NHSL1 gene cDNA ORF clone, Gallus gallus(chicken)

mRNA	Protein	Name
XM_004935685.2	XP_004935742.1	NHS-like protein 1 isoform X5
XM_004935690.3	XP_004935747.1	NHS-like protein 1 isoform X7
XM_419717.6	XP_419717.3	NHS-like protein 1 isoform X8
XM_004935693.3	XP_004935750.1	NHS-like protein 1 isoform X9
XM_004935690.2	XP_004935747.1	NHS-like protein 1 isoform X7
XM_015284194.1	XP_015139680.1	NHS-like protein 1 isoform X4
XM_015284195.1	XP_015139681.1	NHS-like protein 1 isoform X10
XM_015284192.1	XP_015139678.1	NHS-like protein 1 isoform X2
XM_004935687.2	XP_004935744.1	NHS-like protein 1 isoform X6
XM_419717.5	XP_419717.3	NHS-like protein 1 isoform X8
XM_004935693.2	XP_004935750.1	NHS-like protein 1 isoform X9
XM_015284194.2	XP_015139680.1	NHS-like protein 1 isoform X4
XM_004935685.3	XP_004935742.1	NHS-like protein 1 isoform X5
XM_004935689.2	XP_004935746.2	NHS-like protein 1 isoform X1
XM_025149170.1	XP_025004938.1	NHS-like protein 1 isoform X1
XM_025149171.1	XP_025004939.1	NHS-like protein 1 isoform X1
XM_015284193.2	XP_015139679.1	NHS-like protein 1 isoform X3
XM_004935687.3	XP_004935744.1	NHS-like protein 1 isoform X6
XM_015284193.1	XP_015139679.1	NHS-like protein 1 isoform X3
XM_015284192.2	XP_015139678.1	NHS-like protein 1 isoform X2
XM_025149172.1	XP_025004940.1	NHS-like protein 1 isoform X5
XM_015284195.2	XP_015139681.1	NHS-like protein 1 isoform X10

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa06402	XM_004935685.3 Latest version!	Gallus gallus NHS like 1 (NHSL1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OGa06402	XM_025149172.1 Latest version!	Gallus gallus NHS like 1 (NHSL1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OGa06404	XM_004935687.3 Latest version!	Gallus gallus NHS like 1 (NHSL1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OGa06407	XM_004935690.3 Latest version!	Gallus gallus NHS like 1 (NHSL1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OGa06410	XM_419717.6 Latest version!	Gallus gallus NHS like 1 (NHSL1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OGa06411	XM_004935693.3 Latest version!	Gallus gallus NHS like 1 (NHSL1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OGa33681	XM_025149170.1 Latest version!	Gallus gallus NHS like 1 (NHSL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OGa33681	XM_025149171.1 Latest version!	Gallus gallus NHS like 1 (NHSL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OGa33681	XM_004935689.2 Latest version!	Gallus gallus NHS-like 1 (NHSL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OGa33682	XM_015284192.2 Latest version!	Gallus gallus NHS like 1 (NHSL1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OGa33683	XM_015284193.2 Latest version!	Gallus gallus NHS like 1 (NHSL1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OGa33684	XM_015284194.2 Latest version!	Gallus gallus NHS like 1 (NHSL1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OGa33685	XM_015284195.2 Latest version!	Gallus gallus NHS like 1 (NHSL1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
View Old Accession Versions >>
OGa06407	XM_004935690.2	Gallus gallus NHS-like 1 (NHSL1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OGa06410	XM_419717.5	Gallus gallus NHS-like 1 (NHSL1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OGa06411	XM_004935693.2	Gallus gallus NHS-like 1 (NHSL1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OGa33684	XM_015284194.1	Gallus gallus NHS-like 1 (NHSL1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OGa06402	XM_004935685.2	Gallus gallus NHS-like 1 (NHSL1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OGa06404	XM_004935687.2	Gallus gallus NHS-like 1 (NHSL1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OGa33683	XM_015284193.1	Gallus gallus NHS-like 1 (NHSL1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OGa33682	XM_015284192.1	Gallus gallus NHS-like 1 (NHSL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OGa33685	XM_015284195.1	Gallus gallus NHS-like 1 (NHSL1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
Hide Old Accession Versions >>

NHSL1 ( XM_004935685.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004935742.1 Gallus gallus NHS-like 1 (NHSL1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OGa06402
Clone ID Related Accession (Same CDS sequence)	XM_004935685.2 , XM_004935685.3 , XM_025149172.1
Accession Version	XM_004935685.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	NHS-like protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455837.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004935685.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAGG AGTGTGTTTC GAGATCTTTC AAACTCAAAC AAAACGCAGG CTCCCTCTCG 
CGTGCTGTAA GCTGGATAAA TTTCTCCTCC TTGTCAAGGC AGACAAAGAG ATTGTTTCGC 
AGCGATGGTG AGCTCTCCTC CATTTGCGTG CAGCAGGTAG ACGAGGATGA TGAGAACTGG 
ACCTACAGGA GCCCACGCGG AAAAGCTGTT TCCAACTTAG ATGAAGAGAG CAGATGGACC 
GTCCATTACA CTGCTCCATG GCACCAGCAG GAAAATGTGT TCCTGCCTTC CTCAAGGCCG 
CCTTGTGTGG AGGACTTGCA CCGGCAAGCA AAGCTGAACC TCAAGTCAGT ACTGAGAGAG 
TGTGACAAGC TGCGGAGAGA TGGCTATCGG AGCTCTCAGT ATTACTCTCA GGGCCCCACC 
TTCTCCTCAT CCTCCAGTGC AATCTGTGGA AGTTACCAGG ACGATTATGA AGAAATTGAA 
CAAAAGTGTT CTGTCTCTTC CCCAGAAGAA GAAAAGCTTA TCACCATCAA AAGGCCTAAA 
ACCCCAGTTT CAAATGACTT ATCAGATATC AACACACAAA CCAACTGGAC TAAGTCACTT 
CCACTGCCAA CACCAGAAGA GAAGATGCGA CAGCAAGCAC AGGCAGTCCA AACAGATGTG 
GTTCCTATCA ATGTGACTGG GGAGAATTTC GACCGCCAAG CCAGCATTCG GCGGTCTCTA 
ATTTACACAG ACACGGTGGT AAGACGGCCG AAGAAAGTCA AAAGGAGAAA GACTATAACA 
GGAATCCCTG ACAACATACA AAAGGAGCTA GCAGTTGGCA CAGGCCAAAG TGATTTCCGA 
GGGCACTCGA TGTATGTCCC AGATCACAGT TCCACGCTAG GGCGACTAGA CAGCTATCGC 
TCTGCTATGC AGCGGTCTGA AACCAAGGAC ACCAGCTGCC AGACTGAAGA AGTTAAAGTT 
GTGCCTCCTT CGATGAGAAG AATACGAGCA CAGAAAGGAC AAGGCATTGC AGCTCAGATG 
TCTCAGTTCT CCAGCTCATC TGGAAACATG TCTGTGATGA GTGATTCTGC TGCAGTTATA 
TTTGCTTCTC GCCAGAATAG TGACATAGGT TTTCACAGCT TGCCTCGGGC TGGTGCAAGA 
GTGTCTCTGC AGTCCTTAGA ACAGACCCAG AGCATCTCCA GACAGACAGA AGACATTGCT 
GGTACTTTGC CTCACCAGAT GAGTAAACTG CAAGTGGATG ATGATGTTGC ACATCTGAGA 
AATAATGCCA TAACAGGAAC TGTTTCGAGG CCAAAGTCTC AAGAGGTAAG AAGTTGTGAC 
AGTGAAAAGT CCACTAGCCC AGCATGTGTG GTCTCTCCTC ATGCCACCTA CTCAACAAGC 
GTCATACCCA ATGCGACACT GTCATCCTCC TCAGAAGTTA TTGCTATTCA CACTGCCCAG 
AGTACTGGAT CGCTGGATAA CAAAATTAGC AGTTCTGCTG TGTACCCAAA ACCAAGAGAT 
AATCCTGTTG CCAACAATGC AATAAGTGGG AAAGAAGATC ACCATTCTTC AAGTGGTAAC 
TGGAGTGAGA GTAGCTCCAC ACGTCACTCA CAGACTTCAG ATACCATCCC ATCTCATACT 
GTCATGATGC TTTCTCTTGG TGACTCTGCT GTCTCTCTCA GCACTCCTGG AAATGTAGAG 
GCTGGCTCTC AGAGTGTGAA CTACAGCTGC AGAAGCAATC TTGCTTCACC AAACCAGTCC 
CAGGACAGCG ATGGTAGGAG TGAATCTAGC TACTCAGGGG AGCGAACACA AGGTGTAGTG 
AGCAGCACAG AGCACTGGCT ATACAAGTCT GCGGAAAATA GTGAGACTGC ATCATGCAAG 
GTAGCTTGTA CCACACCAGG CTGTGCCACT CCTGGTAGCA ACCTGAGCAG CAGCAGCCTG 
GAGAGGACAT CAGCCAAAGA AGATTCCACT TCACTGTATT CTGTAGACCA CGATGGTTAT 
TACAGCTCTA TGAGCATGGA CTCTGGACTG AAGTCAGACA TGCCATGCTA TAGTACTAAT 
GGTTTTGGGA ACCCCAGAGA CAGCGTGATT AATGTCTTTG AAGCAAAGGA GAAGAAACAT 
CAAGATGACC AGCCAGGCCA AGGTGAAAAA TCCCTTGCAA GAAACATCTC TCTAAAAAAG 
GCAAAGAAGC CGCCTTTACC TCCGTCTAGA ACAGACTCAC TCAGAAGGGT ACCCAAGAAG 
AAAGCTCAGT CCAATGGACA GGTACTTGAT GAAACCCTCA TTGCTACCCT CCAGCATTCT 
CTGCAGTTGA ATCTCAAGTG CAAAAATGGC AGCTCCCCTT CCCAGAGCCC CTGCAGTGAC 
TATGAGGATC CCTGGGTGTT GCGCTCTCGC AGCCAAAGCT CGGTCAGCGC AAGCAGCAGC 
ATGATGTCCA CTACTGCCCC AAACCTGTAC TCCATTTGCA CAGTCACTCC CTCTCAGAGT 
GAAACAAGCA GCATCAAGTC AGAGTATGCT GACCAGTGGG GTTACTACAG TGACTATGCT 
GCAGTGGCAG ATGACCAGAT GAAGTCTCCA GTGACCCATT CTGCTAGTAC CTCTTCTGCT 
CTCAGTGATT ACAGCATCAG CCACTTCAGT GATGGCTCAA GGGCTTCTGT ACCACAAGTG 
CCCGCTGGAC TGGCCAAACC AAAGAGCACT TCTCCAGAGA AGTCCCACCG AGTCACATCG 
CCATCCAGTG GGTACTCCAG CCAGTCCAAT ACACCCACTG CACTCACTCC AGTGCCTGTG 
TTTTTAAAAT CTGCGTCATC AGGAAATGGG AAATCCAAGC TGAAACCTAA AGTGCCTGAA 
AGGAAATCTT CTCTTCTGTC TTCAGTCTCC ATTTCTTCAT CCTCTACTTC TCTTTCTTCA 
AATACATCTA CTGAAGGGAG TGTGAATGTG AAGAAATTGG ACCCCACCCC GAGTTCTCCT 
CCAGATTCTG GTGCACCTCT TCCTCCGCCT CCTCTTCTAG CACCTCCTCC ACATTGCCTT 
GAGCTCTCTC CTCCCTCTTC CCCTCCTCCT CCTCCAGCAG AAGCCGTGGA TATATCACCA 
TTGCCAGCTT CTCCCACATT CCCCCCCCCT CCACCAGAAG CTGATGTAAA TTCTTCCTTT 
GCTCACAGTG TCCAGTGGTT ACCACAAGAA GCTTCCATCG GTTCGTACTC TTCCCCTGTT 
CCTCCTCCTG TTTTCCCTTT AGTTGTCCCA CCACCAGCCC CACCTCTTGA TCCCAAACTG 
ACAAAAGGTG CAATGATATG CCCACAGTAT TCATTTAAGA AACATGATCA GGAAGATTCT 
TGCTACAATG CTGTGAAACA GCCATTCAGC AAGCAAGATG CATCAAGACC TGTGATGCCC 
TTGGTAACCA CCAAAGCATT GCAAATGGTG CAGCTGAGGT CTATCAGAAA AGCAACAGAA 
GGTGACCCAT CACCTGAATC TGCTTCTGAA ACCATCTCTC AGGAAAAGGG TATTGTCACT 
TCATCATCAC AGTCTTCCCT AAGGCCATCT CTCTCACTAC GACTCAGTAA CAGCTTAGGT 
GAAGAGGAGA TGAAGATTCA TGGCACTTCA TTTAAAAACA CACCTCAAAC ACTGGCTCAG 
AGTTCTCCTC TGGTACTCTC TGACAACGTA CCCATGCTTG AGAGCGATCA AAAAGCTGCA 
GTTACTATGG GTCTAAGCAA GTCTTTGGAA CCAGATTCCC CAGGGTCAGC TCCAGAAGCC 
ACACCCAAGT CCCCAGTTCG GAGCGAAGAC TTCCATTCTG GCATGCCACT GCAGGGGTCA 
CCAGCATCTC CAGACAAGAC TCAGGTCGTT TTGCCAAACA AGAAACCACC TCCCATTTCA 
AAGAAACCCA AACTGTTCCT TGTTGTACCA CCTCCACAGT TAGATCTCAC AGTGGAGAAA 
ATAGCTGAAG TGAGTGATAC TGTCAGAAGC ACATCAAGCC CAACTAAGAG AGACGCTGTG 
CTTACACACT GCGAGGAGGC GAGAAATTGC CTTACAGATG GACTCAGCTC CAGCGAGATG 
GACTCTGGCA GCCTGGTTCC TGAGGGAGGA GCTGCCAGAG TCACCTTCTC TGAAGCAGTG 
GGAGTTGGTG CCTTTGTGGT ACAGTCCGCT GCATCACCAG TTCAAGAGGA GCCCAGGCAA 
GAGGAGCAGT CAGTTTTTGA TGAAGGAAGC AGCTCAGGCA GCAGCCAAGA CAGCAGCAGT 
AACACTGAAG GACACCCGTC TCAGGAGAAC GAAAGTGCCG AGGTGTTTGA ATCAGAAACA 
GCAAATAGTT CATTCCTGCC AAGCAATAAT TATGGGGAAG AGACGGATGG AGTGGCAACA 
CCAGCGAGAC CGAGGACTAC TGAGGATCTT TTTGCAGCCA TTCACAGATC CAAAAGGAAA 
GTCCTTGGCC GTAAGGATTC TGAGGACGAT CGTACCCGCA ACCATTCTCC TTCACCCCCC 
GTAACACCCA CTGGTGCTTC CCCAACCTTA TCTACCCTCA AACAGGCAGG ATCTATTCAG 
AGAAGCGTTC GCAAGAGCAG CACCAGCAAT GACAACTTCA AAGCCCTGCT GCTGAAGAAA 
GGGAGTCGCT GTGACACCAG TTCCCGCATG TCAGCAGCAG AGATGTTGAA GCACACGGAT 
CCACGGTTCC ACCGAGCCAA GGTGGATGCC TCTCCCGACC TTTCCGACAG CCCTGCCAGC 
TGCTCGCCCA GCAAGAGCAA ACGGGCCCAA GAGGAGTGGG CCAAGAGTGA AGGCCTGATG 
CCGAGGAGCA TGTCCCTCTC CAGCACTAGG TATGGCCGAT CACGAACACC GCCCTCCGCT 
GCCAGCAGCA AGTACAATGT TCGCAACCGC ATCCAGAGCA GCCCCATGAC AGTGATTAGC 
GAAGGAGATG GGGAAGTGGT GGAGCAGACA GAGAGCAGGG TCCAAAGGAC TTTGGATGAG 
CAGCGAGAGA GGCAGCTCGA TGTATTTAAC AGTGATGAAA TAGACATGAA TGACTTTTCG 
TATTCTGAGG AGCTGGACTG CAAGGAGACC TTGGATCCAA CCCATCTGGA CTTAATGACT 
CAGCCGGGTA CTTCGAGAAA GTACCTAAGT CCTTCAGCTG AAGAAAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004935742.1
CDS	396..5486
Translation	MKKECVSRSFKLKQNAGSLSRAVSWINFSSLSRQTKRLFRSDGELSSICVQQVDEDDENWTYRSPRGKAVSNLDEESRWTVHYTAPWHQQENVFLPSSRPPCVEDLHRQAKLNLKSVLRECDKLRRDGYRSSQYYSQGPTFSSSSSAICGSYQDDYEEIEQKCSVSSPEEEKLITIKRPKTPVSNDLSDINTQTNWTKSLPLPTPEEKMRQQAQAVQTDVVPINVTGENFDRQASIRRSLIYTDTVVRRPKKVKRRKTITGIPDNIQKELAVGTGQSDFRGHSMYVPDHSSTLGRLDSYRSAMQRSETKDTSCQTEEVKVVPPSMRRIRAQKGQGIAAQMSQFSSSSGNMSVMSDSAAVIFASRQNSDIGFHSLPRAGARVSLQSLEQTQSISRQTEDIAGTLPHQMSKLQVDDDVAHLRNNAITGTVSRPKSQEVRSCDSEKSTSPACVVSPHATYSTSVIPNATLSSSSEVIAIHTAQSTGSLDNKISSSAVYPKPRDNPVANNAISGKEDHHSSSGNWSESSSTRHSQTSDTIPSHTVMMLSLGDSAVSLSTPGNVEAGSQSVNYSCRSNLASPNQSQDSDGRSESSYSGERTQGVVSSTEHWLYKSAENSETASCKVACTTPGCATPGSNLSSSSLERTSAKEDSTSLYSVDHDGYYSSMSMDSGLKSDMPCYSTNGFGNPRDSVINVFEAKEKKHQDDQPGQGEKSLARNISLKKAKKPPLPPSRTDSLRRVPKKKAQSNGQVLDETLIATLQHSLQLNLKCKNGSSPSQSPCSDYEDPWVLRSRSQSSVSASSSMMSTTAPNLYSICTVTPSQSETSSIKSEYADQWGYYSDYAAVADDQMKSPVTHSASTSSALSDYSISHFSDGSRASVPQVPAGLAKPKSTSPEKSHRVTSPSSGYSSQSNTPTALTPVPVFLKSASSGNGKSKLKPKVPERKSSLLSSVSISSSSTSLSSNTSTEGSVNVKKLDPTPSSPPDSGAPLPPPPLLAPPPHCLELSPPSSPPPPPAEAVDISPLPASPTFPPPPPEADVNSSFAHSVQWLPQEASIGSYSSPVPPPVFPLVVPPPAPPLDPKLTKGAMICPQYSFKKHDQEDSCYNAVKQPFSKQDASRPVMPLVTTKALQMVQLRSIRKATEGDPSPESASETISQEKGIVTSSSQSSLRPSLSLRLSNSLGEEEMKIHGTSFKNTPQTLAQSSPLVLSDNVPMLESDQKAAVTMGLSKSLEPDSPGSAPEATPKSPVRSEDFHSGMPLQGSPASPDKTQVVLPNKKPPPISKKPKLFLVVPPPQLDLTVEKIAEVSDTVRSTSSPTKRDAVLTHCEEARNCLTDGLSSSEMDSGSLVPEGGAARVTFSEAVGVGAFVVQSAASPVQEEPRQEEQSVFDEGSSSGSSQDSSSNTEGHPSQENESAEVFESETANSSFLPSNNYGEETDGVATPARPRTTEDLFAAIHRSKRKVLGRKDSEDDRTRNHSPSPPVTPTGASPTLSTLKQAGSIQRSVRKSSTSNDNFKALLLKKGSRCDTSSRMSAAEMLKHTDPRFHRAKVDASPDLSDSPASCSPSKSKRAQEEWAKSEGLMPRSMSLSSTRYGRSRTPPSAASSKYNVRNRIQSSPMTVISEGDGEVVEQTESRVQRTLDEQRERQLDVFNSDEIDMNDFSYSEELDCKETLDPTHLDLMTQPGTSRKYLSPSAEES

Target ORF information:

RefSeq Version	XM_004935685.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NHS-like 1 (NHSL1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004935685.2

ORF Insert Sequence:

ATGAAAAAGG AGTGTGTTTC GAGATCTTTC AAACTCAAAC AAAACGCAGG CTCCCTCTCG 
CGTGCTGTAA GCTGGATAAA TTTCTCCTCC TTGTCAAGGC AGACAAAGAG ATTGTTTCGC 
AGCGATGGTG AGCTCTCCTC CATTTGCGTG CAGCAGGTAG ACGAGGATGA TGAGAACTGG 
ACCTACAGGA GCCCACGCGG AAAAGCTGTT TCCAACTTAG ATGAAGAGAG CAGATGGACC 
GTCCATTACA CTGCTCCATG GCACCAGCAG GAAAATGTGT TCCTGCCTTC CTCAAGGCCG 
CCTTGTGTGG AGGACTTGCA CCGGCAAGCA AAGCTGAACC TCAAGTCAGT ACTGAGAGAG 
TGTGACAAGC TGCGGAGAGA TGGCTATCGG AGCTCTCAGT ATTACTCTCA GGGCCCCACC 
TTCTCCTCAT CCTCCAGTGC AATCTGTGGA AGTTACCAGG ACGATTATGA AGAAATTGAA 
CAAAAGTGTT CTGTCTCTTC CCCAGAAGAA GAAAAGCTTA TCACCATCAA AAGGCCTAAA 
ACCCCAGTTT CAAATGACTT ATCAGATATC AACACACAAA CCAACTGGAC TAAGTCACTT 
CCACTGCCAA CACCAGAAGA GAAGATGCGA CAGCAAGCAC AGGCAGTCCA AACAGATGTG 
GTTCCTATCA ATGTGACTGG GGAGAATTTC GACCGCCAAG CCAGCATTCG GCGGTCTCTA 
ATTTACACAG ACACGGTGGT AAGACGGCCG AAGAAAGTCA AAAGGAGAAA GACTATAACA 
GGAATCCCTG ACAACATACA AAAGGAGCTA GCAGTTGGCA CAGGCCAAAG TGATTTCCGA 
GGGCACTCGA TGTATGTCCC AGATCACAGT TCCACGCTAG GGCGACTAGA CAGCTATCGC 
TCTGCTATGC AGCGGTCTGA AACCAAGGAC ACCAGCTGCC AGACTGAAGA AGTTAAAGTT 
GTGCCTCCTT CGATGAGAAG AATACGAGCA CAGAAAGGAC AAGGCATTGC AGCTCAGATG 
TCTCAGTTCT CCAGCTCATC TGGAAACATG TCTGTGATGA GTGATTCTGC TGCAGTTATA 
TTTGCTTCTC GCCAGAATAG TGACATAGGT TTTCACAGCT TGCCTCGGGC TGGTGCAAGA 
GTGTCTCTGC AGTCCTTAGA ACAGACCCAG AGCATCTCCA GACAGACAGA AGACATTGCT 
GGTACTTTGC CTCACCAGAT GAGTAAACTG CAAGTGGATG ATGATGTTGC ACATCTGAGA 
AATAATGCCA TAACAGGAAC TGTTTCGAGG CCAAAGTCTC AAGAGGTAAG AAGTTGTGAC 
AGTGAAAAGT CCACTAGCCC AGCATGTGTG GTCTCTCCTC ATGCCACCTA CTCAACAAGC 
GTCATACCCA ATGCGACACT GTCATCCTCC TCAGAAGTTA TTGCTATTCA CACTGCCCAG 
AGTACTGGAT CGCTGGATAA CAAAATTAGC AGTTCTGCTG TGTACCCAAA ACCAAGAGAT 
AATCCTGTTG CCAACAATGC AATAAGTGGG AAAGAAGATC ACCATTCTTC AAGTGGTAAC 
TGGAGTGAGA GTAGCTCCAC ACGTCACTCA CAGACTTCAG ATACCATCCC ATCTCATACT 
GTCATGATGC TTTCTCTTGG TGACTCTGCT GTCTCTCTCA GCACTCCTGG AAATGTAGAG 
GCTGGCTCTC AGAGTGTGAA CTACAGCTGC AGAAGCAATC TTGCTTCACC AAACCAGTCC 
CAGGACAGCG ATGGTAGGAG TGAATCTAGC TACTCAGGGG AGCGAACACA AGGTGTAGTG 
AGCAGCACAG AGCACTGGCT ATACAAGTCT GCGGAAAATA GTGAGACTGC ATCATGCAAG 
GTAGCTTGTA CCACACCAGG CTGTGCCACT CCTGGTAGCA ACCTGAGCAG CAGCAGCCTG 
GAGAGGACAT CAGCCAAAGA AGATTCCACT TCACTGTATT CTGTAGACCA CGATGGTTAT 
TACAGCTCTA TGAGCATGGA CTCTGGACTG AAGTCAGACA TGCCATGCTA TAGTACTAAT 
GGTTTTGGGA ACCCCAGAGA CAGCGTGATT AATGTCTTTG AAGCAAAGGA GAAGAAACAT 
CAAGATGACC AGCCAGGCCA AGGTGAAAAA TCCCTTGCAA GAAACATCTC TCTAAAAAAG 
GCAAAGAAGC CGCCTTTACC TCCGTCTAGA ACAGACTCAC TCAGAAGGGT ACCCAAGAAG 
AAAGCTCAGT CCAATGGACA GGTACTTGAT GAAACCCTCA TTGCTACCCT CCAGCATTCT 
CTGCAGTTGA ATCTCAAGTG CAAAAATGGC AGCTCCCCTT CCCAGAGCCC CTGCAGTGAC 
TATGAGGATC CCTGGGTGTT GCGCTCTCGC AGCCAAAGCT CGGTCAGCGC AAGCAGCAGC 
ATGATGTCCA CTACTGCCCC AAACCTGTAC TCCATTTGCA CAGTCACTCC CTCTCAGAGT 
GAAACAAGCA GCATCAAGTC AGAGTATGCT GACCAGTGGG GTTACTACAG TGACTATGCT 
GCAGTGGCAG ATGACCAGAT GAAGTCTCCA GTGACCCATT CTGCTAGTAC CTCTTCTGCT 
CTCAGTGATT ACAGCATCAG CCACTTCAGT GATGGCTCAA GGGCTTCTGT ACCACAAGTG 
CCCGCTGGAC TGGCCAAACC AAAGAGCACT TCTCCAGAGA AGTCCCACCG AGTCACATCG 
CCATCCAGTG GGTACTCCAG CCAGTCCAAT ACACCCACTG CACTCACTCC AGTGCCTGTG 
TTTTTAAAAT CTGCGTCATC AGGAAATGGG AAATCCAAGC TGAAACCTAA AGTGCCTGAA 
AGGAAATCTT CTCTTCTGTC TTCAGTCTCC ATTTCTTCAT CCTCTACTTC TCTTTCTTCA 
AATACATCTA CTGAAGGGAG TGTGAATGTG AAGAAATTGG ACCCCACCCC GAGTTCTCCT 
CCAGATTCTG GTGCACCTCT TCCTCCGCCT CCTCTTCTAG CACCTCCTCC ACATTGCCTT 
GAGCTCTCTC CTCCCTCTTC CCCTCCTCCT CCTCCAGCAG AAGCCGTGGA TATATCACCA 
TTGCCAGCTT CTCCCACATT CCCCCCCCCT CCACCAGAAG CTGATGTAAA TTCTTCCTTT 
GCTCACAGTG TCCAGTGGTT ACCACAAGAA GCTTCCATCG GTTCGTACTC TTCCCCTGTT 
CCTCCTCCTG TTTTCCCTTT AGTTGTCCCA CCACCAGCCC CACCTCTTGA TCCCAAACTG 
ACAAAAGGTG CAATGATATG CCCACAGTAT TCATTTAAGA AACATGATCA GGAAGATTCT 
TGCTACAATG CTGTGAAACA GCCATTCAGC AAGCAAGATG CATCAAGACC TGTGATGCCC 
TTGGTAACCA CCAAAGCATT GCAAATGGTG CAGCTGAGGT CTATCAGAAA AGCAACAGAA 
GGTGACCCAT CACCTGAATC TGCTTCTGAA ACCATCTCTC AGGAAAAGGG TATTGTCACT 
TCATCATCAC AGTCTTCCCT AAGGCCATCT CTCTCACTAC GACTCAGTAA CAGCTTAGGT 
GAAGAGGAGA TGAAGATTCA TGGCACTTCA TTTAAAAACA CACCTCAAAC ACTGGCTCAG 
AGTTCTCCTC TGGTACTCTC TGACAACGTA CCCATGCTTG AGAGCGATCA AAAAGCTGCA 
GTTACTATGG GTCTAAGCAA GTCTTTGGAA CCAGATTCCC CAGGGTCAGC TCCAGAAGCC 
ACACCCAAGT CCCCAGTTCG GAGCGAAGAC TTCCATTCTG GCATGCCACT GCAGGGGTCA 
CCAGCATCTC CAGACAAGAC TCAGGTCGTT TTGCCAAACA AGAAACCACC TCCCATTTCA 
AAGAAACCCA AACTGTTCCT TGTTGTACCA CCTCCACAGT TAGATCTCAC AGTGGAGAAA 
ATAGCTGAAG TGAGTGATAC TGTCAGAAGC ACATCAAGCC CAACTAAGAG AGACGCTGTG 
CTTACACACT GCGAGGAGGC GAGAAATTGC CTTACAGATG GACTCAGCTC CAGCGAGATG 
GACTCTGGCA GCCTGGTTCC TGAGGGAGGA GCTGCCAGAG TCACCTTCTC TGAAGCAGTG 
GGAGTTGGTG CCTTTGTGGT ACAGTCCGCT GCATCACCAG TTCAAGAGGA GCCCAGGCAA 
GAGGAGCAGT CAGTTTTTGA TGAAGGAAGC AGCTCAGGCA GCAGCCAAGA CAGCAGCAGT 
AACACTGAAG GACACCCGTC TCAGGAGAAC GAAAGTGCCG AGGTGTTTGA ATCAGAAACA 
GCAAATAGTT CATTCCTGCC AAGCAATAAT TATGGGGAAG AGACGGATGG AGTGGCAACA 
CCAGCGAGAC CGAGGACTAC TGAGGATCTT TTTGCAGCCA TTCACAGATC CAAAAGGAAA 
GTCCTTGGCC GTAAGGATTC TGAGGACGAT CGTACCCGCA ACCATTCTCC TTCACCCCCC 
GTAACACCCA CTGGTGCTTC CCCAACCTTA TCTACCCTCA AACAGGCAGG ATCTATTCAG 
AGAAGCGTTC GCAAGAGCAG CACCAGCAAT GACAACTTCA AAGCCCTGCT GCTGAAGAAA 
GGGAGTCGCT GTGACACCAG TTCCCGCATG TCAGCAGCAG AGATGTTGAA GCACACGGAT 
CCACGGTTCC ACCGAGCCAA GGTGGATGCC TCTCCCGACC TTTCCGACAG CCCTGCCAGC 
TGCTCGCCCA GCAAGAGCAA ACGGGCCCAA GAGGAGTGGG CCAAGAGTGA AGGCCTGATG 
CCGAGGAGCA TGTCCCTCTC CAGCACTAGG TATGGCCGAT CACGAACACC GCCCTCCGCT 
GCCAGCAGCA AGTACAATGT TCGCAACCGC ATCCAGAGCA GCCCCATGAC AGTGATTAGC 
GAAGGAGATG GGGAAGTGGT GGAGCAGACA GAGAGCAGGG TCCAAAGGAC TTTGGATGAG 
CAGCGAGAGA GGCAGCTCGA TGTATTTAAC AGTGATGAAA TAGACATGAA TGACTTTTCG 
TATTCTGAGG AGCTGGACTG CAAGGAGACC TTGGATCCAA CCCATCTGGA CTTAATGACT 
CAGCCGGGTA CTTCGAGAAA GTACCTAAGT CCTTCAGCTG AAGAAAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_004935685.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004935742.1 Gallus gallus NHS like 1 (NHSL1), transcript variant X7, mRNA.

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CloneID	OGa06402
Clone ID Related Accession (Same CDS sequence)	XM_004935685.2 , XM_004935685.3 , XM_025149172.1
Accession Version	XM_004935685.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	NHS-like protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004935685.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAGG AGTGTGTTTC GAGATCTTTC AAACTCAAAC AAAACGCAGG CTCCCTCTCG 
CGTGCTGTAA GCTGGATAAA TTTCTCCTCC TTGTCAAGGC AGACAAAGAG ATTGTTTCGC 
AGCGATGGTG AGCTCTCCTC CATTTGCGTG CAGCAGGTAG ACGAGGATGA TGAGAACTGG 
ACCTACAGGA GCCCACGCGG AAAAGCTGTT TCCAACTTAG ATGAAGAGAG CAGATGGACC 
GTCCATTACA CTGCTCCATG GCACCAGCAG GAAAATGTGT TCCTGCCTTC CTCAAGGCCG 
CCTTGTGTGG AGGACTTGCA CCGGCAAGCA AAGCTGAACC TCAAGTCAGT ACTGAGAGAG 
TGTGACAAGC TGCGGAGAGA TGGCTATCGG AGCTCTCAGT ATTACTCTCA GGGCCCCACC 
TTCTCCTCAT CCTCCAGTGC AATCTGTGGA AGTTACCAGG ACGATTATGA AGAAATTGAA 
CAAAAGTGTT CTGTCTCTTC CCCAGAAGAA GAAAAGCTTA TCACCATCAA AAGGCCTAAA 
ACCCCAGTTT CAAATGACTT ATCAGATATC AACACACAAA CCAACTGGAC TAAGTCACTT 
CCACTGCCAA CACCAGAAGA GAAGATGCGA CAGCAAGCAC AGGCAGTCCA AACAGATGTG 
GTTCCTATCA ATGTGACTGG GGAGAATTTC GACCGCCAAG CCAGCATTCG GCGGTCTCTA 
ATTTACACAG ACACGGTGGT AAGACGGCCG AAGAAAGTCA AAAGGAGAAA GACTATAACA 
GGAATCCCTG ACAACATACA AAAGGAGCTA GCAGTTGGCA CAGGCCAAAG TGATTTCCGA 
GGGCACTCGA TGTATGTCCC AGATCACAGT TCCACGCTAG GGCGACTAGA CAGCTATCGC 
TCTGCTATGC AGCGGTCTGA AACCAAGGAC ACCAGCTGCC AGACTGAAGA AGTTAAAGTT 
GTGCCTCCTT CGATGAGAAG AATACGAGCA CAGAAAGGAC AAGGCATTGC AGCTCAGATG 
TCTCAGTTCT CCAGCTCATC TGGAAACATG TCTGTGATGA GTGATTCTGC TGCAGTTATA 
TTTGCTTCTC GCCAGAATAG TGACATAGGT TTTCACAGCT TGCCTCGGGC TGGTGCAAGA 
GTGTCTCTGC AGTCCTTAGA ACAGACCCAG AGCATCTCCA GACAGACAGA AGACATTGCT 
GGTACTTTGC CTCACCAGAT GAGTAAACTG CAAGTGGATG ATGATGTTGC ACATCTGAGA 
AATAATGCCA TAACAGGAAC TGTTTCGAGG CCAAAGTCTC AAGAGGTAAG AAGTTGTGAC 
AGTGAAAAGT CCACTAGCCC AGCATGTGTG GTCTCTCCTC ATGCCACCTA CTCAACAAGC 
GTCATACCCA ATGCGACACT GTCATCCTCC TCAGAAGTTA TTGCTATTCA CACTGCCCAG 
AGTACTGGAT CGCTGGATAA CAAAATTAGC AGTTCTGCTG TGTACCCAAA ACCAAGAGAT 
AATCCTGTTG CCAACAATGC AATAAGTGGG AAAGAAGATC ACCATTCTTC AAGTGGTAAC 
TGGAGTGAGA GTAGCTCCAC ACGTCACTCA CAGACTTCAG ATACCATCCC ATCTCATACT 
GTCATGATGC TTTCTCTTGG TGACTCTGCT GTCTCTCTCA GCACTCCTGG AAATGTAGAG 
GCTGGCTCTC AGAGTGTGAA CTACAGCTGC AGAAGCAATC TTGCTTCACC AAACCAGTCC 
CAGGACAGCG ATGGTAGGAG TGAATCTAGC TACTCAGGGG AGCGAACACA AGGTGTAGTG 
AGCAGCACAG AGCACTGGCT ATACAAGTCT GCGGAAAATA GTGAGACTGC ATCATGCAAG 
GTAGCTTGTA CCACACCAGG CTGTGCCACT CCTGGTAGCA ACCTGAGCAG CAGCAGCCTG 
GAGAGGACAT CAGCCAAAGA AGATTCCACT TCACTGTATT CTGTAGACCA CGATGGTTAT 
TACAGCTCTA TGAGCATGGA CTCTGGACTG AAGTCAGACA TGCCATGCTA TAGTACTAAT 
GGTTTTGGGA ACCCCAGAGA CAGCGTGATT AATGTCTTTG AAGCAAAGGA GAAGAAACAT 
CAAGATGACC AGCCAGGCCA AGGTGAAAAA TCCCTTGCAA GAAACATCTC TCTAAAAAAG 
GCAAAGAAGC CGCCTTTACC TCCGTCTAGA ACAGACTCAC TCAGAAGGGT ACCCAAGAAG 
AAAGCTCAGT CCAATGGACA GGTACTTGAT GAAACCCTCA TTGCTACCCT CCAGCATTCT 
CTGCAGTTGA ATCTCAAGTG CAAAAATGGC AGCTCCCCTT CCCAGAGCCC CTGCAGTGAC 
TATGAGGATC CCTGGGTGTT GCGCTCTCGC AGCCAAAGCT CGGTCAGCGC AAGCAGCAGC 
ATGATGTCCA CTACTGCCCC AAACCTGTAC TCCATTTGCA CAGTCACTCC CTCTCAGAGT 
GAAACAAGCA GCATCAAGTC AGAGTATGCT GACCAGTGGG GTTACTACAG TGACTATGCT 
GCAGTGGCAG ATGACCAGAT GAAGTCTCCA GTGACCCATT CTGCTAGTAC CTCTTCTGCT 
CTCAGTGATT ACAGCATCAG CCACTTCAGT GATGGCTCAA GGGCTTCTGT ACCACAAGTG 
CCCGCTGGAC TGGCCAAACC AAAGAGCACT TCTCCAGAGA AGTCCCACCG AGTCACATCG 
CCATCCAGTG GGTACTCCAG CCAGTCCAAT ACACCCACTG CACTCACTCC AGTGCCTGTG 
TTTTTAAAAT CTGCGTCATC AGGAAATGGG AAATCCAAGC TGAAACCTAA AGTGCCTGAA 
AGGAAATCTT CTCTTCTGTC TTCAGTCTCC ATTTCTTCAT CCTCTACTTC TCTTTCTTCA 
AATACATCTA CTGAAGGGAG TGTGAATGTG AAGAAATTGG ACCCCACCCC GAGTTCTCCT 
CCAGATTCTG GTGCACCTCT TCCTCCGCCT CCTCTTCTAG CACCTCCTCC ACATTGCCTT 
GAGCTCTCTC CTCCCTCTTC CCCTCCTCCT CCTCCAGCAG AAGCCGTGGA TATATCACCA 
TTGCCAGCTT CTCCCACATT CCCCCCCCCT CCACCAGAAG CTGATGTAAA TTCTTCCTTT 
GCTCACAGTG TCCAGTGGTT ACCACAAGAA GCTTCCATCG GTTCGTACTC TTCCCCTGTT 
CCTCCTCCTG TTTTCCCTTT AGTTGTCCCA CCACCAGCCC CACCTCTTGA TCCCAAACTG 
ACAAAAGGTG CAATGATATG CCCACAGTAT TCATTTAAGA AACATGATCA GGAAGATTCT 
TGCTACAATG CTGTGAAACA GCCATTCAGC AAGCAAGATG CATCAAGACC TGTGATGCCC 
TTGGTAACCA CCAAAGCATT GCAAATGGTG CAGCTGAGGT CTATCAGAAA AGCAACAGAA 
GGTGACCCAT CACCTGAATC TGCTTCTGAA ACCATCTCTC AGGAAAAGGG TATTGTCACT 
TCATCATCAC AGTCTTCCCT AAGGCCATCT CTCTCACTAC GACTCAGTAA CAGCTTAGGT 
GAAGAGGAGA TGAAGATTCA TGGCACTTCA TTTAAAAACA CACCTCAAAC ACTGGCTCAG 
AGTTCTCCTC TGGTACTCTC TGACAACGTA CCCATGCTTG AGAGCGATCA AAAAGCTGCA 
GTTACTATGG GTCTAAGCAA GTCTTTGGAA CCAGATTCCC CAGGGTCAGC TCCAGAAGCC 
ACACCCAAGT CCCCAGTTCG GAGCGAAGAC TTCCATTCTG GCATGCCACT GCAGGGGTCA 
CCAGCATCTC CAGACAAGAC TCAGGTCGTT TTGCCAAACA AGAAACCACC TCCCATTTCA 
AAGAAACCCA AACTGTTCCT TGTTGTACCA CCTCCACAGT TAGATCTCAC AGTGGAGAAA 
ATAGCTGAAG TGAGTGATAC TGTCAGAAGC ACATCAAGCC CAACTAAGAG AGACGCTGTG 
CTTACACACT GCGAGGAGGC GAGAAATTGC CTTACAGATG GACTCAGCTC CAGCGAGATG 
GACTCTGGCA GCCTGGTTCC TGAGGGAGGA GCTGCCAGAG TCACCTTCTC TGAAGCAGTG 
GGAGTTGGTG CCTTTGTGGT ACAGTCCGCT GCATCACCAG TTCAAGAGGA GCCCAGGCAA 
GAGGAGCAGT CAGTTTTTGA TGAAGGAAGC AGCTCAGGCA GCAGCCAAGA CAGCAGCAGT 
AACACTGAAG GACACCCGTC TCAGGAGAAC GAAAGTGCCG AGGTGTTTGA ATCAGAAACA 
GCAAATAGTT CATTCCTGCC AAGCAATAAT TATGGGGAAG AGACGGATGG AGTGGCAACA 
CCAGCGAGAC CGAGGACTAC TGAGGATCTT TTTGCAGCCA TTCACAGATC CAAAAGGAAA 
GTCCTTGGCC GTAAGGATTC TGAGGACGAT CGTACCCGCA ACCATTCTCC TTCACCCCCC 
GTAACACCCA CTGGTGCTTC CCCAACCTTA TCTACCCTCA AACAGGCAGG ATCTATTCAG 
AGAAGCGTTC GCAAGAGCAG CACCAGCAAT GACAACTTCA AAGCCCTGCT GCTGAAGAAA 
GGGAGTCGCT GTGACACCAG TTCCCGCATG TCAGCAGCAG AGATGTTGAA GCACACGGAT 
CCACGGTTCC ACCGAGCCAA GGTGGATGCC TCTCCCGACC TTTCCGACAG CCCTGCCAGC 
TGCTCGCCCA GCAAGAGCAA ACGGGCCCAA GAGGAGTGGG CCAAGAGTGA AGGCCTGATG 
CCGAGGAGCA TGTCCCTCTC CAGCACTAGG TATGGCCGAT CACGAACACC GCCCTCCGCT 
GCCAGCAGCA AGTACAATGT TCGCAACCGC ATCCAGAGCA GCCCCATGAC AGTGATTAGC 
GAAGGAGATG GGGAAGTGGT GGAGCAGACA GAGAGCAGGG TCCAAAGGAC TTTGGATGAG 
CAGCGAGAGA GGCAGCTCGA TGTATTTAAC AGTGATGAAA TAGACATGAA TGACTTTTCG 
TATTCTGAGG AGCTGGACTG CAAGGAGACC TTGGATCCAA CCCATCTGGA CTTAATGACT 
CAGCCGGGTA CTTCGAGAAA GTACCTAAGT CCTTCAGCTG AAGAAAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004935742.1
CDS	1091..6181
Translation	MKKECVSRSFKLKQNAGSLSRAVSWINFSSLSRQTKRLFRSDGELSSICVQQVDEDDENWTYRSPRGKAVSNLDEESRWTVHYTAPWHQQENVFLPSSRPPCVEDLHRQAKLNLKSVLRECDKLRRDGYRSSQYYSQGPTFSSSSSAICGSYQDDYEEIEQKCSVSSPEEEKLITIKRPKTPVSNDLSDINTQTNWTKSLPLPTPEEKMRQQAQAVQTDVVPINVTGENFDRQASIRRSLIYTDTVVRRPKKVKRRKTITGIPDNIQKELAVGTGQSDFRGHSMYVPDHSSTLGRLDSYRSAMQRSETKDTSCQTEEVKVVPPSMRRIRAQKGQGIAAQMSQFSSSSGNMSVMSDSAAVIFASRQNSDIGFHSLPRAGARVSLQSLEQTQSISRQTEDIAGTLPHQMSKLQVDDDVAHLRNNAITGTVSRPKSQEVRSCDSEKSTSPACVVSPHATYSTSVIPNATLSSSSEVIAIHTAQSTGSLDNKISSSAVYPKPRDNPVANNAISGKEDHHSSSGNWSESSSTRHSQTSDTIPSHTVMMLSLGDSAVSLSTPGNVEAGSQSVNYSCRSNLASPNQSQDSDGRSESSYSGERTQGVVSSTEHWLYKSAENSETASCKVACTTPGCATPGSNLSSSSLERTSAKEDSTSLYSVDHDGYYSSMSMDSGLKSDMPCYSTNGFGNPRDSVINVFEAKEKKHQDDQPGQGEKSLARNISLKKAKKPPLPPSRTDSLRRVPKKKAQSNGQVLDETLIATLQHSLQLNLKCKNGSSPSQSPCSDYEDPWVLRSRSQSSVSASSSMMSTTAPNLYSICTVTPSQSETSSIKSEYADQWGYYSDYAAVADDQMKSPVTHSASTSSALSDYSISHFSDGSRASVPQVPAGLAKPKSTSPEKSHRVTSPSSGYSSQSNTPTALTPVPVFLKSASSGNGKSKLKPKVPERKSSLLSSVSISSSSTSLSSNTSTEGSVNVKKLDPTPSSPPDSGAPLPPPPLLAPPPHCLELSPPSSPPPPPAEAVDISPLPASPTFPPPPPEADVNSSFAHSVQWLPQEASIGSYSSPVPPPVFPLVVPPPAPPLDPKLTKGAMICPQYSFKKHDQEDSCYNAVKQPFSKQDASRPVMPLVTTKALQMVQLRSIRKATEGDPSPESASETISQEKGIVTSSSQSSLRPSLSLRLSNSLGEEEMKIHGTSFKNTPQTLAQSSPLVLSDNVPMLESDQKAAVTMGLSKSLEPDSPGSAPEATPKSPVRSEDFHSGMPLQGSPASPDKTQVVLPNKKPPPISKKPKLFLVVPPPQLDLTVEKIAEVSDTVRSTSSPTKRDAVLTHCEEARNCLTDGLSSSEMDSGSLVPEGGAARVTFSEAVGVGAFVVQSAASPVQEEPRQEEQSVFDEGSSSGSSQDSSSNTEGHPSQENESAEVFESETANSSFLPSNNYGEETDGVATPARPRTTEDLFAAIHRSKRKVLGRKDSEDDRTRNHSPSPPVTPTGASPTLSTLKQAGSIQRSVRKSSTSNDNFKALLLKKGSRCDTSSRMSAAEMLKHTDPRFHRAKVDASPDLSDSPASCSPSKSKRAQEEWAKSEGLMPRSMSLSSTRYGRSRTPPSAASSKYNVRNRIQSSPMTVISEGDGEVVEQTESRVQRTLDEQRERQLDVFNSDEIDMNDFSYSEELDCKETLDPTHLDLMTQPGTSRKYLSPSAEES

Target ORF information:

RefSeq Version	XM_004935685.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NHS like 1 (NHSL1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004935685.3

ORF Insert Sequence:

ATGAAAAAGG AGTGTGTTTC GAGATCTTTC AAACTCAAAC AAAACGCAGG CTCCCTCTCG 
CGTGCTGTAA GCTGGATAAA TTTCTCCTCC TTGTCAAGGC AGACAAAGAG ATTGTTTCGC 
AGCGATGGTG AGCTCTCCTC CATTTGCGTG CAGCAGGTAG ACGAGGATGA TGAGAACTGG 
ACCTACAGGA GCCCACGCGG AAAAGCTGTT TCCAACTTAG ATGAAGAGAG CAGATGGACC 
GTCCATTACA CTGCTCCATG GCACCAGCAG GAAAATGTGT TCCTGCCTTC CTCAAGGCCG 
CCTTGTGTGG AGGACTTGCA CCGGCAAGCA AAGCTGAACC TCAAGTCAGT ACTGAGAGAG 
TGTGACAAGC TGCGGAGAGA TGGCTATCGG AGCTCTCAGT ATTACTCTCA GGGCCCCACC 
TTCTCCTCAT CCTCCAGTGC AATCTGTGGA AGTTACCAGG ACGATTATGA AGAAATTGAA 
CAAAAGTGTT CTGTCTCTTC CCCAGAAGAA GAAAAGCTTA TCACCATCAA AAGGCCTAAA 
ACCCCAGTTT CAAATGACTT ATCAGATATC AACACACAAA CCAACTGGAC TAAGTCACTT 
CCACTGCCAA CACCAGAAGA GAAGATGCGA CAGCAAGCAC AGGCAGTCCA AACAGATGTG 
GTTCCTATCA ATGTGACTGG GGAGAATTTC GACCGCCAAG CCAGCATTCG GCGGTCTCTA 
ATTTACACAG ACACGGTGGT AAGACGGCCG AAGAAAGTCA AAAGGAGAAA GACTATAACA 
GGAATCCCTG ACAACATACA AAAGGAGCTA GCAGTTGGCA CAGGCCAAAG TGATTTCCGA 
GGGCACTCGA TGTATGTCCC AGATCACAGT TCCACGCTAG GGCGACTAGA CAGCTATCGC 
TCTGCTATGC AGCGGTCTGA AACCAAGGAC ACCAGCTGCC AGACTGAAGA AGTTAAAGTT 
GTGCCTCCTT CGATGAGAAG AATACGAGCA CAGAAAGGAC AAGGCATTGC AGCTCAGATG 
TCTCAGTTCT CCAGCTCATC TGGAAACATG TCTGTGATGA GTGATTCTGC TGCAGTTATA 
TTTGCTTCTC GCCAGAATAG TGACATAGGT TTTCACAGCT TGCCTCGGGC TGGTGCAAGA 
GTGTCTCTGC AGTCCTTAGA ACAGACCCAG AGCATCTCCA GACAGACAGA AGACATTGCT 
GGTACTTTGC CTCACCAGAT GAGTAAACTG CAAGTGGATG ATGATGTTGC ACATCTGAGA 
AATAATGCCA TAACAGGAAC TGTTTCGAGG CCAAAGTCTC AAGAGGTAAG AAGTTGTGAC 
AGTGAAAAGT CCACTAGCCC AGCATGTGTG GTCTCTCCTC ATGCCACCTA CTCAACAAGC 
GTCATACCCA ATGCGACACT GTCATCCTCC TCAGAAGTTA TTGCTATTCA CACTGCCCAG 
AGTACTGGAT CGCTGGATAA CAAAATTAGC AGTTCTGCTG TGTACCCAAA ACCAAGAGAT 
AATCCTGTTG CCAACAATGC AATAAGTGGG AAAGAAGATC ACCATTCTTC AAGTGGTAAC 
TGGAGTGAGA GTAGCTCCAC ACGTCACTCA CAGACTTCAG ATACCATCCC ATCTCATACT 
GTCATGATGC TTTCTCTTGG TGACTCTGCT GTCTCTCTCA GCACTCCTGG AAATGTAGAG 
GCTGGCTCTC AGAGTGTGAA CTACAGCTGC AGAAGCAATC TTGCTTCACC AAACCAGTCC 
CAGGACAGCG ATGGTAGGAG TGAATCTAGC TACTCAGGGG AGCGAACACA AGGTGTAGTG 
AGCAGCACAG AGCACTGGCT ATACAAGTCT GCGGAAAATA GTGAGACTGC ATCATGCAAG 
GTAGCTTGTA CCACACCAGG CTGTGCCACT CCTGGTAGCA ACCTGAGCAG CAGCAGCCTG 
GAGAGGACAT CAGCCAAAGA AGATTCCACT TCACTGTATT CTGTAGACCA CGATGGTTAT 
TACAGCTCTA TGAGCATGGA CTCTGGACTG AAGTCAGACA TGCCATGCTA TAGTACTAAT 
GGTTTTGGGA ACCCCAGAGA CAGCGTGATT AATGTCTTTG AAGCAAAGGA GAAGAAACAT 
CAAGATGACC AGCCAGGCCA AGGTGAAAAA TCCCTTGCAA GAAACATCTC TCTAAAAAAG 
GCAAAGAAGC CGCCTTTACC TCCGTCTAGA ACAGACTCAC TCAGAAGGGT ACCCAAGAAG 
AAAGCTCAGT CCAATGGACA GGTACTTGAT GAAACCCTCA TTGCTACCCT CCAGCATTCT 
CTGCAGTTGA ATCTCAAGTG CAAAAATGGC AGCTCCCCTT CCCAGAGCCC CTGCAGTGAC 
TATGAGGATC CCTGGGTGTT GCGCTCTCGC AGCCAAAGCT CGGTCAGCGC AAGCAGCAGC 
ATGATGTCCA CTACTGCCCC AAACCTGTAC TCCATTTGCA CAGTCACTCC CTCTCAGAGT 
GAAACAAGCA GCATCAAGTC AGAGTATGCT GACCAGTGGG GTTACTACAG TGACTATGCT 
GCAGTGGCAG ATGACCAGAT GAAGTCTCCA GTGACCCATT CTGCTAGTAC CTCTTCTGCT 
CTCAGTGATT ACAGCATCAG CCACTTCAGT GATGGCTCAA GGGCTTCTGT ACCACAAGTG 
CCCGCTGGAC TGGCCAAACC AAAGAGCACT TCTCCAGAGA AGTCCCACCG AGTCACATCG 
CCATCCAGTG GGTACTCCAG CCAGTCCAAT ACACCCACTG CACTCACTCC AGTGCCTGTG 
TTTTTAAAAT CTGCGTCATC AGGAAATGGG AAATCCAAGC TGAAACCTAA AGTGCCTGAA 
AGGAAATCTT CTCTTCTGTC TTCAGTCTCC ATTTCTTCAT CCTCTACTTC TCTTTCTTCA 
AATACATCTA CTGAAGGGAG TGTGAATGTG AAGAAATTGG ACCCCACCCC GAGTTCTCCT 
CCAGATTCTG GTGCACCTCT TCCTCCGCCT CCTCTTCTAG CACCTCCTCC ACATTGCCTT 
GAGCTCTCTC CTCCCTCTTC CCCTCCTCCT CCTCCAGCAG AAGCCGTGGA TATATCACCA 
TTGCCAGCTT CTCCCACATT CCCCCCCCCT CCACCAGAAG CTGATGTAAA TTCTTCCTTT 
GCTCACAGTG TCCAGTGGTT ACCACAAGAA GCTTCCATCG GTTCGTACTC TTCCCCTGTT 
CCTCCTCCTG TTTTCCCTTT AGTTGTCCCA CCACCAGCCC CACCTCTTGA TCCCAAACTG 
ACAAAAGGTG CAATGATATG CCCACAGTAT TCATTTAAGA AACATGATCA GGAAGATTCT 
TGCTACAATG CTGTGAAACA GCCATTCAGC AAGCAAGATG CATCAAGACC TGTGATGCCC 
TTGGTAACCA CCAAAGCATT GCAAATGGTG CAGCTGAGGT CTATCAGAAA AGCAACAGAA 
GGTGACCCAT CACCTGAATC TGCTTCTGAA ACCATCTCTC AGGAAAAGGG TATTGTCACT 
TCATCATCAC AGTCTTCCCT AAGGCCATCT CTCTCACTAC GACTCAGTAA CAGCTTAGGT 
GAAGAGGAGA TGAAGATTCA TGGCACTTCA TTTAAAAACA CACCTCAAAC ACTGGCTCAG 
AGTTCTCCTC TGGTACTCTC TGACAACGTA CCCATGCTTG AGAGCGATCA AAAAGCTGCA 
GTTACTATGG GTCTAAGCAA GTCTTTGGAA CCAGATTCCC CAGGGTCAGC TCCAGAAGCC 
ACACCCAAGT CCCCAGTTCG GAGCGAAGAC TTCCATTCTG GCATGCCACT GCAGGGGTCA 
CCAGCATCTC CAGACAAGAC TCAGGTCGTT TTGCCAAACA AGAAACCACC TCCCATTTCA 
AAGAAACCCA AACTGTTCCT TGTTGTACCA CCTCCACAGT TAGATCTCAC AGTGGAGAAA 
ATAGCTGAAG TGAGTGATAC TGTCAGAAGC ACATCAAGCC CAACTAAGAG AGACGCTGTG 
CTTACACACT GCGAGGAGGC GAGAAATTGC CTTACAGATG GACTCAGCTC CAGCGAGATG 
GACTCTGGCA GCCTGGTTCC TGAGGGAGGA GCTGCCAGAG TCACCTTCTC TGAAGCAGTG 
GGAGTTGGTG CCTTTGTGGT ACAGTCCGCT GCATCACCAG TTCAAGAGGA GCCCAGGCAA 
GAGGAGCAGT CAGTTTTTGA TGAAGGAAGC AGCTCAGGCA GCAGCCAAGA CAGCAGCAGT 
AACACTGAAG GACACCCGTC TCAGGAGAAC GAAAGTGCCG AGGTGTTTGA ATCAGAAACA 
GCAAATAGTT CATTCCTGCC AAGCAATAAT TATGGGGAAG AGACGGATGG AGTGGCAACA 
CCAGCGAGAC CGAGGACTAC TGAGGATCTT TTTGCAGCCA TTCACAGATC CAAAAGGAAA 
GTCCTTGGCC GTAAGGATTC TGAGGACGAT CGTACCCGCA ACCATTCTCC TTCACCCCCC 
GTAACACCCA CTGGTGCTTC CCCAACCTTA TCTACCCTCA AACAGGCAGG ATCTATTCAG 
AGAAGCGTTC GCAAGAGCAG CACCAGCAAT GACAACTTCA AAGCCCTGCT GCTGAAGAAA 
GGGAGTCGCT GTGACACCAG TTCCCGCATG TCAGCAGCAG AGATGTTGAA GCACACGGAT 
CCACGGTTCC ACCGAGCCAA GGTGGATGCC TCTCCCGACC TTTCCGACAG CCCTGCCAGC 
TGCTCGCCCA GCAAGAGCAA ACGGGCCCAA GAGGAGTGGG CCAAGAGTGA AGGCCTGATG 
CCGAGGAGCA TGTCCCTCTC CAGCACTAGG TATGGCCGAT CACGAACACC GCCCTCCGCT 
GCCAGCAGCA AGTACAATGT TCGCAACCGC ATCCAGAGCA GCCCCATGAC AGTGATTAGC 
GAAGGAGATG GGGAAGTGGT GGAGCAGACA GAGAGCAGGG TCCAAAGGAC TTTGGATGAG 
CAGCGAGAGA GGCAGCTCGA TGTATTTAAC AGTGATGAAA TAGACATGAA TGACTTTTCG 
TATTCTGAGG AGCTGGACTG CAAGGAGACC TTGGATCCAA CCCATCTGGA CTTAATGACT 
CAGCCGGGTA CTTCGAGAAA GTACCTAAGT CCTTCAGCTG AAGAAAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_025149172.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025004940.1 Gallus gallus NHS like 1 (NHSL1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OGa06402
Clone ID Related Accession (Same CDS sequence)	XM_004935685.2 , XM_004935685.3 , XM_025149172.1
Accession Version	XM_025149172.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	NHS-like protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAGG AGTGTGTTTC GAGATCTTTC AAACTCAAAC AAAACGCAGG CTCCCTCTCG 
CGTGCTGTAA GCTGGATAAA TTTCTCCTCC TTGTCAAGGC AGACAAAGAG ATTGTTTCGC 
AGCGATGGTG AGCTCTCCTC CATTTGCGTG CAGCAGGTAG ACGAGGATGA TGAGAACTGG 
ACCTACAGGA GCCCACGCGG AAAAGCTGTT TCCAACTTAG ATGAAGAGAG CAGATGGACC 
GTCCATTACA CTGCTCCATG GCACCAGCAG GAAAATGTGT TCCTGCCTTC CTCAAGGCCG 
CCTTGTGTGG AGGACTTGCA CCGGCAAGCA AAGCTGAACC TCAAGTCAGT ACTGAGAGAG 
TGTGACAAGC TGCGGAGAGA TGGCTATCGG AGCTCTCAGT ATTACTCTCA GGGCCCCACC 
TTCTCCTCAT CCTCCAGTGC AATCTGTGGA AGTTACCAGG ACGATTATGA AGAAATTGAA 
CAAAAGTGTT CTGTCTCTTC CCCAGAAGAA GAAAAGCTTA TCACCATCAA AAGGCCTAAA 
ACCCCAGTTT CAAATGACTT ATCAGATATC AACACACAAA CCAACTGGAC TAAGTCACTT 
CCACTGCCAA CACCAGAAGA GAAGATGCGA CAGCAAGCAC AGGCAGTCCA AACAGATGTG 
GTTCCTATCA ATGTGACTGG GGAGAATTTC GACCGCCAAG CCAGCATTCG GCGGTCTCTA 
ATTTACACAG ACACGGTGGT AAGACGGCCG AAGAAAGTCA AAAGGAGAAA GACTATAACA 
GGAATCCCTG ACAACATACA AAAGGAGCTA GCAGTTGGCA CAGGCCAAAG TGATTTCCGA 
GGGCACTCGA TGTATGTCCC AGATCACAGT TCCACGCTAG GGCGACTAGA CAGCTATCGC 
TCTGCTATGC AGCGGTCTGA AACCAAGGAC ACCAGCTGCC AGACTGAAGA AGTTAAAGTT 
GTGCCTCCTT CGATGAGAAG AATACGAGCA CAGAAAGGAC AAGGCATTGC AGCTCAGATG 
TCTCAGTTCT CCAGCTCATC TGGAAACATG TCTGTGATGA GTGATTCTGC TGCAGTTATA 
TTTGCTTCTC GCCAGAATAG TGACATAGGT TTTCACAGCT TGCCTCGGGC TGGTGCAAGA 
GTGTCTCTGC AGTCCTTAGA ACAGACCCAG AGCATCTCCA GACAGACAGA AGACATTGCT 
GGTACTTTGC CTCACCAGAT GAGTAAACTG CAAGTGGATG ATGATGTTGC ACATCTGAGA 
AATAATGCCA TAACAGGAAC TGTTTCGAGG CCAAAGTCTC AAGAGGTAAG AAGTTGTGAC 
AGTGAAAAGT CCACTAGCCC AGCATGTGTG GTCTCTCCTC ATGCCACCTA CTCAACAAGC 
GTCATACCCA ATGCGACACT GTCATCCTCC TCAGAAGTTA TTGCTATTCA CACTGCCCAG 
AGTACTGGAT CGCTGGATAA CAAAATTAGC AGTTCTGCTG TGTACCCAAA ACCAAGAGAT 
AATCCTGTTG CCAACAATGC AATAAGTGGG AAAGAAGATC ACCATTCTTC AAGTGGTAAC 
TGGAGTGAGA GTAGCTCCAC ACGTCACTCA CAGACTTCAG ATACCATCCC ATCTCATACT 
GTCATGATGC TTTCTCTTGG TGACTCTGCT GTCTCTCTCA GCACTCCTGG AAATGTAGAG 
GCTGGCTCTC AGAGTGTGAA CTACAGCTGC AGAAGCAATC TTGCTTCACC AAACCAGTCC 
CAGGACAGCG ATGGTAGGAG TGAATCTAGC TACTCAGGGG AGCGAACACA AGGTGTAGTG 
AGCAGCACAG AGCACTGGCT ATACAAGTCT GCGGAAAATA GTGAGACTGC ATCATGCAAG 
GTAGCTTGTA CCACACCAGG CTGTGCCACT CCTGGTAGCA ACCTGAGCAG CAGCAGCCTG 
GAGAGGACAT CAGCCAAAGA AGATTCCACT TCACTGTATT CTGTAGACCA CGATGGTTAT 
TACAGCTCTA TGAGCATGGA CTCTGGACTG AAGTCAGACA TGCCATGCTA TAGTACTAAT 
GGTTTTGGGA ACCCCAGAGA CAGCGTGATT AATGTCTTTG AAGCAAAGGA GAAGAAACAT 
CAAGATGACC AGCCAGGCCA AGGTGAAAAA TCCCTTGCAA GAAACATCTC TCTAAAAAAG 
GCAAAGAAGC CGCCTTTACC TCCGTCTAGA ACAGACTCAC TCAGAAGGGT ACCCAAGAAG 
AAAGCTCAGT CCAATGGACA GGTACTTGAT GAAACCCTCA TTGCTACCCT CCAGCATTCT 
CTGCAGTTGA ATCTCAAGTG CAAAAATGGC AGCTCCCCTT CCCAGAGCCC CTGCAGTGAC 
TATGAGGATC CCTGGGTGTT GCGCTCTCGC AGCCAAAGCT CGGTCAGCGC AAGCAGCAGC 
ATGATGTCCA CTACTGCCCC AAACCTGTAC TCCATTTGCA CAGTCACTCC CTCTCAGAGT 
GAAACAAGCA GCATCAAGTC AGAGTATGCT GACCAGTGGG GTTACTACAG TGACTATGCT 
GCAGTGGCAG ATGACCAGAT GAAGTCTCCA GTGACCCATT CTGCTAGTAC CTCTTCTGCT 
CTCAGTGATT ACAGCATCAG CCACTTCAGT GATGGCTCAA GGGCTTCTGT ACCACAAGTG 
CCCGCTGGAC TGGCCAAACC AAAGAGCACT TCTCCAGAGA AGTCCCACCG AGTCACATCG 
CCATCCAGTG GGTACTCCAG CCAGTCCAAT ACACCCACTG CACTCACTCC AGTGCCTGTG 
TTTTTAAAAT CTGCGTCATC AGGAAATGGG AAATCCAAGC TGAAACCTAA AGTGCCTGAA 
AGGAAATCTT CTCTTCTGTC TTCAGTCTCC ATTTCTTCAT CCTCTACTTC TCTTTCTTCA 
AATACATCTA CTGAAGGGAG TGTGAATGTG AAGAAATTGG ACCCCACCCC GAGTTCTCCT 
CCAGATTCTG GTGCACCTCT TCCTCCGCCT CCTCTTCTAG CACCTCCTCC ACATTGCCTT 
GAGCTCTCTC CTCCCTCTTC CCCTCCTCCT CCTCCAGCAG AAGCCGTGGA TATATCACCA 
TTGCCAGCTT CTCCCACATT CCCCCCCCCT CCACCAGAAG CTGATGTAAA TTCTTCCTTT 
GCTCACAGTG TCCAGTGGTT ACCACAAGAA GCTTCCATCG GTTCGTACTC TTCCCCTGTT 
CCTCCTCCTG TTTTCCCTTT AGTTGTCCCA CCACCAGCCC CACCTCTTGA TCCCAAACTG 
ACAAAAGGTG CAATGATATG CCCACAGTAT TCATTTAAGA AACATGATCA GGAAGATTCT 
TGCTACAATG CTGTGAAACA GCCATTCAGC AAGCAAGATG CATCAAGACC TGTGATGCCC 
TTGGTAACCA CCAAAGCATT GCAAATGGTG CAGCTGAGGT CTATCAGAAA AGCAACAGAA 
GGTGACCCAT CACCTGAATC TGCTTCTGAA ACCATCTCTC AGGAAAAGGG TATTGTCACT 
TCATCATCAC AGTCTTCCCT AAGGCCATCT CTCTCACTAC GACTCAGTAA CAGCTTAGGT 
GAAGAGGAGA TGAAGATTCA TGGCACTTCA TTTAAAAACA CACCTCAAAC ACTGGCTCAG 
AGTTCTCCTC TGGTACTCTC TGACAACGTA CCCATGCTTG AGAGCGATCA AAAAGCTGCA 
GTTACTATGG GTCTAAGCAA GTCTTTGGAA CCAGATTCCC CAGGGTCAGC TCCAGAAGCC 
ACACCCAAGT CCCCAGTTCG GAGCGAAGAC TTCCATTCTG GCATGCCACT GCAGGGGTCA 
CCAGCATCTC CAGACAAGAC TCAGGTCGTT TTGCCAAACA AGAAACCACC TCCCATTTCA 
AAGAAACCCA AACTGTTCCT TGTTGTACCA CCTCCACAGT TAGATCTCAC AGTGGAGAAA 
ATAGCTGAAG TGAGTGATAC TGTCAGAAGC ACATCAAGCC CAACTAAGAG AGACGCTGTG 
CTTACACACT GCGAGGAGGC GAGAAATTGC CTTACAGATG GACTCAGCTC CAGCGAGATG 
GACTCTGGCA GCCTGGTTCC TGAGGGAGGA GCTGCCAGAG TCACCTTCTC TGAAGCAGTG 
GGAGTTGGTG CCTTTGTGGT ACAGTCCGCT GCATCACCAG TTCAAGAGGA GCCCAGGCAA 
GAGGAGCAGT CAGTTTTTGA TGAAGGAAGC AGCTCAGGCA GCAGCCAAGA CAGCAGCAGT 
AACACTGAAG GACACCCGTC TCAGGAGAAC GAAAGTGCCG AGGTGTTTGA ATCAGAAACA 
GCAAATAGTT CATTCCTGCC AAGCAATAAT TATGGGGAAG AGACGGATGG AGTGGCAACA 
CCAGCGAGAC CGAGGACTAC TGAGGATCTT TTTGCAGCCA TTCACAGATC CAAAAGGAAA 
GTCCTTGGCC GTAAGGATTC TGAGGACGAT CGTACCCGCA ACCATTCTCC TTCACCCCCC 
GTAACACCCA CTGGTGCTTC CCCAACCTTA TCTACCCTCA AACAGGCAGG ATCTATTCAG 
AGAAGCGTTC GCAAGAGCAG CACCAGCAAT GACAACTTCA AAGCCCTGCT GCTGAAGAAA 
GGGAGTCGCT GTGACACCAG TTCCCGCATG TCAGCAGCAG AGATGTTGAA GCACACGGAT 
CCACGGTTCC ACCGAGCCAA GGTGGATGCC TCTCCCGACC TTTCCGACAG CCCTGCCAGC 
TGCTCGCCCA GCAAGAGCAA ACGGGCCCAA GAGGAGTGGG CCAAGAGTGA AGGCCTGATG 
CCGAGGAGCA TGTCCCTCTC CAGCACTAGG TATGGCCGAT CACGAACACC GCCCTCCGCT 
GCCAGCAGCA AGTACAATGT TCGCAACCGC ATCCAGAGCA GCCCCATGAC AGTGATTAGC 
GAAGGAGATG GGGAAGTGGT GGAGCAGACA GAGAGCAGGG TCCAAAGGAC TTTGGATGAG 
CAGCGAGAGA GGCAGCTCGA TGTATTTAAC AGTGATGAAA TAGACATGAA TGACTTTTCG 
TATTCTGAGG AGCTGGACTG CAAGGAGACC TTGGATCCAA CCCATCTGGA CTTAATGACT 
CAGCCGGGTA CTTCGAGAAA GTACCTAAGT CCTTCAGCTG AAGAAAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025004940.1
CDS	1338..6428
Translation	MKKECVSRSFKLKQNAGSLSRAVSWINFSSLSRQTKRLFRSDGELSSICVQQVDEDDENWTYRSPRGKAVSNLDEESRWTVHYTAPWHQQENVFLPSSRPPCVEDLHRQAKLNLKSVLRECDKLRRDGYRSSQYYSQGPTFSSSSSAICGSYQDDYEEIEQKCSVSSPEEEKLITIKRPKTPVSNDLSDINTQTNWTKSLPLPTPEEKMRQQAQAVQTDVVPINVTGENFDRQASIRRSLIYTDTVVRRPKKVKRRKTITGIPDNIQKELAVGTGQSDFRGHSMYVPDHSSTLGRLDSYRSAMQRSETKDTSCQTEEVKVVPPSMRRIRAQKGQGIAAQMSQFSSSSGNMSVMSDSAAVIFASRQNSDIGFHSLPRAGARVSLQSLEQTQSISRQTEDIAGTLPHQMSKLQVDDDVAHLRNNAITGTVSRPKSQEVRSCDSEKSTSPACVVSPHATYSTSVIPNATLSSSSEVIAIHTAQSTGSLDNKISSSAVYPKPRDNPVANNAISGKEDHHSSSGNWSESSSTRHSQTSDTIPSHTVMMLSLGDSAVSLSTPGNVEAGSQSVNYSCRSNLASPNQSQDSDGRSESSYSGERTQGVVSSTEHWLYKSAENSETASCKVACTTPGCATPGSNLSSSSLERTSAKEDSTSLYSVDHDGYYSSMSMDSGLKSDMPCYSTNGFGNPRDSVINVFEAKEKKHQDDQPGQGEKSLARNISLKKAKKPPLPPSRTDSLRRVPKKKAQSNGQVLDETLIATLQHSLQLNLKCKNGSSPSQSPCSDYEDPWVLRSRSQSSVSASSSMMSTTAPNLYSICTVTPSQSETSSIKSEYADQWGYYSDYAAVADDQMKSPVTHSASTSSALSDYSISHFSDGSRASVPQVPAGLAKPKSTSPEKSHRVTSPSSGYSSQSNTPTALTPVPVFLKSASSGNGKSKLKPKVPERKSSLLSSVSISSSSTSLSSNTSTEGSVNVKKLDPTPSSPPDSGAPLPPPPLLAPPPHCLELSPPSSPPPPPAEAVDISPLPASPTFPPPPPEADVNSSFAHSVQWLPQEASIGSYSSPVPPPVFPLVVPPPAPPLDPKLTKGAMICPQYSFKKHDQEDSCYNAVKQPFSKQDASRPVMPLVTTKALQMVQLRSIRKATEGDPSPESASETISQEKGIVTSSSQSSLRPSLSLRLSNSLGEEEMKIHGTSFKNTPQTLAQSSPLVLSDNVPMLESDQKAAVTMGLSKSLEPDSPGSAPEATPKSPVRSEDFHSGMPLQGSPASPDKTQVVLPNKKPPPISKKPKLFLVVPPPQLDLTVEKIAEVSDTVRSTSSPTKRDAVLTHCEEARNCLTDGLSSSEMDSGSLVPEGGAARVTFSEAVGVGAFVVQSAASPVQEEPRQEEQSVFDEGSSSGSSQDSSSNTEGHPSQENESAEVFESETANSSFLPSNNYGEETDGVATPARPRTTEDLFAAIHRSKRKVLGRKDSEDDRTRNHSPSPPVTPTGASPTLSTLKQAGSIQRSVRKSSTSNDNFKALLLKKGSRCDTSSRMSAAEMLKHTDPRFHRAKVDASPDLSDSPASCSPSKSKRAQEEWAKSEGLMPRSMSLSSTRYGRSRTPPSAASSKYNVRNRIQSSPMTVISEGDGEVVEQTESRVQRTLDEQRERQLDVFNSDEIDMNDFSYSEELDCKETLDPTHLDLMTQPGTSRKYLSPSAEES

Target ORF information:

RefSeq Version	XM_025149172.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NHS like 1 (NHSL1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025149172.1

ORF Insert Sequence:

ATGAAAAAGG AGTGTGTTTC GAGATCTTTC AAACTCAAAC AAAACGCAGG CTCCCTCTCG 
CGTGCTGTAA GCTGGATAAA TTTCTCCTCC TTGTCAAGGC AGACAAAGAG ATTGTTTCGC 
AGCGATGGTG AGCTCTCCTC CATTTGCGTG CAGCAGGTAG ACGAGGATGA TGAGAACTGG 
ACCTACAGGA GCCCACGCGG AAAAGCTGTT TCCAACTTAG ATGAAGAGAG CAGATGGACC 
GTCCATTACA CTGCTCCATG GCACCAGCAG GAAAATGTGT TCCTGCCTTC CTCAAGGCCG 
CCTTGTGTGG AGGACTTGCA CCGGCAAGCA AAGCTGAACC TCAAGTCAGT ACTGAGAGAG 
TGTGACAAGC TGCGGAGAGA TGGCTATCGG AGCTCTCAGT ATTACTCTCA GGGCCCCACC 
TTCTCCTCAT CCTCCAGTGC AATCTGTGGA AGTTACCAGG ACGATTATGA AGAAATTGAA 
CAAAAGTGTT CTGTCTCTTC CCCAGAAGAA GAAAAGCTTA TCACCATCAA AAGGCCTAAA 
ACCCCAGTTT CAAATGACTT ATCAGATATC AACACACAAA CCAACTGGAC TAAGTCACTT 
CCACTGCCAA CACCAGAAGA GAAGATGCGA CAGCAAGCAC AGGCAGTCCA AACAGATGTG 
GTTCCTATCA ATGTGACTGG GGAGAATTTC GACCGCCAAG CCAGCATTCG GCGGTCTCTA 
ATTTACACAG ACACGGTGGT AAGACGGCCG AAGAAAGTCA AAAGGAGAAA GACTATAACA 
GGAATCCCTG ACAACATACA AAAGGAGCTA GCAGTTGGCA CAGGCCAAAG TGATTTCCGA 
GGGCACTCGA TGTATGTCCC AGATCACAGT TCCACGCTAG GGCGACTAGA CAGCTATCGC 
TCTGCTATGC AGCGGTCTGA AACCAAGGAC ACCAGCTGCC AGACTGAAGA AGTTAAAGTT 
GTGCCTCCTT CGATGAGAAG AATACGAGCA CAGAAAGGAC AAGGCATTGC AGCTCAGATG 
TCTCAGTTCT CCAGCTCATC TGGAAACATG TCTGTGATGA GTGATTCTGC TGCAGTTATA 
TTTGCTTCTC GCCAGAATAG TGACATAGGT TTTCACAGCT TGCCTCGGGC TGGTGCAAGA 
GTGTCTCTGC AGTCCTTAGA ACAGACCCAG AGCATCTCCA GACAGACAGA AGACATTGCT 
GGTACTTTGC CTCACCAGAT GAGTAAACTG CAAGTGGATG ATGATGTTGC ACATCTGAGA 
AATAATGCCA TAACAGGAAC TGTTTCGAGG CCAAAGTCTC AAGAGGTAAG AAGTTGTGAC 
AGTGAAAAGT CCACTAGCCC AGCATGTGTG GTCTCTCCTC ATGCCACCTA CTCAACAAGC 
GTCATACCCA ATGCGACACT GTCATCCTCC TCAGAAGTTA TTGCTATTCA CACTGCCCAG 
AGTACTGGAT CGCTGGATAA CAAAATTAGC AGTTCTGCTG TGTACCCAAA ACCAAGAGAT 
AATCCTGTTG CCAACAATGC AATAAGTGGG AAAGAAGATC ACCATTCTTC AAGTGGTAAC 
TGGAGTGAGA GTAGCTCCAC ACGTCACTCA CAGACTTCAG ATACCATCCC ATCTCATACT 
GTCATGATGC TTTCTCTTGG TGACTCTGCT GTCTCTCTCA GCACTCCTGG AAATGTAGAG 
GCTGGCTCTC AGAGTGTGAA CTACAGCTGC AGAAGCAATC TTGCTTCACC AAACCAGTCC 
CAGGACAGCG ATGGTAGGAG TGAATCTAGC TACTCAGGGG AGCGAACACA AGGTGTAGTG 
AGCAGCACAG AGCACTGGCT ATACAAGTCT GCGGAAAATA GTGAGACTGC ATCATGCAAG 
GTAGCTTGTA CCACACCAGG CTGTGCCACT CCTGGTAGCA ACCTGAGCAG CAGCAGCCTG 
GAGAGGACAT CAGCCAAAGA AGATTCCACT TCACTGTATT CTGTAGACCA CGATGGTTAT 
TACAGCTCTA TGAGCATGGA CTCTGGACTG AAGTCAGACA TGCCATGCTA TAGTACTAAT 
GGTTTTGGGA ACCCCAGAGA CAGCGTGATT AATGTCTTTG AAGCAAAGGA GAAGAAACAT 
CAAGATGACC AGCCAGGCCA AGGTGAAAAA TCCCTTGCAA GAAACATCTC TCTAAAAAAG 
GCAAAGAAGC CGCCTTTACC TCCGTCTAGA ACAGACTCAC TCAGAAGGGT ACCCAAGAAG 
AAAGCTCAGT CCAATGGACA GGTACTTGAT GAAACCCTCA TTGCTACCCT CCAGCATTCT 
CTGCAGTTGA ATCTCAAGTG CAAAAATGGC AGCTCCCCTT CCCAGAGCCC CTGCAGTGAC 
TATGAGGATC CCTGGGTGTT GCGCTCTCGC AGCCAAAGCT CGGTCAGCGC AAGCAGCAGC 
ATGATGTCCA CTACTGCCCC AAACCTGTAC TCCATTTGCA CAGTCACTCC CTCTCAGAGT 
GAAACAAGCA GCATCAAGTC AGAGTATGCT GACCAGTGGG GTTACTACAG TGACTATGCT 
GCAGTGGCAG ATGACCAGAT GAAGTCTCCA GTGACCCATT CTGCTAGTAC CTCTTCTGCT 
CTCAGTGATT ACAGCATCAG CCACTTCAGT GATGGCTCAA GGGCTTCTGT ACCACAAGTG 
CCCGCTGGAC TGGCCAAACC AAAGAGCACT TCTCCAGAGA AGTCCCACCG AGTCACATCG 
CCATCCAGTG GGTACTCCAG CCAGTCCAAT ACACCCACTG CACTCACTCC AGTGCCTGTG 
TTTTTAAAAT CTGCGTCATC AGGAAATGGG AAATCCAAGC TGAAACCTAA AGTGCCTGAA 
AGGAAATCTT CTCTTCTGTC TTCAGTCTCC ATTTCTTCAT CCTCTACTTC TCTTTCTTCA 
AATACATCTA CTGAAGGGAG TGTGAATGTG AAGAAATTGG ACCCCACCCC GAGTTCTCCT 
CCAGATTCTG GTGCACCTCT TCCTCCGCCT CCTCTTCTAG CACCTCCTCC ACATTGCCTT 
GAGCTCTCTC CTCCCTCTTC CCCTCCTCCT CCTCCAGCAG AAGCCGTGGA TATATCACCA 
TTGCCAGCTT CTCCCACATT CCCCCCCCCT CCACCAGAAG CTGATGTAAA TTCTTCCTTT 
GCTCACAGTG TCCAGTGGTT ACCACAAGAA GCTTCCATCG GTTCGTACTC TTCCCCTGTT 
CCTCCTCCTG TTTTCCCTTT AGTTGTCCCA CCACCAGCCC CACCTCTTGA TCCCAAACTG 
ACAAAAGGTG CAATGATATG CCCACAGTAT TCATTTAAGA AACATGATCA GGAAGATTCT 
TGCTACAATG CTGTGAAACA GCCATTCAGC AAGCAAGATG CATCAAGACC TGTGATGCCC 
TTGGTAACCA CCAAAGCATT GCAAATGGTG CAGCTGAGGT CTATCAGAAA AGCAACAGAA 
GGTGACCCAT CACCTGAATC TGCTTCTGAA ACCATCTCTC AGGAAAAGGG TATTGTCACT 
TCATCATCAC AGTCTTCCCT AAGGCCATCT CTCTCACTAC GACTCAGTAA CAGCTTAGGT 
GAAGAGGAGA TGAAGATTCA TGGCACTTCA TTTAAAAACA CACCTCAAAC ACTGGCTCAG 
AGTTCTCCTC TGGTACTCTC TGACAACGTA CCCATGCTTG AGAGCGATCA AAAAGCTGCA 
GTTACTATGG GTCTAAGCAA GTCTTTGGAA CCAGATTCCC CAGGGTCAGC TCCAGAAGCC 
ACACCCAAGT CCCCAGTTCG GAGCGAAGAC TTCCATTCTG GCATGCCACT GCAGGGGTCA 
CCAGCATCTC CAGACAAGAC TCAGGTCGTT TTGCCAAACA AGAAACCACC TCCCATTTCA 
AAGAAACCCA AACTGTTCCT TGTTGTACCA CCTCCACAGT TAGATCTCAC AGTGGAGAAA 
ATAGCTGAAG TGAGTGATAC TGTCAGAAGC ACATCAAGCC CAACTAAGAG AGACGCTGTG 
CTTACACACT GCGAGGAGGC GAGAAATTGC CTTACAGATG GACTCAGCTC CAGCGAGATG 
GACTCTGGCA GCCTGGTTCC TGAGGGAGGA GCTGCCAGAG TCACCTTCTC TGAAGCAGTG 
GGAGTTGGTG CCTTTGTGGT ACAGTCCGCT GCATCACCAG TTCAAGAGGA GCCCAGGCAA 
GAGGAGCAGT CAGTTTTTGA TGAAGGAAGC AGCTCAGGCA GCAGCCAAGA CAGCAGCAGT 
AACACTGAAG GACACCCGTC TCAGGAGAAC GAAAGTGCCG AGGTGTTTGA ATCAGAAACA 
GCAAATAGTT CATTCCTGCC AAGCAATAAT TATGGGGAAG AGACGGATGG AGTGGCAACA 
CCAGCGAGAC CGAGGACTAC TGAGGATCTT TTTGCAGCCA TTCACAGATC CAAAAGGAAA 
GTCCTTGGCC GTAAGGATTC TGAGGACGAT CGTACCCGCA ACCATTCTCC TTCACCCCCC 
GTAACACCCA CTGGTGCTTC CCCAACCTTA TCTACCCTCA AACAGGCAGG ATCTATTCAG 
AGAAGCGTTC GCAAGAGCAG CACCAGCAAT GACAACTTCA AAGCCCTGCT GCTGAAGAAA 
GGGAGTCGCT GTGACACCAG TTCCCGCATG TCAGCAGCAG AGATGTTGAA GCACACGGAT 
CCACGGTTCC ACCGAGCCAA GGTGGATGCC TCTCCCGACC TTTCCGACAG CCCTGCCAGC 
TGCTCGCCCA GCAAGAGCAA ACGGGCCCAA GAGGAGTGGG CCAAGAGTGA AGGCCTGATG 
CCGAGGAGCA TGTCCCTCTC CAGCACTAGG TATGGCCGAT CACGAACACC GCCCTCCGCT 
GCCAGCAGCA AGTACAATGT TCGCAACCGC ATCCAGAGCA GCCCCATGAC AGTGATTAGC 
GAAGGAGATG GGGAAGTGGT GGAGCAGACA GAGAGCAGGG TCCAAAGGAC TTTGGATGAG 
CAGCGAGAGA GGCAGCTCGA TGTATTTAAC AGTGATGAAA TAGACATGAA TGACTTTTCG 
TATTCTGAGG AGCTGGACTG CAAGGAGACC TTGGATCCAA CCCATCTGGA CTTAATGACT 
CAGCCGGGTA CTTCGAGAAA GTACCTAAGT CCTTCAGCTG AAGAAAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_004935687.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004935744.1 Gallus gallus NHS-like 1 (NHSL1), transcript variant X6, mRNA.

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CloneID	OGa06404
Clone ID Related Accession (Same CDS sequence)	XM_004935687.2 , XM_004935687.3
Accession Version	XM_004935687.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	NHS-like protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455837.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004935687.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCGCCT ATGTGCGCTG CCTGCCTGCA GAGCCGATGG TCGGGTGCCT CAGCAGCAGC 
AAGTGGCAGC CGGGCTGCAA GGATGAGGAT GAGGAGGAAC TCATCCCACT GCATGCTGGA 
AGGATGGGCA ACGCGTACCC CAAGTTGCCC GGGCGCCTCA AGAGGAAGCT GCTGAGAAAG 
GAGGATGACA GCTTCTGGCC GACTGTTTCC AACTTAGATG AAGAGAGCAG ATGGACCGTC 
CATTACACTG CTCCATGGCA CCAGCAGGAA AATGTGTTCC TGCCTTCCTC AAGGCCGCCT 
TGTGTGGAGG ACTTGCACCG GCAAGCAAAG CTGAACCTCA AGTCAGTACT GAGAGAGTGT 
GACAAGCTGC GGAGAGATGG CTATCGGAGC TCTCAGTATT ACTCTCAGGG CCCCACCTTC 
TCCTCATCCT CCAGTGCAAT CTGTGGAAGT TACCAGGACG ATTATGAAGA AATTGAACAA 
AAGTGTTCTG TCTCTTCCCC AGAAGAAGAA AAGCTTATCA CCATCAAAAG GCCTAAAACC 
CCAGTTTCAA ATGACTTATC AGATATCAAC ACACAAACCA ACTGGACTAA GTCACTTCCA 
CTGCCAACAC CAGAAGAGAA GATGCGACAG CAAGCACAGG CAGTCCAAAC AGATGTGGTT 
CCTATCAATG TGACTGGGGA GAATTTCGAC CGCCAAGCCA GCATTCGGCG GTCTCTAATT 
TACACAGACA CGGTGGTAAG ACGGCCGAAG AAAGTCAAAA GGAGAAAGAC TATAACAGGA 
ATCCCTGACA ACATACAAAA GGAGCTAGCA GTTGGCACAG GCCAAAGTGA TTTCCGAGGG 
CACTCGATGT ATGTCCCAGA TCACAGTTCC ACGCTAGGGC GACTAGACAG CTATCGCTCT 
GCTATGCAGC GGTCTGAAAC CAAGGACACC AGCTGCCAGA CTGAAGAAGT TAAAGTTGTG 
CCTCCTTCGA TGAGAAGAAT ACGAGCACAG AAAGGACAAG GCATTGCAGC TCAGATGTCT 
CAGTTCTCCA GCTCATCTGG AAACATGTCT GTGATGAGTG ATTCTGCTGC AGTTATATTT 
GCTTCTCGCC AGAATAGTGA CATAGGTTTT CACAGCTTGC CTCGGGCTGG TGCAAGAGTG 
TCTCTGCAGT CCTTAGAACA GACCCAGAGC ATCTCCAGAC AGACAGAAGA CATTGCTGGT 
ACTTTGCCTC ACCAGATGAG TAAACTGCAA GTGGATGATG ATGTTGCACA TCTGAGAAAT 
AATGCCATAA CAGGAACTGT TTCGAGGCCA AAGTCTCAAG AGGTAAGAAG TTGTGACAGT 
GAAAAGTCCA CTAGCCCAGC ATGTGTGGTC TCTCCTCATG CCACCTACTC AACAAGCGTC 
ATACCCAATG CGACACTGTC ATCCTCCTCA GAAGTTATTG CTATTCACAC TGCCCAGAGT 
ACTGGATCGC TGGATAACAA AATTAGCAGT TCTGCTGTGT ACCCAAAACC AAGAGATAAT 
CCTGTTGCCA ACAATGCAAT AAGTGGGAAA GAAGATCACC ATTCTTCAAG TGGTAACTGG 
AGTGAGAGTA GCTCCACACG TCACTCACAG ACTTCAGATA CCATCCCATC TCATACTGTC 
ATGATGCTTT CTCTTGGTGA CTCTGCTGTC TCTCTCAGCA CTCCTGGAAA TGTAGAGGCT 
GGCTCTCAGA GTGTGAACTA CAGCTGCAGA AGCAATCTTG CTTCACCAAA CCAGTCCCAG 
GACAGCGATG GTAGGAGTGA ATCTAGCTAC TCAGGGGAGC GAACACAAGG TGTAGTGAGC 
AGCACAGAGC ACTGGCTATA CAAGTCTGCG GAAAATAGTG AGACTGCATC ATGCAAGGTA 
GCTTGTACCA CACCAGGCTG TGCCACTCCT GGTAGCAACC TGAGCAGCAG CAGCCTGGAG 
AGGACATCAG CCAAAGAAGA TTCCACTTCA CTGTATTCTG TAGACCACGA TGGTTATTAC 
AGCTCTATGA GCATGGACTC TGGACTGAAG TCAGACATGC CATGCTATAG TACTAATGGT 
TTTGGGAACC CCAGAGACAG CGTGATTAAT GTCTTTGAAG CAAAGGAGAA GAAACATCAA 
GATGACCAGC CAGGCCAAGG TGAAAAATCC CTTGCAAGAA ACATCTCTCT AAAAAAGGCA 
AAGAAGCCGC CTTTACCTCC GTCTAGAACA GACTCACTCA GAAGGGTACC CAAGAAGAAA 
GCTCAGTCCA ATGGACAGGT ACTTGATGAA ACCCTCATTG CTACCCTCCA GCATTCTCTG 
CAGTTGAATC TCAAGTGCAA AAATGGCAGC TCCCCTTCCC AGAGCCCCTG CAGTGACTAT 
GAGGATCCCT GGGTGTTGCG CTCTCGCAGC CAAAGCTCGG TCAGCGCAAG CAGCAGCATG 
ATGTCCACTA CTGCCCCAAA CCTGTACTCC ATTTGCACAG TCACTCCCTC TCAGAGTGAA 
ACAAGCAGCA TCAAGTCAGA GTATGCTGAC CAGTGGGGTT ACTACAGTGA CTATGCTGCA 
GTGGCAGATG ACCAGATGAA GTCTCCAGTG ACCCATTCTG CTAGTACCTC TTCTGCTCTC 
AGTGATTACA GCATCAGCCA CTTCAGTGAT GGCTCAAGGG CTTCTGTACC ACAAGTGCCC 
GCTGGACTGG CCAAACCAAA GAGCACTTCT CCAGAGAAGT CCCACCGAGT CACATCGCCA 
TCCAGTGGGT ACTCCAGCCA GTCCAATACA CCCACTGCAC TCACTCCAGT GCCTGTGTTT 
TTAAAATCTG CGTCATCAGG AAATGGGAAA TCCAAGCTGA AACCTAAAGT GCCTGAAAGG 
AAATCTTCTC TTCTGTCTTC AGTCTCCATT TCTTCATCCT CTACTTCTCT TTCTTCAAAT 
ACATCTACTG AAGGGAGTGT GAATGTGAAG AAATTGGACC CCACCCCGAG TTCTCCTCCA 
GATTCTGGTG CACCTCTTCC TCCGCCTCCT CTTCTAGCAC CTCCTCCACA TTGCCTTGAG 
CTCTCTCCTC CCTCTTCCCC TCCTCCTCCT CCAGCAGAAG CCGTGGATAT ATCACCATTG 
CCAGCTTCTC CCACATTCCC CCCCCCTCCA CCAGAAGCTG ATGTAAATTC TTCCTTTGCT 
CACAGTGTCC AGTGGTTACC ACAAGAAGCT TCCATCGGTT CGTACTCTTC CCCTGTTCCT 
CCTCCTGTTT TCCCTTTAGT TGTCCCACCA CCAGCCCCAC CTCTTGATCC CAAACTGACA 
AAAGGTGCAA TGATATGCCC ACAGTATTCA TTTAAGAAAC ATGATCAGGA AGATTCTTGC 
TACAATGCTG TGAAACAGCC ATTCAGCAAG CAAGATGCAT CAAGACCTGT GATGCCCTTG 
GTAACCACCA AAGCATTGCA AATGGTGCAG CTGAGGTCTA TCAGAAAAGC AACAGAAGGT 
GACCCATCAC CTGAATCTGC TTCTGAAACC ATCTCTCAGG AAAAGGGTAT TGTCACTTCA 
TCATCACAGT CTTCCCTAAG GCCATCTCTC TCACTACGAC TCAGTAACAG CTTAGGTGAA 
GAGGAGATGA AGATTCATGG CACTTCATTT AAAAACACAC CTCAAACACT GGCTCAGAGT 
TCTCCTCTGG TACTCTCTGA CAACGTACCC ATGCTTGAGA GCGATCAAAA AGCTGCAGTT 
ACTATGGGTC TAAGCAAGTC TTTGGAACCA GATTCCCCAG GGTCAGCTCC AGAAGCCACA 
CCCAAGTCCC CAGTTCGGAG CGAAGACTTC CATTCTGGCA TGCCACTGCA GGGGTCACCA 
GCATCTCCAG ACAAGACTCA GGTCGTTTTG CCAAACAAGA AACCACCTCC CATTTCAAAG 
AAACCCAAAC TGTTCCTTGT TGTACCACCT CCACAGTTAG ATCTCACAGT GGAGAAAATA 
GCTGAAGTGA GTGATACTGT CAGAAGCACA TCAAGCCCAA CTAAGAGAGA CGCTGTGCTT 
ACACACTGCG AGGAGGCGAG AAATTGCCTT ACAGATGGAC TCAGCTCCAG CGAGATGGAC 
TCTGGCAGCC TGGTTCCTGA GGGAGGAGCT GCCAGAGTCA CCTTCTCTGA AGCAGTGGGA 
GTTGGTGCCT TTGTGGTACA GTCCGCTGCA TCACCAGTTC AAGAGGAGCC CAGGCAAGAG 
GAGCAGTCAG TTTTTGATGA AGGAAGCAGC TCAGGCAGCA GCCAAGACAG CAGCAGTAAC 
ACTGAAGGAC ACCCGTCTCA GGAGAACGAA AGTGCCGAGG TGTTTGAATC AGAAACAGCA 
AATAGTTCAT TCCTGCCAAG CAATAATTAT GGGGAAGAGA CGGATGGAGT GGCAACACCA 
GCGAGACCGA GGACTACTGA GGATCTTTTT GCAGCCATTC ACAGATCCAA AAGGAAAGTC 
CTTGGCCGTA AGGATTCTGA GGACGATCGT ACCCGCAACC ATTCTCCTTC ACCCCCCGTA 
ACACCCACTG GTGCTTCCCC AACCTTATCT ACCCTCAAAC AGGCAGGATC TATTCAGAGA 
AGCGTTCGCA AGAGCAGCAC CAGCAATGAC AACTTCAAAG CCCTGCTGCT GAAGAAAGGG 
AGTCGCTGTG ACACCAGTTC CCGCATGTCA GCAGCAGAGA TGTTGAAGCA CACGGATCCA 
CGGTTCCACC GAGCCAAGGT GGATGCCTCT CCCGACCTTT CCGACAGCCC TGCCAGCTGC 
TCGCCCAGCA AGAGCAAACG GGCCCAAGAG GAGTGGGCCA AGAGTGAAGG CCTGATGCCG 
AGGAGCATGT CCCTCTCCAG CACTAGGTAT GGCCGATCAC GAACACCGCC CTCCGCTGCC 
AGCAGCAAGT ACAATGTTCG CAACCGCATC CAGAGCAGCC CCATGACAGT GATTAGCGAA 
GGAGATGGGG AAGTGGTGGA GCAGACAGAG AGCAGGGTCC AAAGGACTTT GGATGAGCAG 
CGAGAGAGGC AGCTCGATGT ATTTAACAGT GATGAAATAG ACATGAATGA CTTTTCGTAT 
TCTGAGGAGC TGGACTGCAA GGAGACCTTG GATCCAACCC ATCTGGACTT AATGACTCAG 
CCGGGTACTT CGAGAAAGTA CCTAAGTCCT TCAGCTGAAG AAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004935744.1
CDS	16..5103
Translation	MIAYVRCLPAEPMVGCLSSSKWQPGCKDEDEEELIPLHAGRMGNAYPKLPGRLKRKLLRKEDDSFWPTVSNLDEESRWTVHYTAPWHQQENVFLPSSRPPCVEDLHRQAKLNLKSVLRECDKLRRDGYRSSQYYSQGPTFSSSSSAICGSYQDDYEEIEQKCSVSSPEEEKLITIKRPKTPVSNDLSDINTQTNWTKSLPLPTPEEKMRQQAQAVQTDVVPINVTGENFDRQASIRRSLIYTDTVVRRPKKVKRRKTITGIPDNIQKELAVGTGQSDFRGHSMYVPDHSSTLGRLDSYRSAMQRSETKDTSCQTEEVKVVPPSMRRIRAQKGQGIAAQMSQFSSSSGNMSVMSDSAAVIFASRQNSDIGFHSLPRAGARVSLQSLEQTQSISRQTEDIAGTLPHQMSKLQVDDDVAHLRNNAITGTVSRPKSQEVRSCDSEKSTSPACVVSPHATYSTSVIPNATLSSSSEVIAIHTAQSTGSLDNKISSSAVYPKPRDNPVANNAISGKEDHHSSSGNWSESSSTRHSQTSDTIPSHTVMMLSLGDSAVSLSTPGNVEAGSQSVNYSCRSNLASPNQSQDSDGRSESSYSGERTQGVVSSTEHWLYKSAENSETASCKVACTTPGCATPGSNLSSSSLERTSAKEDSTSLYSVDHDGYYSSMSMDSGLKSDMPCYSTNGFGNPRDSVINVFEAKEKKHQDDQPGQGEKSLARNISLKKAKKPPLPPSRTDSLRRVPKKKAQSNGQVLDETLIATLQHSLQLNLKCKNGSSPSQSPCSDYEDPWVLRSRSQSSVSASSSMMSTTAPNLYSICTVTPSQSETSSIKSEYADQWGYYSDYAAVADDQMKSPVTHSASTSSALSDYSISHFSDGSRASVPQVPAGLAKPKSTSPEKSHRVTSPSSGYSSQSNTPTALTPVPVFLKSASSGNGKSKLKPKVPERKSSLLSSVSISSSSTSLSSNTSTEGSVNVKKLDPTPSSPPDSGAPLPPPPLLAPPPHCLELSPPSSPPPPPAEAVDISPLPASPTFPPPPPEADVNSSFAHSVQWLPQEASIGSYSSPVPPPVFPLVVPPPAPPLDPKLTKGAMICPQYSFKKHDQEDSCYNAVKQPFSKQDASRPVMPLVTTKALQMVQLRSIRKATEGDPSPESASETISQEKGIVTSSSQSSLRPSLSLRLSNSLGEEEMKIHGTSFKNTPQTLAQSSPLVLSDNVPMLESDQKAAVTMGLSKSLEPDSPGSAPEATPKSPVRSEDFHSGMPLQGSPASPDKTQVVLPNKKPPPISKKPKLFLVVPPPQLDLTVEKIAEVSDTVRSTSSPTKRDAVLTHCEEARNCLTDGLSSSEMDSGSLVPEGGAARVTFSEAVGVGAFVVQSAASPVQEEPRQEEQSVFDEGSSSGSSQDSSSNTEGHPSQENESAEVFESETANSSFLPSNNYGEETDGVATPARPRTTEDLFAAIHRSKRKVLGRKDSEDDRTRNHSPSPPVTPTGASPTLSTLKQAGSIQRSVRKSSTSNDNFKALLLKKGSRCDTSSRMSAAEMLKHTDPRFHRAKVDASPDLSDSPASCSPSKSKRAQEEWAKSEGLMPRSMSLSSTRYGRSRTPPSAASSKYNVRNRIQSSPMTVISEGDGEVVEQTESRVQRTLDEQRERQLDVFNSDEIDMNDFSYSEELDCKETLDPTHLDLMTQPGTSRKYLSPSAEES

Target ORF information:

RefSeq Version	XM_004935687.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NHS-like 1 (NHSL1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004935687.2

ORF Insert Sequence:

ATGATCGCCT ATGTGCGCTG CCTGCCTGCA GAGCCGATGG TCGGGTGCCT CAGCAGCAGC 
AAGTGGCAGC CGGGCTGCAA GGATGAGGAT GAGGAGGAAC TCATCCCACT GCATGCTGGA 
AGGATGGGCA ACGCGTACCC CAAGTTGCCC GGGCGCCTCA AGAGGAAGCT GCTGAGAAAG 
GAGGATGACA GCTTCTGGCC GACTGTTTCC AACTTAGATG AAGAGAGCAG ATGGACCGTC 
CATTACACTG CTCCATGGCA CCAGCAGGAA AATGTGTTCC TGCCTTCCTC AAGGCCGCCT 
TGTGTGGAGG ACTTGCACCG GCAAGCAAAG CTGAACCTCA AGTCAGTACT GAGAGAGTGT 
GACAAGCTGC GGAGAGATGG CTATCGGAGC TCTCAGTATT ACTCTCAGGG CCCCACCTTC 
TCCTCATCCT CCAGTGCAAT CTGTGGAAGT TACCAGGACG ATTATGAAGA AATTGAACAA 
AAGTGTTCTG TCTCTTCCCC AGAAGAAGAA AAGCTTATCA CCATCAAAAG GCCTAAAACC 
CCAGTTTCAA ATGACTTATC AGATATCAAC ACACAAACCA ACTGGACTAA GTCACTTCCA 
CTGCCAACAC CAGAAGAGAA GATGCGACAG CAAGCACAGG CAGTCCAAAC AGATGTGGTT 
CCTATCAATG TGACTGGGGA GAATTTCGAC CGCCAAGCCA GCATTCGGCG GTCTCTAATT 
TACACAGACA CGGTGGTAAG ACGGCCGAAG AAAGTCAAAA GGAGAAAGAC TATAACAGGA 
ATCCCTGACA ACATACAAAA GGAGCTAGCA GTTGGCACAG GCCAAAGTGA TTTCCGAGGG 
CACTCGATGT ATGTCCCAGA TCACAGTTCC ACGCTAGGGC GACTAGACAG CTATCGCTCT 
GCTATGCAGC GGTCTGAAAC CAAGGACACC AGCTGCCAGA CTGAAGAAGT TAAAGTTGTG 
CCTCCTTCGA TGAGAAGAAT ACGAGCACAG AAAGGACAAG GCATTGCAGC TCAGATGTCT 
CAGTTCTCCA GCTCATCTGG AAACATGTCT GTGATGAGTG ATTCTGCTGC AGTTATATTT 
GCTTCTCGCC AGAATAGTGA CATAGGTTTT CACAGCTTGC CTCGGGCTGG TGCAAGAGTG 
TCTCTGCAGT CCTTAGAACA GACCCAGAGC ATCTCCAGAC AGACAGAAGA CATTGCTGGT 
ACTTTGCCTC ACCAGATGAG TAAACTGCAA GTGGATGATG ATGTTGCACA TCTGAGAAAT 
AATGCCATAA CAGGAACTGT TTCGAGGCCA AAGTCTCAAG AGGTAAGAAG TTGTGACAGT 
GAAAAGTCCA CTAGCCCAGC ATGTGTGGTC TCTCCTCATG CCACCTACTC AACAAGCGTC 
ATACCCAATG CGACACTGTC ATCCTCCTCA GAAGTTATTG CTATTCACAC TGCCCAGAGT 
ACTGGATCGC TGGATAACAA AATTAGCAGT TCTGCTGTGT ACCCAAAACC AAGAGATAAT 
CCTGTTGCCA ACAATGCAAT AAGTGGGAAA GAAGATCACC ATTCTTCAAG TGGTAACTGG 
AGTGAGAGTA GCTCCACACG TCACTCACAG ACTTCAGATA CCATCCCATC TCATACTGTC 
ATGATGCTTT CTCTTGGTGA CTCTGCTGTC TCTCTCAGCA CTCCTGGAAA TGTAGAGGCT 
GGCTCTCAGA GTGTGAACTA CAGCTGCAGA AGCAATCTTG CTTCACCAAA CCAGTCCCAG 
GACAGCGATG GTAGGAGTGA ATCTAGCTAC TCAGGGGAGC GAACACAAGG TGTAGTGAGC 
AGCACAGAGC ACTGGCTATA CAAGTCTGCG GAAAATAGTG AGACTGCATC ATGCAAGGTA 
GCTTGTACCA CACCAGGCTG TGCCACTCCT GGTAGCAACC TGAGCAGCAG CAGCCTGGAG 
AGGACATCAG CCAAAGAAGA TTCCACTTCA CTGTATTCTG TAGACCACGA TGGTTATTAC 
AGCTCTATGA GCATGGACTC TGGACTGAAG TCAGACATGC CATGCTATAG TACTAATGGT 
TTTGGGAACC CCAGAGACAG CGTGATTAAT GTCTTTGAAG CAAAGGAGAA GAAACATCAA 
GATGACCAGC CAGGCCAAGG TGAAAAATCC CTTGCAAGAA ACATCTCTCT AAAAAAGGCA 
AAGAAGCCGC CTTTACCTCC GTCTAGAACA GACTCACTCA GAAGGGTACC CAAGAAGAAA 
GCTCAGTCCA ATGGACAGGT ACTTGATGAA ACCCTCATTG CTACCCTCCA GCATTCTCTG 
CAGTTGAATC TCAAGTGCAA AAATGGCAGC TCCCCTTCCC AGAGCCCCTG CAGTGACTAT 
GAGGATCCCT GGGTGTTGCG CTCTCGCAGC CAAAGCTCGG TCAGCGCAAG CAGCAGCATG 
ATGTCCACTA CTGCCCCAAA CCTGTACTCC ATTTGCACAG TCACTCCCTC TCAGAGTGAA 
ACAAGCAGCA TCAAGTCAGA GTATGCTGAC CAGTGGGGTT ACTACAGTGA CTATGCTGCA 
GTGGCAGATG ACCAGATGAA GTCTCCAGTG ACCCATTCTG CTAGTACCTC TTCTGCTCTC 
AGTGATTACA GCATCAGCCA CTTCAGTGAT GGCTCAAGGG CTTCTGTACC ACAAGTGCCC 
GCTGGACTGG CCAAACCAAA GAGCACTTCT CCAGAGAAGT CCCACCGAGT CACATCGCCA 
TCCAGTGGGT ACTCCAGCCA GTCCAATACA CCCACTGCAC TCACTCCAGT GCCTGTGTTT 
TTAAAATCTG CGTCATCAGG AAATGGGAAA TCCAAGCTGA AACCTAAAGT GCCTGAAAGG 
AAATCTTCTC TTCTGTCTTC AGTCTCCATT TCTTCATCCT CTACTTCTCT TTCTTCAAAT 
ACATCTACTG AAGGGAGTGT GAATGTGAAG AAATTGGACC CCACCCCGAG TTCTCCTCCA 
GATTCTGGTG CACCTCTTCC TCCGCCTCCT CTTCTAGCAC CTCCTCCACA TTGCCTTGAG 
CTCTCTCCTC CCTCTTCCCC TCCTCCTCCT CCAGCAGAAG CCGTGGATAT ATCACCATTG 
CCAGCTTCTC CCACATTCCC CCCCCCTCCA CCAGAAGCTG ATGTAAATTC TTCCTTTGCT 
CACAGTGTCC AGTGGTTACC ACAAGAAGCT TCCATCGGTT CGTACTCTTC CCCTGTTCCT 
CCTCCTGTTT TCCCTTTAGT TGTCCCACCA CCAGCCCCAC CTCTTGATCC CAAACTGACA 
AAAGGTGCAA TGATATGCCC ACAGTATTCA TTTAAGAAAC ATGATCAGGA AGATTCTTGC 
TACAATGCTG TGAAACAGCC ATTCAGCAAG CAAGATGCAT CAAGACCTGT GATGCCCTTG 
GTAACCACCA AAGCATTGCA AATGGTGCAG CTGAGGTCTA TCAGAAAAGC AACAGAAGGT 
GACCCATCAC CTGAATCTGC TTCTGAAACC ATCTCTCAGG AAAAGGGTAT TGTCACTTCA 
TCATCACAGT CTTCCCTAAG GCCATCTCTC TCACTACGAC TCAGTAACAG CTTAGGTGAA 
GAGGAGATGA AGATTCATGG CACTTCATTT AAAAACACAC CTCAAACACT GGCTCAGAGT 
TCTCCTCTGG TACTCTCTGA CAACGTACCC ATGCTTGAGA GCGATCAAAA AGCTGCAGTT 
ACTATGGGTC TAAGCAAGTC TTTGGAACCA GATTCCCCAG GGTCAGCTCC AGAAGCCACA 
CCCAAGTCCC CAGTTCGGAG CGAAGACTTC CATTCTGGCA TGCCACTGCA GGGGTCACCA 
GCATCTCCAG ACAAGACTCA GGTCGTTTTG CCAAACAAGA AACCACCTCC CATTTCAAAG 
AAACCCAAAC TGTTCCTTGT TGTACCACCT CCACAGTTAG ATCTCACAGT GGAGAAAATA 
GCTGAAGTGA GTGATACTGT CAGAAGCACA TCAAGCCCAA CTAAGAGAGA CGCTGTGCTT 
ACACACTGCG AGGAGGCGAG AAATTGCCTT ACAGATGGAC TCAGCTCCAG CGAGATGGAC 
TCTGGCAGCC TGGTTCCTGA GGGAGGAGCT GCCAGAGTCA CCTTCTCTGA AGCAGTGGGA 
GTTGGTGCCT TTGTGGTACA GTCCGCTGCA TCACCAGTTC AAGAGGAGCC CAGGCAAGAG 
GAGCAGTCAG TTTTTGATGA AGGAAGCAGC TCAGGCAGCA GCCAAGACAG CAGCAGTAAC 
ACTGAAGGAC ACCCGTCTCA GGAGAACGAA AGTGCCGAGG TGTTTGAATC AGAAACAGCA 
AATAGTTCAT TCCTGCCAAG CAATAATTAT GGGGAAGAGA CGGATGGAGT GGCAACACCA 
GCGAGACCGA GGACTACTGA GGATCTTTTT GCAGCCATTC ACAGATCCAA AAGGAAAGTC 
CTTGGCCGTA AGGATTCTGA GGACGATCGT ACCCGCAACC ATTCTCCTTC ACCCCCCGTA 
ACACCCACTG GTGCTTCCCC AACCTTATCT ACCCTCAAAC AGGCAGGATC TATTCAGAGA 
AGCGTTCGCA AGAGCAGCAC CAGCAATGAC AACTTCAAAG CCCTGCTGCT GAAGAAAGGG 
AGTCGCTGTG ACACCAGTTC CCGCATGTCA GCAGCAGAGA TGTTGAAGCA CACGGATCCA 
CGGTTCCACC GAGCCAAGGT GGATGCCTCT CCCGACCTTT CCGACAGCCC TGCCAGCTGC 
TCGCCCAGCA AGAGCAAACG GGCCCAAGAG GAGTGGGCCA AGAGTGAAGG CCTGATGCCG 
AGGAGCATGT CCCTCTCCAG CACTAGGTAT GGCCGATCAC GAACACCGCC CTCCGCTGCC 
AGCAGCAAGT ACAATGTTCG CAACCGCATC CAGAGCAGCC CCATGACAGT GATTAGCGAA 
GGAGATGGGG AAGTGGTGGA GCAGACAGAG AGCAGGGTCC AAAGGACTTT GGATGAGCAG 
CGAGAGAGGC AGCTCGATGT ATTTAACAGT GATGAAATAG ACATGAATGA CTTTTCGTAT 
TCTGAGGAGC TGGACTGCAA GGAGACCTTG GATCCAACCC ATCTGGACTT AATGACTCAG 
CCGGGTACTT CGAGAAAGTA CCTAAGTCCT TCAGCTGAAG AAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_004935687.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004935744.1 Gallus gallus NHS like 1 (NHSL1), transcript variant X8, mRNA.

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CloneID	OGa06404
Clone ID Related Accession (Same CDS sequence)	XM_004935687.2 , XM_004935687.3
Accession Version	XM_004935687.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	NHS-like protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004935687.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCGCCT ATGTGCGCTG CCTGCCTGCA GAGCCGATGG TCGGGTGCCT CAGCAGCAGC 
AAGTGGCAGC CGGGCTGCAA GGATGAGGAT GAGGAGGAAC TCATCCCACT GCATGCTGGA 
AGGATGGGCA ACGCGTACCC CAAGTTGCCC GGGCGCCTCA AGAGGAAGCT GCTGAGAAAG 
GAGGATGACA GCTTCTGGCC GACTGTTTCC AACTTAGATG AAGAGAGCAG ATGGACCGTC 
CATTACACTG CTCCATGGCA CCAGCAGGAA AATGTGTTCC TGCCTTCCTC AAGGCCGCCT 
TGTGTGGAGG ACTTGCACCG GCAAGCAAAG CTGAACCTCA AGTCAGTACT GAGAGAGTGT 
GACAAGCTGC GGAGAGATGG CTATCGGAGC TCTCAGTATT ACTCTCAGGG CCCCACCTTC 
TCCTCATCCT CCAGTGCAAT CTGTGGAAGT TACCAGGACG ATTATGAAGA AATTGAACAA 
AAGTGTTCTG TCTCTTCCCC AGAAGAAGAA AAGCTTATCA CCATCAAAAG GCCTAAAACC 
CCAGTTTCAA ATGACTTATC AGATATCAAC ACACAAACCA ACTGGACTAA GTCACTTCCA 
CTGCCAACAC CAGAAGAGAA GATGCGACAG CAAGCACAGG CAGTCCAAAC AGATGTGGTT 
CCTATCAATG TGACTGGGGA GAATTTCGAC CGCCAAGCCA GCATTCGGCG GTCTCTAATT 
TACACAGACA CGGTGGTAAG ACGGCCGAAG AAAGTCAAAA GGAGAAAGAC TATAACAGGA 
ATCCCTGACA ACATACAAAA GGAGCTAGCA GTTGGCACAG GCCAAAGTGA TTTCCGAGGG 
CACTCGATGT ATGTCCCAGA TCACAGTTCC ACGCTAGGGC GACTAGACAG CTATCGCTCT 
GCTATGCAGC GGTCTGAAAC CAAGGACACC AGCTGCCAGA CTGAAGAAGT TAAAGTTGTG 
CCTCCTTCGA TGAGAAGAAT ACGAGCACAG AAAGGACAAG GCATTGCAGC TCAGATGTCT 
CAGTTCTCCA GCTCATCTGG AAACATGTCT GTGATGAGTG ATTCTGCTGC AGTTATATTT 
GCTTCTCGCC AGAATAGTGA CATAGGTTTT CACAGCTTGC CTCGGGCTGG TGCAAGAGTG 
TCTCTGCAGT CCTTAGAACA GACCCAGAGC ATCTCCAGAC AGACAGAAGA CATTGCTGGT 
ACTTTGCCTC ACCAGATGAG TAAACTGCAA GTGGATGATG ATGTTGCACA TCTGAGAAAT 
AATGCCATAA CAGGAACTGT TTCGAGGCCA AAGTCTCAAG AGGTAAGAAG TTGTGACAGT 
GAAAAGTCCA CTAGCCCAGC ATGTGTGGTC TCTCCTCATG CCACCTACTC AACAAGCGTC 
ATACCCAATG CGACACTGTC ATCCTCCTCA GAAGTTATTG CTATTCACAC TGCCCAGAGT 
ACTGGATCGC TGGATAACAA AATTAGCAGT TCTGCTGTGT ACCCAAAACC AAGAGATAAT 
CCTGTTGCCA ACAATGCAAT AAGTGGGAAA GAAGATCACC ATTCTTCAAG TGGTAACTGG 
AGTGAGAGTA GCTCCACACG TCACTCACAG ACTTCAGATA CCATCCCATC TCATACTGTC 
ATGATGCTTT CTCTTGGTGA CTCTGCTGTC TCTCTCAGCA CTCCTGGAAA TGTAGAGGCT 
GGCTCTCAGA GTGTGAACTA CAGCTGCAGA AGCAATCTTG CTTCACCAAA CCAGTCCCAG 
GACAGCGATG GTAGGAGTGA ATCTAGCTAC TCAGGGGAGC GAACACAAGG TGTAGTGAGC 
AGCACAGAGC ACTGGCTATA CAAGTCTGCG GAAAATAGTG AGACTGCATC ATGCAAGGTA 
GCTTGTACCA CACCAGGCTG TGCCACTCCT GGTAGCAACC TGAGCAGCAG CAGCCTGGAG 
AGGACATCAG CCAAAGAAGA TTCCACTTCA CTGTATTCTG TAGACCACGA TGGTTATTAC 
AGCTCTATGA GCATGGACTC TGGACTGAAG TCAGACATGC CATGCTATAG TACTAATGGT 
TTTGGGAACC CCAGAGACAG CGTGATTAAT GTCTTTGAAG CAAAGGAGAA GAAACATCAA 
GATGACCAGC CAGGCCAAGG TGAAAAATCC CTTGCAAGAA ACATCTCTCT AAAAAAGGCA 
AAGAAGCCGC CTTTACCTCC GTCTAGAACA GACTCACTCA GAAGGGTACC CAAGAAGAAA 
GCTCAGTCCA ATGGACAGGT ACTTGATGAA ACCCTCATTG CTACCCTCCA GCATTCTCTG 
CAGTTGAATC TCAAGTGCAA AAATGGCAGC TCCCCTTCCC AGAGCCCCTG CAGTGACTAT 
GAGGATCCCT GGGTGTTGCG CTCTCGCAGC CAAAGCTCGG TCAGCGCAAG CAGCAGCATG 
ATGTCCACTA CTGCCCCAAA CCTGTACTCC ATTTGCACAG TCACTCCCTC TCAGAGTGAA 
ACAAGCAGCA TCAAGTCAGA GTATGCTGAC CAGTGGGGTT ACTACAGTGA CTATGCTGCA 
GTGGCAGATG ACCAGATGAA GTCTCCAGTG ACCCATTCTG CTAGTACCTC TTCTGCTCTC 
AGTGATTACA GCATCAGCCA CTTCAGTGAT GGCTCAAGGG CTTCTGTACC ACAAGTGCCC 
GCTGGACTGG CCAAACCAAA GAGCACTTCT CCAGAGAAGT CCCACCGAGT CACATCGCCA 
TCCAGTGGGT ACTCCAGCCA GTCCAATACA CCCACTGCAC TCACTCCAGT GCCTGTGTTT 
TTAAAATCTG CGTCATCAGG AAATGGGAAA TCCAAGCTGA AACCTAAAGT GCCTGAAAGG 
AAATCTTCTC TTCTGTCTTC AGTCTCCATT TCTTCATCCT CTACTTCTCT TTCTTCAAAT 
ACATCTACTG AAGGGAGTGT GAATGTGAAG AAATTGGACC CCACCCCGAG TTCTCCTCCA 
GATTCTGGTG CACCTCTTCC TCCGCCTCCT CTTCTAGCAC CTCCTCCACA TTGCCTTGAG 
CTCTCTCCTC CCTCTTCCCC TCCTCCTCCT CCAGCAGAAG CCGTGGATAT ATCACCATTG 
CCAGCTTCTC CCACATTCCC CCCCCCTCCA CCAGAAGCTG ATGTAAATTC TTCCTTTGCT 
CACAGTGTCC AGTGGTTACC ACAAGAAGCT TCCATCGGTT CGTACTCTTC CCCTGTTCCT 
CCTCCTGTTT TCCCTTTAGT TGTCCCACCA CCAGCCCCAC CTCTTGATCC CAAACTGACA 
AAAGGTGCAA TGATATGCCC ACAGTATTCA TTTAAGAAAC ATGATCAGGA AGATTCTTGC 
TACAATGCTG TGAAACAGCC ATTCAGCAAG CAAGATGCAT CAAGACCTGT GATGCCCTTG 
GTAACCACCA AAGCATTGCA AATGGTGCAG CTGAGGTCTA TCAGAAAAGC AACAGAAGGT 
GACCCATCAC CTGAATCTGC TTCTGAAACC ATCTCTCAGG AAAAGGGTAT TGTCACTTCA 
TCATCACAGT CTTCCCTAAG GCCATCTCTC TCACTACGAC TCAGTAACAG CTTAGGTGAA 
GAGGAGATGA AGATTCATGG CACTTCATTT AAAAACACAC CTCAAACACT GGCTCAGAGT 
TCTCCTCTGG TACTCTCTGA CAACGTACCC ATGCTTGAGA GCGATCAAAA AGCTGCAGTT 
ACTATGGGTC TAAGCAAGTC TTTGGAACCA GATTCCCCAG GGTCAGCTCC AGAAGCCACA 
CCCAAGTCCC CAGTTCGGAG CGAAGACTTC CATTCTGGCA TGCCACTGCA GGGGTCACCA 
GCATCTCCAG ACAAGACTCA GGTCGTTTTG CCAAACAAGA AACCACCTCC CATTTCAAAG 
AAACCCAAAC TGTTCCTTGT TGTACCACCT CCACAGTTAG ATCTCACAGT GGAGAAAATA 
GCTGAAGTGA GTGATACTGT CAGAAGCACA TCAAGCCCAA CTAAGAGAGA CGCTGTGCTT 
ACACACTGCG AGGAGGCGAG AAATTGCCTT ACAGATGGAC TCAGCTCCAG CGAGATGGAC 
TCTGGCAGCC TGGTTCCTGA GGGAGGAGCT GCCAGAGTCA CCTTCTCTGA AGCAGTGGGA 
GTTGGTGCCT TTGTGGTACA GTCCGCTGCA TCACCAGTTC AAGAGGAGCC CAGGCAAGAG 
GAGCAGTCAG TTTTTGATGA AGGAAGCAGC TCAGGCAGCA GCCAAGACAG CAGCAGTAAC 
ACTGAAGGAC ACCCGTCTCA GGAGAACGAA AGTGCCGAGG TGTTTGAATC AGAAACAGCA 
AATAGTTCAT TCCTGCCAAG CAATAATTAT GGGGAAGAGA CGGATGGAGT GGCAACACCA 
GCGAGACCGA GGACTACTGA GGATCTTTTT GCAGCCATTC ACAGATCCAA AAGGAAAGTC 
CTTGGCCGTA AGGATTCTGA GGACGATCGT ACCCGCAACC ATTCTCCTTC ACCCCCCGTA 
ACACCCACTG GTGCTTCCCC AACCTTATCT ACCCTCAAAC AGGCAGGATC TATTCAGAGA 
AGCGTTCGCA AGAGCAGCAC CAGCAATGAC AACTTCAAAG CCCTGCTGCT GAAGAAAGGG 
AGTCGCTGTG ACACCAGTTC CCGCATGTCA GCAGCAGAGA TGTTGAAGCA CACGGATCCA 
CGGTTCCACC GAGCCAAGGT GGATGCCTCT CCCGACCTTT CCGACAGCCC TGCCAGCTGC 
TCGCCCAGCA AGAGCAAACG GGCCCAAGAG GAGTGGGCCA AGAGTGAAGG CCTGATGCCG 
AGGAGCATGT CCCTCTCCAG CACTAGGTAT GGCCGATCAC GAACACCGCC CTCCGCTGCC 
AGCAGCAAGT ACAATGTTCG CAACCGCATC CAGAGCAGCC CCATGACAGT GATTAGCGAA 
GGAGATGGGG AAGTGGTGGA GCAGACAGAG AGCAGGGTCC AAAGGACTTT GGATGAGCAG 
CGAGAGAGGC AGCTCGATGT ATTTAACAGT GATGAAATAG ACATGAATGA CTTTTCGTAT 
TCTGAGGAGC TGGACTGCAA GGAGACCTTG GATCCAACCC ATCTGGACTT AATGACTCAG 
CCGGGTACTT CGAGAAAGTA CCTAAGTCCT TCAGCTGAAG AAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004935744.1
CDS	16..5103
Translation	MIAYVRCLPAEPMVGCLSSSKWQPGCKDEDEEELIPLHAGRMGNAYPKLPGRLKRKLLRKEDDSFWPTVSNLDEESRWTVHYTAPWHQQENVFLPSSRPPCVEDLHRQAKLNLKSVLRECDKLRRDGYRSSQYYSQGPTFSSSSSAICGSYQDDYEEIEQKCSVSSPEEEKLITIKRPKTPVSNDLSDINTQTNWTKSLPLPTPEEKMRQQAQAVQTDVVPINVTGENFDRQASIRRSLIYTDTVVRRPKKVKRRKTITGIPDNIQKELAVGTGQSDFRGHSMYVPDHSSTLGRLDSYRSAMQRSETKDTSCQTEEVKVVPPSMRRIRAQKGQGIAAQMSQFSSSSGNMSVMSDSAAVIFASRQNSDIGFHSLPRAGARVSLQSLEQTQSISRQTEDIAGTLPHQMSKLQVDDDVAHLRNNAITGTVSRPKSQEVRSCDSEKSTSPACVVSPHATYSTSVIPNATLSSSSEVIAIHTAQSTGSLDNKISSSAVYPKPRDNPVANNAISGKEDHHSSSGNWSESSSTRHSQTSDTIPSHTVMMLSLGDSAVSLSTPGNVEAGSQSVNYSCRSNLASPNQSQDSDGRSESSYSGERTQGVVSSTEHWLYKSAENSETASCKVACTTPGCATPGSNLSSSSLERTSAKEDSTSLYSVDHDGYYSSMSMDSGLKSDMPCYSTNGFGNPRDSVINVFEAKEKKHQDDQPGQGEKSLARNISLKKAKKPPLPPSRTDSLRRVPKKKAQSNGQVLDETLIATLQHSLQLNLKCKNGSSPSQSPCSDYEDPWVLRSRSQSSVSASSSMMSTTAPNLYSICTVTPSQSETSSIKSEYADQWGYYSDYAAVADDQMKSPVTHSASTSSALSDYSISHFSDGSRASVPQVPAGLAKPKSTSPEKSHRVTSPSSGYSSQSNTPTALTPVPVFLKSASSGNGKSKLKPKVPERKSSLLSSVSISSSSTSLSSNTSTEGSVNVKKLDPTPSSPPDSGAPLPPPPLLAPPPHCLELSPPSSPPPPPAEAVDISPLPASPTFPPPPPEADVNSSFAHSVQWLPQEASIGSYSSPVPPPVFPLVVPPPAPPLDPKLTKGAMICPQYSFKKHDQEDSCYNAVKQPFSKQDASRPVMPLVTTKALQMVQLRSIRKATEGDPSPESASETISQEKGIVTSSSQSSLRPSLSLRLSNSLGEEEMKIHGTSFKNTPQTLAQSSPLVLSDNVPMLESDQKAAVTMGLSKSLEPDSPGSAPEATPKSPVRSEDFHSGMPLQGSPASPDKTQVVLPNKKPPPISKKPKLFLVVPPPQLDLTVEKIAEVSDTVRSTSSPTKRDAVLTHCEEARNCLTDGLSSSEMDSGSLVPEGGAARVTFSEAVGVGAFVVQSAASPVQEEPRQEEQSVFDEGSSSGSSQDSSSNTEGHPSQENESAEVFESETANSSFLPSNNYGEETDGVATPARPRTTEDLFAAIHRSKRKVLGRKDSEDDRTRNHSPSPPVTPTGASPTLSTLKQAGSIQRSVRKSSTSNDNFKALLLKKGSRCDTSSRMSAAEMLKHTDPRFHRAKVDASPDLSDSPASCSPSKSKRAQEEWAKSEGLMPRSMSLSSTRYGRSRTPPSAASSKYNVRNRIQSSPMTVISEGDGEVVEQTESRVQRTLDEQRERQLDVFNSDEIDMNDFSYSEELDCKETLDPTHLDLMTQPGTSRKYLSPSAEES

Target ORF information:

RefSeq Version	XM_004935687.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NHS like 1 (NHSL1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004935687.3

ORF Insert Sequence:

ATGATCGCCT ATGTGCGCTG CCTGCCTGCA GAGCCGATGG TCGGGTGCCT CAGCAGCAGC 
AAGTGGCAGC CGGGCTGCAA GGATGAGGAT GAGGAGGAAC TCATCCCACT GCATGCTGGA 
AGGATGGGCA ACGCGTACCC CAAGTTGCCC GGGCGCCTCA AGAGGAAGCT GCTGAGAAAG 
GAGGATGACA GCTTCTGGCC GACTGTTTCC AACTTAGATG AAGAGAGCAG ATGGACCGTC 
CATTACACTG CTCCATGGCA CCAGCAGGAA AATGTGTTCC TGCCTTCCTC AAGGCCGCCT 
TGTGTGGAGG ACTTGCACCG GCAAGCAAAG CTGAACCTCA AGTCAGTACT GAGAGAGTGT 
GACAAGCTGC GGAGAGATGG CTATCGGAGC TCTCAGTATT ACTCTCAGGG CCCCACCTTC 
TCCTCATCCT CCAGTGCAAT CTGTGGAAGT TACCAGGACG ATTATGAAGA AATTGAACAA 
AAGTGTTCTG TCTCTTCCCC AGAAGAAGAA AAGCTTATCA CCATCAAAAG GCCTAAAACC 
CCAGTTTCAA ATGACTTATC AGATATCAAC ACACAAACCA ACTGGACTAA GTCACTTCCA 
CTGCCAACAC CAGAAGAGAA GATGCGACAG CAAGCACAGG CAGTCCAAAC AGATGTGGTT 
CCTATCAATG TGACTGGGGA GAATTTCGAC CGCCAAGCCA GCATTCGGCG GTCTCTAATT 
TACACAGACA CGGTGGTAAG ACGGCCGAAG AAAGTCAAAA GGAGAAAGAC TATAACAGGA 
ATCCCTGACA ACATACAAAA GGAGCTAGCA GTTGGCACAG GCCAAAGTGA TTTCCGAGGG 
CACTCGATGT ATGTCCCAGA TCACAGTTCC ACGCTAGGGC GACTAGACAG CTATCGCTCT 
GCTATGCAGC GGTCTGAAAC CAAGGACACC AGCTGCCAGA CTGAAGAAGT TAAAGTTGTG 
CCTCCTTCGA TGAGAAGAAT ACGAGCACAG AAAGGACAAG GCATTGCAGC TCAGATGTCT 
CAGTTCTCCA GCTCATCTGG AAACATGTCT GTGATGAGTG ATTCTGCTGC AGTTATATTT 
GCTTCTCGCC AGAATAGTGA CATAGGTTTT CACAGCTTGC CTCGGGCTGG TGCAAGAGTG 
TCTCTGCAGT CCTTAGAACA GACCCAGAGC ATCTCCAGAC AGACAGAAGA CATTGCTGGT 
ACTTTGCCTC ACCAGATGAG TAAACTGCAA GTGGATGATG ATGTTGCACA TCTGAGAAAT 
AATGCCATAA CAGGAACTGT TTCGAGGCCA AAGTCTCAAG AGGTAAGAAG TTGTGACAGT 
GAAAAGTCCA CTAGCCCAGC ATGTGTGGTC TCTCCTCATG CCACCTACTC AACAAGCGTC 
ATACCCAATG CGACACTGTC ATCCTCCTCA GAAGTTATTG CTATTCACAC TGCCCAGAGT 
ACTGGATCGC TGGATAACAA AATTAGCAGT TCTGCTGTGT ACCCAAAACC AAGAGATAAT 
CCTGTTGCCA ACAATGCAAT AAGTGGGAAA GAAGATCACC ATTCTTCAAG TGGTAACTGG 
AGTGAGAGTA GCTCCACACG TCACTCACAG ACTTCAGATA CCATCCCATC TCATACTGTC 
ATGATGCTTT CTCTTGGTGA CTCTGCTGTC TCTCTCAGCA CTCCTGGAAA TGTAGAGGCT 
GGCTCTCAGA GTGTGAACTA CAGCTGCAGA AGCAATCTTG CTTCACCAAA CCAGTCCCAG 
GACAGCGATG GTAGGAGTGA ATCTAGCTAC TCAGGGGAGC GAACACAAGG TGTAGTGAGC 
AGCACAGAGC ACTGGCTATA CAAGTCTGCG GAAAATAGTG AGACTGCATC ATGCAAGGTA 
GCTTGTACCA CACCAGGCTG TGCCACTCCT GGTAGCAACC TGAGCAGCAG CAGCCTGGAG 
AGGACATCAG CCAAAGAAGA TTCCACTTCA CTGTATTCTG TAGACCACGA TGGTTATTAC 
AGCTCTATGA GCATGGACTC TGGACTGAAG TCAGACATGC CATGCTATAG TACTAATGGT 
TTTGGGAACC CCAGAGACAG CGTGATTAAT GTCTTTGAAG CAAAGGAGAA GAAACATCAA 
GATGACCAGC CAGGCCAAGG TGAAAAATCC CTTGCAAGAA ACATCTCTCT AAAAAAGGCA 
AAGAAGCCGC CTTTACCTCC GTCTAGAACA GACTCACTCA GAAGGGTACC CAAGAAGAAA 
GCTCAGTCCA ATGGACAGGT ACTTGATGAA ACCCTCATTG CTACCCTCCA GCATTCTCTG 
CAGTTGAATC TCAAGTGCAA AAATGGCAGC TCCCCTTCCC AGAGCCCCTG CAGTGACTAT 
GAGGATCCCT GGGTGTTGCG CTCTCGCAGC CAAAGCTCGG TCAGCGCAAG CAGCAGCATG 
ATGTCCACTA CTGCCCCAAA CCTGTACTCC ATTTGCACAG TCACTCCCTC TCAGAGTGAA 
ACAAGCAGCA TCAAGTCAGA GTATGCTGAC CAGTGGGGTT ACTACAGTGA CTATGCTGCA 
GTGGCAGATG ACCAGATGAA GTCTCCAGTG ACCCATTCTG CTAGTACCTC TTCTGCTCTC 
AGTGATTACA GCATCAGCCA CTTCAGTGAT GGCTCAAGGG CTTCTGTACC ACAAGTGCCC 
GCTGGACTGG CCAAACCAAA GAGCACTTCT CCAGAGAAGT CCCACCGAGT CACATCGCCA 
TCCAGTGGGT ACTCCAGCCA GTCCAATACA CCCACTGCAC TCACTCCAGT GCCTGTGTTT 
TTAAAATCTG CGTCATCAGG AAATGGGAAA TCCAAGCTGA AACCTAAAGT GCCTGAAAGG 
AAATCTTCTC TTCTGTCTTC AGTCTCCATT TCTTCATCCT CTACTTCTCT TTCTTCAAAT 
ACATCTACTG AAGGGAGTGT GAATGTGAAG AAATTGGACC CCACCCCGAG TTCTCCTCCA 
GATTCTGGTG CACCTCTTCC TCCGCCTCCT CTTCTAGCAC CTCCTCCACA TTGCCTTGAG 
CTCTCTCCTC CCTCTTCCCC TCCTCCTCCT CCAGCAGAAG CCGTGGATAT ATCACCATTG 
CCAGCTTCTC CCACATTCCC CCCCCCTCCA CCAGAAGCTG ATGTAAATTC TTCCTTTGCT 
CACAGTGTCC AGTGGTTACC ACAAGAAGCT TCCATCGGTT CGTACTCTTC CCCTGTTCCT 
CCTCCTGTTT TCCCTTTAGT TGTCCCACCA CCAGCCCCAC CTCTTGATCC CAAACTGACA 
AAAGGTGCAA TGATATGCCC ACAGTATTCA TTTAAGAAAC ATGATCAGGA AGATTCTTGC 
TACAATGCTG TGAAACAGCC ATTCAGCAAG CAAGATGCAT CAAGACCTGT GATGCCCTTG 
GTAACCACCA AAGCATTGCA AATGGTGCAG CTGAGGTCTA TCAGAAAAGC AACAGAAGGT 
GACCCATCAC CTGAATCTGC TTCTGAAACC ATCTCTCAGG AAAAGGGTAT TGTCACTTCA 
TCATCACAGT CTTCCCTAAG GCCATCTCTC TCACTACGAC TCAGTAACAG CTTAGGTGAA 
GAGGAGATGA AGATTCATGG CACTTCATTT AAAAACACAC CTCAAACACT GGCTCAGAGT 
TCTCCTCTGG TACTCTCTGA CAACGTACCC ATGCTTGAGA GCGATCAAAA AGCTGCAGTT 
ACTATGGGTC TAAGCAAGTC TTTGGAACCA GATTCCCCAG GGTCAGCTCC AGAAGCCACA 
CCCAAGTCCC CAGTTCGGAG CGAAGACTTC CATTCTGGCA TGCCACTGCA GGGGTCACCA 
GCATCTCCAG ACAAGACTCA GGTCGTTTTG CCAAACAAGA AACCACCTCC CATTTCAAAG 
AAACCCAAAC TGTTCCTTGT TGTACCACCT CCACAGTTAG ATCTCACAGT GGAGAAAATA 
GCTGAAGTGA GTGATACTGT CAGAAGCACA TCAAGCCCAA CTAAGAGAGA CGCTGTGCTT 
ACACACTGCG AGGAGGCGAG AAATTGCCTT ACAGATGGAC TCAGCTCCAG CGAGATGGAC 
TCTGGCAGCC TGGTTCCTGA GGGAGGAGCT GCCAGAGTCA CCTTCTCTGA AGCAGTGGGA 
GTTGGTGCCT TTGTGGTACA GTCCGCTGCA TCACCAGTTC AAGAGGAGCC CAGGCAAGAG 
GAGCAGTCAG TTTTTGATGA AGGAAGCAGC TCAGGCAGCA GCCAAGACAG CAGCAGTAAC 
ACTGAAGGAC ACCCGTCTCA GGAGAACGAA AGTGCCGAGG TGTTTGAATC AGAAACAGCA 
AATAGTTCAT TCCTGCCAAG CAATAATTAT GGGGAAGAGA CGGATGGAGT GGCAACACCA 
GCGAGACCGA GGACTACTGA GGATCTTTTT GCAGCCATTC ACAGATCCAA AAGGAAAGTC 
CTTGGCCGTA AGGATTCTGA GGACGATCGT ACCCGCAACC ATTCTCCTTC ACCCCCCGTA 
ACACCCACTG GTGCTTCCCC AACCTTATCT ACCCTCAAAC AGGCAGGATC TATTCAGAGA 
AGCGTTCGCA AGAGCAGCAC CAGCAATGAC AACTTCAAAG CCCTGCTGCT GAAGAAAGGG 
AGTCGCTGTG ACACCAGTTC CCGCATGTCA GCAGCAGAGA TGTTGAAGCA CACGGATCCA 
CGGTTCCACC GAGCCAAGGT GGATGCCTCT CCCGACCTTT CCGACAGCCC TGCCAGCTGC 
TCGCCCAGCA AGAGCAAACG GGCCCAAGAG GAGTGGGCCA AGAGTGAAGG CCTGATGCCG 
AGGAGCATGT CCCTCTCCAG CACTAGGTAT GGCCGATCAC GAACACCGCC CTCCGCTGCC 
AGCAGCAAGT ACAATGTTCG CAACCGCATC CAGAGCAGCC CCATGACAGT GATTAGCGAA 
GGAGATGGGG AAGTGGTGGA GCAGACAGAG AGCAGGGTCC AAAGGACTTT GGATGAGCAG 
CGAGAGAGGC AGCTCGATGT ATTTAACAGT GATGAAATAG ACATGAATGA CTTTTCGTAT 
TCTGAGGAGC TGGACTGCAA GGAGACCTTG GATCCAACCC ATCTGGACTT AATGACTCAG 
CCGGGTACTT CGAGAAAGTA CCTAAGTCCT TCAGCTGAAG AAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_004935690.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004935747.1 Gallus gallus NHS like 1 (NHSL1), transcript variant X9, mRNA.

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CloneID	OGa06407
Clone ID Related Accession (Same CDS sequence)	XM_004935690.3 , XM_004935690.2
Accession Version	XM_004935690.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	NHS-like protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004935690.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAGG AGTGTGTTTC GAGATCTTTC AAACTCAAAC AAAACGCAGG CTCCCTCTCG 
CGTGCTGTAA GCTGGATAAA TTTCTCCTCC TTGTCAAGGC AGACAAAGAG ATTGTTTCGC 
AGCGATGGTG AGCTCTCCTC CATTTGCGTG CAGCAGGTAG ACGAGGATGA TGAGAACTGG 
ACCTACAGGA GCCCACGCGG AAAAGCTGTT TCCAACTTAG ATGAAGAGAG CAGATGGACC 
GTCCATTACA CTGCTCCATG GCACCAGCAG GAAAATGTGT TCCTGCCTTC CTCAAGGCCG 
CCTTGTGTGG AGGACTTGCA CCGGCAAGCA AAGCTGAACC TCAAGTCAGT ACTGAGAGAG 
TGTGACAAGC TGCGGAGAGA TGGCTATCGG AGCTCTCAGT ATTACTCTCA GGGCCCCACC 
TTCTCCTCAT CCTCCAGTGC AATCTGTGGA AGTTACCAGG ACGATTATGA AGAAATTGAA 
CAAAAGTGTT CTGTCTCTTC CCCAGAAGAA GAAAAGCTTA TCACCATCAA AAGGCCTAAA 
ACCCCAGTTT CAAATGACTT ATCAGATATC AACACACAAA CCAACTGGAC TAAGTCACTT 
CCACTGCCAA CACCAGAAGA GAAGATGCGA CAGCAAGCAC AGGCAGTCCA AACAGATGTG 
GTTCCTATCA ATGTGACTGC AGTTGGCACA GGCCAAAGTG ATTTCCGAGG GCACTCGATG 
TATGTCCCAG ATCACAGTTC CACGCTAGGG CGACTAGACA GCTATCGCTC TGCTATGCAG 
CGGTCTGAAA CCAAGGACAC CAGCTGCCAG ACTGAAGAAG TTAAAGTTGT GCCTCCTTCG 
ATGAGAAGAA TACGAGCACA GAAAGGACAA GGCATTGCAG CTCAGATGTC TCAGTTCTCC 
AGCTCATCTG GAAACATGTC TGTGATGAGT GATTCTGCTG CAGTTATATT TGCTTCTCGC 
CAGAATAGTG ACATAGGTTT TCACAGCTTG CCTCGGGCTG GTGCAAGAGT GTCTCTGCAG 
TCCTTAGAAC AGACCCAGAG CATCTCCAGA CAGACAGAAG ACATTGCTGG TACTTTGCCT 
CACCAGATGA GTAAACTGCA AGTGGATGAT GATGTTGCAC ATCTGAGAAA TAATGCCATA 
ACAGGAACTG TTTCGAGGCC AAAGTCTCAA GAGGTAAGAA GTTGTGACAG TGAAAAGTCC 
ACTAGCCCAG CATGTGTGGT CTCTCCTCAT GCCACCTACT CAACAAGCGT CATACCCAAT 
GCGACACTGT CATCCTCCTC AGAAGTTATT GCTATTCACA CTGCCCAGAG TACTGGATCG 
CTGGATAACA AAATTAGCAG TTCTGCTGTG TACCCAAAAC CAAGAGATAA TCCTGTTGCC 
AACAATGCAA TAAGTGGGAA AGAAGATCAC CATTCTTCAA GTGGTAACTG GAGTGAGAGT 
AGCTCCACAC GTCACTCACA GACTTCAGAT ACCATCCCAT CTCATACTGT CATGATGCTT 
TCTCTTGGTG ACTCTGCTGT CTCTCTCAGC ACTCCTGGAA ATGTAGAGGC TGGCTCTCAG 
AGTGTGAACT ACAGCTGCAG AAGCAATCTT GCTTCACCAA ACCAGTCCCA GGACAGCGAT 
GGTAGGAGTG AATCTAGCTA CTCAGGGGAG CGAACACAAG GTGTAGTGAG CAGCACAGAG 
CACTGGCTAT ACAAGTCTGC GGAAAATAGT GAGACTGCAT CATGCAAGGT AGCTTGTACC 
ACACCAGGCT GTGCCACTCC TGGTAGCAAC CTGAGCAGCA GCAGCCTGGA GAGGACATCA 
GCCAAAGAAG ATTCCACTTC ACTGTATTCT GTAGACCACG ATGGTTATTA CAGCTCTATG 
AGCATGGACT CTGGACTGAA GTCAGACATG CCATGCTATA GTACTAATGG TTTTGGGAAC 
CCCAGAGACA GCGTGATTAA TGTCTTTGAA GCAAAGGAGA AGAAACATCA AGATGACCAG 
CCAGGCCAAG GTGAAAAATC CCTTGCAAGA AACATCTCTC TAAAAAAGGC AAAGAAGCCG 
CCTTTACCTC CGTCTAGAAC AGACTCACTC AGAAGGGTAC CCAAGAAGAA AGCTCAGTCC 
AATGGACAGG TACTTGATGA AACCCTCATT GCTACCCTCC AGCATTCTCT GCAGTTGAAT 
CTCAAGTGCA AAAATGGCAG CTCCCCTTCC CAGAGCCCCT GCAGTGACTA TGAGGATCCC 
TGGGTGTTGC GCTCTCGCAG CCAAAGCTCG GTCAGCGCAA GCAGCAGCAT GATGTCCACT 
ACTGCCCCAA ACCTGTACTC CATTTGCACA GTCACTCCCT CTCAGAGTGA AACAAGCAGC 
ATCAAGTCAG AGTATGCTGA CCAGTGGGGT TACTACAGTG ACTATGCTGC AGTGGCAGAT 
GACCAGATGA AGTCTCCAGT GACCCATTCT GCTAGTACCT CTTCTGCTCT CAGTGATTAC 
AGCATCAGCC ACTTCAGTGA TGGCTCAAGG GCTTCTGTAC CACAAGTGCC CGCTGGACTG 
GCCAAACCAA AGAGCACTTC TCCAGAGAAG TCCCACCGAG TCACATCGCC ATCCAGTGGG 
TACTCCAGCC AGTCCAATAC ACCCACTGCA CTCACTCCAG TGCCTGTGTT TTTAAAATCT 
GCGTCATCAG GAAATGGGAA ATCCAAGCTG AAACCTAAAG TGCCTGAAAG GAAATCTTCT 
CTTCTGTCTT CAGTCTCCAT TTCTTCATCC TCTACTTCTC TTTCTTCAAA TACATCTACT 
GAAGGGAGTG TGAATGTGAA GAAATTGGAC CCCACCCCGA GTTCTCCTCC AGATTCTGGT 
GCACCTCTTC CTCCGCCTCC TCTTCTAGCA CCTCCTCCAC ATTGCCTTGA GCTCTCTCCT 
CCCTCTTCCC CTCCTCCTCC TCCAGCAGAA GCCGTGGATA TATCACCATT GCCAGCTTCT 
CCCACATTCC CCCCCCCTCC ACCAGAAGCT GATGTAAATT CTTCCTTTGC TCACAGTGTC 
CAGTGGTTAC CACAAGAAGC TTCCATCGGT TCGTACTCTT CCCCTGTTCC TCCTCCTGTT 
TTCCCTTTAG TTGTCCCACC ACCAGCCCCA CCTCTTGATC CCAAACTGAC AAAAGGTGCA 
ATGATATGCC CACAGTATTC ATTTAAGAAA CATGATCAGG AAGATTCTTG CTACAATGCT 
GTGAAACAGC CATTCAGCAA GCAAGATGCA TCAAGACCTG TGATGCCCTT GGTAACCACC 
AAAGCATTGC AAATGGTGCA GCTGAGGTCT ATCAGAAAAG CAACAGAAGG TGACCCATCA 
CCTGAATCTG CTTCTGAAAC CATCTCTCAG GAAAAGGGTA TTGTCACTTC ATCATCACAG 
TCTTCCCTAA GGCCATCTCT CTCACTACGA CTCAGTAACA GCTTAGGTGA AGAGGAGATG 
AAGATTCATG GCACTTCATT TAAAAACACA CCTCAAACAC TGGCTCAGAG TTCTCCTCTG 
GTACTCTCTG ACAACGTACC CATGCTTGAG AGCGATCAAA AAGCTGCAGT TACTATGGGT 
CTAAGCAAGT CTTTGGAACC AGATTCCCCA GGGTCAGCTC CAGAAGCCAC ACCCAAGTCC 
CCAGTTCGGA GCGAAGACTT CCATTCTGGC ATGCCACTGC AGGGGTCACC AGCATCTCCA 
GACAAGACTC AGGTCGTTTT GCCAAACAAG AAACCACCTC CCATTTCAAA GAAACCCAAA 
CTGTTCCTTG TTGTACCACC TCCACAGTTA GATCTCACAG TGGAGAAAAT AGCTGAAGTG 
AGTGATACTG TCAGAAGCAC ATCAAGCCCA ACTAAGAGAG ACGCTGTGCT TACACACTGC 
GAGGAGGCGA GAAATTGCCT TACAGATGGA CTCAGCTCCA GCGAGATGGA CTCTGGCAGC 
CTGGTTCCTG AGGGAGGAGC TGCCAGAGTC ACCTTCTCTG AAGCAGTGGG AGTTGGTGCC 
TTTGTGGTAC AGTCCGCTGC ATCACCAGTT CAAGAGGAGC CCAGGCAAGA GGAGCAGTCA 
GTTTTTGATG AAGGAAGCAG CTCAGGCAGC AGCCAAGACA GCAGCAGTAA CACTGAAGGA 
CACCCGTCTC AGGAGAACGA AAGTGCCGAG GTGTTTGAAT CAGAAACAGC AAATAGTTCA 
TTCCTGCCAA GCAATAATTA TGGGGAAGAG ACGGATGGAG TGGCAACACC AGCGAGACCG 
AGGACTACTG AGGATCTTTT TGCAGCCATT CACAGATCCA AAAGGAAAGT CCTTGGCCGT 
AAGGATTCTG AGGACGATCG TACCCGCAAC CATTCTCCTT CACCCCCCGT AACACCCACT 
GGTGCTTCCC CAACCTTATC TACCCTCAAA CAGGCAGGAT CTATTCAGAG AAGCGTTCGC 
AAGAGCAGCA CCAGCAATGA CAACTTCAAA GCCCTGCTGC TGAAGAAAGG GAGTCGCTGT 
GACACCAGTT CCCGCATGTC AGCAGCAGAG ATGTTGAAGC ACACGGATCC ACGGTTCCAC 
CGAGCCAAGG TGGATGCCTC TCCCGACCTT TCCGACAGCC CTGCCAGCTG CTCGCCCAGC 
AAGAGCAAAC GGGCCCAAGA GGAGTGGGCC AAGAGTGAAG GCCTGATGCC GAGGAGCATG 
TCCCTCTCCA GCACTAGGTA TGGCCGATCA CGAACACCGC CCTCCGCTGC CAGCAGCAAG 
TACAATGTTC GCAACCGCAT CCAGAGCAGC CCCATGACAG TGATTAGCGA AGGAGATGGG 
GAAGTGGTGG AGCAGACAGA GAGCAGGGTC CAAAGGACTT TGGATGAGCA GCGAGAGAGG 
CAGCTCGATG TATTTAACAG TGATGAAATA GACATGAATG ACTTTTCGTA TTCTGAGGAG 
CTGGACTGCA AGGAGACCTT GGATCCAACC CATCTGGACT TAATGACTCA GCCGGGTACT 
TCGAGAAAGT ACCTAAGTCC TTCAGCTGAA GAAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004935747.1
CDS	1327..6285
Translation	MKKECVSRSFKLKQNAGSLSRAVSWINFSSLSRQTKRLFRSDGELSSICVQQVDEDDENWTYRSPRGKAVSNLDEESRWTVHYTAPWHQQENVFLPSSRPPCVEDLHRQAKLNLKSVLRECDKLRRDGYRSSQYYSQGPTFSSSSSAICGSYQDDYEEIEQKCSVSSPEEEKLITIKRPKTPVSNDLSDINTQTNWTKSLPLPTPEEKMRQQAQAVQTDVVPINVTAVGTGQSDFRGHSMYVPDHSSTLGRLDSYRSAMQRSETKDTSCQTEEVKVVPPSMRRIRAQKGQGIAAQMSQFSSSSGNMSVMSDSAAVIFASRQNSDIGFHSLPRAGARVSLQSLEQTQSISRQTEDIAGTLPHQMSKLQVDDDVAHLRNNAITGTVSRPKSQEVRSCDSEKSTSPACVVSPHATYSTSVIPNATLSSSSEVIAIHTAQSTGSLDNKISSSAVYPKPRDNPVANNAISGKEDHHSSSGNWSESSSTRHSQTSDTIPSHTVMMLSLGDSAVSLSTPGNVEAGSQSVNYSCRSNLASPNQSQDSDGRSESSYSGERTQGVVSSTEHWLYKSAENSETASCKVACTTPGCATPGSNLSSSSLERTSAKEDSTSLYSVDHDGYYSSMSMDSGLKSDMPCYSTNGFGNPRDSVINVFEAKEKKHQDDQPGQGEKSLARNISLKKAKKPPLPPSRTDSLRRVPKKKAQSNGQVLDETLIATLQHSLQLNLKCKNGSSPSQSPCSDYEDPWVLRSRSQSSVSASSSMMSTTAPNLYSICTVTPSQSETSSIKSEYADQWGYYSDYAAVADDQMKSPVTHSASTSSALSDYSISHFSDGSRASVPQVPAGLAKPKSTSPEKSHRVTSPSSGYSSQSNTPTALTPVPVFLKSASSGNGKSKLKPKVPERKSSLLSSVSISSSSTSLSSNTSTEGSVNVKKLDPTPSSPPDSGAPLPPPPLLAPPPHCLELSPPSSPPPPPAEAVDISPLPASPTFPPPPPEADVNSSFAHSVQWLPQEASIGSYSSPVPPPVFPLVVPPPAPPLDPKLTKGAMICPQYSFKKHDQEDSCYNAVKQPFSKQDASRPVMPLVTTKALQMVQLRSIRKATEGDPSPESASETISQEKGIVTSSSQSSLRPSLSLRLSNSLGEEEMKIHGTSFKNTPQTLAQSSPLVLSDNVPMLESDQKAAVTMGLSKSLEPDSPGSAPEATPKSPVRSEDFHSGMPLQGSPASPDKTQVVLPNKKPPPISKKPKLFLVVPPPQLDLTVEKIAEVSDTVRSTSSPTKRDAVLTHCEEARNCLTDGLSSSEMDSGSLVPEGGAARVTFSEAVGVGAFVVQSAASPVQEEPRQEEQSVFDEGSSSGSSQDSSSNTEGHPSQENESAEVFESETANSSFLPSNNYGEETDGVATPARPRTTEDLFAAIHRSKRKVLGRKDSEDDRTRNHSPSPPVTPTGASPTLSTLKQAGSIQRSVRKSSTSNDNFKALLLKKGSRCDTSSRMSAAEMLKHTDPRFHRAKVDASPDLSDSPASCSPSKSKRAQEEWAKSEGLMPRSMSLSSTRYGRSRTPPSAASSKYNVRNRIQSSPMTVISEGDGEVVEQTESRVQRTLDEQRERQLDVFNSDEIDMNDFSYSEELDCKETLDPTHLDLMTQPGTSRKYLSPSAEES

Target ORF information:

RefSeq Version	XM_004935690.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NHS like 1 (NHSL1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004935690.3

ORF Insert Sequence:

ATGAAAAAGG AGTGTGTTTC GAGATCTTTC AAACTCAAAC AAAACGCAGG CTCCCTCTCG 
CGTGCTGTAA GCTGGATAAA TTTCTCCTCC TTGTCAAGGC AGACAAAGAG ATTGTTTCGC 
AGCGATGGTG AGCTCTCCTC CATTTGCGTG CAGCAGGTAG ACGAGGATGA TGAGAACTGG 
ACCTACAGGA GCCCACGCGG AAAAGCTGTT TCCAACTTAG ATGAAGAGAG CAGATGGACC 
GTCCATTACA CTGCTCCATG GCACCAGCAG GAAAATGTGT TCCTGCCTTC CTCAAGGCCG 
CCTTGTGTGG AGGACTTGCA CCGGCAAGCA AAGCTGAACC TCAAGTCAGT ACTGAGAGAG 
TGTGACAAGC TGCGGAGAGA TGGCTATCGG AGCTCTCAGT ATTACTCTCA GGGCCCCACC 
TTCTCCTCAT CCTCCAGTGC AATCTGTGGA AGTTACCAGG ACGATTATGA AGAAATTGAA 
CAAAAGTGTT CTGTCTCTTC CCCAGAAGAA GAAAAGCTTA TCACCATCAA AAGGCCTAAA 
ACCCCAGTTT CAAATGACTT ATCAGATATC AACACACAAA CCAACTGGAC TAAGTCACTT 
CCACTGCCAA CACCAGAAGA GAAGATGCGA CAGCAAGCAC AGGCAGTCCA AACAGATGTG 
GTTCCTATCA ATGTGACTGC AGTTGGCACA GGCCAAAGTG ATTTCCGAGG GCACTCGATG 
TATGTCCCAG ATCACAGTTC CACGCTAGGG CGACTAGACA GCTATCGCTC TGCTATGCAG 
CGGTCTGAAA CCAAGGACAC CAGCTGCCAG ACTGAAGAAG TTAAAGTTGT GCCTCCTTCG 
ATGAGAAGAA TACGAGCACA GAAAGGACAA GGCATTGCAG CTCAGATGTC TCAGTTCTCC 
AGCTCATCTG GAAACATGTC TGTGATGAGT GATTCTGCTG CAGTTATATT TGCTTCTCGC 
CAGAATAGTG ACATAGGTTT TCACAGCTTG CCTCGGGCTG GTGCAAGAGT GTCTCTGCAG 
TCCTTAGAAC AGACCCAGAG CATCTCCAGA CAGACAGAAG ACATTGCTGG TACTTTGCCT 
CACCAGATGA GTAAACTGCA AGTGGATGAT GATGTTGCAC ATCTGAGAAA TAATGCCATA 
ACAGGAACTG TTTCGAGGCC AAAGTCTCAA GAGGTAAGAA GTTGTGACAG TGAAAAGTCC 
ACTAGCCCAG CATGTGTGGT CTCTCCTCAT GCCACCTACT CAACAAGCGT CATACCCAAT 
GCGACACTGT CATCCTCCTC AGAAGTTATT GCTATTCACA CTGCCCAGAG TACTGGATCG 
CTGGATAACA AAATTAGCAG TTCTGCTGTG TACCCAAAAC CAAGAGATAA TCCTGTTGCC 
AACAATGCAA TAAGTGGGAA AGAAGATCAC CATTCTTCAA GTGGTAACTG GAGTGAGAGT 
AGCTCCACAC GTCACTCACA GACTTCAGAT ACCATCCCAT CTCATACTGT CATGATGCTT 
TCTCTTGGTG ACTCTGCTGT CTCTCTCAGC ACTCCTGGAA ATGTAGAGGC TGGCTCTCAG 
AGTGTGAACT ACAGCTGCAG AAGCAATCTT GCTTCACCAA ACCAGTCCCA GGACAGCGAT 
GGTAGGAGTG AATCTAGCTA CTCAGGGGAG CGAACACAAG GTGTAGTGAG CAGCACAGAG 
CACTGGCTAT ACAAGTCTGC GGAAAATAGT GAGACTGCAT CATGCAAGGT AGCTTGTACC 
ACACCAGGCT GTGCCACTCC TGGTAGCAAC CTGAGCAGCA GCAGCCTGGA GAGGACATCA 
GCCAAAGAAG ATTCCACTTC ACTGTATTCT GTAGACCACG ATGGTTATTA CAGCTCTATG 
AGCATGGACT CTGGACTGAA GTCAGACATG CCATGCTATA GTACTAATGG TTTTGGGAAC 
CCCAGAGACA GCGTGATTAA TGTCTTTGAA GCAAAGGAGA AGAAACATCA AGATGACCAG 
CCAGGCCAAG GTGAAAAATC CCTTGCAAGA AACATCTCTC TAAAAAAGGC AAAGAAGCCG 
CCTTTACCTC CGTCTAGAAC AGACTCACTC AGAAGGGTAC CCAAGAAGAA AGCTCAGTCC 
AATGGACAGG TACTTGATGA AACCCTCATT GCTACCCTCC AGCATTCTCT GCAGTTGAAT 
CTCAAGTGCA AAAATGGCAG CTCCCCTTCC CAGAGCCCCT GCAGTGACTA TGAGGATCCC 
TGGGTGTTGC GCTCTCGCAG CCAAAGCTCG GTCAGCGCAA GCAGCAGCAT GATGTCCACT 
ACTGCCCCAA ACCTGTACTC CATTTGCACA GTCACTCCCT CTCAGAGTGA AACAAGCAGC 
ATCAAGTCAG AGTATGCTGA CCAGTGGGGT TACTACAGTG ACTATGCTGC AGTGGCAGAT 
GACCAGATGA AGTCTCCAGT GACCCATTCT GCTAGTACCT CTTCTGCTCT CAGTGATTAC 
AGCATCAGCC ACTTCAGTGA TGGCTCAAGG GCTTCTGTAC CACAAGTGCC CGCTGGACTG 
GCCAAACCAA AGAGCACTTC TCCAGAGAAG TCCCACCGAG TCACATCGCC ATCCAGTGGG 
TACTCCAGCC AGTCCAATAC ACCCACTGCA CTCACTCCAG TGCCTGTGTT TTTAAAATCT 
GCGTCATCAG GAAATGGGAA ATCCAAGCTG AAACCTAAAG TGCCTGAAAG GAAATCTTCT 
CTTCTGTCTT CAGTCTCCAT TTCTTCATCC TCTACTTCTC TTTCTTCAAA TACATCTACT 
GAAGGGAGTG TGAATGTGAA GAAATTGGAC CCCACCCCGA GTTCTCCTCC AGATTCTGGT 
GCACCTCTTC CTCCGCCTCC TCTTCTAGCA CCTCCTCCAC ATTGCCTTGA GCTCTCTCCT 
CCCTCTTCCC CTCCTCCTCC TCCAGCAGAA GCCGTGGATA TATCACCATT GCCAGCTTCT 
CCCACATTCC CCCCCCCTCC ACCAGAAGCT GATGTAAATT CTTCCTTTGC TCACAGTGTC 
CAGTGGTTAC CACAAGAAGC TTCCATCGGT TCGTACTCTT CCCCTGTTCC TCCTCCTGTT 
TTCCCTTTAG TTGTCCCACC ACCAGCCCCA CCTCTTGATC CCAAACTGAC AAAAGGTGCA 
ATGATATGCC CACAGTATTC ATTTAAGAAA CATGATCAGG AAGATTCTTG CTACAATGCT 
GTGAAACAGC CATTCAGCAA GCAAGATGCA TCAAGACCTG TGATGCCCTT GGTAACCACC 
AAAGCATTGC AAATGGTGCA GCTGAGGTCT ATCAGAAAAG CAACAGAAGG TGACCCATCA 
CCTGAATCTG CTTCTGAAAC CATCTCTCAG GAAAAGGGTA TTGTCACTTC ATCATCACAG 
TCTTCCCTAA GGCCATCTCT CTCACTACGA CTCAGTAACA GCTTAGGTGA AGAGGAGATG 
AAGATTCATG GCACTTCATT TAAAAACACA CCTCAAACAC TGGCTCAGAG TTCTCCTCTG 
GTACTCTCTG ACAACGTACC CATGCTTGAG AGCGATCAAA AAGCTGCAGT TACTATGGGT 
CTAAGCAAGT CTTTGGAACC AGATTCCCCA GGGTCAGCTC CAGAAGCCAC ACCCAAGTCC 
CCAGTTCGGA GCGAAGACTT CCATTCTGGC ATGCCACTGC AGGGGTCACC AGCATCTCCA 
GACAAGACTC AGGTCGTTTT GCCAAACAAG AAACCACCTC CCATTTCAAA GAAACCCAAA 
CTGTTCCTTG TTGTACCACC TCCACAGTTA GATCTCACAG TGGAGAAAAT AGCTGAAGTG 
AGTGATACTG TCAGAAGCAC ATCAAGCCCA ACTAAGAGAG ACGCTGTGCT TACACACTGC 
GAGGAGGCGA GAAATTGCCT TACAGATGGA CTCAGCTCCA GCGAGATGGA CTCTGGCAGC 
CTGGTTCCTG AGGGAGGAGC TGCCAGAGTC ACCTTCTCTG AAGCAGTGGG AGTTGGTGCC 
TTTGTGGTAC AGTCCGCTGC ATCACCAGTT CAAGAGGAGC CCAGGCAAGA GGAGCAGTCA 
GTTTTTGATG AAGGAAGCAG CTCAGGCAGC AGCCAAGACA GCAGCAGTAA CACTGAAGGA 
CACCCGTCTC AGGAGAACGA AAGTGCCGAG GTGTTTGAAT CAGAAACAGC AAATAGTTCA 
TTCCTGCCAA GCAATAATTA TGGGGAAGAG ACGGATGGAG TGGCAACACC AGCGAGACCG 
AGGACTACTG AGGATCTTTT TGCAGCCATT CACAGATCCA AAAGGAAAGT CCTTGGCCGT 
AAGGATTCTG AGGACGATCG TACCCGCAAC CATTCTCCTT CACCCCCCGT AACACCCACT 
GGTGCTTCCC CAACCTTATC TACCCTCAAA CAGGCAGGAT CTATTCAGAG AAGCGTTCGC 
AAGAGCAGCA CCAGCAATGA CAACTTCAAA GCCCTGCTGC TGAAGAAAGG GAGTCGCTGT 
GACACCAGTT CCCGCATGTC AGCAGCAGAG ATGTTGAAGC ACACGGATCC ACGGTTCCAC 
CGAGCCAAGG TGGATGCCTC TCCCGACCTT TCCGACAGCC CTGCCAGCTG CTCGCCCAGC 
AAGAGCAAAC GGGCCCAAGA GGAGTGGGCC AAGAGTGAAG GCCTGATGCC GAGGAGCATG 
TCCCTCTCCA GCACTAGGTA TGGCCGATCA CGAACACCGC CCTCCGCTGC CAGCAGCAAG 
TACAATGTTC GCAACCGCAT CCAGAGCAGC CCCATGACAG TGATTAGCGA AGGAGATGGG 
GAAGTGGTGG AGCAGACAGA GAGCAGGGTC CAAAGGACTT TGGATGAGCA GCGAGAGAGG 
CAGCTCGATG TATTTAACAG TGATGAAATA GACATGAATG ACTTTTCGTA TTCTGAGGAG 
CTGGACTGCA AGGAGACCTT GGATCCAACC CATCTGGACT TAATGACTCA GCCGGGTACT 
TCGAGAAAGT ACCTAAGTCC TTCAGCTGAA GAAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_004935690.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004935747.1 Gallus gallus NHS-like 1 (NHSL1), transcript variant X7, mRNA.

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CloneID	OGa06407
Clone ID Related Accession (Same CDS sequence)	XM_004935690.3 , XM_004935690.2
Accession Version	XM_004935690.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	NHS-like protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455837.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004935690.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAGG AGTGTGTTTC GAGATCTTTC AAACTCAAAC AAAACGCAGG CTCCCTCTCG 
CGTGCTGTAA GCTGGATAAA TTTCTCCTCC TTGTCAAGGC AGACAAAGAG ATTGTTTCGC 
AGCGATGGTG AGCTCTCCTC CATTTGCGTG CAGCAGGTAG ACGAGGATGA TGAGAACTGG 
ACCTACAGGA GCCCACGCGG AAAAGCTGTT TCCAACTTAG ATGAAGAGAG CAGATGGACC 
GTCCATTACA CTGCTCCATG GCACCAGCAG GAAAATGTGT TCCTGCCTTC CTCAAGGCCG 
CCTTGTGTGG AGGACTTGCA CCGGCAAGCA AAGCTGAACC TCAAGTCAGT ACTGAGAGAG 
TGTGACAAGC TGCGGAGAGA TGGCTATCGG AGCTCTCAGT ATTACTCTCA GGGCCCCACC 
TTCTCCTCAT CCTCCAGTGC AATCTGTGGA AGTTACCAGG ACGATTATGA AGAAATTGAA 
CAAAAGTGTT CTGTCTCTTC CCCAGAAGAA GAAAAGCTTA TCACCATCAA AAGGCCTAAA 
ACCCCAGTTT CAAATGACTT ATCAGATATC AACACACAAA CCAACTGGAC TAAGTCACTT 
CCACTGCCAA CACCAGAAGA GAAGATGCGA CAGCAAGCAC AGGCAGTCCA AACAGATGTG 
GTTCCTATCA ATGTGACTGC AGTTGGCACA GGCCAAAGTG ATTTCCGAGG GCACTCGATG 
TATGTCCCAG ATCACAGTTC CACGCTAGGG CGACTAGACA GCTATCGCTC TGCTATGCAG 
CGGTCTGAAA CCAAGGACAC CAGCTGCCAG ACTGAAGAAG TTAAAGTTGT GCCTCCTTCG 
ATGAGAAGAA TACGAGCACA GAAAGGACAA GGCATTGCAG CTCAGATGTC TCAGTTCTCC 
AGCTCATCTG GAAACATGTC TGTGATGAGT GATTCTGCTG CAGTTATATT TGCTTCTCGC 
CAGAATAGTG ACATAGGTTT TCACAGCTTG CCTCGGGCTG GTGCAAGAGT GTCTCTGCAG 
TCCTTAGAAC AGACCCAGAG CATCTCCAGA CAGACAGAAG ACATTGCTGG TACTTTGCCT 
CACCAGATGA GTAAACTGCA AGTGGATGAT GATGTTGCAC ATCTGAGAAA TAATGCCATA 
ACAGGAACTG TTTCGAGGCC AAAGTCTCAA GAGGTAAGAA GTTGTGACAG TGAAAAGTCC 
ACTAGCCCAG CATGTGTGGT CTCTCCTCAT GCCACCTACT CAACAAGCGT CATACCCAAT 
GCGACACTGT CATCCTCCTC AGAAGTTATT GCTATTCACA CTGCCCAGAG TACTGGATCG 
CTGGATAACA AAATTAGCAG TTCTGCTGTG TACCCAAAAC CAAGAGATAA TCCTGTTGCC 
AACAATGCAA TAAGTGGGAA AGAAGATCAC CATTCTTCAA GTGGTAACTG GAGTGAGAGT 
AGCTCCACAC GTCACTCACA GACTTCAGAT ACCATCCCAT CTCATACTGT CATGATGCTT 
TCTCTTGGTG ACTCTGCTGT CTCTCTCAGC ACTCCTGGAA ATGTAGAGGC TGGCTCTCAG 
AGTGTGAACT ACAGCTGCAG AAGCAATCTT GCTTCACCAA ACCAGTCCCA GGACAGCGAT 
GGTAGGAGTG AATCTAGCTA CTCAGGGGAG CGAACACAAG GTGTAGTGAG CAGCACAGAG 
CACTGGCTAT ACAAGTCTGC GGAAAATAGT GAGACTGCAT CATGCAAGGT AGCTTGTACC 
ACACCAGGCT GTGCCACTCC TGGTAGCAAC CTGAGCAGCA GCAGCCTGGA GAGGACATCA 
GCCAAAGAAG ATTCCACTTC ACTGTATTCT GTAGACCACG ATGGTTATTA CAGCTCTATG 
AGCATGGACT CTGGACTGAA GTCAGACATG CCATGCTATA GTACTAATGG TTTTGGGAAC 
CCCAGAGACA GCGTGATTAA TGTCTTTGAA GCAAAGGAGA AGAAACATCA AGATGACCAG 
CCAGGCCAAG GTGAAAAATC CCTTGCAAGA AACATCTCTC TAAAAAAGGC AAAGAAGCCG 
CCTTTACCTC CGTCTAGAAC AGACTCACTC AGAAGGGTAC CCAAGAAGAA AGCTCAGTCC 
AATGGACAGG TACTTGATGA AACCCTCATT GCTACCCTCC AGCATTCTCT GCAGTTGAAT 
CTCAAGTGCA AAAATGGCAG CTCCCCTTCC CAGAGCCCCT GCAGTGACTA TGAGGATCCC 
TGGGTGTTGC GCTCTCGCAG CCAAAGCTCG GTCAGCGCAA GCAGCAGCAT GATGTCCACT 
ACTGCCCCAA ACCTGTACTC CATTTGCACA GTCACTCCCT CTCAGAGTGA AACAAGCAGC 
ATCAAGTCAG AGTATGCTGA CCAGTGGGGT TACTACAGTG ACTATGCTGC AGTGGCAGAT 
GACCAGATGA AGTCTCCAGT GACCCATTCT GCTAGTACCT CTTCTGCTCT CAGTGATTAC 
AGCATCAGCC ACTTCAGTGA TGGCTCAAGG GCTTCTGTAC CACAAGTGCC CGCTGGACTG 
GCCAAACCAA AGAGCACTTC TCCAGAGAAG TCCCACCGAG TCACATCGCC ATCCAGTGGG 
TACTCCAGCC AGTCCAATAC ACCCACTGCA CTCACTCCAG TGCCTGTGTT TTTAAAATCT 
GCGTCATCAG GAAATGGGAA ATCCAAGCTG AAACCTAAAG TGCCTGAAAG GAAATCTTCT 
CTTCTGTCTT CAGTCTCCAT TTCTTCATCC TCTACTTCTC TTTCTTCAAA TACATCTACT 
GAAGGGAGTG TGAATGTGAA GAAATTGGAC CCCACCCCGA GTTCTCCTCC AGATTCTGGT 
GCACCTCTTC CTCCGCCTCC TCTTCTAGCA CCTCCTCCAC ATTGCCTTGA GCTCTCTCCT 
CCCTCTTCCC CTCCTCCTCC TCCAGCAGAA GCCGTGGATA TATCACCATT GCCAGCTTCT 
CCCACATTCC CCCCCCCTCC ACCAGAAGCT GATGTAAATT CTTCCTTTGC TCACAGTGTC 
CAGTGGTTAC CACAAGAAGC TTCCATCGGT TCGTACTCTT CCCCTGTTCC TCCTCCTGTT 
TTCCCTTTAG TTGTCCCACC ACCAGCCCCA CCTCTTGATC CCAAACTGAC AAAAGGTGCA 
ATGATATGCC CACAGTATTC ATTTAAGAAA CATGATCAGG AAGATTCTTG CTACAATGCT 
GTGAAACAGC CATTCAGCAA GCAAGATGCA TCAAGACCTG TGATGCCCTT GGTAACCACC 
AAAGCATTGC AAATGGTGCA GCTGAGGTCT ATCAGAAAAG CAACAGAAGG TGACCCATCA 
CCTGAATCTG CTTCTGAAAC CATCTCTCAG GAAAAGGGTA TTGTCACTTC ATCATCACAG 
TCTTCCCTAA GGCCATCTCT CTCACTACGA CTCAGTAACA GCTTAGGTGA AGAGGAGATG 
AAGATTCATG GCACTTCATT TAAAAACACA CCTCAAACAC TGGCTCAGAG TTCTCCTCTG 
GTACTCTCTG ACAACGTACC CATGCTTGAG AGCGATCAAA AAGCTGCAGT TACTATGGGT 
CTAAGCAAGT CTTTGGAACC AGATTCCCCA GGGTCAGCTC CAGAAGCCAC ACCCAAGTCC 
CCAGTTCGGA GCGAAGACTT CCATTCTGGC ATGCCACTGC AGGGGTCACC AGCATCTCCA 
GACAAGACTC AGGTCGTTTT GCCAAACAAG AAACCACCTC CCATTTCAAA GAAACCCAAA 
CTGTTCCTTG TTGTACCACC TCCACAGTTA GATCTCACAG TGGAGAAAAT AGCTGAAGTG 
AGTGATACTG TCAGAAGCAC ATCAAGCCCA ACTAAGAGAG ACGCTGTGCT TACACACTGC 
GAGGAGGCGA GAAATTGCCT TACAGATGGA CTCAGCTCCA GCGAGATGGA CTCTGGCAGC 
CTGGTTCCTG AGGGAGGAGC TGCCAGAGTC ACCTTCTCTG AAGCAGTGGG AGTTGGTGCC 
TTTGTGGTAC AGTCCGCTGC ATCACCAGTT CAAGAGGAGC CCAGGCAAGA GGAGCAGTCA 
GTTTTTGATG AAGGAAGCAG CTCAGGCAGC AGCCAAGACA GCAGCAGTAA CACTGAAGGA 
CACCCGTCTC AGGAGAACGA AAGTGCCGAG GTGTTTGAAT CAGAAACAGC AAATAGTTCA 
TTCCTGCCAA GCAATAATTA TGGGGAAGAG ACGGATGGAG TGGCAACACC AGCGAGACCG 
AGGACTACTG AGGATCTTTT TGCAGCCATT CACAGATCCA AAAGGAAAGT CCTTGGCCGT 
AAGGATTCTG AGGACGATCG TACCCGCAAC CATTCTCCTT CACCCCCCGT AACACCCACT 
GGTGCTTCCC CAACCTTATC TACCCTCAAA CAGGCAGGAT CTATTCAGAG AAGCGTTCGC 
AAGAGCAGCA CCAGCAATGA CAACTTCAAA GCCCTGCTGC TGAAGAAAGG GAGTCGCTGT 
GACACCAGTT CCCGCATGTC AGCAGCAGAG ATGTTGAAGC ACACGGATCC ACGGTTCCAC 
CGAGCCAAGG TGGATGCCTC TCCCGACCTT TCCGACAGCC CTGCCAGCTG CTCGCCCAGC 
AAGAGCAAAC GGGCCCAAGA GGAGTGGGCC AAGAGTGAAG GCCTGATGCC GAGGAGCATG 
TCCCTCTCCA GCACTAGGTA TGGCCGATCA CGAACACCGC CCTCCGCTGC CAGCAGCAAG 
TACAATGTTC GCAACCGCAT CCAGAGCAGC CCCATGACAG TGATTAGCGA AGGAGATGGG 
GAAGTGGTGG AGCAGACAGA GAGCAGGGTC CAAAGGACTT TGGATGAGCA GCGAGAGAGG 
CAGCTCGATG TATTTAACAG TGATGAAATA GACATGAATG ACTTTTCGTA TTCTGAGGAG 
CTGGACTGCA AGGAGACCTT GGATCCAACC CATCTGGACT TAATGACTCA GCCGGGTACT 
TCGAGAAAGT ACCTAAGTCC TTCAGCTGAA GAAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004935747.1
CDS	617..5575
Translation	MKKECVSRSFKLKQNAGSLSRAVSWINFSSLSRQTKRLFRSDGELSSICVQQVDEDDENWTYRSPRGKAVSNLDEESRWTVHYTAPWHQQENVFLPSSRPPCVEDLHRQAKLNLKSVLRECDKLRRDGYRSSQYYSQGPTFSSSSSAICGSYQDDYEEIEQKCSVSSPEEEKLITIKRPKTPVSNDLSDINTQTNWTKSLPLPTPEEKMRQQAQAVQTDVVPINVTAVGTGQSDFRGHSMYVPDHSSTLGRLDSYRSAMQRSETKDTSCQTEEVKVVPPSMRRIRAQKGQGIAAQMSQFSSSSGNMSVMSDSAAVIFASRQNSDIGFHSLPRAGARVSLQSLEQTQSISRQTEDIAGTLPHQMSKLQVDDDVAHLRNNAITGTVSRPKSQEVRSCDSEKSTSPACVVSPHATYSTSVIPNATLSSSSEVIAIHTAQSTGSLDNKISSSAVYPKPRDNPVANNAISGKEDHHSSSGNWSESSSTRHSQTSDTIPSHTVMMLSLGDSAVSLSTPGNVEAGSQSVNYSCRSNLASPNQSQDSDGRSESSYSGERTQGVVSSTEHWLYKSAENSETASCKVACTTPGCATPGSNLSSSSLERTSAKEDSTSLYSVDHDGYYSSMSMDSGLKSDMPCYSTNGFGNPRDSVINVFEAKEKKHQDDQPGQGEKSLARNISLKKAKKPPLPPSRTDSLRRVPKKKAQSNGQVLDETLIATLQHSLQLNLKCKNGSSPSQSPCSDYEDPWVLRSRSQSSVSASSSMMSTTAPNLYSICTVTPSQSETSSIKSEYADQWGYYSDYAAVADDQMKSPVTHSASTSSALSDYSISHFSDGSRASVPQVPAGLAKPKSTSPEKSHRVTSPSSGYSSQSNTPTALTPVPVFLKSASSGNGKSKLKPKVPERKSSLLSSVSISSSSTSLSSNTSTEGSVNVKKLDPTPSSPPDSGAPLPPPPLLAPPPHCLELSPPSSPPPPPAEAVDISPLPASPTFPPPPPEADVNSSFAHSVQWLPQEASIGSYSSPVPPPVFPLVVPPPAPPLDPKLTKGAMICPQYSFKKHDQEDSCYNAVKQPFSKQDASRPVMPLVTTKALQMVQLRSIRKATEGDPSPESASETISQEKGIVTSSSQSSLRPSLSLRLSNSLGEEEMKIHGTSFKNTPQTLAQSSPLVLSDNVPMLESDQKAAVTMGLSKSLEPDSPGSAPEATPKSPVRSEDFHSGMPLQGSPASPDKTQVVLPNKKPPPISKKPKLFLVVPPPQLDLTVEKIAEVSDTVRSTSSPTKRDAVLTHCEEARNCLTDGLSSSEMDSGSLVPEGGAARVTFSEAVGVGAFVVQSAASPVQEEPRQEEQSVFDEGSSSGSSQDSSSNTEGHPSQENESAEVFESETANSSFLPSNNYGEETDGVATPARPRTTEDLFAAIHRSKRKVLGRKDSEDDRTRNHSPSPPVTPTGASPTLSTLKQAGSIQRSVRKSSTSNDNFKALLLKKGSRCDTSSRMSAAEMLKHTDPRFHRAKVDASPDLSDSPASCSPSKSKRAQEEWAKSEGLMPRSMSLSSTRYGRSRTPPSAASSKYNVRNRIQSSPMTVISEGDGEVVEQTESRVQRTLDEQRERQLDVFNSDEIDMNDFSYSEELDCKETLDPTHLDLMTQPGTSRKYLSPSAEES

Target ORF information:

RefSeq Version	XM_004935690.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NHS-like 1 (NHSL1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004935690.2

ORF Insert Sequence:

ATGAAAAAGG AGTGTGTTTC GAGATCTTTC AAACTCAAAC AAAACGCAGG CTCCCTCTCG 
CGTGCTGTAA GCTGGATAAA TTTCTCCTCC TTGTCAAGGC AGACAAAGAG ATTGTTTCGC 
AGCGATGGTG AGCTCTCCTC CATTTGCGTG CAGCAGGTAG ACGAGGATGA TGAGAACTGG 
ACCTACAGGA GCCCACGCGG AAAAGCTGTT TCCAACTTAG ATGAAGAGAG CAGATGGACC 
GTCCATTACA CTGCTCCATG GCACCAGCAG GAAAATGTGT TCCTGCCTTC CTCAAGGCCG 
CCTTGTGTGG AGGACTTGCA CCGGCAAGCA AAGCTGAACC TCAAGTCAGT ACTGAGAGAG 
TGTGACAAGC TGCGGAGAGA TGGCTATCGG AGCTCTCAGT ATTACTCTCA GGGCCCCACC 
TTCTCCTCAT CCTCCAGTGC AATCTGTGGA AGTTACCAGG ACGATTATGA AGAAATTGAA 
CAAAAGTGTT CTGTCTCTTC CCCAGAAGAA GAAAAGCTTA TCACCATCAA AAGGCCTAAA 
ACCCCAGTTT CAAATGACTT ATCAGATATC AACACACAAA CCAACTGGAC TAAGTCACTT 
CCACTGCCAA CACCAGAAGA GAAGATGCGA CAGCAAGCAC AGGCAGTCCA AACAGATGTG 
GTTCCTATCA ATGTGACTGC AGTTGGCACA GGCCAAAGTG ATTTCCGAGG GCACTCGATG 
TATGTCCCAG ATCACAGTTC CACGCTAGGG CGACTAGACA GCTATCGCTC TGCTATGCAG 
CGGTCTGAAA CCAAGGACAC CAGCTGCCAG ACTGAAGAAG TTAAAGTTGT GCCTCCTTCG 
ATGAGAAGAA TACGAGCACA GAAAGGACAA GGCATTGCAG CTCAGATGTC TCAGTTCTCC 
AGCTCATCTG GAAACATGTC TGTGATGAGT GATTCTGCTG CAGTTATATT TGCTTCTCGC 
CAGAATAGTG ACATAGGTTT TCACAGCTTG CCTCGGGCTG GTGCAAGAGT GTCTCTGCAG 
TCCTTAGAAC AGACCCAGAG CATCTCCAGA CAGACAGAAG ACATTGCTGG TACTTTGCCT 
CACCAGATGA GTAAACTGCA AGTGGATGAT GATGTTGCAC ATCTGAGAAA TAATGCCATA 
ACAGGAACTG TTTCGAGGCC AAAGTCTCAA GAGGTAAGAA GTTGTGACAG TGAAAAGTCC 
ACTAGCCCAG CATGTGTGGT CTCTCCTCAT GCCACCTACT CAACAAGCGT CATACCCAAT 
GCGACACTGT CATCCTCCTC AGAAGTTATT GCTATTCACA CTGCCCAGAG TACTGGATCG 
CTGGATAACA AAATTAGCAG TTCTGCTGTG TACCCAAAAC CAAGAGATAA TCCTGTTGCC 
AACAATGCAA TAAGTGGGAA AGAAGATCAC CATTCTTCAA GTGGTAACTG GAGTGAGAGT 
AGCTCCACAC GTCACTCACA GACTTCAGAT ACCATCCCAT CTCATACTGT CATGATGCTT 
TCTCTTGGTG ACTCTGCTGT CTCTCTCAGC ACTCCTGGAA ATGTAGAGGC TGGCTCTCAG 
AGTGTGAACT ACAGCTGCAG AAGCAATCTT GCTTCACCAA ACCAGTCCCA GGACAGCGAT 
GGTAGGAGTG AATCTAGCTA CTCAGGGGAG CGAACACAAG GTGTAGTGAG CAGCACAGAG 
CACTGGCTAT ACAAGTCTGC GGAAAATAGT GAGACTGCAT CATGCAAGGT AGCTTGTACC 
ACACCAGGCT GTGCCACTCC TGGTAGCAAC CTGAGCAGCA GCAGCCTGGA GAGGACATCA 
GCCAAAGAAG ATTCCACTTC ACTGTATTCT GTAGACCACG ATGGTTATTA CAGCTCTATG 
AGCATGGACT CTGGACTGAA GTCAGACATG CCATGCTATA GTACTAATGG TTTTGGGAAC 
CCCAGAGACA GCGTGATTAA TGTCTTTGAA GCAAAGGAGA AGAAACATCA AGATGACCAG 
CCAGGCCAAG GTGAAAAATC CCTTGCAAGA AACATCTCTC TAAAAAAGGC AAAGAAGCCG 
CCTTTACCTC CGTCTAGAAC AGACTCACTC AGAAGGGTAC CCAAGAAGAA AGCTCAGTCC 
AATGGACAGG TACTTGATGA AACCCTCATT GCTACCCTCC AGCATTCTCT GCAGTTGAAT 
CTCAAGTGCA AAAATGGCAG CTCCCCTTCC CAGAGCCCCT GCAGTGACTA TGAGGATCCC 
TGGGTGTTGC GCTCTCGCAG CCAAAGCTCG GTCAGCGCAA GCAGCAGCAT GATGTCCACT 
ACTGCCCCAA ACCTGTACTC CATTTGCACA GTCACTCCCT CTCAGAGTGA AACAAGCAGC 
ATCAAGTCAG AGTATGCTGA CCAGTGGGGT TACTACAGTG ACTATGCTGC AGTGGCAGAT 
GACCAGATGA AGTCTCCAGT GACCCATTCT GCTAGTACCT CTTCTGCTCT CAGTGATTAC 
AGCATCAGCC ACTTCAGTGA TGGCTCAAGG GCTTCTGTAC CACAAGTGCC CGCTGGACTG 
GCCAAACCAA AGAGCACTTC TCCAGAGAAG TCCCACCGAG TCACATCGCC ATCCAGTGGG 
TACTCCAGCC AGTCCAATAC ACCCACTGCA CTCACTCCAG TGCCTGTGTT TTTAAAATCT 
GCGTCATCAG GAAATGGGAA ATCCAAGCTG AAACCTAAAG TGCCTGAAAG GAAATCTTCT 
CTTCTGTCTT CAGTCTCCAT TTCTTCATCC TCTACTTCTC TTTCTTCAAA TACATCTACT 
GAAGGGAGTG TGAATGTGAA GAAATTGGAC CCCACCCCGA GTTCTCCTCC AGATTCTGGT 
GCACCTCTTC CTCCGCCTCC TCTTCTAGCA CCTCCTCCAC ATTGCCTTGA GCTCTCTCCT 
CCCTCTTCCC CTCCTCCTCC TCCAGCAGAA GCCGTGGATA TATCACCATT GCCAGCTTCT 
CCCACATTCC CCCCCCCTCC ACCAGAAGCT GATGTAAATT CTTCCTTTGC TCACAGTGTC 
CAGTGGTTAC CACAAGAAGC TTCCATCGGT TCGTACTCTT CCCCTGTTCC TCCTCCTGTT 
TTCCCTTTAG TTGTCCCACC ACCAGCCCCA CCTCTTGATC CCAAACTGAC AAAAGGTGCA 
ATGATATGCC CACAGTATTC ATTTAAGAAA CATGATCAGG AAGATTCTTG CTACAATGCT 
GTGAAACAGC CATTCAGCAA GCAAGATGCA TCAAGACCTG TGATGCCCTT GGTAACCACC 
AAAGCATTGC AAATGGTGCA GCTGAGGTCT ATCAGAAAAG CAACAGAAGG TGACCCATCA 
CCTGAATCTG CTTCTGAAAC CATCTCTCAG GAAAAGGGTA TTGTCACTTC ATCATCACAG 
TCTTCCCTAA GGCCATCTCT CTCACTACGA CTCAGTAACA GCTTAGGTGA AGAGGAGATG 
AAGATTCATG GCACTTCATT TAAAAACACA CCTCAAACAC TGGCTCAGAG TTCTCCTCTG 
GTACTCTCTG ACAACGTACC CATGCTTGAG AGCGATCAAA AAGCTGCAGT TACTATGGGT 
CTAAGCAAGT CTTTGGAACC AGATTCCCCA GGGTCAGCTC CAGAAGCCAC ACCCAAGTCC 
CCAGTTCGGA GCGAAGACTT CCATTCTGGC ATGCCACTGC AGGGGTCACC AGCATCTCCA 
GACAAGACTC AGGTCGTTTT GCCAAACAAG AAACCACCTC CCATTTCAAA GAAACCCAAA 
CTGTTCCTTG TTGTACCACC TCCACAGTTA GATCTCACAG TGGAGAAAAT AGCTGAAGTG 
AGTGATACTG TCAGAAGCAC ATCAAGCCCA ACTAAGAGAG ACGCTGTGCT TACACACTGC 
GAGGAGGCGA GAAATTGCCT TACAGATGGA CTCAGCTCCA GCGAGATGGA CTCTGGCAGC 
CTGGTTCCTG AGGGAGGAGC TGCCAGAGTC ACCTTCTCTG AAGCAGTGGG AGTTGGTGCC 
TTTGTGGTAC AGTCCGCTGC ATCACCAGTT CAAGAGGAGC CCAGGCAAGA GGAGCAGTCA 
GTTTTTGATG AAGGAAGCAG CTCAGGCAGC AGCCAAGACA GCAGCAGTAA CACTGAAGGA 
CACCCGTCTC AGGAGAACGA AAGTGCCGAG GTGTTTGAAT CAGAAACAGC AAATAGTTCA 
TTCCTGCCAA GCAATAATTA TGGGGAAGAG ACGGATGGAG TGGCAACACC AGCGAGACCG 
AGGACTACTG AGGATCTTTT TGCAGCCATT CACAGATCCA AAAGGAAAGT CCTTGGCCGT 
AAGGATTCTG AGGACGATCG TACCCGCAAC CATTCTCCTT CACCCCCCGT AACACCCACT 
GGTGCTTCCC CAACCTTATC TACCCTCAAA CAGGCAGGAT CTATTCAGAG AAGCGTTCGC 
AAGAGCAGCA CCAGCAATGA CAACTTCAAA GCCCTGCTGC TGAAGAAAGG GAGTCGCTGT 
GACACCAGTT CCCGCATGTC AGCAGCAGAG ATGTTGAAGC ACACGGATCC ACGGTTCCAC 
CGAGCCAAGG TGGATGCCTC TCCCGACCTT TCCGACAGCC CTGCCAGCTG CTCGCCCAGC 
AAGAGCAAAC GGGCCCAAGA GGAGTGGGCC AAGAGTGAAG GCCTGATGCC GAGGAGCATG 
TCCCTCTCCA GCACTAGGTA TGGCCGATCA CGAACACCGC CCTCCGCTGC CAGCAGCAAG 
TACAATGTTC GCAACCGCAT CCAGAGCAGC CCCATGACAG TGATTAGCGA AGGAGATGGG 
GAAGTGGTGG AGCAGACAGA GAGCAGGGTC CAAAGGACTT TGGATGAGCA GCGAGAGAGG 
CAGCTCGATG TATTTAACAG TGATGAAATA GACATGAATG ACTTTTCGTA TTCTGAGGAG 
CTGGACTGCA AGGAGACCTT GGATCCAACC CATCTGGACT TAATGACTCA GCCGGGTACT 
TCGAGAAAGT ACCTAAGTCC TTCAGCTGAA GAAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_419717.6 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_419717.3 Gallus gallus NHS like 1 (NHSL1), transcript variant X10, mRNA.

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CloneID	OGa06410
Clone ID Related Accession (Same CDS sequence)	XM_419717.6 , XM_419717.5
Accession Version	XM_419717.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	NHS-like protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_419717.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGTTT TCATCAACAC GAAGCTTAAA TCTCTCATGA AACTTTTCAA GAGAAAGACT 
GTTTCCAACT TAGATGAAGA GAGCAGATGG ACCGTCCATT ACACTGCTCC ATGGCACCAG 
CAGGAAAATG TGTTCCTGCC TTCCTCAAGG CCGCCTTGTG TGGAGGACTT GCACCGGCAA 
GCAAAGCTGA ACCTCAAGTC AGTACTGAGA GAGTGTGACA AGCTGCGGAG AGATGGCTAT 
CGGAGCTCTC AGTATTACTC TCAGGGCCCC ACCTTCTCCT CATCCTCCAG TGCAATCTGT 
GGAAGTTACC AGGACGATTA TGAAGAAATT GAACAAAAGT GTTCTGTCTC TTCCCCAGAA 
GAAGAAAAGC TTATCACCAT CAAAAGGCCT AAAACCCCAG TTTCAAATGA CTTATCAGAT 
ATCAACACAC AAACCAACTG GACTAAGTCA CTTCCACTGC CAACACCAGA AGAGAAGATG 
CGACAGCAAG CACAGGCAGT CCAAACAGAT GTGGTTCCTA TCAATGTGAC TGGGGAGAAT 
TTCGACCGCC AAGCCAGCAT TCGGCGGTCT CTAATTTACA CAGACACGGT GGTAAGACGG 
CCGAAGAAAG TCAAAAGGAG AAAGACTATA ACAGGAATCC CTGACAACAT ACAAAAGGAG 
CTAGCAGTTG GCACAGGCCA AAGTGATTTC CGAGGGCACT CGATGTATGT CCCAGATCAC 
AGTTCCACGC TAGGGCGACT AGACAGCTAT CGCTCTGCTA TGCAGCGGTC TGAAACCAAG 
GACACCAGCT GCCAGACTGA AGAAGTTAAA GTTGTGCCTC CTTCGATGAG AAGAATACGA 
GCACAGAAAG GACAAGGCAT TGCAGCTCAG ATGTCTCAGT TCTCCAGCTC ATCTGGAAAC 
ATGTCTGTGA TGAGTGATTC TGCTGCAGTT ATATTTGCTT CTCGCCAGAA TAGTGACATA 
GGTTTTCACA GCTTGCCTCG GGCTGGTGCA AGAGTGTCTC TGCAGTCCTT AGAACAGACC 
CAGAGCATCT CCAGACAGAC AGAAGACATT GCTGGTACTT TGCCTCACCA GATGAGTAAA 
CTGCAAGTGG ATGATGATGT TGCACATCTG AGAAATAATG CCATAACAGG AACTGTTTCG 
AGGCCAAAGT CTCAAGAGGT AAGAAGTTGT GACAGTGAAA AGTCCACTAG CCCAGCATGT 
GTGGTCTCTC CTCATGCCAC CTACTCAACA AGCGTCATAC CCAATGCGAC ACTGTCATCC 
TCCTCAGAAG TTATTGCTAT TCACACTGCC CAGAGTACTG GATCGCTGGA TAACAAAATT 
AGCAGTTCTG CTGTGTACCC AAAACCAAGA GATAATCCTG TTGCCAACAA TGCAATAAGT 
GGGAAAGAAG ATCACCATTC TTCAAGTGGT AACTGGAGTG AGAGTAGCTC CACACGTCAC 
TCACAGACTT CAGATACCAT CCCATCTCAT ACTGTCATGA TGCTTTCTCT TGGTGACTCT 
GCTGTCTCTC TCAGCACTCC TGGAAATGTA GAGGCTGGCT CTCAGAGTGT GAACTACAGC 
TGCAGAAGCA ATCTTGCTTC ACCAAACCAG TCCCAGGACA GCGATGGTAG GAGTGAATCT 
AGCTACTCAG GGGAGCGAAC ACAAGGTGTA GTGAGCAGCA CAGAGCACTG GCTATACAAG 
TCTGCGGAAA ATAGTGAGAC TGCATCATGC AAGGTAGCTT GTACCACACC AGGCTGTGCC 
ACTCCTGGTA GCAACCTGAG CAGCAGCAGC CTGGAGAGGA CATCAGCCAA AGAAGATTCC 
ACTTCACTGT ATTCTGTAGA CCACGATGGT TATTACAGCT CTATGAGCAT GGACTCTGGA 
CTGAAGTCAG ACATGCCATG CTATAGTACT AATGGTTTTG GGAACCCCAG AGACAGCGTG 
ATTAATGTCT TTGAAGCAAA GGAGAAGAAA CATCAAGATG ACCAGCCAGG CCAAGGTGAA 
AAATCCCTTG CAAGAAACAT CTCTCTAAAA AAGGCAAAGA AGCCGCCTTT ACCTCCGTCT 
AGAACAGACT CACTCAGAAG GGTACCCAAG AAGAAAGCTC AGTCCAATGG ACAGGTACTT 
GATGAAACCC TCATTGCTAC CCTCCAGCAT TCTCTGCAGT TGAATCTCAA GTGCAAAAAT 
GGCAGCTCCC CTTCCCAGAG CCCCTGCAGT GACTATGAGG ATCCCTGGGT GTTGCGCTCT 
CGCAGCCAAA GCTCGGTCAG CGCAAGCAGC AGCATGATGT CCACTACTGC CCCAAACCTG 
TACTCCATTT GCACAGTCAC TCCCTCTCAG AGTGAAACAA GCAGCATCAA GTCAGAGTAT 
GCTGACCAGT GGGGTTACTA CAGTGACTAT GCTGCAGTGG CAGATGACCA GATGAAGTCT 
CCAGTGACCC ATTCTGCTAG TACCTCTTCT GCTCTCAGTG ATTACAGCAT CAGCCACTTC 
AGTGATGGCT CAAGGGCTTC TGTACCACAA GTGCCCGCTG GACTGGCCAA ACCAAAGAGC 
ACTTCTCCAG AGAAGTCCCA CCGAGTCACA TCGCCATCCA GTGGGTACTC CAGCCAGTCC 
AATACACCCA CTGCACTCAC TCCAGTGCCT GTGTTTTTAA AATCTGCGTC ATCAGGAAAT 
GGGAAATCCA AGCTGAAACC TAAAGTGCCT GAAAGGAAAT CTTCTCTTCT GTCTTCAGTC 
TCCATTTCTT CATCCTCTAC TTCTCTTTCT TCAAATACAT CTACTGAAGG GAGTGTGAAT 
GTGAAGAAAT TGGACCCCAC CCCGAGTTCT CCTCCAGATT CTGGTGCACC TCTTCCTCCG 
CCTCCTCTTC TAGCACCTCC TCCACATTGC CTTGAGCTCT CTCCTCCCTC TTCCCCTCCT 
CCTCCTCCAG CAGAAGCCGT GGATATATCA CCATTGCCAG CTTCTCCCAC ATTCCCCCCC 
CCTCCACCAG AAGCTGATGT AAATTCTTCC TTTGCTCACA GTGTCCAGTG GTTACCACAA 
GAAGCTTCCA TCGGTTCGTA CTCTTCCCCT GTTCCTCCTC CTGTTTTCCC TTTAGTTGTC 
CCACCACCAG CCCCACCTCT TGATCCCAAA CTGACAAAAG GTGCAATGAT ATGCCCACAG 
TATTCATTTA AGAAACATGA TCAGGAAGAT TCTTGCTACA ATGCTGTGAA ACAGCCATTC 
AGCAAGCAAG ATGCATCAAG ACCTGTGATG CCCTTGGTAA CCACCAAAGC ATTGCAAATG 
GTGCAGCTGA GGTCTATCAG AAAAGCAACA GAAGGTGACC CATCACCTGA ATCTGCTTCT 
GAAACCATCT CTCAGGAAAA GGGTATTGTC ACTTCATCAT CACAGTCTTC CCTAAGGCCA 
TCTCTCTCAC TACGACTCAG TAACAGCTTA GGTGAAGAGG AGATGAAGAT TCATGGCACT 
TCATTTAAAA ACACACCTCA AACACTGGCT CAGAGTTCTC CTCTGGTACT CTCTGACAAC 
GTACCCATGC TTGAGAGCGA TCAAAAAGCT GCAGTTACTA TGGGTCTAAG CAAGTCTTTG 
GAACCAGATT CCCCAGGGTC AGCTCCAGAA GCCACACCCA AGTCCCCAGT TCGGAGCGAA 
GACTTCCATT CTGGCATGCC ACTGCAGGGG TCACCAGCAT CTCCAGACAA GACTCAGGTC 
GTTTTGCCAA ACAAGAAACC ACCTCCCATT TCAAAGAAAC CCAAACTGTT CCTTGTTGTA 
CCACCTCCAC AGTTAGATCT CACAGTGGAG AAAATAGCTG AAGTGAGTGA TACTGTCAGA 
AGCACATCAA GCCCAACTAA GAGAGACGCT GTGCTTACAC ACTGCGAGGA GGCGAGAAAT 
TGCCTTACAG ATGGACTCAG CTCCAGCGAG ATGGACTCTG GCAGCCTGGT TCCTGAGGGA 
GGAGCTGCCA GAGTCACCTT CTCTGAAGCA GTGGGAGTTG GTGCCTTTGT GGTACAGTCC 
GCTGCATCAC CAGTTCAAGA GGAGCCCAGG CAAGAGGAGC AGTCAGTTTT TGATGAAGGA 
AGCAGCTCAG GCAGCAGCCA AGACAGCAGC AGTAACACTG AAGGACACCC GTCTCAGGAG 
AACGAAAGTG CCGAGGTGTT TGAATCAGAA ACAGCAAATA GTTCATTCCT GCCAAGCAAT 
AATTATGGGG AAGAGACGGA TGGAGTGGCA ACACCAGCGA GACCGAGGAC TACTGAGGAT 
CTTTTTGCAG CCATTCACAG ATCCAAAAGG AAAGTCCTTG GCCGTAAGGA TTCTGAGGAC 
GATCGTACCC GCAACCATTC TCCTTCACCC CCCGTAACAC CCACTGGTGC TTCCCCAACC 
TTATCTACCC TCAAACAGGC AGGATCTATT CAGAGAAGCG TTCGCAAGAG CAGCACCAGC 
AATGACAACT TCAAAGCCCT GCTGCTGAAG AAAGGGAGTC GCTGTGACAC CAGTTCCCGC 
ATGTCAGCAG CAGAGATGTT GAAGCACACG GATCCACGGT TCCACCGAGC CAAGGTGGAT 
GCCTCTCCCG ACCTTTCCGA CAGCCCTGCC AGCTGCTCGC CCAGCAAGAG CAAACGGGCC 
CAAGAGGAGT GGGCCAAGAG TGAAGGCCTG ATGCCGAGGA GCATGTCCCT CTCCAGCACT 
AGGTATGGCC GATCACGAAC ACCGCCCTCC GCTGCCAGCA GCAAGTACAA TGTTCGCAAC 
CGCATCCAGA GCAGCCCCAT GACAGTGATT AGCGAAGGAG ATGGGGAAGT GGTGGAGCAG 
ACAGAGAGCA GGGTCCAAAG GACTTTGGAT GAGCAGCGAG AGAGGCAGCT CGATGTATTT 
AACAGTGATG AAATAGACAT GAATGACTTT TCGTATTCTG AGGAGCTGGA CTGCAAGGAG 
ACCTTGGATC CAACCCATCT GGACTTAATG ACTCAGCCGG GTACTTCGAG AAAGTACCTA 
AGTCCTTCAG CTGAAGAAAG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_419717.3
CDS	447..5390
Translation	MVVFINTKLKSLMKLFKRKTVSNLDEESRWTVHYTAPWHQQENVFLPSSRPPCVEDLHRQAKLNLKSVLRECDKLRRDGYRSSQYYSQGPTFSSSSSAICGSYQDDYEEIEQKCSVSSPEEEKLITIKRPKTPVSNDLSDINTQTNWTKSLPLPTPEEKMRQQAQAVQTDVVPINVTGENFDRQASIRRSLIYTDTVVRRPKKVKRRKTITGIPDNIQKELAVGTGQSDFRGHSMYVPDHSSTLGRLDSYRSAMQRSETKDTSCQTEEVKVVPPSMRRIRAQKGQGIAAQMSQFSSSSGNMSVMSDSAAVIFASRQNSDIGFHSLPRAGARVSLQSLEQTQSISRQTEDIAGTLPHQMSKLQVDDDVAHLRNNAITGTVSRPKSQEVRSCDSEKSTSPACVVSPHATYSTSVIPNATLSSSSEVIAIHTAQSTGSLDNKISSSAVYPKPRDNPVANNAISGKEDHHSSSGNWSESSSTRHSQTSDTIPSHTVMMLSLGDSAVSLSTPGNVEAGSQSVNYSCRSNLASPNQSQDSDGRSESSYSGERTQGVVSSTEHWLYKSAENSETASCKVACTTPGCATPGSNLSSSSLERTSAKEDSTSLYSVDHDGYYSSMSMDSGLKSDMPCYSTNGFGNPRDSVINVFEAKEKKHQDDQPGQGEKSLARNISLKKAKKPPLPPSRTDSLRRVPKKKAQSNGQVLDETLIATLQHSLQLNLKCKNGSSPSQSPCSDYEDPWVLRSRSQSSVSASSSMMSTTAPNLYSICTVTPSQSETSSIKSEYADQWGYYSDYAAVADDQMKSPVTHSASTSSALSDYSISHFSDGSRASVPQVPAGLAKPKSTSPEKSHRVTSPSSGYSSQSNTPTALTPVPVFLKSASSGNGKSKLKPKVPERKSSLLSSVSISSSSTSLSSNTSTEGSVNVKKLDPTPSSPPDSGAPLPPPPLLAPPPHCLELSPPSSPPPPPAEAVDISPLPASPTFPPPPPEADVNSSFAHSVQWLPQEASIGSYSSPVPPPVFPLVVPPPAPPLDPKLTKGAMICPQYSFKKHDQEDSCYNAVKQPFSKQDASRPVMPLVTTKALQMVQLRSIRKATEGDPSPESASETISQEKGIVTSSSQSSLRPSLSLRLSNSLGEEEMKIHGTSFKNTPQTLAQSSPLVLSDNVPMLESDQKAAVTMGLSKSLEPDSPGSAPEATPKSPVRSEDFHSGMPLQGSPASPDKTQVVLPNKKPPPISKKPKLFLVVPPPQLDLTVEKIAEVSDTVRSTSSPTKRDAVLTHCEEARNCLTDGLSSSEMDSGSLVPEGGAARVTFSEAVGVGAFVVQSAASPVQEEPRQEEQSVFDEGSSSGSSQDSSSNTEGHPSQENESAEVFESETANSSFLPSNNYGEETDGVATPARPRTTEDLFAAIHRSKRKVLGRKDSEDDRTRNHSPSPPVTPTGASPTLSTLKQAGSIQRSVRKSSTSNDNFKALLLKKGSRCDTSSRMSAAEMLKHTDPRFHRAKVDASPDLSDSPASCSPSKSKRAQEEWAKSEGLMPRSMSLSSTRYGRSRTPPSAASSKYNVRNRIQSSPMTVISEGDGEVVEQTESRVQRTLDEQRERQLDVFNSDEIDMNDFSYSEELDCKETLDPTHLDLMTQPGTSRKYLSPSAEES

Target ORF information:

RefSeq Version	XM_419717.6
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NHS like 1 (NHSL1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_419717.6

ORF Insert Sequence:

ATGGTGGTTT TCATCAACAC GAAGCTTAAA TCTCTCATGA AACTTTTCAA GAGAAAGACT 
GTTTCCAACT TAGATGAAGA GAGCAGATGG ACCGTCCATT ACACTGCTCC ATGGCACCAG 
CAGGAAAATG TGTTCCTGCC TTCCTCAAGG CCGCCTTGTG TGGAGGACTT GCACCGGCAA 
GCAAAGCTGA ACCTCAAGTC AGTACTGAGA GAGTGTGACA AGCTGCGGAG AGATGGCTAT 
CGGAGCTCTC AGTATTACTC TCAGGGCCCC ACCTTCTCCT CATCCTCCAG TGCAATCTGT 
GGAAGTTACC AGGACGATTA TGAAGAAATT GAACAAAAGT GTTCTGTCTC TTCCCCAGAA 
GAAGAAAAGC TTATCACCAT CAAAAGGCCT AAAACCCCAG TTTCAAATGA CTTATCAGAT 
ATCAACACAC AAACCAACTG GACTAAGTCA CTTCCACTGC CAACACCAGA AGAGAAGATG 
CGACAGCAAG CACAGGCAGT CCAAACAGAT GTGGTTCCTA TCAATGTGAC TGGGGAGAAT 
TTCGACCGCC AAGCCAGCAT TCGGCGGTCT CTAATTTACA CAGACACGGT GGTAAGACGG 
CCGAAGAAAG TCAAAAGGAG AAAGACTATA ACAGGAATCC CTGACAACAT ACAAAAGGAG 
CTAGCAGTTG GCACAGGCCA AAGTGATTTC CGAGGGCACT CGATGTATGT CCCAGATCAC 
AGTTCCACGC TAGGGCGACT AGACAGCTAT CGCTCTGCTA TGCAGCGGTC TGAAACCAAG 
GACACCAGCT GCCAGACTGA AGAAGTTAAA GTTGTGCCTC CTTCGATGAG AAGAATACGA 
GCACAGAAAG GACAAGGCAT TGCAGCTCAG ATGTCTCAGT TCTCCAGCTC ATCTGGAAAC 
ATGTCTGTGA TGAGTGATTC TGCTGCAGTT ATATTTGCTT CTCGCCAGAA TAGTGACATA 
GGTTTTCACA GCTTGCCTCG GGCTGGTGCA AGAGTGTCTC TGCAGTCCTT AGAACAGACC 
CAGAGCATCT CCAGACAGAC AGAAGACATT GCTGGTACTT TGCCTCACCA GATGAGTAAA 
CTGCAAGTGG ATGATGATGT TGCACATCTG AGAAATAATG CCATAACAGG AACTGTTTCG 
AGGCCAAAGT CTCAAGAGGT AAGAAGTTGT GACAGTGAAA AGTCCACTAG CCCAGCATGT 
GTGGTCTCTC CTCATGCCAC CTACTCAACA AGCGTCATAC CCAATGCGAC ACTGTCATCC 
TCCTCAGAAG TTATTGCTAT TCACACTGCC CAGAGTACTG GATCGCTGGA TAACAAAATT 
AGCAGTTCTG CTGTGTACCC AAAACCAAGA GATAATCCTG TTGCCAACAA TGCAATAAGT 
GGGAAAGAAG ATCACCATTC TTCAAGTGGT AACTGGAGTG AGAGTAGCTC CACACGTCAC 
TCACAGACTT CAGATACCAT CCCATCTCAT ACTGTCATGA TGCTTTCTCT TGGTGACTCT 
GCTGTCTCTC TCAGCACTCC TGGAAATGTA GAGGCTGGCT CTCAGAGTGT GAACTACAGC 
TGCAGAAGCA ATCTTGCTTC ACCAAACCAG TCCCAGGACA GCGATGGTAG GAGTGAATCT 
AGCTACTCAG GGGAGCGAAC ACAAGGTGTA GTGAGCAGCA CAGAGCACTG GCTATACAAG 
TCTGCGGAAA ATAGTGAGAC TGCATCATGC AAGGTAGCTT GTACCACACC AGGCTGTGCC 
ACTCCTGGTA GCAACCTGAG CAGCAGCAGC CTGGAGAGGA CATCAGCCAA AGAAGATTCC 
ACTTCACTGT ATTCTGTAGA CCACGATGGT TATTACAGCT CTATGAGCAT GGACTCTGGA 
CTGAAGTCAG ACATGCCATG CTATAGTACT AATGGTTTTG GGAACCCCAG AGACAGCGTG 
ATTAATGTCT TTGAAGCAAA GGAGAAGAAA CATCAAGATG ACCAGCCAGG CCAAGGTGAA 
AAATCCCTTG CAAGAAACAT CTCTCTAAAA AAGGCAAAGA AGCCGCCTTT ACCTCCGTCT 
AGAACAGACT CACTCAGAAG GGTACCCAAG AAGAAAGCTC AGTCCAATGG ACAGGTACTT 
GATGAAACCC TCATTGCTAC CCTCCAGCAT TCTCTGCAGT TGAATCTCAA GTGCAAAAAT 
GGCAGCTCCC CTTCCCAGAG CCCCTGCAGT GACTATGAGG ATCCCTGGGT GTTGCGCTCT 
CGCAGCCAAA GCTCGGTCAG CGCAAGCAGC AGCATGATGT CCACTACTGC CCCAAACCTG 
TACTCCATTT GCACAGTCAC TCCCTCTCAG AGTGAAACAA GCAGCATCAA GTCAGAGTAT 
GCTGACCAGT GGGGTTACTA CAGTGACTAT GCTGCAGTGG CAGATGACCA GATGAAGTCT 
CCAGTGACCC ATTCTGCTAG TACCTCTTCT GCTCTCAGTG ATTACAGCAT CAGCCACTTC 
AGTGATGGCT CAAGGGCTTC TGTACCACAA GTGCCCGCTG GACTGGCCAA ACCAAAGAGC 
ACTTCTCCAG AGAAGTCCCA CCGAGTCACA TCGCCATCCA GTGGGTACTC CAGCCAGTCC 
AATACACCCA CTGCACTCAC TCCAGTGCCT GTGTTTTTAA AATCTGCGTC ATCAGGAAAT 
GGGAAATCCA AGCTGAAACC TAAAGTGCCT GAAAGGAAAT CTTCTCTTCT GTCTTCAGTC 
TCCATTTCTT CATCCTCTAC TTCTCTTTCT TCAAATACAT CTACTGAAGG GAGTGTGAAT 
GTGAAGAAAT TGGACCCCAC CCCGAGTTCT CCTCCAGATT CTGGTGCACC TCTTCCTCCG 
CCTCCTCTTC TAGCACCTCC TCCACATTGC CTTGAGCTCT CTCCTCCCTC TTCCCCTCCT 
CCTCCTCCAG CAGAAGCCGT GGATATATCA CCATTGCCAG CTTCTCCCAC ATTCCCCCCC 
CCTCCACCAG AAGCTGATGT AAATTCTTCC TTTGCTCACA GTGTCCAGTG GTTACCACAA 
GAAGCTTCCA TCGGTTCGTA CTCTTCCCCT GTTCCTCCTC CTGTTTTCCC TTTAGTTGTC 
CCACCACCAG CCCCACCTCT TGATCCCAAA CTGACAAAAG GTGCAATGAT ATGCCCACAG 
TATTCATTTA AGAAACATGA TCAGGAAGAT TCTTGCTACA ATGCTGTGAA ACAGCCATTC 
AGCAAGCAAG ATGCATCAAG ACCTGTGATG CCCTTGGTAA CCACCAAAGC ATTGCAAATG 
GTGCAGCTGA GGTCTATCAG AAAAGCAACA GAAGGTGACC CATCACCTGA ATCTGCTTCT 
GAAACCATCT CTCAGGAAAA GGGTATTGTC ACTTCATCAT CACAGTCTTC CCTAAGGCCA 
TCTCTCTCAC TACGACTCAG TAACAGCTTA GGTGAAGAGG AGATGAAGAT TCATGGCACT 
TCATTTAAAA ACACACCTCA AACACTGGCT CAGAGTTCTC CTCTGGTACT CTCTGACAAC 
GTACCCATGC TTGAGAGCGA TCAAAAAGCT GCAGTTACTA TGGGTCTAAG CAAGTCTTTG 
GAACCAGATT CCCCAGGGTC AGCTCCAGAA GCCACACCCA AGTCCCCAGT TCGGAGCGAA 
GACTTCCATT CTGGCATGCC ACTGCAGGGG TCACCAGCAT CTCCAGACAA GACTCAGGTC 
GTTTTGCCAA ACAAGAAACC ACCTCCCATT TCAAAGAAAC CCAAACTGTT CCTTGTTGTA 
CCACCTCCAC AGTTAGATCT CACAGTGGAG AAAATAGCTG AAGTGAGTGA TACTGTCAGA 
AGCACATCAA GCCCAACTAA GAGAGACGCT GTGCTTACAC ACTGCGAGGA GGCGAGAAAT 
TGCCTTACAG ATGGACTCAG CTCCAGCGAG ATGGACTCTG GCAGCCTGGT TCCTGAGGGA 
GGAGCTGCCA GAGTCACCTT CTCTGAAGCA GTGGGAGTTG GTGCCTTTGT GGTACAGTCC 
GCTGCATCAC CAGTTCAAGA GGAGCCCAGG CAAGAGGAGC AGTCAGTTTT TGATGAAGGA 
AGCAGCTCAG GCAGCAGCCA AGACAGCAGC AGTAACACTG AAGGACACCC GTCTCAGGAG 
AACGAAAGTG CCGAGGTGTT TGAATCAGAA ACAGCAAATA GTTCATTCCT GCCAAGCAAT 
AATTATGGGG AAGAGACGGA TGGAGTGGCA ACACCAGCGA GACCGAGGAC TACTGAGGAT 
CTTTTTGCAG CCATTCACAG ATCCAAAAGG AAAGTCCTTG GCCGTAAGGA TTCTGAGGAC 
GATCGTACCC GCAACCATTC TCCTTCACCC CCCGTAACAC CCACTGGTGC TTCCCCAACC 
TTATCTACCC TCAAACAGGC AGGATCTATT CAGAGAAGCG TTCGCAAGAG CAGCACCAGC 
AATGACAACT TCAAAGCCCT GCTGCTGAAG AAAGGGAGTC GCTGTGACAC CAGTTCCCGC 
ATGTCAGCAG CAGAGATGTT GAAGCACACG GATCCACGGT TCCACCGAGC CAAGGTGGAT 
GCCTCTCCCG ACCTTTCCGA CAGCCCTGCC AGCTGCTCGC CCAGCAAGAG CAAACGGGCC 
CAAGAGGAGT GGGCCAAGAG TGAAGGCCTG ATGCCGAGGA GCATGTCCCT CTCCAGCACT 
AGGTATGGCC GATCACGAAC ACCGCCCTCC GCTGCCAGCA GCAAGTACAA TGTTCGCAAC 
CGCATCCAGA GCAGCCCCAT GACAGTGATT AGCGAAGGAG ATGGGGAAGT GGTGGAGCAG 
ACAGAGAGCA GGGTCCAAAG GACTTTGGAT GAGCAGCGAG AGAGGCAGCT CGATGTATTT 
AACAGTGATG AAATAGACAT GAATGACTTT TCGTATTCTG AGGAGCTGGA CTGCAAGGAG 
ACCTTGGATC CAACCCATCT GGACTTAATG ACTCAGCCGG GTACTTCGAG AAAGTACCTA 
AGTCCTTCAG CTGAAGAAAG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_419717.5 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_419717.3 Gallus gallus NHS-like 1 (NHSL1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OGa06410
Clone ID Related Accession (Same CDS sequence)	XM_419717.6 , XM_419717.5
Accession Version	XM_419717.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	NHS-like protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455837.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_419717.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGTTT TCATCAACAC GAAGCTTAAA TCTCTCATGA AACTTTTCAA GAGAAAGACT 
GTTTCCAACT TAGATGAAGA GAGCAGATGG ACCGTCCATT ACACTGCTCC ATGGCACCAG 
CAGGAAAATG TGTTCCTGCC TTCCTCAAGG CCGCCTTGTG TGGAGGACTT GCACCGGCAA 
GCAAAGCTGA ACCTCAAGTC AGTACTGAGA GAGTGTGACA AGCTGCGGAG AGATGGCTAT 
CGGAGCTCTC AGTATTACTC TCAGGGCCCC ACCTTCTCCT CATCCTCCAG TGCAATCTGT 
GGAAGTTACC AGGACGATTA TGAAGAAATT GAACAAAAGT GTTCTGTCTC TTCCCCAGAA 
GAAGAAAAGC TTATCACCAT CAAAAGGCCT AAAACCCCAG TTTCAAATGA CTTATCAGAT 
ATCAACACAC AAACCAACTG GACTAAGTCA CTTCCACTGC CAACACCAGA AGAGAAGATG 
CGACAGCAAG CACAGGCAGT CCAAACAGAT GTGGTTCCTA TCAATGTGAC TGGGGAGAAT 
TTCGACCGCC AAGCCAGCAT TCGGCGGTCT CTAATTTACA CAGACACGGT GGTAAGACGG 
CCGAAGAAAG TCAAAAGGAG AAAGACTATA ACAGGAATCC CTGACAACAT ACAAAAGGAG 
CTAGCAGTTG GCACAGGCCA AAGTGATTTC CGAGGGCACT CGATGTATGT CCCAGATCAC 
AGTTCCACGC TAGGGCGACT AGACAGCTAT CGCTCTGCTA TGCAGCGGTC TGAAACCAAG 
GACACCAGCT GCCAGACTGA AGAAGTTAAA GTTGTGCCTC CTTCGATGAG AAGAATACGA 
GCACAGAAAG GACAAGGCAT TGCAGCTCAG ATGTCTCAGT TCTCCAGCTC ATCTGGAAAC 
ATGTCTGTGA TGAGTGATTC TGCTGCAGTT ATATTTGCTT CTCGCCAGAA TAGTGACATA 
GGTTTTCACA GCTTGCCTCG GGCTGGTGCA AGAGTGTCTC TGCAGTCCTT AGAACAGACC 
CAGAGCATCT CCAGACAGAC AGAAGACATT GCTGGTACTT TGCCTCACCA GATGAGTAAA 
CTGCAAGTGG ATGATGATGT TGCACATCTG AGAAATAATG CCATAACAGG AACTGTTTCG 
AGGCCAAAGT CTCAAGAGGT AAGAAGTTGT GACAGTGAAA AGTCCACTAG CCCAGCATGT 
GTGGTCTCTC CTCATGCCAC CTACTCAACA AGCGTCATAC CCAATGCGAC ACTGTCATCC 
TCCTCAGAAG TTATTGCTAT TCACACTGCC CAGAGTACTG GATCGCTGGA TAACAAAATT 
AGCAGTTCTG CTGTGTACCC AAAACCAAGA GATAATCCTG TTGCCAACAA TGCAATAAGT 
GGGAAAGAAG ATCACCATTC TTCAAGTGGT AACTGGAGTG AGAGTAGCTC CACACGTCAC 
TCACAGACTT CAGATACCAT CCCATCTCAT ACTGTCATGA TGCTTTCTCT TGGTGACTCT 
GCTGTCTCTC TCAGCACTCC TGGAAATGTA GAGGCTGGCT CTCAGAGTGT GAACTACAGC 
TGCAGAAGCA ATCTTGCTTC ACCAAACCAG TCCCAGGACA GCGATGGTAG GAGTGAATCT 
AGCTACTCAG GGGAGCGAAC ACAAGGTGTA GTGAGCAGCA CAGAGCACTG GCTATACAAG 
TCTGCGGAAA ATAGTGAGAC TGCATCATGC AAGGTAGCTT GTACCACACC AGGCTGTGCC 
ACTCCTGGTA GCAACCTGAG CAGCAGCAGC CTGGAGAGGA CATCAGCCAA AGAAGATTCC 
ACTTCACTGT ATTCTGTAGA CCACGATGGT TATTACAGCT CTATGAGCAT GGACTCTGGA 
CTGAAGTCAG ACATGCCATG CTATAGTACT AATGGTTTTG GGAACCCCAG AGACAGCGTG 
ATTAATGTCT TTGAAGCAAA GGAGAAGAAA CATCAAGATG ACCAGCCAGG CCAAGGTGAA 
AAATCCCTTG CAAGAAACAT CTCTCTAAAA AAGGCAAAGA AGCCGCCTTT ACCTCCGTCT 
AGAACAGACT CACTCAGAAG GGTACCCAAG AAGAAAGCTC AGTCCAATGG ACAGGTACTT 
GATGAAACCC TCATTGCTAC CCTCCAGCAT TCTCTGCAGT TGAATCTCAA GTGCAAAAAT 
GGCAGCTCCC CTTCCCAGAG CCCCTGCAGT GACTATGAGG ATCCCTGGGT GTTGCGCTCT 
CGCAGCCAAA GCTCGGTCAG CGCAAGCAGC AGCATGATGT CCACTACTGC CCCAAACCTG 
TACTCCATTT GCACAGTCAC TCCCTCTCAG AGTGAAACAA GCAGCATCAA GTCAGAGTAT 
GCTGACCAGT GGGGTTACTA CAGTGACTAT GCTGCAGTGG CAGATGACCA GATGAAGTCT 
CCAGTGACCC ATTCTGCTAG TACCTCTTCT GCTCTCAGTG ATTACAGCAT CAGCCACTTC 
AGTGATGGCT CAAGGGCTTC TGTACCACAA GTGCCCGCTG GACTGGCCAA ACCAAAGAGC 
ACTTCTCCAG AGAAGTCCCA CCGAGTCACA TCGCCATCCA GTGGGTACTC CAGCCAGTCC 
AATACACCCA CTGCACTCAC TCCAGTGCCT GTGTTTTTAA AATCTGCGTC ATCAGGAAAT 
GGGAAATCCA AGCTGAAACC TAAAGTGCCT GAAAGGAAAT CTTCTCTTCT GTCTTCAGTC 
TCCATTTCTT CATCCTCTAC TTCTCTTTCT TCAAATACAT CTACTGAAGG GAGTGTGAAT 
GTGAAGAAAT TGGACCCCAC CCCGAGTTCT CCTCCAGATT CTGGTGCACC TCTTCCTCCG 
CCTCCTCTTC TAGCACCTCC TCCACATTGC CTTGAGCTCT CTCCTCCCTC TTCCCCTCCT 
CCTCCTCCAG CAGAAGCCGT GGATATATCA CCATTGCCAG CTTCTCCCAC ATTCCCCCCC 
CCTCCACCAG AAGCTGATGT AAATTCTTCC TTTGCTCACA GTGTCCAGTG GTTACCACAA 
GAAGCTTCCA TCGGTTCGTA CTCTTCCCCT GTTCCTCCTC CTGTTTTCCC TTTAGTTGTC 
CCACCACCAG CCCCACCTCT TGATCCCAAA CTGACAAAAG GTGCAATGAT ATGCCCACAG 
TATTCATTTA AGAAACATGA TCAGGAAGAT TCTTGCTACA ATGCTGTGAA ACAGCCATTC 
AGCAAGCAAG ATGCATCAAG ACCTGTGATG CCCTTGGTAA CCACCAAAGC ATTGCAAATG 
GTGCAGCTGA GGTCTATCAG AAAAGCAACA GAAGGTGACC CATCACCTGA ATCTGCTTCT 
GAAACCATCT CTCAGGAAAA GGGTATTGTC ACTTCATCAT CACAGTCTTC CCTAAGGCCA 
TCTCTCTCAC TACGACTCAG TAACAGCTTA GGTGAAGAGG AGATGAAGAT TCATGGCACT 
TCATTTAAAA ACACACCTCA AACACTGGCT CAGAGTTCTC CTCTGGTACT CTCTGACAAC 
GTACCCATGC TTGAGAGCGA TCAAAAAGCT GCAGTTACTA TGGGTCTAAG CAAGTCTTTG 
GAACCAGATT CCCCAGGGTC AGCTCCAGAA GCCACACCCA AGTCCCCAGT TCGGAGCGAA 
GACTTCCATT CTGGCATGCC ACTGCAGGGG TCACCAGCAT CTCCAGACAA GACTCAGGTC 
GTTTTGCCAA ACAAGAAACC ACCTCCCATT TCAAAGAAAC CCAAACTGTT CCTTGTTGTA 
CCACCTCCAC AGTTAGATCT CACAGTGGAG AAAATAGCTG AAGTGAGTGA TACTGTCAGA 
AGCACATCAA GCCCAACTAA GAGAGACGCT GTGCTTACAC ACTGCGAGGA GGCGAGAAAT 
TGCCTTACAG ATGGACTCAG CTCCAGCGAG ATGGACTCTG GCAGCCTGGT TCCTGAGGGA 
GGAGCTGCCA GAGTCACCTT CTCTGAAGCA GTGGGAGTTG GTGCCTTTGT GGTACAGTCC 
GCTGCATCAC CAGTTCAAGA GGAGCCCAGG CAAGAGGAGC AGTCAGTTTT TGATGAAGGA 
AGCAGCTCAG GCAGCAGCCA AGACAGCAGC AGTAACACTG AAGGACACCC GTCTCAGGAG 
AACGAAAGTG CCGAGGTGTT TGAATCAGAA ACAGCAAATA GTTCATTCCT GCCAAGCAAT 
AATTATGGGG AAGAGACGGA TGGAGTGGCA ACACCAGCGA GACCGAGGAC TACTGAGGAT 
CTTTTTGCAG CCATTCACAG ATCCAAAAGG AAAGTCCTTG GCCGTAAGGA TTCTGAGGAC 
GATCGTACCC GCAACCATTC TCCTTCACCC CCCGTAACAC CCACTGGTGC TTCCCCAACC 
TTATCTACCC TCAAACAGGC AGGATCTATT CAGAGAAGCG TTCGCAAGAG CAGCACCAGC 
AATGACAACT TCAAAGCCCT GCTGCTGAAG AAAGGGAGTC GCTGTGACAC CAGTTCCCGC 
ATGTCAGCAG CAGAGATGTT GAAGCACACG GATCCACGGT TCCACCGAGC CAAGGTGGAT 
GCCTCTCCCG ACCTTTCCGA CAGCCCTGCC AGCTGCTCGC CCAGCAAGAG CAAACGGGCC 
CAAGAGGAGT GGGCCAAGAG TGAAGGCCTG ATGCCGAGGA GCATGTCCCT CTCCAGCACT 
AGGTATGGCC GATCACGAAC ACCGCCCTCC GCTGCCAGCA GCAAGTACAA TGTTCGCAAC 
CGCATCCAGA GCAGCCCCAT GACAGTGATT AGCGAAGGAG ATGGGGAAGT GGTGGAGCAG 
ACAGAGAGCA GGGTCCAAAG GACTTTGGAT GAGCAGCGAG AGAGGCAGCT CGATGTATTT 
AACAGTGATG AAATAGACAT GAATGACTTT TCGTATTCTG AGGAGCTGGA CTGCAAGGAG 
ACCTTGGATC CAACCCATCT GGACTTAATG ACTCAGCCGG GTACTTCGAG AAAGTACCTA 
AGTCCTTCAG CTGAAGAAAG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_419717.3
CDS	451..5394
Translation	MVVFINTKLKSLMKLFKRKTVSNLDEESRWTVHYTAPWHQQENVFLPSSRPPCVEDLHRQAKLNLKSVLRECDKLRRDGYRSSQYYSQGPTFSSSSSAICGSYQDDYEEIEQKCSVSSPEEEKLITIKRPKTPVSNDLSDINTQTNWTKSLPLPTPEEKMRQQAQAVQTDVVPINVTGENFDRQASIRRSLIYTDTVVRRPKKVKRRKTITGIPDNIQKELAVGTGQSDFRGHSMYVPDHSSTLGRLDSYRSAMQRSETKDTSCQTEEVKVVPPSMRRIRAQKGQGIAAQMSQFSSSSGNMSVMSDSAAVIFASRQNSDIGFHSLPRAGARVSLQSLEQTQSISRQTEDIAGTLPHQMSKLQVDDDVAHLRNNAITGTVSRPKSQEVRSCDSEKSTSPACVVSPHATYSTSVIPNATLSSSSEVIAIHTAQSTGSLDNKISSSAVYPKPRDNPVANNAISGKEDHHSSSGNWSESSSTRHSQTSDTIPSHTVMMLSLGDSAVSLSTPGNVEAGSQSVNYSCRSNLASPNQSQDSDGRSESSYSGERTQGVVSSTEHWLYKSAENSETASCKVACTTPGCATPGSNLSSSSLERTSAKEDSTSLYSVDHDGYYSSMSMDSGLKSDMPCYSTNGFGNPRDSVINVFEAKEKKHQDDQPGQGEKSLARNISLKKAKKPPLPPSRTDSLRRVPKKKAQSNGQVLDETLIATLQHSLQLNLKCKNGSSPSQSPCSDYEDPWVLRSRSQSSVSASSSMMSTTAPNLYSICTVTPSQSETSSIKSEYADQWGYYSDYAAVADDQMKSPVTHSASTSSALSDYSISHFSDGSRASVPQVPAGLAKPKSTSPEKSHRVTSPSSGYSSQSNTPTALTPVPVFLKSASSGNGKSKLKPKVPERKSSLLSSVSISSSSTSLSSNTSTEGSVNVKKLDPTPSSPPDSGAPLPPPPLLAPPPHCLELSPPSSPPPPPAEAVDISPLPASPTFPPPPPEADVNSSFAHSVQWLPQEASIGSYSSPVPPPVFPLVVPPPAPPLDPKLTKGAMICPQYSFKKHDQEDSCYNAVKQPFSKQDASRPVMPLVTTKALQMVQLRSIRKATEGDPSPESASETISQEKGIVTSSSQSSLRPSLSLRLSNSLGEEEMKIHGTSFKNTPQTLAQSSPLVLSDNVPMLESDQKAAVTMGLSKSLEPDSPGSAPEATPKSPVRSEDFHSGMPLQGSPASPDKTQVVLPNKKPPPISKKPKLFLVVPPPQLDLTVEKIAEVSDTVRSTSSPTKRDAVLTHCEEARNCLTDGLSSSEMDSGSLVPEGGAARVTFSEAVGVGAFVVQSAASPVQEEPRQEEQSVFDEGSSSGSSQDSSSNTEGHPSQENESAEVFESETANSSFLPSNNYGEETDGVATPARPRTTEDLFAAIHRSKRKVLGRKDSEDDRTRNHSPSPPVTPTGASPTLSTLKQAGSIQRSVRKSSTSNDNFKALLLKKGSRCDTSSRMSAAEMLKHTDPRFHRAKVDASPDLSDSPASCSPSKSKRAQEEWAKSEGLMPRSMSLSSTRYGRSRTPPSAASSKYNVRNRIQSSPMTVISEGDGEVVEQTESRVQRTLDEQRERQLDVFNSDEIDMNDFSYSEELDCKETLDPTHLDLMTQPGTSRKYLSPSAEES

Target ORF information:

RefSeq Version	XM_419717.5
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NHS-like 1 (NHSL1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_419717.5

ORF Insert Sequence:

ATGGTGGTTT TCATCAACAC GAAGCTTAAA TCTCTCATGA AACTTTTCAA GAGAAAGACT 
GTTTCCAACT TAGATGAAGA GAGCAGATGG ACCGTCCATT ACACTGCTCC ATGGCACCAG 
CAGGAAAATG TGTTCCTGCC TTCCTCAAGG CCGCCTTGTG TGGAGGACTT GCACCGGCAA 
GCAAAGCTGA ACCTCAAGTC AGTACTGAGA GAGTGTGACA AGCTGCGGAG AGATGGCTAT 
CGGAGCTCTC AGTATTACTC TCAGGGCCCC ACCTTCTCCT CATCCTCCAG TGCAATCTGT 
GGAAGTTACC AGGACGATTA TGAAGAAATT GAACAAAAGT GTTCTGTCTC TTCCCCAGAA 
GAAGAAAAGC TTATCACCAT CAAAAGGCCT AAAACCCCAG TTTCAAATGA CTTATCAGAT 
ATCAACACAC AAACCAACTG GACTAAGTCA CTTCCACTGC CAACACCAGA AGAGAAGATG 
CGACAGCAAG CACAGGCAGT CCAAACAGAT GTGGTTCCTA TCAATGTGAC TGGGGAGAAT 
TTCGACCGCC AAGCCAGCAT TCGGCGGTCT CTAATTTACA CAGACACGGT GGTAAGACGG 
CCGAAGAAAG TCAAAAGGAG AAAGACTATA ACAGGAATCC CTGACAACAT ACAAAAGGAG 
CTAGCAGTTG GCACAGGCCA AAGTGATTTC CGAGGGCACT CGATGTATGT CCCAGATCAC 
AGTTCCACGC TAGGGCGACT AGACAGCTAT CGCTCTGCTA TGCAGCGGTC TGAAACCAAG 
GACACCAGCT GCCAGACTGA AGAAGTTAAA GTTGTGCCTC CTTCGATGAG AAGAATACGA 
GCACAGAAAG GACAAGGCAT TGCAGCTCAG ATGTCTCAGT TCTCCAGCTC ATCTGGAAAC 
ATGTCTGTGA TGAGTGATTC TGCTGCAGTT ATATTTGCTT CTCGCCAGAA TAGTGACATA 
GGTTTTCACA GCTTGCCTCG GGCTGGTGCA AGAGTGTCTC TGCAGTCCTT AGAACAGACC 
CAGAGCATCT CCAGACAGAC AGAAGACATT GCTGGTACTT TGCCTCACCA GATGAGTAAA 
CTGCAAGTGG ATGATGATGT TGCACATCTG AGAAATAATG CCATAACAGG AACTGTTTCG 
AGGCCAAAGT CTCAAGAGGT AAGAAGTTGT GACAGTGAAA AGTCCACTAG CCCAGCATGT 
GTGGTCTCTC CTCATGCCAC CTACTCAACA AGCGTCATAC CCAATGCGAC ACTGTCATCC 
TCCTCAGAAG TTATTGCTAT TCACACTGCC CAGAGTACTG GATCGCTGGA TAACAAAATT 
AGCAGTTCTG CTGTGTACCC AAAACCAAGA GATAATCCTG TTGCCAACAA TGCAATAAGT 
GGGAAAGAAG ATCACCATTC TTCAAGTGGT AACTGGAGTG AGAGTAGCTC CACACGTCAC 
TCACAGACTT CAGATACCAT CCCATCTCAT ACTGTCATGA TGCTTTCTCT TGGTGACTCT 
GCTGTCTCTC TCAGCACTCC TGGAAATGTA GAGGCTGGCT CTCAGAGTGT GAACTACAGC 
TGCAGAAGCA ATCTTGCTTC ACCAAACCAG TCCCAGGACA GCGATGGTAG GAGTGAATCT 
AGCTACTCAG GGGAGCGAAC ACAAGGTGTA GTGAGCAGCA CAGAGCACTG GCTATACAAG 
TCTGCGGAAA ATAGTGAGAC TGCATCATGC AAGGTAGCTT GTACCACACC AGGCTGTGCC 
ACTCCTGGTA GCAACCTGAG CAGCAGCAGC CTGGAGAGGA CATCAGCCAA AGAAGATTCC 
ACTTCACTGT ATTCTGTAGA CCACGATGGT TATTACAGCT CTATGAGCAT GGACTCTGGA 
CTGAAGTCAG ACATGCCATG CTATAGTACT AATGGTTTTG GGAACCCCAG AGACAGCGTG 
ATTAATGTCT TTGAAGCAAA GGAGAAGAAA CATCAAGATG ACCAGCCAGG CCAAGGTGAA 
AAATCCCTTG CAAGAAACAT CTCTCTAAAA AAGGCAAAGA AGCCGCCTTT ACCTCCGTCT 
AGAACAGACT CACTCAGAAG GGTACCCAAG AAGAAAGCTC AGTCCAATGG ACAGGTACTT 
GATGAAACCC TCATTGCTAC CCTCCAGCAT TCTCTGCAGT TGAATCTCAA GTGCAAAAAT 
GGCAGCTCCC CTTCCCAGAG CCCCTGCAGT GACTATGAGG ATCCCTGGGT GTTGCGCTCT 
CGCAGCCAAA GCTCGGTCAG CGCAAGCAGC AGCATGATGT CCACTACTGC CCCAAACCTG 
TACTCCATTT GCACAGTCAC TCCCTCTCAG AGTGAAACAA GCAGCATCAA GTCAGAGTAT 
GCTGACCAGT GGGGTTACTA CAGTGACTAT GCTGCAGTGG CAGATGACCA GATGAAGTCT 
CCAGTGACCC ATTCTGCTAG TACCTCTTCT GCTCTCAGTG ATTACAGCAT CAGCCACTTC 
AGTGATGGCT CAAGGGCTTC TGTACCACAA GTGCCCGCTG GACTGGCCAA ACCAAAGAGC 
ACTTCTCCAG AGAAGTCCCA CCGAGTCACA TCGCCATCCA GTGGGTACTC CAGCCAGTCC 
AATACACCCA CTGCACTCAC TCCAGTGCCT GTGTTTTTAA AATCTGCGTC ATCAGGAAAT 
GGGAAATCCA AGCTGAAACC TAAAGTGCCT GAAAGGAAAT CTTCTCTTCT GTCTTCAGTC 
TCCATTTCTT CATCCTCTAC TTCTCTTTCT TCAAATACAT CTACTGAAGG GAGTGTGAAT 
GTGAAGAAAT TGGACCCCAC CCCGAGTTCT CCTCCAGATT CTGGTGCACC TCTTCCTCCG 
CCTCCTCTTC TAGCACCTCC TCCACATTGC CTTGAGCTCT CTCCTCCCTC TTCCCCTCCT 
CCTCCTCCAG CAGAAGCCGT GGATATATCA CCATTGCCAG CTTCTCCCAC ATTCCCCCCC 
CCTCCACCAG AAGCTGATGT AAATTCTTCC TTTGCTCACA GTGTCCAGTG GTTACCACAA 
GAAGCTTCCA TCGGTTCGTA CTCTTCCCCT GTTCCTCCTC CTGTTTTCCC TTTAGTTGTC 
CCACCACCAG CCCCACCTCT TGATCCCAAA CTGACAAAAG GTGCAATGAT ATGCCCACAG 
TATTCATTTA AGAAACATGA TCAGGAAGAT TCTTGCTACA ATGCTGTGAA ACAGCCATTC 
AGCAAGCAAG ATGCATCAAG ACCTGTGATG CCCTTGGTAA CCACCAAAGC ATTGCAAATG 
GTGCAGCTGA GGTCTATCAG AAAAGCAACA GAAGGTGACC CATCACCTGA ATCTGCTTCT 
GAAACCATCT CTCAGGAAAA GGGTATTGTC ACTTCATCAT CACAGTCTTC CCTAAGGCCA 
TCTCTCTCAC TACGACTCAG TAACAGCTTA GGTGAAGAGG AGATGAAGAT TCATGGCACT 
TCATTTAAAA ACACACCTCA AACACTGGCT CAGAGTTCTC CTCTGGTACT CTCTGACAAC 
GTACCCATGC TTGAGAGCGA TCAAAAAGCT GCAGTTACTA TGGGTCTAAG CAAGTCTTTG 
GAACCAGATT CCCCAGGGTC AGCTCCAGAA GCCACACCCA AGTCCCCAGT TCGGAGCGAA 
GACTTCCATT CTGGCATGCC ACTGCAGGGG TCACCAGCAT CTCCAGACAA GACTCAGGTC 
GTTTTGCCAA ACAAGAAACC ACCTCCCATT TCAAAGAAAC CCAAACTGTT CCTTGTTGTA 
CCACCTCCAC AGTTAGATCT CACAGTGGAG AAAATAGCTG AAGTGAGTGA TACTGTCAGA 
AGCACATCAA GCCCAACTAA GAGAGACGCT GTGCTTACAC ACTGCGAGGA GGCGAGAAAT 
TGCCTTACAG ATGGACTCAG CTCCAGCGAG ATGGACTCTG GCAGCCTGGT TCCTGAGGGA 
GGAGCTGCCA GAGTCACCTT CTCTGAAGCA GTGGGAGTTG GTGCCTTTGT GGTACAGTCC 
GCTGCATCAC CAGTTCAAGA GGAGCCCAGG CAAGAGGAGC AGTCAGTTTT TGATGAAGGA 
AGCAGCTCAG GCAGCAGCCA AGACAGCAGC AGTAACACTG AAGGACACCC GTCTCAGGAG 
AACGAAAGTG CCGAGGTGTT TGAATCAGAA ACAGCAAATA GTTCATTCCT GCCAAGCAAT 
AATTATGGGG AAGAGACGGA TGGAGTGGCA ACACCAGCGA GACCGAGGAC TACTGAGGAT 
CTTTTTGCAG CCATTCACAG ATCCAAAAGG AAAGTCCTTG GCCGTAAGGA TTCTGAGGAC 
GATCGTACCC GCAACCATTC TCCTTCACCC CCCGTAACAC CCACTGGTGC TTCCCCAACC 
TTATCTACCC TCAAACAGGC AGGATCTATT CAGAGAAGCG TTCGCAAGAG CAGCACCAGC 
AATGACAACT TCAAAGCCCT GCTGCTGAAG AAAGGGAGTC GCTGTGACAC CAGTTCCCGC 
ATGTCAGCAG CAGAGATGTT GAAGCACACG GATCCACGGT TCCACCGAGC CAAGGTGGAT 
GCCTCTCCCG ACCTTTCCGA CAGCCCTGCC AGCTGCTCGC CCAGCAAGAG CAAACGGGCC 
CAAGAGGAGT GGGCCAAGAG TGAAGGCCTG ATGCCGAGGA GCATGTCCCT CTCCAGCACT 
AGGTATGGCC GATCACGAAC ACCGCCCTCC GCTGCCAGCA GCAAGTACAA TGTTCGCAAC 
CGCATCCAGA GCAGCCCCAT GACAGTGATT AGCGAAGGAG ATGGGGAAGT GGTGGAGCAG 
ACAGAGAGCA GGGTCCAAAG GACTTTGGAT GAGCAGCGAG AGAGGCAGCT CGATGTATTT 
AACAGTGATG AAATAGACAT GAATGACTTT TCGTATTCTG AGGAGCTGGA CTGCAAGGAG 
ACCTTGGATC CAACCCATCT GGACTTAATG ACTCAGCCGG GTACTTCGAG AAAGTACCTA 
AGTCCTTCAG CTGAAGAAAG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_004935693.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004935750.1 Gallus gallus NHS like 1 (NHSL1), transcript variant X11, mRNA.

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CloneID	OGa06411
Clone ID Related Accession (Same CDS sequence)	XM_004935693.3 , XM_004935693.2
Accession Version	XM_004935693.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	NHS-like protein 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004935693.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTGCC TGAAGGCTGT TTCCAACTTA GATGAAGAGA GCAGATGGAC CGTCCATTAC 
ACTGCTCCAT GGCACCAGCA GGAAAATGTG TTCCTGCCTT CCTCAAGGCC GCCTTGTGTG 
GAGGACTTGC ACCGGCAAGC AAAGCTGAAC CTCAAGTCAG TACTGAGAGA GTGTGACAAG 
CTGCGGAGAG ATGGCTATCG GAGCTCTCAG TATTACTCTC AGGGCCCCAC CTTCTCCTCA 
TCCTCCAGTG CAATCTGTGG AAGTTACCAG GACGATTATG AAGAAATTGA ACAAAAGTGT 
TCTGTCTCTT CCCCAGAAGA AGAAAAGCTT ATCACCATCA AAAGGCCTAA AACCCCAGTT 
TCAAATGACT TATCAGATAT CAACACACAA ACCAACTGGA CTAAGTCACT TCCACTGCCA 
ACACCAGAAG AGAAGATGCG ACAGCAAGCA CAGGCAGTCC AAACAGATGT GGTTCCTATC 
AATGTGACTG GGGAGAATTT CGACCGCCAA GCCAGCATTC GGCGGTCTCT AATTTACACA 
GACACGGTGG TAAGACGGCC GAAGAAAGTC AAAAGGAGAA AGACTATAAC AGGAATCCCT 
GACAACATAC AAAAGGAGCT AGCAGTTGGC ACAGGCCAAA GTGATTTCCG AGGGCACTCG 
ATGTATGTCC CAGATCACAG TTCCACGCTA GGGCGACTAG ACAGCTATCG CTCTGCTATG 
CAGCGGTCTG AAACCAAGGA CACCAGCTGC CAGACTGAAG AAGTTAAAGT TGTGCCTCCT 
TCGATGAGAA GAATACGAGC ACAGAAAGGA CAAGGCATTG CAGCTCAGAT GTCTCAGTTC 
TCCAGCTCAT CTGGAAACAT GTCTGTGATG AGTGATTCTG CTGCAGTTAT ATTTGCTTCT 
CGCCAGAATA GTGACATAGG TTTTCACAGC TTGCCTCGGG CTGGTGCAAG AGTGTCTCTG 
CAGTCCTTAG AACAGACCCA GAGCATCTCC AGACAGACAG AAGACATTGC TGGTACTTTG 
CCTCACCAGA TGAGTAAACT GCAAGTGGAT GATGATGTTG CACATCTGAG AAATAATGCC 
ATAACAGGAA CTGTTTCGAG GCCAAAGTCT CAAGAGGTAA GAAGTTGTGA CAGTGAAAAG 
TCCACTAGCC CAGCATGTGT GGTCTCTCCT CATGCCACCT ACTCAACAAG CGTCATACCC 
AATGCGACAC TGTCATCCTC CTCAGAAGTT ATTGCTATTC ACACTGCCCA GAGTACTGGA 
TCGCTGGATA ACAAAATTAG CAGTTCTGCT GTGTACCCAA AACCAAGAGA TAATCCTGTT 
GCCAACAATG CAATAAGTGG GAAAGAAGAT CACCATTCTT CAAGTGGTAA CTGGAGTGAG 
AGTAGCTCCA CACGTCACTC ACAGACTTCA GATACCATCC CATCTCATAC TGTCATGATG 
CTTTCTCTTG GTGACTCTGC TGTCTCTCTC AGCACTCCTG GAAATGTAGA GGCTGGCTCT 
CAGAGTGTGA ACTACAGCTG CAGAAGCAAT CTTGCTTCAC CAAACCAGTC CCAGGACAGC 
GATGGTAGGA GTGAATCTAG CTACTCAGGG GAGCGAACAC AAGGTGTAGT GAGCAGCACA 
GAGCACTGGC TATACAAGTC TGCGGAAAAT AGTGAGACTG CATCATGCAA GGTAGCTTGT 
ACCACACCAG GCTGTGCCAC TCCTGGTAGC AACCTGAGCA GCAGCAGCCT GGAGAGGACA 
TCAGCCAAAG AAGATTCCAC TTCACTGTAT TCTGTAGACC ACGATGGTTA TTACAGCTCT 
ATGAGCATGG ACTCTGGACT GAAGTCAGAC ATGCCATGCT ATAGTACTAA TGGTTTTGGG 
AACCCCAGAG ACAGCGTGAT TAATGTCTTT GAAGCAAAGG AGAAGAAACA TCAAGATGAC 
CAGCCAGGCC AAGGTGAAAA ATCCCTTGCA AGAAACATCT CTCTAAAAAA GGCAAAGAAG 
CCGCCTTTAC CTCCGTCTAG AACAGACTCA CTCAGAAGGG TACCCAAGAA GAAAGCTCAG 
TCCAATGGAC AGGTACTTGA TGAAACCCTC ATTGCTACCC TCCAGCATTC TCTGCAGTTG 
AATCTCAAGT GCAAAAATGG CAGCTCCCCT TCCCAGAGCC CCTGCAGTGA CTATGAGGAT 
CCCTGGGTGT TGCGCTCTCG CAGCCAAAGC TCGGTCAGCG CAAGCAGCAG CATGATGTCC 
ACTACTGCCC CAAACCTGTA CTCCATTTGC ACAGTCACTC CCTCTCAGAG TGAAACAAGC 
AGCATCAAGT CAGAGTATGC TGACCAGTGG GGTTACTACA GTGACTATGC TGCAGTGGCA 
GATGACCAGA TGAAGTCTCC AGTGACCCAT TCTGCTAGTA CCTCTTCTGC TCTCAGTGAT 
TACAGCATCA GCCACTTCAG TGATGGCTCA AGGGCTTCTG TACCACAAGT GCCCGCTGGA 
CTGGCCAAAC CAAAGAGCAC TTCTCCAGAG AAGTCCCACC GAGTCACATC GCCATCCAGT 
GGGTACTCCA GCCAGTCCAA TACACCCACT GCACTCACTC CAGTGCCTGT GTTTTTAAAA 
TCTGCGTCAT CAGGAAATGG GAAATCCAAG CTGAAACCTA AAGTGCCTGA AAGGAAATCT 
TCTCTTCTGT CTTCAGTCTC CATTTCTTCA TCCTCTACTT CTCTTTCTTC AAATACATCT 
ACTGAAGGGA GTGTGAATGT GAAGAAATTG GACCCCACCC CGAGTTCTCC TCCAGATTCT 
GGTGCACCTC TTCCTCCGCC TCCTCTTCTA GCACCTCCTC CACATTGCCT TGAGCTCTCT 
CCTCCCTCTT CCCCTCCTCC TCCTCCAGCA GAAGCCGTGG ATATATCACC ATTGCCAGCT 
TCTCCCACAT TCCCCCCCCC TCCACCAGAA GCTGATGTAA ATTCTTCCTT TGCTCACAGT 
GTCCAGTGGT TACCACAAGA AGCTTCCATC GGTTCGTACT CTTCCCCTGT TCCTCCTCCT 
GTTTTCCCTT TAGTTGTCCC ACCACCAGCC CCACCTCTTG ATCCCAAACT GACAAAAGGT 
GCAATGATAT GCCCACAGTA TTCATTTAAG AAACATGATC AGGAAGATTC TTGCTACAAT 
GCTGTGAAAC AGCCATTCAG CAAGCAAGAT GCATCAAGAC CTGTGATGCC CTTGGTAACC 
ACCAAAGCAT TGCAAATGGT GCAGCTGAGG TCTATCAGAA AAGCAACAGA AGGTGACCCA 
TCACCTGAAT CTGCTTCTGA AACCATCTCT CAGGAAAAGG GTATTGTCAC TTCATCATCA 
CAGTCTTCCC TAAGGCCATC TCTCTCACTA CGACTCAGTA ACAGCTTAGG TGAAGAGGAG 
ATGAAGATTC ATGGCACTTC ATTTAAAAAC ACACCTCAAA CACTGGCTCA GAGTTCTCCT 
CTGGTACTCT CTGACAACGT ACCCATGCTT GAGAGCGATC AAAAAGCTGC AGTTACTATG 
GGTCTAAGCA AGTCTTTGGA ACCAGATTCC CCAGGGTCAG CTCCAGAAGC CACACCCAAG 
TCCCCAGTTC GGAGCGAAGA CTTCCATTCT GGCATGCCAC TGCAGGGGTC ACCAGCATCT 
CCAGACAAGA CTCAGGTCGT TTTGCCAAAC AAGAAACCAC CTCCCATTTC AAAGAAACCC 
AAACTGTTCC TTGTTGTACC ACCTCCACAG TTAGATCTCA CAGTGGAGAA AATAGCTGAA 
GTGAGTGATA CTGTCAGAAG CACATCAAGC CCAACTAAGA GAGACGCTGT GCTTACACAC 
TGCGAGGAGG CGAGAAATTG CCTTACAGAT GGACTCAGCT CCAGCGAGAT GGACTCTGGC 
AGCCTGGTTC CTGAGGGAGG AGCTGCCAGA GTCACCTTCT CTGAAGCAGT GGGAGTTGGT 
GCCTTTGTGG TACAGTCCGC TGCATCACCA GTTCAAGAGG AGCCCAGGCA AGAGGAGCAG 
TCAGTTTTTG ATGAAGGAAG CAGCTCAGGC AGCAGCCAAG ACAGCAGCAG TAACACTGAA 
GGACACCCGT CTCAGGAGAA CGAAAGTGCC GAGGTGTTTG AATCAGAAAC AGCAAATAGT 
TCATTCCTGC CAAGCAATAA TTATGGGGAA GAGACGGATG GAGTGGCAAC ACCAGCGAGA 
CCGAGGACTA CTGAGGATCT TTTTGCAGCC ATTCACAGAT CCAAAAGGAA AGTCCTTGGC 
CGTAAGGATT CTGAGGACGA TCGTACCCGC AACCATTCTC CTTCACCCCC CGTAACACCC 
ACTGGTGCTT CCCCAACCTT ATCTACCCTC AAACAGGCAG GATCTATTCA GAGAAGCGTT 
CGCAAGAGCA GCACCAGCAA TGACAACTTC AAAGCCCTGC TGCTGAAGAA AGGGAGTCGC 
TGTGACACCA GTTCCCGCAT GTCAGCAGCA GAGATGTTGA AGCACACGGA TCCACGGTTC 
CACCGAGCCA AGGTGGATGC CTCTCCCGAC CTTTCCGACA GCCCTGCCAG CTGCTCGCCC 
AGCAAGAGCA AACGGGCCCA AGAGGAGTGG GCCAAGAGTG AAGGCCTGAT GCCGAGGAGC 
ATGTCCCTCT CCAGCACTAG GTATGGCCGA TCACGAACAC CGCCCTCCGC TGCCAGCAGC 
AAGTACAATG TTCGCAACCG CATCCAGAGC AGCCCCATGA CAGTGATTAG CGAAGGAGAT 
GGGGAAGTGG TGGAGCAGAC AGAGAGCAGG GTCCAAAGGA CTTTGGATGA GCAGCGAGAG 
AGGCAGCTCG ATGTATTTAA CAGTGATGAA ATAGACATGA ATGACTTTTC GTATTCTGAG 
GAGCTGGACT GCAAGGAGAC CTTGGATCCA ACCCATCTGG ACTTAATGAC TCAGCCGGGT 
ACTTCGAGAA AGTACCTAAG TCCTTCAGCT GAAGAAAGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004935750.1
CDS	31..4932
Translation	MFCLKAVSNLDEESRWTVHYTAPWHQQENVFLPSSRPPCVEDLHRQAKLNLKSVLRECDKLRRDGYRSSQYYSQGPTFSSSSSAICGSYQDDYEEIEQKCSVSSPEEEKLITIKRPKTPVSNDLSDINTQTNWTKSLPLPTPEEKMRQQAQAVQTDVVPINVTGENFDRQASIRRSLIYTDTVVRRPKKVKRRKTITGIPDNIQKELAVGTGQSDFRGHSMYVPDHSSTLGRLDSYRSAMQRSETKDTSCQTEEVKVVPPSMRRIRAQKGQGIAAQMSQFSSSSGNMSVMSDSAAVIFASRQNSDIGFHSLPRAGARVSLQSLEQTQSISRQTEDIAGTLPHQMSKLQVDDDVAHLRNNAITGTVSRPKSQEVRSCDSEKSTSPACVVSPHATYSTSVIPNATLSSSSEVIAIHTAQSTGSLDNKISSSAVYPKPRDNPVANNAISGKEDHHSSSGNWSESSSTRHSQTSDTIPSHTVMMLSLGDSAVSLSTPGNVEAGSQSVNYSCRSNLASPNQSQDSDGRSESSYSGERTQGVVSSTEHWLYKSAENSETASCKVACTTPGCATPGSNLSSSSLERTSAKEDSTSLYSVDHDGYYSSMSMDSGLKSDMPCYSTNGFGNPRDSVINVFEAKEKKHQDDQPGQGEKSLARNISLKKAKKPPLPPSRTDSLRRVPKKKAQSNGQVLDETLIATLQHSLQLNLKCKNGSSPSQSPCSDYEDPWVLRSRSQSSVSASSSMMSTTAPNLYSICTVTPSQSETSSIKSEYADQWGYYSDYAAVADDQMKSPVTHSASTSSALSDYSISHFSDGSRASVPQVPAGLAKPKSTSPEKSHRVTSPSSGYSSQSNTPTALTPVPVFLKSASSGNGKSKLKPKVPERKSSLLSSVSISSSSTSLSSNTSTEGSVNVKKLDPTPSSPPDSGAPLPPPPLLAPPPHCLELSPPSSPPPPPAEAVDISPLPASPTFPPPPPEADVNSSFAHSVQWLPQEASIGSYSSPVPPPVFPLVVPPPAPPLDPKLTKGAMICPQYSFKKHDQEDSCYNAVKQPFSKQDASRPVMPLVTTKALQMVQLRSIRKATEGDPSPESASETISQEKGIVTSSSQSSLRPSLSLRLSNSLGEEEMKIHGTSFKNTPQTLAQSSPLVLSDNVPMLESDQKAAVTMGLSKSLEPDSPGSAPEATPKSPVRSEDFHSGMPLQGSPASPDKTQVVLPNKKPPPISKKPKLFLVVPPPQLDLTVEKIAEVSDTVRSTSSPTKRDAVLTHCEEARNCLTDGLSSSEMDSGSLVPEGGAARVTFSEAVGVGAFVVQSAASPVQEEPRQEEQSVFDEGSSSGSSQDSSSNTEGHPSQENESAEVFESETANSSFLPSNNYGEETDGVATPARPRTTEDLFAAIHRSKRKVLGRKDSEDDRTRNHSPSPPVTPTGASPTLSTLKQAGSIQRSVRKSSTSNDNFKALLLKKGSRCDTSSRMSAAEMLKHTDPRFHRAKVDASPDLSDSPASCSPSKSKRAQEEWAKSEGLMPRSMSLSSTRYGRSRTPPSAASSKYNVRNRIQSSPMTVISEGDGEVVEQTESRVQRTLDEQRERQLDVFNSDEIDMNDFSYSEELDCKETLDPTHLDLMTQPGTSRKYLSPSAEES

Target ORF information:

RefSeq Version	XM_004935693.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NHS like 1 (NHSL1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004935693.3

ORF Insert Sequence:

ATGTTCTGCC TGAAGGCTGT TTCCAACTTA GATGAAGAGA GCAGATGGAC CGTCCATTAC 
ACTGCTCCAT GGCACCAGCA GGAAAATGTG TTCCTGCCTT CCTCAAGGCC GCCTTGTGTG 
GAGGACTTGC ACCGGCAAGC AAAGCTGAAC CTCAAGTCAG TACTGAGAGA GTGTGACAAG 
CTGCGGAGAG ATGGCTATCG GAGCTCTCAG TATTACTCTC AGGGCCCCAC CTTCTCCTCA 
TCCTCCAGTG CAATCTGTGG AAGTTACCAG GACGATTATG AAGAAATTGA ACAAAAGTGT 
TCTGTCTCTT CCCCAGAAGA AGAAAAGCTT ATCACCATCA AAAGGCCTAA AACCCCAGTT 
TCAAATGACT TATCAGATAT CAACACACAA ACCAACTGGA CTAAGTCACT TCCACTGCCA 
ACACCAGAAG AGAAGATGCG ACAGCAAGCA CAGGCAGTCC AAACAGATGT GGTTCCTATC 
AATGTGACTG GGGAGAATTT CGACCGCCAA GCCAGCATTC GGCGGTCTCT AATTTACACA 
GACACGGTGG TAAGACGGCC GAAGAAAGTC AAAAGGAGAA AGACTATAAC AGGAATCCCT 
GACAACATAC AAAAGGAGCT AGCAGTTGGC ACAGGCCAAA GTGATTTCCG AGGGCACTCG 
ATGTATGTCC CAGATCACAG TTCCACGCTA GGGCGACTAG ACAGCTATCG CTCTGCTATG 
CAGCGGTCTG AAACCAAGGA CACCAGCTGC CAGACTGAAG AAGTTAAAGT TGTGCCTCCT 
TCGATGAGAA GAATACGAGC ACAGAAAGGA CAAGGCATTG CAGCTCAGAT GTCTCAGTTC 
TCCAGCTCAT CTGGAAACAT GTCTGTGATG AGTGATTCTG CTGCAGTTAT ATTTGCTTCT 
CGCCAGAATA GTGACATAGG TTTTCACAGC TTGCCTCGGG CTGGTGCAAG AGTGTCTCTG 
CAGTCCTTAG AACAGACCCA GAGCATCTCC AGACAGACAG AAGACATTGC TGGTACTTTG 
CCTCACCAGA TGAGTAAACT GCAAGTGGAT GATGATGTTG CACATCTGAG AAATAATGCC 
ATAACAGGAA CTGTTTCGAG GCCAAAGTCT CAAGAGGTAA GAAGTTGTGA CAGTGAAAAG 
TCCACTAGCC CAGCATGTGT GGTCTCTCCT CATGCCACCT ACTCAACAAG CGTCATACCC 
AATGCGACAC TGTCATCCTC CTCAGAAGTT ATTGCTATTC ACACTGCCCA GAGTACTGGA 
TCGCTGGATA ACAAAATTAG CAGTTCTGCT GTGTACCCAA AACCAAGAGA TAATCCTGTT 
GCCAACAATG CAATAAGTGG GAAAGAAGAT CACCATTCTT CAAGTGGTAA CTGGAGTGAG 
AGTAGCTCCA CACGTCACTC ACAGACTTCA GATACCATCC CATCTCATAC TGTCATGATG 
CTTTCTCTTG GTGACTCTGC TGTCTCTCTC AGCACTCCTG GAAATGTAGA GGCTGGCTCT 
CAGAGTGTGA ACTACAGCTG CAGAAGCAAT CTTGCTTCAC CAAACCAGTC CCAGGACAGC 
GATGGTAGGA GTGAATCTAG CTACTCAGGG GAGCGAACAC AAGGTGTAGT GAGCAGCACA 
GAGCACTGGC TATACAAGTC TGCGGAAAAT AGTGAGACTG CATCATGCAA GGTAGCTTGT 
ACCACACCAG GCTGTGCCAC TCCTGGTAGC AACCTGAGCA GCAGCAGCCT GGAGAGGACA 
TCAGCCAAAG AAGATTCCAC TTCACTGTAT TCTGTAGACC ACGATGGTTA TTACAGCTCT 
ATGAGCATGG ACTCTGGACT GAAGTCAGAC ATGCCATGCT ATAGTACTAA TGGTTTTGGG 
AACCCCAGAG ACAGCGTGAT TAATGTCTTT GAAGCAAAGG AGAAGAAACA TCAAGATGAC 
CAGCCAGGCC AAGGTGAAAA ATCCCTTGCA AGAAACATCT CTCTAAAAAA GGCAAAGAAG 
CCGCCTTTAC CTCCGTCTAG AACAGACTCA CTCAGAAGGG TACCCAAGAA GAAAGCTCAG 
TCCAATGGAC AGGTACTTGA TGAAACCCTC ATTGCTACCC TCCAGCATTC TCTGCAGTTG 
AATCTCAAGT GCAAAAATGG CAGCTCCCCT TCCCAGAGCC CCTGCAGTGA CTATGAGGAT 
CCCTGGGTGT TGCGCTCTCG CAGCCAAAGC TCGGTCAGCG CAAGCAGCAG CATGATGTCC 
ACTACTGCCC CAAACCTGTA CTCCATTTGC ACAGTCACTC CCTCTCAGAG TGAAACAAGC 
AGCATCAAGT CAGAGTATGC TGACCAGTGG GGTTACTACA GTGACTATGC TGCAGTGGCA 
GATGACCAGA TGAAGTCTCC AGTGACCCAT TCTGCTAGTA CCTCTTCTGC TCTCAGTGAT 
TACAGCATCA GCCACTTCAG TGATGGCTCA AGGGCTTCTG TACCACAAGT GCCCGCTGGA 
CTGGCCAAAC CAAAGAGCAC TTCTCCAGAG AAGTCCCACC GAGTCACATC GCCATCCAGT 
GGGTACTCCA GCCAGTCCAA TACACCCACT GCACTCACTC CAGTGCCTGT GTTTTTAAAA 
TCTGCGTCAT CAGGAAATGG GAAATCCAAG CTGAAACCTA AAGTGCCTGA AAGGAAATCT 
TCTCTTCTGT CTTCAGTCTC CATTTCTTCA TCCTCTACTT CTCTTTCTTC AAATACATCT 
ACTGAAGGGA GTGTGAATGT GAAGAAATTG GACCCCACCC CGAGTTCTCC TCCAGATTCT 
GGTGCACCTC TTCCTCCGCC TCCTCTTCTA GCACCTCCTC CACATTGCCT TGAGCTCTCT 
CCTCCCTCTT CCCCTCCTCC TCCTCCAGCA GAAGCCGTGG ATATATCACC ATTGCCAGCT 
TCTCCCACAT TCCCCCCCCC TCCACCAGAA GCTGATGTAA ATTCTTCCTT TGCTCACAGT 
GTCCAGTGGT TACCACAAGA AGCTTCCATC GGTTCGTACT CTTCCCCTGT TCCTCCTCCT 
GTTTTCCCTT TAGTTGTCCC ACCACCAGCC CCACCTCTTG ATCCCAAACT GACAAAAGGT 
GCAATGATAT GCCCACAGTA TTCATTTAAG AAACATGATC AGGAAGATTC TTGCTACAAT 
GCTGTGAAAC AGCCATTCAG CAAGCAAGAT GCATCAAGAC CTGTGATGCC CTTGGTAACC 
ACCAAAGCAT TGCAAATGGT GCAGCTGAGG TCTATCAGAA AAGCAACAGA AGGTGACCCA 
TCACCTGAAT CTGCTTCTGA AACCATCTCT CAGGAAAAGG GTATTGTCAC TTCATCATCA 
CAGTCTTCCC TAAGGCCATC TCTCTCACTA CGACTCAGTA ACAGCTTAGG TGAAGAGGAG 
ATGAAGATTC ATGGCACTTC ATTTAAAAAC ACACCTCAAA CACTGGCTCA GAGTTCTCCT 
CTGGTACTCT CTGACAACGT ACCCATGCTT GAGAGCGATC AAAAAGCTGC AGTTACTATG 
GGTCTAAGCA AGTCTTTGGA ACCAGATTCC CCAGGGTCAG CTCCAGAAGC CACACCCAAG 
TCCCCAGTTC GGAGCGAAGA CTTCCATTCT GGCATGCCAC TGCAGGGGTC ACCAGCATCT 
CCAGACAAGA CTCAGGTCGT TTTGCCAAAC AAGAAACCAC CTCCCATTTC AAAGAAACCC 
AAACTGTTCC TTGTTGTACC ACCTCCACAG TTAGATCTCA CAGTGGAGAA AATAGCTGAA 
GTGAGTGATA CTGTCAGAAG CACATCAAGC CCAACTAAGA GAGACGCTGT GCTTACACAC 
TGCGAGGAGG CGAGAAATTG CCTTACAGAT GGACTCAGCT CCAGCGAGAT GGACTCTGGC 
AGCCTGGTTC CTGAGGGAGG AGCTGCCAGA GTCACCTTCT CTGAAGCAGT GGGAGTTGGT 
GCCTTTGTGG TACAGTCCGC TGCATCACCA GTTCAAGAGG AGCCCAGGCA AGAGGAGCAG 
TCAGTTTTTG ATGAAGGAAG CAGCTCAGGC AGCAGCCAAG ACAGCAGCAG TAACACTGAA 
GGACACCCGT CTCAGGAGAA CGAAAGTGCC GAGGTGTTTG AATCAGAAAC AGCAAATAGT 
TCATTCCTGC CAAGCAATAA TTATGGGGAA GAGACGGATG GAGTGGCAAC ACCAGCGAGA 
CCGAGGACTA CTGAGGATCT TTTTGCAGCC ATTCACAGAT CCAAAAGGAA AGTCCTTGGC 
CGTAAGGATT CTGAGGACGA TCGTACCCGC AACCATTCTC CTTCACCCCC CGTAACACCC 
ACTGGTGCTT CCCCAACCTT ATCTACCCTC AAACAGGCAG GATCTATTCA GAGAAGCGTT 
CGCAAGAGCA GCACCAGCAA TGACAACTTC AAAGCCCTGC TGCTGAAGAA AGGGAGTCGC 
TGTGACACCA GTTCCCGCAT GTCAGCAGCA GAGATGTTGA AGCACACGGA TCCACGGTTC 
CACCGAGCCA AGGTGGATGC CTCTCCCGAC CTTTCCGACA GCCCTGCCAG CTGCTCGCCC 
AGCAAGAGCA AACGGGCCCA AGAGGAGTGG GCCAAGAGTG AAGGCCTGAT GCCGAGGAGC 
ATGTCCCTCT CCAGCACTAG GTATGGCCGA TCACGAACAC CGCCCTCCGC TGCCAGCAGC 
AAGTACAATG TTCGCAACCG CATCCAGAGC AGCCCCATGA CAGTGATTAG CGAAGGAGAT 
GGGGAAGTGG TGGAGCAGAC AGAGAGCAGG GTCCAAAGGA CTTTGGATGA GCAGCGAGAG 
AGGCAGCTCG ATGTATTTAA CAGTGATGAA ATAGACATGA ATGACTTTTC GTATTCTGAG 
GAGCTGGACT GCAAGGAGAC CTTGGATCCA ACCCATCTGG ACTTAATGAC TCAGCCGGGT 
ACTTCGAGAA AGTACCTAAG TCCTTCAGCT GAAGAAAGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_004935693.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004935750.1 Gallus gallus NHS-like 1 (NHSL1), transcript variant X9, mRNA.

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CloneID	OGa06411
Clone ID Related Accession (Same CDS sequence)	XM_004935693.3 , XM_004935693.2
Accession Version	XM_004935693.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	NHS-like protein 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455837.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004935693.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTGCC TGAAGGCTGT TTCCAACTTA GATGAAGAGA GCAGATGGAC CGTCCATTAC 
ACTGCTCCAT GGCACCAGCA GGAAAATGTG TTCCTGCCTT CCTCAAGGCC GCCTTGTGTG 
GAGGACTTGC ACCGGCAAGC AAAGCTGAAC CTCAAGTCAG TACTGAGAGA GTGTGACAAG 
CTGCGGAGAG ATGGCTATCG GAGCTCTCAG TATTACTCTC AGGGCCCCAC CTTCTCCTCA 
TCCTCCAGTG CAATCTGTGG AAGTTACCAG GACGATTATG AAGAAATTGA ACAAAAGTGT 
TCTGTCTCTT CCCCAGAAGA AGAAAAGCTT ATCACCATCA AAAGGCCTAA AACCCCAGTT 
TCAAATGACT TATCAGATAT CAACACACAA ACCAACTGGA CTAAGTCACT TCCACTGCCA 
ACACCAGAAG AGAAGATGCG ACAGCAAGCA CAGGCAGTCC AAACAGATGT GGTTCCTATC 
AATGTGACTG GGGAGAATTT CGACCGCCAA GCCAGCATTC GGCGGTCTCT AATTTACACA 
GACACGGTGG TAAGACGGCC GAAGAAAGTC AAAAGGAGAA AGACTATAAC AGGAATCCCT 
GACAACATAC AAAAGGAGCT AGCAGTTGGC ACAGGCCAAA GTGATTTCCG AGGGCACTCG 
ATGTATGTCC CAGATCACAG TTCCACGCTA GGGCGACTAG ACAGCTATCG CTCTGCTATG 
CAGCGGTCTG AAACCAAGGA CACCAGCTGC CAGACTGAAG AAGTTAAAGT TGTGCCTCCT 
TCGATGAGAA GAATACGAGC ACAGAAAGGA CAAGGCATTG CAGCTCAGAT GTCTCAGTTC 
TCCAGCTCAT CTGGAAACAT GTCTGTGATG AGTGATTCTG CTGCAGTTAT ATTTGCTTCT 
CGCCAGAATA GTGACATAGG TTTTCACAGC TTGCCTCGGG CTGGTGCAAG AGTGTCTCTG 
CAGTCCTTAG AACAGACCCA GAGCATCTCC AGACAGACAG AAGACATTGC TGGTACTTTG 
CCTCACCAGA TGAGTAAACT GCAAGTGGAT GATGATGTTG CACATCTGAG AAATAATGCC 
ATAACAGGAA CTGTTTCGAG GCCAAAGTCT CAAGAGGTAA GAAGTTGTGA CAGTGAAAAG 
TCCACTAGCC CAGCATGTGT GGTCTCTCCT CATGCCACCT ACTCAACAAG CGTCATACCC 
AATGCGACAC TGTCATCCTC CTCAGAAGTT ATTGCTATTC ACACTGCCCA GAGTACTGGA 
TCGCTGGATA ACAAAATTAG CAGTTCTGCT GTGTACCCAA AACCAAGAGA TAATCCTGTT 
GCCAACAATG CAATAAGTGG GAAAGAAGAT CACCATTCTT CAAGTGGTAA CTGGAGTGAG 
AGTAGCTCCA CACGTCACTC ACAGACTTCA GATACCATCC CATCTCATAC TGTCATGATG 
CTTTCTCTTG GTGACTCTGC TGTCTCTCTC AGCACTCCTG GAAATGTAGA GGCTGGCTCT 
CAGAGTGTGA ACTACAGCTG CAGAAGCAAT CTTGCTTCAC CAAACCAGTC CCAGGACAGC 
GATGGTAGGA GTGAATCTAG CTACTCAGGG GAGCGAACAC AAGGTGTAGT GAGCAGCACA 
GAGCACTGGC TATACAAGTC TGCGGAAAAT AGTGAGACTG CATCATGCAA GGTAGCTTGT 
ACCACACCAG GCTGTGCCAC TCCTGGTAGC AACCTGAGCA GCAGCAGCCT GGAGAGGACA 
TCAGCCAAAG AAGATTCCAC TTCACTGTAT TCTGTAGACC ACGATGGTTA TTACAGCTCT 
ATGAGCATGG ACTCTGGACT GAAGTCAGAC ATGCCATGCT ATAGTACTAA TGGTTTTGGG 
AACCCCAGAG ACAGCGTGAT TAATGTCTTT GAAGCAAAGG AGAAGAAACA TCAAGATGAC 
CAGCCAGGCC AAGGTGAAAA ATCCCTTGCA AGAAACATCT CTCTAAAAAA GGCAAAGAAG 
CCGCCTTTAC CTCCGTCTAG AACAGACTCA CTCAGAAGGG TACCCAAGAA GAAAGCTCAG 
TCCAATGGAC AGGTACTTGA TGAAACCCTC ATTGCTACCC TCCAGCATTC TCTGCAGTTG 
AATCTCAAGT GCAAAAATGG CAGCTCCCCT TCCCAGAGCC CCTGCAGTGA CTATGAGGAT 
CCCTGGGTGT TGCGCTCTCG CAGCCAAAGC TCGGTCAGCG CAAGCAGCAG CATGATGTCC 
ACTACTGCCC CAAACCTGTA CTCCATTTGC ACAGTCACTC CCTCTCAGAG TGAAACAAGC 
AGCATCAAGT CAGAGTATGC TGACCAGTGG GGTTACTACA GTGACTATGC TGCAGTGGCA 
GATGACCAGA TGAAGTCTCC AGTGACCCAT TCTGCTAGTA CCTCTTCTGC TCTCAGTGAT 
TACAGCATCA GCCACTTCAG TGATGGCTCA AGGGCTTCTG TACCACAAGT GCCCGCTGGA 
CTGGCCAAAC CAAAGAGCAC TTCTCCAGAG AAGTCCCACC GAGTCACATC GCCATCCAGT 
GGGTACTCCA GCCAGTCCAA TACACCCACT GCACTCACTC CAGTGCCTGT GTTTTTAAAA 
TCTGCGTCAT CAGGAAATGG GAAATCCAAG CTGAAACCTA AAGTGCCTGA AAGGAAATCT 
TCTCTTCTGT CTTCAGTCTC CATTTCTTCA TCCTCTACTT CTCTTTCTTC AAATACATCT 
ACTGAAGGGA GTGTGAATGT GAAGAAATTG GACCCCACCC CGAGTTCTCC TCCAGATTCT 
GGTGCACCTC TTCCTCCGCC TCCTCTTCTA GCACCTCCTC CACATTGCCT TGAGCTCTCT 
CCTCCCTCTT CCCCTCCTCC TCCTCCAGCA GAAGCCGTGG ATATATCACC ATTGCCAGCT 
TCTCCCACAT TCCCCCCCCC TCCACCAGAA GCTGATGTAA ATTCTTCCTT TGCTCACAGT 
GTCCAGTGGT TACCACAAGA AGCTTCCATC GGTTCGTACT CTTCCCCTGT TCCTCCTCCT 
GTTTTCCCTT TAGTTGTCCC ACCACCAGCC CCACCTCTTG ATCCCAAACT GACAAAAGGT 
GCAATGATAT GCCCACAGTA TTCATTTAAG AAACATGATC AGGAAGATTC TTGCTACAAT 
GCTGTGAAAC AGCCATTCAG CAAGCAAGAT GCATCAAGAC CTGTGATGCC CTTGGTAACC 
ACCAAAGCAT TGCAAATGGT GCAGCTGAGG TCTATCAGAA AAGCAACAGA AGGTGACCCA 
TCACCTGAAT CTGCTTCTGA AACCATCTCT CAGGAAAAGG GTATTGTCAC TTCATCATCA 
CAGTCTTCCC TAAGGCCATC TCTCTCACTA CGACTCAGTA ACAGCTTAGG TGAAGAGGAG 
ATGAAGATTC ATGGCACTTC ATTTAAAAAC ACACCTCAAA CACTGGCTCA GAGTTCTCCT 
CTGGTACTCT CTGACAACGT ACCCATGCTT GAGAGCGATC AAAAAGCTGC AGTTACTATG 
GGTCTAAGCA AGTCTTTGGA ACCAGATTCC CCAGGGTCAG CTCCAGAAGC CACACCCAAG 
TCCCCAGTTC GGAGCGAAGA CTTCCATTCT GGCATGCCAC TGCAGGGGTC ACCAGCATCT 
CCAGACAAGA CTCAGGTCGT TTTGCCAAAC AAGAAACCAC CTCCCATTTC AAAGAAACCC 
AAACTGTTCC TTGTTGTACC ACCTCCACAG TTAGATCTCA CAGTGGAGAA AATAGCTGAA 
GTGAGTGATA CTGTCAGAAG CACATCAAGC CCAACTAAGA GAGACGCTGT GCTTACACAC 
TGCGAGGAGG CGAGAAATTG CCTTACAGAT GGACTCAGCT CCAGCGAGAT GGACTCTGGC 
AGCCTGGTTC CTGAGGGAGG AGCTGCCAGA GTCACCTTCT CTGAAGCAGT GGGAGTTGGT 
GCCTTTGTGG TACAGTCCGC TGCATCACCA GTTCAAGAGG AGCCCAGGCA AGAGGAGCAG 
TCAGTTTTTG ATGAAGGAAG CAGCTCAGGC AGCAGCCAAG ACAGCAGCAG TAACACTGAA 
GGACACCCGT CTCAGGAGAA CGAAAGTGCC GAGGTGTTTG AATCAGAAAC AGCAAATAGT 
TCATTCCTGC CAAGCAATAA TTATGGGGAA GAGACGGATG GAGTGGCAAC ACCAGCGAGA 
CCGAGGACTA CTGAGGATCT TTTTGCAGCC ATTCACAGAT CCAAAAGGAA AGTCCTTGGC 
CGTAAGGATT CTGAGGACGA TCGTACCCGC AACCATTCTC CTTCACCCCC CGTAACACCC 
ACTGGTGCTT CCCCAACCTT ATCTACCCTC AAACAGGCAG GATCTATTCA GAGAAGCGTT 
CGCAAGAGCA GCACCAGCAA TGACAACTTC AAAGCCCTGC TGCTGAAGAA AGGGAGTCGC 
TGTGACACCA GTTCCCGCAT GTCAGCAGCA GAGATGTTGA AGCACACGGA TCCACGGTTC 
CACCGAGCCA AGGTGGATGC CTCTCCCGAC CTTTCCGACA GCCCTGCCAG CTGCTCGCCC 
AGCAAGAGCA AACGGGCCCA AGAGGAGTGG GCCAAGAGTG AAGGCCTGAT GCCGAGGAGC 
ATGTCCCTCT CCAGCACTAG GTATGGCCGA TCACGAACAC CGCCCTCCGC TGCCAGCAGC 
AAGTACAATG TTCGCAACCG CATCCAGAGC AGCCCCATGA CAGTGATTAG CGAAGGAGAT 
GGGGAAGTGG TGGAGCAGAC AGAGAGCAGG GTCCAAAGGA CTTTGGATGA GCAGCGAGAG 
AGGCAGCTCG ATGTATTTAA CAGTGATGAA ATAGACATGA ATGACTTTTC GTATTCTGAG 
GAGCTGGACT GCAAGGAGAC CTTGGATCCA ACCCATCTGG ACTTAATGAC TCAGCCGGGT 
ACTTCGAGAA AGTACCTAAG TCCTTCAGCT GAAGAAAGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004935750.1
CDS	16..4917
Translation	MFCLKAVSNLDEESRWTVHYTAPWHQQENVFLPSSRPPCVEDLHRQAKLNLKSVLRECDKLRRDGYRSSQYYSQGPTFSSSSSAICGSYQDDYEEIEQKCSVSSPEEEKLITIKRPKTPVSNDLSDINTQTNWTKSLPLPTPEEKMRQQAQAVQTDVVPINVTGENFDRQASIRRSLIYTDTVVRRPKKVKRRKTITGIPDNIQKELAVGTGQSDFRGHSMYVPDHSSTLGRLDSYRSAMQRSETKDTSCQTEEVKVVPPSMRRIRAQKGQGIAAQMSQFSSSSGNMSVMSDSAAVIFASRQNSDIGFHSLPRAGARVSLQSLEQTQSISRQTEDIAGTLPHQMSKLQVDDDVAHLRNNAITGTVSRPKSQEVRSCDSEKSTSPACVVSPHATYSTSVIPNATLSSSSEVIAIHTAQSTGSLDNKISSSAVYPKPRDNPVANNAISGKEDHHSSSGNWSESSSTRHSQTSDTIPSHTVMMLSLGDSAVSLSTPGNVEAGSQSVNYSCRSNLASPNQSQDSDGRSESSYSGERTQGVVSSTEHWLYKSAENSETASCKVACTTPGCATPGSNLSSSSLERTSAKEDSTSLYSVDHDGYYSSMSMDSGLKSDMPCYSTNGFGNPRDSVINVFEAKEKKHQDDQPGQGEKSLARNISLKKAKKPPLPPSRTDSLRRVPKKKAQSNGQVLDETLIATLQHSLQLNLKCKNGSSPSQSPCSDYEDPWVLRSRSQSSVSASSSMMSTTAPNLYSICTVTPSQSETSSIKSEYADQWGYYSDYAAVADDQMKSPVTHSASTSSALSDYSISHFSDGSRASVPQVPAGLAKPKSTSPEKSHRVTSPSSGYSSQSNTPTALTPVPVFLKSASSGNGKSKLKPKVPERKSSLLSSVSISSSSTSLSSNTSTEGSVNVKKLDPTPSSPPDSGAPLPPPPLLAPPPHCLELSPPSSPPPPPAEAVDISPLPASPTFPPPPPEADVNSSFAHSVQWLPQEASIGSYSSPVPPPVFPLVVPPPAPPLDPKLTKGAMICPQYSFKKHDQEDSCYNAVKQPFSKQDASRPVMPLVTTKALQMVQLRSIRKATEGDPSPESASETISQEKGIVTSSSQSSLRPSLSLRLSNSLGEEEMKIHGTSFKNTPQTLAQSSPLVLSDNVPMLESDQKAAVTMGLSKSLEPDSPGSAPEATPKSPVRSEDFHSGMPLQGSPASPDKTQVVLPNKKPPPISKKPKLFLVVPPPQLDLTVEKIAEVSDTVRSTSSPTKRDAVLTHCEEARNCLTDGLSSSEMDSGSLVPEGGAARVTFSEAVGVGAFVVQSAASPVQEEPRQEEQSVFDEGSSSGSSQDSSSNTEGHPSQENESAEVFESETANSSFLPSNNYGEETDGVATPARPRTTEDLFAAIHRSKRKVLGRKDSEDDRTRNHSPSPPVTPTGASPTLSTLKQAGSIQRSVRKSSTSNDNFKALLLKKGSRCDTSSRMSAAEMLKHTDPRFHRAKVDASPDLSDSPASCSPSKSKRAQEEWAKSEGLMPRSMSLSSTRYGRSRTPPSAASSKYNVRNRIQSSPMTVISEGDGEVVEQTESRVQRTLDEQRERQLDVFNSDEIDMNDFSYSEELDCKETLDPTHLDLMTQPGTSRKYLSPSAEES

Target ORF information:

RefSeq Version	XM_004935693.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NHS-like 1 (NHSL1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004935693.2

ORF Insert Sequence:

ATGTTCTGCC TGAAGGCTGT TTCCAACTTA GATGAAGAGA GCAGATGGAC CGTCCATTAC 
ACTGCTCCAT GGCACCAGCA GGAAAATGTG TTCCTGCCTT CCTCAAGGCC GCCTTGTGTG 
GAGGACTTGC ACCGGCAAGC AAAGCTGAAC CTCAAGTCAG TACTGAGAGA GTGTGACAAG 
CTGCGGAGAG ATGGCTATCG GAGCTCTCAG TATTACTCTC AGGGCCCCAC CTTCTCCTCA 
TCCTCCAGTG CAATCTGTGG AAGTTACCAG GACGATTATG AAGAAATTGA ACAAAAGTGT 
TCTGTCTCTT CCCCAGAAGA AGAAAAGCTT ATCACCATCA AAAGGCCTAA AACCCCAGTT 
TCAAATGACT TATCAGATAT CAACACACAA ACCAACTGGA CTAAGTCACT TCCACTGCCA 
ACACCAGAAG AGAAGATGCG ACAGCAAGCA CAGGCAGTCC AAACAGATGT GGTTCCTATC 
AATGTGACTG GGGAGAATTT CGACCGCCAA GCCAGCATTC GGCGGTCTCT AATTTACACA 
GACACGGTGG TAAGACGGCC GAAGAAAGTC AAAAGGAGAA AGACTATAAC AGGAATCCCT 
GACAACATAC AAAAGGAGCT AGCAGTTGGC ACAGGCCAAA GTGATTTCCG AGGGCACTCG 
ATGTATGTCC CAGATCACAG TTCCACGCTA GGGCGACTAG ACAGCTATCG CTCTGCTATG 
CAGCGGTCTG AAACCAAGGA CACCAGCTGC CAGACTGAAG AAGTTAAAGT TGTGCCTCCT 
TCGATGAGAA GAATACGAGC ACAGAAAGGA CAAGGCATTG CAGCTCAGAT GTCTCAGTTC 
TCCAGCTCAT CTGGAAACAT GTCTGTGATG AGTGATTCTG CTGCAGTTAT ATTTGCTTCT 
CGCCAGAATA GTGACATAGG TTTTCACAGC TTGCCTCGGG CTGGTGCAAG AGTGTCTCTG 
CAGTCCTTAG AACAGACCCA GAGCATCTCC AGACAGACAG AAGACATTGC TGGTACTTTG 
CCTCACCAGA TGAGTAAACT GCAAGTGGAT GATGATGTTG CACATCTGAG AAATAATGCC 
ATAACAGGAA CTGTTTCGAG GCCAAAGTCT CAAGAGGTAA GAAGTTGTGA CAGTGAAAAG 
TCCACTAGCC CAGCATGTGT GGTCTCTCCT CATGCCACCT ACTCAACAAG CGTCATACCC 
AATGCGACAC TGTCATCCTC CTCAGAAGTT ATTGCTATTC ACACTGCCCA GAGTACTGGA 
TCGCTGGATA ACAAAATTAG CAGTTCTGCT GTGTACCCAA AACCAAGAGA TAATCCTGTT 
GCCAACAATG CAATAAGTGG GAAAGAAGAT CACCATTCTT CAAGTGGTAA CTGGAGTGAG 
AGTAGCTCCA CACGTCACTC ACAGACTTCA GATACCATCC CATCTCATAC TGTCATGATG 
CTTTCTCTTG GTGACTCTGC TGTCTCTCTC AGCACTCCTG GAAATGTAGA GGCTGGCTCT 
CAGAGTGTGA ACTACAGCTG CAGAAGCAAT CTTGCTTCAC CAAACCAGTC CCAGGACAGC 
GATGGTAGGA GTGAATCTAG CTACTCAGGG GAGCGAACAC AAGGTGTAGT GAGCAGCACA 
GAGCACTGGC TATACAAGTC TGCGGAAAAT AGTGAGACTG CATCATGCAA GGTAGCTTGT 
ACCACACCAG GCTGTGCCAC TCCTGGTAGC AACCTGAGCA GCAGCAGCCT GGAGAGGACA 
TCAGCCAAAG AAGATTCCAC TTCACTGTAT TCTGTAGACC ACGATGGTTA TTACAGCTCT 
ATGAGCATGG ACTCTGGACT GAAGTCAGAC ATGCCATGCT ATAGTACTAA TGGTTTTGGG 
AACCCCAGAG ACAGCGTGAT TAATGTCTTT GAAGCAAAGG AGAAGAAACA TCAAGATGAC 
CAGCCAGGCC AAGGTGAAAA ATCCCTTGCA AGAAACATCT CTCTAAAAAA GGCAAAGAAG 
CCGCCTTTAC CTCCGTCTAG AACAGACTCA CTCAGAAGGG TACCCAAGAA GAAAGCTCAG 
TCCAATGGAC AGGTACTTGA TGAAACCCTC ATTGCTACCC TCCAGCATTC TCTGCAGTTG 
AATCTCAAGT GCAAAAATGG CAGCTCCCCT TCCCAGAGCC CCTGCAGTGA CTATGAGGAT 
CCCTGGGTGT TGCGCTCTCG CAGCCAAAGC TCGGTCAGCG CAAGCAGCAG CATGATGTCC 
ACTACTGCCC CAAACCTGTA CTCCATTTGC ACAGTCACTC CCTCTCAGAG TGAAACAAGC 
AGCATCAAGT CAGAGTATGC TGACCAGTGG GGTTACTACA GTGACTATGC TGCAGTGGCA 
GATGACCAGA TGAAGTCTCC AGTGACCCAT TCTGCTAGTA CCTCTTCTGC TCTCAGTGAT 
TACAGCATCA GCCACTTCAG TGATGGCTCA AGGGCTTCTG TACCACAAGT GCCCGCTGGA 
CTGGCCAAAC CAAAGAGCAC TTCTCCAGAG AAGTCCCACC GAGTCACATC GCCATCCAGT 
GGGTACTCCA GCCAGTCCAA TACACCCACT GCACTCACTC CAGTGCCTGT GTTTTTAAAA 
TCTGCGTCAT CAGGAAATGG GAAATCCAAG CTGAAACCTA AAGTGCCTGA AAGGAAATCT 
TCTCTTCTGT CTTCAGTCTC CATTTCTTCA TCCTCTACTT CTCTTTCTTC AAATACATCT 
ACTGAAGGGA GTGTGAATGT GAAGAAATTG GACCCCACCC CGAGTTCTCC TCCAGATTCT 
GGTGCACCTC TTCCTCCGCC TCCTCTTCTA GCACCTCCTC CACATTGCCT TGAGCTCTCT 
CCTCCCTCTT CCCCTCCTCC TCCTCCAGCA GAAGCCGTGG ATATATCACC ATTGCCAGCT 
TCTCCCACAT TCCCCCCCCC TCCACCAGAA GCTGATGTAA ATTCTTCCTT TGCTCACAGT 
GTCCAGTGGT TACCACAAGA AGCTTCCATC GGTTCGTACT CTTCCCCTGT TCCTCCTCCT 
GTTTTCCCTT TAGTTGTCCC ACCACCAGCC CCACCTCTTG ATCCCAAACT GACAAAAGGT 
GCAATGATAT GCCCACAGTA TTCATTTAAG AAACATGATC AGGAAGATTC TTGCTACAAT 
GCTGTGAAAC AGCCATTCAG CAAGCAAGAT GCATCAAGAC CTGTGATGCC CTTGGTAACC 
ACCAAAGCAT TGCAAATGGT GCAGCTGAGG TCTATCAGAA AAGCAACAGA AGGTGACCCA 
TCACCTGAAT CTGCTTCTGA AACCATCTCT CAGGAAAAGG GTATTGTCAC TTCATCATCA 
CAGTCTTCCC TAAGGCCATC TCTCTCACTA CGACTCAGTA ACAGCTTAGG TGAAGAGGAG 
ATGAAGATTC ATGGCACTTC ATTTAAAAAC ACACCTCAAA CACTGGCTCA GAGTTCTCCT 
CTGGTACTCT CTGACAACGT ACCCATGCTT GAGAGCGATC AAAAAGCTGC AGTTACTATG 
GGTCTAAGCA AGTCTTTGGA ACCAGATTCC CCAGGGTCAG CTCCAGAAGC CACACCCAAG 
TCCCCAGTTC GGAGCGAAGA CTTCCATTCT GGCATGCCAC TGCAGGGGTC ACCAGCATCT 
CCAGACAAGA CTCAGGTCGT TTTGCCAAAC AAGAAACCAC CTCCCATTTC AAAGAAACCC 
AAACTGTTCC TTGTTGTACC ACCTCCACAG TTAGATCTCA CAGTGGAGAA AATAGCTGAA 
GTGAGTGATA CTGTCAGAAG CACATCAAGC CCAACTAAGA GAGACGCTGT GCTTACACAC 
TGCGAGGAGG CGAGAAATTG CCTTACAGAT GGACTCAGCT CCAGCGAGAT GGACTCTGGC 
AGCCTGGTTC CTGAGGGAGG AGCTGCCAGA GTCACCTTCT CTGAAGCAGT GGGAGTTGGT 
GCCTTTGTGG TACAGTCCGC TGCATCACCA GTTCAAGAGG AGCCCAGGCA AGAGGAGCAG 
TCAGTTTTTG ATGAAGGAAG CAGCTCAGGC AGCAGCCAAG ACAGCAGCAG TAACACTGAA 
GGACACCCGT CTCAGGAGAA CGAAAGTGCC GAGGTGTTTG AATCAGAAAC AGCAAATAGT 
TCATTCCTGC CAAGCAATAA TTATGGGGAA GAGACGGATG GAGTGGCAAC ACCAGCGAGA 
CCGAGGACTA CTGAGGATCT TTTTGCAGCC ATTCACAGAT CCAAAAGGAA AGTCCTTGGC 
CGTAAGGATT CTGAGGACGA TCGTACCCGC AACCATTCTC CTTCACCCCC CGTAACACCC 
ACTGGTGCTT CCCCAACCTT ATCTACCCTC AAACAGGCAG GATCTATTCA GAGAAGCGTT 
CGCAAGAGCA GCACCAGCAA TGACAACTTC AAAGCCCTGC TGCTGAAGAA AGGGAGTCGC 
TGTGACACCA GTTCCCGCAT GTCAGCAGCA GAGATGTTGA AGCACACGGA TCCACGGTTC 
CACCGAGCCA AGGTGGATGC CTCTCCCGAC CTTTCCGACA GCCCTGCCAG CTGCTCGCCC 
AGCAAGAGCA AACGGGCCCA AGAGGAGTGG GCCAAGAGTG AAGGCCTGAT GCCGAGGAGC 
ATGTCCCTCT CCAGCACTAG GTATGGCCGA TCACGAACAC CGCCCTCCGC TGCCAGCAGC 
AAGTACAATG TTCGCAACCG CATCCAGAGC AGCCCCATGA CAGTGATTAG CGAAGGAGAT 
GGGGAAGTGG TGGAGCAGAC AGAGAGCAGG GTCCAAAGGA CTTTGGATGA GCAGCGAGAG 
AGGCAGCTCG ATGTATTTAA CAGTGATGAA ATAGACATGA ATGACTTTTC GTATTCTGAG 
GAGCTGGACT GCAAGGAGAC CTTGGATCCA ACCCATCTGG ACTTAATGAC TCAGCCGGGT 
ACTTCGAGAA AGTACCTAAG TCCTTCAGCT GAAGAAAGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_004935689.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004935746.2 Gallus gallus NHS-like 1 (NHSL1), transcript variant X1, mRNA.

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CloneID	OGa33681
Clone ID Related Accession (Same CDS sequence)	XM_004935689.2 , XM_025149170.1 , XM_025149171.1
Accession Version	XM_004935689.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	NHS-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455837.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004935689.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTTCC CCCTGCGGAC CGTGGAGCCT CTGAAGCTGT GCAGGTTGGA GGAAGGCGAC 
GGCGCGGAGC GGGGCGGCCC GGCGCCGGGG GACCACGCCG GGGCGGCCGA AGGGGGCGGC 
GGGCGGCGGC GAGGCGCGGT GCCTCAGTTC GGGGCGCTGG AACAGGTCAG CTCGTACGCG 
CTGGTGTCGC TGCTCATGCA GCTCTCCGAC CTGTCCCGCT GCGCCGGAGA CATCTTCGGC 
GGGATCCTGA GCGAGGCGGA CACGCTGTGC CACCGCAACG CCCGGCTCCA GCGCCGTCTG 
GGAGCCCTCG AAGCTCTGCT CGCCCGCCTC GACCACCGCA AAGTGAAGAT CCCTGTTTCC 
AACTTAGATG AAGAGAGCAG ATGGACCGTC CATTACACTG CTCCATGGCA CCAGCAGGAA 
AATGTGTTCC TGCCTTCCTC AAGGCCGCCT TGTGTGGAGG ACTTGCACCG GCAAGCAAAG 
CTGAACCTCA AGTCAGTACT GAGAGAGTGT GACAAGCTGC GGAGAGATGG CTATCGGAGC 
TCTCAGTATT ACTCTCAGGG CCCCACCTTC TCCTCATCCT CCAGTGCAAT CTGTGGAAGT 
TACCAGGACG ATTATGAAGA AATTGAACAA AAGTGTTCTG TCTCTTCCCC AGAAGAAGAA 
AAGCTTATCA CCATCAAAAG GCCTAAAACC CCAGTTTCAA ATGACTTATC AGATATCAAC 
ACACAAACCA ACTGGACTAA GTCACTTCCA CTGCCAACAC CAGAAGAGAA GATGCGACAG 
CAAGCACAGG CAGTCCAAAC AGATGTGGTT CCTATCAATG TGACTGGGGA GAATTTCGAC 
CGCCAAGCCA GCATTCGGCG GTCTCTAATT TACACAGACA CGGTGGTAAG ACGGCCGAAG 
AAAGTCAAAA GGAGAAAGAC TATAACAGGA ATCCCTGACA ACATACAAAA GGAGCTAGCA 
GTTGGCACAG GCCAAAGTGA TTTCCGAGGG CACTCGATGT ATGTCCCAGA TCACAGTTCC 
ACGCTAGGGC GACTAGACAG CTATCGCTCT GCTATGCAGC GGTCTGAAAC CAAGGACACC 
AGCTGCCAGA CTGAAGAAGT TAAAGTTGTG CCTCCTTCGA TGAGAAGAAT ACGAGCACAG 
AAAGGACAAG GCATTGCAGC TCAGATGTCT CAGTTCTCCA GCTCATCTGG AAACATGTCT 
GTGATGAGTG ATTCTGCTGC AGTTATATTT GCTTCTCGCC AGAATAGTGA CATAGGTTTT 
CACAGCTTGC CTCGGGCTGG TGCAAGAGTG TCTCTGCAGT CCTTAGAACA GACCCAGAGC 
ATCTCCAGAC AGACAGAAGA CATTGCTGGT ACTTTGCCTC ACCAGATGAG TAAACTGCAA 
GTGGATGATG ATGTTGCACA TCTGAGAAAT AATGCCATAA CAGGAACTGT TTCGAGGCCA 
AAGTCTCAAG AGGTAAGAAG TTGTGACAGT GAAAAGTCCA CTAGCCCAGC ATGTGTGGTC 
TCTCCTCATG CCACCTACTC AACAAGCGTC ATACCCAATG CGACACTGTC ATCCTCCTCA 
GAAGTTATTG CTATTCACAC TGCCCAGAGT ACTGGATCGC TGGATAACAA AATTAGCAGT 
TCTGCTGTGT ACCCAAAACC AAGAGATAAT CCTGTTGCCA ACAATGCAAT AAGTGGGAAA 
GAAGATCACC ATTCTTCAAG TGGTAACTGG AGTGAGAGTA GCTCCACACG TCACTCACAG 
ACTTCAGATA CCATCCCATC TCATACTGTC ATGATGCTTT CTCTTGGTGA CTCTGCTGTC 
TCTCTCAGCA CTCCTGGAAA TGTAGAGGCT GGCTCTCAGA GTGTGAACTA CAGCTGCAGA 
AGCAATCTTG CTTCACCAAA CCAGTCCCAG GACAGCGATG GTAGGAGTGA ATCTAGCTAC 
TCAGGGGAGC GAACACAAGG TGTAGTGAGC AGCACAGAGC ACTGGCTATA CAAGTCTGCG 
GAAAATAGTG AGACTGCATC ATGCAAGGTA GCTTGTACCA CACCAGGCTG TGCCACTCCT 
GGTAGCAACC TGAGCAGCAG CAGCCTGGAG AGGACATCAG CCAAAGAAGA TTCCACTTCA 
CTGTATTCTG TAGACCACGA TGGTTATTAC AGCTCTATGA GCATGGACTC TGGACTGAAG 
TCAGACATGC CATGCTATAG TACTAATGGT TTTGGGAACC CCAGAGACAG CGTGATTAAT 
GTCTTTGAAG CAAAGGAGAA GAAACATCAA GATGACCAGC CAGGCCAAGG TGAAAAATCC 
CTTGCAAGAA ACATCTCTCT AAAAAAGGCA AAGAAGCCGC CTTTACCTCC GTCTAGAACA 
GACTCACTCA GAAGGGTACC CAAGAAGAAA GCTCAGTCCA ATGGACAGGT ACTTGATGAA 
ACCCTCATTG CTACCCTCCA GCATTCTCTG CAGTTGAATC TCAAGTGCAA AAATGGCAGC 
TCCCCTTCCC AGAGCCCCTG CAGTGACTAT GAGGATCCCT GGGTGTTGCG CTCTCGCAGC 
CAAAGCTCGG TCAGCGCAAG CAGCAGCATG ATGTCCACTA CTGCCCCAAA CCTGTACTCC 
ATTTGCACAG TCACTCCCTC TCAGAGTGAA ACAAGCAGCA TCAAGTCAGA GTATGCTGAC 
CAGTGGGGTT ACTACAGTGA CTATGCTGCA GTGGCAGATG ACCAGATGAA GTCTCCAGTG 
ACCCATTCTG CTAGTACCTC TTCTGCTCTC AGTGATTACA GCATCAGCCA CTTCAGTGAT 
GGCTCAAGGG CTTCTGTACC ACAAGTGCCC GCTGGACTGG CCAAACCAAA GAGCACTTCT 
CCAGAGAAGT CCCACCGAGT CACATCGCCA TCCAGTGGGT ACTCCAGCCA GTCCAATACA 
CCCACTGCAC TCACTCCAGT GCCTGTGTTT TTAAAATCTG CGTCATCAGG AAATGGGAAA 
TCCAAGCTGA AACCTAAAGT GCCTGAAAGG AAATCTTCTC TTCTGTCTTC AGTCTCCATT 
TCTTCATCCT CTACTTCTCT TTCTTCAAAT ACATCTACTG AAGGGAGTGT GAATGTGAAG 
AAATTGGACC CCACCCCGAG TTCTCCTCCA GATTCTGGTG CACCTCTTCC TCCGCCTCCT 
CTTCTAGCAC CTCCTCCACA TTGCCTTGAG CTCTCTCCTC CCTCTTCCCC TCCTCCTCCT 
CCAGCAGAAG CCGTGGATAT ATCACCATTG CCAGCTTCTC CCACATTCCC CCCCCCTCCA 
CCAGAAGCTG ATGTAAATTC TTCCTTTGCT CACAGTGTCC AGTGGTTACC ACAAGAAGCT 
TCCATCGGTT CGTACTCTTC CCCTGTTCCT CCTCCTGTTT TCCCTTTAGT TGTCCCACCA 
CCAGCCCCAC CTCTTGATCC CAAACTGACA AAAGGTGCAA TGATATGCCC ACAGTATTCA 
TTTAAGAAAC ATGATCAGGA AGATTCTTGC TACAATGCTG TGAAACAGCC ATTCAGCAAG 
CAAGATGCAT CAAGACCTGT GATGCCCTTG GTAACCACCA AAGCATTGCA AATGGTGCAG 
CTGAGGTCTA TCAGAAAAGC AACAGAAGGT GACCCATCAC CTGAATCTGC TTCTGAAACC 
ATCTCTCAGG AAAAGGGTAT TGTCACTTCA TCATCACAGT CTTCCCTAAG GCCATCTCTC 
TCACTACGAC TCAGTAACAG CTTAGGTGAA GAGGAGATGA AGATTCATGG CACTTCATTT 
AAAAACACAC CTCAAACACT GGCTCAGAGT TCTCCTCTGG TACTCTCTGA CAACGTACCC 
ATGCTTGAGA GCGATCAAAA AGCTGCAGTT ACTATGGGTC TAAGCAAGTC TTTGGAACCA 
GATTCCCCAG GGTCAGCTCC AGAAGCCACA CCCAAGTCCC CAGTTCGGAG CGAAGACTTC 
CATTCTGGCA TGCCACTGCA GGGGTCACCA GCATCTCCAG ACAAGACTCA GGTCGTTTTG 
CCAAACAAGA AACCACCTCC CATTTCAAAG AAACCCAAAC TGTTCCTTGT TGTACCACCT 
CCACAGTTAG ATCTCACAGT GGAGAAAATA GCTGAAGTGA GTGATACTGT CAGAAGCACA 
TCAAGCCCAA CTAAGAGAGA CGCTGTGCTT ACACACTGCG AGGAGGCGAG AAATTGCCTT 
ACAGATGGAC TCAGCTCCAG CGAGATGGAC TCTGGCAGCC TGGTTCCTGA GGGAGGAGCT 
GCCAGAGTCA CCTTCTCTGA AGCAGTGGGA GTTGGTGCCT TTGTGGTACA GTCCGCTGCA 
TCACCAGTTC AAGAGGAGCC CAGGCAAGAG GAGCAGTCAG TTTTTGATGA AGGAAGCAGC 
TCAGGCAGCA GCCAAGACAG CAGCAGTAAC ACTGAAGGAC ACCCGTCTCA GGAGAACGAA 
AGTGCCGAGG TGTTTGAATC AGAAACAGCA AATAGTTCAT TCCTGCCAAG CAATAATTAT 
GGGGAAGAGA CGGATGGAGT GGCAACACCA GCGAGACCGA GGACTACTGA GGATCTTTTT 
GCAGCCATTC ACAGATCCAA AAGGAAAGTC CTTGGCCGTA AGGATTCTGA GGACGATCGT 
ACCCGCAACC ATTCTCCTTC ACCCCCCGTA ACACCCACTG GTGCTTCCCC AACCTTATCT 
ACCCTCAAAC AGGCAGGATC TATTCAGAGA AGCGTTCGCA AGAGCAGCAC CAGCAATGAC 
AACTTCAAAG CCCTGCTGCT GAAGAAAGGG AGTCGCTGTG ACACCAGTTC CCGCATGTCA 
GCAGCAGAGA TGTTGAAGCA CACGGATCCA CGGTTCCACC GAGCCAAGGT GGATGCCTCT 
CCCGACCTTT CCGACAGCCC TGCCAGCTGC TCGCCCAGCA AGAGCAAACG GGCCCAAGAG 
GAGTGGGCCA AGAGTGAAGG CCTGATGCCG AGGAGCATGT CCCTCTCCAG CACTAGGTAT 
GGCCGATCAC GAACACCGCC CTCCGCTGCC AGCAGCAAGT ACAATGTTCG CAACCGCATC 
CAGAGCAGCC CCATGACAGT GATTAGCGAA GGAGATGGGG AAGTGGTGGA GCAGACAGAG 
AGCAGGGTCC AAAGGACTTT GGATGAGCAG CGAGAGAGGC AGCTCGATGT ATTTAACAGT 
GATGAAATAG ACATGAATGA CTTTTCGTAT TCTGAGGAGC TGGACTGCAA GGAGACCTTG 
GATCCAACCC ATCTGGACTT AATGACTCAG CCGGGTACTT CGAGAAAGTA CCTAAGTCCT 
TCAGCTGAAG AAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004935746.2
CDS	21..5258
Translation	MPFPLRTVEPLKLCRLEEGDGAERGGPAPGDHAGAAEGGGGRRRGAVPQFGALEQVSSYALVSLLMQLSDLSRCAGDIFGGILSEADTLCHRNARLQRRLGALEALLARLDHRKVKIPVSNLDEESRWTVHYTAPWHQQENVFLPSSRPPCVEDLHRQAKLNLKSVLRECDKLRRDGYRSSQYYSQGPTFSSSSSAICGSYQDDYEEIEQKCSVSSPEEEKLITIKRPKTPVSNDLSDINTQTNWTKSLPLPTPEEKMRQQAQAVQTDVVPINVTGENFDRQASIRRSLIYTDTVVRRPKKVKRRKTITGIPDNIQKELAVGTGQSDFRGHSMYVPDHSSTLGRLDSYRSAMQRSETKDTSCQTEEVKVVPPSMRRIRAQKGQGIAAQMSQFSSSSGNMSVMSDSAAVIFASRQNSDIGFHSLPRAGARVSLQSLEQTQSISRQTEDIAGTLPHQMSKLQVDDDVAHLRNNAITGTVSRPKSQEVRSCDSEKSTSPACVVSPHATYSTSVIPNATLSSSSEVIAIHTAQSTGSLDNKISSSAVYPKPRDNPVANNAISGKEDHHSSSGNWSESSSTRHSQTSDTIPSHTVMMLSLGDSAVSLSTPGNVEAGSQSVNYSCRSNLASPNQSQDSDGRSESSYSGERTQGVVSSTEHWLYKSAENSETASCKVACTTPGCATPGSNLSSSSLERTSAKEDSTSLYSVDHDGYYSSMSMDSGLKSDMPCYSTNGFGNPRDSVINVFEAKEKKHQDDQPGQGEKSLARNISLKKAKKPPLPPSRTDSLRRVPKKKAQSNGQVLDETLIATLQHSLQLNLKCKNGSSPSQSPCSDYEDPWVLRSRSQSSVSASSSMMSTTAPNLYSICTVTPSQSETSSIKSEYADQWGYYSDYAAVADDQMKSPVTHSASTSSALSDYSISHFSDGSRASVPQVPAGLAKPKSTSPEKSHRVTSPSSGYSSQSNTPTALTPVPVFLKSASSGNGKSKLKPKVPERKSSLLSSVSISSSSTSLSSNTSTEGSVNVKKLDPTPSSPPDSGAPLPPPPLLAPPPHCLELSPPSSPPPPPAEAVDISPLPASPTFPPPPPEADVNSSFAHSVQWLPQEASIGSYSSPVPPPVFPLVVPPPAPPLDPKLTKGAMICPQYSFKKHDQEDSCYNAVKQPFSKQDASRPVMPLVTTKALQMVQLRSIRKATEGDPSPESASETISQEKGIVTSSSQSSLRPSLSLRLSNSLGEEEMKIHGTSFKNTPQTLAQSSPLVLSDNVPMLESDQKAAVTMGLSKSLEPDSPGSAPEATPKSPVRSEDFHSGMPLQGSPASPDKTQVVLPNKKPPPISKKPKLFLVVPPPQLDLTVEKIAEVSDTVRSTSSPTKRDAVLTHCEEARNCLTDGLSSSEMDSGSLVPEGGAARVTFSEAVGVGAFVVQSAASPVQEEPRQEEQSVFDEGSSSGSSQDSSSNTEGHPSQENESAEVFESETANSSFLPSNNYGEETDGVATPARPRTTEDLFAAIHRSKRKVLGRKDSEDDRTRNHSPSPPVTPTGASPTLSTLKQAGSIQRSVRKSSTSNDNFKALLLKKGSRCDTSSRMSAAEMLKHTDPRFHRAKVDASPDLSDSPASCSPSKSKRAQEEWAKSEGLMPRSMSLSSTRYGRSRTPPSAASSKYNVRNRIQSSPMTVISEGDGEVVEQTESRVQRTLDEQRERQLDVFNSDEIDMNDFSYSEELDCKETLDPTHLDLMTQPGTSRKYLSPSAEES

Target ORF information:

RefSeq Version	XM_004935689.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NHS-like 1 (NHSL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004935689.2

ORF Insert Sequence:

ATGCCGTTCC CCCTGCGGAC CGTGGAGCCT CTGAAGCTGT GCAGGTTGGA GGAAGGCGAC 
GGCGCGGAGC GGGGCGGCCC GGCGCCGGGG GACCACGCCG GGGCGGCCGA AGGGGGCGGC 
GGGCGGCGGC GAGGCGCGGT GCCTCAGTTC GGGGCGCTGG AACAGGTCAG CTCGTACGCG 
CTGGTGTCGC TGCTCATGCA GCTCTCCGAC CTGTCCCGCT GCGCCGGAGA CATCTTCGGC 
GGGATCCTGA GCGAGGCGGA CACGCTGTGC CACCGCAACG CCCGGCTCCA GCGCCGTCTG 
GGAGCCCTCG AAGCTCTGCT CGCCCGCCTC GACCACCGCA AAGTGAAGAT CCCTGTTTCC 
AACTTAGATG AAGAGAGCAG ATGGACCGTC CATTACACTG CTCCATGGCA CCAGCAGGAA 
AATGTGTTCC TGCCTTCCTC AAGGCCGCCT TGTGTGGAGG ACTTGCACCG GCAAGCAAAG 
CTGAACCTCA AGTCAGTACT GAGAGAGTGT GACAAGCTGC GGAGAGATGG CTATCGGAGC 
TCTCAGTATT ACTCTCAGGG CCCCACCTTC TCCTCATCCT CCAGTGCAAT CTGTGGAAGT 
TACCAGGACG ATTATGAAGA AATTGAACAA AAGTGTTCTG TCTCTTCCCC AGAAGAAGAA 
AAGCTTATCA CCATCAAAAG GCCTAAAACC CCAGTTTCAA ATGACTTATC AGATATCAAC 
ACACAAACCA ACTGGACTAA GTCACTTCCA CTGCCAACAC CAGAAGAGAA GATGCGACAG 
CAAGCACAGG CAGTCCAAAC AGATGTGGTT CCTATCAATG TGACTGGGGA GAATTTCGAC 
CGCCAAGCCA GCATTCGGCG GTCTCTAATT TACACAGACA CGGTGGTAAG ACGGCCGAAG 
AAAGTCAAAA GGAGAAAGAC TATAACAGGA ATCCCTGACA ACATACAAAA GGAGCTAGCA 
GTTGGCACAG GCCAAAGTGA TTTCCGAGGG CACTCGATGT ATGTCCCAGA TCACAGTTCC 
ACGCTAGGGC GACTAGACAG CTATCGCTCT GCTATGCAGC GGTCTGAAAC CAAGGACACC 
AGCTGCCAGA CTGAAGAAGT TAAAGTTGTG CCTCCTTCGA TGAGAAGAAT ACGAGCACAG 
AAAGGACAAG GCATTGCAGC TCAGATGTCT CAGTTCTCCA GCTCATCTGG AAACATGTCT 
GTGATGAGTG ATTCTGCTGC AGTTATATTT GCTTCTCGCC AGAATAGTGA CATAGGTTTT 
CACAGCTTGC CTCGGGCTGG TGCAAGAGTG TCTCTGCAGT CCTTAGAACA GACCCAGAGC 
ATCTCCAGAC AGACAGAAGA CATTGCTGGT ACTTTGCCTC ACCAGATGAG TAAACTGCAA 
GTGGATGATG ATGTTGCACA TCTGAGAAAT AATGCCATAA CAGGAACTGT TTCGAGGCCA 
AAGTCTCAAG AGGTAAGAAG TTGTGACAGT GAAAAGTCCA CTAGCCCAGC ATGTGTGGTC 
TCTCCTCATG CCACCTACTC AACAAGCGTC ATACCCAATG CGACACTGTC ATCCTCCTCA 
GAAGTTATTG CTATTCACAC TGCCCAGAGT ACTGGATCGC TGGATAACAA AATTAGCAGT 
TCTGCTGTGT ACCCAAAACC AAGAGATAAT CCTGTTGCCA ACAATGCAAT AAGTGGGAAA 
GAAGATCACC ATTCTTCAAG TGGTAACTGG AGTGAGAGTA GCTCCACACG TCACTCACAG 
ACTTCAGATA CCATCCCATC TCATACTGTC ATGATGCTTT CTCTTGGTGA CTCTGCTGTC 
TCTCTCAGCA CTCCTGGAAA TGTAGAGGCT GGCTCTCAGA GTGTGAACTA CAGCTGCAGA 
AGCAATCTTG CTTCACCAAA CCAGTCCCAG GACAGCGATG GTAGGAGTGA ATCTAGCTAC 
TCAGGGGAGC GAACACAAGG TGTAGTGAGC AGCACAGAGC ACTGGCTATA CAAGTCTGCG 
GAAAATAGTG AGACTGCATC ATGCAAGGTA GCTTGTACCA CACCAGGCTG TGCCACTCCT 
GGTAGCAACC TGAGCAGCAG CAGCCTGGAG AGGACATCAG CCAAAGAAGA TTCCACTTCA 
CTGTATTCTG TAGACCACGA TGGTTATTAC AGCTCTATGA GCATGGACTC TGGACTGAAG 
TCAGACATGC CATGCTATAG TACTAATGGT TTTGGGAACC CCAGAGACAG CGTGATTAAT 
GTCTTTGAAG CAAAGGAGAA GAAACATCAA GATGACCAGC CAGGCCAAGG TGAAAAATCC 
CTTGCAAGAA ACATCTCTCT AAAAAAGGCA AAGAAGCCGC CTTTACCTCC GTCTAGAACA 
GACTCACTCA GAAGGGTACC CAAGAAGAAA GCTCAGTCCA ATGGACAGGT ACTTGATGAA 
ACCCTCATTG CTACCCTCCA GCATTCTCTG CAGTTGAATC TCAAGTGCAA AAATGGCAGC 
TCCCCTTCCC AGAGCCCCTG CAGTGACTAT GAGGATCCCT GGGTGTTGCG CTCTCGCAGC 
CAAAGCTCGG TCAGCGCAAG CAGCAGCATG ATGTCCACTA CTGCCCCAAA CCTGTACTCC 
ATTTGCACAG TCACTCCCTC TCAGAGTGAA ACAAGCAGCA TCAAGTCAGA GTATGCTGAC 
CAGTGGGGTT ACTACAGTGA CTATGCTGCA GTGGCAGATG ACCAGATGAA GTCTCCAGTG 
ACCCATTCTG CTAGTACCTC TTCTGCTCTC AGTGATTACA GCATCAGCCA CTTCAGTGAT 
GGCTCAAGGG CTTCTGTACC ACAAGTGCCC GCTGGACTGG CCAAACCAAA GAGCACTTCT 
CCAGAGAAGT CCCACCGAGT CACATCGCCA TCCAGTGGGT ACTCCAGCCA GTCCAATACA 
CCCACTGCAC TCACTCCAGT GCCTGTGTTT TTAAAATCTG CGTCATCAGG AAATGGGAAA 
TCCAAGCTGA AACCTAAAGT GCCTGAAAGG AAATCTTCTC TTCTGTCTTC AGTCTCCATT 
TCTTCATCCT CTACTTCTCT TTCTTCAAAT ACATCTACTG AAGGGAGTGT GAATGTGAAG 
AAATTGGACC CCACCCCGAG TTCTCCTCCA GATTCTGGTG CACCTCTTCC TCCGCCTCCT 
CTTCTAGCAC CTCCTCCACA TTGCCTTGAG CTCTCTCCTC CCTCTTCCCC TCCTCCTCCT 
CCAGCAGAAG CCGTGGATAT ATCACCATTG CCAGCTTCTC CCACATTCCC CCCCCCTCCA 
CCAGAAGCTG ATGTAAATTC TTCCTTTGCT CACAGTGTCC AGTGGTTACC ACAAGAAGCT 
TCCATCGGTT CGTACTCTTC CCCTGTTCCT CCTCCTGTTT TCCCTTTAGT TGTCCCACCA 
CCAGCCCCAC CTCTTGATCC CAAACTGACA AAAGGTGCAA TGATATGCCC ACAGTATTCA 
TTTAAGAAAC ATGATCAGGA AGATTCTTGC TACAATGCTG TGAAACAGCC ATTCAGCAAG 
CAAGATGCAT CAAGACCTGT GATGCCCTTG GTAACCACCA AAGCATTGCA AATGGTGCAG 
CTGAGGTCTA TCAGAAAAGC AACAGAAGGT GACCCATCAC CTGAATCTGC TTCTGAAACC 
ATCTCTCAGG AAAAGGGTAT TGTCACTTCA TCATCACAGT CTTCCCTAAG GCCATCTCTC 
TCACTACGAC TCAGTAACAG CTTAGGTGAA GAGGAGATGA AGATTCATGG CACTTCATTT 
AAAAACACAC CTCAAACACT GGCTCAGAGT TCTCCTCTGG TACTCTCTGA CAACGTACCC 
ATGCTTGAGA GCGATCAAAA AGCTGCAGTT ACTATGGGTC TAAGCAAGTC TTTGGAACCA 
GATTCCCCAG GGTCAGCTCC AGAAGCCACA CCCAAGTCCC CAGTTCGGAG CGAAGACTTC 
CATTCTGGCA TGCCACTGCA GGGGTCACCA GCATCTCCAG ACAAGACTCA GGTCGTTTTG 
CCAAACAAGA AACCACCTCC CATTTCAAAG AAACCCAAAC TGTTCCTTGT TGTACCACCT 
CCACAGTTAG ATCTCACAGT GGAGAAAATA GCTGAAGTGA GTGATACTGT CAGAAGCACA 
TCAAGCCCAA CTAAGAGAGA CGCTGTGCTT ACACACTGCG AGGAGGCGAG AAATTGCCTT 
ACAGATGGAC TCAGCTCCAG CGAGATGGAC TCTGGCAGCC TGGTTCCTGA GGGAGGAGCT 
GCCAGAGTCA CCTTCTCTGA AGCAGTGGGA GTTGGTGCCT TTGTGGTACA GTCCGCTGCA 
TCACCAGTTC AAGAGGAGCC CAGGCAAGAG GAGCAGTCAG TTTTTGATGA AGGAAGCAGC 
TCAGGCAGCA GCCAAGACAG CAGCAGTAAC ACTGAAGGAC ACCCGTCTCA GGAGAACGAA 
AGTGCCGAGG TGTTTGAATC AGAAACAGCA AATAGTTCAT TCCTGCCAAG CAATAATTAT 
GGGGAAGAGA CGGATGGAGT GGCAACACCA GCGAGACCGA GGACTACTGA GGATCTTTTT 
GCAGCCATTC ACAGATCCAA AAGGAAAGTC CTTGGCCGTA AGGATTCTGA GGACGATCGT 
ACCCGCAACC ATTCTCCTTC ACCCCCCGTA ACACCCACTG GTGCTTCCCC AACCTTATCT 
ACCCTCAAAC AGGCAGGATC TATTCAGAGA AGCGTTCGCA AGAGCAGCAC CAGCAATGAC 
AACTTCAAAG CCCTGCTGCT GAAGAAAGGG AGTCGCTGTG ACACCAGTTC CCGCATGTCA 
GCAGCAGAGA TGTTGAAGCA CACGGATCCA CGGTTCCACC GAGCCAAGGT GGATGCCTCT 
CCCGACCTTT CCGACAGCCC TGCCAGCTGC TCGCCCAGCA AGAGCAAACG GGCCCAAGAG 
GAGTGGGCCA AGAGTGAAGG CCTGATGCCG AGGAGCATGT CCCTCTCCAG CACTAGGTAT 
GGCCGATCAC GAACACCGCC CTCCGCTGCC AGCAGCAAGT ACAATGTTCG CAACCGCATC 
CAGAGCAGCC CCATGACAGT GATTAGCGAA GGAGATGGGG AAGTGGTGGA GCAGACAGAG 
AGCAGGGTCC AAAGGACTTT GGATGAGCAG CGAGAGAGGC AGCTCGATGT ATTTAACAGT 
GATGAAATAG ACATGAATGA CTTTTCGTAT TCTGAGGAGC TGGACTGCAA GGAGACCTTG 
GATCCAACCC ATCTGGACTT AATGACTCAG CCGGGTACTT CGAGAAAGTA CCTAAGTCCT 
TCAGCTGAAG AAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_025149170.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025004938.1 Gallus gallus NHS like 1 (NHSL1), transcript variant X1, mRNA.

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CloneID	OGa33681
Clone ID Related Accession (Same CDS sequence)	XM_004935689.2 , XM_025149170.1 , XM_025149171.1
Accession Version	XM_025149170.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	NHS-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTTCC CCCTGCGGAC CGTGGAGCCT CTGAAGCTGT GCAGGTTGGA GGAAGGCGAC 
GGCGCGGAGC GGGGCGGCCC GGCGCCGGGG GACCACGCCG GGGCGGCCGA AGGGGGCGGC 
GGGCGGCGGC GAGGCGCGGT GCCTCAGTTC GGGGCGCTGG AACAGGTCAG CTCGTACGCG 
CTGGTGTCGC TGCTCATGCA GCTCTCCGAC CTGTCCCGCT GCGCCGGAGA CATCTTCGGC 
GGGATCCTGA GCGAGGCGGA CACGCTGTGC CACCGCAACG CCCGGCTCCA GCGCCGTCTG 
GGAGCCCTCG AAGCTCTGCT CGCCCGCCTC GACCACCGCA AAGTGAAGAT CCCTGTTTCC 
AACTTAGATG AAGAGAGCAG ATGGACCGTC CATTACACTG CTCCATGGCA CCAGCAGGAA 
AATGTGTTCC TGCCTTCCTC AAGGCCGCCT TGTGTGGAGG ACTTGCACCG GCAAGCAAAG 
CTGAACCTCA AGTCAGTACT GAGAGAGTGT GACAAGCTGC GGAGAGATGG CTATCGGAGC 
TCTCAGTATT ACTCTCAGGG CCCCACCTTC TCCTCATCCT CCAGTGCAAT CTGTGGAAGT 
TACCAGGACG ATTATGAAGA AATTGAACAA AAGTGTTCTG TCTCTTCCCC AGAAGAAGAA 
AAGCTTATCA CCATCAAAAG GCCTAAAACC CCAGTTTCAA ATGACTTATC AGATATCAAC 
ACACAAACCA ACTGGACTAA GTCACTTCCA CTGCCAACAC CAGAAGAGAA GATGCGACAG 
CAAGCACAGG CAGTCCAAAC AGATGTGGTT CCTATCAATG TGACTGGGGA GAATTTCGAC 
CGCCAAGCCA GCATTCGGCG GTCTCTAATT TACACAGACA CGGTGGTAAG ACGGCCGAAG 
AAAGTCAAAA GGAGAAAGAC TATAACAGGA ATCCCTGACA ACATACAAAA GGAGCTAGCA 
GTTGGCACAG GCCAAAGTGA TTTCCGAGGG CACTCGATGT ATGTCCCAGA TCACAGTTCC 
ACGCTAGGGC GACTAGACAG CTATCGCTCT GCTATGCAGC GGTCTGAAAC CAAGGACACC 
AGCTGCCAGA CTGAAGAAGT TAAAGTTGTG CCTCCTTCGA TGAGAAGAAT ACGAGCACAG 
AAAGGACAAG GCATTGCAGC TCAGATGTCT CAGTTCTCCA GCTCATCTGG AAACATGTCT 
GTGATGAGTG ATTCTGCTGC AGTTATATTT GCTTCTCGCC AGAATAGTGA CATAGGTTTT 
CACAGCTTGC CTCGGGCTGG TGCAAGAGTG TCTCTGCAGT CCTTAGAACA GACCCAGAGC 
ATCTCCAGAC AGACAGAAGA CATTGCTGGT ACTTTGCCTC ACCAGATGAG TAAACTGCAA 
GTGGATGATG ATGTTGCACA TCTGAGAAAT AATGCCATAA CAGGAACTGT TTCGAGGCCA 
AAGTCTCAAG AGGTAAGAAG TTGTGACAGT GAAAAGTCCA CTAGCCCAGC ATGTGTGGTC 
TCTCCTCATG CCACCTACTC AACAAGCGTC ATACCCAATG CGACACTGTC ATCCTCCTCA 
GAAGTTATTG CTATTCACAC TGCCCAGAGT ACTGGATCGC TGGATAACAA AATTAGCAGT 
TCTGCTGTGT ACCCAAAACC AAGAGATAAT CCTGTTGCCA ACAATGCAAT AAGTGGGAAA 
GAAGATCACC ATTCTTCAAG TGGTAACTGG AGTGAGAGTA GCTCCACACG TCACTCACAG 
ACTTCAGATA CCATCCCATC TCATACTGTC ATGATGCTTT CTCTTGGTGA CTCTGCTGTC 
TCTCTCAGCA CTCCTGGAAA TGTAGAGGCT GGCTCTCAGA GTGTGAACTA CAGCTGCAGA 
AGCAATCTTG CTTCACCAAA CCAGTCCCAG GACAGCGATG GTAGGAGTGA ATCTAGCTAC 
TCAGGGGAGC GAACACAAGG TGTAGTGAGC AGCACAGAGC ACTGGCTATA CAAGTCTGCG 
GAAAATAGTG AGACTGCATC ATGCAAGGTA GCTTGTACCA CACCAGGCTG TGCCACTCCT 
GGTAGCAACC TGAGCAGCAG CAGCCTGGAG AGGACATCAG CCAAAGAAGA TTCCACTTCA 
CTGTATTCTG TAGACCACGA TGGTTATTAC AGCTCTATGA GCATGGACTC TGGACTGAAG 
TCAGACATGC CATGCTATAG TACTAATGGT TTTGGGAACC CCAGAGACAG CGTGATTAAT 
GTCTTTGAAG CAAAGGAGAA GAAACATCAA GATGACCAGC CAGGCCAAGG TGAAAAATCC 
CTTGCAAGAA ACATCTCTCT AAAAAAGGCA AAGAAGCCGC CTTTACCTCC GTCTAGAACA 
GACTCACTCA GAAGGGTACC CAAGAAGAAA GCTCAGTCCA ATGGACAGGT ACTTGATGAA 
ACCCTCATTG CTACCCTCCA GCATTCTCTG CAGTTGAATC TCAAGTGCAA AAATGGCAGC 
TCCCCTTCCC AGAGCCCCTG CAGTGACTAT GAGGATCCCT GGGTGTTGCG CTCTCGCAGC 
CAAAGCTCGG TCAGCGCAAG CAGCAGCATG ATGTCCACTA CTGCCCCAAA CCTGTACTCC 
ATTTGCACAG TCACTCCCTC TCAGAGTGAA ACAAGCAGCA TCAAGTCAGA GTATGCTGAC 
CAGTGGGGTT ACTACAGTGA CTATGCTGCA GTGGCAGATG ACCAGATGAA GTCTCCAGTG 
ACCCATTCTG CTAGTACCTC TTCTGCTCTC AGTGATTACA GCATCAGCCA CTTCAGTGAT 
GGCTCAAGGG CTTCTGTACC ACAAGTGCCC GCTGGACTGG CCAAACCAAA GAGCACTTCT 
CCAGAGAAGT CCCACCGAGT CACATCGCCA TCCAGTGGGT ACTCCAGCCA GTCCAATACA 
CCCACTGCAC TCACTCCAGT GCCTGTGTTT TTAAAATCTG CGTCATCAGG AAATGGGAAA 
TCCAAGCTGA AACCTAAAGT GCCTGAAAGG AAATCTTCTC TTCTGTCTTC AGTCTCCATT 
TCTTCATCCT CTACTTCTCT TTCTTCAAAT ACATCTACTG AAGGGAGTGT GAATGTGAAG 
AAATTGGACC CCACCCCGAG TTCTCCTCCA GATTCTGGTG CACCTCTTCC TCCGCCTCCT 
CTTCTAGCAC CTCCTCCACA TTGCCTTGAG CTCTCTCCTC CCTCTTCCCC TCCTCCTCCT 
CCAGCAGAAG CCGTGGATAT ATCACCATTG CCAGCTTCTC CCACATTCCC CCCCCCTCCA 
CCAGAAGCTG ATGTAAATTC TTCCTTTGCT CACAGTGTCC AGTGGTTACC ACAAGAAGCT 
TCCATCGGTT CGTACTCTTC CCCTGTTCCT CCTCCTGTTT TCCCTTTAGT TGTCCCACCA 
CCAGCCCCAC CTCTTGATCC CAAACTGACA AAAGGTGCAA TGATATGCCC ACAGTATTCA 
TTTAAGAAAC ATGATCAGGA AGATTCTTGC TACAATGCTG TGAAACAGCC ATTCAGCAAG 
CAAGATGCAT CAAGACCTGT GATGCCCTTG GTAACCACCA AAGCATTGCA AATGGTGCAG 
CTGAGGTCTA TCAGAAAAGC AACAGAAGGT GACCCATCAC CTGAATCTGC TTCTGAAACC 
ATCTCTCAGG AAAAGGGTAT TGTCACTTCA TCATCACAGT CTTCCCTAAG GCCATCTCTC 
TCACTACGAC TCAGTAACAG CTTAGGTGAA GAGGAGATGA AGATTCATGG CACTTCATTT 
AAAAACACAC CTCAAACACT GGCTCAGAGT TCTCCTCTGG TACTCTCTGA CAACGTACCC 
ATGCTTGAGA GCGATCAAAA AGCTGCAGTT ACTATGGGTC TAAGCAAGTC TTTGGAACCA 
GATTCCCCAG GGTCAGCTCC AGAAGCCACA CCCAAGTCCC CAGTTCGGAG CGAAGACTTC 
CATTCTGGCA TGCCACTGCA GGGGTCACCA GCATCTCCAG ACAAGACTCA GGTCGTTTTG 
CCAAACAAGA AACCACCTCC CATTTCAAAG AAACCCAAAC TGTTCCTTGT TGTACCACCT 
CCACAGTTAG ATCTCACAGT GGAGAAAATA GCTGAAGTGA GTGATACTGT CAGAAGCACA 
TCAAGCCCAA CTAAGAGAGA CGCTGTGCTT ACACACTGCG AGGAGGCGAG AAATTGCCTT 
ACAGATGGAC TCAGCTCCAG CGAGATGGAC TCTGGCAGCC TGGTTCCTGA GGGAGGAGCT 
GCCAGAGTCA CCTTCTCTGA AGCAGTGGGA GTTGGTGCCT TTGTGGTACA GTCCGCTGCA 
TCACCAGTTC AAGAGGAGCC CAGGCAAGAG GAGCAGTCAG TTTTTGATGA AGGAAGCAGC 
TCAGGCAGCA GCCAAGACAG CAGCAGTAAC ACTGAAGGAC ACCCGTCTCA GGAGAACGAA 
AGTGCCGAGG TGTTTGAATC AGAAACAGCA AATAGTTCAT TCCTGCCAAG CAATAATTAT 
GGGGAAGAGA CGGATGGAGT GGCAACACCA GCGAGACCGA GGACTACTGA GGATCTTTTT 
GCAGCCATTC ACAGATCCAA AAGGAAAGTC CTTGGCCGTA AGGATTCTGA GGACGATCGT 
ACCCGCAACC ATTCTCCTTC ACCCCCCGTA ACACCCACTG GTGCTTCCCC AACCTTATCT 
ACCCTCAAAC AGGCAGGATC TATTCAGAGA AGCGTTCGCA AGAGCAGCAC CAGCAATGAC 
AACTTCAAAG CCCTGCTGCT GAAGAAAGGG AGTCGCTGTG ACACCAGTTC CCGCATGTCA 
GCAGCAGAGA TGTTGAAGCA CACGGATCCA CGGTTCCACC GAGCCAAGGT GGATGCCTCT 
CCCGACCTTT CCGACAGCCC TGCCAGCTGC TCGCCCAGCA AGAGCAAACG GGCCCAAGAG 
GAGTGGGCCA AGAGTGAAGG CCTGATGCCG AGGAGCATGT CCCTCTCCAG CACTAGGTAT 
GGCCGATCAC GAACACCGCC CTCCGCTGCC AGCAGCAAGT ACAATGTTCG CAACCGCATC 
CAGAGCAGCC CCATGACAGT GATTAGCGAA GGAGATGGGG AAGTGGTGGA GCAGACAGAG 
AGCAGGGTCC AAAGGACTTT GGATGAGCAG CGAGAGAGGC AGCTCGATGT ATTTAACAGT 
GATGAAATAG ACATGAATGA CTTTTCGTAT TCTGAGGAGC TGGACTGCAA GGAGACCTTG 
GATCCAACCC ATCTGGACTT AATGACTCAG CCGGGTACTT CGAGAAAGTA CCTAAGTCCT 
TCAGCTGAAG AAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025004938.1
CDS	131..5368
Translation	MPFPLRTVEPLKLCRLEEGDGAERGGPAPGDHAGAAEGGGGRRRGAVPQFGALEQVSSYALVSLLMQLSDLSRCAGDIFGGILSEADTLCHRNARLQRRLGALEALLARLDHRKVKIPVSNLDEESRWTVHYTAPWHQQENVFLPSSRPPCVEDLHRQAKLNLKSVLRECDKLRRDGYRSSQYYSQGPTFSSSSSAICGSYQDDYEEIEQKCSVSSPEEEKLITIKRPKTPVSNDLSDINTQTNWTKSLPLPTPEEKMRQQAQAVQTDVVPINVTGENFDRQASIRRSLIYTDTVVRRPKKVKRRKTITGIPDNIQKELAVGTGQSDFRGHSMYVPDHSSTLGRLDSYRSAMQRSETKDTSCQTEEVKVVPPSMRRIRAQKGQGIAAQMSQFSSSSGNMSVMSDSAAVIFASRQNSDIGFHSLPRAGARVSLQSLEQTQSISRQTEDIAGTLPHQMSKLQVDDDVAHLRNNAITGTVSRPKSQEVRSCDSEKSTSPACVVSPHATYSTSVIPNATLSSSSEVIAIHTAQSTGSLDNKISSSAVYPKPRDNPVANNAISGKEDHHSSSGNWSESSSTRHSQTSDTIPSHTVMMLSLGDSAVSLSTPGNVEAGSQSVNYSCRSNLASPNQSQDSDGRSESSYSGERTQGVVSSTEHWLYKSAENSETASCKVACTTPGCATPGSNLSSSSLERTSAKEDSTSLYSVDHDGYYSSMSMDSGLKSDMPCYSTNGFGNPRDSVINVFEAKEKKHQDDQPGQGEKSLARNISLKKAKKPPLPPSRTDSLRRVPKKKAQSNGQVLDETLIATLQHSLQLNLKCKNGSSPSQSPCSDYEDPWVLRSRSQSSVSASSSMMSTTAPNLYSICTVTPSQSETSSIKSEYADQWGYYSDYAAVADDQMKSPVTHSASTSSALSDYSISHFSDGSRASVPQVPAGLAKPKSTSPEKSHRVTSPSSGYSSQSNTPTALTPVPVFLKSASSGNGKSKLKPKVPERKSSLLSSVSISSSSTSLSSNTSTEGSVNVKKLDPTPSSPPDSGAPLPPPPLLAPPPHCLELSPPSSPPPPPAEAVDISPLPASPTFPPPPPEADVNSSFAHSVQWLPQEASIGSYSSPVPPPVFPLVVPPPAPPLDPKLTKGAMICPQYSFKKHDQEDSCYNAVKQPFSKQDASRPVMPLVTTKALQMVQLRSIRKATEGDPSPESASETISQEKGIVTSSSQSSLRPSLSLRLSNSLGEEEMKIHGTSFKNTPQTLAQSSPLVLSDNVPMLESDQKAAVTMGLSKSLEPDSPGSAPEATPKSPVRSEDFHSGMPLQGSPASPDKTQVVLPNKKPPPISKKPKLFLVVPPPQLDLTVEKIAEVSDTVRSTSSPTKRDAVLTHCEEARNCLTDGLSSSEMDSGSLVPEGGAARVTFSEAVGVGAFVVQSAASPVQEEPRQEEQSVFDEGSSSGSSQDSSSNTEGHPSQENESAEVFESETANSSFLPSNNYGEETDGVATPARPRTTEDLFAAIHRSKRKVLGRKDSEDDRTRNHSPSPPVTPTGASPTLSTLKQAGSIQRSVRKSSTSNDNFKALLLKKGSRCDTSSRMSAAEMLKHTDPRFHRAKVDASPDLSDSPASCSPSKSKRAQEEWAKSEGLMPRSMSLSSTRYGRSRTPPSAASSKYNVRNRIQSSPMTVISEGDGEVVEQTESRVQRTLDEQRERQLDVFNSDEIDMNDFSYSEELDCKETLDPTHLDLMTQPGTSRKYLSPSAEES

Target ORF information:

RefSeq Version	XM_025149170.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NHS like 1 (NHSL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025149170.1

ORF Insert Sequence:

ATGCCGTTCC CCCTGCGGAC CGTGGAGCCT CTGAAGCTGT GCAGGTTGGA GGAAGGCGAC 
GGCGCGGAGC GGGGCGGCCC GGCGCCGGGG GACCACGCCG GGGCGGCCGA AGGGGGCGGC 
GGGCGGCGGC GAGGCGCGGT GCCTCAGTTC GGGGCGCTGG AACAGGTCAG CTCGTACGCG 
CTGGTGTCGC TGCTCATGCA GCTCTCCGAC CTGTCCCGCT GCGCCGGAGA CATCTTCGGC 
GGGATCCTGA GCGAGGCGGA CACGCTGTGC CACCGCAACG CCCGGCTCCA GCGCCGTCTG 
GGAGCCCTCG AAGCTCTGCT CGCCCGCCTC GACCACCGCA AAGTGAAGAT CCCTGTTTCC 
AACTTAGATG AAGAGAGCAG ATGGACCGTC CATTACACTG CTCCATGGCA CCAGCAGGAA 
AATGTGTTCC TGCCTTCCTC AAGGCCGCCT TGTGTGGAGG ACTTGCACCG GCAAGCAAAG 
CTGAACCTCA AGTCAGTACT GAGAGAGTGT GACAAGCTGC GGAGAGATGG CTATCGGAGC 
TCTCAGTATT ACTCTCAGGG CCCCACCTTC TCCTCATCCT CCAGTGCAAT CTGTGGAAGT 
TACCAGGACG ATTATGAAGA AATTGAACAA AAGTGTTCTG TCTCTTCCCC AGAAGAAGAA 
AAGCTTATCA CCATCAAAAG GCCTAAAACC CCAGTTTCAA ATGACTTATC AGATATCAAC 
ACACAAACCA ACTGGACTAA GTCACTTCCA CTGCCAACAC CAGAAGAGAA GATGCGACAG 
CAAGCACAGG CAGTCCAAAC AGATGTGGTT CCTATCAATG TGACTGGGGA GAATTTCGAC 
CGCCAAGCCA GCATTCGGCG GTCTCTAATT TACACAGACA CGGTGGTAAG ACGGCCGAAG 
AAAGTCAAAA GGAGAAAGAC TATAACAGGA ATCCCTGACA ACATACAAAA GGAGCTAGCA 
GTTGGCACAG GCCAAAGTGA TTTCCGAGGG CACTCGATGT ATGTCCCAGA TCACAGTTCC 
ACGCTAGGGC GACTAGACAG CTATCGCTCT GCTATGCAGC GGTCTGAAAC CAAGGACACC 
AGCTGCCAGA CTGAAGAAGT TAAAGTTGTG CCTCCTTCGA TGAGAAGAAT ACGAGCACAG 
AAAGGACAAG GCATTGCAGC TCAGATGTCT CAGTTCTCCA GCTCATCTGG AAACATGTCT 
GTGATGAGTG ATTCTGCTGC AGTTATATTT GCTTCTCGCC AGAATAGTGA CATAGGTTTT 
CACAGCTTGC CTCGGGCTGG TGCAAGAGTG TCTCTGCAGT CCTTAGAACA GACCCAGAGC 
ATCTCCAGAC AGACAGAAGA CATTGCTGGT ACTTTGCCTC ACCAGATGAG TAAACTGCAA 
GTGGATGATG ATGTTGCACA TCTGAGAAAT AATGCCATAA CAGGAACTGT TTCGAGGCCA 
AAGTCTCAAG AGGTAAGAAG TTGTGACAGT GAAAAGTCCA CTAGCCCAGC ATGTGTGGTC 
TCTCCTCATG CCACCTACTC AACAAGCGTC ATACCCAATG CGACACTGTC ATCCTCCTCA 
GAAGTTATTG CTATTCACAC TGCCCAGAGT ACTGGATCGC TGGATAACAA AATTAGCAGT 
TCTGCTGTGT ACCCAAAACC AAGAGATAAT CCTGTTGCCA ACAATGCAAT AAGTGGGAAA 
GAAGATCACC ATTCTTCAAG TGGTAACTGG AGTGAGAGTA GCTCCACACG TCACTCACAG 
ACTTCAGATA CCATCCCATC TCATACTGTC ATGATGCTTT CTCTTGGTGA CTCTGCTGTC 
TCTCTCAGCA CTCCTGGAAA TGTAGAGGCT GGCTCTCAGA GTGTGAACTA CAGCTGCAGA 
AGCAATCTTG CTTCACCAAA CCAGTCCCAG GACAGCGATG GTAGGAGTGA ATCTAGCTAC 
TCAGGGGAGC GAACACAAGG TGTAGTGAGC AGCACAGAGC ACTGGCTATA CAAGTCTGCG 
GAAAATAGTG AGACTGCATC ATGCAAGGTA GCTTGTACCA CACCAGGCTG TGCCACTCCT 
GGTAGCAACC TGAGCAGCAG CAGCCTGGAG AGGACATCAG CCAAAGAAGA TTCCACTTCA 
CTGTATTCTG TAGACCACGA TGGTTATTAC AGCTCTATGA GCATGGACTC TGGACTGAAG 
TCAGACATGC CATGCTATAG TACTAATGGT TTTGGGAACC CCAGAGACAG CGTGATTAAT 
GTCTTTGAAG CAAAGGAGAA GAAACATCAA GATGACCAGC CAGGCCAAGG TGAAAAATCC 
CTTGCAAGAA ACATCTCTCT AAAAAAGGCA AAGAAGCCGC CTTTACCTCC GTCTAGAACA 
GACTCACTCA GAAGGGTACC CAAGAAGAAA GCTCAGTCCA ATGGACAGGT ACTTGATGAA 
ACCCTCATTG CTACCCTCCA GCATTCTCTG CAGTTGAATC TCAAGTGCAA AAATGGCAGC 
TCCCCTTCCC AGAGCCCCTG CAGTGACTAT GAGGATCCCT GGGTGTTGCG CTCTCGCAGC 
CAAAGCTCGG TCAGCGCAAG CAGCAGCATG ATGTCCACTA CTGCCCCAAA CCTGTACTCC 
ATTTGCACAG TCACTCCCTC TCAGAGTGAA ACAAGCAGCA TCAAGTCAGA GTATGCTGAC 
CAGTGGGGTT ACTACAGTGA CTATGCTGCA GTGGCAGATG ACCAGATGAA GTCTCCAGTG 
ACCCATTCTG CTAGTACCTC TTCTGCTCTC AGTGATTACA GCATCAGCCA CTTCAGTGAT 
GGCTCAAGGG CTTCTGTACC ACAAGTGCCC GCTGGACTGG CCAAACCAAA GAGCACTTCT 
CCAGAGAAGT CCCACCGAGT CACATCGCCA TCCAGTGGGT ACTCCAGCCA GTCCAATACA 
CCCACTGCAC TCACTCCAGT GCCTGTGTTT TTAAAATCTG CGTCATCAGG AAATGGGAAA 
TCCAAGCTGA AACCTAAAGT GCCTGAAAGG AAATCTTCTC TTCTGTCTTC AGTCTCCATT 
TCTTCATCCT CTACTTCTCT TTCTTCAAAT ACATCTACTG AAGGGAGTGT GAATGTGAAG 
AAATTGGACC CCACCCCGAG TTCTCCTCCA GATTCTGGTG CACCTCTTCC TCCGCCTCCT 
CTTCTAGCAC CTCCTCCACA TTGCCTTGAG CTCTCTCCTC CCTCTTCCCC TCCTCCTCCT 
CCAGCAGAAG CCGTGGATAT ATCACCATTG CCAGCTTCTC CCACATTCCC CCCCCCTCCA 
CCAGAAGCTG ATGTAAATTC TTCCTTTGCT CACAGTGTCC AGTGGTTACC ACAAGAAGCT 
TCCATCGGTT CGTACTCTTC CCCTGTTCCT CCTCCTGTTT TCCCTTTAGT TGTCCCACCA 
CCAGCCCCAC CTCTTGATCC CAAACTGACA AAAGGTGCAA TGATATGCCC ACAGTATTCA 
TTTAAGAAAC ATGATCAGGA AGATTCTTGC TACAATGCTG TGAAACAGCC ATTCAGCAAG 
CAAGATGCAT CAAGACCTGT GATGCCCTTG GTAACCACCA AAGCATTGCA AATGGTGCAG 
CTGAGGTCTA TCAGAAAAGC AACAGAAGGT GACCCATCAC CTGAATCTGC TTCTGAAACC 
ATCTCTCAGG AAAAGGGTAT TGTCACTTCA TCATCACAGT CTTCCCTAAG GCCATCTCTC 
TCACTACGAC TCAGTAACAG CTTAGGTGAA GAGGAGATGA AGATTCATGG CACTTCATTT 
AAAAACACAC CTCAAACACT GGCTCAGAGT TCTCCTCTGG TACTCTCTGA CAACGTACCC 
ATGCTTGAGA GCGATCAAAA AGCTGCAGTT ACTATGGGTC TAAGCAAGTC TTTGGAACCA 
GATTCCCCAG GGTCAGCTCC AGAAGCCACA CCCAAGTCCC CAGTTCGGAG CGAAGACTTC 
CATTCTGGCA TGCCACTGCA GGGGTCACCA GCATCTCCAG ACAAGACTCA GGTCGTTTTG 
CCAAACAAGA AACCACCTCC CATTTCAAAG AAACCCAAAC TGTTCCTTGT TGTACCACCT 
CCACAGTTAG ATCTCACAGT GGAGAAAATA GCTGAAGTGA GTGATACTGT CAGAAGCACA 
TCAAGCCCAA CTAAGAGAGA CGCTGTGCTT ACACACTGCG AGGAGGCGAG AAATTGCCTT 
ACAGATGGAC TCAGCTCCAG CGAGATGGAC TCTGGCAGCC TGGTTCCTGA GGGAGGAGCT 
GCCAGAGTCA CCTTCTCTGA AGCAGTGGGA GTTGGTGCCT TTGTGGTACA GTCCGCTGCA 
TCACCAGTTC AAGAGGAGCC CAGGCAAGAG GAGCAGTCAG TTTTTGATGA AGGAAGCAGC 
TCAGGCAGCA GCCAAGACAG CAGCAGTAAC ACTGAAGGAC ACCCGTCTCA GGAGAACGAA 
AGTGCCGAGG TGTTTGAATC AGAAACAGCA AATAGTTCAT TCCTGCCAAG CAATAATTAT 
GGGGAAGAGA CGGATGGAGT GGCAACACCA GCGAGACCGA GGACTACTGA GGATCTTTTT 
GCAGCCATTC ACAGATCCAA AAGGAAAGTC CTTGGCCGTA AGGATTCTGA GGACGATCGT 
ACCCGCAACC ATTCTCCTTC ACCCCCCGTA ACACCCACTG GTGCTTCCCC AACCTTATCT 
ACCCTCAAAC AGGCAGGATC TATTCAGAGA AGCGTTCGCA AGAGCAGCAC CAGCAATGAC 
AACTTCAAAG CCCTGCTGCT GAAGAAAGGG AGTCGCTGTG ACACCAGTTC CCGCATGTCA 
GCAGCAGAGA TGTTGAAGCA CACGGATCCA CGGTTCCACC GAGCCAAGGT GGATGCCTCT 
CCCGACCTTT CCGACAGCCC TGCCAGCTGC TCGCCCAGCA AGAGCAAACG GGCCCAAGAG 
GAGTGGGCCA AGAGTGAAGG CCTGATGCCG AGGAGCATGT CCCTCTCCAG CACTAGGTAT 
GGCCGATCAC GAACACCGCC CTCCGCTGCC AGCAGCAAGT ACAATGTTCG CAACCGCATC 
CAGAGCAGCC CCATGACAGT GATTAGCGAA GGAGATGGGG AAGTGGTGGA GCAGACAGAG 
AGCAGGGTCC AAAGGACTTT GGATGAGCAG CGAGAGAGGC AGCTCGATGT ATTTAACAGT 
GATGAAATAG ACATGAATGA CTTTTCGTAT TCTGAGGAGC TGGACTGCAA GGAGACCTTG 
GATCCAACCC ATCTGGACTT AATGACTCAG CCGGGTACTT CGAGAAAGTA CCTAAGTCCT 
TCAGCTGAAG AAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_025149171.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025004939.1 Gallus gallus NHS like 1 (NHSL1), transcript variant X2, mRNA.

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CloneID	OGa33681
Clone ID Related Accession (Same CDS sequence)	XM_004935689.2 , XM_025149170.1 , XM_025149171.1
Accession Version	XM_025149171.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	NHS-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTTCC CCCTGCGGAC CGTGGAGCCT CTGAAGCTGT GCAGGTTGGA GGAAGGCGAC 
GGCGCGGAGC GGGGCGGCCC GGCGCCGGGG GACCACGCCG GGGCGGCCGA AGGGGGCGGC 
GGGCGGCGGC GAGGCGCGGT GCCTCAGTTC GGGGCGCTGG AACAGGTCAG CTCGTACGCG 
CTGGTGTCGC TGCTCATGCA GCTCTCCGAC CTGTCCCGCT GCGCCGGAGA CATCTTCGGC 
GGGATCCTGA GCGAGGCGGA CACGCTGTGC CACCGCAACG CCCGGCTCCA GCGCCGTCTG 
GGAGCCCTCG AAGCTCTGCT CGCCCGCCTC GACCACCGCA AAGTGAAGAT CCCTGTTTCC 
AACTTAGATG AAGAGAGCAG ATGGACCGTC CATTACACTG CTCCATGGCA CCAGCAGGAA 
AATGTGTTCC TGCCTTCCTC AAGGCCGCCT TGTGTGGAGG ACTTGCACCG GCAAGCAAAG 
CTGAACCTCA AGTCAGTACT GAGAGAGTGT GACAAGCTGC GGAGAGATGG CTATCGGAGC 
TCTCAGTATT ACTCTCAGGG CCCCACCTTC TCCTCATCCT CCAGTGCAAT CTGTGGAAGT 
TACCAGGACG ATTATGAAGA AATTGAACAA AAGTGTTCTG TCTCTTCCCC AGAAGAAGAA 
AAGCTTATCA CCATCAAAAG GCCTAAAACC CCAGTTTCAA ATGACTTATC AGATATCAAC 
ACACAAACCA ACTGGACTAA GTCACTTCCA CTGCCAACAC CAGAAGAGAA GATGCGACAG 
CAAGCACAGG CAGTCCAAAC AGATGTGGTT CCTATCAATG TGACTGGGGA GAATTTCGAC 
CGCCAAGCCA GCATTCGGCG GTCTCTAATT TACACAGACA CGGTGGTAAG ACGGCCGAAG 
AAAGTCAAAA GGAGAAAGAC TATAACAGGA ATCCCTGACA ACATACAAAA GGAGCTAGCA 
GTTGGCACAG GCCAAAGTGA TTTCCGAGGG CACTCGATGT ATGTCCCAGA TCACAGTTCC 
ACGCTAGGGC GACTAGACAG CTATCGCTCT GCTATGCAGC GGTCTGAAAC CAAGGACACC 
AGCTGCCAGA CTGAAGAAGT TAAAGTTGTG CCTCCTTCGA TGAGAAGAAT ACGAGCACAG 
AAAGGACAAG GCATTGCAGC TCAGATGTCT CAGTTCTCCA GCTCATCTGG AAACATGTCT 
GTGATGAGTG ATTCTGCTGC AGTTATATTT GCTTCTCGCC AGAATAGTGA CATAGGTTTT 
CACAGCTTGC CTCGGGCTGG TGCAAGAGTG TCTCTGCAGT CCTTAGAACA GACCCAGAGC 
ATCTCCAGAC AGACAGAAGA CATTGCTGGT ACTTTGCCTC ACCAGATGAG TAAACTGCAA 
GTGGATGATG ATGTTGCACA TCTGAGAAAT AATGCCATAA CAGGAACTGT TTCGAGGCCA 
AAGTCTCAAG AGGTAAGAAG TTGTGACAGT GAAAAGTCCA CTAGCCCAGC ATGTGTGGTC 
TCTCCTCATG CCACCTACTC AACAAGCGTC ATACCCAATG CGACACTGTC ATCCTCCTCA 
GAAGTTATTG CTATTCACAC TGCCCAGAGT ACTGGATCGC TGGATAACAA AATTAGCAGT 
TCTGCTGTGT ACCCAAAACC AAGAGATAAT CCTGTTGCCA ACAATGCAAT AAGTGGGAAA 
GAAGATCACC ATTCTTCAAG TGGTAACTGG AGTGAGAGTA GCTCCACACG TCACTCACAG 
ACTTCAGATA CCATCCCATC TCATACTGTC ATGATGCTTT CTCTTGGTGA CTCTGCTGTC 
TCTCTCAGCA CTCCTGGAAA TGTAGAGGCT GGCTCTCAGA GTGTGAACTA CAGCTGCAGA 
AGCAATCTTG CTTCACCAAA CCAGTCCCAG GACAGCGATG GTAGGAGTGA ATCTAGCTAC 
TCAGGGGAGC GAACACAAGG TGTAGTGAGC AGCACAGAGC ACTGGCTATA CAAGTCTGCG 
GAAAATAGTG AGACTGCATC ATGCAAGGTA GCTTGTACCA CACCAGGCTG TGCCACTCCT 
GGTAGCAACC TGAGCAGCAG CAGCCTGGAG AGGACATCAG CCAAAGAAGA TTCCACTTCA 
CTGTATTCTG TAGACCACGA TGGTTATTAC AGCTCTATGA GCATGGACTC TGGACTGAAG 
TCAGACATGC CATGCTATAG TACTAATGGT TTTGGGAACC CCAGAGACAG CGTGATTAAT 
GTCTTTGAAG CAAAGGAGAA GAAACATCAA GATGACCAGC CAGGCCAAGG TGAAAAATCC 
CTTGCAAGAA ACATCTCTCT AAAAAAGGCA AAGAAGCCGC CTTTACCTCC GTCTAGAACA 
GACTCACTCA GAAGGGTACC CAAGAAGAAA GCTCAGTCCA ATGGACAGGT ACTTGATGAA 
ACCCTCATTG CTACCCTCCA GCATTCTCTG CAGTTGAATC TCAAGTGCAA AAATGGCAGC 
TCCCCTTCCC AGAGCCCCTG CAGTGACTAT GAGGATCCCT GGGTGTTGCG CTCTCGCAGC 
CAAAGCTCGG TCAGCGCAAG CAGCAGCATG ATGTCCACTA CTGCCCCAAA CCTGTACTCC 
ATTTGCACAG TCACTCCCTC TCAGAGTGAA ACAAGCAGCA TCAAGTCAGA GTATGCTGAC 
CAGTGGGGTT ACTACAGTGA CTATGCTGCA GTGGCAGATG ACCAGATGAA GTCTCCAGTG 
ACCCATTCTG CTAGTACCTC TTCTGCTCTC AGTGATTACA GCATCAGCCA CTTCAGTGAT 
GGCTCAAGGG CTTCTGTACC ACAAGTGCCC GCTGGACTGG CCAAACCAAA GAGCACTTCT 
CCAGAGAAGT CCCACCGAGT CACATCGCCA TCCAGTGGGT ACTCCAGCCA GTCCAATACA 
CCCACTGCAC TCACTCCAGT GCCTGTGTTT TTAAAATCTG CGTCATCAGG AAATGGGAAA 
TCCAAGCTGA AACCTAAAGT GCCTGAAAGG AAATCTTCTC TTCTGTCTTC AGTCTCCATT 
TCTTCATCCT CTACTTCTCT TTCTTCAAAT ACATCTACTG AAGGGAGTGT GAATGTGAAG 
AAATTGGACC CCACCCCGAG TTCTCCTCCA GATTCTGGTG CACCTCTTCC TCCGCCTCCT 
CTTCTAGCAC CTCCTCCACA TTGCCTTGAG CTCTCTCCTC CCTCTTCCCC TCCTCCTCCT 
CCAGCAGAAG CCGTGGATAT ATCACCATTG CCAGCTTCTC CCACATTCCC CCCCCCTCCA 
CCAGAAGCTG ATGTAAATTC TTCCTTTGCT CACAGTGTCC AGTGGTTACC ACAAGAAGCT 
TCCATCGGTT CGTACTCTTC CCCTGTTCCT CCTCCTGTTT TCCCTTTAGT TGTCCCACCA 
CCAGCCCCAC CTCTTGATCC CAAACTGACA AAAGGTGCAA TGATATGCCC ACAGTATTCA 
TTTAAGAAAC ATGATCAGGA AGATTCTTGC TACAATGCTG TGAAACAGCC ATTCAGCAAG 
CAAGATGCAT CAAGACCTGT GATGCCCTTG GTAACCACCA AAGCATTGCA AATGGTGCAG 
CTGAGGTCTA TCAGAAAAGC AACAGAAGGT GACCCATCAC CTGAATCTGC TTCTGAAACC 
ATCTCTCAGG AAAAGGGTAT TGTCACTTCA TCATCACAGT CTTCCCTAAG GCCATCTCTC 
TCACTACGAC TCAGTAACAG CTTAGGTGAA GAGGAGATGA AGATTCATGG CACTTCATTT 
AAAAACACAC CTCAAACACT GGCTCAGAGT TCTCCTCTGG TACTCTCTGA CAACGTACCC 
ATGCTTGAGA GCGATCAAAA AGCTGCAGTT ACTATGGGTC TAAGCAAGTC TTTGGAACCA 
GATTCCCCAG GGTCAGCTCC AGAAGCCACA CCCAAGTCCC CAGTTCGGAG CGAAGACTTC 
CATTCTGGCA TGCCACTGCA GGGGTCACCA GCATCTCCAG ACAAGACTCA GGTCGTTTTG 
CCAAACAAGA AACCACCTCC CATTTCAAAG AAACCCAAAC TGTTCCTTGT TGTACCACCT 
CCACAGTTAG ATCTCACAGT GGAGAAAATA GCTGAAGTGA GTGATACTGT CAGAAGCACA 
TCAAGCCCAA CTAAGAGAGA CGCTGTGCTT ACACACTGCG AGGAGGCGAG AAATTGCCTT 
ACAGATGGAC TCAGCTCCAG CGAGATGGAC TCTGGCAGCC TGGTTCCTGA GGGAGGAGCT 
GCCAGAGTCA CCTTCTCTGA AGCAGTGGGA GTTGGTGCCT TTGTGGTACA GTCCGCTGCA 
TCACCAGTTC AAGAGGAGCC CAGGCAAGAG GAGCAGTCAG TTTTTGATGA AGGAAGCAGC 
TCAGGCAGCA GCCAAGACAG CAGCAGTAAC ACTGAAGGAC ACCCGTCTCA GGAGAACGAA 
AGTGCCGAGG TGTTTGAATC AGAAACAGCA AATAGTTCAT TCCTGCCAAG CAATAATTAT 
GGGGAAGAGA CGGATGGAGT GGCAACACCA GCGAGACCGA GGACTACTGA GGATCTTTTT 
GCAGCCATTC ACAGATCCAA AAGGAAAGTC CTTGGCCGTA AGGATTCTGA GGACGATCGT 
ACCCGCAACC ATTCTCCTTC ACCCCCCGTA ACACCCACTG GTGCTTCCCC AACCTTATCT 
ACCCTCAAAC AGGCAGGATC TATTCAGAGA AGCGTTCGCA AGAGCAGCAC CAGCAATGAC 
AACTTCAAAG CCCTGCTGCT GAAGAAAGGG AGTCGCTGTG ACACCAGTTC CCGCATGTCA 
GCAGCAGAGA TGTTGAAGCA CACGGATCCA CGGTTCCACC GAGCCAAGGT GGATGCCTCT 
CCCGACCTTT CCGACAGCCC TGCCAGCTGC TCGCCCAGCA AGAGCAAACG GGCCCAAGAG 
GAGTGGGCCA AGAGTGAAGG CCTGATGCCG AGGAGCATGT CCCTCTCCAG CACTAGGTAT 
GGCCGATCAC GAACACCGCC CTCCGCTGCC AGCAGCAAGT ACAATGTTCG CAACCGCATC 
CAGAGCAGCC CCATGACAGT GATTAGCGAA GGAGATGGGG AAGTGGTGGA GCAGACAGAG 
AGCAGGGTCC AAAGGACTTT GGATGAGCAG CGAGAGAGGC AGCTCGATGT ATTTAACAGT 
GATGAAATAG ACATGAATGA CTTTTCGTAT TCTGAGGAGC TGGACTGCAA GGAGACCTTG 
GATCCAACCC ATCTGGACTT AATGACTCAG CCGGGTACTT CGAGAAAGTA CCTAAGTCCT 
TCAGCTGAAG AAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025004939.1
CDS	140..5377
Translation	MPFPLRTVEPLKLCRLEEGDGAERGGPAPGDHAGAAEGGGGRRRGAVPQFGALEQVSSYALVSLLMQLSDLSRCAGDIFGGILSEADTLCHRNARLQRRLGALEALLARLDHRKVKIPVSNLDEESRWTVHYTAPWHQQENVFLPSSRPPCVEDLHRQAKLNLKSVLRECDKLRRDGYRSSQYYSQGPTFSSSSSAICGSYQDDYEEIEQKCSVSSPEEEKLITIKRPKTPVSNDLSDINTQTNWTKSLPLPTPEEKMRQQAQAVQTDVVPINVTGENFDRQASIRRSLIYTDTVVRRPKKVKRRKTITGIPDNIQKELAVGTGQSDFRGHSMYVPDHSSTLGRLDSYRSAMQRSETKDTSCQTEEVKVVPPSMRRIRAQKGQGIAAQMSQFSSSSGNMSVMSDSAAVIFASRQNSDIGFHSLPRAGARVSLQSLEQTQSISRQTEDIAGTLPHQMSKLQVDDDVAHLRNNAITGTVSRPKSQEVRSCDSEKSTSPACVVSPHATYSTSVIPNATLSSSSEVIAIHTAQSTGSLDNKISSSAVYPKPRDNPVANNAISGKEDHHSSSGNWSESSSTRHSQTSDTIPSHTVMMLSLGDSAVSLSTPGNVEAGSQSVNYSCRSNLASPNQSQDSDGRSESSYSGERTQGVVSSTEHWLYKSAENSETASCKVACTTPGCATPGSNLSSSSLERTSAKEDSTSLYSVDHDGYYSSMSMDSGLKSDMPCYSTNGFGNPRDSVINVFEAKEKKHQDDQPGQGEKSLARNISLKKAKKPPLPPSRTDSLRRVPKKKAQSNGQVLDETLIATLQHSLQLNLKCKNGSSPSQSPCSDYEDPWVLRSRSQSSVSASSSMMSTTAPNLYSICTVTPSQSETSSIKSEYADQWGYYSDYAAVADDQMKSPVTHSASTSSALSDYSISHFSDGSRASVPQVPAGLAKPKSTSPEKSHRVTSPSSGYSSQSNTPTALTPVPVFLKSASSGNGKSKLKPKVPERKSSLLSSVSISSSSTSLSSNTSTEGSVNVKKLDPTPSSPPDSGAPLPPPPLLAPPPHCLELSPPSSPPPPPAEAVDISPLPASPTFPPPPPEADVNSSFAHSVQWLPQEASIGSYSSPVPPPVFPLVVPPPAPPLDPKLTKGAMICPQYSFKKHDQEDSCYNAVKQPFSKQDASRPVMPLVTTKALQMVQLRSIRKATEGDPSPESASETISQEKGIVTSSSQSSLRPSLSLRLSNSLGEEEMKIHGTSFKNTPQTLAQSSPLVLSDNVPMLESDQKAAVTMGLSKSLEPDSPGSAPEATPKSPVRSEDFHSGMPLQGSPASPDKTQVVLPNKKPPPISKKPKLFLVVPPPQLDLTVEKIAEVSDTVRSTSSPTKRDAVLTHCEEARNCLTDGLSSSEMDSGSLVPEGGAARVTFSEAVGVGAFVVQSAASPVQEEPRQEEQSVFDEGSSSGSSQDSSSNTEGHPSQENESAEVFESETANSSFLPSNNYGEETDGVATPARPRTTEDLFAAIHRSKRKVLGRKDSEDDRTRNHSPSPPVTPTGASPTLSTLKQAGSIQRSVRKSSTSNDNFKALLLKKGSRCDTSSRMSAAEMLKHTDPRFHRAKVDASPDLSDSPASCSPSKSKRAQEEWAKSEGLMPRSMSLSSTRYGRSRTPPSAASSKYNVRNRIQSSPMTVISEGDGEVVEQTESRVQRTLDEQRERQLDVFNSDEIDMNDFSYSEELDCKETLDPTHLDLMTQPGTSRKYLSPSAEES

Target ORF information:

RefSeq Version	XM_025149171.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NHS like 1 (NHSL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025149171.1

ORF Insert Sequence:

ATGCCGTTCC CCCTGCGGAC CGTGGAGCCT CTGAAGCTGT GCAGGTTGGA GGAAGGCGAC 
GGCGCGGAGC GGGGCGGCCC GGCGCCGGGG GACCACGCCG GGGCGGCCGA AGGGGGCGGC 
GGGCGGCGGC GAGGCGCGGT GCCTCAGTTC GGGGCGCTGG AACAGGTCAG CTCGTACGCG 
CTGGTGTCGC TGCTCATGCA GCTCTCCGAC CTGTCCCGCT GCGCCGGAGA CATCTTCGGC 
GGGATCCTGA GCGAGGCGGA CACGCTGTGC CACCGCAACG CCCGGCTCCA GCGCCGTCTG 
GGAGCCCTCG AAGCTCTGCT CGCCCGCCTC GACCACCGCA AAGTGAAGAT CCCTGTTTCC 
AACTTAGATG AAGAGAGCAG ATGGACCGTC CATTACACTG CTCCATGGCA CCAGCAGGAA 
AATGTGTTCC TGCCTTCCTC AAGGCCGCCT TGTGTGGAGG ACTTGCACCG GCAAGCAAAG 
CTGAACCTCA AGTCAGTACT GAGAGAGTGT GACAAGCTGC GGAGAGATGG CTATCGGAGC 
TCTCAGTATT ACTCTCAGGG CCCCACCTTC TCCTCATCCT CCAGTGCAAT CTGTGGAAGT 
TACCAGGACG ATTATGAAGA AATTGAACAA AAGTGTTCTG TCTCTTCCCC AGAAGAAGAA 
AAGCTTATCA CCATCAAAAG GCCTAAAACC CCAGTTTCAA ATGACTTATC AGATATCAAC 
ACACAAACCA ACTGGACTAA GTCACTTCCA CTGCCAACAC CAGAAGAGAA GATGCGACAG 
CAAGCACAGG CAGTCCAAAC AGATGTGGTT CCTATCAATG TGACTGGGGA GAATTTCGAC 
CGCCAAGCCA GCATTCGGCG GTCTCTAATT TACACAGACA CGGTGGTAAG ACGGCCGAAG 
AAAGTCAAAA GGAGAAAGAC TATAACAGGA ATCCCTGACA ACATACAAAA GGAGCTAGCA 
GTTGGCACAG GCCAAAGTGA TTTCCGAGGG CACTCGATGT ATGTCCCAGA TCACAGTTCC 
ACGCTAGGGC GACTAGACAG CTATCGCTCT GCTATGCAGC GGTCTGAAAC CAAGGACACC 
AGCTGCCAGA CTGAAGAAGT TAAAGTTGTG CCTCCTTCGA TGAGAAGAAT ACGAGCACAG 
AAAGGACAAG GCATTGCAGC TCAGATGTCT CAGTTCTCCA GCTCATCTGG AAACATGTCT 
GTGATGAGTG ATTCTGCTGC AGTTATATTT GCTTCTCGCC AGAATAGTGA CATAGGTTTT 
CACAGCTTGC CTCGGGCTGG TGCAAGAGTG TCTCTGCAGT CCTTAGAACA GACCCAGAGC 
ATCTCCAGAC AGACAGAAGA CATTGCTGGT ACTTTGCCTC ACCAGATGAG TAAACTGCAA 
GTGGATGATG ATGTTGCACA TCTGAGAAAT AATGCCATAA CAGGAACTGT TTCGAGGCCA 
AAGTCTCAAG AGGTAAGAAG TTGTGACAGT GAAAAGTCCA CTAGCCCAGC ATGTGTGGTC 
TCTCCTCATG CCACCTACTC AACAAGCGTC ATACCCAATG CGACACTGTC ATCCTCCTCA 
GAAGTTATTG CTATTCACAC TGCCCAGAGT ACTGGATCGC TGGATAACAA AATTAGCAGT 
TCTGCTGTGT ACCCAAAACC AAGAGATAAT CCTGTTGCCA ACAATGCAAT AAGTGGGAAA 
GAAGATCACC ATTCTTCAAG TGGTAACTGG AGTGAGAGTA GCTCCACACG TCACTCACAG 
ACTTCAGATA CCATCCCATC TCATACTGTC ATGATGCTTT CTCTTGGTGA CTCTGCTGTC 
TCTCTCAGCA CTCCTGGAAA TGTAGAGGCT GGCTCTCAGA GTGTGAACTA CAGCTGCAGA 
AGCAATCTTG CTTCACCAAA CCAGTCCCAG GACAGCGATG GTAGGAGTGA ATCTAGCTAC 
TCAGGGGAGC GAACACAAGG TGTAGTGAGC AGCACAGAGC ACTGGCTATA CAAGTCTGCG 
GAAAATAGTG AGACTGCATC ATGCAAGGTA GCTTGTACCA CACCAGGCTG TGCCACTCCT 
GGTAGCAACC TGAGCAGCAG CAGCCTGGAG AGGACATCAG CCAAAGAAGA TTCCACTTCA 
CTGTATTCTG TAGACCACGA TGGTTATTAC AGCTCTATGA GCATGGACTC TGGACTGAAG 
TCAGACATGC CATGCTATAG TACTAATGGT TTTGGGAACC CCAGAGACAG CGTGATTAAT 
GTCTTTGAAG CAAAGGAGAA GAAACATCAA GATGACCAGC CAGGCCAAGG TGAAAAATCC 
CTTGCAAGAA ACATCTCTCT AAAAAAGGCA AAGAAGCCGC CTTTACCTCC GTCTAGAACA 
GACTCACTCA GAAGGGTACC CAAGAAGAAA GCTCAGTCCA ATGGACAGGT ACTTGATGAA 
ACCCTCATTG CTACCCTCCA GCATTCTCTG CAGTTGAATC TCAAGTGCAA AAATGGCAGC 
TCCCCTTCCC AGAGCCCCTG CAGTGACTAT GAGGATCCCT GGGTGTTGCG CTCTCGCAGC 
CAAAGCTCGG TCAGCGCAAG CAGCAGCATG ATGTCCACTA CTGCCCCAAA CCTGTACTCC 
ATTTGCACAG TCACTCCCTC TCAGAGTGAA ACAAGCAGCA TCAAGTCAGA GTATGCTGAC 
CAGTGGGGTT ACTACAGTGA CTATGCTGCA GTGGCAGATG ACCAGATGAA GTCTCCAGTG 
ACCCATTCTG CTAGTACCTC TTCTGCTCTC AGTGATTACA GCATCAGCCA CTTCAGTGAT 
GGCTCAAGGG CTTCTGTACC ACAAGTGCCC GCTGGACTGG CCAAACCAAA GAGCACTTCT 
CCAGAGAAGT CCCACCGAGT CACATCGCCA TCCAGTGGGT ACTCCAGCCA GTCCAATACA 
CCCACTGCAC TCACTCCAGT GCCTGTGTTT TTAAAATCTG CGTCATCAGG AAATGGGAAA 
TCCAAGCTGA AACCTAAAGT GCCTGAAAGG AAATCTTCTC TTCTGTCTTC AGTCTCCATT 
TCTTCATCCT CTACTTCTCT TTCTTCAAAT ACATCTACTG AAGGGAGTGT GAATGTGAAG 
AAATTGGACC CCACCCCGAG TTCTCCTCCA GATTCTGGTG CACCTCTTCC TCCGCCTCCT 
CTTCTAGCAC CTCCTCCACA TTGCCTTGAG CTCTCTCCTC CCTCTTCCCC TCCTCCTCCT 
CCAGCAGAAG CCGTGGATAT ATCACCATTG CCAGCTTCTC CCACATTCCC CCCCCCTCCA 
CCAGAAGCTG ATGTAAATTC TTCCTTTGCT CACAGTGTCC AGTGGTTACC ACAAGAAGCT 
TCCATCGGTT CGTACTCTTC CCCTGTTCCT CCTCCTGTTT TCCCTTTAGT TGTCCCACCA 
CCAGCCCCAC CTCTTGATCC CAAACTGACA AAAGGTGCAA TGATATGCCC ACAGTATTCA 
TTTAAGAAAC ATGATCAGGA AGATTCTTGC TACAATGCTG TGAAACAGCC ATTCAGCAAG 
CAAGATGCAT CAAGACCTGT GATGCCCTTG GTAACCACCA AAGCATTGCA AATGGTGCAG 
CTGAGGTCTA TCAGAAAAGC AACAGAAGGT GACCCATCAC CTGAATCTGC TTCTGAAACC 
ATCTCTCAGG AAAAGGGTAT TGTCACTTCA TCATCACAGT CTTCCCTAAG GCCATCTCTC 
TCACTACGAC TCAGTAACAG CTTAGGTGAA GAGGAGATGA AGATTCATGG CACTTCATTT 
AAAAACACAC CTCAAACACT GGCTCAGAGT TCTCCTCTGG TACTCTCTGA CAACGTACCC 
ATGCTTGAGA GCGATCAAAA AGCTGCAGTT ACTATGGGTC TAAGCAAGTC TTTGGAACCA 
GATTCCCCAG GGTCAGCTCC AGAAGCCACA CCCAAGTCCC CAGTTCGGAG CGAAGACTTC 
CATTCTGGCA TGCCACTGCA GGGGTCACCA GCATCTCCAG ACAAGACTCA GGTCGTTTTG 
CCAAACAAGA AACCACCTCC CATTTCAAAG AAACCCAAAC TGTTCCTTGT TGTACCACCT 
CCACAGTTAG ATCTCACAGT GGAGAAAATA GCTGAAGTGA GTGATACTGT CAGAAGCACA 
TCAAGCCCAA CTAAGAGAGA CGCTGTGCTT ACACACTGCG AGGAGGCGAG AAATTGCCTT 
ACAGATGGAC TCAGCTCCAG CGAGATGGAC TCTGGCAGCC TGGTTCCTGA GGGAGGAGCT 
GCCAGAGTCA CCTTCTCTGA AGCAGTGGGA GTTGGTGCCT TTGTGGTACA GTCCGCTGCA 
TCACCAGTTC AAGAGGAGCC CAGGCAAGAG GAGCAGTCAG TTTTTGATGA AGGAAGCAGC 
TCAGGCAGCA GCCAAGACAG CAGCAGTAAC ACTGAAGGAC ACCCGTCTCA GGAGAACGAA 
AGTGCCGAGG TGTTTGAATC AGAAACAGCA AATAGTTCAT TCCTGCCAAG CAATAATTAT 
GGGGAAGAGA CGGATGGAGT GGCAACACCA GCGAGACCGA GGACTACTGA GGATCTTTTT 
GCAGCCATTC ACAGATCCAA AAGGAAAGTC CTTGGCCGTA AGGATTCTGA GGACGATCGT 
ACCCGCAACC ATTCTCCTTC ACCCCCCGTA ACACCCACTG GTGCTTCCCC AACCTTATCT 
ACCCTCAAAC AGGCAGGATC TATTCAGAGA AGCGTTCGCA AGAGCAGCAC CAGCAATGAC 
AACTTCAAAG CCCTGCTGCT GAAGAAAGGG AGTCGCTGTG ACACCAGTTC CCGCATGTCA 
GCAGCAGAGA TGTTGAAGCA CACGGATCCA CGGTTCCACC GAGCCAAGGT GGATGCCTCT 
CCCGACCTTT CCGACAGCCC TGCCAGCTGC TCGCCCAGCA AGAGCAAACG GGCCCAAGAG 
GAGTGGGCCA AGAGTGAAGG CCTGATGCCG AGGAGCATGT CCCTCTCCAG CACTAGGTAT 
GGCCGATCAC GAACACCGCC CTCCGCTGCC AGCAGCAAGT ACAATGTTCG CAACCGCATC 
CAGAGCAGCC CCATGACAGT GATTAGCGAA GGAGATGGGG AAGTGGTGGA GCAGACAGAG 
AGCAGGGTCC AAAGGACTTT GGATGAGCAG CGAGAGAGGC AGCTCGATGT ATTTAACAGT 
GATGAAATAG ACATGAATGA CTTTTCGTAT TCTGAGGAGC TGGACTGCAA GGAGACCTTG 
GATCCAACCC ATCTGGACTT AATGACTCAG CCGGGTACTT CGAGAAAGTA CCTAAGTCCT 
TCAGCTGAAG AAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_015284192.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015139678.1 Gallus gallus NHS-like 1 (NHSL1), transcript variant X2, mRNA.

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CloneID	OGa33682
Clone ID Related Accession (Same CDS sequence)	XM_015284192.1 , XM_015284192.2
Accession Version	XM_015284192.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	NHS-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455837.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTTCC CCCTGCGGAC CGTGGAGCCT CTGAAGCTGT GCAGGTTGGA GGAAGGCGAC 
GGCGCGGAGC GGGGCGGCCC GGCGCCGGGG GACCACGCCG GGGCGGCCGA AGGGGGCGGC 
GGGCGGCGGC GAGGCGCGGT GCCTCAGTTC GGGGCGCTGG AACAGGTCAG CTCGTACGCG 
CTGGTGTCGC TGCTCATGCA GCTCTCCGAC CTGTCCCGCT GCGCCGGAGA CATCTTCGGC 
GGGATCCTGA GCGAGGCGGA CACGCTGTGC CACCGCAACG CCCGGCTCCA GCGCCGTCTG 
GGAGCCCTCG AAGCTCTGCT CGCCCGCCTC GACCACCGCA AAGTGAAGAT CCCTGTTTCC 
AACTTAGATG AAGAGAGCAG ATGGACCGTC CATTACACTG CTCCATGGCA CCAGCAGGAA 
AATGTGTTCC TGCCTTCCTC AAGGCCGCCT TGTGTGGAGG ACTTGCACCG GCAAGCAAAG 
CTGAACCTCA AGTCAGTACT GAGAGAGTGT GACAAGCTGC GGAGAGATGG CTATCGGAGC 
TCTCAGTATT ACTCTCAGGG CCCCACCTTC TCCTCATCCT CCAGTGCAAT CTGTGGAAGT 
TACCAGGACG ATTATGAAGA AATTGAACAA AAGTGTTCTG TCTCTTCCCC AGAAGAAGAA 
AAGCTTATCA CCATCAAAAG GCCTAAAACC CCAGTTTCAA ATGACTTATC AGATATCAAC 
ACACAAACCA ACTGGACTAA GTCACTTCCA CTGCCAACAC CAGAAGAGAA GATGCGACAG 
CAAGCACAGG CAGTCCAAAC AGATGTGGTT CCTATCAATG TGACTGGGGA GAATTTCGAC 
CGCCAAGCCA GCATTCGGCG GTCTCTAATT TACACAGACA CGGTGGTAAG ACGGCCGAAG 
AAAGTCAAAA GGAGAAAGAC TATAACAGGA ATCCCTGACA ACATACAAAA GGAGCTAGTT 
GGCACAGGCC AAAGTGATTT CCGAGGGCAC TCGATGTATG TCCCAGATCA CAGTTCCACG 
CTAGGGCGAC TAGACAGCTA TCGCTCTGCT ATGCAGCGGT CTGAAACCAA GGACACCAGC 
TGCCAGACTG AAGAAGTTAA AGTTGTGCCT CCTTCGATGA GAAGAATACG AGCACAGAAA 
GGACAAGGCA TTGCAGCTCA GATGTCTCAG TTCTCCAGCT CATCTGGAAA CATGTCTGTG 
ATGAGTGATT CTGCTGCAGT TATATTTGCT TCTCGCCAGA ATAGTGACAT AGGTTTTCAC 
AGCTTGCCTC GGGCTGGTGC AAGAGTGTCT CTGCAGTCCT TAGAACAGAC CCAGAGCATC 
TCCAGACAGA CAGAAGACAT TGCTGGTACT TTGCCTCACC AGATGAGTAA ACTGCAAGTG 
GATGATGATG TTGCACATCT GAGAAATAAT GCCATAACAG GAACTGTTTC GAGGCCAAAG 
TCTCAAGAGG TAAGAAGTTG TGACAGTGAA AAGTCCACTA GCCCAGCATG TGTGGTCTCT 
CCTCATGCCA CCTACTCAAC AAGCGTCATA CCCAATGCGA CACTGTCATC CTCCTCAGAA 
GTTATTGCTA TTCACACTGC CCAGAGTACT GGATCGCTGG ATAACAAAAT TAGCAGTTCT 
GCTGTGTACC CAAAACCAAG AGATAATCCT GTTGCCAACA ATGCAATAAG TGGGAAAGAA 
GATCACCATT CTTCAAGTGG TAACTGGAGT GAGAGTAGCT CCACACGTCA CTCACAGACT 
TCAGATACCA TCCCATCTCA TACTGTCATG ATGCTTTCTC TTGGTGACTC TGCTGTCTCT 
CTCAGCACTC CTGGAAATGT AGAGGCTGGC TCTCAGAGTG TGAACTACAG CTGCAGAAGC 
AATCTTGCTT CACCAAACCA GTCCCAGGAC AGCGATGGTA GGAGTGAATC TAGCTACTCA 
GGGGAGCGAA CACAAGGTGT AGTGAGCAGC ACAGAGCACT GGCTATACAA GTCTGCGGAA 
AATAGTGAGA CTGCATCATG CAAGGTAGCT TGTACCACAC CAGGCTGTGC CACTCCTGGT 
AGCAACCTGA GCAGCAGCAG CCTGGAGAGG ACATCAGCCA AAGAAGATTC CACTTCACTG 
TATTCTGTAG ACCACGATGG TTATTACAGC TCTATGAGCA TGGACTCTGG ACTGAAGTCA 
GACATGCCAT GCTATAGTAC TAATGGTTTT GGGAACCCCA GAGACAGCGT GATTAATGTC 
TTTGAAGCAA AGGAGAAGAA ACATCAAGAT GACCAGCCAG GCCAAGGTGA AAAATCCCTT 
GCAAGAAACA TCTCTCTAAA AAAGGCAAAG AAGCCGCCTT TACCTCCGTC TAGAACAGAC 
TCACTCAGAA GGGTACCCAA GAAGAAAGCT CAGTCCAATG GACAGGTACT TGATGAAACC 
CTCATTGCTA CCCTCCAGCA TTCTCTGCAG TTGAATCTCA AGTGCAAAAA TGGCAGCTCC 
CCTTCCCAGA GCCCCTGCAG TGACTATGAG GATCCCTGGG TGTTGCGCTC TCGCAGCCAA 
AGCTCGGTCA GCGCAAGCAG CAGCATGATG TCCACTACTG CCCCAAACCT GTACTCCATT 
TGCACAGTCA CTCCCTCTCA GAGTGAAACA AGCAGCATCA AGTCAGAGTA TGCTGACCAG 
TGGGGTTACT ACAGTGACTA TGCTGCAGTG GCAGATGACC AGATGAAGTC TCCAGTGACC 
CATTCTGCTA GTACCTCTTC TGCTCTCAGT GATTACAGCA TCAGCCACTT CAGTGATGGC 
TCAAGGGCTT CTGTACCACA AGTGCCCGCT GGACTGGCCA AACCAAAGAG CACTTCTCCA 
GAGAAGTCCC ACCGAGTCAC ATCGCCATCC AGTGGGTACT CCAGCCAGTC CAATACACCC 
ACTGCACTCA CTCCAGTGCC TGTGTTTTTA AAATCTGCGT CATCAGGAAA TGGGAAATCC 
AAGCTGAAAC CTAAAGTGCC TGAAAGGAAA TCTTCTCTTC TGTCTTCAGT CTCCATTTCT 
TCATCCTCTA CTTCTCTTTC TTCAAATACA TCTACTGAAG GGAGTGTGAA TGTGAAGAAA 
TTGGACCCCA CCCCGAGTTC TCCTCCAGAT TCTGGTGCAC CTCTTCCTCC GCCTCCTCTT 
CTAGCACCTC CTCCACATTG CCTTGAGCTC TCTCCTCCCT CTTCCCCTCC TCCTCCTCCA 
GCAGAAGCCG TGGATATATC ACCATTGCCA GCTTCTCCCA CATTCCCCCC CCCTCCACCA 
GAAGCTGATG TAAATTCTTC CTTTGCTCAC AGTGTCCAGT GGTTACCACA AGAAGCTTCC 
ATCGGTTCGT ACTCTTCCCC TGTTCCTCCT CCTGTTTTCC CTTTAGTTGT CCCACCACCA 
GCCCCACCTC TTGATCCCAA ACTGACAAAA GGTGCAATGA TATGCCCACA GTATTCATTT 
AAGAAACATG ATCAGGAAGA TTCTTGCTAC AATGCTGTGA AACAGCCATT CAGCAAGCAA 
GATGCATCAA GACCTGTGAT GCCCTTGGTA ACCACCAAAG CATTGCAAAT GGTGCAGCTG 
AGGTCTATCA GAAAAGCAAC AGAAGGTGAC CCATCACCTG AATCTGCTTC TGAAACCATC 
TCTCAGGAAA AGGGTATTGT CACTTCATCA TCACAGTCTT CCCTAAGGCC ATCTCTCTCA 
CTACGACTCA GTAACAGCTT AGGTGAAGAG GAGATGAAGA TTCATGGCAC TTCATTTAAA 
AACACACCTC AAACACTGGC TCAGAGTTCT CCTCTGGTAC TCTCTGACAA CGTACCCATG 
CTTGAGAGCG ATCAAAAAGC TGCAGTTACT ATGGGTCTAA GCAAGTCTTT GGAACCAGAT 
TCCCCAGGGT CAGCTCCAGA AGCCACACCC AAGTCCCCAG TTCGGAGCGA AGACTTCCAT 
TCTGGCATGC CACTGCAGGG GTCACCAGCA TCTCCAGACA AGACTCAGGT CGTTTTGCCA 
AACAAGAAAC CACCTCCCAT TTCAAAGAAA CCCAAACTGT TCCTTGTTGT ACCACCTCCA 
CAGTTAGATC TCACAGTGGA GAAAATAGCT GAAGTGAGTG ATACTGTCAG AAGCACATCA 
AGCCCAACTA AGAGAGACGC TGTGCTTACA CACTGCGAGG AGGCGAGAAA TTGCCTTACA 
GATGGACTCA GCTCCAGCGA GATGGACTCT GGCAGCCTGG TTCCTGAGGG AGGAGCTGCC 
AGAGTCACCT TCTCTGAAGC AGTGGGAGTT GGTGCCTTTG TGGTACAGTC CGCTGCATCA 
CCAGTTCAAG AGGAGCCCAG GCAAGAGGAG CAGTCAGTTT TTGATGAAGG AAGCAGCTCA 
GGCAGCAGCC AAGACAGCAG CAGTAACACT GAAGGACACC CGTCTCAGGA GAACGAAAGT 
GCCGAGGTGT TTGAATCAGA AACAGCAAAT AGTTCATTCC TGCCAAGCAA TAATTATGGG 
GAAGAGACGG ATGGAGTGGC AACACCAGCG AGACCGAGGA CTACTGAGGA TCTTTTTGCA 
GCCATTCACA GATCCAAAAG GAAAGTCCTT GGCCGTAAGG ATTCTGAGGA CGATCGTACC 
CGCAACCATT CTCCTTCACC CCCCGTAACA CCCACTGGTG CTTCCCCAAC CTTATCTACC 
CTCAAACAGG CAGGATCTAT TCAGAGAAGC GTTCGCAAGA GCAGCACCAG CAATGACAAC 
TTCAAAGCCC TGCTGCTGAA GAAAGGGAGT CGCTGTGACA CCAGTTCCCG CATGTCAGCA 
GCAGAGATGT TGAAGCACAC GGATCCACGG TTCCACCGAG CCAAGGTGGA TGCCTCTCCC 
GACCTTTCCG ACAGCCCTGC CAGCTGCTCG CCCAGCAAGA GCAAACGGGC CCAAGAGGAG 
TGGGCCAAGA GTGAAGGCCT GATGCCGAGG AGCATGTCCC TCTCCAGCAC TAGGTATGGC 
CGATCACGAA CACCGCCCTC CGCTGCCAGC AGCAAGTACA ATGTTCGCAA CCGCATCCAG 
AGCAGCCCCA TGACAGTGAT TAGCGAAGGA GATGGGGAAG TGGTGGAGCA GACAGAGAGC 
AGGGTCCAAA GGACTTTGGA TGAGCAGCGA GAGAGGCAGC TCGATGTATT TAACAGTGAT 
GAAATAGACA TGAATGACTT TTCGTATTCT GAGGAGCTGG ACTGCAAGGA GACCTTGGAT 
CCAACCCATC TGGACTTAAT GACTCAGCCG GGTACTTCGA GAAAGTACCT AAGTCCTTCA 
GCTGAAGAAA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015139678.1
CDS	95..5329
Translation	MPFPLRTVEPLKLCRLEEGDGAERGGPAPGDHAGAAEGGGGRRRGAVPQFGALEQVSSYALVSLLMQLSDLSRCAGDIFGGILSEADTLCHRNARLQRRLGALEALLARLDHRKVKIPVSNLDEESRWTVHYTAPWHQQENVFLPSSRPPCVEDLHRQAKLNLKSVLRECDKLRRDGYRSSQYYSQGPTFSSSSSAICGSYQDDYEEIEQKCSVSSPEEEKLITIKRPKTPVSNDLSDINTQTNWTKSLPLPTPEEKMRQQAQAVQTDVVPINVTGENFDRQASIRRSLIYTDTVVRRPKKVKRRKTITGIPDNIQKELVGTGQSDFRGHSMYVPDHSSTLGRLDSYRSAMQRSETKDTSCQTEEVKVVPPSMRRIRAQKGQGIAAQMSQFSSSSGNMSVMSDSAAVIFASRQNSDIGFHSLPRAGARVSLQSLEQTQSISRQTEDIAGTLPHQMSKLQVDDDVAHLRNNAITGTVSRPKSQEVRSCDSEKSTSPACVVSPHATYSTSVIPNATLSSSSEVIAIHTAQSTGSLDNKISSSAVYPKPRDNPVANNAISGKEDHHSSSGNWSESSSTRHSQTSDTIPSHTVMMLSLGDSAVSLSTPGNVEAGSQSVNYSCRSNLASPNQSQDSDGRSESSYSGERTQGVVSSTEHWLYKSAENSETASCKVACTTPGCATPGSNLSSSSLERTSAKEDSTSLYSVDHDGYYSSMSMDSGLKSDMPCYSTNGFGNPRDSVINVFEAKEKKHQDDQPGQGEKSLARNISLKKAKKPPLPPSRTDSLRRVPKKKAQSNGQVLDETLIATLQHSLQLNLKCKNGSSPSQSPCSDYEDPWVLRSRSQSSVSASSSMMSTTAPNLYSICTVTPSQSETSSIKSEYADQWGYYSDYAAVADDQMKSPVTHSASTSSALSDYSISHFSDGSRASVPQVPAGLAKPKSTSPEKSHRVTSPSSGYSSQSNTPTALTPVPVFLKSASSGNGKSKLKPKVPERKSSLLSSVSISSSSTSLSSNTSTEGSVNVKKLDPTPSSPPDSGAPLPPPPLLAPPPHCLELSPPSSPPPPPAEAVDISPLPASPTFPPPPPEADVNSSFAHSVQWLPQEASIGSYSSPVPPPVFPLVVPPPAPPLDPKLTKGAMICPQYSFKKHDQEDSCYNAVKQPFSKQDASRPVMPLVTTKALQMVQLRSIRKATEGDPSPESASETISQEKGIVTSSSQSSLRPSLSLRLSNSLGEEEMKIHGTSFKNTPQTLAQSSPLVLSDNVPMLESDQKAAVTMGLSKSLEPDSPGSAPEATPKSPVRSEDFHSGMPLQGSPASPDKTQVVLPNKKPPPISKKPKLFLVVPPPQLDLTVEKIAEVSDTVRSTSSPTKRDAVLTHCEEARNCLTDGLSSSEMDSGSLVPEGGAARVTFSEAVGVGAFVVQSAASPVQEEPRQEEQSVFDEGSSSGSSQDSSSNTEGHPSQENESAEVFESETANSSFLPSNNYGEETDGVATPARPRTTEDLFAAIHRSKRKVLGRKDSEDDRTRNHSPSPPVTPTGASPTLSTLKQAGSIQRSVRKSSTSNDNFKALLLKKGSRCDTSSRMSAAEMLKHTDPRFHRAKVDASPDLSDSPASCSPSKSKRAQEEWAKSEGLMPRSMSLSSTRYGRSRTPPSAASSKYNVRNRIQSSPMTVISEGDGEVVEQTESRVQRTLDEQRERQLDVFNSDEIDMNDFSYSEELDCKETLDPTHLDLMTQPGTSRKYLSPSAEES

Target ORF information:

RefSeq Version	XM_015284192.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NHS-like 1 (NHSL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015284192.1

ORF Insert Sequence:

ATGCCGTTCC CCCTGCGGAC CGTGGAGCCT CTGAAGCTGT GCAGGTTGGA GGAAGGCGAC 
GGCGCGGAGC GGGGCGGCCC GGCGCCGGGG GACCACGCCG GGGCGGCCGA AGGGGGCGGC 
GGGCGGCGGC GAGGCGCGGT GCCTCAGTTC GGGGCGCTGG AACAGGTCAG CTCGTACGCG 
CTGGTGTCGC TGCTCATGCA GCTCTCCGAC CTGTCCCGCT GCGCCGGAGA CATCTTCGGC 
GGGATCCTGA GCGAGGCGGA CACGCTGTGC CACCGCAACG CCCGGCTCCA GCGCCGTCTG 
GGAGCCCTCG AAGCTCTGCT CGCCCGCCTC GACCACCGCA AAGTGAAGAT CCCTGTTTCC 
AACTTAGATG AAGAGAGCAG ATGGACCGTC CATTACACTG CTCCATGGCA CCAGCAGGAA 
AATGTGTTCC TGCCTTCCTC AAGGCCGCCT TGTGTGGAGG ACTTGCACCG GCAAGCAAAG 
CTGAACCTCA AGTCAGTACT GAGAGAGTGT GACAAGCTGC GGAGAGATGG CTATCGGAGC 
TCTCAGTATT ACTCTCAGGG CCCCACCTTC TCCTCATCCT CCAGTGCAAT CTGTGGAAGT 
TACCAGGACG ATTATGAAGA AATTGAACAA AAGTGTTCTG TCTCTTCCCC AGAAGAAGAA 
AAGCTTATCA CCATCAAAAG GCCTAAAACC CCAGTTTCAA ATGACTTATC AGATATCAAC 
ACACAAACCA ACTGGACTAA GTCACTTCCA CTGCCAACAC CAGAAGAGAA GATGCGACAG 
CAAGCACAGG CAGTCCAAAC AGATGTGGTT CCTATCAATG TGACTGGGGA GAATTTCGAC 
CGCCAAGCCA GCATTCGGCG GTCTCTAATT TACACAGACA CGGTGGTAAG ACGGCCGAAG 
AAAGTCAAAA GGAGAAAGAC TATAACAGGA ATCCCTGACA ACATACAAAA GGAGCTAGTT 
GGCACAGGCC AAAGTGATTT CCGAGGGCAC TCGATGTATG TCCCAGATCA CAGTTCCACG 
CTAGGGCGAC TAGACAGCTA TCGCTCTGCT ATGCAGCGGT CTGAAACCAA GGACACCAGC 
TGCCAGACTG AAGAAGTTAA AGTTGTGCCT CCTTCGATGA GAAGAATACG AGCACAGAAA 
GGACAAGGCA TTGCAGCTCA GATGTCTCAG TTCTCCAGCT CATCTGGAAA CATGTCTGTG 
ATGAGTGATT CTGCTGCAGT TATATTTGCT TCTCGCCAGA ATAGTGACAT AGGTTTTCAC 
AGCTTGCCTC GGGCTGGTGC AAGAGTGTCT CTGCAGTCCT TAGAACAGAC CCAGAGCATC 
TCCAGACAGA CAGAAGACAT TGCTGGTACT TTGCCTCACC AGATGAGTAA ACTGCAAGTG 
GATGATGATG TTGCACATCT GAGAAATAAT GCCATAACAG GAACTGTTTC GAGGCCAAAG 
TCTCAAGAGG TAAGAAGTTG TGACAGTGAA AAGTCCACTA GCCCAGCATG TGTGGTCTCT 
CCTCATGCCA CCTACTCAAC AAGCGTCATA CCCAATGCGA CACTGTCATC CTCCTCAGAA 
GTTATTGCTA TTCACACTGC CCAGAGTACT GGATCGCTGG ATAACAAAAT TAGCAGTTCT 
GCTGTGTACC CAAAACCAAG AGATAATCCT GTTGCCAACA ATGCAATAAG TGGGAAAGAA 
GATCACCATT CTTCAAGTGG TAACTGGAGT GAGAGTAGCT CCACACGTCA CTCACAGACT 
TCAGATACCA TCCCATCTCA TACTGTCATG ATGCTTTCTC TTGGTGACTC TGCTGTCTCT 
CTCAGCACTC CTGGAAATGT AGAGGCTGGC TCTCAGAGTG TGAACTACAG CTGCAGAAGC 
AATCTTGCTT CACCAAACCA GTCCCAGGAC AGCGATGGTA GGAGTGAATC TAGCTACTCA 
GGGGAGCGAA CACAAGGTGT AGTGAGCAGC ACAGAGCACT GGCTATACAA GTCTGCGGAA 
AATAGTGAGA CTGCATCATG CAAGGTAGCT TGTACCACAC CAGGCTGTGC CACTCCTGGT 
AGCAACCTGA GCAGCAGCAG CCTGGAGAGG ACATCAGCCA AAGAAGATTC CACTTCACTG 
TATTCTGTAG ACCACGATGG TTATTACAGC TCTATGAGCA TGGACTCTGG ACTGAAGTCA 
GACATGCCAT GCTATAGTAC TAATGGTTTT GGGAACCCCA GAGACAGCGT GATTAATGTC 
TTTGAAGCAA AGGAGAAGAA ACATCAAGAT GACCAGCCAG GCCAAGGTGA AAAATCCCTT 
GCAAGAAACA TCTCTCTAAA AAAGGCAAAG AAGCCGCCTT TACCTCCGTC TAGAACAGAC 
TCACTCAGAA GGGTACCCAA GAAGAAAGCT CAGTCCAATG GACAGGTACT TGATGAAACC 
CTCATTGCTA CCCTCCAGCA TTCTCTGCAG TTGAATCTCA AGTGCAAAAA TGGCAGCTCC 
CCTTCCCAGA GCCCCTGCAG TGACTATGAG GATCCCTGGG TGTTGCGCTC TCGCAGCCAA 
AGCTCGGTCA GCGCAAGCAG CAGCATGATG TCCACTACTG CCCCAAACCT GTACTCCATT 
TGCACAGTCA CTCCCTCTCA GAGTGAAACA AGCAGCATCA AGTCAGAGTA TGCTGACCAG 
TGGGGTTACT ACAGTGACTA TGCTGCAGTG GCAGATGACC AGATGAAGTC TCCAGTGACC 
CATTCTGCTA GTACCTCTTC TGCTCTCAGT GATTACAGCA TCAGCCACTT CAGTGATGGC 
TCAAGGGCTT CTGTACCACA AGTGCCCGCT GGACTGGCCA AACCAAAGAG CACTTCTCCA 
GAGAAGTCCC ACCGAGTCAC ATCGCCATCC AGTGGGTACT CCAGCCAGTC CAATACACCC 
ACTGCACTCA CTCCAGTGCC TGTGTTTTTA AAATCTGCGT CATCAGGAAA TGGGAAATCC 
AAGCTGAAAC CTAAAGTGCC TGAAAGGAAA TCTTCTCTTC TGTCTTCAGT CTCCATTTCT 
TCATCCTCTA CTTCTCTTTC TTCAAATACA TCTACTGAAG GGAGTGTGAA TGTGAAGAAA 
TTGGACCCCA CCCCGAGTTC TCCTCCAGAT TCTGGTGCAC CTCTTCCTCC GCCTCCTCTT 
CTAGCACCTC CTCCACATTG CCTTGAGCTC TCTCCTCCCT CTTCCCCTCC TCCTCCTCCA 
GCAGAAGCCG TGGATATATC ACCATTGCCA GCTTCTCCCA CATTCCCCCC CCCTCCACCA 
GAAGCTGATG TAAATTCTTC CTTTGCTCAC AGTGTCCAGT GGTTACCACA AGAAGCTTCC 
ATCGGTTCGT ACTCTTCCCC TGTTCCTCCT CCTGTTTTCC CTTTAGTTGT CCCACCACCA 
GCCCCACCTC TTGATCCCAA ACTGACAAAA GGTGCAATGA TATGCCCACA GTATTCATTT 
AAGAAACATG ATCAGGAAGA TTCTTGCTAC AATGCTGTGA AACAGCCATT CAGCAAGCAA 
GATGCATCAA GACCTGTGAT GCCCTTGGTA ACCACCAAAG CATTGCAAAT GGTGCAGCTG 
AGGTCTATCA GAAAAGCAAC AGAAGGTGAC CCATCACCTG AATCTGCTTC TGAAACCATC 
TCTCAGGAAA AGGGTATTGT CACTTCATCA TCACAGTCTT CCCTAAGGCC ATCTCTCTCA 
CTACGACTCA GTAACAGCTT AGGTGAAGAG GAGATGAAGA TTCATGGCAC TTCATTTAAA 
AACACACCTC AAACACTGGC TCAGAGTTCT CCTCTGGTAC TCTCTGACAA CGTACCCATG 
CTTGAGAGCG ATCAAAAAGC TGCAGTTACT ATGGGTCTAA GCAAGTCTTT GGAACCAGAT 
TCCCCAGGGT CAGCTCCAGA AGCCACACCC AAGTCCCCAG TTCGGAGCGA AGACTTCCAT 
TCTGGCATGC CACTGCAGGG GTCACCAGCA TCTCCAGACA AGACTCAGGT CGTTTTGCCA 
AACAAGAAAC CACCTCCCAT TTCAAAGAAA CCCAAACTGT TCCTTGTTGT ACCACCTCCA 
CAGTTAGATC TCACAGTGGA GAAAATAGCT GAAGTGAGTG ATACTGTCAG AAGCACATCA 
AGCCCAACTA AGAGAGACGC TGTGCTTACA CACTGCGAGG AGGCGAGAAA TTGCCTTACA 
GATGGACTCA GCTCCAGCGA GATGGACTCT GGCAGCCTGG TTCCTGAGGG AGGAGCTGCC 
AGAGTCACCT TCTCTGAAGC AGTGGGAGTT GGTGCCTTTG TGGTACAGTC CGCTGCATCA 
CCAGTTCAAG AGGAGCCCAG GCAAGAGGAG CAGTCAGTTT TTGATGAAGG AAGCAGCTCA 
GGCAGCAGCC AAGACAGCAG CAGTAACACT GAAGGACACC CGTCTCAGGA GAACGAAAGT 
GCCGAGGTGT TTGAATCAGA AACAGCAAAT AGTTCATTCC TGCCAAGCAA TAATTATGGG 
GAAGAGACGG ATGGAGTGGC AACACCAGCG AGACCGAGGA CTACTGAGGA TCTTTTTGCA 
GCCATTCACA GATCCAAAAG GAAAGTCCTT GGCCGTAAGG ATTCTGAGGA CGATCGTACC 
CGCAACCATT CTCCTTCACC CCCCGTAACA CCCACTGGTG CTTCCCCAAC CTTATCTACC 
CTCAAACAGG CAGGATCTAT TCAGAGAAGC GTTCGCAAGA GCAGCACCAG CAATGACAAC 
TTCAAAGCCC TGCTGCTGAA GAAAGGGAGT CGCTGTGACA CCAGTTCCCG CATGTCAGCA 
GCAGAGATGT TGAAGCACAC GGATCCACGG TTCCACCGAG CCAAGGTGGA TGCCTCTCCC 
GACCTTTCCG ACAGCCCTGC CAGCTGCTCG CCCAGCAAGA GCAAACGGGC CCAAGAGGAG 
TGGGCCAAGA GTGAAGGCCT GATGCCGAGG AGCATGTCCC TCTCCAGCAC TAGGTATGGC 
CGATCACGAA CACCGCCCTC CGCTGCCAGC AGCAAGTACA ATGTTCGCAA CCGCATCCAG 
AGCAGCCCCA TGACAGTGAT TAGCGAAGGA GATGGGGAAG TGGTGGAGCA GACAGAGAGC 
AGGGTCCAAA GGACTTTGGA TGAGCAGCGA GAGAGGCAGC TCGATGTATT TAACAGTGAT 
GAAATAGACA TGAATGACTT TTCGTATTCT GAGGAGCTGG ACTGCAAGGA GACCTTGGAT 
CCAACCCATC TGGACTTAAT GACTCAGCCG GGTACTTCGA GAAAGTACCT AAGTCCTTCA 
GCTGAAGAAA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_015284192.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015139678.1 Gallus gallus NHS like 1 (NHSL1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa33682
Clone ID Related Accession (Same CDS sequence)	XM_015284192.1 , XM_015284192.2
Accession Version	XM_015284192.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	NHS-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015284192.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTTCC CCCTGCGGAC CGTGGAGCCT CTGAAGCTGT GCAGGTTGGA GGAAGGCGAC 
GGCGCGGAGC GGGGCGGCCC GGCGCCGGGG GACCACGCCG GGGCGGCCGA AGGGGGCGGC 
GGGCGGCGGC GAGGCGCGGT GCCTCAGTTC GGGGCGCTGG AACAGGTCAG CTCGTACGCG 
CTGGTGTCGC TGCTCATGCA GCTCTCCGAC CTGTCCCGCT GCGCCGGAGA CATCTTCGGC 
GGGATCCTGA GCGAGGCGGA CACGCTGTGC CACCGCAACG CCCGGCTCCA GCGCCGTCTG 
GGAGCCCTCG AAGCTCTGCT CGCCCGCCTC GACCACCGCA AAGTGAAGAT CCCTGTTTCC 
AACTTAGATG AAGAGAGCAG ATGGACCGTC CATTACACTG CTCCATGGCA CCAGCAGGAA 
AATGTGTTCC TGCCTTCCTC AAGGCCGCCT TGTGTGGAGG ACTTGCACCG GCAAGCAAAG 
CTGAACCTCA AGTCAGTACT GAGAGAGTGT GACAAGCTGC GGAGAGATGG CTATCGGAGC 
TCTCAGTATT ACTCTCAGGG CCCCACCTTC TCCTCATCCT CCAGTGCAAT CTGTGGAAGT 
TACCAGGACG ATTATGAAGA AATTGAACAA AAGTGTTCTG TCTCTTCCCC AGAAGAAGAA 
AAGCTTATCA CCATCAAAAG GCCTAAAACC CCAGTTTCAA ATGACTTATC AGATATCAAC 
ACACAAACCA ACTGGACTAA GTCACTTCCA CTGCCAACAC CAGAAGAGAA GATGCGACAG 
CAAGCACAGG CAGTCCAAAC AGATGTGGTT CCTATCAATG TGACTGGGGA GAATTTCGAC 
CGCCAAGCCA GCATTCGGCG GTCTCTAATT TACACAGACA CGGTGGTAAG ACGGCCGAAG 
AAAGTCAAAA GGAGAAAGAC TATAACAGGA ATCCCTGACA ACATACAAAA GGAGCTAGTT 
GGCACAGGCC AAAGTGATTT CCGAGGGCAC TCGATGTATG TCCCAGATCA CAGTTCCACG 
CTAGGGCGAC TAGACAGCTA TCGCTCTGCT ATGCAGCGGT CTGAAACCAA GGACACCAGC 
TGCCAGACTG AAGAAGTTAA AGTTGTGCCT CCTTCGATGA GAAGAATACG AGCACAGAAA 
GGACAAGGCA TTGCAGCTCA GATGTCTCAG TTCTCCAGCT CATCTGGAAA CATGTCTGTG 
ATGAGTGATT CTGCTGCAGT TATATTTGCT TCTCGCCAGA ATAGTGACAT AGGTTTTCAC 
AGCTTGCCTC GGGCTGGTGC AAGAGTGTCT CTGCAGTCCT TAGAACAGAC CCAGAGCATC 
TCCAGACAGA CAGAAGACAT TGCTGGTACT TTGCCTCACC AGATGAGTAA ACTGCAAGTG 
GATGATGATG TTGCACATCT GAGAAATAAT GCCATAACAG GAACTGTTTC GAGGCCAAAG 
TCTCAAGAGG TAAGAAGTTG TGACAGTGAA AAGTCCACTA GCCCAGCATG TGTGGTCTCT 
CCTCATGCCA CCTACTCAAC AAGCGTCATA CCCAATGCGA CACTGTCATC CTCCTCAGAA 
GTTATTGCTA TTCACACTGC CCAGAGTACT GGATCGCTGG ATAACAAAAT TAGCAGTTCT 
GCTGTGTACC CAAAACCAAG AGATAATCCT GTTGCCAACA ATGCAATAAG TGGGAAAGAA 
GATCACCATT CTTCAAGTGG TAACTGGAGT GAGAGTAGCT CCACACGTCA CTCACAGACT 
TCAGATACCA TCCCATCTCA TACTGTCATG ATGCTTTCTC TTGGTGACTC TGCTGTCTCT 
CTCAGCACTC CTGGAAATGT AGAGGCTGGC TCTCAGAGTG TGAACTACAG CTGCAGAAGC 
AATCTTGCTT CACCAAACCA GTCCCAGGAC AGCGATGGTA GGAGTGAATC TAGCTACTCA 
GGGGAGCGAA CACAAGGTGT AGTGAGCAGC ACAGAGCACT GGCTATACAA GTCTGCGGAA 
AATAGTGAGA CTGCATCATG CAAGGTAGCT TGTACCACAC CAGGCTGTGC CACTCCTGGT 
AGCAACCTGA GCAGCAGCAG CCTGGAGAGG ACATCAGCCA AAGAAGATTC CACTTCACTG 
TATTCTGTAG ACCACGATGG TTATTACAGC TCTATGAGCA TGGACTCTGG ACTGAAGTCA 
GACATGCCAT GCTATAGTAC TAATGGTTTT GGGAACCCCA GAGACAGCGT GATTAATGTC 
TTTGAAGCAA AGGAGAAGAA ACATCAAGAT GACCAGCCAG GCCAAGGTGA AAAATCCCTT 
GCAAGAAACA TCTCTCTAAA AAAGGCAAAG AAGCCGCCTT TACCTCCGTC TAGAACAGAC 
TCACTCAGAA GGGTACCCAA GAAGAAAGCT CAGTCCAATG GACAGGTACT TGATGAAACC 
CTCATTGCTA CCCTCCAGCA TTCTCTGCAG TTGAATCTCA AGTGCAAAAA TGGCAGCTCC 
CCTTCCCAGA GCCCCTGCAG TGACTATGAG GATCCCTGGG TGTTGCGCTC TCGCAGCCAA 
AGCTCGGTCA GCGCAAGCAG CAGCATGATG TCCACTACTG CCCCAAACCT GTACTCCATT 
TGCACAGTCA CTCCCTCTCA GAGTGAAACA AGCAGCATCA AGTCAGAGTA TGCTGACCAG 
TGGGGTTACT ACAGTGACTA TGCTGCAGTG GCAGATGACC AGATGAAGTC TCCAGTGACC 
CATTCTGCTA GTACCTCTTC TGCTCTCAGT GATTACAGCA TCAGCCACTT CAGTGATGGC 
TCAAGGGCTT CTGTACCACA AGTGCCCGCT GGACTGGCCA AACCAAAGAG CACTTCTCCA 
GAGAAGTCCC ACCGAGTCAC ATCGCCATCC AGTGGGTACT CCAGCCAGTC CAATACACCC 
ACTGCACTCA CTCCAGTGCC TGTGTTTTTA AAATCTGCGT CATCAGGAAA TGGGAAATCC 
AAGCTGAAAC CTAAAGTGCC TGAAAGGAAA TCTTCTCTTC TGTCTTCAGT CTCCATTTCT 
TCATCCTCTA CTTCTCTTTC TTCAAATACA TCTACTGAAG GGAGTGTGAA TGTGAAGAAA 
TTGGACCCCA CCCCGAGTTC TCCTCCAGAT TCTGGTGCAC CTCTTCCTCC GCCTCCTCTT 
CTAGCACCTC CTCCACATTG CCTTGAGCTC TCTCCTCCCT CTTCCCCTCC TCCTCCTCCA 
GCAGAAGCCG TGGATATATC ACCATTGCCA GCTTCTCCCA CATTCCCCCC CCCTCCACCA 
GAAGCTGATG TAAATTCTTC CTTTGCTCAC AGTGTCCAGT GGTTACCACA AGAAGCTTCC 
ATCGGTTCGT ACTCTTCCCC TGTTCCTCCT CCTGTTTTCC CTTTAGTTGT CCCACCACCA 
GCCCCACCTC TTGATCCCAA ACTGACAAAA GGTGCAATGA TATGCCCACA GTATTCATTT 
AAGAAACATG ATCAGGAAGA TTCTTGCTAC AATGCTGTGA AACAGCCATT CAGCAAGCAA 
GATGCATCAA GACCTGTGAT GCCCTTGGTA ACCACCAAAG CATTGCAAAT GGTGCAGCTG 
AGGTCTATCA GAAAAGCAAC AGAAGGTGAC CCATCACCTG AATCTGCTTC TGAAACCATC 
TCTCAGGAAA AGGGTATTGT CACTTCATCA TCACAGTCTT CCCTAAGGCC ATCTCTCTCA 
CTACGACTCA GTAACAGCTT AGGTGAAGAG GAGATGAAGA TTCATGGCAC TTCATTTAAA 
AACACACCTC AAACACTGGC TCAGAGTTCT CCTCTGGTAC TCTCTGACAA CGTACCCATG 
CTTGAGAGCG ATCAAAAAGC TGCAGTTACT ATGGGTCTAA GCAAGTCTTT GGAACCAGAT 
TCCCCAGGGT CAGCTCCAGA AGCCACACCC AAGTCCCCAG TTCGGAGCGA AGACTTCCAT 
TCTGGCATGC CACTGCAGGG GTCACCAGCA TCTCCAGACA AGACTCAGGT CGTTTTGCCA 
AACAAGAAAC CACCTCCCAT TTCAAAGAAA CCCAAACTGT TCCTTGTTGT ACCACCTCCA 
CAGTTAGATC TCACAGTGGA GAAAATAGCT GAAGTGAGTG ATACTGTCAG AAGCACATCA 
AGCCCAACTA AGAGAGACGC TGTGCTTACA CACTGCGAGG AGGCGAGAAA TTGCCTTACA 
GATGGACTCA GCTCCAGCGA GATGGACTCT GGCAGCCTGG TTCCTGAGGG AGGAGCTGCC 
AGAGTCACCT TCTCTGAAGC AGTGGGAGTT GGTGCCTTTG TGGTACAGTC CGCTGCATCA 
CCAGTTCAAG AGGAGCCCAG GCAAGAGGAG CAGTCAGTTT TTGATGAAGG AAGCAGCTCA 
GGCAGCAGCC AAGACAGCAG CAGTAACACT GAAGGACACC CGTCTCAGGA GAACGAAAGT 
GCCGAGGTGT TTGAATCAGA AACAGCAAAT AGTTCATTCC TGCCAAGCAA TAATTATGGG 
GAAGAGACGG ATGGAGTGGC AACACCAGCG AGACCGAGGA CTACTGAGGA TCTTTTTGCA 
GCCATTCACA GATCCAAAAG GAAAGTCCTT GGCCGTAAGG ATTCTGAGGA CGATCGTACC 
CGCAACCATT CTCCTTCACC CCCCGTAACA CCCACTGGTG CTTCCCCAAC CTTATCTACC 
CTCAAACAGG CAGGATCTAT TCAGAGAAGC GTTCGCAAGA GCAGCACCAG CAATGACAAC 
TTCAAAGCCC TGCTGCTGAA GAAAGGGAGT CGCTGTGACA CCAGTTCCCG CATGTCAGCA 
GCAGAGATGT TGAAGCACAC GGATCCACGG TTCCACCGAG CCAAGGTGGA TGCCTCTCCC 
GACCTTTCCG ACAGCCCTGC CAGCTGCTCG CCCAGCAAGA GCAAACGGGC CCAAGAGGAG 
TGGGCCAAGA GTGAAGGCCT GATGCCGAGG AGCATGTCCC TCTCCAGCAC TAGGTATGGC 
CGATCACGAA CACCGCCCTC CGCTGCCAGC AGCAAGTACA ATGTTCGCAA CCGCATCCAG 
AGCAGCCCCA TGACAGTGAT TAGCGAAGGA GATGGGGAAG TGGTGGAGCA GACAGAGAGC 
AGGGTCCAAA GGACTTTGGA TGAGCAGCGA GAGAGGCAGC TCGATGTATT TAACAGTGAT 
GAAATAGACA TGAATGACTT TTCGTATTCT GAGGAGCTGG ACTGCAAGGA GACCTTGGAT 
CCAACCCATC TGGACTTAAT GACTCAGCCG GGTACTTCGA GAAAGTACCT AAGTCCTTCA 
GCTGAAGAAA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015139678.1
CDS	34..5268
Translation	MPFPLRTVEPLKLCRLEEGDGAERGGPAPGDHAGAAEGGGGRRRGAVPQFGALEQVSSYALVSLLMQLSDLSRCAGDIFGGILSEADTLCHRNARLQRRLGALEALLARLDHRKVKIPVSNLDEESRWTVHYTAPWHQQENVFLPSSRPPCVEDLHRQAKLNLKSVLRECDKLRRDGYRSSQYYSQGPTFSSSSSAICGSYQDDYEEIEQKCSVSSPEEEKLITIKRPKTPVSNDLSDINTQTNWTKSLPLPTPEEKMRQQAQAVQTDVVPINVTGENFDRQASIRRSLIYTDTVVRRPKKVKRRKTITGIPDNIQKELVGTGQSDFRGHSMYVPDHSSTLGRLDSYRSAMQRSETKDTSCQTEEVKVVPPSMRRIRAQKGQGIAAQMSQFSSSSGNMSVMSDSAAVIFASRQNSDIGFHSLPRAGARVSLQSLEQTQSISRQTEDIAGTLPHQMSKLQVDDDVAHLRNNAITGTVSRPKSQEVRSCDSEKSTSPACVVSPHATYSTSVIPNATLSSSSEVIAIHTAQSTGSLDNKISSSAVYPKPRDNPVANNAISGKEDHHSSSGNWSESSSTRHSQTSDTIPSHTVMMLSLGDSAVSLSTPGNVEAGSQSVNYSCRSNLASPNQSQDSDGRSESSYSGERTQGVVSSTEHWLYKSAENSETASCKVACTTPGCATPGSNLSSSSLERTSAKEDSTSLYSVDHDGYYSSMSMDSGLKSDMPCYSTNGFGNPRDSVINVFEAKEKKHQDDQPGQGEKSLARNISLKKAKKPPLPPSRTDSLRRVPKKKAQSNGQVLDETLIATLQHSLQLNLKCKNGSSPSQSPCSDYEDPWVLRSRSQSSVSASSSMMSTTAPNLYSICTVTPSQSETSSIKSEYADQWGYYSDYAAVADDQMKSPVTHSASTSSALSDYSISHFSDGSRASVPQVPAGLAKPKSTSPEKSHRVTSPSSGYSSQSNTPTALTPVPVFLKSASSGNGKSKLKPKVPERKSSLLSSVSISSSSTSLSSNTSTEGSVNVKKLDPTPSSPPDSGAPLPPPPLLAPPPHCLELSPPSSPPPPPAEAVDISPLPASPTFPPPPPEADVNSSFAHSVQWLPQEASIGSYSSPVPPPVFPLVVPPPAPPLDPKLTKGAMICPQYSFKKHDQEDSCYNAVKQPFSKQDASRPVMPLVTTKALQMVQLRSIRKATEGDPSPESASETISQEKGIVTSSSQSSLRPSLSLRLSNSLGEEEMKIHGTSFKNTPQTLAQSSPLVLSDNVPMLESDQKAAVTMGLSKSLEPDSPGSAPEATPKSPVRSEDFHSGMPLQGSPASPDKTQVVLPNKKPPPISKKPKLFLVVPPPQLDLTVEKIAEVSDTVRSTSSPTKRDAVLTHCEEARNCLTDGLSSSEMDSGSLVPEGGAARVTFSEAVGVGAFVVQSAASPVQEEPRQEEQSVFDEGSSSGSSQDSSSNTEGHPSQENESAEVFESETANSSFLPSNNYGEETDGVATPARPRTTEDLFAAIHRSKRKVLGRKDSEDDRTRNHSPSPPVTPTGASPTLSTLKQAGSIQRSVRKSSTSNDNFKALLLKKGSRCDTSSRMSAAEMLKHTDPRFHRAKVDASPDLSDSPASCSPSKSKRAQEEWAKSEGLMPRSMSLSSTRYGRSRTPPSAASSKYNVRNRIQSSPMTVISEGDGEVVEQTESRVQRTLDEQRERQLDVFNSDEIDMNDFSYSEELDCKETLDPTHLDLMTQPGTSRKYLSPSAEES

Target ORF information:

RefSeq Version	XM_015284192.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NHS like 1 (NHSL1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015284192.2

ORF Insert Sequence:

ATGCCGTTCC CCCTGCGGAC CGTGGAGCCT CTGAAGCTGT GCAGGTTGGA GGAAGGCGAC 
GGCGCGGAGC GGGGCGGCCC GGCGCCGGGG GACCACGCCG GGGCGGCCGA AGGGGGCGGC 
GGGCGGCGGC GAGGCGCGGT GCCTCAGTTC GGGGCGCTGG AACAGGTCAG CTCGTACGCG 
CTGGTGTCGC TGCTCATGCA GCTCTCCGAC CTGTCCCGCT GCGCCGGAGA CATCTTCGGC 
GGGATCCTGA GCGAGGCGGA CACGCTGTGC CACCGCAACG CCCGGCTCCA GCGCCGTCTG 
GGAGCCCTCG AAGCTCTGCT CGCCCGCCTC GACCACCGCA AAGTGAAGAT CCCTGTTTCC 
AACTTAGATG AAGAGAGCAG ATGGACCGTC CATTACACTG CTCCATGGCA CCAGCAGGAA 
AATGTGTTCC TGCCTTCCTC AAGGCCGCCT TGTGTGGAGG ACTTGCACCG GCAAGCAAAG 
CTGAACCTCA AGTCAGTACT GAGAGAGTGT GACAAGCTGC GGAGAGATGG CTATCGGAGC 
TCTCAGTATT ACTCTCAGGG CCCCACCTTC TCCTCATCCT CCAGTGCAAT CTGTGGAAGT 
TACCAGGACG ATTATGAAGA AATTGAACAA AAGTGTTCTG TCTCTTCCCC AGAAGAAGAA 
AAGCTTATCA CCATCAAAAG GCCTAAAACC CCAGTTTCAA ATGACTTATC AGATATCAAC 
ACACAAACCA ACTGGACTAA GTCACTTCCA CTGCCAACAC CAGAAGAGAA GATGCGACAG 
CAAGCACAGG CAGTCCAAAC AGATGTGGTT CCTATCAATG TGACTGGGGA GAATTTCGAC 
CGCCAAGCCA GCATTCGGCG GTCTCTAATT TACACAGACA CGGTGGTAAG ACGGCCGAAG 
AAAGTCAAAA GGAGAAAGAC TATAACAGGA ATCCCTGACA ACATACAAAA GGAGCTAGTT 
GGCACAGGCC AAAGTGATTT CCGAGGGCAC TCGATGTATG TCCCAGATCA CAGTTCCACG 
CTAGGGCGAC TAGACAGCTA TCGCTCTGCT ATGCAGCGGT CTGAAACCAA GGACACCAGC 
TGCCAGACTG AAGAAGTTAA AGTTGTGCCT CCTTCGATGA GAAGAATACG AGCACAGAAA 
GGACAAGGCA TTGCAGCTCA GATGTCTCAG TTCTCCAGCT CATCTGGAAA CATGTCTGTG 
ATGAGTGATT CTGCTGCAGT TATATTTGCT TCTCGCCAGA ATAGTGACAT AGGTTTTCAC 
AGCTTGCCTC GGGCTGGTGC AAGAGTGTCT CTGCAGTCCT TAGAACAGAC CCAGAGCATC 
TCCAGACAGA CAGAAGACAT TGCTGGTACT TTGCCTCACC AGATGAGTAA ACTGCAAGTG 
GATGATGATG TTGCACATCT GAGAAATAAT GCCATAACAG GAACTGTTTC GAGGCCAAAG 
TCTCAAGAGG TAAGAAGTTG TGACAGTGAA AAGTCCACTA GCCCAGCATG TGTGGTCTCT 
CCTCATGCCA CCTACTCAAC AAGCGTCATA CCCAATGCGA CACTGTCATC CTCCTCAGAA 
GTTATTGCTA TTCACACTGC CCAGAGTACT GGATCGCTGG ATAACAAAAT TAGCAGTTCT 
GCTGTGTACC CAAAACCAAG AGATAATCCT GTTGCCAACA ATGCAATAAG TGGGAAAGAA 
GATCACCATT CTTCAAGTGG TAACTGGAGT GAGAGTAGCT CCACACGTCA CTCACAGACT 
TCAGATACCA TCCCATCTCA TACTGTCATG ATGCTTTCTC TTGGTGACTC TGCTGTCTCT 
CTCAGCACTC CTGGAAATGT AGAGGCTGGC TCTCAGAGTG TGAACTACAG CTGCAGAAGC 
AATCTTGCTT CACCAAACCA GTCCCAGGAC AGCGATGGTA GGAGTGAATC TAGCTACTCA 
GGGGAGCGAA CACAAGGTGT AGTGAGCAGC ACAGAGCACT GGCTATACAA GTCTGCGGAA 
AATAGTGAGA CTGCATCATG CAAGGTAGCT TGTACCACAC CAGGCTGTGC CACTCCTGGT 
AGCAACCTGA GCAGCAGCAG CCTGGAGAGG ACATCAGCCA AAGAAGATTC CACTTCACTG 
TATTCTGTAG ACCACGATGG TTATTACAGC TCTATGAGCA TGGACTCTGG ACTGAAGTCA 
GACATGCCAT GCTATAGTAC TAATGGTTTT GGGAACCCCA GAGACAGCGT GATTAATGTC 
TTTGAAGCAA AGGAGAAGAA ACATCAAGAT GACCAGCCAG GCCAAGGTGA AAAATCCCTT 
GCAAGAAACA TCTCTCTAAA AAAGGCAAAG AAGCCGCCTT TACCTCCGTC TAGAACAGAC 
TCACTCAGAA GGGTACCCAA GAAGAAAGCT CAGTCCAATG GACAGGTACT TGATGAAACC 
CTCATTGCTA CCCTCCAGCA TTCTCTGCAG TTGAATCTCA AGTGCAAAAA TGGCAGCTCC 
CCTTCCCAGA GCCCCTGCAG TGACTATGAG GATCCCTGGG TGTTGCGCTC TCGCAGCCAA 
AGCTCGGTCA GCGCAAGCAG CAGCATGATG TCCACTACTG CCCCAAACCT GTACTCCATT 
TGCACAGTCA CTCCCTCTCA GAGTGAAACA AGCAGCATCA AGTCAGAGTA TGCTGACCAG 
TGGGGTTACT ACAGTGACTA TGCTGCAGTG GCAGATGACC AGATGAAGTC TCCAGTGACC 
CATTCTGCTA GTACCTCTTC TGCTCTCAGT GATTACAGCA TCAGCCACTT CAGTGATGGC 
TCAAGGGCTT CTGTACCACA AGTGCCCGCT GGACTGGCCA AACCAAAGAG CACTTCTCCA 
GAGAAGTCCC ACCGAGTCAC ATCGCCATCC AGTGGGTACT CCAGCCAGTC CAATACACCC 
ACTGCACTCA CTCCAGTGCC TGTGTTTTTA AAATCTGCGT CATCAGGAAA TGGGAAATCC 
AAGCTGAAAC CTAAAGTGCC TGAAAGGAAA TCTTCTCTTC TGTCTTCAGT CTCCATTTCT 
TCATCCTCTA CTTCTCTTTC TTCAAATACA TCTACTGAAG GGAGTGTGAA TGTGAAGAAA 
TTGGACCCCA CCCCGAGTTC TCCTCCAGAT TCTGGTGCAC CTCTTCCTCC GCCTCCTCTT 
CTAGCACCTC CTCCACATTG CCTTGAGCTC TCTCCTCCCT CTTCCCCTCC TCCTCCTCCA 
GCAGAAGCCG TGGATATATC ACCATTGCCA GCTTCTCCCA CATTCCCCCC CCCTCCACCA 
GAAGCTGATG TAAATTCTTC CTTTGCTCAC AGTGTCCAGT GGTTACCACA AGAAGCTTCC 
ATCGGTTCGT ACTCTTCCCC TGTTCCTCCT CCTGTTTTCC CTTTAGTTGT CCCACCACCA 
GCCCCACCTC TTGATCCCAA ACTGACAAAA GGTGCAATGA TATGCCCACA GTATTCATTT 
AAGAAACATG ATCAGGAAGA TTCTTGCTAC AATGCTGTGA AACAGCCATT CAGCAAGCAA 
GATGCATCAA GACCTGTGAT GCCCTTGGTA ACCACCAAAG CATTGCAAAT GGTGCAGCTG 
AGGTCTATCA GAAAAGCAAC AGAAGGTGAC CCATCACCTG AATCTGCTTC TGAAACCATC 
TCTCAGGAAA AGGGTATTGT CACTTCATCA TCACAGTCTT CCCTAAGGCC ATCTCTCTCA 
CTACGACTCA GTAACAGCTT AGGTGAAGAG GAGATGAAGA TTCATGGCAC TTCATTTAAA 
AACACACCTC AAACACTGGC TCAGAGTTCT CCTCTGGTAC TCTCTGACAA CGTACCCATG 
CTTGAGAGCG ATCAAAAAGC TGCAGTTACT ATGGGTCTAA GCAAGTCTTT GGAACCAGAT 
TCCCCAGGGT CAGCTCCAGA AGCCACACCC AAGTCCCCAG TTCGGAGCGA AGACTTCCAT 
TCTGGCATGC CACTGCAGGG GTCACCAGCA TCTCCAGACA AGACTCAGGT CGTTTTGCCA 
AACAAGAAAC CACCTCCCAT TTCAAAGAAA CCCAAACTGT TCCTTGTTGT ACCACCTCCA 
CAGTTAGATC TCACAGTGGA GAAAATAGCT GAAGTGAGTG ATACTGTCAG AAGCACATCA 
AGCCCAACTA AGAGAGACGC TGTGCTTACA CACTGCGAGG AGGCGAGAAA TTGCCTTACA 
GATGGACTCA GCTCCAGCGA GATGGACTCT GGCAGCCTGG TTCCTGAGGG AGGAGCTGCC 
AGAGTCACCT TCTCTGAAGC AGTGGGAGTT GGTGCCTTTG TGGTACAGTC CGCTGCATCA 
CCAGTTCAAG AGGAGCCCAG GCAAGAGGAG CAGTCAGTTT TTGATGAAGG AAGCAGCTCA 
GGCAGCAGCC AAGACAGCAG CAGTAACACT GAAGGACACC CGTCTCAGGA GAACGAAAGT 
GCCGAGGTGT TTGAATCAGA AACAGCAAAT AGTTCATTCC TGCCAAGCAA TAATTATGGG 
GAAGAGACGG ATGGAGTGGC AACACCAGCG AGACCGAGGA CTACTGAGGA TCTTTTTGCA 
GCCATTCACA GATCCAAAAG GAAAGTCCTT GGCCGTAAGG ATTCTGAGGA CGATCGTACC 
CGCAACCATT CTCCTTCACC CCCCGTAACA CCCACTGGTG CTTCCCCAAC CTTATCTACC 
CTCAAACAGG CAGGATCTAT TCAGAGAAGC GTTCGCAAGA GCAGCACCAG CAATGACAAC 
TTCAAAGCCC TGCTGCTGAA GAAAGGGAGT CGCTGTGACA CCAGTTCCCG CATGTCAGCA 
GCAGAGATGT TGAAGCACAC GGATCCACGG TTCCACCGAG CCAAGGTGGA TGCCTCTCCC 
GACCTTTCCG ACAGCCCTGC CAGCTGCTCG CCCAGCAAGA GCAAACGGGC CCAAGAGGAG 
TGGGCCAAGA GTGAAGGCCT GATGCCGAGG AGCATGTCCC TCTCCAGCAC TAGGTATGGC 
CGATCACGAA CACCGCCCTC CGCTGCCAGC AGCAAGTACA ATGTTCGCAA CCGCATCCAG 
AGCAGCCCCA TGACAGTGAT TAGCGAAGGA GATGGGGAAG TGGTGGAGCA GACAGAGAGC 
AGGGTCCAAA GGACTTTGGA TGAGCAGCGA GAGAGGCAGC TCGATGTATT TAACAGTGAT 
GAAATAGACA TGAATGACTT TTCGTATTCT GAGGAGCTGG ACTGCAAGGA GACCTTGGAT 
CCAACCCATC TGGACTTAAT GACTCAGCCG GGTACTTCGA GAAAGTACCT AAGTCCTTCA 
GCTGAAGAAA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_015284193.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015139679.1 Gallus gallus NHS like 1 (NHSL1), transcript variant X4, mRNA.

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CloneID	OGa33683
Clone ID Related Accession (Same CDS sequence)	XM_015284193.2 , XM_015284193.1
Accession Version	XM_015284193.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	NHS-like protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015284193.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTTCC CCCTGCGGAC CGTGGAGCCT CTGAAGCTGT GCAGGTTGGA GGAAGGCGAC 
GGCGCGGAGC GGGGCGGCCC GGCGCCGGGG GACCACGCCG GGGCGGCCGA AGGGGGCGGC 
GGGCGGCGGC GAGGCGCGGT GCCTCAGTTC GGGGCGCTGG AACAGGTCAG CTCGTACGCG 
CTGGTGTCGC TGCTCATGCA GCTCTCCGAC CTGTCCCGCT GCGCCGGAGA CATCTTCGGC 
GGGATCCTGA GCGAGGCGGA CACGCTGTGC CACCGCAACG CCCGGCTCCA GCGCCGTCTG 
GGAGCCCTCG AAGCTCTGCT CGCCCGCCTC GACCACCGCA AAGTGAAGAT CCCTGTTTCC 
AACTTAGATG AAGAGAGCAG ATGGACCGTC CATTACACTG CTCCATGGCA CCAGCAGGAA 
AATGTGTTCC TGCCTTCCTC AAGGCCGCCT TGTGTGGAGG ACTTGCACCG GCAAGCAAAG 
CTGAACCTCA AGTCAGTACT GAGAGAGTGT GACAAGCTGC GGAGAGATGG CTATCGGAGC 
TCTCAGTATT ACTCTCAGGG CCCCACCTTC TCCTCATCCT CCAGTGCAAT CTGTGGAAGT 
TACCAGGACG ATTATGAAGA AATTGAACAA AAGTGTTCTG TCTCTTCCCC AGAAGAAGAA 
AAGCTTATCA CCATCAAAAG GCCTAAAACC CCAGTTTCAA ATGACTTATC AGATATCAAC 
ACACAAACCA ACTGGACTAA GTCACTTCCA CTGCCAACAC CAGAAGAGAA GATGCGACAG 
CAAGCACAGG CAGTCCAAAC AGATGTGGTT CCTATCAATG TGACTGCAGT TGGCACAGGC 
CAAAGTGATT TCCGAGGGCA CTCGATGTAT GTCCCAGATC ACAGTTCCAC GCTAGGGCGA 
CTAGACAGCT ATCGCTCTGC TATGCAGCGG TCTGAAACCA AGGACACCAG CTGCCAGACT 
GAAGAAGTTA AAGTTGTGCC TCCTTCGATG AGAAGAATAC GAGCACAGAA AGGACAAGGC 
ATTGCAGCTC AGATGTCTCA GTTCTCCAGC TCATCTGGAA ACATGTCTGT GATGAGTGAT 
TCTGCTGCAG TTATATTTGC TTCTCGCCAG AATAGTGACA TAGGTTTTCA CAGCTTGCCT 
CGGGCTGGTG CAAGAGTGTC TCTGCAGTCC TTAGAACAGA CCCAGAGCAT CTCCAGACAG 
ACAGAAGACA TTGCTGGTAC TTTGCCTCAC CAGATGAGTA AACTGCAAGT GGATGATGAT 
GTTGCACATC TGAGAAATAA TGCCATAACA GGAACTGTTT CGAGGCCAAA GTCTCAAGAG 
GTAAGAAGTT GTGACAGTGA AAAGTCCACT AGCCCAGCAT GTGTGGTCTC TCCTCATGCC 
ACCTACTCAA CAAGCGTCAT ACCCAATGCG ACACTGTCAT CCTCCTCAGA AGTTATTGCT 
ATTCACACTG CCCAGAGTAC TGGATCGCTG GATAACAAAA TTAGCAGTTC TGCTGTGTAC 
CCAAAACCAA GAGATAATCC TGTTGCCAAC AATGCAATAA GTGGGAAAGA AGATCACCAT 
TCTTCAAGTG GTAACTGGAG TGAGAGTAGC TCCACACGTC ACTCACAGAC TTCAGATACC 
ATCCCATCTC ATACTGTCAT GATGCTTTCT CTTGGTGACT CTGCTGTCTC TCTCAGCACT 
CCTGGAAATG TAGAGGCTGG CTCTCAGAGT GTGAACTACA GCTGCAGAAG CAATCTTGCT 
TCACCAAACC AGTCCCAGGA CAGCGATGGT AGGAGTGAAT CTAGCTACTC AGGGGAGCGA 
ACACAAGGTG TAGTGAGCAG CACAGAGCAC TGGCTATACA AGTCTGCGGA AAATAGTGAG 
ACTGCATCAT GCAAGGTAGC TTGTACCACA CCAGGCTGTG CCACTCCTGG TAGCAACCTG 
AGCAGCAGCA GCCTGGAGAG GACATCAGCC AAAGAAGATT CCACTTCACT GTATTCTGTA 
GACCACGATG GTTATTACAG CTCTATGAGC ATGGACTCTG GACTGAAGTC AGACATGCCA 
TGCTATAGTA CTAATGGTTT TGGGAACCCC AGAGACAGCG TGATTAATGT CTTTGAAGCA 
AAGGAGAAGA AACATCAAGA TGACCAGCCA GGCCAAGGTG AAAAATCCCT TGCAAGAAAC 
ATCTCTCTAA AAAAGGCAAA GAAGCCGCCT TTACCTCCGT CTAGAACAGA CTCACTCAGA 
AGGGTACCCA AGAAGAAAGC TCAGTCCAAT GGACAGGTAC TTGATGAAAC CCTCATTGCT 
ACCCTCCAGC ATTCTCTGCA GTTGAATCTC AAGTGCAAAA ATGGCAGCTC CCCTTCCCAG 
AGCCCCTGCA GTGACTATGA GGATCCCTGG GTGTTGCGCT CTCGCAGCCA AAGCTCGGTC 
AGCGCAAGCA GCAGCATGAT GTCCACTACT GCCCCAAACC TGTACTCCAT TTGCACAGTC 
ACTCCCTCTC AGAGTGAAAC AAGCAGCATC AAGTCAGAGT ATGCTGACCA GTGGGGTTAC 
TACAGTGACT ATGCTGCAGT GGCAGATGAC CAGATGAAGT CTCCAGTGAC CCATTCTGCT 
AGTACCTCTT CTGCTCTCAG TGATTACAGC ATCAGCCACT TCAGTGATGG CTCAAGGGCT 
TCTGTACCAC AAGTGCCCGC TGGACTGGCC AAACCAAAGA GCACTTCTCC AGAGAAGTCC 
CACCGAGTCA CATCGCCATC CAGTGGGTAC TCCAGCCAGT CCAATACACC CACTGCACTC 
ACTCCAGTGC CTGTGTTTTT AAAATCTGCG TCATCAGGAA ATGGGAAATC CAAGCTGAAA 
CCTAAAGTGC CTGAAAGGAA ATCTTCTCTT CTGTCTTCAG TCTCCATTTC TTCATCCTCT 
ACTTCTCTTT CTTCAAATAC ATCTACTGAA GGGAGTGTGA ATGTGAAGAA ATTGGACCCC 
ACCCCGAGTT CTCCTCCAGA TTCTGGTGCA CCTCTTCCTC CGCCTCCTCT TCTAGCACCT 
CCTCCACATT GCCTTGAGCT CTCTCCTCCC TCTTCCCCTC CTCCTCCTCC AGCAGAAGCC 
GTGGATATAT CACCATTGCC AGCTTCTCCC ACATTCCCCC CCCCTCCACC AGAAGCTGAT 
GTAAATTCTT CCTTTGCTCA CAGTGTCCAG TGGTTACCAC AAGAAGCTTC CATCGGTTCG 
TACTCTTCCC CTGTTCCTCC TCCTGTTTTC CCTTTAGTTG TCCCACCACC AGCCCCACCT 
CTTGATCCCA AACTGACAAA AGGTGCAATG ATATGCCCAC AGTATTCATT TAAGAAACAT 
GATCAGGAAG ATTCTTGCTA CAATGCTGTG AAACAGCCAT TCAGCAAGCA AGATGCATCA 
AGACCTGTGA TGCCCTTGGT AACCACCAAA GCATTGCAAA TGGTGCAGCT GAGGTCTATC 
AGAAAAGCAA CAGAAGGTGA CCCATCACCT GAATCTGCTT CTGAAACCAT CTCTCAGGAA 
AAGGGTATTG TCACTTCATC ATCACAGTCT TCCCTAAGGC CATCTCTCTC ACTACGACTC 
AGTAACAGCT TAGGTGAAGA GGAGATGAAG ATTCATGGCA CTTCATTTAA AAACACACCT 
CAAACACTGG CTCAGAGTTC TCCTCTGGTA CTCTCTGACA ACGTACCCAT GCTTGAGAGC 
GATCAAAAAG CTGCAGTTAC TATGGGTCTA AGCAAGTCTT TGGAACCAGA TTCCCCAGGG 
TCAGCTCCAG AAGCCACACC CAAGTCCCCA GTTCGGAGCG AAGACTTCCA TTCTGGCATG 
CCACTGCAGG GGTCACCAGC ATCTCCAGAC AAGACTCAGG TCGTTTTGCC AAACAAGAAA 
CCACCTCCCA TTTCAAAGAA ACCCAAACTG TTCCTTGTTG TACCACCTCC ACAGTTAGAT 
CTCACAGTGG AGAAAATAGC TGAAGTGAGT GATACTGTCA GAAGCACATC AAGCCCAACT 
AAGAGAGACG CTGTGCTTAC ACACTGCGAG GAGGCGAGAA ATTGCCTTAC AGATGGACTC 
AGCTCCAGCG AGATGGACTC TGGCAGCCTG GTTCCTGAGG GAGGAGCTGC CAGAGTCACC 
TTCTCTGAAG CAGTGGGAGT TGGTGCCTTT GTGGTACAGT CCGCTGCATC ACCAGTTCAA 
GAGGAGCCCA GGCAAGAGGA GCAGTCAGTT TTTGATGAAG GAAGCAGCTC AGGCAGCAGC 
CAAGACAGCA GCAGTAACAC TGAAGGACAC CCGTCTCAGG AGAACGAAAG TGCCGAGGTG 
TTTGAATCAG AAACAGCAAA TAGTTCATTC CTGCCAAGCA ATAATTATGG GGAAGAGACG 
GATGGAGTGG CAACACCAGC GAGACCGAGG ACTACTGAGG ATCTTTTTGC AGCCATTCAC 
AGATCCAAAA GGAAAGTCCT TGGCCGTAAG GATTCTGAGG ACGATCGTAC CCGCAACCAT 
TCTCCTTCAC CCCCCGTAAC ACCCACTGGT GCTTCCCCAA CCTTATCTAC CCTCAAACAG 
GCAGGATCTA TTCAGAGAAG CGTTCGCAAG AGCAGCACCA GCAATGACAA CTTCAAAGCC 
CTGCTGCTGA AGAAAGGGAG TCGCTGTGAC ACCAGTTCCC GCATGTCAGC AGCAGAGATG 
TTGAAGCACA CGGATCCACG GTTCCACCGA GCCAAGGTGG ATGCCTCTCC CGACCTTTCC 
GACAGCCCTG CCAGCTGCTC GCCCAGCAAG AGCAAACGGG CCCAAGAGGA GTGGGCCAAG 
AGTGAAGGCC TGATGCCGAG GAGCATGTCC CTCTCCAGCA CTAGGTATGG CCGATCACGA 
ACACCGCCCT CCGCTGCCAG CAGCAAGTAC AATGTTCGCA ACCGCATCCA GAGCAGCCCC 
ATGACAGTGA TTAGCGAAGG AGATGGGGAA GTGGTGGAGC AGACAGAGAG CAGGGTCCAA 
AGGACTTTGG ATGAGCAGCG AGAGAGGCAG CTCGATGTAT TTAACAGTGA TGAAATAGAC 
ATGAATGACT TTTCGTATTC TGAGGAGCTG GACTGCAAGG AGACCTTGGA TCCAACCCAT 
CTGGACTTAA TGACTCAGCC GGGTACTTCG AGAAAGTACC TAAGTCCTTC AGCTGAAGAA 
AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015139679.1
CDS	34..5139
Translation	MPFPLRTVEPLKLCRLEEGDGAERGGPAPGDHAGAAEGGGGRRRGAVPQFGALEQVSSYALVSLLMQLSDLSRCAGDIFGGILSEADTLCHRNARLQRRLGALEALLARLDHRKVKIPVSNLDEESRWTVHYTAPWHQQENVFLPSSRPPCVEDLHRQAKLNLKSVLRECDKLRRDGYRSSQYYSQGPTFSSSSSAICGSYQDDYEEIEQKCSVSSPEEEKLITIKRPKTPVSNDLSDINTQTNWTKSLPLPTPEEKMRQQAQAVQTDVVPINVTAVGTGQSDFRGHSMYVPDHSSTLGRLDSYRSAMQRSETKDTSCQTEEVKVVPPSMRRIRAQKGQGIAAQMSQFSSSSGNMSVMSDSAAVIFASRQNSDIGFHSLPRAGARVSLQSLEQTQSISRQTEDIAGTLPHQMSKLQVDDDVAHLRNNAITGTVSRPKSQEVRSCDSEKSTSPACVVSPHATYSTSVIPNATLSSSSEVIAIHTAQSTGSLDNKISSSAVYPKPRDNPVANNAISGKEDHHSSSGNWSESSSTRHSQTSDTIPSHTVMMLSLGDSAVSLSTPGNVEAGSQSVNYSCRSNLASPNQSQDSDGRSESSYSGERTQGVVSSTEHWLYKSAENSETASCKVACTTPGCATPGSNLSSSSLERTSAKEDSTSLYSVDHDGYYSSMSMDSGLKSDMPCYSTNGFGNPRDSVINVFEAKEKKHQDDQPGQGEKSLARNISLKKAKKPPLPPSRTDSLRRVPKKKAQSNGQVLDETLIATLQHSLQLNLKCKNGSSPSQSPCSDYEDPWVLRSRSQSSVSASSSMMSTTAPNLYSICTVTPSQSETSSIKSEYADQWGYYSDYAAVADDQMKSPVTHSASTSSALSDYSISHFSDGSRASVPQVPAGLAKPKSTSPEKSHRVTSPSSGYSSQSNTPTALTPVPVFLKSASSGNGKSKLKPKVPERKSSLLSSVSISSSSTSLSSNTSTEGSVNVKKLDPTPSSPPDSGAPLPPPPLLAPPPHCLELSPPSSPPPPPAEAVDISPLPASPTFPPPPPEADVNSSFAHSVQWLPQEASIGSYSSPVPPPVFPLVVPPPAPPLDPKLTKGAMICPQYSFKKHDQEDSCYNAVKQPFSKQDASRPVMPLVTTKALQMVQLRSIRKATEGDPSPESASETISQEKGIVTSSSQSSLRPSLSLRLSNSLGEEEMKIHGTSFKNTPQTLAQSSPLVLSDNVPMLESDQKAAVTMGLSKSLEPDSPGSAPEATPKSPVRSEDFHSGMPLQGSPASPDKTQVVLPNKKPPPISKKPKLFLVVPPPQLDLTVEKIAEVSDTVRSTSSPTKRDAVLTHCEEARNCLTDGLSSSEMDSGSLVPEGGAARVTFSEAVGVGAFVVQSAASPVQEEPRQEEQSVFDEGSSSGSSQDSSSNTEGHPSQENESAEVFESETANSSFLPSNNYGEETDGVATPARPRTTEDLFAAIHRSKRKVLGRKDSEDDRTRNHSPSPPVTPTGASPTLSTLKQAGSIQRSVRKSSTSNDNFKALLLKKGSRCDTSSRMSAAEMLKHTDPRFHRAKVDASPDLSDSPASCSPSKSKRAQEEWAKSEGLMPRSMSLSSTRYGRSRTPPSAASSKYNVRNRIQSSPMTVISEGDGEVVEQTESRVQRTLDEQRERQLDVFNSDEIDMNDFSYSEELDCKETLDPTHLDLMTQPGTSRKYLSPSAEES

Target ORF information:

RefSeq Version	XM_015284193.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NHS like 1 (NHSL1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015284193.2

ORF Insert Sequence:

ATGCCGTTCC CCCTGCGGAC CGTGGAGCCT CTGAAGCTGT GCAGGTTGGA GGAAGGCGAC 
GGCGCGGAGC GGGGCGGCCC GGCGCCGGGG GACCACGCCG GGGCGGCCGA AGGGGGCGGC 
GGGCGGCGGC GAGGCGCGGT GCCTCAGTTC GGGGCGCTGG AACAGGTCAG CTCGTACGCG 
CTGGTGTCGC TGCTCATGCA GCTCTCCGAC CTGTCCCGCT GCGCCGGAGA CATCTTCGGC 
GGGATCCTGA GCGAGGCGGA CACGCTGTGC CACCGCAACG CCCGGCTCCA GCGCCGTCTG 
GGAGCCCTCG AAGCTCTGCT CGCCCGCCTC GACCACCGCA AAGTGAAGAT CCCTGTTTCC 
AACTTAGATG AAGAGAGCAG ATGGACCGTC CATTACACTG CTCCATGGCA CCAGCAGGAA 
AATGTGTTCC TGCCTTCCTC AAGGCCGCCT TGTGTGGAGG ACTTGCACCG GCAAGCAAAG 
CTGAACCTCA AGTCAGTACT GAGAGAGTGT GACAAGCTGC GGAGAGATGG CTATCGGAGC 
TCTCAGTATT ACTCTCAGGG CCCCACCTTC TCCTCATCCT CCAGTGCAAT CTGTGGAAGT 
TACCAGGACG ATTATGAAGA AATTGAACAA AAGTGTTCTG TCTCTTCCCC AGAAGAAGAA 
AAGCTTATCA CCATCAAAAG GCCTAAAACC CCAGTTTCAA ATGACTTATC AGATATCAAC 
ACACAAACCA ACTGGACTAA GTCACTTCCA CTGCCAACAC CAGAAGAGAA GATGCGACAG 
CAAGCACAGG CAGTCCAAAC AGATGTGGTT CCTATCAATG TGACTGCAGT TGGCACAGGC 
CAAAGTGATT TCCGAGGGCA CTCGATGTAT GTCCCAGATC ACAGTTCCAC GCTAGGGCGA 
CTAGACAGCT ATCGCTCTGC TATGCAGCGG TCTGAAACCA AGGACACCAG CTGCCAGACT 
GAAGAAGTTA AAGTTGTGCC TCCTTCGATG AGAAGAATAC GAGCACAGAA AGGACAAGGC 
ATTGCAGCTC AGATGTCTCA GTTCTCCAGC TCATCTGGAA ACATGTCTGT GATGAGTGAT 
TCTGCTGCAG TTATATTTGC TTCTCGCCAG AATAGTGACA TAGGTTTTCA CAGCTTGCCT 
CGGGCTGGTG CAAGAGTGTC TCTGCAGTCC TTAGAACAGA CCCAGAGCAT CTCCAGACAG 
ACAGAAGACA TTGCTGGTAC TTTGCCTCAC CAGATGAGTA AACTGCAAGT GGATGATGAT 
GTTGCACATC TGAGAAATAA TGCCATAACA GGAACTGTTT CGAGGCCAAA GTCTCAAGAG 
GTAAGAAGTT GTGACAGTGA AAAGTCCACT AGCCCAGCAT GTGTGGTCTC TCCTCATGCC 
ACCTACTCAA CAAGCGTCAT ACCCAATGCG ACACTGTCAT CCTCCTCAGA AGTTATTGCT 
ATTCACACTG CCCAGAGTAC TGGATCGCTG GATAACAAAA TTAGCAGTTC TGCTGTGTAC 
CCAAAACCAA GAGATAATCC TGTTGCCAAC AATGCAATAA GTGGGAAAGA AGATCACCAT 
TCTTCAAGTG GTAACTGGAG TGAGAGTAGC TCCACACGTC ACTCACAGAC TTCAGATACC 
ATCCCATCTC ATACTGTCAT GATGCTTTCT CTTGGTGACT CTGCTGTCTC TCTCAGCACT 
CCTGGAAATG TAGAGGCTGG CTCTCAGAGT GTGAACTACA GCTGCAGAAG CAATCTTGCT 
TCACCAAACC AGTCCCAGGA CAGCGATGGT AGGAGTGAAT CTAGCTACTC AGGGGAGCGA 
ACACAAGGTG TAGTGAGCAG CACAGAGCAC TGGCTATACA AGTCTGCGGA AAATAGTGAG 
ACTGCATCAT GCAAGGTAGC TTGTACCACA CCAGGCTGTG CCACTCCTGG TAGCAACCTG 
AGCAGCAGCA GCCTGGAGAG GACATCAGCC AAAGAAGATT CCACTTCACT GTATTCTGTA 
GACCACGATG GTTATTACAG CTCTATGAGC ATGGACTCTG GACTGAAGTC AGACATGCCA 
TGCTATAGTA CTAATGGTTT TGGGAACCCC AGAGACAGCG TGATTAATGT CTTTGAAGCA 
AAGGAGAAGA AACATCAAGA TGACCAGCCA GGCCAAGGTG AAAAATCCCT TGCAAGAAAC 
ATCTCTCTAA AAAAGGCAAA GAAGCCGCCT TTACCTCCGT CTAGAACAGA CTCACTCAGA 
AGGGTACCCA AGAAGAAAGC TCAGTCCAAT GGACAGGTAC TTGATGAAAC CCTCATTGCT 
ACCCTCCAGC ATTCTCTGCA GTTGAATCTC AAGTGCAAAA ATGGCAGCTC CCCTTCCCAG 
AGCCCCTGCA GTGACTATGA GGATCCCTGG GTGTTGCGCT CTCGCAGCCA AAGCTCGGTC 
AGCGCAAGCA GCAGCATGAT GTCCACTACT GCCCCAAACC TGTACTCCAT TTGCACAGTC 
ACTCCCTCTC AGAGTGAAAC AAGCAGCATC AAGTCAGAGT ATGCTGACCA GTGGGGTTAC 
TACAGTGACT ATGCTGCAGT GGCAGATGAC CAGATGAAGT CTCCAGTGAC CCATTCTGCT 
AGTACCTCTT CTGCTCTCAG TGATTACAGC ATCAGCCACT TCAGTGATGG CTCAAGGGCT 
TCTGTACCAC AAGTGCCCGC TGGACTGGCC AAACCAAAGA GCACTTCTCC AGAGAAGTCC 
CACCGAGTCA CATCGCCATC CAGTGGGTAC TCCAGCCAGT CCAATACACC CACTGCACTC 
ACTCCAGTGC CTGTGTTTTT AAAATCTGCG TCATCAGGAA ATGGGAAATC CAAGCTGAAA 
CCTAAAGTGC CTGAAAGGAA ATCTTCTCTT CTGTCTTCAG TCTCCATTTC TTCATCCTCT 
ACTTCTCTTT CTTCAAATAC ATCTACTGAA GGGAGTGTGA ATGTGAAGAA ATTGGACCCC 
ACCCCGAGTT CTCCTCCAGA TTCTGGTGCA CCTCTTCCTC CGCCTCCTCT TCTAGCACCT 
CCTCCACATT GCCTTGAGCT CTCTCCTCCC TCTTCCCCTC CTCCTCCTCC AGCAGAAGCC 
GTGGATATAT CACCATTGCC AGCTTCTCCC ACATTCCCCC CCCCTCCACC AGAAGCTGAT 
GTAAATTCTT CCTTTGCTCA CAGTGTCCAG TGGTTACCAC AAGAAGCTTC CATCGGTTCG 
TACTCTTCCC CTGTTCCTCC TCCTGTTTTC CCTTTAGTTG TCCCACCACC AGCCCCACCT 
CTTGATCCCA AACTGACAAA AGGTGCAATG ATATGCCCAC AGTATTCATT TAAGAAACAT 
GATCAGGAAG ATTCTTGCTA CAATGCTGTG AAACAGCCAT TCAGCAAGCA AGATGCATCA 
AGACCTGTGA TGCCCTTGGT AACCACCAAA GCATTGCAAA TGGTGCAGCT GAGGTCTATC 
AGAAAAGCAA CAGAAGGTGA CCCATCACCT GAATCTGCTT CTGAAACCAT CTCTCAGGAA 
AAGGGTATTG TCACTTCATC ATCACAGTCT TCCCTAAGGC CATCTCTCTC ACTACGACTC 
AGTAACAGCT TAGGTGAAGA GGAGATGAAG ATTCATGGCA CTTCATTTAA AAACACACCT 
CAAACACTGG CTCAGAGTTC TCCTCTGGTA CTCTCTGACA ACGTACCCAT GCTTGAGAGC 
GATCAAAAAG CTGCAGTTAC TATGGGTCTA AGCAAGTCTT TGGAACCAGA TTCCCCAGGG 
TCAGCTCCAG AAGCCACACC CAAGTCCCCA GTTCGGAGCG AAGACTTCCA TTCTGGCATG 
CCACTGCAGG GGTCACCAGC ATCTCCAGAC AAGACTCAGG TCGTTTTGCC AAACAAGAAA 
CCACCTCCCA TTTCAAAGAA ACCCAAACTG TTCCTTGTTG TACCACCTCC ACAGTTAGAT 
CTCACAGTGG AGAAAATAGC TGAAGTGAGT GATACTGTCA GAAGCACATC AAGCCCAACT 
AAGAGAGACG CTGTGCTTAC ACACTGCGAG GAGGCGAGAA ATTGCCTTAC AGATGGACTC 
AGCTCCAGCG AGATGGACTC TGGCAGCCTG GTTCCTGAGG GAGGAGCTGC CAGAGTCACC 
TTCTCTGAAG CAGTGGGAGT TGGTGCCTTT GTGGTACAGT CCGCTGCATC ACCAGTTCAA 
GAGGAGCCCA GGCAAGAGGA GCAGTCAGTT TTTGATGAAG GAAGCAGCTC AGGCAGCAGC 
CAAGACAGCA GCAGTAACAC TGAAGGACAC CCGTCTCAGG AGAACGAAAG TGCCGAGGTG 
TTTGAATCAG AAACAGCAAA TAGTTCATTC CTGCCAAGCA ATAATTATGG GGAAGAGACG 
GATGGAGTGG CAACACCAGC GAGACCGAGG ACTACTGAGG ATCTTTTTGC AGCCATTCAC 
AGATCCAAAA GGAAAGTCCT TGGCCGTAAG GATTCTGAGG ACGATCGTAC CCGCAACCAT 
TCTCCTTCAC CCCCCGTAAC ACCCACTGGT GCTTCCCCAA CCTTATCTAC CCTCAAACAG 
GCAGGATCTA TTCAGAGAAG CGTTCGCAAG AGCAGCACCA GCAATGACAA CTTCAAAGCC 
CTGCTGCTGA AGAAAGGGAG TCGCTGTGAC ACCAGTTCCC GCATGTCAGC AGCAGAGATG 
TTGAAGCACA CGGATCCACG GTTCCACCGA GCCAAGGTGG ATGCCTCTCC CGACCTTTCC 
GACAGCCCTG CCAGCTGCTC GCCCAGCAAG AGCAAACGGG CCCAAGAGGA GTGGGCCAAG 
AGTGAAGGCC TGATGCCGAG GAGCATGTCC CTCTCCAGCA CTAGGTATGG CCGATCACGA 
ACACCGCCCT CCGCTGCCAG CAGCAAGTAC AATGTTCGCA ACCGCATCCA GAGCAGCCCC 
ATGACAGTGA TTAGCGAAGG AGATGGGGAA GTGGTGGAGC AGACAGAGAG CAGGGTCCAA 
AGGACTTTGG ATGAGCAGCG AGAGAGGCAG CTCGATGTAT TTAACAGTGA TGAAATAGAC 
ATGAATGACT TTTCGTATTC TGAGGAGCTG GACTGCAAGG AGACCTTGGA TCCAACCCAT 
CTGGACTTAA TGACTCAGCC GGGTACTTCG AGAAAGTACC TAAGTCCTTC AGCTGAAGAA 
AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_015284193.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015139679.1 Gallus gallus NHS-like 1 (NHSL1), transcript variant X3, mRNA.

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CloneID	OGa33683
Clone ID Related Accession (Same CDS sequence)	XM_015284193.2 , XM_015284193.1
Accession Version	XM_015284193.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	NHS-like protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455837.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTTCC CCCTGCGGAC CGTGGAGCCT CTGAAGCTGT GCAGGTTGGA GGAAGGCGAC 
GGCGCGGAGC GGGGCGGCCC GGCGCCGGGG GACCACGCCG GGGCGGCCGA AGGGGGCGGC 
GGGCGGCGGC GAGGCGCGGT GCCTCAGTTC GGGGCGCTGG AACAGGTCAG CTCGTACGCG 
CTGGTGTCGC TGCTCATGCA GCTCTCCGAC CTGTCCCGCT GCGCCGGAGA CATCTTCGGC 
GGGATCCTGA GCGAGGCGGA CACGCTGTGC CACCGCAACG CCCGGCTCCA GCGCCGTCTG 
GGAGCCCTCG AAGCTCTGCT CGCCCGCCTC GACCACCGCA AAGTGAAGAT CCCTGTTTCC 
AACTTAGATG AAGAGAGCAG ATGGACCGTC CATTACACTG CTCCATGGCA CCAGCAGGAA 
AATGTGTTCC TGCCTTCCTC AAGGCCGCCT TGTGTGGAGG ACTTGCACCG GCAAGCAAAG 
CTGAACCTCA AGTCAGTACT GAGAGAGTGT GACAAGCTGC GGAGAGATGG CTATCGGAGC 
TCTCAGTATT ACTCTCAGGG CCCCACCTTC TCCTCATCCT CCAGTGCAAT CTGTGGAAGT 
TACCAGGACG ATTATGAAGA AATTGAACAA AAGTGTTCTG TCTCTTCCCC AGAAGAAGAA 
AAGCTTATCA CCATCAAAAG GCCTAAAACC CCAGTTTCAA ATGACTTATC AGATATCAAC 
ACACAAACCA ACTGGACTAA GTCACTTCCA CTGCCAACAC CAGAAGAGAA GATGCGACAG 
CAAGCACAGG CAGTCCAAAC AGATGTGGTT CCTATCAATG TGACTGCAGT TGGCACAGGC 
CAAAGTGATT TCCGAGGGCA CTCGATGTAT GTCCCAGATC ACAGTTCCAC GCTAGGGCGA 
CTAGACAGCT ATCGCTCTGC TATGCAGCGG TCTGAAACCA AGGACACCAG CTGCCAGACT 
GAAGAAGTTA AAGTTGTGCC TCCTTCGATG AGAAGAATAC GAGCACAGAA AGGACAAGGC 
ATTGCAGCTC AGATGTCTCA GTTCTCCAGC TCATCTGGAA ACATGTCTGT GATGAGTGAT 
TCTGCTGCAG TTATATTTGC TTCTCGCCAG AATAGTGACA TAGGTTTTCA CAGCTTGCCT 
CGGGCTGGTG CAAGAGTGTC TCTGCAGTCC TTAGAACAGA CCCAGAGCAT CTCCAGACAG 
ACAGAAGACA TTGCTGGTAC TTTGCCTCAC CAGATGAGTA AACTGCAAGT GGATGATGAT 
GTTGCACATC TGAGAAATAA TGCCATAACA GGAACTGTTT CGAGGCCAAA GTCTCAAGAG 
GTAAGAAGTT GTGACAGTGA AAAGTCCACT AGCCCAGCAT GTGTGGTCTC TCCTCATGCC 
ACCTACTCAA CAAGCGTCAT ACCCAATGCG ACACTGTCAT CCTCCTCAGA AGTTATTGCT 
ATTCACACTG CCCAGAGTAC TGGATCGCTG GATAACAAAA TTAGCAGTTC TGCTGTGTAC 
CCAAAACCAA GAGATAATCC TGTTGCCAAC AATGCAATAA GTGGGAAAGA AGATCACCAT 
TCTTCAAGTG GTAACTGGAG TGAGAGTAGC TCCACACGTC ACTCACAGAC TTCAGATACC 
ATCCCATCTC ATACTGTCAT GATGCTTTCT CTTGGTGACT CTGCTGTCTC TCTCAGCACT 
CCTGGAAATG TAGAGGCTGG CTCTCAGAGT GTGAACTACA GCTGCAGAAG CAATCTTGCT 
TCACCAAACC AGTCCCAGGA CAGCGATGGT AGGAGTGAAT CTAGCTACTC AGGGGAGCGA 
ACACAAGGTG TAGTGAGCAG CACAGAGCAC TGGCTATACA AGTCTGCGGA AAATAGTGAG 
ACTGCATCAT GCAAGGTAGC TTGTACCACA CCAGGCTGTG CCACTCCTGG TAGCAACCTG 
AGCAGCAGCA GCCTGGAGAG GACATCAGCC AAAGAAGATT CCACTTCACT GTATTCTGTA 
GACCACGATG GTTATTACAG CTCTATGAGC ATGGACTCTG GACTGAAGTC AGACATGCCA 
TGCTATAGTA CTAATGGTTT TGGGAACCCC AGAGACAGCG TGATTAATGT CTTTGAAGCA 
AAGGAGAAGA AACATCAAGA TGACCAGCCA GGCCAAGGTG AAAAATCCCT TGCAAGAAAC 
ATCTCTCTAA AAAAGGCAAA GAAGCCGCCT TTACCTCCGT CTAGAACAGA CTCACTCAGA 
AGGGTACCCA AGAAGAAAGC TCAGTCCAAT GGACAGGTAC TTGATGAAAC CCTCATTGCT 
ACCCTCCAGC ATTCTCTGCA GTTGAATCTC AAGTGCAAAA ATGGCAGCTC CCCTTCCCAG 
AGCCCCTGCA GTGACTATGA GGATCCCTGG GTGTTGCGCT CTCGCAGCCA AAGCTCGGTC 
AGCGCAAGCA GCAGCATGAT GTCCACTACT GCCCCAAACC TGTACTCCAT TTGCACAGTC 
ACTCCCTCTC AGAGTGAAAC AAGCAGCATC AAGTCAGAGT ATGCTGACCA GTGGGGTTAC 
TACAGTGACT ATGCTGCAGT GGCAGATGAC CAGATGAAGT CTCCAGTGAC CCATTCTGCT 
AGTACCTCTT CTGCTCTCAG TGATTACAGC ATCAGCCACT TCAGTGATGG CTCAAGGGCT 
TCTGTACCAC AAGTGCCCGC TGGACTGGCC AAACCAAAGA GCACTTCTCC AGAGAAGTCC 
CACCGAGTCA CATCGCCATC CAGTGGGTAC TCCAGCCAGT CCAATACACC CACTGCACTC 
ACTCCAGTGC CTGTGTTTTT AAAATCTGCG TCATCAGGAA ATGGGAAATC CAAGCTGAAA 
CCTAAAGTGC CTGAAAGGAA ATCTTCTCTT CTGTCTTCAG TCTCCATTTC TTCATCCTCT 
ACTTCTCTTT CTTCAAATAC ATCTACTGAA GGGAGTGTGA ATGTGAAGAA ATTGGACCCC 
ACCCCGAGTT CTCCTCCAGA TTCTGGTGCA CCTCTTCCTC CGCCTCCTCT TCTAGCACCT 
CCTCCACATT GCCTTGAGCT CTCTCCTCCC TCTTCCCCTC CTCCTCCTCC AGCAGAAGCC 
GTGGATATAT CACCATTGCC AGCTTCTCCC ACATTCCCCC CCCCTCCACC AGAAGCTGAT 
GTAAATTCTT CCTTTGCTCA CAGTGTCCAG TGGTTACCAC AAGAAGCTTC CATCGGTTCG 
TACTCTTCCC CTGTTCCTCC TCCTGTTTTC CCTTTAGTTG TCCCACCACC AGCCCCACCT 
CTTGATCCCA AACTGACAAA AGGTGCAATG ATATGCCCAC AGTATTCATT TAAGAAACAT 
GATCAGGAAG ATTCTTGCTA CAATGCTGTG AAACAGCCAT TCAGCAAGCA AGATGCATCA 
AGACCTGTGA TGCCCTTGGT AACCACCAAA GCATTGCAAA TGGTGCAGCT GAGGTCTATC 
AGAAAAGCAA CAGAAGGTGA CCCATCACCT GAATCTGCTT CTGAAACCAT CTCTCAGGAA 
AAGGGTATTG TCACTTCATC ATCACAGTCT TCCCTAAGGC CATCTCTCTC ACTACGACTC 
AGTAACAGCT TAGGTGAAGA GGAGATGAAG ATTCATGGCA CTTCATTTAA AAACACACCT 
CAAACACTGG CTCAGAGTTC TCCTCTGGTA CTCTCTGACA ACGTACCCAT GCTTGAGAGC 
GATCAAAAAG CTGCAGTTAC TATGGGTCTA AGCAAGTCTT TGGAACCAGA TTCCCCAGGG 
TCAGCTCCAG AAGCCACACC CAAGTCCCCA GTTCGGAGCG AAGACTTCCA TTCTGGCATG 
CCACTGCAGG GGTCACCAGC ATCTCCAGAC AAGACTCAGG TCGTTTTGCC AAACAAGAAA 
CCACCTCCCA TTTCAAAGAA ACCCAAACTG TTCCTTGTTG TACCACCTCC ACAGTTAGAT 
CTCACAGTGG AGAAAATAGC TGAAGTGAGT GATACTGTCA GAAGCACATC AAGCCCAACT 
AAGAGAGACG CTGTGCTTAC ACACTGCGAG GAGGCGAGAA ATTGCCTTAC AGATGGACTC 
AGCTCCAGCG AGATGGACTC TGGCAGCCTG GTTCCTGAGG GAGGAGCTGC CAGAGTCACC 
TTCTCTGAAG CAGTGGGAGT TGGTGCCTTT GTGGTACAGT CCGCTGCATC ACCAGTTCAA 
GAGGAGCCCA GGCAAGAGGA GCAGTCAGTT TTTGATGAAG GAAGCAGCTC AGGCAGCAGC 
CAAGACAGCA GCAGTAACAC TGAAGGACAC CCGTCTCAGG AGAACGAAAG TGCCGAGGTG 
TTTGAATCAG AAACAGCAAA TAGTTCATTC CTGCCAAGCA ATAATTATGG GGAAGAGACG 
GATGGAGTGG CAACACCAGC GAGACCGAGG ACTACTGAGG ATCTTTTTGC AGCCATTCAC 
AGATCCAAAA GGAAAGTCCT TGGCCGTAAG GATTCTGAGG ACGATCGTAC CCGCAACCAT 
TCTCCTTCAC CCCCCGTAAC ACCCACTGGT GCTTCCCCAA CCTTATCTAC CCTCAAACAG 
GCAGGATCTA TTCAGAGAAG CGTTCGCAAG AGCAGCACCA GCAATGACAA CTTCAAAGCC 
CTGCTGCTGA AGAAAGGGAG TCGCTGTGAC ACCAGTTCCC GCATGTCAGC AGCAGAGATG 
TTGAAGCACA CGGATCCACG GTTCCACCGA GCCAAGGTGG ATGCCTCTCC CGACCTTTCC 
GACAGCCCTG CCAGCTGCTC GCCCAGCAAG AGCAAACGGG CCCAAGAGGA GTGGGCCAAG 
AGTGAAGGCC TGATGCCGAG GAGCATGTCC CTCTCCAGCA CTAGGTATGG CCGATCACGA 
ACACCGCCCT CCGCTGCCAG CAGCAAGTAC AATGTTCGCA ACCGCATCCA GAGCAGCCCC 
ATGACAGTGA TTAGCGAAGG AGATGGGGAA GTGGTGGAGC AGACAGAGAG CAGGGTCCAA 
AGGACTTTGG ATGAGCAGCG AGAGAGGCAG CTCGATGTAT TTAACAGTGA TGAAATAGAC 
ATGAATGACT TTTCGTATTC TGAGGAGCTG GACTGCAAGG AGACCTTGGA TCCAACCCAT 
CTGGACTTAA TGACTCAGCC GGGTACTTCG AGAAAGTACC TAAGTCCTTC AGCTGAAGAA 
AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015139679.1
CDS	94..5199
Translation	MPFPLRTVEPLKLCRLEEGDGAERGGPAPGDHAGAAEGGGGRRRGAVPQFGALEQVSSYALVSLLMQLSDLSRCAGDIFGGILSEADTLCHRNARLQRRLGALEALLARLDHRKVKIPVSNLDEESRWTVHYTAPWHQQENVFLPSSRPPCVEDLHRQAKLNLKSVLRECDKLRRDGYRSSQYYSQGPTFSSSSSAICGSYQDDYEEIEQKCSVSSPEEEKLITIKRPKTPVSNDLSDINTQTNWTKSLPLPTPEEKMRQQAQAVQTDVVPINVTAVGTGQSDFRGHSMYVPDHSSTLGRLDSYRSAMQRSETKDTSCQTEEVKVVPPSMRRIRAQKGQGIAAQMSQFSSSSGNMSVMSDSAAVIFASRQNSDIGFHSLPRAGARVSLQSLEQTQSISRQTEDIAGTLPHQMSKLQVDDDVAHLRNNAITGTVSRPKSQEVRSCDSEKSTSPACVVSPHATYSTSVIPNATLSSSSEVIAIHTAQSTGSLDNKISSSAVYPKPRDNPVANNAISGKEDHHSSSGNWSESSSTRHSQTSDTIPSHTVMMLSLGDSAVSLSTPGNVEAGSQSVNYSCRSNLASPNQSQDSDGRSESSYSGERTQGVVSSTEHWLYKSAENSETASCKVACTTPGCATPGSNLSSSSLERTSAKEDSTSLYSVDHDGYYSSMSMDSGLKSDMPCYSTNGFGNPRDSVINVFEAKEKKHQDDQPGQGEKSLARNISLKKAKKPPLPPSRTDSLRRVPKKKAQSNGQVLDETLIATLQHSLQLNLKCKNGSSPSQSPCSDYEDPWVLRSRSQSSVSASSSMMSTTAPNLYSICTVTPSQSETSSIKSEYADQWGYYSDYAAVADDQMKSPVTHSASTSSALSDYSISHFSDGSRASVPQVPAGLAKPKSTSPEKSHRVTSPSSGYSSQSNTPTALTPVPVFLKSASSGNGKSKLKPKVPERKSSLLSSVSISSSSTSLSSNTSTEGSVNVKKLDPTPSSPPDSGAPLPPPPLLAPPPHCLELSPPSSPPPPPAEAVDISPLPASPTFPPPPPEADVNSSFAHSVQWLPQEASIGSYSSPVPPPVFPLVVPPPAPPLDPKLTKGAMICPQYSFKKHDQEDSCYNAVKQPFSKQDASRPVMPLVTTKALQMVQLRSIRKATEGDPSPESASETISQEKGIVTSSSQSSLRPSLSLRLSNSLGEEEMKIHGTSFKNTPQTLAQSSPLVLSDNVPMLESDQKAAVTMGLSKSLEPDSPGSAPEATPKSPVRSEDFHSGMPLQGSPASPDKTQVVLPNKKPPPISKKPKLFLVVPPPQLDLTVEKIAEVSDTVRSTSSPTKRDAVLTHCEEARNCLTDGLSSSEMDSGSLVPEGGAARVTFSEAVGVGAFVVQSAASPVQEEPRQEEQSVFDEGSSSGSSQDSSSNTEGHPSQENESAEVFESETANSSFLPSNNYGEETDGVATPARPRTTEDLFAAIHRSKRKVLGRKDSEDDRTRNHSPSPPVTPTGASPTLSTLKQAGSIQRSVRKSSTSNDNFKALLLKKGSRCDTSSRMSAAEMLKHTDPRFHRAKVDASPDLSDSPASCSPSKSKRAQEEWAKSEGLMPRSMSLSSTRYGRSRTPPSAASSKYNVRNRIQSSPMTVISEGDGEVVEQTESRVQRTLDEQRERQLDVFNSDEIDMNDFSYSEELDCKETLDPTHLDLMTQPGTSRKYLSPSAEES

Target ORF information:

RefSeq Version	XM_015284193.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NHS-like 1 (NHSL1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015284193.1

ORF Insert Sequence:

ATGCCGTTCC CCCTGCGGAC CGTGGAGCCT CTGAAGCTGT GCAGGTTGGA GGAAGGCGAC 
GGCGCGGAGC GGGGCGGCCC GGCGCCGGGG GACCACGCCG GGGCGGCCGA AGGGGGCGGC 
GGGCGGCGGC GAGGCGCGGT GCCTCAGTTC GGGGCGCTGG AACAGGTCAG CTCGTACGCG 
CTGGTGTCGC TGCTCATGCA GCTCTCCGAC CTGTCCCGCT GCGCCGGAGA CATCTTCGGC 
GGGATCCTGA GCGAGGCGGA CACGCTGTGC CACCGCAACG CCCGGCTCCA GCGCCGTCTG 
GGAGCCCTCG AAGCTCTGCT CGCCCGCCTC GACCACCGCA AAGTGAAGAT CCCTGTTTCC 
AACTTAGATG AAGAGAGCAG ATGGACCGTC CATTACACTG CTCCATGGCA CCAGCAGGAA 
AATGTGTTCC TGCCTTCCTC AAGGCCGCCT TGTGTGGAGG ACTTGCACCG GCAAGCAAAG 
CTGAACCTCA AGTCAGTACT GAGAGAGTGT GACAAGCTGC GGAGAGATGG CTATCGGAGC 
TCTCAGTATT ACTCTCAGGG CCCCACCTTC TCCTCATCCT CCAGTGCAAT CTGTGGAAGT 
TACCAGGACG ATTATGAAGA AATTGAACAA AAGTGTTCTG TCTCTTCCCC AGAAGAAGAA 
AAGCTTATCA CCATCAAAAG GCCTAAAACC CCAGTTTCAA ATGACTTATC AGATATCAAC 
ACACAAACCA ACTGGACTAA GTCACTTCCA CTGCCAACAC CAGAAGAGAA GATGCGACAG 
CAAGCACAGG CAGTCCAAAC AGATGTGGTT CCTATCAATG TGACTGCAGT TGGCACAGGC 
CAAAGTGATT TCCGAGGGCA CTCGATGTAT GTCCCAGATC ACAGTTCCAC GCTAGGGCGA 
CTAGACAGCT ATCGCTCTGC TATGCAGCGG TCTGAAACCA AGGACACCAG CTGCCAGACT 
GAAGAAGTTA AAGTTGTGCC TCCTTCGATG AGAAGAATAC GAGCACAGAA AGGACAAGGC 
ATTGCAGCTC AGATGTCTCA GTTCTCCAGC TCATCTGGAA ACATGTCTGT GATGAGTGAT 
TCTGCTGCAG TTATATTTGC TTCTCGCCAG AATAGTGACA TAGGTTTTCA CAGCTTGCCT 
CGGGCTGGTG CAAGAGTGTC TCTGCAGTCC TTAGAACAGA CCCAGAGCAT CTCCAGACAG 
ACAGAAGACA TTGCTGGTAC TTTGCCTCAC CAGATGAGTA AACTGCAAGT GGATGATGAT 
GTTGCACATC TGAGAAATAA TGCCATAACA GGAACTGTTT CGAGGCCAAA GTCTCAAGAG 
GTAAGAAGTT GTGACAGTGA AAAGTCCACT AGCCCAGCAT GTGTGGTCTC TCCTCATGCC 
ACCTACTCAA CAAGCGTCAT ACCCAATGCG ACACTGTCAT CCTCCTCAGA AGTTATTGCT 
ATTCACACTG CCCAGAGTAC TGGATCGCTG GATAACAAAA TTAGCAGTTC TGCTGTGTAC 
CCAAAACCAA GAGATAATCC TGTTGCCAAC AATGCAATAA GTGGGAAAGA AGATCACCAT 
TCTTCAAGTG GTAACTGGAG TGAGAGTAGC TCCACACGTC ACTCACAGAC TTCAGATACC 
ATCCCATCTC ATACTGTCAT GATGCTTTCT CTTGGTGACT CTGCTGTCTC TCTCAGCACT 
CCTGGAAATG TAGAGGCTGG CTCTCAGAGT GTGAACTACA GCTGCAGAAG CAATCTTGCT 
TCACCAAACC AGTCCCAGGA CAGCGATGGT AGGAGTGAAT CTAGCTACTC AGGGGAGCGA 
ACACAAGGTG TAGTGAGCAG CACAGAGCAC TGGCTATACA AGTCTGCGGA AAATAGTGAG 
ACTGCATCAT GCAAGGTAGC TTGTACCACA CCAGGCTGTG CCACTCCTGG TAGCAACCTG 
AGCAGCAGCA GCCTGGAGAG GACATCAGCC AAAGAAGATT CCACTTCACT GTATTCTGTA 
GACCACGATG GTTATTACAG CTCTATGAGC ATGGACTCTG GACTGAAGTC AGACATGCCA 
TGCTATAGTA CTAATGGTTT TGGGAACCCC AGAGACAGCG TGATTAATGT CTTTGAAGCA 
AAGGAGAAGA AACATCAAGA TGACCAGCCA GGCCAAGGTG AAAAATCCCT TGCAAGAAAC 
ATCTCTCTAA AAAAGGCAAA GAAGCCGCCT TTACCTCCGT CTAGAACAGA CTCACTCAGA 
AGGGTACCCA AGAAGAAAGC TCAGTCCAAT GGACAGGTAC TTGATGAAAC CCTCATTGCT 
ACCCTCCAGC ATTCTCTGCA GTTGAATCTC AAGTGCAAAA ATGGCAGCTC CCCTTCCCAG 
AGCCCCTGCA GTGACTATGA GGATCCCTGG GTGTTGCGCT CTCGCAGCCA AAGCTCGGTC 
AGCGCAAGCA GCAGCATGAT GTCCACTACT GCCCCAAACC TGTACTCCAT TTGCACAGTC 
ACTCCCTCTC AGAGTGAAAC AAGCAGCATC AAGTCAGAGT ATGCTGACCA GTGGGGTTAC 
TACAGTGACT ATGCTGCAGT GGCAGATGAC CAGATGAAGT CTCCAGTGAC CCATTCTGCT 
AGTACCTCTT CTGCTCTCAG TGATTACAGC ATCAGCCACT TCAGTGATGG CTCAAGGGCT 
TCTGTACCAC AAGTGCCCGC TGGACTGGCC AAACCAAAGA GCACTTCTCC AGAGAAGTCC 
CACCGAGTCA CATCGCCATC CAGTGGGTAC TCCAGCCAGT CCAATACACC CACTGCACTC 
ACTCCAGTGC CTGTGTTTTT AAAATCTGCG TCATCAGGAA ATGGGAAATC CAAGCTGAAA 
CCTAAAGTGC CTGAAAGGAA ATCTTCTCTT CTGTCTTCAG TCTCCATTTC TTCATCCTCT 
ACTTCTCTTT CTTCAAATAC ATCTACTGAA GGGAGTGTGA ATGTGAAGAA ATTGGACCCC 
ACCCCGAGTT CTCCTCCAGA TTCTGGTGCA CCTCTTCCTC CGCCTCCTCT TCTAGCACCT 
CCTCCACATT GCCTTGAGCT CTCTCCTCCC TCTTCCCCTC CTCCTCCTCC AGCAGAAGCC 
GTGGATATAT CACCATTGCC AGCTTCTCCC ACATTCCCCC CCCCTCCACC AGAAGCTGAT 
GTAAATTCTT CCTTTGCTCA CAGTGTCCAG TGGTTACCAC AAGAAGCTTC CATCGGTTCG 
TACTCTTCCC CTGTTCCTCC TCCTGTTTTC CCTTTAGTTG TCCCACCACC AGCCCCACCT 
CTTGATCCCA AACTGACAAA AGGTGCAATG ATATGCCCAC AGTATTCATT TAAGAAACAT 
GATCAGGAAG ATTCTTGCTA CAATGCTGTG AAACAGCCAT TCAGCAAGCA AGATGCATCA 
AGACCTGTGA TGCCCTTGGT AACCACCAAA GCATTGCAAA TGGTGCAGCT GAGGTCTATC 
AGAAAAGCAA CAGAAGGTGA CCCATCACCT GAATCTGCTT CTGAAACCAT CTCTCAGGAA 
AAGGGTATTG TCACTTCATC ATCACAGTCT TCCCTAAGGC CATCTCTCTC ACTACGACTC 
AGTAACAGCT TAGGTGAAGA GGAGATGAAG ATTCATGGCA CTTCATTTAA AAACACACCT 
CAAACACTGG CTCAGAGTTC TCCTCTGGTA CTCTCTGACA ACGTACCCAT GCTTGAGAGC 
GATCAAAAAG CTGCAGTTAC TATGGGTCTA AGCAAGTCTT TGGAACCAGA TTCCCCAGGG 
TCAGCTCCAG AAGCCACACC CAAGTCCCCA GTTCGGAGCG AAGACTTCCA TTCTGGCATG 
CCACTGCAGG GGTCACCAGC ATCTCCAGAC AAGACTCAGG TCGTTTTGCC AAACAAGAAA 
CCACCTCCCA TTTCAAAGAA ACCCAAACTG TTCCTTGTTG TACCACCTCC ACAGTTAGAT 
CTCACAGTGG AGAAAATAGC TGAAGTGAGT GATACTGTCA GAAGCACATC AAGCCCAACT 
AAGAGAGACG CTGTGCTTAC ACACTGCGAG GAGGCGAGAA ATTGCCTTAC AGATGGACTC 
AGCTCCAGCG AGATGGACTC TGGCAGCCTG GTTCCTGAGG GAGGAGCTGC CAGAGTCACC 
TTCTCTGAAG CAGTGGGAGT TGGTGCCTTT GTGGTACAGT CCGCTGCATC ACCAGTTCAA 
GAGGAGCCCA GGCAAGAGGA GCAGTCAGTT TTTGATGAAG GAAGCAGCTC AGGCAGCAGC 
CAAGACAGCA GCAGTAACAC TGAAGGACAC CCGTCTCAGG AGAACGAAAG TGCCGAGGTG 
TTTGAATCAG AAACAGCAAA TAGTTCATTC CTGCCAAGCA ATAATTATGG GGAAGAGACG 
GATGGAGTGG CAACACCAGC GAGACCGAGG ACTACTGAGG ATCTTTTTGC AGCCATTCAC 
AGATCCAAAA GGAAAGTCCT TGGCCGTAAG GATTCTGAGG ACGATCGTAC CCGCAACCAT 
TCTCCTTCAC CCCCCGTAAC ACCCACTGGT GCTTCCCCAA CCTTATCTAC CCTCAAACAG 
GCAGGATCTA TTCAGAGAAG CGTTCGCAAG AGCAGCACCA GCAATGACAA CTTCAAAGCC 
CTGCTGCTGA AGAAAGGGAG TCGCTGTGAC ACCAGTTCCC GCATGTCAGC AGCAGAGATG 
TTGAAGCACA CGGATCCACG GTTCCACCGA GCCAAGGTGG ATGCCTCTCC CGACCTTTCC 
GACAGCCCTG CCAGCTGCTC GCCCAGCAAG AGCAAACGGG CCCAAGAGGA GTGGGCCAAG 
AGTGAAGGCC TGATGCCGAG GAGCATGTCC CTCTCCAGCA CTAGGTATGG CCGATCACGA 
ACACCGCCCT CCGCTGCCAG CAGCAAGTAC AATGTTCGCA ACCGCATCCA GAGCAGCCCC 
ATGACAGTGA TTAGCGAAGG AGATGGGGAA GTGGTGGAGC AGACAGAGAG CAGGGTCCAA 
AGGACTTTGG ATGAGCAGCG AGAGAGGCAG CTCGATGTAT TTAACAGTGA TGAAATAGAC 
ATGAATGACT TTTCGTATTC TGAGGAGCTG GACTGCAAGG AGACCTTGGA TCCAACCCAT 
CTGGACTTAA TGACTCAGCC GGGTACTTCG AGAAAGTACC TAAGTCCTTC AGCTGAAGAA 
AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_015284194.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015139680.1 Gallus gallus NHS-like 1 (NHSL1), transcript variant X4, mRNA.

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CloneID	OGa33684
Clone ID Related Accession (Same CDS sequence)	XM_015284194.1 , XM_015284194.2
Accession Version	XM_015284194.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	NHS-like protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455837.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTTCC CCCTGCGGAC CGTGGAGCCT CTGAAGCTGT GCAGGTTGGA GGAAGGCGAC 
GGCGCGGAGC GGGGCGGCCC GGCGCCGGGG GACCACGCCG GGGCGGCCGA AGGGGGCGGC 
GGGCGGCGGC GAGGCGCGGT GCCTCAGTTC GGGGCGCTGG AACAGGTCAG CTCGTACGCG 
CTGGTGTCGC TGCTCATGCA GCTCTCCGAC CTGTCCCGCT GCGCCGGAGA CATCTTCGGC 
GGGATCCTGA GCGAGGCGGA CACGCTGTGC CACCGCAACG CCCGGCTCCA GCGCCGTCTG 
GGAGCCCTCG AAGCTCTGCT CGCCCGCCTC GACCACCGCA AAGTGAAGAT CCCTGTTTCC 
AACTTAGATG AAGAGAGCAG ATGGACCGTC CATTACACTG CTCCATGGCA CCAGCAGGAA 
AATGTGTTCC TGCCTTCCTC AAGGCCGCCT TGTGTGGAGG ACTTGCACCG GCAAGCAAAG 
CTGAACCTCA AGTCAGTACT GAGAGAGTGT GACAAGCTGC GGAGAGATGG CTATCGGAGC 
TCTCAGTATT ACTCTCAGGG CCCCACCTTC TCCTCATCCT CCAGTGCAAT CTGTGGAAGT 
TACCAGGACG ATTATGAAGA AATTGAACAA AAGTGTTCTG TCTCTTCCCC AGAAGAAGAA 
AAGCTTATCA CCATCAAAAG GCCTAAAACC CCAGTTTCAA ATGACTTATC AGATATCAAC 
ACACAAACCA ACTGGACTAA GTCACTTCCA CTGCCAACAC CAGAAGAGAA GATGCGACAG 
CAAGCACAGG CAGTCCAAAC AGATGTGGTT CCTATCAATG TGACTGTTGG CACAGGCCAA 
AGTGATTTCC GAGGGCACTC GATGTATGTC CCAGATCACA GTTCCACGCT AGGGCGACTA 
GACAGCTATC GCTCTGCTAT GCAGCGGTCT GAAACCAAGG ACACCAGCTG CCAGACTGAA 
GAAGTTAAAG TTGTGCCTCC TTCGATGAGA AGAATACGAG CACAGAAAGG ACAAGGCATT 
GCAGCTCAGA TGTCTCAGTT CTCCAGCTCA TCTGGAAACA TGTCTGTGAT GAGTGATTCT 
GCTGCAGTTA TATTTGCTTC TCGCCAGAAT AGTGACATAG GTTTTCACAG CTTGCCTCGG 
GCTGGTGCAA GAGTGTCTCT GCAGTCCTTA GAACAGACCC AGAGCATCTC CAGACAGACA 
GAAGACATTG CTGGTACTTT GCCTCACCAG ATGAGTAAAC TGCAAGTGGA TGATGATGTT 
GCACATCTGA GAAATAATGC CATAACAGGA ACTGTTTCGA GGCCAAAGTC TCAAGAGGTA 
AGAAGTTGTG ACAGTGAAAA GTCCACTAGC CCAGCATGTG TGGTCTCTCC TCATGCCACC 
TACTCAACAA GCGTCATACC CAATGCGACA CTGTCATCCT CCTCAGAAGT TATTGCTATT 
CACACTGCCC AGAGTACTGG ATCGCTGGAT AACAAAATTA GCAGTTCTGC TGTGTACCCA 
AAACCAAGAG ATAATCCTGT TGCCAACAAT GCAATAAGTG GGAAAGAAGA TCACCATTCT 
TCAAGTGGTA ACTGGAGTGA GAGTAGCTCC ACACGTCACT CACAGACTTC AGATACCATC 
CCATCTCATA CTGTCATGAT GCTTTCTCTT GGTGACTCTG CTGTCTCTCT CAGCACTCCT 
GGAAATGTAG AGGCTGGCTC TCAGAGTGTG AACTACAGCT GCAGAAGCAA TCTTGCTTCA 
CCAAACCAGT CCCAGGACAG CGATGGTAGG AGTGAATCTA GCTACTCAGG GGAGCGAACA 
CAAGGTGTAG TGAGCAGCAC AGAGCACTGG CTATACAAGT CTGCGGAAAA TAGTGAGACT 
GCATCATGCA AGGTAGCTTG TACCACACCA GGCTGTGCCA CTCCTGGTAG CAACCTGAGC 
AGCAGCAGCC TGGAGAGGAC ATCAGCCAAA GAAGATTCCA CTTCACTGTA TTCTGTAGAC 
CACGATGGTT ATTACAGCTC TATGAGCATG GACTCTGGAC TGAAGTCAGA CATGCCATGC 
TATAGTACTA ATGGTTTTGG GAACCCCAGA GACAGCGTGA TTAATGTCTT TGAAGCAAAG 
GAGAAGAAAC ATCAAGATGA CCAGCCAGGC CAAGGTGAAA AATCCCTTGC AAGAAACATC 
TCTCTAAAAA AGGCAAAGAA GCCGCCTTTA CCTCCGTCTA GAACAGACTC ACTCAGAAGG 
GTACCCAAGA AGAAAGCTCA GTCCAATGGA CAGGTACTTG ATGAAACCCT CATTGCTACC 
CTCCAGCATT CTCTGCAGTT GAATCTCAAG TGCAAAAATG GCAGCTCCCC TTCCCAGAGC 
CCCTGCAGTG ACTATGAGGA TCCCTGGGTG TTGCGCTCTC GCAGCCAAAG CTCGGTCAGC 
GCAAGCAGCA GCATGATGTC CACTACTGCC CCAAACCTGT ACTCCATTTG CACAGTCACT 
CCCTCTCAGA GTGAAACAAG CAGCATCAAG TCAGAGTATG CTGACCAGTG GGGTTACTAC 
AGTGACTATG CTGCAGTGGC AGATGACCAG ATGAAGTCTC CAGTGACCCA TTCTGCTAGT 
ACCTCTTCTG CTCTCAGTGA TTACAGCATC AGCCACTTCA GTGATGGCTC AAGGGCTTCT 
GTACCACAAG TGCCCGCTGG ACTGGCCAAA CCAAAGAGCA CTTCTCCAGA GAAGTCCCAC 
CGAGTCACAT CGCCATCCAG TGGGTACTCC AGCCAGTCCA ATACACCCAC TGCACTCACT 
CCAGTGCCTG TGTTTTTAAA ATCTGCGTCA TCAGGAAATG GGAAATCCAA GCTGAAACCT 
AAAGTGCCTG AAAGGAAATC TTCTCTTCTG TCTTCAGTCT CCATTTCTTC ATCCTCTACT 
TCTCTTTCTT CAAATACATC TACTGAAGGG AGTGTGAATG TGAAGAAATT GGACCCCACC 
CCGAGTTCTC CTCCAGATTC TGGTGCACCT CTTCCTCCGC CTCCTCTTCT AGCACCTCCT 
CCACATTGCC TTGAGCTCTC TCCTCCCTCT TCCCCTCCTC CTCCTCCAGC AGAAGCCGTG 
GATATATCAC CATTGCCAGC TTCTCCCACA TTCCCCCCCC CTCCACCAGA AGCTGATGTA 
AATTCTTCCT TTGCTCACAG TGTCCAGTGG TTACCACAAG AAGCTTCCAT CGGTTCGTAC 
TCTTCCCCTG TTCCTCCTCC TGTTTTCCCT TTAGTTGTCC CACCACCAGC CCCACCTCTT 
GATCCCAAAC TGACAAAAGG TGCAATGATA TGCCCACAGT ATTCATTTAA GAAACATGAT 
CAGGAAGATT CTTGCTACAA TGCTGTGAAA CAGCCATTCA GCAAGCAAGA TGCATCAAGA 
CCTGTGATGC CCTTGGTAAC CACCAAAGCA TTGCAAATGG TGCAGCTGAG GTCTATCAGA 
AAAGCAACAG AAGGTGACCC ATCACCTGAA TCTGCTTCTG AAACCATCTC TCAGGAAAAG 
GGTATTGTCA CTTCATCATC ACAGTCTTCC CTAAGGCCAT CTCTCTCACT ACGACTCAGT 
AACAGCTTAG GTGAAGAGGA GATGAAGATT CATGGCACTT CATTTAAAAA CACACCTCAA 
ACACTGGCTC AGAGTTCTCC TCTGGTACTC TCTGACAACG TACCCATGCT TGAGAGCGAT 
CAAAAAGCTG CAGTTACTAT GGGTCTAAGC AAGTCTTTGG AACCAGATTC CCCAGGGTCA 
GCTCCAGAAG CCACACCCAA GTCCCCAGTT CGGAGCGAAG ACTTCCATTC TGGCATGCCA 
CTGCAGGGGT CACCAGCATC TCCAGACAAG ACTCAGGTCG TTTTGCCAAA CAAGAAACCA 
CCTCCCATTT CAAAGAAACC CAAACTGTTC CTTGTTGTAC CACCTCCACA GTTAGATCTC 
ACAGTGGAGA AAATAGCTGA AGTGAGTGAT ACTGTCAGAA GCACATCAAG CCCAACTAAG 
AGAGACGCTG TGCTTACACA CTGCGAGGAG GCGAGAAATT GCCTTACAGA TGGACTCAGC 
TCCAGCGAGA TGGACTCTGG CAGCCTGGTT CCTGAGGGAG GAGCTGCCAG AGTCACCTTC 
TCTGAAGCAG TGGGAGTTGG TGCCTTTGTG GTACAGTCCG CTGCATCACC AGTTCAAGAG 
GAGCCCAGGC AAGAGGAGCA GTCAGTTTTT GATGAAGGAA GCAGCTCAGG CAGCAGCCAA 
GACAGCAGCA GTAACACTGA AGGACACCCG TCTCAGGAGA ACGAAAGTGC CGAGGTGTTT 
GAATCAGAAA CAGCAAATAG TTCATTCCTG CCAAGCAATA ATTATGGGGA AGAGACGGAT 
GGAGTGGCAA CACCAGCGAG ACCGAGGACT ACTGAGGATC TTTTTGCAGC CATTCACAGA 
TCCAAAAGGA AAGTCCTTGG CCGTAAGGAT TCTGAGGACG ATCGTACCCG CAACCATTCT 
CCTTCACCCC CCGTAACACC CACTGGTGCT TCCCCAACCT TATCTACCCT CAAACAGGCA 
GGATCTATTC AGAGAAGCGT TCGCAAGAGC AGCACCAGCA ATGACAACTT CAAAGCCCTG 
CTGCTGAAGA AAGGGAGTCG CTGTGACACC AGTTCCCGCA TGTCAGCAGC AGAGATGTTG 
AAGCACACGG ATCCACGGTT CCACCGAGCC AAGGTGGATG CCTCTCCCGA CCTTTCCGAC 
AGCCCTGCCA GCTGCTCGCC CAGCAAGAGC AAACGGGCCC AAGAGGAGTG GGCCAAGAGT 
GAAGGCCTGA TGCCGAGGAG CATGTCCCTC TCCAGCACTA GGTATGGCCG ATCACGAACA 
CCGCCCTCCG CTGCCAGCAG CAAGTACAAT GTTCGCAACC GCATCCAGAG CAGCCCCATG 
ACAGTGATTA GCGAAGGAGA TGGGGAAGTG GTGGAGCAGA CAGAGAGCAG GGTCCAAAGG 
ACTTTGGATG AGCAGCGAGA GAGGCAGCTC GATGTATTTA ACAGTGATGA AATAGACATG 
AATGACTTTT CGTATTCTGA GGAGCTGGAC TGCAAGGAGA CCTTGGATCC AACCCATCTG 
GACTTAATGA CTCAGCCGGG TACTTCGAGA AAGTACCTAA GTCCTTCAGC TGAAGAAAGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015139680.1
CDS	94..5196
Translation	MPFPLRTVEPLKLCRLEEGDGAERGGPAPGDHAGAAEGGGGRRRGAVPQFGALEQVSSYALVSLLMQLSDLSRCAGDIFGGILSEADTLCHRNARLQRRLGALEALLARLDHRKVKIPVSNLDEESRWTVHYTAPWHQQENVFLPSSRPPCVEDLHRQAKLNLKSVLRECDKLRRDGYRSSQYYSQGPTFSSSSSAICGSYQDDYEEIEQKCSVSSPEEEKLITIKRPKTPVSNDLSDINTQTNWTKSLPLPTPEEKMRQQAQAVQTDVVPINVTVGTGQSDFRGHSMYVPDHSSTLGRLDSYRSAMQRSETKDTSCQTEEVKVVPPSMRRIRAQKGQGIAAQMSQFSSSSGNMSVMSDSAAVIFASRQNSDIGFHSLPRAGARVSLQSLEQTQSISRQTEDIAGTLPHQMSKLQVDDDVAHLRNNAITGTVSRPKSQEVRSCDSEKSTSPACVVSPHATYSTSVIPNATLSSSSEVIAIHTAQSTGSLDNKISSSAVYPKPRDNPVANNAISGKEDHHSSSGNWSESSSTRHSQTSDTIPSHTVMMLSLGDSAVSLSTPGNVEAGSQSVNYSCRSNLASPNQSQDSDGRSESSYSGERTQGVVSSTEHWLYKSAENSETASCKVACTTPGCATPGSNLSSSSLERTSAKEDSTSLYSVDHDGYYSSMSMDSGLKSDMPCYSTNGFGNPRDSVINVFEAKEKKHQDDQPGQGEKSLARNISLKKAKKPPLPPSRTDSLRRVPKKKAQSNGQVLDETLIATLQHSLQLNLKCKNGSSPSQSPCSDYEDPWVLRSRSQSSVSASSSMMSTTAPNLYSICTVTPSQSETSSIKSEYADQWGYYSDYAAVADDQMKSPVTHSASTSSALSDYSISHFSDGSRASVPQVPAGLAKPKSTSPEKSHRVTSPSSGYSSQSNTPTALTPVPVFLKSASSGNGKSKLKPKVPERKSSLLSSVSISSSSTSLSSNTSTEGSVNVKKLDPTPSSPPDSGAPLPPPPLLAPPPHCLELSPPSSPPPPPAEAVDISPLPASPTFPPPPPEADVNSSFAHSVQWLPQEASIGSYSSPVPPPVFPLVVPPPAPPLDPKLTKGAMICPQYSFKKHDQEDSCYNAVKQPFSKQDASRPVMPLVTTKALQMVQLRSIRKATEGDPSPESASETISQEKGIVTSSSQSSLRPSLSLRLSNSLGEEEMKIHGTSFKNTPQTLAQSSPLVLSDNVPMLESDQKAAVTMGLSKSLEPDSPGSAPEATPKSPVRSEDFHSGMPLQGSPASPDKTQVVLPNKKPPPISKKPKLFLVVPPPQLDLTVEKIAEVSDTVRSTSSPTKRDAVLTHCEEARNCLTDGLSSSEMDSGSLVPEGGAARVTFSEAVGVGAFVVQSAASPVQEEPRQEEQSVFDEGSSSGSSQDSSSNTEGHPSQENESAEVFESETANSSFLPSNNYGEETDGVATPARPRTTEDLFAAIHRSKRKVLGRKDSEDDRTRNHSPSPPVTPTGASPTLSTLKQAGSIQRSVRKSSTSNDNFKALLLKKGSRCDTSSRMSAAEMLKHTDPRFHRAKVDASPDLSDSPASCSPSKSKRAQEEWAKSEGLMPRSMSLSSTRYGRSRTPPSAASSKYNVRNRIQSSPMTVISEGDGEVVEQTESRVQRTLDEQRERQLDVFNSDEIDMNDFSYSEELDCKETLDPTHLDLMTQPGTSRKYLSPSAEES

Target ORF information:

RefSeq Version	XM_015284194.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NHS-like 1 (NHSL1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015284194.1

ORF Insert Sequence:

ATGCCGTTCC CCCTGCGGAC CGTGGAGCCT CTGAAGCTGT GCAGGTTGGA GGAAGGCGAC 
GGCGCGGAGC GGGGCGGCCC GGCGCCGGGG GACCACGCCG GGGCGGCCGA AGGGGGCGGC 
GGGCGGCGGC GAGGCGCGGT GCCTCAGTTC GGGGCGCTGG AACAGGTCAG CTCGTACGCG 
CTGGTGTCGC TGCTCATGCA GCTCTCCGAC CTGTCCCGCT GCGCCGGAGA CATCTTCGGC 
GGGATCCTGA GCGAGGCGGA CACGCTGTGC CACCGCAACG CCCGGCTCCA GCGCCGTCTG 
GGAGCCCTCG AAGCTCTGCT CGCCCGCCTC GACCACCGCA AAGTGAAGAT CCCTGTTTCC 
AACTTAGATG AAGAGAGCAG ATGGACCGTC CATTACACTG CTCCATGGCA CCAGCAGGAA 
AATGTGTTCC TGCCTTCCTC AAGGCCGCCT TGTGTGGAGG ACTTGCACCG GCAAGCAAAG 
CTGAACCTCA AGTCAGTACT GAGAGAGTGT GACAAGCTGC GGAGAGATGG CTATCGGAGC 
TCTCAGTATT ACTCTCAGGG CCCCACCTTC TCCTCATCCT CCAGTGCAAT CTGTGGAAGT 
TACCAGGACG ATTATGAAGA AATTGAACAA AAGTGTTCTG TCTCTTCCCC AGAAGAAGAA 
AAGCTTATCA CCATCAAAAG GCCTAAAACC CCAGTTTCAA ATGACTTATC AGATATCAAC 
ACACAAACCA ACTGGACTAA GTCACTTCCA CTGCCAACAC CAGAAGAGAA GATGCGACAG 
CAAGCACAGG CAGTCCAAAC AGATGTGGTT CCTATCAATG TGACTGTTGG CACAGGCCAA 
AGTGATTTCC GAGGGCACTC GATGTATGTC CCAGATCACA GTTCCACGCT AGGGCGACTA 
GACAGCTATC GCTCTGCTAT GCAGCGGTCT GAAACCAAGG ACACCAGCTG CCAGACTGAA 
GAAGTTAAAG TTGTGCCTCC TTCGATGAGA AGAATACGAG CACAGAAAGG ACAAGGCATT 
GCAGCTCAGA TGTCTCAGTT CTCCAGCTCA TCTGGAAACA TGTCTGTGAT GAGTGATTCT 
GCTGCAGTTA TATTTGCTTC TCGCCAGAAT AGTGACATAG GTTTTCACAG CTTGCCTCGG 
GCTGGTGCAA GAGTGTCTCT GCAGTCCTTA GAACAGACCC AGAGCATCTC CAGACAGACA 
GAAGACATTG CTGGTACTTT GCCTCACCAG ATGAGTAAAC TGCAAGTGGA TGATGATGTT 
GCACATCTGA GAAATAATGC CATAACAGGA ACTGTTTCGA GGCCAAAGTC TCAAGAGGTA 
AGAAGTTGTG ACAGTGAAAA GTCCACTAGC CCAGCATGTG TGGTCTCTCC TCATGCCACC 
TACTCAACAA GCGTCATACC CAATGCGACA CTGTCATCCT CCTCAGAAGT TATTGCTATT 
CACACTGCCC AGAGTACTGG ATCGCTGGAT AACAAAATTA GCAGTTCTGC TGTGTACCCA 
AAACCAAGAG ATAATCCTGT TGCCAACAAT GCAATAAGTG GGAAAGAAGA TCACCATTCT 
TCAAGTGGTA ACTGGAGTGA GAGTAGCTCC ACACGTCACT CACAGACTTC AGATACCATC 
CCATCTCATA CTGTCATGAT GCTTTCTCTT GGTGACTCTG CTGTCTCTCT CAGCACTCCT 
GGAAATGTAG AGGCTGGCTC TCAGAGTGTG AACTACAGCT GCAGAAGCAA TCTTGCTTCA 
CCAAACCAGT CCCAGGACAG CGATGGTAGG AGTGAATCTA GCTACTCAGG GGAGCGAACA 
CAAGGTGTAG TGAGCAGCAC AGAGCACTGG CTATACAAGT CTGCGGAAAA TAGTGAGACT 
GCATCATGCA AGGTAGCTTG TACCACACCA GGCTGTGCCA CTCCTGGTAG CAACCTGAGC 
AGCAGCAGCC TGGAGAGGAC ATCAGCCAAA GAAGATTCCA CTTCACTGTA TTCTGTAGAC 
CACGATGGTT ATTACAGCTC TATGAGCATG GACTCTGGAC TGAAGTCAGA CATGCCATGC 
TATAGTACTA ATGGTTTTGG GAACCCCAGA GACAGCGTGA TTAATGTCTT TGAAGCAAAG 
GAGAAGAAAC ATCAAGATGA CCAGCCAGGC CAAGGTGAAA AATCCCTTGC AAGAAACATC 
TCTCTAAAAA AGGCAAAGAA GCCGCCTTTA CCTCCGTCTA GAACAGACTC ACTCAGAAGG 
GTACCCAAGA AGAAAGCTCA GTCCAATGGA CAGGTACTTG ATGAAACCCT CATTGCTACC 
CTCCAGCATT CTCTGCAGTT GAATCTCAAG TGCAAAAATG GCAGCTCCCC TTCCCAGAGC 
CCCTGCAGTG ACTATGAGGA TCCCTGGGTG TTGCGCTCTC GCAGCCAAAG CTCGGTCAGC 
GCAAGCAGCA GCATGATGTC CACTACTGCC CCAAACCTGT ACTCCATTTG CACAGTCACT 
CCCTCTCAGA GTGAAACAAG CAGCATCAAG TCAGAGTATG CTGACCAGTG GGGTTACTAC 
AGTGACTATG CTGCAGTGGC AGATGACCAG ATGAAGTCTC CAGTGACCCA TTCTGCTAGT 
ACCTCTTCTG CTCTCAGTGA TTACAGCATC AGCCACTTCA GTGATGGCTC AAGGGCTTCT 
GTACCACAAG TGCCCGCTGG ACTGGCCAAA CCAAAGAGCA CTTCTCCAGA GAAGTCCCAC 
CGAGTCACAT CGCCATCCAG TGGGTACTCC AGCCAGTCCA ATACACCCAC TGCACTCACT 
CCAGTGCCTG TGTTTTTAAA ATCTGCGTCA TCAGGAAATG GGAAATCCAA GCTGAAACCT 
AAAGTGCCTG AAAGGAAATC TTCTCTTCTG TCTTCAGTCT CCATTTCTTC ATCCTCTACT 
TCTCTTTCTT CAAATACATC TACTGAAGGG AGTGTGAATG TGAAGAAATT GGACCCCACC 
CCGAGTTCTC CTCCAGATTC TGGTGCACCT CTTCCTCCGC CTCCTCTTCT AGCACCTCCT 
CCACATTGCC TTGAGCTCTC TCCTCCCTCT TCCCCTCCTC CTCCTCCAGC AGAAGCCGTG 
GATATATCAC CATTGCCAGC TTCTCCCACA TTCCCCCCCC CTCCACCAGA AGCTGATGTA 
AATTCTTCCT TTGCTCACAG TGTCCAGTGG TTACCACAAG AAGCTTCCAT CGGTTCGTAC 
TCTTCCCCTG TTCCTCCTCC TGTTTTCCCT TTAGTTGTCC CACCACCAGC CCCACCTCTT 
GATCCCAAAC TGACAAAAGG TGCAATGATA TGCCCACAGT ATTCATTTAA GAAACATGAT 
CAGGAAGATT CTTGCTACAA TGCTGTGAAA CAGCCATTCA GCAAGCAAGA TGCATCAAGA 
CCTGTGATGC CCTTGGTAAC CACCAAAGCA TTGCAAATGG TGCAGCTGAG GTCTATCAGA 
AAAGCAACAG AAGGTGACCC ATCACCTGAA TCTGCTTCTG AAACCATCTC TCAGGAAAAG 
GGTATTGTCA CTTCATCATC ACAGTCTTCC CTAAGGCCAT CTCTCTCACT ACGACTCAGT 
AACAGCTTAG GTGAAGAGGA GATGAAGATT CATGGCACTT CATTTAAAAA CACACCTCAA 
ACACTGGCTC AGAGTTCTCC TCTGGTACTC TCTGACAACG TACCCATGCT TGAGAGCGAT 
CAAAAAGCTG CAGTTACTAT GGGTCTAAGC AAGTCTTTGG AACCAGATTC CCCAGGGTCA 
GCTCCAGAAG CCACACCCAA GTCCCCAGTT CGGAGCGAAG ACTTCCATTC TGGCATGCCA 
CTGCAGGGGT CACCAGCATC TCCAGACAAG ACTCAGGTCG TTTTGCCAAA CAAGAAACCA 
CCTCCCATTT CAAAGAAACC CAAACTGTTC CTTGTTGTAC CACCTCCACA GTTAGATCTC 
ACAGTGGAGA AAATAGCTGA AGTGAGTGAT ACTGTCAGAA GCACATCAAG CCCAACTAAG 
AGAGACGCTG TGCTTACACA CTGCGAGGAG GCGAGAAATT GCCTTACAGA TGGACTCAGC 
TCCAGCGAGA TGGACTCTGG CAGCCTGGTT CCTGAGGGAG GAGCTGCCAG AGTCACCTTC 
TCTGAAGCAG TGGGAGTTGG TGCCTTTGTG GTACAGTCCG CTGCATCACC AGTTCAAGAG 
GAGCCCAGGC AAGAGGAGCA GTCAGTTTTT GATGAAGGAA GCAGCTCAGG CAGCAGCCAA 
GACAGCAGCA GTAACACTGA AGGACACCCG TCTCAGGAGA ACGAAAGTGC CGAGGTGTTT 
GAATCAGAAA CAGCAAATAG TTCATTCCTG CCAAGCAATA ATTATGGGGA AGAGACGGAT 
GGAGTGGCAA CACCAGCGAG ACCGAGGACT ACTGAGGATC TTTTTGCAGC CATTCACAGA 
TCCAAAAGGA AAGTCCTTGG CCGTAAGGAT TCTGAGGACG ATCGTACCCG CAACCATTCT 
CCTTCACCCC CCGTAACACC CACTGGTGCT TCCCCAACCT TATCTACCCT CAAACAGGCA 
GGATCTATTC AGAGAAGCGT TCGCAAGAGC AGCACCAGCA ATGACAACTT CAAAGCCCTG 
CTGCTGAAGA AAGGGAGTCG CTGTGACACC AGTTCCCGCA TGTCAGCAGC AGAGATGTTG 
AAGCACACGG ATCCACGGTT CCACCGAGCC AAGGTGGATG CCTCTCCCGA CCTTTCCGAC 
AGCCCTGCCA GCTGCTCGCC CAGCAAGAGC AAACGGGCCC AAGAGGAGTG GGCCAAGAGT 
GAAGGCCTGA TGCCGAGGAG CATGTCCCTC TCCAGCACTA GGTATGGCCG ATCACGAACA 
CCGCCCTCCG CTGCCAGCAG CAAGTACAAT GTTCGCAACC GCATCCAGAG CAGCCCCATG 
ACAGTGATTA GCGAAGGAGA TGGGGAAGTG GTGGAGCAGA CAGAGAGCAG GGTCCAAAGG 
ACTTTGGATG AGCAGCGAGA GAGGCAGCTC GATGTATTTA ACAGTGATGA AATAGACATG 
AATGACTTTT CGTATTCTGA GGAGCTGGAC TGCAAGGAGA CCTTGGATCC AACCCATCTG 
GACTTAATGA CTCAGCCGGG TACTTCGAGA AAGTACCTAA GTCCTTCAGC TGAAGAAAGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_015284194.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015139680.1 Gallus gallus NHS like 1 (NHSL1), transcript variant X5, mRNA.

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CloneID	OGa33684
Clone ID Related Accession (Same CDS sequence)	XM_015284194.1 , XM_015284194.2
Accession Version	XM_015284194.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	NHS-like protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015284194.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTTCC CCCTGCGGAC CGTGGAGCCT CTGAAGCTGT GCAGGTTGGA GGAAGGCGAC 
GGCGCGGAGC GGGGCGGCCC GGCGCCGGGG GACCACGCCG GGGCGGCCGA AGGGGGCGGC 
GGGCGGCGGC GAGGCGCGGT GCCTCAGTTC GGGGCGCTGG AACAGGTCAG CTCGTACGCG 
CTGGTGTCGC TGCTCATGCA GCTCTCCGAC CTGTCCCGCT GCGCCGGAGA CATCTTCGGC 
GGGATCCTGA GCGAGGCGGA CACGCTGTGC CACCGCAACG CCCGGCTCCA GCGCCGTCTG 
GGAGCCCTCG AAGCTCTGCT CGCCCGCCTC GACCACCGCA AAGTGAAGAT CCCTGTTTCC 
AACTTAGATG AAGAGAGCAG ATGGACCGTC CATTACACTG CTCCATGGCA CCAGCAGGAA 
AATGTGTTCC TGCCTTCCTC AAGGCCGCCT TGTGTGGAGG ACTTGCACCG GCAAGCAAAG 
CTGAACCTCA AGTCAGTACT GAGAGAGTGT GACAAGCTGC GGAGAGATGG CTATCGGAGC 
TCTCAGTATT ACTCTCAGGG CCCCACCTTC TCCTCATCCT CCAGTGCAAT CTGTGGAAGT 
TACCAGGACG ATTATGAAGA AATTGAACAA AAGTGTTCTG TCTCTTCCCC AGAAGAAGAA 
AAGCTTATCA CCATCAAAAG GCCTAAAACC CCAGTTTCAA ATGACTTATC AGATATCAAC 
ACACAAACCA ACTGGACTAA GTCACTTCCA CTGCCAACAC CAGAAGAGAA GATGCGACAG 
CAAGCACAGG CAGTCCAAAC AGATGTGGTT CCTATCAATG TGACTGTTGG CACAGGCCAA 
AGTGATTTCC GAGGGCACTC GATGTATGTC CCAGATCACA GTTCCACGCT AGGGCGACTA 
GACAGCTATC GCTCTGCTAT GCAGCGGTCT GAAACCAAGG ACACCAGCTG CCAGACTGAA 
GAAGTTAAAG TTGTGCCTCC TTCGATGAGA AGAATACGAG CACAGAAAGG ACAAGGCATT 
GCAGCTCAGA TGTCTCAGTT CTCCAGCTCA TCTGGAAACA TGTCTGTGAT GAGTGATTCT 
GCTGCAGTTA TATTTGCTTC TCGCCAGAAT AGTGACATAG GTTTTCACAG CTTGCCTCGG 
GCTGGTGCAA GAGTGTCTCT GCAGTCCTTA GAACAGACCC AGAGCATCTC CAGACAGACA 
GAAGACATTG CTGGTACTTT GCCTCACCAG ATGAGTAAAC TGCAAGTGGA TGATGATGTT 
GCACATCTGA GAAATAATGC CATAACAGGA ACTGTTTCGA GGCCAAAGTC TCAAGAGGTA 
AGAAGTTGTG ACAGTGAAAA GTCCACTAGC CCAGCATGTG TGGTCTCTCC TCATGCCACC 
TACTCAACAA GCGTCATACC CAATGCGACA CTGTCATCCT CCTCAGAAGT TATTGCTATT 
CACACTGCCC AGAGTACTGG ATCGCTGGAT AACAAAATTA GCAGTTCTGC TGTGTACCCA 
AAACCAAGAG ATAATCCTGT TGCCAACAAT GCAATAAGTG GGAAAGAAGA TCACCATTCT 
TCAAGTGGTA ACTGGAGTGA GAGTAGCTCC ACACGTCACT CACAGACTTC AGATACCATC 
CCATCTCATA CTGTCATGAT GCTTTCTCTT GGTGACTCTG CTGTCTCTCT CAGCACTCCT 
GGAAATGTAG AGGCTGGCTC TCAGAGTGTG AACTACAGCT GCAGAAGCAA TCTTGCTTCA 
CCAAACCAGT CCCAGGACAG CGATGGTAGG AGTGAATCTA GCTACTCAGG GGAGCGAACA 
CAAGGTGTAG TGAGCAGCAC AGAGCACTGG CTATACAAGT CTGCGGAAAA TAGTGAGACT 
GCATCATGCA AGGTAGCTTG TACCACACCA GGCTGTGCCA CTCCTGGTAG CAACCTGAGC 
AGCAGCAGCC TGGAGAGGAC ATCAGCCAAA GAAGATTCCA CTTCACTGTA TTCTGTAGAC 
CACGATGGTT ATTACAGCTC TATGAGCATG GACTCTGGAC TGAAGTCAGA CATGCCATGC 
TATAGTACTA ATGGTTTTGG GAACCCCAGA GACAGCGTGA TTAATGTCTT TGAAGCAAAG 
GAGAAGAAAC ATCAAGATGA CCAGCCAGGC CAAGGTGAAA AATCCCTTGC AAGAAACATC 
TCTCTAAAAA AGGCAAAGAA GCCGCCTTTA CCTCCGTCTA GAACAGACTC ACTCAGAAGG 
GTACCCAAGA AGAAAGCTCA GTCCAATGGA CAGGTACTTG ATGAAACCCT CATTGCTACC 
CTCCAGCATT CTCTGCAGTT GAATCTCAAG TGCAAAAATG GCAGCTCCCC TTCCCAGAGC 
CCCTGCAGTG ACTATGAGGA TCCCTGGGTG TTGCGCTCTC GCAGCCAAAG CTCGGTCAGC 
GCAAGCAGCA GCATGATGTC CACTACTGCC CCAAACCTGT ACTCCATTTG CACAGTCACT 
CCCTCTCAGA GTGAAACAAG CAGCATCAAG TCAGAGTATG CTGACCAGTG GGGTTACTAC 
AGTGACTATG CTGCAGTGGC AGATGACCAG ATGAAGTCTC CAGTGACCCA TTCTGCTAGT 
ACCTCTTCTG CTCTCAGTGA TTACAGCATC AGCCACTTCA GTGATGGCTC AAGGGCTTCT 
GTACCACAAG TGCCCGCTGG ACTGGCCAAA CCAAAGAGCA CTTCTCCAGA GAAGTCCCAC 
CGAGTCACAT CGCCATCCAG TGGGTACTCC AGCCAGTCCA ATACACCCAC TGCACTCACT 
CCAGTGCCTG TGTTTTTAAA ATCTGCGTCA TCAGGAAATG GGAAATCCAA GCTGAAACCT 
AAAGTGCCTG AAAGGAAATC TTCTCTTCTG TCTTCAGTCT CCATTTCTTC ATCCTCTACT 
TCTCTTTCTT CAAATACATC TACTGAAGGG AGTGTGAATG TGAAGAAATT GGACCCCACC 
CCGAGTTCTC CTCCAGATTC TGGTGCACCT CTTCCTCCGC CTCCTCTTCT AGCACCTCCT 
CCACATTGCC TTGAGCTCTC TCCTCCCTCT TCCCCTCCTC CTCCTCCAGC AGAAGCCGTG 
GATATATCAC CATTGCCAGC TTCTCCCACA TTCCCCCCCC CTCCACCAGA AGCTGATGTA 
AATTCTTCCT TTGCTCACAG TGTCCAGTGG TTACCACAAG AAGCTTCCAT CGGTTCGTAC 
TCTTCCCCTG TTCCTCCTCC TGTTTTCCCT TTAGTTGTCC CACCACCAGC CCCACCTCTT 
GATCCCAAAC TGACAAAAGG TGCAATGATA TGCCCACAGT ATTCATTTAA GAAACATGAT 
CAGGAAGATT CTTGCTACAA TGCTGTGAAA CAGCCATTCA GCAAGCAAGA TGCATCAAGA 
CCTGTGATGC CCTTGGTAAC CACCAAAGCA TTGCAAATGG TGCAGCTGAG GTCTATCAGA 
AAAGCAACAG AAGGTGACCC ATCACCTGAA TCTGCTTCTG AAACCATCTC TCAGGAAAAG 
GGTATTGTCA CTTCATCATC ACAGTCTTCC CTAAGGCCAT CTCTCTCACT ACGACTCAGT 
AACAGCTTAG GTGAAGAGGA GATGAAGATT CATGGCACTT CATTTAAAAA CACACCTCAA 
ACACTGGCTC AGAGTTCTCC TCTGGTACTC TCTGACAACG TACCCATGCT TGAGAGCGAT 
CAAAAAGCTG CAGTTACTAT GGGTCTAAGC AAGTCTTTGG AACCAGATTC CCCAGGGTCA 
GCTCCAGAAG CCACACCCAA GTCCCCAGTT CGGAGCGAAG ACTTCCATTC TGGCATGCCA 
CTGCAGGGGT CACCAGCATC TCCAGACAAG ACTCAGGTCG TTTTGCCAAA CAAGAAACCA 
CCTCCCATTT CAAAGAAACC CAAACTGTTC CTTGTTGTAC CACCTCCACA GTTAGATCTC 
ACAGTGGAGA AAATAGCTGA AGTGAGTGAT ACTGTCAGAA GCACATCAAG CCCAACTAAG 
AGAGACGCTG TGCTTACACA CTGCGAGGAG GCGAGAAATT GCCTTACAGA TGGACTCAGC 
TCCAGCGAGA TGGACTCTGG CAGCCTGGTT CCTGAGGGAG GAGCTGCCAG AGTCACCTTC 
TCTGAAGCAG TGGGAGTTGG TGCCTTTGTG GTACAGTCCG CTGCATCACC AGTTCAAGAG 
GAGCCCAGGC AAGAGGAGCA GTCAGTTTTT GATGAAGGAA GCAGCTCAGG CAGCAGCCAA 
GACAGCAGCA GTAACACTGA AGGACACCCG TCTCAGGAGA ACGAAAGTGC CGAGGTGTTT 
GAATCAGAAA CAGCAAATAG TTCATTCCTG CCAAGCAATA ATTATGGGGA AGAGACGGAT 
GGAGTGGCAA CACCAGCGAG ACCGAGGACT ACTGAGGATC TTTTTGCAGC CATTCACAGA 
TCCAAAAGGA AAGTCCTTGG CCGTAAGGAT TCTGAGGACG ATCGTACCCG CAACCATTCT 
CCTTCACCCC CCGTAACACC CACTGGTGCT TCCCCAACCT TATCTACCCT CAAACAGGCA 
GGATCTATTC AGAGAAGCGT TCGCAAGAGC AGCACCAGCA ATGACAACTT CAAAGCCCTG 
CTGCTGAAGA AAGGGAGTCG CTGTGACACC AGTTCCCGCA TGTCAGCAGC AGAGATGTTG 
AAGCACACGG ATCCACGGTT CCACCGAGCC AAGGTGGATG CCTCTCCCGA CCTTTCCGAC 
AGCCCTGCCA GCTGCTCGCC CAGCAAGAGC AAACGGGCCC AAGAGGAGTG GGCCAAGAGT 
GAAGGCCTGA TGCCGAGGAG CATGTCCCTC TCCAGCACTA GGTATGGCCG ATCACGAACA 
CCGCCCTCCG CTGCCAGCAG CAAGTACAAT GTTCGCAACC GCATCCAGAG CAGCCCCATG 
ACAGTGATTA GCGAAGGAGA TGGGGAAGTG GTGGAGCAGA CAGAGAGCAG GGTCCAAAGG 
ACTTTGGATG AGCAGCGAGA GAGGCAGCTC GATGTATTTA ACAGTGATGA AATAGACATG 
AATGACTTTT CGTATTCTGA GGAGCTGGAC TGCAAGGAGA CCTTGGATCC AACCCATCTG 
GACTTAATGA CTCAGCCGGG TACTTCGAGA AAGTACCTAA GTCCTTCAGC TGAAGAAAGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015139680.1
CDS	34..5136
Translation	MPFPLRTVEPLKLCRLEEGDGAERGGPAPGDHAGAAEGGGGRRRGAVPQFGALEQVSSYALVSLLMQLSDLSRCAGDIFGGILSEADTLCHRNARLQRRLGALEALLARLDHRKVKIPVSNLDEESRWTVHYTAPWHQQENVFLPSSRPPCVEDLHRQAKLNLKSVLRECDKLRRDGYRSSQYYSQGPTFSSSSSAICGSYQDDYEEIEQKCSVSSPEEEKLITIKRPKTPVSNDLSDINTQTNWTKSLPLPTPEEKMRQQAQAVQTDVVPINVTVGTGQSDFRGHSMYVPDHSSTLGRLDSYRSAMQRSETKDTSCQTEEVKVVPPSMRRIRAQKGQGIAAQMSQFSSSSGNMSVMSDSAAVIFASRQNSDIGFHSLPRAGARVSLQSLEQTQSISRQTEDIAGTLPHQMSKLQVDDDVAHLRNNAITGTVSRPKSQEVRSCDSEKSTSPACVVSPHATYSTSVIPNATLSSSSEVIAIHTAQSTGSLDNKISSSAVYPKPRDNPVANNAISGKEDHHSSSGNWSESSSTRHSQTSDTIPSHTVMMLSLGDSAVSLSTPGNVEAGSQSVNYSCRSNLASPNQSQDSDGRSESSYSGERTQGVVSSTEHWLYKSAENSETASCKVACTTPGCATPGSNLSSSSLERTSAKEDSTSLYSVDHDGYYSSMSMDSGLKSDMPCYSTNGFGNPRDSVINVFEAKEKKHQDDQPGQGEKSLARNISLKKAKKPPLPPSRTDSLRRVPKKKAQSNGQVLDETLIATLQHSLQLNLKCKNGSSPSQSPCSDYEDPWVLRSRSQSSVSASSSMMSTTAPNLYSICTVTPSQSETSSIKSEYADQWGYYSDYAAVADDQMKSPVTHSASTSSALSDYSISHFSDGSRASVPQVPAGLAKPKSTSPEKSHRVTSPSSGYSSQSNTPTALTPVPVFLKSASSGNGKSKLKPKVPERKSSLLSSVSISSSSTSLSSNTSTEGSVNVKKLDPTPSSPPDSGAPLPPPPLLAPPPHCLELSPPSSPPPPPAEAVDISPLPASPTFPPPPPEADVNSSFAHSVQWLPQEASIGSYSSPVPPPVFPLVVPPPAPPLDPKLTKGAMICPQYSFKKHDQEDSCYNAVKQPFSKQDASRPVMPLVTTKALQMVQLRSIRKATEGDPSPESASETISQEKGIVTSSSQSSLRPSLSLRLSNSLGEEEMKIHGTSFKNTPQTLAQSSPLVLSDNVPMLESDQKAAVTMGLSKSLEPDSPGSAPEATPKSPVRSEDFHSGMPLQGSPASPDKTQVVLPNKKPPPISKKPKLFLVVPPPQLDLTVEKIAEVSDTVRSTSSPTKRDAVLTHCEEARNCLTDGLSSSEMDSGSLVPEGGAARVTFSEAVGVGAFVVQSAASPVQEEPRQEEQSVFDEGSSSGSSQDSSSNTEGHPSQENESAEVFESETANSSFLPSNNYGEETDGVATPARPRTTEDLFAAIHRSKRKVLGRKDSEDDRTRNHSPSPPVTPTGASPTLSTLKQAGSIQRSVRKSSTSNDNFKALLLKKGSRCDTSSRMSAAEMLKHTDPRFHRAKVDASPDLSDSPASCSPSKSKRAQEEWAKSEGLMPRSMSLSSTRYGRSRTPPSAASSKYNVRNRIQSSPMTVISEGDGEVVEQTESRVQRTLDEQRERQLDVFNSDEIDMNDFSYSEELDCKETLDPTHLDLMTQPGTSRKYLSPSAEES

Target ORF information:

RefSeq Version	XM_015284194.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NHS like 1 (NHSL1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015284194.2

ORF Insert Sequence:

ATGCCGTTCC CCCTGCGGAC CGTGGAGCCT CTGAAGCTGT GCAGGTTGGA GGAAGGCGAC 
GGCGCGGAGC GGGGCGGCCC GGCGCCGGGG GACCACGCCG GGGCGGCCGA AGGGGGCGGC 
GGGCGGCGGC GAGGCGCGGT GCCTCAGTTC GGGGCGCTGG AACAGGTCAG CTCGTACGCG 
CTGGTGTCGC TGCTCATGCA GCTCTCCGAC CTGTCCCGCT GCGCCGGAGA CATCTTCGGC 
GGGATCCTGA GCGAGGCGGA CACGCTGTGC CACCGCAACG CCCGGCTCCA GCGCCGTCTG 
GGAGCCCTCG AAGCTCTGCT CGCCCGCCTC GACCACCGCA AAGTGAAGAT CCCTGTTTCC 
AACTTAGATG AAGAGAGCAG ATGGACCGTC CATTACACTG CTCCATGGCA CCAGCAGGAA 
AATGTGTTCC TGCCTTCCTC AAGGCCGCCT TGTGTGGAGG ACTTGCACCG GCAAGCAAAG 
CTGAACCTCA AGTCAGTACT GAGAGAGTGT GACAAGCTGC GGAGAGATGG CTATCGGAGC 
TCTCAGTATT ACTCTCAGGG CCCCACCTTC TCCTCATCCT CCAGTGCAAT CTGTGGAAGT 
TACCAGGACG ATTATGAAGA AATTGAACAA AAGTGTTCTG TCTCTTCCCC AGAAGAAGAA 
AAGCTTATCA CCATCAAAAG GCCTAAAACC CCAGTTTCAA ATGACTTATC AGATATCAAC 
ACACAAACCA ACTGGACTAA GTCACTTCCA CTGCCAACAC CAGAAGAGAA GATGCGACAG 
CAAGCACAGG CAGTCCAAAC AGATGTGGTT CCTATCAATG TGACTGTTGG CACAGGCCAA 
AGTGATTTCC GAGGGCACTC GATGTATGTC CCAGATCACA GTTCCACGCT AGGGCGACTA 
GACAGCTATC GCTCTGCTAT GCAGCGGTCT GAAACCAAGG ACACCAGCTG CCAGACTGAA 
GAAGTTAAAG TTGTGCCTCC TTCGATGAGA AGAATACGAG CACAGAAAGG ACAAGGCATT 
GCAGCTCAGA TGTCTCAGTT CTCCAGCTCA TCTGGAAACA TGTCTGTGAT GAGTGATTCT 
GCTGCAGTTA TATTTGCTTC TCGCCAGAAT AGTGACATAG GTTTTCACAG CTTGCCTCGG 
GCTGGTGCAA GAGTGTCTCT GCAGTCCTTA GAACAGACCC AGAGCATCTC CAGACAGACA 
GAAGACATTG CTGGTACTTT GCCTCACCAG ATGAGTAAAC TGCAAGTGGA TGATGATGTT 
GCACATCTGA GAAATAATGC CATAACAGGA ACTGTTTCGA GGCCAAAGTC TCAAGAGGTA 
AGAAGTTGTG ACAGTGAAAA GTCCACTAGC CCAGCATGTG TGGTCTCTCC TCATGCCACC 
TACTCAACAA GCGTCATACC CAATGCGACA CTGTCATCCT CCTCAGAAGT TATTGCTATT 
CACACTGCCC AGAGTACTGG ATCGCTGGAT AACAAAATTA GCAGTTCTGC TGTGTACCCA 
AAACCAAGAG ATAATCCTGT TGCCAACAAT GCAATAAGTG GGAAAGAAGA TCACCATTCT 
TCAAGTGGTA ACTGGAGTGA GAGTAGCTCC ACACGTCACT CACAGACTTC AGATACCATC 
CCATCTCATA CTGTCATGAT GCTTTCTCTT GGTGACTCTG CTGTCTCTCT CAGCACTCCT 
GGAAATGTAG AGGCTGGCTC TCAGAGTGTG AACTACAGCT GCAGAAGCAA TCTTGCTTCA 
CCAAACCAGT CCCAGGACAG CGATGGTAGG AGTGAATCTA GCTACTCAGG GGAGCGAACA 
CAAGGTGTAG TGAGCAGCAC AGAGCACTGG CTATACAAGT CTGCGGAAAA TAGTGAGACT 
GCATCATGCA AGGTAGCTTG TACCACACCA GGCTGTGCCA CTCCTGGTAG CAACCTGAGC 
AGCAGCAGCC TGGAGAGGAC ATCAGCCAAA GAAGATTCCA CTTCACTGTA TTCTGTAGAC 
CACGATGGTT ATTACAGCTC TATGAGCATG GACTCTGGAC TGAAGTCAGA CATGCCATGC 
TATAGTACTA ATGGTTTTGG GAACCCCAGA GACAGCGTGA TTAATGTCTT TGAAGCAAAG 
GAGAAGAAAC ATCAAGATGA CCAGCCAGGC CAAGGTGAAA AATCCCTTGC AAGAAACATC 
TCTCTAAAAA AGGCAAAGAA GCCGCCTTTA CCTCCGTCTA GAACAGACTC ACTCAGAAGG 
GTACCCAAGA AGAAAGCTCA GTCCAATGGA CAGGTACTTG ATGAAACCCT CATTGCTACC 
CTCCAGCATT CTCTGCAGTT GAATCTCAAG TGCAAAAATG GCAGCTCCCC TTCCCAGAGC 
CCCTGCAGTG ACTATGAGGA TCCCTGGGTG TTGCGCTCTC GCAGCCAAAG CTCGGTCAGC 
GCAAGCAGCA GCATGATGTC CACTACTGCC CCAAACCTGT ACTCCATTTG CACAGTCACT 
CCCTCTCAGA GTGAAACAAG CAGCATCAAG TCAGAGTATG CTGACCAGTG GGGTTACTAC 
AGTGACTATG CTGCAGTGGC AGATGACCAG ATGAAGTCTC CAGTGACCCA TTCTGCTAGT 
ACCTCTTCTG CTCTCAGTGA TTACAGCATC AGCCACTTCA GTGATGGCTC AAGGGCTTCT 
GTACCACAAG TGCCCGCTGG ACTGGCCAAA CCAAAGAGCA CTTCTCCAGA GAAGTCCCAC 
CGAGTCACAT CGCCATCCAG TGGGTACTCC AGCCAGTCCA ATACACCCAC TGCACTCACT 
CCAGTGCCTG TGTTTTTAAA ATCTGCGTCA TCAGGAAATG GGAAATCCAA GCTGAAACCT 
AAAGTGCCTG AAAGGAAATC TTCTCTTCTG TCTTCAGTCT CCATTTCTTC ATCCTCTACT 
TCTCTTTCTT CAAATACATC TACTGAAGGG AGTGTGAATG TGAAGAAATT GGACCCCACC 
CCGAGTTCTC CTCCAGATTC TGGTGCACCT CTTCCTCCGC CTCCTCTTCT AGCACCTCCT 
CCACATTGCC TTGAGCTCTC TCCTCCCTCT TCCCCTCCTC CTCCTCCAGC AGAAGCCGTG 
GATATATCAC CATTGCCAGC TTCTCCCACA TTCCCCCCCC CTCCACCAGA AGCTGATGTA 
AATTCTTCCT TTGCTCACAG TGTCCAGTGG TTACCACAAG AAGCTTCCAT CGGTTCGTAC 
TCTTCCCCTG TTCCTCCTCC TGTTTTCCCT TTAGTTGTCC CACCACCAGC CCCACCTCTT 
GATCCCAAAC TGACAAAAGG TGCAATGATA TGCCCACAGT ATTCATTTAA GAAACATGAT 
CAGGAAGATT CTTGCTACAA TGCTGTGAAA CAGCCATTCA GCAAGCAAGA TGCATCAAGA 
CCTGTGATGC CCTTGGTAAC CACCAAAGCA TTGCAAATGG TGCAGCTGAG GTCTATCAGA 
AAAGCAACAG AAGGTGACCC ATCACCTGAA TCTGCTTCTG AAACCATCTC TCAGGAAAAG 
GGTATTGTCA CTTCATCATC ACAGTCTTCC CTAAGGCCAT CTCTCTCACT ACGACTCAGT 
AACAGCTTAG GTGAAGAGGA GATGAAGATT CATGGCACTT CATTTAAAAA CACACCTCAA 
ACACTGGCTC AGAGTTCTCC TCTGGTACTC TCTGACAACG TACCCATGCT TGAGAGCGAT 
CAAAAAGCTG CAGTTACTAT GGGTCTAAGC AAGTCTTTGG AACCAGATTC CCCAGGGTCA 
GCTCCAGAAG CCACACCCAA GTCCCCAGTT CGGAGCGAAG ACTTCCATTC TGGCATGCCA 
CTGCAGGGGT CACCAGCATC TCCAGACAAG ACTCAGGTCG TTTTGCCAAA CAAGAAACCA 
CCTCCCATTT CAAAGAAACC CAAACTGTTC CTTGTTGTAC CACCTCCACA GTTAGATCTC 
ACAGTGGAGA AAATAGCTGA AGTGAGTGAT ACTGTCAGAA GCACATCAAG CCCAACTAAG 
AGAGACGCTG TGCTTACACA CTGCGAGGAG GCGAGAAATT GCCTTACAGA TGGACTCAGC 
TCCAGCGAGA TGGACTCTGG CAGCCTGGTT CCTGAGGGAG GAGCTGCCAG AGTCACCTTC 
TCTGAAGCAG TGGGAGTTGG TGCCTTTGTG GTACAGTCCG CTGCATCACC AGTTCAAGAG 
GAGCCCAGGC AAGAGGAGCA GTCAGTTTTT GATGAAGGAA GCAGCTCAGG CAGCAGCCAA 
GACAGCAGCA GTAACACTGA AGGACACCCG TCTCAGGAGA ACGAAAGTGC CGAGGTGTTT 
GAATCAGAAA CAGCAAATAG TTCATTCCTG CCAAGCAATA ATTATGGGGA AGAGACGGAT 
GGAGTGGCAA CACCAGCGAG ACCGAGGACT ACTGAGGATC TTTTTGCAGC CATTCACAGA 
TCCAAAAGGA AAGTCCTTGG CCGTAAGGAT TCTGAGGACG ATCGTACCCG CAACCATTCT 
CCTTCACCCC CCGTAACACC CACTGGTGCT TCCCCAACCT TATCTACCCT CAAACAGGCA 
GGATCTATTC AGAGAAGCGT TCGCAAGAGC AGCACCAGCA ATGACAACTT CAAAGCCCTG 
CTGCTGAAGA AAGGGAGTCG CTGTGACACC AGTTCCCGCA TGTCAGCAGC AGAGATGTTG 
AAGCACACGG ATCCACGGTT CCACCGAGCC AAGGTGGATG CCTCTCCCGA CCTTTCCGAC 
AGCCCTGCCA GCTGCTCGCC CAGCAAGAGC AAACGGGCCC AAGAGGAGTG GGCCAAGAGT 
GAAGGCCTGA TGCCGAGGAG CATGTCCCTC TCCAGCACTA GGTATGGCCG ATCACGAACA 
CCGCCCTCCG CTGCCAGCAG CAAGTACAAT GTTCGCAACC GCATCCAGAG CAGCCCCATG 
ACAGTGATTA GCGAAGGAGA TGGGGAAGTG GTGGAGCAGA CAGAGAGCAG GGTCCAAAGG 
ACTTTGGATG AGCAGCGAGA GAGGCAGCTC GATGTATTTA ACAGTGATGA AATAGACATG 
AATGACTTTT CGTATTCTGA GGAGCTGGAC TGCAAGGAGA CCTTGGATCC AACCCATCTG 
GACTTAATGA CTCAGCCGGG TACTTCGAGA AAGTACCTAA GTCCTTCAGC TGAAGAAAGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_015284195.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015139681.1 Gallus gallus NHS-like 1 (NHSL1), transcript variant X10, mRNA.

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CloneID	OGa33685
Clone ID Related Accession (Same CDS sequence)	XM_015284195.1 , XM_015284195.2
Accession Version	XM_015284195.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	NHS-like protein 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455837.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTTCC CCCTGCGGAC CGTGGAGCCT CTGAAGCTGT GCAGGTTGGA GGAAGGCGAC 
GGCGCGGAGC GGGGCGGCCC GGCGCCGGGG GACCACGCCG GGGCGGCCGA AGGGGGCGGC 
GGGCGGCGGC GAGGCGCGGT GCCTCAGTTC GGGGCGCTGG AACAGGTCAG CTCGTACGCG 
CTGGTGTCGC TGCTCATGCA GCTCTCCGAC CTGTCCCGCT GCGCCGGAGA CATCTTCGGC 
GGGATCCTGA GCGAGGCGGA CACGCTGTGC CACCGCAACG CCCGGCTCCA GCGCCGTCTG 
GGAGCCCTCG AAGCTCTGCT CGCCCGCCTC GACCACCGCA AAGTGAAGAT CCCTGTTTCC 
AACTTAGATG AAGAGAGCAG ATGGACCGTC CATTACACTG CTCCATGGCA CCAGCAGGAA 
AATGTGTTCC TGCCTTCCTC AAGGCCGCCT TGTGTGGAGG ACTTGCACCG GCAAGCAAAG 
CTGAACCTCA AGTCAGTACT GAGAGAGTGT GACAAGCTGC GGAGAGATGG CTATCGGAGC 
TCTCAGTATT ACTCTCAGGG CCCCACCTTC TCCTCATCCT CCAGTGCAAT CTGTGGAAGT 
TACCAGGACG ATTATGAAGA AATTGAACAA AAGTGTTCTG TCTCTTCCCC AGAAGAAGAA 
AAGCTTATCA CCATCAAAAG GCCTAAAACC CCAGTTTCAA ATGACTTATC AGATATCAAC 
ACACAAACCA ACTGGACTAA GTCACTTCCA CTGCCAACAC CAGAAGAGAA GATGCGACAG 
CAAGCACAGG CAGTCCAAAC AGATGTGGTT CCTATCAATG TGACTGGGGA GAATTTCGAC 
CGCCAAGCCA GCATTCGGCG GTCTCTAATT TACACAGACA CGGTGGTAAG ACGGCCGAAG 
AAAGTCAAAA GGAGAAAGAC TATAACAGGA ATCCCTGACA ACATACAAAA GGAGCTAGCA 
GTTGGCACAG GCCAAAGTGA TTTCCGAGGG CACTCGATGT ATGTCCCAGA TCACAGTTCC 
ACGCTAGGGC GACTAGACAG CTATCGCTCT GCTATGCAGC GGTCTGAAAC CAAGGACACC 
AGCTGCCAGA CTGAAGAAGT TAAAGTTGTG CCTCCTTCGA TGAGAAGAAT ACGAGCACAG 
AAAGGACAAG GCATTGCAGC TCAGATGTCT CAGTTCTCCA GCTCATCTGG AAACATGTCT 
GTGATGAGTG ATTCTGCTGC AGTTATATTT GCTTCTCGCC AGAATAGTGA CATAGGTTTT 
CACAGCTTGC CTCGGGCTGG TGCAAGAGTG TCTCTGCAGT CCTTAGAACA GACCCAGAGC 
ATCTCCAGAC AGACAGAAGA CATTGCTGGT ACTTTGCCTC ACCAGATGAG TAAACTGCAA 
GTGGATGATG ATGTTGCACA TCTGAGAAAT AATGCCATAA CAGGAACTGT TTCGAGGCCA 
AAGTCTCAAG AGGTAAGAAG TTGTGACAGT GAAAAGTCCA CTAGCCCAGC ATGTGTGGTC 
TCTCCTCATG CCACCTACTC AACAAGCGTC ATACCCAATG CGACACTGTC ATCCTCCTCA 
GAAGTTATTG CTATTCACAC TGCCCAGAGT ACTGGATCGC TGGATAACAA AATTAGCAGT 
TCTGCTGTGT ACCCAAAACC AAGAGATAAT CCTGTTGCCA ACAATGCAAT AAGTGGGAAA 
GAAGATCACC ATTCTTCAAG TGGTAACTGG AGTGAGAGTA GCTCCACACG TCACTCACAG 
ACTTCAGATA CCATCCCATC TCATACTGTC ATGATGCTTT CTCTTGGTGA CTCTGCTGTC 
TCTCTCAGCA CTCCTGGAAA TGTAGAGGCT GGCTCTCAGA GTGTGAACTA CAGCTGCAGA 
AGCAATCTTG CTTCACCAAA CCAGTCCCAG GACAGCGATG GTAGGAGTGA ATCTAGCTAC 
TCAGGGGAGC GAACACAAGG TGTAGTGAGC AGCACAGAGC ACTGGCTATA CAAGTCTGCG 
GAAAATAGTG AGACTGCATC ATGCAAGGTA GCTTGTACCA CACCAGGCTG TGCCACTCCT 
GGTAGCAACC TGAGCAGCAG CAGCCTGGAG AGGACATCAG CCAAAGAAGA TTCCACTTCA 
CTGTATTCTG TAGACCACGA TGGTTATTAC AGCTCTATGA GCATGGACTC TGGACTGAAG 
TCAGACATGC CATGCTATAG TACTAATGGT TTTGGGAACC CCAGAGACAG CGTGATTAAT 
GTCTTTGAAG CAAAGGAGAA GAAACATCAA GATGACCAGC CAGGCCAAGG TGAAAAATCC 
CTTGCAAGAA ACATCTCTCT AAAAAAGGCA AAGAAGCCGC CTTTACCTCC GTCTAGAACA 
GACTCACTCA GAAGGGTACC CAAGAAGAAA GCTCAGTCCA ATGGACAGGT ACTTGATGAA 
ACCCTCATTG CTACCCTCCA GCATTCTCTG CAGTTGAATC TCAAGTGCAA AAATGGCAGC 
TCCCCTTCCC AGAGCCCCTG CAGTGACTAT GAGGATCCCT GGGTGTTGCG CTCTCGCAGC 
CAAAGCTCGG TCAGCGCAAG CAGCAGCATG ATGTCCACTA CTGCCCCAAA CCTGTACTCC 
ATTTGCACAG TCACTCCCTC TCAGAGTGAA ACAAGCAGCA TCAAGTCAGA GTATGCTGAC 
CAGTGGGGTT ACTACAGTGA CTATGCTGCA GTGGCAGATG ACCAGATGAA GTCTCCAGTG 
ACCCATTCTG CTAGTACCTC TTCTGCTCTC AGTGATTACA GCATCAGCCA CTTCAGTGAT 
GGCTCAAGGG CTTCTGTACC ACAAGTGCCC GCTGGACTGG CCAAACCAAA GAGCACTTCT 
CCAGAGAAGT CCCACCGAGT CACATCGCCA TCCAGTGGGT ACTCCAGCCA GTCCAATACA 
CCCACTGCAC TCACTCCAGT GCCTGTGTTT TTAAAATCTG CGTCATCAGG AAATGGGAAA 
TCCAAGCTGA AACCTAAAGT GCCTGAAAGG AAATCTTCTC TTCTGTCTTC AGTCTCCATT 
TCTTCATCCT CTACTTCTCT TTCTTCAAAT ACATCTACTG AAGGGAGTGT GAATGTGAAG 
AAATTGGACC CCACCCCGAG TTCTCCTCCA GATTCTGGTG CACCTCTTCC TCCGCCTCCT 
CTTCTAGCAC CTCCTCCACA TTGCCTTGAG CTCTCTCCTC CCTCTTCCCC TCCTCCTCCT 
CCAGCAGAAG CCGTGGATAT ATCACCATTG CCAGCTTCTC CCACATTCCC CCCCCCTCCA 
CCAGAAGCTG ATGTAAATTC TTCCTTTGCT CACAGTGTCC AGTGGTTACC ACAAGAAGCT 
TCCATCGGTT CGTACTCTTC CCCTGTTCCT CCTCCTGTTT TCCCTTTAGT TGTCCCACCA 
CCAGCCCCAC CTCTTGATCC CAAACTGACA AAAGGTGCAA TGATATGCCC ACAGTATTCA 
TTTAAGAAAC ATGATCAGGA AGATTCTTGC TACAATGCTG TGAAACAGCC ATTCAGCAAG 
CAAGATGCAT CAAGACCTGT GATGCCCTTG GTAACCACCA AAGCATTGCA AATGGTGCAG 
CTGAGGTCTA TCAGAAAAGC AACAGAAGGT GACCCATCAC CTGAATCTGC TTCTGAAACC 
ATCTCTCAGG AAAAGGGTAT TGTCACTTCA TCATCACAGT CTTCCCTAAG GCCATCTCTC 
TCACTACGAC TCAGTAACAG CTTAGGTGAA GAGGAGATGA AGATTCATGG CACTTCATTT 
AAAAACACAC CTCAAACACT GGCTCAGAGT TCTCCTCTGG TACTCTCTGA CAACGTACCC 
ATGCTTGAGA GCGATCAAAA AGCTGCAGTT ACTATGGGTC TAAGCAAGTC TTTGGAACCA 
GATTCCCCAG GGTCAGCTCC AGAAGCCACA CCCAAGTCCC CAGTTCGGAG CGAAGACTTC 
CATTCTGGCA TGCCACTGCA GGGGTCACCA GCATCTCCAG ACAAGACTCA GGTCGTTTTG 
CCAAACAAGA AACCACCTCC CATTTCAAAG AAACCCAAAC TGTTCCTTGT TGTACCACCT 
CCACAGTTAG ATCTCACAGT GGAGAAAATA GCTGAAGTGA GTGATACTGT CAGAAGCACA 
TCAAGCCCAA CTAAGAGAGA CGCTGTGCTT ACACACTGCG AGGAGGCGAG AAATTGCCTT 
ACAGATGGAC TCAGCTCCAG CGAGATGGAC TCTGGCAGCC TGGTTCCTGA GGGAGGAGCT 
GCCAGAGTCA CCTTCTCTGA AGCAGTGGGA GTTGGTGCCT TTGTGGTACA GTCCGCTGCA 
TCACCAGTTC AAGAGGAGCC CAGGCAAGAG GAGCAGTCAG TTTTTGATGA AGGAAGCAGC 
TCAGGCAGCA GCCAAGACAG CAGCAGTAAC ACTGAAGGAC ACCCGTCTCA GGAGAACGAA 
AGTGCCGAGG TGTTTGAATC AGAAACAGCA AATAGTTCAT TCCTGCCAAG CAATAATTAT 
GGGGAAGAGA CGGATGGAGT GGCAACACCA GCGAGACCGA GGACTACTGA GGATCTTTTT 
GCAGCCATTC ACAGTTTGGG ACAGAGGCTC AACGCTAATG CTTCATGGCA AGCGTGGCTG 
AAACTGATCC AAAAGGAAAG TCCTTGGCCG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015139681.1
CDS	111..4703
Translation	MPFPLRTVEPLKLCRLEEGDGAERGGPAPGDHAGAAEGGGGRRRGAVPQFGALEQVSSYALVSLLMQLSDLSRCAGDIFGGILSEADTLCHRNARLQRRLGALEALLARLDHRKVKIPVSNLDEESRWTVHYTAPWHQQENVFLPSSRPPCVEDLHRQAKLNLKSVLRECDKLRRDGYRSSQYYSQGPTFSSSSSAICGSYQDDYEEIEQKCSVSSPEEEKLITIKRPKTPVSNDLSDINTQTNWTKSLPLPTPEEKMRQQAQAVQTDVVPINVTGENFDRQASIRRSLIYTDTVVRRPKKVKRRKTITGIPDNIQKELAVGTGQSDFRGHSMYVPDHSSTLGRLDSYRSAMQRSETKDTSCQTEEVKVVPPSMRRIRAQKGQGIAAQMSQFSSSSGNMSVMSDSAAVIFASRQNSDIGFHSLPRAGARVSLQSLEQTQSISRQTEDIAGTLPHQMSKLQVDDDVAHLRNNAITGTVSRPKSQEVRSCDSEKSTSPACVVSPHATYSTSVIPNATLSSSSEVIAIHTAQSTGSLDNKISSSAVYPKPRDNPVANNAISGKEDHHSSSGNWSESSSTRHSQTSDTIPSHTVMMLSLGDSAVSLSTPGNVEAGSQSVNYSCRSNLASPNQSQDSDGRSESSYSGERTQGVVSSTEHWLYKSAENSETASCKVACTTPGCATPGSNLSSSSLERTSAKEDSTSLYSVDHDGYYSSMSMDSGLKSDMPCYSTNGFGNPRDSVINVFEAKEKKHQDDQPGQGEKSLARNISLKKAKKPPLPPSRTDSLRRVPKKKAQSNGQVLDETLIATLQHSLQLNLKCKNGSSPSQSPCSDYEDPWVLRSRSQSSVSASSSMMSTTAPNLYSICTVTPSQSETSSIKSEYADQWGYYSDYAAVADDQMKSPVTHSASTSSALSDYSISHFSDGSRASVPQVPAGLAKPKSTSPEKSHRVTSPSSGYSSQSNTPTALTPVPVFLKSASSGNGKSKLKPKVPERKSSLLSSVSISSSSTSLSSNTSTEGSVNVKKLDPTPSSPPDSGAPLPPPPLLAPPPHCLELSPPSSPPPPPAEAVDISPLPASPTFPPPPPEADVNSSFAHSVQWLPQEASIGSYSSPVPPPVFPLVVPPPAPPLDPKLTKGAMICPQYSFKKHDQEDSCYNAVKQPFSKQDASRPVMPLVTTKALQMVQLRSIRKATEGDPSPESASETISQEKGIVTSSSQSSLRPSLSLRLSNSLGEEEMKIHGTSFKNTPQTLAQSSPLVLSDNVPMLESDQKAAVTMGLSKSLEPDSPGSAPEATPKSPVRSEDFHSGMPLQGSPASPDKTQVVLPNKKPPPISKKPKLFLVVPPPQLDLTVEKIAEVSDTVRSTSSPTKRDAVLTHCEEARNCLTDGLSSSEMDSGSLVPEGGAARVTFSEAVGVGAFVVQSAASPVQEEPRQEEQSVFDEGSSSGSSQDSSSNTEGHPSQENESAEVFESETANSSFLPSNNYGEETDGVATPARPRTTEDLFAAIHSLGQRLNANASWQAWLKLIQKESPWP

Target ORF information:

RefSeq Version	XM_015284195.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NHS-like 1 (NHSL1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015284195.1

ORF Insert Sequence:

ATGCCGTTCC CCCTGCGGAC CGTGGAGCCT CTGAAGCTGT GCAGGTTGGA GGAAGGCGAC 
GGCGCGGAGC GGGGCGGCCC GGCGCCGGGG GACCACGCCG GGGCGGCCGA AGGGGGCGGC 
GGGCGGCGGC GAGGCGCGGT GCCTCAGTTC GGGGCGCTGG AACAGGTCAG CTCGTACGCG 
CTGGTGTCGC TGCTCATGCA GCTCTCCGAC CTGTCCCGCT GCGCCGGAGA CATCTTCGGC 
GGGATCCTGA GCGAGGCGGA CACGCTGTGC CACCGCAACG CCCGGCTCCA GCGCCGTCTG 
GGAGCCCTCG AAGCTCTGCT CGCCCGCCTC GACCACCGCA AAGTGAAGAT CCCTGTTTCC 
AACTTAGATG AAGAGAGCAG ATGGACCGTC CATTACACTG CTCCATGGCA CCAGCAGGAA 
AATGTGTTCC TGCCTTCCTC AAGGCCGCCT TGTGTGGAGG ACTTGCACCG GCAAGCAAAG 
CTGAACCTCA AGTCAGTACT GAGAGAGTGT GACAAGCTGC GGAGAGATGG CTATCGGAGC 
TCTCAGTATT ACTCTCAGGG CCCCACCTTC TCCTCATCCT CCAGTGCAAT CTGTGGAAGT 
TACCAGGACG ATTATGAAGA AATTGAACAA AAGTGTTCTG TCTCTTCCCC AGAAGAAGAA 
AAGCTTATCA CCATCAAAAG GCCTAAAACC CCAGTTTCAA ATGACTTATC AGATATCAAC 
ACACAAACCA ACTGGACTAA GTCACTTCCA CTGCCAACAC CAGAAGAGAA GATGCGACAG 
CAAGCACAGG CAGTCCAAAC AGATGTGGTT CCTATCAATG TGACTGGGGA GAATTTCGAC 
CGCCAAGCCA GCATTCGGCG GTCTCTAATT TACACAGACA CGGTGGTAAG ACGGCCGAAG 
AAAGTCAAAA GGAGAAAGAC TATAACAGGA ATCCCTGACA ACATACAAAA GGAGCTAGCA 
GTTGGCACAG GCCAAAGTGA TTTCCGAGGG CACTCGATGT ATGTCCCAGA TCACAGTTCC 
ACGCTAGGGC GACTAGACAG CTATCGCTCT GCTATGCAGC GGTCTGAAAC CAAGGACACC 
AGCTGCCAGA CTGAAGAAGT TAAAGTTGTG CCTCCTTCGA TGAGAAGAAT ACGAGCACAG 
AAAGGACAAG GCATTGCAGC TCAGATGTCT CAGTTCTCCA GCTCATCTGG AAACATGTCT 
GTGATGAGTG ATTCTGCTGC AGTTATATTT GCTTCTCGCC AGAATAGTGA CATAGGTTTT 
CACAGCTTGC CTCGGGCTGG TGCAAGAGTG TCTCTGCAGT CCTTAGAACA GACCCAGAGC 
ATCTCCAGAC AGACAGAAGA CATTGCTGGT ACTTTGCCTC ACCAGATGAG TAAACTGCAA 
GTGGATGATG ATGTTGCACA TCTGAGAAAT AATGCCATAA CAGGAACTGT TTCGAGGCCA 
AAGTCTCAAG AGGTAAGAAG TTGTGACAGT GAAAAGTCCA CTAGCCCAGC ATGTGTGGTC 
TCTCCTCATG CCACCTACTC AACAAGCGTC ATACCCAATG CGACACTGTC ATCCTCCTCA 
GAAGTTATTG CTATTCACAC TGCCCAGAGT ACTGGATCGC TGGATAACAA AATTAGCAGT 
TCTGCTGTGT ACCCAAAACC AAGAGATAAT CCTGTTGCCA ACAATGCAAT AAGTGGGAAA 
GAAGATCACC ATTCTTCAAG TGGTAACTGG AGTGAGAGTA GCTCCACACG TCACTCACAG 
ACTTCAGATA CCATCCCATC TCATACTGTC ATGATGCTTT CTCTTGGTGA CTCTGCTGTC 
TCTCTCAGCA CTCCTGGAAA TGTAGAGGCT GGCTCTCAGA GTGTGAACTA CAGCTGCAGA 
AGCAATCTTG CTTCACCAAA CCAGTCCCAG GACAGCGATG GTAGGAGTGA ATCTAGCTAC 
TCAGGGGAGC GAACACAAGG TGTAGTGAGC AGCACAGAGC ACTGGCTATA CAAGTCTGCG 
GAAAATAGTG AGACTGCATC ATGCAAGGTA GCTTGTACCA CACCAGGCTG TGCCACTCCT 
GGTAGCAACC TGAGCAGCAG CAGCCTGGAG AGGACATCAG CCAAAGAAGA TTCCACTTCA 
CTGTATTCTG TAGACCACGA TGGTTATTAC AGCTCTATGA GCATGGACTC TGGACTGAAG 
TCAGACATGC CATGCTATAG TACTAATGGT TTTGGGAACC CCAGAGACAG CGTGATTAAT 
GTCTTTGAAG CAAAGGAGAA GAAACATCAA GATGACCAGC CAGGCCAAGG TGAAAAATCC 
CTTGCAAGAA ACATCTCTCT AAAAAAGGCA AAGAAGCCGC CTTTACCTCC GTCTAGAACA 
GACTCACTCA GAAGGGTACC CAAGAAGAAA GCTCAGTCCA ATGGACAGGT ACTTGATGAA 
ACCCTCATTG CTACCCTCCA GCATTCTCTG CAGTTGAATC TCAAGTGCAA AAATGGCAGC 
TCCCCTTCCC AGAGCCCCTG CAGTGACTAT GAGGATCCCT GGGTGTTGCG CTCTCGCAGC 
CAAAGCTCGG TCAGCGCAAG CAGCAGCATG ATGTCCACTA CTGCCCCAAA CCTGTACTCC 
ATTTGCACAG TCACTCCCTC TCAGAGTGAA ACAAGCAGCA TCAAGTCAGA GTATGCTGAC 
CAGTGGGGTT ACTACAGTGA CTATGCTGCA GTGGCAGATG ACCAGATGAA GTCTCCAGTG 
ACCCATTCTG CTAGTACCTC TTCTGCTCTC AGTGATTACA GCATCAGCCA CTTCAGTGAT 
GGCTCAAGGG CTTCTGTACC ACAAGTGCCC GCTGGACTGG CCAAACCAAA GAGCACTTCT 
CCAGAGAAGT CCCACCGAGT CACATCGCCA TCCAGTGGGT ACTCCAGCCA GTCCAATACA 
CCCACTGCAC TCACTCCAGT GCCTGTGTTT TTAAAATCTG CGTCATCAGG AAATGGGAAA 
TCCAAGCTGA AACCTAAAGT GCCTGAAAGG AAATCTTCTC TTCTGTCTTC AGTCTCCATT 
TCTTCATCCT CTACTTCTCT TTCTTCAAAT ACATCTACTG AAGGGAGTGT GAATGTGAAG 
AAATTGGACC CCACCCCGAG TTCTCCTCCA GATTCTGGTG CACCTCTTCC TCCGCCTCCT 
CTTCTAGCAC CTCCTCCACA TTGCCTTGAG CTCTCTCCTC CCTCTTCCCC TCCTCCTCCT 
CCAGCAGAAG CCGTGGATAT ATCACCATTG CCAGCTTCTC CCACATTCCC CCCCCCTCCA 
CCAGAAGCTG ATGTAAATTC TTCCTTTGCT CACAGTGTCC AGTGGTTACC ACAAGAAGCT 
TCCATCGGTT CGTACTCTTC CCCTGTTCCT CCTCCTGTTT TCCCTTTAGT TGTCCCACCA 
CCAGCCCCAC CTCTTGATCC CAAACTGACA AAAGGTGCAA TGATATGCCC ACAGTATTCA 
TTTAAGAAAC ATGATCAGGA AGATTCTTGC TACAATGCTG TGAAACAGCC ATTCAGCAAG 
CAAGATGCAT CAAGACCTGT GATGCCCTTG GTAACCACCA AAGCATTGCA AATGGTGCAG 
CTGAGGTCTA TCAGAAAAGC AACAGAAGGT GACCCATCAC CTGAATCTGC TTCTGAAACC 
ATCTCTCAGG AAAAGGGTAT TGTCACTTCA TCATCACAGT CTTCCCTAAG GCCATCTCTC 
TCACTACGAC TCAGTAACAG CTTAGGTGAA GAGGAGATGA AGATTCATGG CACTTCATTT 
AAAAACACAC CTCAAACACT GGCTCAGAGT TCTCCTCTGG TACTCTCTGA CAACGTACCC 
ATGCTTGAGA GCGATCAAAA AGCTGCAGTT ACTATGGGTC TAAGCAAGTC TTTGGAACCA 
GATTCCCCAG GGTCAGCTCC AGAAGCCACA CCCAAGTCCC CAGTTCGGAG CGAAGACTTC 
CATTCTGGCA TGCCACTGCA GGGGTCACCA GCATCTCCAG ACAAGACTCA GGTCGTTTTG 
CCAAACAAGA AACCACCTCC CATTTCAAAG AAACCCAAAC TGTTCCTTGT TGTACCACCT 
CCACAGTTAG ATCTCACAGT GGAGAAAATA GCTGAAGTGA GTGATACTGT CAGAAGCACA 
TCAAGCCCAA CTAAGAGAGA CGCTGTGCTT ACACACTGCG AGGAGGCGAG AAATTGCCTT 
ACAGATGGAC TCAGCTCCAG CGAGATGGAC TCTGGCAGCC TGGTTCCTGA GGGAGGAGCT 
GCCAGAGTCA CCTTCTCTGA AGCAGTGGGA GTTGGTGCCT TTGTGGTACA GTCCGCTGCA 
TCACCAGTTC AAGAGGAGCC CAGGCAAGAG GAGCAGTCAG TTTTTGATGA AGGAAGCAGC 
TCAGGCAGCA GCCAAGACAG CAGCAGTAAC ACTGAAGGAC ACCCGTCTCA GGAGAACGAA 
AGTGCCGAGG TGTTTGAATC AGAAACAGCA AATAGTTCAT TCCTGCCAAG CAATAATTAT 
GGGGAAGAGA CGGATGGAGT GGCAACACCA GCGAGACCGA GGACTACTGA GGATCTTTTT 
GCAGCCATTC ACAGTTTGGG ACAGAGGCTC AACGCTAATG CTTCATGGCA AGCGTGGCTG 
AAACTGATCC AAAAGGAAAG TCCTTGGCCG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_015284195.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015139681.1 Gallus gallus NHS like 1 (NHSL1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OGa33685
Clone ID Related Accession (Same CDS sequence)	XM_015284195.1 , XM_015284195.2
Accession Version	XM_015284195.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	NHS-like protein 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015284195.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTTCC CCCTGCGGAC CGTGGAGCCT CTGAAGCTGT GCAGGTTGGA GGAAGGCGAC 
GGCGCGGAGC GGGGCGGCCC GGCGCCGGGG GACCACGCCG GGGCGGCCGA AGGGGGCGGC 
GGGCGGCGGC GAGGCGCGGT GCCTCAGTTC GGGGCGCTGG AACAGGTCAG CTCGTACGCG 
CTGGTGTCGC TGCTCATGCA GCTCTCCGAC CTGTCCCGCT GCGCCGGAGA CATCTTCGGC 
GGGATCCTGA GCGAGGCGGA CACGCTGTGC CACCGCAACG CCCGGCTCCA GCGCCGTCTG 
GGAGCCCTCG AAGCTCTGCT CGCCCGCCTC GACCACCGCA AAGTGAAGAT CCCTGTTTCC 
AACTTAGATG AAGAGAGCAG ATGGACCGTC CATTACACTG CTCCATGGCA CCAGCAGGAA 
AATGTGTTCC TGCCTTCCTC AAGGCCGCCT TGTGTGGAGG ACTTGCACCG GCAAGCAAAG 
CTGAACCTCA AGTCAGTACT GAGAGAGTGT GACAAGCTGC GGAGAGATGG CTATCGGAGC 
TCTCAGTATT ACTCTCAGGG CCCCACCTTC TCCTCATCCT CCAGTGCAAT CTGTGGAAGT 
TACCAGGACG ATTATGAAGA AATTGAACAA AAGTGTTCTG TCTCTTCCCC AGAAGAAGAA 
AAGCTTATCA CCATCAAAAG GCCTAAAACC CCAGTTTCAA ATGACTTATC AGATATCAAC 
ACACAAACCA ACTGGACTAA GTCACTTCCA CTGCCAACAC CAGAAGAGAA GATGCGACAG 
CAAGCACAGG CAGTCCAAAC AGATGTGGTT CCTATCAATG TGACTGGGGA GAATTTCGAC 
CGCCAAGCCA GCATTCGGCG GTCTCTAATT TACACAGACA CGGTGGTAAG ACGGCCGAAG 
AAAGTCAAAA GGAGAAAGAC TATAACAGGA ATCCCTGACA ACATACAAAA GGAGCTAGCA 
GTTGGCACAG GCCAAAGTGA TTTCCGAGGG CACTCGATGT ATGTCCCAGA TCACAGTTCC 
ACGCTAGGGC GACTAGACAG CTATCGCTCT GCTATGCAGC GGTCTGAAAC CAAGGACACC 
AGCTGCCAGA CTGAAGAAGT TAAAGTTGTG CCTCCTTCGA TGAGAAGAAT ACGAGCACAG 
AAAGGACAAG GCATTGCAGC TCAGATGTCT CAGTTCTCCA GCTCATCTGG AAACATGTCT 
GTGATGAGTG ATTCTGCTGC AGTTATATTT GCTTCTCGCC AGAATAGTGA CATAGGTTTT 
CACAGCTTGC CTCGGGCTGG TGCAAGAGTG TCTCTGCAGT CCTTAGAACA GACCCAGAGC 
ATCTCCAGAC AGACAGAAGA CATTGCTGGT ACTTTGCCTC ACCAGATGAG TAAACTGCAA 
GTGGATGATG ATGTTGCACA TCTGAGAAAT AATGCCATAA CAGGAACTGT TTCGAGGCCA 
AAGTCTCAAG AGGTAAGAAG TTGTGACAGT GAAAAGTCCA CTAGCCCAGC ATGTGTGGTC 
TCTCCTCATG CCACCTACTC AACAAGCGTC ATACCCAATG CGACACTGTC ATCCTCCTCA 
GAAGTTATTG CTATTCACAC TGCCCAGAGT ACTGGATCGC TGGATAACAA AATTAGCAGT 
TCTGCTGTGT ACCCAAAACC AAGAGATAAT CCTGTTGCCA ACAATGCAAT AAGTGGGAAA 
GAAGATCACC ATTCTTCAAG TGGTAACTGG AGTGAGAGTA GCTCCACACG TCACTCACAG 
ACTTCAGATA CCATCCCATC TCATACTGTC ATGATGCTTT CTCTTGGTGA CTCTGCTGTC 
TCTCTCAGCA CTCCTGGAAA TGTAGAGGCT GGCTCTCAGA GTGTGAACTA CAGCTGCAGA 
AGCAATCTTG CTTCACCAAA CCAGTCCCAG GACAGCGATG GTAGGAGTGA ATCTAGCTAC 
TCAGGGGAGC GAACACAAGG TGTAGTGAGC AGCACAGAGC ACTGGCTATA CAAGTCTGCG 
GAAAATAGTG AGACTGCATC ATGCAAGGTA GCTTGTACCA CACCAGGCTG TGCCACTCCT 
GGTAGCAACC TGAGCAGCAG CAGCCTGGAG AGGACATCAG CCAAAGAAGA TTCCACTTCA 
CTGTATTCTG TAGACCACGA TGGTTATTAC AGCTCTATGA GCATGGACTC TGGACTGAAG 
TCAGACATGC CATGCTATAG TACTAATGGT TTTGGGAACC CCAGAGACAG CGTGATTAAT 
GTCTTTGAAG CAAAGGAGAA GAAACATCAA GATGACCAGC CAGGCCAAGG TGAAAAATCC 
CTTGCAAGAA ACATCTCTCT AAAAAAGGCA AAGAAGCCGC CTTTACCTCC GTCTAGAACA 
GACTCACTCA GAAGGGTACC CAAGAAGAAA GCTCAGTCCA ATGGACAGGT ACTTGATGAA 
ACCCTCATTG CTACCCTCCA GCATTCTCTG CAGTTGAATC TCAAGTGCAA AAATGGCAGC 
TCCCCTTCCC AGAGCCCCTG CAGTGACTAT GAGGATCCCT GGGTGTTGCG CTCTCGCAGC 
CAAAGCTCGG TCAGCGCAAG CAGCAGCATG ATGTCCACTA CTGCCCCAAA CCTGTACTCC 
ATTTGCACAG TCACTCCCTC TCAGAGTGAA ACAAGCAGCA TCAAGTCAGA GTATGCTGAC 
CAGTGGGGTT ACTACAGTGA CTATGCTGCA GTGGCAGATG ACCAGATGAA GTCTCCAGTG 
ACCCATTCTG CTAGTACCTC TTCTGCTCTC AGTGATTACA GCATCAGCCA CTTCAGTGAT 
GGCTCAAGGG CTTCTGTACC ACAAGTGCCC GCTGGACTGG CCAAACCAAA GAGCACTTCT 
CCAGAGAAGT CCCACCGAGT CACATCGCCA TCCAGTGGGT ACTCCAGCCA GTCCAATACA 
CCCACTGCAC TCACTCCAGT GCCTGTGTTT TTAAAATCTG CGTCATCAGG AAATGGGAAA 
TCCAAGCTGA AACCTAAAGT GCCTGAAAGG AAATCTTCTC TTCTGTCTTC AGTCTCCATT 
TCTTCATCCT CTACTTCTCT TTCTTCAAAT ACATCTACTG AAGGGAGTGT GAATGTGAAG 
AAATTGGACC CCACCCCGAG TTCTCCTCCA GATTCTGGTG CACCTCTTCC TCCGCCTCCT 
CTTCTAGCAC CTCCTCCACA TTGCCTTGAG CTCTCTCCTC CCTCTTCCCC TCCTCCTCCT 
CCAGCAGAAG CCGTGGATAT ATCACCATTG CCAGCTTCTC CCACATTCCC CCCCCCTCCA 
CCAGAAGCTG ATGTAAATTC TTCCTTTGCT CACAGTGTCC AGTGGTTACC ACAAGAAGCT 
TCCATCGGTT CGTACTCTTC CCCTGTTCCT CCTCCTGTTT TCCCTTTAGT TGTCCCACCA 
CCAGCCCCAC CTCTTGATCC CAAACTGACA AAAGGTGCAA TGATATGCCC ACAGTATTCA 
TTTAAGAAAC ATGATCAGGA AGATTCTTGC TACAATGCTG TGAAACAGCC ATTCAGCAAG 
CAAGATGCAT CAAGACCTGT GATGCCCTTG GTAACCACCA AAGCATTGCA AATGGTGCAG 
CTGAGGTCTA TCAGAAAAGC AACAGAAGGT GACCCATCAC CTGAATCTGC TTCTGAAACC 
ATCTCTCAGG AAAAGGGTAT TGTCACTTCA TCATCACAGT CTTCCCTAAG GCCATCTCTC 
TCACTACGAC TCAGTAACAG CTTAGGTGAA GAGGAGATGA AGATTCATGG CACTTCATTT 
AAAAACACAC CTCAAACACT GGCTCAGAGT TCTCCTCTGG TACTCTCTGA CAACGTACCC 
ATGCTTGAGA GCGATCAAAA AGCTGCAGTT ACTATGGGTC TAAGCAAGTC TTTGGAACCA 
GATTCCCCAG GGTCAGCTCC AGAAGCCACA CCCAAGTCCC CAGTTCGGAG CGAAGACTTC 
CATTCTGGCA TGCCACTGCA GGGGTCACCA GCATCTCCAG ACAAGACTCA GGTCGTTTTG 
CCAAACAAGA AACCACCTCC CATTTCAAAG AAACCCAAAC TGTTCCTTGT TGTACCACCT 
CCACAGTTAG ATCTCACAGT GGAGAAAATA GCTGAAGTGA GTGATACTGT CAGAAGCACA 
TCAAGCCCAA CTAAGAGAGA CGCTGTGCTT ACACACTGCG AGGAGGCGAG AAATTGCCTT 
ACAGATGGAC TCAGCTCCAG CGAGATGGAC TCTGGCAGCC TGGTTCCTGA GGGAGGAGCT 
GCCAGAGTCA CCTTCTCTGA AGCAGTGGGA GTTGGTGCCT TTGTGGTACA GTCCGCTGCA 
TCACCAGTTC AAGAGGAGCC CAGGCAAGAG GAGCAGTCAG TTTTTGATGA AGGAAGCAGC 
TCAGGCAGCA GCCAAGACAG CAGCAGTAAC ACTGAAGGAC ACCCGTCTCA GGAGAACGAA 
AGTGCCGAGG TGTTTGAATC AGAAACAGCA AATAGTTCAT TCCTGCCAAG CAATAATTAT 
GGGGAAGAGA CGGATGGAGT GGCAACACCA GCGAGACCGA GGACTACTGA GGATCTTTTT 
GCAGCCATTC ACAGTTTGGG ACAGAGGCTC AACGCTAATG CTTCATGGCA AGCGTGGCTG 
AAACTGATCC AAAAGGAAAG TCCTTGGCCG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015139681.1
CDS	34..4626
Translation	MPFPLRTVEPLKLCRLEEGDGAERGGPAPGDHAGAAEGGGGRRRGAVPQFGALEQVSSYALVSLLMQLSDLSRCAGDIFGGILSEADTLCHRNARLQRRLGALEALLARLDHRKVKIPVSNLDEESRWTVHYTAPWHQQENVFLPSSRPPCVEDLHRQAKLNLKSVLRECDKLRRDGYRSSQYYSQGPTFSSSSSAICGSYQDDYEEIEQKCSVSSPEEEKLITIKRPKTPVSNDLSDINTQTNWTKSLPLPTPEEKMRQQAQAVQTDVVPINVTGENFDRQASIRRSLIYTDTVVRRPKKVKRRKTITGIPDNIQKELAVGTGQSDFRGHSMYVPDHSSTLGRLDSYRSAMQRSETKDTSCQTEEVKVVPPSMRRIRAQKGQGIAAQMSQFSSSSGNMSVMSDSAAVIFASRQNSDIGFHSLPRAGARVSLQSLEQTQSISRQTEDIAGTLPHQMSKLQVDDDVAHLRNNAITGTVSRPKSQEVRSCDSEKSTSPACVVSPHATYSTSVIPNATLSSSSEVIAIHTAQSTGSLDNKISSSAVYPKPRDNPVANNAISGKEDHHSSSGNWSESSSTRHSQTSDTIPSHTVMMLSLGDSAVSLSTPGNVEAGSQSVNYSCRSNLASPNQSQDSDGRSESSYSGERTQGVVSSTEHWLYKSAENSETASCKVACTTPGCATPGSNLSSSSLERTSAKEDSTSLYSVDHDGYYSSMSMDSGLKSDMPCYSTNGFGNPRDSVINVFEAKEKKHQDDQPGQGEKSLARNISLKKAKKPPLPPSRTDSLRRVPKKKAQSNGQVLDETLIATLQHSLQLNLKCKNGSSPSQSPCSDYEDPWVLRSRSQSSVSASSSMMSTTAPNLYSICTVTPSQSETSSIKSEYADQWGYYSDYAAVADDQMKSPVTHSASTSSALSDYSISHFSDGSRASVPQVPAGLAKPKSTSPEKSHRVTSPSSGYSSQSNTPTALTPVPVFLKSASSGNGKSKLKPKVPERKSSLLSSVSISSSSTSLSSNTSTEGSVNVKKLDPTPSSPPDSGAPLPPPPLLAPPPHCLELSPPSSPPPPPAEAVDISPLPASPTFPPPPPEADVNSSFAHSVQWLPQEASIGSYSSPVPPPVFPLVVPPPAPPLDPKLTKGAMICPQYSFKKHDQEDSCYNAVKQPFSKQDASRPVMPLVTTKALQMVQLRSIRKATEGDPSPESASETISQEKGIVTSSSQSSLRPSLSLRLSNSLGEEEMKIHGTSFKNTPQTLAQSSPLVLSDNVPMLESDQKAAVTMGLSKSLEPDSPGSAPEATPKSPVRSEDFHSGMPLQGSPASPDKTQVVLPNKKPPPISKKPKLFLVVPPPQLDLTVEKIAEVSDTVRSTSSPTKRDAVLTHCEEARNCLTDGLSSSEMDSGSLVPEGGAARVTFSEAVGVGAFVVQSAASPVQEEPRQEEQSVFDEGSSSGSSQDSSSNTEGHPSQENESAEVFESETANSSFLPSNNYGEETDGVATPARPRTTEDLFAAIHSLGQRLNANASWQAWLKLIQKESPWP

Target ORF information:

RefSeq Version	XM_015284195.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NHS like 1 (NHSL1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015284195.2

ORF Insert Sequence:

ATGCCGTTCC CCCTGCGGAC CGTGGAGCCT CTGAAGCTGT GCAGGTTGGA GGAAGGCGAC 
GGCGCGGAGC GGGGCGGCCC GGCGCCGGGG GACCACGCCG GGGCGGCCGA AGGGGGCGGC 
GGGCGGCGGC GAGGCGCGGT GCCTCAGTTC GGGGCGCTGG AACAGGTCAG CTCGTACGCG 
CTGGTGTCGC TGCTCATGCA GCTCTCCGAC CTGTCCCGCT GCGCCGGAGA CATCTTCGGC 
GGGATCCTGA GCGAGGCGGA CACGCTGTGC CACCGCAACG CCCGGCTCCA GCGCCGTCTG 
GGAGCCCTCG AAGCTCTGCT CGCCCGCCTC GACCACCGCA AAGTGAAGAT CCCTGTTTCC 
AACTTAGATG AAGAGAGCAG ATGGACCGTC CATTACACTG CTCCATGGCA CCAGCAGGAA 
AATGTGTTCC TGCCTTCCTC AAGGCCGCCT TGTGTGGAGG ACTTGCACCG GCAAGCAAAG 
CTGAACCTCA AGTCAGTACT GAGAGAGTGT GACAAGCTGC GGAGAGATGG CTATCGGAGC 
TCTCAGTATT ACTCTCAGGG CCCCACCTTC TCCTCATCCT CCAGTGCAAT CTGTGGAAGT 
TACCAGGACG ATTATGAAGA AATTGAACAA AAGTGTTCTG TCTCTTCCCC AGAAGAAGAA 
AAGCTTATCA CCATCAAAAG GCCTAAAACC CCAGTTTCAA ATGACTTATC AGATATCAAC 
ACACAAACCA ACTGGACTAA GTCACTTCCA CTGCCAACAC CAGAAGAGAA GATGCGACAG 
CAAGCACAGG CAGTCCAAAC AGATGTGGTT CCTATCAATG TGACTGGGGA GAATTTCGAC 
CGCCAAGCCA GCATTCGGCG GTCTCTAATT TACACAGACA CGGTGGTAAG ACGGCCGAAG 
AAAGTCAAAA GGAGAAAGAC TATAACAGGA ATCCCTGACA ACATACAAAA GGAGCTAGCA 
GTTGGCACAG GCCAAAGTGA TTTCCGAGGG CACTCGATGT ATGTCCCAGA TCACAGTTCC 
ACGCTAGGGC GACTAGACAG CTATCGCTCT GCTATGCAGC GGTCTGAAAC CAAGGACACC 
AGCTGCCAGA CTGAAGAAGT TAAAGTTGTG CCTCCTTCGA TGAGAAGAAT ACGAGCACAG 
AAAGGACAAG GCATTGCAGC TCAGATGTCT CAGTTCTCCA GCTCATCTGG AAACATGTCT 
GTGATGAGTG ATTCTGCTGC AGTTATATTT GCTTCTCGCC AGAATAGTGA CATAGGTTTT 
CACAGCTTGC CTCGGGCTGG TGCAAGAGTG TCTCTGCAGT CCTTAGAACA GACCCAGAGC 
ATCTCCAGAC AGACAGAAGA CATTGCTGGT ACTTTGCCTC ACCAGATGAG TAAACTGCAA 
GTGGATGATG ATGTTGCACA TCTGAGAAAT AATGCCATAA CAGGAACTGT TTCGAGGCCA 
AAGTCTCAAG AGGTAAGAAG TTGTGACAGT GAAAAGTCCA CTAGCCCAGC ATGTGTGGTC 
TCTCCTCATG CCACCTACTC AACAAGCGTC ATACCCAATG CGACACTGTC ATCCTCCTCA 
GAAGTTATTG CTATTCACAC TGCCCAGAGT ACTGGATCGC TGGATAACAA AATTAGCAGT 
TCTGCTGTGT ACCCAAAACC AAGAGATAAT CCTGTTGCCA ACAATGCAAT AAGTGGGAAA 
GAAGATCACC ATTCTTCAAG TGGTAACTGG AGTGAGAGTA GCTCCACACG TCACTCACAG 
ACTTCAGATA CCATCCCATC TCATACTGTC ATGATGCTTT CTCTTGGTGA CTCTGCTGTC 
TCTCTCAGCA CTCCTGGAAA TGTAGAGGCT GGCTCTCAGA GTGTGAACTA CAGCTGCAGA 
AGCAATCTTG CTTCACCAAA CCAGTCCCAG GACAGCGATG GTAGGAGTGA ATCTAGCTAC 
TCAGGGGAGC GAACACAAGG TGTAGTGAGC AGCACAGAGC ACTGGCTATA CAAGTCTGCG 
GAAAATAGTG AGACTGCATC ATGCAAGGTA GCTTGTACCA CACCAGGCTG TGCCACTCCT 
GGTAGCAACC TGAGCAGCAG CAGCCTGGAG AGGACATCAG CCAAAGAAGA TTCCACTTCA 
CTGTATTCTG TAGACCACGA TGGTTATTAC AGCTCTATGA GCATGGACTC TGGACTGAAG 
TCAGACATGC CATGCTATAG TACTAATGGT TTTGGGAACC CCAGAGACAG CGTGATTAAT 
GTCTTTGAAG CAAAGGAGAA GAAACATCAA GATGACCAGC CAGGCCAAGG TGAAAAATCC 
CTTGCAAGAA ACATCTCTCT AAAAAAGGCA AAGAAGCCGC CTTTACCTCC GTCTAGAACA 
GACTCACTCA GAAGGGTACC CAAGAAGAAA GCTCAGTCCA ATGGACAGGT ACTTGATGAA 
ACCCTCATTG CTACCCTCCA GCATTCTCTG CAGTTGAATC TCAAGTGCAA AAATGGCAGC 
TCCCCTTCCC AGAGCCCCTG CAGTGACTAT GAGGATCCCT GGGTGTTGCG CTCTCGCAGC 
CAAAGCTCGG TCAGCGCAAG CAGCAGCATG ATGTCCACTA CTGCCCCAAA CCTGTACTCC 
ATTTGCACAG TCACTCCCTC TCAGAGTGAA ACAAGCAGCA TCAAGTCAGA GTATGCTGAC 
CAGTGGGGTT ACTACAGTGA CTATGCTGCA GTGGCAGATG ACCAGATGAA GTCTCCAGTG 
ACCCATTCTG CTAGTACCTC TTCTGCTCTC AGTGATTACA GCATCAGCCA CTTCAGTGAT 
GGCTCAAGGG CTTCTGTACC ACAAGTGCCC GCTGGACTGG CCAAACCAAA GAGCACTTCT 
CCAGAGAAGT CCCACCGAGT CACATCGCCA TCCAGTGGGT ACTCCAGCCA GTCCAATACA 
CCCACTGCAC TCACTCCAGT GCCTGTGTTT TTAAAATCTG CGTCATCAGG AAATGGGAAA 
TCCAAGCTGA AACCTAAAGT GCCTGAAAGG AAATCTTCTC TTCTGTCTTC AGTCTCCATT 
TCTTCATCCT CTACTTCTCT TTCTTCAAAT ACATCTACTG AAGGGAGTGT GAATGTGAAG 
AAATTGGACC CCACCCCGAG TTCTCCTCCA GATTCTGGTG CACCTCTTCC TCCGCCTCCT 
CTTCTAGCAC CTCCTCCACA TTGCCTTGAG CTCTCTCCTC CCTCTTCCCC TCCTCCTCCT 
CCAGCAGAAG CCGTGGATAT ATCACCATTG CCAGCTTCTC CCACATTCCC CCCCCCTCCA 
CCAGAAGCTG ATGTAAATTC TTCCTTTGCT CACAGTGTCC AGTGGTTACC ACAAGAAGCT 
TCCATCGGTT CGTACTCTTC CCCTGTTCCT CCTCCTGTTT TCCCTTTAGT TGTCCCACCA 
CCAGCCCCAC CTCTTGATCC CAAACTGACA AAAGGTGCAA TGATATGCCC ACAGTATTCA 
TTTAAGAAAC ATGATCAGGA AGATTCTTGC TACAATGCTG TGAAACAGCC ATTCAGCAAG 
CAAGATGCAT CAAGACCTGT GATGCCCTTG GTAACCACCA AAGCATTGCA AATGGTGCAG 
CTGAGGTCTA TCAGAAAAGC AACAGAAGGT GACCCATCAC CTGAATCTGC TTCTGAAACC 
ATCTCTCAGG AAAAGGGTAT TGTCACTTCA TCATCACAGT CTTCCCTAAG GCCATCTCTC 
TCACTACGAC TCAGTAACAG CTTAGGTGAA GAGGAGATGA AGATTCATGG CACTTCATTT 
AAAAACACAC CTCAAACACT GGCTCAGAGT TCTCCTCTGG TACTCTCTGA CAACGTACCC 
ATGCTTGAGA GCGATCAAAA AGCTGCAGTT ACTATGGGTC TAAGCAAGTC TTTGGAACCA 
GATTCCCCAG GGTCAGCTCC AGAAGCCACA CCCAAGTCCC CAGTTCGGAG CGAAGACTTC 
CATTCTGGCA TGCCACTGCA GGGGTCACCA GCATCTCCAG ACAAGACTCA GGTCGTTTTG 
CCAAACAAGA AACCACCTCC CATTTCAAAG AAACCCAAAC TGTTCCTTGT TGTACCACCT 
CCACAGTTAG ATCTCACAGT GGAGAAAATA GCTGAAGTGA GTGATACTGT CAGAAGCACA 
TCAAGCCCAA CTAAGAGAGA CGCTGTGCTT ACACACTGCG AGGAGGCGAG AAATTGCCTT 
ACAGATGGAC TCAGCTCCAG CGAGATGGAC TCTGGCAGCC TGGTTCCTGA GGGAGGAGCT 
GCCAGAGTCA CCTTCTCTGA AGCAGTGGGA GTTGGTGCCT TTGTGGTACA GTCCGCTGCA 
TCACCAGTTC AAGAGGAGCC CAGGCAAGAG GAGCAGTCAG TTTTTGATGA AGGAAGCAGC 
TCAGGCAGCA GCCAAGACAG CAGCAGTAAC ACTGAAGGAC ACCCGTCTCA GGAGAACGAA 
AGTGCCGAGG TGTTTGAATC AGAAACAGCA AATAGTTCAT TCCTGCCAAG CAATAATTAT 
GGGGAAGAGA CGGATGGAGT GGCAACACCA GCGAGACCGA GGACTACTGA GGATCTTTTT 
GCAGCCATTC ACAGTTTGGG ACAGAGGCTC AACGCTAATG CTTCATGGCA AGCGTGGCTG 
AAACTGATCC AAAAGGAAAG TCCTTGGCCG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	NHSL1
Entrez Gene ID	421680
Full Name	NHS like 1
General protein information	Preferred Names NHS like 1 Names NHS-like protein 1
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

NHSL1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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