wnk3 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following wnk3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wnk3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa20448 NM_001005052.1
Latest version!
Xenopus tropicalis WNK lysine deficient protein kinase 3 (wnk3), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $104.30
$149.00
OXa47287 NM_001375262.1
Latest version!
Xenopus tropicalis WNK lysine deficient protein kinase 3 (wnk3), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa63622 XM_031890369.1
Latest version!
Xenopus tropicalis WNK lysine deficient protein kinase 3 (wnk3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa63622 XM_031890371.1
Latest version!
Xenopus tropicalis WNK lysine deficient protein kinase 3 (wnk3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa63622 XM_031890372.1
Latest version!
Xenopus tropicalis WNK lysine deficient protein kinase 3 (wnk3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa63622 XM_031890370.1
Latest version!
Xenopus tropicalis WNK lysine deficient protein kinase 3 (wnk3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa63623 XM_031890373.1
Latest version!
Xenopus tropicalis WNK lysine deficient protein kinase 3 (wnk3), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa20448
    Clone ID Related Accession (Same CDS sequence) NM_001005052.1
    Accession Version NM_001005052.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 567bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-10-27
    Organism Xenopus tropicalis(tropical clawed frog)
    Product serine/threonine-protein kinase WNK3
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC076937.1. ##Evidence-Data-START## Transcript exon combination :: BC076937.1, CF523880.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1696417, SAMN03863235 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGGCTACGG ATTCCGGAGA GCCAGCAAGT ACGGATGAAT CTGACAAAGC AGCTGGCTTT 
    TCATTACAAA ATAATCTGTG CTCCCAGGAA GGTTCCTTAA CCAAACAGGC AAGCATGGAG
    GAAAGTGACA AACCTTGCTC ATCTGCAGGG ACCAGTGAAA AGAAACGTTT CTTTAGAAAA
    AGCGTGGACA TTACAGAGGA TGACAGGATG TTGGAGCAGT CTCTAAAAGA TGAGAAAAGC
    ACTGATCCTC TGAGCCCACA GCTGATGGAG GCACATTCAA CAAAGGCTGC TGGTGCAGCA
    CAAGAAGGGA AAAATGATGC CAAAGCTAAT TCAGAGGCCT GTAAAGACTC TCCTAGAGAG
    AAAACTGAAA GGGAGATGGA GGAAGAGGCT GAAATGAAAG CCGTGGCTAC CTCTCCAAGT
    GGCAGATTTC TAAAGTTTGA CATTGAATTG GGCCGAGGGG CCTTTAAGAC AGTCTTCAAA
    GGACTTGACA CTGAAACTTG GGTAGAAGTT GCCTGGTGTG AGCTGCAGGT GGATCAGCCT
    AACTGTTTGG GAACCTCTGC TTTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001005052.1
    CDS117..683
    Translation

    Target ORF information:

    RefSeq Version NM_001005052.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis WNK lysine deficient protein kinase 3 (wnk3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001005052.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGGCTACGG ATTCCGGAGA GCCAGCAAGT ACGGATGAAT CTGACAAAGC AGCTGGCTTT 
    TCATTACAAA ATAATCTGTG CTCCCAGGAA GGTTCCTTAA CCAAACAGGC AAGCATGGAG
    GAAAGTGACA AACCTTGCTC ATCTGCAGGG ACCAGTGAAA AGAAACGTTT CTTTAGAAAA
    AGCGTGGACA TTACAGAGGA TGACAGGATG TTGGAGCAGT CTCTAAAAGA TGAGAAAAGC
    ACTGATCCTC TGAGCCCACA GCTGATGGAG GCACATTCAA CAAAGGCTGC TGGTGCAGCA
    CAAGAAGGGA AAAATGATGC CAAAGCTAAT TCAGAGGCCT GTAAAGACTC TCCTAGAGAG
    AAAACTGAAA GGGAGATGGA GGAAGAGGCT GAAATGAAAG CCGTGGCTAC CTCTCCAAGT
    GGCAGATTTC TAAAGTTTGA CATTGAATTG GGCCGAGGGG CCTTTAAGAC AGTCTTCAAA
    GGACTTGACA CTGAAACTTG GGTAGAAGTT GCCTGGTGTG AGCTGCAGGT GGATCAGCCT
    AACTGTTTGG GAACCTCTGC TTTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47287
    Clone ID Related Accession (Same CDS sequence) NM_001375262.1
    Accession Version NM_001375262.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8433bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-18
    Organism Xenopus tropicalis(tropical clawed frog)
    Product serine/threonine-protein kinase WNK3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAMC03030488.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1025684, SAMEA1696414 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGGCTACGG ATTCCGGAGA GCCAGCAAGT ACGGATGAAT CTGACAAAGC AGCTGGCTTT 
    TCATTACAAA ATAATCTGTG CTCCCAGGAA GGTTCCTTAA CCAAACAGGC AAGCATGGAG
    GAAAGTGACA AACCTTGCTC ATCTGCAGGG ACCAGTGAAA AGAAACGTTT CTTTAGAAAA
    AGCGTGGACA TTACAGAGGA TGACAGGATG TTGGAGCAGT CTCTAAAAGA TGAGAAAAGC
    ACTGATCCTC TGAGCCCACA GCTGATGGAG GCACATTCAA CAAAGGCTGC TGGTGCAGCA
    CAAGAAGGGA AAAATGATGC CAAAGCTAAT TCAGAGGCCT GTAAAGACTC TCCTAGAGAG
    AAAACTGAAA GGGAGATGGA GGAAGAGGCT GAAATGAAAG CCGTGGCTAC CTCTCCAAGT
    GGCAGATTTC TAAAGTTTGA CATTGAATTG GGCCGAGGGG CCTTTAAGAC AGTCTTCAAA
    GGACTTGACA CTGAAACTTG GGTAGAAGTT GCCTGGTGTG AGCTGCAGGA CAGGAAGTTA
    ACCAAAGCAG AGCAGCAACG ATTTAAAGAA GAGGCCGAGA TGTTAAAGGG CCTTCAGCAC
    CCAAATATTG TGCGGTTTTA TGATTCCTGG GAGTCTTCGC TGAAGGGAAA AAAATGTATA
    GTTTTGGTCA CAGAACTTAT GACATCTGGC ACTTTGAAAA CTTATTTGAA AAGATTTAAG
    GTAATGAAGC CAAAGGTGTT ACGGAGCTGG TGCCGGCAGA TCTTGAAGGG ACTTCAGTTC
    CTGCACACCA GGACACCTCC TATAATCCAT CGTGATCTGA AATGCGACAA CATTTTCATC
    ACTGGTCCCA CAGGCTCAGT GAAGATTGGG GACCTTGGTC TGGCCACCCT GATGCGTACA
    TCATTTGCAA AGAGTGTAAT TGGAACACCA GAATTCATGG CCCCAGAAAT GTATGAAGAG
    CATTACGACG AGTCAGTGGA TGTCTATGCA TTTGGCATGT GCATGCTGGA AATGGCTACA
    TCAGAATACC CATACTCAGA ATGCCAGAAT GCAGCTCAGA TCTACCGCAA AGTAACCAGC
    GGAATCAAAC CTGCCAGCTT CAATAAGGTG TCTGATCCTG AGGTAAAGGA AATAATTGAA
    AGCTGCATCC GTCAGAACAA GGCTGAAAGG TTATCGATAA AAGAACTTCT AAATCATGCT
    TTCTTTGCTG AAGACACTGG CTTGCGAGTT GAACTTGCTG AAGAAGATCA TTGTACAGAT
    TCATCTCTGG CCTTGAGACT TTGGGTGGAG GATCCAAAAA AGCTAAAAGG AAAACATAAA
    GATAATGAAG CTATTGAGTT CAGTTTTAAC TTAGACACGG ACAACCCTGA TGAAGTGGCA
    TGTGAGATGG TGAAATCTGG ATTTTTTCAT GAAAGTGACA GTAAGGCTGT GTCCAAATCT
    ATACGAGACA GAGTGTGTCT TATCAAGAAA ACAAGAGAGC GGAGGATACT GACGGGGTAT
    TCTGAGGACC GGCGTGATTC GCAAAGCAGA ATTGGAGGGG CAACACCTGC TCAGGTAGCA
    GTTCCCATTC CTGCAGTGTA CCAACAACCA GCTGGACACG AGAGTGAAGA AACGGAGGTA
    GACCAGCATG TACGCCAGCA ACTACTGCAA CAGCAGCAGC TTCAGCAGTG CTCTACAGTC
    ACAGTGTGCT CAACAGTCCC TGCTGACAGT CATTCGGAAG CAGGGGCAGG ATCTGTCATG
    CATTCGGATA CTACAAGCCA GCACAGTGCA GTATATGCTA TGAGCCATGA ACAGCTGAGT
    TGCCAGCAAG TGTCTGGGGT GCCTCAGGTA GAAAGTAGTC ATGTGGGGCA AGTCTACCAG
    TCCCAGCAAG TAGTGGGGCA GTATCAACAA ACACAGCAGG TTACTCAGCC GCAAATGCTT
    CCTGCACAAT GCCTTGCTCA GCCTGTCTCA TCTTATCTTC AGTCAGCAGC GGAAGGGCAG
    CTTGGCACAC AGTGCCTGGA TGCGAAGATG ACGTTTGCTG CAAATGGGAA TACTCTTTCG
    CAGTGTAACG CTTTAGATCC TTCAGTGATG TCAGTATTGC AGTCATCCTT TTCTTCGTTG
    TCCCCTAAAA TAATACCTGG ACAAATGACA TCACAGCAAG CAGGGGCTGT TATGCAGCTG
    GATGCTACTA TGACTGGAAT ACAAATTGTT ACACCTGCTG CAACGGACAC CCAAGTTCCT
    GCCCTTCTTC CTGTCTGCCA CCCAGCTTTA CTGGGTCAAT ACAGCAGCAA CATTCAGAAT
    GTGCTGCATC CATCTCAGAC TGTCCATGGG GCACCAGTTG GTCCTCAGCA GACGCAGCAC
    CCTGGAGCAT CTGTGCAGCA ACCAATAATT CCTACTTCAG ATACTTTAAA TTATTCAGCA
    ACGCTTCAGC CCAATATCCA GATACCATCT CACATAGAGC ATCTGCAAAT CTCTCAGCAG
    TCTAGTTACC ATCCTTCACT GCCTCCACAG CAACTTATAT TGCAGCCTGC ACTCACAGAA
    CAACCCCGAA ATCAAAACTT TTCTGATGCT GTTAAAGTTG AAGCTGTCCC ACAACAACTA
    CACATGGAAC CCCATTCAGA ACAGTTAGTA TTCAATGCAA AGACCTCGTC TCTGCATGGT
    GAGATGACAT CATTTGAACC ATCTGGGCAT TTACAGCAGT CTCTCACCTT GAATTCCTTG
    AATGTGCGTG AACAGTCTTC ATATGCCCAG CCTCCATTAC CTCACCTCGG AGTGCAACAA
    CTTTCCAGAG ATGTGGAACA AGTAGCAAAA ACAGTCATGA ACAATCCTCC AGAACAACCA
    CTATACATTC AGATTCCCCC ACTGGACACA TATGGACAAC AGTCTGTTAT TGGTTCTGAT
    CAGATGATAT ATACTCAGAA AGTTATTCCA TCTGATCAGC CTTTATTTTT AGAAAGTACT
    TCCCACTTGA CTAATCAGTC AGTGTATACC CAACAGAGTA TGCAAGCTTC AAATCAACAT
    AATTTTGTGC AACAAGCCAT ACAAACATCT GAGCAGTTGA TATATGCCCA CCCTGCTCTA
    GCGACTCCTG ATCAGACTGT GTACACTGAA AAAAATGCAC CATCAGCAGA ACAGCCAATA
    TTTGTACAGC AGGTTGTCTC GTCTGAACAA CCAGCATACA CACAACAGGT TATATCTGCT
    GAGCAGAAAG GCACACAGCC AGCTATTCCT GCAGAGCAGC CATTGTACAC CCAACAAACT
    GTTCCAGCTG AGCAGCCAGT GTATACTCAG CAAGTTATTC AATCGGAACA GCCAGCTCAC
    GAACAGCAAG GTATACTTGC TCAGCAGCCA GTGTATGCAC AGCAAGGTAT TCCAACTCAA
    CCAGTGTACA TACAGCAAAA TTTTGCAGCT CAGCACCAAG TGTACACACA ACAAAGCAAT
    TCCGCTGAGA CACCAATATA CACACAAAAG AATATTCAAA CTGACCAAGC AGTTTACACA
    CAGCAAGCTG TCGTTAAAAC TGAACAAACA GGATTCACCC AGCATGCTGA GCAAAAAGTG
    CTTGTACTCC AAGCAGACCA GACGATATAC GCACCGCAAC AGCCAATCCA AGTACAGCAA
    GCCGTTCATC TAAAAGAGCA GCCATATTAT GCACAGCAAG CAGCAGCTCA CATTCAGCAG
    TCTGCATCTG CTGAGCAAAC ACAATATCCA CAGTCTGCTT TATCTGCCGA GCAGTCCGTA
    TACTCACAGC AAACTGTATC TGCTGGGCAG TCTGGATATC CACAGCAAGC TTTGTCTGCC
    AAACAACCGC TATATATCCA GCAAGCAGTG TCTGCTGGGC AGCCAGTATA TCCACATCTG
    TCTGTATCCA CTGAGCAGCC ACAATATACA CAACACATTG TTCCCTCTGA TAAACAAATA
    TATACACAGC AAACTGTACC TGCTGAGCAG AGAGTGTACC CTCAGCATAT CGTATCAACT
    GAACAACCAA TGTATTCACA ACAAGTTCTA TCAGCTGAGC GTCCAGCATA TACACATCAA
    GTTGCAGCAC ATCCTGAACG CCTGGTATGC ATCCAACAAG TCCTTCAGCC CACAGAACAC
    CCTGCATATG CTTTACAGTC TATGCCATCC TCAGACAGAC CTGTATATCA ACAAGAATCT
    GCTTTGCTTT CTGGCCATAT GAACACCCAG CCAACTATTC CAGCTCATAC TCAAATTCTG
    TACACACAAG AGGCATTTAT GCCAACAGAA CAGCAGCCAT ATAACCTACA AACTGACAGC
    CCAATATATA TCCAACCAGT TGCTGGTGCT TCTCATCAGG TACCTGAACA ATCGGCCCCT
    ATTGAATCAG TATATAAACA GTCAGCTTCA CAGATGTCAT ATCCTCAGCA TGCAGTATCT
    TCATCTGATA CACAAATGTA TGCAAGCAAG ACGGTGGCTG CAAGTGCTCA GCTTGTGTAT
    GCTGTTCCAC CAGCAGAACA GGTGTACATT CAGCAGACAG GTTCTCTTTC TGAGCAACAA
    CAGTATGCCA GTCAAGCTGC TGTTCAGTCT GACAAGTCAG TTTATAGCTC TCAAACTATA
    CCCCCAGCAC AACAAGCTAT TTATACACAA GCACATCATG CTGTAATATC TGCAGAAAAG
    CCAGTGTTGC ACTCCCAAAA TGTGGAATCT GTTCCTCAGC AGACCTCTTT AAATGCTTCA
    GATGGTCTGT GGTGTATTCA GAAGTCACTG CCTCCTTCAG AGCAGATAGC TTACACTCTG
    CATCCTGCAC CATCTGGAGT GCCATCAGTT TGTGCTCAGC AGATGGTTCC CCTTGAACAG
    CTGGTAAACC CAAATTTGCC ATCTAATATT ATGCCACCTC AAGAGTTTAG GTCAGAGCAG
    CTTACTGAGA GTCAGTTGTA TGCACAACAA CACATTCAGT CTCCAAGACC ACTTCCATCA
    ATAATGCAGT CACAAGAGCA TGGGGCAAGT CACACACCCC CTCCTTTACA GACTGCAGAC
    AATCAAAGCT ATGCAAATTC CTTTGGTAAT CAACAAGGAG GTCAAGTGTT GCTACAGCCA
    GATCTTTTGA ACAAACCTTT ACCTGTCCAA CACCAAGCCT TAACTCAGTT ACCATTAGGT
    CAGTCTGATG TGCATTCATC AGATCAAATG AGCACTGCAT TGCCTTCACA AGAGCCTTTG
    AACCAGGCCT CACATGTGCA GCCACAGCAT ATACCAGGAA CTGCACCTCA AGCCCAAAGT
    AATTCAGCAT TTCAACAGAT GCAACCTTTC TCAAGTCAAA CCCAGTCAAC ATGCATTGAG
    CAACCAACAT CTCTTACGCA TTTGCAGCAA CATCCCCCTG TTCAGGTAGC TGGGACAGAT
    AATCCCCAGT CATGTTCAGG ATCTATGCTT CAACAGATAA TTCCACCTGG GTATGCTTCG
    CAGCCAGTTT CACAACAGGT GTGGCCATCT TCAACTCCAG TCTTTTCAGA ATCTCTCTTA
    AAGCATCAGG ATGTTTACCA AAGTGCGGTG GCGGCACCAC AGACTCAATA CAAAGAACCA
    GAGTCAGAGC AGAGTTTGCA GTCTGCATGC CAAGCAGGGG CACCTGAACA GCATGTGTTT
    CAGAAACAAG ATTCTCTTCA GAGTAATGCT CATGGTGACT GTGCCAAAGA CAGCCCAGGT
    GCACACGACA ACACAGCTGG AAATGGCAAG CAAGATAAAA TGAAACAACG GCGAGCTTCC
    TGCCCCCGGC CTGAAAAAAT AGCCAGATTT ATCCTTACTG TAATGCAGGT ATCAACCTCT
    GGAGATAATA TGGTTGAGTG CCAGCTGGAA ACTCATAACA ACAAGATGGT GACTTTTAAA
    TTTGATGCAG ATGGTGATGC TCCGGAAGAT ATTGCCCATT ATATGGTGGA AGATGACTTT
    GTGCTGGAAG TTGAGAAAGA GAAGTTTGTC GAGGAGCTTA GGAATATAGT TACACAGGCC
    CAGGAGATAC TGCGCAGCAT ACCCGCTGAG GAGAGAACTG AATTGATACA GTCAGAGTCT
    AACAGTCAGG CTGGATCCTC AGATCATGTA CAGATGGGAG TCCCAACATT CCAGCAAACG
    GGGGGTGAAA CGGTTCCACA GTCCTCCCCA GTTGGGCGCT GGAGGTTTTT CATCAATCAG
    ACCATAAGGA ACCGCGAATC TCAGACCTCC TCTGTTTCAC AGTCTGCTGT GATCAGGGTT
    CCTCAATTAG CCACATTAGA TAATGAACAA GCAAGTCTCC CTAAGGATTC TTCAGCAGAC
    AGCTGTTCTC TTAAATCTGA GCATACAGTG CCGACTGTTC TTCTTCAGCC AACAGATGGT
    CATGCTTTCC CTACTGAGAC TGAACCTTTG ACTCTCCCTA CTTATGTGAC AGTTGGAAGT
    GAAATGTTAT CATCTCCATT GGACTGTAAT GCCTCAACTT TAGGTCAGAT TCAGCTAGAA
    CCTAGAAACT GTGGTAGTAA TATACTTGTG CTAGCAGAGA GCCAGCAGCC AGAAATACCT
    TCATCTTTTG ATCAGGACGT CCAAGGAGCA AATGGCCTGG TTAACACAAC TGAGACCAGT
    ACTGGACACT TACCGGCACA AAGCACTACA GGCTTGTTAC TTGGCTTAAC TATGTCAGAT
    GCTCTTCATC CAGTTGATAC AACTGTGTTG CCCCAGACTC CCTTGTCAGA AGTTTCCTTA
    GCGCACTCTG CATCAGTGCA AGAGTCTGAC ACTGAAGGAC CCCCTAAAAT AGACTATGTG
    GATAATCACA TCAAAACGTT GGATGAGAAA TTAAGAACTT TGTTATATCA GGACAGCTCT
    GCCAGCTCTT ATGCTGACAG CCAAAAGGAA ACACAGAGTA TAGAATCTCC CTTATCTTCA
    TCTGCTGAGG ATACTTTGTC GTGTCCTGTC CATGAAGCCC TCGATGTGAA TACTGCCGGT
    GTGCAGGCCA CTCCAGAGGA GGCAGACGCT AATGACCCTC CAGCGCAAAG AGATCCTGGA
    GAAGTCATCA CAGTACCTGG TACACTAACA CCATCAGAGA GTTCAGAAGA TGCCTGGGTT
    CCTGGAAGTT CCTCCCACGC TTGTTCCAAG CGCTCTATGG GTACTGGAGC CACGCATCTG
    CAATCAGGAG TTGAAGAGGA GGGAGTGAGA CTGGACATAT CTGATGCCAC ACAAAGAGTT
    GTTGAAGCAT TTGCTGAATG TGCCTTGTCA GAAGGGTCGC CATCAAAAAA CGGCACCTTC
    AAGCGTGGCC GTTTCCAGGT TGTTTCCGTT CCACAGCAAG AGCAGCCCAC TGCAAATGAA
    TCAGCTGGTG CTCCAGATAC CAAATACCCA GGTAACTCTC TTTTACAAGA AACTGGATCC
    GCAAATACAA AAGCAGAAGA CACTCCGCAG TCTGCCAGCA CAGCATGTGA GACTGATGCC
    TCCTCCCTCA CACCTGATAG AGAACTGGAT GAAACCTCAG CCAATGGCAG CAGTGTTCAG
    TCAAGTTCAG CCTTGTGGAT GAAAGATATC AAGAAGCCGA GCAGCTGTAA TAAACAGCTA
    AGCAGTGATT CTGAACAATC TACTACTCCG GACAGGCCCC TGGAATTTAA AGGGAGAGAA
    AAAGATGCGG GGCAGCAACC CCAGGGAGAG AAAATGTATA ACCAGAAACA GAATTCCTTC
    TTTCACTCTC CATCTTCCCC CATGAGCAGT GATGACGAGT CCGAGTTAGA AGATGAAGAT
    TTGAAAGTGG AACTTCAGAA GCTCCGAGAA AAGCACATTC AAGAGGTGGT GACCCTTCAA
    ACCCAGCAAA ACCACGAGCT CCAGGAGCTG TATGTACGCT TGAAGTCTTT AAGGGAAAGC
    AAAGCACAGT CTTCAGATAC CTCTTCCCAG CCATTATCCC CCCGTCGCCC AAGGTCTCTA
    AAGAGTAAAC TGCGCAGCAG GCCCCAGTCC CTCACTCACA TGGACAATGG GATCGGGCTT
    TCAGACCACC AGTGCAGCGA GAGCAATTCC GATGCTTGCC AGCAGTCCTT GTCTGAGAAG
    AAGAGCATGT TCACGGATGA CTTTCATAAG CTGGTGGATG ACTGGGCAAA GGAAAATGCT
    GGCAACCAAG TTTTGAAACC CAGTCTGAAT CAGATCAAGC AGAATCAAAG TCGCCTAGAG
    TCTGATAACT GGGGTCGGAC CTATGAGAGT ATGACAGCCA CATCAGGCTA TCCACCTGCA
    TGGATCCCTC AGATTCATGG CTCTTTGCCA GCTGCTGTCT CTCAGTCTCT CGTGCCCACC
    AACTTTACAG CTGGAGCGAT GCCAGCATAC CAAGTGCCAC CAGCCTGCCA ATTCACTTCA
    GTGAGCAGTA GTGGTTATCC TGTACCATGG TCTGCGCAAG CACCAGTAGT CCCTCCCCAG
    CATCTCACAG CATACCAGCC TGGCGTCGGA GTTCAAACTT TTGCAACCTC AACAGCTCAG
    AAGGCAACTA CAATCCCAAC ATCTCCAAAA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001362191.1
    CDS143..8575
    Translation

    Target ORF information:

    RefSeq Version NM_001375262.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis WNK lysine deficient protein kinase 3 (wnk3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001375262.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGGCTACGG ATTCCGGAGA GCCAGCAAGT ACGGATGAAT CTGACAAAGC AGCTGGCTTT 
    TCATTACAAA ATAATCTGTG CTCCCAGGAA GGTTCCTTAA CCAAACAGGC AAGCATGGAG
    GAAAGTGACA AACCTTGCTC ATCTGCAGGG ACCAGTGAAA AGAAACGTTT CTTTAGAAAA
    AGCGTGGACA TTACAGAGGA TGACAGGATG TTGGAGCAGT CTCTAAAAGA TGAGAAAAGC
    ACTGATCCTC TGAGCCCACA GCTGATGGAG GCACATTCAA CAAAGGCTGC TGGTGCAGCA
    CAAGAAGGGA AAAATGATGC CAAAGCTAAT TCAGAGGCCT GTAAAGACTC TCCTAGAGAG
    AAAACTGAAA GGGAGATGGA GGAAGAGGCT GAAATGAAAG CCGTGGCTAC CTCTCCAAGT
    GGCAGATTTC TAAAGTTTGA CATTGAATTG GGCCGAGGGG CCTTTAAGAC AGTCTTCAAA
    GGACTTGACA CTGAAACTTG GGTAGAAGTT GCCTGGTGTG AGCTGCAGGA CAGGAAGTTA
    ACCAAAGCAG AGCAGCAACG ATTTAAAGAA GAGGCCGAGA TGTTAAAGGG CCTTCAGCAC
    CCAAATATTG TGCGGTTTTA TGATTCCTGG GAGTCTTCGC TGAAGGGAAA AAAATGTATA
    GTTTTGGTCA CAGAACTTAT GACATCTGGC ACTTTGAAAA CTTATTTGAA AAGATTTAAG
    GTAATGAAGC CAAAGGTGTT ACGGAGCTGG TGCCGGCAGA TCTTGAAGGG ACTTCAGTTC
    CTGCACACCA GGACACCTCC TATAATCCAT CGTGATCTGA AATGCGACAA CATTTTCATC
    ACTGGTCCCA CAGGCTCAGT GAAGATTGGG GACCTTGGTC TGGCCACCCT GATGCGTACA
    TCATTTGCAA AGAGTGTAAT TGGAACACCA GAATTCATGG CCCCAGAAAT GTATGAAGAG
    CATTACGACG AGTCAGTGGA TGTCTATGCA TTTGGCATGT GCATGCTGGA AATGGCTACA
    TCAGAATACC CATACTCAGA ATGCCAGAAT GCAGCTCAGA TCTACCGCAA AGTAACCAGC
    GGAATCAAAC CTGCCAGCTT CAATAAGGTG TCTGATCCTG AGGTAAAGGA AATAATTGAA
    AGCTGCATCC GTCAGAACAA GGCTGAAAGG TTATCGATAA AAGAACTTCT AAATCATGCT
    TTCTTTGCTG AAGACACTGG CTTGCGAGTT GAACTTGCTG AAGAAGATCA TTGTACAGAT
    TCATCTCTGG CCTTGAGACT TTGGGTGGAG GATCCAAAAA AGCTAAAAGG AAAACATAAA
    GATAATGAAG CTATTGAGTT CAGTTTTAAC TTAGACACGG ACAACCCTGA TGAAGTGGCA
    TGTGAGATGG TGAAATCTGG ATTTTTTCAT GAAAGTGACA GTAAGGCTGT GTCCAAATCT
    ATACGAGACA GAGTGTGTCT TATCAAGAAA ACAAGAGAGC GGAGGATACT GACGGGGTAT
    TCTGAGGACC GGCGTGATTC GCAAAGCAGA ATTGGAGGGG CAACACCTGC TCAGGTAGCA
    GTTCCCATTC CTGCAGTGTA CCAACAACCA GCTGGACACG AGAGTGAAGA AACGGAGGTA
    GACCAGCATG TACGCCAGCA ACTACTGCAA CAGCAGCAGC TTCAGCAGTG CTCTACAGTC
    ACAGTGTGCT CAACAGTCCC TGCTGACAGT CATTCGGAAG CAGGGGCAGG ATCTGTCATG
    CATTCGGATA CTACAAGCCA GCACAGTGCA GTATATGCTA TGAGCCATGA ACAGCTGAGT
    TGCCAGCAAG TGTCTGGGGT GCCTCAGGTA GAAAGTAGTC ATGTGGGGCA AGTCTACCAG
    TCCCAGCAAG TAGTGGGGCA GTATCAACAA ACACAGCAGG TTACTCAGCC GCAAATGCTT
    CCTGCACAAT GCCTTGCTCA GCCTGTCTCA TCTTATCTTC AGTCAGCAGC GGAAGGGCAG
    CTTGGCACAC AGTGCCTGGA TGCGAAGATG ACGTTTGCTG CAAATGGGAA TACTCTTTCG
    CAGTGTAACG CTTTAGATCC TTCAGTGATG TCAGTATTGC AGTCATCCTT TTCTTCGTTG
    TCCCCTAAAA TAATACCTGG ACAAATGACA TCACAGCAAG CAGGGGCTGT TATGCAGCTG
    GATGCTACTA TGACTGGAAT ACAAATTGTT ACACCTGCTG CAACGGACAC CCAAGTTCCT
    GCCCTTCTTC CTGTCTGCCA CCCAGCTTTA CTGGGTCAAT ACAGCAGCAA CATTCAGAAT
    GTGCTGCATC CATCTCAGAC TGTCCATGGG GCACCAGTTG GTCCTCAGCA GACGCAGCAC
    CCTGGAGCAT CTGTGCAGCA ACCAATAATT CCTACTTCAG ATACTTTAAA TTATTCAGCA
    ACGCTTCAGC CCAATATCCA GATACCATCT CACATAGAGC ATCTGCAAAT CTCTCAGCAG
    TCTAGTTACC ATCCTTCACT GCCTCCACAG CAACTTATAT TGCAGCCTGC ACTCACAGAA
    CAACCCCGAA ATCAAAACTT TTCTGATGCT GTTAAAGTTG AAGCTGTCCC ACAACAACTA
    CACATGGAAC CCCATTCAGA ACAGTTAGTA TTCAATGCAA AGACCTCGTC TCTGCATGGT
    GAGATGACAT CATTTGAACC ATCTGGGCAT TTACAGCAGT CTCTCACCTT GAATTCCTTG
    AATGTGCGTG AACAGTCTTC ATATGCCCAG CCTCCATTAC CTCACCTCGG AGTGCAACAA
    CTTTCCAGAG ATGTGGAACA AGTAGCAAAA ACAGTCATGA ACAATCCTCC AGAACAACCA
    CTATACATTC AGATTCCCCC ACTGGACACA TATGGACAAC AGTCTGTTAT TGGTTCTGAT
    CAGATGATAT ATACTCAGAA AGTTATTCCA TCTGATCAGC CTTTATTTTT AGAAAGTACT
    TCCCACTTGA CTAATCAGTC AGTGTATACC CAACAGAGTA TGCAAGCTTC AAATCAACAT
    AATTTTGTGC AACAAGCCAT ACAAACATCT GAGCAGTTGA TATATGCCCA CCCTGCTCTA
    GCGACTCCTG ATCAGACTGT GTACACTGAA AAAAATGCAC CATCAGCAGA ACAGCCAATA
    TTTGTACAGC AGGTTGTCTC GTCTGAACAA CCAGCATACA CACAACAGGT TATATCTGCT
    GAGCAGAAAG GCACACAGCC AGCTATTCCT GCAGAGCAGC CATTGTACAC CCAACAAACT
    GTTCCAGCTG AGCAGCCAGT GTATACTCAG CAAGTTATTC AATCGGAACA GCCAGCTCAC
    GAACAGCAAG GTATACTTGC TCAGCAGCCA GTGTATGCAC AGCAAGGTAT TCCAACTCAA
    CCAGTGTACA TACAGCAAAA TTTTGCAGCT CAGCACCAAG TGTACACACA ACAAAGCAAT
    TCCGCTGAGA CACCAATATA CACACAAAAG AATATTCAAA CTGACCAAGC AGTTTACACA
    CAGCAAGCTG TCGTTAAAAC TGAACAAACA GGATTCACCC AGCATGCTGA GCAAAAAGTG
    CTTGTACTCC AAGCAGACCA GACGATATAC GCACCGCAAC AGCCAATCCA AGTACAGCAA
    GCCGTTCATC TAAAAGAGCA GCCATATTAT GCACAGCAAG CAGCAGCTCA CATTCAGCAG
    TCTGCATCTG CTGAGCAAAC ACAATATCCA CAGTCTGCTT TATCTGCCGA GCAGTCCGTA
    TACTCACAGC AAACTGTATC TGCTGGGCAG TCTGGATATC CACAGCAAGC TTTGTCTGCC
    AAACAACCGC TATATATCCA GCAAGCAGTG TCTGCTGGGC AGCCAGTATA TCCACATCTG
    TCTGTATCCA CTGAGCAGCC ACAATATACA CAACACATTG TTCCCTCTGA TAAACAAATA
    TATACACAGC AAACTGTACC TGCTGAGCAG AGAGTGTACC CTCAGCATAT CGTATCAACT
    GAACAACCAA TGTATTCACA ACAAGTTCTA TCAGCTGAGC GTCCAGCATA TACACATCAA
    GTTGCAGCAC ATCCTGAACG CCTGGTATGC ATCCAACAAG TCCTTCAGCC CACAGAACAC
    CCTGCATATG CTTTACAGTC TATGCCATCC TCAGACAGAC CTGTATATCA ACAAGAATCT
    GCTTTGCTTT CTGGCCATAT GAACACCCAG CCAACTATTC CAGCTCATAC TCAAATTCTG
    TACACACAAG AGGCATTTAT GCCAACAGAA CAGCAGCCAT ATAACCTACA AACTGACAGC
    CCAATATATA TCCAACCAGT TGCTGGTGCT TCTCATCAGG TACCTGAACA ATCGGCCCCT
    ATTGAATCAG TATATAAACA GTCAGCTTCA CAGATGTCAT ATCCTCAGCA TGCAGTATCT
    TCATCTGATA CACAAATGTA TGCAAGCAAG ACGGTGGCTG CAAGTGCTCA GCTTGTGTAT
    GCTGTTCCAC CAGCAGAACA GGTGTACATT CAGCAGACAG GTTCTCTTTC TGAGCAACAA
    CAGTATGCCA GTCAAGCTGC TGTTCAGTCT GACAAGTCAG TTTATAGCTC TCAAACTATA
    CCCCCAGCAC AACAAGCTAT TTATACACAA GCACATCATG CTGTAATATC TGCAGAAAAG
    CCAGTGTTGC ACTCCCAAAA TGTGGAATCT GTTCCTCAGC AGACCTCTTT AAATGCTTCA
    GATGGTCTGT GGTGTATTCA GAAGTCACTG CCTCCTTCAG AGCAGATAGC TTACACTCTG
    CATCCTGCAC CATCTGGAGT GCCATCAGTT TGTGCTCAGC AGATGGTTCC CCTTGAACAG
    CTGGTAAACC CAAATTTGCC ATCTAATATT ATGCCACCTC AAGAGTTTAG GTCAGAGCAG
    CTTACTGAGA GTCAGTTGTA TGCACAACAA CACATTCAGT CTCCAAGACC ACTTCCATCA
    ATAATGCAGT CACAAGAGCA TGGGGCAAGT CACACACCCC CTCCTTTACA GACTGCAGAC
    AATCAAAGCT ATGCAAATTC CTTTGGTAAT CAACAAGGAG GTCAAGTGTT GCTACAGCCA
    GATCTTTTGA ACAAACCTTT ACCTGTCCAA CACCAAGCCT TAACTCAGTT ACCATTAGGT
    CAGTCTGATG TGCATTCATC AGATCAAATG AGCACTGCAT TGCCTTCACA AGAGCCTTTG
    AACCAGGCCT CACATGTGCA GCCACAGCAT ATACCAGGAA CTGCACCTCA AGCCCAAAGT
    AATTCAGCAT TTCAACAGAT GCAACCTTTC TCAAGTCAAA CCCAGTCAAC ATGCATTGAG
    CAACCAACAT CTCTTACGCA TTTGCAGCAA CATCCCCCTG TTCAGGTAGC TGGGACAGAT
    AATCCCCAGT CATGTTCAGG ATCTATGCTT CAACAGATAA TTCCACCTGG GTATGCTTCG
    CAGCCAGTTT CACAACAGGT GTGGCCATCT TCAACTCCAG TCTTTTCAGA ATCTCTCTTA
    AAGCATCAGG ATGTTTACCA AAGTGCGGTG GCGGCACCAC AGACTCAATA CAAAGAACCA
    GAGTCAGAGC AGAGTTTGCA GTCTGCATGC CAAGCAGGGG CACCTGAACA GCATGTGTTT
    CAGAAACAAG ATTCTCTTCA GAGTAATGCT CATGGTGACT GTGCCAAAGA CAGCCCAGGT
    GCACACGACA ACACAGCTGG AAATGGCAAG CAAGATAAAA TGAAACAACG GCGAGCTTCC
    TGCCCCCGGC CTGAAAAAAT AGCCAGATTT ATCCTTACTG TAATGCAGGT ATCAACCTCT
    GGAGATAATA TGGTTGAGTG CCAGCTGGAA ACTCATAACA ACAAGATGGT GACTTTTAAA
    TTTGATGCAG ATGGTGATGC TCCGGAAGAT ATTGCCCATT ATATGGTGGA AGATGACTTT
    GTGCTGGAAG TTGAGAAAGA GAAGTTTGTC GAGGAGCTTA GGAATATAGT TACACAGGCC
    CAGGAGATAC TGCGCAGCAT ACCCGCTGAG GAGAGAACTG AATTGATACA GTCAGAGTCT
    AACAGTCAGG CTGGATCCTC AGATCATGTA CAGATGGGAG TCCCAACATT CCAGCAAACG
    GGGGGTGAAA CGGTTCCACA GTCCTCCCCA GTTGGGCGCT GGAGGTTTTT CATCAATCAG
    ACCATAAGGA ACCGCGAATC TCAGACCTCC TCTGTTTCAC AGTCTGCTGT GATCAGGGTT
    CCTCAATTAG CCACATTAGA TAATGAACAA GCAAGTCTCC CTAAGGATTC TTCAGCAGAC
    AGCTGTTCTC TTAAATCTGA GCATACAGTG CCGACTGTTC TTCTTCAGCC AACAGATGGT
    CATGCTTTCC CTACTGAGAC TGAACCTTTG ACTCTCCCTA CTTATGTGAC AGTTGGAAGT
    GAAATGTTAT CATCTCCATT GGACTGTAAT GCCTCAACTT TAGGTCAGAT TCAGCTAGAA
    CCTAGAAACT GTGGTAGTAA TATACTTGTG CTAGCAGAGA GCCAGCAGCC AGAAATACCT
    TCATCTTTTG ATCAGGACGT CCAAGGAGCA AATGGCCTGG TTAACACAAC TGAGACCAGT
    ACTGGACACT TACCGGCACA AAGCACTACA GGCTTGTTAC TTGGCTTAAC TATGTCAGAT
    GCTCTTCATC CAGTTGATAC AACTGTGTTG CCCCAGACTC CCTTGTCAGA AGTTTCCTTA
    GCGCACTCTG CATCAGTGCA AGAGTCTGAC ACTGAAGGAC CCCCTAAAAT AGACTATGTG
    GATAATCACA TCAAAACGTT GGATGAGAAA TTAAGAACTT TGTTATATCA GGACAGCTCT
    GCCAGCTCTT ATGCTGACAG CCAAAAGGAA ACACAGAGTA TAGAATCTCC CTTATCTTCA
    TCTGCTGAGG ATACTTTGTC GTGTCCTGTC CATGAAGCCC TCGATGTGAA TACTGCCGGT
    GTGCAGGCCA CTCCAGAGGA GGCAGACGCT AATGACCCTC CAGCGCAAAG AGATCCTGGA
    GAAGTCATCA CAGTACCTGG TACACTAACA CCATCAGAGA GTTCAGAAGA TGCCTGGGTT
    CCTGGAAGTT CCTCCCACGC TTGTTCCAAG CGCTCTATGG GTACTGGAGC CACGCATCTG
    CAATCAGGAG TTGAAGAGGA GGGAGTGAGA CTGGACATAT CTGATGCCAC ACAAAGAGTT
    GTTGAAGCAT TTGCTGAATG TGCCTTGTCA GAAGGGTCGC CATCAAAAAA CGGCACCTTC
    AAGCGTGGCC GTTTCCAGGT TGTTTCCGTT CCACAGCAAG AGCAGCCCAC TGCAAATGAA
    TCAGCTGGTG CTCCAGATAC CAAATACCCA GGTAACTCTC TTTTACAAGA AACTGGATCC
    GCAAATACAA AAGCAGAAGA CACTCCGCAG TCTGCCAGCA CAGCATGTGA GACTGATGCC
    TCCTCCCTCA CACCTGATAG AGAACTGGAT GAAACCTCAG CCAATGGCAG CAGTGTTCAG
    TCAAGTTCAG CCTTGTGGAT GAAAGATATC AAGAAGCCGA GCAGCTGTAA TAAACAGCTA
    AGCAGTGATT CTGAACAATC TACTACTCCG GACAGGCCCC TGGAATTTAA AGGGAGAGAA
    AAAGATGCGG GGCAGCAACC CCAGGGAGAG AAAATGTATA ACCAGAAACA GAATTCCTTC
    TTTCACTCTC CATCTTCCCC CATGAGCAGT GATGACGAGT CCGAGTTAGA AGATGAAGAT
    TTGAAAGTGG AACTTCAGAA GCTCCGAGAA AAGCACATTC AAGAGGTGGT GACCCTTCAA
    ACCCAGCAAA ACCACGAGCT CCAGGAGCTG TATGTACGCT TGAAGTCTTT AAGGGAAAGC
    AAAGCACAGT CTTCAGATAC CTCTTCCCAG CCATTATCCC CCCGTCGCCC AAGGTCTCTA
    AAGAGTAAAC TGCGCAGCAG GCCCCAGTCC CTCACTCACA TGGACAATGG GATCGGGCTT
    TCAGACCACC AGTGCAGCGA GAGCAATTCC GATGCTTGCC AGCAGTCCTT GTCTGAGAAG
    AAGAGCATGT TCACGGATGA CTTTCATAAG CTGGTGGATG ACTGGGCAAA GGAAAATGCT
    GGCAACCAAG TTTTGAAACC CAGTCTGAAT CAGATCAAGC AGAATCAAAG TCGCCTAGAG
    TCTGATAACT GGGGTCGGAC CTATGAGAGT ATGACAGCCA CATCAGGCTA TCCACCTGCA
    TGGATCCCTC AGATTCATGG CTCTTTGCCA GCTGCTGTCT CTCAGTCTCT CGTGCCCACC
    AACTTTACAG CTGGAGCGAT GCCAGCATAC CAAGTGCCAC CAGCCTGCCA ATTCACTTCA
    GTGAGCAGTA GTGGTTATCC TGTACCATGG TCTGCGCAAG CACCAGTAGT CCCTCCCCAG
    CATCTCACAG CATACCAGCC TGGCGTCGGA GTTCAAACTT TTGCAACCTC AACAGCTCAG
    AAGGCAACTA CAATCCCAAC ATCTCCAAAA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa63622
    Clone ID Related Accession (Same CDS sequence) XM_031890370.1 , XM_031890369.1 , XM_031890371.1 , XM_031890372.1
    Accession Version XM_031890370.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8574bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product serine/threonine-protein kinase WNK3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCTACGG ATTCCGGAGA GCCAGCAAGT ACGGATGAAT CTGACAAAGC AGCTGGCTTT 
    TCATTACAAA ATAATCTGTG CTCCCAGGAA GGTTCCTTAA CCAAACAGGC AAGCATGGAG
    GAAAGTGACA AACCTTGCTC ATCTGCAGGG ACCAGTGAAA AGAAACGTTT CTTTAGAAAA
    AGCGTGGACA TTACAGAGGA TGACAGGATG TTGGAGCAGT CTCTAAAAGA TGAGAAAAGC
    ACTGATCCTC TGAGCCCACA GCTGATGGAG GCACATTCAA CAAAGGCTGC TGGTGCAGCA
    CAAGAAGGGA AAAATGATGC CAAAGCTAAT TCAGAGGCCT GTAAAGACTC TCCTAGAGAG
    AAAACTGAAA GGGAGATGGA GGAAGAGGCT GAAATGAAAG CCGTGGCTAC CTCTCCAAGT
    GGCAGATTTC TAAAGTTTGA CATTGAATTG GGCCGAGGGG CCTTTAAGAC AGTCTTCAAA
    GGACTTGACA CTGAAACTTG GGTAGAAGTT GCCTGGTGTG AGCTGCAGGA CAGGAAGTTA
    ACCAAAGCAG AGCAGCAACG ATTTAAAGAA GAGGCCGAGA TGTTAAAGGG CCTTCAGCAC
    CCAAATATTG TGCGGTTTTA TGATTCCTGG GAGTCTTCGC TGAAGGGAAA AAAATGTATA
    GTTTTGGTCA CAGAACTTAT GACATCTGGC ACTTTGAAAA CTTATTTGAA AAGATTTAAG
    GTAATGAAGC CAAAGGTGTT ACGGAGCTGG TGCCGGCAGA TCTTGAAGGG ACTTCAGTTC
    CTGCACACCA GGACACCTCC TATAATCCAT CGTGATCTGA AATGCGACAA CATTTTCATC
    ACTGGTCCCA CAGGCTCAGT GAAGATTGGG GACCTTGGTC TGGCCACCCT GATGCGTACA
    TCATTTGCAA AGAGTGTAAT TGGAACACCA GAATTCATGG CCCCAGAAAT GTATGAAGAG
    CATTACGACG AGTCAGTGGA TGTCTATGCA TTTGGCATGT GCATGCTGGA AATGGCTACA
    TCAGAATACC CATACTCAGA ATGCCAGAAT GCAGCTCAGA TCTACCGCAA AGTAACCAGC
    GGAATCAAAC CTGCCAGCTT CAATAAGGTG TCTGATCCTG AGGTAAAGGA AATAATTGAA
    AGCTGCATCC GTCAGAACAA GGCTGAAAGG TTATCGATAA AAGAACTTCT AAATCATGCT
    TTCTTTGCTG AAGACACTGG CTTGCGAGTT GAACTTGCTG AAGAAGATCA TTGTACAGAT
    TCATCTCTGG CCTTGAGACT TTGGGTGGAG GATCCAAAAA AGCTAAAAGG AAAACATAAA
    GATAATGAAG CTATTGAGTT CAGTTTTAAC TTAGACACGG ACAACCCTGA TGAAGTGGCA
    TGTGAGATGG TGAAATCTGG ATTTTTTCAT GAAAGTGACA GTAAGGCTGT GTCCAAATCT
    ATACGAGACA GAGTGTGTCT TATCAAGAAA ACAAGAGAGC GGAGGATACT GACGGGGTAT
    TCTGAGGACC GGCGTGATTC GCAAAGCAGA ATTGGAGGGG CAACACCTGC TCAGGTAGCA
    GTTCCCATTC CTGCAGTGTA CCAACAACCA GCTGGACACG AGAGTGAAGA AACGGAGGTA
    GACCAGCATG TACGCCAGCA ACTACTGCAA CAGCAGCAGC TTCAGCAGTG CTCTACAGTC
    ACAGTGTGCT CAACAGTCCC TGCTGACAGT CATTCGGAAG CAGGGGCAGG ATCTGTCATG
    CATTCGGATA CTACAAGCCA GCACAGTGCA GTATATGCTA TGAGCCATGA ACAGCTGAGT
    TGCCAGCAAG TGTCTGGGGT GCCTCAGGTA GAAAGTAGTC ATGTGGGGCA AGTCTACCAG
    TCCCAGCAAG TAGTGGGGCA GTATCAACAA ACACAGCAGG TTACTCAGCC GCAAATGCTT
    CCTGCACAAT GCCTTGCTCA GCCTGTCTCA TCTTATCTTC AGTCAGCAGC GGAAGGGCAG
    CTTGGCACAC AGTGCCTGGA TGCGAAGATG ACGTTTGCTG CAAATGGGAA TACTCTTTCG
    CAGTGTAACG CTTTAGATCC TTCAGTGATG TCAGTATTGC AGTCATCCTT TTCTTCGTTG
    TCCCCTAAAA TAATACCTGG ACAAATGACA TCACAGCAAG CAGGGGCTGT TATGCAGCTG
    GATGCTACTA TGACTGGAAT ACAAATTGTT ACACCTGCTG CAACGGACAC CCAAGTTCCT
    GCCCTTCTTC CTGTCTGCCA CCCAGCTTTA CTGGGTCAAT ACAGCAGCAA CATTCAGAAT
    GTGCTGCATC CATCTCAGAC TGTCCATGGG GCACCAGTTG GTCCTCAGCA GACGCAGCAC
    CCTGGAGCAT CTGTGCAGCA ACCAATAATT CCTACTTCAG ATACTTTAAA TTATTCAGCA
    ACGCTTCAGC CCAATATCCA GATACCATCT CACATAGAGC ATCTGCAAAT CTCTCAGCAG
    TCTAGTTACC ATCCTTCACT GCCTCCACAG CAACTTATAT TGCAGCCTGC ACTCACAGAA
    CAACCCCGAA ATCAAAACTT TTCTGATGCT GTTAAAGTTG AAGCTGTCCC ACAACAACTA
    CACATGGAAC CCCATTCAGA ACAGTTAGTA TTCAATGCAA AGACCTCGTC TCTGCATGGT
    GAGATGACAT CATTTGAACC ATCTGGGCAT TTACAGCAGT CTCTCACCTT GAATTCCTTG
    AATGTGCGTG AACAGTCTTC ATATGCCCAG CCTCCATTAC CTCACCTCGG AGTGCAACAA
    CTTTCCAGAG ATGTGGAACA AGTAGCAAAA ACAGTCATGA ACAATCCTCC AGAACAACCA
    CTATACATTC AGATTCCCCC ACTGGACACA TATGGACAAC AGTCTGTTAT TGGTTCTGAT
    CAGATGATAT ATACTCAGAA AGTTATTCCA TCTGATCAGC CTTTATTTTT AGAAAGTACT
    TCCCACTTGA CTAATCAGTC AGTGTATACC CAACAGAGTA TGCAAGCTTC AAATCAACAT
    AATTTTGTGC AACAAGCCAT ACAAACATCT GAGCAGTTGA TATATGCCCA CCCTGCTCTA
    GCGACTCCTG ATCAGACTGT GTACACTGAA AAAAATGCAC CATCAGCAGA ACAGCCAATA
    TTTGTACAGC AGGTTGTCTC GTCTGAACAA CCAGCATACA CACAACAGGT TATATCTGCT
    GAGCAGAAAG GCACACAGCC AGCTATTCCT GCAGAGCAGC CATTGTACAC CCAACAAACT
    GTTCCAGCTG AGCAGCCAGT GTATACTCAG CAAGTTATTC AATCGGAACA GCCAGCTCAC
    GAACAGCAAG GTATACTTGC TCAGCAGCCA GTGTATGCAC AGCAAGGTAT TCCAACTCAA
    CCAGTGTACA TACAGCAAAA TTTTGCAGCT CAGCACCAAG TGTACACACA ACAAAGCAAT
    TCCGCTGAGA CACCAATATA CACACAAAAG AATATTCAAA CTGACCAAGC AGTTTACACA
    CAGCAAGCTG TCGTTAAAAC TGAACAAACA GGATTCACCC AGCATGCTGA GCAAAAAGTG
    CTTGTACTCC AAGCAGACCA GACGATATAC GCACCGCAAC AGCCAATCCA AGTACAGCAA
    GCCGTTCATC TAAAAGAGCA GCCATATTAT GCACAGCAAG CAGCAGCTCA CATTCAGCAG
    TCTGCATCTG CTGAGCAAAC ACAATATCCA CAGTCTGCTT TATCTGCCGA GCAGTCCGTA
    TACTCACAGC AAACTGTATC TGCTGGGCAG TCTGGATATC CACAGCAAGC TTTGTCTGCC
    AAACAACCGC TATATATCCA GCAAGCAGTG TCTGCTGGGC AGCCAGTATA TCCACATCTG
    TCTGTATCCA CTGAGCAGCC ACAATATACA CAACACATTG TTCCCTCTGA TAAACAAATA
    TATACACAGC AAACTGTACC TGCTGAGCAG AGAGTGTACC CTCAGCATAT CGTATCAACT
    GAACAACCAA TGTATTCACA ACAAGTTCTA TCAGCTGAGC GTCCAGCATA TACACATCAA
    GTTGCAGCAC ATCCTGAACG CCTGGTATGC ATCCAACAAG TCCTTCAGCC CACAGAACAC
    CCTGCATATG CTTTACAGTC TATGCCATCC TCAGACAGAC CTGTATATCA ACAAGAATCT
    GCTTTGCTTT CTGGCCATAT GAACACCCAG CCAACTATTC CAGCTCATAC TCAAATTCTG
    TACACACAAG AGGCATTTAT GCCAACAGAA CAGCAGCCAT ATAACCTACA AACTGACAGC
    CCAATATATA TCCAACCAGT TGCTGGTGCT TCTCATCAGG TACCTGAACA ATCGGCCCCT
    ATTGAATCAG TATATAAACA GTCAGCTTCA CAGATGTCAT ATCCTCAGCA TGCAGTATCT
    TCATCTGATA CACAAATGTA TGCAAGCAAG ACGGTGGCTG CAAGTGCTCA GCTTGTGTAT
    GCTGTTCCAC CAGCAGAACA GGTGTACATT CAGCAGACAG GTTCTCTTTC TGAGCAACAA
    CAGTATGCCA GTCAAGCTGC TGTTCAGTCT GACAAGTCAG TTTATAGCTC TCAAACTATA
    CCCCCAGCAC AACAAGCTAT TTATACACAA GCACATCATG CTGTAATATC TGCAGAAAAG
    CCAGTGTTGC ACTCCCAAAA TGTGGAATCT GTTCCTCAGC AGACCTCTTT AAATGCTTCA
    GATGGTCTGT GGTGTATTCA GAAGTCACTG CCTCCTTCAG AGCAGATAGC TTACACTCTG
    CATCCTGCAC CATCTGGAGT GCCATCAGTT TGTGCTCAGC AGATGGTTCC CCTTGAACAG
    CTGGTAAACC CAAATTTGCC ATCTAATATT ATGCCACCTC AAGAGTTTAG GTCAGAGCAG
    CTTACTGAGA GTCAGTTGTA TGCACAACAA CACATTCAGT CTCCAAGACC ACTTCCATCA
    ATAATGCAGT CACAAGAGCA TGGGGCAAGT CACACACCCC CTCCTTTACA GACTGCAGAC
    AATCAAAGCT ATGCAAATTC CTTTGGTAAT CAACAAGGAG GTCAAGTGTT GCTACAGCCA
    GATCTTTTGA ACAAACCTTT ACCTGTCCAA CACCAAGCCT TAACTCAGTT ACCATTAGGT
    CAGTCTGATG TGCATTCATC AGATCAAATG AGCACTGCAT TGCCTTCACA AGAGCCTTTG
    AACCAGGCCT CACATGTGCA GCCACAGCAT ATACCAGGAA CTGCACCTCA AGCCCAAAGT
    AATTCAGCAT TTCAACAGAT GCAACCTTTC TCAAGTCAAA CCCAGTCAAC ATGCATTGAG
    CAACCAACAT CTCTTACGCA TTTGCAGCAA CATCCCCCTG TTCAGGTAGC TGGGACAGAT
    AATCCCCAGT CATGTTCAGG ATCTATGCTT CAACAGATAA TTCCACCTGG GTATGCTTCG
    CAGCCAGTTT CACAACAGGT GTGGCCATCT TCAACTCCAG TCTTTTCAGA ATCTCTCTTA
    AAGCATCAGG ATGTTTACCA AAGTGCGGTG GCGGCACCAC AGACTCAATA CAAAGAACCA
    GAGTCAGAGC AGAGTTTGCA GTCTGCATGC CAAGCAGGGG CACCTGAACA GCATGTGTTT
    CAGAAACAAG ATTCTCTTCA GAGTAATGCT CATGGTGACT GTGCCAAAGA CAGCCCAGGT
    GCACACGACA ACACAGCTGG AAATGGCAAG CAAGATAAAA TGAAACAACG GCGAGCTTCC
    TGCCCCCGGC CTGAAAAAAT AGCCAGATTT ATCCTTACTG TAATGCAGGT ATCAACCTCT
    GGAGATAATA TGGTTGAGTG CCAGCTGGAA ACTCATAACA ACAAGATGGT GACTTTTAAA
    TTTGATGCAG ATGGTGATGC TCCGGAAGAT ATTGCCCATT ATATGGTGGA AGATGACTTT
    GTGCTGGAAG TTGAGAAAGA GAAGTTTGTC GAGGAGCTTA GGAATATAGT TACACAGGCC
    CAGGAGATAC TGCGCAGCAT ACCCGCTGAG GAGAGAACTG AATTGATACA GTCAGAGTCT
    AACAGTCAGG CTGGATCCTC AGATCATGTA CAGATGGGAG TCCCAACATT CCAGCAAACG
    GGGGGTGAAA CGGTTCCACA GTCCTCCCCA GTTGGGCGCT GGAGGTTTTT CATCAATCAG
    ACCATAAGGA ACCGCGAATC TCAGACCTCC TCTGTTTCAC AGTCTGCTGT GATCAGGGTT
    CCTCAATTAG CCACATTAGA TAATGAACAA GCAAGTCTCC CTAAGGATTC TTCAGCAGAC
    AGCTGTTCTC TTAAATCTGA GCATACAGTG CCGACTGTTC TTCTTCAGCC AACAGATGGT
    CATGCTTTCC CTACTGAGAC TGAACCTTTG ACTCTCCCTA CTTATGTGAC AGTTGGAAGT
    GAAATGTTAT CATCTCCATT GGACTGTAAT GCCTCAACTT TAGGTCAGAT TCAGCTAGAA
    CCTAGAAACT GTGGTAGTAA TATACTTGTG CTAGCAGAGA GCCAGCAGCC AGAAATACCT
    TCATCTTTTG ATCAGGACGT CCAAGGAGCA AATGGCCTGG TTAACACAAC TGAGACCAGT
    ACTGGACACT TACCGGCACA AAGCACTACA GGCTTGTTAC TTGGCTTAAC TATGTCAGAT
    GCTCTTCATC CAGTTGATAC AACTGTGTTG CCCCAGACTC CCTTGTCAGA AGTTTCCTTA
    GCGCACTCTG CATCAGTGCA AGAGTCTGAC ACTGAAGGAC CCCCTAAAAT AGACTATGTG
    GATAATCACA TCAAAACGTT GGATGAGAAA TTAAGAACTT TGTTATATCA GGACAGCTCT
    GCCAGCTCTT ATGCTGACAG CCAAAAGGAA ACACAGAGTA TAGAATCTCC CTTATCTTCA
    TCTGCTGAGG ATACTTTGTC GTGTCCTGTC CATGAAGCCC TCGATGTGAA TACTGCCGGT
    GTGCAGGCCA CTCCAGAGGA GGCAGACGCT AATGACCCTC CAGCGCAAAG AGATCCTGGA
    GAAGTCATCA CAGTACCTGG TACACTAACA CCATCAGAGA GTTCAGAAGA TGCCTGGGTT
    CCTGGAAGTT CCTCCCACGC TTGTTCCAAG CGCTCTATGG GTACTGGAGC CACGCATCTG
    CAATCAGGAG GTGGATATTT TGGCCTAAGC TTTACTTGTC CTAGTCTCAA AAATCCCATT
    AGCAAGAAAC CGTGGGCTCG CAAATTAAAA AGCTGGGCAT GCAGATTGCG GCATTCCAAC
    AGCTTATTCA AGAGGTCAAG AGTCCGGCAA GTTGAAGAGG AGGGAGTGAG ACTGGACATA
    TCTGATGCCA CACAAAGAGT TGTTGAAGCA TTTGCTGAAT GTGCCTTGTC AGAAGGGTCG
    CCATCAAAAA ACGGCACCTT CAAGCGTGGC CGTTTCCAGG TTGTTTCCGT TCCACAGCAA
    GAGCAGCCCA CTGCAAATGA ATCAGCTGGT GCTCCAGATA CCAAATACCC AGGTAACTCT
    CTTTTACAAG AAACTGGATC CGCAAATACA AAAGCAGAAG ACACTCCGCA GTCTGCCAGC
    ACAGCATGTG AGACTGATGC CTCCTCCCTC ACACCTGATA GAGAACTGGA TGAAACCTCA
    GCCAATGGCA GCAGTGTTCA GTCAAGTTCA GCCTTGTGGA TGAAAGATAT CAAGAAGCCG
    AGCAGCTGTA ATAAACAGCT AAGCAGTGAT TCTGAACAAT CTACTACTCC GGACAGGCCC
    CTGGAATTTA AAGGGAGAGA AAAAGATGCG GGGCAGCAAC CCCAGGGAGA GAAAATGTAT
    AACCAGAAAC AGAATTCCTT CTTTCACTCT CCATCTTCCC CCATGAGCAG TGATGACGAG
    TCCGAGTTAG AAGATGAAGA TTTGAAAGTG GAACTTCAGA AGCTCCGAGA AAAGCACATT
    CAAGAGGTGG TGACCCTTCA AACCCAGCAA AACCACGAGC TCCAGGAGCT GTATGTACGC
    TTGAAGTCTT TAAGGGAAAG CAAAGCACAG TCTTCAGATA CCTCTTCCCA GCCATTATCC
    CCCCGTCGCC CAAGGTCTCT AAAGAGTAAA CTGCGCAGCA GGCCCCAGTC CCTCACTCAC
    ATGGACAATG GGATCGGGCT TTCAGACCAC CAGTGCAGCG AGAGCAATTC CGATGCTTGC
    CAGCAGTCCT TGTCTGAGAA GAAGAGCATG TTCACGGATG ACTTTCATAA GCTGGTGGAT
    GACTGGGCAA AGGAAAATGC TGGCAACCAA GTTTTGAAAC CCAGTCTGAA TCAGATCAAG
    CAGAATCAAA GTCGCCTAGA GTCTGATAAC TGGGGTCGGA CCTATGAGAG TATGACAGCC
    ACATCAGGCT ATCCACCTGC ATGGATCCCT CAGATTCATG GCTCTTTGCC AGCTGCTGTC
    TCTCAGTCTC TCGTGCCCAC CAACTTTACA GCTGGAGCGA TGCCAGCATA CCAAGTGCCA
    CCAGCCTGCC AATTCACTTC AGTGAGCAGT AGTGGTTATC CTGTACCATG GTCTGCGCAA
    GCACCAGTAG TCCCTCCCCA GCATCTCACA GCATACCAGC CTGGCGTCGG AGTTCAAACT
    TTTGCAACCT CAACAGCTCA GAAGGCAACT ACAATCCCAA CATCTCCAAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031746230.1
    CDS273..8846
    Translation

    Target ORF information:

    RefSeq Version XM_031890370.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis WNK lysine deficient protein kinase 3 (wnk3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031890370.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCTACGG ATTCCGGAGA GCCAGCAAGT ACGGATGAAT CTGACAAAGC AGCTGGCTTT 
    TCATTACAAA ATAATCTGTG CTCCCAGGAA GGTTCCTTAA CCAAACAGGC AAGCATGGAG
    GAAAGTGACA AACCTTGCTC ATCTGCAGGG ACCAGTGAAA AGAAACGTTT CTTTAGAAAA
    AGCGTGGACA TTACAGAGGA TGACAGGATG TTGGAGCAGT CTCTAAAAGA TGAGAAAAGC
    ACTGATCCTC TGAGCCCACA GCTGATGGAG GCACATTCAA CAAAGGCTGC TGGTGCAGCA
    CAAGAAGGGA AAAATGATGC CAAAGCTAAT TCAGAGGCCT GTAAAGACTC TCCTAGAGAG
    AAAACTGAAA GGGAGATGGA GGAAGAGGCT GAAATGAAAG CCGTGGCTAC CTCTCCAAGT
    GGCAGATTTC TAAAGTTTGA CATTGAATTG GGCCGAGGGG CCTTTAAGAC AGTCTTCAAA
    GGACTTGACA CTGAAACTTG GGTAGAAGTT GCCTGGTGTG AGCTGCAGGA CAGGAAGTTA
    ACCAAAGCAG AGCAGCAACG ATTTAAAGAA GAGGCCGAGA TGTTAAAGGG CCTTCAGCAC
    CCAAATATTG TGCGGTTTTA TGATTCCTGG GAGTCTTCGC TGAAGGGAAA AAAATGTATA
    GTTTTGGTCA CAGAACTTAT GACATCTGGC ACTTTGAAAA CTTATTTGAA AAGATTTAAG
    GTAATGAAGC CAAAGGTGTT ACGGAGCTGG TGCCGGCAGA TCTTGAAGGG ACTTCAGTTC
    CTGCACACCA GGACACCTCC TATAATCCAT CGTGATCTGA AATGCGACAA CATTTTCATC
    ACTGGTCCCA CAGGCTCAGT GAAGATTGGG GACCTTGGTC TGGCCACCCT GATGCGTACA
    TCATTTGCAA AGAGTGTAAT TGGAACACCA GAATTCATGG CCCCAGAAAT GTATGAAGAG
    CATTACGACG AGTCAGTGGA TGTCTATGCA TTTGGCATGT GCATGCTGGA AATGGCTACA
    TCAGAATACC CATACTCAGA ATGCCAGAAT GCAGCTCAGA TCTACCGCAA AGTAACCAGC
    GGAATCAAAC CTGCCAGCTT CAATAAGGTG TCTGATCCTG AGGTAAAGGA AATAATTGAA
    AGCTGCATCC GTCAGAACAA GGCTGAAAGG TTATCGATAA AAGAACTTCT AAATCATGCT
    TTCTTTGCTG AAGACACTGG CTTGCGAGTT GAACTTGCTG AAGAAGATCA TTGTACAGAT
    TCATCTCTGG CCTTGAGACT TTGGGTGGAG GATCCAAAAA AGCTAAAAGG AAAACATAAA
    GATAATGAAG CTATTGAGTT CAGTTTTAAC TTAGACACGG ACAACCCTGA TGAAGTGGCA
    TGTGAGATGG TGAAATCTGG ATTTTTTCAT GAAAGTGACA GTAAGGCTGT GTCCAAATCT
    ATACGAGACA GAGTGTGTCT TATCAAGAAA ACAAGAGAGC GGAGGATACT GACGGGGTAT
    TCTGAGGACC GGCGTGATTC GCAAAGCAGA ATTGGAGGGG CAACACCTGC TCAGGTAGCA
    GTTCCCATTC CTGCAGTGTA CCAACAACCA GCTGGACACG AGAGTGAAGA AACGGAGGTA
    GACCAGCATG TACGCCAGCA ACTACTGCAA CAGCAGCAGC TTCAGCAGTG CTCTACAGTC
    ACAGTGTGCT CAACAGTCCC TGCTGACAGT CATTCGGAAG CAGGGGCAGG ATCTGTCATG
    CATTCGGATA CTACAAGCCA GCACAGTGCA GTATATGCTA TGAGCCATGA ACAGCTGAGT
    TGCCAGCAAG TGTCTGGGGT GCCTCAGGTA GAAAGTAGTC ATGTGGGGCA AGTCTACCAG
    TCCCAGCAAG TAGTGGGGCA GTATCAACAA ACACAGCAGG TTACTCAGCC GCAAATGCTT
    CCTGCACAAT GCCTTGCTCA GCCTGTCTCA TCTTATCTTC AGTCAGCAGC GGAAGGGCAG
    CTTGGCACAC AGTGCCTGGA TGCGAAGATG ACGTTTGCTG CAAATGGGAA TACTCTTTCG
    CAGTGTAACG CTTTAGATCC TTCAGTGATG TCAGTATTGC AGTCATCCTT TTCTTCGTTG
    TCCCCTAAAA TAATACCTGG ACAAATGACA TCACAGCAAG CAGGGGCTGT TATGCAGCTG
    GATGCTACTA TGACTGGAAT ACAAATTGTT ACACCTGCTG CAACGGACAC CCAAGTTCCT
    GCCCTTCTTC CTGTCTGCCA CCCAGCTTTA CTGGGTCAAT ACAGCAGCAA CATTCAGAAT
    GTGCTGCATC CATCTCAGAC TGTCCATGGG GCACCAGTTG GTCCTCAGCA GACGCAGCAC
    CCTGGAGCAT CTGTGCAGCA ACCAATAATT CCTACTTCAG ATACTTTAAA TTATTCAGCA
    ACGCTTCAGC CCAATATCCA GATACCATCT CACATAGAGC ATCTGCAAAT CTCTCAGCAG
    TCTAGTTACC ATCCTTCACT GCCTCCACAG CAACTTATAT TGCAGCCTGC ACTCACAGAA
    CAACCCCGAA ATCAAAACTT TTCTGATGCT GTTAAAGTTG AAGCTGTCCC ACAACAACTA
    CACATGGAAC CCCATTCAGA ACAGTTAGTA TTCAATGCAA AGACCTCGTC TCTGCATGGT
    GAGATGACAT CATTTGAACC ATCTGGGCAT TTACAGCAGT CTCTCACCTT GAATTCCTTG
    AATGTGCGTG AACAGTCTTC ATATGCCCAG CCTCCATTAC CTCACCTCGG AGTGCAACAA
    CTTTCCAGAG ATGTGGAACA AGTAGCAAAA ACAGTCATGA ACAATCCTCC AGAACAACCA
    CTATACATTC AGATTCCCCC ACTGGACACA TATGGACAAC AGTCTGTTAT TGGTTCTGAT
    CAGATGATAT ATACTCAGAA AGTTATTCCA TCTGATCAGC CTTTATTTTT AGAAAGTACT
    TCCCACTTGA CTAATCAGTC AGTGTATACC CAACAGAGTA TGCAAGCTTC AAATCAACAT
    AATTTTGTGC AACAAGCCAT ACAAACATCT GAGCAGTTGA TATATGCCCA CCCTGCTCTA
    GCGACTCCTG ATCAGACTGT GTACACTGAA AAAAATGCAC CATCAGCAGA ACAGCCAATA
    TTTGTACAGC AGGTTGTCTC GTCTGAACAA CCAGCATACA CACAACAGGT TATATCTGCT
    GAGCAGAAAG GCACACAGCC AGCTATTCCT GCAGAGCAGC CATTGTACAC CCAACAAACT
    GTTCCAGCTG AGCAGCCAGT GTATACTCAG CAAGTTATTC AATCGGAACA GCCAGCTCAC
    GAACAGCAAG GTATACTTGC TCAGCAGCCA GTGTATGCAC AGCAAGGTAT TCCAACTCAA
    CCAGTGTACA TACAGCAAAA TTTTGCAGCT CAGCACCAAG TGTACACACA ACAAAGCAAT
    TCCGCTGAGA CACCAATATA CACACAAAAG AATATTCAAA CTGACCAAGC AGTTTACACA
    CAGCAAGCTG TCGTTAAAAC TGAACAAACA GGATTCACCC AGCATGCTGA GCAAAAAGTG
    CTTGTACTCC AAGCAGACCA GACGATATAC GCACCGCAAC AGCCAATCCA AGTACAGCAA
    GCCGTTCATC TAAAAGAGCA GCCATATTAT GCACAGCAAG CAGCAGCTCA CATTCAGCAG
    TCTGCATCTG CTGAGCAAAC ACAATATCCA CAGTCTGCTT TATCTGCCGA GCAGTCCGTA
    TACTCACAGC AAACTGTATC TGCTGGGCAG TCTGGATATC CACAGCAAGC TTTGTCTGCC
    AAACAACCGC TATATATCCA GCAAGCAGTG TCTGCTGGGC AGCCAGTATA TCCACATCTG
    TCTGTATCCA CTGAGCAGCC ACAATATACA CAACACATTG TTCCCTCTGA TAAACAAATA
    TATACACAGC AAACTGTACC TGCTGAGCAG AGAGTGTACC CTCAGCATAT CGTATCAACT
    GAACAACCAA TGTATTCACA ACAAGTTCTA TCAGCTGAGC GTCCAGCATA TACACATCAA
    GTTGCAGCAC ATCCTGAACG CCTGGTATGC ATCCAACAAG TCCTTCAGCC CACAGAACAC
    CCTGCATATG CTTTACAGTC TATGCCATCC TCAGACAGAC CTGTATATCA ACAAGAATCT
    GCTTTGCTTT CTGGCCATAT GAACACCCAG CCAACTATTC CAGCTCATAC TCAAATTCTG
    TACACACAAG AGGCATTTAT GCCAACAGAA CAGCAGCCAT ATAACCTACA AACTGACAGC
    CCAATATATA TCCAACCAGT TGCTGGTGCT TCTCATCAGG TACCTGAACA ATCGGCCCCT
    ATTGAATCAG TATATAAACA GTCAGCTTCA CAGATGTCAT ATCCTCAGCA TGCAGTATCT
    TCATCTGATA CACAAATGTA TGCAAGCAAG ACGGTGGCTG CAAGTGCTCA GCTTGTGTAT
    GCTGTTCCAC CAGCAGAACA GGTGTACATT CAGCAGACAG GTTCTCTTTC TGAGCAACAA
    CAGTATGCCA GTCAAGCTGC TGTTCAGTCT GACAAGTCAG TTTATAGCTC TCAAACTATA
    CCCCCAGCAC AACAAGCTAT TTATACACAA GCACATCATG CTGTAATATC TGCAGAAAAG
    CCAGTGTTGC ACTCCCAAAA TGTGGAATCT GTTCCTCAGC AGACCTCTTT AAATGCTTCA
    GATGGTCTGT GGTGTATTCA GAAGTCACTG CCTCCTTCAG AGCAGATAGC TTACACTCTG
    CATCCTGCAC CATCTGGAGT GCCATCAGTT TGTGCTCAGC AGATGGTTCC CCTTGAACAG
    CTGGTAAACC CAAATTTGCC ATCTAATATT ATGCCACCTC AAGAGTTTAG GTCAGAGCAG
    CTTACTGAGA GTCAGTTGTA TGCACAACAA CACATTCAGT CTCCAAGACC ACTTCCATCA
    ATAATGCAGT CACAAGAGCA TGGGGCAAGT CACACACCCC CTCCTTTACA GACTGCAGAC
    AATCAAAGCT ATGCAAATTC CTTTGGTAAT CAACAAGGAG GTCAAGTGTT GCTACAGCCA
    GATCTTTTGA ACAAACCTTT ACCTGTCCAA CACCAAGCCT TAACTCAGTT ACCATTAGGT
    CAGTCTGATG TGCATTCATC AGATCAAATG AGCACTGCAT TGCCTTCACA AGAGCCTTTG
    AACCAGGCCT CACATGTGCA GCCACAGCAT ATACCAGGAA CTGCACCTCA AGCCCAAAGT
    AATTCAGCAT TTCAACAGAT GCAACCTTTC TCAAGTCAAA CCCAGTCAAC ATGCATTGAG
    CAACCAACAT CTCTTACGCA TTTGCAGCAA CATCCCCCTG TTCAGGTAGC TGGGACAGAT
    AATCCCCAGT CATGTTCAGG ATCTATGCTT CAACAGATAA TTCCACCTGG GTATGCTTCG
    CAGCCAGTTT CACAACAGGT GTGGCCATCT TCAACTCCAG TCTTTTCAGA ATCTCTCTTA
    AAGCATCAGG ATGTTTACCA AAGTGCGGTG GCGGCACCAC AGACTCAATA CAAAGAACCA
    GAGTCAGAGC AGAGTTTGCA GTCTGCATGC CAAGCAGGGG CACCTGAACA GCATGTGTTT
    CAGAAACAAG ATTCTCTTCA GAGTAATGCT CATGGTGACT GTGCCAAAGA CAGCCCAGGT
    GCACACGACA ACACAGCTGG AAATGGCAAG CAAGATAAAA TGAAACAACG GCGAGCTTCC
    TGCCCCCGGC CTGAAAAAAT AGCCAGATTT ATCCTTACTG TAATGCAGGT ATCAACCTCT
    GGAGATAATA TGGTTGAGTG CCAGCTGGAA ACTCATAACA ACAAGATGGT GACTTTTAAA
    TTTGATGCAG ATGGTGATGC TCCGGAAGAT ATTGCCCATT ATATGGTGGA AGATGACTTT
    GTGCTGGAAG TTGAGAAAGA GAAGTTTGTC GAGGAGCTTA GGAATATAGT TACACAGGCC
    CAGGAGATAC TGCGCAGCAT ACCCGCTGAG GAGAGAACTG AATTGATACA GTCAGAGTCT
    AACAGTCAGG CTGGATCCTC AGATCATGTA CAGATGGGAG TCCCAACATT CCAGCAAACG
    GGGGGTGAAA CGGTTCCACA GTCCTCCCCA GTTGGGCGCT GGAGGTTTTT CATCAATCAG
    ACCATAAGGA ACCGCGAATC TCAGACCTCC TCTGTTTCAC AGTCTGCTGT GATCAGGGTT
    CCTCAATTAG CCACATTAGA TAATGAACAA GCAAGTCTCC CTAAGGATTC TTCAGCAGAC
    AGCTGTTCTC TTAAATCTGA GCATACAGTG CCGACTGTTC TTCTTCAGCC AACAGATGGT
    CATGCTTTCC CTACTGAGAC TGAACCTTTG ACTCTCCCTA CTTATGTGAC AGTTGGAAGT
    GAAATGTTAT CATCTCCATT GGACTGTAAT GCCTCAACTT TAGGTCAGAT TCAGCTAGAA
    CCTAGAAACT GTGGTAGTAA TATACTTGTG CTAGCAGAGA GCCAGCAGCC AGAAATACCT
    TCATCTTTTG ATCAGGACGT CCAAGGAGCA AATGGCCTGG TTAACACAAC TGAGACCAGT
    ACTGGACACT TACCGGCACA AAGCACTACA GGCTTGTTAC TTGGCTTAAC TATGTCAGAT
    GCTCTTCATC CAGTTGATAC AACTGTGTTG CCCCAGACTC CCTTGTCAGA AGTTTCCTTA
    GCGCACTCTG CATCAGTGCA AGAGTCTGAC ACTGAAGGAC CCCCTAAAAT AGACTATGTG
    GATAATCACA TCAAAACGTT GGATGAGAAA TTAAGAACTT TGTTATATCA GGACAGCTCT
    GCCAGCTCTT ATGCTGACAG CCAAAAGGAA ACACAGAGTA TAGAATCTCC CTTATCTTCA
    TCTGCTGAGG ATACTTTGTC GTGTCCTGTC CATGAAGCCC TCGATGTGAA TACTGCCGGT
    GTGCAGGCCA CTCCAGAGGA GGCAGACGCT AATGACCCTC CAGCGCAAAG AGATCCTGGA
    GAAGTCATCA CAGTACCTGG TACACTAACA CCATCAGAGA GTTCAGAAGA TGCCTGGGTT
    CCTGGAAGTT CCTCCCACGC TTGTTCCAAG CGCTCTATGG GTACTGGAGC CACGCATCTG
    CAATCAGGAG GTGGATATTT TGGCCTAAGC TTTACTTGTC CTAGTCTCAA AAATCCCATT
    AGCAAGAAAC CGTGGGCTCG CAAATTAAAA AGCTGGGCAT GCAGATTGCG GCATTCCAAC
    AGCTTATTCA AGAGGTCAAG AGTCCGGCAA GTTGAAGAGG AGGGAGTGAG ACTGGACATA
    TCTGATGCCA CACAAAGAGT TGTTGAAGCA TTTGCTGAAT GTGCCTTGTC AGAAGGGTCG
    CCATCAAAAA ACGGCACCTT CAAGCGTGGC CGTTTCCAGG TTGTTTCCGT TCCACAGCAA
    GAGCAGCCCA CTGCAAATGA ATCAGCTGGT GCTCCAGATA CCAAATACCC AGGTAACTCT
    CTTTTACAAG AAACTGGATC CGCAAATACA AAAGCAGAAG ACACTCCGCA GTCTGCCAGC
    ACAGCATGTG AGACTGATGC CTCCTCCCTC ACACCTGATA GAGAACTGGA TGAAACCTCA
    GCCAATGGCA GCAGTGTTCA GTCAAGTTCA GCCTTGTGGA TGAAAGATAT CAAGAAGCCG
    AGCAGCTGTA ATAAACAGCT AAGCAGTGAT TCTGAACAAT CTACTACTCC GGACAGGCCC
    CTGGAATTTA AAGGGAGAGA AAAAGATGCG GGGCAGCAAC CCCAGGGAGA GAAAATGTAT
    AACCAGAAAC AGAATTCCTT CTTTCACTCT CCATCTTCCC CCATGAGCAG TGATGACGAG
    TCCGAGTTAG AAGATGAAGA TTTGAAAGTG GAACTTCAGA AGCTCCGAGA AAAGCACATT
    CAAGAGGTGG TGACCCTTCA AACCCAGCAA AACCACGAGC TCCAGGAGCT GTATGTACGC
    TTGAAGTCTT TAAGGGAAAG CAAAGCACAG TCTTCAGATA CCTCTTCCCA GCCATTATCC
    CCCCGTCGCC CAAGGTCTCT AAAGAGTAAA CTGCGCAGCA GGCCCCAGTC CCTCACTCAC
    ATGGACAATG GGATCGGGCT TTCAGACCAC CAGTGCAGCG AGAGCAATTC CGATGCTTGC
    CAGCAGTCCT TGTCTGAGAA GAAGAGCATG TTCACGGATG ACTTTCATAA GCTGGTGGAT
    GACTGGGCAA AGGAAAATGC TGGCAACCAA GTTTTGAAAC CCAGTCTGAA TCAGATCAAG
    CAGAATCAAA GTCGCCTAGA GTCTGATAAC TGGGGTCGGA CCTATGAGAG TATGACAGCC
    ACATCAGGCT ATCCACCTGC ATGGATCCCT CAGATTCATG GCTCTTTGCC AGCTGCTGTC
    TCTCAGTCTC TCGTGCCCAC CAACTTTACA GCTGGAGCGA TGCCAGCATA CCAAGTGCCA
    CCAGCCTGCC AATTCACTTC AGTGAGCAGT AGTGGTTATC CTGTACCATG GTCTGCGCAA
    GCACCAGTAG TCCCTCCCCA GCATCTCACA GCATACCAGC CTGGCGTCGG AGTTCAAACT
    TTTGCAACCT CAACAGCTCA GAAGGCAACT ACAATCCCAA CATCTCCAAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa63622
    Clone ID Related Accession (Same CDS sequence) XM_031890370.1 , XM_031890369.1 , XM_031890371.1 , XM_031890372.1
    Accession Version XM_031890369.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8574bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product serine/threonine-protein kinase WNK3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCTACGG ATTCCGGAGA GCCAGCAAGT ACGGATGAAT CTGACAAAGC AGCTGGCTTT 
    TCATTACAAA ATAATCTGTG CTCCCAGGAA GGTTCCTTAA CCAAACAGGC AAGCATGGAG
    GAAAGTGACA AACCTTGCTC ATCTGCAGGG ACCAGTGAAA AGAAACGTTT CTTTAGAAAA
    AGCGTGGACA TTACAGAGGA TGACAGGATG TTGGAGCAGT CTCTAAAAGA TGAGAAAAGC
    ACTGATCCTC TGAGCCCACA GCTGATGGAG GCACATTCAA CAAAGGCTGC TGGTGCAGCA
    CAAGAAGGGA AAAATGATGC CAAAGCTAAT TCAGAGGCCT GTAAAGACTC TCCTAGAGAG
    AAAACTGAAA GGGAGATGGA GGAAGAGGCT GAAATGAAAG CCGTGGCTAC CTCTCCAAGT
    GGCAGATTTC TAAAGTTTGA CATTGAATTG GGCCGAGGGG CCTTTAAGAC AGTCTTCAAA
    GGACTTGACA CTGAAACTTG GGTAGAAGTT GCCTGGTGTG AGCTGCAGGA CAGGAAGTTA
    ACCAAAGCAG AGCAGCAACG ATTTAAAGAA GAGGCCGAGA TGTTAAAGGG CCTTCAGCAC
    CCAAATATTG TGCGGTTTTA TGATTCCTGG GAGTCTTCGC TGAAGGGAAA AAAATGTATA
    GTTTTGGTCA CAGAACTTAT GACATCTGGC ACTTTGAAAA CTTATTTGAA AAGATTTAAG
    GTAATGAAGC CAAAGGTGTT ACGGAGCTGG TGCCGGCAGA TCTTGAAGGG ACTTCAGTTC
    CTGCACACCA GGACACCTCC TATAATCCAT CGTGATCTGA AATGCGACAA CATTTTCATC
    ACTGGTCCCA CAGGCTCAGT GAAGATTGGG GACCTTGGTC TGGCCACCCT GATGCGTACA
    TCATTTGCAA AGAGTGTAAT TGGAACACCA GAATTCATGG CCCCAGAAAT GTATGAAGAG
    CATTACGACG AGTCAGTGGA TGTCTATGCA TTTGGCATGT GCATGCTGGA AATGGCTACA
    TCAGAATACC CATACTCAGA ATGCCAGAAT GCAGCTCAGA TCTACCGCAA AGTAACCAGC
    GGAATCAAAC CTGCCAGCTT CAATAAGGTG TCTGATCCTG AGGTAAAGGA AATAATTGAA
    AGCTGCATCC GTCAGAACAA GGCTGAAAGG TTATCGATAA AAGAACTTCT AAATCATGCT
    TTCTTTGCTG AAGACACTGG CTTGCGAGTT GAACTTGCTG AAGAAGATCA TTGTACAGAT
    TCATCTCTGG CCTTGAGACT TTGGGTGGAG GATCCAAAAA AGCTAAAAGG AAAACATAAA
    GATAATGAAG CTATTGAGTT CAGTTTTAAC TTAGACACGG ACAACCCTGA TGAAGTGGCA
    TGTGAGATGG TGAAATCTGG ATTTTTTCAT GAAAGTGACA GTAAGGCTGT GTCCAAATCT
    ATACGAGACA GAGTGTGTCT TATCAAGAAA ACAAGAGAGC GGAGGATACT GACGGGGTAT
    TCTGAGGACC GGCGTGATTC GCAAAGCAGA ATTGGAGGGG CAACACCTGC TCAGGTAGCA
    GTTCCCATTC CTGCAGTGTA CCAACAACCA GCTGGACACG AGAGTGAAGA AACGGAGGTA
    GACCAGCATG TACGCCAGCA ACTACTGCAA CAGCAGCAGC TTCAGCAGTG CTCTACAGTC
    ACAGTGTGCT CAACAGTCCC TGCTGACAGT CATTCGGAAG CAGGGGCAGG ATCTGTCATG
    CATTCGGATA CTACAAGCCA GCACAGTGCA GTATATGCTA TGAGCCATGA ACAGCTGAGT
    TGCCAGCAAG TGTCTGGGGT GCCTCAGGTA GAAAGTAGTC ATGTGGGGCA AGTCTACCAG
    TCCCAGCAAG TAGTGGGGCA GTATCAACAA ACACAGCAGG TTACTCAGCC GCAAATGCTT
    CCTGCACAAT GCCTTGCTCA GCCTGTCTCA TCTTATCTTC AGTCAGCAGC GGAAGGGCAG
    CTTGGCACAC AGTGCCTGGA TGCGAAGATG ACGTTTGCTG CAAATGGGAA TACTCTTTCG
    CAGTGTAACG CTTTAGATCC TTCAGTGATG TCAGTATTGC AGTCATCCTT TTCTTCGTTG
    TCCCCTAAAA TAATACCTGG ACAAATGACA TCACAGCAAG CAGGGGCTGT TATGCAGCTG
    GATGCTACTA TGACTGGAAT ACAAATTGTT ACACCTGCTG CAACGGACAC CCAAGTTCCT
    GCCCTTCTTC CTGTCTGCCA CCCAGCTTTA CTGGGTCAAT ACAGCAGCAA CATTCAGAAT
    GTGCTGCATC CATCTCAGAC TGTCCATGGG GCACCAGTTG GTCCTCAGCA GACGCAGCAC
    CCTGGAGCAT CTGTGCAGCA ACCAATAATT CCTACTTCAG ATACTTTAAA TTATTCAGCA
    ACGCTTCAGC CCAATATCCA GATACCATCT CACATAGAGC ATCTGCAAAT CTCTCAGCAG
    TCTAGTTACC ATCCTTCACT GCCTCCACAG CAACTTATAT TGCAGCCTGC ACTCACAGAA
    CAACCCCGAA ATCAAAACTT TTCTGATGCT GTTAAAGTTG AAGCTGTCCC ACAACAACTA
    CACATGGAAC CCCATTCAGA ACAGTTAGTA TTCAATGCAA AGACCTCGTC TCTGCATGGT
    GAGATGACAT CATTTGAACC ATCTGGGCAT TTACAGCAGT CTCTCACCTT GAATTCCTTG
    AATGTGCGTG AACAGTCTTC ATATGCCCAG CCTCCATTAC CTCACCTCGG AGTGCAACAA
    CTTTCCAGAG ATGTGGAACA AGTAGCAAAA ACAGTCATGA ACAATCCTCC AGAACAACCA
    CTATACATTC AGATTCCCCC ACTGGACACA TATGGACAAC AGTCTGTTAT TGGTTCTGAT
    CAGATGATAT ATACTCAGAA AGTTATTCCA TCTGATCAGC CTTTATTTTT AGAAAGTACT
    TCCCACTTGA CTAATCAGTC AGTGTATACC CAACAGAGTA TGCAAGCTTC AAATCAACAT
    AATTTTGTGC AACAAGCCAT ACAAACATCT GAGCAGTTGA TATATGCCCA CCCTGCTCTA
    GCGACTCCTG ATCAGACTGT GTACACTGAA AAAAATGCAC CATCAGCAGA ACAGCCAATA
    TTTGTACAGC AGGTTGTCTC GTCTGAACAA CCAGCATACA CACAACAGGT TATATCTGCT
    GAGCAGAAAG GCACACAGCC AGCTATTCCT GCAGAGCAGC CATTGTACAC CCAACAAACT
    GTTCCAGCTG AGCAGCCAGT GTATACTCAG CAAGTTATTC AATCGGAACA GCCAGCTCAC
    GAACAGCAAG GTATACTTGC TCAGCAGCCA GTGTATGCAC AGCAAGGTAT TCCAACTCAA
    CCAGTGTACA TACAGCAAAA TTTTGCAGCT CAGCACCAAG TGTACACACA ACAAAGCAAT
    TCCGCTGAGA CACCAATATA CACACAAAAG AATATTCAAA CTGACCAAGC AGTTTACACA
    CAGCAAGCTG TCGTTAAAAC TGAACAAACA GGATTCACCC AGCATGCTGA GCAAAAAGTG
    CTTGTACTCC AAGCAGACCA GACGATATAC GCACCGCAAC AGCCAATCCA AGTACAGCAA
    GCCGTTCATC TAAAAGAGCA GCCATATTAT GCACAGCAAG CAGCAGCTCA CATTCAGCAG
    TCTGCATCTG CTGAGCAAAC ACAATATCCA CAGTCTGCTT TATCTGCCGA GCAGTCCGTA
    TACTCACAGC AAACTGTATC TGCTGGGCAG TCTGGATATC CACAGCAAGC TTTGTCTGCC
    AAACAACCGC TATATATCCA GCAAGCAGTG TCTGCTGGGC AGCCAGTATA TCCACATCTG
    TCTGTATCCA CTGAGCAGCC ACAATATACA CAACACATTG TTCCCTCTGA TAAACAAATA
    TATACACAGC AAACTGTACC TGCTGAGCAG AGAGTGTACC CTCAGCATAT CGTATCAACT
    GAACAACCAA TGTATTCACA ACAAGTTCTA TCAGCTGAGC GTCCAGCATA TACACATCAA
    GTTGCAGCAC ATCCTGAACG CCTGGTATGC ATCCAACAAG TCCTTCAGCC CACAGAACAC
    CCTGCATATG CTTTACAGTC TATGCCATCC TCAGACAGAC CTGTATATCA ACAAGAATCT
    GCTTTGCTTT CTGGCCATAT GAACACCCAG CCAACTATTC CAGCTCATAC TCAAATTCTG
    TACACACAAG AGGCATTTAT GCCAACAGAA CAGCAGCCAT ATAACCTACA AACTGACAGC
    CCAATATATA TCCAACCAGT TGCTGGTGCT TCTCATCAGG TACCTGAACA ATCGGCCCCT
    ATTGAATCAG TATATAAACA GTCAGCTTCA CAGATGTCAT ATCCTCAGCA TGCAGTATCT
    TCATCTGATA CACAAATGTA TGCAAGCAAG ACGGTGGCTG CAAGTGCTCA GCTTGTGTAT
    GCTGTTCCAC CAGCAGAACA GGTGTACATT CAGCAGACAG GTTCTCTTTC TGAGCAACAA
    CAGTATGCCA GTCAAGCTGC TGTTCAGTCT GACAAGTCAG TTTATAGCTC TCAAACTATA
    CCCCCAGCAC AACAAGCTAT TTATACACAA GCACATCATG CTGTAATATC TGCAGAAAAG
    CCAGTGTTGC ACTCCCAAAA TGTGGAATCT GTTCCTCAGC AGACCTCTTT AAATGCTTCA
    GATGGTCTGT GGTGTATTCA GAAGTCACTG CCTCCTTCAG AGCAGATAGC TTACACTCTG
    CATCCTGCAC CATCTGGAGT GCCATCAGTT TGTGCTCAGC AGATGGTTCC CCTTGAACAG
    CTGGTAAACC CAAATTTGCC ATCTAATATT ATGCCACCTC AAGAGTTTAG GTCAGAGCAG
    CTTACTGAGA GTCAGTTGTA TGCACAACAA CACATTCAGT CTCCAAGACC ACTTCCATCA
    ATAATGCAGT CACAAGAGCA TGGGGCAAGT CACACACCCC CTCCTTTACA GACTGCAGAC
    AATCAAAGCT ATGCAAATTC CTTTGGTAAT CAACAAGGAG GTCAAGTGTT GCTACAGCCA
    GATCTTTTGA ACAAACCTTT ACCTGTCCAA CACCAAGCCT TAACTCAGTT ACCATTAGGT
    CAGTCTGATG TGCATTCATC AGATCAAATG AGCACTGCAT TGCCTTCACA AGAGCCTTTG
    AACCAGGCCT CACATGTGCA GCCACAGCAT ATACCAGGAA CTGCACCTCA AGCCCAAAGT
    AATTCAGCAT TTCAACAGAT GCAACCTTTC TCAAGTCAAA CCCAGTCAAC ATGCATTGAG
    CAACCAACAT CTCTTACGCA TTTGCAGCAA CATCCCCCTG TTCAGGTAGC TGGGACAGAT
    AATCCCCAGT CATGTTCAGG ATCTATGCTT CAACAGATAA TTCCACCTGG GTATGCTTCG
    CAGCCAGTTT CACAACAGGT GTGGCCATCT TCAACTCCAG TCTTTTCAGA ATCTCTCTTA
    AAGCATCAGG ATGTTTACCA AAGTGCGGTG GCGGCACCAC AGACTCAATA CAAAGAACCA
    GAGTCAGAGC AGAGTTTGCA GTCTGCATGC CAAGCAGGGG CACCTGAACA GCATGTGTTT
    CAGAAACAAG ATTCTCTTCA GAGTAATGCT CATGGTGACT GTGCCAAAGA CAGCCCAGGT
    GCACACGACA ACACAGCTGG AAATGGCAAG CAAGATAAAA TGAAACAACG GCGAGCTTCC
    TGCCCCCGGC CTGAAAAAAT AGCCAGATTT ATCCTTACTG TAATGCAGGT ATCAACCTCT
    GGAGATAATA TGGTTGAGTG CCAGCTGGAA ACTCATAACA ACAAGATGGT GACTTTTAAA
    TTTGATGCAG ATGGTGATGC TCCGGAAGAT ATTGCCCATT ATATGGTGGA AGATGACTTT
    GTGCTGGAAG TTGAGAAAGA GAAGTTTGTC GAGGAGCTTA GGAATATAGT TACACAGGCC
    CAGGAGATAC TGCGCAGCAT ACCCGCTGAG GAGAGAACTG AATTGATACA GTCAGAGTCT
    AACAGTCAGG CTGGATCCTC AGATCATGTA CAGATGGGAG TCCCAACATT CCAGCAAACG
    GGGGGTGAAA CGGTTCCACA GTCCTCCCCA GTTGGGCGCT GGAGGTTTTT CATCAATCAG
    ACCATAAGGA ACCGCGAATC TCAGACCTCC TCTGTTTCAC AGTCTGCTGT GATCAGGGTT
    CCTCAATTAG CCACATTAGA TAATGAACAA GCAAGTCTCC CTAAGGATTC TTCAGCAGAC
    AGCTGTTCTC TTAAATCTGA GCATACAGTG CCGACTGTTC TTCTTCAGCC AACAGATGGT
    CATGCTTTCC CTACTGAGAC TGAACCTTTG ACTCTCCCTA CTTATGTGAC AGTTGGAAGT
    GAAATGTTAT CATCTCCATT GGACTGTAAT GCCTCAACTT TAGGTCAGAT TCAGCTAGAA
    CCTAGAAACT GTGGTAGTAA TATACTTGTG CTAGCAGAGA GCCAGCAGCC AGAAATACCT
    TCATCTTTTG ATCAGGACGT CCAAGGAGCA AATGGCCTGG TTAACACAAC TGAGACCAGT
    ACTGGACACT TACCGGCACA AAGCACTACA GGCTTGTTAC TTGGCTTAAC TATGTCAGAT
    GCTCTTCATC CAGTTGATAC AACTGTGTTG CCCCAGACTC CCTTGTCAGA AGTTTCCTTA
    GCGCACTCTG CATCAGTGCA AGAGTCTGAC ACTGAAGGAC CCCCTAAAAT AGACTATGTG
    GATAATCACA TCAAAACGTT GGATGAGAAA TTAAGAACTT TGTTATATCA GGACAGCTCT
    GCCAGCTCTT ATGCTGACAG CCAAAAGGAA ACACAGAGTA TAGAATCTCC CTTATCTTCA
    TCTGCTGAGG ATACTTTGTC GTGTCCTGTC CATGAAGCCC TCGATGTGAA TACTGCCGGT
    GTGCAGGCCA CTCCAGAGGA GGCAGACGCT AATGACCCTC CAGCGCAAAG AGATCCTGGA
    GAAGTCATCA CAGTACCTGG TACACTAACA CCATCAGAGA GTTCAGAAGA TGCCTGGGTT
    CCTGGAAGTT CCTCCCACGC TTGTTCCAAG CGCTCTATGG GTACTGGAGC CACGCATCTG
    CAATCAGGAG GTGGATATTT TGGCCTAAGC TTTACTTGTC CTAGTCTCAA AAATCCCATT
    AGCAAGAAAC CGTGGGCTCG CAAATTAAAA AGCTGGGCAT GCAGATTGCG GCATTCCAAC
    AGCTTATTCA AGAGGTCAAG AGTCCGGCAA GTTGAAGAGG AGGGAGTGAG ACTGGACATA
    TCTGATGCCA CACAAAGAGT TGTTGAAGCA TTTGCTGAAT GTGCCTTGTC AGAAGGGTCG
    CCATCAAAAA ACGGCACCTT CAAGCGTGGC CGTTTCCAGG TTGTTTCCGT TCCACAGCAA
    GAGCAGCCCA CTGCAAATGA ATCAGCTGGT GCTCCAGATA CCAAATACCC AGGTAACTCT
    CTTTTACAAG AAACTGGATC CGCAAATACA AAAGCAGAAG ACACTCCGCA GTCTGCCAGC
    ACAGCATGTG AGACTGATGC CTCCTCCCTC ACACCTGATA GAGAACTGGA TGAAACCTCA
    GCCAATGGCA GCAGTGTTCA GTCAAGTTCA GCCTTGTGGA TGAAAGATAT CAAGAAGCCG
    AGCAGCTGTA ATAAACAGCT AAGCAGTGAT TCTGAACAAT CTACTACTCC GGACAGGCCC
    CTGGAATTTA AAGGGAGAGA AAAAGATGCG GGGCAGCAAC CCCAGGGAGA GAAAATGTAT
    AACCAGAAAC AGAATTCCTT CTTTCACTCT CCATCTTCCC CCATGAGCAG TGATGACGAG
    TCCGAGTTAG AAGATGAAGA TTTGAAAGTG GAACTTCAGA AGCTCCGAGA AAAGCACATT
    CAAGAGGTGG TGACCCTTCA AACCCAGCAA AACCACGAGC TCCAGGAGCT GTATGTACGC
    TTGAAGTCTT TAAGGGAAAG CAAAGCACAG TCTTCAGATA CCTCTTCCCA GCCATTATCC
    CCCCGTCGCC CAAGGTCTCT AAAGAGTAAA CTGCGCAGCA GGCCCCAGTC CCTCACTCAC
    ATGGACAATG GGATCGGGCT TTCAGACCAC CAGTGCAGCG AGAGCAATTC CGATGCTTGC
    CAGCAGTCCT TGTCTGAGAA GAAGAGCATG TTCACGGATG ACTTTCATAA GCTGGTGGAT
    GACTGGGCAA AGGAAAATGC TGGCAACCAA GTTTTGAAAC CCAGTCTGAA TCAGATCAAG
    CAGAATCAAA GTCGCCTAGA GTCTGATAAC TGGGGTCGGA CCTATGAGAG TATGACAGCC
    ACATCAGGCT ATCCACCTGC ATGGATCCCT CAGATTCATG GCTCTTTGCC AGCTGCTGTC
    TCTCAGTCTC TCGTGCCCAC CAACTTTACA GCTGGAGCGA TGCCAGCATA CCAAGTGCCA
    CCAGCCTGCC AATTCACTTC AGTGAGCAGT AGTGGTTATC CTGTACCATG GTCTGCGCAA
    GCACCAGTAG TCCCTCCCCA GCATCTCACA GCATACCAGC CTGGCGTCGG AGTTCAAACT
    TTTGCAACCT CAACAGCTCA GAAGGCAACT ACAATCCCAA CATCTCCAAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031746229.1
    CDS193..8766
    Translation

    Target ORF information:

    RefSeq Version XM_031890369.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis WNK lysine deficient protein kinase 3 (wnk3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031890369.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCTACGG ATTCCGGAGA GCCAGCAAGT ACGGATGAAT CTGACAAAGC AGCTGGCTTT 
    TCATTACAAA ATAATCTGTG CTCCCAGGAA GGTTCCTTAA CCAAACAGGC AAGCATGGAG
    GAAAGTGACA AACCTTGCTC ATCTGCAGGG ACCAGTGAAA AGAAACGTTT CTTTAGAAAA
    AGCGTGGACA TTACAGAGGA TGACAGGATG TTGGAGCAGT CTCTAAAAGA TGAGAAAAGC
    ACTGATCCTC TGAGCCCACA GCTGATGGAG GCACATTCAA CAAAGGCTGC TGGTGCAGCA
    CAAGAAGGGA AAAATGATGC CAAAGCTAAT TCAGAGGCCT GTAAAGACTC TCCTAGAGAG
    AAAACTGAAA GGGAGATGGA GGAAGAGGCT GAAATGAAAG CCGTGGCTAC CTCTCCAAGT
    GGCAGATTTC TAAAGTTTGA CATTGAATTG GGCCGAGGGG CCTTTAAGAC AGTCTTCAAA
    GGACTTGACA CTGAAACTTG GGTAGAAGTT GCCTGGTGTG AGCTGCAGGA CAGGAAGTTA
    ACCAAAGCAG AGCAGCAACG ATTTAAAGAA GAGGCCGAGA TGTTAAAGGG CCTTCAGCAC
    CCAAATATTG TGCGGTTTTA TGATTCCTGG GAGTCTTCGC TGAAGGGAAA AAAATGTATA
    GTTTTGGTCA CAGAACTTAT GACATCTGGC ACTTTGAAAA CTTATTTGAA AAGATTTAAG
    GTAATGAAGC CAAAGGTGTT ACGGAGCTGG TGCCGGCAGA TCTTGAAGGG ACTTCAGTTC
    CTGCACACCA GGACACCTCC TATAATCCAT CGTGATCTGA AATGCGACAA CATTTTCATC
    ACTGGTCCCA CAGGCTCAGT GAAGATTGGG GACCTTGGTC TGGCCACCCT GATGCGTACA
    TCATTTGCAA AGAGTGTAAT TGGAACACCA GAATTCATGG CCCCAGAAAT GTATGAAGAG
    CATTACGACG AGTCAGTGGA TGTCTATGCA TTTGGCATGT GCATGCTGGA AATGGCTACA
    TCAGAATACC CATACTCAGA ATGCCAGAAT GCAGCTCAGA TCTACCGCAA AGTAACCAGC
    GGAATCAAAC CTGCCAGCTT CAATAAGGTG TCTGATCCTG AGGTAAAGGA AATAATTGAA
    AGCTGCATCC GTCAGAACAA GGCTGAAAGG TTATCGATAA AAGAACTTCT AAATCATGCT
    TTCTTTGCTG AAGACACTGG CTTGCGAGTT GAACTTGCTG AAGAAGATCA TTGTACAGAT
    TCATCTCTGG CCTTGAGACT TTGGGTGGAG GATCCAAAAA AGCTAAAAGG AAAACATAAA
    GATAATGAAG CTATTGAGTT CAGTTTTAAC TTAGACACGG ACAACCCTGA TGAAGTGGCA
    TGTGAGATGG TGAAATCTGG ATTTTTTCAT GAAAGTGACA GTAAGGCTGT GTCCAAATCT
    ATACGAGACA GAGTGTGTCT TATCAAGAAA ACAAGAGAGC GGAGGATACT GACGGGGTAT
    TCTGAGGACC GGCGTGATTC GCAAAGCAGA ATTGGAGGGG CAACACCTGC TCAGGTAGCA
    GTTCCCATTC CTGCAGTGTA CCAACAACCA GCTGGACACG AGAGTGAAGA AACGGAGGTA
    GACCAGCATG TACGCCAGCA ACTACTGCAA CAGCAGCAGC TTCAGCAGTG CTCTACAGTC
    ACAGTGTGCT CAACAGTCCC TGCTGACAGT CATTCGGAAG CAGGGGCAGG ATCTGTCATG
    CATTCGGATA CTACAAGCCA GCACAGTGCA GTATATGCTA TGAGCCATGA ACAGCTGAGT
    TGCCAGCAAG TGTCTGGGGT GCCTCAGGTA GAAAGTAGTC ATGTGGGGCA AGTCTACCAG
    TCCCAGCAAG TAGTGGGGCA GTATCAACAA ACACAGCAGG TTACTCAGCC GCAAATGCTT
    CCTGCACAAT GCCTTGCTCA GCCTGTCTCA TCTTATCTTC AGTCAGCAGC GGAAGGGCAG
    CTTGGCACAC AGTGCCTGGA TGCGAAGATG ACGTTTGCTG CAAATGGGAA TACTCTTTCG
    CAGTGTAACG CTTTAGATCC TTCAGTGATG TCAGTATTGC AGTCATCCTT TTCTTCGTTG
    TCCCCTAAAA TAATACCTGG ACAAATGACA TCACAGCAAG CAGGGGCTGT TATGCAGCTG
    GATGCTACTA TGACTGGAAT ACAAATTGTT ACACCTGCTG CAACGGACAC CCAAGTTCCT
    GCCCTTCTTC CTGTCTGCCA CCCAGCTTTA CTGGGTCAAT ACAGCAGCAA CATTCAGAAT
    GTGCTGCATC CATCTCAGAC TGTCCATGGG GCACCAGTTG GTCCTCAGCA GACGCAGCAC
    CCTGGAGCAT CTGTGCAGCA ACCAATAATT CCTACTTCAG ATACTTTAAA TTATTCAGCA
    ACGCTTCAGC CCAATATCCA GATACCATCT CACATAGAGC ATCTGCAAAT CTCTCAGCAG
    TCTAGTTACC ATCCTTCACT GCCTCCACAG CAACTTATAT TGCAGCCTGC ACTCACAGAA
    CAACCCCGAA ATCAAAACTT TTCTGATGCT GTTAAAGTTG AAGCTGTCCC ACAACAACTA
    CACATGGAAC CCCATTCAGA ACAGTTAGTA TTCAATGCAA AGACCTCGTC TCTGCATGGT
    GAGATGACAT CATTTGAACC ATCTGGGCAT TTACAGCAGT CTCTCACCTT GAATTCCTTG
    AATGTGCGTG AACAGTCTTC ATATGCCCAG CCTCCATTAC CTCACCTCGG AGTGCAACAA
    CTTTCCAGAG ATGTGGAACA AGTAGCAAAA ACAGTCATGA ACAATCCTCC AGAACAACCA
    CTATACATTC AGATTCCCCC ACTGGACACA TATGGACAAC AGTCTGTTAT TGGTTCTGAT
    CAGATGATAT ATACTCAGAA AGTTATTCCA TCTGATCAGC CTTTATTTTT AGAAAGTACT
    TCCCACTTGA CTAATCAGTC AGTGTATACC CAACAGAGTA TGCAAGCTTC AAATCAACAT
    AATTTTGTGC AACAAGCCAT ACAAACATCT GAGCAGTTGA TATATGCCCA CCCTGCTCTA
    GCGACTCCTG ATCAGACTGT GTACACTGAA AAAAATGCAC CATCAGCAGA ACAGCCAATA
    TTTGTACAGC AGGTTGTCTC GTCTGAACAA CCAGCATACA CACAACAGGT TATATCTGCT
    GAGCAGAAAG GCACACAGCC AGCTATTCCT GCAGAGCAGC CATTGTACAC CCAACAAACT
    GTTCCAGCTG AGCAGCCAGT GTATACTCAG CAAGTTATTC AATCGGAACA GCCAGCTCAC
    GAACAGCAAG GTATACTTGC TCAGCAGCCA GTGTATGCAC AGCAAGGTAT TCCAACTCAA
    CCAGTGTACA TACAGCAAAA TTTTGCAGCT CAGCACCAAG TGTACACACA ACAAAGCAAT
    TCCGCTGAGA CACCAATATA CACACAAAAG AATATTCAAA CTGACCAAGC AGTTTACACA
    CAGCAAGCTG TCGTTAAAAC TGAACAAACA GGATTCACCC AGCATGCTGA GCAAAAAGTG
    CTTGTACTCC AAGCAGACCA GACGATATAC GCACCGCAAC AGCCAATCCA AGTACAGCAA
    GCCGTTCATC TAAAAGAGCA GCCATATTAT GCACAGCAAG CAGCAGCTCA CATTCAGCAG
    TCTGCATCTG CTGAGCAAAC ACAATATCCA CAGTCTGCTT TATCTGCCGA GCAGTCCGTA
    TACTCACAGC AAACTGTATC TGCTGGGCAG TCTGGATATC CACAGCAAGC TTTGTCTGCC
    AAACAACCGC TATATATCCA GCAAGCAGTG TCTGCTGGGC AGCCAGTATA TCCACATCTG
    TCTGTATCCA CTGAGCAGCC ACAATATACA CAACACATTG TTCCCTCTGA TAAACAAATA
    TATACACAGC AAACTGTACC TGCTGAGCAG AGAGTGTACC CTCAGCATAT CGTATCAACT
    GAACAACCAA TGTATTCACA ACAAGTTCTA TCAGCTGAGC GTCCAGCATA TACACATCAA
    GTTGCAGCAC ATCCTGAACG CCTGGTATGC ATCCAACAAG TCCTTCAGCC CACAGAACAC
    CCTGCATATG CTTTACAGTC TATGCCATCC TCAGACAGAC CTGTATATCA ACAAGAATCT
    GCTTTGCTTT CTGGCCATAT GAACACCCAG CCAACTATTC CAGCTCATAC TCAAATTCTG
    TACACACAAG AGGCATTTAT GCCAACAGAA CAGCAGCCAT ATAACCTACA AACTGACAGC
    CCAATATATA TCCAACCAGT TGCTGGTGCT TCTCATCAGG TACCTGAACA ATCGGCCCCT
    ATTGAATCAG TATATAAACA GTCAGCTTCA CAGATGTCAT ATCCTCAGCA TGCAGTATCT
    TCATCTGATA CACAAATGTA TGCAAGCAAG ACGGTGGCTG CAAGTGCTCA GCTTGTGTAT
    GCTGTTCCAC CAGCAGAACA GGTGTACATT CAGCAGACAG GTTCTCTTTC TGAGCAACAA
    CAGTATGCCA GTCAAGCTGC TGTTCAGTCT GACAAGTCAG TTTATAGCTC TCAAACTATA
    CCCCCAGCAC AACAAGCTAT TTATACACAA GCACATCATG CTGTAATATC TGCAGAAAAG
    CCAGTGTTGC ACTCCCAAAA TGTGGAATCT GTTCCTCAGC AGACCTCTTT AAATGCTTCA
    GATGGTCTGT GGTGTATTCA GAAGTCACTG CCTCCTTCAG AGCAGATAGC TTACACTCTG
    CATCCTGCAC CATCTGGAGT GCCATCAGTT TGTGCTCAGC AGATGGTTCC CCTTGAACAG
    CTGGTAAACC CAAATTTGCC ATCTAATATT ATGCCACCTC AAGAGTTTAG GTCAGAGCAG
    CTTACTGAGA GTCAGTTGTA TGCACAACAA CACATTCAGT CTCCAAGACC ACTTCCATCA
    ATAATGCAGT CACAAGAGCA TGGGGCAAGT CACACACCCC CTCCTTTACA GACTGCAGAC
    AATCAAAGCT ATGCAAATTC CTTTGGTAAT CAACAAGGAG GTCAAGTGTT GCTACAGCCA
    GATCTTTTGA ACAAACCTTT ACCTGTCCAA CACCAAGCCT TAACTCAGTT ACCATTAGGT
    CAGTCTGATG TGCATTCATC AGATCAAATG AGCACTGCAT TGCCTTCACA AGAGCCTTTG
    AACCAGGCCT CACATGTGCA GCCACAGCAT ATACCAGGAA CTGCACCTCA AGCCCAAAGT
    AATTCAGCAT TTCAACAGAT GCAACCTTTC TCAAGTCAAA CCCAGTCAAC ATGCATTGAG
    CAACCAACAT CTCTTACGCA TTTGCAGCAA CATCCCCCTG TTCAGGTAGC TGGGACAGAT
    AATCCCCAGT CATGTTCAGG ATCTATGCTT CAACAGATAA TTCCACCTGG GTATGCTTCG
    CAGCCAGTTT CACAACAGGT GTGGCCATCT TCAACTCCAG TCTTTTCAGA ATCTCTCTTA
    AAGCATCAGG ATGTTTACCA AAGTGCGGTG GCGGCACCAC AGACTCAATA CAAAGAACCA
    GAGTCAGAGC AGAGTTTGCA GTCTGCATGC CAAGCAGGGG CACCTGAACA GCATGTGTTT
    CAGAAACAAG ATTCTCTTCA GAGTAATGCT CATGGTGACT GTGCCAAAGA CAGCCCAGGT
    GCACACGACA ACACAGCTGG AAATGGCAAG CAAGATAAAA TGAAACAACG GCGAGCTTCC
    TGCCCCCGGC CTGAAAAAAT AGCCAGATTT ATCCTTACTG TAATGCAGGT ATCAACCTCT
    GGAGATAATA TGGTTGAGTG CCAGCTGGAA ACTCATAACA ACAAGATGGT GACTTTTAAA
    TTTGATGCAG ATGGTGATGC TCCGGAAGAT ATTGCCCATT ATATGGTGGA AGATGACTTT
    GTGCTGGAAG TTGAGAAAGA GAAGTTTGTC GAGGAGCTTA GGAATATAGT TACACAGGCC
    CAGGAGATAC TGCGCAGCAT ACCCGCTGAG GAGAGAACTG AATTGATACA GTCAGAGTCT
    AACAGTCAGG CTGGATCCTC AGATCATGTA CAGATGGGAG TCCCAACATT CCAGCAAACG
    GGGGGTGAAA CGGTTCCACA GTCCTCCCCA GTTGGGCGCT GGAGGTTTTT CATCAATCAG
    ACCATAAGGA ACCGCGAATC TCAGACCTCC TCTGTTTCAC AGTCTGCTGT GATCAGGGTT
    CCTCAATTAG CCACATTAGA TAATGAACAA GCAAGTCTCC CTAAGGATTC TTCAGCAGAC
    AGCTGTTCTC TTAAATCTGA GCATACAGTG CCGACTGTTC TTCTTCAGCC AACAGATGGT
    CATGCTTTCC CTACTGAGAC TGAACCTTTG ACTCTCCCTA CTTATGTGAC AGTTGGAAGT
    GAAATGTTAT CATCTCCATT GGACTGTAAT GCCTCAACTT TAGGTCAGAT TCAGCTAGAA
    CCTAGAAACT GTGGTAGTAA TATACTTGTG CTAGCAGAGA GCCAGCAGCC AGAAATACCT
    TCATCTTTTG ATCAGGACGT CCAAGGAGCA AATGGCCTGG TTAACACAAC TGAGACCAGT
    ACTGGACACT TACCGGCACA AAGCACTACA GGCTTGTTAC TTGGCTTAAC TATGTCAGAT
    GCTCTTCATC CAGTTGATAC AACTGTGTTG CCCCAGACTC CCTTGTCAGA AGTTTCCTTA
    GCGCACTCTG CATCAGTGCA AGAGTCTGAC ACTGAAGGAC CCCCTAAAAT AGACTATGTG
    GATAATCACA TCAAAACGTT GGATGAGAAA TTAAGAACTT TGTTATATCA GGACAGCTCT
    GCCAGCTCTT ATGCTGACAG CCAAAAGGAA ACACAGAGTA TAGAATCTCC CTTATCTTCA
    TCTGCTGAGG ATACTTTGTC GTGTCCTGTC CATGAAGCCC TCGATGTGAA TACTGCCGGT
    GTGCAGGCCA CTCCAGAGGA GGCAGACGCT AATGACCCTC CAGCGCAAAG AGATCCTGGA
    GAAGTCATCA CAGTACCTGG TACACTAACA CCATCAGAGA GTTCAGAAGA TGCCTGGGTT
    CCTGGAAGTT CCTCCCACGC TTGTTCCAAG CGCTCTATGG GTACTGGAGC CACGCATCTG
    CAATCAGGAG GTGGATATTT TGGCCTAAGC TTTACTTGTC CTAGTCTCAA AAATCCCATT
    AGCAAGAAAC CGTGGGCTCG CAAATTAAAA AGCTGGGCAT GCAGATTGCG GCATTCCAAC
    AGCTTATTCA AGAGGTCAAG AGTCCGGCAA GTTGAAGAGG AGGGAGTGAG ACTGGACATA
    TCTGATGCCA CACAAAGAGT TGTTGAAGCA TTTGCTGAAT GTGCCTTGTC AGAAGGGTCG
    CCATCAAAAA ACGGCACCTT CAAGCGTGGC CGTTTCCAGG TTGTTTCCGT TCCACAGCAA
    GAGCAGCCCA CTGCAAATGA ATCAGCTGGT GCTCCAGATA CCAAATACCC AGGTAACTCT
    CTTTTACAAG AAACTGGATC CGCAAATACA AAAGCAGAAG ACACTCCGCA GTCTGCCAGC
    ACAGCATGTG AGACTGATGC CTCCTCCCTC ACACCTGATA GAGAACTGGA TGAAACCTCA
    GCCAATGGCA GCAGTGTTCA GTCAAGTTCA GCCTTGTGGA TGAAAGATAT CAAGAAGCCG
    AGCAGCTGTA ATAAACAGCT AAGCAGTGAT TCTGAACAAT CTACTACTCC GGACAGGCCC
    CTGGAATTTA AAGGGAGAGA AAAAGATGCG GGGCAGCAAC CCCAGGGAGA GAAAATGTAT
    AACCAGAAAC AGAATTCCTT CTTTCACTCT CCATCTTCCC CCATGAGCAG TGATGACGAG
    TCCGAGTTAG AAGATGAAGA TTTGAAAGTG GAACTTCAGA AGCTCCGAGA AAAGCACATT
    CAAGAGGTGG TGACCCTTCA AACCCAGCAA AACCACGAGC TCCAGGAGCT GTATGTACGC
    TTGAAGTCTT TAAGGGAAAG CAAAGCACAG TCTTCAGATA CCTCTTCCCA GCCATTATCC
    CCCCGTCGCC CAAGGTCTCT AAAGAGTAAA CTGCGCAGCA GGCCCCAGTC CCTCACTCAC
    ATGGACAATG GGATCGGGCT TTCAGACCAC CAGTGCAGCG AGAGCAATTC CGATGCTTGC
    CAGCAGTCCT TGTCTGAGAA GAAGAGCATG TTCACGGATG ACTTTCATAA GCTGGTGGAT
    GACTGGGCAA AGGAAAATGC TGGCAACCAA GTTTTGAAAC CCAGTCTGAA TCAGATCAAG
    CAGAATCAAA GTCGCCTAGA GTCTGATAAC TGGGGTCGGA CCTATGAGAG TATGACAGCC
    ACATCAGGCT ATCCACCTGC ATGGATCCCT CAGATTCATG GCTCTTTGCC AGCTGCTGTC
    TCTCAGTCTC TCGTGCCCAC CAACTTTACA GCTGGAGCGA TGCCAGCATA CCAAGTGCCA
    CCAGCCTGCC AATTCACTTC AGTGAGCAGT AGTGGTTATC CTGTACCATG GTCTGCGCAA
    GCACCAGTAG TCCCTCCCCA GCATCTCACA GCATACCAGC CTGGCGTCGG AGTTCAAACT
    TTTGCAACCT CAACAGCTCA GAAGGCAACT ACAATCCCAA CATCTCCAAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa63622
    Clone ID Related Accession (Same CDS sequence) XM_031890370.1 , XM_031890369.1 , XM_031890371.1 , XM_031890372.1
    Accession Version XM_031890371.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8574bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product serine/threonine-protein kinase WNK3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1321
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    8401
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    8521
    ATGGCTACGG ATTCCGGAGA GCCAGCAAGT ACGGATGAAT CTGACAAAGC AGCTGGCTTT 
    TCATTACAAA ATAATCTGTG CTCCCAGGAA GGTTCCTTAA CCAAACAGGC AAGCATGGAG
    GAAAGTGACA AACCTTGCTC ATCTGCAGGG ACCAGTGAAA AGAAACGTTT CTTTAGAAAA
    AGCGTGGACA TTACAGAGGA TGACAGGATG TTGGAGCAGT CTCTAAAAGA TGAGAAAAGC
    ACTGATCCTC TGAGCCCACA GCTGATGGAG GCACATTCAA CAAAGGCTGC TGGTGCAGCA
    CAAGAAGGGA AAAATGATGC CAAAGCTAAT TCAGAGGCCT GTAAAGACTC TCCTAGAGAG
    AAAACTGAAA GGGAGATGGA GGAAGAGGCT GAAATGAAAG CCGTGGCTAC CTCTCCAAGT
    GGCAGATTTC TAAAGTTTGA CATTGAATTG GGCCGAGGGG CCTTTAAGAC AGTCTTCAAA
    GGACTTGACA CTGAAACTTG GGTAGAAGTT GCCTGGTGTG AGCTGCAGGA CAGGAAGTTA
    ACCAAAGCAG AGCAGCAACG ATTTAAAGAA GAGGCCGAGA TGTTAAAGGG CCTTCAGCAC
    CCAAATATTG TGCGGTTTTA TGATTCCTGG GAGTCTTCGC TGAAGGGAAA AAAATGTATA
    GTTTTGGTCA CAGAACTTAT GACATCTGGC ACTTTGAAAA CTTATTTGAA AAGATTTAAG
    GTAATGAAGC CAAAGGTGTT ACGGAGCTGG TGCCGGCAGA TCTTGAAGGG ACTTCAGTTC
    CTGCACACCA GGACACCTCC TATAATCCAT CGTGATCTGA AATGCGACAA CATTTTCATC
    ACTGGTCCCA CAGGCTCAGT GAAGATTGGG GACCTTGGTC TGGCCACCCT GATGCGTACA
    TCATTTGCAA AGAGTGTAAT TGGAACACCA GAATTCATGG CCCCAGAAAT GTATGAAGAG
    CATTACGACG AGTCAGTGGA TGTCTATGCA TTTGGCATGT GCATGCTGGA AATGGCTACA
    TCAGAATACC CATACTCAGA ATGCCAGAAT GCAGCTCAGA TCTACCGCAA AGTAACCAGC
    GGAATCAAAC CTGCCAGCTT CAATAAGGTG TCTGATCCTG AGGTAAAGGA AATAATTGAA
    AGCTGCATCC GTCAGAACAA GGCTGAAAGG TTATCGATAA AAGAACTTCT AAATCATGCT
    TTCTTTGCTG AAGACACTGG CTTGCGAGTT GAACTTGCTG AAGAAGATCA TTGTACAGAT
    TCATCTCTGG CCTTGAGACT TTGGGTGGAG GATCCAAAAA AGCTAAAAGG AAAACATAAA
    GATAATGAAG CTATTGAGTT CAGTTTTAAC TTAGACACGG ACAACCCTGA TGAAGTGGCA
    TGTGAGATGG TGAAATCTGG ATTTTTTCAT GAAAGTGACA GTAAGGCTGT GTCCAAATCT
    ATACGAGACA GAGTGTGTCT TATCAAGAAA ACAAGAGAGC GGAGGATACT GACGGGGTAT
    TCTGAGGACC GGCGTGATTC GCAAAGCAGA ATTGGAGGGG CAACACCTGC TCAGGTAGCA
    GTTCCCATTC CTGCAGTGTA CCAACAACCA GCTGGACACG AGAGTGAAGA AACGGAGGTA
    GACCAGCATG TACGCCAGCA ACTACTGCAA CAGCAGCAGC TTCAGCAGTG CTCTACAGTC
    ACAGTGTGCT CAACAGTCCC TGCTGACAGT CATTCGGAAG CAGGGGCAGG ATCTGTCATG
    CATTCGGATA CTACAAGCCA GCACAGTGCA GTATATGCTA TGAGCCATGA ACAGCTGAGT
    TGCCAGCAAG TGTCTGGGGT GCCTCAGGTA GAAAGTAGTC ATGTGGGGCA AGTCTACCAG
    TCCCAGCAAG TAGTGGGGCA GTATCAACAA ACACAGCAGG TTACTCAGCC GCAAATGCTT
    CCTGCACAAT GCCTTGCTCA GCCTGTCTCA TCTTATCTTC AGTCAGCAGC GGAAGGGCAG
    CTTGGCACAC AGTGCCTGGA TGCGAAGATG ACGTTTGCTG CAAATGGGAA TACTCTTTCG
    CAGTGTAACG CTTTAGATCC TTCAGTGATG TCAGTATTGC AGTCATCCTT TTCTTCGTTG
    TCCCCTAAAA TAATACCTGG ACAAATGACA TCACAGCAAG CAGGGGCTGT TATGCAGCTG
    GATGCTACTA TGACTGGAAT ACAAATTGTT ACACCTGCTG CAACGGACAC CCAAGTTCCT
    GCCCTTCTTC CTGTCTGCCA CCCAGCTTTA CTGGGTCAAT ACAGCAGCAA CATTCAGAAT
    GTGCTGCATC CATCTCAGAC TGTCCATGGG GCACCAGTTG GTCCTCAGCA GACGCAGCAC
    CCTGGAGCAT CTGTGCAGCA ACCAATAATT CCTACTTCAG ATACTTTAAA TTATTCAGCA
    ACGCTTCAGC CCAATATCCA GATACCATCT CACATAGAGC ATCTGCAAAT CTCTCAGCAG
    TCTAGTTACC ATCCTTCACT GCCTCCACAG CAACTTATAT TGCAGCCTGC ACTCACAGAA
    CAACCCCGAA ATCAAAACTT TTCTGATGCT GTTAAAGTTG AAGCTGTCCC ACAACAACTA
    CACATGGAAC CCCATTCAGA ACAGTTAGTA TTCAATGCAA AGACCTCGTC TCTGCATGGT
    GAGATGACAT CATTTGAACC ATCTGGGCAT TTACAGCAGT CTCTCACCTT GAATTCCTTG
    AATGTGCGTG AACAGTCTTC ATATGCCCAG CCTCCATTAC CTCACCTCGG AGTGCAACAA
    CTTTCCAGAG ATGTGGAACA AGTAGCAAAA ACAGTCATGA ACAATCCTCC AGAACAACCA
    CTATACATTC AGATTCCCCC ACTGGACACA TATGGACAAC AGTCTGTTAT TGGTTCTGAT
    CAGATGATAT ATACTCAGAA AGTTATTCCA TCTGATCAGC CTTTATTTTT AGAAAGTACT
    TCCCACTTGA CTAATCAGTC AGTGTATACC CAACAGAGTA TGCAAGCTTC AAATCAACAT
    AATTTTGTGC AACAAGCCAT ACAAACATCT GAGCAGTTGA TATATGCCCA CCCTGCTCTA
    GCGACTCCTG ATCAGACTGT GTACACTGAA AAAAATGCAC CATCAGCAGA ACAGCCAATA
    TTTGTACAGC AGGTTGTCTC GTCTGAACAA CCAGCATACA CACAACAGGT TATATCTGCT
    GAGCAGAAAG GCACACAGCC AGCTATTCCT GCAGAGCAGC CATTGTACAC CCAACAAACT
    GTTCCAGCTG AGCAGCCAGT GTATACTCAG CAAGTTATTC AATCGGAACA GCCAGCTCAC
    GAACAGCAAG GTATACTTGC TCAGCAGCCA GTGTATGCAC AGCAAGGTAT TCCAACTCAA
    CCAGTGTACA TACAGCAAAA TTTTGCAGCT CAGCACCAAG TGTACACACA ACAAAGCAAT
    TCCGCTGAGA CACCAATATA CACACAAAAG AATATTCAAA CTGACCAAGC AGTTTACACA
    CAGCAAGCTG TCGTTAAAAC TGAACAAACA GGATTCACCC AGCATGCTGA GCAAAAAGTG
    CTTGTACTCC AAGCAGACCA GACGATATAC GCACCGCAAC AGCCAATCCA AGTACAGCAA
    GCCGTTCATC TAAAAGAGCA GCCATATTAT GCACAGCAAG CAGCAGCTCA CATTCAGCAG
    TCTGCATCTG CTGAGCAAAC ACAATATCCA CAGTCTGCTT TATCTGCCGA GCAGTCCGTA
    TACTCACAGC AAACTGTATC TGCTGGGCAG TCTGGATATC CACAGCAAGC TTTGTCTGCC
    AAACAACCGC TATATATCCA GCAAGCAGTG TCTGCTGGGC AGCCAGTATA TCCACATCTG
    TCTGTATCCA CTGAGCAGCC ACAATATACA CAACACATTG TTCCCTCTGA TAAACAAATA
    TATACACAGC AAACTGTACC TGCTGAGCAG AGAGTGTACC CTCAGCATAT CGTATCAACT
    GAACAACCAA TGTATTCACA ACAAGTTCTA TCAGCTGAGC GTCCAGCATA TACACATCAA
    GTTGCAGCAC ATCCTGAACG CCTGGTATGC ATCCAACAAG TCCTTCAGCC CACAGAACAC
    CCTGCATATG CTTTACAGTC TATGCCATCC TCAGACAGAC CTGTATATCA ACAAGAATCT
    GCTTTGCTTT CTGGCCATAT GAACACCCAG CCAACTATTC CAGCTCATAC TCAAATTCTG
    TACACACAAG AGGCATTTAT GCCAACAGAA CAGCAGCCAT ATAACCTACA AACTGACAGC
    CCAATATATA TCCAACCAGT TGCTGGTGCT TCTCATCAGG TACCTGAACA ATCGGCCCCT
    ATTGAATCAG TATATAAACA GTCAGCTTCA CAGATGTCAT ATCCTCAGCA TGCAGTATCT
    TCATCTGATA CACAAATGTA TGCAAGCAAG ACGGTGGCTG CAAGTGCTCA GCTTGTGTAT
    GCTGTTCCAC CAGCAGAACA GGTGTACATT CAGCAGACAG GTTCTCTTTC TGAGCAACAA
    CAGTATGCCA GTCAAGCTGC TGTTCAGTCT GACAAGTCAG TTTATAGCTC TCAAACTATA
    CCCCCAGCAC AACAAGCTAT TTATACACAA GCACATCATG CTGTAATATC TGCAGAAAAG
    CCAGTGTTGC ACTCCCAAAA TGTGGAATCT GTTCCTCAGC AGACCTCTTT AAATGCTTCA
    GATGGTCTGT GGTGTATTCA GAAGTCACTG CCTCCTTCAG AGCAGATAGC TTACACTCTG
    CATCCTGCAC CATCTGGAGT GCCATCAGTT TGTGCTCAGC AGATGGTTCC CCTTGAACAG
    CTGGTAAACC CAAATTTGCC ATCTAATATT ATGCCACCTC AAGAGTTTAG GTCAGAGCAG
    CTTACTGAGA GTCAGTTGTA TGCACAACAA CACATTCAGT CTCCAAGACC ACTTCCATCA
    ATAATGCAGT CACAAGAGCA TGGGGCAAGT CACACACCCC CTCCTTTACA GACTGCAGAC
    AATCAAAGCT ATGCAAATTC CTTTGGTAAT CAACAAGGAG GTCAAGTGTT GCTACAGCCA
    GATCTTTTGA ACAAACCTTT ACCTGTCCAA CACCAAGCCT TAACTCAGTT ACCATTAGGT
    CAGTCTGATG TGCATTCATC AGATCAAATG AGCACTGCAT TGCCTTCACA AGAGCCTTTG
    AACCAGGCCT CACATGTGCA GCCACAGCAT ATACCAGGAA CTGCACCTCA AGCCCAAAGT
    AATTCAGCAT TTCAACAGAT GCAACCTTTC TCAAGTCAAA CCCAGTCAAC ATGCATTGAG
    CAACCAACAT CTCTTACGCA TTTGCAGCAA CATCCCCCTG TTCAGGTAGC TGGGACAGAT
    AATCCCCAGT CATGTTCAGG ATCTATGCTT CAACAGATAA TTCCACCTGG GTATGCTTCG
    CAGCCAGTTT CACAACAGGT GTGGCCATCT TCAACTCCAG TCTTTTCAGA ATCTCTCTTA
    AAGCATCAGG ATGTTTACCA AAGTGCGGTG GCGGCACCAC AGACTCAATA CAAAGAACCA
    GAGTCAGAGC AGAGTTTGCA GTCTGCATGC CAAGCAGGGG CACCTGAACA GCATGTGTTT
    CAGAAACAAG ATTCTCTTCA GAGTAATGCT CATGGTGACT GTGCCAAAGA CAGCCCAGGT
    GCACACGACA ACACAGCTGG AAATGGCAAG CAAGATAAAA TGAAACAACG GCGAGCTTCC
    TGCCCCCGGC CTGAAAAAAT AGCCAGATTT ATCCTTACTG TAATGCAGGT ATCAACCTCT
    GGAGATAATA TGGTTGAGTG CCAGCTGGAA ACTCATAACA ACAAGATGGT GACTTTTAAA
    TTTGATGCAG ATGGTGATGC TCCGGAAGAT ATTGCCCATT ATATGGTGGA AGATGACTTT
    GTGCTGGAAG TTGAGAAAGA GAAGTTTGTC GAGGAGCTTA GGAATATAGT TACACAGGCC
    CAGGAGATAC TGCGCAGCAT ACCCGCTGAG GAGAGAACTG AATTGATACA GTCAGAGTCT
    AACAGTCAGG CTGGATCCTC AGATCATGTA CAGATGGGAG TCCCAACATT CCAGCAAACG
    GGGGGTGAAA CGGTTCCACA GTCCTCCCCA GTTGGGCGCT GGAGGTTTTT CATCAATCAG
    ACCATAAGGA ACCGCGAATC TCAGACCTCC TCTGTTTCAC AGTCTGCTGT GATCAGGGTT
    CCTCAATTAG CCACATTAGA TAATGAACAA GCAAGTCTCC CTAAGGATTC TTCAGCAGAC
    AGCTGTTCTC TTAAATCTGA GCATACAGTG CCGACTGTTC TTCTTCAGCC AACAGATGGT
    CATGCTTTCC CTACTGAGAC TGAACCTTTG ACTCTCCCTA CTTATGTGAC AGTTGGAAGT
    GAAATGTTAT CATCTCCATT GGACTGTAAT GCCTCAACTT TAGGTCAGAT TCAGCTAGAA
    CCTAGAAACT GTGGTAGTAA TATACTTGTG CTAGCAGAGA GCCAGCAGCC AGAAATACCT
    TCATCTTTTG ATCAGGACGT CCAAGGAGCA AATGGCCTGG TTAACACAAC TGAGACCAGT
    ACTGGACACT TACCGGCACA AAGCACTACA GGCTTGTTAC TTGGCTTAAC TATGTCAGAT
    GCTCTTCATC CAGTTGATAC AACTGTGTTG CCCCAGACTC CCTTGTCAGA AGTTTCCTTA
    GCGCACTCTG CATCAGTGCA AGAGTCTGAC ACTGAAGGAC CCCCTAAAAT AGACTATGTG
    GATAATCACA TCAAAACGTT GGATGAGAAA TTAAGAACTT TGTTATATCA GGACAGCTCT
    GCCAGCTCTT ATGCTGACAG CCAAAAGGAA ACACAGAGTA TAGAATCTCC CTTATCTTCA
    TCTGCTGAGG ATACTTTGTC GTGTCCTGTC CATGAAGCCC TCGATGTGAA TACTGCCGGT
    GTGCAGGCCA CTCCAGAGGA GGCAGACGCT AATGACCCTC CAGCGCAAAG AGATCCTGGA
    GAAGTCATCA CAGTACCTGG TACACTAACA CCATCAGAGA GTTCAGAAGA TGCCTGGGTT
    CCTGGAAGTT CCTCCCACGC TTGTTCCAAG CGCTCTATGG GTACTGGAGC CACGCATCTG
    CAATCAGGAG GTGGATATTT TGGCCTAAGC TTTACTTGTC CTAGTCTCAA AAATCCCATT
    AGCAAGAAAC CGTGGGCTCG CAAATTAAAA AGCTGGGCAT GCAGATTGCG GCATTCCAAC
    AGCTTATTCA AGAGGTCAAG AGTCCGGCAA GTTGAAGAGG AGGGAGTGAG ACTGGACATA
    TCTGATGCCA CACAAAGAGT TGTTGAAGCA TTTGCTGAAT GTGCCTTGTC AGAAGGGTCG
    CCATCAAAAA ACGGCACCTT CAAGCGTGGC CGTTTCCAGG TTGTTTCCGT TCCACAGCAA
    GAGCAGCCCA CTGCAAATGA ATCAGCTGGT GCTCCAGATA CCAAATACCC AGGTAACTCT
    CTTTTACAAG AAACTGGATC CGCAAATACA AAAGCAGAAG ACACTCCGCA GTCTGCCAGC
    ACAGCATGTG AGACTGATGC CTCCTCCCTC ACACCTGATA GAGAACTGGA TGAAACCTCA
    GCCAATGGCA GCAGTGTTCA GTCAAGTTCA GCCTTGTGGA TGAAAGATAT CAAGAAGCCG
    AGCAGCTGTA ATAAACAGCT AAGCAGTGAT TCTGAACAAT CTACTACTCC GGACAGGCCC
    CTGGAATTTA AAGGGAGAGA AAAAGATGCG GGGCAGCAAC CCCAGGGAGA GAAAATGTAT
    AACCAGAAAC AGAATTCCTT CTTTCACTCT CCATCTTCCC CCATGAGCAG TGATGACGAG
    TCCGAGTTAG AAGATGAAGA TTTGAAAGTG GAACTTCAGA AGCTCCGAGA AAAGCACATT
    CAAGAGGTGG TGACCCTTCA AACCCAGCAA AACCACGAGC TCCAGGAGCT GTATGTACGC
    TTGAAGTCTT TAAGGGAAAG CAAAGCACAG TCTTCAGATA CCTCTTCCCA GCCATTATCC
    CCCCGTCGCC CAAGGTCTCT AAAGAGTAAA CTGCGCAGCA GGCCCCAGTC CCTCACTCAC
    ATGGACAATG GGATCGGGCT TTCAGACCAC CAGTGCAGCG AGAGCAATTC CGATGCTTGC
    CAGCAGTCCT TGTCTGAGAA GAAGAGCATG TTCACGGATG ACTTTCATAA GCTGGTGGAT
    GACTGGGCAA AGGAAAATGC TGGCAACCAA GTTTTGAAAC CCAGTCTGAA TCAGATCAAG
    CAGAATCAAA GTCGCCTAGA GTCTGATAAC TGGGGTCGGA CCTATGAGAG TATGACAGCC
    ACATCAGGCT ATCCACCTGC ATGGATCCCT CAGATTCATG GCTCTTTGCC AGCTGCTGTC
    TCTCAGTCTC TCGTGCCCAC CAACTTTACA GCTGGAGCGA TGCCAGCATA CCAAGTGCCA
    CCAGCCTGCC AATTCACTTC AGTGAGCAGT AGTGGTTATC CTGTACCATG GTCTGCGCAA
    GCACCAGTAG TCCCTCCCCA GCATCTCACA GCATACCAGC CTGGCGTCGG AGTTCAAACT
    TTTGCAACCT CAACAGCTCA GAAGGCAACT ACAATCCCAA CATCTCCAAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031746231.1
    CDS165..8738
    Translation

    Target ORF information:

    RefSeq Version XM_031890371.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis WNK lysine deficient protein kinase 3 (wnk3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031890371.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCTACGG ATTCCGGAGA GCCAGCAAGT ACGGATGAAT CTGACAAAGC AGCTGGCTTT 
    TCATTACAAA ATAATCTGTG CTCCCAGGAA GGTTCCTTAA CCAAACAGGC AAGCATGGAG
    GAAAGTGACA AACCTTGCTC ATCTGCAGGG ACCAGTGAAA AGAAACGTTT CTTTAGAAAA
    AGCGTGGACA TTACAGAGGA TGACAGGATG TTGGAGCAGT CTCTAAAAGA TGAGAAAAGC
    ACTGATCCTC TGAGCCCACA GCTGATGGAG GCACATTCAA CAAAGGCTGC TGGTGCAGCA
    CAAGAAGGGA AAAATGATGC CAAAGCTAAT TCAGAGGCCT GTAAAGACTC TCCTAGAGAG
    AAAACTGAAA GGGAGATGGA GGAAGAGGCT GAAATGAAAG CCGTGGCTAC CTCTCCAAGT
    GGCAGATTTC TAAAGTTTGA CATTGAATTG GGCCGAGGGG CCTTTAAGAC AGTCTTCAAA
    GGACTTGACA CTGAAACTTG GGTAGAAGTT GCCTGGTGTG AGCTGCAGGA CAGGAAGTTA
    ACCAAAGCAG AGCAGCAACG ATTTAAAGAA GAGGCCGAGA TGTTAAAGGG CCTTCAGCAC
    CCAAATATTG TGCGGTTTTA TGATTCCTGG GAGTCTTCGC TGAAGGGAAA AAAATGTATA
    GTTTTGGTCA CAGAACTTAT GACATCTGGC ACTTTGAAAA CTTATTTGAA AAGATTTAAG
    GTAATGAAGC CAAAGGTGTT ACGGAGCTGG TGCCGGCAGA TCTTGAAGGG ACTTCAGTTC
    CTGCACACCA GGACACCTCC TATAATCCAT CGTGATCTGA AATGCGACAA CATTTTCATC
    ACTGGTCCCA CAGGCTCAGT GAAGATTGGG GACCTTGGTC TGGCCACCCT GATGCGTACA
    TCATTTGCAA AGAGTGTAAT TGGAACACCA GAATTCATGG CCCCAGAAAT GTATGAAGAG
    CATTACGACG AGTCAGTGGA TGTCTATGCA TTTGGCATGT GCATGCTGGA AATGGCTACA
    TCAGAATACC CATACTCAGA ATGCCAGAAT GCAGCTCAGA TCTACCGCAA AGTAACCAGC
    GGAATCAAAC CTGCCAGCTT CAATAAGGTG TCTGATCCTG AGGTAAAGGA AATAATTGAA
    AGCTGCATCC GTCAGAACAA GGCTGAAAGG TTATCGATAA AAGAACTTCT AAATCATGCT
    TTCTTTGCTG AAGACACTGG CTTGCGAGTT GAACTTGCTG AAGAAGATCA TTGTACAGAT
    TCATCTCTGG CCTTGAGACT TTGGGTGGAG GATCCAAAAA AGCTAAAAGG AAAACATAAA
    GATAATGAAG CTATTGAGTT CAGTTTTAAC TTAGACACGG ACAACCCTGA TGAAGTGGCA
    TGTGAGATGG TGAAATCTGG ATTTTTTCAT GAAAGTGACA GTAAGGCTGT GTCCAAATCT
    ATACGAGACA GAGTGTGTCT TATCAAGAAA ACAAGAGAGC GGAGGATACT GACGGGGTAT
    TCTGAGGACC GGCGTGATTC GCAAAGCAGA ATTGGAGGGG CAACACCTGC TCAGGTAGCA
    GTTCCCATTC CTGCAGTGTA CCAACAACCA GCTGGACACG AGAGTGAAGA AACGGAGGTA
    GACCAGCATG TACGCCAGCA ACTACTGCAA CAGCAGCAGC TTCAGCAGTG CTCTACAGTC
    ACAGTGTGCT CAACAGTCCC TGCTGACAGT CATTCGGAAG CAGGGGCAGG ATCTGTCATG
    CATTCGGATA CTACAAGCCA GCACAGTGCA GTATATGCTA TGAGCCATGA ACAGCTGAGT
    TGCCAGCAAG TGTCTGGGGT GCCTCAGGTA GAAAGTAGTC ATGTGGGGCA AGTCTACCAG
    TCCCAGCAAG TAGTGGGGCA GTATCAACAA ACACAGCAGG TTACTCAGCC GCAAATGCTT
    CCTGCACAAT GCCTTGCTCA GCCTGTCTCA TCTTATCTTC AGTCAGCAGC GGAAGGGCAG
    CTTGGCACAC AGTGCCTGGA TGCGAAGATG ACGTTTGCTG CAAATGGGAA TACTCTTTCG
    CAGTGTAACG CTTTAGATCC TTCAGTGATG TCAGTATTGC AGTCATCCTT TTCTTCGTTG
    TCCCCTAAAA TAATACCTGG ACAAATGACA TCACAGCAAG CAGGGGCTGT TATGCAGCTG
    GATGCTACTA TGACTGGAAT ACAAATTGTT ACACCTGCTG CAACGGACAC CCAAGTTCCT
    GCCCTTCTTC CTGTCTGCCA CCCAGCTTTA CTGGGTCAAT ACAGCAGCAA CATTCAGAAT
    GTGCTGCATC CATCTCAGAC TGTCCATGGG GCACCAGTTG GTCCTCAGCA GACGCAGCAC
    CCTGGAGCAT CTGTGCAGCA ACCAATAATT CCTACTTCAG ATACTTTAAA TTATTCAGCA
    ACGCTTCAGC CCAATATCCA GATACCATCT CACATAGAGC ATCTGCAAAT CTCTCAGCAG
    TCTAGTTACC ATCCTTCACT GCCTCCACAG CAACTTATAT TGCAGCCTGC ACTCACAGAA
    CAACCCCGAA ATCAAAACTT TTCTGATGCT GTTAAAGTTG AAGCTGTCCC ACAACAACTA
    CACATGGAAC CCCATTCAGA ACAGTTAGTA TTCAATGCAA AGACCTCGTC TCTGCATGGT
    GAGATGACAT CATTTGAACC ATCTGGGCAT TTACAGCAGT CTCTCACCTT GAATTCCTTG
    AATGTGCGTG AACAGTCTTC ATATGCCCAG CCTCCATTAC CTCACCTCGG AGTGCAACAA
    CTTTCCAGAG ATGTGGAACA AGTAGCAAAA ACAGTCATGA ACAATCCTCC AGAACAACCA
    CTATACATTC AGATTCCCCC ACTGGACACA TATGGACAAC AGTCTGTTAT TGGTTCTGAT
    CAGATGATAT ATACTCAGAA AGTTATTCCA TCTGATCAGC CTTTATTTTT AGAAAGTACT
    TCCCACTTGA CTAATCAGTC AGTGTATACC CAACAGAGTA TGCAAGCTTC AAATCAACAT
    AATTTTGTGC AACAAGCCAT ACAAACATCT GAGCAGTTGA TATATGCCCA CCCTGCTCTA
    GCGACTCCTG ATCAGACTGT GTACACTGAA AAAAATGCAC CATCAGCAGA ACAGCCAATA
    TTTGTACAGC AGGTTGTCTC GTCTGAACAA CCAGCATACA CACAACAGGT TATATCTGCT
    GAGCAGAAAG GCACACAGCC AGCTATTCCT GCAGAGCAGC CATTGTACAC CCAACAAACT
    GTTCCAGCTG AGCAGCCAGT GTATACTCAG CAAGTTATTC AATCGGAACA GCCAGCTCAC
    GAACAGCAAG GTATACTTGC TCAGCAGCCA GTGTATGCAC AGCAAGGTAT TCCAACTCAA
    CCAGTGTACA TACAGCAAAA TTTTGCAGCT CAGCACCAAG TGTACACACA ACAAAGCAAT
    TCCGCTGAGA CACCAATATA CACACAAAAG AATATTCAAA CTGACCAAGC AGTTTACACA
    CAGCAAGCTG TCGTTAAAAC TGAACAAACA GGATTCACCC AGCATGCTGA GCAAAAAGTG
    CTTGTACTCC AAGCAGACCA GACGATATAC GCACCGCAAC AGCCAATCCA AGTACAGCAA
    GCCGTTCATC TAAAAGAGCA GCCATATTAT GCACAGCAAG CAGCAGCTCA CATTCAGCAG
    TCTGCATCTG CTGAGCAAAC ACAATATCCA CAGTCTGCTT TATCTGCCGA GCAGTCCGTA
    TACTCACAGC AAACTGTATC TGCTGGGCAG TCTGGATATC CACAGCAAGC TTTGTCTGCC
    AAACAACCGC TATATATCCA GCAAGCAGTG TCTGCTGGGC AGCCAGTATA TCCACATCTG
    TCTGTATCCA CTGAGCAGCC ACAATATACA CAACACATTG TTCCCTCTGA TAAACAAATA
    TATACACAGC AAACTGTACC TGCTGAGCAG AGAGTGTACC CTCAGCATAT CGTATCAACT
    GAACAACCAA TGTATTCACA ACAAGTTCTA TCAGCTGAGC GTCCAGCATA TACACATCAA
    GTTGCAGCAC ATCCTGAACG CCTGGTATGC ATCCAACAAG TCCTTCAGCC CACAGAACAC
    CCTGCATATG CTTTACAGTC TATGCCATCC TCAGACAGAC CTGTATATCA ACAAGAATCT
    GCTTTGCTTT CTGGCCATAT GAACACCCAG CCAACTATTC CAGCTCATAC TCAAATTCTG
    TACACACAAG AGGCATTTAT GCCAACAGAA CAGCAGCCAT ATAACCTACA AACTGACAGC
    CCAATATATA TCCAACCAGT TGCTGGTGCT TCTCATCAGG TACCTGAACA ATCGGCCCCT
    ATTGAATCAG TATATAAACA GTCAGCTTCA CAGATGTCAT ATCCTCAGCA TGCAGTATCT
    TCATCTGATA CACAAATGTA TGCAAGCAAG ACGGTGGCTG CAAGTGCTCA GCTTGTGTAT
    GCTGTTCCAC CAGCAGAACA GGTGTACATT CAGCAGACAG GTTCTCTTTC TGAGCAACAA
    CAGTATGCCA GTCAAGCTGC TGTTCAGTCT GACAAGTCAG TTTATAGCTC TCAAACTATA
    CCCCCAGCAC AACAAGCTAT TTATACACAA GCACATCATG CTGTAATATC TGCAGAAAAG
    CCAGTGTTGC ACTCCCAAAA TGTGGAATCT GTTCCTCAGC AGACCTCTTT AAATGCTTCA
    GATGGTCTGT GGTGTATTCA GAAGTCACTG CCTCCTTCAG AGCAGATAGC TTACACTCTG
    CATCCTGCAC CATCTGGAGT GCCATCAGTT TGTGCTCAGC AGATGGTTCC CCTTGAACAG
    CTGGTAAACC CAAATTTGCC ATCTAATATT ATGCCACCTC AAGAGTTTAG GTCAGAGCAG
    CTTACTGAGA GTCAGTTGTA TGCACAACAA CACATTCAGT CTCCAAGACC ACTTCCATCA
    ATAATGCAGT CACAAGAGCA TGGGGCAAGT CACACACCCC CTCCTTTACA GACTGCAGAC
    AATCAAAGCT ATGCAAATTC CTTTGGTAAT CAACAAGGAG GTCAAGTGTT GCTACAGCCA
    GATCTTTTGA ACAAACCTTT ACCTGTCCAA CACCAAGCCT TAACTCAGTT ACCATTAGGT
    CAGTCTGATG TGCATTCATC AGATCAAATG AGCACTGCAT TGCCTTCACA AGAGCCTTTG
    AACCAGGCCT CACATGTGCA GCCACAGCAT ATACCAGGAA CTGCACCTCA AGCCCAAAGT
    AATTCAGCAT TTCAACAGAT GCAACCTTTC TCAAGTCAAA CCCAGTCAAC ATGCATTGAG
    CAACCAACAT CTCTTACGCA TTTGCAGCAA CATCCCCCTG TTCAGGTAGC TGGGACAGAT
    AATCCCCAGT CATGTTCAGG ATCTATGCTT CAACAGATAA TTCCACCTGG GTATGCTTCG
    CAGCCAGTTT CACAACAGGT GTGGCCATCT TCAACTCCAG TCTTTTCAGA ATCTCTCTTA
    AAGCATCAGG ATGTTTACCA AAGTGCGGTG GCGGCACCAC AGACTCAATA CAAAGAACCA
    GAGTCAGAGC AGAGTTTGCA GTCTGCATGC CAAGCAGGGG CACCTGAACA GCATGTGTTT
    CAGAAACAAG ATTCTCTTCA GAGTAATGCT CATGGTGACT GTGCCAAAGA CAGCCCAGGT
    GCACACGACA ACACAGCTGG AAATGGCAAG CAAGATAAAA TGAAACAACG GCGAGCTTCC
    TGCCCCCGGC CTGAAAAAAT AGCCAGATTT ATCCTTACTG TAATGCAGGT ATCAACCTCT
    GGAGATAATA TGGTTGAGTG CCAGCTGGAA ACTCATAACA ACAAGATGGT GACTTTTAAA
    TTTGATGCAG ATGGTGATGC TCCGGAAGAT ATTGCCCATT ATATGGTGGA AGATGACTTT
    GTGCTGGAAG TTGAGAAAGA GAAGTTTGTC GAGGAGCTTA GGAATATAGT TACACAGGCC
    CAGGAGATAC TGCGCAGCAT ACCCGCTGAG GAGAGAACTG AATTGATACA GTCAGAGTCT
    AACAGTCAGG CTGGATCCTC AGATCATGTA CAGATGGGAG TCCCAACATT CCAGCAAACG
    GGGGGTGAAA CGGTTCCACA GTCCTCCCCA GTTGGGCGCT GGAGGTTTTT CATCAATCAG
    ACCATAAGGA ACCGCGAATC TCAGACCTCC TCTGTTTCAC AGTCTGCTGT GATCAGGGTT
    CCTCAATTAG CCACATTAGA TAATGAACAA GCAAGTCTCC CTAAGGATTC TTCAGCAGAC
    AGCTGTTCTC TTAAATCTGA GCATACAGTG CCGACTGTTC TTCTTCAGCC AACAGATGGT
    CATGCTTTCC CTACTGAGAC TGAACCTTTG ACTCTCCCTA CTTATGTGAC AGTTGGAAGT
    GAAATGTTAT CATCTCCATT GGACTGTAAT GCCTCAACTT TAGGTCAGAT TCAGCTAGAA
    CCTAGAAACT GTGGTAGTAA TATACTTGTG CTAGCAGAGA GCCAGCAGCC AGAAATACCT
    TCATCTTTTG ATCAGGACGT CCAAGGAGCA AATGGCCTGG TTAACACAAC TGAGACCAGT
    ACTGGACACT TACCGGCACA AAGCACTACA GGCTTGTTAC TTGGCTTAAC TATGTCAGAT
    GCTCTTCATC CAGTTGATAC AACTGTGTTG CCCCAGACTC CCTTGTCAGA AGTTTCCTTA
    GCGCACTCTG CATCAGTGCA AGAGTCTGAC ACTGAAGGAC CCCCTAAAAT AGACTATGTG
    GATAATCACA TCAAAACGTT GGATGAGAAA TTAAGAACTT TGTTATATCA GGACAGCTCT
    GCCAGCTCTT ATGCTGACAG CCAAAAGGAA ACACAGAGTA TAGAATCTCC CTTATCTTCA
    TCTGCTGAGG ATACTTTGTC GTGTCCTGTC CATGAAGCCC TCGATGTGAA TACTGCCGGT
    GTGCAGGCCA CTCCAGAGGA GGCAGACGCT AATGACCCTC CAGCGCAAAG AGATCCTGGA
    GAAGTCATCA CAGTACCTGG TACACTAACA CCATCAGAGA GTTCAGAAGA TGCCTGGGTT
    CCTGGAAGTT CCTCCCACGC TTGTTCCAAG CGCTCTATGG GTACTGGAGC CACGCATCTG
    CAATCAGGAG GTGGATATTT TGGCCTAAGC TTTACTTGTC CTAGTCTCAA AAATCCCATT
    AGCAAGAAAC CGTGGGCTCG CAAATTAAAA AGCTGGGCAT GCAGATTGCG GCATTCCAAC
    AGCTTATTCA AGAGGTCAAG AGTCCGGCAA GTTGAAGAGG AGGGAGTGAG ACTGGACATA
    TCTGATGCCA CACAAAGAGT TGTTGAAGCA TTTGCTGAAT GTGCCTTGTC AGAAGGGTCG
    CCATCAAAAA ACGGCACCTT CAAGCGTGGC CGTTTCCAGG TTGTTTCCGT TCCACAGCAA
    GAGCAGCCCA CTGCAAATGA ATCAGCTGGT GCTCCAGATA CCAAATACCC AGGTAACTCT
    CTTTTACAAG AAACTGGATC CGCAAATACA AAAGCAGAAG ACACTCCGCA GTCTGCCAGC
    ACAGCATGTG AGACTGATGC CTCCTCCCTC ACACCTGATA GAGAACTGGA TGAAACCTCA
    GCCAATGGCA GCAGTGTTCA GTCAAGTTCA GCCTTGTGGA TGAAAGATAT CAAGAAGCCG
    AGCAGCTGTA ATAAACAGCT AAGCAGTGAT TCTGAACAAT CTACTACTCC GGACAGGCCC
    CTGGAATTTA AAGGGAGAGA AAAAGATGCG GGGCAGCAAC CCCAGGGAGA GAAAATGTAT
    AACCAGAAAC AGAATTCCTT CTTTCACTCT CCATCTTCCC CCATGAGCAG TGATGACGAG
    TCCGAGTTAG AAGATGAAGA TTTGAAAGTG GAACTTCAGA AGCTCCGAGA AAAGCACATT
    CAAGAGGTGG TGACCCTTCA AACCCAGCAA AACCACGAGC TCCAGGAGCT GTATGTACGC
    TTGAAGTCTT TAAGGGAAAG CAAAGCACAG TCTTCAGATA CCTCTTCCCA GCCATTATCC
    CCCCGTCGCC CAAGGTCTCT AAAGAGTAAA CTGCGCAGCA GGCCCCAGTC CCTCACTCAC
    ATGGACAATG GGATCGGGCT TTCAGACCAC CAGTGCAGCG AGAGCAATTC CGATGCTTGC
    CAGCAGTCCT TGTCTGAGAA GAAGAGCATG TTCACGGATG ACTTTCATAA GCTGGTGGAT
    GACTGGGCAA AGGAAAATGC TGGCAACCAA GTTTTGAAAC CCAGTCTGAA TCAGATCAAG
    CAGAATCAAA GTCGCCTAGA GTCTGATAAC TGGGGTCGGA CCTATGAGAG TATGACAGCC
    ACATCAGGCT ATCCACCTGC ATGGATCCCT CAGATTCATG GCTCTTTGCC AGCTGCTGTC
    TCTCAGTCTC TCGTGCCCAC CAACTTTACA GCTGGAGCGA TGCCAGCATA CCAAGTGCCA
    CCAGCCTGCC AATTCACTTC AGTGAGCAGT AGTGGTTATC CTGTACCATG GTCTGCGCAA
    GCACCAGTAG TCCCTCCCCA GCATCTCACA GCATACCAGC CTGGCGTCGG AGTTCAAACT
    TTTGCAACCT CAACAGCTCA GAAGGCAACT ACAATCCCAA CATCTCCAAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa63622
    Clone ID Related Accession (Same CDS sequence) XM_031890370.1 , XM_031890369.1 , XM_031890371.1 , XM_031890372.1
    Accession Version XM_031890372.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8574bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product serine/threonine-protein kinase WNK3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCTACGG ATTCCGGAGA GCCAGCAAGT ACGGATGAAT CTGACAAAGC AGCTGGCTTT 
    TCATTACAAA ATAATCTGTG CTCCCAGGAA GGTTCCTTAA CCAAACAGGC AAGCATGGAG
    GAAAGTGACA AACCTTGCTC ATCTGCAGGG ACCAGTGAAA AGAAACGTTT CTTTAGAAAA
    AGCGTGGACA TTACAGAGGA TGACAGGATG TTGGAGCAGT CTCTAAAAGA TGAGAAAAGC
    ACTGATCCTC TGAGCCCACA GCTGATGGAG GCACATTCAA CAAAGGCTGC TGGTGCAGCA
    CAAGAAGGGA AAAATGATGC CAAAGCTAAT TCAGAGGCCT GTAAAGACTC TCCTAGAGAG
    AAAACTGAAA GGGAGATGGA GGAAGAGGCT GAAATGAAAG CCGTGGCTAC CTCTCCAAGT
    GGCAGATTTC TAAAGTTTGA CATTGAATTG GGCCGAGGGG CCTTTAAGAC AGTCTTCAAA
    GGACTTGACA CTGAAACTTG GGTAGAAGTT GCCTGGTGTG AGCTGCAGGA CAGGAAGTTA
    ACCAAAGCAG AGCAGCAACG ATTTAAAGAA GAGGCCGAGA TGTTAAAGGG CCTTCAGCAC
    CCAAATATTG TGCGGTTTTA TGATTCCTGG GAGTCTTCGC TGAAGGGAAA AAAATGTATA
    GTTTTGGTCA CAGAACTTAT GACATCTGGC ACTTTGAAAA CTTATTTGAA AAGATTTAAG
    GTAATGAAGC CAAAGGTGTT ACGGAGCTGG TGCCGGCAGA TCTTGAAGGG ACTTCAGTTC
    CTGCACACCA GGACACCTCC TATAATCCAT CGTGATCTGA AATGCGACAA CATTTTCATC
    ACTGGTCCCA CAGGCTCAGT GAAGATTGGG GACCTTGGTC TGGCCACCCT GATGCGTACA
    TCATTTGCAA AGAGTGTAAT TGGAACACCA GAATTCATGG CCCCAGAAAT GTATGAAGAG
    CATTACGACG AGTCAGTGGA TGTCTATGCA TTTGGCATGT GCATGCTGGA AATGGCTACA
    TCAGAATACC CATACTCAGA ATGCCAGAAT GCAGCTCAGA TCTACCGCAA AGTAACCAGC
    GGAATCAAAC CTGCCAGCTT CAATAAGGTG TCTGATCCTG AGGTAAAGGA AATAATTGAA
    AGCTGCATCC GTCAGAACAA GGCTGAAAGG TTATCGATAA AAGAACTTCT AAATCATGCT
    TTCTTTGCTG AAGACACTGG CTTGCGAGTT GAACTTGCTG AAGAAGATCA TTGTACAGAT
    TCATCTCTGG CCTTGAGACT TTGGGTGGAG GATCCAAAAA AGCTAAAAGG AAAACATAAA
    GATAATGAAG CTATTGAGTT CAGTTTTAAC TTAGACACGG ACAACCCTGA TGAAGTGGCA
    TGTGAGATGG TGAAATCTGG ATTTTTTCAT GAAAGTGACA GTAAGGCTGT GTCCAAATCT
    ATACGAGACA GAGTGTGTCT TATCAAGAAA ACAAGAGAGC GGAGGATACT GACGGGGTAT
    TCTGAGGACC GGCGTGATTC GCAAAGCAGA ATTGGAGGGG CAACACCTGC TCAGGTAGCA
    GTTCCCATTC CTGCAGTGTA CCAACAACCA GCTGGACACG AGAGTGAAGA AACGGAGGTA
    GACCAGCATG TACGCCAGCA ACTACTGCAA CAGCAGCAGC TTCAGCAGTG CTCTACAGTC
    ACAGTGTGCT CAACAGTCCC TGCTGACAGT CATTCGGAAG CAGGGGCAGG ATCTGTCATG
    CATTCGGATA CTACAAGCCA GCACAGTGCA GTATATGCTA TGAGCCATGA ACAGCTGAGT
    TGCCAGCAAG TGTCTGGGGT GCCTCAGGTA GAAAGTAGTC ATGTGGGGCA AGTCTACCAG
    TCCCAGCAAG TAGTGGGGCA GTATCAACAA ACACAGCAGG TTACTCAGCC GCAAATGCTT
    CCTGCACAAT GCCTTGCTCA GCCTGTCTCA TCTTATCTTC AGTCAGCAGC GGAAGGGCAG
    CTTGGCACAC AGTGCCTGGA TGCGAAGATG ACGTTTGCTG CAAATGGGAA TACTCTTTCG
    CAGTGTAACG CTTTAGATCC TTCAGTGATG TCAGTATTGC AGTCATCCTT TTCTTCGTTG
    TCCCCTAAAA TAATACCTGG ACAAATGACA TCACAGCAAG CAGGGGCTGT TATGCAGCTG
    GATGCTACTA TGACTGGAAT ACAAATTGTT ACACCTGCTG CAACGGACAC CCAAGTTCCT
    GCCCTTCTTC CTGTCTGCCA CCCAGCTTTA CTGGGTCAAT ACAGCAGCAA CATTCAGAAT
    GTGCTGCATC CATCTCAGAC TGTCCATGGG GCACCAGTTG GTCCTCAGCA GACGCAGCAC
    CCTGGAGCAT CTGTGCAGCA ACCAATAATT CCTACTTCAG ATACTTTAAA TTATTCAGCA
    ACGCTTCAGC CCAATATCCA GATACCATCT CACATAGAGC ATCTGCAAAT CTCTCAGCAG
    TCTAGTTACC ATCCTTCACT GCCTCCACAG CAACTTATAT TGCAGCCTGC ACTCACAGAA
    CAACCCCGAA ATCAAAACTT TTCTGATGCT GTTAAAGTTG AAGCTGTCCC ACAACAACTA
    CACATGGAAC CCCATTCAGA ACAGTTAGTA TTCAATGCAA AGACCTCGTC TCTGCATGGT
    GAGATGACAT CATTTGAACC ATCTGGGCAT TTACAGCAGT CTCTCACCTT GAATTCCTTG
    AATGTGCGTG AACAGTCTTC ATATGCCCAG CCTCCATTAC CTCACCTCGG AGTGCAACAA
    CTTTCCAGAG ATGTGGAACA AGTAGCAAAA ACAGTCATGA ACAATCCTCC AGAACAACCA
    CTATACATTC AGATTCCCCC ACTGGACACA TATGGACAAC AGTCTGTTAT TGGTTCTGAT
    CAGATGATAT ATACTCAGAA AGTTATTCCA TCTGATCAGC CTTTATTTTT AGAAAGTACT
    TCCCACTTGA CTAATCAGTC AGTGTATACC CAACAGAGTA TGCAAGCTTC AAATCAACAT
    AATTTTGTGC AACAAGCCAT ACAAACATCT GAGCAGTTGA TATATGCCCA CCCTGCTCTA
    GCGACTCCTG ATCAGACTGT GTACACTGAA AAAAATGCAC CATCAGCAGA ACAGCCAATA
    TTTGTACAGC AGGTTGTCTC GTCTGAACAA CCAGCATACA CACAACAGGT TATATCTGCT
    GAGCAGAAAG GCACACAGCC AGCTATTCCT GCAGAGCAGC CATTGTACAC CCAACAAACT
    GTTCCAGCTG AGCAGCCAGT GTATACTCAG CAAGTTATTC AATCGGAACA GCCAGCTCAC
    GAACAGCAAG GTATACTTGC TCAGCAGCCA GTGTATGCAC AGCAAGGTAT TCCAACTCAA
    CCAGTGTACA TACAGCAAAA TTTTGCAGCT CAGCACCAAG TGTACACACA ACAAAGCAAT
    TCCGCTGAGA CACCAATATA CACACAAAAG AATATTCAAA CTGACCAAGC AGTTTACACA
    CAGCAAGCTG TCGTTAAAAC TGAACAAACA GGATTCACCC AGCATGCTGA GCAAAAAGTG
    CTTGTACTCC AAGCAGACCA GACGATATAC GCACCGCAAC AGCCAATCCA AGTACAGCAA
    GCCGTTCATC TAAAAGAGCA GCCATATTAT GCACAGCAAG CAGCAGCTCA CATTCAGCAG
    TCTGCATCTG CTGAGCAAAC ACAATATCCA CAGTCTGCTT TATCTGCCGA GCAGTCCGTA
    TACTCACAGC AAACTGTATC TGCTGGGCAG TCTGGATATC CACAGCAAGC TTTGTCTGCC
    AAACAACCGC TATATATCCA GCAAGCAGTG TCTGCTGGGC AGCCAGTATA TCCACATCTG
    TCTGTATCCA CTGAGCAGCC ACAATATACA CAACACATTG TTCCCTCTGA TAAACAAATA
    TATACACAGC AAACTGTACC TGCTGAGCAG AGAGTGTACC CTCAGCATAT CGTATCAACT
    GAACAACCAA TGTATTCACA ACAAGTTCTA TCAGCTGAGC GTCCAGCATA TACACATCAA
    GTTGCAGCAC ATCCTGAACG CCTGGTATGC ATCCAACAAG TCCTTCAGCC CACAGAACAC
    CCTGCATATG CTTTACAGTC TATGCCATCC TCAGACAGAC CTGTATATCA ACAAGAATCT
    GCTTTGCTTT CTGGCCATAT GAACACCCAG CCAACTATTC CAGCTCATAC TCAAATTCTG
    TACACACAAG AGGCATTTAT GCCAACAGAA CAGCAGCCAT ATAACCTACA AACTGACAGC
    CCAATATATA TCCAACCAGT TGCTGGTGCT TCTCATCAGG TACCTGAACA ATCGGCCCCT
    ATTGAATCAG TATATAAACA GTCAGCTTCA CAGATGTCAT ATCCTCAGCA TGCAGTATCT
    TCATCTGATA CACAAATGTA TGCAAGCAAG ACGGTGGCTG CAAGTGCTCA GCTTGTGTAT
    GCTGTTCCAC CAGCAGAACA GGTGTACATT CAGCAGACAG GTTCTCTTTC TGAGCAACAA
    CAGTATGCCA GTCAAGCTGC TGTTCAGTCT GACAAGTCAG TTTATAGCTC TCAAACTATA
    CCCCCAGCAC AACAAGCTAT TTATACACAA GCACATCATG CTGTAATATC TGCAGAAAAG
    CCAGTGTTGC ACTCCCAAAA TGTGGAATCT GTTCCTCAGC AGACCTCTTT AAATGCTTCA
    GATGGTCTGT GGTGTATTCA GAAGTCACTG CCTCCTTCAG AGCAGATAGC TTACACTCTG
    CATCCTGCAC CATCTGGAGT GCCATCAGTT TGTGCTCAGC AGATGGTTCC CCTTGAACAG
    CTGGTAAACC CAAATTTGCC ATCTAATATT ATGCCACCTC AAGAGTTTAG GTCAGAGCAG
    CTTACTGAGA GTCAGTTGTA TGCACAACAA CACATTCAGT CTCCAAGACC ACTTCCATCA
    ATAATGCAGT CACAAGAGCA TGGGGCAAGT CACACACCCC CTCCTTTACA GACTGCAGAC
    AATCAAAGCT ATGCAAATTC CTTTGGTAAT CAACAAGGAG GTCAAGTGTT GCTACAGCCA
    GATCTTTTGA ACAAACCTTT ACCTGTCCAA CACCAAGCCT TAACTCAGTT ACCATTAGGT
    CAGTCTGATG TGCATTCATC AGATCAAATG AGCACTGCAT TGCCTTCACA AGAGCCTTTG
    AACCAGGCCT CACATGTGCA GCCACAGCAT ATACCAGGAA CTGCACCTCA AGCCCAAAGT
    AATTCAGCAT TTCAACAGAT GCAACCTTTC TCAAGTCAAA CCCAGTCAAC ATGCATTGAG
    CAACCAACAT CTCTTACGCA TTTGCAGCAA CATCCCCCTG TTCAGGTAGC TGGGACAGAT
    AATCCCCAGT CATGTTCAGG ATCTATGCTT CAACAGATAA TTCCACCTGG GTATGCTTCG
    CAGCCAGTTT CACAACAGGT GTGGCCATCT TCAACTCCAG TCTTTTCAGA ATCTCTCTTA
    AAGCATCAGG ATGTTTACCA AAGTGCGGTG GCGGCACCAC AGACTCAATA CAAAGAACCA
    GAGTCAGAGC AGAGTTTGCA GTCTGCATGC CAAGCAGGGG CACCTGAACA GCATGTGTTT
    CAGAAACAAG ATTCTCTTCA GAGTAATGCT CATGGTGACT GTGCCAAAGA CAGCCCAGGT
    GCACACGACA ACACAGCTGG AAATGGCAAG CAAGATAAAA TGAAACAACG GCGAGCTTCC
    TGCCCCCGGC CTGAAAAAAT AGCCAGATTT ATCCTTACTG TAATGCAGGT ATCAACCTCT
    GGAGATAATA TGGTTGAGTG CCAGCTGGAA ACTCATAACA ACAAGATGGT GACTTTTAAA
    TTTGATGCAG ATGGTGATGC TCCGGAAGAT ATTGCCCATT ATATGGTGGA AGATGACTTT
    GTGCTGGAAG TTGAGAAAGA GAAGTTTGTC GAGGAGCTTA GGAATATAGT TACACAGGCC
    CAGGAGATAC TGCGCAGCAT ACCCGCTGAG GAGAGAACTG AATTGATACA GTCAGAGTCT
    AACAGTCAGG CTGGATCCTC AGATCATGTA CAGATGGGAG TCCCAACATT CCAGCAAACG
    GGGGGTGAAA CGGTTCCACA GTCCTCCCCA GTTGGGCGCT GGAGGTTTTT CATCAATCAG
    ACCATAAGGA ACCGCGAATC TCAGACCTCC TCTGTTTCAC AGTCTGCTGT GATCAGGGTT
    CCTCAATTAG CCACATTAGA TAATGAACAA GCAAGTCTCC CTAAGGATTC TTCAGCAGAC
    AGCTGTTCTC TTAAATCTGA GCATACAGTG CCGACTGTTC TTCTTCAGCC AACAGATGGT
    CATGCTTTCC CTACTGAGAC TGAACCTTTG ACTCTCCCTA CTTATGTGAC AGTTGGAAGT
    GAAATGTTAT CATCTCCATT GGACTGTAAT GCCTCAACTT TAGGTCAGAT TCAGCTAGAA
    CCTAGAAACT GTGGTAGTAA TATACTTGTG CTAGCAGAGA GCCAGCAGCC AGAAATACCT
    TCATCTTTTG ATCAGGACGT CCAAGGAGCA AATGGCCTGG TTAACACAAC TGAGACCAGT
    ACTGGACACT TACCGGCACA AAGCACTACA GGCTTGTTAC TTGGCTTAAC TATGTCAGAT
    GCTCTTCATC CAGTTGATAC AACTGTGTTG CCCCAGACTC CCTTGTCAGA AGTTTCCTTA
    GCGCACTCTG CATCAGTGCA AGAGTCTGAC ACTGAAGGAC CCCCTAAAAT AGACTATGTG
    GATAATCACA TCAAAACGTT GGATGAGAAA TTAAGAACTT TGTTATATCA GGACAGCTCT
    GCCAGCTCTT ATGCTGACAG CCAAAAGGAA ACACAGAGTA TAGAATCTCC CTTATCTTCA
    TCTGCTGAGG ATACTTTGTC GTGTCCTGTC CATGAAGCCC TCGATGTGAA TACTGCCGGT
    GTGCAGGCCA CTCCAGAGGA GGCAGACGCT AATGACCCTC CAGCGCAAAG AGATCCTGGA
    GAAGTCATCA CAGTACCTGG TACACTAACA CCATCAGAGA GTTCAGAAGA TGCCTGGGTT
    CCTGGAAGTT CCTCCCACGC TTGTTCCAAG CGCTCTATGG GTACTGGAGC CACGCATCTG
    CAATCAGGAG GTGGATATTT TGGCCTAAGC TTTACTTGTC CTAGTCTCAA AAATCCCATT
    AGCAAGAAAC CGTGGGCTCG CAAATTAAAA AGCTGGGCAT GCAGATTGCG GCATTCCAAC
    AGCTTATTCA AGAGGTCAAG AGTCCGGCAA GTTGAAGAGG AGGGAGTGAG ACTGGACATA
    TCTGATGCCA CACAAAGAGT TGTTGAAGCA TTTGCTGAAT GTGCCTTGTC AGAAGGGTCG
    CCATCAAAAA ACGGCACCTT CAAGCGTGGC CGTTTCCAGG TTGTTTCCGT TCCACAGCAA
    GAGCAGCCCA CTGCAAATGA ATCAGCTGGT GCTCCAGATA CCAAATACCC AGGTAACTCT
    CTTTTACAAG AAACTGGATC CGCAAATACA AAAGCAGAAG ACACTCCGCA GTCTGCCAGC
    ACAGCATGTG AGACTGATGC CTCCTCCCTC ACACCTGATA GAGAACTGGA TGAAACCTCA
    GCCAATGGCA GCAGTGTTCA GTCAAGTTCA GCCTTGTGGA TGAAAGATAT CAAGAAGCCG
    AGCAGCTGTA ATAAACAGCT AAGCAGTGAT TCTGAACAAT CTACTACTCC GGACAGGCCC
    CTGGAATTTA AAGGGAGAGA AAAAGATGCG GGGCAGCAAC CCCAGGGAGA GAAAATGTAT
    AACCAGAAAC AGAATTCCTT CTTTCACTCT CCATCTTCCC CCATGAGCAG TGATGACGAG
    TCCGAGTTAG AAGATGAAGA TTTGAAAGTG GAACTTCAGA AGCTCCGAGA AAAGCACATT
    CAAGAGGTGG TGACCCTTCA AACCCAGCAA AACCACGAGC TCCAGGAGCT GTATGTACGC
    TTGAAGTCTT TAAGGGAAAG CAAAGCACAG TCTTCAGATA CCTCTTCCCA GCCATTATCC
    CCCCGTCGCC CAAGGTCTCT AAAGAGTAAA CTGCGCAGCA GGCCCCAGTC CCTCACTCAC
    ATGGACAATG GGATCGGGCT TTCAGACCAC CAGTGCAGCG AGAGCAATTC CGATGCTTGC
    CAGCAGTCCT TGTCTGAGAA GAAGAGCATG TTCACGGATG ACTTTCATAA GCTGGTGGAT
    GACTGGGCAA AGGAAAATGC TGGCAACCAA GTTTTGAAAC CCAGTCTGAA TCAGATCAAG
    CAGAATCAAA GTCGCCTAGA GTCTGATAAC TGGGGTCGGA CCTATGAGAG TATGACAGCC
    ACATCAGGCT ATCCACCTGC ATGGATCCCT CAGATTCATG GCTCTTTGCC AGCTGCTGTC
    TCTCAGTCTC TCGTGCCCAC CAACTTTACA GCTGGAGCGA TGCCAGCATA CCAAGTGCCA
    CCAGCCTGCC AATTCACTTC AGTGAGCAGT AGTGGTTATC CTGTACCATG GTCTGCGCAA
    GCACCAGTAG TCCCTCCCCA GCATCTCACA GCATACCAGC CTGGCGTCGG AGTTCAAACT
    TTTGCAACCT CAACAGCTCA GAAGGCAACT ACAATCCCAA CATCTCCAAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031746232.1
    CDS244..8817
    Translation

    Target ORF information:

    RefSeq Version XM_031890372.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis WNK lysine deficient protein kinase 3 (wnk3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031890372.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCTACGG ATTCCGGAGA GCCAGCAAGT ACGGATGAAT CTGACAAAGC AGCTGGCTTT 
    TCATTACAAA ATAATCTGTG CTCCCAGGAA GGTTCCTTAA CCAAACAGGC AAGCATGGAG
    GAAAGTGACA AACCTTGCTC ATCTGCAGGG ACCAGTGAAA AGAAACGTTT CTTTAGAAAA
    AGCGTGGACA TTACAGAGGA TGACAGGATG TTGGAGCAGT CTCTAAAAGA TGAGAAAAGC
    ACTGATCCTC TGAGCCCACA GCTGATGGAG GCACATTCAA CAAAGGCTGC TGGTGCAGCA
    CAAGAAGGGA AAAATGATGC CAAAGCTAAT TCAGAGGCCT GTAAAGACTC TCCTAGAGAG
    AAAACTGAAA GGGAGATGGA GGAAGAGGCT GAAATGAAAG CCGTGGCTAC CTCTCCAAGT
    GGCAGATTTC TAAAGTTTGA CATTGAATTG GGCCGAGGGG CCTTTAAGAC AGTCTTCAAA
    GGACTTGACA CTGAAACTTG GGTAGAAGTT GCCTGGTGTG AGCTGCAGGA CAGGAAGTTA
    ACCAAAGCAG AGCAGCAACG ATTTAAAGAA GAGGCCGAGA TGTTAAAGGG CCTTCAGCAC
    CCAAATATTG TGCGGTTTTA TGATTCCTGG GAGTCTTCGC TGAAGGGAAA AAAATGTATA
    GTTTTGGTCA CAGAACTTAT GACATCTGGC ACTTTGAAAA CTTATTTGAA AAGATTTAAG
    GTAATGAAGC CAAAGGTGTT ACGGAGCTGG TGCCGGCAGA TCTTGAAGGG ACTTCAGTTC
    CTGCACACCA GGACACCTCC TATAATCCAT CGTGATCTGA AATGCGACAA CATTTTCATC
    ACTGGTCCCA CAGGCTCAGT GAAGATTGGG GACCTTGGTC TGGCCACCCT GATGCGTACA
    TCATTTGCAA AGAGTGTAAT TGGAACACCA GAATTCATGG CCCCAGAAAT GTATGAAGAG
    CATTACGACG AGTCAGTGGA TGTCTATGCA TTTGGCATGT GCATGCTGGA AATGGCTACA
    TCAGAATACC CATACTCAGA ATGCCAGAAT GCAGCTCAGA TCTACCGCAA AGTAACCAGC
    GGAATCAAAC CTGCCAGCTT CAATAAGGTG TCTGATCCTG AGGTAAAGGA AATAATTGAA
    AGCTGCATCC GTCAGAACAA GGCTGAAAGG TTATCGATAA AAGAACTTCT AAATCATGCT
    TTCTTTGCTG AAGACACTGG CTTGCGAGTT GAACTTGCTG AAGAAGATCA TTGTACAGAT
    TCATCTCTGG CCTTGAGACT TTGGGTGGAG GATCCAAAAA AGCTAAAAGG AAAACATAAA
    GATAATGAAG CTATTGAGTT CAGTTTTAAC TTAGACACGG ACAACCCTGA TGAAGTGGCA
    TGTGAGATGG TGAAATCTGG ATTTTTTCAT GAAAGTGACA GTAAGGCTGT GTCCAAATCT
    ATACGAGACA GAGTGTGTCT TATCAAGAAA ACAAGAGAGC GGAGGATACT GACGGGGTAT
    TCTGAGGACC GGCGTGATTC GCAAAGCAGA ATTGGAGGGG CAACACCTGC TCAGGTAGCA
    GTTCCCATTC CTGCAGTGTA CCAACAACCA GCTGGACACG AGAGTGAAGA AACGGAGGTA
    GACCAGCATG TACGCCAGCA ACTACTGCAA CAGCAGCAGC TTCAGCAGTG CTCTACAGTC
    ACAGTGTGCT CAACAGTCCC TGCTGACAGT CATTCGGAAG CAGGGGCAGG ATCTGTCATG
    CATTCGGATA CTACAAGCCA GCACAGTGCA GTATATGCTA TGAGCCATGA ACAGCTGAGT
    TGCCAGCAAG TGTCTGGGGT GCCTCAGGTA GAAAGTAGTC ATGTGGGGCA AGTCTACCAG
    TCCCAGCAAG TAGTGGGGCA GTATCAACAA ACACAGCAGG TTACTCAGCC GCAAATGCTT
    CCTGCACAAT GCCTTGCTCA GCCTGTCTCA TCTTATCTTC AGTCAGCAGC GGAAGGGCAG
    CTTGGCACAC AGTGCCTGGA TGCGAAGATG ACGTTTGCTG CAAATGGGAA TACTCTTTCG
    CAGTGTAACG CTTTAGATCC TTCAGTGATG TCAGTATTGC AGTCATCCTT TTCTTCGTTG
    TCCCCTAAAA TAATACCTGG ACAAATGACA TCACAGCAAG CAGGGGCTGT TATGCAGCTG
    GATGCTACTA TGACTGGAAT ACAAATTGTT ACACCTGCTG CAACGGACAC CCAAGTTCCT
    GCCCTTCTTC CTGTCTGCCA CCCAGCTTTA CTGGGTCAAT ACAGCAGCAA CATTCAGAAT
    GTGCTGCATC CATCTCAGAC TGTCCATGGG GCACCAGTTG GTCCTCAGCA GACGCAGCAC
    CCTGGAGCAT CTGTGCAGCA ACCAATAATT CCTACTTCAG ATACTTTAAA TTATTCAGCA
    ACGCTTCAGC CCAATATCCA GATACCATCT CACATAGAGC ATCTGCAAAT CTCTCAGCAG
    TCTAGTTACC ATCCTTCACT GCCTCCACAG CAACTTATAT TGCAGCCTGC ACTCACAGAA
    CAACCCCGAA ATCAAAACTT TTCTGATGCT GTTAAAGTTG AAGCTGTCCC ACAACAACTA
    CACATGGAAC CCCATTCAGA ACAGTTAGTA TTCAATGCAA AGACCTCGTC TCTGCATGGT
    GAGATGACAT CATTTGAACC ATCTGGGCAT TTACAGCAGT CTCTCACCTT GAATTCCTTG
    AATGTGCGTG AACAGTCTTC ATATGCCCAG CCTCCATTAC CTCACCTCGG AGTGCAACAA
    CTTTCCAGAG ATGTGGAACA AGTAGCAAAA ACAGTCATGA ACAATCCTCC AGAACAACCA
    CTATACATTC AGATTCCCCC ACTGGACACA TATGGACAAC AGTCTGTTAT TGGTTCTGAT
    CAGATGATAT ATACTCAGAA AGTTATTCCA TCTGATCAGC CTTTATTTTT AGAAAGTACT
    TCCCACTTGA CTAATCAGTC AGTGTATACC CAACAGAGTA TGCAAGCTTC AAATCAACAT
    AATTTTGTGC AACAAGCCAT ACAAACATCT GAGCAGTTGA TATATGCCCA CCCTGCTCTA
    GCGACTCCTG ATCAGACTGT GTACACTGAA AAAAATGCAC CATCAGCAGA ACAGCCAATA
    TTTGTACAGC AGGTTGTCTC GTCTGAACAA CCAGCATACA CACAACAGGT TATATCTGCT
    GAGCAGAAAG GCACACAGCC AGCTATTCCT GCAGAGCAGC CATTGTACAC CCAACAAACT
    GTTCCAGCTG AGCAGCCAGT GTATACTCAG CAAGTTATTC AATCGGAACA GCCAGCTCAC
    GAACAGCAAG GTATACTTGC TCAGCAGCCA GTGTATGCAC AGCAAGGTAT TCCAACTCAA
    CCAGTGTACA TACAGCAAAA TTTTGCAGCT CAGCACCAAG TGTACACACA ACAAAGCAAT
    TCCGCTGAGA CACCAATATA CACACAAAAG AATATTCAAA CTGACCAAGC AGTTTACACA
    CAGCAAGCTG TCGTTAAAAC TGAACAAACA GGATTCACCC AGCATGCTGA GCAAAAAGTG
    CTTGTACTCC AAGCAGACCA GACGATATAC GCACCGCAAC AGCCAATCCA AGTACAGCAA
    GCCGTTCATC TAAAAGAGCA GCCATATTAT GCACAGCAAG CAGCAGCTCA CATTCAGCAG
    TCTGCATCTG CTGAGCAAAC ACAATATCCA CAGTCTGCTT TATCTGCCGA GCAGTCCGTA
    TACTCACAGC AAACTGTATC TGCTGGGCAG TCTGGATATC CACAGCAAGC TTTGTCTGCC
    AAACAACCGC TATATATCCA GCAAGCAGTG TCTGCTGGGC AGCCAGTATA TCCACATCTG
    TCTGTATCCA CTGAGCAGCC ACAATATACA CAACACATTG TTCCCTCTGA TAAACAAATA
    TATACACAGC AAACTGTACC TGCTGAGCAG AGAGTGTACC CTCAGCATAT CGTATCAACT
    GAACAACCAA TGTATTCACA ACAAGTTCTA TCAGCTGAGC GTCCAGCATA TACACATCAA
    GTTGCAGCAC ATCCTGAACG CCTGGTATGC ATCCAACAAG TCCTTCAGCC CACAGAACAC
    CCTGCATATG CTTTACAGTC TATGCCATCC TCAGACAGAC CTGTATATCA ACAAGAATCT
    GCTTTGCTTT CTGGCCATAT GAACACCCAG CCAACTATTC CAGCTCATAC TCAAATTCTG
    TACACACAAG AGGCATTTAT GCCAACAGAA CAGCAGCCAT ATAACCTACA AACTGACAGC
    CCAATATATA TCCAACCAGT TGCTGGTGCT TCTCATCAGG TACCTGAACA ATCGGCCCCT
    ATTGAATCAG TATATAAACA GTCAGCTTCA CAGATGTCAT ATCCTCAGCA TGCAGTATCT
    TCATCTGATA CACAAATGTA TGCAAGCAAG ACGGTGGCTG CAAGTGCTCA GCTTGTGTAT
    GCTGTTCCAC CAGCAGAACA GGTGTACATT CAGCAGACAG GTTCTCTTTC TGAGCAACAA
    CAGTATGCCA GTCAAGCTGC TGTTCAGTCT GACAAGTCAG TTTATAGCTC TCAAACTATA
    CCCCCAGCAC AACAAGCTAT TTATACACAA GCACATCATG CTGTAATATC TGCAGAAAAG
    CCAGTGTTGC ACTCCCAAAA TGTGGAATCT GTTCCTCAGC AGACCTCTTT AAATGCTTCA
    GATGGTCTGT GGTGTATTCA GAAGTCACTG CCTCCTTCAG AGCAGATAGC TTACACTCTG
    CATCCTGCAC CATCTGGAGT GCCATCAGTT TGTGCTCAGC AGATGGTTCC CCTTGAACAG
    CTGGTAAACC CAAATTTGCC ATCTAATATT ATGCCACCTC AAGAGTTTAG GTCAGAGCAG
    CTTACTGAGA GTCAGTTGTA TGCACAACAA CACATTCAGT CTCCAAGACC ACTTCCATCA
    ATAATGCAGT CACAAGAGCA TGGGGCAAGT CACACACCCC CTCCTTTACA GACTGCAGAC
    AATCAAAGCT ATGCAAATTC CTTTGGTAAT CAACAAGGAG GTCAAGTGTT GCTACAGCCA
    GATCTTTTGA ACAAACCTTT ACCTGTCCAA CACCAAGCCT TAACTCAGTT ACCATTAGGT
    CAGTCTGATG TGCATTCATC AGATCAAATG AGCACTGCAT TGCCTTCACA AGAGCCTTTG
    AACCAGGCCT CACATGTGCA GCCACAGCAT ATACCAGGAA CTGCACCTCA AGCCCAAAGT
    AATTCAGCAT TTCAACAGAT GCAACCTTTC TCAAGTCAAA CCCAGTCAAC ATGCATTGAG
    CAACCAACAT CTCTTACGCA TTTGCAGCAA CATCCCCCTG TTCAGGTAGC TGGGACAGAT
    AATCCCCAGT CATGTTCAGG ATCTATGCTT CAACAGATAA TTCCACCTGG GTATGCTTCG
    CAGCCAGTTT CACAACAGGT GTGGCCATCT TCAACTCCAG TCTTTTCAGA ATCTCTCTTA
    AAGCATCAGG ATGTTTACCA AAGTGCGGTG GCGGCACCAC AGACTCAATA CAAAGAACCA
    GAGTCAGAGC AGAGTTTGCA GTCTGCATGC CAAGCAGGGG CACCTGAACA GCATGTGTTT
    CAGAAACAAG ATTCTCTTCA GAGTAATGCT CATGGTGACT GTGCCAAAGA CAGCCCAGGT
    GCACACGACA ACACAGCTGG AAATGGCAAG CAAGATAAAA TGAAACAACG GCGAGCTTCC
    TGCCCCCGGC CTGAAAAAAT AGCCAGATTT ATCCTTACTG TAATGCAGGT ATCAACCTCT
    GGAGATAATA TGGTTGAGTG CCAGCTGGAA ACTCATAACA ACAAGATGGT GACTTTTAAA
    TTTGATGCAG ATGGTGATGC TCCGGAAGAT ATTGCCCATT ATATGGTGGA AGATGACTTT
    GTGCTGGAAG TTGAGAAAGA GAAGTTTGTC GAGGAGCTTA GGAATATAGT TACACAGGCC
    CAGGAGATAC TGCGCAGCAT ACCCGCTGAG GAGAGAACTG AATTGATACA GTCAGAGTCT
    AACAGTCAGG CTGGATCCTC AGATCATGTA CAGATGGGAG TCCCAACATT CCAGCAAACG
    GGGGGTGAAA CGGTTCCACA GTCCTCCCCA GTTGGGCGCT GGAGGTTTTT CATCAATCAG
    ACCATAAGGA ACCGCGAATC TCAGACCTCC TCTGTTTCAC AGTCTGCTGT GATCAGGGTT
    CCTCAATTAG CCACATTAGA TAATGAACAA GCAAGTCTCC CTAAGGATTC TTCAGCAGAC
    AGCTGTTCTC TTAAATCTGA GCATACAGTG CCGACTGTTC TTCTTCAGCC AACAGATGGT
    CATGCTTTCC CTACTGAGAC TGAACCTTTG ACTCTCCCTA CTTATGTGAC AGTTGGAAGT
    GAAATGTTAT CATCTCCATT GGACTGTAAT GCCTCAACTT TAGGTCAGAT TCAGCTAGAA
    CCTAGAAACT GTGGTAGTAA TATACTTGTG CTAGCAGAGA GCCAGCAGCC AGAAATACCT
    TCATCTTTTG ATCAGGACGT CCAAGGAGCA AATGGCCTGG TTAACACAAC TGAGACCAGT
    ACTGGACACT TACCGGCACA AAGCACTACA GGCTTGTTAC TTGGCTTAAC TATGTCAGAT
    GCTCTTCATC CAGTTGATAC AACTGTGTTG CCCCAGACTC CCTTGTCAGA AGTTTCCTTA
    GCGCACTCTG CATCAGTGCA AGAGTCTGAC ACTGAAGGAC CCCCTAAAAT AGACTATGTG
    GATAATCACA TCAAAACGTT GGATGAGAAA TTAAGAACTT TGTTATATCA GGACAGCTCT
    GCCAGCTCTT ATGCTGACAG CCAAAAGGAA ACACAGAGTA TAGAATCTCC CTTATCTTCA
    TCTGCTGAGG ATACTTTGTC GTGTCCTGTC CATGAAGCCC TCGATGTGAA TACTGCCGGT
    GTGCAGGCCA CTCCAGAGGA GGCAGACGCT AATGACCCTC CAGCGCAAAG AGATCCTGGA
    GAAGTCATCA CAGTACCTGG TACACTAACA CCATCAGAGA GTTCAGAAGA TGCCTGGGTT
    CCTGGAAGTT CCTCCCACGC TTGTTCCAAG CGCTCTATGG GTACTGGAGC CACGCATCTG
    CAATCAGGAG GTGGATATTT TGGCCTAAGC TTTACTTGTC CTAGTCTCAA AAATCCCATT
    AGCAAGAAAC CGTGGGCTCG CAAATTAAAA AGCTGGGCAT GCAGATTGCG GCATTCCAAC
    AGCTTATTCA AGAGGTCAAG AGTCCGGCAA GTTGAAGAGG AGGGAGTGAG ACTGGACATA
    TCTGATGCCA CACAAAGAGT TGTTGAAGCA TTTGCTGAAT GTGCCTTGTC AGAAGGGTCG
    CCATCAAAAA ACGGCACCTT CAAGCGTGGC CGTTTCCAGG TTGTTTCCGT TCCACAGCAA
    GAGCAGCCCA CTGCAAATGA ATCAGCTGGT GCTCCAGATA CCAAATACCC AGGTAACTCT
    CTTTTACAAG AAACTGGATC CGCAAATACA AAAGCAGAAG ACACTCCGCA GTCTGCCAGC
    ACAGCATGTG AGACTGATGC CTCCTCCCTC ACACCTGATA GAGAACTGGA TGAAACCTCA
    GCCAATGGCA GCAGTGTTCA GTCAAGTTCA GCCTTGTGGA TGAAAGATAT CAAGAAGCCG
    AGCAGCTGTA ATAAACAGCT AAGCAGTGAT TCTGAACAAT CTACTACTCC GGACAGGCCC
    CTGGAATTTA AAGGGAGAGA AAAAGATGCG GGGCAGCAAC CCCAGGGAGA GAAAATGTAT
    AACCAGAAAC AGAATTCCTT CTTTCACTCT CCATCTTCCC CCATGAGCAG TGATGACGAG
    TCCGAGTTAG AAGATGAAGA TTTGAAAGTG GAACTTCAGA AGCTCCGAGA AAAGCACATT
    CAAGAGGTGG TGACCCTTCA AACCCAGCAA AACCACGAGC TCCAGGAGCT GTATGTACGC
    TTGAAGTCTT TAAGGGAAAG CAAAGCACAG TCTTCAGATA CCTCTTCCCA GCCATTATCC
    CCCCGTCGCC CAAGGTCTCT AAAGAGTAAA CTGCGCAGCA GGCCCCAGTC CCTCACTCAC
    ATGGACAATG GGATCGGGCT TTCAGACCAC CAGTGCAGCG AGAGCAATTC CGATGCTTGC
    CAGCAGTCCT TGTCTGAGAA GAAGAGCATG TTCACGGATG ACTTTCATAA GCTGGTGGAT
    GACTGGGCAA AGGAAAATGC TGGCAACCAA GTTTTGAAAC CCAGTCTGAA TCAGATCAAG
    CAGAATCAAA GTCGCCTAGA GTCTGATAAC TGGGGTCGGA CCTATGAGAG TATGACAGCC
    ACATCAGGCT ATCCACCTGC ATGGATCCCT CAGATTCATG GCTCTTTGCC AGCTGCTGTC
    TCTCAGTCTC TCGTGCCCAC CAACTTTACA GCTGGAGCGA TGCCAGCATA CCAAGTGCCA
    CCAGCCTGCC AATTCACTTC AGTGAGCAGT AGTGGTTATC CTGTACCATG GTCTGCGCAA
    GCACCAGTAG TCCCTCCCCA GCATCTCACA GCATACCAGC CTGGCGTCGG AGTTCAAACT
    TTTGCAACCT CAACAGCTCA GAAGGCAACT ACAATCCCAA CATCTCCAAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa63623
    Clone ID Related Accession (Same CDS sequence) XM_031890373.1
    Accession Version XM_031890373.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8571bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product serine/threonine-protein kinase WNK3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCTACGG ATTCCGGAGA GCCAGCAAGT ACGGATGAAT CTGACAAAGC AGCTGGCTTT 
    TCATTACAAA ATAATCTGTG CTCCCAGGAA GGTTCCTTAA CCAAACAGGC AAGCATGGAG
    GAAAGTGACA AACCTTGCTC ATCTGCAGGG ACCAGTGAAA AGAAACGTTT CTTTAGAAAA
    AGCGTGGACA TTACAGAGGA TGACAGGATG TTGGAGCAGT CTCTAAAAGA TGAGAAAAGC
    ACTGATCCTC TGAGCCCACA GCTGATGGAG GCACATTCAA CAAAGGCTGC TGGTGCAGCA
    CAAGAAGGGA AAAATGATGC CAAAGCTAAT TCAGAGGCCT GTAAAGACTC TCCTAGAGAG
    AAAACTGAAA GGGAGATGGA GGAAGAGGCT GAAATGAAAG CCGTGGCTAC CTCTCCAAGT
    GGCAGATTTC TAAAGTTTGA CATTGAATTG GGCCGAGGGG CCTTTAAGAC AGTCTTCAAA
    GGACTTGACA CTGAAACTTG GGTAGAAGTT GCCTGGTGTG AGCTGCAGGA CAGGAAGTTA
    ACCAAAGCAG AGCAGCAACG ATTTAAAGAA GAGGCCGAGA TGTTAAAGGG CCTTCAGCAC
    CCAAATATTG TGCGGTTTTA TGATTCCTGG GAGTCTTCGC TGAAGGGAAA AAAATGTATA
    GTTTTGGTCA CAGAACTTAT GACATCTGGC ACTTTGAAAA CTTATTTGAA AAGATTTAAG
    GTAATGAAGC CAAAGGTGTT ACGGAGCTGG TGCCGGCAGA TCTTGAAGGG ACTTCAGTTC
    CTGCACACCA GGACACCTCC TATAATCCAT CGTGATCTGA AATGCGACAA CATTTTCATC
    ACTGGTCCCA CAGGCTCAGT GAAGATTGGG GACCTTGGTC TGGCCACCCT GATGCGTACA
    TCATTTGCAA AGAGTGTAAT TGGAACACCA GAATTCATGG CCCCAGAAAT GTATGAAGAG
    CATTACGACG AGTCAGTGGA TGTCTATGCA TTTGGCATGT GCATGCTGGA AATGGCTACA
    TCAGAATACC CATACTCAGA ATGCCAGAAT GCAGCTCAGA TCTACCGCAA AGTAACCAGC
    GGAATCAAAC CTGCCAGCTT CAATAAGGTG TCTGATCCTG AGGTAAAGGA AATAATTGAA
    AGCTGCATCC GTCAGAACAA GGCTGAAAGG TTATCGATAA AAGAACTTCT AAATCATGCT
    TTCTTTGCTG AAGACACTGG CTTGCGAGTT GAACTTGCTG AAGAAGATCA TTGTACAGAT
    TCATCTCTGG CCTTGAGACT TTGGGTGGAG GATCCAAAAA AGCTAAAAGG AAAACATAAA
    GATAATGAAG CTATTGAGTT CAGTTTTAAC TTAGACACGG ACAACCCTGA TGAAGTGGCA
    TGTGAGATGG TGAAATCTGG ATTTTTTCAT GAAAGTGACA GTAAGGCTGT GTCCAAATCT
    ATACGAGACA GAGTGTGTCT TATCAAGAAA ACAAGAGAGC GGAGGATACT GACGGGGTAT
    TCTGAGGACC GGCGTGATTC GCAAAGCAGA ATTGGAGGGG CAACACCTGC TCAGGTAGCA
    GTTCCCATTC CTGCAGTGTA CCAACAACCA GCTGGACACG AGAGTGAAGA AACGGAGGTA
    GACCAGCATG TACGCCAGCA ACTACTGCAA CAGCAGCAGC TTCAGCAGTG CTCTACAGTC
    ACAGTGTGCT CAACAGTCCC TGCTGACAGT CATTCGGAAG CAGGGGCAGG ATCTGTCATG
    CATTCGGATA CTACAAGCCA GCACAGTGCA GTATATGCTA TGAGCCATGA ACAGCTGAGT
    TGCCAGCAAG TGTCTGGGGT GCCTCAGGTA GAAAGTAGTC ATGTGGGGCA AGTCTACCAG
    TCCCAGCAAG TAGTGGGGCA GTATCAACAA ACACAGCAGG TTACTCAGCC GCAAATGCTT
    CCTGCACAAT GCCTTGCTCA GCCTGTCTCA TCTTATCTTC AGTCAGCAGC GGAAGGGCAG
    CTTGGCACAC AGTGCCTGGA TGCGAAGATG ACGTTTGCTG CAAATGGGAA TACTCTTTCG
    CAGTGTAACG CTTTAGATCC TTCAGTGATG TCAGTATTGC AGTCATCCTT TTCTTCGTTG
    TCCCCTAAAA TAATACCTGG ACAAATGACA TCACAGCAAG CAGGGGCTGT TATGCAGCTG
    GATGCTACTA TGACTGGAAT ACAAATTGTT ACACCTGCTG CAACGGACAC CCAAGTTCCT
    GCCCTTCTTC CTGTCTGCCA CCCAGCTTTA CTGGGTCAAT ACAGCAGCAA CATTCAGAAT
    GTGCTGCATC CATCTCAGAC TGTCCATGGG GCACCAGTTG GTCCTCAGCA GACGCAGCAC
    CCTGGAGCAT CTGTGCAGCA ACCAATAATT CCTACTTCAG ATACTTTAAA TTATTCAGCA
    ACGCTTCAGC CCAATATCCA GATACCATCT CACATAGAGC ATCTGCAAAT CTCTCAGCAG
    TCTAGTTACC ATCCTTCACT GCCTCCACAG CAACTTATAT TGCAGCCTGC ACTCACAGAA
    CAACCCCGAA ATCAAAACTT TTCTGATGCT GTTAAAGTTG AAGCTGTCCC ACAACAACTA
    CACATGGAAC CCCATTCAGA ACAGTTAGTA TTCAATGCAA AGACCTCGTC TCTGCATGGT
    GAGATGACAT CATTTGAACC ATCTGGGCAT TTACAGCAGT CTCTCACCTT GAATTCCTTG
    AATGTGCGTG AACAGTCTTC ATATGCCCAG CCTCCATTAC CTCACCTCGG AGTGCAACAA
    CTTTCCAGAG ATGTGGAACA AGTAGCAAAA ACAGTCATGA ACAATCCTCC AGAACAACCA
    CTATACATTC AGATTCCCCC ACTGGACACA TATGGACAAC AGTCTGTTAT TGGTTCTGAT
    CAGATGATAT ATACTCAGAA AGTTATTCCA TCTGATCAGC CTTTATTTTT AGAAAGTACT
    TCCCACTTGA CTAATCAGTC AGTGTATACC CAACAGAGTA TGCAAGCTTC AAATCAACAT
    AATTTTGTGC AACAAGCCAT ACAAACATCT GAGCAGTTGA TATATGCCCA CCCTGCTCTA
    GCGACTCCTG ATCAGACTGT GTACACTGAA AAAAATGCAC CATCAGCAGA ACAGCCAATA
    TTTGTACAGC AGGTTGTCTC GTCTGAACAA CCAGCATACA CACAACAGGT TATATCTGCT
    GAGCAGAAAG GCACACAGCC AGCTATTCCT GCAGAGCAGC CATTGTACAC CCAACAAACT
    GTTCCAGCTG AGCAGCCAGT GTATACTCAG CAAGTTATTC AATCGGAACA GCCAGCTCAC
    GAACAGCAAG GTATACTTGC TCAGCAGCCA GTGTATGCAC AGCAAGGTAT TCCAACTCAA
    CCAGTGTACA TACAGCAAAA TTTTGCAGCT CAGCACCAAG TGTACACACA ACAAAGCAAT
    TCCGCTGAGA CACCAATATA CACACAAAAG AATATTCAAA CTGACCAAGC AGTTTACACA
    CAGCAAGCTG TCGTTAAAAC TGAACAAACA GGATTCACCC AGCATGCTGA GCAAAAAGTG
    CTTGTACTCC AAGCAGACCA GACGATATAC GCACCGCAAC AGCCAATCCA AGTACAGCAA
    GCCGTTCATC TAAAAGAGCA GCCATATTAT GCACAGCAAG CAGCAGCTCA CATTCAGCAG
    TCTGCATCTG CTGAGCAAAC ACAATATCCA CAGTCTGCTT TATCTGCCGA GCAGTCCGTA
    TACTCACAGC AAACTGTATC TGCTGGGCAG TCTGGATATC CACAGCAAGC TTTGTCTGCC
    AAACAACCGC TATATATCCA GCAAGCAGTG TCTGCTGGGC AGCCAGTATA TCCACATCTG
    TCTGTATCCA CTGAGCAGCC ACAATATACA CAACACATTG TTCCCTCTGA TAAACAAATA
    TATACACAGC AAACTGTACC TGCTGAGCAG AGAGTGTACC CTCAGCATAT CGTATCAACT
    GAACAACCAA TGTATTCACA ACAAGTTCTA TCAGCTGAGC GTCCAGCATA TACACATCAA
    GTTGCAGCAC ATCCTGAACG CCTGGTATGC ATCCAACAAG TCCTTCAGCC CACAGAACAC
    CCTGCATATG CTTTACAGTC TATGCCATCC TCAGACAGAC CTGTATATCA ACAAGAATCT
    GCTTTGCTTT CTGGCCATAT GAACACCCAG CCAACTATTC CAGCTCATAC TCAAATTCTG
    TACACACAAG AGGCATTTAT GCCAACAGAA CAGCAGCCAT ATAACCTACA AACTGACAGC
    CCAATATATA TCCAACCAGT TGCTGGTGCT TCTCATCAGG TACCTGAACA ATCGGCCCCT
    ATTGAATCAG TATATAAACA GTCAGCTTCA CAGATGTCAT ATCCTCAGCA TGCAGTATCT
    TCATCTGATA CACAAATGTA TGCAAGCAAG ACGGTGGCTG CAAGTGCTCA GCTTGTGTAT
    GCTGTTCCAC CAGCAGAACA GGTGTACATT CAGCAGACAG GTTCTCTTTC TGAGCAACAA
    CAGTATGCCA GTCAAGCTGC TGTTCAGTCT GACAAGTCAG TTTATAGCTC TCAAACTATA
    CCCCCAGCAC AACAAGCTAT TTATACACAA GCACATCATG CTGTAATATC TGCAGAAAAG
    CCAGTGTTGC ACTCCCAAAA TGTGGAATCT GTTCCTCAGC AGACCTCTTT AAATGCTTCA
    GATGGTCTGT GGTGTATTCA GAAGTCACTG CCTCCTTCAG AGCAGATAGC TTACACTCTG
    CATCCTGCAC CATCTGGAGT GCCATCAGTT TGTGCTCAGC AGATGGTTCC CCTTGAACAG
    CTGGTAAACC CAAATTTGCC ATCTAATATT ATGCCACCTC AAGAGTTTAG GTCAGAGCAG
    CTTACTGAGA GTCAGTTGTA TGCACAACAA CACATTCAGT CTCCAAGACC ACTTCCATCA
    ATAATGCAGT CACAAGAGCA TGGGGCAAGT CACACACCCC CTCCTTTACA GACTGCAGAC
    AATCAAAGCT ATGCAAATTC CTTTGGTAAT CAACAAGGAG GTCAAGTGTT GCTACAGCCA
    GATCTTTTGA ACAAACCTTT ACCTGTCCAA CACCAAGCCT TAACTCAGTT ACCATTAGGT
    CAGTCTGATG TGCATTCATC AGATCAAATG AGCACTGCAT TGCCTTCACA AGAGCCTTTG
    AACCAGGCCT CACATGTGCA GCCACAGCAT ATACCAGGAA CTGCACCTCA AGCCCAAAGT
    AATTCAGCAT TTCAACAGAT GCAACCTTTC TCAAGTCAAA CCCAGTCAAC ATGCATTGAG
    CAACCAACAT CTCTTACGCA TTTGCAGCAA CATCCCCCTG TTCAGGTAGC TGGGACAGAT
    AATCCCCAGT CATGTTCAGG ATCTATGCTT CAACAGATAA TTCCACCTGG GTATGCTTCG
    CAGCCAGTTT CACAACAGGT GTGGCCATCT TCAACTCCAG TCTTTTCAGA ATCTCTCTTA
    AAGCATCAGG ATGTTTACCA AAGTGCGGTG GCGGCACCAC AGACTCAATA CAAAGAACCA
    GAGTCAGAGC AGAGTTTGCA GTCTGCATGC CAAGCAGGGG CACCTGAACA GCATGTGTTT
    CAGAAACAAG ATTCTCTTCA GAGTAATGCT CATGGTGACT GTGCCAAAGA CAGCCCAGGT
    GCACACGACA ACACAGCTGG AAATGGCAAG CAAGATAAAA TGAAACAACG GCGAGCTTCC
    TGCCCCCGGC CTGAAAAAAT AGCCAGATTT ATCCTTACTG TAATGCAGGT ATCAACCTCT
    GGAGATAATA TGGTTGAGTG CCAGCTGGAA ACTCATAACA ACAAGATGGT GACTTTTAAA
    TTTGATGCAG ATGGTGATGC TCCGGAAGAT ATTGCCCATT ATATGGTGGA AGATGACTTT
    GTGCTGGAAG TTGAGAAAGA GAAGTTTGTC GAGGAGCTTA GGAATATAGT TACACAGGCC
    CAGGAGATAC TGCGCAGCAT ACCCGCTGAG GAGAGAACTG AATTGATACA GTCAGAGTCT
    AACAGTCAGG CTGGATCCTC AGATCATGTA CAGATGGGAG TCCCAACATT CCAGCAAACG
    GGGGGTGAAA CGGTTCCACA GTCCTCCCCA GTTGGGCGCT GGAGGTTTTT CATCAATCAG
    ACCATAAGGA ACCGCGAATC TCAGACCTCC TCTGTTTCAC AGTCTGCTGT GATCAGGGTT
    CCTCAATTAG CCACATATAA TGAACAAGCA AGTCTCCCTA AGGATTCTTC AGCAGACAGC
    TGTTCTCTTA AATCTGAGCA TACAGTGCCG ACTGTTCTTC TTCAGCCAAC AGATGGTCAT
    GCTTTCCCTA CTGAGACTGA ACCTTTGACT CTCCCTACTT ATGTGACAGT TGGAAGTGAA
    ATGTTATCAT CTCCATTGGA CTGTAATGCC TCAACTTTAG GTCAGATTCA GCTAGAACCT
    AGAAACTGTG GTAGTAATAT ACTTGTGCTA GCAGAGAGCC AGCAGCCAGA AATACCTTCA
    TCTTTTGATC AGGACGTCCA AGGAGCAAAT GGCCTGGTTA ACACAACTGA GACCAGTACT
    GGACACTTAC CGGCACAAAG CACTACAGGC TTGTTACTTG GCTTAACTAT GTCAGATGCT
    CTTCATCCAG TTGATACAAC TGTGTTGCCC CAGACTCCCT TGTCAGAAGT TTCCTTAGCG
    CACTCTGCAT CAGTGCAAGA GTCTGACACT GAAGGACCCC CTAAAATAGA CTATGTGGAT
    AATCACATCA AAACGTTGGA TGAGAAATTA AGAACTTTGT TATATCAGGA CAGCTCTGCC
    AGCTCTTATG CTGACAGCCA AAAGGAAACA CAGAGTATAG AATCTCCCTT ATCTTCATCT
    GCTGAGGATA CTTTGTCGTG TCCTGTCCAT GAAGCCCTCG ATGTGAATAC TGCCGGTGTG
    CAGGCCACTC CAGAGGAGGC AGACGCTAAT GACCCTCCAG CGCAAAGAGA TCCTGGAGAA
    GTCATCACAG TACCTGGTAC ACTAACACCA TCAGAGAGTT CAGAAGATGC CTGGGTTCCT
    GGAAGTTCCT CCCACGCTTG TTCCAAGCGC TCTATGGGTA CTGGAGCCAC GCATCTGCAA
    TCAGGAGGTG GATATTTTGG CCTAAGCTTT ACTTGTCCTA GTCTCAAAAA TCCCATTAGC
    AAGAAACCGT GGGCTCGCAA ATTAAAAAGC TGGGCATGCA GATTGCGGCA TTCCAACAGC
    TTATTCAAGA GGTCAAGAGT CCGGCAAGTT GAAGAGGAGG GAGTGAGACT GGACATATCT
    GATGCCACAC AAAGAGTTGT TGAAGCATTT GCTGAATGTG CCTTGTCAGA AGGGTCGCCA
    TCAAAAAACG GCACCTTCAA GCGTGGCCGT TTCCAGGTTG TTTCCGTTCC ACAGCAAGAG
    CAGCCCACTG CAAATGAATC AGCTGGTGCT CCAGATACCA AATACCCAGG TAACTCTCTT
    TTACAAGAAA CTGGATCCGC AAATACAAAA GCAGAAGACA CTCCGCAGTC TGCCAGCACA
    GCATGTGAGA CTGATGCCTC CTCCCTCACA CCTGATAGAG AACTGGATGA AACCTCAGCC
    AATGGCAGCA GTGTTCAGTC AAGTTCAGCC TTGTGGATGA AAGATATCAA GAAGCCGAGC
    AGCTGTAATA AACAGCTAAG CAGTGATTCT GAACAATCTA CTACTCCGGA CAGGCCCCTG
    GAATTTAAAG GGAGAGAAAA AGATGCGGGG CAGCAACCCC AGGGAGAGAA AATGTATAAC
    CAGAAACAGA ATTCCTTCTT TCACTCTCCA TCTTCCCCCA TGAGCAGTGA TGACGAGTCC
    GAGTTAGAAG ATGAAGATTT GAAAGTGGAA CTTCAGAAGC TCCGAGAAAA GCACATTCAA
    GAGGTGGTGA CCCTTCAAAC CCAGCAAAAC CACGAGCTCC AGGAGCTGTA TGTACGCTTG
    AAGTCTTTAA GGGAAAGCAA AGCACAGTCT TCAGATACCT CTTCCCAGCC ATTATCCCCC
    CGTCGCCCAA GGTCTCTAAA GAGTAAACTG CGCAGCAGGC CCCAGTCCCT CACTCACATG
    GACAATGGGA TCGGGCTTTC AGACCACCAG TGCAGCGAGA GCAATTCCGA TGCTTGCCAG
    CAGTCCTTGT CTGAGAAGAA GAGCATGTTC ACGGATGACT TTCATAAGCT GGTGGATGAC
    TGGGCAAAGG AAAATGCTGG CAACCAAGTT TTGAAACCCA GTCTGAATCA GATCAAGCAG
    AATCAAAGTC GCCTAGAGTC TGATAACTGG GGTCGGACCT ATGAGAGTAT GACAGCCACA
    TCAGGCTATC CACCTGCATG GATCCCTCAG ATTCATGGCT CTTTGCCAGC TGCTGTCTCT
    CAGTCTCTCG TGCCCACCAA CTTTACAGCT GGAGCGATGC CAGCATACCA AGTGCCACCA
    GCCTGCCAAT TCACTTCAGT GAGCAGTAGT GGTTATCCTG TACCATGGTC TGCGCAAGCA
    CCAGTAGTCC CTCCCCAGCA TCTCACAGCA TACCAGCCTG GCGTCGGAGT TCAAACTTTT
    GCAACCTCAA CAGCTCAGAA GGCAACTACA ATCCCAACAT CTCCAAAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031746233.1
    CDS193..8763
    Translation

    Target ORF information:

    RefSeq Version XM_031890373.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis WNK lysine deficient protein kinase 3 (wnk3), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031890373.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCTACGG ATTCCGGAGA GCCAGCAAGT ACGGATGAAT CTGACAAAGC AGCTGGCTTT 
    TCATTACAAA ATAATCTGTG CTCCCAGGAA GGTTCCTTAA CCAAACAGGC AAGCATGGAG
    GAAAGTGACA AACCTTGCTC ATCTGCAGGG ACCAGTGAAA AGAAACGTTT CTTTAGAAAA
    AGCGTGGACA TTACAGAGGA TGACAGGATG TTGGAGCAGT CTCTAAAAGA TGAGAAAAGC
    ACTGATCCTC TGAGCCCACA GCTGATGGAG GCACATTCAA CAAAGGCTGC TGGTGCAGCA
    CAAGAAGGGA AAAATGATGC CAAAGCTAAT TCAGAGGCCT GTAAAGACTC TCCTAGAGAG
    AAAACTGAAA GGGAGATGGA GGAAGAGGCT GAAATGAAAG CCGTGGCTAC CTCTCCAAGT
    GGCAGATTTC TAAAGTTTGA CATTGAATTG GGCCGAGGGG CCTTTAAGAC AGTCTTCAAA
    GGACTTGACA CTGAAACTTG GGTAGAAGTT GCCTGGTGTG AGCTGCAGGA CAGGAAGTTA
    ACCAAAGCAG AGCAGCAACG ATTTAAAGAA GAGGCCGAGA TGTTAAAGGG CCTTCAGCAC
    CCAAATATTG TGCGGTTTTA TGATTCCTGG GAGTCTTCGC TGAAGGGAAA AAAATGTATA
    GTTTTGGTCA CAGAACTTAT GACATCTGGC ACTTTGAAAA CTTATTTGAA AAGATTTAAG
    GTAATGAAGC CAAAGGTGTT ACGGAGCTGG TGCCGGCAGA TCTTGAAGGG ACTTCAGTTC
    CTGCACACCA GGACACCTCC TATAATCCAT CGTGATCTGA AATGCGACAA CATTTTCATC
    ACTGGTCCCA CAGGCTCAGT GAAGATTGGG GACCTTGGTC TGGCCACCCT GATGCGTACA
    TCATTTGCAA AGAGTGTAAT TGGAACACCA GAATTCATGG CCCCAGAAAT GTATGAAGAG
    CATTACGACG AGTCAGTGGA TGTCTATGCA TTTGGCATGT GCATGCTGGA AATGGCTACA
    TCAGAATACC CATACTCAGA ATGCCAGAAT GCAGCTCAGA TCTACCGCAA AGTAACCAGC
    GGAATCAAAC CTGCCAGCTT CAATAAGGTG TCTGATCCTG AGGTAAAGGA AATAATTGAA
    AGCTGCATCC GTCAGAACAA GGCTGAAAGG TTATCGATAA AAGAACTTCT AAATCATGCT
    TTCTTTGCTG AAGACACTGG CTTGCGAGTT GAACTTGCTG AAGAAGATCA TTGTACAGAT
    TCATCTCTGG CCTTGAGACT TTGGGTGGAG GATCCAAAAA AGCTAAAAGG AAAACATAAA
    GATAATGAAG CTATTGAGTT CAGTTTTAAC TTAGACACGG ACAACCCTGA TGAAGTGGCA
    TGTGAGATGG TGAAATCTGG ATTTTTTCAT GAAAGTGACA GTAAGGCTGT GTCCAAATCT
    ATACGAGACA GAGTGTGTCT TATCAAGAAA ACAAGAGAGC GGAGGATACT GACGGGGTAT
    TCTGAGGACC GGCGTGATTC GCAAAGCAGA ATTGGAGGGG CAACACCTGC TCAGGTAGCA
    GTTCCCATTC CTGCAGTGTA CCAACAACCA GCTGGACACG AGAGTGAAGA AACGGAGGTA
    GACCAGCATG TACGCCAGCA ACTACTGCAA CAGCAGCAGC TTCAGCAGTG CTCTACAGTC
    ACAGTGTGCT CAACAGTCCC TGCTGACAGT CATTCGGAAG CAGGGGCAGG ATCTGTCATG
    CATTCGGATA CTACAAGCCA GCACAGTGCA GTATATGCTA TGAGCCATGA ACAGCTGAGT
    TGCCAGCAAG TGTCTGGGGT GCCTCAGGTA GAAAGTAGTC ATGTGGGGCA AGTCTACCAG
    TCCCAGCAAG TAGTGGGGCA GTATCAACAA ACACAGCAGG TTACTCAGCC GCAAATGCTT
    CCTGCACAAT GCCTTGCTCA GCCTGTCTCA TCTTATCTTC AGTCAGCAGC GGAAGGGCAG
    CTTGGCACAC AGTGCCTGGA TGCGAAGATG ACGTTTGCTG CAAATGGGAA TACTCTTTCG
    CAGTGTAACG CTTTAGATCC TTCAGTGATG TCAGTATTGC AGTCATCCTT TTCTTCGTTG
    TCCCCTAAAA TAATACCTGG ACAAATGACA TCACAGCAAG CAGGGGCTGT TATGCAGCTG
    GATGCTACTA TGACTGGAAT ACAAATTGTT ACACCTGCTG CAACGGACAC CCAAGTTCCT
    GCCCTTCTTC CTGTCTGCCA CCCAGCTTTA CTGGGTCAAT ACAGCAGCAA CATTCAGAAT
    GTGCTGCATC CATCTCAGAC TGTCCATGGG GCACCAGTTG GTCCTCAGCA GACGCAGCAC
    CCTGGAGCAT CTGTGCAGCA ACCAATAATT CCTACTTCAG ATACTTTAAA TTATTCAGCA
    ACGCTTCAGC CCAATATCCA GATACCATCT CACATAGAGC ATCTGCAAAT CTCTCAGCAG
    TCTAGTTACC ATCCTTCACT GCCTCCACAG CAACTTATAT TGCAGCCTGC ACTCACAGAA
    CAACCCCGAA ATCAAAACTT TTCTGATGCT GTTAAAGTTG AAGCTGTCCC ACAACAACTA
    CACATGGAAC CCCATTCAGA ACAGTTAGTA TTCAATGCAA AGACCTCGTC TCTGCATGGT
    GAGATGACAT CATTTGAACC ATCTGGGCAT TTACAGCAGT CTCTCACCTT GAATTCCTTG
    AATGTGCGTG AACAGTCTTC ATATGCCCAG CCTCCATTAC CTCACCTCGG AGTGCAACAA
    CTTTCCAGAG ATGTGGAACA AGTAGCAAAA ACAGTCATGA ACAATCCTCC AGAACAACCA
    CTATACATTC AGATTCCCCC ACTGGACACA TATGGACAAC AGTCTGTTAT TGGTTCTGAT
    CAGATGATAT ATACTCAGAA AGTTATTCCA TCTGATCAGC CTTTATTTTT AGAAAGTACT
    TCCCACTTGA CTAATCAGTC AGTGTATACC CAACAGAGTA TGCAAGCTTC AAATCAACAT
    AATTTTGTGC AACAAGCCAT ACAAACATCT GAGCAGTTGA TATATGCCCA CCCTGCTCTA
    GCGACTCCTG ATCAGACTGT GTACACTGAA AAAAATGCAC CATCAGCAGA ACAGCCAATA
    TTTGTACAGC AGGTTGTCTC GTCTGAACAA CCAGCATACA CACAACAGGT TATATCTGCT
    GAGCAGAAAG GCACACAGCC AGCTATTCCT GCAGAGCAGC CATTGTACAC CCAACAAACT
    GTTCCAGCTG AGCAGCCAGT GTATACTCAG CAAGTTATTC AATCGGAACA GCCAGCTCAC
    GAACAGCAAG GTATACTTGC TCAGCAGCCA GTGTATGCAC AGCAAGGTAT TCCAACTCAA
    CCAGTGTACA TACAGCAAAA TTTTGCAGCT CAGCACCAAG TGTACACACA ACAAAGCAAT
    TCCGCTGAGA CACCAATATA CACACAAAAG AATATTCAAA CTGACCAAGC AGTTTACACA
    CAGCAAGCTG TCGTTAAAAC TGAACAAACA GGATTCACCC AGCATGCTGA GCAAAAAGTG
    CTTGTACTCC AAGCAGACCA GACGATATAC GCACCGCAAC AGCCAATCCA AGTACAGCAA
    GCCGTTCATC TAAAAGAGCA GCCATATTAT GCACAGCAAG CAGCAGCTCA CATTCAGCAG
    TCTGCATCTG CTGAGCAAAC ACAATATCCA CAGTCTGCTT TATCTGCCGA GCAGTCCGTA
    TACTCACAGC AAACTGTATC TGCTGGGCAG TCTGGATATC CACAGCAAGC TTTGTCTGCC
    AAACAACCGC TATATATCCA GCAAGCAGTG TCTGCTGGGC AGCCAGTATA TCCACATCTG
    TCTGTATCCA CTGAGCAGCC ACAATATACA CAACACATTG TTCCCTCTGA TAAACAAATA
    TATACACAGC AAACTGTACC TGCTGAGCAG AGAGTGTACC CTCAGCATAT CGTATCAACT
    GAACAACCAA TGTATTCACA ACAAGTTCTA TCAGCTGAGC GTCCAGCATA TACACATCAA
    GTTGCAGCAC ATCCTGAACG CCTGGTATGC ATCCAACAAG TCCTTCAGCC CACAGAACAC
    CCTGCATATG CTTTACAGTC TATGCCATCC TCAGACAGAC CTGTATATCA ACAAGAATCT
    GCTTTGCTTT CTGGCCATAT GAACACCCAG CCAACTATTC CAGCTCATAC TCAAATTCTG
    TACACACAAG AGGCATTTAT GCCAACAGAA CAGCAGCCAT ATAACCTACA AACTGACAGC
    CCAATATATA TCCAACCAGT TGCTGGTGCT TCTCATCAGG TACCTGAACA ATCGGCCCCT
    ATTGAATCAG TATATAAACA GTCAGCTTCA CAGATGTCAT ATCCTCAGCA TGCAGTATCT
    TCATCTGATA CACAAATGTA TGCAAGCAAG ACGGTGGCTG CAAGTGCTCA GCTTGTGTAT
    GCTGTTCCAC CAGCAGAACA GGTGTACATT CAGCAGACAG GTTCTCTTTC TGAGCAACAA
    CAGTATGCCA GTCAAGCTGC TGTTCAGTCT GACAAGTCAG TTTATAGCTC TCAAACTATA
    CCCCCAGCAC AACAAGCTAT TTATACACAA GCACATCATG CTGTAATATC TGCAGAAAAG
    CCAGTGTTGC ACTCCCAAAA TGTGGAATCT GTTCCTCAGC AGACCTCTTT AAATGCTTCA
    GATGGTCTGT GGTGTATTCA GAAGTCACTG CCTCCTTCAG AGCAGATAGC TTACACTCTG
    CATCCTGCAC CATCTGGAGT GCCATCAGTT TGTGCTCAGC AGATGGTTCC CCTTGAACAG
    CTGGTAAACC CAAATTTGCC ATCTAATATT ATGCCACCTC AAGAGTTTAG GTCAGAGCAG
    CTTACTGAGA GTCAGTTGTA TGCACAACAA CACATTCAGT CTCCAAGACC ACTTCCATCA
    ATAATGCAGT CACAAGAGCA TGGGGCAAGT CACACACCCC CTCCTTTACA GACTGCAGAC
    AATCAAAGCT ATGCAAATTC CTTTGGTAAT CAACAAGGAG GTCAAGTGTT GCTACAGCCA
    GATCTTTTGA ACAAACCTTT ACCTGTCCAA CACCAAGCCT TAACTCAGTT ACCATTAGGT
    CAGTCTGATG TGCATTCATC AGATCAAATG AGCACTGCAT TGCCTTCACA AGAGCCTTTG
    AACCAGGCCT CACATGTGCA GCCACAGCAT ATACCAGGAA CTGCACCTCA AGCCCAAAGT
    AATTCAGCAT TTCAACAGAT GCAACCTTTC TCAAGTCAAA CCCAGTCAAC ATGCATTGAG
    CAACCAACAT CTCTTACGCA TTTGCAGCAA CATCCCCCTG TTCAGGTAGC TGGGACAGAT
    AATCCCCAGT CATGTTCAGG ATCTATGCTT CAACAGATAA TTCCACCTGG GTATGCTTCG
    CAGCCAGTTT CACAACAGGT GTGGCCATCT TCAACTCCAG TCTTTTCAGA ATCTCTCTTA
    AAGCATCAGG ATGTTTACCA AAGTGCGGTG GCGGCACCAC AGACTCAATA CAAAGAACCA
    GAGTCAGAGC AGAGTTTGCA GTCTGCATGC CAAGCAGGGG CACCTGAACA GCATGTGTTT
    CAGAAACAAG ATTCTCTTCA GAGTAATGCT CATGGTGACT GTGCCAAAGA CAGCCCAGGT
    GCACACGACA ACACAGCTGG AAATGGCAAG CAAGATAAAA TGAAACAACG GCGAGCTTCC
    TGCCCCCGGC CTGAAAAAAT AGCCAGATTT ATCCTTACTG TAATGCAGGT ATCAACCTCT
    GGAGATAATA TGGTTGAGTG CCAGCTGGAA ACTCATAACA ACAAGATGGT GACTTTTAAA
    TTTGATGCAG ATGGTGATGC TCCGGAAGAT ATTGCCCATT ATATGGTGGA AGATGACTTT
    GTGCTGGAAG TTGAGAAAGA GAAGTTTGTC GAGGAGCTTA GGAATATAGT TACACAGGCC
    CAGGAGATAC TGCGCAGCAT ACCCGCTGAG GAGAGAACTG AATTGATACA GTCAGAGTCT
    AACAGTCAGG CTGGATCCTC AGATCATGTA CAGATGGGAG TCCCAACATT CCAGCAAACG
    GGGGGTGAAA CGGTTCCACA GTCCTCCCCA GTTGGGCGCT GGAGGTTTTT CATCAATCAG
    ACCATAAGGA ACCGCGAATC TCAGACCTCC TCTGTTTCAC AGTCTGCTGT GATCAGGGTT
    CCTCAATTAG CCACATATAA TGAACAAGCA AGTCTCCCTA AGGATTCTTC AGCAGACAGC
    TGTTCTCTTA AATCTGAGCA TACAGTGCCG ACTGTTCTTC TTCAGCCAAC AGATGGTCAT
    GCTTTCCCTA CTGAGACTGA ACCTTTGACT CTCCCTACTT ATGTGACAGT TGGAAGTGAA
    ATGTTATCAT CTCCATTGGA CTGTAATGCC TCAACTTTAG GTCAGATTCA GCTAGAACCT
    AGAAACTGTG GTAGTAATAT ACTTGTGCTA GCAGAGAGCC AGCAGCCAGA AATACCTTCA
    TCTTTTGATC AGGACGTCCA AGGAGCAAAT GGCCTGGTTA ACACAACTGA GACCAGTACT
    GGACACTTAC CGGCACAAAG CACTACAGGC TTGTTACTTG GCTTAACTAT GTCAGATGCT
    CTTCATCCAG TTGATACAAC TGTGTTGCCC CAGACTCCCT TGTCAGAAGT TTCCTTAGCG
    CACTCTGCAT CAGTGCAAGA GTCTGACACT GAAGGACCCC CTAAAATAGA CTATGTGGAT
    AATCACATCA AAACGTTGGA TGAGAAATTA AGAACTTTGT TATATCAGGA CAGCTCTGCC
    AGCTCTTATG CTGACAGCCA AAAGGAAACA CAGAGTATAG AATCTCCCTT ATCTTCATCT
    GCTGAGGATA CTTTGTCGTG TCCTGTCCAT GAAGCCCTCG ATGTGAATAC TGCCGGTGTG
    CAGGCCACTC CAGAGGAGGC AGACGCTAAT GACCCTCCAG CGCAAAGAGA TCCTGGAGAA
    GTCATCACAG TACCTGGTAC ACTAACACCA TCAGAGAGTT CAGAAGATGC CTGGGTTCCT
    GGAAGTTCCT CCCACGCTTG TTCCAAGCGC TCTATGGGTA CTGGAGCCAC GCATCTGCAA
    TCAGGAGGTG GATATTTTGG CCTAAGCTTT ACTTGTCCTA GTCTCAAAAA TCCCATTAGC
    AAGAAACCGT GGGCTCGCAA ATTAAAAAGC TGGGCATGCA GATTGCGGCA TTCCAACAGC
    TTATTCAAGA GGTCAAGAGT CCGGCAAGTT GAAGAGGAGG GAGTGAGACT GGACATATCT
    GATGCCACAC AAAGAGTTGT TGAAGCATTT GCTGAATGTG CCTTGTCAGA AGGGTCGCCA
    TCAAAAAACG GCACCTTCAA GCGTGGCCGT TTCCAGGTTG TTTCCGTTCC ACAGCAAGAG
    CAGCCCACTG CAAATGAATC AGCTGGTGCT CCAGATACCA AATACCCAGG TAACTCTCTT
    TTACAAGAAA CTGGATCCGC AAATACAAAA GCAGAAGACA CTCCGCAGTC TGCCAGCACA
    GCATGTGAGA CTGATGCCTC CTCCCTCACA CCTGATAGAG AACTGGATGA AACCTCAGCC
    AATGGCAGCA GTGTTCAGTC AAGTTCAGCC TTGTGGATGA AAGATATCAA GAAGCCGAGC
    AGCTGTAATA AACAGCTAAG CAGTGATTCT GAACAATCTA CTACTCCGGA CAGGCCCCTG
    GAATTTAAAG GGAGAGAAAA AGATGCGGGG CAGCAACCCC AGGGAGAGAA AATGTATAAC
    CAGAAACAGA ATTCCTTCTT TCACTCTCCA TCTTCCCCCA TGAGCAGTGA TGACGAGTCC
    GAGTTAGAAG ATGAAGATTT GAAAGTGGAA CTTCAGAAGC TCCGAGAAAA GCACATTCAA
    GAGGTGGTGA CCCTTCAAAC CCAGCAAAAC CACGAGCTCC AGGAGCTGTA TGTACGCTTG
    AAGTCTTTAA GGGAAAGCAA AGCACAGTCT TCAGATACCT CTTCCCAGCC ATTATCCCCC
    CGTCGCCCAA GGTCTCTAAA GAGTAAACTG CGCAGCAGGC CCCAGTCCCT CACTCACATG
    GACAATGGGA TCGGGCTTTC AGACCACCAG TGCAGCGAGA GCAATTCCGA TGCTTGCCAG
    CAGTCCTTGT CTGAGAAGAA GAGCATGTTC ACGGATGACT TTCATAAGCT GGTGGATGAC
    TGGGCAAAGG AAAATGCTGG CAACCAAGTT TTGAAACCCA GTCTGAATCA GATCAAGCAG
    AATCAAAGTC GCCTAGAGTC TGATAACTGG GGTCGGACCT ATGAGAGTAT GACAGCCACA
    TCAGGCTATC CACCTGCATG GATCCCTCAG ATTCATGGCT CTTTGCCAGC TGCTGTCTCT
    CAGTCTCTCG TGCCCACCAA CTTTACAGCT GGAGCGATGC CAGCATACCA AGTGCCACCA
    GCCTGCCAAT TCACTTCAGT GAGCAGTAGT GGTTATCCTG TACCATGGTC TGCGCAAGCA
    CCAGTAGTCC CTCCCCAGCA TCTCACAGCA TACCAGCCTG GCGTCGGAGT TCAAACTTTT
    GCAACCTCAA CAGCTCAGAA GGCAACTACA ATCCCAACAT CTCCAAAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.