTGAC TCAGGACAGC ATTCGATTCT CTAAACTAGT GGTTGATATT
    GTTCAGGGGA AGGATACTCT CTACCACGTG ATGTACATTG GAACAGAGTA TGGCACCATC
    TTGAAGGCCC TTTCTACCAC TAATAGGAGC CTGAGGAGTT GTTATTTAGA GGAAATGCAG
    ATCCTCCCTG ATGGACAACG GGAAGCAATC AAGAGTCTCC AAATCCTGCA CAGCGACAGA
    TCGCTCTTTG TGGGCTTAAA TAACGGAGTG CTGAAAATTC CTCTGGAGAG ATGCTCCATG
    TACAGAACAG AAGGGGAATG CCTAGGAGCC AGAGACCCTT ACTGTGGCTG GGATAACAAG
    CAGAAGCGAT GCACCACCAT TGAGGACAGC TCCAACATGA GCCTATGGAC TCAAAATATT
    ACTGAGTGCC CAGTGAAAAA CCTGACAACA AATGGAAGAC TGGGGCCCTG GTCCCCATGG
    CAACCGTGTG AGCATTCAGA TGGGGACAGC ACAGGCTCCT GTATGTGTAG GTCACGTTCG
    TGTGACAGCC CTCGTCCTCG CTGTGGAGGT CGCAGCTGTG AAGGGGCCAG GATCGAGGTC
    GCAAATTGTT CAAGAAATGG CGCTTGGACA CCCTGGTCTT CCTGGGCCCT GTGCAGCACC
    TCCTGTGGGA TAGGCTTTCA GGTCCGCCAG CGGTCCTGCA GCAACCCTGC TCCACGGTAT
    GGGGGCAGGG TCTGTGTTGG ACAGAGCAGG GAAGAGAGAT TCTGTAATGA GAATAGTCCA
    TGTCCATTGC CAATTTTTTG GTCTTCGTGG GGTCCCTGGA ATAAATGTAG TGTGAATTGT
    GGTGGAGGAA TTCACTCAAG GCAAAGGACC TGTGAAAATG GAAATACATG TCCAGGCTGT
    GCTGTGGAAT ATAAGACCTG CAACCCAGAG AGCTGCCCAG AAGTGCGTAG GAACACACCC
    TGGACCCCTT GGATGCCTGT CAACATCACC CAGAATGGTG CCCGCCAGGA GCAGCGCTTC
    CGCTATACGT GTCGTGCCCA GCTGTCTGAT CCCCATGAGC TGCAGTTTGG GAGGAAGAAA
    ACTGAGAGTC GATTCTGTCC CAATGATGGC TCAGCTATGT GTGAAACTGA CTCTCTTGTT
    GACGACCTCC TCAAGACTGG TAAGACCTCT GCACGGATTA TCAATGGGGG CTGGTCCTTT
    TGGGGGGCCT GGTCTTCCTG TTCCAGGGAC TGTGAGTTGG GCTTTAGAAT CCGGAAAAGA
    ACATGCACAA ACCCTGAACC TAAAAATGGT GGCTTGCCTT GCGTGGGGTC AGCGATGGAG
    TACCAAGACT GCAACCCACA TCCTTGCCCA GTAAAAGGCT CATGGTCTTG CTGGACTCCT
    TGGTCTCAGT GCTCAGCAAC CTGTGGAGGA GGGCATTACC AGCGCACACG GACTTGCACC
    AACCCAGCTC CATCCAGTGG AGAGGATATC TGCATCGGGC TGCACACTGA GGAGGCTCTC
    TGCAACACCC ACCCATGTGA AGGTGGCTGG TCAGAGTGGT CAGAGTGGAG CCTGTGCAAT
    GAAGAGGGCA TCCAGTATCG CAGCCGTTAC TGTGAGGTAC ACAGCCCAGA CTCTTCTCAG
    TGCGTTGGAA ACAGCACACA ATACCAAGAT TGCCTGTATA ATGAAATTCC TGTGATCCTG
    CCAGCATCCA GCATTGATGA GAGCACAAAC TGTGGAGGTT TTAGTCTCAT CCATCTGATT
    GCCACTGGGG TCTCCTGCTT CTTTGGCTCT AGCCTCTTAA CGTTTGTGAT TTACGTGTAC
    TGCCAGCGCT GCCAGAGGCA GTCCCAGGAG TCGACCGTTA TCCACCCAAC CACTCCCAAC
    CATCTTCATT ACAAGGGCAA CACAACCCCC AAGAATGAGA AGTACACCCC CATGGAGTTC
    AAGACAGGAG ATGCGCTCAT ACCTTCCCTA AACGTGCTGT GCCTCGATCA CGAGGATGCT
    GTAGCAAACA AAGCCTGTGT TGACCCTCAA ATTCAAGCAC TGACAGAAGT GACACACTTA
    ATATCGGGGG GTCCAAACCT GGATATCTAC CAATGGAGAT GGAGATCAGA CACTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa11316
    Clone ID Related Accession (Same CDS sequence) XM_004942929.3 , XM_004942929.2
    Accession Version XM_004942929.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3297bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product semaphorin-5B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004942929.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGTTGTTT CAAGATTAAA GGCCATTTCC CTGTCATTGC CAAGCCTTTT TCTTCTTGCT 
    TTTCATCTCT CTGCATCACA GAATGTACCT GAGTACTCTG AAGCTGAACA TCAGCAGTGT
    GTCAGGAAAG AACACCCCAC AATCGCTTTT GAAGATCTGA AGCCTTGGGT GTCCAATTTC
    ACCTATCCAG GTGTGCATGA TTTTTCTCAG CTCGCCCTGG ATGCCAACAG GAACCAGCTC
    ATTGTGGGAG CAAGGAACTA CCTCTTCAGA CTTAGTCTAC ACAACGTTTC TCTTATTCAG
    GCAACAGCAT GGGGTTCAGA TGAAGACACC AGGAGGTCCT GTCAAAGTAA AGGGAAAACA
    GAGGAGGAGT GCCAGAACTA TGTCCGAGTG CTGATTGTTT ATGGCAAGAA GGTTTTTACC
    TGTGGTACCA ATGCCTTTTC ACCAGTGTGT TCCAGCCGTC AGGTAGGAAA TCTTAGCAAA
    ATCATTGACA GAATTAATGG AGTGGCTCGG TGCCCATATG ACCCCCGGCA TAACTCAACA
    GCTGTGATTA CATCACGAGG AGAACTCTAT GCTGCAACTG TAATTGATTT CTCTGGACGG
    GATCCTGCTA TTTACCGCAG CCTTGGAAAT GTGCCCCCAC TCAGGACAGC ACAGTACAAC
    TCCAAATGGC TCAATGAGCC TAACTTTATC GCTGCCTATG ACATCGGTTT GTTCACCTAC
    TTCTTCTTTC GAGAGAATGC GGTAGAACAC GATTGTGGGA AAACAGTATA TTCTCGTGTG
    GCCAGGGTCT GCAAAAACGA CATTGGAGGG AGATTTTTGC TGGAGGACAC GTGGACAACT
    TTCATGAAAG CCAGGCTGAA CTGCTCCAGA GCTGGAGAAA TCCCTTTCTA TTATAATGAA
    CTGCAAAGCA CCTTCTACCT TCCTGAACAA GACCTGATCT ATGGTGTCTT CACTACTAAT
    GTAAACAGCA TAGCTGCCTC AGCAGTGTGT GCCTTCAATC TCAGTGCCAT TACTCAGGCA
    TTTAATGGTC CTTTCCGTTA CCAAGAGAAC CCAAGATCAG CCTGGCTGCC AACAGCAAAC
    CCCATCCCAA ATTTCCAGTG TGGGACACTG AGCGATGACA GCCCGAATGA GAACCTGACC
    GAGAGAGTCC TACAGGACGC GCAGCGCTTG TTCCTGATGA ACGACGTGGT GCAGCCGGTC
    ACTGTAGATC CGTATGTGAC TCAGGACAGC ATTCGATTCT CTAAACTAGT GGTTGATATT
    GTTCAGGGGA AGGATACTCT CTACCACGTG ATGTACATTG GAACAGAGTA TGGCACCATC
    TTGAAGGCCC TTTCTACCAC TAATAGGAGC CTGAGGAGTT GTTATTTAGA GGAAATGCAG
    ATCCTCCCTG ATGGACAACG GGAAGCAATC AAGAGTCTCC AAATCCTGCA CAGCGACAGA
    TCGCTCTTTG TGGGCTTAAA TAACGGAGTG CTGAAAATTC CTCTGGAGAG ATGCTCCATG
    TACAGAACAG AAGGGGAATG CCTAGGAGCC AGAGACCCTT ACTGTGGCTG GGATAACAAG
    CAGAAGCGAT GCACCACCAT TGAGGACAGC TCCAACATGA GCCTATGGAC TCAAAATATT
    ACTGAGTGCC CAGTGAAAAA CCTGACAACA AATGGAAGAC TGGGGCCCTG GTCCCCATGG
    CAACCGTGTG AGCATTCAGA TGGGGACAGC ACAGGCTCCT GTATGTGTAG GTCACGTTCG
    TGTGACAGCC CTCGTCCTCG CTGTGGAGGT CGCAGCTGTG AAGGGGCCAG GATCGAGGTC
    GCAAATTGTT CAAGAAATGG CGCTTGGACA CCCTGGTCTT CCTGGGCCCT GTGCAGCACC
    TCCTGTGGGA TAGGCTTTCA GGTCCGCCAG CGGTCCTGCA GCAACCCTGC TCCACGGTAT
    GGGGGCAGGG TCTGTGTTGG ACAGAGCAGG GAAGAGAGAT TCTGTAATGA GAATAGTCCA
    TGTCCATTGC CAATTTTTTG GTCTTCGTGG GGTCCCTGGA ATAAATGTAG TGTGAATTGT
    GGTGGAGGAA TTCACTCAAG GCAAAGGACC TGTGAAAATG GAAATACATG TCCAGGCTGT
    GCTGTGGAAT ATAAGACCTG CAACCCAGAG AGCTGCCCAG AAGTGCGTAG GAACACACCC
    TGGACCCCTT GGATGCCTGT CAACATCACC CAGAATGGTG CCCGCCAGGA GCAGCGCTTC
    CGCTATACGT GTCGTGCCCA GCTGTCTGAT CCCCATGAGC TGCAGTTTGG GAGGAAGAAA
    ACTGAGAGTC GATTCTGTCC CAATGATGGC TCAGCTATGT GTGAAACTGA CTCTCTTGTT
    GACGACCTCC TCAAGACTGG TAAGACCTCT GCACGGATTA TCAATGGGGG CTGGTCCTTT
    TGGGGGGCCT GGTCTTCCTG TTCCAGGGAC TGTGAGTTGG GCTTTAGAAT CCGGAAAAGA
    ACATGCACAA ACCCTGAACC TAAAAATGGT GGCTTGCCTT GCGTGGGGTC AGCGATGGAG
    TACCAAGACT GCAACCCACA TCCTTGCCCA GTAAAAGGCT CATGGTCTTG CTGGACTCCT
    TGGTCTCAGT GCTCAGCAAC CTGTGGAGGA GGGCATTACC AGCGCACACG GACTTGCACC
    AACCCAGCTC CATCCAGTGG AGAGGATATC TGCATCGGGC TGCACACTGA GGAGGCTCTC
    TGCAACACCC ACCCATGTGA AGGTGGCTGG TCAGAGTGGT CAGAGTGGAG CCTGTGCAAT
    GAAGAGGGCA TCCAGTATCG CAGCCGTTAC TGTGAGGTAC ACAGCCCAGA CTCTTCTCAG
    TGCGTTGGAA ACAGCACACA ATACCAAGAT TGCCTGTATA ATGAAATTCC TGTGATCCTG
    CCAGCATCCA GCATTGATGA GAGCACAAAC TGTGGAGGTT TTAGTCTCAT CCATCTGATT
    GCCACTGGGG TCTCCTGCTT CTTTGGCTCT AGCCTCTTAA CGTTTGTGAT TTACGTGTAC
    TGCCAGCGCT GCCAGAGGCA GTCCCAGGAG TCGACCGTTA TCCACCCAAC CACTCCCAAC
    CATCTTCATT ACAAGGGCAA CACAACCCCC AAGAATGAGA AGTACACCCC CATGGAGTTC
    AAGACAGGAG ATGCGCTCAT ACCTTCCCTA AACGTGCTGT GCCTCGATCA CGAGGATGCT
    GTAGCAAACA AAGCCTGTGT TGACCCTCAA ATTCAAGCAC TGACAGAAGT GACACACTTA
    ATATCGGGGG GTCCAAACCT GGATATCTAC CAATGGAGAT GGAGATCAGA CACTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004942986.1
    CDS607..3903
    Translation

    Target ORF information:

    RefSeq Version XM_004942929.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (SEMA5B), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004942929.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGTTGTTT CAAGATTAAA GGCCATTTCC CTGTCATTGC CAAGCCTTTT TCTTCTTGCT 
    TTTCATCTCT CTGCATCACA GAATGTACCT GAGTACTCTG AAGCTGAACA TCAGCAGTGT
    GTCAGGAAAG AACACCCCAC AATCGCTTTT GAAGATCTGA AGCCTTGGGT GTCCAATTTC
    ACCTATCCAG GTGTGCATGA TTTTTCTCAG CTCGCCCTGG ATGCCAACAG GAACCAGCTC
    ATTGTGGGAG CAAGGAACTA CCTCTTCAGA CTTAGTCTAC ACAACGTTTC TCTTATTCAG
    GCAACAGCAT GGGGTTCAGA TGAAGACACC AGGAGGTCCT GTCAAAGTAA AGGGAAAACA
    GAGGAGGAGT GCCAGAACTA TGTCCGAGTG CTGATTGTTT ATGGCAAGAA GGTTTTTACC
    TGTGGTACCA ATGCCTTTTC ACCAGTGTGT TCCAGCCGTC AGGTAGGAAA TCTTAGCAAA
    ATCATTGACA GAATTAATGG AGTGGCTCGG TGCCCATATG ACCCCCGGCA TAACTCAACA
    GCTGTGATTA CATCACGAGG AGAACTCTAT GCTGCAACTG TAATTGATTT CTCTGGACGG
    GATCCTGCTA TTTACCGCAG CCTTGGAAAT GTGCCCCCAC TCAGGACAGC ACAGTACAAC
    TCCAAATGGC TCAATGAGCC TAACTTTATC GCTGCCTATG ACATCGGTTT GTTCACCTAC
    TTCTTCTTTC GAGAGAATGC GGTAGAACAC GATTGTGGGA AAACAGTATA TTCTCGTGTG
    GCCAGGGTCT GCAAAAACGA CATTGGAGGG AGATTTTTGC TGGAGGACAC GTGGACAACT
    TTCATGAAAG CCAGGCTGAA CTGCTCCAGA GCTGGAGAAA TCCCTTTCTA TTATAATGAA
    CTGCAAAGCA CCTTCTACCT TCCTGAACAA GACCTGATCT ATGGTGTCTT CACTACTAAT
    GTAAACAGCA TAGCTGCCTC AGCAGTGTGT GCCTTCAATC TCAGTGCCAT TACTCAGGCA
    TTTAATGGTC CTTTCCGTTA CCAAGAGAAC CCAAGATCAG CCTGGCTGCC AACAGCAAAC
    CCCATCCCAA ATTTCCAGTG TGGGACACTG AGCGATGACA GCCCGAATGA GAACCTGACC
    GAGAGAGTCC TACAGGACGC GCAGCGCTTG TTCCTGATGA ACGACGTGGT GCAGCCGGTC
    ACTGTAGATC CGTATGTGAC TCAGGACAGC ATTCGATTCT CTAAACTAGT GGTTGATATT
    GTTCAGGGGA AGGATACTCT CTACCACGTG ATGTACATTG GAACAGAGTA TGGCACCATC
    TTGAAGGCCC TTTCTACCAC TAATAGGAGC CTGAGGAGTT GTTATTTAGA GGAAATGCAG
    ATCCTCCCTG ATGGACAACG GGAAGCAATC AAGAGTCTCC AAATCCTGCA CAGCGACAGA
    TCGCTCTTTG TGGGCTTAAA TAACGGAGTG CTGAAAATTC CTCTGGAGAG ATGCTCCATG
    TACAGAACAG AAGGGGAATG CCTAGGAGCC AGAGACCCTT ACTGTGGCTG GGATAACAAG
    CAGAAGCGAT GCACCACCAT TGAGGACAGC TCCAACATGA GCCTATGGAC TCAAAATATT
    ACTGAGTGCC CAGTGAAAAA CCTGACAACA AATGGAAGAC TGGGGCCCTG GTCCCCATGG
    CAACCGTGTG AGCATTCAGA TGGGGACAGC ACAGGCTCCT GTATGTGTAG GTCACGTTCG
    TGTGACAGCC CTCGTCCTCG CTGTGGAGGT CGCAGCTGTG AAGGGGCCAG GATCGAGGTC
    GCAAATTGTT CAAGAAATGG CGCTTGGACA CCCTGGTCTT CCTGGGCCCT GTGCAGCACC
    TCCTGTGGGA TAGGCTTTCA GGTCCGCCAG CGGTCCTGCA GCAACCCTGC TCCACGGTAT
    GGGGGCAGGG TCTGTGTTGG ACAGAGCAGG GAAGAGAGAT TCTGTAATGA GAATAGTCCA
    TGTCCATTGC CAATTTTTTG GTCTTCGTGG GGTCCCTGGA ATAAATGTAG TGTGAATTGT
    GGTGGAGGAA TTCACTCAAG GCAAAGGACC TGTGAAAATG GAAATACATG TCCAGGCTGT
    GCTGTGGAAT ATAAGACCTG CAACCCAGAG AGCTGCCCAG AAGTGCGTAG GAACACACCC
    TGGACCCCTT GGATGCCTGT CAACATCACC CAGAATGGTG CCCGCCAGGA GCAGCGCTTC
    CGCTATACGT GTCGTGCCCA GCTGTCTGAT CCCCATGAGC TGCAGTTTGG GAGGAAGAAA
    ACTGAGAGTC GATTCTGTCC CAATGATGGC TCAGCTATGT GTGAAACTGA CTCTCTTGTT
    GACGACCTCC TCAAGACTGG TAAGACCTCT GCACGGATTA TCAATGGGGG CTGGTCCTTT
    TGGGGGGCCT GGTCTTCCTG TTCCAGGGAC TGTGAGTTGG GCTTTAGAAT CCGGAAAAGA
    ACATGCACAA ACCCTGAACC TAAAAATGGT GGCTTGCCTT GCGTGGGGTC AGCGATGGAG
    TACCAAGACT GCAACCCACA TCCTTGCCCA GTAAAAGGCT CATGGTCTTG CTGGACTCCT
    TGGTCTCAGT GCTCAGCAAC CTGTGGAGGA GGGCATTACC AGCGCACACG GACTTGCACC
    AACCCAGCTC CATCCAGTGG AGAGGATATC TGCATCGGGC TGCACACTGA GGAGGCTCTC
    TGCAACACCC ACCCATGTGA AGGTGGCTGG TCAGAGTGGT CAGAGTGGAG CCTGTGCAAT
    GAAGAGGGCA TCCAGTATCG CAGCCGTTAC TGTGAGGTAC ACAGCCCAGA CTCTTCTCAG
    TGCGTTGGAA ACAGCACACA ATACCAAGAT TGCCTGTATA ATGAAATTCC TGTGATCCTG
    CCAGCATCCA GCATTGATGA GAGCACAAAC TGTGGAGGTT TTAGTCTCAT CCATCTGATT
    GCCACTGGGG TCTCCTGCTT CTTTGGCTCT AGCCTCTTAA CGTTTGTGAT TTACGTGTAC
    TGCCAGCGCT GCCAGAGGCA GTCCCAGGAG TCGACCGTTA TCCACCCAAC CACTCCCAAC
    CATCTTCATT ACAAGGGCAA CACAACCCCC AAGAATGAGA AGTACACCCC CATGGAGTTC
    AAGACAGGAG ATGCGCTCAT ACCTTCCCTA AACGTGCTGT GCCTCGATCA CGAGGATGCT
    GTAGCAAACA AAGCCTGTGT TGACCCTCAA ATTCAAGCAC TGACAGAAGT GACACACTTA
    ATATCGGGGG GTCCAAACCT GGATATCTAC CAATGGAGAT GGAGATCAGA CACTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa11317
    Clone ID Related Accession (Same CDS sequence) XM_015289991.2 , XM_015289991.1
    Accession Version XM_015289991.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3279bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product semaphorin-5B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289991.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGTTGTTT CAAGATTAAA GGCCATTTCC CTGTCATTGC CAAGCCTTTT TCTTCTTGCT 
    TTTCATCTCT CTGCATCACA GAATGTACCT GAGTACTCTG AAGCTGAACA TCAGCAGTGT
    GTCAGGAAAG AACACCCCAC AATCGCTTTT GAAGATCTGA AGCCTTGGGT GTCCAATTTC
    ACCTATCCAG GTGTGCATGA TTTTTCTCAG CTCGCCCTGG ATGCCAACAG GAACCAGCTC
    ATTGTGGGAG CAAGGAACTA CCTCTTCAGA CTTAGTCTAC ACAACGTTTC TCTTATTCAG
    GCAACAGCAT GGGGTTCAGA TGAAGACACC AGGAGGTCCT GTCAAAGTAA AGGGAAAACA
    GAGGAGGAGT GCCAGAACTA TGTCCGAGTG CTGATTGTTT ATGGCAAGAA GGTTTTTACC
    TGTGGTACCA ATGCCTTTTC ACCAGTGTGT TCCAGCCGTC AGGTAGGAAA TCTTAGCAAA
    ATCATTGACA GAATTAATGG AGTGGCTCGG TGCCCATATG ACCCCCGGCA TAACTCAACA
    GCTGTGATTA CATCACGAGG AGAACTCTAT GCTGCAACTG TAATTGATTT CTCTGGACGG
    GATCCTGCTA TTTACCGCAG CCTTGGAAAT GTGCCCCCAC TCAGGACAGC ACAGTACAAC
    TCCAAATGGC TCAATGAGCC TAACTTTATC GCTGCCTATG ACATCGGTTT GTTCACCTAC
    TTCTTCTTTC GAGAGAATGC GGTAGAACAC GATTGTGGGA AAACAGTATA TTCTCGTGTG
    GCCAGGGTCT GCAAAAACGA CATTGGAGGG AGATTTTTGC TGGAGGACAC GTGGACAACT
    TTCATGAAAG CCAGGCTGAA CTGCTCCAGA GCTGGAGAAA TCCCTTTCTA TTATAATGAA
    CTGCAAAGCA CCTTCTACCT TCCTGAACAA GACCTGATCT ATGGTGTCTT CACTACTAAT
    GTAAACAGCA TAGCTGCCTC AGCAGTGTGT GCCTTCAATC TCAGTGCCAT TACTCAGGCA
    TTTAATGGTC CTTTCCGTTA CCAAGAGAAC CCAAGATCAG CCTGGCTGCC AACAGCAAAC
    CCCATCCCAA ATTTCCAGTG TGGGACACTG AGCGATGACA GCCCGAATGA GAACCTGACC
    GAGAGAGTCC TACAGGACGC GCAGCGCTTG TTCCTGATGA ACGACGTGGT GCAGCCGGTC
    ACTGTAGATC CGTATGTGAC TCAGGACAGC ATTCGATTCT CTAAACTAGT GGTTGATATT
    GTTCAGGGGA AGGATACTCT CTACCACGTG ATGTACATTG GAACAGAGTA TGGCACCATC
    TTGAAGGCCC TTTCTACCAC TAATAGGAGC CTGAGGAGTT GTTATTTAGA GGAAATGCAG
    ATCCTCCCTG ATGGACAACG GGAAGCAATC AAGAGTCTCC AAATCCTGCA CAGCGACAGA
    TCGCTCTTTG TGGGCTTAAA TAACGGAGTG CTGAAAATTC CTCTGGAGAG ATGCTCCATG
    TACAGAACAG AAGGGGAATG CCTAGGAGCC AGAGACCCTT ACTGTGGCTG GGATAACAAG
    CAGAAGCGAT GCACCACCAT TGAGGACAGC TCCAACATGA GCCTATGGAC TCAAAATATT
    ACTGAGTGCC CAGTGAAAAA CCTGACAACA AATGGAAGAC TGGGGCCCTG GTCCCCATGG
    CAACCGTGTG AGCATTCAGA TGGGGACAGC ACAGGCTCCT GTATGTGTAG GTCACGTTCG
    TGTGACAGCC CTCGTCCTCG CTGTGGAGGT CGCAGCTGTG AAGGGGCCAG GATCGAGGTC
    GCAAATTGTT CAAGAAATGG CGCTTGGACA CCCTGGTCTT CCTGGGCCCT GTGCAGCACC
    TCCTGTGGGA TAGGCTTTCA GGTCCGCCAG CGGTCCTGCA GCAACCCTGC TCCACGGTAT
    GGGGGCAGGG TCTGTGTTGG ACAGAGCAGG GAAGAGAGAT TCTGTAATGA GAATAGTCCA
    TGTCCATTGC CAATTTTTTG GTCTTCGTGG GGTCCCTGGA ATAAATGTAG TGTGAATTGT
    GGTGGAGGAA TTCACTCAAG GCAAAGGACC TGTGAAAATG GAAATACATG TCCAGGCTGT
    GCTGTGGAAT ATAAGACCTG CAACCCAGAG AGCTGCCCAG AAGTGCGTAG GAACACACCC
    TGGACCCCTT GGATGCCTGT CAACATCACC CAGAATGGTG CCCGCCAGGA GCAGCGCTTC
    CGCTATACGT GTCGTGCCCA GCTGTCTGAT CCCCATGAGC TGCAGTTTGG GAGGAAGAAA
    ACTGAGAGTC GATTCTGTCC CAATGATGGC TCAGCTATGT GTGAAACTGA CTCTCTTGTT
    GACGACCTCC TCAAGACTGG TAAGACCTCT GCACGGATTA TCAATGGGGG CTGGTCCTTT
    TGGGGGGCCT GGTCTTCCTG TTCCAGGGAC TGTGAGTTGG GCTTTAGAAT CCGGAAAAGA
    ACATGCACAA ACCCTGAACC TAAAAATGGT GGCTTGCCTT GCGTGGGGTC AGCGATGGAG
    TACCAAGACT GCAACCCACA TCCTTGCCCA GTAAAAGGCT CATGGTCTTG CTGGACTCCT
    TGGTCTCAGT GCTCAGCAAC CTGTGGAGGA GGGCATTACC AGCGCACACG GACTTGCACC
    AACCCAGCTC CATCCAGTGG AGAGGATATC TGCATCGGGC TGCACACTGA GGAGGCTCTC
    TGCAACACCC ACCCATGTGA AGGTGGCTGG TCAGAGTGGT CAGAGTGGAG CCTGTGCAAT
    GAAGAGGGCA TCCAGTATCG CAGCCGTTAC TGTGAGGTAC ACAGCCCAGA CTCTTCTCAG
    TGCGTTGGAA ACAGCACACA ATACCAAGAT TGCCTGTATA ATGAAATTCC TGTGATCCTG
    CCAGCATCCA GCATTGATGA GAGCACAAAC TGTGGAGGTT TTAGTCTCAT CCATCTGATT
    GCCACTGGGG TCTCCTGCTT CTTTGGCTCT AGCCTCTTAA CGTTTGTGAT TTACGTGTAC
    TGCCAGCGCT GCCAGAGGCA GTCCCAGGAG TCGACCGTTA TCCACCCAAC CACTCCCAAC
    CATCTTCATT ACAAGGGCAA CACAACCCCC AAGAATGAGA AGTACACCCC CATGGAGTTC
    AAGACACTTA ACAAGAACAA CTTGATACCA GACGATAGGA CCAACTTCTA CCCCTTGCAG
    CAAACCAACG TGTACACGAC AACCTACTAT CCCAGTACAC TGAATAAATA TGACTACAGG
    CCCGAGGCCT CCCCTGGAAG GACCTTTACC AACAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145477.1
    CDS597..3875
    Translation

    Target ORF information:

    RefSeq Version XM_015289991.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus semaphorin 5B (SEMA5B), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289991.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGTTGTTT CAAGATTAAA GGCCATTTCC CTGTCATTGC CAAGCCTTTT TCTTCTTGCT 
    TTTCATCTCT CTGCATCACA GAATGTACCT GAGTACTCTG AAGCTGAACA TCAGCAGTGT
    GTCAGGAAAG AACACCCCAC AATCGCTTTT GAAGATCTGA AGCCTTGGGT GTCCAATTTC
    ACCTATCCAG GTGTGCATGA TTTTTCTCAG CTCGCCCTGG ATGCCAACAG GAACCAGCTC
    ATTGTGGGAG CAAGGAACTA CCTCTTCAGA CTTAGTCTAC ACAACGTTTC TCTTATTCAG
    GCAACAGCAT GGGGTTCAGA TGAAGACACC AGGAGGTCCT GTCAAAGTAA AGGGAAAACA
    GAGGAGGAGT GCCAGAACTA TGTCCGAGTG CTGATTGTTT ATGGCAAGAA GGTTTTTACC
    TGTGGTACCA ATGCCTTTTC ACCAGTGTGT TCCAGCCGTC AGGTAGGAAA TCTTAGCAAA
    ATCATTGACA GAATTAATGG AGTGGCTCGG TGCCCATATG ACCCCCGGCA TAACTCAACA
    GCTGTGATTA CATCACGAGG AGAACTCTAT GCTGCAACTG TAATTGATTT CTCTGGACGG
    GATCCTGCTA TTTACCGCAG CCTTGGAAAT GTGCCCCCAC TCAGGACAGC ACAGTACAAC
    TCCAAATGGC TCAATGAGCC TAACTTTATC GCTGCCTATG ACATCGGTTT GTTCACCTAC
    TTCTTCTTTC GAGAGAATGC GGTAGAACAC GATTGTGGGA AAACAGTATA TTCTCGTGTG
    GCCAGGGTCT GCAAAAACGA CATTGGAGGG AGATTTTTGC TGGAGGACAC GTGGACAACT
    TTCATGAAAG CCAGGCTGAA CTGCTCCAGA GCTGGAGAAA TCCCTTTCTA TTATAATGAA
    CTGCAAAGCA CCTTCTACCT TCCTGAACAA GACCTGATCT ATGGTGTCTT CACTACTAAT
    GTAAACAGCA TAGCTGCCTC AGCAGTGTGT GCCTTCAATC TCAGTGCCAT TACTCAGGCA
    TTTAATGGTC CTTTCCGTTA CCAAGAGAAC CCAAGATCAG CCTGGCTGCC AACAGCAAAC
    CCCATCCCAA ATTTCCAGTG TGGGACACTG AGCGATGACA GCCCGAATGA GAACCTGACC
    GAGAGAGTCC TACAGGACGC GCAGCGCTTG TTCCTGATGA ACGACGTGGT GCAGCCGGTC
    ACTGTAGATC CGTATGTGAC TCAGGACAGC ATTCGATTCT CTAAACTAGT GGTTGATATT
    GTTCAGGGGA AGGATACTCT CTACCACGTG ATGTACATTG GAACAGAGTA TGGCACCATC
    TTGAAGGCCC TTTCTACCAC TAATAGGAGC CTGAGGAGTT GTTATTTAGA GGAAATGCAG
    ATCCTCCCTG ATGGACAACG GGAAGCAATC AAGAGTCTCC AAATCCTGCA CAGCGACAGA
    TCGCTCTTTG TGGGCTTAAA TAACGGAGTG CTGAAAATTC CTCTGGAGAG ATGCTCCATG
    TACAGAACAG AAGGGGAATG CCTAGGAGCC AGAGACCCTT ACTGTGGCTG GGATAACAAG
    CAGAAGCGAT GCACCACCAT TGAGGACAGC TCCAACATGA GCCTATGGAC TCAAAATATT
    ACTGAGTGCC CAGTGAAAAA CCTGACAACA AATGGAAGAC TGGGGCCCTG GTCCCCATGG
    CAACCGTGTG AGCATTCAGA TGGGGACAGC ACAGGCTCCT GTATGTGTAG GTCACGTTCG
    TGTGACAGCC CTCGTCCTCG CTGTGGAGGT CGCAGCTGTG AAGGGGCCAG GATCGAGGTC
    GCAAATTGTT CAAGAAATGG CGCTTGGACA CCCTGGTCTT CCTGGGCCCT GTGCAGCACC
    TCCTGTGGGA TAGGCTTTCA GGTCCGCCAG CGGTCCTGCA GCAACCCTGC TCCACGGTAT
    GGGGGCAGGG TCTGTGTTGG ACAGAGCAGG GAAGAGAGAT TCTGTAATGA GAATAGTCCA
    TGTCCATTGC CAATTTTTTG GTCTTCGTGG GGTCCCTGGA ATAAATGTAG TGTGAATTGT
    GGTGGAGGAA TTCACTCAAG GCAAAGGACC TGTGAAAATG GAAATACATG TCCAGGCTGT
    GCTGTGGAAT ATAAGACCTG CAACCCAGAG AGCTGCCCAG AAGTGCGTAG GAACACACCC
    TGGACCCCTT GGATGCCTGT CAACATCACC CAGAATGGTG CCCGCCAGGA GCAGCGCTTC
    CGCTATACGT GTCGTGCCCA GCTGTCTGAT CCCCATGAGC TGCAGTTTGG GAGGAAGAAA
    ACTGAGAGTC GATTCTGTCC CAATGATGGC TCAGCTATGT GTGAAACTGA CTCTCTTGTT
    GACGACCTCC TCAAGACTGG TAAGACCTCT GCACGGATTA TCAATGGGGG CTGGTCCTTT
    TGGGGGGCCT GGTCTTCCTG TTCCAGGGAC TGTGAGTTGG GCTTTAGAAT CCGGAAAAGA
    ACATGCACAA ACCCTGAACC TAAAAATGGT GGCTTGCCTT GCGTGGGGTC AGCGATGGAG
    TACCAAGACT GCAACCCACA TCCTTGCCCA GTAAAAGGCT CATGGTCTTG CTGGACTCCT
    TGGTCTCAGT GCTCAGCAAC CTGTGGAGGA GGGCATTACC AGCGCACACG GACTTGCACC
    AACCCAGCTC CATCCAGTGG AGAGGATATC TGCATCGGGC TGCACACTGA GGAGGCTCTC
    TGCAACACCC ACCCATGTGA AGGTGGCTGG TCAGAGTGGT CAGAGTGGAG CCTGTGCAAT
    GAAGAGGGCA TCCAGTATCG CAGCCGTTAC TGTGAGGTAC ACAGCCCAGA CTCTTCTCAG
    TGCGTTGGAA ACAGCACACA ATACCAAGAT TGCCTGTATA ATGAAATTCC TGTGATCCTG
    CCAGCATCCA GCATTGATGA GAGCACAAAC TGTGGAGGTT TTAGTCTCAT CCATCTGATT
    GCCACTGGGG TCTCCTGCTT CTTTGGCTCT AGCCTCTTAA CGTTTGTGAT TTACGTGTAC
    TGCCAGCGCT GCCAGAGGCA GTCCCAGGAG TCGACCGTTA TCCACCCAAC CACTCCCAAC
    CATCTTCATT ACAAGGGCAA CACAACCCCC AAGAATGAGA AGTACACCCC CATGGAGTTC
    AAGACACTTA ACAAGAACAA CTTGATACCA GACGATAGGA CCAACTTCTA CCCCTTGCAG
    CAAACCAACG TGTACACGAC AACCTACTAT CCCAGTACAC TGAATAAATA TGACTACAGG
    CCCGAGGCCT CCCCTGGAAG GACCTTTACC AACAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa11317
    Clone ID Related Accession (Same CDS sequence) XM_015289991.2 , XM_015289991.1
    Accession Version XM_015289991.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3279bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product semaphorin-5B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGTTGTTT CAAGATTAAA GGCCATTTCC CTGTCATTGC CAAGCCTTTT TCTTCTTGCT 
    TTTCATCTCT CTGCATCACA GAATGTACCT GAGTACTCTG AAGCTGAACA TCAGCAGTGT
    GTCAGGAAAG AACACCCCAC AATCGCTTTT GAAGATCTGA AGCCTTGGGT GTCCAATTTC
    ACCTATCCAG GTGTGCATGA TTTTTCTCAG CTCGCCCTGG ATGCCAACAG GAACCAGCTC
    ATTGTGGGAG CAAGGAACTA CCTCTTCAGA CTTAGTCTAC ACAACGTTTC TCTTATTCAG
    GCAACAGCAT GGGGTTCAGA TGAAGACACC AGGAGGTCCT GTCAAAGTAA AGGGAAAACA
    GAGGAGGAGT GCCAGAACTA TGTCCGAGTG CTGATTGTTT ATGGCAAGAA GGTTTTTACC
    TGTGGTACCA ATGCCTTTTC ACCAGTGTGT TCCAGCCGTC AGGTAGGAAA TCTTAGCAAA
    ATCATTGACA GAATTAATGG AGTGGCTCGG TGCCCATATG ACCCCCGGCA TAACTCAACA
    GCTGTGATTA CATCACGAGG AGAACTCTAT GCTGCAACTG TAATTGATTT CTCTGGACGG
    GATCCTGCTA TTTACCGCAG CCTTGGAAAT GTGCCCCCAC TCAGGACAGC ACAGTACAAC
    TCCAAATGGC TCAATGAGCC TAACTTTATC GCTGCCTATG ACATCGGTTT GTTCACCTAC
    TTCTTCTTTC GAGAGAATGC GGTAGAACAC GATTGTGGGA AAACAGTATA TTCTCGTGTG
    GCCAGGGTCT GCAAAAACGA CATTGGAGGG AGATTTTTGC TGGAGGACAC GTGGACAACT
    TTCATGAAAG CCAGGCTGAA CTGCTCCAGA GCTGGAGAAA TCCCTTTCTA TTATAATGAA
    CTGCAAAGCA CCTTCTACCT TCCTGAACAA GACCTGATCT ATGGTGTCTT CACTACTAAT
    GTAAACAGCA TAGCTGCCTC AGCAGTGTGT GCCTTCAATC TCAGTGCCAT TACTCAGGCA
    TTTAATGGTC CTTTCCGTTA CCAAGAGAAC CCAAGATCAG CCTGGCTGCC AACAGCAAAC
    CCCATCCCAA ATTTCCAGTG TGGGACACTG AGCGATGACA GCCCGAATGA GAACCTGACC
    GAGAGAGTCC TACAGGACGC GCAGCGCTTG TTCCTGATGA ACGACGTGGT GCAGCCGGTC
    ACTGTAGATC CGTATGTGAC TCAGGACAGC ATTCGATTCT CTAAACTAGT GGTTGATATT
    GTTCAGGGGA AGGATACTCT CTACCACGTG ATGTACATTG GAACAGAGTA TGGCACCATC
    TTGAAGGCCC TTTCTACCAC TAATAGGAGC CTGAGGAGTT GTTATTTAGA GGAAATGCAG
    ATCCTCCCTG ATGGACAACG GGAAGCAATC AAGAGTCTCC AAATCCTGCA CAGCGACAGA
    TCGCTCTTTG TGGGCTTAAA TAACGGAGTG CTGAAAATTC CTCTGGAGAG ATGCTCCATG
    TACAGAACAG AAGGGGAATG CCTAGGAGCC AGAGACCCTT ACTGTGGCTG GGATAACAAG
    CAGAAGCGAT GCACCACCAT TGAGGACAGC TCCAACATGA GCCTATGGAC TCAAAATATT
    ACTGAGTGCC CAGTGAAAAA CCTGACAACA AATGGAAGAC TGGGGCCCTG GTCCCCATGG
    CAACCGTGTG AGCATTCAGA TGGGGACAGC ACAGGCTCCT GTATGTGTAG GTCACGTTCG
    TGTGACAGCC CTCGTCCTCG CTGTGGAGGT CGCAGCTGTG AAGGGGCCAG GATCGAGGTC
    GCAAATTGTT CAAGAAATGG CGCTTGGACA CCCTGGTCTT CCTGGGCCCT GTGCAGCACC
    TCCTGTGGGA TAGGCTTTCA GGTCCGCCAG CGGTCCTGCA GCAACCCTGC TCCACGGTAT
    GGGGGCAGGG TCTGTGTTGG ACAGAGCAGG GAAGAGAGAT TCTGTAATGA GAATAGTCCA
    TGTCCATTGC CAATTTTTTG GTCTTCGTGG GGTCCCTGGA ATAAATGTAG TGTGAATTGT
    GGTGGAGGAA TTCACTCAAG GCAAAGGACC TGTGAAAATG GAAATACATG TCCAGGCTGT
    GCTGTGGAAT ATAAGACCTG CAACCCAGAG AGCTGCCCAG AAGTGCGTAG GAACACACCC
    TGGACCCCTT GGATGCCTGT CAACATCACC CAGAATGGTG CCCGCCAGGA GCAGCGCTTC
    CGCTATACGT GTCGTGCCCA GCTGTCTGAT CCCCATGAGC TGCAGTTTGG GAGGAAGAAA
    ACTGAGAGTC GATTCTGTCC CAATGATGGC TCAGCTATGT GTGAAACTGA CTCTCTTGTT
    GACGACCTCC TCAAGACTGG TAAGACCTCT GCACGGATTA TCAATGGGGG CTGGTCCTTT
    TGGGGGGCCT GGTCTTCCTG TTCCAGGGAC TGTGAGTTGG GCTTTAGAAT CCGGAAAAGA
    ACATGCACAA ACCCTGAACC TAAAAATGGT GGCTTGCCTT GCGTGGGGTC AGCGATGGAG
    TACCAAGACT GCAACCCACA TCCTTGCCCA GTAAAAGGCT CATGGTCTTG CTGGACTCCT
    TGGTCTCAGT GCTCAGCAAC CTGTGGAGGA GGGCATTACC AGCGCACACG GACTTGCACC
    AACCCAGCTC CATCCAGTGG AGAGGATATC TGCATCGGGC TGCACACTGA GGAGGCTCTC
    TGCAACACCC ACCCATGTGA AGGTGGCTGG TCAGAGTGGT CAGAGTGGAG CCTGTGCAAT
    GAAGAGGGCA TCCAGTATCG CAGCCGTTAC TGTGAGGTAC ACAGCCCAGA CTCTTCTCAG
    TGCGTTGGAA ACAGCACACA ATACCAAGAT TGCCTGTATA ATGAAATTCC TGTGATCCTG
    CCAGCATCCA GCATTGATGA GAGCACAAAC TGTGGAGGTT TTAGTCTCAT CCATCTGATT
    GCCACTGGGG TCTCCTGCTT CTTTGGCTCT AGCCTCTTAA CGTTTGTGAT TTACGTGTAC
    TGCCAGCGCT GCCAGAGGCA GTCCCAGGAG TCGACCGTTA TCCACCCAAC CACTCCCAAC
    CATCTTCATT ACAAGGGCAA CACAACCCCC AAGAATGAGA AGTACACCCC CATGGAGTTC
    AAGACACTTA ACAAGAACAA CTTGATACCA GACGATAGGA CCAACTTCTA CCCCTTGCAG
    CAAACCAACG TGTACACGAC AACCTACTAT CCCAGTACAC TGAATAAATA TGACTACAGG
    CCCGAGGCCT CCCCTGGAAG GACCTTTACC AACAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145477.1
    CDS605..3883
    Translation

    Target ORF information:

    RefSeq Version XM_015289991.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (SEMA5B), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289991.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGTTGTTT CAAGATTAAA GGCCATTTCC CTGTCATTGC CAAGCCTTTT TCTTCTTGCT 
    TTTCATCTCT CTGCATCACA GAATGTACCT GAGTACTCTG AAGCTGAACA TCAGCAGTGT
    GTCAGGAAAG AACACCCCAC AATCGCTTTT GAAGATCTGA AGCCTTGGGT GTCCAATTTC
    ACCTATCCAG GTGTGCATGA TTTTTCTCAG CTCGCCCTGG ATGCCAACAG GAACCAGCTC
    ATTGTGGGAG CAAGGAACTA CCTCTTCAGA CTTAGTCTAC ACAACGTTTC TCTTATTCAG
    GCAACAGCAT GGGGTTCAGA TGAAGACACC AGGAGGTCCT GTCAAAGTAA AGGGAAAACA
    GAGGAGGAGT GCCAGAACTA TGTCCGAGTG CTGATTGTTT ATGGCAAGAA GGTTTTTACC
    TGTGGTACCA ATGCCTTTTC ACCAGTGTGT TCCAGCCGTC AGGTAGGAAA TCTTAGCAAA
    ATCATTGACA GAATTAATGG AGTGGCTCGG TGCCCATATG ACCCCCGGCA TAACTCAACA
    GCTGTGATTA CATCACGAGG AGAACTCTAT GCTGCAACTG TAATTGATTT CTCTGGACGG
    GATCCTGCTA TTTACCGCAG CCTTGGAAAT GTGCCCCCAC TCAGGACAGC ACAGTACAAC
    TCCAAATGGC TCAATGAGCC TAACTTTATC GCTGCCTATG ACATCGGTTT GTTCACCTAC
    TTCTTCTTTC GAGAGAATGC GGTAGAACAC GATTGTGGGA AAACAGTATA TTCTCGTGTG
    GCCAGGGTCT GCAAAAACGA CATTGGAGGG AGATTTTTGC TGGAGGACAC GTGGACAACT
    TTCATGAAAG CCAGGCTGAA CTGCTCCAGA GCTGGAGAAA TCCCTTTCTA TTATAATGAA
    CTGCAAAGCA CCTTCTACCT TCCTGAACAA GACCTGATCT ATGGTGTCTT CACTACTAAT
    GTAAACAGCA TAGCTGCCTC AGCAGTGTGT GCCTTCAATC TCAGTGCCAT TACTCAGGCA
    TTTAATGGTC CTTTCCGTTA CCAAGAGAAC CCAAGATCAG CCTGGCTGCC AACAGCAAAC
    CCCATCCCAA ATTTCCAGTG TGGGACACTG AGCGATGACA GCCCGAATGA GAACCTGACC
    GAGAGAGTCC TACAGGACGC GCAGCGCTTG TTCCTGATGA ACGACGTGGT GCAGCCGGTC
    ACTGTAGATC CGTATGTGAC TCAGGACAGC ATTCGATTCT CTAAACTAGT GGTTGATATT
    GTTCAGGGGA AGGATACTCT CTACCACGTG ATGTACATTG GAACAGAGTA TGGCACCATC
    TTGAAGGCCC TTTCTACCAC TAATAGGAGC CTGAGGAGTT GTTATTTAGA GGAAATGCAG
    ATCCTCCCTG ATGGACAACG GGAAGCAATC AAGAGTCTCC AAATCCTGCA CAGCGACAGA
    TCGCTCTTTG TGGGCTTAAA TAACGGAGTG CTGAAAATTC CTCTGGAGAG ATGCTCCATG
    TACAGAACAG AAGGGGAATG CCTAGGAGCC AGAGACCCTT ACTGTGGCTG GGATAACAAG
    CAGAAGCGAT GCACCACCAT TGAGGACAGC TCCAACATGA GCCTATGGAC TCAAAATATT
    ACTGAGTGCC CAGTGAAAAA CCTGACAACA AATGGAAGAC TGGGGCCCTG GTCCCCATGG
    CAACCGTGTG AGCATTCAGA TGGGGACAGC ACAGGCTCCT GTATGTGTAG GTCACGTTCG
    TGTGACAGCC CTCGTCCTCG CTGTGGAGGT CGCAGCTGTG AAGGGGCCAG GATCGAGGTC
    GCAAATTGTT CAAGAAATGG CGCTTGGACA CCCTGGTCTT CCTGGGCCCT GTGCAGCACC
    TCCTGTGGGA TAGGCTTTCA GGTCCGCCAG CGGTCCTGCA GCAACCCTGC TCCACGGTAT
    GGGGGCAGGG TCTGTGTTGG ACAGAGCAGG GAAGAGAGAT TCTGTAATGA GAATAGTCCA
    TGTCCATTGC CAATTTTTTG GTCTTCGTGG GGTCCCTGGA ATAAATGTAG TGTGAATTGT
    GGTGGAGGAA TTCACTCAAG GCAAAGGACC TGTGAAAATG GAAATACATG TCCAGGCTGT
    GCTGTGGAAT ATAAGACCTG CAACCCAGAG AGCTGCCCAG AAGTGCGTAG GAACACACCC
    TGGACCCCTT GGATGCCTGT CAACATCACC CAGAATGGTG CCCGCCAGGA GCAGCGCTTC
    CGCTATACGT GTCGTGCCCA GCTGTCTGAT CCCCATGAGC TGCAGTTTGG GAGGAAGAAA
    ACTGAGAGTC GATTCTGTCC CAATGATGGC TCAGCTATGT GTGAAACTGA CTCTCTTGTT
    GACGACCTCC TCAAGACTGG TAAGACCTCT GCACGGATTA TCAATGGGGG CTGGTCCTTT
    TGGGGGGCCT GGTCTTCCTG TTCCAGGGAC TGTGAGTTGG GCTTTAGAAT CCGGAAAAGA
    ACATGCACAA ACCCTGAACC TAAAAATGGT GGCTTGCCTT GCGTGGGGTC AGCGATGGAG
    TACCAAGACT GCAACCCACA TCCTTGCCCA GTAAAAGGCT CATGGTCTTG CTGGACTCCT
    TGGTCTCAGT GCTCAGCAAC CTGTGGAGGA GGGCATTACC AGCGCACACG GACTTGCACC
    AACCCAGCTC CATCCAGTGG AGAGGATATC TGCATCGGGC TGCACACTGA GGAGGCTCTC
    TGCAACACCC ACCCATGTGA AGGTGGCTGG TCAGAGTGGT CAGAGTGGAG CCTGTGCAAT
    GAAGAGGGCA TCCAGTATCG CAGCCGTTAC TGTGAGGTAC ACAGCCCAGA CTCTTCTCAG
    TGCGTTGGAA ACAGCACACA ATACCAAGAT TGCCTGTATA ATGAAATTCC TGTGATCCTG
    CCAGCATCCA GCATTGATGA GAGCACAAAC TGTGGAGGTT TTAGTCTCAT CCATCTGATT
    GCCACTGGGG TCTCCTGCTT CTTTGGCTCT AGCCTCTTAA CGTTTGTGAT TTACGTGTAC
    TGCCAGCGCT GCCAGAGGCA GTCCCAGGAG TCGACCGTTA TCCACCCAAC CACTCCCAAC
    CATCTTCATT ACAAGGGCAA CACAACCCCC AAGAATGAGA AGTACACCCC CATGGAGTTC
    AAGACACTTA ACAAGAACAA CTTGATACCA GACGATAGGA CCAACTTCTA CCCCTTGCAG
    CAAACCAACG TGTACACGAC AACCTACTAT CCCAGTACAC TGAATAAATA TGACTACAGG
    CCCGAGGCCT CCCCTGGAAG GACCTTTACC AACAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.