LOC107055431 cDNA ORF clone, Gallus gallus(chicken)

The following LOC107055431 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC107055431 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa152893 XM_025144551.1
Latest version!
Gallus gallus E3 ubiquitin-protein ligase NEDD4-like (LOC107055431), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OGa152894 XM_025144552.1
Latest version!
Gallus gallus E3 ubiquitin-protein ligase NEDD4-like (LOC107055431), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OGa152895 XM_025144553.1
Latest version!
Gallus gallus E3 ubiquitin-protein ligase NEDD4-like (LOC107055431), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OGa152896 XM_025144554.1
Latest version!
Gallus gallus E3 ubiquitin-protein ligase NEDD4-like (LOC107055431), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OGa152897 XM_025144555.1
Latest version!
Gallus gallus E3 ubiquitin-protein ligase NEDD4-like (LOC107055431), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OGa152898 XM_025144556.1
Latest version!
Gallus gallus E3 ubiquitin-protein ligase NEDD4-like (LOC107055431), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OGa152899 XM_025144557.1
Latest version!
Gallus gallus E3 ubiquitin-protein ligase NEDD4-like (LOC107055431), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OGa47637 XM_015300422.1
Latest version!
Gallus gallus E3 ubiquitin-protein ligase NEDD4-like (LOC107055431), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OGa47637 XM_015300423.1
Latest version!
Gallus gallus E3 ubiquitin-protein ligase NEDD4-like (LOC107055431), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa152893
    Clone ID Related Accession (Same CDS sequence) XM_025144551.1
    Accession Version XM_025144551.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2916bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006126.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCGACTG CGGCGGTGGA GCCTGTGTAC GGGCTGGCGG AAGAGGAGGA GGAGTCTCGT 
    ATCCTCAGGG TGAAGGTGGT CTCTGGCATT GATCTGGCAA AAAAAGACAT CTTTGGAGCC
    AGTGATCCCT ACGTCAAACT TTCTTTGTAT GTAGCAGATG AGAACAGAGA ACTTTCTCTG
    GTGCAGACAA AAACTATTAA AAAGACACTG AATCCAAAAT GGAATGAGGA GTTTTATTTT
    AGAGTAAATC CAACCAATCA TCGCCTCCTG TTTGAAGTGT TTGATGAGAA CAGATTGACT
    AGAGATGACT TCCTGGGTCA GGTGGATTTG CCTCTTAGTC ACCTTCCGAC TGAAGACCCA
    ACCATGGAAA GGCCATACAC GTTTAAGGAT TTCCTTCTCA GACCAAGAAG CCACAAATCT
    CACGTAAAGG GTTTCTTGAG ATTGAAGATG GCTTATATGC CTAAAAATGG TGGTCAAGAG
    GAAGAAAATA GCAATCAAAG GAACGAGTCT GAGCATGAAT GGGATGTGAC TGATTCCAGT
    GACTCTGCAT CTCAGTGTCA GGAGGTGTTA CCACCTCCTC CACTGCCTCC TGGATGGGAA
    GAAAAGGTTG ACAACCTGGG GCGGACATAC TATGTCAACC ACAACAACAG AACTACTCAA
    TGGCATCGAC CAAGTTTAAT TGATGTGGGA TCCGATTCAG AGAACAACAG ACAAATAAAC
    TATGAAGCAG CCCATAGGCG GTTTCGCTCT CGGAGACACA TTAGTGAGGA TTTGGAACCA
    GAACCTATGG AGACTGGAGA CATTCTTGAG CCTTGGGAAG CCATTTCAGA GGAAGCAAGT
    ACAAGTGGAG ATTCTTTAAT TTTATCATTG CCACCACCTC CTGCATCTCC TGTATCCCAT
    GCCAGTCCTC AGGAGCTATC TGAGAAACTG AGCAGTAGAT TTCAGATCAC TAATGAGGAA
    CAGCTGAGTT CTTTGATTCA AAGAGATCCT TCTTCAAGAT TGAGATCTTG CAGTGTAACA
    GATACAGTCG CTGAACAGTC TCGCTTATCT TTGCAGGGAG GCCCATCTAG GAAAACTCGT
    TCTTCAGCTG TCACAGATGG TGAGGAAACA ATGCCTTCAG TGGCCTATGT CCACACTACA
    CCAGGCTTAC CTTCAGGCTG GGAAGAAAGA AAGGATGCAA AGGGCCGTAC TTACTATGTC
    AATCACAACA ATCGAACCAC AACCTGGACA CGGCCCATTA TACAGTTTAC AGAAGATGGC
    ATGGTTGGGT CAGCAGCAAA CAGCAACAGT CATTTAAGTG AACCTCAAAT CAGACGGCCT
    CGCAGCCTCA GTTCACCTAC AGTAACCTTA TCTGCTCCAC TTGAGGGTAT GAAGGACTCT
    CCAGTGCGCA GAGCAGTGAA GGACATACTA TCCAACCCAC AGTCTCTACA GCCCTCACCC
    TACAGCTCTC CTAAACCACA GAACAAAGTT GCACAAAGCT TCCTTCCTCC TGGCTGGGAG
    ATGCGAATAG CACCCAACAG CAGGCCCTTC TTCATCGATC ATAATACTAA AACTACTACA
    TGGGAAGATC CACGTCTGAA ATTTCCAGTG CATTTGAGAT CAAAAGCTTC TTTAAATCCT
    GATGATTTGG GCCCTCTTCC TCCTGGTTGG GAGGAAAGAA TACATTTGGA TGGAAGAATG
    TTTTATATAG ATCATAATAA TAAAATTACC CAATGGGAAG ATCCCAGACT GCAGAGCCCA
    GCAATTACTG GTCCAGCAGT TCCATATTCT AGGGAGTTCA AACAGAAATA TGACTATTTC
    CGGAAGAAGT TGAAGAAACC TGATGATATA CCAAGCAGGT TTGAGATGAA GCTGCACAGA
    AATAATATTT TTGAGGAGTC CTACAGAAGA ATCATGTCTG TGAAAAGACC TGATGTACTG
    AAGGCCAGGC TCTGGATTGA ATTTGAGTCA GAAAAAGGAC TTGACTATGG AGGTGTGGCT
    AGAGAATGGT TTTTTCTCTT GTCCAAGGAG ATGTTCAACC CATATTATGG TCTCTTTGAA
    TATTCAGCAA CGGACAACTA TACACTTCAG ATTAATCCTA ATTCAGGTCT TTGTAACGAA
    GATCATCTTT CATATTTCAT GTTTATTGGA CGAGTTGCTG GCCTGGCAGT GTATCATGGG
    AAACTATTGG ATGGCTTCTT CATCAGACCT TTTTACAAGA TGATGCTGGG GAAGCCAATA
    ATTCTGAAAG ATATGGAATC AGTGGACAGT GAATACTACA ACTCTTTGAA GTGGATCCTG
    GAAAATGACC CTACAGAGCT GGATCTCATG TTTTGCATTG ATGAGGAAAA CTTCGGACAG
    ACATATCAAG TAGACCTGAA GCCAAATGGG TCTGAAATCA TGGTAACAAA TGAAAACAAA
    AGAGAATATA TTGATCTGGT CATCCAGTGG CGGTTTGTAA ACAGAGTTCA AAAACAAATG
    AATGCTTTTT TGGAGGGATT CACAGAACTT CTTCCTATTG ATCTGATTAA AATTTTTGAT
    GAAAACGAAC TAGAGTTACT GATGTGTGGC CTTGGCGATG TTGACGTTAA TGACTGGAGA
    CAACATACAG TCTACAAAAA TGGCTACTGC CCAAATCATC CTGTTATCCA GTGGTTCTGG
    AAGGCTGTTT TACTGATGGA TGCAGAAAAA CGTATTCGGT TACTGCAGTT TGTTACTGGC
    ACATCACGTG TGCCTATGAA TGGATTTGCT GAACTTTATG GTTCAAATGG TCCTCAGCTG
    TTTACAATAG AGCAATGGGG AACTCCTGAT AAACTGCCCA GAGCTCACAC ATGCTTTAAT
    CGCTTAGACT TACCTCTTTA TGAATCTTTT GACAACCTAC AAGAGAAGCT ACTTATGGCA
    GTTGAAAATG CTGAAGGATT TGAAGGAGTG GATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025000319.1
    CDS3348..6263
    Translation

    Target ORF information:

    RefSeq Version XM_025144551.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus E3 ubiquitin-protein ligase NEDD4-like (LOC107055431), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025144551.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCGACTG CGGCGGTGGA GCCTGTGTAC GGGCTGGCGG AAGAGGAGGA GGAGTCTCGT 
    ATCCTCAGGG TGAAGGTGGT CTCTGGCATT GATCTGGCAA AAAAAGACAT CTTTGGAGCC
    AGTGATCCCT ACGTCAAACT TTCTTTGTAT GTAGCAGATG AGAACAGAGA ACTTTCTCTG
    GTGCAGACAA AAACTATTAA AAAGACACTG AATCCAAAAT GGAATGAGGA GTTTTATTTT
    AGAGTAAATC CAACCAATCA TCGCCTCCTG TTTGAAGTGT TTGATGAGAA CAGATTGACT
    AGAGATGACT TCCTGGGTCA GGTGGATTTG CCTCTTAGTC ACCTTCCGAC TGAAGACCCA
    ACCATGGAAA GGCCATACAC GTTTAAGGAT TTCCTTCTCA GACCAAGAAG CCACAAATCT
    CACGTAAAGG GTTTCTTGAG ATTGAAGATG GCTTATATGC CTAAAAATGG TGGTCAAGAG
    GAAGAAAATA GCAATCAAAG GAACGAGTCT GAGCATGAAT GGGATGTGAC TGATTCCAGT
    GACTCTGCAT CTCAGTGTCA GGAGGTGTTA CCACCTCCTC CACTGCCTCC TGGATGGGAA
    GAAAAGGTTG ACAACCTGGG GCGGACATAC TATGTCAACC ACAACAACAG AACTACTCAA
    TGGCATCGAC CAAGTTTAAT TGATGTGGGA TCCGATTCAG AGAACAACAG ACAAATAAAC
    TATGAAGCAG CCCATAGGCG GTTTCGCTCT CGGAGACACA TTAGTGAGGA TTTGGAACCA
    GAACCTATGG AGACTGGAGA CATTCTTGAG CCTTGGGAAG CCATTTCAGA GGAAGCAAGT
    ACAAGTGGAG ATTCTTTAAT TTTATCATTG CCACCACCTC CTGCATCTCC TGTATCCCAT
    GCCAGTCCTC AGGAGCTATC TGAGAAACTG AGCAGTAGAT TTCAGATCAC TAATGAGGAA
    CAGCTGAGTT CTTTGATTCA AAGAGATCCT TCTTCAAGAT TGAGATCTTG CAGTGTAACA
    GATACAGTCG CTGAACAGTC TCGCTTATCT TTGCAGGGAG GCCCATCTAG GAAAACTCGT
    TCTTCAGCTG TCACAGATGG TGAGGAAACA ATGCCTTCAG TGGCCTATGT CCACACTACA
    CCAGGCTTAC CTTCAGGCTG GGAAGAAAGA AAGGATGCAA AGGGCCGTAC TTACTATGTC
    AATCACAACA ATCGAACCAC AACCTGGACA CGGCCCATTA TACAGTTTAC AGAAGATGGC
    ATGGTTGGGT CAGCAGCAAA CAGCAACAGT CATTTAAGTG AACCTCAAAT CAGACGGCCT
    CGCAGCCTCA GTTCACCTAC AGTAACCTTA TCTGCTCCAC TTGAGGGTAT GAAGGACTCT
    CCAGTGCGCA GAGCAGTGAA GGACATACTA TCCAACCCAC AGTCTCTACA GCCCTCACCC
    TACAGCTCTC CTAAACCACA GAACAAAGTT GCACAAAGCT TCCTTCCTCC TGGCTGGGAG
    ATGCGAATAG CACCCAACAG CAGGCCCTTC TTCATCGATC ATAATACTAA AACTACTACA
    TGGGAAGATC CACGTCTGAA ATTTCCAGTG CATTTGAGAT CAAAAGCTTC TTTAAATCCT
    GATGATTTGG GCCCTCTTCC TCCTGGTTGG GAGGAAAGAA TACATTTGGA TGGAAGAATG
    TTTTATATAG ATCATAATAA TAAAATTACC CAATGGGAAG ATCCCAGACT GCAGAGCCCA
    GCAATTACTG GTCCAGCAGT TCCATATTCT AGGGAGTTCA AACAGAAATA TGACTATTTC
    CGGAAGAAGT TGAAGAAACC TGATGATATA CCAAGCAGGT TTGAGATGAA GCTGCACAGA
    AATAATATTT TTGAGGAGTC CTACAGAAGA ATCATGTCTG TGAAAAGACC TGATGTACTG
    AAGGCCAGGC TCTGGATTGA ATTTGAGTCA GAAAAAGGAC TTGACTATGG AGGTGTGGCT
    AGAGAATGGT TTTTTCTCTT GTCCAAGGAG ATGTTCAACC CATATTATGG TCTCTTTGAA
    TATTCAGCAA CGGACAACTA TACACTTCAG ATTAATCCTA ATTCAGGTCT TTGTAACGAA
    GATCATCTTT CATATTTCAT GTTTATTGGA CGAGTTGCTG GCCTGGCAGT GTATCATGGG
    AAACTATTGG ATGGCTTCTT CATCAGACCT TTTTACAAGA TGATGCTGGG GAAGCCAATA
    ATTCTGAAAG ATATGGAATC AGTGGACAGT GAATACTACA ACTCTTTGAA GTGGATCCTG
    GAAAATGACC CTACAGAGCT GGATCTCATG TTTTGCATTG ATGAGGAAAA CTTCGGACAG
    ACATATCAAG TAGACCTGAA GCCAAATGGG TCTGAAATCA TGGTAACAAA TGAAAACAAA
    AGAGAATATA TTGATCTGGT CATCCAGTGG CGGTTTGTAA ACAGAGTTCA AAAACAAATG
    AATGCTTTTT TGGAGGGATT CACAGAACTT CTTCCTATTG ATCTGATTAA AATTTTTGAT
    GAAAACGAAC TAGAGTTACT GATGTGTGGC CTTGGCGATG TTGACGTTAA TGACTGGAGA
    CAACATACAG TCTACAAAAA TGGCTACTGC CCAAATCATC CTGTTATCCA GTGGTTCTGG
    AAGGCTGTTT TACTGATGGA TGCAGAAAAA CGTATTCGGT TACTGCAGTT TGTTACTGGC
    ACATCACGTG TGCCTATGAA TGGATTTGCT GAACTTTATG GTTCAAATGG TCCTCAGCTG
    TTTACAATAG AGCAATGGGG AACTCCTGAT AAACTGCCCA GAGCTCACAC ATGCTTTAAT
    CGCTTAGACT TACCTCTTTA TGAATCTTTT GACAACCTAC AAGAGAAGCT ACTTATGGCA
    GTTGAAAATG CTGAAGGATT TGAAGGAGTG GATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152894
    Clone ID Related Accession (Same CDS sequence) XM_025144552.1
    Accession Version XM_025144552.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2913bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006126.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGATGGAGA TGCCAGTTTT CTTCTGGGGA AAGGCCTCGG TGGAGGAGGA GTCTCGTATC 
    CTCAGGGTGA AGGTGGTCTC TGGCATTGAT CTGGCAAAAA AAGACATCTT TGGAGCCAGT
    GATCCCTACG TCAAACTTTC TTTGTATGTA GCAGATGAGA ACAGAGAACT TTCTCTGGTG
    CAGACAAAAA CTATTAAAAA GACACTGAAT CCAAAATGGA ATGAGGAGTT TTATTTTAGA
    GTAAATCCAA CCAATCATCG CCTCCTGTTT GAAGTGTTTG ATGAGAACAG ATTGACTAGA
    GATGACTTCC TGGGTCAGGT GGATTTGCCT CTTAGTCACC TTCCGACTGA AGACCCAACC
    ATGGAAAGGC CATACACGTT TAAGGATTTC CTTCTCAGAC CAAGAAGCCA CAAATCTCAC
    GTAAAGGGTT TCTTGAGATT GAAGATGGCT TATATGCCTA AAAATGGTGG TCAAGAGGAA
    GAAAATAGCA ATCAAAGGAA CGAGTCTGAG CATGAATGGG ATGTGACTGA TTCCAGTGAC
    TCTGCATCTC AGTGTCAGGA GGTGTTACCA CCTCCTCCAC TGCCTCCTGG ATGGGAAGAA
    AAGGTTGACA ACCTGGGGCG GACATACTAT GTCAACCACA ACAACAGAAC TACTCAATGG
    CATCGACCAA GTTTAATTGA TGTGGGATCC GATTCAGAGA ACAACAGACA AATAAACTAT
    GAAGCAGCCC ATAGGCGGTT TCGCTCTCGG AGACACATTA GTGAGGATTT GGAACCAGAA
    CCTATGGAGA CTGGAGACAT TCTTGAGCCT TGGGAAGCCA TTTCAGAGGA AGCAAGTACA
    AGTGGAGATT CTTTAATTTT ATCATTGCCA CCACCTCCTG CATCTCCTGT ATCCCATGCC
    AGTCCTCAGG AGCTATCTGA GAAACTGAGC AGTAGATTTC AGATCACTAA TGAGGAACAG
    CTGAGTTCTT TGATTCAAAG AGATCCTTCT TCAAGATTGA GATCTTGCAG TGTAACAGAT
    ACAGTCGCTG AACAGTCTCG CTTATCTTTG CAGGGAGGCC CATCTAGGAA AACTCGTTCT
    TCAGCTGTCA CAGATGGTGA GGAAACAATG CCTTCAGTGG CCTATGTCCA CACTACACCA
    GGCTTACCTT CAGGCTGGGA AGAAAGAAAG GATGCAAAGG GCCGTACTTA CTATGTCAAT
    CACAACAATC GAACCACAAC CTGGACACGG CCCATTATAC AGTTTACAGA AGATGGCATG
    GTTGGGTCAG CAGCAAACAG CAACAGTCAT TTAAGTGAAC CTCAAATCAG ACGGCCTCGC
    AGCCTCAGTT CACCTACAGT AACCTTATCT GCTCCACTTG AGGGTATGAA GGACTCTCCA
    GTGCGCAGAG CAGTGAAGGA CATACTATCC AACCCACAGT CTCTACAGCC CTCACCCTAC
    AGCTCTCCTA AACCACAGAA CAAAGTTGCA CAAAGCTTCC TTCCTCCTGG CTGGGAGATG
    CGAATAGCAC CCAACAGCAG GCCCTTCTTC ATCGATCATA ATACTAAAAC TACTACATGG
    GAAGATCCAC GTCTGAAATT TCCAGTGCAT TTGAGATCAA AAGCTTCTTT AAATCCTGAT
    GATTTGGGCC CTCTTCCTCC TGGTTGGGAG GAAAGAATAC ATTTGGATGG AAGAATGTTT
    TATATAGATC ATAATAATAA AATTACCCAA TGGGAAGATC CCAGACTGCA GAGCCCAGCA
    ATTACTGGTC CAGCAGTTCC ATATTCTAGG GAGTTCAAAC AGAAATATGA CTATTTCCGG
    AAGAAGTTGA AGAAACCTGA TGATATACCA AGCAGGTTTG AGATGAAGCT GCACAGAAAT
    AATATTTTTG AGGAGTCCTA CAGAAGAATC ATGTCTGTGA AAAGACCTGA TGTACTGAAG
    GCCAGGCTCT GGATTGAATT TGAGTCAGAA AAAGGACTTG ACTATGGAGG TGTGGCTAGA
    GAATGGTTTT TTCTCTTGTC CAAGGAGATG TTCAACCCAT ATTATGGTCT CTTTGAATAT
    TCAGCAACGG ACAACTATAC ACTTCAGATT AATCCTAATT CAGGTCTTTG TAACGAAGAT
    CATCTTTCAT ATTTCATGTT TATTGGACGA GTTGCTGGCC TGGCAGTGTA TCATGGGAAA
    CTATTGGATG GCTTCTTCAT CAGACCTTTT TACAAGATGA TGCTGGGGAA GCCAATAATT
    CTGAAAGATA TGGAATCAGT GGACAGTGAA TACTACAACT CTTTGAAGTG GATCCTGGAA
    AATGACCCTA CAGAGCTGGA TCTCATGTTT TGCATTGATG AGGAAAACTT CGGACAGACA
    TATCAAGTAG ACCTGAAGCC AAATGGGTCT GAAATCATGG TAACAAATGA AAACAAAAGA
    GAATATATTG ATCTGGTCAT CCAGTGGCGG TTTGTAAACA GAGTTCAAAA ACAAATGAAT
    GCTTTTTTGG AGGGATTCAC AGAACTTCTT CCTATTGATC TGATTAAAAT TTTTGATGAA
    AACGAACTAG AGTTACTGAT GTGTGGCCTT GGCGATGTTG ACGTTAATGA CTGGAGACAA
    CATACAGTCT ACAAAAATGG CTACTGCCCA AATCATCCTG TTATCCAGTG GTTCTGGAAG
    GCTGTTTTAC TGATGGATGC AGAAAAACGT ATTCGGTTAC TGCAGTTTGT TACTGGCACA
    TCACGTGTGC CTATGAATGG ATTTGCTGAA CTTTATGGTT CAAATGGTCC TCAGCTGTTT
    ACAATAGAGC AATGGGGAAC TCCTGATAAA CTGCCCAGAG CTCACACATG CTTTAATCGC
    TTAGACTTAC CTCTTTATGA ATCTTTTGAC AACCTACAAG AGAAGCTACT TATGGCAGTT
    GAAAATGCTG AAGGATTTGA AGGAGTGGAT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025000320.1
    CDS3431..6343
    Translation

    Target ORF information:

    RefSeq Version XM_025144552.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus E3 ubiquitin-protein ligase NEDD4-like (LOC107055431), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025144552.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGATGGAGA TGCCAGTTTT CTTCTGGGGA AAGGCCTCGG TGGAGGAGGA GTCTCGTATC 
    CTCAGGGTGA AGGTGGTCTC TGGCATTGAT CTGGCAAAAA AAGACATCTT TGGAGCCAGT
    GATCCCTACG TCAAACTTTC TTTGTATGTA GCAGATGAGA ACAGAGAACT TTCTCTGGTG
    CAGACAAAAA CTATTAAAAA GACACTGAAT CCAAAATGGA ATGAGGAGTT TTATTTTAGA
    GTAAATCCAA CCAATCATCG CCTCCTGTTT GAAGTGTTTG ATGAGAACAG ATTGACTAGA
    GATGACTTCC TGGGTCAGGT GGATTTGCCT CTTAGTCACC TTCCGACTGA AGACCCAACC
    ATGGAAAGGC CATACACGTT TAAGGATTTC CTTCTCAGAC CAAGAAGCCA CAAATCTCAC
    GTAAAGGGTT TCTTGAGATT GAAGATGGCT TATATGCCTA AAAATGGTGG TCAAGAGGAA
    GAAAATAGCA ATCAAAGGAA CGAGTCTGAG CATGAATGGG ATGTGACTGA TTCCAGTGAC
    TCTGCATCTC AGTGTCAGGA GGTGTTACCA CCTCCTCCAC TGCCTCCTGG ATGGGAAGAA
    AAGGTTGACA ACCTGGGGCG GACATACTAT GTCAACCACA ACAACAGAAC TACTCAATGG
    CATCGACCAA GTTTAATTGA TGTGGGATCC GATTCAGAGA ACAACAGACA AATAAACTAT
    GAAGCAGCCC ATAGGCGGTT TCGCTCTCGG AGACACATTA GTGAGGATTT GGAACCAGAA
    CCTATGGAGA CTGGAGACAT TCTTGAGCCT TGGGAAGCCA TTTCAGAGGA AGCAAGTACA
    AGTGGAGATT CTTTAATTTT ATCATTGCCA CCACCTCCTG CATCTCCTGT ATCCCATGCC
    AGTCCTCAGG AGCTATCTGA GAAACTGAGC AGTAGATTTC AGATCACTAA TGAGGAACAG
    CTGAGTTCTT TGATTCAAAG AGATCCTTCT TCAAGATTGA GATCTTGCAG TGTAACAGAT
    ACAGTCGCTG AACAGTCTCG CTTATCTTTG CAGGGAGGCC CATCTAGGAA AACTCGTTCT
    TCAGCTGTCA CAGATGGTGA GGAAACAATG CCTTCAGTGG CCTATGTCCA CACTACACCA
    GGCTTACCTT CAGGCTGGGA AGAAAGAAAG GATGCAAAGG GCCGTACTTA CTATGTCAAT
    CACAACAATC GAACCACAAC CTGGACACGG CCCATTATAC AGTTTACAGA AGATGGCATG
    GTTGGGTCAG CAGCAAACAG CAACAGTCAT TTAAGTGAAC CTCAAATCAG ACGGCCTCGC
    AGCCTCAGTT CACCTACAGT AACCTTATCT GCTCCACTTG AGGGTATGAA GGACTCTCCA
    GTGCGCAGAG CAGTGAAGGA CATACTATCC AACCCACAGT CTCTACAGCC CTCACCCTAC
    AGCTCTCCTA AACCACAGAA CAAAGTTGCA CAAAGCTTCC TTCCTCCTGG CTGGGAGATG
    CGAATAGCAC CCAACAGCAG GCCCTTCTTC ATCGATCATA ATACTAAAAC TACTACATGG
    GAAGATCCAC GTCTGAAATT TCCAGTGCAT TTGAGATCAA AAGCTTCTTT AAATCCTGAT
    GATTTGGGCC CTCTTCCTCC TGGTTGGGAG GAAAGAATAC ATTTGGATGG AAGAATGTTT
    TATATAGATC ATAATAATAA AATTACCCAA TGGGAAGATC CCAGACTGCA GAGCCCAGCA
    ATTACTGGTC CAGCAGTTCC ATATTCTAGG GAGTTCAAAC AGAAATATGA CTATTTCCGG
    AAGAAGTTGA AGAAACCTGA TGATATACCA AGCAGGTTTG AGATGAAGCT GCACAGAAAT
    AATATTTTTG AGGAGTCCTA CAGAAGAATC ATGTCTGTGA AAAGACCTGA TGTACTGAAG
    GCCAGGCTCT GGATTGAATT TGAGTCAGAA AAAGGACTTG ACTATGGAGG TGTGGCTAGA
    GAATGGTTTT TTCTCTTGTC CAAGGAGATG TTCAACCCAT ATTATGGTCT CTTTGAATAT
    TCAGCAACGG ACAACTATAC ACTTCAGATT AATCCTAATT CAGGTCTTTG TAACGAAGAT
    CATCTTTCAT ATTTCATGTT TATTGGACGA GTTGCTGGCC TGGCAGTGTA TCATGGGAAA
    CTATTGGATG GCTTCTTCAT CAGACCTTTT TACAAGATGA TGCTGGGGAA GCCAATAATT
    CTGAAAGATA TGGAATCAGT GGACAGTGAA TACTACAACT CTTTGAAGTG GATCCTGGAA
    AATGACCCTA CAGAGCTGGA TCTCATGTTT TGCATTGATG AGGAAAACTT CGGACAGACA
    TATCAAGTAG ACCTGAAGCC AAATGGGTCT GAAATCATGG TAACAAATGA AAACAAAAGA
    GAATATATTG ATCTGGTCAT CCAGTGGCGG TTTGTAAACA GAGTTCAAAA ACAAATGAAT
    GCTTTTTTGG AGGGATTCAC AGAACTTCTT CCTATTGATC TGATTAAAAT TTTTGATGAA
    AACGAACTAG AGTTACTGAT GTGTGGCCTT GGCGATGTTG ACGTTAATGA CTGGAGACAA
    CATACAGTCT ACAAAAATGG CTACTGCCCA AATCATCCTG TTATCCAGTG GTTCTGGAAG
    GCTGTTTTAC TGATGGATGC AGAAAAACGT ATTCGGTTAC TGCAGTTTGT TACTGGCACA
    TCACGTGTGC CTATGAATGG ATTTGCTGAA CTTTATGGTT CAAATGGTCC TCAGCTGTTT
    ACAATAGAGC AATGGGGAAC TCCTGATAAA CTGCCCAGAG CTCACACATG CTTTAATCGC
    TTAGACTTAC CTCTTTATGA ATCTTTTGAC AACCTACAAG AGAAGCTACT TATGGCAGTT
    GAAAATGCTG AAGGATTTGA AGGAGTGGAT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152895
    Clone ID Related Accession (Same CDS sequence) XM_025144553.1
    Accession Version XM_025144553.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2865bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006126.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCGACTG CGGCGGTGGA GCCTGTGTAC GGGCTGGCGG AAGAGGAGGA GGAGTCTCGT 
    ATCCTCAGGG TGAAGGTGGT CTCTGGCATT GATCTGGCAA AAAAAGACAT CTTTGGAGCC
    AGTGATCCCT ACGTCAAACT TTCTTTGTAT GTAGCAGATG AGAACAGAGA ACTTTCTCTG
    GTGCAGACAA AAACTATTAA AAAGACACTG AATCCAAAAT GGAATGAGGA GTTTTATTTT
    AGAGTAAATC CAACCAATCA TCGCCTCCTG TTTGAAGTGT TTGATGAGAA CAGATTGACT
    GAAGACCCAA CCATGGAAAG GCCATACACG TTTAAGGATT TCCTTCTCAG ACCAAGAAGC
    CACAAATCTC ACGTAAAGGG TTTCTTGAGA TTGAAGATGG CTTATATGCC TAAAAATGGT
    GGTCAAGAGG AAGAAAATAG CAATCAAAGG AACGAGTCTG AGCATGAATG GGATGTGACT
    GATTCCAGTG ACTCTGCATC TCAGTGTCAG GAGGTGTTAC CACCTCCTCC ACTGCCTCCT
    GGATGGGAAG AAAAGGTTGA CAACCTGGGG CGGACATACT ATGTCAACCA CAACAACAGA
    ACTACTCAAT GGCATCGACC AAGTTTAATT GATGTGGGAT CCGATTCAGA GAACAACAGA
    CAAATAAACT ATGAAGCAGC CCATAGGCGG TTTCGCTCTC GGAGACACAT TAGTGAGGAT
    TTGGAACCAG AACCTATGGA GACTGGAGAC ATTCTTGAGC CTTGGGAAGC CATTTCAGAG
    GAAGCAAGTA CAAGTGGAGA TTCTTTAATT TTATCATTGC CACCACCTCC TGCATCTCCT
    GTATCCCATG CCAGTCCTCA GGAGCTATCT GAGAAACTGA GCAGTAGATT TCAGATCACT
    AATGAGGAAC AGCTGAGTTC TTTGATTCAA AGAGATCCTT CTTCAAGATT GAGATCTTGC
    AGTGTAACAG ATACAGTCGC TGAACAGTCT CGCTTATCTT TGCAGGGAGG CCCATCTAGG
    AAAACTCGTT CTTCAGCTGT CACAGATGGT GAGGAAACAA TGCCTTCAGT GGCCTATGTC
    CACACTACAC CAGGCTTACC TTCAGGCTGG GAAGAAAGAA AGGATGCAAA GGGCCGTACT
    TACTATGTCA ATCACAACAA TCGAACCACA ACCTGGACAC GGCCCATTAT ACAGTTTACA
    GAAGATGGCA TGGTTGGGTC AGCAGCAAAC AGCAACAGTC ATTTAAGTGA ACCTCAAATC
    AGACGGCCTC GCAGCCTCAG TTCACCTACA GTAACCTTAT CTGCTCCACT TGAGGGTATG
    AAGGACTCTC CAGTGCGCAG AGCAGTGAAG GACATACTAT CCAACCCACA GTCTCTACAG
    CCCTCACCCT ACAGCTCTCC TAAACCACAG AACAAAGTTG CACAAAGCTT CCTTCCTCCT
    GGCTGGGAGA TGCGAATAGC ACCCAACAGC AGGCCCTTCT TCATCGATCA TAATACTAAA
    ACTACTACAT GGGAAGATCC ACGTCTGAAA TTTCCAGTGC ATTTGAGATC AAAAGCTTCT
    TTAAATCCTG ATGATTTGGG CCCTCTTCCT CCTGGTTGGG AGGAAAGAAT ACATTTGGAT
    GGAAGAATGT TTTATATAGA TCATAATAAT AAAATTACCC AATGGGAAGA TCCCAGACTG
    CAGAGCCCAG CAATTACTGG TCCAGCAGTT CCATATTCTA GGGAGTTCAA ACAGAAATAT
    GACTATTTCC GGAAGAAGTT GAAGAAACCT GATGATATAC CAAGCAGGTT TGAGATGAAG
    CTGCACAGAA ATAATATTTT TGAGGAGTCC TACAGAAGAA TCATGTCTGT GAAAAGACCT
    GATGTACTGA AGGCCAGGCT CTGGATTGAA TTTGAGTCAG AAAAAGGACT TGACTATGGA
    GGTGTGGCTA GAGAATGGTT TTTTCTCTTG TCCAAGGAGA TGTTCAACCC ATATTATGGT
    CTCTTTGAAT ATTCAGCAAC GGACAACTAT ACACTTCAGA TTAATCCTAA TTCAGGTCTT
    TGTAACGAAG ATCATCTTTC ATATTTCATG TTTATTGGAC GAGTTGCTGG CCTGGCAGTG
    TATCATGGGA AACTATTGGA TGGCTTCTTC ATCAGACCTT TTTACAAGAT GATGCTGGGG
    AAGCCAATAA TTCTGAAAGA TATGGAATCA GTGGACAGTG AATACTACAA CTCTTTGAAG
    TGGATCCTGG AAAATGACCC TACAGAGCTG GATCTCATGT TTTGCATTGA TGAGGAAAAC
    TTCGGACAGA CATATCAAGT AGACCTGAAG CCAAATGGGT CTGAAATCAT GGTAACAAAT
    GAAAACAAAA GAGAATATAT TGATCTGGTC ATCCAGTGGC GGTTTGTAAA CAGAGTTCAA
    AAACAAATGA ATGCTTTTTT GGAGGGATTC ACAGAACTTC TTCCTATTGA TCTGATTAAA
    ATTTTTGATG AAAACGAACT AGAGTTACTG ATGTGTGGCC TTGGCGATGT TGACGTTAAT
    GACTGGAGAC AACATACAGT CTACAAAAAT GGCTACTGCC CAAATCATCC TGTTATCCAG
    TGGTTCTGGA AGGCTGTTTT ACTGATGGAT GCAGAAAAAC GTATTCGGTT ACTGCAGTTT
    GTTACTGGCA CATCACGTGT GCCTATGAAT GGATTTGCTG AACTTTATGG TTCAAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGA ACTCCTGATA AACTGCCCAG AGCTCACACA
    TGCTTTAATC GCTTAGACTT ACCTCTTTAT GAATCTTTTG ACAACCTACA AGAGAAGCTA
    CTTATGGCAG TTGAAAATGC TGAAGGATTT GAAGGAGTGG ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025000321.1
    CDS3349..6213
    Translation

    Target ORF information:

    RefSeq Version XM_025144553.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus E3 ubiquitin-protein ligase NEDD4-like (LOC107055431), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025144553.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCGACTG CGGCGGTGGA GCCTGTGTAC GGGCTGGCGG AAGAGGAGGA GGAGTCTCGT 
    ATCCTCAGGG TGAAGGTGGT CTCTGGCATT GATCTGGCAA AAAAAGACAT CTTTGGAGCC
    AGTGATCCCT ACGTCAAACT TTCTTTGTAT GTAGCAGATG AGAACAGAGA ACTTTCTCTG
    GTGCAGACAA AAACTATTAA AAAGACACTG AATCCAAAAT GGAATGAGGA GTTTTATTTT
    AGAGTAAATC CAACCAATCA TCGCCTCCTG TTTGAAGTGT TTGATGAGAA CAGATTGACT
    GAAGACCCAA CCATGGAAAG GCCATACACG TTTAAGGATT TCCTTCTCAG ACCAAGAAGC
    CACAAATCTC ACGTAAAGGG TTTCTTGAGA TTGAAGATGG CTTATATGCC TAAAAATGGT
    GGTCAAGAGG AAGAAAATAG CAATCAAAGG AACGAGTCTG AGCATGAATG GGATGTGACT
    GATTCCAGTG ACTCTGCATC TCAGTGTCAG GAGGTGTTAC CACCTCCTCC ACTGCCTCCT
    GGATGGGAAG AAAAGGTTGA CAACCTGGGG CGGACATACT ATGTCAACCA CAACAACAGA
    ACTACTCAAT GGCATCGACC AAGTTTAATT GATGTGGGAT CCGATTCAGA GAACAACAGA
    CAAATAAACT ATGAAGCAGC CCATAGGCGG TTTCGCTCTC GGAGACACAT TAGTGAGGAT
    TTGGAACCAG AACCTATGGA GACTGGAGAC ATTCTTGAGC CTTGGGAAGC CATTTCAGAG
    GAAGCAAGTA CAAGTGGAGA TTCTTTAATT TTATCATTGC CACCACCTCC TGCATCTCCT
    GTATCCCATG CCAGTCCTCA GGAGCTATCT GAGAAACTGA GCAGTAGATT TCAGATCACT
    AATGAGGAAC AGCTGAGTTC TTTGATTCAA AGAGATCCTT CTTCAAGATT GAGATCTTGC
    AGTGTAACAG ATACAGTCGC TGAACAGTCT CGCTTATCTT TGCAGGGAGG CCCATCTAGG
    AAAACTCGTT CTTCAGCTGT CACAGATGGT GAGGAAACAA TGCCTTCAGT GGCCTATGTC
    CACACTACAC CAGGCTTACC TTCAGGCTGG GAAGAAAGAA AGGATGCAAA GGGCCGTACT
    TACTATGTCA ATCACAACAA TCGAACCACA ACCTGGACAC GGCCCATTAT ACAGTTTACA
    GAAGATGGCA TGGTTGGGTC AGCAGCAAAC AGCAACAGTC ATTTAAGTGA ACCTCAAATC
    AGACGGCCTC GCAGCCTCAG TTCACCTACA GTAACCTTAT CTGCTCCACT TGAGGGTATG
    AAGGACTCTC CAGTGCGCAG AGCAGTGAAG GACATACTAT CCAACCCACA GTCTCTACAG
    CCCTCACCCT ACAGCTCTCC TAAACCACAG AACAAAGTTG CACAAAGCTT CCTTCCTCCT
    GGCTGGGAGA TGCGAATAGC ACCCAACAGC AGGCCCTTCT TCATCGATCA TAATACTAAA
    ACTACTACAT GGGAAGATCC ACGTCTGAAA TTTCCAGTGC ATTTGAGATC AAAAGCTTCT
    TTAAATCCTG ATGATTTGGG CCCTCTTCCT CCTGGTTGGG AGGAAAGAAT ACATTTGGAT
    GGAAGAATGT TTTATATAGA TCATAATAAT AAAATTACCC AATGGGAAGA TCCCAGACTG
    CAGAGCCCAG CAATTACTGG TCCAGCAGTT CCATATTCTA GGGAGTTCAA ACAGAAATAT
    GACTATTTCC GGAAGAAGTT GAAGAAACCT GATGATATAC CAAGCAGGTT TGAGATGAAG
    CTGCACAGAA ATAATATTTT TGAGGAGTCC TACAGAAGAA TCATGTCTGT GAAAAGACCT
    GATGTACTGA AGGCCAGGCT CTGGATTGAA TTTGAGTCAG AAAAAGGACT TGACTATGGA
    GGTGTGGCTA GAGAATGGTT TTTTCTCTTG TCCAAGGAGA TGTTCAACCC ATATTATGGT
    CTCTTTGAAT ATTCAGCAAC GGACAACTAT ACACTTCAGA TTAATCCTAA TTCAGGTCTT
    TGTAACGAAG ATCATCTTTC ATATTTCATG TTTATTGGAC GAGTTGCTGG CCTGGCAGTG
    TATCATGGGA AACTATTGGA TGGCTTCTTC ATCAGACCTT TTTACAAGAT GATGCTGGGG
    AAGCCAATAA TTCTGAAAGA TATGGAATCA GTGGACAGTG AATACTACAA CTCTTTGAAG
    TGGATCCTGG AAAATGACCC TACAGAGCTG GATCTCATGT TTTGCATTGA TGAGGAAAAC
    TTCGGACAGA CATATCAAGT AGACCTGAAG CCAAATGGGT CTGAAATCAT GGTAACAAAT
    GAAAACAAAA GAGAATATAT TGATCTGGTC ATCCAGTGGC GGTTTGTAAA CAGAGTTCAA
    AAACAAATGA ATGCTTTTTT GGAGGGATTC ACAGAACTTC TTCCTATTGA TCTGATTAAA
    ATTTTTGATG AAAACGAACT AGAGTTACTG ATGTGTGGCC TTGGCGATGT TGACGTTAAT
    GACTGGAGAC AACATACAGT CTACAAAAAT GGCTACTGCC CAAATCATCC TGTTATCCAG
    TGGTTCTGGA AGGCTGTTTT ACTGATGGAT GCAGAAAAAC GTATTCGGTT ACTGCAGTTT
    GTTACTGGCA CATCACGTGT GCCTATGAAT GGATTTGCTG AACTTTATGG TTCAAATGGT
    CCTCAGCTGT TTACAATAGA GCAATGGGGA ACTCCTGATA AACTGCCCAG AGCTCACACA
    TGCTTTAATC GCTTAGACTT ACCTCTTTAT GAATCTTTTG ACAACCTACA AGAGAAGCTA
    CTTATGGCAG TTGAAAATGC TGAAGGATTT GAAGGAGTGG ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152896
    Clone ID Related Accession (Same CDS sequence) XM_025144554.1
    Accession Version XM_025144554.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2856bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006126.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCGACTG CGGCGGTGGA GCCTGTGTAC GGGCTGGCGG AAGAGGAGGA GGAGTCTCGT 
    ATCCTCAGGG TGAAGGTGGT CTCTGGCATT GATCTGGCAA AAAAAGACAT CTTTGGAGCC
    AGTGATCCCT ACGTCAAACT TTCTTTGTAT GTAGCAGATG AGAACAGAGA ACTTTCTCTG
    GTGCAGACAA AAACTATTAA AAAGACACTG AATCCAAAAT GGAATGAGGA GTTTTATTTT
    AGAGTAAATC CAACCAATCA TCGCCTCCTG TTTGAAGTGT TTGATGAGAA CAGATTGACT
    AGAGATGACT TCCTGGGTCA GGTGGATTTG CCTCTTAGTC ACCTTCCGAC TGAAGACCCA
    ACCATGGAAA GGCCATACAC GTTTAAGGAT TTCCTTCTCA GACCAAGAAG CCACAAATCT
    CACGTAAAGG GTTTCTTGAG ATTGAAGATG GCTTATATGC CTAAAAATGG TGGTCAAGAG
    GAAGAAAATA GCAATCAAAG GAACGAGTCT GAGCATGAAT GGGATGTGAC TGATTCCAGT
    GACTCTGCAT CTCAGTGTCA GGAGGTGTTA CCACCTCCTC CACTGCCTCC TGGATGGGAA
    GAAAAGGTTG ACAACCTGGG GCGGACATAC TATGTCAACC ACAACAACAG AACTACTCAA
    TGGCATCGAC CAAGTTTAAT TGATGTGGGA TCCGATTCAG AGAACAACAG ACAAATAAAC
    TATGAAGCAG CCCATAGGCG GTTTCGCTCT CGGAGACACA TTAGTGAGGA TTTGGAACCA
    GAACCTATGG AGACTGGAGA CATTCTTGAG CCTTGGGAAG CCATTTCAGA GGAAGCAAGT
    ACAAGTGGAG ATTCTTTAAT TTTATCATTG CCACCACCTC CTGCATCTCC TGTATCCCAT
    GCCAGTCCTC AGGAGCTATC TGAGAAACTG AGCAGTAGAT TTCAGATCAC TAATGAGGAA
    CAGCTGAGTT CTTTGATTCA AAGAGATCCT TCTTCAAGAT TGAGATCTTG CAGTGTAACA
    GATACAGTCG CTGAACAGTC TCGCTTATCT TTGCCTTCAG TGGCCTATGT CCACACTACA
    CCAGGCTTAC CTTCAGGCTG GGAAGAAAGA AAGGATGCAA AGGGCCGTAC TTACTATGTC
    AATCACAACA ATCGAACCAC AACCTGGACA CGGCCCATTA TACAGTTTAC AGAAGATGGC
    ATGGTTGGGT CAGCAGCAAA CAGCAACAGT CATTTAAGTG AACCTCAAAT CAGACGGCCT
    CGCAGCCTCA GTTCACCTAC AGTAACCTTA TCTGCTCCAC TTGAGGGTAT GAAGGACTCT
    CCAGTGCGCA GAGCAGTGAA GGACATACTA TCCAACCCAC AGTCTCTACA GCCCTCACCC
    TACAGCTCTC CTAAACCACA GAACAAAGTT GCACAAAGCT TCCTTCCTCC TGGCTGGGAG
    ATGCGAATAG CACCCAACAG CAGGCCCTTC TTCATCGATC ATAATACTAA AACTACTACA
    TGGGAAGATC CACGTCTGAA ATTTCCAGTG CATTTGAGAT CAAAAGCTTC TTTAAATCCT
    GATGATTTGG GCCCTCTTCC TCCTGGTTGG GAGGAAAGAA TACATTTGGA TGGAAGAATG
    TTTTATATAG ATCATAATAA TAAAATTACC CAATGGGAAG ATCCCAGACT GCAGAGCCCA
    GCAATTACTG GTCCAGCAGT TCCATATTCT AGGGAGTTCA AACAGAAATA TGACTATTTC
    CGGAAGAAGT TGAAGAAACC TGATGATATA CCAAGCAGGT TTGAGATGAA GCTGCACAGA
    AATAATATTT TTGAGGAGTC CTACAGAAGA ATCATGTCTG TGAAAAGACC TGATGTACTG
    AAGGCCAGGC TCTGGATTGA ATTTGAGTCA GAAAAAGGAC TTGACTATGG AGGTGTGGCT
    AGAGAATGGT TTTTTCTCTT GTCCAAGGAG ATGTTCAACC CATATTATGG TCTCTTTGAA
    TATTCAGCAA CGGACAACTA TACACTTCAG ATTAATCCTA ATTCAGGTCT TTGTAACGAA
    GATCATCTTT CATATTTCAT GTTTATTGGA CGAGTTGCTG GCCTGGCAGT GTATCATGGG
    AAACTATTGG ATGGCTTCTT CATCAGACCT TTTTACAAGA TGATGCTGGG GAAGCCAATA
    ATTCTGAAAG ATATGGAATC AGTGGACAGT GAATACTACA ACTCTTTGAA GTGGATCCTG
    GAAAATGACC CTACAGAGCT GGATCTCATG TTTTGCATTG ATGAGGAAAA CTTCGGACAG
    ACATATCAAG TAGACCTGAA GCCAAATGGG TCTGAAATCA TGGTAACAAA TGAAAACAAA
    AGAGAATATA TTGATCTGGT CATCCAGTGG CGGTTTGTAA ACAGAGTTCA AAAACAAATG
    AATGCTTTTT TGGAGGGATT CACAGAACTT CTTCCTATTG ATCTGATTAA AATTTTTGAT
    GAAAACGAAC TAGAGTTACT GATGTGTGGC CTTGGCGATG TTGACGTTAA TGACTGGAGA
    CAACATACAG TCTACAAAAA TGGCTACTGC CCAAATCATC CTGTTATCCA GTGGTTCTGG
    AAGGCTGTTT TACTGATGGA TGCAGAAAAA CGTATTCGGT TACTGCAGTT TGTTACTGGC
    ACATCACGTG TGCCTATGAA TGGATTTGCT GAACTTTATG GTTCAAATGG TCCTCAGCTG
    TTTACAATAG AGCAATGGGG AACTCCTGAT AAACTGCCCA GAGCTCACAC ATGCTTTAAT
    CGCTTAGACT TACCTCTTTA TGAATCTTTT GACAACCTAC AAGAGAAGCT ACTTATGGCA
    GTTGAAAATG CTGAAGGATT TGAAGGAGTG GATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025000322.1
    CDS3347..6202
    Translation

    Target ORF information:

    RefSeq Version XM_025144554.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus E3 ubiquitin-protein ligase NEDD4-like (LOC107055431), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025144554.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCGACTG CGGCGGTGGA GCCTGTGTAC GGGCTGGCGG AAGAGGAGGA GGAGTCTCGT 
    ATCCTCAGGG TGAAGGTGGT CTCTGGCATT GATCTGGCAA AAAAAGACAT CTTTGGAGCC
    AGTGATCCCT ACGTCAAACT TTCTTTGTAT GTAGCAGATG AGAACAGAGA ACTTTCTCTG
    GTGCAGACAA AAACTATTAA AAAGACACTG AATCCAAAAT GGAATGAGGA GTTTTATTTT
    AGAGTAAATC CAACCAATCA TCGCCTCCTG TTTGAAGTGT TTGATGAGAA CAGATTGACT
    AGAGATGACT TCCTGGGTCA GGTGGATTTG CCTCTTAGTC ACCTTCCGAC TGAAGACCCA
    ACCATGGAAA GGCCATACAC GTTTAAGGAT TTCCTTCTCA GACCAAGAAG CCACAAATCT
    CACGTAAAGG GTTTCTTGAG ATTGAAGATG GCTTATATGC CTAAAAATGG TGGTCAAGAG
    GAAGAAAATA GCAATCAAAG GAACGAGTCT GAGCATGAAT GGGATGTGAC TGATTCCAGT
    GACTCTGCAT CTCAGTGTCA GGAGGTGTTA CCACCTCCTC CACTGCCTCC TGGATGGGAA
    GAAAAGGTTG ACAACCTGGG GCGGACATAC TATGTCAACC ACAACAACAG AACTACTCAA
    TGGCATCGAC CAAGTTTAAT TGATGTGGGA TCCGATTCAG AGAACAACAG ACAAATAAAC
    TATGAAGCAG CCCATAGGCG GTTTCGCTCT CGGAGACACA TTAGTGAGGA TTTGGAACCA
    GAACCTATGG AGACTGGAGA CATTCTTGAG CCTTGGGAAG CCATTTCAGA GGAAGCAAGT
    ACAAGTGGAG ATTCTTTAAT TTTATCATTG CCACCACCTC CTGCATCTCC TGTATCCCAT
    GCCAGTCCTC AGGAGCTATC TGAGAAACTG AGCAGTAGAT TTCAGATCAC TAATGAGGAA
    CAGCTGAGTT CTTTGATTCA AAGAGATCCT TCTTCAAGAT TGAGATCTTG CAGTGTAACA
    GATACAGTCG CTGAACAGTC TCGCTTATCT TTGCCTTCAG TGGCCTATGT CCACACTACA
    CCAGGCTTAC CTTCAGGCTG GGAAGAAAGA AAGGATGCAA AGGGCCGTAC TTACTATGTC
    AATCACAACA ATCGAACCAC AACCTGGACA CGGCCCATTA TACAGTTTAC AGAAGATGGC
    ATGGTTGGGT CAGCAGCAAA CAGCAACAGT CATTTAAGTG AACCTCAAAT CAGACGGCCT
    CGCAGCCTCA GTTCACCTAC AGTAACCTTA TCTGCTCCAC TTGAGGGTAT GAAGGACTCT
    CCAGTGCGCA GAGCAGTGAA GGACATACTA TCCAACCCAC AGTCTCTACA GCCCTCACCC
    TACAGCTCTC CTAAACCACA GAACAAAGTT GCACAAAGCT TCCTTCCTCC TGGCTGGGAG
    ATGCGAATAG CACCCAACAG CAGGCCCTTC TTCATCGATC ATAATACTAA AACTACTACA
    TGGGAAGATC CACGTCTGAA ATTTCCAGTG CATTTGAGAT CAAAAGCTTC TTTAAATCCT
    GATGATTTGG GCCCTCTTCC TCCTGGTTGG GAGGAAAGAA TACATTTGGA TGGAAGAATG
    TTTTATATAG ATCATAATAA TAAAATTACC CAATGGGAAG ATCCCAGACT GCAGAGCCCA
    GCAATTACTG GTCCAGCAGT TCCATATTCT AGGGAGTTCA AACAGAAATA TGACTATTTC
    CGGAAGAAGT TGAAGAAACC TGATGATATA CCAAGCAGGT TTGAGATGAA GCTGCACAGA
    AATAATATTT TTGAGGAGTC CTACAGAAGA ATCATGTCTG TGAAAAGACC TGATGTACTG
    AAGGCCAGGC TCTGGATTGA ATTTGAGTCA GAAAAAGGAC TTGACTATGG AGGTGTGGCT
    AGAGAATGGT TTTTTCTCTT GTCCAAGGAG ATGTTCAACC CATATTATGG TCTCTTTGAA
    TATTCAGCAA CGGACAACTA TACACTTCAG ATTAATCCTA ATTCAGGTCT TTGTAACGAA
    GATCATCTTT CATATTTCAT GTTTATTGGA CGAGTTGCTG GCCTGGCAGT GTATCATGGG
    AAACTATTGG ATGGCTTCTT CATCAGACCT TTTTACAAGA TGATGCTGGG GAAGCCAATA
    ATTCTGAAAG ATATGGAATC AGTGGACAGT GAATACTACA ACTCTTTGAA GTGGATCCTG
    GAAAATGACC CTACAGAGCT GGATCTCATG TTTTGCATTG ATGAGGAAAA CTTCGGACAG
    ACATATCAAG TAGACCTGAA GCCAAATGGG TCTGAAATCA TGGTAACAAA TGAAAACAAA
    AGAGAATATA TTGATCTGGT CATCCAGTGG CGGTTTGTAA ACAGAGTTCA AAAACAAATG
    AATGCTTTTT TGGAGGGATT CACAGAACTT CTTCCTATTG ATCTGATTAA AATTTTTGAT
    GAAAACGAAC TAGAGTTACT GATGTGTGGC CTTGGCGATG TTGACGTTAA TGACTGGAGA
    CAACATACAG TCTACAAAAA TGGCTACTGC CCAAATCATC CTGTTATCCA GTGGTTCTGG
    AAGGCTGTTT TACTGATGGA TGCAGAAAAA CGTATTCGGT TACTGCAGTT TGTTACTGGC
    ACATCACGTG TGCCTATGAA TGGATTTGCT GAACTTTATG GTTCAAATGG TCCTCAGCTG
    TTTACAATAG AGCAATGGGG AACTCCTGAT AAACTGCCCA GAGCTCACAC ATGCTTTAAT
    CGCTTAGACT TACCTCTTTA TGAATCTTTT GACAACCTAC AAGAGAAGCT ACTTATGGCA
    GTTGAAAATG CTGAAGGATT TGAAGGAGTG GATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152897
    Clone ID Related Accession (Same CDS sequence) XM_025144555.1
    Accession Version XM_025144555.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2748bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006126.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACTG CGGCGGTGGA GCCTGTGTAC GGGCTGGCGG AAGAGGAGGA GGAGTCTCGT 
    ATCCTCAGGG TGAAGGTGGT CTCTGGCATT GATCTGGCAA AAAAAGACAT CTTTGGAGCC
    AGTGATCCCT ACGTCAAACT TTCTTTGTAT GTAGCAGATG AGAACAGAGA ACTTTCTCTG
    GTGCAGACAA AAACTATTAA AAAGACACTG AATCCAAAAT GGAATGAGGA GTTTTATTTT
    AGAGTAAATC CAACCAATCA TCGCCTCCTG TTTGAAGTGT TTGATGAGAA CAGATTGACT
    AGAGATGACT TCCTGGGTCA GGTGGATTTG CCTCTTAGTC ACCTTCCGAC TGAAGACCCA
    ACCATGGAAA GGCCATACAC GTTTAAGGAT TTCCTTCTCA GACCAAGAAG CCACAAATCT
    CACGTAAAGG GTTTCTTGAG ATTGAAGATG GCTTATATGC CTAAAAATGG TGGTCAAGAG
    GAAGAAAATA GCAATCAAAG GAACGAGTCT GAGCATGAAT GGGATGTGAC TGATTCCAGT
    GACTCTGCAT CTCAGTGTCA GGAGGTGTTA CCACCTCCTC CACTGCCTCC TGGATGGGAA
    GAAAAGGTTG ACAACCTGGG GCGGACATAC TATGTCAACC ACAACAACAG AACTACTCAA
    TGGCATCGAC CAAGTTTAAT TGATGTGGGA TCCGATTCAG AGAACAACAG ACAAATAAAC
    TATGAAGCAG CCCATAGGCG GTTTCGCTCT CGGAGACACA TTAGTGAGGA TTTGGAACCA
    GAACCTATGG AGACTGGAGA CATTCTTGAG CAAAGAGATC CTTCTTCAAG ATTGAGATCT
    TGCAGTGTAA CAGATACAGT CGCTGAACAG TCTCGCTTAT CTTTGCAGGG AGGCCCATCT
    AGGAAAACTC GTTCTTCAGC TGTCACAGAT GGTGAGGAAA CAATGCCTTC AGTGGCCTAT
    GTCCACACTA CACCAGGCTT ACCTTCAGGC TGGGAAGAAA GAAAGGATGC AAAGGGCCGT
    ACTTACTATG TCAATCACAA CAATCGAACC ACAACCTGGA CACGGCCCAT TATACAGTTT
    ACAGAAGATG GCATGGTTGG GTCAGCAGCA AACAGCAACA GTCATTTAAG TGAACCTCAA
    ATCAGACGGC CTCGCAGCCT CAGTTCACCT ACAGTAACCT TATCTGCTCC ACTTGAGGGT
    ATGAAGGACT CTCCAGTGCG CAGAGCAGTG AAGGACATAC TATCCAACCC ACAGTCTCTA
    CAGCCCTCAC CCTACAGCTC TCCTAAACCA CAGAACAAAG TTGCACAAAG CTTCCTTCCT
    CCTGGCTGGG AGATGCGAAT AGCACCCAAC AGCAGGCCCT TCTTCATCGA TCATAATACT
    AAAACTACTA CATGGGAAGA TCCACGTCTG AAATTTCCAG TGCATTTGAG ATCAAAAGCT
    TCTTTAAATC CTGATGATTT GGGCCCTCTT CCTCCTGGTT GGGAGGAAAG AATACATTTG
    GATGGAAGAA TGTTTTATAT AGATCATAAT AATAAAATTA CCCAATGGGA AGATCCCAGA
    CTGCAGAGCC CAGCAATTAC TGGTCCAGCA GTTCCATATT CTAGGGAGTT CAAACAGAAA
    TATGACTATT TCCGGAAGAA GTTGAAGAAA CCTGATGATA TACCAAGCAG GTTTGAGATG
    AAGCTGCACA GAAATAATAT TTTTGAGGAG TCCTACAGAA GAATCATGTC TGTGAAAAGA
    CCTGATGTAC TGAAGGCCAG GCTCTGGATT GAATTTGAGT CAGAAAAAGG ACTTGACTAT
    GGAGGTGTGG CTAGAGAATG GTTTTTTCTC TTGTCCAAGG AGATGTTCAA CCCATATTAT
    GGTCTCTTTG AATATTCAGC AACGGACAAC TATACACTTC AGATTAATCC TAATTCAGGT
    CTTTGTAACG AAGATCATCT TTCATATTTC ATGTTTATTG GACGAGTTGC TGGCCTGGCA
    GTGTATCATG GGAAACTATT GGATGGCTTC TTCATCAGAC CTTTTTACAA GATGATGCTG
    GGGAAGCCAA TAATTCTGAA AGATATGGAA TCAGTGGACA GTGAATACTA CAACTCTTTG
    AAGTGGATCC TGGAAAATGA CCCTACAGAG CTGGATCTCA TGTTTTGCAT TGATGAGGAA
    AACTTCGGAC AGACATATCA AGTAGACCTG AAGCCAAATG GGTCTGAAAT CATGGTAACA
    AATGAAAACA AAAGAGAATA TATTGATCTG GTCATCCAGT GGCGGTTTGT AAACAGAGTT
    CAAAAACAAA TGAATGCTTT TTTGGAGGGA TTCACAGAAC TTCTTCCTAT TGATCTGATT
    AAAATTTTTG ATGAAAACGA ACTAGAGTTA CTGATGTGTG GCCTTGGCGA TGTTGACGTT
    AATGACTGGA GACAACATAC AGTCTACAAA AATGGCTACT GCCCAAATCA TCCTGTTATC
    CAGTGGTTCT GGAAGGCTGT TTTACTGATG GATGCAGAAA AACGTATTCG GTTACTGCAG
    TTTGTTACTG GCACATCACG TGTGCCTATG AATGGATTTG CTGAACTTTA TGGTTCAAAT
    GGTCCTCAGC TGTTTACAAT AGAGCAATGG GGAACTCCTG ATAAACTGCC CAGAGCTCAC
    ACATGCTTTA ATCGCTTAGA CTTACCTCTT TATGAATCTT TTGACAACCT ACAAGAGAAG
    CTACTTATGG CAGTTGAAAA TGCTGAAGGA TTTGAAGGAG TGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025000323.1
    CDS3347..6094
    Translation

    Target ORF information:

    RefSeq Version XM_025144555.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus E3 ubiquitin-protein ligase NEDD4-like (LOC107055431), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025144555.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACTG CGGCGGTGGA GCCTGTGTAC GGGCTGGCGG AAGAGGAGGA GGAGTCTCGT 
    ATCCTCAGGG TGAAGGTGGT CTCTGGCATT GATCTGGCAA AAAAAGACAT CTTTGGAGCC
    AGTGATCCCT ACGTCAAACT TTCTTTGTAT GTAGCAGATG AGAACAGAGA ACTTTCTCTG
    GTGCAGACAA AAACTATTAA AAAGACACTG AATCCAAAAT GGAATGAGGA GTTTTATTTT
    AGAGTAAATC CAACCAATCA TCGCCTCCTG TTTGAAGTGT TTGATGAGAA CAGATTGACT
    AGAGATGACT TCCTGGGTCA GGTGGATTTG CCTCTTAGTC ACCTTCCGAC TGAAGACCCA
    ACCATGGAAA GGCCATACAC GTTTAAGGAT TTCCTTCTCA GACCAAGAAG CCACAAATCT
    CACGTAAAGG GTTTCTTGAG ATTGAAGATG GCTTATATGC CTAAAAATGG TGGTCAAGAG
    GAAGAAAATA GCAATCAAAG GAACGAGTCT GAGCATGAAT GGGATGTGAC TGATTCCAGT
    GACTCTGCAT CTCAGTGTCA GGAGGTGTTA CCACCTCCTC CACTGCCTCC TGGATGGGAA
    GAAAAGGTTG ACAACCTGGG GCGGACATAC TATGTCAACC ACAACAACAG AACTACTCAA
    TGGCATCGAC CAAGTTTAAT TGATGTGGGA TCCGATTCAG AGAACAACAG ACAAATAAAC
    TATGAAGCAG CCCATAGGCG GTTTCGCTCT CGGAGACACA TTAGTGAGGA TTTGGAACCA
    GAACCTATGG AGACTGGAGA CATTCTTGAG CAAAGAGATC CTTCTTCAAG ATTGAGATCT
    TGCAGTGTAA CAGATACAGT CGCTGAACAG TCTCGCTTAT CTTTGCAGGG AGGCCCATCT
    AGGAAAACTC GTTCTTCAGC TGTCACAGAT GGTGAGGAAA CAATGCCTTC AGTGGCCTAT
    GTCCACACTA CACCAGGCTT ACCTTCAGGC TGGGAAGAAA GAAAGGATGC AAAGGGCCGT
    ACTTACTATG TCAATCACAA CAATCGAACC ACAACCTGGA CACGGCCCAT TATACAGTTT
    ACAGAAGATG GCATGGTTGG GTCAGCAGCA AACAGCAACA GTCATTTAAG TGAACCTCAA
    ATCAGACGGC CTCGCAGCCT CAGTTCACCT ACAGTAACCT TATCTGCTCC ACTTGAGGGT
    ATGAAGGACT CTCCAGTGCG CAGAGCAGTG AAGGACATAC TATCCAACCC ACAGTCTCTA
    CAGCCCTCAC CCTACAGCTC TCCTAAACCA CAGAACAAAG TTGCACAAAG CTTCCTTCCT
    CCTGGCTGGG AGATGCGAAT AGCACCCAAC AGCAGGCCCT TCTTCATCGA TCATAATACT
    AAAACTACTA CATGGGAAGA TCCACGTCTG AAATTTCCAG TGCATTTGAG ATCAAAAGCT
    TCTTTAAATC CTGATGATTT GGGCCCTCTT CCTCCTGGTT GGGAGGAAAG AATACATTTG
    GATGGAAGAA TGTTTTATAT AGATCATAAT AATAAAATTA CCCAATGGGA AGATCCCAGA
    CTGCAGAGCC CAGCAATTAC TGGTCCAGCA GTTCCATATT CTAGGGAGTT CAAACAGAAA
    TATGACTATT TCCGGAAGAA GTTGAAGAAA CCTGATGATA TACCAAGCAG GTTTGAGATG
    AAGCTGCACA GAAATAATAT TTTTGAGGAG TCCTACAGAA GAATCATGTC TGTGAAAAGA
    CCTGATGTAC TGAAGGCCAG GCTCTGGATT GAATTTGAGT CAGAAAAAGG ACTTGACTAT
    GGAGGTGTGG CTAGAGAATG GTTTTTTCTC TTGTCCAAGG AGATGTTCAA CCCATATTAT
    GGTCTCTTTG AATATTCAGC AACGGACAAC TATACACTTC AGATTAATCC TAATTCAGGT
    CTTTGTAACG AAGATCATCT TTCATATTTC ATGTTTATTG GACGAGTTGC TGGCCTGGCA
    GTGTATCATG GGAAACTATT GGATGGCTTC TTCATCAGAC CTTTTTACAA GATGATGCTG
    GGGAAGCCAA TAATTCTGAA AGATATGGAA TCAGTGGACA GTGAATACTA CAACTCTTTG
    AAGTGGATCC TGGAAAATGA CCCTACAGAG CTGGATCTCA TGTTTTGCAT TGATGAGGAA
    AACTTCGGAC AGACATATCA AGTAGACCTG AAGCCAAATG GGTCTGAAAT CATGGTAACA
    AATGAAAACA AAAGAGAATA TATTGATCTG GTCATCCAGT GGCGGTTTGT AAACAGAGTT
    CAAAAACAAA TGAATGCTTT TTTGGAGGGA TTCACAGAAC TTCTTCCTAT TGATCTGATT
    AAAATTTTTG ATGAAAACGA ACTAGAGTTA CTGATGTGTG GCCTTGGCGA TGTTGACGTT
    AATGACTGGA GACAACATAC AGTCTACAAA AATGGCTACT GCCCAAATCA TCCTGTTATC
    CAGTGGTTCT GGAAGGCTGT TTTACTGATG GATGCAGAAA AACGTATTCG GTTACTGCAG
    TTTGTTACTG GCACATCACG TGTGCCTATG AATGGATTTG CTGAACTTTA TGGTTCAAAT
    GGTCCTCAGC TGTTTACAAT AGAGCAATGG GGAACTCCTG ATAAACTGCC CAGAGCTCAC
    ACATGCTTTA ATCGCTTAGA CTTACCTCTT TATGAATCTT TTGACAACCT ACAAGAGAAG
    CTACTTATGG CAGTTGAAAA TGCTGAAGGA TTTGAAGGAG TGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152898
    Clone ID Related Accession (Same CDS sequence) XM_025144556.1
    Accession Version XM_025144556.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2688bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006126.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGCGACTG CGGCGGTGGA GCCTGTGTAC GGGCTGGCGG AAGAGGAGGA GGAGTCTCGT 
    ATCCTCAGGG TGAAGGTGGT CTCTGGCATT GATCTGGCAA AAAAAGACAT CTTTGGAGCC
    AGTGATCCCT ACGTCAAACT TTCTTTGTAT GTAGCAGATG AGAACAGAGA ACTTTCTCTG
    GTGCAGACAA AAACTATTAA AAAGACACTG AATCCAAAAT GGAATGAGGA GTTTTATTTT
    AGAGTAAATC CAACCAATCA TCGCCTCCTG TTTGAAGTGT TTGATGAGAA CAGATTGACT
    AGAGATGACT TCCTGGGTCA GGTGGATTTG CCTCTTAGTC ACCTTCCGAC TGAAGACCCA
    ACCATGGAAA GGCCATACAC GTTTAAGGAT TTCCTTCTCA GACCAAGAAG CCACAAATCT
    CACGTAAAGG GTTTCTTGAG ATTGAAGATG GCTTATATGC CTAAAAATGG TGGTCAAGAG
    GAAGAAAATA GCAATCAAAG GAACGAGTCT GAGCATGAAT GGGATGTGAC TGATTCCAGT
    GACTCTGCAT CTCAGTGTCA GGAGGTGTTA CCACCTCCTC CACTGCCTCC TGGATGGGAA
    GAAAAGGTTG ACAACCTGGG GCGGACATAC TATGTCAACC ACAACAACAG AACTACTCAA
    TGGCATCGAC CAAGTTTAAT TGATGTGGGA TCCGATTCAG AGAACAACAG ACAAATAAAC
    TATGAAGCAG CCCATAGGCG GTTTCGCTCT CGGAGACACA TTAGTGAGGA TTTGGAACCA
    GAACCTATGG AGACTGGAGA CATTCTTGAG CAAAGAGATC CTTCTTCAAG ATTGAGATCT
    TGCAGTGTAA CAGATACAGT CGCTGAACAG TCTCGCTTAT CTTTGCCTTC AGTGGCCTAT
    GTCCACACTA CACCAGGCTT ACCTTCAGGC TGGGAAGAAA GAAAGGATGC AAAGGGCCGT
    ACTTACTATG TCAATCACAA CAATCGAACC ACAACCTGGA CACGGCCCAT TATACAGTTT
    ACAGAAGATG GCATGGTTGG GTCAGCAGCA AACAGCAACA GTCATTTAAG TGAACCTCAA
    ATCAGACGGC CTCGCAGCCT CAGTTCACCT ACAGTAACCT TATCTGCTCC ACTTGAGGGT
    ATGAAGGACT CTCCAGTGCG CAGAGCAGTG AAGGACATAC TATCCAACCC ACAGTCTCTA
    CAGCCCTCAC CCTACAGCTC TCCTAAACCA CAGAACAAAG TTGCACAAAG CTTCCTTCCT
    CCTGGCTGGG AGATGCGAAT AGCACCCAAC AGCAGGCCCT TCTTCATCGA TCATAATACT
    AAAACTACTA CATGGGAAGA TCCACGTCTG AAATTTCCAG TGCATTTGAG ATCAAAAGCT
    TCTTTAAATC CTGATGATTT GGGCCCTCTT CCTCCTGGTT GGGAGGAAAG AATACATTTG
    GATGGAAGAA TGTTTTATAT AGATCATAAT AATAAAATTA CCCAATGGGA AGATCCCAGA
    CTGCAGAGCC CAGCAATTAC TGGTCCAGCA GTTCCATATT CTAGGGAGTT CAAACAGAAA
    TATGACTATT TCCGGAAGAA GTTGAAGAAA CCTGATGATA TACCAAGCAG GTTTGAGATG
    AAGCTGCACA GAAATAATAT TTTTGAGGAG TCCTACAGAA GAATCATGTC TGTGAAAAGA
    CCTGATGTAC TGAAGGCCAG GCTCTGGATT GAATTTGAGT CAGAAAAAGG ACTTGACTAT
    GGAGGTGTGG CTAGAGAATG GTTTTTTCTC TTGTCCAAGG AGATGTTCAA CCCATATTAT
    GGTCTCTTTG AATATTCAGC AACGGACAAC TATACACTTC AGATTAATCC TAATTCAGGT
    CTTTGTAACG AAGATCATCT TTCATATTTC ATGTTTATTG GACGAGTTGC TGGCCTGGCA
    GTGTATCATG GGAAACTATT GGATGGCTTC TTCATCAGAC CTTTTTACAA GATGATGCTG
    GGGAAGCCAA TAATTCTGAA AGATATGGAA TCAGTGGACA GTGAATACTA CAACTCTTTG
    AAGTGGATCC TGGAAAATGA CCCTACAGAG CTGGATCTCA TGTTTTGCAT TGATGAGGAA
    AACTTCGGAC AGACATATCA AGTAGACCTG AAGCCAAATG GGTCTGAAAT CATGGTAACA
    AATGAAAACA AAAGAGAATA TATTGATCTG GTCATCCAGT GGCGGTTTGT AAACAGAGTT
    CAAAAACAAA TGAATGCTTT TTTGGAGGGA TTCACAGAAC TTCTTCCTAT TGATCTGATT
    AAAATTTTTG ATGAAAACGA ACTAGAGTTA CTGATGTGTG GCCTTGGCGA TGTTGACGTT
    AATGACTGGA GACAACATAC AGTCTACAAA AATGGCTACT GCCCAAATCA TCCTGTTATC
    CAGTGGTTCT GGAAGGCTGT TTTACTGATG GATGCAGAAA AACGTATTCG GTTACTGCAG
    TTTGTTACTG GCACATCACG TGTGCCTATG AATGGATTTG CTGAACTTTA TGGTTCAAAT
    GGTCCTCAGC TGTTTACAAT AGAGCAATGG GGAACTCCTG ATAAACTGCC CAGAGCTCAC
    ACATGCTTTA ATCGCTTAGA CTTACCTCTT TATGAATCTT TTGACAACCT ACAAGAGAAG
    CTACTTATGG CAGTTGAAAA TGCTGAAGGA TTTGAAGGAG TGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025000324.1
    CDS3347..6034
    Translation

    Target ORF information:

    RefSeq Version XM_025144556.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus E3 ubiquitin-protein ligase NEDD4-like (LOC107055431), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025144556.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGCGACTG CGGCGGTGGA GCCTGTGTAC GGGCTGGCGG AAGAGGAGGA GGAGTCTCGT 
    ATCCTCAGGG TGAAGGTGGT CTCTGGCATT GATCTGGCAA AAAAAGACAT CTTTGGAGCC
    AGTGATCCCT ACGTCAAACT TTCTTTGTAT GTAGCAGATG AGAACAGAGA ACTTTCTCTG
    GTGCAGACAA AAACTATTAA AAAGACACTG AATCCAAAAT GGAATGAGGA GTTTTATTTT
    AGAGTAAATC CAACCAATCA TCGCCTCCTG TTTGAAGTGT TTGATGAGAA CAGATTGACT
    AGAGATGACT TCCTGGGTCA GGTGGATTTG CCTCTTAGTC ACCTTCCGAC TGAAGACCCA
    ACCATGGAAA GGCCATACAC GTTTAAGGAT TTCCTTCTCA GACCAAGAAG CCACAAATCT
    CACGTAAAGG GTTTCTTGAG ATTGAAGATG GCTTATATGC CTAAAAATGG TGGTCAAGAG
    GAAGAAAATA GCAATCAAAG GAACGAGTCT GAGCATGAAT GGGATGTGAC TGATTCCAGT
    GACTCTGCAT CTCAGTGTCA GGAGGTGTTA CCACCTCCTC CACTGCCTCC TGGATGGGAA
    GAAAAGGTTG ACAACCTGGG GCGGACATAC TATGTCAACC ACAACAACAG AACTACTCAA
    TGGCATCGAC CAAGTTTAAT TGATGTGGGA TCCGATTCAG AGAACAACAG ACAAATAAAC
    TATGAAGCAG CCCATAGGCG GTTTCGCTCT CGGAGACACA TTAGTGAGGA TTTGGAACCA
    GAACCTATGG AGACTGGAGA CATTCTTGAG CAAAGAGATC CTTCTTCAAG ATTGAGATCT
    TGCAGTGTAA CAGATACAGT CGCTGAACAG TCTCGCTTAT CTTTGCCTTC AGTGGCCTAT
    GTCCACACTA CACCAGGCTT ACCTTCAGGC TGGGAAGAAA GAAAGGATGC AAAGGGCCGT
    ACTTACTATG TCAATCACAA CAATCGAACC ACAACCTGGA CACGGCCCAT TATACAGTTT
    ACAGAAGATG GCATGGTTGG GTCAGCAGCA AACAGCAACA GTCATTTAAG TGAACCTCAA
    ATCAGACGGC CTCGCAGCCT CAGTTCACCT ACAGTAACCT TATCTGCTCC ACTTGAGGGT
    ATGAAGGACT CTCCAGTGCG CAGAGCAGTG AAGGACATAC TATCCAACCC ACAGTCTCTA
    CAGCCCTCAC CCTACAGCTC TCCTAAACCA CAGAACAAAG TTGCACAAAG CTTCCTTCCT
    CCTGGCTGGG AGATGCGAAT AGCACCCAAC AGCAGGCCCT TCTTCATCGA TCATAATACT
    AAAACTACTA CATGGGAAGA TCCACGTCTG AAATTTCCAG TGCATTTGAG ATCAAAAGCT
    TCTTTAAATC CTGATGATTT GGGCCCTCTT CCTCCTGGTT GGGAGGAAAG AATACATTTG
    GATGGAAGAA TGTTTTATAT AGATCATAAT AATAAAATTA CCCAATGGGA AGATCCCAGA
    CTGCAGAGCC CAGCAATTAC TGGTCCAGCA GTTCCATATT CTAGGGAGTT CAAACAGAAA
    TATGACTATT TCCGGAAGAA GTTGAAGAAA CCTGATGATA TACCAAGCAG GTTTGAGATG
    AAGCTGCACA GAAATAATAT TTTTGAGGAG TCCTACAGAA GAATCATGTC TGTGAAAAGA
    CCTGATGTAC TGAAGGCCAG GCTCTGGATT GAATTTGAGT CAGAAAAAGG ACTTGACTAT
    GGAGGTGTGG CTAGAGAATG GTTTTTTCTC TTGTCCAAGG AGATGTTCAA CCCATATTAT
    GGTCTCTTTG AATATTCAGC AACGGACAAC TATACACTTC AGATTAATCC TAATTCAGGT
    CTTTGTAACG AAGATCATCT TTCATATTTC ATGTTTATTG GACGAGTTGC TGGCCTGGCA
    GTGTATCATG GGAAACTATT GGATGGCTTC TTCATCAGAC CTTTTTACAA GATGATGCTG
    GGGAAGCCAA TAATTCTGAA AGATATGGAA TCAGTGGACA GTGAATACTA CAACTCTTTG
    AAGTGGATCC TGGAAAATGA CCCTACAGAG CTGGATCTCA TGTTTTGCAT TGATGAGGAA
    AACTTCGGAC AGACATATCA AGTAGACCTG AAGCCAAATG GGTCTGAAAT CATGGTAACA
    AATGAAAACA AAAGAGAATA TATTGATCTG GTCATCCAGT GGCGGTTTGT AAACAGAGTT
    CAAAAACAAA TGAATGCTTT TTTGGAGGGA TTCACAGAAC TTCTTCCTAT TGATCTGATT
    AAAATTTTTG ATGAAAACGA ACTAGAGTTA CTGATGTGTG GCCTTGGCGA TGTTGACGTT
    AATGACTGGA GACAACATAC AGTCTACAAA AATGGCTACT GCCCAAATCA TCCTGTTATC
    CAGTGGTTCT GGAAGGCTGT TTTACTGATG GATGCAGAAA AACGTATTCG GTTACTGCAG
    TTTGTTACTG GCACATCACG TGTGCCTATG AATGGATTTG CTGAACTTTA TGGTTCAAAT
    GGTCCTCAGC TGTTTACAAT AGAGCAATGG GGAACTCCTG ATAAACTGCC CAGAGCTCAC
    ACATGCTTTA ATCGCTTAGA CTTACCTCTT TATGAATCTT TTGACAACCT ACAAGAGAAG
    CTACTTATGG CAGTTGAAAA TGCTGAAGGA TTTGAAGGAG TGGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152899
    Clone ID Related Accession (Same CDS sequence) XM_025144557.1
    Accession Version XM_025144557.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2613bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase NEDD4-like isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006126.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCGACTG CGGCGGTGGA GCCTGTGTAC GGGCTGGCGG AAGAGGAGGA GGAGTCTCGT 
    ATCCTCAGGG TGAAGGTGGT CTCTGGCATT GATCTGGCAA AAAAAGACAT CTTTGGAGCC
    AGTGATCCCT ACGTCAAACT TTCTTTGTAT GTAGCAGATG AGAACAGAGA ACTTTCTCTG
    GTGCAGACAA AAACTATTAA AAAGACACTG AATCCAAAAT GGAATGAGGA GTTTTATTTT
    AGAGTAAATC CAACCAATCA TCGCCTCCTG TTTGAAGTGT TTGATGAGAA CAGATTGACT
    AGAGATGACT TCCTGGGTCA GGTGGATTTG CCTCTTAGTC ACCTTCCGAC TGAAGACCCA
    ACCATGGAAA GGCCATACAC GTTTAAGGAT TTCCTTCTCA GACCAAGAAG CCACAAATCT
    CACGTAAAGG GTTTCTTGAG ATTGAAGATG GCTTATATGC CTAAAAATGG TGGTCAAGAG
    GAAGAAAATA GCAATCAAAG GAACGAGTCT GAGCATGAAT GGGATGTGAC TGATTCCAGT
    GACTCTGCAT CTCAGTGTCA GGAGGTGTTA CCACCTCCTC CACTGCCTCC TGGATGGGAA
    GAAAAGGTTG ACAACCTGGG GCGGACATAC TATGTCAACC ACAACAACAG AACTACTCAA
    TGGCATCGAC CAAGTTTAAT TGATGTGGGA TCCGATTCAG AGAACAACAG ACAAATAAAC
    TATGAAGCAG CCCATAGGCG GTTTCGCTCT CGGAGACACA TTAGTGAGGA TTTGGAACCA
    GAACCTATGG AGACTGGAGA CATTCTTGAG CCTTCAGTGG CCTATGTCCA CACTACACCA
    GGCTTACCTT CAGGCTGGGA AGAAAGAAAG GATGCAAAGG GCCGTACTTA CTATGTCAAT
    CACAACAATC GAACCACAAC CTGGACACGG CCCATTATAC AGTTTACAGA AGATGGCATG
    GTTGGGTCAG CAGCAAACAG CAACAGTCAT TTAAGTGAAC CTCAAATCAG ACGGCCTCGC
    AGCCTCAGTT CACCTACAGT AACCTTATCT GCTCCACTTG AGGGTATGAA GGACTCTCCA
    GTGCGCAGAG CAGTGAAGGA CATACTATCC AACCCACAGT CTCTACAGCC CTCACCCTAC
    AGCTCTCCTA AACCACAGAA CAAAGTTGCA CAAAGCTTCC TTCCTCCTGG CTGGGAGATG
    CGAATAGCAC CCAACAGCAG GCCCTTCTTC ATCGATCATA ATACTAAAAC TACTACATGG
    GAAGATCCAC GTCTGAAATT TCCAGTGCAT TTGAGATCAA AAGCTTCTTT AAATCCTGAT
    GATTTGGGCC CTCTTCCTCC TGGTTGGGAG GAAAGAATAC ATTTGGATGG AAGAATGTTT
    TATATAGATC ATAATAATAA AATTACCCAA TGGGAAGATC CCAGACTGCA GAGCCCAGCA
    ATTACTGGTC CAGCAGTTCC ATATTCTAGG GAGTTCAAAC AGAAATATGA CTATTTCCGG
    AAGAAGTTGA AGAAACCTGA TGATATACCA AGCAGGTTTG AGATGAAGCT GCACAGAAAT
    AATATTTTTG AGGAGTCCTA CAGAAGAATC ATGTCTGTGA AAAGACCTGA TGTACTGAAG
    GCCAGGCTCT GGATTGAATT TGAGTCAGAA AAAGGACTTG ACTATGGAGG TGTGGCTAGA
    GAATGGTTTT TTCTCTTGTC CAAGGAGATG TTCAACCCAT ATTATGGTCT CTTTGAATAT
    TCAGCAACGG ACAACTATAC ACTTCAGATT AATCCTAATT CAGGTCTTTG TAACGAAGAT
    CATCTTTCAT ATTTCATGTT TATTGGACGA GTTGCTGGCC TGGCAGTGTA TCATGGGAAA
    CTATTGGATG GCTTCTTCAT CAGACCTTTT TACAAGATGA TGCTGGGGAA GCCAATAATT
    CTGAAAGATA TGGAATCAGT GGACAGTGAA TACTACAACT CTTTGAAGTG GATCCTGGAA
    AATGACCCTA CAGAGCTGGA TCTCATGTTT TGCATTGATG AGGAAAACTT CGGACAGACA
    TATCAAGTAG ACCTGAAGCC AAATGGGTCT GAAATCATGG TAACAAATGA AAACAAAAGA
    GAATATATTG ATCTGGTCAT CCAGTGGCGG TTTGTAAACA GAGTTCAAAA ACAAATGAAT
    GCTTTTTTGG AGGGATTCAC AGAACTTCTT CCTATTGATC TGATTAAAAT TTTTGATGAA
    AACGAACTAG AGTTACTGAT GTGTGGCCTT GGCGATGTTG ACGTTAATGA CTGGAGACAA
    CATACAGTCT ACAAAAATGG CTACTGCCCA AATCATCCTG TTATCCAGTG GTTCTGGAAG
    GCTGTTTTAC TGATGGATGC AGAAAAACGT ATTCGGTTAC TGCAGTTTGT TACTGGCACA
    TCACGTGTGC CTATGAATGG ATTTGCTGAA CTTTATGGTT CAAATGGTCC TCAGCTGTTT
    ACAATAGAGC AATGGGGAAC TCCTGATAAA CTGCCCAGAG CTCACACATG CTTTAATCGC
    TTAGACTTAC CTCTTTATGA ATCTTTTGAC AACCTACAAG AGAAGCTACT TATGGCAGTT
    GAAAATGCTG AAGGATTTGA AGGAGTGGAT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025000325.1
    CDS3346..5958
    Translation

    Target ORF information:

    RefSeq Version XM_025144557.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus E3 ubiquitin-protein ligase NEDD4-like (LOC107055431), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025144557.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCGACTG CGGCGGTGGA GCCTGTGTAC GGGCTGGCGG AAGAGGAGGA GGAGTCTCGT 
    ATCCTCAGGG TGAAGGTGGT CTCTGGCATT GATCTGGCAA AAAAAGACAT CTTTGGAGCC
    AGTGATCCCT ACGTCAAACT TTCTTTGTAT GTAGCAGATG AGAACAGAGA ACTTTCTCTG
    GTGCAGACAA AAACTATTAA AAAGACACTG AATCCAAAAT GGAATGAGGA GTTTTATTTT
    AGAGTAAATC CAACCAATCA TCGCCTCCTG TTTGAAGTGT TTGATGAGAA CAGATTGACT
    AGAGATGACT TCCTGGGTCA GGTGGATTTG CCTCTTAGTC ACCTTCCGAC TGAAGACCCA
    ACCATGGAAA GGCCATACAC GTTTAAGGAT TTCCTTCTCA GACCAAGAAG CCACAAATCT
    CACGTAAAGG GTTTCTTGAG ATTGAAGATG GCTTATATGC CTAAAAATGG TGGTCAAGAG
    GAAGAAAATA GCAATCAAAG GAACGAGTCT GAGCATGAAT GGGATGTGAC TGATTCCAGT
    GACTCTGCAT CTCAGTGTCA GGAGGTGTTA CCACCTCCTC CACTGCCTCC TGGATGGGAA
    GAAAAGGTTG ACAACCTGGG GCGGACATAC TATGTCAACC ACAACAACAG AACTACTCAA
    TGGCATCGAC CAAGTTTAAT TGATGTGGGA TCCGATTCAG AGAACAACAG ACAAATAAAC
    TATGAAGCAG CCCATAGGCG GTTTCGCTCT CGGAGACACA TTAGTGAGGA TTTGGAACCA
    GAACCTATGG AGACTGGAGA CATTCTTGAG CCTTCAGTGG CCTATGTCCA CACTACACCA
    GGCTTACCTT CAGGCTGGGA AGAAAGAAAG GATGCAAAGG GCCGTACTTA CTATGTCAAT
    CACAACAATC GAACCACAAC CTGGACACGG CCCATTATAC AGTTTACAGA AGATGGCATG
    GTTGGGTCAG CAGCAAACAG CAACAGTCAT TTAAGTGAAC CTCAAATCAG ACGGCCTCGC
    AGCCTCAGTT CACCTACAGT AACCTTATCT GCTCCACTTG AGGGTATGAA GGACTCTCCA
    GTGCGCAGAG CAGTGAAGGA CATACTATCC AACCCACAGT CTCTACAGCC CTCACCCTAC
    AGCTCTCCTA AACCACAGAA CAAAGTTGCA CAAAGCTTCC TTCCTCCTGG CTGGGAGATG
    CGAATAGCAC CCAACAGCAG GCCCTTCTTC ATCGATCATA ATACTAAAAC TACTACATGG
    GAAGATCCAC GTCTGAAATT TCCAGTGCAT TTGAGATCAA AAGCTTCTTT AAATCCTGAT
    GATTTGGGCC CTCTTCCTCC TGGTTGGGAG GAAAGAATAC ATTTGGATGG AAGAATGTTT
    TATATAGATC ATAATAATAA AATTACCCAA TGGGAAGATC CCAGACTGCA GAGCCCAGCA
    ATTACTGGTC CAGCAGTTCC ATATTCTAGG GAGTTCAAAC AGAAATATGA CTATTTCCGG
    AAGAAGTTGA AGAAACCTGA TGATATACCA AGCAGGTTTG AGATGAAGCT GCACAGAAAT
    AATATTTTTG AGGAGTCCTA CAGAAGAATC ATGTCTGTGA AAAGACCTGA TGTACTGAAG
    GCCAGGCTCT GGATTGAATT TGAGTCAGAA AAAGGACTTG ACTATGGAGG TGTGGCTAGA
    GAATGGTTTT TTCTCTTGTC CAAGGAGATG TTCAACCCAT ATTATGGTCT CTTTGAATAT
    TCAGCAACGG ACAACTATAC ACTTCAGATT AATCCTAATT CAGGTCTTTG TAACGAAGAT
    CATCTTTCAT ATTTCATGTT TATTGGACGA GTTGCTGGCC TGGCAGTGTA TCATGGGAAA
    CTATTGGATG GCTTCTTCAT CAGACCTTTT TACAAGATGA TGCTGGGGAA GCCAATAATT
    CTGAAAGATA TGGAATCAGT GGACAGTGAA TACTACAACT CTTTGAAGTG GATCCTGGAA
    AATGACCCTA CAGAGCTGGA TCTCATGTTT TGCATTGATG AGGAAAACTT CGGACAGACA
    TATCAAGTAG ACCTGAAGCC AAATGGGTCT GAAATCATGG TAACAAATGA AAACAAAAGA
    GAATATATTG ATCTGGTCAT CCAGTGGCGG TTTGTAAACA GAGTTCAAAA ACAAATGAAT
    GCTTTTTTGG AGGGATTCAC AGAACTTCTT CCTATTGATC TGATTAAAAT TTTTGATGAA
    AACGAACTAG AGTTACTGAT GTGTGGCCTT GGCGATGTTG ACGTTAATGA CTGGAGACAA
    CATACAGTCT ACAAAAATGG CTACTGCCCA AATCATCCTG TTATCCAGTG GTTCTGGAAG
    GCTGTTTTAC TGATGGATGC AGAAAAACGT ATTCGGTTAC TGCAGTTTGT TACTGGCACA
    TCACGTGTGC CTATGAATGG ATTTGCTGAA CTTTATGGTT CAAATGGTCC TCAGCTGTTT
    ACAATAGAGC AATGGGGAAC TCCTGATAAA CTGCCCAGAG CTCACACATG CTTTAATCGC
    TTAGACTTAC CTCTTTATGA ATCTTTTGAC AACCTACAAG AGAAGCTACT TATGGCAGTT
    GAAAATGCTG AAGGATTTGA AGGAGTGGAT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa47637
    Clone ID Related Accession (Same CDS sequence) XM_015300422.1 , XM_015300423.1
    Accession Version XM_015300422.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1992bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase NEDD4-like
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456079.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAAAGGC CATACACGTT TAAGGATTTC CTTCTCAGAC CAAGAAGCCA CAAATCTCAC 
    GTAAAGGGTT TCTTGAGATT GAAGATGGCT TATATGCCTA AAAATGGTGG TCAAGAGGAA
    GAAAATAGCA ATCAAAGGAA CGAGTCTGAG CATGAATGGG ATGTGACTGA TTCCAGTGAC
    TCTGCATCTC AGTGTCAGGA GGTGTTACCA CCTCCTCCAC TGCCTCCTGG ATGGGAAGAA
    AAGGTTGACA ACCTGGGGCG GACATACTAT GTCAACCACA ACAACAGAAC TACTCAATGG
    CATCGACCAA GTTTAATTGA TGTGGGATCC GATTCAGAGA ACAACAGACA AATAAACTAT
    GAAGCAGCCC ATAGGCGGTT TCGCTCTCGG AGACACATTA GTGAGGATTT GGAACCAGAA
    CCTATGGAGA CTGGAGACAT TCTTGAGCCT TGGGAAGCCA TTTCAGAGGA AGCAAGTACA
    AGTGGAGATT CTTTAATTTT ATCATTGCCA CCACCTCCTG CATCTCCTGT ATCCCATGCC
    AGTCCTCAGG AGCTATCTGA GAAACTGAGC AGTAGATTTC AGATCACTAA TGAGGAACAG
    CTGAGTTCTT TGATTCAAAG AGATCCTTCT TCAAGATTGA GATCTTGCAG TGTAACAGAT
    ACAGTCGCTG AACAGTCTCG CTTATCTTTG CAGGGAGGCC CATCTAGGAA AACTCGTTCT
    TCAGCTGTCA CAGATGGTGA GGAAACAATG CCTTCAGTGG CCTATGTCCA CACTACACCA
    GGCTTACCTT CAGGCTGGGA AGAAAGAAAG GATGCAAAGG GCCGTACTTA CTATGTCAAT
    CACAACAATC GAACCACAAC CTGGACACGG CCCATTATAC AGTTTACAGA AGATGGCATG
    GTTGGGTCAG CAGCAAACAG CAACAGTCAT TTAAGTGAAC CTCAAATCAG ACGGCCTCGC
    AGCCTCAGTT CACCTACAGT AACCTTATCT GCTCCACTTG AGGGTATGAA GGACTCTCCA
    GTGCGCAGAG CAGTGAAGGA CATACTATCC AACCCACAGT CTCTACAGCC CTCACCCTAC
    AGCTCTCCTA AACCACAGAA CAAAGTTGCA CAAAGCTTCC TTCCTCCTGG CTGGGAGATG
    CGAATAGCAC CCAACAGCAG GCCCTTCTTC ATCGATCATA ATACTAAAAC TACTACATGG
    GAAGATCCAC GTCTGAAATT TCCAGTGCAT TTGAGATCAA AAGCTTCTTT AAATCCTGAT
    GATTTGGGCC CTCTTCCTCC TGGTTGGGAG GAAAGAATAC ATTTGGATGG AAGAATGTTT
    TATATAGATC ATAATAATAA AATTACCCAA TGGGAAGATC CCAGACTGCA GAGCCCAGCA
    ATTACTGGTC CAGCAGTTCC ATATTCTAGG GAGTTCAAAC AGAAATATGA CTATTTCCGG
    AAGAAGTTGA AGAAACCTGA TGATATACCA AGCAGGTTTG AGATGAAGCT GCACAGAAAT
    AATATTTTTG AGGAGTCCTA CAGAAGAATC ATGTCTGTGA AAAGACCTGA TGTACTGAAG
    GCCAGGCTCT GGATTGAATT TGAGTCAGAA AAAGGACTTG ACTATGGAGG TGTGGCTAGA
    GAATGGTTTT TTCTCTTGTC CAAGGAGATG TTCAACCCAT ATTATGGTCT CTTTGAATAT
    TCAGCAACGG ACAACTATAC ACTTCAGATT AATCCTAATT CAGGTCTTTG TAACGAAGAT
    CATCTTTCAT ATTTCATGTT TATTGGACGA GTTGCTGGCC TGGCAGTGTA TCATGGGAAA
    CTATTGGATG GCTTCTTCAT CAGACCTTTT TACAAGATGA TGCTGGGGAA GCCAATAATT
    CTGAAAGATA TGGAATCAGT GGACAGTGAA TACTACAACT CTTTGAAGTG GATCCTGGAA
    AATGACCCTA CAGAGCTGGA TCTCATGTTT TGCATTGATG AGGAAAACTT CGGACAGGTG
    TGTAAAGGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015155908.1
    CDS312..2303
    Translation

    Target ORF information:

    RefSeq Version XM_015300422.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus E3 ubiquitin-protein ligase NEDD4-like (LOC107055431), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015300422.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAAAGGC CATACACGTT TAAGGATTTC CTTCTCAGAC CAAGAAGCCA CAAATCTCAC 
    GTAAAGGGTT TCTTGAGATT GAAGATGGCT TATATGCCTA AAAATGGTGG TCAAGAGGAA
    GAAAATAGCA ATCAAAGGAA CGAGTCTGAG CATGAATGGG ATGTGACTGA TTCCAGTGAC
    TCTGCATCTC AGTGTCAGGA GGTGTTACCA CCTCCTCCAC TGCCTCCTGG ATGGGAAGAA
    AAGGTTGACA ACCTGGGGCG GACATACTAT GTCAACCACA ACAACAGAAC TACTCAATGG
    CATCGACCAA GTTTAATTGA TGTGGGATCC GATTCAGAGA ACAACAGACA AATAAACTAT
    GAAGCAGCCC ATAGGCGGTT TCGCTCTCGG AGACACATTA GTGAGGATTT GGAACCAGAA
    CCTATGGAGA CTGGAGACAT TCTTGAGCCT TGGGAAGCCA TTTCAGAGGA AGCAAGTACA
    AGTGGAGATT CTTTAATTTT ATCATTGCCA CCACCTCCTG CATCTCCTGT ATCCCATGCC
    AGTCCTCAGG AGCTATCTGA GAAACTGAGC AGTAGATTTC AGATCACTAA TGAGGAACAG
    CTGAGTTCTT TGATTCAAAG AGATCCTTCT TCAAGATTGA GATCTTGCAG TGTAACAGAT
    ACAGTCGCTG AACAGTCTCG CTTATCTTTG CAGGGAGGCC CATCTAGGAA AACTCGTTCT
    TCAGCTGTCA CAGATGGTGA GGAAACAATG CCTTCAGTGG CCTATGTCCA CACTACACCA
    GGCTTACCTT CAGGCTGGGA AGAAAGAAAG GATGCAAAGG GCCGTACTTA CTATGTCAAT
    CACAACAATC GAACCACAAC CTGGACACGG CCCATTATAC AGTTTACAGA AGATGGCATG
    GTTGGGTCAG CAGCAAACAG CAACAGTCAT TTAAGTGAAC CTCAAATCAG ACGGCCTCGC
    AGCCTCAGTT CACCTACAGT AACCTTATCT GCTCCACTTG AGGGTATGAA GGACTCTCCA
    GTGCGCAGAG CAGTGAAGGA CATACTATCC AACCCACAGT CTCTACAGCC CTCACCCTAC
    AGCTCTCCTA AACCACAGAA CAAAGTTGCA CAAAGCTTCC TTCCTCCTGG CTGGGAGATG
    CGAATAGCAC CCAACAGCAG GCCCTTCTTC ATCGATCATA ATACTAAAAC TACTACATGG
    GAAGATCCAC GTCTGAAATT TCCAGTGCAT TTGAGATCAA AAGCTTCTTT AAATCCTGAT
    GATTTGGGCC CTCTTCCTCC TGGTTGGGAG GAAAGAATAC ATTTGGATGG AAGAATGTTT
    TATATAGATC ATAATAATAA AATTACCCAA TGGGAAGATC CCAGACTGCA GAGCCCAGCA
    ATTACTGGTC CAGCAGTTCC ATATTCTAGG GAGTTCAAAC AGAAATATGA CTATTTCCGG
    AAGAAGTTGA AGAAACCTGA TGATATACCA AGCAGGTTTG AGATGAAGCT GCACAGAAAT
    AATATTTTTG AGGAGTCCTA CAGAAGAATC ATGTCTGTGA AAAGACCTGA TGTACTGAAG
    GCCAGGCTCT GGATTGAATT TGAGTCAGAA AAAGGACTTG ACTATGGAGG TGTGGCTAGA
    GAATGGTTTT TTCTCTTGTC CAAGGAGATG TTCAACCCAT ATTATGGTCT CTTTGAATAT
    TCAGCAACGG ACAACTATAC ACTTCAGATT AATCCTAATT CAGGTCTTTG TAACGAAGAT
    CATCTTTCAT ATTTCATGTT TATTGGACGA GTTGCTGGCC TGGCAGTGTA TCATGGGAAA
    CTATTGGATG GCTTCTTCAT CAGACCTTTT TACAAGATGA TGCTGGGGAA GCCAATAATT
    CTGAAAGATA TGGAATCAGT GGACAGTGAA TACTACAACT CTTTGAAGTG GATCCTGGAA
    AATGACCCTA CAGAGCTGGA TCTCATGTTT TGCATTGATG AGGAAAACTT CGGACAGGTG
    TGTAAAGGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa47637
    Clone ID Related Accession (Same CDS sequence) XM_015300422.1 , XM_015300423.1
    Accession Version XM_015300423.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1992bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase NEDD4-like
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456079.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAAAGGC CATACACGTT TAAGGATTTC CTTCTCAGAC CAAGAAGCCA CAAATCTCAC 
    GTAAAGGGTT TCTTGAGATT GAAGATGGCT TATATGCCTA AAAATGGTGG TCAAGAGGAA
    GAAAATAGCA ATCAAAGGAA CGAGTCTGAG CATGAATGGG ATGTGACTGA TTCCAGTGAC
    TCTGCATCTC AGTGTCAGGA GGTGTTACCA CCTCCTCCAC TGCCTCCTGG ATGGGAAGAA
    AAGGTTGACA ACCTGGGGCG GACATACTAT GTCAACCACA ACAACAGAAC TACTCAATGG
    CATCGACCAA GTTTAATTGA TGTGGGATCC GATTCAGAGA ACAACAGACA AATAAACTAT
    GAAGCAGCCC ATAGGCGGTT TCGCTCTCGG AGACACATTA GTGAGGATTT GGAACCAGAA
    CCTATGGAGA CTGGAGACAT TCTTGAGCCT TGGGAAGCCA TTTCAGAGGA AGCAAGTACA
    AGTGGAGATT CTTTAATTTT ATCATTGCCA CCACCTCCTG CATCTCCTGT ATCCCATGCC
    AGTCCTCAGG AGCTATCTGA GAAACTGAGC AGTAGATTTC AGATCACTAA TGAGGAACAG
    CTGAGTTCTT TGATTCAAAG AGATCCTTCT TCAAGATTGA GATCTTGCAG TGTAACAGAT
    ACAGTCGCTG AACAGTCTCG CTTATCTTTG CAGGGAGGCC CATCTAGGAA AACTCGTTCT
    TCAGCTGTCA CAGATGGTGA GGAAACAATG CCTTCAGTGG CCTATGTCCA CACTACACCA
    GGCTTACCTT CAGGCTGGGA AGAAAGAAAG GATGCAAAGG GCCGTACTTA CTATGTCAAT
    CACAACAATC GAACCACAAC CTGGACACGG CCCATTATAC AGTTTACAGA AGATGGCATG
    GTTGGGTCAG CAGCAAACAG CAACAGTCAT TTAAGTGAAC CTCAAATCAG ACGGCCTCGC
    AGCCTCAGTT CACCTACAGT AACCTTATCT GCTCCACTTG AGGGTATGAA GGACTCTCCA
    GTGCGCAGAG CAGTGAAGGA CATACTATCC AACCCACAGT CTCTACAGCC CTCACCCTAC
    AGCTCTCCTA AACCACAGAA CAAAGTTGCA CAAAGCTTCC TTCCTCCTGG CTGGGAGATG
    CGAATAGCAC CCAACAGCAG GCCCTTCTTC ATCGATCATA ATACTAAAAC TACTACATGG
    GAAGATCCAC GTCTGAAATT TCCAGTGCAT TTGAGATCAA AAGCTTCTTT AAATCCTGAT
    GATTTGGGCC CTCTTCCTCC TGGTTGGGAG GAAAGAATAC ATTTGGATGG AAGAATGTTT
    TATATAGATC ATAATAATAA AATTACCCAA TGGGAAGATC CCAGACTGCA GAGCCCAGCA
    ATTACTGGTC CAGCAGTTCC ATATTCTAGG GAGTTCAAAC AGAAATATGA CTATTTCCGG
    AAGAAGTTGA AGAAACCTGA TGATATACCA AGCAGGTTTG AGATGAAGCT GCACAGAAAT
    AATATTTTTG AGGAGTCCTA CAGAAGAATC ATGTCTGTGA AAAGACCTGA TGTACTGAAG
    GCCAGGCTCT GGATTGAATT TGAGTCAGAA AAAGGACTTG ACTATGGAGG TGTGGCTAGA
    GAATGGTTTT TTCTCTTGTC CAAGGAGATG TTCAACCCAT ATTATGGTCT CTTTGAATAT
    TCAGCAACGG ACAACTATAC ACTTCAGATT AATCCTAATT CAGGTCTTTG TAACGAAGAT
    CATCTTTCAT ATTTCATGTT TATTGGACGA GTTGCTGGCC TGGCAGTGTA TCATGGGAAA
    CTATTGGATG GCTTCTTCAT CAGACCTTTT TACAAGATGA TGCTGGGGAA GCCAATAATT
    CTGAAAGATA TGGAATCAGT GGACAGTGAA TACTACAACT CTTTGAAGTG GATCCTGGAA
    AATGACCCTA CAGAGCTGGA TCTCATGTTT TGCATTGATG AGGAAAACTT CGGACAGGTG
    TGTAAAGGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015155909.1
    CDS261..2252
    Translation

    Target ORF information:

    RefSeq Version XM_015300423.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus E3 ubiquitin-protein ligase NEDD4-like (LOC107055431), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015300423.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAAAGGC CATACACGTT TAAGGATTTC CTTCTCAGAC CAAGAAGCCA CAAATCTCAC 
    GTAAAGGGTT TCTTGAGATT GAAGATGGCT TATATGCCTA AAAATGGTGG TCAAGAGGAA
    GAAAATAGCA ATCAAAGGAA CGAGTCTGAG CATGAATGGG ATGTGACTGA TTCCAGTGAC
    TCTGCATCTC AGTGTCAGGA GGTGTTACCA CCTCCTCCAC TGCCTCCTGG ATGGGAAGAA
    AAGGTTGACA ACCTGGGGCG GACATACTAT GTCAACCACA ACAACAGAAC TACTCAATGG
    CATCGACCAA GTTTAATTGA TGTGGGATCC GATTCAGAGA ACAACAGACA AATAAACTAT
    GAAGCAGCCC ATAGGCGGTT TCGCTCTCGG AGACACATTA GTGAGGATTT GGAACCAGAA
    CCTATGGAGA CTGGAGACAT TCTTGAGCCT TGGGAAGCCA TTTCAGAGGA AGCAAGTACA
    AGTGGAGATT CTTTAATTTT ATCATTGCCA CCACCTCCTG CATCTCCTGT ATCCCATGCC
    AGTCCTCAGG AGCTATCTGA GAAACTGAGC AGTAGATTTC AGATCACTAA TGAGGAACAG
    CTGAGTTCTT TGATTCAAAG AGATCCTTCT TCAAGATTGA GATCTTGCAG TGTAACAGAT
    ACAGTCGCTG AACAGTCTCG CTTATCTTTG CAGGGAGGCC CATCTAGGAA AACTCGTTCT
    TCAGCTGTCA CAGATGGTGA GGAAACAATG CCTTCAGTGG CCTATGTCCA CACTACACCA
    GGCTTACCTT CAGGCTGGGA AGAAAGAAAG GATGCAAAGG GCCGTACTTA CTATGTCAAT
    CACAACAATC GAACCACAAC CTGGACACGG CCCATTATAC AGTTTACAGA AGATGGCATG
    GTTGGGTCAG CAGCAAACAG CAACAGTCAT TTAAGTGAAC CTCAAATCAG ACGGCCTCGC
    AGCCTCAGTT CACCTACAGT AACCTTATCT GCTCCACTTG AGGGTATGAA GGACTCTCCA
    GTGCGCAGAG CAGTGAAGGA CATACTATCC AACCCACAGT CTCTACAGCC CTCACCCTAC
    AGCTCTCCTA AACCACAGAA CAAAGTTGCA CAAAGCTTCC TTCCTCCTGG CTGGGAGATG
    CGAATAGCAC CCAACAGCAG GCCCTTCTTC ATCGATCATA ATACTAAAAC TACTACATGG
    GAAGATCCAC GTCTGAAATT TCCAGTGCAT TTGAGATCAA AAGCTTCTTT AAATCCTGAT
    GATTTGGGCC CTCTTCCTCC TGGTTGGGAG GAAAGAATAC ATTTGGATGG AAGAATGTTT
    TATATAGATC ATAATAATAA AATTACCCAA TGGGAAGATC CCAGACTGCA GAGCCCAGCA
    ATTACTGGTC CAGCAGTTCC ATATTCTAGG GAGTTCAAAC AGAAATATGA CTATTTCCGG
    AAGAAGTTGA AGAAACCTGA TGATATACCA AGCAGGTTTG AGATGAAGCT GCACAGAAAT
    AATATTTTTG AGGAGTCCTA CAGAAGAATC ATGTCTGTGA AAAGACCTGA TGTACTGAAG
    GCCAGGCTCT GGATTGAATT TGAGTCAGAA AAAGGACTTG ACTATGGAGG TGTGGCTAGA
    GAATGGTTTT TTCTCTTGTC CAAGGAGATG TTCAACCCAT ATTATGGTCT CTTTGAATAT
    TCAGCAACGG ACAACTATAC ACTTCAGATT AATCCTAATT CAGGTCTTTG TAACGAAGAT
    CATCTTTCAT ATTTCATGTT TATTGGACGA GTTGCTGGCC TGGCAGTGTA TCATGGGAAA
    CTATTGGATG GCTTCTTCAT CAGACCTTTT TACAAGATGA TGCTGGGGAA GCCAATAATT
    CTGAAAGATA TGGAATCAGT GGACAGTGAA TACTACAACT CTTTGAAGTG GATCCTGGAA
    AATGACCCTA CAGAGCTGGA TCTCATGTTT TGCATTGATG AGGAAAACTT CGGACAGGTG
    TGTAAAGGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.