SACM1L cDNA ORF clone, Xenopus tropicalis(Tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	SACM1L
Entrez Gene ID	100497354
Full Name	SAC1 like phosphatidylinositide phosphatase
Synonyms	sac1
General protein information	Preferred Names SAC1 like phosphatidylinositide phosphatase Names phosphatidylinositide phosphatase SAC1 SAC1 (suppressor of actin mutations 1, homolog)-like SAC1 suppressor of actin mutations 1 like SAC1 suppressor of actin mutations 1-like
Gene Type	protein-coding
Organism	Xenopus tropicalis(Tropical clawed frog)
Summary	This gene encodes an integral membrane protein, which is localized to the endoplasmic reticulum, and functions as a phosphoinositide phosphatase that hydrolyzes phosphatidylinositol 3-phosphate, phosphatidylinositol 4-phosphate, and phosphatidylinositol 3,5-bisphosphate. Deletion of this gene in mouse results in preimplantation lethality. Other studies suggest that this gene is also involved in the organization of golgi membranes and mitotic spindles. Two isoforms are predicted to be produced from the same mRNA by the use of alternative in-frame translation termination codons via a stop codon readthrough mechanism. [provided by RefSeq, Dec 2017].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001357586.1	NP_001344515.1	phosphatidylinositide phosphatase SAC1 isoform 1
NM_001357587.1	NP_001344516.1	phosphatidylinositide phosphatase SAC1 isoform 1x
XM_018095013.1	XP_017950502.1	phosphatidylinositide phosphatase SAC1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Evidence of efficient stop codon readthrough in four mammalian genes.
Loughran G, Chou MY, Ivanov IP, Jungreis I, Kellis M, Kiran AM, Baranov PV, Atkins JF
Nucleic acids research42(14)8928-38(2014 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SACM1L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SACM1L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa45165	NM_001357586.1 Latest version!	Xenopus tropicalis SAC1 like phosphatidylinositide phosphatase (sacm1l), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OXa47207	NM_001357587.1 Latest version!	Xenopus tropicalis SAC1 like phosphatidylinositide phosphatase (sacm1l), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OXa45165	XM_018095013.1 Latest version!	Xenopus tropicalis SAC1 (suppressor of actin mutations 1, homolog)-like (sacm1l), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SACM1L ( NM_001357586.1 ) cDNA ORF clone, Xenopus tropicalis(Tropical clawed frog) -> NP_001344515.1 Xenopus tropicalis SAC1 like phosphatidylinositide phosphatase (sacm1l), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OXa45165
Clone ID Related Accession (Same CDS sequence)	NM_001357586.1 , XM_018095013.1
Accession Version	NM_001357586.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-18
Organism	Xenopus tropicalis(tropical clawed frog)
Product	phosphatidylinositide phosphatase SAC1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL964894.2, CX898144.1, BX740915.1, CX500573.2, EL677126.1, BX741505.1, BX741506.1, AAMC03024096.1 and CX386955.1. On Oct 31, 2017 this sequence version replaced XM_018095013.1. Transcript Variant: This variant (1) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon, while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UGA termination codon. This RefSeq represents the shorter isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. COMPLETENESS: complete on the 3' end.

ATGGCACTTG CCTACGAAAA TCTGAAATTA CATATAACTC CTGAAAAGTT TTATGTTGAA 
GCATGTGATC CTGGAGTAGA TGATGTCCTT ACTATAGACA GAGTGTCAAC AGAGGTCACA 
CTTACAGGTA AAAAGGACAT ACCCCCATCA GCAATAACAA GAGCTATATA TGGAATTTTA 
GGCACAATTC GACTAGTTGC AGGAATGTAT TTGATTGTGA TAACCCGAAA GAAGAAAGTC 
GGTGATCTTC TTAATCATTC TATCTGGAAA GCAACAGACT TTGACATAAT TTCTTATAAA 
AAGACAATGT TACATTTAAC TGATACTCAG TTGCAGGATA ACAAGGCCTT CCTTGCGATG 
ATCAGTCATG TTCTGAGTGT AGATGGATTT TACTTTTCTG TATCTTATGA CCTTACCCAC 
ACGTTACAAC GGTTATCTAA TACAAGTCCT GAATTCCAAG AAATGAGCCT GCTTGAAAGG 
GCTGATCAGA GATTTGTTTG GAATGGTCAT CTTTTAAGGG AATTTTCAGC TCAACCTGAG 
ATCCAAAAAT TTGCGATTCC AGTGGTTCAT GGATTTATTG CTATACATTC TTGTTCTATA 
AATGGCAAAT ACTTTGACTG GATTCTCATC TCTAGGAGGA GCTGTTTTCG AGCAGGTGTA 
CGGTATTATG TAAGAGGTAT TGATTCAGAG GGGCATGCTG CAAACTTTGT TGAAACAGAG 
CAGATTGTTC ATTACAATGG AAACAAAGCT TCATTTGTTC AGACACGTGG ATCTATTCCA 
TTTTACTGGT CACAGAGACC AAATCTCAAA TATAAACCAA AGCCTCAGAT CAGCAAAGCA 
GTGAACCATA TGGATGGATT CCAACGGCAC TTTGACTCAC AGATCATAAG TTATGGGAAG 
CAAGTTGTTC TAAACTTGGT TAACCAAAAG GGTTCAGAAA AGCCATTAGA GCAAGCTTTT 
TCACAGATGG TTTCTGGCCT TGGAAATGGA ATGCTTAGAT ATATTGCATT TGACTTTCAT 
AAAGAATGCA GTAGAATGCG CTGGGATCGC TTACAGATTT TGGTTGATCA AGTTTCTGAG 
ACTCAAGATG ATTTTGGCTA TTTTCTGGTA GACTCTGATG GCAAAGTGGT TAGCCAACAG 
GAAGGGATTT TCCGCAGTAA CTGTATGGAC TGTCTGGATC GCACAAATGT AGTGCAGAGT 
CTTTTGGCCC GACGTTCCCT CCAGTTCCAG CTTCAGAGGC TTGGTGTGCT TCATGTTGGA 
CAAAGGATTG AAGAACAAAT TCAGTTTGAA AAGATCTATA AAAATGCATG GGCTGACAAT 
GCAAATGCAT GTGCCAAACA GTATGCTGGA ACTGGTGCTT TAAAGACAGA CTTCACAAGG 
ACTGGAAAAC GAACTCAGTG GGGACTTTTA ATGGATGGTT GGAACTCATT AATGCGATAT 
TATAAAAATA ATTTTTCTGA TGGCTTCCGA CAAGACTCAA TTGACTTGTT TCTTGGCAAT 
TACTCTGTTG AAGAAGTCTA TTCTTCAAGT CCACTGCATA TACAGACAGA CTGGAAATTT 
CTTGCATTAC CTATTATCAT GGTGGTTGCC TTTTCCATGT GCATTATCTG CTTATTAATG 
GCTGGTGACA CATGGACAGA GACTTTGGCT TATGTTCTAT TCTGGGGCAC TGCAAGTATT 
GGAACAGGCA CTATCATTCT GTACAATGGC AAAGATTTCG TTGATGCCCC AAAGCTGGTA 
CAGAAGGAAA AGATGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001344515.1
CDS	147..1907
Translation	MALAYENLKLHITPEKFYVEACDPGVDDVLTIDRVSTEVTLTGKKDIPPSAITRAIYGILGTIRLVAGMYLIVITRKKKVGDLLNHSIWKATDFDIISYKKTMLHLTDTQLQDNKAFLAMISHVLSVDGFYFSVSYDLTHTLQRLSNTSPEFQEMSLLERADQRFVWNGHLLREFSAQPEIQKFAIPVVHGFIAIHSCSINGKYFDWILISRRSCFRAGVRYYVRGIDSEGHAANFVETEQIVHYNGNKASFVQTRGSIPFYWSQRPNLKYKPKPQISKAVNHMDGFQRHFDSQIISYGKQVVLNLVNQKGSEKPLEQAFSQMVSGLGNGMLRYIAFDFHKECSRMRWDRLQILVDQVSETQDDFGYFLVDSDGKVVSQQEGIFRSNCMDCLDRTNVVQSLLARRSLQFQLQRLGVLHVGQRIEEQIQFEKIYKNAWADNANACAKQYAGTGALKTDFTRTGKRTQWGLLMDGWNSLMRYYKNNFSDGFRQDSIDLFLGNYSVEEVYSSSPLHIQTDWKFLALPIIMVVAFSMCIICLLMAGDTWTETLAYVLFWGTASIGTGTIILYNGKDFVDAPKLVQKEKMD

Target ORF information:

RefSeq Version	NM_001357586.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis SAC1 like phosphatidylinositide phosphatase (sacm1l), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001357586.1

ORF Insert Sequence:

ATGGCACTTG CCTACGAAAA TCTGAAATTA CATATAACTC CTGAAAAGTT TTATGTTGAA 
GCATGTGATC CTGGAGTAGA TGATGTCCTT ACTATAGACA GAGTGTCAAC AGAGGTCACA 
CTTACAGGTA AAAAGGACAT ACCCCCATCA GCAATAACAA GAGCTATATA TGGAATTTTA 
GGCACAATTC GACTAGTTGC AGGAATGTAT TTGATTGTGA TAACCCGAAA GAAGAAAGTC 
GGTGATCTTC TTAATCATTC TATCTGGAAA GCAACAGACT TTGACATAAT TTCTTATAAA 
AAGACAATGT TACATTTAAC TGATACTCAG TTGCAGGATA ACAAGGCCTT CCTTGCGATG 
ATCAGTCATG TTCTGAGTGT AGATGGATTT TACTTTTCTG TATCTTATGA CCTTACCCAC 
ACGTTACAAC GGTTATCTAA TACAAGTCCT GAATTCCAAG AAATGAGCCT GCTTGAAAGG 
GCTGATCAGA GATTTGTTTG GAATGGTCAT CTTTTAAGGG AATTTTCAGC TCAACCTGAG 
ATCCAAAAAT TTGCGATTCC AGTGGTTCAT GGATTTATTG CTATACATTC TTGTTCTATA 
AATGGCAAAT ACTTTGACTG GATTCTCATC TCTAGGAGGA GCTGTTTTCG AGCAGGTGTA 
CGGTATTATG TAAGAGGTAT TGATTCAGAG GGGCATGCTG CAAACTTTGT TGAAACAGAG 
CAGATTGTTC ATTACAATGG AAACAAAGCT TCATTTGTTC AGACACGTGG ATCTATTCCA 
TTTTACTGGT CACAGAGACC AAATCTCAAA TATAAACCAA AGCCTCAGAT CAGCAAAGCA 
GTGAACCATA TGGATGGATT CCAACGGCAC TTTGACTCAC AGATCATAAG TTATGGGAAG 
CAAGTTGTTC TAAACTTGGT TAACCAAAAG GGTTCAGAAA AGCCATTAGA GCAAGCTTTT 
TCACAGATGG TTTCTGGCCT TGGAAATGGA ATGCTTAGAT ATATTGCATT TGACTTTCAT 
AAAGAATGCA GTAGAATGCG CTGGGATCGC TTACAGATTT TGGTTGATCA AGTTTCTGAG 
ACTCAAGATG ATTTTGGCTA TTTTCTGGTA GACTCTGATG GCAAAGTGGT TAGCCAACAG 
GAAGGGATTT TCCGCAGTAA CTGTATGGAC TGTCTGGATC GCACAAATGT AGTGCAGAGT 
CTTTTGGCCC GACGTTCCCT CCAGTTCCAG CTTCAGAGGC TTGGTGTGCT TCATGTTGGA 
CAAAGGATTG AAGAACAAAT TCAGTTTGAA AAGATCTATA AAAATGCATG GGCTGACAAT 
GCAAATGCAT GTGCCAAACA GTATGCTGGA ACTGGTGCTT TAAAGACAGA CTTCACAAGG 
ACTGGAAAAC GAACTCAGTG GGGACTTTTA ATGGATGGTT GGAACTCATT AATGCGATAT 
TATAAAAATA ATTTTTCTGA TGGCTTCCGA CAAGACTCAA TTGACTTGTT TCTTGGCAAT 
TACTCTGTTG AAGAAGTCTA TTCTTCAAGT CCACTGCATA TACAGACAGA CTGGAAATTT 
CTTGCATTAC CTATTATCAT GGTGGTTGCC TTTTCCATGT GCATTATCTG CTTATTAATG 
GCTGGTGACA CATGGACAGA GACTTTGGCT TATGTTCTAT TCTGGGGCAC TGCAAGTATT 
GGAACAGGCA CTATCATTCT GTACAATGGC AAAGATTTCG TTGATGCCCC AAAGCTGGTA 
CAGAAGGAAA AGATGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SACM1L ( NM_001357587.1 ) cDNA ORF clone, Xenopus tropicalis(Tropical clawed frog) -> NP_001344516.1 Xenopus tropicalis SAC1 like phosphatidylinositide phosphatase (sacm1l), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OXa47207
Clone ID Related Accession (Same CDS sequence)	NM_001357587.1
Accession Version	NM_001357587.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-18
Organism	Xenopus tropicalis(tropical clawed frog)
Product	phosphatidylinositide phosphatase SAC1 isoform 1x
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL964894.2, CX898144.1, BX740915.1, CX500573.2, EL677126.1, BX741505.1, BX741506.1, AAMC03024096.1 and CX386955.1. Transcript Variant: This variant (1) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon, while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UGA termination codon. This RefSeq represents the longer isoform (1x). As the UGA stop codon has been reported to specify several alternative amino acids (tryptophan, cysteine, arginine and serine), its location in the longer isoform is denoted by an 'X'. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##RefSeq-Attributes-START## stop codon readthrough :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCACTTG CCTACGAAAA TCTGAAATTA CATATAACTC CTGAAAAGTT TTATGTTGAA 
GCATGTGATC CTGGAGTAGA TGATGTCCTT ACTATAGACA GAGTGTCAAC AGAGGTCACA 
CTTACAGGTA AAAAGGACAT ACCCCCATCA GCAATAACAA GAGCTATATA TGGAATTTTA 
GGCACAATTC GACTAGTTGC AGGAATGTAT TTGATTGTGA TAACCCGAAA GAAGAAAGTC 
GGTGATCTTC TTAATCATTC TATCTGGAAA GCAACAGACT TTGACATAAT TTCTTATAAA 
AAGACAATGT TACATTTAAC TGATACTCAG TTGCAGGATA ACAAGGCCTT CCTTGCGATG 
ATCAGTCATG TTCTGAGTGT AGATGGATTT TACTTTTCTG TATCTTATGA CCTTACCCAC 
ACGTTACAAC GGTTATCTAA TACAAGTCCT GAATTCCAAG AAATGAGCCT GCTTGAAAGG 
GCTGATCAGA GATTTGTTTG GAATGGTCAT CTTTTAAGGG AATTTTCAGC TCAACCTGAG 
ATCCAAAAAT TTGCGATTCC AGTGGTTCAT GGATTTATTG CTATACATTC TTGTTCTATA 
AATGGCAAAT ACTTTGACTG GATTCTCATC TCTAGGAGGA GCTGTTTTCG AGCAGGTGTA 
CGGTATTATG TAAGAGGTAT TGATTCAGAG GGGCATGCTG CAAACTTTGT TGAAACAGAG 
CAGATTGTTC ATTACAATGG AAACAAAGCT TCATTTGTTC AGACACGTGG ATCTATTCCA 
TTTTACTGGT CACAGAGACC AAATCTCAAA TATAAACCAA AGCCTCAGAT CAGCAAAGCA 
GTGAACCATA TGGATGGATT CCAACGGCAC TTTGACTCAC AGATCATAAG TTATGGGAAG 
CAAGTTGTTC TAAACTTGGT TAACCAAAAG GGTTCAGAAA AGCCATTAGA GCAAGCTTTT 
TCACAGATGG TTTCTGGCCT TGGAAATGGA ATGCTTAGAT ATATTGCATT TGACTTTCAT 
AAAGAATGCA GTAGAATGCG CTGGGATCGC TTACAGATTT TGGTTGATCA AGTTTCTGAG 
ACTCAAGATG ATTTTGGCTA TTTTCTGGTA GACTCTGATG GCAAAGTGGT TAGCCAACAG 
GAAGGGATTT TCCGCAGTAA CTGTATGGAC TGTCTGGATC GCACAAATGT AGTGCAGAGT 
CTTTTGGCCC GACGTTCCCT CCAGTTCCAG CTTCAGAGGC TTGGTGTGCT TCATGTTGGA 
CAAAGGATTG AAGAACAAAT TCAGTTTGAA AAGATCTATA AAAATGCATG GGCTGACAAT 
GCAAATGCAT GTGCCAAACA GTATGCTGGA ACTGGTGCTT TAAAGACAGA CTTCACAAGG 
ACTGGAAAAC GAACTCAGTG GGGACTTTTA ATGGATGGTT GGAACTCATT AATGCGATAT 
TATAAAAATA ATTTTTCTGA TGGCTTCCGA CAAGACTCAA TTGACTTGTT TCTTGGCAAT 
TACTCTGTTG AAGAAGTCTA TTCTTCAAGT CCACTGCATA TACAGACAGA CTGGAAATTT 
CTTGCATTAC CTATTATCAT GGTGGTTGCC TTTTCCATGT GCATTATCTG CTTATTAATG 
GCTGGTGACA CATGGACAGA GACTTTGGCT TATGTTCTAT TCTGGGGCAC TGCAAGTATT 
GGAACAGGCA CTATCATTCT GTACAATGGC AAAGATTTCG TTGATGCCCC AAAGCTGGTA 
CAGAAGGAAA AGATGGACTG AATTTGTGTG TGTGGAAAAC AGCTTAGCAG TGACGGTTCA 
TTAAACCCAG TATGGAGTCT TTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001344516.1
CDS	147..1973
Translation	MALAYENLKLHITPEKFYVEACDPGVDDVLTIDRVSTEVTLTGKKDIPPSAITRAIYGILGTIRLVAGMYLIVITRKKKVGDLLNHSIWKATDFDIISYKKTMLHLTDTQLQDNKAFLAMISHVLSVDGFYFSVSYDLTHTLQRLSNTSPEFQEMSLLERADQRFVWNGHLLREFSAQPEIQKFAIPVVHGFIAIHSCSINGKYFDWILISRRSCFRAGVRYYVRGIDSEGHAANFVETEQIVHYNGNKASFVQTRGSIPFYWSQRPNLKYKPKPQISKAVNHMDGFQRHFDSQIISYGKQVVLNLVNQKGSEKPLEQAFSQMVSGLGNGMLRYIAFDFHKECSRMRWDRLQILVDQVSETQDDFGYFLVDSDGKVVSQQEGIFRSNCMDCLDRTNVVQSLLARRSLQFQLQRLGVLHVGQRIEEQIQFEKIYKNAWADNANACAKQYAGTGALKTDFTRTGKRTQWGLLMDGWNSLMRYYKNNFSDGFRQDSIDLFLGNYSVEEVYSSSPLHIQTDWKFLALPIIMVVAFSMCIICLLMAGDTWTETLAYVLFWGTASIGTGTIILYNGKDFVDAPKLVQKEKMD*ICVCGKQLSSDGSLNPVWSLY

Target ORF information:

RefSeq Version	NM_001357587.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis SAC1 like phosphatidylinositide phosphatase (sacm1l), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001357587.1

ORF Insert Sequence:

ATGGCACTTG CCTACGAAAA TCTGAAATTA CATATAACTC CTGAAAAGTT TTATGTTGAA 
GCATGTGATC CTGGAGTAGA TGATGTCCTT ACTATAGACA GAGTGTCAAC AGAGGTCACA 
CTTACAGGTA AAAAGGACAT ACCCCCATCA GCAATAACAA GAGCTATATA TGGAATTTTA 
GGCACAATTC GACTAGTTGC AGGAATGTAT TTGATTGTGA TAACCCGAAA GAAGAAAGTC 
GGTGATCTTC TTAATCATTC TATCTGGAAA GCAACAGACT TTGACATAAT TTCTTATAAA 
AAGACAATGT TACATTTAAC TGATACTCAG TTGCAGGATA ACAAGGCCTT CCTTGCGATG 
ATCAGTCATG TTCTGAGTGT AGATGGATTT TACTTTTCTG TATCTTATGA CCTTACCCAC 
ACGTTACAAC GGTTATCTAA TACAAGTCCT GAATTCCAAG AAATGAGCCT GCTTGAAAGG 
GCTGATCAGA GATTTGTTTG GAATGGTCAT CTTTTAAGGG AATTTTCAGC TCAACCTGAG 
ATCCAAAAAT TTGCGATTCC AGTGGTTCAT GGATTTATTG CTATACATTC TTGTTCTATA 
AATGGCAAAT ACTTTGACTG GATTCTCATC TCTAGGAGGA GCTGTTTTCG AGCAGGTGTA 
CGGTATTATG TAAGAGGTAT TGATTCAGAG GGGCATGCTG CAAACTTTGT TGAAACAGAG 
CAGATTGTTC ATTACAATGG AAACAAAGCT TCATTTGTTC AGACACGTGG ATCTATTCCA 
TTTTACTGGT CACAGAGACC AAATCTCAAA TATAAACCAA AGCCTCAGAT CAGCAAAGCA 
GTGAACCATA TGGATGGATT CCAACGGCAC TTTGACTCAC AGATCATAAG TTATGGGAAG 
CAAGTTGTTC TAAACTTGGT TAACCAAAAG GGTTCAGAAA AGCCATTAGA GCAAGCTTTT 
TCACAGATGG TTTCTGGCCT TGGAAATGGA ATGCTTAGAT ATATTGCATT TGACTTTCAT 
AAAGAATGCA GTAGAATGCG CTGGGATCGC TTACAGATTT TGGTTGATCA AGTTTCTGAG 
ACTCAAGATG ATTTTGGCTA TTTTCTGGTA GACTCTGATG GCAAAGTGGT TAGCCAACAG 
GAAGGGATTT TCCGCAGTAA CTGTATGGAC TGTCTGGATC GCACAAATGT AGTGCAGAGT 
CTTTTGGCCC GACGTTCCCT CCAGTTCCAG CTTCAGAGGC TTGGTGTGCT TCATGTTGGA 
CAAAGGATTG AAGAACAAAT TCAGTTTGAA AAGATCTATA AAAATGCATG GGCTGACAAT 
GCAAATGCAT GTGCCAAACA GTATGCTGGA ACTGGTGCTT TAAAGACAGA CTTCACAAGG 
ACTGGAAAAC GAACTCAGTG GGGACTTTTA ATGGATGGTT GGAACTCATT AATGCGATAT 
TATAAAAATA ATTTTTCTGA TGGCTTCCGA CAAGACTCAA TTGACTTGTT TCTTGGCAAT 
TACTCTGTTG AAGAAGTCTA TTCTTCAAGT CCACTGCATA TACAGACAGA CTGGAAATTT 
CTTGCATTAC CTATTATCAT GGTGGTTGCC TTTTCCATGT GCATTATCTG CTTATTAATG 
GCTGGTGACA CATGGACAGA GACTTTGGCT TATGTTCTAT TCTGGGGCAC TGCAAGTATT 
GGAACAGGCA CTATCATTCT GTACAATGGC AAAGATTTCG TTGATGCCCC AAAGCTGGTA 
CAGAAGGAAA AGATGGACTG AATTTGTGTG TGTGGAAAAC AGCTTAGCAG TGACGGTTCA 
TTAAACCCAG TATGGAGTCT TTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SACM1L ( XM_018095013.1 ) cDNA ORF clone, Xenopus tropicalis(Tropical clawed frog) -> XP_017950502.1 Xenopus tropicalis SAC1 (suppressor of actin mutations 1, homolog)-like (sacm1l), mRNA.

Price & Availability↑

CloneID	OXa45165
Clone ID Related Accession (Same CDS sequence)	NM_001357586.1 , XM_018095013.1
Accession Version	XM_018095013.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(Tropical clawed frog)
Product	phosphatidylinositide phosphatase SAC1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTTG CCTACGAAAA TCTGAAATTA CATATAACTC CTGAAAAGTT TTATGTTGAA 
GCATGTGATC CTGGAGTAGA TGATGTCCTT ACTATAGACA GAGTGTCAAC AGAGGTCACA 
CTTACAGGTA AAAAGGACAT ACCCCCATCA GCAATAACAA GAGCTATATA TGGAATTTTA 
GGCACAATTC GACTAGTTGC AGGAATGTAT TTGATTGTGA TAACCCGAAA GAAGAAAGTC 
GGTGATCTTC TTAATCATTC TATCTGGAAA GCAACAGACT TTGACATAAT TTCTTATAAA 
AAGACAATGT TACATTTAAC TGATACTCAG TTGCAGGATA ACAAGGCCTT CCTTGCGATG 
ATCAGTCATG TTCTGAGTGT AGATGGATTT TACTTTTCTG TATCTTATGA CCTTACCCAC 
ACGTTACAAC GGTTATCTAA TACAAGTCCT GAATTCCAAG AAATGAGCCT GCTTGAAAGG 
GCTGATCAGA GATTTGTTTG GAATGGTCAT CTTTTAAGGG AATTTTCAGC TCAACCTGAG 
ATCCAAAAAT TTGCGATTCC AGTGGTTCAT GGATTTATTG CTATACATTC TTGTTCTATA 
AATGGCAAAT ACTTTGACTG GATTCTCATC TCTAGGAGGA GCTGTTTTCG AGCAGGTGTA 
CGGTATTATG TAAGAGGTAT TGATTCAGAG GGGCATGCTG CAAACTTTGT TGAAACAGAG 
CAGATTGTTC ATTACAATGG AAACAAAGCT TCATTTGTTC AGACACGTGG ATCTATTCCA 
TTTTACTGGT CACAGAGACC AAATCTCAAA TATAAACCAA AGCCTCAGAT CAGCAAAGCA 
GTGAACCATA TGGATGGATT CCAACGGCAC TTTGACTCAC AGATCATAAG TTATGGGAAG 
CAAGTTGTTC TAAACTTGGT TAACCAAAAG GGTTCAGAAA AGCCATTAGA GCAAGCTTTT 
TCACAGATGG TTTCTGGCCT TGGAAATGGA ATGCTTAGAT ATATTGCATT TGACTTTCAT 
AAAGAATGCA GTAGAATGCG CTGGGATCGC TTACAGATTT TGGTTGATCA AGTTTCTGAG 
ACTCAAGATG ATTTTGGCTA TTTTCTGGTA GACTCTGATG GCAAAGTGGT TAGCCAACAG 
GAAGGGATTT TCCGCAGTAA CTGTATGGAC TGTCTGGATC GCACAAATGT AGTGCAGAGT 
CTTTTGGCCC GACGTTCCCT CCAGTTCCAG CTTCAGAGGC TTGGTGTGCT TCATGTTGGA 
CAAAGGATTG AAGAACAAAT TCAGTTTGAA AAGATCTATA AAAATGCATG GGCTGACAAT 
GCAAATGCAT GTGCCAAACA GTATGCTGGA ACTGGTGCTT TAAAGACAGA CTTCACAAGG 
ACTGGAAAAC GAACTCAGTG GGGACTTTTA ATGGATGGTT GGAACTCATT AATGCGATAT 
TATAAAAATA ATTTTTCTGA TGGCTTCCGA CAAGACTCAA TTGACTTGTT TCTTGGCAAT 
TACTCTGTTG AAGAAGTCTA TTCTTCAAGT CCACTGCATA TACAGACAGA CTGGAAATTT 
CTTGCATTAC CTATTATCAT GGTGGTTGCC TTTTCCATGT GCATTATCTG CTTATTAATG 
GCTGGTGACA CATGGACAGA GACTTTGGCT TATGTTCTAT TCTGGGGCAC TGCAAGTATT 
GGAACAGGCA CTATCATTCT GTACAATGGC AAAGATTTCG TTGATGCCCC AAAGCTGGTA 
CAGAAGGAAA AGATGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017950502.1
CDS	198..1958
Translation	MALAYENLKLHITPEKFYVEACDPGVDDVLTIDRVSTEVTLTGKKDIPPSAITRAIYGILGTIRLVAGMYLIVITRKKKVGDLLNHSIWKATDFDIISYKKTMLHLTDTQLQDNKAFLAMISHVLSVDGFYFSVSYDLTHTLQRLSNTSPEFQEMSLLERADQRFVWNGHLLREFSAQPEIQKFAIPVVHGFIAIHSCSINGKYFDWILISRRSCFRAGVRYYVRGIDSEGHAANFVETEQIVHYNGNKASFVQTRGSIPFYWSQRPNLKYKPKPQISKAVNHMDGFQRHFDSQIISYGKQVVLNLVNQKGSEKPLEQAFSQMVSGLGNGMLRYIAFDFHKECSRMRWDRLQILVDQVSETQDDFGYFLVDSDGKVVSQQEGIFRSNCMDCLDRTNVVQSLLARRSLQFQLQRLGVLHVGQRIEEQIQFEKIYKNAWADNANACAKQYAGTGALKTDFTRTGKRTQWGLLMDGWNSLMRYYKNNFSDGFRQDSIDLFLGNYSVEEVYSSSPLHIQTDWKFLALPIIMVVAFSMCIICLLMAGDTWTETLAYVLFWGTASIGTGTIILYNGKDFVDAPKLVQKEKMD

Target ORF information:

RefSeq Version	XM_018095013.1
Organism	Xenopus tropicalis(Tropical clawed frog)
Definition	Xenopus tropicalis SAC1 (suppressor of actin mutations 1, homolog)-like (sacm1l), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095013.1

ORF Insert Sequence:

ATGGCACTTG CCTACGAAAA TCTGAAATTA CATATAACTC CTGAAAAGTT TTATGTTGAA 
GCATGTGATC CTGGAGTAGA TGATGTCCTT ACTATAGACA GAGTGTCAAC AGAGGTCACA 
CTTACAGGTA AAAAGGACAT ACCCCCATCA GCAATAACAA GAGCTATATA TGGAATTTTA 
GGCACAATTC GACTAGTTGC AGGAATGTAT TTGATTGTGA TAACCCGAAA GAAGAAAGTC 
GGTGATCTTC TTAATCATTC TATCTGGAAA GCAACAGACT TTGACATAAT TTCTTATAAA 
AAGACAATGT TACATTTAAC TGATACTCAG TTGCAGGATA ACAAGGCCTT CCTTGCGATG 
ATCAGTCATG TTCTGAGTGT AGATGGATTT TACTTTTCTG TATCTTATGA CCTTACCCAC 
ACGTTACAAC GGTTATCTAA TACAAGTCCT GAATTCCAAG AAATGAGCCT GCTTGAAAGG 
GCTGATCAGA GATTTGTTTG GAATGGTCAT CTTTTAAGGG AATTTTCAGC TCAACCTGAG 
ATCCAAAAAT TTGCGATTCC AGTGGTTCAT GGATTTATTG CTATACATTC TTGTTCTATA 
AATGGCAAAT ACTTTGACTG GATTCTCATC TCTAGGAGGA GCTGTTTTCG AGCAGGTGTA 
CGGTATTATG TAAGAGGTAT TGATTCAGAG GGGCATGCTG CAAACTTTGT TGAAACAGAG 
CAGATTGTTC ATTACAATGG AAACAAAGCT TCATTTGTTC AGACACGTGG ATCTATTCCA 
TTTTACTGGT CACAGAGACC AAATCTCAAA TATAAACCAA AGCCTCAGAT CAGCAAAGCA 
GTGAACCATA TGGATGGATT CCAACGGCAC TTTGACTCAC AGATCATAAG TTATGGGAAG 
CAAGTTGTTC TAAACTTGGT TAACCAAAAG GGTTCAGAAA AGCCATTAGA GCAAGCTTTT 
TCACAGATGG TTTCTGGCCT TGGAAATGGA ATGCTTAGAT ATATTGCATT TGACTTTCAT 
AAAGAATGCA GTAGAATGCG CTGGGATCGC TTACAGATTT TGGTTGATCA AGTTTCTGAG 
ACTCAAGATG ATTTTGGCTA TTTTCTGGTA GACTCTGATG GCAAAGTGGT TAGCCAACAG 
GAAGGGATTT TCCGCAGTAA CTGTATGGAC TGTCTGGATC GCACAAATGT AGTGCAGAGT 
CTTTTGGCCC GACGTTCCCT CCAGTTCCAG CTTCAGAGGC TTGGTGTGCT TCATGTTGGA 
CAAAGGATTG AAGAACAAAT TCAGTTTGAA AAGATCTATA AAAATGCATG GGCTGACAAT 
GCAAATGCAT GTGCCAAACA GTATGCTGGA ACTGGTGCTT TAAAGACAGA CTTCACAAGG 
ACTGGAAAAC GAACTCAGTG GGGACTTTTA ATGGATGGTT GGAACTCATT AATGCGATAT 
TATAAAAATA ATTTTTCTGA TGGCTTCCGA CAAGACTCAA TTGACTTGTT TCTTGGCAAT 
TACTCTGTTG AAGAAGTCTA TTCTTCAAGT CCACTGCATA TACAGACAGA CTGGAAATTT 
CTTGCATTAC CTATTATCAT GGTGGTTGCC TTTTCCATGT GCATTATCTG CTTATTAATG 
GCTGGTGACA CATGGACAGA GACTTTGGCT TATGTTCTAT TCTGGGGCAC TGCAAGTATT 
GGAACAGGCA CTATCATTCT GTACAATGGC AAAGATTTCG TTGATGCCCC AAAGCTGGTA 
CAGAAGGAAA AGATGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evidence of efficient stop codon readthrough in four mammalian genes.
Nucleic acids research42(14)8928-38(2014 Aug)
Loughran G,Chou MY,Ivanov IP,Jungreis I,Kellis M,Kiran AM,Baranov PV,Atkins JF

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SACM1L cDNA ORF clone, Xenopus tropicalis(Tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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