NEK1 cDNA ORF clone, Gallus gallus(chicken)

The following NEK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa150710 XM_025150496.1
Latest version!
Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OGa34987 XM_015285378.2
Latest version!
Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa34988 XM_015285380.2
Latest version!
Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa34989 XM_015285383.2
Latest version!
Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa07767 XM_015285375.2
Latest version!
Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa07767 XM_015285374.2
Latest version!
Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa07767 XM_420401.6
Latest version!
Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa07767 XM_015285376.2
Latest version!
Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa07768 XM_015285379.2
Latest version!
Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa07769 XM_015285381.2
Latest version!
Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa07770 XM_015285382.2
Latest version!
Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OGa07769 XM_015285381.1 Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa34987 XM_015285378.1 Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa07770 XM_015285382.1 Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OGa07767 XM_420401.5 Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa07767 XM_015285374.1 Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa07767 XM_015285376.1 Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa07767 XM_015285375.1 Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa07768 XM_015285379.1 Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa34988 XM_015285380.1 Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa34989 XM_015285383.1 Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa150710
    Clone ID Related Accession (Same CDS sequence) XM_025150496.1
    Accession Version XM_025150496.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3615bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase Nek1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
    GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG
    TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT
    CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG
    GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC
    TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT
    GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA
    ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTGATAT CTGGGCCCTT
    GGTTGCGTTC TCTATGAAAT GTGCACACTT AAGCATGCAT TTGAAGCTGG TAACATGAAG
    AACTTGGTAC TGAAGATAAT CTCTGGATCT TTCCCTCCTG TTTCTGTGCA TTATTCCTAT
    GACCTACGTA ATCTACTGTC ACAGTTATTT AAAAGGAATC CTAGGGATAG ACCATCAGTG
    AACTCCATAT TGGAGAAAAA TTTCATAGCC AAACGGGTTG AAAAATTTCT CACACCGCAG
    CTTATTGCAG AAGAATTCAG TCACAAAGTG TTTCACAAGT TTGGACCACA TGCTGGACCA
    GCTAAAAGAC CAGCTCAAGG ACATATCTCG GCTTCAATTG CACCAGTGCA GAAAATTACC
    AAACCTGCTG CAAAATACGG AGTGCCTTTA GCGATGAAGA AGTGTTATGA CGCACCCAAA
    AAGCTTCATG AGAAAAAGTC ATTGATTAAA CTTAGGCAGG CCTTTCCAAC TCCAAAGAAG
    AAAATTATTC CAGGAGAAGA AAGGAGGAAA ATGTTTGAGG AACCTTCAAA AAAGAAAAGG
    TTAGAATTGC CTGAAAAAGA AAAACGCCAG AGAGATCAGA TCAGTTTACT GAAGGCAGAG
    GAAATGAGAA GATTGGAAAA AGAAAGGATG GAACGAATAA ACAGAGCCAG GGAGCAAGGA
    TGGAGGAATG TGCTTGGTTC AGGTGGAAGT GGTGATGTTA AGGCACCATA TTATGGCGGT
    GGAGGTGGTG TTGGTCCTTT TCCTGCCTCT TTCAGAGGAC AGTATGAGCA CTATCATGCT
    ATTTTTGACC AGATGCAAAA GGAAACCTTC AAAGTAACTA AAGAGAGGCA AGCCAAATGG
    AGGGCGGAGG TACAAGGCCA AGAAGCTGTT GAGAGAGGAA TTCCACCTGG AGTACGTCCA
    GGAGTTCCTA AGGGGCCTGC AGGTCATCAC CATTTACCTG ATGCTGATGC AATTAGGAAA
    AAAGTGAAAA GGTTGAAGGA GGTGTCTAAA CAAGCCAGTG CAAACAGGCA GAAAGGATGG
    CTACCTGCAG AAAGAGCAAA GCAAGTTGAA GAATTCTGGC AACGAAAGAG GGAAGCCATG
    CAAAACAAAG CTCGTGCTGA GGGACACATG GGTACACTGC AAAACGTGGC AGATATCTAT
    GGAGGCAGGC CCATGTCTGC AAGAGGAAGG AAATCCAGAA ACAAGGAAGA AGAGGAATAT
    TTGGCAAGAT TAAGACAGAT ACGACTACAA AACTTCAATG AGCGTCAACA GATTAAAGCA
    AAACTTCGTG GAGAGAAGAA TGAAGCTCCC AGTTCTGATG GACAAGAGTC AAGTGAGGAA
    GCAGAAATGA AACGCAAAAA GATAGAAGCA CAGAAGGCCC AAGCAAATGC TCGAGCTGCA
    GTTCTGAAAG AACAGTTAGA GCGAAAGAGG AAGGAGGCAT ATGAAAGAGA GAAAAGAGCT
    TGGGAAGAAC ATCTGATAGC AAAAGGAGTA AAGAATCCTA ATGTGCCTTT TCATGTGGGA
    GCAGCAGGAC ACACTCCTGT GCATGGAATA CAAGAGCCTG GAGTGTCTCC TCCTAAGCAA
    CAGCTTCCAG TTAAGCCCAT CTCCATGACT TCTGCTTTGA AAGAAGTTGG CGTGGATGGA
    AATGTAACCG CTGCCCAGGA GGTTAAAGAG GAAATAAAAA AGCCAGATCT TACAATGCAG
    AACAAGAGGC AGATACTGAA AAGATTGAAT CAAAATCTTA AAGCTCAGGA AGATGAGAAA
    GGAAGAAAGG ACGATAAAAA TATCTCTGAG TCAGATGGGT CTGAAGATGG TAAAGAAGAG
    CAAGAAGATA ACCATCCACT TCTGGGAGAT CGCAAAAGAT GGGCATCAAG GGCATCAGAT
    TTGGTAGTTC CTTTAGCTCA GTTATCAATG GAAGATTCTC TCTCTGGAAC AGACCGGCAA
    ACTATGGGGG AAGTAATCAG GCTAGATATT GGGGAACCCC GCAGGAAAGT CTGGGGCAAA
    AGCCCTACTG ATTCGGTCCT GAAGATTCTA GGAGAAGCGG AGTTGCAGCT ACCAACTGAA
    TTACTGGAAG AAATAGATGT AATAAATGAG CCTGATGATT TGGAAGCAGT GATGTTGAAA
    GAAACAGAAG AAGATAAAAA GCACCAGAGC AAGGAATTTC CCATTGCTGT TAATGAAGTG
    TGGGTGAAAG AAAAACAGGA TGAGGCACAA CAGGATGCAA GAAATGAAGC AGCTTTTGAG
    AAAGAAGTAG TGCTTGATGA AGTGCAATCA CAAAATGAAG CTCCAGAAGA AACTTGTTCC
    AGTGACTCTC TGCAACCTGA ACCGTTATTC CAGAGAGTAA TACAGCCCCC AGCTGTACCT
    ACAGCCTCAC CAGTTCTATC ATCTCAGTCT TCCCAAGAAG AGCCTTTTGT ACCTCGTTCA
    CGTTCCATAT CGCCAGCAAA AAATAAAGGG AAAAGTTCAT TGTTTTTTGG ACTTTCTACA
    GGTCTGTTTG ATGCAAACAA CCCAAAGATG TTGAGAACGT GTTCACTCCC GGATCTTTAC
    AAACTGTACA GAACCTTAGT TGATGTTCCC AGCATAGCAG ATGTACAGCA TCAACACAGT
    CTTGAAATAG ATGATACGGA AGATGAGCAA GCCAAAGAAG GACCCTCTGA TTCTGAGGAT
    ATCATGTTTG GGGACGCCGA TACAGATTTG CAGGAACTCC GGGCCTCAAT GGAACAATTA
    CTTAGGGAGC AGCCTAGTGA GGAATTAAGT GAAGAGGAGG AATCTACTTT AAAGGCTAAC
    GTCATCAAAT GCATGACAAA TGGTACAGAG CTGGATGAAG CAGATGAAAA TAACCCCAGC
    AGTGAAAGTG CACTTAATGA AGAATGGCAG TCAGATAATA GTGATGGAGA TATTGCCAGT
    GAATGTGAAG AATGTGATAG TGTCTTCAGC CATTTGGAAG AGCTGAGATA CAACCTGGAA
    CAGGAAATAG GCTTTGAGAA ATTAATTGAA GTTTATAATA AAATAAAGGC CATACATGAA
    GATGAAGATG AAAATATTGA TATTTGTTCA AGCATAGTTC AGACTATTCT GGGAAATGAA
    CACAAACACT TATATGCCAA AGTACTTCAC CTGGTCATGG CGGATGGAGC CTACCAGGAA
    GATAATGACG AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025006264.1
    CDS1005..4619
    Translation

    Target ORF information:

    RefSeq Version XM_025150496.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025150496.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
    GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG
    TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT
    CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG
    GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC
    TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT
    GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA
    ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTGATAT CTGGGCCCTT
    GGTTGCGTTC TCTATGAAAT GTGCACACTT AAGCATGCAT TTGAAGCTGG TAACATGAAG
    AACTTGGTAC TGAAGATAAT CTCTGGATCT TTCCCTCCTG TTTCTGTGCA TTATTCCTAT
    GACCTACGTA ATCTACTGTC ACAGTTATTT AAAAGGAATC CTAGGGATAG ACCATCAGTG
    AACTCCATAT TGGAGAAAAA TTTCATAGCC AAACGGGTTG AAAAATTTCT CACACCGCAG
    CTTATTGCAG AAGAATTCAG TCACAAAGTG TTTCACAAGT TTGGACCACA TGCTGGACCA
    GCTAAAAGAC CAGCTCAAGG ACATATCTCG GCTTCAATTG CACCAGTGCA GAAAATTACC
    AAACCTGCTG CAAAATACGG AGTGCCTTTA GCGATGAAGA AGTGTTATGA CGCACCCAAA
    AAGCTTCATG AGAAAAAGTC ATTGATTAAA CTTAGGCAGG CCTTTCCAAC TCCAAAGAAG
    AAAATTATTC CAGGAGAAGA AAGGAGGAAA ATGTTTGAGG AACCTTCAAA AAAGAAAAGG
    TTAGAATTGC CTGAAAAAGA AAAACGCCAG AGAGATCAGA TCAGTTTACT GAAGGCAGAG
    GAAATGAGAA GATTGGAAAA AGAAAGGATG GAACGAATAA ACAGAGCCAG GGAGCAAGGA
    TGGAGGAATG TGCTTGGTTC AGGTGGAAGT GGTGATGTTA AGGCACCATA TTATGGCGGT
    GGAGGTGGTG TTGGTCCTTT TCCTGCCTCT TTCAGAGGAC AGTATGAGCA CTATCATGCT
    ATTTTTGACC AGATGCAAAA GGAAACCTTC AAAGTAACTA AAGAGAGGCA AGCCAAATGG
    AGGGCGGAGG TACAAGGCCA AGAAGCTGTT GAGAGAGGAA TTCCACCTGG AGTACGTCCA
    GGAGTTCCTA AGGGGCCTGC AGGTCATCAC CATTTACCTG ATGCTGATGC AATTAGGAAA
    AAAGTGAAAA GGTTGAAGGA GGTGTCTAAA CAAGCCAGTG CAAACAGGCA GAAAGGATGG
    CTACCTGCAG AAAGAGCAAA GCAAGTTGAA GAATTCTGGC AACGAAAGAG GGAAGCCATG
    CAAAACAAAG CTCGTGCTGA GGGACACATG GGTACACTGC AAAACGTGGC AGATATCTAT
    GGAGGCAGGC CCATGTCTGC AAGAGGAAGG AAATCCAGAA ACAAGGAAGA AGAGGAATAT
    TTGGCAAGAT TAAGACAGAT ACGACTACAA AACTTCAATG AGCGTCAACA GATTAAAGCA
    AAACTTCGTG GAGAGAAGAA TGAAGCTCCC AGTTCTGATG GACAAGAGTC AAGTGAGGAA
    GCAGAAATGA AACGCAAAAA GATAGAAGCA CAGAAGGCCC AAGCAAATGC TCGAGCTGCA
    GTTCTGAAAG AACAGTTAGA GCGAAAGAGG AAGGAGGCAT ATGAAAGAGA GAAAAGAGCT
    TGGGAAGAAC ATCTGATAGC AAAAGGAGTA AAGAATCCTA ATGTGCCTTT TCATGTGGGA
    GCAGCAGGAC ACACTCCTGT GCATGGAATA CAAGAGCCTG GAGTGTCTCC TCCTAAGCAA
    CAGCTTCCAG TTAAGCCCAT CTCCATGACT TCTGCTTTGA AAGAAGTTGG CGTGGATGGA
    AATGTAACCG CTGCCCAGGA GGTTAAAGAG GAAATAAAAA AGCCAGATCT TACAATGCAG
    AACAAGAGGC AGATACTGAA AAGATTGAAT CAAAATCTTA AAGCTCAGGA AGATGAGAAA
    GGAAGAAAGG ACGATAAAAA TATCTCTGAG TCAGATGGGT CTGAAGATGG TAAAGAAGAG
    CAAGAAGATA ACCATCCACT TCTGGGAGAT CGCAAAAGAT GGGCATCAAG GGCATCAGAT
    TTGGTAGTTC CTTTAGCTCA GTTATCAATG GAAGATTCTC TCTCTGGAAC AGACCGGCAA
    ACTATGGGGG AAGTAATCAG GCTAGATATT GGGGAACCCC GCAGGAAAGT CTGGGGCAAA
    AGCCCTACTG ATTCGGTCCT GAAGATTCTA GGAGAAGCGG AGTTGCAGCT ACCAACTGAA
    TTACTGGAAG AAATAGATGT AATAAATGAG CCTGATGATT TGGAAGCAGT GATGTTGAAA
    GAAACAGAAG AAGATAAAAA GCACCAGAGC AAGGAATTTC CCATTGCTGT TAATGAAGTG
    TGGGTGAAAG AAAAACAGGA TGAGGCACAA CAGGATGCAA GAAATGAAGC AGCTTTTGAG
    AAAGAAGTAG TGCTTGATGA AGTGCAATCA CAAAATGAAG CTCCAGAAGA AACTTGTTCC
    AGTGACTCTC TGCAACCTGA ACCGTTATTC CAGAGAGTAA TACAGCCCCC AGCTGTACCT
    ACAGCCTCAC CAGTTCTATC ATCTCAGTCT TCCCAAGAAG AGCCTTTTGT ACCTCGTTCA
    CGTTCCATAT CGCCAGCAAA AAATAAAGGG AAAAGTTCAT TGTTTTTTGG ACTTTCTACA
    GGTCTGTTTG ATGCAAACAA CCCAAAGATG TTGAGAACGT GTTCACTCCC GGATCTTTAC
    AAACTGTACA GAACCTTAGT TGATGTTCCC AGCATAGCAG ATGTACAGCA TCAACACAGT
    CTTGAAATAG ATGATACGGA AGATGAGCAA GCCAAAGAAG GACCCTCTGA TTCTGAGGAT
    ATCATGTTTG GGGACGCCGA TACAGATTTG CAGGAACTCC GGGCCTCAAT GGAACAATTA
    CTTAGGGAGC AGCCTAGTGA GGAATTAAGT GAAGAGGAGG AATCTACTTT AAAGGCTAAC
    GTCATCAAAT GCATGACAAA TGGTACAGAG CTGGATGAAG CAGATGAAAA TAACCCCAGC
    AGTGAAAGTG CACTTAATGA AGAATGGCAG TCAGATAATA GTGATGGAGA TATTGCCAGT
    GAATGTGAAG AATGTGATAG TGTCTTCAGC CATTTGGAAG AGCTGAGATA CAACCTGGAA
    CAGGAAATAG GCTTTGAGAA ATTAATTGAA GTTTATAATA AAATAAAGGC CATACATGAA
    GATGAAGATG AAAATATTGA TATTTGTTCA AGCATAGTTC AGACTATTCT GGGAAATGAA
    CACAAACACT TATATGCCAA AGTACTTCAC CTGGTCATGG CGGATGGAGC CTACCAGGAA
    GATAATGACG AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34987
    Clone ID Related Accession (Same CDS sequence) XM_015285378.1 , XM_015285378.2
    Accession Version XM_015285378.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3642bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase Nek1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455855.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
    GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG
    TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT
    CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG
    GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC
    TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT
    GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA
    ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT
    CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC
    AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG
    CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC
    CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA
    AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA
    CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT
    CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT
    TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG
    ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT
    AGGCAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA
    GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA
    CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT
    GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC
    AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA
    GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG
    AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT
    TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA
    GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA
    TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT
    ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA
    TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC
    TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT
    TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG
    AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG
    GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG
    AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA
    GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT
    GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA
    ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA
    AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA
    GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC
    AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA
    GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG
    GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA
    GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT
    GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG
    GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG
    GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA
    AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG
    AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC
    CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA
    AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG
    AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC
    ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC
    AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG
    GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA
    GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG
    GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA
    GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT
    TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT
    TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC
    ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG
    GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140864.1
    CDS1007..4648
    Translation

    Target ORF information:

    RefSeq Version XM_015285378.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285378.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
    GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG
    TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT
    CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG
    GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC
    TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT
    GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA
    ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT
    CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC
    AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG
    CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC
    CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA
    AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA
    CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT
    CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT
    TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG
    ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT
    AGGCAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA
    GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA
    CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT
    GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC
    AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA
    GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG
    AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT
    TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA
    GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA
    TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT
    ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA
    TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC
    TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT
    TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG
    AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG
    GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG
    AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA
    GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT
    GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA
    ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA
    AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA
    GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC
    AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA
    GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG
    GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA
    GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT
    GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG
    GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG
    GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA
    AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG
    AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC
    CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA
    AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG
    AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC
    ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC
    AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG
    GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA
    GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG
    GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA
    GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT
    TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT
    TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC
    ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG
    GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34987
    Clone ID Related Accession (Same CDS sequence) XM_015285378.1 , XM_015285378.2
    Accession Version XM_015285378.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3642bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase Nek1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285378.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
    GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG
    TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT
    CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG
    GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC
    TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT
    GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA
    ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT
    CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC
    AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG
    CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC
    CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA
    AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA
    CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT
    CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT
    TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG
    ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT
    AGGCAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA
    GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA
    CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT
    GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC
    AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA
    GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG
    AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT
    TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA
    GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA
    TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT
    ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA
    TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC
    TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT
    TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG
    AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG
    GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG
    AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA
    GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT
    GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA
    ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA
    AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA
    GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC
    AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA
    GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG
    GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA
    GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT
    GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG
    GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG
    GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA
    AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG
    AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC
    CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA
    AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG
    AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC
    ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC
    AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG
    GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA
    GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG
    GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA
    GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT
    TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT
    TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC
    ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG
    GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140864.1
    CDS1004..4645
    Translation

    Target ORF information:

    RefSeq Version XM_015285378.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285378.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
    GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG
    TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT
    CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG
    GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC
    TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT
    GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA
    ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT
    CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC
    AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG
    CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC
    CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA
    AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA
    CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT
    CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT
    TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG
    ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT
    AGGCAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA
    GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA
    CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT
    GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC
    AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA
    GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG
    AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT
    TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA
    GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA
    TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT
    ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA
    TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC
    TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT
    TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG
    AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG
    GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG
    AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA
    GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT
    GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA
    ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA
    AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA
    GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC
    AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA
    GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG
    GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA
    GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT
    GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG
    GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG
    GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA
    AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG
    AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC
    CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA
    AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG
    AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC
    ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC
    AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG
    GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA
    GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG
    GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA
    GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT
    TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT
    TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC
    ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG
    GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34988
    Clone ID Related Accession (Same CDS sequence) XM_015285380.1 , XM_015285380.2
    Accession Version XM_015285380.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3582bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase Nek1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455855.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
    GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG
    TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT
    CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG
    GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC
    TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT
    GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA
    ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT
    CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC
    AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG
    CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC
    CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA
    AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA
    CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT
    CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT
    TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG
    ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT
    AGGCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA
    CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT
    GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC
    AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA
    GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG
    AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT
    TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA
    GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA
    TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT
    ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA
    TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC
    TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT
    TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG
    AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG
    GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG
    AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA
    GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT
    GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA
    ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA
    AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA
    GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC
    AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA
    GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG
    GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA
    GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT
    GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG
    GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG
    GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA
    AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG
    AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC
    CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA
    AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG
    AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC
    ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC
    AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG
    GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA
    GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG
    GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA
    GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT
    TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT
    TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC
    ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG
    GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140866.1
    CDS1006..4587
    Translation

    Target ORF information:

    RefSeq Version XM_015285380.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285380.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
    GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG
    TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT
    CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG
    GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC
    TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT
    GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA
    ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT
    CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC
    AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG
    CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC
    CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA
    AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA
    CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT
    CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT
    TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG
    ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT
    AGGCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA
    CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT
    GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC
    AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA
    GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG
    AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT
    TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA
    GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA
    TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT
    ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA
    TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC
    TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT
    TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG
    AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG
    GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG
    AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA
    GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT
    GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA
    ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA
    AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA
    GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC
    AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA
    GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG
    GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA
    GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT
    GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG
    GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG
    GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA
    AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG
    AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC
    CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA
    AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG
    AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC
    ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC
    AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG
    GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA
    GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG
    GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA
    GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT
    TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT
    TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC
    ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG
    GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34988
    Clone ID Related Accession (Same CDS sequence) XM_015285380.1 , XM_015285380.2
    Accession Version XM_015285380.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3582bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase Nek1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285380.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
    GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG
    TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT
    CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG
    GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC
    TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT
    GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA
    ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT
    CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC
    AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG
    CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC
    CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA
    AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA
    CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT
    CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT
    TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG
    ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT
    AGGCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA
    CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT
    GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC
    AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA
    GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG
    AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT
    TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA
    GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA
    TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT
    ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA
    TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC
    TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT
    TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG
    AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG
    GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG
    AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA
    GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT
    GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA
    ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA
    AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA
    GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC
    AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA
    GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG
    GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA
    GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT
    GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG
    GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG
    GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA
    AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG
    AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC
    CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA
    AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG
    AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC
    ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC
    AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG
    GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA
    GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG
    GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA
    GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT
    TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT
    TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC
    ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG
    GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140866.1
    CDS1004..4585
    Translation

    Target ORF information:

    RefSeq Version XM_015285380.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285380.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
    GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG
    TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT
    CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG
    GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC
    TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT
    GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA
    ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT
    CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC
    AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG
    CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC
    CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA
    AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA
    CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT
    CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT
    TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG
    ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT
    AGGCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA
    CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT
    GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC
    AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA
    GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG
    AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT
    TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA
    GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA
    TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT
    ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA
    TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC
    TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT
    TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG
    AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG
    GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG
    AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA
    GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT
    GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA
    ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA
    AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA
    GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC
    AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA
    GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG
    GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA
    GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT
    GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG
    GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG
    GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA
    AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG
    AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC
    CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA
    AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG
    AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC
    ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC
    AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG
    GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA
    GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG
    GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA
    GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT
    TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT
    TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC
    ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG
    GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34989
    Clone ID Related Accession (Same CDS sequence) XM_015285383.1 , XM_015285383.2
    Accession Version XM_015285383.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3117bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase Nek1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455855.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTGCACAC TTAAGCATGC ATTTGAAGCT GGTAACATGA AGAACTTGGT ACTGAAGATA 
    ATCTCTGGAT CTTTCCCTCC TGTTTCTGTG CATTATTCCT ATGACCTACG TAATCTACTG
    TCACAGTTAT TTAAAAGGAA TCCTAGGGAT AGACCATCAG TGAACTCCAT ATTGGAGAAA
    AATTTCATAG CCAAACGGGT TGAAAAATTT CTCACACCGC AGCTTATTGC AGAAGAATTC
    AGTCACAAAG TGTTTCACAA GTTTGGACCA CATGCTGGAC CAGCTAAAAG ACCAGCTCAA
    GGACATATCT CGGCTTCAAT TGCACCAGTG CAGAAAATTA CCAAACCTGC TGCAAAATAC
    GGAGTGCCTT TAGCGATGAA GAAGTGTTAT GACGCACCCA AAAAGCTTCA TGAGAAAAAG
    TCATTGATTA AACTTAGGCA GGCCTTTCCA ACTCCAAAGA AGAAAATTAT TCCAGGAGAA
    GAAAGGAGGA AAATGTTTGA GGAACCTTCA AAAAAGAAAA GGTTAGAATT GCCTGAAAAA
    GAAAAACGCC AGAGAGATCA GATCAGTTTA CTGAAGGCAG AGGAAATGAG AAGATTGGAA
    AAAGAAAGGA TGGAACGAAT AAACAGAGCC AGGGAGCAAG GATGGAGGAA TGTGCTTGGT
    TCAGGTGGAA GTGGTGATGT TAAGGCACCA TATTATGGCG GTGGAGGTGG TGTTGGTCCT
    TTTCCTGCCT CTTTCAGAGG ACAGTATGAG CACTATCATG CTATTTTTGA CCAGATGCAA
    AAGGAAACCT TCAAAGTAAC TAAAGAGAGG CAAGCCAAAT GGAGGGCGGA GGTACAAGGC
    CAAGAAGCTG TTGAGAGAGG AATTCCACCT GGAGTACGTC CAGGAGTTCC TAAGGGGCCT
    GCAGGTCATC ACCATTTACC TGATGCTGAT GCAATTAGGA AAAAAGTGAA AAGGTTGAAG
    GAGGTGTCTA AACAAGCCAG TGCAAACAGG CAGAAAGGAT GGCTACCTGC AGAAAGAGCA
    AAGCAAGTTG AAGAATTCTG GCAACGAAAG AGGGAAGCCA TGCAAAACAA AGCTCGTGCT
    GAGGGACACA TGGGTACACT GCAAAACGTG GCAGATATCT ATGGAGGCAG GCCCATGTCT
    GCAAGAGGAA GGAAATCCAG AAACAAGGAA GAAGAGGAAT ATTTGGCAAG ATTAAGACAG
    ATACGACTAC AAAACTTCAA TGAGCGTCAA CAGATTAAAG CAAAACTTCG TGGAGAGAAG
    AATGAAGCTC CCAGTTCTGA TGGACAAGAG TCAAGTGAGG AAGCAGAAAT GAAACGCAAA
    AAGATAGAAG CACAGAAGGC CCAAGCAAAT GCTCGAGCTG CAGTTCTGAA AGAACAGTTA
    GAGCGAAAGA GGAAGGAGGC ATATGAAAGA GAGAAAAGAG CTTGGGAAGA ACATCTGATA
    GCAAAAGGAG TAAAGAATCC TAATGTGCCT TTTCATGTGG GAGCAGCAGG ACACACTCCT
    GTGCATGGAA TACAAGAGCC TGGAGTGTCT CCTCCTAAGC AACAGCTTCC AGTTAAGCCC
    ATCTCCATGA CTTCTGCTTT GAAAGAAGTT GGCGTGGATG GAAATGTAAC CGCTGCCCAG
    GAGGTTAAAG AGGAAATAAA AAAGCCAGAT CTTACAATGC AGAACAAGAG GCAGATACTG
    AAAAGATTGA ATCAAAATCT TAAAGCTCAG GAAGATGAGA AAGGAAGAAA GGACGATAAA
    AATATCTCTG AGTCAGATGG GTCTGAAGAT GGTAAAGAAG AGCAAGAAGA TAACCATCCA
    CTTCTGGGAG ATCGCAAAAG ATGGGCATCA AGGGCATCAG ATTTGGTAGT TCCTTTAGCT
    CAGTTATCAA TGGAAGATTC TCTCTCTGGA ACAGACCGGC AAACTATGGG GGAAGTAATC
    AGGCTAGATA TTGGGGAACC CCGCAGGAAA GTCTGGGGCA AAAGCCCTAC TGATTCGGTC
    CTGAAGATTC TAGGAGAAGC GGAGTTGCAG CTACCAACTG AATTACTGGA AGAAATAGAT
    GTAATAAATG AGCCTGATGA TTTGGAAGCA GTGATGTTGA AAGAAACAGA AGAAGATAAA
    AAGCACCAGA GCAAGGAATT TCCCATTGCT GTTAATGAAG TGTGGGTGAA AGAAAAACAG
    GATGAGGCAC AACAGGATGC AAGAAATGAA GCAGCTTTTG AGAAAGAAGT AGTGCTTGAT
    GAAGTGCAAT CACAAAATGA AGCTCCAGAA GAAACTTGTT CCAGTGACTC TCTGCAACCT
    GAACCGTTAT TCCAGAGAGT AATACAGCCC CCAGCTGTAC CTACAGCCTC ACCAGTTCTA
    TCATCTCAGT CTTCCCAAGA AGAGCCTTTT GTACCTCGTT CACGTTCCAT ATCGCCAGCA
    AAAAATAAAG GGAAAAGTTC ATTGTTTTTT GGACTTTCTA CAGGTCTGTT TGATGCAAAC
    AACCCAAAGA TGTTGAGAAC GTGTTCACTC CCGGATCTTT ACAAACTGTA CAGAACCTTA
    GTTGATGTTC CCAGCATAGC AGATGTACAG CATCAACACA GTCTTGAAAT AGATGATACG
    GAAGATGAGC AAGCCAAAGA AGGACCCTCT GATTCTGAGG ATATCATGTT TGGGGACGCC
    GATACAGATT TGCAGGAACT CCGGGCCTCA ATGGAACAAT TACTTAGGGA GCAGCCTAGT
    GAGGAATTAA GTGAAGAGGA GGAATCTACT TTAAAGGCTA ACGTCATCAA ATGCATGACA
    AATGGTACAG AGCTGGATGA AGCAGATGAA AATAACCCCA GCAGTGAAAG TGCACTTAAT
    GAAGAATGGC AGTCAGATAA TAGTGATGGA GATATTGCCA GTGAATGTGA AGAATGTGAT
    AGTGTCTTCA GCCATTTGGA AGAGCTGAGA TACAACCTGG AACAGGAAAT AGGCTTTGAG
    AAATTAATTG AAGTTTATAA TAAAATAAAG GCCATACATG AAGATGAAGA TGAAAATATT
    GATATTTGTT CAAGCATAGT TCAGACTATT CTGGGAAATG AACACAAACA CTTATATGCC
    AAAGTACTTC ACCTGGTCAT GGCGGATGGA GCCTACCAGG AAGATAATGA CGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140869.1
    CDS106..3222
    Translation

    Target ORF information:

    RefSeq Version XM_015285383.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285383.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTGCACAC TTAAGCATGC ATTTGAAGCT GGTAACATGA AGAACTTGGT ACTGAAGATA 
    ATCTCTGGAT CTTTCCCTCC TGTTTCTGTG CATTATTCCT ATGACCTACG TAATCTACTG
    TCACAGTTAT TTAAAAGGAA TCCTAGGGAT AGACCATCAG TGAACTCCAT ATTGGAGAAA
    AATTTCATAG CCAAACGGGT TGAAAAATTT CTCACACCGC AGCTTATTGC AGAAGAATTC
    AGTCACAAAG TGTTTCACAA GTTTGGACCA CATGCTGGAC CAGCTAAAAG ACCAGCTCAA
    GGACATATCT CGGCTTCAAT TGCACCAGTG CAGAAAATTA CCAAACCTGC TGCAAAATAC
    GGAGTGCCTT TAGCGATGAA GAAGTGTTAT GACGCACCCA AAAAGCTTCA TGAGAAAAAG
    TCATTGATTA AACTTAGGCA GGCCTTTCCA ACTCCAAAGA AGAAAATTAT TCCAGGAGAA
    GAAAGGAGGA AAATGTTTGA GGAACCTTCA AAAAAGAAAA GGTTAGAATT GCCTGAAAAA
    GAAAAACGCC AGAGAGATCA GATCAGTTTA CTGAAGGCAG AGGAAATGAG AAGATTGGAA
    AAAGAAAGGA TGGAACGAAT AAACAGAGCC AGGGAGCAAG GATGGAGGAA TGTGCTTGGT
    TCAGGTGGAA GTGGTGATGT TAAGGCACCA TATTATGGCG GTGGAGGTGG TGTTGGTCCT
    TTTCCTGCCT CTTTCAGAGG ACAGTATGAG CACTATCATG CTATTTTTGA CCAGATGCAA
    AAGGAAACCT TCAAAGTAAC TAAAGAGAGG CAAGCCAAAT GGAGGGCGGA GGTACAAGGC
    CAAGAAGCTG TTGAGAGAGG AATTCCACCT GGAGTACGTC CAGGAGTTCC TAAGGGGCCT
    GCAGGTCATC ACCATTTACC TGATGCTGAT GCAATTAGGA AAAAAGTGAA AAGGTTGAAG
    GAGGTGTCTA AACAAGCCAG TGCAAACAGG CAGAAAGGAT GGCTACCTGC AGAAAGAGCA
    AAGCAAGTTG AAGAATTCTG GCAACGAAAG AGGGAAGCCA TGCAAAACAA AGCTCGTGCT
    GAGGGACACA TGGGTACACT GCAAAACGTG GCAGATATCT ATGGAGGCAG GCCCATGTCT
    GCAAGAGGAA GGAAATCCAG AAACAAGGAA GAAGAGGAAT ATTTGGCAAG ATTAAGACAG
    ATACGACTAC AAAACTTCAA TGAGCGTCAA CAGATTAAAG CAAAACTTCG TGGAGAGAAG
    AATGAAGCTC CCAGTTCTGA TGGACAAGAG TCAAGTGAGG AAGCAGAAAT GAAACGCAAA
    AAGATAGAAG CACAGAAGGC CCAAGCAAAT GCTCGAGCTG CAGTTCTGAA AGAACAGTTA
    GAGCGAAAGA GGAAGGAGGC ATATGAAAGA GAGAAAAGAG CTTGGGAAGA ACATCTGATA
    GCAAAAGGAG TAAAGAATCC TAATGTGCCT TTTCATGTGG GAGCAGCAGG ACACACTCCT
    GTGCATGGAA TACAAGAGCC TGGAGTGTCT CCTCCTAAGC AACAGCTTCC AGTTAAGCCC
    ATCTCCATGA CTTCTGCTTT GAAAGAAGTT GGCGTGGATG GAAATGTAAC CGCTGCCCAG
    GAGGTTAAAG AGGAAATAAA AAAGCCAGAT CTTACAATGC AGAACAAGAG GCAGATACTG
    AAAAGATTGA ATCAAAATCT TAAAGCTCAG GAAGATGAGA AAGGAAGAAA GGACGATAAA
    AATATCTCTG AGTCAGATGG GTCTGAAGAT GGTAAAGAAG AGCAAGAAGA TAACCATCCA
    CTTCTGGGAG ATCGCAAAAG ATGGGCATCA AGGGCATCAG ATTTGGTAGT TCCTTTAGCT
    CAGTTATCAA TGGAAGATTC TCTCTCTGGA ACAGACCGGC AAACTATGGG GGAAGTAATC
    AGGCTAGATA TTGGGGAACC CCGCAGGAAA GTCTGGGGCA AAAGCCCTAC TGATTCGGTC
    CTGAAGATTC TAGGAGAAGC GGAGTTGCAG CTACCAACTG AATTACTGGA AGAAATAGAT
    GTAATAAATG AGCCTGATGA TTTGGAAGCA GTGATGTTGA AAGAAACAGA AGAAGATAAA
    AAGCACCAGA GCAAGGAATT TCCCATTGCT GTTAATGAAG TGTGGGTGAA AGAAAAACAG
    GATGAGGCAC AACAGGATGC AAGAAATGAA GCAGCTTTTG AGAAAGAAGT AGTGCTTGAT
    GAAGTGCAAT CACAAAATGA AGCTCCAGAA GAAACTTGTT CCAGTGACTC TCTGCAACCT
    GAACCGTTAT TCCAGAGAGT AATACAGCCC CCAGCTGTAC CTACAGCCTC ACCAGTTCTA
    TCATCTCAGT CTTCCCAAGA AGAGCCTTTT GTACCTCGTT CACGTTCCAT ATCGCCAGCA
    AAAAATAAAG GGAAAAGTTC ATTGTTTTTT GGACTTTCTA CAGGTCTGTT TGATGCAAAC
    AACCCAAAGA TGTTGAGAAC GTGTTCACTC CCGGATCTTT ACAAACTGTA CAGAACCTTA
    GTTGATGTTC CCAGCATAGC AGATGTACAG CATCAACACA GTCTTGAAAT AGATGATACG
    GAAGATGAGC AAGCCAAAGA AGGACCCTCT GATTCTGAGG ATATCATGTT TGGGGACGCC
    GATACAGATT TGCAGGAACT CCGGGCCTCA ATGGAACAAT TACTTAGGGA GCAGCCTAGT
    GAGGAATTAA GTGAAGAGGA GGAATCTACT TTAAAGGCTA ACGTCATCAA ATGCATGACA
    AATGGTACAG AGCTGGATGA AGCAGATGAA AATAACCCCA GCAGTGAAAG TGCACTTAAT
    GAAGAATGGC AGTCAGATAA TAGTGATGGA GATATTGCCA GTGAATGTGA AGAATGTGAT
    AGTGTCTTCA GCCATTTGGA AGAGCTGAGA TACAACCTGG AACAGGAAAT AGGCTTTGAG
    AAATTAATTG AAGTTTATAA TAAAATAAAG GCCATACATG AAGATGAAGA TGAAAATATT
    GATATTTGTT CAAGCATAGT TCAGACTATT CTGGGAAATG AACACAAACA CTTATATGCC
    AAAGTACTTC ACCTGGTCAT GGCGGATGGA GCCTACCAGG AAGATAATGA CGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34989
    Clone ID Related Accession (Same CDS sequence) XM_015285383.1 , XM_015285383.2
    Accession Version XM_015285383.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3117bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase Nek1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285383.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTGCACAC TTAAGCATGC ATTTGAAGCT GGTAACATGA AGAACTTGGT ACTGAAGATA 
    ATCTCTGGAT CTTTCCCTCC TGTTTCTGTG CATTATTCCT ATGACCTACG TAATCTACTG
    TCACAGTTAT TTAAAAGGAA TCCTAGGGAT AGACCATCAG TGAACTCCAT ATTGGAGAAA
    AATTTCATAG CCAAACGGGT TGAAAAATTT CTCACACCGC AGCTTATTGC AGAAGAATTC
    AGTCACAAAG TGTTTCACAA GTTTGGACCA CATGCTGGAC CAGCTAAAAG ACCAGCTCAA
    GGACATATCT CGGCTTCAAT TGCACCAGTG CAGAAAATTA CCAAACCTGC TGCAAAATAC
    GGAGTGCCTT TAGCGATGAA GAAGTGTTAT GACGCACCCA AAAAGCTTCA TGAGAAAAAG
    TCATTGATTA AACTTAGGCA GGCCTTTCCA ACTCCAAAGA AGAAAATTAT TCCAGGAGAA
    GAAAGGAGGA AAATGTTTGA GGAACCTTCA AAAAAGAAAA GGTTAGAATT GCCTGAAAAA
    GAAAAACGCC AGAGAGATCA GATCAGTTTA CTGAAGGCAG AGGAAATGAG AAGATTGGAA
    AAAGAAAGGA TGGAACGAAT AAACAGAGCC AGGGAGCAAG GATGGAGGAA TGTGCTTGGT
    TCAGGTGGAA GTGGTGATGT TAAGGCACCA TATTATGGCG GTGGAGGTGG TGTTGGTCCT
    TTTCCTGCCT CTTTCAGAGG ACAGTATGAG CACTATCATG CTATTTTTGA CCAGATGCAA
    AAGGAAACCT TCAAAGTAAC TAAAGAGAGG CAAGCCAAAT GGAGGGCGGA GGTACAAGGC
    CAAGAAGCTG TTGAGAGAGG AATTCCACCT GGAGTACGTC CAGGAGTTCC TAAGGGGCCT
    GCAGGTCATC ACCATTTACC TGATGCTGAT GCAATTAGGA AAAAAGTGAA AAGGTTGAAG
    GAGGTGTCTA AACAAGCCAG TGCAAACAGG CAGAAAGGAT GGCTACCTGC AGAAAGAGCA
    AAGCAAGTTG AAGAATTCTG GCAACGAAAG AGGGAAGCCA TGCAAAACAA AGCTCGTGCT
    GAGGGACACA TGGGTACACT GCAAAACGTG GCAGATATCT ATGGAGGCAG GCCCATGTCT
    GCAAGAGGAA GGAAATCCAG AAACAAGGAA GAAGAGGAAT ATTTGGCAAG ATTAAGACAG
    ATACGACTAC AAAACTTCAA TGAGCGTCAA CAGATTAAAG CAAAACTTCG TGGAGAGAAG
    AATGAAGCTC CCAGTTCTGA TGGACAAGAG TCAAGTGAGG AAGCAGAAAT GAAACGCAAA
    AAGATAGAAG CACAGAAGGC CCAAGCAAAT GCTCGAGCTG CAGTTCTGAA AGAACAGTTA
    GAGCGAAAGA GGAAGGAGGC ATATGAAAGA GAGAAAAGAG CTTGGGAAGA ACATCTGATA
    GCAAAAGGAG TAAAGAATCC TAATGTGCCT TTTCATGTGG GAGCAGCAGG ACACACTCCT
    GTGCATGGAA TACAAGAGCC TGGAGTGTCT CCTCCTAAGC AACAGCTTCC AGTTAAGCCC
    ATCTCCATGA CTTCTGCTTT GAAAGAAGTT GGCGTGGATG GAAATGTAAC CGCTGCCCAG
    GAGGTTAAAG AGGAAATAAA AAAGCCAGAT CTTACAATGC AGAACAAGAG GCAGATACTG
    AAAAGATTGA ATCAAAATCT TAAAGCTCAG GAAGATGAGA AAGGAAGAAA GGACGATAAA
    AATATCTCTG AGTCAGATGG GTCTGAAGAT GGTAAAGAAG AGCAAGAAGA TAACCATCCA
    CTTCTGGGAG ATCGCAAAAG ATGGGCATCA AGGGCATCAG ATTTGGTAGT TCCTTTAGCT
    CAGTTATCAA TGGAAGATTC TCTCTCTGGA ACAGACCGGC AAACTATGGG GGAAGTAATC
    AGGCTAGATA TTGGGGAACC CCGCAGGAAA GTCTGGGGCA AAAGCCCTAC TGATTCGGTC
    CTGAAGATTC TAGGAGAAGC GGAGTTGCAG CTACCAACTG AATTACTGGA AGAAATAGAT
    GTAATAAATG AGCCTGATGA TTTGGAAGCA GTGATGTTGA AAGAAACAGA AGAAGATAAA
    AAGCACCAGA GCAAGGAATT TCCCATTGCT GTTAATGAAG TGTGGGTGAA AGAAAAACAG
    GATGAGGCAC AACAGGATGC AAGAAATGAA GCAGCTTTTG AGAAAGAAGT AGTGCTTGAT
    GAAGTGCAAT CACAAAATGA AGCTCCAGAA GAAACTTGTT CCAGTGACTC TCTGCAACCT
    GAACCGTTAT TCCAGAGAGT AATACAGCCC CCAGCTGTAC CTACAGCCTC ACCAGTTCTA
    TCATCTCAGT CTTCCCAAGA AGAGCCTTTT GTACCTCGTT CACGTTCCAT ATCGCCAGCA
    AAAAATAAAG GGAAAAGTTC ATTGTTTTTT GGACTTTCTA CAGGTCTGTT TGATGCAAAC
    AACCCAAAGA TGTTGAGAAC GTGTTCACTC CCGGATCTTT ACAAACTGTA CAGAACCTTA
    GTTGATGTTC CCAGCATAGC AGATGTACAG CATCAACACA GTCTTGAAAT AGATGATACG
    GAAGATGAGC AAGCCAAAGA AGGACCCTCT GATTCTGAGG ATATCATGTT TGGGGACGCC
    GATACAGATT TGCAGGAACT CCGGGCCTCA ATGGAACAAT TACTTAGGGA GCAGCCTAGT
    GAGGAATTAA GTGAAGAGGA GGAATCTACT TTAAAGGCTA ACGTCATCAA ATGCATGACA
    AATGGTACAG AGCTGGATGA AGCAGATGAA AATAACCCCA GCAGTGAAAG TGCACTTAAT
    GAAGAATGGC AGTCAGATAA TAGTGATGGA GATATTGCCA GTGAATGTGA AGAATGTGAT
    AGTGTCTTCA GCCATTTGGA AGAGCTGAGA TACAACCTGG AACAGGAAAT AGGCTTTGAG
    AAATTAATTG AAGTTTATAA TAAAATAAAG GCCATACATG AAGATGAAGA TGAAAATATT
    GATATTTGTT CAAGCATAGT TCAGACTATT CTGGGAAATG AACACAAACA CTTATATGCC
    AAAGTACTTC ACCTGGTCAT GGCGGATGGA GCCTACCAGG AAGATAATGA CGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140869.1
    CDS100..3216
    Translation

    Target ORF information:

    RefSeq Version XM_015285383.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285383.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTGCACAC TTAAGCATGC ATTTGAAGCT GGTAACATGA AGAACTTGGT ACTGAAGATA 
    ATCTCTGGAT CTTTCCCTCC TGTTTCTGTG CATTATTCCT ATGACCTACG TAATCTACTG
    TCACAGTTAT TTAAAAGGAA TCCTAGGGAT AGACCATCAG TGAACTCCAT ATTGGAGAAA
    AATTTCATAG CCAAACGGGT TGAAAAATTT CTCACACCGC AGCTTATTGC AGAAGAATTC
    AGTCACAAAG TGTTTCACAA GTTTGGACCA CATGCTGGAC CAGCTAAAAG ACCAGCTCAA
    GGACATATCT CGGCTTCAAT TGCACCAGTG CAGAAAATTA CCAAACCTGC TGCAAAATAC
    GGAGTGCCTT TAGCGATGAA GAAGTGTTAT GACGCACCCA AAAAGCTTCA TGAGAAAAAG
    TCATTGATTA AACTTAGGCA GGCCTTTCCA ACTCCAAAGA AGAAAATTAT TCCAGGAGAA
    GAAAGGAGGA AAATGTTTGA GGAACCTTCA AAAAAGAAAA GGTTAGAATT GCCTGAAAAA
    GAAAAACGCC AGAGAGATCA GATCAGTTTA CTGAAGGCAG AGGAAATGAG AAGATTGGAA
    AAAGAAAGGA TGGAACGAAT AAACAGAGCC AGGGAGCAAG GATGGAGGAA TGTGCTTGGT
    TCAGGTGGAA GTGGTGATGT TAAGGCACCA TATTATGGCG GTGGAGGTGG TGTTGGTCCT
    TTTCCTGCCT CTTTCAGAGG ACAGTATGAG CACTATCATG CTATTTTTGA CCAGATGCAA
    AAGGAAACCT TCAAAGTAAC TAAAGAGAGG CAAGCCAAAT GGAGGGCGGA GGTACAAGGC
    CAAGAAGCTG TTGAGAGAGG AATTCCACCT GGAGTACGTC CAGGAGTTCC TAAGGGGCCT
    GCAGGTCATC ACCATTTACC TGATGCTGAT GCAATTAGGA AAAAAGTGAA AAGGTTGAAG
    GAGGTGTCTA AACAAGCCAG TGCAAACAGG CAGAAAGGAT GGCTACCTGC AGAAAGAGCA
    AAGCAAGTTG AAGAATTCTG GCAACGAAAG AGGGAAGCCA TGCAAAACAA AGCTCGTGCT
    GAGGGACACA TGGGTACACT GCAAAACGTG GCAGATATCT ATGGAGGCAG GCCCATGTCT
    GCAAGAGGAA GGAAATCCAG AAACAAGGAA GAAGAGGAAT ATTTGGCAAG ATTAAGACAG
    ATACGACTAC AAAACTTCAA TGAGCGTCAA CAGATTAAAG CAAAACTTCG TGGAGAGAAG
    AATGAAGCTC CCAGTTCTGA TGGACAAGAG TCAAGTGAGG AAGCAGAAAT GAAACGCAAA
    AAGATAGAAG CACAGAAGGC CCAAGCAAAT GCTCGAGCTG CAGTTCTGAA AGAACAGTTA
    GAGCGAAAGA GGAAGGAGGC ATATGAAAGA GAGAAAAGAG CTTGGGAAGA ACATCTGATA
    GCAAAAGGAG TAAAGAATCC TAATGTGCCT TTTCATGTGG GAGCAGCAGG ACACACTCCT
    GTGCATGGAA TACAAGAGCC TGGAGTGTCT CCTCCTAAGC AACAGCTTCC AGTTAAGCCC
    ATCTCCATGA CTTCTGCTTT GAAAGAAGTT GGCGTGGATG GAAATGTAAC CGCTGCCCAG
    GAGGTTAAAG AGGAAATAAA AAAGCCAGAT CTTACAATGC AGAACAAGAG GCAGATACTG
    AAAAGATTGA ATCAAAATCT TAAAGCTCAG GAAGATGAGA AAGGAAGAAA GGACGATAAA
    AATATCTCTG AGTCAGATGG GTCTGAAGAT GGTAAAGAAG AGCAAGAAGA TAACCATCCA
    CTTCTGGGAG ATCGCAAAAG ATGGGCATCA AGGGCATCAG ATTTGGTAGT TCCTTTAGCT
    CAGTTATCAA TGGAAGATTC TCTCTCTGGA ACAGACCGGC AAACTATGGG GGAAGTAATC
    AGGCTAGATA TTGGGGAACC CCGCAGGAAA GTCTGGGGCA AAAGCCCTAC TGATTCGGTC
    CTGAAGATTC TAGGAGAAGC GGAGTTGCAG CTACCAACTG AATTACTGGA AGAAATAGAT
    GTAATAAATG AGCCTGATGA TTTGGAAGCA GTGATGTTGA AAGAAACAGA AGAAGATAAA
    AAGCACCAGA GCAAGGAATT TCCCATTGCT GTTAATGAAG TGTGGGTGAA AGAAAAACAG
    GATGAGGCAC AACAGGATGC AAGAAATGAA GCAGCTTTTG AGAAAGAAGT AGTGCTTGAT
    GAAGTGCAAT CACAAAATGA AGCTCCAGAA GAAACTTGTT CCAGTGACTC TCTGCAACCT
    GAACCGTTAT TCCAGAGAGT AATACAGCCC CCAGCTGTAC CTACAGCCTC ACCAGTTCTA
    TCATCTCAGT CTTCCCAAGA AGAGCCTTTT GTACCTCGTT CACGTTCCAT ATCGCCAGCA
    AAAAATAAAG GGAAAAGTTC ATTGTTTTTT GGACTTTCTA CAGGTCTGTT TGATGCAAAC
    AACCCAAAGA TGTTGAGAAC GTGTTCACTC CCGGATCTTT ACAAACTGTA CAGAACCTTA
    GTTGATGTTC CCAGCATAGC AGATGTACAG CATCAACACA GTCTTGAAAT AGATGATACG
    GAAGATGAGC AAGCCAAAGA AGGACCCTCT GATTCTGAGG ATATCATGTT TGGGGACGCC
    GATACAGATT TGCAGGAACT CCGGGCCTCA ATGGAACAAT TACTTAGGGA GCAGCCTAGT
    GAGGAATTAA GTGAAGAGGA GGAATCTACT TTAAAGGCTA ACGTCATCAA ATGCATGACA
    AATGGTACAG AGCTGGATGA AGCAGATGAA AATAACCCCA GCAGTGAAAG TGCACTTAAT
    GAAGAATGGC AGTCAGATAA TAGTGATGGA GATATTGCCA GTGAATGTGA AGAATGTGAT
    AGTGTCTTCA GCCATTTGGA AGAGCTGAGA TACAACCTGG AACAGGAAAT AGGCTTTGAG
    AAATTAATTG AAGTTTATAA TAAAATAAAG GCCATACATG AAGATGAAGA TGAAAATATT
    GATATTTGTT CAAGCATAGT TCAGACTATT CTGGGAAATG AACACAAACA CTTATATGCC
    AAAGTACTTC ACCTGGTCAT GGCGGATGGA GCCTACCAGG AAGATAATGA CGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa07767
    Clone ID Related Accession (Same CDS sequence) XM_015285374.2 , XM_015285375.1 , XM_015285376.1 , XM_420401.5 , XM_420401.6 , XM_015285374.1 , XM_015285376.2 , XM_015285375.2
    Accession Version XM_015285374.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3702bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase Nek1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285374.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
    GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG
    TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT
    CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG
    GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC
    TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT
    GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA
    ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT
    CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC
    AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG
    CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC
    CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA
    AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA
    CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT
    CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT
    TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG
    ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT
    AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG
    TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA
    GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA
    CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT
    GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC
    AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA
    GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG
    AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT
    TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA
    GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA
    TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT
    ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA
    TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC
    TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT
    TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG
    AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG
    GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG
    AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA
    GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT
    GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA
    ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA
    AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA
    GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC
    AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA
    GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG
    GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA
    GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT
    GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG
    GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG
    GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA
    AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG
    AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC
    CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA
    AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG
    AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC
    ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC
    AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG
    GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA
    GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG
    GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA
    GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT
    TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT
    TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC
    ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG
    GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140860.1
    CDS1003..4704
    Translation

    Target ORF information:

    RefSeq Version XM_015285374.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285374.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
    GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG
    TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT
    CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG
    GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC
    TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT
    GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA
    ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT
    CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC
    AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG
    CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC
    CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA
    AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA
    CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT
    CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT
    TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG
    ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT
    AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG
    TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA
    GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA
    CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT
    GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC
    AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA
    GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG
    AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT
    TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA
    GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA
    TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT
    ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA
    TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC
    TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT
    TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG
    AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG
    GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG
    AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA
    GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT
    GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA
    ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA
    AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA
    GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC
    AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA
    GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG
    GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA
    GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT
    GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG
    GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG
    GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA
    AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG
    AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC
    CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA
    AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG
    AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC
    ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC
    AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG
    GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA
    GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG
    GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA
    GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT
    TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT
    TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC
    ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG
    GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa07767
    Clone ID Related Accession (Same CDS sequence) XM_015285374.2 , XM_015285375.1 , XM_015285376.1 , XM_420401.5 , XM_420401.6 , XM_015285374.1 , XM_015285376.2 , XM_015285375.2
    Accession Version XM_015285375.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3702bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase Nek1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455855.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
    GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG
    TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT
    CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG
    GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC
    TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT
    GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA
    ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT
    CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC
    AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG
    CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC
    CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA
    AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA
    CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT
    CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT
    TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG
    ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT
    AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG
    TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA
    GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA
    CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT
    GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC
    AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA
    GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG
    AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT
    TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA
    GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA
    TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT
    ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA
    TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC
    TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT
    TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG
    AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG
    GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG
    AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA
    GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT
    GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA
    ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA
    AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA
    GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC
    AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA
    GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG
    GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA
    GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT
    GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG
    GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG
    GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA
    AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG
    AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC
    CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA
    AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG
    AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC
    ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC
    AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG
    GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA
    GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG
    GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA
    GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT
    TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT
    TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC
    ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG
    GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140861.1
    CDS130..3831
    Translation

    Target ORF information:

    RefSeq Version XM_015285375.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285375.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
    GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG
    TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT
    CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG
    GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC
    TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT
    GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA
    ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT
    CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC
    AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG
    CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC
    CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA
    AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA
    CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT
    CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT
    TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG
    ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT
    AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG
    TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA
    GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA
    CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT
    GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC
    AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA
    GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG
    AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT
    TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA
    GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA
    TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT
    ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA
    TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC
    TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT
    TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG
    AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG
    GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG
    AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA
    GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT
    GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA
    ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA
    AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA
    GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC
    AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA
    GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG
    GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA
    GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT
    GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG
    GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG
    GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA
    AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG
    AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC
    CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA
    AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG
    AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC
    ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC
    AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG
    GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA
    GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG
    GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA
    GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT
    TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT
    TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC
    ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG
    GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa07767
    Clone ID Related Accession (Same CDS sequence) XM_015285374.2 , XM_015285375.1 , XM_015285376.1 , XM_420401.5 , XM_420401.6 , XM_015285374.1 , XM_015285376.2 , XM_015285375.2
    Accession Version XM_015285376.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3702bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase Nek1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455855.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
    GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG
    TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT
    CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG
    GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC
    TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT
    GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA
    ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT
    CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC
    AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG
    CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC
    CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA
    AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA
    CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT
    CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT
    TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG
    ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT
    AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG
    TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA
    GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA
    CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT
    GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC
    AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA
    GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG
    AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT
    TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA
    GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA
    TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT
    ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA
    TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC
    TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT
    TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG
    AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG
    GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG
    AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA
    GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT
    GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA
    ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA
    AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA
    GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC
    AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA
    GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG
    GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA
    GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT
    GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG
    GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG
    GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA
    AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG
    AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC
    CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA
    AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG
    AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC
    ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC
    AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG
    GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA
    GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG
    GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA
    GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT
    TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT
    TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC
    ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG
    GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140862.1
    CDS834..4535
    Translation

    Target ORF information:

    RefSeq Version XM_015285376.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285376.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
    GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG
    TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT
    CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG
    GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC
    TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT
    GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA
    ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT
    CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC
    AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG
    CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC
    CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA
    AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA
    CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT
    CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT
    TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG
    ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT
    AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG
    TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA
    GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA
    CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT
    GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC
    AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA
    GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG
    AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT
    TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA
    GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA
    TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT
    ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA
    TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC
    TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT
    TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG
    AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG
    GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG
    AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA
    GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT
    GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA
    ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA
    AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA
    GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC
    AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA
    GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG
    GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA
    GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT
    GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG
    GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG
    GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA
    AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG
    AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC
    CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA
    AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG
    AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC
    ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC
    AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG
    GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA
    GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG
    GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA
    GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT
    TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT
    TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC
    ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG
    GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa07767
    Clone ID Related Accession (Same CDS sequence) XM_015285374.2 , XM_015285375.1 , XM_015285376.1 , XM_420401.5 , XM_420401.6 , XM_015285374.1 , XM_015285376.2 , XM_015285375.2
    Accession Version XM_420401.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3702bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase Nek1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455855.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_420401.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
    GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG
    TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT
    CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG
    GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC
    TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT
    GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA
    ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT
    CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC
    AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG
    CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC
    CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA
    AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA
    CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT
    CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT
    TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG
    ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT
    AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG
    TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA
    GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA
    CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT
    GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC
    AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA
    GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG
    AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT
    TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA
    GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA
    TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT
    ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA
    TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC
    TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT
    TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG
    AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG
    GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG
    AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA
    GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT
    GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA
    ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA
    AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA
    GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC
    AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA
    GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG
    GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA
    GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT
    GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG
    GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG
    GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA
    AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG
    AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC
    CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA
    AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG
    AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC
    ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC
    AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG
    GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA
    GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG
    GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA
    GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT
    TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT
    TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC
    ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG
    GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_420401.3
    CDS182..3883
    Translation

    Target ORF information:

    RefSeq Version XM_420401.5
    Organism Gallus gallus(chicken)
    Definition Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_420401.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
    GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG
    TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT
    CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG
    GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC
    TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT
    GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA
    ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT
    CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC
    AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG
    CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC
    CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA
    AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA
    CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT
    CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT
    TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG
    ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT
    AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG
    TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA
    GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA
    CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT
    GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC
    AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA
    GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG
    AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT
    TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA
    GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA
    TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT
    ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA
    TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC
    TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT
    TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG
    AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG
    GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG
    AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA
    GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT
    GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA
    ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA
    AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA
    GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC
    AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA
    GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG
    GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA
    GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT
    GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG
    GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG
    GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA
    AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG
    AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC
    CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA
    AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG
    AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC
    ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC
    AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG
    GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA
    GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG
    GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA
    GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT
    TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT
    TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC
    ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG
    GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa07767
    Clone ID Related Accession (Same CDS sequence) XM_015285374.2 , XM_015285375.1 , XM_015285376.1 , XM_420401.5 , XM_420401.6 , XM_015285374.1 , XM_015285376.2 , XM_015285375.2
    Accession Version XM_420401.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3702bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase Nek1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_420401.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
    GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG
    TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT
    CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG
    GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC
    TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT
    GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA
    ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT
    CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC
    AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG
    CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC
    CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA
    AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA
    CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT
    CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT
    TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG
    ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT
    AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG
    TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA
    GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA
    CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT
    GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC
    AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA
    GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG
    AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT
    TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA
    GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA
    TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT
    ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA
    TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC
    TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT
    TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG
    AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG
    GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG
    AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA
    GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT
    GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA
    ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA
    AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA
    GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC
    AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA
    GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG
    GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA
    GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT
    GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG
    GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG
    GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA
    AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG
    AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC
    CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA
    AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG
    AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC
    ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC
    AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG
    GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA
    GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG
    GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA
    GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT
    TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT
    TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC
    ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG
    GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_420401.3
    CDS226..3927
    Translation

    Target ORF information:

    RefSeq Version XM_420401.6
    Organism Gallus gallus(chicken)
    Definition Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_420401.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
    GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG
    TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT
    CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG
    GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC
    TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT
    GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA
    ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT
    CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC
    AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG
    CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC
    CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA
    AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA
    CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT
    CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT
    TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG
    ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT
    AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG
    TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA
    GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA
    CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT
    GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC
    AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA
    GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG
    AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT
    TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA
    GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA
    TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT
    ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA
    TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC
    TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT
    TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG
    AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG
    GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG
    AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA
    GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT
    GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA
    ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA
    AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA
    GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC
    AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA
    GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG
    GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA
    GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT
    GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG
    GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG
    GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA
    AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG
    AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC
    CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA
    AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG
    AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC
    ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC
    AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG
    GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA
    GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG
    GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA
    GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT
    TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT
    TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC
    ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG
    GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa07767
    Clone ID Related Accession (Same CDS sequence) XM_015285374.2 , XM_015285375.1 , XM_015285376.1 , XM_420401.5 , XM_420401.6 , XM_015285374.1 , XM_015285376.2 , XM_015285375.2
    Accession Version XM_015285374.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3702bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase Nek1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455855.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
    GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG
    TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT
    CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG
    GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC
    TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT
    GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA
    ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT
    CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC
    AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG
    CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC
    CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA
    AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA
    CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT
    CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT
    TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG
    ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT
    AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG
    TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA
    GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA
    CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT
    GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC
    AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA
    GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG
    AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT
    TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA
    GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA
    TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT
    ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA
    TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC
    TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT
    TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG
    AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG
    GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG
    AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA
    GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT
    GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA
    ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA
    AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA
    GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC
    AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA
    GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG
    GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA
    GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT
    GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG
    GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG
    GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA
    AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG
    AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC
    CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA
    AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG
    AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC
    ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC
    AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG
    GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA
    GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG
    GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA
    GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT
    TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT
    TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC
    ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG
    GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140860.1
    CDS1005..4706
    Translation

    Target ORF information:

    RefSeq Version XM_015285374.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285374.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
    GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG
    TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT
    CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG
    GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC
    TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT
    GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA
    ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT
    CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC
    AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG
    CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC
    CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA
    AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA
    CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT
    CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT
    TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG
    ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT
    AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG
    TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA
    GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA
    CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT
    GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC
    AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA
    GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG
    AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT
    TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA
    GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA
    TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT
    ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA
    TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC
    TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT
    TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG
    AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG
    GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG
    AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA
    GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT
    GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA
    ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA
    AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA
    GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC
    AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA
    GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG
    GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA
    GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT
    GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG
    GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG
    GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA
    AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG
    AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC
    CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA
    AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG
    AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC
    ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC
    AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG
    GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA
    GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG
    GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA
    GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT
    TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT
    TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC
    ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG
    GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa07767
    Clone ID Related Accession (Same CDS sequence) XM_015285374.2 , XM_015285375.1 , XM_015285376.1 , XM_420401.5 , XM_420401.6 , XM_015285374.1 , XM_015285376.2 , XM_015285375.2
    Accession Version XM_015285376.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3702bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase Nek1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285376.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
    GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG
    TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT
    CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG
    GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC
    TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT
    GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA
    ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT
    CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC
    AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG
    CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC
    CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA
    AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA
    CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT
    CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT
    TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG
    ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT
    AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG
    TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA
    GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA
    CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT
    GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC
    AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA
    GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG
    AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT
    TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA
    GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA
    TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT
    ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA
    TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC
    TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT
    TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG
    AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG
    GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG
    AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA
    GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT
    GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA
    ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA
    AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA
    GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC
    AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA
    GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG
    GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA
    GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT
    GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG
    GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG
    GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA
    AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG
    AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC
    CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA
    AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG
    AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC
    ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC
    AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG
    GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA
    GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG
    GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA
    GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT
    TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT
    TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC
    ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG
    GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140862.1
    CDS832..4533
    Translation

    Target ORF information:

    RefSeq Version XM_015285376.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285376.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
    GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG
    TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT
    CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG
    GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC
    TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT
    GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA
    ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT
    CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC
    AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG
    CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC
    CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA
    AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA
    CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT
    CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT
    TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG
    ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT
    AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG
    TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA
    GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA
    CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT
    GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC
    AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA
    GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG
    AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT
    TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA
    GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA
    TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT
    ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA
    TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC
    TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT
    TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG
    AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG
    GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG
    AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA
    GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT
    GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA
    ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA
    AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA
    GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC
    AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA
    GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG
    GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA
    GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT
    GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG
    GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG
    GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA
    AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG
    AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC
    CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA
    AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG
    AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC
    ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC
    AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG
    GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA
    GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG
    GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA
    GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT
    TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT
    TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC
    ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG
    GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa07767
    Clone ID Related Accession (Same CDS sequence) XM_015285374.2 , XM_015285375.1 , XM_015285376.1 , XM_420401.5 , XM_420401.6 , XM_015285374.1 , XM_015285376.2 , XM_015285375.2
    Accession Version XM_015285375.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3702bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase Nek1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285375.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
    GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG
    TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT
    CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG
    GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC
    TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT
    GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA
    ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT
    CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC
    AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG
    CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC
    CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA
    AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA
    CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT
    CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT
    TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG
    ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT
    AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG
    TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA
    GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA
    CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT
    GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC
    AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA
    GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG
    AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT
    TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA
    GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA
    TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT
    ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA
    TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC
    TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT
    TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG
    AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG
    GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG
    AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA
    GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT
    GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA
    ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA
    AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA
    GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC
    AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA
    GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG
    GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA
    GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT
    GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG
    GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG
    GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA
    AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG
    AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC
    CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA
    AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG
    AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC
    ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC
    AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG
    GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA
    GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG
    GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA
    GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT
    TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT
    TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC
    ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG
    GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140861.1
    CDS216..3917
    Translation

    Target ORF information:

    RefSeq Version XM_015285375.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285375.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
    GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG
    TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT
    CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG
    GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC
    TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT
    GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA
    ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT
    CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC
    AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG
    CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC
    CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA
    AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA
    CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT
    CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT
    TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG
    ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT
    AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG
    TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA
    GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA
    CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT
    GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC
    AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA
    GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG
    AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT
    TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA
    GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA
    TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGGT
    ACACTGCAAA ACGTGGCAGA TATCTATGGA GGCAGGCCCA TGTCTGCAAG AGGAAGGAAA
    TCCAGAAACA AGGAAGAAGA GGAATATTTG GCAAGATTAA GACAGATACG ACTACAAAAC
    TTCAATGAGC GTCAACAGAT TAAAGCAAAA CTTCGTGGAG AGAAGAATGA AGCTCCCAGT
    TCTGATGGAC AAGAGTCAAG TGAGGAAGCA GAAATGAAAC GCAAAAAGAT AGAAGCACAG
    AAGGCCCAAG CAAATGCTCG AGCTGCAGTT CTGAAAGAAC AGTTAGAGCG AAAGAGGAAG
    GAGGCATATG AAAGAGAGAA AAGAGCTTGG GAAGAACATC TGATAGCAAA AGGAGTAAAG
    AATCCTAATG TGCCTTTTCA TGTGGGAGCA GCAGGACACA CTCCTGTGCA TGGAATACAA
    GAGCCTGGAG TGTCTCCTCC TAAGCAACAG CTTCCAGTTA AGCCCATCTC CATGACTTCT
    GCTTTGAAAG AAGTTGGCGT GGATGGAAAT GTAACCGCTG CCCAGGAGGT TAAAGAGGAA
    ATAAAAAAGC CAGATCTTAC AATGCAGAAC AAGAGGCAGA TACTGAAAAG ATTGAATCAA
    AATCTTAAAG CTCAGGAAGA TGAGAAAGGA AGAAAGGACG ATAAAAATAT CTCTGAGTCA
    GATGGGTCTG AAGATGGTAA AGAAGAGCAA GAAGATAACC ATCCACTTCT GGGAGATCGC
    AAAAGATGGG CATCAAGGGC ATCAGATTTG GTAGTTCCTT TAGCTCAGTT ATCAATGGAA
    GATTCTCTCT CTGGAACAGA CCGGCAAACT ATGGGGGAAG TAATCAGGCT AGATATTGGG
    GAACCCCGCA GGAAAGTCTG GGGCAAAAGC CCTACTGATT CGGTCCTGAA GATTCTAGGA
    GAAGCGGAGT TGCAGCTACC AACTGAATTA CTGGAAGAAA TAGATGTAAT AAATGAGCCT
    GATGATTTGG AAGCAGTGAT GTTGAAAGAA ACAGAAGAAG ATAAAAAGCA CCAGAGCAAG
    GAATTTCCCA TTGCTGTTAA TGAAGTGTGG GTGAAAGAAA AACAGGATGA GGCACAACAG
    GATGCAAGAA ATGAAGCAGC TTTTGAGAAA GAAGTAGTGC TTGATGAAGT GCAATCACAA
    AATGAAGCTC CAGAAGAAAC TTGTTCCAGT GACTCTCTGC AACCTGAACC GTTATTCCAG
    AGAGTAATAC AGCCCCCAGC TGTACCTACA GCCTCACCAG TTCTATCATC TCAGTCTTCC
    CAAGAAGAGC CTTTTGTACC TCGTTCACGT TCCATATCGC CAGCAAAAAA TAAAGGGAAA
    AGTTCATTGT TTTTTGGACT TTCTACAGGT CTGTTTGATG CAAACAACCC AAAGATGTTG
    AGAACGTGTT CACTCCCGGA TCTTTACAAA CTGTACAGAA CCTTAGTTGA TGTTCCCAGC
    ATAGCAGATG TACAGCATCA ACACAGTCTT GAAATAGATG ATACGGAAGA TGAGCAAGCC
    AAAGAAGGAC CCTCTGATTC TGAGGATATC ATGTTTGGGG ACGCCGATAC AGATTTGCAG
    GAACTCCGGG CCTCAATGGA ACAATTACTT AGGGAGCAGC CTAGTGAGGA ATTAAGTGAA
    GAGGAGGAAT CTACTTTAAA GGCTAACGTC ATCAAATGCA TGACAAATGG TACAGAGCTG
    GATGAAGCAG ATGAAAATAA CCCCAGCAGT GAAAGTGCAC TTAATGAAGA ATGGCAGTCA
    GATAATAGTG ATGGAGATAT TGCCAGTGAA TGTGAAGAAT GTGATAGTGT CTTCAGCCAT
    TTGGAAGAGC TGAGATACAA CCTGGAACAG GAAATAGGCT TTGAGAAATT AATTGAAGTT
    TATAATAAAA TAAAGGCCAT ACATGAAGAT GAAGATGAAA ATATTGATAT TTGTTCAAGC
    ATAGTTCAGA CTATTCTGGG AAATGAACAC AAACACTTAT ATGCCAAAGT ACTTCACCTG
    GTCATGGCGG ATGGAGCCTA CCAGGAAGAT AATGACGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa07768
    Clone ID Related Accession (Same CDS sequence) XM_015285379.1 , XM_015285379.2
    Accession Version XM_015285379.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3618bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase Nek1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455855.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
    GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG
    TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT
    CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG
    GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC
    TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT
    GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA
    ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT
    CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC
    AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG
    CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC
    CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA
    AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA
    CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT
    CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT
    TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG
    ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT
    AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG
    TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA
    GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA
    CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT
    GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC
    AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA
    GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG
    AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT
    TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA
    GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA
    TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGAA
    TATTTGGCAA GATTAAGACA GATACGACTA CAAAACTTCA ATGAGCGTCA ACAGATTAAA
    GCAAAACTTC GTGGAGAGAA GAATGAAGCT CCCAGTTCTG ATGGACAAGA GTCAAGTGAG
    GAAGCAGAAA TGAAACGCAA AAAGATAGAA GCACAGAAGG CCCAAGCAAA TGCTCGAGCT
    GCAGTTCTGA AAGAACAGTT AGAGCGAAAG AGGAAGGAGG CATATGAAAG AGAGAAAAGA
    GCTTGGGAAG AACATCTGAT AGCAAAAGGA GTAAAGAATC CTAATGTGCC TTTTCATGTG
    GGAGCAGCAG GACACACTCC TGTGCATGGA ATACAAGAGC CTGGAGTGTC TCCTCCTAAG
    CAACAGCTTC CAGTTAAGCC CATCTCCATG ACTTCTGCTT TGAAAGAAGT TGGCGTGGAT
    GGAAATGTAA CCGCTGCCCA GGAGGTTAAA GAGGAAATAA AAAAGCCAGA TCTTACAATG
    CAGAACAAGA GGCAGATACT GAAAAGATTG AATCAAAATC TTAAAGCTCA GGAAGATGAG
    AAAGGAAGAA AGGACGATAA AAATATCTCT GAGTCAGATG GGTCTGAAGA TGGTAAAGAA
    GAGCAAGAAG ATAACCATCC ACTTCTGGGA GATCGCAAAA GATGGGCATC AAGGGCATCA
    GATTTGGTAG TTCCTTTAGC TCAGTTATCA ATGGAAGATT CTCTCTCTGG AACAGACCGG
    CAAACTATGG GGGAAGTAAT CAGGCTAGAT ATTGGGGAAC CCCGCAGGAA AGTCTGGGGC
    AAAAGCCCTA CTGATTCGGT CCTGAAGATT CTAGGAGAAG CGGAGTTGCA GCTACCAACT
    GAATTACTGG AAGAAATAGA TGTAATAAAT GAGCCTGATG ATTTGGAAGC AGTGATGTTG
    AAAGAAACAG AAGAAGATAA AAAGCACCAG AGCAAGGAAT TTCCCATTGC TGTTAATGAA
    GTGTGGGTGA AAGAAAAACA GGATGAGGCA CAACAGGATG CAAGAAATGA AGCAGCTTTT
    GAGAAAGAAG TAGTGCTTGA TGAAGTGCAA TCACAAAATG AAGCTCCAGA AGAAACTTGT
    TCCAGTGACT CTCTGCAACC TGAACCGTTA TTCCAGAGAG TAATACAGCC CCCAGCTGTA
    CCTACAGCCT CACCAGTTCT ATCATCTCAG TCTTCCCAAG AAGAGCCTTT TGTACCTCGT
    TCACGTTCCA TATCGCCAGC AAAAAATAAA GGGAAAAGTT CATTGTTTTT TGGACTTTCT
    ACAGGTCTGT TTGATGCAAA CAACCCAAAG ATGTTGAGAA CGTGTTCACT CCCGGATCTT
    TACAAACTGT ACAGAACCTT AGTTGATGTT CCCAGCATAG CAGATGTACA GCATCAACAC
    AGTCTTGAAA TAGATGATAC GGAAGATGAG CAAGCCAAAG AAGGACCCTC TGATTCTGAG
    GATATCATGT TTGGGGACGC CGATACAGAT TTGCAGGAAC TCCGGGCCTC AATGGAACAA
    TTACTTAGGG AGCAGCCTAG TGAGGAATTA AGTGAAGAGG AGGAATCTAC TTTAAAGGCT
    AACGTCATCA AATGCATGAC AAATGGTACA GAGCTGGATG AAGCAGATGA AAATAACCCC
    AGCAGTGAAA GTGCACTTAA TGAAGAATGG CAGTCAGATA ATAGTGATGG AGATATTGCC
    AGTGAATGTG AAGAATGTGA TAGTGTCTTC AGCCATTTGG AAGAGCTGAG ATACAACCTG
    GAACAGGAAA TAGGCTTTGA GAAATTAATT GAAGTTTATA ATAAAATAAA GGCCATACAT
    GAAGATGAAG ATGAAAATAT TGATATTTGT TCAAGCATAG TTCAGACTAT TCTGGGAAAT
    GAACACAAAC ACTTATATGC CAAAGTACTT CACCTGGTCA TGGCGGATGG AGCCTACCAG
    GAAGATAATG ACGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140865.1
    CDS1007..4624
    Translation

    Target ORF information:

    RefSeq Version XM_015285379.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285379.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
    GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG
    TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT
    CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG
    GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC
    TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT
    GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA
    ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT
    CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC
    AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG
    CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC
    CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA
    AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA
    CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT
    CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT
    TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG
    ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT
    AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG
    TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA
    GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA
    CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT
    GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC
    AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA
    GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG
    AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT
    TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA
    GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA
    TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGAA
    TATTTGGCAA GATTAAGACA GATACGACTA CAAAACTTCA ATGAGCGTCA ACAGATTAAA
    GCAAAACTTC GTGGAGAGAA GAATGAAGCT CCCAGTTCTG ATGGACAAGA GTCAAGTGAG
    GAAGCAGAAA TGAAACGCAA AAAGATAGAA GCACAGAAGG CCCAAGCAAA TGCTCGAGCT
    GCAGTTCTGA AAGAACAGTT AGAGCGAAAG AGGAAGGAGG CATATGAAAG AGAGAAAAGA
    GCTTGGGAAG AACATCTGAT AGCAAAAGGA GTAAAGAATC CTAATGTGCC TTTTCATGTG
    GGAGCAGCAG GACACACTCC TGTGCATGGA ATACAAGAGC CTGGAGTGTC TCCTCCTAAG
    CAACAGCTTC CAGTTAAGCC CATCTCCATG ACTTCTGCTT TGAAAGAAGT TGGCGTGGAT
    GGAAATGTAA CCGCTGCCCA GGAGGTTAAA GAGGAAATAA AAAAGCCAGA TCTTACAATG
    CAGAACAAGA GGCAGATACT GAAAAGATTG AATCAAAATC TTAAAGCTCA GGAAGATGAG
    AAAGGAAGAA AGGACGATAA AAATATCTCT GAGTCAGATG GGTCTGAAGA TGGTAAAGAA
    GAGCAAGAAG ATAACCATCC ACTTCTGGGA GATCGCAAAA GATGGGCATC AAGGGCATCA
    GATTTGGTAG TTCCTTTAGC TCAGTTATCA ATGGAAGATT CTCTCTCTGG AACAGACCGG
    CAAACTATGG GGGAAGTAAT CAGGCTAGAT ATTGGGGAAC CCCGCAGGAA AGTCTGGGGC
    AAAAGCCCTA CTGATTCGGT CCTGAAGATT CTAGGAGAAG CGGAGTTGCA GCTACCAACT
    GAATTACTGG AAGAAATAGA TGTAATAAAT GAGCCTGATG ATTTGGAAGC AGTGATGTTG
    AAAGAAACAG AAGAAGATAA AAAGCACCAG AGCAAGGAAT TTCCCATTGC TGTTAATGAA
    GTGTGGGTGA AAGAAAAACA GGATGAGGCA CAACAGGATG CAAGAAATGA AGCAGCTTTT
    GAGAAAGAAG TAGTGCTTGA TGAAGTGCAA TCACAAAATG AAGCTCCAGA AGAAACTTGT
    TCCAGTGACT CTCTGCAACC TGAACCGTTA TTCCAGAGAG TAATACAGCC CCCAGCTGTA
    CCTACAGCCT CACCAGTTCT ATCATCTCAG TCTTCCCAAG AAGAGCCTTT TGTACCTCGT
    TCACGTTCCA TATCGCCAGC AAAAAATAAA GGGAAAAGTT CATTGTTTTT TGGACTTTCT
    ACAGGTCTGT TTGATGCAAA CAACCCAAAG ATGTTGAGAA CGTGTTCACT CCCGGATCTT
    TACAAACTGT ACAGAACCTT AGTTGATGTT CCCAGCATAG CAGATGTACA GCATCAACAC
    AGTCTTGAAA TAGATGATAC GGAAGATGAG CAAGCCAAAG AAGGACCCTC TGATTCTGAG
    GATATCATGT TTGGGGACGC CGATACAGAT TTGCAGGAAC TCCGGGCCTC AATGGAACAA
    TTACTTAGGG AGCAGCCTAG TGAGGAATTA AGTGAAGAGG AGGAATCTAC TTTAAAGGCT
    AACGTCATCA AATGCATGAC AAATGGTACA GAGCTGGATG AAGCAGATGA AAATAACCCC
    AGCAGTGAAA GTGCACTTAA TGAAGAATGG CAGTCAGATA ATAGTGATGG AGATATTGCC
    AGTGAATGTG AAGAATGTGA TAGTGTCTTC AGCCATTTGG AAGAGCTGAG ATACAACCTG
    GAACAGGAAA TAGGCTTTGA GAAATTAATT GAAGTTTATA ATAAAATAAA GGCCATACAT
    GAAGATGAAG ATGAAAATAT TGATATTTGT TCAAGCATAG TTCAGACTAT TCTGGGAAAT
    GAACACAAAC ACTTATATGC CAAAGTACTT CACCTGGTCA TGGCGGATGG AGCCTACCAG
    GAAGATAATG ACGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa07768
    Clone ID Related Accession (Same CDS sequence) XM_015285379.1 , XM_015285379.2
    Accession Version XM_015285379.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3618bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase Nek1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285379.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
    GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG
    TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT
    CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG
    GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC
    TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT
    GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA
    ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT
    CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC
    AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG
    CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC
    CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA
    AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA
    CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT
    CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT
    TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG
    ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT
    AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG
    TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA
    GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA
    CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT
    GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC
    AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA
    GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG
    AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT
    TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA
    GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA
    TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGAA
    TATTTGGCAA GATTAAGACA GATACGACTA CAAAACTTCA ATGAGCGTCA ACAGATTAAA
    GCAAAACTTC GTGGAGAGAA GAATGAAGCT CCCAGTTCTG ATGGACAAGA GTCAAGTGAG
    GAAGCAGAAA TGAAACGCAA AAAGATAGAA GCACAGAAGG CCCAAGCAAA TGCTCGAGCT
    GCAGTTCTGA AAGAACAGTT AGAGCGAAAG AGGAAGGAGG CATATGAAAG AGAGAAAAGA
    GCTTGGGAAG AACATCTGAT AGCAAAAGGA GTAAAGAATC CTAATGTGCC TTTTCATGTG
    GGAGCAGCAG GACACACTCC TGTGCATGGA ATACAAGAGC CTGGAGTGTC TCCTCCTAAG
    CAACAGCTTC CAGTTAAGCC CATCTCCATG ACTTCTGCTT TGAAAGAAGT TGGCGTGGAT
    GGAAATGTAA CCGCTGCCCA GGAGGTTAAA GAGGAAATAA AAAAGCCAGA TCTTACAATG
    CAGAACAAGA GGCAGATACT GAAAAGATTG AATCAAAATC TTAAAGCTCA GGAAGATGAG
    AAAGGAAGAA AGGACGATAA AAATATCTCT GAGTCAGATG GGTCTGAAGA TGGTAAAGAA
    GAGCAAGAAG ATAACCATCC ACTTCTGGGA GATCGCAAAA GATGGGCATC AAGGGCATCA
    GATTTGGTAG TTCCTTTAGC TCAGTTATCA ATGGAAGATT CTCTCTCTGG AACAGACCGG
    CAAACTATGG GGGAAGTAAT CAGGCTAGAT ATTGGGGAAC CCCGCAGGAA AGTCTGGGGC
    AAAAGCCCTA CTGATTCGGT CCTGAAGATT CTAGGAGAAG CGGAGTTGCA GCTACCAACT
    GAATTACTGG AAGAAATAGA TGTAATAAAT GAGCCTGATG ATTTGGAAGC AGTGATGTTG
    AAAGAAACAG AAGAAGATAA AAAGCACCAG AGCAAGGAAT TTCCCATTGC TGTTAATGAA
    GTGTGGGTGA AAGAAAAACA GGATGAGGCA CAACAGGATG CAAGAAATGA AGCAGCTTTT
    GAGAAAGAAG TAGTGCTTGA TGAAGTGCAA TCACAAAATG AAGCTCCAGA AGAAACTTGT
    TCCAGTGACT CTCTGCAACC TGAACCGTTA TTCCAGAGAG TAATACAGCC CCCAGCTGTA
    CCTACAGCCT CACCAGTTCT ATCATCTCAG TCTTCCCAAG AAGAGCCTTT TGTACCTCGT
    TCACGTTCCA TATCGCCAGC AAAAAATAAA GGGAAAAGTT CATTGTTTTT TGGACTTTCT
    ACAGGTCTGT TTGATGCAAA CAACCCAAAG ATGTTGAGAA CGTGTTCACT CCCGGATCTT
    TACAAACTGT ACAGAACCTT AGTTGATGTT CCCAGCATAG CAGATGTACA GCATCAACAC
    AGTCTTGAAA TAGATGATAC GGAAGATGAG CAAGCCAAAG AAGGACCCTC TGATTCTGAG
    GATATCATGT TTGGGGACGC CGATACAGAT TTGCAGGAAC TCCGGGCCTC AATGGAACAA
    TTACTTAGGG AGCAGCCTAG TGAGGAATTA AGTGAAGAGG AGGAATCTAC TTTAAAGGCT
    AACGTCATCA AATGCATGAC AAATGGTACA GAGCTGGATG AAGCAGATGA AAATAACCCC
    AGCAGTGAAA GTGCACTTAA TGAAGAATGG CAGTCAGATA ATAGTGATGG AGATATTGCC
    AGTGAATGTG AAGAATGTGA TAGTGTCTTC AGCCATTTGG AAGAGCTGAG ATACAACCTG
    GAACAGGAAA TAGGCTTTGA GAAATTAATT GAAGTTTATA ATAAAATAAA GGCCATACAT
    GAAGATGAAG ATGAAAATAT TGATATTTGT TCAAGCATAG TTCAGACTAT TCTGGGAAAT
    GAACACAAAC ACTTATATGC CAAAGTACTT CACCTGGTCA TGGCGGATGG AGCCTACCAG
    GAAGATAATG ACGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140865.1
    CDS1004..4621
    Translation

    Target ORF information:

    RefSeq Version XM_015285379.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285379.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
    GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG
    TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT
    CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG
    GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC
    TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT
    GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA
    ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT
    CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC
    AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG
    CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC
    CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA
    AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA
    CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT
    CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT
    TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG
    ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT
    AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG
    TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA
    GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA
    CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT
    GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC
    AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA
    GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG
    AGAGGAATTC CACCTGGAGT ACGTCCAGGA GTTCCTAAGG GGCCTGCAGG TCATCACCAT
    TTACCTGATG CTGATGCAAT TAGGAAAAAA GTGAAAAGGT TGAAGGAGGT GTCTAAACAA
    GCCAGTGCAA ACAGGCAGAA AGGATGGCTA CCTGCAGAAA GAGCAAAGCA AGTTGAAGAA
    TTCTGGCAAC GAAAGAGGGA AGCCATGCAA AACAAAGCTC GTGCTGAGGG ACACATGGAA
    TATTTGGCAA GATTAAGACA GATACGACTA CAAAACTTCA ATGAGCGTCA ACAGATTAAA
    GCAAAACTTC GTGGAGAGAA GAATGAAGCT CCCAGTTCTG ATGGACAAGA GTCAAGTGAG
    GAAGCAGAAA TGAAACGCAA AAAGATAGAA GCACAGAAGG CCCAAGCAAA TGCTCGAGCT
    GCAGTTCTGA AAGAACAGTT AGAGCGAAAG AGGAAGGAGG CATATGAAAG AGAGAAAAGA
    GCTTGGGAAG AACATCTGAT AGCAAAAGGA GTAAAGAATC CTAATGTGCC TTTTCATGTG
    GGAGCAGCAG GACACACTCC TGTGCATGGA ATACAAGAGC CTGGAGTGTC TCCTCCTAAG
    CAACAGCTTC CAGTTAAGCC CATCTCCATG ACTTCTGCTT TGAAAGAAGT TGGCGTGGAT
    GGAAATGTAA CCGCTGCCCA GGAGGTTAAA GAGGAAATAA AAAAGCCAGA TCTTACAATG
    CAGAACAAGA GGCAGATACT GAAAAGATTG AATCAAAATC TTAAAGCTCA GGAAGATGAG
    AAAGGAAGAA AGGACGATAA AAATATCTCT GAGTCAGATG GGTCTGAAGA TGGTAAAGAA
    GAGCAAGAAG ATAACCATCC ACTTCTGGGA GATCGCAAAA GATGGGCATC AAGGGCATCA
    GATTTGGTAG TTCCTTTAGC TCAGTTATCA ATGGAAGATT CTCTCTCTGG AACAGACCGG
    CAAACTATGG GGGAAGTAAT CAGGCTAGAT ATTGGGGAAC CCCGCAGGAA AGTCTGGGGC
    AAAAGCCCTA CTGATTCGGT CCTGAAGATT CTAGGAGAAG CGGAGTTGCA GCTACCAACT
    GAATTACTGG AAGAAATAGA TGTAATAAAT GAGCCTGATG ATTTGGAAGC AGTGATGTTG
    AAAGAAACAG AAGAAGATAA AAAGCACCAG AGCAAGGAAT TTCCCATTGC TGTTAATGAA
    GTGTGGGTGA AAGAAAAACA GGATGAGGCA CAACAGGATG CAAGAAATGA AGCAGCTTTT
    GAGAAAGAAG TAGTGCTTGA TGAAGTGCAA TCACAAAATG AAGCTCCAGA AGAAACTTGT
    TCCAGTGACT CTCTGCAACC TGAACCGTTA TTCCAGAGAG TAATACAGCC CCCAGCTGTA
    CCTACAGCCT CACCAGTTCT ATCATCTCAG TCTTCCCAAG AAGAGCCTTT TGTACCTCGT
    TCACGTTCCA TATCGCCAGC AAAAAATAAA GGGAAAAGTT CATTGTTTTT TGGACTTTCT
    ACAGGTCTGT TTGATGCAAA CAACCCAAAG ATGTTGAGAA CGTGTTCACT CCCGGATCTT
    TACAAACTGT ACAGAACCTT AGTTGATGTT CCCAGCATAG CAGATGTACA GCATCAACAC
    AGTCTTGAAA TAGATGATAC GGAAGATGAG CAAGCCAAAG AAGGACCCTC TGATTCTGAG
    GATATCATGT TTGGGGACGC CGATACAGAT TTGCAGGAAC TCCGGGCCTC AATGGAACAA
    TTACTTAGGG AGCAGCCTAG TGAGGAATTA AGTGAAGAGG AGGAATCTAC TTTAAAGGCT
    AACGTCATCA AATGCATGAC AAATGGTACA GAGCTGGATG AAGCAGATGA AAATAACCCC
    AGCAGTGAAA GTGCACTTAA TGAAGAATGG CAGTCAGATA ATAGTGATGG AGATATTGCC
    AGTGAATGTG AAGAATGTGA TAGTGTCTTC AGCCATTTGG AAGAGCTGAG ATACAACCTG
    GAACAGGAAA TAGGCTTTGA GAAATTAATT GAAGTTTATA ATAAAATAAA GGCCATACAT
    GAAGATGAAG ATGAAAATAT TGATATTTGT TCAAGCATAG TTCAGACTAT TCTGGGAAAT
    GAACACAAAC ACTTATATGC CAAAGTACTT CACCTGGTCA TGGCGGATGG AGCCTACCAG
    GAAGATAATG ACGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa07769
    Clone ID Related Accession (Same CDS sequence) XM_015285381.2 , XM_015285381.1
    Accession Version XM_015285381.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3570bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase Nek1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285381.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
    GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG
    TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT
    CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG
    GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC
    TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT
    GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA
    ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT
    CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC
    AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG
    CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC
    CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA
    AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA
    CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT
    CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT
    TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG
    ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT
    AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG
    TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA
    GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA
    CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT
    GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC
    AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA
    GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG
    AGGCAGAAAG GATGGCTACC TGCAGAAAGA GCAAAGCAAG TTGAAGAATT CTGGCAACGA
    AAGAGGGAAG CCATGCAAAA CAAAGCTCGT GCTGAGGGAC ACATGGGTAC ACTGCAAAAC
    GTGGCAGATA TCTATGGAGG CAGGCCCATG TCTGCAAGAG GAAGGAAATC CAGAAACAAG
    GAAGAAGAGG AATATTTGGC AAGATTAAGA CAGATACGAC TACAAAACTT CAATGAGCGT
    CAACAGATTA AAGCAAAACT TCGTGGAGAG AAGAATGAAG CTCCCAGTTC TGATGGACAA
    GAGTCAAGTG AGGAAGCAGA AATGAAACGC AAAAAGATAG AAGCACAGAA GGCCCAAGCA
    AATGCTCGAG CTGCAGTTCT GAAAGAACAG TTAGAGCGAA AGAGGAAGGA GGCATATGAA
    AGAGAGAAAA GAGCTTGGGA AGAACATCTG ATAGCAAAAG GAGTAAAGAA TCCTAATGTG
    CCTTTTCATG TGGGAGCAGC AGGACACACT CCTGTGCATG GAATACAAGA GCCTGGAGTG
    TCTCCTCCTA AGCAACAGCT TCCAGTTAAG CCCATCTCCA TGACTTCTGC TTTGAAAGAA
    GTTGGCGTGG ATGGAAATGT AACCGCTGCC CAGGAGGTTA AAGAGGAAAT AAAAAAGCCA
    GATCTTACAA TGCAGAACAA GAGGCAGATA CTGAAAAGAT TGAATCAAAA TCTTAAAGCT
    CAGGAAGATG AGAAAGGAAG AAAGGACGAT AAAAATATCT CTGAGTCAGA TGGGTCTGAA
    GATGGTAAAG AAGAGCAAGA AGATAACCAT CCACTTCTGG GAGATCGCAA AAGATGGGCA
    TCAAGGGCAT CAGATTTGGT AGTTCCTTTA GCTCAGTTAT CAATGGAAGA TTCTCTCTCT
    GGAACAGACC GGCAAACTAT GGGGGAAGTA ATCAGGCTAG ATATTGGGGA ACCCCGCAGG
    AAAGTCTGGG GCAAAAGCCC TACTGATTCG GTCCTGAAGA TTCTAGGAGA AGCGGAGTTG
    CAGCTACCAA CTGAATTACT GGAAGAAATA GATGTAATAA ATGAGCCTGA TGATTTGGAA
    GCAGTGATGT TGAAAGAAAC AGAAGAAGAT AAAAAGCACC AGAGCAAGGA ATTTCCCATT
    GCTGTTAATG AAGTGTGGGT GAAAGAAAAA CAGGATGAGG CACAACAGGA TGCAAGAAAT
    GAAGCAGCTT TTGAGAAAGA AGTAGTGCTT GATGAAGTGC AATCACAAAA TGAAGCTCCA
    GAAGAAACTT GTTCCAGTGA CTCTCTGCAA CCTGAACCGT TATTCCAGAG AGTAATACAG
    CCCCCAGCTG TACCTACAGC CTCACCAGTT CTATCATCTC AGTCTTCCCA AGAAGAGCCT
    TTTGTACCTC GTTCACGTTC CATATCGCCA GCAAAAAATA AAGGGAAAAG TTCATTGTTT
    TTTGGACTTT CTACAGGTCT GTTTGATGCA AACAACCCAA AGATGTTGAG AACGTGTTCA
    CTCCCGGATC TTTACAAACT GTACAGAACC TTAGTTGATG TTCCCAGCAT AGCAGATGTA
    CAGCATCAAC ACAGTCTTGA AATAGATGAT ACGGAAGATG AGCAAGCCAA AGAAGGACCC
    TCTGATTCTG AGGATATCAT GTTTGGGGAC GCCGATACAG ATTTGCAGGA ACTCCGGGCC
    TCAATGGAAC AATTACTTAG GGAGCAGCCT AGTGAGGAAT TAAGTGAAGA GGAGGAATCT
    ACTTTAAAGG CTAACGTCAT CAAATGCATG ACAAATGGTA CAGAGCTGGA TGAAGCAGAT
    GAAAATAACC CCAGCAGTGA AAGTGCACTT AATGAAGAAT GGCAGTCAGA TAATAGTGAT
    GGAGATATTG CCAGTGAATG TGAAGAATGT GATAGTGTCT TCAGCCATTT GGAAGAGCTG
    AGATACAACC TGGAACAGGA AATAGGCTTT GAGAAATTAA TTGAAGTTTA TAATAAAATA
    AAGGCCATAC ATGAAGATGA AGATGAAAAT ATTGATATTT GTTCAAGCAT AGTTCAGACT
    ATTCTGGGAA ATGAACACAA ACACTTATAT GCCAAAGTAC TTCACCTGGT CATGGCGGAT
    GGAGCCTACC AGGAAGATAA TGACGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140867.1
    CDS1004..4573
    Translation

    Target ORF information:

    RefSeq Version XM_015285381.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285381.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
    GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG
    TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT
    CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG
    GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC
    TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT
    GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA
    ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT
    CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC
    AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG
    CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC
    CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA
    AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA
    CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT
    CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT
    TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG
    ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT
    AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG
    TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA
    GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA
    CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT
    GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC
    AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA
    GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG
    AGGCAGAAAG GATGGCTACC TGCAGAAAGA GCAAAGCAAG TTGAAGAATT CTGGCAACGA
    AAGAGGGAAG CCATGCAAAA CAAAGCTCGT GCTGAGGGAC ACATGGGTAC ACTGCAAAAC
    GTGGCAGATA TCTATGGAGG CAGGCCCATG TCTGCAAGAG GAAGGAAATC CAGAAACAAG
    GAAGAAGAGG AATATTTGGC AAGATTAAGA CAGATACGAC TACAAAACTT CAATGAGCGT
    CAACAGATTA AAGCAAAACT TCGTGGAGAG AAGAATGAAG CTCCCAGTTC TGATGGACAA
    GAGTCAAGTG AGGAAGCAGA AATGAAACGC AAAAAGATAG AAGCACAGAA GGCCCAAGCA
    AATGCTCGAG CTGCAGTTCT GAAAGAACAG TTAGAGCGAA AGAGGAAGGA GGCATATGAA
    AGAGAGAAAA GAGCTTGGGA AGAACATCTG ATAGCAAAAG GAGTAAAGAA TCCTAATGTG
    CCTTTTCATG TGGGAGCAGC AGGACACACT CCTGTGCATG GAATACAAGA GCCTGGAGTG
    TCTCCTCCTA AGCAACAGCT TCCAGTTAAG CCCATCTCCA TGACTTCTGC TTTGAAAGAA
    GTTGGCGTGG ATGGAAATGT AACCGCTGCC CAGGAGGTTA AAGAGGAAAT AAAAAAGCCA
    GATCTTACAA TGCAGAACAA GAGGCAGATA CTGAAAAGAT TGAATCAAAA TCTTAAAGCT
    CAGGAAGATG AGAAAGGAAG AAAGGACGAT AAAAATATCT CTGAGTCAGA TGGGTCTGAA
    GATGGTAAAG AAGAGCAAGA AGATAACCAT CCACTTCTGG GAGATCGCAA AAGATGGGCA
    TCAAGGGCAT CAGATTTGGT AGTTCCTTTA GCTCAGTTAT CAATGGAAGA TTCTCTCTCT
    GGAACAGACC GGCAAACTAT GGGGGAAGTA ATCAGGCTAG ATATTGGGGA ACCCCGCAGG
    AAAGTCTGGG GCAAAAGCCC TACTGATTCG GTCCTGAAGA TTCTAGGAGA AGCGGAGTTG
    CAGCTACCAA CTGAATTACT GGAAGAAATA GATGTAATAA ATGAGCCTGA TGATTTGGAA
    GCAGTGATGT TGAAAGAAAC AGAAGAAGAT AAAAAGCACC AGAGCAAGGA ATTTCCCATT
    GCTGTTAATG AAGTGTGGGT GAAAGAAAAA CAGGATGAGG CACAACAGGA TGCAAGAAAT
    GAAGCAGCTT TTGAGAAAGA AGTAGTGCTT GATGAAGTGC AATCACAAAA TGAAGCTCCA
    GAAGAAACTT GTTCCAGTGA CTCTCTGCAA CCTGAACCGT TATTCCAGAG AGTAATACAG
    CCCCCAGCTG TACCTACAGC CTCACCAGTT CTATCATCTC AGTCTTCCCA AGAAGAGCCT
    TTTGTACCTC GTTCACGTTC CATATCGCCA GCAAAAAATA AAGGGAAAAG TTCATTGTTT
    TTTGGACTTT CTACAGGTCT GTTTGATGCA AACAACCCAA AGATGTTGAG AACGTGTTCA
    CTCCCGGATC TTTACAAACT GTACAGAACC TTAGTTGATG TTCCCAGCAT AGCAGATGTA
    CAGCATCAAC ACAGTCTTGA AATAGATGAT ACGGAAGATG AGCAAGCCAA AGAAGGACCC
    TCTGATTCTG AGGATATCAT GTTTGGGGAC GCCGATACAG ATTTGCAGGA ACTCCGGGCC
    TCAATGGAAC AATTACTTAG GGAGCAGCCT AGTGAGGAAT TAAGTGAAGA GGAGGAATCT
    ACTTTAAAGG CTAACGTCAT CAAATGCATG ACAAATGGTA CAGAGCTGGA TGAAGCAGAT
    GAAAATAACC CCAGCAGTGA AAGTGCACTT AATGAAGAAT GGCAGTCAGA TAATAGTGAT
    GGAGATATTG CCAGTGAATG TGAAGAATGT GATAGTGTCT TCAGCCATTT GGAAGAGCTG
    AGATACAACC TGGAACAGGA AATAGGCTTT GAGAAATTAA TTGAAGTTTA TAATAAAATA
    AAGGCCATAC ATGAAGATGA AGATGAAAAT ATTGATATTT GTTCAAGCAT AGTTCAGACT
    ATTCTGGGAA ATGAACACAA ACACTTATAT GCCAAAGTAC TTCACCTGGT CATGGCGGAT
    GGAGCCTACC AGGAAGATAA TGACGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa07769
    Clone ID Related Accession (Same CDS sequence) XM_015285381.2 , XM_015285381.1
    Accession Version XM_015285381.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3570bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase Nek1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455855.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
    GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG
    TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT
    CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG
    GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC
    TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT
    GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA
    ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT
    CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC
    AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG
    CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC
    CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA
    AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA
    CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT
    CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT
    TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG
    ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT
    AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG
    TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA
    GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA
    CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT
    GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC
    AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA
    GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG
    AGGCAGAAAG GATGGCTACC TGCAGAAAGA GCAAAGCAAG TTGAAGAATT CTGGCAACGA
    AAGAGGGAAG CCATGCAAAA CAAAGCTCGT GCTGAGGGAC ACATGGGTAC ACTGCAAAAC
    GTGGCAGATA TCTATGGAGG CAGGCCCATG TCTGCAAGAG GAAGGAAATC CAGAAACAAG
    GAAGAAGAGG AATATTTGGC AAGATTAAGA CAGATACGAC TACAAAACTT CAATGAGCGT
    CAACAGATTA AAGCAAAACT TCGTGGAGAG AAGAATGAAG CTCCCAGTTC TGATGGACAA
    GAGTCAAGTG AGGAAGCAGA AATGAAACGC AAAAAGATAG AAGCACAGAA GGCCCAAGCA
    AATGCTCGAG CTGCAGTTCT GAAAGAACAG TTAGAGCGAA AGAGGAAGGA GGCATATGAA
    AGAGAGAAAA GAGCTTGGGA AGAACATCTG ATAGCAAAAG GAGTAAAGAA TCCTAATGTG
    CCTTTTCATG TGGGAGCAGC AGGACACACT CCTGTGCATG GAATACAAGA GCCTGGAGTG
    TCTCCTCCTA AGCAACAGCT TCCAGTTAAG CCCATCTCCA TGACTTCTGC TTTGAAAGAA
    GTTGGCGTGG ATGGAAATGT AACCGCTGCC CAGGAGGTTA AAGAGGAAAT AAAAAAGCCA
    GATCTTACAA TGCAGAACAA GAGGCAGATA CTGAAAAGAT TGAATCAAAA TCTTAAAGCT
    CAGGAAGATG AGAAAGGAAG AAAGGACGAT AAAAATATCT CTGAGTCAGA TGGGTCTGAA
    GATGGTAAAG AAGAGCAAGA AGATAACCAT CCACTTCTGG GAGATCGCAA AAGATGGGCA
    TCAAGGGCAT CAGATTTGGT AGTTCCTTTA GCTCAGTTAT CAATGGAAGA TTCTCTCTCT
    GGAACAGACC GGCAAACTAT GGGGGAAGTA ATCAGGCTAG ATATTGGGGA ACCCCGCAGG
    AAAGTCTGGG GCAAAAGCCC TACTGATTCG GTCCTGAAGA TTCTAGGAGA AGCGGAGTTG
    CAGCTACCAA CTGAATTACT GGAAGAAATA GATGTAATAA ATGAGCCTGA TGATTTGGAA
    GCAGTGATGT TGAAAGAAAC AGAAGAAGAT AAAAAGCACC AGAGCAAGGA ATTTCCCATT
    GCTGTTAATG AAGTGTGGGT GAAAGAAAAA CAGGATGAGG CACAACAGGA TGCAAGAAAT
    GAAGCAGCTT TTGAGAAAGA AGTAGTGCTT GATGAAGTGC AATCACAAAA TGAAGCTCCA
    GAAGAAACTT GTTCCAGTGA CTCTCTGCAA CCTGAACCGT TATTCCAGAG AGTAATACAG
    CCCCCAGCTG TACCTACAGC CTCACCAGTT CTATCATCTC AGTCTTCCCA AGAAGAGCCT
    TTTGTACCTC GTTCACGTTC CATATCGCCA GCAAAAAATA AAGGGAAAAG TTCATTGTTT
    TTTGGACTTT CTACAGGTCT GTTTGATGCA AACAACCCAA AGATGTTGAG AACGTGTTCA
    CTCCCGGATC TTTACAAACT GTACAGAACC TTAGTTGATG TTCCCAGCAT AGCAGATGTA
    CAGCATCAAC ACAGTCTTGA AATAGATGAT ACGGAAGATG AGCAAGCCAA AGAAGGACCC
    TCTGATTCTG AGGATATCAT GTTTGGGGAC GCCGATACAG ATTTGCAGGA ACTCCGGGCC
    TCAATGGAAC AATTACTTAG GGAGCAGCCT AGTGAGGAAT TAAGTGAAGA GGAGGAATCT
    ACTTTAAAGG CTAACGTCAT CAAATGCATG ACAAATGGTA CAGAGCTGGA TGAAGCAGAT
    GAAAATAACC CCAGCAGTGA AAGTGCACTT AATGAAGAAT GGCAGTCAGA TAATAGTGAT
    GGAGATATTG CCAGTGAATG TGAAGAATGT GATAGTGTCT TCAGCCATTT GGAAGAGCTG
    AGATACAACC TGGAACAGGA AATAGGCTTT GAGAAATTAA TTGAAGTTTA TAATAAAATA
    AAGGCCATAC ATGAAGATGA AGATGAAAAT ATTGATATTT GTTCAAGCAT AGTTCAGACT
    ATTCTGGGAA ATGAACACAA ACACTTATAT GCCAAAGTAC TTCACCTGGT CATGGCGGAT
    GGAGCCTACC AGGAAGATAA TGACGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140867.1
    CDS1006..4575
    Translation

    Target ORF information:

    RefSeq Version XM_015285381.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285381.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
    GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG
    TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT
    CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG
    GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC
    TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT
    GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA
    ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT
    CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC
    AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG
    CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC
    CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA
    AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA
    CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT
    CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT
    TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG
    ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT
    AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG
    TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA
    GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA
    CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT
    GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC
    AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA
    GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG
    AGGCAGAAAG GATGGCTACC TGCAGAAAGA GCAAAGCAAG TTGAAGAATT CTGGCAACGA
    AAGAGGGAAG CCATGCAAAA CAAAGCTCGT GCTGAGGGAC ACATGGGTAC ACTGCAAAAC
    GTGGCAGATA TCTATGGAGG CAGGCCCATG TCTGCAAGAG GAAGGAAATC CAGAAACAAG
    GAAGAAGAGG AATATTTGGC AAGATTAAGA CAGATACGAC TACAAAACTT CAATGAGCGT
    CAACAGATTA AAGCAAAACT TCGTGGAGAG AAGAATGAAG CTCCCAGTTC TGATGGACAA
    GAGTCAAGTG AGGAAGCAGA AATGAAACGC AAAAAGATAG AAGCACAGAA GGCCCAAGCA
    AATGCTCGAG CTGCAGTTCT GAAAGAACAG TTAGAGCGAA AGAGGAAGGA GGCATATGAA
    AGAGAGAAAA GAGCTTGGGA AGAACATCTG ATAGCAAAAG GAGTAAAGAA TCCTAATGTG
    CCTTTTCATG TGGGAGCAGC AGGACACACT CCTGTGCATG GAATACAAGA GCCTGGAGTG
    TCTCCTCCTA AGCAACAGCT TCCAGTTAAG CCCATCTCCA TGACTTCTGC TTTGAAAGAA
    GTTGGCGTGG ATGGAAATGT AACCGCTGCC CAGGAGGTTA AAGAGGAAAT AAAAAAGCCA
    GATCTTACAA TGCAGAACAA GAGGCAGATA CTGAAAAGAT TGAATCAAAA TCTTAAAGCT
    CAGGAAGATG AGAAAGGAAG AAAGGACGAT AAAAATATCT CTGAGTCAGA TGGGTCTGAA
    GATGGTAAAG AAGAGCAAGA AGATAACCAT CCACTTCTGG GAGATCGCAA AAGATGGGCA
    TCAAGGGCAT CAGATTTGGT AGTTCCTTTA GCTCAGTTAT CAATGGAAGA TTCTCTCTCT
    GGAACAGACC GGCAAACTAT GGGGGAAGTA ATCAGGCTAG ATATTGGGGA ACCCCGCAGG
    AAAGTCTGGG GCAAAAGCCC TACTGATTCG GTCCTGAAGA TTCTAGGAGA AGCGGAGTTG
    CAGCTACCAA CTGAATTACT GGAAGAAATA GATGTAATAA ATGAGCCTGA TGATTTGGAA
    GCAGTGATGT TGAAAGAAAC AGAAGAAGAT AAAAAGCACC AGAGCAAGGA ATTTCCCATT
    GCTGTTAATG AAGTGTGGGT GAAAGAAAAA CAGGATGAGG CACAACAGGA TGCAAGAAAT
    GAAGCAGCTT TTGAGAAAGA AGTAGTGCTT GATGAAGTGC AATCACAAAA TGAAGCTCCA
    GAAGAAACTT GTTCCAGTGA CTCTCTGCAA CCTGAACCGT TATTCCAGAG AGTAATACAG
    CCCCCAGCTG TACCTACAGC CTCACCAGTT CTATCATCTC AGTCTTCCCA AGAAGAGCCT
    TTTGTACCTC GTTCACGTTC CATATCGCCA GCAAAAAATA AAGGGAAAAG TTCATTGTTT
    TTTGGACTTT CTACAGGTCT GTTTGATGCA AACAACCCAA AGATGTTGAG AACGTGTTCA
    CTCCCGGATC TTTACAAACT GTACAGAACC TTAGTTGATG TTCCCAGCAT AGCAGATGTA
    CAGCATCAAC ACAGTCTTGA AATAGATGAT ACGGAAGATG AGCAAGCCAA AGAAGGACCC
    TCTGATTCTG AGGATATCAT GTTTGGGGAC GCCGATACAG ATTTGCAGGA ACTCCGGGCC
    TCAATGGAAC AATTACTTAG GGAGCAGCCT AGTGAGGAAT TAAGTGAAGA GGAGGAATCT
    ACTTTAAAGG CTAACGTCAT CAAATGCATG ACAAATGGTA CAGAGCTGGA TGAAGCAGAT
    GAAAATAACC CCAGCAGTGA AAGTGCACTT AATGAAGAAT GGCAGTCAGA TAATAGTGAT
    GGAGATATTG CCAGTGAATG TGAAGAATGT GATAGTGTCT TCAGCCATTT GGAAGAGCTG
    AGATACAACC TGGAACAGGA AATAGGCTTT GAGAAATTAA TTGAAGTTTA TAATAAAATA
    AAGGCCATAC ATGAAGATGA AGATGAAAAT ATTGATATTT GTTCAAGCAT AGTTCAGACT
    ATTCTGGGAA ATGAACACAA ACACTTATAT GCCAAAGTAC TTCACCTGGT CATGGCGGAT
    GGAGCCTACC AGGAAGATAA TGACGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa07770
    Clone ID Related Accession (Same CDS sequence) XM_015285382.1 , XM_015285382.2
    Accession Version XM_015285382.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3486bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase Nek1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455855.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
    GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG
    TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT
    CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG
    GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC
    TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT
    GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA
    ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT
    CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC
    AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG
    CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC
    CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA
    AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA
    CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT
    CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT
    TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG
    ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT
    AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG
    TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA
    GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA
    CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT
    GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC
    AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA
    GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG
    AGGCAGAAAG GATGGCTACC TGCAGAAAGA GCAAAGCAAG TTGAAGAATT CTGGCAACGA
    AAGAGGGAAG CCATGCAAAA CAAAGCTCGT GCTGAGGGAC ACATGGAATA TTTGGCAAGA
    TTAAGACAGA TACGACTACA AAACTTCAAT GAGCGTCAAC AGATTAAAGC AAAACTTCGT
    GGAGAGAAGA ATGAAGCTCC CAGTTCTGAT GGACAAGAGT CAAGTGAGGA AGCAGAAATG
    AAACGCAAAA AGATAGAAGC ACAGAAGGCC CAAGCAAATG CTCGAGCTGC AGTTCTGAAA
    GAACAGTTAG AGCGAAAGAG GAAGGAGGCA TATGAAAGAG AGAAAAGAGC TTGGGAAGAA
    CATCTGATAG CAAAAGGAGT AAAGAATCCT AATGTGCCTT TTCATGTGGG AGCAGCAGGA
    CACACTCCTG TGCATGGAAT ACAAGAGCCT GGAGTGTCTC CTCCTAAGCA ACAGCTTCCA
    GTTAAGCCCA TCTCCATGAC TTCTGCTTTG AAAGAAGTTG GCGTGGATGG AAATGTAACC
    GCTGCCCAGG AGGTTAAAGA GGAAATAAAA AAGCCAGATC TTACAATGCA GAACAAGAGG
    CAGATACTGA AAAGATTGAA TCAAAATCTT AAAGCTCAGG AAGATGAGAA AGGAAGAAAG
    GACGATAAAA ATATCTCTGA GTCAGATGGG TCTGAAGATG GTAAAGAAGA GCAAGAAGAT
    AACCATCCAC TTCTGGGAGA TCGCAAAAGA TGGGCATCAA GGGCATCAGA TTTGGTAGTT
    CCTTTAGCTC AGTTATCAAT GGAAGATTCT CTCTCTGGAA CAGACCGGCA AACTATGGGG
    GAAGTAATCA GGCTAGATAT TGGGGAACCC CGCAGGAAAG TCTGGGGCAA AAGCCCTACT
    GATTCGGTCC TGAAGATTCT AGGAGAAGCG GAGTTGCAGC TACCAACTGA ATTACTGGAA
    GAAATAGATG TAATAAATGA GCCTGATGAT TTGGAAGCAG TGATGTTGAA AGAAACAGAA
    GAAGATAAAA AGCACCAGAG CAAGGAATTT CCCATTGCTG TTAATGAAGT GTGGGTGAAA
    GAAAAACAGG ATGAGGCACA ACAGGATGCA AGAAATGAAG CAGCTTTTGA GAAAGAAGTA
    GTGCTTGATG AAGTGCAATC ACAAAATGAA GCTCCAGAAG AAACTTGTTC CAGTGACTCT
    CTGCAACCTG AACCGTTATT CCAGAGAGTA ATACAGCCCC CAGCTGTACC TACAGCCTCA
    CCAGTTCTAT CATCTCAGTC TTCCCAAGAA GAGCCTTTTG TACCTCGTTC ACGTTCCATA
    TCGCCAGCAA AAAATAAAGG GAAAAGTTCA TTGTTTTTTG GACTTTCTAC AGGTCTGTTT
    GATGCAAACA ACCCAAAGAT GTTGAGAACG TGTTCACTCC CGGATCTTTA CAAACTGTAC
    AGAACCTTAG TTGATGTTCC CAGCATAGCA GATGTACAGC ATCAACACAG TCTTGAAATA
    GATGATACGG AAGATGAGCA AGCCAAAGAA GGACCCTCTG ATTCTGAGGA TATCATGTTT
    GGGGACGCCG ATACAGATTT GCAGGAACTC CGGGCCTCAA TGGAACAATT ACTTAGGGAG
    CAGCCTAGTG AGGAATTAAG TGAAGAGGAG GAATCTACTT TAAAGGCTAA CGTCATCAAA
    TGCATGACAA ATGGTACAGA GCTGGATGAA GCAGATGAAA ATAACCCCAG CAGTGAAAGT
    GCACTTAATG AAGAATGGCA GTCAGATAAT AGTGATGGAG ATATTGCCAG TGAATGTGAA
    GAATGTGATA GTGTCTTCAG CCATTTGGAA GAGCTGAGAT ACAACCTGGA ACAGGAAATA
    GGCTTTGAGA AATTAATTGA AGTTTATAAT AAAATAAAGG CCATACATGA AGATGAAGAT
    GAAAATATTG ATATTTGTTC AAGCATAGTT CAGACTATTC TGGGAAATGA ACACAAACAC
    TTATATGCCA AAGTACTTCA CCTGGTCATG GCGGATGGAG CCTACCAGGA AGATAATGAC
    GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140868.1
    CDS1006..4491
    Translation

    Target ORF information:

    RefSeq Version XM_015285382.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus NIMA-related kinase 1 (NEK1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285382.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
    GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG
    TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT
    CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG
    GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC
    TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT
    GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA
    ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT
    CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC
    AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG
    CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC
    CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA
    AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA
    CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT
    CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT
    TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG
    ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT
    AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG
    TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA
    GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA
    CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT
    GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC
    AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA
    GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG
    AGGCAGAAAG GATGGCTACC TGCAGAAAGA GCAAAGCAAG TTGAAGAATT CTGGCAACGA
    AAGAGGGAAG CCATGCAAAA CAAAGCTCGT GCTGAGGGAC ACATGGAATA TTTGGCAAGA
    TTAAGACAGA TACGACTACA AAACTTCAAT GAGCGTCAAC AGATTAAAGC AAAACTTCGT
    GGAGAGAAGA ATGAAGCTCC CAGTTCTGAT GGACAAGAGT CAAGTGAGGA AGCAGAAATG
    AAACGCAAAA AGATAGAAGC ACAGAAGGCC CAAGCAAATG CTCGAGCTGC AGTTCTGAAA
    GAACAGTTAG AGCGAAAGAG GAAGGAGGCA TATGAAAGAG AGAAAAGAGC TTGGGAAGAA
    CATCTGATAG CAAAAGGAGT AAAGAATCCT AATGTGCCTT TTCATGTGGG AGCAGCAGGA
    CACACTCCTG TGCATGGAAT ACAAGAGCCT GGAGTGTCTC CTCCTAAGCA ACAGCTTCCA
    GTTAAGCCCA TCTCCATGAC TTCTGCTTTG AAAGAAGTTG GCGTGGATGG AAATGTAACC
    GCTGCCCAGG AGGTTAAAGA GGAAATAAAA AAGCCAGATC TTACAATGCA GAACAAGAGG
    CAGATACTGA AAAGATTGAA TCAAAATCTT AAAGCTCAGG AAGATGAGAA AGGAAGAAAG
    GACGATAAAA ATATCTCTGA GTCAGATGGG TCTGAAGATG GTAAAGAAGA GCAAGAAGAT
    AACCATCCAC TTCTGGGAGA TCGCAAAAGA TGGGCATCAA GGGCATCAGA TTTGGTAGTT
    CCTTTAGCTC AGTTATCAAT GGAAGATTCT CTCTCTGGAA CAGACCGGCA AACTATGGGG
    GAAGTAATCA GGCTAGATAT TGGGGAACCC CGCAGGAAAG TCTGGGGCAA AAGCCCTACT
    GATTCGGTCC TGAAGATTCT AGGAGAAGCG GAGTTGCAGC TACCAACTGA ATTACTGGAA
    GAAATAGATG TAATAAATGA GCCTGATGAT TTGGAAGCAG TGATGTTGAA AGAAACAGAA
    GAAGATAAAA AGCACCAGAG CAAGGAATTT CCCATTGCTG TTAATGAAGT GTGGGTGAAA
    GAAAAACAGG ATGAGGCACA ACAGGATGCA AGAAATGAAG CAGCTTTTGA GAAAGAAGTA
    GTGCTTGATG AAGTGCAATC ACAAAATGAA GCTCCAGAAG AAACTTGTTC CAGTGACTCT
    CTGCAACCTG AACCGTTATT CCAGAGAGTA ATACAGCCCC CAGCTGTACC TACAGCCTCA
    CCAGTTCTAT CATCTCAGTC TTCCCAAGAA GAGCCTTTTG TACCTCGTTC ACGTTCCATA
    TCGCCAGCAA AAAATAAAGG GAAAAGTTCA TTGTTTTTTG GACTTTCTAC AGGTCTGTTT
    GATGCAAACA ACCCAAAGAT GTTGAGAACG TGTTCACTCC CGGATCTTTA CAAACTGTAC
    AGAACCTTAG TTGATGTTCC CAGCATAGCA GATGTACAGC ATCAACACAG TCTTGAAATA
    GATGATACGG AAGATGAGCA AGCCAAAGAA GGACCCTCTG ATTCTGAGGA TATCATGTTT
    GGGGACGCCG ATACAGATTT GCAGGAACTC CGGGCCTCAA TGGAACAATT ACTTAGGGAG
    CAGCCTAGTG AGGAATTAAG TGAAGAGGAG GAATCTACTT TAAAGGCTAA CGTCATCAAA
    TGCATGACAA ATGGTACAGA GCTGGATGAA GCAGATGAAA ATAACCCCAG CAGTGAAAGT
    GCACTTAATG AAGAATGGCA GTCAGATAAT AGTGATGGAG ATATTGCCAG TGAATGTGAA
    GAATGTGATA GTGTCTTCAG CCATTTGGAA GAGCTGAGAT ACAACCTGGA ACAGGAAATA
    GGCTTTGAGA AATTAATTGA AGTTTATAAT AAAATAAAGG CCATACATGA AGATGAAGAT
    GAAAATATTG ATATTTGTTC AAGCATAGTT CAGACTATTC TGGGAAATGA ACACAAACAC
    TTATATGCCA AAGTACTTCA CCTGGTCATG GCGGATGGAG CCTACCAGGA AGATAATGAC
    GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa07770
    Clone ID Related Accession (Same CDS sequence) XM_015285382.1 , XM_015285382.2
    Accession Version XM_015285382.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3486bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase Nek1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285382.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
    GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG
    TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT
    CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG
    GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC
    TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT
    GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA
    ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT
    CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC
    AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG
    CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC
    CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA
    AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA
    CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT
    CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT
    TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG
    ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT
    AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG
    TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA
    GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA
    CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT
    GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC
    AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA
    GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG
    AGGCAGAAAG GATGGCTACC TGCAGAAAGA GCAAAGCAAG TTGAAGAATT CTGGCAACGA
    AAGAGGGAAG CCATGCAAAA CAAAGCTCGT GCTGAGGGAC ACATGGAATA TTTGGCAAGA
    TTAAGACAGA TACGACTACA AAACTTCAAT GAGCGTCAAC AGATTAAAGC AAAACTTCGT
    GGAGAGAAGA ATGAAGCTCC CAGTTCTGAT GGACAAGAGT CAAGTGAGGA AGCAGAAATG
    AAACGCAAAA AGATAGAAGC ACAGAAGGCC CAAGCAAATG CTCGAGCTGC AGTTCTGAAA
    GAACAGTTAG AGCGAAAGAG GAAGGAGGCA TATGAAAGAG AGAAAAGAGC TTGGGAAGAA
    CATCTGATAG CAAAAGGAGT AAAGAATCCT AATGTGCCTT TTCATGTGGG AGCAGCAGGA
    CACACTCCTG TGCATGGAAT ACAAGAGCCT GGAGTGTCTC CTCCTAAGCA ACAGCTTCCA
    GTTAAGCCCA TCTCCATGAC TTCTGCTTTG AAAGAAGTTG GCGTGGATGG AAATGTAACC
    GCTGCCCAGG AGGTTAAAGA GGAAATAAAA AAGCCAGATC TTACAATGCA GAACAAGAGG
    CAGATACTGA AAAGATTGAA TCAAAATCTT AAAGCTCAGG AAGATGAGAA AGGAAGAAAG
    GACGATAAAA ATATCTCTGA GTCAGATGGG TCTGAAGATG GTAAAGAAGA GCAAGAAGAT
    AACCATCCAC TTCTGGGAGA TCGCAAAAGA TGGGCATCAA GGGCATCAGA TTTGGTAGTT
    CCTTTAGCTC AGTTATCAAT GGAAGATTCT CTCTCTGGAA CAGACCGGCA AACTATGGGG
    GAAGTAATCA GGCTAGATAT TGGGGAACCC CGCAGGAAAG TCTGGGGCAA AAGCCCTACT
    GATTCGGTCC TGAAGATTCT AGGAGAAGCG GAGTTGCAGC TACCAACTGA ATTACTGGAA
    GAAATAGATG TAATAAATGA GCCTGATGAT TTGGAAGCAG TGATGTTGAA AGAAACAGAA
    GAAGATAAAA AGCACCAGAG CAAGGAATTT CCCATTGCTG TTAATGAAGT GTGGGTGAAA
    GAAAAACAGG ATGAGGCACA ACAGGATGCA AGAAATGAAG CAGCTTTTGA GAAAGAAGTA
    GTGCTTGATG AAGTGCAATC ACAAAATGAA GCTCCAGAAG AAACTTGTTC CAGTGACTCT
    CTGCAACCTG AACCGTTATT CCAGAGAGTA ATACAGCCCC CAGCTGTACC TACAGCCTCA
    CCAGTTCTAT CATCTCAGTC TTCCCAAGAA GAGCCTTTTG TACCTCGTTC ACGTTCCATA
    TCGCCAGCAA AAAATAAAGG GAAAAGTTCA TTGTTTTTTG GACTTTCTAC AGGTCTGTTT
    GATGCAAACA ACCCAAAGAT GTTGAGAACG TGTTCACTCC CGGATCTTTA CAAACTGTAC
    AGAACCTTAG TTGATGTTCC CAGCATAGCA GATGTACAGC ATCAACACAG TCTTGAAATA
    GATGATACGG AAGATGAGCA AGCCAAAGAA GGACCCTCTG ATTCTGAGGA TATCATGTTT
    GGGGACGCCG ATACAGATTT GCAGGAACTC CGGGCCTCAA TGGAACAATT ACTTAGGGAG
    CAGCCTAGTG AGGAATTAAG TGAAGAGGAG GAATCTACTT TAAAGGCTAA CGTCATCAAA
    TGCATGACAA ATGGTACAGA GCTGGATGAA GCAGATGAAA ATAACCCCAG CAGTGAAAGT
    GCACTTAATG AAGAATGGCA GTCAGATAAT AGTGATGGAG ATATTGCCAG TGAATGTGAA
    GAATGTGATA GTGTCTTCAG CCATTTGGAA GAGCTGAGAT ACAACCTGGA ACAGGAAATA
    GGCTTTGAGA AATTAATTGA AGTTTATAAT AAAATAAAGG CCATACATGA AGATGAAGAT
    GAAAATATTG ATATTTGTTC AAGCATAGTT CAGACTATTC TGGGAAATGA ACACAAACAC
    TTATATGCCA AAGTACTTCA CCTGGTCATG GCGGATGGAG CCTACCAGGA AGATAATGAC
    GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140868.1
    CDS1003..4488
    Translation

    Target ORF information:

    RefSeq Version XM_015285382.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus NIMA related kinase 1 (NEK1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285382.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGATAAAT ACATTAAAGT GCAAAAAATT GGAGAAGGAT CCTTTGGGAA AGCATTTCTT 
    GTTAAAGATA AAGAAAATAG CCAGCAGTAT GTTATTAAAG AAATTAACAT ATCTAAGATG
    TCAAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TACTGGCCAA CATGAAACAT
    CCAAATATTG TCCTGTATAG GGAGTCGTTT GAAGAAAATG GTTGTCTCTA CATAGTGATG
    GATTACTGTG AAGGAGGAGA TCTGTTCAAA AAAATAAATG CCCAGAAAGG AGTCCTGTTC
    TCTGAGGATC AGATTCTTGA TTGGTTTGTA CAGATCTGTT TGGCACTGAA ACATATACAT
    GATAGAAAAA TCTTACATCG CGACATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA
    ACAATACAGC TGGGAGATTT TGGAATCGCG AGAGTTCTGA ACAGTACTGT GGAATTGGCT
    CGCACTTGCA TAGGAACACC ATACTATTTA TCACCTGAAA TATGCCAGAA CAGGCCTTAC
    AACAATAAAA GTGATATCTG GGCCCTTGGT TGCGTTCTCT ATGAAATGTG CACACTTAAG
    CATGCATTTG AAGCTGGTAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGATCTTTC
    CCTCCTGTTT CTGTGCATTA TTCCTATGAC CTACGTAATC TACTGTCACA GTTATTTAAA
    AGGAATCCTA GGGATAGACC ATCAGTGAAC TCCATATTGG AGAAAAATTT CATAGCCAAA
    CGGGTTGAAA AATTTCTCAC ACCGCAGCTT ATTGCAGAAG AATTCAGTCA CAAAGTGTTT
    CACAAGTTTG GACCACATGC TGGACCAGCT AAAAGACCAG CTCAAGGACA TATCTCGGCT
    TCAATTGCAC CAGTGCAGAA AATTACCAAA CCTGCTGCAA AATACGGAGT GCCTTTAGCG
    ATGAAGAAGT GTTATGACGC ACCCAAAAAG CTTCATGAGA AAAAGTCATT GATTAAACTT
    AGGCAGGCCT TTCCAACTCC AAAGAAGAAA ATTATTCCAG GAGAAGAAAG GAGGAAAATG
    TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA
    GATCAGATCA GTTTACTGAA GGCAGAGGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA
    CGAATAAACA GAGCCAGGGA GCAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT
    GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGCCTCTTTC
    AGAGGACAGT ATGAGCACTA TCATGCTATT TTTGACCAGA TGCAAAAGGA AACCTTCAAA
    GTAACTAAAG AGAGGCAAGC CAAATGGAGG GCGGAGGTAC AAGGCCAAGA AGCTGTTGAG
    AGGCAGAAAG GATGGCTACC TGCAGAAAGA GCAAAGCAAG TTGAAGAATT CTGGCAACGA
    AAGAGGGAAG CCATGCAAAA CAAAGCTCGT GCTGAGGGAC ACATGGAATA TTTGGCAAGA
    TTAAGACAGA TACGACTACA AAACTTCAAT GAGCGTCAAC AGATTAAAGC AAAACTTCGT
    GGAGAGAAGA ATGAAGCTCC CAGTTCTGAT GGACAAGAGT CAAGTGAGGA AGCAGAAATG
    AAACGCAAAA AGATAGAAGC ACAGAAGGCC CAAGCAAATG CTCGAGCTGC AGTTCTGAAA
    GAACAGTTAG AGCGAAAGAG GAAGGAGGCA TATGAAAGAG AGAAAAGAGC TTGGGAAGAA
    CATCTGATAG CAAAAGGAGT AAAGAATCCT AATGTGCCTT TTCATGTGGG AGCAGCAGGA
    CACACTCCTG TGCATGGAAT ACAAGAGCCT GGAGTGTCTC CTCCTAAGCA ACAGCTTCCA
    GTTAAGCCCA TCTCCATGAC TTCTGCTTTG AAAGAAGTTG GCGTGGATGG AAATGTAACC
    GCTGCCCAGG AGGTTAAAGA GGAAATAAAA AAGCCAGATC TTACAATGCA GAACAAGAGG
    CAGATACTGA AAAGATTGAA TCAAAATCTT AAAGCTCAGG AAGATGAGAA AGGAAGAAAG
    GACGATAAAA ATATCTCTGA GTCAGATGGG TCTGAAGATG GTAAAGAAGA GCAAGAAGAT
    AACCATCCAC TTCTGGGAGA TCGCAAAAGA TGGGCATCAA GGGCATCAGA TTTGGTAGTT
    CCTTTAGCTC AGTTATCAAT GGAAGATTCT CTCTCTGGAA CAGACCGGCA AACTATGGGG
    GAAGTAATCA GGCTAGATAT TGGGGAACCC CGCAGGAAAG TCTGGGGCAA AAGCCCTACT
    GATTCGGTCC TGAAGATTCT AGGAGAAGCG GAGTTGCAGC TACCAACTGA ATTACTGGAA
    GAAATAGATG TAATAAATGA GCCTGATGAT TTGGAAGCAG TGATGTTGAA AGAAACAGAA
    GAAGATAAAA AGCACCAGAG CAAGGAATTT CCCATTGCTG TTAATGAAGT GTGGGTGAAA
    GAAAAACAGG ATGAGGCACA ACAGGATGCA AGAAATGAAG CAGCTTTTGA GAAAGAAGTA
    GTGCTTGATG AAGTGCAATC ACAAAATGAA GCTCCAGAAG AAACTTGTTC CAGTGACTCT
    CTGCAACCTG AACCGTTATT CCAGAGAGTA ATACAGCCCC CAGCTGTACC TACAGCCTCA
    CCAGTTCTAT CATCTCAGTC TTCCCAAGAA GAGCCTTTTG TACCTCGTTC ACGTTCCATA
    TCGCCAGCAA AAAATAAAGG GAAAAGTTCA TTGTTTTTTG GACTTTCTAC AGGTCTGTTT
    GATGCAAACA ACCCAAAGAT GTTGAGAACG TGTTCACTCC CGGATCTTTA CAAACTGTAC
    AGAACCTTAG TTGATGTTCC CAGCATAGCA GATGTACAGC ATCAACACAG TCTTGAAATA
    GATGATACGG AAGATGAGCA AGCCAAAGAA GGACCCTCTG ATTCTGAGGA TATCATGTTT
    GGGGACGCCG ATACAGATTT GCAGGAACTC CGGGCCTCAA TGGAACAATT ACTTAGGGAG
    CAGCCTAGTG AGGAATTAAG TGAAGAGGAG GAATCTACTT TAAAGGCTAA CGTCATCAAA
    TGCATGACAA ATGGTACAGA GCTGGATGAA GCAGATGAAA ATAACCCCAG CAGTGAAAGT
    GCACTTAATG AAGAATGGCA GTCAGATAAT AGTGATGGAG ATATTGCCAG TGAATGTGAA
    GAATGTGATA GTGTCTTCAG CCATTTGGAA GAGCTGAGAT ACAACCTGGA ACAGGAAATA
    GGCTTTGAGA AATTAATTGA AGTTTATAAT AAAATAAAGG CCATACATGA AGATGAAGAT
    GAAAATATTG ATATTTGTTC AAGCATAGTT CAGACTATTC TGGGAAATGA ACACAAACAC
    TTATATGCCA AAGTACTTCA CCTGGTCATG GCGGATGGAG CCTACCAGGA AGATAATGAC
    GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.