MAP3K4 cDNA ORF clone, Gallus gallus(chicken)

The following MAP3K4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAP3K4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa33777 XM_015284321.2
Latest version!
Gallus gallus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa33777 XM_025148975.1
Latest version!
Gallus gallus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa33780 XM_015284325.2
Latest version!
Gallus gallus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa33780 XM_015284324.2
Latest version!
Gallus gallus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa33775 XM_015284319.1
Latest version!
Gallus gallus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OGa33776 XM_015284320.1
Latest version!
Gallus gallus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OGa33778 XM_015284322.1
Latest version!
Gallus gallus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OGa33779 XM_015284323.1
Latest version!
Gallus gallus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OGa33781 XM_015284326.1
Latest version!
Gallus gallus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
View Old Accession Versions >>
OGa33780 XM_015284325.1 Gallus gallus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa33777 XM_015284321.1 Gallus gallus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa33780 XM_015284324.1 Gallus gallus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa33777
    Clone ID Related Accession (Same CDS sequence) XM_015284321.2 , XM_015284321.1 , XM_025148975.1
    Accession Version XM_015284321.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4680bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product mitogen-activated protein kinase kinase kinase 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015284321.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAATCTGC ATATTTCCAG GAAAGAGTGC AAATCAGAAG ATGCTACTTG CAAGATCTCT 
    GAATCTGAAC CTGAGGAGTT ATCAGATGAA GCAAGTGCTG ACACCTTCTA TGGAACTTCT
    CCTCCTAGTA CACCCCGCCA GATGAAGCGT ATGTCAACAA AGCATCAGAA AAACAACGTC
    GGGAGACCTG CTAGCCGCTC TACTTTGAAA GAAAAGATGA GTATACCACC TCAATCCGTG
    CATAAAGACA ATGGGAAAAC CATTGAGAAC GTGGAGGAGC ACAGCTATAA GCAAGGGAAA
    AAAATGCGTG ACACCCTAAG GACGACAGAA CGAGATCACA AGAAAAATGT GCAGTGCTCG
    TTTATGTTAG ACTCAGTTGG CGGGTCTCTG CCAAAAAAAA CAATTCCAGA TGTTGATCTC
    AATAAGCCTT ACCTCAGCCT TGGCTGTAGC AATGCTAAGC TTCCAGTCTC TGTGCCCATG
    CCAATCTCCA GAACTGCACG CCAGACTTCT AGGACTGATT GCCCAGCGGA CCGCTTAAAA
    TTCTTTGAAA CCCTGCGGCT TTTGTTAAAA CTTACCTCAG TCTCGAAAAA AAAGGACAGG
    GAGCAAAGAG GACAGGAAAG CACCTCTTCT GTCTGGCTGA ACCGATCCAA TGAACTGATC
    TGGCTAGAGC TGCAAGCTTG GCATGCTGGC CGGAGTATTA ATGACCAAGA CCTTTATCTC
    TATGCAGCCC GTCAAGCTAT CCCTGATATT ATCAATGAAA TCCTTACATT CAAAGTGAAC
    TATGGAAGCT TCTCCTATGT AAAAAATGGA GCCAGTCTCA ATGGTACGTC AGGAGAAGGA
    GTTTGCAAAG CAACACATGG AACCAGTGCT TTGGGTTACT CAAGTTACCA CGAACACCTC
    CATCGCCAGC GAATCTCATT TGAGCAAGTA AAGCGAATAA TGGAACTGCT AGAGTATATA
    GAAGCACTTT ATCCATCTCT GCAGGCTCTT CAAAAGGACT ATGAAAAGTA TGCTGCAAAA
    GACTTCCAGG ACAGAGTGCA GGCACTCTGT CTGTGGTTGA ACATTACAAA AGACTTAAAT
    CAAAAGCTAA GGATTATGGG GACTGTTTTG GGCATCAAAA ATCTCTCTGA CATTGGCTGG
    CCAGTGTTTG AAATTCCTTC TCCTCGACCG TCTAAGGACG ATGATCCAGA GGATGAAGGA
    GTTGATAGGG AAACTGAAGT AAAGGAATCC TCAGGAACAT GCACAGAGGA GAGCGATGGC
    GAAGAACAAA TCTCTGAGGA GAATGTTGAG GAACTTAGAC AAGCCATCAA GAACAATTTT
    ACTAATCAGC CAAATTTCGA CTTTCAGGAC CATTTTTCAA GGAAGCTTGA TAGGCTTGAA
    TCTGGGGATG ACTCTGCCTC TTGGATTATT CCAGAATGCA GTGCTGAGGC AGGCTGCAGC
    AAACACTGTT TGACTTCTAT TTACAGGCCG TTTGTAGACA AAGCACTGAA GCAAATGGGG
    TTGAGGAAGC TAATTTTAAG ACTACACAAG CTAATGCATG GTTCATTGCA AAGGGCTCGT
    ATGGCGTTGG TAAGGAGTGA TTGCCCGCTG GAGTTTTCAG AATGTCCAGA TCCCATGTTG
    TGTTCAGATT ACGTGCAGTT ATTAAATTCC CCACCTTGCT GTGAGCAAAA ATGCAGCACT
    GTATCATGGG AAGAGCTCAA ATCCATGGAT TTGCCATCTT TTGAACCTGC ATTCTTGGTC
    CTTTGCCGAG TCCTGCTCAA TGTTATCCAT GAATGTCTCA AGCTAAGGTT GGAACAAAGG
    CCTGCTGGGG AGCCATCTCT TTTGAGTATT AAACAGCTAG TGAGGGAGTG TAAGGAGGTT
    CTGAAGGGTG GCCTGTTAAT GAAGCAATAT TACCAATTTA TGTTGCGTGA GGTGTTAGAT
    GACTTGCAAA AGATTGATTG CAACATTGAT GCTTTTGAAG AGGACCTGCA TAAAATGTTG
    ATGGTTTACT TTGATTACAT GCGAAGCTGG ATCCAAATGC TACAGCAGTT ACCTCAAGCA
    TCTCATAGTT TAAAAAATCT CCTAGAGGAA GAATGGAATT TCACCAAAGA AATAACTCCA
    TATATAAGAG GAGGAGAAGC TCAAGCTGGA AAACTATTTT GTGATATTGC AGGAATGCTG
    CTGAAATCTA CAGGAATATT TTTAGATTCT GGACTACAAG ACAGTTGTGA TGAATTTTGG
    GCCAGTGCCG ATGACAGTAC TGCATCAGAT GAAATAAGGA GGTCTGTCAT AGAGACCAGC
    CGAGCACTCA AGGAACTGTT TCATGAAGCT AGAGAAAGGG CCTCCAAGGC TCTTGGATTT
    GCTAAAATGC TGAGGAAGGA CCTTGAAATA GCAGCAGAGT TTGCATTGTC AGCCCCTGTC
    CGAGATCTTC TGAATGCTCT GAAAACGAAA GAGTATGTGA AGGTACAAAT CCCTGGACTC
    GAGAATTTAC AAGTATTTGT CCCAAATAGT ATTGCAGGTG AAAAGTCTGT GATTTTGCAG
    CTCCTAAATG CGGCTGCTGG GAAAGATTGC TCAAAAGATT CAGATGAGGT TGCATATGAG
    GCCTATTTAA TAATGACCAA ACACAGTGAC AAAGACCATG AATTAGGTGA CATGTGGAGT
    GCATGGGAGG GCCAACCAGT CAAAGTGGTG CCACAAGTGG AAACTGTTGA TACGCTACGA
    AGCATGCAGG TTGACAACCT CCTTCTCGTA GTCATTCAGT CTGCCCACCT TGTGAGTCAG
    CGAAAAGCTT TCCAACAGTC TATAGAAGGG CTCATATCAC TCCACCAGGA ACAGACATCC
    AGCCAACCAG TCATTGCCAA AGCTCTGCAG CAACTAAAGA ATGATGCATT GCAGCTATGC
    AATAAGATAA GCAGTGCCAT TGACAGAGTT GATCATATGT TCACATCTGA GTTTGATGCT
    GAAGTTGATG AATCTGAATC TGCGACTTTG CAGCAATACT ACCGGGAAGC CATGATTCAG
    GCTTACAATT TTGGGTTTGA GTACCACAAA GAAGTTGTTC GTCTGATGTC TGGAGAATTC
    AGGCAGAAAA TTGGAGACAA ATATATCAGC TTTGCCAGAA AGTGGATGAA CTACGTGCTA
    ACAAAATGTG AGAGTGGCCG AGGCACTCGA CCCAGGTGGG CAACTCAAGG TTTTGATTTT
    CTTCAAGCCA TTGAACCAGC ATTTATTTCG GCTCTCCCAG AAGATGATTT CTTGAGTTTG
    CAAGCTCTGA TGAATGAGTG CATTGGCCAC GTTATTGGAA AACCTCACAG TCCTGTTACA
    GGTCTGTACC TTGCTATTCA TCGGAACAGT CCCCGTCCCG TGAAAGTACC TCGATGCCAT
    AGTGATCCAC CGAATCCTCA TCTTATCATC CCCACTCCTG AAGGCTTCAG CACACGGAGC
    GTCCCTTGCG ATGCACGGAA CCCTTGCAAC GTTACTGTCA GTCGCCCCGC TCCCATTGGT
    AGTGACTCAA CTCAACCTAA ATCTGTCAGC AGTGCAAATG ATACCAGGGG TTCCAGTGTC
    CCCGAAAATG ATCGACTGGC TTCAATAGCA GCCGAGTTGC AGTTCAGATC CCTTAGTCGT
    CACTCCAGTC CCACTGAGGA GCGAGAAGAA CCATCATATC CAAAAGGAGA TTCAAGTGGC
    TCATCCCGGC GGAGTTGGGA GCTCAGAACC TTAATCAACC AGACCAAAGA CACTGTTTCC
    AAACAAGCAC CAATAGAAGC TGTTCAGAAG TCTGTTCAGT TGTTTGAAGA AAGGAGATAC
    AGAGAGATGA GGAGGAAGAA TATCATTGGC CAAGTTTGCG ACACACCAAA ATCCTACGAT
    AATGTCATGC ATGTAGGGTT GAGAAAAGTG ACTTTCAAGT GGCAGAGAGG AAACAAAATT
    GGTGAAGGCC AATATGGGAA AGTATATACC TGCATTAGTG TTGACACTGG AGAGCTGATG
    GCTATGAAAG AAATAAGATT TCAACCTAAT GATCACAAAA CCATCAAAGA AACAGCTGAT
    GAACTGAAGA TTTTTGAAGG CATCAAACAC CCTAATCTTG TTCGGTATTT TGGTGTGGAG
    CTCCATAGGG AAGAGATGTA CATCTTCATG GAGTACTGTG ATGAGGGCAC GCTGGAGGAG
    GTGTCAAAAC TGGGCCTTCA GGAGCATGTC ATTAGGCTCT ACACAAAGCA GATCACCATT
    GCTATCAATG TACTACATGA ACACGGTATT GTTCATCGAG ACATTAAAGG AGCCAACATC
    TTTCTTACCT CATCCGGACT GATTAAACTG GGTGACTTTG GCTGCTCAGT AAAACTAAAG
    AACAACACTC AAACAATGCC TGGAGAAGTG AACAGTACAT TGGGTACTGC AGCGTACATG
    GCTCCAGAAG TAATTACTAG AGCAAAAGGA GAAGGGCATG GACGTGCAGC AGACATCTGG
    AGCCTGGGCT GTGTTGTTAT AGAGATGGTC ACTGGCAAGA GGCCTTGGCA CGAATATGAG
    CACAACTTCC AGATCATGTA TAGAGTGGGG ATGGGTCATA AACCTCCTAT TCCTGACAAA
    GTGAGTCCGG AGGGGAAAGA CTTCCTTTGC CACTGCCTGG AAAGTGACCC AAAGATGAGG
    TGGACAGCCA GCCAGCTCCT CGACCATCCT TTTGTCAAGG TTTGCACAGA TGAAGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139807.1
    CDS378..5057
    Translation

    Target ORF information:

    RefSeq Version XM_015284321.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284321.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAATCTGC ATATTTCCAG GAAAGAGTGC AAATCAGAAG ATGCTACTTG CAAGATCTCT 
    GAATCTGAAC CTGAGGAGTT ATCAGATGAA GCAAGTGCTG ACACCTTCTA TGGAACTTCT
    CCTCCTAGTA CACCCCGCCA GATGAAGCGT ATGTCAACAA AGCATCAGAA AAACAACGTC
    GGGAGACCTG CTAGCCGCTC TACTTTGAAA GAAAAGATGA GTATACCACC TCAATCCGTG
    CATAAAGACA ATGGGAAAAC CATTGAGAAC GTGGAGGAGC ACAGCTATAA GCAAGGGAAA
    AAAATGCGTG ACACCCTAAG GACGACAGAA CGAGATCACA AGAAAAATGT GCAGTGCTCG
    TTTATGTTAG ACTCAGTTGG CGGGTCTCTG CCAAAAAAAA CAATTCCAGA TGTTGATCTC
    AATAAGCCTT ACCTCAGCCT TGGCTGTAGC AATGCTAAGC TTCCAGTCTC TGTGCCCATG
    CCAATCTCCA GAACTGCACG CCAGACTTCT AGGACTGATT GCCCAGCGGA CCGCTTAAAA
    TTCTTTGAAA CCCTGCGGCT TTTGTTAAAA CTTACCTCAG TCTCGAAAAA AAAGGACAGG
    GAGCAAAGAG GACAGGAAAG CACCTCTTCT GTCTGGCTGA ACCGATCCAA TGAACTGATC
    TGGCTAGAGC TGCAAGCTTG GCATGCTGGC CGGAGTATTA ATGACCAAGA CCTTTATCTC
    TATGCAGCCC GTCAAGCTAT CCCTGATATT ATCAATGAAA TCCTTACATT CAAAGTGAAC
    TATGGAAGCT TCTCCTATGT AAAAAATGGA GCCAGTCTCA ATGGTACGTC AGGAGAAGGA
    GTTTGCAAAG CAACACATGG AACCAGTGCT TTGGGTTACT CAAGTTACCA CGAACACCTC
    CATCGCCAGC GAATCTCATT TGAGCAAGTA AAGCGAATAA TGGAACTGCT AGAGTATATA
    GAAGCACTTT ATCCATCTCT GCAGGCTCTT CAAAAGGACT ATGAAAAGTA TGCTGCAAAA
    GACTTCCAGG ACAGAGTGCA GGCACTCTGT CTGTGGTTGA ACATTACAAA AGACTTAAAT
    CAAAAGCTAA GGATTATGGG GACTGTTTTG GGCATCAAAA ATCTCTCTGA CATTGGCTGG
    CCAGTGTTTG AAATTCCTTC TCCTCGACCG TCTAAGGACG ATGATCCAGA GGATGAAGGA
    GTTGATAGGG AAACTGAAGT AAAGGAATCC TCAGGAACAT GCACAGAGGA GAGCGATGGC
    GAAGAACAAA TCTCTGAGGA GAATGTTGAG GAACTTAGAC AAGCCATCAA GAACAATTTT
    ACTAATCAGC CAAATTTCGA CTTTCAGGAC CATTTTTCAA GGAAGCTTGA TAGGCTTGAA
    TCTGGGGATG ACTCTGCCTC TTGGATTATT CCAGAATGCA GTGCTGAGGC AGGCTGCAGC
    AAACACTGTT TGACTTCTAT TTACAGGCCG TTTGTAGACA AAGCACTGAA GCAAATGGGG
    TTGAGGAAGC TAATTTTAAG ACTACACAAG CTAATGCATG GTTCATTGCA AAGGGCTCGT
    ATGGCGTTGG TAAGGAGTGA TTGCCCGCTG GAGTTTTCAG AATGTCCAGA TCCCATGTTG
    TGTTCAGATT ACGTGCAGTT ATTAAATTCC CCACCTTGCT GTGAGCAAAA ATGCAGCACT
    GTATCATGGG AAGAGCTCAA ATCCATGGAT TTGCCATCTT TTGAACCTGC ATTCTTGGTC
    CTTTGCCGAG TCCTGCTCAA TGTTATCCAT GAATGTCTCA AGCTAAGGTT GGAACAAAGG
    CCTGCTGGGG AGCCATCTCT TTTGAGTATT AAACAGCTAG TGAGGGAGTG TAAGGAGGTT
    CTGAAGGGTG GCCTGTTAAT GAAGCAATAT TACCAATTTA TGTTGCGTGA GGTGTTAGAT
    GACTTGCAAA AGATTGATTG CAACATTGAT GCTTTTGAAG AGGACCTGCA TAAAATGTTG
    ATGGTTTACT TTGATTACAT GCGAAGCTGG ATCCAAATGC TACAGCAGTT ACCTCAAGCA
    TCTCATAGTT TAAAAAATCT CCTAGAGGAA GAATGGAATT TCACCAAAGA AATAACTCCA
    TATATAAGAG GAGGAGAAGC TCAAGCTGGA AAACTATTTT GTGATATTGC AGGAATGCTG
    CTGAAATCTA CAGGAATATT TTTAGATTCT GGACTACAAG ACAGTTGTGA TGAATTTTGG
    GCCAGTGCCG ATGACAGTAC TGCATCAGAT GAAATAAGGA GGTCTGTCAT AGAGACCAGC
    CGAGCACTCA AGGAACTGTT TCATGAAGCT AGAGAAAGGG CCTCCAAGGC TCTTGGATTT
    GCTAAAATGC TGAGGAAGGA CCTTGAAATA GCAGCAGAGT TTGCATTGTC AGCCCCTGTC
    CGAGATCTTC TGAATGCTCT GAAAACGAAA GAGTATGTGA AGGTACAAAT CCCTGGACTC
    GAGAATTTAC AAGTATTTGT CCCAAATAGT ATTGCAGGTG AAAAGTCTGT GATTTTGCAG
    CTCCTAAATG CGGCTGCTGG GAAAGATTGC TCAAAAGATT CAGATGAGGT TGCATATGAG
    GCCTATTTAA TAATGACCAA ACACAGTGAC AAAGACCATG AATTAGGTGA CATGTGGAGT
    GCATGGGAGG GCCAACCAGT CAAAGTGGTG CCACAAGTGG AAACTGTTGA TACGCTACGA
    AGCATGCAGG TTGACAACCT CCTTCTCGTA GTCATTCAGT CTGCCCACCT TGTGAGTCAG
    CGAAAAGCTT TCCAACAGTC TATAGAAGGG CTCATATCAC TCCACCAGGA ACAGACATCC
    AGCCAACCAG TCATTGCCAA AGCTCTGCAG CAACTAAAGA ATGATGCATT GCAGCTATGC
    AATAAGATAA GCAGTGCCAT TGACAGAGTT GATCATATGT TCACATCTGA GTTTGATGCT
    GAAGTTGATG AATCTGAATC TGCGACTTTG CAGCAATACT ACCGGGAAGC CATGATTCAG
    GCTTACAATT TTGGGTTTGA GTACCACAAA GAAGTTGTTC GTCTGATGTC TGGAGAATTC
    AGGCAGAAAA TTGGAGACAA ATATATCAGC TTTGCCAGAA AGTGGATGAA CTACGTGCTA
    ACAAAATGTG AGAGTGGCCG AGGCACTCGA CCCAGGTGGG CAACTCAAGG TTTTGATTTT
    CTTCAAGCCA TTGAACCAGC ATTTATTTCG GCTCTCCCAG AAGATGATTT CTTGAGTTTG
    CAAGCTCTGA TGAATGAGTG CATTGGCCAC GTTATTGGAA AACCTCACAG TCCTGTTACA
    GGTCTGTACC TTGCTATTCA TCGGAACAGT CCCCGTCCCG TGAAAGTACC TCGATGCCAT
    AGTGATCCAC CGAATCCTCA TCTTATCATC CCCACTCCTG AAGGCTTCAG CACACGGAGC
    GTCCCTTGCG ATGCACGGAA CCCTTGCAAC GTTACTGTCA GTCGCCCCGC TCCCATTGGT
    AGTGACTCAA CTCAACCTAA ATCTGTCAGC AGTGCAAATG ATACCAGGGG TTCCAGTGTC
    CCCGAAAATG ATCGACTGGC TTCAATAGCA GCCGAGTTGC AGTTCAGATC CCTTAGTCGT
    CACTCCAGTC CCACTGAGGA GCGAGAAGAA CCATCATATC CAAAAGGAGA TTCAAGTGGC
    TCATCCCGGC GGAGTTGGGA GCTCAGAACC TTAATCAACC AGACCAAAGA CACTGTTTCC
    AAACAAGCAC CAATAGAAGC TGTTCAGAAG TCTGTTCAGT TGTTTGAAGA AAGGAGATAC
    AGAGAGATGA GGAGGAAGAA TATCATTGGC CAAGTTTGCG ACACACCAAA ATCCTACGAT
    AATGTCATGC ATGTAGGGTT GAGAAAAGTG ACTTTCAAGT GGCAGAGAGG AAACAAAATT
    GGTGAAGGCC AATATGGGAA AGTATATACC TGCATTAGTG TTGACACTGG AGAGCTGATG
    GCTATGAAAG AAATAAGATT TCAACCTAAT GATCACAAAA CCATCAAAGA AACAGCTGAT
    GAACTGAAGA TTTTTGAAGG CATCAAACAC CCTAATCTTG TTCGGTATTT TGGTGTGGAG
    CTCCATAGGG AAGAGATGTA CATCTTCATG GAGTACTGTG ATGAGGGCAC GCTGGAGGAG
    GTGTCAAAAC TGGGCCTTCA GGAGCATGTC ATTAGGCTCT ACACAAAGCA GATCACCATT
    GCTATCAATG TACTACATGA ACACGGTATT GTTCATCGAG ACATTAAAGG AGCCAACATC
    TTTCTTACCT CATCCGGACT GATTAAACTG GGTGACTTTG GCTGCTCAGT AAAACTAAAG
    AACAACACTC AAACAATGCC TGGAGAAGTG AACAGTACAT TGGGTACTGC AGCGTACATG
    GCTCCAGAAG TAATTACTAG AGCAAAAGGA GAAGGGCATG GACGTGCAGC AGACATCTGG
    AGCCTGGGCT GTGTTGTTAT AGAGATGGTC ACTGGCAAGA GGCCTTGGCA CGAATATGAG
    CACAACTTCC AGATCATGTA TAGAGTGGGG ATGGGTCATA AACCTCCTAT TCCTGACAAA
    GTGAGTCCGG AGGGGAAAGA CTTCCTTTGC CACTGCCTGG AAAGTGACCC AAAGATGAGG
    TGGACAGCCA GCCAGCTCCT CGACCATCCT TTTGTCAAGG TTTGCACAGA TGAAGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33777
    Clone ID Related Accession (Same CDS sequence) XM_015284321.2 , XM_015284321.1 , XM_025148975.1
    Accession Version XM_015284321.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4680bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product mitogen-activated protein kinase kinase kinase 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455837.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAATCTGC ATATTTCCAG GAAAGAGTGC AAATCAGAAG ATGCTACTTG CAAGATCTCT 
    GAATCTGAAC CTGAGGAGTT ATCAGATGAA GCAAGTGCTG ACACCTTCTA TGGAACTTCT
    CCTCCTAGTA CACCCCGCCA GATGAAGCGT ATGTCAACAA AGCATCAGAA AAACAACGTC
    GGGAGACCTG CTAGCCGCTC TACTTTGAAA GAAAAGATGA GTATACCACC TCAATCCGTG
    CATAAAGACA ATGGGAAAAC CATTGAGAAC GTGGAGGAGC ACAGCTATAA GCAAGGGAAA
    AAAATGCGTG ACACCCTAAG GACGACAGAA CGAGATCACA AGAAAAATGT GCAGTGCTCG
    TTTATGTTAG ACTCAGTTGG CGGGTCTCTG CCAAAAAAAA CAATTCCAGA TGTTGATCTC
    AATAAGCCTT ACCTCAGCCT TGGCTGTAGC AATGCTAAGC TTCCAGTCTC TGTGCCCATG
    CCAATCTCCA GAACTGCACG CCAGACTTCT AGGACTGATT GCCCAGCGGA CCGCTTAAAA
    TTCTTTGAAA CCCTGCGGCT TTTGTTAAAA CTTACCTCAG TCTCGAAAAA AAAGGACAGG
    GAGCAAAGAG GACAGGAAAG CACCTCTTCT GTCTGGCTGA ACCGATCCAA TGAACTGATC
    TGGCTAGAGC TGCAAGCTTG GCATGCTGGC CGGAGTATTA ATGACCAAGA CCTTTATCTC
    TATGCAGCCC GTCAAGCTAT CCCTGATATT ATCAATGAAA TCCTTACATT CAAAGTGAAC
    TATGGAAGCT TCTCCTATGT AAAAAATGGA GCCAGTCTCA ATGGTACGTC AGGAGAAGGA
    GTTTGCAAAG CAACACATGG AACCAGTGCT TTGGGTTACT CAAGTTACCA CGAACACCTC
    CATCGCCAGC GAATCTCATT TGAGCAAGTA AAGCGAATAA TGGAACTGCT AGAGTATATA
    GAAGCACTTT ATCCATCTCT GCAGGCTCTT CAAAAGGACT ATGAAAAGTA TGCTGCAAAA
    GACTTCCAGG ACAGAGTGCA GGCACTCTGT CTGTGGTTGA ACATTACAAA AGACTTAAAT
    CAAAAGCTAA GGATTATGGG GACTGTTTTG GGCATCAAAA ATCTCTCTGA CATTGGCTGG
    CCAGTGTTTG AAATTCCTTC TCCTCGACCG TCTAAGGACG ATGATCCAGA GGATGAAGGA
    GTTGATAGGG AAACTGAAGT AAAGGAATCC TCAGGAACAT GCACAGAGGA GAGCGATGGC
    GAAGAACAAA TCTCTGAGGA GAATGTTGAG GAACTTAGAC AAGCCATCAA GAACAATTTT
    ACTAATCAGC CAAATTTCGA CTTTCAGGAC CATTTTTCAA GGAAGCTTGA TAGGCTTGAA
    TCTGGGGATG ACTCTGCCTC TTGGATTATT CCAGAATGCA GTGCTGAGGC AGGCTGCAGC
    AAACACTGTT TGACTTCTAT TTACAGGCCG TTTGTAGACA AAGCACTGAA GCAAATGGGG
    TTGAGGAAGC TAATTTTAAG ACTACACAAG CTAATGCATG GTTCATTGCA AAGGGCTCGT
    ATGGCGTTGG TAAGGAGTGA TTGCCCGCTG GAGTTTTCAG AATGTCCAGA TCCCATGTTG
    TGTTCAGATT ACGTGCAGTT ATTAAATTCC CCACCTTGCT GTGAGCAAAA ATGCAGCACT
    GTATCATGGG AAGAGCTCAA ATCCATGGAT TTGCCATCTT TTGAACCTGC ATTCTTGGTC
    CTTTGCCGAG TCCTGCTCAA TGTTATCCAT GAATGTCTCA AGCTAAGGTT GGAACAAAGG
    CCTGCTGGGG AGCCATCTCT TTTGAGTATT AAACAGCTAG TGAGGGAGTG TAAGGAGGTT
    CTGAAGGGTG GCCTGTTAAT GAAGCAATAT TACCAATTTA TGTTGCGTGA GGTGTTAGAT
    GACTTGCAAA AGATTGATTG CAACATTGAT GCTTTTGAAG AGGACCTGCA TAAAATGTTG
    ATGGTTTACT TTGATTACAT GCGAAGCTGG ATCCAAATGC TACAGCAGTT ACCTCAAGCA
    TCTCATAGTT TAAAAAATCT CCTAGAGGAA GAATGGAATT TCACCAAAGA AATAACTCCA
    TATATAAGAG GAGGAGAAGC TCAAGCTGGA AAACTATTTT GTGATATTGC AGGAATGCTG
    CTGAAATCTA CAGGAATATT TTTAGATTCT GGACTACAAG ACAGTTGTGA TGAATTTTGG
    GCCAGTGCCG ATGACAGTAC TGCATCAGAT GAAATAAGGA GGTCTGTCAT AGAGACCAGC
    CGAGCACTCA AGGAACTGTT TCATGAAGCT AGAGAAAGGG CCTCCAAGGC TCTTGGATTT
    GCTAAAATGC TGAGGAAGGA CCTTGAAATA GCAGCAGAGT TTGCATTGTC AGCCCCTGTC
    CGAGATCTTC TGAATGCTCT GAAAACGAAA GAGTATGTGA AGGTACAAAT CCCTGGACTC
    GAGAATTTAC AAGTATTTGT CCCAAATAGT ATTGCAGGTG AAAAGTCTGT GATTTTGCAG
    CTCCTAAATG CGGCTGCTGG GAAAGATTGC TCAAAAGATT CAGATGAGGT TGCATATGAG
    GCCTATTTAA TAATGACCAA ACACAGTGAC AAAGACCATG AATTAGGTGA CATGTGGAGT
    GCATGGGAGG GCCAACCAGT CAAAGTGGTG CCACAAGTGG AAACTGTTGA TACGCTACGA
    AGCATGCAGG TTGACAACCT CCTTCTCGTA GTCATTCAGT CTGCCCACCT TGTGAGTCAG
    CGAAAAGCTT TCCAACAGTC TATAGAAGGG CTCATATCAC TCCACCAGGA ACAGACATCC
    AGCCAACCAG TCATTGCCAA AGCTCTGCAG CAACTAAAGA ATGATGCATT GCAGCTATGC
    AATAAGATAA GCAGTGCCAT TGACAGAGTT GATCATATGT TCACATCTGA GTTTGATGCT
    GAAGTTGATG AATCTGAATC TGCGACTTTG CAGCAATACT ACCGGGAAGC CATGATTCAG
    GCTTACAATT TTGGGTTTGA GTACCACAAA GAAGTTGTTC GTCTGATGTC TGGAGAATTC
    AGGCAGAAAA TTGGAGACAA ATATATCAGC TTTGCCAGAA AGTGGATGAA CTACGTGCTA
    ACAAAATGTG AGAGTGGCCG AGGCACTCGA CCCAGGTGGG CAACTCAAGG TTTTGATTTT
    CTTCAAGCCA TTGAACCAGC ATTTATTTCG GCTCTCCCAG AAGATGATTT CTTGAGTTTG
    CAAGCTCTGA TGAATGAGTG CATTGGCCAC GTTATTGGAA AACCTCACAG TCCTGTTACA
    GGTCTGTACC TTGCTATTCA TCGGAACAGT CCCCGTCCCG TGAAAGTACC TCGATGCCAT
    AGTGATCCAC CGAATCCTCA TCTTATCATC CCCACTCCTG AAGGCTTCAG CACACGGAGC
    GTCCCTTGCG ATGCACGGAA CCCTTGCAAC GTTACTGTCA GTCGCCCCGC TCCCATTGGT
    AGTGACTCAA CTCAACCTAA ATCTGTCAGC AGTGCAAATG ATACCAGGGG TTCCAGTGTC
    CCCGAAAATG ATCGACTGGC TTCAATAGCA GCCGAGTTGC AGTTCAGATC CCTTAGTCGT
    CACTCCAGTC CCACTGAGGA GCGAGAAGAA CCATCATATC CAAAAGGAGA TTCAAGTGGC
    TCATCCCGGC GGAGTTGGGA GCTCAGAACC TTAATCAACC AGACCAAAGA CACTGTTTCC
    AAACAAGCAC CAATAGAAGC TGTTCAGAAG TCTGTTCAGT TGTTTGAAGA AAGGAGATAC
    AGAGAGATGA GGAGGAAGAA TATCATTGGC CAAGTTTGCG ACACACCAAA ATCCTACGAT
    AATGTCATGC ATGTAGGGTT GAGAAAAGTG ACTTTCAAGT GGCAGAGAGG AAACAAAATT
    GGTGAAGGCC AATATGGGAA AGTATATACC TGCATTAGTG TTGACACTGG AGAGCTGATG
    GCTATGAAAG AAATAAGATT TCAACCTAAT GATCACAAAA CCATCAAAGA AACAGCTGAT
    GAACTGAAGA TTTTTGAAGG CATCAAACAC CCTAATCTTG TTCGGTATTT TGGTGTGGAG
    CTCCATAGGG AAGAGATGTA CATCTTCATG GAGTACTGTG ATGAGGGCAC GCTGGAGGAG
    GTGTCAAAAC TGGGCCTTCA GGAGCATGTC ATTAGGCTCT ACACAAAGCA GATCACCATT
    GCTATCAATG TACTACATGA ACACGGTATT GTTCATCGAG ACATTAAAGG AGCCAACATC
    TTTCTTACCT CATCCGGACT GATTAAACTG GGTGACTTTG GCTGCTCAGT AAAACTAAAG
    AACAACACTC AAACAATGCC TGGAGAAGTG AACAGTACAT TGGGTACTGC AGCGTACATG
    GCTCCAGAAG TAATTACTAG AGCAAAAGGA GAAGGGCATG GACGTGCAGC AGACATCTGG
    AGCCTGGGCT GTGTTGTTAT AGAGATGGTC ACTGGCAAGA GGCCTTGGCA CGAATATGAG
    CACAACTTCC AGATCATGTA TAGAGTGGGG ATGGGTCATA AACCTCCTAT TCCTGACAAA
    GTGAGTCCGG AGGGGAAAGA CTTCCTTTGC CACTGCCTGG AAAGTGACCC AAAGATGAGG
    TGGACAGCCA GCCAGCTCCT CGACCATCCT TTTGTCAAGG TTTGCACAGA TGAAGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139807.1
    CDS388..5067
    Translation

    Target ORF information:

    RefSeq Version XM_015284321.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284321.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAATCTGC ATATTTCCAG GAAAGAGTGC AAATCAGAAG ATGCTACTTG CAAGATCTCT 
    GAATCTGAAC CTGAGGAGTT ATCAGATGAA GCAAGTGCTG ACACCTTCTA TGGAACTTCT
    CCTCCTAGTA CACCCCGCCA GATGAAGCGT ATGTCAACAA AGCATCAGAA AAACAACGTC
    GGGAGACCTG CTAGCCGCTC TACTTTGAAA GAAAAGATGA GTATACCACC TCAATCCGTG
    CATAAAGACA ATGGGAAAAC CATTGAGAAC GTGGAGGAGC ACAGCTATAA GCAAGGGAAA
    AAAATGCGTG ACACCCTAAG GACGACAGAA CGAGATCACA AGAAAAATGT GCAGTGCTCG
    TTTATGTTAG ACTCAGTTGG CGGGTCTCTG CCAAAAAAAA CAATTCCAGA TGTTGATCTC
    AATAAGCCTT ACCTCAGCCT TGGCTGTAGC AATGCTAAGC TTCCAGTCTC TGTGCCCATG
    CCAATCTCCA GAACTGCACG CCAGACTTCT AGGACTGATT GCCCAGCGGA CCGCTTAAAA
    TTCTTTGAAA CCCTGCGGCT TTTGTTAAAA CTTACCTCAG TCTCGAAAAA AAAGGACAGG
    GAGCAAAGAG GACAGGAAAG CACCTCTTCT GTCTGGCTGA ACCGATCCAA TGAACTGATC
    TGGCTAGAGC TGCAAGCTTG GCATGCTGGC CGGAGTATTA ATGACCAAGA CCTTTATCTC
    TATGCAGCCC GTCAAGCTAT CCCTGATATT ATCAATGAAA TCCTTACATT CAAAGTGAAC
    TATGGAAGCT TCTCCTATGT AAAAAATGGA GCCAGTCTCA ATGGTACGTC AGGAGAAGGA
    GTTTGCAAAG CAACACATGG AACCAGTGCT TTGGGTTACT CAAGTTACCA CGAACACCTC
    CATCGCCAGC GAATCTCATT TGAGCAAGTA AAGCGAATAA TGGAACTGCT AGAGTATATA
    GAAGCACTTT ATCCATCTCT GCAGGCTCTT CAAAAGGACT ATGAAAAGTA TGCTGCAAAA
    GACTTCCAGG ACAGAGTGCA GGCACTCTGT CTGTGGTTGA ACATTACAAA AGACTTAAAT
    CAAAAGCTAA GGATTATGGG GACTGTTTTG GGCATCAAAA ATCTCTCTGA CATTGGCTGG
    CCAGTGTTTG AAATTCCTTC TCCTCGACCG TCTAAGGACG ATGATCCAGA GGATGAAGGA
    GTTGATAGGG AAACTGAAGT AAAGGAATCC TCAGGAACAT GCACAGAGGA GAGCGATGGC
    GAAGAACAAA TCTCTGAGGA GAATGTTGAG GAACTTAGAC AAGCCATCAA GAACAATTTT
    ACTAATCAGC CAAATTTCGA CTTTCAGGAC CATTTTTCAA GGAAGCTTGA TAGGCTTGAA
    TCTGGGGATG ACTCTGCCTC TTGGATTATT CCAGAATGCA GTGCTGAGGC AGGCTGCAGC
    AAACACTGTT TGACTTCTAT TTACAGGCCG TTTGTAGACA AAGCACTGAA GCAAATGGGG
    TTGAGGAAGC TAATTTTAAG ACTACACAAG CTAATGCATG GTTCATTGCA AAGGGCTCGT
    ATGGCGTTGG TAAGGAGTGA TTGCCCGCTG GAGTTTTCAG AATGTCCAGA TCCCATGTTG
    TGTTCAGATT ACGTGCAGTT ATTAAATTCC CCACCTTGCT GTGAGCAAAA ATGCAGCACT
    GTATCATGGG AAGAGCTCAA ATCCATGGAT TTGCCATCTT TTGAACCTGC ATTCTTGGTC
    CTTTGCCGAG TCCTGCTCAA TGTTATCCAT GAATGTCTCA AGCTAAGGTT GGAACAAAGG
    CCTGCTGGGG AGCCATCTCT TTTGAGTATT AAACAGCTAG TGAGGGAGTG TAAGGAGGTT
    CTGAAGGGTG GCCTGTTAAT GAAGCAATAT TACCAATTTA TGTTGCGTGA GGTGTTAGAT
    GACTTGCAAA AGATTGATTG CAACATTGAT GCTTTTGAAG AGGACCTGCA TAAAATGTTG
    ATGGTTTACT TTGATTACAT GCGAAGCTGG ATCCAAATGC TACAGCAGTT ACCTCAAGCA
    TCTCATAGTT TAAAAAATCT CCTAGAGGAA GAATGGAATT TCACCAAAGA AATAACTCCA
    TATATAAGAG GAGGAGAAGC TCAAGCTGGA AAACTATTTT GTGATATTGC AGGAATGCTG
    CTGAAATCTA CAGGAATATT TTTAGATTCT GGACTACAAG ACAGTTGTGA TGAATTTTGG
    GCCAGTGCCG ATGACAGTAC TGCATCAGAT GAAATAAGGA GGTCTGTCAT AGAGACCAGC
    CGAGCACTCA AGGAACTGTT TCATGAAGCT AGAGAAAGGG CCTCCAAGGC TCTTGGATTT
    GCTAAAATGC TGAGGAAGGA CCTTGAAATA GCAGCAGAGT TTGCATTGTC AGCCCCTGTC
    CGAGATCTTC TGAATGCTCT GAAAACGAAA GAGTATGTGA AGGTACAAAT CCCTGGACTC
    GAGAATTTAC AAGTATTTGT CCCAAATAGT ATTGCAGGTG AAAAGTCTGT GATTTTGCAG
    CTCCTAAATG CGGCTGCTGG GAAAGATTGC TCAAAAGATT CAGATGAGGT TGCATATGAG
    GCCTATTTAA TAATGACCAA ACACAGTGAC AAAGACCATG AATTAGGTGA CATGTGGAGT
    GCATGGGAGG GCCAACCAGT CAAAGTGGTG CCACAAGTGG AAACTGTTGA TACGCTACGA
    AGCATGCAGG TTGACAACCT CCTTCTCGTA GTCATTCAGT CTGCCCACCT TGTGAGTCAG
    CGAAAAGCTT TCCAACAGTC TATAGAAGGG CTCATATCAC TCCACCAGGA ACAGACATCC
    AGCCAACCAG TCATTGCCAA AGCTCTGCAG CAACTAAAGA ATGATGCATT GCAGCTATGC
    AATAAGATAA GCAGTGCCAT TGACAGAGTT GATCATATGT TCACATCTGA GTTTGATGCT
    GAAGTTGATG AATCTGAATC TGCGACTTTG CAGCAATACT ACCGGGAAGC CATGATTCAG
    GCTTACAATT TTGGGTTTGA GTACCACAAA GAAGTTGTTC GTCTGATGTC TGGAGAATTC
    AGGCAGAAAA TTGGAGACAA ATATATCAGC TTTGCCAGAA AGTGGATGAA CTACGTGCTA
    ACAAAATGTG AGAGTGGCCG AGGCACTCGA CCCAGGTGGG CAACTCAAGG TTTTGATTTT
    CTTCAAGCCA TTGAACCAGC ATTTATTTCG GCTCTCCCAG AAGATGATTT CTTGAGTTTG
    CAAGCTCTGA TGAATGAGTG CATTGGCCAC GTTATTGGAA AACCTCACAG TCCTGTTACA
    GGTCTGTACC TTGCTATTCA TCGGAACAGT CCCCGTCCCG TGAAAGTACC TCGATGCCAT
    AGTGATCCAC CGAATCCTCA TCTTATCATC CCCACTCCTG AAGGCTTCAG CACACGGAGC
    GTCCCTTGCG ATGCACGGAA CCCTTGCAAC GTTACTGTCA GTCGCCCCGC TCCCATTGGT
    AGTGACTCAA CTCAACCTAA ATCTGTCAGC AGTGCAAATG ATACCAGGGG TTCCAGTGTC
    CCCGAAAATG ATCGACTGGC TTCAATAGCA GCCGAGTTGC AGTTCAGATC CCTTAGTCGT
    CACTCCAGTC CCACTGAGGA GCGAGAAGAA CCATCATATC CAAAAGGAGA TTCAAGTGGC
    TCATCCCGGC GGAGTTGGGA GCTCAGAACC TTAATCAACC AGACCAAAGA CACTGTTTCC
    AAACAAGCAC CAATAGAAGC TGTTCAGAAG TCTGTTCAGT TGTTTGAAGA AAGGAGATAC
    AGAGAGATGA GGAGGAAGAA TATCATTGGC CAAGTTTGCG ACACACCAAA ATCCTACGAT
    AATGTCATGC ATGTAGGGTT GAGAAAAGTG ACTTTCAAGT GGCAGAGAGG AAACAAAATT
    GGTGAAGGCC AATATGGGAA AGTATATACC TGCATTAGTG TTGACACTGG AGAGCTGATG
    GCTATGAAAG AAATAAGATT TCAACCTAAT GATCACAAAA CCATCAAAGA AACAGCTGAT
    GAACTGAAGA TTTTTGAAGG CATCAAACAC CCTAATCTTG TTCGGTATTT TGGTGTGGAG
    CTCCATAGGG AAGAGATGTA CATCTTCATG GAGTACTGTG ATGAGGGCAC GCTGGAGGAG
    GTGTCAAAAC TGGGCCTTCA GGAGCATGTC ATTAGGCTCT ACACAAAGCA GATCACCATT
    GCTATCAATG TACTACATGA ACACGGTATT GTTCATCGAG ACATTAAAGG AGCCAACATC
    TTTCTTACCT CATCCGGACT GATTAAACTG GGTGACTTTG GCTGCTCAGT AAAACTAAAG
    AACAACACTC AAACAATGCC TGGAGAAGTG AACAGTACAT TGGGTACTGC AGCGTACATG
    GCTCCAGAAG TAATTACTAG AGCAAAAGGA GAAGGGCATG GACGTGCAGC AGACATCTGG
    AGCCTGGGCT GTGTTGTTAT AGAGATGGTC ACTGGCAAGA GGCCTTGGCA CGAATATGAG
    CACAACTTCC AGATCATGTA TAGAGTGGGG ATGGGTCATA AACCTCCTAT TCCTGACAAA
    GTGAGTCCGG AGGGGAAAGA CTTCCTTTGC CACTGCCTGG AAAGTGACCC AAAGATGAGG
    TGGACAGCCA GCCAGCTCCT CGACCATCCT TTTGTCAAGG TTTGCACAGA TGAAGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33777
    Clone ID Related Accession (Same CDS sequence) XM_015284321.2 , XM_015284321.1 , XM_025148975.1
    Accession Version XM_025148975.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4680bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product mitogen-activated protein kinase kinase kinase 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAATCTGC ATATTTCCAG GAAAGAGTGC AAATCAGAAG ATGCTACTTG CAAGATCTCT 
    GAATCTGAAC CTGAGGAGTT ATCAGATGAA GCAAGTGCTG ACACCTTCTA TGGAACTTCT
    CCTCCTAGTA CACCCCGCCA GATGAAGCGT ATGTCAACAA AGCATCAGAA AAACAACGTC
    GGGAGACCTG CTAGCCGCTC TACTTTGAAA GAAAAGATGA GTATACCACC TCAATCCGTG
    CATAAAGACA ATGGGAAAAC CATTGAGAAC GTGGAGGAGC ACAGCTATAA GCAAGGGAAA
    AAAATGCGTG ACACCCTAAG GACGACAGAA CGAGATCACA AGAAAAATGT GCAGTGCTCG
    TTTATGTTAG ACTCAGTTGG CGGGTCTCTG CCAAAAAAAA CAATTCCAGA TGTTGATCTC
    AATAAGCCTT ACCTCAGCCT TGGCTGTAGC AATGCTAAGC TTCCAGTCTC TGTGCCCATG
    CCAATCTCCA GAACTGCACG CCAGACTTCT AGGACTGATT GCCCAGCGGA CCGCTTAAAA
    TTCTTTGAAA CCCTGCGGCT TTTGTTAAAA CTTACCTCAG TCTCGAAAAA AAAGGACAGG
    GAGCAAAGAG GACAGGAAAG CACCTCTTCT GTCTGGCTGA ACCGATCCAA TGAACTGATC
    TGGCTAGAGC TGCAAGCTTG GCATGCTGGC CGGAGTATTA ATGACCAAGA CCTTTATCTC
    TATGCAGCCC GTCAAGCTAT CCCTGATATT ATCAATGAAA TCCTTACATT CAAAGTGAAC
    TATGGAAGCT TCTCCTATGT AAAAAATGGA GCCAGTCTCA ATGGTACGTC AGGAGAAGGA
    GTTTGCAAAG CAACACATGG AACCAGTGCT TTGGGTTACT CAAGTTACCA CGAACACCTC
    CATCGCCAGC GAATCTCATT TGAGCAAGTA AAGCGAATAA TGGAACTGCT AGAGTATATA
    GAAGCACTTT ATCCATCTCT GCAGGCTCTT CAAAAGGACT ATGAAAAGTA TGCTGCAAAA
    GACTTCCAGG ACAGAGTGCA GGCACTCTGT CTGTGGTTGA ACATTACAAA AGACTTAAAT
    CAAAAGCTAA GGATTATGGG GACTGTTTTG GGCATCAAAA ATCTCTCTGA CATTGGCTGG
    CCAGTGTTTG AAATTCCTTC TCCTCGACCG TCTAAGGACG ATGATCCAGA GGATGAAGGA
    GTTGATAGGG AAACTGAAGT AAAGGAATCC TCAGGAACAT GCACAGAGGA GAGCGATGGC
    GAAGAACAAA TCTCTGAGGA GAATGTTGAG GAACTTAGAC AAGCCATCAA GAACAATTTT
    ACTAATCAGC CAAATTTCGA CTTTCAGGAC CATTTTTCAA GGAAGCTTGA TAGGCTTGAA
    TCTGGGGATG ACTCTGCCTC TTGGATTATT CCAGAATGCA GTGCTGAGGC AGGCTGCAGC
    AAACACTGTT TGACTTCTAT TTACAGGCCG TTTGTAGACA AAGCACTGAA GCAAATGGGG
    TTGAGGAAGC TAATTTTAAG ACTACACAAG CTAATGCATG GTTCATTGCA AAGGGCTCGT
    ATGGCGTTGG TAAGGAGTGA TTGCCCGCTG GAGTTTTCAG AATGTCCAGA TCCCATGTTG
    TGTTCAGATT ACGTGCAGTT ATTAAATTCC CCACCTTGCT GTGAGCAAAA ATGCAGCACT
    GTATCATGGG AAGAGCTCAA ATCCATGGAT TTGCCATCTT TTGAACCTGC ATTCTTGGTC
    CTTTGCCGAG TCCTGCTCAA TGTTATCCAT GAATGTCTCA AGCTAAGGTT GGAACAAAGG
    CCTGCTGGGG AGCCATCTCT TTTGAGTATT AAACAGCTAG TGAGGGAGTG TAAGGAGGTT
    CTGAAGGGTG GCCTGTTAAT GAAGCAATAT TACCAATTTA TGTTGCGTGA GGTGTTAGAT
    GACTTGCAAA AGATTGATTG CAACATTGAT GCTTTTGAAG AGGACCTGCA TAAAATGTTG
    ATGGTTTACT TTGATTACAT GCGAAGCTGG ATCCAAATGC TACAGCAGTT ACCTCAAGCA
    TCTCATAGTT TAAAAAATCT CCTAGAGGAA GAATGGAATT TCACCAAAGA AATAACTCCA
    TATATAAGAG GAGGAGAAGC TCAAGCTGGA AAACTATTTT GTGATATTGC AGGAATGCTG
    CTGAAATCTA CAGGAATATT TTTAGATTCT GGACTACAAG ACAGTTGTGA TGAATTTTGG
    GCCAGTGCCG ATGACAGTAC TGCATCAGAT GAAATAAGGA GGTCTGTCAT AGAGACCAGC
    CGAGCACTCA AGGAACTGTT TCATGAAGCT AGAGAAAGGG CCTCCAAGGC TCTTGGATTT
    GCTAAAATGC TGAGGAAGGA CCTTGAAATA GCAGCAGAGT TTGCATTGTC AGCCCCTGTC
    CGAGATCTTC TGAATGCTCT GAAAACGAAA GAGTATGTGA AGGTACAAAT CCCTGGACTC
    GAGAATTTAC AAGTATTTGT CCCAAATAGT ATTGCAGGTG AAAAGTCTGT GATTTTGCAG
    CTCCTAAATG CGGCTGCTGG GAAAGATTGC TCAAAAGATT CAGATGAGGT TGCATATGAG
    GCCTATTTAA TAATGACCAA ACACAGTGAC AAAGACCATG AATTAGGTGA CATGTGGAGT
    GCATGGGAGG GCCAACCAGT CAAAGTGGTG CCACAAGTGG AAACTGTTGA TACGCTACGA
    AGCATGCAGG TTGACAACCT CCTTCTCGTA GTCATTCAGT CTGCCCACCT TGTGAGTCAG
    CGAAAAGCTT TCCAACAGTC TATAGAAGGG CTCATATCAC TCCACCAGGA ACAGACATCC
    AGCCAACCAG TCATTGCCAA AGCTCTGCAG CAACTAAAGA ATGATGCATT GCAGCTATGC
    AATAAGATAA GCAGTGCCAT TGACAGAGTT GATCATATGT TCACATCTGA GTTTGATGCT
    GAAGTTGATG AATCTGAATC TGCGACTTTG CAGCAATACT ACCGGGAAGC CATGATTCAG
    GCTTACAATT TTGGGTTTGA GTACCACAAA GAAGTTGTTC GTCTGATGTC TGGAGAATTC
    AGGCAGAAAA TTGGAGACAA ATATATCAGC TTTGCCAGAA AGTGGATGAA CTACGTGCTA
    ACAAAATGTG AGAGTGGCCG AGGCACTCGA CCCAGGTGGG CAACTCAAGG TTTTGATTTT
    CTTCAAGCCA TTGAACCAGC ATTTATTTCG GCTCTCCCAG AAGATGATTT CTTGAGTTTG
    CAAGCTCTGA TGAATGAGTG CATTGGCCAC GTTATTGGAA AACCTCACAG TCCTGTTACA
    GGTCTGTACC TTGCTATTCA TCGGAACAGT CCCCGTCCCG TGAAAGTACC TCGATGCCAT
    AGTGATCCAC CGAATCCTCA TCTTATCATC CCCACTCCTG AAGGCTTCAG CACACGGAGC
    GTCCCTTGCG ATGCACGGAA CCCTTGCAAC GTTACTGTCA GTCGCCCCGC TCCCATTGGT
    AGTGACTCAA CTCAACCTAA ATCTGTCAGC AGTGCAAATG ATACCAGGGG TTCCAGTGTC
    CCCGAAAATG ATCGACTGGC TTCAATAGCA GCCGAGTTGC AGTTCAGATC CCTTAGTCGT
    CACTCCAGTC CCACTGAGGA GCGAGAAGAA CCATCATATC CAAAAGGAGA TTCAAGTGGC
    TCATCCCGGC GGAGTTGGGA GCTCAGAACC TTAATCAACC AGACCAAAGA CACTGTTTCC
    AAACAAGCAC CAATAGAAGC TGTTCAGAAG TCTGTTCAGT TGTTTGAAGA AAGGAGATAC
    AGAGAGATGA GGAGGAAGAA TATCATTGGC CAAGTTTGCG ACACACCAAA ATCCTACGAT
    AATGTCATGC ATGTAGGGTT GAGAAAAGTG ACTTTCAAGT GGCAGAGAGG AAACAAAATT
    GGTGAAGGCC AATATGGGAA AGTATATACC TGCATTAGTG TTGACACTGG AGAGCTGATG
    GCTATGAAAG AAATAAGATT TCAACCTAAT GATCACAAAA CCATCAAAGA AACAGCTGAT
    GAACTGAAGA TTTTTGAAGG CATCAAACAC CCTAATCTTG TTCGGTATTT TGGTGTGGAG
    CTCCATAGGG AAGAGATGTA CATCTTCATG GAGTACTGTG ATGAGGGCAC GCTGGAGGAG
    GTGTCAAAAC TGGGCCTTCA GGAGCATGTC ATTAGGCTCT ACACAAAGCA GATCACCATT
    GCTATCAATG TACTACATGA ACACGGTATT GTTCATCGAG ACATTAAAGG AGCCAACATC
    TTTCTTACCT CATCCGGACT GATTAAACTG GGTGACTTTG GCTGCTCAGT AAAACTAAAG
    AACAACACTC AAACAATGCC TGGAGAAGTG AACAGTACAT TGGGTACTGC AGCGTACATG
    GCTCCAGAAG TAATTACTAG AGCAAAAGGA GAAGGGCATG GACGTGCAGC AGACATCTGG
    AGCCTGGGCT GTGTTGTTAT AGAGATGGTC ACTGGCAAGA GGCCTTGGCA CGAATATGAG
    CACAACTTCC AGATCATGTA TAGAGTGGGG ATGGGTCATA AACCTCCTAT TCCTGACAAA
    GTGAGTCCGG AGGGGAAAGA CTTCCTTTGC CACTGCCTGG AAAGTGACCC AAAGATGAGG
    TGGACAGCCA GCCAGCTCCT CGACCATCCT TTTGTCAAGG TTTGCACAGA TGAAGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025004743.1
    CDS470..5149
    Translation

    Target ORF information:

    RefSeq Version XM_025148975.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025148975.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAATCTGC ATATTTCCAG GAAAGAGTGC AAATCAGAAG ATGCTACTTG CAAGATCTCT 
    GAATCTGAAC CTGAGGAGTT ATCAGATGAA GCAAGTGCTG ACACCTTCTA TGGAACTTCT
    CCTCCTAGTA CACCCCGCCA GATGAAGCGT ATGTCAACAA AGCATCAGAA AAACAACGTC
    GGGAGACCTG CTAGCCGCTC TACTTTGAAA GAAAAGATGA GTATACCACC TCAATCCGTG
    CATAAAGACA ATGGGAAAAC CATTGAGAAC GTGGAGGAGC ACAGCTATAA GCAAGGGAAA
    AAAATGCGTG ACACCCTAAG GACGACAGAA CGAGATCACA AGAAAAATGT GCAGTGCTCG
    TTTATGTTAG ACTCAGTTGG CGGGTCTCTG CCAAAAAAAA CAATTCCAGA TGTTGATCTC
    AATAAGCCTT ACCTCAGCCT TGGCTGTAGC AATGCTAAGC TTCCAGTCTC TGTGCCCATG
    CCAATCTCCA GAACTGCACG CCAGACTTCT AGGACTGATT GCCCAGCGGA CCGCTTAAAA
    TTCTTTGAAA CCCTGCGGCT TTTGTTAAAA CTTACCTCAG TCTCGAAAAA AAAGGACAGG
    GAGCAAAGAG GACAGGAAAG CACCTCTTCT GTCTGGCTGA ACCGATCCAA TGAACTGATC
    TGGCTAGAGC TGCAAGCTTG GCATGCTGGC CGGAGTATTA ATGACCAAGA CCTTTATCTC
    TATGCAGCCC GTCAAGCTAT CCCTGATATT ATCAATGAAA TCCTTACATT CAAAGTGAAC
    TATGGAAGCT TCTCCTATGT AAAAAATGGA GCCAGTCTCA ATGGTACGTC AGGAGAAGGA
    GTTTGCAAAG CAACACATGG AACCAGTGCT TTGGGTTACT CAAGTTACCA CGAACACCTC
    CATCGCCAGC GAATCTCATT TGAGCAAGTA AAGCGAATAA TGGAACTGCT AGAGTATATA
    GAAGCACTTT ATCCATCTCT GCAGGCTCTT CAAAAGGACT ATGAAAAGTA TGCTGCAAAA
    GACTTCCAGG ACAGAGTGCA GGCACTCTGT CTGTGGTTGA ACATTACAAA AGACTTAAAT
    CAAAAGCTAA GGATTATGGG GACTGTTTTG GGCATCAAAA ATCTCTCTGA CATTGGCTGG
    CCAGTGTTTG AAATTCCTTC TCCTCGACCG TCTAAGGACG ATGATCCAGA GGATGAAGGA
    GTTGATAGGG AAACTGAAGT AAAGGAATCC TCAGGAACAT GCACAGAGGA GAGCGATGGC
    GAAGAACAAA TCTCTGAGGA GAATGTTGAG GAACTTAGAC AAGCCATCAA GAACAATTTT
    ACTAATCAGC CAAATTTCGA CTTTCAGGAC CATTTTTCAA GGAAGCTTGA TAGGCTTGAA
    TCTGGGGATG ACTCTGCCTC TTGGATTATT CCAGAATGCA GTGCTGAGGC AGGCTGCAGC
    AAACACTGTT TGACTTCTAT TTACAGGCCG TTTGTAGACA AAGCACTGAA GCAAATGGGG
    TTGAGGAAGC TAATTTTAAG ACTACACAAG CTAATGCATG GTTCATTGCA AAGGGCTCGT
    ATGGCGTTGG TAAGGAGTGA TTGCCCGCTG GAGTTTTCAG AATGTCCAGA TCCCATGTTG
    TGTTCAGATT ACGTGCAGTT ATTAAATTCC CCACCTTGCT GTGAGCAAAA ATGCAGCACT
    GTATCATGGG AAGAGCTCAA ATCCATGGAT TTGCCATCTT TTGAACCTGC ATTCTTGGTC
    CTTTGCCGAG TCCTGCTCAA TGTTATCCAT GAATGTCTCA AGCTAAGGTT GGAACAAAGG
    CCTGCTGGGG AGCCATCTCT TTTGAGTATT AAACAGCTAG TGAGGGAGTG TAAGGAGGTT
    CTGAAGGGTG GCCTGTTAAT GAAGCAATAT TACCAATTTA TGTTGCGTGA GGTGTTAGAT
    GACTTGCAAA AGATTGATTG CAACATTGAT GCTTTTGAAG AGGACCTGCA TAAAATGTTG
    ATGGTTTACT TTGATTACAT GCGAAGCTGG ATCCAAATGC TACAGCAGTT ACCTCAAGCA
    TCTCATAGTT TAAAAAATCT CCTAGAGGAA GAATGGAATT TCACCAAAGA AATAACTCCA
    TATATAAGAG GAGGAGAAGC TCAAGCTGGA AAACTATTTT GTGATATTGC AGGAATGCTG
    CTGAAATCTA CAGGAATATT TTTAGATTCT GGACTACAAG ACAGTTGTGA TGAATTTTGG
    GCCAGTGCCG ATGACAGTAC TGCATCAGAT GAAATAAGGA GGTCTGTCAT AGAGACCAGC
    CGAGCACTCA AGGAACTGTT TCATGAAGCT AGAGAAAGGG CCTCCAAGGC TCTTGGATTT
    GCTAAAATGC TGAGGAAGGA CCTTGAAATA GCAGCAGAGT TTGCATTGTC AGCCCCTGTC
    CGAGATCTTC TGAATGCTCT GAAAACGAAA GAGTATGTGA AGGTACAAAT CCCTGGACTC
    GAGAATTTAC AAGTATTTGT CCCAAATAGT ATTGCAGGTG AAAAGTCTGT GATTTTGCAG
    CTCCTAAATG CGGCTGCTGG GAAAGATTGC TCAAAAGATT CAGATGAGGT TGCATATGAG
    GCCTATTTAA TAATGACCAA ACACAGTGAC AAAGACCATG AATTAGGTGA CATGTGGAGT
    GCATGGGAGG GCCAACCAGT CAAAGTGGTG CCACAAGTGG AAACTGTTGA TACGCTACGA
    AGCATGCAGG TTGACAACCT CCTTCTCGTA GTCATTCAGT CTGCCCACCT TGTGAGTCAG
    CGAAAAGCTT TCCAACAGTC TATAGAAGGG CTCATATCAC TCCACCAGGA ACAGACATCC
    AGCCAACCAG TCATTGCCAA AGCTCTGCAG CAACTAAAGA ATGATGCATT GCAGCTATGC
    AATAAGATAA GCAGTGCCAT TGACAGAGTT GATCATATGT TCACATCTGA GTTTGATGCT
    GAAGTTGATG AATCTGAATC TGCGACTTTG CAGCAATACT ACCGGGAAGC CATGATTCAG
    GCTTACAATT TTGGGTTTGA GTACCACAAA GAAGTTGTTC GTCTGATGTC TGGAGAATTC
    AGGCAGAAAA TTGGAGACAA ATATATCAGC TTTGCCAGAA AGTGGATGAA CTACGTGCTA
    ACAAAATGTG AGAGTGGCCG AGGCACTCGA CCCAGGTGGG CAACTCAAGG TTTTGATTTT
    CTTCAAGCCA TTGAACCAGC ATTTATTTCG GCTCTCCCAG AAGATGATTT CTTGAGTTTG
    CAAGCTCTGA TGAATGAGTG CATTGGCCAC GTTATTGGAA AACCTCACAG TCCTGTTACA
    GGTCTGTACC TTGCTATTCA TCGGAACAGT CCCCGTCCCG TGAAAGTACC TCGATGCCAT
    AGTGATCCAC CGAATCCTCA TCTTATCATC CCCACTCCTG AAGGCTTCAG CACACGGAGC
    GTCCCTTGCG ATGCACGGAA CCCTTGCAAC GTTACTGTCA GTCGCCCCGC TCCCATTGGT
    AGTGACTCAA CTCAACCTAA ATCTGTCAGC AGTGCAAATG ATACCAGGGG TTCCAGTGTC
    CCCGAAAATG ATCGACTGGC TTCAATAGCA GCCGAGTTGC AGTTCAGATC CCTTAGTCGT
    CACTCCAGTC CCACTGAGGA GCGAGAAGAA CCATCATATC CAAAAGGAGA TTCAAGTGGC
    TCATCCCGGC GGAGTTGGGA GCTCAGAACC TTAATCAACC AGACCAAAGA CACTGTTTCC
    AAACAAGCAC CAATAGAAGC TGTTCAGAAG TCTGTTCAGT TGTTTGAAGA AAGGAGATAC
    AGAGAGATGA GGAGGAAGAA TATCATTGGC CAAGTTTGCG ACACACCAAA ATCCTACGAT
    AATGTCATGC ATGTAGGGTT GAGAAAAGTG ACTTTCAAGT GGCAGAGAGG AAACAAAATT
    GGTGAAGGCC AATATGGGAA AGTATATACC TGCATTAGTG TTGACACTGG AGAGCTGATG
    GCTATGAAAG AAATAAGATT TCAACCTAAT GATCACAAAA CCATCAAAGA AACAGCTGAT
    GAACTGAAGA TTTTTGAAGG CATCAAACAC CCTAATCTTG TTCGGTATTT TGGTGTGGAG
    CTCCATAGGG AAGAGATGTA CATCTTCATG GAGTACTGTG ATGAGGGCAC GCTGGAGGAG
    GTGTCAAAAC TGGGCCTTCA GGAGCATGTC ATTAGGCTCT ACACAAAGCA GATCACCATT
    GCTATCAATG TACTACATGA ACACGGTATT GTTCATCGAG ACATTAAAGG AGCCAACATC
    TTTCTTACCT CATCCGGACT GATTAAACTG GGTGACTTTG GCTGCTCAGT AAAACTAAAG
    AACAACACTC AAACAATGCC TGGAGAAGTG AACAGTACAT TGGGTACTGC AGCGTACATG
    GCTCCAGAAG TAATTACTAG AGCAAAAGGA GAAGGGCATG GACGTGCAGC AGACATCTGG
    AGCCTGGGCT GTGTTGTTAT AGAGATGGTC ACTGGCAAGA GGCCTTGGCA CGAATATGAG
    CACAACTTCC AGATCATGTA TAGAGTGGGG ATGGGTCATA AACCTCCTAT TCCTGACAAA
    GTGAGTCCGG AGGGGAAAGA CTTCCTTTGC CACTGCCTGG AAAGTGACCC AAAGATGAGG
    TGGACAGCCA GCCAGCTCCT CGACCATCCT TTTGTCAAGG TTTGCACAGA TGAAGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33780
    Clone ID Related Accession (Same CDS sequence) XM_015284324.1 , XM_015284325.1 , XM_015284325.2 , XM_015284324.2
    Accession Version XM_015284324.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4539bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product mitogen-activated protein kinase kinase kinase 4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455837.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAAGCGTA TGTCAACAAA GCATCAGAAA AACAACGTCG GGAGACCTGC TAGCCGCTCT 
    ACTTTGAAAG AAAAGATGAG TATACCACCT CAATCCGTGC ATAAAGACAA TGGGAAAACC
    ATTGAGAACG TGGAGGAGCA CAGCTATAAG CAAGGGAAAA AAATGCGTGA CACCCTAAGG
    ACGACAGAAC GAGATCACAA GAAAAATGTG CAGTGCTCGT TTATGTTAGA CTCAGTTGGC
    GGGTCTCTGC CAAAAAAAAC AATTCCAGAT GTTGATCTCA ATAAGCCTTA CCTCAGCCTT
    GGCTGTAGCA ATGCTAAGCT TCCAGTCTCT GTGCCCATGC CAATCTCCAG AACTGCACGC
    CAGACTTCTA GGACTGATTG CCCAGCGGAC CGCTTAAAAT TCTTTGAAAC CCTGCGGCTT
    TTGTTAAAAC TTACCTCAGT CTCGAAAAAA AAGGACAGGG AGCAAAGAGG ACAGGAAAGC
    ACCTCTTCTG TCTGGCTGAA CCGATCCAAT GAACTGATCT GGCTAGAGCT GCAAGCTTGG
    CATGCTGGCC GGAGTATTAA TGACCAAGAC CTTTATCTCT ATGCAGCCCG TCAAGCTATC
    CCTGATATTA TCAATGAAAT CCTTACATTC AAAGTGAACT ATGGAAGCTT CTCCTATGTA
    AAAAATGGAG CCAGTCTCAA TGGTACGTCA GGAGAAGGAG TTTGCAAAGC AACACATGGA
    ACCAGTGCTT TGGGTTACTC AAGTTACCAC GAACACCTCC ATCGCCAGCG AATCTCATTT
    GAGCAAGTAA AGCGAATAAT GGAACTGCTA GAGTATATAG AAGCACTTTA TCCATCTCTG
    CAGGCTCTTC AAAAGGACTA TGAAAAGTAT GCTGCAAAAG ACTTCCAGGA CAGAGTGCAG
    GCACTCTGTC TGTGGTTGAA CATTACAAAA GACTTAAATC AAAAGCTAAG GATTATGGGG
    ACTGTTTTGG GCATCAAAAA TCTCTCTGAC ATTGGCTGGC CAGTGTTTGA AATTCCTTCT
    CCTCGACCGT CTAAGGACGA TGATCCAGAG GATGAAGGAG TTGATAGGGA AACTGAAGTA
    AAGGAATCCT CAGGAACATG CACAGAGGAG AGCGATGGCG AAGAACAAAT CTCTGAGGAG
    AATGTTGAGG AACTTAGACA AGCCATCAAG AACAATTTTA CTAATCAGCC AAATTTCGAC
    TTTCAGGACC ATTTTTCAAG GAAGCTTGAT AGGCTTGAAT CTGGGGATGA CTCTGCCTCT
    TGGATTATTC CAGAATGCAG TGCTGAGGCA GGCTGCAGCA AACACTGTTT GACTTCTATT
    TACAGGCCGT TTGTAGACAA AGCACTGAAG CAAATGGGGT TGAGGAAGCT AATTTTAAGA
    CTACACAAGC TAATGCATGG TTCATTGCAA AGGGCTCGTA TGGCGTTGGT AAGGAGTGAT
    TGCCCGCTGG AGTTTTCAGA ATGTCCAGAT CCCATGTTGT GTTCAGATTA CGTGCAGTTA
    TTAAATTCCC CACCTTGCTG TGAGCAAAAA TGCAGCACTG TATCATGGGA AGAGCTCAAA
    TCCATGGATT TGCCATCTTT TGAACCTGCA TTCTTGGTCC TTTGCCGAGT CCTGCTCAAT
    GTTATCCATG AATGTCTCAA GCTAAGGTTG GAACAAAGGC CTGCTGGGGA GCCATCTCTT
    TTGAGTATTA AACAGCTAGT GAGGGAGTGT AAGGAGGTTC TGAAGGGTGG CCTGTTAATG
    AAGCAATATT ACCAATTTAT GTTGCGTGAG GTGTTAGATG ACTTGCAAAA GATTGATTGC
    AACATTGATG CTTTTGAAGA GGACCTGCAT AAAATGTTGA TGGTTTACTT TGATTACATG
    CGAAGCTGGA TCCAAATGCT ACAGCAGTTA CCTCAAGCAT CTCATAGTTT AAAAAATCTC
    CTAGAGGAAG AATGGAATTT CACCAAAGAA ATAACTCCAT ATATAAGAGG AGGAGAAGCT
    CAAGCTGGAA AACTATTTTG TGATATTGCA GGAATGCTGC TGAAATCTAC AGGAATATTT
    TTAGATTCTG GACTACAAGA CAGTTGTGAT GAATTTTGGG CCAGTGCCGA TGACAGTACT
    GCATCAGATG AAATAAGGAG GTCTGTCATA GAGACCAGCC GAGCACTCAA GGAACTGTTT
    CATGAAGCTA GAGAAAGGGC CTCCAAGGCT CTTGGATTTG CTAAAATGCT GAGGAAGGAC
    CTTGAAATAG CAGCAGAGTT TGCATTGTCA GCCCCTGTCC GAGATCTTCT GAATGCTCTG
    AAAACGAAAG AGTATGTGAA GGTACAAATC CCTGGACTCG AGAATTTACA AGTATTTGTC
    CCAAATAGTA TTGCAGGTGA AAAGTCTGTG ATTTTGCAGC TCCTAAATGC GGCTGCTGGG
    AAAGATTGCT CAAAAGATTC AGATGAGGTT GCATATGAGG CCTATTTAAT AATGACCAAA
    CACAGTGACA AAGACCATGA ATTAGGTGAC ATGTGGAGTG CATGGGAGGG CCAACCAGTC
    AAAGTGGTGC CACAAGTGGA AACTGTTGAT ACGCTACGAA GCATGCAGGT TGACAACCTC
    CTTCTCGTAG TCATTCAGTC TGCCCACCTT GTGAGTCAGC GAAAAGCTTT CCAACAGTCT
    ATAGAAGGGC TCATATCACT CCACCAGGAA CAGACATCCA GCCAACCAGT CATTGCCAAA
    GCTCTGCAGC AACTAAAGAA TGATGCATTG CAGCTATGCA ATAAGATAAG CAGTGCCATT
    GACAGAGTTG ATCATATGTT CACATCTGAG TTTGATGCTG AAGTTGATGA ATCTGAATCT
    GCGACTTTGC AGCAATACTA CCGGGAAGCC ATGATTCAGG CTTACAATTT TGGGTTTGAG
    TACCACAAAG AAGTTGTTCG TCTGATGTCT GGAGAATTCA GGCAGAAAAT TGGAGACAAA
    TATATCAGCT TTGCCAGAAA GTGGATGAAC TACGTGCTAA CAAAATGTGA GAGTGGCCGA
    GGCACTCGAC CCAGGTGGGC AACTCAAGGT TTTGATTTTC TTCAAGCCAT TGAACCAGCA
    TTTATTTCGG CTCTCCCAGA AGATGATTTC TTGAGTTTGC AAGCTCTGAT GAATGAGTGC
    ATTGGCCACG TTATTGGAAA ACCTCACAGT CCTGTTACAG GTCTGTACCT TGCTATTCAT
    CGGAACAGTC CCCGTCCCGT GAAAGTACCT CGATGCCATA GTGATCCACC GAATCCTCAT
    CTTATCATCC CCACTCCTGA AGGCTTCAGC ACACGGAGCG TCCCTTGCGA TGCACGGAAC
    CCTTGCAACG TTACTGTCAG TCGCCCCGCT CCCATTGGTA GTGACTCAAC TCAACCTAAA
    TCTGTCAGCA GTGCAAATGA TACCAGGGGT TCCAGTGTCC CCGAAAATGA TCGACTGGCT
    TCAATAGCAG CCGAGTTGCA GTTCAGATCC CTTAGTCGTC ACTCCAGTCC CACTGAGGAG
    CGAGAAGAAC CATCATATCC AAAAGGAGAT TCAAGTGGCT CATCCCGGCG GAGTTGGGAG
    CTCAGAACCT TAATCAACCA GACCAAAGAC ACTGTTTCCA AACAAGCACC AATAGAAGCT
    GTTCAGAAGT CTGTTCAGTT GTTTGAAGAA AGGAGATACA GAGAGATGAG GAGGAAGAAT
    ATCATTGGCC AAGTTTGCGA CACACCAAAA TCCTACGATA ATGTCATGCA TGTAGGGTTG
    AGAAAAGTGA CTTTCAAGTG GCAGAGAGGA AACAAAATTG GTGAAGGCCA ATATGGGAAA
    GTATATACCT GCATTAGTGT TGACACTGGA GAGCTGATGG CTATGAAAGA AATAAGATTT
    CAACCTAATG ATCACAAAAC CATCAAAGAA ACAGCTGATG AACTGAAGAT TTTTGAAGGC
    ATCAAACACC CTAATCTTGT TCGGTATTTT GGTGTGGAGC TCCATAGGGA AGAGATGTAC
    ATCTTCATGG AGTACTGTGA TGAGGGCACG CTGGAGGAGG TGTCAAAACT GGGCCTTCAG
    GAGCATGTCA TTAGGCTCTA CACAAAGCAG ATCACCATTG CTATCAATGT ACTACATGAA
    CACGGTATTG TTCATCGAGA CATTAAAGGA GCCAACATCT TTCTTACCTC ATCCGGACTG
    ATTAAACTGG GTGACTTTGG CTGCTCAGTA AAACTAAAGA ACAACACTCA AACAATGCCT
    GGAGAAGTGA ACAGTACATT GGGTACTGCA GCGTACATGG CTCCAGAAGT AATTACTAGA
    GCAAAAGGAG AAGGGCATGG ACGTGCAGCA GACATCTGGA GCCTGGGCTG TGTTGTTATA
    GAGATGGTCA CTGGCAAGAG GCCTTGGCAC GAATATGAGC ACAACTTCCA GATCATGTAT
    AGAGTGGGGA TGGGTCATAA ACCTCCTATT CCTGACAAAG TGAGTCCGGA GGGGAAAGAC
    TTCCTTTGCC ACTGCCTGGA AAGTGACCCA AAGATGAGGT GGACAGCCAG CCAGCTCCTC
    GACCATCCTT TTGTCAAGGT TTGCACAGAT GAAGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139810.1
    CDS379..4917
    Translation

    Target ORF information:

    RefSeq Version XM_015284324.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284324.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAAGCGTA TGTCAACAAA GCATCAGAAA AACAACGTCG GGAGACCTGC TAGCCGCTCT 
    ACTTTGAAAG AAAAGATGAG TATACCACCT CAATCCGTGC ATAAAGACAA TGGGAAAACC
    ATTGAGAACG TGGAGGAGCA CAGCTATAAG CAAGGGAAAA AAATGCGTGA CACCCTAAGG
    ACGACAGAAC GAGATCACAA GAAAAATGTG CAGTGCTCGT TTATGTTAGA CTCAGTTGGC
    GGGTCTCTGC CAAAAAAAAC AATTCCAGAT GTTGATCTCA ATAAGCCTTA CCTCAGCCTT
    GGCTGTAGCA ATGCTAAGCT TCCAGTCTCT GTGCCCATGC CAATCTCCAG AACTGCACGC
    CAGACTTCTA GGACTGATTG CCCAGCGGAC CGCTTAAAAT TCTTTGAAAC CCTGCGGCTT
    TTGTTAAAAC TTACCTCAGT CTCGAAAAAA AAGGACAGGG AGCAAAGAGG ACAGGAAAGC
    ACCTCTTCTG TCTGGCTGAA CCGATCCAAT GAACTGATCT GGCTAGAGCT GCAAGCTTGG
    CATGCTGGCC GGAGTATTAA TGACCAAGAC CTTTATCTCT ATGCAGCCCG TCAAGCTATC
    CCTGATATTA TCAATGAAAT CCTTACATTC AAAGTGAACT ATGGAAGCTT CTCCTATGTA
    AAAAATGGAG CCAGTCTCAA TGGTACGTCA GGAGAAGGAG TTTGCAAAGC AACACATGGA
    ACCAGTGCTT TGGGTTACTC AAGTTACCAC GAACACCTCC ATCGCCAGCG AATCTCATTT
    GAGCAAGTAA AGCGAATAAT GGAACTGCTA GAGTATATAG AAGCACTTTA TCCATCTCTG
    CAGGCTCTTC AAAAGGACTA TGAAAAGTAT GCTGCAAAAG ACTTCCAGGA CAGAGTGCAG
    GCACTCTGTC TGTGGTTGAA CATTACAAAA GACTTAAATC AAAAGCTAAG GATTATGGGG
    ACTGTTTTGG GCATCAAAAA TCTCTCTGAC ATTGGCTGGC CAGTGTTTGA AATTCCTTCT
    CCTCGACCGT CTAAGGACGA TGATCCAGAG GATGAAGGAG TTGATAGGGA AACTGAAGTA
    AAGGAATCCT CAGGAACATG CACAGAGGAG AGCGATGGCG AAGAACAAAT CTCTGAGGAG
    AATGTTGAGG AACTTAGACA AGCCATCAAG AACAATTTTA CTAATCAGCC AAATTTCGAC
    TTTCAGGACC ATTTTTCAAG GAAGCTTGAT AGGCTTGAAT CTGGGGATGA CTCTGCCTCT
    TGGATTATTC CAGAATGCAG TGCTGAGGCA GGCTGCAGCA AACACTGTTT GACTTCTATT
    TACAGGCCGT TTGTAGACAA AGCACTGAAG CAAATGGGGT TGAGGAAGCT AATTTTAAGA
    CTACACAAGC TAATGCATGG TTCATTGCAA AGGGCTCGTA TGGCGTTGGT AAGGAGTGAT
    TGCCCGCTGG AGTTTTCAGA ATGTCCAGAT CCCATGTTGT GTTCAGATTA CGTGCAGTTA
    TTAAATTCCC CACCTTGCTG TGAGCAAAAA TGCAGCACTG TATCATGGGA AGAGCTCAAA
    TCCATGGATT TGCCATCTTT TGAACCTGCA TTCTTGGTCC TTTGCCGAGT CCTGCTCAAT
    GTTATCCATG AATGTCTCAA GCTAAGGTTG GAACAAAGGC CTGCTGGGGA GCCATCTCTT
    TTGAGTATTA AACAGCTAGT GAGGGAGTGT AAGGAGGTTC TGAAGGGTGG CCTGTTAATG
    AAGCAATATT ACCAATTTAT GTTGCGTGAG GTGTTAGATG ACTTGCAAAA GATTGATTGC
    AACATTGATG CTTTTGAAGA GGACCTGCAT AAAATGTTGA TGGTTTACTT TGATTACATG
    CGAAGCTGGA TCCAAATGCT ACAGCAGTTA CCTCAAGCAT CTCATAGTTT AAAAAATCTC
    CTAGAGGAAG AATGGAATTT CACCAAAGAA ATAACTCCAT ATATAAGAGG AGGAGAAGCT
    CAAGCTGGAA AACTATTTTG TGATATTGCA GGAATGCTGC TGAAATCTAC AGGAATATTT
    TTAGATTCTG GACTACAAGA CAGTTGTGAT GAATTTTGGG CCAGTGCCGA TGACAGTACT
    GCATCAGATG AAATAAGGAG GTCTGTCATA GAGACCAGCC GAGCACTCAA GGAACTGTTT
    CATGAAGCTA GAGAAAGGGC CTCCAAGGCT CTTGGATTTG CTAAAATGCT GAGGAAGGAC
    CTTGAAATAG CAGCAGAGTT TGCATTGTCA GCCCCTGTCC GAGATCTTCT GAATGCTCTG
    AAAACGAAAG AGTATGTGAA GGTACAAATC CCTGGACTCG AGAATTTACA AGTATTTGTC
    CCAAATAGTA TTGCAGGTGA AAAGTCTGTG ATTTTGCAGC TCCTAAATGC GGCTGCTGGG
    AAAGATTGCT CAAAAGATTC AGATGAGGTT GCATATGAGG CCTATTTAAT AATGACCAAA
    CACAGTGACA AAGACCATGA ATTAGGTGAC ATGTGGAGTG CATGGGAGGG CCAACCAGTC
    AAAGTGGTGC CACAAGTGGA AACTGTTGAT ACGCTACGAA GCATGCAGGT TGACAACCTC
    CTTCTCGTAG TCATTCAGTC TGCCCACCTT GTGAGTCAGC GAAAAGCTTT CCAACAGTCT
    ATAGAAGGGC TCATATCACT CCACCAGGAA CAGACATCCA GCCAACCAGT CATTGCCAAA
    GCTCTGCAGC AACTAAAGAA TGATGCATTG CAGCTATGCA ATAAGATAAG CAGTGCCATT
    GACAGAGTTG ATCATATGTT CACATCTGAG TTTGATGCTG AAGTTGATGA ATCTGAATCT
    GCGACTTTGC AGCAATACTA CCGGGAAGCC ATGATTCAGG CTTACAATTT TGGGTTTGAG
    TACCACAAAG AAGTTGTTCG TCTGATGTCT GGAGAATTCA GGCAGAAAAT TGGAGACAAA
    TATATCAGCT TTGCCAGAAA GTGGATGAAC TACGTGCTAA CAAAATGTGA GAGTGGCCGA
    GGCACTCGAC CCAGGTGGGC AACTCAAGGT TTTGATTTTC TTCAAGCCAT TGAACCAGCA
    TTTATTTCGG CTCTCCCAGA AGATGATTTC TTGAGTTTGC AAGCTCTGAT GAATGAGTGC
    ATTGGCCACG TTATTGGAAA ACCTCACAGT CCTGTTACAG GTCTGTACCT TGCTATTCAT
    CGGAACAGTC CCCGTCCCGT GAAAGTACCT CGATGCCATA GTGATCCACC GAATCCTCAT
    CTTATCATCC CCACTCCTGA AGGCTTCAGC ACACGGAGCG TCCCTTGCGA TGCACGGAAC
    CCTTGCAACG TTACTGTCAG TCGCCCCGCT CCCATTGGTA GTGACTCAAC TCAACCTAAA
    TCTGTCAGCA GTGCAAATGA TACCAGGGGT TCCAGTGTCC CCGAAAATGA TCGACTGGCT
    TCAATAGCAG CCGAGTTGCA GTTCAGATCC CTTAGTCGTC ACTCCAGTCC CACTGAGGAG
    CGAGAAGAAC CATCATATCC AAAAGGAGAT TCAAGTGGCT CATCCCGGCG GAGTTGGGAG
    CTCAGAACCT TAATCAACCA GACCAAAGAC ACTGTTTCCA AACAAGCACC AATAGAAGCT
    GTTCAGAAGT CTGTTCAGTT GTTTGAAGAA AGGAGATACA GAGAGATGAG GAGGAAGAAT
    ATCATTGGCC AAGTTTGCGA CACACCAAAA TCCTACGATA ATGTCATGCA TGTAGGGTTG
    AGAAAAGTGA CTTTCAAGTG GCAGAGAGGA AACAAAATTG GTGAAGGCCA ATATGGGAAA
    GTATATACCT GCATTAGTGT TGACACTGGA GAGCTGATGG CTATGAAAGA AATAAGATTT
    CAACCTAATG ATCACAAAAC CATCAAAGAA ACAGCTGATG AACTGAAGAT TTTTGAAGGC
    ATCAAACACC CTAATCTTGT TCGGTATTTT GGTGTGGAGC TCCATAGGGA AGAGATGTAC
    ATCTTCATGG AGTACTGTGA TGAGGGCACG CTGGAGGAGG TGTCAAAACT GGGCCTTCAG
    GAGCATGTCA TTAGGCTCTA CACAAAGCAG ATCACCATTG CTATCAATGT ACTACATGAA
    CACGGTATTG TTCATCGAGA CATTAAAGGA GCCAACATCT TTCTTACCTC ATCCGGACTG
    ATTAAACTGG GTGACTTTGG CTGCTCAGTA AAACTAAAGA ACAACACTCA AACAATGCCT
    GGAGAAGTGA ACAGTACATT GGGTACTGCA GCGTACATGG CTCCAGAAGT AATTACTAGA
    GCAAAAGGAG AAGGGCATGG ACGTGCAGCA GACATCTGGA GCCTGGGCTG TGTTGTTATA
    GAGATGGTCA CTGGCAAGAG GCCTTGGCAC GAATATGAGC ACAACTTCCA GATCATGTAT
    AGAGTGGGGA TGGGTCATAA ACCTCCTATT CCTGACAAAG TGAGTCCGGA GGGGAAAGAC
    TTCCTTTGCC ACTGCCTGGA AAGTGACCCA AAGATGAGGT GGACAGCCAG CCAGCTCCTC
    GACCATCCTT TTGTCAAGGT TTGCACAGAT GAAGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33780
    Clone ID Related Accession (Same CDS sequence) XM_015284324.1 , XM_015284325.1 , XM_015284325.2 , XM_015284324.2
    Accession Version XM_015284325.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4539bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product mitogen-activated protein kinase kinase kinase 4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455837.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAAGCGTA TGTCAACAAA GCATCAGAAA AACAACGTCG GGAGACCTGC TAGCCGCTCT 
    ACTTTGAAAG AAAAGATGAG TATACCACCT CAATCCGTGC ATAAAGACAA TGGGAAAACC
    ATTGAGAACG TGGAGGAGCA CAGCTATAAG CAAGGGAAAA AAATGCGTGA CACCCTAAGG
    ACGACAGAAC GAGATCACAA GAAAAATGTG CAGTGCTCGT TTATGTTAGA CTCAGTTGGC
    GGGTCTCTGC CAAAAAAAAC AATTCCAGAT GTTGATCTCA ATAAGCCTTA CCTCAGCCTT
    GGCTGTAGCA ATGCTAAGCT TCCAGTCTCT GTGCCCATGC CAATCTCCAG AACTGCACGC
    CAGACTTCTA GGACTGATTG CCCAGCGGAC CGCTTAAAAT TCTTTGAAAC CCTGCGGCTT
    TTGTTAAAAC TTACCTCAGT CTCGAAAAAA AAGGACAGGG AGCAAAGAGG ACAGGAAAGC
    ACCTCTTCTG TCTGGCTGAA CCGATCCAAT GAACTGATCT GGCTAGAGCT GCAAGCTTGG
    CATGCTGGCC GGAGTATTAA TGACCAAGAC CTTTATCTCT ATGCAGCCCG TCAAGCTATC
    CCTGATATTA TCAATGAAAT CCTTACATTC AAAGTGAACT ATGGAAGCTT CTCCTATGTA
    AAAAATGGAG CCAGTCTCAA TGGTACGTCA GGAGAAGGAG TTTGCAAAGC AACACATGGA
    ACCAGTGCTT TGGGTTACTC AAGTTACCAC GAACACCTCC ATCGCCAGCG AATCTCATTT
    GAGCAAGTAA AGCGAATAAT GGAACTGCTA GAGTATATAG AAGCACTTTA TCCATCTCTG
    CAGGCTCTTC AAAAGGACTA TGAAAAGTAT GCTGCAAAAG ACTTCCAGGA CAGAGTGCAG
    GCACTCTGTC TGTGGTTGAA CATTACAAAA GACTTAAATC AAAAGCTAAG GATTATGGGG
    ACTGTTTTGG GCATCAAAAA TCTCTCTGAC ATTGGCTGGC CAGTGTTTGA AATTCCTTCT
    CCTCGACCGT CTAAGGACGA TGATCCAGAG GATGAAGGAG TTGATAGGGA AACTGAAGTA
    AAGGAATCCT CAGGAACATG CACAGAGGAG AGCGATGGCG AAGAACAAAT CTCTGAGGAG
    AATGTTGAGG AACTTAGACA AGCCATCAAG AACAATTTTA CTAATCAGCC AAATTTCGAC
    TTTCAGGACC ATTTTTCAAG GAAGCTTGAT AGGCTTGAAT CTGGGGATGA CTCTGCCTCT
    TGGATTATTC CAGAATGCAG TGCTGAGGCA GGCTGCAGCA AACACTGTTT GACTTCTATT
    TACAGGCCGT TTGTAGACAA AGCACTGAAG CAAATGGGGT TGAGGAAGCT AATTTTAAGA
    CTACACAAGC TAATGCATGG TTCATTGCAA AGGGCTCGTA TGGCGTTGGT AAGGAGTGAT
    TGCCCGCTGG AGTTTTCAGA ATGTCCAGAT CCCATGTTGT GTTCAGATTA CGTGCAGTTA
    TTAAATTCCC CACCTTGCTG TGAGCAAAAA TGCAGCACTG TATCATGGGA AGAGCTCAAA
    TCCATGGATT TGCCATCTTT TGAACCTGCA TTCTTGGTCC TTTGCCGAGT CCTGCTCAAT
    GTTATCCATG AATGTCTCAA GCTAAGGTTG GAACAAAGGC CTGCTGGGGA GCCATCTCTT
    TTGAGTATTA AACAGCTAGT GAGGGAGTGT AAGGAGGTTC TGAAGGGTGG CCTGTTAATG
    AAGCAATATT ACCAATTTAT GTTGCGTGAG GTGTTAGATG ACTTGCAAAA GATTGATTGC
    AACATTGATG CTTTTGAAGA GGACCTGCAT AAAATGTTGA TGGTTTACTT TGATTACATG
    CGAAGCTGGA TCCAAATGCT ACAGCAGTTA CCTCAAGCAT CTCATAGTTT AAAAAATCTC
    CTAGAGGAAG AATGGAATTT CACCAAAGAA ATAACTCCAT ATATAAGAGG AGGAGAAGCT
    CAAGCTGGAA AACTATTTTG TGATATTGCA GGAATGCTGC TGAAATCTAC AGGAATATTT
    TTAGATTCTG GACTACAAGA CAGTTGTGAT GAATTTTGGG CCAGTGCCGA TGACAGTACT
    GCATCAGATG AAATAAGGAG GTCTGTCATA GAGACCAGCC GAGCACTCAA GGAACTGTTT
    CATGAAGCTA GAGAAAGGGC CTCCAAGGCT CTTGGATTTG CTAAAATGCT GAGGAAGGAC
    CTTGAAATAG CAGCAGAGTT TGCATTGTCA GCCCCTGTCC GAGATCTTCT GAATGCTCTG
    AAAACGAAAG AGTATGTGAA GGTACAAATC CCTGGACTCG AGAATTTACA AGTATTTGTC
    CCAAATAGTA TTGCAGGTGA AAAGTCTGTG ATTTTGCAGC TCCTAAATGC GGCTGCTGGG
    AAAGATTGCT CAAAAGATTC AGATGAGGTT GCATATGAGG CCTATTTAAT AATGACCAAA
    CACAGTGACA AAGACCATGA ATTAGGTGAC ATGTGGAGTG CATGGGAGGG CCAACCAGTC
    AAAGTGGTGC CACAAGTGGA AACTGTTGAT ACGCTACGAA GCATGCAGGT TGACAACCTC
    CTTCTCGTAG TCATTCAGTC TGCCCACCTT GTGAGTCAGC GAAAAGCTTT CCAACAGTCT
    ATAGAAGGGC TCATATCACT CCACCAGGAA CAGACATCCA GCCAACCAGT CATTGCCAAA
    GCTCTGCAGC AACTAAAGAA TGATGCATTG CAGCTATGCA ATAAGATAAG CAGTGCCATT
    GACAGAGTTG ATCATATGTT CACATCTGAG TTTGATGCTG AAGTTGATGA ATCTGAATCT
    GCGACTTTGC AGCAATACTA CCGGGAAGCC ATGATTCAGG CTTACAATTT TGGGTTTGAG
    TACCACAAAG AAGTTGTTCG TCTGATGTCT GGAGAATTCA GGCAGAAAAT TGGAGACAAA
    TATATCAGCT TTGCCAGAAA GTGGATGAAC TACGTGCTAA CAAAATGTGA GAGTGGCCGA
    GGCACTCGAC CCAGGTGGGC AACTCAAGGT TTTGATTTTC TTCAAGCCAT TGAACCAGCA
    TTTATTTCGG CTCTCCCAGA AGATGATTTC TTGAGTTTGC AAGCTCTGAT GAATGAGTGC
    ATTGGCCACG TTATTGGAAA ACCTCACAGT CCTGTTACAG GTCTGTACCT TGCTATTCAT
    CGGAACAGTC CCCGTCCCGT GAAAGTACCT CGATGCCATA GTGATCCACC GAATCCTCAT
    CTTATCATCC CCACTCCTGA AGGCTTCAGC ACACGGAGCG TCCCTTGCGA TGCACGGAAC
    CCTTGCAACG TTACTGTCAG TCGCCCCGCT CCCATTGGTA GTGACTCAAC TCAACCTAAA
    TCTGTCAGCA GTGCAAATGA TACCAGGGGT TCCAGTGTCC CCGAAAATGA TCGACTGGCT
    TCAATAGCAG CCGAGTTGCA GTTCAGATCC CTTAGTCGTC ACTCCAGTCC CACTGAGGAG
    CGAGAAGAAC CATCATATCC AAAAGGAGAT TCAAGTGGCT CATCCCGGCG GAGTTGGGAG
    CTCAGAACCT TAATCAACCA GACCAAAGAC ACTGTTTCCA AACAAGCACC AATAGAAGCT
    GTTCAGAAGT CTGTTCAGTT GTTTGAAGAA AGGAGATACA GAGAGATGAG GAGGAAGAAT
    ATCATTGGCC AAGTTTGCGA CACACCAAAA TCCTACGATA ATGTCATGCA TGTAGGGTTG
    AGAAAAGTGA CTTTCAAGTG GCAGAGAGGA AACAAAATTG GTGAAGGCCA ATATGGGAAA
    GTATATACCT GCATTAGTGT TGACACTGGA GAGCTGATGG CTATGAAAGA AATAAGATTT
    CAACCTAATG ATCACAAAAC CATCAAAGAA ACAGCTGATG AACTGAAGAT TTTTGAAGGC
    ATCAAACACC CTAATCTTGT TCGGTATTTT GGTGTGGAGC TCCATAGGGA AGAGATGTAC
    ATCTTCATGG AGTACTGTGA TGAGGGCACG CTGGAGGAGG TGTCAAAACT GGGCCTTCAG
    GAGCATGTCA TTAGGCTCTA CACAAAGCAG ATCACCATTG CTATCAATGT ACTACATGAA
    CACGGTATTG TTCATCGAGA CATTAAAGGA GCCAACATCT TTCTTACCTC ATCCGGACTG
    ATTAAACTGG GTGACTTTGG CTGCTCAGTA AAACTAAAGA ACAACACTCA AACAATGCCT
    GGAGAAGTGA ACAGTACATT GGGTACTGCA GCGTACATGG CTCCAGAAGT AATTACTAGA
    GCAAAAGGAG AAGGGCATGG ACGTGCAGCA GACATCTGGA GCCTGGGCTG TGTTGTTATA
    GAGATGGTCA CTGGCAAGAG GCCTTGGCAC GAATATGAGC ACAACTTCCA GATCATGTAT
    AGAGTGGGGA TGGGTCATAA ACCTCCTATT CCTGACAAAG TGAGTCCGGA GGGGAAAGAC
    TTCCTTTGCC ACTGCCTGGA AAGTGACCCA AAGATGAGGT GGACAGCCAG CCAGCTCCTC
    GACCATCCTT TTGTCAAGGT TTGCACAGAT GAAGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139811.1
    CDS217..4755
    Translation

    Target ORF information:

    RefSeq Version XM_015284325.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284325.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAAGCGTA TGTCAACAAA GCATCAGAAA AACAACGTCG GGAGACCTGC TAGCCGCTCT 
    ACTTTGAAAG AAAAGATGAG TATACCACCT CAATCCGTGC ATAAAGACAA TGGGAAAACC
    ATTGAGAACG TGGAGGAGCA CAGCTATAAG CAAGGGAAAA AAATGCGTGA CACCCTAAGG
    ACGACAGAAC GAGATCACAA GAAAAATGTG CAGTGCTCGT TTATGTTAGA CTCAGTTGGC
    GGGTCTCTGC CAAAAAAAAC AATTCCAGAT GTTGATCTCA ATAAGCCTTA CCTCAGCCTT
    GGCTGTAGCA ATGCTAAGCT TCCAGTCTCT GTGCCCATGC CAATCTCCAG AACTGCACGC
    CAGACTTCTA GGACTGATTG CCCAGCGGAC CGCTTAAAAT TCTTTGAAAC CCTGCGGCTT
    TTGTTAAAAC TTACCTCAGT CTCGAAAAAA AAGGACAGGG AGCAAAGAGG ACAGGAAAGC
    ACCTCTTCTG TCTGGCTGAA CCGATCCAAT GAACTGATCT GGCTAGAGCT GCAAGCTTGG
    CATGCTGGCC GGAGTATTAA TGACCAAGAC CTTTATCTCT ATGCAGCCCG TCAAGCTATC
    CCTGATATTA TCAATGAAAT CCTTACATTC AAAGTGAACT ATGGAAGCTT CTCCTATGTA
    AAAAATGGAG CCAGTCTCAA TGGTACGTCA GGAGAAGGAG TTTGCAAAGC AACACATGGA
    ACCAGTGCTT TGGGTTACTC AAGTTACCAC GAACACCTCC ATCGCCAGCG AATCTCATTT
    GAGCAAGTAA AGCGAATAAT GGAACTGCTA GAGTATATAG AAGCACTTTA TCCATCTCTG
    CAGGCTCTTC AAAAGGACTA TGAAAAGTAT GCTGCAAAAG ACTTCCAGGA CAGAGTGCAG
    GCACTCTGTC TGTGGTTGAA CATTACAAAA GACTTAAATC AAAAGCTAAG GATTATGGGG
    ACTGTTTTGG GCATCAAAAA TCTCTCTGAC ATTGGCTGGC CAGTGTTTGA AATTCCTTCT
    CCTCGACCGT CTAAGGACGA TGATCCAGAG GATGAAGGAG TTGATAGGGA AACTGAAGTA
    AAGGAATCCT CAGGAACATG CACAGAGGAG AGCGATGGCG AAGAACAAAT CTCTGAGGAG
    AATGTTGAGG AACTTAGACA AGCCATCAAG AACAATTTTA CTAATCAGCC AAATTTCGAC
    TTTCAGGACC ATTTTTCAAG GAAGCTTGAT AGGCTTGAAT CTGGGGATGA CTCTGCCTCT
    TGGATTATTC CAGAATGCAG TGCTGAGGCA GGCTGCAGCA AACACTGTTT GACTTCTATT
    TACAGGCCGT TTGTAGACAA AGCACTGAAG CAAATGGGGT TGAGGAAGCT AATTTTAAGA
    CTACACAAGC TAATGCATGG TTCATTGCAA AGGGCTCGTA TGGCGTTGGT AAGGAGTGAT
    TGCCCGCTGG AGTTTTCAGA ATGTCCAGAT CCCATGTTGT GTTCAGATTA CGTGCAGTTA
    TTAAATTCCC CACCTTGCTG TGAGCAAAAA TGCAGCACTG TATCATGGGA AGAGCTCAAA
    TCCATGGATT TGCCATCTTT TGAACCTGCA TTCTTGGTCC TTTGCCGAGT CCTGCTCAAT
    GTTATCCATG AATGTCTCAA GCTAAGGTTG GAACAAAGGC CTGCTGGGGA GCCATCTCTT
    TTGAGTATTA AACAGCTAGT GAGGGAGTGT AAGGAGGTTC TGAAGGGTGG CCTGTTAATG
    AAGCAATATT ACCAATTTAT GTTGCGTGAG GTGTTAGATG ACTTGCAAAA GATTGATTGC
    AACATTGATG CTTTTGAAGA GGACCTGCAT AAAATGTTGA TGGTTTACTT TGATTACATG
    CGAAGCTGGA TCCAAATGCT ACAGCAGTTA CCTCAAGCAT CTCATAGTTT AAAAAATCTC
    CTAGAGGAAG AATGGAATTT CACCAAAGAA ATAACTCCAT ATATAAGAGG AGGAGAAGCT
    CAAGCTGGAA AACTATTTTG TGATATTGCA GGAATGCTGC TGAAATCTAC AGGAATATTT
    TTAGATTCTG GACTACAAGA CAGTTGTGAT GAATTTTGGG CCAGTGCCGA TGACAGTACT
    GCATCAGATG AAATAAGGAG GTCTGTCATA GAGACCAGCC GAGCACTCAA GGAACTGTTT
    CATGAAGCTA GAGAAAGGGC CTCCAAGGCT CTTGGATTTG CTAAAATGCT GAGGAAGGAC
    CTTGAAATAG CAGCAGAGTT TGCATTGTCA GCCCCTGTCC GAGATCTTCT GAATGCTCTG
    AAAACGAAAG AGTATGTGAA GGTACAAATC CCTGGACTCG AGAATTTACA AGTATTTGTC
    CCAAATAGTA TTGCAGGTGA AAAGTCTGTG ATTTTGCAGC TCCTAAATGC GGCTGCTGGG
    AAAGATTGCT CAAAAGATTC AGATGAGGTT GCATATGAGG CCTATTTAAT AATGACCAAA
    CACAGTGACA AAGACCATGA ATTAGGTGAC ATGTGGAGTG CATGGGAGGG CCAACCAGTC
    AAAGTGGTGC CACAAGTGGA AACTGTTGAT ACGCTACGAA GCATGCAGGT TGACAACCTC
    CTTCTCGTAG TCATTCAGTC TGCCCACCTT GTGAGTCAGC GAAAAGCTTT CCAACAGTCT
    ATAGAAGGGC TCATATCACT CCACCAGGAA CAGACATCCA GCCAACCAGT CATTGCCAAA
    GCTCTGCAGC AACTAAAGAA TGATGCATTG CAGCTATGCA ATAAGATAAG CAGTGCCATT
    GACAGAGTTG ATCATATGTT CACATCTGAG TTTGATGCTG AAGTTGATGA ATCTGAATCT
    GCGACTTTGC AGCAATACTA CCGGGAAGCC ATGATTCAGG CTTACAATTT TGGGTTTGAG
    TACCACAAAG AAGTTGTTCG TCTGATGTCT GGAGAATTCA GGCAGAAAAT TGGAGACAAA
    TATATCAGCT TTGCCAGAAA GTGGATGAAC TACGTGCTAA CAAAATGTGA GAGTGGCCGA
    GGCACTCGAC CCAGGTGGGC AACTCAAGGT TTTGATTTTC TTCAAGCCAT TGAACCAGCA
    TTTATTTCGG CTCTCCCAGA AGATGATTTC TTGAGTTTGC AAGCTCTGAT GAATGAGTGC
    ATTGGCCACG TTATTGGAAA ACCTCACAGT CCTGTTACAG GTCTGTACCT TGCTATTCAT
    CGGAACAGTC CCCGTCCCGT GAAAGTACCT CGATGCCATA GTGATCCACC GAATCCTCAT
    CTTATCATCC CCACTCCTGA AGGCTTCAGC ACACGGAGCG TCCCTTGCGA TGCACGGAAC
    CCTTGCAACG TTACTGTCAG TCGCCCCGCT CCCATTGGTA GTGACTCAAC TCAACCTAAA
    TCTGTCAGCA GTGCAAATGA TACCAGGGGT TCCAGTGTCC CCGAAAATGA TCGACTGGCT
    TCAATAGCAG CCGAGTTGCA GTTCAGATCC CTTAGTCGTC ACTCCAGTCC CACTGAGGAG
    CGAGAAGAAC CATCATATCC AAAAGGAGAT TCAAGTGGCT CATCCCGGCG GAGTTGGGAG
    CTCAGAACCT TAATCAACCA GACCAAAGAC ACTGTTTCCA AACAAGCACC AATAGAAGCT
    GTTCAGAAGT CTGTTCAGTT GTTTGAAGAA AGGAGATACA GAGAGATGAG GAGGAAGAAT
    ATCATTGGCC AAGTTTGCGA CACACCAAAA TCCTACGATA ATGTCATGCA TGTAGGGTTG
    AGAAAAGTGA CTTTCAAGTG GCAGAGAGGA AACAAAATTG GTGAAGGCCA ATATGGGAAA
    GTATATACCT GCATTAGTGT TGACACTGGA GAGCTGATGG CTATGAAAGA AATAAGATTT
    CAACCTAATG ATCACAAAAC CATCAAAGAA ACAGCTGATG AACTGAAGAT TTTTGAAGGC
    ATCAAACACC CTAATCTTGT TCGGTATTTT GGTGTGGAGC TCCATAGGGA AGAGATGTAC
    ATCTTCATGG AGTACTGTGA TGAGGGCACG CTGGAGGAGG TGTCAAAACT GGGCCTTCAG
    GAGCATGTCA TTAGGCTCTA CACAAAGCAG ATCACCATTG CTATCAATGT ACTACATGAA
    CACGGTATTG TTCATCGAGA CATTAAAGGA GCCAACATCT TTCTTACCTC ATCCGGACTG
    ATTAAACTGG GTGACTTTGG CTGCTCAGTA AAACTAAAGA ACAACACTCA AACAATGCCT
    GGAGAAGTGA ACAGTACATT GGGTACTGCA GCGTACATGG CTCCAGAAGT AATTACTAGA
    GCAAAAGGAG AAGGGCATGG ACGTGCAGCA GACATCTGGA GCCTGGGCTG TGTTGTTATA
    GAGATGGTCA CTGGCAAGAG GCCTTGGCAC GAATATGAGC ACAACTTCCA GATCATGTAT
    AGAGTGGGGA TGGGTCATAA ACCTCCTATT CCTGACAAAG TGAGTCCGGA GGGGAAAGAC
    TTCCTTTGCC ACTGCCTGGA AAGTGACCCA AAGATGAGGT GGACAGCCAG CCAGCTCCTC
    GACCATCCTT TTGTCAAGGT TTGCACAGAT GAAGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33780
    Clone ID Related Accession (Same CDS sequence) XM_015284324.1 , XM_015284325.1 , XM_015284325.2 , XM_015284324.2
    Accession Version XM_015284325.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4539bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product mitogen-activated protein kinase kinase kinase 4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015284325.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAAGCGTA TGTCAACAAA GCATCAGAAA AACAACGTCG GGAGACCTGC TAGCCGCTCT 
    ACTTTGAAAG AAAAGATGAG TATACCACCT CAATCCGTGC ATAAAGACAA TGGGAAAACC
    ATTGAGAACG TGGAGGAGCA CAGCTATAAG CAAGGGAAAA AAATGCGTGA CACCCTAAGG
    ACGACAGAAC GAGATCACAA GAAAAATGTG CAGTGCTCGT TTATGTTAGA CTCAGTTGGC
    GGGTCTCTGC CAAAAAAAAC AATTCCAGAT GTTGATCTCA ATAAGCCTTA CCTCAGCCTT
    GGCTGTAGCA ATGCTAAGCT TCCAGTCTCT GTGCCCATGC CAATCTCCAG AACTGCACGC
    CAGACTTCTA GGACTGATTG CCCAGCGGAC CGCTTAAAAT TCTTTGAAAC CCTGCGGCTT
    TTGTTAAAAC TTACCTCAGT CTCGAAAAAA AAGGACAGGG AGCAAAGAGG ACAGGAAAGC
    ACCTCTTCTG TCTGGCTGAA CCGATCCAAT GAACTGATCT GGCTAGAGCT GCAAGCTTGG
    CATGCTGGCC GGAGTATTAA TGACCAAGAC CTTTATCTCT ATGCAGCCCG TCAAGCTATC
    CCTGATATTA TCAATGAAAT CCTTACATTC AAAGTGAACT ATGGAAGCTT CTCCTATGTA
    AAAAATGGAG CCAGTCTCAA TGGTACGTCA GGAGAAGGAG TTTGCAAAGC AACACATGGA
    ACCAGTGCTT TGGGTTACTC AAGTTACCAC GAACACCTCC ATCGCCAGCG AATCTCATTT
    GAGCAAGTAA AGCGAATAAT GGAACTGCTA GAGTATATAG AAGCACTTTA TCCATCTCTG
    CAGGCTCTTC AAAAGGACTA TGAAAAGTAT GCTGCAAAAG ACTTCCAGGA CAGAGTGCAG
    GCACTCTGTC TGTGGTTGAA CATTACAAAA GACTTAAATC AAAAGCTAAG GATTATGGGG
    ACTGTTTTGG GCATCAAAAA TCTCTCTGAC ATTGGCTGGC CAGTGTTTGA AATTCCTTCT
    CCTCGACCGT CTAAGGACGA TGATCCAGAG GATGAAGGAG TTGATAGGGA AACTGAAGTA
    AAGGAATCCT CAGGAACATG CACAGAGGAG AGCGATGGCG AAGAACAAAT CTCTGAGGAG
    AATGTTGAGG AACTTAGACA AGCCATCAAG AACAATTTTA CTAATCAGCC AAATTTCGAC
    TTTCAGGACC ATTTTTCAAG GAAGCTTGAT AGGCTTGAAT CTGGGGATGA CTCTGCCTCT
    TGGATTATTC CAGAATGCAG TGCTGAGGCA GGCTGCAGCA AACACTGTTT GACTTCTATT
    TACAGGCCGT TTGTAGACAA AGCACTGAAG CAAATGGGGT TGAGGAAGCT AATTTTAAGA
    CTACACAAGC TAATGCATGG TTCATTGCAA AGGGCTCGTA TGGCGTTGGT AAGGAGTGAT
    TGCCCGCTGG AGTTTTCAGA ATGTCCAGAT CCCATGTTGT GTTCAGATTA CGTGCAGTTA
    TTAAATTCCC CACCTTGCTG TGAGCAAAAA TGCAGCACTG TATCATGGGA AGAGCTCAAA
    TCCATGGATT TGCCATCTTT TGAACCTGCA TTCTTGGTCC TTTGCCGAGT CCTGCTCAAT
    GTTATCCATG AATGTCTCAA GCTAAGGTTG GAACAAAGGC CTGCTGGGGA GCCATCTCTT
    TTGAGTATTA AACAGCTAGT GAGGGAGTGT AAGGAGGTTC TGAAGGGTGG CCTGTTAATG
    AAGCAATATT ACCAATTTAT GTTGCGTGAG GTGTTAGATG ACTTGCAAAA GATTGATTGC
    AACATTGATG CTTTTGAAGA GGACCTGCAT AAAATGTTGA TGGTTTACTT TGATTACATG
    CGAAGCTGGA TCCAAATGCT ACAGCAGTTA CCTCAAGCAT CTCATAGTTT AAAAAATCTC
    CTAGAGGAAG AATGGAATTT CACCAAAGAA ATAACTCCAT ATATAAGAGG AGGAGAAGCT
    CAAGCTGGAA AACTATTTTG TGATATTGCA GGAATGCTGC TGAAATCTAC AGGAATATTT
    TTAGATTCTG GACTACAAGA CAGTTGTGAT GAATTTTGGG CCAGTGCCGA TGACAGTACT
    GCATCAGATG AAATAAGGAG GTCTGTCATA GAGACCAGCC GAGCACTCAA GGAACTGTTT
    CATGAAGCTA GAGAAAGGGC CTCCAAGGCT CTTGGATTTG CTAAAATGCT GAGGAAGGAC
    CTTGAAATAG CAGCAGAGTT TGCATTGTCA GCCCCTGTCC GAGATCTTCT GAATGCTCTG
    AAAACGAAAG AGTATGTGAA GGTACAAATC CCTGGACTCG AGAATTTACA AGTATTTGTC
    CCAAATAGTA TTGCAGGTGA AAAGTCTGTG ATTTTGCAGC TCCTAAATGC GGCTGCTGGG
    AAAGATTGCT CAAAAGATTC AGATGAGGTT GCATATGAGG CCTATTTAAT AATGACCAAA
    CACAGTGACA AAGACCATGA ATTAGGTGAC ATGTGGAGTG CATGGGAGGG CCAACCAGTC
    AAAGTGGTGC CACAAGTGGA AACTGTTGAT ACGCTACGAA GCATGCAGGT TGACAACCTC
    CTTCTCGTAG TCATTCAGTC TGCCCACCTT GTGAGTCAGC GAAAAGCTTT CCAACAGTCT
    ATAGAAGGGC TCATATCACT CCACCAGGAA CAGACATCCA GCCAACCAGT CATTGCCAAA
    GCTCTGCAGC AACTAAAGAA TGATGCATTG CAGCTATGCA ATAAGATAAG CAGTGCCATT
    GACAGAGTTG ATCATATGTT CACATCTGAG TTTGATGCTG AAGTTGATGA ATCTGAATCT
    GCGACTTTGC AGCAATACTA CCGGGAAGCC ATGATTCAGG CTTACAATTT TGGGTTTGAG
    TACCACAAAG AAGTTGTTCG TCTGATGTCT GGAGAATTCA GGCAGAAAAT TGGAGACAAA
    TATATCAGCT TTGCCAGAAA GTGGATGAAC TACGTGCTAA CAAAATGTGA GAGTGGCCGA
    GGCACTCGAC CCAGGTGGGC AACTCAAGGT TTTGATTTTC TTCAAGCCAT TGAACCAGCA
    TTTATTTCGG CTCTCCCAGA AGATGATTTC TTGAGTTTGC AAGCTCTGAT GAATGAGTGC
    ATTGGCCACG TTATTGGAAA ACCTCACAGT CCTGTTACAG GTCTGTACCT TGCTATTCAT
    CGGAACAGTC CCCGTCCCGT GAAAGTACCT CGATGCCATA GTGATCCACC GAATCCTCAT
    CTTATCATCC CCACTCCTGA AGGCTTCAGC ACACGGAGCG TCCCTTGCGA TGCACGGAAC
    CCTTGCAACG TTACTGTCAG TCGCCCCGCT CCCATTGGTA GTGACTCAAC TCAACCTAAA
    TCTGTCAGCA GTGCAAATGA TACCAGGGGT TCCAGTGTCC CCGAAAATGA TCGACTGGCT
    TCAATAGCAG CCGAGTTGCA GTTCAGATCC CTTAGTCGTC ACTCCAGTCC CACTGAGGAG
    CGAGAAGAAC CATCATATCC AAAAGGAGAT TCAAGTGGCT CATCCCGGCG GAGTTGGGAG
    CTCAGAACCT TAATCAACCA GACCAAAGAC ACTGTTTCCA AACAAGCACC AATAGAAGCT
    GTTCAGAAGT CTGTTCAGTT GTTTGAAGAA AGGAGATACA GAGAGATGAG GAGGAAGAAT
    ATCATTGGCC AAGTTTGCGA CACACCAAAA TCCTACGATA ATGTCATGCA TGTAGGGTTG
    AGAAAAGTGA CTTTCAAGTG GCAGAGAGGA AACAAAATTG GTGAAGGCCA ATATGGGAAA
    GTATATACCT GCATTAGTGT TGACACTGGA GAGCTGATGG CTATGAAAGA AATAAGATTT
    CAACCTAATG ATCACAAAAC CATCAAAGAA ACAGCTGATG AACTGAAGAT TTTTGAAGGC
    ATCAAACACC CTAATCTTGT TCGGTATTTT GGTGTGGAGC TCCATAGGGA AGAGATGTAC
    ATCTTCATGG AGTACTGTGA TGAGGGCACG CTGGAGGAGG TGTCAAAACT GGGCCTTCAG
    GAGCATGTCA TTAGGCTCTA CACAAAGCAG ATCACCATTG CTATCAATGT ACTACATGAA
    CACGGTATTG TTCATCGAGA CATTAAAGGA GCCAACATCT TTCTTACCTC ATCCGGACTG
    ATTAAACTGG GTGACTTTGG CTGCTCAGTA AAACTAAAGA ACAACACTCA AACAATGCCT
    GGAGAAGTGA ACAGTACATT GGGTACTGCA GCGTACATGG CTCCAGAAGT AATTACTAGA
    GCAAAAGGAG AAGGGCATGG ACGTGCAGCA GACATCTGGA GCCTGGGCTG TGTTGTTATA
    GAGATGGTCA CTGGCAAGAG GCCTTGGCAC GAATATGAGC ACAACTTCCA GATCATGTAT
    AGAGTGGGGA TGGGTCATAA ACCTCCTATT CCTGACAAAG TGAGTCCGGA GGGGAAAGAC
    TTCCTTTGCC ACTGCCTGGA AAGTGACCCA AAGATGAGGT GGACAGCCAG CCAGCTCCTC
    GACCATCCTT TTGTCAAGGT TTGCACAGAT GAAGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139811.1
    CDS238..4776
    Translation

    Target ORF information:

    RefSeq Version XM_015284325.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284325.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAAGCGTA TGTCAACAAA GCATCAGAAA AACAACGTCG GGAGACCTGC TAGCCGCTCT 
    ACTTTGAAAG AAAAGATGAG TATACCACCT CAATCCGTGC ATAAAGACAA TGGGAAAACC
    ATTGAGAACG TGGAGGAGCA CAGCTATAAG CAAGGGAAAA AAATGCGTGA CACCCTAAGG
    ACGACAGAAC GAGATCACAA GAAAAATGTG CAGTGCTCGT TTATGTTAGA CTCAGTTGGC
    GGGTCTCTGC CAAAAAAAAC AATTCCAGAT GTTGATCTCA ATAAGCCTTA CCTCAGCCTT
    GGCTGTAGCA ATGCTAAGCT TCCAGTCTCT GTGCCCATGC CAATCTCCAG AACTGCACGC
    CAGACTTCTA GGACTGATTG CCCAGCGGAC CGCTTAAAAT TCTTTGAAAC CCTGCGGCTT
    TTGTTAAAAC TTACCTCAGT CTCGAAAAAA AAGGACAGGG AGCAAAGAGG ACAGGAAAGC
    ACCTCTTCTG TCTGGCTGAA CCGATCCAAT GAACTGATCT GGCTAGAGCT GCAAGCTTGG
    CATGCTGGCC GGAGTATTAA TGACCAAGAC CTTTATCTCT ATGCAGCCCG TCAAGCTATC
    CCTGATATTA TCAATGAAAT CCTTACATTC AAAGTGAACT ATGGAAGCTT CTCCTATGTA
    AAAAATGGAG CCAGTCTCAA TGGTACGTCA GGAGAAGGAG TTTGCAAAGC AACACATGGA
    ACCAGTGCTT TGGGTTACTC AAGTTACCAC GAACACCTCC ATCGCCAGCG AATCTCATTT
    GAGCAAGTAA AGCGAATAAT GGAACTGCTA GAGTATATAG AAGCACTTTA TCCATCTCTG
    CAGGCTCTTC AAAAGGACTA TGAAAAGTAT GCTGCAAAAG ACTTCCAGGA CAGAGTGCAG
    GCACTCTGTC TGTGGTTGAA CATTACAAAA GACTTAAATC AAAAGCTAAG GATTATGGGG
    ACTGTTTTGG GCATCAAAAA TCTCTCTGAC ATTGGCTGGC CAGTGTTTGA AATTCCTTCT
    CCTCGACCGT CTAAGGACGA TGATCCAGAG GATGAAGGAG TTGATAGGGA AACTGAAGTA
    AAGGAATCCT CAGGAACATG CACAGAGGAG AGCGATGGCG AAGAACAAAT CTCTGAGGAG
    AATGTTGAGG AACTTAGACA AGCCATCAAG AACAATTTTA CTAATCAGCC AAATTTCGAC
    TTTCAGGACC ATTTTTCAAG GAAGCTTGAT AGGCTTGAAT CTGGGGATGA CTCTGCCTCT
    TGGATTATTC CAGAATGCAG TGCTGAGGCA GGCTGCAGCA AACACTGTTT GACTTCTATT
    TACAGGCCGT TTGTAGACAA AGCACTGAAG CAAATGGGGT TGAGGAAGCT AATTTTAAGA
    CTACACAAGC TAATGCATGG TTCATTGCAA AGGGCTCGTA TGGCGTTGGT AAGGAGTGAT
    TGCCCGCTGG AGTTTTCAGA ATGTCCAGAT CCCATGTTGT GTTCAGATTA CGTGCAGTTA
    TTAAATTCCC CACCTTGCTG TGAGCAAAAA TGCAGCACTG TATCATGGGA AGAGCTCAAA
    TCCATGGATT TGCCATCTTT TGAACCTGCA TTCTTGGTCC TTTGCCGAGT CCTGCTCAAT
    GTTATCCATG AATGTCTCAA GCTAAGGTTG GAACAAAGGC CTGCTGGGGA GCCATCTCTT
    TTGAGTATTA AACAGCTAGT GAGGGAGTGT AAGGAGGTTC TGAAGGGTGG CCTGTTAATG
    AAGCAATATT ACCAATTTAT GTTGCGTGAG GTGTTAGATG ACTTGCAAAA GATTGATTGC
    AACATTGATG CTTTTGAAGA GGACCTGCAT AAAATGTTGA TGGTTTACTT TGATTACATG
    CGAAGCTGGA TCCAAATGCT ACAGCAGTTA CCTCAAGCAT CTCATAGTTT AAAAAATCTC
    CTAGAGGAAG AATGGAATTT CACCAAAGAA ATAACTCCAT ATATAAGAGG AGGAGAAGCT
    CAAGCTGGAA AACTATTTTG TGATATTGCA GGAATGCTGC TGAAATCTAC AGGAATATTT
    TTAGATTCTG GACTACAAGA CAGTTGTGAT GAATTTTGGG CCAGTGCCGA TGACAGTACT
    GCATCAGATG AAATAAGGAG GTCTGTCATA GAGACCAGCC GAGCACTCAA GGAACTGTTT
    CATGAAGCTA GAGAAAGGGC CTCCAAGGCT CTTGGATTTG CTAAAATGCT GAGGAAGGAC
    CTTGAAATAG CAGCAGAGTT TGCATTGTCA GCCCCTGTCC GAGATCTTCT GAATGCTCTG
    AAAACGAAAG AGTATGTGAA GGTACAAATC CCTGGACTCG AGAATTTACA AGTATTTGTC
    CCAAATAGTA TTGCAGGTGA AAAGTCTGTG ATTTTGCAGC TCCTAAATGC GGCTGCTGGG
    AAAGATTGCT CAAAAGATTC AGATGAGGTT GCATATGAGG CCTATTTAAT AATGACCAAA
    CACAGTGACA AAGACCATGA ATTAGGTGAC ATGTGGAGTG CATGGGAGGG CCAACCAGTC
    AAAGTGGTGC CACAAGTGGA AACTGTTGAT ACGCTACGAA GCATGCAGGT TGACAACCTC
    CTTCTCGTAG TCATTCAGTC TGCCCACCTT GTGAGTCAGC GAAAAGCTTT CCAACAGTCT
    ATAGAAGGGC TCATATCACT CCACCAGGAA CAGACATCCA GCCAACCAGT CATTGCCAAA
    GCTCTGCAGC AACTAAAGAA TGATGCATTG CAGCTATGCA ATAAGATAAG CAGTGCCATT
    GACAGAGTTG ATCATATGTT CACATCTGAG TTTGATGCTG AAGTTGATGA ATCTGAATCT
    GCGACTTTGC AGCAATACTA CCGGGAAGCC ATGATTCAGG CTTACAATTT TGGGTTTGAG
    TACCACAAAG AAGTTGTTCG TCTGATGTCT GGAGAATTCA GGCAGAAAAT TGGAGACAAA
    TATATCAGCT TTGCCAGAAA GTGGATGAAC TACGTGCTAA CAAAATGTGA GAGTGGCCGA
    GGCACTCGAC CCAGGTGGGC AACTCAAGGT TTTGATTTTC TTCAAGCCAT TGAACCAGCA
    TTTATTTCGG CTCTCCCAGA AGATGATTTC TTGAGTTTGC AAGCTCTGAT GAATGAGTGC
    ATTGGCCACG TTATTGGAAA ACCTCACAGT CCTGTTACAG GTCTGTACCT TGCTATTCAT
    CGGAACAGTC CCCGTCCCGT GAAAGTACCT CGATGCCATA GTGATCCACC GAATCCTCAT
    CTTATCATCC CCACTCCTGA AGGCTTCAGC ACACGGAGCG TCCCTTGCGA TGCACGGAAC
    CCTTGCAACG TTACTGTCAG TCGCCCCGCT CCCATTGGTA GTGACTCAAC TCAACCTAAA
    TCTGTCAGCA GTGCAAATGA TACCAGGGGT TCCAGTGTCC CCGAAAATGA TCGACTGGCT
    TCAATAGCAG CCGAGTTGCA GTTCAGATCC CTTAGTCGTC ACTCCAGTCC CACTGAGGAG
    CGAGAAGAAC CATCATATCC AAAAGGAGAT TCAAGTGGCT CATCCCGGCG GAGTTGGGAG
    CTCAGAACCT TAATCAACCA GACCAAAGAC ACTGTTTCCA AACAAGCACC AATAGAAGCT
    GTTCAGAAGT CTGTTCAGTT GTTTGAAGAA AGGAGATACA GAGAGATGAG GAGGAAGAAT
    ATCATTGGCC AAGTTTGCGA CACACCAAAA TCCTACGATA ATGTCATGCA TGTAGGGTTG
    AGAAAAGTGA CTTTCAAGTG GCAGAGAGGA AACAAAATTG GTGAAGGCCA ATATGGGAAA
    GTATATACCT GCATTAGTGT TGACACTGGA GAGCTGATGG CTATGAAAGA AATAAGATTT
    CAACCTAATG ATCACAAAAC CATCAAAGAA ACAGCTGATG AACTGAAGAT TTTTGAAGGC
    ATCAAACACC CTAATCTTGT TCGGTATTTT GGTGTGGAGC TCCATAGGGA AGAGATGTAC
    ATCTTCATGG AGTACTGTGA TGAGGGCACG CTGGAGGAGG TGTCAAAACT GGGCCTTCAG
    GAGCATGTCA TTAGGCTCTA CACAAAGCAG ATCACCATTG CTATCAATGT ACTACATGAA
    CACGGTATTG TTCATCGAGA CATTAAAGGA GCCAACATCT TTCTTACCTC ATCCGGACTG
    ATTAAACTGG GTGACTTTGG CTGCTCAGTA AAACTAAAGA ACAACACTCA AACAATGCCT
    GGAGAAGTGA ACAGTACATT GGGTACTGCA GCGTACATGG CTCCAGAAGT AATTACTAGA
    GCAAAAGGAG AAGGGCATGG ACGTGCAGCA GACATCTGGA GCCTGGGCTG TGTTGTTATA
    GAGATGGTCA CTGGCAAGAG GCCTTGGCAC GAATATGAGC ACAACTTCCA GATCATGTAT
    AGAGTGGGGA TGGGTCATAA ACCTCCTATT CCTGACAAAG TGAGTCCGGA GGGGAAAGAC
    TTCCTTTGCC ACTGCCTGGA AAGTGACCCA AAGATGAGGT GGACAGCCAG CCAGCTCCTC
    GACCATCCTT TTGTCAAGGT TTGCACAGAT GAAGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33780
    Clone ID Related Accession (Same CDS sequence) XM_015284324.1 , XM_015284325.1 , XM_015284325.2 , XM_015284324.2
    Accession Version XM_015284324.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4539bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product mitogen-activated protein kinase kinase kinase 4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015284324.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAAGCGTA TGTCAACAAA GCATCAGAAA AACAACGTCG GGAGACCTGC TAGCCGCTCT 
    ACTTTGAAAG AAAAGATGAG TATACCACCT CAATCCGTGC ATAAAGACAA TGGGAAAACC
    ATTGAGAACG TGGAGGAGCA CAGCTATAAG CAAGGGAAAA AAATGCGTGA CACCCTAAGG
    ACGACAGAAC GAGATCACAA GAAAAATGTG CAGTGCTCGT TTATGTTAGA CTCAGTTGGC
    GGGTCTCTGC CAAAAAAAAC AATTCCAGAT GTTGATCTCA ATAAGCCTTA CCTCAGCCTT
    GGCTGTAGCA ATGCTAAGCT TCCAGTCTCT GTGCCCATGC CAATCTCCAG AACTGCACGC
    CAGACTTCTA GGACTGATTG CCCAGCGGAC CGCTTAAAAT TCTTTGAAAC CCTGCGGCTT
    TTGTTAAAAC TTACCTCAGT CTCGAAAAAA AAGGACAGGG AGCAAAGAGG ACAGGAAAGC
    ACCTCTTCTG TCTGGCTGAA CCGATCCAAT GAACTGATCT GGCTAGAGCT GCAAGCTTGG
    CATGCTGGCC GGAGTATTAA TGACCAAGAC CTTTATCTCT ATGCAGCCCG TCAAGCTATC
    CCTGATATTA TCAATGAAAT CCTTACATTC AAAGTGAACT ATGGAAGCTT CTCCTATGTA
    AAAAATGGAG CCAGTCTCAA TGGTACGTCA GGAGAAGGAG TTTGCAAAGC AACACATGGA
    ACCAGTGCTT TGGGTTACTC AAGTTACCAC GAACACCTCC ATCGCCAGCG AATCTCATTT
    GAGCAAGTAA AGCGAATAAT GGAACTGCTA GAGTATATAG AAGCACTTTA TCCATCTCTG
    CAGGCTCTTC AAAAGGACTA TGAAAAGTAT GCTGCAAAAG ACTTCCAGGA CAGAGTGCAG
    GCACTCTGTC TGTGGTTGAA CATTACAAAA GACTTAAATC AAAAGCTAAG GATTATGGGG
    ACTGTTTTGG GCATCAAAAA TCTCTCTGAC ATTGGCTGGC CAGTGTTTGA AATTCCTTCT
    CCTCGACCGT CTAAGGACGA TGATCCAGAG GATGAAGGAG TTGATAGGGA AACTGAAGTA
    AAGGAATCCT CAGGAACATG CACAGAGGAG AGCGATGGCG AAGAACAAAT CTCTGAGGAG
    AATGTTGAGG AACTTAGACA AGCCATCAAG AACAATTTTA CTAATCAGCC AAATTTCGAC
    TTTCAGGACC ATTTTTCAAG GAAGCTTGAT AGGCTTGAAT CTGGGGATGA CTCTGCCTCT
    TGGATTATTC CAGAATGCAG TGCTGAGGCA GGCTGCAGCA AACACTGTTT GACTTCTATT
    TACAGGCCGT TTGTAGACAA AGCACTGAAG CAAATGGGGT TGAGGAAGCT AATTTTAAGA
    CTACACAAGC TAATGCATGG TTCATTGCAA AGGGCTCGTA TGGCGTTGGT AAGGAGTGAT
    TGCCCGCTGG AGTTTTCAGA ATGTCCAGAT CCCATGTTGT GTTCAGATTA CGTGCAGTTA
    TTAAATTCCC CACCTTGCTG TGAGCAAAAA TGCAGCACTG TATCATGGGA AGAGCTCAAA
    TCCATGGATT TGCCATCTTT TGAACCTGCA TTCTTGGTCC TTTGCCGAGT CCTGCTCAAT
    GTTATCCATG AATGTCTCAA GCTAAGGTTG GAACAAAGGC CTGCTGGGGA GCCATCTCTT
    TTGAGTATTA AACAGCTAGT GAGGGAGTGT AAGGAGGTTC TGAAGGGTGG CCTGTTAATG
    AAGCAATATT ACCAATTTAT GTTGCGTGAG GTGTTAGATG ACTTGCAAAA GATTGATTGC
    AACATTGATG CTTTTGAAGA GGACCTGCAT AAAATGTTGA TGGTTTACTT TGATTACATG
    CGAAGCTGGA TCCAAATGCT ACAGCAGTTA CCTCAAGCAT CTCATAGTTT AAAAAATCTC
    CTAGAGGAAG AATGGAATTT CACCAAAGAA ATAACTCCAT ATATAAGAGG AGGAGAAGCT
    CAAGCTGGAA AACTATTTTG TGATATTGCA GGAATGCTGC TGAAATCTAC AGGAATATTT
    TTAGATTCTG GACTACAAGA CAGTTGTGAT GAATTTTGGG CCAGTGCCGA TGACAGTACT
    GCATCAGATG AAATAAGGAG GTCTGTCATA GAGACCAGCC GAGCACTCAA GGAACTGTTT
    CATGAAGCTA GAGAAAGGGC CTCCAAGGCT CTTGGATTTG CTAAAATGCT GAGGAAGGAC
    CTTGAAATAG CAGCAGAGTT TGCATTGTCA GCCCCTGTCC GAGATCTTCT GAATGCTCTG
    AAAACGAAAG AGTATGTGAA GGTACAAATC CCTGGACTCG AGAATTTACA AGTATTTGTC
    CCAAATAGTA TTGCAGGTGA AAAGTCTGTG ATTTTGCAGC TCCTAAATGC GGCTGCTGGG
    AAAGATTGCT CAAAAGATTC AGATGAGGTT GCATATGAGG CCTATTTAAT AATGACCAAA
    CACAGTGACA AAGACCATGA ATTAGGTGAC ATGTGGAGTG CATGGGAGGG CCAACCAGTC
    AAAGTGGTGC CACAAGTGGA AACTGTTGAT ACGCTACGAA GCATGCAGGT TGACAACCTC
    CTTCTCGTAG TCATTCAGTC TGCCCACCTT GTGAGTCAGC GAAAAGCTTT CCAACAGTCT
    ATAGAAGGGC TCATATCACT CCACCAGGAA CAGACATCCA GCCAACCAGT CATTGCCAAA
    GCTCTGCAGC AACTAAAGAA TGATGCATTG CAGCTATGCA ATAAGATAAG CAGTGCCATT
    GACAGAGTTG ATCATATGTT CACATCTGAG TTTGATGCTG AAGTTGATGA ATCTGAATCT
    GCGACTTTGC AGCAATACTA CCGGGAAGCC ATGATTCAGG CTTACAATTT TGGGTTTGAG
    TACCACAAAG AAGTTGTTCG TCTGATGTCT GGAGAATTCA GGCAGAAAAT TGGAGACAAA
    TATATCAGCT TTGCCAGAAA GTGGATGAAC TACGTGCTAA CAAAATGTGA GAGTGGCCGA
    GGCACTCGAC CCAGGTGGGC AACTCAAGGT TTTGATTTTC TTCAAGCCAT TGAACCAGCA
    TTTATTTCGG CTCTCCCAGA AGATGATTTC TTGAGTTTGC AAGCTCTGAT GAATGAGTGC
    ATTGGCCACG TTATTGGAAA ACCTCACAGT CCTGTTACAG GTCTGTACCT TGCTATTCAT
    CGGAACAGTC CCCGTCCCGT GAAAGTACCT CGATGCCATA GTGATCCACC GAATCCTCAT
    CTTATCATCC CCACTCCTGA AGGCTTCAGC ACACGGAGCG TCCCTTGCGA TGCACGGAAC
    CCTTGCAACG TTACTGTCAG TCGCCCCGCT CCCATTGGTA GTGACTCAAC TCAACCTAAA
    TCTGTCAGCA GTGCAAATGA TACCAGGGGT TCCAGTGTCC CCGAAAATGA TCGACTGGCT
    TCAATAGCAG CCGAGTTGCA GTTCAGATCC CTTAGTCGTC ACTCCAGTCC CACTGAGGAG
    CGAGAAGAAC CATCATATCC AAAAGGAGAT TCAAGTGGCT CATCCCGGCG GAGTTGGGAG
    CTCAGAACCT TAATCAACCA GACCAAAGAC ACTGTTTCCA AACAAGCACC AATAGAAGCT
    GTTCAGAAGT CTGTTCAGTT GTTTGAAGAA AGGAGATACA GAGAGATGAG GAGGAAGAAT
    ATCATTGGCC AAGTTTGCGA CACACCAAAA TCCTACGATA ATGTCATGCA TGTAGGGTTG
    AGAAAAGTGA CTTTCAAGTG GCAGAGAGGA AACAAAATTG GTGAAGGCCA ATATGGGAAA
    GTATATACCT GCATTAGTGT TGACACTGGA GAGCTGATGG CTATGAAAGA AATAAGATTT
    CAACCTAATG ATCACAAAAC CATCAAAGAA ACAGCTGATG AACTGAAGAT TTTTGAAGGC
    ATCAAACACC CTAATCTTGT TCGGTATTTT GGTGTGGAGC TCCATAGGGA AGAGATGTAC
    ATCTTCATGG AGTACTGTGA TGAGGGCACG CTGGAGGAGG TGTCAAAACT GGGCCTTCAG
    GAGCATGTCA TTAGGCTCTA CACAAAGCAG ATCACCATTG CTATCAATGT ACTACATGAA
    CACGGTATTG TTCATCGAGA CATTAAAGGA GCCAACATCT TTCTTACCTC ATCCGGACTG
    ATTAAACTGG GTGACTTTGG CTGCTCAGTA AAACTAAAGA ACAACACTCA AACAATGCCT
    GGAGAAGTGA ACAGTACATT GGGTACTGCA GCGTACATGG CTCCAGAAGT AATTACTAGA
    GCAAAAGGAG AAGGGCATGG ACGTGCAGCA GACATCTGGA GCCTGGGCTG TGTTGTTATA
    GAGATGGTCA CTGGCAAGAG GCCTTGGCAC GAATATGAGC ACAACTTCCA GATCATGTAT
    AGAGTGGGGA TGGGTCATAA ACCTCCTATT CCTGACAAAG TGAGTCCGGA GGGGAAAGAC
    TTCCTTTGCC ACTGCCTGGA AAGTGACCCA AAGATGAGGT GGACAGCCAG CCAGCTCCTC
    GACCATCCTT TTGTCAAGGT TTGCACAGAT GAAGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139810.1
    CDS368..4906
    Translation

    Target ORF information:

    RefSeq Version XM_015284324.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284324.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAAGCGTA TGTCAACAAA GCATCAGAAA AACAACGTCG GGAGACCTGC TAGCCGCTCT 
    ACTTTGAAAG AAAAGATGAG TATACCACCT CAATCCGTGC ATAAAGACAA TGGGAAAACC
    ATTGAGAACG TGGAGGAGCA CAGCTATAAG CAAGGGAAAA AAATGCGTGA CACCCTAAGG
    ACGACAGAAC GAGATCACAA GAAAAATGTG CAGTGCTCGT TTATGTTAGA CTCAGTTGGC
    GGGTCTCTGC CAAAAAAAAC AATTCCAGAT GTTGATCTCA ATAAGCCTTA CCTCAGCCTT
    GGCTGTAGCA ATGCTAAGCT TCCAGTCTCT GTGCCCATGC CAATCTCCAG AACTGCACGC
    CAGACTTCTA GGACTGATTG CCCAGCGGAC CGCTTAAAAT TCTTTGAAAC CCTGCGGCTT
    TTGTTAAAAC TTACCTCAGT CTCGAAAAAA AAGGACAGGG AGCAAAGAGG ACAGGAAAGC
    ACCTCTTCTG TCTGGCTGAA CCGATCCAAT GAACTGATCT GGCTAGAGCT GCAAGCTTGG
    CATGCTGGCC GGAGTATTAA TGACCAAGAC CTTTATCTCT ATGCAGCCCG TCAAGCTATC
    CCTGATATTA TCAATGAAAT CCTTACATTC AAAGTGAACT ATGGAAGCTT CTCCTATGTA
    AAAAATGGAG CCAGTCTCAA TGGTACGTCA GGAGAAGGAG TTTGCAAAGC AACACATGGA
    ACCAGTGCTT TGGGTTACTC AAGTTACCAC GAACACCTCC ATCGCCAGCG AATCTCATTT
    GAGCAAGTAA AGCGAATAAT GGAACTGCTA GAGTATATAG AAGCACTTTA TCCATCTCTG
    CAGGCTCTTC AAAAGGACTA TGAAAAGTAT GCTGCAAAAG ACTTCCAGGA CAGAGTGCAG
    GCACTCTGTC TGTGGTTGAA CATTACAAAA GACTTAAATC AAAAGCTAAG GATTATGGGG
    ACTGTTTTGG GCATCAAAAA TCTCTCTGAC ATTGGCTGGC CAGTGTTTGA AATTCCTTCT
    CCTCGACCGT CTAAGGACGA TGATCCAGAG GATGAAGGAG TTGATAGGGA AACTGAAGTA
    AAGGAATCCT CAGGAACATG CACAGAGGAG AGCGATGGCG AAGAACAAAT CTCTGAGGAG
    AATGTTGAGG AACTTAGACA AGCCATCAAG AACAATTTTA CTAATCAGCC AAATTTCGAC
    TTTCAGGACC ATTTTTCAAG GAAGCTTGAT AGGCTTGAAT CTGGGGATGA CTCTGCCTCT
    TGGATTATTC CAGAATGCAG TGCTGAGGCA GGCTGCAGCA AACACTGTTT GACTTCTATT
    TACAGGCCGT TTGTAGACAA AGCACTGAAG CAAATGGGGT TGAGGAAGCT AATTTTAAGA
    CTACACAAGC TAATGCATGG TTCATTGCAA AGGGCTCGTA TGGCGTTGGT AAGGAGTGAT
    TGCCCGCTGG AGTTTTCAGA ATGTCCAGAT CCCATGTTGT GTTCAGATTA CGTGCAGTTA
    TTAAATTCCC CACCTTGCTG TGAGCAAAAA TGCAGCACTG TATCATGGGA AGAGCTCAAA
    TCCATGGATT TGCCATCTTT TGAACCTGCA TTCTTGGTCC TTTGCCGAGT CCTGCTCAAT
    GTTATCCATG AATGTCTCAA GCTAAGGTTG GAACAAAGGC CTGCTGGGGA GCCATCTCTT
    TTGAGTATTA AACAGCTAGT GAGGGAGTGT AAGGAGGTTC TGAAGGGTGG CCTGTTAATG
    AAGCAATATT ACCAATTTAT GTTGCGTGAG GTGTTAGATG ACTTGCAAAA GATTGATTGC
    AACATTGATG CTTTTGAAGA GGACCTGCAT AAAATGTTGA TGGTTTACTT TGATTACATG
    CGAAGCTGGA TCCAAATGCT ACAGCAGTTA CCTCAAGCAT CTCATAGTTT AAAAAATCTC
    CTAGAGGAAG AATGGAATTT CACCAAAGAA ATAACTCCAT ATATAAGAGG AGGAGAAGCT
    CAAGCTGGAA AACTATTTTG TGATATTGCA GGAATGCTGC TGAAATCTAC AGGAATATTT
    TTAGATTCTG GACTACAAGA CAGTTGTGAT GAATTTTGGG CCAGTGCCGA TGACAGTACT
    GCATCAGATG AAATAAGGAG GTCTGTCATA GAGACCAGCC GAGCACTCAA GGAACTGTTT
    CATGAAGCTA GAGAAAGGGC CTCCAAGGCT CTTGGATTTG CTAAAATGCT GAGGAAGGAC
    CTTGAAATAG CAGCAGAGTT TGCATTGTCA GCCCCTGTCC GAGATCTTCT GAATGCTCTG
    AAAACGAAAG AGTATGTGAA GGTACAAATC CCTGGACTCG AGAATTTACA AGTATTTGTC
    CCAAATAGTA TTGCAGGTGA AAAGTCTGTG ATTTTGCAGC TCCTAAATGC GGCTGCTGGG
    AAAGATTGCT CAAAAGATTC AGATGAGGTT GCATATGAGG CCTATTTAAT AATGACCAAA
    CACAGTGACA AAGACCATGA ATTAGGTGAC ATGTGGAGTG CATGGGAGGG CCAACCAGTC
    AAAGTGGTGC CACAAGTGGA AACTGTTGAT ACGCTACGAA GCATGCAGGT TGACAACCTC
    CTTCTCGTAG TCATTCAGTC TGCCCACCTT GTGAGTCAGC GAAAAGCTTT CCAACAGTCT
    ATAGAAGGGC TCATATCACT CCACCAGGAA CAGACATCCA GCCAACCAGT CATTGCCAAA
    GCTCTGCAGC AACTAAAGAA TGATGCATTG CAGCTATGCA ATAAGATAAG CAGTGCCATT
    GACAGAGTTG ATCATATGTT CACATCTGAG TTTGATGCTG AAGTTGATGA ATCTGAATCT
    GCGACTTTGC AGCAATACTA CCGGGAAGCC ATGATTCAGG CTTACAATTT TGGGTTTGAG
    TACCACAAAG AAGTTGTTCG TCTGATGTCT GGAGAATTCA GGCAGAAAAT TGGAGACAAA
    TATATCAGCT TTGCCAGAAA GTGGATGAAC TACGTGCTAA CAAAATGTGA GAGTGGCCGA
    GGCACTCGAC CCAGGTGGGC AACTCAAGGT TTTGATTTTC TTCAAGCCAT TGAACCAGCA
    TTTATTTCGG CTCTCCCAGA AGATGATTTC TTGAGTTTGC AAGCTCTGAT GAATGAGTGC
    ATTGGCCACG TTATTGGAAA ACCTCACAGT CCTGTTACAG GTCTGTACCT TGCTATTCAT
    CGGAACAGTC CCCGTCCCGT GAAAGTACCT CGATGCCATA GTGATCCACC GAATCCTCAT
    CTTATCATCC CCACTCCTGA AGGCTTCAGC ACACGGAGCG TCCCTTGCGA TGCACGGAAC
    CCTTGCAACG TTACTGTCAG TCGCCCCGCT CCCATTGGTA GTGACTCAAC TCAACCTAAA
    TCTGTCAGCA GTGCAAATGA TACCAGGGGT TCCAGTGTCC CCGAAAATGA TCGACTGGCT
    TCAATAGCAG CCGAGTTGCA GTTCAGATCC CTTAGTCGTC ACTCCAGTCC CACTGAGGAG
    CGAGAAGAAC CATCATATCC AAAAGGAGAT TCAAGTGGCT CATCCCGGCG GAGTTGGGAG
    CTCAGAACCT TAATCAACCA GACCAAAGAC ACTGTTTCCA AACAAGCACC AATAGAAGCT
    GTTCAGAAGT CTGTTCAGTT GTTTGAAGAA AGGAGATACA GAGAGATGAG GAGGAAGAAT
    ATCATTGGCC AAGTTTGCGA CACACCAAAA TCCTACGATA ATGTCATGCA TGTAGGGTTG
    AGAAAAGTGA CTTTCAAGTG GCAGAGAGGA AACAAAATTG GTGAAGGCCA ATATGGGAAA
    GTATATACCT GCATTAGTGT TGACACTGGA GAGCTGATGG CTATGAAAGA AATAAGATTT
    CAACCTAATG ATCACAAAAC CATCAAAGAA ACAGCTGATG AACTGAAGAT TTTTGAAGGC
    ATCAAACACC CTAATCTTGT TCGGTATTTT GGTGTGGAGC TCCATAGGGA AGAGATGTAC
    ATCTTCATGG AGTACTGTGA TGAGGGCACG CTGGAGGAGG TGTCAAAACT GGGCCTTCAG
    GAGCATGTCA TTAGGCTCTA CACAAAGCAG ATCACCATTG CTATCAATGT ACTACATGAA
    CACGGTATTG TTCATCGAGA CATTAAAGGA GCCAACATCT TTCTTACCTC ATCCGGACTG
    ATTAAACTGG GTGACTTTGG CTGCTCAGTA AAACTAAAGA ACAACACTCA AACAATGCCT
    GGAGAAGTGA ACAGTACATT GGGTACTGCA GCGTACATGG CTCCAGAAGT AATTACTAGA
    GCAAAAGGAG AAGGGCATGG ACGTGCAGCA GACATCTGGA GCCTGGGCTG TGTTGTTATA
    GAGATGGTCA CTGGCAAGAG GCCTTGGCAC GAATATGAGC ACAACTTCCA GATCATGTAT
    AGAGTGGGGA TGGGTCATAA ACCTCCTATT CCTGACAAAG TGAGTCCGGA GGGGAAAGAC
    TTCCTTTGCC ACTGCCTGGA AAGTGACCCA AAGATGAGGT GGACAGCCAG CCAGCTCCTC
    GACCATCCTT TTGTCAAGGT TTGCACAGAT GAAGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33775
    Clone ID Related Accession (Same CDS sequence) XM_015284319.1
    Accession Version XM_015284319.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4731bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product mitogen-activated protein kinase kinase kinase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAGAGACG TCGCCGCACT GCTCCCTGCC GCCGCCGCCA TGGAGGAGCC GCCGCCGCCG 
    CCGCCGGGGA GGAAAGAGTG CAAATCAGAA GATGCTACTT GCAAGATCTC TGAATCTGAA
    CCTGAGGAGT TATCAGATGA AGCAAGTGCT GACACCTTCT ATGGAACTTC TCCTCCTAGT
    ACACCCCGCC AGATGAAGCG TATGTCAACA AAGCATCAGA AAAACAACGT CGGGAGACCT
    GCTAGCCGCT CTACTTTGAA AGAAAAGATG AGTATACCAC CTCAATCCGT GCATAAAGAC
    AATGGGAAAA CCATTGAGAA CGTGGAGGAG CACAGCTATA AGCAAGGGAA AAAAATGCGT
    GACACCCTAA GGACGACAGA ACGAGATCAC AAGAAAAATG TGCAGTGCTC GTTTATGTTA
    GACTCAGTTG GCGGGTCTCT GCCAAAAAAA ACAATTCCAG ATGTTGATCT CAATAAGCCT
    TACCTCAGCC TTGGCTGTAG CAATGCTAAG CTTCCAGTCT CTGTGCCCAT GCCAATCTCC
    AGAACTGCAC GCCAGACTTC TAGGACTGAT TGCCCAGCGG ACCGCTTAAA ATTCTTTGAA
    ACCCTGCGGC TTTTGTTAAA ACTTACCTCA GTCTCGAAAA AAAAGGACAG GGAGCAAAGA
    GGACAGGAAA GCACCTCTTC TGTCTGGCTG AACCGATCCA ATGAACTGAT CTGGCTAGAG
    CTGCAAGCTT GGCATGCTGG CCGGAGTATT AATGACCAAG ACCTTTATCT CTATGCAGCC
    CGTCAAGCTA TCCCTGATAT TATCAATGAA ATCCTTACAT TCAAAGTGAA CTATGGAAGC
    TTCTCCTATG TAAAAAATGG AGCCAGTCTC AATGGTACGT CAGGAGAAGG AGTTTGCAAA
    GCAACACATG GAACCAGTGC TTTGGGTTAC TCAAGTTACC ACGAACACCT CCATCGCCAG
    CGAATCTCAT TTGAGCAAGT AAAGCGAATA ATGGAACTGC TAGAGTATAT AGAAGCACTT
    TATCCATCTC TGCAGGCTCT TCAAAAGGAC TATGAAAAGT ATGCTGCAAA AGACTTCCAG
    GACAGAGTGC AGGCACTCTG TCTGTGGTTG AACATTACAA AAGACTTAAA TCAAAAGCTA
    AGGATTATGG GGACTGTTTT GGGCATCAAA AATCTCTCTG ACATTGGCTG GCCAGTGTTT
    GAAATTCCTT CTCCTCGACC GTCTAAGGAC GATGATCCAG AGGATGAAGG AGTTGATAGG
    GAAACTGAAG TAAAGGAATC CTCAGGAACA TGCACAGAGG AGAGCGATGG CGAAGAACAA
    ATCTCTGAGG AGAATGTTGA GGAACTTAGA CAAGCCATCA AGAACAATTT TACTAATCAG
    CCAAATTTCG ACTTTCAGGA CCATTTTTCA AGGAAGCTTG ATAGGCTTGA ATCTGGGGAT
    GACTCTGCCT CTTGGATTAT TCCAGAATGC AGTGCTGAGG CAGGCTGCAG CAAACACTGT
    TTGACTTCTA TTTACAGGCC GTTTGTAGAC AAAGCACTGA AGCAAATGGG GTTGAGGAAG
    CTAATTTTAA GACTACACAA GCTAATGCAT GGTTCATTGC AAAGGGCTCG TATGGCGTTG
    GTAAGGAGTG ATTGCCCGCT GGAGTTTTCA GAATGTCCAG ATCCCATGTT GTGTTCAGAT
    TACGTGCAGT TATTAAATTC CCCACCTTGC TGTGAGCAAA AATGCAGCAC TGTATCATGG
    GAAGAGCTCA AATCCATGGA TTTGCCATCT TTTGAACCTG CATTCTTGGT CCTTTGCCGA
    GTCCTGCTCA ATGTTATCCA TGAATGTCTC AAGCTAAGGT TGGAACAAAG GCCTGCTGGG
    GAGCCATCTC TTTTGAGTAT TAAACAGCTA GTGAGGGAGT GTAAGGAGGT TCTGAAGGGT
    GGCCTGTTAA TGAAGCAATA TTACCAATTT ATGTTGCGTG AGGTGTTAGA TGACTTGCAA
    AAGATTGATT GCAACATTGA TGCTTTTGAA GAGGACCTGC ATAAAATGTT GATGGTTTAC
    TTTGATTACA TGCGAAGCTG GATCCAAATG CTACAGCAGT TACCTCAAGC ATCTCATAGT
    TTAAAAAATC TCCTAGAGGA AGAATGGAAT TTCACCAAAG AAATAACTCC ATATATAAGA
    GGAGGAGAAG CTCAAGCTGG AAAACTATTT TGTGATATTG CAGGAATGCT GCTGAAATCT
    ACAGGAATAT TTTTAGATTC TGGACTACAA GACAGTTGTG ATGAATTTTG GGCCAGTGCC
    GATGACAGTA CTGCATCAGA TGAAATAAGG AGGTCTGTCA TAGAGACCAG CCGAGCACTC
    AAGGAACTGT TTCATGAAGC TAGAGAAAGG GCCTCCAAGG CTCTTGGATT TGCTAAAATG
    CTGAGGAAGG ACCTTGAAAT AGCAGCAGAG TTTGCATTGT CAGCCCCTGT CCGAGATCTT
    CTGAATGCTC TGAAAACGAA AGAGTATGTG AAGGTACAAA TCCCTGGACT CGAGAATTTA
    CAAGTATTTG TCCCAAATAG TATTGCAGGT GAAAAGTCTG TGATTTTGCA GCTCCTAAAT
    GCGGCTGCTG GGAAAGATTG CTCAAAAGAT TCAGATGAGG TTGCATATGA GGCCTATTTA
    ATAATGACCA AACACAGTGA CAAAGACCAT GAATTAGGTG ACATGTGGAG TGCATGGGAG
    GGCCAACCAG TCAAAGTGGT GCCACAAGTG GAAACTGTTG ATACGCTACG AAGCATGCAG
    GTTGACAACC TCCTTCTCGT AGTCATTCAG TCTGCCCACC TTGTGAGTCA GCGAAAAGCT
    TTCCAACAGT CTATAGAAGG GCTCATATCA CTCCACCAGG AACAGACATC CAGCCAACCA
    GTCATTGCCA AAGCTCTGCA GCAACTAAAG AATGATGCAT TGCAGCTATG CAATAAGATA
    AGCAGTGCCA TTGACAGAGT TGATCATATG TTCACATCTG AGTTTGATGC TGAAGTTGAT
    GAATCTGAAT CTGCGACTTT GCAGCAATAC TACCGGGAAG CCATGATTCA GGCTTACAAT
    TTTGGGTTTG AGTACCACAA AGAAGTTGTT CGTCTGATGT CTGGAGAATT CAGGCAGAAA
    ATTGGAGACA AATATATCAG CTTTGCCAGA AAGTGGATGA ACTACGTGCT AACAAAATGT
    GAGAGTGGCC GAGGCACTCG ACCCAGGTGG GCAACTCAAG GTTTTGATTT TCTTCAAGCC
    ATTGAACCAG CATTTATTTC GGCTCTCCCA GAAGATGATT TCTTGAGTTT GCAAGCTCTG
    ATGAATGAGT GCATTGGCCA CGTTATTGGA AAACCTCACA GTCCTGTTAC AGGTCTGTAC
    CTTGCTATTC ATCGGAACAG TCCCCGTCCC GTGAAAGTAC CTCGATGCCA TAGTGATCCA
    CCGAATCCTC ATCTTATCAT CCCCACTCCT GAAGGCTTCA GCACACGGAG CGTCCCTTGC
    GATGCACGGA ACCCTTGCAA CGTTACTGTC AGTCGCCCCG CTCCCATTGG TAGTGACTCA
    ACTCAACCTA AATCTGTCAG CAGTGCAAAT GATACCAGGG GTTCCAGTGT CCCCGAAAAT
    GATCGACTGG CTTCAATAGC AGCCGAGTTG CAGTTCAGAT CCCTTAGTCG TCACTCCAGT
    CCCACTGAGG AGCGAGAAGA ACCATCATAT CCAAAAGGAG ATTCAAGTGG CTCATCCCGG
    CGGAGTTGGG AGCTCAGAAC CTTAATCAAC CAGACCAAAG ACACTGTTTC CAAACAAGCA
    CCAATAGAAG CTGTTCAGAA GTCTGTTCAG TTGTTTGAAG AAAGGAGATA CAGAGAGATG
    AGGAGGAAGA ATATCATTGG CCAAGTTTGC GACACACCAA AATCCTACGA TAATGTCATG
    CATGTAGGGT TGAGAAAAGT GACTTTCAAG TGGCAGAGAG GAAACAAAAT TGGTGAAGGC
    CAATATGGGA AAGTATATAC CTGCATTAGT GTTGACACTG GAGAGCTGAT GGCTATGAAA
    GAAATAAGAT TTCAACCTAA TGATCACAAA ACCATCAAAG AAACAGCTGA TGAACTGAAG
    ATTTTTGAAG GCATCAAACA CCCTAATCTT GTTCGGTATT TTGGTGTGGA GCTCCATAGG
    GAAGAGATGT ACATCTTCAT GGAGTACTGT GATGAGGGCA CGCTGGAGGA GGTGTCAAAA
    CTGGGCCTTC AGGAGCATGT CATTAGGCTC TACACAAAGC AGATCACCAT TGCTATCAAT
    GTACTACATG AACACGGTAT TGTTCATCGA GACATTAAAG GAGCCAACAT CTTTCTTACC
    TCATCCGGAC TGATTAAACT GGGTGACTTT GGCTGCTCAG TAAAACTAAA GAACAACACT
    CAAACAATGC CTGGAGAAGT GAACAGTACA TTGGGTACTG CAGCGTACAT GGCTCCAGAA
    GTAATTACTA GAGCAAAAGG AGAAGGGCAT GGACGTGCAG CAGACATCTG GAGCCTGGGC
    TGTGTTGTTA TAGAGATGGT CACTGGCAAG AGGCCTTGGC ACGAATATGA GCACAACTTC
    CAGATCATGT ATAGAGTGGG GATGGGTCAT AAACCTCCTA TTCCTGACAA AGTGAGTCCG
    GAGGGGAAAG ACTTCCTTTG CCACTGCCTG GAAAGTGACC CAAAGATGAG GTGGACAGCC
    AGCCAGCTCC TCGACCATCC TTTTGTCAAG GTTTGCACAG ATGAAGAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139805.1
    CDS169..4899
    Translation

    Target ORF information:

    RefSeq Version XM_015284319.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284319.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAGAGACG TCGCCGCACT GCTCCCTGCC GCCGCCGCCA TGGAGGAGCC GCCGCCGCCG 
    CCGCCGGGGA GGAAAGAGTG CAAATCAGAA GATGCTACTT GCAAGATCTC TGAATCTGAA
    CCTGAGGAGT TATCAGATGA AGCAAGTGCT GACACCTTCT ATGGAACTTC TCCTCCTAGT
    ACACCCCGCC AGATGAAGCG TATGTCAACA AAGCATCAGA AAAACAACGT CGGGAGACCT
    GCTAGCCGCT CTACTTTGAA AGAAAAGATG AGTATACCAC CTCAATCCGT GCATAAAGAC
    AATGGGAAAA CCATTGAGAA CGTGGAGGAG CACAGCTATA AGCAAGGGAA AAAAATGCGT
    GACACCCTAA GGACGACAGA ACGAGATCAC AAGAAAAATG TGCAGTGCTC GTTTATGTTA
    GACTCAGTTG GCGGGTCTCT GCCAAAAAAA ACAATTCCAG ATGTTGATCT CAATAAGCCT
    TACCTCAGCC TTGGCTGTAG CAATGCTAAG CTTCCAGTCT CTGTGCCCAT GCCAATCTCC
    AGAACTGCAC GCCAGACTTC TAGGACTGAT TGCCCAGCGG ACCGCTTAAA ATTCTTTGAA
    ACCCTGCGGC TTTTGTTAAA ACTTACCTCA GTCTCGAAAA AAAAGGACAG GGAGCAAAGA
    GGACAGGAAA GCACCTCTTC TGTCTGGCTG AACCGATCCA ATGAACTGAT CTGGCTAGAG
    CTGCAAGCTT GGCATGCTGG CCGGAGTATT AATGACCAAG ACCTTTATCT CTATGCAGCC
    CGTCAAGCTA TCCCTGATAT TATCAATGAA ATCCTTACAT TCAAAGTGAA CTATGGAAGC
    TTCTCCTATG TAAAAAATGG AGCCAGTCTC AATGGTACGT CAGGAGAAGG AGTTTGCAAA
    GCAACACATG GAACCAGTGC TTTGGGTTAC TCAAGTTACC ACGAACACCT CCATCGCCAG
    CGAATCTCAT TTGAGCAAGT AAAGCGAATA ATGGAACTGC TAGAGTATAT AGAAGCACTT
    TATCCATCTC TGCAGGCTCT TCAAAAGGAC TATGAAAAGT ATGCTGCAAA AGACTTCCAG
    GACAGAGTGC AGGCACTCTG TCTGTGGTTG AACATTACAA AAGACTTAAA TCAAAAGCTA
    AGGATTATGG GGACTGTTTT GGGCATCAAA AATCTCTCTG ACATTGGCTG GCCAGTGTTT
    GAAATTCCTT CTCCTCGACC GTCTAAGGAC GATGATCCAG AGGATGAAGG AGTTGATAGG
    GAAACTGAAG TAAAGGAATC CTCAGGAACA TGCACAGAGG AGAGCGATGG CGAAGAACAA
    ATCTCTGAGG AGAATGTTGA GGAACTTAGA CAAGCCATCA AGAACAATTT TACTAATCAG
    CCAAATTTCG ACTTTCAGGA CCATTTTTCA AGGAAGCTTG ATAGGCTTGA ATCTGGGGAT
    GACTCTGCCT CTTGGATTAT TCCAGAATGC AGTGCTGAGG CAGGCTGCAG CAAACACTGT
    TTGACTTCTA TTTACAGGCC GTTTGTAGAC AAAGCACTGA AGCAAATGGG GTTGAGGAAG
    CTAATTTTAA GACTACACAA GCTAATGCAT GGTTCATTGC AAAGGGCTCG TATGGCGTTG
    GTAAGGAGTG ATTGCCCGCT GGAGTTTTCA GAATGTCCAG ATCCCATGTT GTGTTCAGAT
    TACGTGCAGT TATTAAATTC CCCACCTTGC TGTGAGCAAA AATGCAGCAC TGTATCATGG
    GAAGAGCTCA AATCCATGGA TTTGCCATCT TTTGAACCTG CATTCTTGGT CCTTTGCCGA
    GTCCTGCTCA ATGTTATCCA TGAATGTCTC AAGCTAAGGT TGGAACAAAG GCCTGCTGGG
    GAGCCATCTC TTTTGAGTAT TAAACAGCTA GTGAGGGAGT GTAAGGAGGT TCTGAAGGGT
    GGCCTGTTAA TGAAGCAATA TTACCAATTT ATGTTGCGTG AGGTGTTAGA TGACTTGCAA
    AAGATTGATT GCAACATTGA TGCTTTTGAA GAGGACCTGC ATAAAATGTT GATGGTTTAC
    TTTGATTACA TGCGAAGCTG GATCCAAATG CTACAGCAGT TACCTCAAGC ATCTCATAGT
    TTAAAAAATC TCCTAGAGGA AGAATGGAAT TTCACCAAAG AAATAACTCC ATATATAAGA
    GGAGGAGAAG CTCAAGCTGG AAAACTATTT TGTGATATTG CAGGAATGCT GCTGAAATCT
    ACAGGAATAT TTTTAGATTC TGGACTACAA GACAGTTGTG ATGAATTTTG GGCCAGTGCC
    GATGACAGTA CTGCATCAGA TGAAATAAGG AGGTCTGTCA TAGAGACCAG CCGAGCACTC
    AAGGAACTGT TTCATGAAGC TAGAGAAAGG GCCTCCAAGG CTCTTGGATT TGCTAAAATG
    CTGAGGAAGG ACCTTGAAAT AGCAGCAGAG TTTGCATTGT CAGCCCCTGT CCGAGATCTT
    CTGAATGCTC TGAAAACGAA AGAGTATGTG AAGGTACAAA TCCCTGGACT CGAGAATTTA
    CAAGTATTTG TCCCAAATAG TATTGCAGGT GAAAAGTCTG TGATTTTGCA GCTCCTAAAT
    GCGGCTGCTG GGAAAGATTG CTCAAAAGAT TCAGATGAGG TTGCATATGA GGCCTATTTA
    ATAATGACCA AACACAGTGA CAAAGACCAT GAATTAGGTG ACATGTGGAG TGCATGGGAG
    GGCCAACCAG TCAAAGTGGT GCCACAAGTG GAAACTGTTG ATACGCTACG AAGCATGCAG
    GTTGACAACC TCCTTCTCGT AGTCATTCAG TCTGCCCACC TTGTGAGTCA GCGAAAAGCT
    TTCCAACAGT CTATAGAAGG GCTCATATCA CTCCACCAGG AACAGACATC CAGCCAACCA
    GTCATTGCCA AAGCTCTGCA GCAACTAAAG AATGATGCAT TGCAGCTATG CAATAAGATA
    AGCAGTGCCA TTGACAGAGT TGATCATATG TTCACATCTG AGTTTGATGC TGAAGTTGAT
    GAATCTGAAT CTGCGACTTT GCAGCAATAC TACCGGGAAG CCATGATTCA GGCTTACAAT
    TTTGGGTTTG AGTACCACAA AGAAGTTGTT CGTCTGATGT CTGGAGAATT CAGGCAGAAA
    ATTGGAGACA AATATATCAG CTTTGCCAGA AAGTGGATGA ACTACGTGCT AACAAAATGT
    GAGAGTGGCC GAGGCACTCG ACCCAGGTGG GCAACTCAAG GTTTTGATTT TCTTCAAGCC
    ATTGAACCAG CATTTATTTC GGCTCTCCCA GAAGATGATT TCTTGAGTTT GCAAGCTCTG
    ATGAATGAGT GCATTGGCCA CGTTATTGGA AAACCTCACA GTCCTGTTAC AGGTCTGTAC
    CTTGCTATTC ATCGGAACAG TCCCCGTCCC GTGAAAGTAC CTCGATGCCA TAGTGATCCA
    CCGAATCCTC ATCTTATCAT CCCCACTCCT GAAGGCTTCA GCACACGGAG CGTCCCTTGC
    GATGCACGGA ACCCTTGCAA CGTTACTGTC AGTCGCCCCG CTCCCATTGG TAGTGACTCA
    ACTCAACCTA AATCTGTCAG CAGTGCAAAT GATACCAGGG GTTCCAGTGT CCCCGAAAAT
    GATCGACTGG CTTCAATAGC AGCCGAGTTG CAGTTCAGAT CCCTTAGTCG TCACTCCAGT
    CCCACTGAGG AGCGAGAAGA ACCATCATAT CCAAAAGGAG ATTCAAGTGG CTCATCCCGG
    CGGAGTTGGG AGCTCAGAAC CTTAATCAAC CAGACCAAAG ACACTGTTTC CAAACAAGCA
    CCAATAGAAG CTGTTCAGAA GTCTGTTCAG TTGTTTGAAG AAAGGAGATA CAGAGAGATG
    AGGAGGAAGA ATATCATTGG CCAAGTTTGC GACACACCAA AATCCTACGA TAATGTCATG
    CATGTAGGGT TGAGAAAAGT GACTTTCAAG TGGCAGAGAG GAAACAAAAT TGGTGAAGGC
    CAATATGGGA AAGTATATAC CTGCATTAGT GTTGACACTG GAGAGCTGAT GGCTATGAAA
    GAAATAAGAT TTCAACCTAA TGATCACAAA ACCATCAAAG AAACAGCTGA TGAACTGAAG
    ATTTTTGAAG GCATCAAACA CCCTAATCTT GTTCGGTATT TTGGTGTGGA GCTCCATAGG
    GAAGAGATGT ACATCTTCAT GGAGTACTGT GATGAGGGCA CGCTGGAGGA GGTGTCAAAA
    CTGGGCCTTC AGGAGCATGT CATTAGGCTC TACACAAAGC AGATCACCAT TGCTATCAAT
    GTACTACATG AACACGGTAT TGTTCATCGA GACATTAAAG GAGCCAACAT CTTTCTTACC
    TCATCCGGAC TGATTAAACT GGGTGACTTT GGCTGCTCAG TAAAACTAAA GAACAACACT
    CAAACAATGC CTGGAGAAGT GAACAGTACA TTGGGTACTG CAGCGTACAT GGCTCCAGAA
    GTAATTACTA GAGCAAAAGG AGAAGGGCAT GGACGTGCAG CAGACATCTG GAGCCTGGGC
    TGTGTTGTTA TAGAGATGGT CACTGGCAAG AGGCCTTGGC ACGAATATGA GCACAACTTC
    CAGATCATGT ATAGAGTGGG GATGGGTCAT AAACCTCCTA TTCCTGACAA AGTGAGTCCG
    GAGGGGAAAG ACTTCCTTTG CCACTGCCTG GAAAGTGACC CAAAGATGAG GTGGACAGCC
    AGCCAGCTCC TCGACCATCC TTTTGTCAAG GTTTGCACAG ATGAAGAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33776
    Clone ID Related Accession (Same CDS sequence) XM_015284320.1
    Accession Version XM_015284320.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4719bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product mitogen-activated protein kinase kinase kinase 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAGAGACG TCGCCGCACT GCTCCCTGCC GCCGCCGCCA TGGAGGAGCC GCCGCCGCCG 
    CCGCCGGGGA GGAAAGAGTG CAAATCAGAA GATGCTACTT GCAAGATCTC TGAATCTGAA
    CCTGAGGAGT TATCAGATGA AGCAAGTGCT GACACCTTCT ATGGAACTTC TCCTCCTAGT
    ACACCCCGCC AGATGAAGCG TATGTCAACA AAGCATCAGA AAAACAACGT CGGGAGACCT
    GCTAGCCGCT CTACTTTGAA AGAAAAGATG AGTATACCAC CTCAATCCGT GCATAAAGAC
    AATGGGAAAA CCATTGAGAA CGTGGAGGAG CACAGCTATA AGCAAGGGAA AAAAATGCGT
    GACACCCTAA GGACGACAGA ACGAGATCAC AAGAAAAATG TGCAGTGCTC GTTTATGTTA
    GACTCAGTTG GCGGGTCTCT GCCAAAAAAA ACAATTCCAG ATGTTGATCT CAATAAGCCT
    TACCTCAGCC TTGGCTGTAG CAATGCTAAG CTTCCAGTCT CTGTGCCCAT GCCAATCTCC
    AGAACTGCAC GCCAGACTTC TAGGACTGAT TGCCCAGCGG ACCGCTTAAA ATTCTTTGAA
    ACCCTGCGGC TTTTGTTAAA ACTTACCTCA GTCTCGAAAA AAAAGGACAG GGAGCAAAGA
    GGACAGGAAA GCACCTCTTC TGTCTGGCTG AACCGATCCA ATGAACTGAT CTGGCTAGAG
    CTGCAAGCTT GGCATGCTGG CCGGAGTATT AATGACCAAG ACCTTTATCT CTATGCAGCC
    CGTCAAGCTA TCCCTGATAT TATCAATGAA ATCCTTACAT TCAAAGTGAA CTATGGAAGC
    TTCTCCTATG TAAAAAATGG AGCCAGTCTC AATGGTACGT CAGGAGAAGG AGTTTGCAAA
    GCAACACATG GAACCAGTGC TTTGGGTTAC TCAAGTTACC ACGAACACCT CCATCGCCAG
    CGAATCTCAT TTGAGCAAGT AAAGCGAATA ATGGAACTGC TAGAGTATAT AGAAGCACTT
    TATCCATCTC TGCAGGCTCT TCAAAAGGAC TATGAAAAGT ATGCTGCAAA AGACTTCCAG
    GACAGAGTGC AGGCACTCTG TCTGTGGTTG AACATTACAA AAGACTTAAA TCAAAAGCTA
    AGGATTATGG GGACTGTTTT GGGCATCAAA AATCTCTCTG ACATTGGCTG GCCAGTGTTT
    GAAATTCCTT CTCCTCGACC GTCTAAGGAC GATGATCCAG AGGATGAAGG AGTTGATAGG
    GAAACTGAAG TAAAGGAATC CTCAGGAACA TGCACAGAGG AGAGCGATGG CGAAGAACAA
    ATCTCTGAGG AGAATGTTGA GGAACTTAGA CAAGCCATCA AGAACAATTT TACTAATCAG
    CCAAATTTCG ACTTTCAGGA CCATTTTTCA AGGAAGCTTG ATAGGCTTGA ATCTGGGGAT
    GACTCTGCCT CTTGGATTAT TCCAGAATGC AGTGCTGAGG CAGGCTGCAG CAAACACTGT
    TTGACTTCTA TTTACAGGCC GTTTGTAGAC AAAGCACTGA AGCAAATGGG GTTGAGGAAG
    CTAATTTTAA GACTACACAA GCTAATGCAT GGTTCATTGC AAAGGGCTCG TATGGCGTTG
    GTAAGGAGTG ATTGCCCGCT GGAGTTTTCA GAATGTCCAG ATCCCATGTT GTGTTCAGAT
    TACGTGCAGT TATTAAATTC CCCACCTTGC TGTGAGCAAA AATGCAGCAC TGTATCATGG
    GAAGAGCTCA AATCCATGGA TTTGCCATCT TTTGAACCTG CATTCTTGGT CCTTTGCCGA
    GTCCTGCTCA ATGTTATCCA TGAATGTCTC AAGCTAAGGT TGGAACAAAG GCCTGCTGGG
    GAGCCATCTC TTTTGAGTAT TAAACAGCTA GTGAGGGAGT GTAAGGAGGT TCTGAAGGGT
    GGCCTGTTAA TGAAGCAATA TTACCAATTT ATGTTGCGTG AGGTGTTAGA TGACTTGCAA
    AAGATTGATT GCAACATTGA TGCTTTTGAA GAGGACCTGC ATAAAATGTT GATGGTTTAC
    TTTGATTACA TGCGAAGCTG GATCCAAATG CTACAGCAGT TACCTCAAGC ATCTCATAGT
    TTAAAAAATC TCCTAGAGGA AGAATGGAAT TTCACCAAAG AAATAACTCC ATATATAAGA
    GGAGGAGAAG CTCAAGCTGG AAAACTATTT TGTGATATTG CAGGAATGCT GCTGAAATCT
    ACAGGAATAT TTTTAGATTC TGGACTACAA GACAGTTGTG ATGAATTTTG GGCCAGTGCC
    GATGACAGTA CTGCATCAGA TGAAATAAGG AGGTCTGTCA TAGAGACCAG CCGAGCACTC
    AAGGAACTGT TTCATGAAGC TAGAGAAAGG GCCTCCAAGG CTCTTGGATT TGCTAAAATG
    CTGAGGAAGG ACCTTGAAAT AGCAGCAGAG TTTGCATTGT CAGCCCCTGT CCGAGATCTT
    CTGAATGCTC TGAAAACGAA AGAGTATGTG AAGGTACAAA TCCCTGGACT CGAGAATTTA
    CAAGTATTTG TCCCAAATAG TATTGCAGGT GAAAAGTCTG TGATTTTGCA GCTCCTAAAT
    GCGGCTGCTG GGAAAGATTG CTCAAAAGAT TCAGATGAGG TTGCATATGA GGCCTATTTA
    ATAATGACCA AACACAGTGA CAAAGACCAT GAATTAGGTG ACATGTGGAG TGCATGGGAG
    GGCCAACCAG TCAAAGTGGT GCCACAAGTG GAAACTGTTG ATACGCTACG AAGCATGCAG
    GTTGACAACC TCCTTCTCGT AGTCATTCAG TCTGCCCACC TTGTGAGTCA GCGAAAAGCT
    TTCCAACAGT CTATAGAAGG GCTCATATCA CTCCACCAGG AACAGACATC CAGCCAACCA
    GTCATTGCCA AAGCTCTGCA GCAACTAAAG AATGATGCAT TGCAGCTATG CAATAAGATA
    AGCAGTGCCA TTGACAGAGT TGATCATATG TTCACATCTG AGTTTGATGC TGAAGTTGAT
    GAATCTGAAT CTGCGACTTT GCAGCAATAC TACCGGGAAG CCATGATTCA GGCTTACAAT
    TTTGGGTTTG AGTACCACAA AGAAGTTGTT CGTCTGATGT CTGGAGAATT CAGGCAGAAA
    ATTGGAGACA AATATATCAG CTTTGCCAGA AAGTGGATGA ACTACGTGCT AACAAAATGT
    GAGAGTGGCC GAGGCACTCG ACCCAGGTGG GCAACTCAAG GTTTTGATTT TCTTCAAGCC
    ATTGAACCAG CATTTATTTC GGCTCTCCCA GAAGATGATT TCTTGAGTTT GCAAGCTCTG
    ATGAATGAGT GCATTGGCCA CGTTATTGGA AAACCTCACA GTCCTGTTAC AGCTATTCAT
    CGGAACAGTC CCCGTCCCGT GAAAGTACCT CGATGCCATA GTGATCCACC GAATCCTCAT
    CTTATCATCC CCACTCCTGA AGGCTTCAGC ACACGGAGCG TCCCTTGCGA TGCACGGAAC
    CCTTGCAACG TTACTGTCAG TCGCCCCGCT CCCATTGGTA GTGACTCAAC TCAACCTAAA
    TCTGTCAGCA GTGCAAATGA TACCAGGGGT TCCAGTGTCC CCGAAAATGA TCGACTGGCT
    TCAATAGCAG CCGAGTTGCA GTTCAGATCC CTTAGTCGTC ACTCCAGTCC CACTGAGGAG
    CGAGAAGAAC CATCATATCC AAAAGGAGAT TCAAGTGGCT CATCCCGGCG GAGTTGGGAG
    CTCAGAACCT TAATCAACCA GACCAAAGAC ACTGTTTCCA AACAAGCACC AATAGAAGCT
    GTTCAGAAGT CTGTTCAGTT GTTTGAAGAA AGGAGATACA GAGAGATGAG GAGGAAGAAT
    ATCATTGGCC AAGTTTGCGA CACACCAAAA TCCTACGATA ATGTCATGCA TGTAGGGTTG
    AGAAAAGTGA CTTTCAAGTG GCAGAGAGGA AACAAAATTG GTGAAGGCCA ATATGGGAAA
    GTATATACCT GCATTAGTGT TGACACTGGA GAGCTGATGG CTATGAAAGA AATAAGATTT
    CAACCTAATG ATCACAAAAC CATCAAAGAA ACAGCTGATG AACTGAAGAT TTTTGAAGGC
    ATCAAACACC CTAATCTTGT TCGGTATTTT GGTGTGGAGC TCCATAGGGA AGAGATGTAC
    ATCTTCATGG AGTACTGTGA TGAGGGCACG CTGGAGGAGG TGTCAAAACT GGGCCTTCAG
    GAGCATGTCA TTAGGCTCTA CACAAAGCAG ATCACCATTG CTATCAATGT ACTACATGAA
    CACGGTATTG TTCATCGAGA CATTAAAGGA GCCAACATCT TTCTTACCTC ATCCGGACTG
    ATTAAACTGG GTGACTTTGG CTGCTCAGTA AAACTAAAGA ACAACACTCA AACAATGCCT
    GGAGAAGTGA ACAGTACATT GGGTACTGCA GCGTACATGG CTCCAGAAGT AATTACTAGA
    GCAAAAGGAG AAGGGCATGG ACGTGCAGCA GACATCTGGA GCCTGGGCTG TGTTGTTATA
    GAGATGGTCA CTGGCAAGAG GCCTTGGCAC GAATATGAGC ACAACTTCCA GATCATGTAT
    AGAGTGGGGA TGGGTCATAA ACCTCCTATT CCTGACAAAG TGAGTCCGGA GGGGAAAGAC
    TTCCTTTGCC ACTGCCTGGA AAGTGACCCA AAGATGAGGT GGACAGCCAG CCAGCTCCTC
    GACCATCCTT TTGTCAAGGT TTGCACAGAT GAAGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139806.1
    CDS169..4887
    Translation

    Target ORF information:

    RefSeq Version XM_015284320.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284320.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAGAGACG TCGCCGCACT GCTCCCTGCC GCCGCCGCCA TGGAGGAGCC GCCGCCGCCG 
    CCGCCGGGGA GGAAAGAGTG CAAATCAGAA GATGCTACTT GCAAGATCTC TGAATCTGAA
    CCTGAGGAGT TATCAGATGA AGCAAGTGCT GACACCTTCT ATGGAACTTC TCCTCCTAGT
    ACACCCCGCC AGATGAAGCG TATGTCAACA AAGCATCAGA AAAACAACGT CGGGAGACCT
    GCTAGCCGCT CTACTTTGAA AGAAAAGATG AGTATACCAC CTCAATCCGT GCATAAAGAC
    AATGGGAAAA CCATTGAGAA CGTGGAGGAG CACAGCTATA AGCAAGGGAA AAAAATGCGT
    GACACCCTAA GGACGACAGA ACGAGATCAC AAGAAAAATG TGCAGTGCTC GTTTATGTTA
    GACTCAGTTG GCGGGTCTCT GCCAAAAAAA ACAATTCCAG ATGTTGATCT CAATAAGCCT
    TACCTCAGCC TTGGCTGTAG CAATGCTAAG CTTCCAGTCT CTGTGCCCAT GCCAATCTCC
    AGAACTGCAC GCCAGACTTC TAGGACTGAT TGCCCAGCGG ACCGCTTAAA ATTCTTTGAA
    ACCCTGCGGC TTTTGTTAAA ACTTACCTCA GTCTCGAAAA AAAAGGACAG GGAGCAAAGA
    GGACAGGAAA GCACCTCTTC TGTCTGGCTG AACCGATCCA ATGAACTGAT CTGGCTAGAG
    CTGCAAGCTT GGCATGCTGG CCGGAGTATT AATGACCAAG ACCTTTATCT CTATGCAGCC
    CGTCAAGCTA TCCCTGATAT TATCAATGAA ATCCTTACAT TCAAAGTGAA CTATGGAAGC
    TTCTCCTATG TAAAAAATGG AGCCAGTCTC AATGGTACGT CAGGAGAAGG AGTTTGCAAA
    GCAACACATG GAACCAGTGC TTTGGGTTAC TCAAGTTACC ACGAACACCT CCATCGCCAG
    CGAATCTCAT TTGAGCAAGT AAAGCGAATA ATGGAACTGC TAGAGTATAT AGAAGCACTT
    TATCCATCTC TGCAGGCTCT TCAAAAGGAC TATGAAAAGT ATGCTGCAAA AGACTTCCAG
    GACAGAGTGC AGGCACTCTG TCTGTGGTTG AACATTACAA AAGACTTAAA TCAAAAGCTA
    AGGATTATGG GGACTGTTTT GGGCATCAAA AATCTCTCTG ACATTGGCTG GCCAGTGTTT
    GAAATTCCTT CTCCTCGACC GTCTAAGGAC GATGATCCAG AGGATGAAGG AGTTGATAGG
    GAAACTGAAG TAAAGGAATC CTCAGGAACA TGCACAGAGG AGAGCGATGG CGAAGAACAA
    ATCTCTGAGG AGAATGTTGA GGAACTTAGA CAAGCCATCA AGAACAATTT TACTAATCAG
    CCAAATTTCG ACTTTCAGGA CCATTTTTCA AGGAAGCTTG ATAGGCTTGA ATCTGGGGAT
    GACTCTGCCT CTTGGATTAT TCCAGAATGC AGTGCTGAGG CAGGCTGCAG CAAACACTGT
    TTGACTTCTA TTTACAGGCC GTTTGTAGAC AAAGCACTGA AGCAAATGGG GTTGAGGAAG
    CTAATTTTAA GACTACACAA GCTAATGCAT GGTTCATTGC AAAGGGCTCG TATGGCGTTG
    GTAAGGAGTG ATTGCCCGCT GGAGTTTTCA GAATGTCCAG ATCCCATGTT GTGTTCAGAT
    TACGTGCAGT TATTAAATTC CCCACCTTGC TGTGAGCAAA AATGCAGCAC TGTATCATGG
    GAAGAGCTCA AATCCATGGA TTTGCCATCT TTTGAACCTG CATTCTTGGT CCTTTGCCGA
    GTCCTGCTCA ATGTTATCCA TGAATGTCTC AAGCTAAGGT TGGAACAAAG GCCTGCTGGG
    GAGCCATCTC TTTTGAGTAT TAAACAGCTA GTGAGGGAGT GTAAGGAGGT TCTGAAGGGT
    GGCCTGTTAA TGAAGCAATA TTACCAATTT ATGTTGCGTG AGGTGTTAGA TGACTTGCAA
    AAGATTGATT GCAACATTGA TGCTTTTGAA GAGGACCTGC ATAAAATGTT GATGGTTTAC
    TTTGATTACA TGCGAAGCTG GATCCAAATG CTACAGCAGT TACCTCAAGC ATCTCATAGT
    TTAAAAAATC TCCTAGAGGA AGAATGGAAT TTCACCAAAG AAATAACTCC ATATATAAGA
    GGAGGAGAAG CTCAAGCTGG AAAACTATTT TGTGATATTG CAGGAATGCT GCTGAAATCT
    ACAGGAATAT TTTTAGATTC TGGACTACAA GACAGTTGTG ATGAATTTTG GGCCAGTGCC
    GATGACAGTA CTGCATCAGA TGAAATAAGG AGGTCTGTCA TAGAGACCAG CCGAGCACTC
    AAGGAACTGT TTCATGAAGC TAGAGAAAGG GCCTCCAAGG CTCTTGGATT TGCTAAAATG
    CTGAGGAAGG ACCTTGAAAT AGCAGCAGAG TTTGCATTGT CAGCCCCTGT CCGAGATCTT
    CTGAATGCTC TGAAAACGAA AGAGTATGTG AAGGTACAAA TCCCTGGACT CGAGAATTTA
    CAAGTATTTG TCCCAAATAG TATTGCAGGT GAAAAGTCTG TGATTTTGCA GCTCCTAAAT
    GCGGCTGCTG GGAAAGATTG CTCAAAAGAT TCAGATGAGG TTGCATATGA GGCCTATTTA
    ATAATGACCA AACACAGTGA CAAAGACCAT GAATTAGGTG ACATGTGGAG TGCATGGGAG
    GGCCAACCAG TCAAAGTGGT GCCACAAGTG GAAACTGTTG ATACGCTACG AAGCATGCAG
    GTTGACAACC TCCTTCTCGT AGTCATTCAG TCTGCCCACC TTGTGAGTCA GCGAAAAGCT
    TTCCAACAGT CTATAGAAGG GCTCATATCA CTCCACCAGG AACAGACATC CAGCCAACCA
    GTCATTGCCA AAGCTCTGCA GCAACTAAAG AATGATGCAT TGCAGCTATG CAATAAGATA
    AGCAGTGCCA TTGACAGAGT TGATCATATG TTCACATCTG AGTTTGATGC TGAAGTTGAT
    GAATCTGAAT CTGCGACTTT GCAGCAATAC TACCGGGAAG CCATGATTCA GGCTTACAAT
    TTTGGGTTTG AGTACCACAA AGAAGTTGTT CGTCTGATGT CTGGAGAATT CAGGCAGAAA
    ATTGGAGACA AATATATCAG CTTTGCCAGA AAGTGGATGA ACTACGTGCT AACAAAATGT
    GAGAGTGGCC GAGGCACTCG ACCCAGGTGG GCAACTCAAG GTTTTGATTT TCTTCAAGCC
    ATTGAACCAG CATTTATTTC GGCTCTCCCA GAAGATGATT TCTTGAGTTT GCAAGCTCTG
    ATGAATGAGT GCATTGGCCA CGTTATTGGA AAACCTCACA GTCCTGTTAC AGCTATTCAT
    CGGAACAGTC CCCGTCCCGT GAAAGTACCT CGATGCCATA GTGATCCACC GAATCCTCAT
    CTTATCATCC CCACTCCTGA AGGCTTCAGC ACACGGAGCG TCCCTTGCGA TGCACGGAAC
    CCTTGCAACG TTACTGTCAG TCGCCCCGCT CCCATTGGTA GTGACTCAAC TCAACCTAAA
    TCTGTCAGCA GTGCAAATGA TACCAGGGGT TCCAGTGTCC CCGAAAATGA TCGACTGGCT
    TCAATAGCAG CCGAGTTGCA GTTCAGATCC CTTAGTCGTC ACTCCAGTCC CACTGAGGAG
    CGAGAAGAAC CATCATATCC AAAAGGAGAT TCAAGTGGCT CATCCCGGCG GAGTTGGGAG
    CTCAGAACCT TAATCAACCA GACCAAAGAC ACTGTTTCCA AACAAGCACC AATAGAAGCT
    GTTCAGAAGT CTGTTCAGTT GTTTGAAGAA AGGAGATACA GAGAGATGAG GAGGAAGAAT
    ATCATTGGCC AAGTTTGCGA CACACCAAAA TCCTACGATA ATGTCATGCA TGTAGGGTTG
    AGAAAAGTGA CTTTCAAGTG GCAGAGAGGA AACAAAATTG GTGAAGGCCA ATATGGGAAA
    GTATATACCT GCATTAGTGT TGACACTGGA GAGCTGATGG CTATGAAAGA AATAAGATTT
    CAACCTAATG ATCACAAAAC CATCAAAGAA ACAGCTGATG AACTGAAGAT TTTTGAAGGC
    ATCAAACACC CTAATCTTGT TCGGTATTTT GGTGTGGAGC TCCATAGGGA AGAGATGTAC
    ATCTTCATGG AGTACTGTGA TGAGGGCACG CTGGAGGAGG TGTCAAAACT GGGCCTTCAG
    GAGCATGTCA TTAGGCTCTA CACAAAGCAG ATCACCATTG CTATCAATGT ACTACATGAA
    CACGGTATTG TTCATCGAGA CATTAAAGGA GCCAACATCT TTCTTACCTC ATCCGGACTG
    ATTAAACTGG GTGACTTTGG CTGCTCAGTA AAACTAAAGA ACAACACTCA AACAATGCCT
    GGAGAAGTGA ACAGTACATT GGGTACTGCA GCGTACATGG CTCCAGAAGT AATTACTAGA
    GCAAAAGGAG AAGGGCATGG ACGTGCAGCA GACATCTGGA GCCTGGGCTG TGTTGTTATA
    GAGATGGTCA CTGGCAAGAG GCCTTGGCAC GAATATGAGC ACAACTTCCA GATCATGTAT
    AGAGTGGGGA TGGGTCATAA ACCTCCTATT CCTGACAAAG TGAGTCCGGA GGGGAAAGAC
    TTCCTTTGCC ACTGCCTGGA AAGTGACCCA AAGATGAGGT GGACAGCCAG CCAGCTCCTC
    GACCATCCTT TTGTCAAGGT TTGCACAGAT GAAGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33778
    Clone ID Related Accession (Same CDS sequence) XM_015284322.1
    Accession Version XM_015284322.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4614bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product mitogen-activated protein kinase kinase kinase 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGAGACG TCGCCGCACT GCTCCCTGCC GCCGCCGCCA TGGAGGAGCC GCCGCCGCCG 
    CCGCCGGGGA GGAAAGAGTG CAAATCAGAA GATGCTACTT GCAAGATCTC TGAATCTGAA
    CCTGAGGAGT TATCAGATGA AGCAAGTGCT GACACCTTCT ATGGAACTTC TCCTCCTAGT
    ACACCCCGCC AGATGAAGCG TATGTCAACA AAGCATCAGA AAAACAACGT CGGGAGACCT
    GCTAGCCGCT CTACTTTGAA AGAAAAGATG AGTATACCAC CTCAATCCGT GCATAAAGAC
    AATGGGAAAA CCATTGAGAA CGTGGAGGAG CACAGCTATA AGCAAGGGAA AAAAATGCGT
    GACACCCTAA GGACGACAGA ACGAGATCAC AAGAAAAATG TGCAGTGCTC GTTTATGTTA
    GACTCAGTTG GCGGGTCTCT GCCAAAAAAA ACAATTCCAG ATGTTGATCT CAATAAGCCT
    TACCTCAGCC TTGGCTGTAG CAATGCTAAG CTTCCAGTCT CTGTGCCCAT GCCAATCTCC
    AGAACTGCAC GCCAGACTTC TAGGACTGAT TGCCCAGCGG ACCGCTTAAA ATTCTTTGAA
    ACCCTGCGGC TTTTGTTAAA ACTTACCTCA GTCTCGAAAA AAAAGGACAG GGAGCAAAGA
    GGACAGGAAA GCACCTCTTC TGTCTGGCTG AACCGATCCA ATGAACTGAT CTGGCTAGAG
    CTGCAAGCTT GGCATGCTGG CCGGAGTATT AATGACCAAG ACCTTTATCT CTATGCAGCC
    CGTCAAGCTA TCCCTGATAT TATCAATGAA ATCCTTACAT TCAAAGTGAA CTATGGAAGC
    TTCTCCTATG TAAAAAATGG AGCCAGTCTC AATGGTACGT CAGGAGAAGG AGTTTGCAAA
    GCAACACATG GAACCAGTGC TTTGGGTTAC TCAAGTTACC ACGAACACCT CCATCGCCAG
    CGAATCTCAT TTGAGCAAGT AAAGCGAATA ATGGAACTGC TAGAGTATAT AGAAGCACTT
    TATCCATCTC TGCAGGCTCT TCAAAAGGAC TATGAAAAGT ATGCTGCAAA AGACTTCCAG
    GACAGAGTGC AGGCACTCTG TCTGTGGTTG AACATTACAA AAGACTTAAA TCAAAAGCTA
    AGGATTATGG GGACTGTTTT GGGCATCAAA AATCTCTCTG ACATTGGCTG GCCAGTGTTT
    GAAATTCCTT CTCCTCGACC GTCTAAGGAC GATGATCCAG AGGATGAAGG AGTTGATAGG
    GAAACTGAAG TAAAGGAATC CTCAGGAACA TGCACAGAGG AGAGCGATGG CGAAGAACAA
    ATCTCTGAGG AGAATGTTGA GGAACTTAGA CAAGCCATCA AGAACAATTT TACTAATCAG
    CCAAATTTCG ACTTTCAGGA CCATTTTTCA AGGAAGCTTG ATAGGCTTGA ATCTGGGGAT
    GACTCTGCCT CTTGGATTAT TCCAGAATGC AGTGCTGAGG CAGGCTGCAG CAAACACTGT
    TTGACTTCTA TTTACAGGCC GTTTGTAGAC AAAGCACTGA AGCAAATGGG GTTGAGGAAG
    CTAATTTTAA GACTACACAA GCTAATGCAT GGTTCATTGC AAAGGGCTCG TATGGCGTTG
    GTAAGGAGTG ATTGCCCGCT GGAGTTTTCA GAATGTCCAG ATCCCATGTT GTGTTCAGAT
    TACGTGCAGT TATTAAATTC CCCACCTTGC TGTGAGCAAA AATGCAGCAC TGTATCATGG
    GAAGAGCTCA AATCCATGGA TTTGCCATCT TTTGAACCTG CATTCTTGGT CCTTTGCCGA
    GTCCTGCTCA ATGTTATCCA TGAATGTCTC AAGCTAAGGT TGGAACAAAG GCCTGCTGGG
    GAGCCATCTC TTTTGAGTAT TAAACAGCTA GTGAGGGAGT GTAAGGAGGT TCTGAAGGGT
    GGCCTGTTAA TGAAGCAATA TTACCAATTT ATGTTGCGTG AGGTGTTAGA TGACTTGCAA
    AAGATTGATT GCAACATTGA TGCTTTTGAA GAGGACCTGC ATAAAATGTT GATGGTTTAC
    TTTGATTACA TGCGAAGCTG GATCCAAATG CTACAGCAGT TACCTCAAGC ATCTCATAGT
    TTAAAAAATC TCCTAGAGGA AGAATGGAAT TTCACCAAAG AAATAACTCC ATATATAAGA
    GGAGGAGAAG CTCAAGCTGG AAAACTATTT TGTGATATTG CAGGAATGCT GCTGAAATCT
    ACAGGAATAT TTTTAGATTC TGGACTACAA GACAGTTGTG ATGAATTTTG GGCCAGTGCC
    GATGACAGTA CTGCATCAGA TGAAATAAGG AGGTCTGTCA TAGAGACCAG CCGAGCACTC
    AAGGAACTGT TTCATGAAGC TAGAGAAAGG GCCTCCAAGG CTCTTGGATT TGCTAAAATG
    CTGAGGAAGG ACCTTGAAAT AGCAGCAGAG TTTGCATTGT CAGCCCCTGT CCGAGATCTT
    CTGAATGCTC TGAAAACGAA AGAGTATGTG AAGGTACAAA TCCCTGGACT CGAGAATTTA
    CAAGTATTTG TCCCAAATAG TATTGCAGGT GAAAAGTCTG TGATTTTGCA GCTCCTAAAT
    GCGGCTGCTG GGAAAGATTG CTCAAAAGAT TCAGATGAGG TTGCATATGA GGCCTATTTA
    ATAATGACCA AACACAGTGA CAAAGACCAT GAATTAGGTG ACATGTGGAG TGCATGGGAG
    GGCCAACCAG TCAAAGTGGT GCCACAAGTG GAAACTGTTG ATACGCTACG AAGCATGCAG
    GTTGACAACC TCCTTCTCGT AGTCATTCAG TCTGCCCACC TTGTGAGTCA GCGAAAAGCT
    TTCCAACAGT CTATAGAAGG GCTCATATCA CTCCACCAGG AACAGACATC CAGCCAACCA
    GTCATTGCCA AAGCTCTGCA GCAACTAAAG AATGATGCAT TGCAGCTATG CAATAAGATA
    AGCAGTGCCA TTGACAGAGT TGATCATATG TTCACATCTG AGTTTGATGC TGAAGTTGAT
    GAATCTGAAT CTGCGACTTT GCAGCAATAC TACCGGGAAG CCATGATTCA GGCTTACAAT
    TTTGGGTTTG AGTACCACAA AGAAGTTGTT CGTCTGATGT CTGGAGAATT CAGGCAGAAA
    ATTGGAGACA AATATATCAG CTTTGCCAGA AAGTGGATGA ACTACGTGCT AACAAAATGT
    GAGAGTGGCC GAGGCACTCG ACCCAGGTGG GCAACTCAAG GTTTTGATTT TCTTCAAGCC
    ATTGAACCAG CATTTATTTC GGCTCTCCCA GAAGATGATT TCTTGAGTTT GCAAGCTCTG
    ATGAATGAGT GCATTGGCCA CGTTATTGGA AAACCTCACA GTCCTGTTAC AGGTCTGTAC
    CTTGCTATTC ATCGGAACAG TCCCCGTCCC GTGAAAGTAC CTCGATGCCA TAGTGATCCA
    CCGAATCCTC ATCTTATCAT CCCCACTCCT GAAGGCTTCA GGGGTTCCAG TGTCCCCGAA
    AATGATCGAC TGGCTTCAAT AGCAGCCGAG TTGCAGTTCA GATCCCTTAG TCGTCACTCC
    AGTCCCACTG AGGAGCGAGA AGAACCATCA TATCCAAAAG GAGATTCAAG TGGCTCATCC
    CGGCGGAGTT GGGAGCTCAG AACCTTAATC AACCAGACCA AAGACACTGT TTCCAAACAA
    GCACCAATAG AAGCTGTTCA GAAGTCTGTT CAGTTGTTTG AAGAAAGGAG ATACAGAGAG
    ATGAGGAGGA AGAATATCAT TGGCCAAGTT TGCGACACAC CAAAATCCTA CGATAATGTC
    ATGCATGTAG GGTTGAGAAA AGTGACTTTC AAGTGGCAGA GAGGAAACAA AATTGGTGAA
    GGCCAATATG GGAAAGTATA TACCTGCATT AGTGTTGACA CTGGAGAGCT GATGGCTATG
    AAAGAAATAA GATTTCAACC TAATGATCAC AAAACCATCA AAGAAACAGC TGATGAACTG
    AAGATTTTTG AAGGCATCAA ACACCCTAAT CTTGTTCGGT ATTTTGGTGT GGAGCTCCAT
    AGGGAAGAGA TGTACATCTT CATGGAGTAC TGTGATGAGG GCACGCTGGA GGAGGTGTCA
    AAACTGGGCC TTCAGGAGCA TGTCATTAGG CTCTACACAA AGCAGATCAC CATTGCTATC
    AATGTACTAC ATGAACACGG TATTGTTCAT CGAGACATTA AAGGAGCCAA CATCTTTCTT
    ACCTCATCCG GACTGATTAA ACTGGGTGAC TTTGGCTGCT CAGTAAAACT AAAGAACAAC
    ACTCAAACAA TGCCTGGAGA AGTGAACAGT ACATTGGGTA CTGCAGCGTA CATGGCTCCA
    GAAGTAATTA CTAGAGCAAA AGGAGAAGGG CATGGACGTG CAGCAGACAT CTGGAGCCTG
    GGCTGTGTTG TTATAGAGAT GGTCACTGGC AAGAGGCCTT GGCACGAATA TGAGCACAAC
    TTCCAGATCA TGTATAGAGT GGGGATGGGT CATAAACCTC CTATTCCTGA CAAAGTGAGT
    CCGGAGGGGA AAGACTTCCT TTGCCACTGC CTGGAAAGTG ACCCAAAGAT GAGGTGGACA
    GCCAGCCAGC TCCTCGACCA TCCTTTTGTC AAGGTTTGCA CAGATGAAGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139808.1
    CDS169..4782
    Translation

    Target ORF information:

    RefSeq Version XM_015284322.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284322.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGAGACG TCGCCGCACT GCTCCCTGCC GCCGCCGCCA TGGAGGAGCC GCCGCCGCCG 
    CCGCCGGGGA GGAAAGAGTG CAAATCAGAA GATGCTACTT GCAAGATCTC TGAATCTGAA
    CCTGAGGAGT TATCAGATGA AGCAAGTGCT GACACCTTCT ATGGAACTTC TCCTCCTAGT
    ACACCCCGCC AGATGAAGCG TATGTCAACA AAGCATCAGA AAAACAACGT CGGGAGACCT
    GCTAGCCGCT CTACTTTGAA AGAAAAGATG AGTATACCAC CTCAATCCGT GCATAAAGAC
    AATGGGAAAA CCATTGAGAA CGTGGAGGAG CACAGCTATA AGCAAGGGAA AAAAATGCGT
    GACACCCTAA GGACGACAGA ACGAGATCAC AAGAAAAATG TGCAGTGCTC GTTTATGTTA
    GACTCAGTTG GCGGGTCTCT GCCAAAAAAA ACAATTCCAG ATGTTGATCT CAATAAGCCT
    TACCTCAGCC TTGGCTGTAG CAATGCTAAG CTTCCAGTCT CTGTGCCCAT GCCAATCTCC
    AGAACTGCAC GCCAGACTTC TAGGACTGAT TGCCCAGCGG ACCGCTTAAA ATTCTTTGAA
    ACCCTGCGGC TTTTGTTAAA ACTTACCTCA GTCTCGAAAA AAAAGGACAG GGAGCAAAGA
    GGACAGGAAA GCACCTCTTC TGTCTGGCTG AACCGATCCA ATGAACTGAT CTGGCTAGAG
    CTGCAAGCTT GGCATGCTGG CCGGAGTATT AATGACCAAG ACCTTTATCT CTATGCAGCC
    CGTCAAGCTA TCCCTGATAT TATCAATGAA ATCCTTACAT TCAAAGTGAA CTATGGAAGC
    TTCTCCTATG TAAAAAATGG AGCCAGTCTC AATGGTACGT CAGGAGAAGG AGTTTGCAAA
    GCAACACATG GAACCAGTGC TTTGGGTTAC TCAAGTTACC ACGAACACCT CCATCGCCAG
    CGAATCTCAT TTGAGCAAGT AAAGCGAATA ATGGAACTGC TAGAGTATAT AGAAGCACTT
    TATCCATCTC TGCAGGCTCT TCAAAAGGAC TATGAAAAGT ATGCTGCAAA AGACTTCCAG
    GACAGAGTGC AGGCACTCTG TCTGTGGTTG AACATTACAA AAGACTTAAA TCAAAAGCTA
    AGGATTATGG GGACTGTTTT GGGCATCAAA AATCTCTCTG ACATTGGCTG GCCAGTGTTT
    GAAATTCCTT CTCCTCGACC GTCTAAGGAC GATGATCCAG AGGATGAAGG AGTTGATAGG
    GAAACTGAAG TAAAGGAATC CTCAGGAACA TGCACAGAGG AGAGCGATGG CGAAGAACAA
    ATCTCTGAGG AGAATGTTGA GGAACTTAGA CAAGCCATCA AGAACAATTT TACTAATCAG
    CCAAATTTCG ACTTTCAGGA CCATTTTTCA AGGAAGCTTG ATAGGCTTGA ATCTGGGGAT
    GACTCTGCCT CTTGGATTAT TCCAGAATGC AGTGCTGAGG CAGGCTGCAG CAAACACTGT
    TTGACTTCTA TTTACAGGCC GTTTGTAGAC AAAGCACTGA AGCAAATGGG GTTGAGGAAG
    CTAATTTTAA GACTACACAA GCTAATGCAT GGTTCATTGC AAAGGGCTCG TATGGCGTTG
    GTAAGGAGTG ATTGCCCGCT GGAGTTTTCA GAATGTCCAG ATCCCATGTT GTGTTCAGAT
    TACGTGCAGT TATTAAATTC CCCACCTTGC TGTGAGCAAA AATGCAGCAC TGTATCATGG
    GAAGAGCTCA AATCCATGGA TTTGCCATCT TTTGAACCTG CATTCTTGGT CCTTTGCCGA
    GTCCTGCTCA ATGTTATCCA TGAATGTCTC AAGCTAAGGT TGGAACAAAG GCCTGCTGGG
    GAGCCATCTC TTTTGAGTAT TAAACAGCTA GTGAGGGAGT GTAAGGAGGT TCTGAAGGGT
    GGCCTGTTAA TGAAGCAATA TTACCAATTT ATGTTGCGTG AGGTGTTAGA TGACTTGCAA
    AAGATTGATT GCAACATTGA TGCTTTTGAA GAGGACCTGC ATAAAATGTT GATGGTTTAC
    TTTGATTACA TGCGAAGCTG GATCCAAATG CTACAGCAGT TACCTCAAGC ATCTCATAGT
    TTAAAAAATC TCCTAGAGGA AGAATGGAAT TTCACCAAAG AAATAACTCC ATATATAAGA
    GGAGGAGAAG CTCAAGCTGG AAAACTATTT TGTGATATTG CAGGAATGCT GCTGAAATCT
    ACAGGAATAT TTTTAGATTC TGGACTACAA GACAGTTGTG ATGAATTTTG GGCCAGTGCC
    GATGACAGTA CTGCATCAGA TGAAATAAGG AGGTCTGTCA TAGAGACCAG CCGAGCACTC
    AAGGAACTGT TTCATGAAGC TAGAGAAAGG GCCTCCAAGG CTCTTGGATT TGCTAAAATG
    CTGAGGAAGG ACCTTGAAAT AGCAGCAGAG TTTGCATTGT CAGCCCCTGT CCGAGATCTT
    CTGAATGCTC TGAAAACGAA AGAGTATGTG AAGGTACAAA TCCCTGGACT CGAGAATTTA
    CAAGTATTTG TCCCAAATAG TATTGCAGGT GAAAAGTCTG TGATTTTGCA GCTCCTAAAT
    GCGGCTGCTG GGAAAGATTG CTCAAAAGAT TCAGATGAGG TTGCATATGA GGCCTATTTA
    ATAATGACCA AACACAGTGA CAAAGACCAT GAATTAGGTG ACATGTGGAG TGCATGGGAG
    GGCCAACCAG TCAAAGTGGT GCCACAAGTG GAAACTGTTG ATACGCTACG AAGCATGCAG
    GTTGACAACC TCCTTCTCGT AGTCATTCAG TCTGCCCACC TTGTGAGTCA GCGAAAAGCT
    TTCCAACAGT CTATAGAAGG GCTCATATCA CTCCACCAGG AACAGACATC CAGCCAACCA
    GTCATTGCCA AAGCTCTGCA GCAACTAAAG AATGATGCAT TGCAGCTATG CAATAAGATA
    AGCAGTGCCA TTGACAGAGT TGATCATATG TTCACATCTG AGTTTGATGC TGAAGTTGAT
    GAATCTGAAT CTGCGACTTT GCAGCAATAC TACCGGGAAG CCATGATTCA GGCTTACAAT
    TTTGGGTTTG AGTACCACAA AGAAGTTGTT CGTCTGATGT CTGGAGAATT CAGGCAGAAA
    ATTGGAGACA AATATATCAG CTTTGCCAGA AAGTGGATGA ACTACGTGCT AACAAAATGT
    GAGAGTGGCC GAGGCACTCG ACCCAGGTGG GCAACTCAAG GTTTTGATTT TCTTCAAGCC
    ATTGAACCAG CATTTATTTC GGCTCTCCCA GAAGATGATT TCTTGAGTTT GCAAGCTCTG
    ATGAATGAGT GCATTGGCCA CGTTATTGGA AAACCTCACA GTCCTGTTAC AGGTCTGTAC
    CTTGCTATTC ATCGGAACAG TCCCCGTCCC GTGAAAGTAC CTCGATGCCA TAGTGATCCA
    CCGAATCCTC ATCTTATCAT CCCCACTCCT GAAGGCTTCA GGGGTTCCAG TGTCCCCGAA
    AATGATCGAC TGGCTTCAAT AGCAGCCGAG TTGCAGTTCA GATCCCTTAG TCGTCACTCC
    AGTCCCACTG AGGAGCGAGA AGAACCATCA TATCCAAAAG GAGATTCAAG TGGCTCATCC
    CGGCGGAGTT GGGAGCTCAG AACCTTAATC AACCAGACCA AAGACACTGT TTCCAAACAA
    GCACCAATAG AAGCTGTTCA GAAGTCTGTT CAGTTGTTTG AAGAAAGGAG ATACAGAGAG
    ATGAGGAGGA AGAATATCAT TGGCCAAGTT TGCGACACAC CAAAATCCTA CGATAATGTC
    ATGCATGTAG GGTTGAGAAA AGTGACTTTC AAGTGGCAGA GAGGAAACAA AATTGGTGAA
    GGCCAATATG GGAAAGTATA TACCTGCATT AGTGTTGACA CTGGAGAGCT GATGGCTATG
    AAAGAAATAA GATTTCAACC TAATGATCAC AAAACCATCA AAGAAACAGC TGATGAACTG
    AAGATTTTTG AAGGCATCAA ACACCCTAAT CTTGTTCGGT ATTTTGGTGT GGAGCTCCAT
    AGGGAAGAGA TGTACATCTT CATGGAGTAC TGTGATGAGG GCACGCTGGA GGAGGTGTCA
    AAACTGGGCC TTCAGGAGCA TGTCATTAGG CTCTACACAA AGCAGATCAC CATTGCTATC
    AATGTACTAC ATGAACACGG TATTGTTCAT CGAGACATTA AAGGAGCCAA CATCTTTCTT
    ACCTCATCCG GACTGATTAA ACTGGGTGAC TTTGGCTGCT CAGTAAAACT AAAGAACAAC
    ACTCAAACAA TGCCTGGAGA AGTGAACAGT ACATTGGGTA CTGCAGCGTA CATGGCTCCA
    GAAGTAATTA CTAGAGCAAA AGGAGAAGGG CATGGACGTG CAGCAGACAT CTGGAGCCTG
    GGCTGTGTTG TTATAGAGAT GGTCACTGGC AAGAGGCCTT GGCACGAATA TGAGCACAAC
    TTCCAGATCA TGTATAGAGT GGGGATGGGT CATAAACCTC CTATTCCTGA CAAAGTGAGT
    CCGGAGGGGA AAGACTTCCT TTGCCACTGC CTGGAAAGTG ACCCAAAGAT GAGGTGGACA
    GCCAGCCAGC TCCTCGACCA TCCTTTTGTC AAGGTTTGCA CAGATGAAGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33779
    Clone ID Related Accession (Same CDS sequence) XM_015284323.1
    Accession Version XM_015284323.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4602bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product mitogen-activated protein kinase kinase kinase 4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGAGACG TCGCCGCACT GCTCCCTGCC GCCGCCGCCA TGGAGGAGCC GCCGCCGCCG 
    CCGCCGGGGA GGAAAGAGTG CAAATCAGAA GATGCTACTT GCAAGATCTC TGAATCTGAA
    CCTGAGGAGT TATCAGATGA AGCAAGTGCT GACACCTTCT ATGGAACTTC TCCTCCTAGT
    ACACCCCGCC AGATGAAGCG TATGTCAACA AAGCATCAGA AAAACAACGT CGGGAGACCT
    GCTAGCCGCT CTACTTTGAA AGAAAAGATG AGTATACCAC CTCAATCCGT GCATAAAGAC
    AATGGGAAAA CCATTGAGAA CGTGGAGGAG CACAGCTATA AGCAAGGGAA AAAAATGCGT
    GACACCCTAA GGACGACAGA ACGAGATCAC AAGAAAAATG TGCAGTGCTC GTTTATGTTA
    GACTCAGTTG GCGGGTCTCT GCCAAAAAAA ACAATTCCAG ATGTTGATCT CAATAAGCCT
    TACCTCAGCC TTGGCTGTAG CAATGCTAAG CTTCCAGTCT CTGTGCCCAT GCCAATCTCC
    AGAACTGCAC GCCAGACTTC TAGGACTGAT TGCCCAGCGG ACCGCTTAAA ATTCTTTGAA
    ACCCTGCGGC TTTTGTTAAA ACTTACCTCA GTCTCGAAAA AAAAGGACAG GGAGCAAAGA
    GGACAGGAAA GCACCTCTTC TGTCTGGCTG AACCGATCCA ATGAACTGAT CTGGCTAGAG
    CTGCAAGCTT GGCATGCTGG CCGGAGTATT AATGACCAAG ACCTTTATCT CTATGCAGCC
    CGTCAAGCTA TCCCTGATAT TATCAATGAA ATCCTTACAT TCAAAGTGAA CTATGGAAGC
    TTCTCCTATG TAAAAAATGG AGCCAGTCTC AATGGTACGT CAGGAGAAGG AGTTTGCAAA
    GCAACACATG GAACCAGTGC TTTGGGTTAC TCAAGTTACC ACGAACACCT CCATCGCCAG
    CGAATCTCAT TTGAGCAAGT AAAGCGAATA ATGGAACTGC TAGAGTATAT AGAAGCACTT
    TATCCATCTC TGCAGGCTCT TCAAAAGGAC TATGAAAAGT ATGCTGCAAA AGACTTCCAG
    GACAGAGTGC AGGCACTCTG TCTGTGGTTG AACATTACAA AAGACTTAAA TCAAAAGCTA
    AGGATTATGG GGACTGTTTT GGGCATCAAA AATCTCTCTG ACATTGGCTG GCCAGTGTTT
    GAAATTCCTT CTCCTCGACC GTCTAAGGAC GATGATCCAG AGGATGAAGG AGTTGATAGG
    GAAACTGAAG TAAAGGAATC CTCAGGAACA TGCACAGAGG AGAGCGATGG CGAAGAACAA
    ATCTCTGAGG AGAATGTTGA GGAACTTAGA CAAGCCATCA AGAACAATTT TACTAATCAG
    CCAAATTTCG ACTTTCAGGA CCATTTTTCA AGGAAGCTTG ATAGGCTTGA ATCTGGGGAT
    GACTCTGCCT CTTGGATTAT TCCAGAATGC AGTGCTGAGG CAGGCTGCAG CAAACACTGT
    TTGACTTCTA TTTACAGGCC GTTTGTAGAC AAAGCACTGA AGCAAATGGG GTTGAGGAAG
    CTAATTTTAA GACTACACAA GCTAATGCAT GGTTCATTGC AAAGGGCTCG TATGGCGTTG
    GTAAGGAGTG ATTGCCCGCT GGAGTTTTCA GAATGTCCAG ATCCCATGTT GTGTTCAGAT
    TACGTGCAGT TATTAAATTC CCCACCTTGC TGTGAGCAAA AATGCAGCAC TGTATCATGG
    GAAGAGCTCA AATCCATGGA TTTGCCATCT TTTGAACCTG CATTCTTGGT CCTTTGCCGA
    GTCCTGCTCA ATGTTATCCA TGAATGTCTC AAGCTAAGGT TGGAACAAAG GCCTGCTGGG
    GAGCCATCTC TTTTGAGTAT TAAACAGCTA GTGAGGGAGT GTAAGGAGGT TCTGAAGGGT
    GGCCTGTTAA TGAAGCAATA TTACCAATTT ATGTTGCGTG AGGTGTTAGA TGACTTGCAA
    AAGATTGATT GCAACATTGA TGCTTTTGAA GAGGACCTGC ATAAAATGTT GATGGTTTAC
    TTTGATTACA TGCGAAGCTG GATCCAAATG CTACAGCAGT TACCTCAAGC ATCTCATAGT
    TTAAAAAATC TCCTAGAGGA AGAATGGAAT TTCACCAAAG AAATAACTCC ATATATAAGA
    GGAGGAGAAG CTCAAGCTGG AAAACTATTT TGTGATATTG CAGGAATGCT GCTGAAATCT
    ACAGGAATAT TTTTAGATTC TGGACTACAA GACAGTTGTG ATGAATTTTG GGCCAGTGCC
    GATGACAGTA CTGCATCAGA TGAAATAAGG AGGTCTGTCA TAGAGACCAG CCGAGCACTC
    AAGGAACTGT TTCATGAAGC TAGAGAAAGG GCCTCCAAGG CTCTTGGATT TGCTAAAATG
    CTGAGGAAGG ACCTTGAAAT AGCAGCAGAG TTTGCATTGT CAGCCCCTGT CCGAGATCTT
    CTGAATGCTC TGAAAACGAA AGAGTATGTG AAGGTACAAA TCCCTGGACT CGAGAATTTA
    CAAGTATTTG TCCCAAATAG TATTGCAGGT GAAAAGTCTG TGATTTTGCA GCTCCTAAAT
    GCGGCTGCTG GGAAAGATTG CTCAAAAGAT TCAGATGAGG TTGCATATGA GGCCTATTTA
    ATAATGACCA AACACAGTGA CAAAGACCAT GAATTAGGTG ACATGTGGAG TGCATGGGAG
    GGCCAACCAG TCAAAGTGGT GCCACAAGTG GAAACTGTTG ATACGCTACG AAGCATGCAG
    GTTGACAACC TCCTTCTCGT AGTCATTCAG TCTGCCCACC TTGTGAGTCA GCGAAAAGCT
    TTCCAACAGT CTATAGAAGG GCTCATATCA CTCCACCAGG AACAGACATC CAGCCAACCA
    GTCATTGCCA AAGCTCTGCA GCAACTAAAG AATGATGCAT TGCAGCTATG CAATAAGATA
    AGCAGTGCCA TTGACAGAGT TGATCATATG TTCACATCTG AGTTTGATGC TGAAGTTGAT
    GAATCTGAAT CTGCGACTTT GCAGCAATAC TACCGGGAAG CCATGATTCA GGCTTACAAT
    TTTGGGTTTG AGTACCACAA AGAAGTTGTT CGTCTGATGT CTGGAGAATT CAGGCAGAAA
    ATTGGAGACA AATATATCAG CTTTGCCAGA AAGTGGATGA ACTACGTGCT AACAAAATGT
    GAGAGTGGCC GAGGCACTCG ACCCAGGTGG GCAACTCAAG GTTTTGATTT TCTTCAAGCC
    ATTGAACCAG CATTTATTTC GGCTCTCCCA GAAGATGATT TCTTGAGTTT GCAAGCTCTG
    ATGAATGAGT GCATTGGCCA CGTTATTGGA AAACCTCACA GTCCTGTTAC AGCTATTCAT
    CGGAACAGTC CCCGTCCCGT GAAAGTACCT CGATGCCATA GTGATCCACC GAATCCTCAT
    CTTATCATCC CCACTCCTGA AGGCTTCAGG GGTTCCAGTG TCCCCGAAAA TGATCGACTG
    GCTTCAATAG CAGCCGAGTT GCAGTTCAGA TCCCTTAGTC GTCACTCCAG TCCCACTGAG
    GAGCGAGAAG AACCATCATA TCCAAAAGGA GATTCAAGTG GCTCATCCCG GCGGAGTTGG
    GAGCTCAGAA CCTTAATCAA CCAGACCAAA GACACTGTTT CCAAACAAGC ACCAATAGAA
    GCTGTTCAGA AGTCTGTTCA GTTGTTTGAA GAAAGGAGAT ACAGAGAGAT GAGGAGGAAG
    AATATCATTG GCCAAGTTTG CGACACACCA AAATCCTACG ATAATGTCAT GCATGTAGGG
    TTGAGAAAAG TGACTTTCAA GTGGCAGAGA GGAAACAAAA TTGGTGAAGG CCAATATGGG
    AAAGTATATA CCTGCATTAG TGTTGACACT GGAGAGCTGA TGGCTATGAA AGAAATAAGA
    TTTCAACCTA ATGATCACAA AACCATCAAA GAAACAGCTG ATGAACTGAA GATTTTTGAA
    GGCATCAAAC ACCCTAATCT TGTTCGGTAT TTTGGTGTGG AGCTCCATAG GGAAGAGATG
    TACATCTTCA TGGAGTACTG TGATGAGGGC ACGCTGGAGG AGGTGTCAAA ACTGGGCCTT
    CAGGAGCATG TCATTAGGCT CTACACAAAG CAGATCACCA TTGCTATCAA TGTACTACAT
    GAACACGGTA TTGTTCATCG AGACATTAAA GGAGCCAACA TCTTTCTTAC CTCATCCGGA
    CTGATTAAAC TGGGTGACTT TGGCTGCTCA GTAAAACTAA AGAACAACAC TCAAACAATG
    CCTGGAGAAG TGAACAGTAC ATTGGGTACT GCAGCGTACA TGGCTCCAGA AGTAATTACT
    AGAGCAAAAG GAGAAGGGCA TGGACGTGCA GCAGACATCT GGAGCCTGGG CTGTGTTGTT
    ATAGAGATGG TCACTGGCAA GAGGCCTTGG CACGAATATG AGCACAACTT CCAGATCATG
    TATAGAGTGG GGATGGGTCA TAAACCTCCT ATTCCTGACA AAGTGAGTCC GGAGGGGAAA
    GACTTCCTTT GCCACTGCCT GGAAAGTGAC CCAAAGATGA GGTGGACAGC CAGCCAGCTC
    CTCGACCATC CTTTTGTCAA GGTTTGCACA GATGAAGAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139809.1
    CDS169..4770
    Translation

    Target ORF information:

    RefSeq Version XM_015284323.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284323.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGAGACG TCGCCGCACT GCTCCCTGCC GCCGCCGCCA TGGAGGAGCC GCCGCCGCCG 
    CCGCCGGGGA GGAAAGAGTG CAAATCAGAA GATGCTACTT GCAAGATCTC TGAATCTGAA
    CCTGAGGAGT TATCAGATGA AGCAAGTGCT GACACCTTCT ATGGAACTTC TCCTCCTAGT
    ACACCCCGCC AGATGAAGCG TATGTCAACA AAGCATCAGA AAAACAACGT CGGGAGACCT
    GCTAGCCGCT CTACTTTGAA AGAAAAGATG AGTATACCAC CTCAATCCGT GCATAAAGAC
    AATGGGAAAA CCATTGAGAA CGTGGAGGAG CACAGCTATA AGCAAGGGAA AAAAATGCGT
    GACACCCTAA GGACGACAGA ACGAGATCAC AAGAAAAATG TGCAGTGCTC GTTTATGTTA
    GACTCAGTTG GCGGGTCTCT GCCAAAAAAA ACAATTCCAG ATGTTGATCT CAATAAGCCT
    TACCTCAGCC TTGGCTGTAG CAATGCTAAG CTTCCAGTCT CTGTGCCCAT GCCAATCTCC
    AGAACTGCAC GCCAGACTTC TAGGACTGAT TGCCCAGCGG ACCGCTTAAA ATTCTTTGAA
    ACCCTGCGGC TTTTGTTAAA ACTTACCTCA GTCTCGAAAA AAAAGGACAG GGAGCAAAGA
    GGACAGGAAA GCACCTCTTC TGTCTGGCTG AACCGATCCA ATGAACTGAT CTGGCTAGAG
    CTGCAAGCTT GGCATGCTGG CCGGAGTATT AATGACCAAG ACCTTTATCT CTATGCAGCC
    CGTCAAGCTA TCCCTGATAT TATCAATGAA ATCCTTACAT TCAAAGTGAA CTATGGAAGC
    TTCTCCTATG TAAAAAATGG AGCCAGTCTC AATGGTACGT CAGGAGAAGG AGTTTGCAAA
    GCAACACATG GAACCAGTGC TTTGGGTTAC TCAAGTTACC ACGAACACCT CCATCGCCAG
    CGAATCTCAT TTGAGCAAGT AAAGCGAATA ATGGAACTGC TAGAGTATAT AGAAGCACTT
    TATCCATCTC TGCAGGCTCT TCAAAAGGAC TATGAAAAGT ATGCTGCAAA AGACTTCCAG
    GACAGAGTGC AGGCACTCTG TCTGTGGTTG AACATTACAA AAGACTTAAA TCAAAAGCTA
    AGGATTATGG GGACTGTTTT GGGCATCAAA AATCTCTCTG ACATTGGCTG GCCAGTGTTT
    GAAATTCCTT CTCCTCGACC GTCTAAGGAC GATGATCCAG AGGATGAAGG AGTTGATAGG
    GAAACTGAAG TAAAGGAATC CTCAGGAACA TGCACAGAGG AGAGCGATGG CGAAGAACAA
    ATCTCTGAGG AGAATGTTGA GGAACTTAGA CAAGCCATCA AGAACAATTT TACTAATCAG
    CCAAATTTCG ACTTTCAGGA CCATTTTTCA AGGAAGCTTG ATAGGCTTGA ATCTGGGGAT
    GACTCTGCCT CTTGGATTAT TCCAGAATGC AGTGCTGAGG CAGGCTGCAG CAAACACTGT
    TTGACTTCTA TTTACAGGCC GTTTGTAGAC AAAGCACTGA AGCAAATGGG GTTGAGGAAG
    CTAATTTTAA GACTACACAA GCTAATGCAT GGTTCATTGC AAAGGGCTCG TATGGCGTTG
    GTAAGGAGTG ATTGCCCGCT GGAGTTTTCA GAATGTCCAG ATCCCATGTT GTGTTCAGAT
    TACGTGCAGT TATTAAATTC CCCACCTTGC TGTGAGCAAA AATGCAGCAC TGTATCATGG
    GAAGAGCTCA AATCCATGGA TTTGCCATCT TTTGAACCTG CATTCTTGGT CCTTTGCCGA
    GTCCTGCTCA ATGTTATCCA TGAATGTCTC AAGCTAAGGT TGGAACAAAG GCCTGCTGGG
    GAGCCATCTC TTTTGAGTAT TAAACAGCTA GTGAGGGAGT GTAAGGAGGT TCTGAAGGGT
    GGCCTGTTAA TGAAGCAATA TTACCAATTT ATGTTGCGTG AGGTGTTAGA TGACTTGCAA
    AAGATTGATT GCAACATTGA TGCTTTTGAA GAGGACCTGC ATAAAATGTT GATGGTTTAC
    TTTGATTACA TGCGAAGCTG GATCCAAATG CTACAGCAGT TACCTCAAGC ATCTCATAGT
    TTAAAAAATC TCCTAGAGGA AGAATGGAAT TTCACCAAAG AAATAACTCC ATATATAAGA
    GGAGGAGAAG CTCAAGCTGG AAAACTATTT TGTGATATTG CAGGAATGCT GCTGAAATCT
    ACAGGAATAT TTTTAGATTC TGGACTACAA GACAGTTGTG ATGAATTTTG GGCCAGTGCC
    GATGACAGTA CTGCATCAGA TGAAATAAGG AGGTCTGTCA TAGAGACCAG CCGAGCACTC
    AAGGAACTGT TTCATGAAGC TAGAGAAAGG GCCTCCAAGG CTCTTGGATT TGCTAAAATG
    CTGAGGAAGG ACCTTGAAAT AGCAGCAGAG TTTGCATTGT CAGCCCCTGT CCGAGATCTT
    CTGAATGCTC TGAAAACGAA AGAGTATGTG AAGGTACAAA TCCCTGGACT CGAGAATTTA
    CAAGTATTTG TCCCAAATAG TATTGCAGGT GAAAAGTCTG TGATTTTGCA GCTCCTAAAT
    GCGGCTGCTG GGAAAGATTG CTCAAAAGAT TCAGATGAGG TTGCATATGA GGCCTATTTA
    ATAATGACCA AACACAGTGA CAAAGACCAT GAATTAGGTG ACATGTGGAG TGCATGGGAG
    GGCCAACCAG TCAAAGTGGT GCCACAAGTG GAAACTGTTG ATACGCTACG AAGCATGCAG
    GTTGACAACC TCCTTCTCGT AGTCATTCAG TCTGCCCACC TTGTGAGTCA GCGAAAAGCT
    TTCCAACAGT CTATAGAAGG GCTCATATCA CTCCACCAGG AACAGACATC CAGCCAACCA
    GTCATTGCCA AAGCTCTGCA GCAACTAAAG AATGATGCAT TGCAGCTATG CAATAAGATA
    AGCAGTGCCA TTGACAGAGT TGATCATATG TTCACATCTG AGTTTGATGC TGAAGTTGAT
    GAATCTGAAT CTGCGACTTT GCAGCAATAC TACCGGGAAG CCATGATTCA GGCTTACAAT
    TTTGGGTTTG AGTACCACAA AGAAGTTGTT CGTCTGATGT CTGGAGAATT CAGGCAGAAA
    ATTGGAGACA AATATATCAG CTTTGCCAGA AAGTGGATGA ACTACGTGCT AACAAAATGT
    GAGAGTGGCC GAGGCACTCG ACCCAGGTGG GCAACTCAAG GTTTTGATTT TCTTCAAGCC
    ATTGAACCAG CATTTATTTC GGCTCTCCCA GAAGATGATT TCTTGAGTTT GCAAGCTCTG
    ATGAATGAGT GCATTGGCCA CGTTATTGGA AAACCTCACA GTCCTGTTAC AGCTATTCAT
    CGGAACAGTC CCCGTCCCGT GAAAGTACCT CGATGCCATA GTGATCCACC GAATCCTCAT
    CTTATCATCC CCACTCCTGA AGGCTTCAGG GGTTCCAGTG TCCCCGAAAA TGATCGACTG
    GCTTCAATAG CAGCCGAGTT GCAGTTCAGA TCCCTTAGTC GTCACTCCAG TCCCACTGAG
    GAGCGAGAAG AACCATCATA TCCAAAAGGA GATTCAAGTG GCTCATCCCG GCGGAGTTGG
    GAGCTCAGAA CCTTAATCAA CCAGACCAAA GACACTGTTT CCAAACAAGC ACCAATAGAA
    GCTGTTCAGA AGTCTGTTCA GTTGTTTGAA GAAAGGAGAT ACAGAGAGAT GAGGAGGAAG
    AATATCATTG GCCAAGTTTG CGACACACCA AAATCCTACG ATAATGTCAT GCATGTAGGG
    TTGAGAAAAG TGACTTTCAA GTGGCAGAGA GGAAACAAAA TTGGTGAAGG CCAATATGGG
    AAAGTATATA CCTGCATTAG TGTTGACACT GGAGAGCTGA TGGCTATGAA AGAAATAAGA
    TTTCAACCTA ATGATCACAA AACCATCAAA GAAACAGCTG ATGAACTGAA GATTTTTGAA
    GGCATCAAAC ACCCTAATCT TGTTCGGTAT TTTGGTGTGG AGCTCCATAG GGAAGAGATG
    TACATCTTCA TGGAGTACTG TGATGAGGGC ACGCTGGAGG AGGTGTCAAA ACTGGGCCTT
    CAGGAGCATG TCATTAGGCT CTACACAAAG CAGATCACCA TTGCTATCAA TGTACTACAT
    GAACACGGTA TTGTTCATCG AGACATTAAA GGAGCCAACA TCTTTCTTAC CTCATCCGGA
    CTGATTAAAC TGGGTGACTT TGGCTGCTCA GTAAAACTAA AGAACAACAC TCAAACAATG
    CCTGGAGAAG TGAACAGTAC ATTGGGTACT GCAGCGTACA TGGCTCCAGA AGTAATTACT
    AGAGCAAAAG GAGAAGGGCA TGGACGTGCA GCAGACATCT GGAGCCTGGG CTGTGTTGTT
    ATAGAGATGG TCACTGGCAA GAGGCCTTGG CACGAATATG AGCACAACTT CCAGATCATG
    TATAGAGTGG GGATGGGTCA TAAACCTCCT ATTCCTGACA AAGTGAGTCC GGAGGGGAAA
    GACTTCCTTT GCCACTGCCT GGAAAGTGAC CCAAAGATGA GGTGGACAGC CAGCCAGCTC
    CTCGACCATC CTTTTGTCAA GGTTTGCACA GATGAAGAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33781
    Clone ID Related Accession (Same CDS sequence) XM_015284326.1
    Accession Version XM_015284326.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4488bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product mitogen-activated protein kinase kinase kinase 4 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGAGACG TCGCCGCACT GCTCCCTGCC GCCGCCGCCA TGGAGGAGCC GCCGCCGCCG 
    CCGCCGGGGA GGAAAGAGTG CAAATCAGAA GATGCTACTT GCAAGATCTC TGAATCTGAA
    CCTGAGGAGT TATCAGATGA AGCAAGTGCT GACACCTTCT ATGGAACTTC TCCTCCTAGT
    ACACCCCGCC AGATGAAGCG TATGTCAACA AAGCATCAGA AAAACAACGT CGGGAGACCT
    GCTAGCCGCT CTACTTTGAA AGAAAAGATG AGTATACCAC CTCAATCCGT GCATAAAGAC
    AATGGGAAAA CCATTGAGAA CGTGGAGGAG CACAGCTATA AGCAAGGGAA AAAAATGCGT
    GACACCCTAA GGACGACAGA ACGAGATCAC AAGAAAAATG TGCAGTGCTC GTTTATGTTA
    GACTCAGTTG GCGGGTCTCT GCCAAAAAAA ACAATTCCAG ATGTTGATCT CAATAAGCCT
    TACCTCAGCC TTGGCTGTAG CAATGCTAAG CTTCCAGTCT CTGTGCCCAT GCCAATCTCC
    AGAACTGCAC GCCAGACTTC TAGGACTGAT TGCCCAGCGG ACCGCTTAAA ATTCTTTGAA
    ACCCTGCGGC TTTTGTTAAA ACTTACCTCA GTCTCGAAAA AAAAGGACAG GGAGCAAAGA
    GGACAGGAAA GCACCTCTTC TGTCTGGCTG AACCGATCCA ATGAACTGAT CTGGCTAGAG
    CTGCAAGCTT GGCATGCTGG CCGGAGTATT AATGACCAAG ACCTTTATCT CTATGCAGCC
    CGTCAAGCTA TCCCTGATAT TATCAATGAA ATCCTTACAT TCAAAGTGAA CTATGGAAGC
    TTCTCCTATG TAAAAAATGG AGCCAGTCTC AATGGTACGT CAGGAGAAGG AGTTTGCAAA
    GCAACACATG GAACCAGTGC TTTGGGTTAC TCAAGTTACC ACGAACACCT CCATCGCCAG
    CGAATCTCAT TTGAGCAAGT AAAGCGAATA ATGGAACTGC TAGAGTATAT AGAAGCACTT
    TATCCATCTC TGCAGGCTCT TCAAAAGGAC TATGAAAAGT ATGCTGCAAA AGACTTCCAG
    GACAGAGTGC AGGCACTCTG TCTGTGGTTG AACATTACAA AAGACTTAAA TCAAAAGCTA
    AGGATTATGG GGACTGTTTT GGGCATCAAA AATCTCTCTG ACATTGGCTG GCCAGTGTTT
    GAAATTCCTT CTCCTCGACC GTCTAAGGAC GATGATCCAG AGGATGAAGG AGTTGATAGG
    GAAACTGAAG TAAAGGAATC CTCAGGAACA TGCACAGAGG AGAGCGATGG CGAAGAACAA
    ATCTCTGAGG AGAATGTTGA GGAACTTAGA CAAGCCATCA AGAACAATTT TACTAATCAG
    CCAAATTTCG ACTTTCAGGA CCATTTTTCA AGGAAGCTTG ATAGGCTTGA ATCTGGGGAT
    GACTCTGCCT CTTGGATTAT TCCAGAATGC AGTGCTGAGG CAGGCTGCAG CAAACACTGT
    TTGACTTCTA TTTACAGGCC GTTTGTAGAC AAAGCACTGA AGCAAATGGG GTTGAGGAAG
    CTAATTTTAA GACTACACAA GCTAATGCAT GGTTCATTGC AAAGGGCTCG TATGGCGTTG
    GTAAGGAGTG ATTGCCCGCT GGAGCTAGTG AGGGAGTGTA AGGAGGTTCT GAAGGGTGGC
    CTGTTAATGA AGCAATATTA CCAATTTATG TTGCGTGAGG TGTTAGATGA CTTGCAAAAG
    ATTGATTGCA ACATTGATGC TTTTGAAGAG GACCTGCATA AAATGTTGAT GGTTTACTTT
    GATTACATGC GAAGCTGGAT CCAAATGCTA CAGCAGTTAC CTCAAGCATC TCATAGTTTA
    AAAAATCTCC TAGAGGAAGA ATGGAATTTC ACCAAAGAAA TAACTCCATA TATAAGAGGA
    GGAGAAGCTC AAGCTGGAAA ACTATTTTGT GATATTGCAG GAATGCTGCT GAAATCTACA
    GGAATATTTT TAGATTCTGG ACTACAAGAC AGTTGTGATG AATTTTGGGC CAGTGCCGAT
    GACAGTACTG CATCAGATGA AATAAGGAGG TCTGTCATAG AGACCAGCCG AGCACTCAAG
    GAACTGTTTC ATGAAGCTAG AGAAAGGGCC TCCAAGGCTC TTGGATTTGC TAAAATGCTG
    AGGAAGGACC TTGAAATAGC AGCAGAGTTT GCATTGTCAG CCCCTGTCCG AGATCTTCTG
    AATGCTCTGA AAACGAAAGA GTATGTGAAG GTACAAATCC CTGGACTCGA GAATTTACAA
    GTATTTGTCC CAAATAGTAT TGCAGGTGAA AAGTCTGTGA TTTTGCAGCT CCTAAATGCG
    GCTGCTGGGA AAGATTGCTC AAAAGATTCA GATGAGGTTG CATATGAGGC CTATTTAATA
    ATGACCAAAC ACAGTGACAA AGACCATGAA TTAGGTGACA TGTGGAGTGC ATGGGAGGGC
    CAACCAGTCA AAGTGGTGCC ACAAGTGGAA ACTGTTGATA CGCTACGAAG CATGCAGGTT
    GACAACCTCC TTCTCGTAGT CATTCAGTCT GCCCACCTTG TGAGTCAGCG AAAAGCTTTC
    CAACAGTCTA TAGAAGGGCT CATATCACTC CACCAGGAAC AGACATCCAG CCAACCAGTC
    ATTGCCAAAG CTCTGCAGCA ACTAAAGAAT GATGCATTGC AGCTATGCAA TAAGATAAGC
    AGTGCCATTG ACAGAGTTGA TCATATGTTC ACATCTGAGT TTGATGCTGA AGTTGATGAA
    TCTGAATCTG CGACTTTGCA GCAATACTAC CGGGAAGCCA TGATTCAGGC TTACAATTTT
    GGGTTTGAGT ACCACAAAGA AGTTGTTCGT CTGATGTCTG GAGAATTCAG GCAGAAAATT
    GGAGACAAAT ATATCAGCTT TGCCAGAAAG TGGATGAACT ACGTGCTAAC AAAATGTGAG
    AGTGGCCGAG GCACTCGACC CAGGTGGGCA ACTCAAGGTT TTGATTTTCT TCAAGCCATT
    GAACCAGCAT TTATTTCGGC TCTCCCAGAA GATGATTTCT TGAGTTTGCA AGCTCTGATG
    AATGAGTGCA TTGGCCACGT TATTGGAAAA CCTCACAGTC CTGTTACAGG TCTGTACCTT
    GCTATTCATC GGAACAGTCC CCGTCCCGTG AAAGTACCTC GATGCCATAG TGATCCACCG
    AATCCTCATC TTATCATCCC CACTCCTGAA GGCTTCAGCA CACGGAGCGT CCCTTGCGAT
    GCACGGAACC CTTGCAACGT TACTGTCAGT CGCCCCGCTC CCATTGGTAG TGACTCAACT
    CAACCTAAAT CTGTCAGCAG TGCAAATGAT ACCAGGGGTT CCAGTGTCCC CGAAAATGAT
    CGACTGGCTT CAATAGCAGC CGAGTTGCAG TTCAGATCCC TTAGTCGTCA CTCCAGTCCC
    ACTGAGGAGC GAGAAGAACC ATCATATCCA AAAGGAGATT CAAGTGGCTC ATCCCGGCGG
    AGTTGGGAGC TCAGAACCTT AATCAACCAG ACCAAAGACA CTGTTTCCAA ACAAGCACCA
    ATAGAAGCTG TTCAGAAGTC TGTTCAGTTG TTTGAAGAAA GGAGATACAG AGAGATGAGG
    AGGAAGAATA TCATTGGCCA AGTTTGCGAC ACACCAAAAT CCTACGATAA TGTCATGCAT
    GTAGGGTTGA GAAAAGTGAC TTTCAAGTGG CAGAGAGGAA ACAAAATTGG TGAAGGCCAA
    TATGGGAAAG TATATACCTG CATTAGTGTT GACACTGGAG AGCTGATGGC TATGAAAGAA
    ATAAGATTTC AACCTAATGA TCACAAAACC ATCAAAGAAA CAGCTGATGA ACTGAAGATT
    TTTGAAGGCA TCAAACACCC TAATCTTGTT CGGTATTTTG GTGTGGAGCT CCATAGGGAA
    GAGATGTACA TCTTCATGGA GTACTGTGAT GAGGGCACGC TGGAGGAGGT GTCAAAACTG
    GGCCTTCAGG AGCATGTCAT TAGGCTCTAC ACAAAGCAGA TCACCATTGC TATCAATGTA
    CTACATGAAC ACGGTATTGT TCATCGAGAC ATTAAAGGAG CCAACATCTT TCTTACCTCA
    TCCGGACTGA TTAAACTGGG TGACTTTGGC TGCTCAGTAA AACTAAAGAA CAACACTCAA
    ACAATGCCTG GAGAAGTGAA CAGTACATTG GGTACTGCAG CGTACATGGC TCCAGAAGTA
    ATTACTAGAG CAAAAGGAGA AGGGCATGGA CGTGCAGCAG ACATCTGGAG CCTGGGCTGT
    GTTGTTATAG AGATGGTCAC TGGCAAGAGG CCTTGGCACG AATATGAGCA CAACTTCCAG
    ATCATGTATA GAGTGGGGAT GGGTCATAAA CCTCCTATTC CTGACAAAGT GAGTCCGGAG
    GGGAAAGACT TCCTTTGCCA CTGCCTGGAA AGTGACCCAA AGATGAGGTG GACAGCCAGC
    CAGCTCCTCG ACCATCCTTT TGTCAAGGTT TGCACAGATG AAGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015139812.1
    CDS169..4656
    Translation

    Target ORF information:

    RefSeq Version XM_015284326.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015284326.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGAGACG TCGCCGCACT GCTCCCTGCC GCCGCCGCCA TGGAGGAGCC GCCGCCGCCG 
    CCGCCGGGGA GGAAAGAGTG CAAATCAGAA GATGCTACTT GCAAGATCTC TGAATCTGAA
    CCTGAGGAGT TATCAGATGA AGCAAGTGCT GACACCTTCT ATGGAACTTC TCCTCCTAGT
    ACACCCCGCC AGATGAAGCG TATGTCAACA AAGCATCAGA AAAACAACGT CGGGAGACCT
    GCTAGCCGCT CTACTTTGAA AGAAAAGATG AGTATACCAC CTCAATCCGT GCATAAAGAC
    AATGGGAAAA CCATTGAGAA CGTGGAGGAG CACAGCTATA AGCAAGGGAA AAAAATGCGT
    GACACCCTAA GGACGACAGA ACGAGATCAC AAGAAAAATG TGCAGTGCTC GTTTATGTTA
    GACTCAGTTG GCGGGTCTCT GCCAAAAAAA ACAATTCCAG ATGTTGATCT CAATAAGCCT
    TACCTCAGCC TTGGCTGTAG CAATGCTAAG CTTCCAGTCT CTGTGCCCAT GCCAATCTCC
    AGAACTGCAC GCCAGACTTC TAGGACTGAT TGCCCAGCGG ACCGCTTAAA ATTCTTTGAA
    ACCCTGCGGC TTTTGTTAAA ACTTACCTCA GTCTCGAAAA AAAAGGACAG GGAGCAAAGA
    GGACAGGAAA GCACCTCTTC TGTCTGGCTG AACCGATCCA ATGAACTGAT CTGGCTAGAG
    CTGCAAGCTT GGCATGCTGG CCGGAGTATT AATGACCAAG ACCTTTATCT CTATGCAGCC
    CGTCAAGCTA TCCCTGATAT TATCAATGAA ATCCTTACAT TCAAAGTGAA CTATGGAAGC
    TTCTCCTATG TAAAAAATGG AGCCAGTCTC AATGGTACGT CAGGAGAAGG AGTTTGCAAA
    GCAACACATG GAACCAGTGC TTTGGGTTAC TCAAGTTACC ACGAACACCT CCATCGCCAG
    CGAATCTCAT TTGAGCAAGT AAAGCGAATA ATGGAACTGC TAGAGTATAT AGAAGCACTT
    TATCCATCTC TGCAGGCTCT TCAAAAGGAC TATGAAAAGT ATGCTGCAAA AGACTTCCAG
    GACAGAGTGC AGGCACTCTG TCTGTGGTTG AACATTACAA AAGACTTAAA TCAAAAGCTA
    AGGATTATGG GGACTGTTTT GGGCATCAAA AATCTCTCTG ACATTGGCTG GCCAGTGTTT
    GAAATTCCTT CTCCTCGACC GTCTAAGGAC GATGATCCAG AGGATGAAGG AGTTGATAGG
    GAAACTGAAG TAAAGGAATC CTCAGGAACA TGCACAGAGG AGAGCGATGG CGAAGAACAA
    ATCTCTGAGG AGAATGTTGA GGAACTTAGA CAAGCCATCA AGAACAATTT TACTAATCAG
    CCAAATTTCG ACTTTCAGGA CCATTTTTCA AGGAAGCTTG ATAGGCTTGA ATCTGGGGAT
    GACTCTGCCT CTTGGATTAT TCCAGAATGC AGTGCTGAGG CAGGCTGCAG CAAACACTGT
    TTGACTTCTA TTTACAGGCC GTTTGTAGAC AAAGCACTGA AGCAAATGGG GTTGAGGAAG
    CTAATTTTAA GACTACACAA GCTAATGCAT GGTTCATTGC AAAGGGCTCG TATGGCGTTG
    GTAAGGAGTG ATTGCCCGCT GGAGCTAGTG AGGGAGTGTA AGGAGGTTCT GAAGGGTGGC
    CTGTTAATGA AGCAATATTA CCAATTTATG TTGCGTGAGG TGTTAGATGA CTTGCAAAAG
    ATTGATTGCA ACATTGATGC TTTTGAAGAG GACCTGCATA AAATGTTGAT GGTTTACTTT
    GATTACATGC GAAGCTGGAT CCAAATGCTA CAGCAGTTAC CTCAAGCATC TCATAGTTTA
    AAAAATCTCC TAGAGGAAGA ATGGAATTTC ACCAAAGAAA TAACTCCATA TATAAGAGGA
    GGAGAAGCTC AAGCTGGAAA ACTATTTTGT GATATTGCAG GAATGCTGCT GAAATCTACA
    GGAATATTTT TAGATTCTGG ACTACAAGAC AGTTGTGATG AATTTTGGGC CAGTGCCGAT
    GACAGTACTG CATCAGATGA AATAAGGAGG TCTGTCATAG AGACCAGCCG AGCACTCAAG
    GAACTGTTTC ATGAAGCTAG AGAAAGGGCC TCCAAGGCTC TTGGATTTGC TAAAATGCTG
    AGGAAGGACC TTGAAATAGC AGCAGAGTTT GCATTGTCAG CCCCTGTCCG AGATCTTCTG
    AATGCTCTGA AAACGAAAGA GTATGTGAAG GTACAAATCC CTGGACTCGA GAATTTACAA
    GTATTTGTCC CAAATAGTAT TGCAGGTGAA AAGTCTGTGA TTTTGCAGCT CCTAAATGCG
    GCTGCTGGGA AAGATTGCTC AAAAGATTCA GATGAGGTTG CATATGAGGC CTATTTAATA
    ATGACCAAAC ACAGTGACAA AGACCATGAA TTAGGTGACA TGTGGAGTGC ATGGGAGGGC
    CAACCAGTCA AAGTGGTGCC ACAAGTGGAA ACTGTTGATA CGCTACGAAG CATGCAGGTT
    GACAACCTCC TTCTCGTAGT CATTCAGTCT GCCCACCTTG TGAGTCAGCG AAAAGCTTTC
    CAACAGTCTA TAGAAGGGCT CATATCACTC CACCAGGAAC AGACATCCAG CCAACCAGTC
    ATTGCCAAAG CTCTGCAGCA ACTAAAGAAT GATGCATTGC AGCTATGCAA TAAGATAAGC
    AGTGCCATTG ACAGAGTTGA TCATATGTTC ACATCTGAGT TTGATGCTGA AGTTGATGAA
    TCTGAATCTG CGACTTTGCA GCAATACTAC CGGGAAGCCA TGATTCAGGC TTACAATTTT
    GGGTTTGAGT ACCACAAAGA AGTTGTTCGT CTGATGTCTG GAGAATTCAG GCAGAAAATT
    GGAGACAAAT ATATCAGCTT TGCCAGAAAG TGGATGAACT ACGTGCTAAC AAAATGTGAG
    AGTGGCCGAG GCACTCGACC CAGGTGGGCA ACTCAAGGTT TTGATTTTCT TCAAGCCATT
    GAACCAGCAT TTATTTCGGC TCTCCCAGAA GATGATTTCT TGAGTTTGCA AGCTCTGATG
    AATGAGTGCA TTGGCCACGT TATTGGAAAA CCTCACAGTC CTGTTACAGG TCTGTACCTT
    GCTATTCATC GGAACAGTCC CCGTCCCGTG AAAGTACCTC GATGCCATAG TGATCCACCG
    AATCCTCATC TTATCATCCC CACTCCTGAA GGCTTCAGCA CACGGAGCGT CCCTTGCGAT
    GCACGGAACC CTTGCAACGT TACTGTCAGT CGCCCCGCTC CCATTGGTAG TGACTCAACT
    CAACCTAAAT CTGTCAGCAG TGCAAATGAT ACCAGGGGTT CCAGTGTCCC CGAAAATGAT
    CGACTGGCTT CAATAGCAGC CGAGTTGCAG TTCAGATCCC TTAGTCGTCA CTCCAGTCCC
    ACTGAGGAGC GAGAAGAACC ATCATATCCA AAAGGAGATT CAAGTGGCTC ATCCCGGCGG
    AGTTGGGAGC TCAGAACCTT AATCAACCAG ACCAAAGACA CTGTTTCCAA ACAAGCACCA
    ATAGAAGCTG TTCAGAAGTC TGTTCAGTTG TTTGAAGAAA GGAGATACAG AGAGATGAGG
    AGGAAGAATA TCATTGGCCA AGTTTGCGAC ACACCAAAAT CCTACGATAA TGTCATGCAT
    GTAGGGTTGA GAAAAGTGAC TTTCAAGTGG CAGAGAGGAA ACAAAATTGG TGAAGGCCAA
    TATGGGAAAG TATATACCTG CATTAGTGTT GACACTGGAG AGCTGATGGC TATGAAAGAA
    ATAAGATTTC AACCTAATGA TCACAAAACC ATCAAAGAAA CAGCTGATGA ACTGAAGATT
    TTTGAAGGCA TCAAACACCC TAATCTTGTT CGGTATTTTG GTGTGGAGCT CCATAGGGAA
    GAGATGTACA TCTTCATGGA GTACTGTGAT GAGGGCACGC TGGAGGAGGT GTCAAAACTG
    GGCCTTCAGG AGCATGTCAT TAGGCTCTAC ACAAAGCAGA TCACCATTGC TATCAATGTA
    CTACATGAAC ACGGTATTGT TCATCGAGAC ATTAAAGGAG CCAACATCTT TCTTACCTCA
    TCCGGACTGA TTAAACTGGG TGACTTTGGC TGCTCAGTAA AACTAAAGAA CAACACTCAA
    ACAATGCCTG GAGAAGTGAA CAGTACATTG GGTACTGCAG CGTACATGGC TCCAGAAGTA
    ATTACTAGAG CAAAAGGAGA AGGGCATGGA CGTGCAGCAG ACATCTGGAG CCTGGGCTGT
    GTTGTTATAG AGATGGTCAC TGGCAAGAGG CCTTGGCACG AATATGAGCA CAACTTCCAG
    ATCATGTATA GAGTGGGGAT GGGTCATAAA CCTCCTATTC CTGACAAAGT GAGTCCGGAG
    GGGAAAGACT TCCTTTGCCA CTGCCTGGAA AGTGACCCAA AGATGAGGTG GACAGCCAGC
    CAGCTCCTCG ACCATCCTTT TGTCAAGGTT TGCACAGATG AAGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.